LOCUS       DASUGB010000001         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1528179, whole genome shotgun sequence.
ACCESSION   DASUGB010000001 DASUGB010000000
VERSION     DASUGB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528179"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..>3214)
                     /locus_tag="VFV87_00005"
     CDS             complement(148..>3214)
                     /locus_tag="VFV87_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102160.1"
                     /translation="YPTAAALADDLAHYLRGEPILARPVGRVERAVRWCNRNRMVAGL
                     MGLAVLLLVGGMAVSTTLAIYANYQKADAVQQKGIADANNRKAQENARKWREQERLAT
                     KNAVLATTAAEQAKKSAIAATESAAFARKNQYFADVNLAQQVLAAGDVAQAIDLLDNH
                     IPAEGHEDLRGFEWYYLWSLAHSSAAGILWDQEKAGAVREVRSAKVSPDGTMLAVAGG
                     RSNQGEVILYDARTREPLRTLPLQKACVTAVAWSSDSARLAICSGQLNGTSEVKLFSA
                     ATGEVLWEVASPDPPLALALSADGKWLAVGTAKLIQGHGSPPDRFFSTAVGAREGGAR
                     ILSASDGSVVGELPGDVGNVFSLAFSPDGETLAVGNERGEVLLYNAPAREKTGEFHSG
                     LRYVWSLTFSSKGEWLAAAAGFWNEPGEVSLWNTKSRQVERRVLGHRGGTTTVTFGPG
                     DQILATGSFDRTVRLWSVRDGRELTVFYGHRGYVWDVAFSDGGKTLLAGAWRQNGPGE
                     VLAWQVAEGLRFTPLPAGIFSGGGYYWSVAAGGTVIFPTKEGFDIWDANSATRRASVT
                     KKGLRCIDVSPNGRLVAGGGFAEKTGLWNADNGQLIAELPTLTIPYLSFSPDGALLAT
                     CGDDGVLRLWKAATGKPLQALTGHTDVVWSVAFTPDSQRLATASWDGTVRLWEPMTGR
                     ALGAFPGHTEALWQVAFLENGRTLVSRSGSQEQNIGVWDVSRVLPQAESEVLPPPTSR
                     EVMEVYSYALLAAARSQAGEAERAQQALDHMVERAARHLSQLPLIAVYAEGELENLQE
                     IINALLRLQCWREVDKLCRLSEAACARQLAVAPDSAGLQAIQALVQIKGSQAAVGFGQ
                     PLEALPRLRAARQTLLKLIADNPKATSLQGLLAQAENGFAWYVAMQPEVPRDLIDEAV
                     LFARACTARRPAEGIYMNTLGVILLRAELWDEAVAVLQKSCELRSGGDANDWYPLAVA
                     FHRQGKAEEAKMWFDRAEAWLKVNPSNDADLPQLRKEAAALILPPRIQE"
BASE COUNT          579 a         1109 c          956 g          570 t
ORIGIN      
        1 gcagcgccct tcaccacctc ccaagctgac aaccgctctg ggggtaaggg ggccctccgc
       61 ctccttcacc gcctcctgcc ccatccgccc aaattccaac taccgcacca tacgagaact
      121 caacaacatg aataatccgg gttaggtcta ttcctggatc cgcggcggga ggatcagcgc
      181 ggctgcctcc ttgcgcagtt gcgggaggtc ggcatcgttg ctcggattta cctttagcca
      241 ggcttccgcg cgatcgaacc acatcttcgc ttcctccgcc ttgccctgac ggtggaaggc
      301 gaccgccagc ggataccagt cattggcgtc gccgcccgat cgcagctcgc aagacttctg
      361 caaaaccgct acggcttcat cccacagctc ggcccgcagc aagatgacgc cgagcgtatt
      421 catgtagatc ccttccgcgg gccggcgcgc cgtgcaagcc cgggcaaaga gcacggcttc
      481 gtcgatcaag tcgcgaggca cttcgggctg catcgccacg taccaggcaa agccattctc
      541 ggcctgcgcc agcaaaccct gcagcgaggt cgccttgggg ttatccgcaa tcagcttgag
      601 cagcgtctgg cgggccgctc gcagacgcgg gagcgcctca agcggctgtc caaatccgac
      661 cgccgcctgc gagcccttga tctgcacgag cgcctgaatc gcttgcaatc ccgcggaatc
      721 gggggcgacg gcaagctgcc gcgcgcaggc ggcttcggaa aggcggcaca gcttatcgac
      781 ctcgcgccag cattgcaggc gaaggagcgc gttaatgatc tcttgcaggt tctccagctc
      841 cccttcggcg taaaccgcga tgagcggcag ttggctcaaa tgccgcgcgg cacgctccac
      901 catgtggtcc agcgcttgct gggcccgctc agcctccccg gcctgggaac gggcggcagc
      961 caggagcgca taggagtaca cctccatcac ctcgcgactc gtcggcggcg gaagcacttc
     1021 cgactcggcc tgcggcagga ctcggctcac gtcccagacg ccgatattct gttcctgact
     1081 gccgctgcgg ctcaccaagg tgcgcccatt ttctaagaac gccacctgcc agagggcttc
     1141 ggtatggccg ggaaaggcgc ccagcgcgcg gcccgtcatg ggctcccaca ggcgcaccgt
     1201 gccgtcccag ctcgcggtgg ccagacgctg gctatcggga gtgaacgcaa ccgaccagac
     1261 tacgtccgta tggccagtca gcgcctgcag tggcttgccc gttgcggcct tccagagccg
     1321 gaggacgcca tcgtcgccgc aagtggccag gagggcacca tcgggcgaaa acgagagata
     1381 gggaatcgtc agcgtaggca gttccgcgat caactgcccg ttgtcggcgt tccagaggcc
     1441 ggtcttttcc gcaaagcccc cgcccgcgac gaggcgaccg ttcggtgaaa cgtcgatgca
     1501 ccgcaaaccc ttttttgtga cggaggcccg ccgcgtggcc gaattcgcgt cccagatatc
     1561 aaagccttcc ttcgtcggaa agatcacggt gccaccggct gcgacgctcc agtaatagcc
     1621 tcccccgcta aagatgcccg cgggcagggg cgtgaaacga agtccctcgg caacttgcca
     1681 ggccagaacc tcgcccggtc cattctgacg ccaagcgcct gccagcaggg tttttccgcc
     1741 gtcgctgaac gccacatccc aaacatatcc gcgatgccca tagaacaccg tcagttcgcg
     1801 cccgtcgcgc acgctccaca gccgcaccgt ccggtcgaag cttcccgtgg cgaggatctg
     1861 atcgcccgga ccaaaagtca cagtggtcgt cccaccgcgg tggccaagga cgcggcgctc
     1921 cacttggcgc gatttcgtat tccacagcga cacctcgccc ggctcgttcc agaagcccgc
     1981 tgctgctgcg agccactcgc ctttcgacga gaacgtcagc gaccagacat agcggagtcc
     2041 cgagtgaaat tcaccggttt tttcccgagc tggagcgtta tagagcagca cctcgccgcg
     2101 ttcatttccg actgcaagtg tttcaccatc gggtgagaag gccagtgaaa agacgtttcc
     2161 cacgtcgcct ggaagctcgc ccacaaccga gccgtcgctg gcgctcagaa tccgcgcgcc
     2221 cccttcgcgt gcgccgaccg ccgtgctgaa aaagcgatcc ggaggactgc catgaccttg
     2281 gatcagcttg gcggttccga ccgccagcca cttcccatct gccgaaagag ccagtgccaa
     2341 aggcgggtcg gggctggcaa cttcccaaag gacctcgccc gtggcggcgc tgaatagctt
     2401 gacttcagac gttccgttaa gctgcccgct gcagattgcc agtcgcgcgc tatcggagga
     2461 ccacgccacg gccgtgacgc aggccttttg caacggcagc gtccggagtg gctcgcgagt
     2521 gcgggcgtca tagaggatca cctcgccctg gttcgaccgg ccgccggcta ccgccaacat
     2581 ggtgccgtcc ggcgacacct tggcggaccg cacctcgcgg accgcgcctg ctttctcttg
     2641 gtcccacaat atccctgcag ccgaggaatg tgccagcgac cacaggtaat accactcgaa
     2701 gccgcggagg tcttcatgtc cctcggcggg gatgtggtta tcgagcaggt cgatggcttg
     2761 tgcgacatcg ccggcggcga gcacttgctg cgccaggttc acgtcggcga agtactgatt
     2821 cttccgggcg aacgcggcgc tttccgtggc cgcaatggca ctcttcttcg cctgttcggc
     2881 tgcggtggta gccagcacgg cgtttttggt tgccaggcgc tcctgctcgc gccatttacg
     2941 ggcgttttcc tgggccttgc ggttgttggc gtcggcaatt cccttttgct ggaccgcgtc
     3001 cgctttctga taattggcgt aaatcgccag cgtagtcgaa acggccatgc cgcccaccag
     3061 caagaggacc gccagcccca tcaatccggc gaccattcga ttacggttgc accaacgaac
     3121 ggctcgctcg acgcggccaa caggccgggc caggatcggc tcgccgcgca gataatgcgc
     3181 gaggtcatcg gccagcgcgg cagccgtcgg atag
//
LOCUS       DASUGB010000002         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_139161, whole genome shotgun sequence.
ACCESSION   DASUGB010000002 DASUGB010000000
VERSION     DASUGB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139161"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFV87_00010"
     CDS             <1..562
                     /locus_tag="VFV87_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102161.1"
                     /translation="TVLIASVVGPEAATGGVPAQPLRMGGSHAVGIVHPAVWVVAGVS
                     FLAAAILVLMNLLIFAAAAVGVAVVAGIVGLTQRYRGYAAGTELGAGRMLGRGPYTST
                     VCPPSHELVQKLADTLDELREAARDGDWTIDWHPLDDCCRAAVSADRSGHFAEATRQY
                     CRGISYVMKALRSQPGKRAGDSAVKY"
     gene            complement(621..2261)
                     /locus_tag="VFV87_00015"
     CDS             complement(621..2261)
                     /locus_tag="VFV87_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102162.1"
                     /translation="MAIHCPYCRHKMALKDAKPGKYKPKCAKCGTRFLLTVPADGAEP
                     VSEAIIQELRLPEGVVDPAAAVRSREDAQMLEECATIFNRAGMAKLRTPLVLGFLGLV
                     AVLSCGGLIWSWALVAGTLALAAAAIGTYFVASGLRQRTFLFGKFRELVWMARWTDWA
                     TWIVSGILLIVILLVLGWLWMALGMAIAGMLLGLAHCTVVDGRIAAQRRQPLEEMEHL
                     LKTLRLKGIDETALQQFVAKYSGQQWEEFFEALFGYEDKLTARKLWGRGEGGRDRPKF
                     CGWQDPIVCWIDRRVQAFRELRDRKHLQQVEEKNLQAQGLDLITARRRAQNMADAILD
                     EAAEARAAAAQPAAAQAVIDPKLEAAKKRQRIKTMLADAKKGAYQAKRQRMIGTALSP
                     LAIALGGGVRFFLGCLLVIGCVLWARQNGLLSRENVHQVGSAVSASVQHGDAASLAAD
                     AQSLTSRDLRPLRIPVVGPLVSSFNAGIAGLVLIVLGLFRGWKMSLFALPATAVMLLG
                     PAVHVPAFLSLQTVHLESIGAGLLIAAVGVVFGRTHDD"
     gene            complement(2375..>2638)
                     /locus_tag="VFV87_00020"
     CDS             complement(2375..>2638)
                     /locus_tag="VFV87_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102163.1"
                     /translation="DAKISVDVADLGFLRVGDKIEVRGWYPAGTKGRAIANQVTASAA
                     EPLADPSRKKKPAAVDPAASDNEPKSEKPAAGESKPEKEASGE"
BASE COUNT          472 a          872 c          817 g          477 t
ORIGIN      
        1 caccgtgctc attgccagcg tcgtcggccc cgaagccgcc acgggcggcg ttcccgccca
       61 gccgctgcgg atgggcgggt cgcatgccgt tggcatcgtg catcccgccg tatgggtcgt
      121 ggccggcgtc agctttttgg ccgccgcgat tctcgtactg atgaacctgc tcatctttgc
      181 ggctgcagcg gtaggtgtag cggtcgttgc gggcattgtc ggtctgacgc aacgttatcg
      241 cgggtatgcg gcgggcaccg agctcggcgc cgggcgcatg ctgggtcgtg ggccctatac
      301 ctcgacggtc tgcccgccca gccatgaact cgtgcaaaag ctagccgata cgctcgacga
      361 gctgcgtgag gcggcgcgag acggggactg gacgatcgat tggcatccgc tcgacgattg
      421 ttgtcgggcc gccgtgagcg ccgatcggtc tggccacttt gccgaggcca cacgtcaata
      481 ctgccgcggc atcagctacg tcatgaaggc cctccgctcg cagcctggca aacgcgccgg
      541 cgactccgcc gtgaagtatt aggcccgcgc gtttcatccg aagttttggc gacattgcaa
      601 aagggaatga ttacgagctg ctaatcgtca tgagtccgcc cgaagacgac gccgaccgcg
      661 gcgatcaaga gtcccgcgcc aatactctcc aaatgaaccg tctgcagcga taaaaacgcc
      721 ggcacgtgaa cggccggccc caagagcatc acggcggtgg cgggcagggc gaagagcgac
      781 atcttccaac cccggaagag gcccagcacg atcagcacca gccccgcgat cccggcattg
      841 aagctgctga ccagcgggcc gaccacagga attcgtaggg gccttaggtc gcggctggtc
      901 aacgactgcg cgtccgcggc caggctggcg gcgtccccat gctgcacgct ggccgagaca
      961 gccgagccga cctggtgcac gttttcacga gataacaggc cattctgccg ggcccagagg
     1021 acacagccga tcacaagcag gcagccaaga aaaaaccgta ccccgccccc caaagcaatg
     1081 gcgagcggcg aaagagccgt gccgatcatc cgctggcgtt tggcctggta tgctcccttt
     1141 ttcgcgtcgg ccagcatcgt cttgatccgc tgccgctttt ttgcagcttc gagctttgga
     1201 tcaatgaccg cctgcgctgc cgccggttgg gcggctgcgg cgcgggcctc ggctgcttcg
     1261 tcgagaattg cgtcggccat gttttgagcc cggcggcggg cggtgatgag atccagcccc
     1321 tgggcctgca ggtttttctc ctcgacttgc tggaggtgct tgcggtcgcg cagttcccga
     1381 aatgcctgca cgcggcggtc gatccagcag acgatcgggt cttgccagcc gcagaacttg
     1441 ggccggtcgc ggcctccttc gccgcggccc cacaacttgc gggccgtcag cttgtcctca
     1501 tagccgaaga gggcctcgaa gaactcctcc cactgctggc cgctgtactt ggcaacgaac
     1561 tgctggaggg ccgtttcgtc aatccccttc agccgcaatg ttttgaggag atgctccatc
     1621 tcttccagtg gctggcggcg ctgggccgca atgcggccat cgaccacagt gcaatgcgcc
     1681 aggcccagga gcatgccggc gatcgccatc cccagtgcca tccacagcca cccgaggacg
     1741 agcagaatga cgattagcag gatgccactg acgatccacg tcgcccagtc ggtccaacga
     1801 gccatccata ccagctcgcg gaattttccg aagaggaacg tccgctggcg caagccgctg
     1861 gcgacaaagt aggttccaat cgccgcggcg gccagggcca aagtgccggc gaccagcgcc
     1921 caactccaga tgagaccgcc gcagctcaag accgcgacca gccccagaaa ccccagcacc
     1981 agcggcgtgc ggagcttcgc catgccggcg cggttgaaga tggtggcgca ttcctccagc
     2041 atttgggcat cctctctgct ccgcacggcg gcggcgggat caactacccc ttcgggcaaa
     2101 cgcaattcct gaataattgc ttcgctcacg ggctcggcgc catctgcagg cacggtcagc
     2161 agaaaccgcg tgccgcattt ggcgcacttg ggcttgtact tgcccggctt ggcgtccttg
     2221 agcgccatct tgtgacggca ataaggacaa tgaatcgcca tcgaagtcgc cgccccagga
     2281 tgcaagacgc atcataatcg ggggagatcg caacgtggaa tgcgcggcac gctcggcgca
     2341 aaaacttcac attcctgccg tagcaaagta tcggctactc gccggacgcc tctttctctg
     2401 gcttgctctc gcccgcggca ggcttctcgg atttcggctc gttgtcgctg gcagccggat
     2461 caacggcggc aggtttcttt ttcctgcttg gatcggccag cggctcggcg gcggaggcgg
     2521 tgacctgatt ggcgatggcc cggcccttgg tgccggccgg ataccagccg cggacctcga
     2581 tcttgtcgcc cactcgcagg aatcccagat cagccacatc gaccgaaatc tttgcgtc
//
LOCUS       DASUGB010000003         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1389458, whole genome shotgun sequence.
ACCESSION   DASUGB010000003 DASUGB010000000
VERSION     DASUGB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389458"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFV87_00025"
     CDS             <1..388
                     /locus_tag="VFV87_00025"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX5102164.1"
                     /translation="YSRYPWRKDGGPKDEFEQRALDWLERYPDDPYREFTSVGGQRSL
                     VYATARKMELSCVKCHNASDRSPKKDWKVGDVVGVLKIVRPLDRDIARTQVGLRGAFL
                     TMGGIAALLLAVSVGVLIGARSRGRA"
     gene            479..1099
                     /locus_tag="VFV87_00030"
     CDS             479..1099
                     /locus_tag="VFV87_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5102165.1"
                     /translation="MQHKPSSNGQASGGSTSRSLLSRLKTSDAAAWNRLVVLYAPLVH
                     HWCRQFGLPTREAADVLQDVFQAVAANIERYHRDAPGDSFRGWLRAITRSKVMDHFRR
                     RSSQPEAAGGTEALQRLGQLPAPADFMDGDSLAADQAPVRALFESALSLIRPQFEDRT
                     WQAFWRTAVEDQPAADVAAELSMSPGAVRVAKCRVLQRLREELGEM"
     gene            1892..>2085
                     /locus_tag="VFV87_00035"
     CDS             1892..>2085
                     /locus_tag="VFV87_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102166.1"
                     /translation="MPTLSRRFLALGLAVVALGWCQPLVAVDKTAAELLPGSVVAYAE
                     IPHPGKILDTVLDHPLAAEI"
BASE COUNT          343 a          637 c          723 g          382 t
ORIGIN      
        1 ctatagccgc tatccgtggc ggaaggacgg cgggccaaag gacgaattcg agcagcgggc
       61 tcttgattgg ctggaacggt atccggacga tccctaccgc gaattcacct cggtcggcgg
      121 acagcggtcg ctggtctacg cgacggctcg caagatggag ctgagctgcg tcaagtgcca
      181 caatgcttcg gaccgcagcc ccaagaagga ctggaaggtc ggtgacgtcg tgggcgtgct
      241 caagatcgtg cggccgctcg atcgtgacat cgcccgcacg caggtcggcc tccgcggcgc
      301 atttttgacg atgggcggca tcgcggcgct gctcttggcc gtctcggttg gcgtgctgat
      361 tggcgcccgt agccgcggcc gtgcgtgaag tggggcaggc attcttgcct gccgaggaac
      421 cgcgaccact ggtcgcggct ttggaatggg gtcggcgcga cgcctgcccc acgaaaacat
      481 gcagcacaag ccatccagca acggacaagc cagcggcggc tcgacgtcgc gcagcctgct
      541 cagccggctg aaaacgtccg atgcggcagc gtggaaccga ttggtggtgc tgtacgctcc
      601 gctggtacac cactggtgcc gacagttcgg cctgccgact cgggaagcgg ccgacgtgtt
      661 gcaggatgtg tttcaagcgg tcgccgccaa catcgaacgt tatcaccgcg atgccccggg
      721 cgattcgttc cgcggctggc tgcgggcgat cacgaggagc aaggtgatgg accacttccg
      781 ccgccgctcg tcgcagcccg aagccgccgg cggcaccgaa gccctgcagc gactggggca
      841 attgccggct ccggcggatt ttatggatgg cgatagtctc gcggcggacc aagcccccgt
      901 gcgggcactc ttcgaaagcg ccctcagcct gattcgcccc cagttcgagg accgcacctg
      961 gcaggccttc tggcggaccg ccgtcgagga tcaacccgcc gcggacgtgg ccgcagagct
     1021 ttcgatgagc cccggggctg tccgggtcgc caagtgccgt gtgctgcaaa ggctgcgtga
     1081 agaattgggc gagatgtaag ggtttccgcc tgacgcgtag cggcgacgat agcgtggccg
     1141 aagggcgtag ggtgggacaa ggccgccagg ccgtaggccc accattcacg agcgttggcc
     1201 ctggtcggcc tgcgctcgct ccgctcgctg gtcgcaccct gcaacggcct gtcaccttca
     1261 ggaattcgtt cttgcgggcc ctttcatgcg ccgcttggta gaatctcgga catgaacgcc
     1321 cgatcgtcac cttggcctga gcatcgactt gctgagattc aggcaatgct ccgcgagcgt
     1381 caagaccgag ctgagattga gagtctgatg cggcgagcgg cgacggaatt gggcgatgat
     1441 gccgaggccg cggttaccca gtggagagag tgggcatcac aggaaggcga attgtggtca
     1501 tattgctctg actcaaggtc gtgggatcgc atggccggtg aatgcggatg ggccattgtg
     1561 cagcatggca aggtcgtgga cttcaaaatt atggcggtga attagctgct gaggccaccg
     1621 cggagcggtg ggtgatggag ccgcgtcgcc aacgtgagcc gcgtagcggc gatacctgcg
     1681 ccacggacgg tttcgccgct ccgcgactgg aaggagatcg gtggctcgcg gtcccacagc
     1741 tacgctgtgg gctcagggct ggcgctgctc cgtagcgggg gacgcttgaa gctcgtccgg
     1801 cgcgtgccgg tgaaactccg ctctgctatc cggggtacgt ttggtgcaag tcgggttcgg
     1861 cggacctggt ccctactctc tggagtcccg tatgcccacg ctttctcgcc gctttctcgc
     1921 gcttggcctg gcggtcgttg ccctcggttg gtgtcagccg cttgtcgcgg tggacaagac
     1981 ggcggctgag ctgctgcccg gctcggtcgt ggcgtatgcc gaaatccccc acccggggaa
     2041 gatcctggac acggtcctcg atcacccgct ggcggcggaa atcca
//
LOCUS       DASUGB010000004         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1111728, whole genome shotgun sequence.
ACCESSION   DASUGB010000004 DASUGB010000000
VERSION     DASUGB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111728"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFV87_00040"
     CDS             <1..434
                     /locus_tag="VFV87_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5102167.1"
                     /translation="MRWLAPIIEPLWLAVDRRLPLGRRGENAAARHLQRLGYVIVARG
                     HRGLIGEIDLVAVEGRTLVFVEVKTRTSQDAGHPADAVDAAKQRRLTRLALSYMKRHD
                     LLDNPARFDVIAVTWPDQGSRPVIEHFKDAFEPVGFEGMFS"
     gene            548..676
                     /locus_tag="VFV87_00045"
     CDS             548..676
                     /locus_tag="VFV87_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102168.1"
                     /translation="MDNLQLAGLIERFVRQREPAARLEAELVDFHSSRPGHDLAEL"
     gene            930..>2357
                     /locus_tag="VFV87_00050"
     CDS             930..>2357
                     /locus_tag="VFV87_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102169.1"
                     /translation="MSSAQEASTAPAQAVAAQAAGAAGEAAEEKRIAELIQRLGLEDF
                     GEREKAQAELAQLGLAAFDALHTAQNHNDPEIALRARYLVRSMNVRWFQESDSPKVVL
                     ALKNYGEFSEPERKSRMDLLATLPQGEGIPALCRLSRFETVETLSKYAALKILQQPLP
                     TETATKEQIRETIASIVGHSKRPAANWMRLYSQTLTDPAAALAQWQQTAADETLTMER
                     NPHQSSRDIVRDLYRFQVELLKGLKRDDEAVAVMRRTFDLLDDTTEQVTEVTDWLLQR
                     QAWPVVLELYQQKAKTFEDNALLLYRLAETHKKLGDAQQAQEFADRALALKPENPEEH
                     IRTGYVLQEERGLIDWAEREYREVIKNSPAGAVSDFKARFRLSELLHDQGGELSSAEA
                     LQPVVDLMAKDEAARQTCARALRDPNGVISRMNYFYAMHFHEQNDAAKERQHLQAAID
                     ADPTDADVLIGMYRLPGADDAWKKMT"
BASE COUNT          445 a          731 c          786 g          395 t
ORIGIN      
        1 ccatgcgctg gctggccccc atcattgaac cgctgtggct ggctgtggat cgtcggctgc
       61 cgctgggaag gcgaggggag aatgcggcgg cgcggcatct gcagcgtctg ggttacgtga
      121 tcgtggcccg cgggcaccgc ggactgattg gcgagatcga tctggtcgcg gttgaagggc
      181 ggacgctggt gtttgtggaa gtgaagaccc gcacctcgca agatgctggc catcctgccg
      241 acgccgtcga tgccgccaag cagcggcgac tgacgcggct ggcgctctcg tacatgaagc
      301 ggcacgattt gcttgataac ccggcccggt tcgacgtgat cgcggtcacc tggcccgacc
      361 agggcagccg gcccgtcatc gagcacttca aggacgcctt cgagccggtg gggtttgaag
      421 gaatgttctc gtagccgagg aagaatcgcc tgccaagcca tggtcgcggc tcgtatcgac
      481 ccagccgcac agcgtgacct cctgaccgac ctttcgggag aagcacaaca tcgtcggcga
      541 cgtggaggtg gataacctcc agcttgccgg cttgatcgaa cggttcgtcc ggcagcgcga
      601 gcccgccgcc cgcctggagg ctgagctcgt cgatttccac tccagccgcc ccggccacga
      661 cctggccgaa ctataaggcg gcgattccgg aatgacttcc gtcatctgcg tccagatcta
      721 ctctgtctta acttgcggat ggtcctcgac agccttgttt gcgcaaagcc ggagttgccc
      781 tgtccggagg cgccggggtg gggtaagctg aaactgggtc gttgattcga gggcatgtgc
      841 gaggcaacac catggagtcc atccgattcg cggttacgca gttgctcgca ggcgtatgca
      901 catggtctgc ctcgggactc gctgttgctg tgagcagcgc ccaggaggca agtactgcgc
      961 cagcgcaggc ggtggcggcg caggccgccg gagccgccgg cgaggccgcg gaggagaagc
     1021 ggattgcgga gttgatccag cggctggggc tggaggattt tggcgaacgc gagaaggccc
     1081 aggctgagct ggcgcagctc ggcctggcgg cgttcgacgc cctgcacacc gcccagaacc
     1141 acaacgatcc ggaaatcgcc ctgcgggccc gctacctggt ccgcagcatg aacgtccgct
     1201 ggttccagga gtcggattcg cccaaggtcg tgctggctct gaagaactat ggcgaattct
     1261 ccgagcccga gcggaaatcg cggatggatt tgctggccac gctgccgcag ggcgaaggca
     1321 ttccggcctt gtgccgcctc tcgcgattcg aaacggtcga aacactctcc aagtacgcgg
     1381 ccctgaagat cctccagcag ccgctgccca cagaaacagc gacgaaggag cagattcgcg
     1441 agacaatcgc ctcgatcgtc ggccatagca agcggcccgc ggccaactgg atgcggctgt
     1501 actcccagac gctcaccgat ccggcggcgg ccctcgccca atggcagcaa acggcggccg
     1561 atgagacgct gaccatggag cgcaatccgc atcagtcgag ccgcgacatc gtccgcgatc
     1621 tgtaccgctt ccaggtcgag ctgctcaagg ggctgaagcg cgacgacgaa gcggtcgccg
     1681 tgatgcgccg cacgttcgac ctgctcgacg acaccaccga gcaggtgacc gaagtgaccg
     1741 attggctcct gcagcggcag gcgtggccgg ttgtgctgga gctgtatcag caaaaggcca
     1801 agaccttcga ggacaacgct ctgttgctct atcggctggc cgaaacgcac aagaagctgg
     1861 gcgacgccca acaggcccag gaattcgccg atcgggccct ggccctaaag cccgagaacc
     1921 cggaagagca catccgcacc ggctacgtcc tgcaggagga gcgcgggttg atcgattggg
     1981 ccgagcgcga gtaccgtgag gtcatcaaga attcgcctgc cggggcggtc tctgatttca
     2041 aggcccgctt ccggctgtcg gaattgctgc acgaccaggg gggcgagctg tcgtctgcag
     2101 aggccctgca gccggtggtc gatctgatgg ccaaggacga agcggccagg cagacgtgtg
     2161 cccgcgcatt gcgtgatccc aacggcgtga tttcgcggat gaattacttc tacgccatgc
     2221 actttcacga gcagaacgac gcggccaaag agcgtcagca cctacaggcg gcgatcgacg
     2281 ccgatccgac cgatgcggac gtgctgatcg ggatgtatcg cctgccaggc gcggacgacg
     2341 cttggaagaa aatgacg
//
LOCUS       DASUGB010000005         5235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1806578, whole genome shotgun sequence.
ACCESSION   DASUGB010000005 DASUGB010000000
VERSION     DASUGB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806578"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="VFV87_00055"
     CDS             <1..1957
                     /locus_tag="VFV87_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX5102170.1"
                     /translation="SKIPSTGYQPRLADDRVGYFLTAVKDFSSKSDREQFVRYINRWD
                     LQKADSSADPSPPKKPIVFHIEKTVPFKYRKAIYDGIYEWNKAFEKAGFVNAVEVHQQ
                     LDNDDKDPEDIRYNFFRWITSNAGFAMGPSRVNPYTGQILDADIIFDADFLTSWKEEY
                     ETFTAATIAEMTGGALDIHNEAEPQPRSIFLPREPSGADCRRAQGMSMQLAFGHTAIL
                     ANNDPKVVEENLDKLIQQGLKEVTMHEVGHTLGLRHNFKASTIWSLKDLNDPEKCKGC
                     LVASVMDYSPTNIVPKDWKQGDYYSTTIGPYDYWAIEYGYKALSGGTSGELTDLKKIA
                     SRSGEPQFAYATDEDTRGIDPDPDSNRFDLGSDPLEYAKTRLQLVKELLPDLVEKTTK
                     EGDDYTQARRAFNIVVSQYAQSTFFASRYVGGLKTSRSHKGDKDGKDPIELVDVKLQR
                     EALGMLEENIFSDQNFQFPPELLTKLGWSNWSHWGTRGTTRKDFGVHDFVLQWQERVL
                     AQLLSSVTLSRMHDAELKATTDADVLTTAELIQRLTKAIFSEVETTKEGDYSLRKPAI
                     SSLRRNLQRSYLKQISHMALGQTGAPQDCQTIAWAELSNLNQRMKSLLDNQPVAGKLD
                     AYSRAHLQESQARIQKVLEAKLSLNSP"
     gene            2172..3728
                     /locus_tag="VFV87_00060"
     CDS             2172..3728
                     /locus_tag="VFV87_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX5102171.1"
                     /translation="MPVTAGRVTDAVYLSPDDNICVAARNLEAGTLLKIADTEFRLAG
                     PVKLGHKIAIRPIKKGDFVRKFGQIIGQTTASVEPGGWVHSHNLVNGEFARDHAPATA
                     IPPSPAPIEGRTFMGYKRASGKAGTRNYIAVISTVNCSATVSRHIAERFDKQILKQFP
                     NVDGIIAIKHGGGCGMQYQGLQHEILNRTLAGMARHPNIGGYLLVGLGCEQGAMGYLL
                     QSQQLVQIDGGNGKPNAPPVLSMQDEGGTLKTIEAGCRTLMDLLPRANDVKREPLPAS
                     EILLGTNCGGSDGNSGVTCNPALGIASDLLVACGGTSALAETTEAYGAEHLLTRRAVS
                     KDVANKLLDRIKWWLWHTSLYGVEINNNPSVGNKEGGLTTIAEKSLGAIAKAGSTAMT
                     EVYEYAEPIMKKGFVFMDTPGFDPPSVTGLVAGGCNVMVFTTGRGSCFGLKPCPTIKV
                     ASNTPMYERMRDDMDINAGEILAGRSVEDVGREIFEKILAVASGEKTKSEQHGYGDEE
                     FVPWQVGPML"
     gene            complement(3864..4973)
                     /gene="tgt"
                     /locus_tag="VFV87_00065"
     CDS             complement(3864..4973)
                     /gene="tgt"
                     /locus_tag="VFV87_00065"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5102172.1"
                     /translation="MSFRFDLLKSDPNSSARRSTFHTPHGAVELPAFMPVGTQGAIKG
                     VTVDQLRATGARMILANTYHLALRPGEEVIRQLGGLHRLMGWDGPILTDSGGFQLFSL
                     AQNTKVTEQAAIFRSHIDGKLLELSPERAIAIQEALGSDVAMVLDHVLPLPAADDAVR
                     DACDRTVRWAERCQQAATRSDQAQFAIVQGGLNPELRLWCARQLADLDFSGYAVGGLS
                     VGESPAEMYAVLDVVSPALPREKPRYLMGVGTPQDLLEAIRRGIDMFDCVLPTRNGRN
                     ALAFTDRGPMRLRNQVHQTDERPLEENCPCPACRHSRAYLRHLFLADEMLGPILLSIH
                     NLTYYQRLMRETRQAIEQDRFTAFMDEKLRGWSAA"
BASE COUNT         1082 a         1699 c         1616 g          838 t
ORIGIN      
        1 cagcaagatt cccagcaccg gctatcagcc gcgattggcc gacgaccgcg tggggtattt
       61 cctcacggcg gtgaaagact tttcgagcaa gagcgatcgc gagcagttcg tccgctacat
      121 caaccgctgg gacttgcaga aggccgactc ctcggccgat ccctcgccgc cgaagaagcc
      181 gatcgtgttc cacatcgaga agaccgtgcc gttcaagtac cgcaaggcga tctacgacgg
      241 catctacgag tggaacaagg ccttcgagaa ggcgggtttc gtcaacgcgg tcgaggtcca
      301 ccagcagctc gacaacgacg acaaggaccc cgaggacatt cgctacaact ttttccgctg
      361 gattaccagc aacgcaggtt ttgcgatggg ccccagccgc gtgaatccgt acacggggca
      421 gattctcgac gccgacatca ttttcgacgc cgatttcctc acctcctgga aggaagagta
      481 cgagaccttc accgccgcca cgatcgccga aatgaccggc ggcgcgctcg acatccacaa
      541 cgaagccgag ccgcagccgc ggagcatctt cttgccccgc gagccgagcg gcgccgactg
      601 ccggcgggcc cagggcatga gcatgcagct cgcgttcggc catacggcga ttctggcgaa
      661 caacgatccc aaggtcgtgg aggaaaacct cgacaagctg atccagcagg ggctcaagga
      721 agtgacgatg cacgaagtcg ggcacacgct cggcctgcgg cacaacttca aggccagcac
      781 gatctggagc ttgaaggact tgaatgaccc cgagaagtgc aaaggctgcc tggtcgcctc
      841 cgtaatggac tacagcccga cgaatatcgt tcccaaggac tggaagcagg gcgattatta
      901 cagcacgacg atcggcccgt acgactactg ggccatcgaa tatggctata aagccctgtc
      961 cggcgggacg agcggcgagc tgaccgacct gaagaaaatc gcctcgcgca gcggcgagcc
     1021 gcaattcgcc tatgccaccg acgaagacac ccgcggcatc gatcccgatc cggacagcaa
     1081 ccggttcgac ctgggaagcg atccgctgga atatgccaag actcggttgc agctcgtcaa
     1141 ggagctgctg ccggacctgg tggaaaagac cacgaaggaa ggcgacgact acacgcaggc
     1201 ccgccgggcg ttcaacattg tcgtcagcca atacgcccag agcacgttct tcgcctcccg
     1261 ctacgtcggc ggcctgaaaa cgtcgcgcag ccacaagggc gataaggacg gcaaggaccc
     1321 gatcgagctg gtcgatgtca agctgcagcg cgaagcgctg ggaatgctcg aagaaaatat
     1381 cttcagcgat cagaacttcc agttcccgcc ggaattgctc accaagctcg gctggtcgaa
     1441 ctggagccac tggggaacgc gcgggacgac gcgcaaggac ttcggcgtcc acgacttcgt
     1501 gctgcaatgg caggagcgcg tgctagccca gttgctctcg tcggtcacgc tcagccgcat
     1561 gcacgacgcc gagctcaagg cgaccacgga cgccgacgtg ctgacgaccg ccgagctgat
     1621 ccagcggctg acgaaggcga ttttctcgga ggtcgagacg accaaggagg gagactattc
     1681 gctccgcaag ccggccatca gcagcctgcg tcggaacctg cagcgctcgt acctgaagca
     1741 gatttcgcac atggccctgg ggcagaccgg cgccccgcag gattgccaga cgatcgcctg
     1801 ggccgagctg tcgaacctca accagcggat gaagagcctg ctcgacaacc agccggtcgc
     1861 cggcaagctc gacgcctaca gtcgggccca cctgcaggaa tcgcaggccc gcatccagaa
     1921 agtgctggaa gcgaagttga gcctgaattc accgtaaacc ttgtcgttta acggcgagcc
     1981 ccaggcgatg ccgtgtaggg tgggaccagc gcagcgcagg cccaccatcc ggcgattcgc
     2041 agggcgcgca gccggagtat aatcaggctg tcgccagcca acgtactgag tactaagtac
     2101 tcagtaccaa gtatcttggt cgccagtgcg ccgcgggccg gccagcgcac ccttaggtat
     2161 cgctccgcca catgcccgtc accgccggcc gcgtcaccga cgccgtttat ctctcgcccg
     2221 acgacaatat ctgcgtcgcc gctcgcaacc tggaagcagg cacgctgctg aagatcgcgg
     2281 acacggaatt ccgcctggca gggccggtca agctcggcca caagatcgcc atccggccga
     2341 tcaaaaaggg agacttcgtc cgcaagttcg gccagatcat cggccagacg acggccagcg
     2401 tcgagcctgg cggctgggtg cattcgcaca acctggtgaa cggtgaattc gcccgcgatc
     2461 atgccccggc gactgcgatc ccgccgtcgc cggcgccgat cgaaggccgg acgttcatgg
     2521 gctacaagcg ggcgagcggc aaagcgggga cgcggaacta catagccgtc atctccactg
     2581 tgaactgctc ggccaccgtc agccggcata tcgcggagag gttcgacaag cagattctga
     2641 agcagttccc gaacgtcgat gggatcattg cgatcaagca cggcggcggc tgcgggatgc
     2701 agtaccaggg actgcagcac gagatcctga accggacgct tgccgggatg gcccgccatc
     2761 caaacatcgg cggctacctg ctcgtcggcc tcggctgcga gcaaggcgcg atgggctacc
     2821 tccttcaatc gcaacagctc gtgcagatcg acggtggcaa cggcaaaccc aatgcaccgc
     2881 ccgtgctctc gatgcaggac gaaggcggca cgctcaaaac catcgaagcc ggctgccgga
     2941 cgctgatgga cctcttgccg cgggccaatg acgtgaagcg cgagccgctc ccggcgagcg
     3001 agatcctgct gggcaccaac tgcggcggtt ccgacggcaa cagcggcgtg acgtgcaacc
     3061 cggccctggg catcgccagc gacctgctcg tggcctgcgg cggcacttcc gccctggccg
     3121 aaacgaccga agcctacggc gcggagcatc tgctgacccg ccgggccgtc agcaaggacg
     3181 tcgccaacaa gctcctggac cggatcaaat ggtggctctg gcataccagt ctctacggcg
     3241 tcgagatcaa caacaacccc agcgtcggca acaaggaagg cggcctgacc acgatcgccg
     3301 aaaaatcgct cggggccatc gccaaagccg gcagcaccgc catgaccgag gtctatgaat
     3361 acgccgagcc gatcatgaag aagggcttcg tcttcatgga tacgcccggc ttcgacccgc
     3421 cgagcgtgac gggcctcgtg gccggcggct gcaacgtgat ggtcttcacc accggccgcg
     3481 gaagctgctt cggcctcaag ccctgcccga cgatcaaagt tgccagcaac acgccgatgt
     3541 acgagcggat gcgtgacgac atggacatca acgccggcga aatcctcgcc ggccgaagtg
     3601 tggaagacgt cggccgcgaa atcttcgaaa agatcctcgc cgtcgccagc ggcgaaaaaa
     3661 ccaaaagcga acaacacggc tacggcgacg aggaattcgt cccatggcaa gtcgggccga
     3721 tgctgtagta gtcttctcgc tccgcgagaa gcaagacgtt ttccaccagc ataaccgcgg
     3781 cgcgcaaaat ggccgtactg cctctcgcgg agcgagatgc ctatgatggt gggcctgcgc
     3841 tcgcttcgct cgctggtccc accctacgcg gcgctccagc cgcggagttt ctcgtccatg
     3901 aacgcggtaa agcggtcctg ctcgatcgcc tgccgggtct cccgcatcag ccgctggtag
     3961 taggtcaggt tgtggatcga aagcaggatc gggccgagca tttcgtcggc tagaaacagg
     4021 tgccgcagat aagcccggct gtggcggcag gcggggcagg ggcagttctc ttccagcggc
     4081 cgctcgtcgg tctggtggac ctggttccgc agccgcatgg ggccgcggtc ggtgaaggcc
     4141 agggcgttgc ggccgttgcg ggtcggcagg acgcagtcga acatgtcgat tccgcggcgg
     4201 atcgcctcca gcaagtcctg cggcgtgccg acgcccatca aataccgcgg cttctcgcgg
     4261 ggcagggctg gcgagacgac gtcgagcacg gcgtacatct cggccggcga ttcaccgacc
     4321 gagaggccgc caaccgcata tcccgaaaaa tcgaggtcgg caagctgccg ggcacaccac
     4381 agccgcagct cggggttgag gcccccctgg acgatggcga actgggcctg atcgctccgc
     4441 gtagccgcct gttggcaccg ctcggcccag cggaccgtcc ggtcgcaggc gtcacggacc
     4501 gcatcgtccg cggccggcag cggcagcacg tggtcgagca ccatcgccac atcgctgccc
     4561 aaggcttcct gaatggcgat cgcccgctcg gggctcagct ccagcagttt gccgtcgatg
     4621 tgcgagcgga aaatggccgc ctgctcggta accttcgtgt tctgcgccaa actgaagagc
     4681 tgaaacccgc cgctgtcggt gaggatcggc ccatcccagc ccataaggcg gtgcaagccg
     4741 ccgagctggc ggatgacctc ttcgccaggc cgcagggcga ggtgataggt gtttgccagg
     4801 atcatccgcg cgccggtggc ccggagctga tcaaccgtca cccccttgat cgccccctgc
     4861 gtgccgaccg gcatgaacgc gggcaattcg acggcgccgt gcggcgtgtg aaacgtgctg
     4921 cggcgggcgg aggaatttgg atcagacttc agcaggtcga agcggaagga cattggctac
     4981 atcaaacgcc ggcagttgaa aattggtaat tgcaaattgc aaatttcaaa tctgaatttc
     5041 gcaattttca ttttgcaatt ttcaattgcc ggatgccaat tgcttgttcg gctcctgtcc
     5101 tctgtcttct gtcctcttgc caagacgggc aggcgagacg ccgaccccac gtcagtcccc
     5161 ccgcagcttc agccccatcg ccgtcagctt ctcgcggact tcgttcaggc tcgtgacgcc
     5221 gaagttcttg cactc
//
LOCUS       DASUGB010000006         5413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1251205, whole genome shotgun sequence.
ACCESSION   DASUGB010000006 DASUGB010000000
VERSION     DASUGB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251205"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VFV87_00070"
     CDS             <1..1411
                     /locus_tag="VFV87_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102173.1"
                     /translation="GSGRLELAQAIVDSANPLTARVIVNRVWAQHFGAGLVRTPSDFG
                     TRAEAPSHPELLDWLVSRFIEDGWSLKKLHRRIMMSQTYRQASQETEDRGQRTEAEGA
                     NTDVENPKSQIPNPKSTDPQNRLLWRMNERRLSFEELRDSLLAASGKLDCTAGGKAED
                     LFSPQFVRRSLYGTIDRQFLPSTLRMFDFANPDLHIPQRSETTVPQQALFFLNHPLAV
                     GYAQALAARTTAAATDEQRIQQLYQFAYQRLPTPEQTQAALEFIRLAQGDTTGAIPPT
                     VNAWQYGFGAYDEKMQRASSFEKLPHFTGSAWQGGPAWSDAKLGWAQLTAAGGHPGND
                     LAHAAIRRWTAPRDMPIRIESLLVHEPAEGEGVRGFLICSRGGLLHEAKVHHGQAEFN
                     AARLDVTAGDTLDFVADIGNKLSHNQFLWRATITPLDDSGPPFDSVRDFENQGKTRLT
                     PWEQLAQVLLSANELMFVD"
     gene            complement(1442..2617)
                     /locus_tag="VFV87_00075"
     CDS             complement(1442..2617)
                     /locus_tag="VFV87_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5102174.1"
                     /translation="MNRFKYWLLSFWITALHLAPSLAAGDEVRVASRDELVRAFGNAQ
                     PGTTILIAPGTYRGGIAPGKLQGRKDQPIVIAAADPADPPVIEGGGSGLHLSSPAHLE
                     LRDLIIAKANGNGINIDDGGSSQSPGHDLFLRNLTIRDVGPSGNRDGLKLSGVNDFRV
                     DGCRIMRWGSNGSAIDMVGCQRGLVTSCTFQEGGDQANGVQAKGGSNQIVVQYCRFEN
                     AGGRAINIGGSTGLDYFRPQLGDFEAKEITVQDCEIIGGMSAIAFVGCDGALVQHNTI
                     YRPTRWPLRILQENTDAKFVPSRKGVFQKNLVVFRTGEVRQVVNIGPGTAPETFEFTG
                     NQWHALDAPNQTQRFLRLPAKETSGIYNHPPEFKDADQLDFSLPGRKPSDPGVRPAT"
     gene            complement(2675..4123)
                     /locus_tag="VFV87_00080"
     CDS             complement(2675..4123)
                     /locus_tag="VFV87_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102175.1"
                     /translation="MIRTSLSIVALFGSLILVSKWLHESNASAQPATSPASPAAATST
                     QPAAPIDAPRPDLPSAGSREPQRTLPTAQYGKRPVRLVSFDFDNNLPSLFNETDEENA
                     GKSEFGSGPPAYSDANDAESGDADDAITPAKPKDAPVELSPELLELRTKLRECLAHYY
                     FHPENVATRSPWGAMHAMIAYGVDSELIVSDKRVNAIGYLCYNGTCQNQRLFSLRGEK
                     LQAAIGPGVQGHPAQFLAMLAQSRVKTDYPIKVDGHDFTVADLIEHEKETCRPASELT
                     FKLIAFTHYLKSDETWKSNDGQDWDIPRLIKEELKQTIHGAACGGTHRMTGFSYAVRK
                     REKRGEPFEGQWLRAKKYVEDYHEYNFNLQNADGSFSSDWFARRADYGDHHRRLQTTG
                     HITEWLAFSLPQDQLTDPRMVKAVNFLTDLLMEHPNERWSIGPLGHGLHALAIYDERV
                     FGGAPGKRAEQLALARKDSKPGEKGVIKPVER"
     gene            complement(4421..5149)
                     /locus_tag="VFV87_00085"
     CDS             complement(4421..5149)
                     /locus_tag="VFV87_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102176.1"
                     /translation="MAKKSFAQKRLVLRALLLLALTAAGCADGPIPELRYLNPWIRQQ
                     WEKDEQEIATYHRRVADLAALRTQAATMPPVEQEQAAAELAQREHDEQSPVMRAELIR
                     TLGEFAAPAAQTAVMAAMGDESGHVRIAACNALSRHPSPEALQALTRSVSDDGDLDVR
                     IAAARALERFPGDQTPLALRPALDDNDPALQLAAMQSLQSLTGRRQYGNSVATWREYL
                     DGNDPTPPPGPTLVERWQQLWRWY"
BASE COUNT          947 a         1672 c         1784 g         1010 t
ORIGIN      
        1 cggcagcggg cggctggaac tggcgcaggc catcgtcgat tcagcgaatc cgctcaccgc
       61 acgggtgatc gtcaaccgcg tctgggcgca gcacttcggt gccgggcttg tccgtacgcc
      121 cagcgatttc ggcacccggg ccgaagcccc aagccatccc gaattgctcg attggctcgt
      181 cagccgcttc attgaggacg gctggtcgct gaagaagctg caccggagga tcatgatgtc
      241 gcaaacttat cggcaggcga gccaggagac agaggacagg ggacagagga cagaggcaga
      301 aggagcgaat accgatgtcg agaatcccaa atcccaaatc ccaaatccca aatcgaccga
      361 tccccaaaac cgcctcctgt ggcggatgaa cgagcggcga ttgtcgttcg aggagctgcg
      421 tgattccctg ctggcggcca gcggcaagtt ggactgcacg gccggcggca aggcagaaga
      481 tctgttctcg ccgcagttcg tgcgccgctc gctgtatggc acaatcgacc ggcagttttt
      541 gcccagcacg ctgcggatgt tcgatttcgc caacccggac ctgcacatcc cgcagcggag
      601 cgaaacgaca gttccgcagc aggccctgtt cttcctcaac cacccgctgg ctgtgggtta
      661 tgcccaggcg ctggccgccc gcaccacggc cgccgcgacc gatgagcagc gaatccagca
      721 gctttatcag ttcgcctacc agcgactgcc cacccccgaa cagacgcagg cggctcttga
      781 attcatacgc ctggcccagg gcgacacgac cggcgcgatc ccgccgaccg tcaacgcctg
      841 gcagtacggc ttcggcgcct atgacgagaa aatgcagcgc gccagcagct tcgagaagct
      901 gccgcacttc accggcagcg cctggcaggg ggggccggcc tggtccgacg ccaagctcgg
      961 ctgggcgcag cttacggcag ccggcggcca tcccggcaat gatctggccc acgccgcgat
     1021 tcgccgctgg accgccccgc gcgacatgcc aatccgcatc gagtcgctcc tcgttcacga
     1081 gccggccgaa ggcgaaggcg ttcgcggctt cctcatctgc agccgcggcg gcttattgca
     1141 cgaggccaag gtccatcacg gccaggcgga attcaatgcc gctaggctcg acgtgacggc
     1201 cggcgacacg ctcgacttcg tcgccgacat cggcaacaag ctgagccaca accagttcct
     1261 ctggcgcgcg accatcacgc cgctagacga ctccggcccc ccattcgact ccgtccgcga
     1321 cttcgaaaac caaggcaaga cccgcctcac tccctgggaa caactcgccc aagtgctgct
     1381 ctcggcgaac gagttgatgt tcgtcgatta gggccagcga gttggctgcc gaatagttga
     1441 gctacgtcgc tgggcggaca cctgggtcac tgggttttcg gccggggagg gagaagtcga
     1501 gttggtctgc gtccttgaat tcgggggggt gattgtagat gccgcttgtt tccttagcag
     1561 gcaggcggag gaatcgctgg gtctggttcg gcgcgtcgag ggcgtgccac tggttgccgg
     1621 tgaactcgaa cgtttcgggg gccgtgccgg ggccgatgtt tacgacctgc cggacttcac
     1681 ccgtgcggaa gacgaccagg ttcttttgga agacgccctt gcggctgggg acaaatttcg
     1741 cgtcggtgtt ctcctgcagg atgcggaggg gccagcgcgt gggtcggtag atcgtgttgt
     1801 gctggaccaa agcgccgtcg cagccgacga aggcgatggc cgacatgccg ccgatgattt
     1861 cgcagtcctg gacggtgatc tccttggctt cgaaatcgcc caactgcggg cggaaataat
     1921 cgagtccagt gctgccgcca atgttgatcg cccggccgcc ggcgttttca aagcggcaat
     1981 actggacgac gatctggttg ctcccccctt tggcttgcac gccgttggcc tggtcgccgc
     2041 cttcctgaaa ggtgcaactg gtgaccagtc cgcgctggca cccgaccatg tcgatggccg
     2101 atccgttgct cccccaccgc atgatgcggc agccatcgac gcggaagtca ttgacgccgg
     2161 agagcttgag tccgtcgcgg ttgccgctgg gtccaacgtc gcgaatcgtc agattgcgga
     2221 ggaacagatc gtggccaggc gactgcgacg agccgccgtc gtcgatgttg atgccgttgc
     2281 cgttcgcctt ggcgatgatc aggtcgcgca gctccagatg cgcaggcgac gagaggtgca
     2341 gcccgctgcc gcccccttcg atcaccggcg ggtctgccgg atcggcggcc gcaatgacga
     2401 tcggttggtc cttcctccct tggagcttgc ccggcgcgat gccgccacga taggtgccgg
     2461 gcgcgatcaa aatggtcgta cccggctggg cgttaccaaa ggctcgcacc aattcgtcgc
     2521 gcgaggcaac gcgcacctcg tcgccagccg ctagcgatgg ggccaaatgc agcgcagtga
     2581 tccaaaagct cagcagccag tatttgaagc ggttcatcgg gcatgcctcc cgaaccgcat
     2641 cgtagccgct cgcgccgcga tggcaacatg cgggctaccg ctcgacgggc ttgatgacgc
     2701 ccttttcgcc cggcttgctg tccttgcggg ccagggcgag ttgctcggct cgtttgccgg
     2761 gagcgccgcc gaagacgcgc tcatcataaa tcgccagtgc gtgcagaccg tggccgagcg
     2821 gaccaatgct ccacctttcg ttgggatgtt ccatcaatag atccgtgagg aagttgacgg
     2881 ctttgaccat ccgcggatcg gtgagctgat cctgggggag cgagaacgcg agccattcgg
     2941 tgatgtggcc ggtagtctgc agcctgcgat gatggtcgcc gtaatcggct cggcgggcaa
     3001 accaatcgct gctgaagctg ccatcggcgt tctgcagatt gaagttgtac tcgtgatagt
     3061 cctccacgta cttcttggcc cgcagccact gcccctcgaa aggttcgccc cgcttctcgc
     3121 gcttgcgaac ggcgtagctg aagccggtca tgcggtgcgt tccgccgcag gccgcgccgt
     3181 ggatcgtctg ctttagctct tccttgatca gccgcggaat gtcccaatcc tggccgtcgt
     3241 tgctcttcca ggtttcgtcg gatttgaggt aatgcgtgaa ggcaatgagt ttgaaggtca
     3301 actcgctggc cggccggcac gtttcctttt cgtgctcgat cagatcggcc acggtgaaat
     3361 catggccatc caccttgatc ggataatcgg tctttacgcg ggactgagcg agcatcgcca
     3421 ggaactgggc tgggtggccc tgcacgcccg gtccgatggc cgcctgcagc ttttcgccgc
     3481 gcaggctaaa cagccgctgg ttctggcagg tgccgttgta gcaaaggtag ccgatggcgt
     3541 tgacgcgctt gtcgctgacg atcagctctg aatcgacgcc gtaggcgatc atggcgtgca
     3601 ttgcgcccca ggggctgcgc gtggcaacat tctccgggtg gaagtaataa tgggcgaggc
     3661 actcgcggag cttcgtgcgc agttcgagca gttccgggga gagctcgacc ggcgcgtctt
     3721 tgggtttggc aggggtgatg gcgtcgtccg cgtcgcctga ttccgcatcg ttggcgtccg
     3781 aataggcggg cggaccggac ccgaattcgg atttcccggc gttctcttcg tctgtctcgt
     3841 taaataagga cggtagattg ttgtcaaaat cgaacgagac cagccgcaca gggcgtttgc
     3901 cgtattgcgc ggtaggcagc gtgcgttggg gctcgcgcga gccggcgctg ggcaaatcgg
     3961 gtcttggggc atcaatcgga gcagccggct gggtggaagt cgccgccgcg ggggaggcag
     4021 gcgaagttgc cggctgagcc gaagcattcg actcgtggag ccatttgctg acgaggatga
     4081 ggctgccaaa gagcgcaaca atcgacaggg acgttcgaat catgatgccg ccaattggtc
     4141 ggagtccgcg gctcggcaag gagctgttgc agcggaggta ggaacggagc ggccgcgcgt
     4201 tgttgccggc aggccgacaa ttcccggtgc gcgggccaca aggcgatccg cccatggagc
     4261 ctcgtggtag cagtgatatc ggtatgcagc gcggcgatct gtcgcaggag gcgacaaatt
     4321 gcggtcccta caaacacggg aaacaggacg gtactgccgg gcgtagcagg gtgcggggag
     4381 tttgcgggct ctgccaccgg taagcgaggc tagcacgccg ttagtaccac cgccagagct
     4441 gctgccagcg ctcgactagc gtcggtccgg ggggcggggt gggatcgttc ccatccagat
     4501 attcccgcca ggttgccacg ctgttgccgt actgcctgcg gccggtcagc gactgtagcg
     4561 actgcatcgc cgcaagctgc aacgcagggt cgttatcgtc cagagccggg cgcagggcga
     4621 gcggcgtctg atcgccgggg aatctctcaa gtgccctggc cgcggcgatc cggacatcga
     4681 ggtcgccgtc atccgaaacg gaccgggtga gcgcctgcag agcttccggc gacggatggc
     4741 ggctcagagc attgcacgcc gcgatgcgga cgtgaccact ctcgtccccc atggcggcca
     4801 tcacggcagt ctgagccgct ggggcggcaa attcgcccag tgtccgaatc aactcggccc
     4861 gcatgacggg actttgctcg tcgtgctcgc gctgggccag ctccgccgcg gcttgctctt
     4921 gctcgacagg cggcattgtg gcggcttggg ttcgaagcgc ggccaggtcc gcgacgcggc
     4981 ggtggtacgt cgcaatttcc tgctcgtcct tctcccactg ctggcggatc cagggattca
     5041 gatagcgcaa ttctggaatc ggcccatcgg cgcagccggc cgccgtcagg gctagcagaa
     5101 gcagcgcccg cagaacgaga cgtttctggg caaatgactt tttcgccatg cagttctcgc
     5161 gaggaagacg cgggtggggt atagcaaaac gacccgagcc ggaccagatg attcgccttg
     5221 gagaatctcg tcccggttgc cacaatccgg gcccaaggga gtaggcatgt tcaagcggtc
     5281 cgatgtttcc gctagcaggg ccgggttgcc ggccgatctg acccagccga cgcctcgcgc
     5341 tcaagcagga aagctgctgc tggcggcggg aattctgctg gcattggcag gagtggctct
     5401 ggccttcgat ctg
//
LOCUS       DASUGB010000007         6658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1529344, whole genome shotgun sequence.
ACCESSION   DASUGB010000007 DASUGB010000000
VERSION     DASUGB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529344"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFV87_00090"
     CDS             <1..214
                     /locus_tag="VFV87_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102177.1"
                     /translation="YPVVPRPKRPDESVNLLPLLNLRVERLESRLKLFEEENRKGAID
                     LTKLYGKSDQPLPLVPPPDDIVTGGE"
     gene            complement(296..1780)
                     /locus_tag="VFV87_00095"
     CDS             complement(296..1780)
                     /locus_tag="VFV87_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5102178.1"
                     /translation="MRSTMAAFVVLFAVPSLGAAERPNILFAIADDWSYPHATAHGCK
                     FVKTPAFDRVAREGVLFRNAFTATPGCSPSRASILTGQYSWRLREAGTHASSFPKDLP
                     VYPDALEKAGYFVGFCGKGWGPGNFKISGWERNPCGPDFSVKLPDPPAGIGNTDYAAS
                     FEKFLKERPKDQPFCFWYGGKEPHRAYAKGSGLAAGKRLQDAEVPPFLPDTPEVRSDL
                     LDYAMEVEYFDTQLGLMLAALEKLGEQDNTLVIVCSDNGMPFPRAKANLYDFGTHMPL
                     AIRWPAKVKSSRTVDDFASHVDVAPTILDAAGVRFPARADSGRGKSLLPTLQSDASGL
                     VDKSRDHIFLARERHSSSRPDNLGYPARAIRTADYLYIRNIKPDLWPAGDPKTADGKS
                     AYCDIDGSPSLSLLVSGQSDPKLKPFLDLAVARRPAEELFDLKSDPATIKNVAGDPAH
                     AAAKSKLRKQLDAYLTDSGDARMTDQGDIWETYPRYSPIREFPK"
     gene            complement(1839..3290)
                     /locus_tag="VFV87_00100"
     CDS             complement(1839..3290)
                     /locus_tag="VFV87_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102179.1"
                     /translation="MPETPHSALRIPHSELTRRALFERAGMGIGSIALSCLLGQDTSA
                     ASPNPESRAPSPHFSPRAKNVIFLHKVGAPSQLELFDHKPTLAQYDGQPCPKHLIEGQ
                     RFAFLRGHPSLLGPRFKFAKYGECGLELSELLPHLATVADDIAVVKSLHTEQINHGPA
                     QLMFHTGFGRFGRPSIGSWVSYGLGSENRDLPAYVVMITGKVAGAGSALWGSGFLPSM
                     HQGVEFRSQGDPVLFLSNPAGITPADRRRLLDGIQALNQEQLADVGDPEIATRISQYE
                     MAFRMQSSVPELMDLNDEPASVRELYGAKPGQASFANNCLLARRLVERGVRFVQLFHA
                     DWDTHGNIDTGLPARTKEVDQPMAALVKDLKQRGLLDETLVGWGGEFGRTPMLQGTDA
                     DDNPAAKPGRDHHKDAFCVWLAGGGVKGGTTVGQTDDLGYTIAQDPVHINDFHATLLH
                     LLGLNHERLTFKFQGRQYRLTDVAGKVVTKLLA"
     gene            complement(3292..5991)
                     /locus_tag="VFV87_00105"
     CDS             complement(3292..5991)
                     /locus_tag="VFV87_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5102180.1"
                     /translation="MLWLGMGLFAAATAEPDPEQAKLPPPAEREVDFVRDIQPIFAQR
                     CNHCHGEDEQEGYLRLDAKAVVMKGGKSGPLLEVGKSADSVLVHRLAGLGTEKRMPLE
                     DDPLSDEQIGLIRAWIDQGAKWPDGVGSAATSVKLHWAYVAPVNPELPSVRDAAWCEN
                     PVDRFVLARLEQERIAPSPVIDRERLIRRASLDLIGLPPSVDEVDAFLADESPDAYER
                     LLDRLLASPRYGERWATPWLDAARYADSNGYQRDGHRTIWPYRDYVIQALNADLPFDQ
                     FTIEQIAGDLLPEATLEQKIATGFHRCTTVNVEAGTDEEENRTNQIIDRVNVTGTVWL
                     GTTLECCQCHNHKYDPFTQRDYYRLYAFFNSTPKETYQRTKGSAALDFGGPELTLPLE
                     EQVAKERAGIAARRQQVATELEQCLEDEATGLAAWELEMSDSAKADEVQLPANIRKLL
                     EVPANKRKANQSNQLRSYFVGQHAAAKKMQAEMDELQKQLDALAPAATLVMADLDEPR
                     MTTILKRGNFLDPGEAVQLGTPRILPGLSGGEAESRLDLAKWLVDPANPLTPRVQVNR
                     AWAQFFGRGMVASEEDLGTQSEPPTHPELLDWLAVELRDRGWSLKRIHRRIAESATYR
                     QSSRFRSDLAARDPENLLLARGPRMRLSAEAIRDNALAVAGSLSAKMHGPPVYPPQPE
                     GVWRVTGLVDNTYRTSPGDDAHRRGVYTVRRRSAPYPSFSNFDAPDRSACVVKRPRSN
                     TPLQALTLQNDPVYVELAAALADKLLAETPTRRVSEGGTPTRSVSEADMPTRSVSEAD
                     IPTRSVSEADIPTRSVSEGISDEQLVYAFRTVLTRRPEAAELSHLRKLFDDAQSRYAS
                     DPKAAQQLRGKRPLPEGVDSAAWAAWFNVAHVLLNLDETITKN"
     gene            complement(6122..>6658)
                     /locus_tag="VFV87_00110"
     CDS             complement(6122..>6658)
                     /locus_tag="VFV87_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5102181.1"
                     /translation="LPHFVRLEARPTMEQRRMADTNRILIADDNEPNRELLEAYLADV
                     DCEVAHSVDGQDTLAKIKSFRPDLILLDVMMPKLSGFEVCQRLKEDAATRKIMILMVT
                     ALNEAGDIERAVKAGTDDFLSKPVNKIELLKRVENMLKLKDVRDENERLRRYIDEMEK
                     RDGEDGDEGPAGPVPKGK"
BASE COUNT         1108 a         2095 c         2190 g         1265 t
ORIGIN      
        1 atatcccgtc gttccgcggc cgaagcggcc ggacgaatcg gtcaacctgc tgccgttgct
       61 caatctgcgg gtcgagcggc tggagtcccg cctgaaactc ttcgaggaag agaaccgcaa
      121 gggagcgatc gacctgacga agctttacgg caaaagcgac cagccgctgc cgctggtgcc
      181 gccgccggac gatatcgtga cgggcgggga gtgacgacga atcgccgtaa gacgggtttc
      241 caacccgtcc ggacaggtta ctaacctgtc ctacgaggaa ggctgccagg ccgacctatt
      301 tgggaaactc gcggatcggg ctgtatcgcg ggtaggtctc ccagatgtcg ccctggtcgg
      361 tcatgcgggc gtcgcctgaa tcggtgagat aggcgtcgag ctgcttgcgg agtttggatt
      421 tcgcggcggc gtgggcgggg tcgccggcga cgttcttgat cgtggcggga tcggacttca
      481 gatcgaacag ctcttcggcg gggcgcctgg cgacggccag gtcgaggaag ggcttgagct
      541 tcgggtcgct ttggccgctc acgagcagcg acaggctggg cgagccgtcg atatcgcaat
      601 aggccgattt gccgtcggcc gttttgggat cgccggcggg ccacagatcg ggcttgatgt
      661 tgcggatgta gaggtagtcg gctgtgcgga tcgccctggc cggatagccg agattatcgg
      721 gccgcgaaga ggaatgccgc tcgcgggcga ggaagatgtg gtcgcgtgat ttatcgacga
      781 ggccggacgc gtcggactgc agcgtcggca gcaggctttt gcccctgcca gaatcggccc
      841 gtgccggaaa gcgaactccg gccgcgtcga gaattgtggg cgcgacatcg acgtggctgg
      901 cgaaatcgtc caccgtccgg ctgctcttga cctttgccgg ccagcggatc gccagcggca
      961 tgtgcgtgcc gaagtcgtac aggttggctt tggcgcgtgg gaacggcatt ccgttgtcgc
     1021 tgcagacgat gaccagcgta ttgtcctgtt cgcctagttt ctcgagcgca gcgagcatca
     1081 gcccgagctg cgtgtcgaaa tactcgactt ccatcgcata gtcgagcagg tcgctgcgga
     1141 cttctggcgt atccggcaga aacggcggca cttcggcgtc ctgaagccgc ttgcccgccg
     1201 ccaggccgct ccctttggca taggcgcggt gcggctcctt gccgccgtac cagaaacaga
     1261 atggctggtc cttggggcgc tccttaagga acttctcgaa actggcggca tagtcggtgt
     1321 tgccgatgcc ggccggcggg tctggcagtt tcacgctgaa atccggcccg cacgggttcc
     1381 gctcccagcc gctgatcttg aagttccccg gcccccagcc cttgccgcag aagccgacga
     1441 agtagccggc cttctccagc gcgtcgggat agacgggcaa gtccttggga aacgagctgg
     1501 cgtgcgtccc cgcctcgcgg agccgccacg aatactgccc tgtcaggatc gacgcccgcg
     1561 acgggctgca gccgggcgtg gccgtgaacg cattgcggaa cagcactcct tcccgcgcca
     1621 cgcggtcgaa cgccggcgtc ttcacgaact tgcagccgtg cgccgtcgca tgcggatacg
     1681 accagtcgtc ggcaatggcg aagagaatat tcggccgctc ggcggcaccg agtgaaggga
     1741 ccgcgaagag gaccacgaaa gccgccatgg tggagcgcat gttgaaaaac ctgccgcagt
     1801 tctccgggag ctgctagctc ccggctaaga gattcgattt acgccagcaa cttcgtcacg
     1861 accttgcccg ccacgtccgt cagccggtat tgccggccct ggaacttgaa cgtcaggcgc
     1921 tcgtggttga ggcccagcaa gtgcagcagc gtggcgtgga aatcgttgat atggacggga
     1981 tcttgggcaa tggtgtagcc caggtcgtcg gtctggccga cggtcgtgcc ccctttcacg
     2041 ccgccgccgg ccagccagac gcagaacgcg tccttgtggt gatcgcggcc gggcttggcg
     2101 gcgggattgt catcggcgtc cgtcccttgc agcatgggcg ttcggccgaa ttcgcccccc
     2161 cagccgacga gcgtttcgtc gagcaatcct cgctgcttga ggtccttcac cagggccgcc
     2221 atcggctgat cgacttcctt cgtgcgggcc gggaggccgg tgtcgatgtt gccgtgcgtg
     2281 tcccaatcgg cgtgaaagag ctgcacgaac cgcacgcccc gctcgaccag gcggcgggcg
     2341 agcaggcagt tgttcgcgaa tgacgcctgg ccgggcttcg ccccatagag ctcgcgcacc
     2401 gaggccggct cgtcgttgag atccatcagt tcgggcacgc tcgactgcat gcggaacgcc
     2461 atttcgtact ggctgatccg cgtggcgatc tcgggatcgc ccacgtccgc cagttgctcc
     2521 tggttgagcg cctgaatgcc gtccaagagg cggcggcggt cggccggcgt gatacccgca
     2581 ggattcgaca agaacagcac cggatcgccc tggctgcgga actccacgcc ctggtgcatg
     2641 ctcggcagaa agccgctccc ccaaagggcg ctccccgcgc cggcgacttt gccggtgatc
     2701 atcacgacat acgccggcag gtcgcggttc tcgctcccca ggccgtagct gacccacgag
     2761 ccgatgctcg gccggccaaa gcggccgaag ccggtgtgga acatgagctg agccgggccg
     2821 tgattgatct gctcggtgtg cagcgatttt acgaccgcga tgtcgtcggc caccgtcgcc
     2881 aggtgcggca gcagttccga tagctccaaa ccgcattcgc catatttggc gaacttgaac
     2941 cgcgggccca gcagcgacgg atgcccgcgg agaaaggcga atcgctggcc ttcaatgagg
     3001 tgcttggggc agggctggcc gtcgtactgg gccagcgtcg gcttgtggtc aaatagctcg
     3061 agctgcgatg gggccccgac cttgtgcagg aagatgacgt tcttggcgcg gggcgagaaa
     3121 tgggggctcg gggctcggga ctcggggttc ggggaagcag cggatgtatc ctggccgagt
     3181 aggcaggaga gcgcgatgct gccgatgccc atgccggcgc gctcgaagag cgcgcggcgg
     3241 gtgagttccg aatgcggaat gcggagtgca gaatgcggag tttcgggcat gtcagttctt
     3301 cgtgatcgtt tcgtcgagat tcagcaggac gtgggcgacg ttgaaccagg cggcccaagc
     3361 ggcgctatcg actccctccg gcagcggccg cttgccgcga agctgctgag cggctttggg
     3421 gtcgctcgca tagcgggatt gggcgtcgtc gaacaactta cgcaggtgcg acagctcggc
     3481 tgcttcgggc cggcgagtga ggacggtgcg gaaggcgtaa acgagctgct cgtccgagat
     3541 gccctcgctg acgcttcggg ttggtatgtc tgcctcgctg acgctgcggg ttggtatgtc
     3601 tgcctcgctg acgctgcggg ttggcatgtc tgcctcgctg acgctgcggg ttggtgtgcc
     3661 tccctcgctg acgcgtcggg ttggtgtttc ggcgaggagc ttatcggcga gggcggcggc
     3721 cagctcgacg tagacggggt cgttttgcag cgtgagcgct tgcagcggcg tattgctccg
     3781 cggacgcttc acaacgcaag cggaacgatc cggcgcgtcg aaattgctaa agctgggata
     3841 gggagcgctg cggcggcgaa cggtgtacac cccgcggcga tgagcatcat ccccgggcga
     3901 cgtgcgatac gtgttatcca cgaggccggt cacccgccac actccctccg gctgcggcgg
     3961 atagacgggc gggccgtgca tctttgccga cagactgccg gcgaccgcca gggcgttgtc
     4021 gcggatggcc tcggctgaca accgcatccg cggcccgcgc gcgagaagca gattctcagg
     4081 atctctggct gccagatcgc tgcggaacct ggacgactgc cggtacgtgg ccgactcggc
     4141 gatccgccgg tggatgcgct tcagcgacca gccgcgatcg cgcagctcca cagccagcca
     4201 atcgagcagt tcgggatgcg tcggcggctc actctgtgtg cccaggtcct cttcactggc
     4261 aaccattccg cggccgaaga actgcgccca agcccgattc acttgcacgc gcggcgtcag
     4321 tggattggcc ggatcgacga gccacttcgc aaggtccaag cgcgactctg cctcccctcc
     4381 gctgaggccg ggcaggattc gcggcgtgcc cagctgcacc gcctcacccg gatcgagaaa
     4441 attgccgcgt ttgagaatgg tcgtcatccg cggttcatcg agatcggcca tcacaagtgt
     4501 cgccgccggg gcgagggcat cgagctgctt ttgcaactcg tccatctcgg cctgcatctt
     4561 cttggcagcg gcatgctgtc cgacgaaata cgaccgcaat tggttggact gattggcttt
     4621 tcgtttgttg gctggaacct ccagcagctt gcgaatgttg gctgggagct gcacctcatc
     4681 cgccttggcc gagtccgaca tttccagttc ccaagcggcc aggccggtcg cttcgtcctc
     4741 cagacactgc tctagctccg tcgcgacctg ctggcggcga gcggcaatcc cggcacgctc
     4801 cttggccacc tgctcttcga gcggcagcgt caattccggc ccgccaaaat cgagagccgc
     4861 agagcctttc gtccgctgat acgtttcctt cggcgtgctg ttgaagaatg cgtacagccg
     4921 atagtagtcg cgctgcgtga acgggtcgta cttgtggttg tgacactggc agcactcgag
     4981 cgtcgtgccc agccagaccg tacctgtaac attgacgcgg tcgataatct ggttggtgcg
     5041 gttctcttct tcgtcggtac ccgcttcgac gttgaccgtc gtgcagcgat gaaatcccgt
     5101 cgcgatcttc tgctccagcg tcgcctccgg cagcaggtcg cccgcgatct gctcgatcgt
     5161 gaactgatcg aacggcaggt cagcattgag ggcctggatc acatagtcgc gatacggcca
     5221 aatcgtgcgg tggccgtcgc gctggtagcc gttgctgtcg gcatagcggg cggcgtcgag
     5281 ccagggcgtc gcccagcgct cgccataccg cggcgaggcc agcagccggt cgaggaggcg
     5341 ctcataggca tcgggcgatt cgtccgccag gaaagcatcg acctcatcca cgctcggcgg
     5401 caggccgatc aggtccagcg atgcccggcg aatcagccgc tcgcgatcga taaccgggga
     5461 gggggcgatg cgctcctgct ccaagcgagc caggacgaat cgatcgaccg ggttctcgca
     5521 ccatgcggca tcacggacac tcggcaattc cggattgacc ggtgcgacat aggcccagtg
     5581 cagcttgacg ctcgtcgccg ccgagccgac accatcaggc cacttcgccc cctggtcgat
     5641 ccacgcgcgg atcaggccga tctgctcatc cgagagcgga tcgtcctcca gaggcatccg
     5701 cttctcggtg ccgagtcccg ccaggcggtg aacgagcacg ctatcggcgc tcttgccaac
     5761 ctccagcagc ggcccggact tgcccccctt catcacgacc gccttggcat ccagccgcag
     5821 atagccctcc tgctcgtcct cgccgtggca gtggttacac cgctgggcaa agatcggctg
     5881 aatgtcgcgg acgaaatcga cttcccgctc cgcaggaggt ggcaatttgg cctgctcggg
     5941 gtccggttcg gcagtagcag cagcgaacag gcccattccg agccaaagca agcacgtcca
     6001 tcgaagccaa ttgcccatag ccggctgtct cctccgcgcg caattgcgcc gcagaattcc
     6061 gatgcccgaa agccctattt tattgaacgg gcggggcaaa tctcaatgca attggccgag
     6121 attacttgcc cttcggaaca ggcccggcag gtccctcgtc cccgtcctcg ccgtctcgct
     6181 tctccatctc gtcgatatac cgccgcagcc gttcgttttc gtcccgcaca tctttgagct
     6241 tgagcatgtt ttcgacgcgc ttgagcagct cgattttgtt caccggcttg ctgaggaagt
     6301 cgtcggtgcc ggctttgacg gcccgctcga tgtcgccggc ctcattcagg gcagtcacca
     6361 tcaaaatcat gatcttgcgg gtcgccgcgt cctctttcag ccgttgacag acttcaaagc
     6421 ccgagagctt gggcatcatc acgtcgagca gaatgaggtc gggccggaac gacttgattt
     6481 tggcgagcgt atcctggcca tcgacggagt gcgccacttc gcaatcgacg tccgcgaggt
     6541 acgcctccag gagctcgcgg ttcggctcgt tgtcgtcggc gatgagaatc cgattcgtgt
     6601 cagccattcg tcgctgctcc atcgtggggc gggcttccag cctgacaaag tggggtag
//
LOCUS       DASUGB010000008         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_834933, whole genome shotgun sequence.
ACCESSION   DASUGB010000008 DASUGB010000000
VERSION     DASUGB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834933"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFV87_00115"
     CDS             <1..354
                     /locus_tag="VFV87_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5102182.1"
                     /translation="ASTQEVRVHYATANGSAKTSNKDYLAASGTIRFAPGQTTHTITI
                     TINGDTKKENNESFKVKLSSAVNGEIEDGQGLGTILNDDAGRTSKARHRASWAWAVDA
                     AMEDVYRGRLKKHRR"
     gene            437..1531
                     /locus_tag="VFV87_00120"
     CDS             437..1531
                     /locus_tag="VFV87_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX5102183.1"
                     /translation="MSALVDAPVKQQAEPRTAPPAPVPFRYAQTDSFVELLQQLGATL
                     LVTTYQANKLLAVRACENGLSTLVRTFDRPMGLAVDGSRLALGTRKEVWFFRNASDIA
                     PKIDPLGKHDACFLPRSSHVTGDVGIHEIAYAGGELWLVSTRFSCLATLHPDYSFVPR
                     WRPPFITALAAEDRCHLNGLAMVEGRPTFVTALGTTDQRDGWRANKPQGGCIIDVTSG
                     EFVARGLSMPHSPRWHNGQLWVLESGTGTLLRVDLITGKAETIARMPGFARGLALCGP
                     WALVGLSKIRPTSAMDGVPLASRRNELKCGISVVDLRNGQTFALLEFQSAVEEIFDIQ
                     LLPGLHFPEIVGFRNDSLDHTFIVPPADAC"
     gene            1728..1952
                     /locus_tag="VFV87_00125"
     CDS             1728..1952
                     /locus_tag="VFV87_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102184.1"
                     /translation="MEAAGIEHCPKSSANSGDGGQSGAECGALGTQNAPLDPDLAAVV
                     DAWPKLPEAIKAGILAMVTAARRGGAEGDR"
BASE COUNT          385 a          650 c          640 g          342 t
ORIGIN      
        1 gccagcaccc aggaagtgcg cgtgcactac gccacggcca acggcagtgc caagacaagt
       61 aacaaggact acctggcggc ctcgggaacc attcgcttcg cacctggcca gaccacccat
      121 acgatcacca tcaccatcaa cggggatacg aagaaggaga acaacgaatc cttcaaggtg
      181 aagttgtcca gtgccgtgaa cggcgagatc gaggacggcc agggattggg aacgatcctc
      241 aacgacgacg ccggccggac cagcaaggcc aggcatcgtg cttcctgggc ctgggccgtc
      301 gatgcggcaa tggaggatgt ttaccgcggc cggctgaaga agcatcgccg gtaggtcagt
      361 tgggtactgt tgcacgcgag acgccgcccg gtcgggggcg gcttttcgtg cgtgcgttcc
      421 gcatgtggaa agacgcatgt ccgcactcgt tgacgcgccg gtcaaacagc aggccgaacc
      481 gcgaacggcg ccacccgcgc cggtcccgtt tcgctacgct cagaccgata gcttcgtcga
      541 actcttgcaa cagctcggcg cgaccttgct cgtgaccacc taccaggcca acaagctgct
      601 cgcggtgcgg gcttgcgaga atggactctc cacgctggtg cgcacctttg accggccgat
      661 gggcttggcc gtggacggat cccggctggc cctcggcact cgaaaggagg tctggttctt
      721 tcgcaacgca agcgacatcg cgccaaaaat cgacccgctt ggaaagcacg acgcttgttt
      781 tctgccgcgc tcctcgcacg tcaccggcga cgtcggcatt catgagatcg cctatgccgg
      841 cggcgagctg tggctggtga gcacgcgttt ttcctgtctg gcgacacttc atcccgacta
      901 cagcttcgtt ccccgctggc gacccccgtt catcaccgcg ctggctgccg aggatcgctg
      961 ccatctcaac ggcctggcaa tggttgaagg ccgtcccaca tttgtcaccg ccctgggcac
     1021 gaccgaccag cgcgatggct ggcgcgccaa caagccgcag ggcggctgca tcatcgatgt
     1081 gaccagcggc gaattcgtgg cccgcggcct gtcgatgccc cactccccgc gctggcacaa
     1141 cggccagctc tgggtgctcg agtcagggac aggcactttg ctgcgcgtgg acctgatcac
     1201 cggcaaagcc gagacgatcg cacgaatgcc gggcttcgcg cgcgggctgg cgctgtgcgg
     1261 cccctgggcg ctggtcggac tttccaagat caggccgact tcggcaatgg atggcgtgcc
     1321 cctggcgtcg cgccgcaacg agctgaaatg cggcatatcg gttgtcgatc tgcgcaatgg
     1381 ccaaaccttc gccctgctgg agtttcaatc ggcggtggaa gaaatctttg acatccaact
     1441 cttgccgggc ctgcacttcc cggagatcgt gggctttcgc aacgattcgc tcgatcacac
     1501 atttattgtg ccgcctgcgg atgcttgttg atcgggcgcg gccgaaaatc gatgggcacg
     1561 cgccacgcga gactgtaact ttccgctctg acattggcca tcgccccaac agtcgtccct
     1621 cacgagcgaa tccaagcggc ggacaagaag gacaaaaacc cctgcggtga caggggttat
     1681 ggcacgactc ctcgcgccgc gtcggctact gtcgcaatcg gccaaaaatg gaggcggcgg
     1741 gaattgaaca ttgcccgaaa tcgtcggcaa atagcgggga tggcggccaa agcggcgcag
     1801 aatgcggcgc actcggcaca caaaacgccc cgctggaccc cgatctggcg gcggttgtcg
     1861 atgcttggcc caagctgccc gaagccatca aggccggcat cctggcgatg gtgacggcgg
     1921 cacggcgggg cggcgcggag ggcgaccgat gaccgcaacg ggtggctgcc gacgctgcgg
     1981 gatcgacgct gacggccttc cggggcgaca gggcaac
//
LOCUS       DASUGB010000009         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_556987, whole genome shotgun sequence.
ACCESSION   DASUGB010000009 DASUGB010000000
VERSION     DASUGB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556987"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VFV87_00130"
     CDS             <1..1459
                     /locus_tag="VFV87_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX5102185.1"
                     /translation="AVENGRWVIASHQNYPADAENQMRDAAAGLIDIKGLAVASDVGG
                     DHELYGVVEPDQSKLKVGDKGVGTLVAMQDEKGNDLMRLVVGKPVEGAAGHHFVRKPA
                     EERVWVAELNLDKLSTEFDKWIEKDLLKLSPFDVSKVTLKDYSLLPTQTGTALLQRME
                     ASLTYDSAATSPWSLDKMIVYSRSGEPKDAPLTEQEELNKQKLDDLRNGLDDLKIIDV
                     ARKPESLGKLLKEGLEDFSETERESLASFGFVPARGPDGTLDLFASNGELIVDMKDGV
                     RYVLRFGKVASAEQASSTAGSDSGAKDDVGADAIRVSRYLFVSAQLSPLTLTQPMLEP
                     EPAGPENPAPETPAGKPAEKPADDKAAAEGDDPDAPKTEPAADPAKAERERIKRENDR
                     KMTEYREKKIKAENRVKELNARFADWYYVVSEDIYKKLRLSRADFVKEAATAAEEGFG
                     VDAFRNLEDGGVKGVPAPAAGSLPTTPSPAFPSLP"
     gene            complement(1531..>2569)
                     /gene="pilM"
                     /locus_tag="VFV87_00135"
     CDS             complement(1531..>2569)
                     /gene="pilM"
                     /locus_tag="VFV87_00135"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX5102186.1"
                     /translation="LQGLGLAQIEADLTPQEKGGMLGMLPGISFGRKAGNMAWGLDLS
                     DFALKAIKLSRRGKTEEVQIEACEYILHSTPLTHPEVDQEKPQIIEETLQDFVGRAGE
                     LKGVKVCIGFPGQRVLGRFFELPPMPAKKVNGSIQYEAVHQLPVSLEELCWSHQILDP
                     SNGRIADDQPRRVLIQAARDAHVRDRVATFKLAGITADFVQSDCIALHNALVHELFAD
                     TDPSLNAEAITAIDVGTESTNIVVSSPACVWFRTVTQGGESFTRELVKQLKLTHEQAE
                     QLQRNPAKARRFSQLQAAIEPLFVQLASEIDRSLTTYAKLYPDHPIQQAYGLGGGFQM
                     HGLLRFLRSGK"
BASE COUNT          508 a          789 c          833 g          439 t
ORIGIN      
        1 cgccgtggag aatggtcgct gggtgatcgc ctcgcaccag aactatcccg ccgatgccga
       61 aaaccagatg cgcgacgcgg ctgccgggct catcgacatc aaggggctgg cggtggccag
      121 cgacgtcggc ggcgaccacg agctgtatgg cgttgtcgaa cccgatcaat cgaagctcaa
      181 ggtcggcgac aagggagtcg gcacgcttgt ggcgatgcag gacgaaaagg gcaacgacct
      241 gatgcggctg gtggtgggca agccggtgga aggcgccgcc gggcaccact tcgtgcgcaa
      301 gcccgccgag gagcgcgtct gggtagccga gctcaacctg gataagcttt cgaccgaatt
      361 cgacaagtgg atcgagaagg acctgctcaa gctcagcccc ttcgacgtct cgaaggtcac
      421 gctcaaggat tattcgctgt tgccgacgca aactggaacg gccctgctcc agcggatgga
      481 ggccagcctg acctacgaca gcgccgcgac cagcccctgg tcgctcgaca agatgatcgt
      541 ctattcgcgc agcggcgagc cgaaggacgc tccgctcacc gagcaggaag agttaaacaa
      601 gcaaaagctg gacgatcttc gaaacggtct ggacgacctg aagatcatcg acgtggcgcg
      661 caagccggaa tcgctcggca agctgctgaa ggagggcctc gaagatttca gtgagaccga
      721 gcgagagtcg ctcgcctcgt ttggattcgt tcccgctcga gggccggacg gcacgctgga
      781 tttgttcgct tccaatggcg agctgatcgt cgatatgaag gatggtgtgc gctacgtgct
      841 gcgattcgga aaagtggcca gcgccgagca ggcctcgtca actgccggca gcgattccgg
      901 tgcaaaagac gacgtagggg cggacgcgat cagagtaagt cgctacctgt tcgtctcggc
      961 acagctttcg ccgctgacgt tgacgcagcc gatgctcgag ccggagcccg ctgggccgga
     1021 gaatccggct cccgaaactc ctgcggggaa gcccgcggaa aagccggccg acgataaggc
     1081 cgcggcagaa ggggacgatc ccgacgcgcc gaagaccgag ccggctgccg acccggccaa
     1141 ggccgagcgc gagcggatca agcgcgagaa cgaccggaag atgaccgagt atcgcgagaa
     1201 gaagattaaa gccgagaatc gcgtgaagga gctgaacgcc cgctttgccg attggtatta
     1261 cgtggtgtcg gaagacatct acaagaagct ccgcctcagc cgggccgact tcgtcaaaga
     1321 agccgcgacc gccgcggaag agggcttcgg cgtcgatgcc ttccgcaacc tggaagacgg
     1381 cggtgtgaaa ggcgtccccg ctccggctgc cggcagcctg ccaacaacgc cgtcgccagc
     1441 gttcccgtcg ctgccgtagg caaatgcgta ggacgaagcc gcttcgtccc aatcccgttt
     1501 cgcggacgaa gcacggcttc gtcctacggc tcatttgccg ctgcgcagga accgcaagag
     1561 gccgtgcatc tggaagcctc cgcccaatcc atacgcctgc tggatcggat ggtcggggta
     1621 gagcttggcg taagtcgtca gcgagcggtc aatttcactg gcaagttgga cgaacagggg
     1681 ctcgatcgcc gcctgaagct ggctgaagcg gcgagccttg gcgggattcc gctgaagctg
     1741 ctcggcctgc tcgtgcgtga gcttgagctg cttgacgagc tcgcgagtaa agctctcgcc
     1801 cccctgtgtc acggtgcgga accacacgca agccggcgag ctgacgacga tgttcgtgct
     1861 ctccgtccct acgtcgatgg ccgtgatcgc ctcggcgttt aacgacggat cggtgtccgc
     1921 aaacagctcg tggaccagag cgttgtgcag ggcgatgcag tcgctctgca cgaaatcggc
     1981 cgtgatgccg gccagcttga acgtggccac gcggtcgcgg acgtgcgcgt cgcgggccgc
     2041 ctggatcagc acgcgccgcg gctggtcgtc agcgattcga ccgttgctgg gatctaggat
     2101 ctggtgcgac cagcacaact cttccagcga aaccggcaac tggtggacgg cctcgtactg
     2161 gatcgaacca ttgactttct tggccggcat cggaggcaat tcgaagaagc gccccagtac
     2221 ccgctgacca ggaaatccaa tgcagacctt gacgcccttc aattcgcccg cgcgccccac
     2281 gaagtcctgc agcgtctcct caatgatttg tggcttctcc tggtccactt ccgggtgcgt
     2341 gagcggcgtg gaatgcagga tgtattcgca ggcctcgatc tgtacctctt ccgtcttgcc
     2401 acggcgcgag agcttgatgg ccttgagggc gaaatcgctc aggtcgaggc cccaggccat
     2461 gttgcctgcc ttgcggccaa acgaaatgcc ggggagcatg cccagcattc cacctttctc
     2521 ctgcggcgtc aggtccgcct cgatctgcgc cagacccagg ccctggagt
//
LOCUS       DASUGB010000010         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1390743, whole genome shotgun sequence.
ACCESSION   DASUGB010000010 DASUGB010000000
VERSION     DASUGB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390743"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VFV87_00140"
     CDS             complement(<1..2138)
                     /locus_tag="VFV87_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX5102187.1"
                     /translation="MPKLALAICRHRASLTCSAAVLLMLPVAGALAADRYLAWLEDGS
                     RLTARAMPTWPMPGAAYRLENQDLLASSNSVRLVRDRQAAVRLAGPYVVLANGDIITG
                     ALQQLEADDGRLGIVPRVKVQLESPLMPVTGTGIAIRTDRVLRIVGSSAAGSAPPLPG
                     AVVLADGRRLIARAIRWKQNGLSILTEGGIVEANFGDLVDVSFPTVDHISAVLDDNLW
                     AGSGQGAAIVRLQTQGGAVLTASRISREVERIRRRGRSTSDAYYYVQPAWADQPLAIP
                     EPLVAWCGYRRADEAPLSLLPSETLVDRRLIGRPQPWLANRAADGGLLASDDRESDLG
                     LVTHSHSEIAFDLPLAARTLEMSVGLDRTTDGGGCVRCKVLAEKSGGEVLWDSGIIQS
                     RDGLQETGPLDVSGLVRVVLVTEFAHEGRPAGADPFDIRDQVCWLAPLVKLDLSGSGR
                     AERLQAILSGASEWDLVGDGWQDAEIADRWSVGTSSWEAILTLPRDLELHLVRQLRVS
                     RGADIVEFLTACPREMSAHDFLLTANGKPLIWQNNADPADLYKWVSRYGTQRWRDDDD
                     DFVLTDRLAYWWDLSEFRGQDVTLELTLRGRRAWMEIAWRGLSIRSAIAGSQDQEPLA
                     PDVPLTSLTPVRSDSHKDRGFPRQDALPMGRFGEPIRFLGQKFIGGYGMKRDSAVAFE
                     IEPEYRRFVALAGCCSYVVGPLQVVIDDRVV"
     gene            2704..4218
                     /locus_tag="VFV87_00145"
     CDS             2704..4218
                     /locus_tag="VFV87_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102188.1"
                     /translation="MRTLHANVVSALVLGTLTFAAAANAHAQACGCPSYRVVQKVVYD
                     QVPTTAYRLQYETVFEEQQVTTLRPEWITETRERRFTVAKPVTETSTREERYIVRRPV
                     YETEYREESYDKVSYVTETEMRQERYQVSRPVYETQVREQQYTVRRPVQETVVQQQAY
                     TAYQPVTSYQTQYVDQGQYVTTLMPTAPVVRNRLWWVQRGYRVDPATGAAVWQRGGLH
                     WVPTAGPSVSQPVTTYMPNVVAQQIPVTSYQPVTMTQDVPVNVTRYVDEVVTQQIPTQ
                     VMRMETTEEVRDVPVTVQRPVTERVTYQVPVQKVRYEEEEHVRQIPVTVQRIEYEEQV
                     EQTPVQILRYVNETKAVQVPRTVGRWVAYETTRLVPRVVTMRVPLDGWYDGVTYEGPV
                     TSYYSTPIPPAPPAASSRKVIVQKPADKTQSQKPAAKPAVKATEEPAEGGLTPSEGRG
                     DEPTPARPKPAAGEAKDADPTGQPALGDKEPPAAGLELNSPAGASRRGNSDRHA"
     gene            4382..4924
                     /gene="ilvN"
                     /locus_tag="VFV87_00150"
     CDS             4382..4924
                     /gene="ilvN"
                     /locus_tag="VFV87_00150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5102189.1"
                     /translation="MRHVLSAIVQNVPGVLAHISGMLASRGYNIDSLAVGETEDPNLS
                     RMTFVVVGDDRVLDQVRKQLEKIVTVVQVEDVSSRDHVERDLMLLKVEAPPGPKRSEI
                     RELVEIFRAKIVDVGAGEMMIEIAGRESKIEAFIERMRPYGILELVRTGRIAMVRGKN
                     RQPKTEEEAEEMNEVGVSGE"
BASE COUNT          970 a         1571 c         1571 g          827 t
ORIGIN      
        1 accacgcggt cgtcgatcac gacctgcagc gggccgacaa cgtacgagca gcatcccgcc
       61 agggccacaa accggcgata ttccggttcg atctcaaagg cgaccgcgct gtcgcgcttc
      121 atgccatagc cgccgatgaa cttctggcct agaaagcgaa tcggctctcc gaagcgcccc
      181 attggcaacg cgtcctgccg ggggaaaccg cggtccttgt gcgaatcgga ccgcacgggc
      241 gtcagcgaag tgagcggcac gtccggcgcg agtggctcct ggtcttggga gccggcgatg
      301 gccgaccgga tcgacaggcc gcgccaggcg atctccatcc atgcgcggcg gccacgcaac
      361 gtaagctcca gcgtcacgtc ctggccgcgg aattccgaca aatcccacca gtacgccaac
      421 cggtcggtga ggacaaaatc gtcatcatca tcgcgccagc gctgggtccc gtagcgcgag
      481 acccacttat agagatcggc cggatcggcg ttgttctgcc agatcagcgg tttcccgttg
      541 gcggtgagca ggaaatcgtg cgcggacatt tcgcgcgggc acgcagtcag gaattccacg
      601 atatcggccc cgcgcgacac gcgcaactgc cgcacgaggt gcaattctaa gtctcgtggg
      661 agcgtcagga tcgcctccca cgacgaggtt cccacgctcc accggtcggc gatttccgcg
      721 tcctgccaac catcacccac caggtcccac tcgctcgcgc cggacaaaat cgcctgcaga
      781 cgctcggccc ggccgctgcc ggagagatcg agttttacga gcggcgccag ccaacaaacc
      841 tggtcgcgga tgtcgaacgg atcggcgccg gccgggcggc cctcgtgcgc gaattccgtc
      901 accaggacga cccgcaccag ccccgaaacg tcgagcgggc cggtttcttg caggccatcg
      961 cgggactgaa tgatcccgct gtcccagagg acttcgcccc cggatttctc cgcgagaacc
     1021 ttgcaccgca cgcagccgcc gccgtcagtt gtgcgatcga ggccgacgct catttcgagc
     1081 gtgcgggcag ccaaggggag gtcgaaggca atctcactat gcgaatgggt gaccaggccc
     1141 agatccgatt cgcggtcgtc ggaggcgagc agcccgccgt cggccgcgcg gttggccaac
     1201 caaggctgcg gccggccgat caggcggcga tcgaccagcg tctcgctggg aaggagcgac
     1261 agcggggcct cgtctgcccg ccggtagccg caccaggcca cgagcggctc ggggatggcg
     1321 agcggctgat cggcccaggc gggttgcacg tagtagtatg cgtcgctggt cgagcggcca
     1381 cggcggcgga tgcgctccac ttcgcggctg atgcgggaag cggtcagcac cgctccgccc
     1441 tgcgtttgca gtcggacaat cgccgcccct tgcccgctgc cggcccagag gttgtcgtcg
     1501 agcacggcgg aaatgtgatc gaccgtcgga aagcttacat ccacgaggtc gccgaaattt
     1561 gcttcgacaa tccccccttc ggtcaggatc gacagcccgt tttgcttcca gcgaatcgcc
     1621 cgagcgatga gccggcgccc atcggccagc accaccgcac ccggcaacgg cggagcgcta
     1681 cctgccgcgc tggagcccac gattcgcagc acgcggtcgg tgcggatggc aattcccgtt
     1741 ccggtcaccg gcatcagcgg cgattcaagc tgcaccttta cgcgagggac aattcccagg
     1801 cggccgtcat cggcctcgag ctgctgcagg gcgcccgtga tgatgtcgcc attggcgagc
     1861 acgacgtagg gcccggccag ccgcactgcg gcctggcgat cgcgcaccag ccgcacggaa
     1921 ttcgacgacg ccagcagatc ctggttttcc aggcggtagg cggcgcccgg catcggccag
     1981 gtcggcatcg cccgggcggt caagcgtgag ccgtcctcaa gccaggcgag ataccgatcg
     2041 gcggccagcg cgcccgcgac gggcagcatc aaaagcaccg cggctgagca ggtgagagaa
     2101 gctcgatggc ggcagatcgc aagggcgagt ttgggcatac ggcaagcagc tccggcctga
     2161 atctacctaa acccaattgc tcattgcggg tttcgcgtat tctcgaatct tgagcgagcg
     2221 cattccatcg gtcaacttcg cagcgagagc cccaccctgc gattgcgtca ctttattaag
     2281 taattcatga tgacattctg aatactttcc atcgacgcaa ttcgcagtgg cgcgcggcga
     2341 tgtccacatc gcgcctggca tcgcaggtcc gtttattcca attaacgagg ttgggaagac
     2401 ttgcgtccta actcgatgtg gcgaaaaaac atcgggaaaa tgcagtccaa acagtgtcgg
     2461 cagcaagctc cgcagacttc agattttttt ttgcgacgta actgcatccg cctcaagcgg
     2521 ttagtcgctt gatagccctg atagcacaaa aaaatccgtt tgcatcgaca gcggttttcc
     2581 gccacatttc taggtagtag cgcggcgcga gtgattgcgc ggttgtttgc tgttggcgct
     2641 tcgagcagga cgcttcgacc agccagagca ccctgagaaa gcacccctgc ggtgagaagc
     2701 gttatgcgaa ccctccatgc caatgtcgtt tccgccctcg tcctgggcac gctcactttt
     2761 gccgcggctg ccaacgcgca cgcgcaagct tgcggctgcc cgagctatcg cgtcgtgcag
     2821 aaggtcgtct atgaccaggt gccgacaacg gcttatcgac tgcagtacga aacggtattc
     2881 gaagaacagc aggtgacgac gcttcggccg gaatggatca ccgagacacg ggagcggcgg
     2941 ttcacggttg caaaaccagt gaccgagact tcgacgcgcg aagaacgcta tatcgtccgg
     3001 cggcccgtct atgagaccga atatcgagaa gaaagttacg acaaggtctc gtatgtgacc
     3061 gagaccgaga tgcggcagga gcggtatcag gtcagccgcc cggtgtatga aacgcaagtt
     3121 cgcgaacagc aatacaccgt ccgccgcccc gttcaggaga cggtcgtcca gcagcaggct
     3181 tacacggcct accagccggt caccagctac cagacgcagt acgtcgatca aggtcagtac
     3241 gtgacgacgt tgatgccgac cgctcccgtc gtccgcaacc gcctgtggtg ggtgcagcgc
     3301 ggctatcgcg tcgatccggc gaccggcgcc gccgtctggc agcggggcgg tttgcactgg
     3361 gttccgacgg cgggcccatc ggtctctcag ccggtgacga cttacatgcc gaacgtcgtc
     3421 gcccagcaga ttcccgtgac gagctatcaa ccggtgacga tgacgcaaga cgttcccgtc
     3481 aacgtcacaa ggtacgtgga tgaagtggtg acgcagcaga ttccgacgca ggtgatgcgg
     3541 atggagacca cggaggaggt gcgcgacgtg ccggtgacgg tacagcggcc cgtgaccgaa
     3601 cgggtgacct accaggtacc cgtgcagaag gtccgctatg aggaagaaga gcacgtccgc
     3661 cagattccag ttaccgtgca gcggatcgag tacgaagagc aagtcgagca aacgccggtg
     3721 cagatcctgc gctacgtgaa cgagactaag gccgtgcagg ttccgcggac ggtgggtcgt
     3781 tgggtcgcct atgaaacgac gcggctggtt ccgcgcgtcg ttacgatgcg cgtcccgctg
     3841 gacggctggt acgacggagt gacctacgaa gggccggtca ccagctatta ctccacgccc
     3901 attccgccgg ctcctcctgc cgcgtccagc cgcaaggtga ttgtgcagaa gccggccgat
     3961 aagacgcaga gccagaagcc cgccgcgaaa ccggctgtga aggcgaccga agagcccgcc
     4021 gagggcggcc tcacgccgtc cgaaggcaga ggcgacgagc cgacgcctgc caggccgaag
     4081 cccgccgccg gcgaggctaa ggacgccgat ccgaccggcc agccggcatt gggtgacaag
     4141 gaaccgccag cggctgggtt ggaactgaac tcgccggccg gggcgtcccg ccggggtaat
     4201 tcggaccggc acgcataagt cgagtgattc tcaccgcgag cagcgcgatc aggccgggcc
     4261 attcacctgg ccttttcttt tgacgaacct cgcgacgcga gttcgcgtcc gtcgcgccgt
     4321 ccttgtcgcc cttcggcctc ctcactagga tagcggtttc cgctttggga agtgttttcc
     4381 gatgcgacac gtcctatccg cgattgtcca gaacgttcct ggtgtgctgg cgcacatttc
     4441 gggcatgctg gcctcgcgcg ggtacaacat cgattcgctc gccgtcggcg agacggaaga
     4501 cccgaacctg tcgcgaatga cgttcgtcgt cgttggcgac gaccgggtgc tcgaccaggt
     4561 ccgcaagcag ctcgagaaga ttgtgaccgt ggtccaggtc gaggacgtca gctcgcgcga
     4621 ccatgtcgag cgggacttga tgctcctgaa agtcgaggcg ccacccggac caaagcggtc
     4681 cgaaatccga gaactggtcg agattttccg ggccaagatc gtcgatgtcg gcgcgggcga
     4741 aatgatgatc gaaatcgccg gccgcgagag caaaatcgag gccttcattg agcggatgcg
     4801 cccctacggc atcctggagc tcgttcgcac cggccgcatc gccatggtcc gcggcaaaaa
     4861 ccgccagcca aagaccgagg aggaagcgga ggagatgaat gaggtcggcg tgagcggtga
     4921 gtaatcagtt gtaagcgtc
//
LOCUS       DASUGB010000011         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_418405, whole genome shotgun sequence.
ACCESSION   DASUGB010000011 DASUGB010000000
VERSION     DASUGB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418405"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1325
                     /locus_tag="VFV87_00155"
     CDS             291..1325
                     /locus_tag="VFV87_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEX5102190.1"
                     /translation="MKEPRNRVIQLRRFGDADGLEVMDAPMPTAGQGEVRVRVLASGL
                     EYTDVVIRRHLYPQTMRQRPPFVLGYDVVGEIDQLGDGVSDHHIGERVADMTVLGSNA
                     AFRTLRASDLTSVPADVDAAEAAALILSWTTAYQLLHRAAQVRRGQRVLVQGAAGAVG
                     QALLVLGKLAGLELWGTVRGEHSTLVRELGATPIDYQREDFTRTLPGGFDVIFDGIGE
                     DGYRRSFAALKRGGLLCAYGYTASVQAQRRLLTILTWIARVYVWRRLLSWLPGGKHIR
                     VYSINVMRARHPDWFREDLERLLGLLVTHAIQPRVAERISFEQVPQAHRRLEAGGLEG
                     KLVLCPEFSS"
     gene            complement(1454..2431)
                     /locus_tag="VFV87_00160"
     CDS             complement(1454..2431)
                     /locus_tag="VFV87_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5102191.1"
                     /translation="MLRRICAVALAALGLFAFCIPGSAQQYPSRTVTIIVPYPAGGPT
                     DETARIVANFLSKKLGQNFIVENVTGGSTIIATGRVANAAPDGYTLLLHNLQITANVT
                     LIKNLPFDTEKAITGVMLVNKNPLVLVGRPGLPANNLKDLIALMKTQRLREALPGFGT
                     TGHLTSALFTQEAKVQLDFIPYRGAAPAMNDLLGDHVDLFVGTPQSVVPHVAAGKLKA
                     YGITAKEKSPQLPTAESLVDALGPKFDIVYWQGMFAPAGTPAAVINTLNAALRDAVSD
                     PDILKTWTKEGFAPFPKEQRSVAAANAMMKSEIARWGQVIRDNNIQVTQ"
     gene            complement(2472..3956)
                     /locus_tag="VFV87_00165"
     CDS             complement(2472..3956)
                     /locus_tag="VFV87_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5102192.1"
                     /translation="MVDPEKWVPDGYACLRIDSRGAGHSPGYLDVWCERETQDLYQCV
                     EWAGTQPWSSGKVGINGISYYAMNQWTVGALRPPHLAALCIWEGSSDYYRELCRHGGI
                     LSDFLNSWHPRQVASVQYGVGTRGKKSVVTGEYVAGPETLPKEELAKNVADTPGEPKR
                     RRLYDNYYSKRIAKFEDIEAPLLSAGNWGGMGLHTRGNFEGWLRAGSRQKWLEVHGDT
                     HFTHFYSRYGEALQKKFFGHFLKGENTGWDKQPRVSLNVRHPGEKFVLRAENEWPLAR
                     TQWTKYFLQPDGKTLSPDTPSQATTLGYETKGDGLTFSTPPMTKDLEATGPVAAKLWV
                     SSETTDADLFLALRVFDPAGKEVTFIGSNDPRTPVGLGWLRASHRKLDPKLSKPYRPW
                     HTHDEERPLKPGEPVELDVEVWPTSIVVPQGYRLALTIRGKDYEVDGTDIALPNAPYP
                     MRGVGPFLHVDKDDRPRAIFDCRNTLHFAPGRQPYLLLPIIPAA"
BASE COUNT          709 a         1220 c         1287 g          844 t
ORIGIN      
        1 actcctccct ggcggctggg tcgtctgcgc ctgccgcaag ctgtcgataa tgttcggctt
       61 gctcgcggta atagtcggag ttgtgcatgc gagaccggcg ttcagtgtcg gtacgactaa
      121 acactaacgc cgaaggtccc gcattgtgtc aggaagtctg acctaatgga ttcaaagacg
      181 ctatgtcatg gctatggggc cagaagcgga tatttgatcc tcgtcaagga caaaccgatt
      241 ttcctctcga ttgtataagt ggcaccaatc gcgaatggtg aggagtgccc gtgaaggagc
      301 cacgtaaccg ggttattcag ctcaggcgct tcggcgatgc cgacggcctt gaggtgatgg
      361 atgctccgat gccaacagct ggccagggcg aggtgcgggt tcgagtactg gcctcaggtc
      421 tcgagtacac agacgtagtg atccggcgcc acctttaccc gcaaacaatg cggcaacgac
      481 cgccattcgt gctgggctat gatgttgtag gtgaaatcga tcagctcgga gatggtgtga
      541 gcgaccatca tatcggcgag cgtgtcgccg atatgaccgt tctgggctcg aacgccgcct
      601 ttcgcacgct acgtgccagc gacctcacca gcgtgccagc tgacgtcgat gcggctgagg
      661 cggccgcgtt gatcctgagc tggacgaccg cgtatcagct tcttcaccgg gcagcccagg
      721 tgcggcgagg tcagcgcgtg cttgtgcagg gagctgctgg cgccgtcggt caggccctac
      781 tcgtactcgg caagctggct ggccttgagc tttggggtac ggtgcgcggt gagcattcaa
      841 ctttggtccg cgaactcggc gccacaccca tcgactacca gcgcgaagac ttcacgcgca
      901 ccttgccggg aggttttgac gttattttcg acggaatcgg cgaggacggt tatcgacgct
      961 cattcgcggc gctcaagcgc ggcggactgc tgtgcgccta cggctacacg gcgagtgtgc
     1021 aggcgcaacg tcgtctgctc accatcctta cgtggattgc gcgcgtatat gtttggagac
     1081 ggttgctgag ttggttgcct ggcggaaagc acatccgcgt ctactcaata aacgtcatgc
     1141 gggcgcgaca tcccgactgg tttcgggagg acctggagcg gctccttggc ttgttggtaa
     1201 ctcacgccat ccagccgcgc gttgccgagc gaatctcatt tgaacaggtc ccgcaggccc
     1261 accgccgtct cgaggccggt ggtctcgagg gcaagctcgt cctttgccct gagttttcgt
     1321 cgtaacccgg cgggccaaat tgccgatgtc cgctttgggt cataagcgga cgttttgcag
     1381 cgcaaaccga atgcccgctt tgggcaaaaa agcggacatt gcgcgccccg gaatgacgag
     1441 gggagagccg atactactgc gtgacctgga tgttgttgtc gcggataacc tggccccagc
     1501 gggcgatctc gctcttcatc atggcgtttg ctgccgccac cgagcgctgc tccttcggaa
     1561 atggcgcgaa gccttctttc gtccaggtct tgaggatgtc aggatcggat actgcgtccc
     1621 gcaaggcggc attcagcgta ttgatcaccg ccgcaggcgt tcccgccggc gcgaacatgc
     1681 cctgccagta gacgatgtcg aacttcggcc ccagcgcatc gaccaggctt tcggcagtcg
     1741 ggagctgcgg ggacttttct ttcgccgtga tgccgtaagc cttcaacttt ccggccgcaa
     1801 catgcgggac gaccgattgt ggcgtgccca cgaacagatc gacatggtcg cccaggagat
     1861 cattcatcgc gggcgcggcg ccgcgatacg ggatgaaatc gagttgcacc ttggcctcct
     1921 gcgtgaacag cgcggatgtg aggtgtcccg tggtcccgaa gcccggcagg gcctcgcgca
     1981 ggcgctgcgt cttcatcagg gcgatcaagt ccttaaggtt gttcgccggc aggccaggtc
     2041 tcccgaccag taccagcgga ttcttgttga ccaacatcac cccggtgatg gccttctccg
     2101 tatcgaaggg caggtttttg atcagcgtga cgttggccgt gatctgcagg ttatgcagga
     2161 gcagcgtgta gccatccggc gcagcgttcg cgacccggcc ggtggcgatg atcgtgctgc
     2221 cgcccgttac gttctcgacg atgaaattct ggccgagctt cttgctcagg aaattcgcga
     2281 cgatgcgcgc cgtttcgtcg gtgggaccac ccgcagggta ggggacgatg atggtgacgg
     2341 tgcggctcgg atactgctgc gctgatcccg gaatgcagaa agcaaacagg cctagcgcgg
     2401 ccaaagcaac ggcacagata cgcctcagca tgacctcatc ctcccattta cgtttatgat
     2461 tttcagcaag cttacgccgc gggaatgatc ggcaacaaga ggtagggctg gcgtccgggg
     2521 gcgaaatgca gcgtattccg gcagtcgaat attgcccgcg gccgatcgtc cttgtcgacg
     2581 tgcaaaaacg ggccgacacc tctcatagga tagggcgcgt tcggcaatgc gatatcggtg
     2641 ccgtcgacct cgtagtcctt gccgcgaatc gtgagcgcga gccggtagcc ctgcggcacc
     2701 acgatcgagg tcggccagac ctcgacatcg agctcgaccg gctcgcccgg cttgagcggc
     2761 cgctcttcgt cgtgcgtgtg ccagggccgg tagggtttcg acagtttcgg atcgagcttg
     2821 cggtgcgagg cgcgcagcca gccgaggccg acgggcgtgc gcgggtcgtt cgagccgatg
     2881 aaggtcactt ccttgccggc gggatcgaac acgcgcagcg caaggaacag gtcggcgtcg
     2941 gtcgtttccg acgaaaccca cagtttcgcg gcgaccgggc cggtggcctc aagatccttt
     3001 gtcatcggcg gcgtcgagaa agtcagcccg tcgccctttg tttcatagcc gagcgtcgtt
     3061 gcctgcgatg gcgtgtccgg gctcagggtt tttccgtccg gctgcaggaa atattttgtc
     3121 cattgcgtgc gggcgagcgg ccattcgttc tcggcccgca gcacgaattt ctcgccagga
     3181 tggcggacgt tgagcgacac gcgcggctgc ttgtcccagc cggtattttc gcctttcagg
     3241 aagtgaccga agaacttttt ctgcagagcc tcgccgtagc ggctgtagaa gtgcgtgaag
     3301 tgcgtatcgc catgcacttc gagccatttc tgcctcgagc cggcacgcag ccagccctcg
     3361 aaattgccgc gtgtgtgcag gcccatgccg ccccagttcc cggcggagag caggggcgcc
     3421 tcgatatcct cgaatttcgc gatgcgcttg ctgtagtagt tgtcatagag acggcggcgt
     3481 ttcggctcgc ctggcgtgtc ggcaacgttt ttggcgagct cttccttcgg cagcgtttcg
     3541 ggccccgcca cgtattctcc cgtcaccacg ctctttttgc cgcgcgtgcc gactccatac
     3601 tgcacgctcg ccacctgacg cgggtgccag ctgttgagga aatcgctgag gatgccgccg
     3661 tggcggcaaa gctcgcgata gtagtcggac gaaccctccc agatgcacaa cgccgcgagg
     3721 tgagggggcc ggagggcgcc gaccgtccac tggttcatcg cgtaatagga aatgccgttg
     3781 atgccgacct tgccgctgct ccagggctgc gtgccggccc attcgacgca ctggtagagg
     3841 tcctgcgtct cgcgctcgca ccagacgtcg aggtagcccg gcgagtggcc ggcgccgcgg
     3901 gaatcgatgc gcaggcaggc atagccgtcc ggcacccatt tctccggatc gaccaattcc
     3961 cagttctggt acttgttgct cgagccttcc aggacctccg gcgcagcttt aaccagcctg
     4021 tcccaaaggc ccttgtagcc ttcctggaat gcgaggccct
//
LOCUS       DASUGB010000012         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1946735, whole genome shotgun sequence.
ACCESSION   DASUGB010000012 DASUGB010000000
VERSION     DASUGB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946735"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VFV87_00170"
     CDS             complement(<1..1054)
                     /locus_tag="VFV87_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102193.1"
                     /translation="MTDDRDPILDACLDEVLAGRRPPDLTARILQAWAARGIAGSRSD
                     PNVALPGPAIDSNVVLPAAPPVIAASSNGRLQETTPANGQPVVELRTTGSRSRSGRRK
                     SSFTAQAAMIAASVLAVAALAGVAYVASNQPQIANNDKPGKAPQQAVANNNTPAPQSK
                     QAEMPQVVTPPNKPLDWPQIVQTPSNPDSGSPQVVDNGTPNKPVVPLPAPEYGNPSPD
                     AEVVSFVNAELTQSWVEAGVKPSDPATDSEWCRRLFVRVLGRIPTVEELQSFLRDKSP
                     EKREQLVDRLLTDDAYNEEYARHWAVVWSNILIGRTGGRGESLADRDGLEQYLRTSLA
                     NNKPFNQLVHELVTATG"
     gene            complement(1060..1602)
                     /locus_tag="VFV87_00175"
     CDS             complement(1060..1602)
                     /locus_tag="VFV87_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5102194.1"
                     /translation="MNPAPAQQATGSPQPTTGFDPVHLIQTYQAAVWRYLRAMGCDPP
                     LAEDLTQETFLAILQRPFQDINPSATTAYLRKTALNLLISHHRRSGKVTAVENVEVLD
                     RTWTRWAGLDGGEAALDALRECLQRLTKRARTALEMRFRGDHSRAEIAQALEITEHGA
                     KNLMQRAKQQLRECIDGKLK"
     gene            complement(1883..>3134)
                     /locus_tag="VFV87_00180"
     CDS             complement(1883..>3134)
                     /locus_tag="VFV87_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102195.1"
                     /translation="SRRHFMAHLAGSGAAATAALSLGHSLRAHAAELKKNRKSAILLW
                     MSGGPSTMDIWDLKPGASTGGPFQQIATAGDVQISEHMPLTAKVMDKLSIIRSMSTRE
                     ADHQRGRYYMHTGYVPNPNIEHPSYGAVLSHELIDQRPDLEIPPFVSVGGGSEGPGFL
                     GMAWAPFVVNSNGQVRNLEMDVEQKRLEQRMWALAELEKGFISQNRGSSASDHAKILD
                     KTWKLMTSAQMDAFKVTKEPKEVQDRYGTDGFGRGCLMARRLVEAGVPFVEVDFGGWD
                     NHANIHNILSQGPQAKLPILDRGMSALVEDLDQRGLLQDTAIIWMGEFSRTPRINGNA
                     GRDHWARSWSVVVGGAGMKGGIAVGATNEDGTQVVTEPYTSQDVMASVCKALGISLET
                     TFTSKNGRPMKIANGGKVIKELFA"
BASE COUNT          526 a          978 c         1023 g          607 t
ORIGIN      
        1 agccggtcgc tgtcaccaac tcgtggacga gctgattgaa tggcttgtta ttcgccagcg
       61 acgtgcggag atactgctcc agaccatcgc gatcagccag cgattcgccg cggccccccg
      121 tgcggccgat caggatgttg ctccagacga cggcccagtg gcgggcgtat tcctcgttgt
      181 acgcgtcatc cgtcaggagg cgatcgacaa gctgctcgcg cttttcgggc gatttgtcgc
      241 gcaagaagct ctgcagctcc tcgaccgtgg gaatccggcc caagacgcgg acgaacaggc
      301 ggcggcacca ttcggaatcg gtcgccggat cagacggctt cacacccgcc tctacccagg
      361 actgagtcag ctcggcgttg acgaacgaga cgacttccgc atcgggcgac ggattgccgt
      421 actcaggcgc gggaagcgga acgacgggct tattgggcgt gccgttgtcg accacctgcg
      481 gcgagccgct gtcgggattg gacggcgtct ggacgatctg cggccaatcg agcggcttgt
      541 tcggtggggt aacgacctga ggcatctcgg cctgcttgga ctgcggggca ggcgtgttgt
      601 tgttggcgac ggcttgctgc ggtgcctttc caggcttgtc gttattggcg atttgcggct
      661 ggttcgaagc gacgtaagcg actccggcca gagccgcgac cgcgaggacg ctcgccgcga
      721 tcattgccgc ttgcgccgtg aaagaagatt tgcgacgtcc gctgcgcgac cttgagccag
      781 tcgtacggag ctcgactacg ggctggccgt tggccggcgt cgtttcttgc aagcggccgt
      841 tcgacgaggc tgcaatcacc ggcggggcgg ctggcagcac gacgttggaa tcaatggccg
      901 gccccggtag cgccacgttg ggatctgacc gcgatcctgc aatgccgcgc gcagcccacg
      961 cctgcagaat ccgcgcggtc aggtccggcg gacgccgacc agccaggact tcgtccaggc
     1021 aggcatcgag gatggggtcg cggtcgtcgg tcattccact cacttcaact taccgtcaat
     1081 acactcgcga agctgctgct tggcacgctg catcaggttt ttagcgccgt gttcggtgat
     1141 ctccagggcc tgggcgattt cggcccggga gtgatccccg cggaaccgca tctccagtgc
     1201 cgtcctggcc cgcttcgtca gccgctggag gcactcccgc agtgcgtcga gcgccgcctc
     1261 gccgccgtcc aggcccgccc agcgggtcca ggtgcggtcc aggacctcga cattctctac
     1321 ggccgtcact ttaccagacc ggcgatggtg cgaaatgagc aggttgagtg ccgtctttcg
     1381 caggtaagca gtcgtggcag agggattgat atcctgaaac ggccgttgga ggatcgccag
     1441 gaacgtctcc tgcgtcaggt cctccgccag tggcgggtca catcccatcg cccgcaagta
     1501 gcgccacaca gccgcctgat atgtctggat caggtggaca ggatcaaacc cggtggtcgg
     1561 ttggggagac cccgtggcct gctgggcggg agctgggttc atgtcccact acaagactaa
     1621 acgggccggt tttttgaatg tactcaggcc ggctggcgaa gaacgtaaga ataagggccg
     1681 ctgtgattta gagcgatggg ccggcgctag actcgcgacc gtgagctcaa cgagtttagc
     1741 gcccgcagcc ccattctaat cgttcgccgc gggacgggct attcgcaaga aatgccagcg
     1801 gccggggatt cgtgcgattc cccggccgca gtggttggtt ttgactcgcc gggaccaggt
     1861 ttggtccggc taaaaacatg gtttaggcga acagctcttt gatcaccttg ccgccgttgg
     1921 cgatcttcat ggggcggccg ttcttgctgg tgaaggtcgt ctcgagcgag atgcccaggg
     1981 ccttgcagac actggccatc acgtcctgcg aggtataggg ctcggtcacg acttgcgtgc
     2041 cgtcctcgtt cgtggccccg accgcgatgc cacccttcat gccggcgccg ccgaccacca
     2101 cgctccagct acgcgcccag tggtcgcggc cggcgttgcc attgatccgc ggcgtgcggc
     2161 tgaattcgcc catccagatg atggcggtgt cttgcagcag accgcgctga tcgaggtctt
     2221 caaccagggc gctcatgccg cggtcgagaa tcggcagctt ggcttgcggg ccttgcgaga
     2281 gaatgttgtg gatgttggcg tggttatccc acccgccgaa atcgacttcg acaaacggca
     2341 cgcccgcttc gaccagccgg cgagccatga ggcagccccg gccaaagcca tcggtgccgt
     2401 agcggtcctg cacctccttc ggctccttgg tgaccttgaa ggcgtccatc tgggcgctgg
     2461 tcatgagctt ccaggtcttg tcaagaatct tggcatgatc cgaagccgac gagccgcgat
     2521 tctgcgaaat gaaacccttt tcgagctcgg caagagccca catccgctgc tcgaggcgct
     2581 tctgctcgac atccatctcc aggttccgga cctggccgtt gctgttgaca acgaagggcg
     2641 cccaagccat gcccaggaaa cccggacctt cgctgccgcc gccgactgag acgaacggag
     2701 ggatttccag gtccggccgc tgatcgatca actcgtgcga gagaaccgca ccgtagctgg
     2761 gatgctcgat gttcggattc ggcacatagc cggtgtgcat gtagtagcgg ccgcgttgat
     2821 ggtcggcttc gcgggtgctc atcgagcgga tgatcgagag cttgtccatg accttggcgg
     2881 tcagcggcat gtgctcgctg atctgcacgt caccggctgt cgcgatctgc tggaacgggc
     2941 cgccggtgga ggcgccgggc ttcaggtccc agatgtccat cgtcgaaggg ccgccgctca
     3001 tccacagcag gatggccgac ttgcggttct tcttcagctc agccgcatgg gcccgcaggc
     3061 tgtgccccag cgagagggcg gccgtggccg cagccccgct gccggccaga tgagccataa
     3121 agtgacgacg cgac
//
LOCUS       DASUGB010000013         8870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1947085, whole genome shotgun sequence.
ACCESSION   DASUGB010000013 DASUGB010000000
VERSION     DASUGB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947085"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFV87_00185"
     CDS             <1..464
                     /locus_tag="VFV87_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102196.1"
                     /translation="FQHARTRPLSAEELIESWRVATGYAVWEASKEEKKKEGRYRPLD
                     GYMLRFFGQPNNGTGDFQGGLTEHLYLNNGPIGNLIVSGPGTLMESLSNKDQPVDERL
                     DRLFLQVLNRPPAAEERTKLAELISADKDPEERWRDAVWALMTCSEFRFNH"
     gene            710..1057
                     /locus_tag="VFV87_00190"
     CDS             710..1057
                     /locus_tag="VFV87_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102197.1"
                     /translation="MSKPAEAGSGESVFEGRYRKRMIKQARKVPVEDVRRFIDHGHLD
                     GPPQYFIVHSYGVELVRGWHPRHGVMELSMQDDVFHWACIEYLKSVGLVFDSEEAALD
                     YAAANVFPKILQQ"
     gene            1069..2337
                     /locus_tag="VFV87_00195"
     CDS             1069..2337
                     /locus_tag="VFV87_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102198.1"
                     /translation="MNPRCNPRDHLSRRALMKGLLGTAAGGVVMNWGGLTQTALAEEV
                     ARQQKYCIYLFMNGGASQFETFDMKPGRPTGGLFRPISTNVPGTQICELMPKMAQKMD
                     QIAVIRSMKTSEVDHPGGIYLMHTGYQPTANVRFPEVGSIVAKYQGREGADLPNFIKI
                     FGHGDAGAGFLGPKYLPFEISPEGQLPPFSASGLSPERELKRHELRAFIEDRFAAERK
                     SEVARMHRESYEAARRLQHARGAFKIDGEWEKYRDLYGDSAVGRRCLLARKLVEAGVP
                     FVEIGQSSYDSHADNFAWHKGLVPPMEHAWAGLIADLKDRGLFDKTLIIWAGEIGRTP
                     NINNRAGRDHYVRCWSAALAGCGVKGGLVYGASDEDGVEVKDNPVTEGDFFATIYQAL
                     SIDPKIENYAGVRPVPLAPFGSKVVGEMLA"
     gene            2445..3632
                     /locus_tag="VFV87_00200"
     CDS             2445..3632
                     /locus_tag="VFV87_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102199.1"
                     /translation="MNINNPKLLWQLNFEGPWPTGISFLGSGRRLAAANEGGLIYVWD
                     LPETPPEFEPPKNSERKAPDHVPVRWLQGHTNGVTRLVATLDGKTLVSASLDHTIRIW
                     EPETAATGTADAILDIDQRKSKAKRERNDEILTAPGVTVETLTSAHTLEAHEDWILSL
                     GASTDCRRLISGDNSSRVIVWDLAERKEVARWPGHPWNWIVAAALSADGQTAVVSEYR
                     YKRDDFDIPAAALRVYDTTTGEEKLDIVKTMFPKYKLDESSYGSAQVWRKFFAQGLIC
                     ADISPDGTLVALGQGGETDTGKVQLIEIASGKLLREVSGHQYGITDCKFSADGKLMLS
                     TGRDTTLRICQVEDGKEVAALGNPRGGQFKDWFNNLAISPDQKTVAATDIAGLVHVWA
                     LEG"
     gene            3674..6664
                     /locus_tag="VFV87_00205"
     CDS             3674..6664
                     /locus_tag="VFV87_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5102200.1"
                     /translation="MPKPIATLLLLIATALVCPAAASAQQPSATARRAEFGLARRIPW
                     TTSRLVGSPEPPPKYIAQRVFPSLAFETPGEMAAIPGTNRLVVVEVMGKLYSFENQPE
                     AESVERELFADLTAIDPQFYRVYGLTFHPRFAENRYCYISYVLQPKTPDGTRVSRFKV
                     TDTDPPRVDLASEETVITWMAGGHNGAHLQFGPDGCLYISTGDGGDSFPPDGRNTGQD
                     NSDLLANILRIDVDHPDAERLYRIPADNPFVGTPNCRGEIWAYGLRNPWKMCFDPADG
                     SLWVGDVGWEMWEMIYRIERGGNYGWSIVEARQPVHQERERGPTPILPPTVEHSHIEA
                     RSITGGYFSRTPRLPELKGAYIYGDYVTGKVWALRHDGGKVTWREELVDTPLQIVSFG
                     LDPTGEVYIVDHPGGGIYRLAPNPRQGANRDFPTKLSETGLFASTADHQPAPGVIPYS
                     INAEPWADGTLAERFVAVPGETQLGVFQKSDIQIGYIAGDWSFPSGGVLAKTVSIELE
                     PGNPASRRRLETQVLQYDVDTWRAYNYIWTDEQTDALLAPDQASDRALEIKGPDAPGG
                     RERQTWHHASRTECILCHTTRAGSIHGFRPEQLDRLHDYGGTISDQLRTLDHIGLFAE
                     PLPENIEAWPDPHDQKADLTARARAYLHVNCGHCHRRGGGGSSFFDVQYQFPLEKTSL
                     LGTRPTQGTFGIHDAQIVAPGDPYRSVLYYRISKLGHGRMPQFGSQVVDLRGAKLIHD
                     WIVSLPAAGDQTAAGSVQRMRADERAALETFKSATADDANRTAALDRLLASASGGLQL
                     LLALDDSRVGDAARQLAIDRGTSHSDPILRDLFERFIPEDQRIQRLGTVILPDAILAL
                     AGDANRGRDLFFQAAGVQCRNCHRIGNEGKQLGPDLTTIGKKLDRAKLLESILQPSLA
                     IDPQFATYLVETTDGLVHTGLLLRRTDAEVALQSAEGKEIAVSAAEIERFAPQQKSLM
                     PDLLVQDLTAQQLADLLQFLAELQ"
     gene            complement(6707..6789)
                     /locus_tag="VFV87_00210"
     tRNA            complement(6707..6789)
                     /locus_tag="VFV87_00210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6754..6756),aa:Leu,seq:caa)
     gene            7090..8616
                     /locus_tag="VFV87_00215"
     CDS             7090..8616
                     /locus_tag="VFV87_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102201.1"
                     /translation="MNDHSFLLLWDLLMSTYRCASLSFAHLFLGLVLAAPAAAQFEAL
                     AAKVPSTANAIVLLDGQKVMSSPLAEKEGWKEKYEAAFASGVVTIAPDTRHMVLASQM
                     DYQYMRPLWEVAIGDFADSRSVAEIARRTKGTIDSLGGLQAVALSDDSYAVEFSPTRL
                     GVMAPGNRQTVSRWVSDVRDRDQPQLSPYLKGTLTASNTSQIVIAFDLQDAVPPHILR
                     DKLAASSSLAGKMVDLDAAAAALASIRGLVLEVAITDGSYGRLMIHFGSDASVLAPYA
                     KPILLEALGNMGAMIDDLAEWKVTTEPTRFTFQGMLSADGRKRVFSLIDHPIAALVAS
                     DASKDQSTMPEQSKMAQATLQYFRSIVELQDDLREKSKDAKTFGQNALWFDNWARKID
                     RLPILNVDPDMLAYGRYVSSRMRDISAALKGIGIQSAAKEAQVYQQFSTSASGYGGYW
                     GGEYSYYTQWNNVGGQRRAISAQERAQGATTARTIAAEMENQMASVRQAMTQKYQINF
                     "
     gene            8651..>8870
                     /locus_tag="VFV87_00220"
     CDS             8651..>8870
                     /locus_tag="VFV87_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102202.1"
                     /translation="MGHRLRRSEVLAAMLLAFAAAARADDLAAGKEAGYFPLPESKGG
                     WRTIEDREAIRKAAGMDPAKLDELKEWLL"
BASE COUNT         1677 a         2984 c         2757 g         1452 t
ORIGIN      
        1 ggttccagca cgcccgcacg cggccgctct ccgccgaaga gctgatcgaa agctggcgcg
       61 tcgccaccgg ctacgcagtc tgggaggcga gcaaagagga gaagaaaaag gaaggccgct
      121 atcgcccgct cgacggctac atgctgcgat tcttcggcca gcccaacaat ggcaccggcg
      181 attttcaggg cggcctgacc gagcacctct acctcaacaa cggcccgatc ggcaacctca
      241 tcgtcagcgg gcccggcacg ttgatggaat cgctcagtaa taaggaccag ccggttgatg
      301 agcgacttga ccgcctcttc ctccaagtcc tcaaccgccc gcccgccgcc gaggaacgca
      361 ccaaactcgc cgagctgatt tccgccgaca aagatcccga agaacgctgg cgcgacgcgg
      421 tgtgggcgct gatgacgtgc agtgaattcc gatttaacca ctaactcgtt taccccgcag
      481 ccgaaggcgc aggtggaagt gccaacaaac ctgacaacaa atgcagccgg cttcagccgg
      541 cttaccccgt taggcccggc cttcaggccg ggcagctcgg ggcgtattga tttggttgcc
      601 agccggcttg tgtccggctt cagccttggt tcggggaagc cggctaaagc cggctggagg
      661 agaatgagtt ttgtggaccg cccagccccg gctaaagccg gggcctaacg tgagcaagcc
      721 ggctgaagcc ggctcaggtg aatcggtgtt cgagggacgt tatcgaaagc gaatgatcaa
      781 acaggcgcgc aaggtgcccg tggaagatgt gcgccgattt atcgatcatg gtcatttgga
      841 tggtccgccc cagtacttca tcgtgcattc atacggcgtc gagctggtgc gcggttggca
      901 cccaagacac ggcgtaatgg aattgtcgat gcaggatgac gttttccatt gggcatgcat
      961 cgagtacttg aagtcagttg gtctggtctt tgattcggaa gaggccgcac tcgactacgc
     1021 cgcggccaac gtgtttccaa agattttgca acagtaggag catcagtcat gaacccccgc
     1081 tgcaaccccc gcgaccacct ttcccgccgc gccctcatga aaggtttgct cggcaccgcg
     1141 gcgggcggcg tcgtaatgaa ctggggcggg ctgacgcaga cggcgctggc cgaggaggtc
     1201 gcccggcagc agaagtactg catctacctg ttcatgaacg gcggggcgag ccagttcgag
     1261 acgtttgaca tgaagccggg gcggccgacg gggggactgt tccggccgat ctcgaccaat
     1321 gtgcccggca cgcagatttg cgagctgatg ccgaaaatgg cccagaaaat ggaccagatc
     1381 gccgtcatcc gctcgatgaa gacgagcgaa gtcgatcatc ccggcggcat ttacttgatg
     1441 cacaccggct atcagcccac ggccaacgtg cggttcccgg aggtcgggtc gatcgtcgcc
     1501 aagtaccagg gccgcgaagg ggccgacctg cccaacttca tcaagatttt cggccacggc
     1561 gacgccgggg cgggcttcct cgggccgaag tatctgccgt tcgagatcag ccccgaaggg
     1621 caactgccgc cgttctcggc ttccggcctc tcgcccgagc gggagctgaa gcggcacgag
     1681 ctgcgggcct ttatcgagga ccgcttcgcc gccgagcgaa agagcgaagt cgcccggatg
     1741 caccgcgagt cgtacgaagc ggcccgccgg ctgcagcatg cccgcggggc gttcaagatc
     1801 gacggcgaat gggagaagta ccgcgatttg tacggcgaca gcgccgtcgg ccgccgctgc
     1861 ctgctcgccc gcaagctcgt cgaggcgggc gtgccgttcg tcgagatcgg ccagagcagt
     1921 tacgactcgc acgccgacaa tttcgcctgg cacaaggggc tcgtgccgcc gatggagcac
     1981 gcctgggccg ggctgatcgc cgacctgaag gaccgcggcc tctttgacaa gacgctcatc
     2041 atctgggccg gcgaaatcgg ccgcacgccg aacatcaaca accgggccgg ccgcgaccac
     2101 tatgtccgct gctggtccgc tgccctcgcc ggctgcggcg tgaaaggcgg cctggtctat
     2161 ggcgcgagcg acgaggacgg cgtcgaggtc aaggacaacc ccgtcaccga aggggacttc
     2221 ttcgccacga tctaccaggc cctttcaatc gaccccaaga tcgaaaacta cgccggcgtc
     2281 cgccccgtgc cgctcgcgcc gtttgggtcg aaggtggtgg gggagatgct ggcatagtag
     2341 taggcacgca ccgcgtgccg catcgtagcc cgtagcgcaa gcgagggtaa tcgacgtcga
     2401 tgatgtaata acctccataa ccctcgctcg cgctacgggc tacgatgaac atcaacaacc
     2461 ccaagctcct ttggcaactg aacttcgagg gcccgtggcc gacggggatt tcgttcctcg
     2521 gcagcgggcg gcgcttggct gcggcgaatg agggcgggct gatttacgtc tgggacctgc
     2581 cggaaacgcc cccggagttt gaaccgccga agaatagcga gcggaaagcg cccgaccatg
     2641 tgccggtccg ttggctgcaa gggcacacca acggcgtgac gcggctggtt gcgacgctcg
     2701 acggcaagac gctcgtttcg gccagcctcg accatacgat ccgcatttgg gagccggaaa
     2761 cggccgcgac tggaacggcc gatgcgatcc tcgacatcga tcagcggaag agcaaggcca
     2821 agcgcgagcg gaacgatgag atcctcaccg cgcctggcgt gacggtggaa acgctcacct
     2881 ccgcgcacac actcgaagcc catgaagatt ggatcctgtc gctcggcgcg agtaccgact
     2941 gccgccggct gatcagcggc gacaactcct cgcgggtcat cgtctgggac cttgcggaac
     3001 gtaaagaagt cgctcgctgg cccggccatc cgtggaactg gatcgtcgcg gcagcgctct
     3061 ccgccgacgg ccagacagcc gtcgtcagcg agtaccgtta caagcgcgac gatttcgata
     3121 taccggctgc ggccctgcgc gtgtacgaca cgacgaccgg cgaagagaag ctcgacatcg
     3181 tcaaaacgat gttccccaag tacaagctgg acgaatcgtc gtatggctct gcccaggtgt
     3241 ggcgcaagtt tttcgctcag gggctgatct gcgcggacat ctcgccggac ggcacgctcg
     3301 tcgcgctcgg ccagggcggc gaaacggaca cgggcaaggt gcaactcatc gagatcgcca
     3361 gcggcaagct cctccgcgaa gtcagcggcc accagtacgg catcaccgat tgcaaattct
     3421 ccgccgatgg gaagctgatg ctctccaccg gccgcgacac cacgctccgc atctgccagg
     3481 tggaagacgg caaagaagtc gccgccctcg gcaacccccg cggcggccag ttcaaggact
     3541 ggttcaacaa cttggcgatt tcgccggacc agaagacggt cgcggccacc gacatcgcgg
     3601 ggcttgtgca tgtgtgggcg ctcgaaggat gatggtgcgg caccacgctc acgcaagaca
     3661 acctccgccc gccatgccga agcccatcgc aacgctactt ctcctgatcg caacggcgct
     3721 ggtctgccct gctgccgcga gcgcgcagca gccgtccgcg acggcccgcc gcgcagaatt
     3781 cggcctggcg cgccgcatcc cctggacaac ctcccgcctc gtcggctcgc cggagcctcc
     3841 gccgaaatac attgcccagc gcgtctttcc ctcgctggcc ttcgaaacgc cgggcgaaat
     3901 ggccgcgatt cccggcacca atcggctggt ggtggtcgaa gtcatgggca agctgtattc
     3961 attcgagaat cagccagagg cggagagcgt cgagcgcgag ctgttcgcgg acctgacggc
     4021 gatcgacccg cagttctacc gcgtctatgg cctgacgttt cacccgcgtt tcgccgagaa
     4081 ccgctactgc tacatctcct acgtcctgca gcccaagacg cccgacggga cgcgggtttc
     4141 gcggttcaaa gtgaccgaca ccgatccgcc ccgcgtcgat ctcgcctccg aagagaccgt
     4201 catcacctgg atggccggcg ggcacaacgg ggcccacctg cagttcggcc cggacggctg
     4261 cctctatatt tcgaccggcg atggcggcga cagtttcccg cccgacggcc gcaacacggg
     4321 gcaggataac agcgacctgc tggcgaacat cctgcggatc gacgtcgacc atcccgacgc
     4381 cgaaagactc tatcgcatcc cggcagacaa tcccttcgtc ggcacaccca actgccgcgg
     4441 cgaaatctgg gcctatggcc tccgcaatcc ctggaagatg tgcttcgacc cagccgacgg
     4501 ctcgctgtgg gtcggcgacg tcggctggga aatgtgggag atgatttacc gcatcgagcg
     4561 cggcggcaat tacggctgga gcatcgtcga agcccgccag ccggtgcacc aggagcgcga
     4621 gcgcggcccg acgccgatcc tgccgccgac cgtggagcac agccacatcg aagcccgctc
     4681 tatcaccggc ggctatttct cgcgaacgcc gcggctgccg gaattgaagg gggcctatat
     4741 ctacggcgat tatgtcacgg gcaaggtttg ggccttgcgt cacgacggcg gcaaggtcac
     4801 ctggcgcgag gagctcgtcg atacgccgct gcagatcgtc agcttcgggc tcgatccaac
     4861 tggcgaggtc tatatcgtcg atcatccggg cggcggcatc tatcgactcg ctcccaatcc
     4921 aaggcaaggc gccaaccggg acttccccac gaagctgagt gaaacggggc tcttcgcttc
     4981 cacagccgac caccagccgg ccccaggcgt gattccctac agcatcaacg ccgagccctg
     5041 ggccgacggc acgctggccg agcgcttcgt cgccgtgcct ggcgagacgc aactgggcgt
     5101 tttccagaag tccgatatcc agatcggcta cattgccggc gattggtcgt tccccagcgg
     5161 cggcgtgctg gccaagacgg tttcgatcga gctggagccc ggcaatccgg cttcgcgccg
     5221 ccggctggag acgcaggtcc tgcagtacga tgtcgatacg tggcgcgcct acaactacat
     5281 ctggaccgac gagcagaccg acgcgctgct cgcgcccgat caggccagcg accgcgccct
     5341 ggaaatcaag ggccccgacg ctcccggagg ccgcgagcga caaacgtggc accacgccag
     5401 ccgcaccgag tgcatcctct gccacaccac gcgggccggc tcgatccacg gcttccggcc
     5461 ggagcagctg gatcggctgc acgactacgg cggcaccatc tccgatcagc ttcgcacgct
     5521 cgaccacatc ggcctcttcg ccgagccgtt gccggagaat atcgaagcct ggcccgatcc
     5581 gcacgaccaa aaagccgacc tgaccgcgcg ggcccgcgcg tacctgcacg tcaactgcgg
     5641 ccactgccac cgccgcggcg gcggcgggtc gtcgttcttc gacgtgcagt accagtttcc
     5701 gctcgaaaaa acctcgctgc tcggcacgcg gccaacgcag ggcacgttcg gcatccacga
     5761 cgcgcaaatt gttgccccgg gggaccccta tcgctccgtc ctctattacc gcatctccaa
     5821 gctcggccac ggccggatgc cgcagttcgg ctcgcaagtc gtcgatcttc gcggcgcaaa
     5881 actgattcac gactggatcg tcagcttgcc ggcggccggc gatcagacgg cggcggggtc
     5941 cgtccagcgc atgcgggccg acgagcgggc tgcactcgaa acgttcaaat cagcaacggc
     6001 cgacgacgcc aaccgcacag ccgcactcga ccggctgctc gcctccgcca gcggcgggct
     6061 gcaattgctc ctggctctcg acgacagtcg cgtaggagac gccgcacgcc aactggcaat
     6121 cgaccgcggc acatcacaca gcgaccccat ccttcgcgat ctcttcgagc gcttcattcc
     6181 cgaagaccag cgcatccagc gcctgggcac cgtcattctg ccggacgcca tcctcgccct
     6241 cgcgggcgac gccaaccgcg gccgagacct cttcttccaa gccgcgggcg tccagtgccg
     6301 caattgccac cggatcggca acgaaggcaa gcagctcggg cccgatttga cgacgatcgg
     6361 caagaagctc gaccgcgcga agctgctgga aagcatcctg cagccctcgc tggccatcga
     6421 cccgcagttc gcgacctacc tggtcgaaac caccgacggc cttgtccata ccggccttct
     6481 gctccgccgg accgacgccg aagtcgccct ccaatcggcg gaagggaagg agatcgccgt
     6541 ttctgcggcc gaaatcgagc gtttcgcacc ccagcaaaag tcgctcatgc ccgacctgct
     6601 cgtccaagat ctgaccgccc agcaactcgc cgacctgctc caattcctgg ccgagcttca
     6661 atagtctgcg gtgcacgccg aaggccgctg cgctctgacg atccagtacc agaggtggga
     6721 cttgaaccca cacgcccttg cgggcactgg attttgaatc cagcgcgtct gccattccgc
     6781 cactctggct cgatgatcag tgcagactca gtttgctggt gaaggttcgg ggcgtcaagg
     6841 ctgctaatga ggaaggggtc ggcgcgacgc gcgaatcgcc ggccagccca ctgacaacgg
     6901 ctgcaagcaa accatcagac acttgctgcc gcatgggttc cgagttaggg tatcaccagc
     6961 ctgcgaatca gggcacaaac ggtgtgggga atgggcgccg cccgttccaa gacctctcga
     7021 gccgcgctcg tcttccctcg gtgcccattc gccggcggcc tcctcgcgca gtttcgcgcc
     7081 gcacacccat tgaatgacca ctcgttcctg ctcctctggg atttgctgat gtccacctac
     7141 cgctgtgctt ctttgtcatt tgcccacttg ttcctgggac tcgtccttgc cgcgccggct
     7201 gcggctcaat tcgaagccct ggccgccaag gttcccagca cggccaatgc gattgtgctg
     7261 ctggacgggc aaaaggtgat gtccagcccg ctggccgaga aggaaggctg gaaagagaag
     7321 tacgaagcgg cgttcgcgtc cggcgtggtc acgatcgccc ccgacacgcg gcacatggtg
     7381 ctggcgtccc agatggacta tcagtacatg cggccgctgt gggaagtcgc catcggcgac
     7441 tttgccgact cgcgctcggt ggccgagatc gcccgccgca ccaaggggac gatcgattcg
     7501 ctgggcggcc tgcaagcggt ggccctgagc gacgattcgt acgccgtcga attctcgccc
     7561 acgcgcctgg gcgtcatggc gcccggcaat cgccagacgg tctcgcgctg ggtcagcgac
     7621 gtgcgcgacc gcgaccagcc gcagctttcg ccctacctga aagggacgct gaccgcctcg
     7681 aacaccagcc agatcgtcat tgccttcgac ctgcaggacg ccgtgccgcc gcacatcctc
     7741 cgcgacaagc tcgccgccag ctcctcgctc gccgggaaaa tggtcgatct cgacgccgca
     7801 gccgcggccc tggccagcat tcgcgggctg gtgctggaag tggcgatcac cgacggctcc
     7861 tacggccgct tgatgatcca cttcggctcc gacgcttcgg tcctcgcccc gtacgccaag
     7921 ccgatcctgc tggaagccct gggcaacatg ggggcgatga tcgacgacct ggccgagtgg
     7981 aaggtcacca ccgagcccac gcggttcacg ttccagggaa tgctcagtgc cgacggccgc
     8041 aagcgggtct tttcgctcat cgaccacccc atcgccgccc tggtcgcgtc ggacgcctcg
     8101 aaggaccagt ccacgatgcc cgagcagagc aagatggccc aggccacgct gcagtatttc
     8161 cggtcgatcg tggaactgca ggacgatctg cgcgagaagt caaaggacgc caagacgttc
     8221 ggccagaacg ccctgtggtt cgacaactgg gcccgcaaga tcgaccgcct gccaatcctc
     8281 aacgtcgatc ccgacatgct ggcctatggc cgctatgtct cctcgcggat gcgcgacatc
     8341 tcggcagctc tgaaggggat cggcatccag tcggccgcca aagaggccca ggtctatcag
     8401 cagttctcga ccagcgccag cggttatggc gggtattggg ggggtgagta ctcgtactac
     8461 acgcagtgga acaacgtcgg cggccagcgg cgggccatca gcgcccagga gcgggcccaa
     8521 ggcgccacca ccgcccgcac cattgctgcc gagatggaaa accagatggc cagcgtccgc
     8581 caggcgatga cgcagaaata tcagatcaac ttctgatgtc cctctggcgc cgtccgcagg
     8641 agagatgccg atgggccaca ggcttcgacg cagcgaagtt ctcgcggcca tgctgctggc
     8701 tttcgctgcc gcggcgcggg cggacgatct ggctgcgggg aaggaagcgg gttatttccc
     8761 gctgccggaa tcgaaggggg gctggcggac gatcgaggac cgcgaggcga ttcgtaaggc
     8821 ggctggcatg gacccggcga agctggacga gctgaaggag tggctcctgg
//
LOCUS       DASUGB010000014         9596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_557662, whole genome shotgun sequence.
ACCESSION   DASUGB010000014 DASUGB010000000
VERSION     DASUGB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557662"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..479
                     /locus_tag="VFV87_00225"
     CDS             219..479
                     /locus_tag="VFV87_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102203.1"
                     /translation="MSLTVELPPQLAAELAAEAARRQMPLEDYAVQVLAGHSGARAQT
                     MSGAELVRYWHEAGIVGTRTDIADAPAHARQLRDQAQRRERG"
     gene            483..863
                     /locus_tag="VFV87_00230"
     CDS             483..863
                     /locus_tag="VFV87_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5102204.1"
                     /translation="MKLVDTDVLIDIQRGHPPAVAWFAGLSELPLVSGFVVMELVQDA
                     RNASEVNQALKLVAPLSVVWPAEVDCNRALADFAAYHLTHGLGLIDAMIAATAVGRSL
                     TLYTFNSKHYRSVPGLLFAEPYTR"
     gene            complement(876..3209)
                     /locus_tag="VFV87_00235"
     CDS             complement(876..3209)
                     /locus_tag="VFV87_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5102205.1"
                     /translation="MSNASPRRQRPYRGGSLALPPNFFSQLWATARRPDVLLRLGLWI
                     AACMILWAVTGAGIPPFAFRVGDVPQRKIVARVNFELPNVAETEKRKAEARRTAEPVY
                     INNVRLLEETRQGLTNKVSQLVQAESFDKVDQTLWAEFSPPNSERPAGDDKQRFDALK
                     AYFTAGMDSGRFDEAVKRALEPIQRSGVLKEKDPHDQGSELSLLVREANGERPSHRAT
                     IDEVRIDKVKASLRQRLLDELKVLDASMMASTSVVADLTYAWLESHLPATLSYDQEAS
                     IKEADRAESEIEPVMTAFETNVTELAPGGKPLTEDSLLLLQKEYEAWVATLNWEDQLA
                     RSFSMFGMYSAVTILCGFYIYHRRPKLLADLRRYVTLLATVVVTVTLCVLLSGGPHAE
                     IIPLLLFGMTAAIAYEKELALLLAASLALIVAFSRSQGLAEFVILVSSVAAAILMLRR
                     IRSRTKLIYVGAFAGLVVVLTTIGVGTLVGRAFGISSLAIEQLSPAYRESFAVSLLAG
                     AAWFGVCALLAGLLMTGLLPFVERMFDVQTDISLLELGDVQHPLLQELVRRAPGTYNH
                     SINVASIAETAAEAIGANGLLVRVGAYFHDVGKMLKPSYFVENQGPEGNKHEALQPAM
                     STLVIIAHVKDGADLARQHHLPQPIIDFIEQHHGTTLVEFFYRREAQRLKDDPDAGDL
                     DETSFRYPGPKPQTKEGGVLMLADVVESASRALVDPTPSRIESLVHDMAMKRLLDGQF
                     DESGLTLSELRIIEDSLVKSVTAVYHGRVKYPEHQTV"
     gene            complement(3296..4258)
                     /locus_tag="VFV87_00240"
     CDS             complement(3296..4258)
                     /locus_tag="VFV87_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5102206.1"
                     /translation="MTEATIPLVDSHRVLTLFGARDQHVRTIRDALGVSITHRDGEIR
                     VAGEEGAVARATAALTQLSTQLDEGKEIGLDHVADALAHVTGKRAVALQQSVDVIQAA
                     RRIAPRTQGQARYIDSIRKHDITFAVGPAGTGKTYLAVALAVEALKHHQMRKIVLVRP
                     AVEAGESLGFLPGDLRAKINPYLRPLLDALHEMIDFDQMKKYMEQEVIEVVPLAYMRG
                     RTLNEAFIILDEAQNTTIAQMKMFLTRMGQGSKIVVSGDTTQIDLPRPSASGLIDALG
                     RLRDIEGVSVVQLNKSDIVRHRLVQDIVRAYEEEGQKAAMRKGR"
     gene            4819..5730
                     /locus_tag="VFV87_00245"
     CDS             4819..5730
                     /locus_tag="VFV87_00245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5102207.1"
                     /translation="MPAKRSSTSAAAIDGGPALNQMLQGDALEVLRTWPEGLVDCVVT
                     SPPYWRQREYAASREQVGQEASPAAYVERLTEIFRECRRVLKAAGSLWLVIGDKYDDG
                     RQLGLPWRVALSLIDDGWILRADCIWHKPNAMPSSTKSRPTTDHEYIFFFTQSPDYYY
                     DADAIREPHVTFSDKSRMRGGRGHFGRRGGTPEAGKNGGSQNLHDGRWDQAFHPLGRN
                     KRTVWSIPLSKFREAHFAVFPESLVETCIRAGCPAGGVVLDPFLGSGTTAVVAQRLGR
                     SYLGIDCSAEYCEMARRRIGNGQPLFD"
     gene            5843..7369
                     /locus_tag="VFV87_00250"
     CDS             5843..7369
                     /locus_tag="VFV87_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5102208.1"
                     /translation="MIRRLHYLAAVALIALAAAAGAQELPPGIDEYVEAARSKWQVPA
                     LSIAVVKDGKVVHLRGYGPRSIGGSEPVDGQTVFRLASISKTFTAATAALLVEEGKLT
                     WDDPIRKHVPEFELHDRYLTEHVSLRDCLSHRVGLETGDIVARRGDLSRDEILARLRF
                     LQPYSPFRGKFKYNNLMYVAAGETVARAAGQSWEDFLSRRLLVPLGMRSTTPTFASLH
                     ADNLATAYRLHDGELQAVTTAPIIDAVAPAGSVHSSAADMARWLQFWLAEGELDGQRL
                     LQRGTVREMLAMHSTVPIERSDDSNIYRAKFYGWGLGWSVLDYRGRKIHTHAGGSGTF
                     IGFMPEAGIGVVVLTNLEFTNLGGMLMYDVFDAYLLDRERAWNRDQWPLWLAVDEPPE
                     ITGDKARAKLDAMRKAGTSPTLSLAQLAGKYRSDLYGEIEILDGDGGLQLKLGRNPPV
                     KLQHWQDDTFVSPGPEADAPWFDWLLKFRITAGECEALDIERIGWDEPLPTFHRAADS
                     "
     gene            7420..8490
                     /locus_tag="VFV87_00255"
     CDS             7420..8490
                     /locus_tag="VFV87_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102209.1"
                     /translation="MRLTHYRSVASIAAWFAAVGAVRAAEPSTIPGKRLPEIKEPVLL
                     GTAEADAIAAALQVFPPDNAWNTDISRWPLHPNSAAIVASIGNDKPLRYNTDMSYVFV
                     PPGQKLVDVAIVEYPDESDKGPYPVPDNTPIEGWPAWHRRDAEQKLTLEEVQRDKLNQ
                     GGDRHAIVVDPYAGKLYEFYQMKRTDPASGEQASGGRRLPEPRWQCACAAIFNLRTND
                     LRQGGWTSADAAGLPIYPAVIRYDELAAGKVNHAMRVTVRSTRRAYVAPATHFASRKT
                     DENLPRMGERLRLKAGYDIRGFSPEVQTILKGLKQYGMLVADNGLEWAISCAPDERIP
                     VLHEELRRVKGSAFEVVRAPER"
     gene            complement(8512..8691)
                     /locus_tag="VFV87_00260"
     CDS             complement(8512..8691)
                     /locus_tag="VFV87_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102210.1"
                     /translation="MNDRDDQRTSHDSKRDSGAKPAPRYACPVCGGHLLEIRGKLQCE
                     RCHTIIETCCEGGPP"
     gene            complement(8688..9182)
                     /locus_tag="VFV87_00265"
     CDS             complement(8688..9182)
                     /locus_tag="VFV87_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102211.1"
                     /translation="MPCPCGRVVKVPRLSELRRQAGQAAFQVSAIDRIRSMLQSRCEA
                     PGRECALSGIVTSDVMMFTVVCEKPYSTGYSWWWLFVIGLWSLPLSIFGYIIATRESG
                     EAQGRELTVTVPLRIAAHCQADVRKFSPLQLANLLYTVPLYAALLDEYPYAEVSVGQI
                     AGRR"
     gene            complement(9285..>9596)
                     /locus_tag="VFV87_00270"
     CDS             complement(9285..>9596)
                     /locus_tag="VFV87_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102212.1"
                     /translation="GRPVRPYEEWRFPYRPFSVPYDAWGPPFGGLGGGNGFYPYSGGG
                     FGGRFGGGFGPIGPGFPGGGGGFRGQPPVNFAQPWLDGHYPQYDLNDRSPYWKPYLPG
                     P"
BASE COUNT         1722 a         3047 c         3138 g         1689 t
ORIGIN      
        1 ctggagacat ccggcttcta tcctgaacgc gacagtcgta agctgccacc cagcgcgcat
       61 gatcgactcg caacagcgca agcccgcatt ggcgatcgcc tttgggacct cgattcacgg
      121 ttagccgcac tcttggacgc cgagggcaac aaggaaactt gaacgtcaaa tgacgactgc
      181 cggtctttga tgtagaattg ggctgtagga gcttgcttat gagcctgact gtcgagttgc
      241 cgcctcagct cgctgccgaa ctcgcggcag aggcagcccg tcgccagatg ccgctagagg
      301 actatgccgt gcaggtgttg gccgggcatt ccggagcgag ggcacagacc atgtcgggcg
      361 ccgagctcgt gcggtattgg cacgaagccg gcatcgtggg cactcggacg gacattgccg
      421 atgcgcccgc acatgcccgc caacttcgcg accaggccca gcggcgcgag cgcgggtaat
      481 cgatgaagct cgtcgatacg gatgtgctga tcgacatcca gcgcggccat ccgccggcgg
      541 tggcctggtt tgcggggctg agcgagctgc cgctcgtctc tgggttcgtt gtcatggagc
      601 tggtgcaaga cgcccgcaat gcgagcgaag tgaatcaggc cttgaagctg gtcgcgccgc
      661 tgtctgtcgt ctggccggct gaagttgatt gcaatcgcgc tctggccgat ttcgccgcgt
      721 accatcttac gcacgggctc gggctgattg acgcgatgat cgccgcgact gcggtggggc
      781 gatcgctgac actctacacc ttcaattcaa agcactatcg cagcgtgccc ggactattgt
      841 ttgccgaacc gtacacgcgc taagagcgcg cggtgctaga ccgtctgatg ctcggggtat
      901 ttcacccggc cgtgatagac ggcggtgacg cttttgacga gcgagtcttc gatgatccgc
      961 agttcgctga gcgtgaggcc gctttcgtcg aattgcccgt ccagcagccg cttcatggcc
     1021 atgtcgtgga ccaggctctc gatccgcgag ggagtgggat cgacgagggc ccggctggcg
     1081 ctttcgacga cgtcggccag catcagcacg cccccttcct tggtctgcgg cttggggccg
     1141 ggatagcgaa acgaagtttc gtcgaggtcg cccgcatcag gatcgtcctt cagccgctgc
     1201 gcctcgcggc ggtagaagaa ctcgacgagc gtcgtgccgt gatgctgctc gatgaagtcg
     1261 atgatcggct gcgggaggtg gtgctggcgg gccagatcgg cgccgtcctt cacgtgggcg
     1321 atgatcacga gggtgctcat ggccggctgc agggcttcgt gcttgttccc ttccgggccc
     1381 tggttctcga cgaaatacga cggcttgagc atctttccga catcgtggaa ataggccccc
     1441 acgcggacga gcaggccgtt agccccaatc gcctcggcag ccgtttcggc gatcgaggcg
     1501 acgttgatcg aatggttgta ggttcccggc gcgcggcgaa cgagctcctg caacagcgga
     1561 tgctgcacgt cgcccaattc cagcaggctg atgtcggtct gcacgtcgaa catccgctcg
     1621 acgaacggca agaggcccgt catgagcagc ccggccagca gggcgcacac gccgaaccaa
     1681 gcggccccgg ccaggagcga caccgcgaac gactcgcgat acgcgggcga aagctgctcg
     1741 atcgccaggc tgctgatgcc gaaggcccgg ccgacgaggg ttccgacgcc gatcgtcgtc
     1801 agcaccacca ccaggccggc gaaggccccg acgtagatca gcttcgtgcg gctgcgaatg
     1861 cggcggagca tgagaatcgc cgcggccacg gacgaaacca gaatcacgaa ctccgccagc
     1921 ccctgtgagc gcgagaacgc gacgatcagc gccaggctgg ctgccaagag cagcgccagc
     1981 tctttctcat aggcgatggc cgccgtcatg ccaaataaca gcagcggaat gatttcggcg
     2041 tgcggtccgc cgctgagcag cacgcacagc gtcacggtga cgacgaccgt cgccaacagc
     2101 gtgacatagc ggcgcaggtc cgcgagcagc ttcggccggc ggtgatagat gtagaatccg
     2161 cacaggatgg tgacagccga atacatgccg aacatcgaga agctgcgggc gagctggtct
     2221 tcccaattca gtgtagcaac ccaggcctcg tattcctttt ggagcagcag gagcgagtct
     2281 tcggtcagcg gctttccgcc cggggccaat tccgtcacgt tggtttcaaa ggccgtcatg
     2341 accggttcga tttcggattc ggcccggtcg gcttccttga tcgaagcctc ctggtcgtag
     2401 gagagcgtcg cgggcagatg cgattcgagc caggcgtagg tcagatcggc cacgaccgag
     2461 gtgctggcca tcatgctggc gtccagcact ttcaattcgt cgagcaggcg ctggcggagc
     2521 gaggctttca ccttgtcgat ccgcacttcg tcgatcgtcg cccggtgcga cggccgttcg
     2581 ccgttggcct cgcggaccag gagcgacagc tcgctcccct ggtcgtgcgg gtccttctcc
     2641 ttgagcacgc cgctgcgctg aatcggctcc agggcccgct tgacggcttc gtcgaagcgg
     2701 ccggagtcca taccggccgt gaaataggct ttcaatgcgt cgaaacgctg cttgtcgtcg
     2761 cccgcgggcc gttcggaatt cggcggcgaa aactcggccc acagcgtctg atcgaccttg
     2821 tcgaacgatt cggcctgcac aagctggctc accttgttcg tcagcccttg ccgcgtctct
     2881 tccagcagcc gcacgttgtt gatatagacc ggctcggcgg tgcggcgggc ctccgccttg
     2941 cgtttttcgg tttcggcgac gttgggcagc tcgaagttga cgcgagcgac aattttccgc
     3001 tgcggcacgt ctccgacgcg gaaggcaaac ggcgggatgc cggccccagt cacggcccac
     3061 aggatcatgc aagccgcaat ccacaacccc aaccgcagca gcacgtccgg gcggcgcgcg
     3121 gtcgcccaga gctgcgaaaa gaaattcggc ggcagcgcga gggaaccgcc tcggtagggg
     3181 cgctgccggc ggggggatgc gttggacatg gggggaaatt cagaatgcag aatttagaat
     3241 gcagaaatcg gaattccaat cattctgcat tctgcattct gaattccgca tttgattacc
     3301 ggcctttcct catcgctgcc ttctggccct cttcctcgta cgcccggacg atgtcctgca
     3361 ccagccggtg ccggacgata tccgacttgt taagctgcac cacgcttacg ccttcaatat
     3421 cccgcaggcg gcccagggcg tcgatcagcc cgctggcgct gggcctgggc aggtcgatct
     3481 gggtcgtgtc gcccgagacg acgattttcg agccctggcc catgcgagtc aggaacattt
     3541 tcatctgggc gatcgtcgtg ttctgggctt cgtcgagaat gatgaacgcc tcgttgagtg
     3601 tccggccgcg catgtacgcc agcggcacca cctcgatcac ttcctgctcc atgtactttt
     3661 tcatctggtc gaagtcgatc atctcgtgca gggcatcgag cagcggccgc aggtaggggt
     3721 tgatcttcgc ccgcaaatcg cccggcagaa agcccaggct ctcgcccgct tcgacggcgg
     3781 ggcggaccag cacgatcttc cgcatctgat ggtgcttgag ggcctccacc gccagggcga
     3841 cggccaggta cgtcttgccc gttccggcag ggccgacggc aaacgtgatg tcgtgcttgc
     3901 ggatcgaatc gatgtaccgc gcctgcccct gcgtgcgggg ggcgatgcgg cgggcggctt
     3961 gaatgacatc gaccgactgc tggagggcca ctgcccgctt gccggtgacg tgggccaggg
     4021 cgtcggccac gtggtccagg ccgatctcct tgccttcgtc gagctgcgta ctgagctgcg
     4081 tcagagccgc cgtcgcccgg gccaccgccc cttcttcgcc ggccacgcgg atctcgccgt
     4141 cgcggtgcgt gatcgagacg cccagggcgt cgcgaatcgt ccgcacgtgc tgatcgcggg
     4201 cgccgaacag ggtgagcact cgatgagaat cgaccagcgg aattgtcgct tcggtcatgc
     4261 gccgacgaca gtcgccagac tgccgcacct ggattcattt cgccccagcc cagccggcca
     4321 gaagctgcaa cattgtaata tactcctgcc cgatgcgagg gcggtaggcg agcacggctg
     4381 gctgatatgt aacccctgtc tgcgaaaggc tttcggatcg gcgttgtgca tgtcggcggg
     4441 gcgcgtaacg ggcgctggga atacttgggg gaattcggga ataaatgaac ttccgtgcgc
     4501 atctataagg aagggagtgg ctgcgcgcgg atggttgctg acgccgggaa tccggtgcga
     4561 ccaaccgcgg cagaatgggc ggggtcgatg acttgcaagt tgcgctcgcc gctcaggaaa
     4621 atggaggaaa aatggttgac aacgcgcaac tgcgcaactt cctcaatcgg gggatttgcg
     4681 cttcgcaagt aattggtgcg atgggagtta ggatgatgtt acgcgacgcg gcagggcgag
     4741 aaaaaagccg gaaagttgcg cgccgcgcgg gctgaatgga gcgttgccgg gacgatgaga
     4801 ctgacgtaca aaatccccat gccagcgaaa cggagttcca ctagcgctgc cgcgatcgac
     4861 ggtggtccag cgttaaatca gatgctgcag ggcgatgccc tggaggtcct gcggacgtgg
     4921 ccggaggggc tggtcgattg tgtggtgacc agcccgccct attggcggca gcgggaatat
     4981 gctgccagcc gtgagcaggt gggccaggag gcttcgcccg cggcgtatgt cgagcgcttg
     5041 acggagatct ttcgcgaatg ccggcgggtg ctgaaggcgg cgggctcgct ctggttggtg
     5101 atcggcgaca agtacgacga cgggcggcag ctcggcttgc cgtggcgggt ggccctatcg
     5161 ctgatcgatg atggctggat cctgcgagcc gactgcatct ggcacaagcc gaacgcgatg
     5221 cccagctcga cgaaatcgcg gccgacgacc gaccacgagt acattttttt cttcacccaa
     5281 tcgcccgact attactacga cgccgacgcc atccgcgagc cgcacgtgac cttcagcgac
     5341 aagagccgca tgcggggcgg acgggggcac tttggccggc gcggcggcac gcccgaagcg
     5401 gggaagaacg gcggcagcca gaacctgcac gacggccgct gggaccaggc gtttcatccg
     5461 ctgggtcgga acaagcggac cgtctggtcg atcccgctct cgaagtttcg cgaggcccat
     5521 tttgccgttt tccccgaatc gctggtcgag acgtgcatcc gcgcgggctg ccctgccggc
     5581 ggcgtcgtcc tcgatccgtt cctcggcagc ggcacgacgg ccgtcgtcgc ccagcgcctc
     5641 ggccgatcct atctgggaat cgactgctcc gcggaatact gtgaaatggc ccgccgccgg
     5701 atcggcaatg gccagccgtt gtttgactag cgggtgttcc caacaggcgc gactcgtagc
     5761 cgcgttcgcc agaacgcggg aacgccgacc atatcgcccg cactctggcg agtgcggcta
     5821 ccgcttgtga ttagccgcag ccatgattcg ccgcctccac tatctcgccg cagtggcatt
     5881 gatcgctctg gctgcggctg cgggcgctca agagctgccg ccgggaatcg acgagtacgt
     5941 cgaagccgct cgaagcaagt ggcaggtgcc ggcgctgtcg atcgcggtcg tgaaggatgg
     6001 caaggtggtg caccttcgcg gctacggccc gcggtcgatc ggcggcagcg agccggtcga
     6061 tggccagacg gtattccgct tggcgtcgat ctccaagaca ttcactgccg cgaccgccgc
     6121 gctcttggtc gaagagggca agctcacctg ggacgatccg atccggaagc acgtgcccga
     6181 gttcgaattg cacgatcgct atctgaccga gcacgtctcg cttcgcgatt gcctgagcca
     6241 ccgcgtcggc ctggagaccg gcgacatcgt cgcccgccgg ggcgatctgt cgcgcgacga
     6301 aatcctcgcc cggctcagat tcctgcagcc gtattcgccg tttcgcggca agttcaagta
     6361 caacaacctg atgtatgtcg cggcaggcga aacggtcgcc cgtgcggcgg gccagtcgtg
     6421 ggaggacttc ctttcacggc ggctgctcgt gccgctgggc atgcggtcga ccacgcccac
     6481 cttcgcgagt ctgcacgcgg acaacctggc caccgcctat cgcctgcacg acggcgaatt
     6541 gcaggccgtg acgaccgcgc cgatcatcga cgccgtcgcg ccggccggct cggtccactc
     6601 gtcagcagcc gacatggccc gctggctgca gttctggctg gcagaagggg aattggacgg
     6661 ccagcgactc ttgcagcgcg gcactgtgcg ggaaatgctg gccatgcaca gcacggtgcc
     6721 catcgagcgg agcgacgaca gcaacatcta ccgggcgaag ttctatggct ggggcctggg
     6781 ctggtcggtg ctcgattatc gcggccgcaa gatccacacg cacgccggcg gcagcggcac
     6841 gttcatcggc ttcatgcccg aagcggggat aggcgtcgtc gtgctgacga acctggagtt
     6901 cacgaatctc ggcgggatgc tgatgtacga cgtgttcgac gcgtatctgc tcgatcgcga
     6961 gcgggcctgg aaccgcgacc aatggccgct gtggctcgcc gtcgatgaac cgccggaaat
     7021 caccggcgac aaggcccgcg ccaagctcga cgcaatgcga aaggccggca ccagccctac
     7081 gctctcgctg gcgcagctcg cgggcaagta tcgcagtgat ttatacggcg agatcgagat
     7141 tctcgacggg gacggcggcc tgcagcttaa actgggacgc aatccgccgg tcaagctcca
     7201 gcattggcag gacgacacgt tcgtctcgcc gggcccggaa gccgacgctc cctggttcga
     7261 ctggctgctc aagttccgca tcaccgccgg cgagtgcgag gcgctcgaca tcgagcgcat
     7321 cggctgggac gagccgctgc caacgttcca ccgcgccgcg gattcgtaac atgtcgttcg
     7381 acacgcgttg atagtaaccc gaagcgtcag cgagggagca tgagactcac acattatcgc
     7441 tctgtagcct cgattgctgc atggttcgcc gccgtcggcg ccgttcgtgc cgccgagcct
     7501 tccacgattc cgggcaaacg gctgccggag atcaaagagc cggtgctcct tggcacggcg
     7561 gaagccgatg ccattgcggc ggctctgcag gtgtttccgc ccgacaacgc ctggaatacg
     7621 gatatcagcc gctggccgct gcacccgaac tcggcggcga tcgtcgcctc gatcggcaac
     7681 gacaagccgc tgcgctacaa caccgacatg agctacgtct ttgtgccgcc cgggcagaag
     7741 cttgtcgatg tggcgatcgt ggaatacccg gacgaatcgg acaaagggcc gtacccggtg
     7801 ccggataaca caccgatcga gggctggccc gcctggcatc gccgcgatgc cgagcagaag
     7861 ctgacgctcg aagaggtgca gcgcgacaag ctcaaccaag gcggcgaccg gcacgcgatc
     7921 gtcgtcgatc cgtacgccgg aaagctctac gagttctatc agatgaagcg gaccgacccg
     7981 gcgagcgggg aacaggcgag cggggggcgt cggctccctg agcctcgctg gcagtgcgcc
     8041 tgtgccgcca tcttcaatct gcgtacgaat gacctccgcc agggcggctg gacgagtgcc
     8101 gatgccgccg gcctgccgat ctatccagcc gtcatccgct acgacgaatt ggcggccggc
     8161 aaggtcaacc atgcgatgcg ggtgacggtc cgcagtacgc ggcgggcgta tgtcgccccc
     8221 gccacgcact tcgccagccg caagaccgac gagaacctgc cgcggatggg cgagcggctg
     8281 cggctgaagg ccggttacga catccgcggg ttttcgcccg aggtgcagac gatcctgaag
     8341 gggctgaagc aatacggcat gctcgtcgcc gacaacggcc tcgagtgggc gatcagttgc
     8401 gcccccgacg agcggatccc cgtcctccac gaagagctgc gccgcgtgaa gggctcggcg
     8461 tttgaggtgg tgcgggcgcc ggagcgctag gtctttgagc cgcagcataa cctacggcgg
     8521 tcccccttcg cagcaggtct cgataatggt gtggcaccgc tcgcactgca gcttgccacg
     8581 aatctcgagc agatgcccgc cgcaaacggg gcaggcgtaa cgcggagcag gctttgcgcc
     8641 gctatcacgc ttggagtcgt ggctggtccg ctggtcgtcc cggtcgttca tcgtcgccct
     8701 gcaatctgcc cgacgctgac ctcagcgtag ggatactcgt ccagcaacgc ggcatacagc
     8761 ggcaccgtgt aaagcagatt ggcaagttgc agaggggaga acttgcgtac gtctgcctgg
     8821 caatgcgctg cgattcgcaa cggcacggtg accgtcaatt cccggccttg cgcttccccg
     8881 gattcgcggg tggcgatgat atagccgaag atggaaagtg gcagcgacca gagaccgatg
     8941 acaaacagcc accaccaact gtacccggtc gagtacggct tctcgcacac aaccgtgaac
     9001 atcatcacgt cgctcgtaac gattccactc agggcgcatt cgcgtcctgg cgcctcgcac
     9061 cggctctgca acatcgatcg aatgcggtcg atcgcgctga cttggaatgc agcctgcccc
     9121 gcctgccgcc gcagttcgct cagccgcgga accttgacga cgcgcccgca cgggcaaggc
     9181 atgctgccgc cagccgccgc tgtggatacg ggaagcgcgg caccgcacgc acattggacg
     9241 gtatatcccg ttgcgacagg catgcaggca gaatacaccg cggcctacgg tcccggcaaa
     9301 tacggcttcc aatacggcga gcggtcgttt agatcgtact gcggatagtg cccgtcgagc
     9361 cagggctggg caaagttgac cggtggctgt ccgcggaagc cgccaccgcc gccggggaag
     9421 ccgggaccga tcggcccgaa tccgccgcca aatcggccac caaagccgcc gccgctgtac
     9481 ggatagaagc cgttgccacc gcccaggccg ccaaagggcg ggccccaggc gtcgtacggc
     9541 accgagaacg gccggtacgg gaaccgccat tcttcgtagg gccgcaccgg ccgacc
//
LOCUS       DASUGB010000015         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_974656, whole genome shotgun sequence.
ACCESSION   DASUGB010000015 DASUGB010000000
VERSION     DASUGB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974656"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFV87_00275"
     CDS             <1..976
                     /locus_tag="VFV87_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5102213.1"
                     /translation="QMGETPGDWGFDEYITDPTASGWFWKTSYTKNGKEMSTDQETYY
                     PDIASDFAIDFMRRHKEGPFYFYLSEHLIHGPILKTPDSKPGAEPQQLYDDNIAYLDK
                     TVGKLLKALDELGLRQKTLVLFTTDNGTAPPAYMPQHDPDLSTGKIGGRAMNGRKGTL
                     LEGGSRVPLIASWKGTLKPGGIRADLIDFSDFLPTFAELAGAKLPADVKFDGTSFAPQ
                     LRGEEGKPREWIFVQLGAGWYARNDAFKLNQNGELFSMKDAPFVEAPIPADNADPAAT
                     AARKQLQAVLDDLNPAGGKTVAPGDAGKKKVGKKAKQKAKKAADSKAP"
     gene            complement(978..2402)
                     /locus_tag="VFV87_00280"
     CDS             complement(978..2402)
                     /locus_tag="VFV87_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102214.1"
                     /translation="MQPIPSSVARRTFLSRTACGIGGAALSSLFKQGISRAGDSSPLA
                     GRGVVNPLHFAPKAKRVIFLTMAGGPSHLETLDHKPKLAEMHGQPMPESFTKGQPIAQ
                     LQGAQLKCFAPQHPFKKFGASGQEISEIFPQIGAIADEICIIRSLRTEAINHDPAHTF
                     MNTGTTISGRPSMGSWLTYGLGAETENLPGFVVLGSSGRFGQSQPIASRQWSAGFLPS
                     RFQGVELRGKGEPVLYVSRPAGVSASQQADVVDAVRQLNKLEEEAIADPEIATRIAQY
                     ELAFKMQASVPELVDLSDEPKQVLDLYGTPGADGSFAANCLLARRLAERGVRFIQLYH
                     RDWDHHGGIKDQIAGIAKEVDQACAALITDLKNRGMLQDTLIVWTGEFGRTPMAQGNG
                     RDHHMKAFSAWLCGGGIKGGITHGATDELGYNAVENIVEVHDLHATLLFLLGIEHDRF
                     TYRWQGRDFRLTDVKGEVVKAVLA"
     gene            complement(2416..>2770)
                     /locus_tag="VFV87_00285"
     CDS             complement(2416..>2770)
                     /locus_tag="VFV87_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102215.1"
                     /translation="SNTPQQALVLLNDPTYVEAARSLAGRILALGGDTTDSRLDFAFQ
                     QVLTRSPRPEEVKLLTALLEKHRQEFAADKPAAEQFLKVGDLVSASSLDPAELAAWTS
                     VSRVLLNLHETITRN"
BASE COUNT          519 a          889 c          873 g          489 t
ORIGIN      
        1 gcaaatgggc gagactcccg gcgactgggg ctttgacgag tacattaccg atccaaccgc
       61 aagcggctgg ttttggaaga cgagctatac caagaacggc aaagagatgt cgaccgacca
      121 ggagacttat tatcccgaca tcgcctccga tttcgccatc gacttcatgc gccgccacaa
      181 agaaggccca ttctatttct acctgtcgga gcatttgatt catgggccga tcctgaagac
      241 gcccgacagc aagcccggcg ccgaaccgca acagctttac gacgacaaca ttgcgtatct
      301 cgataagacc gtcggcaagc tgctgaaggc gctcgacgag ctgggccttc gccagaagac
      361 gcttgtcctc ttcacgaccg acaacggcac cgccccgccc gcttacatgc cgcagcacga
      421 tccggatctg tccaccggca agatcggcgg ccgggcgatg aacggccgca aagggacgct
      481 gctcgagggc ggctcgcgcg tgccgctgat cgcaagctgg aaaggtacgc tcaagccggg
      541 cggcatccgc gccgacctga tcgacttcag cgattttctc cccaccttcg ccgaactggc
      601 cggcgccaag ctgcccgccg atgtaaagtt cgacggcacg agctttgcgc cgcagctccg
      661 cggcgaagag ggcaaacccc gcgaatggat cttcgtccag ctcggcgccg gctggtacgc
      721 ccgcaacgac gccttcaagc tcaaccaaaa cggcgagctc ttttcgatga aagacgcccc
      781 cttcgtcgaa gcccccattc ccgccgacaa cgccgacccc gccgccaccg ctgcacgcaa
      841 acagctccaa gccgtcctcg acgacctcaa tccagccggc ggcaaaaccg tcgcacccgg
      901 ggacgctggc aagaaaaagg tcggaaaaaa ggccaagcag aaagctaaaa aggccgccga
      961 ttccaaggcg ccctaggtca ggccagcacc gcctttacca cctcgccctt gacgtcggtc
     1021 agcctaaagt cgcggccttg ccagcggtag gtgaagcggt cgtgctcgat gccgaggagg
     1081 aagagcagcg tggcgtggag gtcgtggact tcgacgatgt tctccacggc gttgtagccc
     1141 agctcgtcgg tcgcgccgtg ggtgatgccc cccttgatgc ctccgccgca gagccaggcg
     1201 gagaaggcct tcatgtgatg gtcgcggccg ttcccctggg ccatgggcgt gcggccgaat
     1261 tcgcccgtcc agacaatgag cgtgtcctgc agcatgccgc ggttcttgag gtcggtaatc
     1321 aatgcggcgc aggcctgatc gacttccttc gcgatgccgg cgatctggtc cttgatgccg
     1381 ccgtgatggt cccagtcgcg gtgatagagc tgaatgaacc gcacgccacg ctcggccagc
     1441 cggcgggcta agaggcaatt ggccgcgaag ctgccgtcgg cgccgggcgt accatacagg
     1501 tccagcactt gtttcggctc gtcggacaga tcgaccagct cggggacgct ggcctgcatt
     1561 ttgaacgcca gctcgtactg ggcgatgcgc gtggcgattt ccggatcggc gatcgcttct
     1621 tcttcgagct tgttgagctg ccggacggca tcgaccacgt cggcttgctg gctggcggaa
     1681 acaccggcgg gccggctgac atagagcacc ggctcgccct tgccgcggag ctcgaccccc
     1741 tggaagcggc tcggcagaaa gcccgcactc cactggcggc tggcgatcgg ctgcgactgg
     1801 ccaaatcttc cgctggaacc cagcacgacg aaccccggca ggttctcggt ctcggcgccg
     1861 aggccatacg tcagccacga gcccatgctg ggccggccgc tgatcgtcgt cccggtgttc
     1921 atgaaggtat gagccgggtc gtggttgatc gcctccgtcc gcagcgagcg aatgatgcag
     1981 atctcgtcgg cgatcgcgcc gatttgcgga aaaatctcgc tgatctcctg ccccgaggcg
     2041 ccgaacttct tgaacggatg ctgcggggca aagcacttga gctgggcgcc ttggagctgc
     2101 gcgatcggct ggcccttggt gaacgattcg ggcatcggct ggccgtgcat ttccgccagc
     2161 ttgggcttgt ggtcaagggt ttccaggtgc gacggtccgc cggccatcgt cagaaagatg
     2221 acccgcttcg cctttggcgc aaaatgcagc ggattgacca caccgcggcc cgccagcggc
     2281 gacgaatcgc ccgcccggct gattccctgc ttgaaaagag acgacagcgc cgcccccccg
     2341 atgccgcagg cggtgcggct aaggaacgtg cggcgggcga cagaggatgg aattggttgc
     2401 atgttcggtt cgtgtttagt tacgtgtgat tgtttcgtgc aagttcagca gcacgcggct
     2461 gacgctggtc caagcggcga gttcggcggg gtcgagcgac gacgcagaaa cgaggtcacc
     2521 gactttgagg aactgctcag ccgcgggctt atctgcggcg aattcttgcc ggtgcttttc
     2581 gagcagggcg gtcagcagtt tgacttcttc cggccgggga gaacgcgtca atacttgctg
     2641 gaaggcaaaa tccagccgtg aatcggtcgt gtcgcccccg agcgcgagaa tccgccccgc
     2701 cagcgagcgg gcagcttcga cgtaggtcgg gtcattgagc agcaccagcg cctgctgggg
     2761 cgtgttgctc
//
LOCUS       DASUGB010000016        10433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1947247, whole genome shotgun sequence.
ACCESSION   DASUGB010000016 DASUGB010000000
VERSION     DASUGB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947247"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFV87_00290"
     CDS             <1..1104
                     /locus_tag="VFV87_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5102216.1"
                     /translation="LALSSILVNPQFLFRIERDPAGVEPGAVYPIDDFALASRLSFFL
                     WSSLPDDELLDLAADGKLREAEIFDAQVRRMLADPRAASLVSNFAAQWLYLRNLEAAQ
                     PDMRLFPDFDDNLRQAMRRETELCFERIVREDRSVLDLVRADATYLNERLARHYGIPH
                     VYGSRFRRVALDDDRHRGGLLRQGSILTVTSYATRTSPVIRGKWVLENLLGSPPPPPP
                     ANVPDLKDNTVSSRLSVRERLAEHRANATCANCHNLIDPAGLSLENFDAVGRWRTLEE
                     GRPINASGGLPDGSSFAGVAGLERALLDRPELFVQTLTEKLLTFALGRGIEHYDAPAV
                     REIVRDAKDNEYRFSQIILGIAHSTPFQMRKAP"
     gene            1101..2447
                     /locus_tag="VFV87_00295"
     CDS             1101..2447
                     /locus_tag="VFV87_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5102217.1"
                     /translation="MIVKKCALPRRTFLRGAGAALALPLLDAMVPALTAQDQSPAAPA
                     RLRRLGFVYMPMGCDVTRWTPPGESLDELSPTLSPLGPVKEKIAVISNLELRNAYPGT
                     HATSNASFLSAAKAKRTESTDYYLGTTVDQIAARQIGAGTQLPSLELSMDLMSVVGQC
                     DNGYACVYQNNLSWSSPTTPLPAEAHPRIVFENLFGEEGRPEQRRAALKKRASLLDAV
                     TEELQRLQKSLGPADRRRVDESLQTVRDVERRIEQAEAAAKDDKQPDLERPLGVPASY
                     ADHARLMFDLQLLALQGDITRVVTFQLARETSNRTYAEIGVSEPHHPLTHHGNDPAKI
                     AKVAQINRFHVSLFAEFLQKLAATTEGDGSLLDHVLYLYGSGMGNPNVHDHVNLPIIV
                     AGGAAGRMRGGRHIQYREPTPLANLHLTLLDKVGVRLDSFADSDGKMDELFQPLAM"
     gene            2485..3957
                     /locus_tag="VFV87_00300"
     CDS             2485..3957
                     /locus_tag="VFV87_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5102218.1"
                     /translation="MKLLEGRRMVPGVFLMLALACASAVAGESRALVANAAEQQDRSK
                     IRAVLDARADVKAPQADGMTALHWAAYHDDAEVVRLLLAAGADAKAANRYGVTPLSLA
                     CINGNGDIARQLLAAGADPNAALPGGETPLMTAARTGRLGAVQALLNHGAKFNAKERK
                     GQTALMWAAAEGNVEVVDALLKAGADFRTPLRSGFTPFFFAIREGRDNVVARLLEAGI
                     DVNEPLRPATGKAMTPLLLAVENGHFELAAALLQAGADPNARPAGYTALHAINWVRKP
                     IRGDGDPPPIGSGQLTSLDLVRELVAHKADINCRLEKGESGRGRFTTTGSTPFLVAAR
                     ASDVPLMQLLLELKADPAIANADDSPPLLAAAGVGALNDGDESAGTEEEALAAVRLLL
                     QLGADVNAVDKNGETAMHGAAYQSRAKVVELLAEHEADVKIWNRKNKFGWTPLMIAQG
                     HRPGNFRPAPDTIAAIERIMRSAGDGPPRPAPRGASPRND"
     gene            4103..5578
                     /locus_tag="VFV87_00305"
     CDS             4103..5578
                     /locus_tag="VFV87_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5102219.1"
                     /translation="MTLARRFAVLGCVGAALFAIALPPSRAAAEERLPNFVVIYCDDL
                     AYTDVGPFFDAPGDAADSKPTENRSKKGYSTPNLDRMAAEGMKLVDFHSAAAVCSASR
                     VALLTGCYPQRVGILGALGPANKHGIHDDEILLPEILKKQGYATAIFGKWHLGHHPQF
                     LPTRHGFDRYFGLPYSNDMWPNHPTNKSFPPLPLIEGEKILEHNPDQTQLTTWYTERA
                     VQFIADSKDQPFFLYVPHSMPHVPLFVSDKFKGKTERGLFGDVIAEIDWSAGQILEAL
                     KKNGVDEQTLVLFSSDNGPWLSYGDHAGSAGPLREGKGTTWDGGHREPCVARWPGKIP
                     AGSVCTELAATIDVLPTFAKLAGSEAPQDRIIDGRDIWPLLSGQPGAKSPHEAFYYYW
                     DNGLEAIRSGPWKLHLPHAYRTLAGQPGHGGQPGPYKQARTEQALFKLTEDVGETTDV
                     SAEHRDVVQRLLAYAEQAKEDLGDSLTKREGKNRRPAGQLK"
     gene            5617..6036
                     /locus_tag="VFV87_00310"
     CDS             5617..6036
                     /locus_tag="VFV87_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102220.1"
                     /translation="MEPTSLAKPPRRFRFQFGLKWLLLAMLLVAVYLAGRSSMTYRNV
                     FAPKIEGNWTATMPRGHQRTVTVQNVGDGRYTLATGGVLSGTYEWQNGKLVVVQPGDS
                     RMVGLVWTWDGEKLILTGEPANTPTGSSYTGATLTRP"
     gene            6163..6651
                     /locus_tag="VFV87_00315"
     CDS             6163..6651
                     /locus_tag="VFV87_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX5102221.1"
                     /translation="MKIGLFADTHDHLANLRLAVEHFNRAKCTLVLFAGDLVSTFAVP
                     PLRKLKCPLVGCFGDNEGNKVGLLAGFSLVEGQIGEPPVRYTSADGTRFVLCHMERQL
                     RGLDDAWDIALFGHTHKPRVRQGAGGRLLINPGETSGWTFGQPTIGFLETTTREVEIV
                     RL"
     gene            6672..8525
                     /locus_tag="VFV87_00320"
     CDS             6672..8525
                     /locus_tag="VFV87_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5102222.1"
                     /translation="MTSATQAQSNQSKDKPIRVAILGGGVGGMTAAHELVERGFEVAV
                     YEMRADPGGKARSIPVPDGLTNGKKPLPGEHGFRFFPGFYKHIPDTMKRIPSGKKRVY
                     DNLVPTKHTLIARAQGMADLEFPAQPPASPWQMLRHFKPLFQTDLGIPDRDLRFFASR
                     LLMLLISCDQRRLKDYERRSWSEFAEADERRSPAYRKYCADGITRALVACQAAKMSAR
                     TGGYILLQLMFDLVQPGVQANRVLNGPTSEVWFQPWLEYLKKRGGNKFQFHPSSKVTQ
                     FHFAEGLITGVTIQENGAPQTITADYYISALPVEVLEPRAGGPSAGDPQPLITEEMKA
                     FDPSLAHLHKLETEWMNGIQYYLDKDVPVVAGHTMFIDSPWSLTSISQKQFWPDVDLT
                     QYGDGNVKGVLSVDISNWDEKGSLGKPARECTPTEIKDEVWKQIEDALNDDEKKELNN
                     GAKVLHWFLDTAIHFPNPNTMGNAEPLLINTAGSWEHRPEAVTRIKNFFLAADFVRTH
                     TDLATMEAANEAARRAVNGILKASGSRKRRCKVWPLDEPWLFAPLKYYDRLRFRRLPD
                     RPPSRTLIWLVLCLAVPIWLAIHLAWRLYFAIVEGLIAPVWQWLLRTFWRS"
     gene            complement(8586..>10433)
                     /locus_tag="VFV87_00325"
     CDS             complement(8586..>10433)
                     /locus_tag="VFV87_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102223.1"
                     /translation="AAALAPSERMALKTLRADYLVRSTGPGDHDEMEVYHDRVRETLV
                     EHLSAAELQDCHRRLAASLEASAHADPEALASHLQAAGEPARAGHYYAVAADRAAAAL
                     AFDRAARLYRLALDLEPNDAPDRRGLRTRLGDALANAGRGAGAAREYLAAADGAPAAE
                     SLELRRRAAMQSLISGHIDDGLQALGTVLDSVGLRLAGTPRQALWSLLWRRTQLWLRG
                     LGFKERDSSQISAEALTRIDICWSAAVGLSIVDTIRGADFQSRNLLLALGSGEPYRIA
                     RALAWEAAHTSTSGLPARRTTARLLEAAGRLADRIEHPHALGMVMLCRGISGYMEGRW
                     KDGRQFSEQAEAVFRDHCTGVAWELDTAHSFANWSLFFLGEVAELTRRLPILLQEARE
                     RGDLYSATNLGTFVGHLTWLAADDPEGAEHDMHQVMALWSHRGFHVQHLTGLMGRIQI
                     ALYQGDGAKAWQIVTSEWPDLERSLFLRVQTVRLFMRDLRARSALAAAREAADPRPYL
                     RSAGRDARRIEQEDASWSQPLAERIRAALAAAGGQEPEAAQFLERATAGFDAANMHLF
                     AAAARRRRGQLLGGDQGRALIDQADLWMQQQKISNGRRMTGLYMPGFAD"
BASE COUNT         1861 a         3371 c         3478 g         1723 t
ORIGIN      
        1 ctggccctca gctccatcct ggtcaatccg cagtttttgt tccggatcga gcgcgatcca
       61 gcaggcgtgg agcccggcgc ggtttatccg atcgacgatt tcgctcttgc ctcgcgtctc
      121 tccttctttc tctggagcag cctgccggac gatgagctgc tcgacctggc ggccgacggc
      181 aagctgcgcg aggcggagat tttcgacgct caagtgcggc ggatgctcgc cgatccgcgg
      241 gccgcgtcgc tcgtcagcaa ctttgccgcc cagtggctct atctgcggaa cctcgaggcc
      301 gcccagcccg acatgcgcct gtttcccgat ttcgacgaca acctgcggca ggcgatgcgc
      361 cgcgaaacgg agctgtgctt cgagagaatc gtgcgcgagg accgcagcgt ccttgatctg
      421 gtgcgggccg acgcaaccta tctgaacgag cgcctggcca ggcactatgg cattccgcac
      481 gtgtacggca gccggtttcg gcgggttgct ttggatgacg accgccaccg cggcgggttg
      541 ctccgtcagg ggagcattct gacggtgact tcgtacgcga cccgcacgtc gccggtcatt
      601 cgcggcaagt gggtcctgga aaacctgctt ggctcacccc ctccgccgcc ccccgccaat
      661 gtgccggacc tgaaggacaa cacggtctcg tccaggctct cggtccgcga gcggctggcg
      721 gagcaccggg ccaatgccac gtgcgcgaat tgccacaatc tgatcgatcc ggccggcctg
      781 tcgctggaga acttcgacgc cgtcggtcgc tggcgaaccc tcgaagaggg ccggccgatc
      841 aatgcttccg gcggcctgcc ggacggcagc agcttcgccg gcgtcgcggg cctggagcgg
      901 gccttgctcg atcggcccga actcttcgtg caaacgctga ccgagaaact cctgactttc
      961 gccttgggtc gcggcatcga gcactatgac gcacccgccg tgcgagaaat cgtccgtgac
     1021 gcgaaagaca acgagtatcg cttttcgcag atcatcttgg gcatcgcaca cagcaccccg
     1081 tttcagatga ggaaggcgcc atgatcgtca agaaatgtgc cctgccacgg cggacgtttc
     1141 tgcggggcgc aggcgctgcg ctcgccctgc cgctcttgga cgcgatggtg ccggcgctga
     1201 ccgcgcagga ccaatcgccg gccgctccgg cgcgattgcg gcggctgggc ttcgtttata
     1261 tgccgatggg ctgcgatgtg acgcgctgga cgccgcccgg cgagtcgctc gacgagcttt
     1321 cgcccacgct cagcccgctt gggccggtca aagagaagat cgcggtgatt tcgaacctgg
     1381 agctgcgcaa tgcctatccc ggcacgcatg ccacatcgaa cgcctcgttc ctgagcgcgg
     1441 cgaaggccaa gcggaccgag agcaccgact attacctggg cacgacggtc gatcagatcg
     1501 ccgcccggca gatcggcgcg ggcacgcagc tcccctcgct ggagctgtcg atggacctga
     1561 tgtcggttgt cggccagtgc gacaacggct atgcctgcgt ttatcagaac aatctttcct
     1621 ggtcgagccc cacgacgccg ctgccggcgg aggctcatcc gcggattgtc ttcgagaact
     1681 tgttcggcga ggaagggagg cccgaacagc gccgcgcggc cctgaaaaag agggctagcc
     1741 tgctcgatgc ggtgaccgaa gagctccagc ggctgcagaa gtcgcttgga ccggccgatc
     1801 gccgccgtgt cgatgaatct ctccagaccg tccgcgacgt ggagcgccgc atcgagcagg
     1861 ccgaagccgc cgccaaggac gacaagcaac cggatctgga gcggccgctg ggtgtgcctg
     1921 cgtcgtatgc agatcatgcc cgcctgatgt tcgatctgca gctcctggcg ctgcaaggcg
     1981 atatcacgcg agtcgtcacg tttcagctcg cccgcgagac cagcaaccgc acgtatgccg
     2041 agatcggcgt ctccgagccg caccatccgc tcacgcacca cggcaacgat ccagcgaaga
     2101 tcgcgaaagt tgcccagatc aatcggttcc acgtctcctt gttcgccgag ttcctgcaga
     2161 agctggccgc gacgacggaa ggggatggct cgctgctcga tcacgtcctc tatctctacg
     2221 gcagcgggat gggaaacccg aacgtccacg accacgtgaa tctgccgatc atcgtcgccg
     2281 gcggtgcggc cgggcggatg cgcggcggcc ggcacatcca gtaccgcgag ccgacgccgt
     2341 tggccaatct gcacctgacg ctgctcgaca aagtcggcgt ccggctcgac tcgttcgccg
     2401 acagcgacgg caaaatggat gagctattcc agccgctggc gatgtaggag tgagtggtga
     2461 gtggtgagtg gtgagtgtgg cgaaatgaaa ctgttggaag gacgacgaat ggtgccgggc
     2521 gtgttcctga tgttggcttt ggcatgcgca tccgcagtcg ccggcgaatc ccgagcgctg
     2581 gttgcgaatg cggccgagca gcaggaccgc tcgaagattc gagccgtgct cgacgcgcga
     2641 gccgatgtca aggctccgca agccgacggc atgacggcgc tccattgggc ggcgtatcac
     2701 gacgatgcgg aggtggtgag gctgctcctc gctgccgggg ccgatgcgaa agcagcgaac
     2761 cgctacggcg tcacgccgct gtcgttagcc tgcatcaacg gcaacggcga catcgcccgg
     2821 cagttgctgg ctgctggtgc ggatccaaat gccgcgctgc ccggcggcga gacgcctttg
     2881 atgaccgccg cccgcacggg ccggctcggt gcggttcaag cgctgctgaa ccatggggcc
     2941 aagttcaacg ccaaggagcg caaggggcaa acggccctca tgtgggccgc agccgaagga
     3001 aatgtcgagg ttgtagacgc cctgctgaag gccggcgccg atttccgcac gccgctgcgc
     3061 tcgggcttca cgccgttctt tttcgccatc cgcgaagggc gggacaatgt tgtcgcccgg
     3121 ctgctggagg cgggcatcga cgtaaatgag ccgctgcggc cggcaacggg caaggcgatg
     3181 acgccgctcc tcctggccgt cgagaatggc catttcgagc tggccgcggc tttgctccag
     3241 gcgggggccg atcccaatgc ccggccggcc ggctacacgg cgctgcacgc catcaactgg
     3301 gtccgcaagc caatccgcgg agacggcgat ccgccgccga tcggttcggg ccagctcacg
     3361 agcctcgatc tggtccgaga gctggtcgcc cataaggccg atatcaactg ccgcctggag
     3421 aagggcgaat ccggccgcgg acggttcacg acgaccggct cgacgccttt cctggtggcg
     3481 gcccgggctt cggacgttcc gctgatgcag cttttgctcg aattgaaggc cgatccagca
     3541 attgctaacg ccgacgactc gccgccgctc ctggccgcgg ccggagtcgg cgcgctgaac
     3601 gacggcgacg aatcagccgg caccgaggag gaagcattgg cggctgtccg cttgctgctg
     3661 cagctcggcg cggacgtgaa cgccgtcgat aagaacggcg agacggcgat gcacggtgcg
     3721 gcctatcaaa gccgggccaa ggtcgtcgaa ctgctggccg agcacgaggc ggacgtcaaa
     3781 atctggaaca gaaagaacaa attcggctgg acgccgctga tgatcgccca gggccaccgc
     3841 cccggcaact tccgccctgc gccggatacc atcgccgcga tcgagcgcat catgcggtcc
     3901 gctggcgacg ggccgccccg gcccgcgccg cgcggcgctt ctccgcgcaa tgattgaatc
     3961 agggcgggcc tcgcgccggg gcggaactaa ttggcttcga atgcggcggg gtgggagctg
     4021 acgatcttca ccgtcccctg cggggcgtta caatccgcgg agggtgcata gagatcgccc
     4081 atcgaaacac gggagaatca tcatgacgct ggctcgccga ttcgctgtgc tgggttgcgt
     4141 tggggccgca ttgttcgcga ttgccctgcc tccgagtcgg gcggctgcgg aggagcggct
     4201 gccgaatttt gtcgtgattt attgtgatga cctggcctat acggacgtcg gtccgttttt
     4261 tgatgcaccg ggcgacgctg cggattcaaa gccgaccgag aaccgctcaa aaaagggcta
     4321 ctcgacgccg aacctggacc ggatggcggc ggaggggatg aagctggtcg attttcattc
     4381 ggcggcggcg gtgtgcagtg cgtcgcgcgt ggcgctcttg accggctgct atccgcagcg
     4441 ggtgggaatt ctcggggcgc tgggaccggc aaacaaacac ggcatccacg atgacgaaat
     4501 cctgctgccg gagattctga agaagcaggg atatgcaacg gcaatcttcg gcaagtggca
     4561 cctggggcat catccgcagt ttctgccgac gcggcacggc ttcgaccgct atttcggcct
     4621 cccctacagt aacgacatgt ggccgaacca tccgacgaac aaaagcttcc cgccgctgcc
     4681 gctgatcgag ggcgaaaaaa tcctggagca caacccagac cagacgcagc tcacgacgtg
     4741 gtacaccgag cgggccgtgc agttcatcgc cgacagcaaa gaccagccgt tcttcctcta
     4801 cgtgccgcac agcatgccgc acgtgccgct gttcgtgtcg gacaagttca aggggaagac
     4861 cgagcggggg ctgtttggcg acgtgatcgc ggagatcgac tggagcgcgg ggcagattct
     4921 tgaggccctg aagaagaatg gcgtcgatga gcagacgctg gtgctgttca gctccgacaa
     4981 cgggccgtgg ctctcgtatg gcgatcacgc cggctcggcg gggccgctcc gcgaaggaaa
     5041 gggaacgacg tgggacggcg ggcatcgcga gccgtgcgtc gcccgctggc cggggaagat
     5101 tccggcgggc tcggtctgca cggaactggc ggcgacaatc gacgtgctgc cgacgtttgc
     5161 caaactcgct ggcagcgagg cgccgcagga ccggatcatc gacggccgcg acatctggcc
     5221 gctactttcc ggccagccgg gagccaaatc gccccatgag gcgttttatt actactggga
     5281 caacggcttg gaagcgatcc gcagcgggcc ctggaagctg cacctgccgc acgcctatcg
     5341 cacgctggcc ggccagcccg gccacggcgg ccagccggga ccgtacaagc aagcgcggac
     5401 ggagcaggcc ctgttcaagc tgacggaaga cgtgggagag acgaccgacg tgtcggccga
     5461 gcatcgcgac gtggtccagc gcctcttggc ctatgccgag caagcgaagg aggatctggg
     5521 cgattcattg acaaagcgcg aagggaagaa ccggcggccg gcggggcagc tcaagtagtc
     5581 ctgccaggcc cttggcagga cagcggtggc gacaatatgg aaccaacctc acttgccaag
     5641 ccgccgcggc ggttccgctt tcagttcggc ctgaagtggc tgctgttggc gatgctgctg
     5701 gtggcggtgt acttggcggg gcggtcttcg atgacgtatc gcaacgtctt cgcgccgaag
     5761 atcgagggga attggaccgc cacgatgcca cgcggccatc agcggacagt gaccgtgcag
     5821 aatgtgggcg acggacggta cacgctggca accggcggcg tgctcagcgg cacgtacgag
     5881 tggcaaaacg gcaagctcgt cgtcgtgcag ccgggcgaca gccggatggt cggcctcgtc
     5941 tggacatggg acggcgagaa gctgatcctg acgggcgagc cggcgaatac gccgacgggg
     6001 tcgagctata ctggcgcgac gctgacgcgg ccttaaggca agtggggcag gaatgttggg
     6061 tgggaccagc gagcgaagcg agcgcaggcc caccatgcgg catatacgga tggtgggccc
     6121 gcggcctgac ggccttgtcg caccctacga gattatccgc ctatgaagat tggcctcttt
     6181 gccgacacgc acgatcacct agcgaacctc cgcctggcgg tggagcactt caatcgtgcg
     6241 aagtgtaccc tcgtgctgtt cgcgggcgat ctggtttcga cgttcgccgt gccgccgctg
     6301 cggaagctga agtgcccgct ggtcggctgc tttggagaca atgagggcaa caaggtcggc
     6361 ctcttggcgg gcttcagcct ggtggagggc cagatcggcg agccgccggt gcgctacacg
     6421 tctgccgacg gcacgcggtt cgtcctctgc cacatggagc ggcagctccg cggccttgat
     6481 gacgcctggg acatcgccct gttcggccac acgcacaagc cgcgcgttcg ccagggcgca
     6541 ggcggccggc tgctgatcaa ccccggcgaa accagcggct ggaccttcgg ccagccgacg
     6601 atcggattcc tcgaaacaac gacacgggag gtcgagatcg tgagactgtg agctagacga
     6661 gggtttccac catgacatcg gcaacacaag cccagtcgaa tcaatccaag gacaagccca
     6721 tccgcgtcgc catcctcggg ggcggcgtgg ggggcatgac ggccgctcat gagctggtcg
     6781 agcgcggctt cgaagtcgcg gtctatgaaa tgcgggcgga tccgggcggc aaagcgcgga
     6841 gcattcccgt gcccgatggt ttgacgaatg gcaagaagcc gctgcccggc gagcacggat
     6901 tccgcttctt ccccggcttc tacaagcaca ttcccgacac gatgaagcgg attccgtccg
     6961 ggaaaaagcg ggtttacgac aacctggtgc ccacgaagca cacgctcatc gcccgggccc
     7021 agggaatggc ggacctcgaa ttccccgccc agccgccagc ttcgccgtgg caaatgctgc
     7081 ggcacttcaa gccgctgttc caaaccgatc tgggcattcc cgatcgcgac ttgcggtttt
     7141 ttgcctcccg cttgctcatg ctgctgataa gctgcgacca acggcggctg aaggactacg
     7201 aacggcgctc gtggtcggag ttcgccgaag cggacgagcg gcgctcgccg gcgtacagga
     7261 agtactgcgc cgatggcatc acgcgggccc tggtcgcctg ccaggcggcg aagatgagcg
     7321 cccgcaccgg cggctatatc ctcttgcagc tcatgttcga cctggtgcag ccgggcgtgc
     7381 aggccaaccg cgtgctgaac gggccgacga gcgaagtctg gttccagccc tggctcgagt
     7441 acctgaaaaa gcgcggcggg aacaagttcc aatttcaccc ttcaagcaag gtcacccaat
     7501 tccatttcgc cgagggactg attaccggcg tgacgattca ggagaacggc gcaccccaga
     7561 ccatcacagc tgattactac atttcggcat tgcccgtgga agtgctcgag ccgcgcgccg
     7621 gcgggccgtc ggccggcgac ccgcagccct tgattaccga ggagatgaag gccttcgatc
     7681 ccagcctggc ccatctccac aagctggaga ccgaatggat gaacggcatt cagtactatc
     7741 tggataagga cgtgcccgta gtcgcgggcc acacgatgtt tatcgactcc ccctggtcgc
     7801 tgacgtcgat ctcgcagaag cagttctggc ccgatgtgga cctgacgcag tacggcgacg
     7861 ggaacgtcaa gggcgtgctg tcggtggaca tctccaactg ggacgaaaaa gggagtctgg
     7921 gcaagcccgc gcgggagtgc acccccacgg agatcaagga cgaggtctgg aagcagattg
     7981 aagacgcgtt gaacgacgac gagaaaaagg aactcaataa cggcgcgaag gtcttgcact
     8041 ggtttctcga cacggcaatc catttcccga acccgaacac aatgggaaac gccgagccgc
     8101 tcttgatcaa tacggccggt tcctgggagc accgcccgga agcggtcacc cgcatcaaga
     8161 acttcttcct ggccgccgac ttcgttcgca cacacaccga cctggccacg atggaagccg
     8221 ccaacgaagc cgcccgccgg gccgtcaacg gcattctgaa ggcctcgggc tcgcggaagc
     8281 ggcgctgcaa agtgtggccg ctggacgagc catggctgtt tgcgccgctg aagtattacg
     8341 acaggttgcg gttccgccgg cttcctgacc gcccccccag ccgcacgctg atctggctcg
     8401 tgctttgcct ggcggtaccg atctggctgg cgatccacct ggcctggagg ttgtacttcg
     8461 cgatcgtgga aggcttgatc gcgcccgtct ggcaatggct gctgcgcacg ttttggaggt
     8521 cgtgaggcgg aagattgatc gtaacccgac gcgtaagcga gggactccct cgctgacgcg
     8581 tcgggttagt ctgcgaagcc gggcatatac agccccgtca ttcgccggcc gttgctgatc
     8641 ttctgctgct gcatccagag atcggcctgg tcgatcagcg cacggccttg gtcgccgccg
     8701 agaagttgtc ctcggcgccg gcgcgcggcg gcggcgaaca aatgcatgtt cgcagcgtcg
     8761 aagccggcgg tcgcgcgctc caagaactga gcggcttccg gctcctgacc gccggcggct
     8821 gccaaggcgg cgcggatccg ctcggccagc ggctgcgacc aggacgcgtc ctcctgctcg
     8881 atccgccgcg cgtcgcggcc ggccgatcgc aggtacggcc gcggatcggc cgcttcgcgg
     8941 gcggcggcca gcgcgctgcg ggcccgcaag tcgcgcatga acagccgcac ggtctgcacg
     9001 cgcaaaaaca gcgatcgctc caggtcgggc cactcgctgg tgacgatctg ccacgccttc
     9061 gcgccgtcgc cttgatacag ggcgatctga atccggccca tcaaccccgt gagatgctga
     9121 acgtgaaatc cgcgatgcga ccaaagggcc atgacctggt gcatgtcgtg ctcggcccct
     9181 tcgggatcgt cggctgccag ccaggtgaga tgcccgacga acgtgcccag gttcgtggcg
     9241 gagtacagat cgccgcgctc gcgggcttcc tggagcagga tcggcagccg ccgcgtcagc
     9301 tcggccactt cgcccaggaa aaagagcgac cagttggcga acgaatgggc cgtatcgagc
     9361 tcccaggcca cgccggtaca gtggtcgcgg aaaacggctt ccgcctgctc cgaaaactgc
     9421 cggccgtctt tccagcggcc ttccatgtag ccggagatgc cccggcacag catcaccatg
     9481 cccagggcgt gcgggtgctc gatgcgatcc gccagtcttc cggccgcctc cagcagccgg
     9541 gccgtggtgc ggcgggccgg caagccgctg gtcgaagtgt gtgcggcttc ccaggccagg
     9601 gctcgcgcaa tgcggtacgg ctcgccgctg ccgagggcca agaggaggtt gcgggactga
     9661 aaatcggccc cgcgaatcgt atcgacaatg ctgaggccca ccgcggccga ccagcaaatg
     9721 tcgatgcggg tcagcgcctc cgccgaaatc tggctgctgt cgcgctcctt gaatcccagg
     9781 ccccgcagcc agagctgcgt tcgccgccag agcagcgacc agagcgcttg ccggggcgtg
     9841 cccgccaggc ggaggccgac actgtccagc accgtgccca gagcttgcag cccatcgtcg
     9901 atatgtccgc tgatgagcga ttgcatcgcg gcccgccgcc gcagctccag gctctccgca
     9961 gccggagcgc cgtccgcggc tgccaggtat tcgcgggcgg ccccagcgcc gcggcccgcg
    10021 ttggccaggg cgtcgcccaa tcgggtgcgc aggccgcggc gatcgggcgc atcattcggc
    10081 tccagatcca gcgccagccg atacagccgg gccgcgcgat cgaacgccag cgccgcggcg
    10141 gcccggtccg ccgccacggc gtagtaatgg ccggcgcggg ccggttcgcc agccgcttgc
    10201 aggtggctgg ccaaagcttc cggatcggca tgggccgagg cttcgagcga ggcggccaac
    10261 cgccggtgac aatcttggag ctcggccgca gacaagtgct cgaccaacgt ctcgcggacg
    10321 cggtcgtgat agacctccat ctcgtcgtgg tcccccgggc cggtgctgcg cacgaggtag
    10381 tccgcacgta gcgtcttcaa ggccatgcgc tcggagggcg ccagcgcggc ggc
//
LOCUS       DASUGB010000017         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1668980, whole genome shotgun sequence.
ACCESSION   DASUGB010000017 DASUGB010000000
VERSION     DASUGB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668980"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFV87_00330"
     CDS             complement(<1..689)
                     /locus_tag="VFV87_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5102224.1"
                     /translation="MIELIDFGKDYGDFRAVESLSLKIDAGEMFGFIGPNGAGKSTTI
                     RFLATLLKASRGEGIVNGHSVTQNPMAVRHSVGYMPDNFGVYDGMKVWEFLDFFAVAY
                     KIGKGKRKQVINDVLELLDLGHKRDDYVNGLSRGMKQRLCMAKTLVHDPPVLILDEPT
                     SGLDPRARIEVKALFKELKKMGKTILISSHILSELADCCTSIGIIERGQLLMHGPIDE
                     VYRRIRGNRMI"
     gene            complement(721..>2208)
                     /locus_tag="VFV87_00335"
     CDS             complement(721..>2208)
                     /locus_tag="VFV87_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102225.1"
                     /translation="EPRIGSTLRYFSRDIGAPGNTAANATAANAVPEEAAGGTAAAAG
                     MPTPPVAVSGTPVMGSPPINYGQFGMGYGDYGPQQRPETWQVHPQLDDWHFLGYESSP
                     VTGVAAWNDQGAASSAARQSLAEAAGIEIPQADFVIKVLAIYLLVLVPLNWLVFWLMG
                     RVEWAWLAAPLIAIVGAGAVIRLAQLDIGFARSRTEIAILEVQGNYERAHLTRYTALY
                     TSLSSTYALGFEDQSALALPFPSGKRDPSPFEFRTTTDVAFRRDKEATLDGVKVSSNS
                     TGMVHSEQMYPLGDDPKLTETLALVGDDAKGYSLRNTTDLTIRDIGIFRRGDNVRGAD
                     NKLVSKIETAYIAELKPQTSAPLAFRPLTGNTVWLPEWSSQPIFATPNSALPEDQKGI
                     VRLTRLATLATQQLRLIPGDVRLVGWTEQPLRGLQIRPAAPQNATYTLVLAHLFRGAL
                     PPVKPDKNVAEDYFEPAIEPEPEPDPNAPLELTPEAEGLNPGLGP"
BASE COUNT          378 a          679 c          726 g          425 t
ORIGIN      
        1 tcgatcatgc ggttgccgcg aatccgccga tagacttcgt cgatcggacc gtgcatcaaa
       61 agctgcccgc gctcgatgat gccgatgctc gtgcagcagt cggccagctc cgaaagaatg
      121 tggctgctga tgaggatcgt cttgcccatc ttcttcagct ctttgaagag ggctttgact
      181 tcgatccgcg cccgcggatc gaggccgctc gtcggctcgt cgaggatcag cacgggcggg
      241 tcgtggacga gcgttttggc catgcacagg cgctgcttca tgccgcgcga gaggccgttg
      301 acatagtcgt cgcgcttgtg gcccagatcg agcagctcca gcacgtcatt gatgacctgc
      361 ttccgcttgc ctttgccgat cttgtaggcc accgcgaaga aatcgaggaa ctcccagacc
      421 ttcatgccgt cgtagacgcc gaagttgtcc ggcatgtacc cgacgctgtg gcggacggcc
      481 atcgggttct gcgtcacact gtggccgttg acgatccctt cgccgcggct ggccttcaag
      541 agcgtggcca ggaaacggat cgtcgtgctc ttgcccgcgc cgttaggacc gatgaagccg
      601 aacatctcgc cggcgtcgat cttgaggctc agcgactcga ccgcgcggaa gtcgccgtag
      661 tctttgccga agtcgatcag ttcaatcatg ggcaaccggt tggaaacaaa gggctaaggg
      721 ttagggacca aggccaggat tcaagccttc ggcttcggga gtcaactcga gcggcgcgtt
      781 tggatcgggt tcaggttcgg gttcgatggc cggttcgaag taatcttccg caacgttttt
      841 atcaggcttg actggcggca aagcgccgcg gaacaggtgg gcgaggacga gcgtgtaggt
      901 cgcattttgt ggtgcggcgg gacgaatctg cagaccccgc agcggctgct cggtccagcc
      961 caccagccgc acgtcgcccg gaatcaggcg gagctgctgc gtggccaggg tcgccaggcg
     1021 ggtcagacgg acgattccct tctggtcctc cggcagggcc gagttcggcg tcgcgaagat
     1081 cggctggctg ctccattccg gcagccagac cgtattgccg gtaagcggcc ggaaggccaa
     1141 tggcgcgctg gtttgtggct tcagctcggc gatgtaggcg gtttcgatct tggaaacgag
     1201 cttgttatcc gcgccgcgga cattgtcgcc gcggcggaag atgccaatat cgcgaatcgt
     1261 caggtcggtc gtgttcctca gtgagtagcc cttggcatcg tcgcccacca gcgccagcgt
     1321 ctcggtcagc ttggggtcgt cgcccagcgg gtacatctgc tcgctgtgga ccatgccggt
     1381 ggagttcgac gaaaccttca cgccatccag cgtcgcttct ttgtcgcggc ggaacgccac
     1441 atcggtcgtg gtgcgaaact cgaacggcga cgggtcgcgc ttgcccgatg ggaacggcag
     1501 cgctagagcg ctttggtcct cgaaacccaa ggcgtacgtg ctcgacaggg aggtatagag
     1561 cgccgtgtac cgggtgaggt gcgctcgctc gtaattcccc tgaacttcca ggatcgcaat
     1621 ttccgtccgg ctgcgggcaa agccgatatc gagctgtgcc agccggatca cggctcctgc
     1681 tccgacgatg gcgatcagcg gcgcggcgag ccaggcccat tccacgcggc ccatcagcca
     1741 gaataccagc cagttcagcg gcacgagcac cagcagatag atggccagca ctttgatgac
     1801 aaaatcggcc tggggaatct cgatgcccgc ggcctctgcc agcgactgcc gggccgcgga
     1861 cgaggcggcg ccttgatcgt tccaggcggc cacacccgtg accggcgacg actcgtagcc
     1921 cagaaagtgc caatcgtcga gttgcgggtg gacctgccag gtttcgggcc gctgctgcgg
     1981 tccgtagtcg ccatacccca ttccgaactg gccataattg atcggcggcg agcccatgac
     2041 gggcgttccg ctgacggcca cgggcggcgt cggcatcccg gcagcagcgg ctgttccacc
     2101 ggccgcctcc tcaggaacgg cattcgccgc ggtggcattc gcggcggtgt ttccaggggc
     2161 gccgatatcg cggctgaaat agcggagcgt gctgccgatc cggggctc
//
LOCUS       DASUGB010000018         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_557783, whole genome shotgun sequence.
ACCESSION   DASUGB010000018 DASUGB010000000
VERSION     DASUGB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557783"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFV87_00340"
     CDS             <1..792
                     /locus_tag="VFV87_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5102226.1"
                     /translation="ISLQLDVLKPKAESDKPRPCIVWIHGGGWQNGNKSSGLARLAGY
                     VASGDYVGVSVGYRLTDKGSWPAQIHDCKAAIRWIRANAEKLGIDPNKIGVWGSSAGG
                     HLVSHLGTSGDVKEVEGELGTTGVSSRVACVVDFCGPSDFPSFQLTSGAQGPVSKLLG
                     GVPKDKPDAAKQASPITFVTKDDPPFLIVHGTDDRTVPFDQADRFHDAQKKAGVDSTF
                     IKIEGGGHGIGGPEVEARVRAFFEKHLLGKDVEVSAEPIKQAARE"
     gene            complement(889..1320)
                     /locus_tag="VFV87_00345"
     CDS             complement(889..1320)
                     /locus_tag="VFV87_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX5102227.1"
                     /translation="MRARLLIVLSLAVVGLAGFAAGLSIGEEKKVNTRVYELRTYTTL
                     PGRLPALNKRFAEHTMKLFEKHGMKNEMYWVPTDAERKDNTLIYIVSHESQEAAEKSW
                     KEFQADPEWQAARDASEKDGKILAKAPERVFMTLTDYSPGK"
     gene            1518..2411
                     /locus_tag="VFV87_00350"
     CDS             1518..2411
                     /locus_tag="VFV87_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX5102228.1"
                     /translation="MTTAVTNAAGPPWTDKAPAKPRLVLLGSGDRAGVLEEAARLRPV
                     IAEHLQLVHEDLYYQTDLTPIEADLAVVLGGDGSILRAALQMRERQIPILGVNLGKLG
                     FLAEVKPDEVASVLPDVAAGNVRIVEHLMFRCTVTRGGEPVAETLGLNEVAIFAGQPF
                     SMLTANLYVDADLATTYACDGLIISTPVGSTAHSLSAGGPILRKTLAAFVICPIAPHT
                     MTVRPVVDTADRTYEVVVPQPNEGTTVVVDGRPLWKLAASDRVRVVRAEPTFKLIEVR
                     GQNYYRTLRDKLGWAGNLYRK"
     gene            2557..>2782
                     /locus_tag="VFV87_00355"
     CDS             2557..>2782
                     /locus_tag="VFV87_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102229.1"
                     /translation="MKSALSLPTLLTATILVILFGACVVAAARGDETPAATTRTISKP
                     DKDLAAELDKAKDKLAPSYTLAYKFAPGDQV"
BASE COUNT          513 a          872 c          893 g          504 t
ORIGIN      
        1 atcagcctgc agctcgatgt gctcaagcca aaggcggaaa gcgacaagcc gcggccttgt
       61 atcgtgtgga ttcatggcgg cggctggcaa aatggcaaca agtcgagcgg cctggcgcgg
      121 ctggcgggct atgtggcgag cggcgactat gtcggcgtat ccgtcggcta tcggctgacg
      181 gacaagggaa gctggccggc ccagattcat gattgcaaag ccgcgatccg ctggattcgg
      241 gcgaacgctg agaagctcgg catcgatccg aacaagatcg gcgtctgggg ctcgtcggcc
      301 ggcgggcatc tagtgagcca cttaggcacg tctggcgacg tgaaggaagt cgaaggcgag
      361 ctggggacga ccggcgtcag cagccgcgtg gcgtgcgtcg tcgatttttg cgggccgagc
      421 gatttcccga gttttcagct aaccagcggc gcgcaaggcc cggtttccaa gctgctcggc
      481 ggcgtcccca aggacaagcc ggacgcggca aagcaagcct caccgatcac gttcgtcacg
      541 aaggacgatc cgccgttcct catcgtccac ggcaccgacg accgcacggt tcctttcgat
      601 caggccgatc gcttccacga tgcccagaag aaggccggcg tcgatagcac cttcatcaag
      661 atcgaaggcg gcggccacgg catcggcggc cccgaagtcg aggcccgggt acgggcgttc
      721 ttcgagaagc acctgctggg caaggacgtg gaagtgtcgg cggagccgat caagcaggcg
      781 gcccgcgagt gaatgctctt agccggaagc aaacttgctc ccggcgcgcg gccggttgat
      841 cgtccactcg caattctccg gaaccaggtt tggtcccggc taagggcatt atttgccggg
      901 cgagtagtcc gtgagcgtca tgaagacccg ctcgggggct ttggccagaa tcttgccgtc
      961 tttctcgctg gcgtcgcggg cggcttgcca ctcgggatcg gcctggaatt ccttccagct
     1021 cttctcggcg gcttcctggc tctcgtgcga gacgatgtag atcagcgtgt tgtccttccg
     1081 ctcggcgtcg gtcggaaccc aatacatctc gttcttcatg ccgtgcttct cgaagagctt
     1141 catcgtgtgc tcggcgaacc gcttatttag cgccggcagc cggccgggaa gcgtggtgta
     1201 ggtccgcaat tcataaacgc gcgtattcac ctttttctcc tctccaatcg acagccccgc
     1261 ggcgaatccc gccagaccga caacagccag cgacaaaacg atcaacaacc tggcacgcat
     1321 ggtgatactc ctggttttaa ggttcaagga tgactgcacg atcttatcac gccgcgccgt
     1381 gcgaggtgca atcgtaggct tctcgctccg cgagaagcga acgactttcc atgcgcaggg
     1441 acgatgcgcc tggcgacgtc cattgcttgt cgcggagcga caagcctacg atgtgggccg
     1501 catggtacga tggatgcatg acgactgctg tcacaaatgc cgctggcccg ccctggactg
     1561 acaaagcccc agccaagccg cggctggtgc tgctgggctc cggcgaccgg gcgggcgtgc
     1621 tggaggaagc ggctcgcttg cggccggtga ttgccgagca tctgcagctc gtgcacgagg
     1681 acctctatta ccagaccgac ctgacgccga tcgaagccga cctggcggtg gtcctgggcg
     1741 gcgacggctc gatcctgcgg gcggccctgc agatgcggga gcggcagatt ccgatcctgg
     1801 gcgtgaacct gggaaagctg ggattcctgg cggaagtgaa gcccgacgag gttgctagcg
     1861 tcctgcccga tgtggccgcg ggcaacgtgc ggatcgtcga gcacctcatg ttccgctgca
     1921 cggtcactcg cggaggcgag ccagttgccg agaccctcgg cctcaacgag gtcgcgatct
     1981 tcgccggcca gcccttctcg atgctgaccg ccaatcttta cgttgacgcc gatttggcaa
     2041 ccacttatgc ttgcgacggc ctgatcatca gcacgcccgt gggctccacg gcccacagcc
     2101 tttcggcggg cgggccgatc ctgcggaaga cgctggccgc cttcgtcatc tgcccgatcg
     2161 ccccgcacac catgaccgtg cggccggtcg tcgacacggc tgaccgcacc tacgaagttg
     2221 tggtcccgca gccaaacgaa ggaaccacgg tggtcgtcga tggccgcccg ctctggaagc
     2281 tggcggcgag cgaccgcgtg cgggtggtgc gggccgagcc cacgttcaag ctgatcgagg
     2341 tccgcgggca gaactattac cgcacactcc gcgacaagct cggctgggca gggaatttgt
     2401 accgcaagta aagtagccgt ctcgctccgc gagacgagcc catttttgaa gtcgtttcgc
     2461 tctgctggac gagcccaatg cattgggcaa atcacgcgga gcgtgattgc tacgtaggtc
     2521 tcgctccgcg agacgagcac gcacggaggc gaagaaatga aatccgcact ttcccttccg
     2581 accctgctga cggcgacgat tctcgtcatt ctctttgggg cctgcgtggt ggcggctgcc
     2641 cgcggcgatg agacgccggc ggcaaccacc aggacaatat cgaaacccga taaagacctg
     2701 gctgctgagc tcgacaaggc caaggacaag ctcgcgccaa gctacacgct ggcctacaag
     2761 ttcgcgccgg gcgaccaggt cc
//
LOCUS       DASUGB010000019         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1113555, whole genome shotgun sequence.
ACCESSION   DASUGB010000019 DASUGB010000000
VERSION     DASUGB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113555"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFV87_00360"
     CDS             <1..1281
                     /locus_tag="VFV87_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102230.1"
                     /translation="ELVDEAILRQRISEADRLVRIAEGQAKTLSDNKLRAAMTTQRLE
                     VEKLVKQWETVEKARQALAGNPDDAAAALADGRYRCLAEGDWKAGLELIARGSDERLK
                     HAAALDLAGTSQEIAAAKIGDAWHQIASSDESLAGFFARALHWYRLQAGGSETLEQAR
                     IQQRMEQIEALKLPPRLLDQVNVATANAPLPPFYPAFTRVYSFGPVDLFNFVQRTDLS
                     ASPWAATNDMPPALYSDADAALARLPARYPPPREYQLTIRVRRGRPTSGAAPAFPLAE
                     PEPTGPFVIGLVGPKSPFLACLDLPIGGGEFGSFLTTGEAAAPQDNPTLRRLSSAQIS
                     GRFRGESTIVCQVRRRSVSVMVDSTEACRYEGDLAKLSLPKEWSINDPRSLFIGSHKC
                     CYSVTSWMLEPLPAESRVPASTAGFSAGAAPIDF"
     gene            1468..>2227
                     /locus_tag="VFV87_00365"
     CDS             1468..>2227
                     /locus_tag="VFV87_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5102231.1"
                     /translation="MRARHPRCLPLVESLEPRHLLAGVSLTDGLLEITGTGGNDDIRV
                     THPGEQIVVRLNGQKFTFAACAVTGISIDAGAGNDRVDVANKILIDAYILGGEGNDTI
                     LGGGGNDTIIGGSGNDDINGRGGNDGISGDGGNDVLRGGAGDDTLGGGDGNDDLRGGA
                     GDDSLSGEGGDDLLNGGAGNDTLSGGEGNDDVRGGQGDDSLSGDAGNDVLQAGAGNDY
                     LSGGAGNDLLNGHAGDDVLAGGDGEDTLQGGAGND"
BASE COUNT          402 a          673 c          783 g          369 t
ORIGIN      
        1 gagctggtgg acgaagcgat tcttcgccag cgaatttccg aagccgacag gctcgtgcgg
       61 atcgccgagg gccaggccaa gacgctcagc gacaacaagt tgcgggccgc gatgacgacc
      121 cagcggctgg aggtggagaa gctcgtcaag caatgggaga cggtcgagaa ggcgcggcaa
      181 gcgctggccg gcaacccgga cgatgccgcg gcggcgctgg cggatggccg ctaccgctgc
      241 ctggcggaag gcgattggaa ggcgggcctg gaattgatcg ctcgcggcag cgacgagcgg
      301 ctgaagcatg ccgccgcgct ggatctcgcc ggcacgagcc aggagattgc ggcggcgaag
      361 atcggtgacg cctggcacca gatcgcctca tccgatgaat cgctggctgg gttcttcgcc
      421 cgagctttgc actggtaccg cctgcaagcc ggcgggagcg aaacgctcga gcaggcccgc
      481 atccagcagc ggatggagca aatcgaggcg ctgaagctgc caccccggct cttggaccag
      541 gtgaacgtcg ccacagccaa tgcacccttg ccgccgtttt atccggcgtt tacccgggtt
      601 tattcgttcg ggccggtcga tctgttcaat tttgtgcagc gaaccgatct gtcggcttcg
      661 ccctgggccg cgacgaacga catgccgccg gcgctgtatt cggatgccga cgcggcactg
      721 gcccggctgc cggcgcgtta tccgccgccg cgggagtacc aactgacaat ccgcgttcgc
      781 cgcgggcggc cgacgagcgg cgcggcgccg gcatttccgc ttgccgagcc ggagccgacg
      841 gggccgttcg tcattggcct ggtgggaccg aaatcgccgt ttctggcttg tctcgatttg
      901 ccgatcggcg gcggcgagtt cgggagcttt cttacaacgg gcgaggccgc ggcgccacag
      961 gacaatccga ccctccgccg actgagcagt gcccaaatca gcgggcggtt ccgcggggag
     1021 agcacgatcg tgtgccaggt gcgccgccgc agcgtaagcg tcatggtcga ttcgaccgag
     1081 gcttgccgtt acgagggaga ccttgcgaaa ctctcgctgc ccaaggagtg gtcgatcaac
     1141 gacccccgct cgctgttcat cggctcgcac aagtgctgtt actcggtcac gagctggatg
     1201 ctggaaccgc tgccggccga aagtcgagta cctgcgtcga cggcgggatt ttcagctggt
     1261 gctgcaccca ttgattttta ggcgggagaa gtccctcgtt gttgctcgca ggagcggatt
     1321 gtcgacgaaa gttgacaatc ccgatatccg acctagggtt tagttggctc acccgctcct
     1381 gcgtttaggg ggttcgcctc ctggtcgcag gctcgtcttg gcggacgtga gccgagccac
     1441 tcaccatttc ctcgcagggg cgaagccatg cgcgcccgtc atcctcgctg tttgccgctt
     1501 gttgagtccc tggaaccgcg acatctgctg gcgggcgtgt cgctgacgga tggattgctg
     1561 gaaatcaccg gcaccggcgg gaacgacgac atccgggtga cgcatccggg cgagcaaatc
     1621 gtcgtgcggt tgaacggcca aaaattcacg tttgcggcgt gcgccgtaac gggcatctcg
     1681 attgatgcgg gcgccggcaa cgatcgcgtg gacgtggcca acaagatcct gatcgacgcc
     1741 tacattcttg gcggcgaagg gaacgacacg atccttggag gcggtggtaa cgacacaatc
     1801 atcggcggca gcggcaacga cgatatcaac ggccgcggcg gcaatgacgg gatcagcggc
     1861 gacggcggca acgacgtgct gcgcggcggg gccggcgacg acacactcgg cggcggcgat
     1921 ggaaatgacg atctgcgcgg cggcgcaggc gatgacagcc tcagcggcga gggtggggac
     1981 gacttattga atggcggcgc cggcaacgac acactgagcg gcggcgaggg aaatgatgat
     2041 gtccgcggag gccagggcga tgacagcctc agcggcgacg cgggcaacga cgtgctgcag
     2101 gcgggcgcgg gcaacgatta cttgagtggc ggcgcaggca acgatcttct taatggccac
     2161 gctggcgacg acgtcctggc cggcggcgat ggcgaagaca cgctccaagg cggcgcgggc
     2221 aatgaca
//
LOCUS       DASUGB010000020         7067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_419590, whole genome shotgun sequence.
ACCESSION   DASUGB010000020 DASUGB010000000
VERSION     DASUGB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419590"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1752
                     /locus_tag="VFV87_00370"
     CDS             268..1752
                     /locus_tag="VFV87_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102232.1"
                     /translation="MSNSLRVLLTASVLIAGCEAAVPAAAPQKANPAPTATKETPTTA
                     VAPPAAASKDFVPAQPIQVGPARAPLEDLARQAEAQQFDLPALDEGKIAAAGIRRLDG
                     QHITIYTDLPEAAGQAVQELPQVFDAAVPLWCAYFGVDPATVEKWKIVGCVMVEKGRF
                     QGAGLYSDNLPDFPNGYSAGSQVWLYDQPSDYYRRHLLLHEGTHAFMTRWLGGAGPPW
                     YMEGMAELLGTHEWAGGKLTLGYLPRTKEEVPYWGRVKIVKDEFAANRGMTLDLIMNY
                     DSRAHLRNEPYGWCWAAATFFDQHPLTQAAFRDLRSQVKDRTVDFNNRFRGRLKDHLW
                     EIDEDWLLFVLQCEYGYDVARAAVVRKPVGDLPAAGASVSIAADRGWQSSGFKLMAGR
                     TYRLTAEGRYQVGNSPKPWPCEPGGVTIRYVAGQPLGMLLAATSDEEMPARPITPLAN
                     PQPIGVSATLTPETTGTLYLRINEAPSGLADNAGTLSVRIEPQP"
     gene            complement(1841..4201)
                     /locus_tag="VFV87_00375"
     CDS             complement(1841..4201)
                     /locus_tag="VFV87_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5102233.1"
                     /translation="MAFVNLSLLAGGLFVAIPVVLHLIMRQRPKPMVFPALRFIQQRR
                     IANQRRLQLRHWLLLALRCGAIGLVALALARPSVASAAMSSWITAGLLAAGTLTALLL
                     SVAAFSRGLPKYAAGGFAVVAALFLLATLFVGGRALAGRSPIMGDQEAPVAAVLVIDT
                     SPRMQYRHENKTRLEAAQEMAQWLIKQLPADSEIAVADSRPGSGAFAVDRAAAANSIE
                     RLRPAGTPRPLVEVLESSVALVKEKSQSRKEIYVLTDLTQSAWKAPAASDLAGVLKEN
                     DNVLLYVIDVGVLQPQNFALEGLTLPSDLLPAGAELAIETQAAASGVGGERTVELQLE
                     VPDPTLPIIRDGKPVLPKSVRRGSQTVKLDAGESQRVRFSILGLEPGVHQGLVRLLGE
                     DGLALDDVRYFAVEVQPAWPVLIVTPPDVSPRYLVEALAPRELRETGQARFRCETIEQ
                     SRLASQELGDFRAVALLDPQPLTPDAWNKLAEFAERGGGVAIFLGHNAQPPSSFQDPA
                     AVKVLGGKLTRQTRTAGDVYLAPQSYDHPALGVFRQLETSIPWDRFPVHYHWNLDELS
                     PDARTIVPYSNGKPAVVENRLGRGSVLAMTTPISDPPRPAGYAAWNELATGEDAWPCF
                     VLVNEMLVHLVKSGQTRLNLLAGETAVLPNDPAEFPERYQLFTPLDQPQDVLARDGRV
                     TIRFTDNPGAYRLRGQKPGVGPIVRGFAVNLPADSSDLSRLPREKLDELLGKGRYQLA
                     RSREEIDRAVGNDRIGSEFYPLLAVLLVVTLGLEHVLANRFYRKEE"
     gene            4270..4971
                     /locus_tag="VFV87_00380"
     CDS             4270..4971
                     /locus_tag="VFV87_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX5102234.1"
                     /translation="MQITARAARRTVLLGAAAILVGKVTLSVVAGYRDYLPPNFDSDF
                     LLGREAYFWGPYSGAFYAHLVSGPLSLVLGTLLISQRFRQMAPRWHRRLGRVQVACIL
                     LLLTPSGLWMARYAFTGSVAGAGLGSLAIATALCTVLGWRAAVGRRFDDHRCWMWRTY
                     LLLCSAVVIRLIGGAASVLALDALWLYPLAAWASWLVPLAAFEVWRWSNRRREPRTKH
                     RSNTDDQGSFSIIRV"
     gene            complement(4990..5760)
                     /locus_tag="VFV87_00385"
     CDS             complement(4990..5760)
                     /locus_tag="VFV87_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102235.1"
                     /translation="MNVNPYESPETPGAPPEKKAGRRFTLVELLVVVVLIGLLIACLL
                     PARRSATEAGRRMSCQNNLHEIGLALEQYHDVYGSFPPAYTVDADGKRLHSWRTLILP
                     YLEEKFLYDKIDLSKPWNDPANQFVLEARIGTYQCPSADIPKNHTTYLVVVANSGCFR
                     GAEPRKREEVTEDHNRALLVIEVPADRAVPWMSPHDADVPSFLTAVAADKPAHPSGMQ
                     VVCVDGTIHTLSADIEPNKLRAMISVAGDDETDAAGEK"
     gene            6144..>7067
                     /locus_tag="VFV87_00390"
     CDS             6144..>7067
                     /locus_tag="VFV87_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102236.1"
                     /translation="MPQLRDRRTDGPAYRRVWWAFRLALFAAGVAALAHAGSPYHWED
                     WFASGGPWSSARAQEKADGDAADQPAPATPAEAFRFDVPLPITGLVDQQVTSSVERAL
                     RKLPQGGPRPVFIFEFRPKANSTGEGSDFGRALTLARYLAGDRLSGVRTVAWLPNSVK
                     GHAVLPVLACEQIVMHKEAELGAAGIDEKTIDPALRSGYTEIAERRRTVPTAIALGML
                     DKSLAVYKVTTPDGVRYETEEELKKLRDGGLVTKEDNLFQPGDEHVLTGQEMRFELGF
                     ASHLAEDRRSLAAALQLPLRALSQNLAPEEGW"
BASE COUNT         1191 a         2335 c         2352 g         1189 t
ORIGIN      
        1 tgagagctgc acgatgagca cgcccaccac agccacgatc gccgtggccc agtgcgcggg
       61 aagtaatcgc ggcgcttggt gggaaaggtt gggctgcgtg gtcatgcggc gccatgttaa
      121 cccgaagcgt aagcgaggga atcccactcg caaacaaggc cgtttttcgg ggacttccgc
      181 gcttacgctt cgggttggtg tgagcggggg ccggcgggct agaatacgat agttggattc
      241 ccggagactg ccaaacccag aggcgcgatg tcgaattcgt tgcgagtcct gttgacggca
      301 tcggtgctga tcgccggctg cgaggcggcg gttcccgcgg ctgcgcccca gaaggcgaat
      361 ccggcgccga cggcgacgaa ggagaccccg acgacagccg tcgcgccgcc agcagcggca
      421 tcgaaggact ttgtccccgc ccagcccatc caggtcggcc ctgcccgagc tcccttggaa
      481 gacctcgccc gccaggccga ggcccaacaa ttcgatcttc ccgcgctgga cgaaggcaaa
      541 atcgccgctg ccggcatccg ccgcctcgat ggccagcaca tcacgatcta taccgacctg
      601 ccggaagccg ccggccaggc agtccaggag ctgccgcagg ttttcgacgc cgccgtgcca
      661 ctgtggtgcg cgtatttcgg cgtcgatccg gccacggtgg agaagtggaa gatcgtcggc
      721 tgcgtgatgg tcgaaaaagg gcgattccaa ggcgccggtt tgtactcgga caacctgccc
      781 gatttcccca acggctattc cgcgggctcg caagtctggc tctacgacca gccgagcgac
      841 tactaccgcc gccatctcct gttgcacgaa ggcacgcacg ccttcatgac gcgctggctc
      901 ggcggggccg gccccccatg gtacatggaa ggcatggccg aattgctcgg cacgcacgag
      961 tgggcgggcg gcaagctcac gctcggctat ttgccgcgga ccaaggagga agtcccctac
     1021 tggggccgcg tcaagatcgt caaagacgag ttcgccgcca accgcggcat gacgctcgat
     1081 ttgattatga actacgacag ccgggcccac ctgcggaacg agccctacgg ctggtgctgg
     1141 gcggcggcaa cgtttttcga ccagcatccg ctcacgcagg ccgcattccg cgatctccgc
     1201 tcgcaggtca aagaccgcac ggtggatttc aacaaccggt ttcgcgggcg gctaaaggac
     1261 catttgtggg agatcgacga ggactggctg ctattcgtcc tgcagtgcga gtacggctac
     1321 gatgtcgcgc gggccgctgt cgtccgcaag ccggtcggcg atctgcccgc ggccggtgcg
     1381 agcgtgtcga tcgcggccga ccgcggctgg caatcgagcg ggttcaagct gatggccggc
     1441 cgcacgtacc ggctcaccgc ggagggccgc taccaggtcg gcaattcgcc gaagccctgg
     1501 ccgtgcgagc ccggcggcgt gacgatccgc tacgtagcgg ggcagccgct cggcatgctt
     1561 ctggccgcaa ccagcgacga ggaaatgccg gccaggccaa tcacgccgct cgccaatccg
     1621 cagccgatcg gcgtcagcgc cacgctcacg cccgagacga ccggcacgct ctatctgcgg
     1681 atcaacgaag cgccctccgg cctggccgac aacgccggca cgctgagcgt ccgcatcgag
     1741 ccgcagccgt gaataccaac ccgacgcgta agcgagggaa tccctcgctt tcgcgtcggg
     1801 taagtgagtg actccctcgc tgacgcttcg ggttagtgtc ctactcttcc ttccgataaa
     1861 accggttcgc cagcacgtgc tccaggccca gggtcacgac cagcagcacg gccaggagcg
     1921 gataaaattc gctgccgatc cggtcgttcc cgaccgcccg gtcgatctct tcgcggctgc
     1981 gggccagctg gtagcgcccc ttgcccagca actcgtcgag cttctcgcgc ggcaggcgcg
     2041 acagatcgct cgaatcggcc ggcaagttca ccgcgaagcc gcggacgatt ggccccacac
     2101 ctggcttctg cccgcgaagg cgatacgcgc ccggattatc ggtgaagcga atcgtcacgc
     2161 ggccgtcgcg ggccagcaca tcctgcggct gatcgagggg cgtgaatagc tggtaccgct
     2221 ccgggaactc cgccgggtcg ttgggcagca ccgccgtctc gccggccagc agattcagcc
     2281 gcgtctggcc gctcttgacc aggtgcacga gcatctcgtt aacgagcacg aagcacggcc
     2341 aggcgtcctc gccggtcgcc agctcgttcc atgccgcata acccgcgggc cgcgggggat
     2401 cggatatcgg cgtcgtcatc gccagcacgc tcccgcggcc caggcgattt tcgaccaccg
     2461 ccggcttgcc gttgctatag gggacaatcg tgcgggcgtc cggcgaaagc tcgtcgaggt
     2521 tccagtgata gtgcaccggg aagcggtccc acggaatgct cgtttcaagc tggcggaaaa
     2581 cgccgagagc cggatggtcg tacgactgcg gcgcgagata gacgtcgccc gcggtccgcg
     2641 tctgccgcgt cagcttgccg ccgagcacct tgaccgccgc cggatcctgg aacgacgagg
     2701 gcggctgggc gttgtgtccc aggaaaatgg ccacgccacc gcctcgctcg gcaaattcgg
     2761 ccaatttgtt ccacgcatcg ggcgtcaacg gctgcgggtc gagcagggcg acggcgcgaa
     2821 agtcacccag ctcctggctg gcgaggcgtg actgctcgat cgtctcgcag cggaaccgcg
     2881 cctggcccgt ctcgcgcagc tcgcgcggcg cgagcgcctc gaccagatac cgcggcgaaa
     2941 catccggcgg cgtgacgatc agcactggcc acgccggctg tacttcgaca gcgaagtatc
     3001 gcacatcgtc cagtgccagg ccgtcttcgc cgagcagccg gaccaggccc tgatgcacgc
     3061 ctggctccag gccgaggatc gaaaaccgca cccgctgcga ttcccccgca tcgagcttca
     3121 ccgtctggct gccgcggcga accgacttgg gcagcaccgg cttgccgtcg cgaatgatcg
     3181 gcagcgttgg gtcgggtact tcgagctgca attccaccgt tcgctcgccg ccgacgccgc
     3241 tcgccgcggc ctgcgtctcg atcgccagct ccgcccccgc cggcaacagg tcgctgggca
     3301 gcgtcagccc ttccaaagcg aaattctgcg gctgcaggac gccgacatcg atcacataga
     3361 gcagcacgtt gtcgttttct ttgagcacgc cggccaaatc gctcgcggcc ggcgctttcc
     3421 aggccgactg cgtgaggtcg gtcaggacgt aaatctcttt gcgcgactgc gatttttcct
     3481 tgacgagcgc caccgacgat tcgagcacct ccaccagcgg gcgcggcgtg ccggcgggcc
     3541 gcagccgttc gatcgaattg gccgcggcgg cccgatcgac ggcgaacgcg ccgctgccgg
     3601 ggcgggaatc ggcgacggcg atctcgctgt cggccgggag ctgcttgatc agccactggg
     3661 ccatttcctg ggccgcctcc aggcgggtct tgttctcgtg gcgatactgc atccgcggcg
     3721 aggtgtcgat caccagtacc gcggcgaccg gcgcttcctg gtcgcccatg atcggcgagc
     3781 gtccggccag cgcccggccg ccaacgaaga gcgtcgccaa caggaacagt gccgcgacga
     3841 ccgcaaaccc gccggcggca tatttgggca gcccgcgcga aaaggcggcg accgacagca
     3901 gcagcgccgt cagcgtccca gccgccagca gtcccgccgt gatccaactc gacatagccg
     3961 ccgacgctac gctcggccgg gccagtgcca aggccaccag tccgatcgcc ccgcaccgca
     4021 gcgcgaggag cagccaatgc cgaagctgca gccgccgctg attggcgatc cgccgttgct
     4081 ggatgaaccg cagggccgga aagaccatcg gcttggggcg ctggcgcatg atcaggtgca
     4141 gcacgaccgg aatcgcgaca aacagcccgc cggccagcag cgataagttg acaaaggcca
     4201 tgcaccaggt tcctgaacgc gacttctcca tattagtgcg cgatggcgac gggctggtag
     4261 gatggcaaaa tgcagatcac agcgcgagcg gcgaggcgga cggtgctgct gggggcggcg
     4321 gcgatcctcg tgggaaaggt caccctgagc gttgtcgccg gctatcgcga ctatctgccg
     4381 ccgaattttg actcggattt cctgctgggg cgggaagcgt acttctgggg gccgtatagc
     4441 ggggcgttct acgcgcatct ggtgtcgggg ccgctgtcgc ttgtgctggg cacgctgctc
     4501 atcagccagc gattccggca gatggccccg cgctggcacc ggcggctggg gcgtgtgcag
     4561 gtcgcctgca tcctgctctt gttgacgcct agcggcctgt ggatggcccg gtacgccttt
     4621 acgggctcgg tcgccggggc cgggctgggc tcgctcgcga tcgccaccgc cctgtgcacg
     4681 gtgctgggat ggcgggccgc cgtcggccgc cggttcgacg accaccgctg ctggatgtgg
     4741 cgcacgtacc tgctcctctg ctcggccgtc gtcatccggc tgatcggcgg cgcggcaagc
     4801 gtgctggccc tcgatgccct ctggctctat ccgctggcgg cgtgggcgag ctggctggtg
     4861 ccgctggcgg cgttcgaagt ctggcgctgg agtaatcggc ggcgggagcc acggacgaaa
     4921 cacagatcga acacggatga tcagggatct ttctcaatta tccgtgtttg atctgtgttt
     4981 gatccgtggc tacttctccc ctgcggcgtc cgtttcgtcg tcgccggcaa ccgagatcat
     5041 ggcccgcagt ttgttgggct cgatgtcagc cgaaagtgtg tgaatagtgc catcgacaca
     5101 caccacttgc atgccggaag gatgggccgg cttgtcggcg gcgacggcgg ttaggaacga
     5161 cggaacgtcg gcgtcgtgag gcgacatcca ggggacggca cgatctgccg gcacttcgat
     5221 gaccaggagc gcccggttgt ggtcctcggt gacctcctcc cgctttcgcg gttcggcccc
     5281 acggaagcag ccgctattcg cgacgacaac cagatacgtc gtgtggtttt taggaatatc
     5341 ggcggaagga cattgatatg tcccgatcct ggcctccagc acgaactggt tcgccggatc
     5401 gttccacggc ttggagaggt cgatcttgtc gtacaggaat ttctcttcca ggtacggcag
     5461 gatcagcgtc cgccaactgt gcagccgctt gccatcggca tcgacggtgt acgcgggcgg
     5521 aaagctgccg tacacgtcgt ggtactgttc gagcgccaga ccaatttcgt ggaggttgtt
     5581 ctggcacgac atccggcgcc cggcctccgt cgcactacgt ctggccggca gcaggcaggc
     5641 gatcaacaac ccgatcaaaa ccaccaccac gagcaactcg accagcgtga atctccgtcc
     5701 cgccttcttt tccggcggcg ctcccggagt ctccggactt tcgtaggggt tgacgttcat
     5761 gaaacgaatc cggtacctgc cgtagcctcc ctttcagcat atcaggtccg cgcgactggg
     5821 tttgagaacg cttccgaact ccggcaagca atttgcggca acaacggggc ggccggggcg
     5881 aataaccggt cggaccggag gtccaaccta cgtgaccggt cgcagggggc agcggggcgg
     5941 gtccatgggg ccggcgccgt cgaactcggt cgccgttaac gccgtcctgt taagactttg
     6001 cgcagcccgt cccagactgg ctgcgcggat cattccattc tgccgccggc aggccctata
     6061 attcggagga ccgcacggtc attgccaggc agtggtggcg cgccggctgg gtgaggcaaa
     6121 gtggcgcgtg gaggggtcag gatatgccgc aacttcgtga tcgacggacc gacggcccgg
     6181 cctatcgccg cgtgtggtgg gcgtttcgcc tggcgctctt cgccgctggc gtggcggcgc
     6241 tggcacacgc gggcagcccg tatcactggg aagattggtt tgcgagcggc ggtccgtggt
     6301 cgtccgcccg cgcgcaagag aaagcagatg gcgacgccgc ggaccagccc gcccctgcga
     6361 ccccggccga ggcgttccgc ttcgatgttc ccctgccaat caccggcctg gtcgatcagc
     6421 aggtgaccag cagcgtcgaa cgggccctgc gcaagctgcc ccagggaggc ccgcggccag
     6481 tgttcatttt cgagttccgc ccgaaagcga attccacagg cgaggggagc gatttcggcc
     6541 gcgctttgac gctggcccgc tatctggccg gcgaccgcct gagcggcgtg cggaccgtcg
     6601 cctggctgcc gaattcggtc aaaggccacg ccgtgctgcc ggtgctggcc tgcgagcaga
     6661 tcgtgatgca caaggaggcc gagctgggcg cggcgggcat cgacgaaaag acgatcgatc
     6721 cggcgctccg cagcggttac accgaaatcg ccgagcggcg gcggaccgtg ccgacggcga
     6781 tcgccctggg catgctcgat aaaagcctgg ccgtttataa ggtgacgacg cccgacggcg
     6841 tgcgctatga aaccgaggag gagttgaaga agcttcgcga tggcggcctc gtcaccaagg
     6901 aagacaatct cttccagccg ggcgacgagc acgtcctgac cggacaggaa atgcggttcg
     6961 agcttggctt tgcgagccat ttggccgagg atcggcggtc gctggcggcg gcgttgcagt
     7021 tgccgctgcg ggcgctctcg cagaatcttg cgccggaaga agggtgg
//
LOCUS       DASUGB010000021         6293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_419661, whole genome shotgun sequence.
ACCESSION   DASUGB010000021 DASUGB010000000
VERSION     DASUGB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419661"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFV87_00395"
     CDS             complement(<1..258)
                     /locus_tag="VFV87_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5102237.1"
                     /translation="MQGLIWRANTYLLVEDQAQARDLCREAIALDPQHDGARLMLVRL
                     LVNREPKEAQDHLQMLLRRDPGAPQLLYHQALIDRNLGRLAD"
     gene            280..531
                     /locus_tag="VFV87_00400"
     CDS             280..531
                     /locus_tag="VFV87_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102238.1"
                     /translation="MPFPPVCQLQHSVRLERGQAPGDLCFSDLRRFGLIRMLAPEAIE
                     IFGAAKVAGLQTHQPQFEPGGVLRFWMSRAATEKTLGIR"
     gene            992..2455
                     /locus_tag="VFV87_00405"
     CDS             992..2455
                     /locus_tag="VFV87_00405"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5102239.1"
                     /translation="MRFATFQLRWVAARLAIMPDPYPINDFGPVMKGLAIGGLGIFHV
                     FLAQFAIGGGLLMCYFQWLAQTKGDKLARQFTDGFFKFLVLVSFVVGAITGVGMWFTS
                     IQVSARTIGLMVHHFHWIWAIEWTFFCLEIASGYLFYRYGTRLPDRVRMTLLVAYAVA
                     AWFSLFWINGILSWQLTPGAWEQSHSIWAGFFNPSFWPSLVFRTVVAMTIAALVACVV
                     ANFLPNLERSQREELVHKAARFLIPMVLMPFLAVWYFLAIPADSRSWVLGGSAAMSLF
                     FALGAGASLLVGAYALFGLVRQQLFVNGATALLLVSLAFAATAGGEFVREGVRKPFSI
                     RHLLYSNSIKEDEAARLRQRGCTTDDPYPLRNPEQYPDPQLAKGAQVYRNLCSVCHTV
                     HGTNALTDLTSTWSPDQMRINIAKLQRTKPFMPPFAGNAEEVEALVQFLRWESQGRPA
                     EWEATASNPAVIAQIDTWLAEAGTQSGLSLLDLRLKE"
     gene            2551..3615
                     /locus_tag="VFV87_00410"
     CDS             2551..3615
                     /locus_tag="VFV87_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102240.1"
                     /translation="MFPFDQPAPTAFYLTLYLGTFVLHVLPMAYVVAGSAVLLVRTLA
                     GTKPADDPLMDHLRQWLPFVLSAAITAGVAPLLFLQILYRREFYTANLLLFNRWMAIL
                     PVLIVAFYALYLTKGQWLWRQSWPLRAGVALAILACFLFIGWSWTENHLLSLAGLKVW
                     TENYSSGRIGHWSNEVLPRIGVWLGGVFAVMPALVAWPLARGAQSAGLTQRLALMALG
                     GLAVSLSSAVWYATVLDGPSRTAILGAACRAWQVAIALGIGLQVAGWIVAWRAGALAV
                     LARWLALIGVVLALIGGVATREALRTSRLDLAGLVPQHQAAANVGGFWLFALCLLVNA
                     AIIAGCVYLVARHVSPKVEN"
     gene            3752..4393
                     /locus_tag="VFV87_00415"
     CDS             3752..4393
                     /locus_tag="VFV87_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102241.1"
                     /translation="MFSYSEWHGQSSLLHKSGTVEFNTSYVTRCKPLWLACLKLLGVA
                     VMVAVGYGLLVPGLMHLQFSAWQAAAIASGLMMIYMGVAFFVRPEANTDNLGWGGGLA
                     NDPFQYSDNVNRFLWQAHCLLGPGRFTAETVLDFCVLVGLASREEVMDDHPEEAEEIA
                     PLDYATAMIAAAEERRAAPSLPAQPLPAAPAVAGQLDPNRFEGASGRRGQFGG"
     gene            complement(4423..4647)
                     /locus_tag="VFV87_00420"
     CDS             complement(4423..4647)
                     /locus_tag="VFV87_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102242.1"
                     /translation="MFRYSLRTLLMVLAVAPPVIAYCFLNRDGLALACGWSVIVGIFL
                     FWNWMVDKTASLGDQARGKPNHDPPKHFPP"
     gene            5700..>6293
                     /locus_tag="VFV87_00425"
     CDS             5700..>6293
                     /locus_tag="VFV87_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5102243.1"
                     /translation="MNGKDARISPRILSRWQAWLAALVGVAAVAAVVFAFRGSFGPQA
                     ASAQAPAVRPAAPTQARPAFGSPTARPASPATVAAPKGTRPAGSPAAPGASTQQVMAV
                     VNGEQISRTDLGRECIRRYGQEVLESMVNRQLIAAACAERKIVITEEDVNVEIDRIAA
                     RFSLPRDRWMQLLEEERGYSERQYKSEVVWPMLALRQL"
BASE COUNT         1054 a         1981 c         2067 g         1191 t
ORIGIN      
        1 atccgccaga cggccgagat tgcgatcgat caatgcttga tggtagagga gctgcggtgc
       61 gccgggatcc cggcgaagaa gcatttgcaa atggtcttgg gcttccttcg gctcccggtt
      121 cacgagcaat cgcaccagca tcaggcgtgc gccgtcgtgt tgcggatcga gggcgatcgc
      181 ctcccggcat agatcgcgag cctgcgcctg gtcctcgacc agcaggtaag tgttggctcg
      241 ccagatgagc ccctgcactc gatctgcacg actggtgcga tgccgttccc accagtctgc
      301 cagctccagc actcggtccg gctggagcgt ggccaagctc ccggcgattt gtgcttcagc
      361 gatctgcggc gcttcgggct gatccggatg ctcgcgccgg aagcgatcga gatcttcggt
      421 gccgcgaaag tcgccggcct gcagacgcac cagccgcagt tcgaaccggg cggcgtcctg
      481 cgtttttgga tgtcgcgagc agcgactgag aagacgctcg gcatccgcta actggccctc
      541 gcggcgggcg atccgggccg cgagcaagtc cagttctgcg ttatccggcc agagccaccg
      601 gcaacgttcc agcgcctggc gggcgtccgg caatttgcga tccgccaacg ctgccactgc
      661 agcttgccat tgccgatcac cccagatcca aaggccgatc cgatacgaag tgaaaccgat
      721 gatcgccagg aggacgagaa cccacagacg cttggaagcg actggtcgcg cgatgggctg
      781 gatgggcttg ttgcgacgtg acacagcaga aatcggagaa taccttccac gatagcgtcc
      841 gcggaaacat ccggcaactt gcaagtgggc ttcgagacca gacgccggaa agcaaatgcg
      901 accggctttc gaaccttcga accggtggac aggaatcggc gtgccgaccg tgttgtgaac
      961 tggagttgga tgttacgatt cggacctgca catgcgtttt gccacgttcc aattgcgctg
     1021 ggtcgcagcg cgcctcgcta taatgcccga cccctatccc atcaacgact tcggcccggt
     1081 gatgaagggg ctggccatcg gcgggctggg catctttcat gtgtttctcg ctcagttcgc
     1141 catcggcggc ggattgctga tgtgctattt ccagtggctg gcccaaacca aaggcgacaa
     1201 gctggcccgg cagttcaccg atggcttttt caagttcctg gtgctggtga gcttcgtggt
     1261 tggggcgatc accggcgtgg gcatgtggtt cacgtcgatc caggtgagcg cccggacgat
     1321 cggcctgatg gtgcaccatt tccactggat ctgggccatc gagtggacgt ttttctgcct
     1381 ggaaatcgcc tccgggtatc tcttctatcg ctacggcacg cggctgccgg accgcgtgcg
     1441 gatgacgctg ctggtcgcgt acgccgtcgc ggcgtggttc agcctgttct ggatcaacgg
     1501 catcctgtcc tggcagctca cgccgggagc atgggagcaa tcgcacagca tctgggccgg
     1561 gttcttcaat cccagcttct ggccgtcgct tgttttccgc acggtcgtgg ccatgacgat
     1621 cgcggccctg gtggcgtgcg tcgtggcaaa tttcctgccc aatcttgagc gatcgcagcg
     1681 cgaagaactc gttcacaaag cggcgcgatt cctcatcccg atggtcctga tgccgtttct
     1741 cgccgtctgg tacttcctgg ccatccccgc cgacagccgg tcctgggtcc tggggggcag
     1801 tgcggccatg tcattgttct ttgccctcgg ggccggggcg tcgctcctgg taggggcgta
     1861 tgcgctgttc ggcctcgtac ggcagcagct cttcgtgaat ggggcgacgg ccctcctcct
     1921 cgtttcgttg gccttcgcgg cgaccgccgg cggcgaattc gtccgcgaag gagtgcgaaa
     1981 accattttcg atccggcatt tgctgtattc gaactcgatc aaggaagacg aagcggccag
     2041 gctccgccag cggggttgca cgaccgatga tccttatccc ctccggaacc cggagcagta
     2101 tcccgatccg cagttggcca agggggcgca ggtctatcga aatctgtgca gcgtttgcca
     2161 caccgtgcac ggaaccaacg ccctgaccga cctgacgagc acctggtcac cggatcaaat
     2221 gcggatcaat attgccaagc tgcagcggac caagcccttc atgccgccgt ttgccggcaa
     2281 tgcggaggaa gtggaagccc tcgtacagtt cctccggtgg gagtcccagg gccgtccggc
     2341 cgaatgggag gccaccgcga gcaatcccgc ggtaattgcc cagatcgaca cctggctcgc
     2401 cgaagccggt acgcagtcag gcctgtcgct cctcgacctc cggctgaagg aatgatcgtc
     2461 gcacacctat ccgaagcgta agcgagggag tcaacaacac acctcactcc ctcgcttgcg
     2521 cgtcgggttg gtgtcattcg ctccgccaag atgtttccct tcgaccagcc cgcaccgacc
     2581 gcgttctacc tgacgctgta cctcggcacg ttcgtcctgc acgtgttgcc gatggcgtat
     2641 gtcgtcgccg gctcggcggt gctgctggtg cggactctgg cgggcaccaa gccggccgat
     2701 gacccgctga tggaccacct gcggcagtgg ctgccgttcg tcttgagcgc ggcaatcacc
     2761 gcgggggtcg cgccgctgct ctttttgcaa attctctacc ggcgcgagtt ctataccgcc
     2821 aatctgctgc tgttcaatcg ctggatggcg attctgccgg tgctgatcgt ggccttttat
     2881 gcgctctacc tgaccaaggg gcagtggctg tggcggcaga gctggccgct gcgggccggg
     2941 gtcgccctgg cgattctcgc ctgcttcctg tttatcggct ggtcgtggac cgagaaccac
     3001 ctgctcagcc tcgccggatt gaaggtctgg accgagaatt acagctccgg ccgcattggg
     3061 cactggtcga atgaagtgct gccgcggatc ggcgtctggc tcggcggcgt gttcgcggtg
     3121 atgcctgcgc tcgtggcctg gccgctggct cgcggggcgc agtccgccgg actgacgcag
     3181 agactagcgc tgatggcgct cggcggcctg gccgtcagcc tgagcagcgc cgtgtggtat
     3241 gccaccgtgc tcgatggccc gtcgcgaacc gcgattctcg gagcggcctg ccgagcctgg
     3301 caggtcgcga tcgcgctggg catcggcctg caagtcgccg gctggattgt cgcctggcgg
     3361 gctggtgccc tggctgtcct agcgcgctgg ctcgcactga tcggcgtggt gctcgccctc
     3421 atcggcggcg tcgccacgcg cgaagccctc cgcaccagcc gtctcgacct cgcgggcctg
     3481 gtgccgcaac atcaggccgc cgccaacgtt ggcgggttct ggctcttcgc cctgtgcttg
     3541 ctcgtcaacg ccgccatcat cgccggctgc gtctatctcg tcgcccgaca cgtgtcgccc
     3601 aaggttgaga attaatggtg cgttgaaagc gattttgcag cccattcggg ctaaattcac
     3661 gtcggacgca tgcaatgaat ttgcaatgtc cgctggccac cccggttccc gcccgccaca
     3721 ggaagctagg cttcccacgg ggagccgttg catgttttcg tactcggaat ggcacgggca
     3781 atcgagtttg ctccacaaga gcggcacggt ggagttcaac acgtcgtacg tcacgcgctg
     3841 caagcccctg tggctggcat gtctcaagct gctgggcgtg gcggtgatgg tggcggtggg
     3901 ctacggcctg ctggtgccgg ggctgatgca cctgcagttc tcggcctggc aagcggctgc
     3961 catcgcgtcg ggcctgatga tgatctatat gggcgtcgcg tttttcgtcc gcccggaagc
     4021 gaataccgac aacctgggct ggggcggcgg cctggcgaac gatccgtttc agtactcgga
     4081 caacgtcaac cgcttcctgt ggcaggccca ctgcctgctc ggccccggcc gcttcaccgc
     4141 cgagaccgtg ctcgatttct gcgtcctggt ggggctggcc agccgcgagg aagtcatgga
     4201 cgaccatccc gaagaggcgg aggagattgc cccgctcgac tatgccaccg ccatgatcgc
     4261 cgcagcagag gagcgccgcg ccgcgccgtc gctgccagcg cagccgctgc ccgcagcgcc
     4321 ggctgtggcg ggccagctcg accccaaccg cttcgagggg gcatctggca ggcgggggca
     4381 attcggcggg tgagcacccg gtcgtgttcg atcaagcgtt agtcacggcg ggaagtgctt
     4441 cggcgggtcg tggttcggct tcccccgcgc ctggtcgccg agcgacgcgg ttttatcgac
     4501 catccagttc cagaacagga aaatcccaac gatcacggac cagccgcacg ccagggccag
     4561 gccgtcgcga ttcagaaagc agtacgcgat caccggcggg gcgacggcca gcaccatcag
     4621 cagcgtgcgg aggctgtaac ggaacatggc ggcgcctagc tggccactgc aagggaaatc
     4681 aacaacgcga caacgaacag aaacggcccg aggacgagaa ctgttagcag caccggggaa
     4741 gtgcgaatca gcagcgtgcg gaggcggcta cgcatgggac tttttggggc gatacgccgc
     4801 cggctggagt gtcgccagct ctggtccccg ccgagcttgc tcgggggaac ccatgattgt
     4861 gcactataga gtgcagatca gcgtgctgtc gcccccaccg agcaagctcg atgggggcct
     4921 gaccgcacaa catcctgtaa gaaaagagtt gtggcactat tgacattagt gaacggattt
     4981 gtacaatttg ggatggcccg cacagcggcc cgttgcgcgc gagggcgcgg cgttgctctt
     5041 tgacaagttg gagatcggag gcacgacgca ccgcgagatg cttgcataga tgcgcaaggg
     5101 gcggcgcgcg ggaaatcttt ggcacctgcc gagcgagtag ttctcgggtg gggggaaatg
     5161 gcgaattgat tgacgtaacc cccgtgattc gctgtttttg tcaatttaat cgcaagggcc
     5221 gagggcgagg aactcggcga aacacggcga aaggtcgttc gggcggcgcg aatttcctga
     5281 cacgaggggg tacgccgtgt caatcaatgc gactcccctc gctcacgctg cgggccattt
     5341 cctcgctgac gctgcgggtt ggtgtgggcg actcggcgac cgtgaagagc gcaacagcgc
     5401 aacatcctca tgcggggggt gttgcgcggc gcaagcggtt ggaatgacag gagttaggga
     5461 aatgttgcgc ggggagcggc ggcctaaaaa aactgagaaa gttgcgcgct gcgctctggg
     5521 ggcttcggcg acgaagcacg gcttcgtcct acgtggccgt gctggcagcg ggggcgggcg
     5581 atttgccttg atcgctcggc ggagcttgcc tatagtccgc ggcccagaca gtttccgcgg
     5641 cagcggccta agtgcgccgg cgccgcattc ccgaatcatt ggacgacgga gcccggccca
     5701 tgaacggcaa ggatgcccgc atttcccctc ggattctcag ccgctggcaa gcctggctgg
     5761 cggccctcgt cggagtcgcc gcggtggcgg cggtggtgtt cgccttccgc ggcagcttcg
     5821 gcccgcaagc ggccagcgct caagcgccgg cggttcgccc ggctgctccc acgcaggccc
     5881 ggccagcgtt cggctcgccg acggctcgcc cggcatcgcc ggcaaccgtt gccgcgccca
     5941 agggaacgcg gccagccggt tcgcccgcag cgcccggcgc ctccacgcag caggtcatgg
     6001 cggtggtcaa tggcgagcag atcagccgca ccgacctcgg ccgcgagtgc atccgccgct
     6061 atggccagga agtgctggag agcatggtca accgtcagct catcgctgcc gcctgtgccg
     6121 aacggaagat tgtgatcacc gaagaagacg tgaacgtcga gatcgaccgg atcgccgccc
     6181 gcttcagcct gccgcgcgac cgctggatgc agcttctgga agaagaacgc ggctatagtg
     6241 agcgccaata caagagcgaa gtcgtctggc cgatgctcgc cctgcggcag ctt
//
LOCUS       DASUGB010000022         8480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1531052, whole genome shotgun sequence.
ACCESSION   DASUGB010000022 DASUGB010000000
VERSION     DASUGB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531052"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFV87_00430"
     CDS             complement(<1..1037)
                     /locus_tag="VFV87_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5102244.1"
                     /translation="MPGERLTNFGGNLTIEPQAVFTPGSEEEVLQILRDERGKRIRAI
                     GRLHSWSSAPLAEEVLLDLRKLKSLEVTSDKQGPLARIGAGCQIKDVLRELDRHGLTL
                     PTIGLISEQTIAGAAATGTHGSGRASISHYIAAVRLACYDPAMAEPVVREVRSGPELQ
                     AARCSLGLMGIVLSVEVRPRPQYRIEEWFALHNSLEEVLAAEAEAPLQQFYLIPWQGK
                     YMGQHRRETEQPRSWLAWLYRFYWFLTIDVGLHLAMLLLVRVLGSRWAIKTFFRTVIN
                     LTVIRGWHVVDRSPAMLIMEHELFHHIEIELFIKQSRLAEAMDFVQEAIQCFDGDSAA
                     FPPHWRQRLEA"
     gene            complement(1064..2851)
                     /locus_tag="VFV87_00435"
     CDS             complement(1064..2851)
                     /locus_tag="VFV87_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX5102245.1"
                     /translation="MAALLVAPAASFAQDVVQPKIGETIAKLRFKDIRYLPRSLEDFG
                     EKKAFVLVFTNTTCPLVQRYWPKLKRLDEQYRDQGVQFVSVNVGADDEIQEIAQQAID
                     FGIEFPLVKDVDGSCVKALGALRTPEVAVLDDKFRLVYRGRIDDQVRLVGSRPDVQND
                     DLANAIEAVLAGRAPKAAETPVDGCLITLPSPREVSTPVTFHEHIAPLFQKHCQECHH
                     TGGGAPFSLVTLEEVSSQAQMIREVVLDRRMPPWYAHRRQSFVNERGLSAKERTQIAD
                     WVQVKMPAGDPAKTPPPRTFADSKWEIGEPDLTITALEAHELPADGFVDYRYAILPHV
                     FLHDTWISAAEILPSNPAVVHHCNMAFVTLGQSFSDGNFITGRVPGGTAMTLNDGVAL
                     RIPAGSVVGLQIHYTTTGKPEKNRMSIGFRYPREVVHKELHHLQVSTSRFEIPPGAAA
                     HKVSATRTLPCDALGVGMFSHMHLRGKDMTFIAHSPTGEDETLLSIPNYHYGWQQNYR
                     WQPGTRRFAKGTKIEVIAHYDNSAFNPFNPDPTAAVKHGPQTVEEMMYGFFFYTDASE
                     DLKLNVDPKTGRVVKSEADQPAKAQDQGP"
     gene            complement(3059..3511)
                     /locus_tag="VFV87_00440"
     CDS             complement(3059..3511)
                     /locus_tag="VFV87_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5102246.1"
                     /translation="MDKNKVVEKLNECLRHEWTGVAQYAQAGFVVAGLWREVYSDMFF
                     DNAKESFGHAKQVGEKIVALGGLPTVERNPVRQTQDLAELLNFALEFESKAVKLYNEA
                     LALAAGDRALEVFIENILEEEQAGVDELTRILRDPKAIAASGGQAKVG"
     gene            4026..4445
                     /locus_tag="VFV87_00445"
     CDS             4026..4445
                     /locus_tag="VFV87_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5102247.1"
                     /translation="MKLSRTVSYAVRATLQLAQLEAEGPVPCSRLATEGKMPERFLLQ
                     ILRNLVTHGLLRSTRGVDGGYSLVKPAAEISLLEVIEAIEGPYDSTLEVGEGLPQDSQ
                     TKLRRALEDVTTTTRGQLEAIKLSALLKPPSGEHRPK"
     gene            4518..5339
                     /gene="hisN"
                     /locus_tag="VFV87_00450"
     CDS             4518..5339
                     /gene="hisN"
                     /locus_tag="VFV87_00450"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX5102248.1"
                     /translation="MTEIPASLAAEVAPRLDVVLSAGKEAGRLTLQYFQQGNFQVERK
                     SDASPVTIADRAAEQLLRERIAAAFPRDGIIGEEFGTSEGTSGFNWILDPIDGTKSFI
                     HGVPLYGTLIGVEHEGRCLAGLIFMPALDEGVYASAGQGAWHFRGASQPTPARVSTKA
                     KLSDGLFLTSAVNTFAERGALAAFEALQHAAYITRTWGDCYGYLLVATGRAEAMVDPI
                     MNLWDIAPLQPILEEAGGTLTDWQGNATIHGGEAIGTNGLVLEEVLAATRRNSKG"
     gene            5343..5894
                     /locus_tag="VFV87_00455"
     CDS             5343..5894
                     /locus_tag="VFV87_00455"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5102249.1"
                     /translation="MPDVNLVSRLTEFVSRENHLDLPPIATPEQVHQTERSIGFALPP
                     LLRSCYLEIANGGFGQGYGIIGVKGGHESDFGDVVSTYQELKSCVEYFGNKWLPGLLP
                     FCAWGCNIFSCVSCHDERLPIFTFEEGKVWHEDYNLNDFFEMWMQGEDILAHGGRYEI
                     EEVMKTNPFTGAQMTVRRKKRVR"
     gene            complement(5901..7232)
                     /locus_tag="VFV87_00460"
     CDS             complement(5901..7232)
                     /locus_tag="VFV87_00460"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5102250.1"
                     /translation="MFSQLALVVTLAVSADPATQLKYVGTMTPVKEDGNPAVKRFTLT
                     VQPLAGEEGQLAWTLEETGRGGWSWLDHFGRWAVTRSVSEGAPDAGVGPALLYEREDG
                     KSLVPILSPHFADGAKLEPGATWDEGRLEYKVLGEEQRAGLPCWEIEVRSPYGHKRTL
                     LVEKASGTLVVAVRETVFIGQGQEHKLNFELTEAKELTEADAASTAAALEQWLKLRDT
                     LAWTPRGGRTELSTEQIATLKAELPKLLETSAAGPLATIAAAAEADAKGQRNRAGAVA
                     ALREAALGQPLGKFALQDIAGKPLTEEALAGRVVVLHFWEYRDTPLEEPYGQTGYLDY
                     LSRRRGEAGVLVFGVTVADNIDDEQARRTAASSARKLKAFMNLSYPILLDDGTLLKRL
                     GDPRQASGKLPLFVVIGKDGKLAEYHAGLYDVKTNEGLAELDAVIGKALAK"
     gene            complement(7399..7920)
                     /locus_tag="VFV87_00465"
     CDS             complement(7399..7920)
                     /locus_tag="VFV87_00465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5102251.1"
                     /translation="MTNIRPALASDELTIQAVHRHAFPTDAEARLVALLAARGKAAVS
                     LAAEADGQIVGHMLFSPATIETPGLPPLVGLGLAPVAVLPEYQNRGIGTALVETGLEA
                     ARQLQVPFVVLIGHPAFYPRFGFMPAASFGLTCDFGSGAEFQIIDLLEGSLPAAGGVV
                     RYAEEFYELFGPP"
     gene            8229..>8480
                     /gene="rpsO"
                     /locus_tag="VFV87_00470"
     CDS             8229..>8480
                     /gene="rpsO"
                     /locus_tag="VFV87_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5102252.1"
                     /translation="MTVTKERKRELIEQYRREDADSGSPEVQIAVLTARINNLTEHMR
                     THDKDYASRRGLLALVSRRRTLLDYLRRKNAPAYLGIINR"
BASE COUNT         1565 a         2664 c         2674 g         1577 t
ORIGIN      
        1 cacgcttcca agcgctgacg ccaatgcggc ggaaaggcgg cggaatcccc atcgaagcac
       61 tggattgctt cctgcacgaa atccatcgct tccgccaggc gcgattgctt gatgaacagc
      121 tcgatttcga tgtggtgaaa cagctcgtgc tccatgatca gcatggccgg cgagcgatcg
      181 acgacgtgcc agccgcggat caccgtcagg ttgatcaccg tgcgaaaaaa ggtcttgatg
      241 gcccagcggc tcccaagcac gcgcaccagc agcagcatgg ccaggtgcag gcccacgtcg
      301 atcgtgagga accaataaaa gcggtagagc caggcgagcc agcttcgcgg ctgctcggtc
      361 tcccggcgat gctggcccat gtacttccct tgccagggga tgagatagaa ctgctgcagc
      421 ggggcttccg cttcggcagc gagcacttct tcgagcgaat tgtgcagagc aaaccattct
      481 tcaatgcggt actgcggcct cggccggact tcgaccgata ggacaattcc catcaggccc
      541 agcgagcagc gcgccgcctg gagttccggg cccgaacgga cctcgcggac gaccggctcg
      601 gccatcgcag ggtcatagca ggccaggcgg acagccgcga tgtaatgcga aatgctggcc
      661 ctgcccgagc cgtgcgttcc ggtggcggca gcgccggcga tcgtctgttc ggagatcagg
      721 ccgatcgtgg gcagcgtcag tccgtgccga tcgagctcgc ggagcacgtc tttgatctga
      781 caaccggcac cgatcctggc cagcgggcct tgtttatcgg atgtaacctc cagcgacttc
      841 agctttcgca gatcgagaag cacctcctcg gccagcggcg cgctgctcca cgagtgcagc
      901 cggccgatcg cccgaattcg cttgccgcgc tcgtcgcgca ggatttgcag cacttcctct
      961 tcgctgcccg gcgtgaaaac ggcttgcggc tcgattgtca gattgccgcc gaagttggtc
     1021 agccgctcgc cgggcatcgc ttgattcgct cgatccaaat tccctacggg ccttggtcct
     1081 gagctttcgc cggctggtca gcttccgact tgaccacgcg ccccgttttc ggatcgacat
     1141 tcagcttcag atcctcgctc gcgtccgtgt aaaagaagaa cccgtacatc atctcctcga
     1201 cggtctgcgg gccatgcttc acggccgccg tggggtctgg gttgaatggg ttaaaggccg
     1261 aattgtcata gtgggcgatc acttcgatct tcgtcccttt cgcaaatcgc ctggttcccg
     1321 gctgccagcg atagttctgc tgccagccgt aatggtagtt aggaatcgac agcagcgttt
     1381 cgtcctcgcc cgtcggcgag tgggcgataa aggtcatgtc tttgccgcgc aagtgcatgt
     1441 gcgaaaacat gccgacgccg agcgcatcgc aggggagcgt gcgggtggca gagaccttgt
     1501 gggcagccgc gcccggcgga atctcgaatc gagaggtgct cacctgcaaa tggtgcaatt
     1561 ccttgtgcac gacttcgcgt ggataacgaa agccgatcga catgcggttc ttttcaggct
     1621 tgccggtcgt cgtgtagtga atttgcaggc cgacgaccga accggcggga atgcggagcg
     1681 ccacgccatc gttcagcgtc attgccgtgc cgcccggcac gcggcccgtg atgaaattgc
     1741 cgtcgctgaa gctctggccc agcgtcacga aggccatgtt gcaatgatgg acgaccgccg
     1801 ggttggaagg cagaatctcg gccgcgctga tccacgtgtc gtgcagaaaa acatgcggga
     1861 ggatcgcata ccgatagtcc acaaagccgt cggcaggcag ctcgtgggct tccagggccg
     1921 tgatcgtcag atcgggctcg ccgatctccc acttggaatc ggcaaacgtg cgcggcggcg
     1981 gtgttttggc gggatcgccc gccggcatct tgacctggac ccaatcggca atctgggtcc
     2041 gttctttcgc cgatagccct cgctcgttga caaacgactg ccggcgatgg gcgtaccaag
     2101 ggggcatccg ccggtcgagg acgacttcgc gaatcatctg tgcctggctg ctgacctcct
     2161 ccagcgtcac gagcgagaac ggagcgccac cccccgtgtg atggcactcc tgacagtgct
     2221 tttggaagag cggcgcgatg tgctcgtgaa aggtgaccgg cgtgcttact tcacgcggcg
     2281 atggaagcgt gatcaggcag ccgtccaccg gcgtttcggc cgctttcggc gcacgccccg
     2341 ccagcaccgc ctcgatggca tttgccagat cgtcgttttg aacatccggc cgggaaccga
     2401 ccagccttac ctggtcgtca atccgtccgc gatagaccag ccggaatttg tcatccagca
     2461 cggcgacttc gggcgtccgc agtgcgccga gcgccttgac gcacgagccg tccacgtcct
     2521 tgaccagcgg aaactcgatg ccgaaatcaa tggcctgttg ggcaatctcc tggatctcgt
     2581 cgtccgcgcc cacattgacg ctgacgaact gcacgccctg gtcgcgatac tgctcatcca
     2641 gccgcttgag cttgggccaa taccgttgga cgagcggaca ggtcgtattc gtgaagacga
     2701 gcacgaaggc cttcttctcg ccgaaatctt cgagcgaccg cggcaagtag cgaatgtcct
     2761 tgaaccgcag cttggcgatc gtttcgccga ttttaggctg cacgacatcc tgcgcgaacg
     2821 aagcagccgg cgcgacgagg agcgctgcca cgaaagtcac tcgccacagc atgcttgtcg
     2881 cctcgcgatc ttgcggagct gccatatcgt tgctgcccag atcgtaccac tccagtgcca
     2941 cctcctacaa atcagtgcct gtgccgcctc caacggcgtt tcgccgtcgc gcagccagtg
     3001 cggcggtggc gtcttgcgct ggtcgttcga tcaacttttg cggaggagtg gacctcgctc
     3061 agcccacctt ggcctgtccg ccgctggcgg cgatggcctt ggggtcgcgg agaatgcggg
     3121 tcagctcatc gacgccggcc tgctcttctt cgaggatatt ttcgataaag acttcaaggg
     3181 cccggtcgcc agcagccagc gccagcgcct cgttgtagag cttgaccgcc ttggactcaa
     3241 attcaagagc gaaattgagc aactccgcga ggtcctgggt ctgcctgacc gggttgcgtt
     3301 ccaccgtcgg cagaccgccc aaggcgacga ttttctcgcc cacctgcttg gcgtggccga
     3361 acgattcctt ggcattatcg aagaacatgt cggaatagac ctcgcgccac aagcccgcga
     3421 cgacgaagcc agcctgtgca tattgcgcga cgcccgtcca ttcatgccgg agacactcgt
     3481 tgagcttttc gacgactttg ttcttatcca tgacgcaaaa cctcccaggt gaaatgtgtg
     3541 taagtgtcga tgtttggcca gtaaacgcaa ctcctcaacg cccgtatctt agcgggccgc
     3601 ggtggctggt ccagagcgaa ctaactcttt ttgtggaaat ggcttatgac gcgcctctaa
     3661 cgagaggaag tctcgccctg acggagcggg caattgagaa cggctttcaa gtccctcggc
     3721 cggagagcac ccaaaaagtg gtgcaaagcc cacgccaaaa tcccccggaa ttcgattctt
     3781 ctgagcggaa cccgattgat tgacatgcgc gcgtggttcg gtacattggg cgctcggctg
     3841 aacctccgca aagtgaatcg accatctcgg cgttctgctt ggggaagtcg cagccgccgc
     3901 cagtgccggc cggcgagccg cgctggcagc gccctggacg gaagagggtt cgtagggcaa
     3961 ggcgacatgc caattgcgcg tgtcgcttgg gcgcagtgac aattcaccat gatggaacgt
     4021 gccccatgaa actctcgcgt accgtctcgt atgccgtgcg ggcaaccctg cagcttgccc
     4081 agcttgaagc cgaggggccg gtcccgtgca gccgcctggc caccgaaggc aaaatgcccg
     4141 agcggtttct cttgcagatt ctgcgtaacc tggtgacgca cggcctcttg cgctccaccc
     4201 gcggcgtcga tggcggctat tcgctggtga agccggccgc ggagatttcg cttttagaag
     4261 tgatcgaggc catcgagggg ccatacgatt caaccctgga agtgggcgaa gggctgccgc
     4321 aggattcaca aaccaagctt cgccgagccc tggaagacgt gaccacgacg acccgcggcc
     4381 aactcgaagc gatcaagctc tccgccctgc tcaagccgcc cagcggcgag cacaggccca
     4441 agtgagatag taacctgaca tgtaggcgag ggagtttcgg ctggcaaggt cgcaatcgct
     4501 tcattagtct aggcggaatg actgagattc ccgcctccct tgccgctgaa gtcgccccgc
     4561 gcttggacgt tgtcctttcc gccggcaaag aagcgggccg cctcacgctg cagtacttcc
     4621 agcagggcaa ctttcaggtc gagcggaaga gcgacgcctc gcccgtgacg atcgccgacc
     4681 gcgcggccga gcagctcctt cgcgagcgga tcgcggctgc tttcccccgc gatggcatca
     4741 tcggcgagga attcggcacg agtgaaggga cttccggctt caactggatc ctcgacccga
     4801 ttgacggcac caagtcgttc atccacggcg taccgctcta tggcacgctg atcggcgtcg
     4861 agcacgaagg ccgctgcctc gccgggctga tcttcatgcc ggcgctggat gaaggcgttt
     4921 atgcctcggc agggcagggg gcctggcatt tccgcggcgc ctcgcagccg acgcccgcgc
     4981 gggtctcgac caaggcgaag ctgagcgacg ggctgttcct cacctccgcc gtcaacacct
     5041 tcgccgagcg cggcgcattg gccgcattcg aagccctgca acacgctgcc tacatcaccc
     5101 gcacctgggg cgattgctac ggctacctgc tggtcgcgac cggccgggcc gaagcgatgg
     5161 tcgatccaat catgaacctc tgggacatcg cccccctgca gccgatcctc gaagaagccg
     5221 gcggcacgct gaccgactgg caaggcaacg ccacgatcca cggcggcgaa gcaatcggga
     5281 cgaatgggct ggtgctggag gaggtgctgg ccgccacacg ccgaaattcc aaggggtgag
     5341 cgatgcccga cgtcaatctt gtcagtcgcc ttacagagtt cgtgtcacgg gaaaaccact
     5401 tggaccttcc gccgatcgcc acgcctgagc aggttcatca aacagaacgg tcaattggtt
     5461 ttgcgttgcc gccgttgctt aggtcttgct atctcgaaat cgctaacggg ggattcgggc
     5521 agggctatgg gatcattggg gtcaagggcg gacacgagtc ggactttggg gacgtcgtga
     5581 gcacgtatca agagcttaag agctgcgttg aatacttcgg caacaaatgg ctcccaggct
     5641 tgctgccctt ctgcgcatgg ggctgcaaca tcttttcgtg cgtgagctgt cacgacgagc
     5701 gacttccgat tttcaccttc gaggagggaa aggtgtggca tgaagattac aacctcaacg
     5761 acttttttga gatgtggatg cagggcgaag acattctagc gcacggcggt cgatacgaga
     5821 tcgaagaagt catgaagacg aatccattca caggcgcaca gatgacggtc cgccgcaaga
     5881 agcgagtccg ctaggtcgct tcatttggcc agcgccttcc caatcaccgc gtccaactcc
     5941 gccagccctt cgttcgtctt cacgtcatag aggccggcgt ggtactcggc gagcttgccg
     6001 tccttgccaa tgacgacgaa cagcggcagc ttgccgctgg cctggcgggg atcgccgagg
     6061 cgcttgagca gcgtgccgtc gtccagcagg atcgggtagc tgaggttcat gaaggccttg
     6121 agcttgcgag cggaggacgc ggcggtgcgg cgggcctgct cgtcgtcgat gttgtcggcg
     6181 accgtgacgc cgaagacgag cacgcccgcc tcgccgcggc ggcgggagag atagtcgaga
     6241 tagccggtct ggccgtaggg ctcttccagc ggcgtgtcgc ggtattccca gaagtgcagc
     6301 acaacgaccc tgcccgccag cgcctcctcg gtgagcggct ttccggcgat gtcttgcagg
     6361 gcgaacttgc ccagcggctg accgagagcg gcctcacgaa gggcggcgac cgcgccggcc
     6421 cggttccgct gccccttggc atcggcttcg gcagcggcgg cgatcgtcgc gagcgggccg
     6481 gcggcggaag tctcgagcag cttcggcagt tcggctttga gcgtggcgat ctgctcggtc
     6541 gaaagctcgg tccggccgcc gcgcggcgtc caggcgagcg tgtcgcgaag cttgagccac
     6601 tgctcgaggg ccgcggctgt gcttgcggcg tcggcttccg tcagctcctt ggcctcggtc
     6661 agctcgaagt tcagcttgtg ctcctggccc tggccgatga agaccgtctc acgcacggcg
     6721 acgacgagcg tgccgctggc tttttcgacg aggagcgtcc gcttgtggcc gtaggggctg
     6781 cggacctcga tttcccagca gggcaggccg gctcgctgct cttcgccaag gactttgtat
     6841 tccaggcggc cctcgtccca ggtcgccccc ggttcgagct tcgccccgtc ggcgaaatgg
     6901 ggtgaaagga tcggcaccaa gcttttgccg tcttcgcgtt cgtagaggag ggcgggaccg
     6961 acgccggcat ccggcgctcc ctcgcttacg ctgcgggtta cagcccagcg gccgaaatgg
     7021 tcgagccacg accagccgcc gcggccggtt tcttccagtg tccaggcgag ttggccctcc
     7081 tccccggcca gcggctgcac ggtcagcgtg aaccgcttga ccgccggatt gccgtcctcc
     7141 ttcacgggcg tcatcgtgcc gacgtatttg agctgcgttg ctggatcggc cgacaccgcg
     7201 agcgtaacaa ccagcgcgag ttgcgagaac attcggacct cagacgagtg gagagcgatc
     7261 ctgcagatcg ccaatggggc gggggacagc cggatggctg accggcccat cataaccgca
     7321 gccgcccgga agggcgagag aatcaacgtc gtcgttcgct ccgcgaacga acgcgcccta
     7381 ccgcggggcg agaggcggct atggcggacc aaacagctcg taaaactcct cggcatagcg
     7441 gacgacaccg ccggctgcgg gcaaagagcc ttcgagcaga tcgatgatct ggaactcggc
     7501 gccgctgccg aagtcgcaag tcaggccgaa gctggcggcg ggcatgaagc cgaagcgggg
     7561 gtagaacgcg ggatggccga tcaggacgac gaagggaacc tgaagctggc gcgccgcttc
     7621 caggccggtt tcaaccaagg ccgtgccgat gccgcggttc tggtactccg gcagcacggc
     7681 caccggcgcg aggcccaggc caacgagcgg cggcaggccc ggcgtttcaa tcgtcgccgg
     7741 gctgaagagc atgtgcccca cgatctgccc gtccgcctcc gccgccagcg ataccgccgc
     7801 cttcccgcga gcggctaaca gggcaaccag ccgagcctcg gcatcggttg gaaacgcgtg
     7861 tcgatgcacc gcctgaatcg tcagctcgtc gcttgcgagt gcggggcgaa tgtttgtcat
     7921 gggatggcca ttctatagct gcggagcagc gccagttcta agccagccgc ttgcccacgc
     7981 aaaggcaatc gccgcaagaa catacgcgat ttgcggtcca tcgccggccg cggccgggca
     8041 gccccgcttg ttgcccttgc aatccctcgg aggactgata gattaactgg cttgtcgttt
     8101 ggtttagttg gctgggacaa ttgtcccgta cgtgcagtcg tggaggagat tcgtcgcacc
     8161 tgaatggtcg gcccggagca agcttgcccg gcgacagcgt aggcagcgga ggagtcacgc
     8221 cagaagcaat gacagtcacc aaagagcgga agcgggaatt gatcgagcag tacaggcggg
     8281 aggacgcgga ctccggatcg ccggaggtcc agattgcggt cctcacagct cggattaaca
     8341 acctgaccga gcacatgcgg acgcatgaca aagactatgc cagccggcgc ggcttgttgg
     8401 cattggtcag ccgccggcgt acgctgctgg actacctgcg ccggaagaat gcccctgctt
     8461 acctcggcat catcaaccga
//
LOCUS       DASUGB010000023         5351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1253187, whole genome shotgun sequence.
ACCESSION   DASUGB010000023 DASUGB010000000
VERSION     DASUGB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253187"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VFV87_00475"
     CDS             <1..1175
                     /locus_tag="VFV87_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5102253.1"
                     /translation="IVRGTLAACGRDVLDAGVAATPTLGVLVRGQNAAGGVQISASHN
                     PPPYNGIKLFNRDGRVLPARDGEAVLRAYRQPVPAWKAFDRIGQISTLQDTTNNHLSC
                     VLGTINVDQVSKQKFRVLLDSNAGAGSVLGRRLLTDLSCEVTVLGGQPNGKFEHAPEP
                     TAPNLAAVCSRVKESGAAVGFCQDPDADRLAIIDENGRYIGEEYTLAICLNHELRERL
                     GPIVTNCATSRMAEDLAAKYGVPFYRSAVGEANVVDKMLDVGAVFGGEGNGGPIDPRV
                     GLVRDSFVGMALVLAAMASRGKRVSELADELPRYEIVKRTASLPANKLPAALDAAQRR
                     FADAKPNRLDGLRLDWADRWLIVRGSNTEPIVRIIAEAPTAAAAEELVATCANVIA"
     gene            1194..2333
                     /locus_tag="VFV87_00480"
     CDS             1194..2333
                     /locus_tag="VFV87_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5102254.1"
                     /translation="MRRIYLDYNATTPIAPSVREAMLPFLGEHFGNPSSSHALGRACL
                     EAVEDARGQVASLLGADAEEIVFTGCGSESNNLALKGLLLRGGRSGGGHLVISAIEHP
                     SVVETARFLERLGCDVSIVPVTGQGVVQPEAVRKALRDDTVLVSIMHANNEIGTIQPL
                     VSIAETCHEAGVLLHTDAAQSAGKIRTQVDELQVDLLTIAGHKLYAPKGVGALFVRQG
                     IDLEPLVHGGGQESGLRSGTENVASIIGLGAAASLAAKSLDAAQERLAQLRDRLLAAL
                     REGIGEGLIVHGQHAPRLPNTLAVSFPAASGYDLLARVPELCASTGSACHSGAQHISP
                     TLAALGASADAARGTIRLSLGWYTSEEEIERAASLLIGAWEAVRE"
     gene            2381..3490
                     /locus_tag="VFV87_00485"
     CDS             2381..3490
                     /locus_tag="VFV87_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5102255.1"
                     /translation="MKAISRRTMIQVTAAAAAGTGLAPFAANAAEAEKDVPWLAEIQT
                     PPEKLRSDAPKLTELLRDERGRPITTVEGWKARREDLLRWWLDFLGPLPAERKEPPKL
                     EVLAEDRPEGVIRQLVRYEIEPGIVTEAYLLQPGKLNGKAPGVAVFHSTVDHSIRQPS
                     GVEGAPEKAFGLKLAQKGCVAFCPRNYLWPDNGRIAAQAEAKAFAERRPKSKGMAKML
                     YDALVAVDILAALPKVDAERLGAAGHSLGAKETLYLAAFDERIKATVSSEGGIGTKFS
                     NWNAPWYLGPAIDEPAFKHEHHELLALTAPRPFLLVGGESADGDRGWPFIAAALPVYE
                     LHGKPARLGQFNHRKGHAVPPEAERRIEEWLLTYL"
     gene            complement(3563..3937)
                     /locus_tag="VFV87_00490"
     CDS             complement(3563..3937)
                     /locus_tag="VFV87_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102256.1"
                     /translation="MTSSSAHRPLLQFSLQSLLIVTAMVAITLAFDRLVGYKAAVHYC
                     LLVFAVGPWFAYLFSECLPIRATQLRTAAANLMLLVLFIATLKLAEMVLTGPVVILVA
                     LAALLLWTPQYMFFFVWRTDEV"
     gene            complement(3964..4746)
                     /locus_tag="VFV87_00495"
     CDS             complement(3964..4746)
                     /locus_tag="VFV87_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5102257.1"
                     /translation="MFEAYWGLQRGPFSDDAARRSLEGSPTHAEALARLEFLIESRSR
                     FGLLLGPAGSGKSLVLGELARRAERTGSAVTLVAAAGSDAKGLLAGLAMGLGCPPEST
                     ICPWAAVTDRLEELKLEGLGATVLFDNLDRATGEAQAKVEALLGIADSPLTVIATARV
                     ESAARLTRGLVDQVSLRIDLTPWNEDETREYLLASLAQAGRKQPAFNDAAVRRLFELS
                     GGAPRKVNQLAELALLAGASHNLVQVDAETIDAVEEELGVVR"
     gene            4957..>5351
                     /locus_tag="VFV87_00500"
     CDS             4957..>5351
                     /locus_tag="VFV87_00500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX5102258.1"
                     /translation="MSERFYVEPPIDLPHVTLTGDEFRHLAAVMRARPGDEVTLFDGS
                     GAEFAARIVHIGKREARLEIIERREVSRELPLELTLAVALPKGERQKWLVEKLTELGV
                     TRLVPLVTERGVAQPTIGAVDRLRRSVIEA"
BASE COUNT          925 a         1666 c         1884 g          876 t
ORIGIN      
        1 agatagtgcg cggcacgctg gcggcctgcg gtcgggacgt gctcgatgcg ggagtcgcgg
       61 cgacgcccac gctgggagtg ctcgttcgcg gccagaacgc tgccggcggc gtgcagattt
      121 cggccagcca taatccgccg ccgtacaacg gcatcaagct ctttaaccgc gacgggcggg
      181 tgctgcctgc gagggacggc gaggcggtac tgcgcgccta tcggcagcca gtgccggctt
      241 ggaaggcgtt cgatcgaatc ggtcagattt caacgcttca agacacgacc aacaatcacc
      301 ttagctgcgt actgggcacg atcaacgttg atcaggtttc caagcaaaaa ttccgcgtct
      361 tgctcgacag caacgccggt gcgggaagtg tgctgggcag gcgcttgctg acggatttga
      421 gctgtgaggt cacggttctg ggcgggcagc cgaatgggaa attcgagcac gcgcctgagc
      481 cgacagcacc taatctggcg gccgtttgca gtcgcgtaaa agagagcggc gccgccgtcg
      541 gcttttgcca ggatccggat gcggatcggc tggcgattat tgatgagaat gggcggtata
      601 ttggcgaaga gtacacgctg gcgatttgcc tgaatcacga gctgcgcgag cggctggggc
      661 cgattgtgac gaactgcgcc accagccgca tggcggagga ccttgcggcg aagtacggcg
      721 tgccgttcta ccggtcggcg gtgggcgagg caaatgtcgt cgataagatg ttggacgttg
      781 gggcggtctt tggcggcgaa gggaacggcg ggccgatcga cccgcgggtc ggcctggtgc
      841 gggacagctt cgtggggatg gccctggtgc tggccgcgat ggccagccgc ggcaagcgcg
      901 tcagcgagct ggccgatgag ctgccgcgat acgagattgt caagcggacg gcttcgctgc
      961 ctgccaacaa gcttcccgcc gccctcgatg cggctcagcg gcgcttcgcg gacgcgaagc
     1021 ccaaccggct cgacggcttg cggctggatt gggccgatcg ctggctgatc gtccgcggca
     1081 gcaataccga gccgatcgtg cggatcatcg ccgaggcgcc gacggctgcc gcggcggagg
     1141 aattggtggc gacgtgcgcg aatgtgatcg cgtaacgtcg gacggaggca ggtgtgcgac
     1201 ggatttattt ggactacaac gcgacgacgc cgatcgcgcc gagcgtgcgc gaggcgatgc
     1261 tgccgttcct gggtgagcac ttcggcaatc cgtcgagcag ccatgccctg ggccgcgctt
     1321 gcctggaagc ggtcgaggac gcccgcggcc aggtggcgag cctcctgggc gccgatgccg
     1381 aagagatcgt gttcaccggc tgcgggagcg agagcaacaa cctggctctc aagggcctgc
     1441 tgctgagggg cggccgttcc ggcggcgggc acctggtgat ctcggcgatc gagcatccca
     1501 gcgtcgtcga aacggcccgg tttctggagc gactgggctg cgacgtgagc atcgtgccgg
     1561 tgacgggcca gggcgtcgta cagccggagg cggtccgcaa ggcgctgcgc gacgacaccg
     1621 tgctggtcag catcatgcac gccaacaacg aaatcgggac gatccagccg ctcgtgtcga
     1681 tcgccgaaac gtgccacgaa gcgggtgtgc tcctgcacac cgacgccgcg caaagtgcag
     1741 gcaagatccg cacgcaggtc gacgagctgc aggtcgatct gctgacgatc gccggacaca
     1801 agctttatgc tccgaaaggc gtcggcgcgc tgttcgtgcg ccaggggatc gacctggagc
     1861 cgctcgttca cggcggcggc caggaatcgg gcctgcgttc ggggaccgaa aacgtcgcca
     1921 gcattatcgg cctcggcgct gcggcgtcgc tggccgccaa gagcctcgat gccgcgcagg
     1981 agcggctggc ccagcttcgc gatcggctgc tggcggcctt gcgcgaggga attggcgagg
     2041 ggctgatcgt gcacgggcag cacgcgccgc gcttgccgaa tacgctggcg gtcagctttc
     2101 ccgccgccag cggttacgac ctgctggctc gggttccgga gctgtgtgcc tcgaccgggt
     2161 cggcctgcca cagcggcgcg cagcatattt cgccgacgtt ggcagccctc ggcgctagcg
     2221 ccgacgccgc ccgcggcacc atccgcctca gcctgggctg gtacacgagc gaggaggaaa
     2281 ttgagcgggc cgccagtttg ctgatcgggg cgtgggaggc ggtgcgggag tagggccgga
     2341 gagggtcggg gttcggggtt cggggttcag gaggcgtggt atgaaggcga tcagtcgtcg
     2401 gacgatgatt caggtgacgg cggcggcagc ggcggggact gggttggccc ccttcgccgc
     2461 aaacgccgcc gaggcggaga aggacgtccc gtggctggcc gagattcaaa ctccgccgga
     2521 aaagctacgg agcgatgctc ccaaactgac tgagctgctg cgggacgaac gcggccggcc
     2581 gatcaccacg gtcgagggct ggaaagcccg ccgcgaggat ttgctccgct ggtggctcga
     2641 ttttctcggc ccgctgccgg ctgaacgcaa ggaaccgccc aagctggaag tcctggccga
     2701 ggaccgcccc gaaggcgtca tccggcagct tgtgcggtac gagattgagc cggggatcgt
     2761 gacggaggcc tatctgctgc agccgggcaa actcaatggc aaggcgccgg gcgtcgccgt
     2821 ctttcattcg accgtcgatc actccatccg ccagccgtcc ggcgtggaag gagcgcccga
     2881 gaaggcgttt ggcctcaagc tcgcgcagaa ggggtgcgtc gccttctgcc cgcggaacta
     2941 tctctggccg gacaacgggc ggatcgccgc acaggccgag gccaaggcct ttgccgagcg
     3001 ccggccgaag tccaaaggga tggcgaagat gctctacgat gcgctggtcg cggtcgatat
     3061 cctcgcggcc ctgcccaagg tcgatgccga gcggctcggc gcggcgggcc attcgctggg
     3121 cgcgaaagaa acgctgtatc tggccgcgtt tgacgagcgg atcaaggcca ccgtgagcag
     3181 tgaaggcggc atcggcacga agttctcgaa ttggaacgca ccgtggtatc tcggcccggc
     3241 gatcgacgag ccggccttca agcacgagca tcacgagctg ctggcgctga ctgcgccgcg
     3301 gcctttcctg ctggtcggcg gagagtcggc cgatggcgac cgcggctggc cattcattgc
     3361 ggcggcgttg ccggtctatg aactgcacgg caagccggcc cgccttggcc agttcaacca
     3421 ccgcaaaggt catgcggtgc cgcccgaagc cgagcggcgg attgaggagt ggctgctcac
     3481 gtatttgtag gtcccgaccg cttgggcggg accggcggcc agtatcagtt cgtggcgctg
     3541 gtcccgccgt ggccgtcgga acctacactt catccgtccg ccacacgaaa aagaacatgt
     3601 actgcggcgt ccagagcagc agggccgcca gggcgacgag gatcacgacg gggccggtca
     3661 ggaccatctc ggccagcttc agcgtggcga tgaacagcac cagcagcatc aggttcgctg
     3721 ctgccgtccg aagctgcgtg gcccggatcg gcaggcattc ggagaaaagg taagcgaacc
     3781 acggcccgac ggcgaacacc agcaggcaat aatggaccgc cgccttgtag cccaccagcc
     3841 gatcgaaggc cagcgtgatc gcgaccatcg ccgtcacgat gagcaggctt tgcagtgaga
     3901 attgcaggag gggacgatgg gccgatgaac tcgtcattcc cgtattctac cgcacttata
     3961 cgcctagcgc accacgccca gctcttcctc caccgcgtcg atcgtctcgg catcgacctg
     4021 caccaggttg tggctggcgc cggccaggag ggcgagttcc gcgagctgat ttaccttccg
     4081 cggtgcgccc cccgacagct caaacagcct gcgaactgcg gcgtcgttga aggcgggctg
     4141 cttgcggccg gcctgggcga ggctggccag caggtactcg cgagtctcgt cctcattcca
     4201 gggagtcaga tcaattcgca gggagacttg atccactagg ccgcgggtaa gccgggcagc
     4261 cgattcgacc ctggccgtgg cgatgacggt gagcggagaa tcggcaatgc ccagcagggc
     4321 ttccactttc gcttgggcct cgccggtcgc ccggtcgagg ttgtcaaaca aaacggtcgc
     4381 acccaggcct tcgagcttca attcttccaa acgatccgtg actgccgccc acgggcagat
     4441 ggttgattcg ggagggcacc ccaggcccat ggccagtcct gccagcaggc ctttcgcgtc
     4501 ggagccggcg gcagcgacga gcgtcacggc cgatcctgtt cgctcggccc gccgcgccag
     4561 ttcgcccagc acgagcgact tcccgctgcc ggccgggccc aggaggagcc cgaagcgact
     4621 ccggctctcg atcagaaact ccagccgggc cagggcctcg gcgtgggtcg ggctgccttc
     4681 gagggagcgg cgggcggcat cgtccgagaa cggcccgcgc tgcaatcccc aataggcttc
     4741 gaacatcggc ggcctcagcg ggtaggacgg gtttgtaacc cgtctcgtct caattgggaa
     4801 ttcttaccag cgacgacagg ctcggacggc ttggaaagcc gtcctacgtc tgcgcggacg
     4861 ataccgtatc cgtcatgaga tcggtagaat cgcgggccga cttgaacgat tctgcaaact
     4921 tttccgcgcc aatcgcttac tggcacgtga acgacgatgt ccgagcgctt ctatgtcgag
     4981 ccgccgatcg acttaccgca tgtgacgctg accggcgatg aattccggca cctggccgcc
     5041 gtcatgcggg ccaggcccgg cgacgaagtg acgctcttcg acggcagcgg ggcggagttt
     5101 gccgcgcgga tcgtgcacat cggcaagcgc gaggcacggc tcgaaatcat cgagcgacgc
     5161 gaagtctccc gtgagttgcc gctggagctc acgctggctg tggcgctgcc caagggcgaa
     5221 cggcagaagt ggctggtcga aaagctcacc gagctgggtg tgacgcgtct tgtgccgctg
     5281 gtcaccgagc gtggggtcgc ccagccgacg attggcgccg tcgatcgcct gcgccgcagc
     5341 gtcatcgagg c
//
LOCUS       DASUGB010000024         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_280710, whole genome shotgun sequence.
ACCESSION   DASUGB010000024 DASUGB010000000
VERSION     DASUGB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280710"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFV87_00505"
     CDS             complement(<1..446)
                     /locus_tag="VFV87_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102259.1"
                     /translation="MNTLKSAALVVVLLGVLYGVYIVLSKPHMLALPGPTAQPGEDLG
                     PPLVDFSSAGEAGHDDHDHVQAPTTVNASADLPPRGGSYQPNLDSPAMLPPPSLPSTE
                     PPTDPTLASSAGEARHSSYESPEPPGSAAVPPLETSPPATNPPPDP"
     gene            754..1098
                     /locus_tag="VFV87_00510"
     CDS             754..1098
                     /locus_tag="VFV87_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX5102260.1"
                     /translation="MSGKTIFKRIIDKEIPAKIVYEDDHCLAFHDVSPQAPTHVLVIP
                     KREIPSLAEVSAGDQALLGHLLLTVKKLASDLGLSGGYRIVINCGPDGGQSVDHLHLH
                     LLAGRALSWPPG"
     gene            complement(1108..>2055)
                     /locus_tag="VFV87_00515"
     CDS             complement(1108..>2055)
                     /locus_tag="VFV87_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5102261.1"
                     /translation="TTEMEQRGLDQAIAMIQGSSGILTHWSVAGPLPANLERLDTYAS
                     DTASWQTLAATGIDAKVVLAPKDGPPGMMWLAVTTIAATDATDVQFLGSSSGGLHVFL
                     SDQQIYERQEPQAFRPDSDQFAARLAKGDNRVVVSVGPTKDPVEFHLRFRRKSATAEH
                     ERLIQAALSRSGNPERGRQVLLNVEKSLCLKCHKVGDQGERTGPELTGLGSRFSRIHI
                     VESILEPSRTIAPSYGTLSLILSDGRPLAGIKIAETATELTLVDNQAQKHTINKADID
                     QQQPSPLSTMPDGLEKRLSEEEFVDLAAYLASLKESPPP"
BASE COUNT          362 a          624 c          687 g          382 t
ORIGIN      
        1 tggggatcgg gtggggggtt ggtggccggc ggagaggtct ccagcggcgg aactgcggcg
       61 ctgccaggcg gctcgggcga ttcgtagctg ctgtggcggg cttcacccgc tgacgacgcc
      121 aaggttgggt cggtcggtgg ctcggtggag ggcaaactgg gtggaggcag catggccggc
      181 gaatcgaggt tcggctggta cgatccgccg cgcggcggaa gatccgccga tgcgttcacg
      241 gttgtgggag cctggacgtg atcgtggtcg tcatgaccgg cttcccctgc ggaggaaaaa
      301 tcaaccagcg gcggccccag gtcctcgccc ggctgggcag tgggaccagg cagcgcgagc
      361 atgtgcggct tgctgagcac gatgtaaacg ccgtacagca ctcccagcaa aacgaccacg
      421 agtgccgcgg acttgagcgt gttcacgttc gtcctccttg acgatcttca cgcgcgcgat
      481 agccgcccgg ctgcgccatg cgcacggcgt ctggggcaaa gaatccagcg ctgcgagatc
      541 ataggcagaa ggcgaagaca aggctagagc agtcgcactt gcggcagcgg gggcatgcta
      601 gcaggcccag aactgccctg tccgcttgag atgtacctcc cgccgcacga acagccacct
      661 gccggcccgt cgtgatgacc gccagctcgc cttagaatgc acccggggcg acgttcgcgg
      721 aatgaccact gactcaacat ggggcgatca acaatgagcg gtaaaacgat cttcaagcgg
      781 atcatcgaca aggaaatccc cgccaagatc gtgtatgaag acgatcactg tctagctttt
      841 cacgacgtaa gtccccaggc gccgacgcat gtgctggtca tccccaaaag ggaaattccc
      901 tcgctcgcgg aggtttcggc tggcgatcag gcgttgctgg ggcacctgct gttgaccgtg
      961 aagaagctgg caagcgatct gggtctgtct ggcggctacc gtatcgtcat caactgcggt
     1021 cccgacgggg ggcaaagcgt tgatcacctc cacctgcacc tgctcgccgg ccgggcgcta
     1081 tcgtggccgc cgggatagca cggcaaatca cggcggcggg gactctttga ggctggcgag
     1141 gtaggcggcc agatcgacga attcctcttc gctgagccgt ttttccaggc cgtcgggcat
     1201 cgtgctgagg ggcgagggct gctgctggtc gatgtccgcc ttgttgatcg tgtgcttctg
     1261 ggcctgatta tcgacgagcg tcagttccgt ggcggtctcg gcgatcttga ttcccgccag
     1321 cgggcggcca tcgctcagaa tgagcgacag cgtgccgtag ctcggcgcaa tcgtgcggct
     1381 gggctccagg atcgattcga cgatgtgaat ccgcgaaaag cggctcccca ggccggtcag
     1441 ctcggggccg gtgcgctccc cctggtcgcc gactttatgg cacttgaggc acagtgattt
     1501 ttccacgttc agcagcacct gccgcccgcg ctccggatta ccggatcgcg aaagggccgc
     1561 ctgaatgagc cgctcgtgct cggctgtcgc gctcttgcgg cggaaacgga ggtggaactc
     1621 gaccgggtcc ttcgtcgggc cgacggacac gacgacgcga ttgtccccct tggcgagcct
     1681 ggccgcgaat tggtcagaat cggggcgaaa tgcctgcggc tcttggcgtt cgtaaatctg
     1741 ctggtcactc aaaaagacgt gcaggccgcc gctgctggag ccgagaaatt gcacgtcggt
     1801 ggcatcggtc gcagcgatag tggtgactgc cagccacatc atgcctggtg gaccatcctt
     1861 cggcgcgaga acgactttcg catcgatacc ggtcgctgcg agcgtttgcc aagacgccgt
     1921 gtcgctcgca tacgtatcca agcgctccag gtttgccgga agcggcccgg caaccgacca
     1981 gtgcgtcagg atcccactgg aaccttggat catggcgatg gcctgatcca gcccccgctg
     2041 ctccatttcc gtcgt
//
LOCUS       DASUGB010000025         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1392529, whole genome shotgun sequence.
ACCESSION   DASUGB010000025 DASUGB010000000
VERSION     DASUGB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392529"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..396
                     /locus_tag="VFV87_00520"
     CDS             91..396
                     /locus_tag="VFV87_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102262.1"
                     /translation="MLKYVAIGAVFCGLIVLASDAQAGRRGCCCGYSGGTVVAPAPVP
                     PPPAPPTAAAPDSTRTFSYEPAMYQPTYGAYGSWVSGRAYESAINKSLGRGFSASRN"
     gene            complement(492..1760)
                     /locus_tag="VFV87_00525"
     CDS             complement(492..1760)
                     /locus_tag="VFV87_00525"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5102263.1"
                     /translation="MSQPQISPATTAPPAAEPARPASRQLGERVRNLRLSRLPERRTP
                     WVKIIGWIAAILIISLGGWWGYVQLVGSGGNGEAPGAASQKSGVGGRKTEDKGQELDN
                     PKSKIQNPKSAAAPAAGEVVLEVKGYIVPRQQILVSPKVNGMILKLDVEEGRQVQKGD
                     VLAVLESTEYQAEFDRAKANVRLMREKLRELENGARPEEILQAEAELRENEALLVQLE
                     ADWKRTDDLRRKNSVTQQEFELAESKYLAQKQRVERLRVALALWKLGPREERIEVAKA
                     DLAQAEAELVKAEWKLGNCTIRAPISGTILKKNAEEGNIVNPIAFNGSFSLCDMADLS
                     DLEVDLNVGERDISVVKVGQKCRIRTDAWPDRVYDGYVSRLMPIADRAKAAVPVRVKI
                     RVPAEEEGVYLKPEMGAIVTFLNEQDERRE"
     gene            complement(1897..>2679)
                     /gene="queA"
                     /locus_tag="VFV87_00530"
     CDS             complement(1897..>2679)
                     /gene="queA"
                     /locus_tag="VFV87_00530"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5102264.1"
                     /translation="HGVWQVLSKTRGKLEPGEAIVVVSWDVRQSVTLRLLTKLEGGVW
                     AVRPEPLGQPLEILGRVGRVPLPPYIRDSEMVEADVAQYQTVFASQPGAVAAPTAGLH
                     FTPELLDVLQRRGVQRELVTLHVGIGTFRPITAERLDEHVMHSEWCSLSPQTALQIDR
                     ARAAGKRLVAVGTTVARTLESAAASGQLQAFQGPTSLFIRPPYEFHAIDALLTNFHLP
                     KSTLLILVRTFGGDALIRRAYEQAITERYRFYSYGDAMLVLP"
BASE COUNT          426 a          841 c          860 g          552 t
ORIGIN      
        1 cagcgcttga aagtgtcagt tatcctggcg tgcggctgac tatcctgggt agggaatctg
       61 cggccggcgc ccgattttag gagcgctgcc atgttgaagt acgttgccat tggtgcggtc
      121 ttttgcggtt tgatcgtgct cgcctcggat gcccaggcgg gccgccgtgg ttgctgctgc
      181 ggctacagcg gcggcacggt cgtcgcaccc gcgccggttc cgccgccgcc ggctccaccg
      241 actgcggctg cccccgacag cacgcggacc ttttcctacg aaccggccat gtaccagccg
      301 acctacgggg cctatggaag ctgggtctcg ggtcgagcct acgagagtgc gattaacaaa
      361 tcgctaggcc gcgggttctc ggccagtcgg aactgagcct gttccattcg attcgttctc
      421 gctcccagga tccgatcctg ggagcgacct tttttcgagg ctacttcctt actccttgct
      481 tgaggcagcg actattctcg ccgctcgtcc tgctcgttaa gaaaggtcac gatcgctccc
      541 atctcgggct tgagataaac gccttcttcc tcggcgggga cgcggatttt cacgcgtacc
      601 ggaacggcag ccttcgcccg gtcggcgatc ggcatcagcc gtgagacgta gccgtcatag
      661 acacggtcgg gccaggcatc ggtgcggatg cggcacttct ggccaacctt aacgaccgat
      721 atatcccgct cgccgacgtt cagatcgacc tccaggtccg acagatcggc catatcgcag
      781 aggctgaagc tgccgttaaa ggcgatcggg ttgacgatgt tcccttcctc ggcgttcttc
      841 ttgaggatcg tgccgctgat cggggcgcgg atggtgcagt tgcccagctt ccactcggct
      901 ttgaccagtt cggcttcggc ctgggccaga tcggccttgg cgacttcgat ccgctcttcg
      961 cgcgggccga gtttccacag agccaaggcc acccgcagcc gctcgacgcg ctgtttctga
     1021 gccaggtatt tgctctcggc cagttcgaat tcctgctggg tgacggagtt tttccgccgc
     1081 aaatcgtcgg tgcgtttcca gtcggcttcg agctgcacga gcagggcctc gttttcgcgg
     1141 agctcggcct cggcttgcag tatctcctcg ggccgggccc cgttttccaa ctcgcggagc
     1201 ttttcgcgca tcagtcgtac gttggccttg gcccggtcga attcggcctg gtactcggtg
     1261 ctttccagga ccgcgagcac gtctcccttc tgcacctggc ggccttcctc gacgtcgagc
     1321 ttgaggatca tgccgttgac cttgggactg accagaattt gctgacgggg gacgatgtag
     1381 cctttgactt cgaggacgac ttcgccggcg gccggggcag cggcagattt tggattttgg
     1441 attttggatt ttggattgtc taattcctgt cccttgtcct ctgtcttccg acctccgacc
     1501 cctgacttct gactcgcggc ccctggcgcc tcgccattgc cgccagagcc cacgagctga
     1561 acgtaccccc accagccgcc gaggctgatg atcagaattg cggcgatcca gccgatgatc
     1621 tttacccaag gggtgcggcg ctcgggcagg cgcgagagtc ggaggttgcg aacccgctcg
     1681 ccaagttggc ggctagcggg gcgggccggt tcggcggcgg ggggcgcggt cgtcgcaggc
     1741 gagatttggg gctgactcac aggcgaatgg ctgggaaaga cggcagagaa ggcacatctc
     1801 tattatggca gagcagcggc tgttagccag cctgccggcg ggtgagtggt gagtagttga
     1861 gtgcccgtac tcaccactca cttactcacc tacccactac ggcagtacca gcatcgcgtc
     1921 gccgtaactg tagaagcggt aacgctcggt gatggcctgc tcgtaggcgc ggcggatgag
     1981 cgcatcgccg ccaaacgtgc ggacgaggat cagcagcgtg ctcttgggga ggtggaaatt
     2041 cgtcagcagg gcgtcaatgg cgtggaattc gtatggcggg cggatgaaga ggctggtagg
     2101 gccttgaaac gcttgcaact ggccggaagc tgccgcggat tcgagcgtgc gggcgacggt
     2161 cgtccccacg gcaacaaggc gtttcccggc cgctcgcgca cgatcaatct gcagggctgt
     2221 ttgaggcgac agcgagcacc attcgctgtg catcacgtgc tcatccagcc gctcggccgt
     2281 gatcggacgg aaggtgccga tgccgacgtg gagcgtgacc agctcgcgct gcacgccccg
     2341 ccgttgcagg acatcgagca attccggcgt gaagtgcagg ccggcggtcg gtgcggctac
     2401 cgctcccggc tgtgaggcaa agaccgtctg gtattgggcc acatcggctt caaccatttc
     2461 gctatcgcgg atgtacggcg gcagcggcac gcggcccaca cggccgagga tttccagcgg
     2521 ctggcccagc ggctccggac gcacggccca cacgccgccc tccagcttcg tcagcagccg
     2581 cagggtcacg gactggcgaa cgtcccacga cacgaccacg atcgcctcgc ctggctccag
     2641 cttgccgcgg gttttgctga gcacttgcca gacgccgtg
//
LOCUS       DASUGB010000026         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1253410, whole genome shotgun sequence.
ACCESSION   DASUGB010000026 DASUGB010000000
VERSION     DASUGB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253410"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VFV87_00535"
     CDS             <1..1310
                     /locus_tag="VFV87_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX5102265.1"
                     /translation="RDGAAALRYLKPFATCDEDFERIRPLRHPWIQRLLAAQHIVDCL
                     ANPQPCGPLARSLPEPLVQEIARLIKPDSTAANRLFRVVETEDQRYHAMAASILYASG
                     ESWRPTAPAVYRLRGGFFAEANWAGAVFGHTAGRHTDLTDADLTGANLSAVKADYVVL
                     NGARLTRAILRKASLAGASARNADFADADLTAADAPGMDLKLANLERAVLDSAIFRAA
                     NLQKTNLCGASFRDAVLSEASLRDSQIEGADFSGADLSWAHLTRLVLRTAILTGASFQ
                     GADLSHCDLEGVRLHAPSFRTATLRNAWFTGSMLPAADFREANLRDTGLADIHWEDAD
                     LRGADLRGCTFHMGSTRSGLVGSPYPGHGSKTGFYTDDYYDQGYKRPEEIRKANLRGA
                     DLRGAKLDGVDFYLVDLRDTRFDVSIAEHLQRCGAILADRATN"
     gene            complement(1426..1935)
                     /locus_tag="VFV87_00540"
     CDS             complement(1426..1935)
                     /locus_tag="VFV87_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102266.1"
                     /translation="MQFHLRTLFVVTFAVALLCGVVFAAPPIVCLPVLCAMLWIGPAI
                     WINGIIYGRGAWRPFFIGGVIVGLGPHLGALYYSYMLVVSLFSDVGELSDWFGSEARV
                     ANMITAAILLGPGALAFLGGLTGVATYWLLLPPKNERSPKPLAADEYLVVSGRLTTAP
                     VERSVQRVV"
     gene            complement(1975..>2924)
                     /locus_tag="VFV87_00545"
     CDS             complement(1975..>2924)
                     /locus_tag="VFV87_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5102267.1"
                     /translation="WDEAMERFGHDAPDLRFGCELIDCTDLAAAVDFRVFKNAAETGQ
                     KVRGITAPGGAAHYSRKLIDELTEHAKQFGAQGLAWFRVEPDGKLWSPISKNFTDEQL
                     VKFKERMGAKLGDICFFVADTWAVTCKALYALRKRIGVDLKLYDPKAMHFSWIVEFPM
                     FEFDKEENRWVSMHHPFTAPRPQDVPMLDSDPAQARAVAYDLVINGSEAGGGTIRIHD
                     QATQSKVFGLLGIDAETARNRFGFLLDALQMGAPPHGGIALGIDRLVMLFGCLDNIRE
                     VIAFPKTQKATDLMTGAPNEVEAKQLKELGIKVAAPEQK"
BASE COUNT          534 a          947 c          934 g          509 t
ORIGIN      
        1 tgcgcgacgg cgccgccgcg ctgcgatact tgaagccgtt tgccacgtgc gatgaagact
       61 tcgagcgcat ccgcccgctc aggcatccct ggatccagcg ccttctcgcc gcgcagcaca
      121 tcgtcgattg cctcgcgaac ccgcagccgt gcgggccact ggccagaagc ttgcccgagc
      181 cgctcgtcca ggaaatcgcg cggcttatca aacccgacag cacagcggcc aatcgcttgt
      241 ttcgcgtggt ggaaacggag gaccagcggt accacgccat ggccgccagc atcctgtacg
      301 ccagcggcga gagctggcga cccaccgcac ctgctgttta tcgcttgcgg ggcggattct
      361 tcgccgaagc caattgggcg ggcgctgtgt tcggccacac cgctggtcgg cacacggacc
      421 tgaccgatgc ggacctgacg ggagcgaatc tgtcggcagt gaaagcagat tacgtcgtac
      481 tgaacggcgc gcggcttact cgggcgatcc tgcgaaaggc atcgctcgcc ggagctagcg
      541 ctcgcaacgc ggactttgcc gatgccgacc tgaccgccgc cgatgcaccg ggaatggact
      601 tgaagctggc gaatctggaa cgtgccgtcc tcgatagcgc aatcttccgc gccgcaaatc
      661 tgcagaaaac gaatctgtgc ggcgccagtt tccgcgacgc ggtgctttcc gaggcttcgc
      721 tgcgagacag ccagattgag ggcgccgact tcagcggggc ggacctttct tgggcgcatc
      781 tgacacgcct agtcctgcgc acggcgatcc tgaccggggc ttcgttccag ggagccgact
      841 tgtcgcactg tgacctggaa ggggtccgtc ttcacgctcc cagtttccgc accgccacat
      901 tgcgaaacgc atggttcacg ggttccatgc ttcccgcggc ggacttccgc gaagccaatc
      961 tgcgcgatac gggcctggcc gatatccact gggaagacgc cgacctccgc ggggcggacc
     1021 tgcgcggctg cacgttccac atgggctcga cccgcagcgg actcgtcggc agcccgtatc
     1081 ccggccacgg cagcaagacg ggcttctaca ccgacgacta ctacgaccag ggctacaagc
     1141 ggcccgaaga aatccgcaaa gccaatctcc gcggcgccga cctccgcggc gcaaaacttg
     1201 acggcgtcga tttctacctc gtcgatctcc gcgacacccg gttcgacgtc agcatcgccg
     1261 aacacctgca gaggtgcggg gcaatcttgg cggatcgcgc gacgaactag gctaagtttg
     1321 gtttgaacac cggccagggt ggccgggttt ttgcgaagca ataacccgga ttgctggggt
     1381 agagctatcg ctcaacccca ggcaccccaa cgccttcacg gtttatcaaa ccaccctctg
     1441 aactgaccgt tccaccgggg cggttgtcag gcggccggag acgacgaggt attcgtcggc
     1501 ggcgagcggc ttgggcgagc gttcgttctt gggcggcagg agtagccagt aggtggccac
     1561 gccggtgagg ccgccgagga aggccagcgc gccagggccg agcaggatcg ccgcggtgat
     1621 catgttcgcg acccttgcct cggaaccgaa ccaatcgctc agttcgccga cgtccgaaaa
     1681 cagactgacg accagcatgt agctgtaata aagcgcgccc aggtgcggtc cgaggccgac
     1741 aatgacgccg ccgatgaaga acggccgcca cgcgccgcgg ccatagatga tgccgttgat
     1801 ccagatcgcc ggtccgatcc agagcatcgc gcacagcacc ggaaggcaca cgatcggcgg
     1861 cgcggcaaac accacgccgc agagcagcgc caccgcgaac gtcaccacaa acagcgtgcg
     1921 aaggtgaaac tgcatccgtg cctccaagaa tgaatatgag ttgcgcgggc gtgcctattt
     1981 ctgctccggt gccgccacct taatccccaa ctccttgagt tgtttcgcct cgacctcatt
     2041 cggcgcgccg gtcatcaggt cggtggcttt ttgggttttg gggaaggcga tgacttcgcg
     2101 gatgttgtcc aggcagccga agagcatgac gaggcggtcg atccccaggg cgatgccgcc
     2161 gtggggcggg gcgcccatct gcagggcatc gagcaggaag ccgaagcggt ttcgggccgt
     2221 ttcggcgtcg atgcctaaga ggccgaagac cttgctctgc gtcgcttggt catgaattcg
     2281 gatcgtgccg ccaccggctt cgctgccgtt gatgaccagg tcgtaagcga cggcgcgagc
     2341 ctgggccggg tcgctgtcga gcatcggcac gtcctgcggc cgcggggcgg tgaacgggtg
     2401 gtgcatgctg acccaacggt tctcttcctt gtcgaactcg aacatcggga actcgacaat
     2461 ccacgagaag tgcatcgctt tcggatcgta cagcttcaga tcgacgccga tccgcttgcg
     2521 cagggcatac agggccttac aggtcacggc ccaggtgtcg gcgacgaaga agcaaatgtc
     2581 gccgagcttc gcgcccatcc gctccttgaa cttcacgagc tgctcgtcgg tgaagttctt
     2641 gctgatcggc gaccagagct tgccgtccgg ctcgacgcgg aaccaggcca gaccctgagc
     2701 gccgaactgc ttggcgtgct cggtcagctc gtcgatcagc ttgcggctat agtgcgccgc
     2761 gccgccgggg gcggtgatgc cgcggacttt ctggccggtt tcggcggcgt ttttgaagac
     2821 gcggaaatcg accgcggcgg ccaggtcggt gcagtcaatc agctcgcagc cgaaccgcag
     2881 gtcgggagcg tcgtggccga accgttccat cgcctcgtcc cagg
//
LOCUS       DASUGB010000027         5093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1253456, whole genome shotgun sequence.
ACCESSION   DASUGB010000027 DASUGB010000000
VERSION     DASUGB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253456"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2812
                     /gene="uvrA"
                     /locus_tag="VFV87_00550"
     CDS             <1..2812
                     /gene="uvrA"
                     /locus_tag="VFV87_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5102268.1"
                     /translation="GSGKTSLALDTLYAEGQRRYIESFSLYTRQFLQRLDKPAAERID
                     GIPAAIAVTRNDVSRSNRATIGTATEIVDYLRLLYAKVGQLVCPQCGVAVERFSPQSA
                     AERLGELPEGTRLMIGFMAEAEPGESAEQLAARLQAAGFVRMHPHSGPLPEGEGATAA
                     ERHGGRSLQVIVDRLTAGAVSAQRLRDSLETAFNHGGGRAVAFVEAEALAGETPTPRV
                     TIDDRNWQRITFSTRLECPSCHREFADPEPRRLSFNSPLGACPTCEGFGNVSDLDMAL
                     IVPDPSKSIREGAIAPWNTPAYKHELEELLALAPDYGIPVHQPFSELSEEHLRLIREG
                     VPERNFGGLRGFFRWLEKRKYKLHLRVYLNRWRSSKVCPTCSGKRLNEEALAWRIGGL
                     NIAELTQLAINEALEFLTQSRDQWSRNAAGRNNRSQDAGNRDQWSRLWTALPQIRSRL
                     GFLQQVGLGYLSLDRTLRTLSGGEGQRVSLTSALGSGLVNMLYVLDEPSVGLHPSDVQ
                     KLVGAIQGLRDRGNSVVVVEHEEGLLRAADQLIEIGPGAGDRGGTVVFQGSLDELLQP
                     GASLTGEFLAGRRGISIPEHRRPTSRGRIKLLAARGHNLQNLTVDFPLGCLCVVTGVS
                     GSGKSSLVQETLYGAICRRKKKDCEEPLPYDEIVGDGQIDDVVLVDQSPIGRSPRSNP
                     VTYIKAFDPIRAVFADTVDAKMHNYTAGHFSFNVEGGRCDACQGEGYTQVDMQFLADI
                     YMKCGQCGGTRYRQEILAVKYRGQSIADVLAMTVREAFSFFRSQQKVQARLKQLIDVG
                     LDYLQLGQPANTLSAGEAQRLKLAAYLSAASKSRTLFLLDEPTTGLHPADVVTLLDCF
                     DALLAVGHSLVVVEHNLQLMRAADHLIDLGPGAGDDGGRLVAQGTPEEVARCPDSITG
                     RHLAESFVTCHWSFANSTTTDK"
     gene            2830..3756
                     /gene="folP"
                     /locus_tag="VFV87_00555"
     CDS             2830..3756
                     /gene="folP"
                     /locus_tag="VFV87_00555"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5102269.1"
                     /translation="MTTDNRPTSWKLRTRTLQFGRLPLLMGIVNVTPDSFSDGGQFLD
                     RRAAVAQALRLVEEGAEILDIGGESTRPYSTPVNEAEELARVVPVISQIRQHLPEAVL
                     SIDTSKATVAKAAIEMGVQIINDVTGLTGDPAMLEVARSSGAGVCAMHMQGTPQTMQD
                     NPFYDDVVEDIYMYLCQRRDELLAAGIDRERLCLDPGIGFGKTHQHNLTLMAHCDRFH
                     ELGCPLLVGHSRKGFIGKLIDLSKPRPVVAVPAESPEMTSDRTYGTIGGALALARQGV
                     QILRVHDVRAVREALLVFDACGGIDGQELRLS"
     gene            4147..>5093
                     /locus_tag="VFV87_00560"
     CDS             4147..>5093
                     /locus_tag="VFV87_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX5102270.1"
                     /translation="MSGSTARLEAIKAVTNYKPNGAPLNFKELSSKDVYGANVFSMAE
                     MQKRLPKPVFKSLKKTIEAGAKLDPSIADVVATAMKEWAIEKGATHYAHVFYPLTGLT
                     AEKHDSFLSPTSEGRAVAEFSGAQLAQGEPDASSFPSGGIRATFEARGYTAWDVTSPA
                     YILENPNGTTLCIPTAFVSWTGEALDKKTPLLRSMKALNVQAQRILKLLGHKDVAHVA
                     ATAGPEQEYFLIDRNFFFARPDLLNAGRSLFGAKPPKGQEFEDQYFGAIRERVLACML
                     ETEHELFKLGIPVKTRHNEVAPSQFEIAPVFEAGNVATD"
BASE COUNT          924 a         1703 c         1617 g          849 t
ORIGIN      
        1 cggcagcggc aagacaagcc tggccctgga cacgctttat gccgaaggcc agcgacgcta
       61 catcgagagt ttctcgctct atacgcggca attcctgcag cggctcgaca agccggccgc
      121 ggagcggatc gatggcattc cggcggcgat tgccgtgacg cggaacgacg tctcgcggtc
      181 caaccgggcc acgatcggca cggcaaccga aatcgtcgat tatctgcggc tgctgtatgc
      241 caaggtcggc cagttggttt gcccgcagtg cggcgtggcg gtggagcgat tctcgccgca
      301 atcggccgcc gagcgattgg gcgaattgcc cgaagggacg cggctgatga ttggcttcat
      361 ggcggaggcc gagccgggcg aatcggccga gcagctcgcg gcacgattgc aggcggcggg
      421 gttcgtgcgg atgcaccctc actccggccc tctcccagag ggagagggag cgaccgccgc
      481 ggaacgccac ggagggcgtt ccctacaggt tattgtcgat cggctgacgg cgggagcggt
      541 gtcggctcag cggctgcgcg attcgctgga gacggcgttc aatcacggcg gcggacgagc
      601 ggtggcattc gtggaggcgg aagcgctggc aggcgagacg cctaccccac gagtcaccat
      661 cgacgatcgc aattggcagc gcatcacgtt ttcgacccgc ctggaatgcc cctcctgcca
      721 ccgcgagttc gccgatcccg agccgcggcg cttgagcttc aacagcccgc tcggcgcttg
      781 ccccacgtgc gaaggcttcg gcaacgtcag cgatctcgat atggcgctga tcgtccccga
      841 tccttcgaaa tcgatccgcg aaggggcgat cgctccctgg aacacgcccg cctacaagca
      901 tgagctggaa gagctgctcg ctctggcccc cgactacggc attcccgtcc atcagccgtt
      961 cagcgagctg agcgaggagc acctgcggct gattcgcgag ggggtccccg agcgaaactt
     1021 cggcggcctg cgcggcttct tccgctggct ggagaagcga aaatacaagc tgcacctgcg
     1081 ggtctatctc aaccgctggc ggagctcgaa ggtctgtccc acttgcagcg gaaagcggct
     1141 gaatgaggag gcgctggcgt ggcgaattgg cgggctgaac atcgccgagc tgacgcagct
     1201 tgcgatcaac gaggcgctgg agttccttac tcaaagccgc gaccagtggt cgcggaatgc
     1261 ggccggccgc aacaaccggt cgcaggatgc ggggaaccgc gaccaatggt cgcggctttg
     1321 gaccgcgctc ccgcaaatcc gttcccggct gggctttttg cagcaggtcg gcctgggcta
     1381 cctctcgctc gaccgaacgc tgcgcacgct cagcggcggc gaagggcagc gggtcagcct
     1441 cacctcggcc ctcggcagcg gcctcgtcaa tatgctctac gtgctcgacg agccgtcggt
     1501 cggcctccat ccgagcgacg tgcaaaagct ggtcggcgcg attcagggtc tgcgcgatcg
     1561 cggcaacagc gtcgttgtcg tcgaacacga agaaggcctg ctgcgagccg cggatcaact
     1621 gatcgaaatc ggcccgggcg ctggcgatcg cggcggcacg gtcgtctttc agggctcgct
     1681 cgatgaactt ctccagcccg gcgcgagcct caccggcgag ttcctcgccg gccgccgcgg
     1741 catctccatt cccgaacacc gccgccccac cagccgcggc cggatcaagc tcctcgccgc
     1801 tcgcggccac aacctgcaga acctgaccgt cgatttcccg ctcggctgcc tgtgcgtggt
     1861 caccggcgtc agcggcagcg gcaaaagctc gctcgtccag gagacgctgt atggcgcgat
     1921 ctgccgccgc aagaagaagg attgcgaaga gccgctgccc tatgacgaga tcgtcggtga
     1981 cgggcagatc gacgacgtgg tcctcgtcga tcagtcgccc atcggccgct cgccgcgttc
     2041 caacccggtg acgtacatca aagccttcga cccgatccgg gcggtcttcg ccgacaccgt
     2101 cgatgccaag atgcacaact acacagccgg ccacttcagc ttcaacgtcg aagggggccg
     2161 ctgcgacgcc tgccagggcg agggctacac gcaggtcgat atgcagttcc tggccgacat
     2221 ctacatgaag tgcggccagt gcggcggcac gcgctaccgc caggagattc tcgccgtcaa
     2281 ataccgcggc cagagcatcg ccgacgtgct ggcgatgacc gtccgcgaag ccttctcctt
     2341 cttccgcagc cagcaaaaag tgcaggcccg cctcaagcag ctcatcgacg tcggcctcga
     2401 ctacctgcag ctcggccagc ccgccaacac gctctcggcc ggcgaggccc agcggctgaa
     2461 gctggccgcc tacctctccg cagccagcaa gagccgcacg ctgttcctcc tcgacgagcc
     2521 gaccaccggc ctgcacccgg ccgacgtcgt gacgctcctc gattgcttcg acgcgctcct
     2581 cgccgtcggc cactcgctgg tcgtcgtcga gcataacctc cagctcatgc gggccgccga
     2641 ccacctcatt gatctcggcc ccggcgcggg agacgatgga ggcagactcg tggctcaggg
     2701 gacgccggaa gaggtggccc gctgcccaga ttcgattacc ggccgccatc tcgcggagtc
     2761 atttgtcact tgtcattggt catttgcgaa ttcaacgacc acagacaaat gacaaatgac
     2821 aaatgacaaa tgaccacgga caaccgcccc acctcctgga agctccgcac ccgcacgctg
     2881 cagttcggcc ggctgccgct gctgatgggc atcgtcaacg tcacgcccga cagcttcagc
     2941 gacggcgggc agtttctcga tcggcgggcg gcggtggcgc aggcgctgcg gctggtggag
     3001 gaaggggcgg agatcctcga catcggtggc gagagcacgc ggccgtattc cacgccggtc
     3061 aacgaagcgg aagaattggc ccgcgtcgtc ccggtgatct cgcagatccg ccagcacctg
     3121 ccggaggcgg tgctctcgat cgacacgtcc aaggccaccg tggcgaaagc ggcaatcgag
     3181 atgggcgtgc agatcatcaa cgatgtgacg gggctgacgg gcgacccggc gatgctcgaa
     3241 gtggccaggt ccagcggcgc gggcgtctgc gcgatgcaca tgcaaggcac gccgcagacg
     3301 atgcaagaca acccgttcta tgacgacgtg gtcgaagaca tctatatgta cctctgccag
     3361 cggcgcgacg agctgctcgc agccggcatc gaccgcgagc ggctctgcct cgaccccggc
     3421 atcggcttcg gcaagacgca ccagcacaat ctgacgttga tggcccattg tgaccggttc
     3481 cacgagctgg gctgcccgct gctggtcggc cattcgcgga agggcttcat cgggaagctg
     3541 atcgacctct caaagccgcg accagtggtc gcggttcccg ctgaatcgcc tgagatgacc
     3601 tccgaccgta catacggcac catcggcggc gcactggccc tcgcccgcca gggcgtgcag
     3661 attctccgcg tccacgacgt gcgcgccgtc cgcgaagccc tgctcgtctt cgacgcctgc
     3721 ggcgggatcg acggccagga gctgcggcta tcatgatccc gcaactcagg acggcgaacc
     3781 tgcaattttg ctaatagggt gcttgtcaga gtcgcggtcg cgccgtacaa ccatccacgc
     3841 gaagatcctt cgattgcgcg ccggatgcgc cgagcgattt gaacgacccg tctcgctcgc
     3901 tgaaaggatt tctgcctgca cattgggcat gcgtgccctg caagcgggca atccaagtat
     3961 ctgcggcggc ttcgacttgg cagcggctgc agcgcaagtc catgctcatc aaggactttc
     4021 gccagccttc gtaagccggg gccggcaagt ggtgttttgg cacatcggat gcttcttggc
     4081 gaaagtttct ggacatccgt ccgccgcgtt tcctgtttcg agtctttagg gtaggagtgg
     4141 tcttccgtga gtggtagcac agcgcgtctc gaggccatca aggccgtcac caactacaag
     4201 cccaacggcg ccccgctgaa tttcaaggag ctgtcgtcca aggatgtcta tggggccaat
     4261 gtcttctcca tggccgaaat gcagaagcgg ctgcccaagc cggtcttcaa gtcgctcaag
     4321 aagaccatcg aagcgggggc gaagctcgat ccgagcatcg ccgacgtggt cgccaccgcg
     4381 atgaaagagt gggcgatcga aaagggggcc acccactatg cccacgtctt ttatccgctg
     4441 accggcctga ccgccgagaa gcacgacagc ttcctctcgc ccacgagcga agggcgggcg
     4501 gtggccgagt tttccggcgc gcagctcgcc cagggtgagc ccgacgcctc cagcttcccg
     4561 tccggcggca tccgggcgac gttcgaggcc cgcggctaca cggcctggga cgtgaccagc
     4621 ccggcttaca tcctggaaaa ccccaacggc accacgctct gcattcccac ggccttcgtg
     4681 agttggaccg gcgaggcgct cgacaagaag acgccgctcc tgcggtcgat gaaggccctg
     4741 aacgtgcagg cccagcggat tctcaagctc ctggggcaca aggacgtcgc ccacgtcgcc
     4801 gcgaccgccg gccccgagca ggaatacttc ctgatcgacc gcaatttctt cttcgcccgg
     4861 cccgaccttt tgaatgccgg ccggtcgctc ttcggcgcca agccgccgaa gggacaggag
     4921 ttcgaggacc agtacttcgg tgcaattcgc gagcgcgtgc tggcctgcat gctggagacc
     4981 gagcacgagc tgttcaagct cggcattccg gtcaagacgc ggcacaacga agtggcaccg
     5041 agccagttcg aaattgcgcc ggtgttcgag gccggtaacg tcgcgaccga tca
//
LOCUS       DASUGB010000028         5408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_142434, whole genome shotgun sequence.
ACCESSION   DASUGB010000028 DASUGB010000000
VERSION     DASUGB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142434"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VFV87_00565"
     CDS             <1..1567
                     /locus_tag="VFV87_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102271.1"
                     /translation="VNPPEKPLDAIYGDKTDKKRVTGSIEFALDRKDETAWTNDIGPG
                     RTNVPRKAVFTLEKPLSFPSGTQLKFRLTQNHGGWNSDDNQNNNLGRFRFAVTSAADA
                     KADPAPAAVRKVFETPIDERSPAQVAAVFRYWRTTVPEWKEANEQIEALWQQHPRGTS
                     QYTLRAREDHRQSFVLARGDFLKPTTPVSAGTPGFLHPLGAENPTRLDFARWLVDEKG
                     PTAARAIVNRVWQTYFGTGLVATSEDFGLQGEAPSHPELLDWLTIDFIEHGWSLKHLH
                     KLIVSSATYQQLSKVTAELLARDPNNRLLARGPRFRMEAEAVRDIALAASGLLNSKLG
                     GPPVYPPAPEFLFVPPASYGPKVWKEEKGPDRYRRALYTFRFRSVPYPVLQTFDAPNG
                     EFACVRRVRSNTPLQALASLNEPLFLECARALAMKTLAEGGATDVERLAFAFRRCTAR
                     VPDETESAALLALLNEQQERFSRTDAKPWDLAANDPANPPALPDGASPAQAAAWTAVC
                     RVVLNLDETITKE"
     gene            1595..3031
                     /locus_tag="VFV87_00570"
     CDS             1595..3031
                     /locus_tag="VFV87_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102272.1"
                     /translation="MNCQSHLDHGCHPTSLARRWFLQQCGVGLGAAALAELAGSGVSA
                     APLPANPLAPKPPHFAGKAKSVIYLFMAGAPSHLELFDNKPELAKWDGKLPPAELLKG
                     YRAAFINPNSKLLGPKFKFAKYGNCGAELSELLPHLATVVDDIAIVKSMVTDAFNHAP
                     AQILMNTGNQQFGRPSMGAWVTYGLGSETQDLPAFVVFSSGKKGPSGGNSNWGNGFLP
                     TVYQGVQFRGGSEPVLFLANPRGVDRQLQADSLDAIRALNQHRLEQVGDPEIATRTAA
                     FEMAFRMQASAPDLMDLSSEPKETLEMYGAEPGKSSFANNCLLARRLVERGVRYVQLF
                     HEAWDQHGNLVADLKKNCEDTDKPSTALIKDLKQRGLLDQTLVIWGGEFGRTPMVQGG
                     NDGRDHHPNAFTYWLAGGGIKPGITLGESDEFGFNATRDRVHVRDLHATVLHCLGLDH
                     QRLTYKFQGLDAKLTGVEPARVVKEILA"
     gene            complement(3338..>5408)
                     /gene="priA"
                     /locus_tag="VFV87_00575"
     CDS             complement(3338..>5408)
                     /gene="priA"
                     /locus_tag="VFV87_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5102273.1"
                     /translation="WMADYYLCPLGQVLQAIVPAGVRGQAGTREMTFLSVPEAVKQQL
                     AAGTLKLGAKQLDALRILAAAPRPLSPPELAQVAKCTLGPIGELRKKKLVKAEVRRIQ
                     QVDVEDAPLPREEHLVLNPDQQAALDCILAALRERRHETILMHGVTGSGKTEVYIRAI
                     EEVIHYGRQAIVLVPEISLTPQTRSRFKSRFSRVAVLHSHLSDAERHWHWQQIARGDV
                     EVVVGARSAVFAPTPNLGLIVIDEEHDGSFKQGESPRYHARDVALQRAAMESVPLVLG
                     SATPSLESWQRATQGTGDRGQGTGEAIRNPEFKIQNPAASEPRTLNPEPFRRIDLPRR
                     VEDRPLPAVATIDLRTEFQSRASRGAVSRSLHRAIDQAIGEGGQVILLLNRRGFSTHI
                     QCPACGHVVRCPHCDLALTHHRADEQALCHYCDFQMPAPVRCPQCAFDGIRFTGFGTE
                     RLEAEIKSRFPGVETLRMDSDTMQKPGSHEKALARFRAGEVKILLGTQMIAKGLDFPN
                     VTLVGVVNADTTLHFCDFRAAERTFQLVTQVAGRTGRGDKGGRVLVQTFSPDHYAILA
                     AIDHDYARFAAAELPSRQEHLYPPFASLIRLIFRGESEPLTEQFAEAAAGKLRTAIEA
                     ANIEHRLLGPAPCPIAKLRGLFRYHLLASSPAADQLRTAVRTVIDTLEPVEGVQFVVD
                     VDPLDLL"
BASE COUNT          976 a         1778 c         1722 g          932 t
ORIGIN      
        1 cgtgaatccg ccggagaagc cgctcgatgc gatctatggc gacaagacgg acaagaagcg
       61 ggtgacgggg tcgatcgaat tcgccttgga tcgcaaggac gaaacagcct ggacgaacga
      121 tatcggcccc ggccggacga acgtgccgcg caaggccgtc ttcacgctcg aaaagccgct
      181 cagcttcccc agtggcacgc aactcaagtt ccgcctgacg cagaaccacg gcggctggaa
      241 cagcgacgac aaccaaaaca acaatttggg ccgctttcgc tttgccgtca catccgccgc
      301 cgacgcgaaa gccgatcctg cccccgcggc tgtccgcaag gtcttcgaga cgcccatcga
      361 cgagcgctcg ccggcccagg tcgccgcggt tttccgctac tggcggacga ccgtgccgga
      421 gtggaaggaa gccaacgagc agatcgaggc cctttggcaa cagcacccgc ggggcacgtc
      481 acaatacacg ctaagggcgc gcgaggacca tcgccagtca ttcgttctgg cgcgtggaga
      541 ttttctgaaa ccgacgacgc cggtgtcggc gggtacaccg ggtttcttgc atccgctcgg
      601 cgcggagaac ccgacccgct tggactttgc caggtggctg gtcgatgaaa aaggtccgac
      661 tgcagcgagg gcgatcgtca atcgcgtctg gcaaacttac ttcggcacgg ggctcgtcgc
      721 gaccagcgaa gatttcggat tgcagggcga agcaccttcg catccggaat tgcttgactg
      781 gctgacgatc gattttatcg agcacggctg gtcactgaag catttgcaca aactgatcgt
      841 ctcctcggcc acgtatcagc agttgtccaa ggtcactgct gagctcctgg cccgcgatcc
      901 gaacaaccgc ctgctcgccc gcggcccgcg gttccggatg gaagccgaag ccgtgcgcga
      961 catcgccctg gccgcgagcg ggctgctcaa ctcgaagttg ggcgggccgc ccgtgtatcc
     1021 ccccgcgccg gagttcctgt tcgttccgcc ggcgagttac gggccaaaag tctggaagga
     1081 agagaagggc ccagaccgtt atcgtcgggc gctctacacg ttccgcttcc gcagtgtccc
     1141 ttatcccgtg ttgcaaacat ttgacgctcc caacggcgag ttcgcctgcg tccgccgcgt
     1201 ccgcagcaac acgccactcc aggccctcgc ctcgctcaat gagccgctgt tccttgaatg
     1261 tgcccgcgcc ttggcgatga agacactcgc cgaaggcggc gcgaccgacg tcgagcgcct
     1321 cgccttcgcc ttccgccgct gcacggcccg agtgcccgat gaaacagaat ccgcagcgct
     1381 gctcgcgctc ctgaatgagc agcaagagcg cttcagccgg actgacgcca aaccctggga
     1441 tctcgccgcg aacgaccccg ccaatccgcc ggccttgccc gacggcgcct cgcccgcgca
     1501 agccgccgcc tggacagccg tttgtcgcgt agttttgaat ctcgacgaaa ccatcaccaa
     1561 agaatagctg gattcctacg cccgaaatga aaccatgaac tgccaatcgc atctcgacca
     1621 cggctgccat ccaacctcgc tcgcccgccg ctggtttctg cagcagtgcg gtgtcggcct
     1681 tggcgcggcg gcgctggccg agttagcagg cagtggcgtt agtgccgcac cgctgccggc
     1741 caatccgctc gcgcccaagc cgccgcactt tgccggcaaa gcgaaaagcg tgatttacct
     1801 cttcatggcc ggggcgccga gccatttgga gctgttcgac aacaagccgg aactggccaa
     1861 atgggacggc aagctgccgc cggcggagct cttgaaaggc taccgggcgg cattcatcaa
     1921 tcccaatagc aagctgctgg ggccgaagtt caagttcgcc aaatacggca actgtggggc
     1981 ggagctctcg gagctgctgc cgcacctggc gaccgtcgtc gatgacatcg cgattgtcaa
     2041 aagcatggtg accgacgcct tcaaccacgc gccggctcag atcctgatga ataccggcaa
     2101 ccagcagttc ggccggccaa gcatgggggc ctgggtcacc tatggcttgg gaagcgaaac
     2161 acaagacctg cccgcgttcg tcgtcttctc cagcggcaaa aaaggcccca gcggcggcaa
     2221 cagcaactgg gggaacggct ttctgccgac cgtctatcag ggcgtgcaat tccgcggcgg
     2281 cagcgagccg gtgctctttc tggccaatcc ccgcggcgtc gatcggcaat tgcaggcaga
     2341 ttcgctggac gcgatccgcg cgctcaacca gcatcggctc gagcaggtcg gcgacccgga
     2401 gatcgccacc cgcaccgcgg cgttcgagat ggcctttcgc atgcaggcca gtgctcccga
     2461 cctcatggat ctctccagcg agcccaagga gacgctcgag atgtacggcg cggagcctgg
     2521 caagagctcg ttcgccaaca actgcctgct cgcccggcgc ctcgtggagc ggggggtccg
     2581 ctacgtgcag cttttccacg aagcgtggga ccagcacggc aacctggtcg ccgacctcaa
     2641 gaagaactgt gaagacaccg acaagccatc gaccgcactg atcaaagacc tcaagcagcg
     2701 cgggctcctc gatcagacgc tcgtcatctg gggcggcgaa ttcggccgca cgccgatggt
     2761 ccaggggggc aacgacggcc gcgatcacca tcccaacgcc ttcacctact ggctcgccgg
     2821 cggcggcatt aagcccggca tcacgctcgg cgagagcgac gaattcggct tcaacgccac
     2881 gcgcgaccgc gtccacgtcc gcgacctcca cgccaccgtc ctccactgcc tcggcctcga
     2941 ccaccagcgc ctcacctaca aattccaagg cctcgatgcc aagctcaccg gcgtcgagcc
     3001 ggcgcgggtg gtgaaggaga ttctggcgta gccgtggggt aggcgtctcg cctgtccgac
     3061 tcatccctct ggactggcgg taccggcctc tgggtgcaag ttccattcat ccgctgtgta
     3121 ccagcgcaag tctgctcgca gccggaatgc atgaggtgat ttgcaatcaa gtccgccagc
     3181 ggaccgccgg gcggatcgtt gttctcgcag tcctttggga atccgccgcg gaagctcgcg
     3241 tgcgtgcgca tcattacttc ttcctttgtt gccttcgtta ccttctgttt gcgattttct
     3301 aggaaaaatg aacagaagga aacaaagata acgaaggcta aagcaaatcc agtggatcga
     3361 catcgacgac gaattggacg ccttcgaccg gttcgagcgt gtcgatgacg gtgcggacgg
     3421 cggtgcggag ttggtcggcg gcggggctgc tggcgaggag gtggtagcga aacagcccgc
     3481 ggagcttggc aatcgggcaa ggggcggggc cgaggaggcg gtgttcgata ttggcggctt
     3541 cgatggccgt gcggagtttg ccggcggcgg cttcggcgaa ctgctcggtg aggggctcgc
     3601 tttcgccgcg gaagatcagt cggatcagag aagcgaaggg cgggtagagg tgctcctgcc
     3661 gcgacggaag ctcggcagcg gcgaagcgcg catagtcgtg gtcgatggcg gcgaggatcg
     3721 cgtagtggtc gggcgaaaac gtctgtacca ggacgcggcc ccccttgtcg ccgcggccgg
     3781 tgcggccggc gacctgcgtc acgagttgaa acgtccgctc ggctgcccgg aagtcgcaga
     3841 agtggagcgt cgtgtcggcg ttcacgacgc cgaccagcgt cacgttcgga aaatcgagcc
     3901 ccttagcgat catctgcgtg ccaagcagga tcttcacctc gccggcacga aaccgcgcaa
     3961 gggccttttc gtggctgccg ggcttctgca tcgtgtcgct gtccatgcgg agcgtttcga
     4021 cgccgggaaa gcgcgacttg atctccgcct ccaagcgctc ggtgccaaag cccgtgaagc
     4081 ggatgccgtc gaacgcgcac tgcgggcagc ggaccggggc gggcatctgg aaatcgcagt
     4141 agtggcagag cgcctgctcg tcggcccgat gatgcgtgag ggccagatcg cagtgcgggc
     4201 agcggacgac gtgcccgcac gccgggcact ggatgtgcgt gctgaatccg cggcgattga
     4261 gcagcaagat cacctgcccg ccctcgccga tcgcctgatc gatcgcccgg tgtagcgacc
     4321 ggctcaccgc cccgcggctg gcgcggctct ggaactccgt ccgcaagtcg atcgtcgcca
     4381 ccgccggcaa cgggcggtct tcgacgcggc ggggaaggtc gatgcggcgg aagggttcag
     4441 ggttcagggt tcggggttca gaggcggcgg gattttggat tttgaattct ggatttcgga
     4501 ttgcctcccc tgtcccctgt cccctgtccc ctgtcccctg cgtagctcgc tgccacgatt
     4561 cgagcgacgg cgtcgcactg cccagcacga gcggcacgct ttccatggcg gcccgctgga
     4621 gggcgacgtc gcgggcatgg tatctcggcg attcgccctg cttgaatgag ccgtcatgct
     4681 cttcgtcgat cacgatcagg ccgagattcg gcgtcggggc aaagacggcg ctgcgggcgc
     4741 cgacgacgac ttccacgtcg ccccgcgcga tctgctgcca atgccaatgc cgctcggcat
     4801 cggagaggtg cgaatgcagc accgccacgc ggctgaagcg ggacttgaac cgcgagcgag
     4861 tctgcggcgt caggctgatc tccggcacca ggacgatcgc ctgccggccg taatgaatta
     4921 cctcctcgat cgcccggata tagacctccg tcttgccgct gccggtgacc ccgtgcatga
     4981 ggatcgtctc gtgccggcgt tcgcggagcg cggccagaat gcaatccagc gccgcctgct
     5041 gatcgggatt gagcacgaga tgctcttccc gcggcagcgg cgcgtcctcg acatcgacct
     5101 gctgaatccg gcggacctcg gccttcacga gcttcttctt ccgcaattcg ccgatcggcc
     5161 cgagcgtgca tttcgctacc tgggccagtt cgggcgggct cagcggacgc ggcgcggcgg
     5221 cgagaattcg caaggcatca agttgctttg cgcccagctt gagcgtcccc gccgcgagtt
     5281 gctgcttgac cgcttcgggc acgctgagga acgtcatctc ccgcgtgccg gcctgcccgc
     5341 ggacgccggc gggcacaatc gcttgcagca cctgccccag cggacagagg taatagtcgg
     5401 ccatccac
//
LOCUS       DASUGB010000029         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1114914, whole genome shotgun sequence.
ACCESSION   DASUGB010000029 DASUGB010000000
VERSION     DASUGB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114914"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..377
                     /locus_tag="VFV87_00580"
     tRNA            304..377
                     /locus_tag="VFV87_00580"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:338..340,aa:Met,seq:cat)
     gene            787..>2254
                     /locus_tag="VFV87_00585"
     CDS             787..>2254
                     /locus_tag="VFV87_00585"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5102274.1"
                     /translation="MAREINQPSLAPAVSRMPRIVFSSAALLVALAASPLLAEAPYDV
                     VLRGGRVIDGTSAPWYIADVAIRDGKIARIGRIDAAGAKRSIDATGLVVAPGFVDMMG
                     QTATPMLDAPHSAMNLLTQGITTINAGEGVSAAPLDEDEARRMGWRSMADYFQVLEKK
                     GLPLNVAQTVGHTQVRQIVLGDADRRPSEEELERMADLVREAMESGAIGVSTALIYPP
                     AVYARTEEIGRLAEIAGQYGGRYYTHMRNEGDQLEEAIDEALAIGRRGKTPVHIFHLK
                     AAGRQNWGKMRLAIAKIQAARQAGQQVTADIYPYVNNGLGIAAFIHPRHSASGAGSLI
                     KKLDDPAARDEIRKEMETTGGWENWYRHVGHDWNKVVIGQANEPRYAPHAGKTIGEIA
                     EACGEAPWDTFFQLVRTGAFALPESMTEANKIIAMQQPFVSFCTDVGPAGVGRIASHP
                     RAFGSFPRLLSHYVRDLGAIPLERAVAQASAAGANSVLA"
BASE COUNT          439 a          698 c          717 g          400 t
ORIGIN      
        1 cccgcagcgg caatggcccg gactcggcgc ataatccctg cgccgggtgc tcgggcgcga
       61 accctgtctc aagtctaact gcctctcagc gggccagcaa gcaagaagtc ctgaacttag
      121 cccagcttag gtttcgtcca acaattccgc ccgtcgggta atacttgcct ccggctcaat
      181 cccattcgct ttgccccccc cgacacgtac tcggtaccca gtatgaggcc cctcgaccgc
      241 cgggccacac cggctagaat ctaacgaaga cgtgcggcag ttgtactcgc cgtttccaat
      301 tgacgcgggg tggagcagcc cggtagctcg cgaggctcat aacctcgagg tcgcaggttc
      361 aaatcctgtc cccgcaactt gaattgacag tgtcagaaat atggcgacta acggcttttt
      421 cgagaggccg acgtccgcag acgtcggcct cgttcgttta agggcccgct gtggcgacgg
      481 cttgcggcgg ccgagcgagc gtattctccg ggcacgagcg gtcagcaatt cctactttta
      541 ccttttcagt cgtgaagttt gacggttttt acttctcgaa acctgtggtg cagtgagtcc
      601 agccgcggga ggaccagccc tatcccattc gtcgcgccgc gggctacagc agtggcttag
      661 ccgacgagtc gaaatggcga cgaatgcctg cgcgccatcg tcagcaacag tcgcatctct
      721 tacactgacc aagtcagcgg tgatgtattc tcgcagacat gtggcgaatt gccgagagag
      781 gtgccaatgg cgcgggaaat caatcagccg tcacttgcgc ctgcagtcag ccgcatgccg
      841 cgaatcgtgt tttcttccgc cgcgctactc gtcgcgctgg cagcttcccc gttactggcc
      901 gaagcaccgt acgacgtggt cctgcgcggc ggacgtgtga tcgatgggac cagtgcgccg
      961 tggtatatcg ccgatgtggc aattcgtgac ggcaagatcg ctcgcatcgg ccggatcgac
     1021 gccgcgggcg cgaagcgctc aattgacgcc acgggcctgg ttgtcgcgcc aggctttgtg
     1081 gacatgatgg ggcagacggc cacgccgatg ctcgacgccc cgcattcagc aatgaacctc
     1141 ctgacgcagg gcatcacgac aatcaacgcc ggagaggggg tctcggccgc tccgctcgat
     1201 gaggacgagg cccgccgcat gggctggcgg tcgatggctg attattttca agtgcttgag
     1261 aaaaaggggc tgccgctgaa cgtggcccag acggtcggcc acacgcaggt gcggcagatc
     1321 gttctgggcg atgccgatcg ccggccgagc gaggaagaac tcgagcggat ggcggacctg
     1381 gtgcgcgagg cgatggagtc aggagcgatc ggcgtttcga cggcactgat ctatccgcca
     1441 gccgtctatg cccgaacgga ggagatcggc cggctggcgg aaatcgccgg gcaatatggt
     1501 ggccggtatt acacacacat gcggaatgaa ggggaccagc tcgaggaggc gatcgacgag
     1561 gccctggcga tcggccgccg cggtaaaacg cccgttcaca tctttcacct caaggccgcc
     1621 gggcgccaga actggggcaa gatgcggctg gcgatcgcga aaattcaggc cgcacgtcaa
     1681 gcggggcaac aggtaaccgc cgacatctat ccgtacgtga acaacggcct ggggatcgcc
     1741 gcgttcattc atccgcgcca ctcggcgagc ggcgctgggt cgctcatcaa gaagctcgac
     1801 gacccggccg cgcgggatga aatccgcaaa gagatggaaa ctaccggcgg ctgggagaac
     1861 tggtatcggc acgtcggcca cgactggaac aaagtcgtca tcggacaggc gaacgagccg
     1921 cgctacgcgc cgcacgccgg caaaaccata ggcgaaattg ccgaagcctg cggcgaagcc
     1981 ccctgggaca cattctttca gcttgttcgc acgggagcct tcgccctgcc cgagagcatg
     2041 accgaggcaa acaagatcat cgcgatgcag cagccattcg tttcgttctg taccgacgtg
     2101 ggccccgcgg gggtcggccg gattgcgtcg cacccgcggg cgttcggctc gtttccgcgt
     2161 ctgctgtccc actacgtccg agatctaggg gcgatcccgc tggagcgggc ggtggcgcag
     2221 gctagcgcag caggagcaaa cagcgtactg gcct
//
LOCUS       DASUGB010000030         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1670690, whole genome shotgun sequence.
ACCESSION   DASUGB010000030 DASUGB010000000
VERSION     DASUGB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670690"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFV87_00590"
     CDS             <1..249
                     /locus_tag="VFV87_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102275.1"
                     /translation="CETLEERLLAAACRYSACPHIALLSPAPPGAGWRKLAQKFRKKW
                     VHLPLGQFSDATVQQLRMVHVLNGRQVRSYAADFIRKA"
     gene            459..917
                     /locus_tag="VFV87_00595"
     CDS             459..917
                     /locus_tag="VFV87_00595"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEX5102276.1"
                     /translation="MPKPGPEHAYLKAMEGVWDAEMVTPDGNKSAGTMTYKMQVGGLW
                     LTSDYKGEFAGAEFTGKGLDSYDAKKKKYVGVWVDSMITSPLIMEGDMDEKTHTLTMN
                     TEGPGPDGKPQKMKMVTKMPDKDHMNFEMFMTGADGKETSGFKINYTRKK"
     gene            complement(1059..2078)
                     /locus_tag="VFV87_00600"
     CDS             complement(1059..2078)
                     /locus_tag="VFV87_00600"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5102277.1"
                     /translation="MNSLRIVSALVLFIVAIQRSQAQMPDLRNTPPPAALLAPQAKVT
                     MAAGVCFLEGPAAGADGTVYFSDISGNRILKMDAEGKISTFRADSGRTNGNTFDARGR
                     LISCEGAENGPGGRRRVVRTDLTTGKIEILTERYEGKRYNSPNDVVVDPAGRIWFTDP
                     LYAPDRSIMEHEHEAVYRIDTDGKVTRVITQPAIGRPNGLAITPDGKTLYVVDSNYIN
                     PDANRKIWAFDIAADGSASKQRQVYDFGRGRGGDGMRLDAKGNLWIAAGVSAPRSANE
                     SGDVPTGVYVISPQGELLGRIPIPEDVITNLAFGGPDKKTLYVTAGKTIFKIPTAVSG
                     YSLYP"
     gene            complement(2075..>2432)
                     /locus_tag="VFV87_00605"
     CDS             complement(2075..>2432)
                     /locus_tag="VFV87_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5102278.1"
                     /translation="GLDLTFSCEAAEPNLSPAARLMLLRIAQEALNNAWKHARASRVQ
                     MQLSDVDGALELAIADSGPGFNVQSRPLHFGLDGMRARAHILGADLNIQSRAGEGTRI
                     AVRLALPQPTGREHPT"
BASE COUNT          438 a          701 c          844 g          449 t
ORIGIN      
        1 tgcgagacgc tcgaagagcg gctgctggcg gcggcctgcc gctatagcgc ctgcccgcac
       61 atcgccctgc tctcccctgc cccgcccggc gcgggctggc ggaagctggc ccagaagttc
      121 cgcaagaagt gggtccatct gccgctgggc cagttcagcg atgcgacggt gcagcaactc
      181 cgcatggtcc acgtgctcaa cggcaggcag gtgcgaagtt acgccgccga tttcattcgc
      241 aaggcttaat ggggcgtgtt taccggtgta cgccagatga attccgcgct gtggcagatt
      301 gacagaatct gcagcgcgcg ttctggtgag acgcgaggga tgggttctgc ttatcacacg
      361 ttcctggaga ttctctcatg tctcgtctgg ttgctttggc gttggtctgt gccgctgcgg
      421 taatggcgcc caggaccctt cgggctcagg agtttgaatt gcccaaaccg ggccccgagc
      481 atgcttactt gaaggcgatg gagggggtgt gggacgcgga aatggtgacg cccgacggca
      541 acaaatcggc ggggacgatg acttataaaa tgcaggtcgg cggattgtgg ctcaccagcg
      601 attacaaggg ggaattcgcc ggggcggagt tcaccggcaa ggggctcgac agctacgacg
      661 cgaagaagaa aaagtacgtc ggcgtgtggg tcgattcgat gatcacctcg ccgctgatca
      721 tggaaggcga catggatgag aaaacgcaca cgctgacgat gaacaccgaa ggcccgggcc
      781 cggacggcaa gccgcagaag atgaagatgg tgaccaagat gccggacaag gatcacatga
      841 acttcgagat gttcatgacg ggcgcggacg gcaaagagac gagcggcttc aagatcaatt
      901 acacgcggaa gaagtagatc gtagatggta gggtgggacc agggagcgaa gcgagcgcag
      961 gcccaccacg cactggcgac cactggtggg cctgcggccg cggcggcctt gtcccaccta
     1021 cattctgatc gtccattgcg tctcgcagag cgagacgcct acggataaag ggagtagccg
     1081 ctgacggcgg tggggatctt gaagatcgtc ttgccggcgg tgacgtagag cgttttcttg
     1141 tcgggtcccc cgaaggcgag gttcgtgatc acgtcctcgg gaatgggaat gcgtcccagc
     1201 aactcgccct ggggcgaaat gacatagacg ccggtcggca cgtcgccgga ttcgttggcg
     1261 gagcgcggcg cggaaacgcc cgccgcgatc cacaggttgc ccttggcatc gagccgcatg
     1321 ccgtcgccgc cgcggccgcg gccgaagtca tagacctgcc gctgcttgct cgcggagcca
     1381 tcggcggcga tgtcgaaggc ccagatcttg cggttagcat cggggttgat gtagttgcta
     1441 tcgacgacgt acagcgtctt gccatcgggc gtgatcgcca gcccgttggg ccggccgata
     1501 gcgggctgcg tgatgacgcg ggtcaccttg ccgtcggtat cgatgcggta caccgcttcg
     1561 tgctcgtgct ccatgatcga gcgatcgggg gcgtagagcg gatcggtgaa ccagatgcgg
     1621 ccggctggat cgacgacgac atcgttggga ctgttgtagc gtttgccttc gtagcgctcg
     1681 gtgaggattt cgattttgcc agtcgtcaga tcggtgcgga cgacccgccg ccggccgcca
     1741 ggtccgtttt cggccccttc gcaagagatc aggcgacccc gggcgtcgaa cgtgttgccg
     1801 ttggtgcggc cgctgtcggc ccgaaaagta gagatcttgc cttccgcatc cattttgagg
     1861 atccggttgc cgctgatgtc gctgaaatag accgtgccgt cggcgccggc ggcggggcct
     1921 tccaggaagc agacgcccgc ggccatagtg accttggcct ggggcgccag gagcgccgcg
     1981 ggcggcgggg tgtttcgcag atcgggcatt tgcgcctggc tcctttggat ggcgacaata
     2041 aacaggacga gagccgatac gattcgaagg ctgttcatgt cggatgctcc ctgccggtgg
     2101 gttgcggcag ggcgagccgc acggcaatgc gggttccctc ccctgccctg ctctggatgt
     2161 tcagatcggc gcccaggatg tgggcccggg cccgcatgcc gtccaggccg aagtgcagcg
     2221 gccgcgactg cacgttgaaa cctggaccac tgtcggcgat tgccaattcc agcgctccgt
     2281 ccacgtcgga gagctgcatc tggacgcggc tggcgcgggc gtgcttccag gcgttgttga
     2341 gcgcctcctg ggcgatccgc aggagcatca gccgggcggc tggcgagagg ttgggttcgg
     2401 ccgcctcgca cgaaaaagtc aaatcgagcc ca
//
LOCUS       DASUGB010000031         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_698036, whole genome shotgun sequence.
ACCESSION   DASUGB010000031 DASUGB010000000
VERSION     DASUGB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698036"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFV87_00610"
     CDS             complement(<1..260)
                     /locus_tag="VFV87_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5102279.1"
                     /translation="MRWSIRLGRVLGIDIYVHLTFLLLLAWVVISAYNQTHSVARALF
                     ELAFICLVFATVVMHEYGHALTARQFGVKTRDITLLPIGGVAR"
     gene            556..1488
                     /locus_tag="VFV87_00615"
     CDS             556..1488
                     /locus_tag="VFV87_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5102280.1"
                     /translation="MAPAVCGEISKITPTFKKAGDLEIKADVYQPANAARDGKRPVVV
                     WIHGGALINGHRESVPKWLLEGSLAQGWNVVSIDYRLAPETQLPEIVADLDDAFRWVR
                     EKGPELFAADPERVAAIGGSAGGYMTLTAGFRAKPKPVALVSLWGYGDLVGPWYSEPS
                     PHPRHRTSQFSREEAYKQVSGPPISDSRDRQGDGGAFYQYCRQTGTWPKAVSGWDPHK
                     EPEKFHPYMALKNVTADYPPTLLIHGDQDTDVPYEQSTLMAAELKKHGVEHQLITLKG
                     AEHGLAGGGKAEIDAAYAAAIEFLKKHLAPGPSK"
     gene            1485..2951
                     /locus_tag="VFV87_00620"
     CDS             1485..2951
                     /locus_tag="VFV87_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102281.1"
                     /translation="MSTTASATSSPAAQRPPSVWRRMRWTIRTLLLVVTLIALLLAWI
                     GRTIRAVHHRQVLATALESDDGIDTYLRWGSFRDSRLEPNWLAIFCGDWSSVELTDVD
                     IDYRQTASDDLLEQIGVFRKLEDVSISGQQVTDRGLAPLGHMQELRSLTIEACDITDK
                     TLEAIADLPNLQDVTLRGVAVTDQGLAHLWKLPNLTSLTLDFTLVTPKAVKAFQNGRP
                     DCVVGYTPAAGKPQVEAAKGIMRRDANLLYDADTRLITAMIFDMGHKPWRSSDWALLH
                     AIPELQAVSIDDLPSAAEGLREVQKLPNVEGLILDSVPVTPGELLVLTEYPKLTTLHF
                     QPENLTDQHLVALGKLTNLRVLKIEPEAATISRRGIEALAGLKQLRHLKLSSVPILDE
                     DLEPLAELPMLTTFDLSMTPVTDKALDYLLGMPQLRELILDETQITGAGAQRIMKSGH
                     ILSATWAETGIKPEFFGRPPPPATTGFEPPLIVPPYPR"
     gene            3063..3266
                     /gene="rpmI"
                     /locus_tag="VFV87_00625"
     CDS             3063..3266
                     /gene="rpmI"
                     /locus_tag="VFV87_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5102282.1"
                     /translation="MPKQKTHKGTKKRFRLTASGKAKHRQAGTSHLASRMSKKRRRNL
                     RGTKVVSDTVIHQITRALAGHSS"
     gene            3464..3820
                     /gene="rplT"
                     /locus_tag="VFV87_00630"
     CDS             3464..3820
                     /gene="rplT"
                     /locus_tag="VFV87_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5102283.1"
                     /translation="MRATKGAARAKAKRRIFKRAEGFFGGRRKLLRTAKETLLRAGKF
                     AYRDRRVRKRDFRRLWITRLSAACRERDLRYSQLIAGLKAANIELDRKSLSEIAIADP
                     VAFDAIVAEVKKALKL"
     gene            3905..>4203
                     /locus_tag="VFV87_00635"
     CDS             3905..>4203
                     /locus_tag="VFV87_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5102284.1"
                     /translation="MALQDFVADLDSLLADATRAFEGAGDAAALEAARIEFLGAAKGR
                     LKGVQKGLGAVAKEDKPAAGKRLNEVKAAVEAAFEAGQTRLKSAGPAKKAGPQFDP"
BASE COUNT          822 a         1324 c         1356 g          701 t
ORIGIN      
        1 cgggccacgc cgccgatcgg cagcagcgtg atgtcgcgcg ttttgacgcc gaattgccgc
       61 gcggtcaggg catggccgta ttcatgcatc accaccgtcg cgaaaaccag gcagatgaac
      121 gccagctcaa acagcgcccg cgccacgctg tgtgtctggt tgtacgccga gatcaccacc
      181 cacgccagca agagtaagaa cgtgagatgg acgtagatat cgatgccgag tactcggcct
      241 aaccgaatcg accagcgcat gatgctcctt acttattcgc tcgcggcagc caattctccc
      301 agaatggcat gaatgcgcga accggccgcg ttgaagatgc aaagcacgtg ccgacgatgg
      361 cctgcagatt ggtttgcgag cttcattggt ttgggtctgg tttgcagggt actttacgat
      421 cggagacaac cttggcgact ggatggagat acaatgacca attcgggtgg acgatcgact
      481 ggggaggtgt caatgattgg agatttctgt ggcagacttg cggctacgct cagcttcctg
      541 gcgggagtga ttgctgtggc tcccgccgtc tgcggcgaga tcagcaaaat tacacctacc
      601 ttcaagaaag ccggcgacct ggagatcaag gccgacgtct atcaaccggc gaatgccgca
      661 cgggacggca agcggccggt ggtggtgtgg attcacggcg gggcgttgat taacggacat
      721 cgcgagagcg tgccgaagtg gctgctggag gggtcgctgg ctcagggctg gaacgtggtc
      781 tcgatcgact atcggctcgc gccggaaacg cagttgccgg aaatcgtcgc cgatctggac
      841 gacgccttcc gctgggtgcg ggagaaaggg ccggagctgt ttgctgccga tccggagcgg
      901 gtcgctgcta ttggcggatc ggcgggcgga tatatgacgc tgacggctgg ctttcgggca
      961 aaaccaaaac ccgtggcgct cgtctcactg tggggatacg gcgacctcgt cggaccgtgg
     1021 tacagcgagc cgagcccgca cccgcggcat cgcaccagcc agttcagccg cgaggaagca
     1081 tacaagcagg tttccggccc gccgatctcc gactcgcgcg accgccaagg ggacggcggc
     1141 gcgttttacc aatactgccg ccagaccggc acgtggccca aagcggtcag tggctgggac
     1201 ccgcacaagg agccggagaa gttccatccc tacatggccc tgaaaaacgt gactgccgac
     1261 tatccgccga cgctcctcat tcatggcgac caggacaccg acgtgccgta cgagcaatcg
     1321 acactcatgg ctgccgagct gaagaagcac ggcgtggagc accagctcat cacactgaag
     1381 ggcgcggagc acggcctggc cggcggcggc aaggctgaaa tcgacgcggc gtatgcggcg
     1441 gcgatcgagt tcctgaaaaa gcacctggcg ccagggccct cgaaatgagc acgaccgctt
     1501 ccgcgacctc cagccctgcc gcgcagcgtc cgccgtccgt ctggcggcgg atgcggtgga
     1561 cgatccgaac gctgctgctg gtggtgacgc tgattgctct actcctggcg tggattgggc
     1621 ggacgatccg ggcggttcat caccggcaag tgctggcaac ggctttggag tcggacgatg
     1681 gcattgatac atacctccgc tggggttcgt ttcgtgacag tcgtcttgaa cccaactggc
     1741 tcgccatctt ctgcggcgac tggagcagcg tcgaattgac cgatgtcgat atcgactacc
     1801 ggcaaacggc cagcgacgac ctgctggagc aaatcggcgt ctttcggaaa ctggaggacg
     1861 tttcgatcag cggccagcag gtcaccgacc gcggtctcgc gccgctgggt cacatgcagg
     1921 aattgcggtc gctgacgatc gaggcctgcg acatcaccga caagacgctg gaggccatcg
     1981 ccgacctgcc caacctgcaa gacgtcacgc tgcggggcgt ggcggtcact gaccaggggc
     2041 tggcccatct gtggaagctg cccaatctca cgtcgctcac gctcgacttc acactcgtca
     2101 cgccgaaggc agtgaaagcc tttcagaacg ggcggcccga ctgcgtcgtc ggctacaccc
     2161 ccgccgccgg caagccgcaa gtcgaagccg ccaagggaat catgcgccgc gacgccaacc
     2221 tcctgtacga cgccgacacg cggctgatta cggccatgat cttcgacatg ggccacaagc
     2281 cgtggcgctc gtcggactgg gcgttgctcc acgcaattcc cgagctccag gccgtttcga
     2341 tcgacgatct gccaagcgcc gccgaaggcc tgcgcgaggt gcagaagctc ccgaacgtgg
     2401 agggactgat cctggattcc gtcccggtca ctcccggcga attgttagtc ctgacggagt
     2461 accccaagct tacgaccctg catttccagc cggagaacct gaccgaccag cacctggtgg
     2521 cgctcggcaa gttgacgaac ttgcgggtgc tgaagatcga gccggaggcc gccacgatct
     2581 cgcgacgcgg catcgaggcg ctggccgggc tcaagcagtt gcggcaccta aagctcagca
     2641 gcgtgccgat cctcgatgag gacctggagc cgctcgccga attgccgatg ctgacgacgt
     2701 tcgacctctc gatgacgccg gtgaccgaca aggctctgga ttacctgctg gggatgccgc
     2761 agcttcgcga gctgattctg gacgagacgc aaatcaccgg tgccggcgcc cagcggatta
     2821 tgaaatccgg gcacattctg tcggcgacct gggccgaaac gggcattaag ccggagtttt
     2881 tcggccgtcc gccgccccct gcgaccaccg gtttcgagcc gccgctgatt gtgccgccat
     2941 atccgcgata ggcggctgct tcggcagggc aaaatcgccc cttttgccga cgggaccaag
     3001 cccctatact acagggcttt ccgcaactgc agatttcgcc cagcgcgccc ggggggccat
     3061 ttatgcccaa gcaaaagaca cacaaaggga ccaagaagcg tttccgcctg acggccagcg
     3121 gcaaggccaa gcaccgccag gcggggacga gccacctggc ctcgcggatg agcaagaagc
     3181 gccgccgcaa cctgcggggg acgaaggtcg tcagcgacac ggtgatccac cagatcacgc
     3241 gggccctggc gggacacagc agctagccgc aggacgggtt ttcaacccgt cctgccgtag
     3301 gatgggtttc caactcgtcc agagtaggac gtggtttcca acccgtccgt acgagtcagg
     3361 cgggttaaga aacccgccct acgaacacac aagtacgcgg gcgcctaacg cccgagccgg
     3421 tcgctcgggg gccttgccgt gccagatttg aaggacgcat tccatgcgtg caaccaaagg
     3481 cgccgcccgg gccaaagcca agcggcggat tttcaagcgg gctgagggct ttttcggcgg
     3541 acggcgcaag ctgctccgca cggccaagga aacgttgctg cgggcgggta aatttgccta
     3601 ccgcgatcgc cgcgtccgca agcgcgattt ccgccgcctg tggatcaccc gcctcagtgc
     3661 cgcctgccgc gagcgcgacc ttcgctacag ccagctcatc gccgggctga aggcggccaa
     3721 catcgagctc gaccgcaaga gcctctcgga gatcgccatc gccgacccgg tcgccttcga
     3781 cgcgatcgtc gccgaggtga aaaaggctct caagctgtaa agtctgcctt ttgccgagac
     3841 caaactggtc ccggcgagtc ggctgaccga accgattcac ggatgcgccg gtccttctgc
     3901 tgccatggca ctgcaggatt ttgttgccga tctcgattcg ctcctggcgg acgcgacgcg
     3961 cgcgtttgaa ggcgccggcg atgccgccgc gctcgaagcc gcgcggatcg agttcctcgg
     4021 cgcggccaaa ggccggctga agggggtgca gaaggggctc ggagcggtcg ccaaggaaga
     4081 caagcccgcg gcgggcaagc ggctgaatga ggtgaaggcc gcggtggaag ccgcgttcga
     4141 agcgggccaa acgcggctga agtctgccgg tccagccaag aaggcggggc cgcagttcga
     4201 tcc
//
LOCUS       DASUGB010000032         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_698390, whole genome shotgun sequence.
ACCESSION   DASUGB010000032 DASUGB010000000
VERSION     DASUGB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698390"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFV87_00640"
     CDS             complement(<1..1037)
                     /locus_tag="VFV87_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5102285.1"
                     /translation="MTTKLLPPQSDPAPIFEIFRGNFAMELLTAAVAHFGVFDTLAAG
                     PIAPDDLRKKIGLGERQFIVLMTGLKALKLVEERGGKLQATPLAREHLTPGGPLDVSD
                     YIRMGADAPGVLSLVQHMQTSKLAGAAKDDDRAVFIFKDGLESAMHADDLARHFTLML
                     AGRAKNIAPVLAQKVDLSDAKCLLDIGGGTGLYSIAFLQRFPKLKAIILDSPAVLKVA
                     AEFAQRYGVADRLTCQPGDMFKDPLPTRCEVILLSNVLHDWDVPECEQLVAKAAAALP
                     AGGRLLIHDAFLSDDHSGPLYPALFSVALMIMTEGRNYSGAEYAAWMRSAGLTPAQPI
                     PTRVRSSVQVGP"
     gene            1266..2456
                     /locus_tag="VFV87_00645"
     CDS             1266..2456
                     /locus_tag="VFV87_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX5102286.1"
                     /translation="MRYEHIVSDQHLRDFCQTIAAAPILAFDTEFVSEDSYQPELCLI
                     QLAADGQLAIVDPLEVDLAPLWDLLVQAGRQTLVHAGREEFRFIRKHTGQRPASWFDV
                     QIAAGLIGMEYPASYGTLSAKLLGRVLPKDETRTDWRRRPLTQRQLEYALQDVAELDQ
                     IRTALHRRLSELGRLEWLEAELADWQRELEEYEDGERWQRTAGIAGLSLRSLAIVREL
                     WHWRDAEAQRRNVPTRRVLRDDLLIEIAKRQTADPKRIRAVRGMERGDLQRYLPKIGQ
                     CVEEGLALKESELPRLPRKSSRPQLNLLGQFLSTALGSICRRAEVAPSLAGTAQDVRD
                     LIAYRLNLGGFAPGEVPLLARGWRAEVVGQAIDHLLAGDLSIRIGDPLSDEPLEFERK
                     NRSP"
     gene            complement(2540..3598)
                     /locus_tag="VFV87_00650"
     CDS             complement(2540..3598)
                     /locus_tag="VFV87_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5102287.1"
                     /translation="MPTLAPSQLEVFARQLLAAGGASADEAQIVAASLVDANLKGYDS
                     HGVMRIPYYVQAIKEGEVAPGEQLTILDEGPSRVVADANWGFGQVQAVRLLKMLASRA
                     HSEGLAIGTMTHSGHIGRLGEYCEMAAAEGLASMLMVNSHGAAVRVAPPGGKAPRLST
                     NPLAIGVPNGASPLVLDFSTSATAEGKVRVKKIAGQQCPEGWLIDSDGKPTCDPNSLY
                     GNPPGSILPMGGAQAYKGFGLGIMIEILTGALSGGVCARPAPYPKKGNCVFILLIDPA
                     RFGGAEHFQAEVAQLSDYVKSCPRVEGCDEILLPGDPERRLAATRRQEGITLDDENWK
                     ALVKLANSLGVFAPATAS"
     gene            complement(3684..>3932)
                     /locus_tag="VFV87_00655"
     CDS             complement(3684..>3932)
                     /locus_tag="VFV87_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEX5102288.1"
                     /translation="LIFHRGPLTGGTELTRQFALRHGKPLLSFDLNDKPKPANILQWI
                     KLHSIQSLNIAGPRESTCPGIYEQARGLLVTLLSSRDR"
BASE COUNT          692 a         1175 c         1368 g          697 t
ORIGIN      
        1 ggtcccacct gcacgctgct gcgcacgcgg gttgggatgg gctgggcggg ggtgaggccg
       61 gcggagcgca tccaggcggc gtattccgcg ccgctgtagt tgcggccttc ggtcataatc
      121 atcagggcta cggagaacag ggcgggatag agcgggccgg aatggtcgtc gctgaggaag
      181 gcgtcgtgaa tgagcaatcg gccaccggcg gggagcgcag cggcggcttt ggcgacgagc
      241 tgctcgcact ccggcacatc ccagtcgtgc agcacgttgg aaagcaatat cacctcgcag
      301 cgtgtgggga gtgggtcttt gaacatgtcg cccggctgac acgtgaggcg gtctgcgacg
      361 ccgtatcgct gggcaaattc cgcggcgact ttcaggacgg cagggctgtc gagaatgatg
      421 gccttcagtt tgggaaaccg ctgcaggaag gcaatgctgt aaaggccagt accgccgccg
      481 atgtcgagca ggcacttggc atccgagaga tcgactttct gcgccagcac cggggcgatg
      541 tttttggccc ggccggcgag catcagcgtg aagtggcggg ccagatcgtc ggcgtgcatg
      601 gccgattcca gtccgtcctt gaagatgaag acggcgcggt cgtcgtcctt ggccgctccg
      661 gcgagcttgc tcgtctgcat gtgctggacg agcgacagca cgccgggcgc atcggccccc
      721 atgcggatgt agtcgctcac gtccagcggt ccgcccggcg taaggtgctc gcgtgcgaga
      781 ggcgtcgcct gcaacttccc gccgcgctcc tcgaccagct tgagggcctt gaggccggtc
      841 atgaggacga tgaattgccg ctcgcccagg ccaatcttct tccgcagatc gtccggcgct
      901 atgggccccg ccgcgagtgt gtcgaagacc ccgaaatgag ccacagccgc ggtgagcagc
      961 tccattgcaa aatttccgcg gaaaatctcg aagatggggg ctgggtcgga ttgtggcggg
     1021 aggagcttgg tggtcattgg caggggacag gggacaggag tcaggagtca ggagacagga
     1081 gtcaggagac agataatcgt atcgcaattt gctgatgttc tggggtggag tcggctggcc
     1141 aaaatgcggg ccgtcgcgta agattccgcg cgtttgcgct gcttactcct cgtattgcct
     1201 ctttcgtccg gcagccaccg tcagctccct cctcgcggca tgccggccgg cgttgctttt
     1261 cgcccgtgcg ttacgaacat atcgtttccg accagcattt gcgggatttc tgccagacga
     1321 tcgcggccgc gccgatcttg gctttcgata cggagttcgt ctcggaagac tcctatcagc
     1381 cggagctgtg cctgatccag ctggcggctg acggtcagtt ggcgatcgtc gatccgctgg
     1441 aggttgatct tgcaccgctg tgggatttgc tggtccaggc ggggcgccag acgctggtcc
     1501 acgcggggcg ggaggagttc cgcttcatcc gcaagcacac gggccagcgg ccggcgagct
     1561 ggttcgacgt gcagatcgcc gccggactga tcggcatgga gtatccagcg tcgtacggga
     1621 cgctctcagc gaaattgctc ggccgcgtgc tgcccaagga cgaaacgcgg accgactggc
     1681 ggcggcgacc gctcacgcag cggcagcttg agtatgccct gcaagacgtg gcggagctgg
     1741 atcagattcg caccgcgctg catcgccgac tgagtgagct gggacggctc gaatggctgg
     1801 aagcggagct ggccgactgg cagcgcgagc tggaagaata cgaagacggc gagcgctggc
     1861 agcggacggc aggcatcgcg gggctctcgc tccgcagcct ggcgatcgtc cgcgaactgt
     1921 ggcactggcg ggacgccgaa gcccagcggc ggaatgtgcc cacgcggcgc gtccttcgcg
     1981 acgacctgct catcgaaatc gccaaacggc agacggcgga tcccaagcgg attcgggccg
     2041 tccgcggcat ggaacgcggc gatctgcaac ggtatctgcc gaagattggt caatgcgtag
     2101 aagaaggtct agcactgaag gaatccgaat tgccgcggct gccgcgaaag tcgtcgcggc
     2161 cacagctcaa cctgctcggc caattcctga gcacggccct gggcagcatc tgccgccgcg
     2221 cggaagtcgc gccgagcctg gcgggcaccg cgcaggacgt gcgcgacctg attgcctatc
     2281 ggctgaatct cggcggcttt gctcccggcg aagtgccgct cttggctcgc ggctggcggg
     2341 cggaagtggt cgggcaagcg atcgaccatc tgctggcggg agacttgtca attcggatcg
     2401 gcgacccgct gtctgacgag ccgctggagt ttgagcgaaa gaatcgtagc ccgtagcgca
     2461 agcgagggtc aacgatgtca acttgcaatt ccgcgcggat cgatgaatca acatccattt
     2521 ccctcgcttg cgctacgggc tacgatgcgg tcgctggcgc gaatacaccc aaactgttcg
     2581 ccagcttcac cagcgccttc cagttttcat cgtccagcgt gatgccctcc tgccggcgcg
     2641 tggcggccag tcgccgctcg gggtcgccgg ggaggaggat ttcgtcgcag ccttcgacgc
     2701 gcgggcacga cttgacgtag tcgctgagct gcgcgacctc ggcttgaaaa tgttccgcgc
     2761 cgccgaagcg ggcggggtcg atcagcaaga tgaaaacgca gttgcctttc ttcggatacg
     2821 gcgccgggcg ggcgcacacg ccgccggaga gggcgccggt gaggatctcg atcatgattc
     2881 ccaggccaaa gcccttgtag gcttgcgcgc cgcccatcgg cagaatcgag ccgggcggat
     2941 tgccgtagag tgaattcgga tcacacgtcg gcttgccgtc gctgtcgatg agccagccct
     3001 cgggacactg ctggccggca attttcttaa cacggacttt gccctccgcc gtggcgctgg
     3061 tgctgaagtc gaggacgagc gggctcgcac cattgggcac gccgatcgcc agcggattcg
     3121 tcgaaagccg cggggccttg ccgccaggcg gagcgacgcg gaccgcggcc ccgtgcgaat
     3181 tgaccatcag catcgaggcg agcccctcgg ctgccgccat ctcgcagtat tcgcccagcc
     3241 ggccgatgtg cccgctgtgg gtcatcgtgc cgatcgccag cccctcggaa tgcgcccgcg
     3301 aggccagcat cttgagcagc cgcacggctt gaacctggcc gaagccccag ttggcgtcgg
     3361 ccacgacgcg cgacggacct tcgtcgagga tcgtcaactg ctcgcccggg gcgacctcgc
     3421 cttccttgat cgcctggacg taatacggaa tccgcatcac gccgtgcgag tcgtagcctt
     3481 tcagattggc atcgaccagg ctcgcggcga cgatctgggc ctcatcggcg ctcgcgccgc
     3541 cggctgcgag gagttggcgg gcgaagactt cgagctggga gggggcaagg gtaggcatgg
     3601 gaggaggaca gaggacagag gacagaggac aacgacagtg gacagagtaa acgagaggtt
     3661 cggtcggacc aagacctcgg cacttatcga tcacgactgc taaggagggt aacgagcagg
     3721 ccacgagctt gttcgtagat gccggggcag gtgctctcgc gcgggccggc gatgttgagc
     3781 gattggatgg aatgcagctt gatccactgc aaaatgtttg cgggcttagg tttgtcgttc
     3841 aagtcgaagg aaagtagcgg cttgccgtga cgcagggcga attggcgcgt cagctcggtg
     3901 ccgccggtca gggggccgcg atggaagatc ag
//
LOCUS       DASUGB010000033         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_144010, whole genome shotgun sequence.
ACCESSION   DASUGB010000033 DASUGB010000000
VERSION     DASUGB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144010"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..780)
                     /locus_tag="VFV87_00660"
     CDS             complement(454..780)
                     /locus_tag="VFV87_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6793 family protein"
                     /protein_id="HEX5102289.1"
                     /translation="MPLFEVETDAHIIITWAADETAAGGVVREAYPSDKVIRMTKRPR
                     DTWVISKGALGITNTKMDVCGTARDCLSKASGDKVHAIRLYMHETGTDLEHARKVIES
                     NMVMGW"
     gene            1024..2280
                     /locus_tag="VFV87_00665"
     CDS             1024..2280
                     /locus_tag="VFV87_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102290.1"
                     /translation="MAIDTPAKIAVLGAGPIGLEAALYARFLGYEVVVFEAGEVAASV
                     RRWGHVRMFTPFGMNRSPLGLAAIQAQDEGYRPPGDDELLTGHEWVDRYLLPLSQTDL
                     LADHLRLQTNVLRVGKERLLKGESPGHAERGDFAFRLLVRDSDGNERIETADAVIDAS
                     GTFGLANHLGHGGIPAPGEAACADKIEWRLPDILGRDREHYAGHHTLLIGAGYSVATN
                     AVALSRLIAEVPGTRVTWITRREPAAGAAGPIEEIHDDRLPERAGLARRANELANDPN
                     SGINYWPATEVERIEGEFSVELSGRHAGIYSLDRIIANVGFRPNTQIYEELQVHECYA
                     TQGPMKLAAAMMGQQSADCLDQKACGPQSLLNPEPNFYILGAKSYGRKSNFLMSVGLE
                     QIREIFTILGDRPTLDLYAGAHNLLR"
     gene            2284..3192
                     /locus_tag="VFV87_00670"
     CDS             2284..3192
                     /locus_tag="VFV87_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5102291.1"
                     /translation="MPQTTDLPLVSISHLDHLWFQVGGTLCNLTCRHCFISCSPKNDS
                     LGFLSLEQVKSRLEESVPLGVKEYYFTGGEPFLNPEMTEILVETLRYGPATVLTNGTV
                     LKDEWLTRLRTAEDAGPYSLEFRVSIDGFSPETNDPIRGEGTFARALKGIQKLVQHGF
                     LPIVTAAQVWPDEQNHDFVERFVKVLKAAGYSRPRLKILPMLRLGAEECRTHDYLASE
                     RVDRAMLEGYDTSQLVCEHSRCVTDRGVHVCPILIESPDSLLGQSLNVSLQPFAIRHG
                     ACYTCYQHGAICSNPSSLPARQEAPA"
BASE COUNT          622 a         1012 c         1014 g          599 t
ORIGIN      
        1 ctgcctcggc cggttcggcg gtgccgctag cggcgggatc ttccgctact tcgacggcgt
       61 ttgtcggctg ctgggcaatt gcgccaggtg cgcaccaggc cagggcggcc agggccggaa
      121 gagtgcaaca ccacgaacga acggtgattt tcatcgcgca ctcctttgcg ctgcggactg
      181 gcaggttgcg gctggcggga cgacgcgcgg gtgcgagtta tcttcaagcc ggggttcgta
      241 tggcggcggg tgggacgcct acgaaaggca acggcaggga aaagtgccgc ttcgggcgct
      301 acgaacccac agatcgtttt cgacggtcgg cgtccaaatt tagagcatca cggcgacaac
      361 ccgcaggcgc ggattggcgg gcagaaccgc cacaggcagc aaatcttacc agagcgtcaa
      421 ggtctcggac tctctttagc aatcgcggcg gcactaccac cccatgacca tgttcgattc
      481 gataactttg cgggcgtgct ccaggtcggt gcccgtttcg tgcatgtaca ggcgaatggc
      541 gtggactttg tcacccgagg ctttcgagag gcaatcgcgg gccgtgccac atacatccat
      601 cttagtgttc gtaattccga gggccccctt gctgatcacc caggtatccc ggggacgctt
      661 cgtcatgcgg atgactttgt cgcttggata ggcctcgcgg acgacgcccc ccgcggcggt
      721 ctcgtcagcg gcccaggtga tgatgatgtg cgcgtcggtt tcaacctcaa acagcggcat
      781 gggaaactcc ttccattgca gaacgggcca gacaagccgg gccggcggct gcgtcggccc
      841 acactccaga gattcacaga taaaagacga ggggaacggg gatcctaggg ctggcatccc
      901 ggggcattcc accgccaatt ctaccacgcc gtcaccggcc gctggagaat cgcggctgcc
      961 ggcccttcac gcccgtaagc atcgcccaac cgctaagatt tgacaagagg agcctttcat
     1021 ctcgtggcca tcgatactcc cgcgaaaatt gcagttctcg gcgccggccc gatcggcctg
     1081 gaggcggcac tgtatgcgcg attcctcggc tatgaagtgg tcgtctttga ggcgggcgaa
     1141 gtggccgcca gcgttcgccg gtgggggcac gtgcggatgt ttacgccctt cggcatgaac
     1201 cgctcgccgc tggggctggc ggcgatccaa gctcaggacg agggctatcg gccgcccggc
     1261 gacgacgaat tgttgacggg ccacgagtgg gtcgatcgtt acctgctgcc gctgtcgcag
     1321 acggacctgc tagccgatca cctgcggctg cagacaaacg tcctccgtgt tggcaaagag
     1381 cgattgctca agggcgaatc gccgggacac gctgaacgcg gcgatttcgc gtttcgattg
     1441 ctggtgcgcg attccgacgg aaacgagcgg attgaaacag ccgatgcggt gatcgatgcc
     1501 agcggcactt ttggactggc gaaccacttg gggcacgggg gcattccagc gcctggcgaa
     1561 gcagcctgcg cggacaaaat cgaatggcgg cttcccgaca ttctgggccg cgaccgcgag
     1621 cactacgcgg gacatcacac gctgctgatc ggcgccggct actcagttgc aaccaatgct
     1681 gtcgccctgt cgcgcctgat cgccgaagtc cccggcacgc gcgtcacgtg gatcacgcgc
     1741 cgcgagccgg cagccggcgc ggctggtcca atcgaggaaa tccacgacga ccggctgcca
     1801 gagcgtgcgg ggttggcgcg tcgggccaat gagttggcga acgacccgaa ttccggcatc
     1861 aattactggc cggctaccga agtcgagcga atcgagggcg aattcagcgt ggaattgtcg
     1921 ggacgccacg ccggcattta ctcgctcgac cgcattatcg ccaacgtcgg ctttcggccg
     1981 aacacacaga tttatgaaga gctgcaagtc cacgagtgtt atgccacgca aggcccgatg
     2041 aagctggcgg cagcgatgat gggccagcaa tcggctgatt gcctcgatca aaaggcctgc
     2101 gggccgcaat cactcctcaa cccggagccg aatttctaca tcctaggcgc gaagagctac
     2161 ggccggaaat ccaactttct gatgtcggtg ggcctggagc agattcgcga gatcttcacg
     2221 atcctcggcg accggccgac gctcgactta tatgctggcg ctcacaacct gctccgatga
     2281 acgatgcctc aaacgaccga cctgccgctc gtcagcattt cccacctgga tcatctctgg
     2341 ttccaggtcg gcggcaccct ctgcaacttg acctgccgac actgcttcat tagctgcagc
     2401 cccaagaatg acagcctcgg ctttctgtcg ctggagcaag tcaagtcacg cctcgaagaa
     2461 tcggtcccac tcggcgtgaa agagtactac ttcaccggcg gcgagccgtt cctgaatccg
     2521 gaaatgactg aaatccttgt tgagacgctc cgctacggac cggcgaccgt cttgaccaac
     2581 ggcacggtgc tcaaagacga atggctgacg cgcctgcgaa cggcggaaga cgctgggccg
     2641 tactcgctgg aatttcgcgt ctcgatcgac ggcttctcgc cagagacaaa cgacccgatt
     2701 cgcggcgaag gaacttttgc gcgggccctg aagggtatcc agaagctcgt gcagcatggc
     2761 tttttgccaa ttgtcacggc ggcccaggtc tggcccgacg agcagaatca cgacttcgtc
     2821 gagcgattcg tcaaagtgct gaaagccgcc ggctactcgc ggccgcggct gaaaatccta
     2881 cccatgctgc gtcttggcgc cgaggaatgt cgcacgcacg actacctggc aagcgagcgg
     2941 gtcgatcgag cgatgctgga gggctacgac acgtcgcagc tcgtctgcga gcacagccgg
     3001 tgcgtcacgg atcgcggcgt gcatgtctgc ccgattctga tcgaatcgcc cgattcgctg
     3061 ctcggccagt cgctcaatgt atcgctgcag ccgttcgcga ttcgccacgg cgcgtgttac
     3121 acgtgctacc agcacggagc gatctgctcg aaccccagct cgctgccggc gcggcaggag
     3181 gccccggcat gagcccgacg cgagtcgccc tgtttggcgg tctttacagt aactacctgg
     3241 cgctgga
//
LOCUS       DASUGB010000034         5771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_144041, whole genome shotgun sequence.
ACCESSION   DASUGB010000034 DASUGB010000000
VERSION     DASUGB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144041"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFV87_00675"
     CDS             complement(<1..219)
                     /locus_tag="VFV87_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102292.1"
                     /translation="MATVPSSTHGTRATPLGVSRYDQVAGLLVAVLVLLGFITLMMFL
                     IWLSTRMFWVAPAVPVTLLDVGGGGSGQL"
     gene            complement(247..1905)
                     /locus_tag="VFV87_00680"
     CDS             complement(247..1905)
                     /locus_tag="VFV87_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5102293.1"
                     /translation="MTHHREQHQILIPAIGWVVGCIWFFTAIEASHAYTPDSPEIKHI
                     IERGMEYLQGSFASEYRLGGKCLIGLCFWKTGRPIDHPAIQAAIKKCRDDEGRKHDQM
                     YDPSIAAIFLCEMEAAQPELHDLAEVYLKKVLSKQKQHGGFGYDGMQTGDTSQTQYAS
                     LALWMAAHHGHQVPNEAVERLAGWLVRTQDPTGTWSYQGNDPGVFQRVQQNPSDRRLS
                     LHVGGAGAMYIVADLLGLTSAAQPEAERKLPPALRAVGKEDDDKRAPRKHLVLKLFDA
                     QLVRRALADGDQFFAKNYAINPAHQLHYYLYGLERYQSFRELAAGREEAEPKWYNEGC
                     EMLMSTQQSTGAWPASGDNTVISSTFALLFLARSSKKSFAGIPSLSDGVLLGGMGLPK
                     NTADIVERDGKLVETPLAGSIDELLALIEKPNAPLDQLIDPTQPLALDGDVTKRAGQI
                     TKLRALVSAGDFNTRLLAVKTLGRARQLDSAPILIYALTDPISQTNPDLRIILEADRG
                     LRFLSRKFGGVGLPSDPKPQDIQTAIKAWKDWYKSIRPDAEFLD"
     gene            2438..4624
                     /locus_tag="VFV87_00685"
     CDS             2438..4624
                     /locus_tag="VFV87_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102294.1"
                     /translation="MTVRCVSLVLCGFVCLSANSLIVGQDAPSPSSGGTSPLGVRQQR
                     VERMMEDLDRKFKSLQIALQQAEPERAERLKQTLDKAKEMLVQKRMGDITKLLDQAQL
                     ENASDSQQALLADIRTLLELLLDEKSDKDKIREEFERLSEWKRDIENILQQERGEKRE
                     TDRLTNKEKTMADLQAKLKALEAAIAQQKEVIAATEAARAEGIQGLGKAAEGQAAARQ
                     TAEKVANQIAKEAGDEKPPFAEKPAAGSSGNQGSGEPKPAEGSKGAEGSKGAEGAKSG
                     EGAKAGEGAKGGEGAKGGEGAKGSEGAKGSEGSSGSEGSQGSQGAEGSQGSQGPPGGR
                     PPEPGEKPLRQAIENQKQAEGNLQEGKGKAAEQEEKTALANLERALAELKKEQSRIAS
                     LPPEHFDKLAKKQDDIANQTGQLEQKMSQGGGQGGGQGGGQGGGQGGGQPKPGQQKVQ
                     QAQKSMQQASGGLKKQDPADANRQQGKAIKDLEEALQEIEERLAQLREETQIEKLARL
                     EARFQEMLLIQKQLSAQTQSLNKKQVENNGTLSRADRNAARGIGDEERRMSPVINPET
                     KVLDKQIGLAGRAQQALDIIIDDGTSVVFPDVVEQVRDDLITVGNLLADDVRIDQYTL
                     TLQKEIEITLEELIEALQQAQKQKEGGGGGGGGGGGEPPLLPNSAELKLLRSAQLRIN
                     RRTAAVEATRPQAGQLDETMKGELQTVARRQAEIAEMTVQILERGQ"
     gene            4742..>5771
                     /locus_tag="VFV87_00690"
     CDS             4742..>5771
                     /locus_tag="VFV87_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102295.1"
                     /translation="MTIRMQALAVILVVGWMGIAAAAPADSNKVLDEFVAAVKANDAV
                     AADQKQVVEKLVKELRSVPEDRAVAITESLRLLHPEYKDALAALGEDNIGAAIVGLTK
                     LRESANPYLAADASYFLARAYLLDERFEDAVPLLDDLQNKWTDKTALGGEVLFLRGVV
                     EVALLRHKEATDTLTAFLTDFPDAPERMRVGAFRQLEQLKLFQEGTLSDVQLRMDFSR
                     RKLTLEDTGTDTREQQDKIIEILAKLIKEAEERECNCKGGGSGSGQKPGGKAGEGEGQ
                     AQGQGSQGGNSGGGSKGIDSDTVKRLHRGGPQSPWSQLRDKDRDPVYSAIKEKFPARY
                     QQLIEQYYK"
BASE COUNT         1269 a         1721 c         1841 g          940 t
ORIGIN      
        1 caattgcccg ctgccgccgc cgccgacatc cagcagggtc accggcaccg ccggcgccac
       61 ccagaacatg cgggtagaaa gccagatcag gaacatcatc agcgtgatga atcccagaag
      121 caccagcacc gccacgagga gtcccgcaac ctgatcgtac cgagagactc ccagcggcgt
      181 ggcccgcgtg ccgtgagtcg aactggggac agttgccatg atttcagatt tcaggaggct
      241 gacaaactag tcgagaaatt cggcgtcggg ccggatggat ttgtaccagt ccttccaggc
      301 cttgatcgcg gtctgaatgt cctgcggctt ggggtcgctc ggcaagccga cacccccaaa
      361 tttgcggctg aggaagcgca atccgcggtc ggcttccaga atgattctca ggtcgggatt
      421 ggtctgggaa atcggatcgg tcagggcgta gatgagaata ggtgcactat ccaattgcct
      481 cgctcgaccg agcgttttca cggccaggag gcgcgtattg aaatcgccag cggaaacgag
      541 cgcccgcagc ttcgtgatct gccccgcgcg cttcgtgacg tcgccatcca gggcgagcgg
      601 ctgagtggga tcgatcaact gatcgagcgg agcattgggc ttctcgatca gcgccaggag
      661 ttcgtcaatg gagccggcca gaggcgtctc gaccagcttt ccatcgcgtt cgacgatatc
      721 ggccgtgttc ttcggcagcc ccattccgcc gagcaacacg ccatcgctga gcgaaggaat
      781 cccggcgaag gacttcttcg agctacgcgc cagaaagagc aaggcaaacg tggaagaaat
      841 gacggtatta tcgccgctcg ccggccaagc gcccgtcgat tgctgcgtcg acatcagcat
      901 ttcgcagcct tcgttatacc acttcggctc ggcctcttcc cgacccgcgg cgagttcccg
      961 aaagctctgg tatcgttcga gaccgtagag gtaatagtga agctgatgcg ctggattgat
     1021 tgcgtaattc ttggcgaaga attgatcgcc gtcggccagc gcgcgccgta cgagctgtgc
     1081 atcaaagagc ttgagcacca ggtgctttcg cggagctcgt ttgtcgtcgt cctccttccc
     1141 cacagcccgc agggcggggg gaagtttgcg ctcggcctcc ggctgcgcgg cgctggtaag
     1201 cccgagcaag tcagccacaa tgtacatggc gccggccccg ccgacgtgca gcgagagccg
     1261 tcgatcgctc ggattctgct gaacgcgttg aaagacgccc ggatcgttgc cttgatagga
     1321 ccaggttccg gtcggatcct gggtgcgcac gagccaccca gccagcctct ccacggcctc
     1381 gtttggcacc tggtgcccgt gatgtgccgc catccagagc gcgagtgagg cgtactgggt
     1441 ttgggaggtg tcgccggttt gcattccgtc atagccaaac ccgccatgct gcttttgctt
     1501 actcaggacc ttcttcaaat acacttccgc cagatcgtgc agctcgggct gcgcagcctc
     1561 catttcacac aagaagattg cagcgatcga tgggtcgtac atctgatcgt gcttccgccc
     1621 ttcatcatcc cggcatttct tgatcgccgc ttgtatcgcc ggatggtcga tgggtctgcc
     1681 cgtcttccag aagcaaagtc caatcaggca cttcccgccc aaacggtatt ccgaagcaaa
     1741 gctgccctgc aggtattcca tccccctttc gataatgtgc tttatttctg gactgtcggg
     1801 agtataggcg tgagacgcct cgattgcggt gaagaaccag atgcaaccca caacccaacc
     1861 gatcgcagga atcaggattt ggtgctgttc tcgatgatgg gtcatttgtc aaccagatcc
     1921 tctgaattga agccagatcc tctgaatgag caaagccggg gcacgcgaca tcgttcccct
     1981 cgcgcgatgc tactcctgac ggcagaaggg gtcaagcaat tcatcgctga gcagctcccc
     2041 tttcgtcgtt accttacgcc cagctgccgg acgcggcaat ccgaggaggc gaagcgctga
     2101 acaggcaaga agaagccggt ttcgaccgct gggtcgcagt gccgtgcgag ccgtactacg
     2161 ccgatggcct tccaagcatc ccgccgtggg cgtacgcccg gatgctgccg gttggccagt
     2221 tcgaggggat cgggcaactg acttcagcag taaaccacgc gagttcgcaa cctctggcgc
     2281 tggagggatt tggggcatgt tgacggaatt ttactttacc aatcggccgc agagtcgtca
     2341 tattagcagg tgatgaactt tgcactccag ccgcgggact aagtgcgcac ctgccggtat
     2401 tggcgtaagt ccattttccc cccctcgcga gattcccatg acagtccgct gcgtgtcgtt
     2461 ggttctatgc ggctttgtct gcctgtccgc gaactccttg attgtcgggc aggacgcacc
     2521 gtcgccgtca tcggggggca cctcgccgct gggcgtccgt cagcagcggg tcgagcggat
     2581 gatggaagac ctggaccgca agttcaaaag cctgcagatc gccctgcagc aagccgagcc
     2641 cgagcgagcc gagcgcctga agcagacgct cgacaaggcc aaggaaatgc tcgtccagaa
     2701 gcggatgggc gatattacga agctgctcga ccaggcccag ctcgaaaacg ccagcgacag
     2761 ccagcaggcg ctgttggccg acatccgcac cctgctggaa ttgctgctgg atgagaagag
     2821 cgacaaggac aagatccgcg aggagttcga gcggctgagc gagtggaaac gcgacattga
     2881 gaacatcttg caacaggagc gaggggaaaa gcgcgagacc gatcgcctga ccaataaaga
     2941 aaagacgatg gccgatctgc aggccaagct gaaggctttg gaagcggcca tcgcccagca
     3001 gaaggaagtg attgctgcga ccgaagccgc tcgggctgag gggattcaag gcctaggaaa
     3061 ggcagcggaa ggccaggccg cagcgcgtca aacggcggaa aaggtcgcca accagatcgc
     3121 caaggaagcc ggcgacgaga agccgccgtt tgcagaaaag ccggcagcag gttcctcagg
     3181 caatcaaggc agcggcgaac cgaagcccgc ggaaggttcc aaaggggctg aaggttccaa
     3241 gggggctgaa ggtgcgaaga gtggcgaggg cgccaaggct ggcgaaggcg ccaagggtgg
     3301 cgaaggcgcc aagggtggcg aaggcgccaa gggctccgaa ggcgcgaagg ggagcgaggg
     3361 ctcatccggc tctgaaggct ctcaaggttc gcaaggcgcc gaaggctctc agggctcgca
     3421 aggaccgccg ggcggccgtc cgccagagcc gggcgagaag cccttgcgcc aggcaatcga
     3481 gaatcaaaag caggccgagg gcaatctcca ggaagggaag ggcaaagcgg ccgagcagga
     3541 agagaagaca gctctggcca atttggagcg ggcgctggcc gagctgaaga aagagcagtc
     3601 gcggattgcc tcgctcccgc cggaacattt cgacaagctg gccaagaagc aggacgacat
     3661 cgccaaccag accgggcagc ttgaacagaa aatgtctcag ggcggaggcc aaggtggagg
     3721 tcagggcggt ggacaaggcg gcggtcaagg cggcggccag ccgaagcccg gccagcagaa
     3781 ggtccagcag gcccagaagt cgatgcagca agcttcgggc ggattgaaga agcaggaccc
     3841 cgccgacgcc aaccgccagc agggcaaggc gatcaaggat cttgaggagg ccctgcagga
     3901 aatcgaagag cgcctggccc agcttcgcga ggaaacgcag atcgagaagc tcgcccgcct
     3961 ggaagcccgc ttccaggaaa tgctgctgat tcagaagcag ctttcggcgc agacccagtc
     4021 ccttaacaag aagcaagtcg agaacaacgg cacgctttcg cgggccgacc gcaacgccgc
     4081 ccgcggcatc ggcgacgaag agcggcggat gagcccggtg atcaatcccg agacgaaagt
     4141 gctcgacaag cagatcggcc tcgcgggccg cgcccagcag gccctggaca tcattatcga
     4201 cgacggcacg agcgtcgtct ttccagatgt tgtcgaacag gtccgcgacg atctgatcac
     4261 cgtcggcaac ttgctggccg acgatgtgcg gatcgaccag tacacgctga cgctccagaa
     4321 ggagatcgaa atcacgctcg aagagctgat tgaagccctt caacaagccc agaagcagaa
     4381 agaagggggc ggcggtggtg gcggcggcgg gggcggcgag ccgccgctgt tgcccaattc
     4441 cgccgagctc aagctcctgc gttcggctca actgcggatc aaccgccgca ccgcggctgt
     4501 cgaagcgacg cggccgcagg cgggccagct cgacgagacg atgaaaggcg agctccagac
     4561 cgtcgcccgg cggcaggccg aaattgccga aatgaccgtg cagattctgg aacgcgggca
     4621 gtagaatcgc ttacattaag taggttccga cggcctcggc ggaaccagca gcggcagccc
     4681 acatcgaccg ccgcggaacc acggaccgaa caccgatcaa tccaaccaac aggaattcgt
     4741 tatgacgatt cggatgcagg cacttgccgt gattttggtc gtcggctgga tgggaattgc
     4801 cgcggctgcg ccggcggatt ccaacaaggt cctcgatgaa ttcgtggcag ccgttaaagc
     4861 gaatgatgcc gttgctgccg accagaagca ggtcgtggag aagctcgtga aagaattgcg
     4921 gtccgtcccc gaagaccggg cggtggcgat caccgagtcg ctccgcctgc tgcatccgga
     4981 gtataaggac gcgctggcgg cgctgggcga agacaacatc ggcgcggcga ttgtcggcct
     5041 gacgaagctc cgcgaatcgg ccaatccgta cctggcggcc gatgcgagct acttcctcgc
     5101 gcgggcctac ctcctcgacg agcgattcga agacgccgtg ccgctgctgg atgacctgca
     5161 gaacaaatgg accgataaga cggctctggg cggagaggtg ctgttcctcc gcggcgttgt
     5221 cgaagtcgct ctgctgcggc acaaggaagc gactgacacg ctgaccgcct tcctgaccga
     5281 ttttcccgac gctcctgagc ggatgcgggt cggtgcattc cgccagctcg agcaattgaa
     5341 actcttccag gaagggacac tcagcgacgt gcagctccgc atggatttca gccgccgcaa
     5401 gctgacgctg gaagacacgg ggacagatac ccgcgagcag caggacaaga tcatcgagat
     5461 cctcgccaag ctcatcaagg aagccgaaga gcgggaatgc aactgcaaag gcggcggctc
     5521 cggcagcggt cagaagcccg gcggcaaggc gggcgaaggc gaaggccagg cccagggcca
     5581 gggaagccag ggcggcaact ccggcggcgg cagcaaggga attgacagcg acacggtgaa
     5641 gcgcttgcac cgcggcggcc cgcagagccc ctggagccag ctccgcgaca aggaccgcga
     5701 ccccgtctat agcgccatca aggaaaaatt ccctgcccgc tatcagcagc tcatcgagca
     5761 gtactacaaa a
//
LOCUS       DASUGB010000035         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1811235, whole genome shotgun sequence.
ACCESSION   DASUGB010000035 DASUGB010000000
VERSION     DASUGB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811235"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFV87_00695"
     CDS             <1..678
                     /locus_tag="VFV87_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102296.1"
                     /translation="NPLRQVLMHFLPAELAGQPLPNKDSNDDRYELLLKLQGLLETLG
                     HRGIIVLVDRLDEPHLVNGSAELMKALLWPMLDNKFLKHPGMGLKLMLPIELTRFIER
                     EERDFYQRARLDKQNMVPSFEWTGEALYDVATARLRACAKPGAQPALSDLFDPGVSQQ
                     RMIEAFRTLRVPRRLFKFLYRLIADHCNAHTDQDPDWKISSTLFETTLAVHLREQDAF
                     DRGVGAG"
     gene            complement(687..980)
                     /locus_tag="VFV87_00700"
     CDS             complement(687..980)
                     /locus_tag="VFV87_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102297.1"
                     /translation="MLLPLQFNDGSQVPPEQLAEAVLEVVGHFGAASYETQKVEGHWR
                     HEGVIYRDDLVKIVVDVPDTAANRTWMRQFKTRWKARLQQLELWLVSYQIEVD"
     gene            complement(955..1179)
                     /locus_tag="VFV87_00705"
     CDS             complement(955..1179)
                     /locus_tag="VFV87_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102298.1"
                     /translation="MITITFPDRATEKRGLAYLLGRFSGRVLSTGEHLVPEAALEALA
                     SEGISFTVHSKATYDQQVAALRSAAAAPVQ"
     gene            complement(1257..1934)
                     /locus_tag="VFV87_00710"
     CDS             complement(1257..1934)
                     /locus_tag="VFV87_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102299.1"
                     /translation="MQVQEQVGTRAQAAEEFKLHEDRRDAERVVESLVGGLTLLTETC
                     QSRVFTDVLKVFPQDSMQVRFPAKGELSGKHEIDLLLVAESASFAGDQRLIRSERAWY
                     ASWLAALRLGETAHDPQAQKRLAIYADLPGDGRRLAFQKVLDKAIPESRRAPLVLLRL
                     LPLAAGIVTAAAFGNLDLARSLRKQQTSLLPAILDCHRCHGRLLDCGDTCPECGNPLW
                     KYNWLTS"
     gene            2104..>3066
                     /locus_tag="VFV87_00715"
     CDS             2104..>3066
                     /locus_tag="VFV87_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102300.1"
                     /translation="MPRFLIACIAASACFVPASFAADAWKPASGPLMTRWAKDVSPEK
                     VHPEYPRPQMVRQQWTNLNGLWDYAIVDRPADAEDYSEPQAWDGQILVPFPIESSLSG
                     VRKQVGDGKRLWYRRTFKASKPADGGRLLLHFGAVDWHAVVFVNDAEIGEHRGGYDPF
                     TFDITDALDAGKEEQVLLVAVHDPNDANWQPRGKQVNQPKGIWYTPTTGIWQTVWLEE
                     VPVTYIKSIQITPDIDNEQFEVVVNLAGDDADPDKLAPRFAPFGFEVLDGDRQIQKTY
                     ANDAMSGKQVKIRMLSKPKLWSPESPSLYSLKVQIGLKQHPVSKA"
BASE COUNT          590 a         1012 c          918 g          546 t
ORIGIN      
        1 aatccgctgc ggcaggtgct gatgcacttc ctcccggcgg aactcgccgg ccagccgctg
       61 ccgaacaaag acagcaacga cgaccgctac gagctgctgc tcaagctcca gggccttctg
      121 gaaacgctcg gccaccgcgg gatcatagtc ctggtcgatc gcctcgacga gccccacctg
      181 gtcaacggct cggccgagct gatgaaggcc ctgctctggc caatgctcga caacaagttc
      241 ctcaagcacc cggggatggg cctgaagctg atgctgccga tcgagctgac gcggttcatc
      301 gagcgcgagg agcgcgactt ctaccagcgg gcccggctcg acaagcagaa catggtgcct
      361 tcgttcgagt ggacgggcga ggcgctctac gacgtagcga ccgcgcgctt gcgggcctgt
      421 gccaagccgg gcgctcagcc ggccttatcc gatctgtttg accccggcgt cagccagcag
      481 cggatgatcg aggccttccg caccctgcgc gtcccccggc gactgttcaa attcctctat
      541 cgcctgatcg ccgaccactg caacgcgcac actgatcagg accccgactg gaagatttcc
      601 tccacactct ttgagacgac gctcgcggtg catctccgcg agcaggacgc gttcgatcgg
      661 ggcgtcgggg cgggatgagg ccgccgtcaa tcaacctcga tctggtagct gacgagccac
      721 aattccagtt gctgaagccg cgctttccac cgcgttttaa attgccgcat ccacgtgcga
      781 tttgccgccg tgtcgggcac atccaccacg atctttacga ggtcgtcccg gtagatcacg
      841 ccttcatgcc gccaatgacc ttcgactttc tgggtctcgt agctggccgc tccaaagtgg
      901 ccgaccactt cgaggacggc ttccgcaagc tgctccggcg gcacctggct gccgtcattg
      961 aactggagcg gcagcagcac ttcgaagcgc cgccacttgc tggtcgtacg tcgctttgct
     1021 atggacggtg aacgagatac cttcgctggc gagagcttcc agcgccgctt ccggaacgag
     1081 gtgctcgccc gtgctcagca cgcggcccga gaaccgcccc agtaaatagg ccaagccgcg
     1141 cttttcggtc gcccggtcgg gaaatgtgat cgtaatcatc ggcaagcctc cgcatcaatc
     1201 ataggctgcc ggcagcggcg tggccaattg cgatcggcga accgccaaga ttcgcttcag
     1261 ctcgtcagcc agttgtactt ccacagcggg ttgccgcatt cggggcaggt gtcgccgcag
     1321 tcgagcagcc ggccgtggca gcggtggcag tcgaggatgg cgggcaacag cgacgtttgc
     1381 tgcttacgca gcgagcgggc cagatcgaga ttgccgaaag ccgccgcggt cacaattcct
     1441 gccgccagcg gcaaaaggcg caacagcacc agcggcgccc gccgcgattc gggaattgcc
     1501 ttgtcgagca ccttctgaaa ggccagccgc cggccgtcgc cgggcagatc ggcatagatg
     1561 gccagccgct tctgagcctg cggatcgtgg gccgtttcgc ccagccgcag cgcggccagc
     1621 cagctcgcat accaggcccg ctcgctgcga atcagtcgct ggtcgcccgc gaagctcgcc
     1681 gattcggcaa cgaggagcag gtcgatctca tgcttgccag aaagttcccc cttggcggga
     1741 aagcgaacct gcatcgaatc ctgcggaaag accttgagca catcggtgaa gacccgcgat
     1801 tggcaggtct cggtgagcag cgtcaggccg cccaccaggc tttcgaccac ccgctcggca
     1861 tcccgccgat cctcgtgcag cttgaactcc tccgccgcct gcgcccgcgt gccgacttgc
     1921 tcttggactt gcatggccgc cctccgccag atgccttcga gtgctgaacc agacgccccg
     1981 cattatcgca ctcctgcggg gcacaagcta caagagggcc cgccacttcc acgacgatca
     2041 gccgcggagt accgtaggaa tcgctcgctt gtctggcatc ccttctcccg gcaggaaatc
     2101 gccatgccac gcttcttgat cgcctgcatc gctgcttctg cgtgttttgt tccagcgtca
     2161 ttcgcggcag atgcctggaa gcccgcctcc ggcccgctga tgacgcggtg ggcgaaagac
     2221 gtttcgcccg agaaggtcca tcccgaatat ccgcggccgc agatggtccg ccagcagtgg
     2281 acgaacctga acggactgtg ggactatgcg atcgtcgatc gtccggctga cgcggaggat
     2341 tattccgagc cgcaagcttg ggacggccag atcctggtgc cgttcccgat cgaatcctcg
     2401 ctctccggcg tgcggaagca ggtcggtgac ggcaagcgcc tgtggtaccg ccggacgttc
     2461 aaggcctcga aacccgcgga cggcggacgg ttgctgctgc acttcggcgc ggtcgattgg
     2521 cacgcggtcg tgttcgtcaa cgacgcggaa atcggcgagc accgcggggg ctacgatccg
     2581 ttcacgttcg acatcaccga cgcgctcgat gcgggcaaag aagaacaagt cctgctcgtc
     2641 gccgtccatg accccaacga cgccaactgg cagccccgcg gcaagcaggt caatcagccc
     2701 aaaggcatct ggtacacccc caccaccggg atctggcaaa cggtatggct ggaggaggtg
     2761 ccggtgacct acatcaagtc gatccaaatc acaccagaca ttgataacga acagtttgaa
     2821 gtcgtcgtga atctcgcagg agacgatgcc gacccggata aattggcacc gagattcgca
     2881 ccgttcggct tcgaagtgtt ggacggagat cggcaaatcc aaaagacgta tgcgaacgac
     2941 gcaatgtccg gaaagcaagt caagatccga atgttgagca aaccgaagct ctggtcgcca
     3001 gaatcgccgt cgctttactc cttgaaggtt caaattggcc tcaagcaaca cccggtttcg
     3061 aaggca
//
LOCUS       DASUGB010000036         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1811253, whole genome shotgun sequence.
ACCESSION   DASUGB010000036 DASUGB010000000
VERSION     DASUGB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811253"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFV87_00720"
     CDS             <1..278
                     /locus_tag="VFV87_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5102301.1"
                     /translation="ATYDLLGWALFHQQKYSEAEAFFRESIRLSPDKVLVHCGLGCSL
                     VEQRKFAEAETTLREALRLDPQHRWATGLLKRALDGQGKPPENVEPK"
     gene            542..1690
                     /locus_tag="VFV87_00725"
     CDS             542..1690
                     /locus_tag="VFV87_00725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX5102302.1"
                     /translation="MRFSAARPLRLESLEERRLLTFLPAASDPAGLIALAANLAGGES
                     AQSHAAGLGGRPAAPAVRLDVVALHEFGHSLGLSHSEDPNSIMYPYYNAGYDTSNLAD
                     DSAVEALLEIYADVHSSGWNDLHDADGGISDGDVDLTYSFVPDGVRMDRGGFSTLYAT
                     FDGLFGPDQWQQIFAGELARWASVSDGKLSFHEVPDAGLPFNYRGEAQNDPNAGDIRI
                     GAHRFDSIGKVLALTWFPPPGGYTLAGDANFDQDENWVLEGGAEASRVGPAGGSASVA
                     SNGLTWAGDGEALAVLAWIDPSSQTRAVDSTSDVLRTAAEEISPAGQEPMGRFAATAQ
                     SGLDSQATDLLLKAGSHTGHACPDDSAFADPDLLDPVWLEPLSRPQDQ"
     gene            2124..>2542
                     /locus_tag="VFV87_00730"
     CDS             2124..>2542
                     /locus_tag="VFV87_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5102303.1"
                     /translation="MSVLLGNGSGEFAAPLNFAVGEDPTSVAVGDFNGDSRPDLAVTN
                     LGSDNVSVLIGDGTGNFGIAPSVTVGERPSSVAVGDFNGDGNTDLAVANGKSYTVSVR
                     LSDGAGNFEVAPTIATGDFPRDIAVGDFNGDGRLDLAV"
BASE COUNT          484 a          800 c          783 g          475 t
ORIGIN      
        1 aagcaacata tgacttgctc ggctgggctt tgttccatca gcagaaatac tccgaggccg
       61 aggcattctt tcgcgaatcg attcgtctga gccctgataa ggttttagtc cattgtggac
      121 tcggttgttc tctcgtcgag caaagaaaat ttgcggaggc ggaaacaaca ttgcgcgagg
      181 cattgcgcct cgatccacag caccgctggg ccaccggtct gctcaagcgg gcgttggacg
      241 gccagggaaa accgccggaa aacgtggaac caaagtgagg caagaaatgc tcaagccgaa
      301 gtagctaagt ccgctcccga acaacaaaag cggccggtga ggccctaacc gtcaagaaag
      361 ctcctcaccg gcccggcacc gccccggcgc tcggtctgcg cgctgggtgg gtcgccgatg
      421 ccattttatt gcggaccgca tccccctgaa aggttctcct atgtcagtcc gcacgctgct
      481 tcaagtgttg aaatccaggc tcttggtcgg gccagcttgc cgttggcgac aagcaccttc
      541 agtgcgcttc tctgctgctc gcccgctgcg actggaatcg ctcgaagagc gtcgtttgct
      601 cacctttctg cctgctgcga gcgaccccgc cggccttatc gcgcttgccg ccaacctcgc
      661 gggtggcgag tccgcgcaat ctcacgctgc agggctgggt ggcaggcctg cagcgccggc
      721 ggtccgactc gatgtggtcg ccctacacga attcggacac tcgctcgggc tgtcgcacag
      781 cgaggatccg aattcgatca tgtatccgta ctacaacgcc ggatacgaca cgagcaacct
      841 ggccgatgac tccgcggtgg aggcgttgct ggagatctat gccgatgtcc actcgagtgg
      901 ttggaacgac ctgcacgacg cggacggcgg catcagcgac ggcgacgtcg atctgaccta
      961 cagcttcgtg cccgatggag tgcgaatgga ccggggaggg ttcagcacgc tctatgcgac
     1021 tttcgacggt ctatttggcc ccgatcagtg gcagcagatt ttcgctggag aactggcgcg
     1081 ctgggcctcc gtcagcgacg gcaaactcag cttccatgaa gtgcccgacg cagggctccc
     1141 gttcaattac cggggggaag cgcagaacga ccccaatgct ggagacatcc ggattggcgc
     1201 tcaccgcttc gactcgatcg gtaaagtgtt ggcgcttacc tggttcccgc ctcccggtgg
     1261 ctacacgctc gctggcgacg ccaacttcga tcaggacgag aactgggttt tagaaggtgg
     1321 cgccgaggcc tcccgcgtcg ggcccgctgg agggtcggcc tcagttgcca gcaatggttt
     1381 gacgtgggcc ggtgatggtg aagcccttgc tgtgctcgcc tggattgacc cttccagcca
     1441 gacccgtgct gtggactcga ccagtgatgt tctccgaacc gctgccgagg agatcagccc
     1501 ggccgggcaa gagccgatgg gtcggttcgc cgcgacagcg cagtctggct tggacagtca
     1561 agccacagat ttgctgttaa aagcaggcag ccacaccggg cacgcctgcc cagacgactc
     1621 tgcttttgcg gacccggacc tactcgaccc cgtatggcta gagccgctgt ctcggccgca
     1681 agaccagtag gcggaagccg gagagagttc atcccgacgg atgaatcccg ccaaatctcg
     1741 aaattcaaag cggccggtga ggccttaccg ccaagcaagt tcctcaccgg cccggcacgg
     1801 ccccggcgct cggtctgcgc gctgggcgcg atgtcttttc atcatatcgc aggccgattc
     1861 ctgaaaggaa aagtacgatg cgattcttta cctggctgcg accactgaca agtagcggcg
     1921 cccgcgcggc gcaacgccga cccgcgccaa ctaattggcg gccaagattc gaaccgctcg
     1981 aggatcgacg atgcctgagc ggcgtctcgt tcgggcaagc catcaactgg gcggctggcg
     2041 acgggccaaa ttccgtcgca gtgggggact tcaacggcga cggccgccca gacctagctg
     2101 cgaccgattt cgactcgaac agcgtgagcg tactgctcgg caatggcagc ggcgagtttg
     2161 ccgccccgct caacttcgcc gtcggcgagg atcccacttc agtggcggtg ggggacttca
     2221 acggcgacag ccgccccgac ctagccgtga ccaatctagg ctcggacaat gtaagcgtgt
     2281 tgatcggcga tggcaccggc aacttcggga ttgcaccctc cgtcaccgtc ggcgaaaggc
     2341 cgtcttctgt tgcagtgggc gacttcaacg gcgatggcaa caccgacctg gccgtggcca
     2401 acggcaaatc ctacaccgtg agcgtgcggc tcagcgatgg cgctggcaac ttcgaggtgg
     2461 cgccaaccat cgctaccggc gacttccctc gtgatattgc ggttggagac ttcaacggcg
     2521 acggccgcct cgacctggcc gt
//
LOCUS       DASUGB010000037         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1116502, whole genome shotgun sequence.
ACCESSION   DASUGB010000037 DASUGB010000000
VERSION     DASUGB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116502"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFV87_00735"
     CDS             complement(<1..1073)
                     /locus_tag="VFV87_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX5102304.1"
                     /translation="MSVSTLPKTLTVALLGNPNTGKSTLFNAISGLRQRVGNYPGVTV
                     EKKLGRTEFHGRSFTIIDLPGTYSLAPRSPDEMVAVDVLLGRRRDVPPIDVVLCIVDA
                     SNLERNFYLLSQVLEFSLPTVVALNMVDVAKDKGLEFDLQKLQQQLPVKFVPVQANKG
                     VGIAALKQALIDVADSSRPPAASPFPEVFVREVDQLHRRVNSNGRPALPRYLAERLLL
                     DTTGYLAESGLLGMNRELLSDVNESRARLAKAGLAVPGVEAMARYQWAADVLSGVVHR
                     PQERKITLSDRIDRVLTHRIWGTLVFLAVMLLMFQAVFYVADPASAAVDWLNARLAAL
                     VTANVPDGMLQSLLVTGVIGGVGG"
     gene            complement(1086..1316)
                     /locus_tag="VFV87_00740"
     CDS             complement(1086..1316)
                     /locus_tag="VFV87_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX5102305.1"
                     /translation="MTTLAALAVGARGRIVRITDDDEISIRLMEMGLTPSTDLAVVGR
                     APLGDPLELEVRGYRLSIRQSEAQRVEVEPLP"
     gene            complement(1313..1549)
                     /locus_tag="VFV87_00745"
     CDS             complement(1313..1549)
                     /locus_tag="VFV87_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX5102306.1"
                     /translation="MHDLLPIQLLPAGSRARIDQLLGGEDDVHRLQELGMRVGTQIEI
                     VQSGVPCIIKVDGTRFCFRDSESLGVLVRLGEVA"
     gene            complement(1643..>3142)
                     /locus_tag="VFV87_00750"
     CDS             complement(1643..>3142)
                     /locus_tag="VFV87_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5102307.1"
                     /translation="LLNAVVVRRFGEALKEAAAADAQLAAGFTPTRSVSEGAPLVGVP
                     ITIKECFHLAGTPCTIGIDSSERQEIFQHEAVLVRRLKQAGAVILGKTNVPQLMIWHE
                     CDNPVYGRTNNPWDLDRTCGGSTGGEAAIVAAGGSPLGLGNDLGGSIRVPCHFCGIHG
                     LKPTSYRLPRGGVTVALRGFESLVTQPGPMARHVEDLWLAMQVLAEDSDGYVAGDVVP
                     AKLPDPHEVRIDQLTIAVLPDDGVFPASPAIQRAVRESADALRERGAKIVELAPADFG
                     GSLSLEEVFDMYCGFIGADGAADCRRFLQGSTVDWRVWRMVTLASLWSLSRWALALGL
                     KLGGQRWMARLLEVARPLSADEFWQLTHRKTAYVASVISGLKARGIDAILCPPHALPA
                     MQHTKGFDLLAAASYAYLFNLLSFPAGVVSLTRVRSDEEDSRPDSRDRVLQEAREVDL
                     GSAGLPVGVQVAGLPWREDIVLAVMSALERSFCEKPDYPCNIAVPTSKN"
BASE COUNT          617 a          985 c          976 g          564 t
ORIGIN      
        1 ccgcccacgc caccgatcac gcccgtaacg agcaacgact gaagcatgcc atcgggcaca
       61 ttcgccgtta cgagtgcggc gagtcttgca ttgagccaat ccacagcggc agaggcgggg
      121 tcagcgacgt aaaacaccgc ctgaaacatc agcaacatta cagcgaggaa tactagcgtt
      181 ccccagatgc ggtgcgtgag gactctgtca attctgtcgc tcagcgtgat ctttcgctcc
      241 tgcggccggt ggacgacacc ggagagtacg tccgccgccc actgataacg ggccatcgcc
      301 tcgacgcccg gtacggccag tccggccttc gcgagccgcg cgcgggattc gttcacatcc
      361 gacaagagct ctcgattcat ccccagcagg cccgactcgg ccaggtagcc ggtggtatcg
      421 agcaacaggc gctcagccag gtagcgcggc agcgcagggc ggccattcga attgacgcgc
      481 cggtgcagtt ggtccacttc gcggacaaaa acctccggga atgggctggc ggcggggggc
      541 cgcgacgaat cggccacgtc gatgagcgcc tgcttgagcg cggcgatgcc cacacccttg
      601 ttggcctgga cggggacgaa cttcaccggc aattgctgct ggagcttctg caggtcgaac
      661 tccaaaccct tgtcttttgc gacatcgacc atattcaggg cgacgacggt gggcaggctg
      721 aattccaata cctggctgag caggtagaaa ttccgctcca ggttgctcgc atcgacgatg
      781 cacagtacga cgtcgatcgg cggcacgtcg cgcctgcggc ccaaaagcac gtcgacggcg
      841 accatctcgt cgggcgagcg cggcgcgagg ctgtaagtgc cggggagatc gatgatcgtg
      901 aagctgcggc cgtggaattc tgtgcggcca agcttcttct ccaccgtgac gcccgggtaa
      961 ttgcccaccc tctgccgcaa gccggagatg gcgttaaaca gcgtcgattt gcccgtattt
     1021 ggattgccga gcagcgcgac ggtgagcgtt ttgggcaggg tggaaacgga cacggcgatc
     1081 gcgagttacg ggaggggttc aacttccacg cgctgggctt cgctttggcg gatgctcaag
     1141 cgatagccac ggacctccag ttccagcgga tcgccgagcg gagccctgcc aacgacggcc
     1201 aagtcggtcg agggggtgag ccccatttcc atcaagcgga tgctgatctc gtcatcgtcc
     1261 gtaattcgaa cgatgcggcc gcgggcgcca accgccaggg cggccagggt tgtcatgcga
     1321 cttctcccaa tcgcaccagc actcccaacg attcgctatc gcgaaaacaa aatcgcgtac
     1381 catccacttt gatgatgcac ggcacaccgg actgcacaat ttcaatctgc gtaccgacgc
     1441 gcatccctaa ttcctgcagg cggtgaacat cgtcctcgcc tcccaggagt tgatcgatac
     1501 gagcccggct gccggcgggc aggagttgga tcggtagcag atcgtgcatg tcgcgatgca
     1561 atatcgccaa tcggcgacgc ctgttgaaac tgagtctcac taaaatgata caggctttac
     1621 acggcgaatc aacggcgcga aatcagtttt ttgatgttgg aaccgcaata ttgcagggat
     1681 agtctggctt ttcgcagaaa ctcctttcca gggctgacat taccgcgaga acgatatctt
     1741 cccgccacgg caagcccgct acctggacgc caacgggcag gccggcgctc cccagatcta
     1801 cttcccgtgc ctcttggagg acgcgatcgc gagaatcggg gcgggaatcc tcttcgtctg
     1861 atctcacgcg ggtgagcgac acgactcctg cgggaaaact gagcagattg aaaagatacg
     1921 catagcttgc tgccgctagc aggtcgaagc ctttggtgtg ttgcattgcg ggcagggcgt
     1981 gcggcgggca gaggatcgca tcgatcccgc gcgctttcag gccgctgatt acggacgcga
     2041 cgtacgcggt ctttcgatgg gtgagttgcc agaattcgtc agccgaaagc ggtcgggcca
     2101 cttccagcag ccgggccatc caccgctgtc cgcccagctt caacccgagg gccagcgccc
     2161 atcgactaag cgaccagagg ctggccagcg tcaccatccg ccagacgcgc caatcgaccg
     2221 tgctgccctg gagaaagcgg cggcagtcgg cggcgccgtc ggcgccgatg aacccacagt
     2281 acatatcgaa gacttcttcg agcgagagcg aaccgccaaa atcagccggc gccagttcga
     2341 caatcttggc gccgcgctcg cggagtgcgt ctgccgattc gcgcacagcc cgctgaattg
     2401 ccggggaagc tggaaagacg ccatcgtccg gcaacacggc gatggtcagt tggtcgatgc
     2461 gcacttcgtg tgggtcgggc agctttgccg gcacgacatc acccgccaca tatccatcgg
     2521 aatcttcggc cagcacctgc atcgccagcc acagatcctc gacgtgccgt gccatcggtc
     2581 ccggctgtgt gacgagtgac tcaaagccgc ggagggcaac ggtcacgccg ccgcgcggca
     2641 agcggtagct cgtcggcttc aggccgtgga ttccgcagaa gtggcagggc acgcgaatgc
     2701 tgccgcccaa gtcattgccc aggcccagcg gcgagccgcc agccgcgacg atcgcggcct
     2761 caccgccggt gctgccgccg caggtgcggt ccaggtccca cggattgttc gtgcggccat
     2821 aaacggggtt gtcacattcg tgccagatca tgagttgcgg cacattcgtc ttgccgagga
     2881 tcaccgctcc agcctgcttt agccgccgga cgaggactgc ttcgtgctga aagatctcct
     2941 gacgctccga tgagtcgatg ccgatggtgc agggtgtgcc ggcgaggtga aagcattctt
     3001 tgatcgtgat gggcacgccg acgagcggcg cgccctcgct gacgcttcgg gttggtgtga
     3061 agccggcagc gagctgcgca tcggcagcag cggcttcctt caatgcttcg ccaaatcgcc
     3121 tgacaacgac cgcgttgaga ag
//
LOCUS       DASUGB010000038         5312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_699722, whole genome shotgun sequence.
ACCESSION   DASUGB010000038 DASUGB010000000
VERSION     DASUGB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699722"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VFV87_00755"
     CDS             complement(<1..1497)
                     /locus_tag="VFV87_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5102308.1"
                     /translation="MPFLLRVLCALLFLLPAAAPLRAAEEPVYDLVIYGGTSGGVAAG
                     VQARRMGKTAVVIEPGKHLGGLTSGGLGATDIGNKGAIGGISREFYQRIMKHYADPAS
                     WTQQKPEEYRPSRGSDTTKEDAMWTFEPHVAERIMEDLCKENGIVVVKSERLDLEKGV
                     TKDGSRIVSIRMESGQTFHGKMFIDATYEGDLMAKAGVSYTLGREANSQYDETLSGVQ
                     VANATKHQFVKPVDPYAKPGDPQSGLLPRVVAGPMPPDGTGDKKVQAYNYRLCATDKP
                     ENRRSWPKPANYDEKQYEILLRNFEAGDLRLPWNPVLMPNHKTDSNNNFAFSTDNIGM
                     NYDYPDGDYATRKKIVQEHIDYQQGLMWTLANHPRVPQSVRDHFTKWGLAKDEFLDTD
                     NWPHQLYVREARRMVGGYVMTQHNCQGRVTAEDSVGLAAYTVDSHNIQRYVDANGHVR
                     NEGDVQVGGFPPYPISYRSLVPKEAECTNLFVPVCLSATHISYGSIRME"
     gene            complement(1540..2217)
                     /locus_tag="VFV87_00760"
     CDS             complement(1540..2217)
                     /locus_tag="VFV87_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102309.1"
                     /translation="MDTPSADEESYRLLNDNLRTDYGECPFVVDAAFQSSVTVEHFSG
                     GDDATYVFVSRGLAWSDSSRAAKPTIRFAPFVAGTDIIYDVTEATLQGKARPFECDLR
                     QHSRKIYALMPFQVEVIQVSYRIVRDDRHALISFHDARGERIRAVSPFEFQTDFDPGK
                     TCVINYGVPKRLRATGRDGVYTEVLRGPQMTPGTQTFLVRSLLTGNQQKAAFSAWSTV
                     VPVVPSE"
     gene            complement(2241..3407)
                     /locus_tag="VFV87_00765"
     CDS             complement(2241..3407)
                     /locus_tag="VFV87_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5102310.1"
                     /translation="MPRPEDVCPRPQPLPPLATTPHAPPIWPASVWECESPQQADAML
                     GGQQPGYVYQRDGHPNAWLLAEKCRQLHGADRAAIAASGMGALAAALLSQLKAGDHCV
                     VGGRVYGKTHVLFGAEASRLGIEMTTVDTCDLKATAAAFKPNTKLLLAETIANPLLEV
                     ADIAALAEIAHSRKAALLIDNTFASPILCRPLELGADLVMESLTKTMNGHSDVVLGLL
                     CGREAVFERVPAVISAWGLSSSPFDCWLADRGLATAHLRIERACDNAFAAAEFLHSQK
                     KQIERVFYPGLSSHAQHALAKRQFMGRYGTIVSFNFAGGRPAADAFIAAAKQIPFCPS
                     LGELSTTLSHPETTSHRGLSADERAALGITGGTIRLSVGTESNEFIQSALQQGL"
     gene            3586..4626
                     /locus_tag="VFV87_00770"
     CDS             3586..4626
                     /locus_tag="VFV87_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102311.1"
                     /translation="MPARDPQVVAFAPGGTIIATGCSGMTDSTFPPRPHPDVRKCGVV
                     AVWDVATRKRLFRWETYGDLTKLAFSPDGTLLAASRLFETPDGLALHEVRVWDVTSGR
                     VVKSLDRCHAFDFAPIEPQIAVLSRSKCVVYDLQDWTKEKLVAPLGGAVSIAFTADGL
                     SLIGVVKDEGKYRLRLTSVESNAAGETPTPRGAGILPATQSQALDEPFYRAAVVADGS
                     LLATGHGGGNVVLWDAATLDVRGRLQTGVRGLAHPFFSPDATLLAAGCQENGDVVIWN
                     LQQRQEAGRFTFEKGGLRTYMQRPANATFRPEEDPTRFCFSPGGEAILVGAYGGILRA
                     TSGGQELGRFGD"
     gene            4853..5092
                     /locus_tag="VFV87_00775"
     CDS             4853..5092
                     /locus_tag="VFV87_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5102312.1"
                     /translation="MSLEEFLEWRKGAGDAPLTRQLQAAIAATGQSLYAVAQASGVAA
                     PVLQRFINGERGITLETAGKLAAYLGLSLMPESRP"
     gene            5089..>5312
                     /locus_tag="VFV87_00780"
     CDS             5089..>5312
                     /locus_tag="VFV87_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102313.1"
                     /translation="MTLQSLLGEQPVSRFIAEHYQRLPYSLAGGADSLRELGTWESLT
                     AILGQPAADLLVCRRNERYDGPAPRTADDAR"
BASE COUNT          919 a         1668 c         1704 g         1021 t
ORIGIN      
        1 ctccatgcgg atgctgccat agctgatgtg cgtggcggaa aggcaaaccg gtacaaacag
       61 gttcgtgcat tcggcctcct tcggcacgag cgagcggtac gaaatgggat agggcgggaa
      121 gccgccgact tgcacgtccc cttcgttgcg gacgtggcca ttggcatcga cataccgctg
      181 gatgttgtgc gaatccaccg tgtaagcggc caggccaacg gaatcttccg cggtcacgcg
      241 gccctggcaa ttgtgctgcg tcatcacgta gccgccgacc atcctccggg cctcgcggac
      301 gtaaagctgg tgcggccagt tgtcggtatc gagaaactcg tccttcgcca gcccccattt
      361 cgtaaagtgg tcgcgcaccg actgcggcac gcggggatga ttggccagcg tccacatcag
      421 cccttgctgg tagtcgatgt gctcctgaac gatcttcttg cgcgtcgcgt agtcgccgtc
      481 gggatagtca tagttcatcc cgatgttgtc ggtcgagaag gcgaaattgt tgttcgagtc
      541 ggtcttgtgg ttgggcatca acaccgggtt ccacggcagg cgcaggtcgc cggcttcgaa
      601 attccgcagc aggatttcgt actgcttttc gtcatagttc gccggcttgg gccacgatcg
      661 gcggttttcg ggcttgtccg tcgcacaaag gcggtagtta taggcctgga ccttcttgtc
      721 gcccgtgcca tcgggcggca tcgggccggc gacgacccgc ggcaacaggc cgctctgcgg
      781 atcgcccggt ttcgcatagg gatcgacggg cttgacgaac tggtgcttgg tggcgttggc
      841 gacctgcacg ccgctcagcg tttcgtcgta ctggctgttg gcttcgcggc cgagcgtgta
      901 gctgacgccc gccttggcca tcaggtcccc ttcgtacgtg gcatcgatga acattttgcc
      961 gtgaaaagtc tggcccgatt ccatccggat gctcacgatc cggctgccat ccttggtgac
     1021 gcccttttcc aggtccagcc gctcgctctt gacgacgacg atgccgtttt ctttgcagag
     1081 atcctccatg attctctccg ccacgtgcgg ctcgaatgtc cacatcgcgt cttccttggt
     1141 cgtgtcgctg ccgcggctgg ggcggtattc ctcgggcttc tgctgcgtcc agcttgccgg
     1201 gtcggcatag tgcttcatga tccgctgata aaactcgcgg ctgatgccgc cgatcgcccc
     1261 tttgttgccg atatcggtcg cgccgagccc accgctcgtc aatccgccca gatgcttccc
     1321 cggctcgata accacggccg tcttccccat ccgccgcgcc tgcacccccg cggcaactcc
     1381 gccgctggtg ccgccgtaga tcaccaggtc gtaaaccggc tcctccgccg cgcgcagtgg
     1441 agcggcagcc ggcaggagaa acagaagggc gcaaaggacg cgaagaagaa atggcatgac
     1501 tgatcgggaa atcaagagtg gagatggtac tgcagcagct tactccgacg gtactaccgg
     1561 gacgaccgtg ctccacgcag agaacgccgc cttctgctgg tttccggtca gtaaagatcg
     1621 gaccagaaag gtttgcgtcc caggcgtcat ttgtggtccg cgaaggacct ctgtatacac
     1681 accgtctcgg cccgtggccc gcaagcgttt gggaacaccg tagttgatca cgcacgtttt
     1741 gccggggtca aagtcggtct ggaattcaaa gggggaaacc gctcgtattc gttcaccgcg
     1801 ggcatcgtgg aaactaatca gggcgtggcg gtcgtcacgt acgatgcggt aacttacctg
     1861 aatgacctca acctgaaacg gcatcaacgc gtaaatcttc cgcgaatgtt gacgaaggtc
     1921 gcactcgaat ggcctcgctt ttccttgaag cgttgcttcc gtcacatcgt aaatgatgtc
     1981 ggtgccggct acgaacggcg caaatcgaat cgtcggcttt gcggctcgcg atgagtcgct
     2041 ccatgccaga ccgcgcgaga cgaacacgta ggttgcatcg tctccgccgc tgaagtgctc
     2101 gacagtgacc gagctttgga aagccgcatc gacgacgaac ggacactcgc catagtccgt
     2161 ccgcagattg tcgttcagga ggcgatatga ttcttcgtcc gcgctaggcg tatccatggt
     2221 ctttactccg gtgggatcgc ttaaagcccc tgctgcaagg ccgattgaat gaattcgttc
     2281 gattccgtcc cgacggacag ccggatcgtc ccgccagtga tccccagcgc ggcgcgctcg
     2341 tcggcgctta ggccgcggtg gctggtggtt tcggggtggc tgagtgtggt ggaaagttcg
     2401 ccgagcgagg ggcagaaggg gatttgcttc gcggcggcga tgaaggcgtc ggctgcgggt
     2461 cggccaccgg cgaagttaaa tgaaacaatc gtgccgtagc ggcccatgaa ttgtctcttc
     2521 gcaagtgcgt gctgcgcgtg gcttgacaga cccggataga agacgcgctc gatttgcttc
     2581 ttctgcgaat gcaggaattc ggcggccgca aacgcgttgt cgcaggcccg ctcgatccgc
     2641 agatgagccg tagccaggcc gcggtcagcc agccagcagt cgaacgggct gctcgaaagg
     2701 ccccaggcgg aaatcacggc gggcacgcgc tcgaagaccg cttcgcggcc gcacaaaagg
     2761 cccaggacca cgtcgctgtg gccgttcatc gtcttcgtca ggctttccat caccagatcc
     2821 gcacccagtt ccagcggccg gcagaggatc ggcgaggcga atgtgttgtc gatcaatagg
     2881 gcggctttgc gggagtgggc gatttccgcc agggcggcga tgtcggcgac ctccaacagc
     2941 ggattggcga tcgtttccgc cagcagcagc ttcgtattcg gcttgaatgc cgccgcggtt
     3001 gctttcaaat cgcacgtatc gaccgtcgtc atctcgatcc ccagccgcga ggcttccgcg
     3061 ccgaacagca cgtgcgtctt gccgtagaca cgcccgccaa caacgcaatg gtcgccggcc
     3121 ttcagttgcg aaagcagggc cgcggccagc gcgcccatgc cgctggccgc aattgccgcc
     3181 cggtccgcgc cgtgaagctg ccggcacttt tcggccagta gccaggcgtt gggatgcccg
     3241 tcgcgctgat agacatagcc cggctgttgg ccgccaagca tcgcgtcggc ctgctgcggc
     3301 gattcgcact cccagaccga ggcgggccag atcggcggcg cgtgcggcgt cgtcgccaag
     3361 ggcggcagcg gctgagggcg agggcagacg tcttccgggc ggggcattgc ggattgcgga
     3421 ttgcggattg cggattggcg gccaaggccc agtatatcgt gggccggaac gggtcgcttc
     3481 gtgtatgctg gtgggatgcg aatggaccgg cggcaattcg tggcgggagc aagtgcagcg
     3541 gctggctgcg cggcgatcgg tacgcgcgcc tttgccgcac catttgtgcc cgcccgtgat
     3601 ccgcaagtgg tggcgttcgc gccgggcggc acgatcatcg ccaccggctg ctcggggatg
     3661 accgacagca cgtttccgcc ccggccgcat ccggacgtgc ggaagtgcgg cgtcgtcgcc
     3721 gtctgggacg tggccacgcg aaagcggctc tttcgctggg agacctacgg cgacctgacg
     3781 aagctcgcct tttcgcccga cggcacgctg ctggccgcca gccggctgtt tgaaacgccc
     3841 gatgggctgg ccctgcacga agtccgcgtg tgggacgtga ccagcggccg ggtcgtcaag
     3901 tcgctcgatc gctgccacgc cttcgatttc gccccgatcg aaccgcaaat cgccgtcctc
     3961 agccgcagca agtgcgtcgt ctatgacctg caggactgga cgaaggagaa gctcgtcgcg
     4021 ccgctgggcg gagcggtttc gattgcattc acagccgacg gcctgtcgct gatcggcgtg
     4081 gtgaaggacg aggggaaata tcggctgcgg ctaacgagtg tcgaatctaa tgcggccggc
     4141 gagacgccta ccccacgtgg ggcaggcatc ctgcctgcca ctcaatcgca agcactcgac
     4201 gagcccttct atcgcgccgc ggtcgtggcc gatggctcgc tgctggcgac cggccatggc
     4261 ggcggcaacg tcgtgctgtg ggatgccgcg acgctcgacg tccgcggccg attgcagacc
     4321 ggcgttcgcg gcctggccca cccgttcttt tcccctgatg cgaccctgct cgcggccggc
     4381 tgccaggaaa acggcgacgt ggtgatctgg aacctccagc agcgacagga agccggccgt
     4441 ttcacattcg agaaaggcgg cctgcggacc tatatgcagc ggccagccaa cgcgaccttc
     4501 cgccccgaag aagacccgac gcggttctgc ttctctcccg gcggcgaagc gatcctcgtc
     4561 ggcgcctacg gcggcatcct ccgcgccacc agcggcggcc aagagctggg gcggtttggg
     4621 gattgatgca agaacgcggt tgaccaatag ataccaactg gtacagttga ggtaggccgc
     4681 ccggtcaccg cttatgaaac cgactcctga cctaccgaag aaccggcaga gaattcgccg
     4741 ggaacgaaaa ctgacggccg aggaaatcgc acaggtgcgc gcggctcggg ccgaatttgc
     4801 cggacgcccg tcgaaacgcg atctgctggc ctcaggcgat tacgttggtc cgatgagcct
     4861 ggaagaattc ttggaatggc gaaagggcgc cggcgatgcg cctttgacac ggcagcttca
     4921 agcggcgatt gcggctaccg gacaatcgct ctacgccgtc gcgcaagcca gcggcgtagc
     4981 ggcccccgtg ttgcagcggt tcatcaacgg cgaacgtggg attactctgg agacggctgg
     5041 caagctcgcg gcctatctgg gactttcgct gatgcccgaa tcgcgcccat gacgcttcag
     5101 tcgctcctcg gcgagcaacc cgtttctcgc ttcatcgccg agcactacca acgcctgccg
     5161 tactcacttg ccggcggcgc ggattcgtta agagaacttg gcacgtggga atcgcttacc
     5221 gcgatcctcg gccagcccgc tgccgacctg ctcgtctgcc gccgtaacga gcgttacgat
     5281 ggtcccgcgc cgcgaactgc ggacgatgcc cg
//
LOCUS       DASUGB010000039         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_5395,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000039 DASUGB010000000
VERSION     DASUGB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5395"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..550
                     /locus_tag="VFV87_00785"
     CDS             332..550
                     /locus_tag="VFV87_00785"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX5102314.1"
                     /translation="MPDHVPKPKIPFDTLAEQRIRAAQAAGEFDNLPGFGQPIPGLDE
                     PHDELWVKRLLTGRRGTVRGQAAAARRG"
     gene            complement(495..1613)
                     /gene="thiO"
                     /locus_tag="VFV87_00790"
     CDS             complement(495..1613)
                     /gene="thiO"
                     /locus_tag="VFV87_00790"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX5102315.1"
                     /translation="MHDVLIIGGGVIGLSLAWELVKRGKSVQLIDQGQPGQEASWAGA
                     GILPPAALRPGDHPLDQLRGLAYELHPRWAADLQEFTGIDTGYRRCGGLYLSQSPGES
                     AALAAWADTLRDEGIEVEKLSAADLSELEPSLRRTPSTEYSVLSTQYLLPEEGQLRNP
                     RHLKALVAACEKSGVTISPHIPASEFVVSKGELTELRTTAGALPARQFCFTAGAWTGH
                     LLQRLGIGAGILPIRGQMVLFRCQRPPITRIVNLGSRYLVPRDDGYVLAGSTEEEVGF
                     DKRTTEEAIADLTQFARDLAPALREATIERTWAGLRPGSFDGLPYLGPLPGLKNAFIA
                     AGHFRTGLFLSPATAYVMSQLIRGEQPQLDLAPFRVGR"
     gene            complement(1623..2300)
                     /locus_tag="VFV87_00795"
     CDS             complement(1623..2300)
                     /locus_tag="VFV87_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5102316.1"
                     /translation="MKSLLGMFAKLWEPGQVKTRLAASTGDNPAAEIHRAFVETLATR
                     LAGAADLCRLCYAPASAAEGFANFAQLGWQLTPQFAGDLGRRMRQFFETSLAAAERVV
                     LIGSDSPDLPAVYIAEAFESLRSCPVVLGPAADGGYYLIGASRQVPPVFEGIAWSTSN
                     VWRQTVERLQSAGMKWHELPVWYDVDDDQSLNALLLRLQSEGQTDHALADLRERLTAL
                     LNRRPAE"
BASE COUNT          475 a          743 c          771 g          434 t
ORIGIN      
        1 acggcatcag ccccgctcaa ggttgcaatt gagattggac ggtgccaatc aaatcgcatc
       61 aaagcgattc cagagatcct tacaaagttc ggtatcgagt ttgtcccatc ctctcgttac
      121 gaatggctag gaggagtcga agggcggtgc cgcgactgtg gtgcccaatt gcgcatgcca
      181 ccgcgcttcg aggaatatgg cggaaccatt cggtgcccga atggctgctt ttttatgcac
      241 gtaacaagga agccgccggc gacataggcg agtctccaaa gttcaatctg ctcgcacttc
      301 tccgggagca gatttgctcc cggctgagaa catgcccgat cacgttccca agccgaaaat
      361 cccattcgac acgctggcgg agcagcggat tcgggcggcg caggcggcgg gggagtttga
      421 caatctgccg ggctttgggc agccgatccc ggggctcgat gagccgcacg acgaactgtg
      481 ggtcaagcgg ttgcttaccg gccgacgcgg aacggtgcga ggtcaagctg cggctgctcg
      541 ccgcggatga gttggctcat gacataggca gtggcgggcg aaaggaacag gccagtgcgg
      601 aagtggccgg cggcgatgaa agcgttttta agaccgggta gtggaccgag ataagggagg
      661 ccgtcgaacg agccggggcg gaggccggcc caggtgcgct cgatcgttgc ttcacggagc
      721 gcgggggcca gatcgcgggc aaattgtgtc aaatcggcga ttgcctcctc ggttgtgcgc
      781 ttgtcgaagc cgacttcttc ttcggtcgag ccggccagga cataaccatc gtcgcgtggg
      841 acaaggtagc gggagccaag attgacgatc cgcgtgatgg gcggccgctg gcaccgaaaa
      901 aggaccatct ggccgcgaat cggcaggatt ccggcaccaa ttcccaaccg ctgcagcaaa
      961 tgcccggtcc aggcgccggc agtgaagcag aattggcgcg ccgggagcgc gcccgctgtg
     1021 gtccgcaact cggtaagttc acccttggaa acgacaaact cgctcgctgg gatatggggg
     1081 gagatggtaa ccccggattt ctcgcaggcc gcgaccagcg ccttgagatg ccgcggattg
     1141 cgtagttgtc cctcttcggg aagcaagtat tgagtactaa gtaccgaata ctcagtactc
     1201 ggggttcggc gcaagctcgg ttcaagttcg ctcagatctg ctgcggacaa cttttcaact
     1261 tcgatccctt cgtcccgcag cgtatcggcc caagctgcta gcgccgcgga ttcgccgggg
     1321 gattgcgaga gatacagccc gccgcagcgg cggtagcccg tgtcgatgcc ggtgaattct
     1381 tggagatcgg ccgcccaacg cggatggagt tcgtaggcca agccgcgtag ctgatcgagc
     1441 ggatggtcgc cggggcgcaa cgcggcgggt ggcagaattc ccgcgccggc ccagcttgct
     1501 tcctggccgg gctgaccctg gtcaatgagc tggacgcttt tgcctcgctt cactaactcc
     1561 caagccagcg aaaggccgat cacaccgcct ccgataatga gcacatcatg catgctttca
     1621 ggctactccg caggccggcg attgagcagg gcggtcaggc gctcgcgaag gtcggccagg
     1681 gcgtgatcgg tctgtccctc gctctgcaaa cggagcagca gcgcgtttag gctctggtca
     1741 tcgtccacgt cgtaccacac gggtaactcg tgccacttca ttcctgcaga ttgcaaacgt
     1801 tcgacggttt gccgccaaac attggaggtg ctccaggcga ttccctcgaa taccggggga
     1861 acctggcgcg aggcgccgat caggtaatag ccgccgtcgg ctgcgggccc cagaacgacg
     1921 gggcaagatc gcagcgattc gaaggcctcg gcgatataca ccgcaggcaa atcggggctg
     1981 tcggagccaa tcaggacgac ccgttcggca gcggcgagcg aagtttcgaa gaactgccgc
     2041 atccgccggc ccaagtcgcc cgcgaattgc ggcgtcaact gccatcccag ctgcgcgaag
     2101 ttcgcaaacc cctcagcggc cgaagccggc gcatagcaca agcggcagag gtccgcggca
     2161 cccgccagac gcgtcgcgag cgtctccacg aaggcccgat gaatctcagc tgccggattg
     2221 tcgcctgtgc tggcggccag gcgcgttttt acctgccccg gctcccagag tttggcgaac
     2281 atgcccagga gcgacttcac ccgctagccc gcctttccca ccagaatccg cttgagcagc
     2341 acttcgttca cccgcccgcg gaaccgctcc ttgccgtcga tttccaccac cggaacgcac
     2401 tcgttatacc gctcgcgcag ggc
//
LOCUS       DASUGB010000040         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1950853, whole genome shotgun sequence.
ACCESSION   DASUGB010000040 DASUGB010000000
VERSION     DASUGB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950853"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1336
                     /locus_tag="VFV87_00800"
     CDS             155..1336
                     /locus_tag="VFV87_00800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5102317.1"
                     /translation="MRCFAFHLMPWDRLPADFDTKYETAWTWLPNSIYDPQHGHTLYN
                     RYLDELCLADELGFYGVCVNEHHQNAYGTMPSPNLMGSILARQTKRVKIAVIGNALPL
                     YNPVTRVAEEFAMIDVISGGRLIAGLVVGGGPEYYSFSMNPTNARAMFQEGMDLVLRA
                     WTEPGPFEHYGEFWKLKYVNPWPRPIQQPHPTIWIPGAGSKETIELVAARRFGYMGIP
                     YFHIDFFQRNFDMFREAAKKSGYEASPYQLGWLCPIYVAETDEQAWKEYEKHIMYFAK
                     QLLKGLVVLPPGYTSVRSIAGIHTALSKFLSTVETRKQIEDGCYAIVGSPETVRQKLL
                     ENGKKLGVGNLLGLFQLGTLPADLTRKNMQLFAKEVMPALQAVEHAPLPAMAPAGVTC
                     N"
     gene            1457..2236
                     /locus_tag="VFV87_00805"
     CDS             1457..2236
                     /locus_tag="VFV87_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5102318.1"
                     /translation="MTKPTTEFPTIAGKKTQLTMGGSGPPLLYLHSAGGETEWMPFHE
                     KLAQHFTVYLPAHPGFADSKGFDEIRDIYDYAWHYVDFLTELKLGPVPVVGFSLGGWT
                     ALELAILRPALVQKIVLVDAAGLRLSEAPMAELFIDDLAKLRNLLFFDPNDPCVKIAM
                     PDSLDDPRIVQWLRAREATARVAWNPYLHNPRLPRHLRRVECPVLALWGKQDRLIPLA
                     HGEFYAKNIPGARLETIDNCGHMLPFEKPDEFVKRVVAFLK"
     gene            2233..2553
                     /locus_tag="VFV87_00810"
     CDS             2233..2553
                     /locus_tag="VFV87_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5102319.1"
                     /translation="MIGNSPRGRGGEAMSQRTERRRVHYSGRVQGVGFRFTVHRIAQS
                     HAVTGFVKNLDDGRVELVAEGEAAELDRFLIAIGEHMKRYIRAADTSTSPATGDFKLF
                     EIAY"
     gene            2773..4620
                     /locus_tag="VFV87_00815"
     CDS             2773..4620
                     /locus_tag="VFV87_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5102320.1"
                     /translation="MTQLFSPVLLADIFDRFRIQLTDGITNWLTPVWILCLGATVGLI
                     LCAAIWAVLWALSRLPGIGTLADSPTSRWIATAVLTVALFVVSLIVLPSARVPAANAA
                     DGANAAQEAPDSGLVEILSARGARALICALVATVIVTLVSRKALVETDIALREGVLFP
                     LAMVALAMTVFTLLGLVVVRRPTELLESLARYPALAIRGPVTTAKTIEAPPNLNDEPP
                     QLSIPVDFRRDEIAELTVESDQHVKFSSTPIDQPLVTALKFDLAAGESTTWKRQPGGI
                     NPFREQSIKQLYVQNFSTSPAEVKVTVVSQMAHPQMLLVPIMALGMAGIFLLYLFQRA
                     AMPKLAAVALSTSKSDIAQPLYVLVLAGGLFLLVVFVYIPYNTFGDDIKQLKDSGLNL
                     ILVLCILQAVWAASTSVADEVEGKTALTVLSKPVSRRDFILGKFVGISWSTGLLCVVL
                     GLLLLVTVAYKPIYDFREGGWESPDVKNADPTWQNCYNEMVQTTPGLVLVYMEALVMA
                     ALSVAISTRLPTLANFIICFAIYVLGHLTPLIVQSQVVANQLPPVVFFGRLIATVLPV
                     LDHFNIQASIAAGAEVPVGYLAWAFVYCLLYSTVAMLLALTLFEDRDLA"
     gene            complement(4858..>5616)
                     /locus_tag="VFV87_00820"
     CDS             complement(4858..>5616)
                     /locus_tag="VFV87_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX5102321.1"
                     /translation="VAVAWILKKTIFRGPTPPFVMELPSYKLPGVGIVLHRMFEQGWA
                     FICRAGTLILAVSILVWFLGYFPHDRSQIDPQLFAQQAELEQLAVDGQTSAEQAAELA
                     AVENQIAGAYLEHSILGRLGHFIEPAVRPLGWDWKIGCAAIASFPAREVVVATLGVIY
                     NLGDEEDESSDTLQEALQNATWHGTDRKVFNLPVALSIMVFFALCAQCASTLVIMRRE
                     TGSWRWPALTFVYMTTLAYVGALITYQVGIRLLG"
BASE COUNT         1063 a         1728 c         1747 g         1078 t
ORIGIN      
        1 gtgggtctgt aagactgggg cgggttggcg gttcggctga gttacgcctg cgaagaaaca
       61 taggcacccc aaaaccctcg cttacgcgtc gggttagtgt tgacgattgc agaacaaacc
      121 ttcacgggca ggcgagacgc ctaccccaca aaccatgcgt tgctttgcgt ttcacctgat
      181 gccttgggac cggctgccgg cggattttga tacgaagtat gagaccgcgt ggacctggct
      241 gccgaattcg atctacgacc cgcagcacgg gcacacgctt tacaaccgct atctggacga
      301 gctgtgcctg gccgatgagc tggggtttta cggcgtctgc gtcaacgagc accatcagaa
      361 cgcgtatggc acgatgccca gcccgaacct gatgggttcg atcctcgctc gccagacgaa
      421 gcgggtgaaa atcgcggtga tcggcaacgc cctgccgctc tataacccgg tgacgcgcgt
      481 cgccgaagag ttcgcgatga tcgacgtgat cagcggcggg cggctgatcg cggggctggt
      541 cgtcggcggc gggccggaat actacagctt ctcgatgaac ccgaccaacg cccgggccat
      601 gttccaggag gggatggatc tggtcctacg ggcgtggacc gagccgggac cgttcgagca
      661 ttacggcgag ttctggaagc tgaagtacgt caatccctgg ccgcggccga tccagcagcc
      721 gcacccgacc atctggattc ccggcgccgg cagcaaggag acgatcgaac tggtcgcggc
      781 ccgccgcttt ggctacatgg gcattccgta ctttcacatc gatttcttcc agcggaattt
      841 tgacatgttc cgcgaggcgg ccaagaagag cggctacgaa gcgagcccgt accagctcgg
      901 gtggctctgc ccgatctacg tagccgaaac cgacgagcag gcctggaagg aatacgagaa
      961 gcacatcatg tatttcgcca agcagcttct gaaggggctt gtcgtcctgc cgccgggata
     1021 tacgagcgtg cggtcgatcg ccggcatcca caccgcgctg tccaaattcc tcagcacggt
     1081 cgagacgcga aagcaaatcg aagacggctg ctacgcgatc gtcggcagcc ccgagacggt
     1141 caggcaaaag ctgctggaga acggcaagaa gctgggcgta ggcaacctgc tcggcctgtt
     1201 ccagctcggc acgctgccgg ccgatttgac gcgaaagaac atgcagctct tcgccaaaga
     1261 agtgatgccg gcgctgcaag cggtggagca cgccccgttg ccggcgatgg cgccggcggg
     1321 ggtgacgtgt aattgacaga actggttccc acgctctgcg tgggaacccc agccatggcg
     1381 ctctgcgtcg ccgcagggac gcggagcgtc cagctcgtgc gttcccacgc gaagcgtggg
     1441 aacgagattg aaatctatga cgaaaccaac gaccgaattt cccaccatcg ccggcaagaa
     1501 gacgcagctc acgatggggg gcagcgggcc gccgctgctc tatctgcatt cggcgggggg
     1561 cgagacggag tggatgccgt ttcacgagaa gctggcgcag cactttaccg tctatctgcc
     1621 ggcgcatccg ggctttgccg attccaaggg ctttgacgag atccgcgaca tctacgacta
     1681 cgcctggcac tacgtcgatt tcctgacaga gctaaagctc gggccggtgc cggtcgtcgg
     1741 cttctcgctc ggcgggtgga cggcgctgga gctggcaatc ctgcggccgg cgctcgtgca
     1801 gaagatcgtg ctggtggatg ctgccggctt gcggctgtct gaggcgccga tggcggaatt
     1861 gttcatcgac gacctggcca agctgcggaa cctgctgttt ttcgatccga atgacccgtg
     1921 cgtgaagatc gcgatgcccg attcgctcga cgatcctcgg atcgtgcaat ggctccgcgc
     1981 ccgcgaggcc actgcccgcg tcgcctggaa cccctacctg cacaaccccc ggctgccacg
     2041 gcacttgcgg cgcgtcgaat gcccggtgct cgccctctgg ggcaagcagg acaggctgat
     2101 tccgctggcc cacggcgagt tctatgcgaa gaacatcccc ggcgcccgtc tcgaaaccat
     2161 cgacaactgc ggccacatgc tcccctttga gaagccggat gagttcgtca agcgcgttgt
     2221 cgcgttcctc aagtgatcgg caattcgccg cgagggcgcg gcggtgaggc catgtctcag
     2281 cgaactgagc gccgccgagt tcattattcg ggccgggttc aaggcgtcgg ctttcggttc
     2341 accgtccacc gcatcgcgca aagccatgcg gtgacaggct ttgtgaaaaa tctcgacgac
     2401 ggccgagtgg aactggtcgc ggaaggcgag gctgccgagc tcgaccgatt tctcattgcc
     2461 atcggcgaac atatgaagcg ctatatccgc gcggccgaca cttcgaccag tccggcgact
     2521 ggcgatttca agttgttcga aatcgcttac taggcaacga cttgcgattg cgacgcggcg
     2581 aattgccaga agaccgcctg gaggcgaagc agcgccaagc accctcccca agttacacgc
     2641 ctcaactaga ctggaatcct tgaaccggcc cgctgccagc agcgtaaagg tcgcatcccg
     2701 taagtacttc cggctcctct ggcctacggc gcagtctcag ccgccgggcc gaatgtcgcc
     2761 cttctgccaa ccatgaccca gctcttttcc ccggtccttc tggccgacat tttcgaccgc
     2821 ttccgcattc agctcaccga tggcattacc aactggctca cgccggtctg gatcttgtgc
     2881 ctgggagcga ccgtcggcct catcctgtgc gccgcgattt gggccgtgct gtgggcgctg
     2941 tcgcgccttc caggcatcgg cacgctggcc gattcgccga cgtcgcgctg gatcgcaacg
     3001 gccgttctga ccgtcgcgct gtttgtcgtc tcgttgatcg tcctaccttc cgcgcgcgtt
     3061 ccagccgcga atgcggccga cggcgcgaac gcagcgcaag aggccccaga cagcggcctg
     3121 gttgagattc tgtccgcccg cggcgcccgc gcgctcatct gcgcgctggt ggcgacggtg
     3181 atcgtcaccc tcgtcagccg caaggccctt gtcgagacgg acatcgccct gcgcgaggga
     3241 gtgctgttcc cgctggcgat ggtggccctg gcaatgaccg tttttacgct gctggggctg
     3301 gtggtggtcc gccggccgac ggagttgctg gaaagcctgg cgcgatatcc cgcattggcg
     3361 attcgtgggc cggtcacaac ggcgaaaact atcgaagcgc cgccgaacct gaacgatgaa
     3421 cctccacaac tgtcgatccc ggtcgatttt cgccgggacg aaatcgcgga actgaccgtc
     3481 gagagcgatc aacacgtcaa gttttcgagt actccgatcg atcagccgtt ggtgacggcg
     3541 ctcaagttcg acctcgcggc gggagaatcg acaacgtgga agcggcagcc cggcggcatc
     3601 aatccgttcc gcgaacagtc gatcaagcag ctctacgtgc agaatttcag cacgtcgccc
     3661 gccgaggtca aggtgaccgt cgtcagccaa atggcccacc cccagatgct gctcgtgccc
     3721 atcatggcgc tggggatggc gggaattttc ttgctctacc tgtttcagcg ggcggcgatg
     3781 cccaagctgg ccgcggtcgc cctgtccact tcgaaatccg atatcgccca gccgctgtat
     3841 gtgctcgtcc tggcgggtgg tttgtttctc ctcgtggtgt tcgtctatat cccctacaac
     3901 acattcggcg acgatatcaa gcagctcaag gattccgggt tgaacttgat cctggttctg
     3961 tgcattctgc aggcggtgtg ggcggccagc acgtcggtgg ccgacgaagt ggaaggcaaa
     4021 acggccctca ccgtgctctc aaaacccgtc agccgtcgcg acttcatcct cggcaaattt
     4081 gtcggcatca gctggtccac gggattgctg tgcgtggtgc tcggactact gctgctggtg
     4141 acagtggcct acaagccgat ctatgacttc cgcgaaggcg gctgggaatc tcccgacgtg
     4201 aagaacgccg atcctacctg gcaaaactgc tacaacgaga tggtgcagac gacccccggc
     4261 cttgtgttgg tgtatatgga agcgctcgtc atggcggctt taagcgtggc gatttcaacg
     4321 cgcttgccga cgctggcgaa tttcatcatc tgctttgcaa tttacgtgtt ggggcacctc
     4381 acgcctttaa tcgtccaatc gcaagtggtg gcgaatcaat tgccgcccgt cgtcttcttc
     4441 ggcaggctga tcgcaacggt gctacccgtg ctggaccact tcaacatcca ggccagcatc
     4501 gcagccgggg ccgaggttcc cgttggctat ctggcatggg cgttcgttta ctgcctgctc
     4561 tacagcacgg tggcaatgct cctggcgcta acgctgttcg aggaccgcga tctggcatag
     4621 gtgcgctagg tgccgtgctc cgtttgatcg gctggctcgc tcgaaatctg cagaagagtt
     4681 cgcgctgcag agctgctggt tgggcaggag gagcaactgt tggagcaaac ggcattggga
     4741 ccgaaggcga acgtgcgcca cgcgcgaaag gcaacgtatg ctgccgccag caggcagatg
     4801 gggatcgtca gccaggtttg ccagtcggtc cacacgttcc aacgccaatt cgtaggctta
     4861 tccaagcagg cgaatgccaa cctggtaggt gatgagcgct ccgacatagg cgagcgtcgt
     4921 catatacaca aaggtcagtg ccggccaacg ccagctacct gtctcgcgtc gcatgataac
     4981 cagagttgag gcgcactgcg cacacagggc aaagaatacc atgatcgaaa gggcaaccgg
     5041 caagttaaat accttgcggt cggtgccgtg ccaggttgcg ttttgcaggg cctcttggag
     5101 cgtgtcggag gattcgtcct cctcgtcgcc gagattgtag atcacgccca gggtggccac
     5161 gacgacctcg cgtgccggga acgacgcaat cgccgcgcag ccgatcttcc agtcccaacc
     5221 gagcggccgc acggccggct cgatgaagtg gccgaggcga ccgaggatgc tgtgctccag
     5281 gtaagcgccg gcgatctggt tctcaactgc agccagctcg gcggcttgct cggcggaggt
     5341 ttgaccgtca acagcgagtt gctccagctc ggcttgctgg gcaaatagct gcggatcgat
     5401 ctgcgatcgg tcgtgcggaa aatagccgag aaaccagaca agaatcgaga cggcaaggat
     5461 cagcgtcccg gcgcggcaaa tgaaggccca gccctgctcg aacatccggt gcagtacgat
     5521 gccaacgccg gggagcttgt agctgggcaa ttccatgacg aacggcggcg tcggcccgcg
     5581 gaagatcgtc tttttcagaa tccacgccac ggcgac
//
LOCUS       DASUGB010000041         4884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1533922, whole genome shotgun sequence.
ACCESSION   DASUGB010000041 DASUGB010000000
VERSION     DASUGB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533922"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1049
                     /locus_tag="VFV87_00825"
     CDS             189..1049
                     /locus_tag="VFV87_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX5102322.1"
                     /translation="MRIVYGGSFALAVTLASVALAQNVREPARPATPATPRAGAAAEA
                     RADNADQQIASLLYHCCQNDIEISKFAKDRLQSEEARQFAEKMIAEHTEGCQKLAKFA
                     GAATGADTRLEAIPGEPRREGRREGRDEAPRPAPAAAPRAAAEGGRGIDVRAGAVEVQ
                     VQPGLRPRVDARGPGGQLDWIAIFQQISDQNAANFKKELAAKEGAELDQCYIGGQIMG
                     HMMAQSEVQVLRQHASPELRQELDACLQGCAAHLQEAKTLAKQLAASGTPRTARRPGA
                     PESPPAPKPE"
     gene            1282..4221
                     /locus_tag="VFV87_00830"
     CDS             1282..4221
                     /locus_tag="VFV87_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5102323.1"
                     /translation="MLFCRRILYCIVALSATALSAAEPLEISSPLTPDEAQKLFQLAD
                     TNLQIELAAAEPEVVDPVAIRFDEDGRMWVCEMRDYPLGPPPGTSKSSRIRVLEDTDG
                     DGRFEKATTFADGLSFVTGLQPWKGGVFVTLSGAVAYMKDTDGDGKCDLDETWFEGFA
                     EQNTQLRANHPKLALDNWIYVANGLRGGKVISRKLKEQGPIDISGMDFRFDPLTGKAE
                     AVSGNGQFGLTFDDWGNRFLCSNRNPCRHVVIEDRYLKANPGVTVAAVVHDVAAFAEN
                     SRIYPISRAWTTSNLHAGQFTAACGVYIYRGDLLPQEFYGNVFTCDPTGNLVHREIMQ
                     PAGPTFTSKPAYEGKEFLASPDEWFRPVNMELGPDGALYVVDMYRAVIEHPDWVPDEL
                     KRRPDQRLGDDRGRIWRIVGSDRRAEPEASTRRPSPKLSQADSDDLLPLLGHKSAWHR
                     ETAQRLLLERQRQALAPRLRELATSGDNSQARARALRLLSGQAQLEPQNVVSALEDAD
                     PCVRALAVQIVESFAEDSELRSKASGLVSDPDPAVRFQSRLSVRGGLNPFLHRSPDID
                     DPWMRVGILLAYKPETAALSMRKFFPSDASAPAPSRGWLSFLNQLAEYGMGSKVASVR
                     DLGIRAVVDELNDGNQPASALSALHGVVRGMARRGVPLSKIDMKEQSWSNVAERVAAI
                     ANGESTPLELRLVAIDVLGYLPATAAVLQPFCERERDQDLRIAALSSLARQGEIDLWH
                     SLIDTFASELPPVRRAIVDGMLANTVRTNLLLDAIEAAKIKPSELDAAHVTRLTQHRD
                     PAIKERAAKLFAAATPAERAKALADYQPVLKMNADAARGKAIFEKNCAACHKIGGIGT
                     NVAPDISDSRTKKLDQLLGDILLPNRAIDNNYIGYNVRLLDGTVTTGVLSAETSTSIT
                     LRQQGGKEAVIPRSEIEELKSSGLSLMPEGLEKQIPHQDMADLLAFIKNWRYLDGRTP
                     LGEPISR"
     gene            complement(4218..4466)
                     /locus_tag="VFV87_00835"
     CDS             complement(4218..4466)
                     /locus_tag="VFV87_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102324.1"
                     /translation="MIVGRIEDGQIRLIAPLPEAWEGQTVKVEPCTPDDVLPGLEQRL
                     AALHALGPMEYEGDERLEIERSLKAMDDLSRIQMQQHG"
     gene            complement(4509..>4884)
                     /locus_tag="VFV87_00840"
     CDS             complement(4509..>4884)
                     /locus_tag="VFV87_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX5102325.1"
                     /translation="YHWAAVRYRASHQGCKCELRTSGMEGCQLSLSAAFDSSAAKARS
                     GTRQAWLLERYRAFVPPLQAAVEHPPWSFQLARVELGENTLGCHLGMDLNRPPDEAHF
                     CREMQARFSRFSSCTRRVLNVP"
BASE COUNT          935 a         1563 c         1502 g          884 t
ORIGIN      
        1 tctgtagccg cgcttgccac aacgagagat tcccggcgtt tccgcacgct ggcaagagcg
       61 gctacgtgaa gaattgggtg ccgggaaggc ggcatcgaat gtgctgatga cggaataact
      121 tcaggcacat ctgcggtggc cccgcgtttg ggcgccatcg cacaccccgc gaactgagga
      181 gagttcaaat gcgcatcgtt tacggaggtt cgttcgccct tgccgtcacc ctggccagcg
      241 tggccctggc ccagaatgtc cgcgagccgg cccgccccgc aacaccagcg acgccccgcg
      301 ccggcgcagc ggccgaggcc cgggctgaca acgccgatca gcaaatcgct tcgctcttgt
      361 atcactgctg tcaaaacgac attgagatct ccaaatttgc caaggatcgt ctgcaatcgg
      421 aggaagctcg tcagttcgcc gaaaaaatga tcgccgagca caccgagggc tgccagaagc
      481 tggcgaaatt tgccggcgct gcgactggcg ccgatactcg cctggaagcc attccgggcg
      541 agccgcggcg cgaaggccgc cgggagggac gtgatgaagc gccgcgtccc gccccggctg
      601 ccgcgccgcg agcggctgcg gaaggcggcc gcggcattga cgtccgcgcc ggcgccgtcg
      661 aagtccaagt gcagcctggg ctgcgtccgc gagtggatgc ccgtggtccc ggcggccaac
      721 tcgactggat tgcgatcttc cagcagatct ccgaccagaa tgccgccaac ttcaagaaag
      781 agctggccgc gaaggagggt gctgagctcg accagtgcta catcggcgga caaatcatgg
      841 gccacatgat ggcacagagc gaagtccagg ttctccgcca gcatgcttcg cccgaattgc
      901 ggcaagagtt ggacgcctgc ctgcagggct gtgcggcgca cctccaggaa gcgaagactc
      961 tcgccaaaca gctcgcagcc agcggtacgc cgcgcaccgc ccgccgtcca ggcgcgccgg
     1021 agtcgccgcc cgccccaaaa ccggagtaag gaggcttatc tccacggagg gtcaacgagg
     1081 agcgggccgg ctggatagga gcatccagcc ggcccgtctt tgtttgggtt cgggcgttca
     1141 ggattcaggc ggagacgctc ctgatcttgc tcgtcgtctt aatcctcttc ctgatctttc
     1201 tcttgctatt gctcctacgc ttgcagccct gcgtccgcat tacaatggca gcatcaccca
     1261 cccacctggg agaagctgcc gatgctgttc tgccgccgca ttctgtactg tattgtcgcc
     1321 ctaagcgcga ccgctctttc tgccgcagaa ccgctggaga tcagcagccc gctgacgccg
     1381 gacgaggccc aaaagctctt ccagctcgcc gacaccaatc tccagatcga gctcgccgcc
     1441 gcggagccgg aggtggtcga tccggtcgcg atccgctttg acgaggacgg gcggatgtgg
     1501 gtttgcgaga tgcgcgacta tccgctcggc cccccgccgg gtacgtcgaa atcgtcgcgg
     1561 attcgcgtcc tggaagatac cgacggcgac gggcggttcg agaaggcgac gacgtttgcc
     1621 gatggcctct cgttcgtcac cggcctgcag ccgtggaagg ggggcgtgtt cgtcacgctc
     1681 tccggcgcgg tcgcctatat gaaggacacc gacggcgacg gcaagtgcga cctcgatgaa
     1741 acctggttcg agggctttgc cgagcagaac acgcagcttc gggcgaacca cccgaagctg
     1801 gccctggata actggattta tgtcgccaat ggcctccgcg gcggcaaggt gatcagccgc
     1861 aagctgaagg agcaaggccc gatcgacatc agcgggatgg attttcgctt cgatccgctc
     1921 acgggcaagg ccgaggcggt cagcggcaac gggcagttcg gcctgacgtt cgacgactgg
     1981 ggcaaccgct tcctctgcag caatcgcaat ccgtgccggc atgtcgttat cgaagatcga
     2041 tatctgaagg ccaatccggg cgtgaccgtg gccgcggtcg tgcacgatgt cgccgccttc
     2101 gccgaaaaca gccgcattta ccccatcagc cgggcctgga cgacctcgaa cctgcacgcc
     2161 ggccagttca cggccgcctg cggcgtatat atataccgcg gcgatttgct cccccaggag
     2221 ttctacggca acgttttcac gtgcgacccg accggcaatc tcgtgcatcg cgagatcatg
     2281 caacccgccg ggccgacgtt caccagcaag ccggcttacg aaggcaaaga gttcctcgcc
     2341 tcgcccgacg aatggttccg gccggtcaat atggagctgg gtcccgacgg cgcgctgtac
     2401 gtcgtcgata tgtaccgcgc cgtcatcgaa caccccgact gggttcccga cgagctgaag
     2461 cgccgccccg accagcgcct cggcgacgac cgcggccgca tctggcggat cgtaggttcc
     2521 gaccgccggg cggaaccaga agcgtcaacc cgccgtccat caccaaaact ctcgcaagcc
     2581 gacagcgacg acctcctccc cctcctcggc cataagagcg cctggcaccg cgaaacggcc
     2641 cagcgcctct tgctggaacg ccagcgacaa gccctagcgc cgcggctgcg cgaactcgcc
     2701 acctccggcg acaattcgca ggcacgcgcc cgtgcccttc ggctcctttc ggggcaggcc
     2761 cagcttgagc cacaaaatgt cgtctctgca ttggaagacg ctgatccttg cgtccgcgct
     2821 ctggctgtgc agatcgtgga atcgttcgcg gaagacagcg aactcagatc aaaggcctca
     2881 ggactagtga gtgatccgga tccagcagtg cggtttcagt cgagattatc agtgcgaggc
     2941 ggactaaatc cgtttcttca ccgttctccc gatatcgatg acccgtggat gcgagtcgga
     3001 attctgctcg catataagcc ggaaacggcc gcattgagca tgcgaaagtt cttcccatcg
     3061 gatgcaagtg ctcctgcgcc gtcgcgcggt tggttgtcat tcctgaatca gcttgcggaa
     3121 tacggaatgg ggtccaaggt cgcatctgtc cgcgacctcg gaattcgggc ggtcgtagat
     3181 gagttgaacg acggaaatca gccggcctca gcgctatcgg cgctgcatgg tgtggttcga
     3241 ggaatggcac gtcgtggtgt cccgctgtcc aagatcgata tgaaggagca atcgtggtcg
     3301 aatgttgccg agcgagttgc cgcgattgcg aacggcgagt caacgccgct ggaactacgc
     3361 cttgtcgcga tcgatgtctt ggggtatctc ccggcgactg ccgctgtgct tcagccattc
     3421 tgtgagcgcg agcgtgacca ggacttgcgg atcgctgcat tgagttccct ggcacgtcag
     3481 ggcgagattg atctgtggca ttccttgatc gacacctttg cgtctgaatt gccgccagtt
     3541 cgccgagcga tcgtggacgg gatgttggcg aacacggtgc ggacaaacct cctcctcgac
     3601 gccatcgaag ccgccaagat caagccgagt gagttggatg cggcccatgt gacgcgattg
     3661 acgcagcatc gggatccggc gatcaaggaa cgggcggcca agctgtttgc cgcggcgacg
     3721 ccggcggagc gggccaaggc gctggccgat tatcagccgg tgctgaagat gaacgccgat
     3781 gccgcgcggg ggaaggcgat ctttgagaag aactgtgcgg cttgtcacaa gatcggcgga
     3841 attgggacga acgtcgcgcc agacatcagc gacagcagga ccaagaagct cgatcaactt
     3901 ctgggcgaca tcctgctgcc gaaccgggcg atcgacaaca actacatcgg ctacaacgtg
     3961 cggctgctgg acgggacggt tacgaccggc gttctttcgg ccgaaaccag cacgtcgatc
     4021 acgctccgcc agcaaggggg taaggaagcg gtcattccgc ggagcgagat cgaagagctg
     4081 aaatcgagcg gcctctcgct gatgcccgaa gggctcgaaa agcagatccc ccaccaggat
     4141 atggccgacc tgctcgcctt catcaaaaac tggcgctacc tcgacggccg cacgccgctc
     4201 ggcgagccga tttcgcgcta accgtgttgc tgcatttgaa ttcgactgag atcgtccatc
     4261 gctttcagcg accgttcgat ctccaaccgc tcatcacctt cgtattccat cggccccagg
     4321 gcgtgcaacg cggcaagtcg ctgctccagg ccgggcaaaa cgtcatccgg tgtgcaaggt
     4381 tcgaccttga cggtctgccc ttcccatgcc tccgggagtg gcgcaataag ccgaatttgc
     4441 ccgtcttcga ttcgtccgac aatcatgatc aagttcctcg tacgcaccat tatacgtggt
     4501 gctggtgctt acggcacgtt tagcacccgc ctcgtgcaag aactgaaccg gctgaaccgg
     4561 gcctgcattt cccggcaaaa gtgcgcctcg tccggtggcc ggttgagatc catgcccaag
     4621 tggcagccca acgtgttttc gccgagttcc acccgcgcta gttggaagct ccacggcggg
     4681 tgttcgaccg ctgcttgcag cggcggaacg aaagcccggt agcgctccag cagccacgct
     4741 tgccgcgtcc cgctccgggc cttcgctgcg gacgagtcga aggcggcgct caatgataac
     4801 tggcagcctt ccataccgct ggtccgcagt tcacatttgc agccctggtg cgacgcccgg
     4861 tagcgaacgg ccgcccaatg atac
//
LOCUS       DASUGB010000042         5409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1117306, whole genome shotgun sequence.
ACCESSION   DASUGB010000042 DASUGB010000000
VERSION     DASUGB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117306"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFV87_00845"
     CDS             complement(<1..868)
                     /locus_tag="VFV87_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5102326.1"
                     /translation="MAALLGVSVAAIATGLIPAGPRPTAAVEARLVAQAAGPSTNDRM
                     VTKIVTTLMKRDHLSKHPLDDEISQRALDLFVESLDGMKLYFYQSDIDEFNQRRKDLD
                     DMVAAGDVSFAYTVFNRLLKRVDERVAMVDELLKEDFDFTADETLVTDPKKLAYPANA
                     EEAKARWRKRLKYDMLVLKVDKTAAKEDPKERLARRYHSFAKRMHQTDSNELLEMYLT
                     AVTTSFDPHSTYMAPVSAANFQIILGLQLEGIGAQLRMNDGYTVIDKIIAGGAADKQG
                     DLKVGDRIASVGQ"
     gene            complement(1039..1938)
                     /locus_tag="VFV87_00850"
     CDS             complement(1039..1938)
                     /locus_tag="VFV87_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEX5102327.1"
                     /translation="MSDRDSKIGVLLVGHGTASEVGTRQFLALAGQLTERVAPLPVEP
                     AFLEMRQPDIAAGLCRLIERGAAHHLAVPLLLFGAGHAKRDIPAAVYNAIRTLLAEAF
                     SRQPEPSRIAKAQWSGRNAFFLASQADHLGCHRAMVELSRQRYREAVAGLSPVPPEQT
                     ALVLVGRGSRNESATAEMHEFAQLRHQPAVEGDIFVSFLAMTQPSLAEMLPLVAARRF
                     RRVVVQPHLLFEGDLAENIRRAVSEIQSQHPDQEWLITPLLADGLETRPTAQDSPLRG
                     NELLLRVLEDRIQAAIVAAGVGD"
     gene            2116..3945
                     /locus_tag="VFV87_00855"
     CDS             2116..3945
                     /locus_tag="VFV87_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5102328.1"
                     /translation="MYSFLVMTGGARVGSHFVLAGDRQNRIGRGLECDVILTDPLSSR
                     IHAIITCEDGDWWVRDAGSRNGSFVNGQKIDEARLTHGTAIKIGSSEFEFRQAASRPS
                     DSVLLDHTQTLIRDRLVLQHDSGQFGLEALRDPERAHDFLTLHQLSVRLLGCTDPAEV
                     VQVSLDLLRDRARASVAGFLWASDDGQLKPQLVIPEDAAGKVQLSSDLTEMVIRQGKA
                     VWIDDQKSETSSAGLLHFADAICVPLVQAGKTLGALHLYLEQGRFRQSDFEIAISLAN
                     ILTVALTRARREAMLQAEQKRLADKSAVTGELLGDSKPMDDLKSRITRIARATGCVLV
                     RGESGTGKELVARAVHRASARSDRPMLSVNCAAIPRDLMESQLFGHKKGSFTGADADH
                     VGLFQQAHTGTLFLDEVGEMTLDGQAKLLRILEGHPFLPVGSTKEVSADVRVIAATNR
                     DLREFVKGGRFREDLYYRLSVFELYIPPLRERGSDIELLVNHFLEHFKRQHGRPNLAL
                     SPEARTKLLGYAWPGNVRQLRNVVDSAVVMSEGSQILPDDLGLRDAAGGEQLESLRMD
                     YWERKLIQEALKRTAGSVPEAAKLLGLGRATLYRKIEEHGIER"
     gene            complement(4741..4989)
                     /locus_tag="VFV87_00860"
     CDS             complement(4741..4989)
                     /locus_tag="VFV87_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102329.1"
                     /translation="MTTAGDALPHKTPRSPNPGDWVAFRPQAPQNPLAVASSLRELEG
                     ILVRLGIGLCEVEVYQESTMDASPFVGGGALELESSGS"
BASE COUNT          985 a         1823 c         1619 g          982 t
ORIGIN      
        1 cctggccaac gctcgcgatc cggtcgccga ccttcaaatc gccctgcttg tcggcagcgc
       61 cgccggcgat gatcttgtcg atcaccgtgt agccgtcgtt catgcggagc tgcgccccga
      121 tgccttccag ctgcaggccc aggatgatct ggaaattggc ggccgacacg ggcgccatgt
      181 aggtgctgtg cggatcgaag ctggtggtaa cggccgttaa gtacatttcc agcagctcgt
      241 tgctgtcggt ctggtgcatc cgcttggcaa agctgtgata gcgccgcgca agccgctcct
      301 tgggatcctc cttggcggcc gttttatcga ctttgagcac gagcatgtcg tacttcagcc
      361 gcttgcgcca gcgggccttc gcctcttccg cgttggcggg ataggcgagc tttttgggat
      421 cggtgaccag cgtctcgtcg gcggtgaagt cgaaatcttc cttgagcaat tcatcgacca
      481 tggccactcg ctcatcgact cgcttcagca ggcggttgaa tacggtatag gcaaagctca
      541 catcgcccgc ggcgaccatg tcgtccaggt ccttgcggcg ctggttgaac tcgtcgatgt
      601 ccgactgata gaagtagagc ttcatcccgt ccaggctctc gacgaacaag tcgagggccc
      661 gctgcgagat ttcgtcgtcc agcgggtgct tcgaaaggtg gtcccgcttc atcagcgtgg
      721 tgacaatctt ggtcaccatc cggtcgttag tgctcgggcc ggcagcttga gccaccaggc
      781 gggcttcgac ggcggcggtc ggccgcgggc cggcgggaat gaggcccgtg gcaatcgcgg
      841 caacgctcac ccccaggagc gcggccaaga agaaattccg ggggctacag gccgacgcga
      901 gcagatggcg cagtccacgc attccattcc ctttgacagt tagaagctcg agctttgggc
      961 ggcgcaaaat ccgtgcgccc aatactttac gcatattatg ccccggctga agccgaagca
     1021 agcaaacgcg tcggaattct aatcgccgac cccggcggcg acaatcgcag cttggatgcg
     1081 gtcctctagc actcgcagca gcaattcgtt gccgcgaagg ggcgaatctt gtgccgtagg
     1141 acgggtttcc aacccgtccg ccaagagggg tgtgatcagc cactcttggt ccggatgctg
     1201 cgactgaatc tcgcttaccg cgcgcctgat gttttccgcc aaatctccct caaacagcaa
     1261 gtgcggctgc acaacgacgc gccggaatct ccgagcggcc actagcggca gcatttccgc
     1321 cagggatggc tgcgtcatcg ccaggaaact aacgaagatg tcaccttcca ccgctggttg
     1381 gtgtcgcaac tgggcgaact cgtgcatctc ggcggtcgcc gattcgttgc ggctgccgcg
     1441 cccgaccagc accagtgccg tctgctctgg cggcacaggt gacaagcctg ccaccgcctc
     1501 ccgatatcgc tggcgagaca actcgaccat cgcccgatgg cagccaagat ggtcggcttg
     1561 actcgccagg aagaacgcat tgcggccgct ccactgcgct ttggcaatgc gggaaggttc
     1621 cggctgacgg gagaaggctt cggcgagaag cgtccttatg gcattgtaaa cagccgcggg
     1681 aatgtcgcgt ttcgcatggc ccgctccaaa cagcagcagc ggcacggcaa ggtggtgcgc
     1741 tgccccgcgc tcgatcaggc ggcatagacc cgccgcaata tccggctgcc gcatctccaa
     1801 aaacgccggt tcaacgggca agggcgcaac ccgctccgtc agttgcccag ccagcgccag
     1861 gaactggcgc gtgccgacct cgctggccgt gccgtggccg acgagcagga cgccgatttt
     1921 cgaatcgcgg tcagacatga gaagttgtag ccgcgttcgc cagaacgcgg gtcaggtccg
     1981 acgtgttcca cccgcactct ggcgagtgcg actccggcaa cccgagcgat tgccgtggcg
     2041 atgacgcgtg acgccatatt atattggcca cagggccagc ggcgttcttt ctccagcctg
     2101 cacgggaagc agacggtgta cagctttctt gtgatgaccg gcggtgctcg cgtggggtcg
     2161 cacttcgtcc tcgccggcga ccgccagaac cggatcggcc gcggcctgga atgtgacgtc
     2221 atcctcaccg acccgctcag ctcgcgaatt cacgccatca tcacctgcga agacggcgat
     2281 tggtgggtcc gcgacgccgg cagccgcaac ggaagcttcg tcaacggcca gaagatcgac
     2341 gaggcccgcc tgacgcacgg caccgccatc aagatcggca gctctgaatt cgaattccgc
     2401 caggccgcca gccggcccag cgacagcgtc ctgctcgacc acacgcagac gctcatccgc
     2461 gaccgcctcg tcctgcagca cgattccggc cagttcggcc tggaagccct ccgcgatccc
     2521 gagcgggccc acgatttcct cacgctccac cagctcagcg tccgcctgct cggctgcacc
     2581 gacccggccg aggtcgtgca ggtttcgctc gacctgttgc gcgatcgcgc gcgggcctcg
     2641 gtcgccggct tcctgtgggc cagcgacgac ggccagctca agccgcaact cgtcattccg
     2701 gaagacgccg cgggcaaagt ccagctcagc agcgatctga ccgaaatggt catccgccag
     2761 ggcaaagcgg tctggatcga cgaccagaag tctgaaacct ccagtgccgg cctgctgcat
     2821 ttcgccgacg cgatctgcgt gccgctggtc caagccggca aaacgctcgg cgctctgcac
     2881 ctgtacctgg aacaaggccg attccgacag agcgatttcg agatcgcgat ctccctggcg
     2941 aacatcctga cggtcgcact gacccgcgcc cgccgcgagg cgatgctcca ggctgagcaa
     3001 aaacgcctcg cggacaaatc cgccgtcaca ggcgagctgc tgggcgacag caagccgatg
     3061 gacgacctca aatcgcggat cacgcggatc gcccgggcca ccggctgcgt gctggtgcgc
     3121 ggcgaaagcg gcaccggcaa ggagctggtc gcccgggccg tgcaccgggc cagcgcccgc
     3181 agcgaccggc cgatgctcag cgtcaactgt gcggcgattc cccgcgatct gatggagagc
     3241 cagctcttcg gccacaagaa aggctcgttc accggcgccg atgccgacca cgtcggcctc
     3301 ttccagcagg cccacaccgg cacgctgttc ctcgacgagg tcggcgaaat gaccctcgac
     3361 ggccaggcca agttgctgcg aatcctcgaa ggccatccct tcctgcccgt cggctcgacg
     3421 aaggaagtct ccgccgatgt gcgggtcatc gccgcgacca accgcgacct gcgtgaattc
     3481 gtcaagggcg gccgcttccg cgaggatctc tactaccgcc tgagcgtctt cgagctctac
     3541 atcccgccgc tccgcgaacg ggggagcgac atcgagctgc tcgtcaatca cttcctggag
     3601 cacttcaagc gccagcacgg ccggccgaac ctcgctctct cgcccgaagc ccgcaccaag
     3661 ctgctgggct acgcctggcc cggcaacgtg cggcagcttc gcaatgtggt cgatagcgcc
     3721 gtcgtgatga gcgaaggctc gcagatcctg ccggacgacc tcggcctccg cgacgccgcc
     3781 ggcggcgagc aactcgaatc gctccgcatg gactactggg aacgcaagct gatccaggaa
     3841 gccctcaaac gcaccgccgg cagcgtcccc gaagccgcca aactcctcgg cctcggccgc
     3901 gccacgctct accgcaaaat tgaagagcac gggatcgagc gctaagtcac cgtctcgcca
     3961 agtagccgtc tcgctccgca agacgagcgc ccggccttgg agcaagatcg atcgcgggtt
     4021 cctctgggtt cctcttctct gtttctgtca taggcgtgcg gagacaatcg cgagcagttt
     4081 tcgacgcatc cgccgtcgac ctggggcggg ttctgagtca ccgggattct tcggacggcg
     4141 tcaatcatta ttgcctcgtc aataacgaga gggtccgcta gctccagata ctctgcttgg
     4201 cacgcgagaa aaacagccca ctttcggatc ctgttccggt atgtcgcgtc ccaaaggcga
     4261 atggcgagcg cgggatctgc tttccaatcg ccacgcgctt tgatttcttt tggtggattg
     4321 ccgttcgtaa gctcgagcgt cttgggttcg tttccatgta caaagcaatt acttgggagt
     4381 gtcagcgctt ggctggcgta ccagtcagca gcgtccgcgt gcgaatcaaa gcgacggctc
     4441 acgcgtcgca cggccacaaa ccgccacttg agctttctct tgacagcaaa gtacacaatc
     4501 cgatcgccct ctcggagtcg cggtgcgaag tttcctgcgc ggcttatgca ctcccaccac
     4561 cacccgcgga cgacgcctgg ctggcctccg ggttcgaatg tgcgcggcca tcttcgacta
     4621 cctcctggga aagagaacgg gacatccgtt cgattcccag caacgtagcc ggcagctaca
     4681 cttcccagag atttcttggg gaagagacac ataccagagg aacccgtgct tggccgagac
     4741 ctagctcccg gacgactcta actcgagcgc accgccgccg acaaacggcg acgcgtccat
     4801 ggtcgactct tgatacacct cgacctcgca caggccgatt cccagccgca cgagaatgcc
     4861 ttcaagttcc cggagcgacg atgcgaccgc gagcggattc tgtggagcct gcggtcggaa
     4921 agcaacccaa tccccaggat tcggcgaacg cggggtcttg tggggcaacg cgtcaccggc
     4981 ggtcgtcatc ggtcctccgc cttccgatgg atgggctagc actgtactat cttagcggca
     5041 cggcaaaagc actattgcgc ccggcgcgct tcgccccgcg gaaatctggg caaaccagcc
     5101 tatctgtgcc gggcgttcgc cacgcatttt cggcgtccgc gtgaagattt ccgccgcggg
     5161 gcttgaacgg aggtcgcggt ctgcgggtat aaaagttggc tgcccgcttt tctcgctgga
     5221 ccggttactg cccccatgtc gctctccttc ccgcacttcc gtccgcatcc gctctttcgc
     5281 ggcgggcatt tgcagacgat cttcggggcc tacctgccgt ggcgcaagtt cgcctaccgt
     5341 gcgacgctgc accgcgtccc gctccccgac ggcgaccaca tcgcactcca cgacgacctg
     5401 ccgacttct
//
LOCUS       DASUGB010000043         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1673092, whole genome shotgun sequence.
ACCESSION   DASUGB010000043 DASUGB010000000
VERSION     DASUGB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673092"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1456)
                     /gene="clpX"
                     /locus_tag="VFV87_00865"
     CDS             complement(167..1456)
                     /gene="clpX"
                     /locus_tag="VFV87_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5102330.1"
                     /translation="MPAGKDLSGGRRGASTTKKNAFCSFCRKSYRDVGPLVEGPGDVY
                     ICGECIDLCQSILEQEQRRRGSTKPLFNRIPTPREIVTQLDQYVVGQSQAKKVLAVAV
                     HNHYKRLSLGFEGGDVDIEKSNILLIGPTGSGKTLLARTLARVLNVPFAIGDATTLTE
                     AGYVGEDVENLLLKLLHAADFDLEAAQRGVLYIDEIDKIGKTSHNVSITRDVSGEGVQ
                     QALLKMLEGTIANVPPQGGRKHPEQQYIQMDTTNILFICGGTFTGIEDIVRKRLGKKT
                     MGFGSTCGTKNEQDLTELLPLVDTEDILEFGMIPELIGRLPVISSLAPLDLAGLVRVL
                     TEPKNALLKQYQTLFEMEDCHLEFSDSALRAIAGKALEKKTGARGLRSIVEGVMTDIM
                     FELPDQPKGSKYVIDEDVVLKRKPLFHVVSEPALKSA"
     gene            complement(1756..2019)
                     /locus_tag="VFV87_00870"
     CDS             complement(1756..2019)
                     /locus_tag="VFV87_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102331.1"
                     /translation="MGHVSLETWIQLLIWVAVLIGLVIVALVVVQRFRGSAAGKGANS
                     AELLTNFQEMRERGDISDADYRKIKSVLGDKIQDERSGERPTT"
BASE COUNT          442 a          819 c          762 g          537 t
ORIGIN      
        1 caacccggcg ccgaagcgcc cgagctggat gaagcactcg actacctccg cgagctctcg
       61 cggacgtttg gtgcttagcc gggaccaaac ctggtcccgg agaactgcga gttgacatca
      121 acccgcggcg cgccgggagc aagttttcta ccggctagga acagccctac gcactcttca
      181 gcgccggttc gctcacgacg tggaacagcg gcttgcgctt gagcacgacg tcttcgtcga
      241 tcacgtattt gctgcccttg ggctggtcgg gcagctcgaa catgatgtcg gtcatcacgc
      301 cttcgacgat cgaccgcagg ccacgggcgc ccgttttctt ttccagcgct ttgccggcga
      361 tggcccgcag ggcgctgtcg gaaaactcca ggtggcagtc ttccatctcg aacagcgtct
      421 gatactgctt gagcagggcg ttcttcggct cggtgagcac gcgcaccagg cccgccaggt
      481 cgagcggggc cagcgacgag atcaccggca gccggccgat cagctcgggg atcatgccga
      541 actcgaggat gtcctcggta tcgaccagcg gcaagagctc ggtcagatcc tgctcgttct
      601 tggtgccgca ggtggagccg aagcccattg tctttttgcc gaggcgcttg cggacgatgt
      661 cttcgatgcc ggtgaaggtt ccgccgcaga tgaagaggat gttggtcgta tccatctgga
      721 tgtactgctg ctcgggatgc ttgcggcctc cctgcggcgg cacattggcg atggtgcctt
      781 cgagcatttt gagcagcgcc tgctgcacgc cttcgcccga gacgtcgcgg gtgatgctga
      841 cgttgtggct cgtcttgccg atcttgtcga tctcgtcgat gtacagcacg ccgcgctggg
      901 ccgcttccag gtcgaaatca gccgcgtgca gcagcttgag gaggaggttc tcgacgtctt
      961 cgcccacgta gcccgcttcg gtgagcgtgg tggcgtcgcc gatggcgaag ggcacgttga
     1021 gcacgcgggc cagcgtgcgg gctagcagcg tcttgcccga gccggtcggc ccgatcagga
     1081 ggatgttcga cttctcgata tcgacgtcgc ccccctcaaa gccgagcgag agtcgcttgt
     1141 agtgattatg gacggcgacg gccagcactt ttttggcctg cgactgaccg acgacgtact
     1201 gatcgagctg ggtgacgatt tcgcgcggcg tgggaatgcg gttgaagagc ggcttggtgc
     1261 tgccgcggcg gcgctgctcc tgctcgagga tggattggca aaggtcgatg cattcgccgc
     1321 agatatagac gtcgccgggc ccctcgacga gtgggccgac gtcgcgatag cttttgcggc
     1381 agaacgagca gaaggcgttc tttttggtgg tgctggcgcc gcgacgccct ccgctcagat
     1441 cttttcctgc gggcatggta actccttagt gccccgctgc ctggaactac gctgtttcag
     1501 gcggcggcga aatctcaggg tgctcaaagg ccaacgttgc tgctcgaagg cgagccgcag
     1561 gcgcaaaagc gccggcgcgg ccactttggg gcatccctgt ccgcaagatc gtcgagcgta
     1621 ctatcacacc atcggtgctg ccagcctccc attcctagcg ggcgggccgc gggcgacaca
     1681 accgtcacac accggtcagg cttgcccgcc ccgcacagcc gccgtggacg aaccggccgc
     1741 tggccgccgc tgccgtcagg ttgtgggccg ctcgccggag cgctcgtcct ggattttgtc
     1801 tcccagcacc gactttattt ttcggtagtc cgcgtcgcta atgtcaccgc gctcgcgcat
     1861 ttcctggaag ttcgtgagca gttccgcgga gttggccccc ttgccggcgg cactaccgcg
     1921 aaacctttgc acgactacca gcgcgacgat gaccaacccg atcaggacgg ccacccagat
     1981 gagcagttgg atccacgttt ccagcgacac gtgacccatg gttcccgctc gatccggcgc
     2041 tcgcaaactc tcgctgacgc attcctgcac ttcattatgc agctttggaa tgatcagtcc
     2101 agtttaacgc gcgcactccc ggcggctaac ctgcttccgc gggccaactt cgcgccgttt
     2161 tccacccatg gaaaacctgg ctgccgcccc tccctgacag cagactacct gccggccgca
     2221 gcggtcagaa ccattccagc ctcctcgtcg ctgcgaccaa gcgtcaaatc cctacggacg
     2281 cctgccgcca agtgttcgcg cgagattcag ctcagctcga cgatttaacc gcgaccccgc
     2341 cgtcacccct cttttggtaa actgaacagt cccgaatcgc ggcgcctatc gtgtatgaaa
     2401 ctccacaatt gttaaacgcc aatatcgctt taattacctg tgccactgag tcttacggcg
     2461 atttatacgt tatgccttta ttgcacgtta ttttcctgcc tcgatatcac ctgaacacgc
     2521 cctaaagagg tgatattatt ccgtgtcaga ttcaatcatc
//
LOCUS       DASUGB010000044         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_978791, whole genome shotgun sequence.
ACCESSION   DASUGB010000044 DASUGB010000000
VERSION     DASUGB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978791"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFV87_00875"
     CDS             <1..1215
                     /locus_tag="VFV87_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5102332.1"
                     /translation="WSLRPQETTIAHILRSAGYQCGHFGKWHLGPVKADSPTNPGAMG
                     FHEWVSHDNFFELNPSLSRNGGPPEVIKGESSEIVVKEAIRFIDGAKRAGKPFLAVVW
                     FGSPHEPYSGLPEDLALYDDLPAKYTRLVGLTSNETGGPTRRPQGEVLRERYAEITAM
                     DRAIGHLRKHLADTGLRDDTLLFYCGDNGTSADSALGEPHRGVKGQVYEGGTLVPGLI
                     EWPARFPQPRSIKARASTSDLLPTICALVNQPPPKRPLDGIDLTPVLDGTATERPAPL
                     YFWEFNTQGLAAAKPTPWIDPALQEGTTPLVKLMAGKATRDFQNFRHPAITEDDYLGP
                     RAIIDGRFKLVIHETAGGGSRRELFDLTTDPAEKSNLLTQQAEVAGRLQSQLRQWQKS
                     VLHSLTGADYVE"
     gene            1231..1872
                     /locus_tag="VFV87_00880"
     CDS             1231..1872
                     /locus_tag="VFV87_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102333.1"
                     /translation="MIIFLNDESAYHYWVTHHRLGFVLDGRRKPQLNHFVLHRADCTV
                     LKGAKRHYWTTRGHLKACSLDRAELESWAAEECDDSVEHCPHCQPALDRPGDDSSDIH
                     LTKLGGEILEYVLEAALIHMEQEHPPYRLSIDDIAACFAKSPGQLVQTLQNLLDQGLL
                     TASGAGSRISSASGRRHVFPTAKSLRTLDAFRDASEADIAHELEKLHADQSGE"
     gene            complement(1892..3547)
                     /locus_tag="VFV87_00885"
     CDS             complement(1892..3547)
                     /locus_tag="VFV87_00885"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5102334.1"
                     /translation="MAGAIGLLTSCNLACFVSQGAESPRRPNIVIILADDMGYSDLGC
                     YGGEIETPNLDALAASGLRFTQFYNTARCWPTRGALLTGYYAQQIHRDGLPEVGGGGR
                     GVRQPWARLLPELLAPAGYRSYHSGKWHVDGPVLAGGFLRSLDMRNQGNFFTAAGNAI
                     DDVPVKVPADEKGYYATTAVVDHAIDCLKDHAANHADKPFFHYLAFIAPHFPLHAPPQ
                     DIAKYRDKYLGGWEELRQARFARQQKLGIVNTTLSELERDVGPPYDFPDALVKLGPGE
                     VNRPLPWTELTDEQRRFQATKMAIHAAMVDRMDQEIGRFIAQLKATGDFENTLILFAS
                     DNGASAEIMVRHGGHDPSAEPGSAATYLCLGPGFSSAANTPFRRHKTWVHEGGISTPL
                     IVHWPQGIAAKGELRRTPAHVIDIVPTVLELAGVEKPREHHGEPIPPAPGKSLVPALA
                     KDVAIPRDSLWWLHEGNRAVRVGDWKLVAANNEPWELYDLSTDRAEQHNLAAKMPAKA
                     EELAQVWQRQTDDFIELAKKTLDLQPQAGAKGKAKAKAKSAAK"
     gene            complement(3874..4350)
                     /locus_tag="VFV87_00890"
     CDS             complement(3874..4350)
                     /locus_tag="VFV87_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX5102335.1"
                     /translation="MRHLVLIAAAGLLAGCSATAASNASPEDVPGIREDVSGAGDIDN
                     WQGRWQLVSYTADGQTAQADIQWIVYADHYVIQGGDMTGQGSYPYVLDPSQKRIDVHH
                     HDTPAATYGGHFKGIYELQGDSLAVCYDLAGQRYPNSLDASPGSRQVLYQFQRQSR"
BASE COUNT          986 a         1856 c         1660 g          949 t
ORIGIN      
        1 tggtcgctgc gtccccagga aaccacgatc gcacacattc tgcgaagcgc cggttaccag
       61 tgcggccact tcggcaaatg gcacctcggt cccgtcaaag ccgattcgcc taccaacccc
      121 ggcgcgatgg gctttcacga atgggtctcg cacgacaact tcttcgagct gaacccttcg
      181 ctctcgcgca acggcggtcc gccggaagtg atcaagggag aaagctctga aattgttgtg
      241 aaagaagcca tccgtttcat cgacggcgcg aagcgtgccg gaaagccgtt cctggcggtc
      301 gtctggttcg gctcgccgca cgagccttac agcggcctgc ccgaagatct cgcgctctac
      361 gacgacttgc ccgcgaaata caccaggctg gtcggtctca catccaacga aaccggcggc
      421 cctacccggc ggccgcaagg cgaagtgctt cgcgagcgat atgccgagat taccgcgatg
      481 gaccgcgcca tcggccattt gaggaaacat ctcgccgaca cagggctgcg tgacgacacg
      541 ctgctgttct actgcggcga caacggcacc tcggccgatt ccgctctggg tgagccgcat
      601 cgcggcgtca aagggcaggt ctatgagggt ggaacgctgg tcccgggtct gatcgaatgg
      661 ccggcgcgat ttccccagcc gcggagcatc aaagcccgcg cgagcaccag cgatctcttg
      721 ccgacgatct gcgcgctggt caatcagccg ccgccaaagc gtccgctgga tggaatcgac
      781 ctcactccgg ttctcgacgg caccgccacc gagcggccag cgccactcta cttttgggag
      841 ttcaacaccc aagggctcgc ggctgcgaag cccacgccct ggatcgatcc ggcactacag
      901 gagggcacga cgccactggt gaagctgatg gccggcaagg cgacgcggga cttccagaac
      961 ttccggcatc ccgcgattac cgaggacgat tacctcggcc ctcgcgcaat catcgacggc
     1021 cgcttcaagc tcgtcattca cgagaccgct ggcggcggct cacggcgcga gctcttcgac
     1081 ctcacaaccg atccggcgga aaagtccaat ttgctgacgc aacaagccga agtagccggc
     1141 agactccaat cccagcttcg ccagtggcaa aagtcggtcc ttcacagtct cactggagcc
     1201 gactacgtcg agtaacggca cccgtggccg atgatcatct tcctgaacga cgaaagtgcc
     1261 tatcactact gggtgacaca ccatcgcctg ggcttcgtgc tcgacggccg ccgcaagccg
     1321 cagctcaacc atttcgtgtt gcaccgcgcg gattgcaccg tgctcaaggg tgccaagcgg
     1381 cactactgga cgacccgcgg ccatttgaaa gcctgctcgc tcgatcgggc ggaacttgaa
     1441 tcttgggcag ccgaagaatg cgacgattca gtcgagcatt gtccccactg ccagcccgcg
     1501 ctcgaccggc ctggcgatga ctcctcggac atccatctga caaagttggg cggagaaata
     1561 ctggaatacg tcctggaagc ggctctgatt cacatggagc aggagcatcc cccgtaccgc
     1621 ctctccatcg acgatatcgc agcctgcttt gccaaatcgc ccggccaact ggtccaaaca
     1681 ctacaaaacc tgttggacca gggactgctg accgcgagcg gcgcaggatc acgcatttca
     1741 tcggcatcgg gccggcgaca cgtctttccc acggccaagt ccctgcgcac gcttgacgcc
     1801 ttccgcgacg catcagaagc cgacatcgct cacgagcttg aaaagctcca cgcggaccag
     1861 agcggcgagt gaaacacccc tgggggcggc gtcacttcgc cgcactcttc gccttcgctt
     1921 tggccttccc cttcgctccg gcctgcggct gcaggtcgag cgtcttcttt gccagttcaa
     1981 tgaagtcgtc ggtctggcgc tgccagacct gggccagctc ttcggccttg gcgggcatct
     2041 tggccgcgag gttgtgctgc tcggctcggt ccgtgctcag gtcgtacagc tcccacggct
     2101 cgttgttggc ggccacgagt ttccaatcac cgacgcgcac cgcccggttc ccttcgtgca
     2161 gccaccacag gctatcgcgg ggaatggcca cgtccttggc cagcgcaggc acgaggcttt
     2221 ttcccggcgc gggagggatc ggctcgccat ggtgctcgcg cggtttctcc acgcctgcca
     2281 attccagcac ggtcggcaca atgtcgataa cgtgcgccgg cgtgcggcgc agctcccctt
     2341 tggcggcgat gccctgcggc caatggacga tgagcggcgt gctgatgcct ccctcgtgga
     2401 cccacgtctt gtgacggcgg aacggcgtgt tggccgcgct cgaaaagccc gggcccaggc
     2461 acagatacgt cgccgcgctg cccggctcgg cgctcgggtc gtggccacca tgccgcacca
     2521 tgatctcggc gctggcgccg ttgtcggagg cgaacagaat cagcgtattc tcgaagtccc
     2581 ccgtcgcttt aagctgcgcg atgaagcggc cgatttcctg gtccatgcgg tccaccatcg
     2641 ccgcgtgaat cgccatcttc gtggcctgaa accgccgctg ctcgtcggtc agctcagtcc
     2701 acggtagcgg gcgattcact tcgccgggtc cgagcttcac cagcgcgtcg gggaagtcat
     2761 acggcgggcc cacgtcgcgc tccagctccg agagcgtcgt atttacaatg cccagtttct
     2821 gctggcgggc aaagcgggcc tggcgcagct cctcccaccc cccgagatac ttgtcgcggt
     2881 acttcgcgat gtcttgcggc ggagcgtgca gaggaaagtg cggggcgatg aacgccaagt
     2941 aatgaaagaa cggcttgtcg gcatgattcg ccgcgtggtc tttcaggcag tcaatggcgt
     3001 gatcgacgac agcggtcgtt gcatagtagc ctttctcgtc cgcgggcact ttgaccggca
     3061 catcgtcgat cgcgttgccg gccgccgtga aaaaattccc ctggttccgc atgtcgagcg
     3121 agcggagaaa gccgccggcc agcaccgggc cgtccacgtg ccacttgccg ctgtgatagg
     3181 agcgatatcc ggccggcgcc agcagctccg gcaacaggcg cgcccacggc tggcgcacgc
     3241 cgcggccgcc gcccccgact tccggcagcc cgtcgcgatg gatctgctgg gcgtagtacc
     3301 cggtgagcag cgccccgcgc gtcggccagc agcgagccgt gttatagaac tgggtgaacc
     3361 gcagcccgct ggcagccagc gcgtccaggt tcggcgtctc gatctccccg ccgtagcagc
     3421 ccagatccga ataccccatg tcatcggcca agatgatcac gatgttcggc cgccttggcg
     3481 attctgcgcc ctgtgagaca aaacaggcca gattgcacga ggtcagcaat ccaatcgccc
     3541 ctgccatcaa agcaaagtgg tgtgacacat ttcgccaatc ctggcccttt cgcggacaac
     3601 cagaaagcag cgcgaacaac gtgatagtac acatggctag tcaatctccg gcccgcagca
     3661 gggaataaac gcgactaatt ctcgtcgact agacctcacc ggtcaaacat ccggaatgac
     3721 gccatgaccg cttggctccg cagccgaaca tgggtcgaga tcacttcgcc gtcgaagcaa
     3781 gaaaagcgct gccggtccta tgggtcggct tggcatcggc ggcaaaccga ctgccgcgac
     3841 ggcaaccttg ccgggaaatc cctggggcgg gcgctagcgg ctttgtcgct ggaattgata
     3901 gaggacctgc cgcgagccgg ggctggcgtc cagcgaattc ggatatcgct ggcccgcgag
     3961 gtcgtagcac accgcgagcg agtccccttg gagctcgtag attcccttga agtggcctcc
     4021 gtacgtcgcg gctggcgtgt cgtgatgatg cacgtcaatg cgtttctggc ttggatcgag
     4081 cacgtacgga tacgacccct gaccggtcat atccccgccc tggatcacgt agtggtcggc
     4141 gtaaacaatc cattggatgt cggcctgcgc cgtttggccg tccgccgtgt aggaaacgag
     4201 ttgccacctc ccctgccagt tgtcgatatc cccagcgcct gacacgtctt cgcggatgcc
     4261 tggcacgtct tccggcgagg cgttggaagc ggcagtcgca ctgcagccgg ccagcaagcc
     4321 ggccgcggca atcaaaacca ggtggcgcat ggtcagattc tcctgcgaat ggttgcgaag
     4381 gcatgtcccc aaggtgattt agctcgcacc agccagcgcg catcggcggc ttcaagtacc
     4441 gaaaccgccc gtgcgaagct gaagtcgggt tgcagcaacg gcgcgagttt tatggccagg
     4501 ttcgcctccc ggcggcctcc tcaatcgggt cgcccgcgcc aggtgtgccc caacggggca
     4561 acccgcgcca gcccaggcca acggcctggg aacgaggtcg aaaaatgcag attcaaaacg
     4621 tattcagggc ggcgctgcgc ttgccctggg ctaccacagg tcggcccgtt gggccgcaag
     4681 atcgcccatc ggccaagccc catcgcagcg cgcccctgat ccaaaagccc ttgttgtgcg
     4741 cgggtctccc gaccccgcac ttggcttgag caggtctcct cgctcggcgg agcagggaga
     4801 ccttcggtcg gccgtttcgg cgctcttcag gagacccgcg ccgaacacgg cccatcggcc
     4861 aagccccatc gcagcgcggc cctggtccaa aagcccccgt tgggcaacct ggcgagaagg
     4921 gtcacccctc ctccttcgct cggcacttgc caggacgcca gtagcccgcg gcgaggatag
     4981 tagcccgtag cgcaagcgag ggttaagcgg gtcgcccggg aaacagcgtg gtggtgcgac
     5041 cacggatgga ccgagtgcga cgcgggccgg tcgcggcggc ccgccgcgtg acgccgcccc
     5101 cattaccctc gcttgcgcta cgggctacga tggcgacccg ttggacgacc tggcgagaag
     5161 ggccacccct cttccttcgc tcggcactgg ccaggacgcc agtagccacc cctgggccag
     5221 cccagcccgt cagtcccacc ccgccgccgc gcccacgcgg cgatgcttgc cccaacgggg
     5281 caacctgcgc cagcccaggc ccacggcctg ggaacaaggt cgcaccatgt gcgttcaagc
     5341 cccaacgggg cgcgacggcc gggttgcgcg gcgcgcgtga cgattcgttg gcccccctgt
     5401 tcgtttcgcc ccttcagggc ttcatgcgcg ttgcggacat cgtacccagg g
//
LOCUS       DASUGB010000045         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_700808, whole genome shotgun sequence.
ACCESSION   DASUGB010000045 DASUGB010000000
VERSION     DASUGB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700808"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..638)
                     /locus_tag="VFV87_00895"
     CDS             complement(432..638)
                     /locus_tag="VFV87_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102336.1"
                     /translation="MLNSVLGVVRNGKIELSEAAPLEEGARLLVTFLPLADEQEFWLQ
                     ASQPSLAEIWDNPADDAYAQLLQT"
     gene            738..1523
                     /locus_tag="VFV87_00900"
     CDS             738..1523
                     /locus_tag="VFV87_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5102337.1"
                     /translation="MSAQYLQQLFGLDEQVAVVIGGTGVLGGALCDGLAQAGAQVVVA
                     GRSEERGHARVEAIQAIGGEATFLPVEADKRLSMQSLLDASITAYGQVDMLVNCAGTN
                     SAVPYEQITDEDWHKVVDSQLTATHLGCQIFAPFMTTQTHGGSILNIGSVTSHTPLSR
                     VFAYSAAKAAVVSLTKNLAREYAKRNVRVNVLCPGFFPAEQNRKILDKERVENIMRGT
                     PMQRFGEPHELVGAALLLLSRKAGSFMTGEDIYVDGGFTSMRF"
     gene            1542..2996
                     /gene="zwf"
                     /locus_tag="VFV87_00905"
     CDS             1542..2996
                     /gene="zwf"
                     /locus_tag="VFV87_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5102338.1"
                     /translation="MAHTVVIFGASGDLTSRKLIPALYSLHVKGRLPDQTRIVGVSRT
                     KFSSEAWREQLAETTAKFAGQDFQAESWKAFAAHVFYHPGDIDQPASFAELSKLLEKL
                     EGGASAMRLYYLATAPSLYAPAIGQLGAAGLADETQGVRRIVIEKPFGTDLASAEALN
                     KETHQVFREEQVYRIDHYLGKETVQNLLVLRFANSIFEPIWNRRYIDHVQITVAEEVD
                     VGRRAGYYDHAGVLRDMFQNHLLQLLMITAMEAPARYSSEFVRDEKVKVLGAIQPLTP
                     EAVGTETVRGQYEGYLQAEGVTPGSQTATFAAIKLNIDNWRWSGVPFYLRSGKAMSCR
                     TSQIVIQFRQPPIMMFGGGRCAEHEPNRLVIQVQPAEGIQLHFQTKVPDGGMRLTTQD
                     LAFEFAQEFQGAMPDSYQRLLLDAMNGDASLFARSDEVEVAWGIIDPVIAAWQTPAAP
                     KLETYPRGDWGPDASNTWMQAQRREWFDVCPVLH"
BASE COUNT          596 a          938 c          933 g          557 t
ORIGIN      
        1 aactcaaaca ccaactggcc cacagactgt atattcacaa ccagcctcct tgcgctttgg
       61 gaagtcgttg ttactcaact tcttagacgc gtctggaggc tgtttcattt catgggccgt
      121 gaataatccg ggctagccag acgcggagcg attggtccag ggcctgccca tcgcgcgccg
      181 acaattggcc gatccgcttc acgacgagcc gcgggtgaat cgtgaaaatg cctcgtttcg
      241 cggccgttgg gatgtgcagg cctgcagcct tccaatcggc aagcacgaac tcgccagcgg
      301 caagtccggc tgtttggctc gtgagcggga caacgaagtg atcaaccgat gcgtgaggcg
      361 cttaaacgac aacagctgga tgcaccttcg tcgcggtaag atcggtgaag ggataatgca
      421 ccagcacgac gtcatgtctg gagaagctgg gcatacgcat cgtccgctgg gttgtcccaa
      481 atctctgcga gcgacggctg gctggcctgg agccagaatt cttgctcgtc ggcaagagga
      541 aggaatgtca cgagcaatct ggccccttcc tcgagcggcg cagcttccga cagctcgatc
      601 tttccgtttc gcacgacgcc caggacggaa ttgagcatag ctgactccaa attgcttggg
      661 gcggttcacc cgtagccgcc agcctgtcat attatactcc gcaatccaga tcagccgatc
      721 tcgaatcccg gaattccatg tccgctcaat acttgcagca gctctttggc ctcgatgagc
      781 aagtcgccgt cgtcattggc ggcaccggcg tcttgggcgg cgcgctgtgc gacggcctcg
      841 cgcaagctgg agcgcaggtg gtcgtcgccg gccgcagcga ggagcgcggg cacgcccgcg
      901 tggaggccat tcaagcgatc ggcggcgaag cgacctttct gcccgtcgaa gccgacaagc
      961 ggctgtcgat gcaatcgctg ctggacgctt cgatcacggc ctatgggcag gtcgatatgc
     1021 tcgtcaactg tgccgggact aattcggcag tgccctacga gcagatcacc gacgaagact
     1081 ggcacaaggt cgtcgatagc cagctcacgg cgacgcacct gggctgccag atcttcgccc
     1141 ccttcatgac cacgcagacg catggcggct cgatcctcaa tatcggcagc gtcacgtcgc
     1201 acacgccgct ttcgcgggtt ttcgcctatt cggcggccaa ggcggcggtt gtgagcctga
     1261 ccaagaacct ggcccgcgaa tatgccaagc ggaacgtccg cgtgaacgtg ctctgccccg
     1321 gcttcttccc tgccgaacag aaccgcaaga ttctggacaa ggagcgcgtc gaaaacatca
     1381 tgcgcggcac tcccatgcag cgctttggcg agccgcacga gttggttggc gcggccctcc
     1441 tcctgctctc ccgaaaggcc ggctcattca tgaccggcga agacatctac gtggacggcg
     1501 gatttacctc gatgcggttc tagccgctgc gcggaaccac catggctcac accgttgtca
     1561 tcttcggcgc ctcgggcgat ctcaccagcc gcaagctgat cccggcgctc tattcgctgc
     1621 acgtcaaagg ccgactgccc gaccagacgc ggatcgtggg cgtttcgcgg acgaagttca
     1681 gctccgaagc ctggcgcgag caactcgcgg agaccacggc gaaattcgct ggtcaggatt
     1741 tccaagcaga atcatggaaa gccttcgccg cccatgtgtt ctatcacccc ggcgatatcg
     1801 atcagccggc gagctttgcc gaactgtcga agctgctgga gaagctggaa ggcggggcga
     1861 gcgccatgcg gctgtactac ctggcgacgg cgccgagctt atatgcgccg gcgatcgggc
     1921 agcttggcgc ggcgggactt gccgatgaaa cgcagggcgt gcggcggatc gtgatcgaaa
     1981 agccgttcgg gaccgatctg gcatcggccg aagcgctgaa caaggaaacg caccaggtat
     2041 tccgcgaaga gcaggtctat cgcatcgacc attatcttgg caaggagacg gtgcaaaacc
     2101 tcttggtgct gcgattcgcc aattcgattt ttgaaccgat ctggaaccgg cgctatatcg
     2161 accacgtgca gatcacggtc gccgaagagg tcgatgtggg ccgccgggcg ggctattacg
     2221 accatgccgg cgtactccgc gacatgttcc agaaccacct gctgcagttg ctcatgatta
     2281 cggcgatgga agcgccggct cgttattcgt ccgaattcgt ccgcgacgaa aaggtcaaag
     2341 tgctgggggc cattcagccg cttacgcctg aggcggtggg cacggaaacc gttcgcgggc
     2401 agtacgaggg ctacctgcag gccgagggtg ttacgccagg cagccagacg gcgacgttcg
     2461 cggcgatcaa actcaatatc gacaactggc ggtggagcgg cgtgccgttc tacctccgct
     2521 ccggcaaggc gatgagctgc cgcacgtcgc agatcgtcat tcaattccgc cagccgccga
     2581 tcatgatgtt cggcggcggc cgctgtgccg agcacgagcc caaccgcctc gtcatccaag
     2641 tccagcccgc ggagggcatt cagcttcact ttcagacaaa ggttcccgac ggcggcatgc
     2701 ggctgacgac gcaggacctg gcgttcgaat tcgcccagga atttcagggg gcgatgcccg
     2761 actcatacca gcgattgctg ctggacgcga tgaacggcga cgcgagcctg ttcgcccgca
     2821 gcgacgaagt cgaggtcgct tggggcatca tcgatcccgt aatcgccgcc tggcaaacgc
     2881 ccgccgcgcc gaagctcgaa acctaccccc gcggcgactg gggccccgac gcgagcaaca
     2941 cctggatgca ggctcagcgg cgcgagtggt ttgatgtgtg cccggtgctg cattgaatga
     3001 cacgtagggt gggacaaggc cgcc
//
LOCUS       DASUGB010000046         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_561922, whole genome shotgun sequence.
ACCESSION   DASUGB010000046 DASUGB010000000
VERSION     DASUGB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561922"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="VFV87_00910"
     CDS             complement(<1..1998)
                     /locus_tag="VFV87_00910"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEX5102339.1"
                     /translation="MAEYSWPAKDKATTIGQRVKRLDGPEKSTGKAKYTYDVNLKNML
                     VAKGLGCPHGHCKIKVIDVAPALKVPGVVHAEALKQSGADVEFQGDLLAVVAAESEGA
                     AREGIAAIKLEYDPLDVFVTEEDLKAAEAAGRTSKGGGKTQTEKEPGDNDDEDAFVAK
                     EIDRLLKESAHTVEGYYGIDVITHCCLEPHGCTVMWEGNKLIAYLSTQNVSGTDEGFA
                     NDLKIASSDVEVKCDYIGGGFGSKFAPDYWSTTAAKIAKATGRPVKFMLDRDQELKIA
                     GNRPSGFIKVRVGADKDGVITVWDSEHWGTTGVTPGGVSQSVIPYVLAPKNYRRNQIN
                     IKTNNGQARAWRAPNHPQACAMTQTALDDIAAKMQADSLDVFMRNLGTDAAPLVAGAK
                     PSVYKAEMEIGAKLMDWKAKWHPHGKGPAKGSVVDGLGLAFHTWGGGANQSTCTLRVY
                     PNGDVETFCGTQDLGTGTRTVIALVLAETFGLPYDKIRVNIGSSKMPVSGPSGGSTTV
                     GGVSESHRRAGQDAFAKLADLVGKKLEVDPATLVAAGGRVHVAGTPDKGLSWKEACTL
                     IGMQPLEVQGNFVRGTPSPLSSSQVGGVQMAHVEVDKETGVVKMKKFVAVQDMGLIIS
                     PQQAESQINGAVIMGIAAALFEQRINDPKTGAFVNAELADYK"
     gene            complement(1991..2602)
                     /locus_tag="VFV87_00915"
     CDS             complement(1991..2602)
                     /locus_tag="VFV87_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5102340.1"
                     /translation="MSERTSTLKPPSGFSRRDFLRGSGAVAAGAALQQTATAVAQEAK
                     GVPGATGATTIKLDVNGKTHEVKVEPRTTLLEALRYQLDLTGAKPICTDGSSGGATVL
                     IDGKPMQANMMLALQAAGKKIQTVESLEGDAVPQAFVAADAQQCGFCTPGFVVAVKAF
                     LTKNPKATDEEIRKGLNGNLCRCGTYANIIQACTMVVKGGSNG"
     gene            2812..>3414
                     /locus_tag="VFV87_00920"
     CDS             2812..>3414
                     /locus_tag="VFV87_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5102341.1"
                     /translation="MLPFDAQSADGLLIAAALAGEREAFAHLVRRYQGSLVKAAISRL
                     GRRTWAEEAVQETFLCAHRWLATYDSRFSFRTWLWTILLNQCSRVAKRESKHVVQTAA
                     ETLPHERIDPRPSPLKLLLAKEASEQLYELLARLPDVQADALRLRFFGGLTFPEIAAA
                     MKCSEPGAKNRVKSGLTTLSKWLVTGETPAPREMAGQRPAP"
BASE COUNT          514 a         1066 c         1147 g          687 t
ORIGIN      
        1 cttgtaatcg gccagctcgg cgttgacgaa cgcgccggtc ttcgggtcgt tgatccgctg
       61 ctcaaacaga gccgcggcaa tccccatgat gaccgcgccg ttgatctggc tctcggcctg
      121 ctgcgggctg atgatcaggc ccatatcctg cacggcgacg aacttcttca tcttgacgac
      181 gccggtctcc ttatcgacct ccacgtgcgc catctgcacg cccccgacct ggctgctgga
      241 aagcgggctg ggagtgccgc ggacgaagtt cccctgcacc tccagcggct gcatgccgat
      301 gagcgtacag gcttccttcc acgacaggcc cttatcgggc gttcccgcga cgtgaacgcg
      361 gccacccgcg gccacgagcg tcgccggatc gacctccagc ttcttgccga ccaggtcggc
      421 cagcttggcg aaggcgtcct gcccggcccg gcggtgcgat tcggagacgc cgccgacggt
      481 cgtgctaccg cccgatggtc cgctgaccgg catcttggaa ctgccgatat tgacgcggat
      541 cttgtcgtac gggaggccaa acgtctcggc cagcaccagg gcgatcacgg tccgcgtgcc
      601 ggtccccagg tcctgcgtgc cgcagaaggt ctcgacatcg ccattgggat agacacggag
      661 cgtgcaagtt gactggttcg caccgccgcc ccaggtgtgg aacgccaggc ccaggccatc
      721 gacgaccgaa cccttggcgg ggcctttgcc gtgcggatgc cacttggcct tccaatccat
      781 cagcttggcg ccgatttcca tctcggcctt ataaaccgag ggcttggcgc cggcgacgag
      841 cggagcggcg tcggtcccca ggttcctcat gaagacgtcg aggctgtcgg cctgcatctt
      901 ggcggcgatg tcgtccagcg cggtctgcgt catggcgcag gcctgcggat gattcggggc
      961 ccgccaggcc cgcgcttgac cgttgttggt cttgatgttg atctggttcc gccgatagtt
     1021 cttcggggcc agaacatagg gaatgaccga ctggctgacg ccgccgggcg tgacgcccgt
     1081 ggtcccccag tgttccgaat cccagacggt aatgacgccg tccttgtcgg cgcccacgcg
     1141 aaccttgata aagccgctgg ggcggttgcc ggcgatcttc agctcctggt cgcggtcgag
     1201 catgaacttg accggtcggc cggtggcctt ggcgatcttg gcggctgtgg tgctccagta
     1261 atcgggggcg aacttgctgc caaacccgcc gccgatatag tcgcacttca cctcgacgtc
     1321 gctggaggcg atcttcaggt cgttggcgaa gccctcgtcg gtgccgctga cgttctgcgt
     1381 cgagagataa gcgatcagct tgttgccctc ccacatgacc gtgcagccgt gcggctccag
     1441 gcagcaatgg gtgatgacgt cgatgccgta gtagccctcg accgtatggg ccgattcctt
     1501 cagcagccga tcgatttcct tggcgacgaa cgcgtcttcg tcgtcgttat cgccgggctc
     1561 cttttcggtc tgggttttgc cgccgccctt gctggtgcgg ccggccgctt cggcggcttt
     1621 gaggtcttcc tcggtgacga agacgtcgag cgggtcatac tccagcttga tcgcggcgat
     1681 gccttcgcgg gccgcgcctt cgctctcggc ggcgacgacc gccagcaaat cgccctggaa
     1741 ctcgacatct gccccgctct gcttgagggc ttcggcatgg accacgccgg gcactttcag
     1801 ggccggggcc acgtcgatca ccttgatttt gcagtggccg tgcgggcagc cgagcccctt
     1861 cgccacgagc atgttcttga ggttgacgtc gtaggtgtac ttggccttgc cggtcgattt
     1921 ttccgggcca tcgagccgct tgacgcgctg gccgatggtc gtggccttgt ctttcgcggg
     1981 ccaggaatac tcagccattg gatcctcctt tcaccaccat cgtgcaggct tgaatgatgt
     2041 tcgcgtaggt cccgcagcgg cagaggttgc cgttgagccc cttgcggatt tcctcgtcgg
     2101 tggccttggg attcttggtg aggaacgcct taaccgcgac gacgaagccc ggcgtgcaga
     2161 agccgcactg ctgggcgtcg gcggcgacga aggcctgggg aaccgcgtcg ccttcgaggc
     2221 tttcgaccgt ctggatcttt ttgccggcag cctgcagggc gagcatcatg ttggcctgca
     2281 tcggcttgcc gtcgatcaac acggtcgctc cgccgctgga gccgtcggtg cagatcggct
     2341 tggcgccggt caggtcgagc tggtagcgga gggcttccag gagcgtggtg cggggctcga
     2401 ccttcacttc gtgggtcttg ccgttgacgt ccagcttgat ggtcgttgcg ccggtcgcgc
     2461 cgggaacgcc cttggcttcc tgggcgaccg ccgtggcggt ttgctgcaag gccgcgccgg
     2521 cggccacggc gcccgatccc cgcaggaaat cgcggcgact gaagccggag gggggcttca
     2581 gcgtactcgt gcgttctgac atgggtgatg aatccttgta gagtggcggt tgaaaacagc
     2641 cagccgcttg cggcaacctc cccgaccgac aacgaagcgc tggtctccgc tgtcccccgg
     2701 cgagggcgcc gtcaagcgcc ccagattatt gcggctggac aggagcgatg caatcaaggg
     2761 gagggcactt tcccgtttcg cggctggggg gtgtcgagta ggataagttg catgctgccg
     2821 tttgatgcac agtcggctga cggcctgttg atcgccgctg ctttggcggg cgagcgggag
     2881 gcgtttgcgc acctggtacg gcggtatcag gggtcgctcg tgaaggcggc gatcagccgg
     2941 ctgggaaggc ggacgtgggc ggaagaggcg gtgcaggaga cgtttctttg tgcccaccgc
     3001 tggctggcga cgtacgactc gcgattcagc ttccgcacgt ggctgtggac gatcctgctc
     3061 aaccagtgtt cgcgcgtggc gaagcgcgag tcgaagcacg tcgtacaaac ggcggcggaa
     3121 acgttgccgc acgagcggat cgatccgcgc ccttcgccgc tgaagctgct tctggcgaaa
     3181 gaggcgtcgg agcaattata tgagctgctc gcacgactgc cggacgttca ggcggatgcc
     3241 ttgcggctgc ggttttttgg cggtttgacg ttcccggaaa tcgccgcggc gatgaaatgc
     3301 agcgagccgg gggcgaagaa tcgtgtgaag tcgggattga cgactttgtc gaagtggctc
     3361 gtgacaggcg agacgcctgc cccacgagaa atggcaggcc agaggcctgc ccca
//
LOCUS       DASUGB010000047         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1673523, whole genome shotgun sequence.
ACCESSION   DASUGB010000047 DASUGB010000000
VERSION     DASUGB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673523"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..>2522
                     /locus_tag="VFV87_00925"
     CDS             417..>2522
                     /locus_tag="VFV87_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5102342.1"
                     /translation="MDFERLFAPFRPPGTDFADRLRYWAAEIPDDTAFAFWTGDEQDA
                     ARLSYAELDTQARAIAAELTARRLRGQRALLLFPPGLEFVSALFGCFYAGVVAVPAYP
                     PRRSRNMERIDAISEDAQAAVVLSTQAITDRPPVEQAPHLKRIPWIATDRLPDDLATQ
                     WEPPRLAADALAILQYTSGSTGLPKGVMLTQSNLMHNCGLITHGFEPSRNTVGLFWLP
                     TYHDMGLIGSLLEPLFLGRPSVLMPPLAFLQKPVRWLRGISRFRATISGGPNFAYALC
                     NEKITPDECVGLDLSSWEVAFNGAEPIRAETLEAFSEKFAPYGFRGHSHYPCYGMAET
                     TLLVTGGTKRARPVIRNFEGAALDDHRVVAASDEHQPSRKLVSSGRAMPGDEIVIVDP
                     DSSRPLPAGSVGEICVAGASVAQGYWNKPEETARVFGLQLEGHTRTFLRTGDLGFLHE
                     GELFVTGRCKDLIIIRGRNLYPHDVELTVEKCHPALRTAGGACFSVEVAGEERLIVVQ
                     ELERQQAQADGQAIIAAIREAVMIEHEAPLFSVCLLKAGKLPKTSSGKVQRRACREAY
                     LQGGLESQAQWTDEVIPAPRAAGSAETAPAPAGAGSRSAAAIQSWLAEGIAKRLRRNP
                     AEIDVHEPFARFGLDSLALVELSGELQQYLGREVSPTVLYSYPTISELARHLAAPHVA
                     ALEQAGGQESSAEPIAVVGL"
BASE COUNT          411 a          804 c          832 g          475 t
ORIGIN      
        1 aggaatacct cgcttacgct tcgggttggt gtggagtccc tcgcttacgc ttcgggttgg
       61 tgtggagtcc ctcgcttacg cttcgggttg gtgtgcgggt tgcgaaaatc tacgcagttc
      121 atacagattg tgcagattac cgcattcggc tcattttaaa tgaggcggag cattggcagc
      181 ccgctgacaa ctcgttcatt ctctggtagg ttcgaccgct cgacaatctg ctgcatttgg
      241 ctgcccgcga gatgggagac tggtctccga tcgccgcagc gacgatacct gccagcggcc
      301 gcggcgatgc tccctgcctg tgccgcaagc cgtgctccca gcaagcggat tggtcggacg
      361 caacatagtg ccacggaagg atcacaggat gtcctcttgc gcgagcgata ttgacggtgg
      421 atttcgaacg tctattcgca cccttccggc ctcccggcac cgatttcgcc gatcgtctcc
      481 gctattgggc ggccgaaatc ccggacgata cggctttcgc cttctggacc ggcgacgagc
      541 aggacgcggc gcggcttagc tatgccgagc tcgataccca ggcgcgggcc atcgccgccg
      601 agctgaccgc ccgccggctg cgcgggcagc gggccctctt gctgttcccg ccggggctgg
      661 aatttgtctc ggccctgttc ggctgctttt acgccggcgt tgtcgcggtt cccgcctatc
      721 cgccgcgccg cagccgcaac atggagcgga tcgacgcgat cagcgaagac gcccaggcag
      781 cggtggtcct ttcgacgcag gccattaccg accggccgcc ggtggagcaa gctccgcacc
      841 tcaagcggat tccgtggatc gccaccgatc gcctgccgga cgacctggcg acgcaatggg
      901 aaccgccgcg attggcggcg gatgccctgg cgatcctgca atacacctcg ggctcgaccg
      961 gcctgcccaa gggggtgatg ctgacgcagt cgaatctgat gcacaactgc gggctgatca
     1021 cgcacgggtt cgagcccagc cgcaacaccg tgggcctgtt ttggctgccg acgtatcacg
     1081 acatgggtct gatcggcagc ttgctggagc cgctgtttct ggggcggccg agcgtgctga
     1141 tgccgccgct ggcgtttttg cagaagccgg tgcggtggct gcggggcatt tcgcggtttc
     1201 gcgcgaccat cagcggcggt ccgaacttcg cctacgccct gtgcaacgag aagatcacgc
     1261 ccgatgagtg cgttgggctc gatctgagca gttgggaggt cgccttcaac ggggccgaac
     1321 cgatccgggc cgaaacgctc gaggccttca gcgagaagtt cgctccctat ggctttcggg
     1381 ggcattcgca ctatccgtgc tacggcatgg cggaaacgac gctgctggtc actggcggaa
     1441 cgaagcgcgc ccggccggtc attcgcaact tcgaaggcgc ggccttggac gatcatcgcg
     1501 ttgtcgctgc tagcgacgag catcaacctt cgcgcaagct ggtcagctcc ggccgcgcga
     1561 tgccgggcga tgagatcgtg atcgttgatc ccgattcatc gcgccccttg cccgcaggca
     1621 gcgtgggcga aatctgcgtc gccggcgcca gtgtcgccca gggctactgg aataagccgg
     1681 aagaaacggc ccgcgttttc ggcctgcaat tggaagggca caccagaacg ttcctccgca
     1741 cgggcgacct gggctttttg cacgagggtg agctgtttgt caccggccgc tgcaaggacc
     1801 tgatcatcat tcgcgggcgg aatctttatc cccacgatgt cgagttgacc gtcgaaaaat
     1861 gccatccggc tctgcgcact gccggcggcg cttgcttctc ggtcgaagtg gcgggcgaag
     1921 aacgactgat cgtggtccag gagctggagc gtcagcaggc ccaggccgac gggcaggcga
     1981 tcatcgccgc cattcgcgaa gcggtgatga tcgagcacga agcgcccctc ttcagtgttt
     2041 gcctgctgaa ggcgggcaag ctccccaaaa cctcgagcgg caaagtgcag cggcgggcct
     2101 gccgcgaggc gtatctgcaa ggcgggctgg aatcgcaggc gcagtggacc gacgaggtga
     2161 ttcccgcgcc ccgcgctgcg ggttccgccg aaacagctcc cgcgcccgcg ggcgccggca
     2221 gccgttcggc ggcggcgatt cagtcgtggc tcgccgaggg gattgcgaag cgtcttcgcc
     2281 gcaatccggc cgaaatcgat gtccacgagc cgttcgcccg cttcggactc gattcgctgg
     2341 cgctggtgga gctgtcgggc gagttgcagc agtacctcgg ccgcgaagtt tcgccaacgg
     2401 tgctctacag ctaccccacg atttcagagt tggcccggca tctggccgct ccacacgtcg
     2461 ccgccctgga gcaagccggc ggccaggaat cgtctgccga gccaatcgcg gtggtggggc
     2521 tg
//
LOCUS       DASUGB010000048         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1395751, whole genome shotgun sequence.
ACCESSION   DASUGB010000048 DASUGB010000000
VERSION     DASUGB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395751"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="VFV87_00930"
     CDS             <1..1610
                     /locus_tag="VFV87_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5102343.1"
                     /translation="LRSRDAGDPAATREAAVLGGRRLPGSATVQGTAGYAGRNIASES
                     PALAQGFTIWGRTGLTGSRLGFGTYRVATEEAAHRDALIKALRAGCNVIDTSTNYMDG
                     DSERLIGSVLAELVKSGDLTREEVIVVSKIGYVQGNNLVQAQAREKTGQPYPDMVKYG
                     EGIWHCVHPDFLADQLAASLDRLGLDTLDVCLLHNPEYFLSQTKLNGKDQLASRRDEF
                     YRRLRQAFGYLESQVAAGRLRWYGVSSNTVIAAADDPEATSLSRMVDAAKEAAGTSRL
                     HHFAVLQCPMNLYESGAALTANTGSTGTTTPLALAQHEGVAVLVNRPLNAMSGRGGGM
                     MRLADVSVDEPAVAFDDQRARVAKLEEQYRREIAPSVQHSGQGMLPGDFFNWAEDLAK
                     IRGQVQGLEHWEQIEHQMIAPHVNQVLRALSQTLTGETAERWEAWRDAYVPELLRLLK
                     EVRREASERSRARSAAVGQRLDPLLPAERRGESLSRKALWVLVSTPGVTCVLNGMRST
                     PYVKDSLQVLRWEPLNEPLAVFRAFAS"
     gene            1674..>2049
                     /locus_tag="VFV87_00935"
     CDS             1674..>2049
                     /locus_tag="VFV87_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102344.1"
                     /translation="MTHWIRFCNQGVLMVAALAVVAGCAGRSGTSGGVTDEPKPLAVE
                     QVAPAPVAEVPPPPPDNEPVPLRSVEMAERNSTGTQNIPFPDVLFDFDQYVLRDDALT
                     TVEENAKRLKANGVKKILLEGRT"
BASE COUNT          398 a          628 c          652 g          371 t
ORIGIN      
        1 ccttgcgatc ccgcgatgcc ggcgatccgg cggccacacg ggaagcggcc gttctcggcg
       61 ggcggcgtct gccgggatcc gccacggttc aaggcacagc cggctatgcg ggacggaaca
      121 ttgcgtccga gtcacccgca ctggcgcagg ggttcacgat atggggcagg actggcttga
      181 ccggcagccg gctcggtttc gggacctacc gggttgctac ggaagaagcc gcgcatcgcg
      241 acgccttgat caaggccttg cgcgcgggct gcaacgtcat cgacacctcc acgaattata
      301 tggacggcga cagcgaacgt ctgatcggat cggtgctggc cgaactcgtc aaatccggtg
      361 atctcacacg cgaggaagtg atcgtcgtct cgaagatcgg ttatgtgcaa gggaacaatc
      421 tcgtccaggc gcaggcgcgc gagaagaccg gacagcctta tcccgacatg gtcaaatacg
      481 gcgagggcat ctggcactgc gtccatcccg acttcctcgc ggaccagctc gcggcaagcc
      541 tcgaccgctt ggggttggac acgctcgatg tctgtctgct tcacaacccc gagtattttc
      601 tctcgcagac aaagttaaac ggcaaggacc agctcgcgtc ccggcgcgat gagttttatc
      661 gacggctgcg acaagcattt ggctatctgg aatcgcaggt tgccgcgggc cgcctccgct
      721 ggtatggcgt gtcgtccaac acggtcattg cagcagccga cgatccggag gccacttcac
      781 tttcacgcat ggtggacgcg gcgaaggaag cagcagggac cagcaggctc caccattttg
      841 ccgtgctgca atgtcccatg aatctttatg aatccggggc cgccttgacg gcgaataccg
      901 gaagcaccgg cacgacaacg ccactcgccc tggcacagca cgaaggcgtg gccgtcctgg
      961 tgaaccgtcc actcaatgcc atgtccggcc gcggcggcgg catgatgcga ttggccgacg
     1021 tgtcggtgga cgagccggcc gtggcgttcg atgatcagcg ggcgcgcgtc gcgaaactgg
     1081 aagagcaata ccggcgtgag atcgccccgt cagtccaaca tagcggacag ggcatgttgc
     1141 cgggggactt ctttaactgg gccgaagact tggcgaagat acgcgggcaa gttcagggtc
     1201 tcgagcattg ggagcaaatc gagcatcaaa tgattgcccc gcacgtcaac caggtgctgc
     1261 gggcgctgag ccaaacactg accggagaga ccgccgaacg ctgggaagcg tggcgcgatg
     1321 cctatgttcc tgaattgcta cgcctcttga aggaagtgcg tcgcgaggca tcggagcgca
     1381 gcagagccag aagcgcggcc gttggacagc gtctggatcc cttgctgccg gcggagcgcc
     1441 gaggcgaatc gttgtcgcgc aaagcgctgt gggttcttgt gagcaccccc ggagtgacct
     1501 gcgtgctgaa cggcatgcgg tctactccct acgtcaaaga ctcgctgcag gtcctgcgct
     1561 gggagccgct gaacgaaccg ctggctgttt ttcgggcctt cgcctcgtaa atagtcttgc
     1621 ctcatccggt ataattgatt attctctccc gcaatctaca catggaggtg tccgtgaccc
     1681 actggatccg attctgtaac cagggcgttc tcatggtagc ggcgcttgcc gtcgtcgccg
     1741 gttgcgcggg ccgaagcggc acgagcggcg gagtcaccga cgaaccgaag ccgctggcgg
     1801 tcgaacaggt ggcgcccgcg ccggttgcgg aagtcccgcc cccgcccccc gacaacgaac
     1861 cggtcccgtt gcggtcggtt gaaatggcgg agcgcaattc caccggcacc cagaatattc
     1921 ccttcccgga cgtcctcttc gattttgatc aatacgtgct gcgtgacgat gccctcacca
     1981 ccgtggagga aaacgccaag cgcctgaagg ccaacggcgt gaagaagatt ctccttgaag
     2041 gccggacgg
//
LOCUS       DASUGB010000049         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_6637,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000049 DASUGB010000000
VERSION     DASUGB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6637"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFV87_00940"
     CDS             <1..284
                     /locus_tag="VFV87_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102345.1"
                     /translation="WCFDAEPKQLWKVPLADGSLAGIPLEATGSLILATRSGMLVRLA
                     ADSGKEQARIDLSQPLAAMLAGDGEQVFVATADGCLLKAALPKESATEP"
     gene            281..2569
                     /locus_tag="VFV87_00945"
     CDS             281..2569
                     /locus_tag="VFV87_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5102346.1"
                     /translation="MKHRLFHCVGVLFACVVGAGGTFAADGLYLGTPYDEVTLDENNG
                     NVLLKVKPLNLPGRRVPAAGARGTDLEVELLDRPGEKYKIEWAAITNVTLFEQMVLAE
                     AEEHVKAGRYDEAYPAFHFLETNHPQTPGLKEAIENFLWVQIGGAYKAAKHDEALALL
                     VELYERNPQRPGMNVAYERVTGELVKKHLADENYLAVRGLLRNLVERFPAARATVAAP
                     YETQLQTKAAALLTEAKALLAEGKRREAHQTCSQMLEVWPAVAGGQELALSIHQQHPL
                     VVVGVMSPLAGDPLENPGAWAAQRAGRLLGRPLIERTAAAGEHGPYRSALVQFAPGED
                     AAQLQLKVLPNLRGLSGSRDVSAHDVSRCLLAQADPALAVFDPSWADVFASVTVQSGT
                     DLLVSFRQPQLHAAAWLSSPLFAGGGAAGQAAEVVRLGPYALNVQTPQQVSFIRQPDY
                     SLAGPSQPAEVVERTYRDSAVALRALRRGEISVIDRISPWELTRLASSGDIKIEPYAL
                     PRVHVLLPNSHRSLTANRTFRRAIAYAIDNVETLNRGLLDGQKVAGCEALSGPFPRGP
                     AYNEEVAIRPFDPGLAMTLARMTADEVNAARQDRGEPPLEMPPKLVLAHPAEPIARIA
                     CQSIARQLRLAGLMVTLREQEPGQAAGEDVDLLYADFAMHEPVVDAWRLLGPGGAVGD
                     CSPAMLALLRSLQAATDEQQALAALHEIHRLAAAELPAIPLWQLTDHFAWHTSVQGIG
                     QRPVTLYENIEQWQVEFRIPQQ"
     gene            2603..>3597
                     /locus_tag="VFV87_00950"
     CDS             2603..>3597
                     /locus_tag="VFV87_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102347.1"
                     /translation="MTRIVRIRHTVQAALMTLLVTTSADASGAEPWELTPYRIKLLVA
                     VEAAGGLPRDIESVLAQDLVARGAAVVGGAWRVDASAAPAELRHKLIHAIDTVSANEI
                     AGSAGEYDKVILVGALQESGKVLIQARELDLVTELWNVVVRRDAPQAELVPQTAFAAV
                     LQAFAPLARIESANGETATLRLRAGGLARRDRRLPALAPGVAFRPVLVKSDASGTLTR
                     GSAEALANVYLSPTNTRGAVVTCRVDAGAAAPLIPEYHPHRQRLALAVSPSSESTQLR
                     LVSIGDPPAPLEGLDVLDRGELLGRSDRRGLVTIAPDAEAVRALTIRRGERTLATL"
BASE COUNT          585 a         1185 c         1238 g          589 t
ORIGIN      
        1 tgtggtgctt cgacgccgag cccaagcagc tttggaaagt gccgctcgcc gacggctccc
       61 ttgcgggcat tcccctggaa gcgacaggca gcctgatcct ggcaactcgc agcggaatgc
      121 tcgtgcgact ggccgccgac agcggaaaag agcaggcccg catcgatcta agccagccgt
      181 tggccgcgat gctggcgggc gatggcgagc aggtgtttgt ggcgacggcc gacgggtgcc
      241 tgctgaaggc ggccttgccg aaggagagcg cgacggaacc atgaagcacc gcctttttca
      301 ttgcgtcggt gttctattcg cttgcgttgt cggcgcgggg ggaacgttcg ccgccgacgg
      361 cttgtatttg ggcacgccgt acgacgaggt cactctcgac gagaacaacg gcaacgtgct
      421 gctcaaagta aagccgctga acttgccggg ccgccgcgtt ccggcggcgg gggctcgcgg
      481 gaccgatctg gaagtcgagc tgctcgaccg gccgggcgag aagtacaaga ttgaatgggc
      541 tgccatcacc aacgtcacgc tcttcgagca gatggtcctg gccgaggcgg aggagcacgt
      601 caaggcgggc cgctatgacg aggcctatcc cgcgttccac tttttggaga cgaatcatcc
      661 gcagacgccc ggcctcaaag aagcaatcga gaattttctg tgggtgcaga tcggcggggc
      721 gtacaaagcc gcgaagcacg atgaggcgct ggccctgctg gtcgagctgt acgagcgcaa
      781 cccgcagcgg ccggggatga atgtcgccta cgagcgggtc acgggcgagc tggtgaaaaa
      841 acacctggcg gacgaaaact atctcgccgt gcgcggcctg ttgcggaatc tcgtcgagcg
      901 gtttcccgcc gcccgggcca cagtcgccgc tccctacgaa acgcagcttc aaacgaaagc
      961 cgccgcgctc ctgaccgaag cgaaggcgct gctggcggag ggcaagcgac gcgaggccca
     1021 tcagacgtgc agccagatgc tcgaagtctg gcccgccgtt gcgggcggac aggaactggc
     1081 tctctcgatc catcagcagc acccgctggt ggtcgtgggc gtcatgtcgc cgctggccgg
     1141 cgatccgctg gagaatccgg gcgcctgggc tgcccagcgg gccgggcgct tgctcggccg
     1201 gccgctcatc gagcgcaccg ccgccgcggg cgagcacggg ccgtaccgtt ccgccctcgt
     1261 ccagtttgct ccgggcgaag atgcggccca attgcagctc aaagtgctac cgaatctccg
     1321 cggcttgtcc ggcagccgcg atgtttcggc ccacgatgtg tcccgctgcc tcctcgcgca
     1381 agccgatccg gccctggcgg tctttgatcc ctcctgggcc gatgtgttcg ccagcgtcac
     1441 ggtccagagc gggaccgacc tgcttgtttc cttccgccag ccgcaactgc acgccgccgc
     1501 gtggctctcg tcgccgctgt ttgccggcgg cggagcggcc gggcaagcgg ccgaggtcgt
     1561 gcggctgggg ccttacgcgc tcaatgttca gacgccgcag caagtgagct tcatccgtca
     1621 gcccgactat tcgctggccg gcccctcgca gccggcagag gtcgtcgaac ggacgtatcg
     1681 cgactcggcc gttgccctgc gggcgctgcg gcggggtgaa atcagcgtca tcgaccgcat
     1741 cagcccgtgg gagctgacgc ggcttgcgag cagcggcgac atcaaaatcg agccgtacgc
     1801 tttgccgcga gtccatgtgc tattgcccaa ttcgcatcgc tcattgacgg cgaaccggac
     1861 tttccggcgg gccattgcgt atgcaatcga caacgtcgag acgctgaatc gcggcctttt
     1921 ggacggacag aaggttgccg gctgcgaagc gctgagcgga cctttcccgc gcggaccggc
     1981 ttataacgaa gaagtggcga tccgtccgtt cgatcccggc ctggcgatga cgctggcccg
     2041 catgacggcc gacgaggtga acgccgcccg ccaggatcgc ggcgagcccc cgctcgaaat
     2101 gccgcccaag ctcgtcctcg cccatcccgc cgagccgatc gcccgcatcg cctgccagtc
     2161 gatcgcccgg cagcttcggc tcgctgggct gatggtcacg cttcgcgagc aggagcccgg
     2221 ccaggccgcc ggtgaggacg ttgatttgct gtacgccgac ttcgcgatgc acgagccggt
     2281 cgtcgatgcc tggcggctgc tcggacccgg cggagcggtc ggcgattgca gcccggcgat
     2341 gctggcgctg ttgcggtcgc tccaggcggc gaccgatgag caacaggcgc tcgccgcgct
     2401 gcacgaaatc caccgcctgg ccgccgccga attgccggca attccgctct ggcagcttac
     2461 cgaccatttt gcctggcaca cgagcgtgca ggggataggc cagcgaccag tgacgctgta
     2521 cgagaacatt gagcaatggc aggtggagtt tcggattccg cagcagtagt tgatgcacca
     2581 gcggcaggca ggaaaaccac gaatgacacg aatcgtacga atacggcata cggtgcaggc
     2641 cgccttgatg accctattgg tcaccacttc tgcggatgca agtggggctg agccgtggga
     2701 gctgacgccg tatcgcatca agctgctggt ggcggtcgag gcagcgggtg ggctgccgcg
     2761 ggatatcgaa agcgtgttgg cccaggacct cgtcgcgcgt ggcgcagcgg tcgtgggcgg
     2821 ggcttggcga gtggatgcat ccgccgcgcc ggccgaattg cggcacaagc tgatccacgc
     2881 catcgatacc gtctcggcca acgaaatcgc cggcagcgcc ggcgaatacg acaaggtgat
     2941 cctggtcggc gccctgcagg agagcggcaa agtcctgatc caggcgcggg agctggatct
     3001 cgtgacggaa ttatggaacg tcgttgtccg ccgcgatgcg ccgcaggccg agctggttcc
     3061 gcagacggcg tttgcggccg tgctgcaagc tttcgccccg ctggcgcgga tcgaatcggc
     3121 caatggcgag acggcgacgc tgcggttgcg ggccggcggc ctggcccggc gcgatcgtcg
     3181 gctgcccgcg cttgcgccag gcgttgcctt ccggccggtg ctggtcaaga gcgatgcgag
     3241 cggcacactg acgcgcggct cagccgaggc cctggccaac gtgtacctct ctccgaccaa
     3301 cacccgcggt gcggtggtca cctgccgcgt cgatgccggc gcggccgccc cgctgattcc
     3361 cgaataccat ccgcaccgcc agcggctcgc gctggcggtc tctccctcga gcgagtccac
     3421 gcagcttcgg ctggtgagca tcggcgatcc gcccgcgccg ctggaggggc tcgacgtgct
     3481 cgaccggggc gagctgctgg gccgcagcga tcgccgcggc ctggtgacga ttgctccgga
     3541 cgccgaggcg gtgcgcgcgc tgacgatccg ccgcggcgaa cggacgctcg ccacgct
//
LOCUS       DASUGB010000050         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1673892, whole genome shotgun sequence.
ACCESSION   DASUGB010000050 DASUGB010000000
VERSION     DASUGB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673892"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..151)
                     /locus_tag="VFV87_00955"
     CDS             complement(23..151)
                     /locus_tag="VFV87_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102348.1"
                     /translation="MIGLGIAEIIIIALVGLLLLAVIGGVVAAVVIAFSSRGRERD"
     gene            226..705
                     /locus_tag="VFV87_00960"
     CDS             226..705
                     /locus_tag="VFV87_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX5102349.1"
                     /translation="MAKSQKFWLLKTEPESFSIQDLAKAPNQTTCWDGVRNYQARNFM
                     RDGMRVGDRVLVYHSSTDVPAVVGTAKIVRQAYPDHTAWDPKDSHFDPASTPEYPRWF
                     MVDIRLERMFKQPLPLEKLRHTPALKKMELLRKGSRLSVQPVTKSEFAAVLKFAGAK"
     gene            987..>2246
                     /locus_tag="VFV87_00965"
     CDS             987..>2246
                     /locus_tag="VFV87_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5102350.1"
                     /translation="MPRTSRRPLLLFLAAAALLLAMAFLARPVVHVVRSLVRDEEYVS
                     ELPPGHVDDASRLNRTQVAEVWDVPIDAEEPERQLAGLLARAKAEGRRVSIAGARHSM
                     GGHTIYPSGININMLPLNRMEFDEQREILKVGSGALWKDIIPYLDARGRSVAVMQSNN
                     AFSVGGSLSVNCHGWQFDRPPIASTVESFRLMQADGTVVRCSREENQELFSLVLGGYG
                     LFGIILDADLRVVPNERYRLEQFVVPVDRSLATFDAEIKDQPDMRMVYARMNIVPDRM
                     FQDVIINAFFREPGGEIPPLEQAGMADLRRAIFRGSADSDYGKELRWSAETRLQPLLA
                     GKAFSRNQLLNEGTEIFQNRTDSTTDILHEYFLPRDRASGFVRKMREILSERKANLLN
                     VTVREVNTDPDTFLRYADQPMLAFVMLY"
BASE COUNT          437 a          700 c          675 g          434 t
ORIGIN      
        1 ccatcgaggt gatctccacg gttcagtccc gctcgcgtcc gcggctcgaa aacgcaatga
       61 cgaccgcggc gactacgccg ccgatgacgg ccagcaataa caatccgaca agtgcaatga
      121 taatgatttc cgcaatgccc aggccaatca tgatcgtgct cccggcgata gaatcaggta
      181 gctcagctcc tcattcgttg actcaagctc caaattgtag tcctcatggc caaatcacag
      241 aaattctggc tcctcaagac cgagccggag agcttttcga tccaggacct ggcgaaagca
      301 cccaaccaga cgacgtgctg ggacggcgtg cggaactatc aggcccgcaa cttcatgcgg
      361 gacgggatgc gagtcggcga ccgcgtgctg gtgtatcaca gcagcaccga cgtgcccgcg
      421 gtcgtcggaa cggccaagat cgtccgccag gcctatcccg atcacaccgc ctgggacccg
      481 aaagactcgc acttcgaccc tgcttccacg cccgagtacc cgcgctggtt catggtcgat
      541 atccgcttgg agcggatgtt taagcagccg ttgccgctgg aaaagctccg ccacactcct
      601 gcactgaaga agatggaact gctccgcaaa ggctcccgcc tcagcgttca gccggtgacc
      661 aagagcgagt tcgctgccgt cttgaaattc gccggcgcga agtgagtcgc cgtttcccgg
      721 aatcctttgt tggtctgcta cgatacttgc tattctgctc atccagtctt ttggcttcgc
      781 ctgcgggtga tttcaatgcc tgagcctagc atcgtcaaaa ggcctgagat gatctttgtc
      841 ggtcttgagg cggcatttaa tcacgcgctc tccaagaact cgacagcggc ggcggtcatc
      901 ggcccgcttt gcgagcggat tgcaagagag ccacctccga actggagtat tggatttcca
      961 tcgtacccaa aggaggatga gccgcgatgc cccggaccag ccgccggcct ctgttgctct
     1021 ttcttgctgc ggctgcgctc ctgttggcga tggcgttcct cgcgcggccg gtggtccatg
     1081 tcgtccgcag tctggtgcgc gacgaagagt acgtatcgga gctgccgccg gggcatgttg
     1141 acgacgccag ccggctcaat cgaacgcagg tggccgaggt gtgggacgtg ccgatcgacg
     1201 cggaggagcc ggagcggcag ctcgccggcc tgctggcccg cgccaaagcc gaagggcggc
     1261 gcgtgtcgat cgccggcgcg cggcacagca tgggcgggca cacgatctat ccgagcggca
     1321 tcaatatcaa catgctgccg ttgaatcgga tggaattcga cgaacagcgc gagattctca
     1381 aggtcgggtc cggcgcattg tggaaggaca tcattccgta tctcgacgct cggggacgaa
     1441 gcgtcgcggt catgcagtcg aacaacgcct tctccgtcgg cggatcgctc agcgtgaatt
     1501 gccacggctg gcagttcgat cggccaccga tcgcctccac ggtcgaatct ttccgcctga
     1561 tgcaggccga cggcacggtc gtccgctgca gccgcgagga gaaccaggag ttgttctcgc
     1621 tcgtgctcgg cggctatggg ctgtttggca tcatcctcga tgccgacctg cgggtcgtgc
     1681 ccaacgagcg ctaccggctg gagcagttcg tcgtgccagt cgatcggtcg ctggcgactt
     1741 tcgacgcgga aatcaaggat cagccggaca tgcggatggt ctatgcgcgg atgaacatcg
     1801 tcccagaccg gatgttccag gacgtcatca tcaacgcttt cttccgcgag ccgggaggcg
     1861 agattccacc gctggagcaa gccggaatgg ccgacttgcg gcgggcaatc ttccgcggct
     1921 ccgcggacag cgactatggc aaggagttgc ggtggagcgc cgagactcgc cttcagccgc
     1981 tcttggcggg caaggcgttt tcgcggaatc aacttctaaa cgaagggacc gagatctttc
     2041 agaatcgcac cgacagcacg accgacatcc tgcacgagta ctttctgccc cgcgaccggg
     2101 cttccggatt cgtccgcaaa atgcgcgaga tcctcagcga gcgcaaggca aacctgctca
     2161 atgtgacggt tcgcgaggtc aacacagacc ccgacacgtt tctccgttac gccgaccagc
     2221 cgatgctcgc gttcgtcatg ctctat
//
LOCUS       DASUGB010000051         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1257315, whole genome shotgun sequence.
ACCESSION   DASUGB010000051 DASUGB010000000
VERSION     DASUGB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257315"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFV87_00970"
     CDS             complement(<1..611)
                     /locus_tag="VFV87_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5102351.1"
                     /translation="MPHSHDHHHARSTSKTRLVFALVLACAYMLAEVIGGLATGSLAL
                     LADAGHMLSDAAALGLSLAALWIAERPADSKRTYGYYRAEILAALVNGAALGAIAIYI
                     FIEAIRRLAEPTQVLGGWMLVVAVGGLVVNLVMLAILSGGRGESLNIRGAWLHVLTDT
                     LGSIGAIAAAALIWLYRWNWIDPAISIAIALLVIYSGWRLMLES"
     gene            complement(769..1119)
                     /locus_tag="VFV87_00975"
     CDS             complement(769..1119)
                     /locus_tag="VFV87_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102352.1"
                     /translation="MAKKKTAKKKPAKKAVKKAAARKAKKKPKKISLGRPTVTAEEKL
                     FMLFHEDYEARQVFEFLRAETVADLEQHSPQDIIHRLSRPIRETVDRIRKKLADRNRC
                     LTGDEQFAIEHKTR"
     gene            complement(1160..2287)
                     /locus_tag="VFV87_00980"
     CDS             complement(1160..2287)
                     /locus_tag="VFV87_00980"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5102353.1"
                     /translation="MPHLPIVSPAEIGLDERRLQSAYDRLEEWTTGKDAPVPCGAILV
                     GRHGKTVEPRLFGRQSFEPDAPRIRQDAIFLLASITKPVVYMGALMLVERGLLNLTDR
                     VTRYIPDFAAHHKEETLVQHLFTHTSGMPDMLENDRELRAAHAPLSKFIEHAIRDTVP
                     LFPPGTGFSYQSMGTLVTAEIVQRITGQSIHDYVHRQILEPLGLRSTRLGSKGLDDAR
                     IVRNQTPDWQKPDFGWNSQYWRELGVPWGTMFSTPQDFAVICQMILSGGEYDGVRILS
                     PNTVRMMTTNRLDDCPDLPEGIRRTAPWGLGWKLNDPGTPEPLCELLGRQAFGHTGST
                     GNLLWMDPATGGFCILFTTAERARAPWRLIHLSNAVASAFL"
BASE COUNT          420 a          778 c          874 g          474 t
ORIGIN      
        1 gattcgagca tcagccgcca gccggaatag atgaccaaga gggcgatggc gatcgagatc
       61 gccgggtcga tccagttcca gcggtagagc cagatgagag ccgcggcggc aatcgctccg
      121 atgctgccga gtgtgtcggt cagcacgtgc agccacgccc cgcggatgtt gaggctttcg
      181 ccgcggccgc cgctcaggat ggcgagcatc acgaggttca cgaccaggcc gccgaccgcc
      241 acgaccagca tccagccgcc gaggacttgc gtcggctctg ccaggcggcg gatcgcctcg
      301 atgaagatgt aaatcgcaat cgcgcccagg gctgccccgt tgaccagcgc ggccagaatc
      361 tccgcccggt agtagccgta ggtccgcttg gaatcggctg gccgctcggc gatccatagg
      421 gccgcgaggc tgaggcccaa agccgccgcg tcggagagca tgtgtcccgc atcggccaga
      481 agggcgagcg agccggtggc cagccctccg atcacttcgg ccagcatgta ggcacatgcc
      541 aggaccagcg cgaacaccag gcgcgtcttg ctggtgctgc gggcgtgatg atggtcgtgg
      601 ctgtgcggca tgacgctttc ccggtagggt gggacaaagc cgccgcggcc gcaggcccac
      661 caggatgacg cgcatcatgg tgggcctgcg ctcgcttcgc tcgctggtcc caccttacgg
      721 ccgctcgtct cgctccgcga gacgacggca tcgcgcggag cgtgatgcct accttgtctt
      781 atgctcgatc gcgaactgct catcacccgt caggcagcgg ttgcggtcgg cgagcttttt
      841 gcggatgcga tcgaccgttt cgcggatcgg gcgcgagagg cggtggatga tgtcttgcgg
      901 cgaatgttgt tcaaggtccg cgaccgtctc ggcccgcaaa aactcgaaaa cctgccgggc
      961 ttcgtagtcc tcgtggaaga gcatgaagag cttttcctcg gcggttacgg tcggccggcc
     1021 gagggagatt ttcttgggct tcttcttggc cttcctggcg gcagcttttt tgacggcttt
     1081 cttagctggc ttctttttgg cggttttttt cttggccatg gtttattcgc ggggcacgga
     1141 acgccacgga gggcgttccc tacaggaaag ctgacgcaac cgcattcgac aggtgaatca
     1201 gccgccaggg ggcgcgggcg cgttcggcgg tggtaaacag gatgcaaaag ccgccggtcg
     1261 ccgggtccat ccacagcagg ttgcccgtcg agccggtgtg gccgaaggcc tgccggccca
     1321 atagctcgca gagcggctcc ggcgtgccgg ggtcgttcag cttccagcct aatccccagg
     1381 gggccgtgcg gcgaatgccc tccggcaaat ccgggcagtc gtccagccgg ttcgtcgtca
     1441 tcatccgcac ggtattcggc gagagaatcc gcacgccgtc gtactcgccg ccactcaaga
     1501 tcatctgaca gatgacggca aagtcctggg gcgtgctgaa catcgtgccc cacggcacac
     1561 ccagttcgcg ccagtactgg ctgttccagc cgaagtcggg cttctgccag tcgggcgtct
     1621 ggttgcggac gatccgggca tcgtccagcc ccttggatcc caggcgtgtg gagcggagcc
     1681 cgagcggctc gaggatctgg cggtggacgt agtcgtgaat cgactggccg gtgatccgct
     1741 ggacgatttc ggcggtgacc agcgtcccca tgctctggta gctgaagccg gtgcccggcg
     1801 gaaagagcgg gacggtgtcg cggatcgcat gctcgatgaa ctttgagagc ggggcgtggg
     1861 cggcccgcaa ttcgcggtcg ttttcgagca tgtcgggcat tcccgacgtg tgcgtgaaga
     1921 gatgctggac gagcgtctct tccttgtggt gggcggcgaa atcggggatg tagcgcgtca
     1981 cgcggtcggt caggttgagg agcccccgct cgacgagcat caaggcgccc atatagacga
     2041 ccggcttggt gatcgaagcc agcaggaaaa tcgcgtcctg gcggatgcgc ggggcgtccg
     2101 gctcgaagct ctgccggcca aatagccgcg gctcgaccgt tttgccatgc cggccgacga
     2161 gaatcgcccc gcacggcacc ggagcatcct tgccggtcgt ccattcctcc aggcggtcgt
     2221 aagccgattg cagacgccgc tcgtcgaggc cgatctcagc cggactgacg atgggtaggt
     2281 ggggcatggg agtgctccgt gcgggtgaac ttggggcagc aggagccaaa ctagctgctc
     2341 gccgcggcga aaacaacgcg gttggccgag cctgttcttt gccgtggcct gggcgttacg
     2401 atatgcagat gcgctattcg ctgcggattc tgtttgccgc gatcacgatt gtggcggcca
     2461 cgtgcgccaa ttgggccaac tatcgacggg cgacgctctg ccatactcgc tggattgacc
     2521 cggcgtctgc agaggcgcgg aagttg
//
LOCUS       DASUGB010000052         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_423604, whole genome shotgun sequence.
ACCESSION   DASUGB010000052 DASUGB010000000
VERSION     DASUGB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423604"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1126)
                     /locus_tag="VFV87_00985"
     CDS             complement(23..1126)
                     /locus_tag="VFV87_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102354.1"
                     /translation="MDAAPQRRSSTPPHPARKDFLVILLALTAPGSADAASVTTGDVI
                     YACDFDHRAQEAAEGAAGTRGADLDYDGWPDGWSRLRSLKLPEFVRVGIVAEGQTIAL
                     ATAQPAAETVNRVLRMELDGGGATVQSQPVAISPEYSLHLSLRIKTTALAHDGAWATL
                     LLLDADNKILQSVSSRPVSETGDWLRVEIGPITPDSRKVVQAAISLHVGPLGTEQDLT
                     GSAEFDDVQLVRLPRMTLQANSPTGLYVYPATPVFTCDVSGIRIAQPEVRFDLLDYEG
                     QILATQTVPLIPSQSRPVFEATPAPAIEGFTGQATFKPAVPGFGHYRVTASLVSKGDS
                     RGVLGASRSLAVLRPLPPPHLTPCASDSTSPAG"
     gene            complement(1262..2008)
                     /locus_tag="VFV87_00990"
     CDS             complement(1262..2008)
                     /locus_tag="VFV87_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102355.1"
                     /translation="MPLHDWSRVPAGLFHDFHQSWSIRIKDALNAGRLPSGTVALVEQ
                     KSGPLESDVLAIESRGRRRADNEGGGITTIEPPQTQIVRRTTKDFYAGKANRIVIRHH
                     LGRIIAVIEIVSPGNKDSRAAIRDFVEKTIDFLRAGIRVLVIDLFPPTPRDPFGIHKL
                     VWDEIEEEDFAFPPGKDRILASYQTGAERLAYVEPVAIGDQLRDMPLFLANDYHVDVP
                     LESTYLATWNASPEEFRTAVETGKLPESEE"
     gene            complement(2051..>2653)
                     /locus_tag="VFV87_00995"
     CDS             complement(2051..>2653)
                     /locus_tag="VFV87_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5102356.1"
                     /translation="AAADAMAHRLAIPSREGLIRNRYAGRTFIEGGNRVKKAEAKYTP
                     LREVLQGKRIFLVEDSIVRSTTMRVLLNRLRTIGGAKEIHVRVACPPIVAPCFYGIDM
                     STVDELFAPKFLRGGELTDEIQVEMAKALGCDTLRYLPVDSIARAIQLPADNLCRACI
                     TGRYPTPHGQRLHQIAVENVGKTGCDRRTYEVQHALTFAQ"
BASE COUNT          462 a          846 c          865 g          480 t
ORIGIN      
        1 cctcctaggt cgagcccgcg ggttatccag cgggcgaggt ggagtcggaa gcgcagggcg
       61 tcaggtgcgg cggcgggagg ggcctgagga ccgccaggct gcggctggcg cccagaacgc
      121 cgcgcgaatc ccccttggaa accagcgaag cggtcacgcg gtagtgtccg aagccgggaa
      181 ccgccggctt gaatgtcgct tgcccggtaa agccctcgat ggccggcgca ggggttgcct
      241 caaaaaccgg gcggctctgg gaaggaatca gcggcacggt ttgcgttgcc aggatctgcc
      301 cctcgtagtc gagcaggtcg aaccgcactt ccggctgagc gatgcgaatg cccgagacat
      361 cgcacgtgaa tacgggcgtc gccgggtaca cgtagaggcc cgtcgggcta ttggcctgca
      421 gcgtcattcg cggcagccga acaagctgca cgtcgtcgaa ttcggcgctg ccagtcaggt
      481 cttgctctgt gcccagcggc ccgacgtgca gcgaaatggc cgcctggaca accttgcggc
      541 tgtcgggagt gattgggccg atctccacgc gcagccaatc gccggtctcg ctcacgggcc
      601 tgctggaaac cgattgcagg atcttgttgt cggcgtccaa tagcaggagc gtcgcccacg
      661 cgccatcgtg cgccagagcg gttgtcttga ttcgcagcga aaggtgcagg ctgtactcag
      721 ggctgatcgc gaccggctga ctttggaccg tcgccccgcc gccgtcgagt tccatgcgca
      781 gcacgcgatt gacggtttct gccgccggct gcgcggtggc aagggcgatc gtctgccctt
      841 cggcgacaat ccccacgcgc acgaactccg gcagcttgag cgaccgcagc cgcgaccatc
      901 catccggcca gccgtcatag tccagatccg ccccgcgcgt gccggcagct ccttccgccg
      961 cctcctgcgc gcgatgatcg aaatcgcagg cgtagatcac gtcgcccgtc gtgacggagg
     1021 ccgcatcggc tgagcccggc gctgtgaggg ccaggagaat gacgaggaaa tccttgcggg
     1081 cggggtgggg aggggtcgaa gaacggcgct gcggcgcagc gtccatagaa tccttgctta
     1141 tccccgcacc caagctgacc aatcccgcct gaaagctccg ccgtttgtgc caaccgtgcc
     1201 ggatgtacgg gctgtgccgt gctggatcgc cgccggcgtc acctcctttg cgaacccgat
     1261 attactcctc ggattccggt agcttgcccg tttccaccgc ggtgcgaaac tcttccgggc
     1321 tggcgttcca agtggccaga taggtcgatt ccagcggaac atcgacgtga tagtcgttgg
     1381 ccagaaagag cggcatgtcg cgcagctggt cgccgatggc aactggctca acataagcca
     1441 ggcgctcggc gccggtctgg tacgatgcca gaatgcggtc cttgccgggc ggaaaggcga
     1501 aatcctcttc ctcaatctcg tcccaaacga gcttgtggat gccaaacgga tcgcgcggcg
     1561 tgggaggaaa aagatcgatc acgagcacgc ggatgcccgc ccgcaaaaag tcgatcgtct
     1621 tctcgacgaa gtctcggatg gcggcccggc tgtccttgtt tcctggagag acaatctcaa
     1681 taacggcgat gatccggccc agatggtgcc ggatgacgat gcgattggcc ttgccagcgt
     1741 aaaagtcctt cgtggtgcgc cggacgattt gggtctgcgg cggttcaatg gtcgtgatcc
     1801 cgcctccctc gttgtccgcc ctccgacgcc cgcgcgattc gatcgccagc acgtcggatt
     1861 ccagtggtcc gctcttctgc tcgaccagcg ccaccgtccc cgaaggaagc ctgccggcat
     1921 tcagagcatc cttgattcgg atcgaccagc tttgatgaaa atcgtgaaac agtccagcgg
     1981 gaacgcggct ccagtcgtgc agcggcatcg ctctgccctc ctgcgacaat tataacctgc
     2041 ccgatgcccg ttactgtgcg aacgtcagcg cgtgctgcac ttcgtaggtt cgccggtcgc
     2101 agccggtttt gccgacgttc tcgaccgcga tttgatgcag ccgctggccg tgcggcgtgg
     2161 gatagcggcc agtgatgcag gcccggcaga ggttgtcggc gggcagttgg atggcccggg
     2221 cgatggaatc gaccggcaga tagcggagcg tatcgcagcc gagggctttg gccatttcga
     2281 cctggatttc gtcggtcagt tcgccgccgc gaaggaactt gggcgcgaac agctcatcga
     2341 cggtgctcat atcgatgccg taaaagcacg gcgcgacaat cggcgggcag gcgacgcgga
     2401 cgtgaatctc cttggcgccg ccaatcgtgc gcaggcggtt gagcagcacc cgcatcgtcg
     2461 tcgagcggac aatggaatcc tcaaccagga aaatccgctt gccctgcagc acctcgcgga
     2521 gcggcgtgta cttcgcctcc gccttcttca cgcggtttcc gccttcgatg aacgtgcggc
     2581 ccgcatagcg gttgcgaatc agcccctcgc gcgacggaat cgccagccgg tgggccatcg
     2641 cgtcggcagc ggc
//
LOCUS       DASUGB010000053         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1118246, whole genome shotgun sequence.
ACCESSION   DASUGB010000053 DASUGB010000000
VERSION     DASUGB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118246"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFV87_01000"
     CDS             <1..767
                     /locus_tag="VFV87_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5102357.1"
                     /translation="TATAQPKYHEGLGPLVAGFVYAPFGDLEAAAKLVDEETCAILVE
                     PIQGEGGVRIPPAGFLQGLRKLCDDKGLLLIFDEVQTGCGRTGDWFAYQHFGVTPDVM
                     TLAKALCGGIAGGALLAKPEIAPSLRPGMHAATFGGNPIAARAGIATLEMIEGEGLLD
                     NAKQLGELFRRRLLALQEECDLVREVRVAGVMIGIELAVEGSPVVKDCLDRKLLVNCT
                     HNTVIRLLPAMNLTEEQAEEGCDILAGAIKNLSRPA"
     gene            892..1809
                     /gene="argF"
                     /locus_tag="VFV87_01005"
     CDS             892..1809
                     /gene="argF"
                     /locus_tag="VFV87_01005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5102358.1"
                     /translation="MKNVLTLNELTTEEILRIFAISKHLKDEFTSGERKPVLAGRTMA
                     LLFDKPSLRTRVSFETAMAHLGGTSLFLGADVGWGKREAIVDFAQVLSQYVDVIVCRT
                     TTHKRVEDLAAYATCPVVNGLTDLAHPCQALADLFTLEEIHSPLEGKRLAYIGDANNV
                     ARSLAVACGKLGVEMAIASPSQYQFERPFLEQLTMNVPEVKLFQTTDPREAVRGAIGV
                     YTDVWTSMGQEAEQEARKKAFANYQVNANLMEHAPEGAVFLHCLPAHRGEEVTDDVMD
                     GHYSAVLVQAANRMHVQKGLLAWLLNAID"
     gene            1815..2528
                     /gene="rph"
                     /locus_tag="VFV87_01010"
     CDS             1815..2528
                     /gene="rph"
                     /locus_tag="VFV87_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5102359.1"
                     /translation="MRKPTELRPVKITRGYTKMSPGSVLYRAGGTVVLCTASVEPKVP
                     DWLKGKGKGWITAEYNMLPHSTSPRKARERAKLDGRTTEIQRLIGRSLRAVADLAALG
                     ERSILVDCDVLEADGGTRTASITGAFIALVDCLRSVQKELPDPAQFPLTSIVAAISVG
                     IVDGKPALDLDYAQDVAADVDMNVVMTGSGRFIEIQGTGEEATFADDDLAALLKLAKA
                     GIKQLTEVQHKALGKHWPF"
     gene            2540..2938
                     /locus_tag="VFV87_01015"
     CDS             2540..2938
                     /locus_tag="VFV87_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102360.1"
                     /translation="MSVPPIVRHMIACEDYEVAPPPSQRLSIHGLLTGLRAIDPFPVL
                     YQGFCVVLVLTDGRGVGDGQIVCVVEETGQPVFGSQVHPIHFSNDPLERVVAPFRLRA
                     CPFPFPAMYSIQFWYNQQLIHECPLRVRSA"
     gene            2935..3303
                     /locus_tag="VFV87_01020"
     CDS             2935..3303
                     /locus_tag="VFV87_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102361.1"
                     /translation="MNPGRLEMEGGAFQEFSSSAVPAQGLGQPAQPGRCEPERFHVLD
                     EFATTVAGPQTDVNSLDYARCKTQTLVEVVRAVGTSITDRNLSVLQDLASARRQESED
                     DAAEQLLQLVAHIRAGQGRS"
     gene            3327..>3619
                     /locus_tag="VFV87_01025"
     CDS             3327..>3619
                     /locus_tag="VFV87_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102362.1"
                     /translation="MIAASTNFAPPPHHRWIRFSLRTLLVFMVAASLALGWIGNVLVR
                     VRHQRQVVRRIEALGGKVDYDYVFDENYGKHAPGPKVLRVVLGDDAFLHVDRV"
BASE COUNT          674 a         1125 c         1149 g          671 t
ORIGIN      
        1 tgactgccac cgcgcagccg aagtatcacg aagggctcgg cccgctggtt gccggatttg
       61 tttacgctcc cttcggggac ctggaagccg cggcgaagct ggttgacgaa gagacgtgtg
      121 cgatcctcgt cgagccgatc cagggtgaag ggggcgtgcg gattccgccg gctgggttcc
      181 tgcaggggct gcggaaactg tgcgacgaca aggggctgct cttgatcttc gacgaggtcc
      241 agaccggctg cggccgcacc ggcgattggt tcgcctatca gcatttcggc gtcacgcccg
      301 acgtgatgac cctcgccaag gccctctgcg gcggcatcgc cggcggcgca ctgctcgcaa
      361 agccggaaat cgcgcccagc ctgcggcccg gcatgcacgc ggctacattc ggcggcaatc
      421 cgatcgccgc ccgggccggt attgccacgc tggaaatgat cgaaggcgaa gggctgctgg
      481 ataacgccaa acagctcggc gagctcttcc gccgccggct gctggcgctg caggaggagt
      541 gcgatctggt gcgcgaagtc cgcgtggccg gcgtgatgat cggcatcgag ctggccgttg
      601 agggttcgcc ggtcgtcaag gactgccttg accgcaagct gctcgtcaac tgcacccaca
      661 atacggtcat ccgcctcttg cccgcgatga acctcaccga ggagcaggcc gaagagggct
      721 gcgacatcct ggcgggcgcc atcaaaaatc tctcccggcc ggcttaacgg ccggcgcttc
      781 accggctctg gtggcggggg ggcccggcgg ccacgcggcg cgtttggtgc tttcgttctt
      841 gtcacggatt tgaactcgaa aacgatcacg gaatcttgag aaggaagtcc catgaaaaac
      901 gtcttgacgc tcaatgaact gaccacggaa gaaatcctgc ggatctttgc gatctccaag
      961 cacctgaagg acgagttcac cagcggcgaa cgcaaacccg tgctggccgg ccggacgatg
     1021 gctctcttgt tcgataagcc ctcgctgcgg acccgcgtca gcttcgagac cgcgatggcc
     1081 cacctcggcg ggacgtcgct gtttctgggc gccgacgtcg gctgggggaa gcgcgaagca
     1141 atcgttgatt tcgcccaggt gctcagccag tacgtcgatg tcatcgtctg ccgcaccact
     1201 acccacaagc gcgtcgagga cctggccgcc tacgccacct gccctgtcgt caacggattg
     1261 accgacctgg cacacccgtg ccaggcgctg gccgatctgt tcacgctgga agaaatccac
     1321 agcccgctgg agggcaaacg gctggcgtac atcggcgacg cgaacaacgt ggcccgcagc
     1381 ctggcggtcg cctgcggcaa gctgggcgtg gaaatggcaa tcgcttcccc gtcgcagtat
     1441 cagttcgagc gcccgttcct cgagcagctc accatgaacg tgcccgaggt caagctcttt
     1501 cagaccacgg accctcgcga ggcggtccgc ggcgcgatcg gcgtttatac cgacgtctgg
     1561 accagcatgg ggcaggaagc cgagcaggaa gcccgcaaaa aggcgttcgc caactaccag
     1621 gtcaacgcca acctgatgga gcacgccccc gagggcgccg tcttcctgca ctgcctgccg
     1681 gcccatcgcg gtgaggaagt gaccgacgac gtcatggacg gccactacag cgccgtcctc
     1741 gtccaagctg ccaaccggat gcacgtgcag aaggggctgt tggcgtggct gctgaatgcg
     1801 atcgactgat ctaaatgcgc aaacccaccg aactccgtcc cgtcaaaatc acccgcggct
     1861 acacgaaaat gtcgccgggg agcgttttat atcgcgcggg ggggacggtg gtgctttgca
     1921 ccgcgagtgt ggagccgaag gtgcccgatt ggctcaaagg caagggcaag ggctggatta
     1981 cggccgaata caacatgctg ccgcattcga ccagcccgcg gaaggcgcgc gagcgggcca
     2041 agctcgatgg ccgcacaacc gaaattcagc ggctgatcgg gcggagcttg cgggctgtgg
     2101 ccgatctggc ggcgctcggc gagcggtcga tcctggtcga ttgcgacgtg ctcgaagccg
     2161 acggcggcac gcgcaccgcc agcatcaccg gggccttcat tgccctggtc gattgcctgc
     2221 gctcggtgca aaaggagctg cccgatcctg cccaattccc gctcaccagc atcgtcgccg
     2281 caatcagcgt cggcattgtc gatggcaagc cggcgctcga cctcgactac gcgcaggacg
     2341 tggcggccga cgtcgatatg aacgtcgtca tgaccggcag cgggcggttt atcgaaatcc
     2401 aaggcaccgg cgaagaagcc acgttcgccg acgacgacct cgccgccctc ctcaagctcg
     2461 ccaaagccgg catcaagcag ctcaccgaag tccagcacaa ggccctcggc aagcattggc
     2521 cgttttgagg gagacatcca tgtcggtgcc gccgattgtc cgtcatatga ttgcatgtga
     2581 agactacgag gttgcaccgc caccaagtca gcgattgtcg atccacggat tgttgaccgg
     2641 tctgcgggcg attgacccgt ttccagtgct ctaccaaggc ttctgcgtag tgcttgtcct
     2701 gacggatggg cgcggcgtgg gcgatggcca gattgtgtgc gtggtggagg agacggggca
     2761 gcccgtattc ggttcgcagg tgcaccccat ccactttagc aacgatccat tagagcgagt
     2821 ggtcgcgcca tttcgtctgc gcgcctgccc ctttcctttt cccgcgatgt attcgatcca
     2881 attctggtat aatcagcagt tgatccacga atgtcctctg cgcgtgaggt cagcatgaat
     2941 cccggccgtc ttgaaatgga aggcggggcc ttccaggagt tttcgtcctc tgccgttcct
     3001 gcccaaggtt tgggccagcc cgcgcagccc gggcgctgcg agccagagcg gtttcatgtt
     3061 cttgacgagt tcgcgacgac cgtcgcgggg ccacaaacgg atgtaaacag cttggactat
     3121 gcgcgctgca agacccagac gctcgttgag gttgtcaggg ctgtcggaac ttcgatcacg
     3181 gatcggaacc tgagcgtgct gcaagacctg gcttcggctc ggcgccagga atccgaggat
     3241 gatgcggctg agcagttgtt gcagttagtg gcgcatattc gggccggtca aggtcggtcc
     3301 tgaaaggatt cggaccaacg cgcagcatga ttgccgcttc gacgaatttc gcaccgccac
     3361 cgcaccatcg ctggattcga tttagcctgc ggacgctgct cgtcttcatg gtcgccgcga
     3421 gtctggccct cggctggatt ggcaacgttc tcgtccgcgt tcgccaccag cgacaggtcg
     3481 tcaggcggat tgaggctctc ggtggcaaag tcgactatga ctatgtcttt gatgagaact
     3541 atgggaaaca tgcacccggt ccgaaagttc ttcgagtcgt actgggggac gacgcatttc
     3601 tacatgtcga tcgagtgca
//
LOCUS       DASUGB010000054         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1813467, whole genome shotgun sequence.
ACCESSION   DASUGB010000054 DASUGB010000000
VERSION     DASUGB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813467"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFV87_01030"
     CDS             <1..398
                     /locus_tag="VFV87_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102363.1"
                     /translation="WKMQNTVGTYLFDSSTQQLNSAGEHLVKWIVQQAPQHRRAVFVL
                     KADTPEDTEVRVASVQAAVTRFACAGENPPIILTMTEPAGWPASYVDQMTQSFQATMP
                     APRLPSSGGEGNGEVNGNSGGGGSGNGSN"
     gene            733..1839
                     /locus_tag="VFV87_01035"
     CDS             733..1839
                     /locus_tag="VFV87_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5102364.1"
                     /translation="MSRDQGGNDVDIKRQLSSIALCAGIGAGLIGCRSSMGGLAFWNS
                     KQDTSIGRASPDVGKQKYDTLAKEFSGSGPSATALGGRQPAEEKGFAGVWKSTTSSVA
                     GVFSSKSEVEADPDVTRLDAPSKKVGPEVHLAAAQLLENQSKFEEAEEHYAKALKVAP
                     NDLHALVGLARMYDRQGQAAKSQQVYERALKAHPKSALVYNDLGLCYARQRDFQRSLG
                     SLGKAVELQPDNVRYRNNLATVLVEAGRTDEALALLCQTGSEAVAHYNVGYLLHKQGN
                     AIAATQHMQRAIALDPGLTAAREMLAQWQGARPMSGGEVNAATVAARPRYDAYGAGVP
                     AAPISIQIPSTVPPRSTEAVHVDDGASFRLPPVK"
     gene            2008..2742
                     /locus_tag="VFV87_01040"
     CDS             2008..2742
                     /locus_tag="VFV87_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102365.1"
                     /translation="MRCAILVGAFLCGLGLIGCSGSSGTSTTADTDGANSSATPSQGV
                     AAAAAAKPVSVPADAAPDQVVAVFLDALRGGDQATTATLLTAKALAETSKENLSVCPQ
                     ATPHMQYQIAESQVLPDNPNGAHVKVVWTENYTDGTVSYDVVWVLRRQQEGWRIAGMA
                     IELVPGRGLAFLNFEDPQDMLRKQQEAELEIEKAMAAAYPPAAETAALPAAPPVEKPS
                     TASPPDGEFTPNSAFPSSPAIQQVER"
     gene            complement(3056..3496)
                     /locus_tag="VFV87_01045"
     CDS             complement(3056..3496)
                     /locus_tag="VFV87_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102366.1"
                     /translation="MAAAITMAAAVTMATVATAAAARARTAIRSRAAIAAIARRMATM
                     AATATEQAGFGLILTAHKGDPNQREKDRDTENNDTVHPRILQLLTGTGKRENTQDAVT
                     CFCLAARVGVREKRTLRSLRETAPSISQPLSSFEGYEGCGDRTG"
BASE COUNT          692 a         1132 c         1131 g          691 t
ORIGIN      
        1 gctggaagat gcagaatact gttggcacat atctgttcga ttccagcacg cagcagctca
       61 atagcgccgg tgagcatctc gttaagtgga tcgtccagca ggccccgcag catcggcggg
      121 cggtatttgt gctgaaagcc gatacccccg aggacaccga agtccgcgtc gcatcggtcc
      181 aggccgcggt cactcgcttt gcctgcgccg gcgagaatcc acccatcatt ctgaccatga
      241 cggaaccggc cggctggccg gcatcgtacg tggatcaaat gactcagtct ttccaggcaa
      301 cgatgcctgc tccacgcctc ccgtcgtcgg gtggcgaagg aaacggcgaa gtcaacggca
      361 acagtggcgg cggcggttcg ggcaacggga gcaactaggc tgtcccgact gacgccttga
      421 attgccggag aagtcgcact cgctggcagg gccccaatgg ccgacagtcc catcggctcc
      481 ccttaaggtg gaggggggcc gcctgcgata acggcaccta aacactcgct cgtccctcgc
      541 tgcgacgatg cctctcggtc gagtccctgc cactgctagc tcgtcgaact cgcgtaaggc
      601 aattcagcgg acgattttgc gctacagcgg ccatcggccg ggcgacgatg aagacacaaa
      661 ggctgcgtgg attttgcggc cagtctggaa gggcctgcag cagcagatca caggctttgc
      721 aggcccatcg gaatgtcccg ggatcaggga ggcaacgacg tggacatcaa acggcagctc
      781 tcatcaatcg cgctttgtgc aggaataggc gccggcctga tcggctgccg gtcttcgatg
      841 ggaggcctgg cattctggaa ctcgaagcaa gacacgtcca ttgggcgcgc gtcgcccgac
      901 gtgggcaagc agaaatacga tacgctcgcc aaggaatttt ccggatccgg ccccagtgcg
      961 acggcgctcg gaggccgcca gccggcggag gagaaaggct ttgccggggt ctggaagagc
     1021 acgacgtcct cggtcgcagg cgtcttttca tccaaatcgg aagtggaagc ggatccggac
     1081 gtgacgcgcc tggacgcccc ctcgaagaag gtcggccctg aggtgcatct cgcggccgcc
     1141 caactgctgg agaatcagtc gaagtttgag gaggcagaag agcactatgc gaaggccctg
     1201 aaagtcgcgc cgaacgactt gcacgccctg gtcgggctgg cccgcatgta cgatcgccag
     1261 ggtcaagcgg caaagtcgca gcaggtttat gagcgggcgc tgaaggccca tcccaagagc
     1321 gcactcgtct ataacgacct gggcctttgc tacgctcggc agcgcgactt tcagcggtcg
     1381 ctcggttcgc tgggaaaggc cgttgagttg cagccagaca acgtccgcta tcgcaacaac
     1441 ctggcgaccg tgctcgtgga agcaggtcga accgacgagg cgctggccct cctgtgccaa
     1501 accggctccg aagccgtggc ccactacaac gtgggatact tgctgcacaa acagggcaac
     1561 gccatcgcag cgacgcagca catgcagcgg gccattgcac tcgatcccgg gctgactgct
     1621 gcccgcgaga tgctggccca gtggcagggg gcgcgcccca tgagcggcgg tgaggttaat
     1681 gcggcgaccg tcgccgctcg gccgcgttac gacgcgtacg gggcgggtgt tccggcggct
     1741 ccgatctcga ttcaaatccc ctcaactgtg cccccacgca gcacagaagc cgttcatgtc
     1801 gatgacggcg cctcgttccg gctgccgccg gtgaaatgac gcgcagttcc gcgtgatatt
     1861 cgcagctagc agggcggcga ttcgtcttga cgcgggtcgg gctacctgag acattctgcc
     1921 gcgctggcag gacgaattgg cgtgcctccg cgcgcgaaca cccttgccga cggtcaagga
     1981 cggacacgtt ttttggagtc tcggacgatg cggtgcgcaa tcctggttgg agcatttctt
     2041 tgtgggctgg gattgatcgg ctgctcggga tcgagcggca catcgacaac ggccgatacg
     2101 gacggggcga attcttccgc cacgccatct caaggcgtcg ccgcggctgc tgccgcgaag
     2161 ccggtctccg tgccggccga cgccgcgccc gaccaggtcg tggcggtgtt cctcgacgcg
     2221 ttgcgcggcg gcgatcaggc cacgacggcg actctgctga ctgccaaggc gctagcggaa
     2281 acatcgaagg aaaacctctc ggtttgcccc caggccacgc cgcacatgca gtatcagatt
     2341 gctgaatcgc aagtccttcc cgacaacccc aatggtgcgc acgtcaaagt cgtgtggacc
     2401 gagaattaca ccgatggaac cgtcagctac gacgtggttt gggtccttcg ccgccaacag
     2461 gaaggttggc gcattgccgg catggctatt gaactcgttc ctggaagagg cctggcgttc
     2521 ctgaattttg aagacccgca ggacatgctc cgaaagcagc aggaagccga actggaaatc
     2581 gaaaaggcaa tggcagcggc ctatccgccg gcagccgaaa ccgctgccct tccggcggcc
     2641 cctccggttg agaagccgag caccgccagc cctcctgacg gtgaattcac cccgaattca
     2701 gcatttccgt cttccccggc aattcaacag gtcgagcgat aggggcacgt tctacgccat
     2761 tggcgtacct gatggaaaga atcaggggaa atgaagctcg atcgcagccg agctgcgtct
     2821 ccgcctgatc gctttgacct tccgttcgtg acgcaaagtc ttttggtaca acgatatagg
     2881 acgaatcctt ccgacttctc ttgcggcgca ccttctgcga tccgcgctgg acatcctgca
     2941 atagcagcgt aagtttgtca gcaattgggc tttgcccaat gccccaggtg gccccagtct
     3001 gtgcattttg cctccggccg cttgacaacg gaacgcgcgt tgctacccta acggcctaac
     3061 ctgtgcgatc gccacatcct tcgtaaccct caaagcttga caggggctgc gagatcgaag
     3121 gcgcggtttc tcgcagggaa cgtaaggtcc gcttctcgcg cactcccacg cgagcggcga
     3181 gacaaaagca tgtgactgca tcttgggtgt tttcccgctt accggtacct gtaagtaact
     3241 ggaggattcg aggatgaacc gtatcgttgt tctcggtgtc gcgatctttt tcgcgctggt
     3301 tgggatcgcc cttgtgggcg gtcagaatga ggccaaagcc ggcctgttcg gttgccgtgg
     3361 ctgccatggt tgccattcgt cttgcaatgg ctgcaatggc tgctcgactt cgaattgctg
     3421 tgcgagctcg tgctgcagca gcagttgcca cggttgccat cgtaaccgct gctgccatcg
     3481 taatcgctgc tgccatagcc gttgccacgg ctgcaatggc tgctatagca gctgcaacgg
     3541 ttgcaatggt tgcggcggcg ctgttccggt tccggtcgaa ggtgctccgc cggccccgcc
     3601 cgctccgccg gccccgcccg ctccgccggc tgcttagtgc attccc
//
LOCUS       DASUGB010000055         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1118378, whole genome shotgun sequence.
ACCESSION   DASUGB010000055 DASUGB010000000
VERSION     DASUGB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118378"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1625)
                     /locus_tag="VFV87_01050"
     CDS             complement(183..1625)
                     /locus_tag="VFV87_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX5102367.1"
                     /translation="MTKDDREIVSAVTEALAGRVGRERFELWFGRSVRIEWALGKLRV
                     LAAEAFRLDLLRRAFRADLLAAAMAVLGADVSLEFAVDAALAQESSQPAQQPGEPPMA
                     SRAATLDRPAGAVGARAAAPSRAQRLDGQPRTGKPHWGSLCEFIPGEANRLALTAAQT
                     AAERPGEYSPLTLVGPAGSGKTHLLEGIWRQAREHRTCQRVIYLSAEQFTNQFLDALR
                     QSGTPSFRRKYRDVELLLIDDVQFFAGKQSTLVELVHTIDTLVRDGRQVVCSSDRPPA
                     ELRTLGPELVTRLAGGLVCTIQPADFATRLGILRQIAGRQRMQVPEDVLQWLAGQLDG
                     DARHLAGALNRLRAASDAHERPIDLAFAQDTLDDLIHACRRPVRLPQIVEAVCDVLGV
                     ESDQLQSSSKSPSVTLPRMLIMFLARKHTRAALSEISRTLGRKSHSTVVSAQHTVTEW
                     LAGGKTVPAAHGSLKVEDAIKRVEGRLKLG"
     gene            complement(2003..>2536)
                     /locus_tag="VFV87_01055"
     CDS             complement(2003..>2536)
                     /locus_tag="VFV87_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5102368.1"
                     /translation="YLAAEADSFPEHPLRLPFALAAAANLERASLIDLVDATYIGTLD
                     CPRIDNLRKTADVIEGYQAVGASGSQLWKIVLGENDRRVGCLLIADHPSAKQWEIVYV
                     GLVPEVRGRGWGLELTRHAQWLARQAKVERLVLAVDAANEPAIRIYAAAGFLAWDRRA
                     IWIKPLARGETGLPPTT"
BASE COUNT          439 a          833 c          812 g          452 t
ORIGIN      
        1 gcctccaaac actcgcctgc cttggcacgt ttacccatcc ttgggtcctc gcgtgtggtt
       61 tagttttctc ttggcagaaa aaacataaac ccacgcaggc ccttggactt agatcgattt
      121 gatttcagga caccctcagg ctggagcctg ggaacgaggg ctgatgacgc ccaatctccg
      181 cgctaaccca gcttcaggcg tccttccaca cgcttgatgg cgtcttcgac cttgaggctg
      241 ccgtgcgcgg cggggaccgt cttgccgccg gcgagccact cggtgactgt gtgctgggcc
      301 gagacgaccg tgctgtggct cttgcggcca agggtgcggc tgatttcgga gagggccgcg
      361 cgggtgtgct tgcgggccag aaacataatg agcatccgcg gcagcgtgac actgggcgat
      421 ttgctgctcg actggagctg gtccgattcg acccccagca cgtcgcacac cgcctcgacg
      481 atctggggca agcgcacagg ccgccggcag gcgtgaatca aatcgtcgag agtgtcctgc
      541 gcgaaggcga gatcaatcgg ccgctcgtgg gcgtctgacg cggcccgcag gcgattgagc
      601 gccccggcca gatggcgggc gtcgccatcg agctgcccgg cgagccactg cagcacgtcc
      661 tcaggaactt gcatgcgctg ccgcccggca atctgccgca aaatgcccag ccgcgtggcg
      721 aaatcagccg gctggatcgt gcagaccagg ccgcctgcga ggcgcgtgac cagctccggt
      781 ccgagcgtcc gcagttcggc gggcggacga tcggacgagc agacgacctg ccgcccgtcg
      841 cggacgagcg tgtcgatcgt gtggaccagt tcgacgagcg tcgattgctt gccggcgaag
      901 aactgcacgt cgtcgatcaa gagcaactcc acgtcgcgat acttgcggcg aaagctgggc
      961 gtgccgcttt ggcggagggc atcgaggaac tgattggtga actgctcggc cgagagatag
     1021 atgacgcgct ggcaagtgcg atgctcgcgg gcttgtcgcc agatgccttc cagcaagtgc
     1081 gtcttgccgc ttcctgcggg gccgacgagc gtcaacggcg agtactcgcc gggtcgctcg
     1141 gctgccgtct gggcggccgt gagggcgagg cggttggcct cacccggtat gaactcacaa
     1201 aggctgcccc agtgcggttt gccggtccgc ggctgaccgt cgaggcgctg agcccgagat
     1261 ggagccgccg ctcgagcgcc caccgcaccg gccggtcggt cgagcgtcgc tgcacggctg
     1321 gccatcggcg gttcgcccgg ttgttgggcc ggctggctgc tttcctgcgc cagggcggca
     1381 tccacggcaa attccagcga aacgtctgcc cccagcacgg ccatggccgc ggccaggaga
     1441 tccgcccgaa aggcccgccg caacagatcg agacgaaagg cctccgcagc gagcacgcgc
     1501 agcttgccca gagcccattc gatccgcacg ctgcggccga accaaagctc gaatcgttct
     1561 cttccgaccc tgccggccag cgcttcggtc acggccgaca cgatctccct atcgtccttg
     1621 gtcacttcct gaggcgtcct gtgcgcgcag cgcgagcgcc gaaagtccta gcgagaaaat
     1681 cgcccgtcgc gagaagaccc gcgatcgtct gccggcgcgg cagcgtgccc gatcgagtta
     1741 ccctgatcca cagcggcgga tcatccgtcc gctgctgctc gttgtgcagc gggggtggat
     1801 tttaagaacg ctagcacgaa agtcaacggc ccgcgcgaag agcgtgcaaa gattttcttc
     1861 gcgcgacttt cacgcccgca ctttccaccg taatcgctcg cctgcgccgc gcttgggccg
     1921 aactcttcat gaatttcttc agccgctggt agtccgtgtg taaaacgtgt cgccgcgaat
     1981 gtgaagcccc agcgctcgcc gcctaagtgg ttggcggcaa accagtttcg ccacgcgcca
     2041 gcggcttgat ccagatcgcg cgccggtccc aggcaagaaa gccggctgcc gcgtagatgc
     2101 gaattgccgg ctcgtttgcg gcatcgaccg cgagcaccag cctttcgact ttggcctggc
     2161 gggccagcca ctgcgcgtgg cgcgtcagtt ccagccccca gccgcggccg cggacttcgg
     2221 gaaccagtcc gacatagacg atttcccact gcttcgctga tggatggtcg gcgatcagca
     2281 gacatccgac gcgtcgatca ttctcgccca gaacgatttt ccaaagttga ctgcccgatg
     2341 ccccaaccgc ctggtaaccc tcgattacgt ccgccgtttt ccgcaggttg tcgatccgcg
     2401 ggcaatcgag cgtgccaatg tacgtggcat cgaccaggtc aataaggctt gctcgttcga
     2461 gattggccgc cgccgcgagc gcaaatggca gccgcagcgg atgttcgggg aaagaatcgg
     2521 cttcggcggc taaata
//
LOCUS       DASUGB010000056         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1118713, whole genome shotgun sequence.
ACCESSION   DASUGB010000056 DASUGB010000000
VERSION     DASUGB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118713"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFV87_01060"
     CDS             complement(<1..460)
                     /locus_tag="VFV87_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5102369.1"
                     /translation="MDTHALLLAAAEIYQPLVPLWAMELAFDVFLGMWLFCVGASVGS
                     FLNVVVYRLPRGMNLLYPASRCPSCLHPIRLRDNIPILGWLLLGGRCRDCQARISPRY
                     LVVELIVAGVFLAAWFAEAHFPPGRIAAGAMAYLPHDPSYQALFWCAYGLH"
     gene            603..1250
                     /locus_tag="VFV87_01065"
     CDS             603..1250
                     /locus_tag="VFV87_01065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5102370.1"
                     /translation="MSHLILLRHGESQLNVVNRQRRVYCGQTETPLTDQGREQARQAG
                     RQLAAREGLQIRWAVSSCHERSRETLRLAAGELPYAVELLPPLEGLNERSLGRFEGLA
                     EEDVFRDHPHYRDHPDFRRFMNCFVQKAPGGENLADVTLRAWSAVESLLGESLQAERA
                     GDLLIVSHYNTIRCLLGRLLALSEQAVLDLRVPNAVPIVIRHGSRWELVDGLELP"
     gene            2082..>2656
                     /locus_tag="VFV87_01070"
     CDS             2082..>2656
                     /locus_tag="VFV87_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102371.1"
                     /translation="MNRSRTPFVLAIANLILAMVFGLCPSSGAIAAEPLRIATFEADI
                     TPPLGSPLCDALVQPARTIDDPLLARGIVLFPEGEPIVLCALDWVGIGNSGYDAFREA
                     LANAAGTKAERVAVHCLHQHDAPGCDFAADDLLKPLGLNNELFNAAFAREAMQRIASA
                     VSKACQSPQPVTHISLGKAKVEQVASARRVMG"
BASE COUNT          504 a          783 c          881 g          488 t
ORIGIN      
        1 catgcagccc atacgcgcac cagaaaagtg cctggtagct gggatcgtgc ggcagataag
       61 ccatcgctcc ggcggcaata cgtcccggcg gaaaatgggc ctcggcgaac caggcggcca
      121 gaaacacgcc cgccacgatc agctcgacaa ccaaataccg cggcgaaatc cgcgcctgac
      181 aatcgcggca ccttccgccg agcagcagcc agcccaggat ggggatgttg tcgcgcaggc
      241 ggatcggatg caggcagctc gggcagcgcg aggcgggata gagcaggttc atcccccgcg
      301 gcaggcgata gacgacgacg ttcagaaagc tgccgacgct cgctcccacg caaaacaacc
      361 acattccgag aaagacgtcg aatgccagct ccatcgccca caaggggacg agcggctggt
      421 aaatttcagc ggcggcgagg aggagggcgt gcgtgtccat caagagcagc gcgcgggcaa
      481 tggtcaggcg gcaggcagga atgctgcccc acgattggcg ttcattctgt catggctcgc
      541 gagcccgctg taggcgggtt tggagtacaa tcagggttcg atcccgcctc gcccatcccg
      601 ccatgtcgca cctgatcctg ctccgccacg gagaatcgca gctcaatgtg gtcaaccggc
      661 agcggcgcgt gtattgcggg cagacagaaa cgcctttgac cgaccaggga cgcgagcagg
      721 cccgccaagc ggggcggcaa ctggcggccc gcgaagggct gcagatccgc tgggccgtca
      781 gctcgtgcca cgagcgctcg cgggagacgc tgcggctggc ggcaggcgag ctgccgtatg
      841 cggtggaact gctgccgcca ctggaagggc tgaacgagcg gtcgctgggg cggttcgagg
      901 ggctggcgga ggaggatgtc tttcgcgatc acccgcacta tcgggatcat cccgatttcc
      961 gccgcttcat gaactgcttc gtgcagaagg cgcccggagg cgagaacctg gcggacgtga
     1021 cactccgggc gtggtcggcg gtcgagtcgc tgctgggcga gtcgctgcag gccgagcgcg
     1081 cgggggatct gctcatcgtt tcgcactaca acacgatccg ctgcctgctc ggccggctgc
     1141 ttgccctgag cgagcaggcc gtgctcgacc tccgcgtgcc caacgccgtg ccgatcgtca
     1201 tccgccacgg ttcgcggtgg gagctggtcg atgggctgga gctgccgtga gagggcagcg
     1261 tgcggcaaag ggtgattcag tgggccgcgc ggctctcggg ccgtcgtgat ttccggtgac
     1321 gagtcgggcc ggatggtaca atgccccgct gggaaactgc cggggtgttt atgcgctact
     1381 cgctcttgcc atgacctatt tcgtcgcctt cagcgctaac tacgacgcat ggccaccaat
     1441 tgaggaaatg ctgcaggtgg aggcggacaa ctcgcaagcc gcagttgagc aagttctgag
     1501 tgacgggaaa gggccaacgg atcatcggtt gcgatgtgct atcgccataa cacttgttaa
     1561 gggccataag tccacgggca gtcgtttttg gataaacttc gatggattgc cagtatgcct
     1621 tagtgatttt ctattctggc catgaccatt tcagtcgcgc cggcagcccc aggcccagca
     1681 agtggacagc agattgtgaa ctgtggtata gtatttgcat aatatcgctc agtttgcgcc
     1741 ggggctgcca gaatggcggc ggtcgcgcgg ggcaggttgg tgaatgagcg ttcgcggatt
     1801 gactgttcag ccaccacttg cgatcagtgc ttgatgccct acaagggtgg ttctcagagg
     1861 aatctgggtc gagtgctaaa ctcctagcct gataggcgag gacttgtgat tacgtgcgtc
     1921 tgtaaagcgt tcgttgacat gattttagat cgaggtttag cgaaggcctt gcagcaaaaa
     1981 gcgggcgaag tttagcgctt ccgcaacgaa gcgcacttgt tggtccactt ctcacccaga
     2041 tctccatcag cggcaatcac caccttgata gggcatgttc aatgaatcga agtcgaactc
     2101 cattcgtcct ggcgattgca aacctgatct tggcgatggt ctttggtcta tgtccgtcgt
     2161 ccggggcgat cgcggcggag cctttgcgga ttgctacctt cgaggcggac attacgccgc
     2221 cgctgggctc gccgctgtgc gatgccttgg tccagccggc gcggacgatc gacgatccgc
     2281 tgctggcgcg agggattgtg ctctttccag agggggagcc gattgtgctc tgtgccctcg
     2341 attgggtcgg catcggcaac agcgggtatg acgcgtttcg cgaggcactt gccaacgcgg
     2401 ccggcacgaa agccgagcgg gtagccgtgc actgcctgca ccagcacgac gccccgggct
     2461 gcgacttcgc cgccgacgac ctcttaaagc cgctcggact aaacaacgaa ctgttcaacg
     2521 cggccttcgc ccgcgaggcg atgcagcgga tcgcgtccgc cgtgagcaaa gcgtgccaaa
     2581 gcccgcagcc ggtgacgcac atcagcctgg gcaaggccaa ggtcgagcag gtggcgtcgg
     2641 cccgccgcgt gatggg
//
LOCUS       DASUGB010000057         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1674604, whole genome shotgun sequence.
ACCESSION   DASUGB010000057 DASUGB010000000
VERSION     DASUGB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674604"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFV87_01075"
     CDS             <1..879
                     /locus_tag="VFV87_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX5102372.1"
                     /translation="VGAQSSDIVLVFKTQKSVENLMRGKFTLGADAAVAAGPVGRRAV
                     AATDGDLKAEIYSYSRSRGLFAGVSIDGSVLEINDAATAAYYGAPGPGGVPQIPDSAL
                     ALVQTVAKLTANPGTIATGEAAAQGVPAAQGVPAAQGVPAAQATPATQGNALVPRQES
                     VLRNAAPTLADPRGQPVLGDPPAAAASETDALRGELSRAAGALNPLLDQGWRRYLALP
                     AEVYQEGRHPATDALQAALTRYDAVARSPQYRTLSERPEFQATHGWLQVYLEKLAAAA
                     GPRTLSLPPPPGTQQR"
     gene            complement(916..989)
                     /locus_tag="VFV87_01080"
     tRNA            complement(916..989)
                     /locus_tag="VFV87_01080"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(953..955),aa:His,seq:gtg)
     gene            complement(1199..2155)
                     /locus_tag="VFV87_01085"
     CDS             complement(1199..2155)
                     /locus_tag="VFV87_01085"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5102373.1"
                     /translation="MRRQIHSVVLFGVAACIAPPTSTAQELPEPDKVQTVELFRVPNY
                     CEGVVFDHEGRGYISWGKSITRFTLDGKNEVWTETGAPNGHKILADGTHLVCDASHHA
                     VLHLAADGKMLDPASKECDGKELRGPNDLTLDTPHGGFYFSDPGGSSLANPIGTLHYV
                     DKEGKTTLLDDGLAFPNGIVLTPDGKKLYLAESQKNRVLVYEVTAPGKVGESMVFAEL
                     PAKEAGTPQIDNQPDGMCLDAAGNLYVAHYGMKQVQVLDPTGKLIRRYEGGNITTSNV
                     AFGGPNMDQLFITGGIGPEAGEGGLFRIDLGVKGLVILPPKK"
BASE COUNT          394 a          792 c          773 g          416 t
ORIGIN      
        1 gtgggagcgc aatcgagcga tattgtgctg gtcttcaaga cgcagaagag cgtcgagaat
       61 ctgatgcggg gcaagttcac gctgggggcc gatgccgcgg tcgccgccgg cccggtcggc
      121 cgccgcgctg tcgccgccac cgacggcgat ctgaaggccg agatctattc ctattcccgc
      181 agccgcgggc tgttcgccgg cgtgtcgatc gacggctcgg tgctggaaat caacgacgcg
      241 gcaacggcag cgtactacgg cgcaccgggc cccggcggcg tcccgcagat tcccgattcc
      301 gcgctggctc tggtgcagac ggttgccaag ctcaccgcca accccgggac aatcgccacg
      361 ggcgaagcgg ccgcgcaggg agttcccgcg gcgcagggag ttcccgcagc gcagggagtt
      421 cccgcagcgc aggctacgcc cgcaacccaa ggcaatgcac tcgttccgcg gcaggagagt
      481 gtcctgcgaa acgccgcgcc cacgttggcc gatccgcgcg gccagccggt gctgggcgat
      541 ccaccggcgg cggctgcgtc ggagacggac gccctccgcg gagaactctc ccgcgctgcg
      601 ggcgcactca acccactgct cgaccaaggc tggcggcgct acctcgcctt gccggcagag
      661 gtgtatcaag aaggccgcca ccccgcgacc gacgccctgc aagcggccct cacccgctac
      721 gacgccgtcg cccgctcgcc gcaataccgc acgctcagcg agcgaccgga atttcaagcc
      781 acgcacggct ggctgcaggt gtatctggag aagctcgccg ccgcagccgg tccccgcacg
      841 ctctcgcttc caccgccccc cggcacgcag cagcggtgaa gcgattccat tcgaacgatc
      901 ccgccggctg tgaggggtgg ctgaggggac ttgaaccccc gacccttaga tccacaatct
      961 aatgctctaa ccagctgagc tacagccacc gtggcgcgcc gcccgcggaa gggcggacac
     1021 gtgaggttga gtttaggtct gccgcgaaat ccggtcaacg gcggacatcg tgggccttcc
     1081 tcgtttaccg cgaagccgaa ggtgcaggcg gatgggcgct ccaacccgca gtcacctgcg
     1141 cccgcgcttc gctgcggcta cggattaaac gacccacggc gggccgagcc ggttcgcact
     1201 atttcttcgg cggcaggatg acgaggccct tgacgccgag gtcgatgcgg aacaggcccc
     1261 cttcgcccgc ttcggggccg atgccgccgg tgatgaagag ctggtccatg ttggggccgc
     1321 caaaggcgac gttgctggtc gtaatgttgc ccccctcgta gcggcggatc aatttgccgg
     1381 tgggatcgag cacctgcacc tgcttcatgc cgtagtgggc aacatacagg ttgccggccg
     1441 cgtcgaggca catgccgtcg ggctggttgt cgatctgcgg cgtgccggct tccttggcgg
     1501 ggagctcggc aaagaccatc gactcgccaa ccttgccggg cgccgtcact tcatatacca
     1561 gcacgcgatt cttctggctc tcggccaggt agagcttttt gccgtcgggc gtgagcacga
     1621 tgccattggg aaacgcgagg ccgtcgtcga gcagcgtcgt cttgccttcc ttatcgacgt
     1681 agtgaagcgt gccgatcggg ttcgcgaggc tgctgccgcc gggatcgctg aaatagaagc
     1741 cgccatgggg cgtatcgagc gtcaaatcgt ttggcccgcg gagttctttg ccgtcgcatt
     1801 ccttggaggc ggggtccagc atcttgccgt ccgccgccag atgcagcacg gcgtgatggc
     1861 tggcgtcgca gaccaggtgc gtgccgtcgg ccagaatctt gtggccgttg ggcgcgccgg
     1921 tctcggtcca gacttcattc ttgccgtcga gcgtgaaccg agtgatcgac ttgccccagg
     1981 agatgtaacc gcggccttcg tggtcgaaaa cgacgccttc gcagtagttg ggcacgcgga
     2041 acagctcaac cgtctgcact ttgtcgggct ccggcaactc ttgagcggtt gaagtcggcg
     2101 gtgcgatgca cgcagcaacg ccaaacagaa cgacgctatg aatctgccga cgcatcgaga
     2161 tttcccctgg aagtcgccca gccacaacgt aaatgccagc ggctaactga ttcttaatca
     2221 aacgcgggcg tttggatacc cccccgaaag gactccctcg cttgcgcttc gggttcgtgt
     2281 gcgattccct cgcgtgcgcg tcgggttggt agtggcgtgc gaaccttcgc tcgcgctgcg
     2341 cggtctatgc cagccgttcg acgcgagttc tcgcc
//
LOCUS       DASUGB010000058         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1118913, whole genome shotgun sequence.
ACCESSION   DASUGB010000058 DASUGB010000000
VERSION     DASUGB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118913"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFV87_01090"
     CDS             <1..668
                     /locus_tag="VFV87_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5102374.1"
                     /translation="LMGHSEGGLVAPAAAVKSDDVAFIVLLAGTGVVGEEILYSQGKL
                     IAKAAGATDEAAAQNEATQRKIFAIVKGTDDPAAAAIKLAALVVEVKASLTDEQKKAV
                     EASAGIAEQQMKMILTPWFRHFLTYDPKPVLEKVKCPVLAICGEKDLQVDPKLNLPPI
                     EAVLKGAGNADFTVKELPGLNHLFQASKGGGIAEYQQIEETINPAALEIVGAWIAKHP
                     LGR"
     gene            670..1164
                     /locus_tag="VFV87_01095"
     CDS             670..1164
                     /locus_tag="VFV87_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5102375.1"
                     /translation="MKQMNDSSGLRITVRSAASDDLPTIVDFNCRLAEETEHKRLELE
                     TITRGVRRALSQPELCRYFVAEWDGAIVGQAMITVELTDWRDGVLWWLQSVYVAEPHR
                     GQGIFRALFERIRSLAEADPDVRGIRLYVERDNVRAQRVYEQMGLAPSGHLVYERDWS
                     GLAK"
     gene            complement(1173..1769)
                     /locus_tag="VFV87_01100"
     CDS             complement(1173..1769)
                     /locus_tag="VFV87_01100"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5102376.1"
                     /translation="MTDDQLFRFILLAGFLITLPIAIYHRVRAHTGEPLDRRQEGWLL
                     LIAIRVLGGVGMLAALALAFASPSWMTWSIVPLPTWIRWLGVGLGCFATGLLIWTFRT
                     LGKNITDTVVTRREHTLVTSGPYRWVRHPFYVAFLLGMTAIAIVLANWLLLMIGIAAF
                     TLLAIRSRKEEANLELRFGDNYRRYREQTEAFYPRLFG"
     gene            complement(1807..3207)
                     /locus_tag="VFV87_01105"
     CDS             complement(1807..3207)
                     /locus_tag="VFV87_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5102377.1"
                     /translation="MKSIHWMGVAIVLALAGLARGQDQVPWAGDFQQACQQAAQQHKL
                     VLLHFYSDDCPPCVRVEQNVFANPEAAQAIARNYVPLKVHVKQNPQLAARYQITRWPT
                     DVAVTPVGLEVHRSTSPQSAEHYIELVDLIALKGGVGAGRQWALDMQQVGQATLAQAG
                     AAAAGAAGEARQQWDQTAQQFQGAADQARGAATQWGQQAQAATQQYSQQAQDAIAQYQ
                     QQAQGTFQQFNQQAQGAAQQFQESTQQFVDAARPSATQWKPYADPAELRGAGPSAGAG
                     AFAAAAGQAAANVGSPPQTGIVNPFFGAPSPAAPGGAPQQTPQSFQAEFQPQQEAATQ
                     YVAASQAPPVAMEGYCPVTLIEMRKWMKADPQFGAIHRGRTYLFAGAEQQQKFLANPD
                     AFAPVLSGYDPVRFATTGQLVDGKRGHGIFNPEDKRIYLFADEAALEQFKQSPRAYAE
                     PAYQAMMRSESATTYR"
BASE COUNT          671 a         1166 c         1168 g          645 t
ORIGIN      
        1 gcctgatggg gcacagcgaa ggaggcctcg tcgcacccgc ggctgcggtg aaatcggacg
       61 acgtggcctt catcgtgctg ctcgccggca cgggagtggt gggcgaggag attctctact
      121 cccagggcaa gctgatcgcc aaggccgccg gcgccacgga cgaggcagcg gctcagaacg
      181 aagcgacgca aaggaagatc ttcgcgatcg tgaagggtac ggatgatcca gcggccgctg
      241 cgatcaaatt ggccgcgctc gtcgtggaag tcaaagccag cctgaccgac gaacagaaga
      301 aggcggtgga agcctccgcg ggcatcgccg agcagcaaat gaagatgatc ctcacgccct
      361 ggttccgcca cttcctcacc tacgatccca agccggtcct ggagaaggtg aaatgcccgg
      421 tcctcgccat ctgcggcgag aaggacttgc aggtcgatcc gaagctcaac ctgccgccga
      481 tcgaagccgt cctcaagggc gccggcaatg cggacttcac ggtcaaggaa ttgcccggcc
      541 tcaatcatct gtttcaagcc agcaaaggcg gcggcatcgc tgaatatcag cagatcgagg
      601 agacgatcaa tccagcggcg ctagaaatcg tcggcgcttg gattgcgaaa catccacttg
      661 gaaggtgaaa tgaaacagat gaatgattca tctggattgc gcatcaccgt ccgttctgcc
      721 gcctctgacg acctgcccac cattgtcgat ttcaattgcc ggctcgccga agagaccgag
      781 cacaaacggc tggagttgga gacgatcacg cgcggcgtgc ggcgggcgct gtcgcagccg
      841 gagctgtgcc ggtacttcgt cgcggagtgg gatggggcga tcgtgggcca ggcgatgatc
      901 acggtcgagc tgaccgattg gcgcgatggc gtgctgtggt ggctgcagag cgtctatgtc
      961 gccgagccgc accgcggtca gggcatcttc cgggcgttgt tcgagcgcat ccgctcgctg
     1021 gccgaggccg atccggacgt gcgcggcatc cggctgtatg tcgagcgcga caacgtgcgg
     1081 gcccagcgcg tctatgagca gatgggcctg gcgccgagcg ggcacctggt ctatgagcgc
     1141 gactggtcgg gcttagcgaa gtaagaagcg agctatccga acagacgcgg atagaatgcc
     1201 tcggtttgtt cacggtagcg gcggtagttg tcgccaaacc gtagttccag gttcgcttct
     1261 tccttgcggc tgcggatggc gaggagcgtg aaggcggcga tgccgatcat cagcagaagc
     1321 cagttcgcca gcacgatcgc aatcgccgtc atgcccagca ggaacgcaac gtagaacgga
     1381 tgccgcaccc agcgataggg cccgctggtg acgagcgtgt gctcgcggcg cgtgacaacc
     1441 gtgtccgtga tgttcttgcc cagcgtccga aaggtccaga tcagcagtcc ggtcgcgaaa
     1501 catcccaggc cgacgccgag ccagcggatc cacgtcggca gtggcacgat tgaccaggtc
     1561 atccagctcg ggctggcgaa ggcgagggcc agtgcagcca gcatccccac tccgcccaac
     1621 acgcggattg caatcaacag tagccagcct tcctggcggc gatcgagcgg ctcacccgtg
     1681 tgggcccgca cgcggtgata gatcgcaatg ggcaacgtga tgaggaatcc agccagcaga
     1741 atgaagcgga acagctgatc gtcggtcatc gtccgacatc ctacctgaac cacctcgcgc
     1801 gggcgcttac cgatacgtag tcgccgactc gctgcgcatc atggcctgat aggcgggctc
     1861 ggcataggcc cgcggcgact gcttgaactg ctccagcgcc gcttcgtcgg caaagaggta
     1921 aatgcgcttg tcttcggggt tgaagatgcc gtggccgcgt ttgccatcga cgagctgtcc
     1981 cgtcgtggca aaccgcaccg gatcgtagcc cgacagaacc ggggcgaagg catccggatt
     2041 ggccaggaat ttctgctgct gctcggcgcc ggcgaacaga tacgtgcggc cgcggtggat
     2101 cgcgccgaac tgcggatcgg ccttcatcca cttcctcatt tcgatcagcg tcaccgggca
     2161 atagccttcc atggcgaccg gcggcgcctg cgaagcggcg acgtactggg tggcagcttc
     2221 ctgctgcggc tgaaactcag cctggaacga ctgcggagtc tgttgcgggg cgccaccagg
     2281 agcagcagga gacggcgcgc caaaaaacgg atttacaatt cccgtttgcg gcgggctgcc
     2341 gacattcgct gcggcctgcc ccgcggcagc ggcgaaagca cccgcgccgg cggagggacc
     2401 ggccccgcgc agctcggccg gatcggcata cggcttccac tgcgtggcgc tcggccgggc
     2461 cgcgtcaaca aattgctgcg tggattcctg gaattgctgc gctgcgccct gggcttgctg
     2521 attgaattgc tggaacgtgc cttgggcctg ctgctggtac tgggcgatcg cgtcctgcgc
     2581 ctgctggctg tattgctgcg tggcagcctg cgcctgctga ccccactggg tcgcggcccc
     2641 gcgggcctga tccgcagcgc cctggaactg ctgcgctgtc tggtcccact gctggcgagc
     2701 ctcgccggca gcgcccgccg ctgcggcgcc cgcttgggcc agtgtcgcct ggccgacttg
     2761 ctgcatgtcc agcgcccatt gtcgcccggc gccgacgccg cctttgagcg cgatcaaatc
     2821 gaccagctcg atatagtgct ccgccgattg cgggctggtc gagcggtgta cttccaagcc
     2881 gacgggcgtg accgccacgt cggttggcca gcgcgtaatc tgatagcgag ccgccaattg
     2941 cggattctgc ttcacatgca ccttgagcgg cacataattg cgggcgatcg cctgggccgc
     3001 ttccgggttc gcaaagacat tctgctcgac gcgcacgcag ggggggcaat cgtcggaata
     3061 aaagtgcagc agcaccagct tgtgctgctg ggccgcttgc tggcaggcct gctgaaaatc
     3121 gccagcccat gggacctggt cctgaccccg agccaggccg gccagtgcca gcacgatcgc
     3181 gacgcccatc cagtggatcg acttcatttc cgcacattgc tccccgcaag cgtaattcag
     3241 ctctcacgcc atcacggcat tcgcgcagcc ggcctccaag gatcgtctgc ctccaggatg
     3301 accgcgcagc cccatccgcc tgatggtgcg tatggacgaa gatcggctag ttggcccctc
     3361 cgattacagc aaatctgccg attgtaggga caggactcat ttcacggatc gagggcgagt
     3421 caggcgcgcg atgcgcaact tttccagttt tttcacaccg ctcctccccg cgcaattatc
     3481 tcttaactcc agtacttgca accacttgcg ccgcgcaaca ccccccagat gaggatgttg
     3541 cgctgttgcg cgttgtcacc tcgaaaatct cgccgcggcg ccctgccgcc gcctgcgcgg
     3601 cggtgggcac agctcaccct accaactaca aacgtgacgc ccgccctcgt
//
LOCUS       DASUGB010000059         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1953174, whole genome shotgun sequence.
ACCESSION   DASUGB010000059 DASUGB010000000
VERSION     DASUGB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953174"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFV87_01110"
     CDS             complement(<1..525)
                     /locus_tag="VFV87_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102378.1"
                     /translation="MEPGHKRLIAAFIAALMIAHGVAIAARIDQWPLSNYAMFAQRKE
                     STVTWLVLMGVTECGEEFRLDDPSYWHPYSSAKLAHCLRDAKRQDSRRQETQRPAIQR
                     QGATLSSAGQPKPPVLPAAVKSLLEHYESRRQAGLHAGPPLAGLRLYECQWHLDPRLA
                     NVNRPDRQELIYEFP"
     gene            911..2374
                     /locus_tag="VFV87_01115"
     CDS             911..2374
                     /locus_tag="VFV87_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5102379.1"
                     /translation="MSFTTLVALAAIATSGDAVLVQFASEACRPCQAMQPAVSRLVSD
                     GYPVQRIDVDRFPETARQFGVKAVPTFVLLVGNQEAGRIEGPTSYDRLAAMFTAAGPR
                     QAVATVEPANPPAAAVQPTDFRSQFGGSASCQQAALAASVRLKVEDAAGYGFGTGTII
                     DTHGDEALIVTCGHLFRESQGKGRISVDLFAPGGPQEVPGELLACDLNRDIALVGIKP
                     GVEVTPAAVAPAGFVVQPQDRVFSVGCDKGADATVRDSQITAVNKYHGKPNFTAKGAP
                     IDGRSGGGLFTAQGLLIGICNAADPQDDEGLYAGLASIHWQLDQIGQTEIYQRQAGHI
                     AAAAAGRGPGATADAGIALASAELPLAHALPAAPPSSPAGMPSAGLPPGWTAGQPAQL
                     GESGRSPVAGSLDDDTELVVIIRSKRNPQTRSEIYVVDQASPELLTGIVHAARQGAQQ
                     RTAALAARQPSAAMAPLPPDSPFPRGSQPVVRAQSAE"
     gene            2495..2671
                     /locus_tag="VFV87_01120"
     CDS             2495..2671
                     /locus_tag="VFV87_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102380.1"
                     /translation="MSFFLLLLITVGNLALGFGLAVHLGFGPDFTRLAAYWQELRSST
                     GLKKPDDKSMESGN"
     gene            2868..>3269
                     /locus_tag="VFV87_01125"
     CDS             2868..>3269
                     /locus_tag="VFV87_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102381.1"
                     /translation="MSTKSMRRSLAFESLETRRVLTAGGSHAAAMSLVDGVLTVEGTH
                     KNDQITVCLAGDMGQQLSVRVGKSAQLFELVDVTELHINGNNGNDRIRVGDGVTVSAV
                     IDGGKGNDWLWGGGGADVVHGGAGNDHIYGGG"
BASE COUNT          564 a         1058 c         1060 g          587 t
ORIGIN      
        1 tgggaattca tatatcagct cctgccggtc ggggcgattc acgttggcca ggcgcggatc
       61 caagtgccat tggcattcgt acagtcgcaa tccggcgaga ggcggccctg cgtgcaatcc
      121 agcctggcgg cgggattcat aatgctccag cagcgacttg accgccgccg gcaataccgg
      181 cggtttaggt tggccggcgc tggaaagggt ggcaccttgc cgctggatcg ctgggcgctg
      241 agtctcttgc cgccgggaat cctgtcgctt cgcatcgcgc aggcaatgcg ccagcttggc
      301 gctggaataa ggatgccaat agcttggatc gtcgagacga aattcctcgc cgcactccgt
      361 aacgcccatc agaacgagcc aagtgacggt gctctctttc cgctgggcga acatggcata
      421 gttcgacagc ggccactggt cgattcgcgc ggcgatcgct acgccatggg cgatcatcag
      481 cgcagcgatg aacgcagcga tcaagcgttt gtggccgggt tccatgcggc ggaaggataa
      541 agtcgctcgc gtccaattcg caagagcaac gctcggcatg ccgtatgctc accgcgtccc
      601 gccggcgcat cgacagcggt ggcgcggccg gtttgcggcc acccgttgcc gccgcgcggc
      661 caattggcct tgtaagaatt tcaattccgg accagattgg gccctggcgt ggcgagtagt
      721 ccagccgggg ataggcaatt tgttcccgct ggtttgcgag cgcacgctcc cgaaggaccg
      781 ggaaaacgac tcgagaacaa agattcggaa cgttccattc cgagaagtcg gggcaaggaa
      841 gctccccgcg gccaatcggc cacgcttccc tgcccccgcg actcgccagg cagttccaag
      901 gagggaagcc atgtctttta ccacgctcgt ggcgttggcc gcgattgcta ccagcggcga
      961 tgcggtgctc gtgcagttcg cttccgaagc gtgccggccg tgccaggcca tgcagccggc
     1021 ggtctcgcgc ctggtcagcg atggatatcc agtgcagcgt atcgacgtcg atcgcttccc
     1081 cgagacggcc cggcagttcg gtgtcaaagc ggtgcccacg ttcgttctgc tcgtgggcaa
     1141 tcaagaggcg ggccgcatcg aaggacccac cagctacgac cggctggcgg ccatgtttac
     1201 ggccgcgggg ccgcggcagg cagtagcgac tgttgaaccg gcgaacccgc ctgcagccgc
     1261 ggtccagccg acggacttcc gctcacagtt cggcggcagc gcgtcttgcc agcaggcggc
     1321 cctggccgcc agcgtacgcc tgaaagtcga ggacgccgcg ggctacggct ttggcacggg
     1381 cacgatcatc gacacgcacg gcgacgaagc gctaatcgtc acgtgcgggc acttgtttcg
     1441 cgagtcgcag gggaagggcc ggatttccgt cgatctgttt gcacccggcg ggccgcagga
     1501 agtgcccggc gagctgctgg cttgcgattt gaatcgcgac atcgctctgg tcggcattaa
     1561 gccgggcgta gaagtcacgc cggcagccgt cgctccggcg ggatttgtcg tccagccgca
     1621 agaccgcgtg ttcagtgtcg gctgtgacaa gggggcggac gcaacggtga gggacagtca
     1681 gatcacggcc gtcaacaagt atcacggcaa gccgaacttc acggccaagg gcgcgccgat
     1741 cgacggccgc agcggcggcg gattgtttac ggcacaggga ctgctcatcg ggatttgcaa
     1801 cgccgccgat ccgcaagacg acgaagggct gtacgccggc ctggcatcga tccactggca
     1861 actcgatcag atcggtcaga cggagattta tcagcggcag gcggggcaca ttgccgcggc
     1921 ggctgccggt cgcggtcccg gtgcgacggc cgatgcaggc attgccctgg cttcagctga
     1981 gttgccgctg gctcacgcgc tgccggccgc gcccccctca tcgcccgccg gcatgccgtc
     2041 agccggcttg ccacctggct ggacggctgg ccagcccgcg cagctcggcg agtccggtcg
     2101 ctccccggtt gccggctcgc tcgacgacga caccgagctg gtcgtcatta tccgctccaa
     2161 gcgcaatccc cagacccgca gcgagattta cgtcgtcgat caggcgtcgc cggagctgtt
     2221 gaccggcatc gtccatgccg ccaggcaagg cgcccagcag cgcacggccg cgctggccgc
     2281 ccgccagccc tcggctgcca tggcccccct accgccggac agtccgttcc ctcggggcag
     2341 ccagccggtc gttcgtgcgc aatcagccga atgatctaat cgctccaatc gaaaaacgcc
     2401 gcacgaccgg cagcacccgc ggtttgctgc ccgcgctgcg ctcgctgact gacctagagt
     2461 tgcccggccc ttcccccgcg agcgagcact tgccgtgtcg tttttcctgc tcctcttaat
     2521 taccgtcggc aacctggcgc tcggattcgg tctggcggtc caccttggtt ttgggcccga
     2581 tttcacccgc ctggcggctt attggcagga actgcggagc tcaactggtc tcaaaaagcc
     2641 cgacgataag agtatggaat ctggcaatta agggccggcc tgtctgttct gcccattagg
     2701 ctcaaattgc aagccgttgc tgctcaatag gttgcgcatt tcttcccctc gcattacggt
     2761 gctccgcacc ccgcgttttg acaatccgca cccgtgacct agggtttctg tgagccattc
     2821 ggctccgctc tatcgcgagc ttcacctagg cttatcggag tcaggtcatg tcaacgaaat
     2881 ccatgcgccg cagtctggcg tttgagagtc tggaaacgcg ccgagttctg accgccgggg
     2941 gctcgcacgc tgccgccatg tcgctggtcg atggcgtgct gacggtcgag ggcacgcaca
     3001 agaacgacca gatcaccgtc tgcctggccg gcgacatggg gcagcagctc agcgtgcgcg
     3061 tcggcaaaag cgcgcagctg tttgagctgg tcgatgtgac ggaattgcac atcaacggca
     3121 acaacggcaa cgatcgcatc cgcgtgggcg atggggtcac cgtgagcgcc gtcatcgacg
     3181 gcggcaaggg gaacgactgg ctgtggggcg gcggcggagc cgacgtggtc cacggcggcg
     3241 ccggaaacga ccatatttat ggcggcggc
//
LOCUS       DASUGB010000060         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1258039, whole genome shotgun sequence.
ACCESSION   DASUGB010000060 DASUGB010000000
VERSION     DASUGB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258039"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFV87_01130"
     CDS             <1..837
                     /locus_tag="VFV87_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5102382.1"
                     /translation="VRDHIRTYCHLGGGKMEDFYETRADDAARFADLARQAVADGFTA
                     FKSMAVPPTLPIEGLAPIRAAEAAVAAMREAVGDQIDIMVDCHARPSPAMGLQFAKAL
                     EPYGLYFLEEPCWPECASGLAEINAAVSTPIATGERVTNLAAFRDLFNARACDVCQLD
                     ITHCAGLSEARRIAALADAHRIALAPHNPQGPVSTAASLEFGFSQPSYIICETVHADV
                     PWRQDVVQEGFTAERQGRIVKPNTKSGLGITINETEVKKHPFQQEVLQRVFYRDGSVG
                     DW"
     gene            1013..1945
                     /locus_tag="VFV87_01135"
     CDS             1013..1945
                     /locus_tag="VFV87_01135"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5102383.1"
                     /translation="MPKPLHGVLPIVQTPFLDNEDIDWATLQREVDWAFAQGIDGLGT
                     GMVSELLKLTADERALLALKLVEFAAGRGPVFMGVGAESTRLAVEYAIAAERAGCDAV
                     MATPPMTTRSNEAGLVAYFRGLADSVSLPVIVQDASGYVGQAIPLKVYADLLDRYGPE
                     KILFKPEASPVGPLLSAVRDMSGSKARIFEGSGGIFLIDSFRRGIAGTMPAVEMVDAI
                     VALWQAIETGDEELAYRISFPIGAIVALQLQADLDGFLAIEKHLLVKRGLFPSARRRG
                     PYNWELDAETAAEVDRLFDQLQVILAESGEAASE"
     gene            2021..3463
                     /locus_tag="VFV87_01140"
     CDS             2021..3463
                     /locus_tag="VFV87_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102384.1"
                     /translation="MTTIETPQTRCLAGIARADITPPVGIYHRMWGAATHDRATGVHK
                     PLLATLLWLEPAGGDERRSLCIASLDHCILDGPDIELMERSIADAVAMRPGQTLITLS
                     HTHAAGLMSRSRADQPGGELIGPYLDSLATRLGQLAVEAIDHRQPATIVYGRGKCGLA
                     ANRDFFDPQAQKFVCGLNPGGIADDTLLVGRISGADNRVIGTLVNYACHPTTLAWQNT
                     LISPDYIGALRETVESGFHAPCLFLQGASGDLGPREGYVDDTGVADRNGRQVGFAALA
                     ALATLPPAGSQFVYAGPVVSGATLGTWRHEPVDARAADRQKQWHSDTFDLPLDYRPEL
                     ATKEQTQADLARWQAEEQKATAAGDSLSARDAHAQVERMTRQLWRLKSLPAGQFPLRI
                     TIAQLGDALWVFVPGEHYQNLQTALRQRFPQHPVIVATLTAGWQPGYIPTADTYGRGI
                     YQSDIAVVAAGSAERIIDEVGRRIAELIAS"
BASE COUNT          639 a         1183 c         1139 g          623 t
ORIGIN      
        1 gttcgcgatc atatccgaac ctactgccac ttgggcggcg gcaagatgga ggatttttac
       61 gaaacgcgag ccgatgacgc cgctcgcttc gccgaccttg cccggcaggc ggtcgcggac
      121 ggcttcacgg cgttcaagtc gatggctgtg ccgccaacgc tgccgatcga gggtctcgcc
      181 ccgatccgcg ccgccgaagc ggccgtggcc gccatgcgcg aagcggtcgg tgaccagatc
      241 gacatcatgg tcgattgcca cgcccggcct tcgcccgcga tggggctgca gttcgccaag
      301 gccctggagc cgtatggact ctatttcctg gaagaacctt gctggccgga gtgcgccagc
      361 ggactggccg agatcaacgc cgcggtcagc acgccgatcg ccaccggcga gcgggtcaca
      421 aacctcgccg cgttccgcga cttgttcaac gcccgggcgt gcgacgtctg ccagctcgac
      481 atcactcact gcgccggctt gtccgaagcc cgccgcatcg ccgcgttagc cgacgcccac
      541 cggatcgctc tggcgccgca caacccgcaa ggccccgtca gcaccgccgc ctcgctggag
      601 ttcggcttct cgcagccgag ctacatcatt tgcgaaacgg tccacgccga cgtcccctgg
      661 cggcaggatg tcgtgcaaga aggcttcacc gctgaacgcc aaggacggat cgtaaaaccc
      721 aacacgaaat ccggcctcgg catcacgatc aacgaaaccg aagtgaagaa gcacccgttc
      781 cagcaggagg tcctgcagcg ggtcttctat cgcgacggca gcgtggggga ttggtagcag
      841 gcgatcttgc cgcggatcaa cgcggatgac gcagatgggg aggagggatt ggccccgggt
      901 cccgggttct gcctggggcg gagaatctcg tcctctttgc gtccttcgtg cccttttgtt
      961 ccaatctccc tgtccgatcc gcgttcatcc gcgtcatccg cggccaagca aaatgccgaa
     1021 gcccctccac ggcgtcctcc cgatcgtgca gacgcccttt ctcgacaatg aagacatcga
     1081 ctgggcgaca cttcagcgcg aagtcgattg ggcgttcgcg caaggaatcg acggcctggg
     1141 gaccgggatg gtttcggagc tgctcaagct gacggcggat gaacgagcgc tgttggcact
     1201 caagttggtg gagtttgcag cgggacgcgg gccggtgttc atgggggtcg gcgcagagag
     1261 cacgcggctg gcggtggagt acgcgatagc agccgagcgg gcaggctgcg acgccgtgat
     1321 ggccacgccg ccgatgacga ccaggtcaaa cgaagcgggc cttgtcgcct atttccgcgg
     1381 cctggcagat agcgtcagcc tgccggtgat tgttcaggac gccagcggct atgtcggtca
     1441 ggcgatcccg ctcaaggttt atgccgacct gctggaccgc tatggcccgg agaaaatcct
     1501 cttcaagccg gaagcctcac ccgtcgggcc gctgctttcc gcggtgcgcg acatgagcgg
     1561 cagcaaagcc agaatcttcg agggttccgg cggcatcttc ctgattgaca gcttccgccg
     1621 cgggatcgcc gggacgatgc cggcggtcga aatggtcgat gccattgtcg cgctctggca
     1681 agcgatagaa accggcgacg aagagctcgc ctaccgaatt tcgtttccga tcggcgcgat
     1741 tgtcgcgctg caacttcaag ccgacctcga cggcttcctg gcgatcgaaa aacacctgct
     1801 cgtcaagcgc ggcctgttcc cctccgcccg ccgccgcggg ccgtacaact gggaactcga
     1861 cgccgaaacg gctgccgagg tcgatcggct gttcgatcag ttgcaggtca tccttgccga
     1921 atcgggtgaa gcagcgagtg agtgaatacc aacccgaagc gtaagcgagg gagtcttccg
     1981 gtggtcctac tgcactccct cgcttacgct gcgggttact atgacgacga ttgaaacacc
     2041 acaaacccgc tgcctcgccg ggattgcccg ggccgatatt acgccgccgg tcggcatcta
     2101 tcaccgcatg tggggcgcgg cgacgcatga ccgggcgacg ggcgtgcaca agccgctctt
     2161 ggcgacgctg ctgtggctgg agccggcagg cggcgatgag aggcggtcgc tgtgcatcgc
     2221 gtcgctcgac cactgcattc tggatggtcc agatattgag ctgatggagc ggtcgatcgc
     2281 cgacgcggtg gcgatgcggc cgggccagac gctgatcacg ctctcgcaca cgcacgccgc
     2341 cggcctcatg tcgcgctctc gcgccgatca gccgggtggg gagttaattg ggccgtatct
     2401 cgacagcctc gccacccggc tgggacaatt ggcagtggaa gcgatcgacc atcgccagcc
     2461 agcgaccatt gtttatgggc gcgggaagtg cggtctggcc gcgaaccgcg actttttcga
     2521 tccgcaggcg cagaagttcg tgtgcggcct caatccgggc gggattgccg acgacacgct
     2581 cctcgtgggc cgaatttcgg gcgcagacaa ccgcgtgatc ggcacgctgg tcaactacgc
     2641 ctgccatccg acgacactgg cctggcagaa cacgctgatc agccccgact atatcggcgc
     2701 actccgggaa acggtcgaat ccgggttcca cgctccgtgt ctgttcctgc aaggggccag
     2761 cggcgacctc ggtccgcggg agggttatgt cgacgacaca ggcgttgccg atcgcaatgg
     2821 caggcaggtc ggctttgcgg cgctggcggc tttggcaacg ctgccgcccg ctggatcgca
     2881 gttcgtctat gccggcccgg tcgtcagcgg cgcgacgctc ggcacctggc ggcacgagcc
     2941 ggtcgatgcc cgcgccgctg atcgccagaa gcagtggcac tcagacacat tcgatctgcc
     3001 gctcgactat cgccccgagc tggcaacgaa agagcaaacg caagccgacc tggcccgttg
     3061 gcaagcagag gaacaaaagg caactgcggc tggcgattcg ctctcggccc gcgatgccca
     3121 cgcccaagtc gaacgaatga cccgccagct ctggcggctc aaatcgctgc cggcggggca
     3181 gttcccgctg cggatcacaa tcgcgcagct cggcgatgcc ctgtgggtct tcgttcccgg
     3241 cgagcactac cagaacctgc aaacagccct ccgccagcgg tttccccagc acccagtcat
     3301 cgtcgccacg ctcactgccg gctggcagcc tggctacatt cccacggccg atacttacgg
     3361 ccgcggcatc tatcaatcgg acattgccgt ggtcgccgcc ggcagcgcgg agcggatcat
     3421 cgacgaagtc ggccggcgaa ttgccgaact gattgcctcg tagccccgtg ctgcaattag
     3481 aatgcgccca tattgtagcg ggggcttcca gcccccgacg cgatggctag aagccaccgc
     3541 tacttccttg gagcctgcac catgcgcaat tcgaatcgcc tcct
//
LOCUS       DASUGB010000061         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1118985, whole genome shotgun sequence.
ACCESSION   DASUGB010000061 DASUGB010000000
VERSION     DASUGB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118985"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFV87_01145"
     CDS             <1..428
                     /locus_tag="VFV87_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5102385.1"
                     /translation="FQLPARFKADQKRLESFLKMLSRRRIYAFEFRDESWYDEPIFAL
                     LRKHNVALCLSDHRDAPAPWEVTAGHVYVRGHGPTGQYKDRYPRKTLQSWASDMARWR
                     RKGLATYCYFDNDQKSAAPKDARRLAELVGGSALARRAG"
     gene            complement(566..1570)
                     /locus_tag="VFV87_01150"
     CDS             complement(566..1570)
                     /locus_tag="VFV87_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5102386.1"
                     /translation="MVRSLLAAGFLLAATAGPAAADPPPERLVVATWNLEWFYDDNTG
                     DNYADLAKEQSAPSRDEWDWKLAGVAQTIGQIRPTILALQEVENQRVLFYLTRKLKQE
                     YGLDYATAYIEGGDFFTEQDVAVLALSGLTGFSVRRQSREMAESKQFYNVTKHLLCQF
                     EWGSGAQRERLLLCNVHFRAQPMGAAIRVKQAHLVRRWIDEAVLAGENVMVVGDVNTE
                     ETFATTTKDGDLGALRGLDTPAAGDDLADLLAHLHGQSHETHLVHKQFDHILVTPSLV
                     ADAPDKSDLVFKSIAIRKGLVVQGKEQDQDHRDVFWQIPSAERDISDHYPVVAEFEFR
                     "
     gene            1783..>2318
                     /locus_tag="VFV87_01155"
     CDS             1783..>2318
                     /locus_tag="VFV87_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX5102387.1"
                     /translation="MPSITLAEIIAEFEELDDPADRLAYLVEIGKSLPPLDDAFRTEE
                     HRVLGCQSMVWMVADERKGTPPTLSFTADSDAPMVRGLIAILLAAYSNHTPAEILAFP
                     IEQLFERLKLLSFLTPMRSNGLFSMVKRIQNLARRAQAAGPQAGAPGSPLVQVRMQPR
                     SELLPAAPRRTSPARPGL"
BASE COUNT          409 a          773 c          728 g          408 t
ORIGIN      
        1 tgtttcaatt gcctgcccgt ttcaaggccg accagaagcg cctggaatcg ttcctcaaaa
       61 tgctctcccg gcggcggatc tatgcgtttg agttccgcga cgagagctgg tacgacgagc
      121 cgatcttcgc cctgttgcgc aagcacaacg tcgcgctctg cctgtccgac catcgcgacg
      181 ccccggcccc gtgggaagtg acggcggggc acgtctatgt tcggggccac gggccgaccg
      241 gccagtacaa ggaccgctac ccgcgcaaga cgctccagtc gtgggccagc gacatggccc
      301 gctggcggcg caaagggctg gcgacctatt gctacttcga caacgaccaa aagagcgccg
      361 cgccgaaaga cgcccgccgg ctcgcggagc tggttggagg ttccgctttg gcccgacgag
      421 ccgggtgaac ggagacgggg agatagagag acagagagac agagagacag agagacagag
      481 agacggagag acggagagac gccagaatca gcgaccactg acctccgatc tctacgctct
      541 acgctctgcc ctccgaccta tcgctttagc ggaactcgaa ctcggccacc accgggtagt
      601 ggtcgctgat gtcccgttcg gctgagggaa tttgccagaa cacgtcccgg tggtcctggt
      661 cctgctcctt gccttggacg accaggccct tgcgaatcgc gatcgacttg aagaccaggt
      721 cgcttttgtc gggcgcatcg gccaccagcg acggcgttac gagaatatga tcgaattgct
      781 tgtgtaccag gtgcgtctcg tgcgactggc cgtgcaggtg agccagcaag tcggcgaggt
      841 catcgccggc tgctggcgta tccaggcccc gcaaggcgcc cagatcgccg tccttggtgg
      901 tcgtcgcgaa agtctcctcg gtgttgacgt ctcccaccac catcacattc tcgccggcga
      961 gcaccgcttc gtcgatccag cggcgcacca ggtgcgcctg cttgacgcgg attgccgccc
     1021 ccatcggctg ggcccgaaaa tggacgttgc acaacagcag ccgctcgcgc tgggcgccgc
     1081 ttccccactc gaactggcac agcaggtgct tggtgacgtt gtaaaactgt ttcgattcgg
     1141 ccatttcccg cgattgccgc cggaccgaga agccggtgag tcctgacagg gcgagcacgg
     1201 ccacgtcctg ctcggtgaag aagtcgcccc cctcgatgta ggccgtggca tagtccaggc
     1261 cgtattcctg cttgagcttg cgggtcagat agaacagcac ccgctggttt tcgacctcct
     1321 gcagggcgag gatcgtcggg cggatctggc cgatcgtctg ggccacgccg gccagcttcc
     1381 agtcccactc gtcccgcgag ggggccgact gctccttggc caggtcggca tagttgtcgc
     1441 cggtgttgtc gtcgtagaac cactccaggt tccacgtagc caccacgagt ctttccggcg
     1501 gcggatcggc ggcggccggg ccggcagtcg cagcgagcag aaaaccagcg gccaaaaggc
     1561 tgcgcaccat gaaaaccgtc ccctcgcgca aaactgcgtt ccgttgccga tgaattccgc
     1621 tgcggcggcg gccacacgcc gcgattcgcc tcgccattgc ccagacgagc cggggtgctg
     1681 ggcttctgta tcgagcgtct ataataacca agaaggcgcc tttcctgccg ttcccgcccc
     1741 catcagcggc tgcaaccgca accgcagcga gagccatttc ggatgccgag catcacgctg
     1801 gccgaaatca ttgccgaatt cgaggagctg gacgatccgg ccgatcgcct ggcttatctc
     1861 gtcgagattg gcaagtcgct gccgccgctg gacgatgcgt ttcggaccga agagcaccgc
     1921 gtcctcggct gccagagcat ggtttggatg gtggccgacg aacgcaaggg cacgccgccg
     1981 actttgtcct tcaccgccga cagcgacgcc ccgatggtcc gcggcctgat cgccattttg
     2041 ctggcggcct attccaatca cacgcccgcc gaaatcctgg cctttccgat cgagcagctc
     2101 ttcgagcggc tcaagctgct ctcgttcctg acgccgatgc gcagcaacgg cctgttttcg
     2161 atggtgaagc ggatccagaa cctggcccgc cgggcccagg ccgccggtcc gcaagccggt
     2221 gcgcccggca gcccgctcgt gcaggtgcgg atgcagccgc ggagcgaatt gctgccggcc
     2281 gcgccgcggc gcaccagccc ggccaggccc gggctgat
//
LOCUS       DASUGB010000062         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_841576, whole genome shotgun sequence.
ACCESSION   DASUGB010000062 DASUGB010000000
VERSION     DASUGB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841576"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFV87_01160"
     CDS             complement(<1..557)
                     /locus_tag="VFV87_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102388.1"
                     /translation="MVIPQRAIVRPRVFGPEGTVALPVEEVVAELLYRGGDRPIQIMG
                     GPGSGKTTASRHLAATLFPVSRLVFLDDTTEAKFKESGYRVILASRKPVERDCDVLRL
                     APWTDDDLIEYLLATCPSRCGSVMQRLRDSTDKALIQGNPAVWRLTLDALAADAELPD
                     IRAAIDRWLPVMFRNPEERRLAGLW"
     gene            complement(674..1009)
                     /locus_tag="VFV87_01165"
     CDS             complement(674..1009)
                     /locus_tag="VFV87_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102389.1"
                     /translation="MARKVVNRKELRAEAEAAEAQAKAAGTEVGPDGKKVKAAKEKKP
                     PAKRKSRSKTPEVIRMKLFWGVFNQSLKRVALFDFTQEKAAKAKAAELSASGKSPHFV
                     QKVKEAIQE"
     gene            complement(1123..1770)
                     /locus_tag="VFV87_01170"
     CDS             complement(1123..1770)
                     /locus_tag="VFV87_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102390.1"
                     /translation="MDNNGLQSLLQFAPPPLRPPAVHPPNDKSWRAVETHFGFELPPD
                     YKLLIEHYGTGCWGGFLQIFNPFSRNRYNNQRLIIENSHLLCVEEPDPPSSHPEFLRA
                     WNLFRSAARARRAASPYDFYPARPGLFPWAQGTDEAVTLYWLVSGEPSSWQTIVERWS
                     DGPTFDQRFEVGSSAFLLSWLQNSIRPAAFPEAARSAKGWFEPWILHDVELTDLG"
     gene            complement(1832..2128)
                     /locus_tag="VFV87_01175"
     CDS             complement(1832..2128)
                     /locus_tag="VFV87_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5102391.1"
                     /translation="MHPDDELCLCFHVTKRKVVNWIRIEKPKRPAQLSECFGAGTGCG
                     WCRPFLERLFEQAVEGGQTAVDFPTPEEYAQMRAKYVREGGGTPPPGATPLPEE"
BASE COUNT          423 a          706 c          731 g          480 t
ORIGIN      
        1 ctccacagtc cagccaaccg gcgttcttca ggattgcgaa acatcaccgg cagccagcga
       61 tcaatcgcag cgcgaatatc cggcagctca gcgtccgcgg ctagcgcatc cagcgtcagc
      121 cgccagacgg ctggattgcc ctggatgagc gccttgtcgg tcgaatcgcg cagacgctgc
      181 atcacgctgc cgcagcgact ggggcaagtc gcgagcaggt attcgatcag gtcgtcgtcg
      241 gtccagggcg cgaggcgcag gacgtcgcag tcccgttcta cgggcttgcg actggcaaga
      301 atcactcggt aaccgctctc cttgaacttc gcctcagttg tgtcatcgag aaagacaagg
      361 cgcgacacgg gaaagagcgt tgccgcaagg tgccgcgatg ccgtcgtttt cccagagccc
      421 ggcccgccca tgatttggat gggccggtcc ccgccacggt agagcaattc ggcgacgact
      481 tcttcgaccg gcagtgcgac cgtgccttcg gggccgaaga ctcgcggccg gacgatcgcg
      541 cgttgcggaa tgaccatgac tgcgaatcgg cgcgtgcgcc gctcctgtca tgtcgttagt
      601 ctgtacgtcg tgcagcacag agtgaattgc gcggacgaac cggaggtcca tcctacgggc
      661 gagcgagcga cgactattcc tggatcgcct ctttcacctt ctgcacgaag tgggggctct
      721 tgccgctggc ggagagttcg gcggctttgg ctttggcggc cttttcctgg gtgaagtcga
      781 acagggcgac gcgcttcagc gactggttga agacgcccca gaagagcttc atgcggatga
      841 cttccggcgt tttgctgcgg ctcttgcgtt tggcgggggg cttcttctcc ttggcggctt
      901 tgaccttttt gccatcgggg ccgacttccg ttccagccgc tttggcctgg gcctctgcgg
      961 cttcagcttc ggctcgtaac tccttgcgat tgacgacttt gcgtgccata gcgcgatggg
     1021 cccagcggga ggggtgattg ccggatcatc cgggcgggcg gtatcttaac cgattctttt
     1081 cgccgcgtgt agggtgtatc agaggagcgc tgtgccgctt atctaaccaa gatcggtcaa
     1141 ctccacatcg tgtagtatcc agggttcgaa ccaacccttc gcgcttcgtg ccgcttcggg
     1201 gaaggccgcg ggcctaatag aattctgcaa ccaacttaac aaaaaagcgc tgctaccgac
     1261 ttcgaagcgc tgatcgaaag tcgggccatc tgaccaacgc tcgacgattg tttgccaact
     1321 tgacggctcc ccgctaacga gccagtagag cgtgactgct tcgtcggtgc cttgtgccca
     1381 aggaaaaaga ccgggtcgcg caggatagaa gtcataggga gaggctgcac gccgcgctcg
     1441 agctgccgat cgaaagagat tccaagcgcg gagaaactcc ggatgtgaac tgggaggatc
     1501 gggctcttca acacacaaca agtggctgtt ttcgatgatt agccgctggt tgttatagcg
     1561 attcctcgaa aatgggttga atatctgcaa gaagccgccc cagcatcccg tgccgtagtg
     1621 ctcgattagc agcttgtagt ccggaggaag ctcgaatccg aagtgagtct cgaccgcgcg
     1681 ccacgacttg tcgttcgggg gatgcacggc tggcggtcga agcggagggg gtgcaaattg
     1741 aagaagcgat tgtaggccgt tgttgtccat cactacactc tgccaatcgg ctgcgctgtg
     1801 gtgctttgca cccgcggcgg gcttgtgcgt cctactcttc cggcagcggc gtggcgccgg
     1861 gcggaggcgt gccgccccct tcgcggacgt acttggcccg catctgggca tattcctcgg
     1921 gcgtcggaaa atcgaccgcg gtctggccgc cttccaccgc ctgctcgaac aaccgttcca
     1981 aaaacggccg gcaccagccg cagcccgttc ccgcaccaaa gcactcgctg agctgcgccg
     2041 gccgcttggg cttctcgatg cggatccaat tgacgacctt tcgcttcgtg acgtgaaagc
     2101 aaaggcagag ttcgtcgtcg gggtgcatgg cttcatcgta caccggacgc catcgctcgt
     2161 ttaacgggta gccgaaggcg ccccgtcgat cggcatcgcc tgcggctcgc cgttaaacga
     2221 ccgcagtcac gctcccgtcg ccagcagcag ggcgattttg cacgcatccc gataggcggc
     2281 caggtcgagc atttcgctgg tcatgtggac gttcatctgg ccgcagccga gcgtgacggt
//
LOCUS       DASUGB010000063         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_702367, whole genome shotgun sequence.
ACCESSION   DASUGB010000063 DASUGB010000000
VERSION     DASUGB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702367"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFV87_01180"
     CDS             complement(<1..342)
                     /locus_tag="VFV87_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102392.1"
                     /translation="MHCNCAIVRLATLLLLATAAAAAAGCSPAARLHGKWAVDTAKLQ
                     AQATGDQKNPLAGLAAGFLSMIQVNLEFKADGTCSASVEALGQTKSTNGAWRFVKADG
                     DSLVISAKMDDA"
     gene            complement(450..959)
                     /locus_tag="VFV87_01185"
     CDS             complement(450..959)
                     /locus_tag="VFV87_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX5102393.1"
                     /translation="MTATHLQHKAQGPQAVRCAVITVSDSRTPETDTGGQAVVVHLTG
                     AGHTVVHREIIPDDPARMRPLLESLRGRDDLDAILLTGGTGVTSRDQTYETVTALLDK
                     PLPGYGEVFRMLSFQEIGPAAILSRATGGLLGRKVLLTMPGSPAAVRLAMEKIIVPEL
                     PHLVREARW"
     gene            1200..2477
                     /locus_tag="VFV87_01190"
     CDS             1200..2477
                     /locus_tag="VFV87_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102394.1"
                     /translation="MAAPERRVGCAHHNAWSLMVGRAHPTRWSLLAAALLAAATTSAR
                     ADLFLLENGGRVEGEWLNREELPRRVYLVKTAAGVTLSLPEADVREAIRQSPAVAEYA
                     KRAPATPDTLDAQWALAEWCRVNRLTHERETHLERIIRLDPNHQDARRALGYQFLQGK
                     WVSRDQHFRELGMEFYRGKWRTPQSIEILESRGRKEVAEKDWAVKLRRWRRDLADPDK
                     ARQAYELIAAIDDPHAVPALASGFRQERDRRVRMLYADVLANIKTSPAVQILVHATLD
                     DGDEEIYYYCLDKLAQLQLPHVGDPYVAALKDASNPRVNRAASALAHLQDRSAISPLI
                     DALITTHQQVQPGTMGPNATSTTFGSGGTVMKQNEGPRILIVQVQNQQVLDALTRLTG
                     VNFSFDQRAWRYWHAQEKIAAEAQQPPLDARRD"
     gene            complement(2481..2987)
                     /locus_tag="VFV87_01195"
     CDS             complement(2481..2987)
                     /locus_tag="VFV87_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5102395.1"
                     /translation="MSGVRNFTDLLIWKRARAWSKEIFWRTQEGAFARDQRLVVQIND
                     SSESVMSNIAEGFGRGTQEEFVVFLGYALGSLMETQSHLCAAYDRKHLAKDEFARLYG
                     GGNELRKMTVGFVRSMIMPRSGVRNVRRVKSWSERTAEIYERLTGKKFVMPRADGTIP
                     TEEEAARS"
BASE COUNT          520 a         1037 c         1028 g          504 t
ORIGIN      
        1 ggcatcgtcc atcttggcgg aaatcaccag cgaatcgccg tcggccttca cgaaccgcca
       61 ggcgccgttg gtcgatttcg tttgccccag cgcctcgacg ctggccgagc aggtcccgtc
      121 ggctttgaat tccaggttca cttggatcat cgacaggaac ccggcggcca gcccggccag
      181 cgggttcttc tgatcgccgg tcgcctgagc ctgcaacttg gccgtgtcga ccgcccattt
      241 gccgtgcagg cgggccgccg ggctgcagcc ggcggcggcg gctgcggcgg ttgccagcag
      301 gaggagcgtg gcgagacgaa cgatggcgca gttgcagtgc atatcgagag ccggagcgag
      361 ttgcggttca gccgcgtttc cgcgcgggaa tctatcgcag gcccctgctg gcggcaattg
      421 caatttgaag tgctagcacg gcgcgcgatt caccaccggg cctcgcgcac caggtgcggc
      481 agctcgggga cgatgatctt ctccatcgcc agccgcaccg ccgccggcga gccgggcatc
      541 gtcagcagca ccttgcggcc gagcagcccg ccggtggccc ggctgaggat cgcggccggg
      601 ccgatctcct gaaagctgag catccggaag acctcgccat accccggcag cggcttgtcg
      661 agcagggccg tcaccgtttc gtaggtctgg tcgcggctgg tgacgcccgt gccgccggtc
      721 agcaggatcg catcgagatc gtcccgcccg cggagcgatt ccagcagcgg ccgcatccgc
      781 gccggatcgt cgggaatgat ctcccgatgc acgaccgtgt gccccgcccc ggtcaaatgc
      841 acgaccaccg cctgcccgcc ggtgtccgtt tccggcgtgc ggctgtcgct gacggtaatc
      901 accgcgcagc ggaccgcctg cggcccctgg gctttatgct ggaggtgtgt ggcggtcatg
      961 gctgcgtata tgccgtaggg tgggacaagc gagcgccgta gggtgggacc agtgagcgcc
     1021 gtagggtggg accagtgagc gaagcgagcg caggcccacc aggcccgccg gtggtgggcc
     1081 cgcggccgcg gcggccttgt cccaccttac acgctgcgtg tgcgtcgcgg tcatggccaa
     1141 acgcttgcgg cagggaaacg gcggccttta tactgcgcgt gtcgttgaag gaccatccga
     1201 tggctgcacc ggaacgtagg gtgggctgtg cccaccacaa cgcatggtcg ctgatggtgg
     1261 gcagagccca ccctacgcgc tggtccctgc tcgcggccgc gctgctcgcg gccgcaacga
     1321 ctagcgcccg ggccgatctg ttcctgctcg aaaacggcgg ccgcgtggaa ggcgagtggc
     1381 tcaaccgtga ggagctgccg cggcgggttt acctggtgaa gaccgcggcc ggcgtgacgc
     1441 tctcgctccc cgaagcggac gtgcgggagg cgattcgcca atcgccggcc gtggcggaat
     1501 atgcaaagcg tgcccccgcc acgcccgaca cgcttgacgc ccagtgggcc ctggccgaat
     1561 ggtgccgcgt caatcgcctg acgcacgagc gcgaaacgca cctggagcgg atcatccggc
     1621 tcgaccccaa ccatcaggac gcccggcggg ccctcggcta tcagttcctg cagggcaagt
     1681 gggtcagccg cgatcagcat ttccgcgagc tgggaatgga gttctatcgc ggcaagtggc
     1741 ggacgccgca gtcgatcgag attctggaat cgcgcgggcg gaaagaagtg gccgagaagg
     1801 actgggcggt gaagctgcgg cgctggcggc gggacctggc cgatcccgac aaggcccggc
     1861 aggcgtatga actgatcgcg gcgatcgacg acccgcacgc cgtcccggcg ctggcgtccg
     1921 gcttccggca ggagcgcgac cgccgcgtga ggatgctgta tgccgacgtg ctggcgaaca
     1981 tcaagacgtc gcccgcggtg cagatcctgg tgcacgccac gctcgacgac ggcgatgagg
     2041 agatctatta ctactgcctg gacaagctcg cccagcttca attgccgcac gtgggcgatc
     2101 cgtacgttgc ggcgctgaag gacgccagca acccccgcgt gaaccgggcc gcttccgccc
     2161 tggcccactt gcaggaccgc tcggccatct cgccgctgat cgacgccctg atcaccaccc
     2221 accagcaggt gcagcccggc acgatggggc ccaatgccac cagcaccacg ttcggcagcg
     2281 gcggcaccgt gatgaagcag aacgagggcc cgcggattct gatcgtccag gtgcagaacc
     2341 agcaggtttt agacgccctc accaggctca ccggcgtcaa cttcagcttc gaccagcggg
     2401 cctggcgcta ttggcacgcc caggaaaaaa tcgccgccga agcccaacag ccgccgctcg
     2461 acgcgcggcg ggattgagcg tcaggagcga gcggcttcct cctcggtcgg aatggttcca
     2521 tcggcacgcg gcatcacgaa cttcttgccc gtgaggcgct cgtagatttc cgccgtcctc
     2581 tccgaccacg acttgacgcg gcggacgttc cgcacgcccg accgcggcat gatcatggag
     2641 cggacgaagc caacggtcat cttccgcagc tcgtttccgc cgccgtatag gcgggcaaac
     2701 tcgtctttgg cgaggtgctt gcggtcgtac gccgcgcaca ggtgcgactg cgtttccatc
     2761 aggctgccca gcgcataacc gaggaagacc acgaattctt cctgcgtgcc gcgtccgaag
     2821 ccttcggcga tgttggacat gacggactcg gacgaatcgt tgatctggac gaccaggcgc
     2881 tggtcgcggg cgaaggctcc ttcctgggtc cgccagaaga tctccttcga ccaggcccgc
     2941 gcccgtttcc agatcagcaa gtccgtgaag ttgcggacgc cggacatggc tcgcctccaa
     3001 gtcgatacag gggctgactc agtactcagt actcagtact cagtactcag tactcagtac
     3061 tcagtactca gtactcagta ctcagtact
//
LOCUS       DASUGB010000064         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_841840, whole genome shotgun sequence.
ACCESSION   DASUGB010000064 DASUGB010000000
VERSION     DASUGB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841840"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFV87_01200"
     CDS             complement(<1..1083)
                     /locus_tag="VFV87_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102396.1"
                     /translation="MLTIFGGRNHRGFCDCVGRRDFLTIGGMALGGLALPQILRAEAT
                     PGAGRSHKAIINVYLPGGPPHIDMWDLKPNAPAEIRGEYSPIKTTVPGMEICELFPKL
                     ASMAQRFAIIRSLCDSDGDHAAFQCMTGRKRSQQPPPGGWPAMGAWASKLKGNVNPSV
                     PAHVSLMYKTGHREWGDPGSGGFLGMGHAPFRLVGGKDNGMKSDNMVLQGVTLDRLHD
                     RDGLRASLDRFRRAADGSGSMGGLDEFADKAMGILTSSELADALDLSQEDPQVLAKYG
                     VDDAEFERDGAPRMVRNFCIARRLVEAGARVVSMNFIRWDWHGGDGMNFVNSKRDFPL
                     LDSGLSSLLTDLHERGMDRDVSVVVWG"
     gene            1442..>2206
                     /locus_tag="VFV87_01205"
     CDS             1442..>2206
                     /locus_tag="VFV87_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102397.1"
                     /translation="MLRRLSFALAVVLICQPIVHAQWQPAKGPLMTRWAKDVSPAKVH
                     PEYPRPQMVRKDWMNLNGLWDYKVAADDGDSLPMKYDGQILVPFPIESALSGVMKRVG
                     PTDVLWYRREFTPPTMPTGGRLLLHFGAVDWRCAVIVNGKEIGEHQGGYDPFTFDITD
                     ALQANKKTQELVVLVGDPTDANWQPRGKQVREPKGIWYTPTTGIWQTVWLEVVPAAYI
                     RGLTITTNLNADPPGAITVRVDADVAGDDAGKYRISL"
BASE COUNT          390 a          716 c          716 g          384 t
ORIGIN      
        1 gccccagacg acgaccgaga cgtcgcggtc cattccgcgc tcgtgcaagt cggtcagcag
       61 cgaggagagc ccgctgtcca gcagcgggaa atctcgcttc gagttgacga aattcatgcc
      121 gtcgccgccg tgccagtccc agcggatgaa gttcatcgag acgacgcggg cccccgcctc
      181 gaccaggcgg cgggcgatgc agaaattgcg gaccatccgc ggcgcgccgt cccgttcgaa
      241 ctccgcgtca tcgacgccgt atttcgcgag gacttgcggg tcttcctgcg agaggtccag
      301 ggcgtcggcc agctcggacg acgtgaggat gcccatcgcc ttgtcggcga attcgtcgag
      361 cccgcccatc gagccgctgc cgtcggctgc ccggcgaaag cggtcgagcg aggcccgcag
      421 gccgtcccgg tcgtgcaggc ggtcgagcgt caccccttgc agcaccatgt tgtccgactt
      481 catgccgttg tctttgccgc cgacgaggcg gaacggagcg tgccccatgc ccagaaaacc
      541 gccgctgccg ggatcgcccc attcgcggtg gccggtcttg tacatcaggc tgacgtgggc
      601 cggcaccgac ggattgacgt tccccttcag cttgctggcc cacgccccca tggccggcca
      661 gccgccgggg ggcggctgct ggctgcgctt gcggccggtc atgcactgga aggcggcgtg
      721 atcgccgtcg ctgtcgcaaa gcgagcggat gatcgcaaac cgctgggcca tcgacgccag
      781 cttgggaaac agctcgcaga tttccatgcc cggcacggtc gtcttgatcg ggctgtactc
      841 gccgcgaatt tccgccgggg cgttgggctt gaggtcccac atgtcgatgt ggggcgggcc
      901 gccgggcaga tagacgttga tgattgcctt gtgcgagcgg ccggcgcctg gggtcgcttc
      961 ggcccgcagg atttgcggca gcgccagtcc gccgagggcc ataccgccga tcgtcaggaa
     1021 atcgcgccgg ccgacacagt cgcaaaagcc gcggtgattt cggccgccaa aaatggtgag
     1081 catggcgtgg tgttccaagc gaggcgggat gggtaggtag gactgcacag ccgcgaacga
     1141 tgcatagccg cgaccagcgg tcgcgccacc catgcaaagc cgcgaccagt ggtcgcgccg
     1201 gcaccctgat aatcgcaaac cgggaggcaa agtcaacacc gatccatcct tgtttatcgg
     1261 attctttctg cccttggcca tacaaagccc ggaccattgg tcagggtgtt tcgcaaacag
     1321 atttttcact ccatcgaccg accgccagaa ttcgccctgc cagcgaaaca cccgaccact
     1381 ggtcggggct ttgggcgcgg actacggtaa tgtggttcaa tcaccgctgg gagcctctcg
     1441 catgctgcgc cgcctttcct tcgcgctcgc tgtcgtattg atctgccaac cgatcgtgca
     1501 tgcccagtgg cagccggcca aggggccgct gatgacccgc tgggcgaagg acgtttcgcc
     1561 cgcgaaggtg catcccgaat atccgcggcc gcagatggtc cgcaaggact ggatgaatct
     1621 caatggcttg tgggattaca aggtcgcggc tgacgacggc gattcgctgc cgatgaagta
     1681 cgacggccag atcctcgttc ccttcccgat cgagtcggcc ctatccggcg taatgaagcg
     1741 ggtcggaccg acggacgtgc tttggtatcg ccgcgagttt actcccccga cgatgcccac
     1801 cggcggtcgc ttactgctgc atttcggcgc cgtcgattgg cgctgcgcgg tcatcgtgaa
     1861 tggcaaagag atcggtgagc accaaggggg ctacgacccc tttacgtttg acatcaccga
     1921 cgccctccag gcgaacaaga aaacgcagga gctggtggtg ctggttggcg atcccaccga
     1981 cgccaactgg cagccccgag gaaaacaggt ccgcgagccc aagggcattt ggtacacgcc
     2041 gaccaccggc atctggcaga cggtgtggct ggaggtggtg ccggccgcgt acattcgcgg
     2101 tctcaccatc acaacgaacc tgaacgccga cccgccgggg gcaatcacgg tacgcgtgga
     2161 tgccgatgtc gcgggcgacg acgcaggcaa gtaccgcatc tcgctg
//
LOCUS       DASUGB010000065         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_563508, whole genome shotgun sequence.
ACCESSION   DASUGB010000065 DASUGB010000000
VERSION     DASUGB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563508"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFV87_01210"
     CDS             <1..437
                     /locus_tag="VFV87_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102398.1"
                     /translation="APGSAPPERMMVLVLLLLSILPRPGASRETCDVIELNHFYNELG
                     QPVFDQILFWEWSGSRYDVRAWRLVQQPEQLPRRDWRGGGFVALWSDGPALQPTATPP
                     LREVFREVHAPSFRETWTQHDPELEERQFLPVQLRRGLKGER"
     gene            complement(448..990)
                     /locus_tag="VFV87_01215"
     CDS             complement(448..990)
                     /locus_tag="VFV87_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5102399.1"
                     /translation="MKKPPDIRTFLTRLARRMAFVGQCHSASGHAFRLQIAASYEPTI
                     IEHSRELQRLFDELQAARAAWETLTGTGAPRMPVTIQQWGSTCAWPPAPPPRGLLEDV
                     RKSVPGSAGVYFLWEAERVAYVGKSENLRGRLTMGHEHLLATDLLSWLDFDPGDLFFA
                     EAFYIGAYRPHRNGGTSRLA"
     gene            complement(990..1220)
                     /locus_tag="VFV87_01220"
     CDS             complement(990..1220)
                     /locus_tag="VFV87_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102400.1"
                     /translation="MPRPTLDVDQTTFAGQVAAEIRRRRLKKFPRAEEAAEAAGVPVK
                     TWYGWETGKVHLDALPLAAAALGCSPRSLLPS"
     gene            complement(1220..1390)
                     /locus_tag="VFV87_01225"
     CDS             complement(1220..1390)
                     /locus_tag="VFV87_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102401.1"
                     /translation="MSWRDEEQHLIDAAIAAAEDCAECGVPIGFGSCLGCQAVAASCP
                     ELFEDEEYEDDR"
     gene            complement(1393..1680)
                     /locus_tag="VFV87_01230"
     CDS             complement(1393..1680)
                     /locus_tag="VFV87_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102402.1"
                     /translation="MPGDGSCCDHCGWTLKGGGDDPRLPSNRSLEQIAQETNRRTAQR
                     ARMALDRASSYLREAIAAISILPDTPGNQSLHENAGKIEERIRHLQSCVRA"
     gene            complement(1722..1985)
                     /locus_tag="VFV87_01235"
     CDS             complement(1722..1985)
                     /locus_tag="VFV87_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102403.1"
                     /translation="MPHETPAHAEDGQDLALALEAAGGIDRITIDVVEGGFIWRLAKG
                     GREIFASGPDEPDQVASMLQSCEVLLNTELGIAGERLCQPAFN"
     gene            complement(2115..2630)
                     /locus_tag="VFV87_01240"
     CDS             complement(2115..2630)
                     /locus_tag="VFV87_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5102404.1"
                     /translation="MGSKRDYQKATLPVVRARARVVFQGHADVRDKVNDAISVGWELS
                     LTAPPGTPPKSIAYYACLQVKIGRQFRQSERSIDGPNPRRRKKPQREVADVGKVLARP
                     GNNPAAIATVLMDYTAWLPSLTDREQELLEAFLRGDSTKELARRYRISPARVSQIRRE
                     LVEYWQAFTSE"
     gene            2907..>3116
                     /locus_tag="VFV87_01245"
     CDS             2907..>3116
                     /locus_tag="VFV87_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102405.1"
                     /translation="MAQLEALYLAAVAALDAGDYDTAIVKAQACKVRLATTPNVDRSL
                     GGGGRQQMQWTNAAALDSFIRECKLL"
BASE COUNT          522 a         1006 c         1030 g          558 t
ORIGIN      
        1 atgcacctgg ctctgctccg ccggagcgca tgatggtttt ggtgctgttg ctgctgtcga
       61 tcctgccgcg cccaggcgcg tcgcgcgaga cgtgcgacgt gatcgagctg aaccactttt
      121 acaacgagct ggggcagccc gtcttcgacc agatcctctt ctgggagtgg agcggcagcc
      181 ggtacgacgt gcgggcgtgg aggctcgtgc aacagcccga acagctcccg cggcgcgact
      241 ggcgcggcgg cggctttgtc gcgctctggt ccgacgggcc ggcgctgcag ccgacggcga
      301 cgccgccgct gcgcgaagtc ttccgcgaag tccacgcgcc cagcttccgc gaaacgtgga
      361 cgcagcacga tccggagctg gaggagcggc agtttcttcc ggtgcagctc cgccgcggcc
      421 tgaaaggcga gcgatgagca ccaatagtca cgcgagtcgg ctggtgccgc cgttgcggtg
      481 cggacggtac gcgccgatgt agaacgcttc ggcgaaaaag aggtcgccag gatcaaagtc
      541 gagccacgag agaaggtcgg tcgcaaggag gtgctcgtgg cccatagtaa gccgccctcg
      601 caagttctcg ctcttaccga cgtacgcgac gcgctccgct tcccatagaa aatagacgcc
      661 ggccgaaccg ggaacgctct tgcgcacgtc ttcgaggagt ccgcgcggcg gcggcgctgg
      721 cggccatgcg cacgtgctac cccattgttg gattgtcacc ggcatccgcg gcgcgccggt
      781 gccggtcaga gtctcccacg cagcacgcgc ggcctgcaac tcatcgaaga ggcgctgcag
      841 ctctcgcgag tgttcgatga tggtcggctc gtacgatgcc gcgatttgca accggaacgc
      901 atgcccgctc gcgctgtggc actgcccgac gaaggccatg cggcgcgcga gacgcgtgag
      961 aaacgtgcgg atgtctgggg gtttcttcat cacgaaggaa gcaacgaacg cggtgagcag
     1021 ccgagggcgg cggcggcgag tgggagggca tcgaggtgga ccttgccggt ttcccagccg
     1081 taccaggttt tcacggggac gccggcggct tcggccgctt cctcggcgcg ggggaacttc
     1141 ttgagccggc ggcggcgaat ctcggccgcc acctggccgg cgaacgtggt ctgatcgaca
     1201 tcgagcgtgg ggcgtggcat cagcggtcgt cctcgtattc ctcgtcttcg aacagctcgg
     1261 ggcacgacgc ggcaacggcc tgacagccga ggcacgagcc aaagccgatc ggcacaccgc
     1321 actctgcgca atcttcggcc gccgcgatcg cggcgtcgat gagatgttgc tcttcgtctc
     1381 gccagctcat agtcaggccc tcacgcacga ttgcagatgc cggatgcgct cttcgatctt
     1441 gccggcattt tcatgcagcg actgattgcc cggcgtgtcg ggcagaatgc tgatcgccgc
     1501 gatcgcctca cgcagatagg agctcgcgcg atccagcgcc atgcgagcgc gctgagcggt
     1561 tcgccggttc gtctcctgcg cgatctgttc caggcttcga ttgctgggaa ggcgcggatc
     1621 gtcgccgcca cccttcagcg tccaaccgca atggtcgcaa cacgacccat cgccgggcaa
     1681 cgggtgctcg caatctgggc atctcataaa acctccgatg gttagttaaa cgccggctgg
     1741 cacaggcgct cgccggcaat tcccagttcc gtgttgagca gcacctcgca ggactgcagc
     1801 atgctggcga cctggtctgg ctcgtcgggc cccgaggcaa aaatctcgcg gccgcctttg
     1861 gccagccgcc agatgaaacc gccttcaacg acgtcgatcg tgatccggtc gatgccgccg
     1921 gccgcttcca gggcgagcgc caggtcctga ccgtcttccg catgcgcggg cgtttcgtgt
     1981 ggcatgatac atattctcct aacgggcgac aactgggcgc ggccggagtg gccgccctgg
     2041 ggaatctggc gcatgccgcg gcggaattta gcgcggggag gaaaaaacgg cccgcttccc
     2101 tgcgggccgc tgggtcattc gctggtgaaa gcctgccagt actccaccag ctcgcgtcga
     2161 atctgcgata cgcgggcggg tgagattctg tagcggcggg cgagttcctt cgtggaatcg
     2221 ccccgcaaga aggcttccag aagctcttgt tcgcggtctg tcaggctggg gagccaggcg
     2281 gtgtaatcca tgaggaccgt ggcgatggcc gccggattgt tgcccggccg ggccagcacc
     2341 ttccccacgt cggccacttc ccgctgcggc tttttacgcc gccgggggtt gggaccgtcg
     2401 atcgaccgct ccgactggcg aaactgccgc ccgattttca cctgcaagca cgcatagtag
     2461 gcgatgctct tgggtggagt tccgggcggg gcggtgagcg atagctccca gcccacgctg
     2521 atcgcatcgt tcactttgtc ccgcacgtcc gcgtggcctt gaaacaccac gcgggcccgc
     2581 gctctgacca ccggcaaggt ggctttctgg tagtcgcgtt tggagcccat aacgattctc
     2641 cctcagaagg tctgaatcgc cggccggacc ttccggccgg cctctcccac tcaccgcaac
     2701 tgagggaacc cgtcagggcc cgctgccatt tccagtcgcg accaaattgt caaagagcgt
     2761 tgcttcctac aagagagtat tcgccgggtg ggagaatata ttgatgcggc aaagcggaaa
     2821 aatccgggaa tttcgacccc tggttacaga tctgtaacca acttccttga ttggccgcgg
     2881 gtcggggcaa cgctggaagt atgagcgtgg cgcagcttga ggccctctac ttggcggcgg
     2941 tcgccgcctt ggacgccggc gactacgaca cggcgatcgt caaggcgcag gcctgcaagg
     3001 tccgcctggc gacgacgccc aacgtcgatc ggtcgctcgg cggcggcggc cggcagcaga
     3061 tgcagtggac caacgccgcg gccctcgact cgttcatccg cgagtgcaag ctcctc
//
LOCUS       DASUGB010000066         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_285844, whole genome shotgun sequence.
ACCESSION   DASUGB010000066 DASUGB010000000
VERSION     DASUGB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285844"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFV87_01250"
     CDS             <1..449
                     /locus_tag="VFV87_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102406.1"
                     /translation="FVPFEGTLGARRLPSIKAFAAEPIAEWMETHARRDLPGRLLTTF
                     EYGSYLKWRLPSLSESIDSRNIFPDSVVLSVDDSIDPPRLGPWRSADVAVIPVDYPLA
                     TTLDRDSSWMRIMVAPPPPWAPDAKRAGLWVRRSWWMVASQTAATS"
     gene            683..756
                     /locus_tag="VFV87_01255"
     tRNA            683..756
                     /locus_tag="VFV87_01255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:717..719,aa:Arg,seq:cct)
     gene            797..1462
                     /locus_tag="VFV87_01260"
     CDS             797..1462
                     /locus_tag="VFV87_01260"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5102407.1"
                     /translation="MTTPTLEAPVREPKPESFLDWFRINSRIVGVAVAVVVAVAFGFW
                     FYNRSAVLKAENADKKLLAAKQSIGSGNMPLAQSDLKKVADQYAGTGAGAEAGMLLAQ
                     LKLDAGDNQGAVTVLTDLSHKVPTGPYAASIKGMLGDAYVQLGKPAEAAAAYEQAALA
                     TALPNERAMLMSKLGRAWLAAGKPDKARETWAALAAQQANPALAAEARLRLGELLATA
                     QKK"
     gene            complement(2633..>2864)
                     /locus_tag="VFV87_01265"
     CDS             complement(2633..>2864)
                     /locus_tag="VFV87_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102408.1"
                     /translation="RTYERGVEGETLACGTGAVASAILLREHEETSDGAALTTRSGRV
                     LRVRLSERTLSGWHPSLSGEGRIVFQGVLGEL"
BASE COUNT          532 a          891 c          980 g          461 t
ORIGIN      
        1 gctttgtgcc gttcgagggt acgctgggcg ctcgacgact tccgtcgatc aaggcgttcg
       61 cggccgagcc catcgcggag tggatggaga ctcatgcccg acgcgatctg ccgggcaggc
      121 tgctcacgac cttcgaatac ggcagctatc tgaaatggcg gcttccctcg ttgagcgagt
      181 cgatcgacag caggaacatc tttcccgact ccgtcgtgct gtctgtcgac gacagcatcg
      241 accctccgag attgggcccc tggcgctcgg ctgacgtcgc cgtcattcct gttgattacc
      301 cgttggcgac gaccctcgac agggattcgt cgtggatgcg gatcatggtg gcgccgccgc
      361 caccctgggc ccctgacgcc aaacgcgccg gcctatgggt acgtagaagc tggtggatgg
      421 tggcctcgca aacggcagcc acgtcatgac ttcgatcggt cgcggagcgc gttcggcctc
      481 gtggctgccc attcgcgatc tgcggggtag caccgttcgt gagatatggg ggccgctggc
      541 gagcggtgca tgggttgcct agttttgaag gctgcacccg cgccgccgga tactgaatct
      601 cgacgatgca ccgcagaacg attcgcggac gctgagagat ccggttaagc gaccagcgta
      661 agcctaagca acagttaacg ttgcccctgt agctcaggtg gatagagcag cggtttccta
      721 aaccgttggc cgggtgttcg agtcacccca ggggcatgac ggcctgatct cagcgaaccc
      781 agaacagcag cgagcaatga cgaccccgac gttggaagca cctgtccgtg agccgaaacc
      841 cgagagcttt ctcgattggt tccgcatcaa ctctcgcatc gtcggtgtcg ccgtagccgt
      901 ggtggtggcg gtggcgttcg gcttctggtt ctacaaccgg tcggcggtcc tgaaggcgga
      961 gaacgcggac aagaagctgc tcgccgccaa gcagtcgatc ggcagcggca acatgccgct
     1021 ggcgcagagc gacctgaaga aggtcgccga ccagtatgcc gggacgggcg ccggcgccga
     1081 ggcagggatg ctgctcgcgc agctgaagct cgatgccggg gacaatcagg gcgcggtgac
     1141 ggtcctgacg gacctgtcgc acaaggtgcc cacggggccg tacgccgcct cgatcaaggg
     1201 catgctgggc gacgcctacg tgcagctcgg aaagccggcc gaggcggccg cggcgtacga
     1261 gcaggccgct ctggcgaccg cgctgccgaa cgagcgggcg atgctgatgt ccaagctcgg
     1321 ccgggcctgg ctggcggccg gcaagccgga caaggctcgc gagacgtggg ccgcgctggc
     1381 ggcgcagcag gcgaacccgg cgctcgccgc cgaggcgcgg ctccggctgg gcgagctgct
     1441 ggcgacggcc cagaagaaat agctgaaggc tgggcgcctc cggggcgccg cattccaaga
     1501 tcagaggagc cagcgcacag gcgctggctc ttcgttttct ttgatctcga gcaaccgtgg
     1561 ggcgatcggt gccgagcttg gggcggccga attggcagcc ggccaaccca ggagtccagg
     1621 ccagacttct ccccggaagt tgacacgacc tggctagggg ggcggcggcc ggaagcgaag
     1681 gcgggagctc ccactgccgg tcgagccgga cgcggacgag tccaggacga ggcggctcgc
     1741 cgggggcctg cgatcccttc aggcaaacgc tttcgtgcgc gcttgcgacc ccaaggcgtg
     1801 tcatgccgga cgcgtcgtgc ttgaggtatt cggcgctacg gccaaggccg tcggagtggg
     1861 ctgcaaagtt gtgtgtaacg gtgtgggcga aatccagcag gatgtccgca acgcgtgatc
     1921 cgagtccttg ccacgccagt ccgaaaacca gttcgcgatg aaatgtcgac gccaagaaag
     1981 cgctgaagct tttctggctc ggcgacaaga aagccgaacc agcagaacag ccacgtccgc
     2041 ctggtcctta accgaactcg gcttgggttc aacggcgaag ttcgaacgcc agatggaagc
     2101 gttggcgagt cgattctcgc cggtagaggg aacgaggtac agcgcagtcc gaaaccagcg
     2161 tcagactagg ggccgataag gcgacacaaa ggcgacagcg gactcaacag tgcgcccacg
     2221 catgttcgta taagatgtat tatgtaaacc agtgccggtg gaaaaatgtg gagaactccc
     2281 gggcatactg cggtcatgtc gaggtaatcc acgtccccac ctccgcttag gcatgtgatc
     2341 tcatccagtt tctgatcaag cgagatcaag cgagcaatcc agcggactct ggctcgaggc
     2401 gatcgagcgc ccggaagtcg ctgtcgcggt cgctgtcgca ttcacaggcg aaatccagcg
     2461 gctggggagg cgaatccagc agctccgagg gccaagaaaa gggccaaaat cccgtgtcac
     2521 tcagtgccct ggcccgcagc gcggcggtcc gaacaggacg ggacttggag ggtcaccagg
     2581 gggccggatc ccggcccgac atcggcctgg ccggcgatgc ccgccggcgt gctcagagct
     2641 cgcccaggac gccctggaag acgattcggc cctcgcccga tagcgacggg tgccagccgc
     2701 tgagggtgcg ctcggagagg cgcacgcgga ggaccctccc cgaccgggtc gtcagcgccg
     2761 cgccgtccga ggtctcttca tgctcccgca ggaggatcgc cgaggcgacg gcaccggtgc
     2821 cgcatgccag cgtctccccc tcgacgccgc gctcgtaggt ccga
//
LOCUS       DASUGB010000067         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_980883, whole genome shotgun sequence.
ACCESSION   DASUGB010000067 DASUGB010000000
VERSION     DASUGB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980883"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..960)
                     /locus_tag="VFV87_01270"
     CDS             complement(217..960)
                     /locus_tag="VFV87_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX5102409.1"
                     /translation="MTPPQQIDVFLLPSLAEAENLTGRVCVVIDVLRATTTIVHALAA
                     GAKEVVPCLEVDEARRIAAEIGPAAVLGGERAGGKISGFDLGNSPAEYTPGVLAAKTL
                     VFTTTNGTRALARCQAAERVLIGAFVNFSAICGELAASERVALICGGTDGEVTREDAL
                     LAGAIVVELTQSRRAALNDQAEIAADAWRTAVRLLTDRPLGMTLRDSRGGRNLIGIGH
                     ESDIDLAAQIDRFHIVPELDLATWRIRLP"
     gene            1069..1893
                     /locus_tag="VFV87_01275"
     CDS             1069..1893
                     /locus_tag="VFV87_01275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5102410.1"
                     /translation="MSSSAGSGPVPSPSAEPARFTGKVALVTGARDRGIGGEIALRLA
                     REGAALALISRHESDKLLKKLGKLKARVVHIIGDITKLDDIDRLIGECLAQFGRLDVV
                     VNNAGVELAKSLADFDDSQWRELLNINLSGSIAVTKAALPHLQKPGGAIVNIASALGL
                     AGCRGFSIYSASKAGLIGFTQSLAWELAPQGVRVVALAPGLVYTPMIHKHISQLTPEV
                     MAEIEACHPLGVGRPDDVAAAVAFLASEDARWITGITLPLGWAPHYPLPTWRVMDG"
     gene            1903..>2243
                     /locus_tag="VFV87_01280"
     CDS             1903..>2243
                     /locus_tag="VFV87_01280"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5102411.1"
                     /translation="MALLIDGYNLLHAAGIFSASGPPTLEAARVALLDFLVESLPAKE
                     LARTGVVFDAAHAPPGLPREIEHRGLAVRFARRGGSADELLEELIAAEPDPRNLLVVS
                     SDHRVQRAARQ"
BASE COUNT          374 a          760 c          719 g          390 t
ORIGIN      
        1 cgacaacaat cgcacccaaa tcctggcctc gatcttcgcc taccaactcc tggtgcgttt
       61 caaccatcgc tgcggccgcc aaaacggccg cgtacgatgg atcatggacg cgttgtaaat
      121 ttcaggacac cctcaggctc ctgcctggga acgcacgtcc gcgaggctcc tgcctcgcta
      181 ctaagacgca gtttaccggc caaggttcag atcggtctat ggcaggcgga tgcgccaggt
      241 ggccaggtcc agttcgggca cgatgtgaaa ccggtcgatt tgggccgcga ggtcgatgtc
      301 gctctcgtgc ccgatgccaa tcaggtttcg tccgccgcgg ctgtcgcgaa gggtcatgcc
      361 gagcgggcga tcggtgagca ggcggacggc ggtgcgccag gcatcggcgg cgatttcggc
      421 ctggtcgttc agggcggctc ggcgggactg tgtcagctcg acgacgattg ccccggccag
      481 cagggcgtcc tcgcgcgtca cctcgccatc cgtgccgccg cagatcaggg cgactcgctc
      541 gctagcggcc agttcgccgc agatggccga gaaattgacg aacgccccaa tcagcacgcg
      601 ctcagccgcc tggcagcggg ccagcgcccg cgtcccattg gtcgtggtga agaccagcgt
      661 cttggcggcc agcactccgg gcgtgtactc tgccggcgag ttgcccaggt caaatccaga
      721 aatcttgcca ccggcccgct cgccgcccag cactgccgcc ggtccgattt ctgcggcaat
      781 tcgccgggcc tcatcgactt ccaggcaagg cacgacttct ttcgccccgg ctgccagcgc
      841 gtggacgatc gtcgtcgtcg cccgcagcac gtcaatcacg acgcaaacgc ggccagtcag
      901 gttctccgcc tccgcgagcg acggcagcag aaagacgtcg atctgctgcg ggggtgtcat
      961 gcaggggtaa gtataatggc gttgacgcta ggtccttcgg ctggagaatc agtctatcgg
     1021 ccgatcctcc acggcaacag gcagcttgct cagcccccga tttgcatcat gagttcctcc
     1081 gccggctccg ggccagtgcc atcgccctcc gcagaacccg cccgcttcac cggcaaggtg
     1141 gcgctcgtca cgggggcccg cgaccggggc atcggcggcg aaatcgccct gcgattggcc
     1201 cgcgaaggag cggcgctggc actcatttcc aggcatgaat cggacaagct cctgaaaaag
     1261 ctcggtaagc tcaaagcgcg ggtcgtccac atcatcggcg acatcaccaa actggatgac
     1321 atcgaccgat tgatcggcga gtgcctggcg caattcggcc gcctcgatgt cgttgtgaac
     1381 aacgccggcg tcgagttggc caaatcgctc gccgattttg atgactccca gtggcgcgag
     1441 ctgctcaata tcaacttgag cggctcgatt gcggtgacca aagccgcact gccgcatctg
     1501 caaaagccgg gcggcgcgat cgtgaacatc gcctcggcac tcggcctggc cggctgccgc
     1561 gggttctcga tctatagcgc cagcaaggcg gggctgatcg gctttacgca gtcgctggcg
     1621 tgggagctgg cgccgcaagg ggttcgggtc gtcgccctgg caccgggcct ggtctatacg
     1681 ccgatgatcc acaagcacat ttcgcagctc acgccggagg tgatggccga gatcgaagcc
     1741 tgccacccgc tgggcgtcgg ccggcccgac gacgtcgccg ccgcggtcgc ctttctcgct
     1801 tccgaagacg cccgctggat caccggcatc acgctccccc tgggctgggc gccgcactac
     1861 ccgctgccga cgtggcgggt gatggatggc taaaataggt ccatggccct gctcatcgac
     1921 ggctacaacc tgctccacgc ggcgggaatt ttttccgcga gcggacctcc gacgctggaa
     1981 gcggcgcggg tcgctctgct cgacttcctc gtagaatcgc tcccagccaa ggaactggcg
     2041 cggacgggcg tcgtgtttga cgcggcgcat gcgccgccgg gcctgccgcg cgagatcgag
     2101 caccgcgggt tggcggtccg cttcgcccgc cgcggcggct cggccgatga gttgctggaa
     2161 gagctaatcg cggctgagcc cgaccccaga aatctgctgg tcgtttcgag cgatcaccgc
     2221 gtgcagcgcg ccgcccgcca gtg
//
LOCUS       DASUGB010000068        11074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1675173, whole genome shotgun sequence.
ACCESSION   DASUGB010000068 DASUGB010000000
VERSION     DASUGB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675173"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VFV87_01285"
     CDS             complement(<1..1345)
                     /locus_tag="VFV87_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102412.1"
                     /translation="MRAAVPWAGAGQASPGRIDDERFLRRATLDIIGRLPTPEETTAF
                     SLDPSSDKRAKIALKLLAQAEFGENWGRYWRDVIMYRKTEDRSGIINGPLTTYLTESF
                     NTDKPWSQIATEFITAEGDATENGACGLIVAQQGNPEEVTSEVSRIFLGVQISCAQCH
                     NHPTDRWKREQFHELAAFFPRVSSRIILNNQETGPQVIVSANDMAFGGRFRAPMNMRR
                     FGTPEHYMSDLKNPQSRGTLMTPVLFATGQKLPLGTKDADRRGTLAQWITAKDNPYFA
                     QAFVNRMWSELTGEGFYEPVDDMGPDRTAVAPQTLEYLAKEFAESDYNVKWLFQTIMA
                     TDLYQTPSAPRRGPEDPPMQHNVAQRLRADVLFDNLLVALEASEPRGFGGGRGGLYGF
                     GGGPRGQFAAAFGYDPSQRRDEIQSSIPQALAMMNSPTIAGSLRGTGNTLLARLLR"
     gene            complement(1775..3766)
                     /locus_tag="VFV87_01290"
     CDS             complement(1775..3766)
                     /locus_tag="VFV87_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5102413.1"
                     /translation="MNARTIPLVCLLACVPIAPAAAEDLPPIPPIQRVLPPEGLEIPA
                     EIRQRLEARLAATKKRLEGAEDSVYRADVEVFTKAVELALLHREFYAEKDFAKADWAL
                     DEANKRLDQLADKKAPWERTPGLVVQGYRSEIDGSAQPYGLVIPADHDFGKPVPLYVW
                     LHGRGDKNTDLHFLQERATKPGQIAPPGAIVLHPFGRHCVGWKHAGEIDVFEAIASVK
                     KRYRIDNDRIVLIGFSMGGAGAWHIGAHYADQFVAVSPGAGFAETARYQRLTPDKYPP
                     WYEQKLWGLYDVPGYVRNLFNTQVIAYSGENDKQIQAARVMEEAYLAEGRTLTHLIGP
                     GVEHKYEPQTLAELLKRLEAAATKGRELKPTEVHLQTQTLKYGQYRWVKALRLEEHWN
                     DSRVDAEIVGPDLVRVTTKNVSLLELSPPMDLYGKRVLVDGTEIRVNRRIGDSDAATL
                     VKVLDTWKLAEEADLYSSPLAKRPSLQGPIDDAFMEAFMVVAPTGKSRNPAFQAWCDV
                     ELRHFQNRWRALMRGELPVVHDRDVDPELLPLKNIILWGDADSNSVIRHLEEKLPIKF
                     AEGKWSLGNSTFDGNRFAPVCIYPCLRPGQAPHYVVLNSGLTFREGHDRTNSLQNPKL
                     PDWAIIDITQPPDAFSPGRIHDAGFFDEKWQLKNQPKAP"
     gene            complement(4252..4491)
                     /locus_tag="VFV87_01295"
     CDS             complement(4252..4491)
                     /locus_tag="VFV87_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102414.1"
                     /translation="MRLRGTLRWLSNPRGMLNYLSCVSVLLKDFKEFAVDCTTRIRRG
                     SQCLALASGRPFHYLNSSTVREEELVEQIAITDQV"
     gene            4744..6198
                     /locus_tag="VFV87_01300"
     CDS             4744..6198
                     /locus_tag="VFV87_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5102415.1"
                     /translation="MSSVVLSLCLAANAAGEQKPNIVLIFADDLGINDLSCYGRQDQR
                     TPRLDRMAAEGVRFTCAYCSQPICSPSRAGILTGKNPARLHITTFLPGRPDAPSQRLL
                     HPQIAMQLPLEEMTLAEYLKEAGYATACIGKWHLGGAGFGPKEQGFDYVHAGQANTKP
                     SATEGGKGEYDLTRHAVEFIEQNKNRPFFLYLPHNTPHIPQAAKPELVEKYKDSFNPV
                     NAALMETLDDCVGIVLDKLKELKLDENTIVIFTSDNGGLHVLEFPGGPSTHNTPYRGG
                     KGFLYEGGLREPAIVRWPGKIKPAVIDTPTVNLDWAPTLLTLAGLKIPVGIDGQDISP
                     LLLGGKIDMPPRVFYWHFPHYNNQGGRPAGAVREGDWKYILSYDTGRVELYNLATDVA
                     EEHDVAVDNGAVIARLEALRSDWLKSIDGQNNASNPNFDRTLFHKLYEEFDPSRPILR
                     TTAAGMEEDMKEWRALMNEVVRGNRPAKAKAKQQ"
     gene            6290..7495
                     /locus_tag="VFV87_01305"
     CDS             6290..7495
                     /locus_tag="VFV87_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5102416.1"
                     /translation="MNPARTSFMQVGVLKETFPGERRVALVPADLPKLAKAGMGVIVE
                     PGAGAAAGFADKAYEEKGAKFAFNRAGVFEAEIVVQVRSLGANLSAGRADLPLLRSGQ
                     ILLGTADPLGEPQAVDEIAKTGATFFALELIPRTTRAQAMDVLSSQATIAGYRAVLLA
                     AIELPKIFPLLMTAAGTLTAAKAFVIGVGVAGLQAIATAKRLGAVVRAYDVRPACREQ
                     VESLGAKFVELPLESAAAEVQGGYAAVQDESFYRRQRELLSQVVAESDVVITTAAIPG
                     KMSPLLVPADAVAKMQPGSVIVDLAAERGGNCELTKANERIEINGVTILGPTNLPSEV
                     PTHASQMFSANVTNFLLNLVKKGQIVLNLDDEIIRETLAAEGGQPVHARVRESLGLPK
                     LPEPAPLET"
     gene            7683..8045
                     /locus_tag="VFV87_01310"
     CDS             7683..8045
                     /locus_tag="VFV87_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5102417.1"
                     /translation="MAHAEPPPASVPSLASADSSLAQSTAEKERPPLVLSLTIFVLAI
                     FVGFELISKVPATLHTPLMSGSNAISGITVVGAIIVAGAIGVATPSLGFVATVLAMIN
                     VVGGFLVTHRMLRMFKRR"
     gene            8083..9501
                     /locus_tag="VFV87_01315"
     CDS             8083..9501
                     /locus_tag="VFV87_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5102418.1"
                     /translation="MFDLAYLAAALLFIFGLKNMAHPRTAVRGNLLGALGMAVAIAAA
                     VVFAVLDPQITGFSYGVIGAALALGTAIGAVLALRVQMTAMPQMVALLNGFGGLASVL
                     VAGADLIHDPQKLAADKAGLEVLIAVAASAIVGAVTFFGSLLAYGKLEEFKILRQPIR
                     YPLQQPLNAVLGLILLGLSAAMVYYGLQGDAALTVTMFVLVTLVASVLGLTLVNPIGG
                     ADMPVVISLLNAYSGIAAGMTGFVIANNVLIISGALVGASGIILTQIMCKAMNRSLLN
                     VLFGTLTDSGTSAKAGDIYARVRSTTPEDVALLLDGAQRVVVVPGYGMAVAQAQHAVR
                     ELGQTLEKRGVTVEYAIHPVAGRMPGHMNVLLAEADVPYDKLLEMDQINPTLDTVDVA
                     IVIGANDVVNPVAKTDPKSPIAGMPIIEVDRCRTVVVIKRSLSPGFAGIPNPLFAADN
                     TLMLFADGKDAVLEIVKAVKEG"
     gene            complement(9533..11056)
                     /locus_tag="VFV87_01320"
     CDS             complement(9533..11056)
                     /locus_tag="VFV87_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5102419.1"
                     /translation="MTITQWLICVIAAIGFAFDIYELLMLPLIARPALLELGGIRPGT
                     TEFTTWISLLFYIPAVAGGIFGLMGGYLTDRLGRRRVLTWSILLYAGSAFAAGFSTNL
                     YMLLLFRCLVFVGVCVEFVAAVAWLAELFDNPKRREAVLGFTQAFSSIGGLSVAIAGT
                     IVAGWAVNKSPTILFGLITLPALQLPEVQLPNFLSFLGEIKNGHADWRYLLMSGLIPA
                     IPLIVIRPFLPESPAWQKKRESGTLRRPNIAELFAPKLRRTTLITTLMFTCSFAAAFG
                     AIQQMPLIVPGLPDVRAASDAALEKGFPKAAQEGMRKKLIADGKTEEAADKAVLGKMR
                     SITGPVEQPKITKATEGQEIGGLVGRFAIAILLLFIVSRQALLRMFLVPGLIAMPLIF
                     GWAAVTDLHYLEIGMFIAGFLTVAQFSFWGNYLPHAFPVHLRGTGEGFAANIGGRLIG
                     TCFFGVTQWIAFFLPATVGSYNTRVAYTAAGVAFTVYLINFLASFWLPEPDLKSLEE"
BASE COUNT         2095 a         3552 c         3401 g         2026 t
ORIGIN      
        1 cccgcaacag gcgggcgagc agcgtattgc ccgtaccgcg gagcgagccc gcgatcgtgg
       61 gcgaattcat catcgccagc gcctgcggaa tcgagctctg aatctcatct cgccgctggc
      121 tcgggtcgta gccgaaggcc gcggcgaatt gcccgcgcgg cccgccgccg aagccataca
      181 gcccgccgcg tccgccgccg aagccgcggg gctcgctggc ttcgagggcg acgagcaagt
      241 tgtcgaacag cacatcggcc cgcaggcgct gggccacgtt gtgctgcatc ggcggatctt
      301 ccgggccgcg ccgcggggcg ctcggcgttt gatacaaatc ggtcgccatg atcgtctgga
      361 aaagccactt cacgttgtag tcgctctcgg cgaattcctt cgccaggtat tccagcgtct
      421 gcggcgcgac agccgtccgg tccgggccca tgtcatcgac cggctcatag aagccctcgc
      481 cggtcagttc cgaccacatt cggttcacga aggcctgggc gaagtacggg ttgtccttag
      541 ccgtgatcca ctgggccagc gtgccgcggc ggtcggcgtc cttagttccc agcggcaact
      601 tctgacccgt cgcgaacagc acgggcgtca tcagcgtacc gcgcgactgc ggattcttca
      661 ggtccgacat gtagtgctcc ggcgtgccga accgccgcat gttcatcggc gctcggaagc
      721 ggccgccgaa ggccatgtcg ttggcggaca cgattacctg cggaccagtc tcttgattgt
      781 tgagaatgat gcggctggaa acacgcggga agaacgccgc cagttcgtga aactgctccc
      841 gcttccagcg gtcggttggg tgattgtggc actgggcaca cgaaatctgc acgccgagga
      901 aaatgcggct gacctcggaa gtcacctcct ccgggttgcc ttgttgcgca acaatcagcc
      961 cgcacgctcc gttttcggtc gcatcgcctt cggcggtgat gaactcggtt gcgatctggc
     1021 tccacggctt gtcggtattg aagctctcgg tcaggtaggt cgtcagcggg ccgttgatga
     1081 tccccgagcg gtcctcggtt ttgcgataca tgatcacgtc gcgccagtag cgtccccagt
     1141 tctcgccgaa ctcggcctgg gcgagcagct ttagggcgat tttcgcccgc ttgtcgctgc
     1201 tgggatcgag cgaaaaggcc gtcgtctctt cgggcgtcgg cagccggccg atgatgtcca
     1261 gggtggcccg ccgcaggaac cgctcgtcgt cgatcctccc cggcgaagcc tgccccgccc
     1321 ccgcccacgg caccgcggct cgcaagattt cattcgcctt ggcggcgacc gaagtcggct
     1381 cccccgcctg gcacggactg ggcctgaaca ccaccgcaat ggaggaggcg aatatgcata
     1441 ttaacaaaca atttcgaacg cacatgggac taatctccgc aagccgattg ctttgcaaac
     1501 cattacgaga caatcgtttg caacgatgcg tcgttgccca gcaatcgcaa ggtcgaatat
     1561 gcgtgtatgc gtcttatgtg tttccttccg cgaccgcggc aggcaggtga acaggtctat
     1621 aaaccgtcgc ctccagccga agttgcggcc caatcgccga acgctcaggc ggcggccatc
     1681 catgcatcgt cgcggagggg ccgaccaaac ggggcgcgca cctcgagcgc tcgctaaatg
     1741 aacttttgag ttctattcaa aagttcacta tccgtcaagg cgccttcggc tggttcttga
     1801 gctgccactt ctcatcgaaa aacccggcgt cgtgaatccg ccccggcgaa aaggcatccg
     1861 gcggctgggt gatatcaata atcgcccaat ccggcagctt cgggttctgc aacgaattcg
     1921 tccgatcgtg cccctcgcga aacgtgaggc cactgttgag gacgacgtag tgcggagcct
     1981 gacctgggcg aagacacggg tagatgcaca ccggtgcgaa gcggttccca tcaaatgtcg
     2041 aattgccgag cgaccatttt ccctccgcaa acttgatcgg cagcttctcc tccagatggc
     2101 ggatcaccga gttggaatcc gcgtcgcccc acagaatgat gttcttcagc ggcagaagtt
     2161 cggggtcaac gtcgcggtca tggacgacgg gcaattctcc gcgcatcagc gcccgccagc
     2221 gattctggaa gtggcgcagt tccacgtcgc accaggcctg aaaggcggga ttgcggctct
     2281 tgccggtggg agccacgacc atgaaggcct ccatgaatgc atcgtcaatc ggaccctgga
     2341 ggcttgggcg ctttgccagc gggctggaat acaaatctgc ttcctcggcc agcttccaag
     2401 tgtcgagcac tttcaccaat gtcgctgcat ccgaatcgcc gattctccga ttcacacgta
     2461 tttccgtgcc gtcaacgagg acacgcttcc catagaggtc catcggcggc gacagctcca
     2521 gcagcgacac attcttcgtc gtcacacgca ccaggtccgg cccgacgatc tccgcgtcca
     2581 cccggctgtc attccagtgt tcctccaagc gcagcgcttt cacccaacga tattggccgt
     2641 acttgagcgt ctgcgtttgc aggtgaactt cggtcggctt caattcgcgg cctttggtgg
     2701 ccgccgcctc caaccgcttc aacaactcgg ccagcgtctg cggctcatat ttgtgctcca
     2761 cgcccgggcc gatgaggtgc gtcagcgtcc gtccttccgc tagatacgct tcttccatga
     2821 cgcgagcggc ctgaatttgc ttgtcgttct cgccgctgta ggcgatgacc tgcgtgttga
     2881 acaggttgcg gacgtagccg ggaacgtcgt acagccccca taatttctgc tcgtaccagg
     2941 gcggatactt gtcgggcgtc agccgttgat agcgggccgt ttctgcgaat cccgctcccg
     3001 ggctgacggc gacgaactgg tccgcgtaat gagcgccaat gtgccacgct cccgcgcctc
     3061 ccatggaaaa gccgatcagc acgatgcgat cgttgtcgat acggtatcgc ttcttcacgc
     3121 ttgcgatggc ctcaaaaaca tcgatttctc ccgcatgctt ccagccgacg cagtgacggc
     3181 cgaaggggtg cagcacgatc gcgcccggcg gggcgatttg ccccggtttt gttgcccgct
     3241 cttgcaagaa gtgcaggtcg gtattcttgt cgccgcggcc gtgcagccag acgtagagcg
     3301 ggacgggttt gccgaaatcg tgatcggcgg gaatgacgag tccatacggc tgcgccgagc
     3361 cgtcgatctc cgagcggtag ccttgcacca cgaggccggg cgtgcgttcc caaggggctt
     3421 tcttatcggc gagttgatcc agccgcttgt tcgcttcgtc cagagcccaa tcggccttgg
     3481 cgaaatcctt ctccgcgtag aactcgcggt gcaagagcgc cagttcaacc gctttcgtga
     3541 atacctcgac gtccgcgcga tagacgctgt cttccgcgcc ctccagccgc ttctttgtcg
     3601 cggccaggcg cgcttccaga cgctggcgga tttcggcggg gatttcgagc ccttccggcg
     3661 gcaggacgcg ctggatcggc ggaatcggcg gcaggtcttc cgctgcggct ggagcgatgg
     3721 ggacgcaggc gagcaggcag accagtggga tcgtgcgggc gttcatggcg gcgaggcagg
     3781 agggaaaggg aaccgcggat cttgcggagg acgcggagta agcaagtcag acagcaaggc
     3841 ccagattagc gactgaccgg gcaaattgca cgtggcaagg gatccgcgaa tgaaacacgg
     3901 accggcacgg atggctccgg cgggtgcgga gttggtcgtc gaggagcgct tgagcccgcc
     3961 cgaatcttcg accgcgacga agcagttgtc ccgttgctca aacgacagcc cgcctgcggt
     4021 cagttgccgc gacagccact cgctgccgtt caggcagacg tgcagcgtga agggcagcca
     4081 agtctgcagg cgcagatgga ttcgaccaag ctgggggtct tggatgtaag aataatcgtg
     4141 ccggcacttg ctgggtccag accgcagctc cagacgcttc cgctcccggt tcggtcccac
     4201 gtggtacgag tagcatggct caacgcagcc caaggcgcag atcaatccgc tttagacctg
     4261 atccgtaatg gcaatctgct cgaccaattc ctcctcgcgg accgtgctgc tgttcaaata
     4321 gtgaaagggc cgcccggaag cgagcgccag gcactggctg ccgcggcgaa tccgcgtcgt
     4381 gcagtccacg gcaaattcct tgaagtcctt caagagcacg ctcacgcacg aaaggtaatt
     4441 gagcattccc cgcggattgg acagccaccg cagcgtcccc cgcagccgca cccgatcaaa
     4501 cccagtaccc cgctgatttg ctccgcatgg cgttgtataa acccttgcat ggagttcctc
     4561 cgaggatgaa gcattggctc tcgaaagctc caacacttta cacctgggag gaacgtcttt
     4621 gattaactca ccatccattc ggttgcggcg gagccgcgct gggtttgatc ggtgcaaatc
     4681 cgtggcttct ctcccgacct ccaaaccatg ccccgcgcta tgaatcctcc ttccgtgttt
     4741 ttcgtgtctt ccgtggtcct ctccctctgc cttgcagcaa acgccgcggg agagcagaag
     4801 cccaacatcg tcctgatttt cgccgatgac ctggggatca acgacctgtc gtgctatggc
     4861 cgccaggacc agcgcacgcc gcggctggac cggatggcgg cggaaggcgt gcggttcacc
     4921 tgcgcctact gctcgcagcc gatctgctcc ccctcgcggg ccggaatcct gaccggcaag
     4981 aatcctgctc ggctgcacat tacgaccttc ctgccgggcc gccccgacgc cccctcgcag
     5041 agactgctgc acccccagat cgccatgcag ctgccgctgg aagaaatgac gctggccgaa
     5101 tacctcaaag aagccggtta tgcgacggcc tgcatcggca agtggcacct gggcggcgcg
     5161 ggctttgggc cgaaggagca aggcttcgat tatgtccacg ccggccaggc caacaccaag
     5221 ccttccgcaa ccgaaggcgg caagggtgag tacgatctga cgaggcacgc cgtcgagttc
     5281 atcgagcaga acaaaaaccg gccgttcttc ctctatctgc cccacaacac gccgcacatt
     5341 ccccaagccg ccaagccgga gctggtcgaa aaatacaaag actcgttcaa tccggtcaac
     5401 gcggcgctca tggagacgct cgatgactgc gtgggcatcg tcctcgacaa gctcaaggag
     5461 ctgaagctcg acgaaaacac gatcgtcatt ttcacgagcg acaacggcgg cctgcacgtt
     5521 ttggagtttc ccggcgggcc atcgacgcac aacaccccct accgcggcgg caagggattc
     5581 ctctatgaag gcggcctccg cgagccggcc atcgtccgct ggccgggcaa gatcaagccg
     5641 gccgtgatcg atacgccgac cgtcaacctc gactgggcgc ccacgctgct gacgctcgcg
     5701 ggcctgaaga ttccagtggg aatcgatggc caggacattt cgcccctctt gctcggcgga
     5761 aaaatcgata tgccgccgcg agtcttctat tggcactttc cgcactacaa caaccaaggc
     5821 ggacgaccgg ctggcgccgt tcgcgagggc gattggaagt acatcctgtc gtacgacacg
     5881 ggccgcgtcg aactctacaa cctggcgacg gacgtggccg aggagcacga cgtcgcggtg
     5941 gacaacggcg ccgtgatcgc ccgcctcgaa gcgttgcgat cggactggct caagtccatc
     6001 gacggccaga acaatgcctc caatcccaac ttcgaccgca cgctcttcca caagctctac
     6061 gaagaattcg accccagccg gccgatcctc cgtaccaccg ccgccgggat ggaagaggat
     6121 atgaaggagt ggcgggcctt gatgaacgaa gtcgtccgcg gcaaccggcc ggcgaaggcc
     6181 aaagccaagc aacagtagcg cattgtttcg cgtctaccct ttcacagcgg cgggggccgt
     6241 gcgggaaacg gccggctgac gtacaatcgg cggcacgtga ccttcagccg tgaaccctgc
     6301 ccgcacatcg ttcatgcaag ttggcgtcct caaggagacc tttcccggcg agcggcgcgt
     6361 ggccctggtc ccggccgatc tgccgaagct ggccaaagcg ggaatgggag tgatcgtcga
     6421 gccgggcgcg ggagcggctg cggggttcgc cgacaaggcg tatgaggaga agggggccaa
     6481 attcgccttc aaccgcgccg gcgtcttcga agccgagatc gtcgtgcagg tccgctcgct
     6541 cggcgcgaac ctatcagccg gtcgcgccga tctgccactg ctgagatctg gccagattct
     6601 gctcggcacg gctgatccgt tgggggagcc gcaggcggtg gacgagatcg ccaagaccgg
     6661 cgcgacattc ttcgccctgg aattgattcc gcggacgacg cgggcccagg cgatggatgt
     6721 cctttcctcg caagccacca tcgccggcta tcgggccgtg ctcctggcgg caatagagct
     6781 gccgaagatc ttcccgctct tgatgacggc ggcagggacg ctcacagcgg cgaaggcgtt
     6841 tgtaatcggc gtgggcgttg ctggtttgca ggcgatcgcc acggcgaagc gactgggcgc
     6901 ggtggtgcgg gcctatgacg tgcgtcccgc gtgccgcgag caggtggaaa gcctgggggc
     6961 caagttcgtc gagctgccgc tggaatcggc agcggccgaa gtgcagggag gctacgccgc
     7021 ggtccaggac gagtcgtttt atcgccgcca gcgcgaactg ctctcgcaag tcgttgcgga
     7081 aagcgatgtg gtcattacga cagccgccat tcccggaaaa atgtcgcccc tgctcgtgcc
     7141 tgcggacgcg gtcgccaaaa tgcagcccgg cagcgtgatc gtcgatctgg cagcggagcg
     7201 cggcggcaac tgcgagctga cgaaggccaa cgagcgtatc gagatcaatg gcgtgacgat
     7261 cctcggtccg acgaacttgc cgagcgaagt gccaacacat gccagccaga tgttctcggc
     7321 caatgtgacg aatttcctct tgaacctggt gaagaagggg cagattgtgt tgaacctgga
     7381 cgacgaaatc atccgcgaaa cgctcgccgc cgaagggggc cagccggtgc atgcccgcgt
     7441 ccgcgaatcg ctcggcctgc ccaagctgcc cgagccggcg ccgctggaga cttaggggtc
     7501 aggggtcagg aatcagaaga cagaggacag ggtcaatgtt tgatcgcatc ctgcaaattt
     7561 ggcggtcctg cggtactgag tactcagtac taagtactca gtactatgtg cggcaagcgc
     7621 acgccgcacg aatcgccgtc ctgtgctttc tccctttgct gacggtcgct tgccagccag
     7681 agatggccca cgccgagccg ccaccggcgt ccgtcccatc gttggcgtcg gccgactcca
     7741 gtctcgcaca atccaccgcc gagaaggaac gcccgccgct cgtgctgagc ctgacgatct
     7801 tcgtcctggc gatcttcgtc ggctttgagc tgatcagcaa agtgccggcg accttgcaca
     7861 cgccgctgat gtcgggaagc aacgcgatca gtggcatcac ggtcgtcggc gcgatcatcg
     7921 ttgccggcgc aatcggtgtc gcgacacctt cgctcggctt cgtcgccaca gtactcgcca
     7981 tgatcaacgt tgtcggcggc ttcctggtca cccaccgcat gctccggatg ttcaaacgcc
     8041 gctaagttct gtcccctgcc ctctgtcccc agtcctcttg ccttgttcga cctcgcctac
     8101 ctcgccgccg ccctgctctt catcttcggc ttgaagaaca tggcccatcc gcgcaccgca
     8161 gtccgcggca atttactggg ggcgctcggc atggcggtgg cgatcgcggc tgccgtcgta
     8221 ttcgcggtac tcgatcccca aattaccgga tttagctacg gtgtgatcgg tgccgcgttg
     8281 gccttgggaa cggcgatcgg agccgtcctc gcgctgcgcg tgcagatgac tgccatgccg
     8341 caaatggtgg ccctgctcaa cggattcggc ggattggctt cggtgctcgt cgcgggagca
     8401 gatttgattc acgacccaca aaagcttgcc gcggacaaag ccggcctgga agttctcatc
     8461 gccgttgccg cctcggccat cgtcggcgcg gtcacgttct tcggcagctt gctggcctat
     8521 ggcaagctcg aagaattcaa gattctccgc cagccgatcc gctatccgct ccagcagccg
     8581 ctgaatgcgg tgctcgggct gatcctgctc ggcctttccg ccgcgatggt gtattacggc
     8641 ctgcaaggcg acgcggcgct aacggtcacg atgtttgtgc tggtgacgct cgtcgcttcg
     8701 gtcctcggcc tgacgctcgt caacccgatc ggcggggccg acatgccggt cgtgatttcg
     8761 ctgctcaatg cgtactccgg catcgccgcc ggcatgaccg gctttgtgat cgccaacaac
     8821 gtgctgatca tcagcggggc gctggtcggg gcgtcgggca tcatcctgac gcagatcatg
     8881 tgcaaggcga tgaaccgctc gctgctgaac gtcctgttcg gcacgctcac cgacagcggc
     8941 acaagcgcga aggccgggga catctatgcc agggtccgca gcacaacgcc cgaggatgtc
     9001 gccctgctcc tcgacggcgc ccaacgggtc gtcgtcgtgc ccggctacgg catggcggtc
     9061 gcccaggccc agcacgcggt ccgtgaactg ggtcaaacac tggaaaagcg gggcgtgacg
     9121 gtcgaatacg cgattcaccc cgtcgccggc cgcatgcccg gccacatgaa cgtgctctta
     9181 gccgaggcag acgtgcccta cgacaagctc ctggagatgg accagatcaa ccccacgctc
     9241 gacacggtcg atgtggcgat cgtcatcggg gccaacgatg tcgtgaatcc cgtcgccaag
     9301 accgacccca agagtcccat cgccggcatg ccgatcatcg aggtcgatcg ctgccgcacc
     9361 gtcgtcgtca tcaagcgcag cctcagtccc ggcttcgcgg gcatccccaa cccgctcttt
     9421 gctgccgaca acaccctcat gctcttcgcc gacggcaaag acgccgtgct ggagatcgtg
     9481 aaggcggtga aggaggggta gggagcagag gacagagggc aggaggcgag tcttattcct
     9541 ccagcgactt caggtccggc tccggcagcc agaagctggc gaggaaattg atcaaataaa
     9601 cggtgaaagc cacacccgcg gccgtgtacg ccacgcgggt gttgtagctg ccgacagtcg
     9661 ccggcaggaa gaacgcaatc cactgcgtca cgccgaaaaa gcacgtgcca atcagtcgcc
     9721 cgccgatatt cgcggcaaag ccttcgcccg tgccgcgcaa atgcacggga aaggcgtggg
     9781 gcagatagtt gccccaaaag ctgaactggg ccaccgtcag gaagcccgca atgaacatcc
     9841 cgatttccag atagtgcagg tccgtcaccg ccgcccagcc gaaaatcagc ggcatcgcaa
     9901 tcaggcccgg caccaggaac atccggagca gcgcctgccg gctgacgatg aacaagagca
     9961 ggattgcgat cgcgaagcgg ccaaccagcc cgccgatctc ctgcccttcg gtcgccttgg
    10021 tgatctttgg ctgttcgacc gggccggtaa tcgagcgcat tttgccgagg accgccttgt
    10081 cggctgcttc ctcggtcttg ccgtcggcaa tgagcttttt ccgcatccct tcctgagccg
    10141 ccttgggaaa ccccttctcc agtgcggcgt cagaggccgc acggacgtcg ggcagtcccg
    10201 gcacgatcag cggcatctgc tggatcgcgc cgaacgcggc ggcgaaactg caggtgaaca
    10261 tgagcgtggt gatcagcgtc gtgcgtcgga gtttgggtgc gaacaattcc gcgatgttcg
    10321 gccggcggag cgtgccggac tcgcgtttct tctgccaggc gggcgattcg ggcagaaacg
    10381 gccgaataac gatcagtgga atcgccggaa tcaagcccga catgagcagg tagcgccagt
    10441 cggcgtggcc gttcttgatc tcgcccagga aactcaagaa gttgggcaac tgcacttccg
    10501 gaagctgcaa cgcggggagc gtgatcaggc cgaacaggat cgtcggggat ttgttgaccg
    10561 cccaaccggc gacgatggtc cctgcgatag cgaccgacaa gccaccgatc gacgagaagg
    10621 cctgggtgaa tcccagcacg gcctcacggc gcttggggtt gtcgaacagt tccgccagcc
    10681 aggcgacggc ggccacgaat tcgacgcaga ccccgacaaa caccaggcaa cggaacaaga
    10741 gcagcatgta caggttcgtc gagaagcccg ccgcgaaagc agagccggca tacaggagga
    10801 tgctccaggt cagcacgcgc cgccttccca ggcggtcggt caagtagccg cccatcaggc
    10861 caaaaatgcc ccccgcgacg gcaggaatat agaacagcag gctgatccag gtcgtgaatt
    10921 cggtggtgcc cggacgaatg ccgccgagct cgagcagcgc tgggcgggca atcagcggca
    10981 gcatcagcag ctcgtagata tcgaatgcaa acccgatcgc cgcaataacg caaatcaacc
    11041 actgcgtgat cgtcagcggc ggggaactct ctag
//
LOCUS       DASUGB010000069         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1119589, whole genome shotgun sequence.
ACCESSION   DASUGB010000069 DASUGB010000000
VERSION     DASUGB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119589"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VFV87_01325"
     CDS             <1..1189
                     /locus_tag="VFV87_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX5102420.1"
                     /translation="FYNNHSFRGSIDISPDSLAGFTSDYNVVMNRFTTNGGDSIQTLS
                     QWQSSTGQDQHSLIATPSQLFVNPAGGDYHLSESSPAIDVGTSQYAPNFDFEGTTRPS
                     GNGIDIGADEREDAAPPPPPPSNQAPTDILLTNASVRENSADGTVVGLLSAVDPDSGD
                     THSFVLTNGAGSRFRIAGNQLMVRTGSLLDFEAATSHTIVVRTTDSGGLMFDKSFVIN
                     VQNVNELVGFDVQRGLAQRSYIRYIDLVFESADGLAELIAQGRFGLTRYNLNGANGVN
                     VSLTGKATASGNRIVIDFGAQGIGGKRNSATGDGYYALALDTDDDGSLETVRNFYRLL
                     GDSNGDRIVDATDVNNVNAAFGATGPHNADLNGDGVVNATDRNLANRRQGRKIASHLL
                     LHD"
     gene            1201..1941
                     /locus_tag="VFV87_01330"
     CDS             1201..1941
                     /locus_tag="VFV87_01330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5102421.1"
                     /translation="MTARPLSGKIALVTGAGRGIGRAIARAFAGEGATLVLAGRTAAH
                     LEEAAAEARSLSGQPAVVQPADVADEAQVDELFRRLKSEFGRLDLLANNAGAFDGGPL
                     DELSVEAWDKVINTNLRAPFLCTRAALRIMKPQKSGRIINIGSISAQRVRPNSGPYSA
                     SKHGLWGLTQVTALEGREFGISCCCLHPGNVMVERRVESGKLEDDEPMMSADEIAKVA
                     LLMATLPRHVEMLEAIVLPHQQLYVGRG"
     gene            1980..3035
                     /locus_tag="VFV87_01335"
     CDS             1980..3035
                     /locus_tag="VFV87_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5102422.1"
                     /translation="MLRLAFLGIDHPHGAHWRQLFASLGEQVELVALVPAFGGGVASL
                     EERYADLPRFESARQLVEWGQFDAAMVAMSNREGPDAIVELAKAGKHVLAEKPVAAGA
                     ADGRRIVDAVEKGGVAFQTGYMWRYDEIANRLKRMMQQGAFGKLISVEMTFVTSDVTR
                     RGGDHYLFDPAQSTDGFFSWLACHWLDTLFYVTGRAVVGVTARAGVFGAVPTQVEDGG
                     VAIFDLEGGGIATFLGGYWLPRWAGESKWAIRGSQRWVHWDANRPGTSGVLDIHGPKP
                     QWHAMEETFTGPDDKTPGYGGLRGRLLVQDWLDCIQTGRRDNRNTARPMLAVLELIDA
                     ILQSSRERRRIDCRIGP"
     gene            3199..>3805
                     /locus_tag="VFV87_01340"
     CDS             3199..>3805
                     /locus_tag="VFV87_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102423.1"
                     /translation="MYRFAFASLVLLSNAAVADDWPQFRGPQAGVAVGDQALPAEIGP
                     EENVLWKTELPPGHSSPAIVGNKIFLTAVRDNEHLETICLDRPSGKILWRVEAPHKTL
                     EQIHGIGSYAQPSPAADKERVVVLFGSSGLYCYDHGGKELWHKEMGPFNDEFGAAASP
                     IIEGERVILGQDHDTGSFLAAYDKRTGEERWRTDRSEFSRNY"
BASE COUNT          705 a         1198 c         1218 g          684 t
ORIGIN      
        1 cttctataac aaccactcgt tccgcgggag catcgacatc agccccgaca gcctggccgg
       61 ctttaccagc gattacaacg tggtaatgaa tcgcttcaca accaacgggg gcgattcgat
      121 ccaaacgctg tcccagtggc agagctcgac cgggcaggac cagcattcgc tcattgccac
      181 gcccagccag ctcttcgtca atccggccgg cggcgactat cacctttccg agtcgagccc
      241 ggcgatcgac gtcggcacat cgcagtacgc gccgaatttc gatttcgaag gcacgacccg
      301 gccgagtggc aacggcatcg acatcggcgc ggacgagcgc gaagacgcgg ctccgccgcc
      361 gccccctccg agtaatcaag ccccgaccga tatcctcctc actaacgcca gcgttcgcga
      421 aaacagcgcc gatggaaccg tcgtcggctt gctctccgcc gttgaccccg actccggcga
      481 tacgcactcg ttcgtgctta cgaacggcgc cggcagccgt tttcgcattg ccggcaacca
      541 actcatggtc cgcaccggca gcctgctcga ctttgaggct gcaaccagcc atacgatcgt
      601 cgtccggaca accgacagcg gcggactgat gtttgacaag tcgttcgtca tcaacgtgca
      661 aaacgtcaat gagctcgtcg gtttcgacgt gcagcgtggg ctggcgcagc ggtcgtatat
      721 tcgctacatc gacttggtct tcgagagtgc agatgggctg gccgagctta ttgcccaagg
      781 gcgatttgga ttgacccgct acaacctgaa cggggccaat ggcgttaacg tcagcctgac
      841 gggcaaggcg acggccagcg gaaaccgcat cgtcatcgat tttggtgctc agggcatcgg
      901 cggcaagcgc aactccgcca cgggcgacgg ctactacgcc ctggcgctgg atacggacga
      961 cgacggcagc ttggagactg tccgcaactt ttatcgtctg ctcggcgaca gcaacggcga
     1021 ccgcattgtg gacgccacgg acgtcaacaa cgtcaacgcc gcctttggtg cgaccggccc
     1081 gcacaatgcc gatctgaacg gcgacggcgt cgtcaacgcg accgaccgca accttgctaa
     1141 ccgccggcaa gggcgtaaga tagcgtccca tctgctattg cacgattgaa cgctctcgcc
     1201 atgaccgcgc ggcccttgtc cggaaaaatc gcactcgtta ccggcgccgg ccgcgggatt
     1261 gggcgagcca ttgcgcgggc cttcgccggc gaaggggcga cgctggttct tgccggacgg
     1321 acggcggcgc atctggaaga agccgctgcc gaggctcgtt cgctcagcgg gcaacctgcg
     1381 gtcgttcagc cggcagacgt tgccgatgaa gcgcaggttg atgaactgtt ccgaaggctg
     1441 aagagcgagt ttggccggct cgatttgctg gcgaacaacg ccggggcatt cgacggcgga
     1501 ccgctggacg agctctccgt cgaagcctgg gacaaggtga tcaacacgaa tctccgcgcc
     1561 ccgttcctct gcacccgtgc cgcgctgcgg atcatgaagc cgcagaagtc cggccggatc
     1621 atcaacatcg gctcgatctc ggcccagcgg gtgcggccga attccgggcc gtattcggcc
     1681 agcaaacacg gcctgtgggg gttgacgcag gtgacggccc tggagggtcg tgagttcggc
     1741 atcagctgct gctgtctgca cccgggcaat gtgatggtcg agcggcgtgt ggagagcggc
     1801 aagctcgagg acgacgaacc gatgatgtcg gccgatgaga tcgcaaaagt tgcgctgctc
     1861 atggccacac tcccgcgcca cgtcgaaatg ctcgaagcga tcgtcctccc gcaccagcag
     1921 ctttacgtcg gtcgcggctg actgtcccct gtttcctatc gtctgtctcc tcccgcccca
     1981 tgctccgcct cgccttcctc ggcatcgatc acccgcacgg cgctcactgg cggcagcttt
     2041 ttgcgtcgtt gggcgagcag gtcgaactcg tcgcgctggt gccggcgttt ggcggcggag
     2101 tggcgagcct ggaagaacgc tacgccgatc tgcctcggtt tgaatcggct cggcagctcg
     2161 tcgagtgggg gcaattcgac gcggcgatgg tcgcgatgtc gaatcgcgaa ggcccggacg
     2221 cgatcgttga gctggcgaaa gccggcaagc acgttctggc cgaaaagccg gtagcggctg
     2281 gggcggcgga tggccggcgg atcgtcgacg ccgtcgagaa aggcggcgtc gcctttcaga
     2341 ccggctacat gtggcgatac gacgaaatcg ctaaccggct gaagcggatg atgcagcaag
     2401 gagcgttcgg caagctgatc agcgtggaga tgacgttcgt caccagcgac gtgacgcgcc
     2461 gcggcggcga tcactatctg ttcgatccgg cccaaagcac cgacggcttt ttcagttggc
     2521 tggcctgcca ctggctcgac acgctgttct acgtcaccgg ccgggccgtc gtcggcgtga
     2581 cggcgcgggc cggcgtcttc ggggccgtgc cgacgcaggt cgaagacgga ggcgtggcga
     2641 ttttcgacct ggaagggggc ggcatcgcca cgttcctcgg cggttattgg ctgcccaggt
     2701 gggccggcga gagcaagtgg gccattcgcg gcagccagcg gtgggtgcac tgggacgcca
     2761 accgtcccgg gacgagcggc gttctcgaca ttcatgggcc taagccgcag tggcatgcaa
     2821 tggaagaaac cttcaccggc ccggatgaca aaactcctgg ctacggcggc ctccgcgggc
     2881 gattgctcgt gcaggactgg ctcgactgca tccaaaccgg ccgccgcgac aaccgcaaca
     2941 ccgcccgccc catgctggcc gtcctcgaac tcattgacgc gatcctccaa agcagccgcg
     3001 agcgccggcg gatcgactgc cggatcgggc cttagcggat gatcggacgt gtaggataca
     3061 atcacgagga cacaaagttc acgaaggagg atgggcaggc aaacggatgg aattactaca
     3121 tctcctctgg ttaaaattct cacttctccc tttgtgccgt tcgtgtcttt gtgtttccct
     3181 cctccattcc tgcccaccat gtatcgattt gcctttgctt cactcgtgtt gctgtccaac
     3241 gcagccgttg ccgatgactg gccccaattt cgcgggcccc aagctggcgt agccgtgggc
     3301 gaccaggcgc tgccggcgga gatcgggccg gaggagaacg tcctctggaa gacggagttg
     3361 ccgccggggc attcgtcgcc cgcgattgtc gggaataaga tcttcctgac ggccgtgcgg
     3421 gacaacgagc atttggagac catttgtctc gatcggccga gcggcaagat cctctggcgc
     3481 gtggaggctc cgcacaaaac gctggagcaa atccacggca tcggcagcta cgcccagccg
     3541 agcccggcag ccgataagga gcgcgttgtc gtgctgttcg gctccagcgg gctgtattgc
     3601 tacgaccacg gcggcaaaga gttgtggcac aaggagatgg ggccgttcaa tgatgagttt
     3661 ggcgcggccg cttcgccgat catcgaaggc gagcgcgtga tcctgggcca ggaccacgat
     3721 acgggctcat tcctggcggc atacgacaag cggacgggcg aggagcggtg gcggaccgac
     3781 cgctcggaat tctcgcggaa ttatt
//
LOCUS       DASUGB010000070         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_286265, whole genome shotgun sequence.
ACCESSION   DASUGB010000070 DASUGB010000000
VERSION     DASUGB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286265"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..439)
                     /locus_tag="VFV87_01345"
     CDS             complement(11..439)
                     /locus_tag="VFV87_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX5102424.1"
                     /translation="MSVNVLYKTSAKATGGRDGTAATLDGALDVKLATPKELGGGGGA
                     GNNPEQLFAAGYAACFIGAMKAVAAQGGGVKVPADASVTSTVGIGPRSEGGFGLDIDL
                     AVSLPGVPKADAEALVQKAHHVCPYSNATRGNVDVRLTVV"
     gene            528..1058
                     /locus_tag="VFV87_01350"
     CDS             528..1058
                     /locus_tag="VFV87_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5102425.1"
                     /translation="MYAIEIRYLGSQISVWILRANEVVMARKSSADFPLRLDNQICFA
                     VYSAAHAFNRVYKPLLDKLGLTYPQYLVMLVLWERDGVPVKDIGERLFLDSGTLTPLL
                     KRLETAELIKRTRSTEDERQVLIALTSKGESLREKARAVPQSILAASACSVGELVGMK
                     NEIVALRDRLNAVLGE"
     gene            complement(1154..1229)
                     /locus_tag="VFV87_01355"
     tRNA            complement(1154..1229)
                     /locus_tag="VFV87_01355"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1194..1196),aa:Ala,seq:ggc)
     gene            complement(1294..1713)
                     /locus_tag="VFV87_01360"
     CDS             complement(1294..1713)
                     /locus_tag="VFV87_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX5102426.1"
                     /translation="MAKSDLGTKRICPTTGKKFYDLNKNPVISPYTGEVVPIAPIAPP
                     RAARGEAARAAAASAAASDMPEAAEAEELVSLEEADAEENTGKVKAQVPESEDDIEID
                     ETIEDDDDDDSTFIADEEEGDEDVTDIIGDVGGDEET"
     gene            1857..>2580
                     /locus_tag="VFV87_01365"
     CDS             1857..>2580
                     /locus_tag="VFV87_01365"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5102427.1"
                     /translation="MTDSPAQTPLESRASGPLSGKVRVPGDKSISHRALILGALSVGE
                     TRISGLLEGEDVVNTAKSMQALGASVERTGPFAWKVAGVGVAGFAQPSAPLDFGNSGT
                     GCRLVMGAVAGCPITAVFDGDASLRSRPMRRILDPLELMGARAGEAREGGRLPLTLHG
                     ARDPVPILYRTPVASAQIKSAVLLAGLAAPGVTTVIEQEASRDHTELMLKHFGAGIVS
                     AKEGSHGRRIALTGQPELHGAEV"
BASE COUNT          450 a          807 c          835 g          488 t
ORIGIN      
        1 cgctcgtggg tcagacgacg gtcaggcgga cgtcgacatt gccgcgggtg gcgttggaat
       61 aggggcaaac atggtgggct ttctgcacca gcgcttcggc atcagccttg ggaacgccgg
      121 gcagcgaaac ggcaagatcg atgtcgaggc caaacccgcc ttccgagcgc ggaccgatgc
      181 cgaccgtgga ggtcacggac gcatccgcgg gcactttcac gccaccaccc tgggcggcga
      241 ccgccttcat cgcaccgatg aagcaggcgg catagccggc ggcaaacagt tgttcgggat
      301 tgttgccggc accgccgccg ccaccgagtt ccttcggggt ggcgagcttg acgtcgagcg
      361 cgccgtcgag ggtcgcggcg gtgccgtcgc ggccaccggt ggccttggcg ctggtcttgt
      421 agagcacgtt cacggacatg tcggtctccc ctgccgtttc gacggaccat gtattgcaca
      481 caattatatt gtgcgcaatc aaaaatattg tgggcagcac aatttaattg tatgcaattg
      541 aaatcaggta cttaggctct caaataagcg tctggatact acgcgctaac gaggtcgtga
      601 tggccaggaa atcttcggcg gactttccgc tgcggctcga caatcagatt tgcttcgcgg
      661 tgtattccgc cgcgcatgca ttcaaccgcg tctacaagcc gctgctcgac aagctcggcc
      721 tgacctaccc gcaatatctc gtcatgctgg tgctgtggga gcgcgacggc gtgccggtga
      781 aggacatcgg cgagcgcttg ttcctggatt caggcacgct gacgccgctg ctcaagcgac
      841 tggagacagc cgaactgatc aagcgaacac gcagcacgga agacgagcgg caggtgctga
      901 tcgcgctcac gtccaagggc gaatcgctca gggagaaagc gcgggccgtg ccgcaatcga
      961 tcctcgcggc gtcagcctgc tccgtcggcg aactcgtggg gatgaagaac gagatcgtgg
     1021 cgctgcgcga tcggttgaat gcggtgctgg gggagtaggg gcgagcgctt gggatgcttc
     1081 tcgacgctag ggctcgccga ggcccgccgt cgcccttcgg gctatggcgg ggcagccttc
     1141 gctacgcgaa ggctggtgga gccaggcggg atcgaaccgc cgacctcttg catgccatgc
     1201 aagcgctctc ccagctgagc tatggcccct tagaatcgcg cgcctgaaat tcagcgtgcg
     1261 ggcatgaccc tgaaccacag ttctggatcg atctcaagtc tcttcgtcac ctccgacatc
     1321 accgatgatg tccgtgacgt cttcatcgcc ctcttcctcg tcggcaataa aggtggaatc
     1381 gtcgtcgtca tcgtcctcga tggtctcatc gatttcgata tcgtcttccg attcgggaac
     1441 ctgggccttg accttgccgg tattctcctc ggcatcggcc tcttcgagcg aaaccaactc
     1501 ttcggcctcc gcggcctccg gcatgtcgga agcagcagct gaagcggcgg cggcgcgtgc
     1561 agcttcgcca cgggcggccc gcggcggcgc aatcggcgcg atcggcacga cttcgcccgt
     1621 atagggcgag atcaccgggt tcttgttcag atcgtagaat ttcttacccg tcgtcgggca
     1681 aatgcgcttg gttccgaggt cggatttggc cacgtgtgga tcctgggaaa ttctgaaaaa
     1741 cggtgcttca cttggctagt tgagaggctg gtgtcaatag cggtttgtcg catttgactg
     1801 ccgcgtgacg tgatggggcc catgtggtac tgccgccccc gcagaggata cccatcttga
     1861 ccgattcacc cgcccaaacc ccgctggaat cccgcgcaag cggcccgctg agcggcaaag
     1921 tccgcgttcc cggggacaag tcgatttcgc accgtgccct cattctcggg gcgctttcgg
     1981 tcggagaaac ccggatttcc ggcctgctgg agggcgaaga cgtcgttaat accgccaaat
     2041 ctatgcaggc gctgggcgcc agtgtcgagc ggaccgggcc gtttgcctgg aaggtcgccg
     2101 gcgtcggcgt ggccggcttc gcccagccct ccgctccgct cgatttcggc aattccggca
     2161 ccgggtgccg gctggtaatg ggggcggtcg ccgggtgccc gatcacggca gtattcgacg
     2221 gcgatgcctc gctgcggtcc cgcccgatgc ggcgaatcct cgatccactc gaattgatgg
     2281 gcgccagggc cggcgaggcc agggaaggcg gccgcctgcc actgacgctg catggcgccc
     2341 gcgacccggt gccgatcctc taccgcacac cggtggcctc ggcccagatc aagtcggcgg
     2401 tgctgctggc cggacttgcc gcgccgggcg tcacgaccgt catcgagcag gaagccagcc
     2461 gcgaccacac cgaactgatg ctgaagcatt ttggcgccgg gatcgtctcg gccaaagagg
     2521 gcagccacgg ccgcagaatc gcgctcacgg gccagcccga actgcacggc gccgaggtgg
//
LOCUS       DASUGB010000071         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1397682, whole genome shotgun sequence.
ACCESSION   DASUGB010000071 DASUGB010000000
VERSION     DASUGB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397682"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFV87_01370"
     CDS             complement(<1..197)
                     /locus_tag="VFV87_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102428.1"
                     /translation="MLVPRLRLGTQVPEALPRCVAWGLCATAVLLAAGLPAAEGPKPQ
                     VATSKTGLVASVHPLATDAGVA"
     gene            complement(229..1272)
                     /locus_tag="VFV87_01375"
     CDS             complement(229..1272)
                     /locus_tag="VFV87_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX5102429.1"
                     /translation="MSQRNHSCPDCEGRGFELDRRHFLAATGAVATAAVLPRVVQADD
                     KADKPAPETLVKTLYGTLTDEQKKTVAFDWDYEHPQWGLLRTRVSNNWHITRPEIAGD
                     FYTKDQQEICRAIYEGLFQPEWVKKIDQQLQDDNGGKVWGAAQNVAIFGKPGENKFEL
                     VMTGRHMTVRVDGNSVDHMAFGGPTFHGHAASGFNEKVGHPDNIWWPQALAANEVFKM
                     LDGKQREKALVAQMPAEQSVGFRGADGQFQGIPIADLTADQKELVQKTLQKVLEPYRN
                     SDRDEALDCLKKQGGLDKCSLAFYAAGDLGDDGEWDNLRLEGPSFVWYFRGTPHVHMW
                     TNIGADAAVKLNA"
     gene            complement(1303..>2087)
                     /locus_tag="VFV87_01380"
     CDS             complement(1303..>2087)
                     /locus_tag="VFV87_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102430.1"
                     /translation="KAGISVWYRHGARRNEQSTPGFNQWSFQRGIAGMVRSPFVVGLL
                     LLAPGGFVRSEENAAPAVEALRSALVKNIEHSRDWLAAKDFKSLGQSAGGLELLAEVL
                     RAKSDDAAWQAATGQVLAAAKEVRAAAQAEDGPRSAEALAALEKKAADAAGISPAGQP
                     LPPPRANLRSLMLLMDGVYADGKIALLTDQPQFAKNQAQVLSELGRVVSNSRSGDRWA
                     GLSDGFVQAAQNAAQSSEADAQALRPLMRAISQRCEACHDTR"
BASE COUNT          352 a          661 c          662 g          412 t
ORIGIN      
        1 gccacgccgg catcggtcgc cagtgggtgg acggaggcga cgaggccggt cttcgaggtg
       61 gctacttggg gcttcggtcc ttcggctgcg ggcaggccgg ccgcgagcag gactgctgtc
      121 gcgcagagcc cccacgccac gcagcgaggc agagcctcgg ggacttgcgt tcccaggcgg
      181 agcctgggaa cgagcacctg acacctgatc cctgactcct gaccgctgct aggcattcag
      241 cttcaccgca gcgtcggcgc cgatgttcgt ccacatgtgt acgtggggcg tgccgcggaa
      301 gtaccagacg aaggacgggc cctccagccg caggttgtcc cactcgccgt cgtcgccgag
      361 gtcgccggca gcgtaaaagg cgagcgagca cttgtcgagg ccgccctgct tcttaaggca
      421 gtcgagggcc tcgtcgcggt cggaattgcg gtagggctcc aaaaccttct gcagcgtctt
      481 ttgcaccagt tccttctgat cggcggtcaa gtcggcgatg ggaatgccct ggaactggcc
      541 gtccgcgccg cggaagccga ccgattgttc ggcaggcatc tgagcgacga gcgccttttc
      601 gcgctgcttg ccgtcgagca tcttgaaaac ttcgttcgcg gccagagcct gcggccacca
      661 gatgttgtcc ggatggccga ccttctcgtt gaagccgctg gcggcatgac cgtggaatgt
      721 cggcccccca aaggccatgt gatcgacact gttgccatcg acgcggactg tcatgtgccg
      781 gccggtcatg accagctcga atttattttc gcccggcttg ccgaagatgg cgacgttctg
      841 ggcggcgccc cagaccttgc cgccgttgtc gtcctggagt tgctggtcga tctttttgac
      901 ccattcgggc tggaagaggc cctcgtaaat cgcccggcag atttcctgct ggtccttggt
      961 ataaaaatcg ccggcgattt cgggccgcgt gatgtgccag ttgttcgaga cgcgggtgcg
     1021 gagcaggccc cactgcggat gctcgtagtc ccaatcgaag gcgaccgttt tcttctgctc
     1081 gtcggtgagc gtgccataga gcgttttgac gagcgtttcc ggcgcgggct tgtcagcctt
     1141 gtcgtcggct tgcacgacgc gtggcagaac ggcggcggtt gccaccgcgc ccgtggcggc
     1201 caaaaagtgt cgccggtcga gttcaaagcc gcgaccttcg caatccgggc aagaatggtt
     1261 ccgctgagac atgagaacgc tcctggagat gagatgaatg acttacctgg tatcgtgaca
     1321 agcttcgcac cgctgcgaaa tggctcgcat cagggggcgc aacgcttgag catcggcttc
     1381 gctcgactgg gcggcattct gcgcggcctg aacgaaaccg tcgctgaggc cggcccaccg
     1441 gtcgccgctt cgtgaattag aaaccacccg ccctagctcc gacaatactt gggcctgatt
     1501 tttcgcgaat tggggctggt cggtcaggag ggcgattttg ccgtccgcat agacgccgtc
     1561 catcaacagc atgagcgagc ggagattggc ccgcggcggc ggaagtggct ggccggcagg
     1621 tgaaatgccg gcggcatctg cggccttttt ttccaatgca gccagggcct ctgcggatcg
     1681 cgggccatcc tcggcctgag cagccgctcg tacctcttta gccgccgcca gaacctgccc
     1741 ggtcgccgct tgccaggccg catcgtcact tttggcccgg agcacctccg ccagcaactc
     1801 taacccgcct gccgactgcc ccagcgactt gaaatccttg gcagccagcc agtcgcggga
     1861 atgttcaatg ttcttgacca aagccgaccg gagcgcttcg accgctggtg cggcgttttc
     1921 ttcggagcgc acgaagccgc cgggagccag caagagcaag ccgacgacaa acggtgagcg
     1981 caccattccc gcgattcccc tttgaaaact ccactgattg aaccccggag tggattgctc
     2041 gtttcgccgc gcgccgtgac ggtaccaaac gcttattcca gcttttc
//
LOCUS       DASUGB010000072         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_564685, whole genome shotgun sequence.
ACCESSION   DASUGB010000072 DASUGB010000000
VERSION     DASUGB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564685"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFV87_01385"
     CDS             complement(<1..526)
                     /locus_tag="VFV87_01385"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX5102431.1"
                     /translation="MTPLAQLDPAAMALALARELARGLPGRAAQRVMAHRLAYGRHHG
                     PIPEGARRAAVLLALHQSPAGWSVPAVLRPPTMRSHAGQVSLPGGSTDADETAVQTAL
                     REMEEELGVPASELTVIGQLTPVYVYVSGFEISPVLAVAKEPLAYRPNPHEVAAVLEL
                     PLAQLCDPACRGEHP"
     gene            complement(640..2349)
                     /locus_tag="VFV87_01390"
     CDS             complement(640..2349)
                     /locus_tag="VFV87_01390"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HEX5102432.1"
                     /translation="MTDSLASKKRPASAPAARASGERIGPGWTAFEIVWIFLIFCLMA
                     MSPTPDAGESAYLVKAKHYWDQAWCAGDLFLESADTHLGFYWTFGWLTRYSSLDAATW
                     MLRFVAWGLLAWAWQRLSAAIVPRPLFSLLSAGLMLLFAHQMHWAREVILRGGVEGKT
                     FAYVAVLLALGEIVRTKWSLALLLAGVAGMFHVLVGGWCAIAIGLAWLAAGSERPRLR
                     SLIPGIVGGLMFASLGLVPTLALILGPQPANAAEAARTYVFDRLAHHLVFHRFGTWYH
                     VRHAVLLAAWGAAFWLTRKEPGLFRLQLVVGGTVLIALVGIVLDQALVIRANLLGQSL
                     EEYQLAAAPWLKFYWFRVEDVLVPIGVALAATLGISRVQVRRPELGNWLLIGAMLAAA
                     ANVVDSIYWRNRLGVPGAVIQQQPTADTRLTWWWESAPETLTAREWQAAWRNVCQWIG
                     ENTPPEARFLTPRNQQTFKWHAGRTEVATRKDIPQDAASILAWRAALDELYPNDMPHR
                     RHDLAEFSDEQLIALARKYDCQYIVIDQSRAGRRIGLPQVYPIVREDNRLFAVYRVPL
                     AGR"
     gene            2417..3304
                     /gene="prpB"
                     /locus_tag="VFV87_01395"
     CDS             2417..3304
                     /gene="prpB"
                     /locus_tag="VFV87_01395"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEX5102433.1"
                     /translation="MSPGQELRELVKDATIQVPGATSALVARMIERAGFDALYLSGAA
                     FSAGVLGLPDVGLFTLTELAQQTAYLTKASSLPLIVDADTGFGEALNVERTVIELEAA
                     GAAAIQLEDQQLPKRCGHLAGKSLVEPAVMCAKLRAACAAREDTSLVIIARTDARGVT
                     DINDAIERAKRYLDAGADWIFPEALQSKAEFDQFSDAIDAPLVANMTEFGQSPLLTLE
                     ELAELGYSAVLYPVTLLRVAMKATEAALNFLAADGTQQELLDLMQTRDELYDLLDYKH
                     FEERDRAHANDQEPAIKNQ"
BASE COUNT          615 a         1194 c         1099 g          490 t
ORIGIN      
        1 tcgggtgctc gccccggcaa gctgggtcgc aaagctgggc caggggcagc tcgaggaccg
       61 cggccacctc gtgcgggttc gggcgatagg ccagcggctc tttggcgacc gccagcacgg
      121 gcgaaatctc gaagccgctg acatagacat agacgggcgt gagctggccg atgaccgtca
      181 gctcgctcgc cggcaccccc agctcttctt ccatctcgcg gagggccgtc tgcaccgctg
      241 tctcgtccgc gtcggtcgag ccgcctggca ggctgacctg tccggcgtgc gagcgcatcg
      301 tcggcggccg caacaccgcg ggaaccgacc agccggcggg cgattggtgc agcgccagca
      361 gcacggccgc ccggcgggcg ccctcgggaa tcggcccatg atggcggcca tacgccaggc
      421 gatgcgccat cacccgctga gccgcccgac ccggcaagcc tcgcgccaac tcgcgcgcca
      481 gggccagcgc catcgcagcg ggatcgagtt gagcgagcgg ggtcatcaat ggtggtcgcg
      541 ccggcggaaa gggcggcgca aaggggtcgg gagtcaattg cggtcacggc gctccatctg
      601 gatgatgtct gccgcaattg acgcccgacc ccctcaaggt tacctgcccg ccagcggcac
      661 gcgatagacc gcaaacagcc ggttgtcctc gcggacgatc ggatagacct gcggcaggcc
      721 gatccgccgg ccggcgcgcg actggtcgat cacgatgtac tggcaatcgt acttgcgggc
      781 cagggcgatg agctgctcgt cgctgaattc cgccaggtcg tgccgccggt gcggcatgtc
      841 gttgggatag agctcgtcga gggccgcccg ccacgcgagg atgctcgcgg catcttgcgg
      901 gatgtccttc cgcgtggcca cttccgtccg gcccgcgtgc catttgaatg tctgctggtt
      961 gcggggcgtg aggaagcggg cctccggcgg cgtgttctcg ccgatccact ggcagacgtt
     1021 ccgccaggcg gcttgccact cgcgagcggt gagcgtctca ggcgccgatt cccaccacca
     1081 tgtcagccgc gtgtcggccg tcggctgttg ctggatcacc gcgccgggca cacccaggcg
     1141 attgcgccag tagatggaat cgaccacgtt cgccgccgcg gccagcatcg ccccgatcag
     1201 cagccagttg cccaattccg gccgccgcac ctgcacgcgg ctgatgccga gggttgccgc
     1261 cagcgccacg ccgatgggca ccagcacgtc ctccacgcgg aaccaataaa acttcagcca
     1321 cggtgccgcc gcgagctgat attcctccag cgattgcccg agcaggttcg cgcggatcac
     1381 cagcgcctga tcgagcacga tgccgaccag cgcgatcagc accgtcccgc cgacgacgag
     1441 ctgcaagcgg aagaggcccg gctccttgcg cgtgagccaa aacgccgccc cccacgccgc
     1501 gagcagcacc gcgtgccgca cgtgatacca cgtgccgaag cggtgaaata cgaggtgatg
     1561 cgcgaggcgg tcgaagacgt aggtccgcgc cgcctcggcg gcattcgcgg gctgcggccc
     1621 gaggatcaac gcaagcgtgg gtacgagtcc gagcgaagca aacatcaggc cgccgacgat
     1681 tccgggaatc aacgagcgca gacgaggccg ctcgctgcct gcagcgagcc acgcgaggcc
     1741 aatcgcgatc gcgcaccagc cgccgacgag cacgtgaaac attcccgcga cacccgcgag
     1801 aagcagcgcc aacgaccact tggtgcgcac gatctctccg agtgccagca gcacggcgac
     1861 atacgcaaac gtcttccctt ccacgccgcc gcgcaggatc acttcccgcg cccagtgcat
     1921 ctggtgggca aacagcagca tcagtccggc cgatagcagc gaaaagagcg gccgcggcac
     1981 aatcgccgcg ctcaagcgct gccacgccca cgccagcaag ccccaagcca caaagcgcag
     2041 catccacgtc gccgcatcga gcgaggaata gcgcgtcagc cagccgaacg tccaatagaa
     2101 ccccaggtgc gtgtcggccg attcgaggaa caggtccccc gcgcaccagg cttgatccca
     2161 atagtgcttc gccttgacca ggtaagccga ctcgcccgcg tccggcgtcg gcgacatcgc
     2221 catcaggcag aagatcaaaa agatccagac gatctcgaac gccgtccagc ccgggccgat
     2281 tcgctcgccg ctggcgcgcg cggcaggcgc ggacgcgggt cgcttctttg aagctaacga
     2341 atcggtcatc gagccagact acttgcgccc gcccattctg gccagtcccg cggcaagggc
     2401 ctatcatgcg ggaaccatgt cccccggcca ggaattacgc gagctggtaa aggacgcgac
     2461 cattcaggtc ccgggagcga ccagcgcgct cgtcgcccgg atgatcgagc gggccgggtt
     2521 cgacgcgctg tacctttcgg gcgcggcgtt ctccgccggc gtgctcggcc tgcccgacgt
     2581 gggcctgttc acgctcaccg agctcgccca gcagaccgcc tatctgacca aggccagctc
     2641 gctgccgctg atcgtcgatg ccgacaccgg ctttggcgag gcgctgaacg tcgagcgaac
     2701 cgtcatcgag ctcgaagcgg cgggcgccgc ggcgatccag ctcgaagacc agcagcttcc
     2761 caagcggtgc gggcacctgg cgggcaagtc gctcgtcgag ccggcggtga tgtgcgccaa
     2821 gctgcgggcg gcttgtgccg cccgcgagga cacgagcctg gtgatcatcg cccgcaccga
     2881 cgcccgcggc gtgacggata tcaacgacgc gatcgagcgg gccaaacgct atctcgatgc
     2941 cggcgccgac tggatcttcc ccgaggccct gcagagcaag gccgagttcg accagttttc
     3001 ggacgcgatt gacgccccgc tcgtcgcgaa catgaccgag ttcggccaaa gcccgctgct
     3061 cacgctcgag gagctggccg agctgggtta ttccgccgtg ctgtatcccg tgacgctgct
     3121 gcgggtcgcg atgaaggcga ccgaagccgc gctgaatttc ctggccgccg acggcacgca
     3181 gcaggaactg ctcgacctga tgcagacccg cgacgaactg tacgacttgc tcgactacaa
     3241 acacttcgaa gagcgcgacc gcgcgcatgc gaatgaccaa gaaccagcaa tcaagaacca
     3301 atgaccaaag ttccacggtc gcagtgtgaa agccccaccg gccctgtgcc ggtggtaccg
     3361 tgattggcag ctagatattc acccgcgcgc tttgtcgt
//
LOCUS       DASUGB010000073         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1955007, whole genome shotgun sequence.
ACCESSION   DASUGB010000073 DASUGB010000000
VERSION     DASUGB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955007"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1014)
                     /locus_tag="VFV87_01400"
     CDS             complement(238..1014)
                     /locus_tag="VFV87_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HEX5102434.1"
                     /translation="MSTNPVALVTGASRGIGRAIAVRLAREGYAVGVNYHSNANAAAE
                     VVKEIEQAGGRALPFQADVAQAADRQGMAADLLDRFGRLDVLVNNAGITSIGRKDLLE
                     ATEESWDAVFATNLKGPFFLAQLAARKMIGLIEAQAISRGTIVNVSSISAHAASTNRA
                     DYCMAKAAMQMMTWLLADRLAEEQILVYEVCPGVIASDMTAPVKEKYDKLIADGMSPI
                     RRWGQPEDVAAAVAMLASGALGFSTGERINVDGGVHIRRL"
     gene            1422..1757
                     /locus_tag="VFV87_01405"
     CDS             1422..1757
                     /locus_tag="VFV87_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5102435.1"
                     /translation="MADKRFILNSSRSSVQGTLINVGKDSDEYVALTNTMTMLPADMA
                     EIGLAEGQMAIVRTQFGEASFKCQGGKVPAGMIFVPYGPPTCRLMGGDTDGTGMPTSK
                     GWEVEVVPV"
     gene            1785..1946
                     /locus_tag="VFV87_01410"
     CDS             1785..1946
                     /locus_tag="VFV87_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102436.1"
                     /translation="MQPISKSDITGAIESPHFSTDADGAELAAWTEIEPGRFMPRRVR
                     GSYLGRALG"
     gene            2043..2390
                     /locus_tag="VFV87_01415"
     CDS             2043..2390
                     /locus_tag="VFV87_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102437.1"
                     /translation="MTLQIIENATCTFCGCVCDDINLHHDEVRIHKAERACVLGTAWF
                     LNHTAEKKYPVALVDGKEATLDEAIEAAADLLWAADMPLVYGLSNTTCEAQKECAALA
                     DQLGGILDSHTSL"
BASE COUNT          507 a          777 c          792 g          480 t
ORIGIN      
        1 agccggggct atcgcctgca cgtcggccgc tcgatcgccg atatcgaatg ccgataccag
       61 ctcgtgccct ccttcgctca ctgcccagcg gaccaaggtc agcgactcgt catcagcgcc
      121 aagcagggcg agcttcattt ccgaattgta gcgtgcgctc gtttaacggc gagccgcagc
      181 ggcgtagggt gggctgtgcc caccatctcg cgagtggaat ggtgggcaca gcccacccta
      241 cagccggcgg atatggaccc cgccatcgac gttgatccgc tcgccggtcg aaaagccgag
      301 cgccccgctg gcgagcatcg cgacggcggc cgcgacgtcc tccggctggc cccagcggcg
      361 gatggggctc atgccgtcgg caatcagctt gtcgtatttc tccttgaccg gcgcggtcat
      421 gtcgctggcg atcacgcccg ggcagacttc atagacgagg atctgctctt cggccaggcg
      481 gtccgccaag agccaggtca tcatctgcat cgctgccttg gccatgcagt aatcggcccg
      541 gtttgtcgaa gcagcatgcg cggagatcga cgagacgttg acgatcgttc cgcggctgat
      601 cgcctgggct tcgatcagcc cgatcatctt gcgggccgcg agctgggcga ggaaaaacgg
      661 ccctttcagg ttcgtcgcga agaccgcgtc ccagctctcc tcggtcgcct ccagcaaatc
      721 cttgcggccg atcgacgtga tccccgcgtt gttcaccagc acgtccagcc ggccaaagcg
      781 atcgagcaga tccgccgcca tcccctgccg atcggctgcc tgggccacat cagcctggaa
      841 tggcagcgcc ctgccacccg cttgctcgat ctccttcacg acctccgctg cggcgttcgc
      901 gttcgaatgg taattcacgc cgaccgcata accctcgcgc gccagccgca ccgcgatcgc
      961 ccggccaatc ccgcgtgaag cgccggtgac gagagcgacg ggattggtgc tcatggtcat
     1021 ggaatttagc aacaggcggc aacccaagtc gttgctgttc ttgaaacatg ataaccctgc
     1081 gtgcgcccca attcttctgg ctgcactttg atcgagacgt ttgacgactc caactaggac
     1141 aaattcattc ccgcactttc ctgctacgcc gatctaagtc acggcaacat tgcatgtgag
     1201 ggtttcttct cttcacctta gggcggcatt cgacacgcgg gccagcaatt actccggcaa
     1261 gcttctcgct caggggtgtg gacgatctcg aaaaggagca gaatggcgaa agtttgcttt
     1321 gtcttgaaca tgatcgccgg gagcgaccat gtggattgtc gcagttgaac gccggaagta
     1381 gaattcaacg ttcgctgttc tacttggaat tgatctaacc aatggctgac aaacggttta
     1441 tcctcaattc gtcgcgctcc agcgtacagg gcacattgat caacgtgggg aaggattccg
     1501 atgaatacgt ggccctgacc aatacgatga cgatgctgcc ggcggatatg gcggagatcg
     1561 ggctggccga gggacagatg gcgatcgtgc ggacgcagtt tggcgaggcg tcgttcaagt
     1621 gccagggtgg caaggtgccg gcggggatga ttttcgtccc ctacggcccg ccgacttgcc
     1681 gcttgatggg gggcgacacc gatgggacgg gcatgccgac ctccaagggc tgggaagtgg
     1741 aagtggtgcc ggtctgaggc aaagtgaaac acgagatagc ggccatgcag ccaatatcga
     1801 aaagcgatat caccggggcg atcgaatcgc cgcatttttc gaccgatgcg gacggcgcgg
     1861 agcttgcggc ttggacagag atcgagccgg gccgcttcat gccgcggcgc gtccggggga
     1921 gctacctcgg gcgagccctg gggtgagtgt tcaacgtgcc gtcgtccggt tcggacgaca
     1981 accaatgtga agaagccaaa gcacagcaac ccgaagcgta agcgagggag tgaggaattc
     2041 ccatgaccct gcaaatcatc gagaacgcca cctgcacctt ctgcggctgc gtgtgcgacg
     2101 acatcaatct gcaccacgac gaggtccgca ttcataaggc cgagcgggcc tgcgtgctgg
     2161 gcacggcctg gttcctcaat cacacggcgg agaagaagta tccggtcgcg ctggtcgatg
     2221 gtaaggaagc gacgctcgac gaggcgatcg aagccgcggc tgacttgctc tgggcagccg
     2281 acatgccgct ggtctatggc ctgagcaaca cgacgtgcga ggcccagaag gagtgtgcgg
     2341 ccctggccga ccagctcggt ggaattctcg acagccacac atcgctttga cacgggccca
     2401 ccgaaatcgc cgcccagttg ggtggcaaag taacttgcac gttgggcgaa gtgaagcagc
     2461 gagctgactt cattgtgtac tggggcggga acccggccga gtgccatccg cgccatttca
     2521 cgaagtacac catcatgcag aagagcaagt ttctgc
//
LOCUS       DASUGB010000074         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_565339, whole genome shotgun sequence.
ACCESSION   DASUGB010000074 DASUGB010000000
VERSION     DASUGB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565339"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFV87_01420"
     CDS             <1..669
                     /locus_tag="VFV87_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102438.1"
                     /translation="THPQSYYGNGAVSCDFPGLARGIRDQELAGVRHVHFNGAGGNVT
                     AGKYNDGSPENRPVLAQRLASGMKAAWESAQKHEIKAGDVEWRIAPAALPPAAYLNEK
                     EQRSILEDEKADARQRLRGARNLAWLLRCQAGEKIDVTCLRLGPAYVLHLPGELFVEY
                     QLAAQQMRPKSPVLMAAYGDYGPGYIGLEASYGQGGYETGPVSRVAPGVEHSLMTAMR
                     ELLK"
     gene            697..2136
                     /locus_tag="VFV87_01425"
     CDS             697..2136
                     /locus_tag="VFV87_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102439.1"
                     /translation="MDPTTALQSSRRHFLAQQAMGIGSLALTWLLARDAAAAPAKPTL
                     EKPTFDLLPKAPPRQAQARAMISIFMQGGPSHIDLFDPKPELTKRHLQSFTGDIKYDN
                     AAEASAKLFGSPWKFQPHGECGMELSELLPGLAEVADDITLVRSMHTGVNNHGQSINA
                     LNSGRSTGGRPVLGSWLTYALGSEADNLPAYMVLSDPTGLPVLGVDNWTNGWLPSLYQ
                     GTVVRATEPRILNLDPPPHITPAAQERYLLFLEGLNREHLAARPGEDDLAARIASYEL
                     AAKMQTAAKEALDLSGETAETLRLYGIDQPESKDWGTRCLIARRLVERGVPFVQVCSG
                     NQTWDHHGGIEKALPLRCGQTDRPTAALVQDLKRRGLLETTIVHWGGEMGRLPVIQNE
                     KNIGRDHNTYGFSVWLAGGGFKGGHVHGATDELGHKAVESVVNHYDYHATLLHLFGLE
                     PDKLIFQRANGPGSLIDNQPARVVEELLA"
     gene            2234..2917
                     /locus_tag="VFV87_01430"
     CDS             2234..2917
                     /locus_tag="VFV87_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102440.1"
                     /translation="MESWFADAKRITSLEAEICFLLQDDFYFGPDSYPEVAERKEVER
                     LPSPNIMRHVPSMVRSAEAVGHEEELAKYYREVIRVAREHRKTFNDIRQYFWIRFWLW
                     NTDEQVHISFPWYDTYSEMNEFLTSVTNETEGQVFWDADQGWEFEMYNCNGELFARLR
                     NPDDDETHAVIRFPHEILLGQIAPLRNRTEAIIRFLSSAIGHDVWTHHVEWKEFCQTQ
                     EPKAVQRRP"
     gene            3062..3751
                     /locus_tag="VFV87_01435"
     CDS             3062..3751
                     /locus_tag="VFV87_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5102441.1"
                     /translation="MSTVRTAVALCFALSVFAPGIVRGEENWTPLFDGKSLTGWKQHG
                     GKANYRVEDEQIVGSSVPNTSNSFLCTEKEYGDFILELEFKVDPSLNSGVQIRSQVFD
                     EPREAEINGKKFKFAADRVHGYQVEIDPSDRAWSGGIYDEGRRGWLNDLKNNEAARKA
                     FKQGEWNKFRVECRGDSIKTWLNDVPAADLKDGVTPKGLIALQVHGVGGRTEPLSVRW
                     RNIRIQELDGK"
BASE COUNT          815 a         1209 c         1308 g          688 t
ORIGIN      
        1 acgcacccgc agagctacta cggcaacggg gcggtgagct gcgattttcc tggcctggcg
       61 cgcgggattc gcgaccagga gctggccggc gtgcggcacg tgcacttcaa cggcgcgggg
      121 ggcaacgtga cggccggcaa atacaacgac ggctcgccgg agaaccggcc ggtcctcgcc
      181 cagcggctgg ccagcggcat gaaagcggcc tgggagtcgg cccagaagca cgaaatcaag
      241 gccggtgacg tcgagtggcg catcgcacct gccgcgctgc cgccggccgc ctacttgaac
      301 gagaaagagc agcggtcgat cctcgaagac gagaaggccg acgccaggca gcgtctgcgg
      361 ggcgcccgca atctggcgtg gctgttgcgc tgccaggccg gcgagaaaat cgacgtcacc
      421 tgcctgcggc tggggccggc ttatgtcctg catctgccgg gcgaattgtt cgtcgagtac
      481 cagctcgccg cccagcagat gcggccgaaa tcgcctgtgc tgatggcggc gtatggtgac
      541 tacgggccgg gttatattgg cctggaggct tcgtacggcc agggcggcta cgaaacgggg
      601 ccggtctcgc gcgtcgcccc gggagttgag cactcattga tgacggcaat gcgcgagctg
      661 ctgaaatagg cggtcgcaac cagtaccatg aaacgtatgg atcccaccac cgccctgcaa
      721 tcgtcccgcc ggcatttcct cgctcaacag gcgatgggaa tcggctcgct ggcgctgacg
      781 tggctcctgg ctcgcgatgc cgccgcggcg cctgccaagc cgacgctcga aaagccgacg
      841 ttcgacctcc tgccgaaagc gccgccgcgc caggcccagg cgcgggcgat gatctcgatc
      901 ttcatgcagg ggggcccgag ccacatcgac ctcttcgacc ccaagcccga gttgacgaag
      961 cggcacctgc aatcatttac cggcgacatc aagtacgaca acgccgccga agccagcgcc
     1021 aagctgttcg gcagcccgtg gaagttccag ccgcacggcg agtgcggcat ggagctttcg
     1081 gagctattgc cggggctggc ggaagtggcc gacgacatca cgctcgtccg ctcgatgcac
     1141 acgggggtca acaatcacgg ccagtcgatc aacgccctca attccggccg ctccaccggc
     1201 gggcggccgg ttctgggatc gtggctgaca tacgccctgg ggagcgaggc cgacaatctg
     1261 ccggcttata tggtgctgag cgacccgacg gggctgcccg tgctgggcgt cgataactgg
     1321 accaacggct ggctgccctc gctgtatcaa ggcacggtgg tgcgggccac ggagccgcgg
     1381 attctgaatc tcgatccgcc gccgcacatc acgccggccg cgcaagagcg gtatctcttg
     1441 ttcctggaag ggctgaaccg cgagcacctg gcggcgcggc cgggcgagga cgacctggcg
     1501 gcccgcatcg ccagctatga actggccgcc aagatgcaga ccgccgcgaa ggaggcgctc
     1561 gacctctcgg gcgaaacggc cgagacgctg cgcctgtacg gcatcgacca gccagagagt
     1621 aaggactggg ggacgcggtg tctgatcgcg cggcggctgg tcgagcgggg cgtgccgttc
     1681 gtgcaggtct gctcgggcaa tcagacgtgg gaccatcacg gcgggatcga aaaggccctg
     1741 ccgctgcgct gcggccagac cgaccggcca accgcggctc tggtgcagga cctcaagcgc
     1801 cgcgggctct tggaaacgac catcgttcac tgggggggcg aaatgggccg cctaccggtg
     1861 atccagaatg agaagaacat cggccgcgac cacaatacgt acggcttcag cgtgtggctg
     1921 gccggcggcg ggttcaaagg cggccatgtc cacggggcga ccgacgagct ggggcacaag
     1981 gcggtggaga gcgtcgtcaa ccattacgac tatcacgcaa ccttattgca cctgttcggc
     2041 ctggagcccg acaaactgat cttccagcgc gccaacggcc ccggcagcct gatcgacaac
     2101 cagccggcac gggtggtgga ggagttgctg gcgtgattcg tggggtgcat ttcggccgcg
     2161 gcggcctcat gcaccctact tcctaaagaa ttaggttgag ccggacaaga atagttctgc
     2221 ccattaagtc atcatggaat cgtggttcgc ggatgcgaaa cgaataacgt cattggaggc
     2281 agagatttgc tttctgctcc aagacgattt ctacttcggg cctgactcat atcctgaagt
     2341 tgcggaacgt aaggaggtgg aacggcttcc ttcaccaaat atcatgcgtc acgtcccttc
     2401 gatggttcgt tccgctgagg cggtcggcca cgaagaagaa cttgcgaagt actatcgcga
     2461 ggtaattcgc gttgcaagag aacatcgcaa gacattcaac gacattcggc aatacttctg
     2521 gatacgtttt tggctatgga acacggacga gcaggttcac atttcattcc catggtacga
     2581 cacgtactct gagatgaacg aatttctgac cagcgtcacc aacgagaccg aagggcaggt
     2641 gttctgggat gcggaccaag gctgggaatt tgaaatgtac aactgcaatg gcgaattgtt
     2701 cgcacgtctt cgaaatcctg atgatgatga aacacacgcg gtcatcaggt tcccgcacga
     2761 aatattgctc ggacagattg cgccgctccg gaatcgcacg gaagcaatca ttcggtttct
     2821 ttcatcggca attggccacg acgtgtggac acatcatgtg gaatggaaag agttttgcca
     2881 aacgcaagaa cccaaagctg tgcagcgtcg cccatagtgg aacccgtgga ggggcataac
     2941 aaacggttga gcgtgagcgg cgcggggcgg cctcatgcat cctacttcct gacaatcgcg
     3001 cgggtcgcaa ggtacaatcg cccaaagttc aacccatcga catcttccga aaggccctcc
     3061 catgtccacc gttcgcaccg ccgtcgccct ttgtttcgct ctgtccgtct tcgcccccgg
     3121 catcgtccgc ggcgaggaga actggacgcc gctgtttgac ggcaaatcgc tcaccggctg
     3181 gaagcagcac ggcggcaagg cgaactaccg ggtggaggac gagcagatcg tcggcagcag
     3241 cgtgcccaac acgagcaact cgttcctctg caccgagaag gaatacggcg atttcatcct
     3301 ggagctggaa ttcaaggtcg atccgtcgct caattccggc gtccagatcc gtagccaggt
     3361 gttcgacgag ccgcgggaag ccgagatcaa cggcaagaag ttcaagttcg cggccgaccg
     3421 ggtccacggc tatcaggtgg agatcgatcc atcggatcgt gcgtggagtg gcggcattta
     3481 tgacgaaggc cgccgcggct ggctgaacga cttgaagaac aacgaagcgg cccgcaaggc
     3541 gttcaagcag ggggagtgga acaagttccg cgtcgaatgc cgcggcgatt cgatcaagac
     3601 ctggctcaac gacgtgccgg ccgccgacct gaaggacggc gtgaccccca agggtctgat
     3661 cgcgctgcaa gtccacggcg ttggcggccg taccgagccg ctttccgtcc gctggcggaa
     3721 cattcgcatt caggagctgg acgggaaata atggtgggag gaggaaccac ggaagacacg
     3781 gatgacacgg aagaagcaga aaagatagtg agcgggccgg cggtgggccg aatctggggc
     3841 ggttcggctc ctggataact gcgccatgac gaccagccac gatcgccgcc cgccgcgcgt
     3901 tcgtttttcg ctcgcccacc tgtttgctgc gacgacgctg gtcgccatcg cgttggcggc
     3961 cacgttcggg ccgcgatggg atgcggacga gcagtggatc atcggcagtt ggtttgtcgg
//
LOCUS       DASUGB010000075         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_9852,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000075 DASUGB010000000
VERSION     DASUGB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9852"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFV87_01440"
     CDS             <1..637
                     /locus_tag="VFV87_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102442.1"
                     /translation="VELQENGWSLKALHRLIVTSHTYRQSSADRADLRAVDPSNLLLG
                     HQRRLRLEAEVIRDVSLASSGLLSRKLGGPPVFPPIPDGVMGQGQVKHAWTVSQGEDR
                     YRRGIYTFVYRATPPPSLNVFDAPDGFSTCTRRVRSNTPLQALTLLNDAAFVECAMAL
                     KNVIQEEGLEAAFRRCTARQPQADELTVLRKLDALGAARVLLNLDETVTRE"
     gene            643..2094
                     /locus_tag="VFV87_01445"
     CDS             643..2094
                     /locus_tag="VFV87_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102443.1"
                     /translation="MDAQTLALQQQTRRHFFSRCSVGIGSLALASLLADRGSAAPTVK
                     LRNPLAARPPHFPAQAKNVIFLFMAGGPSQLETFEHKPKLAELNGKPIPASFVEGKRF
                     AFMDSSHRNDLLASKRTFQQYGSSGAWVSDLLPYTAGIVDELAIVTTCKTDLFNHAPA
                     KLFMNTGTGQFGRPSIGAWVTYGLGSECDDLPGFVVLQSGPRGPRGGAVLWGSGILPT
                     TNQGVPLRNQGDPILNLSTPDSVGGNDQRRVVDAVRELNLKRLVETGDEEIATRISAY
                     EMAYRMQTSAPELMDTGSESADTLDLYGIRDAGEASFARNCLLARRLVERGVRFIQLY
                     HTNWDHHGGPSENLETHLPAICKEIDQPAAALIRDLKRRGLFDSTIVIWGGEFGRTPM
                     GEVRESTGRNHHIDAFTMWFAGGGFKAGQVYGKTDELGFGAVENPVHVHDLHATILHQ
                     LGFDHERLSVKLQGLDFRLTGVNPAKVVRDLVS"
     gene            complement(2101..2769)
                     /locus_tag="VFV87_01450"
     CDS             complement(2101..2769)
                     /locus_tag="VFV87_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX5102444.1"
                     /translation="MARILITSGPTRQYLDPVRYLTNASSGRMGRALASAALELGHRP
                     VVVSGPVAIEYPSAAEIRHVVSTEELLAACQELFPTCDGAIGAAAPCDYRPIRVEAHK
                     IAKTGQPLALHLVETPDVMATLGASKRPDQWLVGFALETDDPRFRALAKLERKSCDLI
                     VLNGPGAIDAADNQVEVLDRSGVVIAQFAGSKEHVAAGILRTISARLIPGAPAAPQRR
                     ESAV"
BASE COUNT          557 a          923 c          912 g          551 t
ORIGIN      
        1 ggtcgaatta caggagaatg gctggagctt gaaggcgtta catcgactga ttgttacctc
       61 gcacacctat cggcagagtt cggctgatcg agctgatttg cgcgccgtcg atccgagtaa
      121 cttgctcctc ggccatcagc ggcgcttgcg gctggaggca gaagtgattc gagatgtctc
      181 gctcgcgtcc agcggccttt tgtcgcgcaa actcggaggt ccgccagtgt ttcctccaat
      241 cccggacggc gtcatgggtc aggggcaggt gaaacacgcg tggacggtaa gtcaggggga
      301 ggatcgctat cggcgcggca tttacacgtt cgtttatcgc gccactcctc caccgtcgct
      361 gaacgtcttt gatgcgccgg acggcttcag cacctgcacg cgccgcgtgc gcagcaacac
      421 gccgctgcaa gccctcacgc tcctcaatga cgcagcattt gtggagtgcg cgatggcttt
      481 gaagaacgtg attcaggaag aaggcttgga agcggcgttc cgccgctgca cggctcgcca
      541 gccacaggcc gatgaactca cagttctgcg caagctcgat gcgctcggcg ccgctcgcgt
      601 tctgctcaat ctcgacgaaa cggtcaccag ggagtagttg ccgtggatgc ccaaaccctc
      661 gctctccagc aacagacgcg acgccacttc ttcagccgct gctccgtggg catcggctcg
      721 ctagcactgg cttcgctgct ggccgatcgc ggttctgccg cgcccaccgt caagttgcgg
      781 aacccgctgg cagccaggcc gccgcatttt ccggcccagg ccaagaacgt gatttttctg
      841 ttcatggccg gtggtccgtc gcaactcgag acattcgagc ataagcccaa gctcgcggag
      901 ctgaacggca aaccgattcc ggccagcttc gtcgaaggca agcggtttgc gttcatggat
      961 agcagccacc ggaacgactt actggcgtcg aagcggacct ttcagcaata cggcagtagc
     1021 ggcgcttggg tcagcgattt gcttccctac accgcgggca tcgtggacga gctcgccatc
     1081 gtcaccacgt gcaagacgga tctgttcaat cacgccccgg ctaaattgtt catgaacacc
     1141 gggaccgggc agttcggccg gcccagcatt ggcgcctggg taacctacgg gctggggagc
     1201 gaatgcgatg acctgcccgg cttcgtcgtc ttgcaaagcg gcccgcgcgg cccgcgcgga
     1261 ggcgccgtcc tctggggtag cggcattctt ccaaccacga accagggcgt gccgctgcga
     1321 aaccagggcg atcccatact caatctttca acgccggact ccgttggcgg aaatgatcag
     1381 cggcgcgttg tcgatgccgt gcgggagctc aacctcaagc gactcgtgga gacgggcgac
     1441 gaagagatcg ccacgcgaat cagcgcctat gagatggcct atcgcatgca aaccagcgcc
     1501 ccggaattga tggataccgg cagcgaatcg gcggacacgc tggacctgta cgggattcgc
     1561 gacgccggcg aagcgagctt cgcgcggaat tgcttgctgg cgcgccggct ggtggagcgt
     1621 ggggtccggt tcattcagct ttatcacacg aactgggacc atcatggcgg gccgagcgaa
     1681 aacctggaaa cacacctccc ggcgatctgc aaagaaatcg accagcctgc ggcagcgcta
     1741 attcgcgacc tgaaacggag ggggctgttt gacagcacga tcgttatctg gggaggagag
     1801 ttcgggcgca ctcccatggg cgaagttcgc gaatcgaccg gccggaatca tcatatcgac
     1861 gcattcacca tgtggtttgc cggtggcggc ttcaaggctg gacaggttta cggcaagacc
     1921 gacgaactgg gcttcggcgc agtagagaac ccagttcatg tccacgacct gcacgcaacg
     1981 atcctgcatc aactagggtt cgaccacgaa cggctatccg taaagttgca gggactcgat
     2041 ttccgtctta ctggcgtcaa ccctgccaaa gtggttcgcg acctggtgtc ttaaccgatg
     2101 tcagacggcc gactctcggc gttgcggggc ggccggggct cccggtatta ggcgcgcgct
     2161 gatcgtccgg agtatgccgg ctgccacgtg ctctttgctg cctgcgaatt gggcgattac
     2221 gacgccactc cggtcgagca cctcgacctg gttgtcggcg gcgtcaatcg cgccggggcc
     2281 gttgaggaca atcaggtcgc agctcttccg ctccagtttg gccagggccc ggaaccgcgg
     2341 gtcgtcggtt tccagggcga agcccacgag ccactgatcg ggccgcttgg aagcgccgag
     2401 cgttgccatc acgtcggggg tctcgaccag gtgcagcgcc aggggctggc cggtcttggc
     2461 gatcttgtgt gcctcgacac gaattggccg atagtcgcac ggagcggcgg cgccaatcgc
     2521 gccgtcgcac gtcggaaaca attcctgaca agcggccagt aactcctccg tcgagacgac
     2581 gtgcctgatc tccgcggcgg agggatactc gatcgccacc ggaccactca ccacgaccgg
     2641 gcggtggccc aactccagcg cggcggaggc caaggcgcgg cccatccgtc cgctggaggc
     2701 attcgtcaga tacctcaccg gatcgagata ttgacgtgtc ggacccgacg taatcaagat
     2761 gcgcgccatg actccattct gcaatctgcg atcttcggtc tgcaatcgcc ctacgccgtg
     2821 gccaacgccg tcgccttctc caagaccgtg tcgagctctt cccaaatgat cgccagcgtc
     2881 acctgatcga gtcccaatcc ggaatagacc tcatcgggcg gcggaggcac attgtgaacg
     2941 ccg
//
LOCUS       DASUGB010000076         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1816542, whole genome shotgun sequence.
ACCESSION   DASUGB010000076 DASUGB010000000
VERSION     DASUGB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816542"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..404)
                     /locus_tag="VFV87_01455"
     CDS             complement(186..404)
                     /locus_tag="VFV87_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102445.1"
                     /translation="MSAETISKLVILDVLKRTPPEARDAAPAVWATVEHFLPELNDEQ
                     AALVVSLILDICPNCLAASRTCGCWQDD"
     gene            complement(520..768)
                     /locus_tag="VFV87_01460"
     CDS             complement(520..768)
                     /locus_tag="VFV87_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102446.1"
                     /translation="MAPCTCHTDRLTLLAPDPDAMQPQPRRFDQESSPLTAVERSHQT
                     AAAPAETVENLSSRIQELAGELELLCLRLRMLNRQPRA"
     gene            complement(1158..1826)
                     /locus_tag="VFV87_01465"
     CDS             complement(1158..1826)
                     /locus_tag="VFV87_01465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX5102447.1"
                     /translation="MPQEKWTAVDEYLAGRYVPADPALDAALEASRAAGLPDIQVSPC
                     QGKLLMLLAQLARAERILEVGTLGGYSTIWLARGLVPGGRLISLELNPQHAAVAQENL
                     ARARVADQVEIRIGPAAESLRQLAAENDEPFDLIFLDADKASLPQYLELSLALSRPGT
                     LLIADNVVRKGQVLDPHSPDPDVQGVRLFHERLATHPRVNGTALQTVGLKGHDGLAIA
                     LVTG"
     gene            complement(1795..2709)
                     /gene="kdsB"
                     /locus_tag="VFV87_01470"
     CDS             complement(1795..2709)
                     /gene="kdsB"
                     /locus_tag="VFV87_01470"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5102448.1"
                     /translation="MEVQALDVIVAGAHFSLGGSGSPPKRVRQSSAIVIPARLASTRL
                     PRKLLLADTGKPLIQHTYEAALQATRPGGVLVAADSEEIAAAVRRFGGRVELTSPDCA
                     SGSDRVAEIACRLPNVDIVVNVQGDEPEISAQAIDLAVELLESDPTAAMSTLAAPIRS
                     RERLLDPSCVKVVLDGRGRAIYFSRAPIPFARTWDDALLAADPPHYYQHIGLYAYRRD
                     FLLQLASLPRPPIERLENLEQLRVLYAGHPILVGMIGDPTTGIDTPDDYKAFVSRCCG
                     SSASHDCGDALSAGIRGTHAPGKMDRRR"
BASE COUNT          554 a          819 c          933 g          506 t
ORIGIN      
        1 ggatcgaggc gaaggggaca aacagcttgc tgtggggacg ctccaggaga atgcccgcgt
       61 catcgacata aaacagcgtc ccttgcaacg tcgcaggaat tgccgagttc agcgtgattt
      121 tcaccggaat gttgagccaa ccgtgaagtg ccgttgacat ggcaattcgc tcctggaccg
      181 acgacctagt cgtcttgcca gcacccgcag gtccgggagg cggccaggca gttcggacaa
      241 atatcaagga tcaagctgac gaccagcgcg gcttgctcgt cattcagttc tgggagaaag
      301 tgttcgaccg ttgcccagac ggccggcgcc gcgtcgcgag cctcaggcgg cgtgcgtttc
      361 agcacatcca aaatgaccag cttggagatt gtttccgcgc tcatttgcgc accctcaagc
      421 accgctaagg gccagtgcct gatattcggt acgccaggcg cggatcgccg gttgaaggct
      481 ggcgcgtttg taatctgcca acggcaggag cgacgccgcc taagcccgcg gctgacgatt
      541 cagcattcgc aagcgcaggc acagcaattc gagctcgcca gccaattcct gaatgcgaga
      601 ggagagattc tcgaccgttt cggctggggc agcggctgtt tggtgactcc gctcgactgc
      661 cgtgagcgga gaggactcct ggtcgaaacg cctcggctgg ggctgcatcg cgtcgggatc
      721 gggtgctagc agcgtcagcc ggtcggtatg gcaggtgcag ggggccatgc ggggctgggc
      781 atccctcgcc agccgcggcg atagagggtg gcgatcgcca cacaagggac agatccaggt
      841 ggtcgtgcgc gattgcggca cgaccacctg gtgacacgtt tgacaaaatc gcatgggaga
      901 gtgttccgca gacattctgg attcctcgtg ggcggaggcg cgagtcgtca cgcgcagtct
      961 atgaacactc agccgcagag caagaaaact gtcgcacttg taacttcaaa gattacggcc
     1021 agcttcgcgg gggttaacga actgacatct cgtctgacaa gagacgcttt gaaaatccca
     1081 agcattggtt cagcgacgtt gccgcttgcg cgctatgacc aacgtttgca acggagtctg
     1141 tgcttcagct cagccgttca accggtgacc agtgcgatgg cgaggccgtc gtggcctttg
     1201 agtccaaccg tttggagggc ggtgccattg acgcgggggt gggtagcaag gcgctcgtgg
     1261 aagaggcgga cgccttggac gtcgggatcg ggagaatggg gatcgaggac ctggcctttg
     1321 cggacgacgt tgtcggcgat cagcagcgtg ccgggacggg aaagggccag cgaaagttcg
     1381 agatactgcg gcaggctggc cttgtcggcg tcgaggaaga tcaggtcaaa gggttcgtcg
     1441 ttttcggcag ctaattgccg caggctctcg gcggcagggc cgattcgaat ttcgacctga
     1501 tcggccacgc gggcacgggc gaggttttcc tgggcgacgg cggcatgctg cgggttcaac
     1561 tcgagcgaga tcagccggcc gccgggcacc aggccacggg cgagccagat tgtgctgtag
     1621 ccgcccaagg tgccgacttc gaggatccgc tcggcccgcg cgagctgcgc gaggagcatg
     1681 agcagctttc cctggcaggg cgagacctga atatcaggca gaccggccgc ccggctggct
     1741 tccagcgccg catcaagcgc cggatcggcg gggacatagc gccccgccag gtattcatcg
     1801 acggcggtcc atttttcctg gggcatgtgt tcctctgata cctgccgaca gcgcgtcgcc
     1861 acagtcgtgg ctcgcagaag atccgcaaca ccggctgaca aacgctttgt agtcatctgg
     1921 cgtatcgata cccgtggttg gatcgccgat catgccgacg agaatcggat gaccagcgta
     1981 caggacccgg agctgttcca aattctccag gcgctcgatg ggcggccgtg ggagcgaagc
     2041 gagttgcagg aggaagtcgc ggcgataggc atagagaccg atgtgctggt agtagtgcgg
     2101 tgggtcagcg gcgagcagcg cgtcgtccca cgtgcgggca aacggaatcg gggcgcggct
     2161 gaagtaaatc gcgcggccgc ggccgtcaag aacgactttg acgcaggacg ggtcgagcag
     2221 cctttcgcgc gagcgaatag gtgcggctaa cgtgctcatt gccgcggtcg ggtcgctttc
     2281 gagcagctcg accgcgaggt cgatcgcttg ggcagagatc tctggttcgt ctccctggac
     2341 gttgacaacg atgtccacat tgggcaacct gcaggcgatt tccgcgacgc gatcggagcc
     2401 gctggcgcaa tccgggctgg tcaattcgac gcgcccgcca aatcgccgta ccgccgccgc
     2461 aatttcctcg ctgtcagccg ccaccagcac gccgccaggc cgcgtcgcct gcaaagccgc
     2521 ctcatacgtg tgctggatca gcggcttgcc ggtatccgcc aaaagcagtt ttcgcggcaa
     2581 gcgcgttgat gccagccgag ctggaatcac aatcgcgctg ctctggcgga cgcgtttagg
     2641 cggtgaacca gagcctccga gcgagaagtg cgcgcccgca acgatgacat ccaacgcttg
     2701 gacttccatg tctttgcctc ttgggaaagg aaacatggta atccgtccgc cgaagcgccg
     2761 cctacagcga aataagccct cagcaagtca agaacagaag acgcccggcc aa
//
LOCUS       DASUGB010000077         6917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_10315,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000077 DASUGB010000000
VERSION     DASUGB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10315"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFV87_01475"
     CDS             <1..610
                     /locus_tag="VFV87_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX5102449.1"
                     /translation="GVQTRDNVHVGFKWIAQEIDAGGSDKFVFGCEESFGFLIGQYVR
                     DKDGAAACMLMAELAAACKAAGKTLFDRLESLYWQHGYHAERQFNLFMEGSTGMARMK
                     SLMQQFRSTPPKSLGGIAVKGVRDYLGLPDAPTGDMVILDLATHAHYVAVRPSGTEPK
                     LKFYLFTHVPAEQLHLLDEARVEMDQRLTAIEAELRALAVAI"
     gene            781..1236
                     /locus_tag="VFV87_01480"
     CDS             781..1236
                     /locus_tag="VFV87_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102450.1"
                     /translation="MPSGPVVECVCGRRFQAIAQVMGQTVSCPACQQPLTIPSTYQSI
                     FDDLPPGTIQPPRPARPPKPAMPFASQPPARRWKPHPVASEFWRGAKILCGCLLLLGG
                     GLQLLMALISVMAAAQQNQPVAVGLKLFGSAAISLIVGWRMAFPAESNA"
     gene            1246..2721
                     /locus_tag="VFV87_01485"
     CDS             1246..2721
                     /locus_tag="VFV87_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5102451.1"
                     /translation="MAGFDELGHDDEGEELEWAHSAAASEEPAALELSIDEAELDAAL
                     AAVAADESGEHDFAFRRAAAKVREFPRKPGIYLMKDAAGRVIYVGKAKNLRARAGSYF
                     LKGALAERRTADWVGEIADVDFVECDSDVDALLMEARLIKDVQPKYNKELKDDKTFPY
                     LMITTREDFPRVEVTRKPPVRGVRLFGPFANAGALRGAVQVLQRIFKFRTCSLDIEDG
                     DDRWRWFRPCLLASIKQCTAPCNLRISKEEYRKDIDRLIRFLEGGKVRLLAEMKGEME
                     QLAKELKYEAAARLRDEIKLLEKLDRRGDLDTHAQPEVFYIDPKKGLAGLKKVLKLTE
                     QPRTIEGVDIAHLGGGQTVASVVQFLDGLPFKPGYRRYKVAGVQGVDDFRSIHEVIAR
                     RYRRLSEEDEVFPDILLIDGGKGQLNAALAAFRDQEITPPTLLSLAKQDEEIFLPGES
                     EPLKLSRHSYALRLLQYVRDEAHRFAQHYHHILRRKSQLGE"
     gene            2791..3663
                     /locus_tag="VFV87_01490"
     CDS             2791..3663
                     /locus_tag="VFV87_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102452.1"
                     /translation="MALTFRIHGIALLLLVGFTAAAQEPRTKEPPAERPPDPREREKL
                     EFFRDRIGGVKLMVADRDEPCQYMAEPVAKFDNPISHIEDGFMFLWTDRGRPVAAVKS
                     YHNLPNGTWGRTFVSLATQRIVMQQDGKSLWTPEQKGVTFAELPDAPAPAAEARQRLV
                     QMRAIARRFSVIDNWGIKDPTDWQLRLLTTPVYRYESQDNDVVDGALFGYVLTSPEAL
                     LLLEARKTDKGLAWHYAVARFTRFGVRFSLDGQQIAEFERLDAWPSTGTYFHYPQPWE
                     EYPFESSQETSPSR"
     gene            3694..4644
                     /locus_tag="VFV87_01495"
     CDS             3694..4644
                     /locus_tag="VFV87_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102453.1"
                     /translation="MRSKAFASPLPALLLALLPAAVSGQETERLAVYVLDIEKAEVQQ
                     VAAEPLEGLAYCGSPEWSYDGKRIVLDASPGKQFNKTHVLAADCPITNQTKFVDLGPG
                     NCPTWSPDAKQIAFLLNSGAVAGATPGIWIMNADGQGRRHLGGGGVPQWSPDGKTILT
                     SSFSNPTELAILDVATGDEHAITLKDHVFYSVPNWANDSQTLIAVIRAEGPQMIALVD
                     VADPAAAKVKEVLWTRGQGISAEPMYPTYSAATKRVAFAGRTPNGSSLYLLESRTSFT
                     PKPLEPDRYDAKIASLAISPEGRHLLFCSDREMDPPNNAD"
     gene            complement(4677..6518)
                     /locus_tag="VFV87_01500"
     CDS             complement(4677..6518)
                     /locus_tag="VFV87_01500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5102454.1"
                     /translation="MAVASTDEFVSLVKRSGLITDDQLQSFLARSVLPPEDPLAMAGA
                     LSDAGLVTPWQREKLLEGRFKGFFLGKYKVLDQLGAGAMGNVFLAEHRVMRHRVAIKT
                     LAKRLVGNDTYIRRFEQEARAAAAINHPRVVRAFDFDSTGDVYFLVMEYVEGEDLAKI
                     VLRDGVLPIPVAAECIRQTAEGLAAAHEGGLIHRDIKPSNLLVDKQGNIRILDLGLAR
                     LIDDDAPSLTLVTDSKMIGTVDYLAPEQARNSHHIDHRADLYSLGCTFYFLLCGHPPF
                     DEGTLTQRVIEHQTKKPVDIRQRRRDCPAELAFICHKLLAKTPGERFQTAQEVVEALG
                     EWLQCYKGIVGGSVDPAKIAPTSLRRAASESRAGSAGGSESELQLVPSGDTLDGIRAA
                     ASTAGSGGGLDDGDLALVDDDALVGPRPGAAPSPSGPGRTAARKPGSSVSGASKGSGS
                     GGSGSNAGASRPDSAAASGRDSALELPSPAPLPSQDSLVQLLGEQLPDALVSAKPKSN
                     PLGLADDFQLSPLDAAPEERTKTSAAAEAYLRAAAASQPKPASWRERIQALVSEEGPG
                     GIAYSLWFLIFSGMVLGLIVCVVGYAYHASTKELDVPKTRSFESRRE"
     gene            6735..>6917
                     /locus_tag="VFV87_01505"
     CDS             6735..>6917
                     /locus_tag="VFV87_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102455.1"
                     /translation="METTLHRQLKALYAGPEARTEERLEGYRIDAVRSIPGGGLRGRQ
                     LVEIQHGSLAAIRDKIA"
BASE COUNT         1301 a         2232 c         2157 g         1227 t
ORIGIN      
        1 cggcgttcaa acgcgcgaca atgtccacgt cggcttcaag tggatcgccc aggagatcga
       61 tgcaggcggc tcggacaagt tcgtcttcgg ctgcgaggaa tcattcggct ttctgatcgg
      121 gcaatacgtg cgcgacaagg acggcgccgc cgcctgcatg ctcatggccg aactggccgc
      181 tgcctgcaaa gccgccggca agacgttgtt cgaccggctc gagtcgctct actggcagca
      241 cggctaccac gccgagcggc agttcaattt gttcatggag ggctcaaccg gcatggcgcg
      301 catgaagtca ctcatgcagc aatttcgctc gacgccgccc aagtcgctgg gtggcatcgc
      361 agtcaagggc gtccgcgact atctcggtct gcctgacgct ccaacggggg acatggtgat
      421 tctcgatctc gccacgcacg cccactacgt cgctgtccgc cccagcggaa ccgagccgaa
      481 gctcaaattc tatctcttca cgcacgtccc ggccgagcag ctccatttgc tcgatgaagc
      541 acgggtggag atggaccagc ggctcaccgc catcgaagcc gaactccgtg ccttggcggt
      601 cgcaatctga tcgcaatctg agaattggtg cccgcttccc ccttcgctta taatcccgac
      661 tgtcattctg caagtgtggc ggaaatgatc gtcacctgtc gttgcgggca gtcgtatcaa
      721 gccgccgatg cgctggctgg gcagtgtatt tcttgtccgg catgtcagca gacattgcac
      781 atgccgtccg ggccggtcgt ggagtgtgta tgcggccggc ggtttcaggc gattgcgcaa
      841 gtcatgggcc agacggtctc ctgccccgct tgtcagcagc cgctcacgat tcccagcacg
      901 taccagtcga tcttcgacga tttaccaccc gggacgatcc agcctccgcg gcccgcacgg
      961 ccgcccaagc cggcaatgcc attcgcctcg cagccgcccg cgcggcgctg gaagccgcat
     1021 cccgtggcat cggaattctg gcgaggggcc aagatcctct gcgggtgcct gttgcttctc
     1081 gggggcggcc tgcaactgct gatggcgctg ataagcgtga tggcagccgc tcagcagaat
     1141 caacccgtgg cggttgggct caagctattt ggatccgcgg cgatctcgtt gatcgtcggc
     1201 tggcgaatgg cattcccggc ggaatccaat gcgtaaaatg ggcgcatggc cggttttgac
     1261 gaactcgggc acgacgacga aggcgaagag ctggaatggg cacattccgc agcagccagc
     1321 gaagagccgg ccgccctgga gctttcgatc gacgaggccg agctcgatgc ggctttggcg
     1381 gcggtggcgg cggatgagtc gggcgagcac gatttcgcct ttcgccgggc ggcggccaag
     1441 gtgcgcgagt tcccgcggaa gccgggcatc tatttgatga aggacgcggc cggccgagtc
     1501 atctatgtcg gcaaggcaaa gaacctgcgg gcgcgggcgg gcagctactt cctcaagggc
     1561 gcgctggccg agcggcgcac ggctgattgg gtcggcgaga tcgctgacgt cgattttgtg
     1621 gagtgcgaca gcgacgtcga tgccctgctg atggaggccc ggctgatcaa ggacgtgcag
     1681 ccgaaataca acaaggagtt gaaggacgac aagacattcc cgtacctgat gatcacgacc
     1741 cgcgaggatt ttccacgggt ggaagtcacg cgcaagccgc cggtccgcgg cgtgcggctc
     1801 tttggtccat tcgcgaacgc tggcgcgctc cgcggcgcgg tacaggtgct ccagcggatc
     1861 ttcaagttcc gcacgtgcag tctggatatc gaggacggcg acgatcgctg gcggtggttc
     1921 cggccgtgcc tcttggcgag catcaagcaa tgcaccgcgc cgtgcaacct gcggatcagc
     1981 aaggaggaat accgcaagga tatcgaccgg ctgattcgct ttctggaagg gggcaaagtg
     2041 cggctgctgg cggaaatgaa aggcgagatg gagcagctcg ccaaggagct caagtacgaa
     2101 gcggcggcgc gcctgcgtga cgaaatcaag ctgctggaaa agctcgaccg ccgcggcgac
     2161 ctcgacacgc atgcccagcc ggaagtgttc tacatcgatc ccaagaaggg cctcgccgga
     2221 ctgaagaagg tgctcaagct gaccgagcag ccgcggacaa tcgaaggggt cgatatcgcc
     2281 cacctaggcg gcggtcagac ggtggccagc gtagtgcaat tccttgatgg cctgccattc
     2341 aagccgggct atcggcgcta caaggttgcc ggcgtacagg gcgtggacga cttccgcagc
     2401 atccacgaag tcatcgctcg gcgctatcgc cggctctccg aagaggatga agtctttccc
     2461 gatatcctgc tgatcgacgg tggcaagggg cagctcaatg cggcactcgc cgcgttccgc
     2521 gaccaggaga tcacgccccc cacgctcctc tcgctcgcga agcaagacga agaaatcttt
     2581 ctccccggcg aaagcgagcc cctcaagctc agccgccatt cctacgcgct gcgcctgctg
     2641 caatacgtcc gcgacgaagc ccaccgcttc gcacagcact accaccacat cctgcggcga
     2701 aaatcgcagc tgggtgagta gcttgacatt cagacaattt gtcttaaaat cggcgagtgg
     2761 ttcgggcagt tgatccgttg ggaggcaagc atggccctca cctttcgtat ccacggcatc
     2821 gccttgcttc tgctggtggg ctttacagct gcggcgcagg agccaagaac caaggaacct
     2881 cccgccgagc gcccgcccga cccacgggag cgagaaaagc tcgaattctt tcgcgaccgc
     2941 attggcggtg tgaagctgat ggtggccgat cgggatgaac cgtgccaata catggccgag
     3001 ccagtggcca agttcgacaa cccgatcagc cacatcgaag acggtttcat gtttctctgg
     3061 accgatcgcg gccggccggt ggccgcggtc aagagctatc acaacctgcc gaacggaacc
     3121 tggggccgca cgttcgtctc gctggccacg cagcggatcg tgatgcagca ggatggaaaa
     3181 tcgctgtgga cgcccgagca gaagggcgtg accttcgccg agttgcccga cgcgcccgct
     3241 cccgctgcgg aggctcgcca gcgactggtg cagatgcggg ccatcgcccg ccgcttcagc
     3301 gtgatcgaca actggggcat caaagacccg accgattggc aactgcggct cttgacgact
     3361 cccgtgtatc gctacgagtc ccaagacaat gacgtcgtcg atggtgcgct gtttggctac
     3421 gtgctcacat cgcccgaagc gctcctgctg ctcgaagctc gcaagaccga caagggcctg
     3481 gcctggcact atgccgtcgc gcggttcacg cgattcggcg tgcgcttctc gctcgatggc
     3541 cagcaaatcg ccgaattcga acgactcgac gcctggccat ccacgggcac gtacttccat
     3601 tatccgcagc cgtgggaaga gtatccgttc gaatccagcc aggaaacgtc gccgtcgcgc
     3661 taatccccgt ccgcgaatgg tggagatacg accatgcgat ccaaggcatt tgcttctccg
     3721 ttgcctgcgc ttcttctggc cctgctcccg gctgctgtca gcggccaaga aaccgagcgt
     3781 cttgccgtct acgtcttgga catcgagaag gcggaagttc agcaagtcgc cgccgaaccg
     3841 ttggaagggc tcgcctattg cggctctccc gagtggtcgt acgacggcaa gcggatcgtg
     3901 ctcgatgcgt ctccgggcaa gcagttcaac aagacacatg tgctggctgc cgattgcccc
     3961 atcacgaatc agacaaaatt cgtcgatctc gggccgggca attgccccac ctggtctccc
     4021 gacgccaagc agatcgcctt tctgctcaat tccggggcag tcgcgggggc gacgccagga
     4081 atctggatca tgaacgcgga tggccaaggg cgtcggcatc tgggcggcgg cggcgtaccg
     4141 caatggtcgc ccgacggaaa gacaatcctc accagctcgt tcagcaaccc cacggagttg
     4201 gcgatcctcg atgtcgccac aggcgacgag cacgctatca ccttgaagga ccatgtgttc
     4261 tactccgtcc cgaactgggc caacgattcg cagaccctga ttgccgtgat tcgcgccgaa
     4321 gggccgcaga tgatcgcgct ggtcgatgtt gccgatccgg ctgccgcgaa ggtcaaggag
     4381 gtcctgtgga cgcgcggcca aggaatcagc gccgagccga tgtatccaac ctattcggcc
     4441 gccaccaagc gcgtcgcctt tgcgggccgc acgcccaacg ggtccagcct ctacctgctt
     4501 gagtcgcgca cgagcttcac tcccaagccg ctggagccgg atcgctacga cgccaaaatc
     4561 gccagcctgg cgatctctcc cgaaggccgg cacttgctgt tctgtagcga tcgcgaaatg
     4621 gacccaccga acaacgccga ttgaatctgg tgcgcggtcg cgccgccggt ctacaactac
     4681 tcgcgtcggg actcgaacga ccgggtcttc ggcacgtcca gctctttggt gctcgcgtgg
     4741 taggcgtagc ctacgacgca cacgatcagc cccagtacca tcccggaaaa gatcaaaaac
     4801 cacagcgagt acgcgatgcc gccggggcct tcttcggaaa ccagggcctg gatccgctcg
     4861 cgccaactcg ccggcttggg ctgcgatgcg gcggctgcac gcaggtacgc ttcggcggca
     4921 gctgaggttt tcgtgcgctc ctccggcgcg gcatcgagcg ggctgagctg aaaatcgtcg
     4981 gccagaccca gcgggttgct ctttggcttc gcgctgacaa gggcgtcagg caattgctcg
     5041 cccagcaatt gcaccagcga atcctggctc ggcaaaggag cgggcgaagg cagctcaagg
     5101 gcgctatcac ggccactcgc tgccgcgctg tcgggcctgg acgcgccggc gtttgaacca
     5161 gagccgccgg agcccgaccc cttcgatgca cccgagacag agcttcctgg cttccgcgcg
     5221 gccgtccgtc cagggccgct gggcgaaggt gccgcgcccg gccgcggccc gaccagcgcg
     5281 tcgtcgtcta ccaaagccag atcgccatcg tccaatccgc cgccgctgcc ggccgtcgaa
     5341 gccgcagccc gaatgccatc gagcgtgtcg ccgctgggta cgagttgcag ttcgctttcc
     5401 gatccgccag ccgatccggc ccgcgattcc gacgcggccc tgcggagcga cgtaggcgcg
     5461 atcttcgccg gatcgaccga tccgccgacg atccccttgt agcactgcag ccattcgccg
     5521 agcgcctcca caacctcctg cgcggtctgg aaccgctcgc ccggcgtttt cgccaagagc
     5581 ttatggcaga tgaacgccaa ttctgccggg caatcccttc gccgctggcg aatatcgacc
     5641 ggcttcttcg tctgatgctc gatgacccgc tgcgtcagcg tcccttcgtc aaatggagga
     5701 tggccgcaca agaggaagta gaacgtgcag cccaggctgt agagatcggc ccggtggtca
     5761 atgtggtggc tgttgcgggc ctgttcgggg gcgaggtaat caaccgtgcc gatcatcttc
     5821 gaatcggtca ccagcgtcag cgagggtgca tcatcatcga tcagccgtgc cagccccaga
     5881 tcaagaatcc ggatgttgcc ctgcttatcg acgagcaaat tgctcggttt gatgtcgcgg
     5941 tggatgaggc cgccttcgtg cgccgcagcg agcccctcgg cggtctggcg aatgcattcc
     6001 gccgcgaccg gaatcggcag cacgccgtcg cggaggacga tcttggccag gtcctctccc
     6061 tcgacgtact ccatcaccag gaaatagacg tcgccggtcg aatcgaaatc gaacgcccgc
     6121 acaacgcgcg gatggttgat cgccgccgcg gcccgcgcct cctgctcgaa tcggcggatg
     6181 tacgtatcgt ttcccaccag ccgcttggcc agcgttttga tcgccacgcg gtgccgcatg
     6241 acgcggtgct cggccaggaa gacgttgccc attgcccccg cgccgagctg atcgagcacc
     6301 ttgtacttgc cgaggaagaa gcccttgaat cgcccctcca gcagcttctc gcgctgccag
     6361 ggagtgacca gcccggcatc cgacaaggcg cccgccatcg ccagcggatc ttccggcggg
     6421 agcacgctgc gcgcgaggaa cgactgcagc tgatcgtcgg tgatcagccc gctgcgcttc
     6481 accagggaga cgaattcatc cgtgctcgcg acggccacgg agcaccactc caatttgggg
     6541 gccgatcagg cggggcgcaa cgatcgccgc agtcgtgggg agcagggaat cggccaaagt
     6601 tgcagagtcg atccaaggtc gccgcatttc gtgaagattc ggctaggaag cggtgaagat
     6661 tccggttgcg agtgctcgtc agttcgaccg atttggcatt ggctgaacgt ggccgcagac
     6721 gtatgaacca ccacatggaa acgaccttgc accgccagct caaagccctc tatgccggcc
     6781 ccgaagcccg gaccgaagag cggctggagg gctatcgcat cgatgccgtc cgcagcattc
     6841 ccggcggtgg gctgcgcggg cgacaactgg tcgaaatcca gcatggcagc ctggcagcga
     6901 tccgcgacaa aatcgcc
//
LOCUS       DASUGB010000078         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1399350, whole genome shotgun sequence.
ACCESSION   DASUGB010000078 DASUGB010000000
VERSION     DASUGB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399350"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFV87_01510"
     CDS             <1..851
                     /locus_tag="VFV87_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5102456.1"
                     /translation="ALVLALNEPSIPNDLRIAIGATLTRSVSEGVPEETARLWIENFR
                     EVMKNLREVMKRAPSRLQSTMAETLAGDADGANALLVLVETGHASPRLLLAPNVSAKF
                     ASLKDESLDKRAAAITAKLPPFSETLDKLILERRVTFAKAAPNLEKGQAVFTKHCAGC
                     HQIEGKGSVVGPQLDGIGGRGLERIIEDILDPNRNVDVAFRTTTIRKTDGQIVSGLVR
                     REEGAQLVLADKEGKEFTLAKDDIDEQQKTSLSLMPANVPEIVPAEEFHDLIGWLLSK
                     RAAASK"
     gene            956..1684
                     /locus_tag="VFV87_01515"
     CDS             956..1684
                     /locus_tag="VFV87_01515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5102457.1"
                     /translation="MSLALADRPSPAEAKPRGLSVPLVVGPLRGCWWQLASGGKVARL
                     LLGTYEREQTALFQRHIRSGQQVLDIGAAAGYYTLLAARLVGPTGSVIAFEPNPENLP
                     YLRTHVRQNGLAHVRVLNLAVSDQDGTARFGGGTGTGTARLQADGAYEVAVRRLDDLA
                     AEHDLAPQHLKIDVEGAELAVLRGGERLIREHRPTIFLSTHDGIEPGVHRACCELLAS
                     WGYSLEPIVGTSVEAASEVLCLPA"
     gene            complement(1690..2763)
                     /locus_tag="VFV87_01520"
     CDS             complement(1690..2763)
                     /locus_tag="VFV87_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX5102458.1"
                     /translation="MRTTLRLAIACVLAAGVGSTVTAADRLIRLDIPGRTGFEGTPLA
                     WSSQQVFFLARDGQLVECDPRQATNFSEVPGGFKSCSQGELRGLLMAEFSGYEVSGVG
                     HYLVVHPAGRRDEWAPRFEELYRSFVVYFSARGWRLEEPQFPLVAVVFPRQIDFARHA
                     AREGLVASSGVLGYYSTDTNRILMYDSGASGNDWTATADTIIHEAAHQTAFNTGVHSR
                     FGTAPRWVVEGLGTMFEARGVWNSRNFPHVGDRINRGRLQAWRQYSRSRRRADAIAQL
                     VASDRPFQADADGAYSEAWALSFYLSETQPQKYSAYLARTAATRPFSTYRSQERMKDF
                     TDIFGSDLAMLDAQLQRYLAGLK"
     gene            complement(2855..>3796)
                     /locus_tag="VFV87_01525"
     CDS             complement(2855..>3796)
                     /locus_tag="VFV87_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102459.1"
                     /translation="GMYTQRLLNELSAARVTLLSPEAKKAYDDALARHLAARIVPASA
                     TQHTSQPAPAVLTFGLGQTPVVPAPPVLPPPPPASAAPRISLDDETDEEPPPEPAKPS
                     PWWRPLAVLIAASILALAGVVGWGISKPYFDHLRADRTSAELPSDADQSPEPEPPPKP
                     APKPIVLLQEGSGEVVFTPATATRSGAVELQIALTEEVLANWTGPDDAAEWRFKLVKP
                     GFFDVELSYATSAAAGEGAVEVTIGEREANIPLQPTGGIDQFHSESRPLIVPASGEHT
                     LALRPKLQLEGHSIIVRGVRLVPALTNTLPAGEATPP"
BASE COUNT          665 a         1224 c         1315 g          592 t
ORIGIN      
        1 cagcgctcgt cctggcgctc aacgagccgt cgattcccaa cgacctgcga atcgcgatcg
       61 gcgccacact aacccgaagc gtaagcgagg gagtcccgga ggaaacggcc agactttgga
      121 tcgagaactt ccgcgaagtg atgaagaacc tccgcgaagt gatgaaacgc gccccctcac
      181 gcctgcaatc gacgatggct gaaacgctcg ccggcgatgc cgacggcgcc aatgccctgc
      241 tggtgctcgt cgaaacgggc cacgcctcgc cgcggctatt gctcgctcca aacgtcagtg
      301 cgaaattcgc atcgctgaag gacgaatcgc tcgacaagcg cgccgcggca atcaccgcca
      361 agctgccgcc gtttagcgaa acgctcgaca agctgattct cgaacgccgg gtgaccttcg
      421 cgaaggccgc gcccaacctc gaaaaaggcc aggccgtctt caccaagcac tgcgccggct
      481 gccatcagat cgaaggcaag ggctcggtcg tcggcccgca actcgacggc atcggcggca
      541 ggggtttgga gcggatcatc gaggacattt tggatccgaa tcgcaatgtc gatgtggctt
      601 tccgcaccac gacgatccgc aaaaccgacg ggcagatcgt cagcggcctg gtgcggcgcg
      661 aagaaggcgc gcagctcgtc ctggccgaca aagaaggcaa ggagttcacg ctcgccaagg
      721 acgacatcga cgagcagcag aagaccagcc tctcgctcat gcccgccaac gtgcccgaga
      781 tcgtccccgc cgaggaattt cacgatctga tcggctggct gctgagcaaa cgggcggcag
      841 cttcgaagtg aggtggagcg ggcacgactg ctagcaggac gtgctggaat tgcccttgct
      901 cgcaaaggat cggacggcat acaagggccg gatcaccaac ctcggggatc atcccatgtc
      961 gctcgctctt gccgatcgtc cgtcgcctgc cgaagccaaa cctcgcggac tgtctgtgcc
     1021 gctggtggtc ggcccgctgc ggggctgctg gtggcagttg gccagcgggg gaaaggtcgc
     1081 ccggcttctc ctgggcacgt acgagcgcga gcagacggct ctgtttcaga gacacattcg
     1141 cagcgggcag caggtgctcg atatcggtgc ggcagccggt tactacaccc tgctggccgc
     1201 gcggctcgtc ggcccgaccg gcagcgtcat cgcctttgag ccgaatccgg agaacctgcc
     1261 ctacctgcgg acgcacgtgc ggcagaacgg cctcgcccat gtccgcgtgc tgaacctcgc
     1321 cgtgtccgat caagacggca ccgcccgctt cggcggcggc acggggaccg gcaccgcccg
     1381 cctgcaagcc gacggcgcat acgaagtcgc cgtccgccgg ctcgatgatc tggccgccga
     1441 gcacgatctg gccccgcaac acctgaagat cgacgtcgaa ggggcggaat tggccgtcct
     1501 ccgcggcggc gagcgattga ttcgcgaaca tcggccgaca atcttcctct cgacgcacga
     1561 cggcatcgag cccggcgtgc accgcgcgtg ctgcgagctc ttggcgagtt ggggctattc
     1621 gctagagccg atcgtcggta cgagcgtcga agcggcgagc gaagtcctgt gcctgccggc
     1681 ctgagcggct tacttcagcc cggccaggta ccgctgcaac tgcgcgtcga gcatcgccag
     1741 gtcgctgccg aagatgtcgg taaagtcctt catccgctct tggctgcggt aggtgctgaa
     1801 ggggcgggtc gcggcggtgc gggcgaggta agccgagtac ttttgcggct gcgtttcgct
     1861 gagatagaac gagagggccc aggcttcgct gtacgcgccg tcggcgtcgg cctggaaggg
     1921 ccggtcggac gcgacgagct gggcgatggc gtcggcccgc cgccgcgagc gggaatattg
     1981 acgccacgcc tgcagccggc cgcggttgat gcggtcgccg acgtgcggga agttccgcga
     2041 gttccacacg ccgcgggcct cgaacatcgt gccgagcccc tcgacgaccc accgcggggc
     2101 ggtgccgaag cggctgtgga cgccggtgtt gaaggccgtc tggtgggccg cttcgtggat
     2161 gatcgtgtcg gccgtcgcgg tccagtcgtt gccgctggcg ccggagtcgt acatcaaaat
     2221 gcggttggtg tcggtcgaat agtagccgag gacgccgctc gatgcgacca gcccctcgcg
     2281 ggccgcgtgg cgggcgaaat cgatctgccg cgggaagacg accgccacca gcgggaattg
     2341 gggctcctcg agccgccagc cgcgggccga aaaatagacg acgaacgagc ggtacagctc
     2401 ttcgaaccgc ggggcccact cgtcgcggcg gcctgccggg tggacgacga ggtaatggcc
     2461 gacgccggag acttcgtagc cggagaattc ggccatcaag aggccgcgca gctcgccctg
     2521 cgagcagctc ttgaacccgc cggggacctc gctgaagttc gtggcctgcc gcggatcgca
     2581 ctcgacgagt tggccgtcgc gggccagaaa gaagacctgc tggctcgacc aggcgagcgg
     2641 cgtgccttcg aagccggtgc ggccgggaat atcgaggcgg atcaaccggt cggcagcggt
     2701 tacggtgctg ccaacacctg cggcgaggac acaagcaatg gccagtcgca acgtggtccg
     2761 cattggcttc ctccgccagc accaaagccg cgaccagtgg tcgcggttcc cacggcttcg
     2821 gtgggcaaag gaaagcctac gttgcgaccg gcgatcaggg cggagttgcc tcgccggcag
     2881 gcagagtatt cgtcagggcc ggcacaagtc gcactccgcg gacgatgatc gagtgacctt
     2941 cgagctggag tttggggcgc agggcgagcg tgtgctcgcc gctggcggga acgatcagcg
     3001 gcctggattc ggagtggaac tgatcgatgc cgcccgtcgg ctgcagcggg atgttcgcct
     3061 cccgctcacc gatggtcact tcgaccgcgc cctcaccggc cgcggcggaa gtcgcgtagg
     3121 aaagctccac gtcgaagaag cccggcttga cgagcttgaa gcgccactcg gctgcgtcgt
     3181 ccgggccggt ccaattcgcg agcacctcct cggtgagggc gatctgcaac tcgacggcgc
     3241 cgctgcgcgt cgccgtagcc ggcgtgaaga cgacttcgcc gcttccttct tgcaacagga
     3301 cgatgggctt cggcgctggt ttgggcggcg gttcgggctc tggggactga tcggcgtcgg
     3361 aaggcagctc agcactcgtg cgatcggcgc ggagatggtc gaagtagggc ttgctaatgc
     3421 cccagcccac gacgcccgct agcgccagaa ttgacgccgc gatcagcacc gccagcggcc
     3481 gccaccaggg cgaaggttta gccggctccg gtggcggctc ttcatcggtt tcgtcgtcca
     3541 atgaaatgcg cggggcagca gacgctggcg gaggcggcgg caggacggga ggcgccggga
     3601 cgacgggcgt ctggccgagg ccgaacgtca ggaccgccgg agcgggttgc gaagtgtgct
     3661 gcgtcgccga agcagggacg attcgcgcgg ccagatgccg ggcgagggcg tcgtcgtacg
     3721 ccttcttcgc ctccggcgat agcagcgtca cgcgggccgc ggaaagctcg ttgagcagcc
     3781 gctgtgtgta catccc
//
LOCUS       DASUGB010000079         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_288006, whole genome shotgun sequence.
ACCESSION   DASUGB010000079 DASUGB010000000
VERSION     DASUGB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288006"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFV87_01530"
     CDS             complement(<1..483)
                     /locus_tag="VFV87_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX5102460.1"
                     /translation="MVRARFLSLVMIIPAAAAILGCGAEGVHGPAGDAASPAVQRELR
                     EIDASRYAAPASEAPMNLATTPAPTASAQPAAETAPAEGVLLGGKGLPMTPADDAVEQ
                     ARIDQGRGPGEGGDKFAYIDENPFLAVKDTPLSTFSIDVDTASYAKTRAYLLEHHSLP
                     P"
     gene            656..2290
                     /locus_tag="VFV87_01535"
     CDS             656..2290
                     /locus_tag="VFV87_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5102461.1"
                     /translation="MAIVQRLARTPLATWLAAAVALAAIWAAAPDAARPAMMNSAILA
                     GAATAIALPLGTLLAVLITRFDLPGRRLAAACLGVLLFLPLYVQLAGWDAAVGKIGWL
                     TLAYGHMDEPFLAGMRGAVFVHAMAAVPWVALVVGLGLSQVDPAQEEAALLILSPIEV
                     LFRVTLRQAVPFILAAAVLTVVATTSEMTVTNIFLINPMEQTFTEQFYMTFALQADAG
                     EAARSVWPAVAGLGVLITLSLWMAIHAARRTHWFSAQRPVTFTAGTLRPFAAALMWLL
                     IAVLLAIPIGSLISKAGFVVVHEGSKRVQSWSLAKCVEQVAQAPAQFSFEFRWTIITA
                     LGAATCALAAAVALAWPARRGGWRAMPAIAAVVLGLTIPGPLVGVSLIWVFNRSLWPN
                     VFDPLVYLYDRTPVVVMFAQAIHALPITLLVCWYSFRTLSDDVLAAAALDGAPPRQVL
                     WRIALPLRWQALAGAWLAAFAIAAGDVAWAQLVRPAGMDLIQRRVLGLVHSGVDEQVA
                     AISLINVIAYAVLTGLILWLVGPRRNWRRVAAAGDL"
     gene            2298..3434
                     /locus_tag="VFV87_01540"
     CDS             2298..3434
                     /locus_tag="VFV87_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX5102462.1"
                     /translation="MSRSIAVLGLLLAAAAPALGLEYVTIQQNGRQTELAGKLAVEAE
                     DGGVMLLTPDGVLWPLPKEEIVSRRSDEKPFVPLSAEELSAKLTAELPAGFKIHQTKN
                     YLICYNTSPAYAQWVGGLFERLNGAFYNYWTRRGVEMRPPQFPLVALVFDSKSSYAQH
                     ARGELGEATNSIIGYYSLKTNRMTMYDLTGVEEVGGGARATTAARINQVLSRPGAERT
                     VATIVHEATHQLAFNSGLQVRYADIPMWVSEGLAIYFETPDLESRTGWRNIGGVNRVN
                     LINYRKAAAGGSLLSLEDLLTDDKRFRDPSLAATAYAQAWALNYFLLKTRSEPYVKYL
                     QAQAAQSPLVTMDRDERLKQFQDHLGGDVTATEADFLRFMRSVN"
BASE COUNT          575 a         1146 c         1173 g          671 t
ORIGIN      
        1 cggcggcagc gagtgatgct ccagcaaata ggcccgcgtc ttggcatagc tggccgtgtc
       61 gacgtcgatc gagaacgtcg agagcggcgt gtccttgaca gcgagaaacg ggttctcatc
      121 gatataggca aacttatcgc ccccttcgcc gggtccgcgg ccttgatcga tccgggcttg
      181 ctccacggca tcgtcggcgg gcgtcatggg caagcccttc cctcccagca gcacgccctc
      241 ggccggcgcc gtttccgcag cgggctgagc cgaagctgtc ggcgcgggcg tcgtcgccag
      301 gttcatgggc gcctccgaag ccggtgcggc atagcgggag gcatcgatct cccgcagttc
      361 ccgctgcacg gctggagaag cagcatcacc ggcgggccca tgcactcctt cggcgccgca
      421 gcccaagatt gccgcagccg caggaattat catcaccagc gagagaaaac gggcgcgcac
      481 catgagcgga ctccttgaac caagggatga ccgaccgcat cacgccacgg aaccgcggct
      541 gcggctggat atcggcgaat aggagacgac gcacccagcg ccgccgattc atggagccaa
      601 tgaactcgaa tcctattccc gctttccagc aaagaaaaca aactaaactg cgcaaatggc
      661 gatcgtgcag cgactagccc gaactccact cgccacatgg ctagcggcgg cagttgcgct
      721 ggctgcgatc tgggcggcag cgccggacgc ggcgcggccc gcaatgatga actcggcgat
      781 tctggccggc gcggcgacgg cgatcgcgct gccgctcggg acgctgctgg cggtgctcat
      841 cacccgcttc gatttgcccg gccggcggtt ggccgcagcc tgtcttggcg tcctgctgtt
      901 tctgccgctc tatgtgcagc ttgcggggtg ggatgcggct gtgggcaaga tcgggtggct
      961 gaccttggcc tatggtcaca tggatgagcc gttcctggcg ggcatgcggg gagcggtttt
     1021 cgtgcatgcg atggcggccg tgccgtgggt cgcgctcgtc gtcggtctgg gcttgtcgca
     1081 agtcgatcca gctcaggaag aggcagcgct tctgatcctg tcgccaatcg aggtgctgtt
     1141 tcgcgtcacg ttgcgtcagg ctgtgccctt catcctcgca gcagccgtgc tgacggtcgt
     1201 cgccacgacg agcgaaatga ccgtcaccaa catcttcctg atcaacccaa tggagcagac
     1261 cttcaccgag cagttctaca tgacctttgc gctgcaagcc gacgccgggg aggcggcgcg
     1321 aagcgtatgg ccggcagtgg ctgggctggg cgtgctcatt acgctgtcgc tttggatggc
     1381 gattcacgcg gcccgtcgga cgcactggtt ttctgcacaa cgaccggtga cattcaccgc
     1441 cggaacgctg cgtcctttcg ctgcggcgct aatgtggctg ctgattgcgg tgctgttggc
     1501 aatcccgatt ggcagcctga tctcgaaggc cggctttgtc gtcgtccacg aggggagcaa
     1561 gcgggtgcag tcatggtcgc ttgccaagtg cgtggagcag gtggcgcagg cgccggcaca
     1621 attctcgttc gagttccgct ggacgatcat cacggcgctc ggagcggcga cgtgtgccct
     1681 ggcggcagcc gtcgcgcttg cctggccagc gcggcgcggc ggctggcggg caatgccggc
     1741 aatcgcggcc gtggtgctgg gtctgacgat cccggggccg ctggtgggcg tttcgctcat
     1801 ttgggtcttc aatcgctcgc tttggccgaa tgtctttgat ccgcttgtct atctttacga
     1861 tcgcacgccg gtggtggtga tgttcgcgca ggctatacac gccttgccga tcactctcct
     1921 cgtgtgctgg tattcgttcc gcacgctcag cgacgacgtc ctggctgccg cggcgctcga
     1981 cggcgcaccg ccgcggcaag tgctctggcg gattgccctg ccgctgcgct ggcaggcgct
     2041 ggcgggtgcg tggctggcgg catttgccat cgcggcgggc gatgtggcct gggcacagct
     2101 tgtccggccg gcgggaatgg acctgatcca gcgccgcgtc ctcgggctgg tccattcggg
     2161 cgtcgatgag caggtggcgg ccatttcgct gatcaacgtc atcgcctacg ccgtgctgac
     2221 cggcctgatc ttgtggctcg tcggaccgcg tcgcaattgg cgtcgagttg cagccgcagg
     2281 cgacctataa tcgagacatg agccgatcca ttgctgtttt ggggttgttg ctggccgctg
     2341 ccgctccagc gctgggcctg gaatacgtca cgatccaaca gaacggcagg cagaccgaac
     2401 tggcgggcaa gctcgcagtt gaagccgagg acggcggcgt gatgctcctg acgcccgatg
     2461 gcgtcctctg gccgctgccg aaggaggaaa tcgtcagccg ccggtcggac gaaaagccct
     2521 tcgttccgct ttcggccgag gaattgtcgg ccaagctgac ggctgagctg ccggcgggat
     2581 tcaagatcca tcagacaaag aattacctga tctgctacaa cacgtcgccg gcatacgccc
     2641 agtgggtcgg cgggctgttc gagcgcttga acggagcgtt ctacaactac tggacccgcc
     2701 gcggcgtcga gatgcgcccg ccgcagtttc cgctcgtcgc gctcgtcttc gacagcaaat
     2761 cgtcctacgc ccagcacgcc cgcggcgagc tcggcgaggc gaccaactcg atcatcggct
     2821 attacagtct gaagacgaac cggatgacga tgtacgatct gacgggcgtc gaggaggtcg
     2881 gcggcggcgc gagggcgacg accgcggctc gcatcaatca ggtgctctcg cgtcccggcg
     2941 ccgagcggac cgtcgcgacg atcgttcacg aggcgacgca ccagctcgcc ttcaacagcg
     3001 gcctgcaggt gcggtacgcc gacattccca tgtgggtcag cgaagggctg gcaatttact
     3061 tcgagacgcc cgacctggaa agccgcaccg gctggcggaa catcggcggc gtcaaccgcg
     3121 tcaatctcat caactatcgc aaggcggctg ccggcgggtc gctcctctcg ctcgaggatc
     3181 tgctgaccga cgacaagcgt ttccgcgacc cgtcactggc cgcaaccgcc tacgctcagg
     3241 cctgggccct caactacttc ctgctgaaga cccgcagcga gccgtacgtg aaatatctcc
     3301 aggcccaggc cgcgcagtca ccgctggtga ccatggatcg ggacgagcgc ctcaagcagt
     3361 tccaggacca tctgggcggc gatgtgacgg ccactgaggc ggattttctg cggtttatgc
     3421 gaagcgtgaa ctgaccgctt caccttgacg ccgctggcgc ctggccgcta tagtccgcgg
     3481 ccttttctac ccgattcagg ctcccccgga agcgccgttc cgtgctgcgc acctgccacg
     3541 ccctgggcct ggttctcgcg ctcat
//
LOCUS       DASUGB010000080         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1399466, whole genome shotgun sequence.
ACCESSION   DASUGB010000080 DASUGB010000000
VERSION     DASUGB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399466"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="rpoD"
                     /locus_tag="VFV87_01545"
     CDS             <1..822
                     /gene="rpoD"
                     /locus_tag="VFV87_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX5102463.1"
                     /translation="LRDLMTMTLESPKSLRERCHAVRKHFVEYELAKRELSSGNLRLV
                     VSIAKKYRNRGLTFLDLIQEGNTGLMRAVDKFEYRRGFKFSTYATWWIRQAITRAIAD
                     QARTIRIPVHMIDVLSKLRNLQKQLLQQYGREATTEEVAELSGYPLEEVRRVLDIGRH
                     PVSLDRPVGDSEDSSFGEFLEDQASDNPLNNANQCILRQKIDSLLKTLTWREREIIRL
                     RYGLQDGYTYTLEEVGRIFKVTRERVRQIEAKAVRKLQNPVRSKHLEGFLKPSAA"
     gene            1032..1733
                     /locus_tag="VFV87_01550"
     CDS             1032..1733
                     /locus_tag="VFV87_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEX5102464.1"
                     /translation="MPSLTESLRTLHRIHKQLTDLRDRLERGPRQVRIAEGTVKKSES
                     EVTQAKDAFKQAKMACDEKQLQLKQREARLVDLQGKLNQAQSNKEYQLLKDQMAADKQ
                     ANSVLADEILEALDKLDQLQAHIKTADDNRAKTKDELVKVKKRVEDQHQGLESEFGRV
                     SAELTAAEELLQGEFKDNYLRLARSMGEDSLAPAEGECCGGCSQTLTVQMLNLLSLDK
                     PVFCKNCGRMLYLPE"
     gene            1910..2674
                     /locus_tag="VFV87_01555"
     CDS             1910..2674
                     /locus_tag="VFV87_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5102465.1"
                     /translation="MATTLAVVELWPAIHGLSPRSPLAGIARRRFAGKTLLEWLVRRV
                     SDAISLDQIVALPGNDARSRDLVAHCPPDISVLHSSADDPLGRLSDAVREIPCTGVVR
                     INICHPFVDPDLIDRLVAAAADSGRDYAGYALADGRPAIESQLGVFAEWCSAAAILRA
                     DRLAESADDRAEATRFLYSRPDLFALKWIPAPRQLDKGDLRLAIHDEEDWEHVQLILD
                     ALGPESLDWQYIASLLDRQPMLRARMADRNRGLLTA"
     gene            2699..3127
                     /gene="aroH"
                     /locus_tag="VFV87_01560"
     CDS             2699..3127
                     /gene="aroH"
                     /locus_tag="VFV87_01560"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5102466.1"
                     /translation="MLCRGVRGATTADENSREAILTATRQLLALMIRVNGIKPEDVGS
                     AIFSLTRDLDAEFPALAARQLGWLDVPLLCTYEVDVPGSLRRCVRVLIHWNTDKPQQQ
                     IAPVYLKDAQKLRPDLCKLPPVDFAELERWISEQMTQFQK"
     gene            complement(3299..>4707)
                     /locus_tag="VFV87_01565"
     CDS             complement(3299..>4707)
                     /locus_tag="VFV87_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5102467.1"
                     /translation="NATSLLLNPGQHEAVVVFVNSPPGKDREVRIVSCDLKAGKVNSD
                     VPWMTSFTPVDLSPSGELLACMPDQFTRNASEKNKIAIMRREGQQLTPVIRWSMGEGL
                     EFAKKFEQVHFLGEDRLLAFSAIGGSVTLFQIDQAKALWSVKTAQHNTPTLSANRAQF
                     AAVIDGGIGIFNAATGDTLARISTETPLQRGTLSFSPDGKRLAHLTSAVLLVWDLTTG
                     KQIHEVWFPTIQSGKSLDWCGEGFVLVDRSQLVDLDKRIVLWEYEFTGRGQPLAGYLG
                     GRFWLVASGSDHVHQLFSPTIPDASAVAKSQQLTADGVLALKPGAQVSVQVNLPAHGG
                     ELQKVTQELTNSLTKLGFIVVAGAPLTLEATITDDGKESVDYRGFGFGRRGSDKVEVQ
                     KYTSNVTLKENGKVIWTAGAHYGAPHMVRQKDGQTIQQAVDEQRGNPVDFFAHVRIPR
                     QMARHEADGAYGRSKLAP"
BASE COUNT          841 a         1552 c         1458 g          856 t
ORIGIN      
        1 ctgcgcgatc tgatgacgat gactctcgaa agccccaaga gcctgcgcga acggtgccac
       61 gcggtgcgca agcactttgt cgagtacgag ctggccaagc gcgaactctc cagcggcaac
      121 ctgcggctgg tggtgtcgat cgccaaaaag taccgcaacc gcggtctgac gttcctggac
      181 ctgattcagg aagggaacac gggcctgatg cgggccgtcg ataagttcga gtaccgccgc
      241 ggcttcaagt tcagcacgta tgccacgtgg tggattcgcc aggcgatcac ccgggcgatc
      301 gccgaccagg cccgcacgat ccgcatcccg gtgcacatga tcgacgtgct cagcaagctg
      361 cggaacctgc agaagcagct tctgcagcag tacggccgcg aggcgacgac cgaggaggtc
      421 gctgagctgt cgggctatcc gctggaggaa gtccgccgcg tgctcgacat cggccgccat
      481 ccggtcagcc tcgaccggcc ggtcggcgac agcgaggact caagctttgg cgagttcctc
      541 gaagaccagg ccagcgacaa cccgctgaac aacgccaatc agtgcatcct gcggcagaag
      601 atcgacagcc tgctgaagac gctcacctgg cgcgagcggg agatcatccg cttgcggtac
      661 ggccttcaag acggctacac ctacacgctg gaagaggtcg gccggatctt caaggtcacc
      721 cgggaacgcg tccgccagat cgaggccaaa gcggtccgca agctgcagaa ccccgtgcgg
      781 agcaagcact tggaagggtt cctgaagccg tcggctgcct gattctcgcg aggccgcgga
      841 tccgctcgtt cccaagctcc gcttgggaac gcacgcccgc gaagctctgc ttcgccctcg
      901 ggaagcggag cttccagact ggcgtaccca agcaggggct tggggaccag aggtgcgcgc
      961 ctttggctgc cggaaatgcg ttttccagcc gcccgtcctt ggatgggcgg cttttttcgt
     1021 ataatggcag catgcccagc ctgaccgaat cgctccgcac gctccaccgc atccacaagc
     1081 agctcaccga cctgcgcgat cggctggagc gcggtccgcg gcaggtgcgg atcgccgaag
     1141 gaaccgtcaa gaagtcagaa agcgaagtga cccaggcaaa ggatgccttc aagcaggcga
     1201 aaatggcctg cgacgagaag cagctccagc tcaagcagcg cgaggcgcgg ctcgtcgatt
     1261 tgcagggcaa gctcaaccag gcccagagca acaaggagta tcagcttctc aaggaccaga
     1321 tggccgccga caagcaggcc aacagcgtgc tggccgacga gattctcgag gccctcgaca
     1381 agctcgacca gctccaggcg cacatcaaaa cggccgacga caaccgcgcc aagaccaagg
     1441 acgaactggt caaagttaaa aagcgggtcg aggaccagca ccaggggctg gagagcgaat
     1501 tcggccgcgt cagcgccgag ctgacggcgg cggaagagct gcttcagggc gaattcaagg
     1561 ataattatct gcggctggcg cggtcgatgg gcgaggactc gctcgccccg gccgaagggg
     1621 agtgctgcgg cggctgctcc cagacgctga cggttcagat gctcaacctg ctgtcgctcg
     1681 acaagccggt cttctgcaaa aactgcgggc ggatgctcta cctgcctgag tgaactcggc
     1741 ccgcacatct gccgtacagc ggatgtacga tcgcggcaac ccgttgctag catgcggcaa
     1801 tccgcatttg cctgcaggtc aaattgacgc tggcaacccg cgcccgctaa attgaatgga
     1861 acattgtctc atcgagcgct gcacgccagc gcgcacggag ggtctacgga tggcgacgac
     1921 attggcggtg gtcgaactct ggccggcaat ccacggactt tcgccccggt cgccgctggc
     1981 gggaatcgcg cggcggcggt tcgcgggtaa gacgctgctg gagtggctcg ttcgccgcgt
     2041 atccgacgcg atttcgctcg accagatcgt cgccctcccc ggcaacgacg cgcgctcgcg
     2101 agacctggtc gcacattgcc cgcccgatat ctcggttctc cattcgtccg ccgatgatcc
     2161 gctgggccgg ctgtccgatg ccgtccgcga aatcccctgc acgggcgtcg tgcgaatcaa
     2221 catttgccat ccgttcgtcg atcccgattt gatcgaccgt ttggtagccg cggcggcgga
     2281 ctctggccgc gactatgccg gctatgccct ggccgatggc cggccggcga ttgagtcgca
     2341 gctcggcgtc tttgccgaat ggtgctccgc ggcggccatt ttgcgggccg accggctcgc
     2401 agaatccgcc gacgaccgcg cagaggcgac gcgttttctc tattcgcggc ctgacctgtt
     2461 cgcgctcaag tggattcccg ctccacggca gctcgacaag ggcgatctgc ggctggccat
     2521 tcacgacgag gaggattggg agcacgtgca gctgatcctc gacgccctcg gccccgaatc
     2581 gctcgactgg caatacatcg cctccctcct cgaccgccag ccgatgctgc gggcacggat
     2641 ggccgaccgg aaccgcgggc ttttgaccgc ctagtgcccg ccacgctatg ctagtcgcat
     2701 gctctgccga ggcgtacgcg gcgcgacgac tgccgatgag aattcgcggg aagcgatcct
     2761 gaccgcgacg cgccagcttc tcgcgctgat gatccgcgtc aacggcatca agccggagga
     2821 cgtcggcagc gcgatcttca gcctgacgcg cgacctggac gccgagtttc cggccctggc
     2881 cgcccgccag ctcggctggc tcgacgtgcc gcttctttgc acctacgagg tcgatgttcc
     2941 cggcagcttg cggcggtgcg tccgcgtgct gatccactgg aacaccgata agccgcagca
     3001 gcaaatcgcg ccggtctatc tcaaggacgc ccagaagctt cgccccgacc tctgcaagct
     3061 cccgccggtc gattttgccg aactggagcg ctggatttcc gagcagatga cgcagtttca
     3121 gaagtagtcc agatgcggag cgtgtcggtc gtttcggacc taattggctg aaaccagtct
     3181 ttttctgggc acgcctgcag ccaggcgaca agcccttcat tatagcgacg tacgcgtttc
     3241 acattgccct cctgtcctcc tgttccccct gttccgtccc ctgttccctg tccccttcct
     3301 acggcgccag cttcgacctc ccatatgctc cgtccgcctc gtgccgtgcc atctggcggg
     3361 gaatgcggac gtgggcgaag aaatcgaccg ggttgccgcg ctgctcatcg acagcttgct
     3421 ggatggtctg gccgtccttc tgccgcacca tgtgcggagc gccgtagtgc gctcccgccg
     3481 tccagatcac tttgccgttt tccttgagcg tcacgttgct cgtgtatttc tgcacctcga
     3541 ctttatcgct gccgcgccgc ccaaacccga agccgcggta atcgaccgat tcttttccgt
     3601 cgtcggtgat cgtcgcttcc agtgtcagcg gcgcgccggc cacaacaatg aaacccagct
     3661 tcgtgaggct gttggtcagc tcctgcgtca ctttctgcaa ttcgccgcca tgagccggca
     3721 ggttgacttg cacgctcacc tgcgccccgg gtttaagggc caacacgccg tcggctgtga
     3781 gctgctgact tttggcgacg gcgctggcat cgggaatcgt ggggctgaag agctggtgca
     3841 cgtggtcgga gccgctggca accagccaga accgcccgcc cagatagcct gccagcggct
     3901 ggcctctgcc ggtgaactcg tactcccaaa gcacgatccg cttgtcgaga tcgacgagct
     3961 gcgagcgatc gaccagcacg aagccttcgc cgcaccaatc gagcgacttg cccgactgga
     4021 tcgtggggaa ccagacctcg tgaatttgct tgccggtcgt caggtcccag acgagcagca
     4081 ccgccgaggt cagatgcgcc agccgcttgc cgtcggggct gaacgaaagc gttccgcgct
     4141 gcaacggcgt ttcggtgctg atccgggcta gcgtgtcgcc cgtggccgca ttgaagatcc
     4201 cgatgccgcc gtcgatgacc gcggcgaatt gcgcgcggtt ggcgctgagc gtcggcgtgt
     4261 tgtgctgggc cgtcttgacc gaccacagag ccttcgcctg gtcgatttga aacagcgtca
     4321 cgctgccgcc gatggcacta aacgccagca gccggtcttc gcccagaaag tggacctgct
     4381 cgaacttctt ggcaaattcg agcccttcgc ccatgctcca gcgaatgacg ggcgtcagtt
     4441 gctggccctc gcggcgcatg atggcgatct tgttcttctc gctcgcgttc cgcgtgaact
     4501 gatcgggcat gcaggcgagc agttccccgg aaggcgacag atcgactggc gtgaagctgg
     4561 tcatccacgg cacgtcgctg ttgaccttgc ccgccttcag gtcgcagctc acgatccgca
     4621 cttcccggtc cttgcccggc gggctgttga cgaagaccac gaccgcttcg tgctggccgg
     4681 gattgagcag caagctcgtc gcgttct
//
LOCUS       DASUGB010000081         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_10534,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000081 DASUGB010000000
VERSION     DASUGB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10534"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1872
                     /locus_tag="VFV87_01570"
     CDS             127..1872
                     /locus_tag="VFV87_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HEX5102468.1"
                     /translation="MTRTCCVQCGGRALAAAAILAFFPVVTFLASDALAGDAADKVQA
                     HLEAGEFGPAKAAAAAIGDAAARDRAFADIAAAQAGAGARRASLHSLHDISGDLSRSE
                     ALSRVARQPGFFGGARGGGVIADFDSLIDLITTTISPDSWDEVGGPGSIDSFEGGVFV
                     DPTGLLRRLPPETDRSLVVVRQAAALATHTTDPRRKSILRKISLQRLEREVQMLHALG
                     RDPDEAMQTLAGLKRIKYILVYPETGDIVLAGPAGDWRRDAEGRFVEADSSQPVVNLD
                     DLVVTLRNAYGDLGRFGCSITPRRENLAAAQEINDRWSKQPLKPSQREKWLSEFRDAV
                     GRQDIEIYGIDPRTRTGRVIVEADYRMKLVGLGLEEGTLGVTSYLDSIPVSADGKAPP
                     MSVLRWWFTLNYAALSATEDRNAFELKGPGVKVQSENELLTERGERVHTGKSDELNQQ
                     FAESFTNHFEQLAAKYSIYADLRNIFDLSLVAAVIHSHDLPGQTNWHMTHFGAEGAYE
                     PELGFAPAAVESIMNHKLVGGKHIIAAVSGGVTVDARELAARQAVKTDGYGLLKTERS
                     GAAPQNLPRGAWWWD"
     gene            complement(1947..>2397)
                     /locus_tag="VFV87_01575"
     CDS             complement(1947..>2397)
                     /locus_tag="VFV87_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102469.1"
                     /translation="SCGGTGSLPFAVKQAGITEIQAGGVIFMDAFYRQLCKIEQFRFA
                     LFLITTVVSRPAPERAIIDAGRKSMNIEITPPLVRCHPGIEVTRLSAEHGQLKLDPGA
                     GDLRIGDRLELIPGYGDLTTVLHNRFYVLQNDRLVDLWPLTARGRLE"
BASE COUNT          435 a          758 c          774 g          430 t
ORIGIN      
        1 ggcggcagga agccagagtc aaagtgaacc actacctaaa cgctccaaac ttgactctgt
       61 gttcgccgcc tgcgacacta tcagtcacac tccttctcag gggctttgag aagagaagag
      121 gttcttatga ctcgcacgtg ctgcgtgcag tgcggcgggc gggctctggc cgccgctgcg
      181 attctggcgt tcttcccggt tgtgacattc ctggcatcgg atgccttggc gggcgacgct
      241 gccgacaagg tccaggcgca tctggaagcc ggcgaattcg gtccggcaaa ggctgcggct
      301 gcggccattg gcgatgccgc ggcgcgcgac cgcgcattcg ctgacattgc ggcggcccaa
      361 gccggcgcgg gcgcacggcg ggcttcgctc cattcgctgc acgacatttc cggcgatctg
      421 tcgcgcagcg aagctttaag tcgtgttgcc cggcagccag gattcttcgg cggcgctcgc
      481 ggcggcggcg tcattgccga cttcgattcg cttattgact tgatcaccac gaccatctcg
      541 cctgactcct gggatgaagt cggcgggccg ggctcaatcg acagtttcga aggcggcgta
      601 ttcgtcgatc ccacgggatt gcttcgccgg ctgccgccgg aaaccgaccg ctcgctggtt
      661 gtcgtccgcc aggctgcggc gctggccacg cacacgaccg atccgcgtcg caagtcgatc
      721 cttcgcaaga tctcgctgca gcgtctggag cgtgaagtgc agatgctgca cgccctggga
      781 cgagatccgg acgaggcgat gcagacgctt gccggcctga agcgaatcaa gtacatcctg
      841 gtctatcccg agacgggcga catcgttctc gccggtcctg ccggcgattg gcgccgcgac
      901 gcggaaggcc gctttgtcga ggcggattcc agccagccgg tcgtgaatct tgacgatctg
      961 gttgtcacgc tccgcaacgc ctacggcgat ctcggccgct tcggctgctc gatcacaccg
     1021 cgccgtgaga atctcgcggc ggctcaggag atcaacgacc gctggtccaa gcaaccgctc
     1081 aagccgagcc agcgcgagaa atggctctcc gaatttcgcg atgcggtcgg acgacaggat
     1141 atcgagattt acggcatcga tcctcggacg cgcacggggc gcgtcatcgt cgaagccgac
     1201 taccggatga agctcgtcgg cttggggctg gaggaaggga cgctgggggt cacgagctat
     1261 ctcgattcga ttcccgtttc ggcggacggc aaggcgccgc cgatgagcgt gctacggtgg
     1321 tggtttacgc tgaattatgc ggcgctatcg gccaccgagg accgcaacgc cttcgagctc
     1381 aagggcccag gcgtgaaggt gcagagcgag aacgagctgc tgacggagcg cggcgagcgc
     1441 gtgcataccg gcaagagcga cgagctgaat cagcagttcg ccgaatcgtt cacgaatcac
     1501 tttgagcaac tcgccgcgaa gtattcgatc tacgccgatc tgcgaaacat cttcgatctg
     1561 tcgctggtcg ccgccgttat tcacagccac gatttgccgg gccagaccaa ctggcacatg
     1621 acacacttcg gcgctgaagg cgcttacgaa cccgagctcg gcttcgcgcc agcggcggtc
     1681 gaatcgatca tgaatcacaa gctggtgggc ggcaaacaca ttattgccgc ggtcagcggc
     1741 ggcgtgacgg tcgatgcccg cgagcttgcc gctcggcagg cggtcaagac cgatgggtac
     1801 ggcctgctga agaccgagcg cagcggcgcc gccccgcaga acctgccccg cggcgcctgg
     1861 tggtgggatt gaccacaaag cagcgaccag cggtcgcggt tccgatccga ttctgccgcg
     1921 accactggtc gcggctttgg cggcagctac tccaaccgcc cgcgcgccgt gagcggccac
     1981 agatcgacca gccggtcgtt ttgcagcacg tagaagcggt tgtgcagcac ggtcgtcaga
     2041 tcgccatagc cgggaatcag ctccagccga tcgccgatcc gcaagtctcc cgcgccaggg
     2101 tcgagcttca gttggccatg ctccgccgat aaccgcgtga cctcgatgcc tggatggcag
     2161 cgcaccagcg gcggcgtgat ctcgatgttc atcgacttgc ggccggcgtc gatgatggcc
     2221 cgttccggcg ctggccggct gacgacagta gtaatcaaga atagggcaaa gcggaactgc
     2281 tcgatcttgc aaagctggcg atagaaggcg tccatgaaga tcacgcctcc cgcctggatc
     2341 tcagtgatgc ccgcttgctt caccgcaaag ggcagcgagc ccgtcccgcc gcaggag
//
LOCUS       DASUGB010000082         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1816943, whole genome shotgun sequence.
ACCESSION   DASUGB010000082 DASUGB010000000
VERSION     DASUGB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816943"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFV87_01580"
     CDS             <1..351
                     /locus_tag="VFV87_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102470.1"
                     /translation="KIGGDPLPRYDLFQWGGFLQQSGYATGQLIGESLKFARLTMYRR
                     ILRGTLLEGAYGGVTIEFSKIGNALVPGSPEGLLKSAALFVAADSPVGPLYLGYGHAK
                     DGNSSWYFYLGKPF"
     gene            639..983
                     /locus_tag="VFV87_01585"
     CDS             639..983
                     /locus_tag="VFV87_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102471.1"
                     /translation="MELEGEQIRLLLGGGTGKSVLHFNGKSYPFTVKAGSAGGVGATK
                     VTATGNVYFLKQLDDFAGMYTAVTAGAAAVKGAGRSQVQNDQGVFIDVRSKSEGVGLS
                     LGLAAISISFAK"
     gene            1195..>2134
                     /locus_tag="VFV87_01590"
     CDS             1195..>2134
                     /locus_tag="VFV87_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEX5102472.1"
                     /translation="MKNDKGRFASEARFGAGSKFVTTKNHRSMNLAPLPSLLIAVLVL
                     LIGTLTNRRVALLAKYNIPDPITGGLLFAVVASIAAAAAGFRLGIDQTLKPVLLLVFF
                     AGVGMCADLRLLKLGGKALALFVLVLFPYILVQNAVGIAGARLLDQHPIFGLVGGSIT
                     LVGGHGTGAAYAERFAQVNNLQSVMELSMTVATMGLIVGGILGGPVAQYLINRYEVRT
                     GGTAAANAPAVLEDAPVPMTSVAVMGALAGIFAAVIAGGWLGQRFSGAAITLPSFLWC
                     MLVGIAIRNLVPFTGIRCDDRAADLIAGVSLGLFLVM"
BASE COUNT          353 a          691 c          695 g          395 t
ORIGIN      
        1 aagatcggcg gcgatccgct gccgcgctac gacttgttcc agtggggcgg cttcctgcag
       61 cagtcgggct acgcgaccgg gcagctcatc ggcgagagcc tgaagttcgc acgcctgacc
      121 atgtatcgaa gaatattgcg aggcactcta ctggagggcg cctacggagg cgtgacgatc
      181 gagttcagca agatcggcaa tgcgctggtg cctggcagtc cggagggcct gttgaagtcg
      241 gcggcgctgt tcgtcgccgc cgacagtccg gtgggcccgc tgtacctcgg ctacggtcat
      301 gccaaggacg gcaattcgag ctggtacttc tatctcggaa agccgttctg acgccccccg
      361 tcgagtgcgc cgagtcattc ggctagcgaa agtcgctgcg ccgaaagtgc aaggccggct
      421 gccatagcgg ccgcactcag cgagccggga caactttgcc atggcgggtg caaggcgttc
      481 cgacatggct ggcgggatcc atcccgcgca ctcggcgact cacctttcag ctcgccaagc
      541 cgcagatgcc gactgcacca aagtccgatt gttctggcag ggagggcaag gtttcatcgc
      601 aatcggggaa gcgtcggctc agcaagccca gcgcaacgat ggaattggaa ggcgagcaga
      661 tccggttgct gctgggcggc gggaccggaa aaagcgtcct gcacttcaac ggcaagtcct
      721 atccgttcac ggtcaaggcc gggagcgcgg gcggcgttgg agcaacgaag gtcacggcga
      781 cgggcaacgt ctactttctg aagcaattgg acgatttcgc cggcatgtac acggccgtca
      841 ccgcaggggc ggcggcagtc aagggcgccg gccgttcgca agtccagaac gaccagggcg
      901 tcttcatcga cgtacgctcc aagtccgaag gagtcggcct gagccttggc ctggccgcca
      961 tctccatcag cttcgcgaag taatgcgcgc catgccttca gcaccacagc gcacagtcag
     1021 tcgcgcgggc agggcggtcc ctgaacgatt gcccaccccg cgtcagcaca ggtggtgagt
     1081 cgtcgcgcca ggccgtccgg cgggcgtgac tggccgccgc agtcgatcgc gccgggtcca
     1141 tgatggacct tggtccaata tcgagaccgc gccggcaatg acaacatcct gtcgttgaaa
     1201 aacgacaaag gccgattcgc cagcgaagcg cgctttggcg ccggctcgaa attcgtcaca
     1261 accaagaacc atcggtccat gaatctggcg ccgcttccct ccctgctcat tgcggtgctc
     1321 gtactgctga tcggcacgct gacgaaccgc cgggtggcgt tgctcgcgaa gtacaacatt
     1381 cccgatccca ttaccggcgg gctgctgttc gcggtcgtcg cctcgatcgc ggcggcggcg
     1441 gcgggctttc ggctgggcat cgaccagacg ctcaagcccg tgctgctgct cgtattcttc
     1501 gccggcgtgg gcatgtgcgc cgatctgcgc ctgctcaagc tcggcggcaa ggcgctggcc
     1561 ctgttcgtgc tggtgctgtt tccgtacatc ctggtgcaga acgcagtcgg catcgcgggc
     1621 gcccggctgc tcgaccagca tcccatcttt ggcctggtcg gcggttcgat caccctggtc
     1681 ggcggacacg gcaccggtgc ggcttatgcc gagcgctttg cgcaggtgaa caacctgcaa
     1741 tcggtgatgg agctttccat gaccgtcgcc actatgggcc tgatcgtggg cggcatcctc
     1801 ggcggccccg tcgcccagta cctgatcaat cgatacgagg tccggaccgg tggcacggca
     1861 gcggccaacg cgccggcggt gctggaggac gcgcctgtgc ccatgacgag cgtcgccgtc
     1921 atgggcgccc ttgccggcat cttcgccgcg gtgattgccg gcggttggct cggccagcgc
     1981 ttttccggcg ccgccatcac cctgccgagc tttctgtggt gcatgctcgt cggcatcgcg
     2041 attcgcaacc tcgtcccttt caccggcatt cgctgcgacg accgtgccgc cgatctcatc
     2101 gccggcgtat cgctggggct ttttctcgtg atga
//
LOCUS       DASUGB010000083         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1260935, whole genome shotgun sequence.
ACCESSION   DASUGB010000083 DASUGB010000000
VERSION     DASUGB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260935"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VFV87_01595"
     CDS             complement(<1..1430)
                     /locus_tag="VFV87_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102473.1"
                     /translation="MSLPLRSLCSLLFLSVPAMALAQAGFDPAARVAAVAPFLDEHTI
                     AVGHVDLKRIDPAAAIRTIGDLAPKDDLDFRKRLVEMEAGLTGVLKSLGEAGVTDVYL
                     VASIADIPQNPPFLVAPIKAGGNAQVAAEMLGKIIGLPAREEIGGAAVVAKDQAILER
                     LKALKPTPRAELAKAFEIAGDTAAQLIFSPTDDTRRVLREMLPRLPDEIGGGSGKMLA
                     DGVQWAVISANAPPKLSLDVRVQSKDEDSAAALRGIVMSAIQVVREKAQATEASAAER
                     EATSAVIRLVTPQLRGSQLVVSHVQDDADVQTLLKAFVPAVQAARTAAGRSQSLNNLK
                     QIALALHNYHDVYGHFPPPAILSKDGKPLLSWRVAILPFLEHQPLYEQFKLDEPWDSA
                     HNKKLIEKMPATLASASLTDAMRGQGLTTYLAPLLALPTNVSQESIFFSPEGTKMSKI
                     TDGTSNTIMVLEAHRDHAAVWTKPAD"
     gene            complement(1478..2953)
                     /locus_tag="VFV87_01600"
     CDS             complement(1478..2953)
                     /locus_tag="VFV87_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102474.1"
                     /translation="MQSVPIRALALLVAACAAPVFGQAPVAEDPNKPEMIELTVAPAP
                     EPRPALKYRLIPATAERTPGNAAPFYYRALLHLNNLPKDYWKPYDENLETWLSPDPGV
                     FPKDAVAKWLPSGAWRSQLEVAAYREYCDWDYRIQDLRGMEVIGFLLQETQDCRSLAR
                     VLRLQAHYEIMDGRPEDAIKTLRLGYQLAHDVAQPPLLINGLVGIACASLMNEELQLL
                     IDRGGPNMYWAIASLPQPLVSLRRAMEFEMGMPQQLFPFLKDAETAQRTPDEWRKLMI
                     EGLIGLQGVAGESQPELTGWQAELAAAAVVAKLYPIAKDALIASGMDRQWVEEMPVGQ
                     VVAIHTARSTEYVFQEFHKLSLLPYGEAMRRSPKIRDRLIQEGHLGPGIRGPGGLPIA
                     ALLLPAVEGVRHAEIRIARNFAALQAIEAMRMHAAETGKLPATLSEVTVVPVPDNPAT
                     SEPFPYQYDAATNTATLEVPASPGQQARHEAKRYVIRLVGN"
     gene            complement(2977..>3226)
                     /locus_tag="VFV87_01605"
     CDS             complement(2977..>3226)
                     /locus_tag="VFV87_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102475.1"
                     /translation="TSTAEAPRPAPRAVTNPQVPADNYLRTREVALRMGVDALGSRRS
                     AAGEATPPMTYFDWLSGFGGTTSSAQPGEPSDVLLPQM"
BASE COUNT          540 a         1021 c         1065 g          600 t
ORIGIN      
        1 aaatccgcgg gtttggtcca tacggccgca tggtcgcggt gcgcttcgag gaccatgatc
       61 gtgttgctcg taccgtcggt gattttgctc atcttggtcc cctcggggct gaagaagatc
      121 gactcttggg acacgttcgt cggcaaggca aggagcggag cgagatatgt cgtcagccct
      181 tggccccgca tggcgtcggt taaggaggcc gaggcaagag tcgccggcat cttttcaatt
      241 agcttcttgt tgtgcgcgct atcccagggc tcgtcgagct tgaactgctc gtacagcggc
      301 tgatgttcga gaaacggcag aatggcaacc cgccaggaga ggagcggctt gccgtctttg
      361 gagagaatgg ccggcggagg aaagtgccca tagacgtcgt gatagttatg cagtgccagg
      421 gcgatctgct tgaggttgtt caagctctgg ctgcggccgg ccgcggtgcg ggcggcctgc
      481 accgcgggca cgaacgcctt aagcagcgtc tgcacgtcgg catcatcctg gacgtggctg
      541 acgacgagtt ggctccccct gagctgcggc gtgaccaggc ggatgactgc actggtcgct
      601 tcgcgctccg cggcgctggc ttcggtcgct tgggccttct cgcgaacgac ctggatggca
      661 ctcatcacaa tgccccgcag cgcggccgcc gagtcctcgt ccttggattg cacgcggacg
      721 tcaagcgaca gcttcggcgg cgcgttggca gagatcaccg cccactgcac gccatcggcc
      781 agcattttgc ccgagccgcc gccgatttcg tcgggcaaac gcggcagcat ttcgcggagg
      841 acgcggcggg tgtcgtcggt cggcgagaaa atgagctgag ccgccgtgtc gccggcgatc
      901 tcaaacgcct tcgccagttc cgcccgcggc gtcggtttga gcgccttcag ccgttcgagg
      961 attgcctggt ccttggcgac gaccgccgct ccgccgattt cctcccgggc aggcaggccg
     1021 atgatcttgc cgagcatctc ggctgcgact tgcgcattgc cgcccgcctt gatcggcgcg
     1081 acgaggaacg gggggttttg cgggatatcg gcaatgctgg ctacgagata cacgtccgtg
     1141 acgccggcct cgccgagcga tttgagcacg ccagtcagcc ccgcctccat ctcgaccagt
     1201 cgtttacgaa agtcgagatc gtccttcggc gccagatcgc cgatcgtccg gatcgccgcc
     1261 gccggatcga tccgcttgag atcgacgtga cccaccgcga tcgtgtgctc atccagaaat
     1321 ggcgccaccg ccgccacgcg ggcagccgga tcgaacccgg cttgggcgag cgccattgcg
     1381 ggaacggaga ggaacagaag ggaacaaagg gaacgcagcg ggagggacat gagcggctcc
     1441 gtggaggttg gtggttctag caattttcag tttttggtca atttcctacc aggcgaatca
     1501 cgtatcgctt cgcttcatgg cgcgcctgct ggccggggct ggcggggact tcgagcgtcg
     1561 ccgtgttcgt cgcagcgtcg tactggtagg ggaacggctc gctggtagcc ggattgtcgg
     1621 gcacgggaac gacggtcacc tccgagagcg tggccggcag cttgcccgtt tcggccgcgt
     1681 gcattcgcat cgcctcaatc gcctgcagcg cggcaaagtt gcgggcgatg cggatttccg
     1741 cgtgccgtac accctcgacc gccggcaaga gcagcgcggc gatcggcagt cctcccggcc
     1801 cgcgtatgcc cgggccgaga tggccttctt ggatcaggcg gtcgcgaatc ttcggcgagc
     1861 ggcgcatcgc ctccccgtac ggcaagagtg aaagcttgtg aaactcctgg aagacgtatt
     1921 cggtcgagcg cgccgtgtgg atcgcgacga cctgacccac cggcatctct tcgacccact
     1981 gccgatccat gccgctggcg atcagcgcgt ccttggcgat cgggtagagc ttggcaacaa
     2041 cggcggctgc ggcgagctcg gcttgccagc ccgtcaattc cggctgcgac tccccagcca
     2101 ccccctgcag gccgatcaga ccctcgatca tcagcttccg ccattcgtcg ggcgttcgct
     2161 gggccgtttc ggcatccttc aggaagggga agagctgctg cggcattccc atctcgaact
     2221 ccatcgcccg ccgcaggctg accagcggct gcggcaggct ggcgatggcc cagtacatgt
     2281 tcgggccgcc gcggtcgatc aaaagctgca gctcttcgtt catgagcgac gcgcacgcaa
     2341 ttccgaccag tccgttgatg agcaacggcg gctgcgccac gtcgtgtgca agctgatagc
     2401 ccaggcggag cgttttgatc gcgtcctccg gccggccgtc catgatttcg tagtgcgcct
     2461 gcagccgcaa cacccgggcc agcgaccggc aatcctgcgt ctcttgcagc aaaaagccga
     2521 tgacctccat tccacgcagg tcctggatgc ggtaatccca atcgcagtat tcgcgatacg
     2581 ccgccacctc aagctgcgag cgccacgcac cgctgggcag ccatttcgca acggcgtcct
     2641 ttggaaaaac gcctggatcg ggcgagagcc atgtctcgag gttttcgtcg tagggcttcc
     2701 agtaatcctt gggcaggttg ttcaggtgca ggagtgctcg gtagtagaac ggcgcggcgt
     2761 tgcctggcgt tcgctctgct gtcgcgggta tcagacgata tttgagcgcg ggccggggct
     2821 caggagcggg agcgaccgtc agctcgatca tttcgggctt gttcggatcc tccgccaccg
     2881 gcgcctgccc gaaaacaggc gcagcacatg ccgctaccag tagtgccaga gcgcgtatcg
     2941 gaaccgattg catgggtcaa accttggaag aagggtttac atctggggaa gcaacacgtc
     3001 ggacggctcg ccgggctgcg cggatgacgt cgtgccgccg aacccagaga gccaatcgaa
     3061 atacgtcatg gggggcgtcg cctcgccggc cgcagaccgc cgcgagccga gcgcatcgac
     3121 gcccatccgc agcgcgactt cgcgcgtgcg gagatagttg tcggccggaa cctgcgggtt
     3181 cgtgacggca cgcggcgcgg gccgcggagc ctcagccgtg ctggtc
//
LOCUS       DASUGB010000084         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1539720, whole genome shotgun sequence.
ACCESSION   DASUGB010000084 DASUGB010000000
VERSION     DASUGB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539720"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..>2007)
                     /gene="infB"
                     /locus_tag="VFV87_01610"
     CDS             complement(160..>2007)
                     /gene="infB"
                     /locus_tag="VFV87_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5102476.1"
                     /translation="RLKRRVTMKSHGSTAAPRKGKVTVELPCTVRSYSESTGVGSGQV
                     LKTLMGLGMMANINAPIPNDYLELLTTELGLDIEFKAATSLEEKLLARFESGEEDPAK
                     LEPRPPIVTVLGHVDHGKTSLLDKIIGINVVAGEAGGITQHIRAYAVPTKDGRTVSFV
                     DTPGHEAFTEMRARGANVTDIAVLVVAADDGVMPQTEEAISHAKAAEVPIVVAMNKID
                     LPGADEQKVLQQLATAGLLPSEWGGEVEVVRTSAVTGQGVETLLDTLLVTAELHEYKA
                     NADKPARGMCLEAEQEPGRGVIAKLMVQDGTLKEGDVIVCGGGYGRVKAMFDTLRPRQ
                     KVKEAGPSTPVNVTGLDVAPNAGDRFYVLDDIAEARALADQRQLESRQEALGGITTKV
                     SFEDFQQQLAEGRLGQDEDIATLNLIIRADVRGSIEAILKELEKFEHPEVKVKVLQAS
                     VGGITVADVTLAHASGAVVVGFNVIPDEAARSLADARQVEIRRYDIIYKLTDDIKLLV
                     EGKLKPEERVVEMGTALVKQVFTISRVGSVAGCQVIRGSIERGCRIRINRDSRTIGDY
                     AIDTLKRVKDDVKEVSRGMECGIKLAGFNDIKEGDVLDAYKIEEVARTL"
BASE COUNT          308 a          640 c          633 g          426 t
ORIGIN      
        1 ggaaactttt tactgaggtt gtaaacccgc atcgccagat cgtaagcctt gcgatagaca
       61 gtcaagtcct tggcggattg aatacgcacg gcacgatctc cgaccctctt gtggcgacaa
      121 tctgcccgcg cctctgtcct ctgtcttctg tcttctcccc tacagcgtcc gggccacttc
      181 ctcgatcttg tacgcatcca gcacgtcgcc ttccttgatg tcgttgaagc cggcgagctt
      241 gatgccgcat tccatgccgc gcgagacttc tttcacgtcg tctttgacgc gcttcagcgt
      301 gtcgatcgcg taatcgccga tcgtgcggct gtcgcggttg atgcggatgc ggcagccgcg
      361 ttcgatcgat ccgcggatga cctggcagcc ggcgacggag ccgacgcggc tgatggtgaa
      421 gacctgcttg accagggcag tgcccatttc gaccacgcgc tcttcgggct tgagcttgcc
      481 ttcgacgagc agcttgatgt cgtcggtgag cttgtaaatg atgtcgtagc ggcggatttc
      541 gacctggcgg gcatcggcca ggctgcgggc ggcttcatcg ggaatgacgt tgaagccgac
      601 gaccaccgcg ccggaggcat gggccagcgt cacgtcggcg acggtgatgc cgccgaccga
      661 ggcctgcagc accttgacct tcacctcggg atgctcgaac ttttcgagct ccttgaggat
      721 cgcctcgatc gagccgcgga catcggcccg gatgatcaga ttgagggtgg cgatatcctc
      781 atcctgtccc agacggccct cggccaactg ctgctggaaa tcctcgaacg aaaccttcgt
      841 ggtgatcccg ccgagggcct cttgccgcga ttcgagctgc cgttgatcgg ccagggcacg
      901 ggcctcggca atgtcgtcga gcacgtagaa cctgtcgccc gcgttgggcg cgacatccag
      961 gcccgtcacg ttcacgggcg tgctggggcc agcctccttg accttctgcc gcgggcggag
     1021 cgtatcgaac atcgccttca cgcggccata gccgccgcca cagacgatca cgtcgccttc
     1081 cttcagcgtg ccgtcctgca ccatcagctt ggcgatgacg ccgcggccgg gctcctgctc
     1141 cgcttcgagg cacatgccgc gggccggctt gtcggcgttg gccttgtatt cgtgcaattc
     1201 ggcggtgacc agcagcgtat cgagcagcgt ctcgacccct tggccggtga cggcgctggt
     1261 gcggacgact tccacttcgc cgccccactc gctgggcaac aggccggcgg tggcgagttg
     1321 ctgcagcacc ttctgctcgt cggcgccggg caggtcgatc ttgttcatcg ccacgacgat
     1381 tggcacttcc gccgccttgg cgtggctgat ggcttcttcg gtctgcggca tgacgccgtc
     1441 atcggcggcg acgaccagca ccgcaatatc ggtcacattg gcgccgcggg cccgcatttc
     1501 ggtaaaggct tcgtgacccg gcgtatcgac aaaactgacc gtgcggccgt ctttcgtcgg
     1561 tacggcatag gcgcggatgt gctgggtgat tccgcctgct tcgccggcga cgacgttgat
     1621 gccgatgatc ttgtcgagca gcgacgtttt gccgtgatcg acatggccga ggacggtgac
     1681 gatcggcggc cggggctcga gcttcgccgg gtcttcctcg ccggattcaa accgcgccag
     1741 gagcttttcc tccagcgacg tggcggcctt gaattcgata tccaggccca gttcggtcgt
     1801 cagcaattcc agatagtcat tggggatggg cgcgttgatg ttggccatca tgcccaggcc
     1861 catcagcgtc ttgaggacct ggccggagcc gacgcccgtc gattcgctat aggaacgaac
     1921 ggtgcagggc aattcaacgg tgactttgcc tttgcgcgga gcagccgtgc tgccgtggct
     1981 cttcatcgtg acgcggcgtt tgaggcg
//
LOCUS       DASUGB010000085         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_566189, whole genome shotgun sequence.
ACCESSION   DASUGB010000085 DASUGB010000000
VERSION     DASUGB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566189"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFV87_01615"
     CDS             complement(<1..361)
                     /locus_tag="VFV87_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5102477.1"
                     /translation="MFSRYWITALSLVLIPLAVVAEDAKDDFKRERKEGDTSKDSLEG
                     KSPPALQVENWLNTDGKEIDLAGLKGKVVVLDFWGTWCGPCRAAMPHLKELYAKHNED
                     GLVVIGVHTTNQGEEMAD"
     gene            623..868
                     /locus_tag="VFV87_01620"
     CDS             623..868
                     /locus_tag="VFV87_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102478.1"
                     /translation="MAPSRLRVYHGPEEAAFDASPATIPLHDQVKLTLGEVLPLILDA
                     VKSRRSWVDDFADDEISISTDLYEVLLAYQHFRRPSA"
     gene            1097..2101
                     /locus_tag="VFV87_01625"
     CDS             1097..2101
                     /locus_tag="VFV87_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5102479.1"
                     /translation="MSSYDLATQKHDRRSNIDDLIQTIKETADKLAADGSTRGDLKIL
                     SRTLRELRYAFKVFAPYRRKRKVTVFGSARTPPEHPAYQQAVAFGRAMAEAGWLVVTG
                     AAAGIMEAGHVGAGRDYSMGLNIMLPFEQSANPVIAGDIKLVHMKYFFTRKLMFVKEC
                     DAVVCSPGGFGTLDEAMEVITLLQTGKRDMIPVVLLDPPGSGYWQGLNDFIHNQLLRN
                     RLISPEDLHLFKLTDDPEAAKGEVLQFFRVYHSMRYVKNELVIRVQRPLDAPFLEAIN
                     TGFSDLLVTGRFEQREALPLERDETDLAYLPRLVFQFNRRNFGRLRLLIDAINRGSVT
                     "
BASE COUNT          429 a          661 c          670 g          416 t
ORIGIN      
        1 agtcggccat ctcctcccct tggttcgtgg tatggacgcc aatcacaacg agtccatcct
       61 cgttgtgctt ggcataaagc tccttgaggt ggggcatcgc cgcgcggcag ggaccgcacc
      121 aggttcccca gaaatcgagc acgacgacct tgcccttgag acctgcgagg tcaatctcct
      181 tgccatccgt gttcagccag ttttcgactt gtaaagccgg cggtgacttc ccctccaggg
      241 aatccttgga cgtatcccct tccttgcgct cgcgcttgaa gtcgtccttg gcgtcctcgg
      301 ccacgaccgc cagtggaatc aaaacgagtg acaatgcggt gatccaatag cggctaaaca
      361 tgagcggcac tccaaaaaaa gacgaaagga aagacaggcg cagcagttca atgaacgaac
      421 caaggtaatg gattaccgcg gctggcggca atcaggcaac agagtcggca gagggatcag
      481 aaaatcgcca aagttctgca gccaaccctg ccgataagta ggagatagcg aggtcggttc
      541 gcgcacctgc ttgcgctgct agcgagtggc agacgatttc gctgatcgct tggaaggact
      601 ctcacgccag gaaggatccg gcatggcccc gagccgcttg cgcgtttatc acggtcccga
      661 ggaagccgct tttgacgctt cgccggcgac gatcccgttg cacgaccagg tcaagctgac
      721 gctgggcgag gtgctgccgc tgattctgga cgcggtgaag agccgccgct cgtgggtcga
      781 tgacttcgcc gacgacgaga tctcgatttc gaccgacctg tacgaggtgt tgctggcgta
      841 tcagcatttc cgccggccga gcgcctaagc gatttgggcg caagcactta acagctatcg
      901 cggcccggct cctgccgggc cgtttcgctg cgccgtggta ggttccgacg gccgcggcgg
      961 aaccagaggc gcccatcacc tcaactcgca ccgcaggtcc cgcccaggcg gtcgggacct
     1021 acaattaagt gccgacaata tcaggtggag cagccgcgtg aacgacgact ctttcttccg
     1081 ggcctcggac gaagacattt cgagctacga tctcgcgacg cagaagcacg accgccgctc
     1141 gaatatcgac gacctgatcc agacgatcaa ggaaacggcc gacaagctgg cggctgacgg
     1201 ctcgacgcgg ggcgatctga agatcctcag ccgcacgctt cgcgagctgc gatacgcctt
     1261 caaggtcttt gccccctatc gccgcaagcg aaaggtgacg gtctttggct cggcccgcac
     1321 gcccccggag catcccgcct atcagcaggc ggtcgccttc ggccgggcga tggccgaagc
     1381 cggctggctg gtcgttacgg gggcggcggc gggcatcatg gaggccggcc acgtcggcgc
     1441 tggccgtgat tactcgatgg gcctgaacat catgctcccc ttcgagcaat cggcaaatcc
     1501 ggtcatcgcg ggcgacatca agctcgtcca catgaagtac ttcttcacac gaaagctgat
     1561 gttcgtgaag gagtgcgatg cggtcgtctg ttcgcccggc ggcttcggca cgctggatga
     1621 ggcgatggag gtcattacgc tgttgcagac cggaaagcgg gacatgattc ccgttgtgct
     1681 tctcgatccg cccggcagcg gctattggca agggctgaat gatttcatcc acaaccagct
     1741 tcttcgcaac cgcctgattt cgccggagga cttgcacctc ttcaagttga cggatgaccc
     1801 tgaagcggcc aagggcgaag tgctgcagtt cttccgtgtt tatcacagca tgcggtacgt
     1861 caagaatgag ctggtaattc gcgtgcagcg gccgcttgat gcgccgtttc tggaggcgat
     1921 caacaccggg ttctcagacc tcctggtaac cggccgcttc gagcagcgcg aggccctgcc
     1981 gctggagcgc gacgaaacgg acctggccta cttgccgcgg ctggtgtttc aattcaaccg
     2041 gcggaacttc ggacggctgc gactgctcat tgatgcgatc aatcggggga gcgtgacctg
     2101 agcagcgatg cgggagtcgt tcagcggata gccgcaggca ccccgccgga agtttctggc
     2161 ctatcagcgg gatggc
//
LOCUS       DASUGB010000086         8085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_705014, whole genome shotgun sequence.
ACCESSION   DASUGB010000086 DASUGB010000000
VERSION     DASUGB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705014"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1081)
                     /locus_tag="VFV87_01630"
     CDS             complement(155..1081)
                     /locus_tag="VFV87_01630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5102480.1"
                     /translation="MDFNIVGLPFTVALAIVAVLGYFVGRIQRSKNQEPEDESTRREL
                     KRAKAIIHDLETIAQNIRRDLAQHHTNLEQFKTRIGKLSRNQESTNWQVLCDEAERLL
                     KPTQDLARQLAQAYDGIRQQGSRLMTFTAQRCDPLTGLCNRQALDESLENYLSMYSRY
                     GLTFSLAVFDLDGFQQFNADRGHREGDRILGQIAQLLEQNARETDIVARSGGEEFIVL
                     LPSSDLTAASLFAERIRHTIDELSDVTVSSGVAVVAADDDAKTLLTRADTALYAAKAA
                     GRNRVFSHTGQTVLPCQTEAADTMTEAEAAVT"
     gene            complement(1239..1385)
                     /locus_tag="VFV87_01635"
     CDS             complement(1239..1385)
                     /locus_tag="VFV87_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102481.1"
                     /translation="MANEFWNYVNLVTRSASQLDRTHWVVISILVLVIGLICMRGFGS
                     RNYY"
     gene            complement(1439..1564)
                     /locus_tag="VFV87_01640"
     CDS             complement(1439..1564)
                     /locus_tag="VFV87_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102482.1"
                     /translation="MLTRPVLRIGRLPVGPRGQALRIPSIVTVVGFWTGMVSAVS"
     gene            1635..1708
                     /locus_tag="VFV87_01645"
     tRNA            1635..1708
                     /locus_tag="VFV87_01645"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1669..1671,aa:Asp,seq:gtc)
     gene            complement(1988..3739)
                     /gene="polX"
                     /locus_tag="VFV87_01650"
     CDS             complement(1988..3739)
                     /gene="polX"
                     /locus_tag="VFV87_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX5102483.1"
                     /translation="MTNAHIADAFVLIGDILDFQGANPFRVRAYRNSARTIRDYPEPL
                     SAIVEADKSKLTDIDGIGDDLAAKIVTLVTTGKIPQLEELKSQVPESVLALLRIPGVG
                     PKKAAALHKLLGVKSLADLKAACESGQVAKIKGFGEKTQQTILQGIGFAETTTQRMLW
                     CEADQIVTELRERFRGCPGVKQLEFAGSYRRGKETVGDLDILAVASRPDEAMDCLAAF
                     PELDQILGRGDTKMSIRTHSGLQIDLRVVPAESFGAALQYFTGSKEHNIILRGRAKDK
                     GLKINEYGVFRVKADQETKIAGATEKEVYDTLDLPVFSPEIREGRREFEWADAGELPP
                     LITEADIRGDLHMHTTASDGVASIEEMVSAARERGLSYIAITDHSQRVSMANGLTPQR
                     ALAQWKEIDAIREKLPKGFTLLKGIECDILEKGGMDLPDDVLAQADWVIGSVHYGQKQ
                     SRQQITDRILGALENPHVDVIAHPTGRLINRRDAYQVDLDAVMAAAKKHGKLLELNSN
                     PLRLDLNEVYCAAARQHGIPIVINTDAHAPDQLGLLRYGIIQARRAGLTKSDVANTRT
                     WPNMKKLLGKESSSLTC"
     gene            complement(3821..4216)
                     /locus_tag="VFV87_01655"
     CDS             complement(3821..4216)
                     /locus_tag="VFV87_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5102484.1"
                     /translation="MTVRNYRDLLVWQKSMDLVMSVYGLTSRFPKQETYGLSSQMQRA
                     AVSIPANIAEGHDRDSTKEYLHSLAIAVGSLAELETLLAIARRLTYLDAKRYGEHEVA
                     CQTLGRMLRNLQRALRARVRRKRKEGDDS"
     gene            complement(4292..5326)
                     /locus_tag="VFV87_01660"
     CDS             complement(4292..5326)
                     /locus_tag="VFV87_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5102485.1"
                     /translation="MSLWTERLRITEMPANLTPQYKKAEEEYRRAQTPEDEVRCLELM
                     LREIPKHKGTDKLQAELKAKISKAKAEVEHAKKAKKGHHAGPHIPRQGAGRVAIIGGP
                     NAGKSSLLAKLTRATPEIAPYPFTTRDPQPGMMPWEDVQVQLIDTPPITADVLDPFVL
                     GLIRGSDLALLLFDAGSDEGLDALQAAVEHLKQTKTRLAKESYLDEDDVGLSFTQTFL
                     VHNKMDLPGAEDRIALLKEFCPLDFREFRISAEHGIGLEDLRNAIYGALGVVRVYTKL
                     PTKKEADYDKPFTLKRGGTLLDVAELVHRDMAASFKYARVWGTAVLAPGTQMKGDYVV
                     HDKDVVEIHA"
     gene            5380..6135
                     /locus_tag="VFV87_01665"
     CDS             5380..6135
                     /locus_tag="VFV87_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5102486.1"
                     /translation="MATPKVALVTGSGKKRVGSYVAAALAQRGYSLAVHFRTSAEEAR
                     ETIHALRNLGAPTEGFQADLTDESAVQKLMQLVRSKFGQIGVLVNCAAAWQPKKLEEV
                     TAADVRHYFETNTLSTFLTAQHAGLAMVQQPEGGSIVNIGDWAIVRPYLNYAAYFPSK
                     GAIPTLTRTLAVELGTRNPNVRVNCVLPGPVMLPPDLPASERQAAIDATLVKREGRPE
                     NVAQAVLHFIENDFITGACLPVDGGRSVYAGPH"
     gene            6451..>8085
                     /locus_tag="VFV87_01670"
     CDS             6451..>8085
                     /locus_tag="VFV87_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102487.1"
                     /translation="MAPILVAHCLDCHQPNKRSGELDLSTRAGLLAGGEQGPAITPGK
                     PAASLLVERISAGEMPPPDAKESHPLDKSQIEALRAWIAAGATWPNDRQLGIHERPID
                     LETAREFWSFQPVRRPPLPLPLGEGQGEGASVRNPQSAIRTPIDPFILQKLAAAELSL
                     SPPAAKNDLLRRLSLDLRGLPPSLDELEQFLSDHAPDAYERQVDRMLADPAHGERWAR
                     HWLDLVRYADSNGYERDGPKPSVWRYRDYVIAALNSDKPYDRFVHEQIAGDELADAND
                     ETLIATGFHALGAWQDEVDPLEAAQYRADELDDMIRTTSQTFLGLTLGCARCHNHKFD
                     PLTMVDYYSLAAVLAPLKRPNKGRLDRDVPLGTPEQIAALDARNEELGKLDGQIIQII
                     TQKPADADKQMAEIRRRQGELRAKTPDMPAAYRCFEDSAEAPPTYLLLSGRASNPGPQ
                     MQPRVPAVLVAQGRGLADFGQRGASPAAKSTGRRLAFGRWLVSEENPLTARVIVNRVW
                     QHHFGRGLVGTSSNFGALGERPTHPELLDFLAVRFME"
BASE COUNT         1422 a         2553 c         2601 g         1509 t
ORIGIN      
        1 aaaatcagga cgagacgatt caggcgatgc gcagcgagct cgaagcgctc cgcaagttga
       61 ttacgcccaa gaagtaggtt ccgactgcca cggcggtcct ggacgtgcca accggcgccg
      121 accgccgcac gggtcccgcc caggcggtcg ggacctacgt caccgccgct tcggcttccg
      181 tcatcgtatc cgccgcttcc gtctggcacg gcagcactgt ctggcccgtg tgcgagaaga
      241 cgcggttgcg tcccgccgct ttcgcggcat acagcgccgt gtcggcccgc gtgaggagtg
      301 tcttcgcgtc gtcgtctgcg gctacgaccg ccacgccgct gctgaccgtc acgtcggaca
      361 attcgtcgat cgtgtgccgg atgcgctccg caaacaagct tgccgccgtc aagtcggaac
      421 tgggcaacag cacgatgaac tcctcgccgc ccgagcgggc gacgatgtcg gtttcgcggg
      481 cgttttgctc caggagctgg gcgatttggc ccaggatgcg gtcgccttcg cggtgtcccc
      541 gatccgcgtt gaactgctga aagccgtcca gatcgaacac ggcgagcgag aacgtcagcc
      601 cgtagcggct atacatcgag aggtagttct cgagcgattc gtccagcgcc tgccggttgc
      661 acaggccggt cagcggatcg caccgctggg cggtgaaggt catcaagcgg cttccctgct
      721 gccggatgcc gtcataggct tgcgcgagct gccgggccag gtcctgcgtc ggcttgagca
      781 ggcgctcggc ctcgtcgcag aggacctgcc aattggtcga ctcctggttg cgggacagct
      841 tgccgatccg cgtcttgaac tgctccagat tcgtgtggtg ctgggccagg tcgcggcgga
      901 tgttttgggc gatcgtttcc agatcgtgga tgatcgcctt ggcccgcttc agttcgcggc
      961 gggtcgattc gtcctcgggc tcctggtttt tcgaccgctg gatgcggccg acgaaataac
     1021 cgagcacggc gacaatcgcc agggcaaccg tgaagggcaa gccgacaatg ttgaaatcca
     1081 tggggagcac tccgaggggc attccggcag cggaaccagc ggcgcgccgc tgcgaacctt
     1141 tctgccggac gagcgtcgta ctttgcgaat tagtagggtg ggctttgccc accaccgaca
     1201 tacaacgaat ctgaggtggg cacagcccac cctacggatc aatagtagtt ccgcgatccg
     1261 aacccgcgca tgcagatcag gccgatcacc aggaccagga tcgaaatgac gacccagtgc
     1321 gtgcgatcaa gctgcgacgc gctgcgcgtc acgagattga cgtagttcca gaattcattg
     1381 gccatacgaa cacctagctt actccgaggc aggaaacgga cgatccactg tcaaaaccct
     1441 agctcaccgc cgataccatg ccggtccaga accctacgac cgtcacgatg gagggaatcc
     1501 gcaaggcctg cccccgcggc cccaccggca ggcggccgat cctgaggact ggccgggtta
     1561 gcacgaaagc cggcctccga acgcccttgc gacccctgcc ggcgacgtta gaattcagga
     1621 ttgtgagatt tgttggggcc gtagctcaat tggttagagt accggactgt cgatccggtg
     1681 gttgcgggtt cgagccccgt cggcctcgct cgtaaataac tgccagtctt tgacttgccg
     1741 ccgatgcgcc ggcggctaaa cgtttgctgc ggcgggcgtg agaatccatc gctcgccgct
     1801 ctcgcgcgac cgccattgag caggctcttt gcgaaatgtc gaaaacgctt caatgcactg
     1861 cggaatcgac gatctgcgaa gagaagtaag cgggtaatcc gggcgctgga gctgtttcaa
     1921 gaactgcgga gcgccgagtg cagggactat cgaaccagcg ggttgcacca gggcgaattg
     1981 cccgcgacta gcaagtcagg gaagacgatt ctttaccgag cagcttcttc atgttcggcc
     2041 aggtgcgggt gttggcgacg tcggacttcg tgaggccggc gcggcgggct tggataatgc
     2101 cgtaacgcag caggccgagc tggtcggggg cgtgggcgtc ggtgttgatc acaatcggga
     2161 tgccgtgctg cctggcggcg gcgcaataga cctcgttcag gtccagccgc agcggattgg
     2221 aattcagctc cagcagcttg ccgtgctttt ttgcagcggc catgacggcg tcgaggtcaa
     2281 cctggtaggc gtcgcggcga ttgatcaaac ggccggtggg atgggcgatt acatccacgt
     2341 gtggattctc gagagcgccg agaatgcggt cggtgatctg ctggcgagac tgcttttggc
     2401 cataatgcac gctgccaatc acccagtcgg cctgggcgag cacgtcgtcg ggaaggtcca
     2461 tgcccccttt ttcgagaata tcgcactcga tccctttgag aagcgtgaat cccttgggga
     2521 gtttttcgcg aatcgcgtca atttctttcc actgggccaa tgcccgctgg ggagtgagcc
     2581 cgttggccat gctgacgcgc tgcgagtgat cggtgatggc gatgtacgac aatccgcgct
     2641 cgcgagccgc ggagaccatc tcctcgatcg acgcgacgcc gtcggaagcc gtcgtgtgca
     2701 tgtgcagatc gccgcggata tcggcttcgg tgatcagcgg gggcaactcg cccgcgtcgg
     2761 cccattcgaa ctcgcgtcgg ccctcgcgga tctccggcga aaagaccggc agatcgagcg
     2821 tgtcatagac ttccttctcg gtcgcgccgg cgatcttggt ctcctggtct gctttgacgc
     2881 ggaacacgcc gtactcgttg atcttcaggc ctttgtcttt ggcccggccg cggagaatga
     2941 tgttgtgctc cttggagccg gtgaagtatt gcagcgcagc tccgaatgac tcggccggca
     3001 cgactcgcag atcgatctgc aagccgctgt gggtgcggat cgacatcttc gtatcgccac
     3061 ggccgaggat ttgatcgagc tcgggaaagg ccgccaggca atccatggct tcatcgggcc
     3121 ggctggcgac ggcgagaata tcgagatcgc cgaccgtctc tttgccgcgg cgatagctgc
     3181 cggcgaattc gagctgcttc acgcccgggc agccgcgaaa ccgctcccgc agctcggtta
     3241 cgatctggtc ggcttcgcac cagagcattc gctgcgtcgt cgtctcggcg aagccgatgc
     3301 cttgcaggat tgtctgctgg gtcttttcac cgaagccttt gattttggcg acctggccgg
     3361 attcgcaggc ggctttcagg tcggcgagcg acttgacacc gagcagcttg tgcagggcgg
     3421 ccgctttttt cggcccgacg ccgggaatcc gcaagagggc cagcacgctc tcgggaacct
     3481 gcgatttcag ctcctccagc tgcggaatct tgccggtggt gaccagcgtg acgatcttcg
     3541 ccgccaggtc gtcgccaatg ccgtcgatat cggtgagctt gctcttgtcc gcttcgacga
     3601 tcgccgagag cggctcggga tagtcgcgta tcgtccgtgc gctgttgcga tacgctcgca
     3661 cacggaacgg attggccccc tggaaatcga ggatgtcgcc gatcaggaca aaggcgtccg
     3721 cgatgtgagc gttggtcatg gcgcgagggg acggggaaca ggggacgggg gccgggggcg
     3781 ccccccgaca gcacaacgtc gattttacaa gggacagcga ttaggagtcg tcgccttcct
     3841 tgcgcttgcg gcggacgcgg gctcgtagcg cccgctgcag gttgcggagc atgcggccga
     3901 gagtctggca ggcgacctcg tgctcgccgt accgcttggc gtcgagatac gtgagtcgcc
     3961 gagcaatcgc cagaagcgtt tccagctctg ctaaagagcc tacggcgatc gcgagtgaat
     4021 gaaggtattc cttcgtcgag tcgcgatcat gaccctcagc aatgttggcc ggaatggaaa
     4081 ccgcggcccg ctgcatctgg ctcgatagcc catacgtttc ctgtttggga aatcggctcg
     4141 ttagcccata gacggacatc acaaggtcca tcgacttttg ccacaccaac aggtcgcgat
     4201 agttgcgcac ggtcattcga aagtctccag gttgatagaa cacaagcgaa atgccccact
     4261 gcccctgtcc cccgttccct gtcccctgcc ctcacgcgtg aatctccacc acgtccttat
     4321 cgtgtacgac atagtccccc ttcatctgcg tgccgggggc gaggacggcg gtgccccaga
     4381 cccgggcgta tttgaaactg gcggccatgt cgcggtggac cagttcggcc acgtcgagca
     4441 gcgtgccgcc gcgcttgagc gtaaaaggct tgtcgtagtc ggcttctttc ttggtgggca
     4501 gcttggtata gacgcggacg acgcccaagg ccccgtagat cgcgttgcgg aggtcttcca
     4561 ggccgatgcc atgctcggcg gagatgcgga attcgcggaa gtcgagcggg cagaactcct
     4621 tcagcaaggc gatccggtct tcggcgccgg gcagatccat cttattgtgg accaggaacg
     4681 tctgcgtgaa ggagaggccg acgtcgtctt cgtcaagata agactccttg gccaaccgcg
     4741 ttttggtttg cttcaagtgt tcgacggcgg cttgcagggc gtcgagcccc tcgtccgagc
     4801 cggcgtcgaa cagcagcagg gccaggtcgc tgccgcgaat cagccccagg acgaagggat
     4861 cgagcacatc ggccgtgatc ggcggcgtgt cgatgagctg cacctgcacg tcttcccagg
     4921 gcatcatccc cggctgcggg tcgcgggtgg tgaagggata aggagcaatt tccggcgtgg
     4981 cccgcgtcag cttggccagg agcgagcttt tgccggcgtt gggtccgccg atgatcgcga
     5041 cgcggccggc cccctgccgc gggatgtgcg gcccggcgtg atgccccttc ttggccttct
     5101 ttgcgtgctc aacttccgcc ttggccttgc tgatcttggc cttcagctcg gcctgcagct
     5161 tgtcggtgcc tttgtgcttg ggaatctccc gcagcatcag ctccaaacag cggacttcgt
     5221 cctccggcgt ctgcgcgcgg cggtattcct cttcggcttt tttgtactgg ggggtgaggt
     5281 tggcaggcat ctcggtgatc cgcagccgtt ctgtccacag gctcatgata tgccaggctc
     5341 tgcgatgccg ctgccgggcg gcatgttacg atagtcggca tggcaactcc caaggtcgca
     5401 ttggtcaccg gcagcggcaa gaaacgcgtc ggctcgtacg tcgctgcggc gctggcgcag
     5461 cggggctatt cgcttgccgt gcattttcgc acctcggcgg aagaggcccg cgagacgatc
     5521 cacgcgctgc ggaatctagg agcgccgacg gaaggctttc aggccgatct gaccgatgaa
     5581 tcggcagtgc aaaaactgat gcagctcgta cgctccaaat tcgggcaaat tggcgtgctg
     5641 gtcaactgtg ccgccgcctg gcagccgaag aagctggaag aggtgacggc tgcggacgtg
     5701 cggcactact tcgaaacgaa cacgctcagc acgttcctca cggcccagca cgccggcctg
     5761 gcgatggtcc agcagccgga ggggggctcg attgtcaaca ttggcgactg ggcgatcgtg
     5821 cggccgtatc tcaactacgc cgcctatttt ccgtcgaaag gcgcgattcc gacgctgacc
     5881 cgcacgctcg cggtcgagct tggcacgcgg aacccgaacg tgcgcgtgaa ctgcgtgctg
     5941 cccggtccgg tcatgctgcc gcccgatttg cccgccagcg agcgtcaggc cgcgatcgac
     6001 gccacgctcg tcaaacgcga aggccgcccg gagaacgtcg cccaggccgt actgcacttc
     6061 atcgagaacg atttcatcac cggcgcctgc ctgcccgtcg atggcgggcg aagcgtttat
     6121 gccgggccac actaacccgc agcgtcagcg agggactccc gtcgcgacgg cattgggaac
     6181 agaagttcgc aaagggagca aagaagcaag ggaaatgtgt gaacccatct cgccttccaa
     6241 cacctctccc tttgcgatct tcgcgtcctt ctgttcaaaa ctccctcgcc cttcgccttg
     6301 catcccatcc ggccctgttt gacaatgaat cgctgtcaaa cacgcgcggc gagagcgagc
     6361 atgcggcaaa ggttcttgat actgtggact tgccttctgt cgccggcaac aggagcgcag
     6421 gcagcagagc cgccggattt tgagcgcgac gtggcgccga ttctggtggc gcactgcctc
     6481 gattgccatc agccgaataa acgcagcggc gagctggatc tttcgacgcg cgcggggctg
     6541 ctggcggggg gcgagcaggg gccggcaatc acgcccggca agccggcagc gagcctgctg
     6601 gtagagcgga tcagtgccgg cgaaatgcct ccgccggacg ccaaggaatc gcacccgctg
     6661 gataaatcgc aaatcgaggc cctgcgggcg tggatcgccg ccggagcgac gtggccgaac
     6721 gatcgccagc tcggcatcca cgagcgcccg atcgacctgg aaacggcccg cgaattctgg
     6781 tccttccagc cagtgcggcg tccgcctctc cctctccccc tgggagaggg ccagggcgag
     6841 ggtgcatcag tccgcaatcc gcaatccgca atccgcactc ccatcgaccc cttcatcctg
     6901 cagaagctcg cggcggccga actttcactc tctccccccg ccgccaagaa cgacctgctc
     6961 cgccgactct cgctcgacct ccgcggcttg ccgccgtcgc tcgatgagct ggagcaattc
     7021 ttaagcgacc acgcgcctga tgcctacgag cgccaggtcg atcggatgct cgccgatccg
     7081 gcgcacggcg agcgctgggc ccggcactgg ctcgacctgg tgcgctatgc cgacagcaac
     7141 ggctacgagc gggatggccc caagccgagc gtgtggcggt accgcgacta cgtcatcgcc
     7201 gccctcaaca gcgacaagcc gtacgaccgc ttcgtgcacg agcagatcgc cggcgacgag
     7261 ctggcggatg cgaacgacga aacgttgatt gcgaccggat tccatgcgct cggcgcctgg
     7321 caggacgagg tcgatccgct cgaagcggcg cagtaccggg ccgacgaatt ggacgacatg
     7381 atccggacca cgtcgcagac gtttctgggc ctgacgctcg gctgcgcccg ctgccacaac
     7441 cataaattcg atccgctgac gatggtcgat tactacagcc tggccgcggt ccttgcgccg
     7501 ctgaagcggc ccaacaaggg ccgcctggat cgcgatgttc cgctcggcac gccggagcag
     7561 atcgcggccc tggatgcccg caacgaagag ctgggcaagt tggatggcca gatcatccag
     7621 attatcaccc agaagccggc cgacgccgac aagcagatgg cggagattcg ccgccggcag
     7681 ggcgagctgc gggccaagac gccggacatg ccagcggcct atcgctgttt cgaggacagc
     7741 gccgaggcgc cgccgaccta cctgctgctc tcgggacggg cgagcaatcc ggggccccaa
     7801 atgcagccgc gggtgcccgc ggtgctggtc gcccaggggc gcggcctggc ggactttggc
     7861 cagcgcggtg cgagccccgc ggcgaaatcg acgggaaggc gtttggcgtt tggccgctgg
     7921 cttgtcagcg aagaaaatcc cttgacggcc cgcgtcatcg tcaaccgcgt ctggcagcac
     7981 cacttcggcc gcggactcgt gggcacgtcg tccaacttcg gcgcgctcgg cgaacggccg
     8041 acgcaccccg aactgctcga cttcctggcc gtccgcttca tggaa
//
LOCUS       DASUGB010000087         5275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_566359, whole genome shotgun sequence.
ACCESSION   DASUGB010000087 DASUGB010000000
VERSION     DASUGB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566359"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV87_01675"
     CDS             complement(<1..183)
                     /locus_tag="VFV87_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102488.1"
                     /translation="MDSALTLLAQAAAPDGDGGAGALLLGGGCMLVWMVVVLAALVLW
                     IWALVDAIRNPALDNNS"
     gene            310..2076
                     /locus_tag="VFV87_01680"
     CDS             310..2076
                     /locus_tag="VFV87_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5102489.1"
                     /translation="MPCIARTFVARAALACAALALPSGCGGTPALDSQPSPQSAQEHA
                     PWFADVTSESGIDFTHDPGPIDGRYFMPQINGSGAALFDCNNDGRLDLYLLQFGGPDS
                     NSRNALYLQQPSGKFQDISPGSGLDINGHNHGVAVADVNNDGWQDVLVTQYLGARLFL
                     NQTDNTFRDVTGELELTNPYWGASAGFADFDRDGWLDLAIVNYIEFDESRYCTARGGK
                     RDYCLPSIFEAESTRLWRNRGADSAGPWQGYEDVTAASGLAAKPGPGMGVLCADLSGD
                     GWPDLFVANDLEPNHLWINQRDGTFQEEAVTRGLAYNARGLAQSDMGAALGDVDGDLL
                     PDVFVTHFTDEHHGLWKQRAAGLFQEETIAAGLSNSAWHGTAWGTTLTDFDQDGWLDV
                     ALVNGFVQRRDAPSGTFWQDYMDRNQLFASEGQGRFRDVSLSNPALCGGPNVGRGLCA
                     GDLDGDGAIDLVVTQIGGPVRILRNVAPNRGHWLLVRAVDPALQRDAIGAEIVLAAGG
                     RRWLGLVQPGQGFQCAHDPRVHFGLGAADRIDSIEVRWPDGSRERFAGGPADRALELR
                     RGEGSPSAENHGPAHGSPEPVE"
     gene            2051..3328
                     /locus_tag="VFV87_01685"
     CDS             2051..3328
                     /locus_tag="VFV87_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5102490.1"
                     /translation="MAAQSQSSNAERASSSARPAAAKRTRRRFLALAFGGFSLLLLVG
                     GAALYVYRHTPPGEMPALNLDDADPDLVELVREAQAQIEHAPRSAEAWGRLAMILHAN
                     GFSSAAQVCYRYAGQLAPDDPKWPYLQGYLFHHGPGGPPTSLPYFERAARLSPDNWLL
                     QLRLAEAELETGRLAAAAERYRGLLATNYDEGPARLGLAKVALTWGRLDEALEQLRSI
                     EANPAVQKEAATIRASILYGQGDADAAAQERQRAERLPEDPLLPHDPVAEVAAFEVGV
                     PMRLQVADRLLAAREIPEMLHLIQETTEKYPHSVEAWAALGNARGMLNDPVGAERALL
                     NCVELAPHSAEHHARLAQVQVWLRQYSRAEESLRRALELDPQSATAHFALGECRQQQG
                     DTAGAIKAYREALRLMPDHPLAKKRLDELAARP"
     gene            3433..4773
                     /locus_tag="VFV87_01690"
     CDS             3433..4773
                     /locus_tag="VFV87_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102491.1"
                     /translation="MRFVATFLAALLALASIAGAETVNWPQYRGAAADGMAEGATLPE
                     KWSPTENVVWKAAIPGWGWSSPVIWEGKIFLTSAVGEHELQRPVVGGYPGGRVQAGDV
                     HRWMTYCLDFDSGNILWEREAHQGIPPQERHPRNSFASETQVTDGERIYSYFANIGLF
                     CYDLDGNKLWEKRWPSYPMRGGWGTGTSPVLHGERIFIVNDNEQESFIVALDKRTGEQ
                     IWRQEREEKSNWSTPYIWQHDLRTELVTIGTGKIRSQDLDGNLLWELTGTSGLVSLTP
                     VARHGLLYVGAGYHYGPLYAVRPGAKGDISLTGDETSNEFIAWSQPRGASIHPCFLVS
                     DERLFVLFDAGLLACSNALTGETIFTRQRLNTGGGRFYASPWAYNGKIFLLNEDGTTW
                     VVEDGPEFNVLHKNVLEDNAWATPAISRGSLFLRTYTSLYRLQDQDAAAKGDAK"
     gene            4770..>5275
                     /locus_tag="VFV87_01695"
     CDS             4770..>5275
                     /locus_tag="VFV87_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102492.1"
                     /translation="MTRCIGLVSIVVLVSVAAAAETSWTKLLEDRNKRGTLEGTRQVV
                     RGDTGKWSEEPRLQHAPPLARKPAKLPLDEWGDRLMDEKFELTAADENWLLLRTIQLN
                     DNDRIWVQSVERKGNQLTVVACQAVWQGRYPKNFTQHFVLGVNLGKLEPGKYQTKWIL
                     QPLQFTKF"
BASE COUNT          927 a         1828 c         1688 g          832 t
ORIGIN      
        1 cgagttgttg tccagcgccg gattgcgaat cgcatcgacc agggcccaaa tccagagcac
       61 cagcgccgcg agaaccacca ccatccagac cagcatgcac cctccgccca ggagcagggc
      121 gccggcgccg ccgtcgccgt cgggcgccgc agcttgagcg agcaatgtga gagcgctatc
      181 catggtgcaa ctccttttcg gcaaaaccag atgaatggat cacgggcatt tcttgacaga
      241 ttcgttcgcg ccgtcctatc gtagaggcgg attcgtcttg gggaaaatct tctgggggaa
      301 ggcggaacca tgccgtgcat cgcgcggacg ttcgtcgctc gtgctgcgct cgcctgtgcc
      361 gcgctcgcgc tgccctcggg atgtggcggc acgcccgcgc tcgattcgca gccttcgccc
      421 cagtccgcgc aggagcatgc gccctggttc gccgatgtaa cgtctgaatc gggcatcgac
      481 ttcacccacg accccggccc gatcgacggc cgctacttca tgccccaaat caacggctcg
      541 ggggcggcgc tcttcgattg caacaacgac ggccgcctcg atctgtacct cctgcagttc
      601 ggcggccccg attccaactc gcggaacgca ctctacctgc aacagcccag cggcaagttc
      661 caggacatca gcccgggctc cggcctggac atcaacggcc acaaccacgg cgtcgccgtg
      721 gccgacgtga acaacgacgg ctggcaggac gtgctcgtca cgcaatacct cggggcccgg
      781 ctgtttctca atcagacgga caatacgttc cgcgacgtga ccggcgaact cgagctgacc
      841 aatccttact ggggcgcttc ggccggcttc gccgacttcg accgcgacgg ctggctggac
      901 ctggcgatcg tcaactacat cgagttcgat gaatcgcgct attgcaccgc gcgcggcggc
      961 aagcgcgact attgcctgcc gagcatcttc gaggcggaaa gcacgcggct ctggcgcaac
     1021 cgcggcgcgg actccgcggg cccgtggcaa ggctacgagg acgtcaccgc cgcatccggc
     1081 ctggcggcca aacccggtcc cggcatgggc gtgctatgtg ccgacctgag cggcgacggc
     1141 tggcccgatc tcttcgtcgc caacgacctg gagcccaacc acctgtggat caaccagcgc
     1201 gacggcacgt ttcaggaaga agcggtcacg cgcggcctgg cctacaacgc ccgtggcctg
     1261 gcccaatccg atatgggagc ggcactgggc gacgtcgatg gcgatctgct ccccgacgtc
     1321 ttcgtcaccc atttcaccga cgagcatcac ggcctctgga agcaacgcgc cgcgggcctg
     1381 tttcaggaag agaccatcgc cgccggactc agcaacagcg cctggcacgg caccgcctgg
     1441 ggaacgacgc tgaccgattt tgaccaggat ggctggctcg acgtggcgct ggtcaacggc
     1501 ttcgtgcagc gccgcgatgc gcccagcggc acattctggc aagactacat ggatcgcaac
     1561 cagctcttcg cgagcgaagg ccagggccgc tttcgcgatg tttcgctttc caatccggcg
     1621 ctgtgcggcg ggccgaacgt cggccgcggc ctgtgcgccg gcgatctgga cggcgacggg
     1681 gcgatcgacc tcgtcgtcac gcagatcggc gggcccgtgc ggatcctgcg gaacgttgca
     1741 cccaaccgcg gccactggct gctggtgcgg gccgtcgatc ccgcgctaca gcgcgacgcc
     1801 atcggcgccg aaatcgtgct cgccgccggc ggccgccgct ggctgggcct cgtccagccg
     1861 ggacagggct ttcagtgcgc gcacgatccg cgcgtccatt ttggcctggg cgctgcggac
     1921 cggatcgact cgatcgaagt ccgctggccc gacggcagcc gcgagcgttt cgccggcggc
     1981 ccggccgatc gcgccctgga attgcgtcgc ggcgagggat cgcccagcgc agaaaaccac
     2041 ggacctgctc atggcagccc agagccagtc gagtaacgcc gagcgcgcct cgagttccgc
     2101 gcggccggcc gccgcgaagc ggacgcgccg ccgcttcctc gcgctggcgt tcggcggatt
     2161 ttcgctcctc ctgctggtcg gcggcgcggc gctctacgtc tatcgccaca caccgccggg
     2221 cgaaatgccc gccctcaacc tcgacgacgc cgaccccgac ctggtcgagc tcgtgcgcga
     2281 ggcccaagcc cagatcgaac acgcgccgcg ctccgccgag gcctggggcc ggctggccat
     2341 gattctgcac gccaacggct tcagctccgc cgcgcaggtc tgctatcgct atgccggcca
     2401 gctcgccccg gatgatccca agtggccgta tttgcaaggt tacttgtttc accacggtcc
     2461 gggcggcccg cccacgtcgc tcccctactt cgagcgcgcc gcccgcctca gcccggacaa
     2521 ttggctcctg cagttgcgtc tggccgaggc cgagttggaa accggccgtc tggccgctgc
     2581 cgccgagcgg taccgcgggc tcctcgcgac gaactacgac gaaggaccgg cccgcttggg
     2641 gctggcgaaa gtggcgctga cctgggggcg gctggatgaa gcgctcgaac aactgcggtc
     2701 gatcgaggcg aatcccgccg tgcaaaaaga agcggccacg atccgggcca gcattctgta
     2761 tggccagggc gatgccgacg cggcagcgca ggagcggcag cgcgcggagc gactgcctga
     2821 agacccgctg ttgccccacg atcccgtggc ggaagtggcg gccttcgaag tcggagtccc
     2881 catgcgcttg caggtggctg atcgcttgtt ggcggcacga gaaattccag aaatgctcca
     2941 tttgattcag gaaaccacgg agaagtatcc gcattcggtc gaggcttggg cggcgctggg
     3001 aaatgcccgg gggatgctga acgatcccgt gggcgcagag cgggctctgc tcaactgcgt
     3061 cgagttggcg ccccatagcg ccgagcacca cgcccgactg gcccaggtcc aggtgtggct
     3121 gcgacaatat tcccgcgctg aggaatcgct gcggcgggcc ctcgaactcg acccccaaag
     3181 tgccaccgcc cactttgccc tgggcgaatg ccgccagcag cagggcgaca cggccggcgc
     3241 catcaaggcc tatcgcgaag ccctgcgcct catgccggac catcccctgg cgaaaaagcg
     3301 cctggacgag ctggccgcca ggccttaggc cggccccgtt gtgcgcgacc ggcgcggcgc
     3361 gtgtatttcg ccctgctgct ccgtagaatc acgctattca atcgcttcga actgcagtcg
     3421 aggttcccgg ccatgagatt cgtcgcaacc ttcctcgccg cgctgctcgc tctcgcttcg
     3481 atcgcgggag ccgaaaccgt caactggccc cagtaccgcg gcgccgcagc cgacgggatg
     3541 gccgaggggg ccacgctgcc ggagaagtgg agcccgaccg aaaacgtcgt ctggaaagcg
     3601 gccattcctg gctggggctg gtcgtcgccc gtgatttggg agggcaagat cttcctcacc
     3661 tccgccgtgg gcgagcatga gctgcagcgg ccggtggtcg gcggctatcc gggcggccgc
     3721 gtgcaggctg gtgacgtcca ccgctggatg acctactgcc tcgacttcga ttccggcaac
     3781 atcctctggg agcgcgaggc ccaccaggga atcccgccgc aggagcgcca cccgcgcaac
     3841 tcattcgcct ccgagaccca agtcaccgac ggcgagcgga tctattcgta cttcgccaac
     3901 atcggcctgt tctgctacga cctcgatggc aacaagctct gggagaagcg ctggccgagc
     3961 tatccgatgc gcggcggctg ggggacggga acttcgcccg tgctgcatgg cgagaggatt
     4021 ttcatcgtta acgacaacga gcaggagtcg ttcatcgtcg ccctcgacaa gcgcaccggc
     4081 gagcaaatct ggcggcagga gcgcgaggaa aagagcaact ggtccacgcc ctatatctgg
     4141 cagcacgacc tgcggaccga gctggtcacg atcggcaccg gcaagattcg ctcgcaggac
     4201 ctggacggca acctcctctg ggagctgacc ggcacctcgg ggctcgtcag cctgacgccc
     4261 gtcgccaggc acggcctgct ctacgtcggc gccggctatc attacggccc gctctacgcc
     4321 gtccgccccg gggccaaggg cgatatcagc cttaccggcg acgaaaccag caatgagttc
     4381 atcgcctggt cgcagccgcg aggcgcgagc atccacccgt gcttcctcgt ttcagacgag
     4441 cggctgttcg tgctgttcga tgcggggctt ttggcctgct cgaacgccct gaccggcgaa
     4501 acgatcttca cgcgccagcg gctgaacacc gggggcggcc ggttctatgc ctcgccgtgg
     4561 gcctacaacg gcaagatctt cctgttgaac gaagacggca cgacctgggt cgtcgaagac
     4621 ggccctgagt tcaacgtgct gcacaagaac gtcctggaag acaacgcctg ggccaccccc
     4681 gccatttccc gcggcagcct cttcctccgc acttacacca gcctctaccg gctgcaggac
     4741 caagatgccg cagccaaagg agacgcaaaa tgacccgctg catcggcctg gtgagcatcg
     4801 ttgtgctggt aagcgtcgcc gctgctgcgg aaacgtcgtg gacgaagctg ctcgaggacc
     4861 gcaacaagcg cggaacgctc gaagggacgc ggcaggtagt tcgcggcgac accggcaaat
     4921 ggagcgagga gccccgcctg cagcacgccc cgccgctcgc ccgcaaaccg gcgaagctgc
     4981 cgctcgacga gtggggcgac cggctgatgg acgaaaagtt cgagctgacc gcagccgacg
     5041 agaattggct gctgctgcgg acgatccagc tcaacgacaa cgaccgcatc tgggtccaga
     5101 gcgtcgagcg gaaagggaac cagctcacgg tcgtcgcctg ccaggcggtc tggcaggggc
     5161 gctatccgaa gaacttcacg cagcacttcg tcctcggcgt gaacctcggc aaactggagc
     5221 ccggcaaata ccagacaaag tggatcctcc agccgcttca gttcacgaaa ttcga
//
LOCUS       DASUGB010000088         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1956494, whole genome shotgun sequence.
ACCESSION   DASUGB010000088 DASUGB010000000
VERSION     DASUGB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956494"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFV87_01700"
     CDS             complement(<1..966)
                     /locus_tag="VFV87_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX5102493.1"
                     /translation="MGATPSLTTRRALLVASAAGVLTAAWPKRSFAQPPTGRRFAIAL
                     HGGAGKSPEKMTAEQIKAVEASLSKALAIGEQILKGGGTSLDAVEQVIRFLEDDPHFN
                     AGKGAVFNSAGGHELDASIMDGKTKACGAVAAVQTVKNPISLARLVMTKTRHVLLTSD
                     GAEKFADEMQVERVENKSFDTDKARREWEERKKADAARQANATSATTGRGFEEHYGTV
                     GCVALDTHGDLAAGTSTGGLTNKKYGRVGDSPIVGAGTYADNATCAVSGTGIGEEFIR
                     HAAAYDVSVRLAYLHQPLKEAVSDVLRKSLKPGDGGIIAVGSDGSI"
     gene            complement(1628..2068)
                     /locus_tag="VFV87_01705"
     CDS             complement(1628..2068)
                     /locus_tag="VFV87_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5102494.1"
                     /translation="MRTALGGLCLVLVAAVTQGQEERPAAKEVEVAVLAKTTKSWDGT
                     ALPAYPKGQPEVSIIRYTIPPKTRMALHKHTVINAGVLLKGKLTVVAEDDKVLEMKAG
                     DAIVELVDKWHYGVNSSDEPAEILVVYAGTEGQSNTLRPGEATR"
BASE COUNT          350 a          668 c          686 g          436 t
ORIGIN      
        1 gatcgagcca tccgatccga cggcgatgat cccgccgtct ccgggtttca ggctcttgcg
       61 cagcacgtcg gagacggcct ctttgagcgg ctgatggagg taggccaggc ggacggaaac
      121 gtcgtaagcg gcggcgtggc ggatgaactc ctcgccgatg ccggtgccgc tgacggcgca
      181 ggtggcgttg tcggcatacg tccccgcgcc gacgatcggc gaatcgccca cgcggccgta
      241 cttcttgttc gtcagcccgc cggtggaagt ccccgccgcc agatcgccgt gggtgtcgag
      301 agccacgcag ccgacggtgc cgtagtgctc ctcaaagccg cgaccagtgg tcgcggaagt
      361 cgcgttggcc tgcctggcgg cgtccgcttt cttgcgttct tcccattccc ggcgggcctt
      421 gtccgtgtca aacgacttgt tttcgacgcg ctcgacctgc atttcgtcgg caaacttctc
      481 cgcgccgtcg ctggtgagca ggacgtgccg cgtcttggtc atcacgaggc gggcgagcga
      541 gatcgggttc ttcaccgtct gaactgcggc caccgcgccg caggctttcg tcttgccgtc
      601 catgatcgag gcgtcgagtt cgtgtccgcc ggcgctgttg aataccgcgc ccttgccggc
      661 attgaagtgc ggatcgtcct ccaggaagcg gatcacctgc tcgacagcgt ccaggctcgt
      721 gccgccgcct ttcagaatct gttcgccgat cgccagcgcc ttggacagcg aagcctcgac
      781 ggccttgatc tgctcagccg tcatcttctc cggcgatttg cccgccccgc cgtgcagggc
      841 gatggcgaag cgccggcctg tgggcggctg cgcgaaactg cgtttcggcc aggcggcggt
      901 caagacaccg gcagcactag cgaccagcag cgcgcggcgc gtcgtaagcg agggagtggc
      961 tcccatcggt tcctccgagg gtgaagggcg caattggcgt gagcaatcgg ccccctagtt
     1021 taacggcgag ctgcaggcga tgccgcatcg caagccgcgg agaccaagaa acaagagaag
     1081 gggcgcaggt taacgggcag acctgcgccc cttcgggcgg gggtggcttg attgtcagag
     1141 tttgagagcc cctgctgcgc ccgggcgacc tgaatcgcga agacctctcc cttccccgag
     1201 attcaggcgc cgggcagcaa cgggcgatat cgaaggcact gggttgcggt ttccgcggga
     1261 gctttggctc tcacgcctgg gccggcaagc cgcgaagccg cacgtgcatc aacgatctac
     1321 tggcatggaa acatgggcgg gttaaaagtt cgccaagccg gggttcagct ttggctttgt
     1381 ggccaatctt gctgcggctg ggtgattgca taagccgtgc caaggcagca gccgggcaat
     1441 ggtctgggcc aatagtcagc gaattaagtg ttggtggcaa ttacacttgc gattacaagt
     1501 tcgccgtctc ttccagcgtt ccagtgcaac ttgagactga tagtgctgaa agcgagatgc
     1561 tcaagattcg ggcaatttgg tttctcgcca gctcccgcag caggcctcca gagccctact
     1621 ttcgctgcta tcgggtggcc tctcctgggc ggagggtgtt ggactgaccc tcggtgccgg
     1681 cgtagacgac gaggatttcg gcgggttcgt cggagctgtt gacgccgtag tgccacttat
     1741 cgacgagctc gacgatcgca tcgcccgctt tcatttcgag caccttgtcg tcttcggcca
     1801 ccaccgtcaa cttgcctttc aggaggacgc cggcgttgat caccgtgtgc ttgtgcagcg
     1861 ccatgcgggt cttcggcgga atcgtgtagc gaatgatcga cacctccggc tggcccttgg
     1921 gataagccgg caaggccgtg ccgtcccagc tcttggtcgt tttggccagc acggcgactt
     1981 ccacctcctt ggcagccggc cgttcttcct gcccctgcgt gaccgcagcc acgagcacca
     2041 gacacagacc gcccaatgca gttctcaagg tgtttctcct ctccagatag tctaggcgat
     2101 gtattccaat gtgcgtcgga tggcaacgca tcatgaagtt
//
LOCUS       DASUGB010000089         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_11228,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000089 DASUGB010000000
VERSION     DASUGB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11228"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFV87_01710"
     CDS             <1..1483
                     /locus_tag="VFV87_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5102495.1"
                     /translation="DLAISTVLTPQQRNYLSTAKESALSLLTLLNDVLDFSKIEAGRM
                     DLESIPFSVREVVEDAASTLSVNAAKKRLELIWHVEPDLPGLVIGDPGRLRQVIVNLI
                     GNAIKFTSEGEVYVRVEQDNSGAEPLVRFAVADTGIGIPPEKHATIFEAFKQSDSSTT
                     RRYGGTGLGLAITAELVTLMGGKIQIDSQSGAGSTFHFALPLSEAEPTGESPADQELQ
                     EPLYYCRVLLWSACKHHREALESLVEQLGAEVELLPADPFRAAKRRNAAGSDVLLVID
                     APAAAGLPFDPGALRARLGLAAEQVLILHMAGQSEVGGDCDPSGPVRFLTKPLRARDL
                     AAILAATEADEPVSRALAAGRKLHLLVADDSPVNQEVARGLLELHGHSVVTVGTGHEA
                     LEAWEREAFDVVLMDLEMPDMDGLEAAERIRRVEASTGRYTPIIALTAHVLKGVRERC
                     LAAGMDDCVTKPLLPDDLLPRIAALADLHSRSAAGRTAAVAAN"
     gene            complement(1511..1801)
                     /locus_tag="VFV87_01715"
     CDS             complement(1511..1801)
                     /locus_tag="VFV87_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102496.1"
                     /translation="MGNKLVLQRWVNYGMVAATVVWIVAIPMWGVLAYRAATTEHYQA
                     LYADRKDELFEIALGQALTSGLCCPTVPYAICMGMLALVTLALRPPGEKDNW"
BASE COUNT          388 a          657 c          664 g          376 t
ORIGIN      
        1 cgacctggcc atcagtacgg tgctgacgcc gcagcagcgg aactatctct caacggccaa
       61 agaatcggcc ctgtcgctgc ttacgctgct caacgacgtg ctcgattttt cgaagatcga
      121 ggcgggacgg atggatctgg aatcgatccc gttcagcgtt cgcgaggttg tcgaggacgc
      181 cgcgagcacg ctgtcggtca acgcggcaaa aaagcgcctg gagctgatct ggcacgtcga
      241 gccggacttg cccggactgg tgatcggcga tcctggccgc ttgcggcagg tgatcgtcaa
      301 cctgattggc aacgccatca aattcacgtc ggaaggcgag gtctatgtcc gcgtcgagca
      361 agacaattca ggcgctgagc cgctggtgcg atttgctgtg gcggatacgg gcatcggcat
      421 tccacccgag aagcacgcga cgatctttga ggctttcaag cagagcgaca gctccacgac
      481 gcggcgctac ggcggcaccg gcctgggcct ggcgatcacc gccgagctgg tcacactgat
      541 ggggggcaag attcagatcg acagtcagtc cggcgcggga agcacgttcc atttcgcgct
      601 gccgctgagc gaggcggagc cgaccggcga atcgcccgca gaccaggagc tgcaggaacc
      661 gctctactac tgccgcgtgc tgctgtggtc ggcctgtaag caccaccgcg aggcgctgga
      721 gtcgctggta gagcaactcg gggccgaggt cgagcttctg ccggccgatc cgttccgtgc
      781 cgccaagcga cgcaacgcag cggggagcga cgtgctattg gtcatcgacg ccccagctgc
      841 agccgggctg ccgttcgatc ccggggcgct gcgagctcgg ctgggcctgg cagccgagca
      901 ggtgctgatt cttcacatgg cgggccagtc ggaagtcggt ggggactgtg atccgtccgg
      961 gccggtccgg tttcttacca agcccttgcg ggcccgcgat ttagccgcaa ttcttgccgc
     1021 gaccgaagcc gacgagcccg tcagccgcgc gctggctgcc ggccgaaagc tgcacctgct
     1081 tgtggccgac gacagcccgg tcaatcagga agtggcccgc ggcctgttgg aactgcacgg
     1141 ccattcggtg gtgacggtcg gcaccggcca cgaagcgctg gaagcctggg agcgggaggc
     1201 gttcgacgtg gtcctgatgg acctcgaaat gcccgacatg gacgggctcg aagctgccga
     1261 gcgcatccgc cgcgtggaag cgagcaccgg ccgctacacg ccgatcatcg ctctcacggc
     1321 tcacgtgctg aaaggcgtcc gcgagcgctg cctggcggcc ggcatggatg actgcgtcac
     1381 caagccgctc ctccccgacg acctcctccc ccgcatcgcc gctttggccg atttgcactc
     1441 tcgttccgcc gctggccgca cggcggcggt cgcggcaaac taaggcgtgg accgctgttg
     1501 gaatcctgcg ctaccagttg tccttttctc cgggcggcct taacgctagg gtcacaaggg
     1561 caagcatgcc catgcaaatt gcgtagggca cagtcgggca acaaaggccc gaagtcaatg
     1621 cctgccctaa cgcgatctca aacagctcat ccttcctgtc agcgtaaagc gcttgatagt
     1681 gttccgtggt ggccgctcga taagccagca cgccccacat cgggatcgcg acaatccaga
     1741 cgaccgttgc agcaaccatg ccataattca cccatcgctg cagtactagc ttgttcccca
     1801 tgtttcgcat cgtcctccat cgcgcacgga aaggcaatca ctaggcagga caaggggtcg
     1861 cgcttcaaaa gagatctcgc atttttcctt ggaagatttc gggaataaat gaacgtccgt
     1921 gcgcatctat aggaatgggt cgaagtgcgc gccagcgata gaccctgcca tcgtcgtgcc
     1981 tgactgtcct tgggcgggtg gcaaggttct ggtttgctcg ccacaatcct gactgaagag
     2041 cgcaacagcg caacatcctc agtcgggggg tgttgcgcgg cgcaa
//
LOCUS       DASUGB010000090         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1817694, whole genome shotgun sequence.
ACCESSION   DASUGB010000090 DASUGB010000000
VERSION     DASUGB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817694"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFV87_01720"
     CDS             <1..505
                     /locus_tag="VFV87_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HEX5102497.1"
                     /translation="SLYGYSKCYALTGSREWLDVAERNARYWLDHLPADRVPYWDFHA
                     DLSQPPPLGRQKETSAGAIAASGLLDLARQTEYPERAHEYQQTALAMLDALVQPEYLA
                     SQTPGWEGILKHGVYHTAKNLGVDESVMWGEYFFVEALTTAVTHSAGRVSPDALARNR
                     QRYELLA"
     gene            complement(512..1378)
                     /locus_tag="VFV87_01725"
     CDS             complement(512..1378)
                     /locus_tag="VFV87_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102498.1"
                     /translation="MRRPNRFAFTLVELLVAIAIIGILIAILLPAVQMARESGRAASC
                     KNNLKQIGLALHSYHDTHGQFPAGWSADAPEGLPGWGWSSAILPFLEQQPLDRQIIRE
                     LPIEDPANQLARETLIRNFLCPSDGTRAKLHTIATGGFPGDDVDEGTPLFPVACSNYP
                     GNFGTFEIEDAPSQGDGVFFHNSQILMASIEDGLSNTIVVGERDAALGASVWVGMISQ
                     ANEPMARVVGVADHTPNHPVHHFDDFSSKHPGGVQFLMGDGSVRRYNDSIELSIYQAL
                     MTRNGKEAVPPP"
     gene            1633..>2630
                     /locus_tag="VFV87_01730"
     CDS             1633..>2630
                     /locus_tag="VFV87_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX5102499.1"
                     /translation="MSDLYHECGIAAVYHLPGDAHPLCPDGGPEEISRLLPRMLLDIQ
                     NRGQLAAGMTSYNPNRDQLLETHKDIGTVTEVFRLSHRGKAESLMRQYAGRAAIGHVR
                     YATCGSEDRNYAQPFERPHLEKRKWFTFGFNGQLANFQDLKEKLLSEADHHLARENDT
                     EIIMHEISRELSGDRRPRLVDVMRSVSQRFDGAYSLVLLNAQGDLLIARDPLGIKPLC
                     YVKEGNLFAAASESVPLLNLGFDSANIHSLPPGCAITIAGGEFKIERFAPEQKPAHCY
                     FEWIYFANVASTLDGRSVYLSRTALGEELARLEMADGSVPIDEDTIVVPVPDTSKAA"
BASE COUNT          496 a          812 c          859 g          463 t
ORIGIN      
        1 gtcgctctat ggctacagca agtgctacgc cctgaccggc agccgcgagt ggctcgacgt
       61 ggccgagcgc aacgcccgct actggctcga ccacctgccg gccgaccggg tgccgtactg
      121 ggattttcac gccgaccttt cgcagccgcc gccgctgggg cggcagaagg aaaccagcgc
      181 cggcgcgatc gccgccagcg gcctgttgga cctggcccgc cagacggaat atcccgagcg
      241 ggcccatgag taccagcaaa ccgccctggc gatgctcgac gctctggtgc agcccgaata
      301 cctggccagc cagaccccgg gctgggaggg aatcctgaag cacggggtct atcacacggc
      361 gaaaaacctg ggggtcgatg aatcggtgat gtggggcgag tacttctttg tcgaagccct
      421 cacgacagcc gtcacgcaca gcgcgggccg cgtttcgccg gacgccctcg cgcggaatcg
      481 gcagcgttat gaattgctgg cgtagattcg gctacggcgg cggcacggct tctttgccgt
      541 tgcgggtcat cagggcctgg tagatgctca gctcgatgct gtcgttgtac cggcggacgc
      601 tgccgtcgcc catgagaaat tgcaccccgc cggggtgttt gctggaaaag tcatcgaaat
      661 gatggacggg atggttcggc gtgtgatcgg ccacgccaac gacgcgggcc atcggctcat
      721 tggcctggct gatcatgcca acccagaccg aggcgccgag cgccgcgtcg cgctcgccca
      781 cgacgatcgt gttgctcagg ccgtcctcga tgctggccat gaggatctgg ctattgtgga
      841 agaagacgcc gtcgccctgg gagggagcgt cttcgatctc gaacgtgccg aagttgccgg
      901 gatagttgct gcaggcgacg gggaatagcg gcgtgccttc atcgacatcg tcaccgggaa
      961 acccaccggt ggcgatcgtg tgcagcttgg cgcgcgtgcc gtcggaaggg cagaggaagt
     1021 tcctgatgag cgtttcgcgg gccagttgat tcgccgggtc ctcgatgggc aattcgcgaa
     1081 tgatctgccg gtccaagggc tgctgctcaa ggaaggggag gatggcgctc gaccagcccc
     1141 agccggggag gccctccggc gcatcggcgc tccagccggc aggaaactgg ccgtgcgtgt
     1201 cgtggtagct gtgcagcgcc agcccgatct gcttgagatt gtttttgcag gaagcggccc
     1261 ggccggattc gcgggccatc tgcactgccg gcagcagaat cgcgatgaga atgccgatga
     1321 tggcaatcgc cacgaggagt tcgacgagcg tgaacgcgaa gcgatttggg cggcgcatga
     1381 caagtggctc ccggcgacga atcgaagaaa gacaacgaaa cgctaagtgg caacgtactt
     1441 ctaatgcaat gtaattgcaa gtagattgcg tgggcgttgt gtgaagattg gtgcaattcc
     1501 agcccgaagt ctcacgactt cggctgcaat acgccgggca acttgttgaa attggcctgg
     1561 gtccttagaa ttcaatagat cgggttcccc acccccgcag gcggcgaccg gattcgccga
     1621 acccacgctt cgatgagcga tctctatcac gagtgcggca tcgccgcggt ttatcacttg
     1681 ccgggcgatg cgcacccgct ctgcccggat ggcggcccgg aggagatttc gcgcctcttg
     1741 ccgcggatgc tgctcgatat tcagaaccgg gggcagcttg ccgctgggat gacaagctat
     1801 aaccccaatc gggatcagct cttagagacg cacaaggaca tcggtacggt caccgaggtt
     1861 tttcgccttt cgcaccgcgg caaagccgaa agtttgatgc ggcaatacgc ggggcgggcg
     1921 gcgattggcc acgttcgcta tgccacctgc ggctccgagg accggaacta cgcccaaccg
     1981 ttcgagcggc cgcatttgga aaagcgcaag tggttcacgt tcggctttaa cggccagctt
     2041 gcgaattttc aggacctcaa ggagaagctg ctcagcgagg ccgaccacca cctggctcgc
     2101 gagaacgaca ccgaaatcat catgcacgaa atcagccgcg agctgtcggg cgaccgccgc
     2161 ccgcggctgg tggacgtgat gcggagcgtc agccagcggt ttgacggggc gtacagcctg
     2221 gtgctgctca atgcccaggg cgacctgctc atcgcccgcg acccgctggg catcaagccc
     2281 ctgtgctacg tcaaggaagg caacctgttc gccgctgcca gcgagagcgt gccgctgctc
     2341 aacctggggt tcgactccgc caacattcat tcgctcccgc cgggatgcgc gatcacgatc
     2401 gcgggcggag aattcaagat cgagcgcttc gcacccgagc aaaagccggc ccattgctac
     2461 ttcgagtgga tttacttcgc gaacgtcgcc agcacgctcg acggccgcag cgtctatctc
     2521 tcgcggaccg cgctcggcga agagctggcc cggctggaga tggccgacgg cagcgtgccg
     2581 atcgacgagg acacgatcgt cgtccccgtg cccgacacca gcaaagccgc
//
LOCUS       DASUGB010000091         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1678247, whole genome shotgun sequence.
ACCESSION   DASUGB010000091 DASUGB010000000
VERSION     DASUGB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678247"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..483)
                     /locus_tag="VFV87_01735"
     CDS             complement(139..483)
                     /locus_tag="VFV87_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102500.1"
                     /translation="MRSQLSLALLFVFLSGCEPPQAPPGSAPAPPGAAPTMSATSVWS
                     QTQVEEFFKEEFQFTTIALKSTGGHNYQGTGVDPEGLTYTLTFQQVPGGYKAEWSTES
                     GNGAITFGNPVP"
     gene            917..>2054
                     /locus_tag="VFV87_01740"
     CDS             917..>2054
                     /locus_tag="VFV87_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5102501.1"
                     /translation="MSVSIPEFWDLLRDSRLLAAEQVQQLAMDYGSVKGSAEQGNAKT
                     LAQWLVARNVLSKYQTTILLAGRPGPFYYGDYKVYDRVEKGRLAGQFRAVHAPTGHPV
                     LLQFLTGPVISDADLWAAAANETLAACSVVSPHVQRFFEPVDLQSFKFLVSEDLRGDA
                     VDQRLAVGRFPAPEAARIVRLAAIGLAQMHQCGRVHGDVRPLNVLLESIPNHPGNVKL
                     LFDPTQPPEPFDFTQQQPDGRLAKMADYLAPELMSPGRVPDPLSDIYALGCTLYAMLS
                     GNPPFAGGNIQQKMTRHASEAIRPLEGFGVPQPLAQLVTYMMAKNPQVRYQSAAIVAE
                     QIAAFVDPNVLYAQPPQPAATLGSFEQFVRQKHAALSAPKKPAGM"
BASE COUNT          367 a          616 c          695 g          376 t
ORIGIN      
        1 tgaggtccaa aatgtcggca gcacgatcga agaacagctg aacggcttca tgcgacttca
       61 tcagcgcggg ctccgggaag gggaacggcc taggaaattc tagcccgcgg gaaaggtcgc
      121 ggtgaggtcg ttcgacgact acggcaccgg gtttccgaag gtgatggcgc cgttgccgct
      181 ctccgtgctc cattcggcct tgtacccgcc cggcacctgt tggaaggtca gcgtgtaggt
      241 caggccttcc ggatcgacgc cggtcccctg gtagttgtga ccgcccgtgg acttcagcgc
      301 aatagtcgtg aattggaact cttctttgaa gaattcctcg acctgtgttt gactccacac
      361 gctcgtcgca ctcatggtgg gagcggcacc tggcggcgcc ggtgcagatc ctggcggtgc
      421 ctggggtggc tcgcatccgc tgaggaaaac gaacagcagc gctagagaca gttgggagcg
      481 catggtggcg tcctagatca gaccctcaag tagctctcga ttttccaaca cctactgcgg
      541 ccgaccgttg tggtcaggaa gcttgacgga ggcgtcgatg cctcagtgaa atctacccgc
      601 tgctcgacga tcacgcgatc cactccacgg ccctgagtat tcatgcatcg cgcgacagcg
      661 tttgcgaaaa attcaccaga ctggccgaat tcgtccaagg gcccaagccc aggcttaaca
      721 tttgctttcc tgccggcgac cggggcgata gactgggagg tagattggaa aggggcaggg
      781 atcgcgttgc gcgcccttgg gcagcggcgg cgatgacctt tgccgcatag taacccgcag
      841 cgtacgcgag ggagttcctc gcttacgccc agggttgccg tcgaggggaa agatcgggcg
      901 aggaacagga gcagccgtga gcgtttcgat accggagttt tgggacttgc tgcgggacag
      961 ccgactgttg gcggccgagc aggtgcagca attggcgatg gactatggga gcgtcaaggg
     1021 ttcggccgag caggggaacg ccaagacgct ggcccagtgg ctcgtcgccc gaaacgtgct
     1081 gagcaaatat cagacgacga tcttgctggc cggtcggccg gggccgtttt attacggtga
     1141 ctacaaggtc tatgaccgcg tcgagaaggg acggctggcg ggacagttcc gggcggtgca
     1201 cgctccgacc gggcatcccg tgctgctgca gtttttgacc gggccggtca ttagcgatgc
     1261 ggacttgtgg gccgcggcag cgaacgagac gctggccgcg tgcagcgtcg tctcgcccca
     1321 cgtgcagcgg ttcttcgagc cggtcgatct gcagtcgttc aagttcctgg tgagcgaaga
     1381 cctgcgcggc gacgcggtcg accagcggct ggcggtgggg cggttcccgg cgccggaggc
     1441 ggcgcggatc gtgcggctgg cggcgatcgg cctggcccag atgcaccagt gcggccgcgt
     1501 gcatggcgac gtccggccgc tgaacgtgct gctggaatcg attccgaatc acccgggcaa
     1561 cgtcaagctg ctcttcgatc cgacgcagcc gcccgagccc ttcgatttca cccagcagca
     1621 gccggacggg cggctggcga agatggccga ttatctcgcg cccgaattga tgagcccggg
     1681 ccgcgtgccc gatccgctgt cggatattta tgccctgggc tgcacgctgt atgcgatgct
     1741 ctccggcaat ccgccgtttg ccggcggcaa tatccagcag aagatgaccc ggcacgcgag
     1801 cgaggcaatt cgcccgctgg agggcttcgg cgtgccgcag ccgctggcgc agctcgtcac
     1861 gtacatgatg gccaagaacc cgcaggtgcg ttatcaatca gcggcgatcg tggccgagca
     1921 gatcgcggcg ttcgtcgatc cgaatgtcct ttatgcccag ccgccgcagc ccgccgcgac
     1981 gctcggcagt ttcgagcaat tcgtgcggca gaagcacgcg gcactgtccg cgccgaaaaa
     2041 gccggcgggc atgc
//
LOCUS       DASUGB010000092         9021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_566886, whole genome shotgun sequence.
ACCESSION   DASUGB010000092 DASUGB010000000
VERSION     DASUGB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566886"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFV87_01745"
     CDS             complement(<1..772)
                     /locus_tag="VFV87_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102502.1"
                     /translation="MHDWLAEQTRRNLAVRGQWQAFESHRQRVTALLAEVAGQHRRSN
                     LCLLGAGNCNDVNLQELIDCYEALHLVDCDGDALARGIERQGLGRRPSLTMLGEIDLL
                     GLADCPPSATSAELAAAALNHQPLQNFKPVEVVASLCVLSQLLEAAASRCPSRSASHL
                     ELLQAVRYRHLRLLVELLRPGGCGLLITDLVSSVSARDLDVVGDEQLPALVSRWVSEH
                     NFFTGLNPAVVLASLQDDPWMAAHVKNVQPLGPWKWDLG"
     gene            complement(820..2814)
                     /locus_tag="VFV87_01750"
     CDS             complement(820..2814)
                     /locus_tag="VFV87_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5102503.1"
                     /translation="MHVALRAFRLALRYPLALGSAIGLTFLIAALWGGNIAAVYPILE
                     VAFQGQSLQDWSAQRIAKCESTIAEHQAAIARLQNNPPPRPELRTRWQYELDTFNGRL
                     AAEKKALEGMRRLHPWIVRYLPRDPFQTVALLVALIVSATAFKNACIVANLVLTSHVA
                     RRVTMDLRQQFFERVLRCDMRLFSQRSTGQLWPRFIEDIPHVAHGITTVFGRAIGEPL
                     KIFACLVGAGLISWRLLLVSLVLAPPAMLLIGALSRSLRRVTMRAYDKDVHLNGLLFE
                     TLQGLPIVQAFTMETAERQRLDCAALRSSQHMRRVAWLQALTKPAVEVLGIGAVSVAL
                     LAGAYLVLNQQTHLLGFKISDRPLSLSALLIFYAMLLGASEPLRRLSDVLPTFQLSLA
                     AAERLFTVLDEPPTIVEPRQARSVARPHQCLELDDVSFHYTAKRPVLRGVSLKISHGE
                     VVAIVGANGCGKSTLVNLIPRFYDTVKGAIRLDGISLSEMSLRDLRERIGIVTQHSHL
                     FDNTVLENIRYGSPGASDEQVFQAARQAFADEFITTQLAHGYETRVGHAGASLSGGQR
                     QRIALARAILRNPEILILDEPTSQIDLVSERLIQQSLQEFAQGRTVILITHRLSLLAL
                     ARRILVMQRGQIVAEGTQEELLNSSPLFREICDGRSRKAA"
     gene            complement(2933..4765)
                     /locus_tag="VFV87_01755"
     CDS             complement(2933..4765)
                     /locus_tag="VFV87_01755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5102504.1"
                     /translation="MLSSFSIILPVADASRGADDADYSIATESDVPAQYVVVASEPRP
                     ADLAPLVTGSRSVLIERSGISETEAINLGLQHSEGSIVGWLRAGCSHLDHTLKAAARY
                     FRQHPDTDVVCGDALWLDQRGKVLGEFRTRNVTRARLERGCCLFEAAVFLRRRVIDRH
                     GLLDPRWQYWSDYEYWLRLHRAGVRFGRLPRLLATCRVPGEPSHLGVMPRERTSEGLA
                     ELNDLLHHTLGRVPTRWIVQFGRVAAKERSAQDSRPPNQSHSPWHLSLTAHRHWNADP
                     WWSPRMWLDLPARFLVSYWRTLLRRPDVIRAVAPQFAAKFADRYLKRRIFKLRNHDPR
                     PLRIPPSYAHARPPLNPPLISIVTPNLNQGAFLEATLRSVLDQDYPRLEYIVQDGGSS
                     DHSVEVIHRYAPRLKRWASESDQGQADAINRGMQHASGDILAYLNSDDLLMPGSLAYI
                     ARYFAEHPSVDIVYGHRVLIDEQGDEIGRWVLPPHDESVLPLADYIPQETMFWRRSAW
                     ERVGARLDETFQFAMDWDLILRFRAAGLKFVRLPRFIGAFRISDSQKTQSLLATVGRL
                     EFDKCRERALGRVPSEREVRRAIRPYLRRHWWFDKLYLAGLIRY"
     gene            complement(4924..6219)
                     /locus_tag="VFV87_01760"
     CDS             complement(4924..6219)
                     /locus_tag="VFV87_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102505.1"
                     /translation="MRSCILCLPGILATLVTGFHSARGGENWPQFRGPTGLGYTEEKN
                     LPLEWGGTEQRNVLWSAPLTGQGHASPVVWGQRVFVCTAFWPQGVADRAKAIPEQHVA
                     CFRSTDGSRLWDVQVPPGPWLRSDFRSGPGGGYAAPTPATDGQRLFVMFGSSVVAALD
                     FDGKILWRKEIVPHSFDVTVGSSPVLFEKNVLVLCAMATKDDSRLVAFEQATGNVAWE
                     SRLPEMGFAHSTPVIVNVAGQPQMLVAASGGGRSSKGLMSLQPAGGQLLWWCQAGGEA
                     ASVAYRDQVVYVDSGRGGPGVAVDATGSGDVTATHIKWTVDQVPEGIGSPIIVGDYVY
                     RLHTPGILKCWQIADGKQVYAERLNGISSTWASPVADAAGRLYFATAGKSFVIQSGPE
                     FERLAENDLADANHASPAIAAGKIYLVGEKRIYCVGSRN"
     gene            complement(6249..7262)
                     /locus_tag="VFV87_01765"
     CDS             complement(6249..7262)
                     /locus_tag="VFV87_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102506.1"
                     /translation="MNARARLTALTALLGLALAARISRADDSETEKWVPISEGVTTQL
                     VNDGKKIGYPGLTAGVAVDRTTGDLFMVVCDQGVWKSSDQGRTFARVDEGAIGGRCET
                     GYAFDPAPAGKGLAAFMVYGGCGLSRDGGKSWIKFKSSHFDCGAVDWEAGGKTLLAIR
                     HESGGTLAYSTDEGQSWTDLGKGFTNGVGLFDARTLVATKGRGMLRSDDAGANWIEVS
                     DLQVVGGTMYVANNVGYWCTEKGLAITRDKGVTWSVPGAAILATHGPYFGRDEQHLVI
                     GSPDGLFESKDGGGSWEKIAPLPEGFTIRKSGQSYANFAWDPLNNILYASTMGKPTYR
                     WRR"
     gene            complement(7424..>9021)
                     /locus_tag="VFV87_01770"
     CDS             complement(7424..>9021)
                     /locus_tag="VFV87_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5102507.1"
                     /translation="GWRGANLNNILDFERDFPRVKVVRLEQNYRSTKSIVRVADALIR
                     HNVRRKQKDLFTENPEGSAVKLTAYPSQLDEALDIAGQISQQVRAEKRRPRDFAIFYR
                     TNALSRQFEHALREHVIPYQIVNGLEFYQRKEIKDILAYLHLLNNSRSDVALLRIINT
                     PTRGIGKATVDRLRDFARMKGLPLLEAARRAGVIEGMPKKAAVAVAKFVAIYDRLGEA
                     VHGAVEEIVGRVLAESGYREVLENSEAEEDQDRLANIEELLTAAREFDVKHPEGNSLE
                     QFLEESSLVADTDAWETETDKVTLMTLHAAKGLEFPAIFIVACEEGLLPHDRSRYDEE
                     KLEEERRLLFVGITRAKVDLQLSYAQYRSFRGLSAPTVASSFLMELPRHEMDLSNSVT
                     SRRMRPEFDEAHQGEAADDFEEGDIEFDVSQLDRRVGSAHQSTEDEEEGGHSPPYTKP
                     AKPLTSGLVTGADLLAQQTRPRVPPSRFKHGMVVEHPEYGAGTIVALSGEGAKRNAVV
                     QFFADQSQRTFRLAFSDLVPAEG"
BASE COUNT         1697 a         2812 c         2921 g         1591 t
ORIGIN      
        1 ggccaagatc ccacttccag gggccgagag gttgcacgtt tttaacatga gcggccatcc
       61 aaggatcgtc ttgaagcgac gcaagtacca cggcaggatt caggccggtg aagaagttgt
      121 gctcacttac ccaacgtgaa acgagcgccg gcaattgctc atcgccaacg acatcgaggt
      181 ctcgggcgga aacggacgaa accaggtccg tgatcaagag cccacagcct ccgggacgga
      241 gcagctccac cagcaatcgc aagtggcgat accgcaccgc ctggagcaac tccaggtggg
      301 aagccgatct gctcgggcac cgtgaggccg cggcctccag cagttgactg agcacgcaga
      361 gcgacgcgac gacctcaacg ggcttgaaat tctgcaacgg ttgatggttc agggcagcag
      421 ccgccaattc ggccgatgtg gccgaagggg gacaatccgc aagccccagc aaatcgatct
      481 cgcccagcat cgtcagcgaa ggtcgccggc ctaaaccttg acgttcaatt ccccgggcca
      541 aggcatctcc gtcgcaatcg acgaggtgaa gtgcttcata gcagtcaatc agctcctgca
      601 gattgacatc gttgcagttt cccgcgccca ggaggcagag attcgaccgg cggtgttgcc
      661 cggcaacttc agcaagcagc gcggttaccc gctgccgatg agactcaaat gcctgccact
      721 gcccgcggac ggcgagattg cgacgcgttt gctcggccaa ccaatcgtgc atatctggca
      781 ttgtagcccc gtactcgggg aggcaggcca gagtgggagc taggcagctt tgcgagacct
      841 tccatcgcag atttcgcgga ataatggcga cgaattcagc aactcttcct gcgttccctc
      901 ggcgacgatt tgcccgcgct gcatgacgag gatccgccgg gccagcgcca gcagcgacag
      961 gcggtgagtg atcaggatca cggtgcgtcc ctgggcgaat tcctgcagcg attgctgaat
     1021 cagccgctcg ctgacgaggt cgatctggct ggtcggctcg tcgaggatca ggatttccgg
     1081 attgcggaga atggcccgcg ccagggcgat tcgctggcgc tgaccgccgg agaggctggc
     1141 gccggcatgt cccacgcgtg tttcgtagcc gtgggccaac tgcgtggtga taaattcgtc
     1201 ggcgaaagcc tgacgggcgg cctgaaacac ctgctcgtcc gacgcgcctg gcgagccgta
     1261 gcggatattc tccaacaccg tgttatcgaa caggtgcgag tgctgcgtca cgatgccgat
     1321 ccgctcgcgc aggtcgcgga ggctcatttc gctgagactg attccatcga ggcggatggc
     1381 gcctttcacg gtgtcgtaaa agcggggaat caagttgacc agcgtcgatt tgccgcaccc
     1441 gttggcgccg acgatcgcga cgacttcacc gtgggaaatc ttcaggctga caccgcgcag
     1501 cacgggacgt ttggccgtgt aatgaaacga gacgtcgtcc agctccaggc actggtgcgg
     1561 acgcgcgacg ctgcgcgcct ggcgcggttc gacgatcgtg gggggctcgt caaggaccgt
     1621 aaagagccgc tcggccgcgg ccaggctgag ctggaaggtg ggcaggacat cggacagtcg
     1681 gcggagcggc tcgctggcgc ccaggagcat agcgtagaag atgaggagtg ccgaaaggct
     1741 tagagggcgg tcggagattt tgaaacccag gagatgggtc tgctgattca agaccaggta
     1801 ggcgcctgcg agcagggcga cggaaacggc gccgattccc agcacttcca ccgctggctt
     1861 ggtgagggcc tgcagccagg cgacgcgccg catgtgctgc gaactgcgga gggcggcgca
     1921 gtcgagtcgc tggcgttccg cagtttccat cgtgaaagcc tgcacaatgg gcaacccttg
     1981 cagcgtttcg aagagcaggc cattgagatg cacgtccttg tcataggccc gcatcgtcac
     2041 ccgccgcaag ctgcgcgaga gcgccccgat cagcagcatg gcgggcgggg ccagcaccag
     2101 ggaaacgagg agcagtcgcc aactaatgag ccccgctccg accaggcatg cgaaaatttt
     2161 gagcggctca ccgatcgcac gtccgaaaac ggtggtgatt ccgtgggcga cgtggggaat
     2221 gtcttcgata aagcgcggcc aaagctggcc cgtgctccgc tgggaaaaaa ggcgcatgtc
     2281 gcaccgcaga acacgttcga agaattgctg ccgcaagtcc atcgtgacgc gccgggcgac
     2341 gtgcgacgtg agcaccaggt tggcgacgat gcaggcattc ttgaatgccg tagccgacac
     2401 gatcagcgcc acgagcaagg cgacggtctg aaacggatcg cgcggcaagt agcgcacgat
     2461 ccatgggtgc agacggcgca tcccctcgag cgcctttttc tcggcggcga ggcggccgtt
     2521 gaaggtgtcg agctcgtact gccagcgcgt gcgcagctcg ggccgcgggg gagggttgtt
     2581 ttgcagcctg gcaatggcgg cctggtgctc ggcaatcgtc gattcacatt tggcgatccg
     2641 ctgcgcggac caatcctgca gcgattgccc ttggaaagcg acttccagaa tgggatagac
     2701 ggcggcaatg tttccgcccc acagcgccgc gatcagaaac gtcagtccga tcgcggagcc
     2761 cagcgccaag ggatagcgca gcgctagtcg aaaggctcgg agggcgacgt gcatggggcg
     2821 ggcaggtgag tgcgtgagtg gtgagtgcgt gagtgcgtga gtggtgagtg gtgagtgcgt
     2881 gagtggtgag tggtggcatt ggaaaccact caccactcac ttactcaccc gactagtaac
     2941 gaatcaaccc cgccaggtac agcttatcga accaccagtg tcggcggagg taaggccgga
     3001 tcgcgcggcg gacttcacgc tcggatggca cgcggcccaa ggcgcgctcg cgacacttgt
     3061 cgaattccag gcggcccacg gtggccagga ggctctgcgt cttctgggag tcgctaattc
     3121 ggaaggcgcc gataaaccgc ggcaagcgca cgaatttcag gcccgcggcg cgaaatctca
     3181 ggatcagatc ccaatccatc gcgaattgga aagtttcatc cagacgggct ccgacccgct
     3241 cccaggcact gcgccgccag aacatcgttt cctgcggaat gtagtcggcc agcggcagca
     3301 ccgattcatc gtgcggcgga aggacccagc ggccgatttc atccccttgt tcgtcgatga
     3361 gcactcggtg accgtagacg atatcgacgc tcggatgctc ggcgaaatag cgggcaatat
     3421 aggccagcga acccggcatc agcaggtcgt cggagttcag ataagccagg atatcgccgc
     3481 tggcatgctg cattccccgg ttaatagcgt cggcctggcc ctggtcggac tcggaagccc
     3541 agcgcttgag ccgcggcgcg tagcggtgga tgacttcgac gctgtggtcg ctggagccgc
     3601 catcctggac aatatattcc agccgcggat agtcttgatc cagcacgctg cggagcgtgg
     3661 cttccagaaa ggcgccctgg ttcaggttgg gcgtgacgat cgaaattagc ggtgggttca
     3721 gggggggacg ggcatgggca taggaaggcg ggatccgcag ggggcgcgga tcgtggttcc
     3781 gcagcttgaa gatccgccgc ttcaggtagc gatcggcgaa cttcgcggcg aattgcggcg
     3841 ccacagcgcg gatgacgtcc gggcggcgga gcagcgttcg ccagtaggac acgagaaacc
     3901 gcgccggcag gtctaaccac attctgggcg accaccaagg atcggcgttc cagtgtcggt
     3961 gggctgtcag cgacaaatgc cagggagaat gagactggtt ggggggacgc gagtcttgag
     4021 ccgatcgttc cttcgcggca acgcggccga actgcacgat ccaccgcgtc ggcacgcggc
     4081 ccagcgtgtg gtgcaacagg tcgttgagtt ctgccagccc ctcggacgtc cgttcacgcg
     4141 gcatcacgcc caggtggctg ggctctccag ggacgcggca ggtggccaaa agccgcggca
     4201 gccgaccgaa gcggaccccc gcgcggtgca gtcgcagcca gtattcgtag tccgaccaat
     4261 actgccagcg cggatcgagc aagccgtgac ggtcgatcac ccgccggcgc aagaaaacgg
     4321 cagcttcaaa gaggcagcag ccgcgctcga gccgggcgcg cgtgacgttg cgcgtgcgga
     4381 attcgcccag gactttgccg cgctgatcga gccacagggc atcgccgcaa acaacatcgg
     4441 tgtcgggatg ctgccggaaa taccgggcgg ccgccttcag cgtgtgatcg aggtgcgagc
     4501 agcccgcgcg cagccagccg acgatcgaac cttcgctgtg ctgcaggcca agattgatgg
     4561 cctcggtttc agaaatgcca gaacgctcga tcagcaccga gcggctgccg gtgacgagcg
     4621 gcgccaggtc tgccggtcga ggctcgctgg caacgaccac gtactgagcg ggaacgtctg
     4681 actcggtggc aatgctgtaa tcggcgtcgt ccgctccgcg gctggcgtcc gctaccggca
     4741 gaatgatcga aaaactggag agcatgcccg atttccaagc tgccgtgctt gccgcgtcac
     4801 cgttccaaaa actcgaaggg cgggaagcat agaaggccgg gcgatctgaa gcaatcgaga
     4861 acgcgagctg gcggcaaccc cgctagcaca gcccgccagc accggccgcc gagagcgcgg
     4921 ctatcaattc cggctgccga cgcagtagat gcgcttctcg cccacgagat agatcttccc
     4981 ggcggcaatc gcgggagaag catggttggc gtcggccaga tcgttttcgg ccagcctttc
     5041 aaactccggg ccggactgaa tcacgaagct ctttccggcg gtggcgaagt acagccggcc
     5101 ggcggcatcg gcgacgggac tggcccaggt gctgctgatg ccgttcagcc gctcggcata
     5161 gacctgcttt ccatcggcaa tttgccaaca cttgaggatg cccggcgtgt gcagacgata
     5221 gacatagtcg ccgacaatga tcggcgagcc gattccctcg ggcacctggt ccaccgtcca
     5281 tttgatatgc gtcgccgtca cgtccccgct gcccgtcgca tcgaccgcga cccccggacc
     5341 gccgcgtccg ctgtcgacat acaccacttg gtcgcgatac gcgacggaag ccgcctctcc
     5401 cccggcctgg caccaccaaa ggagctgtcc accggccggc tgaagcgaca tcagcccctt
     5461 ggaagaacgc ccgccgccgg aagccgccac gagcatctgc ggctgaccgg caacgttgac
     5521 gatgaccggc gtgctgtggg caaagcccat ttccgggagt cgggattccc aggccacatt
     5581 accggtcgcc tgctcgaacg ccacgagtcg cgagtcgtcc ttcgtggcca tggcgcagag
     5641 cacgagcacg ttcttttcga acaggacggg actgctgcca acggtgacat cgaacgagtg
     5701 cggcacaatt tccttgcgcc agaggatttt gccgtcgaaa tcgagggcgg cgacgaccga
     5761 cgaaccgaac atcacaaaca gccgctggcc gtcggttgct ggcgtggggg ctgcgtagcc
     5821 gccgccgggc ccgctgcgaa agtcggagcg gagccacggc cccggcggaa cctggacgtc
     5881 ccacagccgg ctgccgtccg tggatctgaa acaagcgaca tgctgctcgg gaatcgcctt
     5941 ggcgcgatca gcaactccct gcggccagaa cgccgtgcag acgaaaacgc gctgccccca
     6001 gacgacgggg ctggcatgac cctgcccggt gagcggcgca gaccacagga cattcctctg
     6061 ttctgtgccg ccccattcca gcggcaagtt cttctcctcg gtgtaaccca ggcccgtcgg
     6121 tccgcggaac tgaggccagt tctcgccgcc acgagccgaa tgaaagcccg tgaccagcgt
     6181 agcgaggata ccaggcaaac aaagtatgca gctccgcatg aatatctccc cgcaactgga
     6241 acctgtcctt agcgtcgcca gcgatacgtc ggcttgccca tcgtcgaggc atacagaatg
     6301 ttattcagcg ggtcccaggc aaagttggca tagctctggc cggacttgcg aatcgtaaag
     6361 ccctcgggca gcggtgcgat cttctcccac gatccaccgc cgtccttcga ttcgaaaagc
     6421 ccatccggcg agccgattac caggtgctgc tcatcccggc cgaagtatgg gccatgggtt
     6481 gccaggatcg ccgcgccggg cacgctccag gtcacgccct tatcgcgagt gatggccagg
     6541 cctttctcgg tacaccagta gccgacgttg ttcgcgacgt acatcgttcc gccaacaacc
     6601 tgcagatcgg agacctctat ccagttcgct ccggcgtcgt cgctgcgaag catgcctcgg
     6661 cctttggtag caaccaacgt ccgggcatca aacaggccca ctccattcgt gaagcccttg
     6721 cccaggtcgg tccaactttg cccttcatcg gtggaatagg ccagcgtccc gccgctctcg
     6781 tggcgaatgg ccaggagcgt cttgccgccc gcctcccagt ccacggctcc gcagtcgaag
     6841 tgcgaggatt tgaacttgat ccacgatttg ccgccgtcgc gcgaaaggcc gcagccgcca
     6901 tagaccatga aagccgccag tcccttgccg gccggtgcgg gatcgaatgc ataaccggtc
     6961 tcgcagcggc cgccgatcgc accctcatcg acgcgggcaa aggtccggcc ctggtcgctg
     7021 cttttccaga ccccctgatc gcagacaacc atgaacagat cgcccgttgt ccgatcgacg
     7081 gcgacgccgg ccgtcagccc cggatagccg attttcttgc cgtcgttgac gagttgggtt
     7141 gtcactccct cggagatcgg cacccatttt tccgtctcgg agtcgtccgc ccttgagatt
     7201 cttgccgcca gggccaaacc cagcagggcg gttagcgctg tcaatctggc gcgagcgttc
     7261 atcaatgaat cctccagcat ggcgcaggga cgcgtgtcag cttgcttcca gtaggtgatt
     7321 tcgtcgaatt gcatcatggg cccttgcacg cgacgtggta gggcaccgtg gatgacatgc
     7381 gtatcctgcg atagtccgac taggccggtc aagcgcttga ggcctaacct tctgccggca
     7441 caaggtcgct gaaggcgagg cggaaggtgc gctgggattg gtcggcgaag aactggacga
     7501 cggcgttgcg cttggccccc tcgccggaga gggccacgat cgtgccggcg ccgtactcgg
     7561 gatgctcgac gaccatcccg tgcttgaagc ggctgggcgg gacgcgcggg cgggtctgct
     7621 gagcgaggag atcggctccg gtgacgaggc cgctggtgag aggcttggcc ggcttggtgt
     7681 agggtgggct gtgcccacct tcctcctcat cttcagtgct ttggtgggca gagcccaccc
     7741 tacggtcgag ctgcgagacg tcgaactcga tgtcgccttc ctcgaagtcg tcggctgctt
     7801 cgccttgatg cgcttcatcg aattcgggcc gcatccgccg gctggtgacg ctgttagaaa
     7861 ggtccatctc atgccgcggt aattccatca ggaacgagct ggcgacggtc ggggcgctca
     7921 ggccgcggaa cgagcggtac tgggcatagc tgagctgcag gtccaccttg gcccgtgtga
     7981 tgccgacgaa cagcagccgc cgctcttcct ccagcttttc ctcgtcgtac cggctgcgat
     8041 cgtgcgggag caggccctct tcgcaggcga cgatgaagat cgccggaaac tccagcccct
     8101 tggcggcgtg cagcgtcatc agcgtgacct tgtcggtctc ggtctcccag gcgtcggtgt
     8161 cggccacgag cgacgactct tccaggaatt gctccagcga attgccttcg gggtgcttca
     8221 cgtcgaactc gcgagccgcc gtcaagagct cttcgatgtt cgccagccgg tcctggtcct
     8281 cctcggcttc gctgttttcg agcacctcgc gatagccgct ctcggccagg acgcgtccga
     8341 cgatttcctc gactgcgccg tggaccgctt cgcccagccg atcgtaaatg gcgacgaatt
     8401 tggcgaccgc gacagccgct ttcttgggca tgccctcgat cacgccggcc cggcgggccg
     8461 cttccagcag cggcagcccc ttcatccggg caaagtcgcg cagccgatcg acagtcgcct
     8521 tgccaatgcc gcgggtcggc gtgttaatga tccggagcaa cgccacgtcg ctccgcgaat
     8581 tgttgaggag gtgcaggtac gccagaatgt ccttgatttc tttccgttga tagaactcca
     8641 ggccgttgac gatctggtac gggatgacgt gctcgcgcag cgcgtgctcg aactgccgcg
     8701 agagggcgtt ggtgcggtag aagatggcga agtcgcgcgg gcggcgtttt tcggcccgca
     8761 cctgctggga aatctgcccg gcgatgtcga gcgcctcgtc gagctgcgag gggtacgcgg
     8821 tcagcttgac cgcggagccc tccgggtttt cggtgaacag gtccttctgc ttgcggcgga
     8881 cgttgtggcg gatcagcgca tcggcgacgc ggacgatgct cttcgtcgag cgatagttct
     8941 gctccaggcg gacgaccttg acgcgcggaa agtcgcgctc gaaatcgagg atgttgttca
     9001 ggttcgctcc ccgccagcca t
//
LOCUS       DASUGB010000093         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_150428, whole genome shotgun sequence.
ACCESSION   DASUGB010000093 DASUGB010000000
VERSION     DASUGB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150428"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFV87_01775"
     CDS             <1..208
                     /locus_tag="VFV87_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102508.1"
                     /translation="APQHGNARFAEQVFGLKFVDFHASKPRPVVADCGNRDHWSRLCK
                     SVEPRIIGHNCQIDNWVNALSGHN"
     gene            complement(205..951)
                     /locus_tag="VFV87_01780"
     CDS             complement(205..951)
                     /locus_tag="VFV87_01780"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX5102509.1"
                     /translation="MRRLRLPESLTVNSYLHEVGFEATSNGLRQLYADGPRHLIFADG
                     YLDERAGWQDHPTWHLPENAPATQIDIGGDGNTNCGHCGGRTHRLVRFAHVDHLPFVM
                     GVESLSIETCLSCLGWEDPRVMFYRHSDNGLPTCLAEHGGPPQFPAVALQQTTVQMVD
                     MGPRWHWQYWGATNSVENLHRIGGHPTWIQSAEYPQCPGCHSTMCFLLQLDSNLPTMD
                     EGDWLWGSGGIVYVFWCTACRISAMLWQCT"
     gene            1186..1416
                     /locus_tag="VFV87_01785"
     CDS             1186..1416
                     /locus_tag="VFV87_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102510.1"
                     /translation="MKSLHALQRSELDYGFCRFVFPVACERGDGVRDEFWPIVKRNVG
                     QCGILIRATLGQLIHNTASETQRIHGRTPRDG"
     gene            complement(1498..2763)
                     /locus_tag="VFV87_01790"
     CDS             complement(1498..2763)
                     /locus_tag="VFV87_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102511.1"
                     /translation="MIDRRSFLAASTALGLGAALARGQESPPKKRLAVITTLWRYKSH
                     AWHMAERFLHGYPREGKWHQPPLEVVSAYVDQRPEGDLSGKRAEEFGFTIYKSVAEAL
                     RCGGDKLAVDAVLLIGEHGDYPINEFGQKQYPRYELFSQITKVFQEDGRATAIFSDKH
                     LSWKFAWAKEMVETSKAMNFPFLAGSSLPVTWRMPAIDLPYGAEVEEIVGVAFGSLDI
                     YDFHALEMIQCMAERRKGGETGVVSMHALRGDAVWQALDSGSWKAGGWSPRLFEACLS
                     RSQTLAQDGVSSHRYPTPAQMREWTKEPVAYRFEYADGLKATMLQLNGLVEDFNFAAK
                     LKGEAEPLSTLFYLPPNPNVVYSAALMAKAEEMFLTGKAPYRVERTLLTSGLTEAGLQ
                     SLTLGKRLETPHLTKVRYAAPRESQFVRT"
     gene            complement(2801..>3339)
                     /locus_tag="VFV87_01795"
     CDS             complement(2801..>3339)
                     /locus_tag="VFV87_01795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5102512.1"
                     /translation="LTAAVISGLAVVGLNVWTGSYVPTIGASGAVMAVLMLYAIHYPR
                     STVLIFWFFPLEVRWLVVIYVIWDLHPLLLQLAGDQVYTGIAHAGHLGGLAFGFLYWK
                     FNLHLERWWNKLPFRMPASTYRPRETIPIRRSPQRELEDETDKVLKKIHESGESSLTD
                     AERRTLQRASERAKRRRE"
BASE COUNT          643 a         1055 c         1011 g          630 t
ORIGIN      
        1 tgccccgcag cacggcaacg cccgctttgc ggaacaggtc tttggcctga aattcgtgga
       61 ttttcatgcg tcaaagccgc gaccagtggt cgcggactgt gggaaccgcg accactggtc
      121 gcggctttgt aagtcagtag agccgcgaat tatcgggcac aattgccaaa ttgacaattg
      181 ggtgaatgct ctgtcgggac acaactaggt gcattgccac aacatagcgc tgatccggca
      241 cgcagtgcac cagaaaacat agacaatccc gcccgatccc cagagccaat cgccttcgtc
      301 catcgtcggc aggttcgaat caagctgcag caagaagcac atcgtggaat gacagccggg
      361 gcactgcggg tattccgccg attgaatcca ggttggatgc ccgccaatcc gatgcaggtt
      421 ctccacgctg ttggttgcac cccagtactg ccaatgccat cgcggcccca tgtcgaccat
      481 ctgaaccgtt gtctgctgaa gggcaactgc gggaaattgg ggcggtccgc cgtgctccgc
      541 gaggcacgtc ggcaacccat tatcggaatg acgatagaac atcacgcgcg gatcctccca
      601 gcccaggcag gaaaggcacg tctcgatcga cagtgactcg actcccataa caaacggcaa
      661 gtgatcgaca tgtgcgaatc gcaccagccg atgagttcga ccgccgcagt gcccgcagtt
      721 cgtgttgcca tcgcccccga tgtcaatctg cgtcgctggg gcgttctcgg gcaagtgcca
      781 cgttgggtgg tcctgccagc cggcacgctc gtctaggtag ccgtcggcga agatcaagtg
      841 acggggaccg tcggcataga gttgtcgcaa accattactt gtcgcctcaa atccaacctc
      901 gtgcagatag gagttgactg tcagagattc cggcaggcga agtcgacgca tctgcaagtt
      961 cagatcagcg catttcacgt gatcaagtgc ccagcgcagt aattccgaat tccgagtttc
     1021 cagcagacac atccaagcct tgcggcggcg ctcggctgaa ctcctccgat cgttggcggc
     1081 tttgcacagg tggtccgcaa cctggtcccc tgcacctcgc cagggccaag tttcgtacgt
     1141 cgaaccttcg tttggtgcca gctcgaacag gtccggcaaa tgcggatgaa gagcttgcac
     1201 gcattgcaac gaagcgaact cgactacggc ttctgccgct ttgtttttcc ggttgcgtgt
     1261 gaacgcggcg atggcgtgcg cgacgagttc tggccaatcg tcaagcgcaa cgtaggtcaa
     1321 tgcggcatcc tgatacgtgc cacccttggg cagctcatcc acaatacggc aagcgagacg
     1381 cagagaatcc acgggagaac gccgcgagat ggctagcgcc cgatccatcg gctcggcttc
     1441 ccagccgcgg cgctttacat cggcaacaaa gtcatcgata tcggtcatga gagcacccta
     1501 cgtccgcacg aactgcgact cgcgcggggc tgcgtagcgg actttcgtca ggtggggcgt
     1561 ttccagccgc ttgccgagcg tgagcgattg caggccggct tcggtgaggc cgctggtgag
     1621 gagggtccgt tccacacgat acggcgcttt gccggtcagg aacatctcct cggctttggc
     1681 catgagggcg gcggaataga cgacgttcgg attcggcggc aggtagaaca gggtcgagag
     1741 cggctcggct tcgcccttca gcttggcggc gaagttgaaa tcctccacga ggccgttgag
     1801 ctgcagcatc gtcgccttga ggccgtcggc gtattcgaag cggtaggcga cgggctcctt
     1861 cgtccactcg cgcatctggg cgggggtcgg atagcggtgg ctgctgacgc cgtcctgagc
     1921 cagcgtctgg ctgcgcgaca ggcacgcttc aaacagtcgc ggcgaccagc cgccggcttt
     1981 ccacgagccg ctgtccaggg cctgccacac ggcatcgccg cggagggcgt gcatgctgac
     2041 gacgcccgtt tcgccaccct ttcgccgctc ggccatgcac tggatcattt ccagggcgtg
     2101 gaagtcgtag atgtcgagcg agccgaacgc gacaccaacg atctcctcga cttccgcgcc
     2161 atacggcaga tcgatcgccg gcatccgcca cgtgaccggc agcgacgagc cggcgaggaa
     2221 ggggaaattc atggccttcg acgtttcgac catctccttg gcccaggcga atttccagga
     2281 gaggtgcttg tcgctgaaga ttgccgtggc ccggccatcc tcctggaaga ccttcgtgat
     2341 ctgcgagaac agctcgtacc gcggatattg cttctggccg aactcgttga tggggtaatc
     2401 gccatgctcg ccgatcagca ggaccgcatc gacggccagc ttgtcgccgc cgcaccgcag
     2461 cgcctcggca acgctcttgt agattgtgaa cccaaattcc tccgcccgct tgccgctcaa
     2521 atccccttcc ggccgctgat cgacatacgc gctgaccacc tccagcggcg gctggtgcca
     2581 cttgccttcc cgcggatagc cgtgcaaaaa ccgctccgcc atgtgccagg cgtgggactt
     2641 gtagcgccag agcgtcgtga tgacggccag gcgcttcttc ggtggtgact cttggccgcg
     2701 agccagcgcg gcccccaaac ccaatgcggt cgatgcggcg agaaacgaac ggcggtcgat
     2761 catggtgttg tcccctgtct cctgactcct gtcccctgcc ctactcccgc cgtcgcttcg
     2821 cccgctcgct ggctcgctgc agcgtgcggc gctcggcatc ggtgagcgaa gactcgcccg
     2881 actcatggat cttcttcagt accttgtcgg tctcgtcctc aagttcgcgc tggggcgagc
     2941 gacgaatcgg aatcgtttca cgcgggcgat aggttgacgc gggcattctg aatggcagct
     3001 tgttccacca gcgctccagg tgcagattga acttccaata gagaaagccg aacgccaggc
     3061 cgcccaggtg tccggcgtga gcgatgcccg tatagacctg gtcccccgcc agttgcaaga
     3121 gcagcgggtg caagtcccag atgacgtaga tcacgaccag ccagcggacc tccaggggaa
     3181 agaaccagaa gatgaggacc gtgctgcggg gatagtggat ggcgtacagc atcagcaccg
     3241 ccatcacggc gcccgaggcg ccgatcgtcg gcacgtagct tcccgtccag acattgagcc
     3301 ccaccaccgc caggccggag atgaccgccg cggtcaggt
//
LOCUS       DASUGB010000094         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_845440, whole genome shotgun sequence.
ACCESSION   DASUGB010000094 DASUGB010000000
VERSION     DASUGB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845440"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFV87_01800"
     CDS             complement(<1..699)
                     /locus_tag="VFV87_01800"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5102513.1"
                     /translation="MSAGPTFKDLRVAALESRNSAEIARLIEKFGGRPFVSPSMREVP
                     IEQNREAIDFANRLITGGIDVMIFLTGVGFRHLLAAVEKHVDRQRYLDSLSDIATICR
                     GPKPVAAMAAVGLKPTHRVPEPNTWRELLATIDGGISIANQNVGLQEYGITNRSLIAG
                     LEARGARVVNVRVYQWDLPVDTKPLEENIRAIAAGERDVLIFTSAHQVANLLRMAQQL
                     GLEPELRAALKTMAV"
     gene            849..2003
                     /locus_tag="VFV87_01805"
     CDS             849..2003
                     /locus_tag="VFV87_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102514.1"
                     /translation="MPSSDFDQLQSAFSSGGAEAALAQAAKLLREQKKYHELFEVLKM
                     QVRRKLDLPLLPGATDELPEETRAKLEDGLIASCREVGSALLQEGKIREGWMYLRPVG
                     DRAEAASLMGQIEPDEENYEDLIEVCLHEGVDIGRGYGLVLEHYGTCNAITQYDSSVV
                     RRPRAEQAPAAQRLLQRVHADLVHSVKADIARQEGQQPAAGTLKELLADRDWLFQDNA
                     YHLDTTHLAACVRISRVLSDPADLRMALDLTEYGRRLSQQFQYQGDEPFADIYPANAL
                     YFQALLGENIEEAIEHFKSKAEMLDPQYHGYAAIETYVDLLARLGRHDQALAAAVKHG
                     VTSIQPLGNAPPLVDLAQKSGQFQSVLDHCRAKEDVLGFAAALVQSSSAK"
BASE COUNT          375 a          653 c          638 g          366 t
ORIGIN      
        1 cacggccatc gttttcaagg ctgcgcgcag ctccggctca aggccgagct gctgcgccat
       61 ccgcaacagg ttcgcgacct ggtgggcgct agtgaagatc agcacgtcgc gctcgcctgc
      121 cgcgatcgcc cggatgttct cctccagcgg cttggtatcg accggcaaat cccactgata
      181 gaccctcacg ttgacgaccc gcgccccgcg ggcttccagg ccggcgatca ggctgcggtt
      241 ggtgatgccg tactcctgca agccgacgtt ctggttcgcg atcgagatgc cgccgtcgat
      301 cgtggcgagc agttctcgcc acgtgttcgg ctccgggacg cggtgagtcg gcttgaggcc
      361 gactgcggcc attgcggcga ccggcttggg gccgcgacag atcgtggcga tgtccgaaag
      421 cgaatccaga taccgctggc gatcgacgtg cttctcgacc gcggcgagca aatgccgaaa
      481 gccgaccccc gtcagaaaga tcatcacgtc gattccgccg gtaatcagcc gattggcaaa
      541 gtcgatcgcc tcgcggttct gttcgatggg cacctcgcgc atgctcgggc tgacgaacgg
      601 ccgcccgccg aacttttcga tcagtctcgc gatctctgcg ctattcctac tttctagcgc
      661 ggcgacgcgg aggtctttga aagtggggcc agcgctcatg atggcttcat gctacttggg
      721 cttcggaatc atcgacagag ggcgaatgca agcggtgttc ggcagcaggc atcgttggta
      781 cgatggacgc gtaccaagta cctggtacag cccgcccata tcgctgatca ctccgaagca
      841 gccctcccat gcccagctcc gatttcgacc aactgcagtc tgccttctcc agcggcgggg
      901 ccgaggcggc tttggcccag gctgcgaagc tgcttcgcga gcagaagaag tatcacgaat
      961 tgttcgaggt gctgaaaatg caagtccgcc gcaagctcga cctgccgctc ttgccgggcg
     1021 cgacggacga gctgccggaa gagacgcgtg ccaagctcga ggacgggctg atcgcgtcat
     1081 gccgcgaggt tggttcggcg ctcctccagg aaggcaaaat tcgcgagggg tggatgtatc
     1141 tgcgtccggt cggcgaccgg gcggaagccg ccagcctaat ggggcagatc gagccggacg
     1201 aggagaacta cgaggacctg atcgaggtgt gcctgcacga aggggtcgat atcggccgcg
     1261 gctatgggct ggtgctggag cattacggca cttgcaatgc gatcacgcag tacgattcgt
     1321 cggtcgttcg tcgaccgcgg gccgagcagg cgccggccgc ccagcggctc ttgcagcggg
     1381 tgcatgcaga cctcgtccac agcgtgaagg cggacatcgc ccgccaggaa ggccagcagc
     1441 cggcggctgg cacgctcaag gaactgctcg ccgaccgcga ctggctcttt caggacaacg
     1501 cctatcatct cgacacaacg catctggctg cgtgcgtgcg gatttcgcgc gtgctctccg
     1561 atccagccga cctgcgcatg gccctcgact tgaccgaata tggccggagg ctgagccagc
     1621 agtttcaata ccaaggcgat gagccgtttg ccgacatcta tcccgccaac gctctctatt
     1681 tccaggcgct cctcggggag aacatcgaag aggccatcga gcacttcaaa tccaaagccg
     1741 agatgctcga cccgcagtat cacggctatg cggcaatcga aacgtacgtc gatcttctcg
     1801 cccgcctcgg ccgccatgac caggctctcg ctgcggcggt caagcatggc gtcaccagca
     1861 tccagccgct gggcaacgcc ccgcccctcg tcgatctagc acagaaatcc ggtcagttcc
     1921 aatcggtgct cgatcactgc cgcgcgaagg aagacgtgct cggcttcgcg gcggcactgg
     1981 tgcagtcgtc aagcgccaaa tagccgggag caaacttgct cccgcgtggg gc
//
LOCUS       DASUGB010000095         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_705931, whole genome shotgun sequence.
ACCESSION   DASUGB010000095 DASUGB010000000
VERSION     DASUGB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705931"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..820)
                     /locus_tag="VFV87_01810"
     CDS             complement(2..820)
                     /locus_tag="VFV87_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5102515.1"
                     /translation="MQRVAGWKFGALVLGVFGAIWLVREVAVSFVPSAATSAVAQEAA
                     EEGATGDAAGGTTEAPPRRMSYLEFFFRALGIRYSLAFLVLSFTFVAYLVMNLLSLRR
                     DGICPRHLAAAFDANLNEKKFQEAFDLAKNDDSMLGQMLAAGMQNLQQGYDKALDSMG
                     QVGEEENMKLEHRLSFLSLVGSIAPMVGLLGTVDGMVATFQEIAIAGAQPKASDLAEG
                     ISMALITTLVGLILAIPAIIAFNIMKNRLTRLVMEAGQQAGNLMSRFETMGKQK"
     gene            complement(1220..>4456)
                     /locus_tag="VFV87_01815"
     CDS             complement(1220..>4456)
                     /locus_tag="VFV87_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102516.1"
                     /translation="YPGKHSGMREFWNRPSRPCPGTGMVSSRHFPEDWQGNFLNCNVI
                     GFQGIFRVKVTEEGSGLKGETLEDLVKSSDPNFRPTAVSCAPDGSIYFLDWQQQLIGH
                     LQHHIRDPNRDKSHGRIYRITYEGRPLLTPPAIDGQPIEALLELLKSPEDDVRTRAKI
                     ELGKHDADQVVAATKQWAIGLDKNDPEYEHHRLEALWVHQWHNVVDPELLQQVLASPD
                     HRARAAAAHVLCYWRDRIPAALDIFKRLAADPHPRVRLEAVRAASFYTVPEAVEVPLI
                     AAELPGDYYLDYCTSETMKALEPYWQAAVAENHTVNVTSPAGVRFFLGKMSLEQLLAL
                     DRSQPVYLELLYRPGVRDELRLEAIRGIAADDKKPELAVLLDAIRAIDNQQQTGEQSV
                     VFDLMRILAGRGAGDLAAARGELEKLALTARQAAIRQIAYVAMVSVDGSADVAWALAN
                     QSPGSLRDFLTAAPLIADPGQRAGLYDRIEPLLAGLPESLGGASAAKGTYGRFVRIEL
                     QHRRTLTLAEVEVFSEGQNVARRGKTTQKNTAHGGDASRGIDGNKDASYGGGGQTHTQ
                     ENTPDPWWEVDLGEEVPIDSIAIYNRGDGDLGQRLKGFTLRVLDAGRNEVFRKDDNSA
                     PALVSNFDIGGGGPEALVRRAAMLALVSVRGQEARTFGLLTPFITADTDRLAAIRAIQ
                     RLPRNTWPKEQAQPVLDSLVAYITEFPVADRTSPAALDALQLADALAALLPADQAKSY
                     RGKLRELGVRVIRLGTLPERMSYDQEVLVVQAGKPVEFLLENTDLMPHNFVITQPGAL
                     EEIGQAAEASAQQPEFARRHYVPQSPQVLLSSILLQPRQSQKLSFVAPTQPGVYPYVC
                     TYPGHYRRMHGALYVVPDLDAYLEKPDEYLASAKIEPVDPLLKDRRPRTEWKLEDLAA
                     AVSTIEGRNYAAARQMFQVASCVACHKLEGVGNAFGPDLSQLDAKLQPLDLVKEMLDP
                     SAKINEKFQTQIFQLDSGMTVTGLVVEETPTVIKLVENPLAKAAVTEIQVEEIVGRKK
                     SEVSIMPKGLLDKLTRDEILDLVAYIASRGKQDHPLFQAGGHAHHHGH"
BASE COUNT          757 a         1435 c         1432 g          832 t
ORIGIN      
        1 actatttttg cttgcccatc gtctcgaaac ggctcatcag gttgcccgcc tgctggccgg
       61 cttccatcac caggcgcgtc aggcggttct tcatgatgtt gaacgcaatg atggcgggaa
      121 tggccaggat caggccgacc agcgtcgtga tcagggccat ggaaatgcct tcggccaggt
      181 cggaggcctt gggctgggcg ccagcgatgg cgatttcctg gaacgtggcg accatgccgt
      241 ccaccgtgcc gagcaggccg accatcggag cgatcgagcc gaccagcgac aggaagctca
      301 ggcgatgctc cagcttcatg ttctcctctt cgccgacctg ccccatcgaa tcgagggcct
      361 tgtcgtagcc ttgctgcagg ttttgcatgc cggcggcgag catctggccg agcatcgagt
      421 cgtcgttctt ggccaggtcg aacgcttcct ggaacttctt ttcgttcagg ttggcgtcaa
      481 acgctgccgc caggtgacgc gggcagatgc cgtcgcgccg caggctgagc aggttcatga
      541 ccagataggc cacgaacgtg aacgacagca ccaggaacgc cagcgaatag cgaatgccca
      601 gagcccggaa aaagaactcc aggtagctca tgcgccgcgg cggggcttcg gtcgttccgc
      661 ccgcagcgtc gccggtggcc ccttcttccg cggcttcctg ggccacggcg gacgtggcgg
      721 ccgacggcac gaacgagacc gcaacttccc tgaccagcca gatcgcgccg aacactccca
      781 ggaccagcgc accgaatttc caacccgcaa cccgctgcat aaagagcctc cgctaccgca
      841 aaacctgcca ggaaagggaa tctgccacag tgcaattctt acattgccgc tattccagtg
      901 ccgctattac agccgccgct agtgaagttc tgcccgaagt gggaaaatta ttgcttcccg
      961 agttcaaccc ttcccaagcg ctgaatggcg ccagatgtac tcaggcgaat ctcgatcggc
     1021 ggggaatatc gagcctccat tctaatcacg cctgcgaatt ggggtaaagc accgactttg
     1081 accgttttta gggccggatt agggccgggt ctgaggcatc ccgtgccaat ttcgtttaac
     1141 gcgcagccgc aggcgtagcg gcttccaccc tgagagggct gctacgcctt cggctgcgcg
     1201 ttaaacgaac ggcccacgat caatgcccgt gatggtgtgc gtgccccccc gcctggaata
     1261 gcggatggtc ttgtttcccg cgggaggcga tataggccac taggtccaga atctcgtcgc
     1321 gcgtgagctt atccagcagc cccttgggca tgatcgagac ttcggacttc ttgcggccga
     1381 cgatttcctc aacctggatc tccgtcacgg cggctttggc gagcggattt tcgaccagct
     1441 tgatgacggt cggcgtttct tcgacaacga ggcccgtgac ggtcatgccc gaatcgagct
     1501 gaaagatctg cgtctggaat ttctcgttga tcttcgccga cgggtcgagc atttccttca
     1561 cgagatcgag cggctggagt ttggcatcga gctgcgacag atccgggccg aaggcgttgc
     1621 cgacaccttc gagcttgtgg caggcgacgc aactcgccac ctggaacatc tgcctggcgg
     1681 cggcatagtt gcggccctcg atcgtggaaa cggcggcggc gaggtcttcc aatttccact
     1741 cggtccgcgg ccggcgatcc ttcagcagcg gatcgaccgg ctcgatcttg gccgatgcca
     1801 ggtactcgtc gggcttctcc agataagcgt cgagatcggg cacgacgtag agcgccccgt
     1861 gcatccggcg gtagtggcca ggatacgtac agacgtacgg atacacgccc ggctgcgtcg
     1921 gcgcgacgaa gctcagcttc tggctctgcc gcggctgcaa gaggatgctc gaaaggagga
     1981 cctgcggcga ctgcggcacg tagtgccggc gggcgaactc cggctgctgg gcgctggcct
     2041 cggctgcctg gccgatctct tccaatgcgc ccggctgcgt gatgacgaaa ttgtgcggca
     2101 tcaggtcggt gttttcgagc aggaattcca ccggcttgcc ggcctgcacc acgagcactt
     2161 cctggtcata gctcattcgc tccgggaggg ttcccaggcg aatgacccgc acgccgagct
     2221 cgcggagctt cccgcgatag ctcttggcct ggtcggccgg caagagcgcg gcgagtgcgt
     2281 cggcaagctg cagggcgtcg agcgccgccg gcgaggtgcg gtcggcgacg ggaaactccg
     2341 tgatgtacgc cacgagtgaa tcgagcactg gctgggcctg ctctttgggc caggtgtttc
     2401 gcggcagacg ctgaatcgcc cgaattgccg ccaaacggtc ggtgtctgcg gtgatgaagg
     2461 gggtcaaaag gccgaacgtg cgggcttcct ggccgcggac ggagacgagt gcgagcattg
     2521 cggcccggcg gaccagcgcc tccggtccgc cgccgccgat atcgaaattc gataccagcg
     2581 cgggagcgga attgtcgtct ttgcggaaga cctcattccg gccggcgtcg agcacccgca
     2641 gcgtgaagcc cttcagccgc tggcccaggt cgccgtcgcc gcggttgtaa atggcgatcg
     2701 aatcgatcgg cacttcctcg cccagatcga cctcccacca cgggtcaggc gtgttttcct
     2761 gcgtgtgcgt ctgcccgccg ccgccgtacg atgcatcctt attgccgtcg atgccgcggc
     2821 tggcgtcgcc gccatgcgcg gtgttcttct gcgtggtttt gccgcggcgg gcgacgttct
     2881 ggccttcgct aaagacctcg acctccgcca gcgtcagcgt gcggcggtgc tgcagctcga
     2941 tccgcacaaa gcggccgtaa gtgcctttcg ccgccgatgc cccgccgagc gactccggca
     3001 agccggccag cagcggttcg atgcggtcgt agaggccggc ccgctggccg ggatccgcaa
     3061 ttagcggagc cgccgtcaga aaatctcgca gcgagccagg cgactggttc gccagtgccc
     3121 acgcaacgtc cgccgagcca tcgacgctca ccatcgcgac atacgcaatc tggcggatcg
     3181 cggcttgcct ggccgtaagc gcaagcttct ccagctctcc gcgggcagcg gccaggtcgc
     3241 ccgcgccgcg gccggcgagg attcgcatca ggtcgaacac gacgctctgc tctcccgttt
     3301 gctgctggtt gtcgatcgct cggatcgcat ccagcagcac ggccagttcg ggctttttgt
     3361 cgtccgccgc gatgccgcgg attgcttcca gccgcaattc gtcgcggaca ccgggccggt
     3421 aaagcagctc cagataaacg ggctgcgagc ggtcgagcgc gagcaactgc tccaggctca
     3481 tctttcccag gaagaaccgc acgccggctg gcgacgtgac gttgaccgtg tggttctcgg
     3541 ccaccgcggc ctgccagtaa ggctccagag ccttcattgt ctcgctcgtg caatagtcga
     3601 ggtagtaatc gccgggcagc tcggccgcga tcagcggaac ttcgacggct tcagggactg
     3661 tgtagaagct ggcggcccgg accgcctcca gccggacgcg cgggtgcgga tcggccgcga
     3721 gccgcttgaa gatatcaagc gcggctggaa tgcggtcgcg ccagtagcac aggacgtgag
     3781 ccgccgcggc ccgcgcccgg tggtcgggcg aggcgaggac ctgctgcagc agctccggat
     3841 cgacgacgtt gtgccactgg tggacccaca gcgcctccag ccggtggtgc tcgtactcgg
     3901 ggtcgttctt atccaagcca atagcccact gcttcgtcgc ggcgacgact tggtccgcgt
     3961 cgtgcttgcc cagctcgatc ttggcccgcg tgcggacgtc atcctcaggc gacttcagca
     4021 gttccaagag cgcctcgatc ggctggccgt cgatcgcggg cggcgtgagg agcggccggc
     4081 cttcgtacgt gatgcgatag atgcggccgt gcgacttgtc gcggttgggg tcgcgaatgt
     4141 gatgctgcag gtgcccaatg agctgctgct gccagtcgag gaagtaaatc gagccgtccg
     4201 gcgcacagct caccgccgtc ggccggaagt tcgggtcgct cgatttcacc aggtcttcga
     4261 gcgtctcccc cttcagcccc gagccctcct cggtgacctt cacccggaaa atgccctgaa
     4321 agccgatgac gttgcagttc aggaagttgc cctgccagtc ctccggaaaa tgccggctgc
     4381 tgaccatgcc ggtgccgggg caggggcgcg agggacgatt ccagaactcg cgcatgcccg
     4441 aatgcttgcc gggata
//
LOCUS       DASUGB010000096         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1678710, whole genome shotgun sequence.
ACCESSION   DASUGB010000096 DASUGB010000000
VERSION     DASUGB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678710"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFV87_01820"
     CDS             complement(<1..318)
                     /locus_tag="VFV87_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5102517.1"
                     /translation="MAHQDGHDHSHNGNGHAPAAFQPPASFEVRILRQDAPGQPSYWE
                     RHRVDYEPEMNVISVLQRIAARAKTVDGKAVAPVAWDCNCLEEVCGACTMVINGKVRQ
                     SCSA"
     gene            complement(353..2368)
                     /gene="sdhA"
                     /locus_tag="VFV87_01825"
     CDS             complement(353..2368)
                     /gene="sdhA"
                     /locus_tag="VFV87_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX5102518.1"
                     /translation="MPAKRIMIVGGGLAGLAAAMKLSELGIDVDLMSLVPVKRSHSVC
                     AQGGINSVNDLTRQQGDDEWLHFDDTVYGGDFLQHQPPVKEMAYWGPKIIDLMDRLGV
                     TFNRTPEGLLDRRRFGGTLYKRTAFAGATTGQQLLYALDEQVRRWETAGKVKKYEFWD
                     FLGPILDDNGVCRGAVGQDLVSMEIRAFPADAVIVASGGPGLIYGRSTMSMACTGSAA
                     SRCFQVGAKYGNGEFIQVHPTAIPGADKLRLMSESARGEGGRVWVPKTPQDPRDPLTI
                     PESERYYFLEERYPKYGNLVPRDIATREIFDICVNEGLSVEKDRQCVYLDLTHIPRKE
                     LDRKLGGILEIYEKFQGVDPRSTPMKIFPAVHYAMGGLWVDYQKSADGGLVAGSPRNQ
                     VTSIPNLYAIGECDYQYHGANRLGANSLLSCIFSGLFVAPGVESLLKGQKQAASELPS
                     SLFEQARKQHQSRHDALLKRSGGGENPYLIHQELGNVMTKAATVVRRNDQLAAALSTV
                     HDLYERAKKCSLSDTGQWTNQNVVFTKALLDMFPLALTILKGALTRDECRGAHYKPDF
                     AFGSLTATEPAARRQEAEEWCDRFEANNAKWLKSTIAQWTGDDVELTYEEVDTTLIPA
                     RPRLYGLVGAEDIEKVWKERAAAQAAASESDGNGSPAGKKPVAIAAH"
     gene            complement(2384..3208)
                     /locus_tag="VFV87_01830"
     CDS             complement(2384..3208)
                     /locus_tag="VFV87_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HEX5102519.1"
                     /translation="MNEVPASSFLARNEFLIRRLHSLSGLIPVGAYMVIHLLTNAAIL
                     ESPALYQRLVYGIHGLPVLWVIEWVFIFIPILFHAIIGVLMIRGSLPNSGTYKYGSNY
                     RYTLQRATGMIAFVFILVHVFHMHGWFHAEPWVKNVVEPLGGGQFKPYNAASTGALAL
                     ASIGYQIFYVVGVLSCVFHLANGIWTFGITWGIWVTPAAQRWATAAALVFGIGLGIVG
                     LGALGGFAYGVNVEEAAQKENEMYQERVKIGDIDPDDHKRAHAKPETAAVAPPTDH"
     gene            complement(3351..3494)
                     /locus_tag="VFV87_01835"
     CDS             complement(3351..3494)
                     /locus_tag="VFV87_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102520.1"
                     /translation="MLRFHLDAQVDHAIARGLEARGIDVTTTTDAGLLGEPADMVNRV
                     EFL"
BASE COUNT          633 a         1105 c         1166 g          700 t
ORIGIN      
        1 ggccgagcag gactgacgga ctttaccgtt gatcaccatc gtgcaagcgc cgcagacctc
       61 ttccaggcag ttgcagtccc aggcgaccgg ggcgaccgct ttgccatcga ctgtcttggc
      121 cctggccgcg atccgttgca ggacactgat gacgttcatc tccggctcat aatcgacgcg
      181 gtgccgctcc caatagctgg gctggccggg cgcgtcctgc cgcaaaatgc ggacctcgaa
      241 cgaagccggc ggctggaagg cggcgggggc gtggccattg ccgttgtgcg agtgatcgtg
      301 tccgtcttgg tgggccattg cggattgcgg atttcggatt gcggattgcg gattagtggg
      361 cggcgatagc gacgggcttc ttgcctgcgg gactgccgtt gccgtcggat tctgacgcag
      421 ccgcctgcgc ggcggcccgt tctttccaga ccttttcgat atcttcggcg ccgaccaggc
      481 catacagccg cgggcgcgcc ggaatgagcg tggtatcgac ctcttcgtag gtcaattcga
      541 cgtcgtcgcc ggtccactgg gcgatcgtcg atttgagcca cttggcgttg ttcgcctcga
      601 accggtcgca ccactcctcg gcttcctggc ggcgggcggc gggctcggtc gccgtcagcg
      661 agccgaaggc gaagtcgggc ttgtagtgag ccccgcggca ttcgtcgcgc gtgagcgcgc
      721 ccttgaggat cgtcaaggcg agcgggaaca tgtcgagcag ggccttggtg aagacgacat
      781 tctggttcgt ccactggccc gtgtcggaga gcgagcactt tttggcccgc tcgtaaaggt
      841 catgcacggt cgatagggcc gccgcgagct gatcgttccg gcggacgacc gtcgctgctt
      901 tggtcatcac gttgcccagc tcttgatgga tcaggtaggg gttctcgccg ccgccgctac
      961 gtttcagcaa ggcgtcgtgc cgcgactgat gctgttttct ggcctgctcg aacaggctgg
     1021 agggcaattc gctggcggct tgcttctggc ctttcagcag gctctcgaca cctggggcta
     1081 caaacaggcc gctgaagatg cacgagagaa gcgaattggc ccccaggcga ttggcgccgt
     1141 gatactgata gtcgcattcg ccgatggcgt acaggttcgg aatgctcgtg acctgattcc
     1201 gcggcgagcc ggcgaccagg ccgccatcgg cgctcttctg ataatcgacc cacagaccgc
     1261 ccattgcata atggaccgcc ggaaagatct tcatcggcgt gctgcgcgga tcgacccctt
     1321 ggaatttttc atagatctcc aggatgccgc cgagcttgcg gtcgagctcc ttccgcggga
     1381 tgtgcgtcag gtcgagatag acgcattggc ggtccttttc gaccgaaagt ccttcgttga
     1441 cgcagatatc gaagatttcg cgggtcgcga tgtcgcgcgg gacgaggttg ccgtacttcg
     1501 gatagcgctc ttccaggaag taataccgct cgctttcggg aatcgtcagc gggtcgcgcg
     1561 ggtcctgcgg cgtctttggc acccacacgc ggcccccttc gccgcgggcc gattcgctca
     1621 tcaggcgcag cttgtccgcg ccgggaatcg ccgtcggatg gacctgaatg aactccccgt
     1681 tgccgtactt ggccccgacc tggaaacaac ggctggcggc gctgccggtg caggccatcg
     1741 acatcgtcga gcggccgtaa atcagccccg gtccgccgct ggcgacgatc accgcatcag
     1801 ccggaaacgc ccggatttcc atcgagacga gatcctgccc gaccgctccg cggcagacgc
     1861 cgttgtcgtc gaggatcggc cccaggaaat cccagaattc gtacttcttg accttgcctg
     1921 ccgtttccca gcggcgaacc tgctcgtcga gggcgtaaag caattgctgg ccggtcgtcg
     1981 cacccgcgaa ggcggtgcgc ttgtaaagcg tgccgccgaa ccggcggcga tcgagcagcc
     2041 cttcgggcgt gcggttgaac gtgaccccca gacggtccat caggtcgatg atcttcggcc
     2101 cccagtaagc catctctttg accggcggct ggtgctggag gaaatcgccg ccatagaccg
     2161 tgtcgtcgaa atgcagccac tcgtcgtcgc cctgctggcg ggtcaggtcg ttgacgctgt
     2221 tgatgccgcc ctgcgcacag acgctgtgcg agcgcttgac cggaaccagg ctcatcagat
     2281 cgacatcgat gcccagctcc gagagcttca tggcggcggc gaggcccgcc agtccgccgc
     2341 cgacgatcat tatgcgtttt gcaggcatgt cagtggtcag ttgtcagtgg tcagtgggcg
     2401 gggcaaccgc ggcggtttcg ggcttggcgt gggcgcgctt gtggtcgtcg ggatcgatgt
     2461 cgccgatttt cacccgctcc tggtacatct cgttttcctt ctgggccgct tcttcgacat
     2521 tcacgccata ggcgaagccg ccgagagcgc ccaatccgac gatccccagg ccgatgccga
     2581 agacgagggc agccgcggtg gcccaccgct gcgccgcggg cgtgacccag atgccccacg
     2641 tgatgccgaa tgtccaaatg ccgttggcca ggtgaaacac gcaggagagc acgccgacaa
     2701 cgtaaaagat ctggtagccg atcgacgcca gggccagcgc cccggtcgag gcggcgttgt
     2761 agggcttgaa ctgcccgccg ccgagcggct cgacgacgtt cttcacccac ggctccgcgt
     2821 ggaaccagcc gtgcatgtga aacacgtgga ccaggatgaa cacgaaggcg atcatcccgg
     2881 tcgcccgctg cagggtgtag cgatagttgc tgccgtattt ataggtgccg ctgttgggga
     2941 gcgagccgcg gatcatcaaa acgccgatga tcgcgtggaa caggatcgga ataaaaatga
     3001 atacccactc gatgacccac agcaccggca atccgtggat gccgtacacc agccgctgat
     3061 agagggccgg gctttccagg atggcggcat tggtcagcag gtggatgacc atgtacgcgc
     3121 cgacaggaat cagccccgag agcgagtgca gccgccggat caggaactcg ttgcgagcca
     3181 ggaaagatga ggcggggacc tcgttcacgg cagacctttc tgaccgaagg gcaagtcagc
     3241 ctttagttta cggcaaaacg gggcggctgc ggagcaggcc gaatccttgc tacaagaagt
     3301 atagaaacgg gctgggggct gacaacgggg cgaaccgtcg tgatggcggg ctaaaggaac
     3361 tcgacgcggt ttaccatgtc ggcgggctcg cccaaaagcc cggcgtcggt agttgtggtc
     3421 acatcaatac ctcgagcctc cagcccgcgg gcgattgcat gatcgacttg tgcgtcgaga
     3481 tggaacctta gcacggttct actcggctgg atttagcttg ctgtcgagcg gccccgcccc
     3541 caactgattg cgaattgccg ctaccaagtc gttgtattcg cgaatctcct gcaggatctc
     3601 atcg
//
LOCUS       DASUGB010000097         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_150722, whole genome shotgun sequence.
ACCESSION   DASUGB010000097 DASUGB010000000
VERSION     DASUGB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150722"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFV87_01840"
     CDS             complement(<1..478)
                     /locus_tag="VFV87_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5102521.1"
                     /translation="MRGAIANGEKIAALRKTAGLTQDELAAGCGCDVKTIRSAERGNR
                     LDLATLRRIAARLSVEYRDIVADIASGRREANIAAAMAFLRAFDARDPDAVAACFCED
                     GAVLVFADERLPGAGEYRGRNRVRQWAVNCFAAYLAPPVEFGNYRFEAAGELVFARH"
     gene            855..>3644
                     /locus_tag="VFV87_01845"
     CDS             855..>3644
                     /locus_tag="VFV87_01845"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX5102522.1"
                     /translation="MASVVDSELRVDFITTGTTTEVGILRSDGATITLNTSYGGAWQE
                     TSFSSSAITRIVVSDVGGGTNQLLSLEGTAAILLSGGFASDGVERFETADTYDYYTGT
                     TRGPSLVTSGSGAISISAPRGIVVRGFTSLITEDGGITLAANSAGTDATIYAGTTIFG
                     TVETVGTGHISISGRGSVASVGVLIDGGVIRSTSATNAPTEGTITIQGTGGSASNSNR
                     GVVIQAHLSSLGVFSNSGNISIIGQSGNPGIQNDGVNIFGNGPGRIFAANQASISIVG
                     TAAGDGHGVVINSGVGQGAGIQIETQQGDIHIQGFGGPAGSGSNSGVSIAANERTSVT
                     SATGAIYIEGDGRLGRNGVAIGNGLGPPAVTSVSGPITVQGTAGSAAGSVAILVTNSS
                     FGTAIFASALNFIGDSFSFTGNGFISAASVSFAPLTPGTPISLGGGDILTGAKRLGLA
                     NFELNRIFAGTFIIGNAASGSVSVDALIDPTPPQNTRVSHHVEIASGGDIVFNAEFRT
                     VSPFNPFDPSAVPSRGNLLLSPGPTGSVQPKSSSTDIATRMNAGDPFNTSFTPGSDLR
                     FNIAGLTADTQYDRLTAFGGVNLAGADLALTGGFVSSTGNVFTIVSATSVTGIFNGLP
                     DGSHVIFNGRVLRVNYSATTVTLSDIGPAPSIDLAGRVFDDRDNDGLFNGNDEGVADV
                     AVQLFDESNLAVPLATVFTAADGTYLFDADLSVGSYRIVAGQPTGLLDGKEKAGSLGG
                     TVDNATDSQSIQGIVVGFDSGIAVAVGYNFAELHPSRVQGLVWEDANNNGEVDFGEQA
                     IEGASIRLTGTDDRGAAVDLIMQTDSQGLFEFLDLRPSDSSGYTLTETQPQGFVDGLD
                     VVGTVNGVVTGTSAINDVISQIAITQPGSDAINYNFAELPAVSGGLQAGRTAGIGFWQ
                     NKNGQALIKSLNGGPN"
BASE COUNT          739 a         1131 c         1088 g          686 t
ORIGIN      
        1 cgtggcgcgc gaagaccagc tcgccggccg cctcaaagcg atagttcccg aactccacgg
       61 gtggggccaa atacgccgca aagcagttta ctgcccactg tcgaacgcga ttccttccgc
      121 gatactcacc ggctccgggc agtcgctcgt cggcaaaaac caggacggcg ccgtcttcgc
      181 aaaagcaagc tgcgaccgcg tccgggtcgc gggcgtcaaa cgccctcaag aaggccattg
      241 ccgcggcgat gttggcctcc cgccggcccg atgcaatgtc ggcgacaatg tcccgatact
      301 ccacgctcag ccgtgccgcg attcgtcgca acgtcgccag gtctaagcgg ttgccacgct
      361 cggcgctgcg aatggttttc acgtcgcaac cgcaccctgc cgcgagctcg tcctgcgtga
      421 gtccggccgt tttgcgcagc gccgcgatct tttcgccgtt ggcaatcgct cctcgcatga
      481 ttcgataatt ccctcagcgg gtcgaagcgt ccagcaacgc cgtcggaccg gatggttcgt
      541 caaggaacca ggagctcacg ccggcgggga aacttcccgg aaacttccct tgcgatggcc
      601 ggttttggta cgcagcatat cctaggctcg gtatccgggc aatcgtcgcc cattggtacg
      661 aaagatttgc gcccaaggga acttccaaga aggaatgcga catgcgggtt cgatttgcga
      721 gttggtggaa acaggcaacg cggttgcggc gagcatcgag acaatcttcg cgtggccggc
      781 gatccgagcg gcctagtagg ctgaaccata ggataccgcg gctcgaagcg ctggaagacc
      841 gggcgctgtt ggccgtggcc agcgtagtgg actcggagct acgggtcgat ttcatcacca
      901 cgggcaccac gaccgaggtg ggcatactcc gtagcgatgg agccacgatc acgctaaata
      961 cctcatacgg cggcgcctgg caggaaacca gcttctcgag cagcgcgatc acgcgaatcg
     1021 tggtgagcga tgtcggcgga ggcacgaatc aattgctttc cctcgagggc acggcggcaa
     1081 tcttgctgag cggtggtttc gcttcggatg gcgtggagcg attcgagacg gccgacacct
     1141 acgactatta caccggcacg acccgtggcc cttcccttgt gacctccggc agcggagcga
     1201 tctcgatcag tgctccaagg gggattgtcg tgcgcggctt cacaagcctg attaccgaag
     1261 acggcggaat cacgcttgcg gcgaactcag caggcacgga cgccaccata tacgcgggca
     1321 caacaatctt tgggaccgtc gaaaccgtgg gaacgggcca cattagcatt tccggcagag
     1381 gatcagtcgc ctcagttgga gttctaatcg acggtggtgt gattcgttcg acctccgcta
     1441 ccaacgcgcc gacagaggga accatcacga ttcagggtac cggtggctct gcaagcaact
     1501 caaaccgcgg cgtcgtcatc caagctcact tgtcgtccct gggagtcttt tccaattcgg
     1561 ggaatatctc gatcattggt cagtccggaa accccggaat tcagaacgac ggtgtgaata
     1621 tttttggaaa cggaccagga cgcatttttg ctgccaatca agcgtcgatc tccattgtcg
     1681 gtacagccgc aggagatgga cacggcgtgg taattaattc tggcgtgggg caaggcgccg
     1741 gcattcaaat tgagacccag caaggcgaca tccacatcca aggttttggg ggtcctgcgg
     1801 gttcgggttc caactcgggc gtgagtatcg cggcgaatga gcgaacctcc gtaacttcag
     1861 caaccggggc catttacatt gagggtgatg gccgattggg gcgtaatggc gtcgcaatcg
     1921 gcaacggact tgggccaccg gcggtcactt ccgtgagtgg ccccattacc gtgcagggga
     1981 ccgctggctc cgcggcaggc agcgtggcaa tcttagttac caacagttcc ttcggaacgg
     2041 caatcttcgc cagcgcattg aatttcatcg gcgatagctt cagcttcact ggcaacggct
     2101 tcatcagtgc ggcgagtgtg agctttgcgc cactcacacc aggcacaccg ataagcctgg
     2161 gtggcggcga catcctcacc ggcgccaagc ggctgggact ggccaacttc gaactcaacc
     2221 gcatctttgc cggcaccttc atcatcggca acgcagccag cggcagtgtc agcgtggatg
     2281 cccttatcga tcccactcct ccccagaaca cccgggtgtc tcaccatgtc gagattgcca
     2341 gcggaggcga catcgtattc aatgccgagt tccgcacggt ttctcccttc aaccccttcg
     2401 atccctccgc ggtcccgagt cgcggcaact tgctcctatc accgggccca acgggcagcg
     2461 tgcaacccaa gagcagttcc acggatatcg ccacccggat gaacgccggc gacccgttta
     2521 atacttcgtt cacgcccggc agcgatctga gattcaacat cgctggattg acggccgata
     2581 cgcaatacga ccgcctcacg gcctttggcg gagtaaatct ggccggcgcc gatctagcgc
     2641 tgactggcgg attcgtctcc agcaccggta atgtgttcac catcgtctcc gcgacgagcg
     2701 tgaccggcat cttcaacgga ctgcccgatg gttcgcacgt gatcttcaac ggccgcgtac
     2761 tgcgggtgaa ctattccgcg acgaccgtaa cgttgtcgga cattggcccc gcgcccagca
     2821 tcgacctggc ggggcgcgtc ttcgacgacc gcgacaacga cggcctgttc aacggcaacg
     2881 acgagggagt tgccgacgtt gccgtacagc ttttcgacga aagcaacctc gccgtaccac
     2941 tggcgaccgt tttcaccgct gcggacggga cctacctgtt cgacgcggac ttgagtgtcg
     3001 gctcctaccg gattgtcgcc ggacagccaa caggactgct cgacggcaag gagaaggccg
     3061 gcagcctggg cgggaccgtg gataacgcga ctgatagcca atcgatccaa ggcatcgtcg
     3121 ttggcttcga ttcgggaatc gccgtcgcag taggatacaa cttcgccgag ctgcaccctt
     3181 cgcgcgtcca gggcctcgtc tgggaagacg ccaacaacaa tggcgaggtc gatttcggcg
     3241 agcaggccat tgaaggcgca tcgatccgcc tgaccggcac ggatgaccgc ggggcggccg
     3301 tcgatctcat catgcagacc gacagccagg gcctgttcga attcctcgac ctgcgcccca
     3361 gcgacagttc cggctatacg ctcaccgaaa ctcagcccca gggcttcgtc gatggcctcg
     3421 acgttgtggg cacggtgaac ggagtcgtga ccgggacgag tgcgatcaat gacgtcatca
     3481 gccaaatcgc gataacgcag ccgggttcgg acgccatcaa ctacaacttc gcggagcttc
     3541 cagctgttag cggcggtttg caagcaggtc ggaccgccgg catcggcttc tggcagaata
     3601 agaacgggca agcgctgatc aagtcgctca acggcggacc aaat
//
LOCUS       DASUGB010000098         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_984503, whole genome shotgun sequence.
ACCESSION   DASUGB010000098 DASUGB010000000
VERSION     DASUGB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984503"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1309
                     /locus_tag="VFV87_01850"
     CDS             191..1309
                     /locus_tag="VFV87_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102523.1"
                     /translation="MLLPPLGLVEIFGQKLEVQDLAVVALLVVLEGLLSIDNALVLGL
                     LAKRLPKHEQSKALWYGLAGAFVFRIAAVLLASLLLRWTFAKFLGGAYLVYIAVRHLF
                     FESKETEDDKVVLDEQGQPQIVEQRTGQPISEAEQEIEIKERVPVYMSAETRKRLGLA
                     SFWPTVVVIELTDIAFAVDSILAAIAMVGSPPPGTPPDAFHPKLWVVILGGLVGLMLM
                     RVAARLFIQLLERFPRFEVSAYLLVIVIGLKLLADWTVNSDWSFDEPKFIANRLGAWK
                     QTFDDLEHSRRQLVKSYEGWLKEHWVFGLAEGHGHHADEPIEPAPGEKPPPPDHNPLH
                     VPHLLDFHTPSRPEFIVFWGLMVICFFLGFTPKRRAAA"
     gene            1370..2827
                     /locus_tag="VFV87_01855"
     CDS             1370..2827
                     /locus_tag="VFV87_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5102524.1"
                     /translation="MHCMSHGRLLAAAWLLATTTMAAGEDASLTPAQRGYRLLTEKEY
                     LPPDFDQETFEAAWQHWPESVRSQAEKATPAERRKMAFERYGLTARPDDPTKPLQYVI
                     DDDGNWTMNCFACHGGTVPDGKGGVVVWPGAPNSRFALQTLTEETRLAKATLVKPFSR
                     MDIGSVAVPLGTSNGTTNAVMFGVVLMAFRDADLNVDTRRPIPPLTNHDMDAPPWWHF
                     QRKKQLYIDGFAEKGHRGLMQFMLVKQNGPEKFREWEDDFRDVFAYLSSIGPPKYPYP
                     IDQPLADRGRIAFSRVCAECHGTYGKAEVAPATAAPLSPRGRGAGGEGADAAADSYLE
                     KLVPIDVVGTDRVRLDALTPKGRDAYGQSWFADYGRSHNVRDPGGYVAPPFDGIWASA
                     PYFHNGSVPTLWHVLHPGQRPIVWKRKGDEYDPVRAGPAIEMFKAMPAEIKEGSTKRE
                     YFDTTAFGKSAAGHDFPDELTADEKRAVLEYLKSL"
     gene            complement(2848..>3507)
                     /locus_tag="VFV87_01860"
     CDS             complement(2848..>3507)
                     /locus_tag="VFV87_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102525.1"
                     /translation="EEVRMAIQQVEDQLAETNRVAGLPQESRPPEAIAPEPLASMLPP
                     LPDLDKPGQELPLLAFGPADVQPLGREIHGDHLFQDIAPPGGWLVGLRVTKGKPWDGA
                     IVALQPIYQVGGEYHLGQTCGSGRQALQHEQFLAKPGYAVGKIEARLGLIMNAVRIEF
                     YKVDDGRLNPADSYSTDWFGAEGGGPHAFNGEGSPLVGLAGSFQPEGEVITIQVLRKT
                     P"
BASE COUNT          649 a         1166 c         1059 g          633 t
ORIGIN      
        1 ctcatccgca ggcccgctac ttcgccctcg accgcattac gcgcgagcag gtcgaaaact
       61 acgcccgccg caagcaaatc ccactcgccg aagccgagcg ctggctcgcc cccaacctgg
      121 gctatgagcc gtagtcaccc cagctatgat gatgagttct ccgccccgcc cacctggacg
      181 acgctgactg atgctcttgc ccccgttggg acttgtcgag atcttcggcc agaagctgga
      241 agtgcaggac ctggcggtcg tggctctgtt ggtcgtgctg gaggggctgc tgtcgatcga
      301 caacgccctg gtgctgggcc tcttggccaa gcggctgccc aagcacgagc aatcgaaagc
      361 cctgtggtac ggcctggccg gggcattcgt ctttcgcatt gcggccgtgc tgctggccag
      421 cttgctgctg cggtggacgt ttgccaaatt cctgggtggg gcctatctcg tctatatcgc
      481 cgtccgccac ttattcttcg aatcgaaaga gaccgaggac gataaggtcg tgctcgacga
      541 gcagggacag ccgcaaatcg tcgagcaaag gacggggcag ccgatcagcg aagccgagca
      601 ggagattgag atcaaggaac gcgttccggt ctatatgagc gccgagacgc gcaagcggct
      661 gggcctggca agtttttggc cgaccgtggt agtcatcgaa ttgacagata tcgcgttcgc
      721 agtggattcg atcctcgcgg caatcgcgat ggtcggcagt ccacccccgg gaacgccgcc
      781 cgacgccttt cacccgaagc tgtgggtggt cattctgggc gggctggttg ggctgatgct
      841 gatgcgcgtc gcggcccgcc tgttcattca attgctcgaa cgctttccgc ggttcgaagt
      901 ttccgcctat ttgctggtga ttgtgattgg cctgaagctg ctggccgatt ggacggtcaa
      961 ttccgactgg agctttgacg agccgaagtt catcgccaac cgcctcggcg cctggaagca
     1021 gacgtttgac gacctggagc acagccgtcg gcaactcgtc aaatcgtacg aaggctggct
     1081 caaagagcac tgggtcttcg gcctggccga gggacacggc catcatgcgg acgagccaat
     1141 agagccagcc cccggggaaa aaccgccgcc gccggaccac aacccgttgc acgtcccgca
     1201 cttgctcgat ttccacaccc ccagccggcc cgagttcatc gtcttctggg gcctgatggt
     1261 aatctgcttc ttcctcggct ttacgcccaa gcgaagagca gccgcgtagc cgccgcgtcc
     1321 acgcggccgc agggattaac cgggttaact tgccatctat aatgcggtga tgcactgcat
     1381 gtcccatggt cgattgctcg ccgcggcctg gctcctggcc accaccacta tggcggcagg
     1441 ggaggacgcc tccctcacgc ccgcgcagcg cggctatcgc ctcctgaccg aaaaggagta
     1501 cctgccgccc gacttcgacc aggaaacctt cgaggcagcc tggcagcact ggcccgagtc
     1561 ggtgcgctcg caggccgaga aggcaacacc cgcggagcgg cggaaaatgg ccttcgagcg
     1621 ctatggcttg acagcccgac ccgacgatcc gacgaagccg ctgcagtatg tcatcgatga
     1681 cgacggcaac tggacgatga actgcttcgc ctgccacggc gggacggttc ctgacggcaa
     1741 agggggcgtc gtcgtctggc cgggagcgcc caactcgcgt tttgctttgc aaacgctgac
     1801 cgaagaaacg cgactggcga aggcgacact cgtcaagccg ttctcgcgga tggatattgg
     1861 cagcgtcgcc gtgccgctcg gcacgtccaa tgggaccacc aacgcggtca tgttcggcgt
     1921 cgtcttgatg gcctttcgcg atgccgacct gaacgtcgat acccgccggc cgatcccgcc
     1981 gctcaccaat cacgacatgg acgctccgcc ctggtggcat ttccagcgga aaaagcaact
     2041 ctatatcgac ggctttgcgg aaaaggggca tcgcggcctg atgcagttca tgctcgtcaa
     2101 gcagaacggc cccgagaaat tccgcgagtg ggaggacgac ttccgcgacg ttttcgccta
     2161 tctctcctcc atcgggccgc cgaagtatcc gtaccccatc gaccagcccc tcgccgaccg
     2221 cggccgcatc gcctttagtc gtgtctgcgc cgaatgccac ggcacctatg gcaaggctga
     2281 ggtcgcgcca gcgaccgcgg ctcccctctc cccgcgtggg agaggggccg ggggtgaggg
     2341 ggcagacgcg gcagcagact cgtacctcga aaaacttgtc cctatcgacg tcgtcggcac
     2401 cgaccgcgtc cgactcgatg cgctcacgcc caaaggccgc gatgcctatg gccaaagctg
     2461 gttcgccgat tacggcaggt cccacaacgt gcgcgatccg ggcggctacg tcgctcctcc
     2521 ctttgacggc atctgggcca gcgctcccta cttccacaac ggcagcgtgc cgacgctctg
     2581 gcacgtgctg catcccggcc agcgccccat cgtctggaag cggaagggtg acgaatacga
     2641 tcccgtccgc gccggtcccg ccatcgaaat gttcaaagcc atgccagccg aaatcaaaga
     2701 aggctccacg aagcgcgagt atttcgacac cacggccttt ggtaaaagcg ccgcgggcca
     2761 cgactttccc gatgagctaa ccgcggacga gaagcgggcc gtgctggagt atctgaagtc
     2821 cctgtaagcc cagcgaatcc cgcagcgtca gggcgtcttc cgcaagacct ggatcgtgat
     2881 tacttcccct tccggctgaa agctgcctgc caggccgacc aacggcgagc cttcaccgtt
     2941 gaaggcatgc ggcccgcccc cttcggcacc gaaccagtca gtggagtacg agtccgcggg
     3001 attcaaccgg ccgtcgtcca ccttgtagaa ctcgatccgc acggcattca tgatcagacc
     3061 cagccgtgcc tcgatcttgc caaccgcata tccaggtttg gccaggaact gctcgtgctg
     3121 cagggcctgc cggccgctac cgcacgtctg gccgaggtgg tattcaccgc cgacctggta
     3181 aatcggctgc aaagccacga tcgcaccatc ccaaggctta cccttggtga cgcgcaggcc
     3241 caccagccag ccgcccggcg gtgcgatgtc ctggaacagg tggtccccgt ggatctcgcg
     3301 gccgagcggc tgcacgtccg ccgggccgaa tgcgagcagc ggcaactctt gccctggctt
     3361 gtccaaatcc ggcagcggag ggagcatcga agctagcggc tcgggcgcaa tggcctcagg
     3421 cggacgagat tcctgcggca gcccggccac gcgattggtt tctgcaagct ggtcttccac
     3481 ctgttgaatc gccatccgaa cttcctc
//
LOCUS       DASUGB010000099         7479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1679139, whole genome shotgun sequence.
ACCESSION   DASUGB010000099 DASUGB010000000
VERSION     DASUGB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679139"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFV87_01865"
     CDS             complement(<1..651)
                     /locus_tag="VFV87_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102526.1"
                     /translation="MDINLKLAREHGRKEIFLSLARVPNSSRVLCGASDGRIYDLDAL
                     AEKPEFKELDGHGPFVTGVAVAGEMFVSGGYDCKLIWRKLEGDEIARTAENAHARWIR
                     KLAASPDGQRIASVADDMVCRVWDNQGGLIYELRGHDAETPNHFPSMLYTCVFSADGQ
                     KLATADRTGRVCLWNMADGEKLTQLDAPGFYTWDPKQRIHSIGGARAAAFSPDGKWL"
     gene            complement(753..2027)
                     /locus_tag="VFV87_01870"
     CDS             complement(753..2027)
                     /locus_tag="VFV87_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102527.1"
                     /translation="MNNLTRRSFLGATAVGTVALTADMTRLNVLQNKALADELRANQK
                     RVILLWLAGGASQLETFDPKPGRPTGGPFRAIPTAEPGIHISELMPKLAARIKHTAIV
                     RSLDTKNGDHGGGARLMHLGRRDEPTIKYPDLGAMVARELGRADSQVPDYVSFYTATE
                     GRGNAYSQAGFLGARYNSMFLTEQNVPPNLRRLEEIGEIDHQDRAALRDLLSARFSRG
                     RESSSLASHNEAYSRVRGLMASEKLFDISGESQQVRNLYGPTLFGEQALIARRLVEAG
                     VPFVKVSRAWWDSHGQNFETHLELATELDNVMAALIDDLEARGLLEHTLIVTLSEFGR
                     TPQINASLGRDHFGTAWSMSLTGCGVKGGSVYGETDEDGHQVKDGKVGAAEVFATIFQ
                     ALGIDHKKDYHVGSRPIPLTDPGTQPIRAVLA"
     gene            complement(2073..3710)
                     /locus_tag="VFV87_01875"
     CDS             complement(2073..3710)
                     /locus_tag="VFV87_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102528.1"
                     /translation="MRSRSLVIGLAVCCACPARGVADDLLPAETPVEQAIDHYVGAKL
                     QAAGVTAAAQAEDANVLRRTMLDLVGRIPTPAEAKAYVADPSPDKREGLVERLLASPA
                     FIRQQTAEFDALLMHGTGKDLREYLSKAFSEKRSWDQMFRDMIAGETDDSEQKGAIRF
                     VAARAKDPDKLTNDTSVLFFGVNVSCAKCHDHPLVADWTQDHYYGMFSFFSRTYDVGD
                     FLGEREYGNVNYKTVTGDSRDARLMFLSGTVLDEPPSEEPDESAKKEERKRLEELKKS
                     KQPPPPPAYSRRAQLVDAALEDDENDYFSRAIVNHLWNRFFGRGLVHPVDQMHSENPP
                     SHPELLDWLARDVRARGYDLVPLIRGIALSQTYSRSSQWDSAGESPQEKRPSDGLFAV
                     AQIRPLKPWQYGTLLKLATVSPDRFPADLSADDLDQRIQGLEGGGRGLGSQFEMPTAD
                     FQIGIDEALLFSNSERIASELLPDNKESLLGKMADCPDLRSAAETAVWSIYTRRPTEE
                     ELTALTEYLAQREDRIADARRQLVWALLTSSECRFNY"
     gene            3878..4480
                     /locus_tag="VFV87_01880"
     CDS             3878..4480
                     /locus_tag="VFV87_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5102529.1"
                     /translation="MSDAVQTASAETSSSLLHRAQSRDADAWRRLAELYGPVVYRWVR
                     QAGFQEHDAADIVQDVFQSVLTSLDRFGHQRPTDRFRDWLWTITRRRAIDFIRQRAAQ
                     PAGAGGTEAQARIQELPEQLPEANGEEAASAEAELVRRALELVRPEFAEHTWQACVQT
                     AIDGRRPADVAADLEMTVGAVYVARSRVLKRLRQELEGLV"
     gene            complement(4486..5610)
                     /locus_tag="VFV87_01885"
     CDS             complement(4486..5610)
                     /locus_tag="VFV87_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5102530.1"
                     /translation="MQIARVETFRVNYAVRGHFKFFEQPDGKPPCRPTVVVKLTADGG
                     TVGWGQCVPSPRWSYESVETCETTIRHYLAPILIGADPFAIDAIHAEMNRSIAPSFST
                     GQPIAKAGIDLALHDLVGRTTNQPTTLRLSPERNKQSEARPIALSWSINVRLLDDVGP
                     EIEQANHRGYASFNVKVGPDLALDLELCRLVKHLAPSAVVWADANGGYDEESAAAAAK
                     DLAKLGFAAIEQPLPANRLGGYQRLVQQRALPIVMDEGIVSCVELEEFHRLGLLDGVA
                     MKVARCGGLTEAGRIMEFARNEGLMILGSGLTDPDLSLAASLILFSAYDLQIPTALNG
                     PQYLTTSILRKPLVVENGAIAPPSDPGLGVEVDEAALLSA"
     gene            complement(5681..6742)
                     /locus_tag="VFV87_01890"
     CDS             complement(5681..6742)
                     /locus_tag="VFV87_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5102531.1"
                     /translation="MNRNSQSRDQWSRFTRRQWIAATGVAVVAAAGAPGASAAAQCAS
                     ANEADEPALIAITLDLEMSRNFPTRETTHWDFEKGNLNAETKAYAIEAARRVKARGGR
                     LHFFVVGRVLEQENVEWLKQLAADGHPLGNHTYDHVNVKATKPEDIQFRFQRSPWLIE
                     GQKPADVIRENIRLTSVAMKSRLNVAPAGFRTPGGFGPGLADREDVQRMLLDQGFTWC
                     SGKYPGHAINAPKEVESPSEPADEIIASIVAAQANAQPFIYPSGLVEVPMSPISDIGA
                     FRTGRWKLEWFLNAIRAGVTWAIDNRACYDFLGHPSCLYVTDPDFQTIDLICDLVEKA
                     GPKAKLADLNALSQRANRS"
     gene            complement(6732..>7479)
                     /locus_tag="VFV87_01895"
     CDS             complement(6732..>7479)
                     /locus_tag="VFV87_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5102532.1"
                     /translation="VFFGRQGWRTPLPAGVADFCTLEKNKLAGRHTREVINPLLVRLR
                     GQAAFVMYSLPGKEDAIRKKLYRSVHSSPTDEDRKAGGKELDAKLNSFVYRGESLPEK
                     ITLSADKPVIDYFQQFPGLDAGPRYNSAGFWDLPVPVTKDIEYHAQDIVIYDAEGYSV
                     LKEFLQQHGVKHVLLVGYATDMCFCKTTAGYENLSKDFNVFLVGDATLATFPANNTPK
                     YATNAHISFAALNQLVTQCSWIKLDVDVEP"
BASE COUNT         1312 a         2365 c         2378 g         1424 t
ORIGIN      
        1 gagccatttg ccgtccggcg agaacgccgc cgcccgcgcg ccgccgatcg agtgaatccg
       61 ctgcttggga tcccaggtgt aaaagcccgg cgcgtcgagc tgcgttagct tctcgccgtc
      121 ggccatgttc cacagacaga cgcggccggt cctgtccgcc gtcgccagct tctgcccgtc
      181 agcagagaag acgcaggtgt agagcatcga cgggaagtgg ttcggcgtct ctgcgtcgtg
      241 ccctcggagc tcgtaaatca agccgccctg gttatcccac acgcggcaga ccatgtcgtc
      301 ggcgacgctc gcgatccgct gcccatcggg cgaagcggcc agcttgcgaa tccagcgggc
      361 gtgggcattc tccgccgttc tagcgatttc gtcgccctcc agcttccgcc agatcagctt
      421 gcagtcgtac ccgccgctga cgaacatctc gcccgccacg gcgacgccgg tgacgaacgg
      481 cccgtggcca tccaactcct tgaactccgg cttctcggcc agtgcgtcca ggtcgtagat
      541 cctgccatcc gacgcgccac aaaggacgcg gctggaattc ggcacgcggg ccaggctcaa
      601 gaaaatttcc ttgcggccgt gctcgcgggc gagtttgagg ttgatgtcca tcgccgtggg
      661 gcaggcatcc tgcctgccgt tatcgggtga gaattgagat gaggttgtgc gaatggcaaa
      721 cggatatttg gcaggctgga agcatgcccc acttacgcca gcaccgcccg aatcggctgc
      781 gtccccggat cggtcagcgg aatgggccgg ctgccgacgt gatagtcctt cttatggtcg
      841 attcccaggg cctggaagat cgtcgcaaag acctcagccg cgccgacttt gccatccttg
      901 acctggtggc cgtcttcatc ggtctcgcca tagaccgagc cgcctttcac gccacagccg
      961 gtgagcgaca tgctccaggc ggtgccgaaa tgatcgcggc cgaggctggc gttgatttgc
     1021 ggggtgcggc cgaactcgga gagtgtgacg atcagcgtgt gctccaacag accgcgggct
     1081 tccagatcgt cgatcagggc ggccatgacg ttatcgagct cggtggccag ttccaggtgc
     1141 gtctcaaagt tctggccatg gctgtcccac caggcccgcg aaaccttgac aaagggcacg
     1201 ccggcttcga ccaggcggcg ggcaatcagg gcctgctcgc cgaagagcgt cgggccatac
     1261 agattgcgga cttgctgcga ttcgcccgaa atgtcgaaca gcttctcgct ggccatcagg
     1321 ccgcggacgc gggaataggc ctcattgtga ctggcgagcg acgatgactc acggccgcgg
     1381 gaaaatcgcg ctgagagcaa atcgcgcaac gcagcccggt cttgatggtc gatctcgccg
     1441 atttcctcca gccgccgcag gttgggcggc acgttctgct cggtgaggaa catcgagttg
     1501 tagcgagcgc cgaggaagcc cgcctggctg taggcgttgc cgcggccttc ggtggccgtg
     1561 tagaagctga cgtagtcggg cacctggcta tcggcacggc ccagctcgcg ggcgaccatc
     1621 gcgccgaggt cgggatactt gatcgtcggc tcgtcgcggc ggccgaggtg catgaggcga
     1681 gcgccgccgc cgtggtcgcc gtttttggtg tcgagcgagc ggacgatagc ggtgtgcttg
     1741 atccgagccg ccagcttggg cattagctcg ctgatgtgga tgccgggctc ggccgtgggg
     1801 attgcgcgga acggcccgcc ggtcggccgg ccgggcttcg ggtcgaacgt ttcgagctgg
     1861 ctcgcgccgc cggccagcca gagcaggatc acgcgcttct ggttcgccct cagctcatcg
     1921 gcgagcgcct tgttttgcag cacgttcagc cgcgtcatgt ccgccgtgag agcgacagtg
     1981 ccgacggcgg tcgcgccgag gaaggagcgg cgggtgaggt tgttcatgac atgtcctcca
     2041 tcatttccgc gaccactaat cgcggctttg gatcaataat tgaatcggca ctcgctgctg
     2101 gtgagcaggg cccacacgag ctggcggcgg gcatcggcaa tacggtcttc gcgctgggcg
     2161 aggtattcgg tcagggcggt caattcttct tccgtcggtc ggcgagtgta aatggaccaa
     2221 accgccgtct cggcggccga tcggaggtcg gggcagtcgg ccattttgcc gaggagcgat
     2281 tccttattgt caggcagaag ttcgctggcg attcgctcgc tgttgctgaa gagcagggcc
     2341 tcatcgatgc caatctgaaa atcagccgta ggcatttcaa actggctccc cagaccgcgg
     2401 ccgcccccct ccaggccctg gatccgctgg tcgagatcgt ctgctgaaag atcagccggg
     2461 aaacggtctg ggctgaccgt cgccagcttc agcagcgtgc catattgcca cggcttgagc
     2521 gggcgaatct gagccaccgc gaacagcccg tcgctcggcc gcttctcctg cggcgactcg
     2581 ccggcggaat cccactgact gctgcggctg taggtctggc tgagggcaat gccgcgaatc
     2641 agcggcacaa gatcatagcc gcgggcgcgg acatcgcggg ccagccagtc gagaagctcc
     2701 gggtggctcg gcggattctc cgagtgcatc tgatcgaccg gatggacgag gccccggcca
     2761 aaaaagcggt tccagaggtg gttgacaatc gcccgtgaga aatagtcgtt ctcgtcatcc
     2821 tcaagggctg catcgacaag ctgcgctcgc cggctataag cgggcggtgg cggcggctgc
     2881 ttgctcttct tcagctcttc gaggcgcttg cgctcctctt tcttggccga ctcatccggc
     2941 tcctcgctcg gtggttcatc aaggaccgtg ccgctgagga acatcagcct ggcatcccgc
     3001 gagtcgccgg tgacggtctt gtagttcacg ttgccgtatt cccgctcacc cagaaaatcg
     3061 cccacatcat atgtgcggct gaagaacgag aacatgccgt agtaatggtc ttgcgtccaa
     3121 tcggcgacga gcggatggtc gtggcacttg gcacaactca cattcacgcc gaagaacagc
     3181 acgctcgtgt cgttggtcag cttgtcgggg tccttggccc gcgcggcgac gaagcggatc
     3241 gcccccttct gttcgctatc gtccgtctcg cccgcgatca tgtcgcggaa catctggtcc
     3301 cacgaacgct tctcgctgaa agccttgctc agatactcgc gcaggtcctt gccggtgcca
     3361 tgcatgagga gcgcatcgaa ctccgccgtt tgttgccgga tgaaggcggg cgaggccagc
     3421 agccgttcga cgagcccctc gcgcttgtcc ggcgacggat cagcaacata cgccttggcc
     3481 tccgccggcg tcggaatccg gccgaccagg tcgagcatcg tccgccgcag cacgttcgca
     3541 tcctcggcct gggccgcggc agtgacgccg gccgcttgca gtttcgcgcc cacatagtgg
     3601 tcaatcgcct gctcgaccgg cgtttccgcc ggcagcagat cgtcggccac tccgcgcgcg
     3661 gggcaggcgc agcaaacggc caaaccaatc actagcgaac gactgcgcat gcggctgttt
     3721 cctcgcaggc aggcaaaggg caggatgttc aggcgggacc gccaacttcc atcagcggcg
     3781 atttatatgg tagcggaatt tcgcgaccga tgccaacaag aatcctctgc caattgtgcg
     3841 acccgcgcga ttatcatggg cccagcgagg atcggcgatg agcgacgcgg tgcagacggc
     3901 cagtgcagaa acctcttcca gtttgctgca tcgcgcccag agccgggacg cggatgcctg
     3961 gcggcggttg gcggagttgt acggcccggt cgtctatcgc tgggttcgcc aggcgggatt
     4021 tcaggagcac gacgcagccg acattgttca ggatgtgttt caatcggtgc ttaccagcct
     4081 ggacagattt gggcaccagc ggccgaccga ccgctttcga gactggctgt ggacgatcac
     4141 ccggcggcgg gcaatcgact tcatccgcca gcgggccgca cagcccgccg gcgcgggcgg
     4201 aaccgaggcg caggcgcgaa tccaggaatt gcccgagcaa ctgcccgagg caaacggcga
     4261 ggaggccgcg agcgccgaag ctgagctggt gcgccgagcg ctggagctcg tgcgaccgga
     4321 gttcgccgag cacacctggc aagcctgcgt gcagaccgcg atcgacggcc gccggcccgc
     4381 cgacgtcgct gccgatctcg aaatgaccgt cggcgcggtt tatgtcgccc gctcgcgggt
     4441 gcttaaacgc ctgcggcagg agttggaggg gttggtgtaa gcgtcctaag ctgacagcaa
     4501 agcagcctcg tccacttcga cacccaagcc agggtcgctg ggaggcgcga tcgcgccatt
     4561 ctccacgacc aacggcttgc gcaggatgct cgtcgtcaga tactgcgggc cgttgagcgc
     4621 cgtggggatt tgcaggtcgt aagccgagaa cagaatgagc gacgcggcca gcgagaggtc
     4681 ggggtcggtg agcccgctac cgaggatcat gagtccctcg tttcgggcaa actccatgat
     4741 ccggcccgct tcagtcaggc cgccgcaccg ggcaactttc atcgctacgc catcgagtag
     4801 ccccaacctg tggaactcct ccagttcgac gcacgacacg atgccctcat ccatcacaat
     4861 cggcagagcc ctttgttgaa cgagccgttg atagccgcct agccgattcg cgggtaacgg
     4921 ctgttcgatc gcagcgaacc ccagtttggc caagtccttg gctgccgcag ccgcggattc
     4981 ctcatcatag ccgccgttgg cgtctgccca gacaaccgcg cttggcgcaa ggtgcttcac
     5041 aagccggcac aattccagat ccagcgccag gtctggaccg accttgacat tgaagctcgc
     5101 gtagccgcgg tgatttgctt gttcaatctc tggaccaaca tcgtcgagca agcgaacgtt
     5161 gatcgaccag ctcagcgcaa ttggccgcgc ttcgctctgc ttgtttctct cgggagagag
     5221 gcggagcgta gtgggttgat tcgttgtgcg acccaccaga tcgtgcagcg ccaggtcaat
     5281 ccccgccttt gcaattggct ggccggtcga aaacgacggc gcgatggacc ggttcatttc
     5341 cgcgtgaatc gcgtcaatcg cgaaaggatc tgcgccgatc aagatgggcg ccaggtaatg
     5401 gcggatcgtt gtctcgcacg tttcgaccga ttcatagctc caccgcggcg aagggacgca
     5461 ctggccccag ccgacggtgc cgccatcagc cgtcaatttc accacgaccg tcggccggca
     5521 agggggcttg ccgtcgggct gctcgaagaa cttgaagtgg ccgcggacgg cgtagttgac
     5581 gcgaaaggtc tcgacgcggg cgatctgcat atcgtgaggc aggcttccag cctgctaaaa
     5641 aaaccggaaa cgatttttgg caggcaagat gcctgcccca ctaggaccga ttcgcccgct
     5701 ggctcaaagc gttcaggtcg gccagttttg cctttggtcc agctttctcg accagatcgc
     5761 agatcaggtc gatggtttgg aaatcgggat cggtcacgta gaggcacgag ggatggccga
     5821 ggaaatcgta gcaggcgcgg ttgtcgatgg cccaggtgac gccggcgcgg atcgcgttca
     5881 ggaaccactc cagcttccac cgccccgtgc ggaacgcgcc gatgtcgctg atcgggctca
     5941 tcggcacttc cacgaggccg ctgggataga tgaacggctg ggcattggcc tgagcggcga
     6001 cgatgctggc gatgatttcg tccgctggct ccgacggtga ttcgacttcc ttcggcgcgt
     6061 tgatcgcgtg ccccgggtac ttgccgctgc accacgtgaa gccttggtcc agcagcatcc
     6121 gctgaacgtc ctcgcgatcg gccagtcccg gcccaaagcc gccgggcgta cgaaacccgg
     6181 ccggcgcgac attcagccgc gatttcatcg ccacactcgt caggcggatg ttctcgcgga
     6241 tcacgtccgc cggcttttgg ccttcgatca gccacggcga gcgctggaag cggaattgga
     6301 tgtcctcggg ttttgtggcc ttgacgttga cgtggtcgta cgtgtggttg ccaagcggat
     6361 ggccatccgc ggcaagctgc ttgagccact cgacgttctc ctgctccagc acacggccga
     6421 cgacgaaaaa atgcagcctg ccgccgcggg ctttcacgcg ccgagccgcc tcgatggcat
     6481 aggccttcgt ttcggcattg agattcccct tctcgaaatc ccaatgcgtc gtctcccgcg
     6541 tgggaaagtt gcggctcatc tccaggtcga gcgtgatggc gatcagagcg ggctcgtccg
     6601 cttcattcgc acttgcacat tgcgccgcgg cgcttgcgcc gggcgcaccc gcagcagcga
     6661 caactgccac accggttgcg gcaatccatt ggcgacgcgt aaaccgcgac cactggtcgc
     6721 ggctttgcga attacggttc aacgtcaaca tccaacttga tccacgaaca ttgcgttacg
     6781 agctgattca gggccgcgaa agagatgtgg gcgttggtcg cgtatttcgg cgtgttgttg
     6841 gccgggaacg ttgccagcgt ggcgtcgccg accaggaaca cgttgaaatc cttcgacagg
     6901 ttctcgtagc ccgccgtcgt cttgcagaag cacatatcgg tcgcgtagcc gacgagcagg
     6961 acgtgcttga cgccgtgctg ctgcaggaat tccttcagca cgctgtatcc ctcggcgtcg
     7021 tagatcacga tgtcttgcgc gtgatactcg atgtccttcg tcaccgggac cggaagatcc
     7081 cagaagccgg cactgttgta tcgcgggccg gcatccaggc cgggaaactg ctggaaatag
     7141 tcaatcaccg gcttgtcggc cgagagtgtg atcttttccg gcagcgactc gcccctatag
     7201 acgaagctgt tcagtttggc gtcgagttcc ttgcccccgg ccttgcggtc ctcgtcagta
     7261 ggtgatgagt gcacggaccg atagagcttc ttgcgaatcg cgtcctcctt gcctggcagc
     7321 gagtacatca cgaacgccgc ttgcccccgc agcctcacca gcagcggatt gatcacctcg
     7381 cgcgtatgcc ggccggccag cttgttcttc tccagcgtgc agaaatcggc cacgcccgcc
     7441 ggcagcggcg tccgccagcc ctgccttccg aagaacacg
//
LOCUS       DASUGB010000100         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_151403, whole genome shotgun sequence.
ACCESSION   DASUGB010000100 DASUGB010000000
VERSION     DASUGB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151403"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            597..1598
                     /locus_tag="VFV87_01900"
     CDS             597..1598
                     /locus_tag="VFV87_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102533.1"
                     /translation="MWTRIVQISPALAALLYSGVLMAQDSVSTGSGNLLHQVKWLRLE
                     VIGGRIVAHSDRCSQNRYSVESSASGHSRQTLNLETQAATLVVRYENIDAQTRLLLEL
                     NERGQLSIERTGGPDSQPDVSYFQAPCGKVKLTVGGTKPRTIIAENLWQLLLAERDLC
                     RERFLPLLDALRPNWQLEEQLDDVEATLMKQAGANVLAQRRQWQIWVDQLATGDYSQR
                     QAADRALRQVGQPVLAFLLGLDATSLDGEQRRRVRTMLRDLPDGSADSPLRVADWLAA
                     DKRVWLALMARGEVDQRIAAAEHLSKLCRRPLVFDPQATAEARQAQLAELSAKLVEN"
     gene            1840..2382
                     /locus_tag="VFV87_01905"
     CDS             1840..2382
                     /locus_tag="VFV87_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5102534.1"
                     /translation="MNQPPIDWQAALGEHDRWLRTVVFARLREPHAVEEVMQEVALAA
                     VKQSAPISDRGKVAPWLYRLAVRQALLYRRKCGRRRRLVGRFAEQVQAGDRGPPPALD
                     PLGWLLAEERQALVRRALSRLSRRDAEILLLKYTEEWSYHQIAAHLGIGHAAVEARLH
                     RARARMRQELAALDVIEAVR"
     gene            2419..3168
                     /locus_tag="VFV87_01910"
     CDS             2419..3168
                     /locus_tag="VFV87_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102535.1"
                     /translation="MNDKIAGTSRLPDEDVRFDRLVDGELSPDESRALLASLDDEPGG
                     WRRCALAFLESHALTGELRGVRRSLHWSDDGGSSPQETPQVALVRQSPRNLRQFELRT
                     LLAMAASFIVALGLGIVAPKWFSTGTSQPPSIADVPSDSPGGGVVASPHLRRESFKPI
                     GNVQLVVDGPSGESTNMGNLPIYEHSEPLEEVLSNERPALPLEVLRSLNELGHQVERS
                     VEYVPVQLQDGRRGYVPVEGYQITPAGRRSY"
BASE COUNT          595 a          941 c         1078 g          580 t
ORIGIN      
        1 tctcgccgtc gtcccgctcc tgcaggcgga agatcgcccc gccggcgggc tgaccgacgc
       61 gatgaagaag agcaagccgc tctgcgtccg ccagcacgcg aatgctgctc gccacggctt
      121 tcatcgtggc taggggctgg gcacgttctt cgtcggtggg cttgtctggc tgctgagccc
      181 agagcgcgga aacaatcccg aggagacagc caaggagcgc gcatcgaccc aattgcgaag
      241 ctgcgaacat aggacctcca aaacagacat tgtgtctata tcggctaacg tgcagattag
      301 cggcgcggcg ggcgatttgc aaaccgggga gacatcggta gacattggtg ggcggcggag
      361 cagatttgct cttggaggac ccatcgacag gccgatatat atgagaaggg tcgccccgcg
      421 aattgcgagg gagaaccgac agcgggaaat cgaaccagca gcggctgaaa acaagtcggc
      481 actggaacaa gcggctttga caggcgcgcg aatcgaccaa tcgcgcggtc caggcccagc
      541 cccgcctgaa aatcctctcc aatttgcttc ggcacggaag ccatggggta atagccatgt
      601 ggacgaggat cgttcagatt tcgcctgcgc ttgccgccct tctctactcg ggcgtgctca
      661 tggcccagga ttcagtttcg accggcagcg gaaatctgtt gcaccaggtc aaatggctgc
      721 ggctggaggt catcggcggg cggattgtcg cccacagcga ccgctgcagc cagaatcgct
      781 attccgtcga gtcatctgcc tctggccact cgcgccagac gctcaatctg gagacccagg
      841 ctgcaacact cgtcgtccgc tacgaaaaca tcgatgccca gacgcggctt ctgctggaat
      901 tgaacgagcg cgggcagtta tcaatcgaaa ggaccggcgg gccagattcg cagcccgatg
      961 tttcgtattt ccaggcgccc tgcggcaaag tgaagctgac ggttggcggc acaaagccgc
     1021 gaacgatcat cgccgaaaac ttgtggcagc tcctgctcgc tgagcgcgac ttgtgccgcg
     1081 agcgcttcct gccactgctc gacgcgctgc gtccaaactg gcaactcgag gagcaactcg
     1141 acgacgtgga agcaacgctg atgaagcagg ccggtgcaaa tgtgctggcg cagcggcgtc
     1201 agtggcagat ttgggtcgat caattggcga ccggcgatta ttcgcagcgg caagcggcgg
     1261 atcgcgccct gcgacaggtc ggtcagccag tgctggcgtt tctcctgggc ctggacgcaa
     1321 cctcgctgga tggagagcag cgtcgccgcg ttcgtacgat gctgcgcgat cttccggatg
     1381 ggagcgccga ttcgccgctg cgcgtggccg actggctggc ggctgacaag cgagtctggc
     1441 tggcgctgat ggcccgcggc gaagttgatc agcgaattgc cgccgccgag catctgagca
     1501 agctttgccg ccggccattg gtctttgacc cccaagcgac ggcggaggcc cgccaggccc
     1561 agcttgccga gctgtctgcc aagctggttg agaactaggg aatcttcttc ctcattgatc
     1621 ctctgaaatg gctgagtttc tgagaagcaa tgggtaatta tctcccattg ctggctcgct
     1681 ttcgcgtgtt tcgattgccc gtttctgctg ccatcgcctc ggcatcgcga aagattggcc
     1741 gtccgctgga aacatagaat cacagagagt ggatggcacg tttggcgagc agctgatcgt
     1801 tgccagccgc cctggagccc cggccagcgg gcgtgcggca tgaatcagcc ccctatcgac
     1861 tggcaggcag cgctcggcga gcacgaccgc tggctgagga ccgtggtctt tgcgcgcttg
     1921 cgcgagccac atgccgtcga ggaggtgatg caggaagtcg ccctggccgc ggtaaaacag
     1981 tcggcgccga tttccgaccg gggaaaggtc gcaccgtggc tgtaccggct ggcggtccgg
     2041 caggccctgc tgtaccggcg caaatgcggc cggcggagac gactggtggg ccggttcgcg
     2101 gagcaggtgc aggcgggcga ccgcgggccg ccgccggccc tcgacccgct cgggtggctc
     2161 ctggcggagg agcggcaggc acttgtccgg cgggccttaa gccgcctgtc acggcgcgat
     2221 gccgagattc tgctgctcaa gtacacggaa gagtggagtt atcaccagat cgccgcccac
     2281 ctgggaattg gccacgccgc cgtggaggcc cgactgcacc gggcgcgggc gcgaatgcgg
     2341 caggagttgg cggcgctgga tgtgattgag gctgtgcggt gaggacagag gccagaagtc
     2401 agaggatagg atgctgagat gaatgacaaa attgcgggca caagccgact gccagatgag
     2461 gatgttcgtt tcgatcgcct ggtcgatggc gaactctcgc ccgatgaatc tcgcgcgcta
     2521 ttggcgtccc tcgacgacga gccgggcggc tggcggcggt gcgcgttggc gtttttggaa
     2581 tcgcacgcgc taaccggcga gctgcgcggc gtgcggcggt cgcttcattg gagcgacgat
     2641 ggtggtagtt cgccgcagga gacgccgcaa gtcgcgttgg tcaggcagtc gcctcgcaac
     2701 ctgaggcaat ttgagcttcg gacgctcctg gctatggccg cgagctttat cgtcgcactc
     2761 gggctgggaa ttgtcgcgcc gaagtggttc tccactggca cttcccagcc gccatcgatc
     2821 gccgatgtgc cgagcgactc gccagggggc ggcgttgtcg cgagtcctca ccttcgccgc
     2881 gagtcgttca agcccatcgg caacgtgcaa ctggtcgttg atggcccgtc tggcgagagc
     2941 accaacatgg gcaatctgcc gatctacgaa cattccgaac ccttggaaga agtgctgtcc
     3001 aacgaacgac cggccttgcc gctggaggta ttgcggtcgc tgaacgagtt gggccatcag
     3061 gtcgagcgga gcgtggagta tgttcccgtc caactgcagg acggccggcg gggatatgtt
     3121 cccgtcgagg gatatcagat tacgccggcg ggaaggcggt cgtattgagg acaggggaca
     3181 ggggacaggg gaca
//
LOCUS       DASUGB010000101         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_290036, whole genome shotgun sequence.
ACCESSION   DASUGB010000101 DASUGB010000000
VERSION     DASUGB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290036"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..>2091)
                     /locus_tag="VFV87_01915"
     CDS             complement(439..>2091)
                     /locus_tag="VFV87_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5102536.1"
                     /translation="RGAAEIEIPAMPFADITQAAGIEFVHESGASGDKLLPETMGSGC
                     AFLDYDNDGDQDLLFVNARRWPWDERPAGQAAMPALYQNDGSGKFQNVTANAGLDISF
                     YGQGVACGDYDGDGRVDLYFTAVGKGKLLRNTGERFEDVTEAAGVGGNEGDWGTSCGW
                     FDYDRDGDLDLFVCNYLAWSRQADVSQDFQLIGGGRAYGRPQNFGGTFCTLHQNQGDG
                     TFRDVSQAAGIQIQNPDTKVPAGKSLGLAFADFDDDGFLDVVVANDTVGNFLLHNQRD
                     GTFEEIGSPAGVAYDTSGMARGAMGIDVAHFRNNREQGIAIGNFSNEMTALYVNQGDA
                     MHFRDDAVSNGLGPATRLELTFGLFFFDADLDGRLDLFSANGHLEEDIHRVQATQHYA
                     QPPHFFWNCGPERKPEFEPVPAEKCGPDLLVPMVGRGAACADIDGDGDLDIAVTATGG
                     PARLLRNDQQLGHHWLRVKLEGKAPNTGAIGAAIELHQGNSIQRRLASPTRSYLSQCE
                     LPLTFGLGADAQPTKLIVRWPDGKITERTDFAIDRQIILQQP"
BASE COUNT          337 a          648 c          713 g          393 t
ORIGIN      
        1 acacctcgcc atatcttcct gttcctgtcc acgctgcaag gaatcggcat tccccgccgc
       61 gctcttgctg gcgatcatga cgcagaagcc ctcattgcct tgctgcagcg tttgaccggc
      121 gcgaaagtgg ttcgcggcta ggggcgcagc gggcaaaggg gcgttccaga atggaccaga
      181 cgtgtccttt tcgtttgggt gggtgaaaaa aacttagggc gcggggcggg agcgcaaggg
      241 gttggtgggc aggagtttat gcgcgggcgc ttcgccgtaa gtccgcgcgg agcaagaaaa
      301 tcggcggaat gtgcttggag cgcagggatg cggtttgtaa actcccgagc cgtagtgaac
      361 atctattcac aattcaagta gggtggggcg tgtccaccgc atgttccgtc ggtgggcaca
      421 gcccacccta cgcccccact agggttgctg caggatgatc tggcggtcga tggcgaagtc
      481 ggttcgctcg gtgatcttgc cgtccggcca gcggacgatg agcttcgtcg gctgggcgtc
      541 ggcgcccagg ccgaacgtga ggggtagctc gcactgcgaa agatagctgc gggtggggct
      601 cgccagccgt cgctggatgg aattgccctg atgcagctcg atggcggcgc cgatcgcgcc
      661 ggtgttcggc gcctttcctt ccagcttgac gcggagccag tggtggccga gctgctggtc
      721 gttgcgcagc aggcgcgccg gcccgccggt cgcggtcacc gcgatgtcga ggtcgccgtc
      781 gccgtcgata tcggcacagg ccgcgccgcg gccgaccatc ggcacgagca ggtccgggcc
      841 gcacttctct gccggcacgg gctcgaactc cggctttcgc tccggcccgc agttccagaa
      901 gaaatgcggc ggctgagcat agtgctgcgt ggcctgcacg cggtggatat cctcctccag
      961 gtggccgttg gcggagaaca ggtccagccg cccgtcgaga tcggcgtcga agaagaacag
     1021 gccgaacgtc agctccaggc gcgtcgccgg cccgaggcca ttggacacgg cgtcgtcgcg
     1081 gaagtgcatg gcatcgccct ggttgacata gagagcggtc atttcgttcg agaaattgcc
     1141 gatcgcgatg ccttgctcgc ggttgttgcg aaaatgggcg acgtcgattc ccatcgcgcc
     1201 gcgggccatg ccgctggtgt cgtaggcgac gcccgccggg ctgccgatct cttcgaacgt
     1261 gccgtcccgc tggttgtgga gcaggaagtt gccgaccgtg tcgttggcta cgacgacatc
     1321 caggaagccg tcgtcgtcga aatcggcaaa ggccagcccc agcgacttgc cagcgggaac
     1381 cttggtatcg ggattctgaa tctgaatgcc ggcggcttgc gagacgtcgc ggaatgtgcc
     1441 gtctccctgg ttctgatgga gcgtgcaaaa cgtgccgccg aagttttgcg ggcggccata
     1501 ggcccggccg ccgccgataa gctggaaatc ctgcgataca tcggcttggc gcgaccaggc
     1561 gaggtagttg cagacgaaca gatcgagatc gccgtcgcgg tcgtagtcga accagccgca
     1621 gcttgtgccc caatcgccct cgtttccgcc gacgccggcc gcctcggtca cgtcctcgaa
     1681 ccgctcgccg gtgttccgca gcaactttcc cttgccgacc gcagtgaagt agagatcgac
     1741 gcggccgtcg ccgtcatagt cgccgcaggc cacgccctgg ccgtagaacg agatgtccag
     1801 gcccgcgttt gccgttacgt tctggaactt gccgctgccg tcgttctgat agagggccgg
     1861 catggctgcc tggcccgcgg ggcgctcgtc ccagggccag cgccgcgcgt tgacgaagag
     1921 caggtcctgg tcgccgtcgt tgtcgtaatc caagaacgca cagccgctcc ccatcgtttc
     1981 cggcaagagc ttgtcgccgg aggcgccgct ttcgtggacg aattcgatgc cggctgcctg
     2041 cgtgatgtcg gcaaacggca tcgcggggat ttcgatctcg gctgcgccgc g
//
LOCUS       DASUGB010000102         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_846355, whole genome shotgun sequence.
ACCESSION   DASUGB010000102 DASUGB010000000
VERSION     DASUGB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846355"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFV87_01920"
     CDS             <1..1140
                     /locus_tag="VFV87_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102537.1"
                     /translation="DVQNVPPITGYVLTSVKENPLVEVLMREQGADPQNSTILAGWRY
                     GSGKTAAFTTDAGRRWAGQWTSWENYDKFFSQMIRWAMRPVNEEGKFNIASDVKDGKV
                     RVVVTALDKEDEFLNFLNMTGAGSGPELENIDIQFRQEAPGRYVAEFPADKAGSYLMA
                     INTGQANAPPLLTGITVPYSAEFRERESNQALLETLASFKPKGGEKGELVQGDLEPGM
                     VDRMVQSVDTFRRTLAKAISSEDFWPVFLLIAACIFLIDVFIRRVTVHFYWIWPALAF
                     AYNRVRGHQQEAAPDERLARLRNRKAAISSQIDERRAAARFEPQPDGFSEPRDYEQVM
                     QDASAGGPPPDQPRPQAPTQASGPQTEQDTYTERLLAAKKKAKKE"
     gene            1150..1590
                     /locus_tag="VFV87_01925"
     CDS             1150..1590
                     /locus_tag="VFV87_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5102538.1"
                     /translation="MRIYLDNCCLNRPFDDCTQVRIALEAEAVSAILRLCESGAWTLI
                     GSDALDFEAGQAPLGPRKAWLEEVLLGAGEFVPMSADIVARATELETRGLKNIDAIHV
                     ASAEAVQADIFCTCDDRLLRIARKQADLRARVASPLEIIQELEP"
     gene            1587..1814
                     /locus_tag="VFV87_01930"
     CDS             1587..1814
                     /locus_tag="VFV87_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102539.1"
                     /translation="MIQRTQSLSEMSERAVRMLVRELGVVETLRFLNQFRVGNGDYTK
                     DRDALFAQQSVPELAEQIKQWRAANPRPTSG"
     gene            1847..2269
                     /locus_tag="VFV87_01935"
     CDS             1847..2269
                     /locus_tag="VFV87_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102540.1"
                     /translation="MPVDLETVDLLVTAGKYTEAVQLLRSIADDPAAPSQARVDAFQF
                     LGTMTKVDPSAMGEDFALPYFQRALQLKPDHLWALQGIVATFGDHAPDHQDKAALLFA
                     AERLLARANELPQAVVSEVRQRLERFHAGPPGADAAPH"
BASE COUNT          524 a          712 c          747 g          483 t
ORIGIN      
        1 gatgttcaga acgttccgcc gattaccggc tatgtgctga cgagcgtcaa ggagaacccg
       61 ctcgtcgaag tgctgatgcg cgagcaaggg gccgaccccc agaacagcac gattctcgcc
      121 ggctggcggt atggatcggg aaaaacggcc gctttcacga ccgatgccgg ccgccgctgg
      181 gccgggcaat ggacgagctg ggagaactac gacaagttct tcagccagat gatccgctgg
      241 gcgatgcggc cggtaaacga ggagggcaaa ttcaacatcg ccagcgacgt gaaggacggc
      301 aaagtccgcg ttgtcgtgac ggctctcgac aaggaggacg agttcctcaa tttcctgaac
      361 atgacgggcg ccggcagcgg gccggagttg gaaaacatcg acatccagtt ccgccaggaa
      421 gccccgggcc gctacgtcgc cgaattccct gccgacaagg cgggcagcta cctgatggcg
      481 atcaacaccg gccaggccaa cgccccgccg ctcctgacgg gcattaccgt gccgtattcg
      541 gctgaatttc gcgagcgaga gtcgaaccag gctcttttag agaccttggc cagcttcaag
      601 cccaaaggag gcgagaaagg cgagctggtc cagggggacc tggagcccgg catggtcgac
      661 cggatggtgc aatcggtcga tacttttcgc cgcacgctgg ccaaggcgat cagcagcgag
      721 gatttctggc cggttttcct gctcattgcc gcctgcatct tcctgatcga tgtgttcatc
      781 cgccgcgtca ccgtccattt ctattggatc tggccggccc tggcattcgc ttataaccgc
      841 gttcgcggcc accagcagga agccgcgccg gacgagcggc tggcgcggct tcgcaaccgc
      901 aaggcggcga tttccagcca gatcgacgag cgccgggccg ctgcccgctt cgagccgcag
      961 ccggacggct tctccgagcc ccgggactac gagcaggtca tgcaggacgc ctccgccggc
     1021 ggcccgccgc ccgatcagcc ccggccgcaa gctcccaccc aagcttccgg cccgcagacc
     1081 gaacaggata catacaccga gcggctcttg gcggcgaaga agaaggcgaa gaaggaatag
     1141 ccgctttcca tgcgtattta cctcgacaat tgttgtctca atcggccgtt tgacgattgc
     1201 acacaggtta gaattgcact ggaggctgaa gcggtatctg caattctgag gctgtgtgaa
     1261 tcgggtgcct ggacgttgat cggttcagac gctctggatt tcgaggcggg acaagcgcct
     1321 ttgggaccgc ggaaagcatg gttagaggaa gtcttgctag gcgctggcga gttcgtcccg
     1381 atgagtgcgg acatcgtcgc gcgagcaacg gaactagaaa cacgagggct caaaaacatt
     1441 gatgcgattc atgtcgcttc ggcagaagca gtccaggcgg atatcttctg cacgtgcgat
     1501 gaccgcctac tgcggattgc caggaagcaa gccgatctac gagcccgagt cgcatcgcca
     1561 ctagaaatca ttcaggagct agagccgtga tccagcgaac tcaatcccta tctgaaatga
     1621 gtgagcgagc cgtccgcatg cttgtgcggg agctaggcgt tgtcgagacg cttcgttttt
     1681 tgaaccagtt ccgcgttgga aatggggatt acacgaagga tcgggacgcc ctttttgctc
     1741 agcaatcggt gcctgaactc gccgagcaaa tcaagcaatg gcgcgccgcg aacccaagac
     1801 cgacatcagg atgaccagcc cagcaatcct gtccgggatt cctctcatgc ccgtggatct
     1861 cgaaaccgtt gacttgttgg taacggccgg caagtacact gaggcagttc agctcttgag
     1921 gagtattgct gatgatcccg ccgcgccaag tcaagcgcga gtcgatgcct ttcagttcct
     1981 cggtaccatg acgaaggttg acccgagcgc aatgggtgag gactttgcac tgccgtattt
     2041 tcaacgtgcc ttacagttga aaccagatca cctttgggcg cttcagggta ttgtggcaac
     2101 gtttggggat cacgcaccgg accatcaaga taaggcagca cttctttttg cggccgaacg
     2161 gctgttggcc cgcgccaatg aattgccgca ggctgtggtg tcagaagtgc gtcaaagact
     2221 ggagagattt cacgctggac cgccaggtgc tgacgctgct ccgcattagc aagtgacata
     2281 cgaatcagtc acttttctgc tgcatcccga ttcgctatct tgctcgcact ctttccaatg
     2341 tgaccatcag aatagtcctt caaggagtct tcatgagtat cggcgagtcg atgcagcagc
     2401 aggcggagca gtttcgcaat cgctacagtt cggtgaagga gcaggtcggc cgcgtgatcg
     2461 tggggc
//
LOCUS       DASUGB010000103         4724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1679583, whole genome shotgun sequence.
ACCESSION   DASUGB010000103 DASUGB010000000
VERSION     DASUGB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679583"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..651
                     /locus_tag="VFV87_01940"
     CDS             145..651
                     /locus_tag="VFV87_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5102541.1"
                     /translation="METLLIIAGEVDQPRSLTFADLVAIPAEHQVADVSRLVAGRQGD
                     AVRLAGILALVGPKAAAKYLGLHSSTDDFHASIPLDAVAERALVIYRLNGQPLPVTRG
                     GPVRFFIPDFAACHTDEIDECANVKFVDRIELTATRGFDNRPHDGAEHEGLHAKAATS
                     GRGSSANS"
     gene            750..2102
                     /locus_tag="VFV87_01945"
     CDS             750..2102
                     /locus_tag="VFV87_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102542.1"
                     /translation="MGAAGAAAAGWLGCGGLNLDQPPEPALAPSDENPPAERAIDPRG
                     WPCLFGPELTSVSRETGLRIEWPAAGPPLLWQIPVGTGYSSPVLVDDDLIVLHRIENR
                     ELLSCFDAESGQPNWEHDWPTSFQCKYAYSSGPYGTPLIDGDVVFAAGAQGRLVAVSR
                     ADGALIWERDLQADRKLADGLFGFGPGFAIDAERFYLNAGGGEMNAGVIALDKRTGET
                     LWTATDHAMAYTTPRFMMVAGRRLLLVLTKVGLTCLDPATGDEHWTYEFHSKGVDTIN
                     AVAPAIQDNLVLLVAGPGPGSVCLRIRADLSYEEVWKDRRVLDSQFNTLVHEAGHIYG
                     FTARRQGGSIFKCLEFATGKLRWEYSSPLDRGQAIAADGRLILLGEHGLLTALALDPA
                     RLQVISTTAEPLLASPCYSSPALHRGLLYLRNESRLLCLRLRVTEAAFAPRRAPGIYR
                     "
     gene            complement(2138..3793)
                     /locus_tag="VFV87_01950"
     CDS             complement(2138..3793)
                     /locus_tag="VFV87_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5102543.1"
                     /translation="MSSRRGYFEALEPRAMLAGDAILHWNGVALDIVATDHTLPGLQE
                     HAGPTKTSRTLAIVHTAMFDAINSILGSYEPYLVKVIGTAGADIDAAVGQAAHDTLAA
                     LYQGQAAAIDEALVDWLDLIPDGPSENLGIALGKTVAQAILAARANDGSDLPTDYDYS
                     PGVIGRHQPDPVTIDVAGVVQSPLTPEWGAVKPFGVLHAASFLPPPPPSLTSAEYTAA
                     YNEVLQIGGDGIITPTIRTPEQTEIGTFWAYDGTEGLCAPPRLYNQIARVIAQQQGNT
                     EYQNARLFCLVNIAMADAGIVGWLCKYNDDFWRPVIGIREGDLDGNPNTAGVPNWTPL
                     GAPASNQTFPNDFTPPFPSYVSGHAVFGAALFQTLTRFYGRDNIAFSFTSDEFNGETT
                     NTNGVPRPEITRHFTSFSQASLENGVSRIYLGIHWRFDMTQGIAAGNKLADNIFNRLV
                     QPLQRPLAAGTAQNMLVTYAQTSASIASMNVGDGAQRQIVMLQSKTTPTQAIYYTANS
                     LTQFMQQTFRQPLPGEIVLIVNTTPLSGIAGLQSLLASFSLGN"
     gene            complement(3977..>4724)
                     /locus_tag="VFV87_01955"
     CDS             complement(3977..>4724)
                     /locus_tag="VFV87_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102544.1"
                     /translation="RNRWLPQEAQRKQLDALAALNKLHKDQRGHDPALDARIHSFELA
                     YRMQAEAAEAFDISREPQHVREAYGEGTQARQILIARRLIERGVRFVQVWHGQGQPWD
                     NHDDLKENHARLAGQCDRAIGALITDLKRLGLFEETLILWGGEFGRTPTVELPQAGAN
                     AGKINGRDHNHWGFTCWLAGGGVKGGQTYGGTDEFGFKAVENPVHVHDLHATILRLMG
                     FDHERLTYRYAGRDFRLTDVHGRVINELIA"
BASE COUNT          774 a         1503 c         1574 g          873 t
ORIGIN      
        1 cagatcgtgc tggaagtgaa ggcctggctg acggaacggg ccacgcagat tccggcctac
       61 gccgcctacc tgcagcggtg gggtgactgg accaatccct ggcagccggc tgccgaagcg
      121 gcggcggctg gctaaaatcc gggaatggaa actctgctta ttatcgcggg cgaagtcgat
      181 cagccgcggt cgctcacgtt tgccgatttg gtggccatcc cggcggagca tcaagtcgcg
      241 gacgtcagcc gtctcgttgc cggccgccag ggcgatgcgg tcaggctcgc cggcatcctc
      301 gccctcgtgg gcccgaaggc cgcggcgaag tatctcggct tacattcctc gaccgacgac
      361 ttccacgcca gcattccgct ggatgccgtg gccgagcggg cgctggtgat ctatcgcctg
      421 aacggccagc cgcttccggt caccaggggc ggaccggtgc ggttctttat ccccgacttt
      481 gccgcgtgcc atacggacga gatcgacgag tgcgccaacg tcaagttcgt cgatcggatc
      541 gagctgacgg cgaccagagg cttcgataac cgcccgcacg acggcgcgga gcacgaaggc
      601 ctgcacgcca aagccgcgac cagtggtcgc ggttcctcgg ccaactcgta aagccgcgcc
      661 ttcaccgtgt cttcacgcat tcttcgcgcg gcgtcgcaat aaaggctgcc gtgcgtgcaa
      721 tctccaggcg aaatctgctg gcgggctctg tgggggcagc aggtgccgcg gcggctggtt
      781 ggctcggctg cggcggcttg aatcttgacc agccgccgga accggccctc gcgccatcgg
      841 acgagaaccc gccggcggag cgcgccatcg atcctcgcgg ctggccgtgc ctgtttgggc
      901 ccgaactgac gagcgtgtcg cgcgaaacgg gcctccgcat cgagtggccc gctgccggcc
      961 cgccattgct ctggcagatt cccgtcggca ccggctacag ctcgccggtg ctggtcgatg
     1021 atgatctgat cgtgctccac cgcatcgaaa accgcgagct gctgtcatgc ttcgacgccg
     1081 aatccggcca gccgaattgg gaacacgact ggcccacatc ttttcagtgc aaatatgcct
     1141 acagcagcgg cccgtacggc acgccgctca tcgatggcga cgtcgtcttc gccgccggcg
     1201 cacaagggcg actcgttgcc gtgtcccgcg ccgatggcgc gctgatctgg gagcgcgatc
     1261 tgcaggcaga tcggaagctc gccgacggcc tcttcggatt tggaccggga ttcgcgatcg
     1321 acgcggagcg gttctacctc aacgccggag gcggcgaaat gaatgccggc gtgatcgccc
     1381 tcgacaagcg caccggcgaa acgctctgga ccgccaccga ccacgcgatg gcctacacca
     1441 cgccgcgctt tatgatggtc gcgggccgcc ggttactact tgtgctcacc aaggtcgggc
     1501 tgacgtgcct cgatcccgcc acgggcgatg agcactggac ctacgagttt cactccaaag
     1561 gcgtcgatac gatcaatgcg gtcgcgcccg ccattcagga caacctcgtc ctgctcgtcg
     1621 ccggtcccgg tcccggctct gtctgcctgc ggatcagggc cgatctgtcg tacgaagaag
     1681 tctggaaaga ccgccgagtg ctcgacagcc agttcaacac gctcgtccac gaggctggcc
     1741 acatctatgg cttcacggcg cgccgtcagg gcggctcgat tttcaagtgc ctggagttcg
     1801 cgaccggcaa gctccgctgg gagtattcct cgccgctcga ccgcggccag gccatcgccg
     1861 ccgacggccg gctgatcctc cttggcgaac acggcctctt gacggcgcta gccctcgacc
     1921 ccgcgcgact ccaagtcatc tccaccacag ccgagccgtt gctcgcatca ccctgctact
     1981 cctcccccgc tctccaccgc ggcctgctct atttgcggaa cgaatcgcgt ctgctgtgcc
     2041 tccgtttgcg ggtgacggag gctgcatttg ctccgcgtcg agcccctgga atttaccggt
     2101 agtgggatcg cttgacgggc cggcgctcac aaacctttca gtttcccagc gagaaactgg
     2161 cgagcaacga ttgcaggcct gcaatgcccg acagcggcgt cgtattgacg atcagcacga
     2221 tttcgcccgg tagcggctga cggaaggtct gttgcatgaa ctgggtgagc gaattggccg
     2281 tgtagtagat cgcctgtgtc ggcgtcgttt tggattggag catgacgatc tgccgctggg
     2341 cgccatcgcc cacgttcatc gaggcgatcg aggccgacgt ctgcgcgtag gttacgagca
     2401 tgttctgcgc cgtgccagcc gcgagcggcc gctgcaacgg ttgcaccagt cggttgaaga
     2461 tgttatcggc cagcttgttc cccgcggcga tgccctgcgt catgtcgaac cgccagtgga
     2521 tgccaaggta gatgcggctt acgccgtttt cgagcgaagc ctggctgaaa ctggtgaagt
     2581 ggcgggtgat ttcgggccgc ggcacgccgt tggtattggt cgtttcgccg ttgaactcgt
     2641 cagaggtgaa ggagaaggcg atgttgtcgc gtccgtaaaa ccgcgtcagc gtctggaaca
     2701 gcgcagcgcc gaaaacggcg tggccggaca catacgacgg gaacggtggc gtaaagtcgt
     2761 tcgggaaggt ctggttgctg gccggcgcgc cgagcggcgt ccagttcggc acgcccgccg
     2821 tgttgggatt gccgtcgagg tcgccttccc gaatgccgat gaccggccgc cagaaatcgt
     2881 cgttgtactt gcagagccag cccacgatgc cggcatcggc catggcgata ttgaccaggc
     2941 agaacaggcg ggcgttctga tactcggtgt tcccttgctg ctgggcgatg acccgggcaa
     3001 tctggttgta cagccgcggc ggagcgcaca gcccttctgt accgtcgtac gcccagaaag
     3061 tgccgatttc ggtctgctcc ggagtgcgga tggtgggcgt gataattccg tcgccgccga
     3121 tctgcagcac ttcgttatag gcagccgtgt attcggcgct agtgagtgac ggcggcggcg
     3181 gcggcaggaa gctcgcggca tggaggacgc cgaagggctt caccgcgccc cactcgggcg
     3241 tcagcggact ctggacgacg ccggccacgt cgatcgtgac cggatcgggt tgatggcggc
     3301 cgatgacacc gggggaatag tcataatcgg tgggcaagtc ggaaccgtcg ttggcgcggg
     3361 cggcgaggat cgcctgggcc accgtcttac ccagggcgat acccaggttc tcggatgggc
     3421 cgtcggggat cagatccagc cagtcgacga gcgcctcgtc gatcgccgcg gcctggccct
     3481 gatacaaagc ggccagcgta tcatgagccg cctggcccac ggccgcgtcg atatccgcgc
     3541 ccgccgtgcc gatcaccttc acgaggtacg gctcgtagct gccgagaatg gagttgatgg
     3601 catcgaacat cgccgtgtgc acaatggcca gcgtgcgcga agtcttcgtc ggcccagcgt
     3661 gctcttgcaa cccgggcagc gtgtggtcgg tcgcgacgat gtccagcgcc acgccgttcc
     3721 aatgcaaaat ggcatcgccg gccagcatcg ctcgcggctc cagcgcctca aagtagccgc
     3781 ggcgtgacga cacgcgccgt gcggaagatg aggacgtacg ccgagcgaga gactggaaca
     3841 tggcccacct gaacgaggaa gaaagaccgc agagacggcc tttgccggag caattcctac
     3901 ccgcggcatg gtaattggtg ccgaggccgg agtaaatagt ccggtcacgt gcactacgtt
     3961 ctgggcccgg aggcgtttaa gcgatcaact cgttgatcac gcggccatgc acgtctgtga
     4021 gccgaaaatc ccggccggcg tagcggtagg tgaggcgttc gtgatcgaag cccatgaggc
     4081 ggaggatggt ggcgtgcagg tcgtggacgt ggacggggtt ttcgaccgct ttgaagccga
     4141 attcgtcggt gccgccgtag gtctggccgc ccttcacgcc gccgccggcc agccagcacg
     4201 tgaagcccca gtggttgtgg tcgcggccgt tgatcttgcc ggcgttggcg cccgcttgcg
     4261 gcaattcgac ggtgggcgtg cggccgaatt cgccgcccca gaggatcagc gtctcttcaa
     4321 atagccccag ccgtttgagg tcggtgatga gcgccccgat cgcccggtcg cattggccgg
     4381 ccaggcgggc gtggttttct ttcagatcgt cgtggttgtc ccagggttgg ccctggccgt
     4441 gccagacctg cacgaaccgc acgccccgct cgatcagccg gcgggcgatg agtatctgcc
     4501 gggcctgagt cccttcgccg taggcttcgc ggacgtgctg gggctcgcgg ctgatgtcga
     4561 atgcttcggc ggcttcggcc tgcatgcggt aggccagctc gaagctgtgg atgcgggcgt
     4621 cgagcgctgg gtcatggccg cgctggtcct tgtgcagctt gttgagggcc gcgagtgcat
     4681 cgagctgctt tcgctgagct tcctgcggca gccagcggtt gcgg
//
LOCUS       DASUGB010000104         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_429193, whole genome shotgun sequence.
ACCESSION   DASUGB010000104 DASUGB010000000
VERSION     DASUGB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429193"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VFV87_01960"
     CDS             complement(<1..157)
                     /locus_tag="VFV87_01960"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5102545.1"
                     /translation="MTDSRDPRSNRSVAKTGVEGFDDVLGGGFTPDRLYLIEGVPGSG
                     KTTLAVQF"
     gene            complement(424..1251)
                     /locus_tag="VFV87_01965"
     CDS             complement(424..1251)
                     /locus_tag="VFV87_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102546.1"
                     /translation="MNASLWAMMLLLGQQPATSTPEPDNKVEAEQARAVAKLLAHEYE
                     IQLDQASDEKLKLQPEPVFRWLLQLDRRFYSDVYLWTHEGRPEVVAAITNVYGQRRAM
                     ETEIHSLSAHQPIMSHQGQIVWQPDRPGLELEPLSGAAPPQASAAARLRQMRALAAEH
                     AMTAVYDGTKKEDLRLLSTPVYRYASEKLGITDGALFAFVRGTDPEAFLMLEARQGEG
                     GVEWHSAFARFCGHCSLAAKRDGREVWNADVLPAKVNTDPKQPYFGLRRYSDFPVVK"
     gene            complement(1388..1741)
                     /locus_tag="VFV87_01970"
     CDS             complement(1388..1741)
                     /locus_tag="VFV87_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102547.1"
                     /translation="MLRFTLRELVLLVALAAMGLGWWVHANRQQREIRALEDDLLMQA
                     DFVDQFVSVLKHIGCEIPPDSDPRCATGGPYTRVFYPGYVTQQTGMLSSTCSGPRDQL
                     QRTLDGEFGVRFRNR"
     gene            complement(2075..2509)
                     /locus_tag="VFV87_01975"
     CDS             complement(2075..2509)
                     /locus_tag="VFV87_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102548.1"
                     /translation="MLFSLQPTRPSVHLLAAVVFLAGCGGERLDLVPVSGQITFDGKP
                     LKTGAVSFHPVETTGHVPTGIINSDGRYTLSTSYQPGAPPGRYKVVVHATEPVEVNPG
                     KASPGLPKSIIPTRYNDASTTPLDREVAAGNATDVYDLRLEK"
     gene            2711..3991
                     /locus_tag="VFV87_01980"
     CDS             2711..3991
                     /locus_tag="VFV87_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102549.1"
                     /translation="MFNIRLGSTKSYCDGVSRRSFLKVGAMTIGGLTLADLYRAEALA
                     GTGSSNKSIINVHLSGGPSHQDMWDLKPNAPAEFRGEFNPIATNVPGLEICEHFPMLA
                     TMGEKFAVIRSIVGSAGEHSNHQTHTAYHTRDLRNAGGRPALGSVVSRMFGEGAGGAP
                     AFISYNGGEPGYLGPVYKPYEPRGGSLRLVGGLTEERLDNRTNLLSSLDKIRRDADST
                     GQMEALDSFTRRAVDVVTSGKVADALDRSKFDAKDLERYGNGRRRDGVQFLTARRLVE
                     AGVRVVTFSWGGWDTHGGNFTTLKQQLPAMDQALAALLTDLSDRGLDKDVTVIVWGEF
                     GRTPRINSGAGRDHWPQVMAAFVAGGGMRLGQAIGSTTRDAGHAKDRPVDVQEIFATL
                     YRNLGIDVRSAQLFDTAGRPQYLVEKRDAISELV"
     gene            complement(4067..4933)
                     /locus_tag="VFV87_01985"
     CDS             complement(4067..4933)
                     /locus_tag="VFV87_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102550.1"
                     /translation="MPIAYSCPHCGKQYSVADQYAGQTGPCASCGKSISIPLVGAGAY
                     APRPAGGGGGGIAIAIAAIVVVLFLCAGGGVALILPAVQAAREAARRAQSSNNLKQIG
                     IAMHNYHDVYNTFPPAVVTGANGQPLYSGRVLLLPFMEQSNLFEAWAKDKAWDSPENM
                     ALSQTAIKVFQDPSNANTGNAAQTDYVFVTGTGTIFDPALGRGTRLQDITDGTSNTIM
                     VIEMRGSGINWAEPRDIDMSAAIPLPPSSHAGGNVVLFADGSVRNISTNVPPTTVHAL
                     TTRSGGEPATLP"
     gene            complement(4985..>5461)
                     /locus_tag="VFV87_01990"
     CDS             complement(4985..>5461)
                     /locus_tag="VFV87_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102551.1"
                     /translation="PLYSGRVLLLPFIEQNALFEQWDPTQAWDSPRNMPISQTALSIF
                     CDPSGDAPSPRTDYLFVVGTGTAFEESMKQNIASLKDGTANTLFMIEAKSTGINWAEP
                     RDFDASQPVPLPPGNHPRVNLGAFFDGSVKAISSDMPPEDVRALATRDGGETVFPN"
BASE COUNT          995 a         1608 c         1870 g          988 t
ORIGIN      
        1 ggaactggac cgccagggtg gtcttgcccg atccaggcac gccttcgatc aggtacaggc
       61 gatccggcgt gaagccgccg ccgagcacgt cgtcgaagcc ctcgacgccg gtcttggcaa
      121 ccgatcgatt ggagcgggga tctcgagagt cggtcatcgt tgttggtcgt tgttgccggg
      181 cgtggacgat ggccgcgtga ttacggacgc acgaccccgg cggaattatt ctatcggacc
      241 ggccggattc aattgggcaa cggctgggaa cgtaaatgag ggactgcgac aagttgtcgc
      301 ctttggcgcg aggccagcgc gaggttcggc cgtgggcgcc acttccgatc gcgcgttccc
      361 acgtccagtt cgtcgcacca ggagcgccga atcgagggcc tacgggcgga gcccgcgcgg
      421 agattatttc acgaccggga agtcggaata cctgcggagg ccgaagtagg gctgtttggg
      481 gtcggtgttg accttggcgg ggagcacgtc ggcgttccag acctcgcggc cgtcgcgctt
      541 cgccgcaagc gagcagtggc cacagaagcg tgcgaaggcg ctgtgccatt cgacgccgcc
      601 ctcgccctgg cgcgcttcca gcatcaggaa cgcttcagga tcggtgccgc ggacaaaggc
      661 aaacagcgcg ccgtcggtga tccccagctt ctcgctggca tagcggtaca ccggcgtgga
      721 gaggagccgc agatcctcct ttttcgtgcc gtcatagacc gcggtcatgg catgctcggc
      781 cgccagagcg cgcatctgtc gcaagcgagc ggcggccgag gcttgcggtg gcgcggcgcc
      841 tgaaagcggt tccaattcga ggcccggccg gtcgggctgc cagacaatct ggccctggtg
      901 cgacatgatc ggctgatggg ccgagagcga gtggatctcc gtttccatcg cccgccgctg
      961 cccatagaca ttggtaatgg ctgccacgac ctcgggccgc ccttcgtgcg tccagagata
     1021 cacgtcgctg tagaagcgcc ggtccagctg caggagccag cgaaagacgg gctcgggctg
     1081 gagcttcagc ttctcgtccg acgcctggtc gagctggatt tcatactcgt gggccagcag
     1141 cttcgcgacg gcccgggcct gctccgcttc gactttgttg tcgggttccg gcgtggaagt
     1201 cgccggttgc tggccgagga gcaacatcat ggcccagagc gatgcgttca ttgaaatgcc
     1261 tccgcgggaa tgaacgggca ccaccggccc ggcagccgaa gcaactgcat tgtcgcagac
     1321 cttgacgcga aataaaagac aacatgtctg tgccgagcgc gtgtgacggg cgggggtcgg
     1381 cgttcggcta ccgattgcgg aagcggacac cgaattctcc gtcgagggtg cgctgaagct
     1441 ggtcgcgagg gccggagcat gtggaggaga gcatgccggt ttgctgcgtc acgtagccag
     1501 gatagaagac gcgggtgtag gggccgccag tggcgcagcg cgggtcgcta tcgggtggaa
     1561 tctcgcagcc gatgtgcttg agcacgctga cgaattgatc gacgaagtcg gcctgcatga
     1621 ggaggtcgtc ctccaaggcg cggatctcac gctgttgtcg attggcatgg acccaccagc
     1681 ccaggcccat cgcggccaag gcgacgagga ggacgagctc gcggagggtg aagcggagca
     1741 tgggggtagg ggtttagagg tcaggggacg tgtcgaatct ttcgacacgc gggaataaat
     1801 gcagggccat gcgcatctat atatagtttg tacatgatca gttgtgatct tcagcgtgtc
     1861 ggccgggtgg tcgttgggaa tcgcccaaca ccgctgcaaa caagacgggt gtcggacaaa
     1921 aatggacgaa aattggttga caacgcgcaa cagcgcaaca tcctcgtacg gggggtgttg
     1981 cgcggtgcaa gtgcgttctg ataaacgagt tatggaaatg tcgtgcggtc gcggcggcgc
     2041 gaaaaaaagc ggaaaagttg cgcttgcgca gcgcttactt ctccagccgc aggtcgtaaa
     2101 cgtcggtggc gtttcccgcg gcgacctccc ggtcgagcgg ggtggtggaa gcgtcgttgt
     2161 aacgcgtcgg aatgatggat tttggcaggc ccgggctggc ttttcccgga ttgacctcga
     2221 cgggctcggt ggcgtgaacg acgaccttgt agcgcccggg cggagcgccg ggttggtagc
     2281 tggttgaaag ggtatagcgg ccgtcggaat tgatgatgcc ggtggggacg tggccggtcg
     2341 tttccacggg atggaacgaa acggccccgg ttttcagcgg cttgccgtca aaggtgatct
     2401 gcccgctgac ggggacgaga tccagacgct cgcccccaca gccggccaga aaaacaacgg
     2461 cagcgagaag gtgaacagac ggacgggtgg gctgcaaaga aaaaagcatc ggaaagtgtt
     2521 gatttgacct gccgggagtg atataagcgc ggttgtacgt caagatagac cgatccagcc
     2581 ccgtcagata gcgatctcgc tgggcgagag ctggccgcgt aaccagccta ccggtccatg
     2641 tcataacgta ttagcccgcc tgccgtcttg ccgaacggaa cccacccacc ccaacctcag
     2701 gaatctggcc atgttcaaca ttcgccttgg ctcgacaaaa tcgtattgcg acggagtttc
     2761 gcgtcgttcg ttcctcaagg tcggggcgat gacgatcggc ggcttgacgc tggccgatct
     2821 gtatcgagcg gaggcgctgg ccgggaccgg atcgtcgaac aagagcatca tcaacgtcca
     2881 tttgagcggc ggtccttcgc accaggacat gtgggacctc aagcccaacg ccccggcgga
     2941 atttcgtggc gagttcaacc cgatcgcgac caatgtgccg ggcttggaaa tctgcgagca
     3001 cttcccgatg ctcgccacga tgggcgagaa gttcgcggtg atccgctcga tcgtcggttc
     3061 ggcaggcgaa cattcgaacc accagacgca cacggcctat cacacccgcg acttgcgcaa
     3121 cgccggtggc cggccggcgc tggggagcgt cgtctccaga atgtttggcg aaggcgccgg
     3181 cggcgcgccg gcgttcattt cgtacaacgg cggcgagccc ggctatctcg gcccggtgta
     3241 taagccctat gagccgcgcg gcggcagcct gcggctggta ggcggcctga ccgaagagcg
     3301 gctggacaat cgcacgaacc tgctcagctc gctcgacaag attcgccgcg acgcggattc
     3361 gaccgggcaa atggaagccc tcgattcgtt cacccggcga gccgtcgatg tcgttaccag
     3421 cggcaaggtg gccgatgcgc tggaccgcag caagttcgac gcgaaggacc tggagcgtta
     3481 cggcaacggc cgccggcgcg atggcgtgca gttcctcacc gctcgccggc tggttgaggc
     3541 gggcgtgcgg gtcgtcacgt ttagctgggg cggctgggat acgcacggcg gaaacttcac
     3601 gacgctcaag cagcaattgc cagccatgga ccaggccctg gcggccctgc tcaccgattt
     3661 gtcggatcgc ggcctggaca aggacgtgac ggtgattgtg tggggtgaat tcggccgcac
     3721 accgcggatt aacagcggcg ctggccgcga ccactggccg caggtgatgg ccgccttcgt
     3781 cgccggcggc ggcatgcggc tgggccaggc gattggcagc acaacgcgcg acgccggtca
     3841 cgcgaaggat cgtccggtcg atgtgcagga gatcttcgcc acgctgtatc gcaacctggg
     3901 aatcgacgtc cgctcggcgc agctcttcga tacggccggc cgccctcaat acctggtgga
     3961 gaagcgcgac gcaatcagcg agctggtgta agcggggcag gagcaacagc ctggctgggg
     4021 caacagcctg cctggggcgc ctggggcgcc tggggcgcag gggcgcctag ggcaatgtgg
     4081 ccggctcgcc gccgctgcgg gttgtcagtg cgtgaacggt cgttggcggc acgttcgtcg
     4141 agatattccg taccgaaccg tcggcaaaca gtacgacgtt tccgccggcg tggctgctcg
     4201 gcggcagcgg gatggcggcg ctcatgtcga tatcgcgcgg ctcggcccag ttgatgccgc
     4261 tgccgcgcat ttcgatgacc atgattgtgt tcgacgtgcc atcggtgatg tcttgcagcc
     4321 gcgtcccgcg ccccagggcg ggatcgaaga tcgtgcccgt tccagtgacg aacacgtaat
     4381 cggtctgggc agcgttgccg gtgttggcgt tggacggatc ctgaaagacc ttgatggccg
     4441 tctggctgag ggccatgttc tccgggctgt cccaggcttt gtccttggcc caggcttcaa
     4501 acagattgct ctgctccatg aagggcaaca gcagcacgcg tccgctatag agcggctggc
     4561 cattggcgcc tgtcacgacg gcgggcggga acgtgttgta aacgtcgtga tagttgtgca
     4621 tcgcaatgcc gatctgcttg agattgttcg acgattgcgc ccgccgcgcg gcttcgcgcg
     4681 ccgcttgaac cgccggcaga atcagcgcta caccgccgcc ggcacagaga aagagcacaa
     4741 cgacaatggc agcgatcgcg atggcgattc caccgccccc gccgccggct ggccgcggag
     4801 catacgcgcc ggctcccacc agtggaatcg aaatcgactt gccgcaactg gcgcacgggc
     4861 cggtttggcc ggcatattga tccgccacgg aatactgttt gccgcagtgc gggcaactat
     4921 aagcaatcgg catcgaacgt ctcctgtggt gcggcgctca ctattagttc caaatcaacg
     4981 gaggttagtt cgggaatacg gtttcaccgc cgtcgcgcgt ggccaaggcc cgcacgtctt
     5041 ccggcggcat atcgctggag atcgccttga cgctgccgtc gaagaaggcg cccaggttca
     5101 cgcgcggatg attgccgggc ggcaaaggca cgggctggct ggcgtcgaaa tcgcgtggct
     5161 cggcccagtt gatgcccgtg ctcttggctt cgatcatgaa cagcgtgttc gcggtcccgt
     5221 ccttgagcga cgcgatgttc tgcttcatgg actcctcaaa cgccgtgccc gttccgacga
     5281 cgaacagata gtcggtgcgg ggcgacggcg catcgccgga agggtcgcag aagatactga
     5341 gagccgtctg gctgatgggc atgtttcgcg ggctgtccca ggcttgcgtc ggatcccact
     5401 gttcaaagag cgcattctgt tcgatgaacg gcagcagcag gacgcgcccg ctatacagcg
     5461 g
//
LOCUS       DASUGB010000105         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1262958, whole genome shotgun sequence.
ACCESSION   DASUGB010000105 DASUGB010000000
VERSION     DASUGB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262958"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VFV87_01995"
     CDS             <1..1978
                     /locus_tag="VFV87_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5102552.1"
                     /translation="IYGWRGGVAAIFGALDGELPGLSQSGLDISYRSSQPIIDAVNEV
                     FQNLTKHPNLDRYAEAVAEWQAQYPSHQTAKTDLPGHVTLTTAPPAGEDEAPDDVLFE
                     HAADRIAAAMREAPTASIGVLVRTNKAVAHLIYLLRQRDILASEEGGNPLSDSPAVEL
                     ILSLLKLADHPGDSVARFHLATSPLAAPLELADYRDDRSAARTSQDLRQQLLEDGYGE
                     TIHHWARKLAPSCDRRDLSRLQQLVELAYDYQPRSSLRADQFIALVQSKRIADPAASH
                     IRVMTIHQAKGLEFDIVFLPELGARIAGQRDQYVAGRPSPTEPFDLVCRMANENVRQF
                     FPPRLAKLFEEDAKDEVTESLCVLYVAMTRAIHALHMILPPAAANERSLPKTYAGLLR
                     AALAAGTPATGTKMLYEHGNPQWFATLPAPLVGEQPGARGSSAAESPSPKIQFAPPLK
                     SRDRNLERTSPSALEGGSKFRAAHLLAQRPTDAFALGTLIHAWLAEIEWLDDGLPPED
                     RLREIAARLRPDIGTLADHLDAPLANLRRWLTAPAIVQALSRKNYDKNAQLQVLREHP
                     FAIRDGDTLLSGSIDRLVLVRNGGHIVAADILDFKTDELPLGDAAAIAARTQFYRPQI
                     EAYASAVANIFRCARPAISMRLLFLNAGVGQYLA"
     gene            2047..>3185
                     /locus_tag="VFV87_02000"
     CDS             2047..>3185
                     /locus_tag="VFV87_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102553.1"
                     /translation="MRLSWEGAGAESARLYPRPKLCVDMKIQELLILLPCHSLEDFPT
                     YHEGDDAHSLLASWSALWHPELLAAAGQALNWRRIEDPPSDVAGRVIVVPAVCVQRLP
                     TGFAQRVKDEGGLLIRKTSSRDEIVAAALAALREEAKHQDAEEPSASAPESRTPNPEP
                     RSPVPDDLAADFLALGYCYLQIELLTRQMRYSSNLDETYFKSTAVAAAQAAVAGNSEL
                     AREKLSACFSVLAEERDHYYPVDAFILDLNLTAATTLGEALRAELARPSPTNVLISAD
                     LLAEMAEKHPDSLAALREGIEAQRIGLIGGEATETRLPLLSHETILAELTRGIAKYEQ
                     LLGARPQVFGRWRYGLTPHLPGILHKLGYRGALHAGFEEGKTPDGV"
BASE COUNT          576 a         1164 c          953 g          492 t
ORIGIN      
        1 gatctacggc tggcggggcg gagtcgccgc gatcttcggc gcgctcgatg gcgagttgcc
       61 cggactgtcg cagagcggcc tcgatatcag ttaccgctcc agtcagccga taatcgacgc
      121 cgtcaacgag gtcttccaga acctgacgaa gcacccgaac ctcgaccgct atgccgaggc
      181 ggttgccgag tggcaagcac agtatccgtc gcaccagacc gccaagaccg acctgcccgg
      241 ccacgtcacg ctgaccaccg cgcccccagc gggcgaagac gaagctccgg acgacgtgct
      301 gtttgaacac gccgccgatc gaattgccgc tgcgatgcgc gaagcaccga ccgcctcaat
      361 cggcgtcctt gtacggacca acaaagccgt cgcccacctc atttatctcc tgcggcagcg
      421 cgacatcctc gccagcgaag aggggggcaa cccgctcagc gattcgccgg cggtcgaact
      481 gatcctgtcg ctcctgaagc tggccgatca tccgggcgac agcgtggccc ggtttcatct
      541 ggcgacttcg ccgctggctg caccactgga gctggccgat tatcgcgacg atcggtccgc
      601 cgcgcgaacc agccaagact tgcggcagca gctcttggag gacggctacg gcgaaacgat
      661 tcaccactgg gcgaggaagc tggccccatc ctgcgatcgc cgcgacctca gccgcctgca
      721 gcagctcgtc gagctggcct acgactatca gccgcgaagc tcactccgcg ccgatcaatt
      781 catagccctc gtgcagagca agcggatcgc tgaccccgcc gccagccata tacgggtgat
      841 gacgattcac caggccaagg ggctggaatt cgacatcgtc ttcctgccgg agctgggcgc
      901 aaggattgcc ggtcagcgcg accagtacgt cgccggccgc cccagcccca ccgagccgtt
      961 cgacctcgtc tgccgcatgg cgaacgaaaa cgtgcgccag ttcttcccgc cgcggttggc
     1021 gaagctgttc gaggaagatg cgaaggacga ggtgaccgaa tcgctgtgcg tgctgtatgt
     1081 ggcgatgacc cgcgcgatcc acgcgctgca catgatcctg ccgccggccg cggcgaacga
     1141 gcgttccctt cccaaaacgt acgctggcct cctccgcgcc gccctggccg cgggcacacc
     1201 cgcgactggc acgaaaatgc tttacgagca cggcaatccg cagtggttcg ccactctgcc
     1261 tgctcccctc gtgggagagc agccgggggc gagggggagc agcgccgccg agtccccatc
     1321 gccaaaaatc caattcgccc caccgctcaa gtcccgcgac cgcaacctgg agcgcaccag
     1381 tccctcggcc ctcgaagggg gctcgaaatt ccgcgccgcg cacttgcttg cgcagcgacc
     1441 gaccgacgca ttcgccctcg gcacgctcat ccacgcctgg ctcgccgaaa tcgagtggct
     1501 cgacgacggg ctaccgcccg aggaccgcct ccgcgaaatt gccgcccgac tccgccccga
     1561 catcggcacg cttgccgacc acctcgatgc gccgctggcc aatctccgcc gctggctcac
     1621 cgcgccggcc atcgtccagg ccctgtcccg gaaaaactac gacaagaacg cccagctcca
     1681 agtcctccgc gagcacccct tcgccatccg cgacggcgac acgctcctct ccggcagcat
     1741 cgaccgcctc gtcctcgtgc gcaacggtgg ccatatcgtc gccgcagaca ttctcgactt
     1801 caaaaccgac gagctgccct taggcgacgc tgccgcaatc gccgcacgta cgcagttcta
     1861 tcgtccgcag atcgaagcct acgcgagcgc agtggccaac atatttcgct gtgctcgccc
     1921 agccatttca atgcggttgc tcttcttgaa tgcgggggtc gggcagtatc tcgcctagga
     1981 gtggatcaag ttgccgtgga ccggccccgc cccgcgatgc ggtgcgctta tcgacgaccg
     2041 ccaaggttgc ggctatcgtg ggagggtgct ggggcggagt cggcccggct ctatccgcgg
     2101 ccgaaattgt gcgtcgatat gaagattcaa gagctgctga tcctcctgcc gtgccacagc
     2161 ctggaggact ttccgaccta ccacgagggg gacgacgccc acagcctgct cgcaagctgg
     2221 tcggctctct ggcatccgga attgctggcg gcggccgggc aggccctcaa ctggcggcgg
     2281 atcgaggacc cgcccagcga cgtggccggc cgcgtgatcg tcgtcccggc ggtctgcgtc
     2341 cagcggctgc cgaccggctt cgcccagcga gtcaaggacg aaggcggcct gttgatccgc
     2401 aaaaccagca gccgcgacga aattgtcgcc gcggcgctcg ctgcgctgcg cgaagaagcc
     2461 aagcaccaag atgccgaaga accaagcgcc tcggctcccg aatcccgaac cccgaacccc
     2521 gaaccccgct cccccgtccc cgacgacctc gccgccgact tcctcgccct cggctactgc
     2581 tacctgcaga tcgagctgct gacgcggcag atgcggtact cgagcaacct cgacgagacc
     2641 tacttcaaga gcacggcggt cgccgccgca caggctgccg tggcgggcaa ctcggaactc
     2701 gcccgcgaaa agctctcagc ctgcttcagc gtgctggccg aagagcgcga ccattactac
     2761 ccggtcgatg ccttcattct cgatctgaac ctgaccgccg cgacgacgct gggcgaagcg
     2821 ctgcgggccg aactggcccg gccctcgcca accaatgtgc tcatctccgc tgacttgctg
     2881 gccgagatgg cagaaaagca ccccgattcg ctcgccgcgc ttcgcgaggg aatcgaggcg
     2941 caacgcatcg gcctgatcgg cggcgaagcg accgaaacgc ggctgccgct cctttcgcac
     3001 gaaacgatcc tcgccgagct gacgcgcggg atcgccaaat acgagcagct tctcggcgcc
     3061 cgcccgcaag tcttcggccg ctggcgctac ggcctcacac cgcacctgcc gggcattctg
     3121 cacaagctcg ggtatcgcgg ggcgctgcac gccggctttg aggaaggcaa aacgcccgac
     3181 ggcgt
//
LOCUS       DASUGB010000106         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_152050, whole genome shotgun sequence.
ACCESSION   DASUGB010000106 DASUGB010000000
VERSION     DASUGB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152050"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..970)
                     /gene="msrB"
                     /locus_tag="VFV87_02005"
     CDS             complement(470..970)
                     /gene="msrB"
                     /locus_tag="VFV87_02005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5102554.1"
                     /translation="MVQVHVFNKSGELVGPVESPKLELSEAEWRTRLSPEQFRILRTS
                     GTERPFCGTLLDNKQHGVYACAGCGLPLFSSEHKFHSGTGWPSFFHPIAPGNVAEKPD
                     DSYGMTRVEINCARCDGHLGHVFPDGPPPPRLRFCLNSESLQFTPKEELHSLADSACD
                     GTDFRV"
     gene            1127..1597
                     /locus_tag="VFV87_02010"
     CDS             1127..1597
                     /locus_tag="VFV87_02010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5102555.1"
                     /translation="MFLAFRLATPADQPDIERLVLAAFEPITWQKKLDARFGPLNGHD
                     WQARWRQRLEKIFATQIVLVGETASGELAAVATATIDREAKLGFIDVLAVSRDQQGQG
                     LGRAMLRAAMEHLRSLGCQYVNLDCLTDNESGNALYASEGFAEVARHVRWFRKI"
     gene            complement(1643..1870)
                     /locus_tag="VFV87_02015"
     CDS             complement(1643..1870)
                     /locus_tag="VFV87_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102556.1"
                     /translation="MKPLYETVNQHMRRVASQSAPFRPELRRSLWPLVVAFVFACGLI
                     VVTGILFGEAAAEIARTYADWIQHLASPLVR"
     gene            2017..>2295
                     /locus_tag="VFV87_02020"
     CDS             2017..>2295
                     /locus_tag="VFV87_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102557.1"
                     /translation="MCPQIRCLHAMVFVAAFAAGIARGADDPHSEGAPRVTDVPVELR
                     AKHELDPFYVKHADYQGYPILSSAKVSDAALLEARHLIGQMLAGRDDIV"
BASE COUNT          441 a          693 c          735 g          426 t
ORIGIN      
        1 cttgcaacaa caccactaca ggcaagcgag gcctttcgtg tccagtgcag ttactccctg
       61 tgcaccaagg agttagaaca gttcaagtgc cattcgaaag aggcaccatt acggttgtca
      121 gtcttgacga gccaattgat ccggacgttt tcacgctaaa aggaatggac attccgaacg
      181 gccagccagt tgcagtgttg cctcgtggtc cgggaaggta cgtgtggaac ggagatgcga
      241 ttgttcgcga caccgaagcg aaacggcctg aagcaaatca gggggcgtcg acgtttccca
      301 acgggtatga cgtgggagcg gctttcttcg ccgtcctcgc ggttgccgcg acgcatcttg
      361 tggttcgacg acttcctcgt tggtccgtcg aagcgccgcg agcacatccg caaacgggct
      421 aacgaacttg tcgcgacgcg gaagaaattg cctcgaatcc ggggaccgat taaacgcgga
      481 agtcggtccc atcgcacgcc gaatctgcaa ggctgtgcag ttcctctttc ggtgtgaact
      541 gcagcgactc ggaattcagg cagaaccgca gccgcggggg aggcgggccg tcgggaaaga
      601 cgtgccccag gtgcccgtcg cagcgggcgc agttgatttc gacccgcgtc atgccgtagc
      661 tatcgtccgg cttctcggcg acgttgcccg gcgcgatcgg gtgaaagaag ctcggccagc
      721 cggtgccgga atggaacttg tgctcggacg aaaagagggg cagcccgcag ccggcacagg
      781 catagacccc gtgctgcttg ttatcgagca gcgtgccgca gaacggccgc tccgtgccgc
      841 tggtgcgaag aatgcggaac tgctcgggcg agagccgcgt tcgccactcg gcttcgctca
      901 actccagctt aggcgactcg accggcccca ccagctctcc ggacttgttg aaaacgtgga
      961 cctggaccat tgcagtttgc tcctgggctc ttcattctat gcgatgccgg ctgggcaggg
     1021 atgttacaag ccaagccgta aggaaaagga aaccgcggat gtcgcggaat gcgcggatgt
     1081 ggagatccgc gtttatccgg gaaatccgcg gcgataaatc tccgccatgt tcctcgcgtt
     1141 tcgcctggcg acgcccgccg atcagccgga tatcgaacgg ctcgtgctgg cggcgttcga
     1201 gccgatcacc tggcagaaaa agctcgacgc ccgcttcgga ccgctcaacg gccacgattg
     1261 gcaagcccgc tggcggcagc ggctggagaa gatctttgcc acgcagatcg tcctggttgg
     1321 cgaaacggca tcgggcgagc tagccgcggt cgccacggcc acgatcgatc gcgaggcgaa
     1381 gctcggtttt atcgacgtcc tggccgtcag ccgcgatcaa cagggacagg gattaggtcg
     1441 tgcgatgctg cgcgcggcaa tggagcattt gaggagcctc ggctgccagt atgtaaatct
     1501 cgactgcctc accgacaatg agtcgggcaa cgccctctat gcgagcgagg gcttcgccga
     1561 agtcgcccgc cacgtccgct ggtttcggaa gatctgatgt aagtcgcgat gcggcttgac
     1621 cgccggttat gtaaccagcc ggctacctaa ccagcggcga tgccagatgc tgaatccagt
     1681 cggcgtacgt ccgagcgatc tccgcggccg cttcgccgaa cagaatccca gtcaccacga
     1741 tcaggccgca ggcgaagaca aacgcgacta ccagcggcca tagcgaccgg cgaagctcgg
     1801 gccgaaacgg cgcgctttgg ctggcgacac gccgcatgtg ctggttaacg gtttcataaa
     1861 gaggtttcat ggcctggcct ccctttgttt ctacgcacgt cacctgccgg gagtttgggc
     1921 cgccgcggag ccgagcgtac cgctcgcctt gctccgcgcc gcctgttagg gtgatcccat
     1981 gcgacattgc tgccccagcg cagggagaat gactcaatgt gtccgcaaat tcggtgcctt
     2041 cacgcgatgg tctttgtcgc ggcgtttgct gcgggaatcg cccgcggtgc cgatgacccg
     2101 cactcggagg gggcgccgcg cgtgacggac gtgccggtag agctgcgggc caagcacgag
     2161 ctggacccgt tttacgtaaa gcacgccgac taccagggct atccgatcct gagctcggcg
     2221 aaggtctcgg atgcggctct gttggaggcg cggcatctca tcggccagat gctggcgggc
     2281 cgcgacgaca tcgtg
//
LOCUS       DASUGB010000107         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1958714, whole genome shotgun sequence.
ACCESSION   DASUGB010000107 DASUGB010000000
VERSION     DASUGB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958714"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..2036)
                     /locus_tag="VFV87_02025"
     CDS             complement(219..2036)
                     /locus_tag="VFV87_02025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5102558.1"
                     /translation="MLSATILLAAVSPIFAQRDPIRLTHGPMLGKPTAHSVAVWGRTS
                     EAGQFVVHYGTDALRLDQVSQPAITALDRDDTGVAQLRDLRPDTRYFYEIWVNGRPHG
                     LPGSFRTLPSATEVRNEKYNPQGLFNFRFEIGSCANQNPLSGLGPRLPTYENLNRDWA
                     DKVHFHIMNGDWLYEELRNYPVEAWRLEQGLDALPRKVEVMPTVVGVWENYKLYLSRG
                     VELAKWHRNVPSYFTFDDHELVNDIYASGEAGRRDRRTVFRDIGVAAWHDYLGWANPA
                     AFEHPVHFSRGRMQAGSDRLVDPATDFTKLPLKEMLNLHVHWGTPQAGVDDIRFDDDR
                     GHKNSAVYDVVAVLDPHTLRLHMPAKVTDEVTYSIGRRNYGKFRVSNCDFFLLDTRGD
                     RDMHDVQHRDKPGVSLIGKQQRQWLLDSMRASDADFFFVISTVPFMIPHTGSGGAELN
                     PENKEESWTGFFDERETLIAEWEKLGKKVFVMTGDLHNSFAIKVTDHIWEFCCGPHNS
                     VNHVPANDESGRPATGRWKFGPRDCDIRWSSYILPDLPRTERMYPHFCIVQVNNVFNM
                     PQKLGDTRWVAYPHPQVVFQYYDGRTGELAYAEAISVER"
     gene            complement(2195..3238)
                     /locus_tag="VFV87_02030"
     CDS             complement(2195..3238)
                     /locus_tag="VFV87_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102559.1"
                     /translation="MKRNWLPYVAIAAALVAGSICSTAFAGPPSFKFGGNSGGGNFRI
                     GGTNNSGGNNLSRNLGNVRIQQGTNNSLPKIGNFNGSSGGMQTQIKKLPGNLGNLNGN
                     LGQAIGNSNRVTTPKITINPGVVNQIKQGNLNGNGNGHIGQAIGNAVLNGNKNVKINP
                     GFNKGNLGQAIGNAIVGGGSQKSIQCGPVKVGCQPWWNDCHHFHHDHCYKPVYPVCSQ
                     TIIVQVPVEQPVAVAQPAMQVPVGATLTLQSLGLGDAAGQVMIQVDKIAIPAMVNEWT
                     NDAVTTTLPMLGLGGQVIADIVMVRADGTVANSIKVELINAQPQAQDAQVVPASASAP
                     AGAGDAAAALFAQ"
     gene            3631..>3965
                     /locus_tag="VFV87_02035"
     CDS             3631..>3965
                     /locus_tag="VFV87_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102560.1"
                     /translation="MRRLGVLAVIVSGLLAATPTSRADRLDEIIAAQNQAAGLKLAAT
                     PIVDDEVFLRRVFVDLIGRIPTTGEVNAFTAADPKDRREKLVEKLLADERFADRWTIF
                     YADMLRLRS"
BASE COUNT          737 a         1182 c         1233 g          813 t
ORIGIN      
        1 acggcctggc gaaggacgaa gcgaagcggc aggtcgacga attcgagaac gcgtgcggct
       61 gctgagaaac ggataagagc gctggccggc ggacagtcgc cgcgctcgta ccctcgaagc
      121 agctagcgcg tgggcacgct ccggcggata agtcttcggt cccgcagctc gattgagctg
      181 cctcgctggg cgaaggcgct aggagaattg ccacccccct atcgctcgac cgaaatggcc
      241 tcggcgtagg ccagttcgcc ggtgcggccg tcgtagtatt gaaagacgac ctgcggatgc
      301 ggataggcga cccagcgcgt atcgccgagc ttttgcggca tattaaagac gttattgacc
      361 tgcacaatgc agaagtgcgg gtacattcgc tcggttcgcg gcagatccgg caggatgtag
      421 ctgctccagc ggatatcaca gtcgcggggc ccgaatttcc acctgccggt ggctgggcgt
      481 ccgctttcgt cgttggccgg tacgtggttg acgctgttgt gcggcccgca acagaactcc
      541 cagatgtgat ccgtgacttt gatcgcaaag ctgttgtgca agtcgcccgt cattacgaac
      601 accttcttgc cgagcttctc ccactcggca atcagcgtct cgcgctcgtc gaaaaagcca
      661 gtccaagact cctccttgtt ctccgggttg agttccgccc caccggagcc cgtgtgcgga
      721 atcatgaatg gcaccgtcga aatcacgaag aagaaatcag cgtcgctggc acgcatcgaa
      781 tcgagcagcc actggcgctg ctgcttgccg atcaggctga cgccgggttt gtcgcgatgc
      841 tgcacgtcgt gcatgtcgcg gtcgccccgc gtatccagca ggaagaaatc gcagttggag
      901 acgcggaatt tgccgtaatt gcggcggccg atcgaatagg ttacttcgtc cgtcaccttc
      961 gccggcatgt gcaggcgcag cgtgtgcgga tcgaggactg cgacgacgtc gtacaccgcg
     1021 gaattcttgt ggccgcgatc gtcgtcgaaa cggatgtcgt cgacccccgc ctgcggcgtc
     1081 ccccagtgga cgtgcaggtt cagcatttcc ttcagcggca gcttcgtgaa atcagtcgcc
     1141 ggatcgacca gcctgtcgct gccggcttgc atccgaccgc ggctgaagtg gacgggatgc
     1201 tcgaatgccg ccggattcgc ccagcccaga taatcgtgcc aggccgcgac gccgatgtcg
     1261 cggaagaccg tgcggcggtc gcggcggccg gcttcgccgg aggcgtaaat gtcgttgact
     1321 aactcgtggt cgtcaaacgt gaagtagctc ggcacgttcc gatgccattt tgccagctcg
     1381 acgccgcgac tgagataaag cttgtaattc tcccacacgc cgacgacggt cggcatcact
     1441 tcgacttttc gcggcagtgc atccaagccc tgctccagcc gccaggcctc aaccgggtag
     1501 tttcgcagct cctcgtacag ccaatcgcca ttcatgatgt ggaagtggac tttgtcggcc
     1561 caatcgcggt ttagattttc ataagtcggc aagcgcgggc ccagaccgct cagcgggttc
     1621 tgattggcgc acgagccgat ctcgaaacga aagttgaaca gaccctgggg attgtatttc
     1681 tcgtttcgca cctccgtcgc gctcggcagc gtgcgaaagc tcccgggcaa gccgtgcgga
     1741 cggccattga cccagatctc atagaagtac cgcgtatccg gccgcagatc ccggagctga
     1801 gcaacgcccg tatcgtcgcg gtcgagcgcg gtgatcgccg gctggctgac ttgatccagc
     1861 cgcaaggcat ccgtcccgta atgcacaacg aactggcccg cctcgctggt ccgtccccac
     1921 accgcgaccg aatgggctgt cggcttgccg agcatcggtc cgtgcgtcag ccggatcgga
     1981 tcgcgctggg caaagatggg cgaaacggcc gccaacagaa tggtggctga cagcataaga
     2041 atggtgctcg tgcgcatgag cgccattgtc gaacgccgaa acaaaaaagc aaccgaatcg
     2101 cggtttctaa acgaaagtca agaaacgaga accgggagca cacgtgctcc cggttcgttg
     2161 attcaatctg ggtcgggcgt cgcccgatcg agtcttactg ggcgaacagg gccgcggccg
     2221 catcgcctgc accggcgggc gccgaagccg aggcaggcac cacctgagcg tcctgagctt
     2281 gcggctgggc gttgatcagc tcgaccttga tcgagttggc cacggtgccg tcggcccgga
     2341 ccatcacgat gtcggcgatc acctggccgc ccaggcccag catcggcagg gtcgtggtga
     2401 cggcgtcgtt ggtccactca ttgaccatcg cggggatcgc aatcttatcg acctggatca
     2461 tcacctgccc ggccgcatcg cccaggccca gcgactgcaa ggtcagcgtc gctccgaccg
     2521 gaacctgcat tgccggctgg gccacggcca ccggctgctc gacgggcacc tgcacgatga
     2581 tcgtctgcga gcaaaccggg taaaccggct tgtagcagtg atcgtggtga aagtgatggc
     2641 aatcgttcca ccagggctgg cagccgacct tgaccgggcc gcattgaata ctcttctggc
     2701 tgccgccacc gacaattgcg ttgccgatcg cctggccaag gttgcccttg ttgaatccgg
     2761 ggttgatctt cacgttcttg ttgccgttca ggacggcgtt gccgatggcc tgaccgatgt
     2821 ggccattgcc gttgccgttc aggttgccct gcttgatctg gttgaccacg ccggggttaa
     2881 tcgtgatttt cggggtggtc acgcgattgg aattcccgat cgcctgtccg agattgccgt
     2941 tcaggttgcc gaggttcccg ggcagcttct tgatttgcgt ttgcatgccg ccggagctgc
     3001 cgttgaaatt gccaatcttc ggcaggctat tgttcgttcc ctgctggatg cggacattgc
     3061 ccagattgcg gctgagattg tttccaccgc tattgttcgt cccgccgatg cggaaatttc
     3121 cgccgccgct gttgccgcca aacttgaagc tcggtgggcc ggcgaaggcc gtgctgcaaa
     3181 tgctgccggc gacgagggcg gcggcgattg cgacgtaagg aagccagttg cgtttcattg
     3241 taaagtcctt tgcgacttgg agttgtgtca attgctggcg ggcccgtaat tgccggcctc
     3301 taagcatccg ggcgcaaagc agggcggaat gttacgcctc ggactggctt tttccgctca
     3361 aagctgaatt gcccaggatt tgatccgcga acgccaaatt gtcgcgattc ttggcgaagg
     3421 ctgatgcgtc aaggagcctc gcaacccgtt gccgccgctg gagttgtgaa atggctgagt
     3481 acggcgaatc agattccccg ttaattcgag gaggcggtaa ctttctggat aaactcagcc
     3541 ataagagagc gagagttttg acatcaaatg acgtggatgg ttaggatcca tttgcaccgg
     3601 agcaagtcac cgcacatggg agtcaccggc atgcgtcgct tgggagtttt ggccgtcatt
     3661 gtttctggat tgcttgcagc gacacccact tcccgtgcag atcgactgga cgagatcatt
     3721 gctgcccaga atcaggcggc cggactgaag ctggccgcga cgccgatcgt tgacgatgag
     3781 gtgtttttgc ggcgcgtgtt cgtcgatctg atcgggcgga ttccgaccac cggggaagtc
     3841 aacgctttca ccgccgccga ccccaaggac cgccgcgaaa agctggtcga gaagctgctg
     3901 gccgacgagc ggttcgccga ccgctggacg attttctatg ccgacatgtt gcggctgcgg
     3961 tcgaa
//
LOCUS       DASUGB010000108         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1402204, whole genome shotgun sequence.
ACCESSION   DASUGB010000108 DASUGB010000000
VERSION     DASUGB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402204"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1309)
                     /locus_tag="VFV87_02040"
     CDS             complement(2..1309)
                     /locus_tag="VFV87_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5102561.1"
                     /translation="MSDREGPNPYQSPAEGEPALPAAASLWPLFILFGSLYFVQGVVE
                     PTAGMIFQPIQSQLQDWNYSPASISAMFAFIGIPWSLKLLFGLVSDFFPVFGRRRRPY
                     LVFSTVAAATLFAGISFGWGDPAVVGSYAWVLMLICGAVAMTDVVIDALAVETGQPLR
                     ITGQLQSVQWGAMSAASILGGLLGGVIAGYRVLGPALLGCGLLCGLSLIVVLIVVREP
                     RRQHVPVENLRLAFQALTTRGRFLILLSAGIYLFLWNFNPFSSNVLLHYSTEVLGFSD
                     PFYGALNSIQAAAQAVACAGYFLVCRRIPFGWLIHGSILAGILSTLCYWPMRDATTAI
                     FASIAFGLTYQIATLIQLDLAARIAPTEAAGTIFALLMAISNTGITASTWVSGAWYDW
                     LASAEGLGSRHAAFDSLVAIGAAFTAACWLVVPVMKWAGVEWR"
     gene            1640..3001
                     /locus_tag="VFV87_02045"
     CDS             1640..3001
                     /locus_tag="VFV87_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX5102562.1"
                     /translation="MNSSDIEQSTATVDLEEPPGSNLTSSASYNLPLYSPPGGTVAPP
                     SFDGEPKATDLGDGRSQGTAGPAVGSPLNERRRRAASTRTRAFRRAYFCDVPDKDWND
                     WRWQSRHRIRKLDQIERMLVLSDDEREALIKGESMLPVGITPYYMSLLDRENPSQPLR
                     RTVIPVTGEFLRTPGEADDPLGEDGHSPTPGLVHRYPDRVLLLALDFCSTYCRYCTRS
                     RVVGHGEIMPSEARLEKCFDYIRSTPEIRDVLISGGDPLALSEDKLDWILGNLRAIPH
                     LEFIRIGTKMPAVLPQRITPALIRVFKKYHPLWMSVHFLHPDECTPEAAIACNRLADA
                     GIPLGSQTVLLKGVNDSVPVMKDLCHRLLTMRIRPYYIYQCDPISGSAHFRTSVSKGL
                     EIIEGLRGHTTGYAVPTYVIDAPGGGGKIPLQPNYIVGRDGDDLLLRNYEGQVYRYPD
                     PAG"
     gene            3085..3618
                     /locus_tag="VFV87_02050"
     CDS             3085..3618
                     /locus_tag="VFV87_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102563.1"
                     /translation="MSREEPLCVRCARHQKNCCQVPQILVTLGDVRRIEAETGQTEFY
                     EFRLPDDPAYADQDDDPIWRDNAFQPDGTRRVLKKQANGDCAFLGPRGCVLSIDARPL
                     VCRLYPFDFTADGILDEPAPGCPLELLHPGQQLIPTLGMSRLEAQRWRAQLYEELQLE
                     GAKKTGERTALDKDSFG"
     gene            3688..3840
                     /locus_tag="VFV87_02055"
     CDS             3688..3840
                     /locus_tag="VFV87_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102564.1"
                     /translation="MIRKLKSGQYRLYSRKVNPQTGKRRNLGTFETREQAEKHERAVQ
                     YFKRQH"
     gene            4153..5289
                     /locus_tag="VFV87_02060"
     CDS             4153..5289
                     /locus_tag="VFV87_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5102565.1"
                     /translation="MDRRSTWSKWVTPFVALGSVAVVSGAMALAQVSDESETPAKNKA
                     KSPPAEREAASQPDRAAPRRSPDRSADSELPPDQPRRPAAAPDDRPQPANARRPEDRA
                     RPQDQGADETRRGEGDRANACADLGIEFNDQAEGGLTVQKIDQDSVAAQSGLRNGDVI
                     VSVDGRSIRNPRQFQAYLSGQYGRRVPVIIDRGGQRYTVQFSMGEPSGDAAWLGVYLQ
                     EGEEGQQGAQITQVYPAGPAARAGMRTGDVIVRVNDQAIESTPDLISTVESLDPQSQA
                     QFVVLRGDREVPLNVRLGRRDQFIYYGGQDDYGGGGDRSGYSDSDDDDFNNIPPYAMQ
                     LEHDRRVAEQHQRIEQELFQLREEVRKLREALQPNRPGRPAPQE"
     gene            5341..5691
                     /locus_tag="VFV87_02065"
     CDS             5341..5691
                     /locus_tag="VFV87_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102566.1"
                     /translation="MNAFARTILLSLAAALLLMIACPAPTQANDDDYWDNYWGWYDGT
                     YRPYYTRHYYSYSPGYSYGYYGPGNYYGGYNDGWYGSRYYGGRYYGTPRFGYRDYPYG
                     GGSVRVGPLRFGWR"
     gene            6031..>6221
                     /locus_tag="VFV87_02070"
     CDS             6031..>6221
                     /locus_tag="VFV87_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102567.1"
                     /translation="MLIELGLASGLLGAEPTIRIDTPMLPPAWALLERELLRANAAAC
                     EEFFARYFDERGFLQCVER"
BASE COUNT         1220 a         2016 c         1937 g         1048 t
ORIGIN      
        1 attaccgcca ttcgactccc gcccacttca tcaccggcac caccagccag caggcggcag
       61 tgaaggcggc gccgatggcg accagggagt cgaatgcggc gtggcggctg cccagtccct
      121 cggcactggc cagccagtcg taccaggcgc cgctgaccca ggtcgaagcg gtgatccccg
      181 tattactgat cgccatcagc agggcgaaga tcgttccggc ggcttcggtg ggggcgatgc
      241 gggcggcgag gtcgagctgg atcagcgtgg cgatttgata ggtcaggccg aaggcgatgc
      301 tggcgaaaat tgccgtcgtc gcgtcccgca tgggccagta gcacagcgtg ctcaggatgc
      361 cggccaagat cgagccgtgg atcagccacc caaacggaat ccgccgacag acgaggaaat
      421 atccagcgca ggcgacggcc tgagccgcgg cttggatcga gttgagcgcg ccgtagaacg
      481 ggtcgctgaa gccgagaact tcggtcgaat agtgcaggag cacattgctg ctgaaggggt
      541 tgaagttcca gaggaacagg tagatccctg ccgaaagcaa aatcaggaat cggccgcggg
      601 tcgtcaatgc ctggaatgcc agtcgcaagt tctcgaccgg cacatgctgc cgccgcggct
      661 cgcggacgac gatcaggacc acaatcagcg acagcccgca cagcaaaccg cagccgagga
      721 gcgccggccc gagcacgcgg taacccgcaa tcacgccgcc tagcaagccg ccgagaatcg
      781 acgccgccga catcgccccc cattggaccg attgaagctg gcccgtgatt ctcagcggct
      841 ggccggtttc gaccgccagg gcgtcgatca ccacgtccgt catcgccacc gcgccgcaga
      901 tcagcatgag cacccaggca tacgagccga cgaccgccgg atcaccccag ccaaaagaaa
      961 ttcccgcaaa aagcgtcgcc gcggcaacgg tcgaaaacac caggtacggc cgccgccgcc
     1021 ggccaaacac ggggaagaaa tcggaaacca gtccgaacaa cagtttcagc gaccacggaa
     1081 tgccgataaa ggcgaacatg gcggatattg atgcgggcga atagttccaa tcctgtagtt
     1141 ggctctggat gggttgaaag atcatgcccg cggtcggctc gacgacgcct tggacgaagt
     1201 agagcgagcc aaacaggatg aacaggggcc acagacttgc cgcggctggt aaggcgggct
     1261 cgccctcggc cggcgattgg tacggattcg gaccctcgcg gtcgctcatg gagacgattt
     1321 tccagattct ggccaatcct gaaagattga ccattgacag cacccgcggg tggcccttta
     1381 attccgcgtc aatacacctg cgcacgctgc gtggagccgg gaatggacgc acaagctgct
     1441 aagcgccgaa atcgggggcc aggcacggcc cgcgtcggcc ggggcatgcc tgcgtcccgc
     1501 gtctggtcgc ctttcttcgc gacaaattct gcacccgctg ttcacgcctc cggccgccat
     1561 gttggcggct gctcgctgcc cgtcaaggag accggtgcgg cgtcggttgc gcaaattggc
     1621 tcctggggac ttccccttta tgaacagctc ggacatcgag caatcgactg ccacggtaga
     1681 tctggaagag cctcctggta gtaatctcac atcttccgcc tcttacaact tgccacttta
     1741 ttcgccgcct ggcggcaccg tcgcgcctcc gtcgtttgac ggggagccga aggcgaccga
     1801 cctcggcgac ggccgctcgc agggtaccgc agggcccgcc gttggctcgc cgttaaacga
     1861 gcgtcgccgc cgggccgcca gcacccgcac gcgggccttt cgccgggcct atttctgcga
     1921 cgtacccgat aaggactgga acgattggcg ctggcagtcg cggcatcgca tccgcaagct
     1981 cgaccagatc gagcggatgc tggtcctctc ggacgacgag cgcgaggcgc tcatcaaggg
     2041 cgagtcgatg ctgccggtcg gcatcacccc ctattacatg agcctcctcg accgtgagaa
     2101 tccgtcgcag cctctgcggc gaacggtcat tcccgtcaca ggcgaattcc tccgcacgcc
     2161 gggcgaagcc gacgatccgc tcggcgagga cggccacagc ccgacgccgg gcctggtgca
     2221 tcgctatccg gaccgcgttc tcttgctcgc gctcgacttc tgctcgacct actgccgcta
     2281 ctgcacgcgg tcgcgtgtcg tcgggcacgg cgagatcatg ccgagcgaag cccgcctgga
     2341 gaagtgcttc gactatattc gcagcacgcc ggaaatccgc gatgtactga tcagcggcgg
     2401 cgacccgctc gccttgtcgg aagacaagct cgactggatt ctcggcaact tgcgggcgat
     2461 tccccacctg gaattcatcc gcatcggcac caagatgcca gccgtcctgc cgcagcggat
     2521 cacgccggct ttgattcggg tcttcaagaa gtaccatccg ctctggatga gcgtccactt
     2581 cctgcacccg gatgaatgca cgccggaagc cgcgattgcc tgcaatcgcc tggccgacgc
     2641 ggggattccg ctcggatcgc agacggtgct attgaagggc gtcaacgaca gcgtgccggt
     2701 gatgaaggat ctgtgccacc gcctgctgac gatgcggatc cggccgtact acatctacca
     2761 gtgcgacccg atcagcggca gcgcccattt ccggacgagc gtcagcaagg ggctggaaat
     2821 catcgaaggt ctccgcggcc ataccacggg ctacgcggtg ccgacctacg tgatcgacgc
     2881 ccccggcggc ggcggcaaga ttccgctgca gcctaactac atcgtcggcc gcgacggcga
     2941 cgacctgctc ctgcggaact atgaagggca ggtctatcgc tacccggacc cggctggcta
     3001 gccgccgcgc ccatcaatcg aggttccaat aggatgggct gtgccgatcc gtttcacgtg
     3061 atggcgggta caacttgacc tactatgtcg cgcgaagagc cactctgcgt ccgctgcgcc
     3121 cggcatcaga aaaactgctg ccaggtgccg cagattctcg tgacgctcgg cgatgtgcgg
     3181 cggatcgagg cagaaacggg acagacggaa ttctacgagt tccgcctgcc ggacgacccg
     3241 gcttatgcgg accaggacga cgatccgatc tggcgcgaca atgcctttca gccggatggc
     3301 acgcggcggg tcctcaagaa acaggccaac ggcgattgcg cgtttctcgg cccgcgcggc
     3361 tgcgtactgt cgatcgacgc acggccgctc gtttgccggc tctatccatt cgatttcacg
     3421 gcggatggca ttctcgacga acccgctccc ggctgcccgc tagaattgct gcaccctggt
     3481 cagcaactga ttcccacgct cggcatgagc cgcttagaag cccaacggtg gcgagcccag
     3541 ctctacgagg aattgcagct cgagggcgca aaaaagaccg gcgagcgcac cgctttagat
     3601 aaagactcct tcggctgacc cggtatatgg cacgcgagtt gcggcctttt cgactgcagg
     3661 tcagttcgtt cttccgaagc attcgccatg atccgcaagc tcaaatccgg tcagtaccgc
     3721 ctctactctc gcaaagtcaa tccgcaaacc ggcaagcgga ggaatctcgg cacgttcgag
     3781 acgcgcgagc aagccgaaaa gcacgaacgc gccgtgcaat actttaagcg ccagcactga
     3841 ccccagcgcg gtaggcgttg attagcaatg tccgaatgct ttccggaagc atcggcagcg
     3901 aagacgtcga ttttgtccgg cttcgccgac atcctgcgtc cgacgtgtcg cgacattgcc
     3961 gctgcgctgt acccaggccc cgggcaaacg gagcttggat gtcaccctcc agcgtttgct
     4021 ttggcgactt gtcgtgcttg cgccgctctg ccccgcgtat ccgctgctcg gcacaaaagc
     4081 tgctgagttt attcgcggtg ccggcatcga ccggcgatca atgcgccaac cgcaagcaca
     4141 aggagaacgg gaatggaccg tagatcgacc tggagcaaat gggtgactcc gtttgtggca
     4201 ttgggaagcg tcgccgtggt gagcggtgca atggcgctgg cccaggtcag cgacgaatct
     4261 gaaacgccgg cgaagaataa ggcgaaatcg ccgcccgccg agcgcgaagc agccagccaa
     4321 cccgatcggg cggctccgcg gcgctcgccg gatcgcagcg ccgacagcga gttgccaccg
     4381 gatcaacctc gccgaccggc tgcggccccg gatgatcgac cccaaccggc aaacgcccgg
     4441 cgacccgaag atcgggcccg gccccaggac cagggagcag acgagacgcg gcgcggcgag
     4501 ggggatcggg ccaatgcctg cgccgacctc ggcattgaat tcaacgacca ggcggaaggg
     4561 ggcctgacgg tccagaagat cgaccaggac agcgttgcgg cccaatccgg cttgcggaac
     4621 ggcgacgtca tcgtctcggt cgatggacgc tccattcgca atccacgcca gttccaggct
     4681 tatctaagcg ggcaatacgg ccgccgcgtc ccggtcatca tcgaccgagg aggccagcga
     4741 tacaccgtgc agttctcgat gggcgagccc agcggcgacg ccgcctggct gggcgtctat
     4801 ctgcaagagg gcgaagaagg ccaacaggga gcccagatca cgcaggtcta tccggcgggt
     4861 cctgcggccc gggccggaat gcgcacgggc gatgtcattg tgcgtgtgaa cgatcaggcc
     4921 atcgaaagca cgccggacct gatctccacc gtcgagtcgc tcgatcccca atcgcaggcc
     4981 cagttcgtcg ttctgcgtgg agaccgcgag gtgcccctga acgtcaggct cggccgccgg
     5041 gatcaattca tctactacgg tggccaggac gactatggcg ggggcggcga tcgcagcggc
     5101 tacagcgaca gcgacgacga cgatttcaac aacatcccgc cgtacgccat gcaactcgag
     5161 cacgaccgcc gggtggccga gcagcatcag cggatcgaac aggagttgtt ccagctccgc
     5221 gaggaagtcc gcaaactccg cgaggccctt cagccgaacc ggcctgggcg acctgcgcca
     5281 caggaatagc ggctggccgt ttttgcaacc acatcaccaa acagactcgg agattcgaac
     5341 atgaatgctt tcgcacgcac gatcctgcta tctcttgcgg cggcgctctt gctgatgatc
     5401 gcctgtcccg cgccgaccca agccaacgac gacgattact gggacaacta ctggggctgg
     5461 tacgacggaa cgtatcgacc ctactacacg cgacactact acagctatag ccccggatat
     5521 agctatggct attacgggcc cggcaactac tacgggggat acaacgacgg gtggtacggc
     5581 agccgttact acggcggccg ctattacggc acgccgcggt tcggttaccg ggattatccg
     5641 tacggcggtg gatcggtccg cgtcggtccg ctccgtttcg gctggcggtg acgatcgcgg
     5701 attgactcgc ttctgtccat catcgtagca gccattgaag agagccggcg agctttatgc
     5761 acgtgccgcg acgaagatca ggtgcgtcag gctgtcgagg ctgccgccaa tcctggcctc
     5821 acccgctgac tttccgcacc ttctgcagcg ggagttccgg cgtcgattgc cgccggccag
     5881 gaatcgcggc gacgcgacag gcgcggcacg tgccaatatt cgctgtacgg ccctccccgc
     5941 cgcaagtaag ctggtgccca gcaccggttc tactgcacgg ggggagggct gcggatgcaa
     6001 cggtcgcgac cgattgtcgt tttcttaggg atgctgatcg aactgggcct ggcatccggt
     6061 ctgttaggcg ccgagccgac aatccgcatc gatacgccca tgttaccccc cgcgtgggcg
     6121 ctgcttgagc gagaactgtt gcgggcgaat gccgcggcgt gcgaggagtt ctttgcccgc
     6181 tattttgatg agcggggctt cctgcaatgc gtcgagcgct g
//
LOCUS       DASUGB010000109         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1819640, whole genome shotgun sequence.
ACCESSION   DASUGB010000109 DASUGB010000000
VERSION     DASUGB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819640"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFV87_02075"
     CDS             <1..783
                     /locus_tag="VFV87_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102568.1"
                     /translation="VPGQSYYHVLLGQVLLKGPDGYSQGIAEFEEANRLSPDKSYFAY
                     RLGNAYARWGRWREAAPLLRRASDTWPTDRWNAYHHAILSAYVGDMESYRLACRRLLD
                     ADKPDGADLGPYNAAIACLLAPDGIGQSDRIRELAIEEQAERLNDRRKANARALAQLR
                     AGQWRSAIDELGTVPTEPNGARNHLTSLAILAMAQQGSGDLPAAKQSLATAKSLRAAT
                     WPYPEQGRPFGEFWQNWLLAEILLREAEGLIAPPAQAQQAPP"
     gene            961..1824
                     /locus_tag="VFV87_02080"
     CDS             961..1824
                     /locus_tag="VFV87_02080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5102569.1"
                     /translation="MFLRRQVLLLSAAAIALAGSANPAKAESPFSKLYVFGDSLSDTS
                     NVFLATGGEGAGFPYYEGRFSNGPVRVEVLAQALDLPAPEASLLGGSNYAWGAAETGP
                     GLSFWGTFNVGMQIESFLADHGGFAGDELIVVEAGSNDLLWSSPYGAGHIVENLRKHI
                     ADLASAGGRTFLIANSPVGGSDGAKLNQLLAQELPKLEQKLGVSIIPFDLAGVYQVIR
                     QNPGIFGLTNITDPACPGCGIGIPEPDAIDTLVPNPDEYFKWDQIHPTRVVHAIMGQA
                     AAEVVWAEVLP"
     gene            1858..2523
                     /locus_tag="VFV87_02085"
     CDS             1858..2523
                     /locus_tag="VFV87_02085"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5102570.1"
                     /translation="MSTLIVILNGVLLLALLVLGFLVLGTLRALGVVNWRIDQLQATT
                     PSRIGRDGLRIGKQAPDFTLPGQERHDVSLHEFQGRKVLLVFTQAGCGPCHDIVPELN
                     RLHDRGECQVVGINNGELGEAAIFAAETGARFPVLAQEKFSLSKRYQVFATPFAFFID
                     EQGVIRSKGTVAMRQHLGYVLSGIGQTGQVHGGDAEPEASPRPLSADTMSPLANSLET
                     TLA"
BASE COUNT          503 a          857 c          810 g          498 t
ORIGIN      
        1 gtaccagggc aaagttatta tcacgtattg ctcggtcaag tgctgctgaa agggccggac
       61 ggctattccc aaggcattgc agaattcgaa gaggccaatc gcctcagtcc cgacaagtct
      121 tacttcgcct accggctcgg caatgcctat gcccgctggg ggcggtggcg agaggccgcc
      181 ccattactcc gccgcgcctc cgacacgtgg ccgaccgata ggtggaacgc ataccatcat
      241 gcgatcttgt ctgcgtatgt cggcgacatg gaaagctacc gcctggcctg ccgccggctg
      301 ctcgacgccg acaaaccgga cggcgctgac ctaggaccct acaacgccgc gatcgcatgc
      361 ctgctcgcgc ctgatggaat tggccagtcg gatcgcattc gcgagttggc gattgaggaa
      421 caagccgaga ggctcaatga tcggcgcaag gcgaacgcgc gagcactggc gcagctcagg
      481 gctggccagt ggcgctccgc catcgacgag ctcggaaccg ttccgaccga accgaacgga
      541 gcgcgcaacc acttgacctc gctggccatc ctggcgatgg cgcagcaagg ctcaggagac
      601 ttgcctgccg caaagcaatc gctggccacc gcgaagtcgc tgcgcgcggc aacgtggccc
      661 tatcccgagc agggacgccc gttcggcgaa ttctggcaaa attggctgct ggccgaaatc
      721 ctcttgcggg aagccgaagg gctcatcgcg ccaccggccc aggcacaaca ggccccgccc
      781 taaggagtac caatgagcac gtaactcttc agaatcgttc gaaacaaaac ggccggcgaa
      841 gactagccgc taagcacgtt cctcgccggc cgccagttgc cgccgccagc gcacggtctg
      901 cgcgccgggg gatcggcttt ttcattatac cgcaggccgc aattgcagga gaccgattcc
      961 atgtttctgc gacggcaagt tctgctgctt tccgcggccg cgattgcact ggcgggcagc
     1021 gccaatccgg ccaaggccga gtccccattt tcgaaactat atgtgttcgg cgacagcctc
     1081 tcggacacga gcaacgtctt cctggccacc ggcggcgagg gagccggctt cccgtattac
     1141 gaggggcgat tctccaacgg ccccgtccgg gtggaagtgc tggcccaggc actcgatctg
     1201 ccggcgccag aggccagcct gctgggaggc agcaactacg cttggggagc cgccgagacc
     1261 ggccccggct tatcgttctg ggggacgttt aacgtgggga tgcagatcga gtccttcctg
     1321 gccgatcacg gtggttttgc cggcgacgag ctgattgtcg tcgaggccgg cagcaacgat
     1381 ctcctctggt cttcccccta tggcgccggc cacatcgtgg aaaacctccg caaacacatt
     1441 gccgatctgg cgagcgcggg agggcgaacg tttctcattg caaattcgcc cgtcggcggg
     1501 tcggacggtg cgaagctaaa ccagttgctc gctcaagaac ttcccaaact cgaacagaag
     1561 ctgggcgttt cgatcatccc gttcgatctg gcaggagtgt accaggtgat ccggcagaat
     1621 cccggcatct tcggcctgac gaacatcacc gatcctgcct gcccaggctg cggcatcggt
     1681 attcctgaac ctgacgcgat cgacacgctc gtccccaatc cagacgagta cttcaagtgg
     1741 gaccaaatcc atccgacgcg cgtggtccac gccatcatgg gccaggccgc tgccgaggtc
     1801 gtttgggcgg aagtcctgcc ctgatcgcct tgccctttcc tatgggagga atatcacgtg
     1861 agtaccctga ttgtcattct caatggggtc ctgctgcttg cactgttggt gcttggtttt
     1921 ctcgtgctcg gcacgctgcg agcgctggga gttgtgaact ggcggatcga tcagttgcag
     1981 gcgacgacgc ccagtcgcat cggccgtgac ggcctgcgga tcggcaaaca ggccccggat
     2041 ttcacgctgc caggccagga aaggcacgac gtctcgctgc acgaatttca gggacgcaag
     2101 gtgctcctgg tattcacgca ggccggctgc ggcccgtgtc acgacatcgt tcccgagctg
     2161 aatcggttgc acgaccgggg cgagtgccag gtggtcggca tcaacaacgg cgagttgggc
     2221 gaagccgcga tctttgcggc ggagacgggc gcccgctttc cagtcctggc gcaggagaag
     2281 ttcagcctct ccaagcgcta ccaggttttc gccacaccct tcgcattttt cattgatgaa
     2341 cagggggtca ttcgctcgaa aggcacggtc gcgatgcggc agcacctcgg gtacgtgctt
     2401 tccggcattg gtcaaaccgg tcaggtccac ggcggcgatg ccgaacccga ggcatcgccg
     2461 cgcccccttt ctgcggacac gatgtccccg ctagcgaact cgttggaaac gaccctcgct
     2521 tgaacagcgc aatcacgccg ccgccagcgc ggcctccgtc gttgatcgtc aatccgtttc
     2581 gcgtcccttt cacaggagtc gatccatgtt tgagagattc agtcacctgg ccgaacaagc
     2641 agcgcacgac gcttcccgcc ggcagttt
//
LOCUS       DASUGB010000110         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1959053, whole genome shotgun sequence.
ACCESSION   DASUGB010000110 DASUGB010000000
VERSION     DASUGB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959053"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFV87_02090"
     CDS             <1..620
                     /locus_tag="VFV87_02090"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX5102571.1"
                     /translation="GWPVPLHRTFEEVNLRFYVRRVTSGEVRRGVSFIKEIVPRWAIA
                     QTARWCYNEPYIALPMRHEITGPLGSLHAGPVATRRDDSSSRSEMPADSPGCIRYEWR
                     FANRWNSLSLNYCGAAAPLALGSLEEFIAEHYWGYCPQRDGGTVEYQVEHPPWHVWQG
                     AEPQFDCDIAPLYTPRFAELLCRPPSTAFLADGSAVAVMRPLRIA"
     gene            715..1323
                     /locus_tag="VFV87_02095"
     CDS             715..1323
                     /locus_tag="VFV87_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5102572.1"
                     /translation="MLGASLDLSAYLERLNREVSRLNQADIQKWADLTYDAWQQGRQV
                     FIFGNGGSATTASHFAEDLGKSTLRPEDLKDESKKRLKVLSLTDNVGWLLAVGNDCGY
                     DQIFVQQLMNYGNAGDLVIAISGSGNSPNVLNAVDWANRHGLVTFGLTGYKGGKLKAL
                     QQHGLHVDLDDMGMVESIHLCVFHWVLNDVYARINYDGRYAT"
     gene            complement(1311..2186)
                     /gene="folD"
                     /locus_tag="VFV87_02100"
     CDS             complement(1311..2186)
                     /gene="folD"
                     /locus_tag="VFV87_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5102573.1"
                     /translation="MSKILDGKALATAMEAELAEQVADFIQNHAYAPCLSAVLVGDDP
                     ASAIYVKNKRLACERVGIESQLHRLGPAANEEELLELIARLNNDEEVSGILVQLPLPV
                     QIREQKVLDAVHPLKDVDCFHPENVGLMVQGRPRFLPCTPHGCLQILHRHHLPVAGKH
                     AVVVGRSEIVGKPLATLLAQKESHLGPSACNATVTLCHSKTANLAEVCRQADVLVAAI
                     GRPKFITAEMVKPGAVVLDVGINRTESGLVGDVDFEAVQMIASAITPVPRGIGPMTIT
                     MLMHNTLAAARGQVA"
     gene            2550..>3318
                     /locus_tag="VFV87_02105"
     CDS             2550..>3318
                     /locus_tag="VFV87_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102574.1"
                     /translation="MPKTTFFAKTSLLILVAIFFLVPFAIRGARFAIQGMKNDVKDWL
                     PKDFEETQDLDEFRRYFLSEQFVLVSWEGCFGDETDERFKLFTAKLTPETPPSALPPE
                     DPGAIFRPAEAAPAELAAEEPADEPSRNPTLYIHHDNDFIGDRLGLYFAGDWHEDWGR
                     RGEKWLRGQRLKHEGSNEESWYYITPEGDLYRWDGVDAPHAALYREIRRALTDEQVEG
                     TLVHSFGPVDGPWYYAQPRRLRAQLFKTVTTGPAVLDS"
BASE COUNT          623 a         1072 c         1021 g          602 t
ORIGIN      
        1 tgggctggcc ggtgcccttg caccgcactt tcgaggaagt gaatctgcgg ttctacgtgc
       61 gccgcgtcac tagtggcgaa gtccgccgcg gcgtttcgtt tatcaaggag atcgtgccga
      121 ggtgggccat cgcgcaaacc gcccgctggt gctacaacga gccctacatc gccctgccga
      181 tgcgccacga gatcaccggc ccgcttggta gtcttcatgc tgggccagtc gccaccaggc
      241 gcgatgacag cagctcgcgc agcgagatgc ccgccgactc gcccggatgc atccgctacg
      301 agtggcgttt cgccaaccgc tggaattcgc tctcgctcaa ctactgcggc gcggcggccc
      361 cgctggctct cggctcgctc gaagaattca tcgccgaaca ctactggggc tattgcccgc
      421 agcgggacgg cgggaccgtc gagtaccagg tcgagcatcc gccgtggcac gtttggcaag
      481 gcgccgagcc gcaattcgac tgcgatatcg cccccctcta cacgccccgg ttcgccgagc
      541 tgctctgccg gccgccgtcc accgcgttcc tcgccgacgg atcggcggtc gcggtaatga
      601 ggcccctgcg aattgcttag ccggcaacct ctatacgcgg cttttggtac tcagtacata
      661 gaaggaacac ggaactaatg actcgcccca atccctaagc cctggcctga cacgatgctc
      721 ggcgcctctc tcgatctatc tgcctacctc gaacgcttga accgcgaagt gtcgcggctg
      781 aatcaggcgg atattcagaa atgggccgac ctgacgtatg acgcctggca gcaggggcgg
      841 caggtgttca tcttcggcaa cggcggcagc gcgacgacgg cttcgcactt tgccgaagac
      901 ctgggcaaga gcacgctgcg gccggaggac ctgaaagacg aatccaaaaa gcgactgaag
      961 gtgctgagcc tgaccgacaa cgtcggctgg ctgctcgcgg tgggcaacga ttgcgggtac
     1021 gaccagattt tcgtccagca gcttatgaac tacggcaatg cgggcgatct cgtcatcgcc
     1081 atcagcggca gcggcaacag ccccaacgtc ctgaatgccg tcgattgggc caatcggcac
     1141 gggctcgtga cgttcggcct caccggctac aaggggggca agctaaaggc cctgcaacag
     1201 catggcctgc acgtcgatct cgatgacatg gggatggtcg agagcatcca cctgtgcgtg
     1261 tttcactggg tgctgaacga cgtgtatgcg cggatcaatt acgacggacg ctatgcgacc
     1321 tgaccccgcg cggcggccag cgtgttgtgc atcagcatcg taatcgtcat cggcccgatg
     1381 ccgcggggca cgggcgtgat ggcgctggcg atcatctgaa cggcttcgaa atcgacgtcg
     1441 ccgacgaggc cgctttcggt gcggttgatg ccgacgtcga gcacgacagc accgggcttg
     1501 accatctccg ccgtgatgaa cttcggccgg ccgatcgcgg cgacgagcac atccgcttgc
     1561 cggcagacct cggccaggtt ggctgtcttg ctgtggcaga gcgtcaccgt ggcattgcac
     1621 gcggacggcc ccaggtgcga ttccttctgg gctaaaagcg ttgccagcgg cttgccgacg
     1681 atctcgctgc ggccaacgac caccgcgtgc ttgcccgcga ccggcagatg gtggcgatgg
     1741 agaatctgca ggcagccgtg cggcgtacag ggcaagaacc gcggccggcc ctggaccatc
     1801 aaaccgacgt tttccgggtg aaagcaatcg acgtccttca gcggatggac tgcgtcgagc
     1861 accttctgct cgcgaatctg caccggcagc ggaagctgga cgaggatgcc gctcacttcc
     1921 tcgtcgttgt tgagccgggc gatcagctcc agcagctcct cttcgtttgc cgccggtccc
     1981 aggcgatgca actggctttc gatgcccacc cgctcgcacg ccaggcgctt gtttttcaca
     2041 tagatcgcgc tagccgggtc gtccccgacc agcacggcgg aaaggcaggg ggcgtacgca
     2101 tggttctgga tgaaatcggc cacctgctcg gccaattccg cctccatcgc cgtcgccagg
     2161 gccttgccgt cgagaatctt actcatcgcc aaccttcaca acctattccc gcgaaaacgc
     2221 gcaacgcttg cctgacccgc aaagtcaacg ccagcatttc agccacaagt ccaatcagca
     2281 tatcagacag caggctgctg aattgcctgt atgcagctag atcgtcgtcg aacggctctc
     2341 aaagatcgcg gaggacggat ttgtcgcttc aagctgcttt gcgcattttg ccgaaggtga
     2401 tgatatcgcc gtgcgcaatt gtgcttggac ctggctggac cgtaacccga agcgtcagcg
     2461 cgggagtccc tcgctaacac ttcgagttac tgtcgcacag cctttcgcgg agcgaaaggc
     2521 gacgaaccct cttcagcgac ggatcgcaga tgcccaagac gacgtttttc gccaagacca
     2581 gcctgctgat actggtggcc atctttttcc tcgtgccgtt cgccattcgc ggcgcgcggt
     2641 tcgccatcca ggggatgaag aacgacgtca aggactggct gccaaaggac tttgaggaaa
     2701 cgcaggacct ggacgagttc cgccgctatt tcctcagcga gcaattcgtg ctggtgagct
     2761 gggaaggctg ctttggcgac gagaccgacg aacggttcaa gctcttcacg gccaagctga
     2821 cgccggaaac gcccccatcg gctctgccgc cggaagatcc cggggcgatc tttcgccctg
     2881 ccgaggccgc gccggcggaa cttgccgccg aagaaccggc tgacgagcca tcccgaaatc
     2941 ccacgctcta catccatcac gacaacgact tcatcggcga ccgtctgggt ctgtactttg
     3001 ccggcgactg gcacgaggac tggggccgcc gcggcgagaa atggctgcga gggcagcggc
     3061 tcaagcacga agggagcaac gaggagagct ggtattacat cacgccggaa ggcgacctgt
     3121 accgctggga cggcgtcgat gccccgcacg ccgctcttta ccgggaaatc cgccgggcct
     3181 tgaccgacga gcaggtcgaa gggacgctgg tccactcgtt cggcccagtc gatggcccct
     3241 ggtattacgc ccagccgcgg cgcttgcggg cgcagctctt caagacggtg acgaccggcc
     3301 cggcggtgct ggacagcc
//
LOCUS       DASUGB010000111         6914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1542342, whole genome shotgun sequence.
ACCESSION   DASUGB010000111 DASUGB010000000
VERSION     DASUGB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542342"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFV87_02110"
     CDS             complement(<1..716)
                     /locus_tag="VFV87_02110"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5102575.1"
                     /translation="MSRARPFLVVAAIGAISASAFAEEEGFVPLFNGKDLSGWVNVNL
                     APDTFTVKDGILVSTGKPTGTIRTERMFENFILELEWRHMVAGGNAGVFIWGDPVTAV
                     GTPFNRGIEVQVLDGRETENYTSHGDVFAIHGATMTPDRPHPAGWARCLPSEKRAKPS
                     PEWNHYRITCNDGKLKLAVNGKEVSGGYACSPRKGYICLESEGSECHFRNLKIKELPS
                     TQPTAEETASEALGFMPLYTG"
     gene            874..1341
                     /gene="ribH"
                     /locus_tag="VFV87_02115"
     CDS             874..1341
                     /gene="ribH"
                     /locus_tag="VFV87_02115"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5102576.1"
                     /translation="MPNVLQGDSGPVSGRFAVIVSRYNEHITGKLLAGAVETLQAAGV
                     AEGEIDVAWVPGAWEIPLVAQRFAQSEEYVAVLCLGAVIKGETTHDEHINRQVSLSLG
                     QIALATDVPVLFGVLTCNTVEQAIHRAGGNVGNKGVECAQAALEMVRLMEQLP"
     gene            1338..3128
                     /locus_tag="VFV87_02120"
     CDS             1338..3128
                     /locus_tag="VFV87_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102577.1"
                     /translation="MTKLARCLFFQLLLSFCSQASIAQQPPAEKSGDKTNIFRQPEEL
                     PATAEPRLVEKNVALVGPENTAWDAKASLFRPAFRFADDDQRKSAEAVTGHGAAGELV
                     RQWQMEGTAAGLWGVLYDNRDRDHSNLPIQEFPQLTGVEYSDEAKQAGLDMGLQLRTL
                     FAQPTFGNSSTAATNGPFWRSQTRIGQVDFQRVDRQYQQYVNNHLYLYPEHRDYDPGH
                     NGKDSGYGDTYPANTPYVITSQGSSYTDQPFLHAVACTLAGFQPATRDKLVESKLLIP
                     AVQMIFRRSNKQVATDEDYLTGKAHPPVFQGEQLDLERMVRLAHEIQPDEIPPMIQLQ
                     VVKEDEFRRGIHYFEPVPTEKLFDTPAAIARVWRSTARERKIVVTAATSYDANNRPLK
                     FHWAVLQGRPERIDIQPLKEDGSLVEITLQWHERFPIAPPSETERSKMESNRIDIGAF
                     AHNGSHYSAPGFVTFYCLDDETRKYNAAGQPEVIEYKSLAKGGNYADPAVHTLRDFTD
                     EYHYDETGELTGWTRIRGAEREFFTAHGHLLVEKDSLGRATKARPVIYVVKDFQPGKA
                     PYLEQIAEDVILVYEYATDADRVGKAVLLP"
     gene            3137..3319
                     /locus_tag="VFV87_02125"
     CDS             3137..3319
                     /locus_tag="VFV87_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102578.1"
                     /translation="MRSMQFTSRDLIWITIAVALALGWAGDHRTQKWALKKASERAVH
                     LMLENEQLRTQIERPK"
     gene            3379..3873
                     /locus_tag="VFV87_02130"
     CDS             3379..3873
                     /locus_tag="VFV87_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102579.1"
                     /translation="MKIASVLGILASLCLIGGAQADIRVPPEKREPVRITSKVSIKHG
                     VIRGAGRDVAAKIIIPASLVHPAEPVPPPNKQGALPGNLGTMIAGIALSLAAVSLVFV
                     IRGRRTTRTAAATILGGAVLLGAFGAVYANAPAPNRPEPPVAQQIVIELVEEGDSVTL
                     LLAK"
     gene            3874..4899
                     /locus_tag="VFV87_02135"
     CDS             3874..4899
                     /locus_tag="VFV87_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102580.1"
                     /translation="MRASVAAELDTCSAREISPSRSAWLFAGAAAGVIGLSCALAGFA
                     PLGFSIAAVFLFAGPHNWLEARYMLTRMPARWGPLAPYFLTGIGGVLLLTGSFALLPW
                     IGGGLSGSGWNNLLATWNTLLIGWIVTLALLRSRQNPRRDWTWLVPIGLTLVALNWLW
                     PFALSLALVYLHPLLALWFLDREIGRMQPAWRTAYRGCLLLVPACLALLWGNLAYSPN
                     LPGDDVLTSQITSHAGGGILNGISTHLLVATHVFLEMLHYGVWIVAIPLISVRMAPWK
                     LDNVPLARRSGFWRTAVIGAIATGAIVMLLLWGAFLADYPLTRNVYFTVAMLHVLAEV
                     PFLLRLL"
     gene            4984..5379
                     /locus_tag="VFV87_02140"
     CDS             4984..5379
                     /locus_tag="VFV87_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102581.1"
                     /translation="MPPDAAHLWSFLPWGYALTVALELPVLLVGLSPRHPISRRVFAG
                     FWLTASTYPIVVLVLPPLVWNRWGETAYLIVAETFAPAAECLLFWLLFCREVPPPPAS
                     GTLRDLAAIAIANLVSFLVGGWLVERLLA"
     gene            complement(5535..5945)
                     /locus_tag="VFV87_02145"
     CDS             complement(5535..5945)
                     /locus_tag="VFV87_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HSP20 family small heat-shock protein"
                     /protein_id="HEX5102582.1"
                     /translation="MLPKSAEPLQREMDQMFEHFFGNGGQASAAFFAPASLWEEENCW
                     KVEVDLPGVKQEDVDLTIEKNSLRITAERKGPSDDRKFWHQERAYGRIDRLITLPETV
                     DAEGITAELREGVLHLTLSKRPESQPRKIQVKAK"
     gene            complement(6242..6424)
                     /locus_tag="VFV87_02150"
     CDS             complement(6242..6424)
                     /locus_tag="VFV87_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEX5102583.1"
                     /translation="MSDEASYLCDWCGEEIVVPIDLSQGERQEYVEDCPVCCRPNVIG
                     AEADEDGTLRLWARRE"
     gene            6561..>6914
                     /locus_tag="VFV87_02155"
     CDS             6561..>6914
                     /locus_tag="VFV87_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX5102584.1"
                     /translation="MRGVLISANLLASVALVLAFAGSAAAARSEEATVQAAGDVLQEF
                     LDLQIKNIPASLLADARGVAIIPDLIKLGFVLGGQRGKGVVMIREADGTWRAPTFITL
                     TGGSIGWQVGAQSSDI"
BASE COUNT         1196 a         2192 c         2211 g         1315 t
ORIGIN      
        1 cccgtataga gcggcataaa gccgagggcc tcgctggcgg tttcttccgc ggtgggctgt
       61 gtcgatggca actccttgat cttgagattt cggaagtggc actccgagcc ttccgactcc
      121 aggcagatat agcccttccg cgggctgcac gcgtagccgc cgctgacttc cttgccgttg
      181 accgcgagct tcagcttgcc gtcattgcag gtgatgcggt agtgattcca ctcgggcgac
      241 ggtttggccc gcttctcgct gggcaagcac cgggcccagc cggcagggtg cgggcggtcg
      301 ggggtcatcg tcgcgccgtg gattgcgaac acgtcgccat ggctggtgta gttctccgtc
      361 tcgcggccat cgagcacctg gacctcgatg ccgcggttga acggcgtgcc gaccgccgtc
      421 accggatcgc cccagataaa cacgcccgca ttgccgccgg cgaccatgtg ccgccactcc
      481 agctccagga tgaaattctc gaacatccgc tccgtgcgga tcgtccccgt cggcttgccg
      541 gtgctgacga ggatcccgtc tttcaccgtg aacgtgtccg gcgcgaggtt gacgttcacc
      601 cagccggaaa gatccttgcc attgaagagc ggaacgaagc cttcctcctc ggcaaatgcc
      661 gatgcggaga tggccccgat ggcagcgaca actaaaaatg ggcgggcacg tgacatggcg
      721 aggttcctgg tggtcttgcg gctggtcgtg cctcgaatct accagcggcg gcgctgcgat
      781 tccacttgtt gcagcgcgag gggtcgggcc cacgccagcc ggattgcttc tcgcggagcg
      841 agaagcctac gatgggcgaa cggaggtgtt tcgatgccca acgttctgca aggcgattct
      901 ggtccggttt ccggccggtt cgctgttatc gtttctcgat ataacgagca catcacgggc
      961 aagctgctgg ccggggcggt cgagacgctg caggcggcgg gcgtggcgga aggggagatc
     1021 gacgtggcct gggtgccggg ggcatgggag attccgctgg tggcccagcg gttcgcccaa
     1081 tcggaggaat atgttgccgt gctgtgcctc ggggcggtga tcaagggcga aacgacacac
     1141 gacgagcaca tcaaccggca ggtgagcctg agcttggggc agatcgctct ggcgaccgat
     1201 gtgccggtgc tgtttggcgt gctcacctgc aacacggtcg agcaggcgat ccaccgcgcc
     1261 ggcggcaacg tggggaacaa gggggtcgaa tgtgcccagg ccgcgctgga aatggtccgc
     1321 ctcatggagc aactgccgtg acgaaactcg cgcgctgcct tttcttccag ctcttgctgt
     1381 ccttctgcag ccaggcatcg attgcccaac aaccgccggc tgagaagtcc ggggacaaaa
     1441 ccaatatctt ccggcagccg gaagaattgc ccgccaccgc cgagccgcgg ctggtcgaga
     1501 agaatgttgc gctcgtcggc ccggagaata ccgcgtggga tgccaaggcg agtctattcc
     1561 gcccggcatt ccgctttgcg gacgacgatc aacggaagtc ggctgaggcg gtgacagggc
     1621 atggcgctgc gggcgagttg gttcgccagt ggcagatgga gggcacggct gccggcctgt
     1681 ggggcgtgct gtacgacaac cgcgaccgcg accacagcaa tctgccgatc caggagttcc
     1741 cgcagctcac cggcgtcgaa tatagcgatg aggcgaagca ggccggcctc gatatgggcc
     1801 tgcagctccg cacgctgttt gcccagccga cgtttggtaa ttcatccact gcggcaacaa
     1861 acgggccgtt ttggcgaagc cagactcgaa tcgggcaggt cgattttcag cgcgtcgatc
     1921 gacagtatca gcagtacgtc aacaaccacc tgtacctcta ccccgagcac cgcgactacg
     1981 acccggggca caatggcaag gacagtggct atggcgacac ctatccggcg aatacgccat
     2041 acgtcatcac cagccagggc tcctcgtaca ccgatcagcc gtttctgcac gcggtcgcct
     2101 gcaccttggc cggcttccag ccggcgacgc gtgacaagct ggtcgagtcg aagctgctga
     2161 tacccgcggt gcagatgatt tttcgccggt cgaacaagca ggtcgcaacg gatgaggact
     2221 atctgaccgg caaggcccat ccgccggtct ttcagggcga gcagctcgac ctcgagcgga
     2281 tggtccgcct ggcccacgag atccagccgg acgaaattcc gccgatgatt cagttgcagg
     2341 tcgtcaagga ggacgaattc cgccgcggaa ttcactattt tgagccggtg ccgaccgaga
     2401 agctgttcga cacgcccgcg gcgattgccc gcgtgtggcg ctcgaccgcc cgcgagcgaa
     2461 agatcgtcgt caccgcggca accagctacg acgcgaacaa ccgcccgctc aagttccatt
     2521 gggccgtgct gcaagggcgg ccggagcgga tcgacatcca gccgctcaag gaggacggct
     2581 cgctggtcga gatcacgctc cagtggcacg agcggtttcc gatcgcgccg ccctcggaaa
     2641 cagagcgatc gaaaatggaa agcaaccgca tcgacatcgg ggcgttcgcc cacaatggct
     2701 cgcactactc ggcccccggc tttgtcacgt tttattgcct ggacgacgag acgcgaaagt
     2761 acaacgccgc cggccagccg gaggtgatcg agtacaaatc actggcaaag gggggcaact
     2821 atgccgatcc ggcggtccac acgcttcgcg atttcacgga cgagtaccac tacgacgaaa
     2881 cgggcgaact gaccggctgg actcgaattc gcggcgcgga gcgggaattc ttcaccgccc
     2941 acgggcatct actggtcgag aaggactcgc tcggccgggc gacgaaggcc cggcccgtga
     3001 tttacgtcgt caaggacttc cagccaggga aagcgccata tctggagcaa atcgcggagg
     3061 acgtgatcct cgtctatgaa tatgcgacgg acgccgatcg cgtggggaag gcggttttgt
     3121 tgccctaggt agtgccatgc gatccatgca gttcacgtcg cgcgacctga tctggataac
     3181 catcgctgtg gcgttggcgc tcggttgggc aggtgaccat cgcacacaga aatgggccct
     3241 gaagaaggcc tcggaacgag cagttcacct catgctggag aatgaacaac ttcgaactca
     3301 gatcgaacgt ccgaagtagc catttcacaa ccgccgatga ttcaagaatt ccgtccatcc
     3361 gaaatcaagg agcccgttat gaagattgcc tctgtcctgg ggatcctggc gtcactttgt
     3421 ctgataggcg gagcgcaggc cgatatccgc gtgccgcccg agaagcggga accggtgcga
     3481 attacgtcaa aggtgtcgat caagcacggg gtgatccgcg gcgcgggtcg tgacgtggcc
     3541 gccaagatca tcattccggc gagcctggtg catcctgccg agcccgtccc gccgccgaac
     3601 aagcaaggcg cgctgccggg caatttagga acaatgattg ccggcatcgc cctctcgctg
     3661 gcggccgtaa gtctggtctt cgtcatccgc ggcaggcgga ccacgcggac ggcggcggcg
     3721 acgatcctgg gcggggcggt tttgcttggt gcgtttggcg cggtgtacgc caacgcgcct
     3781 gctccgaatc ggcccgagcc gccagtggca cagcaaatcg tgatcgagct ggttgaggaa
     3841 ggcgattccg tcacgctgct gctggctaaa taaatgaggg catctgtcgc tgctgaactc
     3901 gatacatgtt ccgcgcgcga gatttcccca tcccgcagcg cctggctgtt tgccggcgcg
     3961 gcggcgggtg tgatcgggct gtcgtgcgcg ctggccggct ttgcgccgct ggggttttcg
     4021 atcgcggcgg tgttcctctt cgcggggccg cacaactggc tggaagcccg ctacatgctc
     4081 acccgcatgc cggcccgttg ggggccgctc gcgccgtatt tcctcaccgg gatcggcggc
     4141 gtgctgctcc tcaccggcag cttcgccctg ctgccgtgga ttggcggcgg gctatcgggc
     4201 agcggctgga ataacctgct ggcgacgtgg aacacgctgc tgatcggctg gatcgtgacg
     4261 ctggctctgc tgcgcagccg gcagaatccg cggcgcgatt ggacctggct ggtgccgatc
     4321 ggcctgacgc tcgtcgccct caactggttg tggcccttcg ccctcagcct cgccctcgtc
     4381 tatctgcacc cgctgctggc cctgtggttc ctcgatcgcg agatcggccg catgcagccg
     4441 gcgtggcgca ccgcctaccg cggctgcttg ctgctggtgc cggcgtgtct cgcgctgctg
     4501 tgggggaacc tggcctattc gccaaatctg ccgggcgacg acgtgctgac gtcgcaaatc
     4561 accagtcacg ccggcggcgg cattttgaac ggcatttcga cccatctgct cgtagcgacg
     4621 cacgtcttcc tggagatgct gcactacggc gtctggatcg tggccattcc gctgatcagc
     4681 gtgcggatgg ccccgtggaa gctcgataac gtgccgctgg cccgccgctc gggtttctgg
     4741 cggacggcgg tgatcggagc gattgcgacc ggcgcgatcg tgatgctcct cctgtggggc
     4801 gcgttcctgg ccgactatcc gctgacgcga aacgtctatt tcaccgttgc catgctgcac
     4861 gtcctggcgg aggtgccgtt cctgctgcgg ttactgtagg ttccgacagc cgcggcggaa
     4921 ccagactcgc caatccacgt tgtttcctgt cgctggtccc gcccaggcgg tcgggaccta
     4981 cttatgccgc ccgatgccgc gcacctctgg tcatttctac cctggggcta tgccctcacg
     5041 gtggcgctgg aactgccagt gctgctcgtg ggcctctcgc cgcggcatcc gatctcgcgg
     5101 cgcgtcttcg cggggttctg gctcaccgcc agcacctatc cgatcgtcgt cctcgtcctg
     5161 ccgccgctgg tgtggaaccg ctggggcgaa acagcctacc tcatcgtcgc cgagaccttc
     5221 gctcccgcag ccgagtgcct gctgttctgg ctgctgttct gtcgcgaggt tccgcctccg
     5281 cccgcgtcag gcactttgcg cgacctggcg gcgattgcga tcgccaacct cgtttcgttc
     5341 ctggtgggcg gctggcttgt cgagcggctg ctggcctagc ctgacactaa cccgaagcgt
     5401 tagcgaggga gtccctcgct atcgcttcgg gttacagtgc cgctggcaag aagcgaaaaa
     5461 actccccggc tgcaatcaca accgaggtgc aaggtctttc cgctactgtc cgctcgcggg
     5521 gcgagcagac cactttattt cgccttgacc tgaatcttcc gcggctgcga ttcgggccgc
     5581 ttgctgagcg tcaggtgaag gacgccctcg cgcagctcgg cggtgatgcc ctcggcatcg
     5641 accgtctcgg ggagcgtgat cagccggtcg atccggccgt aggcccgctc ctggtgccag
     5701 aacttgcggt cgtccgaggg ccccttccgc tcggccgtga tccgcagcga gttcttctcg
     5761 atcgtcagat cgacgtcctc ctgcttcacg ccaggcagat cgacttcgac cttccagcag
     5821 ttctcctctt cccacagcga agccggggcg aagaacgccg ccgaagcctg tccgccattg
     5881 ccaaaaaagt gctcgaacat ctggtccatc tcgcgctgca gaggctcggc cgatttcggg
     5941 agcacgaacg accaacgggg tttcagcgtt ccaaccatat gcgattctcc tatttcgggt
     6001 gaaggaaacg ggaaagtgtg tcgtgagtta ctcttgaaga ctttccgcca actttggcag
     6061 ccggcggagg acgggaagac aagaaagcaa cgagcgtgcc aattgcaaat tggcagcctg
     6121 cccgtgagtt cccgccccca gacaacgcgg ccgccgccgg cgatgccgga attgccggtt
     6181 tcgacgggag atgggctgcc aaaatgacaa gcccgcgggc agcggccctg gaggttccga
     6241 cctattcgcg ccgggcccaa agccgcagcg tgccatcctc gtcggcctcg gcgccaatca
     6301 cattcgggcg gcagcagacc gggcaatcct cgacatactc ctgtcgctca ccttgtgaaa
     6361 gatcgatcgg cacgacgatt tcctcgccgc accaatcgca cagatagctg gcttcgtcag
     6421 acatttgagc attgtagtag cctgccggct cgcggtggga gcatcgccgc tagcagcgcc
     6481 caccttgaat tgtggttgcc gctgtcaggg gagtcgcgat acagttcctc tcgatccgtc
     6541 ctcggcacag gagtccggcc atgcgcggcg tcttgatatc tgccaatctg ctcgcctcgg
     6601 tagcgttggt tctggcattc gccggaagcg cagcggccgc ccgcagcgaa gaagcgaccg
     6661 tgcaggccgc tggcgatgtg ttgcaggaat tcctcgactt gcagatcaag aatattcctg
     6721 cctcgctgct cgccgatgcc cgcggcgtgg cgatcattcc ggacctgatc aagctcggct
     6781 tcgtgctggg cggccagcgc ggcaaagggg tcgtgatgat ccgcgaagcc gacggaacct
     6841 ggcgggctcc cacgttcatc acgctcaccg gcggcagcat cggctggcag gtgggagcgc
     6901 aatcgagcga tatt
//
LOCUS       DASUGB010000112         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_430073, whole genome shotgun sequence.
ACCESSION   DASUGB010000112 DASUGB010000000
VERSION     DASUGB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430073"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFV87_02160"
     CDS             complement(<1..667)
                     /locus_tag="VFV87_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102585.1"
                     /translation="MNGGEHELDEEPRAVKPRRIDGPHATGLKMADRSTVAAQELVAA
                     SAGESATSLAWSGDGGDALEGMLELEESQGSREEQLQQQAAQLADQLQAQLRELESRE
                     SYQNARAAELDNDFRRARLWISEKSAELQERETAVAAAEARLAAARAEAERQLTLSEE
                     SAASEAAQMLRRSEAGLREVEARQEVLLQSEQVLAHRQQIFAHREEELSERDRQLESQ
                     EFAL"
     gene            914..1201
                     /locus_tag="VFV87_02165"
     CDS             914..1201
                     /locus_tag="VFV87_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5102586.1"
                     /translation="MKVTPIGDKVVVRRLDAEEKTPGGIVLPDAAQKKPQQGRVLSVG
                     DGRLIAGGRRVPNQVQEGDKVVFLTYAGTEVSVDDEELLILREDDILAVID"
     gene            1264..>2000
                     /locus_tag="VFV87_02170"
     CDS             1264..>2000
                     /locus_tag="VFV87_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102587.1"
                     /translation="MSTRRDFLTITAAFGAASALLPRLVWGEDKSPEQLVTKAIGYLT
                     TKGQAGDGSYTATSGPGITGLVVAGILRNGRSPDDPAVAKSLKYLEGFIQPDGGIYSK
                     ESFHKNYETCICVLAFTEANKNGRYDKVIKGADAYIKGIQWDESEGKAKDDVFYGGAG
                     YGRSARPDLSNTTFLMDALTATGNDENSDAIKRALIFVSRCQNLETEHNTTPFAAKNP
                     DGGFYYTPAGTSGNSQAGIDEKTGGLR"
BASE COUNT          388 a          610 c          625 g          377 t
ORIGIN      
        1 ggagggcgaa ttcctggctt tcgagctggc ggtcccgctc cgacagttct tcctcgcggt
       61 gcgcgaaaat ctgctggcga tgggccagta cctgctcgct ctggaggaga acttcctgcc
      121 gggcttccac ctcgcgaaga cccgcctcgc tccgtctgag catctgggcc gcctcgctgg
      181 cggctgattc ttcgctgagt gtcaattgcc gctcggcttc cgcacgcgcc gccgccagac
      241 gagcttcggc agcggcgacg gcggtttccc gctcttgcag ctcggcggac ttttcggaga
      301 tccagaggcg ggcccggcgg aaatcgttgt ccagctcggc agcccgcgcg ttctgatacg
      361 actcacgact ttccaactcg cgcagctgag cttgcagttg atcggccagt tgagccgcct
      421 gctgctggag ctgttcttcg cgagagccct gggattcctc gagctcgagc attccctcca
      481 gcgcgtcgcc cccgtcgccg gaccacgcga gactagtcgc ggattcgccg gcgctcgcgg
      541 caacaagctc ctgagccgct acggtggagc gatccgccat tttcaggccg gtggcgtgcg
      601 ggccgtcgat ccggcggggc ttcacggccc gcggctcctc gtccaattcg tgttcaccgc
      661 cattcatgtc gccactcctt tggcaacgag acttactgcg ccagcaggcg gcagtttcca
      721 cgcaaccggc aaggtcaccc tattttgacg cctcttacat cggcagattt ctgttcggaa
      781 gttagcgcgt ttgagttagg attgaagtga cagcattgct tgcacccacc ttggccgcat
      841 gggacaatct cgcgtcgccg ggacgctccg atttacgggg ggattgccgc gacgaaacgg
      901 ggaaatcagc aacatgaaag ttactcccat tggggacaag gtcgtggtca ggcgtttgga
      961 tgcggaggaa aagacacccg gcgggatagt gctccccgat gccgcacaaa agaagcctca
     1021 gcaagggcgg gtgctatcgg tgggggacgg gcggctaatt gcgggggggc ggcgagtccc
     1081 caatcaggtg caggaagggg ataaggtggt cttcctgacc tacgctggca cggaagtttc
     1141 tgtggatgac gaagaactgc tcattttgcg ggaagacgac atcctggcgg tgatcgacta
     1201 gccgaaggtg gcgatttttt ggccgcaccg ggcaggaagc actgcaattc tttggagatg
     1261 gacatgtcga ccaggcgcga ttttcttact atcaccgctg cttttggtgc cgcctcggcc
     1321 ctgctgccgc ggctggtttg gggtgaagac aaatctcccg aacagttggt gaccaaggcc
     1381 atcggctacc tgacgaccaa ggggcaggcc ggcgatggtt cctacaccgc cacgagcgga
     1441 ccgggcatca cggggctcgt ggtcgccggt attctgagga acggacgctc ccccgacgac
     1501 ccggccgtgg ccaagagcct caagtatctg gaaggcttca ttcagccgga cggcggcatc
     1561 tattccaagg aatcgttcca caagaattac gagacctgca tctgcgttct ggcctttacc
     1621 gaagccaaca agaacggccg gtacgacaag gtcatcaagg gagcggacgc ctacatcaaa
     1681 ggaattcagt gggacgaatc cgagggaaag gccaaggacg acgtcttcta cggcggagca
     1741 ggctacggcc ggtcagcccg ccccgacctc tccaacacga cgtttctgat ggacgccctt
     1801 acggcgactg gcaacgatga aaatagcgac gccatcaagc gagctctgat cttcgtgtcc
     1861 cgctgccaga acctggaaac cgagcacaac acgacgccgt tcgcagccaa aaatccggac
     1921 ggcggctttt actacacacc ggcgggaacc tccggcaaca gccaggcggg aatcgacgag
     1981 aagaccggcg gactgcggag
//
LOCUS       DASUGB010000113         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_430238, whole genome shotgun sequence.
ACCESSION   DASUGB010000113 DASUGB010000000
VERSION     DASUGB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430238"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VFV87_02175"
     CDS             <1..2020
                     /locus_tag="VFV87_02175"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5102588.1"
                     /translation="VQVLQADLVHLRSGMTREWTEFPETAAGEKLEIKFQATTNERQW
                     ALQLRQQDVKQNWRVTVNDQALGTLVIDENDQTLYFLVPPGTLADGENVLRIAAARTG
                     AASDDIRVGQVRLHPRPVADVLVEGSVEIQVTDADSHQPLPCRLTILNAEGSLQTTAA
                     ASSDELAVRPGVIYAARGRARLGVPLGKYTIYAGRGFEYSLAKAEVAVGRGPAVPLMM
                     EIRREVPTEGYVACDTHVHTVTFSGHGDATIAERMITLAGEGIELPIATDHNVHVDYR
                     SHIAKAGLSEYFTPVMGNEVTTSVGHFNVFPIQLDAEPPDHKSKEWSAIFDGIFGTPG
                     VKVAVLNHARDVHGGTRPFGPKLHNAVIGENLEGWPLRFNAMEVVNSGATQTDVLQLV
                     HDWMGLLNAGRLITPVGASDSHDVARHFVGQGRTYIRAGDRNPGEIDVEQAVNNFLQG
                     RVLVSYGLMAEMTVDSKYGSGELALPSGDGMSVVVRVRGPSWVKADQVMLFSNGRLVR
                     HESIPTIETTRADGVKWESTYKLPPLKHDSYLVAVALGPGIDGPWWKTAKPYQPTSPD
                     WEAHLIGCSGAIRLDGDGDGRWSSPRDYAQQAVAASGGDLKELVQSLGGYDEATAAQA
                     AHLYQQTGKSLVAPESSEILKTAPEHVQRGFADYFKAWRESELARAEP"
     gene            2136..2522
                     /locus_tag="VFV87_02180"
     CDS             2136..2522
                     /locus_tag="VFV87_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5102589.1"
                     /translation="MSRKLPADLSRRERQIMEIIYVRGEASAAQVLEQLPDPPSYSAV
                     RALLSILVQKGQLKHRAEQGRYVYAPTRRRARAGRSALVRVLETFYEGSLEKAVAALL
                     TPDSKLSADELKRLSELIEQARKEGR"
     gene            2525..>2855
                     /locus_tag="VFV87_02185"
     CDS             2525..>2855
                     /locus_tag="VFV87_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102590.1"
                     /translation="MTTTLVDAGLKGLVLLAAIALVALAMRRASAARRHLVWTLAVIG
                     LLVLPVLSAALPAWRVTMLPAWLAESAAESRVPVLNTKVEPAPAQRAIAEAPPAVEWP
                     PAPIAQRA"
BASE COUNT          514 a          893 c          979 g          469 t
ORIGIN      
        1 ggtgcaagtg ctgcaagccg atctcgtcca cctgcgcagc ggcatgacgc gggaatggac
       61 ggaattccca gaaacggcgg cgggcgagaa gctggagatc aagttccagg ccaccacaaa
      121 cgagcggcag tgggcgctgc aactccggca gcaggatgtc aagcagaatt ggcgggtaac
      181 ggtcaacgac caagcgctcg gcacactcgt gatcgacgag aacgaccaga cgctctactt
      241 tctcgtgccg ccggggacgc tggctgacgg cgagaatgtt ttgcggatcg cggcggcccg
      301 aacgggggcg gcgtccgacg atattcgcgt cgggcaggtc cgcctgcacc cgcggccggt
      361 cgcggacgtg ctcgtcgaag gcagcgtcga gattcaagtc accgacgccg actcccacca
      421 gccactgccg tgccggctga cgattttgaa tgccgagggc tcgctgcaga cgacggccgc
      481 ggccagcagc gatgagctgg cggtgcggcc gggagtgatc tatgcagcgc ggggccgggc
      541 aaggctcggc gtgccgctgg gtaagtacac catttatgcc ggccgcgggt tcgagtattc
      601 gctggccaag gcggaggtcg ccgtcgggcg aggcccggct gtgcctttaa tgatggaaat
      661 ccgccgcgaa gtgccgacgg agggttacgt cgcctgcgat acgcacgtcc atacggtcac
      721 gttctccggc cacggcgacg cgacgatcgc cgagcggatg atcacgctgg ccggcgaagg
      781 gatcgagctg ccgatcgcga ccgatcacaa cgtccacgtc gattaccgtt cgcacatcgc
      841 caaagcgggc ctgtccgagt atttcacgcc ggtgatgggg aacgaagtta caacgagcgt
      901 cggccacttc aacgtgttcc ccatccagct ggacgccgag ccgccggatc acaagtcgaa
      961 ggagtggtcg gcgatcttcg atggcatctt cggcacgccc ggcgtgaagg tcgcggttct
     1021 caaccacgcc cgcgacgtgc atggcggcac gcggccgttc gggccgaagc tgcacaacgc
     1081 cgtgatcggc gagaatctcg aaggctggcc gctgcgattt aatgcgatgg aggtcgtcaa
     1141 ctccggcgcg acgcaaaccg atgtgctgca gctcgttcac gactggatgg gactgctcaa
     1201 tgccggccgc ctgatcacgc cggtcggcgc cagcgattcg cacgacgtgg cccggcattt
     1261 cgtcggccaa gggcggacgt acatccgcgc aggtgaccgc aatccgggag aaatcgacgt
     1321 cgagcaggcg gtcaacaact ttctgcaggg ccgcgtcctg gtgagctatg gcctgatggc
     1381 cgaaatgaca gtcgatagca aatacggctc gggcgaactg gcgctgccgt cgggcgacgg
     1441 gatgtcggtc gtcgttcgcg tgcgcgggcc gagttgggtg aaggcggacc aggtgatgct
     1501 cttcagcaac ggccggctgg tacgccacga gtcgattccg acgattgaaa ccacgcgcgc
     1561 tgacggcgtg aaatgggaga gcacatacaa gctgccgccg ctgaagcacg attcgtacct
     1621 ggtggccgtt gccctcggcc cgggaattga cggaccgtgg tggaagacgg ccaagcccta
     1681 tcagccgacg tcgcccgatt gggaggccca cctgattggt tgcagcgggg cgattcggct
     1741 ggacggcgac ggcgacggcc gctggtcgag cccgcgcgac tatgcccagc aggcggttgc
     1801 ggcatcgggc ggcgatctaa aagaacttgt ccagagcctg ggcggctacg acgaagcgac
     1861 cgctgcccaa gccgcccacc tttatcaaca aacgggcaaa tcgctcgtgg cgcccgaatc
     1921 gtctgagatt ctgaagaccg cgccggagca cgtgcagcgg ggtttcgccg attacttcaa
     1981 ggcctggcgg gagtcggagt tggcgcgggc cgagccgtag ggtgtataag tctgcctcgg
     2041 ctgcacgcgc gggccagagg cccacgccac gatctgctaa ttttttagca tctgggactt
     2101 gactcggacc gcctgatgct aatattttag cacctatgag ccgcaaactg cctgccgact
     2161 tgagccgccg tgagcggcag atcatggaga tcatttacgt ccgcggcgaa gcctccgccg
     2221 cccaggtgct ggagcagttg cccgatccgc cgagctattc ggccgtgcgg gccctgctct
     2281 cgatcctggt acagaaggga cagctcaagc accgggccga gcagggccgg tatgtctacg
     2341 ccccgacccg ccgccgcgcc cgggccggcc gctcggcgct cgtccgcgtg ctggaaacct
     2401 tttacgaagg ctcgctggag aaggcggtcg ccgcgctgct cacgcccgac tccaagctct
     2461 cggcggacga gctgaagcgg ctcagtgaac tgatcgaaca agcccgcaaa gagggacgct
     2521 agccatgacg acgacattgg ttgatgccgg tttgaaaggc ctggtcctgc tcgcggcgat
     2581 cgccctcgtc gcactggcga tgcggcgagc ctcggctgcc cggcggcatc tcgtctggac
     2641 gctggctgtg atcggcctct tggtgctgcc ggtgctctcg gcggcgttgc ctgcgtggcg
     2701 cgtgacgatg ctgccggcgt ggctggcgga atcggcggcg gagtctcgag ttcctgtgct
     2761 gaataccaaa gtggaaccag caccggccca gcgggcgatt gccgaagcgc caccggccgt
     2821 cgaatggccg ccggcgccaa tcgcgcagcg cgccc
//
LOCUS       DASUGB010000114         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1820105, whole genome shotgun sequence.
ACCESSION   DASUGB010000114 DASUGB010000000
VERSION     DASUGB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820105"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1740
                     /locus_tag="VFV87_02190"
     CDS             202..1740
                     /locus_tag="VFV87_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5102591.1"
                     /translation="MNHQPILILGGGINGAAAARELTLNGAGVWLVDTADLSFGATAY
                     SSRLIHGGLRYLEFAEFSLVKESLDERARLLRLAPHLVKPLRLFIPVRNSWGGFTQAA
                     GKFLGLPLRFKRTAHRGLRVVQVGLWLYDRYASGGDLPPRSLHEPIEPGVPRVSQAVA
                     THLWAYSDAQITYPERLVIALLDDARKLAQQHGSEFRVFTYSRATLHGSTTEIRPTGD
                     DAAAPLATLKPAAIINATGAWVDRTLARLPVESRRLMGGTKGSHFLTYHRPLREALAG
                     QGVYTEASDGRPVFLLPFGEGTLVGTTDIPFEGDPATAVATEQELDYLVSVATEVFPD
                     VGLTRGDIKMHQCGVRPLPYADAKSTAAITRRHQLVWSDHAPVPLVSLVGGKLTTCRS
                     LAEETTAAVLDRLGKRVEQTSRERPIPRLQVDEALAAGIQNWRSIAASSIQHEWATRL
                     EDLVERRLMLHFQPRISRNLLGDLAAALVQCGHLDLAEAESAINRCTDRLKTHFGVNL
                     TPDS"
     gene            1754..>2082
                     /locus_tag="VFV87_02195"
     CDS             1754..>2082
                     /locus_tag="VFV87_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5102592.1"
                     /translation="MSYILALDQGTTSSRSIIFGKHGRIVATAQQEFPQILPQPGHVE
                     HDPEAIWQTQLDTAKAALADAKLSAADIAAIGITNQRETTILWEKATGKPVANAIVWQ
                     SRISTPI"
BASE COUNT          361 a          742 c          634 g          345 t
ORIGIN      
        1 acggcttgcg atatgctact ccgcacgtcg gcgcgtcgca accaatgcga ttgcgcgggc
       61 cgaaaagatc ggcaagtcgt tccggtcgca acgcctttgc cgaatgtgcg gagagcggct
      121 tcttgttggt gctggtgtgc gtgacccgcc gcacgcgtta cgatgcgcgt gaggcctagc
      181 tcgcgcggca catcaatcgc gatgaatcac cagccgatcc tcatcctcgg cggcggaatc
      241 aacggcgccg ccgctgcgcg cgagctgacg ttgaacggcg cgggtgtgtg gctcgtcgat
      301 acggctgacc tctcgttcgg agcaacggcg tattcatcgc ggctcattca tggtggcctg
      361 cggtacctgg agttcgccga gttctcgctc gtcaaggaat cgctcgacga acgcgcgcgg
      421 ctgcttcggc tcgccccgca tctggtaaag cctctgcggc tcttcattcc ggtgcggaac
      481 agttggggcg gatttacgca ggcggccggc aagttcctgg gcctgccact gcggttcaag
      541 cgaacggctc atcgcggcct gcgtgtcgta caggtgggcc tgtggctgta cgaccgctat
      601 gccagcggcg gagacctgcc gccgcgatcg ctgcacgagc caattgagcc cggcgtgccg
      661 cgcgtcagcc aggcggtcgc cacccacctg tgggcctatt ccgacgccca gatcacatat
      721 cccgagcggc tggtcatcgc tctgctggac gatgcgcgga agctcgccca acagcacggc
      781 agcgaattcc gcgtcttcac gtacagccgg gctacgctgc acggcagcac gaccgagatt
      841 cgcccaactg gcgacgatgc cgctgccccg ctcgccacac tcaagccggc cgccatcatc
      901 aacgcgaccg gcgcctgggt cgatcgcacg ctggcccgcc tgccggtcga atcgcgccgc
      961 ttgatgggcg gcacgaaggg gagccacttt ctgacgtacc atcggccgct gcgcgaggcg
     1021 ctcgccgggc aaggcgtcta taccgaagcc agcgacggcc ggccggtgtt tctcttgccc
     1081 ttcggcgaag ggacgctcgt cggcacgacc gacattccgt tcgagggcga tccggcaacc
     1141 gctgtggcta ccgagcagga gctcgactat ctcgtttccg tcgcgaccga ggtctttccc
     1201 gatgttggcc tgacgcgcgg cgacatcaag atgcaccaat gcggcgtgcg cccgcttccg
     1261 tacgccgatg cgaaatcgac cgccgcgatc acccgccgac atcagcttgt ctggagcgat
     1321 cacgctcccg tgccgctcgt gtcgctcgtc ggcggcaagc tcacgacgtg ccgctcgctg
     1381 gccgaggaaa cgactgccgc tgtattggac cgactcggca aaagagtgga gcagaccagc
     1441 cgcgagcggc cgattccgcg cctgcaggtc gatgaagccc tcgcggctgg aatccagaat
     1501 tggcgatcga tcgccgccag cagcattcag cacgaatggg cgacgcgcct cgaggacctc
     1561 gtcgagcggc ggctgatgct gcactttcag ccgcgaatct ctcgcaacct gctgggcgac
     1621 ctcgccgccg ccttggtcca atgcggccac ctcgacctcg ccgaagccga atccgccatc
     1681 aaccgctgca ccgaccgcct caaaacccac ttcggcgtca acctgacccc tgactcctga
     1741 ccccctgccg cccatgtcct acatcctcgc cctcgaccaa ggcaccacct ccagccgctc
     1801 aattatcttc ggcaagcatg gccgcatcgt cgccacggcc cagcaggagt tcccgcaaat
     1861 cctcccgcag ccggggcacg tcgagcacga cccggaagcc atctggcaga cgcagctcga
     1921 taccgccaag gcggccctcg ccgacgccaa gctctcggct gccgacatcg cggcgatcgg
     1981 catcacgaac cagcgcgaaa cgacgattct gtgggagaaa gcgaccggca aacccgtcgc
     2041 caacgcgatc gtctggcaaa gccgcatcag cacgccgatc tg
//
LOCUS       DASUGB010000115         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1959516, whole genome shotgun sequence.
ACCESSION   DASUGB010000115 DASUGB010000000
VERSION     DASUGB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959516"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="VFV87_02200"
     CDS             <1..1914
                     /locus_tag="VFV87_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102593.1"
                     /translation="LEQNLLAELLGDQVIADVALIGRDTFMKEGAAMGILFQAKSTDI
                     LKNDLSSQRSRAFAREKANGATSETVQIAGRDVSLVSTPDNRLRSFYVIDGNYHLVTT
                     SRDLVERFLAIREGAGSLGASPEFRFARSNMPLTRKDTIFVYFSAAFFQNLLSPQYQV
                     ELERRMKSVTDMELLQLARLAAHGENLRYESIDDLVEAGLLPRGFGRRPDGSGPILTD
                     GGMIDSRRGMRGSFLPIPDVKITGLTQAEWDRIDRLNLQYSQQWRRMDPVMVGIQRFA
                     LPEKEAGPNRERIVIDANVNPLDETKYGWLLSILGPPTKQMVTPAEGDVIMVQASVRG
                     GTLFPSVQPHYLFLGMQDIAPLVTVQPTGLLQWYNLLRSAPGYVGSVPRAGYLDVLPF
                     NLGGSVPDENGFSRLPLGLWRRQGGDFSVVSFDPQLLASVTPQLRVVEAETEAQIRAH
                     VGDLNSAKVKPWINGMYYQRAITASAGNTRFIHQLNQQLHVPMGQAKELAEDILDAKL
                     HCSLGGEYQLMEEIGGPQIWESTAWAKRDLSKIPEDFEAPILKWFHGLDMHLLKTGDE
                     ISVHAELDMQRKPSEPKIDLPFFNLFGGGQKALAPKPQPPGSEELPPPLPPVKTPPKA
                     DPLPRPLPSAREL"
     gene            2140..>2921
                     /locus_tag="VFV87_02205"
     CDS             2140..>2921
                     /locus_tag="VFV87_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102594.1"
                     /translation="MASVRVERMSAELGRLPHVCMCCGQPAASMAHHKFYYDPLWLAT
                     IGMLLPWYVRYHFASQSVVVPAPLCARHAGRLKLPTYLGYGFAASLLLAAPIMVLAVV
                     LSNGMTTAAGIVFFLVFLYVIAALVGLMAVRLATPRMTAYDYRSITFGSVSSGFAEAI
                     TGRPLPGMYAASAAAAPGAFPAPGPAGHVQYPNYGPAPPLTRQQWIFIGAVTGSLAFV
                     AFMMFFGVVYLRYRSGARHREEVARAQQWHNDQLAQFQEKQA"
BASE COUNT          473 a          917 c         1002 g          529 t
ORIGIN      
        1 ctggagcaga acctgctggc ggaactgctc ggcgaccagg tgattgccga cgtggctctg
       61 atcggtcgcg acaccttcat gaaggagggg gccgcgatgg gcatcctctt ccaggccaag
      121 agcaccgaca tcttgaagaa cgacctcagc agccagcgca gccgggcgtt tgccagggag
      181 aaggccaacg gcgcgacgag cgaaaccgtg cagatcgccg ggcgcgacgt gtcgctggtc
      241 tcgacgcccg acaaccggct gcggtcgttc tatgtgatcg acggcaatta ccacctggtg
      301 acgacctcgc gcgacctggt cgagcggttc ctggcgattc gcgaaggggc tggctcgctc
      361 ggggcttctc ccgagttccg cttcgcgcgg agcaacatgc cgctgacgcg gaaggacacg
      421 attttcgtct atttttcggc ggcctttttc cagaacctgc tcagcccgca gtatcaggtg
      481 gaattggagc ggcggatgaa atcggtcacc gatatggagc tgttgcagct cgcccgcctg
      541 gccgcgcatg gcgagaacct gcggtatgaa tcgatcgacg atctcgtgga ggccggcctg
      601 ctgccgcggg gcttcgggcg gcgaccggac ggcagcgggc cgattctcac cgacggcggc
      661 atgatcgatt cgcgccgcgg catgcggggt tctttcctgc cgattcccga cgtcaagatc
      721 accggcctga cgcaagccga atgggaccgc atcgaccggc tgaacctgca atattcgcag
      781 cagtggcggc ggatggaccc ggtgatggtc ggcatccagc ggttcgcctt gccggaaaaa
      841 gaggccgggc cgaaccgcga gcggatcgtc atcgacgcga acgtgaaccc gctcgacgag
      901 acgaagtacg gctggctgct gtcgatcctc ggcccgccga cgaagcagat ggtgacgccg
      961 gccgagggcg acgtgatcat ggtccaggcg tcggtccgcg gcggcacgct gttcccctcg
     1021 gtgcagccgc actatttgtt cctgggcatg caagacatcg cgccgctggt gacggtgcag
     1081 ccgacgggcc tcttgcagtg gtacaacctg ctgcgttcgg ccccggggta tgtcggctcg
     1141 gtcccgcggg ccgggtatct cgacgtgctg ccgttcaacc tgggcggctc ggtgcccgat
     1201 gagaacggct tctcgcgttt gccgctgggg ttgtggcggc ggcagggggg cgacttttcg
     1261 gtcgtgtcgt tcgatccgca gttgctggcg agcgtgacgc cgcagcttcg cgtggtcgaa
     1321 gccgagaccg aggcccagat tcgcgcgcac gtcggcgatc tcaactcggc caaggtcaag
     1381 ccgtggatca acggcatgta ttaccagcgg gcgatcacgg cgtcggccgg caacacgcgg
     1441 ttcattcatc agctcaatca gcaactgcac gtgccgatgg ggcaggcgaa ggagctggcc
     1501 gaggacattc tcgatgcgaa gctgcactgc tcgctggggg gcgagtatca gctcatggag
     1561 gagattggcg ggccgcagat ttgggaatcg accgcgtggg ctaagcgcga cctctccaag
     1621 attccggagg acttcgaagc gccgatcctc aagtggttcc acgggctgga tatgcacttg
     1681 ctgaagacgg gggacgagat ttcggttcat gccgagctcg acatgcagcg caagccgagc
     1741 gagccgaaga tcgacctgcc gttcttcaac ctgttcggcg gcgggcagaa ggcgctggcg
     1801 cccaagccgc agccgcccgg cagcgaagag ctgcctccgc cgctgccgcc cgtgaagacc
     1861 ccgcccaaag ccgatccgct cccgcggccg cttcctagcg cgcgagagct gtaggccaaa
     1921 gccgcgacca gcggtcgcgg ttccgaagtg tttgtgatga cacgctggaa ctggttccca
     1981 ggctccagcc tgggaacccg ccgccgcgag gctcctgcct cgcggcgggc ttccatcacc
     2041 agaaataatg gcgtttccgc cgcgcgatgg ctacaatcgc ggcgttcctg cgggggttcg
     2101 gtttcgtggc ctggtgccgt gctgcgtggg agggatgcaa tggcgagtgt ccgcgtcgaa
     2161 cgcatgtcgg ccgaactggg ccggctgccg cacgtctgca tgtgctgcgg ccagcccgcc
     2221 gcgagcatgg cgcaccacaa gttctactac gacccgctgt ggctggcgac gatcggcatg
     2281 ctgttgccgt ggtatgtgcg ctaccacttc gccagccagt cggtggtcgt gcccgcgccg
     2341 ctttgtgcgc ggcacgccgg gcggctcaag ctgccgacct atttgggcta tgggtttgca
     2401 gcgtcgctgc tgctcgcggc gccgatcatg gtgctggcag tcgtgctgag caacggcatg
     2461 accacggcgg cggggatcgt ctttttcttg gtatttctgt atgtgatcgc agcgctggtc
     2521 ggcttgatgg ccgtgcgact ggcgacgccc agaatgaccg cctacgacta ccgctcgatc
     2581 accttcggca gcgtgtcgtc cggctttgcg gaggcgatta ccgggcggcc gcttcccggg
     2641 atgtatgcgg cgagcgcggc cgccgccccc ggcgcatttc ccgcgccagg gccggcgggg
     2701 cacgtgcagt atccgaacta cggccccgcc ccgccgctta cgcggcagca gtggatcttc
     2761 attggggcgg tgactggatc gctcgcattc gtcgccttca tgatgttctt tggcgttgtc
     2821 tatttgcgct atcgcagcgg agcgcggcac cgcgaagaag tggcccgcgc acagcagtgg
     2881 cacaacgacc aactggcgca gttccaggaa aagcaggccg a
//
LOCUS       DASUGB010000116         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1543054, whole genome shotgun sequence.
ACCESSION   DASUGB010000116 DASUGB010000000
VERSION     DASUGB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543054"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFV87_02210"
     CDS             complement(<1..297)
                     /locus_tag="VFV87_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102595.1"
                     /translation="MVQLPIERAWQHLARLEDWPSWARHIRQIKVEPPGNLAAISKGS
                     LHLRNGIKPEFKVTEFNPYRNWKWVGGFLWATVHYDHFFEELNPTETKLTWVVEA"
     gene            complement(453..1892)
                     /locus_tag="VFV87_02215"
     CDS             complement(453..1892)
                     /locus_tag="VFV87_02215"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5102596.1"
                     /translation="MPTTAELMDALPESAGEITGMPQGIAPERLADWALRPVPVGAFR
                     RLRVLGTMQAEVTAAYLFLWLRGWFKDEAEKEKLRAETQWTTALRILDTMSYLRGATI
                     KIGQMMATFPDIMPGPFVETFEQLHFDAPPMHWSLLKEMVHNELGDEPDDRFATFNKR
                     AFAAASMGQVHRARLKSGEDVAIKVQYPGIARTIRDDFRNLFFFLLPARFSRNWKKLK
                     AQFEDLRLRLERETDYEQEADIQERVRKLFRDEDRIVVPRVYREHSTGRILAMDYLPG
                     QTLDQFVAANPSQKERNHLARLILRAWYRMLYAGRLFYADIHPGNFLLLDDGRLGLID
                     FGFMLELDNTLWQLFRNIDRPQTTGLREERRAALKEWDIVGDEPADQDLLRLSEELAD
                     WFWKCRYGRGEFDFGDESDFRHGVDLMVELGRARYSSGRPCALVIARQTFGIRSILYR
                     LKAKIDVKLLAEADIAMTGWDRSEYAQTT"
     gene            complement(1896..2528)
                     /locus_tag="VFV87_02220"
     CDS             complement(1896..2528)
                     /locus_tag="VFV87_02220"
                     /inference="COORDINATES: protein motif:HMM:NF036524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5102597.1"
                     /translation="MRVTAETRLATRKRILEASQQLIAAQGFEAATTRDIARAAGIGI
                     GTLFNYFPTKEAVAHTLIGEAYALAAQRFTVERARDHAERLSLEEELFAHIAAVLRKL
                     KPFRKYLTAVLDSHLSPLADRNGDAASFQAAHLETVGQIAARHGASESFTAVALQLYW
                     TLFVGVLAFWSNDASPRQEDTLALLDQSLAMFAGWLTSQANPNPSKHAGG"
     gene            complement(2829..>3418)
                     /locus_tag="VFV87_02225"
     CDS             complement(2829..>3418)
                     /locus_tag="VFV87_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102598.1"
                     /translation="AFCNRCPKWARSQCLAACQGCSGTANHLCGSCGNYVCADLASDT
                     NNCGECGYACPPPGLREYGVCIDGYCEYACAEGAVRCNGTCTYLSLDPDNCGACGNVC
                     GGPNPSCYDGVCGHCTPNCPEGWCGGDGCGGECACPSGTYCEQNGWCYDYCAPGLSWC
                     DPGCVDLSSDPFNCGACYRQCAPSEVCAGGICQGF"
BASE COUNT          625 a         1038 c         1074 g          681 t
ORIGIN      
        1 cgcttccacc acccaagtga gcttcgtctc tgtcggattt agctcctcga agaaatggtc
       61 gtaatgaacc gtcgcccaca aaaagccccc cacccatttc caattgcgat acgggttgaa
      121 ttcagtcacc ttgaactcgg gcttgatgcc attccttaga tggagcgagc ccttggagat
      181 ggctgcgagg ttccctggcg gctcaacctt gatctgccgg atgtgccgcg cccaactcgg
      241 ccaatcttcc agacgagcca gatgttgcca ggcccgctcg attggaagtt ggaccatgaa
      301 ctctcgtcgc aacaattcga tcatggttgc tcttcccgcg gggcctctgg tcgctcgtgt
      361 ttgcattggg aatcgatggc tagttcttga cggtcgctta cttgacggtt cgggcctcac
      421 tggccgcggt cgttttggat ttgtcgagtg ggtcaggtgg tctgggcata ctcgctccga
      481 tcccatccgg tcatggcgat gtccgcttcg gccaggagct ttacgtcgat cttggccttc
      541 aggcgataaa ggatggagcg gatgccgaac gtctgccgag caatgaccag cgcgcacggg
      601 cggccgctgg aatagcgagc acgcccgagc tccaccatca gatcgacgcc gtggcggaag
      661 tcggattcat cgccgaagtc gaactcacct cggccgtagc gacacttcca gaaccaatcg
      721 gccagttcct cgctcaagcg caggagatcc tggtcggccg gttcgtcgcc cacgatgtcc
      781 cattccttca gtgctgccct gcgctcttcg cggaggcccg tggtctgcgg ccggtcgata
      841 ttgcgaaata attgccaaag cgtgttgtcg agctccagca tgaagccgaa atcgatgagg
      901 cccaatcggc cgtcgtccag cagcagaaag ttgccggggt ggatgtcggc atagaatagg
      961 cggcccgcgt aaagcatgcg ataccatgcc cgcagaatca ggcgggcgag gtgattgcgc
     1021 tctttctgcg aggggtttgc cgccacgaac tgatcgagtg tctgcccggg caggtagtcc
     1081 atcgccagga tccgaccggt ggaatgctcg cgataaacgc gcggcacgac gatcctgtct
     1141 tcgtcgcgaa agagctttcg cacgcgctcc tgtatgtcgg cctcttgttc gtagtcagtt
     1201 tcccgctcca ggcggagacg cagatcctcg aactgcgcct tgagtttctt ccaattgcgg
     1261 ctgaagcgag cagggagcaa aaagaagaac aaattgcgaa aatcgtcgcg gatcgtgcgg
     1321 gcgatgccgg gatactggac ctttatagcg acgtcttcgc ccgatttgag gcgcgcccga
     1381 tgcacctggc ccatcgaggc ggcggcaaac gcccgcttgt tgaacgttgc gaagcggtcg
     1441 tcgggctcgt cccccaattc attatgcacc atttccttga gcagcgacca gtgcatcggt
     1501 ggggcgtcga aatgcagttg ctcgaaagtt tccacgaacg gtccgggcat gatgtcggga
     1561 aaggtcgcca tcatctggcc gatcttgatc gtcgccccgc gaagatagct catcgtgtcg
     1621 aggatgcgca gggcggtcgt ccactgcgtt tcggcgcgca acttctcctt ctcggcttca
     1681 tccttgaacc agccgcggag ccagagaaac aggtacgccg ctgtgacttc ggcttgcatc
     1741 gtgcccagga ctcggagccg ccgaaaggcg ccgacgggca cggggcgcag ggcccaatcc
     1801 gcgagccgtt cgggcgcgat gccctgtggc atgccggtga tttcacctgc cgattcgggc
     1861 agggcgtcca tcaattcagc cgtcgtgggc atggtttacc ctcctgcgtg tttcgatggg
     1921 ttgggattcg cctgactcgt cagccaaccg gcaaacattg ccagggactg gtcgagcaag
     1981 gccagcgtgt cttcctgccg cggtgaggca tcgttggacc agaacgcgag gacgccaaca
     2041 aagagcgtcc aatagagttg caaggcgacg gccgtgaatg actcgctggc gccgtgacga
     2101 gctgcgatct ggccgacggt ctccagatga gcagcctgaa acgacgccgc atcgccgttc
     2161 cggtccgcca gtggagaaag gtgcgagtcc aacacggccg tcaaatactt gcgaaacggc
     2221 ttgagcttgc ggagcaccgc ggcgatgtgg gcgaacagct cctcttcgag cgaaaggcgt
     2281 tcggcatgat cgcgagcccg ctccaccgtg aatctctgcg ccgcgagtgc ataggcttcg
     2341 ccaattagcg tgtgggcgac ggcctccttg gtcggaaagt aattgaacag cgtgccaatc
     2401 ccgatccccg cggcgcgggc aatgtcgcgg gtggtggcgg cttcgaagcc ctgcgcggcg
     2461 atcaactgtt gcgaggcttc gaggatccgc ttgcgggtag ccaatctggt ttccgctgtg
     2521 acacgcatga cacaggcctg gtcaacacgt cgtccaatta aatgagcacg ctcacattgt
     2581 atcggccatc gaccggccgt caagtcataa aatgagcgtg ctcgcataat tttcgtcctg
     2641 gatcaacttc agggtttgtc gagccaccgg acgtgcgcct gggcaccgcg ccttcgggcg
     2701 gttcgggccg gtgaggagct tgcttgacgg tcagggcctc accggccgct tttgttgcgc
     2761 gctggtcggc cccttcaggg ccgacgagac ggcctggaca tgccgtccca cgcgccgctt
     2821 gttttgattt agaacccttg gcagattcct ccggcacaaa cctcagacgg tgcgcattgg
     2881 cgatagcagg cgccgcagtt gaatgggtcc gaactgagat caacgcaacc ggggtcacac
     2941 cagctgaggc cgggcgcgca gtagtcgtag caccagccgt tttgttcgca gtacgtgccg
     3001 ctggggcaag cgcactcccc gccacaacca tcgcctccgc accatccctc cgggcaattc
     3061 ggcgtgcagt ggccgcagac tccgtcgtag caggacggat tgggtccgcc gcagacgttg
     3121 ccgcaggcgc cgcagttgtc ggggtcgagg ctcaagtacg tgcaggtccc gttgcagcgg
     3181 acggcgccct ccgcgcaggc gtactcgcag tagccgtcga tgcagacgcc gtactcgcgg
     3241 aggccgggtg ggggacacgc gtagccgcac tctccgcagt tgttggtgtc gctggcgaga
     3301 tcggcgcaga cgtagttgcc gcacgacccg cagagatggt tggcggtgcc gctgcacccc
     3361 tggcaagcgg cgaggcattg cgaccgggcc cacttcgggc accggttgca gaaggctt
//
LOCUS       DASUGB010000117         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_569781, whole genome shotgun sequence.
ACCESSION   DASUGB010000117 DASUGB010000000
VERSION     DASUGB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569781"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1441)
                     /locus_tag="VFV87_02230"
     CDS             complement(44..1441)
                     /locus_tag="VFV87_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5102599.1"
                     /translation="MSTTEEFEDLECIGRGGFGEVLRCRRKSDGKVFARKRILAFAKQ
                     RTASDDVRRFVREVRLLSRLAHPNIIPIIAANTSTQPFWYVTPLYSDSLMTYLPEIAC
                     RHEQIHAVMAKVMAGVNFAHKNGILHRDLSLGNVLMNSLDDVVVCDFGLARSLEDVDS
                     TRTVSLQFFGTRYFMPPEALDMGSKYADERGDIYSIGRLIVALYLGVLKHNFQGVIGL
                     PETVRRCVNSLASDYDSRLESMDEAEQAWRRVPVTLDNWESFSLDLLRTAVSYADGGN
                     REAAIRDAIRHIESQKLFLTTAGEGHAVIPAALAGHYAAFSRAIFSFVCVAPLEMLLY
                     FQRARPKLFVQIIRHAARFLAGAIDELPAIFRRDMLARAHELFSQIDSVEVRLELMII
                     ALTVLVAQQGKRATAETVVNSMFTESFESSEMAVLCKGLRSWWKKRGGKKTEMPPVRA
                     EEFAEPFQEFFASCR"
     gene            complement(1541..3664)
                     /locus_tag="VFV87_02235"
     CDS             complement(1541..3664)
                     /locus_tag="VFV87_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102600.1"
                     /translation="MAPRASAETLIDLSGYHPGCEVEVAGWNGHLKIQWPITEVETGE
                     LTLDTSGNAPLIEKLVVRQNSVEAVILKDVDPVWFLTVGSREMPPGKPPEQKWEVFFD
                     NPHRRPHEVFASKLAIAKAKVAGSGSRASVTIDGLAIGPFAGSLEFSFYAGSPLVRVD
                     AVVATEKDGVAMFYDAGIVADEPSWKDVGWADTEGHYHFVLTRDLKQPASTVKVRGRV
                     IAVDSVPHGVVACFPPPHQFQFPRDYSTNLGFVWHGKGYQGLENKVGFGIRQNKDGGG
                     NFVPWFNAPPGTQQRLGMFLLVSSGNAQESLDDTFAYTRGDRFAELPGYKTLTSHWHM
                     AIAVAAMEEAKRGIQRTEQPEYARIFREMGVNMVHLGEFHGDGHPKDPGPLRLPEMQA
                     MFEECRRWSDDELLLIPGEEINDFLGLKTQGKHPGHWMSLFPRPVYWTQTRAADQPFM
                     EEHPQYGKVYHVGSRGDMIRLIKEERALVWSAHPRIKASSWTPDIFREEDFFLAEYWL
                     GGAWKAMPGDLSRERLGERVLNLLDDMANWGHKKYVLGEVDVFKIDHTHELFAHMNVN
                     YVQMDRVPKYDEGWQPVLDTLSNGKFFVTTGEILIPKFTVGGKPSGDVLKRGDSATAS
                     PEPRSPNPELLADLQWTFPLAFAEVISGDGKQVYRERIELSDTKPFGSRTLKLTPDLR
                     GRHWVRLEVWDIAANGAFTQPVWIE"
     gene            complement(3936..>4385)
                     /locus_tag="VFV87_02240"
     CDS             complement(3936..>4385)
                     /locus_tag="VFV87_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102601.1"
                     /translation="DHSGRPFYIREFVEGTTLLDLGAKGMINLTSAIGDLIDVANVVE
                     RVHGLGLVHRNLSPENVLIARDGMPWLIGFGRVRRLQDLRLSNAEPPFGAIIDVDVRG
                     LQRLLKWICQAIHDPIPPGLEDAQQPDAFANAREFAEGITAFSQGPD"
BASE COUNT          834 a         1378 c         1353 g          820 t
ORIGIN      
        1 cctccacttg gataacgcct gccgcaattg actcccgacc ccttcaccgg caactcgcaa
       61 agaactcctg gaacggctcg gcgaattctt cggcgcggac gggtggcatc tcggtcttct
      121 tgccgccgcg ttttttccac cacgagcgga gacccttgca gagcacggcc atctcgctcg
      181 attcgaacga ctcggtgaac atcgagttga ccaccgtctc ggccgtggcc cgcttcccct
      241 gctgggccac caggaccgtc agggcgatga tcatcaactc cagccggact tcgacgctgt
      301 cgatttgcga gaacagctcg tgggcgcggg ccagcatgtc gcggcggaag atggcgggca
      361 attcgtcgat cgccccggcc aggaaccggg ccgcgtggcg gatgatctgc acgaagagct
      421 tcggccgggc ccgctgaaaa tagagcaaca tctccagcgg cgcgacgcag acaaagctga
      481 atatcgcccg cgaaaacgcg gcgtaatgcc ccgccagtgc cgccggaatg accgcgtgcc
      541 cctcgccggc ggtcgtcagg aacagcttct gcgattcgat gtgccggatt gcgtcgcgga
      601 tcgccgcttc gcggttgccc ccgtcggcat acgagacggc tgtccgcagg agatcgagcg
      661 agaacgattc ccagttgtcc agcgtcaccg gcacgcgccg ccaggcctgc tcggcttcgt
      721 ccatgctctc cagccggctg tcgtaatcgg aggcgagcga gttgacgcac cgccgcaccg
      781 tctcgggcag gccgatgacg ccctgaaaat tgtgcttgag cacgcccagg tagagggcca
      841 cgatcaggcg gccgatcgaa tagatgtctc cccgttcgtc ggcatatttg cttcccatgt
      901 cgagggcttc cggcggcatg aagtaccgcg tgccgaaaaa ctgcagcgag acggtccgcg
      961 tcgaatcgac gtcctccagc gaccgcgcca acccaaaatc gcagacgacc acgtcgtcga
     1021 gcgagttcat cagcacgttg cccagcgaca gatcgcgatg cagaatgccg ttcttgtggg
     1081 cgaagttgac ccccgccatc accttggcca tcaccgcgtg aatctgctcg tggcggcagg
     1141 caatctccgg cagataggtc atcagcgaat cggaatacag cggcgtcaca taccagaacg
     1201 gctgcgtgga cgtattggcc gcgatgatcg gaatgatgtt ggggtgcgcc aatcgcgaca
     1261 gcagccgcac ttcgcgcacg aaccgccgca cgtcgtcgct cgcggtccgc tgcttcgcga
     1321 aggccaggat ccgctttcgc gcgaagacct tgccgtccga cttccgccgg caccgcaaca
     1381 cctcgccaaa tccaccgcgg ccaatgcact ccaggtcctc gaactcttcc gttgtggaca
     1441 tgacggaaat tcgggcgtgg ttttaacgtg ggctcaatgc ggatgatgct ggaacggcat
     1501 tttaagggcc gacgcccaat accatccagg cgagtcgttc tcactcaatc cataccggct
     1561 gcgtgaaggc gccgttggcg gcgatgtccc agacttcgag gcggacccag tgccggccgc
     1621 gcagatcggg cgtgagcttg agcgtgcggc tgccgaaggg cttggtgtcg gatagctcga
     1681 tgcgctcgcg atagacctgc ttgccgtcgc cggagatgac ttcggcaaag gcgagcggga
     1741 aggtccactg gaggtcggcg aggagctcgg ggttcgggga tcggggttcg gggctcgcgg
     1801 tggcgctgtc gccgcgtttc agcacgtcgc cgctgggctt gccgccgacc gtgaacttcg
     1861 gaatcagaat ctcgcccgtc gtgacgaaga acttgccgtt agaaagcgtg tctagcactg
     1921 gctgccagcc ttcgtcgtac ttggggacgc ggtccatttg cacgtagttg acgttcatgt
     1981 gggcgaacaa ctcgtgcgtg tggtcgatct tgaacacatc gacttcgccc agcacgtatt
     2041 tcttgtggcc ccagttggcc atgtcgtcga ggaggttcag cacccgctcg cccagccgct
     2101 cgcgcgagag gtcgccaggc atcgccttcc acgcgccgcc cagccagtac tcggcgagga
     2161 agaagtcctc ctcgcggaag atgtcgggcg tccagctcga agccttgatt cgcggatgcg
     2221 ccgaccagac cagggcccgc tcttccttga tcagtcggat catgtcaccg cggctgccga
     2281 cgtgatagac cttgccgtac tgcggatgct cctccatgaa gggctggtcg gcggcgcggg
     2341 tttgcgtcca atagacgggt cgcgggaaga ggctcatcca gtggccggga tgcttgccct
     2401 gggttttcag gccgaggaag tcgttgatct cttcgcccgg aatgagcagc agttcgtcat
     2461 ccgaccagcg gcggcattcc tcgaacatcg cctgcatttc cggcagccgc agcggaccgg
     2521 gatccttcgg atgtccgtcg ccgtggaatt cgccgaggtg aaccatgttc acgcccatct
     2581 cgcggaagat gcgggcgtat tcgggctgct cggttcgctg gatgccgcgt tttgcttcct
     2641 ccatcgcggc gacggcgatc gccatgtgcc agtggctggt gagcgtcttg tagccgggaa
     2701 gctcggcgaa gcgatcgccg cgggtgtaag cgaacgtgtc gtccagcgat tcctgggcat
     2761 tgccggacga aacgagcagg aacattccga ggcgctgctg ggtgccaggc ggggcgttga
     2821 accaggggac gaagttgccg ccgccgtcct tgttctggcg aatgccgaag ccgaccttat
     2881 tctccagtcc ctgatatccc ttgccgtgcc aaacaaagcc gaggttggtg ctgtaatcgc
     2941 gcgggaattg aaactggtgc ggcggcggaa agcaggcaac tacgccgtgt gggacactat
     3001 cgactgcaat aactcttccg cggaccttga cggtagaggc gggttgcttc aggtcgcggg
     3061 tcagaacgaa atggtaatgg ccctcggtat cggcccagcc cacgtccttc caagagggct
     3121 cgtcggcgac gattcccgcg tcgtaaaaca tcgccacgcc gtccttctcg gtcgcaacga
     3181 ccgcatcgac gcggaccagc ggcgagccgg cgtagaacga gaactccagc gagcccgcaa
     3241 aaggtccaat cgcgaggccg tcgatcgtca cgctcgcccg cgagccgctg ccggcgacct
     3301 tggccttggc gatcgccagc ttcgacgcga acacctcatg cggccggcgg tgcgggttgt
     3361 cgaaaaacac ttcccatttc tgctcgggcg gcttgccggg gggcatttcg cggctgccga
     3421 cggtcagaaa ccagactgga tcgacatcct tcagaatcac cgcttcgacc gaattctgcc
     3481 gcaccacgag cttctcgatc agcggggcgt tgccgcttgt gtcaagcgtc agctcccccg
     3541 tttcgacttc cgtgatcggc cactgaatct tcaagtgacc gttccacccc gccacttcta
     3601 cctcgcaacc ggggtgatag cccgacaggt cgatcagcgt ttcggcggag gcacgtggcg
     3661 ccaaagcgaa agtaaggagc gcaaccaaga ttggcgcgcg gaaagcgacg ttaaatgaac
     3721 cggtatccat ggcaaaggct ttcctgtcac ggcgagatcg aaccaaacgc ggctgcggcc
     3781 tcattctacg tcatctgcac cgcggctgcc gtaccaaccg cgctcgaaac gccggcgtca
     3841 cgatgtggag gccgaaggag tttgcggcgg cggatgaagc atcagctcgg ctgcaactaa
     3901 acatcgaact gctgaccaac atacggtcgt acgcatcaat cgggaccttg actgaaggcg
     3961 gtaatgccct cagcgaattc ccttgcattg gcgaatgcgt cgggttgctg ggcatcctca
     4021 agaccagggg gaatcgggtc gtgaatcgct tggcatatcc acttgagcag gcgttggaga
     4081 ccgcgcacat ccacgtcgat gatcgctccg aaaggcggct cggcattaga aagtcgcaaa
     4141 tcttgaagcc gcctgactct gccgaagcca attagccaag gcatgccatc tctggcaatc
     4201 aggacattct ccggcgacaa gtttctgtga accaacccta gcccatgcac ccgttccaca
     4261 acgttagcga cgtcgatgag atccccgatg gctgacgtga ggttgatcat gcccttcgcg
     4321 ccaaggtcca gcaaggttgt accctccaca aactcgcgaa tgtaaaaggg ccgaccggag
     4381 tgatc
//
LOCUS       DASUGB010000118         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1820927, whole genome shotgun sequence.
ACCESSION   DASUGB010000118 DASUGB010000000
VERSION     DASUGB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820927"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VFV87_02245"
     CDS             <1..1568
                     /locus_tag="VFV87_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102602.1"
                     /translation="IEIAQFAGAAPRGLKLQYEQTLQISIRNGWGEPLTFASLRASDG
                     SYRRYVSVSNNSSLNAKAHGHLVVVNFGAEIIGIDALRGERTATDSLLWRQDSADLDT
                     ISQRTIYPSQRSVSNPFTGSRYVSYDPSGRMNFDLGPMQTSGLCYQKGRQLICVDLLT
                     GESIWERSQVPAQAEIFGDGEFIFVADASGDEVQVISAIDGALAGRRKIDRSDRRWTT
                     RGRNVLAWEQTGTTLKLRLYDAWQQDAQGQHPDLWSRQVPQGTKGFVIDGEELALLEP
                     SGQFTVVSLDTGELRFAVPLDPEPALDSILVQRTSDQYVLMTNQNNPTPPPGLLPSPV
                     PNSAQVRLHGRAYAFARSTGKLQWQVPAFVAAHCLLADQPTETPLLLFVRNQRQVGEG
                     NNNLTNCHVLCLDKRDGRVVFDNVVGQQAMSCDAVADPVKRTVTMTIATQPKPTVLTF
                     TATDKPSAPQPPAQTGEAASDSVGQLPGTVDRSVGAAIERLNRGADPSQLAPLPPPVR
                     LRAPNAQPSAPPR"
     gene            complement(1592..1726)
                     /locus_tag="VFV87_02250"
     CDS             complement(1592..1726)
                     /locus_tag="VFV87_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102603.1"
                     /translation="MPRLLLLLLIVACLANAGCAAIGTAAGLYALSQTKFVQEQLDDL
                     "
     gene            complement(1806..2615)
                     /locus_tag="VFV87_02255"
     CDS             complement(1806..2615)
                     /locus_tag="VFV87_02255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102604.1"
                     /translation="MGLASDLKILYHLALKPVRGKDHAARMESFYSGQAEAYDDFRKR
                     LLKGRQELWNLIQPPEGGTWIDMGGGTGGNLEYFQDVGWDKAADAQRSAAAGPPPSAA
                     DGGPTLQKLRKVYVLDLSHSLLEVAKKRIAAKGWTNVETVEADATTFQPPDGPVDVVT
                     FSYSLTMIPDWFAAIENALAMLKPGGQIGVVDFYVSRKFPQDGLKQHGWSTRSFWPTW
                     FAMDNVFPSPDHVPFLHRHFEVLAFEEHRAKVPYVPFSRVPYYLFVGRKRS"
     gene            complement(2707..>3159)
                     /locus_tag="VFV87_02260"
     CDS             complement(2707..>3159)
                     /locus_tag="VFV87_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEX5102605.1"
                     /translation="YFWRVYMKGEYSRGCCPEYLKAENFEVLKSGLVERVSTHSNSVQ
                     GFLDTHEGPISRYVLLDHMDWLSDKFFPLLELEWNAIVRRAAPETRIIWRSGGLKTDF
                     LDRVNVSSRGQICPLPELLTHHADLAAELHEKDRVHTYGSFYIADLAA"
BASE COUNT          596 a         1021 c         1005 g          537 t
ORIGIN      
        1 cgatcgagat cgcccagttt gccggtgcgg ctccgcgcgg cctcaaactg cagtacgagc
       61 agaccctgca gatcagcatc cgcaacggat ggggcgagcc gttgactttc gcctcgttgc
      121 gggccagcga cggcagctac cggcgctacg tttcggtgtc gaacaacagc tcgctgaacg
      181 ccaaggccca cggccacttg gtcgtcgtaa acttcggcgc ggagatcatc ggcattgacg
      241 ccctgcgggg cgagcggacc gcgaccgatt cactcctctg gcggcaggat tctgccgacc
      301 tcgacacaat ctcgcagcgg acgatctacc cttcgcagcg ctccgtgagc aatcctttca
      361 cgggctcgcg atacgtcagt tacgatccta gcgggcgaat gaacttcgat ctgggcccga
      421 tgcaaacgtc ggggctgtgc tatcagaaag gccgtcagct gatctgcgtc gatctgctca
      481 cgggcgaatc gatctgggag cgcagccagg tgccggcgca ggccgagatt tttggcgacg
      541 gcgagttcat cttcgtggca gacgccagtg gcgacgaagt gcaggtcatc agcgccatcg
      601 acggcgcctt ggcgggccgg cgcaaaatcg accgctccga ccgccgctgg acgacgcgcg
      661 gccgcaacgt cctggcctgg gagcagaccg gcacgacgct caagctccgc ctctacgacg
      721 cctggcagca ggacgcccag gggcagcacc ccgatctctg gtcgcgccaa gtcccgcagg
      781 gcaccaaggg attcgtcatc gacggcgagg aactggcact gctggagccg agcgggcaat
      841 tcaccgtcgt ctcgctcgat acgggcgaat tgcgattcgc cgtgccgctc gatcccgagc
      901 cggcgctcga ttcgatcctg gtgcagcgga ccagcgatca atacgttttg atgacgaacc
      961 agaacaatcc aacgcccccg ccgggtctgc tgcccagccc ggttccgaat tccgcgcagg
     1021 tccggctgca cgggcgtgcc tacgccttcg cccgctcgac cggcaaattg cagtggcagg
     1081 tgccggcgtt cgtcgccgcg cactgcctgc tggccgacca gccgacggaa acgccattgc
     1141 tcttgtttgt tcgcaatcaa agacaggtcg gagaaggaaa caacaacctg accaactgcc
     1201 acgtgctctg cctggataag cgcgacggac gcgtcgtctt cgacaacgtc gtgggtcagc
     1261 aggcgatgag ttgcgacgcc gtggccgatc cggtcaagcg gaccgtgacg atgacaatcg
     1321 ccacgcaacc gaagcccacg gtcctgacgt tcaccgcgac cgacaaaccg tccgccccgc
     1381 agccaccggc gcaaacgggc gaagcggcca gcgacagcgt gggtcagctc ccgggaaccg
     1441 tcgatcgctc ggtcggggca gcaatcgagc ggctgaaccg cggcgcggat ccgtcgcagc
     1501 ttgcgccttt gccgccgccg gtccggttgc gggcgccgaa tgcgcagccc tctgcaccgc
     1561 cacgctgatt gcgagtctgc gaggcacgat tttacagatc gtccaactgc tcctggacga
     1621 acttcgtctg ggaaagcgca tacagcccgg ctgccgtgcc gatcgcggcg cagcccgcat
     1681 tggccaggca cgcgacgatc aacaggagca ggagaagacg cggcataggc gccctgcgcg
     1741 acaaggacca gcgagaagag aagcacgccc agacgggcgg gagaatagcg acgcggctgg
     1801 gcgtgtcaag atcgttttcg gccgacgaac aggtaatagg gcacgcggga aaaaggaacg
     1861 taaggcacct tggcccggtg ctcctcgaat gccagcacct cgaaatgccg gtgcaggaag
     1921 gggacgtggt cgggcgacgg gaagacgttg tccatcgcga accacgtcgg ccagaagctc
     1981 cgcgtgctcc agccgtgctg tttcaatccg tcctgcggga acttgcgcga gacatagaaa
     2041 tcgacgacgc cgatctgtcc gccgggcttg agcatcgcga gagcgttctc aatcgccgcg
     2101 aaccagtcgg gaatcatcgt cagcgagtag gaaaacgtga cgacatcgac cggcccgtcc
     2161 ggcggctgga aggtcgtcgc gtcggcttcc acggtttcga cgttcgtcca gcccttggcg
     2221 gcgatccgct tcttggcgac ctccaagagc gagtgcgaga ggtcgaggac atagaccttg
     2281 cgaagttttt gtagggtggg cccaccgtcg gcggcgcttg gtggtgggcc tgcggccgcc
     2341 gagcgctgcg cgtcggcggc cttgtcccac cctacatcct ggaaatactc gaggttcccg
     2401 ccggtcccgc cccccatgtc gatccaggtt ccgccttccg gcggctggat caggttccac
     2461 agctcttgcc gccctttgag cagccgcttg cggaaatcgt catacgcctc cgcctgcccg
     2521 gaataaaaac tctccatccg cgcggcatga tccttcccgc gcactggctt gagtgcgagg
     2581 tggtagagga tcttgagatc ggaggcgagg cccactgcag agtgggagag ggggagagtg
     2641 ggagagaggg agaatatcgg tctccccctc tccccctctc ctcgtctccc tctcctgaat
     2701 tgagacttag gcagctagat cagcaatgta aaaactaccg tacgtatgca cgcggtcctt
     2761 ctcatgcagc tcggcggcga ggtcggcgtg gtgcgtgagc agctcgggga gcgggcaaat
     2821 ctggccgcgg gagctcacgt tcacgcggtc gaggaaatcg gtcttcaggc cgccgctccg
     2881 ccagatgatc cgcgtttctg gcgcggcgcg gcggacgatc gcgttccact ccagctccag
     2941 gagcgggaag aacttgtcgc tcagccagtc catgtgatcg agcagcacgt accgcgaaat
     3001 cggcccttcg tgcgtgtcga ggaatccctg aacggagttg gagtgcgtgc tgacgcgctc
     3061 gaccagtccg ctcttgagca cttcaaaatt ctcggccttc agatattccg ggcagcagcc
     3121 gcgactgtac tcgcccttca tatacacgcg ccaaaagta
//
LOCUS       DASUGB010000119         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1960264, whole genome shotgun sequence.
ACCESSION   DASUGB010000119 DASUGB010000000
VERSION     DASUGB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960264"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VFV87_02265"
     CDS             complement(<1..1353)
                     /locus_tag="VFV87_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102606.1"
                     /translation="MPAFPLPAAVARRTFLSQSGMSLGAMALSSLLAGSVRGATSEIR
                     SRGAIRELHHPARIKRVIWLYMAGGPSHLETFDYKPELARRSGQPMPESFTKGQPIAQ
                     LQGRSQLKCLGPLFDFSRHGKSGQEVSSALPHIAGIADDLCILRGLVTEAINHDPAHT
                     FMNTGTTISGRPSMGSWLTYGLGSEAEDLPGFVVLTSFAGRSPQPISNRMWHNGFLPG
                     KFQGIKLNSTGSAVEYLDSPAGVSQRQQREVIDAVRALNQAEDRRAADPDIETRLAQY
                     EMAFRMQVSVPRLMDFSDESQATLDLYGARPGDGSFASNCILARRMAERGVRFIQLYH
                     RDWDHHNDLKHFVGVCAGACDRPSAALVQDLKQRGMLDETLVVWGGEFGRTPMAQDGK
                     GEQLGRDHHMKGFSMWLAGGGIKPGITHGATDELGYNSVDNIVHVHDLHATILHLLGI
                     D"
     gene            1719..>3039
                     /locus_tag="VFV87_02270"
     CDS             1719..>3039
                     /locus_tag="VFV87_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102607.1"
                     /translation="MPRLLSSRALLWLTAAAAWAVARPAGAEEAPPVFSAEQLQFFER
                     EVKPILRANCIACHGGEKKVQGGFHLTSRASVLKGSENGPVVSLENPAESSLLEAINY
                     QSLEMPPKGKLPQAQIETLTRWVKLGLPWPDGEAGNLVQHGPPVVDEAARNFWSFRAV
                     VRPPPPAVKDQAWVKSPIDAFILARLEEKGLRPAPPAEKAALLRRVYYAVIGLPPSPA
                     QVAAFLADESPTAYEKVVEELLASRHYGEHWGRHWLDLVRYAESNSFERDNPKPFVWR
                     YRDYVIRSLNADKPYDQFLREQLAGDELESVTPDGIIATGYYRLGLWDDEPADPLLAY
                     YDGLDDIVATTGQTFLGLTVNCCRCHDHKLDPLPQRDYYKFLAFFHGIKHYGVRSDES
                     VAEASLVSIADVQEQDKHRQLIAQYEAKIADNGRQQQEIEDQVAGKLP"
BASE COUNT          533 a          989 c          989 g          528 t
ORIGIN      
        1 gtcgatgccc aacaggtgca ggatcgtcgc gtgcaggtcg tggacgtgga cgatgttgtc
       61 gaccgagtta taccccagct cgtccgtcgc tccgtgcgtg atgcccggct tgatgccgcc
      121 gccggccagc cacatcgaga agcctttcat gtggtgatcg cggccgagct gttccccttt
      181 gccgtcctgg gccatcggcg tgcggccgaa ttcgccgccc cagacgacca gcgtctcgtc
      241 gagcatgccc cgctgcttta gatcctgcac caaagcggcc gacgggcggt cgcacgcgcc
      301 ggcgcagacg ccgacaaagt gcttgaggtc gttgtggtgg tcccagtcgc ggtggtagag
      361 ctggatgaac cgcactcccc gctcggccat tcgccgggcc aggatgcagt tgctggcgaa
      421 cgagccgtcg ccgggccggg cgccgtacag atcgagcgtg gcctgcgatt cgtccgagaa
      481 atccatcaac cgcgggacgc tcacctgcat ccggaacgcc atttcgtact gggccaggcg
      541 agtttcgatg tcgggatcgg ctgcgcggcg atcttcggcc tggttgagcg cccgaaccgc
      601 gtcgatcacc tcgcgctgct ggcgctggct gacccccgcg ggcgagtcga gatactccac
      661 cgctgagccg gtcgagttga gcttgatccc ctgaaacttg cccggcaaaa agccgttgtg
      721 ccacatccgg ttgctgatcg gctgcgggct gcggccggca aagctggtga ggacgacaaa
      781 gccgggcagg tcttcggcct cgctccccag gccgtaggtg agccaactgc ccatgctcgg
      841 ccggccactg atcgtcgtgc cggtgttcat gaacgtgtgg gccgggtcgt ggttgatcgc
      901 ctcggtcacc aggccgcgga ggatgcagag gtcgtcggcg attcccgcaa tgtgcggcag
      961 ggcggagctg acctcctggc ccgatttgcc gtgccgggaa aaatcgaaca gcggccccag
     1021 gcacttcagt tgcgagcggc cctgcaactg ggcgatcggt tgccccttgg tgaacgattc
     1081 gggcatcggc tggccggacc gcctggccag ctcgggcttg taatcgaacg tctccaggtg
     1141 cgatggtccg cccgccatat acagccagat cacccgcttg atcctggctg ggtggtgcag
     1201 ctcgcgaatc gcgccgcgcg agcgaatctc gcttgttgca ccgcgcacgc tgccggccag
     1261 cagcgacgac agggccatcg cccccaggct cattcccgat tgactcaaaa aagtgcggcg
     1321 ggcaacggca gcgggcaatg gaaaggcggg catcactgtc tccggcaggc ggctgaaaag
     1381 ggcagaacat cattctcgcc cggcagtgca ttgacagcaa ccgaaagcgg ccgctggttt
     1441 tggccggttc gtttcaacgc gaagacgcag tggaaacgca aagtccccat gacggcagat
     1501 gcgaatcctt ctttccgctc tgcgtctgag ctttccctgg cgccttggcg ataaggattg
     1561 atcgctaccg ctgattcaat gcaaccgctc ggaacttgca ggttttggcc tcgttcacta
     1621 gactaaattt cacacgccga aagcggcaca gcccgcaatt gccgccggcg tgccccgccc
     1681 acgccgcccc tcatttccgc gctgcgagac tttgttcgat gcctcgcctg ctgtcatctc
     1741 gcgcgttgct ttggctgacg gcggcggccg catgggccgt agcccggcca gccggcgcgg
     1801 aagaggcacc gcccgtgttt tcggccgagc agctccagtt ctttgagcgg gaagtcaagc
     1861 cgatcctgcg ggccaactgc attgcctgcc acggcggtga aaaaaaggtg caagggggct
     1921 tccatctgac cagccgggcc agcgtcctca agggaagcga aaacggcccg gtggtttcgc
     1981 tggaaaatcc ggcggaaagc tcgctcttgg aggcgatcaa ctaccagtcg ctggagatgc
     2041 ctcccaaggg gaagctgccg caggcacaaa tcgagacgct cacccgctgg gtcaagctgg
     2101 gactgccgtg gccggacggt gaagcgggca acctggtgca gcacgggccg ccggtggtgg
     2161 acgaggcggc ccgcaatttc tggtcgttca gggcggtggt gcgcccgccg ccgcccgcgg
     2221 tgaaggacca ggcctgggtg aagtcgccga tcgacgcgtt tatcctggcc aggctcgaag
     2281 aaaaagggct gcggcccgcg ccgcctgctg agaaggcagc cctgctgcgg cgggtctatt
     2341 atgccgtgat cgggctcccc ccatcgcccg cacaggttgc ggcgtttttg gccgacgagt
     2401 cgcccaccgc ctacgagaag gtcgtcgagg agctgctcgc ctcgcgccat tatggcgagc
     2461 attgggggcg gcactggctc gacctggtgc gctacgccga atcgaacagc ttcgagcgcg
     2521 acaatcccaa gcccttcgtc tggcgatatc gcgactacgt catccgttcg cttaacgccg
     2581 acaagccgta cgaccagttc ctccgcgagc agctcgcggg ggacgagctc gaaagcgtga
     2641 cgcccgacgg gatcatcgcc accggctact accgcctggg cctgtgggac gacgagccgg
     2701 ccgatccgct cctggcatat tacgacgggc tggacgacat cgtggcgacg acgggacaga
     2761 cgttcctcgg gctgacggtg aactgctgcc gctgccacga ccacaagctc gacccgctgc
     2821 cgcagcgcga ctattacaag ttcctggcgt tctttcacgg catcaaacac tacggcgtgc
     2881 ggagcgatga atccgtcgcc gaggcctcgc tggtatcgat cgccgacgtc caggagcagg
     2941 acaagcatcg ccagctgatt gcccagtacg aggcaaaaat cgccgacaac ggccgccagc
     3001 agcaggagat cgaggaccag gtcgccggca agctacccg
//
LOCUS       DASUGB010000120         4805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1264463, whole genome shotgun sequence.
ACCESSION   DASUGB010000120 DASUGB010000000
VERSION     DASUGB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264463"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFV87_02275"
     CDS             <1..223
                     /locus_tag="VFV87_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102608.1"
                     /translation="FAWWLAWLAILATGVVGNYLDGSAVTLVDYSMAMRCDILFCLSI
                     IVAGVLAIYLMVRISQRQWQRYLAVAHDE"
     gene            complement(252..983)
                     /locus_tag="VFV87_02280"
     CDS             complement(252..983)
                     /locus_tag="VFV87_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102609.1"
                     /translation="MTDATEPITIDFRIPGTWTHPRELVQALPPGCRMTGEALILEDG
                     TEVEFGAMEADGQFAEIFCSSCRRPPTDEELERVERYTVNVILSGSGGSLEQAHAMMR
                     AAAAMVKAGGAGVFIDNSGLAHGGEIWLEMTEEGSSDAISFAYVAIIGNASEVWTMGM
                     HALGLREVVMKKADCDAFDITEVIRYLARGDKPVANGHVLADLDGPQFQCIVQDSPSE
                     RLRGAMRNPFGRLKLVSVRDIGEIN"
     gene            1212..1667
                     /locus_tag="VFV87_02285"
     CDS             1212..1667
                     /locus_tag="VFV87_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102610.1"
                     /translation="MGRMELQPETLEQIQQLIVANFAGRDELYAAADTLDDGARRDVC
                     RRLAEFLAGHAVELQQILKANGQAPAGPVAVDPIATAFFDSAQCSRGQVGVLAAAAER
                     ERDLKEGYQHALEKVSHPSAENVLRRHQKEVEFVEQVLRGMGAAEIEGQ"
     gene            complement(1687..2229)
                     /locus_tag="VFV87_02290"
     CDS             complement(1687..2229)
                     /locus_tag="VFV87_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102611.1"
                     /translation="MIIVWGTINAGKVDQAPGGMFHVMTRFGHVYYLPLSPTGSYLVL
                     EQTAEGFRGMPIPMSFKSILTAWVRAGSIVAIIASLVLTMIFLADVKAAPFGWIVPVL
                     VCIGAIVALFLSYRLKFFTEASYERAKELAGLMGMNEVGLLMIEVAYGRLTAEQAEQM
                     VASQIASSPDGSPIAAQLAD"
     gene            2254..2508
                     /locus_tag="VFV87_02295"
     CDS             2254..2508
                     /locus_tag="VFV87_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102612.1"
                     /translation="MQTGPAKIAIRRAASRKFRQIIAKNHNTSQPSTGDGLSRYWANG
                     SSEPLSDDPFAHAPRAPEIHACLTNGPSQRAPNDQNIHAL"
     gene            2498..3241
                     /locus_tag="VFV87_02300"
     CDS             2498..3241
                     /locus_tag="VFV87_02300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102613.1"
                     /translation="MLFNNPISGEKADRMVRLIELQPESHVLDVGCGTGELLLRIVGQ
                     HRARGVGVDVSPQCITAARKAAAERSLAERCEFHEFNVRDVELCRNTFDLGICVGATH
                     AFGPGEAAYPNSISWLRQMVRPGGYVLIGEGYWKRSPDAEYLQRLGDPVGIYRDHAQN
                     VSFAEQCGLEALYAVTSSDDEWDEFEWSHYQQSRRAAEASPDDQQLVARLARSIRWRD
                     GYLRWGRSTMGFGLYLFRVPRESVSAEVK"
     gene            complement(3251..4654)
                     /locus_tag="VFV87_02305"
     CDS             complement(3251..4654)
                     /locus_tag="VFV87_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102614.1"
                     /translation="MLTVWGSKHRFCDRLRRRDFLHIGLAGGALSLPSLLRLRAAAAA
                     EGNSQRQRPANKAVIMVCLNGGPSHLDMYDLKPQSPAEIRGEFRPIRTNVPGFEISEL
                     MPLQATIADKLAIVRSMQWPRDDGHHLHLVFSGFPKTAERPSFGSIVSRVRSERGQRD
                     ALPPYVSMAQFHTHPVLVGHEEPSYVGAAHQPFVPYQPGALINGQINYSGPGMGEVQN
                     LSPTAGLTRDRLTSRTTLLGTLDQLRSEIDARGEMAGQDAFTQQALDMISSARVREAF
                     DLSREPAAMHALYGGDIEYQDDPDPRRCWEGSKFLMARRLVEAGVPVVTLAVGVWDTH
                     VRHFPYQRDNVPLLDRSLHALVTDLAQRGLDQDVAVVVCGEMGRTPRINLTAGRDHWP
                     SAGFALFAGGGLRLGQVVGATDARGERPNSRPYSPQNLLATLYHVLGIDPTLTFPDHT
                     GRPRYLLDDQEPIAELV"
BASE COUNT          885 a         1490 c         1569 g          861 t
ORIGIN      
        1 gttcgcctgg tggctggcat ggctcgcgat cctggcgacc ggagtcgtgg gcaactatct
       61 cgacggcagc gctgtcacgc tggtcgatta ctcgatggcc atgcggtgcg acatactttt
      121 ctgtctctcc atcatcgtgg ccggcgtgct ggcgatctac ctgatggtcc ggatcagcca
      181 gcggcagtgg cagcgatacc tggcagtcgc ccacgatgaa tagcgattcg ccaaagccca
      241 gactcaaccc atcagttgat ctcaccgata tcgcggacgc tgaccagctt caggcggccg
      301 aaaggattgc gcattgcgcc gcgtagacgt tcggaagggc tgtcctgcac gatgcactga
      361 aactgcggtc catcgagatc ggccaacacg tgaccgttgg cgaccggctt gtcgccgcga
      421 gcgaggtagc ggatgacttc ggtgatgtcg aaggcgtcgc agtcagcttt tttcatcacc
      481 acctcgcgca ggcccagggc gtgcatgccc atcgtccaga cttccgacgc attgccgatg
      541 atcgccacgt aggcgaagct gattgcgtcg gagctgccct cctccgtcat ttcgagccag
      601 atctcgccgc cgtgcgccag gccgctgttg tcgatgaaca cgccggcgcc gccggccttc
      661 accatggctg ctgcggctcg catcatcgcg tgcgcctgct ctaaagaacc acccgagccg
      721 ctcaggatca cgttcacggt atagcgctcg actctctcta gttcctcgtc ggttggcggg
      781 cggcggcacg acgagcagaa gatctcggca aactgtccat ctgcctccat tgcgccaaac
      841 tcgacctctg tcccgtcttc caggatcagc gcctcccctg tcatccggca ccccggcggc
      901 agcgcctgca ccagctcccg cgggtgcgtc cacgtgcccg gaatgcggaa gtcgatggtg
      961 atcggttcgg ttgcatccgt catgggcgag tctcctgggc aaaccatggc actgcaggct
     1021 atggagattg aggtgacgtt gatcagggtg tgattccaaa acaggaattc gattctacat
     1081 cgcggcggcg gtcgctttgt ccagaaacgc tacacgcggg ggcaaacaca gccagcgctg
     1141 caacaaccca gaccggcatg ccaattgcac ggtttggccg cttaggcgga attcgactag
     1201 ggagcttcac aatgggacga atggaactac agcctgaaac gctcgagcag attcagcagc
     1261 tgatcgtcgc taatttcgcc ggccgtgatg agttgtacgc cgcggctgat acgctggacg
     1321 acggcgcacg tcgcgatgtt tgtcgccgcc ttgccgaatt cctggccgga catgcggtgg
     1381 aactgcagca gattctcaaa gcgaacggcc aagcgcctgc gggaccagtc gctgtcgatc
     1441 cgattgcaac tgcctttttc gattcggcac agtgcagccg tggccaggtc ggcgtgctcg
     1501 ccgcggctgc ggagcgggag cgtgacctga aggaaggcta tcagcacgcg ctggaaaagg
     1561 tgtcgcatcc gagtgcagag aacgttttgc ggcgacacca gaaagaagtc gaattcgttg
     1621 aacaagtgct ccgcggcatg ggtgcggcgg agatagaagg gcagtagctt ccgtcggcag
     1681 gccgtgtcaa tcggcgagtt gagcggcgat cggcgatccg tctggtgagg atgcgatctg
     1741 gctggcgacc atctgttcgg cttgttcggc ggtcagccgg ccgtaggcga cttcgatcat
     1801 caacaggccg acttcattca ttcccatgag tcccgccaat tctttggccc gctcgtacga
     1861 cgcctcggtg aaaaacttga gcctgtacga caggaagagc gcgacgatcg ccccgatgca
     1921 gaccagcacc ggcacaatcc agccgaaagg cgcggctttg acgtccgcca ggaaaatcat
     1981 cgtcagcacc aaagaggcga tgatcgccac gatgctcccc gcgcggaccc acgccgtcag
     2041 gatcgacttg aagctcatcg gaatcggcat gccgcgaaat ccctccgccg tttgctccag
     2101 caccaggtac gaaccggtgg gactgagcgg caggtagtac acgtgcccga accgtgtcat
     2161 cacgtgaaac ataccgccgg gggcctgatc gaccttgccg gcattgatgg tgccccagac
     2221 gataatcatg gcacgctcct ggcggtcctg cgaatgcaga ccggccccgc caagatagcg
     2281 attcgccgcg cggcgtcaag aaaattccgt caaatcatcg ccaagaatca caatacttcg
     2341 caaccgtcaa caggagatgg attgtcgcgc tactgggcca acggcagctc agaaccactg
     2401 agtgatgatc cgttcgctca tgcgccaaga gcgccggaaa tccatgcctg ccttacgaac
     2461 ggcccgtccc agcgcgctcc caacgatcag aatatccatg ctctttaaca atccaatcag
     2521 tggcgaaaag gcggatcgga tggtgcggct gatcgaactg cagccagaaa gccacgtgct
     2581 ggacgtcggc tgcgggacgg gcgagctgct cctgagaatc gtaggtcagc accgcgcccg
     2641 cggtgtcggc gtggatgtca gcccgcagtg tattaccgcg gcacgaaagg ccgccgcaga
     2701 gcgcagcctc gccgagcggt gcgaattcca cgagttcaac gtccgcgatg tcgaactctg
     2761 ccgcaacacg ttcgatctgg gcatctgcgt cggggcgacg cacgcctttg gcccgggaga
     2821 ggcggcttat cccaactcga tttcatggct gcggcagatg gtgcggcctg gtggatatgt
     2881 gctcatcggc gaaggctatt ggaagcggtc gcccgacgct gaatacctgc aacggttggg
     2941 cgaccctgtc ggaatctacc gcgatcatgc ccagaatgtc tcgttcgcgg agcagtgcgg
     3001 gcttgaggcg ctctatgccg tcaccagcag cgacgacgag tgggatgaat ttgaatggtc
     3061 gcactatcaa cagtcgcgcc gcgcagcgga ggccagcccc gacgatcaac agctcgtagc
     3121 cagactggcc cgcagcatcc gctggcgcga cggctacctg cgctggggac gatcgaccat
     3181 gggattcggt ctatatctct ttcgcgttcc gagggaatcc gtatcagccg aagtaaagtg
     3241 agcggctcaa tcacaccagc tccgcgatcg gctcctggtc gtcgagcagg tagcgggggc
     3301 ggccggtgtg gtcggggaag gtgagcgtgg ggtcgatgcc caggacgtgg tagagcgtgg
     3361 ccaggagatt ttgcggcgaa tacgggcgcg agttgggccg ctcgccgcgg gcgtccgtcg
     3421 ctccgacgac ctgccccagg cggaggccgc cgccggcaaa cagggcgaag ccggcactcg
     3481 gccagtggtc ccgcccggcg gtcagattga tgcgcggcgt gcgccccatc tcgccgcaga
     3541 cgacgactgc tacgtcctgg tccaggccgc gctgcgctaa atcggtaacc agggcgtgca
     3601 gcgagcggtc gagcagcggc acgttgtcgc gctggtaggg gaaatggcgg acgtgtgtgt
     3661 cccacacgcc gaccgccagc gtgacgaccg gcacgcccgc ctcgaccagc cgccgggcca
     3721 tcaggaactt gctcccctcc cagcagcggc gcgggtcggg atcgtcctga tactcgatat
     3781 cgccgccgta gagggcgtgc atggcggcgg gctcgcggct gaggtcgaac gcctcgcgga
     3841 cgcgggccga gctgatcatg tccagggcct gctgcgtgaa cgcgtcctgc ccggccatct
     3901 cgccgcgggc gtcgatttcg ctgcggagtt ggtccagcgt gcccagcagc gtggtccggc
     3961 tggtcaggcg gtcgcgcgtc aagccagcgg tcggcgacag gttctggacc tcgcccatgc
     4021 cggggccgct gtaattgatc tggccgttga tgagcgctcc tggctggtac ggcacgaacg
     4081 gctgatgggc cgcgccgacg tacgaaggct cttcatgccc caccagcacg gggtgcgtgt
     4141 ggaactgggc catgctgacg tacggcggca gggcgtcccg ctggccccgc tcgctgcgga
     4201 cgcggctgac gatcgagccg aacgatggcc gctcggcagt cttgggaaag ccgctgaaaa
     4261 ccaggtgcag gtgatgcccg tcgtcgcgcg gccactgcat gctgcggacg atcgccagct
     4321 tgtcggcgat tgtggcctgc agcggcatca gctccgagat ctcgaagccg gggacgttgg
     4381 tgcgaatcgg gcgaaactcg ccgcggatct cagccggaga ttgcggcttg aggtcgtaca
     4441 tgtcgaggtg gctgggcccg ccattgaggc agaccatgat caccgccttg ttcgccggcc
     4501 gctggcgctg cgaattgcct tcagccgccg cagccgcccg cagccgcagc aggctgggca
     4561 gcgagagcgc cccgcccgcg agtccgatgt gcagaaaatc gcggcggcga aggcgatcgc
     4621 agaaccgatg tttcgagccc caaacggtaa gcatgactgg gctccttcgc aggtgggcag
     4681 cttctcgggc gggctgtggc gggcgggact gcgattttag gggaatcgga ttcagggtgc
     4741 aaactgaatc gcaggcccgc cttggccggg gtgtgaaggc gattttcggt ccagggtcgt
     4801 ctata
//
LOCUS       DASUGB010000121         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_292112, whole genome shotgun sequence.
ACCESSION   DASUGB010000121 DASUGB010000000
VERSION     DASUGB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292112"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1005
                     /gene="motA"
                     /locus_tag="VFV87_02310"
     CDS             145..1005
                     /gene="motA"
                     /locus_tag="VFV87_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX5102615.1"
                     /translation="MLVIVGVIVVMACVLGGFMLSGGNIGALIHPAEILTIAGAAFGA
                     AIVMSPKKVLIDTVKGVIQAMKGSPFNRKSYDELFQAMYELFRLARRDGTLALEPHLS
                     NPHESPIFSKYPSVGKNHHTTKFICGALSPIMDGSVTPEQLPSLLGAELKALEEEHHA
                     PISVITKTADALPGFGIVAAVLGIVITMAHIDGPVHEIGHKVGAALVGTFLGILLSYG
                     MFAPLAVKMEFVSAAEMAYFRTIASLVEAFANQLPPKVALEQARRGLSSEVRPEQEEL
                     EALLKAVDAA"
     gene            1070..1867
                     /locus_tag="VFV87_02315"
     CDS             1070..1867
                     /locus_tag="VFV87_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5102616.1"
                     /translation="MAGKGGGAWKVAYADFVTAMMAFFMVMWITAQNEEVKEAVAQHF
                     QDPFADLRGETGDSSSPRHGREHTSRLGPDEDHPETPGKPRPLRVEVAKGSDTLTGTT
                     IFFAGESAELDQAAKDRLAKLLPVLIGKPQKVEIRGHSSRKPLAGDSPYKSFWELSYA
                     RCLTTMEFLEEKGIAPDRMRLSQAGVYEPEASPAARDSAPLGRVEVHLLDEVSPRFTV
                     SKSDPSSPRARNEPVADEKLKAKAAPGGRADASGHAAHGHAQASGAR"
     gene            complement(1887..>2125)
                     /locus_tag="VFV87_02320"
     CDS             complement(1887..>2125)
                     /locus_tag="VFV87_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102617.1"
                     /translation="GRLLGDLYEARANADYKLNDPKAIRNARDVNFVEDQIKWANDVK
                     SILVQCNVEPIRSAVKAGIEADRQRMASRKPGGT"
BASE COUNT          393 a          667 c          692 g          373 t
ORIGIN      
        1 tgatcctttc agcccgcccg ctcccattct gcgacctacc ttctcgtagg cggtgcgctc
       61 agccgggccg gcagcggtcc ggttgtagcg atcttgcaga cctaggaacc gcgaccacga
      121 gtcgcggctt tgggaggttg ctacatgctg gtgatcgtcg gggtgattgt cgtcatggcc
      181 tgcgtgctgg ggggcttcat gctctcgggc gggaatatcg gtgcgctgat tcacccggca
      241 gaaatcctga cgatcgccgg agcggccttt ggcgcggcga ttgtcatgtc gcccaaaaaa
      301 gtgctgatcg acacggtcaa gggcgtgatt caggcgatga aggggagccc cttcaaccgc
      361 aagtcgtacg acgagctgtt ccaggcgatg tacgagctgt tccgcctcgc acggcgcgac
      421 ggcacgctgg ccctggagcc gcacctttcc aacccgcacg agagcccgat cttcagcaag
      481 tatccatccg tcggcaagaa ccatcacacg accaaattca tctgcggagc cctgtcgccg
      541 atcatggacg gctcggtgac ccccgagcaa ctgccgagcc tgctcggcgc ggagctcaag
      601 gccctggaag aagaacacca tgcgccgatc agcgtgatca ccaagacggc agacgccctg
      661 ccgggcttcg gcatcgtggc ggcggtgctg ggcatcgtga tcacgatggc ccacatcgac
      721 ggaccggtgc acgagatcgg ccacaaggtc ggcgcggccc tggtcggcac gttcctgggg
      781 atcttgctct cgtatggaat gttcgcgccg ctggcggtga agatggaatt cgtcagcgcc
      841 gccgagatgg cctatttccg aacgatcgcc tcgctggttg aggcgtttgc gaaccagttg
      901 ccgcccaaag tcgccctgga gcaggcccgc cgcggcctct cgagcgaagt tcgccccgag
      961 caggaagagc tcgaagcgct gctcaaggcg gtcgatgcgg cgtagtcggt agaacgggtt
     1021 tccaacgacg taggacgggt ttccaacccg tcccggcgga gcaatcaaca tggccggcaa
     1081 agggggaggc gcctggaaag tcgcctacgc ggacttcgtg accgcgatga tggccttttt
     1141 catggtgatg tggatcaccg cgcagaacga ggaggtcaaa gaggcggtcg cacagcattt
     1201 ccaggacccg tttgccgact tgcggggcga aacgggcgat tcaagctctc cgcggcacgg
     1261 gcgggagcac acttcgcgcc tggggcccga cgaagaccat cccgaaacgc ccggcaagcc
     1321 gcggccgctg cgggtggagg tcgccaaggg gagcgacacg ctgaccggca cgacgatctt
     1381 cttcgccggc gaatcggccg agctcgacca ggcggccaag gaccgactgg cgaagctgct
     1441 gccggtgctg atcggcaagc cgcagaaggt cgagattcgc ggacacagct cgcggaagcc
     1501 gctggcgggc gattcgccgt ataagagctt ctgggagctg tcgtacgccc gctgcctgac
     1561 cacaatggag tttttggaag agaaagggat tgccccggac cggatgcggc taagccaggc
     1621 gggcgtgtat gagcccgaag cgagccctgc cgcccgcgat tccgcgccgc tgggccgcgt
     1681 cgaggttcac ctgctcgacg aagtctcccc gcggttcacc gtctcgaaat ccgaccccag
     1741 ttcgccgcgg gcaaggaacg agccggtggc ggatgagaag ctcaaagcga aggcagcgcc
     1801 cggcgggcgc gccgatgcaa gcggacatgc ggcccacggg cacgcccaag ccagcggtgc
     1861 gcgttaggcg tcttgcgcag cgctcactaa gttccgcccg gcttgcgact cgccatccgc
     1921 tgccgatcgg cttcgattcc ggccttcacg gctgatcgta tcggttcaac gttgcactgc
     1981 accagaatcg acttcacgtc gttagcccat ttaatttgat cttcgacgaa attcacgtcc
     2041 ctcgcatttc gtatcgcttt ggggtcgttc agcttgtagt cagcattggc acgtgcctcg
     2101 taaagatcgc ccaaaaggcg acctg
//
LOCUS       DASUGB010000122        12079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_848767, whole genome shotgun sequence.
ACCESSION   DASUGB010000122 DASUGB010000000
VERSION     DASUGB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12079
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848767"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFV87_02325"
     CDS             <1..407
                     /locus_tag="VFV87_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102618.1"
                     /translation="ELRNVVFCERVEQFGWYSEFPRNEFRPKQQVILYVEVENFAAER
                     KGQGYETELQGSYQIFDAGGRIVAERQLPLDRETCRNFRRDYFLAYPIYLPDAIEPGR
                     YRLELTIEDLKASGDYQGRKFGEGMIEFTIRQ"
     gene            424..840
                     /locus_tag="VFV87_02330"
     CDS             424..840
                     /locus_tag="VFV87_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5102619.1"
                     /translation="MAELPWYHEGLQFTCTQCGDCCTGAPGHVWVTNDEIAALAAAVG
                     EEVEAFEDKYVRRVGARKSLKEFPNGDCVLLDGKTRRCTVYEARPRQCRTWPFWDSNL
                     KTPEDWKQTCEVCPGSGQGRLYTFEEIELQRKVVKV"
     gene            1260..1616
                     /locus_tag="VFV87_02335"
     CDS             1260..1616
                     /locus_tag="VFV87_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5102620.1"
                     /translation="MTKKEIVKTISEEIGMTQLKTKEIVQKTFNAIIEALVEEPYGRI
                     ELRNFGVFEVKKRAARKARNPRTGERVDVPEKFVVTFKPGKEMEEKVRELEEKYLRGG
                     DSGASARMQASEPPAA"
     gene            1725..1943
                     /locus_tag="VFV87_02340"
     CDS             1725..1943
                     /locus_tag="VFV87_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102621.1"
                     /translation="MRRFILGTLLSLALFANVGCFLPIYSGDPAIRTQELLFTSEDFR
                     NVLEEWRRIWFVDQPSHMTVMRTHGGVL"
     gene            2090..3028
                     /locus_tag="VFV87_02345"
     CDS             2090..3028
                     /locus_tag="VFV87_02345"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX5102622.1"
                     /translation="MTSVPSSGIHTGDLRVQLGRLALRNPILAASGTFGYAREMARLV
                     DFSRLGGILPKTVTAAPRPGNQPWRTVETACGLLNSIGLDNDGIDAFIEHHLPYLREL
                     PTAIIVSIAGRTHEEFVEMAARLGDQPGVAAIELNISCPNVSGGVDFGTDAALCHRLV
                     ESSRKACAVPIIAKLTPNVTSIATIARAAAEGGADAISLINTVLGMAIDWRNRKPLLG
                     NGMGGLSGPAIKPIALRCVYQVAQAVKTPLIGIGGIATIDDVMEFLVAGATAVQIGTA
                     NYYDPTVSMKLADALPAALAAAGAASVKDVVGTLRR"
     gene            3033..4001
                     /gene="trpS"
                     /locus_tag="VFV87_02350"
     CDS             3033..4001
                     /gene="trpS"
                     /locus_tag="VFV87_02350"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5102623.1"
                     /translation="MRVLSGIQPTARFHWGNYFGAIRQYIDLQHGNESYYFIANLHAL
                     TTVREPDALRQNTLDAALDLLALGLDPEKAILFVQSDVPEVAELTWLLMTITPMGLLE
                     RCVSYKDKKDRGIAADAGLFTYPVLMSADILAYDSNIVPVGADQIQHIEVCRDLAGSF
                     NHRFGETFVLPQGKVLESSAKVPGTDGEKMSKSYNNTIEIFEEPKAMRKKVMRITTDS
                     RPVEEPKPNYQEDHLYQLYSLFASDAERAEMAALYQRGNFGYGTVKTALADAAEKYFA
                     EARQRRAELAANPQRIDQILAAGAARARQKAAEVLLRAQKACGIRR"
     gene            4130..5575
                     /locus_tag="VFV87_02355"
     CDS             4130..5575
                     /locus_tag="VFV87_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102624.1"
                     /translation="MFPRSLALAVVCSITLAAAAQTALPSEPVEIGTQPQLFVDQYIV
                     DNTWTLRYKTQHIDRVFHAPEKHLQNPLIKGAAGYVCIAHDAAVGQYHLWYQTHIAGD
                     DEAKTQYAIAYARSQDGLDWELPSLGLHEWNGSKDNNVVIRGKRGRASSPWLLDVPVK
                     DRRGYKYLLMYRDADGSHLVGTNDGVHFDPASDQQIQHLHSDTQNAIVYDPHQRQYVM
                     YCRAKAVYRAGQTEMIDTGESRRVARIANQELWSQWPRQAANILLPDELDTKERYHAF
                     YGMPTTVYAGTFFGFVWPFRWNDRIHTELAWSRDGIHFERHPLRPKLIDYGPPGAWDD
                     EMVFASSWAEVGDEWRIYYAGWDGPHNTRERTGGIGLATLRKEGFVSLRGPAGGGVVC
                     TRVLTWPGGKLLVNATTGPAGQLTVRISDADRKVLPGFDYADCVPFTGDSTAVQIAWK
                     SASIEDLQGQDLRLEIFIQDADLYSFRATGE"
     gene            5861..8980
                     /locus_tag="VFV87_02360"
     CDS             5861..8980
                     /locus_tag="VFV87_02360"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102625.1"
                     /translation="MKRVVAVVTLALLANVALAQNPDEQLFLDPIDVNVPQIRTDKAV
                     AYDYDIVYVRADRAGDEKHKRFFTDFSQPVTIEPGADLMLLHPDGSEEVLIEGGDGSV
                     TDPMISLDGEWVFYSYLYDLRDRNQWDPPPAGADIFKLHLPTRKIVKLTTQQFTPNTG
                     AAPWSSDYRTNEEGKTHFHYGVFNMGPCPLPGGKLAFTSNRDGFRPSKGYPAVALQLF
                     VMNDDGTNIQKIGHLNIAGALHPVLLKDGRLMFSTLESQGIRSEISWGIWTIHPDGTQ
                     WNPLVSAFDPGGASNGFHFQTQLADGHIIIEEYYNQNNSGFGAYIKLPPSPPDGYAAF
                     GPAYRNDPRNSPWKFGRFDNGKPKNYRLPFSPTGSVSFTPFTLNNEGPADRSIIGDKN
                     SPAVGKFTHPSAAPDNHCLTCYSPGPVNHQYTFLPQLDGGIYLIKDGAIVHQPADMRL
                     IKNDPNYNECWPRAVVPYKRIYGEEPTQLEPLANDGSLSPHLPAGTPYGLVGTSSFYK
                     RESYPNGRVPEGKVTSEFAGGNDPWKDLDAFTSHGNGMPVAWHNQGGDVGLYSNDDIH
                     AVRILVMEPTTDRQRGEKSGRLFHSHAKERLRILGEIPLRKFNGDDQPLDPDGNPDTS
                     FLARIPADTAFTFQTLDKNGLMLNYAQTWHQLRPGEIRHDCGGCHAHSQKPTDFALTA
                     AAKPDYQLWDLTRETPLLASKAATSGRGSHTNPKRERGSDEKYDAANSTGLNYIDNGP
                     LNVEYHRDIAPILKRSCAACHSGDEPAAQLNLAADSEEIQVDHHGKFPGTYTRLAMDE
                     RAKFGIKPVRYDSWGYPQASRYIRQFQARRSLLVWKIFGQRLDGFSNDDHPSESKPGA
                     GDVVHQGEKLDPQRFASRMDIDFVGSQMPPEAAVKEGKVQPLTDEDRRTILRWIDLGC
                     PIDLDYDPAHPEDRGYGWMLDDNRPTLTLTLPHEHQAKPLDRILIGMADYYTGLDPAS
                     FTVTADFDVNGIKSGENLAPKFKEIAQGVWELELDQPLKDIAAANIKVSVRDEQGNTT
                     TIDRRFGVQ"
     gene            9126..10211
                     /locus_tag="VFV87_02365"
     CDS             9126..10211
                     /locus_tag="VFV87_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX5102626.1"
                     /translation="MSASGLSIRKDGELDFLSLGALVHRLDPGVIPFRKATRCDIHVS
                     GGEFNVAANLADCFKLKTGVATAMVDYPIGELIAERVRAMGVRPFYKEFKHDGVTGPN
                     MATVYSDQGKGVRAPVVFYNRCNEAAAQLKKGDFDWKKIMSGGLRWFHSGGIFAALSP
                     TTPHLIIEGMQAAKAAGAVVSFDLNFRAKLWQISGGLDKAQETMRRIVANVDVLVGNE
                     EDLQKGLGLKGPDVHSTKQLDPTAFFGIMDQVAKELPNIKVVATTLREVHTTNRHDWS
                     AVAWVNGKTYQAPECPLDVYDRVGGGDGFAAGFFYGLLTGASPEEAIRLGWSHGAMIT
                     TTPGDTTMVTLEQVQAFAKGGSARIQR"
     gene            10276..10530
                     /locus_tag="VFV87_02370"
     CDS             10276..10530
                     /locus_tag="VFV87_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5102627.1"
                     /translation="MQAVTLSQAQQDLSGLISHVVDDAEPVIISTDSGKQVVCLSLDD
                     FNAWKETVYLLASPANAAHLRKSIEETRSGKVEDRSLVQP"
     gene            10527..10784
                     /locus_tag="VFV87_02375"
     CDS             10527..10784
                     /locus_tag="VFV87_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX5102628.1"
                     /translation="MRLVFTPSAWDDYLWFQNHDRKLLKRLNQLIEDVKRSPFQGIGK
                     PEPLKGELSGFWSRRVNDEHRLVYFATATEIVIVACRYHYQ"
     gene            11115..11642
                     /locus_tag="VFV87_02380"
     CDS             11115..11642
                     /locus_tag="VFV87_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102629.1"
                     /translation="MILRQGRFGTNTAVGRAMEELRFEHDDDFATQLHHDHREDVWIG
                     GPYTEVVYLLSCLTRVNYHRTPEKVWYPAAQEFISAHVPKASWITRSHYFAEFAVLLL
                     KDLYRQNGLAKRYPIRAERREERARRFALTNPEMTVEEIAVKLKTTVKQLQRNSSLQV
                     ARVQFERLKNQNSKV"
     gene            11651..>12079
                     /locus_tag="VFV87_02385"
     CDS             11651..>12079
                     /locus_tag="VFV87_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5102630.1"
                     /translation="MPATADIGLIGLAVMGENLALNIESRGYTIAVFNRTVEKVDHLI
                     QGRAKGKKFVGCHSIEELVAAVKTPRKIMMMVKAGQPVDELIEQLLPHMSPGDVLIDG
                     GNEYYLNTERRTKYVESKGLLYIGTGVSGGEEGALKGPSLM"
BASE COUNT         2518 a         3844 c         3591 g         2126 t
ORIGIN      
        1 ttgagctgcg gaatgtcgta ttctgcgagc gggtcgagca attcggctgg tactccgagt
       61 tcccgcgcaa tgagttccgc cccaagcaac aggtcatcct gtacgtcgag gtcgagaact
      121 tcgctgccga gcgaaagggc cagggctacg agacggagtt gcaaggctcg tatcagatct
      181 tcgacgccgg cgggcgaatc gtggccgagc gccagttgcc gctggaccgc gaaacttgcc
      241 gcaattttcg gcgggattac ttcctggcct atccgatcta tctgcccgat gcgatcgagc
      301 ccgggcgata tcggctggag ctgacgattg aggatttgaa agccagcggc gactatcagg
      361 gccgcaagtt tggggaaggg atgatcgagt ttacgattcg gcagtagtcg gtagaatggc
      421 cgcatggctg agctgccttg gtatcacgaa ggtttgcaat tcacctgcac gcagtgcggc
      481 gattgctgca cgggcgcccc tgggcacgtg tgggtgacca acgacgagat tgccgccctg
      541 gcggctgcgg tcggggaaga ggtggaggcg ttcgaagaca aatatgtgcg gcgagtcggc
      601 gcacgcaaga gcttgaagga gttccctaat ggcgattgcg tgctcctgga tggaaagact
      661 cgccgctgca cagtttatga ggcccggccg cgccagtgcc gcacctggcc gttttgggat
      721 tccaacctga agactccgga ggactggaag cagacctgcg aggtttgccc cggtagcggg
      781 cagggccggc tgtacacctt cgaggagatc gagttgcagc gcaaggtcgt caaggtttga
      841 tccgaatatg cggattagcg aaaacagagc gactttgcgg atttagtgga gtttgtggtt
      901 ggaatggtcg attcaaggac gagaaccgcg cagccatagg agcgcaggct caatattgcg
      961 tcgcggttat gatcgacgtt aactcaagtc actaaatgag ttacggcaag gatgccgcac
     1021 cggatgccgc tcttcgccgt aaacaatgag agagtaacgg tttacgacag aaggtacgaa
     1081 cgaaccgtca gatccttgat tgccaagaaa tcgacgtaag tcctgattgt tcgtcccagc
     1141 aaaatccgcc tgagcttgac tctagtgctt gtggcatcta cacttgggtc gctggctggc
     1201 tttcagcaag ccaaacgcga ctgagcgtcg ttccgccact cctggaggag agccgactcg
     1261 tgaccaagaa agagatcgtc aaaacgattt cggaagagat cgggatgacg caactgaaga
     1321 cgaaggagat cgtgcagaag acgttcaacg ccatcatcga agccttggtg gaagaacctt
     1381 acggacgaat tgagctgcgg aacttcggcg tgttcgaagt gaagaagcgg gcggctcgaa
     1441 aggcgcgcaa ccccaggacg ggggagcggg tcgatgtgcc ggagaaattc gtcgtaacct
     1501 tcaagcctgg caaggagatg gaggaaaagg tgcgggagtt ggaggagaag tacctccggg
     1561 gaggcgatag cggtgcatcc gcacggatgc aagccagcga gcctcccgcc gcctaatcga
     1621 gcgattcgca cgaaccatcg gcagcaaaac aagttacgtc gcgtaagcag attgcggcca
     1681 acaaggcgaa cgaccgccag cggcctttca tggaggaaag tgcgatgcgc cggttcattc
     1741 tcgggactct gctcagtttg gccttgttcg ccaacgtcgg ttgctttctc ccgatctatt
     1801 cgggcgatcc ggccattcgg acgcaggagt tgctcttcac gtccgaagac ttccgcaacg
     1861 ttcttgagga atggcgacgg atctggtttg tagatcaacc gagccatatg acagtcatgc
     1921 ggacgcatgg cggcgtcctc taggcgcaac tgcggtctca gcaagagctt gagacgcgcc
     1981 cctcccggcg attgccggcg cagcgcggtg ctggtagatt tgcgctagct ttggtctacc
     2041 ttcgcagctt tcccagacag cccgtgtcgc tccaagtggc tgccctggca tgacttccgt
     2101 tccttctagc ggcattcaca ccggcgatct ccgcgttcag ctcggccgct tggcgctccg
     2161 caatccgatc ctggccgctt cgggcacgtt cggctacgcc cgtgagatgg cacgactggt
     2221 cgatttctcg cggctcggcg gcattctgcc caagacggtc accgcggcgc cgcggcccgg
     2281 caaccagccc tggcggacgg tcgaaacggc ttgcggcctc ttgaattcca tcggcctgga
     2341 taacgacggc atcgacgcgt ttatcgagca ccatctgccg tacctccgcg agctgccgac
     2401 cgcgatcatc gtcagcatcg ccggccgcac gcacgaggaa ttcgtcgaaa tggcggcccg
     2461 cctgggcgat cagccgggcg tggcggccat cgagctgaat atctcgtgcc cgaacgtctc
     2521 cggcggcgtc gatttcggca ccgacgcggc cctgtgccac cggctcgtcg aatcgtcgcg
     2581 caaagcgtgt gcggtcccga tcatcgccaa gctcacgcct aacgtcacca gcatcgccac
     2641 gatcgcccgc gccgcagccg aggggggcgc ggatgccatt tcgctgatta acaccgtgct
     2701 cggcatggcc atcgactggc ggaatcgcaa gccgctcttg ggcaatggaa tgggcggcct
     2761 gtcgggtccg gcgatcaagc cgatcgccct gcggtgcgtt tatcaggttg cccaggccgt
     2821 gaaaacgccg ctgatcggca ttggcggcat tgcgacgatc gacgacgtga tggaattcct
     2881 ggtcgccggc gcgaccgccg tccaaatcgg caccgcgaac tactatgatc caacggtgtc
     2941 gatgaagttg gccgatgcct tgcccgccgc cctcgccgcg gccggcgctg ccagcgtcaa
     3001 agacgtcgtg ggaacactca gaaggtaaag ccatgcgcgt tctctccggc atccaaccga
     3061 cggcccgctt ccactggggt aactacttcg gcgcgatccg ccagtacatc gacttgcagc
     3121 atggcaacga gtcgtactac ttcatcgcca acctgcacgc cctgacgact gtccgcgagc
     3181 ccgacgctct gcgccaaaac acgctcgacg ccgccctcga cctgctcgcc ctcggcctcg
     3241 atccggagaa agcgatcctg ttcgtccagt ccgacgtgcc tgaggtcgcc gagttgacct
     3301 ggctgctgat gacgatcacg cccatgggcc tcctcgagcg ctgcgtctcg tacaaggaca
     3361 agaaagaccg cggcatcgcg gccgatgcgg gcttgttcac ctatcccgtg ctgatgtcgg
     3421 cagacatcct ggcctacgac tcgaacatcg tgccggtcgg cgccgaccag atccagcaca
     3481 tcgaggtctg ccgcgacctg gccggcagct tcaaccaccg cttcggcgag acattcgtcc
     3541 tgccgcaggg caaagtgctc gaaagctcgg ccaaggtccc cggcaccgac ggcgagaaga
     3601 tgagcaagag ctacaacaac acgatcgaga ttttcgaaga gcccaaggcg atgcggaaaa
     3661 aggtgatgcg aatcacgacc gactcgcgtc ccgtggaaga gcccaagccc aactaccagg
     3721 aagaccacct ctatcagctc tattcgctgt tcgccagcga tgccgagcgg gccgaaatgg
     3781 ccgccctcta tcagcggggc aacttcggct acggcaccgt caaaacggct ctggccgacg
     3841 ccgcggaaaa gtacttcgcc gaggcccgcc agcgccgcgc cgagctcgcc gccaatcccc
     3901 agcgcatcga ccagatcctc gccgccggcg ccgcccgcgc ccgccagaaa gccgccgaag
     3961 tcctcctccg cgcccagaaa gcctgcggca tccggcgcta aagtggtccg ccgcgacatc
     4021 gaagcccgca gcgcaagcga gggtaatccg ccttccactc gcaataccct cgcttgcgct
     4081 acgggctaag atggtctgca ccgcaatgtt gcatcctgga ggccgcctca tgtttcctcg
     4141 ttcgctagcg ctcgccgttg tttgctcgat aactctagcc gccgcagctc agacggcgct
     4201 gccgtccgag cccgtcgaga tcggcacgca gccgcagttg tttgtcgatc agtacatcgt
     4261 cgataacacc tggacgctgc gctacaagac gcagcacatc gaccgcgtgt ttcacgcacc
     4321 ggaaaaacat ctgcaaaatc cgctgatcaa gggcgctgcg gggtatgtgt gcattgccca
     4381 cgatgccgca gtgggccagt accacctttg gtatcagacg cacatcgcag gcgacgacga
     4441 ggcgaaaacg caatatgcga tcgcgtacgc tcgctcgcag gacgggctcg attgggagtt
     4501 gccgagcctg ggtcttcacg agtggaacgg ctcgaaggac aacaacgtcg tcattcgcgg
     4561 caagcgcggc cgcgcgagca gcccgtggct gctcgacgtg cccgtgaagg accgccgcgg
     4621 ctacaaatac ctgctgatgt accgcgacgc cgacggctcg cacctggtgg ggacgaacga
     4681 cggcgtccac ttcgatccgg ccagcgacca gcagatccag cacctgcaca gcgacacgca
     4741 aaacgccatc gtctatgacc cgcaccagcg gcaatacgtg atgtactgcc gggccaaagc
     4801 cgtctatcgc gccggccaga cggaaatgat cgacacgggc gaatcgcggc gcgtggcccg
     4861 catcgccaac caggagctct ggagccagtg gccgcggcag gcggccaaca tcctgctgcc
     4921 ggacgagctc gacacgaaag agcgctacca cgccttctat ggcatgccga cgaccgtcta
     4981 tgccggcacg tttttcggct tcgtctggcc gttccgctgg aacgaccgga ttcacaccga
     5041 gctggcctgg tcgcgcgacg gcatccactt cgagcggcat ccgctgcggc ccaagctgat
     5101 cgactatggc ccgccgggag cgtgggacga cgaaatggtc tttgccagct cgtgggccga
     5161 ggtgggcgac gagtggcgga tttattacgc cggctgggac ggcccgcaca acacccgcga
     5221 gcgcaccggc ggcatcggcc tggcgacgct ccgcaaggaa ggcttcgtct cgctccgcgg
     5281 gccggccggc ggcggcgtcg tttgcactcg cgtcctcaca tggccgggcg gcaagctgct
     5341 cgtgaacgcc accaccgggc cggcaggcca gctcactgtc cgcatcagcg acgccgatcg
     5401 caaagtgctc cccggcttcg actatgccga ctgcgtcccc ttcaccggcg acagcaccgc
     5461 cgtccagatc gcctggaaat ccgcgagcat cgaagacctg caaggccaag acctgcggct
     5521 ggagatcttc atccaggacg ccgacctgta cagctttcgt gcaacaggcg aatgaagtgc
     5581 agtgtcactg ggataccgcc gatgcgggac gtgttcgcct gctgaccgac ttccaacctc
     5641 cgtaacgatt ggctggctta gcacatagac gtatgatgca gccggcgtct tagccgggaa
     5701 caaagctggt cccggcgaaa cgtgtggcag gcttccagcc tgccagcact cggcgcggat
     5761 ggcaggcaag tcgcccgccc cactttaaca ggtaagatgc ccgccccact ctcgggagca
     5821 ggtttgctcc cggctaagaa catcgcattg gagtccggcc atgaagcgcg tcgtcgccgt
     5881 tgtcactctt gcacttctgg caaacgtggc cctcgcccag aaccccgacg agcagctctt
     5941 cctggacccg atcgacgtga acgtgccgca gatccgcacg gacaaggcgg tcgcttacga
     6001 ctacgacatc gtgtatgtgc gggccgaccg ggcgggggat gagaaacaca agcggttctt
     6061 caccgatttc tcgcagccgg tgacgatcga gccgggggcc gacctgatgc tgctgcaccc
     6121 ggatggtagc gaggaggtgc tcatcgaagg gggcgacggc tcggtgaccg atccgatgat
     6181 ctcgctcgac ggcgaatggg ttttttactc gtatctctac gacctccgcg atcgcaacca
     6241 gtgggacccg ccgccggccg gcgcggacat cttcaagctg cacctgccga cgcggaagat
     6301 cgtcaaactg accacgcagc agttcacgcc gaataccggc gcggccccgt ggtcgagcga
     6361 ctaccgcacg aatgaagaag gaaagacgca cttccactac ggtgtcttca acatgggtcc
     6421 ctgcccgctg ccgggcggga agctggcgtt caccagcaac cgcgatggct tccggccgtc
     6481 gaagggctat ccggcggtcg ccctgcagct cttcgtgatg aacgacgacg ggacgaatat
     6541 ccagaaaatc ggacacttga acatcgccgg cgcgctgcat ccggtgctgc tcaaggacgg
     6601 gcgcctcatg ttcagtacgc tcgaatcgca aggcatccgc agcgagattt cctggggcat
     6661 ttggacgatc catcccgacg gcacgcagtg gaatccgctc gtcagcgcct tcgaccccgg
     6721 cggcgcgagc aacggcttcc atttccagac gcagcttgcg gatgggcata tcatcatcga
     6781 ggagtactac aaccagaaca acagcggctt cggagcctat atcaagctgc cgccctcgcc
     6841 gccggatggg tatgcggcgt tcggtccggc ttacaggaac gatccgcgga actcgccgtg
     6901 gaagttcggc cggttcgata acggcaagcc gaagaactac cgcctgccct tttcgccgac
     6961 cggcagcgtc tcgttcacgc cgttcacgct gaacaatgaa ggtcccgcgg accgctccat
     7021 cattggcgac aagaattcgc cagccgtcgg caagttcacg catcccagcg ccgcgccgga
     7081 caatcactgt ctcacctgct actcgcctgg cccggtcaac catcaataca cgttcctgcc
     7141 gcagctcgac ggcggcattt acctgatcaa ggacggggcc atcgtccatc agccggccga
     7201 catgcggctg atcaagaacg acccgaatta caacgaatgc tggccgcggg ctgttgtgcc
     7261 gtacaagcgc atctacggcg aggagccgac gcaacttgag ccgctggcca acgacgggag
     7321 cctttcgccg cacctgccgg caggaacgcc ctacggcctc gtcggcactt ccagcttcta
     7381 taaacgcgag agctatccca acggccgcgt gccggaggga aaagtgacca gcgaattcgc
     7441 tggcggcaac gatccgtgga aggacctcga tgccttcacc agccacggca acggcatgcc
     7501 ggtcgcctgg cacaatcagg ggggcgacgt cggcctctat tccaacgacg acattcacgc
     7561 ggtccgcatc ctcgtcatgg aaccaaccac cgaccgccag cgcggcgaaa aatccggccg
     7621 cctctttcac agtcacgcca aagagcggct gcgtatcctc ggggagattc ccttacgcaa
     7681 gttcaacggc gacgatcagc cgctcgatcc cgacggaaat cccgatacaa gcttcctggc
     7741 aagaattccg gccgatacgg ccttcacgtt ccagacgctc gacaagaacg gcctgatgct
     7801 caactatgct cagacctggc accagcttcg ccccggcgaa atccgccacg attgcggcgg
     7861 ctgccacgct cacagccaga agccgaccga tttcgctctc accgccgccg ccaagcccga
     7921 ctaccagctc tgggacctca cccgcgaaac gccactgctc gcttccaaag ccgcgaccag
     7981 tggtcgcggt tcccacacca acccgaagcg tgagcgaggg agtgacgaaa agtacgacgc
     8041 cgccaattcc accggcctca actacatcga caacggcccg ctcaacgtcg aataccatcg
     8101 cgacatcgcc ccgatcctga aacgctcctg cgctgcttgc cactccggcg acgagcccgc
     8161 cgcccagctc aacctggcag cggacagcga agaaatccag gtcgatcacc acggcaagtt
     8221 ccccggtacg tacacgcgtc tcgccatgga cgagcgggcc aaattcggca tcaagccggt
     8281 tcgctacgac tcctggggct acccgcaggc ttcgcgctac atccgccagt tccaggcccg
     8341 ccgcagcctg ctcgtctgga agattttcgg ccagcgcctc gacggattct cgaatgacga
     8401 tcatccttct gaatccaagc cgggagccgg cgacgtcgtc caccagggcg aaaagctcga
     8461 tccccagcga ttcgcctccc ggatggacat cgacttcgtc ggcagccaga tgccgccgga
     8521 agccgccgtc aaggaaggca aggtccagcc actcaccgac gaagaccgcc gcaccatcct
     8581 ccgctggatc gacctcggct gtccgatcga cctcgactac gatcccgccc atcccgaaga
     8641 ccgcggctac ggctggatgc tcgacgacaa ccgccccacc ctcacgctca cgctgccgca
     8701 cgagcatcaa gctaagccgc tcgaccgcat cctgataggc atggcggatt attacacggg
     8761 cctcgacccg gccagcttca ccgtcaccgc cgacttcgat gtaaacggaa tcaaatctgg
     8821 cgagaacctc gcgccaaagt tcaaggagat cgcccaaggc gtctgggagc tggaacttga
     8881 ccagccgctg aaggacattg ccgctgcgaa catcaaggtc agcgttaggg acgagcaagg
     8941 aaacacgacg acgatcgacc ggcgatttgg cgtgcagtaa ccccctttag ccgggaccaa
     9001 acctggtccc ggagaaacgc caaaaacgtc acccgcagtt ctccgggagc tattagctcc
     9061 cggctaggaa cagtaaaatc gccattcgtc gatttctact gctccccgct cgctgatctc
     9121 tccccatgtc tgcttccggc ctgtcgatcc gcaaggatgg cgaactcgat tttctctcac
     9181 tcggcgccct ggtccatcgg ctcgatccgg gcgtgattcc gttccgcaag gcgacgcgct
     9241 gcgacatcca cgtcagcggc ggcgaattca acgtcgcggc caacctggcc gattgcttca
     9301 agctcaagac gggcgtggcg accgcgatgg tcgattaccc gatcggcgaa ctgattgccg
     9361 agcgcgtgcg ggcgatgggc gtgcggccgt tctacaaaga gttcaagcac gacggcgtca
     9421 ccggtccgaa catggccacg gtctatagcg accagggcaa aggcgtgcgg gcgccggtcg
     9481 tcttctataa tcgctgcaat gaagcggcgg ctcagctcaa aaagggcgat ttcgactgga
     9541 aaaagatcat gtccggcggc ctccgctggt tccatagtgg aggcatcttc gccgccctct
     9601 cgccgaccac gccccacctg atcatcgaag gcatgcaagc cgccaaagcc gccggggcgg
     9661 tcgtctcgtt cgacttgaac ttccgggcca agttgtggca gatttccggc gggctcgata
     9721 aggcccagga aacgatgcgg cggatcgtcg ccaacgtcga tgtgctcgtc ggcaacgagg
     9781 aagatttgca gaagggcctc ggcctcaaag gccccgacgt gcatagtacg aagcagctcg
     9841 acccgaccgc cttcttcggc atcatggacc aggtcgccaa ggagctgcct aatatcaagg
     9901 tcgtcgccac gacgctccgc gaggtccaca ccacgaaccg ccacgactgg agcgccgtcg
     9961 cttgggtgaa cgggaaaacg tatcaggccc ccgaatgccc gctcgacgtc tatgaccgcg
    10021 tcggcggcgg cgacggcttc gcggcggggt tcttctacgg cctgctgacc ggcgcctcgc
    10081 ccgaagaagc catccgcctc ggctggtcgc acggcgcgat gatcaccact acccccggcg
    10141 acaccacgat ggtcacgctc gaacaagtcc aagccttcgc caaaggcggc tcggcccgga
    10201 ttcagcggta gcgagaattc cgcggtagaa tggtgaacag cggatgcgaa tcaacgtccg
    10261 tgcggaggtt aagcaatgca agcggtaacg ctgtcccagg ctcaacagga cctttccggt
    10321 ctcatttctc atgttgttga cgatgccgag cccgtaatca tctccaccga ctcagggaag
    10381 caggtcgtgt gcctctctct cgacgatttc aatgcgtgga aggaaacggt gtacctgctt
    10441 gccagtccgg cgaacgcggc tcatctacgt aagtcgatcg aagagaccag gtcggggaaa
    10501 gtcgaagacc ggagcctggt ccagccatga ggctcgtttt cactccttca gcctgggacg
    10561 actacttgtg gtttcagaat cacgaccgca aactgctcaa acgactcaac caattgattg
    10621 aggacgtgaa acgctcaccc ttccaaggaa tcggcaagcc ggagccgctg aagggtgagc
    10681 tatcgggatt ctggtcgcgt cgcgttaacg acgagcatcg gctagtgtat tttgcgacgg
    10741 cgacggaaat cgtaatagtc gcatgtagat accattatca gtgaatggcc aatcaatgag
    10801 gcatggcatg cctacttccg caccggcggc agatgaacgc gttgccgacg tgtcattccg
    10861 caaagagcag ctcacagtga cattgcgcga cggccgcgcg attttcgtgc cgctggcctg
    10921 gtatccgcgg ctgctcaagg ccaccgcgaa gcagcgcaag aactggctga tcgcgggggg
    10981 gcggctacgg aattcattgg cccgagatcg acgaggactt gaacaccgag ggtctattgc
    11041 gcggggcacc ctcaccggag gcaaccaggc cagcccgccg ttccacttcg tcccgctcac
    11101 aacggcgcgc gtaagtgatc cttcgccaag ggcggtttgg cactaataca gcagtaggtc
    11161 gcgcgatgga ggagcttcga ttcgagcatg acgatgattt tgccacacaa ctgcatcacg
    11221 accatcgaga agacgtctgg ataggtggac catacacgga agtcgtatac ctgttgagtt
    11281 gcctgacgag agtgaactac catcgaacgc cagagaaggt gtggtatccc gccgcgcagg
    11341 aattcatttc tgctcacgtg ccgaaagcta gctggatcac tcgctcacat tactttgcgg
    11401 aattcgcggt gttgctcctc aaggatctct atcgccagaa cggacttgca aagcggtatc
    11461 ccattcgtgc agagcgtcgc gaggaacgag caagacgatt tgccctcact aatccagaaa
    11521 tgactgtcga ggagatagct gtcaaattga aaactactgt caagcaactg cagcgaaaca
    11581 gttcgttgca ggttgcccga gttcagtttg aacggttgaa aaatcaaaac tctaaagtct
    11641 agcatcatcc atgcccgcta ccgctgatat cggattaatt ggtctcgccg tcatgggcga
    11701 gaacctcgct ctcaatatcg aaagccgcgg ctatacgatc gcggtcttta accgcaccgt
    11761 ggaaaaggtg gatcatctga tccaaggccg ggcgaagggc aaaaaattcg tcggctgcca
    11821 ttcgatcgag gaattggtcg ccgcggtcaa gactccgcgg aagatcatga tgatggtcaa
    11881 agccggccag ccggtggacg agctgatcga gcagcttctg ccgcacatgt cgccggggga
    11941 cgtgctgatc gacggcggca acgagtacta cctgaacacc gagcggcgga cgaagtacgt
    12001 cgagagcaag gggctgctct atatcggcac cggcgtcagc ggcggcgagg aaggggcgct
    12061 caaaggcccc agcctgatg
//
LOCUS       DASUGB010000123         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1264766, whole genome shotgun sequence.
ACCESSION   DASUGB010000123 DASUGB010000000
VERSION     DASUGB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264766"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VFV87_02390"
     CDS             <1..1242
                     /locus_tag="VFV87_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5102631.1"
                     /translation="ASAVQVARGANVFGSDLIKVGLVGCGGRGTGATIQALNTSGGAL
                     MVTALADAFADRLQVAYRTIKGQHEDKIIVPKEKQFVGLNAFKELLQSDCDLVILATP
                     PGFRPLHFEAAVAAGKHVFAEKPVAVDAAGVRQFLAASEEAKKQNLAVAIGLQRRHER
                     KYMETIKAIQNGAIGDIVLARAYWNGRKPWWKDRQDNQTELEYQMRNWYNFAWLSGDH
                     IVEQHIHNLDVINWIKNDYPTEAQGQGGCTIRKKEFGEIYDHHFVEFKYGDGTVMMSQ
                     CRHIPDTWQSVSEYVHGTKGYADVSGSKIYAHDGKVMHDFGKLGADGHQQEHHDLFAD
                     LRAGRIAAEGEYGAKSTMTAILGRMATYSGNVVKWDEAINSQLVIAPVAQMNSFDDQA
                     PVKPDAEGWYALPVPGVTKVV"
     gene            1388..1461
                     /locus_tag="VFV87_02395"
     tRNA            1388..1461
                     /locus_tag="VFV87_02395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1422..1424,aa:Arg,seq:acg)
     gene            1575..2795
                     /locus_tag="VFV87_02400"
     CDS             1575..2795
                     /locus_tag="VFV87_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5102632.1"
                     /translation="MAHQKTAVPSYCRHKASGQAVVRLDGVDRYLGPYGSPESYERYE
                     RAIAEWRVHHAEKQTATERLSAVRRFDLTVSEVLFRYRQFALGYYQKNGQPSKELQAV
                     KYSLRPVRLLYGSIRIRDFGPLALKAVREHMVSSGLCRNLVNARINRVKRFMKWAVSE
                     ELAPPSAYEALRTVTGLRFGRSGARETDPVQPVPDAWVNAVLPFLSPQVKAMVSLQRL
                     TGMRPCEVVQMRVGDIDMTGEIWIYEPPDHKNRWRGHKRLIPLGPKSQEIIRPFLSLK
                     TVAFLFSPIAAEAWRNDLRRQRRQTPMTPSQAARRPKSRPKRAKRERYDVDSYRRAIE
                     YGIEVANRQRTEFDQIPHWFPLQLRHSRATEVRKAFGLEGAQVALGHANASVTEVYAE
                     RNLGLAIEIAKRSG"
     gene            2817..>4081
                     /locus_tag="VFV87_02405"
     CDS             2817..>4081
                     /locus_tag="VFV87_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102633.1"
                     /translation="MAAGVADDVVLSALSGEVPFADGSDSSLASRLAAVVDAGRMFAQ
                     LATTAKLAVLHSDDTYSEAALRTAGDLCAVVRRIGASAEERYELEALAVRFLLDIRRE
                     LAPTTIRSRRECAIRLVMDDGDDELQGSPFDERSIGNLAIAMVDAICAEFLIVLSAAL
                     PTEFLAWLDIALHLQTTLYPQYLVELVFPESSQLVYSPSVDPVEAGVGGSWLSTALPS
                     YTVPQRIDLGSEILDYGWLQLTIQRYRELNVMLPDNLRHVESAVTRLAAVESLFTAID
                     NRIGGPPDNAVLPKFAECAVRSETAARHCSGSGEVSKCQQTLARVFRVSIDEQRKILS
                     RDRRETPFPKGNSFLWQLAAFLLSATEGRTTAAIREKWKEIGGKSANPCSRTIENNVS
                     RVNLLLEPLRLEAGRPKHNGPWTIIDLSPR"
BASE COUNT          840 a         1230 c         1246 g          765 t
ORIGIN      
        1 gccagcgcag tccaagtggc ccgcggagcg aacgtcttcg gcagcgatct gatcaaggtc
       61 ggcctggtcg gctgcggcgg tcgcggcacg ggcgcgacga tccaagcgct gaacaccagc
      121 ggcggggcgc tgatggtaac cgccttggcc gacgccttcg ccgaccgcct gcaagtcgcc
      181 taccgcacca tcaagggcca gcacgaagac aagattattg ttcccaaaga gaagcagttt
      241 gtcgggctca acgccttcaa agagttgctg cagtccgatt gcgacctcgt gatcctcgcc
      301 accccgccgg gcttccgccc gctgcacttt gaggcggccg tggcggctgg caagcacgtc
      361 tttgccgaga agcccgtggc ggtcgatgcg gccggcgtgc gccagttcct ggccgccagc
      421 gaagaagcca agaagcagaa tctggctgtc gccatcggcc tccagcgccg gcacgagcgg
      481 aaatacatgg agacgatcaa ggcgatccag aacggtgcga tcggcgacat cgtgttggcc
      541 cgtgcctatt ggaatggccg caaaccgtgg tggaaggacc gccaggacaa tcaaaccgaa
      601 ctcgagtacc agatgcgcaa ctggtacaac ttcgcctggc tcagcggcga ccacattgtc
      661 gagcagcaca tccacaatct cgacgtgatc aactggatca agaacgacta tccgacggag
      721 gcccagggac agggcggctg cacgatccgc aagaaggaat ttggcgagat ttacgatcat
      781 catttcgtcg agttcaagta cggcgacggc accgtgatga tgagtcagtg tcggcacatt
      841 cccgacacgt ggcagtcggt cagcgagtac gtccacggaa cgaagggcta cgccgacgtc
      901 agcggctcaa agatctatgc tcacgacgga aaggtcatgc acgacttcgg caagctgggc
      961 gccgacggcc accagcagga gcatcacgac ctgttcgccg acttgcgcgc cggccgcatc
     1021 gccgctgagg gcgagtacgg cgccaagagc acgatgaccg caatcctcgg ccgaatggcg
     1081 acttactcgg gcaacgtcgt gaagtgggac gaagcgatca attcgcagct cgtcatcgct
     1141 cctgtcgccc agatgaacag cttcgatgac caggctcccg tcaaacccga tgccgagggc
     1201 tggtacgccc tgccggttcc cggtgtgacc aaggtggtgt aggacgtgga gacctgaccg
     1261 cctgctgccc gctggcgtct gcctttgcgc gccggcggag gtccgctaca ttgaaggtgc
     1321 cattgcggcc ggcctccgtc ggaccgaact cgccccggtc ccggcgtgtc ttgccacaat
     1381 cggcagcgca cccatagctc aattggatag agcatcggtc tacggaaccg aaggttggag
     1441 gttcgagccc tcctgggtgt actgatcgta aatcttgaca ccgtttgcct ttagggttcc
     1501 gcataagcgt ttgccgtagc ggctctttgg ctcagattcc tccgtaattt acggagggat
     1561 gggagcggag cgctatggct caccagaaaa ctgcggttcc cagctactgc cgccacaagg
     1621 cctctggcca ggcggtcgtc cgcctcgacg gcgtcgatcg ttatcttggt ccctacggca
     1681 gcccggaaag ttacgagcgg tacgagcgcg cgatcgcgga gtggcgcgtc caccacgctg
     1741 aaaagcaaac cgcaactgaa cgcctttcgg cggtgcgccg ctttgacctt acagtctctg
     1801 aggtgctgtt ccgctatcgc caattcgcgc tcggctatta ccagaaaaac ggccagccca
     1861 gcaaggaatt gcaggccgtt aagtactcgc tgcggcccgt ccgcctcctc tacggatcga
     1921 tcagaatccg agacttcggc ccactggccc tcaaggccgt ccgcgagcac atggtctcca
     1981 gcggcctctg ccgcaatctg gtcaacgccc gaatcaatcg cgtcaaacgc ttcatgaagt
     2041 gggcggtcag cgaagagctc gcgccgccta gcgcgtatga ggcgctgcga acggtgaccg
     2101 gattacgttt cggtcgatca ggagcccggg aaacggatcc ggtccaaccc gtccccgatg
     2161 cctgggtcaa tgccgtcctg ccatttctat cgcctcaggt gaaagcgatg gtcagcctgc
     2221 aacgtttgac gggaatgcgg ccgtgcgaag tcgtgcagat gcgcgttggc gacatcgaca
     2281 tgaccggcga aatttggatc tacgagcccc cggaccataa gaaccggtgg cgcggacaca
     2341 agcgactcat tcctctcggg cccaagtccc aagagattat tcggccattc ctgtcgctga
     2401 agacagttgc attcctcttt agcccaatcg ccgccgaagc ctggcgcaat gacttgcgtc
     2461 gtcagcggcg gcagacgccg atgacgccat cgcaggcggc gcgccgaccg aaatcgcgtc
     2521 ccaaacgagc caaacgtgag cggtacgacg tcgactcgta ccgccgggcg atcgaatacg
     2581 gaatcgaagt ggccaaccgc cagcggacgg aattcgatca gattccccat tggttcccgt
     2641 tgcagctgag gcactcgcgg gccacggaag tgcgcaaggc ctttggcctg gaaggagcgc
     2701 aggtcgcctt gggtcacgcc aacgcgagcg tgactgaggt ttacgccgaa cgaaacctgg
     2761 gattggccat cgagatcgcc aaacgtagtg gctaagcaca agaatcggag gatgccatgg
     2821 ctgccggagt tgcggacgat gtcgtgctct cagcgctgag cggtgaggtg ccgtttgcgg
     2881 atggcagcga ttcctcgctg gcatcgcgat tggccgcggt cgtcgacgca gggcggatgt
     2941 ttgcgcagct ggccacgacg gccaaactgg ccgtattgca cagcgacgac acttacagcg
     3001 aagccgcgct gcgaaccgcc ggtgacttgt gcgccgtggt ccgccggatc ggagccagcg
     3061 ccgaagagcg ttatgaattg gaggcactgg cagtcaggtt tctgctcgac ataagacgcg
     3121 agttggcgcc gacgacaatt aggtcgagac gggagtgcgc gatccggctg gtaatggatg
     3181 acggggacga cgaactgcaa gggtctccct tcgacgaacg cagtattggc aacctcgcga
     3241 ttgccatggt cgatgcaatt tgcgcagagt tccttatcgt cctctccgcc gcgttgccca
     3301 cggaattcct tgcgtggctc gatatcgctt tgcacctaca gacgactctg tacccgcagt
     3361 acttggtcga actcgtgttt cctgaaagtt cacagctggt gtactcaccg tcggtggatc
     3421 ccgtcgaggc gggcgtcggc ggcagttggc tctcaactgc gcttccgagt tacacggtgc
     3481 cccagcggat tgatcttggt tctgagattc tcgactacgg ctggttgcaa ctcacgatcc
     3541 aaaggtaccg cgaactgaat gtaatgcttc ctgacaacct gcgccacgtg gagagcgcgg
     3601 tcacgcgttt agccgccgtc gaatctcttt tcactgcgat agacaatcga attggcggac
     3661 cgccagacaa tgccgtgttg ccaaagttcg ccgagtgtgc cgtgcgttcg gaaacggcag
     3721 cacgccattg ctcgggctcg ggagaggtca gcaagtgtca gcagacgttg gctcgcgtgt
     3781 ttcgcgtgtc aatcgacgaa cagcgcaaga tactttcgcg agaccgaaga gaaactcctt
     3841 tcccgaaggg caattcattt ctttggcaac tggctgcctt tctgttgtcg gccaccgaag
     3901 gcaggaccac tgctgccatc cgcgagaagt ggaaggaaat cggcgggaaa tcggcgaatc
     3961 cttgctcaag gacgattgaa aacaacgtat caagagtcaa tctgctcctg gagcctctca
     4021 gacttgaagc tgggcgtccg aaacacaatg gaccttggac catcatcgat ttgagtccac
     4081 g
//
LOCUS       DASUGB010000124         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_570521, whole genome shotgun sequence.
ACCESSION   DASUGB010000124 DASUGB010000000
VERSION     DASUGB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570521"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFV87_02410"
     CDS             <1..561
                     /locus_tag="VFV87_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102634.1"
                     /translation="LWLDGKRPAALLCLISKGEKWNFEFHSLLEEPLRSSDRGKPNWQ
                     PAAAARAWVAVEGAVPEASRARQLALRGIARRFEASETRQGEKFPLRLQDRPLYTYAD
                     ADNGVIDGGLFAMSYGTNPELLLQIEARKGEDKPEWHVAFARLSAAEVIVQMATKEAW
                     RADAIPPNSYKPADPYYAYNEVAAPE"
     gene            628..1494
                     /locus_tag="VFV87_02415"
     CDS             628..1494
                     /locus_tag="VFV87_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5102635.1"
                     /translation="MTITTLHTQHPRRFEDNRFVYPVLSRRAAGISVGVNLNPDKVCN
                     FDCIYCQVDRTTQSETRFVETAQLLDELEMALKLASSGELFEHPKFRETPPQFRRLND
                     MAFSGDGEPTTYKNFDEIIAACAELKQRLGLDAVQMVLITNASMFHRPHVQCGLAILD
                     QNNGEIWAKLEAGTAEYFKLVDRTPIPFQQILDNITAAAQVRPLVIQALFMRVNGQPP
                     SEAELSAFCDRLNEIAAAGGKLRLVQVYTVARRPAESYVAPLADAEVDAIVSLVHSRT
                     GLAAQAFYGTSP"
     gene            1491..1745
                     /locus_tag="VFV87_02420"
     CDS             1491..1745
                     /locus_tag="VFV87_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102636.1"
                     /translation="MKPDQPAPRPVNLTLWIMVAVLIWGGYLAIGAVRAGGNLPSLRG
                     LIIFVCTLIFLGLWWLAIAFRARRAAAVVQDEEDSGAHSA"
     gene            1742..2137
                     /locus_tag="VFV87_02425"
     CDS             1742..2137
                     /locus_tag="VFV87_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102637.1"
                     /translation="MNDSSPAAMPPPSQIWPLVRGVGGAIAGGAAGYFVFRWLATNGF
                     YGLMIPGLLVGLGAGLAARGRSHVLGVICAIAALMLMIVAEWLRAPMAQDPSLLYFIT
                     HLHQMDGAAVKFLMLAVRTAAAYWFGQGR"
     gene            complement(2156..3577)
                     /locus_tag="VFV87_02430"
     CDS             complement(2156..3577)
                     /locus_tag="VFV87_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102638.1"
                     /translation="MNPLLAARRHFFQQCRMGLGAMALGSLLANDGFAAEATGSDPLA
                     PRAPHFTPRAKNVIFLFMAGGPSQLELFNPKPKLQELGGKVIPQSFVANKRFAFLKAD
                     AKLLGTNRKFEKCGESGQVISECLPHLKTIADDICTLRAVKTDVFNHGPAKFFMNTGS
                     PLVGRPAMGAWVTYGIGSESRDLPGFVVLQSGPRGPRGGSLNWGSGFLPTTYQGVPLR
                     SKGAPILNLENPQGIAAADQERFIRSVNDLNRLRLEATGDGEIATRIAAYEMAFRMQS
                     SAPELMDLSGENAETLKLYGCEPGKPSFAMNCLLARRLVERGVRMVQLYHTDWDHHGN
                     KGTELGESLDKICLETDQGAAALVKDLKQRGLLNDTIVIWGGEFGRTPQGEPREMLGR
                     DHHVESFTMWLAGGGVKPGLSLGQTDEIGYNGTEDVVHVHDLHATILHLLGLDHLQLT
                     YRFQGRDFRLTDVAGNVVQKVLA"
     gene            complement(3581..3943)
                     /locus_tag="VFV87_02435"
     CDS             complement(3581..3943)
                     /locus_tag="VFV87_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102639.1"
                     /translation="MTDAERDRLTALRAAVQAELPDLVARNQLREEARREPTLSGALR
                     RAVRDSHRPLHHLAREIGVEAVQLDEFLTGERRLFSDVMDRLARAVGVELPIAAVPLA
                     NPAARQSIDSAAAHTPGT"
     gene            complement(3976..4227)
                     /locus_tag="VFV87_02440"
     CDS             complement(3976..4227)
                     /locus_tag="VFV87_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102640.1"
                     /translation="MAWQSFLWSDDNIPHIAEHGISPADFEFVMNHPDEYSVSHSSGL
                     PCAFGRTPDGRYIIAVFEEIDDATVYPVTAYEVPEPGHS"
BASE COUNT          748 a         1378 c         1445 g          811 t
ORIGIN      
        1 ctgtggctcg acggcaagcg gcccgccgcg ttgctctgcc tgatctccaa gggggagaag
       61 tggaatttcg agttccattc gctgctggag gagccgctgc gctcgagcga ccgcggcaaa
      121 cccaactggc agccggcggc cgcggcccgc gcttgggtcg ccgtggaggg tgcggttccc
      181 gaggcgtcgc gggcccggca gcttgcgctg cgcgggattg cccgccgctt cgaggccagc
      241 gagacgcgcc agggcgagaa gtttccgctg cggctgcagg accggccgct ctatacctat
      301 gccgacgcgg acaacggcgt catcgacggc ggcctgtttg ccatgtcgta cggcacgaat
      361 ccggaactgc tcctgcagat cgaggcccgc aaaggcgagg acaagcccga gtggcacgtc
      421 gccttcgccc gcctctcggc tgcggaggtg atcgtgcaga tggcaaccaa ggaagcctgg
      481 cgagccgacg cgattcctcc gaacagctac aagcctgccg atccttacta cgcgtacaac
      541 gaggtcgcag cgccggaatg aggcggacat cggtcgcccg cgacggacgc cggtctataa
      601 tgctctgtag ttcgctactg agcaccaatg accatcacca cgctccacac ccagcacccg
      661 cgccggttcg aggacaaccg cttcgtctat ccggtgctgt cgcgccgagc cgcggggatt
      721 tcggtggggg tgaatctgaa tccggacaag gtttgcaatt tcgactgcat ctattgccag
      781 gtggaccgga ccacccagag cgagacgcgg tttgtcgaga cggcgcagct cttggatgag
      841 ctcgaaatgg cgctcaagct ggcgtccagc ggcgaattgt tcgagcatcc aaagtttcgt
      901 gagactccac cgcagttccg ccggctgaac gatatggcct tctcgggcga cggcgagccg
      961 acaacctaca agaacttcga cgagatcatt gcggcttgcg cggaattgaa gcagcggctg
     1021 ggcctggacg cggtgcagat ggtgctgatc accaacgcca gcatgttcca ccggccgcac
     1081 gtgcagtgcg gcctcgcgat cctcgaccag aacaacggcg aaatctgggc caagctcgaa
     1141 gcggggaccg cagagtactt caagctggtt gatcggacgc cgattccctt ccagcagatc
     1201 ctcgataaca tcaccgcggc ggcgcaggtc cgcccgctgg tgattcaggc tctcttcatg
     1261 cgggtcaacg gccagccgcc gagcgaggcg gagctgagtg ccttctgcga ccggctgaac
     1321 gagatcgcgg cggcgggcgg caagctgagg ctggtccagg tctataccgt cgcccggcgg
     1381 ccggcagaga gttatgtcgc cccgctggcg gacgcggagg tcgatgcgat cgtgagcctc
     1441 gtccactcga ggaccggcct ggctgcccag gccttttacg gaaccagccc atgaaacccg
     1501 accagccggc cccgcggccg gtcaatctca cgctgtggat catggtcgcc gtgctcatct
     1561 ggggcggcta cctggcaatc ggcgcggtgc gggccggcgg caacctgccc agcctgcggg
     1621 gactgatcat tttcgtctgc acactgatat tcttggggct ctggtggttg gcgattgcgt
     1681 ttcgcgcccg gcgagccgcc gccgttgtcc aggacgagga agattcagga gcccactccg
     1741 catgaacgat tcatcgccag ccgccatgcc gccccccagc cagatctggc cgcttgttcg
     1801 cggcgtgggc ggcgctatcg cagggggagc ggctggttac ttcgtgtttc gctggttggc
     1861 aaccaatggg ttttacggcc tgatgatccc cgggctgctc gtgggcttgg gagccgggct
     1921 ggcagcgcgc gggaggagcc acgttttggg agtgatctgc gccatcgccg cgctgatgct
     1981 gatgatcgtc gccgaatggc tccgcgctcc gatggcccag gatccgagcc tgctctattt
     2041 catcacgcat ctgcaccaga tggacggcgc cgcggtcaag ttcctcatgc tcgccgtccg
     2101 cacagccgcc gcgtattggt tcggtcaagg gcggtagcga tacgcatcca accacttacg
     2161 ccagcacctt ctgcaccacg ttgcctgcga cgtcggtcag gcggaagtcg cggccttgga
     2221 agcggtaggt gagctggagg tggtcgaggc ccaggaggtg caggatcgtg gcgtgcaggt
     2281 cgtggacgtg gaccacgtct tcggtgccgt tgtagccgat ctcgtcggtc tggccgagcg
     2341 agaggccggg cttcacgccg ccgccggcca gccacatcgt gaagctttcg acgtgatggt
     2401 cgcggccgag catctcacgc ggctcgccct gcggcgtgcg gccgaattcg cctccccaga
     2461 tcacgatggt gtcgttcagc agcccgcgct gcttgaggtc cttcaccagg gctgcggcgc
     2521 cctggtcggt ttccaggcag atcttgtcga gcgattcgcc cagctcggtg cctttgttgc
     2581 cgtggtggtc ccagtcggtg tgatagagtt gcaccatccg cacgccccgc tcgaccagcc
     2641 gccgggccag caggcagttc atcgcaaaac tcggcttacc gggctcgcag ccgtagagct
     2701 tgagcgtctc ggcgttttcg cccgagaggt ccatcaactc cggagcgctc gactgcatgc
     2761 ggaaggccat ttcgtaggcg gcgatccgcg tggcgatttc gccgtcgccc gtggcttcca
     2821 gacggaggcg gttgaggtcg ttcaccgaac ggatgaaccg ctcctggtcg gcagcggcga
     2881 tgccctgcgg gttctccaga ttcaggatcg gcgcgccttt gctccgcagc ggaacgccct
     2941 gatacgtcgt gggcaagaag ccgctgcccc agttcagcga gccgccgcgc ggcccgcgcg
     3001 ggccggattg cagcacgaca aaccccggca aatcgcgcga ctcgctgccg atgccgtagg
     3061 tgacccaggc ccccatcgcc ggccgaccca ccaagggcga gccggtgttc atgaagaact
     3121 tggccggccc gtggttgaag acgtcggtct tcaccgcccg caatgtgcag atgtcgtcgg
     3181 cgatcgtctt caggtgcggt aggcactcgg agatgacctg cccgctctcg ccgcatttct
     3241 cgaacttgcg attcgtgccg agcagcttgg cgtcggcctt cagaaaggca aaccgcttgt
     3301 tggcgacgaa cgattgcggg atgactttgc ctcccaattc ctgtagcttc ggcttcggat
     3361 tgaacagctc gagctggctc ggccctcccg ccatgaacag aaagatcaca ttcttggccc
     3421 gcggcgtgaa gtgcggcgcc cgcggcgcga gcggatcgct gccggttgct tccgccgcga
     3481 agccgtcatt ggcgagcagc gagccgagag ccatcgcacc gagacccatg cggcattgct
     3541 ggaagaaatg gcggcgagcg gcgaggagcg gattcatgcg ctacgttccg ggtgtgtggg
     3601 ctgcggctga atcaatagat tggcgcgccg cgggattagc gagtggaacg gcagcaatcg
     3661 gaagctccac tccaactgct ctagccaggc gatccatgac atcggagaac agcctgcgct
     3721 cgccagtcaa aaactcatcg agctgtaccg cttcgacgcc aatctctcgt gccagatggt
     3781 gcaatgggcg gtggctgtcg cgcacggcgc gccgcagcgc gccgctgagc gtcggttctc
     3841 gccgcgcttc ttcgcgcaac tgattgcggg ctaccagatc aggaagctct gcttgcaccg
     3901 ccgcgcgcag agcggtgagc cggtcgcgtt ccgcgtccgt caagtcccgt ggtcgagagg
     3961 gagtgggtgt cgtcatcagg agtgtcccgg ctctgggact tcgtaggcgg tgaccggata
     4021 gaccgtggcg tcgtcgattt cttcaaacac cgcgataatg tatcgcccat ccggcgtgcg
     4081 tccaaaggcg catggcaatc cggaggagtg gctcacggag tattcatcag gatgattcat
     4141 cacgaattca aaatcggcgg gcgagatccc atgttcggcg atatggggaa tgttgtcgtc
     4201 agaccaaagg aacgattgcc aagccatgca gcaatcctac gattatacgt tgattggggc
     4261 gatttgacgc atcattccct cgtaatcgtc tcatcgagat tcagcagcac gcggcagatg
     4321 cgggtccagg cggcggtcgg ttgttcggat gcgaccgcga gttcgtcggc gacgagttgg
     4381 ct
//
LOCUS       DASUGB010000125         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_570786, whole genome shotgun sequence.
ACCESSION   DASUGB010000125 DASUGB010000000
VERSION     DASUGB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570786"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..836
                     /locus_tag="VFV87_02445"
     CDS             243..836
                     /locus_tag="VFV87_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5102641.1"
                     /translation="MRPTGDNVVQDDSRWIEAARRGDPTAFGQLVRKYQDRLVTALLY
                     VCGSHDEAEDVAQEAFVHAYVKLASFEGASAFYTWLYRIAINAAISRRRRRREEESVE
                     EQRDRLGREPQDGGEGAEERLLREERAVLVRRALGRLSDEHRAILVLREVDGCDYEQI
                     AAILDVPVGTVRSRLHRARLHLKEQLDVVLGKAEQRE"
     gene            873..1847
                     /locus_tag="VFV87_02450"
     CDS             873..1847
                     /locus_tag="VFV87_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5102642.1"
                     /translation="MTHFSDELLSAYLDGELSAEERAAVKSRLAESEADRRLLEELQA
                     LRSELQSLPRAELTSEFTDRVVRAAAESQEHINLQVTPAAADPGAGRTRRFSWPLWTA
                     AVAAGLAICAVLIVRNWPLGDANVVVGPGPIPPTVPSPAELLIAQLRQAAPTQDEAVV
                     IRLRVPKNSPLGPSLDVALAEAGIRQRPAFDLTSGAIELGAAYRKQVQQKPANDLVAV
                     SDALFLNAPLSQIEEALAAIAGADDRALRLAAETRLALADLPKPQEGPEGEPGSENAG
                     GPFSQRLNAGMFRLEKRESAAAWPTDVVPAALNPQQTVKLLILIEQVD"
     gene            complement(1935..3104)
                     /locus_tag="VFV87_02455"
     CDS             complement(1935..3104)
                     /locus_tag="VFV87_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5102643.1"
                     /translation="MATDFLIVGGGIGGLVLAEMLGRGGKRVVVLERSTGPPPWSRPE
                     ILWPTTLDLLCTLIPRTRWDEAIVPLAGVELFDGHEFQWLISPDALRRAQVHPWSTNP
                     NLTRELLMRLESFELHRGVEVKEILKNKDRIIGARSRDVATGQEREWLADWTVGDDGG
                     HSLVRQACGIDLKTRLFPLDLLCFGGDWPAEFRPNAGRVWPNYRSARSGIMAAGCLPT
                     AQRQAVGIVPVRPRIFDDVPRAQAAWEEFIEMDPSLKSVVGQRKFPDDFMRVRRPWGH
                     APRYGGAGALIMGDAAHPVSPAGGQGANASIADGRAIAELALAGERDLLTAYERRRRP
                     ANRRSLRFTSGASRLFNLPHRLAFNRFTAGIARQVGRQPQLVARLVRMVSRAFVG"
BASE COUNT          549 a         1027 c         1033 g          507 t
ORIGIN      
        1 cgacctgcga gtgctggagc agcgcggggc ggcgccggac catccggtat cggccacgtg
       61 cctggaaacg gagtacctca agtgcctgat ttgcgaggtg gggtagggct ctggtggcac
      121 tgcttggtcg cggcggccaa gcagtggcac aagcaggcga tgacgtaaac cttggcagca
      181 caacgacttg cgacatccgc tcacaacaga cacccgccaa ctgggaactt tcctccaaag
      241 tcgtgcgtcc aaccggtgac aacgtggtgc aggacgactc ccgctggatc gaggccgctc
      301 gccggggcga tcccacggct tttggccaac tggtgcgcaa ataccaggac cggctggtga
      361 cggctctgct ctatgtttgc gggtcgcacg acgaagcgga ggacgtggcc caagaggctt
      421 tcgtccacgc ctacgttaag ctcgcaagtt tcgagggcgc gagtgccttt tacacctggc
      481 tgtatcgcat cgccatcaac gcggcgatca gccggcggag aaggcggcgc gaggaggagt
      541 cggtcgagga acaacgcgat cgcttgggcc gcgagccgca agatgggggc gaaggagcag
      601 aggagaggct attgcgcgag gagcgggcgg tgctcgtccg gcgggccttg ggccggctga
      661 gcgacgagca ccgcgcgatc ctcgtgctgc gggaagtcga tggctgcgac tatgagcaga
      721 tcgcagcgat cctcgacgtt cccgtcggca cggtccgcag ccggttgcat cgcgcccggc
      781 tgcacctgaa ggagcaattg gacgtggtct tggggaaggc ggagcagcga gaataggcgg
      841 acgcactccg cggaagtgat tgagagcctg acatgacgca tttttccgac gaattgctga
      901 gtgcctatct ggatggcgag ctgtccgccg aggagcgggc cgccgtcaag tcgcgcctgg
      961 ccgagagcga agccgaccgt cggttgctgg aagagctgca agcgctgcgc agcgagctgc
     1021 agtcgctgcc gcgggccgag ttgacgtctg agtttaccga ccgcgtcgtg cgggcggctg
     1081 cggagtctca agagcacatc aacttgcaag tcactccggc agctgctgat cctggggctg
     1141 gaaggacacg ccgtttttcc tggccgctgt ggacggcagc ggtcgcggcg gggctggcga
     1201 tctgtgcggt gctgatcgtg cggaactggc cgctcggcga tgccaacgtg gtcgtcggac
     1261 ccggcccgat tccacccacc gtgccgtcgc cggctgagct gctcatcgcg cagcttcgcc
     1321 aggcagcgcc cacgcaggac gaagcggttg tcattcgcct gcgcgtgccc aagaattcgc
     1381 cgctgggccc gtcgctcgat gtcgccttgg ccgaagcggg aatccgccag cggccggcct
     1441 tcgatctcac cagcggcgcg atcgagctcg gcgccgccta tcgcaagcaa gtgcagcaaa
     1501 agccggctaa tgatctggtg gctgtcagcg acgctctctt cttaaacgct ccgctcagcc
     1561 agattgaaga ggccctcgcc gcgattgccg gtgctgatga ccgagccttg cgcctggccg
     1621 ctgagacgcg tttggccttg gccgatttgc ccaagccgca agaaggtccc gaaggtgaac
     1681 cggggagtga aaatgcaggc gggccgttct cgcagcggct caatgccggg atgttccgcc
     1741 tcgaaaagcg cgaatctgcc gccgcctggc cgaccgacgt tgttcccgcc gccctcaatc
     1801 cgcagcagac cgtcaagctg ctgattctga tcgagcaggt ggattaggcc ggacggggtc
     1861 gggcgtcaat tgcggcactg gtcctccatg tgcagaacga cagccgcaat tgactcccga
     1921 cccctttatc gtcgctagcc gacaaacgcc cgcgagacca tccgcaccaa ccttgcgact
     1981 agctgcggct gccggccaac ctggcgagca attcccgcag tgaaccggtt gaaagccagt
     2041 cggtgcggca ggttgaacag gcgggaagcc ccgctcgtga accgcagcga acgccgattc
     2101 gccggccggc gacgccgctc gtaagccgtg agcagatccc tctcgccggc gagtgcaagt
     2161 tcggcgatcg cgcggccatc ggcgatcgac gcattggcgc cttgcccgcc ggcgggactg
     2221 accggatgag cggcatcgcc catgatcagc gcgcctgcgc cgccgtaccg cggcgcatgt
     2281 ccccacggcc ggcgcacgcg catgaagtcg tccggaaact tgcgctggcc gacgaccgac
     2341 ttcaggctcg gatccatctc gatgaattcc tcccacgcgg cctgcgcccg tggcacatca
     2401 tcgaagatgc gcggccgcac gggaacaatc cccacggctt gccgctgcgc cgtcggaagg
     2461 cagccggcgg ccatgatccc cgagcgcgcc gagcgatagt ttggccacac gcggccggcg
     2521 ttcggccgaa actctgccgg ccaatcgccc ccgaagcaga gcaggtcgag cggaaacaat
     2581 cttgtcttga ggtcgatgcc gcacgcctgc cgtacgagcg aatgtccgcc atcgtccccc
     2641 accgtccaat cggcgagcca ctcgcgctcc tggccggtcg ccacgtcgcg cgaccgcgca
     2701 ccgatgatcc ggtctttgtt tttgaggatc tccttgactt ccacgccgcg gtgcagctcg
     2761 aacgactcca gccgcatcaa gagctcgcgt gtcaggttag ggttcgtgga ccagggatgg
     2821 acctgtgccc gccggagcgc gtccggggaa atcagccact ggaactcgtg cccgtcgaac
     2881 agctcgacac ccgccagcgg cacgatcgct tcgtcccagc gagtccgcgg aatcaaggtg
     2941 cagagcaggt cgagcgtggt cggccacaga atctccggcc ggctccacgg cggcggcccg
     3001 gtgctgcgtt ccaggacgac gacccgcttg ccgccgcggc ccagcatttc ggccagcacc
     3061 agcccgccga tccccccgcc gacgatcaga aagtcggtcg ccatatagcc atctta
//
LOCUS       DASUGB010000126         6367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_988040, whole genome shotgun sequence.
ACCESSION   DASUGB010000126 DASUGB010000000
VERSION     DASUGB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988040"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..2322)
                     /gene="recG"
                     /locus_tag="VFV87_02460"
     CDS             complement(217..2322)
                     /gene="recG"
                     /locus_tag="VFV87_02460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5102644.1"
                     /translation="MSTAPQKTPAEQLATPVQFLKGVGPQRAELLAKLDLFYAADVLF
                     FFPRAYQDMSELREIDQLEEGKLASVVGVIEEVDLRNTSPGKNILGMLLKSGTKYLRC
                     IWFNQPWMRQKLPEGRRILVSGEPKLEGLRWEMAHPRVEYLADDEDVPAGRILPVYSL
                     TEGINQSQMRRIVAGVVESHAGLVEDTLPDDFLDAHQLWPIKAALPQIHSPNDQGSLK
                     QAQRRFVYQELLVLQLALAIRRWRLEHQRQGPPLPASAKIDSRITRLFPFELTAAQRQ
                     AIDEISRDMGRPFPMNRLLQGEVGSGKTVVAMYAMLLAVAHGYQAALMAPTEILARQH
                     LQTLSTALAASKVRLALLTGSLSPAERREALEKIAAGEVDVVIGTHAVTHAVAKSGVE
                     FAKLGLVVIDEQHKFGVRQRAALKRAGIDPHYLVMTATPIPRTVSMTLFGDLDVSTLH
                     DPPPGRQPVHSYLAGDEQRMRWWHFFRTKLDEGRQGFVIAPLVEDSESVEAANVQQTF
                     ETLSRGELASYRLGMLHGRMTPVEKDGAMQAFRSGRTHVLVATGVVEVGVDIPNATLM
                     TIEGGERFGLAQLHQLRGRISRGAHPGYLCVFATPQTDESQQRLDAFTKTTSGFELAE
                     LDFQLRGPGDLFGTKQHGLPPLRIADLKRDTAVVEEARAAAQQLIQSDPDLQHPAYSR
                     LRRMVFHRYGEALDLGDVG"
     gene            2489..2842
                     /locus_tag="VFV87_02465"
     CDS             2489..2842
                     /locus_tag="VFV87_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102645.1"
                     /translation="MRRLQFSLGMLFVLVTLSAVGVDFYRRRELVVTAYRRNLIRGAV
                     GEMRAAESLDSWDSRNGTPGHPTPSQRAKAAQWLAEAEYHQRLAKAYRRAAWFPWYSV
                     DEESHTCEFDDADKP"
     gene            complement(2846..3406)
                     /locus_tag="VFV87_02470"
     CDS             complement(2846..3406)
                     /locus_tag="VFV87_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5102646.1"
                     /translation="MSTAVLSTPKLLTAEEYLLLPDNVDRRELRRGVVVVMNPPGFRH
                     GEVCGNIHFYLASFVREHQLGRALTNDSGIITERDPDTVRGADISFYSYKRVPKGQSP
                     LGYAGASPEIVFEVVSPYNTRKDIASKTGEYLQAGINVVCVVDAQHGTVELHYPDQPS
                     ATLRGNDPLTFAELNGFSLPVRKLFE"
     gene            complement(3492..3752)
                     /locus_tag="VFV87_02475"
     CDS             complement(3492..3752)
                     /locus_tag="VFV87_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102647.1"
                     /translation="MHTVEMLERLTDLARQAGYTIRHEWLGGAGGGKCEFAGRKWIFV
                     DLSLSVVEQLDQVAAALKDDPAVWTLDLPVPITRALGRRMAA"
     gene            3910..4734
                     /locus_tag="VFV87_02480"
     CDS             3910..4734
                     /locus_tag="VFV87_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5102648.1"
                     /translation="MSITAEERDARSAAEFAARITAQPADRIAALEQQLAQAQKLTAL
                     GELLSTTTHEFNNVLMTILNYARMGLRHKDEATRDKALDKIHTAAQRAAKITASILAA
                     ARNRSGGFEPTDLKQIVADTLVLMEREFAKYRISVETQLADVPEVSAIGNQIQQVLLN
                     LLINARQAMTGGGRLLVRLEHDPAAGTVDLVVRDSGCGIPEEKLPRIFEPFFTTKTGP
                     DETGKGGTGLGLSACHKIIVAHGGRIRVESTVGIGTQFTIKLPVAGRAPASLARTP"
     gene            complement(4806..5558)
                     /locus_tag="VFV87_02485"
     CDS             complement(4806..5558)
                     /locus_tag="VFV87_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102649.1"
                     /translation="MVVVSSTGCVGGWFHRRGGDGSQAIENPLFVPPMDRECLWNNAV
                     DVVDNYFRIEREERVRLIGGVLTEGRIDTFPQSGSTILEPWRTDSTPGYEKWLATLQS
                     IRRRATVRVIPIEDGYLLEVIVQKELEDLDKPENATAGGATLRYDGSLIRQQGAPGRH
                     AVTLGWIPIGRDQSLEQRILAQISQRLDLNGVIVDHLPPVMDEFPAEELPAVPGEPQS
                     RELNRLELAPPENLPFSAPERLPAPLERLPLP"
     gene            complement(5662..>6367)
                     /locus_tag="VFV87_02490"
     CDS             complement(5662..>6367)
                     /locus_tag="VFV87_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102650.1"
                     /translation="EAWLMSRENAAVLYEQLKARGDFREHSTPSTEVQNGQSRKLTRT
                     QPRTYSRGVQIKSEFPFYDLIPGRIDEGYSLSISPLMSLDGKSVEAAIDCQIDQVEKL
                     VPVTIDLPIGQQNQRVAVQVPQVVSWRLNERFRWPADQVLLLSCGVIANPAGGGAGPL
                     APLLTPIVGASNRADALLFVEHRGAAGDPHAEGIARASAESPPTPAPVVSPIGSAPLK
                     DPAVKPASAISPFRRY"
BASE COUNT         1125 a         2001 c         2122 g         1119 t
ORIGIN      
        1 taggagaaag ttgcgcgatg gcagtggccg gtgaaaaatc tgggaaagtt gcgcgcaact
       61 ggatgttgcg cgggcggttc gcgaagcgga gcttcggaac gggcgttccc aagcaggagc
      121 ttgggaccga gagagaacga gggacatgcg agcccaaagc gtcagggagc gacaaagcgc
      181 gagactccct cgctgacgct tcgggttagt gcggcgctac cctacatccc ccaggtcgag
      241 ggcttcgccg tagcggtgga aaaccatccg gcggaggcgg gagtaagcgg gatgttggag
      301 gtcggggtcg gattgaatca gttgctgggc ggcggcgcgg gcttcttcga cgacggctgt
      361 gtcgcgcttg aggtcggcaa tgcgcagagg aggcaggcca tgttgcttgg tgccgaacag
      421 gtcgccagga ccgcggagct ggaaatcaag ctcggccagc tcgaagccgc tggtcgtttt
      481 ggtgaaggcg tccagccgct gttgcgattc gtcggtctgc ggcgtggcga agacgcacag
      541 gtagccggga tgagcgccgc ggctgatacg gccgcgaagc tggtggagct gggccaggcc
      601 gaaccgctcg cccccttcga tcgtcatcag cgtggcgttc ggaatatcga cgccgacttc
      661 gacgacgccc gtcgccacaa ggacgtgcgt tcgcccgctg cggaaggcct gcatcgcccc
      721 gtccttttcg accggcgtca tccgcccgtg cagcattccg aggcgatagc tcgccagctc
      781 gccgcggctg agcgtttcga aggtctgctg gacgttggcc gcctcgaccg attcgctgtc
      841 ctcgacgagc ggcgcgatga cgaagccctg gcggccttcg tccagcttgg tgcggaagaa
      901 atgccaccac cgcatgcgct gctcgtcgcc ggccagatac gaatggaccg gctgacggcc
      961 gggaggcgga tcgtggagcg tcgaaacgtc gagatcgccg aacagcgtca tcgagacggt
     1021 ccgcgggatt ggcgtcgcgg tcatcacgag gtaatgcggg tcgatgccgg cccttttgag
     1081 ggcggcacgc tggcggacgc cgaacttgtg ctgctcgtcg atgacgacca ggccgagctt
     1141 cgcgaactcg acccccgatt tggcaacggc gtgcgtcacc gcgtgcgtgc cgatgacgac
     1201 atcgacttcg cccgcggcga ttttttccag cgcttcgcgg cgctcggctg gcgagagcga
     1261 gccggtgagc aacgcgaggc ggactttgct cgccgcaagt gcggtcgaga gggtttgcag
     1321 gtgctgacgg gcgaggattt cggtcggggc catcagcgcg gcctggtagc cgtgggcgac
     1381 ggcgagcagc atcgcgtaca tcgccacgac ggtttttccg ctgccgactt cgccttgcag
     1441 caggcggttc atcgggaagg ggcggcccat atcgcgactg atttcgtcga tcgcctgccg
     1501 ctgagccgca gtcagctcga acggaaagag ccgcgtgatc cgcgaatcga tcttggcgct
     1561 ggccggcagt ggcggccctt gccgctggtg ctccagccgc cagcggcgga tcgccagggc
     1621 aagctgcagg acgagcagct cctgataaac gaacctccgc tgggcctgct tcaggctgcc
     1681 ctggtcgttg ggcgaatgga tttgcggcag ggcggctttg atcggccaga gctggtgggc
     1741 gtcgagaaaa tcgtcgggga gcgtgtcctc aacgaggccg gcgtgcgatt cgacgacgcc
     1801 cgccacaatc cgccgcatct gcgactggtt gatcccttcc gtgagcgaat agaccggcag
     1861 aatgcggccg gcgggcacgt cctcgtcgtc ggccaggtac tcgacgcgcg gatgggccat
     1921 ctcccaccgc aggccctcca gcttcggctc gccgctgacg agaatccgcc gcccctcggg
     1981 cagcttctgc cgcatccaag gctggttgaa ccagatgcaa cgcaggtatt tcgtgccgct
     2041 cttgagcagc atgccgagga tgttcttgcc ggggctcgtg ttccgcagat cgacttcctc
     2101 gataactccg acgacgctcg ccagcttgcc ctcttcgagc tggtcgatct cgcgcagctc
     2161 gctcatgtcc tgatacgcgc gcgggaagaa gaacagcacg tccgcggcat agaacagatc
     2221 gagcttcgcc aacaattccg cccgctgcgg gccgacgccc ttcaaaaact gcacaggcgt
     2281 agcgagctgc tcggcagggg tcttttgcgg cgcggtggac atgccgggat tctactggat
     2341 ggccgcgcaa gtcgctcgtc cgccttgtcc gcttgctttc gttgcgccct ttgcgagctt
     2401 ctgttgcttc cctccgcgcg gccgaggata ttgggaacag aaggacgcga aggacgcaaa
     2461 gaagatgaag cgattatggc caatgcgggt gcgtaggctt cagttcagcc tggggatgct
     2521 cttcgtgctg gtgacgttgt cggccgtcgg ggtcgatttc taccgtcggc gagagctggt
     2581 agtaacggcc tatcgccgca acctgatccg cggggccgtc ggcgaaatgc gtgccgcaga
     2641 atctctggat tcatgggact cgcgcaatgg aacccccgga catcccactc cgtctcagcg
     2701 agccaaggca gctcaatggc tggcggaagc tgaataccat cagcgactgg ccaaggcgta
     2761 tcgccgcgca gcctggttcc cgtggtactc ggttgacgaa gaatcgcaca cgtgcgaatt
     2821 cgacgacgcc gacaaaccat agcggtcact caaacagctt ccgcaccggc aggctgaagc
     2881 cgtttagctc ggcgaaggtg agcgggtcgt tgccgcgaag cgttgccgag ggctggtcgg
     2941 gatagtgcag ctcgacggtg ccgtgctggg catccacgac gcaaaccacg ttgatcccgg
     3001 cctgcagata ctcgccggtc ttgctcgcga tgtcctttcg cgtgttgtac ggtgagacga
     3061 cctcgaacac gatttcgggt gaggcgcccg cgtagccaag tggactctgt cccttcggta
     3121 ctcgcttgta gctgtagaag ctgatgtcgg ccccgcgaac ggtgtcggga tcgcgctcgg
     3181 tgatgatacc cgagtcgttg gtgagcgctc gcccaagttg gtgctcgcgg acaaagctcg
     3241 ccaaatagaa gtggatgttg ccgcaaactt cgccatgacg aaacccgggt ggattcatga
     3301 cgacgactac tccccggcgc agctcgcgcc gatcgacgtt gtcgggaagg agcagatatt
     3361 cctcagccgt tagcagtttt ggcgtggaaa ggacggcggt gctcatgatt gggtggcacg
     3421 gttagcgagt gcaagcaagc gtgctgattg gattatacac ccgggcgcgg ccgtcatcac
     3481 ctgagcgcga gtcacgccgc cattcgccgc cccagggcgc gcgtgatggg gacgggcagg
     3541 tcgagcgtcc agacggcggg gtcgtccttg agggcggcgg cgacctggtc gagctgctcg
     3601 acgaccgaga gcgagagatc aacgaaaatc cacttgcggc cggcgaactc gcatttgccg
     3661 ccgccggcgc cccccagcca ttcgtggcga atcgtatagc ccgcctgccg ggcgaggtcg
     3721 gtcagccgct cgagcatttc caccgtgtgc atcccagctt ctccagttca ccgcccttcc
     3781 atggcggggg cgggattata gctggcttgg ctgcagcgtc ccagttcgat tcgacgatac
     3841 ggactgatgg cggtaagtca ttgtccgcca gcggtttaag gcggaaactc tgggcaagac
     3901 acggcgaaca tgtcgatcac ggcagaagag cgagacgcgc gaagcgcggc ggagtttgcg
     3961 gcccgcatca ccgcgcagcc cgccgatcga atcgctgcgc tcgagcagca gctcgcacag
     4021 gcccagaagc tgacggcact gggtgaactg ctcagcacga cgacgcacga attcaacaac
     4081 gtgctgatga cgatcctcaa ctatgcccgc atgggcctgc ggcacaagga cgaagcgacg
     4141 cgcgacaagg ccctcgataa aattcacacc gccgcccagc gggcggccaa aatcaccgcc
     4201 agcatcctgg ccgccgcccg caaccgctcc ggcgggttcg agccaaccga tctgaagcag
     4261 atcgtggccg acacgctggt tctgatggag cgcgagttcg cgaagtaccg catctcggtc
     4321 gagacgcagc tcgccgacgt gccggaggtg agcgcaatcg gcaatcagat ccagcaggtg
     4381 ttgctgaacc tgctgatcaa cgcccggcag gcgatgaccg gcggcggccg gctgctggtg
     4441 cgcctggagc acgatccggc agcgggcacg gtcgatctgg tcgtgcgcga cagcggctgc
     4501 ggcatccccg aggagaagtt gccgcgcatc ttcgagccgt tcttcaccac gaagaccggc
     4561 ccggacgaaa cgggcaaagg gggcacgggc ctgggcctct ccgcttgtca caaaatcatc
     4621 gtggcccacg gcggccgcat ccgcgtcgaa agcaccgtcg gcatcggcac gcagttcacg
     4681 atcaagctac cggtggccgg ccgcgctccg gcgtcgctcg cgcgcacgcc gtaggacggg
     4741 tttccaaccc gtccttctgg gcaaccttct gtcacgatgc ggacgggtta gaaacccgtc
     4801 ctacgctacg gcagcggcaa gcgctccagc ggcgctggca ggcgctcggg ggcagagaac
     4861 ggcaagttct ccggcggcgc gagctcgaga cgattgagct cgcgggactg gggctcgccg
     4921 gggacggcgg gcagctcctc cgccgggaat tcgtccatga cgggcggcag gtgatcgacg
     4981 atgactccgt tcaggtcgag tcgctggctg atttgcgcga gaatccgctg ctcgagcgat
     5041 tggtcgcggc cgatcggaat ccagccaagg gtgacggcgt gccggcccgg cgcgccttgc
     5101 tggcggataa gcgagccgtc gtaccgcagc gtcgctcccc cggcagtcgc gttttcgggt
     5161 ttgtcgagat cttccagttc cttttgcacg atcacttcga ggaggtaacc atcctcgatg
     5221 gggatcacgc gcaccgtcgc tcgccgccga atcgattgca gcgtggcaag ccacttctca
     5281 tagccgggcg tcgagtccgt gcgccagggt tccaggatcg tcgagccgct ttgcgggaag
     5341 gtgtcgatgc gtccttcggt cagcacgccg ccgatcagcc gcacgcgctc ctcgcgctcg
     5401 atgcggaagt agttatcgac gacatcgacg gcgttgttcc acagacattc gcggtccatc
     5461 ggcggcacga agagcggatt ctcgatcgcc tgagagccat cgccaccgcg ccggtggaac
     5521 cagccgccca cgcagccggt cgagctaaca acaaccatca gcaagagcag caaaaaggtt
     5581 ggtgcgcaac gcgatccttt ccgccacgtc tcgcggctgt cttcccttgg caatttgcaa
     5641 tttgcactct gcattgtgca atcaatatcg tcgaaacgga gaaatggctg acgcgggttt
     5701 cacagccggg tccttgagcg gcgccgaacc gatcggactg accacaggcg ccggcgtcgg
     5761 tggggactcg gccgacgctc gagcgatgcc ctcggcatgc ggatcacccg ccgcaccgcg
     5821 atgctcgaca aacagcagcg catcggcccg gttactcgct ccgacgatcg gcgtcaggag
     5881 cggcgccagc ggccccgctc cgcctcccgc cggattggcg atcacgccgc acgacaggag
     5941 cagcacctga tccgccggcc agcgaaaccg ctcgttcagc cgccagctca cgacctgcgg
     6001 cacctgtacg gcgacacgct ggttctgctg gccgatcggc aagtcgatcg tcaccggcac
     6061 gagtttttcg acctggtcga tctggcagtc aatcgccgcc tcgaccgact tgccgtccag
     6121 cgacatcagc gggctgatcg agagcgagta gccctcgtcg atccgcccgg ggatcagatc
     6181 gtaaaacggg aattcgctct tgatctgcac gccgcgcgaa tacgtccgcg gctgcgtgcg
     6241 ggtgagcttt cgcgactggc cgttttgcac ttcggtgctg ggcgtgctgt gctcgcgaaa
     6301 gtcgccacgg gccttgagct gctcgtagag cacggcggca ttctcgcgcg acatcagcca
     6361 tgcctcg
//
LOCUS       DASUGB010000127         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1961149, whole genome shotgun sequence.
ACCESSION   DASUGB010000127 DASUGB010000000
VERSION     DASUGB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961149"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFV87_02495"
     CDS             complement(<1..1126)
                     /locus_tag="VFV87_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5102651.1"
                     /translation="MIRPLALALVFALVHLSRIAPSAGQEPDWKAGLARVKITPEQPV
                     FMAGYASRNKPYESIHDDLFAKVLVLEDAAGDKAVLVTADLIGFPAEIAGPIRARIAE
                     ATGILASRVLINASHTHTGPALSLDPTPGEGRALADSERTAAYTRHVQDQIAIAATKA
                     AAALVPARLAWGVGVVHFVMNRREFTERGVILGVNPRGLADRGVPVLRIDGADGKPLA
                     VVFGAACHNTTLGPQNYEISGDFAGHSQRLVEEKHAGCQAMFVQGCAGDANPYPRGSH
                     ELAGQHGEELAAEVCRVLETKLTPVKGPLRVAAGEVALPLAPPPSREELEKEAAAKSG
                     TRAWVAQQMLQRLDRSEKLPSEYVCPLAVWQFGGDLTLVAL"
     gene            complement(1160..1477)
                     /locus_tag="VFV87_02500"
     CDS             complement(1160..1477)
                     /locus_tag="VFV87_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5102652.1"
                     /translation="MKQIIAVVKPFVAEKVVEALRRAPLEALTVREVRGYNRQKRYLD
                     QYEQSEFALAFLPKVEIEAWVDDARVAEVVERITQIARTGRMGDGKVFVLPVARFERT
                     IEF"
     gene            complement(1496..1897)
                     /locus_tag="VFV87_02505"
     CDS             complement(1496..1897)
                     /locus_tag="VFV87_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5102653.1"
                     /translation="MPLVKFVKEKKEIEVPEGANLRSEALKAGINLNQGLNGMGASIN
                     RFMNCKGMGFCGTCRVLITKGMENTNKLTLREYLKFKTIVPTPVPDPVPALAYVGHED
                     TMRLACCTTVHGDIEVESGPEINLFGENFFS"
     gene            complement(2030..>2244)
                     /locus_tag="VFV87_02510"
     CDS             complement(2030..>2244)
                     /locus_tag="VFV87_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5102654.1"
                     /translation="SFRIRAMKPDERVYAEAARLANVSPAEILFTDDRADNVAGARAA
                     GWDAVLFESTNQLSGELRRRGIRTNY"
BASE COUNT          378 a          723 c          733 g          410 t
ORIGIN      
        1 ggagcgccac gagcgtcagg tcgccgccga attgccaaac cgccagcgga cacacgtact
       61 cgctgggcag cttctcactg cggtccagcc gctggagcat ttgctgggcg acccaagcgc
      121 gcgtgccgct cttggcagcg gcttcctttt cgagctcctc gcgcgagggc ggtggcgcga
      181 gcggcagcgc gacttcgccg gcggcaacgc gcagcggccc cttgaccggc gtcagcttcg
      241 tctccaggac gcggcagact tcggcggcca gctcctcgcc gtgctgaccg gccagttcgt
      301 gcgagccgcg cggatagggg ttggcgtcgc cggcgcagcc ctgcacgaac atcgcctggc
      361 agcctgcgtg cttctcctcc accagccgct gggagtggcc ggcaaagtcg ccgctgattt
      421 cgtagttctg cgggccgagc gtcgtgttgt ggcaggccgc gccgaagacg accgccagcg
      481 gcttgccatc cgcaccgtcg attcgcagca ccggcacgcc gcggtcggcc agcccgcggg
      541 gattgacgcc gagaatcacg cctcgctcgg tgaactcgcg ccggttcatg acaaagtgaa
      601 cgacgccaac gccccacgcg agcctggccg gcacaagggc cgcggctgcc ttcgttgcgg
      661 caatggcaat ctggtcctgc acgtggcggg tataggcggc ggtccgctcg ctgtcggcca
      721 gtgctcgccc ttcgcccggc gtgggatcga ggctgagcgc cggacccgtg tgcgtgtgcg
      781 aggcgttgat caagacccgg ctggcgagga taccggtcgc ctcggcgatt cgcgcgcgaa
      841 tcggacccgc aatctccgcc ggaaatccga tcagatcagc cgtgacgagc acggccttgt
      901 cgccggcagc gtcttcgagg accaggacct tcgcgaacag atcgtcgtgg atcgattcgt
      961 agggcttgtt gcgcgacgcg tagccggcca tgaagacggg ctgctcgggc gtgatcttga
     1021 cgcgcgccag gcctgccttc caatcgggct cctggccggc cgaaggcgcg atccggctga
     1081 gatggacgag cgcgaaaacc agcgccaggg caagcgggcg aatcatggtc ggctcccgag
     1141 aggctgtgat gcggggcggt cagaattcga ttgtccgctc aaaccgggcg accggcaaca
     1201 caaagacctt gccgtccccc atgcggccgg tgcgggcgat ctgtgtgatc cgctcgacga
     1261 cttccgccac gcgggcgtca tcgacccagg cttcgatctc gactttgggc aggaacgcga
     1321 gcgcgaactc cgactgctcg tactggtcga gatagcgctt ttgccggttg tagccccgga
     1381 cttcgcggac ggtgagggct tccagcgggg cgcgacgaag cgcctcgacg accttctcgg
     1441 ccacaaacgg cttgacgacg gcaatgatct gcttcatgac gccgccgcgg aaaccttacg
     1501 aaaagaagtt ctcgccgaac aagttgattt ccggacctga ttccacctca atgtccccgt
     1561 gcacggtggt gcagcacgcc agccgcatcg tatcttcgtg gccgacgtag gccagggctg
     1621 ggacggggtc cggaacgggc gtgggaacga tcgttttgaa tttcaggtac tcgcggagcg
     1681 tgagcttgtt ggtgttctcc atgcccttcg tgatcagcac gcggcaggtt ccgcaaaaac
     1741 ccattccctt acagttcatg aagcgattga tcgaggcacc cattccattg agcccttgat
     1801 tcaagttgat gccggcctta agcgcctcgc tgcgcaggtt ggccccttcg ggaacttcga
     1861 tctccttctt ttccttgaca aacttgacga ggggcatggc tggaaccaga tggaagggaa
     1921 atgtgtgaag ttgcaacctg ctggcaggta atggcttaga gcgaaccgtg ggccgcagcg
     1981 cctctctggc aatgttaccg catgattttg ccagcgctaa cggggcgatt cagtaattcg
     2041 tgcgaatgcc tcgccgccgc aattcgccgc tgagttgatt cgttgattcg aacaggacag
     2101 cgtcccagcc cgcggcccgc gcgccggcga cgttgtcggc ccggtcgtcg gtgaacagga
     2161 tctccgccgg cgagacattc gccagccggg cggcctctgc atacaccctt tcatccggct
     2221 tcatcgcgcg gatacggaag ctga
//
LOCUS       DASUGB010000128         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1822052, whole genome shotgun sequence.
ACCESSION   DASUGB010000128 DASUGB010000000
VERSION     DASUGB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822052"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..3258
                     /locus_tag="VFV87_02515"
     CDS             244..3258
                     /locus_tag="VFV87_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102655.1"
                     /translation="MLTRTTNYRPLHHHRQIPRLLGTPAVLAILATLTAGCGGGGGDV
                     DAPSTGAVSWSVVLGDHPTGSCFRPDTLVPLVVRASNAAGPISAPRYSITADPADGLA
                     TDSSGQVTVRGEGPLRLTVTYQGERAAGATLEPQVLDLMRDGTAPRITVTSPQRAAFR
                     VTASDDEAVSIQGSVVDDLSPVKAVYVNDAAMAVGGQSLSEPLVVQRPTHWGTNILQV
                     SAADACGNTASHAQSFLQSPHYLPAALARVPAARVSSASAMRLSAGVIDDLNRADFDD
                     FATITERALQKLLVPTLNSLLPQISPISQTGSISLAIGSGSCTVSADPQSGVSMATGS
                     PTLEIGLSQGALNVQVELRRLVIPVVYKAECTGPLGNVFFSETGTFTHSVDSIGGSVR
                     VTPRTLAGELELPSTLDLRLSGLSVDSSDRPALASIVNAAAPGLTAIIQGLLDTALED
                     AIASLTSSALESLFNSVTTMRTIQLAAPLAVDLNTGIEWHSINVTPQALTLVLDHYIY
                     PGSAGSPFASIGAINAGLPVRTLQGPPSTWMAAVSDDAANQVLWALWYGGALELQHLE
                     SYAATLPGLADVAQGLVLNLSASMPPVIERGTDVHRAVLGLGDVRVTGSVDLDSSPLF
                     SGSGMVSFDLFAGLRTDGRSGVDIERSQLNVRLDDFGQFYLHINSLQLDGHELADAVL
                     ADQLRDQFRRVVKRVLQGLLGGASAAMPLPPPTIGFPAALVGTNSSLLLDPLYLQRDT
                     DSFVVAIDPDDALHPVVQINNWSQLGAVALDAAREEARQWLEGVRLTCLISRNIASGP
                     AVWDTTYMPGDRVKAALLQAGAPESIARDWNRIFKSAWLHWGDDLYIPNLPWFPQFAA
                     DAISGDIRTIDATTGAVGTLTSVGIDGLSADRLRAALEQRLTDYLNQPGANAAIAAFA
                     GGFAERFNQQQQQGRIYDVHGTTVYPDFAVWGGVLVVGAIFAYMDPEDFVDLVADDFD
                     DAVGFLWPPPPVTGPRIGACQQAGRLDAVGFQ"
     gene            complement(3333..3425)
                     /locus_tag="VFV87_02520"
     tRNA            complement(3333..3425)
                     /locus_tag="VFV87_02520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3388..3390),aa:Ser,seq:cga)
     gene            3495..4244
                     /locus_tag="VFV87_02525"
     CDS             3495..4244
                     /locus_tag="VFV87_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5102656.1"
                     /translation="MTGAKRRKNLANRRGFMALGLAVLGFPRAAGAAGDTAQPAPAKP
                     PMQRARLPKRLAADFHSHIETRLPHLRSPFEVAARDTGISWRMLAALGYQESRWRPAA
                     VSNRGAQGVMMLMPETAKKMGVKNVFSPDENILAGARYLAYMRERIPKRIRDPDRTWL
                     AMAAYNIGIGHLEDARIITQMRKKNPDRWADVRVNLPRLSDPYWHNKVKRGYANGKET
                     AEFVERVSQFAAILENSVPDREAPRAVAKAR"
     gene            4388..>4642
                     /locus_tag="VFV87_02530"
     CDS             4388..>4642
                     /locus_tag="VFV87_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5102657.1"
                     /translation="MLQNPLPDHDVSLQGAPDGLSGLSRRSSLYGRSRETQALSMAFR
                     RAAAGGADLVVLSGPAGIGKTTLVRKMRAPLGQQSGYFISG"
BASE COUNT          806 a         1559 c         1478 g          799 t
ORIGIN      
        1 gaagccatcc tgcgttgcac gaatacgcgc gaaagcgagt tcctgcagcg gcagctcgac
       61 tcactcgcgc ccacgagctg atccaccggt acaggcggac tgccctaaca atatcaatcg
      121 ccaacaaccc ttccgtgcga cgggccatgg gccaaactac gcggcgacgc atgaaacatt
      181 gacacgacct gtcggagggg gccagtctgc ccgggcgcaa gcttcgcctg ataggaaccg
      241 gccatgctga caagaacaac gaactaccgg ccactgcatc accatcggca aattccccgc
      301 ttgctcggga cgccggccgt gttggcaata ttggcgacgc tcaccgcagg ttgcggtggc
      361 ggcgggggcg acgtcgatgc cccttcgaca ggggcggtca gctggtccgt ggtcttgggc
      421 gatcatccga ccggcagctg tttccgcccc gatacgctcg tgccgctggt ggtcagggcc
      481 agcaacgccg cgggcccgat cagcgcgccg agatattcca tcaccgccga tcccgcggac
      541 gggctggcca cggacagcag cggccaggtg acggtgcgag gtgaaggtcc gctgcgactc
      601 acagtcacgt atcagggaga acgtgccgcg ggagcaacgc ttgagcccca ggtcctggac
      661 ttgatgcgcg atggcaccgc tccgcggatc acggtgactt cgccacagcg cgctgcgttc
      721 cgcgtgacgg cgagcgacga cgaggccgtg agcatccagg gttccgtcgt cgacgatctg
      781 tcgcccgtga aggccgtgta cgtgaacgat gccgcgatgg cggtgggcgg ccagtcgctc
      841 agcgagccgc tggtcgtgca gcgaccgacc cactggggca ccaacatcct ccaggtgtcg
      901 gcggccgatg cctgcggcaa cacggccagt cacgcgcaga gtttcttgca gagccctcac
      961 taccttccag cggccctggc gcgagtgccg gccgcgcggg tatcgagcgc cagcgcgatg
     1021 cggctctccg cgggggtgat cgacgatctc aatcgggcgg acttcgatga cttcgccacc
     1081 atcaccgaac gcgcgctgca aaagctgttg gtgccgacgc tgaacagctt gctcccgcag
     1141 atatcaccga tcagtcaaac tggctcgata agcctggcca tcggatccgg cagctgcacc
     1201 gtatcggccg atccgcagtc cggcgtgagc atggccactg gctcgcccac gctggaaatc
     1261 ggcttgtcgc aaggcgcgct caacgtgcag gtggagctgc ggcgattggt gattcccgtc
     1321 gtatacaagg cggaatgcac tgggccgctc ggcaatgtct tcttcagcga gaccggcacg
     1381 tttacccact ccgtcgactc gatcggcggc agtgtccgcg tgacaccgcg gaccttggcc
     1441 ggcgagctcg aattgccaag caccctcgac ctgaggctgt cgggcctgtc cgtcgattcg
     1501 tcggaccggc cagccctggc cagcatcgtg aatgcggcgg cacccggact caccgcgatc
     1561 atccagggct tgctcgacac cgcgctcgaa gatgccatcg cctccctcac ttccagtgca
     1621 ctcgaatcgc tgttcaattc ggttaccacg atgcgaacga tccagctcgc agcaccgttg
     1681 gcagtcgacc tgaataccgg aatcgaatgg cactcgatca atgtcacgcc gcaggcgctg
     1741 acgctggtgc tggaccacta catctatccg ggttcggcgg gttcaccgtt tgcctcgatc
     1801 ggtgcgatca atgctggcct gcctgttcgc acactgcagg gtccgccaag cacgtggatg
     1861 gccgcggttt ccgacgacgc cgccaaccag gtgttgtggg cactctggta cggcggcgcg
     1921 ctggaactcc agcacctgga gagctacgcg gcgacgttgc ccgggctggc ggatgtcgcg
     1981 caggggctgg ttctgaatct gtcggccagc atgcccccgg tgatcgaacg cggcaccgat
     2041 gtccaccgcg ctgtactcgg actgggtgat gtccgcgtta cgggcagcgt cgatctggac
     2101 agcagcccgc tgttctccgg cagcggcatg gtgagcttcg atctatttgc cggcttgcgt
     2161 accgatggac gatcaggtgt cgacatcgag cgcagtcagc tcaacgtgcg gctcgatgat
     2221 ttcggacagt tctatctgca catcaacagc ctgcagctcg acggccacga gctggcggat
     2281 gcggtgctcg ccgaccagct gcgggaccag ttccgccgcg tggtcaagcg tgtgctgcaa
     2341 ggcttgcttg gcggcgccag cgcggccatg cctctgccgc cgccgacgat cggctttccg
     2401 gctgcgcttg tcggcacgaa ttcgagcctg ctgctcgacc cgctttattt gcagcgcgat
     2461 accgacagtt tcgtggttgc catcgatcct gacgatgccc tgcatccggt ggtgcagatc
     2521 aacaactggt cgcagctggg tgccgtggca ctcgacgcag cgcgcgaaga agcgcgccag
     2581 tggctcgaag gcgtgcggct cacgtgcttg atctcgcgca acatcgcgtc cgggccagcc
     2641 gtgtgggaca ccacgtacat gcccggtgac cgcgtgaaag cagcgctgct gcaggccggc
     2701 gcccccgaat caatcgcgcg cgactggaac cggattttca agagtgcgtg gctgcactgg
     2761 ggcgatgatc tctacatccc caacctgccc tggtttccgc agttcgcggc cgacgccatc
     2821 tccggagata tccgcaccat cgacgcgacg acgggcgccg tcggcacgct gacctccgtt
     2881 ggcatcgatg gcctttcggc ggacagactg cgtgccgcgc tggagcagag gttgacggat
     2941 tacctgaatc agcccggcgc gaacgctgca atagcggcat ttgccggcgg ctttgctgaa
     3001 cggttcaatc agcaacagca acagggccgc atctacgatg tgcacggaac cacggtctat
     3061 ccggattttg ccgtgtgggg tggcgtgttg gtcgtgggcg ccatcttcgc gtacatggat
     3121 cctgaagact tcgtggatct ggtggcggac gacttcgacg atgccgttgg ctttctctgg
     3181 ccgcctcctc cggtgaccgg accgcgcatt ggcgcctgcc agcaagcggg gcgtctcgac
     3241 gcggtgggat ttcagtaacc agtcggcaga cagccgttcg ttcctcgctt acgctcggga
     3301 cccattcatg cgcggcaaag cggaatgaga aatggcggag agggtgagat tcgaactcac
     3361 gtgccggaat tacccgacca tccgatttcg agtcggcgcc gttatgaccg cttcggtacc
     3421 tctccgcgcg agactttgac gggcaccttc gaaattaggg ccgtcccacg aggggcgcgt
     3481 attctagccg aacgatgacc ggagccaaga ggcgaaaaaa tctggccaat cgacggggct
     3541 tcatggccct gggccttgcc gtcctgggat tcccccgggc tgccggcgct gcgggggaca
     3601 ccgcccagcc cgcccccgcc aagccgccga tgcagcgcgc ccgcctgcca aaacgcctgg
     3661 ccgccgattt ccacagccac atcgagaccc ggctgccgca cctgcgctcg ccgttcgagg
     3721 tggccgcgcg cgacacgggg atttcctggc gcatgctcgc ggccctcggg taccaggaat
     3781 cgcgctggcg tccggccgcg gtgtccaacc gcggcgccca gggcgtgatg atgctgatgc
     3841 ccgaaaccgc caagaaaatg ggcgtcaaga acgtgttttc gcccgacgag aacatcctcg
     3901 cgggcgcccg ctatctcgcc tacatgcgcg agcgcatccc gaagcgcatc cgcgatccgg
     3961 atcgcacctg gctcgcgatg gcggcctaca acatcggcat cggacatctc gaggacgcgc
     4021 gcatcatcac ccagatgcgc aagaagaacc ccgaccgctg ggccgacgtg cgcgtcaacc
     4081 tgccgcgcct gtccgatccc tactggcaca acaaggtcaa gcgcggctac gcgaacggca
     4141 aggaaaccgc cgagttcgtg gaacgcgtct cgcagttcgc cgcgatcctg gagaactcgg
     4201 ttcccgaccg cgaagctccc cgcgcggtcg caaaagcgcg ctgaatccag cctggaagcc
     4261 gcgtccgcgg ccatcgattg ccacccttgt gacgcaggcc cgctacgcac ttccgcgcgg
     4321 ccttttcggg tcaagcggac agaccgggcg ccgataaatc ccaggcgcca tgaaccaccc
     4381 gactccgatg ctccagaacc cgctccccga ccacgacgtg agcctgcagg gcgcgccgga
     4441 cgggctctcc ggactcagcc ggcgctcgtc tctttacggg cgcagccgcg agacccaggc
     4501 actgtccatg gcgtttcgcc gtgcggcggc cggcggcgcc gacctcgtgg tgttgtcggg
     4561 gcccgcgggc atcggcaaga ccacgctggt gcgcaagatg cgcgcgccgc tcggccagca
     4621 atcgggttat ttcatcagcg gc
//
LOCUS       DASUGB010000129         4805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_849613, whole genome shotgun sequence.
ACCESSION   DASUGB010000129 DASUGB010000000
VERSION     DASUGB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849613"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..2826)
                     /locus_tag="VFV87_02535"
     CDS             complement(157..2826)
                     /locus_tag="VFV87_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102658.1"
                     /translation="MAQAFDAYQAWLGIPPHEQPPNHYRLLGLLLFESNPALIDSAAS
                     RATAQVRGFQSGPYKLVAQRVLGEIAGARQCLLEPGHKAAYDRELQARMQGGAAAPGP
                     ALAPQIVPPMPASSPRQMQALQPVVVQVPLPGQVPLPAAPLPASAPLPSGAMPVVVQV
                     PTHAASAATPIRPAPDSKQNESGPKKAPGFSLPQPVKIALGGAAGLFLGYFVVYFLTG
                     RDLLGILPSQKPPPANRTVAENPSPRPRENDRPRPNPRPSGSNSQPSGFSTPSGFSNP
                     PPQTFSPAPQPNPFAGPSESPSLPPTGQSDPPPVGPSNPSGIRPQIVIVTPQGTVVNP
                     VPPNSAPLPGTTAAPAPAPPPRPKLPAPSADEQKAMLAELESIYSAEFERGAKADGRK
                     EFVTFLLATARKLKTDPVAQFVLCRQAYDRAMRLEDFITAADVIDELERGFEVEGYRL
                     RMHLLTESARAARAPEERMPLVLFALDLADHAIATQRPEDVGKLTNTAEGLARNIPNR
                     DVKSHTTTRCIELRKAADELIPVATARAKLQSDPGDPAASLIDGKYRCFALGDWAGGI
                     KLLAQSEDPTLAAAAKLDLDTKADDRQAAVAIGDAWFDLAQADPGLAPAFARARHWYQ
                     QALSGADGLEKVKLDKRIEQISAMNLPAPQDASARPQAPTLATARFLLTRMKAFETTN
                     VISAITPQTVRTQGWYIYSDMLRVDNDATSARIQSPVNPTGEYQVALRVRRSTTTPHA
                     GMFVVGLPHVRSQFAVVIDYPVPGKGFASFITLGGLKKPDDNPTLKVTENLVPRLGTD
                     QQHIIICAVKLDEISVQLDGQELIQYRGDMSKLTMSKEWAVPDTRCMFLGSHQGGFYV
                     HGWVVSPLVADDGTPLPLLKDPLEARVGPPIRP"
     gene            3118..>4805
                     /locus_tag="VFV87_02540"
     CDS             3118..>4805
                     /locus_tag="VFV87_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5102659.1"
                     /translation="MSEAFDPYYLWLGIPPQEQPPNHYRLLGVRDFEDNSEVISYALD
                     QRCAHLRSFQAGKRGAESQKLLNEVAAAGVCLLDPAKRQQYDQELRARISRAGNHAPP
                     PAPPVAPATKKIPVARPLATAAAAVAPPPGAPSMSVVSSTTAATRTPPPSSLPMLAAI
                     GVAACVLLIGTATFAGWWIFARSGSSDPQPPLVANVPKPPAAKVNSPPTPKGGENPIS
                     KPREVEVTNSAPQNPSIPPPEPQPPDRPSPAPIPTPAPPNPTDTTVWLAEGENEFALR
                     QVNGTWVEFRPDEQWWYDGGVTALPDGIELYDRRRGVRVRLNNRMELKSARQDWAVVA
                     PGHWCPESELPEFARQSPVQPPPGLPVMLPATYALVFCGRETLQMPIPAEIRTPGADF
                     TVEMWVRFDPFQPGGALLSLGGGPTSLATHGLMNSAGATAVALRLSATGNRDLIVLQP
                     DQWRHVALVRGGPVLQLYVDGKPAHSESLPGNFSLPGSHCTIGSRTSGWHGMLRDCRL
                     SRGARYTAPFTPPLRFKNDEQTLALLDTTKNVNGSIVGDLSGHGHKAIGSNLHW"
BASE COUNT          827 a         1541 c         1614 g          823 t
ORIGIN      
        1 cgctgcacca gaaggcgcag ctcaagtacg tcgtctgtgc gaagtgcgga gagcgcgagc
       61 tggtgctcgt cgatggcctg cagaacgttt ccggcgcgga gtagccacag aaaatccgaa
      121 gcgtaagcga gggatcgggc aagagtgccg gccgcgctac ggccgaatcg gcgggccaac
      181 gcgggcttcc agcgggtcct tgagcagcgg cagcggcgtg ccgtcgtcag ccaccagcgg
      241 cgagacgacc cagccgtgga cgtaaaaccc gccctgatgc gagccgagga acatgcaccg
      301 cgtatcgggc accgcccact ccttgctcat cgtcagcttg ctcatgtcgc cgcgatactg
      361 aatcagctcc tgcccgtcga gctggactga aatctcatcc agcttgacgg cgcagatgat
      421 gatgtgctgc tgatccgtgc ccagacgcgg aaccaggttc tcggtcacct tgagcgtggg
      481 gttgtcgtcg ggctttttca gaccgccgag cgtgatgaag ctggcgaaac ccttccccgg
      541 cacgggatag tcgatgacca cggcaaattg cgagcggacg tgcggcaggc cgaccacaaa
      601 catgccggcg tggggcgtcg tggtagagcg gcggacgcgg agggccactt gatactcgcc
      661 cgtcggattg acgggcgact ggatccgtgc cgaagtcgca tcgttatcga cgcgcagcat
      721 gtcggaatag atgtaccagc cttgcgtgcg aaccgtttgc ggcgtaatcg cggagatcac
      781 gttcgtcgtt tcaaacgcct tcatgcgggt gagcaggaac cgcgcggtgg ccagcgtggg
      841 cgcttgtggc ctggccgagg cgtcctgcgg cgcgggaagg ttcatcgcac tgatttgctc
      901 gatccgcttg tcgagcttga ccttttccag gccatcggcg ccggacaggg cctgctgata
      961 ccagtgccga gcgcgggcga aggccggcgc caggccgggg tcggcttgcg cgagatcgaa
     1021 ccacgcatcg ccgatcgcca ccgcggcctg ccggtcatcg gctttggtat cgaggtcgag
     1081 cttcgcggca gcggcgagcg tggggtcctc ggattgggcc aggagcttga ttccgcccgc
     1141 ccaatcgccg agggcaaagc aacgatactt gccgtcgatc aggctggcgg ccggatcgcc
     1201 tggatcggac tgaagcttgg cgcgggcggt cgcgactgga atcagctcgt cagccgcctt
     1261 gcggagctcg atacagcggg tggtggtgtg cgacttgacg tcgcgattgg gaatgttccg
     1321 cgccaagcct tcagccgtat tcgtcagctt gcccacatcc tcgggccgct gtgtggcgat
     1381 ggcatgatcg gccaggtcca gtgcaaacag gaccagcggc atgcgctcct cgggcgctct
     1441 ggctgcccga gcggactcgg tgagcagatg catccgcaga cgataccctt ccacctcgaa
     1501 gccgcgttcc aactcgtcga tgacatcagc cgccgtgatg aagtcctcca gccgcatggc
     1561 gcggtcgtac gcctggcggc agaggacgaa ctgggcgacg ggatcggtct tcagcttccg
     1621 cgccgtggca agcagaaaag tgacgaactc cttgcgtccg tccgccttgg cgccgcgctc
     1681 gaattcggcg ctataaatcg attccagctc cgccagcatc gccttttgct catccgcaga
     1741 cggcgcgggc agtttgggcc gcggaggcgg cgccggagcc ggtgcggccg ttgttcccgg
     1801 caacggagca gaattcggcg gtaccggatt cacaacggtt ccctgcggcg tcacgatcac
     1861 gatctgcggg cggatgccgg agggattgct gggaccaacc ggtgggggat ccgattggcc
     1921 cgttggcggc agagaaggcg actcgctagg accggcgaat ggattgggct gcggggcagg
     1981 cgagaacgtt tggggcggcg ggttcgaaaa tcccgatggc gtgctaaagc ctgacggctg
     2041 ggagttggat ccgctcggcc gcggattcgg cctgggccgg tcattttcac gcggccgcgg
     2101 tgatggattt tcggccacag ttcgattcgc gggcggcggc ttctggctgg gaagaatacc
     2161 cagcaaatcg cggcccgtga ggaaatagac gacaaagtag cccaagaaaa ggccagccgc
     2221 acctcccagc gcgatcttca ccggctgggg caaggagaag ccgggggcct tcttggggcc
     2281 agattcgttc tgcttgctgt caggcgcggg acgtattggt gtggcagcac ttgcggcgtg
     2341 cgtcggaact tgcaccacga ccggcatcgc cccggatggc agcggcgctg aggcgggcag
     2401 tggcgccgcc ggcagcggaa cctggccggg caacggcacc tgcacgacga ccggctgcag
     2461 cgcttgcatc tgccgcggcg aactcgccgg catcggcggc acgatctgcg gcgcaagcgc
     2521 cggacctgga gcagccgccc cgccttgcat tcgagcctgc aactcccggt cataagccgc
     2581 cttgtgcccc ggctcgagca aacactgccg cgcgccggcg atttcgccca gcacgcgctg
     2641 ggccaccagc ttataggggc cgctctggaa tccgcgcacc tgcgccgtcg cccgactggc
     2701 cgcgctgtca atcagggccg gattgctctc aaacagcagc agccccagca ggcgatagtg
     2761 attcggcggc tgctcgtggg gaggaatgcc cagccacgcc tgatacgcat caaaggcttg
     2821 agccataacc ttcctcgccg caccgagatc aacgcctggt acggcgattg tatccataga
     2881 aagcggggtt cgccagcaaa gtgatgcccg gccaatgcgg gatgtgaaga ttcccgaccg
     2941 ggtggtctct cgtttgtaac gcgaagattg acgcgtcgcg gaaagtgggg ataggattga
     3001 ccttcgaaac aaggggcggg aaagccgcgg cgcggaccgg ctcgtcttat ctctgcctcg
     3061 caggccagga tcgacttacg cccgcaccga tcgccgcccg ttgcacggat ggagtccatg
     3121 tccgaagcgt ttgaccccta ttatctgtgg ctcgggattc cgccccagga acagccgccg
     3181 aatcactatc ggctgctggg ggtgcgggat tttgaggata attcggaggt gatttcgtac
     3241 gccctcgacc agcgctgtgc gcacttgcgg tcgtttcagg cggggaagcg cggggccgag
     3301 tcgcaaaagt tgctcaacga agtcgccgcg gccggcgtgt gcctgctcga tccggccaag
     3361 cggcagcagt atgaccagga attgcgggct cgcatttcgc gggcgggcaa tcatgctcca
     3421 cctcctgcgc cgccggttgc tcccgccacg aagaaaatcc cggttgccag gccgctggcg
     3481 actgcagccg cggcggtcgc tccgccgccg ggcgctccgt cgatgtcggt tgtgtcgtca
     3541 acaaccgcgg caaccagaac tccgccgccg tcctcgctcc cgatgctggc ggcgatcggc
     3601 gtggccgctt gcgtgctgct gattgggacc gcgacgtttg ccggctggtg gatcttcgcc
     3661 cgcagcggct cttcggaccc gcagccgccg cttgtggcga acgttcccaa gccgcccgcc
     3721 gcgaaggtca acagcccgcc aacgcccaag ggcggggaga atccaatttc caagccgcgg
     3781 gaagtcgagg tcacaaactc tgcgccgcaa aatccgtcga ttcctccccc tgagccccag
     3841 ccgccagatc gcccaagccc agcgccgatc ccgacgccag cgcctcccaa tcccactgac
     3901 acgaccgtct ggttggccga gggcgaaaac gaatttgcct tgcgccaagt caacggcacc
     3961 tgggttgagt tccggccgga cgagcagtgg tggtacgacg gcggggttac cgcgctgccg
     4021 gacggcatcg agctgtatga tcggcggcgc ggcgtacgcg tgcggttgaa caaccggatg
     4081 gagctgaaat cggcccgtca ggattgggca gtcgtcgccc ccggccattg gtgccccgaa
     4141 agcgagctgc ccgagtttgc ccgccaatcg cccgttcagc cgccgcccgg tttgccggtc
     4201 atgcttccgg cgacgtacgc actagtcttc tgcgggcgcg aaacgctgca aatgccgatc
     4261 ccggcggaga ttcgcacgcc gggcgccgat ttcacggttg aaatgtgggt ccgcttcgat
     4321 ccgttccagc cgggcggcgc actgctctcg ctgggcggcg gccctacgag cctggcgact
     4381 cacggcctga tgaattctgc aggggcgaca gccgtcgcac tgcgcctgtc cgcgaccggc
     4441 aaccgcgacc tgatcgtcct ccagcccgac cagtggcggc acgtggcgtt ggtacgcggt
     4501 ggcccggtcc tgcagctcta cgtcgatggc aagccggccc acagcgaaag ccttcccggc
     4561 aatttctcgt tgccggggtc gcactgcaca atcggcagcc gaacgagcgg ctggcacggc
     4621 atgctgcgcg attgtcgcct gtcgcgcgga gccaggtaca cggcgccgtt cacgccgccg
     4681 ctgcgcttca aaaacgatga gcagacgctg gccctgctgg acacgacgaa gaacgtcaac
     4741 ggctccatcg tcggagattt gtcggggcac ggccacaaag cgatcggttc caacctgcat
     4801 tggat
//
LOCUS       DASUGB010000130         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_849817, whole genome shotgun sequence.
ACCESSION   DASUGB010000130 DASUGB010000000
VERSION     DASUGB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849817"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFV87_02545"
     CDS             <1..761
                     /locus_tag="VFV87_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5102660.1"
                     /translation="YMRQPWGLSDIYLIRDPEYHGVLKIDPERLNSVVRTAVESGLQF
                     TAHSVGDGAVHTLLDAYEETNKTIPVRPTRPCITHCNFMSREAIDQMARLGVVADIQP
                     AWLYLDARTLSAQFGNDRLRYFQPLKSIFERQVIAGGGSDHMQKIGSLRSINPYNPFL
                     GMWVTITRQARWFEGQLHPEEALSREQAIRFYTMNNAHIVFLDDQIGSLEAGKLADLI
                     VLDRDLLKCPVDDIRETQVLASYLGGKQVHPRPR"
     gene            complement(762..1163)
                     /locus_tag="VFV87_02550"
     CDS             complement(762..1163)
                     /locus_tag="VFV87_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5102661.1"
                     /translation="MYVDDLPRAERFYVDVLELELVSRQEGRHVFFRCGDGMLLLFNP
                     LGSRESGSHDGGIDVPVHGSIGPGHIAFAACEDDLAAWAAHLEHHGVAIERWIDWPGG
                     GRSLYFRDPAGNSLELATPKIWGIPDDLPPG"
     gene            1338..2282
                     /locus_tag="VFV87_02555"
     CDS             1338..2282
                     /locus_tag="VFV87_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102662.1"
                     /translation="MAGRPFKRTTNGFTLIELLVVIAIIGILVALLLPAVQAAREAGR
                     RTSCSNNLKQIGLAIQGYHDIHGVFPPGGVNFGTCCSTESYTSWPISILPHLEQGGLS
                     GQYYHAFTNEAPENEFVRQQFLRTYACPSEPGTKALAKPESGPANDFNLQYMPGSYRG
                     VGGRSDGTGWWDNFPQYTALPRKWRGVFHVVDGRLQQESFAAVEDGTSHTLLVGEYGT
                     QTRSRRRTFWAYSYGSYNKSDAVPSSRTLVNDWDRCTAIGGLGGIQACNHGWGSFHPG
                     VVQFLYCDGNVRPVNVTTDMSVFVNAATIAGREPGLLP"
     gene            2279..2719
                     /locus_tag="VFV87_02560"
     CDS             2279..2719
                     /locus_tag="VFV87_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102663.1"
                     /translation="MRGLGAGFDRAAAAALLAPLVAVVLGCGQSADVVPVSGVVTLGG
                     RPLAGASVTFQPIQGSTDSHAGASGSAGRTDADGRYELRLIATGASGAIIGKHRVTIT
                     TAEASPDNDAKLASGERVPKEWRDGSTTFDVLAEGTTRADFAIP"
     gene            complement(2743..>3653)
                     /locus_tag="VFV87_02565"
     CDS             complement(2743..>3653)
                     /locus_tag="VFV87_02565"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX5102664.1"
                     /translation="AGAQYERLPELSQIEPLPPAEGEMIAPGQSYFDYDAWTAAGAGP
                     TNATGDWEWQILPEGIIYQAYLANPKESRLGTQVFEQTGDGQFLWDSTLGGHVGLLRY
                     GTCDGIWPQGWQFDVEGNTNVRLNPDNERDLDAADFRIGAPITYGYARHRLKFGYYHL
                     SSHIGDEFLIRNPGFTRLNFVRETLILGYAYFATDNLRLYAEAGWAFITDVCEPWELQ
                     FGFDYAPSRPTGIRGAPFVAFNTHLREELDFSGNFVVQAGWAWRGDRSTHLFRIGLHY
                     YNGASNLFEFYNTFEQQIGGGVWYDF"
BASE COUNT          682 a         1166 c         1140 g          665 t
ORIGIN      
        1 cctacatgcg ccagccgtgg ggtctttccg acatctacct catccgcgac cccgaatatc
       61 acggcgtgct gaagatcgac cccgagcggc tgaacagcgt cgtgcggacg gcggtcgaat
      121 cgggtctgca atttacggcc catagcgtag gggacggcgc cgtccacacg ctcctcgatg
      181 cctacgagga aacgaacaag acgattccgg tccgacccac gcgcccttgc atcacccact
      241 gcaatttcat gagccgcgag gcgatcgatc agatggcccg tctgggagtc gtggccgata
      301 tccagcccgc ctggctgtat ctcgacgccc gcacgcttag cgctcaattc ggcaacgatc
      361 gcctccgcta cttccagccc ctgaagtcga tcttcgagcg ccaggtgatc gccggcggcg
      421 gctccgatca catgcagaaa atcggctcac tgcggagcat caacccctac aacccgttcc
      481 tgggaatgtg ggtcaccatc acccgccagg cgcgctggtt cgaagggcag cttcatcccg
      541 aagaggccct cagccgcgag caggcgatcc gcttctacac gatgaacaac gcccacatcg
      601 tgtttctcga cgaccagatc ggttcgctgg aagcgggcaa attggccgat ctaatcgtcc
      661 tcgaccgcga tttgctcaaa tgcccggtcg atgacatccg cgagacgcag gtgctggcct
      721 cgtacctcgg tggaaaacag gtccatccgc ggccgcgatg actaccccgg tggcaagtca
      781 tccgggattc cccaaatctt cggcgttgcc aattccaggc tgttgccggc agggtcgcga
      841 aaatagaggc tccgtccccc gcccggccag tcgatccacc gctcgatggc tacgccgtga
      901 tgctccaaat gggcggccca ggcggctaag tcgtcctcgc acgctgcaaa ggcgatgtgc
      961 cccggaccga tgctgccgtg gacgggaacg tcgatgccgc cgtcgtgcga gcccgattcg
     1021 cggctcccca ggggattgaa cagcagcagc atgccatcgc cacagcgaaa aaagacgtgg
     1081 cggccctcct gccggctcac caattcaagc tccagcacgt ccacataaaa gcgctcggcc
     1141 cgcggcaggt cgtccacgta caaacaggtc tcaagtactt ttgtaacttt catgcatttt
     1201 aagactgcag ggggcacggg gcgccaaggc gagaaccggg tcgctgattg ttgagaaccc
     1261 gctcccccgt tatggtagtg ggagacgtgc ggtcgcgcga actagtgcgc tacaacagtc
     1321 aaattgagga ggaggggatg gcggggcgtc ccttcaagag gacgacgaac gggttcacgc
     1381 tcattgaact gctcgtcgtc attgcgatca ttggcatctt ggtggcgttg ttgctgccgg
     1441 cggtgcaggc tgcgcgcgaa gctggtcgcc gcacctcgtg cagcaacaat ctcaagcaga
     1501 ttggtctggc gatccagggt tatcatgata tccacggcgt cttcccgccg ggcggcgtca
     1561 atttcggcac gtgctgctcg acggaaagtt atacctcctg gccgatttcg atcttgccgc
     1621 atttggagca aggcggttta tccggtcagt actaccatgc gttcaccaac gaagctcccg
     1681 aaaatgaatt cgttcgccag cagttcctgc ggacctatgc ttgcccctcc gagcccggga
     1741 ccaaagcgct ggcgaaaccg gaatcagggc ctgcaaacga tttcaacctg cagtacatgc
     1801 ccggctcgta ccgtggcgta ggcggacgca gcgacggcac cggctggtgg gacaactttc
     1861 ctcagtacac agccctcccg cgcaaatggc ggggcgtgtt tcacgtggtc gatggccgcc
     1921 tgcagcagga gtcgttcgcc gccgtcgagg acggcacatc gcacacgctg ctcgtcggag
     1981 agtatggcac acaaacacgt tcccgccgcc gcaccttctg ggcctactcc tacgggtcgt
     2041 acaataagtc cgatgcagtg ccctccagcc gcacattagt taacgactgg gaccgctgta
     2101 cggcgatcgg cggactcggc ggaattcagg cctgcaatca cggctggggg agctttcatc
     2161 cgggagtcgt gcaattcctg tattgcgatg gcaacgtgcg gcccgtgaat gtgacgacgg
     2221 atatgtcggt cttcgtcaat gctgcgacga tcgccggtcg cgagccaggc ctgctgccgt
     2281 gaggggcctg ggcgccggct ttgaccgggc ggccgccgca gcgctgctcg caccgcttgt
     2341 agccgtggtg ctgggctgtg gtcagtcggc cgatgtggtg ccggtttcgg gcgtcgtcac
     2401 gcttggcggc cggccgctgg ccggggccag cgtgacgttc caaccgatcc agggcagcac
     2461 cgattcacat gccggtgcga gcggatcggc cggccgcacg gatgccgatg gccgctacga
     2521 actgcggctg atcgcaacag gcgcttccgg tgcgatcatc gggaaacacc gcgtgacaat
     2581 caccaccgcc gaagcgtcgc ccgacaacga cgccaagctc gccagcggcg aacgtgtgcc
     2641 caaggagtgg cgcgatggct ccacgacgtt cgacgtactc gccgagggaa cgactcgcgc
     2701 tgactttgca attccctagc gccgcacgcc gcgaccgctt gctcagaagt cgtaccacac
     2761 gccgccgccg atttgctgct cgaacgtgtt gtagaactcg aacagattgc tcgcgccgtt
     2821 gtagtagtgc aggccgatgc ggaacaggtg cgtgctgcgg tcgccgcgcc aggcccagcc
     2881 ggcctgcacg acaaagttgc cgctgaagtc gagttcctcc cgcaggtgcg tgttgaaagc
     2941 cacgaagggc gccccgcgaa tgcccgtcgg ccgcgaaggg gcatagtcaa agccgaactg
     3001 aagctcccac ggctcgcaga catcggtgat gaacgcccag cccgcttcgg cgtacaggcg
     3061 caggttatcg gtggcgaaat aggcgtagcc cagaatcagc gtttcccgga cgaaattgag
     3121 ccgcgtaaag ccagggttgc ggatcaggaa ttcgtcgccg atatgcgagc tgaggtggta
     3181 gtagccgaac ttgaggcggt gccgggcata gccgtacgtg atcggagcgc cgatgcggaa
     3241 gtcggctgcg tcgagatcgc gttcgttgtc ggggttcagg cggacgttgg tgtttccttc
     3301 gacatcgaac tgccagccct gcggccagat gccgtcgcaa gtgccgtacc gcaacaggcc
     3361 cacgtgtccg ccgagcgtcg aatcccagag gaactgaccg tcgccggtct gctcaaagac
     3421 ctgcgtgccg agccgcgatt ccttcgggtt cgccagatag gcctgataga tgatgccttc
     3481 gggcaggatc tgccattccc agtcgcccgt ggcattggtc gggccggcgc cggccgcggt
     3541 ccaggcgtcg taatcgaaat agctctggcc gggggcgatc atttccccct cagccggcgg
     3601 cagcggctcg atttgcgaca gctccggcag gcgctcgtat tgcgcgccgg cct
//
LOCUS       DASUGB010000131         4035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_154737, whole genome shotgun sequence.
ACCESSION   DASUGB010000131 DASUGB010000000
VERSION     DASUGB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154737"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFV87_02570"
     CDS             complement(<1..443)
                     /locus_tag="VFV87_02570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5102665.1"
                     /translation="MNLRFAMAGLSLLIVAGCDTSAPSPEVSGENSGPTAPAVAHGPK
                     LPLEQVNLVVGDEKKFAELIAAHKGSVVLIDYWATWCGPCVEGFPHTVELSQKHKEPG
                     LATIAVSFDLLEDEQKVREFLADQGASFENVLSKYDGVNQEAAEG"
     gene            complement(553..1128)
                     /locus_tag="VFV87_02575"
     CDS             complement(553..1128)
                     /locus_tag="VFV87_02575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5102666.1"
                     /translation="MIRCTFVALALVALCAPVFAAELEIGAKAPDFKATGIDGKEYNL
                     ASVKDAKAVVVCFTCNVCPVAVAYEDRFVEFQKKYAAKGVKFIAINANKANPAEGIEA
                     MKTRAEEKGFNFPYAFDASGESASEYGARVTPHLFLLDGDRTVAYVGAFDDNQNNPKK
                     HYLTDAVDAVLAGKKVETTNTKAVGCGIRNK"
     gene            1444..2235
                     /locus_tag="VFV87_02580"
     CDS             1444..2235
                     /locus_tag="VFV87_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102667.1"
                     /translation="MPIEFQCPSCHARIRTPDQTAGKSARCPKCSTVITIPGAPGSMS
                     LPAPPGHVPKPAPQSAPNPLGFSAPNPLGAPAGNPLGSSSAHPLGGPPPGSMPNPFGD
                     KPAPGFAPPPNPYAPPSAAGYYQAQRPMYGSRDAVRSWLLGPAICCTIFAVVGLGFMA
                     MVVLGMVIDPDAIADQAPPDEAGKAGFYGFFVVYFAGGLITRLLQIAGAICMFRVRGY
                     SLAMTAAVCALLPCDLYCCALNLPIGIWALIVLNKPEVKAAFQQP"
     gene            2346..2672
                     /locus_tag="VFV87_02585"
     CDS             2346..2672
                     /locus_tag="VFV87_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102668.1"
                     /translation="MIQAKSYVRENGQGGLRIGELDVSLDSVVLAYQQGYSAEMIQQL
                     YPALMLEEVYGAIAFYLANQEEVHRYLQRQDSRWQQLRQSAAAQPSAVVARLRALAQS
                     GSQATP"
     gene            2669..3025
                     /locus_tag="VFV87_02590"
     CDS             2669..3025
                     /locus_tag="VFV87_02590"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5102669.1"
                     /translation="MSRPRFLADNDLNDAIVLGVARRDPAIEFIRLRDVGLERLDDLE
                     VLRFAARGNWMVISHDVNSMTGAAFALLAANEPMHGLLLTHQTDPVAPVIDSVILIWS
                     ASEAEEWIGLVEFLPL"
     gene            complement(3038..>4035)
                     /locus_tag="VFV87_02595"
     CDS             complement(3038..>4035)
                     /locus_tag="VFV87_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102670.1"
                     /translation="TGFLIGSLSTANEKDEFRRLFRDDQELHQRCEERIKLSTASGAD
                     GKPTCSLVAKAFMSAAIELADRIGKTPDYPSPLLFYAAPSKYQARYAEELLREGKLGR
                     ASLAWKEAQILFEELAQREMPLNDNWRGKLADLPRWRAKERGLWMQFDELCPGLRQRL
                     FEESLRNQPTELQQAMRAEADEWAPDWEDVEQALTTKDRAALALAREAAECGQTVEDI
                     ERCRIIVNLDQWLTRCQIEQTPESLRARDLLDRARAARESGHLESDNPENPGAQQLYR
                     SAFRSFASILREHPDCFENDLYCEDIVAEVRAARKLFGDDIIPAELARLRLRWAL"
BASE COUNT          725 a         1263 c         1225 g          822 t
ORIGIN      
        1 aagccctcgg cggcttcctg gttcacgccg tcatacttgc tgagcacgtt ctcgaagctg
       61 gccccctgat cggccaggaa ctcgcggacc ttttgctcgt cctccaacag gtcaaagctg
      121 acggcaatcg tcgccagccc cggctcttta tgcttctggc tcagctcgac cgtgtgcgga
      181 aagccttcga cgcacggccc gcaccacgtc gcccaataat cgatcaaaac gacactgcct
      241 ttgtgggcgg cgatcagctc ggcaaatttc ttctcgtcgc cgacaaccag attgacttgc
      301 tccagcggca gtttggggcc gtgggcgacg gctggcgcgg tcggcccaga attctcaccg
      361 gacacctccg gcgacggagc gctggtatcg cagccggcga cgatcaacaa cgagagcccc
      421 gccatggcga atcgcagatt cacgacaatg tcctcaacga acggagaatt caggcgcaca
      481 ggccaacaaa aaaccgccgc cccggaattg ggttccggtg cggcggacat ggttacgaat
      541 gatcaagttc cactacttgt tgcgaatgcc gcagccgacg gccttcgtgt tggtcgtttc
      601 gaccttcttg ccggcgagca ccgcatccac ggcgtcggtc agatagtgct tcttggggtt
      661 gttctggttg tcgtcgaagg cgccgacata ggcgacggtc cggtcgccgt cgagcaggaa
      721 caggtgcggc gtgacgcggg cgccgtactc actggccgat tcgcccgagg cgtcaaaggc
      781 atacgggaaa ttgaagccct tctcttcggc gcgggtcttc atcgcttcga tcccttcggc
      841 cgggttggcc ttgttggcgt tgatcgcgat gaacttcacc cccttggccg catacttctt
      901 ctgaaactcg acgaagcggt cttcataggc caccgcgacc gggcacacgt tgcaagtgaa
      961 gcagacgacg acggctttgg cgtccttgac cgacgccagg ttgtattcct tcccgtcgat
     1021 gccggtagcc ttgaaatcgg gcgccttggc gccaatttcc agctccgcgg caaagaccgg
     1081 ggcgcacagg gcgaccagcg ccaaagcgac gaacgtgcaa cgaatcatga gcatactcct
     1141 gagagagaaa cggaatgctg ggaaacgggt cagaaataac gcaagcggcc ggcgtgaagc
     1201 cagcgacgaa cttccggttg tacgcggcaa cggagggaag gttcgggcgg ctaattgttg
     1261 gccgcgccgg taggttgggt tgattgtaag ggctggagac cggatgtcaa ctttccgcag
     1321 aacctggcgg gggctattct accgtccgat ccgcttgcgg gatttgctag ctcgccgttc
     1381 tcagatgctt gggagccggc acttattgcc tagatggcgc caattccatc cgcgttacaa
     1441 tccatgccca tcgaattcca atgcccttcc tgccatgcga ggattcgcac tcccgaccag
     1501 accgccggga agtcggcccg ctgcccaaag tgcagcacgg tcatcacgat tcctggcgcg
     1561 ccggggtcca tgtcgctgcc ggccccgcct gggcatgtgc caaaacctgc gccacagtca
     1621 gcgccaaacc cgctcggctt cagcgcgccc aatccgcttg gggctccagc cggaaacccg
     1681 ctcggttctt ccagcgccca tccgctgggc gggccgccgc caggcagcat gcccaatccg
     1741 ttcggcgata agccggcgcc tgggtttgct ccgccgccga atccctacgc gccgccgagc
     1801 gccgcaggtt actaccaggc tcagcggccg atgtacggct cgcgcgacgc cgtccgctcc
     1861 tggctactcg ggccggccat ctgctgcacg atttttgccg ttgtcggatt gggcttcatg
     1921 gcgatggtcg tgctgggaat ggtcatcgac cccgatgcca ttgccgacca ggctccgccc
     1981 gatgaggcgg gcaaggcggg cttctacggc ttcttcgtcg tctatttcgc gggcggcctg
     2041 atcacgcgac tgctgcagat cgcgggcgcg atttgcatgt tccgtgtgcg cggctattcg
     2101 ctggcgatga cggccgcggt ctgcgcgctg ttgccctgcg acctctattg ctgtgcgctc
     2161 aacctcccca tcgggatctg ggcgctgatc gtcctcaaca agcccgaagt gaaagccgcg
     2221 tttcagcagc cgtaggtcag gttgcgcgaa gcaggccaag cgcttacggt tcccatcacc
     2281 gtcgcgcggc ggcggattgg gcaatacagc ctttacgtcg taagataatg ttgaagggga
     2341 tggacatgat tcaagccaag tcctacgtcc gcgagaacgg tcaaggcggg ttgcgaatcg
     2401 gcgagttgga tgtgtcgctg gattccgttg tcctcgccta tcagcaaggc tactccgccg
     2461 aaatgattca gcagctctac ccggccttga tgctggaaga ggtgtacggc gccatcgcgt
     2521 tctatctcgc caatcaggaa gaagtgcatc gctacctgca gcgccaggac agtcgctggc
     2581 agcagctacg gcaatcggcg gcggcccagc ccagcgccgt cgtcgcccgc ctgcgggcgc
     2641 tcgcccaatc gggcagccag gcgacgccat gagccggccg cgatttctgg ctgacaacga
     2701 tctgaatgat gctatcgtgc tcggcgtcgc gcgacgcgat ccggccatcg aatttattcg
     2761 actgcgggac gtgggacttg agcgcctgga cgatctggaa gttctgcgct tcgcggcgcg
     2821 cgggaactgg atggtgatct ctcacgacgt gaattccatg acgggcgcgg cgtttgccct
     2881 cctggcggcc aacgagccga tgcacggatt gctgctgacc caccagaccg atccggtcgc
     2941 acccgtcatc gacagcgtga ttctgatttg gtccgccagc gaggccgagg agtggatcgg
     3001 gctggtcgaa ttcttgccgc tgtgaggcag tacatagtta aagtgcccag cgaagcctga
     3061 ggcgagcgag ctcagcgggg ataatgtcgt cgccgaaaag cttgcgagcg gctctaacct
     3121 cggcaacgat gtcttcgcaa tacaagtcgt tctcgaagca gtcgggatgc tcgcgcagaa
     3181 tactggcaaa tgatcggaag gcactccggt acagttgctg tgcccccgga ttctccggat
     3241 tgtcactctc gagatgccct gattctcgag cggcgcgagc ccgatcaaga agatcgcgag
     3301 cgcgcaagga ttccggtgtt tgctctatct gacaccgcgt gagccactga tcgagattaa
     3361 cgatgatccg gcagcgttct atatcttcaa ccgtctgtcc gcactctgcc gcctcgcgag
     3421 cgagggcgag tgcggcccta tctttcgtgg ttagtgcttg ctcgacgtct tcccaatcag
     3481 gggcccattc gtccgcctcg gcgcgcatcg cttgttgaag ctctgtcggc tgattccgga
     3541 gactctcttc gaataggcgc tgccggagtc ccggacataa ttcgtcgaac tgcatccaga
     3601 gtcctctctc ttttgcgcgc cagcgcggca ggtccgccag cttccctcgc cagttgtcgt
     3661 tcagcggcat ttcccgttga gccagctcct cgaacaggat ttgcgcctcc ttccaggcaa
     3721 gagaggcgcg acccagcttt ccttcacgca gcaattcctc ggcgtatcgc gcttggtact
     3781 tgctcggagc ggcgtagaag agaagcggac tggggtaatc cggcgtcttt ccgatccgat
     3841 cggcaagctc gatggccgcc gacatgaacg ctttggcgac gagtgagcaa gtcggcttgc
     3901 cgtcggctcc gctggcggtg gacagcttta tgcgttcctc gcagcgctgg tgcaattcct
     3961 gatcgtctct gaacaggcgg cggaactcgt ccttctcatt ggccgttgac agcgaaccga
     4021 tcaagaaacc cgtct
//
LOCUS       DASUGB010000132         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_15875,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000132 DASUGB010000000
VERSION     DASUGB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15875"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFV87_02600"
     CDS             <1..343
                     /locus_tag="VFV87_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate transporter subunit"
                     /protein_id="HEX5102671.1"
                     /translation="KQAGAGRFEIVVPSVSILAQPPVALVDKFAARHGTSQVAQAYLE
                     HLYSPQGQQLAAKHYYRPAIGESVSAAERARFAELELFTIDQVFGGWKQAQHEHFDDG
                     GVFDQIYQPGK"
     gene            351..1190
                     /gene="cysT"
                     /locus_tag="VFV87_02605"
     CDS             351..1190
                     /gene="cysT"
                     /locus_tag="VFV87_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX5102672.1"
                     /translation="MRLAFRRHSVLPGFGLTLGYTLLYLSLIVLIPLSALLLRTSAMP
                     WQRFWAAVSDERVVAAYRLTFLAALVAAAINALFGFLVAWCLVRYRFPGRKLLDALVD
                     LPLALPTAVSGIALTAIFAKTGWLGQYLEPLGIKTAFSPLGVTIALTFVGLPLVVRTL
                     EPAFEELDADSEEAAASLGANRWQTFWRVIVPTMLPALVTGFALSLARGLGEYGSVVF
                     ISGNMPLRTEIASLLIVSKLEQYDYAGASAIAAVMLGASLAILLGINVLQWWVSRKYL
                     GRA"
     gene            1193..2200
                     /gene="cysW"
                     /locus_tag="VFV87_02610"
     CDS             1193..2200
                     /gene="cysW"
                     /locus_tag="VFV87_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX5102673.1"
                     /translation="MSLLLPLPASPRPAARDPLWLRIVLIVISAGFLGVLVALPLAVI
                     FIEALRGGLRAYFASFRDPAAWQAIKLTVLAAAIAVPANLIFGLAAAWAIAKFEFPGK
                     SLLITLIDLPMAVSPVISGMVYVLLFGLHGWLGPWLDRHNVQIIFAVPGIVLATIFVT
                     FPFVARELIPLMQQQGSEEEQAALSLGAGGWQTFWRVTLPNIRWGLLYGVILCNARAL
                     GEFGAVSVVSGHIRGKTNTVPLHIEILYSEYNAVAAFSMASLLALLALATLAAKTWLE
                     GKIAAADCDSAASAPIAATIGCDLEPAATFGRDLEPAATSGRDSAICATDGRGSVLEV
                     R"
BASE COUNT          329 a          801 c          737 g          447 t
ORIGIN      
        1 gaagcaggcc ggcgcgggcc ggtttgagat cgtcgtgccc agcgtcagca tcctggccca
       61 gcccccggtg gctctcgtcg ataagttcgc cgcccggcac ggcacgagcc aggtcgccca
      121 ggcgtacctc gagcacctct attcgcccca aggccagcag ctggccgcca agcattatta
      181 ccgccccgcg atcggcgagt cggtctcggc ggccgagcgc gcccggttcg cggaactcga
      241 gctgttcacc atcgaccagg tcttcggcgg ctggaaacag gcccagcacg agcatttcga
      301 cgacggcggc gtgttcgacc aaatctacca acccggaaag tgaggccgcc gtgcgactgg
      361 cttttcgacg gcacagcgtc ctgcccggct ttgggctgac gctgggctac acgctcctgt
      421 acctgagcct gatcgtcctc attccgcttt cggccctgct cctgcgtacc tccgccatgc
      481 catggcagcg gttttgggcc gcggtgagcg acgagcgcgt cgtggccgcg taccggctta
      541 cattcctggc cgcgctcgtc gcagcggcga tcaacgccct cttcggcttc ctcgtcgcct
      601 ggtgcctcgt gcgctaccgc tttcccggcc gcaagctgct cgacgcgctg gtcgatctgc
      661 cgctggcgtt gccgacggcc gtttcgggca tcgccctgac cgccattttc gccaagaccg
      721 gctggctggg gcagtatctc gagcccctgg ggatcaagac cgcgttttcc ccgctgggag
      781 tgacgatcgc cctgacgttc gtcggcctgc cgctggtcgt ccgcacgctc gagccggcct
      841 tcgaggagct cgacgccgac tcggaagaag ccgccgccag cctgggggcc aatcgctggc
      901 aaaccttctg gcgcgtcatc gtgccgacga tgttgccggc gctcgtcacc ggctttgccc
      961 tctcgcttgc gcgaggcctg ggcgaatacg ggtcggtcgt gttcatctcg ggcaacatgc
     1021 cgctgcggac ggagatcgcc tcgctgctca tcgtctcgaa gctcgaacaa tacgattacg
     1081 ccggcgcaag cgcgatcgcc gccgtgatgc tcggggcgtc actggccatc ctgctgggga
     1141 tcaacgtcct gcagtggtgg gtcagccgca agtatttggg gagggcctag gcatgtccct
     1201 cctgcttccc ctgcccgcca gcccacggcc ggcggcgcgc gacccgctgt ggctgcggat
     1261 cgtgctcatc gtcatttcgg ccggcttcct gggcgtgctc gtggcgctgc cgctggcggt
     1321 gatttttatc gaagcgctgc gcggcggcct gcgagcctat ttcgccagct ttcgcgaccc
     1381 ggccgcctgg caggcgatca agctgacggt tctggcagcg gcgatcgccg tccccgcgaa
     1441 cctgatcttc ggcctggccg cggcctgggc catcgccaaa ttcgaattcc ccggcaagag
     1501 cctcctcatc acgctgatcg acctgccgat ggccgtttcc cccgtgattt ccggcatggt
     1561 ttacgtcctc ttgttcggcc tgcacggctg gctgggcccg tggctggacc ggcacaacgt
     1621 gcagatcatc ttcgccgtgc cgggcatcgt gctggccacg atctttgtca cgtttccgtt
     1681 cgtcgcccgt gaactgatcc cgctgatgca gcaacaaggc tcggaggaag agcaggccgc
     1741 cctctcgctg ggggcgggcg ggtggcagac gttctggcga gtgacgctgc ccaacatcag
     1801 gtggggcctc ctgtacggcg tgatcctgtg caacgcccgc gcgctggggg agttcggcgc
     1861 cgtgtcggtc gtctcgggac acattcgggg caaaacgaac accgtgccgc tgcatatcga
     1921 aattctctac agcgagtaca acgccgtcgc cgccttcagc atggcctcgc tgctggccct
     1981 gctggcgctg gcgacgctgg ccgccaaaac ctggctggaa gggaaaatcg cagccgcaga
     2041 ttgcgattcc gccgcatctg cccccatagc cgcgaccatt ggttgcgatt tggaacctgc
     2101 cgcgaccttt ggtcgcgatt tggaacctgc cgcgaccagt ggtcgcgatt ccgcgatctg
     2161 cgcgaccgat ggtcgcggct ctgtattgga ggttcgatga gcatcacgat cgagcacgtc
     2221 acaaaaacct tcggcggtca cacggccctc gacgacgtaa gcctgaccgt caacgacggc
     2281 gagctggtcg ccctgctggg cccctcgggc tcgg
//
LOCUS       DASUGB010000133         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1961569, whole genome shotgun sequence.
ACCESSION   DASUGB010000133 DASUGB010000000
VERSION     DASUGB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961569"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFV87_02615"
     CDS             complement(<1..458)
                     /locus_tag="VFV87_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX5102674.1"
                     /translation="MRLCVFSASLLLVTLAARRPATADDLFPDKNLEAVVRTQVFEKR
                     NKPDPLVEADVVNISGIKYEKSLKLAGKITNLAGLEKCKSLASIELPHQEIVDLAPLK
                     DLKQLQSVILNDNKIQSVAPLAELTGIQLLWIQDNQISDVSPLAKIAAMGS"
     gene            729..2360
                     /locus_tag="VFV87_02620"
     CDS             729..2360
                     /locus_tag="VFV87_02620"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX5102675.1"
                     /translation="MLIGYVSDERYVALPGVEIELLGPRGSLAVRSRASGSLYADIEP
                     GRYEIVLAKEGYGFKRVALNLPTPQPHQFRMLKDDLLGYAWPKWVKSGERAEFRVHSD
                     AAYKLELWRYGWKKELVQPIGWFDEHGPRATVQITPDGDYTQTGVTWNKFGYTSPHHK
                     QYVTAPERSGLYYFHATNEAGRFFSFPWIVAPCQPKASVAVLASNITWNAYNNFGGRS
                     NYIHPDKLPPTPTANARLELNRYTNPEHVNYGTTDYAPLSFDRPEPVCHVPEHVQPTD
                     PIAGRAACHLAPAEWRFLAWLEREGFAYDYYAETQLHFGQLDLDAYHVLITTTHPEYW
                     SADMYFKVKEWVFQRGGRLMYLGGNGLNCDVEFVDEQTCIYRNDDERRLRPAWHSVGN
                     AIPGVPLSKFESRFHLRQESEASLLGILYDDRAIMTAAPYRVIDASHWVFEGTGLEDG
                     SIFGRKSLHERIPGGASGHETDKISPSSPKNVKLLSKGMNPGDGGAEMAIHEPSGGGA
                     VFSVGSITWPASILVDDAVSRITSNVLRRFSGAGF"
BASE COUNT          490 a          731 c          765 g          453 t
ORIGIN      
        1 gaacccatcg cggcgatctt ggccagcggc gaaacgtcgg aaatctggtt gtcctgaatc
       61 cagaggagtt gaatgcccgt caattcggcc agcggggcga cgctctgaat cttattgtcg
      121 ttcaaaatca ccgactggag ctgcttgaga tctttcagcg gcgccagatc gacgatttcc
      181 tgatgtggca gctcgatgct cgccaggctc ttgcacttct ccagccccgc gaggttggta
      241 atcttgcccg cgagcttcag gctcttttcg tatttaatgc ccgagatatt cacgacatcc
      301 gcctcgacca gcggatcggg cttgttccgc ttctcaaaca cctgcgtccg caccaccgct
      361 tccaggttct tgtccggaaa gaggtcgtcc gccgtcgccg gccgacgggc agccagcgtc
      421 acgaggagca gggacgctga gaaaacgcaa agcctcatgg gaaacaccat gccgatcaaa
      481 ggtggagagg gactaagcag gcgagtgcaa ccaacgcggg ccaaaccaag gcggggtgac
      541 cgagtatcag aatacttgga ccctccaggc gattccagca cccaatcaac acgttgggaa
      601 ttgatagcgt cattcaactc caacggcatc tctcgcaagg actcgggtct gatccaaacg
      661 agtcgcatcc agcgagtata gtagtcaccc agtgaatcga gcacagaggc agccgaaagg
      721 agaaccaaat gctcattggc tatgtcagcg acgagcgcta tgtcgccctg ccgggcgtgg
      781 agatcgaatt gctgggcccg cgcgggtcgc ttgctgtgcg gtcgcgggcg agcggatcgc
      841 tctacgccga tatcgagccg ggccgctatg agattgtgct ggccaaagag ggctacggct
      901 tcaagcgggt cgcgctcaac ctgccgacgc cgcaaccgca ccagttccgg atgctcaagg
      961 acgatttgct gggctatgcc tggccgaagt gggtcaagag cggcgagcgg gcggaattcc
     1021 gcgtgcatag cgacgcggcg tacaagctcg agctgtggcg ctatggctgg aaaaaggagc
     1081 tggtgcagcc gatcggctgg ttcgacgagc acggcccgcg ggcgacggtg cagatcacgc
     1141 ccgacggcga ttacacgcag accggcgtga cctggaacaa gttcggctac accagccctc
     1201 accacaagca gtacgtgacc gcgcccgagc ggtcggggct gtattacttt catgcgacga
     1261 acgaagcggg ccggtttttc tcgtttccgt ggatcgtcgc gccgtgccag cccaaggcga
     1321 gcgtcgccgt cctcgcatcg aacatcacgt ggaacgccta caacaacttc ggcggccgca
     1381 gcaactacat ccatcccgat aagctgccgc cgacgccgac cgccaacgcg cgtctggagc
     1441 tgaaccgcta caccaacccc gagcatgtga actatggcac gaccgattat gctccgctgt
     1501 cgttcgaccg gccggagccc gtctgccacg tgcccgagca tgtgcagccg accgatccga
     1561 tcgcgggacg ggccgcttgc cacctggcgc cggcggaatg gcggtttctg gcgtggctgg
     1621 agcgggaagg cttcgcgtac gactactatg cggagacgca gcttcacttt ggccagctcg
     1681 acctggacgc gtaccacgtg ctgatcacga cgacgcatcc ggagtactgg tcggcggaca
     1741 tgtatttcaa agtcaaggag tgggttttcc agcgcggggg gcggctgatg tatttgggag
     1801 ggaatgggct caattgcgat gtggaattcg tggatgaaca gacctgcatt taccgcaacg
     1861 acgacgagcg ccgcctgcgg ccggcgtggc actcggttgg gaacgccatc cctggcgttc
     1921 cgctctcgaa attcgaaagc cgctttcatt tgcgtcagga atcggaggcg tcgctgctgg
     1981 gcatcctgta cgacgaccga gcgatcatga cggccgcgcc gtaccgcgtg atcgacgcca
     2041 gtcactgggt cttcgaaggt accggtctgg aagacggcag tatttttggc cggaaatcgc
     2101 tgcacgagcg gattcccggc ggcgcttcgg gtcacgagac cgataagatt tcgcccagct
     2161 cgcccaaaaa cgtcaagctg ctctccaagg ggatgaaccc cggcgatggc ggcgcggaga
     2221 tggcgattca cgagcctagc ggcggcggtg cggtgttctc cgtcggctcg atcacctggc
     2281 ctgcctcaat cctggtcgat gacgccgtca gccggatcac gtcgaatgtc ctgcggaggt
     2341 tcagtggggc aggcttttag cctgccatgt ggaaggggtc gggagtcaat tgcggccgac
     2401 acctttccat aagcaaagtc ggtgaccgca attgactcc
//
LOCUS       DASUGB010000134         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_710202, whole genome shotgun sequence.
ACCESSION   DASUGB010000134 DASUGB010000000
VERSION     DASUGB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710202"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFV87_02625"
     CDS             <1..490
                     /locus_tag="VFV87_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102676.1"
                     /translation="PRPRISAVSDSKGEFQILRHPAPMFLHAKTSNGLLAGLVKIKDD
                     AKEAVIPIGPLAAAKGRLVDGQFDLPVPGRQIRFGFRIEMESGGWTTQFGGTVTTDAR
                     GEFMLDGLIPGHSFEVDVVVEEGPDGRPHGWRTVATVTAAKAEVVELGDVGLRLAESK
                     DK"
     gene            616..974
                     /gene="ssrA"
                     /locus_tag="VFV87_02630"
     tmRNA           616..974
                     /gene="ssrA"
                     /locus_tag="VFV87_02630"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1269..>2365)
                     /locus_tag="VFV87_02635"
     CDS             complement(1269..>2365)
                     /locus_tag="VFV87_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102677.1"
                     /translation="KFEKRGQSGLEISEIFAKTAEHADSLAVIRSMFAHVPNHEPSLM
                     LMNCGDSVQPRPSVGAWVLYGLGTENQNLPGFIAMVPGGMPIKDSENWQSAFLPGAYQ
                     GTFIDSKHEQPGKLIENITHPHATTAMQRRQLDLLSKLNAEHRAGRLDDRLDARIQSF
                     ELAFRMQTEAAEAFDLSRETQETRDLYGSGVHGRQTLIARRLLERGLRYVQLWHGAGQ
                     PWDNHDNIEANHKKLAAEIDQPIAALLTDLKRLGMFDDTLVIWGGEFGRTPSVELGGD
                     GKSKLGRDHNHYGFSVWLAGGGIKGGTAYGATDEFGFKAAENPTSVHDLHATILHLLG
                     FDHERLTYRYAGRDFRLTDVSGHAIREILA"
BASE COUNT          419 a          723 c          752 g          471 t
ORIGIN      
        1 cccgcggccc cgcatctccg ctgtgtcaga cagcaaagga gaattccaga ttcttcggca
       61 cccggcgcct atgttcctac atgccaagac gagcaacggc ctattggcgg gcctcgtgaa
      121 gatcaaagac gacgcgaagg aagcggttat tcccatcggt cccttagccg cggctaaggg
      181 gcggctggtg gatggccaat tcgacctgcc ggtgccagga cgtcagattc gatttgggtt
      241 tcgcattgaa atggagagcg gtggttggac cacgcaattc ggtggcacgg taacaacaga
      301 tgctcgcggc gaattcatgc tggatggact cattccgggg cattcgttcg aggtggatgt
      361 cgttgtagag gaagggccag atggccgacc ccatggttgg cgcacggtag ccacggtaac
      421 cgccgcaaaa gccgaggtcg tagaactggg cgatgtcggg ctgcgcctgg cggaatcgaa
      481 agataagtag gccgtcgttc gcgtctatcg gcctgcttcc attctcccgc tctctagccc
      541 ccttcagcca cgcgtctata attgccgatg aaggaagagg gaaacctctc ccggccgctg
      601 agttgctgag acattggggg cgaactggat tcgaccggat gctcggaagc gaaggtggcg
      661 tgccgtggtt ggtcagttgg ccacgttaaa agctgaccac aatgttactt gccgagagca
      721 atctcgccct ggctgcctaa ctagcgtagc cactggctgc gggccttcgc cggagaggcc
      781 caaaagccag tcgccaattc cggaccgccg cgagacacgt cggataccct cgcggttaaa
      841 cttcgtttcc gactagttgc tctggcagtg tgccggtgga cggccagacg gcgaatgcaa
      901 agcaccagct acgcacgtag aagccttcgt ggacagatcg cgggacgcgg gttcaattcc
      961 cgccgcctcc acttagccag ctgcgacggc cagtcacagg cagggttgga gcacatcaaa
     1021 ccgctacgct gaaaggagtt gtggcaacgt ggcgttccgt gcttgccgat tggccctcaa
     1081 ggaaagtcgt tgggggtgat gacaatcttg ttttcccgca gtttgcacga cagcacctgc
     1141 cagcactcga acaaatcggc cgtttcgtgc gtatcgcggc aaactcgaaa ataagtgcgc
     1201 cggccctgca gtcgcaggtg gcttatttct tggcaggtct gtgatgggtg ggattgcccg
     1261 cgcaagcact aggccaggat ctcgcggatc gcgtgtccgc tgacgtcggt caggcggaaa
     1321 tcgcggccag cgtagcggta ggtcagccgc tcgtggtcga agccgagcag gtgcaggatg
     1381 gtggcgtgca ggtcgtgcac gctggtcgga ttctcggcgg ctttgaagcc gaattcgtcg
     1441 gtcgcgccgt aggccgtgcc ccccttgatg ccgccgccag ccagccagac cgagaagccg
     1501 tagtggttgt ggtcgcggcc gagcttcgat ttgccgtcgc cgcccagctc gaccgaaggc
     1561 gtgcggccga attcgcctcc ccagatcacc agtgtgtcgt cgaacatccc cagccgcttc
     1621 aggtcggtca acagcgccgc gatcggctgg tcgatttccg cggcgagctt cttgtggttg
     1681 gcctcgatgt tgtcgtgatt gtcccacggc tggccggcgc cgtgccaaag ctgcacgtag
     1741 cggaggcccc gctccagcag ccgccgggcg atcagcgtct gccgcccgtg cacgccactg
     1801 ccatacaggt cgcgcgtctc ttgcgtctcg cgcgacaggt cgaacgcctc ggccgcttcg
     1861 gtctgcatgc ggaacgccag ctcgaacgac tgaattcgcg cgtccagccg gtcgtcaagc
     1921 cgccccgcgc ggtgctcggc gttcagcttg ctcagcaaat cgagttgccg tcgctgcatg
     1981 gcggtcgttg cgtgcgggtg cgtgatgttt tcgatcagct tgcccggctg ctcgtgtttg
     2041 ctgtcgataa acgtcccctg ataggccccc ggcaggaacg ccgactgcca gttttctgaa
     2101 tctttgatcg gcatcccgcc gggcaccatt gcgatgaagc ccggcaggtt ctggttctcg
     2161 gtccccaggc catacagcac ccaggccccg acgctcggcc gcggctgcac cgaatcgccg
     2221 cagttcatca gcatcagcga gggctcatgg ttgggcacgt gggcaaacat cgagcggatc
     2281 accgccaagc tgtcggcgtg ctcggcggtc ttcgcgaaga tctcgctgat ctccaggccg
     2341 ctttggccgc gcttctcgaa cttga
//
LOCUS       DASUGB010000135         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_154916, whole genome shotgun sequence.
ACCESSION   DASUGB010000135 DASUGB010000000
VERSION     DASUGB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154916"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..646)
                     /locus_tag="VFV87_02640"
     CDS             complement(29..646)
                     /locus_tag="VFV87_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102678.1"
                     /translation="MGLYAKYVFAPLMDWSLRRQDIEALRGRVVSQAAGDVLEIGFGT
                     GLNLPHYTADVRRLTLLDSAELLPRRVARRVETCRAGSIERLITSAERLPLEDQSLDC
                     VVSTWTLCSIRDVAPALDEIRRVLRPGGKLLLMEHGRSDDPNNARWQDRLNPLQRLVA
                     CGCNLNRPIDRLLENAGFGLERLSRERMENVPQVLAEIYVGAAAP"
     gene            complement(747..1367)
                     /locus_tag="VFV87_02645"
     CDS             complement(747..1367)
                     /locus_tag="VFV87_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5102679.1"
                     /translation="MSEDLTARIRTGDTHALAEFIEAHRPQLMAFIERQLGTALRRKV
                     EPDDIYQETSAEAVRALPEAELGDRDPFSWLCQIAERRIIDLHRRFFEAQKRDAGREV
                     PLGAGGSGTESQPAGLINMLVASMTTPSQAFSRNARELKLQEALLKLPGDQQEALRMR
                     YVENLPSKQIADKLGKSDAAVRVMLTRSLKKLQELLADDISRTSLH"
     gene            1474..>3594
                     /locus_tag="VFV87_02650"
     CDS             1474..>3594
                     /locus_tag="VFV87_02650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5102680.1"
                     /translation="MTSSLTPENATQAWDELARRLEEFMEAWGAAPEPPLADFLPEAP
                     PVQRRMVLIELIKIDLEHRTDRGLSKQLESYTSEHPELLENGEPPCELIYEEYHIRRS
                     AGLTVTPQHYYDRFPKSAGALKRLMGTEGLSQTTQLVPAKRITGIVPGQRLDDFDLLV
                     ELGKGAFASVFLARQISMGRLVALKVSADKGNEPRMLAAFDHPNIVRVFDQRQLPDRQ
                     VRLLFMQFAPGGTLADVVREVRATPPSGRSGKMLLTAVDAALAKSGTIGPDDSTTRRR
                     IADATWPETVCRLGVQLAQALDHAHKKDTLHRDVKPANVLLSAEGSPKLADFNISFSS
                     QIEGVTAAAYFGGSLAYMSPEQLEACNPNHDRRPDELDGRSDLFSLAVVLWELLHGER
                     PFADEAPDGGWSQMLAGMAHRRRDSVALPKTKPGDPVEARLDQVLRKALSPDPAQRHA
                     DGAALARDLLLCLNPRAWDLVHNMTSGWRDFARREPMALLVPAVLPPFIFAGLFNLSY
                     NLNHFIQDLINDPKLNYPTGLALVAAFWFLVIPINAVLYPLGIGLVWWFAAPLARCVR
                     KVAGGGSADPEELQRARARAISLGHVVAAFGLGLWVVAGLAFPIGMNIMVGVGRFDIY
                     AHFFLAMFISGVISCCLPFLATTWLSIRVFFPALLAGSAPNSAEHRRLQALSRYAGWY
                     MWLSPVGPLGAIFLIVISGGETQRQ"
BASE COUNT          590 a         1207 c         1141 g          656 t
ORIGIN      
        1 gtccactgcc tctcgcggag cgagaggcct acggtgctgc cgcgccgacg tagatctccg
       61 ccagcacttg cgggacattc tccatccgct cacggctgag tcgctccagg ccaaagccgg
      121 cgttttccag cagccgatcg atcggccggt tcagattgca cccgcaggcg accaggcgct
      181 gcagcggatt gagccggtcc tgccagcggg cgttgttcgg atcgtcgctg cggccgtgtt
      241 ccatgaggag cagcttgccg ccgggccgca gcacgcggcg gatttcatcc agcgcggggg
      301 ctacatcgcg aatcgagcag agcgtccacg tgctcacgac gcaatcgagc gactggtcct
      361 ccagcggcag ccgctcggca gatgttatca gccgctcgat actgcccgct cggcaggtct
      421 ccacgcgccg ggcgactcgc cgcggcaaga gctccgcgct atcgagcagc gtcagccggc
      481 ggacatccgc cgtgtagtgc ggcaggttca gcccggtccc gaagccgatc tccagcacgt
      541 cgcccgcggc ctgcgagacg acgcgcccgc gcagggcctc gatgtcctgc cgccgcagcg
      601 accaatccat cagcggagcg aagacgtatt tggcgtagag gcccactggc agggtccaaa
      661 gtccaaagtc caacgtccaa tgtccaatgt ccaatgttca aagtcagaac atgacaacct
      721 tggactttgg accctggact ttggactcag tgaaggctcg tccgcgaaat atcgtcggcc
      781 aagagctcct gcagcttctt cagcgagcgg gtgagcatga cgcggacggc ggcgtcactc
      841 ttgccgagct tgtcggcgat ctgcttgctg ggcaggttct cgacgtaccg catccgcagg
      901 gcctcttgct ggtcgcccgg cagcttgagc agggcttcct gcagcttcag ttcgcgggcg
      961 ttccgcgaaa acgcctgcga tggcgtcgtc atgctagcaa cgagcatgtt gatcagccct
     1021 gccggctgcg actcggtccc gctgccgccg gcgcccagcg gtacttcgcg gccggcatcg
     1081 cgcttttggg cctcgaaaaa ccgccggtgc aggtcaatga tccgccgctc ggcgatctgg
     1141 cagagccacg agaacgggtc gcggtcgccc agctccgctt cgggcaaggc ccgcaccgcc
     1201 tcggcgctcg tctcctgata gatgtcgtcc ggctccacct tccgccgcaa tgccgtccca
     1261 agctgccgct caataaaggc cataagctgc gggcggtggg cctcgatgaa ttcggccagg
     1321 gcgtgggtgt cgccggtgcg gatgcgggcc gtcaaatctt cgctcatcgg aatcctcggg
     1381 ccatcgtgaa tcaggaacct gacggtagtg tagccaattg gcggtgcgtc cactacaacg
     1441 acagagtagc gccggaatcc tggcagccat cacatgacct cttccctcac gcctgaaaac
     1501 gccacgcaag cctgggacga gctcgccagg cggctggagg agttcatgga ggcctggggc
     1561 gccgccccgg agccgccgct ggccgatttc ttgccggaag ctccccccgt gcagcggcgg
     1621 atggtgctga tcgagctgat caagatcgac ctggagcacc gcaccgaccg cggcctgtcc
     1681 aagcagctcg aaagttacac gtcggagcac cccgagctgc ttgagaacgg cgagccgccc
     1741 tgcgagctga tctacgagga gtatcacatc cgccgcagcg ccggactgac ggttacgccg
     1801 cagcactatt acgaccgctt ccccaaatcg gctggggccc tcaagcggct catgggaacc
     1861 gaggggctct cgcagacgac gcagctcgtg cccgccaagc ggatcacggg catcgtaccc
     1921 ggccagcgtc tggacgattt cgacctgctg gtggaactgg gcaaaggagc attcgccagt
     1981 gtgtttctgg cccggcagat ctccatgggc cggctcgtcg cgctcaaagt ctccgccgac
     2041 aagggaaacg agccgcggat gctggccgcc ttcgaccacc ccaacattgt ccgcgttttc
     2101 gaccagcgcc aattgcccga caggcaagtg cggctcttgt tcatgcagtt cgcgccgggg
     2161 gggacgctcg ccgacgtggt ccgcgaggtc cgggccacgc cgccgtccgg ccgcagcgga
     2221 aaaatgctgc tcaccgcggt cgatgccgcg ctggccaaga gcggcacgat cggtccggac
     2281 gattccacga ctcgccgccg aatcgcggac gccacctggc cggaaacggt gtgccgcctg
     2341 ggcgtgcaac tcgcccaggc cctcgaccac gcccataaga aggacacgct gcaccgcgac
     2401 gtgaagccgg ccaacgtgct cctgtccgcc gaaggctcac ccaagctggc cgacttcaac
     2461 atcagcttct cgtcgcagat cgaaggcgtg accgccgcag cttatttcgg cggcagcctg
     2521 gcctatatgt cgcccgagca gctcgaggct tgcaatccga atcacgatcg ccggccggat
     2581 gagctcgatg ggcggagcga cctgttcagc ctggcggttg tgctctggga gctgttgcac
     2641 ggcgagcggc cgtttgccga cgaggccccc gacggcgggt ggtcgcaaat gttggctggc
     2701 atggcccacc gccgccgcga ctccgttgct ctgccgaaga ccaagccggg ggatccggtc
     2761 gaggcccggc tggatcaggt cctgcgcaag gccctctcgc ccgatcccgc ccagcgtcac
     2821 gccgacggcg ccgccctggc ccgtgacctg ctcttgtgcc tcaacccgcg ggcctgggac
     2881 ctcgtccaca acatgaccag cggctggcga gattttgccc gccgcgagcc gatggccttg
     2941 ctggtgcctg cggtgctgcc gccgttcatc tttgcggggc tgtttaacct ctcgtacaac
     3001 ctcaaccact tcattcagga tctcatcaac gatccaaagt tgaactatcc gacgggcttg
     3061 gcgctggtgg cagccttttg gtttcttgtc attccaatca acgccgtcct ctatccgctg
     3121 gggatcggcc tcgtgtggtg gttcgccgct ccgcttgccc gctgcgtgcg gaaagtggcc
     3181 ggcggcggtt ccgcggatcc cgaagaattg cagcgagcac gggcccgcgc gatctcgctg
     3241 gggcatgtgg tggcagcgtt tggcttgggg ctgtgggtcg ttgccggtct ggcgtttccg
     3301 atcggcatga acatcatggt tggagtaggg cggtttgaca tttacgcgca cttctttctc
     3361 gccatgttta tcagcggcgt gatttcctgc tgtcttccct tcttggcgac gacctggctg
     3421 agcattcggg tcttctttcc ggcgctcctg gccggcagcg cgccgaacag tgcagagcat
     3481 cgcaggctgc aggcactcag ccgctatgca ggctggtaca tgtggctttc cccggtcggg
     3541 ccgctgggcg ccattttttt gatcgtcatc agcggcggcg aaacacaacg gcag
//
LOCUS       DASUGB010000136         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1126886, whole genome shotgun sequence.
ACCESSION   DASUGB010000136 DASUGB010000000
VERSION     DASUGB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126886"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1050)
                     /locus_tag="VFV87_02655"
     CDS             complement(220..1050)
                     /locus_tag="VFV87_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5102681.1"
                     /translation="MPINRVLEPEIMDSPLDAEDYDRMDHAAVNRVFVDDLLAALGEC
                     GVRNAECGMEAGDSVTPHSEFRTPHLLDVLDLGTGTALIPVELCRRCSARVSDPAEPA
                     DRRSPINVRVMAVDAATSMLDLARYNVEAAGLIEPVQLAHVDAKRLPYPDGMFDVVMS
                     NSILHHIPEPLTVLREAVRVTKPGGLVFLRDLLRPESLEQLNHLVVTYAADCNEHQRK
                     LFADSLHAALSLPEIRDLISQLGFPADSVQQTTDRHWTWTANRPEASARERISSADGL
                     "
     gene            complement(1040..2716)
                     /locus_tag="VFV87_02660"
     CDS             complement(1040..2716)
                     /locus_tag="VFV87_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX5102682.1"
                     /translation="MTHSRYNWAAKGDVNAFFGLMLDNVANLLLTVSLLHGAFAFPTE
                     VALRHMVPGTAMGVLVGDLLFFWLALRLAKQTGRDTITAMPLGLDTPSTFGMVFFVLG
                     PAFLQAKASGMDEHAAAIHTWHIGICAIFVSGLFKLVCAIGSNWIRRMFPRAGLLGSL
                     AAIALVLISFLPLLEILHSPIVGFVALAVVLTTLVARLDLPFKLPGAVGSLLIGGAMY
                     YAMQWATSMQLLPEVFALPPETHVIDPAKGLFPTEWLTAFRFEWLGALQESFHYLPIV
                     IPFALATVVGGIDCTESAAAAGDEYDTGQVIAIEALATLVASLCGGVIQTTPYIGHPA
                     YKAMGGKAAYVLATALFVGSAGLLGYFGFLYVIVPKVTIYPILIFIGLEITAQSFHHT
                     PTRHYPAVALACVPALATLGLMFVDQIFGDASIRGADLSITKLKDHLLIDNLTTLRIL
                     ANGFIVTSLMWSSALAALIDRRLWRAACFFLVCGGLTLFGIMHSPEPGAPMYFPWQLA
                     GDAQRHVFQFAAGYGVVALLLFGWGWLLKSQGVARVEDPPPVHEAHHHAD"
     gene            3128..3730
                     /locus_tag="VFV87_02665"
     CDS             3128..3730
                     /locus_tag="VFV87_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5102683.1"
                     /translation="MQSHSSRTDEELLLAYRKSHDADLFSELVKRYERELYSYLRRYL
                     GDAEMADDAFQAAFLQVHLKCEQFEAGRSVRPWLYTIATNQAIDLQRRSKRHRMVSLD
                     RSTTSDGEDVGKLLDLLTSKEVDPTDQLTADERRQWLHRAVEQLPQPLAEVVNLVYFH
                     DLKYREAADALNIPVGTVKSRMHSAVAKLNEAWTTAQAPP"
     gene            3757..4749
                     /locus_tag="VFV87_02670"
     CDS             3757..4749
                     /locus_tag="VFV87_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102684.1"
                     /translation="MSPTREHLLGYLLGALERTEQEQVEAELEHNPALRAELQRLQDC
                     IGRVGLVCAGPEKEPEHFDPPADLAVRTCRLVAERHERAVVITRQSWGAVSTDGSQRQ
                     LTWVNVLTAASVLIAAAALFFPALNHSLGQAEIAYCQNNLRQLGVALHEYSSLRPDGS
                     FPRIEMAGNRSVAGMYAPILLHNQHVDNPRVFLCPSSERSRRGVKLHIPTPEEIDKAT
                     GEMLQEYQATIGGDFGYNMGFRRNGEVVAPCNQSRNSYVLLSDAPSDSLPGRRSANHA
                     GVGQNVLCEDGHVKFIKSFPCPQFPDDPFYNLERRVAAGLTCDDHVVGASSDRP"
     gene            4887..5792
                     /locus_tag="VFV87_02675"
     CDS             4887..5792
                     /locus_tag="VFV87_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5102685.1"
                     /translation="MNRSVLTTLLIILIWHGSTASADDKHAIKHPVLDVWPGKVPGET
                     KEIGKEGFREPRASEKQDVLRLENVSVPTLTVYDAPAEKKNGCAVIVAPGGGYNILAW
                     DLEGTEVADWLNTLGVTAFVLKYRVPNREGYANNEPPLMDAQRAVSLVRSKASEFGID
                     PARLGMLGFSAGGNLTAKTCLMSETRAYDKLDTVDEVSCRPDFGVLVYAAYLVDESGK
                     VRPELKVDAKSPPMFFAHALDDRVSPENSIGLLLALKAAGVPSELHLYDSGGHGFGLR
                     KSAFPCHTWPDRCGEWLEHRGLLKK"
BASE COUNT         1147 a         1845 c         1955 g         1002 t
ORIGIN      
        1 gttccgacca ggaacgacgc cgacgaaaag tccgctctcc gtaatgggcc actaccggtg
       61 aagagcgcga ccgatcccca gagcaaggac gccgtggccg acacggtcaa tagtaccgcg
      121 ggcgttctga ggcggcggac cgcctcgttg cgagtcagcg gcgcgctcgc ttgcgaaggt
      181 atctcgacag gtgccgcgta cggattgttg cggtcatagt cagaggccat cggctgatga
      241 tatgcgctcc ctcgctgacg cttcgggtcg gtttgcagtc catgtccaat gccggtcggt
      301 agtttgttgg acgctgtcgg ccggaaagcc gagttgggag atgaggtcgc ggatttcggg
      361 gagggagagg gcggcgtgga gggagtcggc gaagagcttt cgctggtgct cgttgcagtc
      421 ggcggcgtag gtgacgacga ggtggttgag ctgctccagg ctctcggggc gcagcaggtc
      481 gcggaggaag acgaggccgc cgggtttggt gacgcggacc gcttcgcgga ggacggttag
      541 cggctcgggg atgtggtgca ggatgctgtt ggacatgacc acgtcgaaca tcccgtcggg
      601 gtagggcaaa cgcttggcat cgacgtgggc gagctggacg ggctcgatga ggccggcggc
      661 ttcgacattg tagcgggcca gatcgagcat gctcgtcgcc gcgtcgacgg ccatgacgcg
      721 gacattgatc ggagacctgc ggtcggccgg ctcggcgggg tcggagaccc gtgccgagca
      781 gcggcggcag agttcgaccg ggatgagggc cgtgccggtg ccgaggtcga ggacgtcgag
      841 caagtgcgga gtgcggaatt cggaatgcgg agtgacagaa tcgcctgcct ccattccgca
      901 ttccgcattc cgcactccgc attcccccaa cgcggccaat agatcgtcca caaacacgcg
      961 attgaccgcc gcatggtcca tccggtcgta gtcttcggcg tccagcggcg agtccatgat
     1021 ttcgggctcg aggacgcggt taatcggcat gatggtgggc ctcgtgaacg ggcggcgggt
     1081 cttcgaccct tgcgactcct tgcgatttta gcagccagcc ccagccgaag aggagcagcg
     1141 cgacgacgcc gtagccggcg gcgaactgga agacgtgccg ctgggcgtcg ccagccagtt
     1201 gccaggggaa atacattggg gcaccgggct cgggggagtg catgatgccg aagagcgtca
     1261 gtcccccgca aaccaggaaa aagcaggcgg cccgccagag ccggcggtcg atgagggcgg
     1321 cgagcgccga ggaccacatc aggctggtga cgatgaagcc gttggcgagg atgcggagcg
     1381 tggtcaagtt gtcgatcagc aggtggtctt tgagtttggt aatcgacaga tcggcgccgc
     1441 gaatcgatgc atcgccgaag atctgatcga cgaacatcag tcccagcgtc gccagcgccg
     1501 gcacgcaggc cagcgcgaca gccggataat gccgcgtcgg cgtgtgatgg aagctctgcg
     1561 cggtgatttc caggccgatg aaaatcagaa tcggataaat agtgaccttc ggcacgatga
     1621 cgtagagaaa gccgaagtag ccgagcagcc ctgcgctgcc gacgaagagg gcggttgcca
     1681 gcacgtaagc cgccttgccc cccatcgctt tgtaagccgg atggccaatg tacggcgtcg
     1741 tttgaatcac gccgccgcac aggctggcga caagcgtggc caacgcttcg atggcgatta
     1801 cctgaccggt gtcgtactca tcgccggcgg cggcggcgct ttcggtgcag tcgatgccac
     1861 cgacgacggt cgccagcgca aagggaatca cgatcggcag gtagtgaaac gactcctgca
     1921 gtgcgccgag ccactcgaag cggaatgcgg tgagccattc tgtcggaaaa agtcccttgg
     1981 ctggatcgat gacgtgcgtc tcgggcggca gcgcgaagac ttccggcaag agctgcatgc
     2041 tcgtcgccca ctgcatcgcg tagtacatcg ccccgccgat caacagcgag ccgaccgcgc
     2101 cgggcagctt gaacggcaag tcaagccggg cgacgagcgt cgtcagcacg acggccagcg
     2161 cgacgaagcc gacgatcggc gaatgcagaa tttcgagcag cggcaaaaag ctgatgagca
     2221 ccaatgcaat cgccgctagc gagccgagga gcccggcccg cgggaacatc cgccggatcc
     2281 agttcgagcc gatggcacag accagcttga acaatccgct gacgaaaatc gcacaaatgc
     2341 cgatgtgcca cgtatgaatg gcagcggcgt gctcgtccat gccgctggct ttcgcctgca
     2401 ggaacgccgg gccgagcacg aagaagacca tgccgaacgt gctgggcgta tcgagtccca
     2461 gcggcatggc ggtgatggta tcgcggccgg tctgcttcgc cagccgcagc gccagccaaa
     2521 agaagagcag gtcccccacg agcacgccca tggcggtgcc gggcaccatg tggcggagcg
     2581 ccacttctgt aggaaatgca aacgcgccgt ggagcaagct gaccgtcagc agcaggttgg
     2641 cgacgttatc taacatcaag ccgaaaaagg catttacgtc gccttttgcg gcccagttgt
     2701 agcggctgtg cgtcaccgaa ggtccgttgg cacgggcttt gcttttgggt gcccgcgagt
     2761 gtaggacgcc cctgggcgac tgtcaaatcg gctcattcga agggccaatc gcccgcggga
     2821 atgccagcat ggagcgccgc atgaacgcgc aaattggagc tgatccgtcc acgccctcac
     2881 gagccaaaca ttgctggctg tggagcgtgg acggatcgga agtccaatct gcgccacgct
     2941 ttgcactcgc tccggcgcct ctggcggcag acttttccag ccgcagccgg taaaatggca
     3001 gtttgctcgc gccagcactg cgcgcgggca tccgcagagg ccgctccctt aaaaaccagc
     3061 gaactaaccg ccctgcggac cgtagaaaac gatgaaagcc actttgccag agccgtcggt
     3121 tgggcctatg cagagtcact cttcgcgaac cgacgaagag ttgctgctgg cctatcgcaa
     3181 atcgcacgat gcggacctgt tctccgaact ggtcaagcgg tacgagcgcg agctgtatag
     3241 ctacctgcgg cgctacctgg gcgatgcgga gatggcggac gacgccttcc aggccgcgtt
     3301 cctccaagtg catttgaagt gcgagcagtt cgaggccgga cgctccgtgc ggccgtggct
     3361 gtacacgatc gccaccaacc aggcgatcga cttgcagcga cggagcaagc ggcaccggat
     3421 ggtgagcctc gaccgcagca cgacgagtga cggcgaagat gtcggcaaac tgctcgactt
     3481 gctcaccagc aaggaggtcg atccgaccga tcagctcact gcggacgagc gccgccagtg
     3541 gctgcaccgg gcggtcgagc aactgccgca gccgctggcc gaggtggtga acctggtgta
     3601 tttccacgac ctgaaatacc gcgaagcggc cgacgccttg aacatccccg tggggacggt
     3661 gaagagccgg atgcactccg ccgtcgccaa gttgaacgaa gcctggacca ctgcccaggc
     3721 gcccccctaa ccgatcctgc cagaagctaa ccagccatga gcccgacccg cgagcacttg
     3781 ctcggctact tgctcggagc gcttgagcgc acggaacagg agcaagtcga agctgaactc
     3841 gaacacaatc ctgcccttcg cgccgaattg cagcggcttc aggactgcat cggccgcgtg
     3901 ggtctggtct gtgccggccc tgagaaggag ccggagcatt ttgacccgcc tgccgatttg
     3961 gcagtgcgaa cttgccgcct ggtggccgag cgccacgagc gggcggtcgt gatcacgcgt
     4021 cagagctggg gtgctgtctc gaccgacggc agccagcggc aattgacctg ggtcaacgtc
     4081 ctcaccgctg cctcggtcct catcgccgcg gccgccctgt tcttcccggc cctgaatcat
     4141 agcctgggcc aggccgaaat cgcctattgc cagaacaatc tgcggcagct tggcgtggcg
     4201 ctgcacgaat acagttcgct gcggcccgac ggcagctttc cgcggattga aatggcgggc
     4261 aaccgcagcg tggcgggcat gtacgccccg attctcctgc acaaccagca cgtcgataat
     4321 ccgcgcgtgt tcctctgccc gtcgtcggag cggtcgcgcc gcggcgtgaa gctgcacatc
     4381 cccacgcccg aggagatcga caaggccacc ggcgagatgc tccaggaata tcaggcgacg
     4441 attggcggcg acttcggcta caacatgggc tttcgacgga atggcgaagt ggtcgcaccc
     4501 tgcaaccaga gccgcaactc gtacgtcctg ctttccgacg ccccgagcga ttcgcttcct
     4561 ggccgccgct cggccaatca cgcgggagtc gggcagaatg tcctgtgcga ggacggccac
     4621 gtgaagttca tcaagtcatt cccctgcccg cagtttccgg acgatccgtt ctataacctc
     4681 gagcgccgcg tcgccgccgg cctgacctgc gacgaccacg tcgtcggcgc aagcagcgac
     4741 cggccgtagc ggcgcgaaat gttcggttgg gcgcgcgaac cgcatactcg cgctcttttc
     4801 cgcgcgcatc tcgcgcaatc catcgacatt cctattgaag tcgaatagga tttctccgtt
     4861 ccgcccatcc ggcgtaagga gaatccatga accgttctgt gctcaccacg ctgctcatta
     4921 ttctgatttg gcacggctcg accgcgagcg ccgacgataa gcatgccatt aagcaccccg
     4981 ttctcgacgt ctggccgggg aaagttccgg gcgagacgaa ggagatcggc aaggagggct
     5041 ttcgcgagcc gcgggccagc gaaaagcagg acgttctgcg gctcgagaat gtgagcgtgc
     5101 cgacgctgac ggtctatgat gctccggccg agaagaagaa cggctgcgcg gtgattgtcg
     5161 cgccaggtgg cgggtacaac attctggcct gggatctgga agggacggaa gtcgccgact
     5221 ggctgaacac gctcggcgtg accgcgttcg tcctgaaata ccgcgtgccg aaccgcgagg
     5281 gctacgccaa caacgagccg cctctgatgg atgcccagcg agccgtcagc ctggtccgca
     5341 gcaaagccag cgaattcggc atcgatccgg cacgcctggg catgcttggc ttctcggccg
     5401 gaggcaatct tacggccaaa acgtgcctga tgtcggaaac gcgggcctac gataagctcg
     5461 acacagtcga tgaggtctcc tgccgccctg atttcggcgt gctcgtctat gcggcgtacc
     5521 tcgtggacga aagcggcaag gttcgtccgg agctgaaggt cgatgccaag tcgccgccga
     5581 tgttcttcgc ccacgccttg gacgaccgcg tcagccccga gaacagcatc ggcctcctcc
     5641 tcgccctcaa agccgctggc gtgcccagcg agctgcacct ctacgattcc ggcggccacg
     5701 gcttcgggct gcggaagagc gcgttcccct gccacacctg gcctgaccgc tgcggcgagt
     5761 ggctggagca ccgcgggctg ctcaagaaat aatagacgca ctcaagaagc gcggcgttac
     5821 ccggtccgtt gatacggccc ccgcccgcgg ccatttcctg cggtcggctt ggggggcgtc
     5881 gtgatcagcc gcagatttcc cggtccgagc agatcgtcca gccgttgacg cagctcgcgc
     5941 gtgacttcc
//
LOCUS       DASUGB010000137         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1961782, whole genome shotgun sequence.
ACCESSION   DASUGB010000137 DASUGB010000000
VERSION     DASUGB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961782"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFV87_02680"
     CDS             complement(<1..213)
                     /locus_tag="VFV87_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5102686.1"
                     /translation="MTTTVRKPPGPRGHWLLGALPRMQRDMLGFFEECHREYGDAAYF
                     RMPGRRTMLLSHPDDIEQVLVTENRRF"
     gene            complement(687..1277)
                     /locus_tag="VFV87_02685"
     CDS             complement(687..1277)
                     /locus_tag="VFV87_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102687.1"
                     /translation="MIRLAIFLGTALITVAFAQAALTEDNPTGAPASKGATDMEFEFH
                     RGKVPATDLKIGLASAKQAVQERRGLVLPLTISNASDKTIKTRLAHEWHGGEWPPTSL
                     FASVTPTDDKRPKPFVPVYLAGEDQAVPRQVSLAAGKSIDIELRMDWPGTGSVKGDPL
                     MSKPGKYVAKFALVFEVDGKQQHALSAPIEVEYKAE"
     gene            complement(1447..2106)
                     /locus_tag="VFV87_02690"
     CDS             complement(1447..2106)
                     /locus_tag="VFV87_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102688.1"
                     /translation="MTSAANPPCPRSWLRLQFSLGTLFWLMLFAGMAAVWWRDRSSLD
                     ARIKKLEEMYAPQREILWGAADILGAPNDPMGTAGKSWCPAGTNTADWVEVGFDSSVR
                     AATIDLYETYLVGCVTEVLVLDGSGNEVRLWKGTDPTPQLPRTGLFKVPVPPSIRSIE
                     AVKIRVDSTGKGSWACLDAVGLTTAAGNTTWATSANCSSVYGGSSLTASIERKPWYRL
                     W"
     gene            complement(2318..2764)
                     /locus_tag="VFV87_02695"
     CDS             complement(2318..2764)
                     /locus_tag="VFV87_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102689.1"
                     /translation="MNEVSRRSQMALSAVGLLLAANVVVPLLWGDVYPFTSAPMFRDA
                     PARCCNYRVFASNGSELPAEDWLVQRIYDGNPVGYGVGICPPEILERFGEECDEATVR
                     RHIARQFAQPQNCDFEYVEVVQELIGPVDAQQVGVVRSDRWRVDRP"
     gene            complement(2932..3555)
                     /locus_tag="VFV87_02700"
     CDS             complement(2932..3555)
                     /locus_tag="VFV87_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5102690.1"
                     /translation="MSVLRFDCRFHYPAGFSLEFAFEADSGVTALVGPSGCGKTTVLN
                     LIAGLLRPDEGQIVLAEQELFSSTSGTNLPPERRQVGFVFQDYQLFPHLTVAQNLRYG
                     ERRSRSVRFSFAKTVEILGLGGYLDRHPVSLSGGQRQRVAIGRAVLRGANLLLLDEPF
                     SALDAELRQDVSGYLGQVIAEFQIPTLLVSHHPESVSGLASSTVRLS"
     gene            complement(3552..4022)
                     /locus_tag="VFV87_02705"
     CDS             complement(3552..4022)
                     /locus_tag="VFV87_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5102691.1"
                     /translation="MLEHTLGLRFVFDWKGAALASAVVGFPLFVRPIRLAFAAVDSRL
                     VQAARTLGAGPWDAFWSIMLPLARPGILSGCILAFARSMGEFGATIMIAGNIPGRTRT
                     IPLLIYSELDAPGGIEASLRLVVVSVVIAAAALFAGELLDHYGRRRLSAADQPA"
BASE COUNT          788 a         1323 c         1189 g          739 t
ORIGIN      
        1 gaaccggcgg ttctcggtca ccagcacctg ctcgatgtcg tccggatgcg agaggagcat
       61 cgtccgccgc cccggcatcc ggaaataagc cgcatcgccg tactcgcggt ggcactcctc
      121 gaaaaaaccg agcatgtccc gctgcatccg cggcagcgct cccagaagcc aatgtccccg
      181 tggtcccggc ggcttccgca ccgtcgtcgt catcgctgtt ccatcctctc ccaaagccgc
      241 gaccagaggt cgcggctcct ggctgcccct ccgatctcca cggtctccga tcccgccttt
      301 gattaggaca ggcatctcgt cgcggagaag ggggtgtgga ccgcgcgacc gagcaacttt
      361 ccagtttttt gtcgctcaat atccctgcgc gatagtctct caactcccat tccaccaatg
      421 acttgcgccg cgcaacaccc cccatgggag gatgttgcgc tgttgcgcgt cgtcaaccgt
      481 tgttcctccg attgtgtgat cgaccgcggt gctgcgccgc atcccacaac tttccctgtc
      541 tgggcgcgca ccccgaccgc cacgatttat cccaattgta cacatgacgc ctctttccgc
      601 caatccctca accgggtgca actcgcgaaa tttcggctga ggcgctcgcg cctccttgac
      661 gcgcgattct catggatgcg ggggcctcac tcggccttgt actcgacctc gatcggcgcc
      721 gatagagcgt gctgctgctt accgtctacc tcaaacacca gggcgaactt cgcgacgtac
      781 tttcctggct tgctcatgag cgggtcgccc ttcaccgagc cggttccggg ccagtccatt
      841 cgcagctcga tgtcgatgct tttgccagcc gcgagcgaga cctggcgtgg aacagcctgg
      901 tcttccccgg ccaggtacac gggaacgaag ggcttgggcc gtttgtcgtc cgtcggcgtg
      961 acgctggcaa acaacgatgt cggcggccat tcgcccccgt gccattcgtg ggccagcctc
     1021 gttttgatcg tcttgtccga ggcattcgag atcgtcagcg gcaggaccag cccccgcctc
     1081 tcctgcacgg cctgtttggc ggaggccaat ccgattttga ggtcggtagc cggcaccttt
     1141 ccacggtgaa actcgaattc catatcggtc gcgcctttcg atgctggcgc tccagtcgga
     1201 ttgtcttcag ttaaggccgc ttgggcaaaa gcgaccgtaa ttagcgccgt cccgaggaaa
     1261 attgcgagtc gaatcattgg cgtgctccaa ctgatggaat ggatttgaac aaatcggtca
     1321 tcggttagac ggtcacgcag ctccgttggt ttgcacaccg ttgcctgccc gctctcccac
     1381 cgaacgaatg tgcttatgcc aagcggcggg agaagtgaag gtcccaatcg tttggccatt
     1441 tttcccttac cacagccggt accacggctt cctctcaatc gaggcagtga ggctgctgcc
     1501 gccatagacg ctgctgcagt tggcggaggt ggcccaggtg gtattgccgg cggccgtggt
     1561 gaggccgacg gcgtcgaggc aggcccagga gcccttaccg gtactatcga cgcgaatctt
     1621 taccgcctca atcgaccgaa tcgacggagg gaccggcact ttgaagaggc ccgtgcgtgg
     1681 caactgcggc gtaggatcgg tccccttcca aaggcggacc tcattgccgg agccatcgag
     1741 cacgagcacc tccgtcacgc agcccacgag gtaggtctcg tagaggtcga tcgtggctgc
     1801 gcgcacggac gaatcgaagc cgacttccac ccagtcggcc gtgttggtcc ccgcggggca
     1861 ccacgatttt cccgccgtgc ccatcggatc gttgggcgcg ccgagaatat ccgccgctcc
     1921 ccataagatc tcccgctggg gcgcgtacat ttcctccagc ttcttgatcc gtgcatcgag
     1981 gctgctgcgg tcccgccacc agacggccgc catccccgcg aagagcatca gccagaatag
     2041 ggtccccaaa ctgaactgca gtcgcagcca ggagcgggga cagggcggat tggcggcaga
     2101 agtcattggt cgaaaatctc cgaggcgaag tgcgccatca ccgtccggta acggttgcgc
     2161 caacgggcgg tggatgcgtc tgcggcgaaa tcattcgcgc cgcgccagca ggccagacac
     2221 gctgaaaatt ggcgacattc aacgtcacca ggtagtggat tgcagacccc gttgcggatt
     2281 ttgacaaagg gcaggccgca atgcaaaccg aagagcctca gggccgatcc acccgccagc
     2341 ggtcgctgcg gacaacgccc acctgctggg catcaaccgg cccgatcaat tcctgcacaa
     2401 cttcgacgta ctcgaaatcg cagttttgcg gctgggcaaa ttgtcgggcg atgtgccggc
     2461 gaacggtcgc ctcgtcgcat tcctcgccaa accgctccag aatttccggc gggcaaatcc
     2521 ccacgccgta gccgacgggg ttgccgtcgt atatccgctg gacgagccaa tcctcggccg
     2581 gcaactcgct gccattcgag gcaaagaccc ggtaattgca acagcgcgct ggcgcatctc
     2641 taaacatggg cgccgatgtg aagggataca catcgcccca caacagtggc acaacgacat
     2701 tggccgcgag cagcaagccg acagcggaaa gtgccatctg agaacggcgc gaaacctcgt
     2761 tcatctccag attatagccg cacctcggtc gcaaggttcc aatcgacgcc gtgctctcta
     2821 cggatttcct cccaaagcgt ccggcaaaga gcccttggcg atcgcggcgc tcgccagcgg
     2881 cttgttgatc cgccgcacag ccggaatcca gattggcctg ttcgctgacg atcagctcag
     2941 ccgcacggtc gaactcgcca gtccgctcac gctctcgggg tgatggctga cgagcagtgt
     3001 gggaatctga aactcggcga tcacctgccc aagatagccg cttacgtcct ggcgcagctc
     3061 ggcatccagg gcgctgaagg gctcgtcgag cagcagcaaa ttggcgccgc ggagcacggc
     3121 gcggccaatg gcgacgcgct gcctttgtcc accgctcagt gaaaccgggt ggcggtccaa
     3181 atagccgccg aggcccagga tttccaccgt cttggcgaag ctgaaccgga cgcttctgga
     3241 ccgtcgctct ccatagcgca agttctgcgc taccgtcagg tgtgggaaga gctgatagtc
     3301 ttgaaagaca aagccgacct ggcgacgctc gggaggcaga ttcgtcccgc tcgtcgagct
     3361 gaatagctct tgctcggcga ggacgatctg gccctcgtca gggcgcaaga ggccggcgat
     3421 taaattcagg acggtcgtct tgccgcagcc ggatggaccc acgagcgccg taactccgct
     3481 gtcggcctcg aacgcaaact ccagcgagaa acccgcggga taatgaaatc ggcagtcgaa
     3541 tcgcaggacg ctcatgcggg ttggtccgcc gcggaaagcc gccggcgtcc gtaatgatcg
     3601 agcagttctc cggcaaacag agctgccgca gcaatgacga ccgaaaccac gaccaggcgc
     3661 agcgaggcct cgatgcctcc cggggcatcc agctcggaat agatcagcaa aggaatcgtg
     3721 cgggtccggc cggggatatt tcctgcaatc atgattgtcg cgccgaattc gcccatgctc
     3781 cgcgcaaatg ccagaatgca gccgctcagg attccaggtc gagctaaggg cagcatgatc
     3841 gaccagaacg catcccaagg ccccgcaccg agcgtgcgag ctgcttgcac gaggcgcgag
     3901 tcaacggccg cgaatgccag ccgaatcggt cgcacaaaaa gcgggaaccc gacgactgca
     3961 gaggccagcg cggcgccttt ccagtcaaac acaaaccgga gtccaagcgt gtgctccagc
     4021 aacccgccta cgaggccgt
//
LOCUS       DASUGB010000138         6015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_850247, whole genome shotgun sequence.
ACCESSION   DASUGB010000138 DASUGB010000000
VERSION     DASUGB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850247"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFV87_02710"
     CDS             <1..638
                     /locus_tag="VFV87_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102692.1"
                     /translation="ELYSRFPRRRLEGEAIRDAMLSAAGLLTGERGGPGIMPPLPDEL
                     VNTLLKGQWTTSKREADHYRRSVYVFARRNLRYPIFEAFDRPDANASCPARNRSTTAP
                     QALTLLNSEFTLLCSRHIAGRILRETAEPDEQVKRLYELTYARQPSPEELQLLKVFLA
                     TQREALLVERRAGNELVLPAGCPDVVDPPAAAALVDACLAMVNASEFLYVD"
     gene            783..1217
                     /locus_tag="VFV87_02715"
     CDS             783..1217
                     /locus_tag="VFV87_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102693.1"
                     /translation="MSAMWNKVLVAALALSAIGFIAGSGSISIGQEAKAQKTEKKAKG
                     RLPAYFADIVTDDQRTAIYKIQASYKKQIDDLESQLTALRDKEAAEIEGVLDAEQKEK
                     LKAAREAAAAKKKKKATSEKTTEAGTTTPPAAKTAKTAAKTQ"
     gene            1362..1443
                     /locus_tag="VFV87_02720"
     tRNA            1362..1443
                     /locus_tag="VFV87_02720"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1393..1395,aa:Ser,seq:cga)
     gene            complement(1790..2077)
                     /locus_tag="VFV87_02725"
     CDS             complement(1790..2077)
                     /locus_tag="VFV87_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX5102694.1"
                     /translation="MHRIKPNLTAEAGSTIPTKGVIPMLVLSRKENERLIIDGNVVVT
                     VVRVAGGKVRLGIEAPTNVHVKRQELIDRPMSGDLRSAVRRSPSAAPSPRT"
     gene            2630..3892
                     /locus_tag="VFV87_02730"
     CDS             2630..3892
                     /locus_tag="VFV87_02730"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEX5102695.1"
                     /translation="MATARDGRYPAIHISPVVCQMRISGFFRRNPFVGDDPLSAYQRY
                     QELQGYVSWTPEDAQRVQAIAPLVTPHFTALVDDFYAEIRRHPDAAKVITGGEEQINR
                     LKKTLIRWLEELFSGCYDREYVERRWRVGYRHVEIGLDQVYTNVALSRLRRGLLLAIE
                     RDWKESIPDLLAIRASLNTLLDLDLAIIEDAYQTEFQRRQAAAERLATIGKVAGGIAH
                     ELRNPLNVVKTSVYFILNAKNASPEKIKTHLQRIERQVGVADGVITALNDFSRLPVPQ
                     MDRVDLESCLKDVLEVTTLPRNLQVEWALGSPSPAVLGDRAQLHIVFSNLIRNARDAM
                     PEGGTLRFAAASNRGHVDIQVQDTGIGIPAEDLSRIMEPLYSTKAKGIGLGLSIAQEI
                     VGRHNGSLRVASQQGVGTTFTIRLPRAS"
     gene            3905..4645
                     /locus_tag="VFV87_02735"
     CDS             3905..4645
                     /locus_tag="VFV87_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5102696.1"
                     /translation="MVDYTHRILIVDDEADTCANLSDILTDLGYQVDTANDGFAALEL
                     VKENAYDIALLDLRMPGMDGVQLYRRIREISAGTVAIVVTAYASSDTARSARAAGAWQ
                     VLPKPVHIGNLLGLVSEALDAPLILVVDDDCELCENLWDVMREHGYRVHLAHDVPDAE
                     QAIQRHAFQVVLVDMRLPSGDGGQVLRLLRQKDQSARALIITGFASEMERRVQEALAA
                     GATAVCYKPFDVEKLLTTVHDLCKPPAN"
     gene            4703..>6015
                     /locus_tag="VFV87_02740"
     CDS             4703..>6015
                     /locus_tag="VFV87_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5102697.1"
                     /translation="MLVIDDDADTRENLCDILELDGYRYEAAGTASELLERSNWDDVA
                     LVLLDRKLPDGSPQEILPRLKVLAPQVAVIIVTGYADIEGAISALQSGAADYILKPIN
                     PDALRASVRRVIQRQRDLQQIACLSSDLEESEVRYRALFENTMDGLVILDDESRIFAA
                     NPAARSMLGWTGGASEMKLESLKLDNEGQMNAATRGDFLGPGKHSGEYWIAVPGGRIA
                     IEYRAVENSAPGLHLISMRDVTARKCAEERARQAERLAAIGETMTALVHESRNALQRS
                     KASLELLKLEVEDRPEATKLANRVERAQEDLHQLFEEVRQWAAPLKLRREVCNLRELW
                     QETWCLVGQANPARKAKLVEELTCEPICHADEFALGQVFRNIFENAIEVAPPGSNVTI
                     RCAHPPSGNGNVIVLTIRDEGPGLTAEQQQRIFEPFFTTKAKGTG"
BASE COUNT         1228 a         1768 c         1841 g         1178 t
ORIGIN      
        1 atgagctcta ctcccgcttc ccgcgccgcc gcctcgaggg cgaggcgatt cgagacgcga
       61 tgctctcggc ggcaggtttg ctgaccggcg agcgcggcgg gccgggcatc atgccgccac
      121 tgccggacga gctggtgaac acgctcttga aggggcaatg gacaacgagc aagcgcgagg
      181 cggaccacta tcgccgcagc gtgtatgtgt tcgcgcgccg gaatctacgg tatccgatct
      241 tcgaagcctt cgaccggccc gatgccaacg cgagctgccc ggcccgcaat cgctcgacca
      301 cggcgccgca agcgctgacg ctcttgaaca gcgagttcac gctgctctgt tcccggcaca
      361 tcgctggccg catcctgcgc gaaacggcgg aacccgacga gcaggtcaag cggctgtatg
      421 aattgaccta tgcccgccag ccatcgcccg aggagcttca gctcttgaag gtctttttgg
      481 cgacgcagcg agaggcgctg ctcgtcgagc gtcgagcggg caacgagttg gttttgccgg
      541 cgggctgccc ggacgttgtc gatccgcccg cggcggcggc cctcgtcgat gcctgcctgg
      601 ccatggtgaa tgcgagcgag ttcttgtacg tcgattgaat cgctcggcag agggcacgat
      661 tctgcaaagt tgatgcttga ctttgggccg cgcgcaccgc taccttgtgc aacgcacatg
      721 gagggtgata ggccccacaa ggaagcctgt ttttagccca attcaaccgg agatttcgac
      781 tcatgagtgc catgtggaac aaggtgctgg tggctgccct ggcgttgtcg gcgatcggat
      841 tcattgctgg ttcaggtagt atttcaattg gacaggaggc gaaggctcag aagacggaga
      901 agaaggccaa aggacgcctg ccggcgtact ttgccgacat cgttacggac gaccagcgca
      961 cggcgatcta caaaatacaa gcgtcgtaca aaaagcaaat cgacgacctc gaatcgcaac
     1021 tcaccgcact tcgcgataag gaagcggctg agatcgaagg cgtcctggat gccgagcaga
     1081 aggaaaagct gaaggcggct cgcgaggcgg cggcggccaa gaaaaagaag aaggccacta
     1141 gcgaaaagac gaccgaagcc ggcacgacaa ctccaccggc ggcgaagacg gccaaaaccg
     1201 cggcgaagac gcagtaggtt taggcctgct tccggctgat aggaggattc gctgtcccag
     1261 cgcgccgcaa attgcggcgt gcttgcaaaa cttggttcgc aacgtccttc cggtccaaac
     1321 gcggcttgcc tgctatttcg gcgcaggcta gatttgacct tggagggtaa gcgaatggct
     1381 agcagactga ttcgaaatca gttgccgggc aaccggttgc gggttcgagt cccgtgccct
     1441 ccgcttcaaa agagcggcgc ttcgtaagtg ctgaattggc agcggcctgc gaaacgcccc
     1501 tcgcttcgag gcagcctgtc gtcccagatt tgtccccacc ttgcggtcgg tgaaaatcgg
     1561 ggcagaggcg attttggttg cgccgccgtg gaggatggtc gcacgggctc gggccttctt
     1621 tcgctcgtcg gagaattcga gcagtgcaac gagcttgtcc tctgccgtct gggccagcgc
     1681 ctggcggaag gcctcgacgg aggtattccc ccttcgaagt tgaccacgtt cagcccggcg
     1741 cccgaaagcc tttcgcaggc ccgcctgtcg cggctgcccg aacggcaaat cacgtacggg
     1801 gactcggtgc cgcacttgga ctgcggcgaa ccgccgagcg cagatcgccg ctcatcggcc
     1861 tgtcgatgag ttcctgcctt ttgacatgca cattcgttgg cgcttcgatg cccaggcgaa
     1921 ctttgccgcc tgccacgcgg acgactgtga cgacgacgtt tccgtcgata atcaatcgtt
     1981 cgttttcttt ccgtgagagc actaacatgg gaatgactcc tttcgttggg attgttgaac
     2041 ctgcctcagc ggtcaagttc ggcttgatgc gatgcaactt cgatctcgct caggacacat
     2101 ccgagtccga cgacattcgc ggccagtgtg tccgcaactt gtcttaggta gaacgacggg
     2161 agagttccgc gaacgatcac ttggtcctgt tcgcaaacgc aaacgagttg cctcagcggc
     2221 caatagggac tgcggcgaaa gcgctcctcc aagtcggttg ctgcggaagg cggcgctggc
     2281 gccttcatct gggcggagcg ttgaaccatt ggtgaatctc cagcacgaag tgaacgtgcg
     2341 atcgatggcg gaacatgagg cagcacgcct cgaagatggc ttgcctgtgg gaagagagtc
     2401 cgtcacgaac tcgggcaagc caaatctagt tgcaacctgc cggtcgccgg tcgcgcccaa
     2461 tcacgagcac gagagtaacg ctaatgtgat cccaacttgg ggccatgtca acagcggttt
     2521 tgccagacca attgcagttt tctctccatt tttgaatgcg gccgggatct attcctccaa
     2581 atcgcgccgg aatctttccg ggctggggtg cggctgctat gatgacggcg tggccaccgc
     2641 tcgcgacggg cggtatccgg caatccatat cagcccggtg gtctgccaaa tgcggatcag
     2701 cgggtttttt cgtaggaatc cgtttgtcgg agacgatcca ctgtccgcgt accagcgcta
     2761 tcaagagttg cagggctacg tctcctggac gcctgaggac gcccagcgcg tgcaagccat
     2821 tgctcccctg gtgacgccgc actttaccgc gctcgtcgac gatttctacg cagagattcg
     2881 gcggcaccct gatgccgcca aggtcattac cggcggcgag gagcagatca atcgcctgaa
     2941 gaagacgtta atccgctggc tcgaggaatt gttctccgga tgctacgacc gcgagtatgt
     3001 cgagcgccgc tggcgggtcg gctaccgcca cgtcgaaatc ggcctcgatc aggtctatac
     3061 caatgtcgcg ctctcacgac tgcggcgcgg cttgctcctg gccatcgaaa gagattggaa
     3121 ggaatcgatc cccgacttgc tcgccattcg ggcctcgctg aatacgctct tggatttgga
     3181 cctcgcgatt atcgaggacg cctaccagac cgagttccag cggcgccaag ctgccgccga
     3241 acggctggcc acgatcggta aggtcgccgg tggcatcgca catgaactgc gaaatcccct
     3301 caacgtggtg aagacctccg tttacttcat ccttaacgcg aagaacgctt cccctgagaa
     3361 gatcaagacg catctccagc gaatcgagcg ccaggtagga gtggccgacg gtgtcatcac
     3421 ggccctgaac gacttctcgc ggctgcccgt tccacaaatg gaccgcgtgg acctggaatc
     3481 ttgcctgaaa gacgtgttgg aagttaccac acttccgcgc aatttgcagg tcgaatgggc
     3541 tctcggctcg cccagcccgg cagtcctggg cgaccgcgca cagcttcaca tcgtgttcag
     3601 caatctcatc cgcaatgccc gcgacgccat gcccgaggga ggcacgctcc gcttcgcggc
     3661 cgcttccaac cgaggccacg tcgatattca ggtgcaggac actggaatcg gcatacctgc
     3721 cgaggatttg tcccgaatca tggagccgct atactcgaca aaagccaaag gcattgggct
     3781 cgggctttcg atcgctcagg agattgtcgg ccggcacaac ggcagcctgc gcgtggccag
     3841 ccaacaaggc gtaggaacaa ccttcacgat tcgcctgcca agagcgtcct agacgcagaa
     3901 aaatatggtg gattacactc atcggattct gatcgtcgat gacgaggcgg acacctgcgc
     3961 taatctgtcg gatattctca ccgatttagg ctaccaggtg gacacggcca acgacggctt
     4021 cgcggcgctg gagctggtca aggagaacgc gtacgacatt gccctcctcg atttgcggat
     4081 gccgggcatg gatggcgtcc agctttaccg ccggattcgc gaaatctcgg ccggcaccgt
     4141 ggcgattgtg gtaacggcct atgccagcag cgacacggcc agatccgctc gcgcagccgg
     4201 cgcctggcag gtccttccca agccggtcca tatcgggaac ttgcttggcc tggtcagcga
     4261 ggcgctcgat gcgccgctga ttctggttgt cgatgacgac tgtgaactct gcgagaacct
     4321 ttgggatgtc atgcgcgagc atggctatcg cgttcacctg gctcacgatg tgcctgacgc
     4381 cgaacaggcc attcagcggc atgcttttca ggtcgtgctc gtcgatatga ggcttccttc
     4441 tggagatggc gggcaggtcc ttcggctgct gcggcaaaag gaccaatccg ccagggcctt
     4501 gatcatcacc ggcttcgcca gcgaaatgga gaggcgggtc caagaggctt tggccgcggg
     4561 cgccactgca gtttgttaca agcctttcga tgtcgaaaag ctgctgacaa cggtccacga
     4621 cttgtgcaaa cctccggcga attgacttag gccagcttgc atggagaaca gtgctacgac
     4681 gaattccgcg ttcagtgagc tggtgctcgt catcgatgac gatgccgaca cgcgcgagaa
     4741 cctgtgcgac atcctggagc tggacggcta tcgctatgag gcggctggaa ccgccagcga
     4801 attgttagag cggtccaact gggatgacgt tgcacttgtc cttttggacc gcaaactacc
     4861 tgacggttcg cctcaggaga ttttgcctcg cctgaaggtg ttggcgccgc aggtggctgt
     4921 catcatcgtc accggctatg cggatatcga gggagcaatc tctgccctcc agagcggagc
     4981 tgcagattac attctcaaac cgatcaatcc cgatgcgctg cgagcgagcg tccggcgggt
     5041 catacagcga cagcgagatc tgcagcagat tgcttgcctt agcagcgatc tggaagagag
     5101 cgaggttcgt tatcgcgcac ttttcgagaa cacgatggat ggcctggtga ttctggacga
     5161 tgagagtcgg atttttgccg ccaatccagc cgcgagatcc atgttgggat ggaccggtgg
     5221 ggcaagcgag atgaagctag agtcgctgaa gctggacaat gaggggcaaa tgaacgccgc
     5281 cacgcgcggc gattttttgg gaccgggcaa gcacagtggt gagtactgga tcgccgtgcc
     5341 ggggggcaga atcgctatcg aataccgggc cgtcgagaat tccgctcccg gcttgcatct
     5401 gatctccatg cgtgatgtaa cggcccgcaa atgtgcggag gagcgtgccc gccaggccga
     5461 acgattggct gccatcgggg aaacgatgac cgctctggtc catgagagcc gcaatgcgct
     5521 gcagcgaagc aaagcctcac tggagttgct gaagctcgaa gtagaagacc gtcccgaggc
     5581 gaccaaactg gcaaaccgtg tcgagcgcgc tcaggaagac ctgcaccagc tctttgagga
     5641 ggtgcgccag tgggcggcgc ccctcaagct gcgccgcgaa gtgtgcaact tgcgcgagtt
     5701 atggcaagag acctggtgcc ttgtcggcca ggccaatccc gctcgcaagg ccaaactcgt
     5761 cgaagagttg acctgtgaac ccatttgcca tgccgacgaa ttcgccttgg gccaggtttt
     5821 tcgaaacatc tttgagaacg caattgaagt cgcaccgccc ggcagcaatg tgacaatccg
     5881 atgcgcgcac ccgcccagcg gcaatggcaa tgtgatcgtt ctgacgatac gcgacgaggg
     5941 cccaggactc actgccgagc agcagcagcg catttttgag ccgttcttca ccaccaaagc
     6001 caagggaact ggctt
//
LOCUS       DASUGB010000139         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1266348, whole genome shotgun sequence.
ACCESSION   DASUGB010000139 DASUGB010000000
VERSION     DASUGB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266348"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFV87_02745"
     CDS             <1..672
                     /locus_tag="VFV87_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102698.1"
                     /translation="WYQYLCRDQLLVWLPACFIGIALPSMLSVEFLPRGTEAGDWNSA
                     VMTADGVRQHVTNPPSGVLVTDAGLAPYLAGPGWGKVFWSLTLLCGFLVLGTTLVSTI
                     DGIVRRWVDVFWTASPRLRRVDPHHIKYVYFGVLIAYSVFGITMLWLNKPAELIKWAT
                     LGYNFALGFSCWHTLALNRILLPPKLRPSLVPAIGLVLGGIFFWTLGTVAVLDQFSKL
                     GWIHL"
     gene            729..1232
                     /locus_tag="VFV87_02750"
     CDS             729..1232
                     /locus_tag="VFV87_02750"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX5102699.1"
                     /translation="MLQPQIVEKPALTVVGLEASFLHALSPQSTTFKVIPALWESFLH
                     RAKEVPARLGAASYGIIYGRPEAQRSHPHELQYIAGVAVRSTDNVPAGMVAHTVAPGT
                     FAVFLHRGSIAKIGDTCREIYRVWLPQSAWEHAGIADVELYDERFAGQSDRSEMEYWV
                     SVMPKMP"
     gene            1613..1825
                     /locus_tag="VFV87_02755"
     CDS             1613..1825
                     /locus_tag="VFV87_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102700.1"
                     /translation="MIDARKSVRQGREVPSNLIIWIVIALGIGGCMYWQNQAHRRYLE
                     TTSPEQQAIDRDEMVRHDAFDDERFP"
BASE COUNT          372 a          623 c          594 g          413 t
ORIGIN      
        1 tggtaccagt acctctgccg cgaccaactg ctggtctggc tgccggcctg cttcatagga
       61 atcgcgctgc cgagcatgct ctcggtcgag ttcctccccc gcggcaccga ggcgggggac
      121 tggaactccg ccgtcatgac ggccgacggg gtccgccagc acgtcaccaa tccgcccagc
      181 ggcgtgctgg tgaccgacgc cggcctcgcg ccgtatctgg ccggccccgg ctggggaaag
      241 gtcttctggt cgctcacgct cttgtgcggg tttctcgtct tgggaacgac gctcgtctcg
      301 accatcgacg gcatcgtccg ccgctgggtc gatgtcttct ggaccgccag cccgcggctg
      361 cggcgggtcg atccgcacca catcaagtac gtctatttcg gcgtgctgat cgcgtattcc
      421 gtgttcggca tcaccatgct ctggctcaac aagccggcgg agctcatcaa gtgggccacg
      481 ctgggctaca acttcgccct gggctttagc tgctggcaca cgctggcgct caaccgcatc
      541 cttttgccac ccaagctgcg gccgagcctg gtcccggcga ttggcctggt gctcggcggc
      601 attttcttct ggacgctggg gaccgtagcg gtgctggacc agttttcgaa gctgggttgg
      661 atccatctgt aagggcattt cgccgcggtg ctcgcaccgc gctcgctgca gttaccacga
      721 ggcaaaccat gctccagccg caaattgtcg aaaaaccggc cctgacggtc gttggcctgg
      781 aggcgtcgtt cctgcacgct ctctcgccgc agagcacgac cttcaaggtg attccggccc
      841 tctgggagag cttcctgcac cgtgccaagg aagtgccggc ccggcttggc gcagccagct
      901 acggcatcat ctatggccgc ccggaagccc agcgctcgca cccccacgag ctgcaataca
      961 tcgccggagt cgccgtccgt tcgacggaca atgtcccggc cggcatggtc gcgcacacgg
     1021 ttgctcccgg cacgttcgcc gtcttcctcc accgcggctc gattgccaag attggcgaca
     1081 cgtgccgcga gatttatcgc gtctggctgc cgcaatcggc ctgggagcac gcgggcatcg
     1141 ccgacgtgga actctacgac gagcgcttcg ccggccaaag cgaccgctcg gagatggaat
     1201 attgggtttc ggtgatgccg aagatgccat agtcggcaaa acctccgcgc tcgatggggc
     1261 cggctcgcgc ttgccgacaa tgttttacag tgtaaaatca tccactgcct gccgatgctc
     1321 agttttgcta tcagtactcg gggatataga acatggaaat tcgggaactc cgacaacctg
     1381 gtaagtggct tctccgaact cgatctggac gtggcctagt aatggagtgg atacaagttg
     1441 tcgcagtttc gccaggagcg cctattgcat caggccagct tcttgaacgg tatccaacga
     1501 ttgatggctc acgcgtactg catactcgcg atcgcgtctt tctcgtgcct tctgaggacg
     1561 gtgcagagga agcctttctc gttggtttct ggcacccccc atctggaacg ctatgattga
     1621 cgcccgcaaa tcagtccggc agggacgcga ggtaccctcc aatttgatta tctggatcgt
     1681 gatcgcactt ggaatcggag gatgcatgta ctggcaaaat caagcacatc ggcgttatct
     1741 ggaaaccaca agtcccgaac agcaggcaat agatcgggac gagatggttc gccacgatgc
     1801 attcgatgat gaacgatttc cctgacgggc tatgtccgca ctcaacgcgc aaaagaagag
     1861 gccttcatga cgccggtcga gcgacgttac agcaaagagg aatttgcccg ccggggcgat
     1921 gaaatctacg aaagcgagat tgcgcctcgc ctcaaggcga gcgataaggg caagttcctg
     1981 gcaatcgata ttgattcgcg cg
//
LOCUS       DASUGB010000140         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_294192, whole genome shotgun sequence.
ACCESSION   DASUGB010000140 DASUGB010000000
VERSION     DASUGB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294192"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFV87_02760"
     CDS             complement(<1..302)
                     /locus_tag="VFV87_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102701.1"
                     /translation="MRANLRFVLYLTALGPLAAVADEKPPTGPETEKRFPPLVVPAGF
                     QATLFACDPLIEYPSGIAAGPKAGTAFVAIDYMTGLGTKIVRRSEVRLVQDTDGDG"
     gene            459..773
                     /locus_tag="VFV87_02765"
     CDS             459..773
                     /locus_tag="VFV87_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102702.1"
                     /translation="MAKCDVCGNDYDKSFTVTMGGKMHVFDSFECAIHMLAPVCQHCG
                     CKVIGHGVEKDNAIFCCAHCAEQGGVHGLKDRISKVAGDYNPVFPHDPNLPVSQPTMV
                     PP"
     gene            complement(788..1801)
                     /locus_tag="VFV87_02770"
     CDS             complement(788..1801)
                     /locus_tag="VFV87_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5102703.1"
                     /translation="MKTAAFFAALMTVSLLASAARAEDLPQLKIGIIGLDTSHVIAFT
                     KELNSPNAAPELAGCRVVAAYPKGSSDIESSVSRVPGYVEQITKLDVEIVESIDELLK
                     RVDCVLLETNDGRPHLEQALPCFKAGKPTFIDKPLAGSLSDCIAIFEAAKKYNCPTFS
                     SSSLRYGKDTQAVRGGSLGKISQCQTSSPASLEKTHPDLFWYGIHGVEALFTAMGPGC
                     ESVTRGKTANGLIEVVGTWEGDRTGKFVESKGYGGTAKGEKGEASVGSSDGYRPLIVE
                     IVKFFRSKQAPIAEAETLEIYAFMEAADESKRQGGAAVKLDGVLATARQQAAKRLAEL
                     GVK"
     gene            complement(2175..2456)
                     /locus_tag="VFV87_02775"
     CDS             complement(2175..2456)
                     /locus_tag="VFV87_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102704.1"
                     /translation="MKRLLIICVTGLALMLGGLIPAENADARPWGWGGYYRPGVSVYA
                     GPRYGYRSYYRPYAWRPYYRYYGYPNYYSYPRYSRGFYYYGPYAGVYVR"
     gene            2809..3225
                     /locus_tag="VFV87_02780"
     CDS             2809..3225
                     /locus_tag="VFV87_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102705.1"
                     /translation="MYALLAAAAIGLLPLDFVTTDTADVIELNHFYDGDGRPVFDQVI
                     FWQWHEREGAYHVLAWRLWKTPAQSPWRDWLRGGYAMAWFDGERLRVVRATSFTETWT
                     QHDPELDDRQYFPVNLRRGLIGERAGPPAQAPSTQP"
     gene            3320..4726
                     /locus_tag="VFV87_02785"
     CDS             3320..4726
                     /locus_tag="VFV87_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102706.1"
                     /translation="MSSLRPLVVLILLACAAPAFADERRAETAGRNWPAWRGPLATGE
                     APLADPPIEWDSDGTNIRWKAAIPGRGHSTPIVWGNRIFLTTAVPVGEALPPRPSTAP
                     GNHDNLPVTHRQKFVALALNRIDGKIVWQKSLREALPVEQGHRTGSLASSSPVTDGEH
                     LIVSFGSFGVYCLDFDGNLIWKKDFGRMDTLHGHGEGASPVLHGNTVVVNWDHEGESF
                     VAALDKRTGKLIWRADRDEVTSWATPIVVTQTRRASEGTTVTRSVSEEATSTRSVSED
                     AQLIVPGTARIRGYDLTTGKVLWECGGLSSNIVASPVHADGMLFAGSSYDKRALLAIR
                     LEGAAGDITGTDHVVWSRFRGTPYVPSPLLVDGGLYFLTHYQPIITRLDAPTGEDKPG
                     AIRLEGLGNIYASPVSAGGRVYITDLDGTTLVMTTGEIPRALALNPLGEAVSASAALA
                     GNELFLRGEKHLYCIAGD"
     gene            complement(4762..5163)
                     /locus_tag="VFV87_02790"
     CDS             complement(4762..5163)
                     /locus_tag="VFV87_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5102707.1"
                     /translation="MARSLLTLIVLPIFALVAYAVEPLAHTKDSLDTVKENLQAGKAV
                     MVDVREQSEWDEAHLKIAIHLAKSKLEAAADIEALLKNLPKDKVIYTHCKAGGRALAC
                     GKLLKEHGYDVRPLKPGIQALLDAGLEKAEK"
     gene            complement(5227..>5842)
                     /locus_tag="VFV87_02795"
     CDS             complement(5227..>5842)
                     /locus_tag="VFV87_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102708.1"
                     /translation="DAGRKLLPKPRAIECVIIGDTFSGDELIFHPCRPDALFVLPSEC
                     GRVFDAGSDLLTAVDWMCSSGKLTSRISKLVFEPFDSRLGSDDQDSGNEEVVDPPGES
                     LDALIELAKYWAERHRISKLARDDIKSAIEKHQKVEMLFSAISFSGHDPHGPGCIAQF
                     QVTDKASGLDLGTLYWNKDDVSHGFEIEWNETNVAKARRSRRGR"
BASE COUNT         1085 a         1868 c         1767 g         1122 t
ORIGIN      
        1 tatccgtcgc cgtccgtatc ctgcaccagc cgcacttcgc tccgacggac aatcttggtg
       61 cctaaacccg tcatgtagtc gatcgccacg aacgccgttc cggccttcgg cccggccgcg
      121 atgcccgacg gatactcaat cagcggatcg caggcaaaga gtgtcgcctg gaaaccggcc
      181 ggcacgacca gcggcgggaa acgtttctcg gtttccggcc cggttggcgg tttctcatcc
      241 gccacggctg caagcgggcc gagggcggtc aagtagagca caaaccgtag attggcgcgc
      301 atgactctct ccgccacaag aaatggactg gttgtcagtc tatcgacccc gcggcggtgt
      361 ctcaagaaat cggctggctt gtggcccaag caactcgttc tggcatttcg tttgcaagcg
      421 aaaagccgcg ctgcctaact tctgcaagga gattcgccat ggccaaatgc gatgtttgcg
      481 gcaacgacta tgacaagtca ttcacagtga cgatgggggg gaagatgcac gtgttcgact
      541 cattcgagtg tgcgatccac atgctcgccc cggtttgcca gcactgcggc tgcaaggtga
      601 tcggtcacgg cgtggagaag gacaacgcca tcttctgctg tgcccactgt gccgagcaag
      661 gcggcgttca cggcctgaaa gatcgcatct cgaaagtcgc gggcgactac aaccccgtct
      721 tcccacacga tccgaacttg cccgtttccc agccgacgat ggtgccgcca taaattggct
      781 gtgagcacta tttcacgccc agctccgcca gtcgcttcgc ggcttgctgg cgagcggtag
      841 cgaggacgcc gtcgagcttg accgccgcgc cgccttggcg cttgctttcg tcggcggctt
      901 ccatgaaggc gtagatttcg agcgtttcgg cttcggcgat cggcgcctgt ttggagcgaa
      961 aaaacttgac gatttcgacg atcagcgggc gatagccgtc ggagctgccg acagacgctt
     1021 cgcccttctc gcccttggct gtgccaccat agcccttgct ctcgacaaac ttgccggtac
     1081 ggtctccttc ccaggtgccg acgacctcga tcaggccgtt cgccgtcttg ccgcgcgtta
     1141 ccgactcgca tcccggcccc atcgccgtga acagggcctc gacgccgtga atgccgtacc
     1201 agaacaggtc gggatgggtc ttttccagcg aagccgggct ggaagtctgg cactggctga
     1261 tcttgcccag cgatccgccg cggaccgcct gggtgtcctt gccgtaccgc agcgacgacg
     1321 acgagaacgt cgggcagttg tatttcttcg cggcctcgaa gatcgcgatg caatctgaca
     1381 gcgagcccgc cagcggctta tcgatgaagg tcggcttgcc ggccttgaag caggggaggg
     1441 cctgctccag gtgcggccgg ccgtcgttcg tctccagcag cacgcaatcg acccgcttca
     1501 gcaattcgtc gatcgattcg acgatctcca cgtccagctt cgtgatctgc tccacgtatc
     1561 ccggcacgcg gctgacgctc gactcgatat ccgagcttcc cttcggatac gccgccacca
     1621 cgcggcagcc agccagctcc ggcgcggcat tggggctgtt cagctccttc gtgaaggcga
     1681 tcacgtgcga cgtatcgagc ccgataatgc cgatcttgag ctgcggcagg tcctcggcgc
     1741 gggcggccga tgccaggagg gagactgtca tgagggcggc gaagaaggcg gcggttttca
     1801 tgggatgtga ggcaggcatc atgcctgcca ggaaggaagt ttggcgcggc gaggatattg
     1861 cgcgagtggc tggttagctt tggcaggccg gaagcctgcg ccacgtcggc gaggattaat
     1921 ttcgctgctc cgccggcaac ttgcaaggaa tccggcccga gaatttaccg ccccgccact
     1981 tgccgcgggg cggcggccct gagagctgta ttcaccgcgg gcggccttct ttgccgtgcc
     2041 gcccgggacg atcagcctca ccgacaccac gccgcatcag acgaccagcg gcgcttttcg
     2101 ctgcttcgca ggcgcttggc gcccgctccg caccaaggta cttgcgcgag gccccagccg
     2161 atcattgttg ctcactaccg aacatacaca cccgcatacg gcccgtaata gtagaaccca
     2221 cggctatagc gcgggtaact gtagtaattc ggatagccgt aatagcggta gtacggtcgc
     2281 caagcgtagg gccggtagta ggaccggtat ccataccgcg gtccggcgta aacgctaacg
     2341 cccggccggt aatagccgcc ccagccccac gggcgagcat cggcgttttc agcagggatc
     2401 aagccgccga gcatcaaggc cagaccggtc acacaaatga taagtagccg tttcatggct
     2461 ctcctccttt ttctttcgta cctgagatat gtcgatgatc gcacgatcgc tcgacaaagg
     2521 agaaggaagc agcgcgcgtg ccgagcgtga aggaattgcg gcgctcgtcg cgggatttaa
     2581 gagcggcatt tccccccggc agcgggtttc ctttgacgcc ttggctgcaa taattcgagc
     2641 agacgcgtgg cgaaacagcc ttttcggtga gccgccgcga atgaacgtaa tcaactcgtc
     2701 acgaacgatc cccgcagccc gctgggcagc aatcgccagg ggcagcgctc ggctcgccga
     2761 ttcggcacag gcgggagctt ggagcttccc gcgagggctc gaagaatcgt gtacgcactc
     2821 cttgccgcgg cggccatcgg tttgctgccg ctcgattttg tcaccaccga tacagccgac
     2881 gtcatcgagc tgaaccactt ctatgacggc gacggccggc cggtcttcga ccaggtaatc
     2941 ttctggcaat ggcacgagcg ggagggcgcc tatcacgtgc tggcctggcg gctctggaaa
     3001 acgccggccc agtcgccgtg gcgcgattgg cttcgaggcg gctacgccat ggcctggttc
     3061 gatggcgagc ggctgcgggt cgtgcgggcc acgtcgttca ccgagacttg gacacagcac
     3121 gatccagaat tggacgaccg ccagtatttt cctgtgaatt tgcggcgggg gctgatcggt
     3181 gaacgagccg ggccgcctgc ccaggcgccg agtacacagc cgtaggacgg gtttccaact
     3241 tgaccagcgg ccagccaggc aattcgaccg aacgaacagc cgtcccaccg cgtatagtgt
     3301 tggcaaccgg gagtctccca tgtccagctt gcgcccgctc gtggtgttga ttctgttggc
     3361 ctgtgccgcg ccagcattcg cggacgagcg tcgcgcggaa actgccggga gaaactggcc
     3421 agcctggcgc ggaccgctgg cgacgggcga agctccgctg gccgatccgc cgatcgagtg
     3481 ggattccgat ggcaccaaca tccgctggaa agcagcgatt cccggccgcg ggcattcgac
     3541 gcctatcgtc tggggcaatc gcatcttctt gacgaccgcc gttcctgttg gcgaggctct
     3601 gccgccaaga cccagcaccg ccccgggcaa tcacgacaac ttgcccgtca cgcaccgtca
     3661 gaagttcgtc gccctggccc tcaaccgcat cgacgggaag atcgtctggc agaagtcgct
     3721 ccgcgaggcc ctgccggtcg agcaaggcca ccgcaccggc agccttgcgt cgagttcgcc
     3781 cgtcaccgac ggcgagcacc tgatcgtgag cttcggatca tttggcgtat attgccttga
     3841 tttcgacggc aatctgatct ggaagaaaga cttcggccgg atggacacgc tgcacggcca
     3901 cggcgaaggg gcttcgcccg tcctgcacgg caacacggtc gtcgtcaact gggatcacga
     3961 gggggagtcg ttcgttgcgg cactcgacaa gcgcaccggc aagctgatct ggcgcgccga
     4021 ccgcgacgaa gtcacaagct gggccacgcc gattgttgtc acacaaaccc gacgcgcaag
     4081 cgagggaacc acagtaaccc gaagtgtcag cgaggaagcc acatcaaccc gaagcgtcag
     4141 cgaggacgcg cagctcatcg tccccggcac cgcccgcatc cgcggctacg acctgaccac
     4201 cggcaaggtc ctttgggaat gcggcggcct gtcgtcaaac atcgtcgcct cgcccgtcca
     4261 tgccgacggg atgctcttcg ccggcagcag ctacgacaag cgggccctgc tggccatccg
     4321 attggaaggc gcggcgggcg atatcaccgg caccgaccac gttgtctgga gccgtttccg
     4381 cggtacgccc tacgtgcctt caccgctatt ggtcgatggc ggactctact tcctcacgca
     4441 ctatcagccg attatcacgc gcctcgacgc cccgaccggc gaagacaagc ccggtgcgat
     4501 ccgcctggaa gggctcggca acatctatgc ctcgcccgtc tccgccggcg gtcgcgttta
     4561 tatcaccgac ctcgacggca cgacgctcgt catgaccacc ggcgaaatcc cccgcgctct
     4621 ggccctcaac cccctcggcg aggccgtttc ggcatctgcg gcattggctg gcaacgagct
     4681 attcctgcgc ggtgagaagc atctgtattg catcgctggc gattgatcct gtcgctgcag
     4741 aatagcaaag gcgacgccgg cctacttttc ggccttctcc agtcctgcgt ccagcagcgc
     4801 ctgaatgccc ggcttgaggg gccgcacgtc gtagccgtgc tccttgagga gtttgccgca
     4861 ggccagcgcg cggccgccgg ccttgcagtg cgtatagatc accttgtcct tgggcaggtt
     4921 cttgagcagc gcttcaatat ccgcagccgc ctcgagcttg ctcttggcca gatgaatcgc
     4981 gatcttcaaa tgcgcctcgt cccactcgct ctgctcgcgg acatcgacca tcaccgcctt
     5041 gcctgcctgc aggttctctt tgacggtgtc gagcgagtct ttggtgtgcg cgagcggctc
     5101 gaccgcgtag gcgaccagcg caaagatagg tagcacaatg agtgtgagga gggatcgggc
     5161 catgacgaat tctccgttgg ggtttgaggt gtggaggaag gataaggcag gaaagaattc
     5221 tgcttgctat cgcccgcggc gcgacctgcg tgccttggcg acattggttt cgttccactc
     5281 tatttcgaag ccgtgcgaca catcgtcctt attccaatag agcgtaccaa ggtcgagtcc
     5341 acttgccttg tctgtgactt ggaattgggc aatgcaaccg ggaccatgcg gatcatggcc
     5401 gctgaaggag atcgccgaga acagcatttc taccttttgg tgcttctcga ttgcgctttt
     5461 gatgtcgtcg cgtgccagct tagaaatgcg gtggcgctcg gcccagtact tcgcgagttc
     5521 aatgagcgcg tcgagcgatt cccccggtgg atccacaacc tcttcgttgc ctgaatcctg
     5581 atcgtccgat cccaatcgac tgtcaaacgg ctcgaaaacg agcttggaaa tacgggaagt
     5641 cagttttccc gacgagcaca tccaatccac agccgtcagc aaatcggagc cggcatcgaa
     5701 gacccttccg cattcgctcg gcaggacgaa cagcgcatcg ggacggcagg gatgaaaaat
     5761 caactcgtcc ccgctgaacg tgtcgccaat gatcacacat tcgatcgctc ttggcttagg
     5821 aagaagcttg cggcccgcgt cc
//
LOCUS       DASUGB010000141         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_711000, whole genome shotgun sequence.
ACCESSION   DASUGB010000141 DASUGB010000000
VERSION     DASUGB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711000"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFV87_02800"
     CDS             <1..259
                     /locus_tag="VFV87_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102709.1"
                     /translation="SFLVGTLVILVVLPIIVIGSAALVAQGNVRMWPWIAIASGLIPL
                     GTGCVCGNLVFAFWLLHLMLRKDIRAALASPEIALESKRTR"
     gene            complement(278..>2562)
                     /locus_tag="VFV87_02805"
     CDS             complement(278..>2562)
                     /locus_tag="VFV87_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5102710.1"
                     /translation="ERGLKFARRKPAWAGLGLVIALSTVALAVTTATYTRSLSRTNAE
                     LDSSNTKLQETNTDLRRTNYSALIDKTEDYWSRDPRNLARGMALLNSLVPAEGEADLR
                     GIEWYFLRRLCDVQQQELYQAAGQAADLCCREDGSLLIHDPQREESLISLAGGAATSR
                     TALVRGSNVVRWQFAPNSPWCLAWTDDYKLELRSSANPDDPGEEIARNEPVDIPASFS
                     RDGSLVALIPRGQPPALWRREEPGRFRAITTSIARPLDVKLSPDGKWAATRDESQFAV
                     TKTDTRKTNLIETTHGQRKYTAMAFSPDSQRLALASRLGQLDIVLLEEKLTQISLNLK
                     RDFATPKALEFLPGGRRLALASSNAQVFVFDLRHDVVSMDDQDTAVYRGHRAGVEHLA
                     ADPSGKFLYSSAQDGQVLRWDLSVKDQRTHDIIEQTGMWFMNFNCLSYSPDGRWLAAG
                     GVSYVNGRDNQSGLLLLIDVTTRQVRHRLDTPQPITSCSFSASGKRLLYRAAEADASN
                     SQDKSQIHVWNVEAEKPIGDVDALLAADAACLAGDGRVVFAGAVGGLRLLDLESGTSN
                     EWGELPGRPPVRIESLVASAGGTLIAGRSAGGVLRLWDATSGRQRLQSVGHGPAAVMA
                     LSADATLAAWTTPEFVSSDKAGEAGQKSSPEAEIAITSPREQQALVFDLAANRIRFAI
                     GGHSSPIQTLAISADGKRLASGDAEGILKLWDTTSGQELMAIEDAHTGKALAAAFHPN
                     NREMATIGSRDGKVRIWWTE"
BASE COUNT          441 a          822 c          785 g          514 t
ORIGIN      
        1 gtcgttcctg gtcggaacgc tggttattct ggtagtactg ccgatcatcg tgatcgggtc
       61 agcggcgctt gtcgctcagg gcaacgttcg catgtggccc tggattgcaa tcgcaagcgg
      121 cctcattcct ctgggaacag gctgtgtgtg cggcaatctt gtcttcgcct tctggctcct
      181 gcaccttatg ctccgcaagg acatccgcgc cgcgctagca tctcccgaga tagctttgga
      241 gtcaaaaaga acccgctagc caccggcgct cctccgctca ctccgtccac cagattcgca
      301 cttttccgtc tcggctgccg atggtcgcca tttcccgatt gttcggatga aacgcggcgg
      361 ccagtgcctt gcccgtgtga gcgtcctcga tggccattag ctcctggccg gacgtcgtgt
      421 cccacagctt gaggatgcct tccgcgtcgc ccgaagccag ccgtttgccg tcggccgaga
      481 tggcgagcgt ctgaatcggc gagctatggc cgccgattgc gaagcgaatt cggttcgccg
      541 ccaggtcgaa cacgagtgct tgttgctcgc gcggcgaggt gatcgcgatt tcggcttccg
      601 ggctggactt ctggcccgcc tcgcctgcct tgtccgagct gacgaattcc ggcgtcgtcc
      661 aagccgcgag cgtggcatcg gcagaaaggg ccatgacggc cgcgggaccg tgccccacgg
      721 actgcagacg ctgccggcca cttgtcgcat cccacagccg cagcacgcca ccggcgctgc
      781 gcccggcaat cagcgttccg ccggcagatg cgaccagcga ttcgatacgg accggcggcc
      841 ggccaggcag ctccccccat tcgttgcttg tgccgctctc aagatccaga agccgcaatc
      901 cgccgactgc cccggcaaac acgactcgcc cgtctccagc caggcacgcc gcgtcggcag
      961 caaggagggc atcgacgtcg ccaatgggtt tctcggcctc cacgttccaa acgtggattt
     1021 gcgatttgtc ttgggagttg gaggcatcgg cttccgcggc gcgatagagc aaccgcttgc
     1081 cgctggcgga aaacgaacac gaggtgatcg gctgcggtgt atcgagccga tgacggacct
     1141 ggcgcgtcgt cacgtcaatg agcagcagga gaccgctctg attgtctctg ccattcacat
     1201 acgatacgcc tcctgccgcc agccagcggc cgtccggcga atagctcagg caattgaagt
     1261 tcatgaacca catgccggtc tgctcgatga tgtcgtgtgt ccgttggtct ttgaccgaca
     1321 aatcccaccg caggacctgg ccgtcttggg cgctgctgta gaggaacttc ccagacggat
     1381 cggcggcgag gtgctcgacg cccgcacgat ggccgcgata cacagccgtg tcctgatcgt
     1441 ccatgctcac gacatcgtgc ctcaggtcga acacaaagac ctgcgcattg gacgaggcca
     1501 gcgccaaacg ccggccgcct ggcaaaaact ccagcgcttt gggagtggcg aaatcgcgct
     1561 tgaggttgag cgaaatctga gttagctttt cttccagcag cacgatatcc agttgtccca
     1621 gccgcgaagc cagcgccagt cgctggctgt cgggcgaaaa tgccattgcg gtatatttgc
     1681 gttggccgtg cgtggtttcg atcaggttgg tcttgcgcgt atcggttttt gtgaccgcga
     1741 attgtgactc gtcccttgtc gcggcccact tgccatcggg cgaaagcttg acgtccagcg
     1801 gccgggcaat cgacgtggtg atcgctcgaa atcgtcccgg ctcctcgcgc cgccacagcg
     1861 ccggcggctg tccgcgaggg atcagcgcga cgagactgcc gtctcgagag aaagaagccg
     1921 gaatatcgac gggctcgttg cgtgcgattt cctcgcctgg atcgtctggg ttggcgctgg
     1981 aacgcaattc cagcttgtag tcgtccgtcc aggccaggca ccaggggctg tttggggcga
     2041 attgccagcg cacgacgttc gatccgcgga cgagcgccgt ccggctggtg gccgcgccgc
     2101 ccgccagcga gatcaaactc tcctcgcgct gcggatcgtg aatgagcagg ctcccgtcct
     2161 cgcgacagca gagatctgcg gcctggcccg cagcctgata taactcctgc tgttgcacgt
     2221 cgcagagccg gcgcaggaaa taccattcga tgccacgcaa atccgcctct ccctcggccg
     2281 ggaccagcga attgagcagg gccatgcctc gcgccagatt ccgcggatcg cggctccaat
     2341 aatcttcggt cttgtcgatc agggcggaat aattggtccg gcgcagatcc gtgtttgtct
     2401 cttgcagctt cgtattgctc gaatccaact cggcgttcgt cctagaaagg ctgcgagtat
     2461 aggtggcagt ggtgacggcc aatgccacag tcgatagagc gatcaccagc ccgagccccg
     2521 cccaggccgg ttttcgtcgg gcgaatttga gcccgcgttc gg
//
LOCUS       DASUGB010000142         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_294474, whole genome shotgun sequence.
ACCESSION   DASUGB010000142 DASUGB010000000
VERSION     DASUGB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294474"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFV87_02810"
     CDS             <1..1008
                     /locus_tag="VFV87_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEX5102711.1"
                     /translation="LDTLQWGTAASATTNALGAYSLAALPAGDYHVRVNPPPNHTVTT
                     YPTGIATLTLGIAQALTGINFGIAFGANPWHNFASNLNVNADPDGVISPVDALMVINW
                     LNSHSTAQLPGQATPQIHGFVDVNNDSLCTPIDALLVINWLNSEQNVPPPAEGEGSAP
                     AFAPAGNLPAAGNSPAEGETSPQTVAEYFAKNPLHFLEIAGDDDLCVHDEHSDLGPTF
                     QAAASQVVPALASTGPISRASSLSGAPLLLAGGSSEIGQRLSTIGQELHDRLEQQLGD
                     SLPRHTLQPVLDRVQGAKETLAVVGDELDEALEAIAPDVSHQWQAALERILRRFPHGL
                     G"
     gene            complement(1065..>2354)
                     /locus_tag="VFV87_02815"
     CDS             complement(1065..>2354)
                     /locus_tag="VFV87_02815"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5102712.1"
                     /translation="QVAGGLKPGWIYEVVYEAEGSVVQGVGLAGIRDAVSILKYERGE
                     RNPLCDASGKPLIARAIGFGTSQSGRCLRHFLWEGFNADEEGRQVFDGLIPHVAGGGL
                     GSFNHRFAAPTRTNGQHEEHLFPADYFPFTYGETKHPLTGKADSILARSRATKTVPKV
                     MHTQSSSEYWHRSGSLVHTDPLGIRDKEIPPEVRIYTFGGTQHGSGGGVPGPAGSGQL
                     PNTPADYKPLLRALIIALDEWIKDGKEPPPSVFPKIRDKTLAGWEQRHSGWPAIPGVT
                     YPTVIHEPKVLDRGPDWEAKRIATREPPKIMFHFHVRIPAIGPDGNELGTLLHPAVAV
                     PIGSYTSWNLRSEAIGAPGELLGLAGGYIPFAKTAADRKASGDPRPALLERYKDFADY
                     RAQYQAAAEKLVAERYLLPEDLPRAMALCEKFRPAFE"
BASE COUNT          406 a          766 c          744 g          438 t
ORIGIN      
        1 ctggatacgc tgcagtgggg cacggcggcc tcggccacaa ccaatgcact gggggcctat
       61 tcgctcgccg cattgccggc cggcgattat cacgtccgcg tcaatccgcc ccccaatcac
      121 acggtcacga cgtatccgac gggtattgcc acactgaccc tcggcatcgc tcaggcactg
      181 actggcatca actttggcat cgcgttcggg gccaacccgt ggcacaactt cgcgtcgaac
      241 cttaacgtca acgcggaccc ggacggcgtc atctcgcccg ttgacgccct gatggtgatc
      301 aattggctca attcgcattc caccgcacag cttcccggcc aggcgacgcc gcagattcat
      361 ggatttgtgg atgtgaacaa cgatagcctg tgtacgccaa ttgatgcttt gctcgtgatc
      421 aactggctga attcggagca gaacgtgccg ccacccgccg agggcgaggg gagcgcgcca
      481 gcgtttgcac cggcgggaaa tctgcccgct gcggggaatt cgcctgccga gggagaaact
      541 tctccgcaaa cggtcgcgga atacttcgcc aaaaatccgc tgcacttcct cgagattgcc
      601 ggcgacgacg acctatgtgt tcacgatgag cacagcgact tgggcccgac gttccaggct
      661 gccgcatcgc aagtcgtacc ggcacttgcc tccaccgggc cgatctcccg cgcttcttcg
      721 ctgagcggcg cacccctcct gctcgccggc ggatcgagcg agatcgggca gcgactctcc
      781 actatcggcc aggagctgca cgaccggctc gagcagcagc tcggcgattc gttgccacgc
      841 catacgttgc agccggtttt agatcgcgtc cagggcgcca aggagacgct ggcggtggtg
      901 ggcgacgagc tggacgaagc gctggaagcg atcgctcccg acgtgtcgca ccagtggcag
      961 gcggctctcg agcggattct ccggcgcttc ccgcacggtc tgggctagcc tcagtaaccc
     1021 gaagcgtaag cgagggaatc tctgcagcgt cgtaagcgag ggaatcactc aaacgcaggc
     1081 cggaactttt cgcacagggc catcgcccgc ggcaggtcct ccggcaagag gtatctttcg
     1141 gccacgagct tctccgccgc ggcttgatac tgggcgcggt agtcggcgaa gtccttgtag
     1201 cgctcgagca gcgccggccg cggatcgccg ctggccttgc ggtctgcggc agtcttggcg
     1261 aaagggatgt agccgccggc caggccgagc aattcgcccg gcgcgccgat cgcttcgctc
     1321 cgcaagttcc agcttgtata gctgccgatc ggcacggcga ccgcgggatg caatagcgtg
     1381 cccagctcat ttccgtctgg gccgatcgcg ggaatcctga cgtgaaagtg gaacatgatt
     1441 ttcggcggct cgcgggtcgc gatccgcttt gcctcccagt cgggaccgcg gtcgaggact
     1501 ttcggctcgt gaatgacggt cgggtatgtc actccgggaa tcgccggcca gccgctatgc
     1561 ctttgctccc agccggcgag cgtcttgtcg cggatcttgg gaaagacgct ggggggcggc
     1621 tctttgccgt ctttgatcca ctcgtccagg gcgatgatca gcgcccttag cagcggcttg
     1681 tagtcggcgg gcgtattggg caattggccg ctgccggccg gaccgggaac gccgccgccg
     1741 ctgccgtgct gcgtgccgcc gaaggtgtaa atgcgcacct cgggtgggat ctccttgtcg
     1801 cggattccca gagggtcggt gtgcacgagc gatccgctcc gatgccagta ttcgctcgaa
     1861 gactgagtgt gcatcacttt gggaacggtc ttcgtggccc gcgagcgagc cagaatgcta
     1921 tccgccttgc cggtcagcgg atgcttcgtt tcaccgtaag tgaacgggaa gtagtcggcg
     1981 ggaaagagat gctcctcgtg ctgaccgttg gtccgcgtgg gcgcggcgaa acggtggttg
     2041 aacgaaccca ggccgccgcc ggcgacgtgc gggatcaggc cgtcaaagac ctgcctgccc
     2101 tcctcgtccg cgttgaagcc ttcccacaaa aagtgccgca ggcaccggcc gctttgcgaa
     2161 gtgccaaacc cgatcgcccg cgcaattagc ggcttgccgc tcgcatcgca aagcggattt
     2221 ctctcgccgc gctcgtactt caggatgctg accgcgtcgc ggattccggc caagccaacg
     2281 ccttggacaa ccgagccttc cgcctcataa acaacctcat agatccagcc gggcttgagg
     2341 ccgccggcga cctg
//
LOCUS       DASUGB010000143         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_989800, whole genome shotgun sequence.
ACCESSION   DASUGB010000143 DASUGB010000000
VERSION     DASUGB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989800"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1072)
                     /locus_tag="VFV87_02820"
     CDS             complement(80..1072)
                     /locus_tag="VFV87_02820"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5102713.1"
                     /translation="MSTALAEFNTVPAVWNRRHLLDLENLSAEEITCVLDAAEAFKRA
                     TGGCRGKISVLPGRTLANLFFENSTRTRNSFSLAARRLGADTVEFTASGSSVSKGETF
                     IDTAKTIEAMNVDAVVVRHRTPGTPYLLAKNLNCSVINAGDGPHEHPTQGLLDILTIR
                     EHRGSLAGLTVALVGDIAHSRTARSNIWGLKKLGAHVIVCGPATLVSRHWEEFGVEVA
                     YNLDAILPRCDVLNLLRVQFERQHTRPFPSVREYALLYAMNRQRLAKAKSDILIMAPG
                     PINRGVEITPDVADGPHSVILQQVTNGLAVRMAVLWLVMGVGNRGQGTGDRGRE"
     gene            1183..2034
                     /locus_tag="VFV87_02825"
     CDS             1183..2034
                     /locus_tag="VFV87_02825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5102714.1"
                     /translation="MTRPARICLSSGQEQRLRAWKEALRTARCQIVGDDGDVADEIDV
                     LVIDRPLSDAHLSFDEQRLSSGQTGMIAVGIGLPADVSLPADHSPRELRLACLLLSEI
                     VRLRRQREASRRQEKVLRHLALSDPLTGLPNRRAWDQQLAERLSGAGQGEWCVALLDV
                     DQLHDLNDQRGLLEGDACLRRIADRLTGLVRRGDFVARLGGDEFGVLLEGLNAERAGA
                     QVDLIRAQAGEDGNDAKGFPPLQLTAGWAALTAPASRTAVQEALGHADEALRKAKRAG
                     RNRTQSA"
     gene            complement(2031..2639)
                     /locus_tag="VFV87_02830"
     CDS             complement(2031..2639)
                     /locus_tag="VFV87_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX5102715.1"
                     /translation="MYVPAHFAENDPAALHDAMERYSFATLISRAGGEPFASHLPLLL
                     ERDSGPQGTLIGHMARANPQWREAAGQVVLAVFSGPHAYISPTWYAAEQVVPTWNYVA
                     VHAYGRLELIDDPAAAEAVLRRTIDVYEASQPQPWQLDESPQFALRLVRQIVAFRIPI
                     ERTQGKWKLNQNHPEERRERVVAALESQADENSREIARLMRG"
BASE COUNT          519 a          989 c          942 g          510 t
ORIGIN      
        1 cgatcaacac cgacgtagtg cgatcgagcc cctgtgcagg acagaccata cggccgccgg
       61 tgatgagaat tcgtggcatc tattcgcgtc ccctgtcccc tgtcccctgt cccctgttcc
      121 ccactcccat caccaaccac agcacggcca tcctcaccgc caatccattc gtcacctgct
      181 gcaggatgac gctgtgcggc ccgtcggcga cgtcgggcgt gatctcgacg ccgcggttga
      241 tcggcccggg ggccatgatc aggatgtcgc tcttcgcttt ggccagccgc tggcgattca
      301 tggcatagag cagggcatat tcgcgaacgg acgggaaggg ccgcgtgtgc tgccgctcga
      361 actgcacgcg gagcagattg agcacgtcgc accgcggcag gatcgcatcg aggttatagg
      421 caacctccac tccaaactct tcccagtggc gcgaaaccag cgttgccggg ccgcacacaa
      481 tcacatgagc gccgagcttt ttcaggcccc agatgttcga ccgggcggtc cggctgtggg
      541 cgatgtcgcc aaccagcgcg accgtcagcc cggcgagact gccgcgatgc tcgcggatgg
      601 tcaggatatc gagcaggccc tgcgtcgggt gttcgtgcgg gccgtcgccg gcattgatca
      661 ccgagcagtt caggttcttc gccagcaggt atggcgtgcc gggcgtccgg tggcgaacga
      721 caaccgcatc gacgttcatc gcctcaatcg tcttcgccgt gtcgatgaac gtctccccct
      781 tgctgacgct gctgccggag gcagtgaatt ccaccgtatc ggcccccagc cgccgggcgg
      841 cgagcgaaaa actgttccgc gtgcgggtgc tattctcgaa gaacaggttg gccagcgtcc
      901 ggccaggcag caccgagatc ttcccgcggc agccgccggt ggcccgcttg aaggcttccg
      961 ccgcgtccag aacacacgtg atttcctccg ccgaaaggtt ctccaaatcc aacaggtgcc
     1021 gccgattcca gacggcgggc acggtgttga attcggccag ggcggtgctc acggctgggc
     1081 ggcaaacatg cgagcggcgt gcaaaattgg ggtgattcta gcaccttgcc cgtccctcgc
     1141 aaggacggca atggcgaccc gtctccatta taataatcgg acatgactcg tcccgcgcgt
     1201 atctgtctgt cgagcggcca agagcagcgc ctccgcgcct ggaaggaggc gctgcggacc
     1261 gcgcggtgcc agatcgtggg cgacgatggc gacgtggctg acgagatcga cgtgctggtc
     1321 atcgaccggc ccctctccga cgcccatctg tcgttcgacg agcagcggct ttcgagcggg
     1381 cagacgggga tgatcgccgt cggtatcggc ttgccggctg atgtttcgct gccggccgat
     1441 cattcgccgc gcgagctgcg gctggcctgc ctcttgctct cggagatcgt ccgcctccgc
     1501 cgccagcgcg aagccagccg gcggcaggaa aaagtgctga ggcatctggc gctctccgat
     1561 ccgctgacgg ggctgcccaa ccgccgggcc tgggaccagc agcttgccga gcggctgagc
     1621 ggggcagggc agggggagtg gtgcgtggct ctcttggacg tcgatcaact acacgacctg
     1681 aacgaccagc gcggcctctt ggagggagac gcctgcctcc ggcgaattgc cgatcggctg
     1741 acagggctgg tgcggcgcgg cgacttcgtc gcgcggctgg gcggcgacga gttcggcgtg
     1801 ctgttggagg ggctgaatgc agagcgggcc ggagcgcagg tcgatctaat ccgcgcacag
     1861 gccggtgaag acggcaacga tgcgaaagga ttcccaccac tgcaactgac ggcgggttgg
     1921 gcagctctca ccgcgcccgc cagcaggacc gccgtccaag aggcgctggg ccatgccgac
     1981 gaagccctgc gaaaagccaa acgcgccggt cgcaatcgga cgcaatcggc ttagccgcgc
     2041 atcagccggg caatctcgcg cgagttctca tccgcctgcg attccaaggc cgcaacgact
     2101 cgttcccgcc gctcctcggg atggttctga ttcagtttcc acttgccctg ggtccgctcg
     2161 atcggaattc gaaacgccac gatctgccgc acgagccgca gggcaaattg cggcgactcg
     2221 tcaagctgcc aaggctgcgg ctgcgacgcc tcatacacgt caatcgtccg ccttagcacc
     2281 gcctccgcgg cggctggatc gtcgatcagc tccagtcgcc cgtaggcatg gaccgccacg
     2341 tagttccagg tcggcacgac ctgctccgct gcgtaccagg tcggcgagat atacgcgtgc
     2401 ggaccggaga agaccgccag tacgacctgc ccggccgcct cgcgccactg cggattggcc
     2461 cgcgccatgt gtccgatcag cgtgccctgc gggccgctgt cgcgctccag cagaagcggc
     2521 agatggctgg cgaaaggctc gcccccagcc cgcgagatga gcgtcgcaaa gctgtagcgc
     2581 tccatcgcat cgtgcagggc agcaggatcg ttctcggcga aatgggcagg aacatacata
     2641 agcggctctc agcatggctt gacggcagac ggggcgtgaa cgacgcttga accgggcggg
     2701 gtcgggagtc aattgccgtc gcaatcttca gtcttggaac acgataaccg caattgactc
     2761 ccgaccccat cacacctcca ctcgcgcacg cccaaaatct ctccaggagc ccaccatgtt
     2821 ccgttccccc agccagacaa cctttatgct caccgggttc gccctcctgc ttgtcatggc
     2881 tgccgctatg agttcccgtg ccgccgccga agataaggag cctggcgggg aattgcggca
     2941 cgtcgtgctg gtcaaattca
//
LOCUS       DASUGB010000144         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_851039, whole genome shotgun sequence.
ACCESSION   DASUGB010000144 DASUGB010000000
VERSION     DASUGB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851039"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1121)
                     /gene="pilM"
                     /locus_tag="VFV87_02835"
     CDS             complement(66..1121)
                     /gene="pilM"
                     /locus_tag="VFV87_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX5102716.1"
                     /translation="MFKWLPTRRYSPIGIDVGARSVKLVQLTGDRSRLVDAARVELPT
                     AEESTTPEQQAERISAAVAKGLAGRSFHGREAVVCLSDKLFLQNVRVPKQQGEAFDRQ
                     VAQEASRRLPYSVEDAEIRFIEASDVRQGDSVLREVIVFACQRSELQQALNVCERARL
                     RPVAVDVEPAALARSYAGQYRREEDRQGRALVVQIGYSRTAAMIAQGDELLFVKYIDI
                     GGQHLDAAVARHLSMNLPEAVSLRRHNGDRRADMQDPEIARSVALATRPVLEKLASEL
                     AMCVRYHSVTFRGQPLVRLVLGGGEATPPLLEALGKQLDLPCELSDPFRTFPTVPNLG
                     RKGQWDVAAGLAMRSLN"
     gene            complement(1192..1899)
                     /locus_tag="VFV87_02840"
     CDS             complement(1192..1899)
                     /locus_tag="VFV87_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5102717.1"
                     /translation="MRNRAANTVNAAAFARTRAGAAHPHSGECGYESASPQSAIRNPQ
                     SAIATRGFTLIEVLVTLLLLAILAAALIPQLSSDLPERLQAGAEIVAADLEYARSLAV
                     ANNSTYRITFDTGENLYYLQHTGSNNLLNALPPSPFRQNDDPPDRQTTDLDLLPIPPP
                     TIRLIAVAQGAAGPLANPQIDYQPLGGTQGQEETVIWLGCGGGTSARFVSIHIHPVTG
                     LAEIGPLLTALPADIAP"
     gene            complement(1880..2131)
                     /locus_tag="VFV87_02845"
     CDS             complement(1880..2131)
                     /locus_tag="VFV87_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102718.1"
                     /translation="MTSSSSLRWSLLWAANFAVLGMLGFYSIGGAAPQAGQPPFANSI
                     EQRAEMVQELREIKELLKEQNALLRGGADKAEAREKPRR"
BASE COUNT          364 a          705 c          766 g          385 t
ORIGIN      
        1 cgcaggtcct cgcgctggcg atagatgtcg ggcaggaagt cgatgttctt catggttctg
       61 gggacctaat tcaaactccg catcgccagg ccggccgcca cgtcccattg ccccttgcgt
      121 cccaagttcg ggacggtcgg gaacgtgcgg aacggatcgc tcagctcgca cggcaaatcg
      181 agctgcttgc ccagcgcttc caagagcggc ggcgtcgctt cgcccccgcc cagcaccagc
      241 cgcaccagcg gctggccgcg gaaggtgacg ctgtggtaac ggacgcacat cgccagctcg
      301 ctggcgagct tttcgagcac cggccgcgtg gccagggcga cgctgcgggc aatttcgggg
      361 tcctgcatat cggcccgccg gtcgccgttg tggcggcgga gcgagacggc ttccggcagg
      421 ttcatgctga ggtggcgggc gacggccgca tcgagatgct ggccgccgat atcgatgtat
      481 ttgacgaaca gcagctcgtc tccctgggcg atcatcgccg ccgtgcggga atagccgatc
      541 tgcacgacca gggcccggcc ctggcggtct tcttcgcggc ggtactggcc ggcgtagctg
      601 cgcgcaaggg cggccggctc gacatcgacc gccaccggcc gcaggcgggc ccgctcgcag
      661 acgttcagcg cctgctgcaa ttcgctccgc tggcaggcga agacgatcac ctcgcggagg
      721 acagaatccc cctgccgcac gtcggaagcc tcgatgaacc ggatctcggc gtcctccacg
      781 ctgtaaggca accgccgcga agcctcctgc gcgacctgcc ggtcgaacgc ttcaccttgc
      841 tgcttgggga cgcgaacgtt ctgcaaaaag agcttgtcgc tcaggcagac gaccgcttcg
      901 cggccatgga acgagcggcc cgcaagcccc ttggccaccg cggcggaaat ccgctccgct
      961 tgctgctcgg gggtggtcga ttcttcagcc gtcggcagct cgacccgggc cgcgtcgacc
     1021 agccgcgagc ggtcgcccgt aagctggacc agcttcacgg agcgcgcccc gacatcgatg
     1081 ccgatgggcg aatagcggcg ggtgggaagc cacttgaaca tggggcgttt gggctctcgt
     1141 gtcgtaggac aggttttaac ctgtcccttt cggacgggtt ggaaacccgc cctacggggc
     1201 gatgtcggcg ggcagggccg tcaacagcgg gccgatttcg gccagcccgg tgacagggtg
     1261 gatgtggatc gaaacgaacc gcgcgctggt tccgccgccg cagccgagcc agatgaccgt
     1321 ctcttcctgg ccttgcgtcc cgcccagcgg ctgataatcg atttgcggat tggccagcgg
     1381 gccggcggcg ccctgggcga cggcgatcag gcggatggtc ggcggcggaa tcggcaagag
     1441 gtccaggtcg gtcgtctgcc ggtcgggcgg atcgtcgttc tgacggaaag gcgaaggcgg
     1501 cagcgcattc aaaaggttgt tgctgccggt gtgctgcagg taatagagat tctcgccggt
     1561 gtcgaaggtg atgcggtagg tggaattatt cgcgaccgcc agcgagcggg cgtattccag
     1621 gtcggccgcg acgatttccg ccccggcctg caggcgctcg ggcagatcgc tggaaagctg
     1681 cgggatgagg gccgccgcga gaatcgcgag cagcaggagc gtcaccagga cttcgatcag
     1741 cgtgaagccg cgggtggcaa tagcggattg cggattgcgg attgcggatt gtgggctggc
     1801 gctttcgtag ccgcactcgc cagagtgcgg gtgcgccgcc cctgcccgcg ttctggcgaa
     1861 cgcggctgcg ttgacggtgt tagcggcgcg gtttctcacg ggcttcggct ttgtcggcgc
     1921 cgccgcggag cagggcgttc tgctctttca gcagctcctt gatttcgcgc agctcctgga
     1981 ccatttcggc ccgctgctcg atggaattgg cgaacggggg ctggcccgcc tgaggggcag
     2041 cgccaccgat gctgtaaaaa cctagcatgc ccaggacggc aaaattggcc gcccacaaga
     2101 gcgaccatcg cagcgaagaa gaactcgtca tctttgattc tcctgataca accggcaatt
     2161 tcagaacagg cgacaagtgt cactggaccg ctggtaccca cgctctgcgt gggaacccac
//
LOCUS       DASUGB010000145         9736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_17198,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000145 DASUGB010000000
VERSION     DASUGB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17198"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFV87_02850"
     CDS             complement(<1..734)
                     /locus_tag="VFV87_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEX5102719.1"
                     /translation="MAEPMTTVGTTAVALSAPALKKAIELVVSDIYAQAKGSIAEKVK
                     RWRNRGRIDTLYKKLKQIRYVKTIWQLDKEIDLLRFYHPTSLVVEGSRIQINDLSKVP
                     YGGNFIIEGTVGQGKSIFLRYLASCELAKGQAVPLFVELRRVASQHSLNGLLLDELRN
                     LGLDSDESVLEFLASTGRLILFLDGFDEIHQDRRMEVISSLEWHRPISIATLSGADAV
                     ENFAGGLVVEGFTGTVVDTFYGLRQIV"
     gene            complement(772..2310)
                     /locus_tag="VFV87_02855"
     CDS             complement(772..2310)
                     /locus_tag="VFV87_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5102720.1"
                     /translation="MLAKLHTFSLVGIEAVPVEVEVDVSAGALPKTILVGLPEAAVKE
                     SVHRVERALANSGFVRPRDRVVVALAPADLPKNAASFDLPITLGTLAGSGQLVSELFE
                     QYAVVGELSLEGTTRPVKGALSMAIAAAQQKGLKGVVISAESAAEAAVVEEIEVIPVS
                     TLAQAVGFFTGTLQIDPTPSRIGELFQELSKYDEDFADVRGQEMAKRAITVAAAGKHN
                     ILMLGPPGSGKTMLAKRVPTICPPLTAPESIETTRIYSSVGRLQPGQPLMARRPYRSP
                     HHTISEPGLVGGGNPPVPGEISLSHNGVLFLDELPEFNRRTLEVLRQPLEDGTVTISR
                     AMNATTFPADFQLVAALNPCPCGFRNDPRRECHCSIPQIERYMAKISGPLLDRIDIHS
                     EVPAVPFKELSGERAGTTSAMMREQVVAARALQAERFAGSRTRYNGQMTSRQLRKFCK
                     LDDVCQEQMSASVNDMGLSARAHDKVLRVARTIADLDNAPDIQQHHLQEAINYRMLDR
                     QMWT"
     gene            2562..3290
                     /locus_tag="VFV87_02860"
     CDS             2562..3290
                     /locus_tag="VFV87_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102721.1"
                     /translation="MSRFWLSHAIGRSNAPWVQPFIREVWQFRTARRREVVLHYAPEL
                     EREFIPETLLLLSKEELTSLARKFGFRLPRVNVFLFSSIGCVTEIFGPEYAGLALPAF
                     NAIVVGEHDTVSEHVRHELVHLFASRWNLFAPPLLTEGLAVHLQQVWCGYPIATLFRR
                     FGSRSEWTLRSLLDRNFFFDRTYRHACYMIAGSFTSFLIDEFGWDAYRRLYRWSRPHL
                     VEWAFRRSLGVSFDEAESRWRGRL"
     gene            3357..4517
                     /locus_tag="VFV87_02865"
     CDS             3357..4517
                     /locus_tag="VFV87_02865"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5102722.1"
                     /translation="MSKTSRRTLLTATSAAAALAILPSRKGFAAAPQGEILETKVISL
                     DRRFYSGWPTVCRRASGQLIVTCSGGREAHVCPFGRVEMITSDDDGQTWTWPRTLLDG
                     PIDDRDSGCLETAKGTLLVTTFTSLAYEPGLAAANKAGNWEAEKLARWNAVNNRLSPE
                     ARKQQLGVWMIRSTDGGLNWSPKYDCLVSSPHGPIQLADGRLLYAGKDLWRAGTRVGV
                     AESTDDGQTWKWLADIAPREGDKHSEYHELHAVETNDGRLVVQIRNHNKSNERETLQC
                     ESKDGGRSWTEPKSIGVWGLPSHLLKLRDGRILMSYGHRRAPFGNQVRLSTDHCRTWS
                     EPIILSGDGIGGDLGYPSTVELADGTLLTVWYETMQGSPLAVLRQAKWKLAL"
     gene            4672..4998
                     /locus_tag="VFV87_02870"
     CDS             4672..4998
                     /locus_tag="VFV87_02870"
                     /inference="COORDINATES: protein motif:HMM:NF017958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HEX5102723.1"
                     /translation="MNGRLEDPVLRSSRREGLLVLGIWAVACVYTVGTCYVFGYNRDP
                     DSIKYVAGFPDWVFYGIIVPWTVCTLLCYWLANFFIRDEDLGEEQAESDLGSLEDALG
                     GEGEHV"
     gene            4991..6676
                     /locus_tag="VFV87_02875"
     CDS             4991..6676
                     /locus_tag="VFV87_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX5102724.1"
                     /translation="MFELLAQAADASSAAPAVGIGAILSLILFIVASVWLGTVAQRVV
                     ERSRFIQGYFQGNRGLGAWTIALTATVQSGGTFMGFPSLVYSSGWVVALWIASYMAVP
                     LTGFAVIGKRIAQISRRTGAVTMPDLFRARYASPTAGLITSLLIILFLSIMMVAQFKA
                     GAVVMKIAWPGTTSLALAEDAESSIDTVYLIGLAVFGLTVVGYTLIGGFLAAVWTDLL
                     QSVMMFFGVMILVFLAMWAVGGLENASRKAVSNLEQVALERGKTPEEAQFVASQYISG
                     PGLKGDGSAFLPLTMAISFFFFWPFAGMASPASVVRIMACKDTSVLRKSIFLLAVYNM
                     AIYIPLIAVCIAARAMMPNLEKTDEVVPRMALALTADIPLGSWISGLILAAPFGAIMA
                     TVSCYLLVIASGLVRDVYQRFIHPKANEHELRRLTYFTMAIVGGIAAVLNISPVEKLQ
                     MLVVFSSGCAASSFLMPTLMMCYWRRATAAGTIAAMLSGAGASLALNLAGLFLAYKNT
                     GQFAKFTPYALAGFEPLLWGLLVSLVVGVIVSLFTRPPEEKLVSKLFDAPEPA"
     gene            6759..8594
                     /locus_tag="VFV87_02880"
     CDS             6759..8594
                     /locus_tag="VFV87_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102725.1"
                     /translation="MHRRILLFVALVSLWFTPSHAQSQDRFEFTRLLAHWAEYNHESY
                     LPFVLEAEPDVVQLGFYGGHFWSLAHTDVYGGYPAHFPVQGLDECGAWFEEKNKALHA
                     KNIKVVGHFNVEFLVGDIDGPKGPTGFFKFYRDLWDEKQLGPKPVADPVTMLERGPNG
                     KPIGQEGYGIGGMHEYWACLRNPDWQKVLQAWVKRGIDRGVDGYIANYFYKHDCHCEH
                     CQAEFRAYLKERHSAQELISKFDIKNVDSHTFGEMAYWHDPKESKPLKREMLRWAQIS
                     NKRVFDQVFHEYGRSLKPDLVSAQWNHLGNFSQIAGDERCLLPANLWGKDETYLWYST
                     GGAACATDIPAGNLGEGTLQARYIRGMFDDKPYTLGKYEHTRIRVTIAELAANGGAPM
                     GFYTRFTEPEARTEIVRYYQFLKRYEEIFRGNRSHAEAVLVYPRIAVAAGNVEPVEQF
                     RQAGKRLLNAHVLFDVCSDEVEQPAGDAATVIRTTEEAEQAAGTVSSRFDAPATVRIS
                     ASRPAKGDELDIHFVNYNRTEPPKNKDGTPSTGRGIMDEQPIESPEIKCDVLLPKGFK
                     AAIIEAITPESPDPQPISFTQSDRRIQFTQPKFLVYSIARIKLAR"
     gene            8707..>9736
                     /locus_tag="VFV87_02885"
     CDS             8707..>9736
                     /locus_tag="VFV87_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102726.1"
                     /translation="MWSPSPAPAQESLWIEAEHLDGFHGYCWPMGKPEMKKTDGHWAL
                     SGPGWAAEWNQGGESGFLSIATAADDDKAVATKAIEIPVAGKYFVWVRYGDWRETTER
                     FQIQLEQPGRPLFTASFGEKPVIEEDNEMKLYWNWAFGWGSAEAELAKGPATLKLLST
                     TKETQPRQIDVIVLTTDAAYRPRIKDRPKHYAWDVLDSYRGGKIPADLEPLARRRPDF
                     QQPATAEPPPSWKLKTFRDQGLIYLWNVSHTNPAETWLGDKPDRVMVPYNVSDPDVRA
                     AFEKKYAGRADVPIFGDPRVTPTFHGVGPGIFAEDPKTGEVNDAGQRFARWLDANPQR
                     NWAMMMNYH"
BASE COUNT         1795 a         3042 c         3027 g         1872 t
ORIGIN      
        1 acgatctggc gcagaccgta aaacgtatca acgaccgtcc ccgtaaatcc ctcaactacc
       61 aaacccccag cgaagttttc cacggcatca gccccgctca aggttgcaat tgagattgga
      121 cggtgccatt ccaacgacga gattacttcc attcgcctat cttgatgtat ttcatcaaat
      181 ccatccagga ataggattag acgacctgtc gatgccagaa actccagtac cgattcatca
      241 ctgtcgagcc cgaggtttcg caactcgtca agtaacaaac cgttcaagct atgctgtgac
      301 gcgacgcgcc ttagctcgac aaatagtgga acggcttgac ctttcgccaa ttcgcagctc
      361 gcaaggtagc gtaggaagat ggactttccc tggccaactg tcccctcaat gataaagttc
      421 ccgccatagg gtacttttga caagtcattg atctggattc tactgccctc gacgaccagc
      481 gatgtcggat ggtagaagcg gagcagatct atttccttgt cgagctgcca aatcgtcttt
      541 acgtagcgaa tctgctttag cttcttgtat agggtgtcaa tgcggcctct gttgcgccac
      601 cgtttgactt tctcagcaat actgcctttc gcttgtgcgt atatgtcgct gacgacaagc
      661 tcaatagcct tttttagggc tggagcggat agcgctactg ccgtagtacc gacagtcgtc
      721 attggttcgg ccattgaatg ggagggcaag tgaattagga gcttgaaata tttacgtcca
      781 catctgccta tccagcatcc ggtagttgat tgcttcctgc aggtggtgct gctggatgtc
      841 aggcgcgtta tcgaggtcgg cgatcgtgcg ggcgacgcgg aggactttgt cgtgggcccg
      901 ggcagagagc cccatgtcgt tcacgctcgc gctcatctgc tcctggcaca cgtcgtccag
      961 cttgcagaat tttctcagct gccggctggt catctggccg ttgtaacgcg tcctgctgcc
     1021 ggcgaatcgc tcggcctgca gcgcgcgggc tgccaccacc tgctcgcgca tcatcgcgct
     1081 ggtcgtgccg gcccgctcgc cgctcagctc cttgaacggc acggcgggga cttcgctgtg
     1141 gatgtcgatc cggtcgagca gcgggccgct gatcttggcc atgtaccgct cgatctgcgg
     1201 aatcgagcag tggcattccc gccgcggatc gttccggaac ccgcacgggc acgggttgag
     1261 cgccgcgacg agctgaaaat cagccgggaa cgtcgtcgcg ttcatcgccc gcgagatcgt
     1321 gaccgtgccg tcctccagcg gctggcgaag gacttccagc gtgcggcggt tgaactcggg
     1381 cagctcgtcg aggaacagca cgccgttgtg cgagagcgag atttcgcccg gcaccggcgg
     1441 attgccgcct ccgaccaagc ctggttccga tatcgtgtga tgcggcgagc ggtacggccg
     1501 gcgggccatc agcggctgcc ccggctgcag gcggccgacc gagctgtaga tgcgggtcgt
     1561 ctcgatcgat tccggcgcgg tcagcggcgg gcagatcgtc ggcacccgct tggccagcat
     1621 cgtcttgccg ctgccgggcg gcccgagcat caaaatattg tgcttgccgg cggcagcgac
     1681 ggtgatcgcc cgcttggcca tttcctggcc gcggacgtcg gcgaaatcct cgtcgtactt
     1741 cgacagctcc tggaacagct cgccgatccg cgacggcgtc ggatcgatct gcagcgttcc
     1801 cgtgaaaaag ccgaccgcct gagccagcgt cgagaccgga atgacctcga tctcctcgac
     1861 gacagccgct tcggccgcgc tttcggccga gatgacgact cccttcagcc ctttctgctg
     1921 ggcggcggcg atcgccatcg agagggcgcc cttgaccggc cgcgtcgtcc cttcgagcga
     1981 caattcaccg acgaccgcgt actgctcgaa cagctccgac acaagctgcc cgctgccggc
     2041 cagcgtgccg agcgtgatcg gcagatcgaa cgacgcggca ttcttcggca gatcggccgg
     2101 cgcgagcgcg acgaccacgc gatcgcgcgg gcggacgaag ccgctgttgg ccagggccct
     2161 ctcgacgcgg tggacgctct ccttcacggc cgcttccggc aagccgacga ggatcgtctt
     2221 cggcagcgct cccgccgaga catccacctc cacttccacc ggcaccgcct caatgccgac
     2281 cagcgagaac gtgtggagct tggcgagcat ggcagaccgc ggaggaaaac cacgaatggc
     2341 acgaatgaca cgaatgcgtc tcagggggct gacgcccccc gctcgccgtt gcggcaattg
     2401 tcgcgggtcg aaagccagtc cgcaagttgc ggcgcggaat cgcggcgggt tatgatcgct
     2461 gcggagaatc tatgaaacgt ctcgaaacgg tagtttcacg agcaatcagg tcgggccatt
     2521 ccgacacatc gttcggaggc cggtgcgatt cgatacgtcg cgtgagccgc ttttggctat
     2581 cgcacgcaat cggccgatcg aacgcaccgt gggttcaacc gttcattcgc gaagtctggc
     2641 agtttcgcac cgcccgacgg cgcgaggtcg tgctgcatta tgcgccggag ttggagcgcg
     2701 aattcattcc tgagacgttg ctgctcctat cgaaagagga actgacgtcg cttgcaagga
     2761 agttcggctt tcgcctgccg cgcgtgaacg tcttcctgtt ttcatccatc ggatgcgtta
     2821 ccgagatttt cggtccagag tacgccggct tggctctgcc cgcgttcaac gcgattgtcg
     2881 ttggcgagca tgacaccgta agtgaacacg ttcggcatga gcttgtgcat ctctttgcca
     2941 gtcgatggaa tctgttcgcg ccgcccctat tgaccgaggg gctggccgtg catctgcagc
     3001 aggtctggtg cggttatcca attgcaacgc tctttcggcg cttcggcagt cgatccgaat
     3061 ggactttgcg ctccctcctc gatcggaact ttttcttcga ccgcacgtac cgccacgcgt
     3121 gctacatgat cgctggcagc tttacgagtt ttctgatcga cgaattcggc tgggacgcct
     3181 atcgaaggct ttaccgctgg agccgtccgc atcttgtaga gtgggcgttc cgccggtcgc
     3241 taggagtctc atttgacgaa gcggaatcgc ggtggcgcgg tcggctatga tttggcaggc
     3301 cacgccaatg tgcttctgct agttgactac ctcaaaatgt tcgtggaagg agagccatgt
     3361 ccaaaacctc gcgtcgtact ttgctcaccg ccacgtccgc tgcggcggct cttgcgattc
     3421 tgccctcgcg gaagggcttt gccgcggccc cgcaggggga gattctggag acgaaggtca
     3481 tcagcctgga ccggcggttt tacagcgggt ggccgacggt ttgccggcgg gccagtggcc
     3541 agctcattgt gacctgctcc ggcgggcgcg aggcgcatgt ctgcccgttc ggccgcgtcg
     3601 aaatgatcac gagtgacgac gacggccaga cctggacctg gccgcggacg ctgctcgacg
     3661 ggccgatcga cgaccgcgac agcggctgcc tggaaacggc caaggggacg ctgctggtga
     3721 cgacattcac gtcgctcgcc tatgagccgg gcctcgcggc tgccaacaag gcgggcaact
     3781 gggaggcgga aaagctcgca cgctggaatg ccgtcaacaa tcgcctctcg cccgaagccc
     3841 gcaagcagca gctcggcgtc tggatgatcc gctcgaccga cggcgggctg aactggtcgc
     3901 ccaagtacga ctgcctcgtc agcagtccgc acgggccgat ccagcttgcg gacggccggc
     3961 tgctctatgc cggcaaggac ctgtggcgcg cggggacgcg cgtcggcgtc gccgaatcga
     4021 ccgatgacgg ccagacctgg aagtggctgg ccgacatcgc cccgcgggag ggcgacaagc
     4081 actcggaata ccacgagctg cacgccgtcg agacgaacga cggccgcctc gtcgtccaga
     4141 ttcgcaatca caacaagtcg aacgagcgcg aaacgctgca atgcgaatcg aaagacggcg
     4201 gcagaagctg gaccgagccg aagtcgatcg gagtctgggg cctgccgtcg cacctgctga
     4261 agctgcgcga tggccgcatc ctgatgagct acggccaccg ccgcgccccc ttcggcaatc
     4321 aagtccgcct cagcaccgac cactgccgta cgtggagcga accgatcatc ctcagcggcg
     4381 atggcatcgg cggcgacctt ggctatcctt ccaccgtcga gctggccgac ggcaccctcc
     4441 tcaccgtctg gtacgagacg atgcaaggct cgccgctggc cgtgttgcgg caagcaaaat
     4501 ggaaattggc gctgtaggga acgccctccg tggcgttccg ggaaccgccg ccgacgtcgc
     4561 tcgccccgca tggcacgcca gggacggcgt gccctacagc gcatttgctt ctcgcttccg
     4621 acgcaggtta tattgcgacc tcccgcccgt cccctcctcc cacctcccgc catgaacggt
     4681 cgcttggaag atcctgtcct gcgcagctcg cggcgcgagg ggctgctggt gctgggaatc
     4741 tgggccgtgg cctgcgtcta taccgtcgga acgtgctacg tcttcggcta caaccgcgat
     4801 cccgactcga tcaagtacgt cgccggtttt ccagattggg tcttctacgg catcatcgtg
     4861 ccgtggacgg tttgcacgct cctctgctat tggctggcga acttttttat tcgcgacgaa
     4921 gacctgggcg aggagcaggc tgaatcggac ttgggctcgc tcgaagacgc cctcggcggg
     4981 gagggcgagc atgtttgagc tcctcgcgca agccgctgac gcgtcgtccg ccgcccccgc
     5041 tgtcgggatc ggggcgatcc tgtcgctgat cttgttcatc gtggcctcgg tctggctcgg
     5101 cacggtcgcg cagcgggtcg tcgaacgcag ccggttcatt cagggctatt ttcaaggcaa
     5161 ccggggcctc ggcgcctgga cgatcgccct caccgccacc gtccaaagcg gcgggacgtt
     5221 catggggttt ccgtcgctgg tctattcctc cggctgggtc gtcgccctct ggatcgccag
     5281 ttacatggcc gtgcccctga ccggatttgc ggtgatcggc aagcggatcg cccagatttc
     5341 gcggcggacc ggcgcggtga caatgcccga cctgttccgc gcccgctacg ccagcccgac
     5401 agccggcctg attacatcgc tgctgatcat tctgttcctt tcgatcatga tggttgccca
     5461 gttcaaggcg ggggcggtcg tgatgaaaat cgcctggccc ggcacgacca gcctcgcgct
     5521 cgccgaagat gccgaaagca gcatcgatac ggtctatctg atcggactcg ccgtgttcgg
     5581 gctgacggtc gtcggctaca cgctgatcgg cggctttctg gcggccgttt ggacagattt
     5641 gctgcagagc gtgatgatgt tcttcggcgt gatgatcttg gtttttctgg cgatgtgggc
     5701 ggtgggcggg ctcgagaatg cgtcgcgaaa ggcggtgagc aatctcgagc aagtggcgct
     5761 ggagagaggg aagacgccgg aggaagccca attcgtcgcc tcgcagtaca tctccggccc
     5821 gggcctcaaa ggcgacggct ccgcgttcct gccgctgacg atggccattt cgttcttttt
     5881 cttctggccg ttcgcaggca tggcgtcgcc ggcgagcgtg gtgcggatca tggcctgcaa
     5941 ggacaccagc gtgctgcgga agtcgatctt cctgctcgcc gtctataaca tggcgatcta
     6001 cattccgctc atcgccgtct gcatcgcggc gcgggcgatg atgcccaacc tggagaagac
     6061 cgacgaggtg gtgccgcgga tggcgctggc cctcacggcg gatattccgc tggggtcgtg
     6121 gatctccggg ctgattctcg ccgcgccgtt tggcgcgatc atggccacgg taagctgcta
     6181 cctgctcgtg atcgcttcgg gcctggtgcg tgacgtctat cagcggttca tccaccccaa
     6241 ggcgaatgag cacgagctgc ggcggctgac ctatttcacg atggcgatcg tcggcggcat
     6301 cgccgcggtc ctcaacatca gccccgtcga gaagctgcaa atgctcgtgg tctttagctc
     6361 cggctgcgcg gcctcgtcgt tcctgatgcc gacgctgatg atgtgctatt ggcggcgagc
     6421 gacggcggcg ggcacgatcg ccgcgatgct gtccggtgcg ggggcttcgc tggcgctaaa
     6481 cctcgcaggc ctgtttctgg cctacaagaa tacgggccaa ttcgccaagt tcacgcccta
     6541 cgccctcgcc ggctttgagc cgcttctgtg gggcctgctc gtgtcgcttg tcgtcggcgt
     6601 gatcgtcagc ctgttcacgc ggccgccgga ggagaagctg gtcagcaagc tgttcgacgc
     6661 gccggagccg gcgtgaaaat tggcacgcgg gaggataacc acgaagccac gaaggtcaca
     6721 aaggagtagg tgttcgtaaa ccgcgagggc cgattttcat gcacagacga atcctcctct
     6781 tcgtggccct cgtgtctttg tggttcactc ccagccacgc tcaatctcag gaccgcttcg
     6841 aatttacgcg gctcctcgcc cattgggccg aatataacca cgagagctac ctgccattcg
     6901 ttctggaagc cgagccggac gtggttcagc ttggctttta tggcgggcac ttctggagcc
     6961 tggcgcacac cgacgtttat ggcggctacc cggctcattt tcccgtgcag ggattggacg
     7021 aatgtggggc ctggtttgaa gagaagaaca aagcgctgca cgccaagaac atcaaagtcg
     7081 ttggccactt caatgtggag ttcctcgtcg gcgacatcga cggtcccaaa ggcccgaccg
     7141 gctttttcaa gttctaccgt gacctgtggg acgagaagca gctcgggccc aagcccgtgg
     7201 ccgatccggt gacgatgttg gagcgcggtc cgaacggcaa gccgatcggg caggagggtt
     7261 acggcattgg cgggatgcac gaatactggg cctgcctgcg gaatcctgac tggcagaagg
     7321 tgctgcaggc gtgggtcaag cgcgggatcg accgcggcgt cgatggctat atcgccaact
     7381 acttctacaa gcacgattgc cactgcgagc attgccaggc cgagtttcgc gcttatctga
     7441 aggagcggca ctcggcgcag gagctgatat cgaagttcga tattaagaat gtcgattcgc
     7501 acacgttcgg cgagatggcc tattggcacg atccgaagga atcgaagccg ctcaagcgcg
     7561 agatgctccg ctgggcgcag atttccaata agcgcgtctt cgaccaggtc ttccacgagt
     7621 acggccgctc gctgaagccg gacctggttt cggcccagtg gaaccacctc ggcaacttca
     7681 gccagatcgc gggcgacgag cgctgcctgc tgccggcgaa cttgtggggc aaggacgaaa
     7741 cctatctgtg gtacagcacg gggggcgcgg cctgcgcgac cgacattccc gccggcaacc
     7801 tgggcgaagg gacgctgcag gcccgctaca tccgcggcat gttcgacgac aagccctaca
     7861 cgctcggcaa atacgagcac acccgcatcc gcgtcacgat cgccgagctg gccgccaacg
     7921 gcggcgcgcc gatgggtttt tacacgcggt tcaccgagcc ggaggcgcga acggaaatcg
     7981 tgcggtatta ccagttcctg aagcggtatg aggaaatctt ccgcggaaat cgttcgcatg
     8041 cggaagccgt cctcgtgtat ccgcgcatcg cggtcgccgc agggaacgtt gagcccgtcg
     8101 agcaattccg gcaagcgggc aagcgattgc tcaatgcaca cgttctgttt gatgtctgtt
     8161 ccgacgaggt cgagcagccg gccggcgatg cagcaacggt catccgcacg accgaggagg
     8221 ccgaacaagc agcggggacg gtgtccagcc ggttcgacgc acccgcaacc gtccgcatct
     8281 ccgccagcag gcccgcaaaa ggcgacgagt tggacatcca cttcgtcaac tacaaccgca
     8341 ccgagcctcc caagaacaaa gatggcaccc ccagcactgg ccgcggcatc atggacgagc
     8401 agccgatcga atcgcccgaa atcaaatgcg atgtgctgct gcccaaggga ttcaaagcag
     8461 cgatcattga agcgatcaca ccagaatcgc cggatccgca gccaatctcc ttcacgcaat
     8521 ccgatcggcg aatccaattc acgcagccca agttcctggt ttattccatc gcccgaatca
     8581 aactggcgag gtagcgtcgg caggaatgac cacaaagaca ccaagaacac gaagaagagt
     8641 agcagagaca tgggctatcg acgactcaac aacttagtaa ttcccctctt cgtgtccttc
     8701 gtgtctttgt ggtcaccttc tcccgcccct gcccaggagt cgctctggat cgaagccgag
     8761 catctcgacg gctttcacgg ctattgctgg ccgatgggca agccggagat gaagaagacc
     8821 gacgggcact gggcgctttc cggccccggc tgggcggcgg aatggaacca ggggggtgag
     8881 agcggctttt tgtcgatcgc caccgcggct gacgacgaca aggcggtcgc gacgaaggcg
     8941 atcgagattc ctgttgccgg caagtacttc gtctgggtcc gctacggcga ctggcgcgaa
     9001 acgactgagc ggttccagat ccagctcgag cagcccggcc ggccgctctt taccgccagc
     9061 ttcggcgaga agccggtcat cgaagaagac aacgagatga agctgtactg gaactgggcc
     9121 ttcggctggg gctcggctga ggcggagctc gcgaagggac cggcgacact gaagctcctt
     9181 tcgaccacaa aggagaccca gccgcggcag atcgatgtga ttgtactgac gacggacgcc
     9241 gcctatcgtc cgaggattaa ggaccggccg aagcactacg cctgggacgt gctcgatagc
     9301 tatcgcggcg gcaagattcc agcggatctg gagccgctcg cccgccgccg gcccgatttc
     9361 cagcaaccgg cgaccgccga gccgccacca agctggaagc tgaagacatt ccgcgaccag
     9421 ggcctgattt acctttggaa cgtcagccat acgaaccccg cggaaacgtg gctgggggac
     9481 aagcccgacc gggtcatggt cccgtacaac gtcagcgatc cggacgtgcg ggctgcgttc
     9541 gagaagaaat acgcgggccg cgccgacgtg cctatcttcg gcgacccgcg cgtgacaccc
     9601 acgttccacg gcgtcggccc cgggatcttc gcggaagacc caaaaaccgg cgaagtcaac
     9661 gacgccgggc agcgcttcgc ccgctggctc gatgccaatc cgcagcggaa ctgggccatg
     9721 atgatgaact atcaca
//
LOCUS       DASUGB010000146         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1962779, whole genome shotgun sequence.
ACCESSION   DASUGB010000146 DASUGB010000000
VERSION     DASUGB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962779"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFV87_02890"
     CDS             complement(<1..234)
                     /locus_tag="VFV87_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102727.1"
                     /translation="MDETPLDAAIEEPSAANESPPRAQTWRDAVDNPWLMLAMLFFVT
                     AALGLPFLWISRGFSMFWKIVLTVVVTAWTVLIL"
     gene            315..719
                     /locus_tag="VFV87_02895"
     CDS             315..719
                     /locus_tag="VFV87_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102728.1"
                     /translation="MNSARSDDPWPAGSYEKWVEEPLSDVLVRANPFQREFLQRLLAE
                     VPALAQQLVLRRVRRRFHPDVELYSEPDNGISADFEHERSRWGFSIDAETNVICVWNM
                     NGQGEYADWNGTGRDSQGDAIRQIREFIARDP"
     gene            734..1237
                     /locus_tag="VFV87_02900"
     CDS             734..1237
                     /locus_tag="VFV87_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102729.1"
                     /translation="MPSSARQPCESGLRIRIAPEEVSFTDWPLRQRPGGSLLALALAG
                     GVSWLVGWAAGRWEVGAIAAAALAFTLWQIWLPVRYEIDGRGIAQLYFGRWRRRIPWS
                     AIRQYEVRSDGVLLVPDAAVTPLSPLRGLYLHWGAHREEVLANLEYYVHSWRSPAGNS
                     IYRRPSP"
     gene            complement(1273..2316)
                     /locus_tag="VFV87_02905"
     CDS             complement(1273..2316)
                     /locus_tag="VFV87_02905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102730.1"
                     /translation="MSPAIRQFVRHSAEQLLQNLKAIEGDLAGGDLQQHYAAVKAVLD
                     ECLAGLDSLGLRGPDNRLPSSELWNVAGDVLQRGWLQNQARTKPRGYAGDFEMLARIY
                     ENRLCEDPLGRLFDRYFQEEAAPRAVRHRMAMMTEWIVDLVRNSPSPPTSLPQGARGA
                     RIAVVGSAFGLEVRDALMRLDESERQRLSVTLLDLDPSAIEYARDSLQGLIGEDRLTA
                     VSANLFRLPDRPALAAPLNESDLLLCPGLFDYLDKAAAAAMLRTFWERLAPGGRVAVF
                     QFAPHNPTRTYMEWLGNWYLTYRTSDELAHLAAAAGLPAECVEFSSEPLGVDLLLVAT
                     QKGSGVNCDNGLP"
     gene            2400..3755
                     /locus_tag="VFV87_02910"
     CDS             2400..3755
                     /locus_tag="VFV87_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102731.1"
                     /translation="MPVVVACRCGQRFAAQDHLFGTQVACPACGSPLVIPHPQMQPAP
                     MPAPAMPAPASAPLRGAYPAPQPQNAPAPASGGASSDWKLIGLILGGAGLLAVVVVIG
                     LAIAFSGPPRNPAALANVPAGAGPPPSSAPPPPPSGSALPPTPTAAPPGSSAAGAATT
                     PANSNPFAAAAASTSSTSSPESSNSRPAASQSSSASTQPPRSAFRDDPTAAGQGIAFL
                     PTAVDSWHGDQTARRGLLRVGDSEPAFQHFSWMTQLLPFLGHQKEYDRIDFRKELFTE
                     TNLEVGLEVIPAFQNPLDDRKTWKGHPFQGLALTHFAGMSGIEDTRNVVAATLPRSDP
                     RAGVFGYDEVARPEQITDGTSQTIMIVGTGALANPWIMGGGGTIRGAREPYFDPISGL
                     GTRNLPQAGTLAVMADGSVRFISASVDPQAFRAMCTIHGRDTVDLERAAQPFLLDSIQ
                     K"
     gene            3776..>4118
                     /locus_tag="VFV87_02915"
     CDS             3776..>4118
                     /locus_tag="VFV87_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102732.1"
                     /translation="MRHQIALALVIFGFAASPAAADVVFYRLPTSSGSVVVLEGSATV
                     NPGGTVTFSHNRFGKIHFDLENTEIRKAPTVREQFSRQLNRAGNDANKLLEAAHWALR
                     HGLLPQFYSAVD"
BASE COUNT          726 a         1380 c         1269 g          743 t
ORIGIN      
        1 caagatcagc acggtccagg ccgtcacgac tacggtcagg acgatcttcc agaacatcga
       61 aaacccgcgg ctgatccaga ggaagggcag gccgagcgcg gcggtgacga agaacagcat
      121 cgccagcatc agccagggat tatcgaccgc atcgcgccag gtttgcgcac gcggcggcga
      181 ttcattcgcg gcggacggct cttcgatggc agcgtcgagg ggtgtttcgt ccatttcgga
      241 tcgtcagtat atcgcctcga aatgctgggg gaaacgattg caggtcgtga ggctgcgcga
      301 caaaaattgg ttacatgaac tccgcccgca gcgacgaccc gtggcccgca ggatcttacg
      361 agaagtgggt cgaagaaccc ctctccgacg ttctcgtgcg cgccaatccg tttcagcgtg
      421 agtttctcca gcggctgttg gccgaagttc ccgcgctcgc gcaacaattg gtcttgcgcc
      481 gtgtgcgaag acggtttcat cctgatgttg aactttattc agagcctgac aacggaataa
      541 gcgccgattt cgagcatgaa cgcagccgct ggggattctc tatcgatgcc gaaaccaatg
      601 tcatctgcgt ctggaacatg aatggccagg gagaatacgc cgactggaac gggaccggac
      661 gagattctca aggagatgcg attcggcaga ttcgcgaatt catcgccagg gatccctgaa
      721 ttatgctcag tagatgccct cctccgctcg tcagccgtgc gaatcgggct tgcggattcg
      781 catcgcgccc gaagaagtct cgttcaccga ctggcctctg cgccagcggc cgggcggctc
      841 gctcctcgcg ctggcgctgg ccgggggagt cagttggctc gtcggctggg cggcggggcg
      901 ctgggaagtg ggcgcgatcg cggcagccgc actggctttc accttgtggc aaatctggct
      961 gccggtgcgg tacgaaatcg acggccgcgg cattgcgcag ttgtacttcg gacgctggcg
     1021 gcggcggata ccgtggtcgg caatccgaca gtacgaagtc cggtcggatg gggttttgct
     1081 ggttcccgat gcagccgtca cgccgctttc gccgcttcgc ggcctctact tgcactgggg
     1141 agcgcaccgc gaggaagtgc tcgccaacct cgagtattac gtccatagtt ggcgatcacc
     1201 ggccggcaac tcgatctacc ggcggccgtc gccgtaatgg ggtcgggagt caattgcggc
     1261 tggcatcttc cattatggaa gcccgttgtc gcaattgact cccgacccct tctgggtcgc
     1321 caccagcagc agatcgacgc ccagcggctc gctgctgaat tccacgcatt cggccggcaa
     1381 ccccgccgcc gcagccaagt gcgcaagctc gtcggatgtg cgataggtga gataccagtt
     1441 gcccagccat tccatatagg tccgcgttgg attatgcggt gcgaattgaa acacggccac
     1501 gcggccgccg ggcgcaagcc gctcccaaaa cgtccgcaac atcgcggcgg ccgccgcctt
     1561 gtccagatag tcgaagagtc ccgggcacag cagcagatcg gactcgttca atggcgccgc
     1621 cagcgccggc cggtccggca ggcgaaacag gttggcgctg actgccgtca gccgatcttc
     1681 gccgatcagc ccctgaagac tatcgcgagc atattcgatg gccgacggat cgagatccag
     1741 tagtgtgacc gacagccttt ggcgctccga ttcgtccagc cgcatgagcg catcgcgaac
     1801 ttccagccca aacgccgaac cgacaacagc gatccttgct cccctcgccc cttgtgggag
     1861 agaggtcggg ggtgaggggg agttgcgcac caggtccaca atccactccg tcatcatcgc
     1921 catccgatgc cgcacggccc gcggcgccgc ttcctcctga aaataccggt cgaacagccg
     1981 gcccagagga tcctcgcaca ggcggttctc gtagatccgg gcgagcatct cgaaatcgcc
     2041 cgcgtagccg cgcggcttgg tgcgggcctg gttctgcagc cagccgcgct ggagcacatc
     2101 gccggcgacg ttccacagct ccgacgaggg caggcgatta tccggcccgc gaaggcccag
     2161 tgaatcgagc cccgccaggc actcgtccag gacggctttg accgccgcgt aatgctgctg
     2221 caggtctccg ccagcgagat cgccttcaat cgccttcaga ttctggagca gctgctcggc
     2281 ggaatggcgg acgaactggc gaatcgctgg gctcatacga cgaaggcatg tcgcgcttgc
     2341 aaaccgcaat tcccgtcagt ctaattgggg tcgcttctcc ctcactttac cgaattctca
     2401 tgcctgtcgt cgttgcgtgc cgctgtggac agcgattcgc tgcccaggat cacctgtttg
     2461 gcacgcaggt cgcctgcccg gcatgtggca gtccgctcgt catcccgcat ccgcagatgc
     2521 agccggcgcc aatgcccgcc ccggcgatgc ctgcgccagc ttctgcgccc ttacgcggcg
     2581 cgtacccggc gccgcagccg cagaacgccc cggcgcccgc gagcggagga gcatccagcg
     2641 actggaagct gatcggcttg atcctggggg gcgcgggatt gctggccgtg gtcgtcgtga
     2701 ttggcctggc gatcgccttc agcgggccgc cgagaaaccc cgcggccctg gccaatgtgc
     2761 cggctggtgc gggtccgccg cccagctccg ccccgccgcc tccaccgagt ggatcggcac
     2821 tgcccccgac tcctaccgcg gccccgccgg gaagctccgc ggccggcgcc gcgacaacgc
     2881 ccgccaattc caatcccttc gccgctgccg cggcctccac atcatccacc tcttccccag
     2941 agtcatcgaa ttccaggccc gcggccagcc aatcctccag tgcttccacc cagccgccgc
     3001 gctccgcctt tcgcgacgat cctacggccg ccggccaagg catcgccttt ctgccgacgg
     3061 ccgtcgattc gtggcacggc gaccagaccg cccgccgcgg actccttcgc gtgggcgatt
     3121 ccgagccggc cttccagcac ttcagttgga tgacgcagct gctgccgttc ctcggccatc
     3181 agaaggaata cgaccggatc gatttcagga aggagctgtt caccgaaacg aacctggagg
     3241 tcggcttgga ggtgattccg gcgtttcaga atccgctcga cgaccgcaag acgtggaagg
     3301 gccatccgtt tcaaggcctg gccctgacgc actttgccgg catgtccggc atcgaagata
     3361 cccgcaacgt cgtggccgcc acgctgccgc gcagcgatcc ccgggccggc gtttttggct
     3421 atgacgaagt ggcccgtccc gagcagatca ccgacggtac cagccagacg atcatgatcg
     3481 ttgggaccgg ggcgctggcc aatccgtgga tcatgggggg cggcggcacg attcgcgggg
     3541 ctcgcgagcc ctatttcgat cccatctccg gcctgggcac gcgcaatttg ccgcaagccg
     3601 gcacgctggc cgtcatggcc gacggcagcg tgcggttcat ttcggccagt gtcgatccgc
     3661 aggcttttcg agccatgtgt acgatccatg gccgcgacac ggtcgatctg gagcgtgccg
     3721 cccagccgtt cctgctcgat agtattcaga aatagtgaca gttcggagga gtcccatgcg
     3781 gcaccaaatc gctttggcgc tcgtcatctt tggtttcgcc gcaagcccgg ccgccgccga
     3841 cgtcgtcttc taccgactgc cgaccagcag cggcagcgtc gtcgtcctgg agggttcggc
     3901 gacggtcaac cccggcggga cggtcacgtt cagccacaat cgctttggca agattcactt
     3961 cgatttggaa aacacggaaa tccgcaaggc cccaaccgtc cgcgagcagt tttcccgcca
     4021 actgaaccgc gccggaaacg acgccaacaa gctgctggag gccgcccact gggcattgcg
     4081 gcacggcctc ctgccgcagt tctacagcgc cgtcgata
//
LOCUS       DASUGB010000147         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1824029, whole genome shotgun sequence.
ACCESSION   DASUGB010000147 DASUGB010000000
VERSION     DASUGB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824029"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFV87_02920"
     CDS             complement(<1..196)
                     /locus_tag="VFV87_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102733.1"
                     /translation="MNAWQDILAAALVLAAGGFVAWRAWLAVAARPGGGCASDCGTCR
                     SAAAKPVLQIEGERTDGRRCP"
     gene            complement(193..2400)
                     /gene="feoB"
                     /locus_tag="VFV87_02925"
     CDS             complement(193..2400)
                     /gene="feoB"
                     /locus_tag="VFV87_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX5102734.1"
                     /translation="MSVASPPRPLTVALIGNPNTGKSTLFSGLSGLRQRVGTYPGVTV
                     EKKIGRMEIQGRPILLVDLPGTYSLAPRSPDEMVAVEVLLGRRGDVAAIDVVLCIVDA
                     SNLERNLYLVSQVFELGLPTVVALNMVDVAKEQGLSIDLDRLRKQLPAPVVAVQANRG
                     IGLHELKRELLLAADRQTPVRTSPFPEPFCREVERLCRRDCANGHTPLPRYLLERLLL
                     DTSGYVARSGLPGIDEPMLAEVKAARSRLAAQGLAVPGVEAVARYGWAGEVCAGVIQR
                     PVERPRTLSDRLDRALTHRVWGTLVFLLVMLLMFQAVYYVPDVTRATWALDQIKGLAA
                     DGIARLVPEGAFRSLLVTGVIEGVGGVVVFLPQIFTLFFFIAVLEDCGYMARAAYLMD
                     KLMSRIGLSGKSFIPLLSSFACAVPGIMATRVIENRRDRLVTILIAPLMSCSARLPVY
                     ALLIAAFIPNRSYLGGLLTLHGLVLLAMYSLGIVAGIAVAWILKRTILRGPTPAFVME
                     LPSYKLPGLRVVLHRMLEQGWAFIYRAGTLILAVSVLVWGLAYYPHDESLLPHELLAA
                     RATLTQQAASGRPVVEELAHVENQIQGAHLEASYLGRMGKLVEPLVRPLGWDWRIGCA
                     AIASFPAREVVIATLGVIYNLGEGQDEESGELSATLRAATWQETDRPVFTIPVALSVM
                     VFFALCAQCASTLAIMRRETGSWRWPAFTFAYMTMLAYLGAFLTFQVGTWIGL"
     gene            complement(2563..2691)
                     /locus_tag="VFV87_02930"
     CDS             complement(2563..2691)
                     /locus_tag="VFV87_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HEX5102735.1"
                     /translation="MPGVELSVIGVAPLGDPLELEVRGYRLSLRKSEAGRVQINDQ"
BASE COUNT          534 a          912 c          830 g          421 t
ORIGIN      
        1 aggggcacct tcgcccatcc gttcgttcac cctcgatttg gagcaccggc ttggccgcgg
       61 ccgagcggca tgttccgcag tcgctggcgc agccgccgcc tggccgggct gccaccgcca
      121 gccaggctcg ccaggcgaca aatccgccgg ccgccagcac gagggcggcc gccaggatat
      181 cctgccaagc gttcataagc cgatccaagt ccccacctga aacgtcagga aagcccccag
      241 atacgccagc atcgtcatat aggcaaacgt gaacgccggc cagcgccagc tgcccgtttc
      301 gcggcgcatg atcgccagcg tcgaggcgca ctgggcgcag agcgcgaaga ataccatcac
      361 tgaaagggcc acggggatcg tgaacacggg ccggtcggtc tcctgccacg tcgccgcccg
      421 cagcgtcgcg ctgagctcgc cggattcttc gtcctgccct tcgcccaggt tataaatcac
      481 gccgagcgtc gcaatgacga cttcgcgggc tggaaacgac gcgatcgccg cacagccgat
      541 ccgccagtcc catcccaaag ggcggaccag gggctcgacc aacttgccca tgcggcccag
      601 gtaactcgcc tccaggtgag cgccctggat ttggttctcg acgtgtgcca gctcctcaac
      661 gacgggcctg ccgctggctg cctgctgggt gagggtggct cgcgcggcga gcagttcgtg
      721 cggcaggagc gactcgtcgt ggggataata cgccagcccc cagacgagga ccgatacggc
      781 caggatcagc gtgccggccc gatagatgaa ggcccaacct tgctccagca tgcggtgcag
      841 caccacccgc agaccgggca gcttgtaact gggcaattcc atgacgaacg ccggtgtagg
      901 tccgcgcagg atggtccgct tcaagatcca ggccactgca attcccgcca cgatgcccag
      961 cgagtacatc gccaggagca ccaggccgtg gagcgtcagc aatccgccca ggtacgagcg
     1021 attgggaatg aacgcggcga tcagcaaggc atagaccggc agacgggccg agcagctcat
     1081 cagtggggca ataaggatcg tcacgaggcg gtcgcggcga ttctcgatca cgcgcgtcgc
     1141 catgatgccc ggcaccgcac aggcgaatga cgagaggaga ggaatgaacg acttgcccga
     1201 caagccgatc cgtgacatca gcttgtccat cagatacgcc gcccgcgcca tgtaaccgca
     1261 atcttcgagc acggcgatga agaagaatag cgtgaagatt tggggcaaga acacgacgac
     1321 gccgcccaca ccttcaatga cgccggtgac gagcagcgag cggaatgccc cctccggcac
     1381 aaggcgggcg atgccgtccg ccgccaggcc tttgatttga tcgagggccc aggtggcccg
     1441 cgtcacgtcg ggcacataat agaccgcctg gaacataagc agcatgacga gcagaaacac
     1501 cagcgtgccc cacacgcgat gagtcagcgc gcgatccagg cgatcgctta gggtccgtgg
     1561 tcgctcgaca ggccgttgaa tcacccccgc gcacacctca cccgcccaac catagcgggc
     1621 cacggcttcc acgcccggca ccgccaggcc ctgggccgcc agccgcgacc gggcggcttt
     1681 cacctcggcc aacatcggct cgtcgattcc cggcaggccc gaccgggcga catagccgct
     1741 cgtatcgagg agcaggcgct cgagcaggta tcgcggcagc ggcgtgtgac cgttggcaca
     1801 gtcgcgccga catagccttt cgacttcccg gcaaaagggc tcgggaaagg ggctcgtgcg
     1861 gaccggcgtc tggcgatcgg cggccaacag cagctcgcgc ttcagctcgt ggaggccgat
     1921 gccccgattg gcttgcacgg ccacgaccgg cgccggcaat tgcttccgca ggcgatcgag
     1981 atcgatgctc agcccctgct ccttggccac atcgaccatg ttgagcgcca cgaccgtcgg
     2041 cagccccagc tcgaacacct ggctcaccag atacaggtta cgctcgaggt tgctggcatc
     2101 gacgatgcac agcaccacgt cgatggcggc cacgtcgccg cggcgcccga gcagcacctc
     2161 gaccgccacc atctcgtccg gcgagcgcgg ggccaggctg tatgtgccag gcagatcgac
     2221 caagagaatt ggccgcccct gaatctccat gcggccgatt ttcttctcga ccgtaacgcc
     2281 cggataagtc cccacccgct gccgcaggcc cgacaggccg ctgaacagcg tcgatttgcc
     2341 cgtattggga ttgccgatca aggcaaccgt cagcgggcga ggcggggacg cgacggacat
     2401 gggtatttgt aaatgaccga gcaccaatga ccaaggtccg cacaaattcg aatcagcaat
     2461 tcacaaaatg ttccaaacgg tgcgggtcca attcgagcat tgctgcttgg tgcttgtttg
     2521 gtaattggtg cttggtcgtt ggagcttcgt tttcggtgct tgttattggt cattgatctg
     2581 gacgcggcct gcttcgctct tgcgcaagct cagccggtag ccgcggactt ccaactccag
     2641 cggatctccc agcggcgcca caccgatgac agacaactcc acgccgggca ccaggcc
//
LOCUS       DASUGB010000148         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1267453, whole genome shotgun sequence.
ACCESSION   DASUGB010000148 DASUGB010000000
VERSION     DASUGB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267453"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1652
                     /locus_tag="VFV87_02935"
     CDS             252..1652
                     /locus_tag="VFV87_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102736.1"
                     /translation="MPKSSAADNRFVCTLLLSWALLTVGHTASGQTTDAKIRPAKPKP
                     GRSLAERYPLPYPPRLPEGKMVVTESDPSFLVPNDKLREGIEIAKTPPTVDFAFYPEQ
                     NYPGNPWSHRSDGIVVGDKYYSSSNDHLAPRGTAHLWEYNAASKQFRLLCDTTKFLES
                     QKAFPPEMDYRPGEMQSRIDVGSDGWLYYATDRGSPTITDDAHGYRGEWILRTNPQTR
                     QTQIVATHPVARHTLPASVLDPQRMIYYGGTAPGKDAANQKVQFFALDVKTGKLLIQD
                     DDGPTRTLAFSPSTGRVFWEGKMYDPATNQITPLDMPHVRSASRETPQGIIYGTSERS
                     ADLWALDVKTRRVKQLGSGAVATQEYVASVEADPTGRYLYYVPGAHGGAAGDGTPVVQ
                     YDLKTGKRKVLAFLYQHFWDKYSYALDGSFGSALDEKGERFFISWDGWRKGQPRGWES
                     AAITVLHIPASERPVD"
     gene            complement(1724..2674)
                     /locus_tag="VFV87_02940"
     CDS             complement(1724..2674)
                     /locus_tag="VFV87_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102737.1"
                     /translation="MLERRLCLACDIGFEDGQLVVSGDKADNVVAITADGAGGVTVSC
                     DDGEEQSFEGVEGITVDLDQGNDSATIDLSAGGFSSLSVSVEGGKGDDAVSVNLGDLT
                     DAEIDVAVEGGKGNDSVLATVGAVAGSQVELEIEAGPDDDIISAAIGAVTDSGVELSV
                     EGGPGDDEVILDLLGAIAGESDVEVSVEGGPGDESIFLNAIAVDIAEAASLAVSVEGG
                     PGDDFVNIDYEGVVAGALSVETELGPGADESAINLTAAAGSTGAIEAHLEGGPGDDTL
                     TLNVNTDEADDVEVDASLAGGPGFDTCVATDNVEITGCEA"
     gene            complement(2925..>3794)
                     /locus_tag="VFV87_02945"
     CDS             complement(2925..>3794)
                     /locus_tag="VFV87_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate carboxy-lyase"
                     /protein_id="HEX5102738.1"
                     /translation="VFGQLIHELTGDVIPTVLHGVKAVHAVDAAGVHPLLLAIGSERY
                     MPFHENPRPQELLTQANAILGQGQLSLAKFLFIVNRFDDEQLDVHDIPAFFRHVLSRV
                     DWRRDLHFQTNTTIDTLDYSGDGLNMGSKVVIAAVGKPIRQLATEIPSDLRLPEGFRE
                     PRRALSGVLVVQGPKSKVQSSDASTLDLGPWTLDFSPPDFPLIVVADDSDFTSAALAN
                     FLWVTFTRANPAADIHGVGEFTDRKHWGCTGPLVIDARIKPHHAPPLVEDPEVTKKID
                     ALAARGGPLARWL"
BASE COUNT          699 a         1231 c         1198 g          666 t
ORIGIN      
        1 catcggcatc cgacagcctc agttcccgct agctgccatc ggaggctttg atctcgcgtt
       61 gcaccgtcga agtcgcttca cccagcaggc aagttgtagc gtaagtacag caactgtcaa
      121 gacgaccact atatcgtgtg agccgcgggg ctgtacacca ctaccagtca agggtcgtgc
      181 ccgattgacg atcgatggaa cgcgccgcga tcgcctggtc ctcgcgctga acgcggacgg
      241 aagcagaaca catgccaaaa tcctcggctg ccgacaatcg atttgtctgt accttgttgc
      301 tctcttgggc gctgctcacg gttggacata cggcttcggg ccaaacaacc gacgccaaaa
      361 tcaggccggc caaaccgaag cccggacgct cgctggccga gcggtatccg ctgccgtatc
      421 cgccgcggct gccggagggg aagatggtgg tgacggagag cgatccgtcg tttctcgtgc
      481 cgaatgacaa gctgcgcgag ggcatcgaga tcgcgaagac gccgccaacg gtcgatttcg
      541 ccttctaccc ggagcagaac tatccgggca atccgtggtc gcaccggtcc gatgggattg
      601 tcgtcggcga caagtactac agctcgtcga acgaccatct cgccccgcgc ggtacggccc
      661 atttgtggga atacaacgcg gcgtcgaagc agtttcgact gctctgcgat acgacgaagt
      721 ttctcgaatc gcaaaaggcc tttccgccgg aaatggacta tcgccctggc gaaatgcaaa
      781 gccgtatcga cgtcggcagt gacggctggc tgtattacgc caccgaccgc ggctcgccga
      841 cgatcaccga cgacgcacac ggctaccggg gcgagtggat cttgcggacg aacccgcaga
      901 cgcgccaaac gcagatcgtc gccacacatc cggtcgccag gcacacgctg ccggccagcg
      961 tcctcgatcc ccagcggatg atctactacg gcggcactgc tccaggcaag gacgctgcga
     1021 accagaaggt gcagttcttt gccctcgatg tgaagaccgg caagctgctg atccaggacg
     1081 acgacgggcc gacgcggacg ctggcgtttt cgccctccac tggccgcgtg ttctgggaag
     1141 gcaagatgta cgatccggcc acgaaccaga tcacgccgct cgatatgccg cacgtccgct
     1201 ctgcctcgcg agaaacgccg cagggcatca tctacggcac ttccgagcga tcggccgatc
     1261 tttgggcgct cgatgtgaag acccgccggg tgaagcaact cggcagcggc gcggtcgcca
     1321 cgcaggagta cgtcgcctcg gtcgaagccg acccgactgg ccgctacctg tactacgttc
     1381 ccggcgctca cggcggcgcg gcgggcgacg gcacaccggt cgtgcaatac gacctgaaga
     1441 caggcaaacg aaaggtcctc gcgttcctct atcagcactt ctgggacaag tacagctacg
     1501 cgctggatgg ctcattcggc tcggcgctcg acgagaaggg cgagaggttc ttcatctcct
     1561 gggacggctg gcgaaagggc cagccccgcg gctgggaatc ggccgcgatc accgtgctgc
     1621 acattccggc ttcggaacgg cccgtggatt gagagcccga gcccagggcg ggcctgagct
     1681 cgggcaagga cgcatgttgt ccaacagatc cagccgtgtc ggcttatgcc tcgcagcccg
     1741 tgatttccac gttatccgtg gcgacgcacg tatcgaagcc gggaccgcct gccagcgagg
     1801 catcgacttc cacgtcgtcg gcttcgtcgg tgttgacgtt cagcgtcaga gtgtcgtcgc
     1861 cagggccgcc ttccagatga gcctcaatcg cgcccgtgct gccggcagcc gcggtcagat
     1921 tgatcgctga ctcatcggcg cccgggccca actccgtctc gacgctgaga gcgccggcaa
     1981 caaccccctc gtagtcgata ttgacgaaat cgtcaccggg gccgccttca accgacacag
     2041 ccagcgaggc agcttcggcg atatcgaccg cgatggcgtt gaggaagatc gattcgtctc
     2101 cgggaccgcc ttccacgctg acctcgacgt cgctttcgcc cgcgatcgcg ccgaggaggt
     2161 ccagaatcac ttcgtcgtcg cccggaccgc cttcgacact cagctcgacc ccgctatcgg
     2221 tgaccgcacc gatggccgcg ctgatgatgt cgtcgtccgg tccggcttcg atctccaact
     2281 cgacttgcga gccggcgacc gcgccgacgg ttgccaggac agaatcgttc cccttgccac
     2341 cctcgaccgc cacgtcgatt tcggcgtcgg tgaggtcgcc caggttgacg ctgaccgcgt
     2401 cgtctccctt gcccccttcg accgacaccg acaggctgct gaatcctccc gcggacagat
     2461 cgatggtcgc ggagtcgttc ccctggtcca gatcgacggt gatgccttcg acgccttcga
     2521 aggactgctc ttccccgtcg tcgcagctga ccgtgacgcc tcccgccccg tcagccgtga
     2581 tcgcgacaac gttgtccgcc ttgtcgccgc tcacgacaag ctgtccatcc tcgaagccga
     2641 tgtcgcacgc cagacagagg cggcgctcga gcatttcgag tcggggcgcg aaagtccgca
     2701 accctgtcct gctctgcttc cggaaaaatg cctgcatgtt gccctccttg aagggaacgg
     2761 gtaatgaagc cctggctgca gcgggcatag cgccgcaggg gacgccgcct tgcctgcaag
     2821 ctgcaggccg cagtcattac ggcgacaagc cgctcggctg tacccgcgga acgagtgaac
     2881 gagttccccc gacaggttgt cgctagcgag gaaggctcgg ttagctatag ccaccgggcc
     2941 agcgggccgc cgcgggcggc gagggcgtcg atcttcttgg tgacttcggg gtcttcgacg
     3001 agcggcgggg cgtggtgcgg cttgatgcgg gcgtcgatga cgagcgggcc ggtgcagccc
     3061 cagtgcttgc ggtcggtgaa ttccccgacg ccgtggatat ccgccgccgg gttggcccgc
     3121 gtaaacgtga cccaaaggaa gttggccagc gcggctgagg tgaagtcgga gtcgtcggcg
     3181 acgacgatga gagggaagtc gggcggggag aagtccaaag tccaaggtcc aaggtccaag
     3241 gtcgatgcat ctgaactttg gactttggac tttggacctt ggactacaag gacgcccgac
     3301 agcgcccgcc gcggttcgcg aaaaccctcc ggcagccgta aatccgacgg gatttccgtc
     3361 gccaactgtc gaatcggctt gccgacggcg gcgatgacga ctttcgagcc catgttgagc
     3421 ccgtcgcccg aatagtcgag cgtgtcgatc gtggtgttcg tctggaagtg caggtcgcgc
     3481 cgccaatcga cgcgcgacag cacgtggcgg aagaacgccg ggatgtcgtg aacgtcgagc
     3541 tgctcgtcgt cgaagcggtt aacgatgaac aggaacttgg cgagcgagag ctggccttgg
     3601 ccgaggatcg cattggcttg cgtgagcaat tcctgcggcc gcgggttctc gtggaacggc
     3661 atgtaccgct cgctgccgat cgcgagcagg agcggatgca ctccagccgc atcgacggcg
     3721 tggaccgcct tcacgccgtg cagcacggtc gggatcacat cgccggtcag ctcgtgaatg
     3781 agctggccga agac
//
LOCUS       DASUGB010000149         6059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1963303, whole genome shotgun sequence.
ACCESSION   DASUGB010000149 DASUGB010000000
VERSION     DASUGB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963303"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1903)
                     /locus_tag="VFV87_02950"
     CDS             complement(356..1903)
                     /locus_tag="VFV87_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102739.1"
                     /translation="MAEPFDPYRKWLGIPSREQPPHHYRLLGIALFEDDPDVIENAAT
                     RQMTHVRTFQTSKQHGPLSQRILTELSAAKLCLLTPESKAEYDAQLRAQFEAAGEHTA
                     AETTAEAGEGDAGGGDAEVELPPFASSRGSEVRWRTGAAPEIAIDPSPVPIPMPQIVT
                     AAAAAPAFPAVRSTTKMAAAARPTQKRSVLPIALIAAGLLGLVALVSVAVAIANHLGS
                     DHARLPVHTRPASANGERNPASAFPEGKPASKSSQSSDSLGTPFPVGGSTPAGGGIAT
                     ARPATPFPVPAPTPADDPPAPQAISQADRDELVDRMRQSLFRSEQSLRGRENEEFQRQ
                     FAMAEELAASKELPDSAKFQEQVDDLRVLKKLNDEFWTTVQNNLRNKLAVGEKIEFHQ
                     HKFELLARDGERVEYLLNGEQHADAIGQLEPKAAILIASKSVKWEDPVTFLPIAAFLS
                     VDGKVTDEAERKFGKQLFAIGRLIGQANPAIARKLGLRDVNEEVKLDDLVEQIPLPLL
                     QPEPKPE"
     gene            2209..3120
                     /gene="fhcD"
                     /locus_tag="VFV87_02955"
     CDS             2209..3120
                     /gene="fhcD"
                     /locus_tag="VFV87_02955"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HEX5102740.1"
                     /translation="MQLGETLIEDTFAEAFGMRYARLIVTAADEYWLDAALRELTGYS
                     SSVIACDAEAGVERRLSPLDTPDGRPGASVLVFGFSTDALNKSVPNRVGQCVMTCPTT
                     AVYNGLPESETRIPLGKKIRLFGDGYQKSKQVGGRRYWRIPVMDGEFVVEDVVGAEKG
                     VAGGNIILQSVDQAAGLAAVRRATEAIWKLPGVITPFPGGSARSGSKVGSRYKGLVAS
                     TSDAYCPTLRGRVESKLHPQANCAYEIVIDGISEAAVAAAMTAALRAAAGPGVVAITA
                     GNYGGKLGKFHFHLHKLLDGSATGANS"
     gene            complement(3128..5821)
                     /gene="topA"
                     /locus_tag="VFV87_02960"
     CDS             complement(3128..5821)
                     /gene="topA"
                     /locus_tag="VFV87_02960"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5102741.1"
                     /translation="MAKRAAKSPATGKSLVIVESPAKAKTISKFLGGGYTVEASIGHI
                     RDLPQGAKEVPAQYKGQPWARLGVNVEDNFTPIYVVPPEKKSQVAKLRDLLKGSSQLF
                     LATDEDREGEAISWHLCEVLKPKVPVRRLVFHEITKEAIQDALANPRDIDDGLVRAQE
                     TRRILDRLYGYEVSPLLWKKVRTGLSAGRVQSVAVRLIVERERQRIAFRTATWWDLIA
                     RLAAEGGKEFDAELVSVGGRKIPSGKDFDAATGKIKDPQHLLLDEQKAHELAERLKRG
                     QFRVKSVEQRPYTRKPPEPFTTSTLQQEANRKLNFGARRTMNAAQSLYQNGHITYMRT
                     DSTSLAQVAIDAARDLVRAEYGGEYLPQSPRIYKSKVKNAQEAHEAIRPAGHPFELPS
                     VLKGQLPDDEYRLFELIWKRTIASQMVDARGQIITVTIEVDDAVFQVTGKTIEFAGFL
                     RAYVEGSDDPEAELADQERILPAMKTGDPLACRELTPKDHTTQPPARYSEASLTAALE
                     ERGIGRPSTYASIIETILARQYVFKKGNALVPTWTAFSVAKLLEQHLPDLVDYEFTAQ
                     MEDLLDAISRQEHDSLTYLSDFYFGKKNSQEHPGLKQQLDSKLGEIDARDMSRFPLGK
                     PAEGPHREEVAVRVGKFGPFIEQGERKASIPDDLPPDELTLAKALEMLDQASVGDEPL
                     GIHPDTQRPVYLKTGRFGPYVQMPADDEDEKPKNASLLSGMKPEDVTLEVAIKLLSLP
                     RTLGDHPESREPVVALSGKFGPYVKCGSETRSLPAEVSPIDVTFEQALELLAQPKQRG
                     RGRAAPREPLKTFDASPITSQPVKLLSGRYGNYVTDGTTNATLPKDIDPETLTFQQAL
                     DLLAERAARGPAAPRGRKGRAKPAAPKKAAKKSGRAKSATE"
BASE COUNT          941 a         1981 c         1967 g         1170 t
ORIGIN      
        1 gctgcttcag ccgacttagc tatcgccgtg ctggatgcca acacagcgta ccaagagaat
       61 gggatgagga gcccccgccc gctgattgtc cacaacaagt gcgatctgtg cccgccgccg
      121 agcgacggtc gtccgccggg actgcacgtc agcgcgaagt gggggctagg aatcgatcag
      181 ttaatgcggg ccatcagcct tcacttggcg ccgaatcctc ctccccgcgg cgcggcggtg
      241 ccgttcacgg ccgcacaact cgcggccatt gaccgcgcca aagcgcagct gacaagaggc
      301 gagccctgcg cagcgcgcga cgagcttgtt gtgcaaccat tcccttccgc tgcctctact
      361 ctggcttcgg ctccggctgc aagagcggca gaggaatctg ctcgaccaga tcgtcgagct
      421 tgacctcctc gttcacgtcc ctcaagccca acttgcgggc gatcgcggga ttggcctggc
      481 cgatcagacg gccgatcgca aagagctgct tgccgaattt ccgctcggcc tcgtcggtca
      541 cctttccatc gaccgacaag aacgcggcga tcgggaggaa ggtcaccgga tcttcccact
      601 tgaccgactt cgacgcgatg aggatcgccg ctttgggttc gagctggccg atcgcgtccg
      661 catgttgctc gccgttgagc aggtattcga cccgttcgcc atcgcgcgca agcagttcga
      721 acttgtgctg gtggaattcg atcttctctc ccaccgcgag cttgttccgc aggttgttct
      781 gcacggtagt ccagaattcg tcgttcagct tcttgagcac ccgcaggtcg tccacttgct
      841 cctgaaattt ggctgagtcc ggcaattctt tgctggccgc gagctcctcc gccatcgcga
      901 attgccgctg gaattcttca ttctcgcgcc cccggagcga ttgttcgctc cgaaagagcg
      961 attgtcgcat tcgatcgacg agctcgtcgc ggtcggcctg gctgatggcc tgcggtgcgg
     1021 gcggatcgtc cgcgggagtc ggcgccggga cggggaaggg tgtcgctgga cgggccgtgg
     1081 caattccgcc gccggctgga gtgctgccgc ccacgggaaa aggcgtgccc aggctgtcgc
     1141 ttgactggga cgacttgctc gcgggcttgc cctccggaaa cgcactggcc ggattccgct
     1201 cgccattggc tgatgcgggc cgcgtgtgaa ccggaaggcg cgcgtggtcg cttcctaaat
     1261 ggttggcgat cgccaccgcg acactgacca gcgccaccag gccgagcaaa ccggccgcaa
     1321 tcagcgcgat cggcagcacg gacctttttt gggtgggccg cgctgccgcc gccatcttcg
     1381 tcgtgctgcg gactgccgga aacgccggtg cagcggccgc tgccgttacg atctgcggca
     1441 tgggaatggg aaccggcgac ggatcgatcg ctatttccgg tgcggcgccc gttcgccagc
     1501 ggacctcgct gccgcggctg gacgcgaaag gcggcaactc gacctccgcg tcgccaccgc
     1561 ccgcatcgcc ttcacccgcc tcagcggtcg tttccgccgc ggtatgctcg cctgccgctt
     1621 caaactgcgc ccgcaactgg gcgtcatact ccgccttgct ctccggcgtg agcaggcaca
     1681 gcttggccgc cgacagctcg gtcagaatcc gctgcgagag cggcccgtgc tgcttgctcg
     1741 tctggaacgt ccgcacgtgc gtcatctgcc gcgtcgccgc gttctcgatc acgtccgggt
     1801 cgtcttcaaa cagggcgatc cccagcagcc gatagtggtg cggcggctgc tccctggacg
     1861 ggatgcccag ccatttgcgg tacgggtcga aaggttcggc cattgggccg tcggctttcc
     1921 ggatggggga ttgcggtcaa ggcgcaacac ctcattatcg ctcggctggc cacaggcgac
     1981 aaccgcgatt gcggccgggg ttggcgggaa tcgaatggcg caaagcattg gctggtgcga
     2041 tcttgcgcat caatccgcct cacgccggcc gtaggttgcc acgtgaatat ggctccaccc
     2101 ccgatccgac gttaccggga atccggccga agcagcgcgc ccttggcagc gcggcgctcg
     2161 aaggtcatca ttgcaacaaa cctttgcagc cccccagccg agatcgacgt gcagcttggc
     2221 gagaccctca tcgaagacac ctttgccgag gccttcggca tgcgctacgc gcgtctgatc
     2281 gtcacggccg cggacgaata ctggctcgac gcggccctcc gcgagttgac gggctacagt
     2341 tcgtcggtga tcgcctgcga tgcggaggcc ggcgtggaaa ggcgtttgtc cccgctcgac
     2401 acgccggatg gccgaccggg agcgagcgtc cttgtctttg gcttctcgac cgacgcgctc
     2461 aataagagcg tgcccaatcg cgtcggccag tgcgtcatga cctgccccac gaccgcggtc
     2521 tataacggcc tgccggaatc cgaaacgaga atcccgctcg gcaagaagat ccgccttttc
     2581 ggcgacggct atcagaagag caagcaggtc ggcggacgcc gctactggcg gattcccgtg
     2641 atggacggcg aattcgtcgt cgaagacgtc gtcggagcgg agaaaggagt cgcgggtggc
     2701 aatatcattc tgcaatcggt cgatcaagca gcgggattgg ccgcggtccg ccgggcgacg
     2761 gaggccattt ggaagctgcc gggcgtgatc acgccgtttc ccggcggctc agcccgcagc
     2821 ggcagcaagg tcggctcgcg ttataagggg ctggtcgcct cgacctccga cgcgtattgt
     2881 cccacgctcc gtggccgagt cgaaagcaag ctgcacccgc aagccaactg cgcctacgag
     2941 atcgtcatcg acggcatcag cgaagccgcc gtcgccgccg cgatgaccgc cgccctccgc
     3001 gcggctgccg gccccggcgt tgtcgccatc accgccggca actacggcgg caagctcggc
     3061 aaatttcact tccatctgca caagctgttg gatggctctg caactggcgc aaactcctga
     3121 cgcgtggcta ctccgtcgcg gatttggccc ggccggactt cttggcagct ttcttgggag
     3181 ctgcaggttt ggcgcggccc tttcggccgc gcggagctgc ggggccgcgg gcggcgcgct
     3241 cggctaaaag gtcgagggct tgctggaagg tgagcgtctc ggggtcgatg tccttgggga
     3301 gggtggcgtt cgtggtgccg tcggtcacgt aattgccgta gcggcccgag aggagcttca
     3361 ccggctgcga ggtgatgggc gaggcatcga acgtcttgag cggctcgcgc ggggcggccc
     3421 ggccacggcc gcgctgcttc ggctgggcca gcagttccag ggcctgctcg aacgtgacgt
     3481 cgatcggcga aacctccgcc ggcagcgacc gcgtctcgct gccgcacttg acgtacggcc
     3541 cgaatttgcc gctcaaagcg acgaccggct ctctcgattc cgggtgatcg ccgagcgtcc
     3601 gcggcagcga cagcagcttg attgccacct ccagcgtcac atcctccggc ttcatccccg
     3661 acaagagcga cgcattcttc ggcttctcgt cttcgtcatc cgccggcatc tgcacgtacg
     3721 gaccaaagcg ccccgttttc aagtacacag gccgctgggt atcggggtga atgcccagcg
     3781 gctcatcacc cacgctcgcc tggtcgagca tttccagggc cttggccagc gtcagctcat
     3841 cgggcggcag atcatcggga atgctcgcct tccgttcgcc ctgctcgata aacggcccga
     3901 acttgccgac gcggaccgcg acttcttcgc ggtgcggccc ctccgccggc ttgcccagcg
     3961 gaaagcggct catgtcgcgg gcgtcgattt cgcccagctt gctgtcgagc tgctgcttca
     4021 gccccggatg ctcctggctg ttcttcttgc cgaagtagaa atcggacaag tacgtgaggc
     4081 tgtcgtgctc ctgccggctg atcgcgtcga gcaggtcttc catctgggcg gtgaactcgt
     4141 aatcgaccag gtccggcaga tgctgctcca gcagcttggc gaccgaaaag gcggtccaag
     4201 tcggcaccag ggcgttgcct ttcttaaaga cgtactgccg ggcgagaatc gtttcgatga
     4261 tcgaggcgta cgtgctcggc cggccgatgc cgcgctcttc cagggccgcg gtgagcgagg
     4321 cttcgctgta gcgggcggga ggctgcgtcg tgtgatcctt gggcgtcagc tcgcggcagg
     4381 cgagggggtc gcccgtcttc atcgccggca gaatccgctc ctgatcggcc aactcggcct
     4441 cgggatcgtc ggagccctcg acgtacgccc gcaaaaagcc ggcgaactcg atcgtcttgc
     4501 cagtgacctg aaacacggcg tcatcgacct cgatcgtgac ggtgatgatc tggccgcggg
     4561 catcgaccat ctggctggcg attgtccgct tccagatcag ctcgaacagt cggtactcgt
     4621 cgtcgggcag ttgccccttc agcacgctgg gcagctcgaa cggatggccg gcggggcgga
     4681 tcgcctcgtg ggcctcctgg gcgttcttga ccttcgattt gtagatccgc ggcgactgcg
     4741 gcagatactc gccgccatac tcggcccgca cgaggtcgcg ggccgcgtcg atcgcgacct
     4801 gggcgaggct cgttgaatcg gtccgcatat aggtgatgtg gccgttctga tacaggctct
     4861 gggcggcgtt catcgtccgc cgcgcgccga agttcagctt gcggttggct tcctgctgga
     4921 gcgtgctggt ggtgaagggc tccggcggct tgcgggtgta aggccgctgc tccacgcttt
     4981 tgacgcggaa ttggccgcgc ttcagccgct cggccaattc gtgggctttt tgctcatcga
     5041 gcagcaagtg ctgcgggtct ttgatcttgc cggtcgccgc gtcgaaatcc ttgccggagg
     5101 gaatcttgcg cccgccgaca ctgaccagct cggcatcgaa ctctttgccg ccctccgcgg
     5161 cgaggcgggc gatcaagtcc caccacgtgg ccgtgcgaaa ggcgatccgt tgtcgctcgc
     5221 gctcgacgat cagccgcacc gcgacgcttt gcacgcgtcc cgcagaaagg cccgtgcgga
     5281 ccttcttcca caagaggggc gagacttcat acccgtatag cctgtcgaga atccgccgcg
     5341 tttcctgcgc ccgcaccagg ccgtcgtcga tgtcgcgggg attcgccagg gcgtcctgaa
     5401 tcgcctcctt cgtgatctcg tgaaacacga gccggcggac gggcactttc ggcttgagga
     5461 cttcgcacag gtgccaactg atggcttccc cttcgcggtc ttcgtccgtc gcgagaaaga
     5521 gctggctact gcctttcagg agatcgcgga gcttggccac ctgcgacttc ttctctgggg
     5581 gcacgacgta gatcggcgtg aagttgtcct cgacgttcac gcccaggcgg gcccagggct
     5641 ggcccttgta ttgcgccggc acttctttgg ccccctgcgg gaggtcgcgg atgtgcccga
     5701 tgctggcctc gaccgtgtag ccccctccga ggaacttgga gatcgtcttg gccttggcgg
     5761 gcgattcgac gatgaccagc gatttgccgg tcgcggggct tttggcggca cgtttggcca
     5821 tcgggtgggt ccggaaggcg ggaatttccg atcgggacgg gcgggcaaac cttgtcgtgt
     5881 atatagcaaa ccttgtcgtc tatatatctg gagggtccgc cggcggcctg aacgtcaagt
     5941 tcgccgtctg gcaagaccct cccattcgct gccggtcgca aggcgtggtg ccggcagcct
     6001 ttgccgggct gccatgcatg aatcggccga ccggtaccag cgcgtgccac cggcagcat
//
LOCUS       DASUGB010000150         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1406728, whole genome shotgun sequence.
ACCESSION   DASUGB010000150 DASUGB010000000
VERSION     DASUGB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406728"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV87_02965"
     CDS             <1..225
                     /locus_tag="VFV87_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5102742.1"
                     /translation="GNLLVAGKTMAQTFLANSATRLHPIDWSTGTAAAHTARKGWTTR
                     QLLEHVSELQPPGGEAHANRLVDCQHSTRQ"
     gene            362..1930
                     /locus_tag="VFV87_02970"
     CDS             362..1930
                     /locus_tag="VFV87_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102743.1"
                     /translation="MKRFLKRHADQVSGVLSGFDRLRFRGTMRWLAYADGMRSFLSCA
                     GVLLKDFQSYVQGVTGRIREAAERLAAEADRPLVYLNSSAASKEELARTIAERDGVRE
                     GLVCVLSAVESCYSYEVHRSRERRLLELRSRPQKCLHYYFYLEDPTFGFMHVRLQTWF
                     PLNVHVAINGREWLGRQLARAGIDCRRADNCFLELADLQEAQRLADAQLATRWKEVFD
                     KLIAQVHPVHRQLLYAGKPIEYYWSLEQSEWATDLMFRSPQELARFYPAWLRRGLDFT
                     SPEVMRFLGKKTPKHGGVNGHFAGDVVSDVGRRADHVRIKHRLGGNWIKMYDKHGAVL
                     RVETTLNDVRGMKVFRRAEGDACGKRLWRSLRKGVADVKRRAQISEAANHRYLDALAE
                     VNAPESLAQLVDALCRPAELNGRRVRALHPWSPEDSRLLAAVNRPEFAIAGFRNRDLR
                     PLLFDARTASPEEARREGAKVTRQLRMLRAHGLILKVPHTHRYHLTASGRKALTALQA
                     ARQASAEQLAQLAA"
     gene            2183..2407
                     /locus_tag="VFV87_02975"
     CDS             2183..2407
                     /locus_tag="VFV87_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX5102744.1"
                     /translation="MRAPSLLRPAGQMQQQLADLLGISQPCLSKMESQDDMQISTLSR
                     LVESLGGRLELIAHMPKGDVRITQFGNQTA"
BASE COUNT          454 a          736 c          810 g          422 t
ORIGIN      
        1 ggcaacctgc tggtcgccgg caagacgatg gcccaaacct tcctcgccaa ctccgccacg
       61 cgactgcacc ccatcgattg gtccaccggc acagccgccg cccacacggc aagaaaaggt
      121 tggaccaccc gccagcttct agagcacgtt agtgagctgc agccccctgg cggcgaagcg
      181 cacgccaatc gactggtgga ttgccaacac tccacccgcc aataagtgaa cgcacgtgga
      241 actaaggggc gcagttgtca aagcgcagcc tccggccgca accgaaggga agtgaggcgt
      301 tgctcctagt cgtatagtgg cgggtattca gccttcgcca ctgtgttcca ggagcaacgc
      361 catgaagcgg tttctcaagc gtcacgccga ccaagtgagc ggcgtgttga gcggtttcga
      421 tcgattgcgg tttcgcggga cgatgcgttg gctggcttac gccgacggca tgcgatcgtt
      481 tctgtcctgc gcgggggtgc tgttgaagga ctttcagagt tacgtccaag gggtgactgg
      541 ccggattcgc gaggccgccg agagactcgc cgcggaagcg gatcggccgc tggtctatct
      601 gaacagctcg gccgccagca aggaggagct ggcccgcacg atcgccgagc gggacggcgt
      661 gcgcgagggt ctcgtctgcg tcttgagcgc ggtggagtcc tgctactcct acgaggtcca
      721 tcgcagccgc gagcgacggc tgttggaatt gcgcagccgc ccccagaagt gcctgcacta
      781 ttacttctat cttgaggacc ctaccttcgg cttcatgcac gttcggctgc agacctggtt
      841 tccgctgaac gtccatgtgg caatcaacgg ccgcgagtgg ctgggacggc aactggcgcg
      901 cgcgggaatc gactgccggc gcgcggacaa ctgctttctg gaactggccg acctccaaga
      961 agcgcagcgg ttggccgacg cgcagctggc aacccgctgg aaggaggtct tcgacaagct
     1021 catcgcgcag gtccatccgg tccatcgcca gctgctctac gcgggaaagc cgatcgagta
     1081 ctattggtcg ctggagcaga gcgaatgggc cacggacttg atgttccgct cgccgcagga
     1141 gttggcccgg ttctatcccg cctggctgcg gcggggactg gacttcacca gccccgaggt
     1201 gatgcggttt ttgggcaaga agacgcccaa gcacggcggg gtcaacggcc atttcgccgg
     1261 agacgtcgtc agcgacgtcg gccgccgcgc cgatcatgtg cggatcaagc accgcctcgg
     1321 cggcaactgg atcaagatgt acgacaagca cggcgcggtc ctgcgggtgg aaaccaccct
     1381 caacgacgtg cgcggcatga aggtcttccg ccgcgccgag ggggacgcct gcgggaagcg
     1441 cctctggcgc tcccttcgca agggcgtggc cgacgtgaag cggcgggcgc agatctcgga
     1501 ggccgccaac catcggtatc tggacgccct ggccgaagtg aatgctcctg aatcgttggc
     1561 ccaactggtc gacgcgctct gccggccggc ggagctgaac ggtcgccgcg ttcgagccct
     1621 gcacccctgg tcgccggagg actcccggct gctggcggcg gtgaatcgtc cggaattcgc
     1681 catcgccggc tttcgcaacc gcgacctgcg gccgctgctg ttcgacgcca ggacggcttc
     1741 gcccgaagag gctcgccggg aaggagccaa agtcacccgt caattgcgga tgctgcgagc
     1801 tcacggcttg attctgaaag ttccccacac ccatcgctat catctcaccg ccagcggccg
     1861 gaaggccctc accgccctcc aggccgctcg acaagccagc gccgagcagc tcgcccaact
     1921 cgccgcgtga aatcttcgcg ccgcacgaag gattgaatgg ttattaataa agagcgcatg
     1981 gcatttgagc cggaggtgag tataactgat cttgcgtaca gtatcaacgg aagtttttgg
     2041 gccacttgga aatgagtgag gtaacaggag gtcgcgaagg ccgcaaagag gtgtagggtg
     2101 gggtgctttg cgcccacgcg gtacggggac caggctaccg gacgcgatgc ggtgagtgcg
     2161 tttcgagtgg agcgaaaggt gggtgcgagc acccagccta ctgaggcccg cggggcagat
     2221 gcagcagcag ttggccgatc tgctgggcat ttcgcagccg tgcctgtcga agatggaaag
     2281 ccaggacgac atgcagatca gcacgctgag ccggctggtg gagtcgctgg gcgggcggtt
     2341 ggaattgatt gcgcacatgc cgaaggggga tgtgcggatt acgcagtttg ggaaccagac
     2401 ggcttgaggc gacgttgttt ag
//
LOCUS       DASUGB010000151         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_156639, whole genome shotgun sequence.
ACCESSION   DASUGB010000151 DASUGB010000000
VERSION     DASUGB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156639"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFV87_02980"
     CDS             <1..730
                     /locus_tag="VFV87_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5102745.1"
                     /translation="VKLQLQEVTAHQYQIGYGEGAGAGFAGGTSKGKVRLIRLEYDGG
                     DWDLNHGPGGDENMLFEYGVLTQQKVSEKSESRRVSQLANFPPDKSPPVVFLTGQKSL
                     SLSNNDIKVLREYLIDKHGMLFASSGSAHFHNQFVATMNRVLPDVRPVPIPLDDTIHR
                     VPFAVPFLPYVVPHGGKDALGWSMDGRWLVYYHPGDISDAWADGHAGVSPEIYNSCYQ
                     LGANVINYGHAEYAKWLMAKGKPK"
     gene            823..2385
                     /locus_tag="VFV87_02985"
     CDS             823..2385
                     /locus_tag="VFV87_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5102746.1"
                     /translation="MSVRADELNELSLEGWKQLREVERYQLQIAEKYWREKNYKVAAA
                     EYEKFMELYESSTGAPYAQLKWSLCEVELRQQNTAIKEGFQSVIDYWPDSPQAIAAKY
                     YIGQTQKEIGKLKEAKATLKDVVARYPQNLAAVFAIRDLVDITTIENDIPARVELWKK
                     LVFDFKRTRDSNGACVQASQQLAAHWFREVAFDDAVKSLATTYSPEQLPMQVNAYVRS
                     PISDLTGAAETKAKGDKLADQAIAWMQRTVPADQSTPEAKQLARQVLFCVADLYTVAR
                     RAEQVPAAYEAILKNASPDDEALGRLAQWWKTQSNYDKARETYRRFADAPAGLAQVAH
                     SFREQQKYDEAIQTYQQVAAQDSERRVQWKSELALTYRYAKKWPEAVAVYEELFKEDI
                     ENANRWRWEVAMTFRDSGNYPQAIGNFRQCTNFPENYQQMAWCHRQAKQPSEAITLYQ
                     QIAGGSQPHAPWAMLQIAYTREEAGEKEPAIAAFQQVCKKYPKDQHASVAHAHLQQQY
                     KISVTLGGAAEE"
     gene            complement(2404..2476)
                     /locus_tag="VFV87_02990"
     tRNA            complement(2404..2476)
                     /locus_tag="VFV87_02990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2441..2443),aa:Gly,seq:ccc)
     gene            2680..3726
                     /locus_tag="VFV87_02995"
     CDS             2680..3726
                     /locus_tag="VFV87_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX5102747.1"
                     /translation="MLNRSLLVVVAIACQSTIGVGLSTGQEKAEEAPDKLVLPEFPRV
                     NLAPWYVVDPNWPEKPAEYEWAAMPGMAVDAQDNIWTFNRGQPPVQVYRPNGKLVRAW
                     GDDTIGSAHHLKIDRNGNIWIADIGLHVVRKFSPGGEILLTIGTPGKRGADQTHLHAP
                     TDMAISPAGDVFVSDGYGNNRIVHFDAKGKYVKEWGQMGVGPTDFSLPHAIAMDSQGK
                     LYVADRNNVRVLVYDQAGKLLDTWADVICPWGFCMTAKDELWVCGSSPMPWREDPDYP
                     GAPVSCPPKDQVVMKFNTLGRLLQLWTVPKGEDGKEQPGDLNWAHCVAVDSQGNLYLG
                     DIIGKRAQKFVLQK"
     gene            complement(3736..>4273)
                     /locus_tag="VFV87_03000"
     CDS             complement(3736..>4273)
                     /locus_tag="VFV87_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102748.1"
                     /translation="AGVLYLVAGLMQLGLFSAAAPLVHSIAASTITLLAHLGIASAVM
                     LYARHVVLDAQGRLKVHIDPDRRKGKKPKSRAKLKVVKADKSDDDSTVSAPASTKTAP
                     AASEPAKPAAQPRFGWGGGGAANAPAKASAAVSKSSAPYDADDDEEDENEMDGQKLSR
                     AERKRLKKLARRDQRRAA"
BASE COUNT          861 a         1282 c         1403 g          727 t
ORIGIN      
        1 ggtgaagctg cagcttcagg aagtcaccgc ccatcagtac cagatcggct atggcgaagg
       61 agccggcgcg ggcttcgccg gcggcacgag caagggcaag gtgcggctga tccggctgga
      121 atatgacggc ggcgattggg acctgaacca cggccccggc ggtgatgaga acatgctgtt
      181 cgaatacggc gtgctgacgc agcagaaagt gtcggagaag agcgagtcgc ggcgcgtctc
      241 acagcttgcc aattttccgc cggacaagag cccgccggtc gtgttcctga ccgggcaaaa
      301 gagcttgtcg ttatcgaata acgatatcaa ggtgctgcgc gagtacttga tcgacaagca
      361 cggcatgcta ttcgccagca gcggcagcgc ccactttcac aaccagttcg tcgccacgat
      421 gaaccgcgtc ctgcccgacg tgcggcctgt gccgatcccg ctcgacgaca cgatccaccg
      481 cgtgccgttc gccgtgccgt ttttgccgta cgttgtgccg cacggcggca aggacgccct
      541 cggctggtcg atggacgggc ggtggctcgt ctattaccac ccgggcgaca tcagcgacgc
      601 ctgggccgac gggcacgcgg gcgtgtcgcc ggagatctac aactcgtgtt accaactggg
      661 cgcaaacgtc attaactatg ggcacgcgga atacgcgaag tggctgatgg ccaaaggaaa
      721 gccgaaatga ggtcgaggca gattgaaaaa tgcgaattgc aaaatgcaaa ttgcaaattg
      781 attttcacgc tcggtattgc cgcctgtttg acggtattcg ctgtgtccgt ccgcgctgac
      841 gagctgaacg aactctccct cgagggctgg aagcagcttc gcgaggtcga gcgctatcag
      901 ctccagatcg ccgagaaata ctggcgcgag aagaactaca aggtggcggc ggcagaatac
      961 gaaaaattca tggagctgta cgaatcgagc acaggagcgc cctatgccca gctcaaatgg
     1021 agcctgtgcg aggtcgagct ccgccagcag aacacggcca tcaaggaggg ttttcagtcg
     1081 gtgatcgact actggcccga ttcgccgcag gcgattgccg cgaagtacta catcggccag
     1141 acgcagaagg agatcggcaa gctgaaggag gcgaaggcga cgctgaagga cgtcgtggcg
     1201 aggtacccgc agaatctggc ggcggtgttt gcgattcgcg atctcgtgga catcacgacc
     1261 atcgagaacg acatcccggc ccgcgtcgag ctgtggaaga agctggtctt cgatttcaag
     1321 cggacccgcg attccaacgg ggcctgcgtg caggcctcgc agcagctcgc ggctcattgg
     1381 ttccgcgaag tcgcgttcga cgacgcggtc aagtcgctgg ccacgaccta ttcgcccgag
     1441 caactgccga tgcaggttaa tgcctacgtc cgctcgccca tttcggacct gaccggcgcg
     1501 gccgagacga aagctaaggg ggacaagcta gccgatcagg caatcgcctg gatgcagcgg
     1561 accgtgccgg ccgatcaatc gacgcccgag gccaagcaac tcgcccggca ggtgctgttc
     1621 tgcgtggcgg acctgtacac ggtcgctcgc cgcgccgaac aggttccggc cgcttatgag
     1681 gcgatcctga agaacgccag ccccgacgac gaggcactcg gccgcctggc gcagtggtgg
     1741 aagacgcaga gcaactacga caaggcccgc gagacgtatc gccgctttgc ggacgcgcca
     1801 gccggcctgg ctcaggtcgc ccacagcttt cgcgagcaac agaagtacga cgaggcgatt
     1861 caaacctacc agcaggtcgc cgcacaggac agcgagcggc gcgtgcagtg gaagagcgag
     1921 ctggcgttga cgtatcgata tgcgaaaaag tggcccgagg cggtcgccgt ttatgaggag
     1981 ctgttcaagg aggacatcga gaacgccaat cgctggcggt gggaagtggc gatgacgttt
     2041 cgcgattcgg gcaactatcc gcaggcaatc ggcaacttcc gccaatgcac gaactttccg
     2101 gagaactatc agcagatggc ctggtgccac cggcaggcga agcagcccag cgaggcgatc
     2161 accctctatc agcagatcgc gggtggttcg cagccacatg ctccctgggc catgctgcag
     2221 atcgcttaca cccgcgagga agcgggcgag aaggagccgg cgatcgccgc cttccagcag
     2281 gtctgcaaga agtatcccaa ggaccagcac gccagcgtgg cccacgccca cctgcagcag
     2341 cagtacaaga tcagcgtcac gctaggcggg gcggcggaag aataagggcc ggagaatgcc
     2401 aagagcgggc gacgggactc gaacccgcga catccagctt gggaagctag tgctctacca
     2461 actgagctac acccgcaaat cgaccggccc aagcggctac accggcaagt atagaggacg
     2521 gggcggcgtt tgcaagaatg acggtttgtg gccggagggg gttcgcggga ttcgctgcgc
     2581 gcagtccggg tagcgctggg atgaatcgac acgctgggcg cggtagagtg atgccggttt
     2641 cgtccagtcc tctcccatct ttcgcccgcg agtcgcaaaa tgctcaaccg ctcgttgctt
     2701 gttgttgtcg ccattgcgtg tcagtcgacg atcggtgtcg gcctctcaac cggccaggag
     2761 aaggcggagg aggctcccga taagctcgtg ttgcccgaat ttccgcgggt caacctggcg
     2821 ccgtggtacg tggtcgatcc caactggcca gagaagccgg cggagtatga gtgggcggcg
     2881 atgccaggca tggcggtcga tgcccaggac aacatctgga cgttcaaccg cggccagccg
     2941 ccggtgcagg tctatcggcc caacggcaag ctcgtacggg cctggggcga cgacacgatc
     3001 ggcagcgctc accatctcaa gatcgatcga aacgggaata tctggatcgc cgacattgga
     3061 ttgcacgtcg ttcgcaagtt cagccccggc ggcgaaatcc tgctgacgat cggcacgccg
     3121 ggcaagcgcg gtgcggatca gacccacctg catgctccca ccgacatggc catcagcccg
     3181 gccggcgatg tgttcgtctc cgacggctac ggcaacaacc gcatcgtcca cttcgatgcc
     3241 aagggcaagt acgtgaaaga gtggggccag atgggtgtcg gaccgaccga tttcagcctg
     3301 ccgcacgcca ttgcgatgga tagccagggc aagctgtacg tcgccgatcg caacaacgtc
     3361 cgcgtgctgg tctatgacca ggcgggcaag ctgctcgata cctgggcaga cgtcatttgt
     3421 ccgtggggct tttgcatgac ggccaaggac gagctgtggg tctgcggctc gtcgccgatg
     3481 ccctggcgcg aagatcccga ctatcccggc gcgcccgtga gctgcccgcc caaggatcag
     3541 gtggtgatga aattcaacac cctcggtcgg ctgctgcagc tttggacggt tcccaagggc
     3601 gaagacggca aggagcagcc gggcgatctg aactgggccc actgcgtggc ggtcgattcg
     3661 caggggaatc tgtatctggg cgacatcatc ggcaagcggg cgcagaagtt cgtgctgcag
     3721 aagtgaccgc cagcgctacg ccgcccggcg ctgatcgcgg cgggcgagct ttttcaggcg
     3781 tttgcgctcg gcgcgcgaga gcttctggcc gtccatctcg ttttcgtcct cttcgtcgtc
     3841 gtctgcgtca taaggcgccg aggatttcga gaccgcggcg ctggccttgg ccggagcgtt
     3901 cgcagcaccg cctccgcccc agccgaaacg cggctgcgcg gccggtttcg cgggctcgct
     3961 ggcggccggc gcggtcttgg tagaagcggg agccgagacg gttgaatcgt cgtcggactt
     4021 gtctgccttt accactttga gcttcgcccg gctcttgggc tttttgccct tcctgcgatc
     4081 gggatcgata tgcaccttga gccggccttg ggcgtcgagc accacgtgcc gggcatagag
     4141 catcactgcc gacgcgatcc ccaggtgtgc aagcaaggtg atcgtagatg cggcaatgga
     4201 atggaccagc ggagcggcgg ctgagaacag gccgagttgc atcagaccgg cgaccagata
     4261 gagcacgccc gcg
//
LOCUS       DASUGB010000152         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_990831, whole genome shotgun sequence.
ACCESSION   DASUGB010000152 DASUGB010000000
VERSION     DASUGB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990831"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..2341
                     /locus_tag="VFV87_03005"
     CDS             149..2341
                     /locus_tag="VFV87_03005"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5102749.1"
                     /translation="MSTTDYVKLTRQVIRTLLTLLLLTGLLCAQSSFKLTGVIEQLPT
                     SGLTGTWKIAGKLLAIRQSTEIDQEKAKAALGALAVVESEGTYQGLPLAKKVTILQAA
                     SSSPNTPSPTGYRIVAWNDLGMHCMDATFDVFAILPPFNDLKAQLINNQGKLVTSPNG
                     ITLTYQAVADPDGSINRTSVNKTNFWAQIVNLFGPSANLPADTGLIGNRMPGASNTPQ
                     PMPFQQGFHWFAADGIPITPYDDAGRKNYYPMMLVTARDSAGQLLAQSKVVLPVSDEM
                     DCRLCHGSNTSTAARPSKGWINLAGDPDREYRYNILRLHDDRQPPASIAGLLASAKLN
                     PAGLEATALSGKGVLCAACHSSNALPGSGLAGVPPFTQSMHGGHSSVTDPLNGLLLNQ
                     SSNRAACYRCHPGSETRCLRGVMGNSVAPDGSLAIQCQDCHGTMSAVGAPGRIGWLDQ
                     PSCQNCHTGTATSNSGQIRFTSAFDAAGNRRAAANLTFATKPNTPAPGFNLYRFSTGH
                     GGLQCEGCHGSTHAEYASSHRNDNIQSIETQGHIGTISECSACHTTVPSTANGGPHGM
                     HSVGSSWVSGHGSFVERSGSGACRSCHGGDLRGSVLSRSLADRSFSTDFGRKTFWRGF
                     TIGCYACHNGPNSESASRNRAPLVTNASVSTTAGKSVTLPLQATDSDGNPLTLRVVSQ
                     PAHGTVGLSGFNATYFPEPNFTGSDSFTFAAWDGSIDSNLGSINVTVR"
     gene            2500..3855
                     /locus_tag="VFV87_03010"
     CDS             2500..3855
                     /locus_tag="VFV87_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall biosynthesis glycosyltransferase"
                     /protein_id="HEX5102750.1"
                     /translation="MEIAQKADPASSLSADACAAVADLNQADIVIGIPSYNNVRTIGH
                     VVRAAQAGLQKYFPQYRSVIVNSDGGSKDGTPDAVLGVEAADPHLLLLNTPLTAVHRL
                     SFPYHGVPGKGSAFRMIFQIAANLNARACCVVDSDLRSITPEWIDLLVRPILHDGYDF
                     VAPYYHRHKYDGTITNSIVYPLTRALYGRRIRQPIGGDFGLSNPLVRRYLQHEDWESD
                     VARFGIDIWMTTTAVAEDFKVCQAFLGAKLHDAKDPGADLSAMLQQVLSSLFSLTDRY
                     HSAWQAREGSVPSELFGFRYDVGLEPIAVNVDRMLAAFRSGFEDLRDVWALALSPNTM
                     RALAGLARATGESDAAESFHIDDRLWAGIVYEVAAAWKRLAVERGHILRSFTPLYMAR
                     VASWVIETRALDAPAVEERMEGLCLTFESMKPYFIELWEHPDTPHPDDVLVAAKDSHH
                     V"
     gene            3848..>4344
                     /locus_tag="VFV87_03015"
     CDS             3848..>4344
                     /locus_tag="VFV87_03015"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102751.1"
                     /translation="MFETINEIFNRTWSQLYAGLTFYIPPLFVAFVVIAVAWLAAHFV
                     RFLVSHLVKGLAFDRFLRSTGLSLLVRPQGELRTGPLAAKAVFWIVFLGGMLAAVSVF
                     ETQWTRRIVEAVVLLLPNVLTAAAILIAGLWLSLYLSRSVLLWAAEERMPTPRRWASA
                     VRVVV"
BASE COUNT          813 a         1578 c         1082 g          871 t
ORIGIN      
        1 gcgggctgaa ctcggaaccc aatcttctcc gcaaagctgt cgtaacggaa cctaaggtgc
       61 cgggtggggg gacttctgtc ttcatcgcgc ctcatcgcct ctttcggccg ctcttcaagg
      121 atttcggatt tcaaggaagg actagtccat gtctaccacc gactatgtaa agcttactcg
      181 ccaagtaatc cgtacactgt tgactttgct gctacttact ggacttttat gtgcacaaag
      241 ctcattcaag ctcactggtg tcattgagca actccccaca tcgggcctga ccggcacctg
      301 gaagatcgcc ggcaaattgc tcgcgattcg gcagtcaaca gagatcgacc aggaaaaggc
      361 caaagccgcc ctcggtgccc ttgcggttgt ggaaagcgaa ggaacttacc aagggcttcc
      421 tctcgccaag aaggtcacca tcctgcaagc tgcctcttct tccccaaaca cgccgtcgcc
      481 caccggttac cgcatcgttg cttggaatga cctcggcatg cactgcatgg acgcaacgtt
      541 cgacgtcttt gccatactcc cgcccttcaa cgacctgaag gctcaactca tcaacaacca
      601 aggcaaactc gtcacctcgc ccaatggaat cacgctcacc taccaggccg tcgctgatcc
      661 ggacggttcc atcaaccgca cctcggtgaa caagacgaat ttctgggcgc agatcgtcaa
      721 cctgttcggc ccctcggcca atttgcccgc cgataccggt ctcattggca acaggatgcc
      781 cggcgcttcc aacactcccc aaccgatgcc gttccagcaa ggcttccact ggttcgccgc
      841 tgacggcatc cccatcacgc cctacgatga cgcaggccgc aaaaactact accccatgat
      901 gctcgttact gcacgcgaca gtgccggtca gcttctggca cagtcaaagg ttgttctgcc
      961 cgtctccgac gagatggatt gccggctctg ccacggctcc aacacctcca ctgccgcacg
     1021 accctccaaa ggctggatca accttgccgg cgatcccgat cgcgagtacc gctacaacat
     1081 cctccgcctt cacgatgacc gccagccgcc ggcctccatc gctggcctgc tggcaagcgc
     1141 caagctcaat cccgctggcc tcgaagccac ggcgctctcc ggcaaaggcg tcctttgcgc
     1201 tgcctgccat tcctccaacg cgctgcccgg ttcgggactc gccggcgttc cgcccttcac
     1261 gcaatccatg cacggtggac attcgtccgt aacggatcca ctgaatggcc tgctcctgaa
     1321 ccagtcctcc aaccgcgccg catgttatcg ctgccatccc ggatcggaga cacgatgcct
     1381 ccgcggcgtg atgggcaatt ccgtcgcgcc cgacggttca ctcgccattc aatgccagga
     1441 ctgccacgga acgatgagcg ctgtcggcgc ccctgggcgc atcggctggc tcgatcagcc
     1501 ctcctgtcag aactgccaca ccggaactgc aaccagtaac tccggccaga tccgcttcac
     1561 ctcagccttc gatgctgcgg gcaaccgtcg cgctgccgcc aatctcacct tcgccacaaa
     1621 gcccaacacg cccgctccgg gatttaacct ttaccggttc tccactggcc acggcgggct
     1681 gcaatgcgaa ggctgccatg gctccacaca cgccgagtac gcctcctcgc accgcaacga
     1741 caatattcaa agcatcgaaa cgcagggcca catcggaacg atcagtgaat gctcagcctg
     1801 ccacacaact gttccaagca ccgcgaacgg cggtccgcac gggatgcact cggtaggttc
     1861 gtcttgggtc tcgggccatg gcagtttcgt tgaacgctca ggctcgggcg cttgccgctc
     1921 ctgccacggc ggcgatctgc gcggctccgt cctctcacgg tcgctggccg accgcagctt
     1981 cagcaccgac tttggcagaa agaccttttg gcgcggcttc accatcggat gttatgcctg
     2041 ccacaacggc cccaattcgg aaagcgccag ccgcaatcgc gcccccttgg tgaccaatgc
     2101 ctcggtttcc actaccgccg gaaagagtgt cacgctcccg ctgcaagcca cagactccga
     2161 cggcaacccg ctcaccctgc gggtggtgtc gcagccggcc cacggcaccg tcggcctctc
     2221 gggcttcaac gccacctact tccccgagcc aaacttcacc ggtagtgaca gcttcacctt
     2281 tgccgcttgg gacggctcta tcgattcgaa tctcggttca atcaacgtca ccgttcgcta
     2341 aacatcggcg ccgtcgacaa tgacggcggt aggaccgacc agcgtaactt caaagcaact
     2401 tgccgtgagc gcgtgcgcgc cggccaccaa ctgccggtgc acccgtacct gtgcgatgca
     2461 cccgatgcaa cgcacggcct attgtattaa gcttttgtca tggagatcgc gcaaaaagcc
     2521 gatcccgcct cgtcgctctc cgccgatgct tgcgctgctg ttgccgattt gaaccaggcc
     2581 gacatcgtca ttggcattcc cagctacaac aacgtccgca ccatcggcca tgtcgtccgt
     2641 gcggcgcaag ccgggttgca gaaatacttt ccccaatacc gctccgtcat cgtcaactcc
     2701 gatggaggtt ccaaagacgg aacgcccgac gctgtcctcg gcgtggaagc cgccgacccc
     2761 cacctcctcc tgctcaacac acccctcacc gcagtccacc gcctctcgtt tccctaccat
     2821 ggagtccccg gcaaaggctc cgcgttccgc atgatctttc aaatcgccgc aaatctcaac
     2881 gctcgtgcct gttgcgtggt cgactccgac ttgcggtcca tcacccccga gtggatcgat
     2941 cttctcgtcc ggcccatcct ccacgacggc tacgatttcg ttgcccctta ctaccaccgc
     3001 cacaaatacg acggcactat caccaactcc atcgtctatc ccctcacgcg cgccctctat
     3061 ggacgccgca tccgtcaacc catcggcggc gacttcggtc tctcaaaccc gctcgtgcgc
     3121 cgctacctcc agcacgagga ttgggagtcc gacgttgccc gcttcggcat cgacatctgg
     3181 atgaccacca ctgccgtggc cgaagacttc aaagtctgcc aagccttcct cggcgccaag
     3241 ctgcacgacg ccaaggatcc cggcgccgac ctctccgcca tgctccagca ggttctctcc
     3301 agtctcttct ccctcaccga tcgctaccac tcggcatggc aggcccgcga aggcagcgtt
     3361 cccagcgaac tgttcggttt ccgatacgac gtcggcctcg aacccatcgc cgtaaacgtc
     3421 gaccgaatgc tcgccgcgtt tcgcagcggc tttgaagacc tgcgcgatgt gtgggccctg
     3481 gctctctcgc cgaacaccat gcgtgcgctc gccggcctcg cccgcgcgac tggagaatcc
     3541 gacgccgccg aatcgttcca catcgacgac cgtctttggg caggcatcgt ctacgaagtg
     3601 gctgcggcat ggaagcggct cgccgtggag cgcggccaca tcctccgctc gttcacccca
     3661 ctctatatgg cgcgcgtcgc cagttgggtc atcgagactc gcgctctcga cgcccccgct
     3721 gtggaagagc gaatggaggg gctatgcctc acctttgaat caatgaaacc ctacttcatc
     3781 gaactgtggg aacatccaga caccccgcat cccgatgacg tcctcgtagc cgcgaaggat
     3841 agccaccatg tttgaaacga tcaacgaaat cttcaaccgc acctggagtc aactctacgc
     3901 cggtctcact ttctacatcc cccctctgtt tgtcgccttc gtcgtcatcg ccgtcgcctg
     3961 gctcgccgct cactttgtcc gcttccttgt atcccacctg gtaaagggcc tcgccttcga
     4021 ccgcttcctc cgatccaccg gactctccct cctcgttcgc ccgcaaggcg aactgcgaac
     4081 cggcccgctc gccgcaaaag ctgtcttctg gatcgtcttc cttggcggga tgctggccgc
     4141 cgtcagtgtc tttgaaacgc agtggaccag gcgaatcgtc gaagccgttg tcctcttgct
     4201 tcccaacgtt cttaccgcgg ccgcaatttt gattgccggc ctctggctga gtctctatct
     4261 gtcccgcagc gttctcctgt gggccgccga ggagcggatg cccacaccgc ggcgttgggc
     4321 ctcggccgtt cgcgtcgtcg tcat
//
LOCUS       DASUGB010000153         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1963613, whole genome shotgun sequence.
ACCESSION   DASUGB010000153 DASUGB010000000
VERSION     DASUGB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963613"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..2504
                     /locus_tag="VFV87_03020"
     CDS             165..2504
                     /locus_tag="VFV87_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5102752.1"
                     /translation="MKRVCLAVVVALAAAPALRADPPHASYIFPAGGQRGTTVDVKVG
                     GHFLHERAAFEMLGPGVTAPGELVRGETVWFEGPLIRQPASQRSEDYPQDYAGQIAIA
                     ADSPPGERWWRCWNAQGVTAALPFAIGDLPEVVELEIDGDPIPVPVTLPVTINGRVFP
                     REDVDIWTFEAQAGQSITCSVIAKQLGSPLTAKLEVRDPSGKSVAESTGTALDEARLR
                     FATPVSGRYAVHITDAEAGGLQHYVYRLTVTAGPWIDHVYPLGGKRGSQVRLETIGQA
                     IPDSAVSLQIPDDAPRIIQPRIEVAAQAVTLPKFEISDQPEHLEAEPNESAGQAAQIA
                     VPAIINGRIQKAGDVDVWWVELAAGKPVQFDARTSQLGSPLALVLTVRDPAGKQLFQA
                     DATASKDDLSATFTPAADGVHLIEIVERFARRGGPEFAYRVHVGFPQPDFQLFVPDSL
                     SVDVGGQKNVEVQIERRGEMKSPITLHLDDLPAGVTCEDVVCQPQTGKATLTLKAAAD
                     APVISSHVRLIGKADVNGQTVERVSKIAGQKDGFSRGIEIPSRLTTTLPTPFKYVGQY
                     SFQFAPRGAVLRKRFTIDRGGFTGPIEVCLADRQGRHLQGVTGPVLTIPPEASEFEYP
                     LALPPWMELGRTTRSNLMLTGEVQDAAGKSYQVCYSTNEQNQQMIGLVTAAPLRLALE
                     RNSYSVAPGGELAIPVAIKRDPSITSPIQLELVVPRHMQDISAQPVTAAAGAEQETVT
                     LRLGEHPGPLNMPLLIRATGERNGDPVIAEAEIELVPAR"
BASE COUNT          436 a          849 c          834 g          424 t
ORIGIN      
        1 taagcgagag cgtcattccg gctcgggccg cttagttgct taacctcctt cacccgccgg
       61 acgagcgcgc aggggtcgca tggcaaatgc cgcgtaatcg gcttccgctg gcccgcggga
      121 gaacgcataa tcgttgggtg atgtgtttgc gaggaggaat cggcatgaag cgagtttgcc
      181 tggccgtcgt cgttgccctt gccgccgcgc cggcgctccg cgccgatccg ccgcatgctt
      241 cgtatatctt cccggcgggc ggccagcggg gaacgacggt cgatgtcaag gttggcgggc
      301 attttctgca cgagcgggcc gcgtttgaga tgctcgggcc gggcgtgact gcgccgggcg
      361 agctggtgcg cggggagacg gtctggttcg agggtccgct gattcgccag ccggcgagtc
      421 agcggtcgga agattatccg caggactacg ccggccagat cgccattgcc gccgatagcc
      481 cgcctggcga gcgctggtgg cgatgctgga acgcccaggg ggtgacggcg gcgctgccgt
      541 tcgcgatcgg cgatctgccg gaagttgtcg agttggagat cgatggcgat ccaatccccg
      601 tgccggtcac gctgccggta acgatcaacg gccgcgtgtt tccgcgcgag gatgtcgata
      661 tctggacgtt cgaggctcag gcgggccagt cgattacttg ctccgttatc gcgaagcagc
      721 ttggctcgcc gctgacggcg aaactcgaag tgcgcgatcc gagcggcaag tccgtcgccg
      781 aaagcaccgg cacggcgctc gacgaagccc ggctgcggtt cgccaccccc gtttccgggc
      841 gctatgccgt ccacatcacc gacgccgaag ccggcggctt gcagcactat gtctatcggc
      901 tgacggtgac ggcggggccg tggatcgatc acgtctatcc actcggcggc aagcgcggca
      961 gccaggtgcg gctggagacg atcggccagg cgattccgga tagtgccgtt tcgctgcaaa
     1021 tccctgacga tgccccccga atcatccagc cgcgaatcga agtcgccgcc caggccgtga
     1081 cgctgccgaa gttcgaaatc agcgaccagc ccgagcacct ggaagccgaa cccaacgaat
     1141 cggccggtca ggcggcacaa atcgcggtgc cggcaatcat caacggccgg attcagaagg
     1201 cgggcgatgt ggacgtctgg tgggtggaac tggctgccgg caagccagtc caatttgacg
     1261 cccgcacgag ccaactcggc tcgccgctcg cactcgtgct gaccgtccgc gatccagccg
     1321 gcaagcagct ctttcaggcc gatgcgaccg cgtcgaaaga cgatctttcg gcaacgttca
     1381 cgccggccgc ggatggcgtt cacctgatcg aaatcgtcga gcgattcgct cgccgcggcg
     1441 ggccggaatt cgcctaccgc gtccacgtcg gcttccccca gccggacttt cagctcttcg
     1501 tccccgactc gctgtcggtc gatgtcggtg gccagaagaa cgtcgaagtg cagatcgagc
     1561 gtcgcggcga gatgaagtcg ccgatcacgc tccatctcga cgacctgccc gctggcgtca
     1621 cctgcgaaga cgtcgtctgc cagccgcaaa cgggcaaggc cacgctcacg ctcaaagccg
     1681 cggcggatgc gccggtcatc tcctcacacg tgcggctgat cggcaaagcg gacgtgaatg
     1741 gccagaccgt cgagcgggtc tcaaaaatcg ccggccagaa ggacggcttc agccgcggga
     1801 tcgaaatccc ctcgcggctg acgacgacgc ttcccacgcc gttcaagtac gtcgggcaat
     1861 actccttcca gttcgccccg cgcggggcag tgctgcgcaa gcgattcacg atcgaccgcg
     1921 gcggattcac ggggccgatc gaggtctgcc tggccgaccg gcaaggacgc cacctgcaag
     1981 gcgtcaccgg cccggtgctg acgatcccac ctgaagcgag cgagttcgag tatccgctcg
     2041 cgctgcctcc gtggatggag ctcggccgca ccacgcgctc gaatctgatg ctcacgggcg
     2101 aagtgcagga tgccgcgggc aaatcgtatc aggtctgtta ctccacgaac gaacagaacc
     2161 agcagatgat cggcctggtc accgccgcgc cgctgcggct ggctctggaa cgcaattcgt
     2221 actcggtcgc gccgggcggc gagctggcca tccccgtcgc catcaagcgc gatccgtcga
     2281 tcacctcgcc catccagttg gagctcgtcg tcccgcgaca catgcaggac atttcggccc
     2341 agccggtcac cgccgctgct ggtgccgagc aggagaccgt cacactccgc ttgggcgagc
     2401 atcccggtcc gctcaacatg ccgctgctca ttcgcgccac gggcgagcga aatggcgatc
     2461 cggtaatcgc cgaagcggag atcgagctcg tgccggcccg ctaacgccgg tggtgggcct
     2521 gcggcctgac ggccttgtcc cac
//
LOCUS       DASUGB010000154         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1685429, whole genome shotgun sequence.
ACCESSION   DASUGB010000154 DASUGB010000000
VERSION     DASUGB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685429"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFV87_03025"
     CDS             complement(<1..382)
                     /locus_tag="VFV87_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5102753.1"
                     /translation="MAFRGKESGESRVGVFDMFRGALKKTSDLLNTDVRDLFKRQGRL
                     VDDEFLGELYAIFVKTDMGTGPATRIRDRIGTDFRARVVHLEDVLKVVKDELRQRVAQ
                     PEQPIHLAASGPTVVLVVGVNGSGK"
     gene            complement(404..811)
                     /gene="nusB"
                     /locus_tag="VFV87_03030"
     CDS             complement(404..811)
                     /gene="nusB"
                     /locus_tag="VFV87_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5102754.1"
                     /translation="MSRRSRAREVVLQVLYEDDLNPDRSPEAADRFLCNRLSGDEELI
                     DFGRSLIAGVRKHRAEIDRLLVERADNWTMERMAVTDRNILRLGAFEMLHFGTPERVA
                     INEAVELAKRYGAKQSPHFVNGVLDRFMHKERR"
     gene            complement(981..1631)
                     /locus_tag="VFV87_03035"
     CDS             complement(981..1631)
                     /locus_tag="VFV87_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102755.1"
                     /translation="MMHSILLWATLSLSASPLDDGTLLFLENCNSVVEFSTKGKIGHV
                     ALVFSDPDACWVYEATPGEVRRVTLDVYYAELARINRRRDADDQIRVWAFRPTPAYSS
                     NEVGAMRTALDEQLGRRYSLRNYVRRKPGDGIHCAELTSLVLNASGRFAIENGAKVHP
                     SALYSAIELSEHYGPAGEITLAEPAKESWCLRAQRRTAEWFTWCGWSCQEAWLWCW"
     gene            1913..>2052
                     /locus_tag="VFV87_03040"
     CDS             1913..>2052
                     /locus_tag="VFV87_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5102756.1"
                     /translation="MKSALAVAAHPDDIEFLMSGTLMLLRKAGYDIHYWNLANGCCGS
                     SQ"
BASE COUNT          370 a          681 c          620 g          381 t
ORIGIN      
        1 tcttgcccga gccattgacg ccgacgacga gaaccacggt tggaccgctg gcggcaagat
       61 ggatcggctg ctcgggctgg gcgacgcgct ggcgcagctc gtccttgaca accttcagca
      121 catcctccag gtggacgacg cgagcccgga aatcggtccc gatgcggtcg cggattcgcg
      181 tggccggtcc cgtccccatg tcagtcttca cgaagattgc atacagctct ccgagaaact
      241 cgtcatccac caagcggccc tgccgcttga agagatcgcg gacatcggta ttgagcagat
      301 cgctggtctt cttcagcgct ccgcggaaca tgtcgaacac gcccacgcga ctttctcctg
      361 actctttgcc ccggaacgcc acggagggcg ttccctacag cgcctatcgc cgctctttgt
      421 gcatgaaccg atccagcacg ccgttgacga agtgcggcga ttgcttcgcg ccatagcgct
      481 tggccagctc gaccgcctcg ttgatcgcca cgcgctccgg cgtgccgaag tgcagcatct
      541 caaaggcgcc cagccgcagg atgttgcggt cggtgacggc catccgctcc atcgtccagt
      601 tgtcggcccg ctcgacgagc agcctgtcga tctcggcccg gtgcttccgc acgcccgcga
      661 tgagcgagcg accgaagtcg atcaactctt cgtcgccact gaggcggttg cagagaaatc
      721 gatcggctgc ttcgggactg cgatccgggt taaggtcgtc ctcatacagg acttgcagca
      781 cgacttcgcg tgcccggctg cgacgcgaca tgatatataa accccgaagg gataatagct
      841 tgcgaatcgc ccgtgcggcc acagggacca cccaggttcc ccgaattata ctgcgcgggg
      901 ccgaattcca gagggcaggt agcaatcacg ctccgcgtga atagcgcagc gggggctcct
      961 cgcgcggctc gtgacggctc ctaccagcac cacagccagg cttcctggca gctccagccg
     1021 caccaggtga accattcggc ggttcggcgc tgggcgcgga ggcaccagga ttccttcgca
     1081 ggctcggcca gtgtaatctc gcccgctggg ccatagtgct cgctcaactc aatggccgag
     1141 tagagtgcgg aggggtggac cttcgcgccg ttttcgatcg cgaagcggcc ggaagcattc
     1201 agtaccagac tcgtcagctc cgcgcagtga atgccgtcgc ccggcttcct gcggacgtaa
     1261 ttgcggagcg aatagcgccg gccgagctgc tcgtcgagcg cggttcgcat cgcccccact
     1321 tcgttcgacg aataggcagg tgtcggacgg aacgcccaga cgcggatctg gtcgtcggca
     1381 tcgcgtcggc ggtttatgcg ggccaactcg gcgtaataca cgtccagcgt gacgcggcga
     1441 acctcgcccg gcgtggcttc atagacccag cacgcgtcgg gatcgctgaa cacgagtgcc
     1501 acatgcccga tcttgccctt ggtagagaac tcgacgacgc tgttgcagtt ctccaaaaac
     1561 agcagcgtgc cgtcatccag cgggctggcc gagagcgaca gcgtcgccca taagagaatc
     1621 gaatgcatca ttcctccgca cgggccggca ggtcattgtc gcctttcgct ccgcgaaaga
     1681 gcgttcgttc gcggagcgaa agacgacatt ccggcggcgg cccctgtgaa agctattcgt
     1741 ccctggcctg ccattcttcg ccgggagtgg cggtcatggc aagtggcgcg ggggcgtcgg
     1801 aggcgttaaa ctgcgccctg gtttgacctt acacccatcg ccgcgcggcc tgacggggag
     1861 gctcacgcct cccgctcgcc aaactcctga ttcctgactc ctgacccttg caatgaaatc
     1921 tgccctcgcc gttgccgccc atcccgacga tatcgagttt ctgatgtccg gcaccctgat
     1981 gctcctccgc aaagccgggt acgacatcca ttattggaac ctggccaatg gctgctgcgg
     2041 gtcgagccaa ca
//
LOCUS       DASUGB010000155         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_295989, whole genome shotgun sequence.
ACCESSION   DASUGB010000155 DASUGB010000000
VERSION     DASUGB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295989"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFV87_03045"
     CDS             <1..192
                     /locus_tag="VFV87_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102757.1"
                     /translation="QEGEATGQIKVRLLDRRCNWQWWTDHNFKTAPPDAVLHFSGMVD
                     YAKRLEAMRAWSESATVRS"
     gene            162..719
                     /locus_tag="VFV87_03050"
     CDS             162..719
                     /locus_tag="VFV87_03050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102758.1"
                     /translation="MERISDRTELARRLPGGGIEAELGVCAGGYARVLLREAWPKRLY
                     LIDLWQADVMGYTATEARQRAFAAFEGEEAVRLVVSDTVAWLAARRPSSLDWCYIDSD
                     HSEPLTTRELCQSLRVVKAGGWICGHDYGPFSPGVVNAVNRFCELHGQRLTYLTDEPE
                     APPRKAAPWMPSRAACNSFAIVVEK"
     gene            716..1201
                     /locus_tag="VFV87_03055"
     CDS             716..1201
                     /locus_tag="VFV87_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102759.1"
                     /translation="MIVLPQHRTLILTPGKCGSTSLHEAFPLQLIGPQGPWRRLGHFN
                     FRDSIGKHTMLVPYDLRDYAVLIVVRHADSYVRSLWQHWQRHCERLDFETFLAKRCEW
                     RERCEPGPWFYWWDVADYLAEAPRRRDLVPLAELNETIERLTGVKLGRLNRSPGVDPQ
                     R"
     gene            complement(1342..2187)
                     /locus_tag="VFV87_03060"
     CDS             complement(1342..2187)
                     /locus_tag="VFV87_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5102760.1"
                     /translation="MRRRGFTLVEILVVLAIIAMLVALTATASMWAVNAARRAAISVE
                     VGQLRDAVEAYKQAKGDYPPNFRDYQAFIRHVRTCYPKCDVNHLDTFVGAVWPGYSLS
                     SPPPANTMPQLDEGESLVFWLQGTRNDPLRPFGGSAELVKYYSFDSRFLLDSDGDGQV
                     SFRARYAKDTFYLYMDSRSYDELTAVSGAAFVNNPAHAEGVTANGTRPYFTDAASDAL
                     PGTNYVNPTTFQIVCAGQDGEFGADTSPPTQKQFPGFRNGTSADRDNVTSFSEGKTLD
                     DNIPD"
     gene            complement(2315..2785)
                     /locus_tag="VFV87_03065"
     CDS             complement(2315..2785)
                     /locus_tag="VFV87_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102761.1"
                     /translation="MNRLPPLVQGAIGLAGVMAIGLCCGGFGYLLGRMTSPATPAAKD
                     EVQLRATMAEADRLIAEGRAAADALRPDPDYQAGVALLAEKCPLSVWRQLNVPDRYAI
                     CEEAAKRRRPRADVDFWASEYFNAIERFNYTAKPETDGSVTVWFDIIDRLRPPQ"
     gene            complement(2874..>3116)
                     /locus_tag="VFV87_03070"
     CDS             complement(2874..>3116)
                     /locus_tag="VFV87_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102762.1"
                     /translation="ADPFPLPATFLVTWPDTDHETAALVYELRWRMLRAKLPARDFQA
                     LGRDLIDPILDDPQLSPDEKRARLRHLLELVPAPPE"
BASE COUNT          550 a         1008 c         1040 g          518 t
ORIGIN      
        1 caggaagggg aggccacggg ccagatcaag gtgcgactgc tcgaccgccg ctgcaactgg
       61 cagtggtgga cggaccacaa tttcaagacc gcgccgcccg acgccgtgct gcacttcagc
      121 ggcatggtcg attacgcgaa gcggctggag gcgatgaggg catggagcga atcagcgacc
      181 gtacggagct agcccgccgg cttcccggcg gaggcattga ggccgagctc ggcgtctgcg
      241 ccggcggcta tgcccgcgtg ctgctgcgcg aggcgtggcc caagcggctc tatctgatcg
      301 acctatggca ggccgacgtg atgggttaca cggccacgga ggcgcggcag cgggcatttg
      361 ccgcattcga gggcgaggag gccgtgcggc tggtggtcag cgataccgtc gcctggctgg
      421 ccgcccggcg gcccagcagc ctcgactggt gctatatcga cagcgatcac agcgagccac
      481 tgacgactcg cgaactctgc cagtcgctgc gcgtggtgaa ggccggcggc tggatttgcg
      541 gccacgacta tggtccgttc tcgcccggcg tcgtcaacgc cgtcaaccgc ttctgcgagc
      601 tgcacggcca gcgcctcacg tacctgaccg acgagccgga agcgccgccg cggaaggccg
      661 cgccgtggat gccgagccgg gcggcgtgca acagcttcgc gatcgtggtg gagaaatgat
      721 cgtcctaccg cagcatcgaa cgctgattct cacgcccgga aagtgcggca gcaccagcct
      781 gcacgaagcc ttcccgctgc agctcatcgg cccgcagggt ccgtggcggc ggctgggaca
      841 cttcaacttt cgcgattcga tcggcaaaca cacgatgctc gtcccgtacg acctgcgaga
      901 ctacgccgtc ctgatcgtcg tgcgccacgc cgacagctac gtccggtccc tctggcagca
      961 ctggcagcgg cactgcgagc ggctcgactt tgagactttc ctggcgaagc gctgcgagtg
     1021 gcgcgagagg tgcgaacccg gaccgtggtt ctactggtgg gacgtggcgg actatctggc
     1081 cgaggccccg cggcggcgcg accttgttcc gctggcggag ttgaacgaga cgatcgagcg
     1141 gctgacgggg gtcaagctgg gccggctcaa tcgtagccct ggagtcgatc cgcaacggtg
     1201 agagccgtct gcacgaccgg ctccggcaga atcgcagcca gttcctcctg gtaaattcga
     1261 ccaatcaccg cagccgtgac gatcgacgca gcaaggccga ggacagtacc gacggcggca
     1321 gacgcacaca gccgctttac cttaatctgg aatgttgtca tccagagttt tgccttcgct
     1381 gaaactggta acgttgtccc gatccgcgct cgtaccgttg cggaagccag ggaattgctt
     1441 ttgcgtgggc ggggaggtgt cggccccgaa ctcgccatct tgccccgcgc agacgatttg
     1501 gaacgtcgtc gggttgacgt agttggtacc gggcagtgcg tcgctggcgg catccgtaaa
     1561 gtagggccgc gtgccgttcg ctgtcacgcc ttcggcgtgg gccgggttgt tgacgaacgc
     1621 cgcaccactc acggccgtca gctcgtcgta agagcgggaa tccatataga ggtagaacgt
     1681 gtccttcgcg tagcgagcgc ggaaggagac ctgcccgtcg ccgtcagagt ccagcagaaa
     1741 gcgactgtca aacgagtagt acttcaccag ttcggccgag ccgccaaagg gccggagcgg
     1801 atcgtttcgc gtgccttgca gccagaatac cagcgattcg ccctcgtcca attgaggcat
     1861 cgtgttcgcc ggaggtggac tggagaggct gtatcccggc cacaccgccc caacgaacgt
     1921 atcgaggtgg ttcacgtcgc acttggggta gcacgttcgc acgtgccgga tgaacgcctg
     1981 atagtcgcgg aagttgggcg ggtaatcccc cttcgcctgc ttgtacgcct cgaccgcatc
     2041 gcggagctgg ccgacttcaa cggagattgc cgcccgccgc gcggcgttca cggcccacat
     2101 cgaagccgtc gccgtcaacg cgaccagcat ggcgatgatc gccagcacga ccagaatctc
     2161 gactagagtg aatccgcgcc gccgcatggg tcacctccaa agatttgacg ctgaacctcc
     2221 gcagggtatt cgccgccggg gagccttcat cgtcgccccg actgccagga aaagcaagaa
     2281 tggcgcgggg cggcttgcgg caacccgcag gcggctactg cggcggtcgc agacgatcga
     2341 ttatgtcgaa ccacaccgtg accgagccat cggtctccgg tttcgccgtg tagttaaacc
     2401 gctcgatcgc gttgaagtac tcgctggccc agaagtccac gtcggcgcga ggtcgccggc
     2461 gcttggcggc ctcctcgcag atcgcgtacc tgtcgggcac attgagctgc ctccaaacgg
     2521 agagggggca cttctcggcg agcagggcga cgcccgcttg gtaatcggga tcgggccgca
     2581 gggcgtccgc ggccgcccgc ccctccgcga tcagacggtc ggcctctgcc atagtcgccc
     2641 ggagttggac ctcgtccttc gcggccggcg ttgctggcga agtcattcgc cccagcagat
     2701 agccaaatcc gccacagcaa aggccgatgg ccatcacgcc ggccaggccg atcgcgcctt
     2761 ggaccaaggg cggcaggcgg ttcatcgcag cctccgctca gagaagagga acccgtacgc
     2821 tccggcgacc agtctgccgc ccgccgcggc ggctgtcaat cttccgcgcc gtgtcactct
     2881 ggcggagcag gtactagctc cagaaggtgg cggaggcgcg cccgtttttc gtcgggagac
     2941 aactgcggat catcgaggat cgggtcgatc aggtcccggc ccaacgcctg aaaatcgcga
     3001 gccgggagtt tggcgcgcag cattcgccag cgcagctcat acacgagggc cgccgtctcg
     3061 tggtcggtgt cgggccaggt caccagaaaa gtcgccggca gcgggaaggg gtccgc
//
LOCUS       DASUGB010000156         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1268724, whole genome shotgun sequence.
ACCESSION   DASUGB010000156 DASUGB010000000
VERSION     DASUGB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268724"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFV87_03075"
     CDS             <1..642
                     /locus_tag="VFV87_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102763.1"
                     /translation="KLNGDDLPWDVNRVVGVERVAVEAQASLGQKTWHYSLDFNEKCA
                     NVEYLLDFLRQLQERAPAAMARVHYIEQPTHRDLKRHPENKMHKAAAIKPVVIDESLV
                     DLESLELAREQGYSGVALKACKGHTEALLMGAAAQKYGMFLCVQDLSCPGYSFLHSAS
                     IAARIPTIQAIEGNSRQYCPAGNRTWGEIYPSMFHITDGTVGTGVLTGKGLGF"
     gene            complement(639..1922)
                     /gene="hemL"
                     /locus_tag="VFV87_03080"
     CDS             complement(639..1922)
                     /gene="hemL"
                     /locus_tag="VFV87_03080"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5102764.1"
                     /translation="MNRSRSQQIFARAQQLMPGGVNSPARAFGGVGGEPVVFERAEGA
                     YLYDVDGNRYIDYIGSWGPMILGHQHPKVVAAVHAAVDRAFSFGAPTPAENELAELIA
                     AAVPSVEMVRLVNSGTEATMSAIRLARGFTGRDVIVKFAGNYHGHVDSLLVAAGSSAA
                     TLGVPNSPGVTGGTTKDTIIARYNDAAGLERIFAEHGPRIAGAIFEPIVGNMGTVVPT
                     PEFLRALRELTRKHGALLICDEVMTGFRVAYGGAQALLKIDPDVTTLGKIVGGGMPIG
                     AYGGRREILQHVLPAGKVFQAGTLSGNPVATAAGIATLKELRDNPPYEQLERRGAQLE
                     AGLHAAAEKAGVPHAITRVGSMLTLFFNKVRPADWESASQSDTARYAKYFWSLLDQGV
                     YMPCSQYEALFVSAAHSEADIQATIDAAANALTVR"
BASE COUNT          360 a          669 c          690 g          372 t
ORIGIN      
        1 aagctcaacg gcgacgatct gccgtgggat gtgaatcgcg tcgtcggcgt cgagcgcgtg
       61 gcggtggagg cgcaggcttc gctcggccag aagacgtggc attactcgct cgacttcaat
      121 gagaagtgcg cgaacgtcga atacctgctc gatttcctgc ggcagcttca ggagcgagcc
      181 ccggcggcga tggcccgcgt gcattacatc gaacagccga cgcaccgcga cctgaaaagg
      241 cacccggaaa acaagatgca caaggccgcc gcgatcaagc cggtggtgat cgacgaatcg
      301 ctggtcgatt tagagagcct ggagctggcc cgcgagcaag gctacagcgg cgtggcgctg
      361 aaagcgtgca aagggcacac ggaggcgctg ctgatgggtg cagcggccca aaaatacggt
      421 atgttcctct gcgttcagga cttgagctgc cccggctatt cgttcctgca ttcggccagc
      481 atcgccgccc gcattcccac gattcaggcg atcgagggca acagccggca gtattgcccc
      541 gcgggcaacc gcacctgggg cgagatctat ccgagcatgt tccacatcac cgacggtacg
      601 gtcgggacgg gcgtgctgac gggcaagggg ctcgggtttt agcgcaccgt caaggcattc
      661 gctgcggcat cgattgtggc ctggatgtcg gcttcgctgt gggcggcgga gacgaagagt
      721 gcttcgtatt ggctgcaggg catgtacacg ccctggtcga ggagcgacca gaagtacttg
      781 gcgtagcggg cggtgtcgct ttggctggcg ctttcccaat cggccggcct gaccttgttg
      841 aagaacaagg tcagcatgct gccgacgcgg gtaatcgcgt gcggaactcc cgccttctcg
      901 gcagcggcgt gcaggcccgc ttcgagttgg gcgccgcgtc gctcgagttg ttcgtagggc
      961 gggttatcgc gcagctcctt cagcgtggcg atgcccgcgg cggtggctac cggattgccg
     1021 ctgagcgtgc cggcctgaaa gaccttgccc gctggcagca cgtgctgcag gatctcgcgc
     1081 cggccgccat acgcgccgat cggcatcccc ccgccgacga tcttgcccag cgtggtgaca
     1141 tccggatcga tcttcagcag cgcctgcgcc ccgccatacg ccacgcgaaa gccggtcatc
     1201 acttcgtcgc agatcaagag cgcgccgtgc ttgcgggtca gctcgcggag cgcccgcaaa
     1261 aactccggcg tgggcaccac cgtgcccata ttgccgacga tcggctcgaa gattgcaccg
     1321 gcgatgcgcg ggccatgctc ggcgaaaatg cgctctaggc ccgcggcgtc gttgtagcgg
     1381 gcgatgatcg tgtccttcgt cgtgcccccc gtcacgccgg gcgaattggg cacgcccaac
     1441 gtcgccgccg agctgcccgc tgcgaccagc aggctatcga cgtggccgtg atagttgccg
     1501 gcgaacttga caatcacgtc ccgccccgtg aacccgcggg ccaggcgaat cgcgctcatc
     1561 gtcgcctcgg tcccggaatt cacgaggcgg accatctcga ccgacggcac ggctgccgcg
     1621 attaattcgg ccagctcgtt ttcagccggt gtcggcgcgc cgaagctgaa cgcgcggtcc
     1681 acggcggcgt ggacggcggc aacgaccttg gggtgctgat gccccaggat catcgggccc
     1741 catgagccga tgtagtcgat gtagcggttg ccatcgacgt cgtacagata cgctccctcg
     1801 gcccgctcga agacgaccgg ctcgcccccg acgccgccaa acgcccgcgc ggggctgttc
     1861 acgccgccgg gcatcaactg ctgagcgcgg gcgaatatct gctgactacg gctgcggttc
     1921 attgttttcg tggggtaggc gtctcgcctg cccgtcagtt gctagcggta agtgctggcg
     1981 atccgcgcga gactacgact aatatcaatg caagtgtcaa ttgagtccag ccggtgaatc
     2041 acgtaggatt cgccgccttt ccgaatatct ggcaggcagg atgcctgccc c
//
LOCUS       DASUGB010000157         7756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_852916, whole genome shotgun sequence.
ACCESSION   DASUGB010000157 DASUGB010000000
VERSION     DASUGB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852916"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..479
                     /locus_tag="VFV87_03085"
     CDS             270..479
                     /locus_tag="VFV87_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102765.1"
                     /translation="MKLDSIRWISLVAVAGALAAGPGCEARVTTDQPPPKVEIESEPK
                     PNVDVNIEHKPRGLDVDVNVDKSPP"
     gene            complement(496..729)
                     /locus_tag="VFV87_03090"
     CDS             complement(496..729)
                     /locus_tag="VFV87_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5102766.1"
                     /translation="MDSNLPTDLHLEREVRAALSANWCHRSLRSLAVWVDDGVVTLRG
                     QTGSFYEKQIGLSSVQKVSGISSIVDEVSVLGE"
     gene            complement(924..1142)
                     /locus_tag="VFV87_03095"
     CDS             complement(924..1142)
                     /locus_tag="VFV87_03095"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX5102767.1"
                     /translation="MQSLLASNRYSINFVAKTIMSLLGTILLIFLILLLLGSVPAWPY
                     SRGWGYGPTGGLGLILVIVLILVLLGRI"
     gene            1294..1971
                     /locus_tag="VFV87_03100"
     CDS             1294..1971
                     /locus_tag="VFV87_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102768.1"
                     /translation="MVLAFGSSIAIAEEPNLPRPYDGIQAGYDAFQLAEERRRADIAE
                     QLFLNDQLKYWSGLPTSRGETIYYGAPYATAGAYYGSAAPVLPPANLDYGYAYGRWAA
                     NRPILGRGWNGPRTVFEPWPYIPGDIYGYTYYSPMRQPVGQQQVQTGENRWESHPVYD
                     PPLPEYRPLPPVDSRYLDDTPFASPRSAPEMRALPPPAIDPPSFGAPGTDAELPPTPP
                     KRRPREY"
     gene            2112..2184
                     /locus_tag="VFV87_03105"
     tRNA            2112..2184
                     /locus_tag="VFV87_03105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Lys,seq:ctt)
     gene            complement(2312..3520)
                     /gene="ilvA"
                     /locus_tag="VFV87_03110"
     CDS             complement(2312..3520)
                     /gene="ilvA"
                     /locus_tag="VFV87_03110"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX5102769.1"
                     /translation="MPVTLDDIRAAKDRIAGAVAVSPCPESPALSDLTGCRVFCKLDS
                     LQRTGSFKERGARNALLLLPQEQQKCGVIAASAGNHALALACHGRGLGIPVTVVMPRF
                     APLIKVATCRRFAANVVLHGSDFAEAKAKADELASAHGLTYIHGYDDPAVIAGQGTIG
                     LELLEQVPGLEAVVVPVGGAGLIAGIALAIKSLQPTVQIIGVEDEVMPSFTCALEAGQ
                     SVAVPARPTLADGLATTRVGVNALAIAQGRVDRAVTVSEELIALSILRLIELEKSVVE
                     GAAAAPLAALLSGKLPELRGKCVALILAGGNIDPLILGRVIDVGMAADGRLARLVATI
                     SDRPGGLAHLARVVAESGASIQQITHDRVFSGPDVSSVRVICDVETRDRNHLDELRAA
                     LQRSGIELRT"
     gene            complement(3714..4748)
                     /locus_tag="VFV87_03115"
     CDS             complement(3714..4748)
                     /locus_tag="VFV87_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5102770.1"
                     /translation="MNLPPAVGSSQELDDLLSTPTPGVIESLGRLEGDLIVLGIGGKM
                     GPTLARMAARASQEAGIKRRVIGVARFSRQELPAWLKEHGIEPLACDLMDSRQLAKLP
                     DAANVIVMTALKFGSTGRPGDTWAVNCWMPANICQRYAAGRIAAFSTGNVYPLSPVVS
                     GGSQETDPLGPVGEYAASCVGRERLYDYFSRANGTKLSIVRLNYACELRYGVLVDLAR
                     QIMAGEPIDLSMGAVNVIWQGDANAMTLQSLAHAASPPFVVNVAGPETLSVRRVCEQL
                     GDLLDRPPVFTGSEGPDALLSNGSLGHKLFGYPRVPINRLCEWIADWLKRGGELLDKP
                     TKFQVRDGKF"
     gene            4917..5756
                     /locus_tag="VFV87_03120"
     CDS             4917..5756
                     /locus_tag="VFV87_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5102771.1"
                     /translation="MLGLHFLVEGITKLQDDKPFTAPFFSSARGPLAPAFKRMVWDAD
                     GLWRLNKDATVAHWDSYKNRVVRHYGFDDAQAKEADSVLKAYKDRLEWFLNDKGAAIT
                     EYHNQLHRRDENAKNDTRQLASLQVHDTRIASETKKLYVELIPPIDGLWRDLENDLNA
                     IATPDQWKRHGRLAIGKIGRGFPDSETLDAVMPYFDVVVGICLIVGLFTRPAAILAAM
                     FLATVFLSHFPPEPGPGSTYYHLVEMFALFAIAAVGGGRYLGLDYFLGLICCRGKKTG
                     ETK"
     gene            5753..7588
                     /locus_tag="VFV87_03125"
     CDS             5753..7588
                     /locus_tag="VFV87_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5102772.1"
                     /translation="MTPLTPEERETGKENFYSAVSTFDQSPAAEKWKRRDFLKTVMGV
                     GAAAATAGMTTGCHYFGYSPIGDPLRVAVIGTGDEGSVLIGAINPAYVNVVAICDIRP
                     YNVHRAFQGDWSSDAGIAARPGLIAKYGYADEKAAKDKIQVETEYKKVLENPDVEAVI
                     IALPLHLHAKVAVDAMLAGKHVLTEKLMAHNVAQCKVMGRVAAAHNKVLAVGHQRHYS
                     VLYDNAVNMLKWGLLGELHHIRAQWHRGNLPGHDSWQQPLPVPVEAYIDGDKKMVNFV
                     QDKRKDLESALKKEKDPLKARLLQKQVAQWLAWEEDAQIEAEKYGYENFNLGDGRTRT
                     ALEELCRWRLFKRTGGGLMAELGSHQLDAASIFCSALRADGKKAHPLSVHAVGGRHIF
                     PLDRHAEDHVYCTFEFPGPGYEADFPGYFDPAMNYPDPKKGIPNYNDDPNKKIVVTYS
                     SIMGNGWGGYGETVMGTKGTLILDREQEVLLFKDSDTSTKVGVKEDKGGPTLDTQASG
                     KGPSLARAAAESGPVSRGYTEEIEHWAWCIRNQSSENQPRCRPEVALGDAVIALTCNV
                     AIDNSINRKAGYIQFEEEWYDINSDETPDESNLKDENESLGAGLS"
BASE COUNT         1528 a         2447 c         2427 g         1354 t
ORIGIN      
        1 cagcgcgacg tagaggatgc agcccgcgag gctcagaaaa gcgtaactca ggagcaacgc
       61 gagcttcaag atgcggcccg cgaagaacag gagaaggtcc tccaggaaaa gcgtgatttg
      121 gaagacgccg aacgccggga aacggcagaa ccccaatctt agctgccgag acagagagtc
      181 aatcggcggg ccggcagcgg aagcttgggc gttgccggcc cgctttgcta tggcacggca
      241 catatcgcac aagggtgagg agttactgta tgaagctcga ttcgatccgg tggatctcat
      301 tggttgccgt tgcgggcgcg ctggctgcag gaccggggtg tgaggcccgt gtcactaccg
      361 accaaccgcc acccaaagtt gaaatcgaga gcgagcccaa acccaacgtg gacgtgaaca
      421 tcgaacataa gccgaggggc ctggacgtag atgtcaatgt cgataagtcg ccgccctagc
      481 cgaactcctc gatcgctact cgcccaggac ggaaacctcg tccacgatgc tggatatccc
      541 gctcactttc tgaacggaac ttaatccgat ctgcttttca taaaagctgc cagtctgacc
      601 gcgaagcgta acaacgccgt catcgaccca gactgccagg ctgcgaaggg agcggtggca
      661 ccagtttgcg gataaggcag cccgcacctc ccgttccaaa tgcaagtcgg tcggcaggtt
      721 ggagtccaat tcaggcaatc ctgttgaatc cattgagaac tctcgttttc atgagggatc
      781 ggatcgagac gcggccggtc gagcttgcaa ctagagtgcc gggccgaaat ggtcagtttc
      841 gctgagctag atgcaactgc cgtcagccgc gtagcgacat tggactggtc attttgtcgc
      901 gttcgacgag gggaacgaga gaattagatt cgtcctaaga gcaccaaaat caggacgatc
      961 acaagaatca gccccagacc gccagtcggg ccatagcccc aaccgcgact ataaggccag
     1021 gcaggtaccg atcccagcaa gagcagaatc aagaaaatga gtaaaatggt tcccaggagt
     1081 gacatgattg ttttcgccac gaagttgatg gaataacggt tgctggccag tagcgattgc
     1141 attcggcgtg ccgtccagtc gaaatcttaa gcgatgtcga ccgtggcggc gtgcagcttc
     1201 agtacaataa ttgccgcagt tgcctgacag agttcaaatg gaccgacccc acggaggcag
     1261 aaggtgagac actcgatttc cgtcgcgagg gctatggttc tcgcatttgg ctcttccatt
     1321 gccatagccg aagagcccaa tctcccgcgt ccgtacgatg gcattcaagc gggatatgac
     1381 gcctttcaat tggccgagga gcgccggcgc gcggacattg ctgagcagct cttcctcaac
     1441 gatcaattga agtattggtc gggtctcccc accagccgcg gcgagacgat ctattacggc
     1501 gccccctatg ccactgctgg cgcgtactac ggcagcgcgg cgccggtgct gccgccagcg
     1561 aatttggatt acggctacgc ctatggtcgc tgggccgcga atcggccgat tctggggcgt
     1621 ggctggaacg gtccgcgcac tgtgtttgag ccttggcctt acattccggg cgacatctac
     1681 ggctacacgt actattcacc gatgcggcag cccgtcggtc agcagcaagt ccaaacgggc
     1741 gagaaccgct gggagtcgca tcccgtttat gacccaccgc tgccggagta tcgcccactg
     1801 cctcccgtcg attctcggta cctggatgat actcccttcg catcgccgcg gtcggctcct
     1861 gaaatgcggg ccttgccgcc gccagcgatt gatccgccct cattcggcgc gcctggaacc
     1921 gacgccgagc taccgcccac accacccaaa cgacgtccgc gggaatattg aggatatccc
     1981 acgccatctc gcgagtctcg ccatccgccg cgaacccggc actcaacggc agcgtccaga
     2041 ctgagcggcc cttgtgggcc atcgtcaatc tgttacgctc attagctcgt tgcaattgat
     2101 cggcaaatta cgccccgcta gctcaattgg cagagcatct gactcttaat cagagggtta
     2161 atggttcgag tccattgcgg ggcacttttt gcttctcgcc aagtctgtcg ccgagcgcga
     2221 cgttgagaaa actcccggct gcattgggat gtgaagccaa atcgacgtgt ttctggtgcg
     2281 tactgcgccg ccttcgatcg acgccatctg cttaggttcg caattcgatg ccgctgcgct
     2341 gaagggcggc tcgcagctcg tctagatgat tgcggtcgcg ggtttcgacg tcgcaaatca
     2401 cgcggacgct gctgacatcc gggccggaga agacgcggtc gtgcgtgatc tgctggatgc
     2461 tggcgccgct ctcggcgacc acgcgggcca gatgggccag gccgccgggc cggtcgctga
     2521 tcgtcgcaac cagccgggcc agccggccgt cggcggccat gccgacgtcg atcacgcggc
     2581 cgagaatcag cgggtcgatg ttgcccccgg ccagaatgag cgcgacgcat ttgccgcgga
     2641 gctccggcag cttgccgctt aagagggcgg cgagcggagc ggcggctgcc ccttcgacga
     2701 cgctcttttc cagctcgatc agccgcagga tggagagggc gatcagctct tccgagacgg
     2761 tcacggcacg atcgacgcgg ccttgggcta tggccagggc attgactccg acgcgggtcg
     2821 tcgccaggcc gtcggctaga gtcgggcggg cgggcacggc gaccgattgg ccggcttcca
     2881 gggcgcaggt aaagctgggc atcacttcgt cttcgacgcc gatgatctgc acggtcggct
     2941 gcagcgattt gatcgccaga gcgatgccgg cgatcagacc tgcgccgcca acaggcacca
     3001 caaccgcctc cagcccaggc acttgctcga gcaattccag gccgatcgtt ccctggccgg
     3061 cgatcacggc gggatcgtcg tagccgtgga tgtaggtcag gccgtgggcc gacgccaatt
     3121 cgtcggcttt ggctttcgct tcggcaaagt cgctgccgtg cagcacgacg ttggcggcga
     3181 agcggcggca ggtggcgact ttgatgagtg gggcaaaccg cggcatgacg acagtgacag
     3241 ggatgcccag cccgcggccg tggcaagcga gggccagggc gtgattgcca gccgaggcgg
     3301 cgatgacgcc gcatttttgc tgctcttgcg gcaaaaggag caaggcgttg cgggccccgc
     3361 gctctttgaa gctgcccgtc cgctgcagcg aatcgagctt gcagaacacg cggcagccgg
     3421 tgaggtccga gagggcgggc gattccgggc agggcgagac ggcgacggcg ccggcgatgc
     3481 gatctttcgc ggcgcgaata tcatcgaggg taactggcat tgtccccatc gtagcccgca
     3541 gcgccagcga gggtaatgcg ggggaatcgt agcccgtcgc gcaagcgagg ttggtgccgg
     3601 tgaatcgtgg cccgcagcgc aagagggcaa gcgttggttt ccatcaagag aggcgctgcg
     3661 tttttgcggt ctggccacgt cgtttaccct cgcttgcgct acgggctact acgtcaaaac
     3721 ttgccgtcgc ggacttgaaa cttcgtcggc ttgtcgagca gctcgccgcc gcgtttcagc
     3781 cagtcggcaa tccactcgca aagccgattg atgggcaccc gcggatagcc gaagagcttg
     3841 tggccgaggc tgccattgct gagcaaggcg tccggccctt cgctgccggt gaagacgggc
     3901 ggcctatcga gcagatcgcc aagctgctcg cagacgcggc gaacggagag cgtctccggg
     3961 ccggcgacgt tgacgacgaa tggcggcgaa gcggcgtggg ccagcgactg cagggtcatc
     4021 gcgttggcgt cgccctgcca gatgacattg acggccccca tcgatagatc gatcggctca
     4081 ccggccataa tctgccgggc cagatcgacc agcacgccgt atcgcagctc gcaggcgtag
     4141 ttcaggcgca cgatcgacag cttcgtgccg ttggcccggc tgaagtagtc gtagagccgc
     4201 tcgcggccga cgcagctcgc ggcatattcg ccaactgggc caagcggatc tgtttcctgc
     4261 gatccgccac tgacgaccgg cgacagcgga tagacgttgc ccgtcgagaa ggccgcaatg
     4321 cggccggccg catagcgctg gcagatgttg gccggcatcc agcagttgac ggcccaggta
     4381 tctcccggcc gccccgtcga gccgaacttg agggcggtca tcacgatcac attcgccgcg
     4441 tccggcagct tcgcgagctg ccgtgagtcc atcaggtcgc acgccagcgg ctcgatgccg
     4501 tgctctttca accaggcggg cagctcttgc cggctgaagc gggccacgcc aatcacccgc
     4561 cgcttgatac ccgcctcctg cgaggcccgc gcggccatcc gggccagcgt cggccccatc
     4621 ttgccgccga tgcccagaac gatcagatcc ccctccagcc gccccagtga ctcaatgaca
     4681 ccgggcgtcg gcgtgctgag caggtcgtcc aattcctgcg aagatccgac cgcgggtggt
     4741 agattcatgg tgctcctaac tcggtccaag cggttggcga atccgcaatt cctggcaata
     4801 ataagggact tgcccgcccc ctccctagac aacctctccc ggagactcgt ccgctgtctg
     4861 cctcatcgta ccgcatcggc tggctggctg ccgccggaat tgttgccctg cggctggtgc
     4921 tggggctcca ttttttggtg gaaggaatca ccaagctcca ggacgacaag ccgttcaccg
     4981 ctccgttttt cagctcggcc cgcggtccgc ttgcgcccgc cttcaagcgg atggtttggg
     5041 acgccgacgg cctgtggcgg ctcaataaag acgccaccgt ggcccactgg gacagctaca
     5101 agaaccgcgt cgttcgccac tacggctttg atgacgccca agccaaggaa gcggattccg
     5161 ttctgaaggc ctacaaagac cggctggagt ggttcctgaa cgacaagggc gcggcgatca
     5221 ccgaatacca caaccagctc caccgccgcg acgaaaacgc gaagaacgac acgcggcagc
     5281 tcgcctcgct tcaggtccac gacacccgaa tcgcctccga aaccaaaaaa ctctacgtcg
     5341 agctgatccc gccaatcgat ggcctgtggc gagacctcga aaacgacctc aacgccatcg
     5401 ccacgcccga ccagtggaag cggcacggcc gcctcgccat cggcaagatc ggccggggat
     5461 tcccggatag cgaaaccctg gacgccgtga tgccgtactt cgatgtagtg gtcgggattt
     5521 gcctgatcgt gggcctgttc acgcggccgg cggcgatcct ggcggctatg ttcctggcga
     5581 ccgtcttcct ctcgcacttt ccgccggagc ccggtcccgg ctcgacgtat taccacctgg
     5641 ttgaaatgtt cgccctgttc gcgatcgcag cggtcggcgg cgggcgctat ctcggacttg
     5701 attacttcct cggcctgatc tgctgccgcg gcaagaagac aggagaaacg aaatgacccc
     5761 gcttactccc gaagaacgcg aaaccggcaa agagaatttc tactcagccg tttcgacctt
     5821 cgatcaatcg cccgctgccg agaaatggaa gcggcgcgac ttcctcaaga ccgtcatggg
     5881 cgtcggcgcc gccgcggcca cagccggcat gaccaccggc tgccattact ttggctattc
     5941 gcccatcggc gacccgctgc gggtggcggt gatcggcacg ggggacgagg ggagcgtgct
     6001 gatcggggcg atcaatccgg cgtacgtcaa cgtcgtcgcc atctgcgaca tccggcccta
     6061 caacgtccat cgcgcgttcc aaggcgattg gtcgagcgac gccggcatcg ccgcccgtcc
     6121 gggcctgatc gccaagtacg gctatgcgga cgagaaggcc gccaaggaca agatccaggt
     6181 cgagaccgaa tacaaaaagg tcctggaaaa cccggacgtc gaggcggtga tcatcgccct
     6241 gccgctgcat ttgcacgcca aggtcgcggt cgatgcgatg ctggccggca agcacgtgct
     6301 gaccgaaaag ctgatggccc acaacgtggc ccagtgcaag gtgatgggcc gggtcgctgc
     6361 cgcccacaac aaggtgctgg ccgtcggcca ccagcggcac tatagcgtgc tctatgacaa
     6421 cgccgtgaac atgctcaagt ggggcctcct cggcgagttg caccacatcc gcgcccagtg
     6481 gcaccgcggc aatcttccgg gccacgatag ctggcagcag ccgctgcccg tgccggtcga
     6541 ggcgtatatc gacggcgaca aaaagatggt caatttcgtc caggataagc gaaaagacct
     6601 ggaatcggcc ctcaagaagg aaaaggatcc gctcaaagcg cgcctgctgc aaaagcaggt
     6661 cgcccagtgg ctcgcctggg aagaagacgc ccagatcgaa gcggaaaagt acggctacga
     6721 gaatttcaac ctgggcgacg gccgcacgcg aaccgccctg gaagagctct gccgctggcg
     6781 gctgttcaag cggacggggg gcggcctgat ggcggagctc ggcagccacc agctcgatgc
     6841 ggccagcatc ttctgcagcg ccctgcgtgc ggacggcaag aaggcccatc cgctctcggt
     6901 ccacgccgtc ggcggccggc acatcttccc gctcgaccgt catgccgagg accacgtcta
     6961 ttgcacgttt gagttccccg gcccgggtta cgaggcggat ttccccggct acttcgaccc
     7021 ggccatgaat tatcccgatc cgaagaaggg catccctaac tacaacgacg accccaataa
     7081 gaagatcgtc gtcacctatt cgtcgatcat gggcaatggc tgggggggct atggcgagac
     7141 ggtcatgggg acgaaaggga cgctgattct cgaccgcgag caggaagtgc tcctcttcaa
     7201 ggattcggat acctcgacga aggtcggcgt caaggaggat aaaggcgggc cgaccctcga
     7261 tacccaggcc agcggcaaag gcccttcgct ggcccgcgcc gccgccgaaa gcggccccgt
     7321 cagccgcggc tataccgagg aaatcgagca ctgggcctgg tgcatccgca accaatccag
     7381 cgagaaccag ccgcggtgcc ggccggaggt ggctctgggc gacgcggtga tcgccctgac
     7441 ttgcaacgtc gcgatcgaca actcgatcaa ccgcaaagcc ggctacattc agttcgagga
     7501 ggaatggtac gacatcaaca gcgacgagac ccccgacgag agcaacctca aggacgagaa
     7561 cgagagcctc ggcgcgggat tgtcctgagt agttgtggtt gtagggttca caaggccgcc
     7621 gcggccgcac tacaccagtg ctcggcctcg cgttcggtgc gggtctcccg gccggaggtc
     7681 tccccgcgcc gccggtcatg gagaccttcg gtcggccgct tggcatggtc tggagaccgt
     7741 gccaaagcgc agttga
//
LOCUS       DASUGB010000158         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_18702,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000158 DASUGB010000000
VERSION     DASUGB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18702"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1469)
                     /locus_tag="VFV87_03130"
     CDS             complement(102..1469)
                     /locus_tag="VFV87_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5102773.1"
                     /translation="MSAKTKANPPRRRPLTELMDRQPPFDLQAEIGVLGSIVLLPDVL
                     DHLRPILKTADYYDDAHSTLYRQMLELHDAGKKIDPTLLVDRLKAAGEYEAIGGAAYL
                     SKIVNAVPNAAHATYYAWIVAKHSERRRLIVAFTEGLQDTYDGDEPDEITDRLEAKLQ
                     SLGSSRESEALSISEVAAAAISEVEADTKAPCWPGCMSGLDRLDQAKGPILPGEVEVI
                     AARPGNGKTSYAAQVAIHNAERGRSVLFVSMEMRATELVKRDLCSRAGLDSRDVRSGR
                     FKAGDVQRILAARGELDGLPLRLWAPPAATLSDIRGMARSCKAKAGLQLLVIDYLGLV
                     RPEADDRRLSLYERVTKTSAGLKALARELDVPIIALVQLNREGEKEEPRLSHLRDSGA
                     IEQDADSVLFIHHPPSAKQFSGGEGVYAVGVHLIIAKHRHGERGRVSLLWHPRETRFS
                     SPTSF"
     gene            complement(1469..1753)
                     /locus_tag="VFV87_03135"
     CDS             complement(1469..1753)
                     /locus_tag="VFV87_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102774.1"
                     /translation="MHCRQATSKRQTAFRVRARDPQWLAGIDDALSAVAASQFLQGDN
                     DRNWRADIDWFLRPDSLTRILEGKYSSKSQVASLYADLDHQAAASNGRTA"
BASE COUNT          347 a          664 c          678 g          413 t
ORIGIN      
        1 cggcgtggtg cttcccggct tgttgcggct gggtcgtcgg agccggtcgt acggcgtgag
       61 caaagaggtg gggctgggca tgatcaaatt tcctttccag gtcagaaact cgtggggctg
      121 gaaaaccgcg tctcgcgcgg atgccacaag agcgacacgc ggccgcgctc gccgtggcgg
      181 tgcttggcga tgatcaggtg aacgccgacg gcgtacaccc cttcgccgcc gctgaattgt
      241 ttggccgacg gcgggtggtg aatgaacagg acggaatcgg cgtcctgctc gatcgccccg
      301 gaatcgcgca ggtggctcag ccgcggttct tccttttcgc cctcacgatt gagttggacc
      361 agggcgatga tcggcacgtc caactcgcgg gccagcgcct tcaagccagc cgatgtcttt
      421 gtgacgcgct cgtacagcga cagccggcga tcgtcggcct cgggtcggac caggcccaga
      481 tagtcgatca ccaagagctg cagcccagct tttgctttgc aagagcgggc cataccgcga
      541 atgtcgctca gcgttgccgc cggcggggcc cacaggcgga gcggcaggcc gtcgagctcg
      601 ccacgggccg cgagtattct ctggacgtca ccggccttga agcgaccgct tcgaacatcg
      661 cgggagtcga gtccggcccg gctgcacaag tcccttttga ccagttccgt cgcccgcatt
      721 tccatgctga cgaacaggac ggaccggccg cgctccgcgt tgtggatagc cacttgggcg
      781 gcatagctgg ttttcccatt cccgggccga gctgcgatga cttccacttc gcccggcaag
      841 ataggcccct tggcctggtc gagtcgatcg agtccggaca tgcagcccgg ccagcacggc
      901 gccttcgtgt cggcttcgac ttcagagatt gcggccgcgg cgacttcgga gattgacagg
      961 gcctcggatt cacgcgagct gcccagcgat tgcagcttgg cctccaggcg gtcagtgatt
     1021 tcgtcgggct catccccgtc gtatgtgtcc tgcaaaccct cagtgaatgc gacaatcagc
     1081 ctccgtcgtt cggagtgctt agcgacgatc caggcgtagt atgtcgcgtg cgcggcgttg
     1141 ggaacggcgt tgacgatctt ggacaggtag gccgcgccgc cgatggcttc gtattcgccc
     1201 gctgccttca gtcgatcgac gagcagcgtt gggtcgatct tcttgccggc atcgtgcagc
     1261 tcgagcattt gccggtagag tgtgctgtgg gcatcgtcgt aataatcggc cgttttcaag
     1321 attggccgaa gatggtcgag gacatcgggc agcaaaacga tgctgccgag cacgccgatc
     1381 tccgcctgca aatcgaacgg cggctggcgg tccatcagct ccgtcagcgg gcgacggcgc
     1441 ggcgggttag ctttggtctt tgcgctcatc aggcggtcct cccgttgctg gcagccgcct
     1501 gatgatcaag atccgcgtac agactggcca cctgcgattt gctcgaatac ttgccctcca
     1561 gaatccgcgt gagagaatcc ggtctgagaa accagtcgat gtccgcccgc cagttgcggt
     1621 cgttgtcgcc ctgcaggaac tggctcgccg cgacggcgga cagagcatcg tcgatgcctg
     1681 ccagccactg agggtcgcgt gcgcggaccc gaaaggcggt ctggcgcttc gatgtcgcct
     1741 gccgacaatg cacaatgcca gggatctcat tccagcgctc aattagcgaa tcgagtttca
     1801 gggcgctggg gtcgccgtta ggcgactgag gcgaagccga ctctccctta gggagagggc
     1861 atagggagag gatagaaggt tcccccccac cgtccccctc accgtatccc ccaccatccc
     1921 cctccccata tccggctgcg ggggatggtt gctcagagct atccccctgt cccggatatg
     1981 gttttgccgg ccgtccagcc ttgccgtcgg catgtccgcg gaggtaggcc gcgcgaacga
     2041 cgtccccatc ttcgtcgatt ggcgagtcgg gaagtgcagc gaccccggcc gggatttcca
     2101 cc
//
LOCUS       DASUGB010000159         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_991809, whole genome shotgun sequence.
ACCESSION   DASUGB010000159 DASUGB010000000
VERSION     DASUGB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991809"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFV87_03140"
     CDS             <1..1005
                     /locus_tag="VFV87_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEX5102775.1"
                     /translation="GLGGIGGGYGISEGAATPVLSLQATGGNPGPQYQIINMLRVPGV
                     QQVALKVKIAELNRSAARGFGVDVNAAINFSDSVEGSKLFLSSLLNAGNTGGTSLLTS
                     FSGDDVSIGIRYLQERGVIRLLSEPTLVTLSGRPATFIAGGEFAVPTIVGSVGLNAVT
                     TDFRAFGAIISFLPTVVDKDRVRLQVAPEFSQINNQLRVNNTPGLNVRAATTTVEMRE
                     GQTLAIAGLLEDTYKATNVGNLPGLAQIFGRRDSTRSETELIILVSPELVHPMEAEEV
                     PPLPGFDVTEPTACQFFLHGDIEGNPTQDYRSTVWPRLRKRYGMGGPAMTSGPFGHGQ
                     "
     gene            1115..1687
                     /locus_tag="VFV87_03145"
     CDS             1115..1687
                     /locus_tag="VFV87_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102776.1"
                     /translation="MKRTSLLSTLTLAALAALAGCHHRGDNCDGAGGRGSYRGPGADR
                     PFPLGQVSDSHWETQQTNAEAADFIFYDHEFRMDTRTRTDTAELTPGGKKHLQQIALR
                     LEHTPFPVVIEESPHNGRPELDQARRRTIVEQLGRLGAMNVEERVVVANAFPEGYTGI
                     ESENSYYNVLSNSFGGGAGRRFGGYGGSYR"
     gene            complement(1835..>2294)
                     /locus_tag="VFV87_03150"
     CDS             complement(1835..>2294)
                     /locus_tag="VFV87_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102777.1"
                     /translation="EFPKDLTVRITKEGDQPTKIHVKRGDQEWEVKEDKLSELPDDVR
                     PHVQKLLSRMLAPGFASAASRVLKVQPGGKVEGEIKIAPLPPLPPTPPKAPKAGAKPS
                     APAEPQVTRLHAYRVEQGGVDAKLDAIMKKLEQLTKEVDDLKDRSSSDKK"
BASE COUNT          425 a          779 c          681 g          409 t
ORIGIN      
        1 ggcctgggcg gcatcggcgg gggctatggc atctccgaag gcgcggccac gcccgtcctc
       61 agcctgcaag ccaccggcgg caatccgggg ccgcagtacc agatcatcaa catgctccgt
      121 gtgccgggcg tccagcaggt cgctttgaaa gtcaaaatcg ccgagctcaa tcgcagcgca
      181 gcccgcggct tcggcgtcga tgtcaatgcg gcgatcaact tcagcgacag cgtcgaaggc
      241 agcaagctgt tcctcagctc gctgctcaat gccggcaaca cgggcggcac atcgctgctc
      301 acctcgttca gcggcgacga cgtgagcatc ggcatccgct acttgcagga gcggggcgtg
      361 attcgcctgc tcagcgagcc gacactcgtc accttgagcg gccggccggc cacgttcatc
      421 gccggcggcg aattcgccgt cccgacgatc gtcggcagcg tcggcctcaa cgccgtcacc
      481 accgacttcc gggccttcgg cgccatcatc agcttcctgc cgaccgtcgt cgataaggac
      541 cgcgtgcggc tgcaagtcgc gcccgagttc agccagatca acaaccagct ccgcgtgaac
      601 aacacccccg gcctgaacgt gcgggccgcc acgacgaccg tcgaaatgcg cgaagggcag
      661 acgctggcga tcgccggctt gctcgaagac acctacaaag cgacgaatgt cggcaacctg
      721 cccggattgg cccagatttt tggccgccgc gattcgactc gcagcgagac cgagctgatc
      781 atcctggtca gccccgagct ggtccatccg atggaagccg aggaagtgcc gccgctcccc
      841 ggcttcgacg tgaccgagcc gacggcctgc cagttcttcc tgcacggcga catcgaaggc
      901 aatccgaccc aggattaccg cagcaccgtc tggccgcggc tccgcaaacg ttacggcatg
      961 ggcggtcccg ccatgaccag cggcccgttt gggcacggac agtaaaggaa ttatgaaaga
     1021 tgaattatga atggtcttgc gttattactt cataattcag cattcataat tcacaattta
     1081 gaagaccctc catcccgcac ggaggcggac atttatgaaa cggacttcct tgcttagcac
     1141 gctcacactc gcggccctcg ccgccctcgc cggctgccat catcggggcg acaactgcga
     1201 tggcgccggt ggccgcggca gctatcgcgg ccccggcgcc gaccggccgt ttccgctggg
     1261 gcaggtcagc gactcccact gggaaacgca gcagaccaac gccgaagcgg ccgacttcat
     1321 cttctacgat catgagttcc gcatggacac gcggacgcgg accgacacgg ccgagctgac
     1381 gcccggcggc aagaagcact tgcagcagat cgccctccgc ctcgaacaca cgccgttccc
     1441 ggtcgtgatc gaagaaagcc cgcacaacgg ccggcccgag ctcgatcagg cgcgccggcg
     1501 gaccatcgtg gaacagcttg gccgcttggg cgcgatgaat gtcgaagagc gggtcgtcgt
     1561 cgccaacgcc ttccccgaag gctacaccgg catcgagtcc gaaaactcgt actacaacgt
     1621 cctcagcaac tccttcggcg gcggcgccgg ccgccgcttc ggcggctacg gcggctccta
     1681 ccgctaagat cgcctattta gccgggagca aacctgctcc cggttgggct gttcaacaca
     1741 attagccggg agcaaacctg ctcccggtgc gacgcaactt cgtcttctac gaaagaaaac
     1801 cgggaccagg tttggtcccg gctgttggga agtgctactt cttatcgctg gacgagcgat
     1861 ccttgaggtc gtccacctcc ttcgtaagct gctcgagctt cttcatgatc gcgtcgagct
     1921 tggcatcgac gccgccttgc tcgacccgat aagcgtgcaa gcgtgtaacc tgcggctctg
     1981 caggtgcgct gggctttgct cccgccttgg gtgccttggg cggcgtcggc gggagcggcg
     2041 gcaaaggagc aatctttatt tcgccctcga ccttgccacc aggctggacc ttgagaaccc
     2101 ggctggcagc cgaagcaaag ccaggcgcga gcatccggct cagcagcttc tgcacatgcg
     2161 gccgcacgtc gtcgggcagc tcgctcagtt tgtcctcctt gacctcccat tcctggtcgc
     2221 cgcgtttgac gtggatcttc gtcggctgat cgccttcttt ggtgatccgc acggtcaggt
     2281 ccttgggaaa ttca
//
LOCUS       DASUGB010000160         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1548075, whole genome shotgun sequence.
ACCESSION   DASUGB010000160 DASUGB010000000
VERSION     DASUGB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548075"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1748
                     /gene="pckA"
                     /locus_tag="VFV87_03155"
     CDS             183..1748
                     /gene="pckA"
                     /locus_tag="VFV87_03155"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX5102778.1"
                     /translation="MTEHGLRNWDVAYWNLGTAKLVEAAIQRHEGELANTGALVVRTG
                     HFTGRSPKDKYIVRDAGTENTVAWGSVNQPMSPEKFTGLYDKLLSFWQGRTLFVQDCL
                     AGADPKHQIRVRVITETAWHSLFGRQLLIRTAIEELQDQVPDFTIMFAPTFQARPESD
                     GTNSGTVIAINFTERLVLIAGTMYAGELKKSVFTILNYLLPGNDSLPMHCSANIGPKG
                     DVALFFGLSGTGKTTLSADASRGLIGDDEHGWSDDGVFNFEGGCYAKCIHLSQKHEPE
                     IWNAIRFGAVLENVKLDVNRRNCDYDSDEFTENTRAAYRIEFIANAVTPSVGGHPKNI
                     LFLAADAFGVLPPISRLTPEQAMYHFLSGYTAKLAGTERGLGKEPSATFSACFGEVFL
                     PRPAHVYAELLGKKMREHNSRCWLVNTGWVGGAFGTGSRMSLPYTRAIVNAAIAGQLD
                     DVSTVTHPVFGVAMPTSCPGVPSELLDPRSQWADKAAYDAAARDLAGRFQKNFAKFGT
                     IDSAIANAGPKAG"
     gene            1857..>2328
                     /locus_tag="VFV87_03160"
     CDS             1857..>2328
                     /locus_tag="VFV87_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102779.1"
                     /translation="MSYLEYVTVDHESDDPMPELARTNGFSESDLALNRAGKISDPQR
                     SKLFGRALRPVKYPLTALISWLLACIIVKTVVPGIVLMIIAMVGGKAATAIFGAITLG
                     CVAATAVAAMQSSRLTALLIQDLKEGKASHMDGRLNISHEEDDGLGLDKFHDRKH"
BASE COUNT          494 a          748 c          682 g          404 t
ORIGIN      
        1 taacttggcc gagggcggtg gcaagcaaag ccgcggtccc gaggcagagt actacgcagg
       61 ccagcgttcg ccgccagccg cgccggtccc ccctgccatt gggtgccctc atatctaaat
      121 catagactga gcgtgaccac gacatgatgc aaaacgttgg agtgaggccc tcgaatcacg
      181 ggttgaccga gcatggcctg cgtaattggg acgtcgccta ttggaacctg ggcacggcga
      241 agctggtcga agcggctatt cagcgccacg aaggcgagct ggccaacacg ggagcgcttg
      301 tcgtgcgcac ggggcatttc acgggacgct cgcccaagga caaatacatc gtccgcgacg
      361 ccggaacgga gaacaccgtc gcctggggca gcgtgaatca gcccatgagc ccggagaagt
      421 tcaccggcct gtacgacaag ctgctcagct tctggcaggg acgcacgctg ttcgtccagg
      481 actgcctggc gggcgccgat ccgaaacacc agattcgcgt ccgcgtcatc accgaaaccg
      541 cgtggcactc gctcttcggc cggcaactgc tgatccgcac cgcaatcgaa gaattgcagg
      601 atcaggttcc cgatttcacg attatgttcg ccccgacgtt tcaggcgcga cccgaatccg
      661 acggcacgaa ttcgggaacc gtcatcgcga tcaatttcac ggagcgattg gtcctgatcg
      721 cgggcacgat gtatgcgggc gaactgaaga agagcgtctt cacgattctc aactacttgc
      781 tgcccggaaa cgattcgctg cccatgcact gttcggccaa catcggcccc aaaggcgacg
      841 tggctctttt cttcggactc tccggcacgg gcaaaaccac actctccgcc gacgcttccc
      901 gcggcctgat cggcgacgac gagcacggct ggtccgacga cggcgtcttc aatttcgaag
      961 gcggctgcta tgccaagtgc atccacctca gccagaaaca cgagccggag atctggaatg
     1021 cgatccgctt cggcgccgtg ctcgagaacg tgaagctcga cgtgaatcgc cgcaactgcg
     1081 attacgactc cgacgaattc acagagaata cccgagcggc ctatcgcatc gagttcattg
     1141 caaacgcggt gaccccgagc gtgggaggcc accccaagaa catcctgttc ctagccgccg
     1201 atgccttcgg cgtgctgccg ccgatttcgc gcctgacgcc cgaacaggcg atgtaccact
     1261 tcctgagcgg atacacggca aagctagccg gaaccgagcg cggcctcggc aaggagcctt
     1321 cagcgacctt cagcgcctgt tttggcgaag tgttcctgcc tcgcccggcg catgtctatg
     1381 cggaattgct gggaaagaag atgcgcgaac acaactcccg ctgttggctg gtgaataccg
     1441 gctgggtcgg cggcgcattc ggaaccggct cgcgcatgag cctgccctac acacgggcca
     1501 tcgtgaacgc ggccatcgcg ggccaactcg acgacgtgtc caccgtcacg cacccggtgt
     1561 tcggcgtcgc catgcccact tcgtgccctg gcgtcccgag cgaactgctg gatcctcgca
     1621 gccagtgggc cgacaaggcc gcctacgatg ccgccgcacg agatctcgcg ggccggttcc
     1681 agaagaactt cgccaaattc ggaacaatcg attccgcaat cgccaacgcc gggccaaagg
     1741 ccggctagcg gccgttccgc ttcgaaacag aactcgccga acaactcttc atctccttca
     1801 ctctaaaggg ccgctaggcc ccagtcgatc tatcgagaaa gaagagaggc gagtgcatgt
     1861 cctatctcga gtacgtaacc gtcgatcacg aatctgacga tccgatgccc gaactggcga
     1921 ggaccaatgg gttcagcgaa tccgatctgg cactgaaccg cgccggcaag atcagcgatc
     1981 ctcaacggag caagctcttc ggacgcgcgt tgagaccggt gaagtatccg ctgactgcgc
     2041 tgatcagttg gctactggcc tgcatcatcg tcaagaccgt ggtgcccggc atcgtgctga
     2101 tgatcattgc aatggtggga ggcaaagccg ccaccgcgat cttcggagcc atcaccctgg
     2161 gttgcgtggc cgcaaccgct gtcgccgcga tgcagagcag ccgcctgact gccctgctga
     2221 ttcaggattt gaaggaaggc aaagccagcc acatggacgg gcggctcaac atcagccacg
     2281 aagaagacga tgggctcggc ctcgacaaat ttcacgaccg caagcatc
//
LOCUS       DASUGB010000161         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_436013, whole genome shotgun sequence.
ACCESSION   DASUGB010000161 DASUGB010000000
VERSION     DASUGB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436013"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFV87_03165"
     CDS             complement(<1..621)
                     /locus_tag="VFV87_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912107.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5102780.1"
                     /translation="MSKKRFRPRSHGGRTGGGGGYGGGGGYYQGSNGQGGGGGRSRRR
                     RYYQDRERPPFGGEAAPAPVGEDGNPLPEGAEGTEFQNPPVTPEQVLEPGYGLLEMHP
                     NGYGFLRSPDTTYTRERSDPFVPGTMIEKYRLRQGVMIKGTVQPARRQQGPRLRDITD
                     VDGMPPDEYPNVKTFDAKTPINPDKWFRLETGPTPLTTRVMDLLTPL"
     gene            complement(1638..2792)
                     /locus_tag="VFV87_03170"
     CDS             complement(1638..2792)
                     /locus_tag="VFV87_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102781.1"
                     /translation="MRIAVVEALGRIGGNRAVEILAPHMNEADVSVRQSVANVLGRIG
                     GNRAVEILEASATDEDEAVRRIALAGLRANQGLVRDPTTRARFAVEKKAWDKVAQMGQ
                     AAIGPLQDYFDSGGDGPGAVEALTNIGGPEVPAMLVSLLTRRDFYWEARTALIRLRER
                     AVDALFAGLDSNNDQTRSYSGQILGEIGDRRTLGRLIAAMADEEVLRRIDPDWKTSEE
                     ARQAIPTLAGLAAVKGGRNALEAGWRLVYIGRATQTAEPFIAALRCKEFDFNSECSPR
                     GIAIGDMRGLRDAQAFGPLLDFIDDPAYAATILGFLTEILKCSAAEIASAILQKAASL
                     PDVLHSVHVGDTYIPDTSTTGWTRSDYEDRPLDCSSLRKLACDELNRRKT"
     gene            2691..3470
                     /locus_tag="VFV87_03175"
     CDS             2691..3470
                     /locus_tag="VFV87_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102782.1"
                     /translation="MAHRNVRFVHVRGEDLHGPITADSPECFHYRYAHSIVFVVYAAC
                     ERCNRPRPADAAEFVNDGRSHVRVRFANPRRKDFGRQWPADFSECLRHGLSDACVVVD
                     GARGENRSSLIAEPSKRPSGAAAEGRIRQLNRFGEQLEGLGAIHQPQCGEREFTTPRR
                     PFPSECSLNQQHSGRPSGDGCLPQRLLGAGGQPPVLGQTIENGFRNLLIDDLRKKRGP
                     IPRRQSERKLFPRTLAFPPHLAEPGYGHLADQRIGIAQGGY"
BASE COUNT          716 a         1285 c         1292 g          776 t
ORIGIN      
        1 gagcggcgtg agcaggtcca tcacgcgcgt ggtcagcggc gttgggccgg tttccaggcg
       61 gaaccatttg tcggggttga tcggcgtttt ggcgtcgaag gtcttgacgt tcggatattc
      121 gtccggcggc atgccatcga cgtcggtgat gtcccgcagc cgtgggcctt gctggcggcg
      181 ggccggctgg acggtgcctt tgatcatgac cccctgacgc aggcggtatt tttcgatcat
      241 cgtgccgggc acgaaggggt cgctgcgctc gcgggtgtag gtcgtgtccg ggctgcggag
      301 aaagccgtag ccgttggggt gcatctccag gagtccgtag ccgggctcga gcacctgctc
      361 gggcgtgacc ggcgggttct ggaattcggt cccttcggcc ccttccggca gcggattgcc
      421 gtcttcgccg accggcgccg gagcggcttc accgccgaag ggcgggcgct cgcgatcctg
      481 gtagtagcgg cgacgccgcg aacggccccc gccgcccccc tgaccgttgg atccctgata
      541 gtagccgccg ccgccgccgt agccacctcc accacccgtg cgaccgccat gtgaccgggg
      601 acgaaacctc ttcttcgaca tgaatcaccc agaaagagag cgcgacacgc ggatagatgg
      661 tcgcgcgacc agagaaagaa acgaattcct cgttatggcg gaacggcgtg cgacggcatg
      721 tcgctcccgt gcagccggaa agaccggcac gcgacggttt ggacgcgatg gcgttccgcg
      781 tgagttcaga tcggactaag cccggcgctt cgctgcgcag tgcttctctg cacttcgctg
      841 caccatgctc agcctgccaa tatggttatg cttctgcgaa cgacgaccgc cactggctgt
      901 aatccccgag gccagcgacc gcgggcgatg gcaacctggc atttccgtcg aactgcaagc
      961 ggtggccgca gcgtgcgatc gtatcgagcg tgcgatgggt atcgcggctg ggctagttgc
     1021 ttctccgcct cctgctgcaa actacattcc tggcttccct tccgaattgg cgaacaggct
     1081 tcctggttcg gattcggatc ggaaacaagg ctggaagcac gctgcttaaa atcaagcgac
     1141 caagcagggc acatccaggg tcagagaagc gtctgcaggc cgggcagggt tggttgttgc
     1201 agccgcggct tgccaccacc cgccactggt gggcgggcga aacgcttaca ggttccacag
     1261 aaacagaagc tgtactactg cgaatcttat tgaactctcg gcaggtgtca agctctggaa
     1321 tgagcctggc gcgcggaatt cgggaaataa atgcggccgg aaagccttgg cgcggacagg
     1381 gttgcggcgg gagcggccac tgccgtctgt cgtgccagag cgagcagatt gccggccgcc
     1441 ggcctttccg gaccggctcg ccacgattgt cccttcaatc gcaagatttg tttcaacgga
     1501 caaaggagtc aggatgaagc ctgaacccct tttggcgctc ctttgcgcct tttgccggcc
     1561 ccaccgattg cgatggcgga aacaccgggc cgaaatcgtc cattgcgagc aacctgggcc
     1621 atcccgcggc tcacctttca tgttttccgg cgattcaact cgtcacaggc cagttttctc
     1681 agggacgagc agtccagggg acgatcctcg tagtccgacc tcgtccatcc ggtggtgctg
     1741 gtgtcgggaa tgtaggtgtc gcccacgtga actgagtgca gtacgtcggg gagcgaagca
     1801 gctttttgca gaattgcaga ggcgatttcc gccgccgagc acttcaatat ttcggtgaga
     1861 aatccaagaa tcgtagcggc atacgccggg tcgtcgatga aatcgagcag aggcccgaac
     1921 gcttgggcgt cgcggagtcc cctcatatcg ccaatcgcga tcccgcgggg agaacactcg
     1981 ctgttaaagt cgaactcttt acaccgcagt gcggcaatga atggttccgc ggtctgtgtg
     2041 gctctgccga tatacacgag tcgccaaccg gcctccagcg cattacgtcc ccccttcacc
     2101 gccgccagac ctgctagtgt tggaatggcc tggcgggcct cctccgatgt cttccagtcc
     2161 ggatcgatcc tgcgcaacac ctcctcgtcg gccatcgcgg cgatcagccg gcccagcgtg
     2221 cggcggtcgc cgatctctcc caagatttgg ccggaatagg agcgagtctg gtcgttgttg
     2281 ctgtcgaggc cagcgaagag agcatcgaca gcacgctctc gcaaccggat cagtgcagtc
     2341 cgggcctccc agtagaagtc ccttcttgtt agcagcgaca ccagcatggc gggcacttcg
     2401 ggaccgccga tgttcgtcag ggcctcgacg gcgccggggc cgtcccctcc agaatcaaaa
     2461 tagtcctgca gcggcccgat cgccgcttgc cccatctgcg cgaccttatc ccaggccttc
     2521 ttttcaacgg cgaatctggc gcgggtcgtg gggtcgcgta ccagaccttg attcgcccgc
     2581 agacccgcaa gagcaatccg cctcacggct tcgtcctcat ccgtggcact cgcttcgagg
     2641 atctcgaccg cgcggttgcc gccgatacgc cccagcacgt tcgcaacgga ttggcgcacc
     2701 gaaacgtccg cttcgttcat gtgcggggcg aggatctcca cggcccgatt accgccgatt
     2761 cgcccgagtg cttccactac cgctatgcgc acagcatcgt gttcgtcgtt tacgcggctt
     2821 gcgagcgctg caatcgcccg cggcccgccg acgcggccga gttcgtgaac gacggtcgtt
     2881 cgcacgttcg tgtccggttc gctaatccgc gccgcaagga cttcggccgc cagtggcccg
     2941 ccgatttctc cgagtgcctt cgccacggct tgtcggacgc ctgcgtcgtc gtcgacggcg
     3001 cgcggggcga gaatcgcagc agcctgatcg ccgagccgag caagcgcccc agcggcgcgg
     3061 cagcggaggg acggattcgc cagttgaatc gcttcggcga gcagctcgag gggctcgggg
     3121 ccatccatca gccccagtgc ggagaacgcg agttcacgac accacgacgg ccgttccctt
     3181 cggaatgcag ccttaaccaa cagcattccg ggcggcccag cggcgacggc tgcctccctc
     3241 aacgcctgct cggcgccggc gggcaaccac ctgtcctcgg tcaaaccatc gagaacggat
     3301 tccgcaacct gctcatcgac gatctgcgca aaaaacgcgg accaatcccc cgccgccaga
     3361 gcgaacgaaa gttgttccct cggacgttgg cctttccacc ccatctcgcg gagcccggct
     3421 acggccatct tgcggatcag cgaatcggaa tcgcgcaagg cggctactag cggcgcaaca
     3481 acttgcgggt cattccactg ccacaatcct tcgatcgccc tctgccggac tctcgcgaca
     3541 gggtcgcgca gcgcaggggc gagcattgga atcacgcggg gattgttcga ctgggcgagt
     3601 ttcgaggcgg cctcttcccg cacgattggg tctttggacc tcagcctgta cttccaccac
     3661 caaagcatgt cgtacccacc gtcaagggcc atgaccgcga gctccgctca actgagcacc
     3721 gtcagtggtc agcacgatta aacgcgacat tcgggcgagt gtcaatttgc tgcgctcgct
     3781 tacaagcctc ccggtggtat tccttggggg agcgggagtc aggcttcatt gtttggtctt
     3841 tcgcgggcgg ccacagcctt cgctgtgggc tgagggcccg cgccgctctg cggtggaatg
     3901 gaccagaggt gtccattccg gaggggcggg tggaaaaaag ttagagcgag gcgtgggggc
     3961 ggaaggtgtt ggtggacagg agtttatgcg cgggcgggtt gccgtaagtt ggggtggggt
     4021 gagaaaatcg gcggaatttg cttggagcgc agggaggcga ggtgtaaac
//
LOCUS       DASUGB010000162         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1548282, whole genome shotgun sequence.
ACCESSION   DASUGB010000162 DASUGB010000000
VERSION     DASUGB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548282"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFV87_03180"
     CDS             complement(<1..789)
                     /locus_tag="VFV87_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5102783.1"
                     /translation="MASVFVVPRRSVLKIGIGLAGLPLLGGCSSQPAGNQPVSSKGSK
                     GVVGVSVLTLTNPFFKVIGDNITAELKKAGYDTIVVSGDKDIATQQRQVRDFVVRKAA
                     AIVLCPCDSRAIGPVIVEANQAGIPVFTCDIASLADTGKVVSHIATDNYGGGKLAAEA
                     MIEALGPAGGDIVILDFRSAESCLERVRGFKEVLGAHNASRETGKINIVAELPGEGAQ
                     DRGYAAAEDALTAHPDLAGIFAINDPSALGARQALEKAGKADQVK"
     gene            1102..2046
                     /locus_tag="VFV87_03185"
     CDS             1102..2046
                     /locus_tag="VFV87_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX5102784.1"
                     /translation="MSLVSLRHRAEYLALRVAMSLILAVSMRTCHALCRTLAWLMCDV
                     IRLRHKLIDENLQVAFPSATASERTRLARAMWEHLLVMSCEIAHAPRKIHETNWRKFV
                     AVHRKRELIRAFLEPRPKVVVTAHFGNFEIGGYITGFWGFPTFTVARPLDNPYLDRLV
                     NRFRESMGQVIIPSHGSAEMADGLLSTGGTLVLVGDQHAGSKGVWVDFFGRPASCHKA
                     LALFTLLNKAPMLVTYVRRTGKPLHFEVGLADAYDPAEPGGDDLAGVTELTQWYNDVL
                     EEEIRRSPEQYWWLHNRWREAPAGRRRKPKRRHARRAA"
     gene            2190..2528
                     /locus_tag="VFV87_03190"
     CDS             2190..2528
                     /locus_tag="VFV87_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5102785.1"
                     /translation="MSRWIRLAAAADCPPGAAREFVAGDRIVALFNVGGTFYALDGVC
                     PHQGGPLGKGQLSGCIVTCPWHGFQFDVTSGQHQTSKSLVHPRFEVKVEGPDVFLDAD
                     YPAETRNLKP"
     gene            2554..3501
                     /locus_tag="VFV87_03195"
     CDS             2554..3501
                     /locus_tag="VFV87_03195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5102786.1"
                     /translation="MIRYRPFQNWDPPALAEIWRTQPPLPGRVQAVTPPLLEKLVFAK
                     PWFDHNGLIVAVDGARPVGFAHAGFGASTDQCNIDMHEGTICQLMVSPHEQRGIIAVE
                     LLSAAEDYLRRRGAQHIHAGCQFPMNPFYLGLYGGSQAPGVLDSDTQFSELVAGAGFR
                     PDRRRLLWRRPLAGFRSPVDRQWMQVRRRFVAAGPREVLPDNWWDACVWTHFDWARYD
                     LNLPGGGESVISATLWDIEPLARSWGRQTAGLVRLDDTPEARDEGLTGFLLGEILRLC
                     QAEGYAQFEAQTSDSDAPLAELLVRLGFEQYDRAALWSK"
     gene            complement(3526..3768)
                     /locus_tag="VFV87_03200"
     CDS             complement(3526..3768)
                     /locus_tag="VFV87_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102787.1"
                     /translation="MTKTTSPFRLPATLGLLGLAVAALGSTGCQGMYAGQTLPSPYYM
                     QDDIQYFPPGAEFKLAREAAAQKAYKADLQAAGAIP"
BASE COUNT          688 a         1338 c         1241 g          741 t
ORIGIN      
        1 cttcacctgg tcggccttgc ccgccttttc cagggcctgg cgcgcgccga gggccgacgg
       61 gtcgttgatg gcaaaaattc ccgccagatc gggatgcgcg gtcagcgcgt cctcggctgc
      121 tgcataaccg cggtcctgcg ccccttcgcc gggaagctcg gccacgatat tgattttgcc
      181 cgtttcgcgg ctcgcattgt gggcgcccag cacctccttg aagccgcgga cccgctccag
      241 gcacgattcg gccgagcgga aatcgaggat gacgatgtcg ccgcccgcag ggccgagggc
      301 ctcgatcatc gcctcggcgg ccagcttgcc cccgccgtag ttgtcggtgg cgatatgcga
      361 gacgaccttg cctgtatcgg ccaggctggc gatgtcgcaa gtgaacaccg gaattcccgc
      421 ctggttcgcc tcgacgatca ccggcccaat cgcccggctg tcgcaggggc acagaacaat
      481 ggccgccgcc ttgcgaacga cgaagtcgcg cacttgccgc tgctgcgtgg cgatatcctt
      541 gtcgccgctg acgacgatcg tgtcgtaacc cgccttcttg agctcggcgg tgatattgtc
      601 gccgatcacc ttgaagaacg ggttggtcag cgtgaggacc gagacgccga ccactccctt
      661 gcttcccttg ctagagacag gctgattgcc cgcaggctgg gaactgcaac cgcccagcag
      721 cggcaagccg gccagtccga tgccgatctt aagcacgctc ctccgcggca caacgaatac
      781 cgacgccatg gaaacctcct cgcgtgaatg gcaagccgcg gactgtggag cgacgaacaa
      841 gctacaccac aaagacactt tagccacgga agaaacacgg ggccacccgg atgggaaaca
      901 tcggcaggac ctgatcatcc aaattctctt tcattcgtgt ttcatctgtg ttccatccgt
      961 ggctacccga ctttcccagt attcgctcgt cttcaacatg tttcccttcg tggtacgaag
     1021 ggaaagtgct cttggtgacc catcgcggcc cggtcaaaat gggcagtgtt tcgcagggag
     1081 cgctctcgct gaggacaggg aatgtcgctg gtttcgctcc gccatcgggc ggaatatctg
     1141 gctcttcgcg tcgccatgtc gctgatcctg gcggtcagca tgcggacgtg ccacgcgctc
     1201 tgtcgcacgc ttgcctggct gatgtgcgac gtaatccgtc tgcggcacaa gctgatcgac
     1261 gagaacctgc aggtcgcctt tccgagcgca acggccagcg agcgcacccg tctggcccgc
     1321 gccatgtggg agcacctgct ggtcatgtcg tgcgagatcg cccacgcccc gcggaaaatc
     1381 cacgagacca actggcggaa gttcgtcgcg gtacaccgga agcgggaact gatccgggcg
     1441 tttctcgagc cgaggcctaa ggtagtcgtc acggcccatt tcggcaactt cgagatcggc
     1501 ggctacatca ccggcttctg gggctttccg acgttcaccg tcgcccggcc gctggacaat
     1561 ccgtatctgg atcgcctggt gaatcgcttc cgcgagtcga tggggcaggt aatcatcccc
     1621 tcgcacggca gtgcggaaat ggccgacggc ctgctaagca cgggagggac gctcgtgctg
     1681 gtgggggatc agcacgcggg gagcaaaggg gtgtgggtcg attttttcgg ccgccccgcc
     1741 tcgtgccaca aggccctggc cctgttcacg ctgctcaata aggccccgat gctcgtgacg
     1801 tatgtccgcc gcaccggcaa gccgctgcac ttcgaggtag gtctggccga cgcatatgat
     1861 ccggccgagc cggggggcga cgacctggca ggggtcaccg agctgacgca gtggtacaac
     1921 gacgtgctgg aggaggaaat ccgccgctcg cccgagcagt actggtggct gcacaaccgc
     1981 tggcgggaag ctcccgccgg ccgccgccgc aagcccaaac gccgccacgc gcgccgcgcg
     2041 gcttgagccg ccgttaaagc ctccgggcga ttcgcgggtc gtcgcaatcg cgccaccgcg
     2101 gccagctcga tccgctacta tagaagggtg cgcctcgact gtcgttgttt cctgcagttt
     2161 gccgccgaaa tctgccacga gcctttgccg tgagtcgctg gattcgcctt gccgccgccg
     2221 ccgattgccc gccgggcgcc gcccgcgagt tcgtcgccgg agaccgcatc gtcgctctgt
     2281 tcaacgtcgg cggaacattc tatgcactcg acggcgtctg cccgcaccag ggtggaccac
     2341 tcggcaaggg ccagctcagc ggctgcatcg tcacctgccc ctggcacggc tttcaattcg
     2401 acgtgacctc cggccagcac caaacgagca aatcgctcgt ccatccacgt ttcgaggtga
     2461 aggtggaagg cccagatgtc ttcctcgacg cagactaccc cgctgaaact cgaaatctga
     2521 aaccctgaac cctgaacact cgccttccgc cccttgatcc gctatcgccc tttccaaaac
     2581 tgggatccgc ccgcgctggc cgagatttgg cgcacgcagc ccccgctccc cggccgcgtg
     2641 caagccgtaa ctccaccgct gctcgaaaaa cttgtcttcg ccaagccctg gttcgaccac
     2701 aacggcctga tcgtggccgt tgacggcgct cgccccgttg gctttgccca cgcggggttc
     2761 ggtgcgagca cggaccaatg caacatcgac atgcacgaag gcacgatctg ccagctcatg
     2821 gtctcgccgc acgagcagcg gggaatcatc gccgtcgaac tgctctcggc cgccgaagat
     2881 tacctccgcc gccgcggggc ccagcatatc cacgccggct gccagtttcc aatgaatccg
     2941 ttttatttgg ggctgtatgg cggcagccag gctcccggcg tgctcgattc cgatacgcag
     3001 ttcagcgagc tggtggccgg cgccggtttt cgccccgacc gccgccgctt gctgtggcgg
     3061 cggcccctgg ctggctttcg ctcgcccgtc gatcgccagt ggatgcaggt ccgccgccgc
     3121 tttgtcgctg ccgggccgcg ggaagtgctg cccgacaatt ggtgggacgc ctgcgtctgg
     3181 acccatttcg attgggcccg ctacgacctg aacctgcccg gcggcggcga gagcgtcatt
     3241 tcggccacgc tctgggacat cgagcccctg gcccgcagtt ggggccgtca gacggccggg
     3301 ttggtgcgcc tcgatgacac tcctgaagcc cgcgacgaag gacttaccgg attcctgctc
     3361 ggcgaaatcc tgcggctgtg ccaggcggaa ggctatgcgc aattcgaggc ccaaacgagc
     3421 gacagcgacg cacctctagc cgaattgctg gtgcgcttgg gcttcgagca gtacgatcgg
     3481 gccgcgctct ggagcaaatg agctgctccc attcggtccc ggtcgctatg gaatcgcccc
     3541 agcggcttgg aggtcggcct tatacgcctt ttgagcggcc gcctcgcggg ccagcttgaa
     3601 ttccgcgccc ggcgggaagt actggatgtc gtcctgcatg taatacggac tgggaagcgt
     3661 ctggccggca tacatcccct ggcagcccgt ggagcccagg gcggcgacgg ccagtcccag
     3721 gagacccaac gtcgcgggca accggaaggg cgaagtagtc ttggtcacga gcgtgttctc
     3781 cgattggccg cacgttcggc aagggcgtca ttgtcgctac aaacgtcacg gtcaattatc
     3841 ggcgctggga cgcgagaact ttggccaagc agaagaaatt tgccggcttc gcaatagcgg
     3901 gcggacatca tgaaggtccc cgccgctagc aggtcacact agcaatcgcc ggcagaccac
     3961 gttagtttgc gcactgcctg agtcgtttcc caagctggac gtcgcgga
//
LOCUS       DASUGB010000163         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1130402, whole genome shotgun sequence.
ACCESSION   DASUGB010000163 DASUGB010000000
VERSION     DASUGB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130402"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV87_03205"
     CDS             <1..225
                     /locus_tag="VFV87_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5102788.1"
                     /translation="ANSSKWLNEEKAKFHKFAWQDGFGAFTVSQSQIERIAGYIRNQK
                     NRHKKSDFKTEFLELLVKNEVEYDVRYLWT"
     gene            216..380
                     /locus_tag="VFV87_03210"
     CDS             216..380
                     /locus_tag="VFV87_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102789.1"
                     /translation="MDVERFREQRLPTAQRCNRGGPNHWEIWLRDGEGYTIVLASPDG
                     TADGNWKPGA"
     gene            complement(395..1399)
                     /locus_tag="VFV87_03215"
     CDS             complement(395..1399)
                     /locus_tag="VFV87_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5102790.1"
                     /translation="MPTSTALEGNSTAQFLSNLYAPVAAEMEEVERILKTELRNPHPF
                     VDELVRYGCLLGGKRMRPALLLLTAKAIGPVRRDHLVLAAVVEMIHTATLVHDDVLDE
                     ADMRRHLATVNSRWDNEASVLLGDYLFTHAFYLASTLDSVLGCRLIGRATNIVCEGEL
                     RQKGSRGNFELGEQEYLSIIEGKTAELTAVSCRLGAHFAGASEKLAGEMEGFGRDLGI
                     AFQIADDLLDVLGEERATGKSLGTDFEKQKPTLPVIRALELATPTDREELLELLSGDE
                     RRPDLVTPYLERYEALDYARERAVSFATGARRRLDALPKSAAKDILSLMTKFVVTRAS
                     "
     gene            complement(1502..1969)
                     /locus_tag="VFV87_03220"
     CDS             complement(1502..1969)
                     /locus_tag="VFV87_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102791.1"
                     /translation="MDEANQLALITIAVAVGVVIGLLLGAVILRAAVSLSNQIAPATS
                     PAGKLEVPSFGKAFAIVFFTNLAICLVSFGAGLVLGTGGGAIGLSRTAVFSLAQVISL
                     PLSFLVMSFMLSNQLKTTMARGMGISLCYLAVVLAMVVVGVVVAMVLAPVLFR"
     gene            complement(2075..>2393)
                     /gene="moaC"
                     /locus_tag="VFV87_03225"
     CDS             complement(2075..>2393)
                     /gene="moaC"
                     /locus_tag="VFV87_03225"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5102792.1"
                     /translation="EVARLAGIMAAKKTAELIPLCHPLALDGVTVGFTFPDERTVAIE
                     AEVKITARTGVEMEALTAVSVAALTVYDMCKAIDRGMVIADIRLEEKSGGRSGHFRRD
                     GRP"
BASE COUNT          502 a          728 c          717 g          446 t
ORIGIN      
        1 gccaactcgt cgaaatggct gaatgaagag aaagcgaagt tccacaaatt tgcctggcaa
       61 gatggatttg gcgcgttcac ggtgagccag tcccagatcg agcggatcgc gggttatatt
      121 cgcaatcaga agaaccggca caagaagtct gatttcaaaa cggaattcct ggaactgctc
      181 gtgaagaatg aggtggagta cgacgttcgg tatctgtgga cgtagagcga tttcgcgagc
      241 aacgcctccc caccgcgcag cggtgcaacc gcggcggccc gaaccactgg gaaatctggc
      301 tccgcgacgg cgaaggctac accatcgtcc tcgccagccc ggacggcacc gcggatggaa
      361 attggaagcc gggggcgtaa gctcgcggca ggcgctagct cgcccgggtg acgacgaact
      421 tcgtcatgag agagagaatg tccttcgcgg cgctcttggg gagcgcgtcg aggcggcggc
      481 gggcaccggt ggcgaaggag acggctcgct cgcgggcata gtcgagagct tcatagcgct
      541 ccagataagg ggttacaagg tcgggccggc gctcgtcgcc gctgagcagt tccagcagct
      601 cttcccggtc cgtgggggtc gccaattcca gggcccgaat gaccggcagc gtcggctttt
      661 gcttttcaaa atcagtcccc agcgacttgc ccgtcgcccg ttcttccccg agcacgtcga
      721 gcaggtcgtc ggcgatttga aaggcgatcc ccaggtcgcg gccaaagccc tccatctcgc
      781 cggcaagctt ttcggatgcg cccgcgaagt gcgcccccag ccggcaactg acggcggtca
      841 gttcggccgt cttaccctcg atgatgctca ggtactcctg ctcccccagc tcgaagttgc
      901 cgcggcttcc cttctgccgc agttcccctt cgcagacgat gttggtggcc cggccaatca
      961 gccggcagcc gaggacggag tcgagagtgc tggcgagata gaaggcgtgt gtgaacaggt
     1021 agtcgccgag gagaacgctg gcttcgttgt cccagcggga attgacggtg gccagatgcc
     1081 gccgcatgtc ggcttcgtcg agcacgtcgt catgcacgag cgtggcggtg tgaatcatct
     1141 cgacgacggc ggccagcacc aggtgatcgc gccggaccgg cccgatggcc ttcgccgtca
     1201 gcaggaggag ggccggccgc atccgcttcc cgccgagcaa gcagccgtag cggacgagct
     1261 cgtccacgaa cggatggggg tttcgtagct cggttttcag aatccgctcc acctcctcca
     1321 tctccgccgc caccggggcg tagaggttgg aaaggaattg agcagtggaa ttgccttcca
     1381 gggcagttga agtaggcata acgggtgaat ccttgctcgc gcggaggttt ccttcaaaag
     1441 agaggggaca ctgctgaacg agtcccagga ctattttcag ataatctcac ggcgcagatt
     1501 cctatcggaa taaaactggc gcgaggacca tcgccacaac gactcccacg acgaccattg
     1561 cgagaactac cgccagataa caaagcgaga ttcccatgcc tcgcgccata gttgtcttca
     1621 gttgattgga aagcatgaat gacatgacca agaaactgag tgggagcgag atcacttggg
     1681 ccagcgaaaa aactgccgtt ctggacagac cgatggctcc gcctcccgtg cccaatacca
     1741 ggcccgcccc aaaactcacg aggcaaatcg caagattcgt gaagaataca atcgcgaatg
     1801 cctttccgaa ggaaggcact tccaatttgc ctgcagggct ggtggctggg gcaatttggt
     1861 tggacaacga tactgccgct cgaagaatga ctgcgccaag caacaatcca atcacgaccc
     1921 ccaccgctac ggcgatggtg atcaatgcca attggttcgc ctcgtccacg gagtgggagc
     1981 ctctcttctg ggcgcatcgg ttgcctgcgt ggcacaaatg cgatcagcgc ggcggattgt
     2041 tctctgctgt ggatcactca gccaaaaccg caattcaggg ccggccatca cgcctaaaat
     2101 gaccgcttcg accccctgac ttttcctcca gccgaatatc ggcgatgacc atcccgcggt
     2161 caatggcctt gcacatgtcg taaacagtga gagcggcgac acttacggcc gtcagggctt
     2221 ccatctccac tcccgtcctg gccgtaattt tgacctcggc ctcgattgcc accgtccgtt
     2281 catccggaaa agtgaacccg accgtcaccc cgtccagcgc gaggggatgg cagagcggaa
     2341 tgagctccgc cgtcttcttt gccgccatga ttcccgccag ccgggctact tcg
//
LOCUS       DASUGB010000164         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_992412, whole genome shotgun sequence.
ACCESSION   DASUGB010000164 DASUGB010000000
VERSION     DASUGB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992412"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VFV87_03230"
     CDS             complement(<1..1353)
                     /locus_tag="VFV87_03230"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5102793.1"
                     /translation="MTNDQAAAEKLIPPDVIPPGDLPPLRERDLPPPISLARMIGPSI
                     ILAGLALGSGEFVLWPYITFKSQFVFFWACLLAVTMQYFLNLEIMRWTLATGETAMTG
                     IIRLNRHMAWAFLLLNIIPWMLPAWATGAADLIGWLIAEPTFKPDGNVIPGPYNVWIA
                     LAGLILCGVILTAGPIVYETVEKVQLFLVSLIMLTVICLAVWLLKDRPDAIAAQAVST
                     VTLGAPQFLPPLDDDLTPIALLGALAFAGAGGTMNLCQSNYIRDKGYGMGCHIGRITS
                     PITGQEEAISEVGYHFPHTVENLRRWAAWWRGACTEHFFSFLMTCLVCLVLLTLISYV
                     TFYDAAGQRLHDPGTIKGMRFIWEESLHVGELIGPAARLAFLIMGVAILFTTEFGVLD
                     AASRVSTDIVKVAWLRENQTWSEGRLYYAFLWGEIALACAILLLNKYTGLDLGALALF
                     K"
     gene            1604..1861
                     /locus_tag="VFV87_03235"
     CDS             1604..1861
                     /locus_tag="VFV87_03235"
                     /inference="COORDINATES: protein motif:HMM:NF036547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5102794.1"
                     /translation="MSPTLELGTQSIAVFAAASHGSPGPFASAIDCIGSLAERVNLVE
                     RQIIQACLARHNFRRKDTAAELGISRVTLYNKMKKFGLLEV"
     gene            1888..2577
                     /locus_tag="VFV87_03240"
     CDS             1888..2577
                     /locus_tag="VFV87_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5102795.1"
                     /translation="MPSIADQAIPGGWTERNVVVGHHAFRLILPADPDSLLEHLEDPQ
                     TTSVPHLADPYWAKLWPAAQHLAEALIARPFPPGTRVLELGCGSGLAGLAALAAGLDV
                     TFSDYVPWAVQLALENAARNGYLSARGLAFDWRNPAEQAWPVIIAADVTYDRTNIDPL
                     LETLHRMLAPGGIAWLGDAGRGPVADFVRDASAHGWSTRLLDKSGQERFMPVLGAFQL
                     IVLERAPTANF"
     gene            complement(2587..>3081)
                     /locus_tag="VFV87_03245"
     CDS             complement(2587..>3081)
                     /locus_tag="VFV87_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5102796.1"
                     /translation="LKLAPSLVQCSALGEPVFTPATKEESGHDINISFERMAEIIDAE
                     TAAALKQKSLDIYRCAADHALSRGIIIADTKFEFGWHEGRLILIDEVLTPDSSRFWPA
                     DQYHPGKSQPSFDKQFLREWLETTGWDKNSPPPPLPDDVVERTRAKYIEAYERLTGRT
                     FLLD"
BASE COUNT          575 a         1008 c          963 g          535 t
ORIGIN      
        1 cttgaagagg gccagggcgc cgagatcgag gcccgtgtat ttgttgagca gcaggatggc
       61 gcacgccagg gcgatctcgc cccacaggaa ggcgtagtac agccggcctt cgctccaggt
      121 ctggttttcc cgcagccagg cgaccttgac gatgtcggtc gagacgcggc tggcggcgtc
      181 gagcacgccg aattcggttg tgaagagaat cgccaccccc atgatcaaaa acgccagccg
      241 ggccgccggg ccgatcagct cgcccacgtg cagcgactct tcccaaatga accgcatgcc
      301 cttgatcgtg ccgggatcgt gcagccgctg gcccgcggcg tcgtagaacg tgacgtagct
      361 gatgagcgtc agcagcacca ggcacaccag gcacgtcatc aggaagctga agaagtgctc
      421 ggtacacgcc ccgcgccacc aggcggccca acggcggagg ttctcgaccg tgtgcgggaa
      481 gtgatagccg acctcgctga tcgcctcttc ctggccggtg atcgggctgg tgatgcggcc
      541 gatatggcag cccatgccat agcccttgtc gcggatgtag ttgctctggc agaggttcat
      601 cgtgccgccg gctccggcaa aggccagcgc gccgagcagg gcgattgggg tcagatcgtc
      661 atcgagaggc ggcaggaact gcggcgcgcc gagcgtgacg gtcgagaccg cctgtgcagc
      721 gatggcgtcg ggccggtctt tgaggagcca caccgcaagg cagatgaccg tgagcatgat
      781 cagcgagacg agaaagagct gcaccttctc gaccgtctca tacacaatcg gccccgcggt
      841 caggatcacg ccgcagagaa tcagcccggc cagcgcgatc cagacgttgt acggcccggg
      901 aatcacgttg ccgtcaggct tgaacgtcgg ctcggcgatc agccagccga tgagatcggc
      961 cgcgccggtc gcccaggccg gcagcatcca ggggatgatg ttcagcagca aaaacgccca
     1021 cgccatgtga cggttcaggc ggatgatgcc ggtcatcgcc gtttcgcccg tggccagcgt
     1081 ccagcgcatg atctccaggt tcaggaaata ctgcatcgtg accgccaaga gacacgccca
     1141 gaagaacacg aactggctct taaacgtgat gtacggccag agcacaaact cgccgctgcc
     1201 gagtgcgagg cccgcgagga tgatgctcgg tccgatcatc cgcgcgaggg agatcggcgg
     1261 cggaaggtcc cgctcgcgca gcggcggcag atcaccgggc gggattacgt ccggaggaat
     1321 cagcttttcg gcagcggcct ggtcattggt catgggtcac ttgtcatggg caggtcccga
     1381 ttgtggttcg atcgcggctg aatgccaacc acggcagacc ggccaatcgc cgctatctac
     1441 cccacaaccc gcacattcgg cagcgatctg acccaagttt ccgcccatca aactggccgg
     1501 cgctagtccg ggccggcagg ccaattaacg tggaatcgta gcccaaaccg cggctgattt
     1561 ccgcccgcga ccacgtcccc agaacgcttc gccattgacc gccatgagcc ccaccctgga
     1621 actcggaacg caatccatcg ccgtcttcgc cgccgcctcg cacggttcgc ccgggccctt
     1681 cgcctcggcg attgattgca tcggctcgct ggcagaacgg gtcaacctcg tcgaacggca
     1741 gatcattcag gcctgcctgg cccgccacaa cttccgccgc aaagacaccg ccgccgagct
     1801 cggcatcagc cgcgtcacgc tctacaacaa gatgaaaaaa tttggcctgc tggaggtgtg
     1861 aactgccgcc ggcgtctaca atcgcacatg ccatcgattg cagatcaagc aattcccggc
     1921 gggtggaccg agcgcaatgt cgtcgtcgga caccacgcgt ttcgcctgat tctgcctgct
     1981 gatccggatt cgctgctaga gcatctcgaa gatccgcaaa cgacgagcgt gccgcacctg
     2041 gccgatccgt actgggccaa gctctggccc gccgcacagc acctggccga agcgctcatc
     2101 gcccgcccgt ttccgcccgg aacgcgcgtt ctggagctcg gctgcggcag cggactcgcc
     2161 ggccttgccg cactggcagc cgggctcgac gtaaccttca gcgattacgt tccatgggcc
     2221 gtccaattgg ccctcgaaaa cgcggcccgc aatggatacc tcagcgctcg tggattggca
     2281 tttgactggc gcaacccggc tgagcaggcg tggcccgtga tcatcgctgc cgatgtcacg
     2341 tacgaccgca cgaacatcga cccgctgctc gagacgctcc accgaatgct ggccccaggc
     2401 ggcatcgcgt ggctgggcga tgcggggcgc ggcccggtcg ccgatttcgt ccgggacgct
     2461 agcgctcacg gctggtccac ccgcctgctc gacaagtccg gccaggagcg cttcatgccg
     2521 gtcctcggcg cgtttcaatt gattgtgctg gagcgagccc cgacggcaaa tttctgacgg
     2581 ggaggcctaa tcgagaagga acgtccgtcc cgtcagccgc tcgtaggcct cgatgtattt
     2641 cgcgcgcgtg cgctcgacca catcgtcggg caatggcggc ggcgggctgt tcttgtccca
     2701 gccggtcgtt tccagccatt cgcggaggaa ctgcttgtcg aacgaaggct ggctcttgcc
     2761 gggatgatat tgatcggcgg gccaaaagcg cgagctgtca ggcgtgagaa cttcatcgat
     2821 caggatcaat ctgccctcgt gccagccgaa ttcgaacttc gtgtcggcga tgatgattcc
     2881 acggctgagc gcgtgatcgg cggcgcagcg atagatgtca agactctttt gcttcaacgc
     2941 cgcggccgtt tccgcatcaa tgatttccgc cattcgctcg aaggagatat tgatgtcgtg
     3001 cccggattcc tccttcgtcg ccggcgtaaa gaccggctcg ccgagcgcgg aacactggac
     3061 gaggctcggc gcgagcttca g
//
LOCUS       DASUGB010000165         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1826096, whole genome shotgun sequence.
ACCESSION   DASUGB010000165 DASUGB010000000
VERSION     DASUGB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826096"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..729
                     /locus_tag="VFV87_03250"
     CDS             262..729
                     /locus_tag="VFV87_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102797.1"
                     /translation="MLIAFVVSPWIGLLAALWLYNLYLPSLKQPDDPSIVLACSIVGL
                     AVMAIASWAFMSVAPAILAVPLLCSRLQMRRRRFAVRTRSLRQFVLEYLWHCFAVSVL
                     IAPFVGLACLTLAGDVSSPEKALYFFLGWWSTILVATLIYITPPLRWLLPWLQ"
     gene            736..1845
                     /gene="holA"
                     /locus_tag="VFV87_03255"
     CDS             736..1845
                     /gene="holA"
                     /locus_tag="VFV87_03255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5102798.1"
                     /translation="MSQTLHALEFLASTAKSAPASVCAVFGDEPFLKQLVLKALRRAV
                     IGDEADVPVDTFDCSERLPDWRDVADELATVSLFGGGRPRVVVLERADSFVSANRQRL
                     EDYVSKPSRSSVLILEVDEWPATTRLYKALDQSGLQIDCRPPQKSGRSKGLDETAIVK
                     WIIDWGKSQHAIDLNKDAAQHLLGLTGPSFGLLDQDLAKLALLVPAGTKATPEQVQEI
                     IGGWRSKTIWDLVDAAAGGETAEALTQLDRLLHAGEHPLALVGSLSWSLRRYAAATRI
                     FQQAEREGQKMPLREALVQAGVKDWPMGNLAATEKRLIQLGRHRAGELYRWLLKLDLS
                     LKGSHSQDDRARWALEQLVLRMSKAAAPKPALASR"
     gene            1927..>2558
                     /locus_tag="VFV87_03260"
     CDS             1927..>2558
                     /locus_tag="VFV87_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5102799.1"
                     /translation="MAANLHEIEAVLRLVNRDVWIVTAADGRRRGGLTATWVLPASID
                     SQRPVLLAAIAPNHYTAELIDASGAFAAHLLRPEQTDLAYDFGRDSGRERDKFQGQIV
                     TAAETGSPILADCLAWCDCRVFHRFDAGDRLFFWADVIAARAPSPPAPLPHGAGGTEP
                     RPLVAALGRERGESSQPLCEQEFFRSLSGEQRANLLAGRDADVKIQRPKY"
BASE COUNT          442 a          818 c          801 g          497 t
ORIGIN      
        1 gtggccaaca agcatcggtt caaggtctcc agccgctgcg aggcgctgcg ccgcgccggg
       61 atcgaatcgc aactgcaata ccttggcggt cggcattcac tcgaagtccg cgccctcgac
      121 cggcagcgag cggcggaagt actccaggcg gctcgcgaac gtgaagggag tcatggccgg
      181 cgcaattcac aggttggcgc agttcgcccg aagcggttgt tattctttcg aattcgtccg
      241 acgccatatg ctgcccactc cttgctcatt gcgttcgtcg taagtccctg gattggcctc
      301 ctcgctgctc tatggctcta taacttgtat ctgccctccc tcaagcaacc tgatgacccc
      361 tccatcgtgc tcgcatgtag catcgtcggg ctagctgtca tggccatcgc gagttgggct
      421 ttcatgtcgg tcgcaccagc tattttggcc gttcctttac tatgttcccg cttgcagatg
      481 cgtcgccgac gattcgccgt tcggacccgg agcctgcggc agttcgtgct cgaatacctc
      541 tggcattgct ttgccgtcag cgtcctgatc gccccgttcg tgggtttggc ttgtctcact
      601 ctcgccggag acgtttcgtc acccgaaaaa gcgctgtact tcttcctcgg atggtggtcc
      661 acgattctcg tggcgacgct gatttacatc acgccgccgc tgcgctggct gcttccgtgg
      721 ctacaatagc tgcctgtgtc acagaccttg cacgcacttg aattcctcgc ttcgaccgcg
      781 aaatctgcgc ccgccagcgt ttgcgccgtc ttcggcgacg agccgttcct caagcagctc
      841 gtgctcaagg cgctgcggcg ggcggtgatc ggcgacgagg ccgacgtgcc ggtggatacg
      901 ttcgattgtt ccgagcggct gccggattgg cgggacgtgg ccgatgagct ggcgacggtg
      961 tcgctcttcg gcggcggcag gccgcgcgtc gtggtcctgg agcgggcaga tagcttcgtc
     1021 tcggccaatc gccagcgcct ggaggattac gtctccaaac cgagccgctc gagcgtcctg
     1081 attctggagg tggatgaatg gccggcaacc acgcggctgt acaaggccct cgatcaaagc
     1141 ggtttgcaga tcgactgccg cccgccgcag aaatcgggca ggagcaaggg actggacgaa
     1201 acggcaatcg tgaagtggat catcgactgg ggcaagtcgc agcacgcgat cgacctgaac
     1261 aaggacgctg cccagcacct gctcggcctg accggtccat cgttcggcct gctcgatcag
     1321 gaccttgcca agctggctct gctcgttccc gcggggacaa aagcgacacc cgagcaggtg
     1381 caggagatca tcggcggctg gcggagcaag acgatctggg acctggtcga tgcggctgcc
     1441 ggcggcgaaa cggctgaggc actgacgcag ctcgaccgcc tgctgcacgc cggcgagcat
     1501 ccgctggccc tggtcggctc gctctcctgg tcgctccgcc gctatgccgc ggccacccgc
     1561 atctttcaac aagccgagcg cgagggccag aaaatgccgc tccgcgaagc gctggttcaa
     1621 gccggcgtga aggactggcc gatgggcaac ctcgccgcca cggaaaagcg gctcatccag
     1681 ctcggccgcc accgggccgg tgagctgtac cgctggctgc tcaagctcga tttgtcgctt
     1741 aaaggaagcc attcgcaaga cgaccgcgcc cgctgggccc tggagcaact cgtgctgcgg
     1801 atgtccaaag ccgctgcccc caaacccgcg ctcgcaagtc ggtagccgcg ttggccacaa
     1861 cgcgggaaac tgcgactatt cccgcactct ggcgagtgcg gctactgagc tagaattttg
     1921 ataacgatgg cggcgaacct gcatgagatc gaggctgtcc tgcggctggt gaaccgcgac
     1981 gtgtggatcg ttactgcggc cgatggccgg cggcgaggcg ggctgacggc gacgtgggtc
     2041 ctgccggctt cgatcgacag ccagcggccg gtgctcctgg cggcgattgc cccgaaccac
     2101 tatacggccg aattgatcga tgccagcggc gccttcgcgg cgcacctttt gcgacccgaa
     2161 caaaccgact tggcctatga ctttgggcgc gatagcggcc gcgaacgaga caagtttcag
     2221 ggacaaattg tcacagccgc cgaaaccggc tcgccgatcc tggccgactg tcttgcctgg
     2281 tgtgactgcc gcgtgttcca tcgatttgat gccggcgacc gtctgttttt ctgggcggat
     2341 gtgattgcag cgcgtgcccc ctcacccccg gcccctctcc cgcatggcgc agggggaacg
     2401 gaaccgcgac cactggtcgc ggctttggga agggagaggg gagaatcgag ccaaccactt
     2461 tgcgaacagg agttcttcag aagccttagc ggcgagcaac gagccaacct ccttgccggc
     2521 cgcgatgccg atgtaaagat tcagcgtccc aaatatga
//
LOCUS       DASUGB010000166         4908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1548557, whole genome shotgun sequence.
ACCESSION   DASUGB010000166 DASUGB010000000
VERSION     DASUGB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548557"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1744)
                     /locus_tag="VFV87_03265"
     CDS             complement(347..1744)
                     /locus_tag="VFV87_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5102800.1"
                     /translation="MTAAHLWRLSLIAGFIAALSSAQLAAETAPPKPHIVFLLADDLG
                     YADVGFMGSKEIRTPQLDKLAAGGTILDSFYVQPVCSPTRSALMTGRYPTRTGVYTVV
                     RPHARWGLPLEERTLADALRQAGYHTAVCGKWHLGEFETAYQPTHRGFDEQYGHFFGA
                     LDYFTKLRDGQRDWYRNDQPSADEGYSTHLLATEACRIIRERPKDKPLFLYVPFNAVH
                     APYQVPDSYLGPYGQLAGTRQKYAGMIAAMDEAIGQIAAALDETGIRQDTLIVFSSDN
                     GGPGPGTITDNGPLRAGKGTIYEGGVRVCALANWTGRIPAGTHVTEPIHAVDCFPTLV
                     KLAGGSLEQKLPLDGLDVWSTMTQGAKSPHDAILLCGNPNKAAVRSGDWKLIVTTGGA
                     NADPPAKAAGKAGGKKKAAKKAQAAAQPVELYNLADDLGEKRNLAAEQPERVAQMRAS
                     LDRFLKDAVQPGDQQ"
     gene            complement(1771..3243)
                     /locus_tag="VFV87_03270"
     CDS             complement(1771..3243)
                     /locus_tag="VFV87_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5102801.1"
                     /translation="MTHASRAAPGASLPNIVLIMPDDMGWGDIAAHGNPLIQTPNLDR
                     LYRESVRFTDFHVSPTCAPTRSALMTGRHEFKNGITHTILERERMTLKATTLAQVLKS
                     AGYATGIFGKWHLGDEEPYQPQNRGFDEVFIHGAGGIGQTYPGSGGDAPDNKYFDPAI
                     LHNGTFVKTKGYCTDVFFSQALEWIAGKTKGDAPFFAYITPNAPHGPLISPGAQYDKL
                     YTGKEIDGKQLEEGDVAYYAMITNVDENVGKALAKLKEWGIENDTLVIFLSDNGGTHT
                     RLYSGGYRAGKGSMYSGGTHAPSFWRWPERFQGGVDCPALSAHLDVLPTLAEIVGAQL
                     NGDLAKQVEGRSLLPLLENPKAKWPDRTLVTHVGRWPRGQAAQSKHANSSIRDSRFRL
                     VADRELYDLKSDPGETKNVIDQHPEAVAKLRAAYDQWWDDVQPLLVNEDAIGPKINPF
                     KALYWKQFGGGPDEALRKRMDPAQQNEPPAKAKKAKAKQA"
     gene            complement(3509..>4908)
                     /locus_tag="VFV87_03275"
     CDS             complement(3509..>4908)
                     /locus_tag="VFV87_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5102802.1"
                     /translation="KRGRDDGPFAVDVLTPPDANPWLAQTRLTGLDFFADGDRAAVCS
                     WDGDVWLLSGLRAAAPDSAAASAGAPATLTWQRIASGLFQPLGLKVIEGQIYVTCRDQ
                     IVILRDLNGDGETDFYECFNNDHQVTEHFHEFAMGLQADADGNLYYAKSARHALPALV
                     PHHGTLLRVSRDGLRTDILANGFRAANGVCLNPDGSFVVTDQEGHWNPKNRINWVTLD
                     PSGRPKFYGNMFGYHDVTDSSDSAMEPPLCWITNSFDRSPAELLWVNSKSWGPLDGAL
                     LNLSYGNGKVFVVPHERVGGLMQGGMCELPLPAFPTGIMRGRFHPQDGRLYVCGMFAW
                     AGSATQPGGLYRIRATGRPMHVPLELHCRAGAVTIQLSDPVDPQSVAALDNFAVQTWS
                     LKRTENYGSKHFDERKLRVDAASLASDGKTIQLTIPELHPTWGMEIKISLRATSGQAF
                     ERTIHNTIHSLGEPH"
BASE COUNT          821 a         1597 c         1558 g          932 t
ORIGIN      
        1 aatctcgccc ccatagcagc caatgtccga gaagcccatg tcatcgctca tgatgacgac
       61 gaagttgggc ttctcagcag cataagtttg tccagccagc ggcaaggcgg cggcgaacag
      121 aacggcaagg atggcgtgcg gtttcatgct tctactccag cgggtgcgtg ggcgacctca
      181 gggcgacggc ttcgcgctgt cctggcaaaa aacagaaaga ttcagccgcg ttgcgggctc
      241 gttaagaggt ctggttatag ctgcagggta aaagatgact cccatttttt accgccaatc
      301 tataaccgcc cgttgcagct ctgcggtgtt aaggaacgtg cgcgaattac tgttgatcgc
      361 caggttggac ggcgtctttc aagaagcgat ccagcgaggc tcgcatctgg gcgacgcgct
      421 ccggctgctc cgcggcgaga ttgcgcttct cgcccagatc gtccgccaga ttgtaaagct
      481 cgaccggctg cgctgcagcc tgggcctttt tggccgcttt cttcttcccg cccgccttgc
      541 cggccgcctt cgccggcgga tcggcgttcg cgccgccggt cgtgacgatc aacttccaat
      601 cgcccgagcg gacggcggcc ttgttcggat tcccacacag caggatcgcg tcgtgcggcg
      661 acttggcgcc ttgcgtcatc gtgctccaga cgtcgagccc gtcgagcggc agcttttgct
      721 cgagcgagcc gcccgcgagc ttgaccagcg tggggaagca atcgaccgcg tggatcggct
      781 ccgtgacgtg cgttccggct ggaatgcgac ccgtccagtt ggccagagcg cagacgcgca
      841 cgcccccttc ataaatcgtc cccttgccgg cccgcagcgg cccgttgtcg gtgatcgtgc
      901 ccgggccggg gccgccattg tcgctggaaa acacgatcag cgtgtcctgg cggatccccg
      961 tctcgtccaa cgcggcggca atctgcccga tcgcttcgtc catcgcggcg atcatcccgg
     1021 cgtatttctg ccgcgtgccg gcaagctgcc catacggccc caggtagctg tcgggaacct
     1081 ggtacggggc atggacggca ttgaacggca catacagaaa cagcggcttg tccttcggcc
     1141 gctcgcggat gatgcggcaa gcctccgtgg ccagcagatg cgtcgagtag ccctcatcgg
     1201 cactcggctg gtcgttgcga taccagtcgc gctggccgtc gcggagcttg gtgaagtaat
     1261 cgagggcccc gaagaagtgg ccgtactgct cgtcgaaccc gcggtgcgtt ggctgatagg
     1321 cggtctcgaa ttcgcccaga tgccacttcc cgcaaacggc cgtgtgatag ccggcctggc
     1381 gcagggcgtc ggccagcgtc cgctcttcca gcggcaagcc ccacctggca tgcggccgca
     1441 cgaccgtata gacgccggtc cgcgtcggat agcggcccgt catcagggcc gaacgcgtcg
     1501 gcgagcagac cggttggacg tagaacgaat cgagaatcgt cccgcccgcc gccagcttat
     1561 cgagctgcgg cgtccgaatc tccttcgagc ccataaagcc gacgtcggcg tagcccaagt
     1621 cgtcggccag gaggaagacg atgtgcggct tgggcggcgc ggtctcggca gcgagctgcg
     1681 ccgacgaaag agcggcgatg aagccggcga tcagagagag acgccacaag tgggctgcgg
     1741 tcatggtttc ctcgcttacg cgtcgggttg ttatgcctgt ttcgccttgg ccttcttggc
     1801 tttggccggc ggctcgttct gctgggccgg gtccatccgc ttgcgaagcg cctcatccgg
     1861 accgccgccg aactgcttcc agtacagcgc cttgaacgga ttgatcttcg gcccgatggc
     1921 gtcctcgttg accaagagcg gctgcacgtc gtcccaccac tggtcgtatg cggcgcggag
     1981 tttggcaacg gcttccgggt gctggtcaat gacgttcttg gtctcgcccg gatcggactt
     2041 caggtcgtac agctcgcgat cggccaccag ccgaaagcgg ctgtcgcgga tcgacgagtt
     2101 ggcgtgtttc gactgggccg cctgcccgcg cggccagcgg ccgacgtgcg tcaccagcgt
     2161 gcgatccggc cacttcgcct tgggattctc taagagcggc agcaagctcc gcccctcgac
     2221 ttgcttcgcc agatcgccgt tgagctgcgc gccgacgatc tcggccagcg tcggcagcac
     2281 atcgagatgc gcactcaatg ccgggcaatc gacaccgcct tggaaccgct ctggccagcg
     2341 ccagaacgac ggcgcgtgcg tgccgccgga gtacatcgag cccttgcccg cccgatatcc
     2401 gccgctatac agccgcgtgt gcgtgccgcc gttatcgctg aggaaaatca cgagcgtgtc
     2461 gttctcgatc ccccactcct tgagctttgc cagcgctttg ccgacgttct catcgacgtt
     2521 ggtgatcatg gcgtagtagg ccacgtcgcc ctcctctagc tgcttgccgt cgatctcctt
     2581 gcctgtgtag agcttgtcgt actgcgctcc cgggctgatg agcggcccgt gcggcgcgtt
     2641 gggcgtgata taggcgaaaa acggagcatc tccttttgtt ttgcccgcga tccactccag
     2701 cgcttggcta aagaaaacgt cggtgcaata ccccttcgtc ttcacgaacg tgccgttgtg
     2761 caggatcgcc gggtcaaaat acttgttgtc cggcgcatcg ccgccgctgc cgggatacgt
     2821 ttgcccgatg ccccccgcgc catggataaa cacctcgtcg aagccgcgat tctgcggctg
     2881 gtagggctct tcgtccccca gatgccactt cccgaagatg cccgtcgcat agccggccga
     2941 tttcagcact tgcgcgaggg tcgtcgcctt cagcgtcatt cgctcgcgct ccaggatcgt
     3001 gtgcgtgatg ccgttcttga actcgtgccg cccggtcatg agcgccgaac gggtcggggc
     3061 acaagtcggg ctgacgtgaa aatcggtgaa ccgcacgctc tcgcgataca gccggtcgag
     3121 gttcggcgtc tggatcagcg gattgccatg ggcagcgata tcgccccatc ccatgtcgtc
     3181 gggcatgatc aggacgatgt tcggcagcga ggcgcccggc gcggcccggc tggcgtgcgt
     3241 cattgggagc atggcgccga aaacagcgca ataccaaatt gcccgctgca tcgttcgttg
     3301 catcgaccgt cgtgaagtca tcgtttcctc cgccttgacg tgactgaata gccgccggcc
     3361 gccgcaggcg acgaagcgca gtttaggctt tgtttgaaaa cacatgaaga cgtaggggtg
     3421 cctggggttg agcgatagct ctaccccagc agtccgggtt attgcttcgc aaaaacccgg
     3481 ccaccctgac cggccaccct gacgcgatct aatggggctc gccgaggctg tggatcgtgt
     3541 tgtgaatcgt ccgctcaaac gcctggccgg aagtagcgcg cagcgatatt ttaatctcca
     3601 tgccccacgt cgggtgcagc tcgggaatgg ttagctgaat ggttttgccg tcgctcgcaa
     3661 ggctcgcggc gtccacacgc agcttgcgct cgtcgaaatg cttcgacccg tagttctccg
     3721 tccgtttcag cgaccacgtt tggaccgcga agttgtcgag cgcggcgacc gattgcggat
     3781 cgacgggatc gctgagctgg atcgtgacgg cgcccgcgcg gcagtgcagc tcgagcggca
     3841 cgtgcatcgg ccggccggtg gcgcggatgc gatacagccc gcccggctgc gtggcgctgc
     3901 cggcccaggc gaacatgccg caaacataga ggcgcccgtc ctgcggatga aaccgcccgc
     3961 gcatgattcc ggtcggaaac gcgggcagcg gcaactcgca catacccccc tgcatcagtc
     4021 cgccgacgcg ctcgtgcggc acgacgaaca ccttgccgtt gccgtacgac aggttcagaa
     4081 gcgctccatc gagcggcccc cagcttttgc tgttcaccca cagcaattcc gccggcgagc
     4141 gatcgaagct gttggtgatc cagcacagcg ggggctccat cgccgaatct gacgagtcgg
     4201 tcacgtcgtg atagccgaac atgttgccgt aaaacttggg ccggcccgag ggatcgagtg
     4261 tgacccagtt gatgcggttc ttcgggttcc agtgcccttc ctgatcggtc acgacgaacg
     4321 acccgtcggg gttcaggcac accccgttcg ccgcccgaaa gccgttggcc aggatgtcgg
     4381 tgcgcaagcc gtcgcgactg acgcgcagca gcgtgccgtg gtgcggcacg agggccggca
     4441 gcgcgtgacg ggccgacttg gcatagtaga gattgccgtc cgcatcggcc tgcaggccca
     4501 tcgcaaactc gtggaaatgc tccgtcacct ggtggtcgtt gttgaagcac tcgtaaaaat
     4561 cggtttcgcc gtcgccgttc aggtcgcgga ggatgacaat ctgatcgcgg cacgtgacgt
     4621 agatctggcc ttcgatgacc ttcagcccca gcggctggaa gagcccggag gcgatgcgtt
     4681 gccacgtgag cgtggctggc gcgcccgcgg aagccgccgc ggaatccggc gcggcagcgc
     4741 gcagcccgct cagcagccac acgtccccat cccacgagca caccgccgcg cggtcgccgt
     4801 cggcaaagaa atcgagcccc gtcagccgcg tctgggccag ccatggattc gcgtcgggcg
     4861 gcgtcagcac atcgaccgcg aagggaccgt cgtcccggcc gcgtttca
//
LOCUS       DASUGB010000167         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_19646,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000167 DASUGB010000000
VERSION     DASUGB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19646"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFV87_03280"
     CDS             complement(<1..777)
                     /locus_tag="VFV87_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5102803.1"
                     /translation="MALLEIRDVTRRFGDFTAVDNVSLSIEAGEFFCLLGPSGCGKTT
                     LLRMVAGFDAPNGGSIWLDGKDMTGVPPEKRNVHTVFQSYALFPHMTVADNVSFPLRM
                     AGLEPASIKTRVDEALDDVALTGFSSRYPNELSGGQRQRVAVARALINRPKLLLLDEP
                     LAALDAKLKENVQLELITLQKEVGIAFVYVTHDQQEALALAHRIAVMNHGRVEQLDEP
                     SRIYGFPKNRFVADFIGTCNILEAEVARIDAGRMQLRVPGL"
     gene            1192..>2141
                     /locus_tag="VFV87_03285"
     CDS             1192..>2141
                     /locus_tag="VFV87_03285"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX5102804.1"
                     /translation="MVIRPKPERWTRLFAAIGAAALFGLCATSAHAQSEILDELLEKL
                     KDKGVLSGDEYQALKKAREDEMAQQRAERRRQAVKQAQDAEKEQKAQDAAAKATKFDV
                     NPGIKTMQLYGDVRLRFEARDGYSPSPSGLGVSGTNDVSRERWRYAARIGIRGDLTDD
                     WFYGLRLDTGQNNRSAWVTFGGDTNLGTNGQGPSAKNDDGVQIGQVYMGWRATPWLTL
                     QAGKMPNPFYTTPMVWDPDIQPEGAAERLSYKFGDSLELFGNFAQMIYQDVNPDQTKG
                     VDLGFHKKDAFQFGWQGGAIYKFSPEMNVKGAVTYYNYVGL"
BASE COUNT          436 a          655 c          676 g          374 t
ORIGIN      
        1 gagtccggga acgcgcagct gcatgcgccc cgcatcgatg cgggcgacct cggcctcgag
       61 gatattgcag gtaccgatga agtccgcgac gaaccgattc ttgggaaatc cgtaaatcct
      121 ggacggctcg tcgagctgct cgacgcggcc atggttcatg accgcgatgc gatgcgccag
      181 cgccagtgcc tcctgctgat cgtgcgtcac gtacacgaac gcgatgccga cctccttttg
      241 cagcgtgatc agctcgagct ggacgttttc cttgagcttt gcatcgagtg ctgccagcgg
      301 ctcgtccagc aacaacagct tcggccgatt gatcaaggca cgggcgacgg ccacccgctg
      361 ccgctggccg cctgaaagct cgttcggata acgactgctg aagcccgtca acgcgacatc
      421 gtcgagcgcc tcatccacgc gcgtctttat gctggcaggc tcgaggccgg ccattctcag
      481 cggaaagctg acgttgtccg ccaccgtcat gtgcgggaac agcgcgtagc tttgaaacac
      541 ggtatggacg ttccgcttct ccggcgggac gcccgtcatg tccttgccgt ccagccagat
      601 gctgccgccg ttgggggcat cgaagccggc caccatgcgc agcaacgtgg tcttgccaca
      661 gccggacggt cccagcaggc agaagaactc gccggcctcg atgctgagac tcacgttgtc
      721 gaccgccgtg aaatcgccga agcggcgggt cacgtcgcga atctcgagca gggccatcga
      781 gaagggaatc gtcaggggat atcgaaggaa gccgcgattc tagcaaatga cgcggagcga
      841 taaatccttg acgcgcaagg agccgggcgg tggcactggc acgcctcggt gccgcggaat
      901 gtcgcgctag acctttgacg acggtgctcg ggccaaggga ccgcgcccct cgggcgctga
      961 tccattcggc tcctccaacc acatgcggct tccggtttgt tcgcctttgc aagcggcccg
     1021 tccgtgcatg agcgacccgg ccctcattaa tcaaactgtc acgttggttc cggacaatgc
     1081 cgcacgctga ggcaaggcgg ctcgttactc agggagaggc caacagcagg agcgccgccg
     1141 gcctcggtac gtcatcgaat gcgttccgca atgatcagga gacaagacta aatggtgatc
     1201 cggcccaagc ccgagcgttg gacccggttg ttcgcagcga tcggtgcagc cgcactgttc
     1261 ggcctgtgcg cgacctcggc ccacgcccag agcgaaatcc tcgacgagtt gctcgaaaag
     1321 ttgaaagaca aaggcgtgct gtctggggac gaatatcagg ctttgaagaa agcccgcgaa
     1381 gacgaaatgg ctcaacagcg tgccgagcgc cgccgccagg ccgtgaaaca ggctcaggat
     1441 gccgaaaagg aacaaaaggc gcaggatgcc gccgcaaagg cgacgaagtt cgatgtgaat
     1501 ccgggcatca agacgatgca gctctatggc gacgtgcgct tgcggttcga agctcgcgac
     1561 ggctattcgc cttcgccttc gggtctcggc gttagcggga cgaacgacgt gagccgcgag
     1621 cgctggcgct acgcggcgcg gatcggcatc cgtggcgacc tcacagacga ctggttctac
     1681 ggactacgcc tcgacacggg tcagaacaac cgttcggcgt gggtgacatt cgggggcgac
     1741 accaacctcg gcaccaatgg acagggtccc tccgccaaga acgacgatgg cgttcagatc
     1801 ggccaggtct acatgggatg gcgcgccacg ccatggctga ccttgcaggc cgggaagatg
     1861 cccaatccgt tctataccac accgatggtg tgggatccgg atattcagcc cgagggggcc
     1921 gcggagcggc tcagctacaa gttcggcgac tcgctcgaac tgttcggcaa tttcgcccag
     1981 atgatctacc aggacgtcaa ccccgatcag accaagggcg tggatctcgg tttccacaag
     2041 aaggacgcct tccagttcgg gtggcagggc ggtgcaatat ataagttctc gcccgagatg
     2101 aacgtgaagg gtgcggtgac gtattacaac tacgtcgggc t
//
LOCUS       DASUGB010000168         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_854054, whole genome shotgun sequence.
ACCESSION   DASUGB010000168 DASUGB010000000
VERSION     DASUGB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854054"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2031)
                     /locus_tag="VFV87_03290"
     CDS             complement(<1..2031)
                     /locus_tag="VFV87_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5102805.1"
                     /translation="MKRVILTTVGLLALLASSAPAADDPKGIDFFEKNIRPVLVSKCY
                     QCHSASAKELGGELRLDTKEGVLKGGESGLAVVPSKPAESLILQALRHEDGLEMPPKE
                     KLSDSVVADFTKWIEMGAPDPRKKNVSTVGGKINILEARKFWAFQPPKVVAPVAAQNA
                     AWERSEIDKYILAGLEAKGLSPVADADKATLIRRAYFDLIGLPPSPEQIDAFVNSSDP
                     LAFEKLIDELLAMPQFGERWGRHWLDVARYGESTSKERNIPFPYAWRYRDYVYDSVAA
                     DKPFDQFVREQVAGDLLPAKNDAQRNEMLTATGLLALGPKSLNQRNAEQFRMDTADEQ
                     LDVVSRGVMALSVACARCHDHKFDPIPTADYYAMVGIFRSTDVLGGVKQGNNKAGYAG
                     DFVYLSEPSPKKEIADESTRKELVTLNRQLDTAKEELQKARSELRKKAEAAESNPKKG
                     KAKNAKAAQKQLAKREAAALREHQQKIKELEDKIAALQGGSSGGSGSGIPVMGVRDSN
                     NPEDCRINIRGEVTDLGDSVPRGYVRVLTFPNSPDVVPEQSGRLQLAMWLTSEKNPLT
                     ARVAANRVWYHLFGRGIVSTVDNFGALGEKPTHPELLDYLALKFMQDGWSTKKLIREI
                     MLSRTYQLASDHSEANFAVDPGNTLVWRMNRKRLEAEAIRDSILAVSGNLDLK"
     gene            2606..>2879
                     /locus_tag="VFV87_03295"
     CDS             2606..>2879
                     /locus_tag="VFV87_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX5102806.1"
                     /translation="MPVQMQLSRIIISEINPQQIIYLKEVDGDRTFPILIGIFEAESI
                     NIGVKRLQSPRPRTHELLVKAIEEMGGELDSVVISELRDHIYFARLR"
BASE COUNT          504 a          905 c          855 g          615 t
ORIGIN      
        1 tttcaggtcc agattgccgc tgaccgccag gatcgaatcg cggatcgctt ccgcctccag
       61 ccgcttgcgg ttcattcgcc agacgagcgt attgccagga tcgacagcga aattcgcctc
      121 actgtgatcg ctcgcaagct gatacgtccg actgagcatg atctcgcgga tcagcttctt
      181 cgtggaccag ccatcctgca tgaacttgag ggccaggtag tcgagcagct cggggtgcgt
      241 cggcttttcg cccagagcgc caaagttatc gaccgttgaa acgatcccgc gaccaaacag
      301 gtgataccac acgcggttgg ccgccacgcg ggccgtcagc gggttctttt cgctggtcag
      361 ccacatggca agctgcagcc ggccgctttg ctcaggaacg acgtcgggcg aattcgggaa
      421 cgtcagcacc cgcacgtagc cgcggggaac ggaatcgccc aggtcggtca cttcaccgcg
      481 gatgttgatc cggcaatctt cgggattatt ggaatcgcgg acgcccatca cggggatgcc
      541 ggagcccgat ccgccgctgg aaccgccttg cagggcggcg atcttgtctt ccagctcttt
      601 gatcttctgc tgatgctcgc gcagggcggc ggcttcgcgc ttggcgagct gtttctgagc
      661 ggctttggcg ttcttggcct tccccttctt gggattgctc tccgcggcct ccgccttttt
      721 tcgcagctcg gaccgggcct tttgaagctc ttccttcgcc gtatcaagct gccggttcag
      781 ggtaaccagc tctttccgcg tgctctcgtc ggcgatttct ttcttcggcg acggctcgct
      841 tagatagaca aaatcgccgg catagcctgc tttgttgttc ccctgtttga cgccccccag
      901 cacgtcggtg ctgcggaaga tgccgaccat cgcgtaataa tcggccgtcg gaatcggatc
      961 gaacttgtgg tcgtggcagc gggcacaggc gaccgaaagg gccatcacgc cgcggctcac
     1021 cacgtcgagc tgctcgtcgg ccgtgtccat ccggaactgc tcggcgttcc gctggttcag
     1081 gctcttggga cccagggcaa gcaggcccgt tgccgtcagc atctcgttcc gctgggcatc
     1141 gttcttggcc ggcaacaggt cgcccgccac ctgctcgcgg acgaactgat cgaacggctt
     1201 gtcggcggcg accgagtcat agacgtaatc gcgataccgc caggcgtacg gaaacggaat
     1261 gttccgctcc ttgctggtcg attcgccata gcgggccaca tcgagccagt gccggcccca
     1321 ccgctcgccg aactgcggca tcgccagcag ctcatcgatc agcttctcga aggccagcgg
     1381 atcgctgctg ttgacgaacg catcgatctg ctccggcgac ggaggcaggc cgatcagatc
     1441 aaaataggcc cggcggatca gcgtggcctt gtcggcgtca gcgacaggcg aaaggccttt
     1501 cgcttccagg ccggccagga tgtacttgtc gatctcgctc cgttcccacg cagcattctg
     1561 ggcggcaacg ggcgcgacga ccttcggcgg ctggaatgcc caaaacttgc gggcttccag
     1621 aatgttgatc ttcccgccga ccgtggagac gttcttcttc cgcgggtcgg gcgctcccat
     1681 ctcaatccac ttcgtgaaat ccgccacgac gctgtcggac agcttctcct tgggcggcat
     1741 ctccaggccg tcctcgtgcc gcagggcctg caggatcagg ctctccgcag gcttgcttgg
     1801 cacaaccgcc agaccgcttt cgcccccctt gagcacccct tccttcgtat ccagccgcaa
     1861 ttcgcccccc agttccttgg cgctggccga atggcactgg tagcacttgc tgacgaggac
     1921 cgggcggatg ttcttctcaa agaagtcgat ccccttcggg tcatccgccg ccggagccga
     1981 cgacgccagc aaagccaata gtccaaccgt cgtcagaatc acgcgtttca tcgccaacct
     2041 cccccggcgg tgtgcatccc tcatcgcgag agccacgttc agagcgccct cggcctgaag
     2101 agatagatgc gcgaggctcg caaaatattt ttccagacac acaggggagc gaattcagca
     2161 gatcgcaact tcttaatctg caatgacttg tgtcacgttt agtccttgtt ggacttgagc
     2221 gagaggcggc ttcgacggcg cgcgcttaaa atggaagatg cgctacaggc tgcgaacgct
     2281 gatgatcatt ttggcggtcg ggcccccagt tctggcaggg ctgtggttac tgggcgtacg
     2341 gctgacgagc gaaccggcgt tggtgcaatc cctgggtctg gcgttcactt ttgcctgcgt
     2401 catagcaact ccactgtacg gcctcgcgtt tgtgttttgg gtattcggag cgggcattgc
     2461 caatgctctt tcccgattga tggagcggcg ttgtcgtcgg aaataggtcg cgtggcgttt
     2521 gttgccgttg ccgtcacaag ttcgagctcc gcgtctataa tcgagcttga cagtcgaagt
     2581 agccgccgcg gaacaggaat cgtccatgcc ggtgcaaatg cagttgtcgc ggatcatcat
     2641 cagcgagatc aatccgcagc agatcatcta tctcaaggaa gtcgatggcg accggacttt
     2701 tccgatcttg atcggcatct tcgaggccga gagcatcaac atcggcgtca agcggctgca
     2761 gtcgccccgg ccgcggacgc acgaattgct cgtcaaggcg atcgaggaga tggggggcga
     2821 gctggacagc gtcgtgatca gcgagctccg cgaccacatt tatttcgccc ggctccgcg
//
LOCUS       DASUGB010000169         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_992655, whole genome shotgun sequence.
ACCESSION   DASUGB010000169 DASUGB010000000
VERSION     DASUGB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992655"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFV87_03300"
     CDS             complement(<1..471)
                     /locus_tag="VFV87_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5102807.1"
                     /translation="MSLLADRTRAFDSSGIRKVFDLAAKMKDPINLSIGQPDYDVPEE
                     IKNACIEAIKAGKNAYALTQGMPVLREKLQAQIDAEYKHADRKVFVSSGTSGGLALAM
                     WSLVNPGDEVIVFDPYFVMYTALTQLVGGVPVIIDTYPDFRIDLNRVAAAITPRT"
     gene            complement(648..2804)
                     /locus_tag="VFV87_03305"
     CDS             complement(648..2804)
                     /locus_tag="VFV87_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5102808.1"
                     /translation="MFRCALISYALPLICVAAIAQDAPNAERPQLDTSRGDRMIAEYF
                     RRETAKLQENCLAEIQTLDDWNAKRSEYRRQLLEMLGLDPLPERTDLKPVITGTVEAD
                     EFVVEKLHFQSRPGLYVAGNLYRPKKVDQPLPAILYVCGHGRVVNKDGESLGNKTHYQ
                     HHGAWFARNGYVCLTIDSLQLGEIEGIHHGTHRYGMWWWLGRGYTPAGVECWNCMRAL
                     DYLETRPEVDKTRFGVTGRSGGGAYSWWIAATDERIKCAVPVAGITDLQNHVVDGCVE
                     GHCDCMFMVNTYRWDYPLVAALVAPRPLLISNTDSDGIFPLDGVVRTFDEARRIYRLY
                     DETEKGTSAKVALNITAGPHKDTQELQVHAFRWLNHYLKDDDSLVEDTAVKFFEPEQL
                     KVFDKLPEDQINTEIQESFVARAAPADVPADKAAWETMRDGWLTALREKTFRGLPAAD
                     SPLLGGTPTAHPPAKELWSVEKDGVRFAAYEFTSQEPFALRLYVLHRTGLQSPELSVL
                     NFLDDQGWQEFLADIRPAFEAELKDEQLPPADAEGWADMKQMFASFPWEMTYVCPRGV
                     GPTAWDQSEKKQVQYRRRFYLLGQTLEEMQMYDVRRAIETVQAVPGLEKTPLWLQSHR
                     TMAGITLYASLFESSLFKPDIKRLDLYELPKTNRDGPTLFNVSRYFDMPQVVAMAAER
                     SKIVIYQDEEGWDYPQSVAEKLGWDAKQVQIRKRPK"
     gene            2961..>3439
                     /locus_tag="VFV87_03310"
     CDS             2961..>3439
                     /locus_tag="VFV87_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102809.1"
                     /translation="MFQRIRPRFSLRSLLLLFTLAAVSVSHYVTSGKLEQIRAERDSQ
                     RQTIRDLNDQLGRLTVDDPTQVHAIATFQPWVAPTHYGSDFVQLDGLAWDLHLPAGTE
                     WRLCASFTLIPQIGLPPLQFQRPVKSGVQGDWHLRFSISPSPSRGWTAEFVDGEQTYP
                     "
BASE COUNT          599 a         1116 c         1037 g          687 t
ORIGIN      
        1 cgtccgcggc gtgatcgccg cggcgactct attgaggtcg atccgaaagt cgggatacgt
       61 gtcgataatc accggcacgc cgccgacgag ctgcgtgagc gccgtgtaca tcacaaaata
      121 cggatcgaaa acgatcactt cgtcgcccgg attaaccagc gaccacatcg ccagggccaa
      181 tccgccgctg gtgccgctcg atacaaacac cttgcggtcg gcgtgcttgt actccgcgtc
      241 gatctgggcc tgcagcttct cgcgcagcac aggcattccc tgcgtcaggg cataggcgtt
      301 cttgcccgcc ttgatcgcct cgatgcacgc gttcttgatc tcctccggca cgtcatagtc
      361 cggctgcccg atcgagaggt tgatcggatc cttcatcttg gccgcgaggt cgaagacctt
      421 gcggatgccg gacgagtcga aagcgcgggt gcggtcggcg agcaaactca ttttggattt
      481 taggttttcg attttggatt ccttgttccc aagctccgct tgggaacacc tgtttcgaag
      541 ctcctgcttc gagacaccgg agcggaagca gagcttccag acgtgcggtc ccaagcggag
      601 cttgggaccg cgctcctgct gtcctctgtc ttctgtcctc tccggcccta cttcggccgc
      661 ttgcgaatct gcacctgctt cgcgtcccag ccgagttttt cggcgacgga ttgcggatag
      721 tcccagcctt cctcgtcctg gtagatcacg atcttcgacc gctcggcggc catcgcgacc
      781 acctgcggca tgtcgaaata ccggctgacg ttgaacaagg tggggccgtc gcggttggtc
      841 ttgggcaatt cgtacaaatc gagccgcttg atgtcgggct tgaacagcga cgactcgaac
      901 agcgaggcgt agagtgtgat gcctgccatc gtacgatggc tttgcagcca gagcggcgtc
      961 ttctccagac ccggcaccgc ctgtacggtt tcgattgccc gcctcacgtc gtacatctgc
     1021 atctcctcca gcgtctggcc gagcagatag aaacgccggc ggtactgcac ctgcttcttt
     1081 tcgctctgat cccaggcggt tggcccgacg ccgcgcgggc agacgtacgt catctcccac
     1141 gggaacgacg cgaacatctg cttcatgtcg gcccagccct cggcatcagc cggcggaagc
     1201 tgctcatcct tcagctcggc ctcgaaagcc gggcggatat cggccagaaa ctcctgccag
     1261 ccctgatcgt ccaggaagtt cagcacgctc agctcaggcg actgcaatcc ggtccggtgc
     1321 aagacataga gtcgcagcgc gaacggctcc tggctggtga attcgtaggc ggcgaagcgg
     1381 acgccatcct tctcgaccga ccacagctcc ttggcgggtg gatgtgccgt cggcgtgccg
     1441 cccaacagcg gcgagtcggc agcggggagc ccgcggaacg tcttctcgcg caaagccgtc
     1501 agccagccgt cacgcatggt ttcccacgcc gccttgtcgg ccggcacatc ggccggagcg
     1561 gcccgcgcga cgaacgactc ctgaatctcc gtgttgatct ggtcttccgg cagcttgtca
     1621 aacaccttga gctgctcggg ctcaaagaac ttgacagccg tgtcctcgac gagcgagtcg
     1681 tcgtccttca gatagtgatt gagccagcgg aaggcgtgga cctgcaactc ctgcgtgtcc
     1741 ttgtgcgggc cggccgtgat gttgagggcg accttggcgc tcgtcccctt ctcggtttcg
     1801 tcgtacaggc ggtagatgcg gcgcgcctcg tcgaacgtgc ggacgacgcc atcgagcggg
     1861 aagatgccgt cgctatcggt gttcgaaatc aagagcggcc gcggagcgac cagggccgcg
     1921 accagcggat aatcccagcg gtaggtgttg accatgaaca tgcaatcgca gtgcccctcg
     1981 acgcaaccat cgaccacgtg gttctgcagg tcggtgatgc cggcaaccgg caccgcgcac
     2041 ttgatccgct cgtcggtggc ggcgatccac cagctatagg cgccgccgcc gctgcggccg
     2101 gtgacgccga agcgggtctt atcgacctcg gggcgagttt ccagatagtc cagcgcccgc
     2161 atgcagttcc aacattcgac tcctgccggc gtgtagccgc gacccagcca ccaccacatg
     2221 ccgtagcggt gcgtgccgtg gtggatgcct tcgatttcgc cgagctgcag cgaatcaatc
     2281 gtcaggcaaa catagccgtt gcgggcgaac cacgccccat gatgctggta atgcgtttta
     2341 ttgcccagac tctcgccgtc cttgttgacc acgcggccgt ggccgcagac gtacagaatc
     2401 gccggcagcg gctgatcgac ctttttcggg cgatagaggt tgcccgcgac gtacagccct
     2461 ggccgcgact gaaagtgcag cttctcgacg acgaattcgt ccgcctccac ggtgcccgta
     2521 atgacgggct tcaggtcggt cctttccggc aagggatcca gtcccagcat ctccaggagc
     2581 tgtcggcggt actcgctccg tttggcgttc caatcgtcga gcgtctgaat ctccgccagg
     2641 cagttctcct gcagcttcgc ggtctcgcgg cggaaatact ccgcgatcat ccgatcgccg
     2701 cggctggtat cgagctgggg ccgctccgca ttcggagcat cctgagcgat cgcggcgacg
     2761 cagatcagtg gcaacgcgta ggaaataaga gcacagcgga acatggtggt cccttcgtga
     2821 aagatcgatc ggcgagtctc gtcactatcc tgtgtgcgca ggcggccaat tgcaagccgc
     2881 ggtggtgaac cacgacccga ggacgcgctc cagccgctct gccgagtaga atggttgcgg
     2941 ccagccagca ccgccaatct atgttccagc gcatccggcc tcgcttctcg ctgcgttcgc
     3001 tgctgttgct gttcacgctc gcagcggtgt ccgtttcgca ctacgtgacg agcggcaaac
     3061 tagaacaaat ccgcgcagag cgcgactcgc agcgacagac gattcgggac ttgaacgatc
     3121 aactcgggcg gctgacggtc gatgacccga cccaggtcca cgcgatcgcc acgtttcagc
     3181 cgtgggtggc gcccacacat tacggctccg actttgtcca gttggacggg ctcgcttggg
     3241 atctccacct gccggcgggc acggaatggc gattgtgtgc gtcgttcact ttaattccac
     3301 aaatcggcct tccgccgctg cagttccagc ggccggtgaa gagcggcgtc caaggcgatt
     3361 ggcacttgcg attctcgatc tccccctccc cgtctcgcgg ctggaccgcc gaattcgtcg
     3421 atggcgagca gacctatcc
//
LOCUS       DASUGB010000170         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1965500, whole genome shotgun sequence.
ACCESSION   DASUGB010000170 DASUGB010000000
VERSION     DASUGB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965500"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..720
                     /locus_tag="VFV87_03315"
     CDS             160..720
                     /locus_tag="VFV87_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HEX5102810.1"
                     /translation="MAKRNNHYEAAFEAWLRRLRVPYVAVDETHRSLAGAGSLKSVDF
                     IVSPPGQATTVADSAAPAHWLVDVKGRRFPTGASYWKNWSTADELASLASWETLFGAR
                     FAALLVFAYNVIGSRAPLPADELFVFRGSLYGFVAIRLDHYTSCAQPLSPRWGTVCMP
                     VAHFRALARPAREVLGAPRRVGMGAA"
     gene            complement(787..>2586)
                     /locus_tag="VFV87_03320"
     CDS             complement(787..>2586)
                     /locus_tag="VFV87_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5102811.1"
                     /translation="AARGPSRVLGRPASAYACNSRGASPSSPPDYLLMTRLETLADVA
                     VLHNTYPVPERGLSIGQYLIRRLQDYGIGHVFGIPGDYILTFYGLLEKSPLALVGCTR
                     EDSAGFAADAYARIHGMGCVAVTYCVGGLSVCNSTAGAYAEKSPVVIISGAPGIGERV
                     NNPLLHHKVRDFHTQLEVFEKLCIAGAELDDPVVAFREIDRVLDAAARYKRPVYLELP
                     RDMVDVVPHIAHVYHPTQIEADPQTLAEAVEETAQLLEQARRPILIAGVEIHRFGLQD
                     KVLALAEKHSLPIAATLLGKSVVPEKHPLYIGLYEGAMGHAEVTRFVEDSDCVLLLGA
                     FMTDLNLGIFTAQLDPARCIYVTSEQLRIRHHHYHQVPLAPFLDQLAQRPVDIVRREI
                     PAALRPQSVPFALAPAAELTIRRLIARLDQQIDDNTVVIADIGDSLFAATELTTHGRT
                     EFISPAYYTSMGFSVPAALGALTARPDSRVLVLCGDGAFQMTGLELSTIVRRGLAPIV
                     IVLDNHGYGTERFLHPGDWQFNEVHPWQYHKLPELLGGGTGYEVRTEGEFDEALVRAW
                     NDRHGMSLIQAHIPRDDASDALRRLTERLSKRV"
BASE COUNT          443 a          790 c          897 g          456 t
ORIGIN      
        1 aatcatcgct tagctcgtgg cgggttgaag gctgggcgat cggagagtga aacgggcggg
       61 gaacaaggaa acctcccacc gcggacggcc cgatccaccg ctccagtcca gttgccctgc
      121 tgcacaggct gcgtaccata acacgcctgg ggagctccga tggccaagcg gaataaccac
      181 tacgaggcgg catttgaggc ttggcttcgc cggctgcgcg tgccgtacgt ggccgtggac
      241 gaaacgcacc gcagcctggc cggggcgggc agcctcaaga gcgttgattt catcgtatcg
      301 ccgcccggcc aggccacgac ggtcgcggat tccgccgccc ccgcccattg gctcgtggac
      361 gtcaaaggga ggcggtttcc gactggggcg agctactgga aaaactggtc caccgccgac
      421 gagctggcca gcctggcgag ctgggagacc ctctttggcg ccaggtttgc ggcgttgttg
      481 gtttttgctt acaacgtgat cggcagccga gccccgctgc cagccgatga gctgtttgtg
      541 tttcgcggca gcctctacgg ctttgtggcg atccgcctcg accactacac aagctgcgct
      601 cagccacttt ccccgcgctg gggcacggtc tgcatgcccg tagctcattt tcgcgcactg
      661 gcccggccgg cgagggaagt ccttggcgcg ccgcggcgag tagggatggg ggcggcttag
      721 gcagttccaa gttcgacgtt tcaagttcca ggtttaggtc tcggattgaa acctggaacc
      781 cgaaactcac acgcgtttgc tcagccgttc ggtgaggcgc cgcagggcgt cgctggcgtc
      841 gtcgcgggga atgtgggcct ggatcaggct catcccatgg cggtcgttcc aggctcgcac
      901 gagggcctcg tcgaactccc cttccgtgcg cacttcatag cccgtgcccc cccccaagag
      961 ctccggcagc ttgtggtact gccaggggtg gacctcgttg aactgccagt cccccgggtg
     1021 caggaaccgt tcggtgccgt agccgtggtt gtcgagcacg atgacgatcg gcgcgaggcc
     1081 gcggcggacg atcgtcgata gctccaggcc ggtcatttgg aaggccccgt cgccgcagag
     1141 cacgagcacc cggctgtcgg gccgggctgt gagcgcgccc agcgcggcgg gaactgaaaa
     1201 ccccatcgac gtgtaatacg ccggactgat gaattcggtc cgcccgtggg tggtaagttc
     1261 ggtggccgcg aacagcgagt cgccaatgtc ggcaataaca accgtgttgt cgtcgatctg
     1321 ctggtccagg cgggcaatca gccggcggat ggtgagctct gcggcgggcg ccagggcgaa
     1381 ggggacgctc tgggggcgga gcgcggcggg tatttcgcgg cggacgatgt ccaccggccg
     1441 ctgggcgagc tggtcgagga agggggccag cggcacctgg tgatagtggt gatggcgaat
     1501 gcggagctgc tcgctggtga cgtagatgca gcgggccggg tcgagctggg cggtgaaaat
     1561 gcccaggttg agatcggtca tgaacgcgcc caggaggagc acgcagtcgc tgtcttcgac
     1621 gaaccgcgtc acctcggcgt gccccatcgc cccttcgtac aggccgatat acagcgggtg
     1681 tttctcgggc acgacgctct tgcccaggag cgtcgcggcg atgggcaggc tgtgcttctc
     1741 ggccagagcc aggaccttgt cttgcaggcc gaagcggtgg atttccacgc cggcgatgag
     1801 gatcgggcgt ctggcctgct ccagaagctg ggcggtctcc tccacggcct cggcgagcgt
     1861 ctgtggatcg gcctcgatct gggtcggatg gtaaacatgg gcgatgtgcg ggacgacatc
     1921 gaccatgtcc cgtggcagct cgagataaac gggtcgcttg tagcgcgcgg ccgcatcgag
     1981 cacccggtcg atctcgcgaa acgctacgac gggatcgtcc agctcggcgc cggcgatgca
     2041 cagcttctcg aacacttcga gctgagtgtg gaagtcgcgc accttatggt gcaatagcgg
     2101 attgtttacc cgctcgccga tgcccggcgc gccggaaatg atcaccacgg gcgatttctc
     2161 ggcatacgcg ccggcggttg aattgcacac ggacaggccc cccacgcagt aggtgaccgc
     2221 cacgcagccc atgccatgaa ttcgtgcata agcgtcagct gcaaagccgg ccgaatcttc
     2281 gcgggtgcag ccaacgagcg cgagcgggct tttttcgagt aagccataaa acgtcaagat
     2341 gtagtcgccc ggaatcccga agacgtgccc gatgccgtaa tcctgcaagc ggcggatcag
     2401 gtattgcccg atcgacagcc cccgctccgg tacgggatac gtgttgtgca gcacggccac
     2461 gtcggcgagc gtttccaggc gggtcataag caagtagtcg ggaggcgagg atggggatgc
     2521 accgcggcta ttgcaagcat acgcgctggc tgggcggccg agcacccgcg aagggccgcg
     2581 ggcggc
//
LOCUS       DASUGB010000171         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1826533, whole genome shotgun sequence.
ACCESSION   DASUGB010000171 DASUGB010000000
VERSION     DASUGB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826533"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFV87_03325"
     CDS             <1..333
                     /locus_tag="VFV87_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX5102812.1"
                     /translation="GRGLLTMLVANPAAVRPVQTKAIELTAGEPSYLLFDWLTELLFA
                     FESDKLLLCEFDVQVAGGKLTATCRGEPMDPSRHEMDHEVKAITYHGLTVEQRADRTW
                     QADVIVDI"
     gene            643..2106
                     /locus_tag="VFV87_03330"
     CDS             643..2106
                     /locus_tag="VFV87_03330"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5102813.1"
                     /translation="MKREAFYGPLEKVGDCCWRIPKSYNAGMRVDGLVFADDELLDLI
                     RQDQALEQVVNVAFLPGIQQASLAMPDIHWGYGFCIGGVAATDPDEGGVISPGGVGYD
                     INCGVRLVRSSLFNDDVQPKLTKLMDQLYHAIPAGVGQDGKYTFGQMELRQLMGEGVK
                     FLQARGLATNDDIEHTEARGKLADAQPKNVSQRALERGAKQCGTLGSGNHFLEVQVVD
                     EVHDQEAARVMGLEKDQVCVMIHSGSRGLGYQVCDDALRSLRSAPAKYGIRLPDQQLV
                     CAPVNSPEGEEYLGAMCAAANYGFCNRQLLMWQAREAFEKVFNRGWEHLHMTLVYDVA
                     HNIAKLEEHEVGGVKKRLWVHRKGATRAFPAGHPEVPEMYRDIGQPVIIPGDMGRASW
                     VLAGEAGSMAQSFGTCCHGAGRVMSRTQAVKHAQGRRIDEELAKKGIIARARSWKGLA
                     EEQPDAYKDVDLVVEVVHRAGLARKVARMRPIGVVKG"
     gene            2483..3094
                     /locus_tag="VFV87_03335"
     CDS             2483..3094
                     /locus_tag="VFV87_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5102814.1"
                     /translation="MRRWRKYPSPNLVDLSAFRADLHAHLPWNSTDGKYGPDLHRLAR
                     RLQDEGYFIAESVDEEHNRVLREVAERRGQDKFRNALIAAYDGHCAITGCDAVPALEA
                     AHITPFGGAGSDDVRNGLLLRADIHTLFDLNLLGIEPESLAITLAERLLGTFYADLSD
                     RKLLTPVDSSAEPNFEALRDRCDEFRDCNYGRLHMNSSVIAKR"
     gene            3163..>3626
                     /locus_tag="VFV87_03340"
     CDS             3163..>3626
                     /locus_tag="VFV87_03340"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5102815.1"
                     /translation="MLESLGGVDPARIRIEPPMGTATVEDVIEIEERENRLFELVDGI
                     LVEKVMGYTASRIASRLIAALEAYVDANDLGAVTGEGGMFRMPENLVRIPDVAFAGWD
                     QFPNHELPAEGAPEIVPDLAVEVLSKGNTKAEMARKLREYFAVGVTTVWFVDP"
BASE COUNT          783 a         1043 c         1142 g          658 t
ORIGIN      
        1 ggccgtggcc tattgaccat gctggtggcg aacccggctg cggtgcgacc tgtccagacg
       61 aaggcgatcg agctcaccgc cggcgagccg tcgtacctgc tgttcgactg gctgaccgag
      121 ctgctatttg cttttgagtc cgacaagctg ctcctgtgcg aattcgacgt acaggttgca
      181 ggcggcaagc tcaccgccac ctgccgcggc gagccgatgg accccagccg tcacgaaatg
      241 gaccacgagg tcaaggcgat cacgtaccac ggcctgacgg tcgagcagag ggccgacaga
      301 acctggcagg ccgacgtcat cgttgacatt taggccgccg ttataatagt gaacattgga
      361 acgccatttg cagtgaacac ttggttcgct cgaaggcggc cagattggca gacccgggcc
      421 gccagcacgt actagcagcc ggaagtcaca aaactcgagg cggaggcacg aagcgcaaca
      481 gcgcaacatt gaattgcgcg gccgagaatg attccaagtc gcgtaattgc aaaaggttac
      541 gctcgcaaac gcaaggttga tgcgccacga aaaaaagtca agaagttgcg cgtttttggt
      601 cagtcaaaag gttgcgcggt cgcacgttcg gagcaaacag ccatgaaaag ggaagccttt
      661 tacggaccgc tagaaaaggt gggcgactgc tgctggcgga ttccgaaatc gtacaacgcc
      721 ggaatgcgcg tcgatgggct cgtctttgcc gacgacgagt tgctcgacct gatccgccag
      781 gatcaggccc tggagcaggt cgtcaacgtc gcgttcctcc ccggcatcca gcaggccagc
      841 ctggcgatgc ccgatatcca ctggggctac ggcttttgca tcggcggcgt ggcggcgacc
      901 gatccggacg aagggggcgt catttcgccc ggcggcgttg gctacgacat caactgcggc
      961 gtgcggctgg tgcggtccag cctgttcaac gacgacgtgc agccgaagct cacgaaattg
     1021 atggaccagc tctaccacgc gattccggcg ggcgtcggtc aggacggcaa atacacgttc
     1081 ggccaaatgg agttgcggca gctcatgggt gagggcgtga agttcctgca ggcccgcggc
     1141 ctcgcaacga acgacgacat cgagcacacc gaagcccgcg gcaagctggc cgatgctcag
     1201 ccgaagaacg tcagccagcg ggccctggag cgcggcgcca agcagtgcgg cacgctcggc
     1261 tcgggcaacc atttcctgga ggtgcaggtc gtcgatgaag tccacgacca agaggcggcc
     1321 cgcgtgatgg ggctggagaa ggaccaggtc tgcgtgatga tccacagcgg ctcgcgcggg
     1381 ctgggctatc aggtctgcga cgacgccctg cgctcgctcc gcagcgcgcc ggccaagtac
     1441 ggcatccgcc tgcccgatca gcagctcgtc tgcgctcccg tcaacagtcc agaaggcgag
     1501 gaatacctcg gcgcgatgtg cgccgcggcc aactacggct tctgcaatcg ccagctcctg
     1561 atgtggcagg cccgcgaagc gttcgagaaa gtcttcaacc gcggctggga gcacctgcac
     1621 atgacgctgg tctacgatgt agcgcacaac atcgccaagc tggaagaaca cgaggttggc
     1681 ggcgtcaaga aaagactctg ggtccaccgc aagggcgcca cccgcgcttt ccccgctggc
     1741 catcccgaag ttcccgagat gtaccgcgat atcggccagc cggtgatcat tcccggcgat
     1801 atgggccgcg cgagctgggt cctcgcgggc gaggccggca gcatggcgca gtcgttcggc
     1861 acctgctgcc acggcgccgg ccgcgtgatg agccgcacgc aggcggtcaa gcacgcccag
     1921 ggccgccgca tcgacgaaga attggcgaag aaagggatca tcgcccgcgc ccgcagttgg
     1981 aagggtctcg ccgaagagca gcccgacgcc tacaaggacg tcgatctggt cgtcgaagtc
     2041 gtccaccgcg cgggcttggc tcgcaaagtc gcccgcatgc ggccgatcgg agtggtaaag
     2101 ggttaacaaa gccgcgacca gtggtcgcgg tctgtgagga gttgaagatc atctggccgg
     2161 cccttggtta tattgcattg cgtcggggga aggtgacttt gaattcgacg ggaagcaacc
     2221 agccatccca gtcaatatcg aggcgtacgg taaactcgtt gagacgggag aaatacggaa
     2281 ggatccgtgg tggcacatca aagtcaacgg cgcgaaagtc gaggttggta atgaagtatt
     2341 catctacacc ggcgatagag atcgcgggat cattggggta gcgaccgtcg aggaagttcg
     2401 gtcagaccag cgatcaatcc gcctggaatt tgatctggac aagtgcaaat cgctgctgag
     2461 agaaccgatc tcggcactcg tcgtgcgccg ctggcgaaaa taccctagcc caaatcttgt
     2521 ggatttgagc gcgttcaggg cggacctgca tgcccacctt ccatggaatt caacggacgg
     2581 gaagtacggt cctgacctgc atcgcttggc aaggcgtctc caagatgaag gctatttcat
     2641 cgcggaaagc gtcgatgagg aacacaatcg cgtcttgcga gaggttgccg aacgcagagg
     2701 ccaggacaag ttccgaaacg cgctaattgc agcctatgat ggccattgcg cgatcactgg
     2761 ttgcgatgcg gtcccggcgc tcgaagcagc ccacatcacg ccatttggcg gggcaggctc
     2821 agatgatgtt agaaacggcc tactccttcg cgctgatatt cacacgctgt tcgatcttaa
     2881 ccttctcgga atcgagccag agtcgcttgc cataactttg gcggagcgat tgctgggaac
     2941 tttctatgcc gacctttctg accgcaaatt gttgacccca gtcgattcat ctgcggaacc
     3001 aaacttcgag gcgctgcgcg accgctgcga cgaatttagg gattgcaact atggtagatt
     3061 acacatgaac tcatcagtca tcgctaaaag gtgacgttcc aagggagcac aaacatgctc
     3121 gcaattgtga ccgcgccgcc ccgttttcgg aacttggcgg acatgctcga gagtctcggc
     3181 ggcgtggacc cggcaagaat tcgcattgaa ccgccgatgg gaaccgccac cgtggaggac
     3241 gtcatcgaga ttgaggagcg agagaaccgg ttattcgagt tggtcgacgg cattctggtg
     3301 gagaaggtca tgggttacac cgcgtctcga attgcctctc gattgatcgc agcattggaa
     3361 gcctatgttg atgctaacga cttgggagcg gtgaccggcg aaggtggcat gttccgaatg
     3421 ccggaaaacc tcgtgcggat tcccgatgtg gcgtttgccg gttgggacca gttcccgaac
     3481 cacgaattgc ccgctgaagg cgcgccagag atcgtccccg atctcgccgt cgaagttctc
     3541 agcaagggga atacgaaggc agaaatggct cgcaaactac gcgagtattt cgccgtcggc
     3601 gtgacgacag tctggttcgt cgatcc
//
LOCUS       DASUGB010000172         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1549028, whole genome shotgun sequence.
ACCESSION   DASUGB010000172 DASUGB010000000
VERSION     DASUGB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549028"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VFV87_03345"
     CDS             <1..1384
                     /locus_tag="VFV87_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102816.1"
                     /translation="PTEESLAAALADAEQSLRIAESAVRAAEAALVSVQARIAADAAN
                     YASPPIETAKELSLAAGAEERRHAVLAGQLALQQADLKLVQAKRSVKAGDMASEKAAK
                     EAETAQANAAKALEAAQKAAGEPTENYTRLTPVHPATSTGRRLALARWITSRENPLAA
                     RVAINHLWLRHFGSPLVPSVFDFGMNGKPPTNQPLLDWLAVELMDSGWQMKQIHKLMV
                     MSRTYRLASTQGNGGQGTGDREQGGAVPNRQSAIRNPQSTDPENLFYWRANPRRMEAE
                     IVRDATLYVAGSLDPKLGGPDLDQASGMTVPRRSLYFRASKEKKMEFLSLFDSPNPVE
                     CYRRSESIAPQQALAMANSPLTLAQSRVLAKKLSDGLGNVAAEEAPKRFIEVAFVRIL
                     CREPTADELAACAEFLTEQSAKLSDPKSLTAFSAGPAASVPPSTDPQQRARENLVHVL
                     LNHNDFVTVR"
     gene            1412..2866
                     /locus_tag="VFV87_03350"
     CDS             1412..2866
                     /locus_tag="VFV87_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102817.1"
                     /translation="MTNHRHRRAFLADLGMGFAGVAMGAMLAREGNVRAAEPDREAHA
                     SRSPAKAKSVIWIFLVGGMSHMESFDPKTELNAHAGKTIAESPYKGLLDSPYLKKNLR
                     EFVEGLHKVQPTIFPMQIGFQKHGQSGIEISDWFPHLARHVDDLAVVRSMWTTDNDHG
                     AQLQFHTGRHAIEGPFPTIGSWVHWGLGSLNDNLPSYCVLGTPLADCCGGVQGHGANY
                     LGPEHAGIRLATDPAAPLPFAQPDQTVFREEQAREFELLGKLNQLAGIEYPDDPALSA
                     RIRSYELAFQMQKAVPEAVALGTESAATHAAYGLAQNETKTFGQQLLVARRLVERGVR
                     FVQVFHGSNGGAGAWDAHSGLKAGHSRLAKEIDQPIGALLQDLKQRGLLDETIVVIGT
                     EFGRTPGAERSDGRDHHPYGFSVALAGGGIRGGVVHGATDELGFHAAENRHYVTDIHA
                     TVLDLLGLDHRRMEIPGRKRLDLDFGQPIREIMV"
     gene            complement(2863..>3491)
                     /locus_tag="VFV87_03355"
     CDS             complement(2863..>3491)
                     /locus_tag="VFV87_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102818.1"
                     /translation="MKSLVGLGVSLYACLFVLTACAPPAEQGQSNPAAPPAAPPAPPP
                     TGEQVAAAPPAAPPVAPAPAQPAEAPAANPLAALSPLAAYEDLDRRINAVLSKITDGP
                     SANAAADELAPLTAEMKLKLRPYIVAFATMSDAEQLAYVQRQTEEAISQKSAGVQVDH
                     RQLIELAREPGNERFKASLQAMFQTMIDEGTTGMRRSMSQKLERLNRP"
BASE COUNT          644 a         1136 c         1156 g          555 t
ORIGIN      
        1 gccaaccgag gagagcctgg ccgcggcact ggccgacgcg gagcaatcgc tgcgaatcgc
       61 cgagtcggcg gttcgagccg ccgaagccgc gttggtttcc gttcaggctc ggatcgcggc
      121 cgacgcggcc aactacgcgt cgccgccgat cgaaacggca aaagagctgt ccctggcggc
      181 tggagcggaa gaacgccggc atgcggtgct cgccgggcag ctcgccctgc agcaagccga
      241 tctgaagctc gtgcaggcga aacgcagtgt caaagccggc gacatggcga gcgaaaaagc
      301 ggccaaagag gccgaaacgg ctcaggccaa tgccgccaaa gccctcgaag ccgcccagaa
      361 ggccgccggc gagccgacgg aaaactacac gcgcctgacg cccgtccatc cggccaccag
      421 cacgggccgg cggctggcac tggcccgctg gatcacgtcg cgcgagaacc cgctggcggc
      481 ccgcgtggcg atcaaccacc tgtggctccg ccatttcggc tcgcccctgg tgccgagcgt
      541 cttcgacttc ggcatgaacg gcaagccgcc gacgaatcag ccgctgctcg actggctcgc
      601 cgtcgagctg atggacagcg gctggcagat gaagcagatt cacaagctga tggtaatgag
      661 caggacgtac cggctggcgt ccacgcaagg aaatggggga caggggacag gggacaggga
      721 acaagggggc gctgttccca atcggcaatc cgcaatccgc aatccgcaat cgaccgatcc
      781 cgaaaacctc ttttactggc gggccaaccc gcggcggatg gaggcggaga tcgtgcgcga
      841 tgcgacgctg tatgtcgcgg gatctctcga tccgaagctc ggcggaccgg acctcgacca
      901 ggcctcgggc atgacggttc cgcggcgaag tttgtatttc cgggcgagca aagagaagaa
      961 gatggagttc ctgtcactgt tcgacagtcc caacccggtc gaatgctatc gccgcagcga
     1021 gagcatcgcc ccgcagcagg ccctggcaat ggccaacagc ccgctgacgc tggcccaatc
     1081 gcgcgtgctg gccaaaaaac tgagcgatgg cctcggcaac gtcgccgccg aagaagcgcc
     1141 aaaacgtttc atcgaagtcg ccttcgttcg catcctctgc cgcgagccaa ccgccgatga
     1201 actggccgcg tgcgccgagt tcctcaccga acaatcggcg aagctctccg acccaaagtc
     1261 gctcaccgcc ttctccgccg gcccggcggc ctccgtgccg ccctccaccg acccgcagca
     1321 gcgggcgcgg gagaatctcg tccatgtcct gctcaatcac aacgattttg tgaccgtacg
     1381 ataggctgac aggaagattg aggacaggaa gatgacgaac caccgacatc gacgcgcatt
     1441 cctcgccgat ctcgggatgg gcttcgcagg ggtggcgatg ggggcgatgc tggcgcggga
     1501 gggaaacgtg cgcgcggccg agcctgaccg ggaggctcac gcctcccgct cgccagcgaa
     1561 ggcgaagtcg gtgatctgga ttttcctcgt cggcggcatg agccacatgg agtcgttcga
     1621 tccgaagacg gagctgaatg cgcatgccgg caagacgatc gccgaaagcc cgtataaggg
     1681 cctgctcgat tcgccgtatc tgaagaaaaa cctccgcgag ttcgtcgagg gcctgcacaa
     1741 agtgcagccg acgatttttc cgatgcagat cggatttcag aagcacggcc agagcggcat
     1801 cgagatcagt gactggttcc cgcatctggc gcggcacgtg gatgacctgg cggtcgtccg
     1861 cagcatgtgg acgaccgaca acgaccacgg ggcgcagctt cagttccaca ccggccgcca
     1921 cgcgatcgaa gggccattcc caacgatcgg ctcgtgggtg cattgggggc tgggatcgct
     1981 gaacgacaat ctgccgtcgt attgcgtgct cggcacgcca ctggccgatt gctgcggcgg
     2041 cgtgcaggga cacggggcga actacctcgg gcccgagcac gccggcatcc gtctggcgac
     2101 ggatcccgct gcgcccctgc cgttcgctca gccggaccag acggtgtttc gcgaggaaca
     2161 ggcccgcgag ttcgagctgc tgggcaagct gaaccagctt gccgggatcg agtatcccga
     2221 cgatccggcg ctctcggccc gcattcgcag ctacgagctg gcgttccaga tgcagaaggc
     2281 ggtgcctgag gcggtcgcgc tcggtacgga atcggccgcg acgcatgccg cctatggcct
     2341 cgcgcaaaat gagaccaaga cgttcggcca gcagttgctc gtcgcccgac ggctggtgga
     2401 gcggggcgtt cggttcgtgc aggtcttcca cggctctaac ggcggggccg gagcgtggga
     2461 tgcccacagc ggcctcaaag cgggccattc ccggctcgcg aaggagatcg atcagccgat
     2521 cggtgctttg ctgcaggacc tcaagcagcg cggcctcttg gacgaaacga tcgtcgtcat
     2581 cggcacggaa ttcggccgca cgcccggcgc cgagcgcagc gacggccgcg atcatcatcc
     2641 ctatggcttt tcggtcgcgc tggccggcgg cgggatccgg ggcggcgtcg ttcacggcgc
     2701 gaccgacgag ctcggctttc acgccgccga gaaccggcac tacgtgacgg acattcacgc
     2761 caccgtgctg gaccttctcg gcctcgacca ccgccggatg gagatccccg gccgcaagcg
     2821 gctcgacctc gatttcggcc aaccgatccg cgaaatcatg gtttaaggcc ggttcagccg
     2881 ctccagcttc tggctcatgc tgcggcgcat gcctgtcgtg ccttcatcga tcatggtctg
     2941 gaacatcgcc tggagcgacg ctttgaagcg ctcgttgcct ggctcgcggg ccaactcgat
     3001 gagctgccgg tgatcgacct ggacgcccgc agatttctga ctgatcgcct cttccgtctg
     3061 gcgctgcacg taggcaagct gctccgcgtc cgacatcgtg gcgaacgcga caatgtaggg
     3121 acgcagcttg agcttcattt cggcagtcag aggcgccaat tcgtcagccg cggcattggc
     3181 cgaaggaccg tcggttattt tgctgagcac cgcgttgatc cgccgatcga gatcctcgta
     3241 agcggcgagc ggagagagag cggccagcgg gttggcggcc ggcgcttccg ccggctgagc
     3301 tggtgcgggc gctacgggag gcgctgcggg aggtgctgct gcaacttgct cacccgttgg
     3361 cggtggcgcc ggcggagcgg ctggcggcgc agcgggattc gactgccctt gctccgctgg
     3421 cggcgcgcag gctgtcaaca cgaacagaca ggcatatagg ctcaccccca gccccacaag
     3481 cgatttcatg g
//
LOCUS       DASUGB010000173         9368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1965905, whole genome shotgun sequence.
ACCESSION   DASUGB010000173 DASUGB010000000
VERSION     DASUGB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965905"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="VFV87_03360"
     CDS             complement(<1..1743)
                     /locus_tag="VFV87_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5102819.1"
                     /translation="MKPGNAFCARLGPPLRFLIAAPLIWAALAASAWGQASRTAQNDT
                     DDDKDRLATPYAEIVRADQPVAWWRFDDGQPAGELNGQPWQAAGVEGPAKLAQPGSPA
                     AKFPLFAGHNQAVLFSAPASLRYDDPGVDSPLDFAQGDTITLEAWVSPAKLEGGQQIY
                     VVGKGRTGNTGFAADNQNWALRLAGQGGSCRISFLFRGAENRKGNSDDWHRWTSDSGF
                     AAGSGWHYVAVSYTFGKGDSVRGYVDGRESKGTWDYGGKSDEAPVVDDDQVWIGSASK
                     NNPGNSFAGGIDEVAIYRTALSAERIAARWKAILPKPYVTNVPLPKDEVLVEVLEGLP
                     DAWTWDFVPPTPSERFTQAELAFVEVPRKYTNHGVIDDRSSPFVLWAHTQIDLPAGKH
                     RLLLRSRHAARLYIDDKLAVENPFPNGKTDGHNPWEPVESKISSRIRPLQPGDQEKDA
                     EIELAGGPHHFRLEIFVGGKKRRPELGETSVAIAPAGTDDFHVLTATASFPLTDEAWK
                     PWEEQTRANLVRTNQARRAAASADYAQYWDRRHDFARHYAVGRVSNPSADSIDQFIDA
                     TLAQQALKPAPLTGD"
     gene            2063..2434
                     /locus_tag="VFV87_03365"
     CDS             2063..2434
                     /locus_tag="VFV87_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5102820.1"
                     /translation="MNKKESKPFKNLLLGLRSRLRGDVNGLADAALRKTRAESSGDLS
                     SMPIHMADLGTDAFEQEFNLSLIEHDGSALEQIEAALERIEDGVYGVCTECEGKIPKA
                     RLEVLPHTPHCVKCAEKVTAR"
     gene            2499..3125
                     /locus_tag="VFV87_03370"
     CDS             2499..3125
                     /locus_tag="VFV87_03370"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5102821.1"
                     /translation="MPRNRYVTYLAIALAGFAADLATKEATFRWLGVHPHFVAADDPA
                     AIARWRGDPGLNHLWWLWEGRLGIQTSLNEGALFGMGAGYWWLFATLSIVALAGVSTW
                     LFAFKAALDRWINVALAFVTAGILGNLYDRLGLFDSTGLDAKFHHAVRDWILFVWPES
                     GMKMFNPWPNFNIADSLLVTGAIMLVVHAVVWREKKEDASDKSERTAA"
     gene            complement(3122..4252)
                     /gene="mqnE"
                     /locus_tag="VFV87_03375"
     CDS             complement(3122..4252)
                     /gene="mqnE"
                     /locus_tag="VFV87_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX5102822.1"
                     /translation="MPSLDSIREKVLAGGRLSLEDGLFLYEPTTPLHEVGELANLVRE
                     RKNGNRAYYNINTHLNATNICVYRCTFCAFRSDLREPKGYVMNDEQILARGQEAVDAG
                     CTEMHIVGGLHHQKKYEWYVDLVRILHEAYPELHLKAWTGVEINWFEFLAKKSVREIL
                     EDLIDAGLGSMPGGGAEIFHPEVRSKICEHKSDAGKWLDIHRTAHQLGLKTNCTMLYG
                     HIENAFHRIDHLIRLRELQDETGGFQTFIPLAFHPENTGLAHIKKPSAIMDLRTIAIS
                     RLMLDNIPHIKAYWIMLGIGTAQTALAYGADDIDGTVRHELIYHDAGATTPELLTSGQ
                     LERLILEAGRDPIERDTLYRRIVRDPAQPAKWSVGEELLAGI"
     gene            4532..5113
                     /locus_tag="VFV87_03380"
     CDS             4532..5113
                     /locus_tag="VFV87_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102823.1"
                     /translation="MLTLWAAAVAAGFWGLWTYASTPGRAERAPATWPQDSQLARSPG
                     RWTLVVFIHPRCPCTSASLTELEKLQAHLLEDIDIHIVSIQPRIGAEDWPDTPLVRRA
                     AAIPGAIIGVDVDGHEARRFHARTSGETLLFDPHGRLAFHGGITASRGHEGDNPGSDA
                     ILALVAGQSSAAPLACPAFGCPLRSDRDLASSE"
     gene            5119..6531
                     /locus_tag="VFV87_03385"
     CDS             5119..6531
                     /locus_tag="VFV87_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5102824.1"
                     /translation="MTDTLNTIPQQKVHARAAEIFAERLDQVHVRTDRWFAWLMVAQY
                     AAGVGVAWIVSPRTWAGTTSQVHIHVVAATFLGAVIVSLPIALAWQMPGRRLTRHVIA
                     AGQMLMSALLIHLTGGRIETHFHVFGSLAFLAFYRDWKVLLTATAVVALDHFLRGEFW
                     PQSVFGTLVASPYRWIEHAGWVLFEVWFLRLACLQNVREMRRDALQRAEIEHARELTE
                     QEVAQRTADLARKNQELDEFTYVASHDLQEPVRKLISFSKLLEQDLGSELNEQAAKDV
                     RFIVDAAGRMRDLIQDLLALSRTGRNALRLESIALDECVDRALDALDMRVQESGAEIV
                     RDGLPTVTGDATLLTQLYQNLVGNALKFVEPGKRPCIRLTAARADGGWVLGVEDNGIG
                     IKPEYAERIFRPFQRLHNRGEYEGTGIGLAICKKTVERHAGRIWVQSQPGAGSKFQFF
                     LGNPTEHERCKTSAELASVL"
     gene            6495..6977
                     /locus_tag="VFV87_03390"
     CDS             6495..6977
                     /locus_tag="VFV87_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5102825.1"
                     /translation="MQDIRRTSKRVVILLAEDDPGDQELTRRALQHDVLRADLRVVEN
                     GEEALDYLYRRGRFAEPGTAPRPDLILLDLNMPKLGGREVLAELKRDPSLCRIPVVVL
                     TTSNQETDIVKSYDLGCNSYIQKPVDIEQFMDSVRKLENYWFEVVTLPPVAGELACSA
                     "
     gene            7017..8501
                     /locus_tag="VFV87_03395"
     CDS             7017..8501
                     /locus_tag="VFV87_03395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5102826.1"
                     /translation="MTSLANCTKRVLIVEDDEGDAVLVERALARSEFDTSRADSREAA
                     LEQLAAAPCAAVLFDLSLPDSFGLDGVDVLRERFPDLPIIVVTGLSDEQLALEALHRG
                     AQDYLVKGAWSPDLLVRSLRYSIHRQHVQSENRRLLAELERQARHDALTGLLNRRSLL
                     AELEREWSRSNRTGEPLSCVMLDLDYFKRINDNHGHTAGDSVLQTVAAAIRTECRQSD
                     LVGRYGGEEFCIVLPATSQELAMAWAERIRWRLANTPLQFGGEPVAVTASLGVAERTP
                     ATPKPQALVERADQALRLAKQLGRDRVVASDRLSTISTSTAAYLSDQTSATATAAELM
                     SPPEAVLCPTATLKEAAQHLLGTRTNALPVVSDQGHLLGIVGERELANAVSSPRDWTR
                     PVTDVMQRKPPCFGQWVSAQVIREFLARSGASQVVVIEGQRPLGLVSRFAVLRWLHWR
                     DSVEPRRDEFSDPNCLADDSIADLTVDEGANALRQTEVFSMSGL"
     gene            8591..9031
                     /locus_tag="VFV87_03400"
     CDS             8591..9031
                     /locus_tag="VFV87_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5102827.1"
                     /translation="MNDEAVNRRDFHKLTAAAIGGLTAGALLGCRSEPAAGPPTAVSP
                     VATGDKHLCRGLNECKGQGKGGENACRGQGACATVAESSCGGNNECKGLGGCGDTVGA
                     NECKGQGGCHVPLMESAWDTLRKRKETEWAEKKLEFAGAPAKAG"
BASE COUNT         1612 a         2937 c         3074 g         1745 t
ORIGIN      
        1 gtcgcccgtg aggggggcgg gtttgagggc ttgttgggcg agcgtggcgt cgatgaattg
       61 gtcgatcgaa tctgcggacg ggttggaaac ccgtcctacg gcgtagtggc gggcgaagtc
      121 gtgccgtcgg tcccagtatt gggcgtagtc ggcgctagcc gcggcgcggc gcgcttggtt
      181 agtgcgaacg agattggcgc gggtttgctc ttcccagggc ttccaggcct cgtcggtcaa
      241 tggaaacgag gctgtcgcgg tgaggacgtg aaaatcatcc gtgccggcag gagcaatcgc
      301 gacagacgtt tcgcccaatt cgggacgccg cttcttgccg ccgacgaaaa tctccagccg
      361 gaaatgatgt ggaccgccgg ccagttcaat ctccgcgtcc ttctcttgat ctcccggctg
      421 cagcgggcgg atgcgggacg aaatcttgct ctcgaccggt tcccacgggt tgtgcccgtc
      481 ggttttgcca ttgggaaatg ggttctcaac ggccagcttg tcgtcgatat acagccgggc
      541 cgcgtgccgc gagcgaagca gcaggcggtg tttgccggcc ggcaggtcga tttgcgtatg
      601 cgcccagagg acgaacgggc tgctgcggtc gtcgatgacg ccgtgattgg tgtacttccg
      661 cgggacttcg acgaaggcca actccgcctg cgtgaacctc tcgctgggcg tcggtggaac
      721 gaaatcccag gtccacgcgt ccggcaggcc ttccagcact tcgacgagga cttcgtcctt
      781 gggaagcggc acattggtga catacggctt tggcagaatc gctttccagc gggcggcgat
      841 ccgctcggcg gacagtgctg tgcggtagat ggcgacttcg tcgatgccgc ccgcgaacga
      901 gttgccggga ttgttcttcg aagccgagcc gatccagacc tggtcgtcat cgacgaccgg
      961 cgcttcgtcc gatttgccgc cgtagtccca tgttcccttc gactcgcggc catcgacgta
     1021 accgcggact gagtcgcctt tgccgaacgt gtaactcacc gccacgtagt gccagccgct
     1081 acccgcggcg aatccggagt cgctcgtcca gcggtgccag tcgtcgctgt tgcccttgcg
     1141 attctccgcg cctcggaaca ggaacgaaat ccggcacgag cccccctgcc ctgccagccg
     1201 caaggcccag ttctgattgt cggcggcaaa gccggtgttg ccggtgcggc ctttgccgac
     1261 cacatagatt tgctggccgc cctcgagctt ggcggggctg acccaggctt cgagcgtgat
     1321 cgtgtcgccc tgggcaaaat cgagcgggct gtccacgccc ggatcgtcat atcgcagcga
     1381 tgccggagcg gagaacaaca ccgcctgatt gtgtcccgca aacagcggga actttgcggc
     1441 cggtgatccc ggctgagcga gtttcgccgg cccttccaca ccggccgcct gccacggctg
     1501 accgttcagt tcgcccgcgg gctggccgtc gtcgaatcgc caccacgcca ccggctgatc
     1561 ggcgcggaca atttcggcat acggcgtcgc cagccggtct ttgtcgtcgt ccgtgtcatt
     1621 ttgcgccgtg cggctcgcct gaccccaggc actggccgca agagcggccc agatcagcgg
     1681 tgcggcgatc aggaatcgca gcggtggccc aagccttgca cagaaagcgt ttccaggttt
     1741 catacttcga gccgcaaggg agacgcgggc caatcaggcg ggaattcctt gaggttaacc
     1801 cttcgacgtt gggacttcca gcccgaggcg cggtccgcca atccggatgc ggcgtgcgag
     1861 ttcactgttg aagacttccg tcagaactat aaaagaatta ataccctcac cgtttgacaa
     1921 gccaatcagc ggcaccttat cataacaagg cttgggacca ttgatgcgca acaaggtcgt
     1981 tgcgaccaag gaagcaggaa ggtcgctggt gaactgggac gtgcgtttgc ggccgcaagc
     2041 aacatgattg ggaaccgtcg tgatgaataa gaaagagtcc aagcccttca agaacctcct
     2101 tctgggcctg cgctcgcgct tgcggggcga cgtgaacgga ctggcggatg cggctctccg
     2161 caagacccgc gccgaatcgt ccggcgacct gtcgagcatg ccgattcaca tggcggacct
     2221 ggggaccgac gccttcgagc aggaattcaa cctcagcctg attgagcacg acggctccgc
     2281 gctggagcag atcgaagctg ctcttgagcg gatcgaagac ggcgtttatg gtgtctgcac
     2341 cgagtgcgag ggcaagattc ccaaggcccg gctggaggtg ctcccgcaca cgccccactg
     2401 tgtcaagtgc gccgaaaaag tcaccgcccg gtaaggattc aggggtcagg ggtcagggtt
     2461 caggagtcgg caggcagaag gatgtcgagc ggttgcctgt gccgcggaat cggtatgtga
     2521 cctatctggc gatcgcgctc gctggctttg cagcggattt ggcgacgaaa gaagcgacgt
     2581 ttcgctggct gggggtccat ccgcatttcg ttgccgccga cgatccagcc gcgattgcca
     2641 ggtggcgcgg cgatcctggg ctcaaccacc tgtggtggct gtgggaaggg cggctgggga
     2701 ttcagaccag cctcaacgaa ggcgcgctgt ttggcatggg cgcgggatac tggtggctgt
     2761 ttgccacgct gtcgatcgtg gcgctggccg gcgtcagcac gtggctcttc gccttcaaag
     2821 ctgccctcga ccgctggatc aacgtggccc tggcgtttgt aacggccggc atcctcggca
     2881 acttgtatga ccggctggga ctttttgact cgaccggact tgacgcgaaa ttccaccatg
     2941 ccgtccgcga ttggattctg ttcgtctggc ccgaaagcgg catgaaaatg ttcaatccct
     3001 ggcccaactt caacatcgcc gacagcctgc tggtgaccgg agccatcatg ctggtcgtcc
     3061 acgcggtcgt gtggcgggag aagaaggaag atgccagtga caagagcgag cggactgccg
     3121 cttagattcc cgccagcaat tcttcgccaa cactccactt cgctggctgg gcggggtcgc
     3181 ggacgatgcg gcggtagagg gtatcgcgct cgatggggtc gcggccggct tcaaggatga
     3241 gccgttcgag ttggccggac gtgagcaact cgggcgtggt cgcgccggca tcgtgataga
     3301 tcagttcgtg gcgcacagtg ccgtcgatgt cgtcggcgcc gtaggccagg gccgtctggg
     3361 ccgtgccgat gccgagcatg atccagtacg ccttgatgtg cgggatgttg tcgagcatca
     3421 agcggctgat ggcgatcgtt cgcaagtcca tgatggccga gggctttttg atgtgcgcca
     3481 gaccggtgtt ttccggatgg aaggccagcg ggatgaacgt ctggaagccg cccgtttcat
     3541 cctgcaattc gcggagccga atcaaatggt cgatgcggtg gaaggcgttt tcgatgtggc
     3601 catagagcat cgtgcagttg gtcttgaggc cgagctgatg agccgtgcgg tggatgtcga
     3661 gccacttgcc ggcgtcggac ttgtgctcgc agattttgct gcggacttcg ggatggaaga
     3721 tttccgcccc gccgccaggc atgctgccga ggccggcgtc gattaggtct tccaggattt
     3781 cgcggacgct cttcttggcc aggaactcga accagttgat ctcgacaccg gtccaggcct
     3841 tcaggtgcag ttccggatag gcctcgtgca ggatgcggac gaggtctacg taccactcgt
     3901 atttcttctg gtggtgcagg ccgccgacga tgtgcatctc ggtgcaaccg gcgtccaccg
     3961 cctcctggcc gcgggcgagg atctgctcgt cattcatcac atagccctta ggctcgcgga
     4021 gatcggagcg gaacgcacag aacgtgcagc gatacacgca gatgttggtc gcgttgaggt
     4081 gcgtgttgat gttgtagtag gcccggttgc cgttcttccg ctcgcggacg aggttcgcca
     4141 gttcccccac ctcgtgcagc ggcgtcgtcg gctcatacag gaacaggccg tcttccaggc
     4201 tcaggcgccc gccggcgagg accttttctc ggatggagtc gagggagggc acgagggttt
     4261 gggcttgtgg gtcgggctaa gaagtgctgg cctatcgatt caaccgctga gtctaggcgg
     4321 cacttaacgg cgtgtcgatg cggtcgctac aagtggcaca atccctcccc gattccagcc
     4381 gcatttggtg tgacctttcc aggcgacggg gctcggctgc aacctaggat ttcccgatcc
     4441 gtgcccctcc ttccagatcg ggtttcctgt gccgcccgtc gggcttccag ccgtcaattc
     4501 tcgtccttcc gcttcgcggt gggccgtccc catgctgacc ctttgggcgg ccgcggtggc
     4561 cgccggtttt tgggggctgt ggacctacgc ctccacgccg ggcagggccg agcgagcgcc
     4621 ggccacctgg ccgcaagata gccaactggc ccgttcgccc ggtcgttgga cgctggtcgt
     4681 tttcatccat ccgcgctgcc cttgcacgag tgcgtcgctg acggaactgg aaaaactcca
     4741 agcgcatctg ctggaggata tcgacattca cattgtttcc atccagccca ggatcggcgc
     4801 cgaagactgg cccgatacgc cgctggtccg tcgcgcggcc gcgattcccg gcgcgatcat
     4861 tggcgtcgat gtggacggcc acgaagcgcg gcgatttcac gcccgtacct cgggcgaaac
     4921 gctcctgttt gacccgcacg gacgcctggc ctttcacggc ggcatcaccg cgtcgcgcgg
     4981 gcatgaaggc gacaatcccg gcagcgacgc gatcctcgcg ctcgtcgccg gacaaagctc
     5041 cgcagcaccc ctggcctgtc ccgctttcgg atgcccgctc cggagcgatc gcgatttagc
     5101 gtccagcgag tgatctcgat gaccgacacc ctgaacacaa tcccgcaaca gaaggtccac
     5161 gcccgcgcgg cggaaatctt cgccgaacgc ctcgatcagg tccacgtgcg taccgatcgc
     5221 tggttcgcct ggctgatggt ggcgcaatat gccgcgggcg tcggtgtggc ctggatcgtc
     5281 tcgccacgta catgggccgg cacgactagc caagtccaca tccacgtggt tgccgctaca
     5341 tttctgggcg cggtcatcgt cagtctgccg atcgcactgg cgtggcagat gcccggccgc
     5401 cgcctcactc ggcacgtgat cgccgccggt caaatgctga tgtccgcctt gctgatccac
     5461 ctcaccggcg gccggatcga aacgcacttc cacgtctttg gctccctggc gtttctggcc
     5521 ttttatcgcg attggaaggt cctgctgacc gccacagcgg ttgtcgcgct cgaccatttc
     5581 ctgcgcggcg aattctggcc gcaatccgtc ttcggcacgc tcgtcgccag tccgtatcgc
     5641 tggatcgagc atgcgggctg ggtgctgttt gaggtctggt tcctgcggct ggcgtgcctg
     5701 caaaacgtgc gcgaaatgcg gcgcgacgcc ctgcagcgag ctgaaataga gcacgcccgc
     5761 gagctgaccg aacaagaggt cgcccagcgg acggccgatc tggcccgcaa gaaccaggaa
     5821 ctggatgagt ttacgtacgt cgccagccac gacttgcaag agcccgtccg caagctcatc
     5881 tcgttcagca agctgctgga gcaggatctc ggcagcgaac tcaacgagca agctgcgaag
     5941 gacgtgcggt tcatcgtcga tgccgcggga cggatgcgcg atctcatcca ggacctgctc
     6001 gccctctcgc gcactggccg caacgccctg cggctggaat cgatcgcgct ggacgagtgc
     6061 gtcgatcggg ccctcgacgc gctcgacatg cgcgtgcagg aatcaggcgc cgaaatcgtc
     6121 cgcgacgggc tgccgaccgt caccggcgat gccacgctgc tgacccagct ctaccagaac
     6181 ctggtgggca acgccctcaa gttcgtcgag ccgggcaagc ggccctgcat ccgcctgacg
     6241 gccgcgcggg ccgacggcgg ctgggtgctc ggcgtcgaag acaacggcat cggcatcaag
     6301 cccgaatatg ccgagcggat tttccgtccc ttccagcggc tgcacaaccg cggagagtat
     6361 gaagggaccg gcattgggct ggccatctgc aagaaaacgg tcgaacggca tgcggggcgg
     6421 atctgggtcc agtcgcagcc gggcgcaggc tcgaaattcc aattctttct gggcaatccc
     6481 acggagcacg aacgatgcaa gacatccgcc gaactagcaa gcgtgttgta atcctgttgg
     6541 ctgaagacga tccgggcgat caggagctga cgcgccgcgc gctgcagcac gatgtgctgc
     6601 gggccgacct gcgggttgtc gagaacggcg aagaggccct cgactatcta tatcgtcgcg
     6661 ggcggtttgc cgaaccgggg accgcgccgc gtcccgacct gatcctgctc gatctgaaca
     6721 tgccgaagct aggcggccgc gaggtgttgg ccgagctgaa gcgcgatccg tcgctctgcc
     6781 gcatccccgt cgtcgtcctg acgacttcca accaggaaac cgacatcgtc aagagttacg
     6841 acctgggctg caactcgtac atccagaagc cggtcgatat cgaacagttc atggactcgg
     6901 tgcggaagct cgagaactat tggttcgagg ttgttacgct cccgccggtg gccggtgagc
     6961 tggcctgctc cgcttgaatt cgctccactc cttcctcgca tccgtgagac cgaacgatga
     7021 catcccttgc caattgcacc aaacgcgtct tgatcgtaga agacgacgaa ggcgatgccg
     7081 tgctcgtcga gcgggctttg gcccgcagcg aatttgacac gtcccgggcc gacagccgcg
     7141 aagcggccct cgagcaactt gccgcagcgc cgtgcgcggc cgtgctgttc gatctttcgc
     7201 tgcccgacag cttcgggctg gatggagtgg acgtgctgcg ggagcgtttt cccgacctgc
     7261 cgatcattgt tgtaacgggg ctgtcggacg agcagctcgc gctggaagct ttgcaccgcg
     7321 gcgcccagga ctatctcgtc aagggcgcct ggagccccga cctcctcgtg cgctcgctgc
     7381 ggtattcgat ccaccggcag cacgtgcagt cggaaaatcg ccgcctgctg gccgaattgg
     7441 agcggcaggc ccggcatgac gccttgacgg gccttttgaa tcgccggtcg ctcctggcgg
     7501 agttggaacg cgaatggagc cgctcgaacc gcaccggcga gccgctctcg tgcgtgatgc
     7561 tcgatttgga ctacttcaag cggatcaacg acaaccacgg ccacacggcg ggcgacagcg
     7621 tgctccaaac ggtagccgcc gcgatccgca ccgaatgccg gcagtcggac ctcgtcggcc
     7681 gctacggcgg cgaggaattc tgcatcgtcc tgcccgcgac ctcgcaggaa ttggcgatgg
     7741 cgtgggccga gcgcattcgc tggcggctgg ccaatacgcc gctgcaattc ggcggcgagc
     7801 cggtggcggt gaccgccagc ctcggcgtag ccgagcggac gcctgcaact cccaagccac
     7861 aggcactggt cgagcgagct gaccaggcgt tgcgactggc caagcaactg ggccgcgacc
     7921 gcgttgtcgc gtccgaccgc ctcagcacga tcagcacttc aaccgcggcg tatttgagcg
     7981 atcagacttc ggccactgcg accgcggccg agttgatgag cccgccggaa gcggtcttgt
     8041 gcccgacagc gacgctgaag gaagctgccc agcacctgct cggcacgcgc acgaacgcgc
     8101 tgcccgtggt gagcgaccaa ggccacttgc tgggaatcgt cggcgagcgc gagctggcga
     8161 acgcggtttc gtcgccccgc gactggacac ggcccgtcac agacgtcatg cagcgcaagc
     8221 cgccgtgttt tggccagtgg gtcagcgccc aggtgatccg cgaattcctg gcgcgcagtg
     8281 gggccagcca ggtcgtcgtg atcgagggtc agcggcccct cggcctggtg agccgcttcg
     8341 cggtcctccg gtggctgcac tggcgcgaca gcgtcgagcc gcggcgcgac gaattctcgg
     8401 acccaaattg tctggccgac gattccatcg ccgatctgac ggttgacgag ggcgcgaacg
     8461 ctctgcggca gacggaagtg ttttccatgt ccgggctttg atcgtccgcg ttcgtgctgg
     8521 ccgccgcggg gctgcctcgc tagactaact tcgagccacg actattggac tagcgaggga
     8581 ggttgccagg atgaacgacg aagccgtcaa tcggcgggat ttccacaagc tcaccgctgc
     8641 tgccatcggc ggcctgacgg caggagccct gctcggctgc cgcagcgaac cggctgcggg
     8701 tccgccaacc gcggtttcgc cggtcgccac gggcgacaag cacttgtgcc gcggattgaa
     8761 cgagtgcaag ggccaaggca aagggggcga aaacgcctgc cgcggccaag gggcctgtgc
     8821 caccgttgcc gaaagctcgt gcggtgggaa taacgagtgc aaaggcctcg gcggctgcgg
     8881 cgacacggtc ggcgccaatg agtgcaaggg ccagggcggc tgccatgtcc cgctgatgga
     8941 atcggcctgg gacacactgc gcaagcgcaa agaaaccgag tgggccgaaa agaagctgga
     9001 gttcgcgggc gcgccggcga aggcgggata gggccaatgc ggagtgcgga acgcggagtg
     9061 cggaatgcgg agtggacatg agccgcatac acctccgtcc caggctctgc ctgggactga
     9121 acattcccga ggctctgcct cgcagcaggg cgggcgaggc ggcagcaccg cttagctctg
     9181 gcatgcgtcg gcgatgccta tcgcgatgca cgatgccaac aggcataatg cgagggcatg
     9241 accaccgcgc gccatccgcc agcccccgtc cgctcgggca atcgaccgca atacacgctc
     9301 cggcgactgt tcctctgggt gttcgtcctg ggcatcgcgc tgggcattgg ccgctggacc
     9361 tatgtctg
//
LOCUS       DASUGB010000174         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1270118, whole genome shotgun sequence.
ACCESSION   DASUGB010000174 DASUGB010000000
VERSION     DASUGB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270118"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFV87_03405"
     CDS             <1..462
                     /locus_tag="VFV87_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HEX5102828.1"
                     /translation="AGNSREQVRELLTAEVQTIIYRLTRIGGANFFGTPNIQVKWLGG
                     AMTVTKPQHLSSYEDLDAFIQLSADLFVAALNSRTTGITFVSESVKIAEPDEGESQCQ
                     VCGEPLTTDLVFCSACKTPHHRECWEYFGGCSTYACGNKKYLVRSKGKAKK"
     gene            510..1889
                     /locus_tag="VFV87_03410"
     CDS             510..1889
                     /locus_tag="VFV87_03410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HEX5102829.1"
                     /translation="MTHTLDLDDTIVAIASAPGGALRGIVRVSGPDVLRIVGDVFTAD
                     DPQSLMNATRPSLARGQIKLPRDLPPCPALVYLWPNERSYTRQPSAELHLPGSPPLLE
                     AALEAVCQAGARLARPGEFTLRAFLAGRLDLTQAEAVLGVIDARGQAELNRALAQLAG
                     GLAQPLHALRDRLLDLLAHLEAGLDFVDEDIEFISSSELEGQLAAAAGEVAALARQMT
                     SRGEAGKLPRVILVGRPNAGKSSLFNALAGGDAAIVSPVAGTTRDYVTCRVQQDGCDF
                     LLVDTAGDTAEASDPIAAAAQAAMREQAEQADLILVCVDASRPMAEWEGDPMVRKPAL
                     FVWTKCDVAAPGGMRTDQEVHPTGITTSSRTGQGIAELRRAIAMALQAAPAEASAIAG
                     TAERCRDSLRVAFESLQRAQEAAGVGLGEELVAAEVRMALDALGQVVGAVYTDDVLDR
                     VFSRFCIGK"
     gene            complement(1939..>2459)
                     /locus_tag="VFV87_03415"
     CDS             complement(1939..>2459)
                     /locus_tag="VFV87_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102830.1"
                     /translation="KQFWPGYSQPVECFLFRFQYVLTVEDDERSFSSIGIAGPLAHAF
                     VADLGDLPPDDIYAAFAGWQAAHQDIKEFDVARLSKSEKLEVERLKRRLHDAGYREIQ
                     PQSMGYFFGAKALVALCQREDVPGIAVADFQDISFLPQRSTRRPLGPAEAYCIYKGRK
                     LLRAFNRQGGGE"
BASE COUNT          432 a          800 c          809 g          418 t
ORIGIN      
        1 gccggcaaca gcagggaaca ggtccgcgag ctgctgaccg ccgaagtgca gacgatcatt
       61 taccgcctga cgcgcatcgg cggggcgaac ttcttcggca cgcccaacat ccaggtcaaa
      121 tggcttggcg gcgcgatgac cgtcaccaaa ccgcagcacc tgtcgagcta cgaagacctg
      181 gacgccttca ttcagctatc ggccgacctg ttcgtcgcgg cgctcaactc gcgaaccacc
      241 ggcatcacgt tcgtttccga gagcgtcaag atcgccgagc ccgacgaagg cgaatcccaa
      301 tgccaggtct gcggcgagcc gctcactacc gatctcgtct tctgctccgc ctgcaaaacg
      361 ccccaccacc gcgaatgctg ggaatacttc ggaggctgct caacctatgc gtgcggcaat
      421 aagaaatacc tcgtccgctc caaaggcaaa gcaaagaaat agccgccctt agccgggacc
      481 agttgcgtcg aatcggctcc gtttcaacca tgacccacac cctcgacctt gacgacacga
      541 ttgtcgcgat tgcttcggcg ccgggcggcg cgctgcgcgg catcgtccgc gtgagcgggc
      601 cggatgtgct gcggatcgtg ggggacgtgt ttaccgcaga tgatccgcaa tcgctaatga
      661 acgctactcg cccttcgctc gcgcgaggac aaatcaagct tccgcgcgac ctgcccccct
      721 gccctgccct ggtctatctg tggccgaacg aacgaagcta tacccgccaa ccctccgcgg
      781 agctgcacct gccgggctca cccccgctat tggaagctgc tttggaagca gtctgccagg
      841 ccggcgcgag gctggcgcgg ccgggggagt ttacattgcg ggcctttctg gccgggcggc
      901 tcgacctgac gcaagccgag gcggtgctag gcgtgatcga cgcccgcggg caggcggaat
      961 tgaaccgggc gctcgcgcag ctcgccggcg gcctcgctca gccgctgcat gcgctgcgcg
     1021 accggttgct cgatctgctg gcgcatctgg aggcggggct ggatttcgtg gacgaggaca
     1081 tcgagttcat cagctccagc gagttggagg gccagcttgc cgctgccgcg ggcgaagtcg
     1141 ccgcactggc ccgccagatg accagccgcg gcgaagccgg caagctgccg cgagtgatcc
     1201 tggttggccg gccgaacgcc ggcaagagca gcctcttcaa cgcgctggcc ggcggcgatg
     1261 cggccatcgt ctcgccggtc gctggcacca cgcgcgatta tgtgacctgc cgggttcagc
     1321 aggacggctg cgactttctg cttgtcgata cggcgggcga tacggcggag gcgagtgatc
     1381 cgattgccgc cgcggcacag gccgcgatgc gcgagcaagc cgaacaggcg gacctgatcc
     1441 tcgtctgcgt cgatgcttcg cggccgatgg ccgagtggga gggtgatccg atggtcagga
     1501 agcccgcgct tttcgtgtgg accaagtgcg atgtggccgc gccgggcggg atgcggacgg
     1561 accaggaggt ccatcctacg gggatcacga cgagcagccg cacgggccag ggaatcgccg
     1621 agctgcgccg cgcaattgcg atggcgctcc aggccgcgcc cgcggaggcc agcgcgattg
     1681 ccggcacggc cgagcgctgc cgcgatagcc tgcgagtggc gtttgagtcg ctccagcggg
     1741 cacaagaggc tgcaggcgtc gggctgggcg aggaactggt cgccgccgaa gtccgcatgg
     1801 ctctcgacgc gctcggtcag gtcgtcggcg cagtctatac cgacgacgtg ctcgatcgcg
     1861 tctttagccg gttttgtatt gggaagtaga gtgggtgagt ggtgagtggt tgtcacagat
     1921 gcaccactca ccactcacct actcaccacc cccctgccga ttaaacgccc gcagcagctt
     1981 ccgccccttg tagatgcaat acgcttcggc agggccgagg ggccggcggg tgctgcgctg
     2041 cgggaggaag ctgatgtctt gaaaatcggc gaccgcgatg ccggggacat cttcacgctg
     2101 gcaaagtgcg acgagggcct tggcgccgaa gaaatagccc atcgactgcg gctggatttc
     2161 gcgatagccc gcgtcgtgca gccgccgctt gagccgttcc acttcgagtt tttcgctctt
     2221 actcaggcgg gccacgtcga attccttgat gtcctgatgc gccgcctgcc agccggcgaa
     2281 cgcggcgtag atgtcgtccg gcggcagatc tcccaggtcc gcgacaaacg catgcgccag
     2341 cggcccggcg atgccgatgc tgctgaacga gcgctcgtcg tcttccacag tgaggacgta
     2401 ctgaaaacgg aatagaaagc attcgaccgg ctggctatag ccgggccaga actgcttcc
//
LOCUS       DASUGB010000175         5146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_575969, whole genome shotgun sequence.
ACCESSION   DASUGB010000175 DASUGB010000000
VERSION     DASUGB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575969"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFV87_03420"
     CDS             <1..706
                     /locus_tag="VFV87_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5102831.1"
                     /translation="VGTGINTHPEFGRRVAAALAKEAGLAFVEAKDHFEANAQRDGLV
                     ECHGQLRTIAVTLFNVSNNIRWLGSGPRCGFYEVKLPDRQPGSSIMPGKVNPVMCESL
                     MQVAARVIGNDQTLAFSGATGGQFQLNIMMPVMGHAALESVTLLASSSRAFVDFCVSE
                     MEANPEACEASVEKSLSMVTSLNPHIGYEKAAAIAKKAFATNKTIREICREEGILPEP
                     TLREALDPMSMTEPQE"
     gene            complement(868..1635)
                     /locus_tag="VFV87_03425"
     CDS             complement(868..1635)
                     /locus_tag="VFV87_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5102832.1"
                     /translation="MLHDLIALVALTAMEIVLGIDNIVFISVVSSRLPVHQQPLARRV
                     GLLAAMGTRIVLLLAISLIVKADDPFFNLTSIPLPEVLNAWLAEHKAINGISVRDLIV
                     LGGGLFLIWSSVREIHHKVEGEHEEHSTPEAVTFRGVISQIAVLDIVFSLDSVITAVG
                     MAESIWVMITAVVLAVGVMMLFAGPVSAFVEKHPTVKMLALSFLLLIGVMLVADGLGT
                     HIDKGYIYFAMAFALLVEMLNLRMKSKARQRRQKAAH"
     gene            complement(1668..2504)
                     /locus_tag="VFV87_03430"
     CDS             complement(1668..2504)
                     /locus_tag="VFV87_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102833.1"
                     /translation="MRITVPSSVAAVGLLVLSFLSPGCGRPTLKSSGGYVLTYRLAQG
                     ETADGQDLARAMQHRLQAGDFPHASVRHTAGDALVVELPGANAPQVERAQKLLQNAGH
                     LAFRIVADKQLDQKTAEAALAGNGLDAEADAAFAWVKCNAEALQGEDWMVTRDNPAGG
                     QEILVLVGPDDVTGNELAKAVVARDSAARPCIQGSLRPIGAQKMSLLTGANIDRRLGI
                     ILDGELLTWPVIRSPIGQDLQITGNFTEDEVQRIVTILKSGTFPGRLNDEPESVQEVQ
                     PP"
     gene            complement(2501..4204)
                     /locus_tag="VFV87_03435"
     CDS             complement(2501..4204)
                     /locus_tag="VFV87_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5102834.1"
                     /translation="MIKVRSIPVIYRNVKRWTEIISVLSKYGLADWLAHSNIDFIKDR
                     LASKDGEAIARLTREARIRLTLTELGPTFIKLGQLLSTRPDVVGKELALELQKLQAST
                     PADPPEAVQKIVETELGEPLDELFAEFDLTPIASASIGQVHKARLKTGERVVVKVQHK
                     GIESTVNDDLDVLSGLAQLAEQISEFKPYRPMANMAEMGRTLRRELDFGREERNLHQF
                     AAIFKDDPTICIPRAYTELCTSRVLTMDMIEGIPVSQPGLLEAAGLDRAEVARRGAEI
                     YMQMIFTHGYFHADPHPGNIMLLPGNVIGLLDFGMVGRIDDRLRENIEDMLLAIVQHD
                     VPLLVRIIKQIGQPPPQLDESGLSNDVADFVGHYSTQSLEFFDVGAALIDMTDIMRRH
                     GIMLPPQVATLIKTLITLEGTSKLLSPRFSLMEVMQPFQKGMLLRRLSPVRQAKKLRR
                     LYMEVEQLVEVLPQKVIQILEQIQSGKFDVHLDHRGLGPSVNRLVLGMLASALFLGSS
                     LLLANKVPPLIFNQPEPWYLGLHQISILGLMGCSVSLLVGLRLLWAIGKSGHLDQKVS
                     K"
     gene            4377..>5146
                     /locus_tag="VFV87_03440"
     CDS             4377..>5146
                     /locus_tag="VFV87_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX5102835.1"
                     /translation="MRIWRGSPYPLGATWDGAGVNFGVFSESATKVELCLFDSPESTA
                     ESERIELPERTYQIWHGYFPDLRPGQLYGIRVHGPYEPREGLRFNPNKLLLDPYAKAI
                     GRDMKWDNRLFGYEIGNPQGDLSLDGSDSAAFAPLGTVVDTAFTWGNDLPLRIPWEKS
                     ILYEAHVKGFTKRMPGVPEELRGTYAGLASEAAVAHLVELGITAIELLPVQYHSDERF
                     LVDHGRVNYWGYNTLGFFAPDPRYCATTDPDRKLHEFKS"
BASE COUNT          972 a         1665 c         1540 g          969 t
ORIGIN      
        1 ggtcggcacc ggcatcaata cgcacccgga gttcggccgt cgcgtcgccg cggcactggc
       61 gaaagaggcc ggcctcgcgt tcgtcgaggc gaaagatcat ttcgaagcca atgcccagcg
      121 cgacgggctg gtcgaatgtc acggacagct ccgcaccatc gccgtcacgc tcttcaatgt
      181 gtcgaacaac atccgctggc tcggcagcgg cccgcggtgc ggtttctacg aagtcaagct
      241 tcccgaccgc cagccgggca gctccatcat gcccggcaaa gtgaatccgg tgatgtgcga
      301 gagcctgatg caggtggcgg cccgggtgat cggcaacgac cagacgctgg ccttcagcgg
      361 cgcgaccggc ggccagttcc agctcaacat catgatgccc gtcatgggac acgccgccct
      421 ggagagtgtg acgttgctcg cttcctcctc gcgtgccttc gtagatttct gtgtcagcga
      481 gatggaagcc aatcccgaag cctgcgaagc ctcggtcgag aagagcctct cgatggtgac
      541 cagcctcaat ccgcacatcg gctacgaaaa agcagcggcc attgccaaga aggccttcgc
      601 gaccaacaaa acgattcgcg aaatctgtcg cgaggaaggc atcctccccg agcccacgct
      661 ccgcgaagct ctcgacccga tgagcatgac tgaacctcag gagtagcgca gcacggctct
      721 cctgtcccag ttctctccgc aaccacctcc acttcgcccg caacggcgga ttccaacgat
      781 tcagtcaccg ggcccgccac gcactacgga tcaatttcgc ccgcaacgtg acaatccgtt
      841 ggctccctgg aaagaccgct agatcggtca gtgcgcagcc ttctggcgcc tttgcctggc
      901 cttggacttc attcgcagat tgagcatttc caccagcagg gcgaaggcca tggcgaagta
      961 gatgtagccc ttgtcgatgt gcgttcccag accgtcggcc accagcatga cgccgatcaa
     1021 cagcaggaag ctgagagcga gcatcttcac ggtcgggtgc ttctcgacga aagccgagac
     1081 ggggccggcg aagagcatca tgacgccgac ggccaggacg acggcggtga tcatgaccca
     1141 gatcgattcc gccatgccga cggccgtgat caccgaatcg agcgagaaca cgatgtcgag
     1201 cacggcgatc tgagaaatga cgccgcgaaa ggtcacggcc tctggggtgc tgtgttcttc
     1261 gtgttcccct tccaccttgt gatggatttc acggacgctg gaccagatta gaaacaaccc
     1321 gccgcccagc acaatcagat cgcggaccga aattccgtta atggccttgt gctcggccag
     1381 ccacgcgttc agcacctccg gcagcgggat cgacgtcagg ttgaagaatg ggtcgtccgc
     1441 cttgacgatc agcgatatcg ccaacagcag cacgattcgc gtccccatcg cggccaacag
     1501 gcccacccgc cgcgccagcg gctgttgatg caccggcagc cggctactga cgacggagat
     1561 gaagacgatg ttgtcgatcc ccaggacgat ttccatcgcc gtcagggcga ccagggcgat
     1621 caggtcgtgg agcatgcagt tgactggtcg gctgaggagg ctggtctcta gggtggctgg
     1681 acttcttgga ccgattccgg ctcgtcgttc aatcgcccgg gaaacgtgcc ggatttcagg
     1741 attgtaacaa tccgctgcac ttcgtcctca gtgaagttgc cggtgatctg caaatcctgt
     1801 ccgatcggtg accgaatcac gggccaggtc agcaattccc cgtccagaat gatccccagc
     1861 cggcgatcga tgttggcgcc tgtcagcagg ctcatcttct gagcaccgat gggacggagc
     1921 gaaccctgga tgcacggccg cgctgcggaa tcgcgcgcca caaccgcctt ggccaactcg
     1981 ttgccggtga cgtcatccgg acccacgagc accagaattt cctggccgcc ggcggggttg
     2041 tcccgcgtca ccatccaatc ctcgccttgg agcgcctcgg cattgcattt gacccaagca
     2101 aacgctgcgt ctgcctccgc atccagcccg ttgccggcga gcgccgcctc ggcagttttc
     2161 tggtcgagct gcttatcggc tacgatgcgg aacgctagat gcccggcatt ttgcagcagc
     2221 ttttgcgcgc gttccacctg cggggcgtta gcgcctggca attcaacgac caaggcgtcg
     2281 cccgcggtgt gccggactga ggcgtgcgga aagtctccgg cctgcagacg gtgctgcatg
     2341 gccctggcca aatcctgtcc gtcggccgtt tcgccctgcg caagtcgata ggtcaatacg
     2401 tatccaccgc tgcttttgag cgtcggacgg ccgcagcctg gactcaagaa actcaggacc
     2461 agcaaaccca cggctgccac gctgctggga acggtgattc tcatttcgac accttctgat
     2521 ccaaatgccc gctcttgccg atcgcccaca ggagccgcag cccgacgagc aggctgacgc
     2581 tgcagcccat gaggccgagg atcgaaatct ggtgcaggcc cagataccac ggctctggct
     2641 ggttaaatat cagcggcggg accttgttcg ccaagagcag cgaggagccc aaaaacagcg
     2701 cgctggccag cattcccagc accaggcggt tgacgctcgg tcccaggccg cggtggtcga
     2761 ggtggacgtc gaatttgccc gactggattt gctccagaat ctggatgacc ttttgcggca
     2821 gcacctcgac gagctgctcg acctccatat acagacgccg cagttttttc gcctgcctga
     2881 cgggcgacag ccggcggagc agcattcctt tctgaaacgg ctgcatcacc tccatcaggc
     2941 tgaaccgcgg gctgaggagc ttcgacgtgc cttcgagtgt gatcagcgtt ttgatcagcg
     3001 tggcaacctg cggcggcagc atgatgccgt gccgccgcat gatgtcggtc atgtcgatca
     3061 gggccgcgcc gacgtcgaaa aactccagcg attgcgtcga ataatgcccc acgaaatcgg
     3121 ccacgtcgtt ggacaggccc gattcgtcga gctgcggcgg cggctggccg atctgcttga
     3181 tgattcgcac caaaagcggc acgtcgtgct gcacgatcgc cagcagcata tcttcgatgt
     3241 tctcccgcag ccggtcgtcg atccggccga ccatgccgaa atccaagagg ccgatcacgt
     3301 tccccggcaa gagcatgatg ttgccggggt gcggatcggc atggaagtag ccgtgggtga
     3361 agatcatctg catgtagatc tctgccccgc ggcgggcgac ttccgcccgg tccagcccgg
     3421 ccgcctccaa aagccccggc tgggaaacgg gaatcccctc gatcatgtcc atcgtcagca
     3481 cgcgcgacgt acacagctcg gtataggccc gcggaataca gatcgtcggg tcgtccttga
     3541 aaatcgccgc gaattggtgc aggttccgct cttcgcggcc aaaatcgagt tcccgtcgca
     3601 gcgtccggcc catttccgcc atgttggcca tcgggcggta cggcttgaat tcggaaatct
     3661 gctcggcaag ctgcgcgagg ccgctgagca cgtcgaggtc atcgttgacc gtgctctcga
     3721 tccccttgtg ctggaccttc acgaccaccc gctcgcccgt cttcagccgg gccttgtgga
     3781 cctgcccgat cgacgccgat gcaatcggcg tcaggtcgaa ttccgcaaac agctcatcca
     3841 gcggctcgcc caattccgtc tcgacgattt tctgcaccgc ctccggcggg tcggcggggg
     3901 tcgaagcctg cagcttttgc agctccaggg ccaattcctt gcccacgacg tcgggccgcg
     3961 tgctgagcag ttgccccagc ttgataaacg tcggccccag ctccgtcagc gtcagccgga
     4021 tccgtgcttc ccgcgtcagc cgggcgatcg cctcgccatc cttcgaggcg agccggtcct
     4081 tgatgaagtc gatgttcgaa tgggccagcc agtcggccag gccgtacttg ctgaggaccg
     4141 aaatgatctc cgtccagcgc ttgacgttgc gataaatgac cggaatggag cggactttga
     4201 tcacaagtcg attgtacttc gcaggcatct cgctccgcga gacgcgatgg atactccctg
     4261 cgcaggggcc tcggcgcagg gaaagtcgct cgctacttgc ggagccagtg gcctacggct
     4321 gcgccgcaca tccgctatat tacctgcatc aactggagct tcttcgcgag ccctctatgc
     4381 gaatctggcg cggttctcct tatccgctcg gcgccacgtg ggacggcgcg ggcgtcaact
     4441 ttggcgtctt ttcggaatcg gccaccaagg tcgagctgtg cctcttcgac tcgcccgagt
     4501 ccacggccga gagcgagcgg atcgagctgc ccgagcggac ctatcaaatc tggcatggct
     4561 actttcccga tttgcgaccg gggcaacttt atggaattcg cgtacacggg ccctacgagc
     4621 cacgtgaagg gctgagattc aaccccaaca agcttttgct cgacccgtac gccaaggcga
     4681 tcggccggga catgaagtgg gacaaccggc tgttcggtta cgagatcggc aatcctcaag
     4741 gggatctttc gctggacggc agcgacagtg ccgcgtttgc gccgctgggg accgtggtcg
     4801 atacggcatt cacctggggc aacgatctgc cgctgcgcat tccgtgggag aagtcgatcc
     4861 tttatgaggc ccacgtcaag ggcttcacaa agcggatgcc cggcgtgccg gaagagctgc
     4921 gcgggaccta cgccggcctg gccagcgaag cggccgtcgc gcatctggtc gagctgggga
     4981 tcacggcgat cgagctattg ccggtccagt atcactccga cgagcggttc ctcgtcgatc
     5041 acggccgggt gaactactgg ggctacaaca cgctgggctt tttcgctccc gatccgcggt
     5101 actgcgccac gaccgacccc gaccgcaagc tgcatgagtt caagtc
//
LOCUS       DASUGB010000176         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1549314, whole genome shotgun sequence.
ACCESSION   DASUGB010000176 DASUGB010000000
VERSION     DASUGB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549314"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2323
                     /locus_tag="VFV87_03445"
     CDS             <1..2323
                     /locus_tag="VFV87_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5102836.1"
                     /translation="SLLHGEAAEAVAELMKSTAPNWYEQVAPLAAKDSALTRVLAESR
                     AASQERLKRELGAFLRELSHQQPLLLFIDDLHWADASTVDLLAYLGGKCAGMRTLLVL
                     TYRPTDLALSRHPWGSVKLDLQARGVCHELALEFLTRGDLDRYLELEFPEHCFPEEFA
                     TLVYVRTEGSPLFMADLLRYLRDRQVLTREQGRWRLAQSVPDLRRDLPESVRGMIQRK
                     IDQLDDDDRRLLVAAGVQGYEFDSAIVARVVQRDAADVEDRLDELDRVHAFVRLVREQ
                     EFPDRTLTLRYRFVHVLYQNTLEATLLPTRRASLSAAVSQALLSHYGEKSGEVAAELA
                     LLLEAARDFSHAADYFLAAAQNATRVFANQEAVVLARRGIEVLASLPDSPERARQELA
                     LQITLGPALFATRDWTAPDVEAAYTRAHTLCRLLGESPDQFPALWGLFLFHIARGQIQ
                     TSLGLGDQLLSLAEHARDPALLLQAHHAVGPTYALTGDWATAQPHLDQAIAHYDRRQH
                     DTHAFFYGGHDPCVCCLSFAAKSLWMLGYPEQALQRGREALTLAHELGHPTSLAHTQF
                     SVAMFHQFRRDVAETLKLAEALQRLAGDQGLVFYLAGGLVLKGWALVELGQRDEGIAL
                     IREGFETGGATRAHWRTYCLALLAEACARRGDLTDGLAALTEALTVIQKTRISMFEPE
                     IHRLKGQLLLHLDPENPAAAEACYNQAVAIARRQQAKSLELRATMGLARLWRRQGRHD
                     EGHAALAAVYDTYTEGFTTPDLVDAAAQLNGLA"
     gene            complement(2418..>3037)
                     /locus_tag="VFV87_03450"
     CDS             complement(2418..>3037)
                     /locus_tag="VFV87_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5102837.1"
                     /translation="WAIPLPENLHLADAGPLLCGGITVFSPLAMHAKPTDRVGIIGIG
                     GLGHMALKFAAAYGCDVTAFTSSERKFEEAKSFGAHHVVSSRDSDAMAKLAGSFDLLI
                     STVNVSLDWNALIGTLAPNGRLHVVGAVLEPIPVAAFPLIMGQRCVSGSPTGSPVAIG
                     AMLDFAARHTIVPQTEHFPMSQINEAFARLESGKARYRIVLDADF"
BASE COUNT          549 a          971 c          982 g          535 t
ORIGIN      
        1 gagcctgctt catggagaag cggccgaggc ggtggccgag ttgatgaagt ctaccgcccc
       61 aaactggtat gagcaggtcg cgcccctggc agcgaaagac tctgcactga cccgcgtcct
      121 tgctgagtcg agagccgcgt cacaggaacg cctgaaacgc gaactgggcg ccttcctgcg
      181 ggaattgtcg caccagcagc cgctgctgct gttcatcgac gatttgcact gggcggacgc
      241 ctcgacagtt gatcttctgg cgtacctggg cggcaaatgc gcgggaatgc gcaccttgct
      301 ggtgttgacc tatcgcccaa ccgacctggc attgagcagg cacccatggg gatcggtgaa
      361 gctggacctg caggcgcgcg gcgtttgcca cgagttggcg ttggaatttc tgacacgagg
      421 ggatctcgat cgctatcttg agctcgaatt ccccgagcat tgtttcccgg aggagttcgc
      481 gacactcgtc tacgtccgga cggaggggag cccgctgttc atggccgacc tgttgcggta
      541 tctgcgcgac cgccaggtgc tgacacggga gcagggacgc tggcggctgg cgcaatcggt
      601 cccggacctc aggcgcgatc tgccagagtc agtccgcggg atgatccagc gaaagattga
      661 tcagctcgac gacgacgacc gccgcctgct ggtggcagcc ggcgtgcagg gctatgagtt
      721 cgactccgcg atcgtcgccc gcgtggtgca acgtgacgcg gcagacgtgg aggacagact
      781 ggacgaactg gaccgcgtcc atgccttcgt acgtctggtg cgggaacagg agttccctga
      841 tcggacgctg acgctacggt accgcttcgt acacgtcctc tatcagaata ccctggaagc
      901 aacgctgctg ccgacaagac gcgcgtcgct cagcgcggcc gtgtcgcagg ctctgctgtc
      961 gcattacggc gagaagagtg gagaggtcgc cgcggaactg gcactgcttc tggaggcggc
     1021 ccgggacttc tcgcacgccg ccgactattt cctggcggct gctcagaatg cgacccgcgt
     1081 ctttgccaat caggaggccg ttgtgctcgc acgccggggg atcgaagtgc ttgcgtcgct
     1141 gcccgactct cccgagcgcg cccgtcagga acttgcgctg caaatcaccc tggggcctgc
     1201 gctgttcgcc accagggact ggacggcgcc ggacgtcgag gcagcctaca cgcgtgcgca
     1261 caccctgtgt cgattgctgg gagaatcacc ggaccagttc cccgcgctgt ggggactgtt
     1321 cctcttccac atcgcgcgcg ggcaaatcca gacaagtctc ggcctcggcg accagctcct
     1381 gagtctggca gaacacgcgc gggacccggc gctgctgctc caggcgcatc acgcggtcgg
     1441 accgacgtat gccctcaccg gcgactgggc caccgcacaa ccgcacctcg accaggcgat
     1501 tgcccattac gaccgccgcc agcacgacac ccatgccttt ttctatggcg gccatgaccc
     1561 atgcgtgtgc tgcctgagct tcgcggccaa gtctctctgg atgctcggtt atcccgaaca
     1621 ggctttgcag agggggcggg aggcgctcac gctggcccat gaactcggcc atcccacgag
     1681 tttggcgcat acgcaatttt cggtcgcgat gttccatcag tttcggcggg acgtggccga
     1741 aaccctcaag ctcgccgagg cgctgcagag actcgccggc gaccaggggc tcgtgttcta
     1801 tctggcggga ggattggtcc tgaagggctg ggccctggtt gaactggggc aacgcgatga
     1861 ggggatcgca ctgattcgtg agggatttga aaccgggggc gccacacgcg cgcattggcg
     1921 cacctattgc ctggccttat tggccgaggc ctgtgccagg cgaggagatc tcacggacgg
     1981 gctcgccgcg ctgacagaag cactcacggt gatccaaaag acccggatca gcatgtttga
     2041 gccggaaatc caccggctca aggggcaact cctgctgcat cttgatccgg agaacccggc
     2101 cgccgccgaa gcctgttaca accaggccgt cgcgatcgcg cgccgtcaac aggcgaagtc
     2161 gctggagctg cgagccacaa tgggactggc ccgcctgtgg cgacgacagg gccgccacga
     2221 cgagggtcac gcggcgctgg ccgcggtcta tgacacctat acggagggtt ttacgactcc
     2281 tgacctagtg gacgccgccg cgcaattgaa tggcctcgcc tgaccttcga tgcgctacat
     2341 gcacagggac cacgcggcga cccctcacca ggacgcgacc gcctgttcca aactcgaatt
     2401 catttccttc ggaaccgtca gaaatccgcg tccagcacga tgcggtagcg ggccttgccg
     2461 gattccagcc gcgcaaaggc ctcgttgatc tggctcatcg gaaagtgctc cgtctgcggc
     2521 acgatcgtgt gccgtgccgc gaaatcgagc atggctccga tcgccaccgg cgagcccgtg
     2581 ggagaaccgg aaacgcagcg ttgtcccatg atcagcggga atgccgcgac cggaatcggc
     2641 tccagtacgg cgccgacgac atgcaggcgg ccattcggcg cgagcgtgcc gatcagcgcg
     2701 ttccaatcga ggctgacgtt gaccgtgctg atcagcaggt caaagctgcc ggcgagtttc
     2761 gccatcgcat cggagtctct gctggagacg acgtggtgcg ccccgaacga tttggcctcc
     2821 tcaaacttgc gttcactgga cgtgaacgcg gtcacatcgc aaccgtacgc cgcggcgaac
     2881 ttcagggcca tgtggccgag cccgccgatg ccgatgatgc ccacacgatc ggtcggcttc
     2941 gcatgcatgg ccaacggtga aaagacggtg atcccgccac acagcagcgg tccggcgtcg
     3001 gccagatgca ggttttcggg cagcgggatg gcccagg
//
LOCUS       DASUGB010000177         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1966022, whole genome shotgun sequence.
ACCESSION   DASUGB010000177 DASUGB010000000
VERSION     DASUGB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966022"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFV87_03455"
     CDS             complement(<1..326)
                     /locus_tag="VFV87_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5102838.1"
                     /translation="MSFADTGIREQAGKQAHLPGAGGLIRIRGARTHNLKNVDLDIPR
                     GKLVVITGPSGSGKSSLAFDTIFAEGQRQYIESLSVYARQFVEQLERPDVDSIDGLAP
                     VICIDQR"
     gene            546..1655
                     /locus_tag="VFV87_03460"
     CDS             546..1655
                     /locus_tag="VFV87_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5102839.1"
                     /translation="MSRTAVFDASLGYFLQPIAGLLDDPAVTEIMVNGPDQIFAERAG
                     RLEAVPARFEDEDALLSAIHNLTQYVGRDIGPDQPILDARLPSGARVHAIIPPASRVG
                     PCLTIRKFRQDVLTLDDLVRLGSLSAEAREFLEICIRLRKNILVAGASGAGKTSLLGA
                     LTEAIPSDERILVIEDTAELRLRQPHVVALESRSASGGEGPSLRQLFIAALRMRPDRI
                     LVGEVRGGEALDMLQAMLSGHSGCLSTVHAATPRDALVRLETLSLMSDVELPVYVARS
                     QVASAVHVIVQIVRFPQDGTRRITRIAEVGPLDQNGQYQVFELFTTRVEGRAADGRLY
                     SHLEPGELRPTFADEVRQYGLEPLIRTTSKLWGSR"
     gene            1870..2079
                     /locus_tag="VFV87_03465"
     CDS             1870..2079
                     /locus_tag="VFV87_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102840.1"
                     /translation="MKLNSPLMRLLARIHRDERGGVSIETILIIAAIALPILIFLIKV
                     GWPRVKSFFDSGMTDLETGAGSASN"
BASE COUNT          361 a          719 c          709 g          477 t
ORIGIN      
        1 cgctggtcga tgcagatgac cggggccagg ccgtcgatcg agtccacatc tggccgctcg
       61 agctgctcga cgaactgccg ggcatagacc gacaggctct cgatatattg ccgctgcccc
      121 tcggcaaaga tggtatcgaa cgccagactg ctctttcccg acccgctcgg ccccgtaatc
      181 accaccagct tcccgcgcgg aatgtccaga tcgacattct tcagattgtg cgtccgcgcc
      241 ccgcggatgc gaatgagtcc gcccgcaccg ggaaggtgcg cctgtttccc ggcttgctct
      301 cgaattccgg tatccgcgaa gctcatccca tctgccgtcg gtgatcgatt gacgccaaag
      361 tctgtatcgt actccaactc acccccgccg cagccgcctg acagtggcaa cccttggcga
      421 cacttcattt ctggttttcg ttgacatccc catcgtggtc ggcttagtct caggtgttgg
      481 cagcgcgcgg cagaaatggc tcgaagatgt cggcttgctc tgcgctcacc cggacgcgat
      541 cgatcatgtc tcgtactgcg gtctttgacg ccagcttggg atacttcctg cagccgattg
      601 ccgggttgct ggacgacccg gcggtgacgg agatcatggt gaatggcccg gatcagattt
      661 tcgcggagcg ggccgggcgg ctggaggccg tgccggcgag gtttgaagat gaggatgcgc
      721 tcctttcggc gattcataac ctgacgcaat acgttggccg tgatatcggc cccgatcagc
      781 cgattctcga cgcccggctg ccgagcggcg cccgcgtcca tgcgatcatt ccgccggcgt
      841 cgcgcgtggg gccgtgcctg acgatccgca aattccgtca agacgtgctg acgcttgacg
      901 acctggtgcg gctgggttcg ctttcggccg aggcccgcga gttcctggag atttgcatcc
      961 ggctgcggaa gaacattctc gtcgccggcg ccagcggagc gggaaagacg tcgttgctcg
     1021 gcgctttgac ggaggcgatt cccagcgacg agcggattct ggtcatcgag gacaccgcag
     1081 agcttcgact tcgccagccg catgttgtgg ccctggagtc gcgttcggct tcgggtggcg
     1141 aggggccgtc gctaagacag ctcttcattg cggcgctacg gatgcggcca gaccggattc
     1201 tcgtcggcga agtccgcggc ggcgaggcac tcgacatgct gcaggcgatg ctcagcggcc
     1261 acagcggctg cctctcgacc gtccatgccg cgaccccccg cgatgccctc gtgcggctgg
     1321 agacgctctc gctgatgtcg gacgtcgagc tgccggtcta tgtggcccgt tcgcaggtcg
     1381 cctccgccgt ccacgtcatc gttcaaatcg tgcgttttcc gcaggatggc acgcggcgga
     1441 tcacccgaat cgccgaagtc ggcccgttgg accaaaacgg ccagtaccag gtttttgagc
     1501 tgtttaccac ccgtgtggag ggacgcgcgg ccgatggtcg gttatactcg catttggagc
     1561 cgggcgagtt gcgccccacc tttgctgacg aagtgcggca atatggcctg gagccgctga
     1621 ttcgcacgac aagtaagtta tggggttcgc gctaagcaca agacgagtca ggcgaagggc
     1681 gtacagccgt ttgcggcgcg gctccgcgac gattgactat tttttattga tttgcatcct
     1741 gctaccgctc gcggcgctgg tgctgcacgt cggcccgcga gcgatgcagt tggtctatga
     1801 ccttttgacc gtcgtggtcg cctggccgtt tttgtagcgg cagcggctcg acacgtgagg
     1861 agtagttccg tgaagctcaa ctctcccctg atgcgactgc tcgcgcgcat ccaccgggac
     1921 gagcggggcg gcgtgtcgat cgagacgatt ttgatcatcg ccgccatcgc cctgccgatc
     1981 ctgatcttcc tgatcaaggt gggctggccg cgcgtcaagt cgttcttcga ctcgggcatg
     2041 accgatctgg agaccggcgc cggcagcgcc tccaactagc gcaatgcttc caagtagcac
     2101 cgcacttccc acttacgccg tgcttgccag tctgctgctt ttcgggctcg tagccaccgc
     2161 ggcagtcacg gacctttcgc gccatcggat ctacaactgg acgacctatc ccggcatggt
     2221 cgccggcttg ctgctggctt tgggcggctg gatcttcgaa tcggca
//
LOCUS       DASUGB010000178         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_854561, whole genome shotgun sequence.
ACCESSION   DASUGB010000178 DASUGB010000000
VERSION     DASUGB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854561"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFV87_03470"
     CDS             <1..551
                     /locus_tag="VFV87_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5102841.1"
                     /translation="ERAIKRGTGELEGGEVEEIIYEGYGPGGVAVLCEIMTDKRSRTA
                     PEVRKIFDTHGGKLGATNCVAWMFERKGLLLVPAAKTTEEQLMEVALEAGAEDLKREG
                     DMFQIVCSPEAFTAVSDALTAAGIEPASKQLTRIPSNIVEVADVETARTVLALMEALD
                     DHDDVQNVSANFSIPESALAAL"
     gene            complement(577..999)
                     /locus_tag="VFV87_03475"
     CDS             complement(577..999)
                     /locus_tag="VFV87_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102842.1"
                     /translation="MQWRLHAVVALSVAALLTGCGSGIDTATVRGKVTLGGQPVPNVI
                     VNFQPENGRPAVGQTDASGNYELSTISSGDGAVPGMHKVSFSIKTEPPPSTAVAKQMS
                     GPVVAAPFNVKYRSGETSGLTREVKAGESNVFDFELEK"
     gene            complement(1030..1995)
                     /locus_tag="VFV87_03480"
     CDS             complement(1030..1995)
                     /locus_tag="VFV87_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102843.1"
                     /translation="MIAIIGILVSLLLPAVQAAREAGRRASCSNNMHQLAIAMHNYND
                     IYTALPICYSGDGTASETDWGKGWMVGILPFIEQDTLHKKIRWTPAPSGTSHTLGSPE
                     NQAVATVTIKSFVCISDGNNGRGAMSGRANVNPATIYGVTNYKACAGANWCTADPSTG
                     AITGQWSSAGAQVTEDVKAAPFPNNGNGLDAGNGIICRNGSNNPALWHELAFVTDGLS
                     NTFAVGEAVPAWCTHTTWWGWNHSTATCGIPLNYKPPAVLNGTQTMEENAADYLNNYS
                     FMSRHPNGAQFAMCDGAVKYITDSVDFTVYKRLATAAGNRAAQVP"
     gene            complement(2261..>3968)
                     /locus_tag="VFV87_03485"
     CDS             complement(2261..>3968)
                     /locus_tag="VFV87_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102844.1"
                     /translation="LQPAAESPAGRGEWLLPAMIVLLALLLGFVQMADTDIWWHLHTG
                     QLIWERGTVPRTDWFTYTNPDSPWIDLHWGFQLGAAGLWALGGVPALVIAKSIVGALT
                     FAICLTAQRPNWPSWHAAACWLPALLLFAGRYDVRPEMLSLLLFAVTLTILFHLRARP
                     RLVWLLPVVQLVWVNVHALFVLGLVLWMLFLIDAALRPFLPWKSPDSESGPPAWRSWL
                     AATGLQGAACLLNPYGWEGALFPLVLWSRLQGPQRTFYQQFAGEFLGFGDFLSQYGFG
                     AVFQNLSTAMLATMTIATAVSFVLLARYRQFSVFRALLFVAFGYLAWQANRNGVFYGL
                     AAGGVLRWNVGDLLAKRPATARQSFPIGKISVAAALGLLVILTALGALSPLRLLPGPR
                     FVPVFAELGLPPFAVVPRLMGVNETPLWYAHDAARFLRQPGMPRHVFASHLGHASLCI
                     YYLGPEKLVFADPRLEVNTHETLKRWLQINDALLSHDPAAERLLHECGGGELPALLIG
                     TADLWPVREQFFNHPRWQPVYVDPVAVIFLPTEQAEAMNLPRVEWTSIIVGDEPGREP
                     RD"
BASE COUNT          830 a         1235 c         1223 g          680 t
ORIGIN      
        1 tcgagcgggc gatcaagcgg gggacgggcg agctcgaagg gggcgaagtc gaggagatca
       61 tctacgaggg ctacgggccg ggcggcgtgg ccgtgctgtg cgagatcatg accgacaaac
      121 gcagccgcac cgccccggaa gtccgcaaga ttttcgacac gcacggcggc aagctcggcg
      181 ccaccaattg cgtcgcctgg atgttcgagc ggaagggcct gttgctggtg ccggcggcca
      241 agaccacgga ggagcagttg atggaggtcg cgctcgaagc gggggcggag gacctgaagc
      301 gcgaggggga catgtttcag atcgtctgct cgcccgaagc gttcaccgcc gtctccgacg
      361 cgctgaccgc cgcgggcatc gagccggcga gcaagcagct cacgcggatt ccgtccaata
      421 tcgtcgaggt ggccgacgtg gagacggccc gcaccgttct ggccctgatg gaagccctcg
      481 acgatcacga cgacgtacag aacgtctcgg cgaatttctc gattccagaa tcggcgctgg
      541 cggcactata agcgggcggc gcatctgcat tcgcgctcac ttctccaact cgaaatcgaa
      601 gacattcgat tcccccgcct tcacctctct ggtaagaccc gacgtctcgc cgctgcgata
      661 tttgacgttg aacggggcgg caacgacggg gccactcatc tgcttggcga cggctgtcga
      721 aggaggtggc tcggttttga tgctgaacga gaccttgtgc atgcccggca cggctccgtc
      781 cccggaagag atcgtggaca gctcatagtt gccgctggca tcggtctggc cgacggcagg
      841 ccggccattt tccggctgaa aattgacgat cacattcggc acgggctggc cgccgagagt
      901 gaccttgccc ctgacggttg ccgtatcgat tccagatccg caacccgtca ggagggctgc
      961 gaccgacagt gcaacaaccg cgtgcaatct ccactgcatg ccaacctgcc tttaccgtct
     1021 ctggcgatcc taaggcactt gggcggcccg gttgcccgca gccgttgcca gccgcttgta
     1081 gacggtaaag tctacagaat ccgtaatgta ttttacggcg ccatcgcaca tggcgaattg
     1141 cgccccgttg ggatggcggc tcataaagga atagttgttg agataatctg ctgcgttctc
     1201 ttccatcgtt tgcgtgccgt tcaaaacggc cgggggtttg taattgagag ggattccaca
     1261 agtggcggtc gaatggttcc agccccacca ggtcgtatga gtacaccagg cggggaccgc
     1321 ctcgccaacg gcaaacgtat tggacagccc atccgtgacg aacgccaact catgccacaa
     1381 agccgggttg ttgctgccgt tgcggcagat gatgccgttt cccgcgtcga gtccattgcc
     1441 gttgttcggg aagggcgccg ccttgacgtc ctccgtcacc tgcgcgccgg cactgctcca
     1501 ctggccggtg atcgcgcccg tcgatggatc ggcggtacac caattggcgc cggcacaggc
     1561 tttgtaattg gtcacgccgt agatggtagc cggattgacg ttcgcccggc cgctcatggc
     1621 gccgcggcca ttgttgccat ccgagataca cacgaacgac ttaatggtga cggtagcgac
     1681 cgcctggttt tctggactgc ccagcgtatg ggacgtgccg gacggagcgg gagtccagcg
     1741 gatctttttg tggagggtat cctgctcgat gaacggcaga atccccacca tccagccttt
     1801 gccccaatcc gtttcgctgg ccgtgccatc cccgctgtag caaatcggca gcgcggtgta
     1861 aatgtcgttg taattgtgca tcgcgatggc aagctgatgc atattgttgg agcagctcgc
     1921 tcgccggccg gcttcccgcg cggcctggac ggccggcaag aggagcgaaa ccaggatgcc
     1981 aataatcgca atcacgacca acagttccac caacgtgaag cctctagccc tgttcccgtt
     2041 cttcatagat caccccgtaa tcaagacgag catcaaggat cgatctttcg accacgatgc
     2101 tgacaccagc ggagagcacc gccggcgcgc gattctccct gacgctctat ctaccatcct
     2161 tgtaccccaa agcgaaatcg aattccactg gagacggcac tttctcgccc ccgcccgcaa
     2221 ttggggacgt tgatgtccag cccgacatgc catggccgga tcaatcgcga ggctccctgc
     2281 caggttcgtc cccaacgatt atggacgtcc actccactcg gggcagattc atcgcctcgg
     2341 cctgctcggt cggcaggaag attaccgcca cgggatccac gtaaacaggt tgccagcggg
     2401 ggtggttgaa gaactgttcg cgaaccggcc agagatcggc ggttccgatc aggagtgccg
     2461 gcaattcgcc gccgccgcac tcatgcagca gtcgctcggc agccgggtcg tgggatagaa
     2521 gcgcatcgtt aatctgcagc caacgcttca gcgtctcgtg ggtgttgact tccagtcgcg
     2581 ggtcggcaaa cacaagtttt tccggaccaa ggtagtagat gcacagactg gcgtgaccta
     2641 gatgcgaggc gaagacatgc cggggcatcc cgggctgacg gagaaaccgg gccgcgtcgt
     2701 gcgcatacca gagcggcgtt tcattgaccc ccatcagacg cggaacgacg gcgaacggcg
     2761 gcaggcccaa ttcggcgaac acgggaacga atcgggggcc gggcagaagt cgcagcggcg
     2821 acaacgctcc cagcgccgtc aggatgacga gcagccccag cgcagccgcc acggatattt
     2881 ttccaatcgg gaacgactgg cgcgccgtgg caggacgttt agccagcaaa tctccgacat
     2941 tccaccgcaa cactccgcca gcggccaggc cgtagaacac tccattgcga ttcgcttgcc
     3001 aggcaagata gccgaaggcg acgaacagga gcgcccgaaa aacgctgaac tggcgataac
     3061 gtgccaagag aacgaaactc actgccgtgg cgatcgtcat cgtcgccagc atggctgtgg
     3121 acaagttctg gaacaccgcc ccaaagccgt attgggaaag gaaatctccg aagcccagaa
     3181 actcacccgc gaactgctga tagaacgtcc gctgggggcc ctgcaaacgg ctccagagca
     3241 cgagcggaaa cagcgcgcct tcccagccgt agggattgag caaacacgcc gccccttgca
     3301 acccggtggc ggccagccaa ctgcgccagg ccggtggtcc gctttccgaa tcgggtgatt
     3361 tccagggtaa aaacgggcgg agcgctgcgt cgatcaaaaa aagcatccac aagaccagac
     3421 ccagcacgaa cagcgcgtga acattgaccc acaccagttg cacgaccggc agcagccaca
     3481 ccagccgcgg cctggcgcgg agatggaaca agattgtgag cgtcacggca aacagaagca
     3541 gcgagagcat ttccgggcgt acgtcatagc gtccggcaaa cagcagcagc gcgggcagcc
     3601 aacaagccgc agcatgccag gagggccaat ttggacgctg ggccgtcaga cagattgcga
     3661 acgtgagcgc gccgacgatg ctcttggcaa tcacgagtgc cgggacaccc cccagggccc
     3721 agagcccggc ggcgccaagt tgaaagcccc agtgcagatc gatccacggg ctgtcagggt
     3781 tggtgtacgt gaaccaatcg gtccgcggga ctgtgccgcg ctcccagatc agttgcccgg
     3841 tgtgaaggtg ccaccagata tcggtatccg ccatctggac gaatccgagc aagagcgcga
     3901 gcaggacgat catcgccggc agcagccact cgccccgacc agcgggcgac tcggcggccg
     3961 gctgtagc
//
LOCUS       DASUGB010000179         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_298016, whole genome shotgun sequence.
ACCESSION   DASUGB010000179 DASUGB010000000
VERSION     DASUGB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298016"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFV87_03490"
     CDS             <1..731
                     /locus_tag="VFV87_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX5102845.1"
                     /translation="TSSTFEAVIPALQHLGVTVLNVNTRGHDSVFGASLGNIRRRFGA
                     AYEIVDECRHDIAAWIEFLVSRGHVRIALIGHSLGAIKAVYAQAHERWREVAAVIAAS
                     PPRLSYAAFINAPESSTFFESFSTAEQMAKEGRAEDLFTSKFPFPLLITANAYIDKYG
                     PAERYNILGFAADLPCPALFIYGSKELEHGGIAFAGLPEALSSLPRAAPRQIVAIDGA
                     DHLYTGLGEQLAAEIASWLRSQGC"
     gene            858..1826
                     /locus_tag="VFV87_03495"
     CDS             858..1826
                     /locus_tag="VFV87_03495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102846.1"
                     /translation="MVSVASQPALARRKPDVRTVVRCYSLFDKFFPACGLLDYTEGIY
                     HNDPTTPYEEAQQNQIDYVLNEAGCMAGTRILEIGCGNGNLLESARRRRAKAVGITVS
                     PEQVALCRQRGLDARLKNYVDLDDRYFKQFDAVIANGPIEHFVQPAEAAAGRTDEIYR
                     EFFDICHRAIDPFSTVRRMITTTIHFVRPPDPHDLLKSPFRFRWGSDQFHYAMLARAF
                     GGWYPESGQFQRCNEGLFRLLHTVDGTYDYHLTSEEWLRRIRRVLPTKKGLQILASTI
                     PYALRHPVQYATMLTCMLGSQSWNWQFRGGKSAPTRLLRQTWGYRY"
     gene            complement(1873..>2757)
                     /locus_tag="VFV87_03500"
     CDS             complement(1873..>2757)
                     /locus_tag="VFV87_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX5102847.1"
                     /translation="PQDCLDQLALRMHEACRRGLPHIHRTLNPQELLLRAAAAVGGLA
                     PRVPWPPAIRSRIESRQTKRALARFFRARGCIYGPIRQPELLRCLPDLQRRKVLLLLR
                     DPRDILTSLYFSVSISHVEPGNPYFQAQFRERRQQSLSQSIDSFVRERTPKLLSDFRK
                     YCDFLHAHPNACLLRYEDMVRDFSGFLDNVSNYWGLSVDAPTRERLLALADFAVSTED
                     VHSHKRQVTPGDHRRKLSADSVRWLTDQFLDALETLGYGPEGEIRPLKCVTSLNQHLP
                     RPWTRMRDVPSNSRQRAA"
BASE COUNT          517 a          892 c          834 g          514 t
ORIGIN      
        1 acacctccag cacgttcgaa gcggtcattc ccgccctgca gcacctcggc gtcactgtgc
       61 tcaacgtcaa tacgcggggc cacgactcgg tcttcggcgc cagcctgggc aacatccgtc
      121 gccgctttgg cgcggcgtac gaaatcgtcg atgagtgccg ccacgatatc gccgcctgga
      181 tcgaatttct tgtcagccgc ggacacgtgc gcatcgccct catcggccac agcctcgggg
      241 cgatcaaagc cgtctatgcc caggcgcacg agcgctggcg tgaggtcgct gccgtcattg
      301 ccgccagtcc gccgcgcctg agctatgccg ctttcataaa cgccccggag agcagcactt
      361 ttttcgagtc gttcagcacc gccgagcaaa tggccaagga aggccgcgct gaggacctct
      421 tcacgagcaa gttcccgttc ccgctgctca tcacggccaa cgcctatatc gacaaatacg
      481 gccccgccga acgctacaac attctgggat tcgcagccga cttgccctgc ccggcgctct
      541 tcatatacgg cagcaaggaa ttggagcacg gcggcatcgc cttcgccggc ttgccggagg
      601 cattgtcttc gcttcccaga gccgcaccgc gacaaatcgt cgcgatcgac ggcgccgacc
      661 atctctatac tgggctcggc gagcaactcg ccgccgaaat cgcgtcttgg cttcgcagcc
      721 agggctgctg attgttgcgt ccgagcggca ggcatacaat ttgcgttatc gacgggttcg
      781 cgtctggccc aggcgtgtgc tcggttctgc aggcagccac cctggcattt gccaggcgcc
      841 tcgatctgga ggtgcccatg gtgtctgtcg cttcgcagcc cgctctcgct cggcggaagc
      901 ctgatgtccg cacggtcgtc cgctgctact cgctgttcga caagttcttc ccggcctgtg
      961 gactcttgga ctacacggag gggatctacc acaacgatcc caccacgccc tacgaagagg
     1021 cccagcaaaa ccagatcgac tatgtcctga acgaagccgg ctgcatggcc gggacgcgga
     1081 tcctcgagat cggctgcggc aacggcaacc tgctggagtc ggcgcgcagg cgccgggcga
     1141 aggcggtcgg catcaccgtc tcgcccgagc aggtcgccct ttgccgccag cgcgggctcg
     1201 atgcccgcct gaagaactac gtcgatcttg acgaccgcta tttcaagcag ttcgatgccg
     1261 tgatcgccaa cggccccatc gagcacttcg tccaacccgc cgaagcagcc gccgggcgga
     1321 cggacgagat ctatcgcgag tttttcgata tctgccaccg cgcgattgat cccttctcaa
     1381 cagtgcggcg gatgatcacc acgacgatcc acttcgtccg cccgcccgac ccccacgacc
     1441 ttttgaaaag ccctttccgc ttccgctggg gaagcgacca gttccactat gccatgctgg
     1501 cccgcgcgtt cggcgggtgg tatcccgagt cgggccagtt ccagcgctgc aatgaaggcc
     1561 tcttccgttt gctgcacacg gtcgatggca cgtacgacta tcacctcacc agcgaagaat
     1621 ggctccgccg catccgccgc gtcctgccga cgaagaaggg tctgcagatt ctggccagta
     1681 cgattcccta cgccctgcgg catccggtgc agtatgcgac gatgctcacc tgcatgctcg
     1741 gcagccagtc gtggaactgg caattccgcg gcggcaaatc ggcccccacg cgcctgctgc
     1801 ggcagacctg ggggtaccga tactgaattg tcgaagtgcg ccacaccaga aaggtgtgag
     1861 gagtcgatcg cgctaggctg cgcgctgcct ggagttagag ggtacgtctc gcatgcgtgt
     1921 ccagggacga ggtaaatgtt gattaagcga cgtgacgcat tttagcggcc ggatctctcc
     1981 ctccgggcca tatcccagcg tctccaacgc gtccaagaat tggtcggtca gccagcggac
     2041 ggagtcggcg gagagcttgc ggcggtgatc acccggcgtg acttggcgct tatgcgaatg
     2101 gacgtcctcg gtggagactg cgaaatcggc aagcgccagc agtcgctcgc gcgtgggtgc
     2161 gtcaacggac agcccccagt agttgctgac gttgtccaag aacccgctga aatcgcgaac
     2221 catgtcctca tagcggagca ggcaggcatt gggatgcgcg tgaaggaagt cgcagtattt
     2281 ccggaagtcg ctcaggagtt tgggggtccg ctcgcgtaca aagctgtcaa tgctttgcga
     2341 caaactctgt tgtctgcgct ctcggaattg cgcctgaaag tacgggtttc cgggttcgac
     2401 gtggctgatc gacaccgaaa agtagagcga tgtcagaatg tcgcgcggat cgcgaagaag
     2461 gagcagtacc ttgcggcgct ggagatccgg caggcatcgg agcagctcag gctgtcgaat
     2521 cggcccatag atacagccgc gggcacgaaa aaatcgggcc agagcccgct tcgtctggcg
     2581 agattcgatc ctggatcgga tcgccggagg ccaaggcacc cgcggcgcca atccgcccac
     2641 cgcagccgcc gcgcgcagca gcagttcctg cggattcaag gttcgatgaa tgtgcggcag
     2701 tccacggcga caggcttcgt gcatgcgaag tgcaagctgg tccagacaat cctgtgg
//
LOCUS       DASUGB010000180         6985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_159390, whole genome shotgun sequence.
ACCESSION   DASUGB010000180 DASUGB010000000
VERSION     DASUGB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159390"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFV87_03505"
     CDS             complement(<1..660)
                     /locus_tag="VFV87_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEX5102848.1"
                     /translation="MRRLWIAFTLVMGVSFLVLGWIGTRIYQEMPPIPAKVVSSDGTV
                     VVGEGEILAGQNVWQSLGGMEVGSVWGHGSYVAPDWTADWLHREATFILDRWANAEFG
                     SDFSKLDGEKQAQLQGRLAAVMRTNTYDPGTKSIALPVIRAEAFQANVKHYSDVFTNG
                     KAEYAIPAGAVTESARLKRLSAFFFWTSWAASTNRPNAEITYTHNWPYEPLVGNRPTG
                     ET"
     gene            complement(754..1071)
                     /locus_tag="VFV87_03510"
     CDS             complement(754..1071)
                     /locus_tag="VFV87_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102849.1"
                     /translation="MASPEEIAGPQPTGSDGMIEALRQFLRRRYGEPVLHVFDHRLAG
                     NEVRELVGPGTTGYAVKEAVKQIKAAAREFAANDPELASMIDRAFRSEAETVARRFSA
                     KTA"
     gene            1062..1331
                     /locus_tag="VFV87_03515"
     CDS             1062..1331
                     /locus_tag="VFV87_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102850.1"
                     /translation="MRPFLLLCQWRWLAVVVGGLLLLRQIDDRIAHAELESWQDRLAV
                     PAFEKRAWIDEQADHDFMRQWINVVISEASGPQSLRNVATPVHAE"
     gene            1721..2347
                     /locus_tag="VFV87_03520"
     CDS             1721..2347
                     /locus_tag="VFV87_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102851.1"
                     /translation="MILLLAADPSARFDKSGNRLDDARTVVNLTPEQEAAFRTIAEWE
                     EMVLTARSKTRRAEGARAVVRLSLHKHQTPLSDSELLAAQNVIFKLLDAQEVEVRRDA
                     ARTLGKLGFASSAPNLRQSAARDEDLFVRASCYQSLGLLKDGASIDLLAKAVVKEGPT
                     PAIEAAHALEAIGSAEAHAALADLSKKELNETVLEAVNKALDDLEFGN"
     gene            2384..3481
                     /locus_tag="VFV87_03525"
     CDS             2384..3481
                     /locus_tag="VFV87_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5102852.1"
                     /translation="MSDRRTRMVTCAPLLMVLTLLSAGALAAEMEMVKVAPDKKGFVL
                     SPSGQRFVPWGHNYASIDILQRLTEDPERVEREFAEMKAAGTSVARIHPELPRILAGA
                     EKVDPQAIEQLKKLLQIAEKSGIYLKITGLACYKIKDRAAWYDALVEQDRWRAQAFFW
                     ETVAKTCAESPAVFAYDLVNEPAAAGKLEDGWYTGRMGDVEFCQRLSLEPGDRNGDDI
                     FRGWTMRMVAAIRKHDQAHLITMGMLPFPGAYKAAAEQLDFVSPHLYPKSGKVDEEIK
                     LLQKFDWGKPIVIGETFPLSCGADDERDFLLKSREFAHGWIGHWPDESPAHLAELKKN
                     GKATIHNAIWLSWVELFKEVGPQMTANGKPE"
     gene            complement(3621..4628)
                     /locus_tag="VFV87_03530"
     CDS             complement(3621..4628)
                     /locus_tag="VFV87_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102853.1"
                     /translation="MAIWLVAASVSTSEAEPPRVHFDMPFAVACADVTSPQFAAANPG
                     QKLIEARLEISSLLVAGKERDLTQYFVRFESPQRTLTVVDYLPKTLHESRHAGPISVQ
                     DTDEKNASIGINLAGQYEMITGAGINAGLGHKNVSCVKQDLLPPLETVAASGTLLRGT
                     AVYFKLKSSPRNLLEGAREYALVLRVPCEWRADYVHVRCQAEGIERAFVSSLDQQVTC
                     GQRDFLVALYLEGDPGARDAAEQFSRREAQLRTSAQATEKGSETPSRHTAARNRPPRT
                     DDWLPRLLYGPAAQLAALPSELPQQVQQAVRDFAAARSDLEHLSGRSAQPPTGLAQSQ
                     P"
     gene            complement(4889..5638)
                     /gene="mtnC"
                     /locus_tag="VFV87_03535"
     CDS             complement(4889..5638)
                     /gene="mtnC"
                     /locus_tag="VFV87_03535"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEX5102854.1"
                     /translation="MLFHGRGILLDIEGTTSSVSFVYDVMFPYVRKHLTFEVLTNWME
                     PDYIDAFVAIAKDAGHGSLEAWLNSEGLTRDNPLRGANVVCREVTRLMDADAKATGLK
                     QLQGLIWKSGFESGELKAHVFDDVPPALARWNVAGKDTRIYSSGSVQAQKLFFGHTVA
                     GDLLSQFHGHYDTTTGPKKEAASYRRIAADFGLPAGEILFLSDVVAELDAARESGLQT
                     GLVIRPGNVTGQTSSGPAHAEIADFSQVEAS"
     gene            complement(5638..>6985)
                     /locus_tag="VFV87_03540"
     CDS             complement(5638..>6985)
                     /locus_tag="VFV87_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5102855.1"
                     /translation="PLPLELLAQWENNRRRALQGEVVWDELALQMPGGKRHYRYVSAP
                     IVDGSQIRGVVGVDIDVTEQRRAEQALRENEALFRLLAENSSDVISRHKPSGEWLYLS
                     PAAKTLMQYDPAELVGRDPFEFIHPDDHARAQAALMALIQTREPQSETFRIRRADGVY
                     LWSETDGRVVLDERGEIVELITTSRDITERIESSSKLRQREAELAQTGRLSAVGQMAS
                     EIAHEMNQPLYAIANFADASLTLLSQKPGQADASLRRWLEQIAQQARRAGEVLRRITQ
                     FARKGEFHPAALDLNDVVRAVLAMLDLEIGQQNVEIRRDLAGKLPAVSGDRLLIEQVL
                     VNLVRNALDALQAAQSGARIVTLRTRAEAEGVVLSVGDTGPGLSETQLARAFEPYFTT
                     KSDGIGLGLAICRSTIEAHRGRIWVRNNPEGGATFSIQLPRDGAAAASELHLPETA"
BASE COUNT         1306 a         2147 c         2199 g         1333 t
ORIGIN      
        1 tgtttctccc gtgggtcgat tgcccaccag cggttcatac ggccagttgt gcgtgtaggt
       61 gatctcggca tttgggcggt tggtggacgc cgcccaagaa gtccagaaga agaatgccga
      121 cagtcttttc aggcgggcgg attcggtcac ggccccggca ggaatggcat actcggcctt
      181 cccattggtg aacacgtcgg agtagtgctt gacgttcgcc tggaacgcct cggcccgaat
      241 gacgggaaga gcgatcgact tcgtgccggg gtcataggtg ttcgtccgca tcaccgccgc
      301 caagcggcct tgtagctggg cttgcttctc gccgtcgagc ttggagaaat cgctcccgaa
      361 ctcggcgttg gcccaacggt cgaggatgaa tgtcgcctcc cgatgaagcc aatcagcggt
      421 ccagtcggga gcgacgtagc tgccgtggcc ccacacagag ccaacctcca tgccacccag
      481 cgattgccat acattctggc ccgccagtat ctcgccctcg ccaaccacaa ccgtgccgtc
      541 gctcgacacc accttggccg gaatcggcgg catctcttgg taaatgcgtg tgccaatcca
      601 gcccagcacg agaaacgaga cccccatgac cagcgtaaag gcaatccaaa gtcgtcgcat
      661 cgaacctctc cttgaacagc cggggccgcc aagtggcgaa ttggtttccc gccgccatcg
      721 tcgcacttcc aggccaattc gccaagcggc gcatcacgcc gtcttcgcag agaatcttct
      781 cgccaccgtc tctgcctccg acctgaacgc ccggtcgatc atcgaggcca gttcagggtc
      841 gttggctgca aactcacggg cagcagcctt gatctgcttc acagcctcct tgaccgcata
      901 gccggtcgtc ccagggccga cgagttctcg cacttcattc ccagcaaggc gatgatcgaa
      961 gacgtgcaga accggctcgc cataccggcg tcgaaggaac tgacgtaacg cctcgatcat
     1021 cccgtcgctg cccgttggct gcggaccggc gatctcttcg ggtgaggcca ttcctgcttc
     1081 tttgccaatg gagatggctg gcagtcgtcg tcggcggtct gctgctgctg cgccagattg
     1141 atgaccgcat tgcgcacgct gagcttgaat cgtggcagga tcggctggcc gtcccagcct
     1201 ttgaaaagcg tgcctggatt gacgagcaag cggatcacga cttcatgcgt caatggatca
     1261 acgtcgtgat ctcggaagcc agcggacctc agagccttcg caacgtagcc acaccagtcc
     1321 atgccgagtg acctgctcca tctcggcttt tgtttctgga gaaccagcct gtgcgagcag
     1381 acttctcgca acttgttatg ctgcaaggac caagagcatt ttgatgtcgg ccgcccaggc
     1441 gtggacttct tcccagaagg ttgcggctgg tgcccagccg gttcgactgt ttgttggctt
     1501 tggacggttc tttggatgtt gtccgaaatc gcttggctcg ggcggggaag ccttgcattc
     1561 acccgctgtc tttccgcgct cctgcccgct ttttttccag cgtgctgggg aatggtcagt
     1621 cggacggtgt acaatgcgtc cgaggcaggc gagacgcctg cccctcggcg tgtacgttag
     1681 gcgggtcatt ttcggggagg gtgctgcgat gtctctattc ttgattctgc tgctcgctgc
     1741 cgatccgtcc gcgcgattcg acaagagtgg caatcgattg gacgatgcca ggacggttgt
     1801 gaacctcacg ccggagcaag aagccgcttt ccggacgatt gccgagtggg aagagatggt
     1861 gctgactgcg aggtcgaaga cgcgacgcgc agagggggcg cgggccgtgg tgcgcctcag
     1921 cctgcacaag caccagacgc cgctgagcga ttcggaattg ctggccgcgc agaatgtgat
     1981 cttcaagctg ctggatgctc aggaagtgga ggttcgccgc gacgccgcgc gaacgttggg
     2041 caagctgggc ttcgcctcct cggcgccgaa tttgcgccag agtgccgcca gggacgagga
     2101 cctctttgtg cgggcgagtt gttaccaatc gctcggtttg ctgaaggacg gagcctccat
     2161 tgatctattg gcaaaagcgg tcgtcaagga agggcccacg ccagccattg aggccgctca
     2221 cgcgctggag gccatcgggt cggccgaggc ccatgcggcc ctagcggatc tgtccaagaa
     2281 agaactcaat gaaacggttc tcgaagccgt caacaaggcg ctcgatgatc tggagttcgg
     2341 aaattgatcg ggcggtgatc acgcattgaa tggaggttcc gcaatgagcg atcgccggac
     2401 acgtatggtg acctgtgccc cgctgctcat ggttctgacg ctgctttcgg ccggcgcgct
     2461 ggcggcggag atggagatgg tgaaggtggc gccggacaag aagggcttcg ttctctcgcc
     2521 gtcgggccag cggtttgtcc cttgggggca taactacgcg tcgatcgata tcttgcagcg
     2581 gctgacggaa gatccggaac gggtcgagcg cgaatttgcc gaaatgaagg ccgccggcac
     2641 cagcgtagcg cggattcatc cggagttgcc gcggattctg gcaggcgcgg agaaggttga
     2701 tccgcaggcg atcgagcagt tgaaaaagtt gctgcagatt gccgagaaga gcggcattta
     2761 tttgaagatc accggtctgg cctgttacaa gatcaaggac cgcgcggcct ggtacgacgc
     2821 gctggtggag caagatcgct ggcgagccca ggcctttttc tgggagacag ttgccaaaac
     2881 ttgtgccgaa agcccggccg tcttcgctta tgacctggtc aacgagccag cagcagctgg
     2941 aaagttggag gacggatggt acacgggccg aatgggtgat gtcgaattct gtcagcggct
     3001 gagcctggag ccgggcgacc ggaatgggga cgacattttc cgtgggtgga cgatgcggat
     3061 ggtcgccgcg atccgcaagc acgaccaggc gcacctgatc acgatgggca tgctgccatt
     3121 cccgggcgct tataaggccg cggccgagca gctcgacttc gtctcgccgc acctatatcc
     3181 gaaatcgggc aaggtcgatg aggagatcaa gctgctgcaa aagttcgact ggggcaaacc
     3241 aattgtgatt ggcgaaacgt tccccttgag ctgcggcgcc gacgacgagc gcgacttcct
     3301 gctgaagagc cgcgagttcg cccacggctg gatcggccac tggccggacg aatcgccggc
     3361 ccacctggcc gagctgaaga agaacggcaa agccacgatc cacaatgcca tctggctttc
     3421 ctgggttgaa cttttcaagg aggtcggccc acaaatgacg gccaatggca agccggagta
     3481 aatcgccggt gttttggcgc cccaaaacta agtgccttgc ggcgactgcg tagatgaccc
     3541 cgaggatgag ttactttagg ggcaggcgga aagggtggaa agacaatcgg gaccggacga
     3601 actacgcagc aacgcgcgaa tcacggctgc gactgggcaa ggccggttgg cggttgagct
     3661 gagcggccgc tgagatgctc gaggtcgctg cgggcggccg cgaaatcgcg aaccgcttgc
     3721 tggacttgct gcggaagctc gctggggagc gcagcgagtt gggcggctgg gccgtaaagg
     3781 aggcgcggca gccagtcgtc ggtccggggc gggcgatttc gcgctgccgt gtggcgcgat
     3841 ggcgtttcag agcccttttc cgtggcctgg gcgctggtgc gaagctgcgc ctcgcggcgg
     3901 ctgaattgct cggcggcatc gcgggcccca ggatctccct ccaaatacag ggcgaccaga
     3961 aagtcacgct ggccgcaggt tacctgctgg tcgagcgagc tgacaaacgc ccgttcaatg
     4021 ccttccgcct ggcagcggac gtggacgtaa tcggcccgcc actcgcatgg cacgcgcagg
     4081 acaagggcat attcgcgggc cccttccagc aagtttcgcg gcgacgactt caacttgaaa
     4141 tagacggcgg tgccccgcag cagggtgccg cttgcggcga ccgtctccag cggcggcagg
     4201 aggtcctgtt tcacacagga gacgttctta tgccccaggc ccgcattaat gccggccccg
     4261 gtgatcattt cgtattgccc cgccaggttg atcccaatcg aggcgttctt ctcgtcggtg
     4321 tcttgcacgc tgattggacc cgcatgccgg ctttcgtgca gcgttttcgg caggtaatcg
     4381 acgactgtca gcgtccgctg cgggctttcg aagcggacga aatactgcgt cagatcgcgc
     4441 tccttccccg ccacgagaag cgacgagatc tccagccggg cctcaatcag cttctggccc
     4501 gggttggcgg cggcaaactg cggagaagtt acatcggcgc atgccaccgc gaacggcatg
     4561 tcgaaatgca cccgcggcgg ctcggcttcg gaagttgaaa cggacgcggc gactagccag
     4621 atcgccatca agctccctcg ctccatgcgg atcgactcct gtcgtaaggc aactgattgc
     4681 cggcaggcca aatcaaatga cccggcaatc gcacagcccg cgtgcggcgg gcgcctgccg
     4741 cagattccct catggagcgg ccagaaccgg cagccgagga attcatccgc cggctgctct
     4801 ccatcatcgt aggcgtggcc gttcgagagg caagccgcta cagattgact tttcgcgaag
     4861 gcagcgagcg catcgagaaa gccccatgct agctggcttc cacctgggaa aagtcggcaa
     4921 tctcagcgtg cgctggaccg gagctggtct gcccggtcac gttaccgggt cgaatcacaa
     4981 gccccgtttg caggccagat tcgcgagcgg cgtcgagctc agccaccacg tcgctcaaaa
     5041 acaggatctc gcccgcgggc aggccgaaat ctgccgcaat ccgtcggtaa cttgccgcct
     5101 ctttcttcgg tccggtcgtc gtgtcgtaat ggccgtggaa ctgcgacagc agatcgccgg
     5161 caacggtatg cccgaagaac agcttttgcg cctgcacgct cccggacgaa taaatccgcg
     5221 tgtccttgcc ggcgacattc cagcgcgcca gcgccggcgg cacatcgtca aaaacatgcg
     5281 ccttgagctc gccgctctcg aagccggatt tccagatcag cccctgaagc tgcttgagcc
     5341 ctgtcgcctt tgcgtccgca tccatcaggc gcgtgacttc ccggcacacg acgttggccc
     5401 cccgcagcgg gttgtcgcgc gtcaggcctt ccgaattcag ccaggcctcg agcgacccgt
     5461 gcccggcgtc cttggcaatt gcaacaaacg cgtcgatata gtccggctcc atccaattgg
     5521 tgagcacctc gaacgtgaga tgcttccgca catagggaaa catcacgtca taaacgaaac
     5581 tgacgctgga agtcgtcccc tcgatatcga gcaggattcc tcggccatga aagagcatca
     5641 cgcggtctcc ggtagatgga gttctgatgc agccgccgcg ccgtcacgtg gcaattggat
     5701 cgagaaggtg gcaccgccct ctggattgtt acggacccag atccggccgc gatgggcctc
     5761 aatggtcgag cggcagatgg ccaggcccag gccgatgccg tccgacttgg tggtgaaata
     5821 cggctcgaag gcccgggcga gctgcgtctc ggaaagtccc gggcctgtgt cgcccacgct
     5881 caggaccacg ccttccgctt ccgcccgcgt tcgcagggta acgattcgag ccccagactg
     5941 cgcggcctga agggcatcga gcgcgttgcg aaccagattg accagcacct gctcgatcag
     6001 caatcggtcg ccgctaactg ccggcagctt gccggccaga tcgcgacgga tttctacatt
     6061 ttgctggccg atttccaggt cgagcatcgc cagcacggcg cggacgacgt cgttcagatc
     6121 cagcgccgcc ggatggaatt cgcctttgcg cgcgaattgt gtgatccgcc gcaacacttc
     6181 gccagcccgc cgcgcttgct gggcaatctg ctccagccag cggcgcaggc tcgcgtcggc
     6241 ctgcccgggc ttttgcgaga gcagcgtcag cgaagcatcg gcaaaattgg cgatggcata
     6301 cagcggctga ttcatctcgt gggcaatttc cgacgccatc tgccccaccg cgctcaaccg
     6361 ccccgtctgt gccagctcgg cctcgcgctg gcggagcttg gagctggatt cgatccgctc
     6421 ggtgatatcg cgcgacgtgg tgatcagctc gacgatctcg ccccgctcat ccagaacgac
     6481 ccgaccatcc gtctcggacc agagatagac gccgtccgcc cgccgaatac ggaacgtctc
     6541 ggattgcggc tcgcgggtct gaatcagcgc catgagcgcc gcctgggccc gggcatggtc
     6601 gtcggggtgg atgaattcga acggatcgcg gcccaccagc tccgcggggt cgtactgcat
     6661 cagcgttttt gccgccggcg aaaggtacaa ccattcgcct gagggtttgt gccggctgat
     6721 gacgtcggat gaattctctg ccagcagccg gaagagcgct tcgttttcgc gaagcgcctg
     6781 ttcggcgcgg cgctgttcgg tcacgtcgat atcgacaccg acgacgccgc ggatttggga
     6841 gccgtccacg atcggcgcgc tgacatagcg atagtgccgc ttgccgccag gcatctggag
     6901 ggccaattca tcccacacca cctcaccttg cagagcgcgg cggcggttat tttcccactg
     6961 cgcgagaagt tcgagtggca agggc
//
LOCUS       DASUGB010000181         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_993518, whole genome shotgun sequence.
ACCESSION   DASUGB010000181 DASUGB010000000
VERSION     DASUGB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993518"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFV87_03545"
     CDS             complement(<1..243)
                     /locus_tag="VFV87_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5102856.1"
                     /translation="MTDGPRADDVVQATIAETGAIRRLADAREKIVSQLSQVIVGQND
                     VIEELLISLFARGHCLLEGVPGLAKTLMISTLARTLN"
     gene            complement(352..555)
                     /locus_tag="VFV87_03550"
     CDS             complement(352..555)
                     /locus_tag="VFV87_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102857.1"
                     /translation="MSQSLGEIKRSEEAKRERNWNAAARWKVLQETIRWAELQQTARR
                     NTPAACLARQRLLLAAGARGAGP"
     gene            complement(552..1034)
                     /locus_tag="VFV87_03555"
     CDS             complement(552..1034)
                     /locus_tag="VFV87_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102858.1"
                     /translation="MNVDHILSTFNQHQVEYLMIGGMHFLIRHAPLLTYDVDLWIEDS
                     EANRAKAERALAALDAEWGTTDADWGPVAAKSVGWLDRQPIFCLNSPHGAIDIFRAVA
                     GLTGWHSAAQYAISGTTAGGVPYRGLSDADMLQCQLALDPGQRKQDRIAILKQKLGIP
                     "
     gene            complement(1067..2014)
                     /locus_tag="VFV87_03560"
     CDS             complement(1067..2014)
                     /locus_tag="VFV87_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate dehydrogenase family protein"
                     /protein_id="HEX5102859.1"
                     /translation="MKCSIIGGGGLVGSSAGFALQCGGVVREIALLDVNQDLASGQAL
                     DLLHGGPSTADQVITSGSYEHVPSSDIVCITAGLRRKPDESRLDLINRNTDLFVSILD
                     EVKKAGLKKDAIVLVVSNPVDILTYVAADRLDLPPSQVLGLGTQLDTIRFRSLIAQEL
                     KVAPSQVNALILGEHGDSMVPIWSSAAIGGLPLDKFPGWSVRLADELFTRTKGSGAEV
                     IKKKTGAGFAVGIAIRDVIHSIALDQQRILPVSTVQNGCYDIRDVALSVPTVVGRGGC
                     VNRHAIDLWPKEVQLLRKSGLVLRQTLEQVLSRIGRTKK"
     gene            complement(2103..2963)
                     /locus_tag="VFV87_03565"
     CDS             complement(2103..2963)
                     /locus_tag="VFV87_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5102860.1"
                     /translation="MNVHRLKQDICEIGRRIYAKGFAAANDGNITVRIGENEILCTPT
                     MHCKGFLKPEDISTIDMTGKQIAGVKKRSSEALLHLEIYRQRPDVKSVVHCHPPHACA
                     FAIAREPIPQCILPEVEVFLGDVPITKYETPGGQEFANTIQPFVKKTNIILLANHGTV
                     SYGTDVERAYWWTEILDAYCRMLLLSKQLGRVNFFDEKKERELLELKDKWGWSDPRNT
                     KEYENCDICANDIFRDSWQNSGVERKAFPAPPPMGPNAAKAAASNGSGDTEAIVQMIT
                     QRVMEELAKR"
     gene            complement(2997..3278)
                     /locus_tag="VFV87_03570"
     CDS             complement(2997..3278)
                     /locus_tag="VFV87_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX5102861.1"
                     /translation="MRIAKVVGTVTLNRCHPSFNGASLRLIVPLSLAELKREQQPQAD
                     EMVAWDDHGAGIGSLLAISEGAEASQPFRPELKPVDTYASAILDSINIT"
     gene            complement(3283..3549)
                     /locus_tag="VFV87_03575"
     CDS             complement(3283..3549)
                     /locus_tag="VFV87_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102862.1"
                     /translation="MTFTEEQVAAIVVEVIRRLRLMENNQGVAVGERSSTTADLTLIE
                     KLVTTRTIENRLSGISRLLVTKRAVVTPAVNDELKKHQIELVRQ"
     gene            complement(3546..3863)
                     /locus_tag="VFV87_03580"
     CDS             complement(3546..3863)
                     /locus_tag="VFV87_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX5102863.1"
                     /translation="MDEQFALPGTAQRPFPTELMQLGLVVGTATSTVKHATLHGQKLL
                     VVQPLLADSSGADGDPQIAVDAVGAGRGQRVMITSDGRFVRELVKHENTPIRWSVIGI
                     ADA"
     gene            complement(3912..>4987)
                     /locus_tag="VFV87_03585"
     CDS             complement(3912..>4987)
                     /locus_tag="VFV87_03585"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX5102864.1"
                     /translation="LAVIEHAPFGVIGAITPVTHSLPTITGNAVSMIASGNTVVVNPH
                     PSGKKVAAEGVCRFNQAIYKDLGIDNLICVLAEPTLETANAIFAHRGINMICVTGGPA
                     VARAALKSGKRAVVAGPGNPPVVVDETADLDNAARCIIRGGSYDNNLLCIAEKEVFVV
                     NSVFDAMMQAMERAGAVRLNAREIETLTKNAIIQVGEGEHKHDAPSKDFLGQDAAVLA
                     KGAGKSGVSDKVELLFGETDLNNPFVPVEQMMPFVPFVRCKNVEEAIDRAKEAEHGFR
                     HTSIIHSNNVKNMTRMGRIMDTTLFVKNGPCMASLGLGGEGYLSFSIAGPTGEGVTTP
                     LTFTRERRCSLIDDLCILGKPKR"
BASE COUNT          835 a         1497 c         1636 g         1019 t
ORIGIN      
        1 gttcagcgtt ctggccaggg tgctgatcat cagcgtcttg gccagccccg gcacgccttc
       61 caggaggcaa tggccgcggg cgaagaggct gatgagcaat tcctcgatca cgtcgttctg
      121 gccgacgatc acctgcgaaa gctggctgac gatcttctcg cgggcgtccg ccaggcggcg
      181 aatcgcaccc gtctcggcaa tcgtggcctg aacgacatca tcggcacgcg ggccatcggt
      241 catgggaacc tcgtattcat cgtttaacgc gaagccgcag gcgtagcggt cgcatggcag
      301 acccaagtat agttcctgcc ctgagttagt gtaccggcgc tgcaggtttg ttcatggacc
      361 cgcgccacga gcgcctgcgg ccagcagcag cctctgccgg gctaaacacg cagcgggtgt
      421 attgcggcgg gcggtttgtt gcagttccgc ccagcgaatc gtctcctgga gaaccttcca
      481 gcgcgcggcc gcgttccaat tgcgctcgcg cttggcctct tcagaccgct tgatttcacc
      541 gagcgactgg ctcatggaat cccgagtttt tgcttcaaga tagcgatgcg atcctgcttg
      601 cgctggccgg gatccagcgc tagttgacat tgtaacatgt cggcatcgga gaggccgcga
      661 tagggcacgc cacccgcagt cgtgccactg atggcatact gagccgccga gtgccagcca
      721 gtcagacccg caacggcgcg aaagatgtcg atggccccat gcgggctgtt gaggcagaaa
      781 atgggctgtc gatcgagcca gcccacagac ttggccgcca cgggccccca gtcggcatcc
      841 gtcgtgcccc actccgcatc gagggctgcc agcgcccgtt ccgccttggc gcgatttgct
      901 tccgagtcct caatccacaa atcaacgtcg taggtcaaca gcggcgcgtg gcgaatcaaa
      961 aagtgcattc ctccaatcat caagtattcg acttgatgct gattgaacgt gctcagaatg
     1021 tgatcgacgt tcatcgcggc agtttaccaa agacctttcc gagggatcac ttcttcgtcc
     1081 gcccgatccg gctgaggact tgctcgagcg tctggcgtag gacgaggccg ctcttgcgga
     1141 ggagttggac ttccttgggc cagaggtcga tggcgtggcg gttgacgcac ccgccacggc
     1201 cgacgacggt gggtacgctg agggccacgt cgcgaatgtc gtagcagccg ttctggacgg
     1261 tcgagaccgg caggatgcgc tgctggtcca aggcgatcga gtggatcacg tcgcggatgg
     1321 cgatgccgac cgcgaacccg gcgcccgtct tcttcttgat gacctccgcg ccggagcctt
     1381 tggtccgcgt gaacagctcg tcggcaagac gcacgctcca gccggggaat ttatcgagcg
     1441 gcaggccgcc gatggcggcg ctggaccaga tcgggaccat gctgtcgccg tgctcgccga
     1501 ggatcagggc gttcacttgc gacggggcga ccttgagttc ctgggcgatc aggctgcgga
     1561 agcggatcgt gtcgagctgc gtgccgaggc ccagcacctg gctcggcggc aggtcgaggc
     1621 ggtcggcggc gacgtaggtg aggatatcga ccgggttgct cacaaccagg acgatcgcgt
     1681 ccttcttcaa gccggccttc tttacctcgt caagaatcga gacgaacagg tccgtattgc
     1741 ggttgatcag gtccaggcgt gattcgtccg gcttgcggcg gaggccggcg gtgatgcaga
     1801 cgatgtcgct cgagggcaca tgctcgtagc tgcccgacgt gatgacctga tccgccgtgc
     1861 tcggcccgcc gtgcagcagg tcgagcgcct ggccgctggc gaggtcctga ttgacgtcga
     1921 gaagggcgat ctcgcggacc acgccgccgc attgcagggc aaagcccgcg ctggagccga
     1981 caagtccgcc gccgccgatg atggagcatt tcatagagga cagaggacag aggacagggg
     2041 acaggggaca ggactcgttc ccacgctctg cgtgggaacg catgctcgga cgctccgcgt
     2101 ccctaccgct tcgccaactc ctccatgacc cgctgggtga tcatctgcac gattgcttcg
     2161 gtgtcgccgc tgccgttcga cgcggcagcc ttcgccgcgt tgggacccat cggcggcggg
     2221 gcggggaagg ctttgcgttc gacgccggaa ttctgccaac tgtcgcggaa gatgtcgttg
     2281 gcgcagatgt cgcagttctc gtattccttg gtgttccgcg ggtcagacca gccccatttg
     2341 tccttcagct ccagcagctc gcgctctttc ttctcgtcga agaagttcac gcggccgagc
     2401 tgcttgctta agagcaacat ccggcagtag gcgtcgagga tttcggtcca ccagtaggcc
     2461 cgctcgacat cggtgccgta gctgaccgtg ccgtggttgg cgagcaggat gatgttggtc
     2521 ttcttgacga atggctggat cgtgttggcg aactcctggc cgccgggcgt ttcgtatttg
     2581 gtgatcggca cgtcgccgag gaagacctcg acctcgggca ggatgcactg cgggatcggc
     2641 tcgcgggcga tggcgaaggc acaggcgtgc ggcgggtggc aatgcacgac gctcttcacg
     2701 tccggccgct ggcgatagat ctccaggtgc agcagggcct cgctggaccg cttcttcacg
     2761 ccggcgattt gcttgccggt catgtcgatc gtgctgatgt cctccggctt gagaaagcct
     2821 ttacagtgca ttgtcggcgt gcagaggatt tcgttctcgc cgatgcggac ggtgatgttg
     2881 ccgtcgttgg cggctgcgaa gcccttggca taaatgcggc ggccgatttc gcagatgtct
     2941 tgcttgagac ggtggacgtt catgggaatt gcagattacg gatttgggat tgcggattag
     3001 gtgatattga tggagtcgag aatcgccgac gcgtaggtat cgacgggttt cagttcggga
     3061 cgaaacggct ggctggcttc ggcgccttcg ctgatggcga gcaggctgcc gatccccgcg
     3121 ccgtggtcgt cccaggcgac catttcgtcg gcctgcggct gctgctcgcg tttcagctcg
     3181 gcgagcgaca gcggcacgat gagccgcaag ctggccccgt tgaaactggg gtggcagcga
     3241 ttgagcgtca cggtgccgac gactttggca attcgcatgg gattattggc ggaccaactc
     3301 gatctggtgt ttcttcagtt cgtcgttgac ggcgggagtc acgacggctc gtttggtcac
     3361 gagcaggcgg ctgatgccgg aaagacggtt ttcgatggtt cgggtcgtaa ctagcttttc
     3421 gattagcgtc aggtcagccg tggttgagct tcgctcaccc acagccaccc cttggttgtt
     3481 ttccatcaat cgcagtcggc ggatgacttc gacgacgatc gcggcgactt gctcttcggt
     3541 gaaagtcatg cgtcggcaat cccaatcacg gaccagcgga tcggcgtgtt ttcgtgcttc
     3601 accagttcgc ggacaaaccg gccgtcgctc gtgatcatca cgcgttggcc ccggcccgcg
     3661 ccaaccgcat cgaccgcgat ctgcggatcg ccgtcagcac cgctgctatc ggcaagcagt
     3721 ggctgcacca cgagcagctt ctgcccgtgc agcgtggcgt gcttcacggt cgatgttgcg
     3781 gtgccgacga ctaatccaag ttgcataagt tctgtaggga acggcctctg tgccgttccg
     3841 ggaagtgcga attgttcgtc catcccgccg cacggaacgc cacggagggc gttccctaca
     3901 gcgcgtcagc cctaccgttt cggcttgccg aggatgcaca aatcgtcgat cagcgagcac
     3961 cgccgttcgc gcgtgaacgt gagcggcgtc gtcacgcctt cgccggtggg gccggcgatc
     4021 gagaacgaga ggtagccctc gccgcctaag ccgagcgacg ccatgcaggg gccgtttttc
     4081 acgaacaggg tcgtgtccat gatgcggccc atgcgggtca tgtttttcac gttgttcgag
     4141 tggatgatgc tcgtgtgccg aaagccgtgc tcggcttctt tggcccggtc gatggcctct
     4201 tcgacgttct tgcagcgcac gaacgggacg aacggcatca tctgctcgac gggaacgaac
     4261 ggattgttca agtcggtctc gccgaagagc aactcgacct tatcggagac gccgctcttg
     4321 ccggcgcctt tggcgaggac ggcggcgtcc tggccgagga agtccttgct cggagcatcg
     4381 tgcttgtgct cgccttcacc gacttgaatg atcgcattct tggtcagcgt ctcgatctcg
     4441 cgggcattca gccgcacggc gccggcccgt tccatcgcct gcatcatcgc atcgaagacg
     4501 ctattaacga cgaaaacttc cttttcggcg atgcagagca ggttgttgtc atacgaaccg
     4561 ccgcgaatga tgcaccgcgc ggcattgtcc agatcagcgg tttcatcgac gacgaccggc
     4621 ggattgcccg gcccggcgac gaccgcacgc ttgcccgact tgagcgccgc ccgcgcgacg
     4681 gcggggccgc ccgtgacgca gatcatgttg atcccgcggt gggcaaagat agcgttggcg
     4741 gtttcgagcg tcggctccgc caggacgcag atcaggttgt cgatgcccag gtctttgtag
     4801 atcgcctggt tgaagcggca gacgccctcg gctgcgactt tcttgccgct gggatgcggg
     4861 ttgacgacga ccgtgttgcc ggaggcgatc atgctcacgg cgttgccggt gatggtcggc
     4921 agcgagtggg tgaccggggt gatggcgccg atgacgccga agggggcgtg ctcgatgacc
     4981 gccaggc
//
LOCUS       DASUGB010000182         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_298121, whole genome shotgun sequence.
ACCESSION   DASUGB010000182 DASUGB010000000
VERSION     DASUGB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298121"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /gene="sucC"
                     /locus_tag="VFV87_03590"
     CDS             <1..1155
                     /gene="sucC"
                     /locus_tag="VFV87_03590"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5102865.1"
                     /translation="LFRKAGVAVLRGKVAAKPEEAAAAFSELGSPIAVVKAQIHAGGR
                     GKGTIQDNPSQRGVQLVKSADEAKKVAGNLLGKTLVTIQTGAEGKTVNRVFIEEGCRI
                     ARELYLGIVLDRAAAKPVLMVSTEGGVEIEKVAAETPHLIHKEHFDPHFGLQPYQVRK
                     LCYKLGIKGASVASAEKFMRALCRMYVQLDCSLVEINPLVVTESGELIALDGKISFDD
                     NAMFRHKDLAELRDLSEEDPAEIRASNTGLSYVKLDGNIGCLVNGAGLAMSTMDIIKL
                     HGGEPANFLDVGGGANEQQVTEAFRIILSDPNVKAILVNIFGGIARCTTIAAAIVAAS
                     KAVGFTVPLVVRLEGTEVEEGKRILRESGVKLITADDLTDAAKKVVASVK"
     gene            1307..2149
                     /gene="sucD"
                     /locus_tag="VFV87_03595"
     CDS             1307..2149
                     /gene="sucD"
                     /locus_tag="VFV87_03595"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5102866.1"
                     /translation="MSILVNKNTRVICQGITGKAGAFHTKGCKDYGTNMVGGVTPGKG
                     GEKVEGVPVFDTVAEAVEKTGANATMIFVPAAFTADAILEALDAGIKVICAITEGVPV
                     LDMVRVYEIVRASDAVLIGPNCPGVITPGECKIGIMSRSGTLTYEAVWQTSNLGLGQS
                     TCVGLGGDPIVGTSFVDLFKLYQADPGTEAILMMGEIGGTAEEEAAAFVKQHVTKPVA
                     AFIAGRTAPPGRRMGHAGAIISGGKGTAAEKVAALQDAGIEVADSPADMGAAVKRALE
                     KRGR"
     gene            2169..3011
                     /locus_tag="VFV87_03600"
     CDS             2169..3011
                     /locus_tag="VFV87_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEX5102867.1"
                     /translation="MSQAPAWLTIESDCLGSPDFPPAETKAATIYQILDWLQTKGLLT
                     PSGVERAKDRSAESWPTPGIYRDDVKPEAHPFLDREFTLILPIDIQMVTMMPQFIESI
                     LPYKLDDAWGRFTGDKYRDLKARLDDAQSNGSDSDLCNAVAVIIDQCQWFFPETKKCL
                     ADQWTVKSVWAAAGIIGNGGFEYLFSHEWDDDLDFLRTMKAFDAIECPQALDAMRAAF
                     RLFQHGKPPRDSDTRMTEFRQVPESTRDAVSSEFFRVLNSGAIERALAAFIRANPKSF
                     TVSN"
     gene            3290..3949
                     /locus_tag="VFV87_03605"
     CDS             3290..3949
                     /locus_tag="VFV87_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102868.1"
                     /translation="MSGYPQQHLPGQQPYPAQAPQGNPFAEQVNPYAAPPIAGYQPQM
                     AGVGNNPFAGLWRQGNVLVMHKLAPLPDICLLSNQPATRRLKRTMYWHNPLFYIIVLI
                     SPLIYIIVALIVQKNATIQMPLTEEWYGRRQRRMLFAWGAALAGAALIGGAIALSDAL
                     GDSAALIAVGGILLALGGLIYGLIACRLVTPERITDQYVFIKGVNHDFLNRLEPWMWN
                     V"
     gene            4032..4520
                     /locus_tag="VFV87_03610"
     CDS             4032..4520
                     /locus_tag="VFV87_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102869.1"
                     /translation="MRQDANTPPMGEPLAYFLTWTTYGTWLPGDERGWVKRPGSLEEA
                     NPRLERWSRSKLAERALILAPGQRAIVERTIVDHCRIRGWHLWIVRCLSNHVHVVITA
                     RDVDPDGVVRQLKAWCTRRLKEADAAEEARVHWWTERASTRYINDRNGLEGAILYVRD
                     AQ"
     gene            4578..>4786
                     /locus_tag="VFV87_03615"
     CDS             4578..>4786
                     /locus_tag="VFV87_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102870.1"
                     /translation="MTEINPYASPTVAAGYDASAGPGIGVWRDGDMFVIHRRAALPAV
                     CIETGQPAEAWRTFNVAGNNPFAWSA"
BASE COUNT          943 a         1455 c         1558 g          830 t
ORIGIN      
        1 ctctttcgca aggcgggcgt tgcggtgctc cgcggcaagg tggcggcgaa gcccgaagag
       61 gcggcggcag cgttctccga gctgggcagc ccgatcgccg tagtcaaggc gcaaatccac
      121 gccggcggcc gcggcaaggg aacgatccag gacaacccca gccagcgtgg cgtgcagctc
      181 gtaaaaagcg cggacgaggc gaagaaggtc gccggcaatc tgctcggcaa gacgctcgtc
      241 acgatccaga ccggcgcgga aggcaaaacg gtcaatcgcg tcttcatcga ggaaggctgc
      301 cggatcgccc gcgaactgta cctgggcatc gtcctcgacc gggccgccgc caaacccgtg
      361 ctgatggtca gcacggaggg cggcgtcgag atcgagaagg tcgctgccga aactccgcac
      421 ctgatccaca aggaacattt cgatccgcac ttcggcctcc agccgtatca ggtccgcaag
      481 ctgtgctaca agctcggcat caagggtgcg agcgtcgcca gcgcggagaa gttcatgcgg
      541 gccctgtgcc gcatgtacgt ccagctcgat tgcagcctgg tcgagatcaa cccgctggtc
      601 gtgaccgaaa gcggcgagct gatcgcgctc gacggcaaga tcagcttcga cgacaacgcc
      661 atgttccgcc acaaggacct ggccgaactg cgcgacctct cggaagaaga cccggcggaa
      721 atccgggcca gcaacacggg cctgtcgtat gtcaagctcg acggcaacat cggctgcctc
      781 gtgaatgggg cggggctggc gatgtccacg atggacatca tcaagctcca cggcggcgag
      841 ccggcgaatt tcctcgacgt cggcggcggc gccaacgagc agcaagtcac cgaggccttt
      901 cgcatcatcc tctccgaccc gaacgtcaag gcgatcctgg tcaacatctt cggcggcatc
      961 gcccggtgta cgacgatcgc cgcggccatc gtcgccgcca gcaaggccgt cggcttcacc
     1021 gtcccgctgg tcgtccgcct ggaagggacc gaagtcgaag aaggcaaaag gatcctccgc
     1081 gaaagcggcg tgaagttgat tactgccgac gacctgaccg acgcggcgaa gaaggtcgtc
     1141 gcctccgtga agtaaaccaa cccctcgttc ccgcgctccg cgtaggaacg cccgcccgga
     1201 cgctccgcct cccgccggcg acgcagagcg cgacgtgggt tcccacgcag agcgtgggaa
     1261 ccagtagttg aacgacaagt gacaaatgac aagtgacaag tgaccaatga gcattctcgt
     1321 taacaagaac acgcgtgtga tctgccaggg catcaccggc aaggcgggcg cgttccatac
     1381 caagggctgc aaggactacg gcaccaacat ggtcggcggc gtcacgccgg gcaaaggcgg
     1441 cgagaaagtt gaaggcgtgc ccgtcttcga cacggtcgcc gaagcggtcg agaagaccgg
     1501 ggcgaacgcc acgatgatct tcgtgccggc ggcgttcacg gcggacgcga tcctcgaggc
     1561 gctcgacgcg gggatcaaag tgatttgtgc gatcaccgaa ggggtgccgg tgctcgacat
     1621 ggtccgcgtt tatgaaatcg tccgcgccag cgacgccgtg ctgattggcc ccaactgccc
     1681 gggcgtgatc actccgggcg agtgcaaaat cggcatcatg agccgcagcg gcacgctgac
     1741 gtatgaggcg gtctggcaga cgagcaacct gggcctcggc caatcgacct gcgtgggcct
     1801 cggcggcgac ccgatcgtcg gcaccagctt cgtcgatctg ttcaagctct atcaggccga
     1861 ccccggcacc gaagcgatcc tgatgatggg cgagatcggc ggcaccgcgg aggaggaggc
     1921 ggctgcgttc gtcaagcagc acgtcaccaa gccggtcgcc gcgttcatcg ccggccgcac
     1981 ggctccgccc ggccgccgga tgggccacgc cggcgcgatc atcagcggcg gcaaaggcac
     2041 cgccgccgag aaagtcgccg ccctccagga cgccggcatc gaagtcgccg acagccccgc
     2101 cgacatgggc gccgcggtca agcgggcgct ggaaaagcgg gggcggtgaa gcacgcggga
     2161 ttccgtccat gtctcaagct cctgcctggc taacgattga gagcgattgc ctgggtagcc
     2221 ccgactttcc tccggccgaa accaaggctg cgacgatcta ccagattctg gattggctgc
     2281 agaccaaagg cctgctcacc ccgtctggcg tcgagcgggc caaggatcgg tccgcggaga
     2341 gttggccaac gcccggcatt tatcgcgacg atgtgaaacc agaggcgcac cccttcttgg
     2401 atcgcgaatt cacgttgata ctgccgatcg acatccagat ggtgactatg atgccgcaat
     2461 tcattgaatc tattttgccc tacaagctcg atgacgcatg gggaaggttc accggcgata
     2521 agtatcgtga tttgaaggca cggttggatg atgctcagtc gaacggaagt gacagtgatc
     2581 tctgcaatgc agtggcggta atcatcgacc aatgccagtg gttctttcct gaaaccaaaa
     2641 agtgcctggc ggatcagtgg actgtcaaat ccgtgtgggc cgcagctgga atcatcggca
     2701 atggcggatt tgagtacttg ttctcgcatg agtgggatga cgatctggat ttcctgcgaa
     2761 caatgaaggc atttgacgca atcgaatgtc cgcaggctct ggatgcgatg cgtgcggcgt
     2821 ttcgattatt ccagcacggc aaacctccgc gtgattcgga cacgagaatg acggaattca
     2881 gacaagttcc tgaatcaaca cgcgatgcag tcagtagcga gttcttcagg gtcttgaact
     2941 ccggtgccat tgagcgcgct ttggctgcgt tcattcgtgc gaatcccaag tcattcaccg
     3001 ttagcaacta gccgcttcgt cgcggagcga cgcaccaacc gtggccgggc ctttccaggc
     3061 ccggtttcgt tgcttggccg atggcaagct gctgacgttt gggatgcgcg gcaagaatcg
     3121 cgaggcagcc ggcggcgagc ggatatcgag cgttcacggg ctgagggtcc ccgggtcgag
     3181 cgaacgcgcg atgtgggttc gcaaggtttg tggttcggcc cagccgccag tatattgacg
     3241 cggcggggcc ttcgcccaac cgacttcacg ccacttcgcg atcactgcca tgtccggcta
     3301 tccccagcag caccttccgg gtcagcagcc ctatccggcg caggctcccc aaggcaatcc
     3361 gtttgccgag caagtcaatc cttacgccgc gccgccgatt gccggctatc agccgcagat
     3421 ggcgggtgta ggtaacaatc cgttcgcggg cctgtggcga caggggaacg tgctggtgat
     3481 gcacaagttg gccccgctgc cggatatatg cctgctgagc aaccagccgg cgacgcggcg
     3541 gctgaagcgg acgatgtatt ggcacaatcc attgttctat atcattgtgc tgatcagccc
     3601 tctgatctac atcatcgtgg cgctgatcgt gcagaagaac gcgacaatcc aaatgccgct
     3661 cacggaagag tggtacggcc gccgccagag gcggatgctg tttgcgtggg gcgcggcact
     3721 ggcgggcgcg gcgctgatcg gcggggccat tgccctgagc gacgcgctgg gcgacagcgc
     3781 cgctttgatc gccgtgggcg gcattttgct ggctctgggg gggctcatct acggcctgat
     3841 cgcctgccgc ctcgtcacgc ccgagcggat caccgaccag tacgtcttca tcaagggcgt
     3901 gaatcacgat ttcctgaacc ggctggagcc gtggatgtgg aatgtgtgaa cgtggcccct
     3961 cttcgggctt gtgccgcgtg ccacacaagc ccgaagcgca agcgaggggc tgagccgagc
     4021 gaggatcaac catgcgtcag gacgccaaca cgccgccgat gggtgagccg ctggcgtatt
     4081 ttctgacctg gacgacttac ggcacgtggc tgccggggga cgaacgaggt tgggtgaagc
     4141 ggcccgggag tctggaggaa gccaatccaa gattggaacg ttggtcgagg agcaaacttg
     4201 cggaacgggc acttatactc gcaccgggac agcgcgcgat cgtcgaacgg accatagtag
     4261 accattgccg gattcgcggc tggcacctat ggattgttcg ctgtttgagt aatcacgttc
     4321 atgtcgtcat caccgcgagg gacgtcgatc ccgatggtgt cgtgaggcaa ctcaaagcct
     4381 ggtgcaccag gagactcaag gaggccgacg cagccgagga ggctcgcgta cattggtgga
     4441 ccgagcgggc cagcacgcgg tacatcaatg accggaatgg cttggaaggc gcgatcctgt
     4501 acgtgcgcga tgcgcagtga ccaggcggtt tgtgcccctc gcttgcgctt cgggcttgtg
     4561 tgcgcggagg aactgtggtg accgagatca acccttacgc ctcgccaacc gtagccgccg
     4621 gctacgacgc ctcggccggc cccggcatcg gcgtctggcg cgatggggac atgtttgtca
     4681 tccaccggcg ggcggcgctg cctgccgttt gcattgagac gggacagccg gccgaggcct
     4741 ggcgaacgtt taacgtggcg ggaaacaatc cgtttgcctg gtccgc
//
LOCUS       DASUGB010000183         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1688319, whole genome shotgun sequence.
ACCESSION   DASUGB010000183 DASUGB010000000
VERSION     DASUGB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688319"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFV87_03620"
     CDS             complement(<1..198)
                     /locus_tag="VFV87_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102871.1"
                     /translation="MLDLATTIVLIAHLLCVNVAAGAPILCVWLEWRGDRLARKAAAY
                     LGGMGLVTLIAGGLLGLAVGWL"
     gene            408..1460
                     /locus_tag="VFV87_03625"
     CDS             408..1460
                     /locus_tag="VFV87_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5102872.1"
                     /translation="MLSAVIPLRDEEESLAALHGELAAAAREQGYDLEIVFIDDGSTD
                     GSWEVIERLAASDPRVRGIRFRRNFGKAAALSAGFDAARGDLVVTLDADLQDDPREIP
                     RFLAKIDEGLDCVSGWKQVRHDPWHKVGPSRVFNWLVGALTGVKLHDHNCGFKCYRRE
                     IFDEVRLYGELHRFVPVLAAARGWKVGEIVVNHRARQHGRSKYGLSRIVKGFLDLFTV
                     YFLTGFEQRPQHLLGTCGIAAFALGLAGLMYLTFTWLIANATDWNERVSEAWQFKPLH
                     QRPALLYSLGMLLLGAQFISMGFLAELFTAYYGRSARPYSIKSRLNLPESVVGTRRVP
                     ASDNGTQDAPATSLRP"
     gene            1457..>2442
                     /locus_tag="VFV87_03630"
     CDS             1457..>2442
                     /locus_tag="VFV87_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102873.1"
                     /translation="MTAACIAAASGRAPCIAATSGRAPSVASDGIAAGGSAGASPSRF
                     RWSLYTLLIVVAVGLVTARIVRNRSPDAFSPTPFQSANDKSRWCTIRALGDDGVYSID
                     HIVFYEGGQQVRGWHTIDLVRHRGPDGREHYYSSKPTLLATLLAGEYVVVKRVTGLSL
                     SAPRQVWAVSRLMLVLTNVVPLAIALYLLARLIDELGATDWGRLFTLACAALATFITT
                     FSSTLNNHSLAACTLVFALAAAVPIWQGRGRWWHFALAGLFFAFTAANELPALSMLVL
                     AAAAFGWRFPAKTAIAFAPPALLVAAAALGTNYDAHGDWRTPYAHRQDGATIG"
BASE COUNT          386 a          875 c          748 g          433 t
ORIGIN      
        1 cagccagccg accgccagac caagcaagcc gcccgcgatt agcgtgacca gccccatgcc
       61 gcccaggtac gccgccgcct tgcgcgccag tcgatcgccg cgccattcga gccagacaca
      121 caaaatcggc gcgcccgccg caacgttcac gcacagcaag tgtgcaatca acacgatggt
      181 ggtcgccagg tccagcacgt ttcaccacaa gcacgaggcg cgaattctgg gacgacatta
      241 taggagcaaa actccggcag acgccccgca aactttcgac tatcctaaaa ccatcgagtc
      301 ctctggccgc tgtcctctta gtccactccc tcgctggcgc ttcgggctag ttttagccct
      361 gtcgtctgac ccccgtcctc tgaaccccga accccgaacc ctgccccgtg ctctccgccg
      421 tcatcccgct ccgcgacgag gaagaaagcc tggcggcctt gcacggcgaa ttggccgccg
      481 ccgcccgcga gcagggctac gacttggaga tcgtgttcat cgacgacggc tcgaccgacg
      541 gctcgtggga ggtgatcgag cggctcgcgg caagcgatcc gcgcgttcgc ggcattcgct
      601 tccgccgcaa tttcggcaaa gcggccgctc tgagcgcagg gttcgatgcg gcccgcggcg
      661 acctggtcgt caccctcgat gccgaccttc aggacgaccc ccgcgagatt ccccgcttcc
      721 tggccaagat cgacgaaggg ctcgactgcg tcagcggctg gaagcaggtc cgccatgacc
      781 cctggcacaa agtcggcccc tcgcgggtct tcaactggct ggtcggcgct ctgaccggcg
      841 tcaagctgca cgaccacaac tgcggcttca aatgctatcg ccgcgaaatt ttcgacgaag
      901 tgcggctgta cggcgagctg caccgctttg tccctgtgct ggccgccgcc cgcggctgga
      961 aagtgggaga aattgtcgtc aaccaccggg cccgccagca cggccgctcg aaatatggcc
     1021 tgtcgcgcat cgtcaaaggc ttcctcgacc tgttcaccgt gtatttcctc accggcttcg
     1081 agcagcggcc gcagcacctc ttgggcacgt gcggcatcgc ggcgttcgcc ctgggcctgg
     1141 cgggactgat gtacctgacg ttcacctggc tgatcgccaa cgcgaccgac tggaacgagc
     1201 gggtctccga ggcctggcag ttcaaaccgc tgcaccagcg gccggccctg ctctattcgt
     1261 tgggtatgct gctgctgggc gcgcagttca tctcgatggg cttcctggcc gagctgttca
     1321 ccgcgtatta cggccgcagc gcccgtccgt attcgatcaa gtcccgcctc aacctgccgg
     1381 aaagcgtggt aggcacgcgc cgcgtgccgg ccagcgataa cggcacgcag gacgcgccgg
     1441 cgacgagctt gcgaccatga ccgccgcctg catagccgcg gccagtggtc gcgctccgtg
     1501 catagccgcg accagcggtc gcgctccgag cgtcgcgtcc gatggaattg cggcgggcgg
     1561 ctcggcggga gcctcgccct cccggtttcg ctggtcgctc tacacgctgc tgatcgtcgt
     1621 cgccgtcggc ctggtcaccg cccgcatcgt gcgcaatcgc tcgcccgacg ccttcagccc
     1681 cacgccgttc caatcggcca acgacaaaag tcgctggtgt acgatccggg ccctcggcga
     1741 cgacggcgtc tattccatcg accacatcgt gttttacgag ggtggccagc aagtccgcgg
     1801 ctggcacacg atcgacctgg tgcggcaccg cggcccggac ggccgggagc actactactc
     1861 gagcaagccg acgctgctgg cgacgctctt ggccggcgaa tacgtcgtcg taaagcgggt
     1921 gacggggctg tcactgtcgg cgccgcggca agtatgggcc gtctcgcggc tgatgctggt
     1981 gctgacgaac gtcgtgccgc tggcaatcgc tttgtacttg ctcgcccgcc tgatcgacga
     2041 gctgggcgcg accgattggg ggcggctgtt cactctggcc tgtgccgccc tggcgacatt
     2101 catcaccacc ttcagcagca cgctcaacaa ccattcgctg gccgcctgca cgctcgtctt
     2161 tgcgctggcg gccgccgtgc ccatctggca agggcgcggc cgctggtggc atttcgctct
     2221 agccgggctg tttttcgcct tcaccgcggc caatgaattg ccggccctgt cgatgctggt
     2281 gctcgcagcc gcggctttcg gctggcgatt tccggcgaaa accgcaatcg ccttcgctcc
     2341 ccctgccctg cttgtcgccg cggccgcctt ggggacgaat tacgacgccc acggcgactg
     2401 gcgcacgccg tacgcccatc ggcaggatgg cgccacgatc gg
//
LOCUS       DASUGB010000184         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1132142, whole genome shotgun sequence.
ACCESSION   DASUGB010000184 DASUGB010000000
VERSION     DASUGB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132142"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFV87_03635"
     CDS             <1..666
                     /locus_tag="VFV87_03635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HEX5102874.1"
                     /translation="LVGRWADKWYLNAVGWRGIRQRKVHLEMLLRKAIERVRAGGRPV
                     RLLDIATGCGRYVLETIASLPPGTVESAVLRDITTANLEAGRKLAEKLGLSQVKFEPG
                     DAFDEQSIAAIQPAPTIAIVSGLYELFPDNARVLASLRGIARAMRDGGLLIYTGQPWH
                     PQIEMIARVLPNRDGQPWIMRRRTQAEMDDLVRAAGFEKLEMEIDRWGIFTVSLAKTG
                     DLP"
     gene            663..1649
                     /locus_tag="VFV87_03640"
     CDS             663..1649
                     /locus_tag="VFV87_03640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102875.1"
                     /translation="MNDPSEILAGYFQLMNLNGAAHVYHEAVRCGLLEALRSGPKSAE
                     ELAASRSLALRPVRLLLPALAALGVVQARGEQFALSPLAELLVSSSYRNLGDEYWAHL
                     PELLKTDKPIVRMDDTAKSEAFYQAQAAILGWMLTPAAECAAQALVAEGVADGCAILD
                     LGAGSAVWSLTLARELPGATVTAVDWPAVLEVAAETAQNLGLPDRLTTIAGNYHEVAL
                     PASKFDLAILANVTHLETPDGNQALFAKARAALKAGGRIAIFDVFPGQPAGDLNRTLY
                     ALGLALRTGHGHVYSVAEFEPLLAAAGFEPPKLVPLAVPPYAVGMLTAISRQ"
     gene            1996..2736
                     /locus_tag="VFV87_03645"
     CDS             1996..2736
                     /locus_tag="VFV87_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102876.1"
                     /translation="MKRIVGWFRKAENLNAILHSLVGSIVFAVVLSLGAMGWSYVQGL
                     SPPWIAITGVAALGSVLWGLRQVLAMFPEPPAKPDDGIEQSREGREDEPLVSPEAAGG
                     ALFGNHASTLREEMLQNQIAKLNSQLQSARKRALDAEKAVEAAQGRHAKEQSIADQRR
                     ERLLETKAAIREQINAAPPDFEYDSVKAWEESTNDLLRRMGLSRQLPQRVLNLKYDAG
                     PNRQRFQQNIAYLRGLIVTLTERDIPKH"
     gene            3965..>5071
                     /locus_tag="VFV87_03650"
     CDS             3965..>5071
                     /locus_tag="VFV87_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102877.1"
                     /translation="MARISRRTAIQYLAATGIGGAAAAAWAQNDQKKARPKAAKKAKP
                     AVPEISFPPELPGGKPSVTDTSDEFLKAPESLREGVAIAKTPPTIDFAYFPGQDYEGK
                     PWSNWGDATAIAGKYYTAIGDHLAPAGNACVFEYDAAKKSFRKLLDLKSLLHQPEGHY
                     SPAKFHSRVDLGSDGWLYCSTHRGSPRVTNDTYHYTGDWIVRCHPESGAAEIVVHAPV
                     PKHCIPNGTLDPDRLIFYGGTAPGEGEDGEGVMFFAYDCKNRKLLYSGPDGPARYMIL
                     ARSTGRVYYVPGKGDSPLMRFDPATDKAPVEIPGEIGIRAATRETPQGIVYTASLGQG
                     AGAEATIYAFNTKTEKIENLGPAAAGSQQYIAAMT"
BASE COUNT          992 a         1490 c         1683 g          906 t
ORIGIN      
        1 ctcgttggcc gctgggccga caagtggtac ttgaatgccg tcggctggcg ggggattcgc
       61 cagcggaagg ttcacctgga gatgctgctg cggaaggcga tcgagcgggt tcgcgccggc
      121 ggccggccgg tgcgactgct cgacatcgcc accggctgcg ggcggtatgt gctggaaacg
      181 atcgcatcgc tgccgccggg aacggtcgag tcggccgtgc tgcgcgacat cacgacggcc
      241 aacctcgagg cgggacgaaa actggccgag aagctggggc tctcgcaggt gaagttcgag
      301 cccggcgacg ccttcgacga gcagtcgatc gcggcgattc agcccgcgcc gaccatcgcg
      361 atcgtctccg gcctgtacga gctgttcccc gacaacgccc gcgtgctcgc ttcgctccgc
      421 ggcatcgcgc gggcgatgcg cgatggcggc ctgctgatct ataccggtca gccgtggcat
      481 ccgcagattg agatgatcgc ccgcgtgctg cccaatcgcg acggccagcc gtggatcatg
      541 cggcggcgga cgcaagccga gatggacgac ctggtccgcg ccgccggctt cgagaagctg
      601 gagatggaga tcgaccgctg gggaatcttc acggtctcgc tcgccaagac gggcgatctg
      661 ccatgaacga ccccagcgag atccttgccg gctacttcca gttgatgaat ctcaacgggg
      721 cggcgcacgt ttatcacgag gcggtgcggt gcggcctgct ggaagcgctc cgcagcgggc
      781 cgaagtctgc agaagaactg gctgcgagcc gcagcctcgc cttgcggccc gtgcgattgc
      841 tgctgccagc gttggcggcg ctcggagtcg tgcaggcccg cggcgagcag ttcgcgctca
      901 gcccgctggc cgagttgctc gtgtccagca gttaccgcaa cctgggtgac gaatactggg
      961 cgcatctgcc ggagctgctg aagaccgaca agcccatcgt caggatggac gacaccgcca
     1021 agagcgaagc cttttatcag gctcaggcgg cgattctcgg ctggatgctc acgccggcag
     1081 ccgagtgcgc cgcccaggcg ctggttgcgg agggcgtggc cgacggctgt gcgattctcg
     1141 atctcggcgc gggttcggcg gtctggagcc tgacgctggc tcgcgaactt cccggagcga
     1201 cggtgaccgc cgtcgattgg cccgccgtcc tggaagtcgc cgccgagacg gcgcagaacc
     1261 tggggctgcc cgatcggctg acgacgatcg ccggcaacta ccacgaggtc gcgctgccgg
     1321 catcaaaatt cgacctggca atcctggcca acgtgacgca cctcgaaacg cctgatggaa
     1381 accaggcgct cttcgccaag gcccgcgccg cgctgaaggc tggcggacgc atcgccatct
     1441 tcgacgtctt tcccggccag ccggcgggcg acctgaaccg cacgctgtat gccctgggat
     1501 tggcgctccg cacggggcat gggcacgtct attcagtcgc ggagtttgag ccgctcctgg
     1561 cggctgccgg atttgagccg ccgaagctcg ttccgctggc cgtgccgcct tatgccgtcg
     1621 ggatgctgac agcgattagc cggcaataat cgcggccggc gcatttgttt aacgagcagc
     1681 gccggcgagt ggcatccagc cgcagcgccg catgaacgtg cgaatggtca gattgttccg
     1741 ctcaacgtgg ctgattcaac ttctgcgggc ggccgggcga aatgcgcgtt acgccggcga
     1801 tggccgctgg cgtgacaaat aggctgtgga ggtttgacga tcttctggct ggatggccga
     1861 ctgcctttcg ctaaaatgac gaacgccgtc cagttcgagc tggcggcgtt cgattatttg
     1921 aggccgggag gccgtgcgaa ttgcgtactc gcatgatggc gcttcggtgc gcctagtcaa
     1981 tccgggcttg ccaccatgaa gagaatcgtc ggatggttcc gcaaagcaga aaacctaaat
     2041 gcgattttgc acagcttggt tgggagcata gtattcgcag ttgtattgag tcttggtgct
     2101 atgggctggt catacgtgca aggattgtcc cctccctgga tcgcgattac tggcgttgcg
     2161 gcgctggggt cagttctctg ggggcttcgc caagtcttgg cgatgtttcc agagcctccc
     2221 gccaagcctg acgatggtat tgagcagtca cgagaggggc gggaggatga gccactcgtt
     2281 tcgccggagg cggctggagg ggctttgttt ggcaatcacg catcaactct tcgagaggag
     2341 atgctccaga accagattgc aaaactgaac tcgcaactcc aaagtgcccg taagagagcg
     2401 cttgacgccg agaaggcggt tgaggcggcg caggggcgac acgccaagga acaaagcatc
     2461 gccgaccagc gccgcgaaag gcttttagag accaaagcag ccatccgcga gcaaatcaat
     2521 gccgcgccgc cagattttga gtacgactca gtcaaggctt gggaggaatc tacaaacgat
     2581 cttctgcgcc gcatgggcct gtcgaggcaa ttgccgcaac gagttcttaa ccttaagtat
     2641 gacgcagggc cgaaccgcca gcgatttcag cagaacatcg catatctgcg cggtttgata
     2701 gttactctga cggaacgcga catcccaaag cattagacgg ctgcccactt cttaggcagc
     2761 caagaaaatc aaaaagcatc actacctttt tcctgatccc cggcgtccga atactatgtg
     2821 atgccgctca ggggaactgg caccaggtga acttccgctt gccgtgcagc aggggcgttc
     2881 acgccccttc ggccgctgag gcccggcgtt tacgccgggt cgcgacggaa acgccattcg
     2941 ccgtgtccag ccccgttcac ggggcttctg caatctggct ttagctcaag ccgcaattgg
     3001 gaagccccat gaatggggct gacgcagagg gatcgcgccg tcgcatcacc cgccgtgaac
     3061 ggcgggtcta aacgagaaag ccccgtgaac ggggctgcgg caactccggc ccagcgtgtg
     3121 attgagcggt agccagtttc gggtcgatgc ctttcgcccc agccgtctcg aatgtggggt
     3181 agtcagaatc cgtgagaaaa tttcggcacg ggggaaataa tcggaggcgt ttgcgcatct
     3241 atacagtatg aaagtggtcg ggtgtgatcg tgtttggcaa ggtgggggac gtgggtagtt
     3301 gcggctgtga cgggggggag ggcattattc aggacgtcaa taaacgccgg acgttttttt
     3361 gcgggagtga atgaatgagg gtcagtggtc aggagccagg agccaggagt gaggagccag
     3421 gagccaggag ccaggacagg agacaaaaaa gcgcaacagc gcaacatcct cgaatggggg
     3481 aggcgatgcg gtgcaagaca ttgattggcg aagagttgcg gcaaatgttg cgcgggagaa
     3541 gttgcgcaaa aaaaatgcgg agagttgcgc attggcgcgg gaagcgatcc ggctggagag
     3601 gctgcagagg acagaaagat gaatgacagg aaaatgcccg cgattcccag tttcttgtcg
     3661 tcgatcttcc tgtcatgacg ggcgaatagg agccagaggc tgaaaatgcg caacagcgca
     3721 acatcctcga atgggggagg cgatgcggtg caagatattg attggcaagg acttgcggcg
     3781 aatgttgcgc gggagaggtt gcgcaaaaaa aatgcggaaa gttgcgcgct gggccctttg
     3841 ccgtcggcat gcggtcggcc gcggctctca ataatcgggg gcggcggagc atctgtttga
     3901 cggtccgccg cccaccggcg aagatggtac gcatccgctg gaactctttg caaggaaaga
     3961 cattatggca cgcatttcgc gccgcacggc cattcaatat ctggctgcga ccggcattgg
     4021 cggggctgcc gccgctgcct gggcgcagaa cgaccagaaa aaggccagac ccaaggcggc
     4081 caagaaggcc aagccggccg tgcccgagat ttcgtttccg cccgagctgc cggggggcaa
     4141 gccctcggtc accgatactt cggacgagtt cctgaaggcg cccgagtcgc tccgcgaagg
     4201 cgtcgccatc gccaagacgc cgccgacgat cgactttgcc tatttcccag ggcaggatta
     4261 cgaggggaag ccgtggtcga attggggtga cgcgaccgcc attgccggca agtattacac
     4321 ggcgatcggc gaccatctcg cgcccgccgg caatgcgtgc gttttcgagt acgacgcggc
     4381 gaaaaaatcg ttccgcaagc tgctcgacct caagtcgctc ctccatcagc cggaagggca
     4441 ctactcgccc gccaagttcc acagccgcgt cgatctggga agcgacggct ggctgtattg
     4501 ttcgacgcac cgcggctcgc cgcgtgtgac aaacgacacg tatcactaca cgggcgattg
     4561 gatcgtccgc tgccatccgg agagcggagc ggccgagatc gttgtgcatg ctcccgttcc
     4621 caagcactgc attcccaatg gcacgctcga tcccgaccgg ctgatcttct acggcggcac
     4681 cgcgccgggg gagggggagg atggcgaagg cgtgatgttc tttgcctacg actgcaagaa
     4741 ccgcaagctg ctgtatagcg gaccggatgg cccggcgcgg tacatgatcc tggcccgctc
     4801 gaccggccgc gtgtactacg tgcccggcaa gggcgattcg ccactgatgc ggttcgatcc
     4861 ggcgaccgat aaagcgccgg tcgagattcc cggcgagatc ggcatccgcg ccgccacgcg
     4921 cgaaacgccg caggggatcg tctatacggc ttcgctcggc caaggcgccg gcgcggaggc
     4981 gacgatttac gccttcaaca ccaagaccga gaagatcgag aacctcggac ccgccgccgc
     5041 cggctcgcag cagtatatcg ccgcgatgac t
//
LOCUS       DASUGB010000185         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_993980, whole genome shotgun sequence.
ACCESSION   DASUGB010000185 DASUGB010000000
VERSION     DASUGB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993980"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFV87_03655"
     CDS             complement(<1..380)
                     /locus_tag="VFV87_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102878.1"
                     /translation="MTDIELKTVRDLSPGEWEIWDDIRAANPVYASPYFAPEFAQAVA
                     SVRDDVEVAVLKQGSATVGFFPFQRGRLNLGKPLGGKLSDYHGPLAGTGAMIDPRSLL
                     RSCGLAAWDFDHLVGPTELFEPFVT"
     gene            complement(377..1447)
                     /locus_tag="VFV87_03660"
     CDS             complement(377..1447)
                     /locus_tag="VFV87_03660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5102879.1"
                     /translation="MKIESIQICDLSPDLIARWENILNQSPELASPYFRPEYVQTIAG
                     VRSGVEVGVLRDGNDVLGFWPFERAPGAVAKLVGSRLSDYQAVIAAADTPWTVELILR
                     GCRLKAWEFDHQLASQTQLAPYFTSTGESRHLDLSPGYEQYVEQRKAAAKTLSETLRK
                     FRKFQREHDVRFVWHSDDEGAFQQLLQWKSEQYVRTGLDDLFAVGWIVELIRTIWRTQ
                     QPQFAGVLSVMYANDKIAAVHFSMQSGKRLHSWFPAYDVGLSKFSPGACQLLFMAQCA
                     AEHGIDCIDLGKGDEEYKLMFASGSVPLAEGVVETRPLSAAMRRGWRQTRDWVKQSPL
                     ERPARASLRWLRQVKQWIGSQP"
     gene            complement(1444..2430)
                     /locus_tag="VFV87_03665"
     CDS             complement(1444..2430)
                     /locus_tag="VFV87_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5102880.1"
                     /translation="MTTPAISIVVCTFNRAAMLRDALASLYSLATDDSFRYEIVVIDN
                     ASTDATPLAIAAAAAESPAPVRGIFEPQKGVVAARNRGIREARGRWIAFFDDDQLADP
                     RWLAELWRGAHDNSCRVVGGAVHLVLPMGCTRRLDPTVRMLLGESILSDQPLPYGGRL
                     TPGCGNLMIERTVFDEVGLFETAIDGRGEDTDLFVRIERAGIAAWYFPQAIIHHLTPP
                     ERLEPPYLLNLARKMGRGIGLRQARAMSRGAFTALWLAKAVRAGLLQWPVLALSHLRS
                     DREAALGRACQLAISRGFLSGGCEAGRPPPTAGDNPIRTTLSSPVLSGANSK"
     gene            complement(2434..3762)
                     /locus_tag="VFV87_03670"
     CDS             complement(2434..3762)
                     /locus_tag="VFV87_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5102881.1"
                     /translation="MSTAATNLPLSLSPPLPLSGEVPRWPLLLTALVIGLVIFVTEHD
                     LSISQADAFTQTADEMEVTAEGGNFVRRLAFPSLALLGLVLAYSTKRQPWRINLLLAL
                     PMGSYLALAATSYLWSYDPGMTMRRLIVVGCCTVAGVGIARRFSMHELCLLTLAIVGP
                     LTLLGLAAELRLGTFRPWSGDYRFSGSVHPNTQTSYLTALALAALGLARSGSRWKVSL
                     WTIFAASLVLMVLTKSRTGNAATLAAIASVFLVQTSLRFKFSAGLAAAWIGLGGLWLL
                     LIAGYDPLVDFHQALLLGRAEESDTLSGRSFIWPAVGYFIDQRPWLGYGFEAFWNPTH
                     VETISEEVGWGLREAHNGYLEMLLSLGIVGLGLALATVAAGVAAAVRGGVKLRDPAYS
                     LPLGMLVYGVLVSAMESGMVIVEFATFLTGVCLLRMALFEKPVPQVTHDK"
     gene            complement(3759..>5119)
                     /locus_tag="VFV87_03675"
     CDS             complement(3759..>5119)
                     /locus_tag="VFV87_03675"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX5102882.1"
                     /translation="SSASLPVAGSGASGLTFPSKAWERGAAAARYICRLASSPLLQKS
                     ALSVFDQAVVSGTSFVTAVILARQCPREELGVYYLALSLIFFVRGIQEQIVCAPYMIY
                     CSRKQGTALAEYAGSSLIHQCVVMSAASTILAGALLLGFAPAVTQAAFWLLVGAGPLL
                     LLREYIRQMSFAHLEMRTAITVDVAVSVLQLVAMGGLVLTGHLGVVETLATMGISSGV
                     PAIVWLLAKSRPMLPRLDAALRDFLSNWTFARWALASQLLACTTPYVMPWVVALTHGE
                     ADTGTLGACVTLVGLANMFMMGLCNFLSPRAARAFAEGGLAELKSVLAKTALLFGLSL
                     GSIAVAGFLVGEPVAMLVYGPQFAGTGWIIGVLSLSVLANSMGVTAGNGLWAMERPQA
                     NFVADLTSLGVVIVATISLVPLFGPLGAALATLSGTTTDAAVRLLILRLTMRELAAGR
                     TP"
BASE COUNT         1048 a         1616 c         1630 g          825 t
ORIGIN      
        1 gtgacgaagg gctcaaacag ttccgttggg cccacgagat ggtcgaaatc ccaggccgcc
       61 agaccgcagc tccgaagcag cgaacgagga tcgatcattg ctccggtacc agcgagcgga
      121 ccgtgataat ccgacagctt cccccccagc ggcttgccga ggttgagtcg tccgcgctgg
      181 aaggggaaga atcccactgt ggccgagccc tgcttcaaaa cggcgacctc cacgtcgtcg
      241 cgcacagaag caacggcctg ggcgaattca ggtgcgaaat acgggctcgc gtacacggga
      301 ttcgctgccc ggatgtcgtc ccaaatctcc cattcgccag gcgaaagatc gcggacggtt
      361 ttgagttcaa tgtctgtcac ggctggctcc cgatccattg tttgacctgt cgcaaccacc
      421 tgagcgaagc gcgggccggc ctttccagcg gcgattgctt gacccaatcg cgcgtctgcc
      481 gccagccgcg gcgcattgcg gccgagaggg gccgcgtttc gaccacgcct tccgccagtg
      541 gcacgctgcc gctggcgaac atgagtttgt attcctcgtc gcctttgccc aggtcgatgc
      601 agtcgattcc gtgctcggcg gcacactggg ccatgaagag caactggcag gcccccggcg
      661 agaactttga cagccctacg tcgtaggcgg gaaaccacga atgcagccgt ttcccgctct
      721 gcatgctgaa atggacggcg gcgatcttgt cgttggcgta catcacgctc agcacgcctg
      781 cgaactgcgg ctgttgcgtc cgccagatcg tgcggatcag ctccacgatc caaccaaccg
      841 cgaacaagtc atccaggccg gtgcgaacgt actgctcgga tttccattgc agaagttgct
      901 ggaaagcccc ttcgtcatcc gaatgccaga caaatcgcac gtcgtgctcg cgttggaatt
      961 tgcggaactt gcggagcgtt tcgctcagcg ttttggcggc ggctttgcgc tgctcgacgt
     1021 actgctcata accgggtgac agatcgaggt gccgggattc gccggtggaa gtaaagtacg
     1081 gcgccagttg ggtctgactc gcaagctgat ggtcaaattc ccaggccttc aagcggcagc
     1141 cgcgaaggat gagctcgacg gtccagggcg tgtcggcggc ggcaatcacg gcctggtaat
     1201 ccgacagccg actaccaacg agctttgcga ccgcacctgg agctcgttca aagggccaga
     1261 agccaagcac atcgttgccg tcgcgcaaga cgccgacttc gacgccgctg cgcacgcccg
     1321 caatggtctg cacgtactcg ggccggaagt acgggctggc cagctcaggc gactgattca
     1381 gaatgttttc ccagcgcgcg atcagatcgg gggataaatc gcagatttgg atggactcga
     1441 ttttcatttg gagttcgctc ccgacagaac gggactggaa agggttgttc ggattggatt
     1501 gtcacccgcg gtcggtggcg gcctgccggc ttcgcaccca ccgctcaaaa acccgcgact
     1561 gatcgccagt tgacaagcgc ggccaagtgc cgcttctcga tcgctgcgaa ggtggctcag
     1621 cgccagcacg ggccattgca gcaatccggc gcgaaccgct ttggccagcc acagcgccgt
     1681 gaaagctccg cggctcatgg cccgcgcttg tcgtaagccg atgccgcggc ccattttacg
     1741 ggcgaggttg agcaaatacg ggggttcaag tcgctcgggc ggcgtcaggt ggtggatgat
     1801 tgcctgcggg aaataccagg cagcgatgcc ggcgcgctcg attcggacaa acaggtcggt
     1861 atcctcgccg cggccatcga tggccgtctc gaacaggccc acttcgtcga agaccgtccg
     1921 ctcgatcatc aggttgccgc agccgggcgt gaggcggccg ccatagggca gcggctggtc
     1981 ggaaagaatc gattcgccga ggagcatgcg gaccgtcgga tcgagccgcc gagtgcagcc
     2041 catgggcagc acgaggtgga ccgccccgcc cacgacgcgg cagctgttgt catgagcgcc
     2101 acgccacagt tcggcgagcc agcgcggatc agcaagttga tcgtcgtcga agaaggcgat
     2161 ccaccgccca cgggcctcgc gaatgccccg attgcgggcc gccacgactc ctttctgcgg
     2221 ctcaaagata ccgcggacag gcgccggcga ctcagccgct gcagcggcga tggccagggg
     2281 cgttgcatcg gtagaagcat tgtcgatgac gacgatctcg tagcgaaacg agtcatcggt
     2341 cgccaatgag taaagactcg ccagcgcgtc gcgcagcatg gcggcgcggt tgaatgtgca
     2401 gacgacaatc gatatcgcgg gggttgtcat gggtcacttg tcatgggtca cttgtggaac
     2461 cggcttttca aagagggcca ttcgcaacaa gcagacgccg gttagaaacg tggcgaattc
     2521 gacgattacc attccggact ccatcgccga gacgagcacg ccataaacga gcattcccag
     2581 cggcagggaa taggcgggat cgcggagttt cacgccgcct cgcacggccg cggcgactcc
     2641 cgccgcgacg gtggccagcg ccaatccgag gccgacaatt cccagcgaca ggagcatttc
     2701 gagatacccg ttgtgggcct cccgcagccc ccagccgacc tcctccgaaa tcgtctcgac
     2761 gtgcgttgga ttccagaacg cctcgaagcc atagccgagc cacggtcgtt gatcgatgaa
     2821 gtagccgacg gccggccaga tgaacgaccg tccgctgagg gtgtcggatt cctcggcacg
     2881 ccccagcagc aaggcctggt ggaaatcgac cagcgggtcg tagccggcaa tcaatagtag
     2941 ccagaggccg ccgagtccga tccacgctgc ggccagtccg gcggaaaact tgaatcgcaa
     3001 cgaagtctgc accagaaaca cactggcgat ggccgcgagc gtcgcggcat tgccggtgcg
     3061 cgacttggtg agcaccatca gcacgagcga cgccgcgaag atggtccaca gactgacttt
     3121 ccagcgactg ccggagcgcg ccaggccgag cgcggccagc gccagcgcgg ttaaatacga
     3181 cgtttgcgta ttcggatgga ccgagcccga gaagcgataa tctcccgacc acggcctgaa
     3241 ggtcccgagg cgaagttctg cagccaggcc gaggagcgta agcggtccca cgatggcaag
     3301 cgtcaacagg cacagttcgt gcatcgagaa ccgccgcgca atgcccacac cggcgaccgt
     3361 gcagcagccg accacaatca gccggcgcat ggtcatgccc ggatcgtacg accagagata
     3421 gctggtcgcc gccagtgcca ggtacgatcc catcggcagg gccagcagca ggttgattcg
     3481 ccagggctgg cgtttcgtgc tgtaggccag cacgagtccc agcaaggcca acgacggaaa
     3541 cgccagcctc cgaacgaaat tgcccccctc cgcggtgacc tccatttcgt cagccgtctg
     3601 cgtaaacgca tcagcctgag aaatgctcag gtcgtgttcg gtgacgaaga tgaccaggcc
     3661 gatgacgagg gccgtgagca acagcggcca gcgcgggact tcgcctgaga gggggagagg
     3721 gggagagagg gagagaggga gattcgtggc ggctgtgctc atggcgtcct ccccgcggct
     3781 agttcgcgca tcgtcaggcg gaggatgagc aggcggacgg cggcatcggt cgtagttccc
     3841 gagagtgtcg ccagcgccgc gccaagcggc ccaaatagcg gcacaaggct gatcgtcgcg
     3901 acgattacca cgcccagcga ggtcaggtcg gcgacgaaat tcgcttgcgg acgttccatc
     3961 gcccagaggc cgttccctgc cgtcacgccc atgctgttgg ccagcacgct gagcgacaga
     4021 acgccgatga tccagccggt gccggcgaac tgcggtccat agacgagcat cgccacgggt
     4081 tcgccgacca ggaaccccgc tacagcaatg ctgccgagcg agagtccaaa gagtagcgcc
     4141 gttttcgcca gaacggattt cagctccgcc agtccgcctt cggcaaaagc tcgggccgcg
     4201 cgcgggctga ggaagttgca cagccccatc atgaacatgt tggcgaggcc gacgagcgtc
     4261 acgcaggcgc cgagcgtgcc ggtgtcggct tcgccatggg tcaaagccac gacccagggc
     4321 atcacatacg gcgtcgtgca ggccaagagc tgactggcca gcgcccagcg ggcaaacgtc
     4381 caattgctga gaaagtcgcg cagcgctgcg tcgagccgcg gcagcatcgg gcggctcttg
     4441 gccagcagcc agacgatggc cggaacgccg ctggaaatgc ccatcgtggc cagcgtctca
     4501 acgacgccca agtggcccgt caacaccagg ccgcccatcg ccacaagctg caggacgctg
     4561 acggcgacat cgacggtaat cgccgtccgc atctccaagt gagcgaacga catctggcgg
     4621 atgtactcgc gcagcaagag gagcggccca gcgccgacga gcagccagaa tgccgcttga
     4681 gtcaccgccg gggcaaatcc caaaagcaac gccccggcga ggatcgtgct ggccgcggac
     4741 atcaccacac attgatgaat caaggagctg ccggcatatt ccgccagggc cgttccttgc
     4801 ttgcggctgc aatagatcat gtacggggcg cacacgatct gctcctgaat gccgcggacg
     4861 aaaaagatca ggctgagagc caggtaatac acgcccaact cttcgcgcgg gcattggcga
     4921 gccaggatca cggcggtgac gaagctcgtc ccgctgacga ccgcctggtc gaataccgac
     4981 aaggcacttt tttgcaagag cgggctggag gccagccggc aaatatagcg cgcggccgcg
     5041 gctcctcgtt cccaagcttt gcttgggaac gtgagtccgg aagctccgct tccagccaca
     5101 ggaagggaag cagagctcc
//
LOCUS       DASUGB010000186         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_855358, whole genome shotgun sequence.
ACCESSION   DASUGB010000186 DASUGB010000000
VERSION     DASUGB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855358"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFV87_03680"
     CDS             <1..1204
                     /locus_tag="VFV87_03680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX5102883.1"
                     /translation="DMPTEYKDQYEVLIVGGSTAGITVAAQLRRKLRSYDLAIVEPAA
                     KHYYQPLWTLVGAGVFRKEWSERAQSAFIPPGADWIQESVAEFHPDENAVVTTAGRKI
                     GYEHLVVALGIQIDWGKIPGLKEGLGSNGLCSNYSYQHVDYTWDCIRNFRGGTALFTM
                     PNTAVKCGGAPQKIMYLAEDHFRRSGVRDESQVIFASAQGSLFAVEKYRKTLEKIVER
                     RGIETRFGHNLVELRPDAKEAIFEHLGTHERVVIPYDMAHVTPPMSAPDVIKQSPLAN
                     SGGWVDVDKFTLQHPRFANVFALGDCSGLPTSKTGAAIRKQAPVVVKNLLAAREQQPL
                     TATYDGYTSCPIVTGYGRLILAEFDYEGKPQETFPFDQSKERLSMYLLKAYALPRLYW
                     DGMLRGRA"
     gene            1246..>2011
                     /locus_tag="VFV87_03685"
     CDS             1246..>2011
                     /locus_tag="VFV87_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5102884.1"
                     /translation="MKLVIVGGVAGGASAAARARRLSEAAEIVVVERGPDVSFANCGL
                     PYYIGGEIQDRRKLLVVTPERLKERFNLDVRIRASAEAIDRSNRTVRIRELDTGREYD
                     EAYDRLILAPGAAPLRPPIPGIDLPGVFTLRNLQDMDRIKARIDSGAGKAVVVGAGFI
                     GLELVENFVRQGIATTVVELQDQVLPPLDKEMTTPLAEVLAAHGVNLLRGDSAEAFEA
                     GEEGVTVRLKSGQRLAADLVVLGIGVRPENKLAVDAG"
BASE COUNT          387 a          575 c          674 g          375 t
ORIGIN      
        1 ggacatgcca acggaatata aagaccagta cgaggttctg atcgtcggcg gcagcacggc
       61 cggcattacc gtcgccgcgc agctccggcg aaagctgcgt tcgtacgatc tggcgatcgt
      121 cgagccagcg gccaagcact actatcagcc gctctggacg ctcgtcggcg cgggcgtttt
      181 ccgcaaagag tggagcgagc gggcccagtc cgcctttatt ccgccgggag ctgactggat
      241 tcaggaatcg gttgcggagt ttcatcctga cgagaatgcg gtcgtgacca cggccggccg
      301 gaagatcggc tatgaacacc tggttgtggc gcttggcata caaatcgact ggggcaagat
      361 tccgggcctc aaggaaggtc tgggcagtaa cggactctgc agcaattact cgtatcagca
      421 cgtcgactac acctgggact gcattcgcaa cttccgcggt gggacggcgc tctttacgat
      481 gccaaacacg gccgtcaaat gcggcggggc gccgcagaag atcatgtatt tagcagagga
      541 ccacttccgg cgcagcggcg tgcgcgacga gtcccaagtg atttttgcct cggcacaggg
      601 ttcgttgttc gcggtcgaaa agtaccgcaa gacactcgaa aagatcgtcg agcgccgcgg
      661 tatcgagacg cgcttcgggc acaatctcgt cgagttgcga cccgacgcta aggaagccat
      721 cttcgagcac ttgggcacgc acgagcgcgt cgttattcct tacgacatgg cccacgtcac
      781 gccgccgatg agcgcgccgg acgtgatcaa gcagagccct ttggccaata gcggcggctg
      841 ggtagacgtc gataagttca cgctccagca tccgcggttt gcgaacgtct tcgccctggg
      901 cgactgcagc ggcttgccga catccaaaac cggcgccgca atccgcaagc aggcgccggt
      961 cgtggtaaaa aatctgctgg cagccagaga gcaacagccg ctgacggcaa catacgacgg
     1021 ctatacgtcg tgcccgattg tgacgggata tggccggctg attctggccg agtttgacta
     1081 cgaaggaaaa ccccaggaga cttttccgtt cgatcagtcg aaagagcgcc tgagcatgta
     1141 cttgctcaag gcctacgcct tgcctcgact gtactgggac gggatgctac gcggccgggc
     1201 gtagcgcttg cgttctgcga cactctggtg cggaagggat tcgccatgaa actggttatc
     1261 gtcggcggcg tggcgggcgg ggcttcggcg gcggctcggg cgcggcggct gtcggaagcg
     1321 gccgagatcg tggtcgtgga gcgcggaccg gacgtttcgt tcgccaactg cggactgccc
     1381 tactacatcg gcggtgagat ccaggaccgc aggaagctgc tggtggtcac gcccgagcgg
     1441 ctgaaggaac ggttcaacct cgacgtgagg attcgcgcgt cggccgaggc gatcgatcgg
     1501 tcgaacagaa ctgtgcggat tcgcgagctg gatacgggcc gcgagtacga cgaagcgtat
     1561 gacaggctga tccttgcgcc gggtgctgca cccttgcggc cgccgattcc cggaatcgat
     1621 ctgccaggtg tctttacgct gcggaatttg caggacatgg atcgtattaa ggcccgcatc
     1681 gactcgggcg ctggtaaggc ggttgtcgtc ggtgcgggat ttatcggtct ggaactggtt
     1741 gaaaacttcg ttcgccaagg gatcgcgacg accgttgtcg aattgcagga tcaggtcctg
     1801 ccgccgctcg ataaggaaat gaccacgccg ctggcggaag tgcttgcggc gcatggcgta
     1861 aacctgctcc ggggcgattc ggccgaagcg ttcgaggccg gcgaggaggg ggttacggtg
     1921 cggctcaagt cgggccagcg cctggcggcc gatctggtcg tcctgggcat cggcgttcgc
     1981 ccggagaaca agctggcagt cgatgccggc c
//
LOCUS       DASUGB010000187         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_298753, whole genome shotgun sequence.
ACCESSION   DASUGB010000187 DASUGB010000000
VERSION     DASUGB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298753"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3021
                     /locus_tag="VFV87_03690"
     CDS             <1..>3021
                     /locus_tag="VFV87_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEX5102885.1"
                     /translation="VLDPIVSIRQTVLLTPNQSVRIDLVTGVAESRAAVEALADKYRD
                     ASLADRVFDIAWTHSHVLLQQLGASEADAQVYGRLVGSIIYASGLRRAKASVLAHNRR
                     GQSGLWGYGISGDMPIVLVRIRDHERIGLVRQAVLAHAYWRLKGLSVDLVIWNEDDSV
                     YRQTLQEAIIDLVAASPEASLVDRPGGIFIRRGEQMSDEDRALMQTVARIVLLDDGGT
                     LAEQAERRGRADVSIPQLKPIRRQPEPPVPYEPPRHDLAFFNGLGGFSHDGREYVTIL
                     SPGRTTPAPWVNVIANPQFGTVVSEGGSAYTWAENSREFRLTPWRNDPVSDLGGEALY
                     LRDEESGHFWSPTPQPTRGKNTYVVRHGFGYTIFDYTEEGLTTELCVYVATDSPVKIY
                     KLKIANHSGRTRQLSIIGYWELVLGDFRSKTLLHVVTELDPASGAILARNVYSPEFGD
                     RVAFVNCSESSRTFTGDRAEFLGRNGELANPSAMRRVRLSGRVGAGYDPCAAFHAPLT
                     LAEGHERTITFTIGAAVGAAEARSLAQRFRSVDSAHRAIEGVWDYWSRTLGVVYLETP
                     DATVNFLANGWLVYQTLACRMWARTGFYQSGGAFGFRDQLQDAMALVHAQPSLLREHL
                     LRAAGRQFRAGDVQHWWHPPLGRGVRTHISDDFLWLPLAICRYVNITGDTGVLEQRIP
                     YLTARPLRDDEESNYDLPQVSDEVGTLYEHGVRALDHGLRFGEHGLPLMGGGDWNDGM
                     NLVGQHGRGESVWLAFFLFQVLTQFADLARRRGDVALADRYAVEAGRLRGNIEASGWD
                     GQWYRRAYFDDGTPLGSAANEECQIDAISQSWSVLSGAGTHERTIEALASVDRRLVRR
                     DPRLILLLDPPFDKSNLNPGYIKGYVPGVRENGGQYTHSAIWTVMAMAAMGDRERTWE
                     LFGLINPISHGATPDDVSIYRVEPYVVAADVYGVDPHKGRGGWTWYTGSAGWMYRLIV
                     ESLLGLHLQVDKLRFAPCLPDDWTEFKIHYRYRET"
BASE COUNT          507 a          977 c         1017 g          520 t
ORIGIN      
        1 gttctcgatc cgatcgtcag catccgacag accgtgctgc taacgccgaa ccagtcggtg
       61 cggatcgacc tggtcaccgg cgtcgccgaa tcgcgggccg cggtggaagc gctggccgac
      121 aaataccgcg acgccagcct ggccgaccgc gtcttcgaca tcgcttggac gcacagccat
      181 gtcctgcttc agcagctggg cgcttccgaa gcggatgcgc aggtctatgg ccgcctggtg
      241 ggctcgatca tctatgcctc ggggctgcgg cgggcgaagg cgagcgttct ggcccacaat
      301 cggcgcggcc agtcgggatt gtggggctac ggcatctcgg gggacatgcc gatcgtgctg
      361 gtgcggattc gcgaccacga gcggatcgga ctggtccggc aggccgtcct ggcgcacgcc
      421 tactggcggc tgaaggggct gagcgtggat ctggtgatct ggaacgaaga cgactcggtc
      481 tatcgccaga cattgcagga agcgatcatc gatcttgtcg ccgccagccc cgaagcgtcg
      541 ctcgtggacc gccccggcgg cattttcatt cgccgcggcg agcagatgtc cgacgaggac
      601 cgggcgctca tgcagaccgt ggcccggatc gtgctcctgg acgacggcgg gaccctggcg
      661 gaacaggccg aacgccgcgg gcgggcagac gtatcgatcc cgcaactcaa accgatccgc
      721 cgccagcccg agccgccggt cccctacgag ccgccgcggc acgacctggc ttttttcaac
      781 gggttgggcg gcttcagcca cgacggccgc gaatacgtga ccatcctctc gccgggtcgg
      841 acgacccccg ctccatgggt caatgtgatt gccaacccgc aattcggcac ggtcgtgtcg
      901 gaagggggaa gcgcctatac gtgggcggaa aacagccgcg agttccggct cacgccctgg
      961 cgcaacgatc ccgtgtcgga cctcgggggc gaggccctct atttgcgcga cgaagagtcg
     1021 gggcatttct ggtcccccac gccgcagccg actcgtggga aaaacaccta cgtcgtgcgc
     1081 cacggattcg gctacacgat tttcgattac accgaggaag gcctgacgac ggagctgtgc
     1141 gtctatgtgg cgaccgactc gccggtaaag atctacaagc tcaagatcgc caaccactcg
     1201 gggcgaacgc ggcagttgtc gatcatcggc tactgggaac tggtcctcgg cgattttcgg
     1261 agcaagacgc tgctgcacgt ggtcacggag ctggacccgg ccagcggcgc gattctcgcc
     1321 cgcaacgtgt acagcccgga gttcggcgac cgcgtggcgt tcgtcaattg cagcgaatcc
     1381 agccgcacgt tcaccggcga ccgcgccgag tttttgggcc gcaacggcga actggccaat
     1441 ccgtcggcca tgcgacgcgt gcggctctcg gggcgagtgg gcgcgggata cgatccgtgc
     1501 gctgccttcc acgcgccgct gacgctggcc gagggacacg agcggaccat caccttcacc
     1561 atcggcgcgg cggtcggggc ggcggaggcc aggtccttgg cgcagcgctt tcgcagcgtg
     1621 gacagcgccc accgggcgat cgagggcgtg tgggactact ggagccggac gctgggcgtc
     1681 gtctatctgg aaacgcccga tgccacggtc aatttcctgg ccaacggctg gctcgtgtat
     1741 caaacgctgg cctgccggat gtgggctcgc acgggcttct atcaatcggg cggcgcgttt
     1801 ggcttccgtg accagctcca ggatgcgatg gccctggtgc acgctcagcc gagtctgctg
     1861 cgggagcacc tgctgcgggc cgccggtcgc caattccgcg caggggacgt gcagcactgg
     1921 tggcatcctc cgctgggacg cggcgtgcga acgcacatct ccgacgattt tttgtggctc
     1981 ccgttggcca tttgccgcta tgtcaacatc accggggaca ccggcgtgct cgagcagcgc
     2041 atcccctatt tgaccgctcg tccgctccgc gacgacgagg aatccaacta cgacctgcca
     2101 caagtctccg atgaagtcgg cacgctctac gaacatggcg tccgtgcgct cgatcacggg
     2161 ctgcggtttg gagagcacgg tctgccgctg atgggcggcg gtgactggaa cgacggcatg
     2221 aacctggtgg ggcagcacgg gcggggcgaa agcgtgtggc tggcgttctt tctgttccaa
     2281 gtgctcacgc agtttgccga cctggcccgg cggcgcggcg acgtcgcact ggccgatcga
     2341 tatgcggtcg aagccgggcg gctgcgcggc aacatcgagg ccagcggctg ggacggccag
     2401 tggtatcgcc gggcgtattt cgacgacggc acgccgctgg gctccgcggc caacgaagag
     2461 tgccaaatcg atgcgatctc gcaaagctgg tcggttctct cgggcgccgg cacccacgag
     2521 cggacgatcg aggcgctggc cagcgtcgat cggcgcctcg ttcgccgcga tccgcgcttg
     2581 atcctgctgc tcgatccgcc cttcgacaaa tcgaacctga accccggtta catcaagggg
     2641 tacgtccccg gcgtccgcga aaatggcggc caatacacgc acagcgccat ctggaccgtg
     2701 atggcgatgg ccgcgatggg agaccgcgag cgaacgtggg agttattcgg gctcatcaat
     2761 ccgatttcgc acggggcgac cccggacgac gtttccatct accgcgtcga gccgtacgtg
     2821 gtggctgccg acgtctatgg agttgatccg cacaagggac gcggcggctg gacttggtac
     2881 accggatcgg ccggctggat gtaccgtctg atcgtagagt cgctcctcgg cctgcacctg
     2941 caagtggaca agctgcgctt cgcgccctgc ctgccggacg attggaccga attcaagatt
     3001 cactatcgct atcgcgagac g
//
LOCUS       DASUGB010000188         5636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1271335, whole genome shotgun sequence.
ACCESSION   DASUGB010000188 DASUGB010000000
VERSION     DASUGB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271335"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFV87_03695"
     CDS             <1..1181
                     /locus_tag="VFV87_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102886.1"
                     /translation="GLYDSLIVVIPSLDIVVARAGQSWKREKDAEHYEVLRPFLTPIA
                     RSPLSPRGRGVGGEGIGSPHAPREDRHHAERDAYHASPVIAGIDWAPPETIIRLAKGS
                     DNWPLTWGADDLLYTAYGDGRGFEPFVPKKLSLGLARISGSPPEIRGENILAPTVEAI
                     GDGKRARKASGMLMLEDGTLYMFVRNVANSQLGWSSDQGRTWEWADWKFETSFGCPTF
                     LNFGRANAGNRDEYAYVYSHDSDSAYERADRMALARVPQDLLREKAAYEFFVKRDDAG
                     AAVWTSEIARRGTVFENPRACYRSSVVYNAPLKRYLWCQTGAGDDTRFAGGFAIYDAP
                     EPWGPWTVAYRTEQWDVGPGESSGIPAKWISPDGLTFWLVFSGDDCFSVRKGMLRRKP
                     "
     gene            1178..2305
                     /gene="dgt"
                     /locus_tag="VFV87_03700"
     CDS             1178..2305
                     /gene="dgt"
                     /locus_tag="VFV87_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX5102887.1"
                     /translation="MTRPAISTTAEREAALLARYAMHSADSRGRKHQEAEHSYRGPFQ
                     RDRDRIVHSSAFRRLSGKMQVFTGERGDYHRTRLTHTHEVASIARTIGRALRLNEDLV
                     EALALFHDIGHPPFGHAGEDALDECLREHGGFSHNRYALTIAEQLEARYPAFPGLNLT
                     YEVLEGQATRADKAATYGSDVAPLLEVQVVEAADSMTYDAHDTDDAVKLRLVTIEELA
                     ANSLIAEALSLVRGGYTNLRPDLLRKAVVHQLIDRQVTDLLEATSPLLAQSASLNADE
                     VRRTSLRIAPSRELAEQKRELERFLYDRVYRHPELIAVRSHAQGRLREMFEGFVQKPD
                     LLPPWFQDRANAVGLPRAVGDYLAGMTDRFCDQQYELHFAP"
     gene            complement(2405..3403)
                     /locus_tag="VFV87_03705"
     CDS             complement(2405..3403)
                     /locus_tag="VFV87_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102888.1"
                     /translation="MTYLLGVDEAGYGPNMGPLVIGLSAWQVPERVGPEKLFARVSKI
                     ISQPGGEKARKSDDRLAIGDSKLLYKPAQRGASTAARSAPLRLLERGVRAILGLLDRR
                     PARWREIWSRVDPADANLQDAAPWHDGYDEQLPVDCGPEAEPDIVKLGEGLARADVRC
                     RELRARAIFPAEFNGLVERHGNKAEVLSLATLALVRGTLERLPNCPAIVTCDKHGGRD
                     YYAGLLQHVFPDELVKIRRETPELGVYQVQHAGRTVEFRFLVSGERMLPTALASMTAK
                     YLRELAMRPFNAFWQRHVPGLAPTAGYHTDSWRFLRDIRAVQERLQIDQRLFWRSR"
     gene            3519..4550
                     /locus_tag="VFV87_03710"
     CDS             3519..4550
                     /locus_tag="VFV87_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HEX5102889.1"
                     /translation="MQVSDPSPWRQAARLLAGAALGGMCVWAFAQEGGESEESQPRVP
                     FEPRAVEITFTDGSNLRLHLADEVIEMSSPHGKLLIPAEDVLRIEFAQRLPAETMRQI
                     GELLTQLKDTDEDMRANAGEGLLAFGPKAYLPVLKASKSGDPALAPIAGQVLQELRAQ
                     VPADELQVREDDLIETPDTKIAGRITMPSLKLKTSQFGELSMNLADARSLRHRSLLAT
                     APEPDEERGVLPDPGNLKAYEQKIGEVFEFSVTGAVGGATIWGTDTYTTDSSLAMAAV
                     HAGVLKDGETGVVKVKIVAGQPSYAGSAKNGVTSSGYGGYGGSYEFVKAAAAGRPGGR
                     FRGPRFLPK"
     gene            4690..>5636
                     /locus_tag="VFV87_03715"
     CDS             4690..>5636
                     /locus_tag="VFV87_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102890.1"
                     /translation="MPFVRSFVVALVVACTGRLIAAEAEWPQFRGPGGQGHAVAERLP
                     TTWSETENVAWKCPLPGKGWSSPVIAGNQIWMTTAVESPISDEEKARRLKETTNTQPL
                     VVSGSLSLRALCVDRTSGKLLHDVELLVEPRPQPTHAMNSFASPTPVIEDGKLYCHFG
                     AYGTACLDTATQKVVWSNRETVINHENGPGSSPILAGDVLIFHCDGSDQQYVAALDKQ
                     TGKLAWRTDRSGEMNAEPQLKKSYGTPLVVEIGGQPQVVSPGADWLYAYEPQSGRELW
                     KVPYEKLGFSIAPRPVAGHGMIFMSTAFMQPEILAVKYDG"
BASE COUNT         1018 a         1790 c         1870 g          958 t
ORIGIN      
        1 ggggactgta cgacagcctg atcgtcgtca tccccagtct ggatattgtg gtggcccgcg
       61 ccggccaatc gtggaaacgc gaaaaggatg ccgagcacta cgaggtcttg cggccgttct
      121 tgacgccgat tgctcgttct cccctctccc ctcgtgggag aggggtcgga ggtgagggga
      181 ttggtagtcc tcacgctccg cgtgaggaca ggcatcacgc ggagcgtgat gcctaccatg
      241 ccagccccgt catcgccggc atcgactggg cgccgccgga gacgatcatc cgcctcgcca
      301 agggaagcga caactggccg cttacctggg gcgccgacga cctgctctac acggcctacg
      361 gcgacggccg cggctttgag ccctttgtgc cgaagaaact cagcctgggc ctggcacgca
      421 tcagcggatc gccgccggag attcgcggcg agaacatcct ggccccgacc gttgaggcca
      481 tcggcgacgg caaacgggcg cgcaaggcga gtgggatgct gatgctggag gacggcacgc
      541 tgtacatgtt cgtgcggaat gtcgccaaca gccagctcgg ctggtcgagc gaccagggcc
      601 gcacgtggga gtgggccgat tggaagttcg aaacgagctt cggctgcccg acgttcctca
      661 acttcggccg ggccaacgcc ggcaatcgcg atgagtacgc ctatgtctat tcgcacgact
      721 ccgacagcgc ctacgaacga gccgatcgca tggcgctggc ccgcgtgccg caggacctcc
      781 ttcgcgagaa ggcagcatac gagttcttcg tgaagcgtga tgacgccggc gccgcggtat
      841 ggaccagcga gatcgccagg cgcggaacgg tcttcgagaa tcccagagcg tgttaccgct
      901 ccagcgtcgt ctataacgcg ccgctgaagc ggtacctgtg gtgccagact ggcgctggcg
      961 acgatactcg cttcgccggc ggcttcgcca tttacgacgc gcccgagccg tggggaccgt
     1021 ggaccgtggc ctatcgcacg gagcagtggg atgttggccc cggcgaatcg agcggcattc
     1081 cagccaagtg gatcagcccc gacggcttga cgttctggtt ggtattctcc ggcgacgatt
     1141 gtttctcggt ccgcaaggga atgctacggc ggaagccatg acgaggcctg ccatttcgac
     1201 gaccgccgag cgcgaagccg ctttgctggc gcgctatgcg atgcacagcg ccgattcgcg
     1261 cggccgcaag caccaggaag ccgagcactc ctatcgcggc ccctttcagc gcgatcgcga
     1321 tcggatcgtc cattcgtccg ccttccgccg cctgagcggc aagatgcagg tcttcacggg
     1381 ggagcggggc gactaccacc gcacgcggct gacgcacacg cacgaggtcg cgtcgatcgc
     1441 ccgtacgatt ggccgggcgc tgcggctcaa tgaggacctt gtcgaggcgc tggccctgtt
     1501 tcacgacatc ggccacccgc cattcggcca cgccggcgag gatgcactcg acgaatgcct
     1561 tcgagaacac ggcgggtttt cgcacaatcg ctacgccctg acgatcgccg agcagctcga
     1621 agcccgctat ccggcatttc ccggcctcaa cctcacgtac gaagtcctgg aaggtcaggc
     1681 cacgcgagcc gacaaagcgg cgacgtacgg cagcgatgtt gcgccgctct tggaggtgca
     1741 ggtcgtcgaa gcagccgaca gcatgaccta cgacgcccac gacaccgacg atgccgtcaa
     1801 gctgcggctg gtgacgatcg aggaactggc cgccaactcg ctgatcgccg aggctctgtc
     1861 gttggtccgc ggaggttaca ccaacctgcg gcccgacctg cttcgcaagg cggtggttca
     1921 ccagctcatc gatcggcagg tcaccgatct gctggaagcg acatcgccgc tcttggcgca
     1981 atctgcatcg cttaacgccg acgaggtccg ccgcacgagc ctgcggattg ctccgtcgcg
     2041 cgagctggcc gagcagaagc gcgagctgga acgctttctc tacgaccgcg tctatcgtca
     2101 cccggagctg atcgccgttc gctcgcacgc acaaggcagg ctccgcgaga tgtttgaggg
     2161 attcgtccaa aagcccgatc tgttgccgcc gtggtttcaa gaccgggcga atgccgtcgg
     2221 cttgccgcga gccgtgggcg actacctcgc cggcatgacc gaccgcttct gcgatcagca
     2281 gtacgaactg catttcgccc cctgactttg cccgtcaaga aatgcagcag cccggctgga
     2341 tgaccggccg agccgcattc cacaagaacg ggtctcccgc cgggtacccc agattcgccg
     2401 tgcattaccg cgatcgccag aacaatcgct gatcgatttg caggcgctct tgaaccgcgc
     2461 gaatgtcgcg gagaaaccgc caggaatccg tgtggtatcc cgcggtggga gccaggcccg
     2521 ggacgtgccg ctgccagaag gcattgaagg ggcgcattgc cagctcgcgg aggtacttgg
     2581 cggtcattga ggccagcgcc gtcgggagca tgcgctcgcc gctgacgagg aagcggaatt
     2641 cgacggttcg ccctgcgtgc tgcacttgat agacgcccaa ttccggcgtt tcccggcgaa
     2701 tcttaaccaa ttcgtcggga aagacgtgct gcaagaggcc cgcgtagtaa tcgcggccgc
     2761 catgcttgtc gcaggtgacg attgcggggc agttgggcag gcgttcgagc gtgccgcgga
     2821 cgagggccag cgttgccagc gacaggactt cggccttgtt gccgtgtcgt tcaaccagcc
     2881 cgttgaactc cgcgggaaaa atcgcccgcg cccgcaattc gcggcagcgg acgtccgctc
     2941 gcgctagccc ctcgcccagc ttcacgatgt cgggctctgc ttccggtccg caatcgacgg
     3001 gcaattgttc atcgtagccg tcgtgccagg gcgcggcgtc ttgcaggttt gcatcggccg
     3061 gatcgacccg tgaccagatt tcccgccagc gagccgggcg gcggtcgagg aggcccagaa
     3121 tggctcgcac gccgcgttcc aggagcctca gcggcgcgga cctcgccgcg gtgctggcgc
     3181 cgcgctgagc gggtttgtac aggagtttgg aatcgccaat ggccagtcgg tcgtcagact
     3241 tccgtgcctt ttcgccccct ggctgtgaaa tgatcttcga aacgcgggcg aagagttttt
     3301 ccggcccgac tcgctccgga acctgccagg cggagaggcc gatcaccagc ggacccatgt
     3361 tcggaccata tcccgcttca tcgacaccca gcaaataggt catggcggcc gtgcccgtgc
     3421 gagggaccct tccagctttg ccagtgtaac aactgttggt gctggcgggc gggagggtag
     3481 aatcagaatc cagcacctgg tgaccgggag gtatttcgat gcaagtttcg gatccaagcc
     3541 cttggcggca ggcagccagg ctgttggccg gcgccgcgct gggcggaatg tgcgtgtggg
     3601 ctttcgccca ggagggcggc gagagcgaag agtcccagcc gcgagtgccc tttgagccgc
     3661 gggcggtgga gatcaccttc accgacggga gcaatctgcg cctgcacctg gcggacgagg
     3721 tgatcgagat gtccagcccg cacggcaagc tgctgatccc cgctgaggac gtgctgcgga
     3781 tcgaatttgc ccagcgcctg ccggctgaaa cgatgcggca gatcggcgag ctgctgacgc
     3841 agctcaagga cacggacgaa gacatgcggg ccaacgccgg cgaggggttg ctggccttcg
     3901 gcccgaaggc gtatttgccg gtccttaagg cttccaagag cggcgacccg gcgctggcgc
     3961 cgattgccgg gcaggtgctg caggagctgc gggcgcaagt tcccgcggac gaactgcagg
     4021 ttcgcgaaga cgacttgatt gaaacacccg atacgaaaat cgccggccgg atcacgatgc
     4081 catcccttaa gctgaagacg agccagtttg gggagctgtc gatgaatctg gccgatgccc
     4141 gcagcctccg gcatcggtcg ctgctggcca cagcgccgga gccggacgag gaacgcggcg
     4201 tgctgcccga tccaggcaac ctcaaggcgt acgagcagaa gattggcgaa gtcttcgagt
     4261 tcagcgtcac tggtgcggtg ggcggcgcca cgatctgggg caccgatacg tacacgaccg
     4321 attccagcct ggcgatggct gccgtccacg ccggcgtgct caaggacggc gagacgggcg
     4381 tggtgaaggt gaaaatcgtc gccggccagc cgagctatgc cggctctgcg aagaacgggg
     4441 tgacgagctc gggctatggc ggctacggcg ggtcgtacga gttcgtcaaa gccgccgccg
     4501 ccggccgccc cggcgggcgc ttccgcggcc cgcggttctt gccgaaatag acatccgcaa
     4561 cgaactcctg cttcattgcg tcctttgcga tcttctgttc ccctgccgga gagcggtcag
     4621 tttgaacaga aggacgcaga ggtcgcagag aagttgatcc acaacttact ttcacgagga
     4681 attgttgaaa tgccgtttgt ccgctcgttc gtcgtggctc tcgttgtcgc gtgcactggc
     4741 aggctcatcg ccgccgaggc cgagtggccg cagtttcggg ggccgggcgg gcaggggcat
     4801 gcggtggccg agcgtctgcc gacgacgtgg agcgagacgg agaacgtcgc ctggaaatgc
     4861 ccgcttccgg gcaagggctg gtcgagcccg gtgatcgcgg gcaaccagat ttggatgacg
     4921 acggcggtcg aatcgccgat ttccgacgaa gagaaggcca ggcgcctcaa ggaaacgacg
     4981 aacacgcagc cgctggtcgt ctcgggcagc ctcagcctgc gggctttgtg cgtcgatcgc
     5041 acgagcggca agctgctgca cgacgtggaa ctgctggtcg agccccgccc gcagccgacg
     5101 cacgcgatga acagcttcgc cagccccacg ccggtgatcg aggacggcaa gctgtattgc
     5161 catttcggcg catacggcac ggcctgcctc gatacggcca cgcagaaagt ggtgtggagc
     5221 aaccgcgaga cggtgatcaa tcacgaaaac ggtccgggca gctcgccgat cctggcgggc
     5281 gacgtgctca ttttccactg cgacggcagc gaccagcagt acgtcgccgc gctcgacaag
     5341 caaaccggca agctcgcctg gcggaccgat cgcagcggtg aaatgaacgc cgagccacag
     5401 ctcaaaaagt cctacggcac gccgctggtc gtcgaaatcg gcggccagcc gcaagtggtt
     5461 tcgcccggcg cggattggct ctacgcgtat gagccccagt cgggccgcga gctgtggaaa
     5521 gtgccctacg aaaaactcgg gttctcaatc gccccgcggc cggtcgccgg gcatgggatg
     5581 atcttcatgt ccacggcttt catgcagccg gagattctgg ccgtcaagta cgacgg
//
LOCUS       DASUGB010000189         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1688861, whole genome shotgun sequence.
ACCESSION   DASUGB010000189 DASUGB010000000
VERSION     DASUGB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688861"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..462)
                     /locus_tag="VFV87_03720"
     CDS             complement(118..462)
                     /locus_tag="VFV87_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102891.1"
                     /translation="MKIALADWFWLVAYVALVAAVAGLMFRARGSMLAIYGTDAAQAE
                     WDAWREDAREMASGSGPVKRREPKSAEPPALVLMRDYFAACLGLAVLLSSVLFGTFMV
                     FLRGALRQRGPR"
     gene            complement(459..1775)
                     /locus_tag="VFV87_03725"
     CDS             complement(459..1775)
                     /locus_tag="VFV87_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5102892.1"
                     /translation="MLAHLRMWIGCSFLLVVVSAASLAVTARAEEPQAWAKAHLEELV
                     ELYRHFHAHPELSFQEKETAARLAKELRAAGAEVTENVGGTGVVGILKNGAGRAVMLR
                     TDLDALPVTEQTGLVYASQVKVKGEAGETGVMHACGHDIHITNLIAVARWFAANKDAW
                     KGTLIFLGQPAEERGSGARAMLEDGLFEKFPKPDYAVALHCDSSLATGKVGVRGGYAL
                     ANVDSVDITLLGKGGHGAYPHTTIDPIVMAAQLVLSLQTIVSREIKPIEPCVITVGSI
                     HGGTKHNIIGADCHLQITVRSYSDEVRQQLHDAIVRKAKGVAVGAGAPEPKIVFSEGT
                     PAMFNDEKLAGRLREVFVRELGTANIDDPEPSMGGEDFSEYGRAGVPILMFRLGTIEP
                     ARLARMKQLGQEMPSLHSPLYYPDAEPTLVTGLQTTVAAARELLKK"
     gene            2514..2705
                     /locus_tag="VFV87_03730"
     CDS             2514..2705
                     /locus_tag="VFV87_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102893.1"
                     /translation="MFRFTIREVLGLTTIAAISFPAANLFAPADPIAQSIMALYLAVF
                     GAGCFIGGVALGRRSKISS"
     gene            2786..3001
                     /locus_tag="VFV87_03735"
     CDS             2786..3001
                     /locus_tag="VFV87_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102894.1"
                     /translation="MRYRLRTLLIFMALAPPLLAVIWQFLPALTSPAEVAVVVALAVS
                     FTFGLSLGLGIEAIVGLVNKSGTRSKP"
BASE COUNT          505 a         1061 c          879 g          568 t
ORIGIN      
        1 agagaacagg atcaatgtgg tccgacgggc ctccgtcgga caaggggcgc tagttagggt
       61 tgatcagtgg ttaggttgac acgctgcacc tgctcggcgg ggaccgccga gccactttta
      121 tcttgggccg cgttgccgca gggcgccgcg aaggaacacc atgaacgtac caaacaggac
      181 gctggagagc agcacggcca gtcccaggca ggcggcgaaa tagtcgcgca tcagcaccag
      241 ggccggcggc tcggcgcttt tgggctcgcg ccgcttgacc ggcccgctgc ccgaggccat
      301 ttcccgggca tcctcgcgcc aagcgtccca ctccgcttgg gccgcgtccg ttccgtaaat
      361 tgccagcatg ctgccgcgcg cccgaaacat cagcccggcg accgccgcca ccagggcgac
      421 ataggccacg agccaaaacc agtccgccag ggcgattttc actttttcaa gagctcccgc
      481 gccgccgcca cggtggtttg cagccccgtg accagcgtcg gctcggcatc cggataatac
      541 agcggcgagt gcagcgacgg catttcctgg cccagctgct tcatccgcgc caggcgggcc
      601 ggctcgatcg tccccagccg gaacatcagg atcggcacgc ctgcccgccc gtattcgctg
      661 aaatcctcgc ctcccatgct cggctcgggg tcgtcgatgt tggctgtgcc gagctcgcgg
      721 acgaacactt cccgcaggcg gccggccagc ttctcgtcgt tgaacatcgc gggcgtccct
      781 tccgaaaaga cgatcttcgg ctcgggcgct ccggccccca cggccactcc tttcgccttc
      841 cgcacgatcg cgtcgtgcaa ttgctgccgc acctcgtcgc tgtagctccg caccgtgatt
      901 tgcaggtggc agtcggcccc gatgatgttg tgcttggtcc cgccgtggat tgagccgacg
      961 gtgatcacgc agggctcgat cggcttgatc tcgcggctga cgatggtttg caggctcagg
     1021 accaattggg cggccatcac gatcgggtcg atcgtggtgt gcggatacgc cccgtgccct
     1081 cctttgccca gcagcgtgat atcgacgcta tcgacgttcg ccagggcata gccgccgcgc
     1141 acgccgacct tgccggtggc caggctcgaa tcgcagtgca gggccaccgc gtaatccggc
     1201 ttggggaatt tttcgaacag cccgtcctcc agcatcgccc tcgccccgct gccgcgttct
     1261 tccgccggct ggccgaggaa aatgagcgtc cctttccacg cgtccttatt tgcggcgaac
     1321 cagcgggcca cggcgatcag gttcgtgatg tgaatatcgt ggccgcaggc gtgcatcacg
     1381 cctgtctcgc ccgcttcccc cttcaccttc acctgcgagg catagaccag ccccgtctgc
     1441 tcggttaccg gcagggcgtc caggtcggtg cgcagcatca cggcccgccc cgcgccgttc
     1501 ttcaagatgc cgacgacgcc cgtcccgccg acattctccg tcacctccgc ccccgccgcc
     1561 cgcaactcct tcgccaaccg ggccgccgtc tctttctcct ggaacgacag ctctgggtgc
     1621 gcatgaaagt ggcgatacag ctcgaccagc tcctccagat gcgccttcgc ccacgcttgc
     1681 ggctcctcgg cccgcgcggt tacagcgagg cttgcggcgc taacaacgac gagcagaaac
     1741 gagcagccga tccacattcg aaggtgcgcg agcatcggaa gatcccccga tcaagatttg
     1801 gccagttcaa ctagttgcca atacggattc ctcacacggt atcacgaatc ggagtaggac
     1861 gggtttccca acccgtccgc cgcccgcttc gatgcgtcgc aaccaacacc ccccgaccac
     1921 cgccctttcg cagcgctgaa cagggtgcgc acatcgcgcc ggcgcgcaac tttcggaaga
     1981 ttttcgcggc gactcgcgac gcgcaacctc ctcacaactc ccatccccga aaccacttgc
     2041 gctgcgcaac accccccgcc tgaggatgtt gcgctgttgc gcgttttggg ctgctcgtcc
     2101 aagaaccatg aacaggccct gaacagggga gcaaccgacg cagccgcgtt gttgcgatgt
     2161 ccagccattt cgcgcgtcct ctcctgcatg gtcaggccgt gactttcgtc tcgaagccca
     2221 agcgcctttc gtcctgccat cgacccgaat ccgtgcttcg acaacccacc atcattagac
     2281 gcacgacccc ggacacgtct ggtccactta ttcaagcaaa aaagaaaatc ttttggaaag
     2341 cggggaagcc ccatttcggc gatggcagtt gtgcgacaga cgttgggcac ttcgccctcc
     2401 gcccgcctag aatttgggaa tgcgctaccg cctccgcacg ctgctgatcg tgctggcctt
     2461 ggggccgccg gtgctggcgg cgctgtggtc cgtcgatcaa cgctaggagc cgcatgttcc
     2521 gctttacgat tcgcgaggtg ctggggctca cgacgatcgc ggcgatctcc tttcccgccg
     2581 cgaatctgtt cgcaccggct gatcccattg cgcagtcgat catggcactc tacctggcgg
     2641 tgttcggcgc gggctgcttc atcggcgggg tagcgctggg ccgtcgcagc aagatctcaa
     2701 gctagaagac ctccactacg accagccccg gtcgctgctg aaaccggaat agcgagggat
     2761 cgacgcgcgg gcgtaagata aggcaatgcg ctaccgcctc cgcacgctgc tgatcttcat
     2821 ggctctggcg ccgccgctgt tggctgtcat ttggcagttc ctgccggcgc tcacctcgcc
     2881 tgccgaagtc gccgtcgtcg tcgccctcgc cgtctcgttc accttcggcc tctcgctggg
     2941 actcggcatc gaggcgatcg tcggactcgt gaataagtcg ggcactcgaa gcaagccata
     3001 gttccatgca cca
//
LOCUS       DASUGB010000190         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1828048, whole genome shotgun sequence.
ACCESSION   DASUGB010000190 DASUGB010000000
VERSION     DASUGB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828048"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFV87_03740"
     CDS             <1..450
                     /locus_tag="VFV87_03740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5102895.1"
                     /translation="DAVWPRVAAAVPAARFRIAGRRPVAPVLALAERPGIAVAADVPD
                     MAQEIGRCWVSIAPMQSGVGIKNKVLEAWACARPVVLTPLATNGLTLPPGQGALVHAG
                     GDAMADAVIRLFGDAATRLRLGQSAQAHVRTHFTWASAARRIEALLR"
     gene            complement(727..1821)
                     /locus_tag="VFV87_03745"
     CDS             complement(727..1821)
                     /locus_tag="VFV87_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102896.1"
                     /translation="MTTARFDHFEPILRRSGATAVRHPDTLVAREATYEVFYIPFEHV
                     NWRARLVIVGITPGMNQLEMAYAEAQRLLHLGMSHAEILEAVKALAAFGGEAMRPNLL
                     RMLRHFNFAKMLEISDETDLWDGASALLHSTSVVPHAAFKNGRMFSGSFDEIIKVRAL
                     RERFENDFVPALSELADDALYVALGKTPYDALKHCVGLGVIREDQLLGALAHPSRSGG
                     SQVAVYLGEKTLADLDPADPVRGRVEWLTGAYADMKSAIVGRNGANPKPASSSLAVAS
                     KAVSPTPNANPPIGKTTRIVAVAKKEASTAPETGLHYVVSRGKAAGTVLRPHEQDGHY
                     IVSPTRFEADYQRIPLSQPLEPYLRASAFE"
     gene            complement(1818..2033)
                     /locus_tag="VFV87_03750"
     CDS             complement(1818..2033)
                     /locus_tag="VFV87_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102897.1"
                     /translation="MTRVFGLTTDPARTRPELFEKHGRGVVVVAPVQEYVLLTCKSAV
                     GFQQGQARQSVSPQAPIIDIGHRIPSQ"
BASE COUNT          391 a          681 c          721 g          432 t
ORIGIN      
        1 gacgcggtgt ggccgcgcgt ggcggcggcg gtgccggccg cgcgcttccg cattgccggt
       61 cgccgtccgg tcgcgccggt gctggcgctg gccgaacggc cgggcattgc ggtggccgcc
      121 gatgtgccgg acatggcgca ggagatcggc cgttgctggg tgtcgatcgc gccgatgcaa
      181 tcgggcgtcg gcatcaagaa caaggtgctg gaggcctggg cctgcgcccg ccccgtagtg
      241 ctgacgccgc tggcgaccaa cggtctgacg ctgccgcccg gccagggcgc tttggtgcac
      301 gccggtggcg atgcgatggc cgatgcggtg atccggctgt tcggcgatgc cgccacccgg
      361 ctgcgtctgg ggcagtcggc gcaggcccat gtgcggacgc atttcacatg ggccagcgcc
      421 gcccgccgga tcgaagcact gctgcgctga ggatctgcgg ccggcaggct gcgcttgggg
      481 cacccgatgg aagcggcggc gtccagcggt tcgatggtct ggcgggccac gaccgacgat
      541 gatgagcact atgtctacgc catagcccta tgattatccg cttcccttcc cgcaagtgct
      601 ctaccgcgac gcgcgaccaa cggatcgcga tcgctcgcga ccattcgcca ttgggttacc
      661 cagccgattt atcccgaccg aggatcgagc cgggcgcgat caagctcggg gcgacgcctg
      721 gtgcgctcat tcgaaggctg aggccctgag gtaaggttca agaggctggc tgagcggaat
      781 gcgctggtag tcggcctcaa accgtgtcgg gctcacgata tagtgcccgt cctgttcgtg
      841 tggccgcagc acagtgcctg ccgcctttcc acgactgacg acataatgga gccccgtttc
      901 tggggcggtc gaggcttcct tctttgcaac cgccacgatt ctggttgtct ttcctatggg
      961 agggttagca ttgggcgtgg ggctgaccgc cttcgaagcc acagctaatg acgacgaggc
     1021 aggcttgggg ttagcgccat tacgcccgac tatggcagat ttcatatcgg catacgcgcc
     1081 ggtgagccat tcgacgcgcc ctcgcactgg atctgcggga tcaaggtcgg ccaaagtctt
     1141 ctcacccaga tagactgcga cctgactgcc tccgctccgg gacggatgcg ccagtgcccc
     1201 gagcaactga tcttccctga tcactcccag cccaacgcag tgcttcagcg cgtcatacgg
     1261 cgtcttcccg agcgctacat agagcgcatc gtcggccagt tcggaaaggg cgggaacaaa
     1321 gtcgttctcg aaccgctcgc gcagcgcacg aaccttgatg atttcgtcga agctcccgct
     1381 gaacatcctg ccgttcttga aggcggcgtg aggcactaca gaggtggagt ggagcagcgc
     1441 cgaggcgcca tcccagaggt ccgtttcgtc gcttatctcc aacatcttgg cgaagttgaa
     1501 atgccgaagc atgcgcaaaa ggttcggcct catcgcctcg ccaccgaacg cggcgagcgc
     1561 tttcaccgct tctagaattt ccgcgtggct cattcccaaa tgtagcaggc gctgcgcctc
     1621 ggcgtaggcc atttcaagtt ggttcattcc aggggtaatg ccaacgatca cgagacgggc
     1681 acgccaattt acgtgctcga acggaatata gaaaacctcg tatgtcgctt ctcttgcgac
     1741 gagtgtgtcc ggatgacgaa cagcggtcgc accacttctc cggagaattg gttcgaagtg
     1801 gtcaaagcga gcagttgtca ctgtgaggga atcctgtggc caatgtcgat gatgggggct
     1861 tggggcgaga cggactgtcg tgcctgtccc tgttgaaatc cgaccgccga tttgcaggtc
     1921 aaaagcacat actcttgcac gggggcgacc accacaacgc cgcgcccgtg cttctcgaac
     1981 agctcgggcc gtgtccgggc ggggtcggtc gtcagcccga aaacgcgcgt catgttgcac
     2041 gccagtacga ctttgaaatc cgaacccagt agcgatccgc tgcgcaaaag acggccgggc
     2101 gaccatttcg ccaacctgtt tcggttgcgg ccgggagggg agtagcgggc ggatttcgga
     2161 gggacggttc tgcgacggca agataaaagc gcctctccct ttggtcgatt tgctgtggtc
     2221 gatca
//
LOCUS       DASUGB010000191         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1271668, whole genome shotgun sequence.
ACCESSION   DASUGB010000191 DASUGB010000000
VERSION     DASUGB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271668"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFV87_03755"
     CDS             complement(<1..551)
                     /locus_tag="VFV87_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5102898.1"
                     /translation="MALRSPIHGVPTGWQAGLLALVGGPVQRRLAQWASVLEQINAFE
                     PDLEKEGNAELRKRSLSLRYRAKAGEPLHRLVPEGYALVREAARRTIGQRHYDVQMIG
                     GLALFHGCIAEMETGEGKTLTATLPLYLHALVGKGVHLATVNDYLAERDATFLGPIFG
                     LLGLTTGVVLTKDQSKDRRKAYS"
     gene            complement(589..810)
                     /locus_tag="VFV87_03760"
     CDS             complement(589..810)
                     /locus_tag="VFV87_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102899.1"
                     /translation="MPELPRDSDRFPTRARMCQHARPANLANRLCWFSVTMTLPIEQN
                     QPIAPVATPQALLAAIDAPLAQTSSTMEA"
     gene            963..2243
                     /locus_tag="VFV87_03765"
     CDS             963..2243
                     /locus_tag="VFV87_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HEX5102900.1"
                     /translation="MKLQTMLLVIAAAWLAAVPSRGLSQDFGPYAPQPAGWYDASLQE
                     GANPALLNPAEDLCCDDGCCGSCSRCCKNHSLWGSAEFLMWWGKGSHLPPLVTTNPQG
                     TLQADAGRLDNPTTTVLFGNELVGEELQYGARLTFGLWLDPEHNVSAGGRVYGLGGDQ
                     EGFFANSAGDPILARPFFNALLGQQDALLVAFPGVVQGSITANYETDNFIGSEAFMTI
                     MMERDCRRRVDLLLGYHFLRMDDSLTINSTSTVTEVGGNLPQGTTFNLTDIFRTQNEF
                     HGGEIGFKGRMANGCWSLDGLAKVSLGAQRQQVAINGFGDVTIPPGGPVPLNGGFLAL
                     PTNIGTFERTRFVIIPEATLNLTYHFSPCLSAHVGYNIIWLSEAVTSGDQIDLALNLS
                     QQAGPLVGPARPAVLFHERDYWVQGINLGVNWDF"
     gene            2385..>3059
                     /locus_tag="VFV87_03770"
     CDS             2385..>3059
                     /locus_tag="VFV87_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5102901.1"
                     /translation="MPPFIPVSRRTFLHTSAALAAAAAAWPRRLMAAGEGLPEGKDLV
                     VRSAMPFNAEPALAELVAEAITPVSLFYVRNHGPTPKLAEEGYKLRVEGLVHNPREWS
                     LADIRCTAAQAEQEATLTCAGNRRLEMSAIKPVAGVQWDAGAIGHARWMGARLASVLE
                     AAELKPDAKHVWFEGLDPIKEKDGSVAPFGGSIPLARALAMDNPVLLAHEMNGQPLTA
                     EHGAPLR"
BASE COUNT          534 a          962 c         1008 g          555 t
ORIGIN      
        1 cagctatacg ctttgcggcg gtccttgctc tggtccttgg tgaggacgac gcccgtcgtc
       61 aggccgagca agccgaaaat cggcccgaga aacgtggcgt cgcgctcggc aaggtagtcg
      121 ttgacggttg ccaagtgaac gcccttgccc acgagggcgt gcagataaag cgggagcgtg
      181 gcggtgagcg tcttgccttc gcccgtctcc atctcggcga tgcagccgtg aaagagggcc
      241 aggccgccga tcatctgcac gtcgtagtgg cgctggccga tcgtgcgccg ggccgcttcg
      301 cgcacgaggg catagccttc gggaaccagg cgatggagag gttcacccgc tttggcgcga
      361 tagcggagcg aaaggctgcg cttgcgaagt tcggcgttgc cttctttttc caggtccggt
      421 tcaaaggcgt taatctgctc caggacgctg gcccactgag ccaaccgccg ctggacgggt
      481 ccgccgacca gcgccagcaa tcccgcctgc cagccggtgg ggacgccgtg aatgggtgag
      541 cggagagcca tggccaggag ccgaaggttc aggggtcgga aatcagcttt acgcttccat
      601 cgtagatgaa gtttgcgcca gcggcgcgtc aatggcagct agcagcgcct gcggtgttgc
      661 aaccggtgca attggttggt tttgctcaat cggcaaagtc atcgtaaccg aaaaccagca
      721 gagacgattc gccagattgg ccgggcgcgc atgctggcac attctggcgc gtgtcgggaa
      781 acgatcgctg tcgcgcggca attccggcaa agtctgccgt caggggcgac attgctggat
      841 tttccggttc tggcggtcga tccattggta gcgatgggcg aaggtgtgct gtgttgtacc
      901 tccgcactgt gattcgactc cacactgtga ttcgactccg cactgtgact agggcgcggt
      961 ccatgaaact ccagacgatg ctcctggtga tcgcggcagc ctggctggcc gctgtgccat
     1021 cgcgcgggct gtcgcaagac ttcggacctt acgcgcccca gccggctggc tggtacgacg
     1081 cttcgctgca ggaaggggcc aatccggcgt tgctgaatcc cgcggaggat ctgtgctgcg
     1141 acgacggctg ctgcgggagt tgcagccggt gctgcaaaaa tcactcgctc tggggcagcg
     1201 cggaattcct gatgtggtgg ggcaaagggt cgcacttgcc gccgttggtc acgaccaatc
     1261 cgcaaggcac gttgcaggcg gacgccggcc ggctcgacaa tccaaccacc acggtgctgt
     1321 tcggcaatga actcgttggc gaagagctcc aatacggcgc gcgcctgacg tttggcctgt
     1381 ggctcgatcc ggaacacaac gtctctgccg gcggccgggt ctatggcctc gggggcgacc
     1441 aggaagggtt cttcgccaat tccgcgggcg atccaattct ggcacggccc ttcttcaacg
     1501 ccctgctcgg ccagcaggat gccctgctcg tcgcctttcc gggcgtggtc caagggagca
     1561 ttacggccaa ctatgagacg gacaacttca tcggcagcga agcgttcatg acgatcatga
     1621 tggaacgcga ttgccgtcgc cgcgtcgatc tgctcctcgg ctatcacttt ttgcggatgg
     1681 acgattcact gacgatcaac agcacgagca ccgtcaccga agtcggcggc aatctgccgc
     1741 agggaacgac gttcaacctg accgacatct tcaggacgca aaacgagttc cacggcggcg
     1801 aaatcggctt caaaggccgc atggccaacg gctgctggtc gctcgacggc ctggccaagg
     1861 tcagccttgg tgcgcagcgg cagcaggtgg caatcaacgg ctttggggac gtgacgattc
     1921 cgccgggcgg acccgtgccg ctcaacggcg gcttcctggc cctgccgacg aacatcggca
     1981 ccttcgagcg cacccggttc gtcatcattc ccgaggcgac gctgaacctg acgtaccatt
     2041 tctcgccctg cctgagcgcc cacgtgggtt acaacatcat ctggctgtcg gaggccgtga
     2101 cttcgggcga ccagatcgac ctggcgctca atctctcgca gcaagccggc ccgctcgttg
     2161 gcccggcccg cccggccgtc ctgttccacg agcgggatta ctgggtgcag gggatcaacc
     2221 tgggcgtgaa ttgggatttc taggcgactt ggcctcggca actcttggcc gccgccgctt
     2281 cggcacctgc cgtgcaggcg tcgcaaaccg ctcacgctgc tgccaagtac tccgtactta
     2341 gtactgagta cttccgcgcg gcaatccctt gatacaattg gagcatgcca ccattcattc
     2401 cagtctcccg acgcacattc ctccatacct cggcagcgct ggccgcagcc gcggcagcct
     2461 ggccgcggcg gctgatggct gctggggaag ggctgccgga gggaaaggac ctggtcgtcc
     2521 gctcggcgat gccgttcaac gccgagccgg cgctggccga gctggttgcc gaagccatca
     2581 cgccggtcag cttgttctat gttcgcaatc acgggccgac gcccaagctc gcggaagagg
     2641 gttacaagct gcgggttgag ggcctcgtac acaacccgcg cgagtggagc ctggcggaca
     2701 tccgctgcac ggccgcgcag gcggagcagg aagcgacgct tacctgcgcc ggcaaccggc
     2761 ggctggaaat gagcgcgatc aagccggtcg cgggcgtgca gtgggatgcc ggcgcgatcg
     2821 gccatgctcg ctggatgggc gcccgactgg cgagcgtgct ggaagccgcg gagctcaagc
     2881 ccgacgccaa gcacgtctgg ttcgaggggc tcgatccgat caaggagaag gacggcagcg
     2941 tggccccttt cggcggctcg attccgctcg cgcgggcgct agcgatggac aacccggtgc
     3001 tgctcgccca cgaaatgaac ggccagccgc tgactgccga gcacggcgcg cccttgcgg
//
LOCUS       DASUGB010000192         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_21409,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000192 DASUGB010000000
VERSION     DASUGB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21409"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFV87_03775"
     CDS             complement(<1..416)
                     /locus_tag="VFV87_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5102902.1"
                     /translation="MIRVVPSIVVAIVTAVCWSVAPAEDLPHRPNVVLIMTDDQGYGD
                     LGFHGNPLIHTPQIDTLAKESVRLKYFYVSPVCTPTRACLMTGRYNYRTRAIDTFRGR
                     AMMDPAEITVAELLKEAGYATGIFGKWHLGDCYPMRA"
     gene            596..1618
                     /locus_tag="VFV87_03780"
     CDS             596..1618
                     /locus_tag="VFV87_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5102903.1"
                     /translation="MLVRVILYVASSVLVSALCLVGCGNPELPSSSVPGAGAAASGSD
                     AATSKGTVGVSVLTFTNPFFRVIGNHIEDELRQAGYDTIVVAGEKDIAAQERQVNDFI
                     SKGCVAIVLCPCDSKAIGAVIKKANAAGVPVFTADIASLDPEAKVVSHIATDNSGGGK
                     QAGEAMIEALGEGGGKVVILDYEAAESCILRVKGFREVIDAYNEGREAGKIEIVAELP
                     GDGDTQRGDKAAQDALQAHPDLAGIFAINDPSAIGAWKALERAGKTDQVKIIGFDGQD
                     FGKEAIRDGKIYADPIQHPDQIGRLTAQAILNYIDGEEVKDVQLIPTTLYRLADSLND
                     PELKKQ"
     gene            1615..2940
                     /locus_tag="VFV87_03785"
     CDS             1615..2940
                     /locus_tag="VFV87_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102904.1"
                     /translation="MNHWLLVLVREHTTVLVLALLCAVLSVVTLDEQFPAGASAAKQV
                     AARIESQVPPPAKILIVVRQTQDDAAYAAAVKEQLTKAGYDVVDTVRGQPSDARRALD
                     GLSEAGQQIQAIAANDVTAGWGPLRDLSSQGAAREAVVVVPRSYYWPNFLKATNLLNI
                     ANQIAFIAIIAIGMTLVIISGGIDLSVGSLVALTAMVAARLVRDYAGGTTAGPAGLAL
                     CCIAAIGVGGCAGAFNGALVAAMRIPPFIVTLATMSIAAGLAFMISHGESINEVPASI
                     GWLAGGTLVGEIPNGVLWMIILYGSAHVVMSRTTFGRHLYALGGNRRAAWLCGLPVRR
                     VELSAYALSGMLAGLAGVLMLSQYASASPNYGVTYELQVIAAVVVGGTSLSGGRGTIF
                     GTLLGALLIAVVQNGMNLMGLSSDPQKVVLGLVILAAAVVDRLKQGRSE"
BASE COUNT          550 a         1008 c         1029 g          545 t
ORIGIN      
        1 gcccgcatcg ggtagcaatc gcccaggtgc catttgccga agatgcccgt cgcatagccg
       61 gcttctttga gcagttccgc cacggtgatt tccgccgggt ccatcatcgc ccggccgcgg
      121 aacgtatcga tcgcccgcgt gcggtagttg taccgcccgg tcatcaggca ggcccgcgtt
      181 ggcgtgcaga ctgggctgac gtaaaagtac ttgagccgca cgctctcctt ggccagggtg
      241 tcgatctgcg gcgtgtgaat gagcgggttg ccgtgaaacc ccagatcgcc gtagccctgg
      301 tcgtccgtca tgattaggac gacattcggc cggtgcggca gatcttccgc aggagctacg
      361 gaccagcata ccgcggtcac gatcgctacg actatggaag gaacgactcg gatcatcgca
      421 gcgcctccct tcctatccaa aactcgctca gcttccctaa cataccagcg gccaccgcta
      481 ggggcgaccg gattcgtgac agcgacttcg caattggcga agtcgcccgc cgttgtgcta
      541 cgataggctc ggccggcttt tggtaggcgc acctctcccg tgctggagcg actcaatgct
      601 cgtccgcgtc attctttatg tcgcttcgtc cgtattggtg tcggcgcttt gcctggtggg
      661 gtgcggcaac cctgaattgc ccagttcgag cgtgcccggc gccggcgcgg cagccagcgg
      721 atcggatgca gcaacgtcca agggcaccgt cggcgtctcg gtactgacct ttacgaaccc
      781 gttcttccgc gtcatcggca accacatcga agacgaactg aggcaggcgg gttacgacac
      841 gatcgtcgtg gcgggcgaga aggacatcgc cgcccaggag cggcaggtga acgacttcat
      901 ctcgaaaggc tgcgtggcga ttgtcctatg cccgtgcgat agcaaggcaa tcggcgcggt
      961 catcaagaag gcgaatgccg cgggcgtccc tgtcttcacc gccgatattg ccagtctcga
     1021 tcctgaagcg aaggtcgtgt cgcacatcgc caccgacaac tccggcggcg gcaagcaggc
     1081 cggcgaggcg atgatcgaag ccctcggcga aggcggcggc aaggtcgtaa tcctcgacta
     1141 cgaagcagcc gagtcgtgca tcctccgcgt caagggcttc cgcgaggtga tcgacgccta
     1201 caacgaaggt cgcgaagccg gcaagatcga gatcgtcgct gaattacccg gcgacggcga
     1261 cacccagcgc ggcgacaagg cggcccaaga cgccctgcag gcccatcccg acctggctgg
     1321 gattttcgcg atcaacgatc cctcggccat cggcgcgtgg aaggccctgg agcgggccgg
     1381 caagaccgac caggtaaaga tcatcggctt cgacgggcag gatttcggca aggaagcgat
     1441 ccgcgatggc aagatctacg ccgatccaat ccagcacccc gatcagatcg gccggctgac
     1501 agcccaggcg atcctcaact acatcgacgg cgaagaggtc aaggacgtac aactcattcc
     1561 cacgaccctc tatcgcctgg cagacagcct gaacgacccc gagttgaaga agcagtgaac
     1621 cactggctgt tggtgctggt tcgcgagcac accaccgtcc tggtgctggc cctgctgtgc
     1681 gccgtgctga gcgtcgtcac actcgacgag cagtttcccg cgggtgcaag tgccgccaag
     1741 caggtggcgg cgcggatcga aagtcaggtt ccgccgccgg cgaagatcct gatcgtcgtg
     1801 cgccagacgc aggacgacgc agcgtatgcc gcggctgtca aggagcagct caccaaagca
     1861 ggttacgacg tcgtagatac ggtccgcgga cagccaagcg atgcccgccg cgcgctagac
     1921 gggctaagcg aagcgggcca gcagatccag gcaatcgcag ccaacgacgt aactgccggc
     1981 tggggcccgc tgcgggatct gtcgtcgcaa ggggcggctc gagaggcggt cgtcgtcgtg
     2041 ccgcggagct attactggcc gaactttctc aaggccacca atctgctcaa tatcgccaac
     2101 cagatcgcct tcatcgcgat catcgcgatc ggcatgacgc tggtgatcat ctcgggcggc
     2161 atcgacctct cggtcggcag cctggtcgcc ctcacggcga tggtcgcggc ccgcctcgtg
     2221 cgcgattacg ccggcggaac cacggcgggg ccggcggggc tcgccctgtg ctgcatcgcg
     2281 gcgatcggcg tcggcggctg cgcgggagca ttcaacgggg cgctggtcgc cgcgatgcgg
     2341 attccgccgt tcatcgtcac gctggcgacg atgtcgattg ccgcgggtct agccttcatg
     2401 atctcgcacg gcgaatcgat caacgaggtg ccggcgtcga tcggctggct ggccggcggg
     2461 acgctggtcg gcgagattcc caacggcgtg ctgtggatga tcattttgta tggatcggcg
     2521 cacgtcgtca tgtcgcggac gacgttcggg cggcacctgt acgccctggg cggcaaccgc
     2581 cgcgcggcct ggctgtgtgg cctgccggtg cggcgcgtcg agttgtccgc ctatgcgctg
     2641 agcggcatgc tggccggcct ggcgggcgtg ctgatgctct cgcaatacgc cagcgccagc
     2701 ccgaactacg gcgtcacgta cgaactgcag gtgatcgctg ccgtcgtcgt cggcggcaca
     2761 tcgctttccg ggggccgggg cacgatcttc ggcacgttgc tcggcgcgct cttgatcgcc
     2821 gtcgtccaga acggcatgaa tctcatgggc ctctccagcg acccgcaaaa agtcgtcctc
     2881 ggcctcgtca tcctcgccgc cgccgtggtc gatcggctga agcaggggcg aagcgagtag
     2941 gcgcccccga ggggtccgct gcaaagcaat taactcacgt ccaggacggc caggccgcaa
     3001 tcggcggcgg cgcttgccaa taccctcttg tcggctgcga ctagcgccgc gaggcgcgtg
     3061 cgactgtgga gtgcctgggc cgtcgccagg tgaatggcat ccagcgtcgg gatgggggtg
     3121 gttaagccgt gc
//
LOCUS       DASUGB010000193         7695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_577759, whole genome shotgun sequence.
ACCESSION   DASUGB010000193 DASUGB010000000
VERSION     DASUGB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577759"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFV87_03790"
     CDS             complement(<1..323)
                     /locus_tag="VFV87_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HEX5102905.1"
                     /translation="MRWFWIERFVEFERGRRAVAIKNVSLVEEELDEYTPGYPVMPNT
                     LIIEGMAQTAGLLIGEMTGFEARVILAKIGKAVFHRYAIPGDSLRYTAVISDVNADGA
                     TATVT"
     gene            complement(339..731)
                     /locus_tag="VFV87_03795"
     CDS             complement(339..731)
                     /locus_tag="VFV87_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5102906.1"
                     /translation="MSVTNEEVFKKVQTALVDALGVDEEEVTPQATMVGDLGAESIDF
                     LDIVFKLEKAFGIEIPRKELAPEDILTNVEYVQDGKVTPAGLAELKRRMPFVDFSKFE
                     ANPQVREFSNLLTVGDLCRYVESKLAKA"
     gene            complement(812..1300)
                     /locus_tag="VFV87_03800"
     CDS             complement(812..1300)
                     /locus_tag="VFV87_03800"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX5102907.1"
                     /translation="MRFCLLDRITSLEPGVKITAAKRLQPGEDYLRDHFPRFPVMPGV
                     LMLEAMFQASAWLIRQTEGFAHSLVLLKEARNIKYADFVTPGRELVVSAEVLKHDASL
                     TTLKVQGTIDGNVSVHGRIVVERFNLADRYPVLKDTDPYMRAELRPVLARLLSAATPS
                     PS"
     gene            1625..2173
                     /locus_tag="VFV87_03805"
     CDS             1625..2173
                     /locus_tag="VFV87_03805"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5102908.1"
                     /translation="MWLVSNGFLAGSLPFRLNPGEYIVGRAKGVAISIRDLTLSRRHA
                     RLIRDAKSLIVEDLGSRNGTFINGERVSRQTAEIGDEICFGGAVCRVAASPLAVNIED
                     GETTHTMQLASGATVEFDELTRAQMEVLRLVVEGLDESAIAARLNRSVHTIHTHLKAI
                     FRQFQVHSRAELIVKAGKGPMA"
     gene            2549..>7695
                     /locus_tag="VFV87_03810"
     CDS             2549..>7695
                     /locus_tag="VFV87_03810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5102909.1"
                     /translation="MLSASPASPELSLDAQDYDPTSILVRFKPGAAATNAADILPGAS
                     NGRAYSLVPGLRSVRLGPGQSVEAALHVFRSNPNVIYAEPNGRVQVSLVPNDDLYPSL
                     YGMEKIDAPEAWDIATGDTSVLVAVIDTGIDYTHPDLAANIWTNPGEIAGNGVDDDGN
                     GYVDDVHGYDFVGWEYGYGDGDPLDDFFHGTHVAGTIGAVGNNAEGVVGVNWNAKIAA
                     VKFLDSGGGGSWEGAIAAIQYAVAIGANISNNSWGGTEFSAALHDAIAAARDAGHLFV
                     AAAGNNYSNGDSSPSYPAGYSVDRIVDGITYEGLDNIIAVAATDSGDNKADFSNWGLS
                     SVHLGAPGVGIYSTFPTYETEAMYWNGFATNYETISGTSMATPHVAGVAALAWGLAGS
                     SNYAEIKSAILSSVDPVSSLATTGPTPVATGGRLNAFKTLQAVGMSIAGSTPAAGSII
                     AATSTDFAVNFSHPYDPATIDDPDLTVNGIAPTSHTFTDSDTVTFHFATSPVTAQGLQ
                     TMHMAAGAVSAASAAGLPDPLLHEWTATFRYDLERMEVVSTSPAGPLAGVPLTKLDIN
                     LNEAVDPLSVSTSDLALSLGWVTGVEVLPGNTTIRFTLAGITEEGSLDVGLAAGAFTD
                     EFGNPSLPYSADFTLVTGMGVGGSTPAAGNIILGLSTPSEYVVHFTLPYDADTVDAGD
                     LTVNGIAATSRTLTDSDTVTFHFAASPVTIQGEQTMHIAAGALNADPGAGLPDPAIQE
                     WTGLFRWDQAPMEVTSTSPASSSNVLLPFTTLDVNLNEAVQPSSVQTSDLQLSQGSAM
                     GFTLENGGATIRFTLSGITSEGTLSASIAAGALADTYGNPNLAFAGSFTLDIGTVPFP
                     TPLAPVAPLGSLIQSQVAAGIIGVAGDTDSFTIDVAAGQTITVLVTPASTLQPTVAIG
                     GTSASGSAPGKKALLQTVAAGGLQTIAVGALGGTTGGYTVQVLLDTALETESHDGAGN
                     NTTPQNIDGSFITVATTTVSADRGAVMGRTDGNPTVTLLAANFETTTGDNNWTIDTGT
                     NNLWHNSIGHRYDAGHSATHSMYFGAGEKYVKKGNKPETFTEGTYEVTKKGKGGGAQV
                     AAGTITSPNIALATGGQISVDFNYLLQTQSAAGLDVAELQIKPIGSSAWTTLATYNAV
                     AESSTWIAASTVDISTFAGQTVQLRFSFDTVTAQDNKFEGWYVDDVVVRQTTPHDNYS
                     FHVEAGERVTVALQAAGDVNVLLQDSGGATLAAGSSGAVNLRKMINNFGPLTAGTYRL
                     AITGQTNTPYTLVVLRDASFDAESNDSYATAQDYTGVPGVLGHVTAGSDLFSLRGTPV
                     SGGLVLAGSKMTLGIATDGSFITGGAGIQFLGNEFVIPGSPVAGFTIGQNGSNYTNKE
                     ALGTTDIPVSLEDLSLGTFRGVRIVGNVGGGLQLERVIAFSQDDEFATIATRLTNTSG
                     GTIDNVAWLENLDPDQGEPLGFGFETYNDVVLGGQFVRADAAEGLTIGLGSADARRVV
                     SAQGFDNRDPFEIINSPVDPDGLWEDIGINLAFNFGSLAPGQMAAGSMLMTFGRTTAE
                     AEATYTAHTGGTLVADEDWYKQTVGADGILHVETSTPGDAPGEPGNALNPRIEVYSTS
                     ATAPVYLGTPLADGRNEVVHVTGLVPGTIYRIRVSAEGGTGGAYFLDPPPAGAGDSNQ
                     AALAASPSLAGSALLTSLAAQEGDDADADDHKLPIPDDAAWSAAGVRP"
BASE COUNT         1464 a         2483 c         2375 g         1373 t
ORIGIN      
        1 ctggtgaccg tggcggtcgc tccatcggcg ttcacgtccg agatgacggc cgtatagcgc
       61 agcgaatcgc ccgggatcgc atagcggtga aagaccgcct tgccaatctt cgccaggatg
      121 acccgcgcct cgaagccggt catttcgccg atgaggaggc cggccgtttg ggccatcccc
      181 tcgataatca gcgtgttggg catcaccgga tagcccggcg tgtattcgtc cagttcttcc
      241 tcgacgagcg agacgttctt gatcgccacc gctcggcggc cgcgctcgaa ttcgacgaag
      301 cgttcgatcc agaaccagcg catcggaact gagcttcctt aggccttggc cagcttcgat
      361 tcgacgtacc ggcacagatc gcccaccgtc agcaggttgc tgaactcgcg gacctggggg
      421 ttggcctcga actttgagaa atcgacgaac ggcatccgcc gcttcagctc cgcgaggccc
      481 gccggcgtga ccttgccgtc ctgcacgtac tcgacgttcg tgaggatgtc ttccggcgcg
      541 agctctttcc gcgggatttc gatgccgaat gccttctcga gcttgaagac gatgtcgaga
      601 aagtcgattg actcggcgcc gaggtcgccg accatggtcg cttgcggcgt gacctcttcc
      661 tcatcgacgc ccaaggcgtc gaccagggcc gtttgaacct ttttgaagac ttcttcgttc
      721 gtgacagaca tcgcttggca aactccaatc ggtggcgaat tcttcagttt ggccgaacct
      781 gggccatttg ggcaacccca gcttcgcaac tttagctcgg gcttggggtt gcggcagata
      841 gcaggcgggc cagcaccggc cgcaattcgg ctcgcatgta gggatcggta tccttcagca
      901 cagggtatcg gtcggcaagg ttgaaccgct caacgacgat ccggccgtgg actgaaacat
      961 tgccgtcgat cgtcccctgg actttcagcg ttgttaggga cgcgtcgtgc ttcaagacct
     1021 ccgcgctgac gaccagttcc cttccgggcg tcacaaaatc ggcatatttg atatttcggg
     1081 cctctttgag cagcaccagg gaatgggcga agccctccgt ctgccggatg agccaggcac
     1141 tggcctggaa catcgcctcg agcatcaaaa cacccggcat cacggggaac cgcggaaagt
     1201 ggtcccggag gtaatcttcg cccggctgca ggcgcttcgc ggccgtgatc tttacccccg
     1261 gttcgaggga cgtaatccga tccagcaaac agaaccgcat aagccggtct cgaaaccagg
     1321 tacgcccgtt ccgggggcgc gaattgcatc cttccgatca acagcggtct gaatatagct
     1381 ggggtgattc ggggcggcaa cccgcaagca ggcgttaagg cgagcatcag ccagttgtga
     1441 cctgaacatc tgctggcgcg caaggaggga tccccggccg gtgacagaat ctgcttgaga
     1501 attcgttccc cttaggtcat tatgctatcc cagacggagg ctagtttcgc aggcgtcgat
     1561 tggtggcagg cccaattctc tggtcccacg ccgcgacttg tgccaacatc gtgcgagtct
     1621 ttctatgtgg ctggtcagca acgggttcct ggctggcagt cttccctttc gcctaaatcc
     1681 gggcgagtac atcgtcggcc gagcaaaagg cgtagctatc tcgataagag acctgacgct
     1741 ctcccgtcgc cacgccaggc tgatccgcga cgcgaaatcg ctgattgtcg aagatctcgg
     1801 cagccggaac ggcacgttca ttaacggcga gcgtgtcagc aggcaaacag ccgaaatcgg
     1861 cgacgagatt tgcttcggcg gcgcggtgtg ccgcgtggcc gcgtctccgt tggcggtgaa
     1921 tatcgaggac ggcgaaacga cgcacacgat gcagttggcg agcggcgcga cggtggaatt
     1981 cgatgaattg acccgcgccc agatggaagt gctgcggctg gtggtggaag ggctcgacga
     2041 atccgcaatt gccgcccgcc tgaatcgcag tgtgcacacc attcatacac atttaaaggc
     2101 aatcttccgc caatttcagg tgcattcccg ggcagaactg atcgtcaagg ctgggaaggg
     2161 accaatggcc tagtcgcgcg tcggagagaa atccagacgt cctaacctat tcagggtatg
     2221 gcgtcgttca aacgcagctt cataatcctg gacgttatgg tgacggcgtg atcgggggag
     2281 ggcgctgtca cggatcggcg ttgctcttct ttgtacggag ctgcgttacg cgagcgggcc
     2341 aatcgctgcc tctgatttcc cgcacgctgt tctctcaaaa caggcagggt tcgacccggg
     2401 agttcctcgt atgttgccca tgtcccaagg tggtggtgcg ttgggtttgc gcgcggtgaa
     2461 acagacggtc cagtcgcgac ggcatccgag acgaacgaag cgttcggcgg tgcggcagct
     2521 attcttggag cgattagagg agcgcacggt gctgagcgcg agcccggctt ccccagagtt
     2581 gtcgctcgac gcccaggatt acgatcccac gagcattctg gtgcgcttta agcccggtgc
     2641 cgcggctacg aatgctgctg acatcctgcc gggcgcgtcc aacggccgag cctactcgct
     2701 ggtgcccggc ctgcgctccg tcaggctggg gccagggcag agcgtcgaag cggccttgca
     2761 cgtttttcgc tcgaacccca acgtcatcta cgcggaacct aatggacgcg tgcaggtttc
     2821 gctcgtgccg aacgatgatc tgtatccaag cctgtacgga atggaaaaga tcgacgcccc
     2881 ggaggcctgg gacattgcca ccggcgatac cagcgtgctc gtcgcggtga tcgacaccgg
     2941 catcgactac acccatcccg acctggcggc caacatctgg accaatcccg gcgaaatcgc
     3001 cggcaacggc gtggatgacg acggcaacgg ctacgtggac gacgtccacg gctacgactt
     3061 cgtcggttgg gaatatggct atggcgacgg cgatccactc gacgattttt tccacggcac
     3121 gcacgtcgcc ggcacgatcg gtgcggtcgg gaacaatgcc gagggcgttg tcggcgtgaa
     3181 ttggaacgct aagatcgcgg ccgtgaagtt cctcgattcg ggcggcgggg gctcgtggga
     3241 aggggccatc gcggcgatcc agtatgccgt cgccatcggc gccaatatct ccaacaacag
     3301 ttggggcggg accgaattct cggccgcgct gcacgatgcc atcgcggcgg cccgcgacgc
     3361 cggccatctg ttcgtcgcgg cagccggaaa caattacagc aacggcgaca gcagtccttc
     3421 ttacccggct ggctatagcg ttgatcgtat cgtcgacggc atcacctatg aggggctgga
     3481 caacattatc gccgtcgcgg cgactgactc cggtgataac aaggcggatt tctccaactg
     3541 gggtctcagc agcgtacacc tgggcgcacc aggcgtcggc atctacagca ccttcccaac
     3601 gtatgaaacc gaagccatgt actggaacgg cttcgccacc aactatgaga cgatcagcgg
     3661 gacctccatg gccactccgc acgtggcggg agtagcagcg ctggcgtggg gattggccgg
     3721 cagctcgaat tacgccgaga tcaaaagcgc aatcctctcc agcgtcgatc cagtatcgtc
     3781 gctggccacg acgggcccga cgcccgtcgc caccggcggc cggctgaacg ccttcaagac
     3841 gctgcaagcg gtcggcatgt cgatcgccgg tagcactccg gccgcgggct cgatcatcgc
     3901 cgccacgtcg accgacttcg ccgtcaattt ctcacatccc tacgatccgg ccacgatcga
     3961 cgaccccgat ctgaccgtca acggcatcgc gccgacatcg catacgttta ccgattccga
     4021 tacggtgact ttccactttg ccacgtcgcc cgttaccgct cagggcctgc agaccatgca
     4081 catggccgcc ggcgcggttt ccgcggcttc tgctgcgggc ctgcccgacc cgctgctcca
     4141 cgaatggacg gccacgttcc gctacgatct ggagcgcatg gaagtggtct cgaccagccc
     4201 ggctggcccg ctcgcgggcg tgccgctgac gaaacttgac atcaacttaa acgaggcggt
     4261 ggacccgctc tcggtgagca cgagcgacct ggcgctcagc ctgggatggg taactggcgt
     4321 cgaagtcctg cccggcaata ccacgatccg cttcacgctg gcgggcatca cggaggaagg
     4381 ctcgctggac gtcggcctcg ccgcgggggc cttcaccgac gagttcggca atcccagcct
     4441 gccgtactcc gccgacttca cgcttgtcac cggcatgggc gtcggcggga gcacgcccgc
     4501 cgcggggaac atcattctcg gcctgtccac gccgagcgaa tacgtcgttc acttcacgct
     4561 gccgtacgac gccgacaccg tggatgccgg tgatctgacg gtcaacggaa tcgccgcgac
     4621 gtcgcggacg ctcaccgatt ccgacacggt cacgttccac ttcgccgcct cgccggtcac
     4681 catccagggc gagcaaacga tgcacatcgc cgccggcgct ttgaacgccg accccggcgc
     4741 gggccttccc gatccggcga tccaagagtg gacgggcctc ttccgctggg accaggcgcc
     4801 catggaggtc acctccacgt cgcccgcctc cagctcgaat gtgctccttc cctttacgac
     4861 cctcgacgtc aacttgaacg aggcggtcca gccatcgtcc gtgcaaacga gtgacctgca
     4921 gttgagccaa gggtccgcca tggggtttac cctggaaaat ggcggcgcca cgatccgctt
     4981 cacgctgagc ggaatcacgt ccgagggcac gctgagcgcc agcatcgctg cgggcgccct
     5041 ggccgacacc tatggcaacc cgaacctggc gttcgccggc agctttacgc tggacatagg
     5101 cacggtcccg tttccgacgc cgctggcgcc ggttgccccg ttggggagct tgatccagag
     5161 ccaggttgcc gcgggtatta tcggcgtcgc gggcgacacc gattcgttca cgatcgacgt
     5221 ggccgcgggc cagacgatca ccgtcctggt cacacccgcc agcacgctcc agccgacggt
     5281 ggccatcggc gggacatcgg cgtcggggag cgcccctggc aagaaggccc tcctgcaaac
     5341 agttgccgcc ggcggcctgc agacgattgc cgttggagct ctcggcggaa cgaccggcgg
     5401 ctacaccgtg caggtgctcc tcgacacggc cctcgaaacc gagagccacg acggggccgg
     5461 caacaacacc acgccccaga atattgacgg ctcattcatt acggtcgcga ccacgacggt
     5521 cagcgccgac cgcggcgcgg tgatgggccg gaccgatggc aatcccaccg tgacgctgct
     5581 cgccgcgaac ttcgagacga ccaccggaga caacaactgg acgatcgaca ctggcacgaa
     5641 caacctgtgg cacaattcaa tcggccaccg ctacgacgcg gggcactcgg cgacccacag
     5701 catgtacttc ggggctggag aaaagtacgt caagaaagga aacaagccgg agacgttcac
     5761 cgagggcacg tacgaagtga caaaaaaggg caaaggcggc ggcgcccaag ttgccgctgg
     5821 aacgatcacc tcgccgaaca tcgcgctggc gaccggcggg cagatctcgg tggatttcaa
     5881 ttacctgctg caaacgcaaa gcgccgccgg gctggacgtg gccgaattgc aaatcaagcc
     5941 catcggcagc agcgcctgga caacgctggc cacctacaac gccgtcgccg agagcagcac
     6001 ctggatcgcc gccagcacgg tcgatatctc gacctttgcc ggtcaaaccg tccagctccg
     6061 cttcagcttc gacaccgtga ccgcgcagga caataaattc gaaggctggt acgtggacga
     6121 tgtcgtggtt cgtcaaacca cgccgcacga caactattcc ttccacgtgg aggcaggcga
     6181 gcgcgtgacc gtggccctcc aggcagctgg cgacgtgaat gtgctgctcc aggattccgg
     6241 cggcgccaca ctggctgcgg gcagttccgg ggcggtcaat ctgcgcaaga tgatcaacaa
     6301 cttcggcccc ttgacagcgg gaacctaccg cctggccatc accggccaga cgaatacacc
     6361 ctatacgctg gtcgtgctgc gggatgcttc attcgacgcc gagagcaacg actcctatgc
     6421 cacggctcag gactacacgg gcgtgccggg agtcctcggc cacgtgacgg cgggcagcga
     6481 cttgttctcg ctccgcggca cgccggtcag cggcggcctg gtgttggcag gctccaaaat
     6541 gacgctcgga atcgccaccg acggcagctt catcaccggc ggcgcgggca ttcagttcct
     6601 cggcaacgag ttcgtcattc ccggaagccc cgtcgcgggc ttcaccatcg gccagaacgg
     6661 cagcaactac accaataagg aagctctcgg gacgacagat atcccggtca gcctcgaaga
     6721 cttgagctta ggaaccttcc gcggggtgcg cattgtgggt aatgtcggcg gcggtttgca
     6781 actcgaacgg gtcatcgcct tcagccagga tgacgaattc gccacgattg ccacccggct
     6841 taccaatacc agcgggggca cgatcgataa cgttgcctgg ctcgaaaacc tggaccccga
     6901 ccagggcgaa ccgctgggat ttggcttcga gacctacaat gacgtggtcc tcggtgggca
     6961 gttcgttcgg gccgatgccg cggagggcct gacgatcggc ctgggatcgg ccgatgcgcg
     7021 ccgcgtggtc tccgctcaag ggttcgacaa ccgcgacccg tttgaaatca tcaattcgcc
     7081 agtggacccc gatggacttt gggaagacat cggcatcaat ctcgcgttca actttggcag
     7141 cctggcgccg ggccagatgg ccgccggctc gatgctcatg accttcggcc gcaccacagc
     7201 cgaagccgag gcgacgtaca ccgcccatac cggcggcacg ctggtggccg atgaagactg
     7261 gtacaaacag accgtggggg ccgatggaat cctgcacgtg gagacgagca ctccaggcga
     7321 cgctcccggc gagcccggca atgcactcaa cccgcggatc gaggtctata gcacatcagc
     7381 gacggctccg gtctatttag gcacgcctct ggccgacggc cgcaacgagg tcgtccacgt
     7441 gactggcctg gttcccggca caatttaccg gattcgggtc tcagccgaag gcggaacggg
     7501 cggcgcgtac ttccttgatc cgccgccggc gggggccggc gattcgaacc aggccgcttt
     7561 ggctgcctcg ccatcgctcg ccggatcggc cctgctgacc agccttgcgg cgcaggaggg
     7621 agacgatgct gacgccgacg accacaagct gccgatcccc gacgatgcgg cctggtcggc
     7681 tgctggggtg cgccc
//
LOCUS       DASUGB010000194         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_577804, whole genome shotgun sequence.
ACCESSION   DASUGB010000194 DASUGB010000000
VERSION     DASUGB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577804"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VFV87_03815"
     CDS             complement(<1..1404)
                     /locus_tag="VFV87_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX5102910.1"
                     /translation="MLAVLLTSAVIGLSADLFRAVGVVFYTEQYLAGLLAIAMPLLFL
                     HVPASGGRGGRVGPVPWYDIAAALASCACSIYVLVKFPQLSELVSAPPPDGLIVATIL
                     VLLILEGLRRTTGIVLSIVTASFFVLALVGGLLPGELAAKSIPLPRLTYYVIWDSSAS
                     LGLPLKIVSTVVVIYALFGTVLFKVGGTSFFTGLSMALMGRRRGGPAKIAIIGSSLFG
                     MISGSAVSNVLTIGVVTIPLMKKVGYRPAFAAAIEACASTGGQLMPPVMGIAAFIMAE
                     FLQIPYASVALAALIPAVLFYLALFIQVDLEAARSALTPMESSQIPDIRQVLKDGWHF
                     PIPFAVLIYALFWKGYEAETAGLLGIAATLILAIAFPAGGKRIGVRGLYEALRDTGKA
                     VVELFMIGAAAGIIIGCLSYSGVGFSMTLSLVTLGGGTLIGLLVIAAFASIVLGMGMP
                     TVGVYILLATLVAPALVQ"
     gene            complement(1617..>2084)
                     /locus_tag="VFV87_03820"
     CDS             complement(1617..>2084)
                     /locus_tag="VFV87_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5102911.1"
                     /translation="AIQTAVVMLLVAASALVGIYLTESQLPQKLAASIIDITTNPYFV
                     LLILNVFFLIIGMFLHSAAAIILTVPIVLPLVHQVHIDPIHFGLVLTLNLAIGQQTPP
                     VASVLIASCSIAKSDIWATTKANLGFISVLLLVLVLCTYVPWLSLALIDMFYN"
BASE COUNT          513 a          618 c          622 g          331 t
ORIGIN      
        1 ctgcaccaag gccggagcaa ccagcgtagc caacagaatg tagacgccga ccgtcggcat
       61 tcccatcccg aggacgatgg atgcgaacgc tgcgatcacc aacaggccga tgagcgtacc
      121 gcccccaagc gtcacaaggg aaagtgtcat ggagaaacca acgccggagt aggatagaca
      181 accgataatg atgcccgcag ccgcaccgat catgaataat tcgacgaccg cctttccggt
      241 gtcgcgcagc gcttcataga ggccccggac gccgatccgc ttgccgccgg cgggaaacgc
      301 aattgccagg atcagggtag ctgcgatgcc aaggaggcca gcggtctcgg cttcgtaacc
      361 cttccagaat aacgcgtaga tcaggacggc gaacggaatg ggaaaatgcc agccatcctt
      421 gagcacctga cggatatctg gaatttgcga tgactccatt ggagtcagcg cactgcgcgc
      481 ggcctccagg tcgacttgga taaaaagcgc cagatagaac agaaccgcgg gtatcaacgc
      541 cgcgagcgcg acgctggcat acgggatctg cagaaactcg gccatgatga aagcggcgat
      601 ccccatcaca ggcggcatca actgaccacc ggtcgaggcg caagcttcga tggccgcggc
      661 aaaggcgggc cggtagccca ccttcttcat cagcggaatc gtgaccacgc cgatcgtgag
      721 gacgttcgac accgcacttc ccgaaatcat gccgaaaagc gacgatccga tgatggcgat
      781 cttggcgggg ccgccgcggc gacggcccat gagcgccatg gacagaccgg taaagaagct
      841 cgttccgccc accttgaaca gcaccgtgcc aaacagcgcg taaatcacca cgacggtcga
      901 aacgattttc agcggcaatc ccagcgaggc cgatgaatcc caaatgacat agtaggtcaa
      961 acgaggcagc ggtatcgatt tagccgcgag ttctccgggc aagagaccgc caacaagcgc
     1021 caggacaaag aagctcgccg tgacgatgct cagaacgatt cccgtggtgc gacgcagccc
     1081 ttcgagtatg agaaggacca atatcgtggc cacgatcaac ccatccggcg gcggcgctga
     1141 gaccagttcc gacaactgag gaaatttgac gaggacatag atcgagcagg cgcacgacgc
     1201 caatgcagcc gcaatgtcgt accaggggac gggtccgaca cgtccgccgc gaccgccgct
     1261 tgccggcacg tgcaaaaata gcaacggcat cgcaatcgcg agcaaaccgg caaggtattg
     1321 ctccgtgtaa aacacaacgc caactgcgcg gaacagatca gccgacaagc caatgacggc
     1381 gctggtcagc agaaccgcca gcatgttgat caagatcggg atactgtttg tcaatttccc
     1441 ccgctttgct ttgccggcaa ccgtcgcgct tcgggcgaac ctaacacgca actgttcgtc
     1501 attttcgggc aggcgcggac atggcacgca ttgccctgag gtctgctccc ccgcattcgt
     1561 cgcgaattcg cttcgttctg ctcctgccga aatagtcgat caagccggcc tgacggtcaa
     1621 ttgtagaaca tgtcgatcag cgccagtgac agccacggca cataggtgca cagcaccagc
     1681 accagcagca gcacgctgat gaagcctaga ttggccttgg tggtcgccca gatgtcggat
     1741 ttggcgatcg agcatgacgc aatgagcacg gaggcgaccg gcggcgtctg ctggccgatg
     1801 gcgaggttca gcgtgagcac cagaccgaaa tggatcggat caatgtgtac ctggtggacg
     1861 agcggcagca cgatcggcac cgtgaggatg atcgccgccg ccgaatgcag aaacatgccg
     1921 atgatcagga agaacacgtt gaggatcagc aacacgaaat aggggtttgt ggtgatgtcg
     1981 atgatcgaag cggcgagttt ctgcggcaac tggctttcgg tgaggtagat gccgaccagc
     2041 gccgaggccg ccaccagcag catgacgacg gcggtctgga tcgc
//
LOCUS       DASUGB010000195         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1689525, whole genome shotgun sequence.
ACCESSION   DASUGB010000195 DASUGB010000000
VERSION     DASUGB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689525"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFV87_03825"
     CDS             complement(<1..710)
                     /locus_tag="VFV87_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102912.1"
                     /translation="MAAALFGQDTAGVRLDWRRFGNLSIAGAVSAGLASGPVSRVWYS
                     ASGEGLLLTTPSGAVWETKDFETWTAAIGEAPNRVQAEASALPEAAAHVQAVGGRLYA
                     AGAYAWSSDDDGRTWRNLTQFAKFSILGGPLADLAISPVNPDDITVANANGVWRSLDG
                     GLTWDSLNALLPNLPAARLLRAPQPLRGMILGLSTGEEAEWAPGERAGWRPTKPAYTM
                     TERAFAEALAPRLGITAT"
     gene            919..1782
                     /locus_tag="VFV87_03830"
     CDS             919..1782
                     /locus_tag="VFV87_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX5102913.1"
                     /translation="MRTLRPAVWWLLAAVSLAVCPAQSQLQLPQPLPADVHYDPTFTG
                     LNESAAKVISISGQVNVLRGSQPWALNMGDFIQQKQVVLSGPDGYAIFQVSDGSTFEV
                     FPNSRVTFRNNPGAWSDLLDLWLGRVKVYVRRFGGQPNPARIYTPTAVISVRGTVFDV
                     ALEDEDTTYVGVEEGQVGVRHRLIPQDDQKLINPGESIRVYKNAPLAKARIDKGAVAH
                     RAANALAEAFYTIMTRVPRGGTGGGTPVPGGGGGAPLPGDTGAEPPPPPPPPPPPPPP
                     SGAGAPPPPGI"
     gene            1853..2485
                     /locus_tag="VFV87_03835"
     CDS             1853..2485
                     /locus_tag="VFV87_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102914.1"
                     /translation="MKCQFLLALISATGLWAQQPPAPAAGPAAGLTTEWDIQKTIHAL
                     GTHATRLIPLLNDVHPDEWISKGAPDAYIGQLNSLKAELGYAARNANELSRDAADLGK
                     AFELFSRLDASRSLFRSLTQGVRQYQNPALADLLESVELEGDAPRSRFRNYLVELASM
                     KEKELKIIDQEAQRCRGQLIRQPAARPPAQPKPKPTAAPPEPAAAKGPKP"
     gene            2482..2676
                     /locus_tag="VFV87_03840"
     CDS             2482..2676
                     /locus_tag="VFV87_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102915.1"
                     /translation="MSELKFSCSICAEPSGQICRACTKDTCDNHLCQRCLKCSDCCTC
                     DVPLTETMEAAEVSNGEPAG"
     gene            complement(2653..>3395)
                     /locus_tag="VFV87_03845"
     CDS             complement(2653..>3395)
                     /locus_tag="VFV87_03845"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX5102916.1"
                     /translation="FRVPSRDELAERLDAVLQAIYAAFTEGWADPAGADATRRDLAEE
                     ALFLARLVAELLPSEAEALGLLALILHAEARRKARRDASGDFVPLAGQDRAQWDWNRI
                     EEAECTLRQASALGAVGRFQLEAAIQSVHVARCHTGRASWAELILLYDGLIELTGSPV
                     AMLNRTLAIAEVEGAKAGLSELERLAASGRLNEYQPFWAARAELLMRAGRGAEAQAAY
                     ELAIGLESDPAVRRFLQKKQDSAGRLAV"
BASE COUNT          613 a         1236 c          941 g          605 t
ORIGIN      
        1 ctcgtcgccg taatgcccaa tctcggagcc agcgcctcgg cgaacgcccg ttccgtcatc
       61 gtgtaggccg gcttggtcgg acgccacccg gcgcgctcgc cgggggccca ttcggcctct
      121 tcgccagtcg atagccctag gatcatcccc cgcaacggct gaggcgcccg cagcagtcta
      181 gccgccggga gattcggcag cagggcattc agactgtccc acgttagccc gccgtcgagc
      241 gaacgccaca ctccgtttgc gtttgccacc gtgatgtcat ccggattcac gggcgaaatc
      301 gccaggtccg ccagcggtcc gcccaatatc gaaaacttgg cgaattgcgt caggttgcgc
      361 catgtgcgcc cgtcatcgtc gctcgaccag gcataggccc cggcggcata cagccggccc
      421 cccacggctt gcacgtgcgc ggctgcctcg ggaagggcac tcgcctcggc ctgtacccga
      481 ttgggcgctt cgccaatagc cgccgtccag gtctcaaagt cctttgtttc ccacaccgct
      541 ccgctcggag tggtcagcaa caagccttcg cccgaagccg aataccagac gcggctcacc
      601 ggcccgctcg ccagtccggc cgacacggcc ccggcgatgc tcaaattacc gaaccgccgc
      661 caatctaggc ggacgcccgc ggtgtcctgc ccgaaaagcg cggcagccat cgccacgagc
      721 aatgctccgg cgcgacaagc cgtctttttt cgagttgtgg cgccgggaca cacagtctgc
      781 accgattcta ccctttcaaa cccacatctc tatcggatgg agacggggaa aactattgat
      841 ttgatgcgtt tttgccgata gatttctggc tgggcaccta agttgcttgc acttttgatg
      901 gtagactaaa tgttcggcat gcgcactctg cgtccggcgg tgtggtggct gcttgcggcc
      961 gtttcgctgg ccgtgtgccc cgcgcaatcg caactccagc ttccacaacc acttccggct
     1021 gacgtccatt acgatcctac atttactggc cttaacgaga gtgccgccaa ggtcatctcg
     1081 atctccggcc aggtgaatgt tctgcgcggt tcgcagccct gggcgttgaa tatgggcgat
     1141 ttcattcagc aaaagcaggt cgtcctgagc ggtcctgacg ggtacgccat cttccaggtt
     1201 tccgacggca gcacgtttga ggtgtttccg aactcgcgtg ttaccttccg caacaatccg
     1261 ggcgcatggt ccgacctgct cgacctctgg ctcggtcggg tgaaagttta cgtccggcgt
     1321 ttcggcggcc agcccaatcc ggcccgtatt tacacgccga ctgcggtcat ctccgtgcgc
     1381 ggcacagtgt tcgacgttgc cctcgaagac gaagacacca cctacgtcgg cgtcgaggaa
     1441 gggcaggttg gcgtccgtca ccgtctgatt ccgcaggacg atcagaagct catcaatcca
     1501 ggcgaatcga tccgcgtcta caagaacgcg ccgctcgcca aagcccgcat cgacaaaggc
     1561 gcggtcgccc atcgtgcggc caacgcgctc gccgaggcgt tctacaccat catgacccgc
     1621 gttccgcgag gcggaaccgg cggtggcact cccgttcccg gcggtggtgg cggtgccccg
     1681 cttcccggtg acacgggcgc cgagcctcct ccaccgccgc ccccgccccc tccgccgcct
     1741 ccgccgagcg gtgccggcgc tcctcctccg cccggaatct aattccccag tcttatccga
     1801 actattgtcc gtgtcagtgt ggggcatgag acactacctg taggtgccta ggatgaagtg
     1861 tcagtttctc ctcgcgttga tatccgccac cgggttgtgg gcccaacaac cgcctgcgcc
     1921 cgctgccggc ccggcagccg gcctgaccac cgaatgggac attcaaaaaa cgattcacgc
     1981 cctcggtaca cacgcaacgc gactgattcc gctcctgaac gacgttcacc ccgatgaatg
     2041 gatctccaaa ggcgcgccgg atgcctacat cggccagttg aactccttga aagcggagct
     2101 tggctacgcc gcccgcaacg cgaacgaact cagccgcgac gccgccgacc tgggtaaagc
     2161 cttcgagctc ttcagccgtc tcgatgccag ccgcagcttg ttccgttcgc tcacccaagg
     2221 cgtccgccag tatcagaacc ccgcccttgc cgacctcctc gaatccgtcg agctggaagg
     2281 cgacgctccg cgctcgcggt tccgcaacta tctcgttgaa ctggcttcca tgaaggaaaa
     2341 agagctgaaa atcatcgacc aggaagccca gcgctgccgc ggccagctta tccgccaacc
     2401 cgccgcgcgc ccccccgccc agccgaaacc aaagcccacg gccgccccgc ccgagcccgc
     2461 cgccgcaaaa ggaccgaaac catgagcgag ctgaagttct cctgctccat ttgcgccgaa
     2521 ccctccggcc aaatctgccg cgcctgcacc aaggacacct gtgacaacca cctctgccag
     2581 cgctgcctga agtgttcgga ctgctgcacg tgcgacgtgc cgctcacgga aacgatggaa
     2641 gccgcagagg tgtcaaacgg cgagcctgcc ggctgagtcc tgcttcttct gcagaaaccg
     2701 ccgcaccgcg ggatcgctct ccaggccgat cgccaactcg taggccgcct gagcctcggc
     2761 gccccttccc gcccgcatca gcagctccgc ccgcgctgcc cagaagggtt gatactcgtt
     2821 cagccgtcca ctagccgcga gccgctccaa ctcgctgaga cccgcctttg ccccctcgac
     2881 ctccgcaatc gcgagcgtcc ggttcagcat cgccaccggc gaacccgtca actcgatcag
     2941 cccgtcgtag agcagaatca actccgccca gctggctcgc cccgtgtggc atcgcgcaac
     3001 gtgcaccgat tgaatcgccg cttctagttg aaagcgccct acggcgccca gcgcgctcgc
     3061 ctgccgcaac gtgcactcgg cctcctcaat tctgttccaa tcccattgcg ccctgtcctg
     3121 ccccgcgaga ggaacaaagt cacctgacgc atcgcgccgg gccttgcgcc tcgcctccgc
     3181 gtgcaaaatc aaagccaata aacccaaggc ctcggcttcc gatggcagca gttctgccac
     3241 cagccgcgcc aggaacaaag cttcctcggc gagatcgcgg cgtgtggcat cggcgccggc
     3301 gggatcggcc cagccttcgg tgaacgcggc atagatggcc tgcagcacgg catccaagcg
     3361 ttccgccagc tcgtcccgcg aaggaacgcg aaacg
//
LOCUS       DASUGB010000196         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_21965,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000196 DASUGB010000000
VERSION     DASUGB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21965"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFV87_03850"
     CDS             complement(<1..1195)
                     /locus_tag="VFV87_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX5102917.1"
                     /translation="MYRRRRASRLEVLISAMLKVILPDGSSKEFSSSVPVKDVAAAIG
                     PRLLKAAIAAEVDGKVVGLDFKLPEAGELKVRILTMKDSEALGVMRHSAAHVMARAVM
                     RIKKGVQLAFGPTVEGGFYYDFEMPDPLREEEFPAIEAEMKKIIDLDEPFERIEVPRA
                     QAMEIVQGLDQKYKVEHINTGLADHAQMSFYRQGEFIDLCRGPHVLSPKAIGAYKLLS
                     IAGAYWKGDANNAQLQRLYATAFFTQEQLDEHLKKIEEAKRRDHRTLGKQLSLFTISN
                     DVGPGLCLWLPKGAMIRSQLEEFIKQELARREYLPVYTPHIGRIQLYQTSGHFPYYME
                     SQFPPFYLDPFMQSVQQIFALYFQEKMSREKFIGLFEDAIKRHADGLVPVSFPGFGHA
                     KSEEEK"
     gene            1401..1474
                     /locus_tag="VFV87_03855"
     tRNA            1401..1474
                     /locus_tag="VFV87_03855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1435..1437,aa:Val,seq:gac)
     gene            complement(1608..>2348)
                     /locus_tag="VFV87_03860"
     CDS             complement(1608..>2348)
                     /locus_tag="VFV87_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX5102918.1"
                     /translation="TVDLMLIGQARVVGRSAEDVRDDLNQRYKKFYKVADINVTRLKT
                     QTRLEDLRAAVDSRFFSGGQGKRVRVTPEGTISLPSIGVVPAQGLSLDEVKAEVDARY
                     SEVVDGLEVTPILAQRAPRFIYVVGEVRNPGRFDLQGPTTVMQSIALAGGWNNGGNLR
                     QIVVFRRAEDWRLLATKIDIRGGLYGERPAPSDEIWLRDSDVVVVPKMPIQRFDDVAE
                     LIFARGIYRVLPVGFGYQLSNATTLATP"
BASE COUNT          419 a          732 c          736 g          461 t
ORIGIN      
        1 atttttcttc ctcagacttg gcgtgaccga agccggggaa cgacacggga acgaggccgt
       61 cggcgtgccg cttgatggcg tcttcgaaca ggccgataaa cttttcccga ctcattttct
      121 cctgaaagta gagggcaaat atctgctgca cgctctgcat gaacggatcg agataaaacg
      181 gcgggaactg gctctccatg taatacggaa agtgcccgct cgtctgataa agctgtatcc
      241 ggccgatatg cggcgtataa accggcaaat actcccgccg cgccagctct tgcttgatga
      301 actcctcgag ctggctgcgg atcatcgccc ccttcggcag ccagaggcaa aggccggggc
      361 cgacgtcgtt gctgatcgtg aagagcgaga gctgcttgcc gagcgtgcga tggtcgcggc
      421 gtttggcctc ttcaatcttt ttcaggtgct cgtcgagctg ctcctgcgtg aagaacgcgg
      481 tcgcatacag ccgctggagc tgggcgttgt tggcgtcccc tttccaatag gccccagcga
      541 tcgagagcag cttgtaagcg ccgatcgcct tggggcttag gacgtgcggg ccgcggcaga
      601 ggtcgataaa ttccccctgg cgatagaacg acatctgggc gtggtcggcg aggcccgtgt
      661 tgatatgctc gaccttgtat ttctggtcga gcccctgcac gatctccatc gcttgagcgc
      721 gcggcacttc gatccgctcg aagggctcgt cgaggtcgat gatcttcttc atctcggctt
      781 cgatcgccgg gaattcctct tcgcgcaacg gatcgggcat ctcgaagtcg tagtagaagc
      841 ccccttcgac cgtcgggccg aaggcgagct gcacgccctt tttgatgcgc atcacggccc
      901 gcgccatgac atgtgccgcc gagtgccgca tcacgccgag cgcctccgaa tccttcatcg
      961 tgaggatccg gaccttcaac tcgccggcct ccggcagctt gaaatcgagg ccgacgacct
     1021 tgccatcaac ctcggcggca atcgccgcct tcaggagccg cgggccgatc gccgcggcga
     1081 catccttcac agggaccgaa cttgaaaatt ccttggagct gccatccggc agaatgacct
     1141 tgagcatggc ggagatgagc acttcgaggc ggctcgcccg gcggcgtcga tacaaaggac
     1201 accgagtagc ccggcgagcg gtcgtaaagt atagccgaat cgacacttgc ggccattgct
     1261 gggttcgcct gcgctcgccg cccacgcgcg aacatcagcc gctctaacct tgtccccacc
     1321 ttgacgttag gtcgccgcag cgtagattgc tggattgaac tggatcgtcg tgccgatggc
     1381 acctccagcc tcagggttcc gggcggttag ctcagttggt tagagcgcca cgttgacatc
     1441 gtggaggtca caagttcgag tcttgtaccg cccactaatt cgtaagtcgt gccgaaatac
     1501 gtagttcggt tgcctgccgc tcaacggctt ccgatgtctc ggaatccacc gattcgcctc
     1561 gcgcgatcaa tgcagccaag tgctgatggg cacagcccat cctacggcta cggcgtcgcc
     1621 agcgtcgtcg cgttgctgag ctggtagccg aagccgacgg ggagaacgcg gtagatgccg
     1681 cgggcgaaga tcagctcggc gacgtcgtcg aagcgctgaa tgggcatctt ggggacgacg
     1741 accacgtcgc tgtcgcggag ccagatttcg tcgctggggg cgggccgctc gccatacagg
     1801 ccgccgcgga tgtcgatctt cgtagccaag agccgccagt cttcggcccg gcggaagacg
     1861 acgatctgcc gcaggttccc gccgttgttc cagccgccgg ccagggcgat cgactgcatg
     1921 acggtggtcg gcccctgcag gtcgaagcgg cccggattgc ggacctcacc cacgacgtag
     1981 atgaaccgcg gggcccgctg ggcgaggatc ggcgtgactt ccaggccgtc gaccacttcg
     2041 ctgtagcggg catcgacttc ggctttgact tcgtcgagcg acaggccctg agccggcaca
     2101 acaccgatcg acgggaggga gatcgtccct tcgggcgtga cgcgaacccg cttgccttgg
     2161 ccgccggaga agaagcggct atcgaccgcg gcccgcaggt cctccaggcg ggtctgtgtc
     2221 ttgaggcgcg tgacgttgat gtcggcgacc ttgtagaact tcttgtatcg ctggttgagg
     2281 tcgtcccgca cgtcctcagc ggagcggccc accacccgcg cctggccgat gagcatgaga
     2341 tcgacggt
//
LOCUS       DASUGB010000197         9609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_439074, whole genome shotgun sequence.
ACCESSION   DASUGB010000197 DASUGB010000000
VERSION     DASUGB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439074"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VFV87_03865"
     CDS             <1..1315
                     /locus_tag="VFV87_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5102919.1"
                     /translation="APIYNQSKRVEVVSENPTEDARRILKNFARRAFRRPVSDDDVQP
                     LVKLVEAKMAGGNSFEMAVRAGLKGVLVSPSFLFLREPPGKLDDFALASRLSYFLWSS
                     MPDEELYNLAAEGELSQPDVLRGQVERMLKDPKAQAFTENFVGQWLGLRDIDFTEPSH
                     LLYPEYDAMLRESLVKETQLFFDELLQSDLSLTNFVASDFAMLNGRLAKHYGIPDIDG
                     WEFRKVALPPDSHRGGVLTMGSVLKVTANGTTTSPVMRGAWVLDRILGTPAPPPPAEV
                     PVLEPDIRGATTIREQLAKHRQIETCAACHAKIDPPGFALESFDVIGGYREHYRTTGS
                     GETVIVDGRRMSYHRGKPVDPSDVLPDGRRFANIDELKELLLADQDQLARALTMRLLT
                     YATAARPAAADQPQIESIVNNIRDRNYGLRSLVHEIVQSDAFRSK"
     gene            1426..2694
                     /locus_tag="VFV87_03870"
     CDS             1426..2694
                     /locus_tag="VFV87_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5102920.1"
                     /translation="MKAHRRQFLQAAGVSLALPMLSAFEPRVARGAAPAGPPRRMVCV
                     CTPLGLHPANFFPQIAGKDYEATPYLELLGDYRSDMTVISGLSHPEVGSSHDSIYSFL
                     TAAPHPEQRAGFRNSISLDQFAADHIGEATRFPSLALSCEGFSLSWTRSGAIVPSDSW
                     PSSVFARLFLEGRPDEVQAQARKLADGRSILDAVADQAKTMQSGLPAGDRDKLDEYFT
                     SVRELERRLAVSEEWSKKPKPKVDVKPPVNVANPADLVGKARLMFDLIHLALQTDSTR
                     LVTVLLLGTSAVPPVPGVSQGHHDLSHHGKDPGKIEQLKLVEIEKLKTFHEFLGKLKE
                     TKEDDSNLLDRTSVFFSSNLGDASTHATKNLPVILAGGGFKHGQHLAFDPASPPPLSN
                     LYLSMLHRLGIEADNFGSSTGTLTGLEAAG"
     gene            2823..4385
                     /locus_tag="VFV87_03875"
     CDS             2823..4385
                     /locus_tag="VFV87_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HEX5102921.1"
                     /translation="MSRSRTTNGIFLIVALAAVGFLLVYIPPKVIEQYDRVRQMGPVA
                     TYVYFGLVGLGAVLLLGVSGGIVWNLWRATRQKEKRRKRGAKDPSQLSADEKQREVAD
                     NLAAVDDLQDQSAVPDDLRRELQALVDRVEQKQASQKLEIAAFGTISSGKSSLLNALA
                     GRDAFVTDPRGGTTLQRQEIPWPGDDQVLLIDTPGLGEVEGTDRVAVAAQAARDADLV
                     LLVVDGPLRESEHKLLAKLGEMEKRVLVCLNKADWYEEEEKALLLGQIMGQVRGIVDE
                     GDVIAVRAQPAVRTRVRVLTSGSETEEQVPVPADIAPLARRMLQVVRKDGRELLLANL
                     LLQSRGLVEEARRRAQEALDQRAWEIVDRYTWACGAAAALSPLPLLDLFASSALTVKM
                     VVDLGRVYRQELDLNMAVNLLAQLGKNLIAILGVNAATPAVTMAVASLLKTVPVAGTI
                     AGGALQGLVQALVTRWIGAVFIGYFQAEMKLPPEGLANLARREWQKLTSPAELLKFVQ
                     QARARSGTDRVK"
     gene            4676..5458
                     /locus_tag="VFV87_03880"
     CDS             4676..5458
                     /locus_tag="VFV87_03880"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX5102922.1"
                     /translation="MWNDDPLTFLAQINLADIAPFQLGLPTGGLLLFFFCFDQNLESV
                     RAQTGGVGRVLHVPALSLVRGRLPKRAGEPFDSCTVRYISYPSLPSAFNSDVHSLWEM
                     DFLDDDEHDAAYDGVIDEIDKALGIDKLPTHGGYNYHQLLGYPAPVQSESIEVDMERA
                     RNNLRVLPQLPVPQKSLWDEFKEFVFSCPGASQVEPPSPPPPPPPVSIDRCREWQLLM
                     EFREDEHAWIQLVDSGRLYFMYPSGEFRDGKFDHPWTAMEFY"
     gene            complement(6404..6787)
                     /locus_tag="VFV87_03885"
     CDS             complement(6404..6787)
                     /locus_tag="VFV87_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102923.1"
                     /translation="MIRLGFMALAVLMFPLAGADRSAGQGSTRLHDVVYGHKYGMGLT
                     MDVWMLHAAADPPGTMRPPVAAVPGTTSYYAGARAVNLSHYSTTCLDPSNPRLLWTCK
                     AYGHSTTDKEWCTAWAAFRLAEREP"
     gene            complement(6852..7463)
                     /locus_tag="VFV87_03890"
     CDS             complement(6852..7463)
                     /locus_tag="VFV87_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102924.1"
                     /translation="MRTLLYAGNLTDGATAAELEQLFRPHGAVVATQIIADPRSGRSL
                     GFGFVEMTAGAEAAVRELHGTQLHGRSLTVRVERRYPDDYVPDESPEMRQAVAELRER
                     AETGDPEAARELADILYFPGPFYDPQAAYKWYYIALSQQGYTVGWEDHNHTPPHYCGP
                     VGDFRNECMVSDLVVALGWERIRQLDKEAEEWMAQRNATERGT"
     gene            7636..8430
                     /locus_tag="VFV87_03895"
     CDS             7636..8430
                     /locus_tag="VFV87_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5102925.1"
                     /translation="MTTPHNQRAWDEMVAKRDRFTRPARDEDFVDPLGKVDPLGWLGG
                     DIRGKRLLCLAAGGGKHGPLYASAGAVVTVLDISPAMLELDRQVAAERRIELVTVQGS
                     MDQMPMFAAGQFDVVIHPVSTCYVPDVRPVYQEIARITRAGGLYISQHKSPVSLQTDV
                     RPSPRGYELIEPYYRTGPLPEVVGSPHREPGTLEYLHRWEELLGGMCRAGFAIEDLVE
                     PLHAKADAEIASFAHRSRYVAPYVRLKARRIGGRAEHGAALVLEPS"
     gene            8631..>9609
                     /locus_tag="VFV87_03900"
     CDS             8631..>9609
                     /locus_tag="VFV87_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5102926.1"
                     /translation="MDARLNRTALGPADVTLDATESVAGLAHDSSVCSGPQCRVHLVE
                     GSNSGRTGETEDLLRSRLRSAALLSFTGFAAFLVWHVTRVQFTSWVYVATFFDHIATT
                     FILGAIAFSLSTRCPMTTRQLRWIELVTFGVPIAFFMLLLYSKLTWWTANYSVLPEVS
                     LACWLLQIFIYALFIPNTWKRAAVVIGLMAVAPIVSLLVTMGLHADIRNLVLADPQFL
                     VEMTLVLSIATVAAVFGVYTINGLRDEVLRARQLGQYKLKRLIGSGGMGDVYLAEHLL
                     MKRPCAIKVIRPEKAGDPKVLARFEREVQATAKLSHWNSVDIFDYGRADD"
BASE COUNT         1767 a         3056 c         3001 g         1785 t
ORIGIN      
        1 cgcgccgatc tacaaccagt cgaagcgcgt ggaagtcgtc tctgagaatc ccacggagga
       61 tgcgcggcga atcctcaaga acttcgcgcg ccgggcattt cgccggccgg tatcggacga
      121 cgacgtgcag ccgcttgtga agctggtaga agccaaaatg gcgggcggca acagcttcga
      181 aatggccgtg cgggcgggcc tcaagggcgt gctcgtctcg cccagcttcc tcttccttcg
      241 cgagccgccg ggaaagctcg acgatttcgc cctggccagc cggctgtcgt atttcctctg
      301 gagttcgatg ccggatgagg agctgtacaa cctcgccgcc gaaggcgagc taagccagcc
      361 ggacgtgctg cgcgggcagg tcgagcgaat gctgaaggat ccaaaggccc aggcattcac
      421 cgaaaatttc gtcgggcagt ggctgggcct gcgcgatatc gacttcaccg agccgagcca
      481 cttgctgtat cccgaatacg acgcgatgct ccgcgagtcg ctggtcaagg agacgcagct
      541 cttcttcgac gaactgctgc agagcgatct gagcctgacg aacttcgtcg cgtcggattt
      601 cgcgatgctc aacggccggc tggcgaaaca ctacggcatt ccggacatcg acggctggga
      661 atttcggaag gtggcgctgc cgccggatag ccaccgcggc ggggtgctga cgatggggag
      721 cgtgctgaag gtgacagcca atggcacgac gacctctcca gtcatgcgcg gggcctgggt
      781 gctcgaccgc atcctcggca cgccggcccc gccacccccc gcggaagtgc cggtcctgga
      841 gccggatatc cgcggcgcga cgacgatccg cgagcagctc gccaaacacc gccagatcga
      901 aacctgcgcc gcctgccacg ccaagatcga tccgcccggc ttcgccctgg agagctttga
      961 cgtgatcggc ggctatcggg agcactatcg caccaccggc agcggcgaga cggtcatcgt
     1021 cgatggccgc cgcatgtcct atcaccgcgg caagccggtc gatccgtccg acgtgcttcc
     1081 ggacggccgc agattcgcca acatcgacga actcaaagag ctcctgctcg ccgaccaaga
     1141 ccagctcgcc cgcgccctca cgatgcgcct gctcacgtac gccacggcag cgcggcccgc
     1201 cgccgccgac caaccccaga tcgaatccat cgtcaataac atccgcgacc gcaactacgg
     1261 cctccgctcc ctcgtccacg agatcgtgca gagcgacgcc tttcgaagca agtaaccaag
     1321 gagtcttcaa ccacaaagac acaaagctca caaagagggg aattggaatg gcttccttcg
     1381 tgccctttgt ggctttgtgg ttagaaacaa gaaggacaga acaccatgaa agcccatcgt
     1441 cgccaattcc ttcaagctgc gggcgtgtcg ctcgccttgc cgatgttgag tgcgtttgag
     1501 ccgcgggtgg cacggggggc tgctccggct ggtccgccgc ggcggatggt ttgcgtttgc
     1561 acgccgctgg ggctgcatcc ggcgaacttc tttccgcaga tagcggggaa ggattatgaa
     1621 gcgacgccgt acctggaatt gctgggcgat tatcgcagcg acatgacggt catctctggg
     1681 ctgtcgcatc cggaggttgg atcgagccac gattcgatct acagcttcct cacggcggcg
     1741 ccgcatcccg agcagcgagc cggttttcgc aacagcatct cgctcgatca gttcgccgcc
     1801 gaccacatcg gcgaagcgac ccgctttccg agcctcgcgc tgtcgtgcga gggcttcagc
     1861 ctctcctgga cgcgaagcgg agcgatcgtt ccgtcggact cctggccgtc gagcgtcttc
     1921 gcccgcctgt ttctcgaagg ccggcccgac gaagtgcaag cccaggcccg caagctggcc
     1981 gacggccgca gcatactcga cgcggttgcc gaccaggcga aaacgatgca aagcggcctg
     2041 ccggcgggcg accgcgacaa gctcgacgag tacttcacca gcgtccgcga gctggagcgg
     2101 cggctggcag tctcggaaga gtggtcgaaa aagcccaagc cgaaggtcga tgtcaaacct
     2161 cccgtgaacg tcgccaaccc ggcagatctc gtcggcaagg cccggctgat gttcgatctg
     2221 atccatttgg ctctgcaaac cgattcgacg cggctggtta ccgtgttgct cctgggaacg
     2281 agtgcggtcc cgccggtgcc gggcgtctcg caggggcatc acgacctctc gcaccatggc
     2341 aaagatcccg gcaagatcga gcagctcaag ctggtcgaga tcgagaagct caagacgttc
     2401 cacgaattcc tcggcaagct caaggagacc aaggaagacg actccaactt gctggatcgc
     2461 acctccgtct tcttcagcag caacctgggc gacgccagca cgcacgccac caagaacctg
     2521 ccggtcatcc tggccggcgg cggctttaag cacggccagc acctggcgtt cgaccccgcc
     2581 agccctccgc cactctcgaa tctctacctc tccatgctcc accgcctcgg cattgaagct
     2641 gacaatttcg gcagcagcac cgggacgctg acggggctgg aagcagcggg ctaatggctc
     2701 gctcaagagt tttcaagctc gaccgacgcc gatcgaatag caatacgtta cttcgatccg
     2761 ggaagcaacc gcttgcaact gattgcggca tccgctgctg ccggtgcggt tagaatggcg
     2821 ttatgtctcg gtctcgcacc accaacggca ttttcctgat tgtcgcgctg gcggcggtgg
     2881 ggtttttgct ggtctatatc ccgccgaagg tgatcgagca gtacgaccga gtgcggcaga
     2941 tggggccggt cgcgacgtat gtctatttcg ggctggtggg attgggggcg gtgctgctgc
     3001 tgggcgtgag cggcggaatt gtctggaacc tgtggcgggc gacgcggcag aaggagaaac
     3061 gccgcaagcg gggcgcgaag gatccgagcc agctctcggc tgacgagaag cagcgggagg
     3121 tggccgacaa cctggccgcg gtggacgact tgcaggacca gtcggccgtg cccgacgacc
     3181 tgcggcgcga gctgcaggcg ctggtcgatc gcgtggagca aaagcaggcc agccagaagc
     3241 tggagatcgc cgcgttcggc accatttcca gcggcaagtc gtcgctgctc aacgccctgg
     3301 ccggccgcga cgccttcgtg accgatcccc gcggcggcac gacgctgcag cggcaggaga
     3361 ttccctggcc aggcgacgac caggttttgc tgatcgatac gccggggctc ggcgaggtcg
     3421 aagggaccga tcgcgtcgcc gtcgccgccc aggccgcccg cgatgccgat ctcgtgctgc
     3481 tggtggtcga tggcccgctg cgcgagagcg agcacaagct gctcgccaag ctcggcgaga
     3541 tggaaaaacg ggtgctcgtc tgcctcaaca aagccgactg gtatgaggag gaggaaaagg
     3601 cgctgctact cggccaaatc atggggcagg tgcgcgggat cgtggacgaa ggcgacgtga
     3661 tcgccgtccg cgcccagccg gcggtgcgga cgcgcgttcg cgtgctgacc agcggcagcg
     3721 agaccgaaga gcaagtgccg gttcccgccg acatcgcccc gctcgcccgc cggatgctgc
     3781 aggtcgtgcg caaggacggc cgcgagctgc tgttggccaa cctcctgctc caaagccgcg
     3841 gcctggtcga agaagccagg cggcgggcgc aggaggcgct cgaccagcgg gcctgggaga
     3901 tcgtcgatcg ctacacctgg gcctgcggag cagccgcggc actcagcccg ctgccgctgc
     3961 tcgatttgtt cgcctccagc gcgctgacgg tgaagatggt cgtggacctg gggcgcgtgt
     4021 atcggcagga gctggacctg aacatggcgg tcaacctgct ggcccagctc ggcaagaacc
     4081 tgatcgcgat tctcggcgtc aacgccgcga cccccgccgt cacaatggcc gtcgcctcgc
     4141 tcctcaagac cgtcccggtc gccggcacga tcgccggcgg cgcgctgcag gggctggtcc
     4201 aggccctcgt cacccgctgg atcggtgcgg tcttcatcgg ctacttccaa gccgagatga
     4261 agctcccccc tgaagggctc gccaacctcg cccgccgcga atggcaaaaa ctcaccagcc
     4321 ccgccgagct gctcaagttc gtccagcaag cgcgggcacg aagcggaacg gatcgagtaa
     4381 agtagtcgca cgatgacccg cgccgcgccc ctttccgacg agcaccgcca gttgattcgt
     4441 caggcggcga ccgcgtgtga tctcggcgat tacgctccca ggttggaggc gctagcgcgc
     4501 cccagccttg tgctggcgac gtcgcctgag ggtccgaccg gaattccgat cggtgcgacg
     4561 aagattggcg gactgccgga cgtgcccgaa gagtttagag gctaaaactt aaccgcaaag
     4621 ccctcggcag attccgagct agcagtaggt tgtgttagag cctcttagtt ggccgttgtg
     4681 gaacgacgat cccctgacat ttcttgcgca aatcaatctg gccgatatcg ctccgttcca
     4741 gcttggcttg ccgaccggag gactgctctt gtttttcttc tgttttgacc aaaacctgga
     4801 atcggtcagg gcacaaacgg gcggggtcgg tagggtcctg cacgtcccgg ctttgtcact
     4861 tgtccgcggc cgactcccca agcgcgccgg cgagccgttc gattcatgca cggtccgcta
     4921 tatttcctat ccttcgcttc cctccgcgtt caacagcgac gtccattcgc tgtgggagat
     4981 ggattttctg gacgatgatg agcacgacgc tgcgtatgat ggcgtaatcg acgagattga
     5041 taaggcgcta ggaattgaca agctaccgac tcacggcgga tacaactatc accagctcct
     5101 gggatatccg gcgccggtcc aatccgagtc aattgaagtc gacatggagc gtgcccgaaa
     5161 caacctgcgt gtacttccgc agctacctgt gcctcagaag tctctttggg atgaattcaa
     5221 agagtttgtc ttctcttgcc ctggggcttc ccaggttgaa ccgccctcgc cgcccccacc
     5281 gccgccgcca gtgagtatcg atcgttgccg cgaatggcaa ttgctcatgg agttccgaga
     5341 agacgagcac gcgtggatcc aactcgtcga ttcgggacgc ctgtacttca tgtatcccag
     5401 cggagaattt cgcgatggga agtttgacca tccctggacg gcgatggagt tctattaggt
     5461 cttgcttcct cgacatggca atacacgtct cgcggcgagt ggcccagttt gctggcaaac
     5521 ttgggccaag taaggcatcg gcgcttgatg gcttcgtttt gcgcagcgta gcccagcgaa
     5581 tcgccaaacg cgcttagcac tgctcgatgc cagtgcgatg tcacgtcggc tgggtcatgc
     5641 ttcgcaagac ccagccaccc ggtcgggcac gccctcatgt gctgccaaat gtgtagattt
     5701 cgctattcac ttccgcgacg acttcattct gcttctctcg tctgtgagtc atactccaag
     5761 tacgtgccag atcccagtga tcgaagggcc gaatacaatt cgtcgcgcaa gaggctagag
     5821 tcactttgag tgaatgaagc tataatccaa caccgtgata taagaagctt gcaacgcgca
     5881 gcgccaactc ggactaccat ttctgctcct ggaccaaatc tctccttgag tcgttcatct
     5941 gaatggatca tcgcgcgcct atttcgatcg cttgacgcaa cctttcgcgc gatctcgcag
     6001 cgctgcctaa agcgtctctc tattggatcg cctggaagta cttgcgcgga ctcgaatcgc
     6061 ctaaggaatt ctgagcaagc aagctgaaca tcaattccgt tttcgtaatc aactcggatt
     6121 tttcgcatta gtatcgcaac gggctgtgtg gccgcgttct cgcagcatat ccacgcgaat
     6181 tgccgctcgt gctacaaggt attgcggcag ggtggcaggg tttttcgcag cataacccag
     6241 cgaatcgccg ggtgcgctac cgttggccca tgccagcgcc gcccggaaca aacgtatcgc
     6301 gtgacggtcg ccccagcctg cgtcgatcgt cgcatcggct gggtcatgct tcgcaagacc
     6361 cagccacccc aacgcttgcg gacaaatgag aatgagaagc acttcatggc tcgcgctctg
     6421 ccagccgaaa cgcggcccag gcggtacacc actctttgtc ggtggtgctg tggccgtagg
     6481 ccttgcaggt ccagaggagg cgggggtttg acgggtcgag gcaggtggtg gaatagtggc
     6541 tgaggttgac cgcgcgggcg ccggcatagt agctggtggt tccgggcacg gcggcaaccg
     6601 gcggccgcat cgtgccgggc gggtctgctg cggcgtgcag catccagaca tccatcgtca
     6661 ggcccatgcc gtatttgtgc ccatagacga catcgtgcag ccgggtgctc ccctggccgg
     6721 cagagcggtc cgctcccgcg agcgggaaca tcagcaccgc cagtgccatg aaaccaagtc
     6781 gaatcatcgc gaagttctcc actgcggggc caaacgaccg cgttcaattg ctggccgggc
     6841 tgtcagcccg gtcaagtgcc gcgttctgtt gcatttcgct gcgccatcca ctcctctgcc
     6901 tctttgtcga gttgccggat tcgttcccag ccgagtgcga ccaccagatc gctgaccatg
     6961 cactcgtttc ggaaatcgcc caccgggccg cagtagtgtg ggggcgtgtg attgtggtct
     7021 tcccagccca cggtgtagcc ctgctgcgac agggcgatgt agtaccactt gtacgccgcc
     7081 tggggatcgt agaacggacc tgggaagtaa agaatgtccg cgagttcgcg cgctgcctct
     7141 gggtcgcccg tttcggcacg ctcccgcagc tccgcgacgg cctggcgcat ttccgggctc
     7201 tcgtcaggaa cgtaatcatc gggataccgg cgttccacac gcacggtcaa gctgcggccg
     7261 tgaagctggg ttccgtgcag ttcccggaca gccgcctcgg cgcctgccgt catctccaca
     7321 aagccaaagc caagcgagcg cccggacctc ggatccgcga tgatctgagt cgccacgacg
     7381 gcgccgtgcg gtctgaacaa ctgctcgagc tcagcggcag tggcgccatc cgtgagattc
     7441 ccggcgtaca agagtgtcct catgctgcga aacctaacca cgaatgaatt tgcctgctcg
     7501 ctcggtaatg ttggaacggc ttctgccggg agtcaagcaa agtgcaatct tctcgacggc
     7561 tttcggacgg gcgttcccaa cctgaagctg agaaacgaga attgaatccg caatccgcaa
     7621 tccgcaattc ccctcatgac cactccccac aaccagcgcg cctgggacga gatggtggcg
     7681 aagcgcgacc ggttcacgcg gccggcgcgg gatgaggatt tcgtcgatcc gctggggaag
     7741 gtcgatccgc tcgggtggct ggggggcgat atccgcggca agcggctgct gtgcctggcg
     7801 gcggggggcg ggaagcatgg gccgctatat gcgtcggccg gggcggtcgt gacggtgctg
     7861 gatatcagcc cggcgatgct ggaactggat cggcaagtgg cggctgagcg gcggatcgag
     7921 ctcgtgacgg tgcagggctc gatggaccag atgccgatgt ttgccgcggg gcagttcgac
     7981 gtcgtgatcc atccggtcag cacgtgttat gtgcccgacg tgcggcccgt gtatcaggag
     8041 atcgcccgca tcacgcgggc cggcggcctc tatatcagcc agcacaaatc gcctgtcagc
     8101 ctgcaaaccg acgttcgccc gtcgccgcgc ggctatgagc tgatcgagcc atactatcgc
     8161 accggcccct tgccggaggt cgtcggcagc ccgcaccgcg agccggggac gctcgagtat
     8221 ctgcaccgct gggaagaatt gctcggcggc atgtgccgcg cgggcttcgc catcgaagac
     8281 ctcgtcgagc cgctgcacgc caaagccgac gccgagatcg ccagcttcgc ccaccgcagc
     8341 cgctatgtcg cgccgtatgt ccgcctcaaa gcccggcgga tcggaggtcg agccgagcac
     8401 ggcgcagcgc tcgtgctgga gccaagttaa ttcccagcca tcgggtggtt ctctgagctt
     8461 gtaaccatcc tgcgcagaca gaatgtcctc ctggccgaat ctgtccaatt tgcctcagaa
     8521 tcgtggattc cctgcaacaa tcgacgctgg caggcgaacc ggatatgatg gacgtacgtc
     8581 ttacccagga aggaccgctg gaagcgagtt gccgctagct aggatccccc ttggacgccc
     8641 gcctcaatcg gaccgcgctc ggtcctgccg atgtcacgct cgacgccacc gaatcggtgg
     8701 cgggactggc ccacgattcg tcggtgtgca gcgggccgca atgccgcgta cacctggtcg
     8761 aagggagcaa tagcgggcgc acgggcgaga ccgaggatct gctccgctcg cgactgcgga
     8821 gtgcagcact gctcagcttt acgggattcg cggcctttct ggtctggcac gtgacccgcg
     8881 tgcagttcac gtcgtgggtt tacgtggcga cgttcttcga ccacatcgcg acgacgttca
     8941 ttctcggcgc catcgcgttt tcgctctcga cgcgctgccc gatgacgacg cggcagctcc
     9001 gctggatcga gctcgtcacg ttcggcgtgc cgatcgcgtt cttcatgctg ctactgtact
     9061 ccaagctgac ctggtggacg gccaattaca gcgtcctgcc agaagtcagc ctggcctgct
     9121 ggctgttgca gatcttcatc tacgcgctgt tcattcccaa cacgtggaag cgggctgccg
     9181 tcgtgatcgg actgatggcg gtcgccccga tcgtctcgct gctggtcacg atgggcctgc
     9241 acgcggacat ccgcaacctg gtgctggccg atccgcagtt cctggtcgag atgacgctgg
     9301 tgctatcgat tgccaccgtc gccgccgtgt tcggcgttta cacgatcaat gggcttcgcg
     9361 acgaagtgct ccgcgcccgc cagctcggac aatacaagct caagcgactg atcggcagcg
     9421 gcggcatggg ggatgtctat ctcgccgagc atctgctgat gaagcggccc tgcgcgatca
     9481 aggtgatccg ccccgagaag gccggcgatc ccaaggtgct ggcccgcttc gagcgcgagg
     9541 tgcaggccac cgccaagctc tcacactgga acagcgtcga tatcttcgat tacggccggg
     9601 cggacgacg
//
LOCUS       DASUGB010000198         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1689564, whole genome shotgun sequence.
ACCESSION   DASUGB010000198 DASUGB010000000
VERSION     DASUGB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689564"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..831
                     /locus_tag="VFV87_03905"
     CDS             175..831
                     /locus_tag="VFV87_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102927.1"
                     /translation="MSDPRRPTLERLYHRYLETENTAGFIDAVSQRYLISTLERLALG
                     GRHVTRRAAIMALGFLGNYSQNAALGRALRDRDRGVRLLADSGIRQLWRRDGSIRQQH
                     QLGRIVRLNNNEQYAAAQDAATDLIEEAPFFAEAWNQRAIGYFAQRQFEDAANDCHQT
                     LELNAYHFGAATGMAHCYLQLDEPFAALENFRRALRLNPDLEDVRGQIESLQRALEGK
                     "
     gene            complement(873..2084)
                     /locus_tag="VFV87_03910"
     CDS             complement(873..2084)
                     /locus_tag="VFV87_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5102928.1"
                     /translation="MARRKTGVWLIGAKGGVATTVTVGLTALRKQLASNCGLVSQLPE
                     FAQLDLLSWADVTLGGHEIRETTLFAEAQQLVRNNHALDGELVVKLKSDLDKLDKNTR
                     PGTLWNVGPTIEDLADRSMRNVRESPQVAIERLQADLTEFRKRNALDQVLVVNLASTE
                     PAPEVTAVPPRWKDLAKLLDKPKRCPLTASSLYAIAALDLGCPYINFTPSIGAAPAAI
                     GELALERETCHMGHDGKTGETLLKSVLAPMFAARNLQVMSWVGHNIFGNLDGKVLDDP
                     ANKRTKVKSKDRLLHQILGYSPQTLVSIEYIESLGDWKTAWDHVHFRGFLDTPMVLQF
                     TWQGCDSLLAAPLVIDLIRFTELAHRRGERGLLTFLASFFKSPLGVEENDFVRQFQML
                     QAWAATMGLVS"
     gene            2345..3538
                     /locus_tag="VFV87_03915"
     CDS             2345..3538
                     /locus_tag="VFV87_03915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX5102929.1"
                     /translation="MFDSLPLAPPDSILGLTEAFKQDPNPAKINLSVGVYKDEGGKTP
                     ILACVKQAEQRLLASEKSKGYLSIEGHAEYAARVQELLFGAGHEILRSGRAITAQTPG
                     GTGSLRVAADFLKKHFPLARVWMSRPTWANHPAIFQAAGFQVEFYPYIDAAGRGLDFG
                     GMLHALRQIPVGDIVLLHACCHNPSGIDPTPDQWRQIAHVIHEQGVLPLVDFAYQGFG
                     DGLVEDAAGLRALAQPGKELLVCSSFSKNFGLYGERVGALTLVAAGADAAQRALSQLR
                     ISIRTNYSNPPTHGAAIVATVLSDAALRQEWEQELAQMRDRIHKMRRLFVETMKRKAP
                     QHDFSFLIDQKGMFSFSGLTNLQVDELRSKHSVYVVGNGGRINVAGMTERNMNALCSA
                     IAAVI"
     gene            3659..>4994
                     /locus_tag="VFV87_03920"
     CDS             3659..>4994
                     /locus_tag="VFV87_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102930.1"
                     /translation="MRFFSRLGLVSAGLWAICVTGAVGAGPSEQQLREWAAQLDAEDY
                     DVREEATRNLTAAAEAGIDVLADVADSNSPEVAWRASEALQEIAIAGNETTLDRVVAA
                     LDRLSRTGKPGLTNIARELRAKQVRLRHDRAASKIRSFGGKLAGGGAFASDGMVVEVI
                     PELDMVIGGEVPLEIIEEVRVVEAREEVERIAVAEAPPAKPVALRALDEALKRLSDLL
                     PAGGEEELKPEEPRAEDRQTDEPPPPPPTEDISEPAPPRDLPQFPAIDAAPAEEPAPP
                     PAEAPIAEFEEVPLSLEVAGGIEIVEGEADVAIADAMVVDAGFFGGFFGGGFFVEESL
                     DLDGNPGLSTSLLLDKDWHGGDEGLAALRDLPTLYNLSIADAKLTDAALEHIAALPNL
                     HDLSVVNTKFSFEAFEKFRQQRPNTRIFARANAMLGIHADLSGACVLTGICAG"
BASE COUNT          905 a         1525 c         1601 g          963 t
ORIGIN      
        1 ccatgagttg cgcggctagc gagagttttc gcccagcacc attcttgtcg ggcgccagca
       61 ccaagtcgag cgaaaaacag ccgacttgat ttggcctgat cgttgctcgc gggaaggtcg
      121 gcgactagac tcaagtttgc cccgcctatc actcccgccg acttggagcc agccgtgagc
      181 gatcctcgcc gacccactct cgaacggctc tatcaccgtt atctcgaaac ggaaaacacg
      241 gcggggttca ttgacgcagt gtcgcaaagg tatttgatca gcacgctgga gcggctcgcg
      301 ctggggggcc ggcacgtgac gcggcgggct gcgatcatgg cgctcgggtt tttgggcaat
      361 tactcccaaa atgcggccct ggggcgggca cttcgcgatc gcgaccgggg cgtgcgtctt
      421 ttggctgaca gcggcattcg ccagctttgg cggcgcgacg gcagtattcg ccagcagcat
      481 cagttggggc ggatcgttcg cctgaacaac aacgagcagt acgccgcagc ccaggatgcc
      541 gccacggacc tgattgaaga agcgccattt ttcgccgaag cctggaacca gcgggccatc
      601 ggctactttg cccagcggca gttcgaggat gccgccaacg actgccacca gacgctagag
      661 ctcaacgcct atcacttcgg tgccgccacc ggcatggccc actgctatct gcagctcgac
      721 gagccgtttg cggccctgga gaacttccgc cgcgccttgc gattgaaccc tgacttggaa
      781 gacgttcgcg ggcaaatcga atcgctgcag cgggcgctag aggggaaata gccgagtagg
      841 acgggttttc caacccgtcc agctcgtacg ggtcaggaga ccagtcccat ggttgccgcc
      901 cacgcttgca gcatctgaaa ctgccgcaca aagtcgttct cctcaacgcc gagcgggctt
      961 ttgaagaagc tggccaggaa cgtcagcagg ccgcgttcgc cgcggcgatg ggccagctcg
     1021 gtgaagcgga tcaggtcgat caccagcggc gcggcgagca gtgaatcgca cccttgccag
     1081 gtaaactgca ggaccatcgg tgtatcgaga aagccgcgga agtggacgtg gtcccaggcg
     1141 gtcttccagt cgccgaggct ctcgatgtac tcgatgctga cgagcgtctg cggcgagtag
     1201 ccaagaatct ggtgcaggag acggtccttg ctcttgacct tggtccgctt gttggccgga
     1261 tcgtcgagca ctttcccgtc gagattgccg aagatgttat ggccgaccca gctcatcacc
     1321 tgcaggttgc gggcggcaaa catcggggcc agcacactct tgagcaatgt ttcgcccgtc
     1381 ttgccatcgt gccccatatg gcacgtttcg cgctccaggg ccaactcgcc aatcgccgca
     1441 ggtgccgcgc cgatggaggg cgtgaagttg atatacgggc agccgaggtc cagcgcggcg
     1501 attgcgtaga gagaactcgc ggttagtggg catcgtttgg gcttgtcgag cagcttcgcg
     1561 aggtctttcc accgcggcgg caccgcggtg acttcgggcg cgggctcggt cgaggcgagg
     1621 ttaaccacca gaacctggtc gagtgcattc cgcttgcgaa actcggtcag gtcagcctgg
     1681 agacgctcga ttgccacctg cggcgattcg cggacattcc gcatcgagcg gtcggcgagg
     1741 tcttcaatcg ttggaccgac gttccacagc gtgccgggcc gcgtgttttt gtcgagcttg
     1801 tcgaggtcgg acttcagctt caccaccagc tcgccgtcca gggcgtgatt gttgcggacg
     1861 agctgctggg cttcggcaaa cagcgtcgtt tcgcggatct cgtgcccacc gagcgtcacg
     1921 tcggcccacg agagcaggtc gagctgggcg aattcgggta gttgtgaaac caggccacaa
     1981 ttgctggcga gctgtttccg cagggccgtc aagccgaccg ttaccgtcgt tgccaccccg
     2041 cctttggcgc caatcagcca cacaccggtt tttctgcggg ccattcgatt gtcttgcggg
     2101 ggagggattg gagaggcggg ctgcggctgc gagggtcatc ccttcggccg gaggcaggca
     2161 agtatgtcgg ccccacggtc ctgttcgatt cgccagccgc gacgctaaga taattcgctt
     2221 tgccgggggt gtctagcagc gaacctgtgg ctgcgacaca ccgtctgatc gatgtttcgc
     2281 cgccagcgaa ccgttcgcct ggcaggctca cttctgactc ctgtcccctg ggtcctgccg
     2341 cctgatgttc gattccctcc ccctcgcacc gcccgattca atccttggtc tgactgaggc
     2401 gttcaaacag gacccgaatc ccgccaagat caacctcagc gtcggcgtct ataaggacga
     2461 gggggggaaa acacccatcc tggcatgcgt caagcaggcc gagcagcggc tcttggcgag
     2521 cgaaaagagc aagggctatc tttcgatcga ggggcacgcc gaatacgcgg ctcgcgtgca
     2581 ggagctgcta tttggtgcag ggcacgagat ccttcgctcc ggccgggcca ttacggctca
     2641 gacgcccggt gggacgggct cgctgcgtgt cgcggcggac tttctgaaga agcattttcc
     2701 gctggcccgc gtgtggatga gcaggccgac gtgggcgaat catccggcca tctttcaggc
     2761 ggctggattt caggtcgagt tctatccgta tatcgatgcg gccgggcgcg gattggattt
     2821 cggggggatg ttgcacgcac tccggcagat tcctgtgggg gacatcgtcc tgttgcacgc
     2881 ctgctgccac aatcccagcg ggatcgatcc gacaccggac cagtggcgcc agatcgccca
     2941 tgtgattcac gaacagggcg ttttgccgct cgtcgatttc gcttatcaag gcttcggcga
     3001 cggactcgta gaggacgcgg ccggcttgcg agcgctggcg cagccaggca aggagctgct
     3061 ggtgtgcagt tcgttctcga agaactttgg cctctatggc gagcgcgtgg gcgccctgac
     3121 cctcgtcgcc gctggcgccg atgccgccca gcgggcactt tcgcaactgc ggatcagcat
     3181 ccgcaccaac tacagcaacc cgccgacgca tggcgcggcg atcgtggcca ccgtgttgag
     3241 cgatgccgcc ctgcgccagg agtgggagca ggaattggct caaatgcgcg atcgaatcca
     3301 caaaatgcgg cggctgtttg tcgagaccat gaagcgcaaa gcgccccagc acgatttttc
     3361 gttcctgatc gaccagaaag gcatgttttc gttctccgga ttgaccaacc tgcaggtcga
     3421 tgagctacgg agcaagcact cggtctatgt cgtggggaat ggcggccgaa tcaacgtcgc
     3481 cggcatgacg gagcggaaca tgaacgccct gtgttcggcc attgctgcgg tcatttaggc
     3541 ccgattttcg gcccgtcgga ccgccttttt gcgcggttca tctcgacagc catgcggctg
     3601 gccgcatagt atgattatct gggatttctg cggcgattcg tgggacggag aggctgccat
     3661 gcggttcttt tcgcgactgg ggctggtttc tgccggtctg tgggcaatct gcgtgaccgg
     3721 cgccgtgggc gctgggccga gcgagcagca actccgcgaa tgggccgcgc agctcgatgc
     3781 ggaggactac gacgttcgcg aggaagctac ccgcaacctc accgcggcag cagaggcagg
     3841 cattgatgtg ctggccgacg tggccgatag caacagcccg gaagtcgcct ggcgggccag
     3901 cgaggcgctc caggaaatag cgattgccgg aaatgagaca accctcgatc gcgttgtcgc
     3961 cgccctcgac cgccttagcc gcacgggcaa gcccggcctg acgaacatcg ctcgggagct
     4021 gcgcgccaaa caggtccgcc tgcgtcacga ccgggcagca tcgaagatcc gttcgttcgg
     4081 gggaaagctg gccggcggtg gtgcgttcgc gtcggatggc atggtcgtcg aagtaatccc
     4141 agaactcgat atggtaatcg gcggcgaagt gccgttggaa atcatcgagg aagtgcgcgt
     4201 cgttgaggcc cgcgaggaag tggagcgcat tgccgtggcc gaagcgccgc ccgccaaacc
     4261 cgtcgcgctc agggcccttg acgaggcgct gaaacggctg agcgatctgt tgccggctgg
     4321 cggagaagaa gagctaaagc ctgaagaacc tagagccgaa gatcgccaaa ccgacgagcc
     4381 acctccccct ccgccgaccg aggacatatc tgagcccgca cctccgcggg acctcccgca
     4441 gtttcccgcc atcgatgcgg ctcccgccga ggaaccggct ccgccgcccg cggaagctcc
     4501 tatcgctgaa ttcgaagagg tccccctctc gttggaagtg gcaggcggaa ttgaaattgt
     4561 cgaaggcgaa gccgacgtgg ccatcgccga cgcgatggtc gtggacgccg gcttctttgg
     4621 cgggtttttc ggcggtggtt tcttcgtcga agagagcctt gatttggacg gcaatccggg
     4681 cctgagcacg tcgctgttgc tagacaagga ctggcacggt ggcgacgaag gtctggcagc
     4741 acttcgcgac ctgcctacgc tatataacct ctccatcgcc gacgcgaagc tcaccgatgc
     4801 cgctttggag cacatcgccg ccttgccgaa tctgcacgac ttgtctgtag tcaatacaaa
     4861 gttctcgttc gaggccttcg agaaattccg ccagcagcgg ccgaatacgc gaatcttcgc
     4921 ccgcgccaat gcgatgctcg gcattcatgc cgatcttagt ggcgcttgcg tgctgactgg
     4981 catttgcgcc ggct
//
LOCUS       DASUGB010000199         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_856566, whole genome shotgun sequence.
ACCESSION   DASUGB010000199 DASUGB010000000
VERSION     DASUGB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856566"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFV87_03925"
     CDS             <1..486
                     /locus_tag="VFV87_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5102931.1"
                     /translation="DKSLIQTIGRAARNVNAKVILYADKVTEAMRSAMEETSRRRAMQ
                     EKYNAEHGITPETIKKNIRAGIEEAVSAHRQANAAVGRNDDSVYITEEYVGELEEEMY
                     EAARQLDFEKAAALRDRIGQMRESMGQKLSEVQIKSYKPGPKTRKRKGGSHIPRPKKK
                     A"
     gene            581..1456
                     /locus_tag="VFV87_03930"
     CDS             581..1456
                     /locus_tag="VFV87_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5102932.1"
                     /translation="MTSRAATEYRYEKLTWPEINDAVELGKVCIVPCGAVEQHGPHLP
                     LDVDLICPGGIAKGAGQEIPDKLLVLPIVAYGYTGHVMDFPGTINNDFEHFMHHVLDI
                     TKSLAYHGFKKILLLNGHGSNMPNLDLVARRTNLETDAECICTGWWQLLSVDKNFMPS
                     WRQSKFPGGCSHACELETSLYLYLDEGNVRKDLIKSGTISFNEEDSPFMWVDLFAAGP
                     ATVVSWTSSYSETGVLGDAELATAEKGRRAYEEAVKQLVRFVTWWQPRKKDQRRDRHR
                     QPPTMPIPWGQRPLQ"
     gene            complement(1478..1933)
                     /locus_tag="VFV87_03935"
     CDS             complement(1478..1933)
                     /locus_tag="VFV87_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102933.1"
                     /translation="MPFTLQTVLSFVCMLAVLAGCSSEGPEITGLVTLDGRALSKARV
                     AFEPVTQQKAAYSAMAVCDEQGAFTVVPQSAGRTLEPGTYGVTISRKTDPQGNVPTDE
                     DYGQYEAAGALLESVPGKYTSIQPGGAYTLTVEIGKGSVEPLDLQLTSQ"
     gene            complement(1987..>2399)
                     /locus_tag="VFV87_03940"
     CDS             complement(1987..>2399)
                     /locus_tag="VFV87_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102934.1"
                     /translation="YTANSIATRGAFGNNGAATLATCIDGTSNTFAIGEAKQLHTSDA
                     YGPYWGAGTHTSVHGYVPNASYTPNYRYGACPGSAAGNRWCQYAWGFGSYHPGITQFV
                     MMDGSVQIIKDQISYAICAALCTPEGGEAAGQVP"
BASE COUNT          498 a          724 c          748 g          429 t
ORIGIN      
        1 gacaagtcgc tgatccagac gatcggccgc gcggcccgca acgtcaacgc gaaggtcatc
       61 ctgtatgccg acaaggtgac cgaggcgatg cgcagtgcga tggaggaaac gtcccgccgc
      121 cgcgcgatgc aggagaagta caacgccgag cacggcatca ctccggagac gatcaagaag
      181 aacatccggg ccggaatcga ggaagcggtc tccgcccaca ggcaggccaa cgccgccgtc
      241 ggccgcaacg acgattcggt ttatatcacc gaggaatatg ttggcgagct cgaagaagag
      301 atgtacgaag cggcccggca gctcgatttt gagaaagctg ccgcgttgcg cgaccgcatc
      361 ggccaaatgc gcgaatcgat gggccagaag ctaagcgaag tgcagatcaa gtcgtacaag
      421 ccggggccca aaacccgcaa gcgtaagggc gggtcgcaca ttccgcggcc gaagaagaag
      481 gcgtgatggc tggtgagtga gtggtgagcg gtgagtgacg gtgatatgct gccgacaact
      541 cactactcct atacacacca ctcaactgcc gaggctcgcc atgaccagcc gcgccgcaac
      601 ggaataccgc tacgaaaaac tgacctggcc ggagatcaac gatgccgtag agttgggcaa
      661 ggtctgcatc gtcccctgcg gcgccgtcga gcagcacggg ccgcatctgc cgctggatgt
      721 cgatctgatt tgccccggtg gcatcgcgaa gggagccggc caagagatcc ccgacaagct
      781 acttgtgctg ccgattgtgg cctatggcta cacgggacac gtgatggact ttccagggac
      841 gattaataac gacttcgagc acttcatgca ccacgtgctc gatattacga agtcgctggc
      901 ctatcacggg ttcaagaaga tcctgctgct caacggccac ggctcgaata tgccgaatct
      961 cgacctggtg gcccgccgca cgaacctcga gaccgatgcc gagtgcatct gcaccggctg
     1021 gtggcagttg ctctcggtcg ataagaactt catgccgagc tggcgccaga gcaaattccc
     1081 cggcggctgc tcgcacgctt gcgagctgga gacatcgctg tacctgtatc tcgacgaggg
     1141 gaacgtccgc aaggacctga tcaagagcgg caccatctcg ttcaacgagg aagacagccc
     1201 gttcatgtgg gtcgatctgt tcgctgccgg accggcgacg gttgtctctt ggaccagcag
     1261 ctattccgaa accggagtct tgggcgatgc cgaactggcc accgctgaaa aaggccgacg
     1321 ggcctacgag gaagcagtca aacaactcgt ccgcttcgtg acctggtggc aacctcgcaa
     1381 gaaggaccag cgccgcgatc gccaccgcca gccgccgacg atgccgattc cttggggcca
     1441 acggccgctg caatagtggc tttttcagag cgcgccgcta ttgcgacgtg agctgcaagt
     1501 cgagcggctc tacggacccc ttgccaatct caaccgtcaa tgtgtatgcc ccgcccggct
     1561 gaatcgacgt gtattttccc gggacggatt ccaagagcgc tccagccgcc tcatattggc
     1621 cgtagtcctc gtcagtcggg acgtttccct gggggtcggt cttgcgggat atcgtcacgc
     1681 cgtaggttcc gggctcgagc gtgcgtcctg cggattgcgg aacgaccgta aacgccccct
     1741 gctcatcgca gacggccatt gcgctatagg ccgctttctg ttgcgtcacc ggctcaaacg
     1801 caacgcgggc tttgctcaag gcgcggccgt cgagtgtcac cagtcccgtt atctccggac
     1861 cttcggacga gcacccagcc agcacggcca gcatacagac gaaagaaaga acggtttgta
     1921 aagtgaacgg catatgaagt ctcctgacga tcgagggata ggaatgcatc gccgcctgaa
     1981 ggtcagttaa ggcacttgtc ccgccgcttc gcctccttcc ggcgtacaca gggccgcgca
     2041 gatggcgtag ctgatctggt ccttgatgat ctgcaccgaa ccgtccatca tcacgaactg
     2101 cgtgatgccg gggtggtagc tgccaaagcc ccacgcgtac tggcaccagc ggttgccggc
     2161 ggcgctgcca gggcaggcgc cgtagcggta gttaggagtg tacgaagcat tgggaacgta
     2221 gccgtgcacc gacgtgtgtg tccccgcgcc ccagtacggg ccataggcat cgctcgtgtg
     2281 gagctgcttg gcttcgccga tggcgaaggt gttggaggtg ccgtcaatgc acgtggccag
     2341 cgtggcggcg ccgttgttgc cgaacgcgcc gcgggttgcg atggaattcg ccgtgtagt
//
LOCUS       DASUGB010000200         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1133787, whole genome shotgun sequence.
ACCESSION   DASUGB010000200 DASUGB010000000
VERSION     DASUGB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133787"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFV87_03945"
     CDS             <1..519
                     /locus_tag="VFV87_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchE protein"
                     /protein_id="HEX5102935.1"
                     /translation="GFPIAVEYQEREGIEWPPAPQELRVHHIENSIDTESRTFRFHLV
                     LENQWQIYQQDMHTGFLWRFRPGDRVRLQIAVEKLENVIVLPQAAVVREGPEAYVFRQ
                     NGDLFDRRAVHVLAEDRTSVVIANDGSLRPNAFVAQNGAASINRVLKAQLASGQPTNV
                     HVHADGTVHASH"
     gene            532..>3482
                     /locus_tag="VFV87_03950"
     CDS             532..>3482
                     /locus_tag="VFV87_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5102936.1"
                     /translation="MLNAVIRFALTYRLLVVVLSLTLIVYGSYLAATMPVDVFPDLDR
                     PRVVIITECPGLATEEVETLVTQPIEIALLGAAGVQAVRSQTTAGLNVIYVEFDWSTE
                     IKAARQTIQERLATLVLPDGIRPQMTPPASIMGQIVIAGMYRQRGPQGGELVPIGKTG
                     DMAELTYANGGKAQVGAWRPVDRHDVATWQPVAIEQIAWEPRGETAEALFERRALLTI
                     GGSAQEVVFPSEAEQLMDLRTTADWMVRPRLLRIPGVAEVFIQGGERKQYQILVNPTA
                     LIEYNVTLQQVETALAQSNLNTSGGFAVTGETEKPIRILGRLGPDSHRVIEDLRKIPI
                     TQDALRPVLLENVARVTEGAQFKRGDGFVNGRAGAVFTLVKQPHVDTRELTDNVAAAL
                     ADLEASLPADIVINSNLFRLKNFIDRGIFNVGEALVIGAVLVLIILFVFLLNFRTTFI
                     TLTAIPLSLVITTLVFRMLGYVTGTQLSINVMTLGGIAVAMGELVDDAIVDVENIFRR
                     LKENNASASPKPEIQVVYEASKEIRSAIVFGTAVVILVFLPLFALSGVEGRLFTPLGV
                     AYIVSILASLLVSLTVTPVLSYYLLPQSQATHREGDGPVLRVLKWAASYLIRFSMAFP
                     GWLLAATWVMVLFAAWEMANLGRNFLPQFDEGSIQVNVTLPPGSSLHASNQASRLIDE
                     KFRRRQRSSSNPDGEILHFVRRTGRAEMDEHAAPVNASEYILSMNPDMHGGREETIQE
                     LLQELRQEVPGVDIEVEQPLAHLISHMVSGVYAQIAIKVVGDDLDTLLATAEKVKTAI
                     KDVPGLTPPVIEPIQQTPELHIRLRADDLARYGLTRAYVGRIMQTALQGELAAPVLEG
                     QRRFDLLIRLEEAYRTNYANLERLRIDLPDRRGQVELGKLADIEPDGVGPNAINRENA
                     RRRIVIRCNTQDRDLASAVAEIQQRVKDRVEMPQGYFVEYGGQFESQQRATTLILLLA
                     FVSIIGMFVVL"
BASE COUNT          647 a         1083 c         1119 g          633 t
ORIGIN      
        1 gggtttccca tcgccgtgga gtaccaggag cgggagggga tcgagtggcc gccggctcct
       61 caggaattgc gcgtgcatca catcgaaaac tccatcgata ccgagtcccg cacgttccgt
      121 tttcatctcg tcctggagaa ccagtggcaa atctatcagc aggacatgca taccgggttc
      181 ctgtggcgat ttcggcccgg cgaccgcgtg cgactgcaga tcgccgtcga aaagctggag
      241 aacgtgatcg tgctgccgca ggccgcggtc gtgcgcgaag ggccggaggc ttacgtcttc
      301 cgccaaaacg gcgatctgtt cgatcggcgg gccgtacacg tgctggcgga agatcgtacg
      361 agcgtcgtga tcgccaacga cggaagcctg cggccgaatg cgtttgtcgc ccagaacgga
      421 gcggcctcca tcaatcgcgt cctcaaggcc caactagcca gcggacagcc cactaacgtg
      481 cacgtccatg ccgatggaac ggttcacgca tcccactagg agatgacgca cgtgctcaac
      541 gctgtcattc gctttgcact tacctaccgg ctgcttgtcg tcgtgctcag cctgacgctg
      601 atagtttacg gttcgtatct ggccgcgacc atgccggtcg acgtctttcc cgacctcgac
      661 cgtccgcggg tcgtgatcat caccgaatgt cccggtctgg ccaccgaaga ggtcgagacg
      721 ctcgtcacgc agccgatcga aatcgccctg ctcggcgccg cgggcgtgca agccgtacgg
      781 agccagacga cagcgggcct aaacgtgatc tacgtcgagt tcgactggag cacggagatt
      841 aaggccgcgc ggcagacgat tcaggagcgg ctggccacgc tcgtgctgcc ggacggcatt
      901 cgcccgcaga tgacgccgcc ggcgtcgatc atgggacaaa tcgtgatcgc cggcatgtat
      961 cgccagcgcg ggccgcaagg gggtgaattg gtcccgatcg gcaagacggg cgacatggcg
     1021 gagctgacct atgcgaacgg cggaaaggca caagtgggcg cctggcgccc cgtcgatcgg
     1081 cacgacgtcg caacctggca gccggtcgcg atcgaacaga ttgcgtggga gccgcgcggc
     1141 gaaactgcgg aggctctgtt cgagcggcgg gctctgctca cgatcggcgg ctcggcgcag
     1201 gaagtcgtgt ttccttcgga ggccgagcaa ctcatggacc tccgcacgac cgcggactgg
     1261 atggtccgcc cgcggctctt gagaattccg ggcgtcgcgg aagtgttcat tcaaggcggc
     1321 gagcggaagc agtaccagat tctcgtgaat cccacggcac tgatcgaata caacgtgacg
     1381 cttcagcaag tcgaaacagc gctggcccaa agcaacctca ataccagtgg cggctttgcc
     1441 gtgacgggcg aaaccgagaa gccgatccgc attctgggcc ggctggggcc ggattcgcac
     1501 cgcgtgattg aggatcttcg caagatcccg atcacccagg acgcgctgcg gcctgtgttg
     1561 ctggaaaacg tggcacgcgt cacggaaggc gcgcaattca agcgcggcga cggctttgtc
     1621 aacggccggg ccggcgccgt gttcacgctc gtcaagcagc cccatgtcga tacgcgcgag
     1681 ctgaccgaca acgtcgccgc ggccctggcc gacctggaag cctcgctgcc ggccgatatc
     1741 gtcatcaatt ccaatctgtt ccgcctgaag aattttattg accgcggcat cttcaacgtg
     1801 ggcgaggcgc tggtgatcgg cgccgtgctc gtgctgatca tcctgttcgt gtttctgctg
     1861 aacttccgca cgacattcat cacgctcacc gccattccgc tgtcgctggt gattacgacg
     1921 ctggtctttc ggatgctcgg ctacgtgacg ggcacgcagc tttcgatcaa cgtgatgacg
     1981 ctcggcggca ttgccgtggc gatgggcgaa ctggtggatg acgcgatcgt cgatgtggag
     2041 aacatctttc gccgcctgaa ggaaaacaac gcctccgcgt ctcccaagcc ggagattcag
     2101 gtcgtgtatg aagccagcaa agagatccgc agcgcgatcg tcttcggcac ggcggtcgtg
     2161 attctggtgt ttttgccgct gtttgccctc tcgggcgtgg aaggccggct ctttacgccg
     2221 ctgggcgtgg cgtacatcgt ctcgatcctg gcctcgctgc tggtttcgct gaccgtgacg
     2281 cccgtgctca gctattacct gctgccgcag tcccaggcga cgcaccgcga aggggacggg
     2341 ccggtcttgc gggtgctcaa atgggccgcc agctatctca tccgcttcag catggcgttt
     2401 ccgggctggc tcctggcggc gacctgggtg atggtgctct tcgcggcctg ggaaatggcg
     2461 aacctggggc ggaatttcct cccgcaattc gacgagggga gcatccaggt caatgtcacg
     2521 ctgcctcccg gctcgtcgct ccatgcgtcg aatcaggcgt cgcggctgat tgacgagaaa
     2581 tttcgccggc ggcagcggtc cagctccaac ccggacggcg aaatcctgca cttcgtccgc
     2641 cggaccggcc gggccgagat ggacgaacat gctgcgccgg tcaatgccag cgaatacatc
     2701 ctcagcatga atcccgatat gcacgggggc cgcgaagaga cgatccagga gctcttgcag
     2761 gagctgcgcc aggaagtccc cggcgtcgat atcgaggtgg agcaaccgct ggcccatctg
     2821 atcagccaca tggtgtcggg cgtgtatgcc cagatcgcga tcaaagtagt cggcgacgac
     2881 ctcgatacgc tgctggcgac ggcggagaag gttaaaaccg ccatcaagga cgtgccgggc
     2941 ctcacgccgc cggtaatcga acccatccag cagacgccgg aactgcacat tcgcctgcgg
     3001 gccgacgatc tggcccgcta cggactgacg cgggcatacg tcggcaggat catgcaaacg
     3061 gctctgcagg gcgagctcgc cgcgccggtg ctagaggggc agcggcgatt tgacctgctc
     3121 atccgcttgg aagaggccta ccgcacgaat tacgccaatc tggaacgcct gcggatcgac
     3181 ttgccggacc gccgtggcca ggtcgagctc ggcaagctgg ccgatattga acccgacggc
     3241 gtcgggccca atgccatcaa ccgcgaaaac gcccggcggc ggatcgtcat tcgctgcaat
     3301 acgcaggacc gcgacctggc cagcgcggtc gccgagatcc agcagcgcgt gaaggaccgc
     3361 gtggagatgc cgcagggcta tttcgtcgaa tacggcgggc aattcgagag ccagcagcgg
     3421 gcgacgacgc tgattctgtt gttggcattc gtgtcaatca tcggcatgtt cgtcgtgctg
     3481 at
//
LOCUS       DASUGB010000201         6550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1411618, whole genome shotgun sequence.
ACCESSION   DASUGB010000201 DASUGB010000000
VERSION     DASUGB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411618"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFV87_03955"
     CDS             <1..345
                     /locus_tag="VFV87_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102937.1"
                     /translation="HIAIYGQRGDQFATIRDSRWKLHVLAPRDAFATLNKPGERWIDP
                     RGPDGVTILAPYEQYQPADHPGLVSGAQSQPMQLFDLESDPGEQKDVAAEHAEIVRRL
                     KARYAEMNGSLR"
     gene            409..2721
                     /gene="eutB"
                     /locus_tag="VFV87_03960"
     CDS             409..2721
                     /gene="eutB"
                     /locus_tag="VFV87_03960"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX5102938.1"
                     /translation="MLCRRTFVKACLGGAATAALPGASRLGTAETTPLPPGVPLSAAR
                     AGEDVFAYVHRLKGAFDATLYKQILGAANPFKEGDQIVGVAAADDASRSVARTLLAGT
                     RMADLDAHPPLEDELFRLLQKSLDSTSTAQSAHLTLGELKQLLLTQPEDAIRPLMPGL
                     SSDAIGCLVKLMSDEELVAVGQKIFNPLPGSQIGAKGYLGARIQPNSPTDNVSDIQWQ
                     VFDGWAYGVGDVVLGTNPVSSDPASVAAIERTLQDLLATFGLTGVLPHCVLSHIDVQA
                     EVEKTHPGSTGIWFQSIAGSDSANATFDITLDKMLDYAGQRTGQYGLYFETGQGADFT
                     NGHDHGYDMVLHEARKYGFARALTHRVQAAQTRAGSPFSPWVHLNDVAGFIGPEVFRT
                     REQLVRCCLEDIVMGKLHGLCLGLDVCSTLHMDVSLDDLDWCLDRILPANPAYLMALP
                     TKIDPMLGYLTTGYQDHVRLREKFGYKVNDRMWQFFQELGVIDAAGRPTSRFGDPLWV
                     YLQYQRRKGDPRSDDEIRREGGQQIAAVRGRGVFLSQGHGQQPWDLEPELESDIRRIY
                     VDSKKCLWTELDPAFIERVPGLRKCDTKSRDRSDYILHPATGEQLSDESLAAVLELRR
                     KHAGKVDVQIVVSDGLNALSIIDPGHLAPFLERLRAGLVRSGFRPAPEHIVFTSGRVR
                     AGYRVGESLFAGLEGPRALLHVIGERPGTGHHTFSVYMTAVDGATWGQRGKIDHNLTK
                     VVSGIAATALAPPAGADETLRLLKTLVRQS"
     gene            complement(2745..3614)
                     /locus_tag="VFV87_03965"
     CDS             complement(2745..3614)
                     /locus_tag="VFV87_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5102939.1"
                     /translation="MKANLHHVAFLLVVFPSLALAQAQEVKRDVPYGELERQVLDIYA
                     PPNAKDVPVVFWIHGGGWQTGDKSSVQLKPQAFVEKGFVFVSTNYRLLPSVEMETIFR
                     DIAKSIRWVHDHIAEHGGDPKRILVMGHSAGAQLAALVSIDERYLKAEGLPLSIIKGC
                     VPVDGDTFDVPAIIETAETRWRVHGLPSAKFGHREKFGNDPEKHRDYSAVTHVAKGKD
                     IPPFLIVHVAGHPDTTAQAQRLASVLKEAGIPVTLYSGRETTHNKINADLGLPDDPGT
                     KALFDFVDEALKK"
     gene            complement(3707..5248)
                     /locus_tag="VFV87_03970"
     CDS             complement(3707..5248)
                     /locus_tag="VFV87_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5102940.1"
                     /translation="MNHHLSQFLRFLTAGFIVLAFVPLLPAADGPRPNVLFIISDDLN
                     NLLGCYGDERAKTPNIDRLAARGVRFERAYCTFPLCGPSRNSMLTGLYPNSTGIQANA
                     QIFRQTIPSQVSLPQAFRQAGYFAARIGKLYHYNVPKSIGTNGHDDPGSWELELNPAG
                     VDRLVEEPQIFTLTPGQFGGTLSWFASPKSDEHHTDGLNADDAEWILERCAKRKDRPF
                     FLAVGFFRPHTPYVSPKSYFELYPEKEMPVVQNVKEDQADIPPAGLASYKREQDQLTD
                     DLRRQALQAYYASISFMDAQVGRVTAALERLGLADNTIIVFTSDHGYHVGEHGLWQKM
                     SLFEESSRVPLLIVAPGVSSQAGVVQSPVSHIDLYPTLAELCGVKAPDNLQGQSLVPL
                     LKDTSVKGRGWAITQVTRGGGARRATVTTDVGSEGRRFFGYTLRTPRWRYTEWDEGEQ
                     GRELYDHDADPRELMNLADLPAHAKTVAELSQQLGTAAKSTFPPDGKTPELRPGLWAP
                     NLTDP"
     gene            complement(5395..>6550)
                     /locus_tag="VFV87_03975"
     CDS             complement(5395..>6550)
                     /locus_tag="VFV87_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5102941.1"
                     /translation="STPYSPYAWDVVLDRLPGAAAEHPKDPASYGRSTEHGIRSAQAA
                     GKPFCLVINVADPHKPFYAEGNRGETIDDPHLPSHVFMPEEVPIPGFLFDDPVVRKEL
                     AHYYSSVRRADDCVGEILQALDASGQKDNTFVMFLSDHGMPLPFAKTQLYHHSTRTPL
                     IIRWPGVVKSGAIDERHMVSAVDFLPTLLDIAGVAHPPGLDGRSFAPLVKGESQEGRD
                     TVIKEYNENAGGSRDPMRALETKQFLYVFNPWSSGERIMQTATTGTPTYRRFRELAAS
                     DPKLAARHKLYQYRVVEELYDVELDPDCLKNLIEDPAHASQCDELRKMLEGWMVKTND
                     PMLEVFRRRSDAEFREAYVQAQEREADQRRSDVGKQGKGKRKAKAKAEVD"
BASE COUNT         1189 a         2103 c         2049 g         1209 t
ORIGIN      
        1 cacatagcga tctatggcca acgcggcgac cagttcgcga ccattcgcga cagccgctgg
       61 aagctgcacg tgctggcgcc acgcgacgcc tttgcgacac taaataagcc cggcgagcgc
      121 tggatcgacc cgcgcggacc cgacggcgtt acgattcttg cgccttacga gcaataccag
      181 cccgccgatc acccaggtct ggtttccggc gcccaatccc agccgatgca gctcttcgat
      241 ctcgagtccg atccgggtga gcagaaggac gtcgccgcgg agcacgcaga gatcgttagg
      301 cggctgaagg ctcggtacgc tgagatgaat ggctcgctgc ggtaggccct ggtgccgtat
      361 cgaaggggag gacagcccga ccagaattcc tttaccggag ccagattgat gctttgccgt
      421 cgaacgttcg tcaaggcgtg tctgggaggc gctgcaaccg cagcgcttcc cggcgccagc
      481 cgcttgggga ccgctgagac aactcctctt ccaccaggcg tgccactttc tgcggcacga
      541 gccggcgaag acgtctttgc ttacgttcat cgcctcaaag gggcattcga cgccacgctt
      601 tacaagcaaa tccttggcgc ggcgaatccg ttcaaggaag gggaccagat cgtcggcgtg
      661 gcggcggcgg acgacgcttc gcgcagcgtt gcccgcacct tgctggccgg tacgcggatg
      721 gccgatctcg atgctcaccc gccgttggag gacgagcttt tccgcctgct gcagaagagt
      781 ctcgattcca catccaccgc ccaatccgct cacctgacgc tcggggaact caagcagctc
      841 ctgctcacgc aacccgaaga cgccattcgc ccgctcatgc cggggctttc cagcgatgcg
      901 attggctgcc tcgtcaagct gatgagcgat gaggagctgg tcgccgtcgg ccaaaaaatc
      961 ttcaacccgc tgcccgggag ccagatcggg gccaagggct acctgggtgc tcgcatccag
     1021 ccgaactctc cgaccgacaa cgtcagcgat atccagtggc aagtcttcga cggctgggcc
     1081 tacggcgttg gcgacgttgt cctcggcacc aaccccgttt caagcgatcc cgcgtcggtc
     1141 gccgcgatcg agcggacgct gcaggacctg ctcgccacgt tcggcctgac cggcgtgctg
     1201 ccgcactgcg ttctctcgca catcgacgtg caggcagaag ttgagaagac gcaccctggc
     1261 agcaccggca tctggttcca gagcatcgcc ggcagcgact cggccaatgc cacgttcgac
     1321 atcacgctcg ataagatgct cgactatgcc ggccagcgca ccgggcagta cggcttgtac
     1381 ttcgagaccg gccaaggggc cgacttcacc aacggccacg accacggcta cgacatggtc
     1441 ctgcacgagg cccgcaaata cggcttcgcg cgggctctga cgcaccgcgt gcaggcggcc
     1501 caaacgcggg ccggcagccc cttctcgccg tgggtgcatc tcaacgacgt ggccggcttc
     1561 atcggcccgg aagtcttccg cacccgcgag cagctcgtcc gctgctgcct ggaagacatc
     1621 gtgatgggca agctgcacgg cctgtgcctg ggactggatg tctgctcgac gctgcacatg
     1681 gacgtctcgc tcgacgatct cgactggtgc ctggatcgca tcctgccggc caatcccgcc
     1741 tacctgatgg ctctgccgac caagatcgac ccgatgcttg gctatctcac caccggctat
     1801 caggaccacg tgcgcctgcg ggaaaagttc ggctacaaag tcaacgaccg gatgtggcag
     1861 ttctttcagg agctcggcgt catcgacgcg gccggtcggc cgaccagccg cttcggcgat
     1921 ccgctatggg tctatctaca gtaccagcgc cggaaggggg accctcgcag cgacgacgaa
     1981 atccgccgcg aaggcgggca gcaaatcgcc gcggtccgcg ggcggggcgt cttcctctca
     2041 cagggtcacg gccagcagcc gtgggacctg gaacccgagc tggaaagcga cattcggcgg
     2101 atctacgtcg attcgaaaaa gtgcctgtgg accgagctcg atccagcttt cattgagcgc
     2161 gttcccggtt tgcggaaatg cgacacgaaa tcgcgcgacc ggagcgacta cattctccac
     2221 ccggcgaccg gcgagcagtt gagcgacgag tcgctggccg ccgtgctcga gctgcgccgc
     2281 aaacacgctg gcaaagtcga cgtgcagatc gtggtctccg atggcctcaa cgccctgtcg
     2341 atcatcgatc cgggacacct cgctccgttc ctggagcgac tgcgggcggg attggtccgc
     2401 agcggcttcc ggccggcgcc ggagcacatc gtcttcacgt ccggccgcgt gcgggcgggg
     2461 tatcgcgtcg gcgaatcgtt gttcgccggc ctggaaggcc cgcgagccct gctgcacgtc
     2521 atcggcgaac gccccggcac gggccaccac acgttctcgg tctatatgac agccgtggac
     2581 ggagcgacct ggggccagcg cgggaagatc gaccacaacc tcacgaaagt cgtttccggc
     2641 atcgccgcca cggcgctcgc cccgccagcg ggcgccgacg aaacgctgcg gctgctgaag
     2701 actctggtca gacaatcgta acccgaagcg taagcgaggg agcctcactt cttcagcgct
     2761 tcatccacga aatcgaacag cgccttggtg cccggatcgt ccggcaagcc gagatccgcg
     2821 ttgatcttgt tgtgggtcgt ctcgcggcca gaatacagcg tgacaggaat gccggcctct
     2881 ttcagcacgc tggccagacg ctgcgcctgg gcggtcgtat cgggatgccc ggcgacgtgg
     2941 acgatcagaa acggtggaat gtctttacct ttggctacgt gagtgacggc agagtaatcg
     3001 cgatgcttct cgggatcgtt gccaaacttt tcgcggtggc cgaatttcgc cgacggcaag
     3061 ccgtggacgc gccaccgcgt ctcggccgtc tcgatgatcg ccggcacgtc gaacgtgtcg
     3121 ccgtccacgg gcacgcagcc cttgatgatc gagaggggca gcccctccgc cttgagatac
     3181 cgctcgtcga tgctcaccag cgcggcgagc tgcgcgccgg ccgaatgtcc catgaccagg
     3241 attcgtttcg ggtcgccgcc atgctcggca atgtgatcgt ggacccagcg aatcgacttc
     3301 gcgatgtcgc gaaagatcgt ttccatctca accgacggca gcaggcgata attcgtcgag
     3361 acaaagacaa atcctttctc cacgaaggcc tgcggcttga gctggacgct cgatttgtcc
     3421 cccgtttgcc agccgccgcc gtgaatccaa aaaaccaccg gaacgtcctt cgcattgggc
     3481 ggcgcgtaga tatccagcac ctgccgctcg agctcgccgt aggggacgtc tcgcttgact
     3541 tcctgagcct gtgctagggc aagagaaggg aagacgacca gaagaaacgc aacatggtgc
     3601 aaattggcct tcatgatttc gtccccctgt cacaaaaagg gcgtgcaaaa ccaagcgctt
     3661 gacgatttgt ccaatcatcg ccactgccaa agcggctcgc aagcgtctat ggatcggtca
     3721 gattcggcgc ccacagtccc ggccgaagtt cgggcgtttt gccgtccggt gggaacgtcg
     3781 atttcgccgc cgtgccgagc tgctgcgaaa gctcggcaac cgttttggcg tgcgccggca
     3841 gatcggcaag gttcatcagc tcgcgcgggt cagcatcgtg gtcatatagc tcccttcctt
     3901 gctcgccctc gtcccactcg gtgtagcgcc accgcggcgt cctcagtgtg tagccaaaga
     3961 accgccgccc ctcggagccg acatcggtcg tcaccgtcgc ccggcgagcg ccgccgccgc
     4021 gggttacttg cgtgatcgcc cagcctcggc ctttgaccga cgtgtccttg agcagcggca
     4081 caaggctttg cccctgcagg ttgtccggcg ctttcacgcc gcacagctcg gccagcgtgg
     4141 gatagaggtc gatatggctg accggcgact gcacgacgcc ggcctggctc gaaacgcccg
     4201 gcgcaacgat caagagcggc acgcgggagc tctcctcaaa caggctcatc ttttgccaca
     4261 ggccgtgctc gccgacgtga tagccgtgat cgctcgtgaa gacgatgatg gtgttgtccg
     4321 cgagccccag ccgctcgagc gccgccgtga cacggccgac ctgcgcgtcc atgaagctga
     4381 tgctggcata ataggcctgc agcgcctggc ggcgaaggtc gtcggtgagc tggtcctgct
     4441 cgcgcttgta gctggccagg ccggccggtg gaatgtcggc ctggtcctct ttcacgtttt
     4501 gcaccacggg catttccttc tcgggataca gctcgaaata ggacttgggc gagacatacg
     4561 gcgtgtgtgg ccggaaaaag ccaacagcca gaaagaacgg ccgatccttg cgcttggcgc
     4621 agcgctccaa aatccattcg gcgtcgtcag cgttcaggcc gtcggtgtgg tgctcgtcgc
     4681 tcttgggcga ggcgaaccag cttagcgtgc cgccaaactg gcccggcgtg agcgtgaaaa
     4741 tctgcggttc ttccacgagg cgatcgacgc cggcgggatt cagctccaat tcccacgagc
     4801 cggggtcgtc gtgcccgttg gtgccgatgg acttcggcac gttgtagtga tagagcttgc
     4861 cgatccgcgc cgcgaaatag ccggcctggc gaaacgcctg cggcaggcta acctgcgaag
     4921 gaatcgtctg ccggaagatt tgcgcgttgg cctgaattcc ggtgctgttg ggatagaggc
     4981 ccgtgagcat ggaattccgg ctgggtccgc acagcgggaa cgtgcaatac gcccgctcga
     5041 agcgcacgcc gcgcgccgcc aggcggtcga tattgggcgt cttggcccgc tcgtcgccgt
     5101 agcagccaag cagattgttc agatcgtcgc taatgatgaa caacacgttt ggccgcgggc
     5161 catcggccgc gggaagcaga ggaacaaacg ccagcacgat gaagccagca gtcaggaatc
     5221 gcaagaattg agagaggtgg tggttcatgc ttggaatcct gtcattgggg tactcatgag
     5281 tagccacaga ggaaacacag atcaaacacc gatgtcaaat ggttgccttc gtcgtcgcca
     5341 cgatagactc cgtgaaaatc cgaatttcat ccgtggctcc cgcctcttgg ctgctcagtc
     5401 aacctccgcc ttagctttgg cctttcgttt gcccttgcct tgcttgccga cgtcgctgcg
     5461 acgctggtcg gcttcgcgtt cctgggcctg gacgtaggct tcgcgaaact cggcgtcgct
     5521 acggcggcgg aagacctcca gcatggggtc gttggtcttc accatccagc cctccagcat
     5581 ttttcggagc tcatcgcact ggctggcatg agccgggtct tcgatcaggt tcttcaggca
     5641 atccgggtcg agttcgacat catacagttc ctcgacgacc cggtactgat agagcttgtg
     5701 ccgcgccgcc agtttggggt cgctcgccgc cagttcgcgg aagcggcgat aggtcggcgt
     5761 gccggtggtg gcggtctgca tgatccgctc gccgctcgac cacggattga agacatacag
     5821 gaattgcttc gtttcgaggg cccgcatcgg atcgcgcgag ccgccggcat tctcgttgta
     5881 ttccttgatc accgtgtcgc gcccttcctg cgactcgccc ttaaccagcg gggcgaacga
     5941 acggccgtca agccccggcg gatgagccac gccggcgatg tcgagcagcg tcggtaggaa
     6001 atcgactgcc gagaccatgt gtcgttcgtc gatcgcgccg ctcttgacga cgcctggcca
     6061 gcggatgatc agcggcgtcc gtgtgctgtg gtgataaagc tgcgtcttgg cgaacggcag
     6121 gggcatgccg tgatcggaga ggaacatgac gaatgtgttg tctttctggc cggaggcgtc
     6181 gagcgcctgc aggatttcgc ccacacagtc gtccgctcgc cgcaccgacg agtagtagtg
     6241 tgccagctcc ttccgcacca ccggatcgtc gaacagaaat cccggtatcg gcacctcctc
     6301 aggcatgaaa acgtgagatg gcaagtgcgg atcgtcaatc gtctcgccgc gattcccctc
     6361 ggcatagaag ggcttatgcg ggtcggccac gttgatcacg aggcagaacg gcttgccggc
     6421 ggcctgcgcc gagcgaatgc cgtgctcggt ggagcggccg tacgaagcgg gatccttggg
     6481 atgctcggcc gccgcgccgg gcaggcggtc gagcacgacg tcccaagcat agggcgagta
     6541 gggcgtcgaa
//
LOCUS       DASUGB010000202         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1829540, whole genome shotgun sequence.
ACCESSION   DASUGB010000202 DASUGB010000000
VERSION     DASUGB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829540"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFV87_03980"
     CDS             complement(<1..296)
                     /locus_tag="VFV87_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5102942.1"
                     /translation="MPRAITTTDITGQFILLGTGTSVGVPSIGCPCEVCASTDPKDKR
                     TRCAALVGLPQGNLLIDTPPDLRQQLLREKVGIAHAVLYTHEHADHLFGLDDLRL"
     gene            444..776
                     /locus_tag="VFV87_03985"
     CDS             444..776
                     /locus_tag="VFV87_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5102943.1"
                     /translation="MSAAVQYPHISLDTQGQARIGSTRYKVLHLAGEHFHYGWSAEEL
                     LRQHPDLKPEEVYAALTYFYDHYDAMCDALRSTGQKAAALRDEALQSGRLLSRDELLR
                     RQKLTEAG"
     gene            776..1060
                     /locus_tag="VFV87_03990"
     CDS             776..1060
                     /locus_tag="VFV87_03990"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5102944.1"
                     /translation="MLRLYMDVHVPAPITESLRRRGIDVLTSQEDGTRTAADESLLQR
                     ATALGRILVSQDEDLLNLAAQWQTTSLEFAGLIFAPQEGGSMGFMWTTWN"
     gene            1129..1668
                     /locus_tag="VFV87_03995"
     CDS             1129..1668
                     /locus_tag="VFV87_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102945.1"
                     /translation="MSPTSKSKTPACRRFWPRFTLGVLLVAVTVVGIGLGIWTHRARD
                     QRRIVKHIRETSGDVAYAWEPHVSPQHGTVSSPVPAWLLDRLGEDFFHSVDRAYGGNE
                     VDLAQVSRLTHLRVLEIGKSDLTDSELKCVTRLHSLKELIIHQDQRNPQYGGTTKITN
                     RSLALLAQMSQLEGGIARW"
     gene            1975..>2172
                     /locus_tag="VFV87_04000"
     CDS             1975..>2172
                     /locus_tag="VFV87_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102946.1"
                     /translation="MSLTSELPAPTRRRFWPRFTLRVLLVAVTAVGVGLGIWTHRARE
                     QARIVKLIQDGGGMVCYERDGP"
BASE COUNT          418 a          665 c          675 g          414 t
ORIGIN      
        1 agccgcaggt catccagtcc gaacaggtgg tcggcgtgct cgtgcgtata gagcacggca
       61 tgggcaatgc ccaccttctc ccgcagaagc tgctgccgca gatcgggcgg cgtatcgatc
      121 aggaggttcc cctgcggtag gccgaccagc gccgcgcagc gggtccgctt gtccttgggg
      181 tcggtgctgg cgcaaacctc gcacgggcag ccgatcgacg gcacgccgac gctcgtgccg
      241 gtccccagca agatgaattg gccggtgata tcggtcgtcg taatcgcgcg cggcacgcta
      301 aactccaggg cgggtgtctc gccccagatt ggcacagccc cgccaatgtg acaagtccgc
      361 cgccggcgtc taagatgcag gcaattgccg acgcgctcga cggtgtggag tagaattaga
      421 atcatcgcac tgagggtcgc cgcatgagtg ccgctgtcca atacccccac atttcgctcg
      481 atacccaggg ccaggcccgg atcggcagca cccgctacaa ggtgctgcac ctcgccggcg
      541 agcacttcca ctacggctgg tccgccgaag aactcctgcg gcagcatccc gacctcaagc
      601 cggaagaggt gtatgccgcg ttgacctact tctatgatca ttacgacgcg atgtgcgacg
      661 cgctgcgttc cactggccag aaggcagcgg cgctccggga cgaggcgctg cagtccggcc
      721 gcctgctatc ccgcgatgaa ttgctccgcc gccagaaact gacggaggcc ggctaatgct
      781 gcggctgtac atggacgtgc atgtacccgc gccgatcacc gagagcctgc gccgccgcgg
      841 cattgacgtt ctgaccagcc aggaggacgg cacccgcacc gctgccgacg aatcgctcct
      901 tcaacgggcc accgctctgg gccgaatcct cgtcagccag gacgaggatc tgctcaatct
      961 ggcagcacag tggcagacga ccagcctgga attcgctgga ctgatcttcg ccccgcagga
     1021 aggcggcagc atgggcttta tgtggacgac ctggaattga tcgccggttg ctgctcagat
     1081 gaagagctgc ggaacacggt ctattacttg ccgctggcgt aatgctcgat gtcaccaacc
     1141 agcaaatcga agactcctgc ttgccgccgc ttctggccgc gattcacgct gggggttctt
     1201 ctggtcgcgg ttacggtggt cggcatcggc ctcgggatct ggacccaccg cgcccgcgat
     1261 cagcgacgaa ttgtcaaaca tatccgggaa acgtcgggcg atgtcgcgta tgcctgggag
     1321 cctcacgtat caccacagca cggaacagta agctcgccgg tcccggcttg gttactcgat
     1381 cgcttgggcg aggacttctt tcacagcgtt gaccgcgcgt atggagggaa cgaggttgac
     1441 ctggcgcagg tttcgcgact gacgcaccta cgcgttctgg agatcggaaa atcggatctg
     1501 acggattccg aactgaaatg cgttacacgc cttcatagcc tgaaagaact gatcattcat
     1561 caagatcaac gaaatccgca atacggaggg acgacgaaga taaccaatcg ttcgctcgcg
     1621 ctgctcgccc agatgtctca gctggaaggg ggtattgctc gatggtgatc ggtttacggc
     1681 agttggcatg gcggctttgg cccaatcacg ctccttgaga tttgtggcgc tcaactattg
     1741 tgatgaacac ttgttggagg acgcagttga gccattccat cgcgccgggc gtgttattga
     1801 ggactgcgaa accaatgcgc caactccgac ccccgaaagt ccctggtttt cacgtacgaa
     1861 cgctagcggg gtcagggttc attggcggcg cagtcgtcag tagttacttg gtcgtacgaa
     1921 agcggtctag cgatggagta caagtgacgc tcgtggaaga agggtcgccc tttgatgtca
     1981 ctaacaagtg aattgccggc tccgactcgc cgccgcttct ggccgcggtt cacgctccgt
     2041 gtgcttctgg tcgcggtgac agcggtcggc gtcggcctgg gcatctggac ccaccgcgcc
     2101 cgcgagcagg cgcggatcgt gaagctgatc caagacggcg gcggcatggt ctgctacgag
     2161 cgcgacggac cc
//
LOCUS       DASUGB010000203         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1829692, whole genome shotgun sequence.
ACCESSION   DASUGB010000203 DASUGB010000000
VERSION     DASUGB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829692"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFV87_04005"
     CDS             <1..962
                     /locus_tag="VFV87_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102947.1"
                     /translation="RSLVLAVLVAALVPVLAVLAQTKGPRPVTRARPPKLEKSNNFYN
                     DAFTEGLVGQRPADLGKKPAAGGGEAAPGSARSGGSGTGGVAGSGWAALISGATIEDE
                     IKSLKLRVDQNVTTPSDFAGKGYKLARRDFSVLAMLFAIAGEYEGDVRWKSDAPAARD
                     AFARTANNAKVGTQQVWQEAKQRKDELTDMVGGSSPFSGKNAEAKATWKDVCGRAPLM
                     QHLESVWEPKLKPLLADKGQFTANSEEVVRHAEMIAAIGHVLEKEGMPDADSEEYVKF
                     GRSLRDGAKAIVEAAKSKNFDQATGGGTAIGKSCTECHENYKA"
     gene            complement(982..1869)
                     /locus_tag="VFV87_04010"
     CDS             complement(982..1869)
                     /locus_tag="VFV87_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5102948.1"
                     /translation="MKILSWLGFAAMICLLAVLAPARAWALGEEQVGNEPLSAANYVK
                     WPGLAEVVNDRARAYYFWVNGNENFYYVGSVAELNAALKNFAAAELKEHEVLLRPDTG
                     TVQSFQGDNKFTFNWHLQIMDGIAGHLTTLEKGDQVWNKHPRLTIHVSGDVALDKLDI
                     PKGVKLLTVSDLSARARQGIESKDKTVRGWCAGVIADLNPRDAENQAALEKLLADEDD
                     WVRLNAAGSIPLFGTKAKSALPALKGCLERKDEGLKTRAQKAIETIESAQEDAAADRA
                     HAETMERIKKFVADHKANP"
     gene            complement(1986..2774)
                     /locus_tag="VFV87_04015"
     CDS             complement(1986..2774)
                     /locus_tag="VFV87_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102949.1"
                     /translation="MDKQSVTAFFLALITILASIFWALPHIAPAQENVPIAPVTALAR
                     RLDIEFNETRLVDAVDYLQQQTGANFYLRARKLEEAGVNLDTPISSQFRAIRLDTWLD
                     LALDDIDLTWMVKDDLIVITSPEDAERHLVTRVYDCRDLLALPRPTITIPERTTPDEA
                     KAGQPTGDSSDKEKSAACQFCGSVRYVPKRPADPAEHLMEVITQAIDLHLWDENGGPG
                     TIGEYSGMLVVTQTDRVHGKVERFLNMLREAAGLEVVGVKKVVR"
     gene            complement(2832..3077)
                     /locus_tag="VFV87_04020"
     CDS             complement(2832..3077)
                     /locus_tag="VFV87_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102950.1"
                     /translation="MGPAAPLTEHEKRAQSLIALITTNVDPDRWSEMGGPGSISEYHG
                     LIVVTQTAQTHNKIERVLDMLREAAGLEVGGVKKVVR"
BASE COUNT          617 a         1080 c         1000 g          632 t
ORIGIN      
        1 gccgctcgct cgtgctggct gttctcgttg ctgccctggt cccagtcctg gccgtcctcg
       61 cccagacgaa ggggccgcgg ccggtcacgc gggccaggcc gccgaagctc gagaaaagca
      121 acaacttcta caacgacgcc ttcaccgaag ggctcgtcgg ccagcggccg gccgacctgg
      181 gcaaaaagcc ggcggccggg ggcggcgaag ccgcgccggg cagcgccaga tccggcggca
      241 gcggcacggg cggcgtggcc ggatcgggct gggcggcttt gatctcgggc gccacgatcg
      301 aggacgagat caaatcgctc aagctgcggg tcgatcagaa cgtgacgact cccagcgatt
      361 tcgccggcaa aggctacaag ctggcccgcc gcgatttcag cgtgctggca atgctcttcg
      421 cgatcgccgg cgaatacgaa ggcgacgtcc gctggaagag cgacgccccg gcggcccgcg
      481 acgccttcgc ccgcaccgcc aacaacgcca aggtggggac gcagcaggtt tggcaagaag
      541 ccaagcagcg gaaggacgag ttgaccgaca tggtcggcgg cagctcgccc ttcagcggca
      601 aaaatgcaga ggccaaggcg acgtggaaag acgtttgcgg ccgcgccccg ctgatgcagc
      661 acctggaatc ggtctgggag ccgaaactta agccgctcct ggcagacaag ggccaattca
      721 cggccaatag cgaggaagtt gtccgccacg ccgaaatgat cgccgccatc ggccatgtgc
      781 tggagaagga aggcatgccc gacgccgact cggaggaata cgtcaaattc ggccggtcgc
      841 tccgcgatgg ggccaaggcc atcgttgaag ccgccaagtc gaagaacttc gatcaggcca
      901 ccggcggcgg cacggccatc ggcaagtcct gcaccgagtg ccacgagaat tataaggcgt
      961 agcgcgggcg agacctgggc tctagggatt ggctttatgg tccgcaacga atttcttgat
     1021 tcgctccatt gtctcggcgt gcgcgcgatc ggcggccgcg tcttcctgag ctgactcgat
     1081 ggtctcgatt gccttttgag ctctggtctt cagaccttca tccttgcgct cgaggcaccc
     1141 cttgagcgcc ggaagcgcgg atttggcttt cgtgccaaag agcgggattg agccggccgc
     1201 gttgagccgc acccagtcgt cttcgtcagc cagcagcttc tctagtgccg cttgattttc
     1261 ggcgtcgcgc ggattcagat ccgcaatcac gcccgcgcac cagccgcgga cggtcttgtc
     1321 cttgctttcg atcccctggc gagcacgggc cgagagatcg ctgaccgtga gcaacttcac
     1381 gcccttggga atatccagct tgtccagcgc gacatcgccg ctgacatgaa tcgtcagtcg
     1441 cgggtgcttg ttccagacct ggtcgccctt ttccagcgtg gtcagatgcc ccgcgatgcc
     1501 atccatgatc tgcaggtgcc aattgaacgt gaatttgtta tcgccctgga agctctgcac
     1561 cgtccccgta tccggccgca agaggacctc gtgttctttc aactccgccg ccgcgaagtt
     1621 cttcagcgcc gcgttcagtt ccgccacgct gccgacgtaa tagaaattct cgttcccgtt
     1681 aacccaaaag taatacgccc gggcccgatc attgacgacc tctgccagcc ctggccattt
     1741 gacgtaattc gccgccgaga gcggctcgtt gccgacctgc tcttcaccca aggcccaggc
     1801 ccgcgcgggc gcaagcacag ccagcagaca gatcattgcc gcaaatccaa gccacgacaa
     1861 gattttcatc gaatcgcctc cggcaaagaa atagggacgt ccgctgacca actatagacg
     1921 aaacgtctat agcaatggat tgcggctgtt tcccatattc cggaatcaag taacagggcg
     1981 atggactacc gcaccacctt tttcacgccg acgacctcca acccggctgc ttcgcggagc
     2041 atattcagga atcgttcgac cttgccgtgc acgcggtcgg tttgcgtgac gaccagcatt
     2101 ccgctgtatt cgccgatcgt ccccggccct ccattctcgt cccagaggtg cagatcgatg
     2161 gcttgcgtga tgacttccat caggtgctcc gccggatcgg ccggccgctt ggggacgtac
     2221 cgcaccgatc cgcagaactg acaggctgcc gatttttcct tgtcgctcga gtctccggtc
     2281 ggctgaccgg cctttgcctc atcgggcgtg gtgcgctcgg gaattgtgat tgtcggtctt
     2341 ggcagcgcca gcaaatcgcg gcagtcatag acgcgcgtca ccaggtgccg ctcggcatct
     2401 tcgggcgagg tgatcacaat caggtcgtcc ttgaccatcc aggtcaggtc gatgtcgtcc
     2461 aaggccaggt ctagccacgt gtcgaggcga atcgcccgga actggctgga aataggcgta
     2521 tccaggttca cgcccgcctc ttcgagtttg cgggcgcgga gatagaagtt cgcccccgtt
     2581 tgctgttgca aatagtccac cgcatcaacc aggcgcgtct cgttgaattc gatatccagc
     2641 cgccgcgcca gtgccgtcac tggcgcgatc ggcacgttct cttgagcagg tgctatgtga
     2701 gggagcgccc agaaaatact cgcaagtatc gttatcaacg ccaggaagaa ggccgtcacc
     2761 gattgcttgt ccatgatttc acctcgggcg aacgcacttg tgcccctgtg ccatggctgg
     2821 tccacgcaac gctacctcac gaccttcttc acgcccccca cctccagtcc ggccgcttcg
     2881 cggagcatat cgaggacgcg ctcgatcttg ttgtgggtct gggcggtctg cgtcacgacg
     2941 atcaggccgt gatactcact gatcgatccg gggcctccca tttcgctcca acgatcggga
     3001 tcgacattgg tcgtgatcag cgcgatcagc gactgggccc gcttttcgtg ctcggtcagc
     3061 ggcgccgctg gtcccatgtc tccaccgcca aggcctggaa cacgtccgcc aagtcctccg
     3121 tcaccgccga acccgccgcc tcccgcgccg ggcgcgaatt gcggcagaat ctcgcgcgga
     3181 atggttgaag ctcgcgacga ctcgaatggc gttcgacccg ggcctgcggt ctcgtcattg
     3241 tcaggaatct gcaaggggct cccggttggc gacggaaaag aacctggacc cggcaccttg
     3301 gggaggaccg gccgcggaat atagtcggc
//
LOCUS       DASUGB010000204         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_161711, whole genome shotgun sequence.
ACCESSION   DASUGB010000204 DASUGB010000000
VERSION     DASUGB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161711"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VFV87_04025"
     CDS             <1..1430
                     /locus_tag="VFV87_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102951.1"
                     /translation="GPFEITPATMHQLAPQPGAAMNRLLIWPVQAIDGPAHLQIRSPL
                     QSLPGQTLRAPNVLLTDAPELERVVVLATRMDSQPVVWETRGLQAAQPQSLRLPNGWM
                     QEQEEAYRAARDEFDATASFPARTQTRPVAKLAQIEIDRREGSRWLGKATYYLAPAGA
                     AEVIVEMPPNTRLAQLSIDGAAAQCRPTGLRGWRVPTNSRRIGTRLEVLFECAGARDG
                     ATGMPVVDAPRLRGVAVDRTLWRLRGLAVPGASDPALRAADELEAGLILAETSADQLR
                     QLSQIQSGDLPPAIFRATYSQWHDLYDGQQASLIALAAVKPPSEKQASRLRAAAEFVA
                     QADQRISQSGAWSPPAAGASTESDSSADTLAADGITTLIADGWQPSMAVQTLYPRRSP
                     EGFPLGIATAALLLTGLGLVLRWHGAVDWLAAHAPAVLAIAGLAWWLLAPLAWLGWAF
                     VALAAWLAFRSPWAGRAVTLNRIRR"
     gene            1461..2471
                     /locus_tag="VFV87_04030"
     CDS             1461..2471
                     /locus_tag="VFV87_04030"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5102952.1"
                     /translation="MSQLPPLPTACIPATFDAIRRAAGVLRAGGLVAFPTETVYGLGA
                     AAENSQAVRAMFAAKGRPANHPVIVHLADVSQVDEWALEVSGQVRALAAAFWPGPMTL
                     VVRRTRRASDLVTGGLDAVGLRVPGHPIAQALLQEFGGAVAAPSANRFGQVSPTLARH
                     VMEELAGRVDLVLDGGPCTVGLESTIIDLTGPQPAILRPGGIAAEQIEAVTGPVLGGR
                     NDQPRVSGSLPSHYAPRARIEVVRADEVVARASTLAKTGARVAVLGPSTLAVPEGIIL
                     LALADDLAAAAKSLFETLRRVDELGCDVGVTSLPAARGLGVAIADRLIKAAGPRYDDP
                     IM"
     gene            2561..3514
                     /locus_tag="VFV87_04035"
     CDS             2561..3514
                     /locus_tag="VFV87_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5102953.1"
                     /translation="MDRTSSAVSRRAFTLVELLVVIAIIGILVALLLPAVQAAREASR
                     RTSCSNNLKQIGHALHNHHDVHGFLPPGSIQVAAAAQPAHERLNIPLTVNHGWGAFLF
                     PFIEQKGLYDQYRFDLDWRDPLNQPARSQFLAMMNCPSTPDRQRIDNHTQDGFTYSVK
                     PGDYGVMSNVSGGTLYPLGLVDKATDESEAGIMQTNRLQTLADMRDGTSKTIMMVEDA
                     GRHQRFVAKTRRINGRYSGSSCLDHENIMTLHGYDNTGTSTPGPCPLNCTNNNEIYGF
                     HPGGAQVVMGDGAVRMLTAGMPLRLVARLVTRGAGDNGGNE"
     gene            3542..4021
                     /locus_tag="VFV87_04040"
     CDS             3542..4021
                     /locus_tag="VFV87_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102954.1"
                     /translation="MEIAGVNLWRARHSIGAGLLLIGLLHVSGCGSGGPPRKTCFPVR
                     GQLFVADQPADGAMIYFQPQDGSPPEEWTSGYPRARVAADGSFEVSTYADRDGAPAGD
                     YVLLVQWIPGAANDEGEPSDDESSGDRLGGRFMDPASSPLRAIVGPEPTTLERIDVR"
BASE COUNT          721 a         1360 c         1405 g          750 t
ORIGIN      
        1 cggggccgtt tgagatcacg ccggccacga tgcaccagtt ggcgccacag cccggtgcag
       61 cgatgaatcg attgctgatc tggccggtgc aagccatcga cggtccggcc cacctgcaga
      121 ttcgctcgcc gctccagtcg ctcccagggc agacgttgcg ggcccccaat gttctgctga
      181 cggatgctcc agaactggag cgagtggtcg tgctcgccac gcggatggat tcgcagcccg
      241 tcgtgtggga aacgcgcggc ctgcaagcag cccaaccgca atcgctccgg ctgcccaatg
      301 gctggatgca agagcaggaa gaggcatacc gtgcagcgcg ggatgaattt gatgctaccg
      361 cttcattccc ggccaggacg caaacgcgcc cggtcgccaa gctggcccaa atcgagatcg
      421 accgccgcga gggctcccga tggctgggca aggcgacata ctacttggcg cccgccggcg
      481 cggcggaggt cattgtcgag atgcctccga acacacgact ggcgcagctt tcgatcgacg
      541 gcgctgccgc ccagtgccgg cccacgggtc tgcgcggatg gcgcgtgccg acgaattcgc
      601 ggcgcatcgg tacccgcttg gaagttctgt tcgagtgcgc tggcgctcgc gatggtgcga
      661 ctggcatgcc ggtcgtggac gcgccgcgac ttcgaggagt ggccgttgat cgcacgctct
      721 ggcgtcttcg cggcctcgca gttccagggg cgagcgatcc ggcgcttcgc gcggcggatg
      781 agctggaagc agggctgatc cttgcggaaa catcagccga ccagcttcgg cagctctcgc
      841 aaatccaatc gggggatctg cctcctgcga tctttcgtgc gacctactcg cagtggcatg
      901 atctgtatga cgggcaacag gcaagcttga tcgcccttgc agccgttaaa ccgcccagcg
      961 agaagcaagc cagccgactg cgtgccgcgg cggagttcgt cgcgcaagcc gaccagagga
     1021 tcagccagtc cggcgcttgg tcgccgcccg ccgcgggcgc ctcaaccgaa agcgactcga
     1081 gtgctgacac tctggcggct gacggcataa cgacgttgat cgcagatggc tggcagccgt
     1141 cgatggccgt gcagacctta tatccgcggc ggtcgccaga ggggtttccg ctgggaatcg
     1201 ccacagccgc cctgttgctg acgggattgg ggctcgtcct gcgctggcac ggtgcggtcg
     1261 attggctggc cgcccacgcg ccggccgtgc tggcgatcgc cggcctcgcc tggtggctgc
     1321 tcgcgccgct ggcctggctg ggctgggcat ttgttgcctt ggcagcctgg ttggcgtttc
     1381 gctcaccgtg ggccggccgc gccgtaacgc tgaatcgcat tcgccgctaa ccgccgccac
     1441 ggaacagttc aatctgtcgt atgtcccaat tgcctccgtt gcctaccgca tgcattccgg
     1501 ccaccttcga tgcgatccgt cgagcggcag gcgtgctgcg tgcaggcggg ctggtcgcat
     1561 ttcccacaga aacggtctat ggactgggcg ccgctgcgga gaattcccag gcagtccggg
     1621 cgatgtttgc cgccaaaggt cgtccggcaa atcatccggt catcgtccac ctggccgacg
     1681 tctcgcaagt cgacgagtgg gcactcgaag tcagcggaca ggtgcgggca ctcgcggccg
     1741 cgttctggcc cggacccatg accctcgttg tgcgccgtac cagacgcgcc agcgacctgg
     1801 tgaccggtgg actcgacgcg gtcggactgc gcgtgccagg tcatcccatc gcccaggctt
     1861 tgctgcagga attcgggggc gcggtcgcgg ccccatcggc caatcgattc gggcaggtga
     1921 gtccgacgct ggcccggcac gtgatggagg aattggccgg gcgcgtggat ctggtgctgg
     1981 acggcggtcc gtgcacggtc ggtctcgaat cgacgattat cgatctgacc ggaccgcagc
     2041 cggccatctt gcgtcccggt ggcattgcgg cggagcagat tgaggctgtg accgggccag
     2101 tgctcggtgg acgcaatgat cagccgcgag tctcgggcag ccttcccagc cattacgcac
     2161 cccgcgcacg cattgaggtg gtgagggctg acgaggttgt cgctcgggcg tcaacgttgg
     2221 ccaagaccgg tgcgagagtc gcagttctcg gcccaagcac tttggctgtg ccggaaggga
     2281 taattctcct ggcgctggca gacgaccttg ctgcggcggc gaagtctctt tttgagacgc
     2341 tccgccgcgt cgatgaactg ggctgcgacg ttggggttac cagtcttcct gccgcccgcg
     2401 gccttggcgt cgccatcgcc gaccgcctga tcaaagctgc cggaccgcgg tacgacgatc
     2461 ccatcatgta aagagaaatc ttgatgagga ttgctggcgt tttcggggcg caccagggag
     2521 aataccgcct tcgttcttcg gagcctttgt gagccaaagc atggaccgca catcgtccgc
     2581 tgtaagccgc cgcgccttca cgctcgtcga gctattggtc gtgatcgcga ttattggaat
     2641 tctggttgcc cttctgctgc cggccgtgca ggcggcgcgg gaggccagcc ggagaacttc
     2701 gtgctccaat aacctgaagc agatcggcca cgcgctgcac aaccaccacg acgtgcacgg
     2761 gttcctgccg cccggatcaa tccaagtcgc tgcagcggcc caaccagcgc acgagcggct
     2821 taatattccg ctcacggtca atcacggctg gggggcgttt ctcttcccgt tcatcgagca
     2881 gaagggtctc tacgaccagt accggtttga tttggactgg cgcgacccgc tcaaccagcc
     2941 ggcgcggagc cagtttctgg cgatgatgaa ctgcccgtcc acaccagacc ggcagcggat
     3001 cgacaatcac acgcaggacg gcttcacgta cagcgtcaag ccgggggact atggcgtgat
     3061 gagcaacgtc agcggcggca cgctctatcc gctgggactt gtcgataaag cgactgacga
     3121 aagcgaggcg ggcatcatgc agaccaatcg cctgcaaact ctggctgaca tgcgcgatgg
     3181 cacgtcgaag accatcatga tggtggagga cgccggtcgg caccagcggt tcgtcgccaa
     3241 gacccgcaga atcaacggcc gctactcagg ctcgagttgc ctggaccatg aaaacatcat
     3301 gaccttgcac ggctacgaca acacgggcac ttccacgccc gggccctgtc cgctgaattg
     3361 caccaacaac aacgagatct acggctttca tcccggtggg gcgcaggtcg tcatgggcga
     3421 cggcgcggtg cggatgctga ccgcgggcat gcccctgcgg ctggtggccc ggctggtgac
     3481 acgcggcgcg ggcgacaacg gcggaaacga gtgaattcct gggcctgctc cggccgcaat
     3541 catggaaatc gcgggagtca acttgtggcg cgcgcgccat tcaatcgggg cggggctgct
     3601 attgattggg ctgctgcacg tttcaggctg tggcagcggc ggcccgccgc gtaagacctg
     3661 ctttcccgtc cgtgggcagc tctttgttgc cgatcagccg gccgacggtg caatgatcta
     3721 cttccaaccc caggatggca gtccgccgga ggaatggacc agcggctacc cgcgagcgcg
     3781 ggtcgcggcc gacggctcgt ttgaagtgag cacctatgcg gatcgcgatg gtgctccggc
     3841 cggcgactac gtgctcctcg tgcagtggat ccccggagcc gccaatgacg agggcgagcc
     3901 ctccgatgac gaatcatccg gcgaccgcct cggcggccga ttcatggacc ctgcctcctc
     3961 gccgctgcgg gccatcgtgg ggccggagcc gacgacgctg gaacggatcg acgtgcgctg
     4021 acatcgtagc cgcactcgcc agagtgcggg cgaaaaggac agcacgcccg cgttctggcg
     4081 aacacggcta cgacgcgctg cgtggctaag cagactcgct cagcgcagcc aacaagcgct
     4141 ggcggatttc ggaaaggcgc ggctcgtcgg tgagtttcgc gccgctgacg gcatcggcga
     4201 cgtaaaacac gtccacgacc tggtccaggt gcgtgc
//
LOCUS       DASUGB010000205         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_717899, whole genome shotgun sequence.
ACCESSION   DASUGB010000205 DASUGB010000000
VERSION     DASUGB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717899"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1128)
                     /gene="aroF"
                     /locus_tag="VFV87_04045"
     CDS             complement(67..1128)
                     /gene="aroF"
                     /locus_tag="VFV87_04045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5102955.1"
                     /translation="MNAYLPRFTGLRELFSMIVVMQREATGPQIQHMIQRVEALGLKA
                     HVIQGTERTVIAAVGEKRDNAKEALASGPGVDDVVPILAPYKVASREVKKEPTQVCVR
                     DLKVGAGACGIIAGPCSVESEEQILKTARAVKAAGATALRGGAFKPRTSPYSFQGLKE
                     EGLKLLAEAREETGLAVVTEVMATEDVELVAEYADVLQVGARNMQNYRLLEAVGQAKK
                     PVLLKRGPSATIDELLLAAEYILNEGNPEVMLCERGIRTFESHTRFTLPLATVPYLHH
                     KTHLPIVVDPSHGTGHTYLVPDMAAASIAAGADGLILEVHPDPERALSDGYQSLNFPQ
                     FLETIARCRSVAAAVGKVL"
     gene            complement(1468..1674)
                     /locus_tag="VFV87_04050"
     CDS             complement(1468..1674)
                     /locus_tag="VFV87_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102956.1"
                     /translation="MSAPKPESKVEFGSEFAASPKVDVTMPEVDSIQVIEDHDTRGDE
                     RFPPVRRPPVFPQVVAPPPESSQS"
     gene            complement(1678..2103)
                     /locus_tag="VFV87_04055"
     CDS             complement(1678..2103)
                     /locus_tag="VFV87_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102957.1"
                     /translation="MAEEAKPSGLLPFVRHMIVCEHAEPSPSNPRRTNVYGIFSSIVV
                     HGDAGAFPRSVGFSVYLSLTECRGDGEGRVIVSDAESGDMCYAGAPHDIILSTNPLAI
                     HEVIIRIPLCELPHPGVYLIAFEFDGVVLSQESLVVVVR"
     gene            complement(2348..>3096)
                     /locus_tag="VFV87_04060"
     CDS             complement(2348..>3096)
                     /locus_tag="VFV87_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5102958.1"
                     /translation="PVLPKVPVPTGLNWDLWLGQTPMVDYISAPSGGKYPHSRTHYEF
                     RWWYEYSGGKMTDWGAHHVDIATWAIGMDNSGPTSVEGVMAEHPVPYKDGWPTLDNQY
                     NAATKFDVKCLYPNGVELHVLSDGENGILITGDKGELFVSRGDLRGSAVDGLKDQPLP
                     EGAITKLYKGKKPGNHMGNFFDCIKDRTQPISDVYTHHRAMTTCHLANIAIRLGRPVK
                     WNPETEQIEGDEQANSFCKRQQRKGYEIRV"
BASE COUNT          568 a          965 c          946 g          617 t
ORIGIN      
        1 cagcgatttg gcggcgaagt agctcgcagc ccttagtcgg gaccgaagtc gccgccagta
       61 acaaggtcaa agcaccttcc ccaccgcggc cgccaccgac ctacagcggg cgatggtttc
      121 gaggaattgc gggaagttga gcgactggta gccgtcggag agggcccgct cgggatcggg
      181 gtggacttcg agaatcagtc cgtcggcgcc ggcagcaatc gaagcggcgg ccatgtcggg
      241 cacgaggtac gtgtgccccg tgccgtgact ggggtcgacg acgatcggca ggtgcgtctt
      301 gtggtgcagg tacggcaccg tcgccagcgg cagcgtgaaa cgggtatggc tctcgaacgt
      361 gcggatcccc cgctcgcaga gcatcacctc cgggttgcct tcgttgagaa tgtactcagc
      421 cgccagcaat agctcgtcaa tggtcgcgct cggcccgcgc ttcagcagca cgggcttctt
      481 agcctggccg accgcttcca gcagacgata gttctgcatg ttccgcgcgc cgacctgcag
      541 cacgtcggcg tattccgcga ccagctccac gtcctcggtg gccatcactt ccgtgacgac
      601 cgccaggccc gtctcctccc gcgcctcggc cagcagtttc agcccttcct ccttcagccc
      661 ctggaagctg taggggctcg tccgcggctt gaaggcgccg ccgcggaggg cggtcgctcc
      721 ggccgctttc accgcgcggg ccgttttgag gatctgctcc tcgctttcga ccgagcaagg
      781 ccccgcgatg atgccgcagg cgcccgcgcc caccttgaga tcgcggacgc aaacctgcgt
      841 cggctctttc ttcacctcgc ggctggcgac tttgtagggg gcgagaatgg gcacgacgtc
      901 gtcgacgccc ggcccgctgg ccagcgcctc cttggcgttg tcgcgcttct cgccgaccgc
      961 ggcgatgacc gtgcgctcgg tcccttgaat gacatgggcc ttgaggccca gcgcctcgac
     1021 ccgctggatc atgtgctgaa tctgcgggcc ggtggcctcg cgctgcatga cgacaatcat
     1081 cgagaataac tcccgtaagc cggtgaagcg tggcaggtaa gcgttcatgg tttggaccgg
     1141 gcaagtgagt ggtgagttgt gagtggtgag taaaggtcgt ttgcggcgcg actcactact
     1201 caccactcac cactcactcc ccttccagaa ccgaaaaccg ccgccctgag ttaatcgtta
     1261 gcatcgggcc aacaaaaaac cccgagcgac cttccggtcc tcggggctgt tggatttctg
     1321 gtgtctaggg atggattacc ggcacacctc cagcggcccc tcggatggga gggtaaagct
     1381 aaaccgccaa aagtagccga tgatccgtcg catggttgtc attatgccta tcggccagcc
     1441 ggttgcaagt gccgcaatcc aaacgcgtca agactgggac gactctggcg gcggggcaac
     1501 gacttgcggg aacacgggcg gacggcgaac cggcggaaat cgctcgtcgc cgcgcgtgtc
     1561 gtgatcttca atcacttgaa tcgaatcgac ttcaggcatg gtaacgtcca ctttgggcga
     1621 agcggcaaac tcactgccga actcgacctt cgattcaggc ttgggagcac tcataggtca
     1681 cctgacaacg acaactagcg actcttgaga cagaacaaca ccgtcaaact cgaacgctat
     1741 caagtataca ccgggatgcg gcaattcgca gagtggaatt cggatgatga cctcgtgaat
     1801 tgccagcgga ttggtgctca agatgatgtc gtgcggtgct ccggcatagc acatgtcccc
     1861 ggattctgcg tcagagacaa tgacgcgacc ttcgccatct ccgcggcatt cggtcagcga
     1921 caagtagact gagaacccga cacttcgcgg aaatgcgccc gcatcgccat gcaccacaat
     1981 gctgctgaaa attccgtaga cattcgtgcg tcgaggattg gaaggcgatg gctccgcatg
     2041 ctcgcaaaca atcatatggc ggacgaaggg cagaaggccg cttggcttcg cttcttctgc
     2101 cattcgcgtt gtccctccga tagttgcgaa atcaagcgtc ttccgacgtt cgcttgtcgg
     2161 gcgggcgcat tatcgcgtcg aggaagaatc caaaaactac acctcctgca aggctgctaa
     2221 ccacaacgac ggactgaatc gttcctgtgg tgtctgagca cgcgggcaat aggaggcaca
     2281 tgcaaaggat tccgcatccg gcgccaacag ccaaccacgg cactcgcatg atgggactcc
     2341 tgggcgatta cactctaatc tcatacccct tccgctgctg gcgcttgcag aagctgttgg
     2401 cttgctcgtc cccttcgatc tgctcggtct cggggttcca cttaaccgga cggcccaggc
     2461 ggatggcgat gttcgccagg tggcacgtcg tcatcgcccg gtggtgggta tagacgtcgc
     2521 tgattggctg cgtgcggtcc ttgatgcagt cgaagaagtt gcccatgtgg ttgccgggct
     2581 tcttcccctt gtacagcttc gtgatcgcgc cctccggcag tggctgatcc ttgaggccgt
     2641 cgactgcgct gccgcggaga tcgccgcggc tgacgaacag ctcgcctttg tcgccggtga
     2701 tcaggatgcc gttctcgccg tcggacagaa cgtgcagttc gacaccgttc ggatacaggc
     2761 acttcacgtc gaacttcgtg gcggcattgt actggttgtc gagcgttggc cagccgtcct
     2821 tgtacggcac ggggtgctcg gccatcacgc cttcaacgct ggtcggcccg ctgttgtcca
     2881 tgccgatcgc ccaggtcgcg atatcgacgt gatgggcccc ccaatccgtc atcttgccgc
     2941 cgctgtattc gtaccaccag cggaactcgt agtgcgtgcg actgtgcggg tatttgccgc
     3001 cggacggcgc tgagatgtaa tcgaccatcg gcgtttggcc gagccacaga tcccagttga
     3061 gaccggtcgg cacgggcacc ttgggcagca cggggg
//
LOCUS       DASUGB010000206         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1968934, whole genome shotgun sequence.
ACCESSION   DASUGB010000206 DASUGB010000000
VERSION     DASUGB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968934"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFV87_04065"
     CDS             complement(<1..325)
                     /locus_tag="VFV87_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102959.1"
                     /translation="MALALALATAAAAQKVGYLFGWLIGASLSPLGATVAPLLFGLLA
                     VLGLGGAMWRTPEGHALRRLRSFRALPPDQQREMQAELERSGGYGRLVLVAGGLWIFC
                     VSCQIG"
     gene            complement(424..609)
                     /locus_tag="VFV87_04070"
     CDS             complement(424..609)
                     /locus_tag="VFV87_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102960.1"
                     /translation="MPNATRMLVVLLVLVGSCPGDSPRPARRDIQQPAPTGSPWVDLP
                     AGPDDVDSRVGPISSGK"
     gene            complement(888..961)
                     /locus_tag="VFV87_04075"
     tRNA            complement(888..961)
                     /locus_tag="VFV87_04075"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Ile,seq:cat)
     gene            1241..2182
                     /locus_tag="VFV87_04080"
     CDS             1241..2182
                     /locus_tag="VFV87_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102961.1"
                     /translation="MRQKDYLLAGKDPPPKDAPAPDEGIQVGELCDRFFQAKEIQRNV
                     GELSPLSWSDYKRTCDAIVKQFGKSKPVAKLGAADFERLRAAFGKTRGLVAVGNEIQR
                     THVVFKYGYDSGLIEQPVRYGQLFKRAGKKRMRILRAKQEAEHGKKLFAPEEIKRLRS
                     TLIYVDSIEHAGNLLRYLPDKVSLLSGEMHLDHLQRVARQQIERSRSRHQEMQASVVA
                     VATADGLRKADLDSVEVIIRADAGSGLPDLDPWALTKPQGSDQQLLIIDIDERSGPIL
                     RRRSSMRKVAYLDADWFPVGMPSAHGRVERFIVNRPR"
     gene            2184..2828
                     /locus_tag="VFV87_04085"
     CDS             2184..2828
                     /locus_tag="VFV87_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX5102962.1"
                     /translation="MYQLKDILKYCAVAERRSQVIRELQLDQLPWQSPARCPSFHELT
                     EPDYLHAIADYVRRVGGPGPGPDGVRLSECTKTELWKIARDLGHLLQEESFLPSAARR
                     IRHKKPNGGYRELSLRSASTRVVSTALHFGFAPHFETVYLPCSYGFRRHIGPWHMLRD
                     MVQAIEQGFNIVVQADVEKAFDNVRPDIVLRDLRRHPIEDRICAHKQHSRSNRL"
BASE COUNT          605 a          932 c          882 g          504 t
ORIGIN      
        1 agccgatctg gcaactgacg cagaagatcc agagcccgcc ggccaccagc acgaggcggc
       61 catagccgcc gctcctttcc agctccgcct gcatctcccg ctgttgatcc ggtggcaacg
      121 cgcggaacga gcggagccgc cgcagcgcat ggccctcggg cgttcgccac atcgcgccgc
      181 cgagccccag caccgccagc agcccgaaga ggagaggtgc cacggtcgcg cccaaaggcg
      241 agagcgaagc gccgatcagc cagccgaaga ggtagccgac cttttgcgcc gcggcggcgg
      301 tcgccagcgc cagcgccagc gccaaccagt ggcggcggca ccaagcgcag caccagaaag
      361 ccgtgcgggg agcggcagcg agccagcttg tcgctctctg ccagcaggat ggcgccggtt
      421 cactcatttg ccgctgctga ttggtccgac gcggctgtcc acgtcgtccg gtcccgccgg
      481 cagatcgacc cacggggagc ccgttggagc cggctgctgg atgtcacgcc gcgcggggcg
      541 cggcgaatcg cccggacaac tgccgaccaa taccagcagc accaccagca ttctagttgc
      601 gtttggcacg gcgtcatccc tcccggttgt gacgtacgct ttcaatgggc gcttcgcccc
      661 accagcttgc ctatcctgcc cggcatgccg cgtgcgccgg aggactgaga atcctctggc
      721 tgccagagga ctgaggccgg cgagcgcgcc gcccagacgc tgctgactca aaactcggta
      781 gaaccggttt cagtggcggg gctttccccc gccccgtcca ggcggcgcgc ccgccggccg
      841 cagacgtggg actgcggcga cgagcaaaat ccaaactgaa tgttcagtag ggccgcccgg
      901 actcgaaccg ggaaccaaag gattatgagt cctctgctct gaccaattga gctacagccc
      961 caaacaccta ttgttactga atggattgcg tgtctgccgg ttgatcactg tggtccttga
     1021 caaccattcc gacaaccgcg taggctgcaa acggcccggc tgtctgtccc tactccgtaa
     1081 ggacgggtga gactatgagc aattctagca actcccgtaa gccgagtaag ccgcggaagg
     1141 actcccgctc actccacgga aagatggtcg ctggtgtaag aaagttcgcg gccgactgta
     1201 cctgtttgcc ggcacagcag acgaggccct ggcagaatgg gtgcgtcaga aagactactt
     1261 actagccggc aaagacccgc cccccaaaga cgctcccgcc cctgacgaag ggatacaagt
     1321 cggtgagctc tgcgatcggt ttttccaagc caaggaaatt cagcggaacg tcggcgagct
     1381 gtcgcccctt agttggagtg actacaagcg cacttgcgat gcgatcgtca agcagtttgg
     1441 gaagtcgaag cccgtcgcca agctcggggc agcagatttt gagcggttgc gggccgcgtt
     1501 cgggaaaacc cgcgggctgg tggctgtcgg aaacgaaatt cagcgcactc acgtcgtctt
     1561 caagtacggc tacgactcag ggctgatcga acaaccggtt cgctatggcc agctcttcaa
     1621 gcgggcagga aaaaaacgca tgcggattct ccgcgcgaag caagaggccg aacatgggaa
     1681 gaagctgttt gcgccggagg aaataaagcg gctgcggtca acgctgatct acgtcgattc
     1741 gatcgagcac gccggcaatc tactgcgcta tctaccggac aaagtcagct tgctcagcgg
     1801 agaaatgcac ctcgaccatt tgcagcgagt cgcgcgccag caaatcgaac gtagtcgcag
     1861 ccggcaccag gagatgcagg ccagtgttgt cgccgttgcg acagcagacg ggctgaggaa
     1921 ggctgaccta gactccgtcg aggtcatcat ccgcgctgac gccggttccg ggctgccaga
     1981 tttggatccc tgggcgctaa ccaagccgca aggcagcgat cagcagctcc tgatcatcga
     2041 catcgatgag cgcagcggac cgattctgcg gcggagatcg agcatgagga aggtcgctta
     2101 cctggacgcc gactggtttc cggtcggcat gccgtcagca catggccgcg tcgagagatt
     2161 catcgtaaat cgtccgcgat aacatgtatc agctcaaaga tatcctcaag tactgcgccg
     2221 tcgccgagcg gcgaagccaa gtcatccggg agctgcagct cgatcagctg ccgtggcaat
     2281 cacctgcccg ctgcccgtcc tttcacgagc ttactgaacc cgactatctc catgcgatcg
     2341 cggactacgt ccgacgtgtc ggcgggccgg gcccgggacc ggatggcgtg cgactcagcg
     2401 aatgcaccaa gaccgaattg tggaaaatcg cccgcgacct cggtcacctg ctgcaggaag
     2461 aatcgtttct gccgtcggcg gcaaggagaa ttcgccacaa aaagcccaat ggcggttatc
     2521 gtgagctcag cctccggtcc gcgtccacgc gcgtcgtttc gactgcgcta cattttggat
     2581 ttgctccgca ttttgagacc gtctaccttc cctgctcgta cggcttccgg cgccacatcg
     2641 gaccttggca catgctccga gatatggtgc aagcgatcga gcaaggcttc aacattgtgg
     2701 tgcaggctga tgtcgagaag gcgttcgaca acgtgagacc cgacatcgtc ctgcgagacc
     2761 tccgccggca cccgatcgag gacaggatct gcgcacacaa acagcactcg agatcaaatc
     2821 ggctttgacg atcaattcct tgcgcgctac agttcaggcg acccgtgttg tgcgggtcat
     2881 ggaagtttct cgcgcggccg accccacacc caggaacacc tcg
//
LOCUS       DASUGB010000207         4652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1829969, whole genome shotgun sequence.
ACCESSION   DASUGB010000207 DASUGB010000000
VERSION     DASUGB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829969"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1117
                     /locus_tag="VFV87_04090"
     CDS             197..1117
                     /locus_tag="VFV87_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102963.1"
                     /translation="MPIKVTCQCGKSFAAKDELAGRAVKCPNCQQPLRIPSPQAAGAP
                     AAQNLAAPLPSAAPRQAAASPGFSSPGGASSLFDEAGLKAAPAGSSLCPGCTSPLPAN
                     AVVCIKCGYNLKLGRRMETVRMAQPGIGPGGHEATTADLMHKAAISIEEDREEERKKT
                     REGMPWWVYGIGLLFCMGFIIMMMVLPQRIALVTGGVLMYGIAAIVSLYAQIRILIIA
                     FTESVAQGLMVLLVPCYGLVYVFMHWDSCGGYFLMQVAANVVANLVGFTLEATLGGEE
                     EARGAPPPPAAVATADFDWQPPPLLRASLA"
     gene            1137..1940
                     /locus_tag="VFV87_04095"
     CDS             1137..1940
                     /locus_tag="VFV87_04095"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5102964.1"
                     /translation="MIPKLLVWMACIQSPLLAIALFYSVAAAHAADEPGQQVPSSAEL
                     KLAGDDGGERTVTLRYLLYLPANYSADEKAAFPLLLFLHGSGERGEDLQLVKKHGPPK
                     LVDQEAHQKTWPFITVSPQCPKETRWNPAELAKLVEHVANTHRIDRRRIYVTGLSMGG
                     SGTWSLLAERPGLFAAAMPICGRGDPAAAEKIAKTPVWIFVGRKDKQELVENCGELDQ
                     ALRAAGGDSKVTYYDDLPHDCWTVTYDNPAVWQWLLAHQLAESKQTSSR"
     gene            1966..2778
                     /locus_tag="VFV87_04100"
     CDS             1966..2778
                     /locus_tag="VFV87_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HEX5102965.1"
                     /translation="MSHADPQNPYQSPQAYPPAAASAMPPPAHKLEKLEYLRAYNYIF
                     ENPEWTTVLLWGALMALLPVVGPLVLLGYIFVVIDALLESKGTRYPVLDLNRFVDYLV
                     RGLWPFLASLVVAVLLIPIYFVAWLVIMVGMMIAHQAGGEDAAGVAMLILMPIMFLIE
                     IVLILGLNIITLPLMLRAGLAQDFAEAFKFDWIKDFVRKMWLDTLLAMLFMTVTSMAL
                     VLVGYLACCIGVFFVQPIVMLAYAHFLYQLYAIYLTRGGQPVFAKPKLPSHV"
     gene            2823..3611
                     /locus_tag="VFV87_04105"
     CDS             2823..3611
                     /locus_tag="VFV87_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5102966.1"
                     /translation="MRHLVSFAMVVGSFAAFSLATAEEKPVEEKWVSLFNGHDLTGWT
                     PKIRGYELGDNFGETFRVEDGVMKVGYDKYGQFDEKFGHIFYKDKFSHYRLRVEYRFV
                     GDQCSGGPGWAIRNSGIMFHCQDPKTIGKDQRFPVSIEAQMLGGNGKDKRTTANVCSP
                     GTNIVMNDKLITQHCNSSKSETYHGDQWVTLEIEVHGSGKVLHKMNGQTVLEYEQPQL
                     DPKDADARPLIKDENNLLLEEGYISLQSESHPVEFRKVEILELR"
     gene            3696..>4652
                     /gene="dusB"
                     /locus_tag="VFV87_04110"
     CDS             3696..>4652
                     /gene="dusB"
                     /locus_tag="VFV87_04110"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX5102967.1"
                     /translation="MLLTEPLPEIKPLIIGPLKVDPPILQAPMAGFTNYAFRQIVRQY
                     GGAGLLATEMVSARGFVWMDANEAEHPDRLWGVKDEARPLAVQMWDNDPETLATVGER
                     LANEYRVSVVDINFGCPVKEVTEKAHSGSYLLKFPDRIHAIVSKVVAACRPTPVTAKI
                     RLGCSRDRITAFEVAQAVESAGAAALTVHGRVAADFFKGTADWERISEIKQHLQRIPL
                     IGNGDLDSAEKVVEAFRRYNVDGVMIARACLGRPWLFAQAAAALRGDPIPPEPTLAEQ
                     RACMLEHYELVCRRFGEEKGTLLMRKFGCNYAQGKPGARFFRT"
BASE COUNT          867 a         1469 c         1428 g          888 t
ORIGIN      
        1 tcgagtgtgg cgagtgttca ccactgtccg atcgcgcggc gcgggccgtt gactgcccgc
       61 cagattccaa ggggagaacg gcagctatcc gctgccggcc tatttcaccc gatgattgtt
      121 tcgccgcaaa ctccagcgca aaatacgttt cgactcgttc gctcgcgttt cgcaagtgtt
      181 gggggccggt tcgcccatgc cgatcaaagt cacctgccag tgcggtaagt cgtttgccgc
      241 caaggatgag ctggccgggc gggccgtcaa atgcccgaat tgccagcagc cgctccgcat
      301 tcccagccct caagcggccg gcgccccggc tgcccagaat ctggccgcgc cgctgcctag
      361 cgccgctccg cgccaggcgg ctgcatcgcc ggggttcagc tcccccggtg gcgccagtag
      421 cctgttcgac gaagccggcc tgaaagccgc tccggccggg tcgtcgctct gccccggctg
      481 cacgtctccc ttgccggcga atgccgtggt gtgcatcaag tgcggctaca acctgaagct
      541 cggccggcgg atggaaacgg tccgcatggc tcagccggga attggccccg gcggccacga
      601 ggcgacaacg gccgatttaa tgcacaaggc ggcgatttca atcgaagagg accgggaaga
      661 agaacggaag aaaacccgcg aagggatgcc ctggtgggtc tatgggattg gcttgctgtt
      721 ttgcatgggt ttcatcatca tgatgatggt cctgccccaa agaatcgcac tcgtaacggg
      781 cggcgttctc atgtatggaa ttgccgcaat tgtgagtctg tatgcgcaga tacgcatcct
      841 gatcattgcg ttcaccgaga gtgttgcaca aggtctgatg gtcttgttgg tgccctgcta
      901 cgggctcgtt tacgtcttca tgcattggga ctcctgcgga ggctatttct tgatgcaggt
      961 ggcggccaat gtcgtagcca atctcgtggg tttcacattg gaggccacgc tcgggggtga
     1021 agaggaggcc cgaggtgccc caccgcctcc cgctgcggtt gccaccgccg acttcgactg
     1081 gcagccgcca ccgctgctca gggcgagcct cgcctaatgc cttcgctgga cttccgatga
     1141 ttccaaagct gcttgtttgg atggcctgca tacaatcgcc gctgctggcg attgccttgt
     1201 tctactccgt cgccgccgcg cacgccgccg atgagcccgg ccagcaggtg ccgtcttcgg
     1261 ccgagctgaa gctggcgggg gacgacggcg gcgagcgtac cgtaacgctg cgttatttgc
     1321 tctatttgcc tgccaattat tctgccgatg agaaggcggc ctttcctctc ctcctcttcc
     1381 tgcacggcag cggggaacgc ggggaagacc tgcagctcgt gaaaaagcac ggcccgccaa
     1441 agctggtcga ccaggaggct caccagaaaa cctggccgtt tatcaccgtt tcgccgcagt
     1501 gcccgaaaga aactcgctgg aatccggccg aattggccaa gctggtcgag cacgtggcga
     1561 atacccaccg gatcgatcgc cggcggatct acgtcaccgg cctctcgatg ggcggcagcg
     1621 gcacgtggtc gctgctggcc gaaaggcccg gcctgttcgc tgccgcgatg cccatttgcg
     1681 gccgcggcga cccggccgca gcggagaaga ttgccaaaac gcccgtctgg attttcgtcg
     1741 gccgcaagga caagcaggaa ctcgtcgaga actgcggcga actggaccaa gccctaaggg
     1801 ccgccggcgg cgattccaag gtgacctatt acgacgacct gccgcacgac tgctggacgg
     1861 tgacctacga caacccggct gtctggcagt ggctcctcgc gcaccagctg gcggaatcga
     1921 aacagaccag ttcccgatga tccatctcgc gattcgagtt tcctgatgtc acacgccgac
     1981 ccgcagaatc cctaccaatc tccgcaagcg tatcctccag cggcagcgag cgccatgccg
     2041 ccgcccgcac acaaattgga gaagctggag tatctccggg cctacaacta catcttcgaa
     2101 aacccggaat ggacgacggt cctgctctgg ggcgcgctca tggcgctcct tccggtcgtg
     2161 gggccgcttg tcttgctggg atacatattc gtcgtgatcg acgcgcttct cgagtcgaag
     2221 ggcacgcgct atcccgtcct cgacctcaat cgatttgtcg attacctggt gcgcggccta
     2281 tggccttttt tggcgtcgct ggtcgtcgca gtcctgctga ttccgatcta ctttgtcgcg
     2341 tggctggtca tcatggtcgg aatgatgatc gcccatcaag ccgggggcga ggatgccgcc
     2401 ggcgtggcca tgctgattct catgccgatc atgttcctga tcgaaatcgt cctgatcctg
     2461 gggttgaaca tcatcacgct gccgctcatg cttcgcgccg gcctggccca ggactttgca
     2521 gaagccttca agttcgactg gatcaaagac ttcgtccgca aaatgtggct tgatacgctg
     2581 ctggccatgc tgttcatgac ggtcaccagc atggcgctgg tgctcgtggg ctacctggcg
     2641 tgctgcatcg gcgtgttctt tgtccagccg atcgtgatgc tggcttatgc ccatttcctg
     2701 taccagcttt acgccatcta cctcacccgc ggcggccagc cggtgtttgc caagcccaag
     2761 ctgcccagcc acgtctaacg gcgagcggta ggcgatgccg ttcattcgat cgaggatcaa
     2821 caatgcgcca tcttgtttct tttgccatgg tcgttggaag cttcgccgcc ttctcgctgg
     2881 cgacggccga agaaaaaccg gtcgaggaga agtgggtcag cctgttcaat ggccacgacc
     2941 tcaccggctg gacgccgaag attcgcggct atgagctggg cgataacttt ggcgagacgt
     3001 tccgcgtcga ggacggggtg atgaaggtcg gctacgacaa gtacggccag ttcgacgaaa
     3061 aattcggcca catcttctac aaggacaagt tctcgcacta ccgcctgcgg gtcgagtatc
     3121 gctttgtcgg cgaccagtgc agcggcggcc ccggctgggc gatccgcaac agtggcatca
     3181 tgttccactg ccaggacccg aagacgatcg gcaaagacca gcggttcccg gtctcgatcg
     3241 aggcccagat gctgggcggc aacggcaagg acaagcggac gaccgccaac gtctgctcgc
     3301 ccggcacgaa catcgtgatg aatgacaagc tgatcacgca gcactgcaat tcgtcgaagt
     3361 ccgagaccta tcacggcgac cagtgggtca cgctggagat cgaggtccac ggcagcggca
     3421 aggtgctgca caagatgaac ggccagaccg tcctcgaata cgagcagccg caactcgatc
     3481 ctaaagacgc cgacgccagg ccgctgatca aggacgaaaa caacctgctg ctcgaagagg
     3541 gctacatttc gctgcagtcg gagagccatc cggttgaatt caggaaggtg gagattctgg
     3601 agctcaggta gggtgggcta tgcccaccaa gagcggccga tgtaggttga agctgtaccg
     3661 ccgccaagac atggtgggca cagcccaccc tacgcatgct cctcaccgaa ccgctgccgg
     3721 agattaagcc gctcattatc ggcccgctga aggtcgatcc gccgatcctc caggccccga
     3781 tggccggctt cacgaactat gccttccggc agatcgtgcg gcagtatggc ggcgcggggc
     3841 tcttggcgac cgagatggtc tctgcccgcg gtttcgtgtg gatggacgcc aatgaagccg
     3901 agcaccccga tcggttgtgg ggcgtgaagg atgaggcccg gccgctagcg gtgcagatgt
     3961 gggacaacga tccggagacg ctggccacag tcggcgagcg gctggctaac gagtatcgcg
     4021 tcagcgtcgt cgatatcaac ttcggctgcc cggtgaagga agtgaccgaa aaggcccaca
     4081 gcggctcgta cctgctgaag tttcccgacc ggattcacgc gattgtctcc aaagtcgtcg
     4141 ctgcctgccg gccgacgcca gtgaccgcca aaatacgcct cggatgttcg cgcgatcgga
     4201 tcacggcgtt cgaagtggcc caggccgtcg aatcggccgg cgcggccgct ctcaccgttc
     4261 acgggcgcgt cgccgccgac ttcttcaaag gcacagccga ttgggagcgg atcagcgaga
     4321 tcaagcagca cctgcagcgc atcccgctga tcggcaacgg cgacctcgac tcggccgaga
     4381 aggtcgtcga agccttccgc cgctataacg tcgatggcgt gatgatcgcc cgcgcctgcc
     4441 tcggccgccc ctggctgttc gcccaggccg ccgccgccct ccgcggcgac ccgatcccgc
     4501 cggaaccgac gctcgcagag caaagagcct gcatgctcga gcactacgag ctcgtctgcc
     4561 gccgcttcgg cgaagaaaag gggacgctgc tgatgcgcaa gttcggttgc aactacgccc
     4621 agggcaaacc tggcgcgcga ttcttccgca cg
//
LOCUS       DASUGB010000208         6277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_857594, whole genome shotgun sequence.
ACCESSION   DASUGB010000208 DASUGB010000000
VERSION     DASUGB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857594"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..331)
                     /gene="rpsU"
                     /locus_tag="VFV87_04115"
     CDS             complement(119..331)
                     /gene="rpsU"
                     /locus_tag="VFV87_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5102968.1"
                     /translation="MVKLIVRDKESIQEAVRRFRKLVERSGIKKEMRRREYYEKPSEI
                     RRRARLRAERRARRTTMPAVGTRPGM"
     gene            650..3031
                     /locus_tag="VFV87_04120"
     CDS             650..3031
                     /locus_tag="VFV87_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102969.1"
                     /translation="MKRPHWRALLAGCLLVAGWLSAGFDPPRVRGQDQPSLHERIDQI
                     IESAAVGPIAPTCSDADFVRRIYLDLTGVIPTTEEMRAFLADESLDKRARLIEALLAS
                     PAFSRHMTLTLDVMLIERQPDKTNLLKPWLAYLLTSISANKPLDQLYREMLAADGADE
                     ALRPAARFTLGRDAEPNQMTRDIGRLAFGMDLQCAQCHDHPLIDDYYQADYYGLFAFV
                     QRTSLFNDAKKKQMLLAEKADGEANYKSVFTGDAADGVRPQLPKGAVLYVEPTFAKGE
                     EYVSAPAKDVRAVPKFSRRQALADMIPESNEFRRNVANRLWALMFGRGIVHPVDFHHV
                     DNPPAHPELLTLLAAELQAGGFNLRTMLRELALTRAYQRSCDAPQPEQVDFAHVAERL
                     AQLEGAKAERTAAVEPLKETLVKAKADRKAALDQNAGLAAQLKPLEMAVATAKTALDK
                     AAADRKAAEDDLAKKRDQAQAVADAAAKAAEAVAKIPDDKILAEAAAKIAERAKELAT
                     VIGGAEKLVSERTAQFDTATAAHAAAEGELGKVAASRVPPEKLVELERLELDTAHQVA
                     DTQYGVAALDAQIATAKHVAEYAELAKSDPVKAEAAWSALVERWTIANQVAALRPLTP
                     EQLAASAMRATGVLAAQEASAIAAIDKQPPDVLKNAADADKPRIRAEQIELRLIDQLD
                     NTFAEFAKLYGGQSGQDFQATVNQSLFFGNGGTIDGWLKTGGESLPVRLGKLEDPAAL
                     ADELYESVFSRPPADVEKQDVTDFLASRTDDKPIAISEMIWALLSSNEFRFNH"
     gene            3133..4422
                     /locus_tag="VFV87_04125"
     CDS             3133..4422
                     /locus_tag="VFV87_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102970.1"
                     /translation="MQCRYSCQSADHVASRRSFLGGLVAGTAGVATGLGGTLASTPAV
                     ADQIKSKSKRVLNIFLHGGVSQLETWDPKPNTDTGGPFRAIPTSVPGVHICELLPHTA
                     QQMHRLAIIRGVNTHNGDHGKGQEEMTTGRNRIPGADYPHLGAVAAKSLARDGFSLPG
                     HILVRGAGSGFSQSAYLGPKFASVVMDDGKPPKYTERPAALTAEIDERRNAFRAKVND
                     RFAGRRRTADTDAYTFSYQQAQDLLAQREVFDVTKEPAADHERYGQHEFGQHCLLARR
                     LLEHGVPFVQVNHSNYDTHFENFDFHIEQLGEFDKPFATLISDLAARGLLEDTLICVM
                     SEFGRTPKINANYGRDHWGTAWSVLVGGAGIQPGACVGKTNENGTKVVEREVDHGHIF
                     HTILRAVGVDSRAEFDIAGRKFPLADPAKGPIEELLV"
     gene            4529..5536
                     /locus_tag="VFV87_04130"
     CDS             4529..5536
                     /locus_tag="VFV87_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102971.1"
                     /translation="MSDAPTVDPTKAHQVFDFKHNRPLTACRWDPQGRYVFFGAEDNF
                     VHRLEIESKTLTSLPAHDSWVRALGCSPDGEFLYSGGYDGRLIWWPAAAEKPEPIRSI
                     DAHQGWLRALAVSPDGSKIATCGNDNFIKLWNAADGAPLAQLAGHETHVYNVAFRPDG
                     TALASCDLKGVVKEWDLATNTFKRDLTAAALWKYDTTFRADIGGARSIAFRGDGNQLA
                     LGGITNVSNAFAGIGHPAAVLLEWADGKVAFQLEAKEKANGVAWGLAHHPQGFWIMLT
                     GGGGGGWLIFFKGDSANEFHKLKLPNNGRDLSLSADGTRVAVAHADSQLRIYALHAKA
                     G"
     gene            complement(5678..6028)
                     /locus_tag="VFV87_04135"
     CDS             complement(5678..6028)
                     /locus_tag="VFV87_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5102972.1"
                     /translation="MPATAGVLLRDPLQIQPWAETCGQIRCLVEEQDGAAAEVHHVQI
                     DDAKLHYHERTDEFYYIIEGGGQMVLDDEKIDVRQGMVVYIPRGTRHKAIGKMTILTV
                     CIPPGVLGDIHEVE"
BASE COUNT         1180 a         2039 c         2000 g         1058 t
ORIGIN      
        1 aagcccccgc gaagagaaaa cggcgcggga agaaggcggg caatataccc accaggcaga
       61 caagaatgtc tgccccactt ggcccgcacc aggtcgagtg cggccacata gacaatcgct
      121 acatgccagg ccgggtgccg acggcgggca tggtggtgcg gcgggcgcgg cgttcagcac
      181 gcaggcgggc ccggcggcga atctcgctcg gcttctcgta atactcgcgg cggcgcattt
      241 ccttcttgat gccgctccgc tcaaccagct tgcggaagcg gcggacggct tcctggatgc
      301 tttccttgtc acggacaatc aacttgacca ctcgatcctc ctgtactggg gctgcttatc
      361 agtatcgacg ccaacggctc gcagtttttg ggaagctttg aagtgtacta cgtatggagt
      421 atttgtgtcc ataggctgtc cgagccctca aatcccacca gtagcagtct gcgtttttcc
      481 gaaattctgt tgaccccagt cttttgagtt tctattctca tagacgcgtg ttgatcggtt
      541 ctcggatggg ccgattgccg cccccgcacc aaatactccc gcccttcagc tcatcgtcgg
      601 cacgccaacg gatggacgcg ccggcttttt cccgccagga gccagcccca tgaagcgacc
      661 gcattggcgt gcgctgctag ctggctgcct gttggtggcg gggtggctga gcgccggctt
      721 cgatccgccc cgcgttcgcg ggcaagatca gccgtcgctg cacgagcgga tcgaccagat
      781 catcgagtcg gcggcggtgg ggccgatcgc tccgacttgc agcgacgccg atttcgtccg
      841 ccgcatctat ttggatttga ccggcgtaat tcccacgacg gaggaaatgc gggcgtttct
      901 ggccgacgaa tcgctcgata agcgggccag attgatcgag gcgctgctcg ccagcccggc
      961 ctttagccgc cacatgacgc tcaccctgga cgtaatgctc atcgagcgcc agcccgacaa
     1021 aaccaacctg ctcaagccgt ggctggcgta tttgctcacg tcgatttccg ccaacaagcc
     1081 gctggaccag ctttatcgcg agatgctggc cgcggacggc gcggacgaag cgctgcggcc
     1141 ggccgcccgc ttcacgctgg gtcgcgacgc cgagccgaac cagatgaccc gcgacatcgg
     1201 ccgattggcg ttcggcatgg acctgcaatg cgcccagtgc cacgaccatc cgctgatcga
     1261 cgattattac caggccgatt actacggtct gttcgccttc gtccagcgga cgagcctgtt
     1321 caacgacgcg aagaaaaagc agatgctgct ggccgaaaag gccgacggcg aggcgaatta
     1381 caagtcggtc tttacgggcg atgcggctga cggcgtccgg ccgcagttgc ccaagggggc
     1441 ggtgctgtac gtcgagccga cttttgccaa aggtgaggag tacgtctccg cgccggcgaa
     1501 agacgttcgc gcggttccca aattcagccg tcgccaggcg ctggcggaca tgatcccgga
     1561 gagcaacgaa ttccgccgca acgtggccaa ccgcctgtgg gcgctgatgt ttggccgcgg
     1621 gatcgtgcat ccggtcgatt tccaccacgt cgataatccg ccggctcatc cggagttgct
     1681 cacgctcctg gccgcggaac tgcaggccgg cggcttcaac ttgcggacaa tgctgcggga
     1741 gttggcgctg acgcgggcct atcagcgatc gtgcgatgcg ccgcagccgg agcaggtcga
     1801 tttcgcgcac gtcgccgagc ggctggcgca gcttgaaggc gcgaaggccg aacgcacagc
     1861 cgccgtcgag ccgctgaagg aaacgctggt aaaggccaag gccgatcgga aagcggccct
     1921 ggatcagaac gcgggcctcg cggcacagct caagccgctg gaaatggctg ttgcgacggc
     1981 gaagaccgcg ctcgacaaag ccgctgcgga tcgcaaggcg gcggaagacg atctggcgaa
     2041 gaaacgcgac caggcccagg ccgtggccga tgccgccgcc aaagctgcag aggcggtggc
     2101 gaaaatcccg gatgacaaaa tcctggccga agcggcggcc aagatcgctg agcgggccaa
     2161 ggaactggcc accgtgattg gcggagcgga aaagctggtc tctgagcgga ccgcgcagtt
     2221 cgacaccgct acggcggccc acgccgcggc tgagggcgaa ttgggaaagg tcgcagccag
     2281 tcgcgtgccg ccagagaagc tcgttgagct cgaacggctg gaattggata cggcccatca
     2341 ggttgcggat acacagtacg gtgtcgcggc gctcgacgcc cagatcgcga ccgcgaagca
     2401 cgttgcggag tatgccgagc tggccaagtc cgatccggtc aaagccgaag cggcttggag
     2461 cgcgctcgtc gagcgttgga cgatcgccaa ccaggtcgcg gccctccggc cgctcacgcc
     2521 cgagcagctt gccgccagcg caatgcgggc cactggtgta ctcgccgctc aagaggccag
     2581 cgccatcgcc gcaatcgaca agcagccgcc ggacgtcttg aagaacgccg ccgacgccga
     2641 caagccgcgg atccgcgccg agcagatcga attgcgcctg atcgaccagc tcgacaacac
     2701 gttcgccgaa ttcgcgaagc tctatggcgg ccagtcgggg caggactttc aggcgaccgt
     2761 caaccagtcg cttttcttcg gcaacggcgg gacaatcgac ggctggctca aaacgggcgg
     2821 cgaaagcctg ccggtgcggc tgggcaagct ggaagacccg gccgccctgg ccgatgaact
     2881 gtatgagagc gtcttctcgc ggccgccggc ggatgtcgaa aagcaggacg tgaccgattt
     2941 cctcgccagc cgcacggacg acaagcccat cgcgatcagc gaaatgatct gggccctgtt
     3001 atcgagcaac gagttccgct tcaatcactg acaaagccgc gaccattggt cgcggttccc
     3061 acaaactccg cgatcactca tcgcgggcac caactcccgc gaccactggt cgcggctttg
     3121 caggagactc ccatgcaatg ccgttactcc tgtcaatcgg ctgatcacgt cgccagccgg
     3181 cggtcgtttt tgggcggcct cgtcgcgggc acggcgggcg tggcgacggg cctgggtggg
     3241 acgctcgcat cgacgccggc cgtcgccgac cagattaaat cgaagagcaa gcgggttctc
     3301 aatatcttcc tgcacggcgg cgtgagccag ctcgaaacgt gggatcccaa gccgaacacc
     3361 gacacgggcg gcccgttccg ggcgattccc acgtccgtcc ccggcgttca catctgcgag
     3421 cttttgcccc acaccgccca gcagatgcac cgcctggcga tcatccgcgg cgtgaacacg
     3481 cacaacggcg atcacggcaa aggccaggag gaaatgacga ccggccgcaa ccggattccg
     3541 ggcgcggact atccgcacct cggcgccgtc gccgcgaagt cgctggcccg cgacggtttc
     3601 agccttcccg ggcacattct cgtccgcggc gccggcagcg gcttcagcca gtcggcgtat
     3661 ctgggaccga aattcgccag cgtcgtcatg gacgatggca agccgccgaa atataccgag
     3721 cggcccgcgg cgctcacggc tgagatcgac gagcggcgca acgcgttccg ggcgaaggtc
     3781 aacgaccgct tcgccggccg ccgccgcacg gctgataccg atgcttacac gttctcgtac
     3841 cagcaggccc aggacctgct cgcgcaacgc gaagttttcg acgtgaccaa ggagccggcc
     3901 gccgaccacg agcggtacgg ccagcacgag ttcggccagc attgcctgct cgcccgtcgg
     3961 ctcctggagc acggcgtccc gttcgtgcag gtcaaccact cgaattacga cacgcacttc
     4021 gagaatttcg acttccacat cgagcagctc ggcgaattcg acaagccgtt cgcgacgctc
     4081 atcagcgacc tggcggcccg cggcctcctg gaagacacgc tgatctgcgt gatgagcgaa
     4141 ttcggccgca cgcccaagat caatgccaac tacggccgcg accactgggg caccgcctgg
     4201 agcgtgctcg tcggcggcgc cggcatccag ccgggggcct gcgtcggcaa gacgaacgag
     4261 aacgggacga aggtggtcga gcgcgaagtc gatcacgggc acattttcca cacgattctg
     4321 cgggcggtcg gcgtcgattc gagagccgaa ttcgacatcg ccggccgcaa gttccccctg
     4381 gccgacccgg cgaaggggcc aattgaagaa ttgctcgttt aaccgttctt agccgggacc
     4441 aaacctggtc ccggagctgc gggactcggt gtccactcgc agttctccgg gagcaagttt
     4501 gctcccggct ggtgcaaatg gtgatgttat gagtgacgct ccgacggtcg acccgaccaa
     4561 agcgcatcaa gtcttcgact tcaagcacaa ccgcccgctg acggcctgcc ggtgggatcc
     4621 gcaagggcgg tacgtcttct tcggcgcgga ggacaacttc gtccatcggc tggaaatcga
     4681 gagcaagacg ctcacgtcgc tgccggccca cgatagctgg gtccgcgccc tcggctgttc
     4741 gccggatggc gagttcctct acagcggcgg gtacgacggc aggctgatct ggtggccggc
     4801 cgcggcggaa aagcccgaac cgatccggtc gatcgacgcc catcagggct ggctgcgggc
     4861 gctggccgtc agccccgacg gatcgaagat cgccacctgc ggcaacgaca acttcatcaa
     4921 gctttggaat gccgccgacg gcgcgccgct cgcccagctc gccggccacg agacgcacgt
     4981 ctacaacgtc gccttccggc ccgacggcac ggcgctggct tcctgcgatt tgaagggcgt
     5041 cgtcaaagaa tgggacctcg cgaccaatac ttttaagcgc gacctgaccg cggcggcctt
     5101 gtggaaatac gacacgacgt tccgggccga catcggcggc gcgcggagca ttgccttccg
     5161 cggcgacggc aatcagctcg ccctgggggg catcacgaac gtcagcaacg ccttcgccgg
     5221 catcggccac ccggctgcgg tgctcctcga atgggccgac ggcaaggtcg ccttccagct
     5281 cgaagccaaa gaaaaggcca atggcgtcgc ctgggggctg gcccatcacc cgcaaggctt
     5341 ctggatcatg ctcaccggcg gcgggggggg cggctggttg atcttcttca aaggggactc
     5401 cgccaacgaa ttccacaagc tcaagctgcc gaacaacggc cgcgacctgt ccctctcagc
     5461 cgacggcacc cgcgtcgccg tcgcccacgc ggacagccag cttcgcatct atgcgttgca
     5521 cgccaaggcg gggtgacgcc aggcgaccga ccaagccgat ctgacaggcg attgcgtcgt
     5581 ttaacggcga gccgaaggcg aagccgttgg atgcccgact gggcgccttc ggctacccgt
     5641 taaacgaagt acccgtcaag tcacgctggt gggcccgcta ctccacctca tgaatatccc
     5701 ctagcacgcc cggcgggatg cagacggtga ggatcgtcat tttgccgatg gctttgtgac
     5761 gggtgccgcg ggggatatag acgaccatgc cttggcggac gtcgatcttc tcgtcgtcta
     5821 aaaccatttg cccgccgccc tcgatgatgt agtagaactc gtcggtccgc tcgtgatagt
     5881 gcagcttggc gtcgtcgatc tggacatgat gcacctcggc cgccgcgccg tcctgctctt
     5941 cgaccaggca gcggatttgg ccgcaggtct cggcccaggg ctggatttgc aacggatcgc
     6001 ggagcaggac gccggcggtg gcgggcatgg gagagttcct tacgtggaga aggggcctgg
     6061 cacgcattct agtgcgattc cattttccta gcagcggcac ggcagtgctc aaagtagttt
     6121 cgacactttg gttgctgaaa ctggtcggtt cccgaatctc gtcgaagagt cgcccgcaga
     6181 gttcccagag tttatcctcc gtgtggtccg cgccgtggcg gggacgatcc tactgcgtcg
     6241 gacgggaagc gagggtatca tgatttggca atgcaaa
//
LOCUS       DASUGB010000209         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1134608, whole genome shotgun sequence.
ACCESSION   DASUGB010000209 DASUGB010000000
VERSION     DASUGB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134608"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFV87_04140"
     CDS             complement(<1..616)
                     /locus_tag="VFV87_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5102973.1"
                     /translation="MNDHAGEKPDTLQGLISRLHEEVAQIALGGGHAAIERQHAKGRL
                     TARERIERLIDPGSPLLELGLWAANGMYADWGGAPGAGVVTAIGTIGGRRHMIVANDA
                     TVKAGAFFPMTAKKVLRAQRIAFTNRLPLAYLVDSAGIFLPLQEDTFPDEDDFGRIFR
                     NNAVISAAGIPQYAAIMGNCVAGGGYLPVLCDKLLMTEGSGLYLA"
     gene            902..1615
                     /locus_tag="VFV87_04145"
     CDS             902..1615
                     /locus_tag="VFV87_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102974.1"
                     /translation="MKATWILSGAVMLVAGLAASASAQSYGSGSSYGFPGYGYGAGYG
                     VYHHASTYEEGVLRGLADLQRSGGEANYWHSLAANNWQEAYAKYLQNREAKTETYFRM
                     QAINRAAREATRPQRFTTEQLAVLAAKQAPDRLNPAQYDRTLGRLNWPAALQSDQFAE
                     ERAVLDSAFAARTPSDGGVGSAFGSGVRQLTDSMQARLQSQIRFMHQMDYLAAKKFLS
                     GLAIEAQQPLVVEALAAAE"
     gene            1756..2373
                     /locus_tag="VFV87_04150"
     CDS             1756..2373
                     /locus_tag="VFV87_04150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5102975.1"
                     /translation="MPARRSNRTTLLALAFEGGLGLIALGVGWLAGHWPAIGMSASAD
                     GTSKQLAAAGWGLVATLPLLVMLVLLDRFPVGPMRRLRDIAHDLVVRMFCGASYAQLA
                     LISLAAGFGEELLFRGLIQDGLARLIGGDFGPWIALAAASAAFGVCHWLNTTYAFLAM
                     LAGAYFGALLMLTGSLWTPIVAHAAYDFIALVYLVRPNHLIRSSV"
     gene            2414..3133
                     /locus_tag="VFV87_04155"
     CDS             2414..3133
                     /locus_tag="VFV87_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102976.1"
                     /translation="MPHRKSSYFVSDLHLFSRRSLAPRHEAALHAAAERAETFVLGGD
                     IFDFRWSTYHTTEETSRHAVRWLDALVGSHPQCDFHFVQGNHDCNRRFVSALETYSAT
                     RPNLSVHPYYLRLGKNVFLHGDAADHPLMCPDRLRTRREHWGRDERRRGEIRHALYDF
                     AVQARLHRLAGKVAHPRRRVVHRILGYLHKIGHAHDSGVEHVYFGHTHDALDHYRYRG
                     LTFHNPGAPIAGLDFRIVETQ"
BASE COUNT          515 a         1068 c          974 g          579 t
ORIGIN      
        1 cggccagata cagcccgctc ccttcggtca tcagtagctt gtcgcagagc accggcaggt
       61 aaccaccgcc agcgacgcag ttgcccatga tcgccgcata ctgcggaatt ccggccgcgg
      121 atatcacggc gttgttgcgg aagatgcggc cgaagtcgtc ctcgtcggga aacgtgtcct
      181 cctgcagcgg caggaagatg ccggctgaat cgaccagata ggccagcggc aggcgattcg
      241 taaacgcgat ccgctgggcc cgcaggacct tcttggcggt catcggaaag aacgccccgg
      301 cttttacggt ggcatcgttg gccacgatca tgtgccgccg tccgccgatc gtgccgatgg
      361 ctgtgacgac gccggcgccc ggtgctccgc cccaatccgc atacatgccg ttcgccgccc
      421 acaagcccag ttccagcagc ggcgatcccg gatcgatcag ccgctcaatt cgttcgcgcg
      481 ccgtcagccg gcccttcgca tgctgccgct cgatcgcggc atggccgccg ccgagggcga
      541 tttgtgccac ctcctcgtgc agccgcgaaa tgaggccctg gagagtatcc ggcttttcgc
      601 ccgcgtggtc gttcatgaag tgagcctatc gctagttcgt gcatcagttc catctcaaaa
      661 tgggagttcc gtcgtctcag gctgagattc cgcggcgctt gccgctttcc gccagccgct
      721 tctcagccgg ggcccatcgc ccgtaaatgc tacgcaattc tacgcttccg gcgatggcgt
      781 cacagcggcg atagctccgg cacaaatctt gccaaagtgg cgttcggtaa caaatcccgc
      841 gtcagcacgc ttagcaagtc aggttgggtg acaacggatt ttttcgagca agggcgagaa
      901 aatgaaagcg acttggattc ttagcggcgc ggtgatgctg gtcgccggcc tggcggcttc
      961 cgcttcggca cagagctacg gcagcggcag ctcttacggg ttccccggct acggctatgg
     1021 ggctgggtat ggcgtgtatc accacgcttc gacgtacgaa gaaggcgtcc tccgcgggct
     1081 ggcggacctg cagcgatcgg gcggcgaggc caactactgg cattcgctgg ccgcgaacaa
     1141 ctggcaggag gcgtatgcca agtacctgca gaaccgggaa gccaagaccg agacctattt
     1201 ccggatgcag gcgatcaacc gggcggcccg cgaggcgact cgcccgcagc gattcacgac
     1261 cgagcagttg gcggtgctgg cggccaagca ggcccccgac cgcttgaatc cggcccagta
     1321 cgaccggacg ctcggccgcc tcaactggcc ggccgccctg cagagcgatc agttcgccga
     1381 ggagcgagcg gtcctggatt cggctttcgc ggcccgcact cccagcgacg gcggtgtcgg
     1441 ctcggccttc ggcagcggcg tgcggcaact gaccgacagc atgcaggccc gcctgcagag
     1501 ccagatccgc ttcatgcacc agatggacta cctggccgcg aagaagttcc tctccggcct
     1561 ggcgatcgaa gcccagcagc cgctggtggt ggaagccctg gctgcggcag agtaacgcag
     1621 tcgtttaacg attagccgaa ggcgtcggcg aatagcgaaa gaaacacccg ctcgaaggac
     1681 gcggtcaggc gcacgtggcc tgccgcgtcc ttctctgttt cttggcataa tcggggcatg
     1741 gatgacgctc ctcccttgcc cgcacgacgg tccaaccgca cgacgctcct cgctctggcc
     1801 tttgagggcg gtttgggcct aatcgccctg ggcgtcggct ggctggcggg acactggcca
     1861 gcgattggga tgagcgccag cgctgatggg accagcaagc agcttgccgc tgcgggctgg
     1921 gggctggtgg cgacgctgcc gcttttggtg atgctcgttc tgctcgaccg ctttccggtc
     1981 ggcccgatgc gacggctgcg ggatatcgcc cacgatctcg tagtccggat gttctgcggc
     2041 gcgtcgtacg cgcaactcgc cctgatctcg ctggccgcag gcttcggcga agaattactt
     2101 ttccgtgggc tcatccagga cggcctggcc cggctgattg gcggcgattt cggcccttgg
     2161 atcgccctcg cggccgcctc cgccgccttc ggcgtatgtc attggctcaa cacgacttac
     2221 gccttcctgg cgatgctcgc cggcgcctat ttcggtgcgc tgctgatgct caccggcagt
     2281 ctgtggacgc ccatcgtggc ccatgcggcg tatgatttta ttgccctggt ttacctcgtc
     2341 cggccgaacc atctgatacg atcttctgta taagaaacag gccccgcgcc ttcccgcccc
     2401 gcggtcccca tccatgccgc accgcaaaag cagctacttc gtctccgacc tgcacctgtt
     2461 ttcccgccgt tcgctggccc cgcgtcacga ggcggccctt cacgcggcag cggagcgggc
     2521 cgagacgttc gtcctcggcg gcgacatttt cgacttccgc tggtccacgt atcacacgac
     2581 cgaggaaact tcgcggcacg cggtccgctg gctcgatgcc ctcgtcggca gccatccgca
     2641 gtgcgacttt cacttcgtcc agggaaatca cgactgcaac cggcggttcg tctcggccct
     2701 ggagacctac agtgccacgc ggccaaacct gagcgtccat ccgtactatt tgcggctggg
     2761 gaagaacgtc tttttgcacg gcgacgctgc ggaccatccg ctgatgtgcc ccgaccgcct
     2821 gcgcacgagg cgcgagcact gggggcgcga cgagcggcgg cggggcgaga tccgccacgc
     2881 cctgtacgac ttcgccgtcc aggcccggct gcaccgcctg gcgggcaagg tcgcgcaccc
     2941 gcggcggcgc gtcgttcacc gcatcctggg ctatctccac aaaatcggcc acgcccacga
     3001 ttccggcgtc gagcacgtct atttcggcca cacgcacgac gccctcgacc attaccgcta
     3061 ccgcggcctc accttccaca accccggcgc cccgatcgcg ggactggact ttcggatcgt
     3121 ggaaacccag taatgt
//
LOCUS       DASUGB010000210         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_301096, whole genome shotgun sequence.
ACCESSION   DASUGB010000210 DASUGB010000000
VERSION     DASUGB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301096"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFV87_04160"
     CDS             complement(<1..1286)
                     /locus_tag="VFV87_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5102977.1"
                     /translation="MNLGCPSFRQHSRRNFLRIGGASLCGVGLLDLLRARAQAAGANA
                     QPADRFTAPKAKQMIVCWMAGGPPHTDMFDMKPDSPADYRGEFKPIASKLPGLDVCEL
                     LPKLGAVADKYTIIRSITTLNNPGDHARAPMYWLTGNPRLPSGTAEFPMYGSAVAKFK
                     PGPPELPTFAVLGKIDHHINNSIAGSFLGPAFNPFIFDPNEKKDDIAKMLSPQLELPA
                     FAKQAELLKALDNRLRRQDGVDPLIAGLDAYQQTAFDMLRSPKLREAVDLSKEDEKTI
                     ARYKDGHLDKYRYPSGDPLHFLMARRLIEAGVPIVHFSLGYWDWHGENFVAARQQFPM
                     FDSGLSALLTDLDERGLLDTTIVLALGEMGRKPKCGTDAKAGRDHWDYAQFVLAAGGG
                     FKRGCIVGATDKLGEQVTDNFYKVESFGRTLYHLLG"
     gene            complement(1320..1982)
                     /locus_tag="VFV87_04165"
     CDS             complement(1320..1982)
                     /locus_tag="VFV87_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5102978.1"
                     /translation="MSNIVDHIGQLAERLRTVATDAEVEFTSYPSGSAMLDVRRQGRL
                     YVMSFSPQQGFGVDEVRDGEGFQNTYQFAFSDFQPAAQKLWELATASAHCPNIQQLST
                     APVELSLIVVYADDIARSKRFYEALGLTFSLEDHGGPEHYACRIGSAILEIYPRKGTG
                     SQSPPFRLGFRVPSVDRSVEILAREGSVVVTPAQDSPWGRRAVVKDPDGHSVEINSQQ
                     QA"
     gene            complement(2323..>3434)
                     /locus_tag="VFV87_04170"
     CDS             complement(2323..>3434)
                     /locus_tag="VFV87_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5102979.1"
                     /translation="IRKAEEKADTQADVAELRTKLAVLEQTIAENEAVQKEIRDKEQR
                     GRILSDGVPKRILHAQILHRPREEKSKRFASVTSPLGTQSSEQLRLLGESQALEVADN
                     EDPRNKLAEWLRRPDNPFFARAIVNRVWAHYFGRGIVDPPDDLSPYNPPTHPELLEEL
                     CRGFIENRYDLKWLHRAILTSRTYQQTSQATAENEMDRANYAFFYPRRLPAEVLVDAL
                     DQATGTREEFDMQYYHWPKPLRTVEVPYLPRNDFVVFLLEQFGRPPRNSSAQCDCARQ
                     VDSSLLQVLAFANHPRVRQKIADPGGRVAQVVQQTEDPPERIRQLYLSTLARLPDDAE
                     LAACQQFISEAASPQEGLQGVLWSLLNTREFVLQH"
BASE COUNT          592 a         1084 c         1075 g          683 t
ORIGIN      
        1 atgccgagca ggtgatagag cgtgcggccg aagctctcga ccttgtagaa gttatcggtg
       61 acctgctcgc cgagcttgtc ggtcgcgcca acgatgcagc cgcgcttaaa cccgccgccg
      121 gccgccagca cgaactgggc atagtcccag tggtcgcggc cggctttcgc gtcggtgccg
      181 cacttgggct tgcggcccat ttcgccgagg gccaggacga tcgtcgtatc gagcagcccg
      241 cgctcgtcga gatcggtgag caaagccgac aggcccgaat cgaacatcgg aaactgctgc
      301 cgcgcggcga cgaagttctc gccgtgccag tcccagtagc cgaggctgaa atggacgatt
      361 ggcacgcccg cctcaatcag ccgccgggcc atgaggaagt gcagcggatc gcccgaggga
      421 tagcggtact tgtccaagtg gccatcctta tagcgggcga tcgtcttttc gtcctctttc
      481 gacagatcga cggcctcgcg cagcttgggc gagcggagca tgtcgaaagc ggtctgctgg
      541 taggcgtcca ggcccgcaat cagcggatcg acgccgtctt gccggcgcaa gcgattgtcg
      601 agcgccttca gcagttccgc ctgcttcgcg aacgccggca gctccaactg cggtgagagc
      661 attttggcga tgtcgtcctt cttctcgttc ggatcgaaga tgaacgggtt gaacgccggg
      721 ccgaggaagc tgccggcgat cgagttgttg atgtgatggt cgatcttgcc caggacggcg
      781 aacgtcggga gctccggcgg gccgggcttg aacttcgcga ccgcgctgcc gtacatcggg
      841 aattcggcgg tcccgctcgg caggcgcggg ttgccggtca gccagtacat cggcgcccgg
      901 gcgtgatcgc ccgggttgtt cagcgtggtg atcgagcgaa tgatcgtgta cttgtcggcc
      961 accgcgccga gcttcggcag cagctcgcac acgtcgagcc ccggcagctt gctggcgatc
     1021 ggcttgaatt cgccgcgata atcggccggc gaatcgggct tcatatcgaa catgtcggtg
     1081 tgcggcggcc cgcccgccat ccagcagacg atcatctgct tggccttggg cgctgtgaat
     1141 ctgtcggcgg gctgcgcgtt cgcgcctgcc gcctgggccc gcgcccgaag taaatcgagc
     1201 aacccgacgc cacagagcga agccccgccg atccgcagga aattccgccg cgaatgctgt
     1261 cgaaacgatg ggcagccgag gttcatggcg gaggtccttc tatctatagg attcgtgctt
     1321 taagcctgtt gctgcgagtt gatttcgacc gagtgcccat cgggatcctt gaccaccgca
     1381 cgccgacccc acggagaatc ttgcgctggc gtgaccacaa ccgatccttc gcgcgcaagg
     1441 atttcaacgc ttcgatcgac cgaaggaacg cggaagccca gccggaacgg aggagattgg
     1501 gatccggttc cctttcgtgg gtagatttcc aggatcgctg agccaatccg acacgcataa
     1561 tgctcaggcc cgccgtgatc ctcgagagag aacgtgagcc ccagcgcctc gtaaaagcgc
     1621 ttggacctgg ctatatcgtc ggcatagacg acgatcaaac tcagctccac gggtgccgtg
     1681 ctcagctgct gaatgtttgg acaatgcgca ctagcggtag cgagttccca cagcttttgg
     1741 gcggcgggct gaaaatcgct gaatgcgaat tggtaggtgt tctgaaatcc ttcgccgtct
     1801 cggacttcat ccacgccaaa gccctgttgt ggcgaaaacg acatgacgta cagcctgccc
     1861 tgtcggcgca catccagcat ggcgctgccg gatggatacg acgtgaattc gacctccgca
     1921 tccgttgcca ctgtgcgcag acgctcggca agctggccaa tgtggtccac aatgttgctc
     1981 atggatctgt tgctccccgc ttctcgaatt ctatcgcgcc ggtcctcttg ttttgaatgg
     2041 tgatgctgcc gctcggtccg aggcgaatga cgtgtccgcg cgtcctcgct tccagtccgc
     2101 catccgctgt tcgtgcagta aagtcattct gcggcggata cattctaccg tcgggagcgt
     2161 ttggcggcgg agcaggcgac tgttttgcga ttccactcaa ctcgtcctct acttcctcca
     2221 gagttcgtgt aacttgctcc agggcttcgt cagcggtcgc cgcggctggc aacgtgccca
     2281 aacgacggta gtattcggca agccgttctc cgatcgtcat tgctagtgct gcagtacaaa
     2341 ctctcttgta ttcagcaagc tccacagcac gccctgcagg ccctcctgcg gcgacgcggc
     2401 ttcgctgatg aattgctggc aggcagccag ctcggcgtcg tccggcagcc gggccagcgt
     2461 gctcagatag agctgccgaa ttcgctccgg cggatcttcg gtctgctgga cgacctgggc
     2521 aacgcgtcca cccggatcgg caatcttctg gcgcacgcgg ggatggttgg cgaaggccag
     2581 cacctgcaag agcgacgaat cgacctggcg ggcacaatcg cactgcgccg acgaattccg
     2641 cggcggccgg ccgaactgct ccagcaggaa gaccacgaag tcgttccgcg gcaggtacgg
     2701 cacctccacg gtccgcagcg gcttcggcca gtggtaatac tgcatgtcga attcttcccg
     2761 cgtgccggtc gcctggtcga gggcgtccac cagcacttcg gccggcaaac gccgcggata
     2821 gaagaacgcg taattcgccc ggtccatctc gttttccgcg gtggcctggc tcgtctgctg
     2881 atacgtgcgg ctggtcagaa tcgcccggtg cagccacttc aggtcatatc ggttttcgat
     2941 aaagccgcgg cacagctcct ccagcagctc gggatgcgtt ggcgggttgt acggcgagag
     3001 gtcgtcgggc ggatcgacga tcccgcggcc gaaatagtga gcccagacgc ggttgacgat
     3061 cgcccgggca aagaacggat tgtccggccg gcggagccac tcggccagct tgttgcgggg
     3121 gtcttcgttg tcggccactt ccagcgcctg cgactcgccc agcaatcgga gctgctccga
     3181 cgactgcgtt ccgagcgggc tcgtcacgct ggcaaagcgc ttcgacttct cctcgcgcgg
     3241 gcgatgcaga atctgcgcgt gcagaatccg cttcggcacg ccgtcagaga gaatccggcc
     3301 gcgctgctcc ttatcgcgga tttctttctg cacggcctcg ttctcggcaa tcgtctgctc
     3361 caggacggcg agcttcgtcc gcagctccgc gacatcagcc tgcgtgtccg ccttctcctc
     3421 ggccttgcgg atgt
//
LOCUS       DASUGB010000211         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1830510, whole genome shotgun sequence.
ACCESSION   DASUGB010000211 DASUGB010000000
VERSION     DASUGB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830510"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFV87_04175"
     CDS             <1..825
                     /locus_tag="VFV87_04175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5102980.1"
                     /translation="AYANAILYKPPGQDVLWCFGMRCPMNSKNSEDAHLVGAFSADGG
                     RSWSPVELAMGYTGPLIVVAGIHRVLENGQPRYLLPAHRNTRRNDPLGTRDQFVLSST
                     SLLEWNLAAHIPQPASGKVFLHEGNIAPGEAEGELMMVMRTAQYEDERLATDPPRAYS
                     SISKDGGRTWSPAREEPELWNAKSKGFFSRAADGTHVYVYNDGPASARMSLRYKTKPA
                     GGNWSPEQTFYDAGIKNSYPTLIEVAPGDFRAVWDSGTKDRARTHIHFGKFRLDGK"
     gene            901..1779
                     /locus_tag="VFV87_04180"
     CDS             901..1779
                     /locus_tag="VFV87_04180"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX5102981.1"
                     /translation="MGTMLEPTTRPKIIQDAGEEPLVLYSDVRAPLARLSFLRRSLVL
                     AELAMVAYNDEAEAHWAAIEIGFPQATLIEQDGSQAYRFANRHDVVIACRGTEPTEWN
                     DIQADANATLAVVGTLGRVHSGFNREVDDVWPVLAPLLKRNRQPVWFCGHSLGAAMAT
                     ICAVRCRIEGIAANQQELHTFGSPRVGCRRWIRHASADHYRWVHNNDIVTRVPPPWMG
                     YRHCGREMYLDRFGRIRQLTGIWRSRDRWRGLLAGLLKWEIDWLADHSIKHYARHICA
                     AADQENSGTIQRRLAA"
     gene            complement(1783..2616)
                     /locus_tag="VFV87_04185"
     CDS             complement(1783..2616)
                     /locus_tag="VFV87_04185"
                     /inference="COORDINATES: protein motif:HMM:NF025619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEX5102982.1"
                     /translation="MPKDFDATTRHLLEVDPRAWLEYVGVPTTAPIEIINSDLSTITT
                     EADKVIRIADADPWLVHLELQSTYEQPLALRLARYNLLLEYRHRMPVLSVAVLLRQEA
                     DGRGLAGLLQATLPNGFCHYDFRYRVVRVWEQPVQQILAGGVATLPLAPLAAVSLNEL
                     PAVIRQIDERLTSEASVAEADAIWASTYILMGLRYPAEFAKQLLQQVRAMTESTTYQA
                     ILEEEAKRILMRQGHKRFGSPTPQIMAQIEAITDVDRIEQLAERLLDVNSWEELLVDS
                     R"
     gene            complement(2629..3828)
                     /locus_tag="VFV87_04190"
     CDS             complement(2629..3828)
                     /locus_tag="VFV87_04190"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX5102983.1"
                     /translation="MKYAIVIPDGCADEPQESLGGKTPLQAARTPAVDEIARLGVVGR
                     SNNVPAHLPPGSDVANLSLFGYDPHKYFTGRAPLEAAAQGIKLAPEDWAIRCNLVTIE
                     NQSMRSFTADHISTAEAAELLKTCQNELGTADLEFIPGVSYRNLLLYRGAKHAAPFTR
                     DTRATPPHDLTDKSVGDDFPRGPGSDFLTDLMGRSVPLFADHPVNVARKKAGKLPATN
                     IWLWGLGKTPALTPFKELHGKSGAMITAVDLLRGLAALIGWQRIDVTGATGYLDTDYA
                     AKGRAAIEALKSVDVVCVHVEAPDEASHEGRADAKIEALQQIDAKIVAPLHAALKAQG
                     DYRILVSPDHPTPIRTKTHSHGFVPFTICGTGVTRDAATTYDEVAAGQSKLAFEEGWK
                     LMGYFLR"
     gene            complement(3835..>4456)
                     /locus_tag="VFV87_04195"
     CDS             complement(3835..>4456)
                     /locus_tag="VFV87_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5102984.1"
                     /translation="IDALDPADLRYAKELGYRIKLIAVAQLHDGGLELHVSPTLVKIG
                     TPLAEVRQAFNAIQVIGDAVGPIFFHGLGAGQMPTASAVVADMIDTAVGRTGITFRTL
                     NLWSQGEAKVEMLDHKQLAGRYYLRFTVEDRPGVLAEIAGVLGKHQISIASVIQHEPS
                     GDGQQRTVPLVIMTYQATEGAAQGALAEIDRLKCVKTGSVRMRVLD"
BASE COUNT          778 a         1402 c         1464 g          812 t
ORIGIN      
        1 gcttatgcca acgcgatcct ctacaaaccg ccggggcagg acgtgctgtg gtgttttggc
       61 atgcgttgcc cgatgaacag caagaacagc gaggacgccc atctggtcgg tgcgttcagc
      121 gccgatggcg gtcgctcgtg gtctccggtc gagctggcga tgggctacac cgggccgctg
      181 atcgtcgtgg ccggaattca ccgcgtgctc gagaacggcc agccgcggta tctgctcccc
      241 gcccatcgca acacgcgccg caacgacccg ctcggcacgc gcgatcagtt cgtcctcagc
      301 agcaccagcc tgctcgaatg gaacctggcc gcccacattc cgcagcccgc cagcggcaag
      361 gtcttcctgc acgaggggaa catcgctccc ggagaagccg aaggcgagct gatgatggtc
      421 atgcgcaccg cccagtacga ggacgaacgg ctggcgaccg atccgccgcg ggcctattcg
      481 agcatcagca aagacggcgg ccgcacctgg agcccggccc gcgaggagcc ggagctgtgg
      541 aacgccaagt cgaagggctt tttcagccgg gccgccgatg gaacgcacgt gtacgtctat
      601 aacgacggcc ctgcctcagc ccggatgtcg ctgcgctaca aaacgaagcc cgcgggtggc
      661 aattggagcc ccgagcagac gttctacgac gcgggaatca aaaactccta tcctacgctg
      721 atcgaggttg ctcccggcga tttccgcgcc gtctgggaca gcgggaccaa ggaccgcgcc
      781 cgcacgcaca tccacttcgg caagtttagg ctggacggaa agtgattggg agcgacgctc
      841 gctcttgctc gaaagggcgc tctccagtaa gacgccgacc agcgaaagga gtcgctggcg
      901 atgggcacga tgctggagcc gacgaccagg ccgaagatca tccaggacgc cggcgaagag
      961 ccgctcgtcc tgtattcgga cgtgcgggcc ccgctcgccc gcctgagctt tctgcggcgg
     1021 tcgctggtgc tggcggagct agcgatggtt gcctacaacg acgaggcgga agcccactgg
     1081 gccgccatcg agatcggctt tccgcaggcc acgctgatcg agcaagacgg gtcgcaggcc
     1141 taccgctttg ccaaccggca cgatgtggtc atcgcctgcc gcgggactga gccgaccgag
     1201 tggaacgaca tccaggccga tgccaacgcc acgctggcgg tcgtcggcac cttgggccgg
     1261 gtccacagcg gcttcaaccg cgaagtcgat gacgtgtggc ccgtgctcgc gcctctgctg
     1321 aagcgcaacc ggcagccggt gtggttttgc ggccattcgc tgggggcggc gatggcgacg
     1381 atctgcgccg tgcgctgccg catcgaagga atcgccgcga accagcagga gctgcacacg
     1441 ttcggctcgc cccgcgtcgg ctgtcggcgg tggattcgcc acgcgtcggc ggaccattac
     1501 cgctgggtcc acaacaacga catcgtgacg cgcgtgccgc ctccgtggat gggctaccgg
     1561 cactgcggcc gcgagatgta cctcgaccgg tttgggcgca ttcgtcaact cacgggcatt
     1621 tggcgcagcc gcgaccgctg gcgaggactc ttggccgggc tgctcaagtg ggaaatcgac
     1681 tggctggccg atcacagtat taagcactac gcccggcaca tttgtgcggc cgcggatcag
     1741 gagaattccg ggacgatcca gcggaggttg gccgcgtagc cgctaacgcg aatccaccag
     1801 gagctcctcc caactgttga catccagcag ccgctcggcc aattgctcga tgcggtccac
     1861 gtcggtgatg gcctcgattt gagccatgat ttgcggggtg ggcgagccga accgcttgtg
     1921 cccttgccgc atgagaatgc gcttcgcttc ttcttccagg atcgcttgat aagttgtcga
     1981 ttcagtcatt gctcgcacct gctgtaaaag ttgtttcgcg aactccgccg gataccgcag
     2041 tcccatcagg atgtaagtgg acgcccaaat ggcgtcggct tcggcaaccg aggcctcgct
     2101 cgtgagtcgt tcgtcaatct gccgaatcac cgccggcaat tcgttcagcg aaacggccgc
     2161 cagcggcgcc aacggcaacg tcgcgacgcc gcccgccagg atctgctgca ccggctgctc
     2221 ccagactcgg acaacccgat agcgaaagtc atagtggcag aatccattgg gcagcgtcgc
     2281 ttgcaggagt cctgccagcc cgcggccgtc cgcttcctgt cgcaagagaa ccgcgaccga
     2341 aagcactggc attcgatgcc gatattccag taggagatta taccgcgcca gccgcaaggc
     2401 caaaggctgt tcgtaggtcg attgcaattc gaggtgcacg agccaggggt cggcatccgc
     2461 gatgcggatc actttgtccg cttcggtcgt aattgtggac agatcggagt tgatgatttc
     2521 gatcggggcg gtcgtcggca cgccgacata ttcgagccat gcccgcgggt caacttcgag
     2581 caggtgtcgc gtagtggcgt caaagtcttt gggcacgcgg cgcctcgctc acctcaagaa
     2641 ataccccatc agcttccacc cttcctcgaa tgccagcttg gattgccccg cggcgacttc
     2701 gtcgtaggtc gtggcggcgt cacgggtgac gccggtgccg cagatggtga aggggacgaa
     2761 gccgtggctg tgggttttgg tgcggatggg ggtgggatga tcgggggaga cgagaatgcg
     2821 gtagtcaccc tgggctttca gggcggcgtg aaggggggcg acgatcttgg cgtcgatctg
     2881 ttggagagcc tcaatcttgg cgtcggcccg gccttcgtgc gaggcttcgt cgggcgcttc
     2941 aacgtggacg cacacgacat caaccgattt cagggcttca atcgctgccc ggcccttggc
     3001 ggcgtagtcg gtgtcgagat agccggtggc gccggtcaca tcgatccgct gccagccgat
     3061 gagggcggct agaccgcgga ggagatcgac ggcggtgatc attgcgccgg attttccgtg
     3121 caactctttg aacggcgtga gggccggcgt cttgccaagg ccccacagcc agatgttcgt
     3181 cgcggggagc ttgccggctt tcttgcgggc cacattgacc gggtgatcgg caaagagggg
     3241 gacgctccgg cccatcaggt cggtgaggaa atcgctcccg ggcccgcgcg gaaaatcgtc
     3301 gccaaccgat ttatcggtca ggtcgtgcgg cggcgtggcc cgcgtgtcgc gcgtgaacgg
     3361 agcggcgtgc ttcgcgccgc gatagaggag cagattgcgg tagctgacgc cggggatgaa
     3421 ctccaggtcg gccgtgccga gttcgttctg acacgtcttg agcagctcgg ctgcttccgc
     3481 cgttgagatg tgatcggcgg tgaacgaccg catcgactgg ttttcaatcg tcaccagatt
     3541 gcagcggatg gcccagtctt ccggcgcgag cttgattccc tgcgctgccg cttccagcgg
     3601 ggcgcggccc gtgaagtact tgtgcgggtc gtaaccaaac aggctcaaat tcgccacatc
     3661 cgaccccggc ggcagatggg ccggcacgtt gttgctgcgt ccgacgacgc ccagccgcgc
     3721 gatctcatcc accgccggcg tcctcgcggc ctgtaacggc gtcttgccgc ccaaagactc
     3781 ctgcggctcg tcggcgcatc catcgggaat gacgatcgcg tatttcataa agttctaatc
     3841 cagcacccgc attctaacgc ttcccgtttt gacgcacttc agccggtcaa tctcggccag
     3901 agcaccctga gccgcacctt cggtggcctg gtaggtcatg atcacgaggg gcacggtgcg
     3961 ctgctggccg tcgccgcttg gttcgtgctg gatgacgctg gcgatcgaga tctgatgttt
     4021 gccgaggacg cccgcgatct cggccagcac gcccggccgg tcttcgacgg tgaaccgcag
     4081 atagtagcgg ccggcgagct gcttgtggtc gagcatctcg accttggctt ccccctgcga
     4141 ccagagattg agtgtgcgaa acgtgatgcc ggtgcggccg acggcagtgt cgatcatgtc
     4201 ggcaacgact gcactggcgg tcggcatctg cccggcgccg agcccgtgga agaagatcgg
     4261 ccctacggcg tcgccgatca cctgaatcgc gttgaacgcc tgccgcactt cggccagcgg
     4321 cgtgccgatc ttgacgagcg tcggcgagac gtgcagctcc agtccgccgt catgcaactg
     4381 ggccacggcg atgagcttga tccggtagcc gagctctttc gcatagcgca gatcagccgg
     4441 gtcgagtgcg tcgatg
//
LOCUS       DASUGB010000212         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1691546, whole genome shotgun sequence.
ACCESSION   DASUGB010000212 DASUGB010000000
VERSION     DASUGB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691546"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFV87_04200"
     CDS             complement(<1..710)
                     /locus_tag="VFV87_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102985.1"
                     /translation="MFLQTLVEFLFRLTFGVAAAMGVTPSREVTSGFFRIHLWVLLGV
                     QTLAALAIYSARPNGALADSDSSHMTVWKGQLVVSCAGAFLSYCGASIWMYETKLLGK
                     IAVWIVATLALLGCTLPLTLLNDRNFAFQLADRITSGALLGLVATSMLLGHWYLNTPT
                     MKLAPLQRLIALLAVALVLRAIVCGVGLGLELSLPGAVPAGQRTTWLIFVSLRWLAGL
                     VSVAGLAWLTRQTLKIPNT"
     gene            complement(789..1481)
                     /gene="folE"
                     /locus_tag="VFV87_04205"
     CDS             complement(789..1481)
                     /gene="folE"
                     /locus_tag="VFV87_04205"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5102986.1"
                     /translation="MRTSQMAEQLTKQRTTDAEDDSCCTGNGAVRAVGDGALASSVDL
                     PRIERAVREILAAVGENPDREGLLETPARVARMYAEMFSGLSQEPRQHTQKFFTATYD
                     EIVLVRDISFCSMCEHHLLPFIGHVHVAYIPEGKVIGLSKIARVVEVIARRPQIQEQM
                     TEQIANLLVDELGAKGVAVVIEASHSCMTIRGVKKPGSSMVTSAMKGLFRSNLSTRSE
                     VMQLIYGSRRET"
     gene            complement(1550..2386)
                     /locus_tag="VFV87_04210"
     CDS             complement(1550..2386)
                     /locus_tag="VFV87_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX5102987.1"
                     /translation="MRRHGFLIDMDGVIYRGKQLVPGAERFIARLLDEGVPFAFLTNN
                     SQRTRRDVATKLGRMGIEVQERHIFTCAMATARFLAAQKPGGTAFVIGEGGLLQALHK
                     NGYAIVDDEPDYVVVGEGRTFNMEIVEAAVRMILRGSRLIATNPDPNCPTSQGLRPGC
                     GAIVAMLEAATGLKAFSVGKPSPVMMRAARKELGLATADTIMIGDTMETDILGGVSMG
                     YRTTLVLTGSTCREDLVRYAYRPDLVVDSIADLCDKTVHATAVGELNGATVTFERHVL
                     AS"
     gene            2715..>3634
                     /locus_tag="VFV87_04215"
     CDS             2715..>3634
                     /locus_tag="VFV87_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102988.1"
                     /translation="MAATRLLFAAPFLLTVLAFALARADEEAPKLPPPASRTVEFAQD
                     IQPLLQKNCYSCHGPEMQEGGLRLDQKKRALEGGDSGAEIVPGKSAESRLVRMIAGID
                     EDFGRMPPEDKGTPLTAEQIRLVRAWIDQGAIWPEELAAADSSASYWSLQPVVAPPLP
                     EVADQAWLREPIDAFVLARLESKSIAHSPPAEKATLLRRLYLDLIGLPPSPEEVVAFL
                     ADESPDATERLVDRLLASPNFGERFARHWLDLARYADSDGYEKDRPRPFAWKYRDWVI
                     SAINADLPYDQFTLEQLAGDMLPGATDADRT"
BASE COUNT          700 a         1163 c         1127 g          644 t
ORIGIN      
        1 gtgttgggga ttttgagcgt ttgccgcgtg agccaggcca gtccggccac gctgaccaga
       61 cccgccagcc agcggaggga cacaaagatc agccaggtcg tccgctggcc cgcaggcacg
      121 gcacctggca gtgaaagctc cagccccagt cccacgccac agacaatagc ccgcaggacg
      181 agtgcaaccg cgagcagcgc aatcagccgc tggagcggcg cgagcttcat cgtcggcgtg
      241 ttcagatacc agtggccgag cagcatggaa gtcgcaacga ggccgagcaa ggcgccgctg
      301 gttatgcggt cggcaagctg aaatgcgaag ttcctatcgt tgagcaaggt caaaggaagc
      361 gtgcagccga gcagggcgag cgtagcgacg atccagaccg cgatcttgcc aagcagcttg
      421 gtctcataca tccagattga ggcaccgcaa tagctcagga aggcgccagc acaggacacc
      481 accaattgtc ctttccaaac ggtcatgtgg ctcgaatcgg agtcggcgag agcgccattt
      541 ggtcttgcgg aatagattgc tagcgccgcc agtgtctgca cgcccagcag cacccacagg
      601 tggatgcgaa agaatccgct ggtcacctcg cgcgacggcg tcacgcccat cgccgcggcc
      661 acgccgaacg tcagccggaa gaggaattcg acgagggtct ggaggaacaa ggcagtagga
      721 cgggtttgta acccgtcccc gcaatcggac gggttaaaaa cccgtcctac gaatgaagac
      781 gcccgtcgtc acgtttcccg ccgcgagcca taaatcaact gcatcacctc cgaccgcgtc
      841 gaaaggttcg agcggaacag ccccttcatg gcggaggtga ccatcgagct gccgggcttt
      901 ttgacgccgc ggatcgtcat gcaactgtgc gaggcttcga tcacaaccgc cacacccttg
      961 gcccccaact catcgaccag caaattcgcg atttgctccg tcatctgctc ctggatttgc
     1021 ggccggcggg caataacttc gacgactcgg gcaattttgc tgaggccgat caccttcccc
     1081 tcgggaatgt acgccacgtg gacgtgcccg ataaacggga gcagatgatg ctcgcacatc
     1141 gagcagaagc tgatgtcgcg gaccagcacg atctcgtcat aggtcgccgt aaaaaacttc
     1201 tgcgtgtgct gccgtggttc ctgcgacagg ccgctgaaca tttccgcata catccgggcg
     1261 acgcgggccg gtgtttccag caggccctca cggtcgggat tctcgcccac cgctgccagg
     1321 atttcccgaa cggcccgctc gatccgcggc agatcgacgc tggatgcgag cgcgccgtca
     1381 cctacggcgc gcaccgcgcc gttgccggtg cagcacgagt cgtcctcggc atccgtagtt
     1441 cgctgtttgg tcagttgctc ggccatctgg ctggttcgca caaagaggtg ctgccaacag
     1501 ccatcctaga tcgcagggcc caatcgggca acgacgtgcc cgccgcggat cagctcgcca
     1561 aaacgtgccg ctcgaaagtg acggtagcac cgttcagctc gcccacggcc gtcgcatgga
     1621 cagttttgtc gcacagatcc gcgatcgaat cgacgaccag atcgggccgg taggcataac
     1681 gcaccaggtc ctcgcggcag gtggagcctg tgaggacgag cgtggtgcga tagcccatgc
     1741 tcacgccgcc gaggatgtcg gtctccattg tgtcgccgat catgatcgtg tcggcagtgg
     1801 ccagccccag ttccttgcgg gcggcacgca tcatgacggg gctgggcttg ccaacgctga
     1861 acgccttcag gccggtcgcc gcttccagca tcgccacaat cgctccgcag ccgggacgga
     1921 gtccctggct ggtcgggcaa ttcggatcgg ggttggtcgc aatcagccgg ctgccgcgga
     1981 ggatcatccg tacggcggct tcgacgatct ccatgttgaa tgtccgcccc tcgccgacaa
     2041 cgacataatc cggctcgtca tcgacgattg catagccgtt cttgtgcagg gcctgcagca
     2101 ggcccccttc gccgattacg aacgcggtgc cgccgggctt ttgggcggcg agaaatcggg
     2161 cggtggccat cgcacaggtg aagatgtgcc gttcctgtac ctcaataccc atgcggccga
     2221 gcttcgtcgc cacatcgcgg cgcgtgcgct gactgttgtt agtcaggaat gcgaatggaa
     2281 cgccttcgtc gagtaagcgg gcgatgaacc gttcggcgcc gggcacaagc tgcttgcccc
     2341 ggtagatcac gccgtccatg tcgatcagaa atccatgcct cctcatcggc aatccctgag
     2401 aacgtggacc gcgccgcgaa gtctcgcccc aagcgaagtt acgcagtgcg gtggaccgcc
     2461 tgacggatgc cgccacaatg caaggggtgt gccgcagcgg gattgccgtg gaatcgctgg
     2521 ccgggctgcc agttcgccgg gcaaaggctg ccgcgcggat gtacagcggc agataattgc
     2581 cgtttatttc caaaagcttg tttgacgcac tccaagccta ttggtcaaaa tggagaaacc
     2641 taccggcgat gcctcgcggg cgagtgcctg cgccccgatg gccgccaaaa cgctgccgct
     2701 tttgcgatcc tttgatggct gcgacacgac tcctctttgc cgctcctttc ttgcttacgg
     2761 tgcttgcctt cgcgctggca cgcgcggacg aagaagcacc aaaactgccg ccccctgcga
     2821 gccgaaccgt cgagttcgcc caggacattc agccgctgtt gcaaaagaac tgctattcgt
     2881 gccatgggcc cgagatgcag gaagggggcc tgcggttgga tcagaaaaag cgggccttgg
     2941 aagggggcga cagcggggcg gagatcgtcc ccggcaagag cgccgagagc cggctggtgc
     3001 ggatgattgc cgggattgac gaagacttcg gccggatgcc gccggaagac aaaggaacgc
     3061 cgctgaccgc cgagcagatc aggctggttc gggcctggat cgatcaagga gcgatttggc
     3121 cggaggaact ggccgcagcc gattcgtccg cgagctattg gtcgctccag ccggtcgtcg
     3181 ctccgccttt gccggaagtt gctgaccaag cctggctccg cgaaccgatc gatgcgttcg
     3241 tccttgcccg cttggagagc aagagcatcg cccactcgcc gccagcggaa aaggcgaccc
     3301 ttctgcggcg cctctatctg gacctgatcg gcctgccccc ttcgccggag gaagtcgtcg
     3361 cctttctggc cgatgaatct cccgacgcca cggaacgcct tgtggatcgg ctgctcgcct
     3421 cacccaattt cggcgagcgc ttcgcccggc actggctcga cctggcccgc tatgccgata
     3481 gcgatggcta cgagaaggac cgcccgcggc cgttcgcgtg gaaatatcgc gactgggtca
     3541 tctccgcgat caacgccgat ctgccctacg accagttcac gctggagcag cttgcaggcg
     3601 acatgctgcc aggcgcgacc gatgccgacc gtac
//
LOCUS       DASUGB010000213         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1553648, whole genome shotgun sequence.
ACCESSION   DASUGB010000213 DASUGB010000000
VERSION     DASUGB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553648"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2832)
                     /locus_tag="VFV87_04220"
     CDS             complement(<1..2832)
                     /locus_tag="VFV87_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5102989.1"
                     /translation="MLARSFLAAGFLIAIAGTALAADDRVDFNREIRPLLAKKCFACH
                     GPDDEHREAGLRLDSQAGATKELDSGAVAVVASNSGKSELVRRVQSSDDSERMPPAET
                     GITLTAEQTDLLKRWIDQGASFAPHWSFVKPERPAISKLDIVEHAATPIDFFVVERLT
                     AAGLAPAPRADRYALIRRLSLDLRGLPPTPEEAAAFVADQSPDAIERLTDRYLADPAY
                     GERWARVWLDLARYADSRGYGSDPLRPNIWRYRDWVIDAFNANQPFDQFTIDQLAGDL
                     LPNATLEQKVATAFHRNTMTNTEGGTDDEEFRVAAVKDRADTTMQVWMGITLGCAKCH
                     NHKYDPISQHEYYSFYAIFNQTADSDKGDDSPFIQAPTPEYQTRVRLIDEQIAAVRQK
                     LETPTPEVAAEQAKWEAGLALKADWQPLAPVEMSSAGGATLTSQDDGSILVSGENPNQ
                     DTFTVAAQTDLTGMTALRLETIPDPTLPQGSAARSLSGDFRLTRFSATAQDPNSSDSA
                     ATGRYVRIELPGKGKMLSLAEVQVFDGADNIALKGTAKQSSTDFEGEAKRAIDGDTNG
                     NYYEANSTTHTRIEADPWWEVDLGGDKRIERITLWNRTDGGDAIGSRLAGYKVAILDA
                     ERKVVWQQAPADFPKPTTELAASGVTTIAFAEALADFSASGLPVASIIKTPADPKAGW
                     SVGDQRAQPHWAAFAFAAPLAQQGRALLKIKLEFQSDQPQQTLGRFRLSATSDAAILN
                     RLKVPAEVLAIVDTPAGSRTPDQLAKLAAHYRTLAPSLKPLRDQIAALEKSKPPMPTV
                     PVFQQLVADQQRKTFIQLKGNFLQKGDEVSASLPAAWHSLEEGAPRDRLGLARWIVHP
                     DNPLTARVMANRLWAQLFGAGIVETEEDFGIQGEQPSHPELLDWLAIEFQQAWDIKAL
                     LRTIVSSDTYRQSSHFTPESLEKDPRNRLL"
BASE COUNT          480 a          967 c          990 g          572 t
ORIGIN      
        1 aagcaggcgg ttccgcgggt ccttttcgag cgactcggga gtgaagtggg acgattggcg
       61 gtaggtgtcg gacgaaacga tcgtccgcag caaagccttg atgtcccaag cctgctggaa
      121 ctcgatcgcc agccaatcga gcagctccgg atggctcggc tgctcgccct gaatgccgaa
      181 gtcctcttcg gtttcgacga tccccgcccc gaaaagctgc gcccagaggc gattggccat
      241 gacgcgggcg gtgagcggat tgtctggatg cacgatccac cgcgccagac ccagccgatc
      301 gcggggcgcg ccttcttcga gcgaatgcca ggcagcgggc agagaagccg aaacttcgtc
      361 ccccttctgc aggaagttgc ctttgagctg aatgaatgtc ttccgctgct ggtcggccac
      421 aagctgctga aagaccggta ccgtcggcat cggcggcttc gatttttcca gcgcggcgat
      481 ctggtcacgt agcggcttga gcgacggtgc gagagtgcgg taatgggcgg ccagcttggc
      541 gagttgatcg ggtgtgcggc tgccggcggg cgtatcgacg atcgccagaa cctccgccgg
      601 caccttcagc cggttcagga tcgccgcgtc gctcgtcgcc gaaagtcgaa atcgcccgag
      661 cgtctgctgc ggctggtcgc tctgaaattc aagcttgatc ttcagcagcg cccggccctg
      721 ttgcgccagc ggcgcggcga aggcgaacgc cgcccagtgc ggctgagcgc gctgatcgcc
      781 cacgctccaa ccggctttgg gatcggcggg cgttttaata atgctggcga cgggcaggcc
      841 gctggccgag aagtcggcca gggcttccgc gaaggcgatg gtcgtcacgc cgctggcggc
      901 cagctctgtg gtcggctttg gaaaatcagc cggagcctgt tgccacacga ccttgcgctc
      961 ggcatcgaga atcgcgacct tatatcccgc cagccggctg ccgatcgcat cgccgccgtc
     1021 agtgcggttc cagagggtga tccgctcgat ccgcttgtct ccgcccagat cgacttccca
     1081 ccacggatcg gcttcgatgc gggtatgcgt cgtcgaattc gcctcgtagt agtttccatt
     1141 cgtgtcgccg tcgatcgccc gtttggcttc gccctcgaaa tcggtgctgg attgctttgc
     1201 cgtgcctttc agggcgatgt tgtccgcgcc gtcaaacacc tgcacctccg ccagcgagag
     1261 catcttgccc ttgccgggca gctcgatccg cacgtagcgg ccggtcgcgg ctgagtcgga
     1321 cgaattcggg tcctgggcgg tggccgagaa gcgcgtcagc cggaaatcgc cgctcagcga
     1381 acgggcggct gagccttgcg gcaacgtggg atcgggaatc gtctccagcc gcagcgccgt
     1441 catgcccgtg agatccgtct gcgcggcaac cgtgaacgtg tcctgattcg gattttcgcc
     1501 gctgacgaga atcgagccgt cgtcctgact ggtcagcgtg gcgccgccgg cggacgacat
     1561 ttcgaccggc gccagcggct gccagtccgc cttgagcgcg agtcctgcct cccacttcgc
     1621 ctgctcggca gccacctccg gcgtcggcgt ttccagcttc tgccgcacgg ccgcgatctg
     1681 ctcgtcgatc agccgcacgc gcgtctgata ttcgggcgtg ggggcctgaa tgaacggcga
     1741 atcgtcccct ttgtcgctgt ccgcggtctg attgaagatc gcatagaacg agtagtactc
     1801 gtgctgcgag atcggatcgt atttgtggtt gtggcatttc gcacagccga gcgtgatccc
     1861 catccagacc tgcatcgtcg tatcggcccg gtccttcacc gcggcaacgc ggaattcctc
     1921 gtcgtcggtg cccccttcgg tgttggtcat cgtattgcga tgaaaggccg tcgccacttt
     1981 ctgctcgagc gtggcattgg gcaacagatc gccggcaagc tgatcgatgg tgaactggtc
     2041 gaacggctgg ttggcgttga aggcgtcgat gacccagtcg cgataccgcc agatgttcgg
     2101 ccgcaaagga tcgctgccat agccccgcga atcggcatag cgggccaggt cgagccagac
     2161 ccgcgcccac cgctcgccat aggccgggtc ggccagatag cggtcggtca agcgctcgat
     2221 cgcgtcgggc gattgatccg caacaaacgc cgcggcttcc tccggcgtcg gcggcaggcc
     2281 gcggaggtcg aggctcaggc ggcggatcag tgcgtagcga tcggcccgtg gggcaggcgc
     2341 taggcctgcc gcggtcaggc gctccacgac gaagaagtca atcggcgtcg ctgcgtgctc
     2401 cacaatatcg agtttcgata tcgcgggccg ctccggcttg acgaacgacc agtgcggcgc
     2461 gaacgacgcg ccctgatcga tccagcgttt caagaggtcc gtttgctcgg cggtcagcgt
     2521 gatgccggtc tccgccggcg gcatccgctc gctgtcgtcg ctgctctgca cgcggcgaac
     2581 cagctcgctc ttgccgctgt tgctcgcgac caccgctacc gcgccgctgt ccagctcctt
     2641 cgtcgcaccg gcctgcgaat ccagccgcaa gcccgcctcg cgatgctcgt cgtccggtcc
     2701 gtggcaggca aagcacttct tggccagtag cggccggatt tcgcgattga aatcgacgcg
     2761 atcatcggcg gccagtgcgg ttccagcaat ggcgatcagg aatccggcgg cgagaaatga
     2821 tctggcgagc atcgcagtgg cgggaagggt gaggcggggc ggctcgaacc accccagatt
     2881 ataacacgac agccgcctcg tttaacgcgg agccgcaggc gacgcggctc gctgcgccag
     2941 cagggggtcg ggagtcaatt gcggttatcg tcgtgccatg tggtgggtcg tcggccgcaa
     3001 ttgactccc
//
LOCUS       DASUGB010000214         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1553669, whole genome shotgun sequence.
ACCESSION   DASUGB010000214 DASUGB010000000
VERSION     DASUGB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553669"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFV87_04225"
     CDS             complement(<1..691)
                     /locus_tag="VFV87_04225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5102990.1"
                     /translation="MDTSATIVVPCYNEAQRLDVARFRNFARQARGLVLRLVNDGSTD
                     GTLELLRELERDNPRAIVVQDLPRNGGKAEAVRQGILAALRQQPCPQYIGYWDADLAT
                     PLEAIEPFIRVLDSRPDLLFVLGARLPLLGRRIERTFKRRMCGRAFAFAASLALGISV
                     RDTQCGAKMFRVTPETRRLFDSPFSSRWIFDVEILARLLWQADRSERTHLTSTIYELP
                     LDRWAEIEGSKL"
     gene            complement(698..2794)
                     /locus_tag="VFV87_04230"
     CDS             complement(698..2794)
                     /locus_tag="VFV87_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102991.1"
                     /translation="MAALTISVLVVAIHLVLGLLAPGFLLESLCRPLRRPGEALSCLV
                     SRVLALGSLWLMVWFFAVNPAMLQWRVPPHWLTAALWLIGLAALVALVTWRRRDFSEV
                     ARQLAREAAQPLTLAVVLLAMGVAALGIFRCPYALDNAALGWTGRALDGSITSFGDSQ
                     GSPAYIALLYFPAAVTWPGIPVPTTGAALKIPLCLLAALAAMRLAASLPFRFPRAIGA
                     GLFLVLIGSFFGVYGMFETGKETPFGIALLMIFVGELLDADSREWAWAVRAGLVLCAA
                     FGFGAITIPYAIPLVGIFLAANIGRVRTFEFSFWLLACTALPLVFSIYAMTHVALWKA
                     AVLVAVLTTICAALSRLRWRLPAPPAHWVSAAAVIVALVCLAGIYFALPVRYTVPHPP
                     LDGQTGFFQLWADSDRVLHDAIIVAGFVGLLASCFLPGFAHRPGLIALALFPFATLLP
                     VLIIAQFPDWKPPFHPQHVWDLAKDVPNWCFGFYFGLFALIAVEAVGRVIEHVVQRRA
                     VAGGKLAARLPAWLPQAIVTAAGVLALATTLDRPHIRHGYHLKRPAHFTRVGGHQDPL
                     FARLVERFATEQKPPARPVRGNQKGRLFVSATSEVNSRRHDFGMYGINVRSDWSPGEP
                     QDLAALEQGRAILVAPPDEAAALEQAHERLHVELLEPLTLTACVYLITPDQQPTPTAG
                     QPRKSPFRTVSHSHHD"
BASE COUNT          622 a          940 c          998 g          382 t
ORIGIN      
        1 gaagcttgga gccttcgatc tccgcccagc ggtcgagcgg cagctcgtag atggtgctcg
       61 tcaaatgggt gcgctcggag cgatccgctt gccagagcaa tcgggcgaga atctcgacgt
      121 cgaaaatcca ccgcgaggag aaaggcgaat cgaacaggcg acgcgtctct ggcgttacgc
      181 ggaacatctt cgcgccgcac tgcgtgtcgc ggaccgaaat tcccagggcc aacgacgcgg
      241 cgaaggcgaa cgcacggccg cacatccggc gcttgaacgt ccgctcgatg cggcggccga
      301 ggagcggcaa gcgggcgcca agaacaaaca gcaggtctgg acgcgaatcc agcacgcgga
      361 tgaacggctc gatcgcttcc agcggcgtcg ccaggtcggc gtcccagtag ccgatgtact
      421 gcgggcacgg ctgctggcgg agggcggcca ggatgccttg ccgcaccgct tcggccttgc
      481 cgccgttgcg gggcaggtcc tgcacgacga tcgcccgcgg attgtcccgc tccaattcgc
      541 ggagcaattc cagcgtgccg tcggtgctgc cgtcgttcac cagccgcagc accaggccgc
      601 gcgcctggcg ggcgaaattg cgaaagcgag cgacgtcgag gcgctgggct tcgttgtaac
      661 acgggacaac gatagtggcg gaagtgtcca tgcgagttca atcgtgatgg gaatgtgaaa
      721 cggtacgaaa gggcgatttt cgtggctggc ctgcagtcgg cgtcggctgc tggtcgggag
      781 taatgaggta cacgcacgcc gtcagcgtca gcggctccag caattcgaca tgcaatcgct
      841 cgtgggcttg ctccagcgct gccgcttcgt ccggcggagc gacgaggatg gcccgtcctt
      901 gctcgagtgc cgcgaggtct tggggctcgc caggcgacca atcgctgcgg acgttgatgc
      961 catacatgcc gaaatcgtgc cggcgcgagt tgacttccga ggtcgcactc acgaagaggc
     1021 ggcccttctg gttgccgcga accgggcggg ccggcggctt ctgttccgtg gcgaaacgct
     1081 cgacgaggcg ggcgaacagc ggatcctgat gcccgccaac gcgggtgaag tgtgcgggcc
     1141 gcttcagatg gtagccgtga cggatgtgtg gccggtccaa cgttgtcgcc agggccagaa
     1201 cgccggcggc cgtgacgatg gcctgcggca gccaggccgg caggcgcgcg gcgagctttc
     1261 cgcccgctac ggctcggcgc tggacgacgt gctcgatgac ccggccgacc gcttcgaccg
     1321 cgatcaaggc gaacaggccg aagtaaaacc caaagcacca gttgggaacg tccttggcca
     1381 ggtcccacac gtgctgcgga tggaacgggg gcttccagtc gggaaattgg gcgatgatca
     1441 gtacgggaag cagcgtggcg aagggaaaca gcgccagcgc gatgagcccc ggccgatggg
     1501 caaaccccgg caaaaagcag ctcgccagga ggccaacgaa tcccgccaca atgatcgcgt
     1561 cgtgcagcac gcggtccgaa tcggcccaca actggaaaaa gccggtctgg ccatccagcg
     1621 gcgggtgcgg cacggtgtat cgcaccggca aggcgaaata gatgcccgcc aggcagacca
     1681 gggcgacgat cacagcagca gccgacaccc agtgggccgg cggcgcgggc aatcgccacc
     1741 gcagccgcga gagagccgcg caaatcgtcg tcaaaacggc gacgagcacg gcggctttcc
     1801 acagcgcgac gtgcgtcatg gcgtaaatcg aaaacaccag cggcaacgcg gtgcaggcca
     1861 acagccagaa ggagaattcg aaggtccgca cgcggccgat atttgccgcg aggaaaatgc
     1921 ccaccagcgg aatcgcgtag ggaatcgtga tcgccccaaa gccgaaagcg gcacagagca
     1981 cgagtcccgc gcgaacggcc caggcccact cgcgggagtc ggcatcgagc aattcgccca
     2041 cgaagatcat caagagggca atgccgaacg gcgtttcctt gccggtctcg aacatgccat
     2101 acacgccaaa gaacgacccg atcaacacca ggaacaggcc ggcgccgatc gcccgcggaa
     2161 agcggaaggg caacgacgcc gccagccgca tcgcggcgag cgccgcgagc aaacacagcg
     2221 gaatcttgag agccgcgccg gtggtgggca cagggatgcc gggccacgtg accgcagccg
     2281 ggaagtacaa gagagcgata tacgccggcg agccttgcga atcgccgaac gacgtgatgc
     2341 tgccgtccag cgcccggccc gtccagccga gagcggcgtt atccagtgca taaggacagc
     2401 gaaagatgcc gagcgccgca acgcccatcg ccagcaggac cacggccagc gtgagaggct
     2461 gcgccgcttc gcgggcgagc tgacgtgcaa cttccgagaa gtcacgtcgc cgccaggtaa
     2521 ccagtgcaac gagcgccgcc agcccgatca gccagagcgc cgccgtcaac cagtgcggcg
     2581 gaacgcgcca ttgcagcatc gcgggattga ccgcgaagaa ccagaccatc agccacagcg
     2641 atcccagcgc caacacgcgg ctcaccaggc acgaaagcgc ctcgccgggg cggcgcaagg
     2701 ggcggcacag cgattccagc agaaacccgg gcgccagcag gcccagcacg aggtgaatgg
     2761 cgacaaccag aaccgaaatc gtcagtgcgg ccatacggca tccttgccag caatggctgg
     2821 aggttcattt cctaacgcag tccgcgggcc gcaacccaac aggcgaggcg gcaccaaggg
     2881 ggtcgggagt caattgcggc agatgttgtc cagatggagc gccgtggccg caattgactc
     2941 cc
//
LOCUS       DASUGB010000215         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1553707, whole genome shotgun sequence.
ACCESSION   DASUGB010000215 DASUGB010000000
VERSION     DASUGB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553707"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFV87_04235"
     CDS             <1..960
                     /locus_tag="VFV87_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5102992.1"
                     /translation="GDEGNSASPSPVTDGEHVWAMMMTGDLACYTKDGQEVWKLNLQD
                     RYGKFKIQFGMTSSPVLDSDRLYLQLIHGEMRGNQQDARVVCLEADTGDEVWAVERLS
                     DGTFENKHSYASPVMYRDAERAFLLTHGNDYIVAHDLKDGHELWRCGDLNPKGNYEPT
                     LRFVASPLAVPGYIVVPTAKNRGVVCLKPGGEGDITDAAEFAHWKRDNNTPDVPSPLL
                     VDGLVYLCRENGNLMCLDANTGEEHYKDEPTTRDRHRASPVYADGHIYTTARKGVVTV
                     VKAGPKFEIVAQNDLGESMSASPAISNGRIYLRTFDALYAIGK"
     gene            1031..1417
                     /locus_tag="VFV87_04240"
     CDS             1031..1417
                     /locus_tag="VFV87_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5102993.1"
                     /translation="MNRTALGLLALLLVVIGGITLATRGPADESTTPFAAGCIRVGLV
                     LAALWLALPQIQAFFARAPRWLLVASAIGLIIGAVKPWLLLIIVPVLIALWILGPKLA
                     TKADKPFVPQKKPRRRSDATPRGRGG"
     gene            complement(1414..2259)
                     /locus_tag="VFV87_04245"
     CDS             complement(1414..2259)
                     /locus_tag="VFV87_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5102994.1"
                     /translation="MEKWPIGVFTSVDAGLGVKLEVAHELGVPTIQIHAPHKETRTQA
                     SADAFLKRLKECNIQLTCVFSGFMGESYADIPTVARSVGLVPPETRAARTAEFKEIAD
                     FAKLLGCQVLGMHIGFVPHDRTLPLYKDVVAVAREACDHCANNGQALHLETGQETADD
                     LLAFIEDVGKTNLFINFDPANMILYGTGEPIAALKRVGHFVRSVHCKDGKWAKNPGKE
                     WGQEVPLGQGDVGMEKYLRTLKEIGYSGPLTIEREIPQEPERQKAEIGHAIKLLTELK
                     AKVLA"
     gene            complement(2446..>3549)
                     /locus_tag="VFV87_04250"
     CDS             complement(2446..>3549)
                     /locus_tag="VFV87_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5102995.1"
                     /translation="YGLWALKHTYNRGIVFRDFAVPAENLLQPRRGDGLTIAYHGLNL
                     GRVSLCANAAGTMRIMMASMIPWANYRVTYGQPIARRELVRRRLGELAGLITACDALV
                     AWCSQLIDQGYRGEMECIVAKIFGSEAQKEAAIELFMKTHGGRSFLHGHLFGDHVHEY
                     LAPCIYEGEGEMLGMALFKSLVKQHGTQYFEPVGKLLVANHMKSFSPANPLHVWKLRM
                     ALVPYGLWWTGEFFARKWRTELGPMSDTLREHAIFAIRGLQAMPRQISAAMRKHQLKL
                     ADRQCRMSELSARVQKLVVMLCTSLYAAKQNDEMLRAAADVICQDLTRELTGARPTDR
                     YYRAMNTLGEQIAEGKFASIAGLKPDEILMRYK"
BASE COUNT          621 a         1113 c         1124 g          691 t
ORIGIN      
        1 ggcgatgagg gcaattccgc ctccccttca ccggtgaccg atggcgagca cgtctgggcg
       61 atgatgatga ccggcgattt ggcctgctac accaaggacg gccaggaggt ttggaagctc
      121 aacctgcagg accgctacgg caagttcaag attcagttcg gcatgaccag ctcgcccgtg
      181 ctcgatagcg accggctcta tctgcagctc attcatggcg agatgcgcgg aaaccagcag
      241 gacgcccgcg tagtttgcct tgaggctgac actggcgatg aagtctgggc ggtggagcga
      301 ctcagcgacg gaacgtttga gaataagcac tcttatgcct cgcccgtgat gtatcgcgat
      361 gccgagcggg cctttctgct gacgcatggc aacgactaca tcgtcgcgca cgatttgaag
      421 gatggccacg agctgtggcg ctgcggcgac ctcaatccca aggggaatta cgagccgacg
      481 ctgcggtttg tcgcctcgcc gcttgccgtg ccggggtaca tcgttgtgcc aacggccaaa
      541 aaccggggcg tcgtctgttt gaaaccgggt ggggaaggag acatcaccga cgccgcagaa
      601 ttcgcccact ggaaacgcga caacaacacg ccggacgtcc cctcaccgct cctggtggat
      661 ggcctcgtct atctctgccg cgagaatggc aacctgatgt gcctcgacgc caacacgggc
      721 gaggagcact ataaggatga gccaaccacg cgcgaccgcc accgggcctc gcccgtgtat
      781 gcggacggac acatttacac gaccgcccgc aagggagtcg tcaccgtggt gaaagccggg
      841 ccgaagttcg agattgtcgc tcagaacgac ctgggcgaat cgatgtcggc ctcgcccgcg
      901 atcagcaatg gccggatcta cctccgcact ttcgacgcgc tctatgcgat cggcaagtag
      961 caccttgggg tggctgtgtt ttcgccgcga aaacacagtc gcgctcagcc aggccggcta
     1021 aaatcgacgc atgaaccgta ctgctctcgg cctgctcgcg ctgctgctcg tcgtcatcgg
     1081 cgggattacg ctcgcgacgc gcggtcctgc ggacgagagc acaacaccct tcgccgccgg
     1141 ctgcattcga gttgggctag tgctcgcggc gctgtggcta gccctaccac agatccaagc
     1201 ttttttcgct cgggctccac ggtggctgct cgttgcatcg gccatcggat tgatcatcgg
     1261 cgcggtcaag ccctggctgc tcttgattat cgtgccggtg cttatcgcgc tctggattct
     1321 gggccccaag cttgccacca aagccgacaa gccgttcgtg ccgcagaaga agccgcggcg
     1381 gcggagcgac gctacgccgc gtggacgcgg cggctaagcc agaaccttcg ccttcagctc
     1441 ggtcagcagc ttgatggcat gcccgatctc ggccttctgg cgctccggct cctgcgggat
     1501 ttcgcgctcg atcgtgagcg ggccgctgta accgatttcc ttaagtgttc gcaagtattt
     1561 ctccatacca acgtcaccct ggccgagcgg cacttcctgg ccccattcct tgcctgggtt
     1621 cttcgcccat ttgccgtcct tgcaatggac gctgcggacg aaatgcccga cgcgcttcag
     1681 tgccgcgatc ggctcgccgg tgccgtagag gatcatgttg gccgggtcga aattgatgaa
     1741 caggttggtt ttgcctacgt cttcgatgaa ggccagcagg tcatcagccg tttcctgacc
     1801 ggtttccaag tgcagcgcct ggccgttgtt cgcacaatgg tcgcacgcct cgcgggccac
     1861 ggcgaccacg tctttgtaca gcggcagcgt ccggtcgtgc ggcacaaagc cgatgtgcat
     1921 tcccagcacc tggcagccga gcagtttggc gaaatcagcg atttccttga attccgccgt
     1981 gcgcgccgcg cgcgtctcgg gcggcaccaa gccgaccgag cgggcgaccg taggaatatc
     2041 ggcgtagctc tcccccatga acccgctaaa gacgcatgtg agctgaatgt tgcattcctt
     2101 cagccgtttg aggaaggcat cggcgctggc ctgcgtgcgc gtctccttgt gcggcgcgtg
     2161 aatctggatc gtcggcacgc ccaattcgtg agccacttcc agcttgacgc ccagccctgc
     2221 atcgacgctg gtgaagacac cgatgggcca tttttccatg atggggctcc gagtgaggga
     2281 gtcggggttc gggggtcggg agtcggctgt ctccggctcc gaatctacgc cgctgctgtg
     2341 ccggctgcaa cggccctaaa aagtaggtcc cgacggccgc ggcgggaccg gagccgcgag
     2401 cggatgacga actcgcgatt ctggtcccgc ccaggcggac gggacctact tgtaccgcat
     2461 gaggatctcg tccggcttca ggccggcgat ggaggcgaac ttgccttcgg cgatttgttc
     2521 gccgagcgtg ttcatcgcgc ggtagtagcg gtcggtgggc cgagcaccgg tcagttcgcg
     2581 ggtcaggtcc tggcaaatca cgtcggctgc ggcgcggagc atttcatcgt tctgctttgc
     2641 ggcgtagagg ctcgtgcaga gcatgacgac cagcttctgc acgcgggccg aaagctcgct
     2701 catccggcac tggcggtcgg ccagcttgag ctggtgtttg cgcatcgcgg cgctgatctg
     2761 ccgcggcatc gcctgcagac cgcgaatggc gaagatggcg tgctcgcgga gtgtgtcgct
     2821 catcggccct agctccgtgc gccatttgcg ggcgaagaat tcgccggtcc accacaggcc
     2881 atacggcacc agggccatcc gcagcttcca cacgtgcagc ggattagccg ggctgaatga
     2941 cttcatgtga ttggcgacga gcagcttgcc gaccggctcg aaatactgcg tgccgtgctg
     3001 cttgacgagc gatttgaaga gggccatgcc cagcatttcg ccttcgcctt cgtaaatgca
     3061 cggggccaga tactcgtgca cgtggtctcc gaacaggtgg ccgtgcagga acgaccggcc
     3121 gccgtgagtc ttcatgaaca gctcgatcgc cgcttctttc tgggcttcgc tgccgaagat
     3181 cttggcgacg atgcattcca tttcgccgcg ataaccttgg tcgatgagct gcgagcacca
     3241 cgccacgagc gcgtcgcacg cggtgatcag gcccgccaat tcgcccaggc ggcggcgcac
     3301 cagctcgcgg cgagcgatcg gctggccgta cgtgacgcgg taattcgccc acggaatcat
     3361 gctcgccatc atgatccgca tcgtgccggc ggcgttggcg caaagcgaga cgcggccgag
     3421 attcagtccg tgataggcaa tcgtgaggcc gtcgccgcgc cgtggctgca gcaggttttc
     3481 ggccgggacg gcaaagtcac gaaacacgat gccgcggttg tacgtgtgct tgagcgccca
     3541 caggccgta
//
LOCUS       DASUGB010000216         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_441146, whole genome shotgun sequence.
ACCESSION   DASUGB010000216 DASUGB010000000
VERSION     DASUGB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441146"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1551
                     /locus_tag="VFV87_04255"
     CDS             295..1551
                     /locus_tag="VFV87_04255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX5102996.1"
                     /translation="MSSHAPKNLPDSQRIAITGVGLTAPNGNNLAEFRAALLAGQSGV
                     TKYEIRYVGETLAGLCTFDALRYQTKKDLRRGTRAGSVGIYSANEAIQDSGLDWPNID
                     RSRVGIYLGVTEHGNVETENEIYLLKGYDYDTSFWSHHHNPRTVANNPAGETALNLGV
                     TGPHYTIGAACAAGNAGLIQGAQMLRLGDCDLALAGGVSESVQTFGIFASFKSQGALA
                     THDDPAKASRPFDKARNGIVVAEGGCVFVLERLDDARRRGAKIYGELAGYAINTDATD
                     FVLPNPERQAECVELAIKRAGLNPSEIDIVSTHATGTGQGDAQECQALRRAFAGSEKT
                     YFNNTKSFIGHAMGAAGALELAGNLPAFADGVCHATINVDELDPECALTTLVCNQPRE
                     IGRVEYILNNSFGMLGINSAVIIKRL"
     gene            1642..1902
                     /locus_tag="VFV87_04260"
     CDS             1642..1902
                     /locus_tag="VFV87_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5102997.1"
                     /translation="MTGPEIREEVLDILSDIAPDEDLSGLVDDKPFREQLELDSMDFL
                     DIVMELRKRHRIQVPEEDYKHLASLASTVAYLQPMLKDVVKK"
     gene            2022..3422
                     /locus_tag="VFV87_04265"
     CDS             2022..3422
                     /locus_tag="VFV87_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5102998.1"
                     /translation="MYDTLIIGAGMSGLAAGIRLAYYEQKVCILERHTTIGGLNSFYR
                     LRGRDYDVGLHAVTNYAPKGDKKGPLAKLLRQLRLKWEDFSLAPQVGSTIAFPGVSLA
                     FNNDVRLLAAEVGRAFPRQKDNFQRLLGQILDYDDLNQEVFELSARQILGEIISDPLL
                     VEMLLCPAMWYGSAREDDMDWGQFCILFRSIFLEGMGRPHAGIRLILKNLVRKFRSLG
                     GELKLRSGVSQIKTEEGRAVGVVLENGQELEARTILSSAGALETLRLCDRLASAEPVR
                     AGQLSFAESISVLDRTPDSAGFDQTAVFFNDSDKLHWHRPEAELCDVRTGVICSPNNF
                     AYAPGEGELAEGFVRITALANFDRWQALPEEQYRLEKLRWYDRMVASAVRFIPDFRSR
                     VVETDMFTPTTIRRFTGHENGAVYGAPHKQLDGTTPYKNLFLCGTDQGYCGIVGSLIS
                     GIAMANRHCLMGAAAT"
BASE COUNT          644 a         1205 c         1054 g          587 t
ORIGIN      
        1 gttcgacatc tccgccgccc gccgcgacct cggctacgag ccgcgcgtca gcatggccga
       61 aggcatgcag cggctgaaat cgtggttaac cgagagggaa gctggcggtt aactcaacgt
      121 ggggcagaca ttcctgtctg ccatctcggc aggcacgaat gcctgcccca cgctgccgct
      181 tgtcactccg ggtgcccagc ggtaacttcg gaggtttgcc tccgctattc gtaggacgga
      241 tttccaaccc gtccctcctc gtgttgcgga cggcttggaa agccatccta cgccatgtcc
      301 tctcacgcgc cgaaaaacct gccggacagc cagcggattg ccatcaccgg cgtggggctg
      361 accgcgccca acggcaacaa cctggcggag ttccgggccg ccctgctcgc cggccaaagc
      421 ggcgtgacga agtacgaaat ccgctacgtc ggcgagacgc tcgccggcct ctgcacgttc
      481 gacgccctcc gctaccagac caagaaggac ctccgccgcg gcacccgtgc cggcagcgtc
      541 ggcatctact ccgccaacga ggccatccag gacagcggac tcgattggcc gaacatcgac
      601 aggtcccgcg tcggcatcta cctcggcgtc accgagcacg gcaacgtcga gaccgagaac
      661 gagatctacc tgctcaaggg ctacgactac gatacgagct tctggtcgca ccaccacaat
      721 ccgcggacgg tggccaacaa tccggcgggc gaaaccgcgc tcaacctggg agtgaccggc
      781 ccccattaca cgatcggcgc cgcctgtgcc gcgggcaacg ccgggctgat ccagggcgct
      841 cagatgctgc gcctgggcga ctgcgacctg gccctggccg gcggcgtctc ggaaagcgtg
      901 cagacgttcg gcatcttcgc cagcttcaag agccaggggg cgctggccac gcacgacgac
      961 ccggctaaag cctcgcgccc cttcgataag gcccgcaatg gcatcgtcgt cgccgaagga
     1021 ggctgcgtgt tcgtgctgga gcgactcgac gatgcccgcc gccgcggcgc gaagatctac
     1081 ggcgagctgg ccggctatgc gatcaatacc gacgcgaccg acttcgtcct gcccaatccc
     1141 gagcggcagg ccgagtgcgt cgagctggcc atcaaacgcg cggggctgaa tcccagcgag
     1201 atcgacatcg tcagcaccca cgccaccggc accggccagg gggacgccca ggaatgccag
     1261 gccctccgcc gggccttcgc cggcagcgaa aagacgtact tcaacaacac gaaaagcttc
     1321 atcggccatg ccatgggagc agccggggcc ctggaattgg cgggcaatct ccccgccttt
     1381 gccgatggcg tgtgccatgc cacaatcaac gtggacgagt tggacccgga gtgtgccctg
     1441 acgacgctgg tctgcaatca gccccgcgaa atcggccgcg tagagtacat cctgaacaac
     1501 tcgttcggca tgctcggaat caattccgcc gtcattatca agcgcctgta gggaacgccc
     1561 tccgtggcgt ttcgatttgg aagtactgcc agcgccctcc gtggcgttct ggtttgatcg
     1621 ttccgtggag aactctgcct gatgactggt cctgaaatcc gcgaggaagt cctcgatatt
     1681 ctgtccgaca tcgcgcccga cgaggacctc tcgggcctgg tcgatgacaa gccgttccgc
     1741 gaacagcttg aactcgacag catggacttc ctcgatatcg tgatggagct ccgcaagcgg
     1801 caccggatcc aggtccccga agaggattac aaacacctcg ccagcctcgc cagcaccgtc
     1861 gcctacctgc agccgatgct caaggatgtc gtcaagaagt agcgctttgc caggaactga
     1921 ttgcaccagg tgagtaggtg agtggtgcgt tgtgagtggt ggtcgcgggc aaccatcaca
     1981 actcaccact cacaacttac cactcacgaa ttcgcccccc cgtgtacgac acgctcatca
     2041 tcggcgccgg catgtcgggc ctagccgcgg gcattcgcct ggcctattac gagcagaagg
     2101 tctgcatcct cgagcggcac acgacgatcg gcgggctgaa ctcgttctat cgcctccgcg
     2161 gccgcgatta cgacgtcggc ctgcacgccg tcaccaacta cgcccccaag ggggacaaaa
     2221 aaggcccgct cgccaagctc ctgcggcaat tgcgattgaa atgggaagac ttttccctcg
     2281 ccccgcaggt cggctcgacg atcgccttcc ccggcgtcag cctcgcgttc aataacgacg
     2341 tgcggctgct tgcggcggag gtcggccggg cgtttccgcg gcagaaagac aacttccagc
     2401 ggctgctcgg ccagattctc gactacgacg acctgaacca ggaagtcttc gagctctcgg
     2461 cccggcagat cctgggcgag atcatcagcg acccgctgct ggtcgagatg ctcctttgcc
     2521 cggcgatgtg gtacggcagt gcccgcgagg acgacatgga ctggggccag ttctgtatcc
     2581 tctttcgcag catttttctg gaagggatgg gccgcccgca cgccggcatc cggctgatcc
     2641 tgaagaacct cgtccgcaag ttccgctcgc tcggcggcga gctgaagctg cggagcggcg
     2701 tctcgcaaat caagaccgaa gagggacggg ccgtcggcgt cgtcctggaa aacggccagg
     2761 aactggaagc ccgcaccatc ctctcctcgg ccggggccct cgaaacgctc cgcctgtgcg
     2821 atcgcctcgc ctccgcggag ccggtccgcg ccggccagct ctcgtttgcc gagtcgatct
     2881 ccgtcctcga ccgcacgccg gatagcgccg gcttcgatca gaccgctgtg ttcttcaacg
     2941 acagcgacaa gctgcactgg catcggcccg aggccgaatt gtgcgacgtg cggactggcg
     3001 tcatctgctc gccgaacaat tttgcctacg cgccgggcga aggggagctt gctgaaggct
     3061 tcgtgcggat caccgccctg gcgaacttcg accgctggca ggccctgccg gaggagcagt
     3121 accgcctgga aaagctccgc tggtacgacc ggatggtcgc ctccgccgtg cggttcatcc
     3181 ccgatttccg cagccgggtg gtcgagaccg acatgttcac gcccacgacg atccgccgct
     3241 tcacgggcca cgagaacggc gccgtctatg gcgccccgca caagcagctc gacggcacca
     3301 cgccctacaa aaacctcttc ctctgcggca ccgaccaggg ctactgcggc atcgtcggct
     3361 ccctcatcag cggcatcgcc atggccaacc ggcattgcct catgggtgcg gcggcgacgt
     3421 aagcaccgta ggacggtcct cccggtccgt ccatattctc ccctcgtccc ttgcgggaga
     3481 gcggccgggg
//
LOCUS       DASUGB010000217         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_580257, whole genome shotgun sequence.
ACCESSION   DASUGB010000217 DASUGB010000000
VERSION     DASUGB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580257"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFV87_04270"
     CDS             <1..1003
                     /locus_tag="VFV87_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX5102999.1"
                     /translation="HTFTISRESITSQPTLVVELQTGGISGCGEATANAYYGFTIERM
                     AHDLAAVRSVIEALSSPDDPEGLHGKLQPLLKDDPFALCAVDQAAYDLWGKVQNLPTW
                     KLWGLSLDKIPSSDYTIGIDSIDVMVAKLNEFPGWPIYKIKLGTDRDLDIVRELRTHT
                     DANFRVDANCGWTAEQTIRNAPALKALGVEFIEQPLKADRWDEMRRVRAESALPIIAD
                     ESCIVESDVERCAGVFHGINIKLVKCGGLTPARRMIAKARELGLSVMVGCMTESTVGI
                     SAIAQLLPLLDYVDMDGAVLLAHDIASGVTLNRGRCLYPDVPGNGVTLLETGRDPLA"
     gene            complement(1117..1404)
                     /locus_tag="VFV87_04275"
     CDS             complement(1117..1404)
                     /locus_tag="VFV87_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103000.1"
                     /translation="MASRVQAQKALEIHEQKLSGCKNVVGLGIVPADEESGSKDLAVA
                     VYVKKKLPLSKLAKDQVVPETLPVPGRGKVVQVPTRVIEQDKVELEAVERV"
     gene            complement(1460..2470)
                     /locus_tag="VFV87_04280"
     CDS             complement(1460..2470)
                     /locus_tag="VFV87_04280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX5103001.1"
                     /translation="MPTEKQLVALLDMLTEGAVASAAVESVRAEAATLRKAAKPWTEG
                     KGIQGVGIGEKITDGEQLDEVVLKVYVDQKKPKSQLDNPVPKKVLMPGLDEEVSTDVE
                     EIGRMEKEPNTARIRPAVPGFGVGHVDITVGTFGCLVRKKGQKSLYILSNSHVLADEG
                     VAKIGDAIIQPGDFDGGKAPGDVIAELADWAPFQFTTTGYPNLVDAAIAKVKKAADVT
                     SAVRLIGVPKGVSKFVRRGMQVQKTGRTTDYTVGVIKDINYRLALRYKKPGGGTGRVG
                     LGDQVLCTRYTAGGDSGSAVFNMSGKVVGLHFAGSPSTSVFNRIEHVLSALDIELVTT
                     KV"
     gene            complement(2557..3894)
                     /locus_tag="VFV87_04285"
     CDS             complement(2557..3894)
                     /locus_tag="VFV87_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5103002.1"
                     /translation="MGVGLERVPSAVVERFEEAFSTEMVGRNCRLEEYRKVNVRSLRV
                     GGEMIAYCSPDSTYAATKRLLDGAKRSILIGIYDFTAKYMKELLLKAMRRGVAVSLML
                     DLDDRTGETELFNELGHHGAMVVPAPSCASEHARYFSSSHEKVIVIDDEWTLVQSGNW
                     SDASIPLNETDGGDRDDFEFGNRDMGLAIKSKPLARFFTKILKSDMQLELDSTPEALE
                     RAEEEVPGLEAFGSRPTEPAPELFRSKSFNPPGTVKVTPILSPDNYMDVIPGVLAAAR
                     KSIYIEQQYIRSSQPLIKRLLDAIGTALDEHPDLDVRIVVAPSYTSADQAKMEQVVAD
                     LRTRGMKMGDNFRLLSGKHFVHCHNKLIVVDEKVVLLSSQNWSDSAVGKNREAGLLVE
                     YPAVARHFAKIFNVDWETGKKRLVEPERLEIFGPESLASGQTVRLNWGDYAEV"
     gene            4102..>4568
                     /locus_tag="VFV87_04290"
     CDS             4102..>4568
                     /locus_tag="VFV87_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5103003.1"
                     /translation="MGPITVILDRASLGDPQAAEQLLPLVYDELRKLAAAKLVQENPG
                     QTLQATALVHEVYLRLVGGGGDREQGAGDREGRTEVRRQTPESVSGLRSPVSFQSRSH
                     FFAAAAEAMRRILIDQARKKRSLRHGGGRQRCELSDGDYITLPISDDLLDLDEA"
BASE COUNT          831 a         1507 c         1333 g          897 t
ORIGIN      
        1 gcacacgttc acgatcagcc gcgagtcgat caccagccag ccgacgctgg tcgtcgagct
       61 gcagacgggc ggcatcagcg gctgcggcga ggcgaccgcc aacgcctatt acggcttcac
      121 gatcgagcgg atggcccacg atttggcggc cgttcgcagc gtcatcgagg ccttaagcag
      181 tccggacgac ccggagggtc tgcacggcaa attgcagccg ctgttgaagg acgatccctt
      241 cgccctctgc gccgtcgatc aggccgcgta cgacttgtgg ggcaaggtcc agaacctgcc
      301 gacctggaag ctgtggggcc tgtcgctcga caagatcccg agttccgact acacaatcgg
      361 catcgactcg atcgacgtca tggtcgccaa gctcaacgaa ttccccggat ggcccatcta
      421 caagatcaaa ctcggcaccg accgcgacct ggacatcgtc cgcgagctgc gcacacacac
      481 ggacgcgaac ttccgcgtcg atgccaactg cggctggacg gcggagcaga cgatccgcaa
      541 tgctcccgcg ctcaaggcac taggcgtcga attcatcgaa cagccgctga aagccgatcg
      601 ctgggacgaa atgcgccgcg tgcgggcaga atccgcgctc ccgatcattg ccgacgaaag
      661 ctgcatcgtg gaaagcgacg tcgagcgctg cgccggcgtc ttccacggaa tcaacatcaa
      721 gctcgtcaaa tgcggcggcc tcacgcccgc ccggcggatg atcgccaaag cccgcgaact
      781 gggcctctcg gtcatggtcg gctgcatgac cgaatcgacc gtcggcatat cggccatcgc
      841 gcaattgctg ccgctgctcg actatgtcga tatggacggc gctgtgctgc tggcccacga
      901 catcgccagc ggtgtcacac tcaaccgcgg ccgctgcctc tatcccgacg tccccggcaa
      961 cggcgtcacg ctgctggaaa ctggccgcga cccgctcgcg taatgcgatt ttgcacattt
     1021 cgccccggtg cttgcaccgg gcgtcgcacg aagtgggtct ctggccggag cgcggcgcga
     1081 gcaccgccgc gaaatgcgcg cgacgccttt cgatcgctaa acccgctcca ctgcttccag
     1141 ctcgactttg tcttgctcaa tcacgcgggt cggcacttgc acgactttgc ctcggcccgg
     1201 aactggcagt gtttcgggca caacctggtc ctttgctagc ttcgagagtg gcagcttctt
     1261 tttcacatag accgcgaccg ccaggtcttt gctgccggat tcttcatccg cgggcacgat
     1321 gcctaagccg acgacgttct tgcagccgga caacttctgc tcgtgaatct ccagggcctt
     1381 ttgggcctgc acacggctgg ccatactcgt tctcctcatt tctcgcggcc aacacccgcc
     1441 ctcggacatt atacgggcgt cagaccttcg tcgtcaccag ttcaatatcc agggccgaca
     1501 gcacgtgctc gatgcgattg aagacgctcg ttgaaggcga accggcgaaa tgtaagccga
     1561 caactttccc gctcatgttg aacactgccg agccgctatc gccgccggcc gtgtagcgcg
     1621 tgcagaggac ctggtcaccc aaacccacac ggcccgtccc cccacctggc ttcttgtatc
     1681 gcagggcgag ccggtaattg atgtccttga tcacgccgac cgtatagtcg gtggtgcggc
     1741 cggtcttttg gacctgcatt ccccgccgga cgaacttgct cacgcccttc ggcacgccga
     1801 tcagtcgcac ggcgctcgtc acatcggcgg ccttcttcac cttggcgatc gccgcatcga
     1861 ccaggttcgg atagccggtg gtggtgaact ggaacggcgc ccagtcggcc agctctgcaa
     1921 tcacgtcgcc cggcgctttg ccgccatcaa agtcgcccgg ctggatgatg gcatcgccga
     1981 tcttcgccac gccttcatcc gccagcacgt gcgaattgct gagaatatag agcgacttct
     2041 gccccttctt ccgcaccagg cagccaaacg tgccgaccgt gatatcgacg tgccccacac
     2101 cgaagccggg aacggcgggg cggatgcggg cggtgttcgg ctccttctcc atgcggccga
     2161 tttcttcgac gtcggtactc acttcctcgt cgaggcccgg catgaggact ttcttcggca
     2221 cgggattgtc gagctggctc ttgggctttt tttgatcgac gtagaccttg aggacgactt
     2281 cgtcgagctg ttcgccgtcg gtaatcttct ctccgatgcc cacgccttga atgcctttgc
     2341 cctcggtcca gggcttggcc gccttccgaa gcgtggccgc ttccgcccgg accgattcca
     2401 cggctgccga ggcgacggcg ccctccgtca gcatgtccaa tagcgcgacg agctgcttct
     2461 ccgttggcat gatgttgctc ctcaaagtaa cccgaagcgt aagcgaggga gtgcggtgta
     2521 agaaggactc cctcgctgac gcttcgagtt ggtacgtcac acttccgcgt aatcgcccca
     2581 gttcagccgc acggtctgcc cgctggcaag cgactcgggt ccaaagattt ccagccgctc
     2641 gggctctacg aggcgtttct tccccgtctc ccaatcaacg ttgaagatct tggcgaagtg
     2701 cctcgctacc gcgggatatt cgaccagcag gccagcttcg cgattcttgc cgacggctga
     2761 atcggaccag ttctgcgagc tgagcagcac tactttctca tcgaccacga tcaacttgtt
     2821 gtggcaatgt acaaagtgct tgccgctgag cagacggaaa ttgtcgccca ttttcatgcc
     2881 gcgcgtgcga aggtcggcaa ccacttgctc catcttcgcc tggtcggctg aggtgtagga
     2941 tggtgcgacg acaatccgca cgtcgagatc ggggtgctca tcaagcgcgg tgccgattgc
     3001 gtccagcagc cgcttgatca gtggctgcga cgagcggatg tactgctgct cgatgtagat
     3061 cgacttccgg gccgcggcca gcacgcccgg aatcacgtcc atatagttat ctggcgagag
     3121 aatcggcgtg accttgaccg tccccggcgg attgaagctc ttgctgcgga acagctcggg
     3181 cgccggctcg gtcggacgcg atccgaacgc ttccagaccg ggcacttctt cctcggcccg
     3241 ctccagggcc tccggcgtgg aatcgagctc gagctgcatg tcgctcttga gaatcttcgt
     3301 aaagaaccgg gcgagcggct tcgacttgat tgccaggccc atgtcgcgat tgccgaattc
     3361 gaagtcgtcc cggtccccgc cgtccgtttc attcagcgga atgctggcgt cggaccagtt
     3421 gccgctttgc acgagggtcc actcgtcgtc gatcacgatc actttctcgt gcgacgacga
     3481 aaagtagcgg gcgtgctcgc tggcgcacga cggcgcggga accaccatcg cgccgtgatg
     3541 ccccagctcg ttgaacagct ccgtttcgcc cgtgcggtcg tccaggtcga gcatcagcga
     3601 cacggccacg ccccgccgca tcgccttgag gagcagctcc ttcatgtact tggcggtgaa
     3661 gtcgtagata ccgatcaaga tcgaccgttt cgcaccatcc aaaagccgct tcgtcgccgc
     3721 ataggtggaa tcgggcgaac aataggcgat catctcgccg ccgacgcgca gcgagcgcac
     3781 attcactttg cgatattcct ccagccggca gttgcggccg accatttcgg tggagaacgc
     3841 ttcctcgaac cgctcgacga ccgcgctcgg cacgcgctcc agcccgacgc ccatggcaaa
     3901 gctcccgcgg gtagtgaaaa atcccccaca ctgccgcgaa cagaaagtct aaccgtcttt
     3961 tactcataac tgccaccatc agccgtcaac tttgcggcag accgcaaaac accagagtga
     4021 atgccggcct gccattgcga ccacaattcg ctgtttgcgt cttgtttgct ttgcccgcgc
     4081 caagcaccta tcgtgacgct catggggcct atcactgtca ttctggatcg agcttccttg
     4141 ggggatcccc aagccgccga gcaacttctg ccgctggtct acgacgagtt gcgaaagctg
     4201 gcggcggcga aactggtgca ggagaatccc ggccagacgc tgcaggcgac ggccctggtc
     4261 cacgaggtgt atctgcggct ggtgggtggc ggaggggaca gagaacaggg ggcaggggac
     4321 agggaaggga gaacggaggt ccgacgtcag acgccagagt ctgtctccgg tctccggtct
     4381 cctgtctcct tccagtcccg cagccacttc tttgccgccg cggccgaggc gatgcgacgc
     4441 atcctgattg accaggcacg gaaaaagcga agcctccgtc atggcggggg acgccagcgt
     4501 tgtgaattgt ccgacggcga ttacatcacg ctccccatca gcgatgatct gctcgacctc
     4561 gatgaggc
//
LOCUS       DASUGB010000218         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_719292, whole genome shotgun sequence.
ACCESSION   DASUGB010000218 DASUGB010000000
VERSION     DASUGB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719292"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFV87_04295"
     CDS             <1..1464
                     /locus_tag="VFV87_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5103004.1"
                     /translation="EAQTLARLDHPAIVPVFDVGQTDRGEHYVVAKYIRGCSLAERIA
                     AGRIAPRDAAAIIARVCDGLHHAHQQGIVHRDIKPANILLDERGEPLVADFGLALHEL
                     EQLAKRGEVAGTPAYMSPEQVRGQAHHLDGRSDLWSVGALLYEMLAGQRPFTGSLSAD
                     LFDEIAHREPKPLRLIDPSIPQRLEEIVGRCLRKNVADRYATAADVTADLRAWLAEEQ
                     PLEQGKSQEKRRDKRTYVIAGIATACVAALLLIAAWPWLPQRPQIQISSDAVVVQTSP
                     IESEDSIAPALPPEQLDGSVSLRIWSPIDSSRRGRLLDEPGMLPLVAGDQIRVEATLS
                     QAAFAYLVWIDVAGQATPVYPWRPGDWNSRPTSEEKVLRLSLPEQQDAGWEWEPASGM
                     ETLVLLARSEPLPAEVELSVQLGELPAQPQQSERTLVWLDDGRLVNELTRSPRFFDPQ
                     RIDDPLLQAQRVLQERLSPHFSLIRAISFASSGSTEP"
     gene            1461..>2514
                     /locus_tag="VFV87_04300"
     CDS             1461..>2514
                     /locus_tag="VFV87_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103005.1"
                     /translation="MTVSRTLRFSSAPLIAALALWAAAPSPAAEPLAPNRLEQLLAEA
                     ARLDAAGKLAESLPLRKEIVELAVKQHGADSLDAAHALAALAHNYRRREAFAEAEELA
                     RQALAIFQQKLGEKDLQTINTYGQLVDILNHAKKFKEAEPLGRKLVALRKEILGADQA
                     ATAESYVMLGGTLFFLEQGAEAAALHQRAFEIFSARLGPDDERTRRAEGSCADSLNVE
                     IQRRYLAAMKRHPGDDLAAQYETFEGMLDVMRQVFPPERHPHGSRRTARILMRLGELR
                     AGQGRHAEARPWLEKALAMQEKLLLAEYPEGHPELIVTHSRLSKTLHSHGEYKQASAH
                     ARQALTMARACYPTSEY"
BASE COUNT          455 a          813 c          830 g          416 t
ORIGIN      
        1 gaggcgcaaa cattggcccg gctggaccat ccggcgatcg tgccggtgtt cgacgtgggg
       61 cagaccgacc gcggcgagca ctacgttgtc gccaaatata tccgcggctg ttcactggcc
      121 gagcggattg cggcggggcg catcgctccg cgggatgccg cggcgatcat tgcccgcgta
      181 tgcgacggcc tgcatcacgc tcaccagcag ggcatcgtgc atcgcgatat caagccggca
      241 aacatcctgc tcgacgagcg cggcgagcca ctggtggccg atttcgggct ggcattgcac
      301 gagttggagc agctggcgaa gcgcggcgaa gtggcgggaa cgccggcgta catgtcgccc
      361 gagcaggtcc gcggccaggc ccaccatctc gacggccgca gcgacctctg gagcgttggc
      421 gccttgctct acgagatgct cgccggccag cggccgttta ccggaagttt gagcgcggac
      481 ctgttcgacg agattgccca tcgcgagccg aagccgttgc ggctgatcga tccgagcatt
      541 ccgcagcggc tggaagagat tgtcggccgt tgtctacgca agaatgttgc cgaccgctac
      601 gcgactgccg ccgatgtgac agcggatctg cgggcctggc tggcggagga gcagccgctc
      661 gaacagggga aatcgcagga gaaacgtcgc gacaagcgca cgtacgtcat tgccgggatc
      721 gccacagcgt gtgtcgcagc cctgctgctg atcgccgcgt ggccgtggct tccgcagcgc
      781 ccccaaattc agatttccag cgacgcggtg gtcgtgcaga cctcgccaat cgaatccgaa
      841 gattcaatcg ctcccgcatt gccccccgaa cagcttgacg gcagcgtcag tctgcggatc
      901 tggtcgccga tcgactcttc tcgccgcggc cgattactcg atgaaccggg gatgctcccg
      961 ctggtggcag gcgatcagat tcgcgtcgag gcgacgcttt cgcaggcagc gtttgcctac
     1021 cttgtctgga ttgatgtcgc gggccaagcg acgccggtct atccttggcg cccgggcgac
     1081 tggaattcgc ggccgacgtc tgaagaaaag gtgttgcggt tgtcgctccc cgagcaacaa
     1141 gacgcgggct gggagtggga gccggcttca gggatggaga cgctcgtgct gctggctcgc
     1201 tccgagccgc tgcccgcgga ggtcgagcta tcggttcagc tgggcgaatt gcccgcccag
     1261 ccgcagcaaa gcgagcgaac cctggtctgg ctcgacgatg ggcgcctggt caacgaactg
     1321 acgcggtcgc cgcgcttctt cgacccgcag cgcatcgacg atccgctgct gcaagcgcag
     1381 cgagtgcttc aagaacgtct ctctccgcac ttctcactga ttcgcgccat cagcttcgcc
     1441 agcagcggca gcaccgagcc atgaccgtat cccgaaccct ccgattctcg tccgccccgc
     1501 tgattgctgc actcgcactg tgggccgccg ccccgtctcc cgccgcagag cccctagccc
     1561 ccaaccgcct cgagcagctt ttggcggaag cggccaggct cgacgcagcc ggcaagctgg
     1621 ccgaatcgct gccgctgcgc aaggagatcg tcgaattggc ggtgaagcag cacggcgcgg
     1681 actcgctcga cgcggcccat gccctggcgg cactggccca caactatcgg cgccgagaag
     1741 catttgccga agcggaagaa cttgctcggc aggccctggc catctttcag caaaaactcg
     1801 gcgagaagga tctgcagacg atcaacacct acgggcaact cgtcgatatt ctcaatcacg
     1861 cgaagaaatt caaggaggct gagcccctgg ggcgaaagct cgttgccctg cgtaaggaaa
     1921 tcttgggtgc ggatcaggcg gcgaccgctg aaagctacgt catgcttggc ggcacgctgt
     1981 tcttcttgga gcaaggcgcc gaagcagcgg cgctgcacca gcgggccttc gagatctttt
     2041 cggcccgctt gggaccggac gacgagcgca cgcgccgcgc ggaggggagc tgcgctgaca
     2101 gcttgaatgt cgaaatccag cgccgctatc tcgccgccat gaaacggcat cccggcgacg
     2161 atctggccgc tcagtacgag accttcgagg gaatgctcga cgtgatgcgg caggtgtttc
     2221 cgccggagcg gcatccccac ggcagccggc ggacggctcg gattctgatg cggctgggcg
     2281 agctgcgggc cggacaaggg cgtcatgcag aggcccggcc gtggctggaa aaggcgctgg
     2341 cgatgcagga gaagctgctg ctggccgagt atccagaggg acatccggag ctgatcgtga
     2401 cgcactcgcg gctgagcaaa acgctgcact cgcacggcga atacaagcag gccagcgcgc
     2461 acgcccgcca ggcgctgacg atggcccgag cgtgctatcc gacatccgag tatc
//
LOCUS       DASUGB010000219         3385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1831468, whole genome shotgun sequence.
ACCESSION   DASUGB010000219 DASUGB010000000
VERSION     DASUGB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831468"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VFV87_04305"
     CDS             <1..1218
                     /locus_tag="VFV87_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5103006.1"
                     /translation="RGRLISPLVNEQRLQTERFTGWMAKRFNDNDGWDRIVFDLLTAT
                     GTIEQNPSVTYLIEGRNPLSVTDLTDLSSRYFLGVRLNCAQCHDHPFVDWKQRDYWGM
                     AAFFAQIQTPGRAKMVYLAGVQDNPKMTLAVLKESDVIEGYQPAEPTFLGGEAWKGKS
                     ATPHREALARWITSPENPYFARAMVNRLWWQFFGRGIVNPVDDMHSANAPSHPELLEL
                     LSRRFVESKFDVKFLCRSIMTSRTYQQTSKPGKQPEREAELFARMSIKVLTAEQLYDS
                     LVTVLGPPSKSPSINTRLGARYEFCQYFAGDGESDPTRYDRGIPHVLRLMNSSQFAGR
                     NASALANRIADSSRSADEVADELFLAILSRRPTAAERQIVTGAAASGDSDRDAYAELA
                     WALLQSSEFTLNH"
     gene            1236..2504
                     /locus_tag="VFV87_04310"
     CDS             1236..2504
                     /locus_tag="VFV87_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103007.1"
                     /translation="MPTFSRRGFLSLAAAGSACGWFGELAARAAEQATGKSCILLWMA
                     GGPSHIDTFDLKPEARDNIRGEFRPIDTSVPGIQISEHFPQLSRQMEHAAVLRGMNTV
                     ESDHQLASYHLHTGYQKRAGGIAFPSLGAIAARELARRDFPLPGYVCIGGGSKFATRS
                     GFLGPDHQPLDVTDPERGTDFLEPLASRGQFARQYDLLQRLDSPFQEAYRAEAARAHA
                     AALERAVRLMNADQKQAFDLTLEADKDRDRYGRGSFGQGCLMARRLIETGVRFVEVMS
                     GDGVGWDTHRDNFPRTRALSLEADLGMAALIDDLDERGLLDSTLIIWMGEFGRSPQTT
                     SGGGRNHWARAWSTVLAGGGIRGGQAIGRTDRDAAEVIERPISVVDFLATVCTVLGID
                     YKHQNRATGVQRPIPIVDTSKEITVISELL"
     gene            2589..>3385
                     /locus_tag="VFV87_04315"
     CDS             2589..>3385
                     /locus_tag="VFV87_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX5103008.1"
                     /translation="MPGGYWPWIIVLGFKFVTNEEGKSMSRLPSLIASCIWVLLAGGA
                     LSLATAEELHSGVRGLATKAPADMKIDGDLTEFRGAFCTPVNYFHPQVGERAAQFFYL
                     WDDEAFYAGLRTLDTKQFNQAPNDRLWEGDGVEWYFDTRRDENFRGIDWGKGAVHCYW
                     TGYNKAEVSPRFCLRPGYLDAIEKIGVQVAARKTEYGAEVEFKLPWANFPNFKPAAGQ
                     VIALDAELCYSDGGPRVDRIFAYGSPLCVQQPAAQGQVQLVDKLEPD"
BASE COUNT          604 a         1035 c         1152 g          594 t
ORIGIN      
        1 cggggccggc tgatttcgcc gttggtcaac gagcagcggt tgcagacgga gcggttcacc
       61 ggttggatgg cgaagcgatt caacgacaat gacggctggg accggatcgt cttcgacctg
      121 ctgacggcga ccggcacgat cgagcagaac ccgtcggtga cctatttgat cgaggggcgc
      181 aatccactct cggtcaccga tctgaccgac ctcagttcgc gatattttct gggcgtgcgg
      241 ctgaattgcg cccagtgcca cgatcatccg ttcgtcgatt ggaagcagcg cgattattgg
      301 ggcatggcgg cgttcttcgc ccagattcag acgccgggcc gggccaaaat ggtctatctg
      361 gccggcgtgc aggacaaccc caagatgacg ctcgccgtgc tcaaggaatc ggacgtgatc
      421 gaaggctatc agccggccga gccgacgttc ctgggaggcg aagcgtggaa aggcaagtcc
      481 gccacgccgc atcgcgaggc cctcgcccgg tggatcacct cgcccgaaaa tccgtacttc
      541 gcccgggcga tggtcaaccg cctgtggtgg caattcttcg gccgcggcat cgtgaatccc
      601 gtggacgaca tgcactcggc caacgcgccg tcgcatcccg aattgctcga gctgctcagc
      661 cggcggttcg tcgagtcgaa gttcgacgtg aagttcctct gccgctcgat catgaccagc
      721 cgcacgtatc agcaaaccag caagcccggc aaacagcccg agcgcgaagc ggaattgttc
      781 gcccggatgt cgatcaaggt gctgacagcc gagcagcttt acgattcgct ggtcacggtg
      841 ctgggcccgc cgagtaagag cccgtcgatc aacacgcggc tcggggcgcg atacgaattc
      901 tgccagtact ttgcgggcga tggcgaaagc gacccgacgc gctacgatcg cggcattccg
      961 cacgtgctgc ggctgatgaa ttcgtcgcag ttcgcggggc ggaatgcgtc ggcgctggcc
     1021 aatcgtattg ccgattccag ccgctcggcg gacgaagtcg ccgatgagct gttcctggcg
     1081 atcctatcgc gccggccgac cgcggccgag cggcagatcg tcacgggcgc ggccgcttcc
     1141 ggcgattcgg accgcgatgc ctacgccgag ctggcttggg cgctattgca aagcagcgaa
     1201 ttcacgctca atcactaaca gtcaaggaac ccgtcatgcc cactttttcc cggcgcggat
     1261 ttctttcgct ggctgccgcg ggcagcgcat gcggctggtt cggagagctg gcggcacggg
     1321 ccgccgagca ggcgaccggc aaatcgtgca tcctgctctg gatggccggc gggccgagcc
     1381 acatcgacac gttcgacctc aagccggagg cccgggacaa catccgcggc gaattccggc
     1441 cgatcgacac ctcggttccc ggcattcaga tcagcgagca ctttccgcag ctttcgaggc
     1501 agatggagca cgcggccgtg ctgcgcggca tgaatacggt ggagtccgat catcagctcg
     1561 ccagctatca cctgcacacg ggctatcaaa aacgggccgg cggaatcgcg tttcccagcc
     1621 tgggagcgat cgcggcgcgg gagctggccc ggcgcgattt tccgctgcct ggctatgtct
     1681 gcatcggcgg aggatcgaag ttcgccacgc gcagcggctt tctggggccc gatcaccagc
     1741 cgctcgatgt gaccgatccc gagcggggga ccgatttcct ggagccgctg gccagtcggg
     1801 gccagtttgc gcggcaatac gacctgctcc agcggctgga ctcgccgttt caggaggctt
     1861 accgggctga agcggcgcga gcccacgccg cggccctgga acgggccgtg cggctgatga
     1921 atgccgacca gaagcaggcg ttcgacctta cgctggaggc cgataaggac cgcgaccgtt
     1981 acggtcgcgg gagctttggc cagggctgcc tgatggcccg gcggttgatc gagacgggtg
     2041 tgcggtttgt ggaggtgatg agcggcgatg gcgtgggctg ggacacgcat cgcgacaatt
     2101 tcccccgcac gcgggcgctc tcgctggaag cggatctggg gatggcggca ctgatcgacg
     2161 atctggacga acgcgggctg ctcgattcga cgctgatcat ctggatgggc gaattcggcc
     2221 gcagcccgca gaccaccagc ggcggcggac gcaaccactg ggcccgggcc tggagcacgg
     2281 tgctggccgg cggcggcatc cgcggcggac aggcgatcgg ccgcaccgat cgcgatgcgg
     2341 cggaagtgat cgagcggccg atcagcgtcg tcgatttcct ggcaacggtc tgcacggttc
     2401 tcggcatcga ctacaagcat caaaaccgcg ctaccggcgt tcagcggccg attccgatcg
     2461 tcgataccag caaagagatc accgtgatct ccgagctgct gtgaggcaat tctgcgacgc
     2521 gggtggagcc ggcttgattt tccaatccgg tccaaacgga gcgacgcctg tggcgagggg
     2581 aaggggtgtt gcccggcggc tattggccgt ggatcatcgt tctcgggttc aagttcgtca
     2641 cgaacgaaga ggggaaatcc atgtcacggc tgccgagtct gattgcgagt tgcatctggg
     2701 tccttctcgc aggtggagcg ctttctctgg cgactgccga ggaactgcac tccggggttc
     2761 gcgggctggc gacgaaggcg ccggcggata tgaaaatcga cggcgacctg acggagttcc
     2821 gaggcgcctt ttgcacgccg gtcaattatt tccatccgca ggtcggcgag cgggcggccc
     2881 agttcttcta cctgtgggac gacgaagcgt tttatgccgg cctgcggacg ctcgacacga
     2941 aacaattcaa ccaggcgccg aatgaccggc tgtgggaggg ggacggcgtc gagtggtatt
     3001 tcgacacgcg ccgcgatgag aacttccgcg gaatcgactg ggggaagggc gccgttcact
     3061 gctactggac gggttacaac aaggccgagg tgagcccgcg gttctgcctg cggcccggct
     3121 acttggacgc catcgagaag atcggcgtgc aggtcgcggc gcggaagacg gaatacgggg
     3181 cggaagtgga gttcaagctt ccctgggcga attttcccaa cttcaagcca gcagcgggcc
     3241 aggtgatcgc cctcgacgcg gagctgtgct acagcgacgg tggcccgcgg gtcgatcgga
     3301 tctttgctta tggcagcccg ctgtgcgtgc aacagccggc cgcccagggc caggtgcagc
     3361 tcgtcgataa gctggagccg gacca
//
LOCUS       DASUGB010000220         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_302337, whole genome shotgun sequence.
ACCESSION   DASUGB010000220 DASUGB010000000
VERSION     DASUGB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302337"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VFV87_04320"
     CDS             complement(<1..1584)
                     /locus_tag="VFV87_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5103009.1"
                     /translation="MVRYLLDHLVSFRSSGKWLLLATLVGIVAGLGGIAFQVTEQTIF
                     RFSSQGVAGFSPKEAAGERRLFVNPNVPFSPVKLLIVLAVGGLAAGILVQAFAPEAEG
                     HGTDAAIEAYHQKRGYIRPIVPFIKLIASAITIGTGGSGGREGPIAQIGSGFGSLLGT
                     RLKLSARDRRILLAAGMGAGIGSIFRAPLAGALFAAEILYRDADLETEVIVPAAVAAI
                     VGYSVFSFWLPPEVRFLPLFGNELRHSVRSLVELVPMTVLAVGLVVVGVLYIKTFYGL
                     HNLFKRIPVPAFVRPVIGALLAGFIALALYYAWGQNPGALAVLGTGYGTLQQALVKPE
                     SVGLWLLLAIALVKIVTTSMTIGSGGSGGVFGPSMVIGGCTGGAIGAFLHDWWPKLVP
                     NPAIYAVVGMAGFFAGIAHAPISTIVMVSELTGDYSLLVPTMWVSTLCFVLCQRWTLY
                     DKQVASRLDSPAHRGDFLIDVLEGIRVKDVPWKERQTVPEGMSLKEIVHMLAENRQHY
                     FPVVDAGGKFIGIFSTDDVR"
     gene            complement(1715..>2382)
                     /locus_tag="VFV87_04325"
     CDS             complement(1715..>2382)
                     /locus_tag="VFV87_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5103010.1"
                     /translation="YDEPADGRYLLHIGGGYDFSALTQDPVLGQKLYRSTAIPEFFVG
                     DPAAPPLASALRTPVFVDTGPVPANDFHLFGLELAGVYGSFYFQAEYMQTMVNQIGGP
                     VLTFDGAYAQAGWFLTGEHRPYNRAAGVFDRLRPYEDFFSFDGLCCGSGAWELAVRWS
                     YIDLNSQNVTGGNLNDLTLGLNWYLNPFSRLQFNYIRAMADGLGPAAGNTDIYALRWQ
                     VDF"
BASE COUNT          520 a          730 c          780 g          352 t
ORIGIN      
        1 gcggacatcg tcggtggaga aaatgccgat aaacttgccg cccgcatcga cgaccgggaa
       61 ataatgctgg cggttttcgg ccagcatgtg gacgatttcc ttcagcgaca tcccttcggg
      121 aacggtctgc cgctccttcc acggaacgtc cttgacgcga atgccttcca gcacgtcgat
      181 caagaaatcg ccgcggtggg ccggcgaatc gagccgcgac gccacttgct tgtcgtacag
      241 cgtccaccgc tggcagagaa caaagcagag cgtcgaaacc cacatcgtgg gcaccaggag
      301 gctgtaatcg cccgtcagtt cgctgaccat cacaatggtg gagatcggcg cgtgggcgat
      361 gccggcgaag aagcccgcca tcccgacgac ggcgtagatc gccggattgg gcacgagctt
      421 cggccaccaa tcgtgcagaa atgctccgat cgcgccgccc gtgcagccgc cgatgaccat
      481 cgacggaccg aaaacgccgc ccgagccgcc ggatccgatt gtcatcgaag tcgtcacaat
      541 cttgaccagg gcgatggcca ggaggagcca caagccgacc gattccggct tgacgagggc
      601 ctgctgcagc gtgccatagc cggtgccgag cacagcgagg gcgcctgggt tctgccccca
      661 ggcgtaatac aaggccaggg cgatgaatcc ggccaaaaga gcgccgatga ccggccgcac
      721 gaaggccggc accggaatcc gtttgaacaa gttgtgcagg ccgtagaaag tcttgatata
      781 gaggacgccc acgacgacca gccccacggc gagcacggtc atgggcacga gttcaacgag
      841 cgagcggacg gaatgacgca actcgttgcc gaagagcggg aggaagcgca cttccggcgg
      901 cagccagaag gaaaacacgc tatagccgac gatggcagcg accgcagcgg gcacgatcac
      961 ctcggtttcc aggtcggcgt cgcgataaag gatttccgcg gcgaacagcg cgccggccag
     1021 cggagcgcgg aagatggagc cgatcccggc tcccatgccc gctgccagca gaatgcgacg
     1081 atcgcgggcc gagagcttca gccgggtacc aaggagcgag ccaaaacccg agccgatctg
     1141 ggcgatcggt ccttcgcgac cgccggagcc gccggtcccg atcgtaatgg cggacgcgat
     1201 cagcttgatg aacgggacga tggggcggat gtagccccgc ttttggtgat aggcctcgat
     1261 agccgcatcg gtgccgtgac cttccgcttc gggggcgaag gcttgaacga ggatgccggc
     1321 cgcgagcccg ccgacggcga gcacgatgag gagcttgacc ggcgaaaagg ggacgttggg
     1381 gttgacgaag agtcgccgct ccccggctgc ttccttcggg gagaaaccgg ccactccctg
     1441 cgacgagaag cggaagattg tctgctccgt cacctggaag gcgatgccgc ccaggcccgc
     1501 gacgattcca accaacgtcg ccagcagcag ccacttgccg ctggatcgaa aggaaacaag
     1561 gtgatcgagg aggtagcgta ccaaggattt aaaatgtgac ggggcgaagc tcgcaggcgt
     1621 gcctgatacg cccaaaagag aataacacct tcgcctcgtg cccacaatga agcgccgcaa
     1681 ggggagttgt gcccaatcca gcccgccatc cgcgctaaaa atcgacctgc caccgcaaag
     1741 cgtagatatc cgtattgccg gcggcgggac ccaggccatc ggccatggcc cggatgtaat
     1801 tgaattgcag cctggaaaag ggattcaggt accagttgag ccccagcgtc aggtcgttga
     1861 gattgccgcc cgtgacgttc tggctgttga ggtcgatgta cgaccaacgg acggcaagct
     1921 cccaggcgcc gctgccgcag caaaggccat cgaagctgaa gaaatcctcg tagggccgca
     1981 ggcggtcaaa cacgccggca gcgcggttgt acggccggtg ctcgcccgtc agaaaccagc
     2041 cggcctgggc ataggcgccg tcgaaggtca gcaccggccc gccgatctgg ttgaccatcg
     2101 tctgcatgta ttccgcctgg aaatagaacg aaccatagac gcccgccaat tcgaggccaa
     2161 agaggtggaa gtcgttggcg ggaaccgggc ccgtatcgac aaagaccggc gtgcggagcg
     2221 cgctggcgag cggcggcgct gcgggatcgc cgacgaagaa ttcaggaatt gccgtcgagc
     2281 ggtacagttt ctggccgagc acgggatcct gcgtcagggc ggagaagtcg tagccaccgc
     2341 cgatgtgcag gagatagcgc ccatcggccg gttcgtcgta cc
//
LOCUS       DASUGB010000221         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1275290, whole genome shotgun sequence.
ACCESSION   DASUGB010000221 DASUGB010000000
VERSION     DASUGB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275290"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFV87_04330"
     CDS             <1..287
                     /locus_tag="VFV87_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103011.1"
                     /translation="KHGIQAHVQGLGARFGVYFGIPGQVRSYRDAVQHQREQMLRFIK
                     TAIDHGVYFHDYGGAACHHGFCHAMTLENVAETLGRLDTAVAAFARTRVG"
     gene            333..1823
                     /locus_tag="VFV87_04335"
     CDS             333..1823
                     /locus_tag="VFV87_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5103012.1"
                     /translation="MRIGIGQLWQETNTFNPLPTTRRDFEEFGVLRGSELIEQLANTN
                     EPGGFIQSLRAWPEQPELAGLVRLPAWPSGTATAEAFDWLLGEVLASLRGAGRLDGVL
                     LALHGAMVAEEHPDVEGEVLQAVRREIGASVPLVATLDLHANVTERMVRHADALVLYH
                     TAPHIDVFETGVRAANVLRQIMIGGTQPKAHFRKIPLVVPAERANTQDAASVSFAFRE
                     RLQALEREPGILAAGIATVQPWLDIPELGSAVLVVAEGDNPATQTACDRLASELWQRR
                     RDYLPELTPLPKAVHLARECREGLVVLSDSADATTSGAPGDSTWLLGELLKYEWPRGA
                     LVTLVSPAAVDLAAQAGAGYSRSFTLGGVRDTRFSKPLDLPGATIERLFDAQFTMSGH
                     LGKNLAIDMGRSAVLRQGDVRIIVTSRSGPHFAPELFQAAGIDPFAAHVLVAKSPCGF
                     RAVYASRAAKIMVVQAPGCAPSDFWNYEYRNIPRPLWPWDEFEWQP"
     gene            complement(1827..2885)
                     /locus_tag="VFV87_04340"
     CDS             complement(1827..2885)
                     /locus_tag="VFV87_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5103013.1"
                     /translation="MLAAWMLAALFPAVSSAEPANAKVQRFARSELHMGVEFEVVVYA
                     ADAEVAKMATGRAFVRVAALDKALSDYDLESELSRLSATSTVADSRAAAPAGGFPAVK
                     LSDDLWTVLSFSQEVSEKSGGAFDMTVGPLTKLWRRARRQKALPEPELFNPAKAAVGY
                     RFLQLDAMERTARLRKPNMRLDLGGIAKGFAADEALAEIKRLGIAQALVRASGDIAAG
                     DPPPGEAGWLVGIAPLDPDEPPTRFVRLANMAVSTSGDSRQHLEIDGQRYSHIVDPRT
                     GLGVLGKSSVTVIAARGIAADSLATAVSILGPDQGLDLVAKYKGAALLMVADDGKGNL
                     REVASPSFGGFEIRPLKE"
     gene            2977..4047
                     /locus_tag="VFV87_04345"
     CDS             2977..4047
                     /locus_tag="VFV87_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103014.1"
                     /translation="MPRLHYALAVPVFLACLSAGWGQIALSPQAGLLVLRNGQVLEGN
                     VTRAGDYYIVTLGGTSEIRLPAADVEAVCGSLDEAYEFKLRHLSGSGARPHLELAEWC
                     LRQQLHARCAEQLVSAMRLEPQNPTLKLLERRLEFAALPPKTDQPKTAAASEFVGTEQ
                     VEKTIRALPGGSVEKFAAVVQPILLNRCGANQCHGPNSKAEFRLLRPAPGQLASRRFT
                     ERNLYAALQQLDSSHPDSSPLVALPQQRHGSSLTAVFDKHSQQQLDELVAWVKLTLAA
                     PQSNPATIAVSSPTLSQAASATATPPATPKDNAAAAAAAAVRALKPAADSSPSSPPAD
                     PNHFVPRDRFDPEIFNRRFPKS"
BASE COUNT          673 a         1377 c         1364 g          723 t
ORIGIN      
        1 agaagcacgg catccaggcg cacgtgcagg ggctgggggc ccggttcggc gtctatttcg
       61 gcattcccgg acaggtgcgg agctatcgcg acgcggtgca gcaccagcgg gagcagatgc
      121 tgcggttcat taagaccgcg atcgatcacg gcgtttattt ccacgattac ggcggggcgg
      181 cgtgccatca tggcttctgc cacgcgatga cgctggagaa cgtggcggag accctcggac
      241 gactcgatac cgcggtagcc gcgttcgcca gaacgcgggt gggctgacgg tcgcccgcac
      301 tctggcgagt gcggctacgc actgaattcg atatgcgaat cggcattggc cagctctggc
      361 aggagacgaa cacgttcaat ccactgccga cgacgcggcg ggactttgag gagttcggcg
      421 tcctccgtgg ctcggagctg atcgagcagc tcgccaacac gaacgagccg ggcggcttta
      481 tccagtcgct gcgcgcgtgg cccgagcagc cggagctcgc cggccttgtg cgactgccgg
      541 cctggcccag cggaacggcg acggccgaag cgttcgactg gctgctcggc gaggtgctcg
      601 cgtcgcttcg cggcgcgggc cgactcgacg gcgttctgtt ggcactgcac ggggcgatgg
      661 tggcggagga gcatcccgat gtggagggcg aggtgctcca agccgtgcgg cgcgaaatcg
      721 gcgccagcgt gccactggtg gcgacgctcg acctgcacgc gaatgtgacc gagcggatgg
      781 tccgtcacgc cgacgccttg gtgctctatc acacggcgcc gcatatcgac gtgttcgaga
      841 cgggcgtccg cgcggccaac gtcctgcggc agatcatgat cggcggcacg cagccgaaag
      901 cgcatttccg gaaaattccg ctcgtcgttc cggcagagcg ggccaatacg caggacgcgg
      961 caagcgtcag ctttgcgttc cgtgagcgcc tgcaagctct ggagcgagag ccgggaattc
     1021 tggcggcggg catcgccacc gtccagccgt ggctcgatat tccggaattg ggctcggccg
     1081 tgcttgtcgt ggcagagggc gataatccag cgacacagac ggcctgcgac cggctcgcat
     1141 ccgagttgtg gcagcgacgc cgcgattact tgcctgagct cacgccgctg cccaaagcgg
     1201 ttcacctggc ccgtgaatgt cgcgaggggc tggttgttct gagcgattcg gctgatgcga
     1261 cgacttccgg cgcgccgggc gattcgacgt ggctgctggg cgaactgctc aagtacgagt
     1321 ggccgcgcgg ggcgctggtg acgctggttt cgcccgcagc ggtcgacttg gcggcacagg
     1381 cgggtgctgg ctattcgcga agttttacgc tcggcggcgt tcgcgacaca cgcttttcaa
     1441 agccgctcga cctgcccggc gcgacgatcg agcggctgtt cgacgcccag ttcacgatgt
     1501 ccggccacct cggcaagaac ctggcaatcg acatggggcg ctcggcggtg ctgcggcagg
     1561 gcgacgtgcg gattatcgtc acgtcgcgca gcggaccgca tttcgcaccc gagctgtttc
     1621 aggcggcggg catcgatccg tttgccgccc atgtgctcgt ggccaagagc ccgtgcggtt
     1681 ttcgggccgt gtatgcctcg cgggcggcca agatcatggt cgttcaggcg cccgggtgtg
     1741 ctccatcgga tttctggaac tacgagtacc gaaacatccc gcgtccgctg tggccctggg
     1801 atgaattcga gtggcagccg tagtgttcac tctttgaggg gccggatttc aaagccgcca
     1861 aagctgggcg acgcgacttc gcgaagattg cctttgccgt cgtcggcgac catcagcagc
     1921 gcggcgcctt tgtatttcgc caccaggtcg aggccttggt ccggtccgag aatcgaaacg
     1981 gctgtcgcca ggctgtcggc ggcgatgccg cgcgctgcga tgacggtaac gctgctcttg
     2041 ccgaggacgc ccaggcccgt ccgcggatcg acgatgtggg aatagcgctg gccgtcgatt
     2101 tccaggtgct gccgcgagtc gccggaagtc gaaacggcca tgttcgccag ccgtacgaag
     2161 cgcgtgggcg gctcgtcggg atcgagcggg gcgatgccca ccagccagcc ggcttcgccc
     2221 ggcggcggat cgccggcggc aatgtcgccg ctggcccgca cgagagcctg tgcgattccc
     2281 aggcgcttga tctccgccaa tgcctcgtcc gcggcgaatc ccttggcgat gccgcccagg
     2341 tccagccgca tgttcggctt tcgcaggcgg gcggtgcgct ccatcgcgtc gagctgcaga
     2401 aaccgatagc cgaccgctgc tttggctgga ttgaagagct cgggctcagg cagggctttt
     2461 tgccgccgcg cacggcgcca caatttcgtg agcgggccga ccgtcatgtc gaaggccccg
     2521 ccgctcttct cgcttacttc ttgcgaaaac gacaagaccg tccacagatc gtcgctgagc
     2581 ttgacggctg gaaatccgcc tgccggtgcc gcggcgcggg aatccgcgac agtcgaagtc
     2641 gcgctaagcc ggctgagctc gctctcaagg tcgtaatcgc tgagggcctt gtcgagcgcg
     2701 gccacgcgga cgaacgcccg gcccgtggcc attttcgcga cttccgcgtc cgcggcgtac
     2761 acgacgacct cgaactcgac ccccatgtga agctcggacc gagcaaaccg ctggaccttt
     2821 gcattcgccg gctcggctga gctgacggcg ggaaacagcg ccgccaacat ccaggctgcc
     2881 agcatgctcg tggcagcaaa cctgcgattt cgccggagtg attgcacctc gatctccctt
     2941 gacccgactg gtggcattgc ctaccgtccg ctcgccatgc cgcgccttca ttatgcgctc
     3001 gccgtgcccg tctttcttgc ctgcctgtcg gccggttggg gacagatagc gctgtcgcca
     3061 caggccgggc tgctggtgct tcgcaacggg caggtactgg aaggcaacgt aacgcgggcc
     3121 ggcgattact acattgtcac gctgggcggc acgagcgaaa tccgcctgcc ggccgccgac
     3181 gtggaagccg tctgcgggtc gctggacgaa gcctacgagt tcaaattgcg acacctctcg
     3241 ggcagcggcg cccgcccgca tttggagctg gcggaatggt gcctccggca acaattgcac
     3301 gcccgctgcg ccgagcagct cgtcagtgcc atgcgcctgg agccgcagaa tccgacgctc
     3361 aagctgctgg agcggcggct ggagtttgcc gccctgccgc cgaagaccga ccagccgaag
     3421 acagccgctg cgtcggagtt cgtcggcacc gagcaggtcg agaaaacgat ccgcgccctc
     3481 cccggcggca gcgtggagaa gttcgcggcc gtcgttcagc cgatcctgct gaaccgctgc
     3541 ggggccaacc agtgccacgg gccgaattcc aaggccgaat tccgcctctt gcggcccgct
     3601 cccgggcaac tggccagccg gcgctttacg gagcggaatc tctatgcggc cttgcagcag
     3661 ctcgattctt cgcatccaga tagcagcccg ctcgtcgcgc tgccgcagca acgacacgga
     3721 tcgtcactga ccgccgtctt cgacaagcac agccagcagc agctcgatga gctggtcgcc
     3781 tgggtcaagt tgacgctcgc cgcaccgcag tccaatcctg ccacgatcgc cgtcagctcg
     3841 cccacgctct cgcaagccgc cagcgcgacg gcaactcccc cagcaacgcc gaaagacaac
     3901 gctgccgccg ccgccgcagc ggctgtccgc gcgctaaaac cggccgccga ttccagccca
     3961 tccagtccgc ccgcagatcc gaatcacttc gttccacgcg accgcttcga tcccgagatc
     4021 ttcaatcggc ggtttcccaa atcttgagag cctgtcggca gcgacgcttc acttgcttcg
     4081 tgcgtctcct cgctcccacg cctcgagaaa ttccaggttg ctcgccagaa gctgcgg
//
LOCUS       DASUGB010000222         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1692504, whole genome shotgun sequence.
ACCESSION   DASUGB010000222 DASUGB010000000
VERSION     DASUGB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692504"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..754)
                     /locus_tag="VFV87_04350"
     CDS             complement(101..754)
                     /locus_tag="VFV87_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5103015.1"
                     /translation="MRRYTATARVYPTFEALLAAVRDCRACEAHLPLGPRPVLRASET
                     ARILVVGQAPGLRVHKTGIPWDDPSGERLRSWMGVDKTVFYDESRIAIIPMGYCYPGR
                     GSGGDLPPRRECASLWLDQLLARMPRIALTLLIGLHAQTHFLGSRRKRSLAETAKAWR
                     EYAPEFLPLPHPSPRNQPWLKRHSWFERELVPVLRLRINAIGNEPVAGAGDSSHPLP"
     gene            complement(807..2390)
                     /locus_tag="VFV87_04355"
     CDS             complement(807..2390)
                     /locus_tag="VFV87_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5103016.1"
                     /translation="MSEAVDILSGPDLTAGVPLTQVPDGAMLLGHAHGEPVLLVRRGD
                     ELSAVGAFCTHYGAPLVDGLLVDDTVRCPWHHACFSLRTGEALRAPALDPISCWRVEQ
                     RNGSAYVGEKLEIARQQSQPAAAGMPRSVVIVGGGAAGNAAAETLRREGYCGSITMLS
                     ADTSVPYDRPTLSKGYLAGMAPVESTPLRSQAFYAEHAITVKLGAPVATIDTTNRQVH
                     LADGTRHPYDVLLLATGAEPIRLDLPGSDLPHVHYLRTLADSRALVAKALTSQRAVVM
                     GASFIGLEVAASLRTRNIEVHVVAPDTVPMEKVLGTKVGQFIRKLHEQHGVTFHLGTV
                     AASIDDRGVTLQNGERLPADLVVIGVGVRPAISLAERAGLATDRGITVDEYLEASIPG
                     IFAAGDVARWRDRLTGERIRVEHWVVAQRQGQTAARNMLGRQERFDAVPFFWTEQYDF
                     GLAYVGHAERWDQAEIDGTLDTDTLDCTITYRRNGRKLAVAVVRRDLEGLRAEVEFER
                     AMRAHSTLDDPSELAITRR"
BASE COUNT          490 a          892 c          862 g          509 t
ORIGIN      
        1 gaatggggga attcgctcgt ggcatgatcc gcctcctctt gttttgtcgc ccggcgaggc
       61 cccttgtact cctgctttcg gtttcgagtc aacacgaacg tcatggcaag gggtgagagc
      121 tgtcgccagc tccggctacg ggctcatttc ctatcgcgtt gattcgcagg cgcagcaccg
      181 gtacgagctc ccgctcaaac caggaatgtc gcttgagcca cggctggtta cgcggcgagg
      241 ggtggggcag tggcaagaat tcgggagcgt actcccgcca cgccttggcc gtttcggcca
      301 gcgaccgctt gcgacggctg cccaggaagt gggtctgggc atgcaggccg atgagaagag
      361 tcagtgcgat ccgcggcatt cgggccagca attggtcgag ccacaggctc gcacattcac
      421 ggcgtggggg caggtcaccg ccactgcctc ttcccggata gcagtagccc atgggaatga
      481 tcgcaatacg tgactcgtcg taaaacacgg tcttgtccac gcccatccac gagcgcagtc
      541 gctccccgct cgggtcgtcc catggaatac cggtcttgtg cacgcgcaaa ccaggggcct
      601 gccccacgac gaggatgcgc gccgtttcac tcgcacgcag cacgggacgc gggccgagcg
      661 gcaaatgtgc ctcgcacgcc cgacagtcgc ggacggctgc cagcaatgct tcgaaggtag
      721 gataaacgcg ggctgtagcc gtgtatcgcc gcatcagtat ggtccgggga tcataggtcg
      781 cagagccctc tcagccgttc gccttctcag cgccgcgtga tcgccaactc tgaggggtca
      841 tcaagtgttg aatgggcccg catagccctc tcgaattcga cttcggcgcg caggccctcg
      901 agatcccggc gaacgacagc aactgccaac ttcctaccgt tgcgccgata ggtaatcgta
      961 caatctaggg tgtctgtatc gagggtcccg tcgatctcag cctggtccca gcgctcggca
     1021 tgaccgacat aggcgagacc gaaatcgtac tgctcggtcc agaagaacgg cacggcgtca
     1081 aacctctcct gccgcccgag catgttgcgt gccgcggttt gtccctggcg ctgggccacg
     1141 acccagtgct cgacacgtat gcgctcgccg gtgagccgat cccgccagcg ggcaacgtcg
     1201 ccggccgcaa aaatgcccgg gatgctcgcc tcgaggtact cgtccaccgt gatcccgcga
     1261 tcggtggcga ggcctgccct ctccgccagc gagatcgcgg gccggactcc taccccgatg
     1321 accacgagat cagccgggag acgctctccg ttctgcagag tgacaccacg gtcgtcgatc
     1381 gaggcggcaa ccgtgccgag gtgaaaggtc actccgtgct gctcgtggag cttgcggatg
     1441 aactgtccca ccttcgtccc caggaccttc tccatgggaa ccgtgtccgg cgcgacgacg
     1501 tgcacttcga tgttcctcgt ccgaagcgat gccgctacct cgagcccgat gaagctcgcg
     1561 cccattacca ctgcgcgttg cgaggtcagc gccttggcga ccagggcacg actgtcggca
     1621 agcgtgcgca ggtagtggac atgcgggaga tcgcttccgg gcagatcgag ccgtatcggt
     1681 tcggcgccgg tagcgagcag cagaacgtca tagggatggc gggtgccgtc cgcgagatgt
     1741 acctggcgat tcgtcgtgtc gatcgtagca accggagcac ccaacttcac ggtgatggcg
     1801 tgctccgcgt agaaggcctg tgatcggagg ggggtggact ccactggcgc catgccggca
     1861 agatagccct tcgaaagagt gggacggtca tatggcacgg aagtgtccgc gctcagcatc
     1921 gtgatgctac cgcagtaacc ctcccgccgg agcgtttccg cagctgcatt acccgctgcg
     1981 ccaccgccca caatcacgac cgatcgcggc atacccgctg ctgctggctg tgattgctgt
     2041 ctcgcgatct cgagcttctc tccgacatat gcgctgccgt tccgctgctc gacgcgccag
     2101 catgaaatgg ggtccagcgc cggggcgcgc agagcctcgc cggtgcggag actgaagcac
     2161 gcgtggtgcc aggggcaacg aactgtgtcg tccacgagca gcccgtccac gagcggcgcg
     2221 ccgtaatgcg tacagaatgc gccgactgcg gacagttcgt ccccacgccg caccagcaat
     2281 accggttccc cgtgagcgtg ccccagtagc atggcaccat cgggaacttg ggtgagcgga
     2341 actcccgcgg tcaggtcagg tccgctgagt atgtctaccg cctcgctcat ctcatctcca
     2401 tggcatcggt ctacggtcat gtcgcaagcg ccatgccgaa gatctctcga ccgaggaccg
     2461 actcttcagg gtggtcgggt tgttcgtcag tcgcgaagat tcgcgacgaa tgtcattgct
     2521 caggtagcga gagagccggg aggatcctcg ccggcggttc aattctcccc ggtccatgac
     2581 ggggcccata tctgaggtgt atgatcgcgc gaagtatggc cctggggcgg ggccgtgcga
     2641 tcttcacgtt cctagtcctc atggagactt ctgtggccga tactccccaa cacgagccgc
     2701 accgccggcc ccacgcaccg ccgatggcgg gtccattcat ggaattcgat ctc
//
LOCUS       DASUGB010000223         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1136222, whole genome shotgun sequence.
ACCESSION   DASUGB010000223 DASUGB010000000
VERSION     DASUGB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136222"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFV87_04360"
     CDS             <1..528
                     /locus_tag="VFV87_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5103017.1"
                     /translation="TWLIVLFLGEMLTASAMGLFEGEIAKAVVLALFVPLIISSGGNS
                     GSQAATLIVRALAVQEISLSDWWIVMRREIVSGLSLGLVLGTIGFARIALWTLFTPMY
                     GPHWVLVGLTVAISLVFIVMWGTLSGAMLPLVIKRCGLDPATSSAPFVATLVDVTGLV
                     IYFTVALFVLRGTLL"
     gene            990..2405
                     /locus_tag="VFV87_04365"
     CDS             990..2405
                     /locus_tag="VFV87_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5103018.1"
                     /translation="MIRTVVAGCALFSLCIGWRDSADGAELRAGFATAEITPAVEGAK
                     PVYLAGYGMGRTAEGVHDPLFARTVVLASGEKRVAIVSVDLVGLQYPQVKAIRAKLPH
                     LNYVLVSSTHNHEGPDVIGIWGRNPFHRGVDEEYLELVVERVVESIRAAEKSLVPVSA
                     AFGTAEDETLVGDSRQPFVKDGAIRVVRFQRPGSSDLAGLMVVWSCHPESLGSKNKLI
                     TADFPWATVAALQKRHKCPVVYLSGAVGGLMAPPDGRIHDSEGKEIKDGNFEYARRYG
                     EEVANLADKALAAAEPIELTPFAISAKAIAVPVENSYYKAARALGVLQRDGIPWTGNF
                     DDVGPPQPPDAGERLAAVESEVAYLRLGDLHVAGVPGEIYPELVYGKFQEPADPGADF
                     PDAPLEPSVAALLSGKRWLLIGLANDELGYIIPKRQWDAAAPFAYGRTKSQYGEINSC
                     GPDVAPILMRALQARIDSAHK"
     gene            2633..3358
                     /locus_tag="VFV87_04370"
     CDS             2633..3358
                     /locus_tag="VFV87_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5103019.1"
                     /translation="MIEAVGLSKYYGVFAACRNVSFEIKKGEIVAFLGPNGAGKSTTM
                     KLLTGYLAPSEGVARIAGHDMAADRLAGSARLGYLPENGPLYPDMTPHSILNFFAEAR
                     GMEGDYKHQRIDAVIELCMLGSVIHRPISKLSKGFRQRVGMAQALLHEPDVLIMDEPT
                     AGLDPNQIVEVRKTLRRIGEEKTILLSTHILQEVDAMAQRVILINEGSLVYDGPARDL
                     TNKYGSLDGAFHSLTAVAAAVED"
     gene            3485..>4109
                     /locus_tag="VFV87_04375"
     CDS             3485..>4109
                     /locus_tag="VFV87_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5103020.1"
                     /translation="MTAVILEMLLLLAIDAAFVIVAGLLLVLLAMTKKAAFAVLKRNF
                     VGYFSNPTGYVFLCLFVLLTSAAAFWPHDFFVANLANLDQLNKQITLILLVFIPAITM
                     SIWAEERRQGTDELLLTLPAADFDIVIGKYLAAAAIFSVSLIFSQLANYFVLVSLAWD
                     GQSNFVDIDTGLFFTTYLGYWMMGLAMLSIGMVASFLTNNLTVSFILA"
BASE COUNT          738 a         1250 c         1290 g          831 t
ORIGIN      
        1 acatggctga tcgttctgtt tttgggcgaa atgctcacag catcggcgat gggcttattt
       61 gagggcgaaa tcgcaaaggc ggttgtgctg gcactcttcg tgccgctgat catttctagc
      121 ggcggcaatt ccggttccca ggcggccacg ctcattgtgc gggcgctcgc cgttcaagag
      181 atttcgctca gtgactggtg gatcgtgatg cgccgggaga ttgtctccgg actctctctg
      241 ggcctcgtgc tggggacgat tggttttgct cggatcgcgc tctggacgtt gttcaccccg
      301 atgtatggac cgcactgggt gctagtcggc ctgaccgtgg ccatttcgct cgtgttcatc
      361 gtgatgtggg gaacgctttc cggcgcaatg ctgcccttgg taatcaaacg ctgtggcctg
      421 gaccccgcca ccagctccgc gccgtttgtc gccacgctcg tcgatgtgac cgggctggta
      481 atctatttca cggtcgccct cttcgtcttg cggggaacat tgctctagtc cgagcggacg
      541 ccttcgcctc tgaaggcgcg caaaaaaaag cccccgaaac atcgaatccc aggggggcta
      601 gagattcgcg gtgttccggg agcgattgga cggttgggcg tcgcgtccct gcgcgcctgg
      661 ccgtacacgg ccgatcggct tgactgcccc tcgcttccgc atccctgcgg gtacgagcag
      721 gcagcgtccc gcagaacgcc acgggcacgt tccaggtgcg ggatccgcca cgcggccgct
      781 gttgttcgaa ccgtgtccaa cggcaccagc ggcgaggcat ccgtgccagg ccctcgtgcg
      841 tcgaacgagg cgggaagata ggccattgcc caaaacgggt caatgctgaa tttcgggccc
      901 gcttggcagc gatttttcgc gaacctggca agtcgtccga gcccagccgc tataatcagc
      961 cgctcgccag ggaacttggg atcgaaatca tgattcgaac ggttgttgcc ggatgtgcgc
     1021 tgttcagtct gtgcatcggg tggcgggatt cggccgatgg ggcggaactc cgggccggat
     1081 tcgccacggc ggagatcacg cccgcggtcg agggcgccaa gccggtctat ctcgccggct
     1141 atggcatggg ccgaacggcg gagggcgttc acgatccgct gtttgcccgc accgtggtgc
     1201 tggccagcgg cgaaaagcga gtggcgatcg tttcggtgga tctcgtcggg ctgcaatacc
     1261 cgcaggtgaa ggctatccgt gcgaagctgc cgcacctgaa ctacgtcctc gtcagcagca
     1321 cgcacaatca cgaggggccg gatgtgatcg gcatttgggg ccgcaatccg tttcaccgcg
     1381 gcgtggatga ggaatatctg gaactcgtcg ttgagcgcgt cgttgaatca attcgcgcgg
     1441 ctgagaagag cctggtcccc gtctctgccg ctttcggcac ggccgaagac gaaacgctcg
     1501 tgggcgacag cagacagccg ttcgtgaagg atggtgcgat ccgcgtggtg cgatttcagc
     1561 ggcccggtag cagcgatctc gccggtctaa tggttgtctg gagctgccat ccggaatcgc
     1621 tcggctcgaa gaacaagctc attacggccg attttccgtg ggccaccgtg gcggcgctcc
     1681 agaagcggca caagtgcccg gtcgtctatc tcagcggcgc ggtcggcggc ctgatggcgc
     1741 cgcccgacgg ccgcattcac gattccgaag gcaaagaaat caaggacggg aatttcgagt
     1801 acgcgcgccg ctacggcgag gaggtcgcca atctggccga caaagccctc gcggctgccg
     1861 agcccatcga gttgacgccc tttgccattt cggcgaaagc catcgcggtt ccggtcgaga
     1921 actcgtatta caaggccgct cgggcactgg gcgtacttca gcgggatgga attccctgga
     1981 caggcaactt tgacgacgtc ggcccgccac agccaccaga tgcgggcgag aggctcgccg
     2041 ccgtggaaag cgaggtcgcc tatctgcggc tgggcgacct gcacgtcgct ggcgtcccag
     2101 gcgagatcta tccggaattg gtttatggaa agttccagga gccggccgat cccggcgccg
     2161 attttcccga tgctccgctc gagccttcgg tcgccgcgct gctttccggc aagcgctggc
     2221 tgctgatcgg cctggcgaac gacgaattgg gctacatcat tcccaagcgg cagtgggacg
     2281 ccgccgcgcc cttcgcttac ggccgcacga aatcgcaata tggcgaaatc aatagctgcg
     2341 gtccggacgt cgcacccatc ctgatgcgtg ccctccaggc gcggatcgat tccgcccaca
     2401 aatgagtacc cgaaccatcg attcgctgcc ggcgtaactt atcgtatgag ttgacgggtc
     2461 cggttgagcg ggcgacggaa accgttatat tgtcgggtgt tatgaatagg ccgttggccg
     2521 aaggccacga ccgactgacg atctatccag cagtcgattt tgtaatatga atgtggccca
     2581 cgaaggtcct gccgctaagg cggacaagaa agagcacgcc aacggccagc cgatgattga
     2641 ggcggttggc ctgtcgaagt actacggcgt ctttgctgcg tgccgcaatg tgtcgttcga
     2701 gatcaagaag ggcgaaattg tcgcctttct cggtcccaac ggcgccggca agagcacgac
     2761 gatgaagctg ctcacgggct atctcgcgcc gtcggagggc gtcgcgcgga tcgccggcca
     2821 cgacatggcc gctgaccgcc tggccggctc ggcccggctg ggatatctgc cggagaacgg
     2881 cccgctctac ccggacatga cgccgcattc gattctcaac ttcttcgccg aagcccgcgg
     2941 catggagggg gactacaagc accagcgaat cgacgcggta atcgagctgt gcatgctcgg
     3001 cagcgtgatc catcgcccga tcagcaagct gtcgaaggga tttcggcagc gtgtcggcat
     3061 ggcccaggcc ctgttgcacg agccggacgt gctgatcatg gacgagccga cggctggcct
     3121 ggacccgaac cagatcgtgg aggtccgcaa gacgctgcgg cggatcggcg aggagaagac
     3181 gattctcctt tcgacgcaca tcctgcaaga ggtcgatgca atggcccagc gggtgatcct
     3241 cattaatgaa ggctcgctcg tctatgacgg gccggcccgc gacctgacca acaaatacgg
     3301 cagcctggac ggtgcgtttc actccctgac ggccgtcgcc gccgccgtcg aggactagcc
     3361 gtagccgcaa cgtccgtagg accggtttcc aacccgtccg agcggacagg ttgaaaacct
     3421 gtcctgcagt acgagggccc acgaaaggtt gatgattctc tcccaaaaac cggattgaga
     3481 tgcgatgact gcagtgattc tggaaatgct cctcctgctg gcgattgacg ctgccttcgt
     3541 catcgtggcg ggcctgttgc tcgtgctgct ggcgatgacc aagaaggccg ccttcgcggt
     3601 cctcaagcga aacttcgtcg gctatttcag caatccaacc ggctatgtct tcctgtgcct
     3661 gttcgtgctg ctgacctcgg cagcggcgtt ctggccgcac gatttcttcg tggcgaatct
     3721 ggccaacctc gatcagctca acaagcagat cacgctgatc ctgttggtct tcattcccgc
     3781 gatcacgatg agcatctggg ccgaagagcg acggcaaggg accgacgagc tcctgctcac
     3841 gctccccgcc gccgacttcg acatcgtcat cggcaagtac ctggcggccg cggcgatttt
     3901 ctctgtctcg ctgatattct cgcaattggc gaactacttc gtcctcgttt cattggcgtg
     3961 ggacggccaa tcgaacttcg tcgatatcga cacgggtctc tttttcacga cctatctcgg
     4021 ctactggatg atgggcctgg cgatgctctc gatcggcatg gtggcgtcgt ttctgacgaa
     4081 caacctgacc gtcagcttca ttctggccc
//
LOCUS       DASUGB010000224         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_25277,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000224 DASUGB010000000
VERSION     DASUGB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25277"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1733
                     /gene="leuC"
                     /locus_tag="VFV87_04380"
     CDS             315..1733
                     /gene="leuC"
                     /locus_tag="VFV87_04380"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5103021.1"
                     /translation="MSATPRTMFQKIWDSHIVHAEPGKQTILYIDLQLVHEVTSPQAF
                     EGLRLSGRKVRRPERTVATPDHNIPTTDRSLPIADPISRQQVETLRANCREFGIKLYD
                     MNDIRQGIVHVIGPELGITQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQT
                     LLQQVPKTLELRVSGPLGRGVTAKDIILYLIGQITTDGGTGYVIEYTGAGVQGLNMEE
                     RMTVCNMSIEAGARAGMIAPDQTTFDYIKGREFAPQGADFDKAVARWKELPTDAGAKY
                     DKSLVFQAKDISPQVTWGTNPGQVAPVIGKVPGPADFTDPTDQKTTERALEYMGLAAG
                     TPLTDVKIDRVFIGSCTNARIGDLRAAAAVVKGHKIAGSVHAMVVPGSGQVKLQAEKE
                     GLDRIFKEAGFEWREAGCSMCLAMNPDKLEPGERCASTSNRNFEGRQGKGGRTHLVSP
                     AMAAAAAVAGHFVDVREWNYKA"
     gene            1774..2091
                     /locus_tag="VFV87_04385"
     CDS             1774..2091
                     /locus_tag="VFV87_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103022.1"
                     /translation="MTKAQRKMLTAYKAFRDSPPTVLTLLPRFLRPWLLLTVVCGVLV
                     ALGVVNGFLFVPRLAVGLLVGCVARDIGWSLKTVSGWPTTREVINWDRVEQLLSQDPT
                     NPN"
     gene            2130..>2236
                     /locus_tag="VFV87_04390"
     CDS             2130..>2236
                     /locus_tag="VFV87_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5103023.1"
                     /translation="MQPFTTHTGLVAPMDRANVDTDQIIPKQFLKRIERT"
BASE COUNT          461 a          686 c          708 g          381 t
ORIGIN      
        1 ggactattca cggcctcaag aaatgaaaca gcttccaggc gcgtgtacga agtggaatag
       61 caacgacttt gaagggcgcg gcgagtcgtc gaatgagatc gacttgagtt cgagagcctt
      121 ctaaaaacca gttacttgca gtgattgcga ctctgttttc gagtgcttcg gagtggcatg
      181 ctgcgggcac taggccccgt acgccgcctg ccggagcaag tgcttttggc ctataataca
      241 gagtttggct gccgccgctc gtttgcagca cgctgcaccc aagtttcgcc tccgccagca
      301 ttcgcaattc ccccatgtcc gctacgcccc gcacgatgtt ccagaaaatc tgggacagcc
      361 atatcgttca tgccgagccc ggcaagcaaa cgatcctgta catcgacctg cagcttgtgc
      421 acgaggtgac cagcccgcag gcgtttgagg ggctgcggct gagtgggcgg aaggtgcggc
      481 ggccggagcg gacggtggcg acgccggacc acaacattcc gacgaccgac cgcagcctgc
      541 cgatcgccga tccgatcagc cgccagcagg tcgagacgct gcgagccaac tgccgcgagt
      601 tcggcatcaa gctctacgac atgaacgaca tccggcaggg gatcgtccac gtcatcgggc
      661 cggagctggg catcacgcag ccgggaatga cgatcgtctg cggcgacagc cacacggcga
      721 cgcacggggc gttcggggcc ctggcgttcg gcatcggcac gagcgaggtc gagcacgtgc
      781 tggcgacgca aacgctgctg caacaagtgc cgaaaacgct ggagctgcgc gtcagcggcc
      841 cgctcggccg cggcgtgacg gcaaaggaca tcatcctgta cctgatcggc cagatcacga
      901 ccgacggcgg cacgggctac gtcatcgaat acacgggcgc tggcgtccaa ggcctgaaca
      961 tggaagagcg gatgacggtc tgcaacatgt cgatcgaagc cggcgcgcgg gccggcatga
     1021 tcgcgcccga ccagacgacc tttgattaca tcaaaggccg cgagttcgcg ccgcagggcg
     1081 ccgatttcga caaggccgtc gcccgctgga aggagctacc gaccgacgcc ggcgcgaagt
     1141 acgacaagtc gctcgtcttc caggcgaagg acatttcgcc gcaagtcacc tggggcacga
     1201 accccggcca ggtcgcgccg gtgatcggca aagtgcccgg tcccgccgat ttcaccgatc
     1261 cgacggacca aaagacgacc gagcgggcac tcgaatacat gggtctggca gccggcacgc
     1321 cgctaaccga tgtgaaaatc gaccgcgtct tcatcggcag ttgcaccaat gcccggatcg
     1381 gcgatttgcg ggcggcggct gcggtggtga aggggcacaa gattgccgga agcgttcacg
     1441 cgatggtcgt tcctggcagc gggcaggtga agctgcaggc cgaaaaagaa ggactcgacc
     1501 gtatcttcaa ggaagccggc ttcgagtggc gcgaggcggg ctgctcgatg tgcctggcga
     1561 tgaaccccga caagctggag ccgggcgagc gctgcgccag cacgagcaac cgcaatttcg
     1621 aaggccgcca aggcaaaggc ggccgcaccc acctcgtctc ccccgcgatg gccgccgccg
     1681 ccgcggtcgc ggggcatttt gtggatgtga gggagtggaa ctacaaggcg tgaccgccgg
     1741 tcgtcatttc caaaagcgaa gggaccaatg ggtatgacga aggcgcagcg caagatgttg
     1801 acggcctaca aggcgttccg cgattcgccc cccaccgtct taacgttact gccgcgtttt
     1861 ctgcggcctt ggttgctcct gactgttgtt tgtggggtgc tggtcgcact tggcgttgtg
     1921 aacgggtttc tgtttgtgcc caggctcgcc gtaggcttgc tcgttggctg tgtcgcaagg
     1981 gatatcggat ggtcgctcaa gacggtaagc ggctggccga cgaccagaga agtcatcaac
     2041 tgggatcgcg tcgagcaact tctcagtcag gatccgacca accccaactg atccactaca
     2101 ccaactttat cattcgaaat aagagcccta tgcaaccctt caccacacac accggtctcg
     2161 tcgccccgat ggaccgggcg aatgtcgata cggaccagat cattcccaag cagttcctca
     2221 agcggatcga gcggac
//
LOCUS       DASUGB010000225         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_163842, whole genome shotgun sequence.
ACCESSION   DASUGB010000225 DASUGB010000000
VERSION     DASUGB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163842"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..1972
                     /locus_tag="VFV87_04395"
     CDS             503..1972
                     /locus_tag="VFV87_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103024.1"
                     /translation="MSYRHWLMLVACLLPGAIGAPDLAAQTLNLEPLPPIDAVLQPVG
                     YEDYFDVGSVVEPGAIAPLPAPPGGPPPTAAMTPPACPPQDEACPHCPCADCCCWPCE
                     CPCPPAACLPCPRINLINPCWQLLVGGNITLDMLFNSSRPVAPGTPFFLTPSGPFSDS
                     TFDMHARQTTMYFALMGPQMGNFRSGGLILFNLYNDSVSADRYGFLPYQAYGELKNEY
                     WRFAGGLQSDIFAPLLPTVLPFSYLMASGNTGVFRGQLRAERFFYPAFAEQITFTAGI
                     SEPISTVYNNSTLVTGSTALTEDNGIPDLEMRLAWAVGPESQVGLETRRPFEVGISTV
                     LGQIRTTFAPPLTRVVDDVWGVAADFRWRVNDLWGFSGELFHGQTLGTYGGGVFQNVN
                     SVTFTPVHATGGWLQFDWYYTPCLHSHFGFGVDDPANSELALGQIGLNQTVFANLIWD
                     LNTSFRIAGELTFRETDYVGPAALSNDGVGIQGQMQWKF"
     gene            complement(2073..2951)
                     /locus_tag="VFV87_04400"
     CDS             complement(2073..2951)
                     /locus_tag="VFV87_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX5103025.1"
                     /translation="MRWQGGRQSDNLEDRRRMGRGGGIAVGGGVVLIIALVLLCLGAD
                     PRQVLQLVQNAPVQQGGGAGPGAEQPETPEEAHTREFAATILAFTEEVWGEQFRRAGH
                     VYEPPKMVLFSERVLTGCGAAPSSVGPFYCPADKTVYLDPTFFDELEERLGGSKAEFS
                     QAYVIAHEIGHHVQNLLGYSDLVHGKQRALPDEEGNRWSVRLELQADYLAGVWAHYGQ
                     QKFHFIEPGDIEAAIQSANAIGDDRLQRNAGGFVSPESFTHGTSAQRTKWFALGLETG
                     DLSRIEELFELPYERL"
BASE COUNT          564 a         1058 c          965 g          652 t
ORIGIN      
        1 caggttcgcc aattcaggcg atttgccggt gttaaaagcc gtcaccaact tcccggccgc
       61 ggcccgaatc tcgggggcga tcttctcgtc ctgggcggag actgcagaaa ctgccagaca
      121 gagcatggcg gtgggaaccg caacggagcg aaacagcgca gacatgacag cacctgcatg
      181 aagtgtgaga aaggcagcca acccgcggaa ccggaccatc ccacgagcac ccgaaaagag
      241 aggaattggc ttggcgacga gtccaggcaa ttcgcccggc cagccatcct tgcagcggca
      301 attatggcac tcccgcaacc ggttgcaaag cgggcgactt tgcggcttgg gagggtctta
      361 gcggcctggc taacgcggag cgtgccgttt gtgcgttttt ttccaggcgt gacgcggttg
      421 cggacgatac cgatcatcag cgttgttccg cttatgacgg taatttcgcc agatcttcac
      481 acgggcagct tggtgccgag acatgtcgta tcgccattgg ttgatgttgg ttgcctgcct
      541 gctgccaggc gcaattggag ccccagacct tgcggcgcag acgctgaatc tggaaccgct
      601 gccgccaatc gatgctgtcc tgcaacctgt cggctatgag gactattttg acgtcggctc
      661 ggtcgtggaa cctggcgcaa tagcgcctct gcccgcgccg ccaggcggcc cgccgccgac
      721 cgctgcgatg acaccgcccg cctgcccgcc gcaggacgag gcatgcccgc attgcccctg
      781 cgccgattgc tgctgctggc cgtgcgaatg tccctgcccg cccgcggcgt gcttgccctg
      841 cccgcgcatc aatctgatca acccttgttg gcagctcttg gtcggcggca atatcaccct
      901 cgacatgctc ttcaatagca gccggccagt cgccccgggc acgccttttt ttctcacgcc
      961 cagcggcccg ttcagcgaca gcaccttcga catgcacgcc cgccagacga cgatgtactt
     1021 tgcactaatg ggcccgcaga tgggcaactt ccgctccggc gggttgatct tgttcaacct
     1081 ttataacgat tcggtgagtg ccgaccggta cggcttcttg ccctatcagg cctatggtga
     1141 attgaagaat gagtactggc gctttgccgg tggtttgcaa tcggatatct tcgccccgct
     1201 cttgccgact gtgctcccct tcagctattt gatggcgtcg ggcaacacgg gcgtcttccg
     1261 cgggcagctc cgggccgagc gatttttcta tccggcattc gccgagcaga tcacgttcac
     1321 cgccggcatc agcgagccga tctccaccgt ctataacaac agcacgctcg tcaccggttc
     1381 cacagccctg accgaggaca acggcattcc cgatctggag atgcgcctgg cctgggctgt
     1441 gggacccgag tcgcaagtcg gcctggagac caggcggccg ttcgaagtcg gcatttcgac
     1501 cgtgctcggg cagattcgga cgaccttcgc accgccgctg acgcgcgtcg tcgacgacgt
     1561 gtggggcgtg gccgccgact tccgctggcg cgtgaacgat ctatgggggt tctcgggcga
     1621 attgttccac ggccaaacgc tgggcaccta cggcggcggc gtattccaga atgtgaattc
     1681 ggtgactttt acccccgtgc atgccaccgg ggggtggttg cagttcgatt ggtactacac
     1741 tccctgcctg cactcgcact tcggctttgg cgtcgatgac ccggccaaca gcgagctggc
     1801 tctcgggcag atcgggctca atcagactgt gttcgccaat ctgatctggg acctgaatac
     1861 cagtttccgc attgccggcg aactcacctt ccgcgaaacg gactacgtcg gccccgccgc
     1921 tctttccaac gacggcgtcg gcatccaggg ccagatgcag tggaagtttt agtagaccgt
     1981 tcggactcgt agccgcactc gccagagtgc gggcaggttg gcccgcgctc ccgcgttctg
     2041 gcgaacgcgg ctacgaaggc tcgcgtccgt gatcacagcc gctcgtacgg caattcaaac
     2101 agctcttcga tccggctgag gtcgccggtt tccaggccca gggcgaacca tttcgttcgc
     2161 tgggccgagg tgccgtgcgt gaagctctcg ggcgagacga agccgccggc attccgctgc
     2221 aggcggtcgt cgccgatggc gttagccgat tgaatcgccg cttcgatgtc gcccggctcg
     2281 atgaaatgaa acttctgctg cccgtagtgc gcccacacgc ctgccagata atcggcctgc
     2341 agttccagcc gcaccgacca ccggttccct tcctcgtcag gcaaagcccg ctgcttgccg
     2401 tggaccagat cgctatagcc cagcaggttc tgcacgtggt ggccgatctc gtgagcaatc
     2461 acatacgcct gcgagaactc ggccttggag cctcccagcc gctcttccag ttcgtcgaaa
     2521 aacgtcgggt cgaggtacac cgttttgtcg gccgggcaat agaacggccc gaccgacgac
     2581 ggcgcggctc cgcagcccgt cagcacgcgc tcggagaaga ggaccatctt cggcggttca
     2641 tacacgtggc cggctcgccg gaattgctcg ccccacactt cttcggtgaa ggccagaatc
     2701 gtggccgcaa actcgcgggt gtgggcctct tcgggagtct caggctgctc ggcacccggc
     2761 ccggctccgc cgccttgctg cacgggcgca ttctgcacga gttgcagcac ttgccgcgga
     2821 tcggcgccca ggcagagcaa aacgagcgcg atgatgagga ccacgccgcc ccccacggca
     2881 attcccccgc cacgccccat gcgccggcga tcttccaggt tgtcactctg tctgccacct
     2941 tgccagcgca tgagattgac tccgtgattt acactttgca gccaccaacc cggcgccgct
     3001 ggaagaattg tcccgcgtga ggattcgaag ggctgtctgt tcaattccga atgcgtgcga
     3061 acagcaactt atgcgccgga tttgagctat gggtcaaacg ggtagattcg cttccaatcg
     3121 ttcttcatat ccaccaccgt ccagccgcga tcgtttgcct ccgctagagc cttctccagt
     3181 tgtccgacga ctgagggatg gtcgtaggcc cattcgcgct tggcgtcggt gtggtggac
//
LOCUS       DASUGB010000226         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_25415,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000226 DASUGB010000000
VERSION     DASUGB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25415"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFV87_04405"
     CDS             complement(<1..1539)
                     /locus_tag="VFV87_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5103026.1"
                     /translation="MSKISFPRCTEVILLALAAAIWQPSAGAQNQPPERSQPPAAPSL
                     LVGLAAADLTPPDGFPMAGYYHERLAEGTIDPLQAKAIVLRSGKTEAALVVCDLIGIA
                     TDLSREVRRRAAEQTGIPADHIVLSATHSHTAPDYMKELYLNLGGQRQEPARAAYIGK
                     LLSGPAEAIVKARAAAQPVILEAGSATLQPQVSFNRRFVMRDGSVRTWMNLDHPEVVR
                     SAGPIDPNIELLSIKSAAGKPLGILSNFALHLDTVGGSKWSADYPHFVQESLRNATGS
                     DVLSIFATGCCGDINHADPRRRERNKTDFIGNAIGASIAQQLDHLEPVGQPNLVVKSR
                     LVPLPLQDATADEVARAIQTLDAAARQEKVDFYEHVTAYKKLILDQLQHAQPYTNTAE
                     HITWGLSRSLAGVGPSLPAEVTVLALGRDMAIVCLPGEVFVELGLAIKQGSPFRTTLV
                     VELSNCVETIYIPTRASYAGGSYEVTNSALQPGAGEMLVEAALALLREAAEQNAPMPS
                     QPAKN"
     gene            1720..2979
                     /locus_tag="VFV87_04410"
     CDS             1720..2979
                     /locus_tag="VFV87_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5103027.1"
                     /translation="MKPSIAFALLLVLSSSTLPAADWPAWRGPTGQGFCEEEDVLLTW
                     SDTENVKWKVPLEHQGNSTPVIWGDKIFLTQANTDGEIRSLLCFARADGKLLWQQDVP
                     YGEKERNWNPNWYCNASPATDGERVVVSFASAGMYCYDFEGQELWKRTDLGGTWEHAF
                     GSGASPVLYGDLAILWVGPNEKGRNYLLAVNKSNGETVWEADQTYGSWSTPLITSVNG
                     QDQLLLGYSKDVKNAPPDKTGFLYGVDPKDGKELWRCQGLNSYVYTSPLYRDGVAVAM
                     SGYGGSAIAVKLGGTGDITADRLWEHPKNTQRVGSGMIVGDHIYMMDENGVPKCYELK
                     SGEEVWKVEKRPGGGNTWGSMVHADGRLYVLMRDGSTLVFAASPKYELLAANSLGRGV
                     STNSSLAISNGDIFIRTFGHLWCIGRP"
     gene            3201..4391
                     /locus_tag="VFV87_04415"
     CDS             3201..4391
                     /locus_tag="VFV87_04415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5103028.1"
                     /translation="MRACGDPPISVVLPDGQELEPAGGESLGRLRIADRKTLWRLAAN
                     PLYQFGEAYADGRLEVEGDLGEVLTMIFRRWNAAASGKPVIRPWLRLRWLRKNSPARS
                     RDNIHHHYDIGNDFYELWLDQQLVYTCAYFPTPEVTLEAAQIAKLDHVCKKLRLEPGE
                     HVVEAGCGWGALALHMARHYGVKVTAFNISREQVAYARQRAEREGLSGRVEFVLDDWR
                     SIRQPCDAFVSVGMLEHVGLSNYQQLGAAIGRCLTPSGRGLIHSIGQNQPMPMNPWIE
                     RRIFPGAYPPTLRQCMDVLEPHSFTVLDVENLRLHYALTLRHWLARFELASETVKQRF
                     GEKFVRTWWMYLAGSMAAFESGTLQLFQITFARGDCNRVPWTRAELYAQPGASPPGQW
                     AGNK"
     gene            4510..>5074
                     /locus_tag="VFV87_04420"
     CDS             4510..>5074
                     /locus_tag="VFV87_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103029.1"
                     /translation="MRIAVLAAASVAGLILTTSVLAQVRIRAEPAAIPAEAKPADPNK
                     PAEPAAEEPAIDVPRKPPVELGPKHIRLHLLDGSVISGDLSVAEITVDTPFGSLVVPI
                     DRIRSLRPGLDSYPKVLAEFEGLIQNLGSDDYQTREQAHKDLAAMGPKVRKELERFSS
                     DENAEIKRHVGEILKELEEQGQEAEEFD"
BASE COUNT          942 a         1558 c         1641 g          933 t
ORIGIN      
        1 gtttttcgcc ggttgactgg gcattggcgc gttctgctct gccgcttcgc ggagcaatgc
       61 gagcgcggct tccacgagca tctcgccagc gcccggctgc agggcggaat tcgtgacttc
      121 atagcttccg cccgcataag acgccctggt cgggatgtaa atcgtttcga cgcagttgga
      181 gagttcgacg actagcgtcg tgcggaaggg agagccttgt ttgatcgcca gccccagctc
      241 gacaaagacc tcgccgggca ggcagacaat ggccatatcc cggcccaggg ccaggacggt
      301 cacttccgcc ggcagcgacg ggcccacgcc cgccagcgag cggcttagcc cccaggtgat
      361 gtgctcggcg gtgttcgtgt agggctgcgc gtgctgcaac tgatcgagaa tgagcttttt
      421 gtaggccgtc acgtgctcgt agaaatcgac cttttcctgc ctggccgcgg cgtccagggt
      481 ttgaatcgcg cgagcaacct cgtccgccgt tgcatcttgc agcggcagcg gcacaaggcg
      541 ggattttacg actagatttg gttgcccaac gggctccagg tgatcgagct gctgggcaat
      601 cgaagcgccg atggcattgc cgatgaagtc ggtcttgttc cgctcgcggc ggcggggatc
      661 ggcgtggttg atgtccccgc agcagccggt ggcaaagatc gagagcacgt ccgagccggt
      721 cgcgttgcgc agcgattcct ggacgaagtg cggatagtcg gcactccact tcgatccgcc
      781 gaccgtgtcc aggtgcaggg caaagttgct gagaatcccc agcggcttgc cggccgcgct
      841 cttgatggac aggagctcga tgtttggatc gatcggaccg gccgagcgga ctacctcggg
      901 atgatcgagg ttcatccacg tccgcacgct cccgtcccgc atcacaaagc ggcgattaaa
      961 cgacacctgt ggctgcaggg ttgcggaacc cgcctccaga atcaccggct gggccgctgc
     1021 gcgggctttg acgatcgcct cggcgggccc gctgagcagc tttccgatgt acgcggcccg
     1081 agccggttcc tgccgctgac cgcccaggtt caggtacagc tccttcatgt agtccggcgc
     1141 ggtgtgcgag tgcgtcgccg aaagaacaat gtgatccgca ggaatgcccg tctgttcggc
     1201 agcccgccgg cgaacctcgc gagacaaatc ggtggcaatg ccgatcaggt cacagacgac
     1261 cagcgccgcc tccgtcttcc cgctgcgcag cacgatcgcc ttggcctgga gcgggtcgat
     1321 cgtcccctcg gccaaccgct cgtggtaata gccggccatc gggaatccat caggcggcgt
     1381 caggtccgca gcggcaagtc ccacgaggag cgatggcgcc gctggcggct ggctgcgttc
     1441 gggtggctga ttctgggcac cagcgcttgg ctgccaaatc gcggcagcga gagccaagag
     1501 aatcacttca gtgcagcgcg ggaaggagat tttggacacg cggtggcccc cgtaatcaat
     1561 ggtttcgctc gtcagcaagt cttcggtaca cgtgagctaa ttctagtccg ccgccagccg
     1621 acctcgcagg atgcccagtc aaggttgaat ggcgattggc ccgcgcgata cgatggcgtg
     1681 gcccaccact ccatcccata accccacccg agaactgccg tgaagccttc cattgccttc
     1741 gccctcctgc ttgtcctttc ttcctccaca ctgcccgcgg ccgactggcc cgcctggcgt
     1801 ggccccaccg gtcaaggctt ttgcgaagag gaagacgtac tcctcacgtg gagcgatacg
     1861 gagaacgtca agtggaaagt cccactcgag caccagggga attccacgcc ggtgatctgg
     1921 ggcgacaaga ttttcctgac gcaagccaac accgatggcg agatccgcag cctgctatgc
     1981 ttcgcccgcg ctgacggcaa gctgctctgg cagcaggacg taccctatgg cgaaaaggaa
     2041 cgcaattgga acccgaactg gtactgcaac gcctcgcccg ccaccgacgg cgagcgggtc
     2101 gttgtgagct tcgcctcggc cggcatgtat tgctacgact tcgagggcca agagctctgg
     2161 aagcggaccg acctcggcgg cacgtgggag catgccttcg gcagcggggc gtcgcccgtg
     2221 ctgtacggcg acctggcgat cctctgggtc gggccgaacg agaaaggccg aaactatctc
     2281 ctcgcagtca acaagtcgaa cggcgaaacg gtctgggagg cggaccaaac ctatggctcg
     2341 tggagcacgc cgctgattac ctcggtcaac ggacaggacc agctcctcct gggctattcg
     2401 aaggacgtca agaacgctcc cccggacaag accggctttt tgtatggcgt tgatcccaag
     2461 gacggcaagg aactgtggcg ctgccagggc ctgaacagct acgtctatac ctcgcccctg
     2521 tatcgcgacg gcgtggcggt ggccatgtcc ggctacggcg gctcggccat cgccgtcaag
     2581 ctcggcggca cgggcgacat taccgccgac cgcctctggg agcatcccaa gaacacgcag
     2641 cgcgttggct cgggaatgat cgtcggcgac cacatctata tgatggacga gaacggcgtc
     2701 ccgaaatgct atgagctgaa atcgggcgag gaagtctgga aggtcgagaa acgccccggc
     2761 ggcgggaaca cctggggttc gatggtccac gccgacggcc ggctgtacgt cctgatgcgc
     2821 gacggcagca cgctggtctt tgccgccagc cccaaatacg agctgctcgc tgccaattcc
     2881 ctcgggcgag gcgtatcgac caactcctcg ctcgccattt ccaacggcga catcttcatc
     2941 cgcacgttcg gccacttgtg gtgcatcggc aggccgtaga gtagccgttc gctctctcgt
     3001 gttgttgaat tgcacggatg ggcgcgcgat ggcgtataat cggtgactcg tgggggcacg
     3061 ctatgactcg tattgctgcg cgtaacggaa acagttcgga aggcgcacag gtccggcagc
     3121 gggaggcgca gggttcgcct cgtcctccgc ggtccggacg atcggcggag agttgggagg
     3181 cacgggcact aacccgacta ttgcgggcct gcggcgatcc gccgatcagc gtggtgctgc
     3241 cggatgggca ggaactcgag ccggccggcg gggaatcgct cggtcgcctg cggattgccg
     3301 atcgcaagac gctctggagg ctggctgcaa atcccctcta tcagtttggc gaggcctatg
     3361 ccgacggccg gctggaagtt gaaggcgatc taggcgaagt gctgacgatg atcttccgcc
     3421 gctggaacgc agccgccagc ggcaagccgg tgatccggcc gtggctgcga ttgcggtggc
     3481 tgcggaagaa ttcgccggcc cgttctcgcg acaacatcca tcaccattac gacatcggca
     3541 acgactttta cgagctgtgg ctcgaccaac agctcgtcta tacgtgtgcc tatttcccga
     3601 cgcccgaagt cacgctcgaa gcggctcaga tcgccaagct cgaccacgtc tgcaagaagc
     3661 tccgcctgga gccgggcgag cacgttgtcg aggcgggctg cggctgggga gcgctggcct
     3721 tgcacatggc aaggcactac ggcgtgaagg tcacggcgtt caatatctcg cgcgagcagg
     3781 tggcgtatgc ccgtcagcgg gccgagcgcg aaggcctgag cgggcgagtc gaattcgtgc
     3841 tcgacgactg gcgcagcatc cgccagccgt gcgacgcgtt cgtttcggtc gggatgctgg
     3901 agcacgtcgg cctctccaat tatcagcagc tcggggcggc gatcggccgc tgcctgacgc
     3961 cgagcggccg cggcttgatt cactcgatcg gccagaacca gccgatgccg atgaacccct
     4021 ggatcgagcg gcggattttc cccggcgctt acccgccgac gctccgccaa tgtatggatg
     4081 tgctggagcc gcacagcttt acagtgctag atgtcgagaa tctgcgactg cactatgcgc
     4141 tcacgctgag gcattggctt gcgcggtttg agctggcatc ggaaactgtg aagcagcgtt
     4201 tcggtgaaaa atttgtgcgt acttggtgga tgtatctggc gggctcgatg gcagcctttg
     4261 aaagcggcac gttgcagctc tttcagatca cgtttgcccg gggtgattgc aaccgggttc
     4321 cctggactcg ggcggagcta tatgcccagc cgggggccag ccctccgggg caatgggcgg
     4381 gtaacaagtg acggggaaag aactgccgga ccgtgccgtt cgtctattgg aatggtctgg
     4441 tgctggaaat ctgccgcggg gctaacaaaa atagaactat tcccaacttc acgcctttgg
     4501 gtgactaaca tgcgaatcgc ggtactcgcg gctgccagtg ttgctggcct gattttgacg
     4561 acgtctgtgc tggctcaggt gaggattcgc gccgagcctg cggccatccc tgcggaagcc
     4621 aagccagccg atcccaacaa gccggccgaa cctgctgctg aagaacccgc catcgatgtc
     4681 ccgcgcaagc cgccagtgga gctgggaccc aagcacatcc gtctgcacct gttggacggc
     4741 agcgtcatta gcggcgacct ttccgtggct gagatcaccg ttgacacccc ctttggcagt
     4801 ctggtcgtgc cgatcgaccg cattcgcagc ctccggccag ggctggatag ctatccgaaa
     4861 gtgctggccg aatttgaagg actcattcag aatctgggct ccgacgatta tcagacccgc
     4921 gagcaggcgc acaaagatct ggccgccatg gggccgaagg tccgcaagga attggagcgg
     4981 ttcagcagtg acgagaatgc cgaaatcaag cggcacgtag gcgagatcct gaaggagctc
     5041 gaggagcaag gccaggaggc cgaggaattc gacg
//
LOCUS       DASUGB010000227         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_442968, whole genome shotgun sequence.
ACCESSION   DASUGB010000227 DASUGB010000000
VERSION     DASUGB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442968"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3035)
                     /locus_tag="VFV87_04425"
     CDS             complement(<1..3035)
                     /locus_tag="VFV87_04425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103030.1"
                     /translation="MALDLTCPAGHAFFVPQPTTTGSTSCPRCGQAVALPAWSTETCA
                     WMRSPVTDSTPAGGQRIGRFVLLNKVGEGAAGVVFRAYDPSLDRHVALKVPRAGLLDQ
                     QDDIAAFLREGLAAGKLRHPNIVPVHEAGQADGICYIASAFIEGRTLKSAIEESGRMP
                     PPQAAELVARLAAALHYAHERGVVHRDIKPGNIMLGADGEPQIMDFGLAHRAAGETLQ
                     TVDGVLVGTPAYMSPEQAAGRSSQVDARSDLWSLGVVLYELLTGARPFDGELGPLLSA
                     IQHREPIPPRKLKRSIPRDLETICLKCLAKSPAQRYASCDVLAGDLQRFGRGEPIVAR
                     PVSRLERTIRWCRRQPALAAALGLAGAFLIAAASLGGYHLAFRSAASQETERLAKDLG
                     SKEKLVLTAQSKASQEQQRADAESRAKQRQICENKLTRALELCAAGDIDHGLLLMADA
                     LNNAKSAGAADLDRVLRLNIAAWASRLITLEQVVRQPDSSVIYCQFSANGQRLVTGGS
                     VQYVWDLPRGAVVAKTRQREDFPLAIGWGPGDSTFLFGSTQRIAKCNVATGAPLFTLG
                     NPERGRWDGIAFSRDGRRVLLWKTFAKAAELWDVESGKRVAELAHDDIVSGAISPDRK
                     TVVTGGFLGQDIKMWNADTGEPLPVNFIAGTQPNHVQFTGDGRKLLACGGRMQLYDVV
                     SGKAAGKIMKPPPAARAFTDYGVDLTTDGRLVAVRGDDNRVHIFESLTGEEFPSPLEH
                     SGSVHRMAFSHDGRRLLTSSADGTARLWDAERSAGIGQPLRHGSPTFAVGFSPTSARF
                     VSGDRDGSARVWREPAPARRLETKLTNAFTSFGFRGTAFADPKVRIGSTGLVAVLDWN
                     QGLYSIRTWDGMTGKEGPRFAPNIQPHSIGMSLLAASADQTLIAIRWHDHSSRQSVQI
                     RQLAVDTGTEVRQPIVLPSNRYSSAHVEYSPDGEVLLASTTNGAALFNSRTGEQIGDW
                     LPPQSVPRRFSPDGKVLLCRASDGSMELRPWRRHDPRELA"
     gene            complement(3108..>3333)
                     /locus_tag="VFV87_04430"
     CDS             complement(3108..>3333)
                     /locus_tag="VFV87_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX5103031.1"
                     /translation="LRAPAAFQGKTPEQLALRSKYNVTLVAVKRANRESGESASPTFA
                     THVPHAGFVIEPGDLLMLVGPDSALKLLPN"
BASE COUNT          583 a         1110 c         1060 g          580 t
ORIGIN      
        1 gccaattctc tcgggtcatg ccttcgccac ggtcgcaatt ccatgcttcc atccgaggcg
       61 cgacacaata gaaccttacc gtcaggcgag aaacggcggg gtacgctctg cggaggcagc
      121 cagtcgccta tctgctcgcc ggtccgcgag ttgaatagcg cggctccatt cgtcgtggac
      181 gccagtaaaa cctctccatc cggtgaatac tcaacatgcg cgctgctata ccggttgctg
      241 ggcagaacga tgggctgacg aacttcggtt cccgtatcga cggcgagctg ccggatctgc
      301 acggactggc gcgacgagtg atcgtgccag cggatggcga tgagcgtttg gtctgcgctc
      361 gcggccaaga gggacattcc gatggaatgc ggctgaatgt tcggcgcaaa gcggggtcct
      421 tccttgcctg tcatcccatc ccatgtgcgg atcgagtaga gtccttgatt ccaatcgagt
      481 accgcaacca gtcctgtcga tccaattctc accttcggat cggcgaaagc cgtccctcga
      541 aaaccgaacg aggtaaacgc gttggtgagc ttggtttcca agcggcgcgc cggcgccggt
      601 tcgcgccaaa cgcgcgccga gccgtcgcgg tcaccgctaa caaatctggc cgacgttgga
      661 ctaaacccaa ccgcaaacgt aggcgaacca tggcggagag gctgtccgat tccagcactc
      721 cgctcggcat cccacagcct ggccgtgccg tccgcgctgg acgtgagcaa gcgccgccca
      781 tcgtgagaaa aggccatgcg gtgcacagaa ccgctgtgtt ccagtggcga agggaactcc
      841 tcgccggtca gcgattcgaa gatatgcacg cgattgtcgt cgccacgcac cgcgaccaga
      901 cgcccgtcgg tcgtgagatc gaccccgtaa tccgtaaagg cgcgcgcggc tggaggcggt
      961 ttcatgatct taccggccgc cttacctgat acgacgtcgt acaactgcat gcgtccgccg
     1021 caggcaagca gtttccgacc gtcgcccgta aactgcacgt gattgggctg agtcccagca
     1081 ataaaattca caggcaatgg ctcgcccgta tcggcgttcc acatcttgat gtcctgtccc
     1141 aaaaatccgc ccgtgacgac cgtcttgcga tcgggactga tcgcgccgct gacgatgtcg
     1201 tcatgcgcca gttctgccac tcgcttgcca gattccacat cccatagttc ggcagccttg
     1261 gcgaaagtct tccacaggag cacgcgccgg ccgtctcgac tgaacgcaat gccatcccag
     1321 cggccgcgct cggggttgcc aagcgtgaaa agaggtgcgc cggtcgccac gttgcatttg
     1381 gcgatccgtt gagtcgaacc gaacagaaaa gtggaatccc ctgggcccca gccgatcgcc
     1441 aagggaaaat cttcgcgctg ccgggtcttt gcgaccacgg cgccccgcgg cagatcccac
     1501 acatattgca cagatccgcc cgtcaccaag cgctggccgt tggcgctgaa ctggcagtag
     1561 atcacactgg aatccggttg ccgtacgacc tgctccagtg tgatcaggcg cgacgcccag
     1621 gcagcgatat tgagacgcag cactcgatcc agatccgccg cgccggcgct cttggcgttg
     1681 ttcagagcgt cggccatcag gagcaggccg tgatcgatgt cgcccgccgc gcatagctcc
     1741 agggcacgcg tcagcttgtt ctcgcagatt tgccgctgtt tggctcgcga ttcggcatcc
     1801 gcccgctgct gctcctgcga ggctttggac tgcgcggtca gaacgagttt ttccttgctg
     1861 ccaaggtctt tggccaggcg ctcggtctct tggctcgccg cggagcgaaa cgccaggtga
     1921 tagccgccca gagaggcggc cgcgatcaga aacgcgccgg ccagacccag cgctgcggcc
     1981 agcgccggct ggcggcggca ccagcggatg gtcctttcca gccggctgac ggggcgggcc
     2041 acgatcggct cgccgcggcc gaagcgctgc aggtcgcccg ccaggacgtc gcagctcgcg
     2101 tagcgctggg cgggcgactt ggccaggcat ttgaggcaga tcgtttccag gtcgcgcgga
     2161 atcgaacgct ttagcttgcg cggcgggatg ggctcgcggt gctggatggc ggagagcagc
     2221 ggacccaact cgccatcgaa cggccgagcg cccgtcagca gctcatacag cacgacgccc
     2281 aggctccaca ggtcgctgcg ggcatccacc tggctgctgc ggccggccgc ttgttcgggg
     2341 ctcatatagg cgggcgtgcc gaccagcact ccatccaccg tttgcagcgt ttcgcccgcc
     2401 gcgcggtgcg ccaggccaaa gtccatgatc tgcggctcgc cgtcggcgcc cagcatgatg
     2461 ttgccgggct tgatgtcgcg atgcacaacg ccgcgctcgt gggcatagtg cagggcggcg
     2521 gccaggcggg cgaccagctc ggcggcctgc gggggcggca tgcgaccgct ctcctcgatg
     2581 gccgacttca gcgtgcggcc ctcgatgaac gcgctggcga tgtagcaaat gccgtcggcc
     2641 tgccccgcct cgtgcaccgg cacgatgttg ggatgccgca gctttccggc cgcgagccct
     2701 tcgcggagga aagctgcgat gtcgtcctgc tggtcgagca ggccggcccg cggcaccttg
     2761 agggccacgt gccggtcgag cgagggatcg tacgcccgga agacgactcc tgccgcccct
     2821 tcgccgacct tattcagcag cacgaaccgc ccgattcgct ggccgccggc cggcgtgctg
     2881 tccgtgacgg gcgaccgcat ccaggcacac gtctcggtgc tccaggccgg caacgccacc
     2941 gcctggccgc agcgcgggca cgaggttgag cccgtcgtcg tcggctgcgg gacgaagaat
     3001 gcatgccctg ccgggcaagt cagatcgagc gccatgggtc cgcctcgctg ccaatggcga
     3061 ttgtagtcga tcgcggaacc cggctaaacg aaaaacaaga agacgcccta attcggcagc
     3121 agcttgagcg cggaatcggg tcccaccagc atcagcaggt cgccgggctc gatcacaaag
     3181 cccgcgtgcg gcacgtgcgt ggcgaacgtt ggggaggctg attcgcccga ctcgcgattg
     3241 gcccgcttca ccgctaccag cgtcacgttg tacttgctcc gcagcgccag ctgttcgggc
     3301 gtcttccctt gaaaggcggc cggcgcccgg agc
//
LOCUS       DASUGB010000228         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_303667, whole genome shotgun sequence.
ACCESSION   DASUGB010000228 DASUGB010000000
VERSION     DASUGB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303667"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFV87_04435"
     CDS             complement(<1..419)
                     /locus_tag="VFV87_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5103032.1"
                     /translation="MTSNEPLVDTLGRVHTNLRLSVTDRCNIRCFYCMPDENVRFKPR
                     SELLTFEEIERFARVAAGLGVNKLRLTGGEPLVRADLPKLVERLARIEGIHDIALTTN
                     GILLEEQAQALKDAGLTRVNISLDALSAETFRRIARR"
     gene            complement(416..835)
                     /locus_tag="VFV87_04440"
     CDS             complement(416..835)
                     /locus_tag="VFV87_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5103033.1"
                     /translation="MISLTSEPIDSAAVLAQVSSTDAGAVILFLGTTREFTHGRRTAS
                     LDYECYPEMAEKKLADLEAEARSRWPLTGCLIVHRLGHLELGEASIATAVSSPHRQAA
                     FEAGKWLIDTIKEVVPIWKRENWADGRSEWVHPGMSQ"
     gene            complement(838..1083)
                     /gene="moaD"
                     /locus_tag="VFV87_04445"
     CDS             complement(838..1083)
                     /gene="moaD"
                     /locus_tag="VFV87_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5103034.1"
                     /translation="MKLCVKLFAVAKERLGQNSIEIELPEQATVGMLRSSLVEQHPQL
                     AEVMRHTRLAVNSEYATDEAAIPARAEVALIPPVSGG"
     gene            complement(1080..>2423)
                     /locus_tag="VFV87_04450"
     CDS             complement(1080..>2423)
                     /locus_tag="VFV87_04450"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HEX5103035.1"
                     /translation="TDIKPLELHLTWTRELPQVAASCNGCGRCRTQSPDARMCPIFRL
                     APAEEASPRAKANLMRAITGGQLDPAALTTDELKSIADLCVNCHQCRLECPAAVDIPK
                     LMVECKAQYVTSNGLDTTDWVLTRLDSVAKWSSRLGPLSNWALHTRWTRWLLEKMVGI
                     AQGRKLPRVAGSSFIRRAERRRLTRPTRRTGGKVLYFVDLYANWYDVQLAEALVAVME
                     HNNIAVFVHPDQRPAGMARIALGDIERARRGAARNVRLLADAVRQGYEIVCTEPSAAL
                     CLTHEYVHLLDDEDARLVAEHTHEACAYLWKLHEAGKLELNFQPLNLALGYHMPCHLK
                     ALEVGSPGENLLRLIKGVNVRRVERGCSGMAGTFGLKRQNYRASLRAGWGLISAMRDP
                     AIQLGTTECSTCKMQMEQGTSKPTIHPLKVLALAYGLMPEIEPLLSARSEDLTVT"
BASE COUNT          404 a          757 c          801 g          461 t
ORIGIN      
        1 tcgcggcggg cgattctgcg gaacgtctcg gccgatagcg cgtccaggct gatattgact
       61 cgcgtcaagc cggcgtcttt caatgcctgg gcctgctctt ccaacaggat gccgttggtc
      121 gtgagggcga tatcgtggat gccctcgatt ctcgccagcc gctccaccag cttcggcaga
      181 tcggcccgta ccagcggctc tcctcccgtt agccgaagtt tgtttacgcc caagccggcg
      241 gcgactcgcg cgaaccgctc gatctcctcg aacgtcaaca gctcgctccg cggcttgaaa
      301 cgcacattct cgtccggcat gcagtagaag cagcggatat tgcagcggtc ggtgacgctg
      361 agccgcagat tcgtatgaac gcggcccaga gtatcaacta atggctcgtt tgatgtcatt
      421 ggctcatgcc agggtggacc cactcgctcc tcccgtccgc ccagttctcc cgcttccaga
      481 ttggcacgac ctctttgatg gtgtcaatga gccacttgcc ggcttcgaag gcggcctggc
      541 gatggggcga gctgacggcg gtggcgatgc tggcctcgcc cagctccaga tgccccagcc
      601 gatggacgat caggcagccg gtgagcggcc agcggctgcg ggcctcggct tcgaggtcgg
      661 cgagcttctt ctcggccatt tcggggtagc attcgtagtc gagcgaggcg gtgcggcgac
      721 cgtgcgtaaa ttcgcgtgtc gtgccgagga acaggatgac ggcgccagcg tcggtcgagg
      781 agacctgagc cagtacggcg gcggaatcga tgggctcgct ggtgagggag atcatgccta
      841 acctccgctc acgggaggaa tcaaggcgac ttcggcccgc gccggaatcg cggcctcgtc
      901 ggtggcatat tcgctgttca ccgccaggcg ggtatggcgc attacttcgg cgagctgtgg
      961 gtgctgctcg actagcgacg agcggagcat gccaaccgta gcttgctccg gcagctcgat
     1021 ctcgatgctg ttctgcccca gccgctcctt ggcgacggcg aagagcttga cgcaaagctt
     1081 catgtgacgg tcaggtcctc gctacgggcg ctgagcagcg gctcgatttc gggcatcagg
     1141 ccataagcca gcgccagcac tttcagggga tggatggtcg gcttgctcgt gccttgttcc
     1201 atctgcattt tacacgtcga gcactccgtt gtgccgagct gaattgccgg atcgcgcatg
     1261 gcgctaatca gcccccagcc ggcacgcagg ctggctcggt aattctgccg tttgaggccg
     1321 aaggtgccgg ccatgccgct gcagccgcgt tcgacccggc ggacattgac ccctttgatg
     1381 agccgcagca ggttctcgcc cggcgacccg acctccagag ccttgaggtg gcatggcatg
     1441 tgatagccca gagccagatt cagcggctgg aaattgagct ccagcttccc ggcctcgtgc
     1501 agcttccaga gataggcgca ggcttcgtgc gtatgttcgg ccaccagccg ggcatcctca
     1561 tcgtcgagca gatggacata ttcgtgcgtc aggcacaaag ccgcagaggg ctcggtgcag
     1621 acgatctcat agccctgccg cacggcatcg gccagcaggc ggacgttgcg ggcggcgccg
     1681 cggcgggccc gctcgatatc gcccaaggca atgcgggcca ttccggccgg ccgctggtcg
     1741 gggtggacga agacggcgat gttgttgtgc tccatcaccg caacgagggc ttcggcaagc
     1801 tgcacgtcgt accagttggc atacagatcg acgaaataca gcacctttcc gccagtgcgg
     1861 cgcgtggggc gcgtcaggcg gcggcgctca gcccggcgga tgaagctcga tccagcgacc
     1921 cgcggcaact tgcggccttg ggcgatgccg accattttct ccaggagcca gcgagtccat
     1981 cttgtatgta gagcccaatt ggagagcggc cccagccgcg acgaccactt ggccaccgaa
     2041 tcgagccgcg tgagaaccca atcggtcgta tccaggccgt tgctcgtaac gtactgggcc
     2101 ttgcattcga ccatgagctt cgggatgtca acggctgctg gacattcgag ccggcactgg
     2161 tggcagttga cgcacaggtc ggcgatcgat ttcagctcgt cggtagtgag cgcggctgga
     2221 tcgagctggc cgcccgtgat ggcccgcatc agattggcct tcgcccgcgg cgaggcctct
     2281 tcagccggtg cgaggcgaaa gatcgggcac atccgggcgt cgggcgattg cgtgcggcag
     2341 cggccgcagc cgttgcagct cgcagcgacc tgcggcagct cgcgggtcca ggtgaggtgc
     2401 agttcgaggg gcttgatgtc cgt
//
LOCUS       DASUGB010000229         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_860437, whole genome shotgun sequence.
ACCESSION   DASUGB010000229 DASUGB010000000
VERSION     DASUGB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860437"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..2504
                     /locus_tag="VFV87_04455"
     CDS             456..2504
                     /locus_tag="VFV87_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX5103036.1"
                     /translation="MSSGRHIISLVAQRQDLEQFRKKNWVGTFEDYLDLVRQKPEVAR
                     NAFERVYDMIIAGGTSVYEEMRGEKRMHFRFFDDPENEGRDAVYGLDESLEAFVNALK
                     SAAKGYGIEKRVLLMHGPVGSSKSTIARLLKRGLERYSATDEGALYTLGWTGDEPEVV
                     HWCPMHEEPLHLIPERFRDDVTANLNASRNEGEYRARIFGDLCPFCRFMYTERLKKYS
                     GDWTKVIQDVRVRRIVLSEQDRIGVGTFQPKDEKNQDSTELTGDINYRKIAEFGSDSD
                     PRAFNFDGEFNVANRGIIEFIEVLKLDVAFLYDLLGASQEHKVKPKKFAQTDIDEVIV
                     GHTNEPEYRRLQNNEFMEALRDRTVKVDIPYVTRLSDEIKIYEKDYNPKKVKGKHIAP
                     HTIEMAAMWAVLTRLEPPNNASLTLLQKLKLYNGKTLPGFTEENIKEMRAEATTEGMH
                     GISPRYVQDKISNALVAHLDSRSINPFMVMNELEAGLKHHSLITSDELRGKYRELLGV
                     VKEEYENIVKNEVQRAIAADEDALKRLCSNYIDNIKAYTQREKVRNKFTGQYEEPDER
                     LMRSIEEKIDIPDSRKDDFRREVMNYIGALMIDGRTFDYKSNERLHRALELKLFEDQK
                     DTIKLTSLVSNVVDAETQAKIDVVKSRLIRDFGYDDESATDVLNYVASIFARGDVKKK
                     "
     gene            2553..>3137
                     /locus_tag="VFV87_04460"
     CDS             2553..>3137
                     /locus_tag="VFV87_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX5103037.1"
                     /translation="MVMKIDRDASRFKQIVRGKIRENLRKYVTHGEMIGRKGNDLVSI
                     PLPQLDVPHFRYGQNGRGGVGQGEGEVGQPIASGDPDGEGGQGQAGSESAPHVMEVDI
                     TLEELAEILGDELQLPRIEPKGKANIQQDKVRYTSVRRIGPESLRHKRRTYVEALKRQ
                     LSEGSYRPMDPRVVPIKDDRRYRSWQHVPEPVANA"
BASE COUNT          737 a          970 c          951 g          479 t
ORIGIN      
        1 gaggcagatg aactgatcgg ttagggactg gaccggctca agcgacagga tgtcgtcctt
       61 ccctgcctat cctctcgcct ccaccccgag cgctctttcc caagttcgcc cgcgccactg
      121 cgagtcctcc gcagcgtcgg cattctttcc cggggtgtga gagtcctccc tcgcgaatcg
      181 gaatgcatcg tatttcgcaa gccactgggt tggctgcgca gtggccgccg tcattccaga
      241 gcaggctgcg ctggccgata ccgacggggg cgacgcttga tgatggaacc ttggggccag
      301 gcgagccgtc cgcccgagta ccgtggtccg aaaatccatc cgccgcagtt cttaacagaa
      361 cgcaggcggc tgaatcacaa gggggtgttg ccagaccagc ccgcgaagcc caataagctc
      421 gtcggctcgc cttccaaccc cgggaggaat ggagaatgag cagcggccgc cacattattt
      481 cgcttgtcgc ccagcggcag gacctcgaac agttccgcaa aaagaactgg gtcggcacgt
      541 tcgaggacta tctcgacctg gtccgccaaa agccggaagt cgcccgcaat gccttcgagc
      601 gggtgtacga catgatcatt gcggggggca cttcggttta tgaggagatg cggggcgaaa
      661 agcggatgca cttccgcttc ttcgacgacc cggagaacga aggccgggac gccgtctatg
      721 gcctcgacga atcgctcgaa gcgtttgtca acgcgctcaa gagcgcagcc aaggggtacg
      781 gcatcgaaaa gcgcgtgctc ctcatgcacg gtccggtcgg aagcagcaag agcacgatcg
      841 cccgcttgct caagcgtggc ttggagcgct attccgccac cgacgagggg gctctctaca
      901 cacttggttg gaccggcgac gaacccgagg tggtgcattg gtgcccgatg cacgaagagc
      961 ccctgcacct cattcccgag cgattccggg acgacgtgac ggccaatctc aatgccagcc
     1021 gcaatgaggg ggaataccgc gcccgcatct tcggcgatct gtgccccttc tgccgcttca
     1081 tgtacaccga gcggctgaag aaatacagcg gcgactggac gaaggtcatc caggacgtgc
     1141 gggtgcggcg aatcgtgctt tccgagcagg accggatcgg cgtcggcacc ttccagccca
     1201 aggatgagaa gaaccaggac agcaccgagc tgaccgggga catcaactac cgcaaaatcg
     1261 ccgagttcgg cagcgacagc gacccgcggg ccttcaattt cgacggcgaa ttcaacgtcg
     1321 ccaatcgggg catcatcgag ttcatcgaag tgctcaagct ggacgtggcg tttctctacg
     1381 acctgctcgg ggccagccag gagcacaagg tcaagccgaa aaagttcgcc cagaccgata
     1441 tcgacgaggt gatcgtcggc cacaccaacg agccggagta ccgccggctc cagaacaacg
     1501 agttcatgga ggccctgcgg gaccgcacgg tgaaggtgga tatcccgtac gtcacccgcc
     1561 tctcggacga gatcaagatt tacgaaaagg actacaaccc caagaaggtg aagggcaagc
     1621 acattgcgcc gcacacgatc gaaatggcgg ccatgtgggc cgtgctcacc cgcctggagc
     1681 cgcccaacaa cgccagcctg acgctgctgc aaaagctcaa gctctacaac ggcaagacgc
     1741 tgcccggatt cacggaagaa aacatcaagg aaatgcgggc cgaagcaacg accgaaggga
     1801 tgcacggcat ctcgccccgc tacgtccagg acaagatcag caacgccctg gtcgcccacc
     1861 tcgattcccg gagcatcaac ccgttcatgg tgatgaacga gctggaggcg gggctcaagc
     1921 accactcgct gatcaccagc gacgagctgc ggggcaaata ccgcgagctt ctgggcgtgg
     1981 tgaaagagga gtacgagaac atcgtgaaaa atgaagtcca gcgggcgatc gccgccgacg
     2041 aggacgccct caagcggctc tgctcgaact acatcgacaa catcaaggcc tacacgcagc
     2101 gcgaaaaagt tcgcaataaa ttcaccggac agtacgaaga accggatgag cggctgatgc
     2161 ggtcgatcga ggaaaagatc gacatccccg acagccgcaa agacgacttc cgccgcgaag
     2221 tgatgaacta catcggcgcc ttgatgatcg acggccgcac gttcgactac aagagcaacg
     2281 aacggctcca ccgagccctc gaactcaagc tgttcgaaga tcaaaaggac acgattaagc
     2341 tgacgagcct cgtgtcgaat gtcgtagacg ccgaaaccca agcgaagatc gacgtcgtga
     2401 agagtcgcct catccgagac ttcggctacg acgacgaaag cgcgaccgac gtgctgaact
     2461 acgtcgcgag catttttgcc cgcggcgatg tgaagaagaa gtaggtcgct tgactcaacg
     2521 acaagtgaca aatgacgaat gacaagtgac caatggtcat gaaaatcgac cgcgacgcgt
     2581 cgcggttcaa acaaatcgtc cgcgggaaga tacgcgagaa cctgcggaaa tacgtcaccc
     2641 acggcgagat gatcggccgc aagggaaacg acctcgtcag cattccgctg ccgcagctcg
     2701 acgtgcccca tttccgctac ggccagaacg gccgcggcgg cgtcggccag ggcgaaggtg
     2761 aagtcggcca gccgattgcg tccggcgatc ccgacggcga aggcgggcag ggccaggcgg
     2821 ggagcgagtc ggccccgcac gtcatggaag tcgatatcac gctggaggag ctggccgaaa
     2881 tcctgggcga cgagctgcaa ctgccgcgga tcgagcccaa gggcaaggcc aacatccagc
     2941 aggacaaggt ccgctataca agcgtccgcc gcatcggtcc cgaatcgttg cggcacaagc
     3001 gccgcacgta cgtcgaagcc ctcaagcggc agcttagcga gggcagctac cggccgatgg
     3061 atccccgcgt cgtgccgatc aaggacgacc gccgctaccg gtcgtggcag cacgtgcctg
     3121 aaccggtcgc caacgcc
//
LOCUS       DASUGB010000230         5493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_164556, whole genome shotgun sequence.
ACCESSION   DASUGB010000230 DASUGB010000000
VERSION     DASUGB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164556"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            528..2270
                     /gene="kdpA"
                     /locus_tag="VFV87_04465"
     CDS             528..2270
                     /gene="kdpA"
                     /locus_tag="VFV87_04465"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX5103038.1"
                     /translation="MTADGIFQLAFYMVVLLVAARSLGSFMARVYEGKPHLLSWLGGL
                     ESRLYRLAGVDPQGEMTWRQYAVAMLAFNLLGLVALYGLQRVQHLLPLNPANMPAVVP
                     DSSFNTAISFASNTNWQGYGGESTMSYLTQMLGLGVQNFLSAATGMAVLVALIRGFTR
                     TQTAGIGNFWVDLTRSTLYILLPLSLVLAIALVSQGVVQTFNPYATATLLDPTVDGDG
                     KAVTEQTIAVGPAASQIAIKQLGTNGGGFFNVNSSHPLENPTPLSNLLEMLAILLIPA
                     ALCTTVGEMVGDRRQGIAILATMVLLFLPLLVLCYGAEQSGNPQLSKLGADQVASAMH
                     SGGNMEGKEVRFGIANSALWATATTAASNGSVNSMHDSYTPLGGLVPMLLMQLGEVIF
                     GGVGSGLYGMFAFAIVAVFVAGLMVGRTPEYLGKKIEAFEMKMASLIILIPPAVVLIG
                     TAVAVVAPQGLATIHNAKAHGFSEVLYAFSSAGNNNGSAFAGLGANTLFYNMALGLAM
                     FIARYWLAIPILAIAGSLASKKNVPPSSGTMPTHSPLFVVLLASVVVIVGALTFLPAL
                     ALGPIAEQLKMYSP"
     gene            2330..4480
                     /gene="kdpB"
                     /locus_tag="VFV87_04470"
     CDS             2330..4480
                     /gene="kdpB"
                     /locus_tag="VFV87_04470"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX5103039.1"
                     /translation="MSTTASLRRRPLFDPIIVKRALKDAVLKLDPRNQVRNPVMFVVL
                     VGAVATTLLLVERLLTGGEITQNAAGKVDPNSPWFIAGISVWLWFTVLFANFAEAMAE
                     GRGKAQADALKATRQNVAAKKILEKVPNLTLRPDFPDLVKNGEATFAPAPDLRMGDIV
                     LVEAGDQIPGDGEVIGGVASVDESAITGESAPVIRESGGDRSSVTGGTRVLSDWLIVR
                     ITANPGESFLDRMIHLVEGAKRQKTPNEIALSILLSAFAIMFLLACATLLPFSIYSVN
                     SAGQGTAITVTVLIALLVCLIPTTIGGLLSAIGIAGMDRMIQANVIATSGRAVEAAGD
                     VNTLLLDKTGTITLGNRQAVEFLPAEGVTTDRLADAAQLASLADETPEGRSIVVLAKE
                     KYGLRGRDIQQLRATFVPFTAQTRMSGVNLDGRRIRKGALEAVAQHLGGVVADLPDKV
                     ICDSNKIAQQGGTPLVVAEGEKALGVIYLKDIVKGGIKERFAELRQMGIKTVMITGDN
                     PLTAAAIAAEAGVDDFLAQATPEAKLSLIREQQAGGRLVAMTGDGTNDAPALAQADVA
                     VAMNSGTQAAKEAGNMVDLDSNPTKLIEVVQIGKQLLMTRGALTTFSIANDVAKYFAI
                     IPAAFASTYPVLGQLNIMHLASPNSAILSAVIFNALIIIALIPLALRGVKYRFATAAT
                     LLRDNLLIYGLGGLIVPFIGIKLIDLALYYAHLA"
     gene            complement(4559..5020)
                     /locus_tag="VFV87_04475"
     CDS             complement(4559..5020)
                     /locus_tag="VFV87_04475"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX5103040.1"
                     /translation="MGRSRGGYSTKIHLLCDGQGHPLHAEVTAGQAHEATGFTTVLEN
                     ARITDYDGELDVSPIRLAGDKAFDAEWIREWLDERDITPVIPHRGEIPEARHPDFDRE
                     MYRRRNVIERLVGWLKECRRLFARYEKTALNFLGFIKMGFIHFYLRLFCPE"
     gene            complement(4939..5397)
                     /locus_tag="VFV87_04480"
     CDS             complement(4939..5397)
                     /locus_tag="VFV87_04480"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX5103041.1"
                     /translation="MARHRLTDEQWECIAPVFPPPANTGRPRSDLRQVVDGILWILRT
                     GAPWRDLPEEEYGPWETVYTWFNQWNSDGTLDEIVDRLKAAMTDSETFDHELWCIDGT
                     IVRAHRCAGGGGKKTTRASRKTTRWGVPAAAIRQRSTSSATAKATRCMPK"
BASE COUNT         1038 a         1714 c         1611 g         1130 t
ORIGIN      
        1 gcgccccctt ggcgttcaag gcgatcatga cgctgcccca gccgtcggcc ttcacgggct
       61 tggtccgtgg cttcgacggt caatgcccgt gctgccggcg gcctttcgca gatcgatcgt
      121 agacgcgggc gatgggaccg acgcgcgtaa tttgtagctg tgcctgtgtc ggccgaacct
      181 gtgtggatgc tctttgcgag ttgcctagga attgagcaag gtctccgttg cgaattgccc
      241 tcttagatgc gttgatgaat tcttgatgcg caggctcgca gtcttgacac tcttttgaca
      301 cggttttcgg tagcattcca tcggtgcctt tccgtggagg tgtgcgatga cggatgtcca
      361 atttctggtg ctcgtcgccg tgctaggggg cctttccctg ggactgatca gagtctgcag
      421 cgtgctgtca ggagaacagc catgatctac gcaattgccg gagttttctc agtgcttctg
      481 ctggtttact tgatcatttc gctgctgaag ccggagtggt tctgaatatg acagccgatg
      541 ggatatttca actggcgttc tatatggtcg tgctcctggt ggccgctcgg tcgctcggaa
      601 gcttcatggc ccgcgtctac gaggggaagc cgcacctact ttcctggcta ggcggcctgg
      661 agagccggct ctatcgcctg gccggcgttg atcctcaggg agagatgacc tggcggcagt
      721 acgccgtcgc catgctggcg ttcaacctgc ttggtctggt cgccctatac ggcctgcagc
      781 gcgtgcagca tctgttgccg ctcaatccgg ctaacatgcc ggccgtggtg cccgactcgt
      841 cgttcaacac agccatcagc ttcgccagca ataccaactg gcagggctac ggcggcgaat
      901 cgacgatgag ctacctgacg cagatgctcg gcctgggcgt gcagaatttt ctttcagctg
      961 cgacgggcat ggcggtgttg gtggcattga tccgaggctt cacgcggaca caaaccgccg
     1021 gaatcggcaa cttctgggtc gatttgacgc gcagcacgct ctatatcctg ctgccgttat
     1081 cactcgtcct ggcgatcgct ctggtcagtc agggcgtcgt gcagacattc aatccatacg
     1141 caactgccac gctgctcgat cccaccgtcg atggcgatgg caaagcggtc acggagcaaa
     1201 ccatcgcggt cggtccggcg gcgtcgcaga tcgcgatcaa acagctcggc accaacggcg
     1261 gtggtttctt caacgtgaac tcttcgcatc cgttggagaa tcctacgccc ctctccaacc
     1321 tactggagat gctggctatc ctgctcatcc ccgccgcact ctgcaccacc gttggtgaga
     1381 tggtcggcga ccgccgccag gggatagcga ttctggccac gatggtgctg ctcttcttgc
     1441 cgctgctggt cctgtgctat ggggccgagc aaagcggcaa cccgcaactc tctaagcttg
     1501 gggccgacca ggtcgccagc gccatgcaca gcggcggcaa catggaaggc aaggaagtcc
     1561 gcttcggaat cgctaattcg gcattgtggg ccacggcgac caccgccgcc agcaacggta
     1621 gcgtgaactc gatgcacgac tcctacacgc cgctaggagg cttggttccc atgttgctga
     1681 tgcaactcgg cgaggtcatc tttggcggcg tcggcagcgg cctgtacggg atgttcgcat
     1741 ttgccatcgt tgccgtcttt gtcgccggcc tgatggttgg ccgcacgcca gaatatctcg
     1801 gtaaaaagat cgaggccttc gaaatgaaaa tggcctcgct catcatcctc atcccgccgg
     1861 ccgtagtgct gatcggcacc gccgtcgccg tcgttgctcc gcagggactg gcgacgatcc
     1921 ataacgccaa ggcacacgga tttagcgaag tcctctacgc cttctcctca gccggcaaca
     1981 acaacggtag cgcatttgct ggcctcggcg cgaacacgct cttttacaac atggccctag
     2041 gcctggcgat gtttattgcc cgctactggt tggcaatccc gatcctggcc atcgccggct
     2101 cgctggccag caagaagaac gtgcctccca gtagtggcac aatgccgacg cattctccac
     2161 tgtttgtcgt attgttggcc agcgtcgtcg taattgtcgg cgcactaacc tttctgcctg
     2221 cactggcgct gggaccaatt gccgaacagt tgaagatgta ttccccgtga cccgcgcgta
     2281 aacacagtgc gcatgctgcg atcaaccaac aagttcttca gtcacttcca tgtccacgac
     2341 cgcttcgctt cgtcgccggc ccttgttcga tccaatcatc gtcaagcgag ccctcaaaga
     2401 cgcagtcctt aagctcgatc cacgaaatca ggtccgcaac ccggtgatgt tcgtcgtcct
     2461 tgttggagcg gtcgccacga ctttgttgct cgtcgagcga ctcctcaccg gcggcgaaat
     2521 cacccagaat gcggccggca aagtcgatcc gaactcacct tggtttattg ccggcatcag
     2581 cgtctggctt tggttcaccg tgctcttcgc caactttgcc gaagcgatgg ccgagggccg
     2641 cggcaaggcg caggcggacg cgctgaaggc cactcggcag aacgtggccg cgaagaagat
     2701 tctagagaag gtccccaatc tcacactgcg ccctgatttc ccggacctgg tgaaaaatgg
     2761 cgaagcgacg ttcgcccctg cccccgacct gcgcatgggc gacatcgtcc tcgtcgaagc
     2821 gggcgatcag attcccggcg acggcgaagt gatcggcggc gttgccagcg tcgatgaaag
     2881 cgccatcacc ggcgaaagcg cccccgtcat tcgcgaaagc ggcggcgacc gcagcagcgt
     2941 cactgggggc acgcgggttc tctccgactg gctgatcgtg cgaatcactg ccaaccccgg
     3001 cgagagcttt ctcgaccgga tgattcacct cgtcgaaggc gccaagcggc agaagacgcc
     3061 caacgaaatt gccttgagca tcttgctttc ggcctttgcg atcatgtttc tcttggcctg
     3121 tgcgacgctc ctgccgttct cgatttacag cgtgaattcg gccggccagg gaactgccat
     3181 taccgtgacc gtattgattg ccctgctcgt ctgcctcatc ccgacgacga tcgggggtct
     3241 gctttcggcg atcggaatcg cagggatgga ccgcatgatc caggccaatg tgatcgccac
     3301 cagcggccgc gctgtggaag cggctggcga cgtaaacacg ttgctcctcg acaagactgg
     3361 cacaatcacg ctgggcaacc gccaggcggt cgaattcctg ccggccgagg gggtcacgac
     3421 cgaccgcctg gcagatgcag ctcagctcgc ctcgctggcc gacgaaacac cggaaggccg
     3481 cagcatcgtt gtcctcgcca aggagaaata cggtctgcgc ggccgtgaca tccagcaact
     3541 ccgagcgaca ttcgtgccgt tcaccgcgca aacgcggatg agcggtgtga acctcgatgg
     3601 ccgccggata cgcaaaggcg ctctcgaagc cgttgcccag cacttgggtg gcgtggtcgc
     3661 cgacctgccc gacaaggtca tctgtgattc aaacaagatc gctcagcaag gcggcacgcc
     3721 cctggtggtg gctgagggag agaaagccct gggagtgatt tatctgaaag acatcgtcaa
     3781 agggggcatc aaggaacgct tcgccgagct ccgccagatg ggaatcaaga ccgtgatgat
     3841 cacgggcgac aacccgctca ctgctgccgc cattgcggcc gaggcgggcg tcgatgattt
     3901 cttggcccag gcgactcccg aagccaaatt gagcctcatc cgcgagcagc aagcaggcgg
     3961 acgcctggtt gcgatgaccg gcgatggcac caacgacgct cccgctcttg ctcaggctga
     4021 cgtggccgtg gcaatgaaca gcggcacgca agccgccaag gaagcgggca acatggtcga
     4081 tctcgacagc aacccgacga aattgatcga ggtggtgcaa atcggcaagc aactcctgat
     4141 gacccgcggg gcgctgacga cgttcagcat tgccaacgac gtggccaaat acttcgcgat
     4201 catcccggct gcgttcgcca gcacgtaccc agtactcgga cagctcaaca tcatgcacct
     4261 ggcgtcgccc aacagcgcaa tactatcagc cgtgatcttc aacgcgctga tcatcatcgc
     4321 cctgattccg ctggcgctcc gcggcgtgaa gtatcgtttc gcgaccgccg ccacgctgct
     4381 ccgcgacaac ctgctgatct acggcctcgg cggcctgatt gtgccattca tcggcatcaa
     4441 gctgattgac ctggcgctgt actacgctca cctggcgtga aagtggtcag gaaattcctt
     4501 cacattgatc cacctatagc catcatgtcc cgcgacattc taggctccgt ttgaaaactc
     4561 attctgggca gaacagtcgc aagtaaaaat gtatgaagcc cattttgatg aagccgagaa
     4621 agttgagcgc cgttttctca taacgggcga acagtcggcg gcattccttg agccagccaa
     4681 ccagccgctc gatgacgttg cggcgacggt acatttcgcg gtcaaagtca ggatgccgcg
     4741 cctcgggaat ctccccgcga tgcggaatga ccggcgtgat gtcgcgctcg tcgagccact
     4801 cgcgtatcca ctcggcatcg aacgccttgt cgccggccaa gcggatcgga gaaacgtcca
     4861 gttctccgtc gtagtcggtg atgcgggcgt tttccagaac cgtcgtgaag cccgtggcct
     4921 cgtgtgcctg gccggcggtc acttcggcat gcagcgggtg gccttggccg tcgcagagga
     4981 ggtggatctt tgtcgaatag ccgccgcggg aacgccccag cgcgtggtct ttcggctcgc
     5041 gcgggtcgtt ttttttccac caccgccggc acaccgatgg gcgcggacga tcgtgccatc
     5101 gatgcaccac agctcgtggt cgaacgtctc gctgtcggtc atcgccgctt tgagccgatc
     5161 cacgatctcg tccagcgtgc catcggagtt ccactggttg aaccaggtat acacggtctc
     5221 ccacggaccg tattcctcct cgggcaaatc ccgccacggc gctcccgtgc ggagaatcca
     5281 cagaatgccg tccacgactt gccgcaggtc ggaccgcggc cgccccgtat tggctggcgg
     5341 cgggaaaacc ggggcaatgc attcccattg ttcgtccgtg aggcgatgcc gggccatagc
     5401 gcactccctc cttggagcat cgaggatcga tgacgatggc ccttagcatt gcttaaacca
     5461 gcagtatcag caaagatcag ttttcaaacg gag
//
LOCUS       DASUGB010000231         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1555853, whole genome shotgun sequence.
ACCESSION   DASUGB010000231 DASUGB010000000
VERSION     DASUGB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555853"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2761
                     /locus_tag="VFV87_04485"
     CDS             <1..2761
                     /locus_tag="VFV87_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5103042.1"
                     /translation="RILMLDDFDPATGKARKITTFFEGTTHTMAIALYHDGSFYVATR
                     MEIFRLRDKDGDGKADERTPICHLDTPGNYPHNGLSDFAFDFAGNVYFGFGENLGAEY
                     KLIGSDGKTLSGGGEGGNIYCCDPEGKNLRRVATGFWNPFALGFDAFGRLFAVDNDPD
                     SRPPCRLLHIVEGGDYGFRFRNGRKGVHPFTAWNGELPGTLPMVAGTGEAPCAVMAYE
                     SDNLPEEYRGDLLVTSWGDHRLERYTLRPRGASFGAEMTQVIRGGENFRPVGLALAPD
                     GSLYMSDWVDKSYNVHGKGRVWRISARNPPQRPAFKDAWEALASKHGPTREAALRQIP
                     RVAENRLRAVQVMLQSPDNAVQAAAVRAVDAEDFFSQSLLLGLLGSHPSASVRTAILG
                     HLRASATNNADWKQLELRPEWVKGFSDNRALPPETVAQILAHTQLRSFPLALLYRNVQ
                     HYYDRESYGAGLRQDPKFWAVIAQDPFLLNAYTSAIASDIVDAKVTRLRMRQIGRIDS
                     KIAEERLVLALAAKRAGDVELPELVPDLLVDSDLRVQLVGLLWLADENPNRLEPFRWR
                     VESLLKRPNITRQVFEVAVAVLNRIDGTNPDPKNESAGDAFVLKMLLDEKAAPATRRF
                     ALRSLRPDHPSLTVEILTKLLADPEESIRLEAIRSLRQRPDSERWPQLREIAADSTAP
                     VQLRCEAILGLSPGNGEDRKLLFELTSCDAAEVADEALRALRGFDLEPDEKEQLTALA
                     AKLEGPRKELAERVLMRNPPKNLPKHEDAEAWLKMFEGPGNPLAGERIFYHLRVGGCF
                     RCHEFEGRGYTIGPDLSTIGRSLTRERLVQSLVDPSREIAPQFTSWAIQTTGGEVLTG
                     IHVGDEVDGRLKFADQNGRVFHVHPNDIDQRKPSSQSIMPEGLVDNLTPQELRDLIAF
                     LLRTK"
     gene            complement(2871..3605)
                     /locus_tag="VFV87_04490"
     CDS             complement(2871..3605)
                     /locus_tag="VFV87_04490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5103043.1"
                     /translation="MSRRSLITGSLLFVAGLLGYVAGLGQAPGQAQEKPPELKTLEEQ
                     AAYAIGLDIGKSILADGAELNPELVAKGLTDAMKKSKPLLTDAQIKEVMTAFTKAMQA
                     KADAKAKTAGEKNMKDGATFLAANKAKKGVKTTDSGLQYEVLKAGTGTVSPKKTDVVR
                     VHYHGTLIDGKVFDSSVERKEPAEFPVNRVIAGWTEALQLMKVGDKWKLTIPADLAYG
                     ERGTPGGPIPPNAVLVFEVELLEIVD"
BASE COUNT          705 a         1185 c         1203 g          714 t
ORIGIN      
        1 ccgcattctg atgctggacg acttcgatcc ggcgacgggc aaggcccgca agatcacaac
       61 cttcttcgag gggacgacgc atacgatggc gatcgccctc tatcacgacg gctcgttcta
      121 tgtcgcgacg cgaatggaaa tcttccgcct ccgcgacaag gacggcgacg gcaaagctga
      181 cgaacgaacg ccgatctgcc acctcgatac gcccggcaac tatccgcaca acggcctctc
      241 ggacttcgcg ttcgattttg ccggcaacgt ctatttcggc ttcggcgaga acctgggggc
      301 ggaatacaag ctgatcggca gcgacggcaa gacgctcagc ggcggcggcg aggggggcaa
      361 catctattgc tgcgatccgg aggggaaaaa cctccgccgc gtcgccaccg ggttctggaa
      421 cccgttcgcc ctcggcttcg acgctttcgg ccgcctcttc gccgtcgata acgaccccga
      481 cagccgcccg ccctgccgcc tgctgcacat cgtcgagggg ggcgattacg gcttccgctt
      541 ccgcaacggc cgcaagggag tacacccctt cacggcctgg aacggcgagc tgcccggcac
      601 gctgccgatg gttgccggca cgggcgaagc cccctgcgcg gtcatggcct acgaatcgga
      661 caacctgccg gaggaatacc gcggcgacct gctcgtgacg agttgggggg accatcgcct
      721 ggagcggtac acgctccgcc cccgcggcgc gtcgttcgga gccgaaatga cgcaggtgat
      781 ccgcggcggc gaaaacttcc gccccgtcgg cctcgccctc gcccccgacg gcagcctcta
      841 catgagcgac tgggtcgata agtcgtacaa cgtccacggc aaagggcgcg tgtggcggat
      901 tagtgccagg aatccgccgc agcggccggc gtttaaggac gcttgggagg cactcgcgtc
      961 gaagcacgga ccaactcgcg aggcagcact gcgccaaatc ccgcgagttg cggagaaccg
     1021 cctgcgtgcc gtgcaagtga tgcttcagtc gccggacaac gccgtacaag cagcagccgt
     1081 ccgcgctgtc gatgccgaag atttcttcag tcaatcactg ttgctcggac tcttgggaag
     1141 ccacccttcg gccagcgttc gcacggcgat ccttgggcac ctgcgagcca gtgcaacgaa
     1201 caacgcagac tggaaacagc tcgaattgcg tccggaatgg gtcaaagggt tttcagacaa
     1261 tcgcgcattg ccgcctgaga cggtcgcgca gattctggcc catacacaat tgcggtcatt
     1321 tccgctggcg ttgctctatc gcaatgtaca gcactattac gatcgcgagt cgtatggtgc
     1381 aggcctccgg caggatccga agttctgggc cgtcatcgct caggatcctt ttcttctcaa
     1441 cgcatacaca tctgccatcg cgtccgacat tgttgacgcc aaggtgactc gccttcgaat
     1501 gaggcaaatt ggtcgcatcg actcgaaaat cgccgaggag cgcttggtgc tggccctggc
     1561 agcgaaaagg gcaggcgatg ttgagctgcc tgagcttgtt cctgatttgc tcgtcgattc
     1621 ggacctgcga gtccaactcg tgggactctt gtggttggcg gatgagaatc caaaccgcct
     1681 tgagcccttc cgttggcgcg tggagagctt gttgaaacgg ccgaacatca cgcggcaggt
     1741 gtttgaggtg gccgtggcgg tcctgaatcg catcgatggc acgaatcctg atccgaagaa
     1801 cgaatcggct ggcgatgcat tcgtgctgaa gatgctgctc gatgagaaag cagcgccggc
     1861 aacgcggcgc ttcgctctgc ggtcgctcag gccggatcac ccatctctga cagtagagat
     1921 actgaccaag ttattggccg atccggagga gtcgattcgg ttggaggcga ttcgcagctt
     1981 gcggcagcgg ccggatagcg agcgctggcc gcagttgcgg gagattgcgg cggacagcac
     2041 agcgccggtg caattgcggt gcgaggcgat tctgggcctc tcgcccggca acggcgaaga
     2101 tcgcaagctt ttgtttgaac tgactagttg cgacgccgcc gaagtcgccg acgaggccct
     2161 gcgggcgctc cgcggctttg atttggagcc cgacgagaag gagcagctca cggctttggc
     2221 ggcgaagctg gaaggcccgc ggaaagagct ggcagaacgg gtcctgatgc gtaatccgcc
     2281 gaagaacctg cccaagcacg aggatgcgga ggcctggctg aaaatgttcg agggaccagg
     2341 caatccgctg gccggcgagc ggattttcta tcacctccgc gtcggcggct gcttccgctg
     2401 ccacgaattc gagggccgcg gctacacgat cggccccgac ctctccacta tcggccgcag
     2461 cctgacccgc gagcggctcg tccagtcgct ggtcgatccc agccgcgaaa tcgcaccgca
     2521 attcacgagc tgggccatcc agacgacggg cggcgaggtg ctcaccggca tccacgtcgg
     2581 cgacgaagtc gatggccggt tgaaattcgc cgatcaaaac ggccgcgtct tccacgtcca
     2641 ccccaacgac atcgaccagc gcaagccatc gagccaatcg atcatgcccg agggcctcgt
     2701 cgataacctc accccccaag agctccgcga cctgatcgcg ttcctcctcc gcacaaaata
     2761 gccgggacca aacctggtcc cggcgatcgg agcgacagag cttgaacctc gcgcgatcat
     2821 ccaattcgcg cgagtggcac gccgggacca ggtttggtcc cggctaaggg ctagtccaca
     2881 atctccagca gctcgacttc gaacaccagc acggcgttgg gcgggatcgg cccgccgggg
     2941 gtgccgcgct cgccgtaggc aaggtcggcg ggaatcgtca gcttccattt gtcgccgact
     3001 ttcatgagct gcaaggcctc ggtccagccg gcgatcacgc ggttaaccgg aaactcagcc
     3061 ggctctttgc gctcgacgga gctgtcgaag accttgccgt cgatcaatgt gccgtgatag
     3121 tgaacgcgga ccacgtcggt cttcttgggc gataccgtcc ccgtgccggc cttcagcact
     3181 tcgtactgca ggccggaatc ggtggtcttc accccttttt tcgctttatt ggcggccaga
     3241 aacgtggctc cgtctttcat attcttctcc cctgcggttt tggctttggc gtcggctttg
     3301 gcttgcatcg ccttggtgaa ggcggtcatg acttccttga tttgagcgtc ggtcaggagc
     3361 ggcttgctct tcttcatcgc gtcggtcagc cctttggcaa ccagttccgg gttcagctcg
     3421 gctccatcgg cgaggatgct cttgccgatg tccaggccga tggcataggc ggcttgctct
     3481 tccagggttt tgagttccgg gggcttttcc tgggcctggc cgggggcctg ccccaggccg
     3541 gcgacgtagc ccagcaggcc ggcgacaaag agcaacgagc cggtaatcag cgagcggcgc
     3601 gacatgagag cgatcctatt tggcaaggga aagccgcagc gggaagggaa gtcgctgcgg
     3661 cgtgggaagt tggttgatcc aggtaagtta tcctgccggc gcggcgttgg gcaaggtgtc
     3721 gcgcggaata ggcggttctg aagggaaggg cgcaactcga gcgaggaaga actgcctcgc
     3781 ttacgcttcg ggttactttg cgcggcg
//
LOCUS       DASUGB010000232         6885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_443434, whole genome shotgun sequence.
ACCESSION   DASUGB010000232 DASUGB010000000
VERSION     DASUGB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443434"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFV87_04495"
     CDS             complement(<1..323)
                     /locus_tag="VFV87_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103044.1"
                     /translation="MFPSLRKLAAILVATLLAGTANLALAQRLPCGDPCNPPIYGVDC
                     RMGDGCGEPHWKAWGPIPWQAFAQGEYVGPARLSHVPEYHLRVDDEIEFIYRLTREEL
                     AHPYLL"
     gene            complement(331..1794)
                     /locus_tag="VFV87_04500"
     CDS             complement(331..1794)
                     /locus_tag="VFV87_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103045.1"
                     /translation="MPALLPRCALFPAVLAAGIALAGCEQISPSAPDRAGAVPAEAPQ
                     SSTPQTLTLPDRVLPITAADYPAASGVALDSIAAGFEQSGAYAGGGARIRELSAAAES
                     ISPDVIREPTPGVIREPAPVAETVAEPIKLRPTEVPKPSESAPSLRNTPFAPINSSVE
                     SSSPTAEASPNFALNSPYAIDSRAQPDPAPALTPTPIPPAAFRPDRAAMQPVAQRAAE
                     ISNHAYSLAQRGMLYAARAELIRSLQLVAQALDVQYGSASHDAALAAGLAALKEARDF
                     AALSPRPGAVEEIVRGHQTPVLKSAAEVSPVVAQQQYLAYAQTQLSLAAGQEPVASLT
                     LFRLGKVQMAMAQQDGDSNMLHAPQAMVFYQAALATDRANYLAANELGVLLAGYGQLQ
                     QARELLVHSVSIQPHVEGWHNLAVVHRRLGETDLARRADHERQLLAQRSPPAIADTAN
                     NIAWVDAKTFAATSRSERPQTASQPNVRGSASIGTTR"
     gene            complement(1922..2896)
                     /locus_tag="VFV87_04505"
     CDS             complement(1922..2896)
                     /locus_tag="VFV87_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HEX5103046.1"
                     /translation="MFHFDGGLKITSIDLAVDFRRRQPRGFISHAHTDHMGRHELAYC
                     TPATAALYQRRYGPRPTRLMLFGEPLQWNGLTLTALPAGHVFGSAMLHVSGEQGTLLY
                     TGDFKLRPSATAEAATLPRADVLIMECTYGTPRYRLPPRDEAIAQLLMIVQRVLAAGR
                     TPVVRAYVLGKAQEVTRILTAAGLRVVQHPLVHEISLVYQQCGCDLGTFERLDGPPPE
                     DAVVVAPPRNQKAAHMIGLRRPVSIAVSGWAVDPGCRWLAADYAVPLSDHADFDELVE
                     CVERTEPAIVFCTHGPAGFVEELRRRGHNAHPLEDCRMVQTCGSFRGG"
     gene            complement(2988..4169)
                     /locus_tag="VFV87_04510"
     CDS             complement(2988..4169)
                     /locus_tag="VFV87_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5103047.1"
                     /translation="MAAGADRQELCHPLQHREDQDLPPRKRHASRTSRQIGVLSNRRR
                     WDFPQTIRSASRLPTHPEGHPVDPSGGAAIMGGILSDGLLLWAQKRPLTGTKEERMYK
                     CLSPSAIGVIGRQSELVEIALTHRFKGLEIDITELTKRAQTTGVPQACRYLSSAHMAI
                     GGFELPVRWAGEEQEFAADLAQLPLLLEVASTLHADRCFTSIRPTCDQRPFHENFKFH
                     TERLQKLADALAPGNVKLGLVLLAAPADRADGGFEFIHQVEPLLTLLNSVQRENVGLV
                     LDTWSWFVGGGDLEKVRTLKAEQILSVRLADLPADADLAAITSQQRLMPGEGGAIDCA
                     ALLGILEELGYDGPVTVAPDPESSKGQTREATITRASTALDGLLAGVSGETAVATAEK
                     S"
     gene            4086..4880
                     /locus_tag="VFV87_04515"
     CDS             4086..4880
                     /locus_tag="VFV87_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5103048.1"
                     /translation="MPFSWWQILILAVLQGVAEFLPISSSGHLVVLAPLLFGTSEAPT
                     DMTAVTVALHIGTLGSILVYYNRRIARLLGEDRGTLGLIVIGSIPAAVVGLLIELGNE
                     QIIEQPLLAGCMLPVTGGVLLWMSRRPAGTTEYRDMSWRSALVVGLSQAAAILPGLSR
                     SGLTIAAGVGTGLSRASAAAFSFLLAIPVIGGAGLLKLLLTDQQPATPVSLLFMGGGV
                     AFIVGIAALWCLDRMLQRGRLHWFAWYCIALGLAVIAWQLSAIFNP"
     gene            complement(4860..5885)
                     /locus_tag="VFV87_04520"
     CDS             complement(4860..5885)
                     /locus_tag="VFV87_04520"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX5103049.1"
                     /translation="MRPLVVTLATFAAAAILLGTPDVAPAADDESADAILARHELTRL
                     DRVWVLPLELELRQDLAELPKRRERIVGLERELDQRIEQNRLAWQDSRGRVAALKQSV
                     AKLSSNDPQRVVLQQQIDALEAAVTEPGRIGSRAEVRTSAVQWTAERNDLAAATVRVR
                     RALPKLLDQYMELGQRQEVQSALGRVGQGQRLGPQRSYRGDMRNLGEYERLVFTPWHP
                     LHLQAGQHRLTGLVDELSPLTFTWVDSGTQTTMVSATAAEAAGISVPEDAPRETVFLS
                     AGRSVVARRVTIPYLRFSGCVLRDVAALVLPPEAEDWGSRIGREALSGHSVRLEPERL
                     RLWIENG"
     gene            6097..6357
                     /locus_tag="VFV87_04525"
     CDS             6097..6357
                     /locus_tag="VFV87_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103050.1"
                     /translation="MWRAVFLAIGISMCIAGGECLVVEKFVIGAESPPPTPQTPATLF
                     GAPPSGTGPAARDIEPADWTPWSLLSAGAVVILYAITLNRQG"
     gene            6605..>6885
                     /locus_tag="VFV87_04530"
     CDS             6605..>6885
                     /locus_tag="VFV87_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5103051.1"
                     /translation="MKRISLLAPLAFFLLAGSAFGQGVLIIIDPPHPAPLPRPIIRPV
                     PTPPPMSYKIKELDYYARVVDQVAQVQVTQSFVNTGSQQMEVSFVFPLP"
BASE COUNT         1177 a         2185 c         2272 g         1251 t
ORIGIN      
        1 agcaggtacg ggtgcgcgag ctcctcgcgg gtcaggcggt agatgaactc gatctcgtca
       61 tcgacgcgga ggtgatactc cggcacgtgc gacaggcggg ccgggccgac gtattccccc
      121 tgggcaaatg cttgccaggg aattggcccc caggccttcc agtgcggctc gccgcagccg
      181 tcgcccatgc ggcaatcgac gccgtaaatg ggcggattgc aggggtcgcc gcagggaagc
      241 cgctgggcga gggcgagatt ggccgttccg gctagtaagg tggcgacaag gatcgcggcg
      301 agtttgcgga gcgatgggaa catggcgcgg ttaccttgtg gtgccgatgg aggcgctgcc
      361 gcggacgttt ggctggctgg cggtttgggg acgttcgctg cgactggttg ccgcgaaggt
      421 ttttgcatcg acccaggcga tgttgttggc cgtatcggca atcgcggggg gcgagcgctg
      481 ggcgagaagt tgccgctcgt ggtcggcgcg gcgggccaaa tctgtttcgc ctaggcgacg
      541 atggacgacg gccaggttgt gccaaccttc cacatgcggc tggatcgaga cgctgtgaac
      601 gagcagctca cgtgcctgct ggagctgccc gtacccggcc aagagcacgc ccagctcgtt
      661 tgccgccagg taattcgccc ggtcggtggc cagcgctgcc tggtaaaaga ccatcgcctg
      721 cggggcgtgc agcatgttgg aatcgccgtc ctgctgggcc attgccatct gcaccttgcc
      781 caagcggaag agcgtgagcg aggcaactgg ctcctggccc gcggccagcg aaagctgcgt
      841 ctgcgcgtaa gccagatact gctgctgggc gacgacgggg gaaacctcgg cagccgactt
      901 gaggaccggc gtctggtggc ccctgacgat ttcctcgacc gcacctggtc gcggcgagag
      961 cgcggcgaag tctcgcgcct ccttaagagc cgctaatcct gccgcgagcg ccgcatcgtg
     1021 cgacgcggat ccgtactgaa cgtccagcgc ctgcgccacg agttgcagcg agcggatcag
     1081 ctccgcccgc gcggcataga gcatgccgcg ctgggccagg ctgtaggcat gattggaaat
     1141 ctcggctgcg cgctgggcaa caggctgcat cgcggcgcga tccgggcgga acgcggcggg
     1201 cggaatgggc gtgggagtaa gagcaggagc aggatcgggc tgtgcgcgcg aatcgattgc
     1261 gtagggtgaa ttcagtgcga aattgggact cgcttcggcg gttggcgacg atgactccac
     1321 cgatgagttg atcggggcga agggcgtatt gcggagggag ggcgcagact cggatggctt
     1381 agggacctct gtcggcctga gtttgattgg ttccgcaacc gtttcggcga ctggcgccgg
     1441 ctcgcggatg acgcccggtg ttggctcgcg gatcacgtcg ggggagatcg actccgcagc
     1501 ggctgacagc tcgcggatcc gtgcccctcc gccggcataa gcgcccgatt gctcaaagcc
     1561 ggctgcgatg gaatcgaggg cgacaccgga agcggccgga tagtcggccg cggttatggg
     1621 caacacgcgg tcggggagcg tcagcgtctg cggtgtggag gactgaggcg cttctgcagg
     1681 cacggcgccg gcccgatccg gcgcggaggg cgagatttgc tcgcatcccg ccagggcgat
     1741 tccagcggca agaaccgctg gaaacagagc gcatcgtgga aggagcgcgg gcataggcca
     1801 tccgtgggcg ggcacgcggg agtagcgcga agcgacctcc gagtcgttgc actcgttacc
     1861 gtcatcgacc gctggcgaca aatggcagca ggcaaagggg cggctggcgc acggcggcta
     1921 atcatccgcc gcggaacgac ccgcacgttt ggaccatccg gcaatcttcc agtggatggg
     1981 cgttgtggcc ccgccggcgc agctcttcca caaatcccgc gggaccgtgc gtgcaaaaca
     2041 caatcgccgg ctcggtgcgc tcgacacact cgacaagctc gtcaaagtcg gcatggtcgg
     2101 acagcggcac ggcataatcc gcggccagcc agcggcaacc cggatcgacc gcccagccgc
     2161 tgaccgcgat cgacaccggc cgccgcaagc cgatcatgtg agccgccttc tgattccgcg
     2221 gcggagcgac gacgacagca tcttccggcg gcgggccgtc cagtcgttcg aacgtgccca
     2281 gatcgcaacc gcactgctga tacacgagac tgatttcgtg gaccagcgga tgctgaacga
     2341 cccgcagccc cgccgccgtg agaattcgcg tcacttcctg ggccttgccg agcacatagg
     2401 ctcgcaccac gggtgtacga ccggctgcga ggactcgctg cacgatcatc aggagctgag
     2461 cgatcgcctc gtcgcgcggc ggcaggcggt agcgcggggt tccatacgtg cattccatga
     2521 tgagcacatc cgctcgcggc aaggtcgcag cttcggcggt ggcggaaggg cggagcttga
     2581 aatcgcccgt gtagagcaac gttccttgct cgccggaaac gtgtagcatc gccgaaccaa
     2641 agacgtgccc cgccggcagg gcggtgagcg tcagtccgtt ccactgcagg ggctcgccga
     2701 aaagcatcag ccgcgtcggg cgcgggccgt accgtcgctg atagagtgcc gcagtcgccg
     2761 gcgtgcaata ggccagctcg tgccggccca tgtggtcggt gtgggcgtgc gagatgaatc
     2821 cccgcggctg acggcggcga aaatcgacgg cgagatcgat gctggtgatc ttcagcccgc
     2881 cgtcgaagtg gaacatcggg gctccgctat agggtgcacg agtccgctgc ggccgaaatg
     2941 caccaagcga ttcggaacat ggtgcatttc gctgcgctta tgcaccccta cgacttctca
     3001 gccgtggcga cggctgtttc gccgctgacg cccgccagca acccgtcgag cgccgtcgaa
     3061 gcgcgtgtga tcgtcgcttc gcgcgtttgc cctttggacg actccggatc gggagcgacc
     3121 gtcaccggtc catcgtatcc cagctcttcc agaatgccga gcagggcagc acagtcgatc
     3181 gcgccgcctt cgcccggcat cagcctctgc tgcgaggtga ttgccgccag gtcggcatcc
     3241 gccggtagat cggcgaggcg cacgctcaga atctgctcgg ccttcagcgt gcgaactttt
     3301 tccaggtcgc cgccgccgac aaaccagctc caggtgtcga gcaccagacc cacgttttcg
     3361 cgctgcacgg agttgagaag cgtgagcaac ggctcgacct gatgaatgaa ttcgaagccg
     3421 ccgtcggcac gatctgccgg cgcggccagg agtaccaacc cgagtttgac attcccgggt
     3481 gccagggcgt cggccaactt ctggagacgc tctgtgtgaa atttgaaatt ctcgtgaaag
     3541 ggacgctgat cgcacgtcgg gcgaatgctc gtgaagcaac ggtcggcatg gagcgtggac
     3601 gccacctcca agagcaacgg caactgcgcc agatcggccg cgaattcctg ttcttcgccg
     3661 gcccagcgaa ccggcagttc aaagccgcca atggccatgt gggcactgga caaatatcga
     3721 caagcctgcg gcactccggt tgtttgggcc cgcttcgtca gctccgtaat gtcgatttcc
     3781 aggcccttaa agcgatgcgt cagagcgatc tcgacaagtt cgctctgccg tcctataact
     3841 ccaatggcag aaggacttag gcacttatac atacgctcct cctttgttcc ggttaacggc
     3901 cgcttctgcg cccatagcag caagccgtct gataagattc cgcccattat cgccgctccg
     3961 ccggatggat caaccgggtg accctccggg tgagtgggca gacgggaggc agaacgtata
     4021 gtttgcggga aatcccatcg cctgcgattc gagagcacgc caatttgtcg cgaggtcctg
     4081 ctagcatgcc gttttcttgg tggcaaatcc tgatcctcgc ggtgctgcag ggggtggcag
     4141 agttcctgcc gatcagctcc agcggccatc tggtcgtcct cgcgccgctg ctgtttggca
     4201 ccagcgaagc cccgaccgac atgactgccg ttaccgtcgc gctgcacatc ggcacgctcg
     4261 gctcgatcct tgtctattac aaccgtcgca tcgcacggct gctgggcgaa gatcgcggca
     4321 cgctgggatt aatcgttatc ggaagcattc ccgcggcggt ggtcggtttg ctcatcgaac
     4381 ttggcaacga gcagattatc gagcagccgc tcttggccgg gtgcatgctt ccggtgacgg
     4441 gcggcgtgct gttgtggatg tcgcggcgac ccgcggggac gacggaatat cgcgatatga
     4501 gctggcgcag cgcgctcgtt gtcggcttga gtcaggccgc ggccattctt cccggcctct
     4561 cgcggagcgg cctgacgatc gccgcgggag tcggcacggg actgtcacgc gcttcagccg
     4621 ctgcgttctc attcctgctg gcaattccgg tgatcggcgg agccggattg ctcaagctgc
     4681 tactgacgga tcagcagccc gcgacgccag tcagcttgct attcatgggg ggcggcgtgg
     4741 cctttatcgt cggcatcgcg gccctgtggt gcctcgaccg gatgctgcag cgcgggcggc
     4801 tgcactggtt cgcctggtat tgcattgcgc tgggtttggc cgtgatcgcc tggcagctct
     4861 cagccatttt caatccatag ccgcaggcgt tccggctcga ggcgcacaga atgcccggaa
     4921 agggcttcgc ggccgatccg cgagccccaa tcttccgcct ccggcggcag gaccagcgcc
     4981 gcaacatcgc gcagcacgca gccgctgaac cgcaggtacg gaatggtgac gcgacgggcg
     5041 acgacgctgc gtccggcgga tagaaaaacg gtctcgcgcg gagcgtcctc tggaacagaa
     5101 atacccgccg cctccgcggc tgtggcggag accatcgttg tctgcgtccc ggaatcgacc
     5161 caagtgaagg tcagcggcga gagttcgtca acgagccctg tgaggcgatg ttgccccgcc
     5221 tgcaaatgga gcgggtgcca gggcgtgaac accagtcgct cgtactcgcc gaggtttcgc
     5281 atgtcgcccc ggtagctccg ctgcgggccg agacgctgcc cttggcccac tctgccaaga
     5341 gcactctgca cttcttgacg ctgaccaagc tccatgtact gatcgagcag ctttggcaac
     5401 gcacggcgca ctcgcaccgt cgccgcggcg aggtcgttcc gctcggctgt ccactgcacg
     5461 gcggaagtcc gcacttcggc gcgcgaaccg atgcggcccg gttcggtgac agccgcttcg
     5521 agcgcgtcga tctgctgctg caggacgacc cgctgcggat cgttcgacga aagtttcgcg
     5581 accgactgct tgagcgccgc tacgcggccg cggctgtcct gccaggcgag gcgattctgc
     5641 tcgatccgct ggtccagctc gcgctccagc cccacaatcc gctcgcgccg cttgggcaac
     5701 tccgccaggt cctgccgcaa ttccaattcc agcggcagca cccagacacg gtcgagccgg
     5761 gtcaattcat gccgcgccag gatcgcatcc gcggattcgt catcggccgc gggcgcaacg
     5821 tccggcgtgc ctaggagaat cgcagccgcg gcaaacgtgg caagagtgac gacgaggggt
     5881 cgcatgcctg atgataaacc tgccggttcg cgccgggcaa ccacaccgcc gcggatgtgc
     5941 ggctttcgac ggtcctgcgg gcgggcgcgc gccgactgct ttgcagccgc cctccctgcc
     6001 gatcttgtga gcgaatggac tcgcagcggc aggccgcgcg cctactggct tggacgatgg
     6061 atcggcttgt gagcacttgc tgagggtgtg cgatccatgt ggcgagccgt atttctggcg
     6121 attggcatct cgatgtgcat cgcgggcgga gagtgcctcg tcgtcgagaa gttcgtcatc
     6181 ggggccgaga gcccgcctcc tactccgcaa acgcccgcga ccctcttcgg cgccccgccc
     6241 tccggaactg ggcctgcggc acgcgatatc gaaccagccg attggacccc ctggagcctc
     6301 ctctccgccg gagcggttgt cattctctac gccattacgc tgaaccggca gggatagctg
     6361 cgaaccggcc ttcgttcaca gtaagcgcga agcgtaagcg agggagtgcc tcgcttacgt
     6421 ttcaggttgt ccgggattcc ctcgcttacg cttcgggttg gtgtaacgcc cccgtaacgg
     6481 cctcttgctc gcaagtcctt ccccgcgaaa actttccggg cggattgggc gtctaactct
     6541 tatgggcctg gaaccggttc ttgaatcaag cggtcaaatt acccatcttc acaggatcgc
     6601 ggctatgaaa cgcatttctc tcctggcgcc cttggctttc tttcttctgg cggggtctgc
     6661 tttcgggcag ggtgtgctga tcatcatcga tccgccccat ccggctccgc tgccgcggcc
     6721 aatcatccgg ccggtgccca cgcccccgcc gatgtcgtac aagatcaagg agttggacta
     6781 ttacgcccgg gtagtcgatc aagtggcaca agtccaagtc acgcaatcgt ttgtaaacac
     6841 aggcagccag cagatggagg tcagcttcgt cttcccgctc cccta
//
LOCUS       DASUGB010000233         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1693882, whole genome shotgun sequence.
ACCESSION   DASUGB010000233 DASUGB010000000
VERSION     DASUGB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693882"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFV87_04535"
     CDS             complement(<1..1239)
                     /locus_tag="VFV87_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEX5103052.1"
                     /translation="MSEPGKPSPAEGIKEQSRFLRGDVGTELCDGLDHFGKSSESLLK
                     FHGTYQQDDRDERGVGGLDAEGKKKKSYIFMVRSKIPGGKLTSEQLLAQLDLGDEVGN
                     TTLRITSRQGLQLHGVIKSNLKRAIARINEIQLSTLGACGDVNRNVMCCPAPYASNVY
                     RQTQALADQFAAHFAPRTTAYHEIWFTDPATGDKQLVGGAPEDDPTGFQTEPIYGKHY
                     MPRKFKMAIGYPQDNCVDVYANDLGLLAVTEGDEIIGYNLVAGGGMGVTPSAKKTFPA
                     VARRLCYVSPDEVLKVVEAVFKVQRDFGNREDRKLARLKYVIANWGIEAFKAKVEEYY
                     GGPLAEFRVDEDVRGFNDHMGWDEQGDGKLFYGLNVENGRIKDTAEMRLKTALREVCQ
                     TLQPSIRLTAHQSILFGDIRP"
     gene            complement(1414..2373)
                     /locus_tag="VFV87_04540"
     CDS             complement(1414..2373)
                     /locus_tag="VFV87_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103053.1"
                     /translation="MPRLSILIPCVGGAAEFDATLVSVLQHRPDDCEVLVIHTEPYSD
                     PYDLGAEVQFLRSARDAKRTELINEGLAAATGEIVHLLGCGAEATEAWAQPALDRFHD
                     PEVAAVAPLVMDTTGGKLLAAGVRWTLGGSRRIVADQRIKLGGSARLRAGILGPTLEA
                     GFFRRDALQALNGFDTRMDERLADVDLALSLAALDLQTVCEPASRVLLSSTQADACPF
                     GFTSGWSAERLFWRHAAPRGLLASLAFHPLTLIGDALRTRSLAGTLAAGLGRLAAWLE
                     IGSSARYQKRLAVARHRLAETNEPTTIRLAEPAKVAIAKRRAA"
     gene            complement(2543..3799)
                     /gene="pepT"
                     /locus_tag="VFV87_04545"
     CDS             complement(2543..3799)
                     /gene="pepT"
                     /locus_tag="VFV87_04545"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX5103054.1"
                     /translation="MPMVNHSRLLGRFLRYVQIDTTAGEPGGAYPSSPGQLVLGKLLS
                     DELREMGLKDVEHTEHGLVYATVPATVKPQGDQPLPVVAFNAHVDTSPETTGKNVKPH
                     VIRNYSGGDIALAGDSTKVIRVADNPELTTLVGKTLITTDGTTLLGADDKAGVAVIME
                     LAHTLIEHPQLPHGPVRILFTCDEEIGHGIDHVDLEKLGATAAYTLDGSGTGDIDTET
                     FSADLATVTVRGVNIHPSIGKGRMVSAIRAAAAFIDRLPKNGLSPETTEGREGFLHPY
                     TIEGGVAEAKIKILLRDFVTAELAAKAELLRKAAREVEAEIPGCQFAIDVIKQYRNLG
                     DGLAKEPRAVAYVQEAYRRLNREGNLTIIRGGTDGSQLTERGLATPNLSTGEHNPHSP
                     LEWTCLEEMVQAVELLEQLVQVWSGK"
BASE COUNT          735 a         1307 c         1263 g          780 t
ORIGIN      
        1 ggggcggatg tcgccgaaca ggatgctttg atgagcggtg aggcggatcg acggctgcag
       61 cgtctggcag acctcgcgga gggccgtttt cagccgcatt tcggctgtgt ccttgatgcg
      121 gccgttctcg acgttcaggc cataaaacag cttgccgtcg ccctgttcgt cccaccccat
      181 gtggtcgttg aagccgcgga cgtcttcgtc cacacggaat tcggcgagcg gcccgccgta
      241 atactcttcg accttggcct tgaaggcttc gatgccccag ttcgcgatga cgtacttgag
      301 gcgggccagc ttgcggtcct cgcggttgcc gaaatcgcgc tggactttga aaaccgcctc
      361 gacaaccttc agcacttcat ctggcgaaac gtagcacagc cggcgagcga ccgcgggaaa
      421 agtcttcttc gccgagggcg tcacgcccat cccaccgccg gccacgaggt tgtagccgat
      481 aatctcatcg ccttccgtga cggccaagag accgagatcg ttggcataca catcgacgca
      541 gttgtcctgc ggatagccga tggccatctt gaacttccgc ggcatgtagt gcttgccgta
      601 gatcggctcg gtctgaaagc ccgtgggatc gtcctccggc gctccgccga cgagctgctt
      661 gtcgccggtc gccggatcgg tgaaccagat ttcgtgatag gccgtggtgc gcggggcaaa
      721 gtgagcggcg aactggtcgg cgagcgcctg cgtctgccga tagacgttgc tggcatacgg
      781 cgcggggcag cacatgacgt tgcggtttac gtcgccgcac gcgccgagcg tgctgagctg
      841 gatctcgttg atgcgagcga tggcgcgctt gagattgctc ttgatgacgc cgtggagctg
      901 caagccctgc cgcgaagtga tacggagagt cgtgttgccc acttcgtcgc ccagatcaag
      961 ctgcgccagg agctgctcgc tggtcagctt gccgcccgga atcttcgagc ggaccatgaa
     1021 gatgtacgac tttttcttct tgccttccgc atcgaggccg ccgacgccgc gctcgtcgcg
     1081 gtcgtcctgc tgataggtgc cgtggaactt cagcagcgat tcgctcgact tgccgaagtg
     1141 gtccaggccg tcgcacaact cggtcccgac atcgccgcga aggaaccggc tttgctcctt
     1201 gattccctcg gctggacttg gttttcctgg ctcagacatg gcaggcaggc cccgtcatgg
     1261 gcggcaaaag ttcttggcta gcaatgactt cggaattaca atcacgaaac atcatcatgt
     1321 ttagaatgat tgtaaagtat agagtcgcga agtcaagctg ttcgagcccg agagtatagc
     1381 gggactgggc aagatgtcct acgccattgg cgatcaagcg gcccgccgct tggcgattgc
     1441 caccttcgct ggctcggcca gtcgaatcgt cgttggctcg tttgtttcgg caagccgatg
     1501 cctggcgacc gcgagccgtt tttggtagcg ggcggacgag ccgatttcga gccaggcagc
     1561 gaggcgcccg agtccggctg ccagagttcc ggcgagcgac cgcgttctca gggcgtcgcc
     1621 aatcagagtc aacgggtgaa aagccagcga tgccagcaat ccacgagggg ccgcatgccg
     1681 ccagaagagg cgttcggcgc tccagccgct ggtaaagccg aacggacaag catcggcctg
     1741 ggtggacgac aggagaaccc gtgatgcggg ttcgcagacg gtctgcaagt ccaacgcagc
     1801 cagcgacaga gccagatcaa cgtcggccag acgctcgtcc atcctcgtat caaagccgtt
     1861 cagggcctgc agcgcatcgc ggcggaagaa accggcctca agcgtcggcc ccaggattcc
     1921 cgcccgcaac cgggccgagc cgccaagctt gattcgctga tcggccacga tccgccgcga
     1981 gccgccgagc gtccagcgga ctcctgccgc cagcagcttc ccacccgtgg tgtccatcac
     2041 cagcggcgca accgcggcga cttccggatc gtgaaagcgg tccagggcgg gctgggccca
     2101 agcttcggtc gcttcggcgc cgcagcccag caagtgaaca atctctcccg tcgcggcggc
     2161 aagcccttca ttgatcaatt cggtccgctt cgcatcgcga gcagagcgga gaaactggac
     2221 ctccgccccc agatcgtacg gatcgctgta gggctcggtg tgaatcacca gcacctcgca
     2281 gtcatccggc cgatgctgga gcaccgagac gagcgtggcg tcaaactccg ccgcgccccc
     2341 gacgcagggg atcagaatcg aaagccgagg cacgggacct tccttagtcg aacccggcag
     2401 aagctggcgg tgctagcggg aatgagcatc catgcacaag ccgccgtcaa gctattttcg
     2461 gacgccgccg gtgggggtga ttatccaaat tgccgcagcc ctgcgaatcg ggctggcaca
     2521 gattgcccag cttccgcagt cgctacttgc cggaccatac ttgcacgagc tgctcgagca
     2581 gctcgaccgc ctggaccatt tcttcgaggc aagtccattc caaaggtgaa tgcgggttat
     2641 gttcgccggt cgagaggttc ggcgtggcga gcccgcgctc ggtgagctgc gatccatcag
     2701 tgccaccacg gatgatcgtc agattgccct cgcggttgag ccgccggtac gcctcctgca
     2761 catatgccac ggcccgcggc tctttggcca ggccgtcacc cagattgcgg tactgcttga
     2821 tcacgtcgat tgcgaactgg cagccgggaa tttcggcctc gacctcgcgg gctgccttgc
     2881 gaagcagctc ggctttggcg gctaattcgg ctgtcacaaa gtcgcggagc aggatcttga
     2941 tcttcgcctc cgccacgccc ccctcaatcg tataggggtg caaaaagccc tcccgcccct
     3001 cggtcgtctc gggcgagagg ccatttttcg gcagccgatc gatgaacgcg gcagcggccc
     3061 gaatcgcgct gaccatccgc cccttgccga tcgacggatg aatgttgacg ccgcgaacgg
     3121 tcaccgtggc cagatcggcg gaaaaagtct ccgtgtcgat gtcgcccgtg ccgctgccgt
     3181 cgagcgtgta ggcggccgtc gctcccagct tctcgagatc gacgtgatcg atgccgtggc
     3241 cgatctcctc gtcgcaggtg aacaggattc gcaccggtcc atgtggaagc tgcggatgct
     3301 cgatcagcgt gtgggccagc tccatgatga cggccacgcc cgccttgtcg tcggcgccga
     3361 ggagcgtcgt cccgtcggtg gtgatcagcg tcttgccgac gagcgtcgtc aactcgggat
     3421 tgtccgccac acgaatcacc tttgtcgaat cgcccgccag cgcgatgtcg ccgcccgaat
     3481 agttgcgaat gacatgcggc ttgacgttct tgccggtggt ttccggcgac gtatcgacat
     3541 gcgcattaaa agccacgacc ggcaagggct gatcgccctg cggcttgacc gtcgcgggaa
     3601 ccgtcgcata caccaggccg tgctcagtgt gctcgacgtc cttgagcccc atctcgcgca
     3661 attcatccga caagagcttg cccagcacaa gctgcccggg cgagctggga tacgccccgc
     3721 ccggctcgcc ggccgttgta tcaatttgca cgtaacgcag aaaccggccg agaagtcggg
     3781 agtggttgac cattggcatt tcggattttg gatttcggat ttcggattga acgcaggata
     3841 ccagactcgc cgccgcgaat ccgcagtccg aaatcccaaa tccgcaatgg acacccctcg
     3901 cgtttcgcga gccgcgtgcg tgtgctacct tgcctttcat ggccaaagcc gccgtccggt
     3961 acctggtatt cgacatcgaa agcgtcgccg acggcgcgct cgtctcgaag ctccgctatc
     4021 cggccgaatc gctcagcccg gccgacgcgg tgcgccgcta tcgcgatgag ctgctcgcca
     4081 agtac
//
LOCUS       DASUGB010000234         4153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_443641, whole genome shotgun sequence.
ACCESSION   DASUGB010000234 DASUGB010000000
VERSION     DASUGB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443641"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..419
                     /locus_tag="VFV87_04550"
     CDS             192..419
                     /locus_tag="VFV87_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103055.1"
                     /translation="MNSKKRGGAPNVDSVWESHLQADRPCTDRRSKTGPSRLQQVGAE
                     YGPMGEYSNLASTEANCQPTWIAEQCSIVKT"
     gene            complement(1289..1780)
                     /locus_tag="VFV87_04555"
     CDS             complement(1289..1780)
                     /locus_tag="VFV87_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103056.1"
                     /translation="MRGPTAGQLWQRRALLSDHERDTFLRELAERRIQARRDLNLPAD
                     GDLKHYPQAAVDRRAVRDTLVTQGLLEIQPRRRKRSPPEASQRAAASSSPSPVQALVI
                     KNLAPVALDASGAGRIADASTSAPTTTSTALAPELNIEPPGIQPHEEAHSSTNKSSAS
                     GQH"
     gene            complement(1865..2434)
                     /locus_tag="VFV87_04560"
     CDS             complement(1865..2434)
                     /locus_tag="VFV87_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX5103057.1"
                     /translation="MGLALSANRLAVGALVEPRHCRCGDFRDWVRPGDVGGRPACHTP
                     SLPRADRAREGRYAWILAVKDLASRCQLAWLPVEEQTAETVQAVYAQLFAEHGPPLVL
                     KSDNGGPFRDEGTKRLLAEHLVVPLYNPRRRPAYNGGVERSNGQLAGYQEALAAFRGR
                     ATAQPAVSIPTLTCEDAASARNLASELAR"
     gene            complement(2329..3042)
                     /locus_tag="VFV87_04565"
     CDS             complement(2329..3042)
                     /locus_tag="VFV87_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5103058.1"
                     /translation="MVAREVTSADRFVAIAALLLPTVVTLVYFVALDGAAERVQQVAF
                     AIGKGVQFGLPLVWVLGVQKDRLKFVRPDGQGLALGALFGLAVAGLMAAIYLLWLKPG
                     GLMDKPAEEAREKIKSFGVDSVAAFAAMAVFFSAVHSLLEEYYFRWFAFGQLRRWINV
                     PAAIGISSLAFAAHHVLILARYFTWTSELTWLFAAGVAIGGVAWAWLYQRTGSLWGPW
                     LSHAIVDAAIFAIGYDLVM"
     gene            complement(3065..>4153)
                     /locus_tag="VFV87_04570"
     CDS             complement(3065..>4153)
                     /locus_tag="VFV87_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5103059.1"
                     /translation="EGSDVGPALYYTSLDVAPEKLAGSTARIFLGLQIECAQCHDHPF
                     DHWKQKDFWGYAAFFARLRQPDMNNPGMRVTLVDLPEGEVKLPNTDEVVPPKYPGGIS
                     PPADEAGTRRQQLVIWMASRDNPYLPRAGVNRVWWHLFGRGLVEPVDDLGPQNPASHP
                     ELLTELAEYFVNTGFDLRELYRTLANTETYQRTSVGTQDPPPAPELYVHMAVKTLTAE
                     ELYDSVNRVLHRKPQGMMPGVNVASPLLDPQRQAFIAKVQSSGRNAQEYQAGILQALT
                     LLNGADLAEATDPERGPLLGSLEAPFLTDDDRLRTLFLATFSRQPTDEELSLCREQLA
                     KYSDSDRAKAYSDILWALLNSAEFALNH"
BASE COUNT          787 a         1310 c         1279 g          777 t
ORIGIN      
        1 ccaactcccg aatctgatcg gcatccatgc ctgggcctcc gtaactcctt gtggaggccc
       61 cagtttcaac caagctcgaa tccaggacaa taccaaccta gcgctgtagt actaggcctc
      121 tccccattcg gacgtggtgg atgacgcccc gtgacccgca gcgcggaata cgctaaaatg
      181 agcacccccg cgtgaactcc aaaaaacgtg gtggcgctcc gaatgtcgac agtgtctggg
      241 agagccatct tcaggccgat cgtccatgta ccgatcgccg cagcaagacc ggcccatctc
      301 gcctccaaca ggtgggtgcc gaatacggcc caatgggcga atacagcaat ttggccagca
      361 ccgaggccaa ctgccaacca acttggatcg ccgagcaatg ctcgatcgtt aaaacctagc
      421 agcccagtcg cttcagactt caaagtagaa gtgaagcctg ggccaagcag tacgatgcaa
      481 tcgacaacaa tactcagtgc cacaaacatc atactggcgt acaaatactg gtgtccgcgc
      541 gcggcacatc tagtagtgtt gtccgatgtg taagtggaga ccccctgact cattgtagta
      601 attcacgcta ttcgcagtct tcttcttcaa ctgtcttagt cggcgtgccc ggaagtgcac
      661 aatttgggac tctaggcatt tgatctccaa tcggtcgata atccttgcct gctctgggaa
      721 caaagtcctc gctcttatta tcgtgactct cgtggagggc aactagcttg tcaatgtccc
      781 gatttgcata atatagtgcg aacttgatta acgctcggca atccctcagc gacgagaaga
      841 tgcgacgttc tacttggtcc gagaagacgt cgccaagctc ccttgcaagg tctaagaaag
      901 cctgcaaatg aagctgctca tgaccatagc tccctgggac agtgtagcga ccgaagatgt
      961 gctccttcgc aatccgtatc tggaagtatg ccgtgaccac acaccgtggt tggtatagac
     1021 gtgttggaca ctgccgactg ccaagccaga tgcattggca ggcacaagtc gcttggtact
     1081 taacaatttt cgtgtcggcg cgtacatgtg gtgcgagccc gttaggatcg ggtttcggtc
     1141 gcgatcgccc cggaaacgta tccgtgggat cttcggcgag aacgttcgac cccacattgc
     1201 accacggtgc attcttggcc tgcgttggcg aaagatcgta cgtgacaagg ccactcgggt
     1261 ccactgcgtt tacaggtgtg ccccatcgct aatgttgtcc gctagctgag gatttattgg
     1321 tggaggaatg ggcctcctcg tggggttgga ttcccggcgg ctcgatgttg agctccgggg
     1381 ccagcgcggt gctcgtcgta gtgggagcgc tcgtgctggc gtccgctatt ctacccgcgc
     1441 cgctagcgtc aagcgccaca ggcgcgaggt tttttatgac gagtgcctgc accgggcttg
     1501 gtgaagaact tgctgcggcg cgctgtgatg cttccggcgg gctgcgcttt cgccgccgcg
     1561 gttgaatctc cagcaggcct tgggtgacga gcgtgtcgcg caccgcgcgg cgatcgacgg
     1621 ccgcttgcgg gtaatgtttc agatcgccgt cggccggcag gttcagatcg cggcgcgcct
     1681 ggatgcggcg ctcggcgagt tccctgagga acgtgtcacg ttcatggtcc gagagcagag
     1741 cgcgtcgctg ccagagttgg ccggccgtcg gtcccctcac gggcgttgaa aaaggggaca
     1801 ggcacgtcgc cgcaaccggt gttcgcgggt cgaaaaattg ctcgctccga gccagtcccc
     1861 tttttcaacg ggccagttcg ctggccaggt tccgggcgct ggcggcgtcc tcgcaggtca
     1921 gcgtagggat actgacggcg ggctgcgcgg tggcgcggcc ccggaaggcg gccaacgctt
     1981 cctgatagcc ggccagttgc ccattggagc gctccacgcc accgttgtaa gccggtcggc
     2041 ggcggggatt gtagagtggc acgaccagat gctcggccaa cagccgcttg gttccctcgt
     2101 cccggaacgg cccgccgttg tcgctcttga ggaccagggg tggaccatgc tcggcgaaga
     2161 gctgggcata gaccgcctgc acggtttcgg ccgtttgctc ctctaccggg agccaggcca
     2221 attggcagcg gctggccagg tccttcaccg ccaggatcca ggcatagcgc ccctcgcggg
     2281 ctcgatcggc tcgcggcaga gagggggtat ggcaggcggg acggccgcct acatcaccag
     2341 gtcgtaccca atcgcgaaaa tcgccgcatc gacaatggcg tggctcaacc agggccccca
     2401 cagcgagccg gttcgctgat agagccaggc ccacgcgacg ccgccgatgg ccacacccgc
     2461 ggcaaagagc cacgtcagtt cggacgtcca ggtgaaatag cgggccagaa tcagcacatg
     2521 atgcgccgcg aaagccaggc tcgaaatacc aattgccgcc ggcacgttga tccaccgccg
     2581 aagctggccg aaggcgaacc agcggaagta atactcctcc aatagcgaat gaactgccga
     2641 gaagaagacc gccatcgccg cgaacgccgc gactgaatcc acgccgaacg acttgatctt
     2701 ctcccgtgcc tcttcagccg gcttgtccat caggccgccc ggcttcagcc acagcaggta
     2761 gatcgccgcc atcagcccgg caaccgccag gccgaacagc gcgcccagcg ccaacccctg
     2821 cccatccggc cggacgaact tcagccggtc cttctgcacg cccagcaccc acaccagcgg
     2881 taagccgaac tgcaccccct tgccgatcgc aaacgccacc tgctgcaccc gctctgccgc
     2941 cccgtccaag gcgacgaaat agaccagcgt caccaccgtc ggcagcagca gcgcggcaat
     3001 cgcgacgaac cggtcggcgg acgtgacttc cctggccacc atggcgacca gtataaacgg
     3061 ccggctaatg attcagcgcg aactcggcgc tgttaagcag cgcccagagg atgtcgctgt
     3121 aggccttggc ccggtcgctg tccgagtatt ttgcgagctg ctcgcggcag agggagagct
     3181 cttcgtccgt gggctgccgg ctgaaggtgg cgaggaacag agtcctgagg cggtcgtcgt
     3241 cggtgaggaa gggcgcttcg agcgagccaa gcagcggacc gcgctcggga tcggtcgctt
     3301 cggccaggtc cgcgccgttg agcagcgtga gcgcctgcag aatgcccgcc tggtattcct
     3361 gcgcgttgcg gccggaggat tgcaccttgg caatgaacgc ctgccgctgc gggtcgagga
     3421 gcgggctggc gacattcacg cccggcatca tcccctgcgg cttgcggtgg agtacgcggt
     3481 tgaccgaatc gtacagctct tctgcggtca gcgtcttcac ggccatgtgg acgtacagct
     3541 ccggcgcggg cggcggatcc tgtgtcccaa cgctcgttcg ctggtatgtt tccgtgttgg
     3601 ccagcgtgcg atacagctcg cgcaaatcga agccagtgtt cacgaagtac tcggccagct
     3661 cagtcagcag ctctggatgg ctggccggat tctgcgggcc caggtcatcg accggctcga
     3721 ccagcccgcg gccgaagagg tgccaccaga cgcggttgac gccggcccgc ggcaaatacg
     3781 ggttgtcgcg cgaggccatc cagatcacga gctgctggcg gcgcgtcccc gcttcgtccg
     3841 ccggcggcga aatgccgccc ggatatttcg gcggcacgac ttcgtctgta ttcggcagct
     3901 ttacctcgcc ctcgggcaga tcgacgaggg tcacccgcat tcccgggttg ttcatatccg
     3961 gctgccgcag gcgggcgaag aacgcggcat agccccagaa atccttctgc ttccagtggt
     4021 cgaacggatg gtcgtggcac tgggcgcatt cgatctgcag tccgagaaaa atgcgggccg
     4081 tgctgccggc aagcttttcg ggggccacgt cgagcgacgt gtaatagagc gccgggccga
     4141 cgtcgctccc ctc
//
LOCUS       DASUGB010000235         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_165258, whole genome shotgun sequence.
ACCESSION   DASUGB010000235 DASUGB010000000
VERSION     DASUGB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165258"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /gene="dnaN"
                     /locus_tag="VFV87_04575"
     CDS             <1..1055
                     /gene="dnaN"
                     /locus_tag="VFV87_04575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5103060.1"
                     /translation="GRSPKEILTNIKLDVDKEGATFSATDMEVGIRVKIEGLDVDAPG
                     SAILPVVNFGAILRENTDEKLRIDASSKGTVVRGERSEFKLPAGDPDEFPSIAKFEET
                     KYHVIPARLFKEIIARTEFATDMESSRYALGGVLIELEANKITAVGTDGRRLAKMEGP
                     AEAVAGHKTGDSMTIIRSPSMRLIGRALSESDTEVHLAARGNDVLLRTPRSTFYSRLV
                     EGRFPRWRDVFPARRDAARIDMTVGPLLSALRQASVVLSQESRGIDFTLGDGTLTLSG
                     ATAEAGQSRVEMPIAYDGPQIIVSLDHRFVLEFLKVLSPEQNITLDIENGETATVFHA
                     DDDAYGYVVMPLSRDR"
     gene            1106..1474
                     /locus_tag="VFV87_04580"
     CDS             1106..1474
                     /locus_tag="VFV87_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5103061.1"
                     /translation="MSKRFIDDDERDLAADYRRKQIRLPPPKAVGNVLSQLLAKRGYA
                     QIQTAATCDAAWREAVGEKLAADTRPGCVKRGVLEVLVRNSTVLQELAFVKAKAVKTL
                     TQLVPEQQIRDVRFRVGAID"
     gene            1657..>2069
                     /locus_tag="VFV87_04585"
     CDS             1657..>2069
                     /locus_tag="VFV87_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5103062.1"
                     /translation="MSAELTASESLATLPLPDDYDAEKFKKLSDREHVRLRYGMYIGD
                     SSVAGLHHLVYELVNNSIDEVMAGFAQHIWVTINNDGSCTVEDDGRGIPIERHEQTSE
                     EKGRDVPTLEAVMTNLNMGAKFDKLSYKTSGGLHG"
BASE COUNT          421 a          694 c          630 g          324 t
ORIGIN      
        1 ccggccgcag cccgaaggaa atcctgacga acatcaagct cgacgtcgat aaggagggcg
       61 ccacgttcag cgccactgat atggaggtcg gcatccgcgt caagatcgaa gggctcgacg
      121 tcgatgcgcc cggcagtgcg attttgcccg tcgtcaattt cggggcgatc ctccgcgaga
      181 acaccgacga gaagctgcgg atcgacgcca gcagcaaagg gaccgtcgtc cgcggcgagc
      241 gaagcgagtt caagctcccc gccggcgatc ctgatgaatt tccgagcatc gccaagttcg
      301 aggagacgaa gtaccacgtc atcccggccc gcctgttcaa agaaatcatc gcccgcaccg
      361 agttcgcgac cgatatggaa agcagccgct atgccctggg cggcgtgctg atcgagctgg
      421 aggccaataa gatcaccgcg gtcggcaccg atggccgccg cctggcgaag atggaaggcc
      481 ccgccgaagc ggtcgccggc cacaaaacgg gcgattcgat gacgatcatc cgctcgccgt
      541 cgatgcggct gatcggccgg gcattatcgg aatccgatac cgaggtgcat ctggcggccc
      601 gaggcaacga cgtcctgctt cgcacgccgc gatcgacgtt ctattcccgc ctggttgaag
      661 gccgcttccc gcgatggcgg gatgttttcc cggcccgccg cgacgcggcc cgcatcgaca
      721 tgaccgtcgg cccattgctc tccgccctgc ggcaggcctc ggtcgtcctc agccaggaaa
      781 gccgcggcat cgatttcacg ctcggcgatg gcacgctcac cctgtcgggc gctacggccg
      841 aagccggcca atcgcgggtc gaaatgccga ttgcctacga cggtccgcag atcatcgtct
      901 cgctcgacca tcgcttcgtg ctcgagttcc tcaaggtgct gagccccgag caaaacatca
      961 cgctggacat cgagaacggc gaaacggcca cggtcttcca cgccgacgac gacgcgtacg
     1021 gctacgtcgt catgccgctg tcgcgagatc ggtaacgtgg ggcaggctgc cagcctgcca
     1081 ggcttcaagg aggaacgtgc aaccaatgag caagcgattc atcgatgacg acgagcgcga
     1141 cctggcggcg gactaccgcc gtaagcagat tcgcctgccg ccgcccaaag cggtgggcaa
     1201 cgtgctgagc caattgctgg ccaagcgcgg ctatgcccag atccagaccg ccgccacgtg
     1261 cgacgccgcc tggcgcgagg cggtcggcga aaagctcgcc gccgatacgc ggcccggctg
     1321 cgtgaagcgg ggcgtcctgg aggtcctggt ccgcaattcg accgtcctgc aggagctcgc
     1381 cttcgtcaaa gccaaagccg tgaaaaccct gacccaactc gttcccgagc agcagattcg
     1441 cgacgtgcgt ttccgcgtgg gagcgattga ttaagtgggg caggcattcc tgcctgccag
     1501 aacgcctcgt gcccaagctc tgcttgggca cgccagtcct cgaagctccg cttcgcgggg
     1561 cctggaagca gagcttccag acgtgcgttc ccaagcggag cttgggaacg aggatacgtg
     1621 aaatatccga ccgacagaac gaacccgaaa gcacccatgt ccgcagaact caccgcttcc
     1681 gaaagccttg ccaccttgcc gctccctgac gactacgacg ccgagaagtt caagaagctc
     1741 tctgaccgcg agcacgtccg cctgcgctat ggcatgtaca ttggcgacag ctccgtcgcc
     1801 ggcctgcacc acctggtcta tgagctggtc aacaactcga tcgacgaagt gatggccggc
     1861 tttgcccagc acatctgggt gacgatcaac aacgacggct cctgcaccgt cgaggacgac
     1921 ggccgcggca tccccatcga gcggcacgag caaacgtccg aggaaaaggg gcgcgacgtc
     1981 cccacgctcg aagccgtcat gaccaacctc aacatggggg ccaagttcga caagctcagc
     2041 tacaagacct ccggcggcct gcacggcat
//
LOCUS       DASUGB010000236         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1138072, whole genome shotgun sequence.
ACCESSION   DASUGB010000236 DASUGB010000000
VERSION     DASUGB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138072"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFV87_04590"
     CDS             complement(<1..518)
                     /locus_tag="VFV87_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX5103063.1"
                     /translation="MRGVRTRLKDKAGRIRQFGGDYLAYALIDAVVDSFYPVLDTYAE
                     RLDDLEENVLDRPTRATMDGLHQVKADLLLIRRAIWPQREAVGHLMRETTPQITDATR
                     VYLRDCYDHVVQIVDLLETYRELTADLRDLYMSSISNRINETMRVLTIMSTLFIPLTF
                     IAGIYGMNFDTSS"
     gene            559..795
                     /locus_tag="VFV87_04595"
     CDS             559..795
                     /locus_tag="VFV87_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103064.1"
                     /translation="MRPNENAELFGSSPLVAVDHAEHDKQDVAIFLDLGPLMEVNDVL
                     HRQAMQSEQLAELLDQLRVPQPFDVDPGDRVVRQ"
     gene            complement(975..2354)
                     /locus_tag="VFV87_04600"
     CDS             complement(975..2354)
                     /locus_tag="VFV87_04600"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5103065.1"
                     /translation="MSTRRCVSGRSFCAVALVLALPAVGWGQLSGRTFRSNIPTRVLP
                     QQDLTAEQPEAGVVPAAASQPLPTDGEVLFPREPAPLSTPAAQTEGAWHAGRSTGLKP
                     TGAGISSKAGPKPKSKAPSIVEKTGAEGDPSSKKAGPTDAEQVPEGFTGLFSEEVQDV
                     AQRVLFVWKYPFFKTKDFDLSLQVIIGGLFLLWLGNKLARRLSAWLGDTVLRRVGLPA
                     ATAAPLQKMSFYVLFGAFAVFSLQLVGVPMTVFTFFGGALAIGIGFGSQNVMNNFISG
                     LILLIERPIRVGDVIQIDSHSGKVSEIGARSTRITTGSNLEVIIPNSTFLQGNVVNWT
                     LSDDIISSKVAVGVAYGSPAREVARLLRQAAETHEQILKTPAPGVSFTEFAADSMQFE
                     LSFWLKMSQADRGKVESDLRFKIDELFAGRGIVIAYPQRDVHLNLLRPVEVRLSEPTV
                     APRELRGAA"
BASE COUNT          538 a          842 c          888 g          557 t
ORIGIN      
        1 gagctggtgt cgaagttcat gccgtagatg ccagcgatga acgtcaacgg gatgaagagc
       61 gtggacatga tcgtgagtac gcgcatcgtc tcgttgatgc gattgctgat ggaggacatg
      121 tagagatcgc gaaggtcggc agtcagctcg cggtaggttt ccagcaaatc gacaatctgc
      181 acgacatggt cgtagcaatc gcgaaggtag acgcgggtgg cgtcggtgat ttggggcgtc
      241 gtttcacgca tcaggtgccc cactgcttcc cgctgcggcc agatcgcccg ccggatgagg
      301 agcaagtcgg ccttgacctg gtgcagcccg tccatcgtcg cccgcgtggg acggtcgaga
      361 acgttctctt cgagatcgtc cagccgctcg gcataggtat cgagcaccgg gtaaaacgaa
      421 tcgacgacgg cgtcgatcag ggcgtaggcc aggtagtcgc cgccgaactg gcggattcgg
      481 ccggctttgt ccttgagacg agtgcggacc cccctcaaac gggtctccgg gcgttccttc
      541 ctgaaacgtg aggacgaagt gcggcccaat gaaaatgctg agctgttcgg ttccagcccg
      601 ttggtcgcag ttgatcatgc ggagcacgac aaacaggatg tcgccatatt cctcgacctt
      661 ggcccgttga tggaggttaa cgacgtcctc catcgccagg cgatgcagtc cgaacagctc
      721 gccgaattgc tcgatcagct tcgcgtcccc cagcccttcg acgttgatcc aggtgaccga
      781 gtagtccgcc agtaactgcg gaatcttttt cagatcggtc aatctttctt cgtggacttt
      841 gtcgctgctg taggctagga cgtggacttc cggctggggc tgggcgggat cgacgacgat
      901 ggtgccgggg gacgagccgg ggtgcgttcg ccgctggatg cggaggtcgc gggctttgtt
      961 cttcttgcgg cggctcacgc ggctcctcgc agttcgcgcg gggccacagt cggctcgctc
     1021 aggcggactt ccaccgggcg gaggagattg agatggacgt cccgctgcgg ataggcgatg
     1081 acgatgcccc ggccggcaaa caactcgtcg attttgaaac gcaggtcgct ctccactttg
     1141 cctcgatccg cctggctcat cttcagccag aagctcagct cgaattgcat cgagtcggcg
     1201 gcgaattctg tgaaggagac gccgggagcg ggagttttca gaatctgctc gtgcgtttcc
     1261 gccgcttgcc tgaggagccg ggcgacctcc cgggctgggc tgccataagc gacgccgacc
     1321 gcgactttgc tgctgatgat gtcgtcagaa agggtccagt tgacgacgtt cccttgcagg
     1381 aaggtgctat tgggaatgat gacttccaga ttcgagccag tcgtgatccg cgtgctgcgg
     1441 gcgccgatct cgctcacctt gccgctgtgg ctgtcgattt gaatcacgtc cccgacgcga
     1501 atcggccgct cgatgagcag gatcaggccg ctgatgaaat tgttcatcac attctggctg
     1561 ccaaagccga tgccgatggc gagggccccg ccaaaaaacg tgaagaccgt catcggcacg
     1621 ccgacgagtt gcagcgaaaa gactgcaaac gccccgaaaa gcacatagaa cgacatcttc
     1681 tgcagcggag cagcagtggc ggcggggagt ccgacgcggc gcaggaccgt gtcgccgagc
     1741 caggcgctga ggcggcgcgc cagtttgttc cccagccaga gcagaaacag gccgccgatg
     1801 atgacctgca gcgaaaggtc aaaatccttg gtcttgaaga atggatactt ccaaacgaag
     1861 agcacgcgct gggctacgtc ctgaacttcc tccgaaaata gtccggtgaa gccctccggc
     1921 acttgttccg cgtcggtagg gcccgctttt ttggaacttg ggtctccttc ggccccagtt
     1981 ttttccacga tggatggtgc tttcgacttg ggcttgggac ccgccttgga cgagatgccg
     2041 gcccccgtcg gcttgagccc agtggagcga ccggcatgcc aggccccctc cgtctgggca
     2101 gcgggggtag agagcggagc gggctcgcga ggaaacagca cttcgccgtc ggtcggcaaa
     2161 ggctgcgaag ccgcagcggg gacgaccccc gcctccggtt gctctgccgt caggtcctgc
     2221 tgcggaagga cgcgggtggg aatgttggag cgaaaagtcc gcccgctgag ctgaccccaa
     2281 ccgacggccg gcagagccag caccagtgcc actgcgcaga aggaccgacc agagacgcaa
     2341 cgacgagtac tcacgccagc ctccttgcac gctcggggta tctgtcctgg gtccgctgcc
     2401 cgaattcact ccccggcgcg gattcggcag aaaatcgccc ccatagttag cacaaaccac
     2461 cccactgcgc aaagtgcgac ttgagcgata gcggatttgc tcccctagcg tggtgctcgt
     2521 ttttcccgat tctgagctgt gtcacgaggc accagagctt gtcgcaaatc cgcacaattg
     2581 ctactcttac atgcttcccc tcggccgaac ctaggtgctg tccgcgagtt gtggctgccg
     2641 gcctcagggg agcacctcta acccctgctt cgcgctgaaa aactacctcg tgtcccacaa
     2701 tcgggaccgc tgccggcgct ggagcgtaag gtctgctacc gcttatggtt aatcgcacgc
     2761 tgatccgtga tctggagaac gaccccgata tccagtcgat gtatgaaacc gagatcgaag
     2821 gtttc
//
LOCUS       DASUGB010000237         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1694248, whole genome shotgun sequence.
ACCESSION   DASUGB010000237 DASUGB010000000
VERSION     DASUGB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694248"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..900
                     /gene="cmk"
                     /locus_tag="VFV87_04605"
     CDS             253..900
                     /gene="cmk"
                     /locus_tag="VFV87_04605"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5103066.1"
                     /translation="MIVAIDGPAGAGKSSIARRLAERLGFQFLDTGAMYRAVALAALR
                     QGLGPDDGEAIARLAETLSIDMRHEKTFLNGEDVSAAIRTSEVSAAVYLAADNVAVRR
                     RLVELQRQIADDRDTVTEGRDQGTVAFPHAECKIFLTASPEVRARRRYEELISRGEPA
                     ALEEVLAQQQDRDRRDAARPVGALLKAADAVEVITDGLTPEEVVERLERIVRGYR"
     gene            991..1662
                     /locus_tag="VFV87_04610"
     CDS             991..1662
                     /locus_tag="VFV87_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5103067.1"
                     /translation="MNLEPASRGSPRPRTLFQRVGYDVLRVMARFVAVWLFGLRVQGR
                     EHWPQSGGGLICSNHQSHFDPPLVGLTCSRRMNYLARDTLFNVPGLKQLIQFLDAIPI
                     DREGGGLAGLKETLRRLKAGELVLIFPEGTRTHDGEVAPLKPGFIAVARRSRVPLIPV
                     AIDGAFQAWPRTSKLPRLSRLAVVIGEPIGPEQVSELADDDLLAELEQRILNCHAAAR
                     ELRAK"
     gene            complement(1748..2383)
                     /locus_tag="VFV87_04615"
     CDS             complement(1748..2383)
                     /locus_tag="VFV87_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX5103068.1"
                     /translation="MNDALGILEVTGLTPAMVALDTMDKTASIRVLQTESNDFYGVVL
                     KVTGSVANVKAAIAAGKSAADQMGGKPVSAVINKPDERAWPALESPVEVSPLIQQPIV
                     KTPSYEIISTSREGKSVAENISALGFIETQGFTAVFEAIDSACKAANVEVVGKEKLGG
                     GYVTVVIRGDVAAVKAAVEAGVAKVNGLGKLIAGHVIARPSAAVLSLLPKA"
     gene            2531..>3041
                     /locus_tag="VFV87_04620"
     CDS             2531..>3041
                     /locus_tag="VFV87_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5103069.1"
                     /translation="MSKKHKKRDAKPDSKGTYAFVSLGCPKNTVDSERMLGLLKLDGY
                     DLVPQPEGADFVVINTCGFIERARAESLGAIDEMLALKEAGKTKGVIVSGCLAERQQE
                     DLLDQRPGIDHLVGVFGREEITRVADRLVGHIEEQRTVFRPAPIRALSDRDRLRITPR
                     HFAYLKISEG"
BASE COUNT          539 a          924 c         1038 g          540 t
ORIGIN      
        1 gcatgacgtt tctccgcaga ggaagtgcta aagggctatg gtgagatcag catgcatgtt
       61 ctgtggccgc cagacaagaa ggcggacggg gcctgggcca cggacaggtt gtccgcacgg
      121 acagcgtcga tacccgtgag acggcaccaa actccggccg ctgcaaacga tatacccggt
      181 tttggcagta agtcgctggc aattcgatcg cgggccctca ctcccccgcg gatgaggagc
      241 taaattggac ggatgatcgt ggccatcgat ggtcccgcag gcgcgggaaa gagcagcatt
      301 gcccgccgac tggcggaacg gctggggttc cagtttctcg atacgggagc gatgtaccgg
      361 gcggtcgccc tggcggcctt gcggcagggg ctggggccgg acgacggaga agcgatcgcc
      421 cggctggcag agacgctgtc gatcgacatg cggcatgaga agacgttcct gaacggcgag
      481 gacgtttcgg ccgcaatccg caccagcgaa gtctccgcgg ccgtctatct ggcagcagac
      541 aacgtggccg tccgccggcg gctggtcgaa ttgcagcggc agatcgccga cgatcgtgat
      601 acggtgaccg agggccgcga ccaggggacc gttgccttcc cgcacgccga gtgcaaaatc
      661 tttctgaccg cttcgcccga agtccgcgcc cgccgccgct acgaagagtt gatctcccgc
      721 ggcgagccgg ccgcccttga agaagttctc gcccagcagc aagaccgcga ccgccgcgac
      781 gccgcccgcc ccgtcggcgc cctcctcaaa gcggccgatg cggtcgaagt cattaccgac
      841 ggcctgacgc cggaggaagt cgtcgagcgg ctggaacgca ttgtgcgagg ttatagatga
      901 cgggttatag attgtcggaa atcgatcatc gagcgctgta agattgcacg ttactggcgt
      961 gcgttgacct tcaactttga acgttgaact ttgaaccttg aacccgcctc ccgcggatcg
     1021 ccgcgcccgc gaacgctgtt ccagcgcgtc ggctatgacg tgctgcgcgt gatggcgcgg
     1081 tttgtggcag tgtggctgtt cgggttgcgc gtgcaggggc gggagcattg gccccagagc
     1141 ggcggcgggt tgatctgctc gaaccatcag agccattttg atccgccgct ggtcgggctg
     1201 acgtgttcgc ggcggatgaa ctacctggcg cgggacacgc tgttcaatgt gccgggattg
     1261 aagcagctca ttcagttcct ggacgcgatt ccgatcgacc gcgaaggggg cggcctggcg
     1321 gggctcaagg agacgctgcg gcgattgaag gcgggggagt tggtgctgat ctttcccgag
     1381 gggacccgca cgcacgacgg cgaggtcgcg ccgctcaagc cgggattcat cgccgtcgcc
     1441 cgccgcagcc gcgtgccgct gattccggtc gccatcgacg gggcctttca agcctggccg
     1501 cggacgagca agctgccccg cctctcgcgg ctggcggtcg tcatcggcga gccaatcggc
     1561 cccgagcaag tatccgagct ggccgatgat gatctgctgg ccgagctgga gcagaggatt
     1621 ctgaattgcc acgccgcggc gcgggaatta cgcgccaaat agccgggacc aaacctggtc
     1681 ccgaagtgcc cgatggaagg caactcgcag ttctccggga ccaagtgtgg tcccggctag
     1741 gaacacttta cgccttcggc agcaggctga ggacggcggc gctggggcgg gcgatgacgt
     1801 ggccggcgat gagcttgccg aggccgttca ccttggcgac gcccgcttcg acggcggcct
     1861 tcacggcggc cacgtcgccg cggatgacga ccgtcacgta gccgccgccg agcttttcct
     1921 tgccgacgac ttcgacgttg gccgccttgc aggcgctgtc gatggcctcg aacacagcgg
     1981 tgaagccttg cgtttcaata aagccgagtg cggagatatt ttctgccacg gatttaccct
     2041 cgcgggaggt gctgatgatt tcgtagcttg gcgttttgac gatcggctgc tggatgagcg
     2101 ggctgacttc gaccgggctt tcgagcgcgg gccacgcgcg ctcgtccggc ttgttgatca
     2161 cggcgcttac cggcttgccc cccatctggt cggcggcgga cttgccggcg gcgatggcgg
     2221 ctttgacgtt cgccacggag ccggtgacct tgaggacgac gccgtagaag tcgttcgact
     2281 cggtctggag aacgcggatg ctcgcggtct tgtccatcgt gtcgagcgcg accatcgccg
     2341 gcgtcaggcc ggtcacttcg aggatgccga gagcgtcgtt cacggggagt aaaaccgagt
     2401 agggtgggct gtgcccacca tatgattcgg tgggcacagc ccaccctaca cgctgcacga
     2461 gtatagcttg cccctcgcca ggcgagcgta agcgccatag actgaatgac ttacgaaagc
     2521 gagagccacg ttgtccaaga agcacaagaa acgcgacgca aagcccgaca gcaaagggac
     2581 ttacgccttt gtcagcctgg gctgcccgaa gaatacggtc gatagcgagc ggatgctcgg
     2641 cctcttgaag ctcgacggct atgacctggt gccgcagccg gagggagcgg atttcgttgt
     2701 catcaacacg tgcggcttca tcgagcgggc gcgggcggag tcgctgggcg cgatcgacga
     2761 gatgctggcc ctcaaagagg cgggcaaaac gaagggagtg atcgtcagcg gttgcctggc
     2821 cgagcggcag caggaagact tgctggacca gcggcccggc atcgatcatc tggtcggtgt
     2881 tttcggccgc gaggagatta cccgcgtcgc cgaccggctc gtcggccaca tagaagagca
     2941 gcggacggtc tttcgccccg cgccgattcg cgcgctctcc gatcgcgacc gcctgcggat
     3001 cacgccgcgg cacttcgcgt atctgaagat ttccgaaggc t
//
LOCUS       DASUGB010000238         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1000324, whole genome shotgun sequence.
ACCESSION   DASUGB010000238 DASUGB010000000
VERSION     DASUGB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000324"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..>3022)
                     /locus_tag="VFV87_04625"
     CDS             complement(20..>3022)
                     /locus_tag="VFV87_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5103070.1"
                     /translation="VNPNNPYGFGGAMPMGIVVFSGRGARQPSAPSPVSREEFIEKWR
                     EQGWRPAGQLLFDGGKIFIKAPDQLVAFSTTAHDNRPIWKSVWQNQYELDGRSQAMAM
                     MAMNYGYNFQMQDGKPRSPAEVLLFGDRVHQSMSIADGVIYSIEGKKVSATGEAQQKP
                     AVARGFQWGVIPRRTRGNWLTAYSTAGGKVRWSRTASDEDKEGNSDVGFLAAPVPCGS
                     LLLAPVTDGGTIWLFGLDREDGHTVWKSYLCDEPQGGANPWAPITIAVDGREAYLSCG
                     CGVMFAVDAVGGTVRWAIRYQRDGKPNNMMRQMYGNQGTGLLDLEGWDDDVVIPYGKA
                     VVILASDCDHLIALDRRTGDRLWESPRTSSLGSVANYVLGVNGRGLFVAGKNVVRRYD
                     LQSGLLKWEQEIENSYGRGCVTDTAVYVPVRDSILQLDLEKGREISQVGVALTSDEPV
                     GNLYSDGDKLWVVGAGRVYAMTTLEHRLAILAEQIAGGDPEAQLNRMRLYFKQNKLEP
                     ALSDLRGAYALFQAQQTPDEAALRLFDAITELKLAQKQPLVALGLLNELFVTAPSPPE
                     LGKEALTRRSDLIATSVSVIRQQKLKSAVAAVLAAAPLITEDYLLSAATFAVDASATK
                     DDAAALIGALQQGDPAAQLLAIRATARVVPDDAKSPLRKLIDKGDDRVRLAAVRALAN
                     LGEQEKVLETLVALMASENPRVRARSHQSLRALTGQKIEFSPEGKAEDQSAAIAAWKQ
                     WISASGKTARLTLPLTEKNVPLGRTLIVSQQQSKVFELGPDNKVRWEKQLPGPAWGCQ
                     GLPNGHRLIAVYSQSLVIEYDEEGNEVWRKDRLPGPPYSVQRLENGSTLVACADAQQL
                     VEIAPDGTQTKTTVEGRPMSAQRLENGNTLVALQQQNRIIELDRSGKIVWEARTQNPP
                     GHATRLESGNTLVCMTYTRQVVEYDSTGKTVVWQSKIPLTNPYAAERLANGNTLIADH
                     QGLKELDAKGEKVIWQHRQPSVTGLSTY"
BASE COUNT          475 a         1004 c          959 g          584 t
ORIGIN      
        1 ggaattgaaa tcatagcggc tagtacgtcg agagccccgt cacgctcggc tgccggtgtt
       61 gccagatgac tttctcgccc ttggcgtcca gctctttcag cccctgatgg tcggcgatga
      121 gcgtgttgcc gttggcgaga cgctcggctg catagggatt cgtcagtgga atcttgctct
      181 gccagacgac cgttttgccg gtggaatcat attcgaccac ttgccgcgtg tacgtcatgc
      241 agacgagcgt gttgccactt tccaggcgcg tggcgtggcc gggcgggttc tgtgtgcggg
      301 cttcccagac gatcttgccg ctgcgatcca gctcaatgat ccggttctgc tgttgaaggg
      361 cgacaagcgt gttgccgttc tccagccgct gggccgacat cggccggccc tcgacggtcg
      421 tcttggtctg cgttccgtca ggagcgatct cgacgagctg ctgggcgtcg gcacaggcga
      481 cgagcgtgct gccgttttcc agacgctgca cgctataagg cggtccgggc aggcggtcct
      541 tccgccacac ttcgttccct tcctcgtcgt actcgatcac caggctctgc gaatacaccg
      601 cgatcagccg gtggccattg ggcaatccct ggcagcccca ggcgggccct ggcaactgct
      661 tttcccaccg cactttgtta tcgggaccca gctcgaaaac cttcgactgc tgctgcgaaa
      721 cgatcagcgt ccgccctagc ggcacgttct tctcggtcag cggcagcgtc agcctggcgg
      781 tcttgccgct cgcggaaatc cactgtttcc aggcggcgat tgccgcgctc tggtcttcgg
      841 ctttgccttc cggcgagaac tcgatcttct ggccggtcag cgcccgcaaa ctttgatggc
      901 tgcgggcccg cacgcgcgga ttctcggatg ccatcagggc gacgagcgtt tccagcacct
      961 tttcctgctc gccgagattc gccagcgctc ggaccgctgc caggcggacg cggtcgtctc
     1021 ccttgtcgat cagctttcgc agcggcgact tggcgtcgtc cggcaccacg cgggccgtcg
     1081 cccgaatcgc caggagttgc gcagccggat cgccctgctg caaggcgcca attagcgcgg
     1141 cagcgtcgtc cttcgtcgcc gaagcatcga cggcgaacgt cgccgccgac agcaagtagt
     1201 cttccgtgat cagaggcgcg gcggcgagca ccgcagcaac ggcgctcttc agcttctgct
     1261 ggcggatgac gctcacgctc gtcgcgatca ggtccgagcg gcgcgtcaac gcctctttgc
     1321 ccagttccgg cggcgaaggc gccgtcacaa acagctcgtt caacagcccg agggccacca
     1381 gcggctgctt ctgggccagc ttcagctccg tgattgcgtc aaacagccgc agggcggctt
     1441 cgtcgggcgt ttgctgggcc tgaaagaggg cgtaagctcc gcggaggtcg cttaaggcgg
     1501 gttccagctt gttctgcttg aaatagagcc gcatccggtt gagctgcgcc tcggggtcgc
     1561 cgccggcgat ctgctcggcg aggatcgcca ggcgatgctc gagcgtcgtc atcgcataga
     1621 cgcgccccgc accgacgacc cacagcttgt cgccgtccga gtagagattg ccgaccggct
     1681 cgtcgctggt gagggcgacg ccgacctgcg agatttcgcg ccccttttcc agatcgagct
     1741 gcaggatcga atcccgcacc ggcacataga cggcggtatc ggtcacgcag ccacggccat
     1801 aggaattctc gatctcctgt tcccatttca ggaggccgct ctgcaggtcg tagcgccgga
     1861 cgacgttctt tccggccacg aacaggccgc gcccgttgac ccccaggacg taattcgcga
     1921 cgcttcccaa ggacgacgtg cggggcgatt cccacagccg atcgcccgtg cggcggtcca
     1981 gggcgatgag gtggtcgcaa tcgctggcga gtatcaccac cgccttgccg tagggaatga
     2041 ccacgtcgtc atcccagcct tccaggtcca gcagacccgt cccctggttg ccgtacatct
     2101 gccgcatcat gttgttgggc ttgccgtcgc gctggtagcg aatcgcccag cggacggtgc
     2161 cgccgaccgc atcgacggcg aacatcaccc cgcagccgca cgagaggtat gcctcgcggc
     2221 catcgacggc gatcgtgatc ggggcccagg ggttggcgcc gccttgcggc tcatcgcaga
     2281 ggtacgactt ccagacggtg tggccatcct cgcggtccag gccgaagagc cagatcgtgc
     2341 cgccatcggt caccggcgcc agcagcagac tgccgcacgg gaccggcgcg gcgaggaaac
     2401 cgacgtcgga attgccttcc ttgtcttcgt cgctggcggt gcggctccag cggactttgc
     2461 cgcccgcggt gctataagcg gtgagccagt tgccgcgcgt tcgccgggga atcacgcccc
     2521 actggaagcc gcgggcgaca gccggctttt gttgtgcttc gcctgtcgcc gagaccttct
     2581 tgccttcgat cgagtagatc acgccgtcgg cgatcgacat cgactggtgg acgcggtcgc
     2641 cgaacaacaa cacttcggcc ggcgaacggg gtttgccgtc ctgcatctgg aagttgtagc
     2701 cgtagttcat ggccatcatg gccatggcct gcgaccggcc gtcgagctcg tattgatttt
     2761 gccagaccga cttccagatc ggacggttgt cgtgggccgt cgtgctgaag gcgacgagtt
     2821 ggtccggcgc cttgataaag atcttgccgc catcgaagag gagctgcccg gcaggacgcc
     2881 agccctgctc gcgccacttc tcgatgaact cctcgcgcga aacgggcgag ggagcggaag
     2941 gctggcgcgc cccccgcccg ctgaaaacga cgatccccat cggcatcgcg ccgccaaagc
     3001 cgtacgggtt attgggattg ac
//
LOCUS       DASUGB010000239         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1833902, whole genome shotgun sequence.
ACCESSION   DASUGB010000239 DASUGB010000000
VERSION     DASUGB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833902"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..997
                     /locus_tag="VFV87_04630"
     CDS             77..997
                     /locus_tag="VFV87_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5103071.1"
                     /translation="MQLGFVSAILPELSLAEVAQLAAETGYDCVELMCWPVSKAERRY
                     AGVTHVDVTDFTSAKADEVRRIMANAGIAISALGYYPNPLSPQAAESELAVAHLRKVI
                     QAAALLGLSTVNTFIGRDWTKSVEDNWPRMVETWRPLVKFAEANKVRIGIEHCPMLFS
                     QDEWPGGKNIATCPKLWRRLFADVGSASLGLNYDPSHMIWQQMDYLRPLREFKDRLFH
                     VHAKDVRLDRERLDDVGILAHPNEYHTPKLPGLGDVNWGQFFSVLTDVGYHGPVCVEV
                     EDRAYEGSLAARKASLVQSCVYLRQYVPRV"
     gene            complement(1148..2266)
                     /gene="ribD"
                     /locus_tag="VFV87_04635"
     CDS             complement(1148..2266)
                     /gene="ribD"
                     /locus_tag="VFV87_04635"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5103072.1"
                     /translation="MAEVSVDLKLMRYALELARRGQGFVEPNPLVGCVIVRDGKIISE
                     GWHQQFGGAHAEIEALLAAGERARGATMYVTLEPCCHYGKTPPCTEAIIPAGLSRVVV
                     AMRDPFPKVAGSGLKQLAAAGIDVELGLGEDEARALNAPYLKLVERGRPWTIAKWAMT
                     LDGKIATRGGYSRWISGEASRQVVHQLRGRVDAIVVGRRTAQLDDPLLTARPEGSAPP
                     RLATRIVLDSQARLASFSQLVRTARQIPTIIVTGPDAEEKELRRLADAGCEVLPFAAA
                     SHYERLLGLLDELGRRRMTNILVEGGGQLLGTLLDARELDEVHVFIAPKFFGGERAPS
                     PLAGAGVEQVAEALSLTGVQVHAVGGYIYVAGRVGRKV"
     gene            2379..>2608
                     /locus_tag="VFV87_04640"
     CDS             2379..>2608
                     /locus_tag="VFV87_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103073.1"
                     /translation="MRLVLGTDEAGYGPNLGPLVVAATAWRVPGEISPAAMYDALAEV
                     VCGGGVPSEEGRLAIGDSKQLYKPGCGLGLLER"
BASE COUNT          447 a          841 c          876 g          444 t
ORIGIN      
        1 gtcggctact ttccgctggg cacggtggcg gcgaagattg cgaaggagta tttgcggcac
       61 gcagcggaga ttgaccatgc aactcggatt cgtcagcgcg attctacctg agctttcgct
      121 ggccgaggtg gctcagcttg ccgcggaaac cggctacgac tgtgtcgagc tgatgtgctg
      181 gccggtcagc aaggccgagc ggcggtacgc cggcgtcacg cacgtcgatg tgacggactt
      241 tacctcggcc aaggccgacg aagttcgccg catcatggcc aatgcgggca tcgcaattag
      301 cgccctgggc tattatccca atccgctttc gccccaggcg gccgaaagcg aattggccgt
      361 ggctcacctg cggaaagtca tccaggcggc ggcgctcttg ggcttatcga ccgtcaacac
      421 gttcatcggt cgcgactgga caaagtcggt cgaggacaac tggccgcgga tggtcgaaac
      481 ctggcggccg ctggtcaaat tcgccgaggc caacaaggtg cggatcggca tcgagcattg
      541 ccccatgctc tttagccagg acgaatggcc gggggggaaa aacatcgcca cctgccccaa
      601 gctctggcgg cggctgtttg ccgacgtcgg ctcggccagc ctgggcctga actacgatcc
      661 ctcgcacatg atctggcagc agatggacta cctccggccg ctgcgcgaat tcaaggaccg
      721 gctgttccac gtccatgcga aggacgtgcg gctggatcgc gagcggctgg acgacgtggg
      781 catcctcgcc catccgaacg aataccacac gccgaagctg ccaggcctgg gcgacgtgaa
      841 ctgggggcaa ttcttttcag tcctgaccga cgtgggctac cacggcccgg tgtgcgtgga
      901 agtcgaggac cgggcgtacg aggggtcgct ggcggcaagg aaggcgtcgc tcgtgcagag
      961 ctgcgtgtat ttgcggcagt acgttccgag agtgtgaaag tgcgatggtg tgagagtatg
     1021 tagggtgcat gcgtccgcag gacgaaatgc accgaccgca gaatcatcac gtcggccgca
     1081 cgctggtgca ttgcgctccg ctcatgcacc ctacttccta ctcccgcacc ctcatcccct
     1141 cgcacgctca caccttcctc cccacccgcc cggcaacata aatgtagccc ccgacggcat
     1201 gcacctggac gccggtcagc gacagggctt cggcgacttg ctcgaccccc gcgccagcga
     1261 gcggcgaggg ggctcgctcg ccgccaaaga atttgggggc gatgaatacg tgtacttcgt
     1321 caagctctct ggcgtcgagc aacgtaccca agagctgacc gcccccttcc accaggatgt
     1381 tcgtcatccg ccgccgcccc agctcgtcga gcagccccag cagccgctcg tagtgcgaag
     1441 cggcggcaaa cggcagcacc tcgcagccgg catcggccag gcggcgaagc tccttttctt
     1501 cggcgtcggg tccagtgacg atgatcgtgg gaatctggcg ggcggtgcgg acaagctggc
     1561 tgaagctggc caggcgggcc tgcgagtcga gcacaatccg cgtggccagg cgggggggcg
     1621 cggacccctc gggccgggcg gtgagcagcg gatcgtcgag ctgcgcggtg cggcggccga
     1681 cgacgatcgc atcgacccgt ccgcgcagtt ggtgaacgac ctgccgcgag gcttcgccgg
     1741 aaatccaccg gctatagccg ccgcgcgtgg cgatcttgcc gtcgagcgtc atcgcccact
     1801 tggcgatcgt ccacgggcgg ccgcgctcga cgagcttgag gtaaggagca ttcagggcgc
     1861 gggcttcgtc ctcgcccagg cccagctcga cgtcgatgcc ggccgcggcc agctgcttaa
     1921 gaccgctgcc ggcaaccttg ggaaacggat cgcgcatggc gacgacgacg cgcgacagtc
     1981 cggcgggtat gatcgcctcg gtgcaagggg gcgttttgcc gtaatggcag cagggctcca
     2041 gcgtgacgta catcgtggcc ccgcgagctc gctcgccggc ggccagcagg gcttcgattt
     2101 cggcatgcgc cccgccgaac tgctggtgcc acccttcgga gatgattttt ccatcccgca
     2161 cgatcacgca gccgacgagc gggttaggct cgacgaagcc ctggccgcgg cgggccagct
     2221 cgagggcata gcgcatcaat ttcagatcga cggaaacttc ggccatgccg tcctctcgtt
     2281 cgccccccct tgttcccgac ccctcattcc cagcggccct ccagtcatta tgattgcggc
     2341 accagggaac ggtagcgcac acggacgaag ggactgccat gcgactggtg ctgggcacgg
     2401 atgaagcggg ttatgggccg aaccttggcc cgctggtggt cgcggcgacg gcttggcgcg
     2461 tgccgggcga gatctcgccg gcagcgatgt atgatgcgct ggcggaagtc gtctgcggcg
     2521 gcggcgtgcc gtcggaggag ggccgcctgg ccatcggcga ttcgaagcag ctttacaagc
     2581 caggttgcgg gcttggcttg ctcgagcg
//
LOCUS       DASUGB010000240         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_444306, whole genome shotgun sequence.
ACCESSION   DASUGB010000240 DASUGB010000000
VERSION     DASUGB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444306"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /locus_tag="VFV87_04645"
     CDS             <1..2100
                     /locus_tag="VFV87_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5103074.1"
                     /translation="RQHASNPVDWYPWGDEALQRARQLDRPIFLSIGYSACHWCHVME
                     HESFEDEEVARLLNEHFVPIKVDREERPDLDQIYMNAVQLMTGRGGWPMSVFLTPDLK
                     PFFGGTYWPPTARMGMPGFEQVLIAVNDAWQNRREAAEKQAEEMTAYLQEDRGQRTED
                     RESGVRSQELSAERMRGLLEGAVRQLARAFDARNGGFGTAPKFPHSMDLQLLLRQWKR
                     RPSDETLRMVTLTLDKMAAGGIYDHLAGGFARYSVDERWLVPHFEKMLYDNALLTSAY
                     LDAFQATKELRFAKIARETCDYILQYMTDPAGGFHSTEDADSEGEEGKFYVWTPPEIK
                     QVLGPDAGERFCYVYDVSEAGNFEHGTSTLNLPKTIEQVAAIKGWNGDELRAELAKSR
                     AKLLAVRDRRVRPSKDDKVLVSWNALMIDAMARTARAIDGNAPALHSSAQALLASAMK
                     AAEFVLKSMSRPDGRLFHTWRHGKAKLDAYLDDYAYLVNALVTLYEATFDERWIDEAA
                     RLADLMLMHFEDKAAGGFFFTADDHEQLIARNKDFHDASVPSGNGMAATALIRLGRLT
                     GRTDFLESARRAIVAGLPTIERSAMAAGQLLIALDMWLGPMHELVLIGGANEAENQAV
                     LNNLRRAFLPNSVIAYRPGQAQGANAPRSPLLDPLFAGRAAMDGQPTLYVCQNFTCRA
                     PVSGVEAIQATLRDLAG"
     gene            2286..2567
                     /locus_tag="VFV87_04650"
     CDS             2286..2567
                     /locus_tag="VFV87_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5103075.1"
                     /translation="MSILEFLLLLLIAGICGSLAQAMVGFSRGGCLVSIALGFVGALL
                     GTWLARAAGLPEIFAVQIGDRSFPIIWSIIGAALFAAVLALISRPRVMV"
     gene            2667..>3662
                     /locus_tag="VFV87_04655"
     CDS             2667..>3662
                     /locus_tag="VFV87_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103076.1"
                     /translation="MPDWSYRTVFRPLLFRLPFGMARDLALGSMGLLSRLPLGSWVIE
                     FLGHMRPDPRLRTPLGPLLLDSPVVIGPWLDRRIVATAALSRFGVGMIELAPGGTAAA
                     NDSRCECDGRECLRIAVDNVEIATNRHFERLERSRPVVPLMLRLAGSAEQVVGRVSNP
                     SDEDRLETCPTAISLATLEQAISEAWPLDDWAKHVESIQFAMPQTPPGNQPLFLVLSA
                     DADFARALPYLRKALEHSIAGLVMDGRIAGGNADWLVGRPALEPALALTRRLREELGS
                     SLPIIAGGADDPQSALELLAAGATAVQIDAALAFAGPGLPKRINDAVLFARCHSPL"
BASE COUNT          637 a         1171 c         1200 g          654 t
ORIGIN      
        1 cgacaacatg ccagcaaccc cgtggactgg tatccctggg gggatgaggc actgcaacgc
       61 gcgcgacaac ttgaccgccc gatcttcctc tcgatcggct actcggcgtg tcactggtgt
      121 cacgtcatgg agcacgagag ctttgaggac gaggaggtcg cgcgcctgct caacgagcac
      181 ttcgtgccga tcaaggtgga ccgcgaggag cggcccgacc tcgaccagat ctacatgaat
      241 gccgtgcagc tcatgaccgg ccgcggcggc tggccgatgt cggtcttcct gacgcccgac
      301 ctcaagccgt tcttcggcgg cacgtattgg ccgccgaccg cccggatggg catgcccggc
      361 ttcgagcagg tgctgattgc ggtgaacgac gcgtggcaga accggcggga ggcggcggag
      421 aagcaggcgg aggagatgac ggcgtacctg caagaggaca gaggacagcg gacagaggac
      481 agggagtcag gggtcaggag tcaggagttg agtgcagagc ggatgcgggg gctgctggag
      541 ggcgcggtga ggcagttggc gcgggcgttt gatgcccgga atggcgggtt tgggacggcg
      601 ccgaagttcc cgcacagcat ggacttgcag ctcttgctcc gccagtggaa gcgccggccg
      661 agcgacgaga cgctgcggat ggtaacgctg acgctcgaca agatggccgc cggcggcatt
      721 tacgatcacc tcgccggcgg cttcgcccgt tacagcgtcg atgaacgctg gctggtgccg
      781 catttcgaga agatgctcta cgacaacgcg ctgcttacca gtgcttatct cgatgcgttt
      841 caagcgacga aggagctgcg tttcgccaag attgcccgcg aaacgtgcga ctacatcctc
      901 cagtacatga ccgaccccgc gggcggcttc cacagcacgg aagacgccga cagcgaaggg
      961 gaggagggca agttctacgt ctggacgccg ccagagatca agcaagtcct cggccctgac
     1021 gccggcgagc ggttctgcta tgtgtacgac gtcagcgaag ccggcaactt cgagcacggc
     1081 acgagcactc tcaatttgcc gaagacgatc gagcaagtcg ccgcgatcaa aggctggaat
     1141 ggcgacgagt tgcgggccga gctcgctaaa tcgcgcgcga agctacttgc agtgcgggat
     1201 cggcgagttc gacccagtaa ggatgacaag gtactggtga gctggaatgc cttgatgatc
     1261 gacgcgatgg cgcgaacggc gcgagcgatc gacggaaatg cacccgcgct ccattcgtcg
     1321 gcccaggcgc tcctggcctc cgccatgaag gcggctgagt tcgtattgaa gtcgatgtcc
     1381 cgccccgacg gccggctttt tcacacctgg cgacacggca aggccaagct cgatgcttat
     1441 ctcgacgatt acgcctacct cgtcaatgcc ctcgtaacgc tctatgaggc gacctttgac
     1501 gaacgctgga tcgacgaagc cgcacggctg gcggacctga tgctcatgca cttcgaagac
     1561 aaagcagccg gcggattctt cttcaccgcc gacgaccacg aacagctcat cgcgcgcaac
     1621 aaggatttcc acgacgccag tgtccccagc ggcaacggga tggcagcgac ggcgctgatt
     1681 cggctgggca ggctgacggg ccgcacggac ttcctggaat cagcccgccg ggcgattgtc
     1741 gccggcctgc cgacgatcga gcgatcggcg atggccgccg ggcaactctt gatcgcgctg
     1801 gatatgtggc tcgggccgat gcatgaactc gtgctgatcg gcggagcgaa tgaggcggag
     1861 aatcaagcgg tattgaataa cctgcgaagg gcgtttttac cgaattcggt catcgcgtat
     1921 cggcccggcc aggctcaggg ggctaacgcc ccccgctcgc cccttctgga cccgctcttc
     1981 gctggccgcg ccgcgatgga cggccagccg acgttgtatg tctgccagaa cttcacctgc
     2041 cgggcgccgg tgagcggggt ggaggccatt caggcgacac tccgagacct cgccggatga
     2101 gccgcgacag tttgtcgcga cgtgtacggc atcgcgcgga tcgaggcgga tcagccctgc
     2161 acatcgctgc atgcaagtca ttatgtgcca actagttgcg attatcggca gcctatctgg
     2221 cacgctggat gcatcgtagc gagcgcattc gcaaagccgc gcctctactt ttggagtcct
     2281 gcccaatgtc cattctcgaa ttcctcttgc tcctactgat cgccggcatc tgcggcagcc
     2341 tggcgcaggc gatggtcggc ttttcgcgcg gcgggtgcct ggtgtcgatc gcgctcgggt
     2401 tcgtcggagc gttgctcggc acgtggctgg cccgcgccgc cggactgcca gagatctttg
     2461 ccgtgcaaat cggcgatcgg agcttcccga tcatctggtc catcattggc gccgccctgt
     2521 tcgccgcggt cctggcgctt atctcccggc cgcgcgtcat ggtgtaaccg gctggcggaa
     2581 agcctgcccg cggtgaatct cgcgcccggt tttcgggtat cttcacggct cggcgagtac
     2641 cgcgctgcca gccgtgacgt gagcccatgc ccgattggtc gtaccgcacc gtcttccggc
     2701 cgctcctgtt ccggctgccg tttgggatgg ctcgcgacct ggccctgggc agcatgggac
     2761 tgctttcgcg gctgccgctg ggaagctggg tgatcgaatt cctcggccac atgcgtcccg
     2821 atccacggct gcggacgcca cttggcccgc tgctgctcga tagccccgtc gtcatcggcc
     2881 cgtggctgga ccggcggatc gtggcgacgg cggccctctc gcggttcggc gtggggatga
     2941 tcgaactggc ccccggcggc acagcagcgg ccaacgacag ccggtgcgag tgcgacggca
     3001 gagaatgtct gcgaatcgcc gttgacaacg ttgagatcgc caccaatcgg cacttcgagc
     3061 gactggaacg ctcgcgccca gtcgtgccgc tgatgctgcg actcgctggg tccgcggaac
     3121 aagtcgtcgg acgggtttcc aacccgtccg acgaggacag attggaaacc tgtcctacgg
     3181 caatttcgct cgcgacgctg gaacaggcca tctccgaagc ctggccgctc gatgactggg
     3241 ccaagcacgt cgaatcgatc caattcgcga tgccgcaaac gccgccgggc aaccagccgc
     3301 tcttcctcgt gttgtctgcc gacgcggact tcgcccgcgc gctgccgtat ttgcggaaag
     3361 ctctcgagca ttccatcgcg ggcctcgtga tggatggacg cattgctggc gggaatgccg
     3421 attggctcgt cgggaggccg gcactcgagc ccgcgctggc gctaactcgc cggctgcgcg
     3481 aagaactcgg cagcagtttg ccgatcatcg ccggtggcgc agacgacccg cagtccgcgc
     3541 tcgaactgct cgctgccggc gcgaccgccg tccagatcga cgccgcactc gccttcgccg
     3601 gaccgggcct tcccaagcgg atcaacgacg ccgtgctctt cgcccgctgc cattctcccc
     3661 tc
//
LOCUS       DASUGB010000241         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_166056, whole genome shotgun sequence.
ACCESSION   DASUGB010000241 DASUGB010000000
VERSION     DASUGB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166056"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFV87_04660"
     CDS             <1..1191
                     /locus_tag="VFV87_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103077.1"
                     /translation="ARLTTRLWAPRFSLRMLLAAFTAVAIGIPVWYRWPYEEEEDIPS
                     RERIVNTWQRQWGGGRLLHGPRTWFRSDGSKQYVTHFVRGERHGRFEAWEPGRPETIG
                     YFEHDQKSGTWTTDTDALRTIQTWKHNQLNGPCEFLWPDGRTERMTFTQGRLTEVNGR
                     PWPSPLYDAHHRRETRDESRAIDDVLYSNLNSPLTINFQDVPLSSAVAHLAADSAGLI
                     PAGGVGTPILLDPRLPDPHLPVTYQVDGLDVKTALVLLLARHELVADYHYGAVWVTTP
                     ELAGPWHDPTGIDKITPPEGSPLAGVWDEPVDVTTTWSTPIYPGQQPLAKVIAEAFGP
                     LMIEIDTSRLAPTTDDPGRFPVVANLQKIPFRHALAYLLFQAKCRCELRGETLVILPP
                     KNEP"
     gene            complement(1188..1430)
                     /locus_tag="VFV87_04665"
     CDS             complement(1188..1430)
                     /locus_tag="VFV87_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103078.1"
                     /translation="MHYLYGSKRDVPRRLVATFGSEQQLRAYVRWATLEEHGPQRGKF
                     EQGSALASYQAWEQSPSPLTDEDATAVVHNPSPSML"
     gene            complement(1524..1862)
                     /locus_tag="VFV87_04670"
     CDS             complement(1524..1862)
                     /locus_tag="VFV87_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103079.1"
                     /translation="MYRPGDRVVYTATKHSSHPGPRAEAVEPERHGEGYSYHVKKYWL
                     VLDVRADGTLVVVTRRGKQRVIPTSDGHLRPARWWETFFLRSRFPQREEIQADNGGSP
                     LPRGDAAVSR"
     gene            1984..2568
                     /locus_tag="VFV87_04675"
     CDS             1984..2568
                     /locus_tag="VFV87_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5103080.1"
                     /translation="MSSTSASLLDRLRDGPQSPAWLRMNDIYQPLIRSWLHRQDVLAS
                     DADDLVQEVLAVVVRRLPDFEHNQRVGAFRTWLKTITINVVRDHWRSKKNRPAATGSS
                     QLHDFLHQLEDQDSGLSRLWDEEHDRHVTHRLLEMLKGDFEPKTWQAFTRMALDGLPA
                     ADVAQELGLTTNAVFIAKSRVLARLRQEAAGMVE"
     gene            2673..>3543
                     /locus_tag="VFV87_04680"
     CDS             2673..>3543
                     /locus_tag="VFV87_04680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103081.1"
                     /translation="MATELYTHPTPDQLRAFAQGRLHGAEMAEVERHVSGCDSCCSHL
                     ERVPDDTLVQLAREAATQGVKSPSAPKTQPPKPSPGEIPEALRDHPRYRVLSVIGHGG
                     MGAVYKAEHRKMERLVALKVINQVHLAHPQAIERFGREVRAVSRLSHPNIVLVHDADE
                     AGDLHFLVMEFVDGISLDRVVAHNGPLSITHAVQFIRQAALGLQHAHEKGMVHRDIKP
                     QNLMLTRTKHEIKILDFGLARLAGEQAALGAGEGSDPGTKPSQTTAGMILGTPDYIAP
                     EQASDARTADIRAD"
BASE COUNT          636 a         1143 c         1154 g          610 t
ORIGIN      
        1 gcacgattga caactcgcct ctgggcgccg cgattcagct tgcgcatgct cctggcggcg
       61 tttaccgcgg tagcgattgg gattccggtt tggtatcggt ggccgtacga agaggaggag
      121 gatatcccat cgcgtgaacg gatcgtcaac acttggcaac ggcagtgggg tggcgggcgc
      181 ttgctgcatg ggcctcggac gtggtttcgg agcgatggat ccaagcagta cgtcacgcac
      241 tttgtccgcg gcgagcgtca tggccgcttt gaagcctggg agccgggacg gccggagacg
      301 attggctact tcgagcacga ccagaagtcg ggaacgtgga cgaccgacac cgatgcgttg
      361 aggactattc aaacctggaa gcacaatcaa ctgaacggtc cgtgcgaatt cctctggccg
      421 gacggtcgga cggagcggat gacgttcacg cagggccggc tgacggaggt gaatggccgg
      481 ccgtggccgt cgccgctgta tgacgcgcac catcggcgcg agacgcggga tgaatcgcgg
      541 gcgatcgatg atgtgctgta ttccaacctc aacagcccgc tcaccatcaa ctttcaagac
      601 gtgcctctct cgtcggcggt ggcgcacctg gcggcggatt ccgcgggact catccctgcc
      661 ggcggagtcg gcacgcccat cctgctcgat ccgcggttgc ctgacccaca cctcccggtc
      721 acgtatcaag tggacgggct ggatgtgaag acggcccttg ttctgctgtt ggccaggcac
      781 gagctcgtgg cggattatca ctatggagcc gtttgggtca cgacgccgga attggccggt
      841 ccgtggcacg atccgaccgg catcgacaag atcactccgc cggaaggctc accgctggcc
      901 ggcgtttggg acgagcccgt ggacgtgaca acgacctgga gcacgccgat ctatcccggc
      961 cagcagccat tggccaaagt aattgccgag gcgttcggtc cgttgatgat cgagatcgac
     1021 acgtcgcgcc tcgcgccgac gaccgacgat cccggtcgct ttcccgtggt ggccaatctc
     1081 cagaagattc cgttccgcca tgccttggca tacttgctct tccaagccaa atgccgctgc
     1141 gagctgcgcg gcgaaacgct agttatcctg ccgcctaaga atgagcctta gagcatgctc
     1201 ggcgacggat tatggacgac ggcggttgcg tcttcgtccg tcagcggcga aggcgattgt
     1261 tcccaggcct gatagctggc caaggcgctg ccttgctcga acttgccgcg ctgggggccg
     1321 tgctcttcca gcgttgccca gcggacgtag gcccgcagct gctgctcgga accgaacgtg
     1381 gcgaccaatc gccgcggcac atcgcgcttc gagccgtaga ggtaatgcat ggccgtgccg
     1441 cctaagtgga ctcgcagacg aaaccgtagg acgggttttc aacccgtctg ccgtaggaca
     1501 ggttataaac ctgtcctacg aaactagcgc gacacggccg catcgccacg gggcaggggg
     1561 gagccgccgt tgtcggcctg aatctcctcg cgctgcggaa agcggctgcg gaggaagaag
     1621 gtttcccacc agcgggccgg gcggagatgg ccgtcgctcg tggggatgac ccgctgcttg
     1681 ccgcggcggg tgacgacaac cagcgtgccg tccgcccgca cgtcgagcac cagccagtac
     1741 tttttgacgt ggtaagaata gccctcgcca tgtcgctcgg gctcgaccgc ctcggcccgc
     1801 gggccgggat ggctgctgtg ctttgtcgcg gtgtagacca cgcggtcgcc gggtcggtac
     1861 atgcctccag ccctgtcgcc tgaagtagcc gtcagtccat aatactatgc atcgtgccca
     1921 atggcttcaa tacttaaacg ctgattctca gtcgttgcgg cggtcgttct ttcggatttc
     1981 agcatgtcct ccacctccgc cagccttctc gaccgcctgc gcgacgggcc gcaatcgccg
     2041 gcttggctgc ggatgaacga tatctatcag ccgttgatcc gctcgtggct gcaccggcag
     2101 gacgtgctgg ccagcgacgc cgacgacctg gtccaggagg tgctggccgt ggtcgtccgc
     2161 cgcctccccg atttcgagca caaccagcgg gtcggggcgt tccgcacctg gctcaagacg
     2221 atcacgatca acgtcgtccg cgaccactgg cggagcaaga agaaccgccc cgccgccacc
     2281 ggcagcagcc agcttcacga tttcctccac cagctcgaag accaggacag cggcctttcg
     2341 cggctgtggg atgaagagca cgaccgccac gtgacgcacc gcctgctgga gatgctcaaa
     2401 ggcgacttcg agcccaagac ctggcaggcg ttcacgcgga tggccctgga cggccttcct
     2461 gcggcggacg ttgcccagga gctcggcctg acgacgaacg cggtgttcat cgctaagtcc
     2521 cgcgtgctgg ccaggttacg gcaagaggcg gcggggatgg tggaatgagc ttagccgccg
     2581 cgaccacgcg gcgctttggc tgccttgtga aaggagcccg cccgctgtgc catactgata
     2641 ttgcggattg cggattggag gcagggcgaa ttatggccac cgaactttac acccatccga
     2701 cgcccgatca gctccgggcc tttgcccagg gtcggctgca cggggcggaa atggcggaag
     2761 tggagcggca cgtgagcggg tgcgattcgt gctgctcgca cctggagcgc gtgccggacg
     2821 acacgctggt gcagctcgcc cgcgaagcgg cgacgcaggg agtcaagtcg cccagcgccc
     2881 caaaaacgca accgcccaag ccttcgcccg gcgaaattcc cgaagcgctc cgcgaccacc
     2941 cgcgctaccg cgtcctcagc gtcatcggcc acggcggcat gggggccgtc tataaggcgg
     3001 agcaccgcaa aatggagcgg ctggtggccc tgaaggtcat caaccaggtg cacctggctc
     3061 atccgcaggc gatcgagcgg ttcggccgcg aagtgcgggc cgtctcgagg ctctcgcacc
     3121 cgaacatcgt gctggtccac gatgccgacg aggccggcga cctgcacttc ctggtgatgg
     3181 agttcgtcga tggcatcagt ctcgaccgcg tcgtcgccca caacggcccg ctgtcgatta
     3241 cgcacgccgt ccagttcatc cgccaggccg cgctgggcct gcagcacgcc cacgagaagg
     3301 ggatggtcca tcgcgacatc aagccgcaga acctgatgct cacgcggacg aagcacgaga
     3361 tcaagattct ggacttcggc ctggcccggc tggccggtga acaagccgcc cttggggcag
     3421 gggaggggag cgacccaggg accaagccga gccagacgac agccggcatg atcctcggca
     3481 cgcccgatta catcgccccg gagcaggcca gcgacgcccg caccgccgat attcgggcag
     3541 ata
//
LOCUS       DASUGB010000242         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_722239, whole genome shotgun sequence.
ACCESSION   DASUGB010000242 DASUGB010000000
VERSION     DASUGB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722239"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFV87_04685"
     CDS             <1..595
                     /locus_tag="VFV87_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5103082.1"
                     /translation="SLDFARLLVASGADVNLRLKKGISGRGILSRVGATPFLLAASTA
                     DVPLIRTLVELGADPLLANSEHATPLMAAAGLGCRAPGEEAGTEAEAMEAVKLAIELG
                     GDVNAVDDNGETAMHGAAYKSLPQVVALLTERGAKIDVWTKKNKWGWTPLSIAEGYRV
                     GNFKPSAETILALHRVLQSAGIEPPASSAPDGKRKSY"
     gene            complement(728..1726)
                     /locus_tag="VFV87_04690"
     CDS             complement(728..1726)
                     /locus_tag="VFV87_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5103083.1"
                     /translation="MRLPQFLQIEPVGQCNLRCQMCPVQFRRDGPAGGGMAGMDYSVF
                     TRLIDQFITVRELHLQGLGEPMMHPRLFDMIEYAAVKGIRVTITTNMTLLSPRRAERC
                     VTSGLAGMCISVDSADPATYERIRVGSCFERVERNVRLLNETRRRHGGENPPLRLVGV
                     LMRQTLEGLPDLVRLADRWSIRELFVQHLCHDFAESSLPDEYRSMRDFVDRQTLVTED
                     RAVIEDAFDRARRTADELGVQLRLPHPRPRPHPPGTPGPKRCDWPWARSYVSYQGLAM
                     PCCIVATPDRANFGSMKDEGVEAIWNGPSYRRFRDQLASDEPPEVCKSCAVYAQTF"
     gene            complement(1874..>2745)
                     /locus_tag="VFV87_04695"
     CDS             complement(1874..>2745)
                     /locus_tag="VFV87_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5103084.1"
                     /translation="YGGIAATLAAVDRIVEVLDEAEPPPAPPRRRPRRIRGQLQLRNV
                     SFAYAGGPLVLDRVNLRVESGATVGILGASGAGKSTLLSLIPRLYDVPPGSRISLDRC
                     DIRAFAAAALRRNVLLVPQQARLFEGTLRQNLTYAAPRADERLIWRTLEAVDLAGLVQ
                     SLPDGLDTWVGERGASLSGGQRQRVALARALIVRPPVLLLDDCTSALDPQTDALVRRR
                     IAELRPDQTRLVVSHRPQSLEGADWIVELRGGRIVRQGTPEEMLSDHFLHVPSLCTLP
                     VIRPSVHSRTASL"
BASE COUNT          476 a          873 c          958 g          438 t
ORIGIN      
        1 cagtctggat ttcgcgcggc ttctcgtggc cagcggggcg gacgtgaacc tgcggctgaa
       61 gaaggggatt tcgggccgcg gaattctcag ccgcgtgggg gcgacgccct ttttgctggc
      121 cgcctcgacg gccgacgtgc cgctgattcg gacgctggtc gagctagggg ccgatccgct
      181 gctggcaaac agcgagcatg ccacgccgct catggccgcg gccgggcttg gctgccgggc
      241 tcccggcgaa gaggccggga ctgaggccga ggcgatggaa gcggtgaagc tggcaatcga
      301 gctcgggggc gatgtgaacg cggtggatga caacggcgag acggccatgc acggggccgc
      361 gtacaagagc ctgccgcagg tcgtcgctct gctgactgag cggggggcga agatcgacgt
      421 gtggaccaag aagaacaagt ggggctggac gcccctctcg atcgccgagg gctaccgcgt
      481 cggcaacttc aaaccgtcgg ccgaaacgat tctcgccctt caccgcgtgc tgcagtccgc
      541 cggcatcgaa ccgcccgcca gttcggcacc agacgggaaa aggaagagct attgaagcct
      601 gacgcgcaag cgaaggaatg ttatcacttt tttgtccttc gcttgcgctt caggcatcag
      661 cgccgttgtg ctcgtccttc gcttgcgatt caggcttcag cgaggcaatg gattctctga
      721 agttttgtca aaacgtttgg gcgtacaccg cgcaggattt gcacacttcc ggcggctcgt
      781 cggaagcgag ctggtcgcgg aatcgacggt agcttggtcc attccagatt gcctcgactc
      841 cctcgtcctt catgctgccg aagttggctc ggtcgggcgt cgcgacgatg cagcacggca
      901 tcgccagccc ctgatagctg acgtacgacc gggcccaggg ccaatcgcac cgcttgggtc
      961 ccggagtgcc gggcggatgc ggacgcgggc ggggatgcgg caggcggagt tgcacaccca
     1021 gctcgtcggc agtgcggcga gcccggtcga aggcgtcctc gatgaccgcg cgatcttcgg
     1081 tcacaagcgt ctggcgatct acgaagtcgc gcatcgagcg gtactcgtcc ggcaggctgg
     1141 attcggcgaa gtcgtggcat aggtgctgga cgaatagctc gcggattgac cagcggtcgg
     1201 ccagccgcac caggtcgggc aggccctcga gcgtttgccg catgagcacg cccaccagcc
     1261 gcagcggagg attctcgccg ccgtggcggc ggcgggtttc gttgagcagg cgtacgttcc
     1321 gctcaacgcg ctcgaagcaa gagccaacgc gaatccgctc ataagtcgcc gggtcggccg
     1381 aatcgaccga gatgcacatg ccggccaggc cgctggtgac acaccgctcg gcacgccgcg
     1441 ggctgagcag cgtcatgttg gtcgtgatcg taacgcgaat ccccttaacc gccgcgtact
     1501 cgatcatgtc gaacagccgc gggtgcatca ttggctcgcc caggccttgc aggtgcagct
     1561 cgcggacggt gatgaactgg tcgatcagcc gggtgaagac ggaataatcc atgccggcca
     1621 tgcccccccc cgcgggtccg tcgcggcgaa attggacggg gcacatttgg cagcgcaggt
     1681 tgcactggcc caccggctcg atctgcagga actggggaag tcgcaaggcc gagagatccg
     1741 ctgcgagcag ggaaacatgc gccggccgcc cacgcggctc aatactgctt gtcgtgcaag
     1801 tcccgttcca gcgtttgcgc cgcgaagcga gcgaacacag ggccggctcg gcgcggtggg
     1861 ccggcgcgtc gcgtcagagc gacgcagtgc gggagtgcac gctggggcga atcaccggca
     1921 gcgtacaaag ggacgggaca tgcagaaaat ggtcgctcag catttcctcg ggcgttccct
     1981 ggcggacgat gcggccgccg cgcagctcga cgatccaatc ggctccttcc agcgattggg
     2041 gccggtgcga aaccaccagc cgcgtctggt cggggcggag ttcggcgatc ctgcggcgaa
     2101 cgagggcgtc ggtttgtgga tcgagggcgc ttgtgcagtc gtcgagcaac agcacgggcg
     2161 gccgcacgat gagcgcccgg gccagcgcga cccgctgtcg ctggcctccc gagaggcttg
     2221 cgccgcgctc gccgacccag gtgtccagcc catcgggaag cgactggacc agcccggcca
     2281 gatcgaccgc ttcgagcgtt cgccagatca gccgctcgtc ggccctcggg gcggcatatg
     2341 tcagattctg ccgcagcgtt ccctcgaaga gccgcgcttg ctggggcaca agcagcacat
     2401 ttcgccgcag cgccgccgca gcgaaggccc gaatgtcgca gcggtcgagc gagatacggc
     2461 tgccgggcgg cacgtcatac agtctgggaa tcagcgacag aagcgtgctt ttaccggcgc
     2521 cgctagctcc caggatgccg acggtcgcac cggactcgac gcgcaaattg acgcggtcga
     2581 gcaccagcgg cccgcccgcg taggcaaacg aaacattgcg cagctgcagc tggccgcgaa
     2641 ttcttcgcgg ccggcggcgg ggaggcgcgg gagggggctc cgcctcgtcg aggacctcaa
     2701 cgatgcgatc gaccgccgcc agcgtggccg cgatgcctcc ataga
//
LOCUS       DASUGB010000243         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1000836, whole genome shotgun sequence.
ACCESSION   DASUGB010000243 DASUGB010000000
VERSION     DASUGB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000836"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1136
                     /locus_tag="VFV87_04700"
                     /pseudo
     CDS             63..1136
                     /locus_tag="VFV87_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1139..1255)
                     /locus_tag="VFV87_04705"
                     /pseudo
     CDS             complement(1139..1255)
                     /locus_tag="VFV87_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
     gene            1637..2215
                     /locus_tag="VFV87_04710"
     CDS             1637..2215
                     /locus_tag="VFV87_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5103085.1"
                     /translation="MILGSILIEMTDVTQIFSQIEQGDRSAAERLLPLVYDELRKLAA
                     AKLAQEKPGQTLQATALVHEAYVRLVDGETEQQFNSRGHFFAAAAEAMRRILVEQFRR
                     KNQVKHGGEYLRVELDAIEPETSGQQDGILALHESLNRLEAHDSLAASVVKLRYFAGL
                     SMPEAAEALGIPLRTAERNWTYARTWLLRDLE"
     gene            2665..>3631
                     /locus_tag="VFV87_04715"
     CDS             2665..>3631
                     /locus_tag="VFV87_04715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103086.1"
                     /translation="MLKARDEKLQRIVAVKTLAEPLAASGTARQRFVREAIAAAAIRH
                     ENVVDIHAVDDGGPVPYLVMEFIDGRSLEARLRDAGSLELKELLRIGLQIAEGLAAAH
                     KQGLIHRDVKPANILLENGVPRVKITDFGLARTADDASLTATGLIAGTPAFMSPEQAK
                     GEAIDQRSDLFSLGSVLYAMCTGQSPFRGSTTLGVIKRVCEDEPTPIRELNADIPEWL
                     CGIVKRLHAKRPDERYQTAAEVAEDLRRGLALVQGGVPQPPIQEPASAIRAHGALGAP
                     RRTVRRWLVPAVATALMLALTLTLTEAGGVTHLVPTVIRVVTGEGT"
BASE COUNT          739 a         1146 c         1092 g          654 t
ORIGIN      
        1 tgctgcttta ttgccagcct cagccgcacc gtgtgtgctg tccccaatgt gggagccgcg
       61 acgtgatctt gcgcggcgag aaagaccgct ggattcgcaa cctgccgatc ggacgggatt
      121 gcacctggat catcgccacg ctcccccgcg tcgagtgccg agcctgccgc acggtccgcc
      181 aagtccacat cgggctggcc gacacccgcc gcacgtacag ccacgccttc gaacgatatg
      241 tcctggaact ctgccggcgg atgaccatgc aggacgtggc ggagcacctg ggagtgagct
      301 gggacatcgt caaggacatc cagaagcggc acctgcagaa gcactacgcc cagcccgcct
      361 tgaaggacgt tcgccaaatc gccatcgacg aaatctgcgt gggccgcggc taccgcttcc
      421 tgactctggt cctggacctg gagagcgggg ccatcctgtt cgtcggacag gggaaaaagg
      481 ccgacagttt gcggccgttc tggcggcggc tgcgggcggc gcgggcgaag atcaaggccg
      541 tcgccatcga tatgtccccg gcctatcagc aggccgtgac caaacacctg ccaggcgcca
      601 tcgtggtctt cgaccggttc catgtcgtca agctgctgaa caagaagctg actcaactcc
      661 gccgccagct ctatcgccag gccaccagcg acctgaagaa aaaggcgctc aaaggcaccc
      721 gctggctgct cctcaagaat cccgagaacc tcgatcccgt gaagggagag ccggggcgac
      781 tgcgggccgc cctccgcctc aacgagtcgc tggccaccgc ctattacctc aaggaagacc
      841 tgcggcaaat ctgggagcaa ctcggcaagt tcccggccag gatcaagctc ctcgattggt
      901 atcatcaggc gatggactcc ggcatccgcg tcctttagga cttcgcccgc accctcctgg
      961 cccacgccga cggcatcctc gtctggtacg actatcccat ctccaccggc cccctcgaag
     1021 gcaccaacaa caaactcaaa accctcaacc gccagcacta cggactccgc gaccccgact
     1081 tcttctccct caaactctat caactccacg aaaccaagta cgctttagtc ggatgaaccg
     1141 ctagattcac tgccgccagc ttcctcaact cctcataaac tagcggcaga agctgctcaa
     1201 cggcaccggg atcaccttgg tcaatctgcg agagaatttg cgtaacgtcg gtcatttcga
     1261 ccaggatact gccgagaagt tggtagaagc caatagctgt gctcggttga tccgacccta
     1321 tggcaaatcc ccagagctga tgctgccaag ttgtgcagag gtcaaacaga cctcccgcag
     1381 taagcggtcg ctcttcctgc aggcggttgg accaacaagc gcaagtttcg gccggcgaat
     1441 acttgcagcg gtgtcctgcg ggaagagaca ccgctgcagt tggcaagatc ttcgttgcgg
     1501 gcgtggccga tgcaaccgct ggcagttcaa gctgcgcgaa actgcgggcg gtgtgtctca
     1561 cctccgcgcg ctatctggcg gcctcagctc tgggtcttgc cgagacgtcg gccctcatcg
     1621 caactgatgg cagccgttga ttctcggcag tatcctaatc gaaatgaccg acgttaccca
     1681 gatattctcg cagattgagc agggggatcg gagcgccgct gagcgactgt tgccgctggt
     1741 gtatgacgag ctgcgcaaat tggccgcggc aaagctcgcg caggagaagc ctgggcaaac
     1801 gctgcaggcg acggcgctag tccatgaagc ctacgtccgg cttgtcgatg gcgagacgga
     1861 acaacaattc aatagccgcg gacacttctt tgccgcggcg gcagaggcaa tgcggcggat
     1921 attggtggaa cagtttcgcc gcaagaatca agtcaagcac ggcggcgagt atctgcgcgt
     1981 cgagctcgac gcgattgaac cagaaacatc tggacagcaa gacggaatac tggcgcttca
     2041 cgaatcgctt aatcgtttgg aagcacacga ctccttggcg gccagtgtag tgaaacttcg
     2101 ctacttcgcc ggtctgagca tgccggaagc agctgaggcc cttggaattc cgctccgaac
     2161 tgctgagagg aactggactt acgcgcggac gtggttgctg cgagatctcg agtagtgtcg
     2221 cgaaatggct gaacaatcag ctgctacaga cacaatcgcg gcaaattttg gcggggttgg
     2281 cctgctttct tcgcactgtt tcgtagggcc aaaggttggc cgggagaatt ctccgtgaat
     2341 tcgcgacgaa cgagcgccaa gacaatattt ctccaagcct tggagctgac aaccgccgcc
     2401 gagcgccaag cctgcctcga cacgcagtgt ggcagtgacc cccaactgcg ccgcgaagtg
     2461 gaggagttgc tgcgccatca agagaatgtg ggaaacttcc tcgagtcacc accaactgcg
     2521 gttgtagact ccgccgagcc ccttcgggcg ctggcgcccc taccgtcgct ggcatttctg
     2581 ataccgtctt ctcaacctga ctcgcttgga cgtctcgggc actatgacat cctggaagtc
     2641 gttggcagcg gcgggtttgg ggtcgtgctc aaagcccgcg atgagaagct ccagcgaatt
     2701 gttgccgtca agacgctggc cgaaccgctg gccgcatccg ggaccgcccg ccagcggttt
     2761 gtgcgtgagg ccatagccgc tgcggccatt cgtcacgaaa acgtcgtgga catccacgcc
     2821 gtggacgatg gcggaccggt cccgtattta gtgatggagt ttatcgacgg ccggtcgctg
     2881 gaagcgaggt tgagggacgc cggttcgctg gagctgaagg aactgctccg tatcggactg
     2941 caaattgccg agggactggc cgctgcccac aagcagggac taatacaccg cgacgtgaag
     3001 cctgccaaca ttctgctgga gaacggcgtc ccgcgggtca agatcaccga tttcggcctg
     3061 gctcgcacgg ccgacgacgc ctcgctgacg gcaaccggac tgattgccgg aacgcccgca
     3121 ttcatgagtc ccgagcaggc caagggtgaa gcgatcgatc aacgttctga cctcttcagt
     3181 ctgggcagcg tcctctacgc catgtgcact ggccagtcgc cgtttcgcgg cagcaccacc
     3241 ctgggcgtga tcaagcgggt ctgtgaggac gaaccgacgc cgatccgcga gttgaatgcg
     3301 gacatccccg agtggctgtg cggaattgtc aagcggctgc acgccaaacg tccggacgag
     3361 cgctaccaga cggcagcgga ggtggcagag gatttgcggc gcgggctggc gctggtgcaa
     3421 ggcggagttc cccagccccc gattcaagaa cctgcatcag cgatccgcgc gcatggcgca
     3481 ctaggtgcgc cccggcgaac cgtgcgccgc tggctcgtgc cggccgtcgc cacagcgctg
     3541 atgctggcgc tcacgctcac cctgaccgaa gccggcggcg tgacgcacct ggtgccgacc
     3601 gtcatccgcg tcgtcaccgg cgaaggaacg c
//
LOCUS       DASUGB010000244         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_583202, whole genome shotgun sequence.
ACCESSION   DASUGB010000244 DASUGB010000000
VERSION     DASUGB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583202"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFV87_04720"
     CDS             <1..276
                     /locus_tag="VFV87_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX5103087.1"
                     /translation="DLDPAAAFAKVRPALEKTAQDFQGQDLQIEAGDTSATFSFKSLG
                     FSITGQAQALAGEVLVNVDLPFAAMMFKDRAEKGIEKNVARALLNAN"
     gene            complement(678..2711)
                     /locus_tag="VFV87_04725"
     CDS             complement(678..2711)
                     /locus_tag="VFV87_04725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103088.1"
                     /translation="MAPARVIEAGIAQHGPGPCPAEGDLQAFAEGRLTEAASDSIDRH
                     VTQCGRCAETLVRLSDRVPFPLNAKAIAGGTSSNAEAVIREPECMRMQKAAREIPLRF
                     GDQDSHDASQESTDSTRLTTDNPAAKRLDRSPPQGEATQAFLPSTPRAIGRYQVKTLL
                     GEGSYGRVYLAHDPQLDRQVAIKVAKFGPNVRRALIDEFLREAKIAAKLKHPGIVTVY
                     DSGEDEQAGCYIVMEYVEGRSLKRLMADEKVPHDKAADYIAQAAEALAYAHKRDLVHR
                     DIKPANLLVDHDGRIKIADFGLAIFEEEQRKRGNEFAGTLAYCSPEQIRGDVPHLDGR
                     TDIWSLGVILYELLTGRRPFGGSKIADEILHRPAKPPRQIDDTIPPQLETSCLRCLHQ
                     QAGDRHSTALDLAEDLNQWRFGSNDSASSRLNKSQSRISGAQPSSESTPRSRTDDGVV
                     DPPGTLRRWMLVASATVLAIASLSVGIVAWQYSRAGNSNIESPEVVAWFPHDRYDAHG
                     FDHQNKCYRFDAFGHALFATDSLTGECSHITACFSTKKWLGFSGVFWNLHMDADGSYR
                     CFCARLGRYDDGRQFKLEVCEFEIGPLPGGWQGVRSEDVLMSEFLPLPQGDRVSLKVR
                     IAESDVARVWVNGEAVLEKPCQLLARNDPTPPSSKVGFCGQLGEITFFSFSAR"
     gene            complement(2730..3350)
                     /locus_tag="VFV87_04730"
     CDS             complement(2730..3350)
                     /locus_tag="VFV87_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5103089.1"
                     /translation="MTDTPVFIDKESGTSTGLLRRAVLRQPDAWDKLVSLYGPLVYRW
                     CRRWGLQPRDAENVGQEVFVRVLLGLPKLQIDGANRSFRGWLFRIARNCFLDHIRDQR
                     TVVQGAGGSTGQEWLEQVPAAAVSDESESATSHEDNNILIQQALGLIRGEFSDRDWNA
                     FSQVVMQGRLPADVAADMNISTNVVYLVKSRVLRRLREEFAELVEF"
     gene            3644..>3904
                     /locus_tag="VFV87_04735"
     CDS             3644..>3904
                     /locus_tag="VFV87_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103090.1"
                     /translation="MLRILSSLLFLALTAGIPCRGLGDDAEPLHARIDRLIDAQTGGP
                     LADDSSDVEFLRRIYLDVVGRVPSAEETRAFLADSNADKRAAL"
BASE COUNT          777 a         1185 c         1105 g          837 t
ORIGIN      
        1 gacctggacc cagccgctgc cttcgccaag gtgcgtccgg cgctggaaaa gaccgcccag
       61 gattttcaag gccaggacct gcagatcgaa gccggcgaca cctccgccac cttcagcttc
      121 aaaagcctcg gcttttccat cacaggccag gcgcaagccc tggccggcga agtcctcgtc
      181 aacgtcgatc tccccttcgc cgcgatgatg ttcaaagacc gggcggagaa ggggattgag
      241 aagaatgtcg cgcgggcgct gctgaacgct aactaagccc ccctggaccg ggtgcgaggt
      301 ttgcgcagtc cggacttgtc acggacttga aggcagttga tcccgaattg agcgggggtt
      361 cagtctcaaa tctacatttg atttcaacgc tttgtggctc gcttggatgg ttcgattgac
      421 tggtttcata atcgtgcgac gagccggtaa acgcgaggtc aaagttctgg gttcctccac
      481 ccacaaacgt aaccctcgca tagggattct gtgcatcttc cgttccggct gtaaaccttt
      541 ggactcgatg gccagcagtt ggaataagct gtgctcgaac acaaaaattc caaccgaatc
      601 cgttggtatc gtgcttctga gctcggacgt ttgtgataat tccgctcact tgtcgatctc
      661 ctgggtgtcg tggaattcta cctggccgaa aatgaaaaaa acgtgatttc cccaagctgc
      721 ccacagaatc cgactttcga cgacggaggg gtgggatcgt tgcgagcgag aagttgacat
      781 ggtttctcga gaacggcctc tccgttgacc caaacccgcg ccacatccga ttcagcaatc
      841 cgaaccttca gacttactcg atcaccctgc ggcagcggta agaactctga cataagaaca
      901 tcttctgacc tgaccccttg ccaaccgcct gggagtggtc cgatttcgaa ctcgcagact
      961 tccaacttga actgtcgccc atcatcgtac cgccccagtc tggcgcaaaa gcatcggtat
     1021 gaaccatccg cgtccatgtg tagattccaa aatactccgc taaatcccag ccatttctta
     1081 gtcgagaagc acgcagtaat gtggctgcac tcgcccgtca gcgagtcggt agcgaagagt
     1141 gcgtgcccaa aggcgtcgaa gcggtagcac ttgttctgat gatcaaatcc atgggcgtcg
     1201 taccgatcgt gcggaaacca ggcaacaact tcgggagatt cgatattcga attgcccgct
     1261 cgagagtatt gccacgcgac gattcccacc gataggctcg cgatcgccaa taccgttgca
     1321 gaagcgacaa gcatccacct tcgtaacgta ccgggcgggt caacgacgcc atcgtctgtt
     1381 cgcgatcgcg gcgtggattc tgacgagggt tgcgcgccgg aaattcgcga ctgggactta
     1441 ttcaggcgac tgctcgctga atcatttgat ccgaaccgcc actgattcag atcttcggcc
     1501 aggtccagtg ccgtcgaatg tcggtcaccc gcttgctgat gcaagcatct gaggcaacta
     1561 gtctccaact gtggcggaat cgtatcatca atctggcgag gtggcttcgc cggtcgatgc
     1621 aggatttcgt cggcgatttt cgaaccgcca aagggccggc ggccggtgag gagttcgtag
     1681 agaatgacgc ccagactcca aatatcggtg cgcccgtcga ggtgcggaac gtcgccccgg
     1741 atttgttcgg gtgagcagta ggcgagcgtc cctgcgaatt cgttgccgcg tttgcgttgc
     1801 tcttcctcaa aaatcgccag gccgaaatcg gcaatcttga tcctgccgtc gtgatcgacc
     1861 agcagattgg ccggcttgat gtcccggtgg acgaggtcac gcttgtgggc ataggccagc
     1921 gcttcggcag cctgagcgat gtaatcggca gctttgtcgt gcgggacctt ttcgtcggcc
     1981 atcaggcgtt tgagcgacct gccttcgacg tattccatca cgatgtaaca gccggcctgc
     2041 tcgtcttctc ccgagtcata aaccgtcaca attcccgggt gtttcagctt cgcggcgatt
     2101 ttggcctcgc gcaggaattc gtcgatcaag gcccggcgaa cgttcgggcc gaacttggcg
     2161 accttgatcg ccacctggcg gtcgagctgc ggatcgtggg ccagatagac tcgcccatac
     2221 gatccctcgc cgagtaatgt cttcacctgg tagcggccaa tcgctcgggg agtgctcggc
     2281 aggaacgctt gtgtcgcctc gccctgtggc ggcgaccggt cgagcctttt ggccgccgga
     2341 ttatcggtcg tgagccgcgt ggaatccgtc gattcctggc tggcatcgtg cgagtcctga
     2401 tcgccaaacc gcaagggaat ctcgcgggca gccttctgca tccgcatgca ttcgggctcg
     2461 cgaatcacgg cctcggcatt tgaacttgtt ccgccagcaa tcgccttggc attgagcggg
     2521 aacggaacgc gatcggagag gcgaacgagc gtctcggcac agcgcccgca ctgcgtgacg
     2581 tggcgatcga ttgaatccga cgccgcttcc gtcagcctgc cttcggcaaa agcctgcaga
     2641 tcgccctcgg ccggacacgg gcctggaccg tgctgggcaa ttccggcttc gataactcgt
     2701 gcaggagcca tctgcggcgc ctcaaaaggt cagaactcta ccaactccgc gaattcctcc
     2761 cgcagccgcc gcaggacgcg agattttacc agatagacga catttgtgct gatattcatg
     2821 tcggctgcca catcggccgg caagcggccc tgcatcacga cttgcgaaaa cgcattccaa
     2881 tcccgatcgg aaaactcccc gcgaatcaac cccagcgcct gctggatcaa gatgttgttg
     2941 tcctcgtggg acgtcgctga ttcactctcg tcggaaacgg ccgctgccgg gacctgctcc
     3001 agccactcct ggcccgtgct gccgccggcc ccttgcacca ctgtccgctg gtcgcggatg
     3061 tgatccaaga aacagttacg agcaatgcga aacaaccagc cacggaatga tcgattggca
     3121 ccgtctatct ggagttttgg cagtcccagc aaaacccgga cgaacacctc ctgccccacg
     3181 ttctctgcat ctctcggttg caagccccag cggcggcacc agcggtaaac cagggggcca
     3241 tacaggctca ctagtttgtc ccacgcgtcc ggctgccgga gcacagccct gcggaggagc
     3301 cctgtggacg taccgctctc cttatcaata aaaacgggag tgtctgtcac gattttgtac
     3361 cggaactacg aggcacaccc gacccgcctc cgtaagccgg tggtgctccg aacattgtgt
     3421 cgcactggga aacgattttc caccggcttt tttacggaga tttgtgggtt tggttcagcg
     3481 gctgagaggt cgagcggtga acggatcgcg gcgccaacac gctgatcgac cttccgcacg
     3541 gcgatttttc gcgggattcg ctttaacctt ggccggctag cccttacact aaagatcaat
     3601 tcccgccgat acgatcccgc ctcccgaccg cctgaggttt ctgatgctga ggatactcag
     3661 ctcgttgctg tttctggccc tcacagccgg cattccctgc cgcggcttgg gcgatgacgc
     3721 cgaaccgctc cacgctcgga tcgatcgtct catcgacgcg cagacgggcg ggccgttggc
     3781 agatgattcc tcggatgtag agttcctgcg tcgcatttat ctcgatgtgg taggtcgcgt
     3841 accgtcggct gaggaaacgc gggccttttt ggcggattcg aatgccgaca agcgggctgc
     3901 gctg
//
LOCUS       DASUGB010000245         5243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_444708, whole genome shotgun sequence.
ACCESSION   DASUGB010000245 DASUGB010000000
VERSION     DASUGB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444708"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFV87_04740"
     CDS             complement(<1..428)
                     /locus_tag="VFV87_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5103091.1"
                     /translation="MNMHIRWRGFELPSIVNCDAASLTGTYGKFTAEPFERGFGVTIG
                     NSLRRVLLSSLEGSSVTQIKIRGAQHEFTTIPGVLEDVTDIVLNVKSLVVKNHSDSTR
                     VITVEKNTTGVVTGADVQVDSDVEIINKNHVLATLTDNVP"
     gene            complement(539..928)
                     /gene="rpsK"
                     /locus_tag="VFV87_04745"
     CDS             complement(539..928)
                     /gene="rpsK"
                     /locus_tag="VFV87_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5103092.1"
                     /translation="MAQAATTKKKKIRRNVTAAIAHVKATFNNTVVTITDTKGDALCW
                     ASAGTCGFKGSRKSTPFAGQCAGQQAAEKAAKYGVKELEVRVKGPGSGRESAITALQT
                     AGMTVKIIEDVTPIPHNGCRPPKRRRV"
     gene            complement(971..1354)
                     /gene="rpsM"
                     /locus_tag="VFV87_04750"
     CDS             complement(971..1354)
                     /gene="rpsM"
                     /locus_tag="VFV87_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5103093.1"
                     /translation="MPRLLGVDIPNDKPIVISLRYLYGVGDQIARELCHKAGIETHKH
                     ARDLHEEEVSRLTTILEKDYVVEGQLRRQVAQDISRLKDIKCYRGLRHRMSLPVRGQR
                     TRTNARTRKGPKKTVAGKKGVKDLR"
     gene            complement(1398..1511)
                     /gene="rpmJ"
                     /locus_tag="VFV87_04755"
     CDS             complement(1398..1511)
                     /gene="rpmJ"
                     /locus_tag="VFV87_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5103094.1"
                     /translation="MKVRTSVRKICEHCKIVRRKGRVYVVCTNARHKQRQG"
     gene            1776..3065
                     /locus_tag="VFV87_04760"
     CDS             1776..3065
                     /locus_tag="VFV87_04760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5103095.1"
                     /translation="MLRTRAVPFLALTVLLLLVPLLRAADPVLKAGDRVAIIGDSITE
                     QKLYTKYMECYLLACSGVPDLAVMQYGWSGERANGFADRLENDLAGFKPTIATTCYGM
                     NDGSYQPYNDQIGQAYEANMRRVLAGLAKLGVRTTAVGSPGAVDQNFFRPGQMMGDQP
                     AHVAYNDNLAHLRDIAKQLAAETKQPFANVHDAMYQSMLKAQEKLGSKYDVCGGDGFH
                     PGPGGQLIMAYAFLKALGCDGDIGTITYDAKENKAAGSAGHKVTLAGDKINVESTRWP
                     FVFEGDGKTSGSNRSITPFLPFNDDLNRLTLKVTGLGTPKGVVTWGDQTKEFPAEKLA
                     AGINLAAEFEKTPFDAKFAELSQAVAQKQAFETFLIKQVVTGFRFYPAEVKSDPAVQS
                     ALATLADRLHARQQSLHTAARATITPVSHTLTIAPLP"
     gene            complement(3072..3302)
                     /locus_tag="VFV87_04765"
     CDS             complement(3072..3302)
                     /locus_tag="VFV87_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103096.1"
                     /translation="MQIERAILNEDDGLLGYIVSPEFRGKDSYARQTMIFDALSASEA
                     KLTLPEIRKVVGVVAFTPEEFAVHGPELQRSA"
     gene            complement(3323..3811)
                     /locus_tag="VFV87_04770"
     CDS             complement(3323..3811)
                     /locus_tag="VFV87_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX5103097.1"
                     /translation="MQFIADHYYRAALERIEDARLLYSQERFAFSMYVSGLAVEGLLR
                     AFYWRKNPVFDARHDLAQLFKESGIAGAEEQRLTRRGLDSIEITKALTELFAARDTVA
                     RLWSNDYRFAPESQVRARLRALGERQNKRGNQVKPSALALYNSAKLLIDRSTVIWTSG
                     KK"
     gene            complement(3826..>5243)
                     /locus_tag="VFV87_04775"
     CDS             complement(3826..>5243)
                     /locus_tag="VFV87_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX5103098.1"
                     /translation="PKTAKNIVKKGLLAAEAREAARKAKEVLRSRKGVLGSGSLPGKL
                     RDCLSRNMEECELYLVEGDSAGGSAEGGRLREFQAILPLRGKIINAYKSREDKVLDNA
                     EVQAMIQAIGTNIGADQDMSRLRYNKIIIMTDADVDGSHIRTLLLCFFYRQMYQLVAT
                     EHVYVAQPPLYRVSKGKAKPYYVQTDEEMKEQLLGRGVDNSELDLFDGRKITGEKMKR
                     LAGLLAGMDEALIALERRGISLRDHSGRLVAGKLPIFHVYLGRDEHWFATRSELDTFL
                     SQQEEAKGGELAVSTSATESGAGILPAEKTADHTKPGANGDATTADSAPKASRLHITE
                     LHEVRTINQGLADLKNDFGFDLDMLIPQERTGSDQPRYVLRRGEHEVPLEDLRGLAAA
                     IREMGQKGLTVTRFKGLGEMNAEELRETTLDPANRTLLRVKMEDVAEADNMFRVLMGD
                     KVEPRREFIEKHALEVRNLDV"
BASE COUNT          882 a         1725 c         1594 g         1042 t
ORIGIN      
        1 aaggggacgt tgtcggtgag cgtggccagg acgtgattct tattgatgat ctcgacatcc
       61 gaatcgacct ggacgtcggc cccggtcacg acgcccgtgg tgttcttctc gacggtgatc
      121 acgcgggtcg aatcgctatg gttcttcacc accagcgact tgacgttgag gacgatgtcg
      181 gtcacatcct ccagcacgcc ggggatcgtg gtgaattcgt gctgggcgcc gcgaatcttg
      241 atttgcgtaa ccgagctccc ttccaaactg gagagcagga cgcgccgcag gctgttgccg
      301 atggtcacgc cgaagccccg ctcgaagggc tcggcggtga acttgccgta cgtgccggtc
      361 agcgatgcgg cgtcgcaatt gacgatgctc ggaagctcaa acccgcgcca acgaatgtgc
      421 atattcaatc tccaaaaggg cgtcggtacc tggtagccgc actcgccaga gtgcggggaa
      481 ttcgccacga tccccgcgtt ctggcgaacg cggctacttt cgacgggcgt ttgctttcct
      541 acacgcgccg ccgcttgggg gggcggcagc cgttgtgggg aatcggtgtg acatcctcga
      601 tgattttgac cgtcatgcct gcggtctgca gggcggtaat ggcgctctcg cggccggagc
      661 ccgggccttt cacccgcacc tccagctcct tgacgccata tttcgcggcc ttctccgccg
      721 cttgctggcc ggcgcattgg ccggcaaagg gcgtgctctt gcggctcccc ttgaagccgc
      781 acgtgccggc gctggcccag cacaaggcgt cgcccttggt atcggtgatg gtgaccaccg
      841 tattgttgaa cgtcgctttg acgtgggcga tcgctgccgt cacgttgcga cggattttct
      901 tcttcttggt tgttgctgct tgagccactg ccactcgctc ccgcgatctg attcctgctg
      961 aacgaaactc ctaccgcaaa tccttcacgc ccttcttgcc ggctaccgtc ttcttcggtc
     1021 ctttccgcgt gcgggcgttg gtccgcgtcc gctgcccgcg gacgggcagg ctcatgcggt
     1081 gccgcaagcc gcgatagcac ttgatgtcct tcaggcgact gatgtcctgc gcgacttgcc
     1141 gccgaagctg accttcgacg acgtaatcct tctccaggat cgtcgtcagg cgcgaaacct
     1201 cttcttcgtg caggtcgcga gcgtgcttgt gcgtctcgat ccccgccttg tggcacagtt
     1261 cccgcgcaat ctgatcgccc acgccataca aatagcgcag cgaaatcacg atcggcttat
     1321 cgttcggaat gtcaacgccc agcaagcgag gcataagtcg cccttggttc tgcggtgctt
     1381 gtttcttgga tcttccgcta cccctgccgc tgcttgtggc gggcgttagt gcagaccacg
     1441 tacacccgcc ccttgcggcg cacaatctta cagtgctcgc agatcttccg cacgctcgtc
     1501 cgcaccttca tagcactacc caccccggat ggagtaaagc gcgccagtat aaccctttgc
     1561 gacgaattcc gtaaaggggg cccctggcga ttgattcagg tcgtctgacc gtgccgagtc
     1621 gggagtttcc gggcgaggaa gggctgcttg tgttacaaca gcgtgatctt cgccctgctt
     1681 ctcccgtggt cagatgccct gcaggttccc ctatctgact gggagacggc tggggtgaga
     1741 tacaacatct tccttgcaac gtcgggaaat caatcatgct ccgcactcgc gccgtcccat
     1801 tcctcgccct cacggtcctc ctgctcctcg tcccgctgct tcgcgcggct gatccggttc
     1861 ttaaagccgg cgatcgcgtg gcgatcatcg gcgattcgat taccgagcag aagctctata
     1921 cgaagtacat ggagtgctat cttctcgcct gctcgggcgt gccggacctg gcggtgatgc
     1981 agtacggctg gagcggcgag cgggcgaatg gctttgccga ccggctggag aacgacctgg
     2041 cgggcttcaa gccgacgatc gccaccacct gctacggcat gaacgacggc agctatcagc
     2101 cgtacaacga ccagatcggc caggcgtacg aagcgaacat gcgccgcgtg ctggccgggc
     2161 tggcgaagct gggcgtgcgg accacggcgg tcggctcgcc cggcgcggtc gatcagaact
     2221 tcttccgccc cgggcagatg atgggggacc agccggccca tgtcgcctac aacgacaacc
     2281 ttgcccacct ccgcgatatc gccaaacagc tcgccgccga gaccaagcag cccttcgcca
     2341 acgtccatga cgcgatgtac cagtcgatgc tgaaggccca ggagaagctt ggctccaagt
     2401 acgacgtctg cggcggcgac ggcttccacc ccggaccggg cggccagctc atcatggctt
     2461 acgcgttcct caaggccctc ggctgcgatg gcgatatcgg caccatcacc tacgacgcca
     2521 aggaaaacaa agccgccggc agcgcggggc acaaagtcac gctggccggc gacaagatca
     2581 acgtggaaag cacccgctgg ccgttcgtct ttgaggggga cggcaagact tcgggcagca
     2641 accgcagcat cacccccttc ctgccgttca acgacgacct gaaccgcctg acgctcaagg
     2701 tcactgggct aggcacgccc aagggggtcg tcacctgggg cgaccagacc aaggaatttc
     2761 ccgccgaaaa gctcgccgcc ggcatcaacc tggcagccga gttcgagaag acgccgttcg
     2821 acgcgaagtt cgccgagctc tcgcaagccg tcgcccagaa gcaggccttc gagacgttcc
     2881 tgatcaagca ggtcgtcacc ggcttccgct tctatccggc cgaagtcaaa tccgaccccg
     2941 ccgtccaatc ggccctcgcc accctcgccg accgcctcca cgcccgccag caatccctcc
     3001 acaccgccgc ccgcgccacc atcacgccgg tgagccacac cctcaccatc gccccgctgc
     3061 cgtaaggcaa ttcatgccga ccgctgcagt tccggcccgt ggacagcgaa ttcctcgggc
     3121 gtgaaggcga cgacgcctac gactttgcga atctccggga gcgtaagctt tgcctcggat
     3181 gcgctcaggg catcgaagat catggtttgc cgcgcatagg aatccttgcc gcgaaactcc
     3241 ggcgatacga tgtagcccaa caagccatca tcttcgttaa ggatcgcccg ttcgatctgc
     3301 attgccgagc gaagcgcccg aactactttt ttccggaggt ccatataacc gtacttcgat
     3361 caatgaggag ttttgctgaa ttatacagcg ccagggcgct cggcttgacc tgatttccgc
     3421 gcttgttctg gcgctcgccc agagcccgaa ggcgggcacg aacctgcgat tctggtgcaa
     3481 aacggtaatc gttgctccag agcctggcga cggtgtcgcg ggctgcgaac agctccgtga
     3541 gcgctttggt gatttcaatg gagtccaagc cgcgacgcgt gagtcgctgt tcttcagccc
     3601 cggctatccc gctctccttg aagagctggg ccaaatcgtg gcgggcgtca aacacgggat
     3661 tcttccgcca gtagaaggcc cgcaaaaggc cctcaaccgc taagcccgac acgtacatgc
     3721 tgaacgcaaa ccgctcctgc gaatagagca gccgggcgtc ttcgatccgc tccagcgcgg
     3781 cacggtaata gtggtcggcg atgaattgca tggccggcca ggattctaca catccaaatt
     3841 ccgcacctcc agcgcgtgtt tttcgatgaa ctcgcggcgg ggttcgactt tgtcgcccat
     3901 caggacgcgg aacatgttgt cggcttcggc gacgtcttcc atcttgacgc ggagcagcgt
     3961 gcggttggcg gggtcgagcg tcgtctcgcg gagctcctcg gcgttcatct cgcccagacc
     4021 tttgaagcgg gtgaccgtca gccccttctg gcccatctcg cggatcgcag ccgccaggcc
     4081 ccgcaggtcc tccagcggca cctcgtgctc gccccggcgg aggacgtacc gcggctggtc
     4141 gctgccggtc cgctcctgcg ggatgagcat gtcgaggtcg aagccgaagt cgttcttgag
     4201 gtcggccagg ccctggttga tggtgcggac ctcgtgcagc tcggtgatgt gcaggcgcga
     4261 ggccttggga gcggaatcgg cggtggtggc atcgccgttg gcgccgggct tcgtgtggtc
     4321 tgccgtcttt tcggcaggca ggatgccggc cccgctctcg gtggcgctgg tgctgacggc
     4381 cagctcgccc cccttggcct cttcctgctg ggagaggaac gtgtcgagct cgctgcgggt
     4441 cgcgaaccag tgctcgtccc ggcccagata gacgtggaag atcggcagct tgccggcgac
     4501 gaggcggccg gagtggtccc gcaggctgat gccgcggcgc tccagggcga tcagggcctc
     4561 gtccatgccg gcgagcaggc cggccaggcg cttcatcttc tcgccggtga tcttgcggcc
     4621 gtcgaacagg tccagctcgg agttatcgac accgcggccg agcaactgct ccttcatttc
     4681 ctcgtcggtc tgcacgtaat acggtttggc cttccccttg ctcacgcgat acagcggcgg
     4741 ctgggccaca tagacgtgct cggttgccac aagctggtac atctgccggt agaagaagca
     4801 gagcaagagc gtgcggatgt gcgagccgtc cacgtcggca tcggtcatga tgatgatctt
     4861 gttgtagcgc aggcggctca tgtcctgatc ggcgccgatg ttggtgccga tcgcctggat
     4921 catcgcctgg acctcggcgt tgtcgaggac cttgtcctcg cgcgacttgt aggcgttgat
     4981 gatcttgccc cgcagcggca ggatcgcctg aaactcgcgc agccggcccc cttcagcact
     5041 tccaccggcg gaatcgccct cgaccaggta cagctcgcac tcctccatat tccggctgag
     5101 gcagtcgcgc agcttgccgg gcaacgagcc gctgccgagc acccccttgc ggctgcggag
     5161 cacctccttg gccttgcggg cggcttcgcg ggcctcggcg gcgaggagcc cctttttgac
     5221 gatgttcttg gccgtcttgg ggt
//
LOCUS       DASUGB010000246         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_862248, whole genome shotgun sequence.
ACCESSION   DASUGB010000246 DASUGB010000000
VERSION     DASUGB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862248"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..735)
                     /locus_tag="VFV87_04780"
     CDS             complement(157..735)
                     /locus_tag="VFV87_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103099.1"
                     /translation="MNIDSGSSAFEARRRALEEAFFQKRDQELLEKLRGELSALEESR
                     KLAHVSGIVQEKVLMDLVKAGLTAETLAAVVTIPMVEVAWADGAVSASEKAAVLNAAA
                     GQGIHPGTAPHDLLERWLVDRPDSRIVAAWKEYVRELAKLMPAETIAQMHKNVLDRCV
                     RVAEAAGGFVGIHRISKVEQATIDDFANVFKD"
     gene            917..1468
                     /locus_tag="VFV87_04785"
     CDS             917..1468
                     /locus_tag="VFV87_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5103100.1"
                     /translation="MTHPWHDVTPGLDLPGEFISVIEIPRGSSVKYELDKATGLLKLD
                     RILYSAVHYPANYGFIPQTLAEDDDPLDVLVLCQEAVDPLTLVHARAIGLMTMIDSGK
                     KDHKILAVAKHDPEFNSFQEASELPIHRLALLRRFFQDYKQLEGKTVEVDDFQPAAFA
                     MPIIEDALQRYSTQRRKGFAAKL"
     gene            1551..2228
                     /gene="rsmG"
                     /locus_tag="VFV87_04790"
     CDS             1551..2228
                     /gene="rsmG"
                     /locus_tag="VFV87_04790"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5103101.1"
                     /translation="MATDPPPGPPDPDSLPAALARHGITLPAGQIALLDRYARLLWEW
                     NEKFNLTRHTDYEKFVSRDLVDSLQLAALLLPGETVLDVGTGGGVPGIVLAIVRPDLQ
                     VTLLDSVQKKIRAVSEITAALSLPVETVCARVEEHLEDARYDALVFRAVGPLWELLRW
                     CKPHWNMIGRLLVVKGPKWPEERHEAAHRGLLRALELKLAGEYPLAGTESQSVILKLW
                     PKGRKER"
BASE COUNT          493 a          926 c          754 g          502 t
ORIGIN      
        1 gtccaaccgc gcagggtcag gcaaggccat cccccgtcaa ccgtagcatg cggtttggca
       61 acaggtggcg atttggcaac gtcgttcgtc gcggaaaaac aacgggccgg cagcggatgc
      121 gccggatgta gcgactgtac cgcgctcgcc aaggcgtcag tctttgaaga cgttggcgaa
      181 gtcgtcgatc gtcgcctgct cgaccttcga gatgcgatga atgcccacaa agccgccggc
      241 cgcctcggcc acgcggacgc agcggtcgag cacgttcttg tgcatctggg cgatcgtctc
      301 ggccggcatc agcttcgcga gctcgcggac gtattccttc caggcggcga cgatccgcga
      361 atcgggccga tcgaccagcc agcgctccaa caggtcgtgc ggcgccgtgc cgggatggat
      421 tccctgcccc gcagccgcgt tgagcacggc ggccttctcg ctcgcgctca ccgccccgtc
      481 agcccacgct acttcgacca tcggaatggt gaccaccgcg gcgagcgtct ccgccgtcag
      541 gccggctttg acgagatcca tcagcacctt ttcctgcacg atcccggaga catgcgcaag
      601 cttccgcgat tcctcgagcg cggacaattc gccgcgcagc ttttccagca actcctgatc
      661 gcgcttctga aagaaggcct cctccagcgc gcggcggcgg gcctcaaaag cactgctgcc
      721 cgaatcgatg ttcatggcgt cgctccgtcg gggatctagg tgtacttagg gttagcctac
      781 tcattctatc gacggccgct gctttggcct acgcctccat ccgactcatc atattcccga
      841 aacgcccggc gctgatacga ttgatgctcg ccactccgca ttccgcattc cgcactccgc
      901 attccgcact tggcccatga cccacccctg gcacgatgtg actcccggcc tcgacctgcc
      961 cggcgagttc atttccgtca tcgaaatccc ccgcggcagc agcgtgaaat acgagctcga
     1021 caaggccacc ggcctgctca agctcgatcg cattctctac tccgcggtcc actacccggc
     1081 caactacggc ttcattccgc agacgctggc cgaggacgac gacccgctgg acgtgctcgt
     1141 cctctgccag gaagcggtcg atccgctgac gctcgttcac gcccgggcca tcggactgat
     1201 gacgatgatc gacagtggaa agaaagacca taagattctc gccgtcgcca agcacgatcc
     1261 agaattcaac agcttccagg aagcgagcga gctgccgatt catcggctgg ccctgctcag
     1321 gcggttcttt caggattaca agcagctcga aggcaaaacg gtcgaggtcg atgacttcca
     1381 accggccgcc tttgccatgc cgatcattga ggacgccctg cagcgctaca gcacccagcg
     1441 gcggaaaggc ttcgcggcaa agttgtaaat gtcgcctttc gctccgcgaa agcgcgttcg
     1501 ttcgcggagc gaacgacgac aatccagcga tctggtgaga taatccgcgc atggcaaccg
     1561 atcctccccc aggtccgccg gaccctgatt cactccccgc cgctctcgcc cgccacggca
     1621 tcacgctgcc cgctggacaa atcgccctcc tcgaccgcta tgcccgcctg ttgtgggagt
     1681 ggaacgaaaa attcaacctc acgcggcaca cggactacga aaaattcgtc agccgcgatc
     1741 tggtcgattc gctgcagctt gccgccctgc ttttgccggg cgagacggtg ctggatgtcg
     1801 gcaccggcgg cggcgtgccg ggcatcgtgc tggcgatcgt ccgcccggac ctccaggtga
     1861 cgctgctcga cagtgtgcaa aagaaaatcc gcgccgtttc tgaaatcact gccgctctca
     1921 gtctgccggt cgaaaccgtc tgtgcccgcg tcgaagagca cctcgaagac gcccgctacg
     1981 acgccctcgt cttccgcgcc gtcggccccc tctgggagtt gctccggtgg tgcaagccgc
     2041 actggaacat gatcggccgg ctgctggtcg tcaaaggccc caagtggccc gaagagcgcc
     2101 acgaagccgc ccaccgcggc ctgctgcgag ccctcgaact caagctggcc ggcgagtacc
     2161 cgctcgccgg caccgaatcg caaagcgtga tcctcaagct ctggccgaag gggcggaagg
     2221 aacgatgagt ggtattttgt gttttgtgtt tggtgttttg acttgaaata caaaacacaa
     2281 aacaccgccc cgcatttgac tcccaccaaa tcccgaccta gtgtttcgat acgcccgctc
     2341 gcaacgctcg ttgcggcggg ttttcaacaa tcccgacttt ccacccggcg agtcaactat
     2401 tgccggacct cggaggggtt aggggttgct cttttgaatg gaactgcggg gccgccggtt
     2461 cgccggcagc cccgcatgac ttttcccaca tctccgcacc aacctgtttg gtccagcgcg
     2521 caagcggcct gtccccgctg gcagcgtccg gtttccatat ccaagtcgaa tttcccggac
     2581 ctgcttttgg gactgccgca tgcaaacact caccctcgac gaactactgc ccaacatccg
     2641 gctggcggct cgccgtgccg ccagccgcgg cgcgg
//
LOCUS       DASUGB010000247         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1834654, whole genome shotgun sequence.
ACCESSION   DASUGB010000247 DASUGB010000000
VERSION     DASUGB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834654"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="fliM"
                     /locus_tag="VFV87_04795"
     CDS             <1..840
                     /gene="fliM"
                     /locus_tag="VFV87_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX5103102.1"
                     /translation="QMRALEALHEGFGRNFGAALSGYLRTIIEVNVAHIEQLTYSEFI
                     HSLPNPTCFNLLKADQLDGQLCLEISPLIIYPIIDRLLGGSNADLFIPQRPLTQIEQR
                     LVQRITDRATHHLSEAWSNLTPITFKVEDFESNPQLVQIVPPNEVVVLISFELTLGEI
                     RGMLNLCIPFNAIERNAGKLAANTWATYGRLSADQKTRSQVARRLDQSEVELVVTLAE
                     STISTGDLLGLRVGDIITTDKDLHQPLDVEIAGAPKFHASPGAFKGRKAIEIQSPPTP
                     PVH"
     gene            958..1788
                     /locus_tag="VFV87_04800"
     CDS             958..1788
                     /locus_tag="VFV87_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103103.1"
                     /translation="MSAVLLISVLAAVGQGPAVEEPASAKAVEVFRAEAAEYVVRLQS
                     RQNEKLALEKEPVLRWSNPARTGEDGATFIWTLDGRPEMIGTVFTYLYNDKINRKHEY
                     HSLATEPLAAEFRGKEVWSPKAAGVKFAPVAGAPLPAGSSRLRLTQIKALARDFSASM
                     KDEEGESYQLRLLTQPLFRYEPEDKRVLDGALFAFSLGTDPEVLLMIEARPVASGNQW
                     QFALARFHYVDLKGFYKDREVFHAEPLLNINNLNLGDSQYRDSVYATYHAQRNVPVEE
                     "
     gene            1899..2387
                     /locus_tag="VFV87_04805"
     CDS             1899..2387
                     /locus_tag="VFV87_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5103104.1"
                     /translation="MDARQAILANLASADFTVDGYLADIQPAEMLARAVPGSNHLAWQ
                     IGHLIQSERYLAERCQPGSICPLPAGFEARHKKETAGSDNPADFLSKEEYQRIRGQVR
                     ADVSKLIESLKPEDLDKPVDKVPPFLKTVGQTLLFLGPHWLMHAGQWAVIRRKLGRPP
                     LF"
BASE COUNT          512 a          786 c          779 g          485 t
ORIGIN      
        1 cagatgcggg cgctcgaggc cctgcacgaa ggattcggcc ggaacttcgg cgccgccctg
       61 tctggttacc tgcgcaccat catcgaggtc aacgtcgcgc acatcgagca gctgacgtat
      121 tccgagttca tccactcgct gccgaacccg acctgcttca acctgctcaa agccgatcag
      181 ctcgacggac agctgtgttt ggagatcagc ccgctgatca tctatccgat catcgatcga
      241 ttgctcggcg gatcgaatgc agacctgttc atcccgcagc ggccgctgac gcagatcgag
      301 cagcgcctgg tacagcgcat cacggaccgt gcaacgcatc acctctccga ggcatggagc
      361 aacctcacgc ccataacgtt caaggtcgag gatttcgaga gcaatccgca gctcgtgcaa
      421 atcgtgccgc ccaacgaagt cgtggtgctc atcagcttcg agctcacgct cggcgaaatc
      481 cgcggcatgc tcaacctgtg catcccgttc aacgccatcg agcgcaacgc cggcaaactt
      541 gcggccaaca cctgggccac gtacggccgg ctgtccgccg accagaagac ccgctcccag
      601 gtcgcacgac ggctcgatca atcggaagtc gagctggtcg tcacgctcgc cgaatcaacg
      661 atttccaccg gcgaccttct gggcctccgc gtcggcgaca tcatcacgac cgacaaagac
      721 ctccaccaac cgctcgatgt cgaaatcgcc ggcgctccga agttccacgc ctcgcctggc
      781 gctttcaagg gccgcaaagc gatcgaaatt caatcgccac cgacgccacc cgtccattag
      841 tctgctagtc tgccgccgca gaatgagcgt tgtccgggcg gatgggacga gcctgacgct
      901 atggcctttt cagcggcgtg gcagtagttt agacataccg tcttagtccg aggcaatatg
      961 agcgcggtac tgttgatttc tgttttagct gccgtcggcc aaggaccagc tgtggaagag
     1021 ccggcgtcgg ccaaagcggt cgaagtgttc agggccgagg cggcggagta tgtcgtgcgg
     1081 ctgcagagtc ggcagaacga gaagctggcg ttggaaaagg agccggtgtt gcggtggagt
     1141 aatccggcgc ggaccggcga agacggggcg acgtttatct ggacgctcga tggccggccg
     1201 gaaatgatcg gcacagtttt tacctatctc tacaacgaca agatcaaccg caaacacgag
     1261 taccactcct tggcgaccga acctctggcg gcggagtttc gcggcaagga agtctggtcg
     1321 ccgaaggcag ccggcgtgaa atttgccccg gtcgccggcg cgccgctgcc tgccggcagc
     1381 tcgcggctca ggctgacgca gattaaggcc ctggctcgcg atttttcggc cagcatgaag
     1441 gacgaagagg gcgaatcgta tcagctgcgc ctgctcaccc agccgctatt ccgctacgag
     1501 ccggaggaca agcgggtact ggacggggcg ctatttgcat tttcgctcgg gaccgatccg
     1561 gaagtgctac tgatgatcga ggcgcggccg gtggccagtg gaaatcagtg gcaatttgcg
     1621 ctggcccgtt tccactacgt cgatctaaaa gggttttaca aggaccggga agtatttcat
     1681 gccgagccgc tgctgaatat caataacctc aatttgggtg actcgcagta tcgcgacagc
     1741 gtatatgcga cgtatcacgc gcagcggaat gtgcccgtgg aggaataatc attggcgagg
     1801 atcgggaatc aaactctcga ggcgagcagt tgtgctaaac tgcacgcgtc gctggctcac
     1861 gcaccgcctg catattctga accgcaccgg gaggacacat ggacgccagg caagcgattc
     1921 tcgccaatct cgcctccgcc gatttcaccg ttgatgggta tctggcagat attcagccgg
     1981 cggagatgct cgcacgggcc gtgccgggct cgaaccattt ggcgtggcag attgggcact
     2041 tgattcagag cgagcggtat ctggccgagc gctgccagcc aggatcgatc tgtccgctgc
     2101 cggcgggctt tgaagcgcgt cacaagaagg agaccgctgg gagcgacaat ccggccgact
     2161 ttctgagcaa ggaagagtac cagcgaattc gcgggcaagt acgggccgac gtgagcaagc
     2221 tcatcgagtc cctgaagccg gaggatctcg acaagccggt ggacaaagtg ccgccgttct
     2281 tgaagacggt cggccagacg ctcctctttc tcggtccgca ttggttgatg cacgcgggac
     2341 aatgggccgt gatccgccgc aagcttggcc gtccgccgct gttctgatgg agcggggtcc
     2401 gcttcccgtg gcgcggcgaa taatgtcttg gcccattgcc gagtttgcat gcgcgatgcg
     2461 ggcaccgctg cttttgtgcg ctcacggtcc ggccgcctcc cgccccttgt caagcctgcg
     2521 acagccgccg atgctgttgg ctcattaaac cttgccccca gc
//
LOCUS       DASUGB010000248         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_166637, whole genome shotgun sequence.
ACCESSION   DASUGB010000248 DASUGB010000000
VERSION     DASUGB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166637"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFV87_04810"
     CDS             complement(<1..675)
                     /locus_tag="VFV87_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5103105.1"
                     /translation="MPRRVVITGIGVTSPLGNSADKLWNFLSSGTSGVRVLQSLPGEV
                     LPSRYGGEAIDFTGTIEDFGPLEKTLGRTIKKALKMMCREMQMGVAVAQLALTDSKLP
                     LATIDRDRVGVIYGSDYMLTVPEEFVAGMRNCLDAEGRLHVSEWGEKGRPTVEPLWLL
                     KYLPNLPASHISIFNDLHGPNNSLTMREASSNYAVAEAYHTILRGHADAMIAGACGTF
                     VHPNRTL"
     gene            860..>3260
                     /locus_tag="VFV87_04815"
     CDS             860..>3260
                     /locus_tag="VFV87_04815"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5103106.1"
                     /translation="MLADGAAQAAEIAVLQPGNWDELVPAGKEVDAIYGDYVLRNDQI
                     VAVIAQPLPTRNANMTVRGVGAAIIDLTRRDEPNDQLSAYYPGAGRYPFVSPDRVRTS
                     LYGRAYQLKPGERERGDPVSLEIAADAVEGKPSLTVRYTLTSDAEFLLVETIYSNPGA
                     APLEAEPADSIRADRTFAFGTDPATSLFWADDEWFRQCYGVVVPGYEIKPTSERATLL
                     QLVKDGSTKFTLQPGESQTIARKIFPASSLLGVRGMASELAGQKVASAEVGVADLTRV
                     QAKVALTKDGRAYAAGWTDVLGKLEFLLPEGTYESEVTSVDGRRAKGKLTSGQEKRHI
                     VKLALPSYVVAFITDANGRPSPAKVSFTGKDGTKDPDWGPDSGEIAVKNVYYTHNGLF
                     KQAIAPGKYDVIVSYGPEHDAVFKSIDVPAGGDAVLKATLKRSVDTSGWISADFHSHS
                     SPSGDNTSSQLGRVLNLLCEHVEFAPCTEHNRIDTYVPHLQHLQVEHLMATCTGMELT
                     GGPLPVNHQNAFPLLMKPRTQDGGAPVTDIDPVIQIERLALWDNQSDKLVQMNHPNLP
                     QILGDRNEDGQPDAGFERMFGFVDVIEVHPPQGVFDPPTKGEDGKLTRNPIFHWLQML
                     NRGYRISAVINTDSHYNWHESGFYRNFIKSPTDDPAKIDTMEMVHASERGNLVVSTGP
                     FLTVEAVAAALDGKIVRAGPGDDFAPTEGQCSLRIRVQCPNWFDINRVQVFLNGRPEQ
                     DLNFTRRTTPNRFRDETVRFDHSIPLELKGDTHVIVATIGEGLTLGPVMGPNYGGKLP
                     PV"
BASE COUNT          634 a         1038 c         1032 g          556 t
ORIGIN      
        1 gagcgtgcgg ttgggatgca cgaaagtgcc gcacgcgccg gcgatcatcg catcggcgtg
       61 gccgcggagg atcgtgtgat aggcctcggc aaccgcataa ttgctggacg cctcgcgcat
      121 cgtgagcgaa ttgttcggcc cgtgcaggtc gttgaaaatc gaaatgtggc tggccggcag
      181 attgggcagg tacttcagca gccagagcgg ctcgacggtc ggccggccct tctcgcccca
      241 ttcgctcacg tgcagccgcc cttcggcatc gaggcaattc cgcatgccgg cgacgaactc
      301 ctcgggcacg gtgagcatgt aatccgagcc gtagatgacg ccgacgcggt cgcggtcgat
      361 cgtcgccagc gggagcttcg aatcggtgag cgcgagctgg gcgacggcca cgcccatctg
      421 catctcgcgg cacatcatct tgagggcttt tttgatcgtc cgcccgagcg tcttttccag
      481 cgggccgaag tcctcgatcg tcccagtgaa atcgatcgcc tcccccccgt agcgactggg
      541 gagcacttcg cccggcaggc tctgtagcac gcgcaccccg ctcgtgccgc tagaaaggaa
      601 gttccaaagc ttgtcagccg aattccctag cggagaggtg accccaatcc cggtgatgac
      661 gacgcggcgt ggcatgaata agcagaatcg ggtggcggac atcggcagac cagaatgtct
      721 gccccactgg ctgtatttct ggtggtggag gtcttattgt attttcgtcg gcgaaaggcg
      781 tcgaggcggc ggaactgtcc tcagggggtg gatgatgcga tctcagctta cttggtcaat
      841 tggactgttc attgttgcca tgcttgcgga cggcgcggct caggcggcgg aaatcgccgt
      901 gctgcagccg ggcaattggg acgagctggt tcccgccggc aaggaggtgg acgcgattta
      961 tggcgactac gtgctgcgga acgaccagat cgtggccgtc atcgcccagc cgctgccgac
     1021 gcggaacgcg aacatgaccg tccgcggcgt cggggcggca atcatcgacc tgactcgccg
     1081 cgacgagccg aatgaccagc tcagcgcgta ttatcccggc gcggggcgct atccctttgt
     1141 ctcacctgat cgcgtgcgca cgagcttgta cggacgggcg taccagctaa agccgggcga
     1201 gcgcgagagg ggcgatccgg tcagcctgga gattgccgca gacgcggtcg agggcaagcc
     1261 tagtctgacg gtccgataca cactgactag tgacgcagag tttctgttgg tcgaaacgat
     1321 ttattccaac cccggcgcgg cgcccctgga agcggagcca gccgattcca tccgcgccga
     1381 ccgcacgttc gctttcggca cggacccggc gacgagtctc ttttgggctg acgacgaatg
     1441 gttccgccag tgttacggcg ttgtcgtgcc cggctacgag atcaaaccaa ctagcgagcg
     1501 ggccacgcta ctgcagcttg taaaggacgg cagtacgaaa ttcacgctgc agcccggcga
     1561 gtcgcagacg atcgcccgca aaatctttcc ggcgagcagc ctccttggcg tccgcggaat
     1621 ggccagcgaa ttggccggcc agaaagtcgc cagtgccgag gtcggcgtcg ccgatttgac
     1681 tcgtgttcag gcaaaggttg cgctgacgaa ggatggcagg gcgtacgcgg ccgggtggac
     1741 ggacgtcttg ggtaaactcg aattcctgct gccggaaggg acgtacgaga gcgaggtgac
     1801 ttcggttgat ggccggcggg ctaaaggcaa gctcacgagc gggcaggaga aacggcacat
     1861 cgttaagctc gctctgccaa gttacgtcgt tgcgtttatc accgacgcga acggaaggcc
     1921 aagtcccgcc aaggtctcgt tcaccggcaa ggacggcacg aaagaccccg actgggggcc
     1981 cgattccggc gagatcgcgg tcaagaacgt ctattacacg cacaacggat tgttcaagca
     2041 ggcgatcgcg ccgggcaagt acgacgtgat cgtcagctat ggcccggagc acgatgccgt
     2101 cttcaagagc atcgacgtgc ccgccggcgg cgatgccgtg ctcaaggcga ctctcaagcg
     2161 ctcggtcgat acgagcggct ggatcagtgc cgattttcac agccactcca gcccttcggg
     2221 cgacaacacg tcgagccagc tcggccgcgt gctcaacctg ctttgcgagc acgtcgagtt
     2281 cgccccctgc accgagcaca accgcatcga cacctatgtg ccacacctcc agcatctgca
     2341 ggtcgagcat ctcatggcca cctgcaccgg catggagctg accggcggcc cgctgccggt
     2401 gaaccatcaa aacgccttcc cactgctgat gaagccgcgg acgcaagatg gcggcgctcc
     2461 ggtgaccgat atcgacccgg tcatccagat cgagcggctc gccttgtggg acaaccagag
     2521 cgacaagctc gtgcagatga accatccgaa cctcccgcaa atcctcggcg accgcaacga
     2581 agacggccag cccgacgccg gtttcgagcg gatgttcggc ttcgtggacg tgatcgaggt
     2641 ccacccaccg cagggcgtct tcgatccgcc gaccaagggg gaggacggca agctgacgcg
     2701 gaacccgatc ttccactggc tgcagatgct caaccgcggc tatcggattt cagccgtgat
     2761 caacaccgac tcgcactaca actggcacga atcgggcttc tatcgaaatt tcatcaagag
     2821 cccgaccgac gacccggcga agatcgacac gatggagatg gtccacgcgt cggagcgcgg
     2881 caacctcgtc gtcagcaccg ggccgttcct caccgtcgaa gccgtcgccg ccgcactcga
     2941 cggcaagatc gtgagggccg ggccgggcga cgacttcgcg cccacggaag gacagtgctc
     3001 gctgcgaatc cgcgtgcagt gccccaactg gttcgacatc aaccgcgtgc aggtctttct
     3061 gaacggccgg cccgagcagg acctcaactt cacgcggcgg acgacaccca accgcttccg
     3121 cgacgaaacg gtgcgcttcg accactcgat cccgctggag ctaaagggcg acactcacgt
     3181 gatcgtggcc accatcggcg aagggctgac cctcggcccc gtcatgggcc ccaactacgg
     3241 cggcaagctc ccgcccgtcg
//
LOCUS       DASUGB010000249         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1278417, whole genome shotgun sequence.
ACCESSION   DASUGB010000249 DASUGB010000000
VERSION     DASUGB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278417"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFV87_04820"
     CDS             complement(<1..424)
                     /locus_tag="VFV87_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5103107.1"
                     /translation="MLLANAESLPAEPVSVIVVGAGPAGLVQALELRRRGLDVVVLAG
                     GVDRLDPEYQALSEIEIADPRRHAPMRLAVARALGGTSLLWGGRCVPFDDIDFRTRSH
                     VPNSGWPLRHSEIRPWYAVAASYLDAGTPIFAAAAEGMP"
     gene            complement(608..1849)
                     /locus_tag="VFV87_04825"
     CDS             complement(608..1849)
                     /locus_tag="VFV87_04825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5103108.1"
                     /translation="MTQLARRHHLTAAMLVDEEFDLEECRQAMQAYCREVVLVPNPYG
                     RHGLAKRLLQLKSLGVAQSFERLRVTVPAFQQTLDRMLRTRRFDVINLEFPYLGHYKL
                     RPAPSRERPPALVIDSHEMAYDLPRQFARAAASPGRRLYGEVDWRKLRREELRTYRDA
                     DGVYLCSSLDERRLLDQLPGVRTAVIPNAADVDFYQPRADDPVQDGRTVVYFGLLSTL
                     PNTDAVGHFAQSIWPEVRAAYPEARWKIIGDRPPLSVQALAGSGIELTGLVPDLRPHL
                     AEAAVVIVPLRLGGGTRLKIVEAMAMGKAIVSTSLGAEGINAVPGRDILIADDPGDFA
                     AAVLKLLNDPSLRSRLGASARQLAVEHYSWIAAAANLEAFFHSLILSQTADACRSRSL
                     DADPSLRVSTSSYRPAPHASS"
     gene            complement(1975..>3337)
                     /locus_tag="VFV87_04830"
     CDS             complement(1975..>3337)
                     /locus_tag="VFV87_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5103109.1"
                     /translation="SVWNGFVVGPGTAVKGVELLWPGRLLEMDGAGKLVAQKDFWRIP
                     DHAPDPTVSEAELAEVLERGLRLHLASDVPLAVFLSGGVDSSAVANLAQRATDSPIHT
                     FTLAFEEQQLNEGPIARQIAAAIGSEHHEVVLTEGDFVESLDAALDSLDQPSFDGLNA
                     YSMSRAIRDAGFTVALSGTGGDELFGGYPSYRDLPMLHRWSRRAAWMPRSHRVAAASL
                     ATWNLRRSGGAVSPQTRWAKLPDMVRHGDDLLALYQLAYSLFLPDFQRDLLAADFAAV
                     LPNGLPTAMRQRVVDETRGRTPLSTISVMEQRLFLGERLLRDNDVASMACSLEQRVPL
                     VDQVLFETVDRLPCQLRYSPLGRKSLLRRVGLRGLDPTMFDRPKSGFVLPFDRWIRGG
                     LKNAMDQTMRDPEAIAPVGLNPVAVERLWRAFLDRAPGMYWSRVWSVYVLIRWCHRNR
                     VFR"
BASE COUNT          646 a         1068 c         1059 g          564 t
ORIGIN      
        1 tcggcatgcc ttccgccgcg gccgcgaaaa tcggggtacc cgcatcgaga tagctcgccg
       61 cgaccgcgta ccacgggcgg atttcggaat gccgcagtgg ccagccgctg ttcggcacat
      121 gcgaccttgt gcgaaaatca atgtcatcga acggtacgca gcgcccgccc cacagcagcg
      181 aagtcccgcc gagggcgcgc gccaccgcga gcctcattgg cgcatgccgt cgtggatcgg
      241 cgatttcgat ttctgataac gcctggtatt cgggatcgag acgatcgacg ccgccggcga
      301 ggacgacgac atcgagccca cgtcggcgca attcaagcgc ctgcaccagc ccggctggtc
      361 cagcgccgac gacgatgacc gaaaccggtt cggcgggaag tgattcggcg ttggcaagaa
      421 gcatccgatg cctttcgatg atatcatcgg cgcactgctc gcgcttaccg cgagcgacag
      481 ttcgccgaat gggcaatgtt atttcgtcga atgaagcgaa gagggcgtcg agagataact
      541 tcggggaccg ttcgcgatgg gcttggcagc tctctccagt tgcttggccg catcgtttct
      601 cgctctgtca tgagctggcg tgaggcgccg gtctgtatga actggtgctg actcgcaacg
      661 atggatccgc atccagcgat ctcgatcggc atgcgtccgc cgtttggctt aagatcaggc
      721 tatgaaaaaa agcttcaagg ttcgccgctg cggcgatcca tgaatagtgt tccaccgcga
      781 gttggcgggc cgaggcgccg agccgggacc gtagtgatgg atcgttcagc aacttcaaga
      841 ccgctgcggc gaaatccccc gggtcatcag cgatcaggat gtcccgccct ggaaccgcgt
      901 tgatcccctc cgccccaagc gacgtggata cgatggcttt ccccatcgcc attgcctcca
      961 cgatctttag gcgggttcca ccgcccagcc gcagcggcac gatcaccacc gcggcttccg
     1021 caaggtgtgg ccgcaagtcc ggcaccaggc ccgtcagttc gatccccgac cctgccaagg
     1081 cctgcaccga aagcggtggg cggtcgccga tgatcttcca gcgcgcctcg ggatacgccg
     1141 cccttacttc gggccagatg gactgggcga agtggccgac ggcgtcggta ttcggcagcg
     1201 tggagaggag gccaaaatac acgacggtgc gcccgtcctg gaccggatca tcggcgcgcg
     1261 gctgatagaa atcgacatct gccgcgttgg gaatgaccgc ggtgcgaaca ccgggaagct
     1321 gatcgagcag acgccgctca tccagcgagc tgcacaggta gacgccatcc gcatcgcgat
     1381 aggtccgcag ttcctctcgc cggagcttgc gccagtccac ctcgccatag aggcgccggc
     1441 ccgggcttgc tgcggcgcgg gcaaactgcc gaggcagatc gtaggccatc tcatgcgaat
     1501 caataaccag cgccggcggt cgctcgcggg acggcgccgg acgcaatttg tagtggccca
     1561 gataggggaa ttcgagattg atgacgtcga aacgccgggt gcggagcatc ctgtcgagtg
     1621 tttgttgaaa tgcaggcacc gtcacgcgaa gccgctcgaa gctctgcgct acgcccaggg
     1681 atttcagctg cagcagacgt tttgcgagcc cgtgccgacc gtatggatta ggcaccagca
     1741 ccacttcgcg gcaataggcc tgcattgcct ggcggcactc ctcgaggtcg aactcctcat
     1801 ccaccagcat cgcagcggtc aggtggtggc ggcgcgccag ttgagtcatc agtccatgaa
     1861 ttcgcgcctg tgcccctgat cgcggcgggc tggcgggcat atgggacacg aggagaatgc
     1921 ggagtcttcc ggaactcatg cgtgacgact ttccaatgtg gctgcgatgt ggcttcagcg
     1981 gaaaacgcga ttgcggtggc accaccggat caacacgtag accgaccaga ctctggacca
     2041 gtacatgccg ggcgctctgt cgaggaacgc ccgccagagc cgttcaacgg cgacgggatt
     2101 gaggcccact ggggcaatcg cttctgggtc tcgcattgtt tgatccatgg cgttcttcag
     2161 accgccgcgg atccagcgat cgaacggcag aacgaacccg ctcttcggtc tatcaaacat
     2221 agtcggatcg aggccgcgca gcccgacgcg ccgcaacaag gatttacgac ccaagggcga
     2281 atagcgtagc tgacacggca atcgatcgac agtttcgaag aggacctgat ccaccagcgg
     2341 cacacgctgt tccagcgagc aggccatgct ggcaacgtcg ttatcgcgca acaggcgctc
     2401 acccaggaaa aggcgctgtt ccatgacact aatggtagag agcggcgtgc gcccgcgagt
     2461 ctcgtcgacg acccgctgcc gcattgctgt cggcaaccca ttgggcagca ccgcggcaaa
     2521 atcggccgcc agaaggtcgc gctggaagtc gggcagaaag agcgaatacg ccaactggta
     2581 gagggccagc agatcgtccc cgtgtcgcac catgtcgggg agcttcgccc aacgggtctg
     2641 aggcgacact gccccgccgc tgcgccgcag attccaggtc gccagcgacg ccgcagcaac
     2701 ccgatggctg cggggcatcc aggcggcgcg ccgcgaccag cgatgcagca ttggcaggtc
     2761 gcggtaggag gggtatccgc cgaagagctc gtcgccacca gtgccggaca gggcaaccgt
     2821 gaacccagcg tcgcggatag cgcgcgacat ggagtaggcg ttgagtccat cgaaactcgg
     2881 ctggtccaga ctatccagcg ctgcatccag gctctcgacg aagtccccct ccgtcagcac
     2941 cacctcgtgg tgctcggacc cgatcgcggc ggcaatctgc cgcgcaatgg ggccttcgtt
     3001 cagttgctgc tcctcgaagg ccagggtgaa ggtgtgaatt gggctgtccg tagcgcgctg
     3061 ggccaggttc gccacagctg acgagtccac gccgccggaa aggaacaccg ccagcggcac
     3121 atcgcttgcg aggtggagcc gcaatccccg ctccaggacc tccgccagtt cggcctcgct
     3181 gacggtcgga tcaggcgcgt ggtcggggat gcgccagaaa tccttctggg caaccagctt
     3241 gcctgcaccg tccatttcca gcagccggcc gggccacagc aactcgacgc ctttgacggc
     3301 ggtgccgggg ccgaccacga acccgttcca aacgctg
//
LOCUS       DASUGB010000250         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_722844, whole genome shotgun sequence.
ACCESSION   DASUGB010000250 DASUGB010000000
VERSION     DASUGB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722844"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFV87_04835"
     CDS             <1..518
                     /locus_tag="VFV87_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103110.1"
                     /translation="RPESAGEAVTMPLSPRDTGSQDEEALQHYMDRFLERVTGKKKSD
                     GETTPDPAVTVVAASAPQEPEKPREIKRPPDSVQDLSQMRALANQSAREALGIHTGRQ
                     IATSARTAGLVALFLAGFSSILAASHLAMTASWMWLGSLAIVPLTLFMTWRFFRLRGQ
                     LLRLEREEATA"
     gene            768..1586
                     /locus_tag="VFV87_04840"
     CDS             768..1586
                     /locus_tag="VFV87_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103111.1"
                     /translation="MKRSAFTVALGLLLGSAAILTAEQPLQSPPEELPIAPVLGGDPI
                     AEAAHAADVWGPPPLPPLPYGFTWDDYPGAKVAHDPYGYQRLSRPPRKTACCYGWLPA
                     LCDGVTHLFAYPTPGSQPHGHGHHCHACAACAAASQCPCGSQCPCGSQHAVMPVDGSY
                     NVPVYQAPAYSAPVYRAPVLDPAYVEVPQPMPDPADEVRVPPPVLIDDGLPAPPVVEA
                     GDPEPPTLVPATPVIIPTAPPRTAPPRNVIPQPVSKTPKSSTSRTFSKMPRGRA"
     gene            complement(1603..>2371)
                     /locus_tag="VFV87_04845"
     CDS             complement(1603..>2371)
                     /locus_tag="VFV87_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103112.1"
                     /translation="SDAMAVIVTQQPPSTRSGVALAVEDDRWSLTLGGMFDDAPPASE
                     AGFREFARGLSSPLIHDFLRSAEPVSEIQVFKYPASLRRRYERLKQFPEGLLVLGDAL
                     CSFNPIYGQGMSTAALEAVLLGECLQHSEPRLAQRFFRGAARIVDVPWQTAATSDLQF
                     PQVRGKRPLLSGLANAYQSLVHRAAQADPEVALAFHRVANLLERPASLLRPRILRRVL
                     HHAWRASEPAECRLVESPTQPPRLICAGIRAGGLRIS"
BASE COUNT          377 a          822 c          776 g          396 t
ORIGIN      
        1 cgcggccgga gtccgccggg gaggcagtta ccatgccgct ctcgccgcgc gacaccggga
       61 gccaggacga agaggccctc cagcactaca tggaccgctt cctggagcgg gtgaccggaa
      121 agaagaaatc cgacggcgaa acgacgcccg accccgccgt caccgtcgtt gctgcctccg
      181 ctcctcaaga gccggaaaag ccgcgggaga tcaagcgtcc gccggactcc gttcaggatc
      241 tgtcgcaaat gcgggccctg gcgaatcaaa gcgcccgcga ggcgctgggc atccacacag
      301 gtcggcaaat cgccacgtcc gccagaacgg ccggattggt cgccctgttc ctggcggggt
      361 tttcgtccat acttgccgct tcgcacctgg cgatgactgc ctcttggatg tggctcggca
      421 gcctcgcgat tgtgcccctt acgctgttca tgacctggcg atttttccgg ttgcgaggtc
      481 aacttctccg gctggaacgc gaggaagcga ccgcctgaag ggccgtttgt gccgctgata
      541 actctggctc acgccgtcag tcctataggg ctggccgcag ttgtgaccgt cgcatggaac
      601 gcaccagcaa gtcgcaatgg tctgtgcaag cggaatgtct tggccccctt ggccagcctg
      661 taggccccct cgcacgcttg acgatagcat cacccgtcgg atgcttgcgg cggattccgc
      721 aagctgcggt cctgacggat ggggacctct agccaggtga ttcacggatg aagcgctccg
      781 catttaccgt cgccctcgga ctcttgctcg gctcggcggc gatcctcacg gctgagcagc
      841 cgctccagag cccgccggag gagctcccga ttgccccggt cctgggcggc gacccgatcg
      901 ccgaagccgc tcatgccgcg gacgtctggg ggccgccgcc gctgcccccg ctcccctacg
      961 gctttacctg ggacgactac ccgggcgcga aagtcgccca cgatccctac ggctatcagc
     1021 gcctgagccg gccgccccga aaaacggcct gctgctacgg ctggttgccg gccttgtgcg
     1081 acggcgtgac gcacctgttc gcctatccca cgcccgggtc gcagccgcac ggtcacggcc
     1141 accattgtca cgcctgtgcg gcatgtgcag cggcaagcca gtgtccctgt gggtcccagt
     1201 gtccctgtgg gtcgcagcat gcggtcatgc cggtggacgg gtcgtacaac gttcccgtct
     1261 atcaggcgcc cgcttacagt gctccggttt accgggcgcc tgtcctggat cccgcctatg
     1321 tcgaggtccc gcagccgatg cccgatcctg cggacgaagt ccgtgttccg ccgccggtat
     1381 tgattgacga cggcttgccg gcgccacccg ttgtcgaggc cggtgatccc gagccgccga
     1441 cgctcgtccc ggcgacgccc gtgatcatcc ccaccgcgcc accgcgaacc gcgccgccgc
     1501 ggaacgtcat cccgcagccg gtctcaaaga ctcccaagag cagcacgtcg cggacgttca
     1561 gcaagatgcc gcgcggtcgg gcttgattgc atttccggtg ctttagctta tgcgtagccc
     1621 ccccgcccgg attcccgcgc aaatcagccg cggcggctgg gtgggggatt cgacgaggcg
     1681 gcactcggcc ggctcgctgg cgcgccaggc gtgatgaagc acgcggcgga gaatcctcgg
     1741 ccgcagcagg ctggcgggcc gctccagcag gttggcgacg cggtgaaacg cgagcgccac
     1801 ttcgggatcg gcttgggcgg cgcggtggac gagcgattga taggcgttcg cgagtcccga
     1861 gagtagcggc cgcttgccgc ggacttgcgg aaactgcaag tcggacgtcg cggccgtctg
     1921 ccagggcaca tcgacgatcc gggccgctcc gcggaaaaac cgctgcgcca gccgcggttc
     1981 gctgtgctgc aagcactcgc ccagcagcac ggcttccagg gcggcggtgc tcatcccctg
     2041 gccgtaaatc ggattgaagc tgcacaaggc gtcgcccagc accaagagcc cctccgggaa
     2101 ttgcttcagg cgctcgtaac gccggcggag actggcggga tacttgaaga cctgaatctc
     2161 ggataccggc tcggccgatc gcaggaagtc gtgtatcagc ggcgaggaca acccgcgggc
     2221 gaactcgcga aagccggctt cgctcgctgg cggagcgtcg tcgaacattc cgcccagcgt
     2281 gagcgaccag cggtcgtctt caacggccag ggcgacgccg ctgcgggtgc tcggcggctg
     2341 ctgcgtcacg atgaccgcca tcgcgtccga g
//
LOCUS       DASUGB010000251         7556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_584228, whole genome shotgun sequence.
ACCESSION   DASUGB010000251 DASUGB010000000
VERSION     DASUGB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584228"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFV87_04850"
     CDS             <1..243
                     /locus_tag="VFV87_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103113.1"
                     /translation="DSPPLSLSLSLALAPTLPCQAGPARFDITALLAPRNELTLDVLL
                     PDIAPGTPPLVRPGREHLPGGPIGLVQLQIEESAGI"
     gene            374..892
                     /locus_tag="VFV87_04855"
     CDS             374..892
                     /locus_tag="VFV87_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103114.1"
                     /translation="MNAIARAPDLQARFGEGGGSSASISILFGPALAVGDSVLDSVSA
                     PALVLQLAPGQIAVIGRAHGREVPYLDPAYRPTSLVPGTGETILRGDGDRRDLTVSRG
                     HFMLSAHPAGLLLTNGVPRRGGGVRPPMNGTLLLGPIQRSMAPGEEQKVPYGSAIVLQ
                     LPNGTQVRIEAR"
     gene            945..1709
                     /locus_tag="VFV87_04860"
     CDS             945..1709
                     /locus_tag="VFV87_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX5103115.1"
                     /translation="MSAVHDWSLVDDGTFHDFHNGWIIYLRDALNRGVLPKGNYAQSE
                     QHSMEYIADVLTLHVPGDPPPDTGERGGVSVLDAPPKVGRRVQMQPPYDKLRKTLAIR
                     RTTGHRVVALIEILSPGNKSGRRKVSEFVRNATDAVRHGCHLLLVDILPPTTFAPRGM
                     HAAIWESWYEKKYALPKNKPLTLCSFESRRHEPVEAWIEHLAVGDTLIDMPLFLREDF
                     YVNIPIQATYDQSFLGVPEVFRAVLEKGMNGKRRKR"
     gene            complement(1737..2321)
                     /locus_tag="VFV87_04865"
     CDS             complement(1737..2321)
                     /locus_tag="VFV87_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103116.1"
                     /translation="MADARRKIWNSWQRAYLVGLAFSPVYGAALAGVGLAIYRTVTGR
                     FGFPAQPGHWVLLAIGGVMGIIASRPVLRTLPLSADGEEFILVCLMLVVATATAIGVG
                     NNVWRLPMACAALGLTIICLSYAISLRSQSIEPPASFIFGFFVIAQVPFLGLLALACD
                     LAGHKRHDIFHWFGVATFVGVLGHLLALVGAGRI"
     gene            complement(2617..3054)
                     /locus_tag="VFV87_04870"
     CDS             complement(2617..3054)
                     /locus_tag="VFV87_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5103117.1"
                     /translation="MRFIDTNVLLYRVSEDPTEREKSEIAGKLLQATDVALSVQVLQE
                     FYVQATRATRANRLSHDEAQALIEAWLRMSVQEMTVAVLRAAVESSKRFRLSYWDAAI
                     IESARALGCKEVLSEDLSAGQSYAGVRAVNPFAGKKPKSNGKK"
     gene            complement(3060..3305)
                     /locus_tag="VFV87_04875"
     CDS             complement(3060..3305)
                     /locus_tag="VFV87_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103118.1"
                     /translation="MKNITVSLDDEVYRRARIKAAELDTSVSALVRKYLVELAGQETD
                     FERRKRLEVEVLAKIKGRGFSAKDRLSRDEVHERCVR"
     gene            3430..4383
                     /locus_tag="VFV87_04880"
     CDS             3430..4383
                     /locus_tag="VFV87_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103119.1"
                     /translation="MSMQSLRWRGLLGVLVGWIALVSQALAADTAILGKLPEDFVVYA
                     VGTYGGTRMIDAQIDQSGHEAGQVDVVVNVPDRPVLLVLTAYDPVVWRVGSTSESKIV
                     GVIASGYHTQLVGGIDKDTPRATSTYESPGKFDYFYASGASPELLEMNERVKKLTGKE
                     IERFIHKPQGPAQERVFYVGEPPKDTAKLVYQDEVDLTPFKDPKRPAAGQRGIDQLIA
                     AGKLRRATQEDIDGWVEKASEKYKRLSPDLKVDHHMRAFRTYVVLKPLDLPDGMYGAH
                     SAAFIVPQGVEMPTGPAGHNEFYLMDGTKQDAFTRAFNRGR"
     gene            4605..5216
                     /locus_tag="VFV87_04885"
     CDS             4605..5216
                     /locus_tag="VFV87_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103120.1"
                     /translation="MRRRLDALKQQGARDRQRKDLIWYLLLSAATTLGNASGFDRLFG
                     NHRLTATVSYAALVRLKPAQQRKRLLAALKQAGVRYASKKSKQLSANLREIQRRGGVE
                     SETRRALSLQGRDSKRNYFLQFAGIGPKYANNIWMDIYDDDFHDCVAVDARLSRVLHE
                     LQVDVTCSQSFFQQIARDANLQTWEVDRLLFGFTDYFIAAIRW"
     gene            complement(5261..6745)
                     /locus_tag="VFV87_04890"
     CDS             complement(5261..6745)
                     /locus_tag="VFV87_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5103121.1"
                     /translation="MATATEVAAPKTTPRPRIHQTQCFIDGQWVPAQSGKTFETLNPA
                     TEEVIASVAEGDAADIDLAVKAARKQFDGGEWSKLDARDRGRLINKLADLIEEEAADL
                     AALETLDNGKPIRDSKAADLPLTIDALRYYAGYADKIHGQTIPVRGNYFTYTRKEPVG
                     VVGQIIPWNFPMLMVAWKWGPALAAGCTIVMKPAEQTPLTCLRMARLAQKAGIPDGVI
                     NVVPGYGPTAGAAMVKHPGIDKIAFTGSGETAQIIMRDAAPTLKRITFELGGKSPNII
                     LPDADIDAAIAGAHFGLYFNQGQCCCAGSRLFVQEKIYDQFIERLSSINATRKLGDPL
                     DPATEQGPQVDRAQFDKVMHYIALGNKEGAKCVTGGKRHGTKGYYIEPTLFVDVKDEM
                     AIATDEIFGPVMSVLKFGSIDEIAARANNTTYGLAAAVWTRDIAKAHALAAKLRAGTV
                     WVNCYDVFDAAAPFGGYKMSGMGRELGEEGLKAYTETKTVTVAL"
     gene            7029..>7556
                     /locus_tag="VFV87_04895"
     CDS             7029..>7556
                     /locus_tag="VFV87_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEX5103122.1"
                     /translation="MAGILAIALVSGRSIADESAVASHYGEHIRPLLARHCFACHAIE
                     EPEGDLRLDKLSLDFADNPTRDAWVKVLSRLKSGEMPPKEKPRVPEQELAALVEWVRT
                     NVEPAEAAARAARGRTVLRRLNRAEYQNTIRDLLGVDVDLTQLLPEDTAAAGFDNVGE
                     GLHTSSFLMERYLDAA"
BASE COUNT         1420 a         2346 c         2356 g         1434 t
ORIGIN      
        1 gactctcccc cgctctccct ctccctctcc ctcgccctcg ctcccacgct tccctgccag
       61 gctggccccg cccgcttcga tatcaccgcc ctgctcgccc cgcgaaatga gctgacgctg
      121 gacgtgcttc tgcctgacat tgcaccggga actccgcctc tggtgcgccc cggccgcgag
      181 catctccccg gcgggcccat tggcctggtg cagcttcaga tcgaggaatc cgccgggatt
      241 tagaactcag ccacttttcg tcttgtagca gcgagcccac gattctttcc gccctgaaaa
      301 gctctgcggc gtataatcct ccacgcggtt ttcggcctcg caggtgcccc tccttctgct
      361 gctggagcgg ccaatgaacg ccattgcacg ggcccccgac cttcaagcca ggttcggcga
      421 gggcggtggc tcatccgcca gcatctcgat tctctttggc ccggccctgg cggttggcga
      481 ttcggttctc gattccgtct ccgcgcccgc tcttgtgctg cagctcgcgc caggccaaat
      541 cgcggtcatt ggccgagccc acggccgcga ggtgccgtat ctcgacccgg cttaccggcc
      601 gacttcgctg gttccgggca ccggcgagac gatcctgcgc ggcgatggcg accgccgcga
      661 tctgacggtc agccgcgggc atttcatgct gtcggctcat cccgccggcc tgctcttgac
      721 caacggcgtg ccccgccgcg gcggcggcgt ccgcccaccc atgaacggca cgctgctctt
      781 gggtcccatc cagcgctcga tggctcccgg cgaagagcaa aaggtcccct acggctccgc
      841 catcgtgctg caacttccca acggcacgca ggtgcggatt gaggcgcggt agtgcgactc
      901 tgctcgtgtt agaattgaga atcgcacgag ggagggtcgc attgatgtcg gcagtgcatg
      961 attggtcgct cgtggacgac gggacgttcc acgacttcca caacggctgg attatctatc
     1021 tgagggacgc tctgaatcga ggcgtgctgc cgaagggcaa ctatgcgcag tccgagcaac
     1081 actccatgga gtacatcgcc gacgtgctga cgctgcacgt ccctggtgac ccgcccccag
     1141 acaccggcga aagaggcgga gtaagcgtgc tcgatgcgcc gccgaaggtc ggccgcagag
     1201 tgcagatgca gccgccttat gacaagctgc gcaagactct cgccatccgc cggacaacgg
     1261 gacaccgggt cgtggctttg atcgaaatcc tctcgcccgg caacaagagc gggcggcgca
     1321 aagtcagcga gttcgtccgc aacgcaaccg acgccgtccg ccacggctgt cacctgctat
     1381 tggtcgatat cctgcctccg accacgttcg cgccacgcgg catgcacgca gccatttggg
     1441 aatcgtggta cgagaagaag tacgcgctgc ccaagaacaa gccactgaca ctctgctcgt
     1501 tcgagtcgcg ccgccacgag cccgtcgaag cgtggatcga gcacctggcc gtcggcgaca
     1561 ccctgatcga catgccgctc ttcctgcgcg aggacttcta cgtcaacatt ccgatccagg
     1621 cgacctacga ccagtcgttc ctcggcgtgc cggaggtctt tcgcgccgtg ctggagaagg
     1681 ggatgaacgg caagcggcgc aagcggtagt tttcggcacc ctattctatg ccgtactcag
     1741 attcggcctg cgccaaccaa cgccagcaaa tgccccagca cgcccacaaa cgtggccacg
     1801 ccaaaccagt gaaagatgtc atggcgtttg tggccggcca gatcgcatgc cagggcgagc
     1861 agccccagga agggcacctg cgcgatcacg aaaaaaccga aaatgaaaga cgctggcggt
     1921 tcgatggatt gggatcgcaa cgaaatggcg taggacaggc agatgatcgt cagtccaagc
     1981 gcggcgcaag ccatgggcaa gcgccacacg ttgttgccga ccccgatggc cgttgcagtt
     2041 gcgaccacga gcatgagaca taccaagatg aattcctcgc catcggcgga gagaggcagc
     2101 gtgcgcagga caggacgcga agcgatgatc cccatcactc cgccgatcgc cagcaatacc
     2161 caatggcctg gttgggccgg aaatccgaac cgtcccgtga ccgtcctgta gatagcgagg
     2221 cccacgccgg ccagcgccgc gccgtaaacg ggcgaaaacg ccagtccgac gaggtaggcc
     2281 ctctgccaac tgttccagat cttccgtcgg gcgtcggcca ttgccgcttt cacgtccgta
     2341 ccgggctgtg acaaaaagct tgcaaatcca atcgactcgg gtggcggagg tcgccgcgac
     2401 tgaaagtaca aaagagccag gcccgtcgcc gcggtccaca gcagcaaatg cgcgatcgtc
     2461 agccgattgc ggcgcggcag cacgttgcca agcgcagtcg attcagcccg tagctggctc
     2521 acacgtgcct cgcaggcgac cggttgcgaa tcgaggcctg agaagggatt cgcggcttgc
     2581 gccggacatt ctaccgccgt agagggcgac accaagctat ttcttcccgt tcgacttggg
     2641 tttcttgccg gcgaacggat tcacggcccg cacgccggca tagctttgtc cggcgctgag
     2701 gtcttcggac agcacttcct tgcatcccag cgcgcgggcc gactcgatga tcgcggcgtc
     2761 ccagtaggac aagcgaaatc gcttgctgga ctcgaccgcc gcgcgcagga cggcgacggt
     2821 catttcctgc accgacatgc gtagccatgc ctcgatcagc gcttgagcct cgtcatgcga
     2881 caaacgattg gctctcgtgg ctcgggtcgc ctgaacgtag aactcctgca gcacctggac
     2941 cgacaaggca acgtcggtgg cttgcaagag cttcccggcg atctcggact tttcacgctc
     3001 cgtgggatcc tcgctgaccc ggtacagcag aacattggtg tcgatgaacc gcacgcgact
     3061 cacctcacgc agcgctcatg gacttcgtca cgactcaggc gatccttcgc tgaaaagcca
     3121 cgacctttga tctttgccaa cacctccacc tccaatcgtt tccgtcgctc gaagtccgtt
     3181 tcctggccgg ccagctcgac gaggtacttc ctgaccaaag ccgagaccga agtatccagc
     3241 tccgcggcct tgatccgcgc ccggcgatag acctcgtcgt ccagtgaaac tgtgatgttc
     3301 ttcatagttt cacagtaccc ttttccgggc gggccgtcca gtcggacttg cgacgctgat
     3361 tgacagaccc agccaagaag cggcgataat gggcagggcc gacagaagaa caatcctgga
     3421 ggtatggcaa tgtcgatgca atcgcttcgc tggcggggac tcctcggtgt gctcgttggt
     3481 tggatcgcac ttgtctcgca ggcactggcc gcagacacgg cgattctggg aaagctgcct
     3541 gaagacttcg tcgtttacgc ggtggggacg tatggcggta cgcgtatgat cgatgcccag
     3601 atcgaccaaa gtgggcacga agccgggcaa gtcgacgtcg tggtgaatgt cccggatcgc
     3661 cctgtcctgt tggtgcttac tgcctacgat ccagtcgtgt ggcgcgtggg tagtacgtcg
     3721 gaatcgaaaa tcgtcggcgt cattgccagc ggctatcaca cgcagctcgt cggcggaatc
     3781 gacaaggaca cgccgcgtgc cacctccacc tatgaaagcc ccggcaaatt tgactacttt
     3841 tatgcgagcg gggcatcgcc ggaattgctc gaaatgaatg aacgcgtgaa gaagctgacc
     3901 ggcaaagaga tcgagcgttt cattcacaag ccgcaagggc cggcgcagga gcgtgtgttc
     3961 tatgttggcg agccgcccaa ggataccgca aagctggtgt atcaggacga ggttgatttg
     4021 acgccgttca aggatccgaa gcgccctgcc gcgggccagc gagggatcga tcaactgatc
     4081 gcagcgggca aactaaggcg tgccactcaa gaggacatcg acggctgggt tgaaaaggcg
     4141 agcgagaagt acaaacgatt gagtccggac ctaaaggtgg atcaccacat gcgggccttt
     4201 cgcacctatg tggtcctcaa acccctggat ctgcccgacg gaatgtatgg cgctcattcc
     4261 gcggcattca tcgtgccgca aggagtcgaa atgccaaccg ggccagcggg ccacaatgag
     4321 ttctacctga tggacggcac gaagcaagat gcgtttactc gagcgttcaa tcgaggacgc
     4381 taggcgccgg aatctctcta cattgccgcg ttcgcgctgc ggccgcaact tcccaacggc
     4441 acgcaggtcc tgcttgcgtc ggcaatgctg gaactgattt aggagggcct agaacggggc
     4501 cgcggagcag ctagagcggg gtgaggggcg gaaatgtaat ggatttgccg tcacaccagc
     4561 gacgcgagaa actcgtcgaa gcactccgct gcctcactcc ggtgatgcgc cggcggctcg
     4621 acgccctcaa gcagcagggt gctcgagacc gtcagcgcaa agacttgatc tggtatctcc
     4681 tcttgtcggc ggcaacgacc cttggcaatg catccgggtt tgatcgcctg tttggcaatc
     4741 atcgattgac cgcgactgtt tcctacgcgg cactcgttcg actcaagccc gctcagcaac
     4801 ggaagcggct cctggccgcg ctgaaacagg caggagtacg gtacgcgagc aagaagtcaa
     4861 agcaactttc agccaatttg cgagagattc aacggcgtgg cggcgtcgag tcggaaacga
     4921 ggcgagcgct gtcgctgcaa ggacgcgatt ccaagcgcaa ttactttctg cagtttgcag
     4981 gcattggtcc caaatacgcg aacaatattt ggatggacat ttacgacgac gactttcacg
     5041 actgcgtcgc cgtcgatgcg agactgtcgc gcgtcctgca tgagctacag gtagacgtca
     5101 cttgcagcca gagcttcttt caacagattg cccgcgacgc aaatctgcag acgtgggagg
     5161 tagaccggtt gctctttgga ttcactgact acttcattgc agcgattcgg tggtgactat
     5221 gaacgcggct tggcgggcag gcgggacgac taccccaccg ttacagggcg accgtgaccg
     5281 tcttcgtctc ggtgtaggcc ttgagcccct cttcgcccag ttcgcggccc atgccggaca
     5341 tcttgtagcc gccgaagggg gcggcggcgt cgaagacgtc gtagcagttg acccagacgg
     5401 tgccggcccg cagcttggcg gccagggcgt gggccttggc gatgtcgcgc gtccagacgg
     5461 cggcggccag gccgtaggtg gtgttgttgg cgcgggcggc gatttcatcg atcgatccga
     5521 acttcagcac tgacatcacg gggccaaaga tctcgtcggt ggcgatcgcc atctcgtcct
     5581 tcacatcgac gaacagcgtc ggctcaatgt agtagccctt ggtgccgtgc cgcttgccgc
     5641 cggtgacgca cttggcccct tctttgttgc ccagggcgat gtagtgcatc actttgtcga
     5701 actgggccct atcgacctgc gggccctgct cggttgccgg gtcgagcggg tcgccgagct
     5761 tccgcgtggc gttgatcgac gacagccgct cgatgaactg gtcgtaaatc ttctcctgca
     5821 caaacagccg gctgccggcg cagcagcact ggccctgatt aaagtagagg ccgaagtggg
     5881 cgccggcgat cgcggcgtcg atgtcggcgt cgggcaggat gatgttcggg ctcttcccgc
     5941 ccagctcgaa cgtgattcgc ttcagcgtcg gcgcggcgtc gcgcatgatg atttgggccg
     6001 tttcgccgct gccggtgaag gcgatcttgt cgatgcccgg atgcttgacc atcgccgctc
     6061 cggcggtcgg gccgtagccc ggcacgacgt taatcacgcc gtcgggaatg cccgctttct
     6121 gggcgaggcg ggccatccgc aggcaggtca gcggcgtctg ctcggccggc ttcatgacga
     6181 tcgtgcagcc ggcggccagg gcggggcccc atttccaggc gaccatcagc atcgggaaat
     6241 tccaggggat gatctgcccc acgaccccga ccggctcttt ccgcgtatag gtgaagtagt
     6301 tgccgcggac ggggatcgtc tgcccgtgga ttttgtcggc gtagccggcg tagtagcgca
     6361 aggcatcgat cgtcagcggc aggtcggcgg ctttgctgtc gcgaatcggc ttgccgttgt
     6421 cgagcgtttc gagggcggcc agatcggcgg cctcctcctc gatcaggtcg gccagcttgt
     6481 tgatcaggcg gccgcggtcg cgggcatcca gcttgctcca ctcgccgccg tcgaactgct
     6541 tgcgggccgc cttcacggcg aggtcgatat cggccgcgtc gccttccgcg acgctggcga
     6601 tgacttcctc agtggccggg ttgagcgttt caaacgtctt gccgctttgc gcgggaaccc
     6661 attggccgtc gatgaagcac tgggtttgat gaatgcgggg gcggggcgtg gttttgggag
     6721 cagcaacttc ggtagccgta gccatggcga tactcctgag agagcggagt tggaattgac
     6781 gggcaaatcg gacctccagg tctgatgtac gaattgtacc gatttaacgc cgcgggcggc
     6841 aatgcaaagt aaatgcccag agcgacatcg agcgatggga caattcgcac gccttcgctt
     6901 gtaacgcgtg gccaatctgc tgacaatcta tcaagacggc aaaatgagcg gccggccatc
     6961 agggatggca gccgcaggca ggcggaggtt ccacgttggt cacagcacac cgatttctga
     7021 gcgcctgcgt ggcaggcatt ttggcgatcg cacttgtgag cgggcgaagc atcgcggatg
     7081 aatcggctgt cgccagtcat tacggcgagc acatcaggcc gctcttggcc cgccactgct
     7141 ttgcttgtca cgcgatcgaa gaaccggaag gggatttgcg gctcgataag ctctcgttgg
     7201 attttgcaga caaccccacc cgtgatgcct gggtcaaggt tctttcgcgg ctcaagagcg
     7261 gcgagatgcc gcccaaggag aagccgcgag tgccggagca ggagcttgcg gcgctggtgg
     7321 agtgggttcg cacgaatgtc gagccagcag aagcggctgc gcgggcggcc cggggccgca
     7381 cggtgctgcg tcggctgaac cgggcggagt accagaacac aattcgcgat ttgctcggag
     7441 ttgatgtcga tctgacgcag cttctgccgg aggacactgc ggctgcgggc ttcgacaacg
     7501 tcggcgaggg attgcacacg tcgtcgttcc tgatggagcg ctacctcgac gccgcc
//
LOCUS       DASUGB010000252         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1974628, whole genome shotgun sequence.
ACCESSION   DASUGB010000252 DASUGB010000000
VERSION     DASUGB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974628"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..>2097)
                     /locus_tag="VFV87_04900"
     CDS             complement(327..>2097)
                     /locus_tag="VFV87_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103123.1"
                     /translation="KPQTFLGFATKMGESMDSDHVATLCLAHWPGQASPWMDDLRRIA
                     RYCSALGKLVTVDEYFASTDQPGQLDRFEADRYRSPYLKQAVIRKHDDPISTQVRYWQ
                     RQVAAGAAEAMLALAALVEGKGPGDREQESGVRGQELDLASGGREPPESSAPAVDRGL
                     AAQLDAAKARLAAAVSASNPNGPKGPLILNPLSFIRRIGVEGLDLAGLPTIERPVYAA
                     DEGAGGAQAVVDAPAFGFVHLTPGKSAQRDKKTLLLAEENLLRNEFFEAIINPTTGSL
                     AAIHEYKARGNRLSQQLALRLPGPKQKPGDTYRDPDESAVYSVMAADSVETTIATTAL
                     GEIVARGRLLDQHGSKLAGFVQTYRLWRGSRVLHVEIELDPVEEPKADPWNSYYCVRW
                     AWPDESADLHRTVNETRQAVTEKRFESPHYVEITDEKTSTTILTGGLPFHRRQEERML
                     DTLLITRGERERKFRLGIGVDLSHPLHEALGLFLPPLVVPNVPQPKSGNSGWLLHLSS
                     RNVIVTSLQPLEEGGRIVGFTARLLETAGRPASLAVSAFRPVKSASTVDFAGQPLGDC
                     PIEDGKIKFDLAAHEWTQIAARW"
BASE COUNT          339 a          675 c          708 g          375 t
ORIGIN      
        1 agctccgtcg gactctgggc ccagtggtca caagcgcagc ggatgcgcgg acgtattcac
       61 cggttgaccg gcaaagccgc cttggcattc gcgtttaatc gcctgactga gccacagaac
      121 cgacatgact ggtattctcc ctgcctcacg ggtgctggcc cgtgggatca atggtttaga
      181 cgccgcgggg cggaaaaagg ttcgccgaaa atccgatgcg cttggggcag actttatttc
      241 cagaatcttg ccgctgtggt gcaaaatcat cgctcaaccg agcaagctcg gcgggggcgg
      301 ggatgagcta ccccattttc ccagggctac cagcgggcgg caatttgggt ccactcgtgg
      361 gcggccaggt cgaacttgat cttgccgtcc tcgatcgggc agtcgccgag cggctgaccg
      421 gcgaaatcga ccgtgctggc cgatttcacg gggcgaaacg cgctcacggc cagactcgcc
      481 ggccggccgg cggtttctaa gagccgggcc gtgaagccga cgatccggcc cccctcctcc
      541 agcggctgca ggctggtgac gatgacattg cgggagctga ggtgcaggag ccagccggaa
      601 ttgccggact ttggctgcgg cacgttcggc acgaccagcg gcggcaggaa gaggcccagc
      661 gcctcgtgca gcggatggct cagatcgacg ccgatgccca ggcggaactt ccgctcacgc
      721 tcgccgcgcg tgatgagcag cgtatcgagc atccgctctt cctgccggcg atggaacggc
      781 aagccgccgg tgagaatcgt ggtgctcgtc ttctcgtcgg tgatctcgac gtagtgcggc
      841 gattcgaacc gcttctcagt cactgcctgc cgcgtctcgt tcaccgtgcg gtgcaggtca
      901 gccgattcgt cgggccaggc ccagcgcacg cagtagtacg agttccacgg gtccgctttc
      961 ggctcctcca ccgggtcgag ctcgatctcg acgtgcagca cgcggctgcc gcgccacaag
     1021 cgatacgtct gcacaaagcc ggcgagcttg ctgccgtgct gatcgagcag gcggccacgg
     1081 gcgacaatct cgccgagcgc ggtcgtggcg atcgtcgttt cgaccgagtc ggccgccatc
     1141 accgaataga cggccgattc gtccgggtcg cggtaggtgt cgccgggctt ctgcttgggg
     1201 ccgggcaggc gcagggcgag ctgctgcgaa aggcgattgc cgcgggcctt gtattcatga
     1261 atggcggcca gcgacccggt cgtgggattg atgatcgcct cgaagaactc gttgcggagc
     1321 aggttctctt cggccagcag cagcgtcttc ttatcgcgct gggccgattt gcccggcgtc
     1381 agatgcacga agccgaacgc cggggcatcg acgacggcct gcgcgccgcc cgcgccttcg
     1441 tcggctgcat agacggggcg ctcgatcgtc ggcaggcccg cgagatcgag gccttccact
     1501 ccgatacgtc ggatgaagct cagcggattg aggatcagcg ggcctttggg gccgttggga
     1561 ttcgaagcag aaactgcagc ggcgagtcgg gccttcgcgg cgtcgagttg ggcagccaat
     1621 ccgcgatcga cggcgggtgc ggagctctca gggggctcac gccccccgct cgccaaatct
     1681 aactcctgac ccctgactcc tgactcctgt tccctgtccc ctggcccctt gccttccacc
     1741 agcgccgcca gcgcgagcat cgcctctgcc gcgccggccg ccacctgccg ctgccaatag
     1801 cggacctgcg tcgagatcgg atcgtcgtgc ttgcgaatga ccgcctgctt gagatacggc
     1861 gagcggtagc ggtcggcttc gaagcggtcg agctgccccg gctggtcggt gctggcgaag
     1921 tattcatcga ccgtgacgag cttgcccagg gccgaacaat aacgggcgat ccgccgcagg
     1981 tcgtccatcc aggggctggc ctggccgggc cagtgggcca ggcagagagt ggcgacgtgg
     2041 tccgagtcca tcgactcgcc catctttgtg gcgaagccga gaaaggtctg cggcttg
//
LOCUS       DASUGB010000253         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1279733, whole genome shotgun sequence.
ACCESSION   DASUGB010000253 DASUGB010000000
VERSION     DASUGB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279733"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2452
                     /locus_tag="VFV87_04905"
     CDS             <1..2452
                     /locus_tag="VFV87_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103124.1"
                     /translation="EAVDSLVVYRAAEGVARAISVWHKGGAAAGDVAFWAPHAEMFDS
                     PKAYALVIEALLERGDHVAAMALLIHWLGQAEQIGLERADASFHDLVLRWFSDITSGA
                     GCSSPLSPRGSGVGGEGAKQETTAEGKAAPACDRWDRARKSLDFIEANAEAFWSVPSF
                     HVGKNGGLAAAAGGADLDDPDEDEGRFGAAYEDVVFRGSTDDGIEGSLFEFDETSHDE
                     LAAESKRVAQRLDFLTTVASLWQMTAVSPAFLAVTQLPADSPELAARAAALGRWIEQA
                     EANLSALSRLMTEVREFRIPLPSADHDSNVEYDRRRMTKESLLERIINTSVETANSAR
                     LLRAALAVVQPDGGEDAANDEQRRLVPLFAALLKSDAAAARALALEARQELARHPLLY
                     VPLAKGGEPQEIVAVRVRQQAIQNLLSWLPRLGLWTETCEILEVARTMERDHPVGPGA
                     VTEFDELFKIGYRAIVESLVASSQTWHTSEGGEDRADALVSCLEKLTESLLASWLAHS
                     RTLRLSVLERVHDRTTWKKVVAFIERYGEELFTQRFLNLSNIRAILHQGTAQWLSKLQ
                     EDLQSRDYEGEIPQRLLDDLAAAAPLDDAAEQLSLVLESIVENYGEYRDYNSTTTQSD
                     RGELLYMLLDFLRLRSRYDRVCWNLKPVVLAHEILVRRGEDQAARTWRKALTERINDE
                     ANQYLTKLAELQKKYAMRMPTVADRLGERFVRPLAIDRIRALVAPAISEARAGNPGPK
                     FQTLQHDTEYLTREPTGVGLDVPAWLVALEEEVEDALRPDYQRQHEQEIADILPAVHL
                     TYDQAKTQLDDWANRKQA"
     gene            complement(2394..>2917)
                     /locus_tag="VFV87_04910"
     CDS             complement(2394..>2917)
                     /locus_tag="VFV87_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103125.1"
                     /translation="FPGPDLFSRLRQRPCAPLLTMMWRRLEKFDAARLARRGQRGREL
                     AGRLAPHYETPGSAAAEHSYWVMPVLADDPPATIARLARAGFHATQGRSLCVVPAPAD
                     SPQHLQPIRAKQLLRQAVFLPVYPEVPGDELDRLANVLTMQSALEVEPKRPLATPASG
                     WPNRRVAFWPDRK"
BASE COUNT          511 a          976 c          986 g          444 t
ORIGIN      
        1 cgaggcggtc gattcgctcg ttgtgtaccg tgcggccgaa ggagtcgccc gggcgatcag
       61 cgtctggcac aagggcggtg cagccgcggg cgatgtggct ttctgggcgc cgcacgccga
      121 gatgtttgac tcgccgaagg cgtacgccct ggtcatcgag gccctgctgg agcgcggcga
      181 tcacgtggcg gccatggccc tgctgatcca ctggctcggc caggcggaac agatcggact
      241 ggagcgcgcc gacgcctcgt tccacgacct ggtcctgcgg tggttcagcg atatcacgtc
      301 cggcgcggga tgctcgtctc ccctctcccc tcgtgggagc ggggtcgggg gtgagggggc
      361 aaagcaagaa acaacggccg aaggcaaggc ggctcccgcc tgcgaccgct gggaccgggc
      421 ccgcaaatcg ctcgatttca tcgaagccaa cgccgaagcg ttttggagcg tcccctcgtt
      481 ccacgtcgga aagaacggcg gcctggcggc agccgctggc ggcgcagacc tggacgatcc
      541 cgacgaagac gagggccggt ttggagcggc ctacgaagac gtcgtctttc gcggttcgac
      601 cgacgacgga atcgagggct cgctcttcga gtttgacgaa acctcgcacg acgagctggc
      661 ggcagaatcg aagcgcgtcg cccagcggct ggactttttg acgaccgtcg ccagcctgtg
      721 gcagatgacc gccgtcagcc cggcctttct ggccgtcacg cagctaccgg cagattcgcc
      781 cgagctcgcc gcgcgggccg ccgcgctggg ccgctggatc gagcaggccg aggcgaacct
      841 gagcgccctc tcgcggctga tgacggaggt tcgcgagttt cgcattccgc tgccgtcggc
      901 cgatcacgat tcgaatgtgg agtacgaccg gcggcggatg acgaaggaat cgctgctgga
      961 gcggatcatc aacacgagcg tcgaaacggc caattccgcc cggctactgc gggctgcgct
     1021 ggccgtcgtc caaccggacg gcggggaaga cgcagccaac gacgagcagc gccgcctggt
     1081 accgctgttc gccgcactgc tcaagagcga tgccgcggcg gcccgggcgc tggcgctgga
     1141 agcccgccag gaactggccc ggcatccgct cctctatgtg ccgctggcca aagggggaga
     1201 gccgcaggag atcgtcgccg tccgcgtgcg gcaacaggcg attcagaacc tgctctcctg
     1261 gctgccgcgc ttggggctgt ggaccgagac gtgcgagatc ctggaagtcg cccgcacgat
     1321 ggagcgcgac catcccgttg ggccgggggc cgtcacggaa ttcgacgagt tgttcaagat
     1381 cggctaccgg gcgatcgtcg agagcctggt cgcctcgtcg caaacctggc acaccagcga
     1441 aggaggcgag gaccgcgccg acgccctcgt ttcctgcctg gaaaagctga cggaatcgct
     1501 cctggcaagc tggctggccc acagccgtac gctgcgcctc agcgtgctgg agcgggtcca
     1561 cgaccggacg acgtggaaaa aggtcgttgc tttcatcgag cgctatggcg aggagctatt
     1621 cacccagcgg ttcctgaacc tgagcaacat ccgcgcgatc ctgcaccagg ggacggccca
     1681 gtggctatcc aagctgcagg aggacctgca aagccgcgat tacgaggggg aaatcccgca
     1741 gcgtctgctc gacgatctgg ccgcagccgc gccgctggac gacgcggccg aacagctcag
     1801 cctggtgctg gagtcgatcg tggagaatta cggcgaatat cgcgattaca acagcacgac
     1861 gacgcagtcc gaccgcggcg agttgctcta catgctgctc gatttccttc gcctgcggtc
     1921 gcgctacgat cgcgtctgct ggaacctcaa gccagtggtg ctggcccacg agattctcgt
     1981 ccgccggggc gaagaccagg cggcccgcac ctggcggaag gccctcaccg agcggatcaa
     2041 cgacgaagcc aaccagtacc tgacgaagct ggccgagttg cagaaaaaat acgctatgcg
     2101 gatgccgacg gtcgccgacc gcctgggcga gcggttcgtg cggccgctgg cgatcgaccg
     2161 catccgggcc ctcgtcgccc cggccatttc cgaggcccgc gccggcaatc ccggcccgaa
     2221 gttccagacc ctgcagcacg acaccgagta tctcacccgc gagccgaccg gcgtcggcct
     2281 cgacgtcccc gcctggctcg tcgccctgga ggaagaggtc gaggacgctc tccgtcccga
     2341 ctaccagcgg cagcacgaac aggagatcgc cgacatcctg cccgccgtcc acctcactta
     2401 cgatcaggcc aaaacgcaac tcgacgattg ggccaaccgg aagcaggcgt agcaaggggc
     2461 cgcttcggct caacttccaa cgcactttgc atcgtaagga cgttcgccag ccggtcgagt
     2521 tcgtcgcccg gcacctcggg atagaccggc aagaaaaccg cctgccgcag aagctgcttt
     2581 gctcggatag gctgcaagtg ttgtggcgaa tctgcgggtg ccggcacgac gcacaggctg
     2641 cgtccctgcg tggcgtgaaa acctgcgcgg gccaggcggg cgattgtcgc cggcgggtcg
     2701 tcggccagga ccggcatgac ccagtagctg tgctcggcgg cggcgctgcc gggcgtctcg
     2761 tagtgcggcg ccagtcgccc agcgagttcg cggccgcgct gaccgcggcg ggccagccgc
     2821 gccgcatcaa acttctccag ccgccgccac atcatcgtca agagcggcgc gcaaggccgc
     2881 tgtcgcaatc ggctgaacag gtccggtccc gggaatc
//
LOCUS       DASUGB010000254         6138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1002852, whole genome shotgun sequence.
ACCESSION   DASUGB010000254 DASUGB010000000
VERSION     DASUGB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002852"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1664
                     /locus_tag="VFV87_04915"
     CDS             498..1664
                     /locus_tag="VFV87_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103126.1"
                     /translation="MLAQIIRNDEELSVGTGGPEIVQQSKRTVYEGSAESVEYTVTTR
                     LAGGRKVTTILRVDPQTHLPQSATIRQSEGSELLCRFDYPEFGPEDVYALGVAKNAIV
                     VDRVPSADLQRVATGVLNGRRQFDDYYCLLVHSSANQHWSQASRMYRTWRKGDCWRIE
                     QSFTDPEDDEALARQPLPPDDVDRKAYWFQRAKETRFYPVLVCDGQHIWRFKQEFDQP
                     TEKRFTFKTTGAERDPVDFDQSFVMLPLFTPAPELEGHSAPLGGTSAKVVERLNPHPD
                     QGPAGTVLVEREYIGRRGQPQKQDLDRYWVDPVNGYLVMRHEMVDLDHDPPQIIQSRE
                     IADWKRSPRGFFYPVELKWDESGIAHYYLDFVTPDAAMFSPKATESIKQRVATR"
     gene            1939..2541
                     /locus_tag="VFV87_04920"
     CDS             1939..2541
                     /locus_tag="VFV87_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5103127.1"
                     /translation="MPPPPETRETLIRRLPNAADVEAWEAFVEIYEPLLFRLARGRGM
                     QPADAEDYVQEVLAAVARNIDRWVASETRGPFRAWLFRIAFNLGVNFVTRPMHQRLGS
                     GDSQIARMLEEVPAVAADSSALFLQEYRRELFRWAAERVREQVSPQQWMVYWLTSVEE
                     RPIAEVAKEYGMSLGSVYIARSRITKRIREMIREHEERSQ"
     gene            2538..4085
                     /locus_tag="VFV87_04925"
     CDS             2538..4085
                     /locus_tag="VFV87_04925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103128.1"
                     /translation="MNSSWNPCQDAHLDVLLDGDEDSEQFLRAASHVESCPACQRRLT
                     ELSGDAKYWAVQREMLRPAPGDQPQSGLLCRHRSTVSAWCRRPVVDESSVQELLAAPS
                     HPEMLGRLGRYEIERIIGTGGMGIVLKGFDSELNRPVAIKLLAPHLARVGAARQRFAR
                     EARAAAAVVHEHVVPIHNVESEHAIPFLVMQFIPGESLQARVEREGPLSVAEILRIGR
                     QAAAGLAAAHAQGLVHRDVKPANILLENGVDRAYLTDFGLARASDDASLTYTGVVAGT
                     PHYMSPEQADGQPLDRRSDLFSLGSVLYFMATGHPPFRADRPMAVLKRTYHDPHRPAW
                     QCNAEIPDSLSQIIDRLLQKKPASRFASAGDLEQALAAVLVEVQQGRLGRRPRYRWRR
                     AQWAIVLGVVAVVALGLAIGPFLMQPASNQANPNASVLPAGGQPTDVAGPDHQSAATE
                     LNAVDPSPLSAAQSEFDSLSGMLDALETTPFPETVFGAQESPGENRIDIQSTDGPTAP
                     EESESND"
     gene            4089..>6138
                     /locus_tag="VFV87_04930"
     CDS             4089..>6138
                     /locus_tag="VFV87_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103129.1"
                     /translation="MLNRRRHFMTRVHKTVLWTLAACVFGYIAVHVLIVKAPGPALAQ
                     QPPEEPAGAIPLPEDDRGGPGEERAAPASALEPARALVAVCVGDKGEPLDDVIVDIVR
                     ETATESPFDPRVEPYGQATSKDGGRLSFALPPIRHRITGQAGIYILYFTAPGRPIKSV
                     VVENDTALPKEIAIPKESAEHVYAPTHHIVVAKSKGISISPLHSAIRAFTKDGKIYRV
                     VEFVDERTGVDKAPGKQTASGSWKPSPANPEDDVASMTIPKTGNGGRYGAGYGGSMAD
                     SGMPMSPPAAQDRITSARQKLTDARTDKEKQAARAQLRKLLAEIFAQDMQMREQQAAE
                     IESRLANLRRQYQAREKVKEEIIDLQLKVIEQDAAGLGFPGGGPGFPGGLPRSGVGGS
                     GFQSGGQGAMTAPQPAGRRSGVSVERPLAEFKQQQALIGELNKYGHFAESTDGKLYAH
                     VSSNHPRGPSFIFVVDSQTGKLVGTAAVNSVVGPILFTKEGLASRENDLLELRIRLTR
                     RAGPDGDVFEVGSADLNPFDPMTVALDPEFLKKYSSKPQARVGELAARGHFVGSPDGK
                     LYAYVETSGDNMPERSALVRVCDVRTGQRVAGTRIKPPVGKLRFFNHGVFTEDADGKL
                     TLRIRLNRRTGPEGDVFEVGSADLNPVDPLTVITAGGDVVELPGTANGATHSLRADMA
                     A"
BASE COUNT         1186 a         1832 c         1968 g         1152 t
ORIGIN      
        1 acaaagactc ttggaccgat gacgacttgg acacgttcgt gcgagaatcg ctgatgcgcc
       61 gcgaggcgtc ggaggggccg acctctgttc acgttgagca agttcgtgag caactactgg
      121 cacgctgtgc gccgcttgta caagttcggc gcagtcgccg ctacctgctc cgtgggctcc
      181 tcgctgccgg cctggctgct gggctgctca tggccatttt gctcgtccgg caaccgacgg
      241 cgggggcctg ggaagagcta cgagcggcct tgggtaacgt ggcgtgggtg catgtccaaa
      301 tgacggggcc ggacgggagg aaggtggagt cctggctctc gagccggcgc agcgtcgccg
      361 cggcgcgtgt ggagaagtgg gttgtttatg acgacttccg gcagggaatc cggtacgagt
      421 accggccgga cgaaaagaag ctctatcgcc tagctcttgg cgattccctg actggctcgt
      481 ttcgcgacgc ggtcgacgtg ctggcccaga tcatccgcaa cgatgaggaa ctctcggtcg
      541 gcaccggggg cccagaaatc gtccagcaat ccaaacgcac cgtatacgag gggagtgcgg
      601 agagtgtcga atacacggtg accacgcggc tggcaggggg gcgcaaggtc acgacaatcc
      661 tgcgcgtcga tccgcagact cacttgccgc agtcggctac cattcggcag tcggaaggga
      721 gcgagttgtt gtgccgattc gattatccag aattcgggcc cgaggacgtt tatgcgctgg
      781 gagtggccaa gaacgcgata gtggttgatc gcgtgccgtc cgccgatttg cagcgcgttg
      841 cgaccggcgt gctgaacggt cgtcgccaat tcgacgacta ttattgtctg ctcgtgcatt
      901 cctcggcgaa ccaacactgg tcccaagcga gtcgcatgta tcgcacttgg cgcaagggag
      961 attgctggcg aatcgagcag tcgtttaccg atcccgaaga tgacgaggcc ctggcgcgcc
     1021 aaccattgcc gccggacgac gtggaccgca aggcctattg gttccagcgc gccaaggaga
     1081 cacggttcta ccccgttttg gtctgcgacg gccagcacat ttggcgtttc aagcaggagt
     1141 tcgaccaacc gacggagaaa cgattcacct tcaaaacgac gggcgcagag cgagatcccg
     1201 tggatttcga ccagtccttc gtcatgctgc ccctgtttac gccggcaccg gaacttgagg
     1261 gccacagtgc gcccttggga gggacgagcg cgaaagtggt cgagcgtctc aatccccacc
     1321 ctgatcaagg accggcggga accgtgctgg tcgaacgcga atacatcggc cggcgcggcc
     1381 agccccagaa gcaagacctg gaccgctact gggtcgaccc agtgaacggc tacttggtga
     1441 tgcgccatga gatggtggac ctcgatcatg atccgccgca gatcatccaa tcgcgggaga
     1501 tcgctgactg gaaacgaagc ccgcgcgggt ttttctatcc cgtggagctg aagtgggacg
     1561 aatctggaat cgcccactat tacttggact tcgtgacgcc cgacgccgcc atgttcagcc
     1621 cgaaagcaac ggaatcgata aagcagagag tggcgacgcg ctgatgggac aatccttgat
     1681 tcgtgccatt gtctgcccct tggatcggcc atttgcgaaa cgaatatctc gccaactgcg
     1741 gcggtgtccg tctcgtagga cagcactgcg cgtgattgcg cccgccgtaa cccgcgccgc
     1801 ggtggttcga ctgccacttt gctaacacgg cgtctagtcc gatccattcc cggaattgcc
     1861 agaatgattg aaaggttctg ccagccggcg tgttctactt actggggtgg gaccatactc
     1921 gagttggcgt ttcgaatcgt gccaccacca ccggaaaccc gtgaaacgct gatccgacgc
     1981 ctgccgaatg cggcagatgt cgaggcgtgg gaggcgtttg tggagattta cgagccgctc
     2041 ttgtttcggc tggcacgcgg tcgaggaatg cagccggccg acgcggagga ctacgtccag
     2101 gaagtgctgg ctgctgtggc gagaaacatc gatcgctggg tggccagtga aacccgcggc
     2161 ccattccgcg cctggctgtt tcggattgcc ttcaatttgg gcgtcaattt tgtaacccga
     2221 ccgatgcacc agcggctggg cagcggcgat agccagatcg cacggatgct ggaagaagtg
     2281 ccagcggtgg cggcggattc gtcagcgctg ttcctgcagg agtatcggcg cgagctgttt
     2341 cgctgggcgg cggagcgggt gcgcgagcag gtgtccccgc aacaatggat ggtgtactgg
     2401 ctgaccagcg tcgaggagcg gcccatcgcc gaagtcgcga aggaatacgg tatgagtctc
     2461 ggcagcgttt acatcgcccg cagccggatt accaagcgca ttcgcgagat gattcgagag
     2521 catgaggagc gatcccaatg aattccagct ggaacccttg ccaggatgcc catctcgatg
     2581 tcctgctgga cggcgatgag gattcggagc agttcctccg cgcggcgagc cacgtcgagt
     2641 cctgtcccgc atgccagcgg cggctgactg agctgtcggg cgacgcgaag tattgggccg
     2701 tccagcgcga gatgctgcgg ccggctcccg gtgatcagcc gcagtccggc ctgctgtgtc
     2761 gccatcgttc gaccgtgtct gcctggtgtc gccgccccgt cgtagatgaa tcgagtgtgc
     2821 aagagttgct cgccgcgccg tcgcatccag agatgctcgg ccggctgggg cggtacgaga
     2881 tcgaacggat catcggcacc ggcggcatgg ggattgtgct gaagggattt gactcggaat
     2941 tgaaccggcc cgtggcgatc aagctgctcg cgccgcacct ggcgcgcgtg ggcgcggccc
     3001 ggcagcggtt tgcccgcgaa gcccgtgcgg cggcagcggt cgtgcatgag catgtcgtac
     3061 caatccacaa cgtcgaatcg gagcacgcaa ttcccttcct ggtgatgcag ttcattcccg
     3121 gcgaatcgct gcaagcgcgc gtggagcgcg aggggccgct cagcgtcgct gagatattgc
     3181 ggatcggcag gcaggcggca gccggcctgg cggcggccca cgctcaaggg ctggtgcatc
     3241 gcgatgtcaa acccgccaac atcctgctgg aaaacggcgt cgatcgggcg tatctgaccg
     3301 actttggcct ggcccgcgcg tcggacgatg ccagcctcac ttataccggc gtcgtcgccg
     3361 gcactccgca ctacatgtcg cccgaacagg ccgacggcca gccgctcgat cggcgttcgg
     3421 acctgttcag cctgggctcg gtgctgtact tcatggcgac cggccatccg ccgtttcgag
     3481 ccgaccggcc gatggcggtg ctcaagcgga cgtatcacga tccgcaccgc cccgcgtggc
     3541 aatgcaacgc cgaaattccc gattccctgt cccagatcat cgaccgcttg ctccagaaga
     3601 aaccggcttc ccggtttgcc agcgccggtg acctggaaca agccttggct gcggtgctcg
     3661 tcgaagtgca acaggggaga cttgggcgtc gccccaggta tcgttggcgg cgagcccaat
     3721 gggcaattgt gctcggcgtg gttgcagtcg ttgccctcgg tcttgccatc ggcccgttcc
     3781 ttatgcagcc tgcatccaac caggccaatc caaacgcaag cgttctaccg gcaggaggac
     3841 aacccaccga cgtagccggg ccagaccacc agtccgccgc aaccgaactg aatgccgtcg
     3901 atcccagccc gctgtctgcg gcgcagtccg aattcgactc gctttctgga atgctggacg
     3961 cgctggagac aacgcctttt ccggagaccg tttttggcgc ccaggagtct ccaggggaaa
     4021 acagaatcga catccagtcc acggatggtc cgacagctcc cgaagaaagt gagtcaaatg
     4081 attagcgctt gctcaatcga aggagacatt tcatgacccg cgttcataaa actgtcttat
     4141 ggactctagc ggcgtgcgtg ttcggctata tcgccgttca cgtcctgatc gtcaaagcgc
     4201 ctggccccgc gctggctcag cagcccccgg aggagcccgc cggagcgatc cctttgccgg
     4261 aggacgatcg cggcggtccc ggcgaagaac gcgccgcgcc ggcgtcggcg cttgagccgg
     4321 cacgtgcgct cgtcgcagtc tgcgtcggcg acaaggggga gccgctggac gacgtgatcg
     4381 tggatatcgt ccgcgagacg gcaactgaat cgccctttga tccgcgcgtc gaaccctacg
     4441 gccaggccac ttccaaagac ggaggccggc tgtcgttcgc attgccgcca attcggcaca
     4501 ggatcacagg ccaggcgggc atttacatcc tgtatttcac ggccccaggc cggccgatca
     4561 aatcggtggt cgttgagaat gacaccgcac ttccaaagga aatcgccatt cccaaggagt
     4621 ccgcggagca tgtttacgct ccgacgcatc acatcgtggt ggccaaatcc aagggaattt
     4681 cgatcagccc gctccactcg gcgatcagag cgttcacaaa agatgggaag atttatcgag
     4741 tggtggaatt tgtcgatgag cgcactggag ttgacaaggc accgggtaaa cagactgcgt
     4801 ctggatcctg gaaaccttcc ccggcaaatc ccgaggatga cgtcgcgtcg atgaccatcc
     4861 cgaaaactgg caatggcggg aggtacgggg caggttacgg gggcagtatg gcggatagcg
     4921 gcatgccgat gtctccgccc gcagcacaag atcggatcac gtccgctcgc cagaaactga
     4981 ccgatgccag gaccgacaag gaaaagcaag ctgctcgtgc gcagcttcga aagctcttgg
     5041 ccgagatctt cgcccaggac atgcagatgc gcgagcagca ggcggcggag attgaatccc
     5101 gcctggccaa tctgcgccgg caatatcagg cccgcgagaa ggtcaaggag gagatcatcg
     5161 atttgcaact caaggtgatc gagcaggacg ctgccgggct ggggtttcca ggtgggggac
     5221 caggtttccc aggtggcttg ccgcgatctg gagttggcgg gtcgggattt cagagtggcg
     5281 ggcaaggtgc catgactgcg ccccaaccgg ccggtcgcag gagtggtgtt tcggtcgagc
     5341 ggccgctcgc ggagttcaag cagcagcaag cgctgatcgg cgaactgaac aagtacggtc
     5401 attttgccga gtccaccgat ggcaagctct acgctcacgt ttcctccaac catccccgcg
     5461 gcccgtcgtt catctttgtc gtcgattccc aaacgggaaa actggtggga acggcagccg
     5521 tcaattcggt cgtcggcccg attctattca ccaaggaagg tctggcctcg cgcgaaaacg
     5581 acctgcttga actgcggata cgcctgactc gccgcgcggg gccggatggc gatgtgtttg
     5641 aggtcggttc ggcggatttg aatccgttcg atcccatgac cgtcgccctc gatccggaat
     5701 tcctgaaaaa gtactcgagc aaacctcagg cgcgggtcgg cgaattggcg gcgcgtggtc
     5761 acttcgttgg gtcgcccgat ggcaaactct acgcatacgt cgagaccagt ggcgacaaca
     5821 tgcccgaacg atccgccctg gttcgcgtgt gcgacgttcg aaccggacaa cgtgtggctg
     5881 gcacccggat caagcctccc gtggggaaat tgagattctt caaccatggg gtcttcacgg
     5941 aagatgctga tggcaagctc accttgcgaa tacgactgaa tcgccgcaca gggccagagg
     6001 gcgatgtgtt tgaggtcggt tcagcggatc tgaatccggt cgatccgctg actgtcatta
     6061 ccgcgggtgg agacgtcgtt gagctacctg gcacagctaa tggcgcgaca cattccctgc
     6121 gcgccgatat ggcagccg
//
LOCUS       DASUGB010000255         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1836013, whole genome shotgun sequence.
ACCESSION   DASUGB010000255 DASUGB010000000
VERSION     DASUGB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836013"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFV87_04935"
     CDS             <1..471
                     /locus_tag="VFV87_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103130.1"
                     /translation="LALNVAVRGKEQPPWCRDSKIEDSDGVQLWIDTRNTQNIHRAGR
                     FCHRFAFLPVGSGRRLDEPTAALLAINRAKESPREIDPRQLLVRAERTTDGYLLQAHV
                     PAETLTGYSPADQPALGFTYAVMDRELGWQTFSVGQELPFSEDPSLWGTLDLVR"
     gene            530..2224
                     /locus_tag="VFV87_04940"
     CDS             530..2224
                     /locus_tag="VFV87_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5103131.1"
                     /translation="MPRSLAFPCALVAVFCSIPWAIIAAEPAFRFRDAGDEAGLFPAL
                     AGIKGHGAGWGDVDGDGWADLLVGTFDTGDSKPCLFFRSDRGRLRHADQAATRISGRV
                     TGIVFADLDNDGDLDAYLGSMPAAADSRLALKEGHPLAACSLLANDGQGRFTDVSQAS
                     GACPAAFGGRSVAVLDFDGDGLLDLLVGEDPIPGYNGSQTKSSRLFRNKGQLKFEDAS
                     RSAGIPEGFPGLGVAAADVTNDGWPDIFLASSGGGNVLLVNDGHGTFREPPGGREVFA
                     WKDAIGDDMCCGVCIADVNRDGLLDIVLGQHYSRPWAKPVANRLYLNRGIEAAGPRFE
                     DVTERVGLVPLSMKSPHVEIQDFDNDGWPDIYASMVKFDASGRPHPVIFRHLGLERGL
                     PKFGCDALSVNDFPTAEDLAVKNTGKFFEKMIADHKLIYMAPGPTCDYNRDGKLDMLL
                     PSWWPEARSLLLAGETPGGHWLQVAVRGPAGVNRQGIGARVLVYPPGKLGQAGQLQAS
                     REIAVGFGYASGQEAIAHVGLGELTACDLAILLPHGKGRIERRSIQADQRIVIDKP"
     gene            2401..>3144
                     /locus_tag="VFV87_04945"
     CDS             2401..>3144
                     /locus_tag="VFV87_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103132.1"
                     /translation="MRPAIVRLALTFALLAGAPAIAAGQEFRIDTELFIGKQKEPRVE
                     TLTLFSGGMIYDFLLSRPEEISLFDPVRGRFTLLDPARKLRCGLSTQDVLNYCLALET
                     HAAESKDPLFQFAASPQFTPQVEEFAENGQQRTRLVMAGKPLEYAAVAYHPEQPEAVS
                     AFRSFTDWYARLNAMRPGGLPPGARLELNRALAERGLVPLEITCTLAAPNVLASKTSL
                     RSRHLVTWTLSGEDRKRIERAGNYLVEFQA"
BASE COUNT          516 a         1067 c         1032 g          529 t
ORIGIN      
        1 ctggcattga acgtggccgt ccggggcaag gagcagccgc cctggtgccg cgattccaag
       61 atcgaggaca gcgacggcgt gcagctctgg atcgacaccc gcaacacgca gaacattcac
      121 cgggcgggcc gcttctgcca ccggttcgcc ttcctgccgg tcggctcggg ccgtcggctg
      181 gacgagccga cagccgccct gctggcgatc aaccgggcga aggaaagccc gcgggagatc
      241 gacccgcggc agcttctcgt gcgggccgaa cgaacgaccg atggctacct gctgcaagcg
      301 cacgtgccgg ccgaaacact taccggatac agcccggccg accaaccggc gctcggcttt
      361 acatacgccg tgatggaccg cgagctgggc tggcagacat tcagcgtcgg gcaggagctg
      421 cctttctccg aagaccccag cctgtgggga acgctggatt tggtgcgcta accggcccag
      481 aatgtggttc gctggtcccg atccacgcgc gcccagcggg agccccgcca tgccacgatc
      541 cctcgctttc ccctgtgcgc tcgttgcggt cttctgttca attccctggg caataatcgc
      601 cgcggagccg gcgttccgct ttcgcgacgc cggcgacgag gcggggctct tccccgcact
      661 ggccggcatc aaggggcacg gcgcgggctg gggcgacgtc gatggcgacg gctgggccga
      721 cctgctggtg gggacgttcg atacgggcga ttccaagccg tgccttttct ttcgcagcga
      781 ccgcggtcgg ctgcgccacg cggaccaggc ggccacgcgg atttcgggcc gtgtgaccgg
      841 catcgtcttt gccgatctgg acaacgacgg cgacctggat gcgtacctgg gcagcatgcc
      901 ggccgccgcc gacagccggc tggctctgaa ggaagggcac cccctggcag catgctcgct
      961 cctggcgaac gacgggcagg gccgcttcac cgacgtctcg caggccagcg gcgcgtgccc
     1021 tgcggcattt ggcggccgca gcgtggcagt gctcgacttc gacggcgacg gcctgctcga
     1081 cctgctcgtc ggcgaggacc cgattcccgg ctacaacggc tcccagacga aaagctcgcg
     1141 gctgtttcgc aacaagggac agcttaagtt tgaggatgcc tctcgatcgg ccggcatacc
     1201 cgaggggttt cccggcttgg gtgtggcggc cgcggacgtg acgaacgacg gctggcccga
     1261 catttttctg gcgtccagcg gcggcggcaa cgtgctcctc gtgaacgacg gccacggcac
     1321 gttccgcgag ccgcctggcg ggcgggaagt gttcgcctgg aaagacgcga tcggcgacga
     1381 catgtgctgc ggcgtgtgca tcgccgacgt caatcgcgac ggcctcttgg acatcgtcct
     1441 tggccagcac tattcgcggc cctgggccaa gccagtcgcc aatcggctgt atctcaatcg
     1501 tggcatcgag gcggctggcc cgcggttcga ggacgtgacc gaacgggtcg gcctagtgcc
     1561 gctgagcatg aagtcgccgc atgtggagat ccaggatttc gacaacgacg gctggccgga
     1621 tatttacgcg tcgatggtca agttcgacgc cagcggccgg ccgcatccgg tcatctttcg
     1681 ccaccttggc ctcgaacgcg gcctccccaa gttcggctgc gatgccctga gcgtaaacga
     1741 ctttccaacg gctgaggatc tggctgtcaa aaacaccggc aaattcttcg agaagatgat
     1801 cgccgaccac aaactgattt acatggcccc agggccgacg tgcgactaca accgcgacgg
     1861 caagctcgac atgctactgc ccagctggtg gcccgaggcc cggtcgttgc tgctggcagg
     1921 cgaaacgccc ggcggccact ggctgcaagt cgccgtccgc ggccccgcgg gggtcaatcg
     1981 ccagggcatc ggcgcccgtg tgctcgtcta tcctcccggc aagttgggcc aggccgggca
     2041 actgcaagcc tctcgcgaaa tcgccgtggg ttttggctac gcatcgggcc aggaggcgat
     2101 tgcccacgtc ggcctgggcg aactgacggc ctgcgacctg gcaatcctcc tgccgcacgg
     2161 caagggccgg atcgagcggc gcagcatcca ggcggaccag cggattgtga tcgacaagcc
     2221 ttgaggccaa ggcgcagcgc cctgtgcgga cggcgcaacg acgaccaggc cggtttcacc
     2281 gcggtcgtgc caccgcgcgc tgctagcgtg ccggtgcaat tcccctggac gcccgaggcg
     2341 ctgcgcgggt atcgttcgcg ccctttgtgc ttgactggct gcgcctcgag gaaccttgcc
     2401 atgcgtcccg cgattgtccg actggctttg acgtttgcgc tgctggccgg agcgccggcc
     2461 atcgccgccg ggcaggaatt ccgcatcgac accgagctgt tcatcggcaa acaaaaggag
     2521 ccgcgggtcg aaacgctgac gctgttttcc ggtggcatga tctacgactt cctgctgtcg
     2581 cgtcccgagg agatctcgct gttcgacccc gtgcgcggcc gcttcacact gctcgatccg
     2641 gcgcgaaagc ttcgctgcgg cctgtccacc caggatgtgc tcaactactg cctggccctg
     2701 gaaacgcacg ccgccgagtc gaaagacccg ctcttccagt tcgcggccag tccccagttc
     2761 actccccaag tcgaagagtt tgcagaaaac ggccaacaac ggacgcggct ggtgatggcc
     2821 ggcaagccgc tcgagtatgc cgcggtcgcc taccatcccg agcagcccga agcggtgtcc
     2881 gcgtttcgga gctttaccga ttggtacgcc cgcctcaatg cgatgcgccc gggcggattg
     2941 cctcccgggg cccggctgga gctgaaccgg gccctggccg agcggggact ggttccgctg
     3001 gaaatcacct gcacccttgc cgcccccaac gtgttggcca gcaaaacctc gctccgcagc
     3061 cgtcacctgg tgacctggac cctatcgggc gaagatcgca agcgaatcga gcgggccggc
     3121 aactacctgg tcgaattcca ggcg
//
LOCUS       DASUGB010000256         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_446698, whole genome shotgun sequence.
ACCESSION   DASUGB010000256 DASUGB010000000
VERSION     DASUGB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446698"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..>2516)
                     /locus_tag="VFV87_04950"
     CDS             complement(60..>2516)
                     /locus_tag="VFV87_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5103133.1"
                     /translation="GHWGAFAASALFTWLGLGAYYLVLSLAILDVQLLRRREIDMPAL
                     RALGWVASLWGICTLAALAFEWLSLGPVIGAGGYLGALGKSLASMHFAWTGGLILAAS
                     LTLGGLLLATDYALISAVLFAMRLLARRREETAASSGGKGTAGKRSRSDLDDEPFGEQ
                     SAVPIKIRGKPAQETAASQDAAEGDEEDDDQPVTLRVVNPADSKSDQSEGQATRQDKP
                     ENPLRVKSPADRKKEAADRERAELMRKMNEASPLADAEDYELPTVDLLLPSDDVNYEA
                     QTTEVRRKAKILEKTFADFGFTVKVVEIETGPVIAQYEVELEAGLRLAKITGLADDLA
                     IALRVPSVRIVAPIPGKNTVGIEVPNEQRQVVRLREVIEEANGKVKKMRIPLFLGKDV
                     SGNALTVDLSTLPHLLIAGRTGTGKSVCLNAIISSILMTRRPDEVRMLMIDPKMVELS
                     GYGRLPHLMHPVVTDMRKAEAILAWAVEKMEERYALLARAGVRHISSYNQLGEEELME
                     RLQPENDEERASIPQQLPFIVIVADEMADLMMTSGKEVEQHIIRLAQKSRAVGIHLIL
                     ATQKPTVDVITGLIKSNLPARIAFQVASRTDSRVVLDEMGADKLLGNGDMLFLWPGTS
                     TLLRGQGTYLSDEEINAIVDCCSRGEQNFVQELVNIKVKDPEADEDDAKPTALQKRDE
                     LYEQAVDLVVREGRGSVSLLQRALGIGYGRAARLIDFMAEDGIVGQYNGSQAREILIS
                     LADWERMQGGTSEADDAPPTTAPAKKRNRILPDVPRGDTHEDTEESDSEEASDESPWE
                     DSAEDSADEDADDGYRAEIA"
BASE COUNT          394 a          839 c          769 g          514 t
ORIGIN      
        1 aaagggtccg caagccaggg ctcagtccct cgctggcgcg ccgggttgac gggcagattt
       61 caggcgattt ccgcgcggta gccgtcgtcc gcgtcttcat cggcggagtc ctcggcgctg
      121 tcttcccatg gcgattcgtc cgaagcttcc tcgctgtcgg actcttccgt gtcctcatgc
      181 gtgtcaccgc gcgggacgtc gggcaggatc ctgttgcgct tcttcgctgg agccgtcgtg
      241 ggaggcgcgt cgtcggcctc ggaagtgcca ccttgcatcc gctcccaatc ggcaagggag
      301 atgaggatct cgcgggcctg gctgccgttg tactggccga cgatgccgtc ttcggccatg
      361 aaatcgatca gcctggccgc gcggccatag ccgatgccca gggcccgttg caggagcgac
      421 acgctgcccc ggccttcgcg gacgaccaga tcgacggcct gttcgtagag ctcgtcgcgc
      481 ttctgcagcg cggttggctt ggcgtcgtcc tcgtcggctt cggggtcctt gaccttgatg
      541 ttgaccagct cctgcacgaa gttctgctcg ccgcggctgc agcaatcgac gatcgcgttg
      601 atctcctcgt ccgaaaggta ggtgccctga ccgcggagca gcgtgctggt tcccggccag
      661 aggaacagca tgtcgccgtt gcccagcagc ttgtcggccc ccatttcgtc gaggacgacg
      721 cggctgtcgg tgcggctggc aacctggaac gcgatgcggg cgggcaggtt cgacttgatc
      781 aggcccgtga tgacgtccac cgtcggcttt tgcgtggcga ggatcaggtg aatgccgacg
      841 gcccggctct tctgggcgag gcgaatgatg tgctgctcga cctccttgcc gctggtcatc
      901 atcaggtcgg ccatttcgtc ggccacgatc acaatgaacg gcagttgctg cgggatcgac
      961 gcccgttcct cgtcgttctc cggctgcagc cgctccatca gctcttcttc gccgagctgg
     1021 ttgtacgagc tgatgtggcg cacgccggcc cgcgcgagca gggcataccg ctcctccatt
     1081 ttttcgacgg cccaggccag gatcgcctca gcctttcgca tgtcggttac cacggggtgc
     1141 atcaaatgcg gcaaacggcc gtagcccgac agctcgacca tcttcggatc gatcatcagc
     1201 atccgcactt cgtccggccg gcgggtcatc aggatcgacg agatgatcgc attgaggcac
     1261 acactcttgc cggtgccggt gcggccggca atgagcaggt gcggcagcgt cgaaagatcg
     1321 accgtcaggg cattgccgct cacatccttg cccaggaaga gcggaatccg catcttcttc
     1381 acttttccgt tggcctcttc gatgacctcg cggaggcgca ccacctgccg ctgctcgttg
     1441 ggcacttcga ttccgacggt gttcttgccg ggaattggcg cgacgatgcg gacgctcggc
     1501 acgcggaggg cgatcgccag atcgtcggcc aggcccgtaa tcttcgccag ccgcagcccc
     1561 gcttcgagct cgacctcgta ctgggcgatg accgggccgg tttcgatttc gacgactttc
     1621 accgtgaagc cgaaatcggc gaacgtcttc tcgagaatct tggccttgcg gcggacttcc
     1681 gtcgtctgag cctcgtagtt cacgtcatcg ctcggcaaca acagatccac cgtcggcagc
     1741 tcatagtctt ccgcgtcggc cagcgggctg gcctcattca ttttccgcat caattcagcc
     1801 cgctcgcggt cggctgcttc tttcttgcgg tcggccggac tttttacgcg gagtggattc
     1861 tccggcttgt cctggcgcgt cgcttgtccc tctgattgat cggacttgct atccgcaggg
     1921 ttcaccacgc gcagcgtaac tggctgatcg tcgtcctctt cgtcgccttc tgctgcatcc
     1981 tgactggccg cagtttcttg cgccggtttg ccgcgaatct tgatcggcac ggctgattgt
     2041 tcgccaaaag gctcgtcgtc caaatcgctg cggctgcgtt tgccggcagt gcctttaccg
     2101 ccgctgcttg ctgcggtctc ctcgcggcga cgagccaaga gccgcatcgc gaatagtact
     2161 gcgctgatca gggcatagtc cgttgccagc agcagtccgc ccagcgtaag gctggccgcc
     2221 aggatcaggc cccctgtcca ggcaaagtgc atcgaggcca gcgacttccc gagcgccccc
     2281 agataccctc ccgcgccaat cactggacca agcgaaagcc actcgaaagc cagtgcagcc
     2341 agcgtgcaaa tcccccacag cgacgccacc cagccgagcg cccgcagcgc cggcatgtca
     2401 atttcgcgcc gtcgcagaag ctgaacatca aggatggcga gcgacagaac aaggtaatac
     2461 gcccccaggc cgagccaggt gaacagcgcg ctcgccgcga aggctcccca gtggcc
//
LOCUS       DASUGB010000257         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_724376, whole genome shotgun sequence.
ACCESSION   DASUGB010000257 DASUGB010000000
VERSION     DASUGB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724376"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFV87_04955"
     CDS             <1..337
                     /locus_tag="VFV87_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103134.1"
                     /translation="EAAPTLQGLTEAALSADLRILGSATIETGGGHDCVELFDVFVGT
                     TLTVSLGAGNDCLCLFDVTSAIAIFLGGAGFDEILFDFDGLRDPATGYLSYTQLQFEF
                     QGACFDDVD"
     gene            complement(351..1295)
                     /locus_tag="VFV87_04960"
     CDS             complement(351..1295)
                     /locus_tag="VFV87_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX5103135.1"
                     /translation="MTPEIVTLRDAITGSSAEILVSQGFNCFRFTAIAGGQPVEVIYA
                     PPDFSRGQSRPSSGGIPILFPFPGRIPGTILRWEGKEYQLEAGDAFGNAIHGFVHARP
                     WRVVEQSESRVVGEFHAWRDDPSLQARWPADFRITATYALAASELRMEYLLENPGEAP
                     LPCGLGVHPYFRLPLGGSSRDACIVKLPVSSRWELKDMLPTGRRLELEDAAAYQEGRT
                     FGELKLDDVFSGLVPDSSGELAASITDPGGCRLTIRFGQPFRECVAYTPPHREAICIE
                     PYTCVPGAFELEARGIDAGLQILPPGAALSAAMNLNVS"
     gene            complement(1306..2247)
                     /locus_tag="VFV87_04965"
     CDS             complement(1306..2247)
                     /locus_tag="VFV87_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103136.1"
                     /translation="MSLLIAVHLLCVNVAAGGPLVAAWLDWRGSRDEAAGRAAKWLAG
                     RSVAGLLAGAALGVLIGWLKWDADYRSLWTGPLSYKMHWAGIEAVFSLVLMIGWWLWT
                     PGRAGGSAAASLIRGLLAFLASTNLLYHFPVLFFVAARLHDAGQTSGETIRGAAFRQL
                     MIVDDAPAMAVHVTLASVAVAATMLLGLSLRWRRHSDEAGAYKVAVWGGRWALVATVL
                     QLPVGLWALATMPRSSQAQIMGGSTLGFVLFLVSIAAALWLMRELVNVALGDGTRPAL
                     VRVMVLMLATVVLMTAMQQQTRGGASVSPERAAESTE"
     gene            2580..>3415
                     /locus_tag="VFV87_04970"
     CDS             2580..>3415
                     /locus_tag="VFV87_04970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5103137.1"
                     /translation="MLSAVIPVYNEVESLAALHGELAEVAQQHGYDLEIIFVDDGSKD
                     GSWEAIEKLAAADSRVKGIRFRRNFGKAAALSAGFAAASGELVVTLDADLQDDPHEIP
                     RFLEAIDSGLDCVSGWKKLRHDPWHKVGPSRVFNWLVGRLTGVKLHDHNCGFKCYRRE
                     IFDEVRLYGELHRFVPVLAAARGWQVGEIVVNHRARQFGRSKYGVTRIVKGFLDLLTV
                     YFLTGFDQRPQHLLGTLGILSFLVGFAGLAYLVLTWILTNLTDWNTRFPDWAFKPLHE
                     RPL"
BASE COUNT          643 a         1169 c         1024 g          579 t
ORIGIN      
        1 cgaggccgca ccgacattgc agggcctgac ggaggcggcg ctcagcgccg atttgcgcat
       61 cctcggctcc gcaacgatcg agaccggcgg cggccacgat tgcgtcgagc tgttcgacgt
      121 cttcgtcggc actacgctaa ccgtcagcct cggtgcaggc aacgattgcc tgtgcctctt
      181 cgacgtgact tccgcgattg ctattttcct cggcggcgcc gggttcgacg agatcctgtt
      241 cgatttcgac ggactccgag atccggcgac cggctacctg tcctacaccc agttgcaatt
      301 tgagtttcaa ggtgcgtgct tcgatgacgt ggactgagcg ctgacgggcc tcacgacacg
      361 ttcaggttca tggctgccga gagcgcagct ccaggcggca atatctggag ccccgcgtcg
      421 atcccacgcg cctcgagctc gaaagcgcct ggcacgcacg tgtagggctc gatacaaatc
      481 gcttcacggt gcggcggagt gtaagcgacg cattcgcgaa atggctggcc gaagcgaatc
      541 gtcaggcggc aaccgccggg gtccgtgatt gaagctgcaa gttcgccgct actgtctggc
      601 accaggcccg agaaaacatc gtccagtttg agttcgccga atgttcgtcc ttcttgatac
      661 gcagcggcat cctccagctc caaccgccgg ccggtcggca gcatgtcttt gagctcccag
      721 cgggacgaaa cggggagctt cacgatgcac gcatcgcggc tgctgccgcc aagcggaagc
      781 cggaagtagg gatggacgcc caacccgcac ggcagcggcg cttcgccggg gttttcgagc
      841 agatactcca tacgcagctc gctggcggcg agcgcgtacg tcgccgtgat tcgaaaatca
      901 gccggccaac gggcttgcag cgaaggatcg tcgcgccacg cgtgaaattc gccaaccacg
      961 cggctttcgg attgctcaac gacgcgccac ggccgggcgt ggacgaagcc gtggatcgcg
     1021 ttgccgaacg cgtcgcccgc ttcgagctga tattccttgc cctcccagcg aaggatcgtg
     1081 ccaggaattc ggccggggaa cggaaacaag atcggaatgc cgccgctgga gggccggctt
     1141 tggccccggg aaaaatccgg cggggcatag atcacttcga ccggctggcc gcccgcaatc
     1201 gcggtgaagc gaaagcagtt gaacccctgg ctgacgagga tttccgccga cgagccagtg
     1261 attgcatcac gaagggtgac gatttcaggt gtcatggcga gtgtttcatt cagtcgattc
     1321 ggcagcacgc tccggcgaga cgctcgcccc accgcgcgtc tgctgttgca tggcagtcat
     1381 gaggacgacg gtcgccagca tcagcaccat cacgcgcacg agcgccggcc gagtgccgtc
     1441 gcccagagcc acattgacaa gttcgcgcat cagccacagt gccgcggcga tggaaacaag
     1501 aaagagcacg aatcccagcg tgcttccgcc catgatttgt gcttgggaag accgtggcat
     1561 ggttgccagc gcccaaagcc ccaccggaag ctgcaggacg gtcgcgacca acgcccaccg
     1621 cccgccccac accgctactt tgtacgcccc ggcctcatcg ctgtgccttc gccaccgaag
     1681 cgacagcccc aacagcatcg tcgctgcgac ggcgactgag gccagcgtca catgcacggc
     1741 catcgccggc gcgtcgtcta ctatcatgag ctgccgaaag gccgcgccgc ggatcgtctc
     1801 gccggacgtc tggccagcgt cgtgcaatcg ggctgctacg aaaaacagaa ctggaaagtg
     1861 atagagcaga ttcgttgaag ccaggaaggc aagtaggcca cgaatgagtg acgctgcggc
     1921 actcccccct gccctgcccg gcgtccacag ccaccagccg atcatcagca cgagcgaaaa
     1981 gaccgcctca attccggccc agtgcatctt gtagctgagt ggtcccgtcc agagcgaacg
     2041 gtaatcggca tcccatttca gccagccgat gagaacaccc aaggctgccc cggccagcaa
     2101 ccccgcgacc gaccggccgg ccagccattt cgcggcccgg cctgccgcct cgtcgcggct
     2161 gccgcgccaa tcaagccacg ccgccacgag cggcccgccg gccgccacat tcacgcagag
     2221 caaatggacc gcgatcagca gactcatcgc tagggcagcc acgcctgatc ctcaactcgg
     2281 tcccgccggg aaaacaactg gcccagcatt atagcgcccg cggctcggca acagataggc
     2341 ttcgcggcag acgaaacagg gcccttcgat tattctggaa aggcaaccgc cagcgctggg
     2401 taggcgtcac cgttggccaa aggggtcggg agtcaattgc ggttacgact ttccacctgg
     2461 atgaccgtta ccgcaattga ctcccgaccc cctcgccgca gccggctcgg tggttgccca
     2521 caatcgagac gcccacccac cagcgccccc tcccctcacc tgccccgttt cccggcctca
     2581 tgctctctgc cgtcattccc gtctataacg aggtcgagag cctggcggct ttgcacgggg
     2641 aattggcgga ggtcgcccag cagcacggct acgacctgga aatcatcttc gtcgatgacg
     2701 gctccaagga cggctcgtgg gaggcgatcg aaaagctcgc ggctgccgac tcgcgggtga
     2761 agggcattcg cttccgccgc aatttcggca aggccgcggc cctctccgcc ggtttcgccg
     2821 ctgccagcgg cgagctggtc gtcacgctcg atgccgacct acaggacgac ccgcacgaaa
     2881 tcccgcgatt cctggaagcg atcgacagcg gcctcgactg cgtcagcggc tggaagaagc
     2941 tccgccacga cccgtggcac aaagtcggcc cctcgcgggt tttcaactgg ctcgtcggcc
     3001 ggctgaccgg cgtcaagctg cacgaccaca actgcggctt caagtgctac cgccgcgaaa
     3061 tcttcgacga agtccgcctc tacggtgagt tgcaccgctt cgtccccgtc ctcgccgccg
     3121 cccgcggctg gcaggtcggc gaaatcgtcg tcaaccaccg cgcccgccag ttcggacgct
     3181 cgaaatacgg cgtcacgcgg atcgtcaaag gcttccttga tctcttgacc gtctatttcc
     3241 tcaccggctt cgaccagcgg ccgcagcatc tgctcggcac gctcggcatc ctgtcgttcc
     3301 tcgtcggctt tgcagggctc gcgtacctcg tgctgacgtg gatcctgacg aacctgaccg
     3361 actggaatac gcggtttccg gactgggcct tcaagccgct gcatgagcgg ccgct
//
LOCUS       DASUGB010000258         4634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_584978, whole genome shotgun sequence.
ACCESSION   DASUGB010000258 DASUGB010000000
VERSION     DASUGB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584978"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..641
                     /locus_tag="VFV87_04975"
     CDS             186..641
                     /locus_tag="VFV87_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103138.1"
                     /translation="MKQTSTFFAALLALASVPALADDGQISKTALRSLGLGGMQVLSD
                     ADGMQIRGLSSNAASGGTSMIAGQLVFNDANGSQLVTGSDLNTSRSTAENAGLNAVSN
                     ATHSQGSGLILTLGPIVNFAGFQFSGALSGQAGQTTAPTLAGNGFALGF"
     gene            993..1409
                     /locus_tag="VFV87_04980"
     CDS             993..1409
                     /locus_tag="VFV87_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103139.1"
                     /translation="MALVAVPALAADGVPAAKLRALGLGGMQVMSDADGMQVRGMSSN
                     AASGGSSLIFGQLTFNDGVGTQFVVGSDFNVSRGTSENAGLNAVSTANSNHGSGLILT
                     LGPILNPGLAFQGALTGQAGQTTLPGLAGNGFATGF"
     gene            1537..1992
                     /locus_tag="VFV87_04985"
     CDS             1537..1992
                     /locus_tag="VFV87_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103140.1"
                     /translation="MKRASLMIAALVAMVSVPAFAEDGHVSKATLRSLGLGGMQVMSD
                     ADGMQVRGLSSNAASGGSSLVAGQLAFNDASGTQFVIGSDFNTSRATAVNAGLNASST
                     SSHNQGSGLILTLGPIVNAAGLQFTGTFAGQAGQVTAPGFAGSGFALGF"
     gene            complement(2085..2525)
                     /locus_tag="VFV87_04990"
     CDS             complement(2085..2525)
                     /locus_tag="VFV87_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103141.1"
                     /translation="MKFAPVFLLLAVAASPALAGDGQIPQSKLQRLGLGQMQTMSDSQ
                     GMEVRGQSSGVMAGGLSLVFGILQDPANPGNFVGATDLNTAMASAENAGLQVLSTSQV
                     TQGSAISTSLNVITPVSSFAGVLFGLGGNAAAPGIGGFSFGSAQ"
     gene            2980..4521
                     /locus_tag="VFV87_04995"
     CDS             2980..4521
                     /locus_tag="VFV87_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103142.1"
                     /translation="MRIQLQICVFAACALAALGAARGNDDSSEAFAVADPAETLHAAS
                     PIGASSMDGPAASNSLSTQDAEALAQWAERQAELAIINGESAEAVQEKKQLAVWARAQ
                     ADEAASPADIAPAAGFAADPLASPVVFNQAEALPGQAPILPRFRAGQPTFASQPPPGP
                     PATVDATLSDDGQEVVPTPTPVDGVIVDGYGSWDDIYGMCSVCCCSGLFLGVEGTFLA
                     PVGEPTTGVLLTDLVSGATYSGTSQPGLGAGVRTWLGLQNCNGTGFVVRYWTFGNQVI
                     NPDPVVPVNKQPAFHNSYFLNAYALDIEMIQRFCWHGTRWYATFGGRYARLERNQLVA
                     GYGTVGDVSLYGLAQAANEIEGAGFTTSLWATGPLCGCCLPCGFNWYGGARISILWAD
                     SLASAYTEANAAVKPPIGAAVANSIDAAFASKDHSETIFIAEAQAGIQYEHCLPCHNA
                     IFFARAGFEYQHWNTGDVFAGSNSFAFLAGGPPPFGGRVDAFANAHDGNLDLIGFVLG
                     AGLTY"
BASE COUNT          792 a         1481 c         1409 g          952 t
ORIGIN      
        1 caggctggcc agacgaccct gccgggcttc gcgggcaatg gcctggcttc tggcttctag
       61 tgaatcagtc gcagatggct tgagttagcg atgttgctag cagcagcttc tatggctcgc
      121 ccatcggacc gatgggcgag ccttttgcat tggcccacat ctatctctcc tggagttcct
      181 cactcatgaa acaaacctcg acgtttttcg ccgcactcct ggccctggcg tctgttccgg
      241 cattggccga cgatggccag atctcgaaga cggccctccg gtcgcttgga cttggcggaa
      301 tgcaagtcct gtcggacgcc gacggcatgc agattcgcgg tctgagctcg aacgccgcct
      361 cgggcggcac cagcatgatc gccgggcagc tcgtgttcaa cgacgccaac ggctctcaat
      421 tggttacggg ttcggatctc aacacctcgc gcagcactgc tgaaaatgcg ggcttgaatg
      481 ccgtttccaa cgccacgcac agccaaggat ccggactgat tctgactctt ggcccgatcg
      541 tcaattttgc cgggttccag ttcagcggcg cgctcagcgg ccaggctggc cagacgacgg
      601 cgcccactct tgcaggcaat ggctttgcgt taggcttcta gcacacaagc ctgccgcgtc
      661 cattcaacaa acgctgtgat ggattcctga ggctcgctca tcacgtccga tgagcgagcc
      721 tttttcattc cggttgcccg tagaagctcg ccaataccac cccctcttat agcgggtgtc
      781 ggcaattcca atggattcta ctgattttgt gaatttttag ggtggcaatt gacatgcggg
      841 ttgccgcgtt taaattgtcc gtactttgcg gcaggggaaa gatcctccag atcttgctgt
      901 ttccctagtc tgcgaacttg ttcttgcttc cctattttct caactctgtt ggaggttctg
      961 gtatgaagtg cgcttcgctg gtcgctgctg tgttggccct ggttgccgtt cctgcgctgg
     1021 ctgctgacgg tgtgcctgcc gccaagcttc gggccctggg actgggcggg atgcaagtca
     1081 tgtccgatgc ggacgggatg caggtccgcg gcatgagctc gaatgcagcc tcgggcggct
     1141 cgagccttat ttttggacaa ctgacgttta acgacggcgt cggcactcag ttcgtcgttg
     1201 gctccgactt caacgtctcc cgcggcacgt cggagaatgc cggcttgaat gccgtttcca
     1261 cggccaacag caaccatggc tccggactga tcctgacgct gggcccgatt ctcaacccgg
     1321 ggttggcgtt ccagggcgcg ctcaccggcc aggccggtca gacgacgttg ccgggcctcg
     1381 cgggaaatgg cttcgccacc ggcttctaac gcatccctcg cacggtacat gcataggcgt
     1441 gagtgccaac aaaaactcct ctgggctcgc ccttcgaatc gatgggggag ccctttcacc
     1501 gtcttccatt ctcacttcgc ctggagattc tcaaccatga agcgcgcttc tttgatgatt
     1561 gctgccttgg tagcaatggt ttctgtccct gcttttgccg aggacggcca tgtctcgaag
     1621 gctaccctgc ggtcgcttgg tttgggcggc atgcaagtca tgtccgatgc cgacggcatg
     1681 caggtccgcg ggctcagctc gaatgctgca tctggcggct cgagtttggt cgccggccag
     1741 cttgcgttta acgatgcgag cggcacccag tttgtcatcg gttccgattt caatacgtcc
     1801 cgcgcgacgg ccgtgaatgc aggcttgaac gcctcatcga cctcgtcgca caaccagggc
     1861 tcgggcctca tcctcaccct tggcccgatt gtgaacgcgg ctgggcttca attcaccggc
     1921 acgtttgccg gccaggccgg ccaggtcacc gcgccgggat ttgctggcag cggtttcgcc
     1981 ctgggcttct aagccaagaa gcttaaacta cgtttgttca aaataaaccc ccggaccgaa
     2041 agtccggggg ttctttcatg cgcgaactgg caaccgacgc tggcctactg ggccgagccg
     2101 aacgagaacc caccgatccc tggtgcggcc gcgttgccgc ccaggccgaa gagcacgccc
     2161 gcgaacgagc tgacgggcgt gatcacattc agcgaggtgc tgatggcgga gccctgcgtt
     2221 acttgcgagg tcgagaggac ctgcaggccc gcgttttcgg cgctggccat cgccgtgttc
     2281 aggtcggtgg cgccgacgaa attacccgga ttcgccggat cctgcagaat gccgaacacc
     2341 aggctcaggc cgccggccat cacgccggag ctttggccgc ggacttccat cccctgcgaa
     2401 tcggacatcg tctgcatctg gccgaggccc agacgctgga gcttcgactg gggaatctgg
     2461 ccgtcgccgg ccagcgcggg gctagcagcc accgccaaca acagaaacac aggtgcgaac
     2521 ttcatggtgc aactcccaaa actagagtga tgtggaaaaa accgcgccag gatggcaacc
     2581 ctcgcgactg ccgaaatctg cacaaccttt gctgctgccc cgcgtgttac tttcgatgct
     2641 agttgtgcga cctgcgccct caatgatttc cgcaggtttc ttccgcgttt ttcttccgag
     2701 ccaggcgtta aagtcgcgct aaacgctatc tcctgcccaa cgtccctccc gcccgtgcca
     2761 gtcgtgccgg atgctccatg aattcgcaat agtcatgccg aatcgggtgt agggaatgtg
     2821 acggggatga cgataacgac ttgcaggcag cctctacggg ttgtcggcat ggtaagcatt
     2881 gcgcccgatc cagggccgcc gaagcgggtg tccgcattcc ggccaggcca cccatgccgc
     2941 gagtcgtcgt agcgaggcgg aaacaattcg gggggaaaca tgaggatcca gctccagatc
     3001 tgcgtctttg cggcctgcgc cctcgcggcg ctcggcgccg cgcggggaaa cgacgattcc
     3061 agtgaagcgt ttgcggtggc cgaccccgcc gaaacgctgc atgccgccag tccgatcggc
     3121 gcgagctcga tggacggtcc ggcagcatcg aactcgctca gtacgcagga cgctgaggcc
     3181 ttggcccagt gggccgagcg ccaggccgaa ctggccatca tcaacggcga atccgccgag
     3241 gccgtccagg agaagaagca gctcgccgtc tgggcgcggg cccaggccga tgaagccgct
     3301 agtccggcgg acatcgcgcc tgcggcgggt tttgccgcgg atcccctggc ctcgcccgtc
     3361 gtcttcaacc aggccgaagc actgcctggg caagcgccca tcctgccgcg gtttcgcgcg
     3421 ggccagccga ccttcgccag ccagccgccg cccggcccgc ccgctacggt cgatgccacg
     3481 ctgagcgacg acggacagga agtggtcccc acgcccacgc ctgtcgatgg cgtcattgtc
     3541 gatggctatg gttcgtggga cgacatctac gggatgtgca gtgtttgctg ctgctcggga
     3601 ttgttcctgg gcgtggaagg cacgttcctg gcgcccgtcg gcgagcccac caccggcgtt
     3661 ctgttgaccg atctggtctc cggcgctacc tacagcggaa cctcgcagcc cggactcggg
     3721 gccggcgtcc gcacctggct gggattgcaa aactgcaacg gcactggctt tgtcgtccgt
     3781 tactggacgt tcgggaatca ggtgatcaac cccgatcctg tcgtcccggt caacaaacag
     3841 ccggcctttc acaattccta ttttctgaac gcctatgcgt tggacatcga aatgatccag
     3901 cggttctgct ggcacggcac gcggtggtac gcgacgtttg gcggacgcta cgcccgcctg
     3961 gagcggaacc agctcgttgc cgggtacggg acggtcggcg atgtgagtct ctatggactg
     4021 gcccaggccg ccaacgaaat cgaaggcgcc ggctttacca cgtcgctgtg ggccacgggc
     4081 ccgctgtgcg ggtgctgcct cccctgcggc tttaactggt atggcggagc gcggatttcg
     4141 atcctctggg cggattcgct ggcgtcggct tacaccgaag ccaacgccgc ggtcaagccg
     4201 ccgatcggcg ccgctgtcgc caactcgatc gacgccgcat ttgccagcaa ggaccactcg
     4261 gaaacgattt tcatcgccga agctcaggct ggcattcagt acgagcactg cctgccgtgc
     4321 cacaatgcga tcttcttcgc ccgggccggc ttcgagtacc agcactggaa caccggcgat
     4381 gtctttgccg gcagcaattc cttcgcgttc ctggcgggcg ggccgccgcc gttcggcggc
     4441 cgcgtggatg ctttcgccaa cgcccacgac ggcaacctgg acttgattgg cttcgtcctg
     4501 ggcgcagggt tgacttacta acgagacgac cagcgtgtgg ccctctccgt gtgacttcct
     4561 gaatcgacat ccgggcaacc gcgcttccac ggcgaagcgc ggcaccggag gccggctggg
     4621 aacgaggcga atct
//
LOCUS       DASUGB010000259         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1003120, whole genome shotgun sequence.
ACCESSION   DASUGB010000259 DASUGB010000000
VERSION     DASUGB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003120"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFV87_05000"
     CDS             <1..201
                     /locus_tag="VFV87_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5103143.1"
                     /translation="LLPVWIMAYRYKDQVHRFLMNGQTGKSTGTAPISYHKIAAVVGI
                     VLAVILVGLVCMGIIAAIAGSH"
     gene            210..1067
                     /locus_tag="VFV87_05005"
     CDS             210..1067
                     /locus_tag="VFV87_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103144.1"
                     /translation="MFANVALAACLACGADSDVLFSESFDDAELAKRAWYDVGAVRIA
                     GDAAAGKACLEYEWVAGKDNVQGSSPMRRLFEPSDRVHVRFDLKLSEGFGWSGRSYHP
                     HLINLLTTENGKWHGPAASRLTLYIEPVGGKLRLAAQDIQNKDAPHGLTQGPLRGGYN
                     GKFYDSEQPVFLTPTRRASEGPTPTRSVSEGVTIEGWHRVEAYFQLNTLDLAADKPNR
                     DGIVRGWFDGKLVVEHLDIVFRTTDFPKMQFNQFFLGPYFGPGLLPHSQKLWIDELAV
                     ARERPANEQ"
     gene            1210..1629
                     /locus_tag="VFV87_05010"
     CDS             1210..1629
                     /locus_tag="VFV87_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEX5103145.1"
                     /translation="MATATFPQSRNTHSIAVGYLCWLLGFLGMHRFYYGKPITGTIWF
                     FTGGLLLIGWIIDLFLIPSMDAHADRRFVPGRLDYNIAWLLLVFPITGLLGLHRFYMG
                     KIVTGVIWLLTGSLFGLGWLYDLWTLNGQVSEINAQS"
     gene            complement(1732..3435)
                     /locus_tag="VFV87_05015"
     CDS             complement(1732..3435)
                     /locus_tag="VFV87_05015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103146.1"
                     /translation="MNDHAPQIDREPGGPLVGGALESGVQVGDFRIERRIGAGGMGIV
                     YQARQISLNRTVALKVLGQALTRSADVSRFQREAQAAARLKHPAIAQVYYIGQDRHLC
                     YLAMELIDGISLRRALDRLSTTQANDSSLDSIVKSDLVEQPSAASLRFDQPTEAATGD
                     NGQAVAADPKPAADVNPYLSPQARELRTSKIYVRRVCELIRDAARALAHAHEQGVIHR
                     DIKPDNLLLDRGGQVHVIDFGVARFFEDQAVTYTGQLVGTPLYMSPEQVTGRGAIDAR
                     TDVYSLGLVLYELLTLKPPIEATNRENLLRTIVTKPLAPISWRNKAVPPELERIIHRA
                     TQKDPDQRYATAAQLADDLDRFLDSKPVVAPPYHFRLDEREITAARPGGVVLAAFIFF
                     LVSFFVAIFFAATALMMGLMAMGSEGLVIGGTFAAFAAVVLGAGVTVGYGLLAAHQWA
                     RWTGVVLASLVTLLAATGVIGIIVGVVAVATNEDSIEKFFQGIEEQPKDSAGSETQTV
                     QPQNFVYALMGFYGLPIAVGLFLGGTALWALLNRATGAWFRFAAEIRAEHRLVRMRLA
                     E"
BASE COUNT          658 a         1217 c         1091 g          632 t
ORIGIN      
        1 ctgctgccgg tctggatcat ggcctaccgc tacaaggacc aggtccaccg cttcctgatg
       61 aacggccaga ccggcaaaag caccggcacc gcaccgattt cgtaccataa gatcgccgcg
      121 gtcgtcggca tcgtcctggc cgtgatcctg gtggggctcg tgtgcatggg catcatcgcg
      181 gccattgccg gaagccattg aggtgaatca tgttcgcaaa tgtggcgctc gccgcttgtc
      241 tggcctgcgg agccgattcg gatgtgctct tctccgaaag cttcgacgac gccgaactcg
      301 cgaagcgcgc gtggtatgac gtgggcgcag tccgcattgc aggcgacgcc gcagccggca
      361 aggcgtgcct ggaatacgaa tgggtggccg gcaaggacaa cgtgcaaggc tcctcgccca
      421 tgcggcgctt gtttgagccg agcgaccgcg tgcatgtccg cttcgacctg aagctctccg
      481 agggcttcgg ctggagcggg cgcagctatc acccgcacct gatcaatctc ctgacgaccg
      541 agaacggcaa atggcacggc ccggctgcca gccgcctgac actctacatc gagccggtcg
      601 gcggcaagct gcgcctcgcc gcgcaggaca tccagaacaa agacgccccg cacggcctca
      661 cgcaaggccc gctccgcggc ggctacaacg gcaagttcta cgacagcgag cagccggtgt
      721 tcctcacacc aacccgacgc gcaagcgagg gccccacacc aacccgaagc gtcagcgagg
      781 gagtcacgat cgaaggctgg caccgcgtcg aagcctactt ccagctcaac acgctcgacc
      841 tcgccgccga caaaccgaac cgcgacggca tcgtccgcgg ctggttcgac ggcaagctgg
      901 tcgtcgaaca cttggacatc gtcttccgca cgaccgactt ccccaaaatg cagttcaacc
      961 agttcttcct cggcccctac ttcggccccg gcctcttgcc gcactcgcag aagctgtgga
     1021 tcgacgaact ggccgtcgcg cgggagcggc cagcgaacga acaatgatcc ctgcaaggcc
     1081 gcccgattcg gccattctgg cattcgacgt gcagtacagc ttgtcggctg ccactttgac
     1141 gcgaaattca tgcgctaggc aacgcaagat cagcgccgca ttcgcgaata gccgaaaaag
     1201 gagatctgca tggccaccgc caccttcccg caatcccgca acacccactc gatcgccgtc
     1261 ggctacttgt gctggctgct gggatttttg gggatgcacc gcttctacta tggcaagccg
     1321 atcacgggga cgatctggtt cttcaccggc ggcctcttgc tgatcggctg gatcatcgac
     1381 ctgttcctga tcccgtcgat ggacgcccat gccgaccgcc gctttgtgcc gggccggctg
     1441 gattacaaca tcgcctggct gctcttggtg tttcccatca ccggcctgct cgggctccat
     1501 cgcttctaca tgggcaagat cgtcaccggc gtgatctggc tcctcaccgg cagcctcttc
     1561 ggcctcggct ggctctatga cctctggacc ctcaacggcc aggtcagcga gatcaacgcc
     1621 caatcgtagg cgacctcgct ccgcgagtcc gcgaaagggt cgagagtcaa ttgcggttca
     1681 gtcgatccat ttggatcgcg gatatcgcaa ttgactcccg accccgtgac gtcactccgc
     1741 cagccgcatc cgcacgaggc gatgctcggc tcgaatctcc gcggcaaagc ggaaccaggc
     1801 gccggttgct cgattgagca gcgcccacag ggcggttccg cccaagaaca ggcccacggc
     1861 gatcggcaat ccgtaaaagc ccatcagtgc atagacgaag ttctgtggct ggaccgtctg
     1921 cgtctcactt cccgcggagt ctttcggctg ctcctcgatg ccttggaaga atttctcaat
     1981 cgaatcttca ttcgtcgcga cagcgacgac gccaacaata atcccgatca cgcccgtcgc
     2041 agccagcagc gtcaccagac tggccagcac gacgccggtc cagcgagccc attgatgggc
     2101 ggcgagcagg ccgtagccca ccgtcacccc cgcgcccagg accaccgcgg cgaacgccgc
     2161 aaacgttccg ccgatcacca gtccttccga gcccatcgcc atcagaccca tcatcagcgc
     2221 ggtggccgcg aagaagatgg cgacaaagaa cgacacaaga aaaaagatga acgccgccag
     2281 caccacgcca cctggccggg ccgcggtgat ctcccgctcg tccagccgga agtgatacgg
     2341 cggggcgaca accggcttgg agtcgagaaa gcggtcgaga tcgtcggcga gctgagcggc
     2401 ggtcgcatac cgctggtccg ggtccttctg cgtcgcccgg tggatgattc gctccagttc
     2461 cggcggcacg gccttgttcc gccacgaaat tggcgccagc ggcttggtga cgatcgtccg
     2521 cagcaggttc tcgcggttgg tcgcctcgat cggcggtttc agcgtcagca gctcatagag
     2581 caccaggccc agggaataga cgtctgtccg ggcgtcgatc gccccgcggc cggtgacctg
     2641 ctcggggctc atatagagcg gcgtgccaac gagctggccg gtataagtca ccgcctggtc
     2701 ctcgaagaag cgggccacgc cgaaatcgat cacgtgtacc tgcccaccgc gatccaggag
     2761 cagattgtcg ggcttgatgt cgcggtggat caccccttgc tcgtgggcgt gagccagggc
     2821 ccgggccgcg tcgcgaatca gctcgcagac gcgccgcaca tatatcttgc tggtccgcag
     2881 ctcgcgggcc tgcggagaaa gatacggatt cacgtctgcg gctggcttgg gatccgcggc
     2941 gacagcctgg ccgttgtcgc cggtagccgc ttccgtcggc tggtcgaatc gcagggacgc
     3001 cgcggaaggc tgctcgacga ggtcgctctt cacgatcgaa tcgaggctcg aatcgttcgc
     3061 ctgcgtggtg ctcagccggt cgagcgcccg ccgcagcgag atgccgtcga tcagttccat
     3121 tgccagatag cacaggtgcc ggtcctggcc gatgtaatag acctgggcga tcgccggatg
     3181 cttcagccgg gccgccgcct gcgcttcgcg ctgaaagcgg ctgacgtcgg cggagcgcgt
     3241 gagggcctgc ccgagcacct tgagcgccac cgtccgattg agcgaaatct gccgggcctg
     3301 atagacaatg cccatgcccc ccgcgccgat ccgccgctcg atgcggaaat cgccgacctg
     3361 cacgccgctt tcgagcgcac cgcccacgag cgggccgcct ggctcgcggt cgatctgcgg
     3421 cgcgtgatcg ttcatgcaga cgattattgg ctggcgactc tcgggacgcg agtaggagcg
     3481 tagaattagc ccatgcgata ctacctgctc gccttgagtt gggtttccct ccttggcggc
     3541 tgtggcggca atgacgccaa cgaggtcatc gtctatgcgg ctctcgaccg cgaattct
//
LOCUS       DASUGB010000260         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1559271, whole genome shotgun sequence.
ACCESSION   DASUGB010000260 DASUGB010000000
VERSION     DASUGB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559271"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2782
                     /locus_tag="VFV87_05020"
     CDS             <1..2782
                     /locus_tag="VFV87_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103147.1"
                     /translation="EAVGRFAWNAPLWRSVRLSPEGVYLVGPDNARDLAATREHGHLF
                     VWKRDEPKPTGRLTVPGPVIWMDFVAAEEVAALTFGPEAVLQIWKVATGELTKSIELP
                     KNEFSLPTRDPLDPNGRADPNRKAYLPNPWAGAVSPGGRYVFLGGTNGIVTLSLAEGK
                     TVGTLPLPSSFKAVARVGGTPPYAGRGEFRGLGFSADARKLWALVWNEQANVVTWSLE
                     TGRPLGAVVVGRPDVAWRYCGAPLAGSEPNTLLVPQQEFASFLTVPGLGGELKTGSLI
                     DARTGAVLGDLEVLPVRWAGEGLLLALGDPANAKHLLPASVTGGRKAAIVVAAEKLIL
                     KRPPLPPNFRVAGVIDEVNAPPPQPRPDLQPADRAGLSPSTATPPTAWIAPPVPPAVA
                     ERLVGARHVPKTRPLIGGTHAAFLHSWPTLDGRVNGKPTLAQGIHWHRYDLRTGQATG
                     PAVTLWPWATSPADGKSMKLEEEMVAAALSPDGERLALRDPADLARVDVWDAAGQRLL
                     GMQPFGANTPIQWVGWSQSGRLLTLGDGKLTAWDVPAGKAVYEVAGGYRLPVAETRGR
                     AWLAVTGEGHVDLVEADSGRCLGRVPLAGAELPDDLAALSISPDGKLLAYCGRRTDSS
                     PAQLQPEMAGWLLTWDLATGKPHPPLEVLRLPGWEIVAFDSVRVLVGGALFDLRCGTS
                     VAAYNCRFLADSADGRLWAIGIDPDDEGQRAQPIFDRAGRPSDPARQPKLALRAVELV
                     AYDTGPTSPADLFTGATPITVEADLQNLEKSRELGQSEVALLQKNGFTVGKSQWRLRG
                     DAEIFDSGSRFTNGVHLPAIRVHWRLTSPDGLEAWKNSRLLLWDEVLGKYGVSSEVVK
                     KKGPLGEEEYILEKFDFQGRSPRQTIIDDLLEKSGHYSTIEGLPKLMGKFNGRYQSLP
                     VLTEVRYLPMNP"
     gene            complement(3009..>3311)
                     /locus_tag="VFV87_05025"
     CDS             complement(3009..>3311)
                     /locus_tag="VFV87_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103148.1"
                     /translation="SDLYLRYVIGQLDPELLHTSRERHIRCNPTLAQFIVDPDFEPVV
                     VSGDFDKRQFDPAFVREQERLLTRGWKRLKELAHIEGLSLLDYPLPEVREKMRERT"
BASE COUNT          551 a         1040 c         1142 g          578 t
ORIGIN      
        1 cgaggcagtg gggagattcg cctggaatgc gccgctctgg cggtccgtgc ggctcagccc
       61 ggagggcgtc tatcttgtcg gaccggacaa tgccagggac ttggcggcca cgcgcgagca
      121 cgggcacttg ttcgtctgga aacgggatga gccgaagccg accggccgcc tgactgttcc
      181 gggacctgtg atctggatgg atttcgttgc cgctgaagag gtcgcggcgc tcacattcgg
      241 tccggaagcg gttctgcaga tctggaaggt ggccacgggc gagctgacaa agtcgatcga
      301 gctgcccaag aacgaattct cgctgcccac gcgcgaccca ctcgatccga atggcagagc
      361 cgatccgaac cggaaggcct acctgcccaa tccgtgggcc ggcgcggtca gccccggcgg
      421 ccgctacgta ttcctcggcg gcacaaacgg catcgtcact ctctcactcg ctgagggcaa
      481 aacagtcggc acgctgccgc tgcccagcag cttcaaggcg gtcgcacgcg ttggcggcac
      541 acccccgtac gccgggcggg gcgagtttcg cggcttgggg ttcagtgctg atgcccgcaa
      601 gctgtgggcg ctggtatgga atgaacaggc aaacgtcgtg acgtggtcgc tcgagaccgg
      661 gcggccgctc ggcgcggtgg ttgtcggccg gcccgacgtt gcctggcgat attgcggtgc
      721 accattggct ggctccgagc cgaatacact gctcgtgccc cagcaggagt ttgcgagctt
      781 tctgaccgtg ccgggacttg gcggcgagct gaagacgggg tcgctaattg acgctcggac
      841 cggggccgtg ctgggcgacc tggaggtgct gcccgtccgc tgggcgggcg aagggctgct
      901 gctagcgctg ggcgatccgg caaatgccaa gcacctgctg ccggcgtcgg tgaccggggg
      961 gcgcaaggcg gcgatcgtgg ttgcggccga gaagctaatt cttaaacgac cgccgttgcc
     1021 gcccaatttt cgcgtggcgg gcgtgattga tgaagtcaac gctccgccgc cccagccgcg
     1081 gcccgatttg cagccggctg accgcgcggg cctttcccca agtaccgcca cgccgccaac
     1141 ggcttggata gcgccgcccg tgccaccagc cgtcgcagaa agactcgtgg gtgcccgcca
     1201 cgtgccgaag acccgcccgc tgatcggcgg cacgcacgcc gccttcctgc acagttggcc
     1261 gaccctcgat ggccgcgtga acggcaagcc aacgttggct cagggaattc attggcaccg
     1321 ctatgacctg cgcaccggcc aggccaccgg tccggccgtg acgctctggc cgtgggccac
     1381 ttcgcccgcc gacggcaaga gcatgaagct ggaggaggag atggtcgccg cggctctgtc
     1441 gcccgatggc gagcgcctgg cgctgcgcga cccggccgac ctggcgcgcg tcgatgtgtg
     1501 ggatgccgcg ggccagcggc tgctgggcat gcagcccttt ggcgcgaaca cgccgattca
     1561 gtgggtcggc tggtcgcagt cgggccggct gctcacgctc ggcgatggca agctgaccgc
     1621 ctgggatgtg ccagccggaa aggcggtgta cgaagtcgcg ggcggttacc ggctaccggt
     1681 ggccgaaacg cgcgggcggg cgtggctggc cgttaccggc gagggtcacg tcgatctggt
     1741 cgaagccgac agcggccgtt gcctggggcg cgtgccgctc gccggcgcgg aattgcccga
     1801 cgatttggcc gcgctgtcga tctcgcccga tggcaagctg ctggcatatt gcggtcgacg
     1861 gaccgacagc agccccgcgc agctccagcc cgaaatggcc ggctggctgc tcacctggga
     1921 tctggccaca ggaaagccgc acccgccgct cgaagtcctc cgcctgcccg gctgggaaat
     1981 cgtcgccttc gattccgttc gcgtgctggt cggcggggcg ctgttcgatc tgcgctgcgg
     2041 cacctcggtc gccgcttaca actgccgctt cctggccgat tcggcggacg gccgcctgtg
     2101 ggcgatcggc atcgaccccg acgacgaggg ccagcgcgcc cagccaatct tcgatagagc
     2161 gggacggccg agcgatcccg cccgtcagcc gaagctcgcc ctgcgggccg tcgagctggt
     2221 ggcttatgat acgggcccga ccagcccggc cgacctgttc acgggcgcga cgccaatcac
     2281 ggtcgaagcc gacctgcaga atctggaaaa gagccgggaa cttggccagt cggaggtcgc
     2341 cttgttgcaa aagaacggct tcacggtcgg caagagccag tggcgactgc gcggcgatgc
     2401 ggaaatcttc gacagcggca gccgcttcac gaatggcgtg catctgccgg cgattcgggt
     2461 gcattggcgg ctcacgtcgc ccgacggtct ggaggcgtgg aaaaattcgc ggcttctgct
     2521 gtgggacgag gtgctgggca aatacggcgt ctcatccgag gtggtcaaga agaaggggcc
     2581 gctgggggag gaggaataca tcctggagaa gttcgacttc caggggcgca gtccgcgcca
     2641 gacgatcatc gatgacctgc tcgagaagtc cggccactat tccaccattg aagggctccc
     2701 caagctcatg ggcaagttca acggccgtta ccaaagcctg ccggtgctga ccgaagtgcg
     2761 ctatctgcca atgaatccct gatggcgggc ttgcagaatt ccggcagcga gctggcgaag
     2821 agccacgacc cccaattctc tatgctggat ttgtttgttg atgcaatgtc ctcttgcccc
     2881 cgtgctgagc ctcttttgac gagcactggg tcatcattgg atttggtgac cgcggatatt
     2941 gggaggattt ttacgaactc aaccgcaagt ccgcccgagt aaccgaggat tgaaacagtg
     3001 ttttcatact atgtgcgctc tcgcatcttc tcgcgtactt ccggcagcgg gtaatcgagg
     3061 agcgaaaggc cctcaatgtg ggccagttcc ttgaggcgct tccagccgcg ggtgagcagg
     3121 cgttcttgtt cgcggacgaa tgccgggtcg aactggcgct tgtcgaagtc gccgctgacg
     3181 acgaccggct caaagtcggg atcgacgatg aactgggcca gcgtggggtt gcagcggatg
     3241 tggcgctcgc ggctcgtatg cagcagttcg ggatcgagct ggccgatcac gtaccgcagg
     3301 tacagatcgc t
//
LOCUS       DASUGB010000261         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_724731, whole genome shotgun sequence.
ACCESSION   DASUGB010000261 DASUGB010000000
VERSION     DASUGB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724731"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFV87_05030"
     CDS             <1..1502
                     /locus_tag="VFV87_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5103149.1"
                     /translation="TEQLAGDLLPNPTLDQQVASGFNRCHVTTSEGGSIEEEVYCRNV
                     FDRTETFAQVFLALTFTCARCHDHKFDPVTQREFYQISAAIGGTYQGDERETVSGKGR
                     AAADKRIAKLRSEIQTLEGKPELAAQRSRLQSVVRLLEAGKVHTTAPRQPDAWRILAR
                     GDYRQPGDVVSPRGIAAITGVSPDWGLAEDAPEAERRKALANWIADAKNPLTPRVVVN
                     RLWAHHFGEGLVRTPSDLGFQGGQPSHPELLDWLAGQLLQPNDGKPWSLKHIQRLIVN
                     SATYRQESRILDFGLPILDSNEPEEPVNPKSKIQNPKSIDADNRLLWRMNRRRLEAEV
                     FRDAVLAASGELDLRIGGPGYKDYKVTSAGNNETYTVFDAVGKEFNRRSLYRTSVRSG
                     TSPLLDTLDCPDPSVAAPRRSVTTTPLQALTLLNNVFTEHYAARFAERLKREAPAEEA
                     AQIQRAYALAFGRPANEDELKFALQFIGQHGLDQFCVVIFNASEFLYVD"
     gene            1492..2958
                     /locus_tag="VFV87_05035"
     CDS             1492..2958
                     /locus_tag="VFV87_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103150.1"
                     /translation="MSIDPLPNPQSAIRNPQFNRRDLLSQFATGLSGMALASLLARDS
                     RGATVPAEAADPPPHHAPKAKRAIQIFLQGGLSQVDSFDYKPELNKLHGQPVPGDEKP
                     QAFMGKVGLLHKAHFKFKQRGESGLWVSDLFPHLADIADELTVIRSMWSGTGNHTPAT
                     YEANSGFRTLGFPAAGTWISYGLGCEVNNLPTFVVLPDSRSLPTGGANNWSSGFLPAR
                     HQGVMLNTRGPAVRDLTPSSKTDSQTQAARFAAVAELNRRHLAERAGDDALEGRIKSY
                     ELAARMQLAIPEAIDLAQESRQTLDMYGVEGKDSGDFARACLMARRLVERGVRFVQLW
                     SGAAFGTEVHWDAHGSVPDNHRRESAKIDQPVAALLRDLRQRGMLDDTLVILNTEFGR
                     TPYAESAGDKAGAGRDHNADAFSVWLAGAGLKHGLAYGTSDDIGWKAADHPVDVHDFH
                     ATILHLLGIDHTRLTFYHNGIRRRLTNVHGRVLTDLLA"
     gene            complement(2999..3616)
                     /gene="coaE"
                     /locus_tag="VFV87_05040"
     CDS             complement(2999..3616)
                     /gene="coaE"
                     /locus_tag="VFV87_05040"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5103151.1"
                     /translation="MLTLGLVGGIASGKSVVADCFRDLGAVVLDADRAGHAVLREPDV
                     IAALKARWGERILDASGQVSRREVAKIVFAREDDSERQFLEQLTHPRIQMRLQQELAK
                     AQAAEEPPRVVVLDAALLFEAGWERLCDKIIFVDAPRDVRLERAVARGWSAEQFAARE
                     AAQLPALEKRRRADLVIRNVRTLENIRDVVRLTWNDLLGGKPRAG"
BASE COUNT          689 a         1287 c         1177 g          604 t
ORIGIN      
        1 tgaccgaaca gcttgcgggc gacctgctcc cgaacccgac gctcgaccag caggtggcca
       61 gcggcttcaa ccgctgccac gtgacgacga gcgagggcgg gtcgatcgag gaggaggtct
      121 actgccggaa cgtcttcgac cggaccgaga cgttcgctca ggtcttcctc gcgctcacct
      181 tcacctgcgc ccgctgccac gaccacaagt tcgatcccgt cacgcagcgc gagttctatc
      241 agatatcggc cgccattggc ggcacgtacc agggagacga gcgcgaaact gtttcgggca
      301 aagggcgggc cgccgcggac aagcggattg cgaagctgcg atcggaaatt caaacgctgg
      361 aaggcaagcc cgaactggcc gcacagcgct cgcgcctgca aagcgtcgtg cgattgctgg
      421 aagcggggaa agttcacacg accgccccca ggcagcccga tgcctggcgg attctcgccc
      481 gcggcgatta ccgccagccg ggcgacgtgg tttcgccgcg cggcattgcg gccatcacgg
      541 gcgtctcgcc ggactggggt ctggctgaag acgcgcccga agcggagcgc cgcaaggccc
      601 tggccaattg gattgccgat gcgaaaaacc cgctcacgcc gcgggtggtt gtaaaccggc
      661 tttgggcgca ccatttcggc gaaggactgg tgcggacgcc cagcgacctg ggcttccagg
      721 gcggccagcc gtcgcatccg gagctgctcg attggctcgc gggacagctc ctgcagccta
      781 acgacggtaa gccgtggagc ctgaaacaca tccagcgctt gattgtgaac tcggcgacgt
      841 accggcagga atcgcggatt ttggattttg gattgccgat tttggattcc aatgaaccag
      901 aggagcctgt gaatccaaaa tccaaaatcc aaaatccaaa atcgatcgac gccgacaacc
      961 gcctcttgtg gcgcatgaac cgccgccgcc tggaagcgga ggtcttccgc gacgcggtcc
     1021 tcgccgcgag cggcgaactg gacctgcgca tcggcggtcc cggctacaaa gattacaagg
     1081 tgactagcgc cggcaacaac gagacctata ccgtcttcga tgctgtcggc aaggagttca
     1141 atcgccgcag cctgtatcgc acgtccgtcc gctcgggcac gagcccgctg ctggatacgc
     1201 tcgattgccc cgatccgtcg gtcgccgcgc cccggcgctc ggtcacgacc acgccgctgc
     1261 aggccctgac gctgctcaac aacgtcttca ccgagcacta cgcggcccgc tttgccgagc
     1321 ggctgaagcg cgaagcgccg gccgaggaag ccgcgcagat tcagcgggcg tacgcgctgg
     1381 cctttggacg tccggcgaat gaagacgagc tgaagtttgc gctgcaattc atcggccagc
     1441 acggcctgga ccagttctgc gtggtgatct tcaacgccag cgagttcctg tatgtcgatt
     1501 gatccgcttc ccaatccgca atccgcaatc cgcaatccgc aattcaaccg gcgcgacctt
     1561 ctcagccagt tcgccacggg cctctccggc atggccctgg caagcctgct ggcccgcgac
     1621 tcgcgcggcg cgaccgtccc cgccgaggcc gccgatccgc cgccgcatca tgcgccgaag
     1681 gcgaagcggg ccattcagat cttcctgcaa ggcggcctga gccaggtcga ttcgttcgac
     1741 tacaagcccg agctcaataa gctgcacggg caaccggtcc ccggcgacga gaagccgcaa
     1801 gccttcatgg gcaaggtggg gctgctccac aaggctcatt tcaagttcaa gcagcggggc
     1861 gaaagcggcc tgtgggtctc ggacctcttt ccgcacctgg ccgacatcgc cgatgaattg
     1921 accgtgattc gctcgatgtg gtctggaacc ggcaatcaca caccggcaac ctacgaggcc
     1981 aacagcggct ttcggacgct cggctttccg gcggcgggga cctggatttc ctacggcctg
     2041 gggtgcgagg tcaataactt gcccacgttt gtcgtcctgc ccgacagccg ctcgctgccg
     2101 actggcgggg cgaacaactg gtcgagcgga tttttgccgg cgcggcatca gggggtgatg
     2161 ctgaacacgc gcgggcccgc cgtgcgcgac ctgacgccgt cgtcgaagac ggattcccaa
     2221 acgcaggccg cccgtttcgc cgcggtggcg gaattgaatc gccgtcatct ggccgagcgg
     2281 gccggcgacg acgccctcga aggccggatc aagagctacg agctggctgc ccggatgcaa
     2341 ctggcgattc ccgaggcgat cgatctggcc caggaatcgc gccagacgct ggacatgtac
     2401 ggcgtggagg gcaaggacag cggcgatttc gcccgcgcct gcctgatggc ccgccggctg
     2461 gtcgagcggg gcgtgcggtt cgtgcagctc tggagcggcg cggccttcgg caccgaggtc
     2521 cactgggacg ctcacggcag cgtgcctgat aaccaccgcc gcgaatcggc caagatcgat
     2581 cagccggtcg ctgcgctact gcgcgatttg cggcagcgcg gcatgctgga cgatacgctc
     2641 gtgatcttaa acaccgaatt cggccgcaca ccctatgccg aaagcgccgg cgacaaggcc
     2701 ggcgccggcc gcgaccacaa tgccgacgcc ttcagcgtct ggctcgcggg cgccggcctc
     2761 aagcacggcc tcgcctacgg cacgtccgac gacatcggct ggaaggccgc cgaccacccc
     2821 gtcgacgtcc acgatttcca cgccacaatc ctgcatctcc tgggcatcga ccacacgcgc
     2881 ctcacgttct atcacaacgg catccgccgc cggctgacca atgtccatgg ccgcgtgctg
     2941 accgatttac tggcttgata caggcaacac caacccgacg cgcaagcgag ggcgtccgtt
     3001 aaccagcccg cggctttccg ccgagcagat cgttccacgt cagccgcacg acgtcgcgga
     3061 tgttctccag cgtgcggaca ttgcggatca ccaggtccgc ccggcggcgc ttctccaggg
     3121 ccggcagctg ggccgcttcg cgggcggcga attgctcggc cgaccagccg cgggccacgg
     3181 cccgctccag gcggacatcg cgcggagcat cgacgaagat gatcttgtcg caaagccttt
     3241 cccagccggc ctcgaagagc aaggcggcgt cgagcacgac gacccgcggc ggctcctcgg
     3301 cggcctgggc tttggcaagc tcctgctgca agcgcatctg aatccgcgga tgcgtgagct
     3361 gctccaaaaa ctgccgctcg ctgtcgtctt cgcgggcgaa gacgatcttg gcgacctcgc
     3421 ggcgcgagac ctggccggaa gcatccagaa tccgctcgcc ccagcgggcc ttgagtgcgg
     3481 caatcacgtc cggctcgcgc agcaccgcat gcccggcccg gtcggcatcg agcacgaccg
     3541 cccccaggtc gcgaaagcaa tccgcgacca cgctcttgcc gctcgcaatg ccgccgacca
     3601 gtcccagggt gagcatgatg gcgcagttca attccaatga agaaggctcc attattcaca
     3661 tcgagtggcg caacgccaca cgccctgtgt tttcgcagcg aaaacgcact cgtgcgcttg
     3721 tcgctggccc tccgatctgg cgataatcca agcagac
//
LOCUS       DASUGB010000262         6897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_864308, whole genome shotgun sequence.
ACCESSION   DASUGB010000262 DASUGB010000000
VERSION     DASUGB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864308"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..809
                     /locus_tag="VFV87_05045"
     CDS             315..809
                     /locus_tag="VFV87_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5103152.1"
                     /translation="MNQADLRSILQALPFSAGLSDATAEKLAGLVSLQQFSGGSVIFA
                     EGAANPSVYLIVEGEVALEMCVPARGCTRILTLGPGELLAWSSLLGGGRMTAGALALT
                     DVRMLVAPAKAIGDLCAADRDVGFEFFRSVAVALSKRLVATRLQLLDLYGDAAPQPTA
                     AGIS"
     gene            831..1922
                     /locus_tag="VFV87_05050"
     CDS             831..1922
                     /locus_tag="VFV87_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5103153.1"
                     /translation="MEQFFETRGLQALIDVLKGNGYTVVGPTIQQAAIVYDRIEKVDD
                     LPRGWTDDQKPGQYRLHRRDDEASFGYVVGPHSWKKYLFPPITTLLRSQRTAEGWSFE
                     APVDEAPPFALLGVRACELAALAIQDKVFTGGCYVDPVYAERRRRLFIVAVNCTQAAE
                     TCFCVSMKTGPRCHSGFDLALTELATGFLVEAGSDAGRNLLSAIPCRAATPAEIQEGE
                     TRRGRATQQSRKMETSDIHDRLLANLQHPHWDNVAERCLSCANCTLVCPTCFCSSVED
                     VSDLHGDNVERQRRWDSCFNLDFSHIHGGPVRPTIHSRYRQWLTHKLATWYDQFGSSG
                     CVGCGRCITWCPVGIDLTEEVAAIRGTGS"
     gene            1919..2806
                     /locus_tag="VFV87_05055"
     CDS             1919..2806
                     /locus_tag="VFV87_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX5103154.1"
                     /translation="MSDSKIVAPAEARHPWVPMTAVIETLQRETPGVATYALQLTDAT
                     QAANYSFQPGQFNMLYLPGHGEVAISLSGRADPGNSGFNRPDRLIHTIREAGSVTQAI
                     ARLGVGGRLALRGPFGKPWPSESMKGCDIVLVGGGIGLAPLRPLLYTLLDQRGSFGRC
                     VLLYGARSADHLLYAADYHAWAATGLEIHTTVDRPSAGWNGNVGVVPLLIDRLRLGSA
                     RNTLFLMCGPEVMMRFSARAALERGIAADRIWVSLERHMQCAVGLCGHCQLGTELLCR
                     DGPVFRWDRVEPLLHIHDL"
     gene            2836..3636
                     /locus_tag="VFV87_05060"
     CDS             2836..3636
                     /locus_tag="VFV87_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5103155.1"
                     /translation="MFKFASCDGCQLSLLDCEDELLAIADAVEIAHFAEASSRLLDGP
                     YDVTLVEGSIATPHDAQRIREIRRQSRKLITIGACATAGGIQALRNFADHADFLRAVY
                     ASPEYIQSLDTSTAIADHVPVDFELRGCPIDRHQLLEVMTSLLAGRRPKTSGESVCPG
                     CKRRGTVCVIVAQNIPCLGPVTQAGCGALCPAYDRGCYGCFGPARQVNLGSLADHLLA
                     NGTQPTALIHLLRNFNGYAPAFRAASQQLEAQAATTDLDSGDASRVGA"
     gene            3623..4924
                     /locus_tag="VFV87_05065"
     CDS             3623..4924
                     /locus_tag="VFV87_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX5103156.1"
                     /translation="MSAHSTRTIRVEALTRVEGEGGLNVRLQDGQVAAVELNIYEPPR
                     LFESLLRGRALEEAPDITARICGICPVAYQMSAVHALEAALGVQTSPEIRRLRRLLYC
                     GEWIESHSLHIHLLHAPDFFGCDSVVELAAKFPDEVRRGLRLKKHGNELLEVLGGRAI
                     HPINVAVGGFYRTPRREELDRLVPSFEWGLEAAIAATRWVATFDFPAAQRDYEMVALH
                     HPDEYALNEGRIACTSGLDIPVEEYEAHFEERQVPHSTALHSVRLNSDRPYLVGPLAR
                     LAISRQQLGAYARQLADELGFPTPCRNPYLSIVARGLEVVHAYEEALQILRTYVPPAI
                     SRIPYSPRAASGCAATEAPRGLLYHRYEINREGKLVLAKIVPPTSQNQWQIEDDLLAL
                     LPDFLSRGDQEVAINCERAIRNYDPCISCSTHFLKLKIDGC"
     gene            4926..5381
                     /locus_tag="VFV87_05070"
     CDS             4926..5381
                     /locus_tag="VFV87_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX5103157.1"
                     /translation="MQTTCIVGLGSTHGDDQFGWLAADRLALEIELHHIADVTVRRAT
                     APAQLLDWLTGIDQLLLIDACRQPGRCGELQQLNWPAPEVEELTGSGSHSFSVWQTLT
                     LASELGTLPPTCRIWCAAGERFEADQDLSPSLLALLPRVVDDVLRWLAQ"
     gene            5391..5753
                     /locus_tag="VFV87_05075"
     CDS             5391..5753
                     /locus_tag="VFV87_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX5103158.1"
                     /translation="MHESSLVRSLLERVREVCEPAPLAEVEEVVVAIGPLAGVEPLLV
                     ASAFELQTRDTGLEQTRLRIEQVPLRLTCVSCGAAWERDEIEFACPACNCNQTRVVSG
                     EGMIIRHIVLGDPERVMK"
     gene            5750..6418
                     /gene="hypB"
                     /locus_tag="VFV87_05080"
     CDS             5750..6418
                     /gene="hypB"
                     /locus_tag="VFV87_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX5103159.1"
                     /translation="MSRVIVVERDVQSSARASAEEFRRQRTSAGTLVVNLVSSPGAGK
                     TSLLQATARRWTGLYRLAVLVGDLATDRDAQRLAPLVEVVQMTTGGACHLELPLVERA
                     FGRLASPELDFLFIENVGNLVCPASHDLGEHLRVCLLSTPEGDDKPGKYPKMFRGSGA
                     LIISKTDLLPHVPFSLDAAADDARQIRPGLPVFPVSSLNGSGLAAWCHFLEEQRKVRV
                     PKVR"
     gene            6415..>6897
                     /locus_tag="VFV87_05085"
     CDS             6415..>6897
                     /locus_tag="VFV87_05085"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5103160.1"
                     /translation="MSTSAVVKREAGVVDRILAVQLSVAGCVQGVGLRPTIARLAAQL
                     DLAGHVRNTPAGVVLVLEGSPAAIDAFVPALGAQLPPEARVRSIERTEIASRGRSGFQ
                     ICAASVEGAVSAPIPLDMATCPRCLADIAEPGNRRYGYAFTSCTDCGPRYSIIRELPF
                     E"
BASE COUNT         1221 a         2082 c         2160 g         1434 t
ORIGIN      
        1 gaccgcttcc tgcttgaact cctccgtgta cgaacgccgc gtccggacct ttcgcggcag
       61 ctttgcctgt gccattggat cgtccttcct cttccttgca gaataaggag ttggactgtc
      121 cacaaaaacc caagcacctc aatctctctc ttccacggct ctttgacctc gagactgagt
      181 tcccgagatg gatgcgagat ttcgtggggc cggaggcggg caagttgggc cacgcgtctg
      241 gaatgcacca tgccaatcct acagaatgat ttggtgtcac acattcctcg aatggccagg
      301 caaggatcgt ccctatgaac caggcggact tgcgaagtat cctgcaggcg ctgccattca
      361 gtgccgggct tagcgacgct acagccgaga agctggctgg cctagtttcc ctccaacagt
      421 tttcgggcgg ttccgtgatc tttgcggaag gggccgcgaa tccatcggtg tatctgatcg
      481 tcgaaggtga ggtcgcgcta gagatgtgtg tgccggctcg ggggtgtacc cggattttga
      541 ccttggggcc gggcgagttg ctcgcctggt cctcgctttt gggcggtgga cgaatgacgg
      601 ccggcgcgct tgcactaact gacgtgcgaa tgctcgtagc accggcgaag gcaatcggcg
      661 atttgtgtgc tgctgatcgt gacgttggtt tcgagttctt tcgaagtgtt gccgtcgccc
      721 tttcgaaacg gcttgttgcg acgcgactgc aactcctcga cttgtacggc gacgctgctc
      781 cgcagcccac cgcagccggc atctcgtaat cgagggaatg gtttggaatc atggaacagt
      841 ttttcgaaac gcgcggcttg caggcattga tcgatgtcct gaaagggaac gggtatacag
      901 tcgtcggccc gaccatccag caggccgcca tcgtttatga ccggattgag aaggtcgatg
      961 acttgccgcg cgggtggacc gacgatcaga agccggggca gtatcggctg catcgccgcg
     1021 atgacgaggc ctcgtttggc tacgtcgtcg gcccgcattc gtggaagaag tacttgtttc
     1081 caccgatcac gacactactg cgatcgcagc ggacggcaga gggctggtcc ttcgaggcac
     1141 ccgttgatga ggcgcctcct ttcgcacttt tgggcgtgcg cgcatgcgaa ctggcggcct
     1201 tggccattca ggacaaggtg ttcaccggcg gatgctacgt tgatccagtt tacgccgagc
     1261 gccggcgacg attgttcatc gtggctgtca attgcacgca ggctgcagaa acctgctttt
     1321 gtgtgtcgat gaagacgggg cctcgctgtc attcgggatt cgatctcgca ctgacggaat
     1381 tggcgaccgg atttcttgtc gaggcgggga gtgatgccgg ccgcaatctg ctcagcgcaa
     1441 ttccttgccg agcagcaact ccggcggaaa tccaggaagg cgaaacgcgg cgcggccgcg
     1501 ccacgcagca gagccggaaa atggagacca gcgacatcca cgaccggttg ctggcgaact
     1561 tgcagcaccc tcactgggac aatgtcgcag agcgatgcct ttcctgcgcg aactgcacac
     1621 tggtttgccc tacctgcttt tgcagcagcg tggaagatgt tagcgatctg cacggcgaca
     1681 acgtcgagcg ccagcggcgg tgggactcgt gcttcaatct cgacttcagc catatccatg
     1741 gcggcccagt acgcccgacg attcattccc gctatcgtca atggctgact cacaaactgg
     1801 caacgtggta cgaccagttc ggcagctcag gttgcgtcgg gtgcggtcgt tgcatcacct
     1861 ggtgtcccgt aggcatcgac ctgacggagg aggtcgccgc gatacgaggg accggatcat
     1921 gagcgattcc aagatcgtgg ccccggctga agcccgccac ccctgggtcc cgatgacagc
     1981 cgtcattgaa accctgcaac gagaaacgcc aggcgtcgcg acctatgcat tgcagctgac
     2041 agacgcaacg caagcggcga actactcgtt tcaaccgggc cagttcaaca tgctgtattt
     2101 gccgggtcat ggagaagtcg ccatcagcct tagcggtcgc gccgatcctg ggaattcagg
     2161 attcaatcgc ccggaccgcc tcattcacac gatccgcgaa gcgggctccg ttacccaagc
     2221 gatcgctcgc cttggcgtgg gcggcaggct ggcgctgcgc gggcccttcg gcaagccgtg
     2281 gccctcggaa tccatgaagg ggtgcgatat cgtgctcgtc ggaggtggca ttgggctggc
     2341 gccgcttcga ccgctccttt atacacttct cgaccagcga ggttccttcg gccgctgcgt
     2401 gctgctctat ggcgcgaggt cggcagacca tctgctctat gctgcggatt atcacgcttg
     2461 ggccgcaacc gggctggaga ttcacacgac ggtggatcgg ccttccgccg gctggaatgg
     2521 caacgtcggt gttgtgccgc tattaatcga tcggttgcga ttgggcagcg cccgcaacac
     2581 cctgtttttg atgtgtgggc cggaggtgat gatgcgcttc tcggcccgtg ccgctctgga
     2641 gcgcggcatc gcggctgacc ggatctgggt ctcgctcgag cgccacatgc agtgcgctgt
     2701 cggcttgtgt ggccattgcc agctcggcac tgagctcctc tgccgcgatg ggccggtctt
     2761 ccgctgggac cgggtcgaac cattgcttca catccacgat ctatgacaac tgccgccact
     2821 aaaattcgac tggccgtgtt caagttcgcc tcgtgcgacg gttgtcagct ttcgctcctc
     2881 gattgtgaag atgaattact cgccatcgcg gacgctgtgg agatcgcgca ctttgctgaa
     2941 gcttcgagcc ggctgctgga tggcccttac gatgtgacgc tcgtcgaagg gtcgattgcc
     3001 actccgcacg acgcccagcg aatccgcgaa atccgccggc agtcgcgaaa gctgatcacg
     3061 atcggcgctt gtgccacagc aggcggcatt caggccttgc ggaactttgc cgaccacgcc
     3121 gatttcctgc gggccgtcta tgcttcgccg gaatacatcc aatcgctcga cacttcgacg
     3181 gcgatcgccg atcacgtgcc ggtcgatttc gagctgcgag gttgcccgat cgatcgccat
     3241 cagctcctgg aagtcatgac ctcactcctg gcgggccgcc gtcccaagac ctccggcgag
     3301 tcagtctgcc ccggctgcaa gcggcgggga accgtgtgcg taatcgtcgc ccagaacatt
     3361 ccctgcttag gacccgtcac gcaagccggc tgcggtgcgc tgtgccctgc ctatgaccgc
     3421 ggctgctatg gatgcttcgg accggcgcgg caggtcaatc tcggcagtct tgccgatcac
     3481 ctgcttgcta acgggacgca gccgacggcg ctcattcact tgctgcggaa tttcaatggg
     3541 tacgccccgg catttcgcgc ggcaagtcag cagcttgagg cccaagctgc gacaacagat
     3601 cttgattcag gagacgcatc gcgtgtcggc gcatagcacc cggaccattc gcgtcgaagc
     3661 gctgactcgc gtcgaaggtg agggcgggct caacgtgcgc ctgcaggacg gacaggttgc
     3721 ggccgtcgag ctcaacatct atgagccgcc gcggctgttc gaatcgcttc tccgcggcag
     3781 agcgctggaa gaggcgccgg acatcaccgc gcggatttgc ggcatctgcc cggtcgctta
     3841 tcagatgagc gcggtccacg ccttggaagc cgccctgggc gtgcagacgt cgccagaaat
     3901 tcgccgcctg cgacggttgc tttattgcgg cgaatggatc gagagccatt cgctgcatat
     3961 tcacctgctc cacgcgcccg atttcttcgg ctgcgacagt gtcgtcgagc tggctgctaa
     4021 gtttcccgac gaagtccgcc gcggcctgag gttgaaaaag cacggcaacg agctcttgga
     4081 ggtgttagga ggacgagcga tccatccgat caacgtcgcc gttggcggat tctatcgaac
     4141 gccccgccgt gaggagctgg atcggttggt gccgagtttt gagtggggat tggaagctgc
     4201 gattgccgcc acccgatggg tcgcgacgtt cgactttccc gcagcgcagc gcgactacga
     4261 gatggtggcg ctgcatcatc ccgatgaata cgccttgaat gaagggcgca tcgcctgcac
     4321 ttccggactc gatatcccag tggaggaata tgaggctcat tttgaggagc ggcaggttcc
     4381 ccattcgacg gcgctgcact cggttcgact caattccgat cgcccctacc tcgtggggcc
     4441 gctggcgcgc ctggcgatca gccgccagca actgggggca tacgccaggc agcttgcgga
     4501 cgagcttggc tttcccacac cgtgtcgaaa tccatacttg agcatcgtcg cccgcggcct
     4561 cgaagttgtt catgcctacg aggaagcgct gcaaatcttg cgcacctatg tgccacccgc
     4621 catcagccgc attccgtatt cgcctcgtgc ggccagcggt tgcgctgcga ccgaggcacc
     4681 gcggggactc ttgtatcatc ggtacgagat caatcgcgaa ggcaaactcg tgctggcaaa
     4741 aatcgtgccg ccaacgtcgc agaatcaatg gcaaatcgaa gacgatctat tggccctgct
     4801 gccagacttc ttgagccgcg gcgatcagga ggtggccatc aactgcgagc gagcgattcg
     4861 caattatgac ccgtgcatta gttgttcaac gcattttctc aagctgaaga tcgacggatg
     4921 ttagcatgca aacgacctgc atcgtcggcc tgggctcgac acatggagat gaccagtttg
     4981 gctggttggc ggcggaccgc ctggcgcttg aaatcgagtt gcaccacatc gctgacgtga
     5041 ccgtgcgccg cgcgacagcc ccggcccaat tgctcgattg gcttacgggt attgaccagt
     5101 tgctgctcat cgatgcctgt cgccagccgg gcaggtgcgg tgaattgcag cagctcaact
     5161 ggcctgcacc agaagtagag gaactcactg gcagcggcag ccacagtttc tcggtgtggc
     5221 agacgcttac cttggcttcc gagctgggta cgctgccgcc gacgtgccgc atttggtgcg
     5281 ccgcgggaga gcggtttgaa gcagatcagg acctttcacc ttctctgctg gcactgctac
     5341 cgcgggtggt cgatgatgtg cttaggtggc tggcacaatg agattgtcgt atgcacgaat
     5401 cgtcactggt ccgcagtctg ctggagcgag tcagggaggt ctgcgagccg gccccactgg
     5461 ccgaggtcga ggaagtggtt gtggccatcg gcccgctcgc aggcgtcgag ccgctgctcg
     5521 tcgcgagcgc gttcgagctg cagacgcgcg ataccggact ggaacagacc cgcctgcgca
     5581 tcgagcaagt tccgctgcgc ctgacatgcg tctcttgcgg cgccgcatgg gagcgagacg
     5641 aaattgagtt tgcttgcccg gcgtgtaact gcaatcagac gcgcgtcgtg tcgggcgaag
     5701 gcatgatcat aaggcatatt gtgctcggcg atccagagag ggtgatgaaa tgagccgcgt
     5761 catagttgtc gagcgcgacg tgcagagttc tgcccgtgct tcggcggaag agtttcgcag
     5821 gcagcggacc agcgccggca cgctcgtggt caacctggtg tcttcgcccg gcgcaggcaa
     5881 gacgtcgctg cttcaggcca cggcccgccg atggacaggt ctatatcgcc tggccgtgct
     5941 cgttggcgat ctggccaccg atcgcgacgc gcagcggctc gcgccgctgg tcgaagtcgt
     6001 gcagatgacc actggcggcg cctgtcattt ggagttgccg ctggtcgagc gggcgtttgg
     6061 ccgattggcg tcaccagagc tggattttct gttcatcgaa aacgtgggca atctcgtttg
     6121 tcccgccagc cacgacctgg gcgagcacct gcgggtttgt ttgctcagca cacccgaagg
     6181 ggacgacaag cccggcaagt atcccaagat gttccgcggc agcggtgccc taataattag
     6241 taaaactgac ctcttgccac acgtgccgtt ttcgctcgat gcggccgcgg acgacgcgcg
     6301 ccagatccgc cctggtctgc ctgtctttcc cgtgtcgtcg ctgaatggca gcggcctggc
     6361 cgcctggtgt catttcctgg aggagcagcg caaggtgcgc gtcccaaaag tccgatgagt
     6421 acttccgctg ttgtcaaacg cgaggccggc gtcgtcgatc ggattcttgc cgtgcagttg
     6481 agcgttgccg ggtgcgtgca gggcgtcgga ctgcggccca ccatcgcccg acttgcggcg
     6541 cagctcgacc tggcgggcca tgtgcgcaac actccggccg gcgtagtgct cgttctggaa
     6601 ggctcgccag ccgccattga cgcctttgtg cccgcgctgg gcgcgcaatt accgccggag
     6661 gcaagagttc gcagcattga gcgcacggaa atcgcgtcca ggggtcgatc cggctttcag
     6721 atttgtgccg catcggtcga aggcgccgtc tcggcgccga ttcctctcga tatggcaacg
     6781 tgcccgcgct gcttggcgga tatcgccgag cctggcaatc gtcgttacgg ttatgccttc
     6841 acgagctgca ccgactgcgg acctcgatac tcgatcattc gcgagcttcc gtttgag
//
LOCUS       DASUGB010000263         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_724890, whole genome shotgun sequence.
ACCESSION   DASUGB010000263 DASUGB010000000
VERSION     DASUGB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724890"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="VFV87_05090"
     CDS             <1..1697
                     /locus_tag="VFV87_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5103161.1"
                     /translation="KYTSVESSVATVDERGKVTIAGPGEAAIKVWYLNYSAMAFLSVP
                     YANEVAPEVFAGAPRRNFIDDHSLAKLQALRVPPSPRCDDATFLRRAYLDTIGTLPTA
                     EEARAFLADTSADKRDRLIDGLLARPEFVDYWTYKWSDVLLLSGERLRPIALQTFYAW
                     IHESVAANKPWDQFVREIVTATGNTHEYGPANFYALHQDPEEMAETTAQAFMGLSINC
                     AKCHNHPLEKWTNDQYYGFANLFSRVRAKGWGGDYRGGDGLRIVYSDTQGELIQPSRG
                     LPQPPTPLDGEPLAFDDASDRRLAMADWLTSPENPYFTRAVANRVWANFFGIGLVEAV
                     DDMRLTNPASNEELLSAASQFLVENRYDLKQLMRAILQSETYQRASEPLSENKADERF
                     YSRYCVKRLKAEVLLDAISQATGVPTDFVAATPDSRKPGEPIKDIKRAIQLRDGYVDS
                     YFLAAFGKPDRLITCECERSNEPSMTQVLHLYNGDTVNKKLASAGSAAERAAAETDNA
                     KIVENLYLAALSRLPTEEEQQPLILELANTKPEERRQAIEDLYWSVLTSREFLFNH"
     gene            1714..2403
                     /locus_tag="VFV87_05095"
     CDS             1714..2403
                     /locus_tag="VFV87_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5103162.1"
                     /translation="MIAIISDIHGNLEALEAVLADIAQCGAEQTYCLGDIVGYGPNPC
                     ECLDIVATRCSKSILGDHEAALWSEYPAARSEALAEVAERTRGQLEHGPGGADARWEF
                     LWSLRPSFKERGMLFVHGSPREPTHEYVFPEDCYNTRKLDAIFDNIEWLCFSGHTHLP
                     GVFPYLDGFLRPEELAFEYELGELKSIVNVGSVGQPRDGDLRACYVLLDDRRVWFRRV
                     EYDWKKTQRKL"
     gene            2431..2676
                     /locus_tag="VFV87_05100"
     CDS             2431..2676
                     /locus_tag="VFV87_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103163.1"
                     /translation="MQREDVLKLWRQRPFRPFRIITVVGEKIDVMHPGLMLVAGDTIT
                     IGTPDPNFPPPAADELLWLDFEHIAEAEPLDSVSPRS"
     gene            2712..2969
                     /locus_tag="VFV87_05105"
     CDS             2712..2969
                     /locus_tag="VFV87_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103164.1"
                     /translation="MRRIDLVKLLHRRPFKPFRLTTTSKDSYDVKHPEAAIVGRRTLA
                     VWLPPLPTLGTSPPEFDPAEADVAWIDVLHIAQIEPIRGEK"
     gene            2966..5830
                     /locus_tag="VFV87_05110"
     CDS             2966..5830
                     /locus_tag="VFV87_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5103165.1"
                     /translation="MMNVISCSSRSAGAISCVLVFASCLAGADEAPDFTTDIAPLFTK
                     YCTGCHNDDDREGKLSLASYDSLLKGGEKGGVVTPQQPELSRLVRVLTGQAEPKMPPD
                     GEAAPKAEEIEVLKAWIAAGAKGPSGAGPDRAMLVTPEIKLLAPAKEIIHAVALAPDG
                     DLLAIARHGEVELQSLAEGKTVRKLTGIRGSVNGVAFSRDGQRISAAGGEPGLIGEAC
                     VFKTADGSLVNEFRGHKDSLYAAVLSPDATILATAGYDSAIKLWNVADGKEVRTLDGH
                     NGPVFELAFRADGNVLASASGDRTVKLWNVATGERLDTLKESTKELYTLAFSPDGSRL
                     AAAGVDNRIRVWRISPAAKEGTNPLLETKFAHDTPVTRVVWSADGRTIASCGEDRLVK
                     LWNADTLTIRETLEKQSDWASGLALGAKSDKLIVGRVDGSLGVYPLAAAGAETEQPLV
                     PLAEVPPEVDYGPQPELDKLPKVAEAEPNDEPAHATAIQVPGIATGRVFGGDRNQPLD
                     DDLFRFDAQAGDQWIIETKAARMKSPLDTKIEVLTADGQPVPRLLLRAVRDSVIEFRG
                     MNSDQRGVRLAHWEEMLLNEYVYLSGEVIKHFQQRRGPDGDANFYPENGNRLAYFDTT
                     SRAHSLGEPGYIVVPYAVGTDLPNNGLPVFTVNYENDDDAQRKLGKDSRLTFVAPADG
                     TYLVRVSDVRGFAGDNYNYELTVRRPQPDFTVKLTGENPTIGAGSGKAFTVKAERIDS
                     FNGPIRVDIAGVPPGFAVTTPLTIAEGLYEAYGVINALADAAAPTEEQLKAIKITATA
                     TVCGQERTKDVNSLGTIKLADKPKVIAYLEPLAGGKLAPGEAAQDSPSETAGPGLVPA
                     ISIAPGGTVTLNLRVERSGFDGRIPFEVANLPHGIIVDDIGLSGVLVREKELERPIVL
                     RAEPWVAEQDRTFQATAQVEGNQSTLPLLLTVRHEDTGPP"
     gene            complement(5836..>6668)
                     /locus_tag="VFV87_05115"
     CDS             complement(5836..>6668)
                     /locus_tag="VFV87_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103166.1"
                     /translation="KVISGGLPEGDAAFKELAELGVKTVISVDGAKPDVQAAKKYGLK
                     YVHMPHGYDGVPELRAKELAKAVRDLPGPIYIHCHHGEHRSPAAATVACVGAGLLAPD
                     AALGVLKAAGTSESYRGLYQSAVSAKKLDDALLDELKVEYPETVRVPPIADAMIELEH
                     AHDHLKALAANNWKILQDQPALEPAHEALLLREHCTELLRTDEVRQQPEGFRMLLSES
                     QVIARQLEDLLTEWQKAGSPSPPPEKLGKAFDAVTQSCATCHKTYRDVPLAEKRSSPR
                     "
BASE COUNT         1293 a         2101 c         2093 g         1181 t
ORIGIN      
        1 ccaagtacac cagcgtggaa agctcggtcg ccacggtcga tgagcgcggc aaggtgacga
       61 ttgccggccc gggcgaagcg gcgatcaagg tctggtatct caactatagc gcgatggcgt
      121 ttctcagcgt gccgtatgcg aatgaagtcg cgccggaagt ctttgcgggc gccccgcggc
      181 ggaatttcat cgatgaccac tcgctggcca agctccaggc gctgcgcgtg cctccctcgc
      241 cgcgctgcga tgacgcgacg ttcctccgcc gcgcctatct cgacaccatc ggcaccctcc
      301 cgaccgccga ggaagcccgc gcctttctcg ccgatacatc agccgacaag cgtgaccggc
      361 tgatcgacgg gctgctcgcg cggccggagt tcgtcgatta ttggacgtac aagtggtccg
      421 acgtgctgtt gctttccggc gagcggctgc ggccgatagc tttgcagacg ttctatgcct
      481 ggattcacga aagcgtcgcc gcgaacaagc cgtgggacca gttcgtccgc gagatcgtga
      541 cggcgaccgg caacacgcac gaatacggcc cagcaaattt ttacgccctg caccaggacc
      601 cggaggagat ggccgagacg acggcccagg cgtttatggg cctgtcgatc aactgtgcca
      661 agtgccacaa ccatccgctg gagaaatgga ccaacgacca atactacggc ttcgcgaatc
      721 tgttctcgcg cgtgcgggcc aagggttggg ggggcgacta tcgcggcggc gatgggctgc
      781 ggatcgtcta ttccgacaca caaggcgaac taatccagcc cagccgcggc ctcccgcagc
      841 cgccaacgcc gctcgatggc gagccgctgg cattcgatga cgcgagcgac cgccgcctgg
      901 cgatggccga ttggctcacg tcgccggaaa acccgtactt cacgcgggcc gtggccaatc
      961 gcgtctgggc caacttcttt ggcattggtc tggtcgaagc ggtcgatgac atgcgactga
     1021 cgaacccagc gagcaacgag gagctgctct cagccgcgtc acagtttctc gtcgagaacc
     1081 gctacgacct gaagcagctc atgcgggcga tcctgcagtc ggaaacctac cagcgggcca
     1141 gtgagccgct gtcggaaaac aaagccgacg agcggttcta ctcgcggtat tgcgtcaagc
     1201 ggctgaaggc ggaggtgctg ctggatgcca tttcgcaggc gaccggtgtg ccgaccgact
     1261 ttgtcgccgc cacgcccgac agccgcaagc ccggcgagcc gatcaaggac atcaagcggg
     1321 cgatccagct ccgcgacggc tacgtcgatt cgtactttct ggctgccttc ggcaagcccg
     1381 accggctgat cacctgcgaa tgcgaacgga gcaacgagcc gagcatgacg caggtgctgc
     1441 acctctataa cggcgacacg gtcaacaaga agctcgccag cgcgggtagc gccgccgagc
     1501 gcgccgccgc cgagaccgac aacgccaaga tcgtcgaaaa cctctatctc gccgcccttt
     1561 cgcgcctgcc caccgaagaa gaacagcagc cgctgatcct ggagctcgcc aacaccaaac
     1621 ccgaagagcg ccggcaggcg atcgaggacc tgtactggag cgtgctgacg agtagggagt
     1681 ttttgtttaa tcactgagct gacgcattcg gtcatgatcg ccatcatcag cgacattcac
     1741 gggaacctgg aagcattgga agcagtgctg gccgacattg cgcaatgtgg tgcagagcag
     1801 acttactgcc tgggagacat cgttggctac gggccaaatc cgtgtgaatg tctcgacatc
     1861 gtggcgacac ggtgttccaa atcgattctc ggtgatcatg aagctgccct gtggagcgag
     1921 tatccagcag cacggagtga ggcgctcgct gaagtggccg agcgaacacg cggccaactt
     1981 gagcacggtc caggaggcgc tgacgcacgc tgggagttcc tttggagcct gaggccttcg
     2041 ttcaaagaga ggggcatgct gtttgtgcat ggttcgccgc gcgaaccgac acacgagtac
     2101 gttttcccag aggactgtta caacacccga aaactggatg ctatcttcga taacatcgaa
     2161 tggctctgct ttagtggcca tacccatctc cccggcgtgt ttccatattt ggatggtttt
     2221 cttcgtcccg aagaacttgc gttcgaatat gaacttggcg agctcaagtc gatcgtgaac
     2281 gtcgggagcg tcggacaacc tcgcgatggc gatctgcgcg cctgctacgt gttgctcgat
     2341 gaccgccgtg tctggtttcg gcgagttgag tacgattgga agaagacgca gcgcaagttg
     2401 tagcgacgtt cgactgagga gtttggtgcc atgcaacgcg aagacgtatt aaagctctgg
     2461 cgtcagcggc cgtttcggcc gttccggatc atcaccgttg tcggcgagaa gatcgacgtg
     2521 atgcacccgg gtctaatgct cgtcgctggc gacaccatta cgatcgggac gcccgacccc
     2581 aactttccgc cgccggctgc cgacgagctg ctgtggctgg atttcgagca tattgcggag
     2641 gcagaaccgc tggattctgt ttcgccgcgg agttagccca acatctgttg aatcatttgg
     2701 aggcgggcca catgcggcgg atcgatctgg tgaagttgtt gcatcggcgt ccattcaagc
     2761 cgttccgcct gaccacaact tccaaggaca gttatgacgt gaagcatccc gaggccgcga
     2821 ttgtgggccg gcgcacgctt gcagtctggc tgccgccgct ccctacgttg ggcacatcgc
     2881 ccccggaatt tgatcccgca gaagcagacg tggcttggat cgacgtgttg cacatcgctc
     2941 aaattgagcc gattcgaggc gagaaatgat gaacgtcata tcgtgcagtt cgagaagtgc
     3001 tggcgccatt tcctgcgtcc tcgttttcgc atcctgtttg gccggcgcgg acgaagctcc
     3061 cgacttcacc actgacatcg cgcctctctt caccaagtac tgcaccggct gtcataacga
     3121 tgacgaccgt gaggggaagc tgtcgctggc aagctacgac tcgctgctga agggcgggga
     3181 gaaggggggc gtcgtcacgc cccagcagcc ggagttgagc cgcttggtgc gcgtgctgac
     3241 cgggcaggcg gagccgaaaa tgccgccgga cggcgaagcc gcgccgaagg cggaagagat
     3301 tgaggtgctg aaggcgtgga tcgcggcagg ggcaaaaggg cccagcggcg ctggcccgga
     3361 cagggcgatg ctcgtgacgc ccgaaatcaa gctgctcgcg ccggccaagg agatcatcca
     3421 cgcggtcgct ctcgcaccgg atggcgattt gctggcgatc gctcggcacg gcgaggtcga
     3481 gctgcagtcg ctggccgagg gaaagacggt tcgcaagctg accggcattc gcggcagcgt
     3541 gaatggagtt gccttttcgc gagacgggca acgaatatct gcagcgggcg gcgagccggg
     3601 gctgatcggc gaggcttgcg ttttcaaaac cgccgacggc tctctcgtca acgagttccg
     3661 cgggcacaaa gacagcctct atgccgccgt gctcagcccc gatgccacaa tcctcgccac
     3721 cgccggctat gacagcgcga tcaagctgtg gaacgtggcc gatgggaagg aggtccgcac
     3781 gctcgatggg cacaatggcc cggtgttcga actcgctttc cgggccgacg gcaacgtgct
     3841 ggccagcgcc agcggcgacc gcacggtgaa gctctggaat gtcgccaccg gcgagcggct
     3901 cgatacgctc aaggaatcga ccaaggaact ctatacgctg gccttcagtc cggatggctc
     3961 gcggctggcc gcggctggcg tcgataaccg gattcgcgtc tggcgaatct cgcccgcggc
     4021 gaaggaaggg accaatccgc tgctggagac gaaatttgct cacgatacgc ccgtcacgcg
     4081 cgtcgtctgg tcagccgatg gccggacgat tgcctcctgc ggcgaagacc ggctggtgaa
     4141 gctgtggaat gccgacacgc tcacgattcg cgaaacgctc gaaaagcaaa gcgactgggc
     4201 cagcggcctc gctctgggcg cgaagagcga caagttgatc gtcggccgcg tcgatggcag
     4261 cctgggcgtc tatccgctgg ccgccgccgg cgcagaaacc gagcagccgc tggtgccgct
     4321 ggccgaagtt ccgcccgaag tcgattacgg accgcagccg gagctcgaca agctgccgaa
     4381 ggtcgccgaa gcggagccga acgacgagcc ggcccacgca acagccatcc aggttcccgg
     4441 catcgcgacc ggccgcgttt tcggtggcga ccgcaatcag ccgctcgatg acgatctgtt
     4501 tcgcttcgac gcccaagcgg gcgaccagtg gatcatcgag accaaggcag cccggatgaa
     4561 atcgccgctc gatacgaaga tcgaggtcct caccgccgat gggcaaccgg tcccgcggct
     4621 gcttttgcgg gcggtccgcg actcggtcat cgagttccgc ggcatgaaca gcgaccagcg
     4681 gggcgtgcgg ctggcccact gggaggagat gctgctcaac gagtacgtct atcttagcgg
     4741 cgaggtcatc aagcacttcc agcagcgccg cggccccgat ggcgacgcca atttctatcc
     4801 cgaaaacggc aatcgcctcg cctatttcga caccaccagc cgggcccatt ccctgggcga
     4861 gccgggctac atcgtcgtgc cgtatgcggt cggcacagac ctgcccaaca atggcctgcc
     4921 agtcttcacg gtcaattacg agaacgatga cgacgcccag cggaagctcg gcaaggattc
     4981 gcggctgaca tttgtcgcgc cggcggacgg aacgtacctc gtccgcgtca gcgacgttcg
     5041 cggcttcgca ggggacaact acaactacga gctgaccgtc cgccgcccgc agcccgattt
     5101 caccgtgaag ctcaccggcg aaaacccgac gattggcgcc ggcagcggca aggcgttcac
     5161 ggtcaaagcc gagcggatcg acagtttcaa cggcccgatc cgcgtcgata tcgcgggggt
     5221 gccgccggga ttcgccgtga cgacgccgct gacgatcgcc gaaggactct acgaggctta
     5281 cggcgtcatc aacgccctcg ccgacgccgc cgctccaacg gaagagcagc tcaaagcgat
     5341 caaaatcacg gccaccgcca ccgtctgcgg ccaggagcgg acgaaagatg tgaacagcct
     5401 cggcacgatc aagctcgccg acaagccgaa ggtgattgcc tatctggagc ccttagccgg
     5461 gggcaaactt gctcccggag aagcggcgca agattctcca tccgaaacgg cgggaccagg
     5521 tttggtcccg gctatttcga tcgcccccgg cggcaccgtc acgctcaacc tccgcgtgga
     5581 gcggagcggc ttcgacggcc gcattccgtt tgaggttgcc aacctgccgc acggcatcat
     5641 cgtcgatgac atcggcctct cgggcgtcct ggtgcgcgag aaggaattgg agcgcccgat
     5701 cgtcctccgc gccgagccct gggtcgccga gcaggaccgc acgttccaag ccaccgccca
     5761 ggtcgaaggc aaccaatcca cgctgccact gctactcaca gtgcggcacg aggacacggg
     5821 accaccgtga tgcaatcacc gtggcgatga gcgcttttcc gccagcggca cgtcgcggta
     5881 ggtcttgtgg cacgtggcac acgactgcgt gacggcgtcg aaggctttgc ccagcttctc
     5941 tggtggaggc gaaggcgagc cggccttttg ccactcggtc agcaagtcct caagttgccg
     6001 ggcaatgacc tgactttcgc tcagaagcat gcggaaaccc tcaggctgct gccggacttc
     6061 atccgtgcgc aagagctcgg tgcagtgctc gcgcaagagg agcgcttcgt gcgccggctc
     6121 cagagcaggc tggtcctgga gtattttcca attgttcgcc gccagggcct tcaggtggtc
     6181 atgagcatgc tccaattcga tcatggcgtc ggcgatcggc gggactctga cggtctcggg
     6241 gtactcgact ttcagctcat cgagcagcgc atcatccagc ttcttagccg atacggcgga
     6301 ttggtaaagt ccgcgatagc tctcgctggt gccagccgct ttcaatacac caagtgctgc
     6361 atccggcgcg aggaggcccg cgccgacgca cgccaccgtc gccgcggccg ggctgcggtg
     6421 ctcgccgtgg tggcagtgaa tgtaaattgg ccccggcaga tcgcgaaccg ccttggccaa
     6481 ttccttggcc cgcaattcgg gcacgccgtc atagccgtgc ggcatgtgga cgtacttcag
     6541 tccgtacttt ttggctgcct gaacgtcggg cttcgcccca tcgacgctaa tgactgtctt
     6601 cacgcccagt tcggccaatt ccttaaacgc agcatcccct tcgggcagtc caccggaaat
     6661 caccttcg
//
LOCUS       DASUGB010000264         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1003563, whole genome shotgun sequence.
ACCESSION   DASUGB010000264 DASUGB010000000
VERSION     DASUGB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003563"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFV87_05120"
     CDS             complement(<1..625)
                     /locus_tag="VFV87_05120"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5103167.1"
                     /translation="MSETASKIVEQLKSLGSESTKKVLMRHGAKEPFFGVKIEHLKKI
                     QKRIKRDYQLALDLYDTGISDAMYLAGLIADDAKMTKRDLQKWVKGAYWYMLSEYTVP
                     WVAAGSKHGHDLALEWIESKNELVASAGWATLSSLVSTKDDAELDIPELKKLLARVEK
                     TIHKQPNRVRGAMNAFVISVGSYVKPLLKQAKAAAKAIGVVSVDMGET"
     gene            complement(664..>2061)
                     /locus_tag="VFV87_05125"
     CDS             complement(664..>2061)
                     /locus_tag="VFV87_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5103168.1"
                     /translation="EDGLAQSKIRQGVTTEVLGEGTSAGPRVGKLSPQQMLVGGKPVE
                     WRTLGEYFGAVEEAKISVNIASYVGLDNVWKAVLGESQERPTAEQLAEMQQLVAAAMD
                     DGAMGLSTMLAMPPGSIATTDDIVALCEPVAKRGGIYSTHNRNEGTGVFDAIKEAIEI
                     GERAGVPVDIIHVKIADQQYWGRMGEIIELIEAARARGVNVQTNIYPYTRGNNNLVSI
                     VPPWAHDGGKGKLLERLKDAGERERMKRDIRGGIPGWYNHFTAVGGDWSRMLISGKCQ
                     YEGLTMDRVISLRTKGIDPPPDSLDVLFDLLIEEEGSVSTVYAHHTEADMNLALQQPW
                     CSIGSDGSALSLDGELRRGNPHPRNFGTFPRVLGVYVREQKLLTLEDAVRKMTSLNAA
                     KLGVRDRGLIREGMQADITVFDPATVIDKATYTAPFAYNDGIEYVIVNGQVVLDRGRH
                     TGAKPGRVLRKRDGK"
BASE COUNT          317 a          677 c          623 g          444 t
ORIGIN      
        1 cggtttcgcc catatcgacc gatacgaccc cgatcgcttt ggctgcggct ttcgcttgct
       61 tcaacaatgg tttgacatag ctgccgacgg aaatgacaaa cgcattcatc gcgccgcgga
      121 cgcgattggg ctgcttatgg atcgtcttct ccacccgcgc cagcagcttt ttcagctcgg
      181 gaatgtccag ctcggcatcg tccttggtcg agacgaggct gctgagcgtc gcccagccag
      241 ccgaggcaac cagctcgttt ttcgactcga tccactccag agccaggtcg tgcccgtgct
      301 tgctccccgc ggcgacccac ggcacggtgt attcgctgag catgtaccag taggcccctt
      361 tgacccactt ctgcaggtcc cgcttcgtca tcttcgcatc gtcggcgatc agcccggcca
      421 ggtacatcgc gtcggaaatc cccgtgtcgt acaggtcgag cgcgagctga tagtccctct
      481 tgatccgctt ctggatcttc ttcaggtgct cgatcttgac cccgaagaac ggctccttgg
      541 ccccgtgccg catcagcacc ttcttcgtgc tctcgctgcc gagggatttc agctgttcca
      601 cgatcttcga agcggtttca ctcatggctg caaatccgtg ttggatccgt gttccgtccg
      661 tggctacttg ccgtctcgtt ttcgcagcac ccggccgggc tttgcgccgg tatgccggcc
      721 gcggtcgagg acgacctgcc cgttgacgat gacgtactcg atgccgtcgt tgtaggcgaa
      781 gggcgcggta taggtggctt tgtcgatgac cgtcgccgga tcgaagacgg taatgtcggc
      841 ctgcatgcct tcgcggatca gtccccgatc tcgaacgccg agcttcgccg cattcagcga
      901 cgtcatcttc cgcaccgcgt cttccagcgt cagcagcttc tgctcgcgga catagacacc
      961 caggactcgc ggaaacgtgc cgaagtttcg cgggtgggga ttgccgcggc ggagctcgcc
     1021 gtcaagcgac agggccgagc cgtcggagcc gattgagcac cagggctgct ggagggccag
     1081 gttcatgtcg gcttcggtgt gatgggcata gacggtcgag acggagccct cttcttcgat
     1141 caagaggtcg aagagcacgt cgaggctgtc gggcggcgga tcgatcccct tggtccgcag
     1201 cgaaatcacc cggtccatcg tcagcccttc gtactggcac tttccactga tgagcatccg
     1261 cgaccagtcg ccgccgaccg ccgtaaaatg gttgtaccag ccgggaatgc cgccgcgaat
     1321 gtcgcgcttc atccgctccc gctcgccggc gtccttcagc cgctccagga gcttgccctt
     1381 gccaccgtcg tgggcccagg gggggacgat gctgacgagg ttgttgttgc cgcgggtgta
     1441 gggatagatg ttggtctgga cgttgactcc gcgggcgcgg gccgcctcga tcaactcgat
     1501 gatctcgccc atccggcccc aatactgctg gtcggcgatt ttgacgtgga tgatatcgac
     1561 cggcacgccg gcccgctcgc caatctcgat cgcttccttg atcgcgtcaa acacgccggt
     1621 tccttcgttg cggttgtgcg tggagtaaat cccgccgcgc ttcgcgaccg gttcgcacag
     1681 agcgacgatg tcgtcggtcg tggcgatcga gccggggggc atggccagca tcgtcgaaag
     1741 gcccatcgcg ccatcgtcca tcgccgctgc gacgagctgc tgcatttccg cgagctgctc
     1801 ggctgtcggc cgctcttgcg attcgccgag gaccgccttc cagacgttat cgaggccgac
     1861 atacgatgcg atgttgaccg agattttcgc ttcttcgacg gcgccgaagt attcgcccag
     1921 cgtccgccac tcaaccggct tgccgccgac gagcatctgc tgcggcgaga gtttgcccac
     1981 gcgcggcccg gctgaggtgc cttcgccgag gacttcggtc gtcaccccct ggcgaatttt
     2041 gctctgggcc agcccgtcct c
//
LOCUS       DASUGB010000265         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_725043, whole genome shotgun sequence.
ACCESSION   DASUGB010000265 DASUGB010000000
VERSION     DASUGB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725043"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1386)
                     /locus_tag="VFV87_05130"
     CDS             complement(13..1386)
                     /locus_tag="VFV87_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5103169.1"
                     /translation="MALVLTGQQLGYIEPCGCTGLENQKGGLARRHALLRQLTEERGW
                     TVVPLDVGSQVRRFGKQAEIKFRHSADALRLMGYKAVALGADDLRLPADALLSGTNPA
                     EETSVFVGSNVALISRDLQPTFTIIEAGGKRIGVTAVLGDKYEQRLQSDLLVHAPAVE
                     SLKSACTELKQSNCDLYVLLAHAPPEEARKIAEEVPIFDLVVVAGQTAVPSQELEAVP
                     GTKTKLVQTGTKAMYAGVVGVFDDRDRRLRFESVPIDAKLADSPPMLKMLSDYQQQLQ
                     DILQDEGLEGLGLTPRPIASGRKFVGSEKCGQCHTKAFAIWRETPHAHATDSLVHPPN
                     SRGSIARHFDPECLSCHVTGWEPQQFSPLESGYLSLEKTPDLQHNGCENCHGPGSAHV
                     AAETGDAPANEAEMTKLRDEMKLPLAGGVAEKKCLECHDDDNSPDFHHQGAFDEYWKQ
                     VEHRGKD"
     gene            complement(1719..>2545)
                     /locus_tag="VFV87_05135"
     CDS             complement(1719..>2545)
                     /locus_tag="VFV87_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103170.1"
                     /translation="TEIKMEWNAKKGVDPFEQYAAFRTNDPRRPEFKLIVFGQILETL
                     RSEPPEAIFNRVSSQESARATLKVFGLRGDKLEIIRHQFEDEPSAKFFEVSFQPLPQE
                     EVSKRKGFKCGIEIIVELKPGMPLGQMKQTIQITTNLNPDATFDIPVYGSVVSDISLV
                     GQHTSAESMTVSMGTIASAKGAKATVYFVVKGEHRDQTTLQITKVSPSAELEVKLGEP
                     IRDNPQIVSFPVTLEVPPGTAPVSRASAGGEVTVRVETTHPSVKELTFRVRYAVVE"
BASE COUNT          432 a          782 c          799 g          532 t
ORIGIN      
        1 ggtccttccg ccctaatctt tgccgcggtg ctcaacctgc ttccaatact cgtcaaacgc
       61 gccctggtgg tggaagtccg ggctgttgtc gtcgtcgtga cactccaggc acttcttctc
      121 cgcgacgccg ccggccagcg ggagcttcat ttcgtcgcgc agcttcgtca tctcggcctc
      181 attcgccggc gcgtcacccg tttcggccgc gacgtgggcg gagcctggac catggcaatt
      241 ctcacagcca ttgtgctgca gatcgggcgt tttctccagg ctcagatagc ccgactccag
      301 cgggctgaac tgctgcggct cccagccggt gacgtggcag ctcaaacact ccggatcgaa
      361 gtgccgggca atgctgccgc gggaattggg cggatggacg agcgaatcgg tcgcgtgggc
      421 atggggcgtc tctctccaga tcgcgaaggc cttggtgtgg cactggccgc atttttcact
      481 gcccacaaac tttcgtccgc tcgcgatcgg acgcggcgta aggcccaggc cttccagacc
      541 ttcgtcctgc aagatgtcct gcaattgctg ctgataatcg gacagcattt tcagcatcgg
      601 gggcgaatcg gcgagcttcg catcgatcgg gacgctttcg aagcgcagcc gccgatcgcg
      661 gtcgtcgaaa acgccgacca cgccggcgta catcgccttg gtcccggtct gcaccaactt
      721 cgttttcgtg cccggcacgg cttccagttc ctgcgacgga accgccgttt ggccggcgac
      781 gacgaccagg tcgaaaatcg gcacttcctc cgcaatcttg cgggcctctt cgggcggagc
      841 gtgcgccagg agcacgtaca ggtcacagtt gctctgtttc aattccgtgc aggcactttt
      901 aagcgactcg accgcgggcg catgcaccaa aaggtccgat tgcagccgct gctcgtactt
      961 gtcgcccaaa acggccgtga cgccgatccg cttgccgccg gcttcgatga tggtgaacgt
     1021 cggctggagg tcgcgggaga tgagagcgac gttggaaccg acgaacacgc tggtctcttc
     1081 cgcgggattg gtgccgctga ggagcgcgtc agcgggcagg cggaggtcgt cggcgcccag
     1141 ggcgaccgct ttgtatccca tcagccgcag ggcgtccgcc gagtgccgga acttgatttc
     1201 cgcctgcttg ccgaagcgcc gcacctggct gccgacgtcg agcggcacaa cggtccagcc
     1261 ccgctcctcg gtaagttgcc tgaggagcgc gtggcggcgg gccaggccgc ctttctggtt
     1321 ttccaggccc gtgcagccgc acggctcgat gtagccgagt tgctggccgg tcagcacgag
     1381 ggccacggcg ggcttggacc agccgtcgaa gagagggggc gggccgggag gacttgaagg
     1441 agaatctggc ggtccgtgcg gagggtcgcc cccggattgc aaattcttgg cggtcgcttg
     1501 gccagcgtta tcgacagcgg gttgccccga tttggctggg agcgggactg catcattgca
     1561 accgagtgcc gccgcgagca aaccagcgat caagggtggc gctacgagag ttcgaagctg
     1621 gttcaccgat gcagaatgcg tcatgatgat tcgtccttga atgctggcgg cacaacgtcg
     1681 ccggccggtc gctgttcggc gcgtgcagtg gttactggtt attctacgac tgcgtatcgc
     1741 acgcggaacg tcaattcttt gacgctggga tgggtcgttt caacgcgcac tgtcacctcg
     1801 ccaccggccg aggcccggct gacgggggcg gtgcccggcg gcacttcgag cgtcacggga
     1861 aagctcacaa tctgcggatt gtcgcggatt ggctcgccaa gtttcacttc caattcggca
     1921 gagggcgaaa ccttggtgat ctgcagtgtc gtctgatcgc gatgttctcc cttgaccaca
     1981 aaatagactg tcgccttcgc tcccttggcg gatgcaatcg tccccatcga cacggtcatc
     2041 gattccgccg aggtatgctg cccaaccagc gaaatgtcgc tgacgacgct tccatagaca
     2101 gggatatcga acgtcgcgtc gggattgaga ttcgtcgtga tctgaatcgt ctgtttcatt
     2161 tgccctagcg gcatgccagg cttgagctcc acgattatct caatcccgca cttaaagccc
     2221 ttgcgcttgc tcacttcctc ttgcggcagc ggctggaagg aaacctcgaa gaactttgcc
     2281 gagggttcgt cctcgaactg gtgacggatg atctccaact tgtcgccacg gagcccgaag
     2341 accttcaaag tcgcccgcgc cgattcctgc gaagagacgc ggttgaagat cgcttcgggc
     2401 ggctcagagc gcaaggtctc gaggatttgg ccgaacacga tcagtttgaa ttcgggtcgt
     2461 cgcggatcgt tcgtgcggaa cgcggcgtat tgttcgaacg gatcgacgcc tttcttggcg
     2521 ttccactcca tcttgatttc ggtcg
//
LOCUS       DASUGB010000266         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1697645, whole genome shotgun sequence.
ACCESSION   DASUGB010000266 DASUGB010000000
VERSION     DASUGB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697645"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VFV87_05140"
     CDS             <1..1392
                     /locus_tag="VFV87_05140"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX5103171.1"
                     /translation="HLLDFWERLDGRQRGRLARQIEALDPDLFRKLRAEHAAGPAAGD
                     SPSKWAELAARAEPPPAMRLDGSGVEFTAQSARERGAAALAAGEVGMILVAGGLGTRL
                     GFDQPKGLFPLAPLSKRTLFQILIEQLLAVRRRCGVAIPLYVMTSEATDEVTRSFLGE
                     HANFGLPAADLHIFCQASMWAVDERFERILLEAPDSLFLGPDGHGGMLAALEKSGGLA
                     DAERRGIKHLFYGQIDNPLTQICDELFLGSHILAGSEMTTQVVRKREPLERVGNVVQV
                     AGRTQIIEYSDLPEEFARRTNPDGSLKLWAGNLAVHVFDVAFLQRQAADPQALPFHCA
                     KKKVTCLDSQGQLVQPERPNALRFERFIFDLLPRAKKALVVEVDPCEAFAPVKNSDDE
                     PTDNPRLAKLAMMSLDRERLRQAGALVADNVPVEINPLWAGTVEELRGRLARGTIIDQ
                     PTYFSPDGPRLIV"
     gene            1406..>2361
                     /locus_tag="VFV87_05145"
     CDS             1406..>2361
                     /locus_tag="VFV87_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX5103172.1"
                     /translation="MLHAVILAGGSGTRFWPLSRAARPKQLLDLTGGRTMIQATFDRL
                     AGLAPAERTLVVTNRSLVGAIAEQLPEVPRAKIVGEPCKRDTAPAIGLAALLVSKSDM
                     DATMISMPADHLIEPTEAFQAAIRQAAAIVQERPETFVTFGIKPTYPAESFGYIERGE
                     GSGFGVQGSGEREQGTGDRGQGTESRNNPQSAIRNLQFTVVRFREKPARSVAEEYLAS
                     GKFYWNSGIFVWKARTVLDAISRYEPQMGEQLQKIGAAVGRPDFEQVLDREFTAIRGK
                     SIDFAVMERYQPVVVIEAPFTWDDVGSWQALARAAGADADGNT"
BASE COUNT          395 a          757 c          835 g          374 t
ORIGIN      
        1 catctgctcg atttctggga gcggctcgat ggccggcagc gggggcgact ggcccggcag
       61 attgaagccc tcgatccgga cctgtttcgc aagctgcggg cggagcatgc ggctgggcct
      121 gccgccggcg attcgccatc gaaatgggcc gagctggccg caagggccga gccgccgccg
      181 gcgatgcgct tggacggcag cggggtcgag ttcacggcgc agtcggcccg cgagcgggga
      241 gcggcggcgc tggccgccgg cgaagtcggc atgatcctcg tcgccggggg cctgggaacg
      301 cggctcggct tcgatcagcc gaaggggctg tttccactcg cgccgctctc gaagcggacg
      361 ctgttccaga tcctgatcga gcagctcctg gcggtgcggc ggcgctgcgg cgtcgcgatt
      421 ccgctgtacg tgatgaccag cgaggcgacc gacgaggtca cgcggtcgtt cctcggcgag
      481 cacgcgaatt tcggtctgcc ggcggccgat ctgcacatct tctgccaggc gtcgatgtgg
      541 gccgtcgatg agcgattcga gcggattctg ctcgaagcgc ccgattcgct ctttttgggt
      601 ccggacggcc acggcgggat gctcgcggca ctggaaaaga gcggcggact ggccgatgca
      661 gagcggcgcg gcatcaagca cctcttttac ggccagatcg acaacccgct cacgcaaatc
      721 tgcgacgagc tcttcctcgg cagccacatc ctcgccggca gcgaaatgac gacgcaggtc
      781 gtccgcaagc gcgagccgct ggagcgcgtc ggcaacgtcg tccaggtcgc cggccgcacg
      841 cagatcatcg agtacagcga cctgccggag gaattcgccc gccggacgaa tccggacggc
      901 agcctgaagc tctgggccgg caatctcgcg gtccacgtct tcgacgtcgc cttcctccag
      961 cgtcaagccg ccgacccgca ggccctgccg tttcactgtg cgaagaaaaa ggtcacctgc
     1021 ctggactcgc aaggtcagct cgtccagccg gagcggccca acgccctccg tttcgagcgg
     1081 ttcatcttcg acctcctgcc gcgagcgaag aaggccctgg tcgtcgaagt cgatccgtgc
     1141 gaggcctttg cccccgtgaa gaattccgac gacgagccga ccgacaaccc gcggctggcg
     1201 aagctggcga tgatgtcgct cgaccgcgag cggttgcggc aggcgggggc gctggttgcc
     1261 gataacgtgc cggtggagat caatccgctc tgggccggca cggtggagga gcttcgcggc
     1321 cggctcgcgc ggggcacgat catcgaccag cccacctact tctcgccaga cggcccgcgt
     1381 ttgattgtct gaaaggacgt gcgacatgct tcacgccgtg attctcgccg gaggctcggg
     1441 aacgcgcttc tggcccctca gccgggccgc gcggcccaag cagctcctcg acctgaccgg
     1501 cgggcggacg atgatccagg cgacgttcga ccggctggcg gggctggcgc ctgcggagcg
     1561 gacgctggtc gtgaccaatc gcagcctcgt cggcgcgatt gccgagcaac tgccggaggt
     1621 tccgcgggcg aagatcgttg gcgagccgtg caagcgcgac accgcgccgg cgatcggcct
     1681 ggcggccctc ttggtatcga aatccgatat ggacgccacg atgatctcga tgccggccga
     1741 ccatctgatc gagccgaccg aggcgtttca ggcggcgatc cggcaggcgg cggcgatcgt
     1801 ccaggagcgg cccgagacgt tcgtcacgtt cggcattaag ccgacctatc cggcggagag
     1861 ctttgggtat atcgagcggg gggagggttc agggttcggg gttcagggtt caggggagag
     1921 ggaacagggg acaggggaca ggggacaggg gacagaaagc agaaacaatc cgcaatccgc
     1981 aatccgcaat ctgcaattca cggtcgtccg cttccgcgaa aagccggccc ggtcggtcgc
     2041 cgaagagtac ctggccagcg gcaagttcta ctggaacagc ggcatcttcg tctggaaggc
     2101 gcggaccgtg ctcgatgcga tcagccgcta tgagccgcag atgggcgagc agcttcagaa
     2161 gatcggcgcg gcggtgggcc ggcccgattt cgagcaggtc ctcgaccgcg agttcacggc
     2221 cattcgcggc aagtcgatcg actttgccgt aatggagcgc tatcagccgg tggtcgtgat
     2281 cgaggccccg ttcacctggg acgacgtagg gagctggcag gcgctggccc gcgcggcggg
     2341 agccgatgcc gacggcaaca c
//
LOCUS       DASUGB010000267         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_725344, whole genome shotgun sequence.
ACCESSION   DASUGB010000267 DASUGB010000000
VERSION     DASUGB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725344"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFV87_05150"
     CDS             complement(<1..224)
                     /locus_tag="VFV87_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103173.1"
                     /translation="MQDSRRPSLLRIWLAAACLLAAAAAFGAEPVKLLNLPGIAGEPG
                     RIEYGELPRVAGTLAVVSGAEVEEGAAKGQ"
     gene            1672..2748
                     /locus_tag="VFV87_05155"
     CDS             1672..2748
                     /locus_tag="VFV87_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103174.1"
                     /translation="MNKSTSADDVKLMSSYESLRGTLLSDKFRDRLDRQLAFWALPSD
                     RRLPTAFLHRTLDDLLSCSYKELASTAGIGHKKLQTFVKLLVRATKEDASNTTLDEIH
                     AAEEETPLAGLDSDGQFDPALVSEMVWIQWRETARRHGIGHELLGRLTGTLQSLPSVV
                     WNTPLSSYLDLSLAEMRDLKTHGEKRIGVVLEVFHNIHKMLGQADPAGGLAVRLAPRT
                     IASAEDWIAEVRSRSFPPGHEEIGQYLIEPILQQLAIDAGDTVAKLARGRLGVGAGTE
                     SVRHQSQSLGVTRARVYQMLEECHGVMQIRWPEGRRQLDDFGQWLDECYASAEAANLV
                     ASFRELLYPLKFDSVGEALRAEAV"
     gene            complement(2767..3225)
                     /gene="ruvX"
                     /locus_tag="VFV87_05160"
     CDS             complement(2767..3225)
                     /gene="ruvX"
                     /locus_tag="VFV87_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5103175.1"
                     /translation="MSDALPRQGRLAAIDYGTVRIGVAITDPEQRLASPLEIYTRRNP
                     AADAAWITQLVSSERIAGLVVGLPVHTTGGESQKSAEARRFAAWLSETTNLPVRLFDE
                     RFTTAHAESLLQDAGLTKKQRKERLDKLAAQILLAAYLESSRTPAEPGAL"
     gene            complement(3222..>3859)
                     /locus_tag="VFV87_05165"
     CDS             complement(3222..>3859)
                     /locus_tag="VFV87_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103176.1"
                     /translation="YASTRRSLDDHARSLARVIDNLNDAERIDFVCHSLGNLVVRRYL
                     GEANQPEPKWQVDKRIKRMVMLGPPNNGAHFAELFKDNTLFGVVAGPSGKQLAATWPQ
                     ASQRLAAPPAIEFGILAGGQGNSLGLNPLVPGDDDLLVSVAETRLPGARDFRLMHCRH
                     GAMMSDADVRQCILCFVEHGYFTAEDERQPIAALPLTPNPSPAKGEGGKAP"
BASE COUNT          698 a         1171 c         1303 g          687 t
ORIGIN      
        1 tcctgcccct tcgccgcccc ctcctccacc tccgccccgc tcaccaccgc cagcgtcccc
       61 gccacccgcg gcaactcccc atattcaatc cgccccggct ccccagcgat gccgggcagg
      121 ttcaacagct tcaccggctc cgcgccgaac gccgccgccg ctgccaacag gcaagccgcg
      181 gcaagccaaa tccgaagtaa cgaagggcgt cgtgagtcct gcatcaactt gtcccctccg
      241 gagattcaga actttgaaga gatcgtggac aacgacaggc tagcgcgtag ctctagaatg
      301 tagacttgat tctggagcaa gtgtctatgg ctgaagtcat tcacggccgc ttgaagtcga
      361 tgggggccaa aaacaaatgt cggtgcggca accggcactg gattgtctcc gaaacggatg
      421 acgcaagatt ctggtggctt tgcgtggcat gcggcgctcg ccatctgctc aacaagaagc
      481 aactccacgg gagattcccc aagcggatgg aaaagttcgc cgccccttcc tgatcgcttt
      541 cgttcaacgt cgcggcgggt ggccgcgtgt agctgtcagt cagcgctcca ccgccacagg
      601 gccggtagtg gcgacgcgag tgctgcttga cgccgggcga gcccgccatg aatggcgggc
      661 cgaaaaggca aagggccgtg aacggccccg ggggaggtcg tgccgtgcgg ccggccgatt
      721 catcctggcg cgggcggctc ggcggattca aggcggctgg acccgtcacg cgggcggccg
      781 gcggggcaat ttgtgaaatt ccgattagaa tgatgaaata aatatgtcca ctagcgcacc
      841 tataggaatg tggcagtgtg tgcgcggggt gggggtggct ggaggcgggc gagggaggga
      901 gcgggaagag gtgcagcaaa ggtgggaaag atgaggagag ttgggaaggg aggaaagagg
      961 cgggtgatgc gcgggtggaa gatcggacgg acggtgcagg tttttggcga gtcaaaaacg
     1021 cgcaacagcg caacatcctc atgcgggggg tgttgcacgg cgcaaagaat ttgctgtatg
     1081 ggagttaaga cgatgtcgcg cagccataga tcggtgcgaa aagaccgcga atgttgcgct
     1141 ccgcttccag cgctgctcaa agccggggcc tcagaaatgt tctgtcccac gcggaacctg
     1201 ggacggaacc gatggaccag gcttttacag gtgcgcggcc gagcgatcac gagtatcagc
     1261 attgctaata ttgcgatgct tgttttcgca tgccagggtt catcgccatg cctgaggtga
     1321 atttctggtc aattccgcgc gcccgctgac ttgacgcttg gggggacgga aaagaaaatg
     1381 taccggtgtg ccgaccggtg ggctttccgc ctctggctgg cgcatccctc ccgaagtcgc
     1441 tcgcccagac agatcacagg aaacggctga ttggtccacc cgaaagtggc ccagtggccg
     1501 ggatttgttg aactttccgg acaaattgcg actcccacct gcgaatagtg attgatcgga
     1561 ccagatttcg ctcgacctgc cgatgcaccg aatggccagc gaacagcccc gcgatgacgg
     1621 accgcgggca taccgctcag gggcggtttc cacctcaaag gagttgattc catgaataag
     1681 tccacgtccg ccgacgatgt caagctgatg agcagctacg agtcgctgcg gggcacactg
     1741 ctttccgata agtttcgcga ccgcctcgac cgccagctcg cgttttgggc tctgcccagc
     1801 gatcgacggc tgccgacggc gtttctgcac cgcaccctgg acgacctgct gtcttgctcc
     1861 tacaaggagt tagcctcgac cgcggggatc ggccacaaga agctgcaaac cttcgtcaag
     1921 ttgctggtgc gggcgacgaa ggaagacgcc agcaacacca cgctcgatga gatccacgcc
     1981 gcggaagagg agacaccgct ggcggggctc gattccgacg ggcagttcga tccggcgctc
     2041 gtttcggaaa tggtctggat tcagtggcgc gagacggccc gccgccacgg catcggccac
     2101 gagctgctgg ggcgtctgac tggcactttg cagtcgctcc cgtcggtcgt gtggaacaca
     2161 ccgctgtcct cctatctcga cttgtcgctg gccgaaatgc gggacctgaa gacgcacggc
     2221 gaaaagcgga tcggcgtggt gctcgaagtc tttcacaaca tccacaagat gctgggccag
     2281 gccgatcccg ccggcgggtt ggccgtgcgc ctcgccccgc ggaccatcgc ctcggcagag
     2341 gactggatcg ccgaagtccg cagtcgttcg tttccgccgg ggcacgagga gattgggcag
     2401 tatctcatcg agccgatcct gcagcagctc gcgatcgacg ccggcgacac ggtcgccaag
     2461 ctggcccgcg gacgcttggg cgtgggagcg ggaacggaat cggtccggca tcagagccaa
     2521 tcgctgggcg tgacccgggc ccgcgtctat cagatgctcg aagaatgtca tggcgtgatg
     2581 cagatccgct ggcccgaagg ccgccgccag ctcgacgact tcggccagtg gctcgacgag
     2641 tgctatgcct ccgccgaagc cgccaatctc gtcgccagct tccgcgagtt gctctacccg
     2701 ctcaagttcg actccgtcgg cgaagccctg cgggccgagg cggtgtgacg cttttgagct
     2761 tgctggctac agcgctcccg gctcggctgg ggtgcgagag gattcgaggt aggcggcgag
     2821 caggatttgc gcggcgagct tgtcgagccg ctccttgcgc tgcttcttgg tgaggccggc
     2881 gtcttggagg agcgattcgg cgtgggccgt ggtgaaccgc tcgtcgaaga gtcgcacggg
     2941 cagattggtc gtttcggaca gccacgcggc gaagcggcgg gcctcggctg atttctggct
     3001 ttccccgccg gtggtgtgca ccggcaagcc cacgaccagg ccggcgatgc gctcgcttga
     3061 cacgagctgc gtgatccacg ctgcgtccgc ggcgggattg cggcgggtgt agatttccag
     3121 cgggctagcc aggcgctgct ccggatcggt gatcgccacg ccgatccgca ccgtgccgta
     3181 gtcgatggcc gccagccgtc cctgccgcgg gagggcgtcg ctcatggcgc cttgcccccc
     3241 tcgcccttgg cgggagaggg gttgggggtg agggggagag cggcgatcgg ctgccgctcg
     3301 tcttccgcgg tgaaatagcc gtgctcgaca aagcacagga tgcactggcg tacgtcggcg
     3361 tcgctcatca tcgcgccgtg gcggcagtgc atcaggcgaa aatcgcgagc gccaggcagc
     3421 cgggtttcgg ccaccgaaac gagcaaatcg tcgtcgcccg ggaccagcgg attgaggccg
     3481 agtgaattgc cctggccgcc ggcgagaatg ccgaactcga tcgcaggcgg cgcggcgagc
     3541 cgctggctgg cctgcggcca ggtggcggca agctgcttgc cgctgggacc ggcgacgacg
     3601 ccgaagagcg tattgtcctt gaagagctcg gcgaagtgcg ccccgttgtt cggcgggccg
     3661 agcatcacca tccgtttgat ccgtttatcg acctgccact tcggctcggg ttgattggct
     3721 tcgcccaggt agcgccgcac gacgaggttg cccaggctgt ggcagacgaa atcgatccgt
     3781 tcggcatcgt tcagattgtc gatcacgcgg gccagcgagc gggcgtggtc gtcgagcgac
     3841 cggcgtgtgc tggcatagc
//
LOCUS       DASUGB010000268         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1560081, whole genome shotgun sequence.
ACCESSION   DASUGB010000268 DASUGB010000000
VERSION     DASUGB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560081"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1531)
                     /gene="odhB"
                     /locus_tag="VFV87_05170"
     CDS             complement(356..1531)
                     /gene="odhB"
                     /locus_tag="VFV87_05170"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5103177.1"
                     /translation="MATNVEVPSLGESVTEAILTQWLKAEGDEVAADEPICELESDKA
                     TVDLPAPAAGVLHRVKAAGETARVGEVIAEIKPLSGSDNQPQPAKQKTEQPENREIPK
                     KNDEYQRKAKKQEQPKEQPPPGPEAPAEEAAEPAAAERAIEKKPTQRRSSPPPSEAAE
                     GIRRVPMSKIRRSIADRLVRAQQTAAMLTTFNEIDMTAVQNVRKKQGERFHEAHGVSL
                     GLTSFFARACALALKQFPNVNAQIDNGDILFHDYVNLGLAVSTERGLIVPVLRHVESL
                     SLAQIESEIKRAAAAARDGKLSISDLSGGTFTITNGGIFGSLLSTPLLNPPQSGILGL
                     HAIQERPVAISGQVEVRPMMYVALTYDHRLVDGRESVSFLVRVKQLIEDPLQIILEI"
     gene            complement(1580..4231)
                     /locus_tag="VFV87_05175"
     CDS             complement(1580..4231)
                     /locus_tag="VFV87_05175"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5103178.1"
                     /translation="MSQATSADHRLAEGVADLIHSYRELGHCNARLDPLGHDRPPNPL
                     LELSEFGLSDDDLDVPVTAASYLGPPPGTLRKLIQQLQTTYCGTVGVEYMEIADKAQR
                     QWLQERMEPMLNQPELSADQSRRILKLLVAADTFEKFLHARFLGQKRFSIEGAEALVP
                     LLDTLIEDGADLGVEEVVLGMAHRGRLNVLAQVVHKPLEIILSEFLSKEAPHAHKEDE
                     GDVKYHLGYSSNRTTATGQQVHISLSNNPSHLELIDPVIEGLVRAKQERRGEEGRRLV
                     VPILIHGEAAFTGQGVVPETLNLSELPGYRTGGTIHIIVNNQLGFTATARETRFTPYP
                     TDVAKMIQAPIFHVNADNPEAVVHAARLAIAFRQRFQVDVMIDLVCYRRHGHNEADEP
                     AFTQPVMYREIDKHPPVAELYAEQLARRDRIDPDVPEQMSRQSRERLEEALRVAREFK
                     PKQRVFTLGGLWEGLSRAGDDWSAETAVAADVLATITQRMTQMPQGFQLHRKLRRSQE
                     ARAAMARGEKPIDWSCAEAWAIGSLLLEGTPVRLTGEDTERGTFSHRHAVLHDERTGE
                     PYVPLQHLAEDQAAFTVINTMLSELGVLGFEYGYSSADPWTLVIWEAQFGDFSNGAQP
                     IIDQFIASAEAKWQRMSGIVLLLPHAYEGQGPEHSSARLERFLQLCAEDNLQVCYPTR
                     SAQYFHLLRRQMRRNFRKPLIVMSPKSLLRDERAASALAEFSDGTFKPVIDDRAHPDR
                     EQARRLLLCSGRIYFTLEAARREHSLNDVALARVEQLYPLPRTELKTLIAHYPNLEEI
                     AWVQEEPRNMGAWTFIEPQLRDLLPEGCELVGHSRPAAASPATGSFRTHQAEELALVE
                     RALGRADAAKQPERGRESIAALAEQNG"
BASE COUNT          688 a         1389 c         1363 g          851 t
ORIGIN      
        1 gccaaagtgt cgtacgccgc taacacgcat tggtcatcca tggctaaacc ctttggccgt
       61 ctctgagcgc cgtaccgcca cccattcaaa cccgaaacgt gcaattaatg cgccaatcgt
      121 aggaggaaac atgcatgtca ctgcaatcga tcgatttggc gcttcaaatc cgcccacgcg
      181 acgaggcttc gtcactttgc acctgccatt ctgccggcgg ttggccggcg taattgggca
      241 ccagcgcgac catgagcagc acccctaggt cgatctcatt ctgaaacgcg ggcgaatgga
      301 gcctgccgcc gggcggagga accggcgcgg aatcgagcag ccaaggtttg ctgactcaga
      361 tttccaagat gatttgcagc gggtcctcga taagctgctt gacgcggacc aggaacgaga
      421 ccgattctct gccgtccacg aggcggtggt cgtacgtgag ggcgacgtac atcatgggac
      481 ggacttcgac ctggccgctg attgcgacgg gccgctcctg aatggcgtgc aggccgagga
      541 tgcctgactg cggcggattc aagagcggcg tggagagcag cgaaccgaat atgccgccgt
      601 tggtgatcgt gaaggtgccg ccgctcaggt ctgaaatcga gagcttgccg tcgcgcgccg
      661 ccgctgccgc ccgcttgatc tcgctttcga tctgggccag cgacaggctc tcgacgtgcc
      721 gcaggacggg gacgatcaat cctcgctccg tgctgacggc aaggcccagg ttcacgtaat
      781 cgtggaacag aatgtcgccg ttgtcgatct gggcgttgac gttgggaaac tgtttgaggg
      841 ccagcgcgca ggcgcgggcg aaaaacgatg tcagcccgag cgagacgccg tgcgcctcgt
      901 ggaaacgctc accttgcttc ttgcggacat tttgcaccgc ggtcatgtcg atttcgttga
      961 acgtcgtcag catggctgcc gtctgctgcg cccgcaccag ccgatccgcg atgctgcggc
     1021 ggattttgct catcggcacg cggcggatgc cttcggcggc ttccgagggc ggcgggctgg
     1081 atcgcctttg agtaggcttc ttctcgatgg ctctttcggc agcggccggc tcggctgctt
     1141 cttccgcagg cgcttccggc ccagggggcg gctgctcttt cggctgctcc tgcttcttcg
     1201 ccttgcgctg gtactcatcg tttttcttgg ggatctcccg gttctccggt tgctccgtct
     1261 tttgcttggc cggttgaggc tggttgtccg agccgcttag cggcttgatc tcggcgatca
     1321 cttcgcccac ccgggccgtt tcgccggccg ctttgacgcg gtgcagcacg ccggcagcgg
     1381 gcgcgggaag atcgaccgtg gccttgtccg attccagctc gcaaatcggc tcatcggcgg
     1441 cgacctcgtc cccctccgcc ttgagccatt gcgtaaggat ggcttcggta accgattcgc
     1501 ccaggctggg gacttcgaca ttagtggcca tctgttgcta ctccgccatt gcggggtcgg
     1561 gagtcgattg cggtcacggt catccattct gctcagcgag tgccgcaatc gactcccgac
     1621 ccctttcagg ttgcttcgcc gcgtcggctc gccccagcgc ccgctcgacg agcgccagct
     1681 cctccgcctg gtgcgtccga aacgagcccg tcgccggact ggccgctgcc gggcggctgt
     1741 ggccgaccag ctcgcagccc tcgggcagca aatcgcgcag ctgcggctcg atgaacgtcc
     1801 aagcccccat gttccgcggc tcttcctgaa cccacgcgat ttcctccaag ttcggatagt
     1861 gcgcgatcag tgtctttagc tccgtgcgcg gaagtggata gagctgctcg acgcgcgcca
     1921 gcgccacgtc gttcagcgag tgctcgcggc gggctgcttc cagcgtgaag taaatccgcc
     1981 cgctgcacag cagcaatcgc cgcgcttgct cgcgatccgg gtgcgcccga tcatcgatca
     2041 ccggcttaaa tgtcccgtcg ctgaactcag ccagcgcgga ggcggcccgc tcgtcgcgca
     2101 gcagactctt ggggctcatc acgatcagcg gcttgcggaa gttgcggcgc atctgccgcc
     2161 gcagcaggtg aaaatattgt gccgagcgcg tgggatagca aacctgcagg ttgtcttccg
     2221 cacaaagctg caggaaccgc tccagacggg cgctggaatg ctcgggaccc tgcccctcgt
     2281 aagcgtgcgg caagagcagc acgatgccgc tcatccgctg ccacttggct tccgcgctgg
     2341 cgatgaactg gtcgatgatc ggctgggcgc cgttggaaaa atcgccgaac tgggcctccc
     2401 agatgacgag cgtccacgga tcggccgagc tgtagccgta ttcgaaaccc aagacgccca
     2461 gctccgagag catcgtattg atgacggtga acgccgcctg gtcctcggcg aggtgctgta
     2521 gcgggacgta gggctcgccc gtgcgctcgt cgtgcaaaac cgcatgccga tggctgaacg
     2581 tgccgcgctc ggtatcctcg cccgtcaatc gcaccggcgt gccttcgagg agcaggctgc
     2641 cgatggccca cgcctcggcg cagctccaat cgatcggctt ttcgccgcgg gccatggcgg
     2701 cgcgcgcttc ctggctccgc cgcagcttgc ggtgcagttg gaagccctgc ggcatctgcg
     2761 tcatgcgctg tgtgatcgtt gccaacacgt cggctgcgac ggcggtttcg gcgctccagt
     2821 cgtctcccgc ccggctgagt ccctcccaga ggccgccgag cgtgaaaacg cgctgtttgg
     2881 gcttgaattc gcgggctacc ctgagcgcct cctccagccg ctcgcgggat tgccggctca
     2941 tttgctcggg cacgtccgga tcgattcgat cccgcctcgc aagttgctct gcatagagct
     3001 cggcgaccgg cggatgcttg tcgatttcgc ggtacatcac gggctgcgtg aaggcgggct
     3061 cgtcggcttc gttgtggccg tgccggcgat agcaaaccag atcgatcatc acatcgacct
     3121 ggaaccgctg ccggaatgcg atcgccaggc gggcggcgtg gaccaccgct tcgggattgt
     3181 cggcgttgac gtgaaagatc ggcgcctgga tcatcttcgc tacatcggtc ggatagggcg
     3241 tgaaccgcgt ctcgcgcgca gtcgccgtga agccaagctg attgttaacg atgatgtgaa
     3301 tcgttccgcc ggtgcggtag ccgggcagct cagacagatt gagcgtctcg ggcacgacgc
     3361 cttggccggt gaaggccgcc tcgccgtgaa tcagaatcgg cacgaccagg cggcggcctt
     3421 cctcgccgcg gcgctcttgc ttggctcgca ccagaccctc gatcaccggg tcgatcagct
     3481 ccagatggct cgggttgttg ctgagcgaaa tgtgcacctg ctggccggta gcggtcgtgc
     3541 ggttgctcga ataacccagg tgatatttga catccccctc gtcctccttg tgcgcgtgcg
     3601 gagcctcttt tgagaggaac tcgctgagga tgatctccag cggcttgtgc accacctgag
     3661 ccagcacatt cagccggccg cggtgggcca tgccaaggac gacctcctcc accccgaggt
     3721 ccgcgccgtc ctcgatcagc gtgtcgagca gcggcacaag cgcctcggcc ccttcgatgg
     3781 aaaaccgctt ttggccgaga aacctggcgt gcaggaattt ttcaaacgtg tccgcggcga
     3841 cgagtagctt caggatgcgg cgcgactggt ccgcgctgag ttctggctgg ttgagcatcg
     3901 gctccatccg ctcctgcagc cactgccgct gggccttgtc ggcgatctcc atgtactcaa
     3961 cgcccacggt gccgcaatac gtcgtctgaa gctgctgaat cagcttgcga agcgtcccgg
     4021 gcggcgggcc gagatagctg gcagcggtca ccggcacatc gagatcgtcg tcagacaggc
     4081 caaactcgct caattccaat agcggattgg gcggccggtc gtgccccagt ggatcgagcc
     4141 gggcgttgca atgccccagc tcgcgataag agtggatgag atctgccacg ccctccgcca
     4201 gccgatggtc cgcggacgtc gcctgcgaca cagtggttgc cctttgcgcg gccggcgaca
     4261 attctgctcg cggctgacca ctttggcccg c
//
LOCUS       DASUGB010000269         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_169318, whole genome shotgun sequence.
ACCESSION   DASUGB010000269 DASUGB010000000
VERSION     DASUGB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169318"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VFV87_05180"
     CDS             complement(<1..1499)
                     /locus_tag="VFV87_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103179.1"
                     /translation="MNDHFDITAQRQERLIADLKSLAAWRAGEEAEIAKRHAARDAEA
                     RRDHQRKQTAAAAKFQAAHARLIAEYHERREAVLCRYEAQGLVLAQEEDRLLAVHDQR
                     HGESLDAAKTLRQHRVAGIQREFREQKLIPRADLAKFQEHCEESSQELTALTVRTQAA
                     VRRRAAWPEEAAPPPEAPAGLSRPQAVEQFSAALADAYARSQELTGVASARFLDEGWP
                     VLIFLALLAAFGYPAYRLVAVYGLLLVAIGTVLAALAITGIAFAIVVPLARRALLRRV
                     PPFQEAAARARVYLAAALAAARIEAARRHRRIVEHASRELAAAKAESKQSRRELKALR
                     ATRITQTHAEYAGRRRIVEETHERELDQIEDKHPRRIEALEQDFTRELEAIDDQLRRA
                     FAASRRQREGEVEQVQSRWRAGLEDFRAAAQAMNEYCQAVAPPWDQVAERLAGGQSAA
                     RLETVTALPIGRLRIELPTLSGEAPAPPLPAALVYPDQPSLLIKAADAGR"
     gene            complement(1642..1881)
                     /locus_tag="VFV87_05185"
     CDS             complement(1642..1881)
                     /locus_tag="VFV87_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103180.1"
                     /translation="MREADLTSGLGQLTQAFAKLQERWTQAQEHWNDDAGRRFGQSCL
                     QPLPARLKLIAAAAGSLQRTIAGAQRELGDEAGEA"
BASE COUNT          289 a          697 c          675 g          343 t
ORIGIN      
        1 cggccggcgt cggcggcttt gatgagcagc gagggctgat cgggatagac cagcgccgcg
       61 ggcagcggcg gcgcgggagc ttcgcccgag agcgtcggca attcgatcct cagccgcccg
      121 atcggcaggg ccgtgaccgt ctcgagcctg gcggcggact gcccgccggc caaccgctcg
      181 gcgacctggt cccagggagg cgcaacggcc tggcagtact cgttcatcgc ctgggcggcg
      241 gcgcggaagt cctccaggcc ggcccgccac cgcgactgaa cctgttccac ctcgccttcg
      301 cgctggcgcc ggctggccgc aaaggcccgc cgcagctggt cgtcgattgc ttccagctcg
      361 cgggtaaagt cttgctccag ggcctcgatc cgccgtgggt gcttgtcctc aatctggtcg
      421 agctcgcgct cgtgcgtctc ctcgacgatc ctgcggcggc cggcgtactc ggcatgcgtt
      481 tgagtgatcc gcgtggcccg cagcgccttc agctcacggc gcgactgctt ggattccgcc
      541 ttggcggcgg ccagttcccg gctggcgtgc tcgacgatcc gccggtgccg ccgggccgcc
      601 tcgatgcgag cggctgcgag cgccgcggcc agatagacgc gggctcgggc ggccgcttcc
      661 tgaaacgggg gcacccgccg caacagcgct cgccgggcca gcggtacgac gattgcaaac
      721 gcgatgcccg taattgccaa ggccgccagc accgttccaa tggcgacaag tagcaggccg
      781 taaaccgcga ccagccgata cgctggatag ccaaatgcgg cgagcagagc caggaagatc
      841 agcaccggcc agccctcgtc gagaaaacgg gcgctggcca cgccggtcaa ctcctgcgac
      901 cgggcgtaag cgtcggccag tgcggccgag aactgctcga ctgcttgcgg gcgcgacaag
      961 ccagccgggg cttcaggcgg tggagcggct tcctcgggcc aggcggcgcg acggcggacg
     1021 gccgcctgcg tccgcacggt cagggcggtc agctcttgcg agctttcctc gcagtgctct
     1081 tgaaacttcg ccagatccgc ccgggggatg agcttctgct cgcggaattc ccgctggatg
     1141 cccgccacgc gatgctgccg caacgtcttg gccgcgtcga gcgactcgcc atgccgttgg
     1201 tcgtgaaccg ccagcagccg gtcttcttcc tgggcgagca ccaagccttg ggcctcgtag
     1261 cggcacagga ccgcctcgcg acgctcgtgg tattcggcga tcagccgggc atgggcggct
     1321 tggaactttg ccgctgcggc cgtttgcttt cgctggtgat cgcggcgggc ctcggcgtcg
     1381 cgcgcggcgt gccgcttggc gatctcggcc tcctcgcctg ctcgccaggc agccaacgat
     1441 ttgagatcgg cgatgagccg ctcttgccgc tgggccgtaa tgtcgaagtg gtcattcacg
     1501 gacaggccaa ttcgttcgta aaagcgcccc tcacatcaat ccccgcatag gaagctccac
     1561 aggaagctgc cacaggaagc ctgaatcgca agcgaaggat ttttcgtttc cgatttcgca
     1621 ccttcgcttg cgcgtccggc tttatgcttc ccctgcttcg tcgcccaatt cccgctgggc
     1681 cccggcaatg gtccgctgca agctgccggc cgccgcagcg atcagcttga ggcgcgcggg
     1741 cagcggctgg aggcacgatt ggccgaagcg gcgtccggca tcgtcgttcc agtgctcctg
     1801 cgcctgggtc caccgctcct gcagcttggc aaaggcctgc gtcaattgcc caagtccgct
     1861 ggtcagatca gcttcccgca tcggcggctc ctttcgcact gtcgtccata gcggctggcg
     1921 gtccgccggc ggcgacatac cggtccaggg cgtcgagcat ccggcccagg gcggcaatcg
     1981 cttgggccag gtcggtgtcc aaat
//
LOCUS       DASUGB010000270         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1560279, whole genome shotgun sequence.
ACCESSION   DASUGB010000270 DASUGB010000000
VERSION     DASUGB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560279"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1298
                     /locus_tag="VFV87_05190"
     CDS             195..1298
                     /locus_tag="VFV87_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5103181.1"
                     /translation="MKKYRVGIIGHTGRGNYGHGIDTVWRHFPDECEVVAVADADEKG
                     RAEAAVRTKAPKAYADYRQMLDEAKPEIVAIGPRWLDQHRDMVLAAAERGAHVYMEKP
                     MCRTPAEADQMALACEKHDVKLALAHQTRYSPRLQPVHDLIADGQLGTILELRGRGKE
                     DARGGSEDLWVLGSHVFNLINHFGGSPTWCFARVLQNGKPVSKADVREGNEGIGPLAG
                     DQVTAMYGLDSGATAYFGSQKSMGGGNGRFGVQIFGSKGIVEILTGHLPSVQFLPDPA
                     WSPGRSGAKWIPVTSAGVGKPEPLGDGGLDGGNILAVKDLLAAIEQDRQPECSVYEGR
                     TIIEMICAVFESHRVGGPVNFPLKSRENPLTLL"
     gene            1411..>2091
                     /locus_tag="VFV87_05195"
     CDS             1411..>2091
                     /locus_tag="VFV87_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5103182.1"
                     /translation="MHLHFLGAARQVTGSRYVLEAAGRRVMIDCGMFQERNFQERNWA
                     PAPIPAREIEALLLTHVHIDHCGLIPKLVSEGFRGPIHATEPSAALLEIMLRDAAEIQ
                     QEDIGYKKRRHAKEGRKSPHPYEPLFTEADVDQTLPLVRPCKYGEAVQVTPEITARYF
                     DAGHILGSAMIEVDAVESGKTTRIAFSGDIGQPNKPIIRDPTLLLHADYVVMESTYGD
                     RNHQRPGDT"
BASE COUNT          410 a          652 c          670 g          359 t
ORIGIN      
        1 cccaaagtcg attctgcgcc gtccggcgcg tggcaatcac ctccctggcg tgttacaacg
       61 caacggcctg cattcacaat aacccgaagc gtaagtgagg gacgcgcctc gctaatgctt
      121 cgggttacgg cgaggaactc ctcgcttacg catcgggttg cgatgagccg ctatccacac
      181 aagggagcct cgccatgaag aaataccgcg tcggcatcat tggacatacc ggccgtggca
      241 attatgggca tgggatcgac acggtctggc ggcattttcc agacgagtgc gaagtggttg
      301 ccgtggccga tgccgacgag aagggccggg ccgaagcagc ggtccgcacc aaggcgccca
      361 aggcgtatgc ggattaccgc cagatgctcg atgaggctaa accggagatc gtcgccatcg
      421 gcccgcggtg gctcgaccag caccgcgata tggtcttggc cgctgccgag cggggcgccc
      481 acgtctatat ggaaaaaccg atgtgccgca cacccgcgga agcggaccaa atggcccttg
      541 cctgcgaaaa gcacgacgtg aagctggcgc tggcccatca aacccgctac agcccgcgcc
      601 tgcagccggt acacgatctc atcgccgacg ggcaactcgg cacgattctg gaattgcgcg
      661 gccggggcaa ggaagatgcc cgcggcggga gcgaggatct gtgggtgctg gggagccacg
      721 ttttcaattt gattaaccat ttcggcggct cgccgacctg gtgcttcgcc cgcgtgctgc
      781 aaaacggcaa gcctgtgagc aaggcggacg tgcgagaagg gaacgagggg atcggcccgc
      841 tcgccggcga tcaggtgacc gcgatgtacg gactggatag cggggcgacc gcctacttcg
      901 gctcgcagaa atcgatgggg ggcggcaacg gccggttcgg agtccagatc ttcggcagca
      961 agggaatcgt ggagatcctg acggggcatt tgccgagcgt ccaattcctg cccgatccgg
     1021 cgtggtcgcc ggggcgctcg ggggccaaat ggattccggt taccagtgcg ggagtgggca
     1081 agcctgagcc gctcggtgac ggcgggctcg acggggggaa tattctggcg gtgaaggacc
     1141 tgttggcggc catcgaacag gaccgccagc cggagtgcag cgtgtatgaa ggccgcacga
     1201 tcatcgagat gatctgtgcg gtgtttgagt cgcatcgcgt cggcggcccg gtgaacttcc
     1261 cgctcaagtc gcgggagaat ccgctaacgc tgctgtagaa gaaccaagaa ccaaagaaac
     1321 aagaaccaag tctcccgcaa cctggttctt cggtccttgt ctcttggttc ttcccttggt
     1381 tcttcggttc ttgtttcttg gatcttcccc atgcacctcc acttcctcgg cgccgcccgc
     1441 caggtcaccg gcagccgtta tgtgctggag gctgccggcc ggcgggtgat gatcgactgc
     1501 gggatgttcc aggagcggaa tttccaggag cggaactggg cgcctgcgcc gatccccgct
     1561 cgcgagatcg aggccctgct gctgacgcac gtgcacatcg accactgcgg gctgattccc
     1621 aagctcgtca gcgaaggctt ccgcgggccg atccacgcca ccgagccctc cgccgccctg
     1681 ctcgagatca tgctccgcga cgccgccgag attcaacagg aagacatcgg ctacaagaag
     1741 cggcggcacg cgaaggaagg acgcaaaagc ccgcaccctt acgagccgct ctttaccgag
     1801 gccgacgtcg atcaaactct cccgctcgtt cggccgtgca aatacggcga ggcagtgcag
     1861 gtgacacccg agatcaccgc ccgctatttc gatgccgggc atatcctcgg ctcggccatg
     1921 atcgaggtcg atgcggtcga aagcggcaaa acgacgcgga tcgcgttttc cggcgacatc
     1981 ggccagccca acaagccgat cattcgcgat ccgaccctgc tgttgcacgc cgactatgtc
     2041 gtgatggaat cgacttacgg cgaccgtaac caccagcggc ccggcgacac c
//
LOCUS       DASUGB010000271         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_308700, whole genome shotgun sequence.
ACCESSION   DASUGB010000271 DASUGB010000000
VERSION     DASUGB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308700"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VFV87_05200"
     CDS             <1..1070
                     /locus_tag="VFV87_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103183.1"
                     /translation="FRPGVRWIPTYRIALAAEKAKKTADIALQAELLNEAEDLKDVPL
                     DIVVGVPNFRFRETPSPLVLESVLRNALAQTDPHLMGQASNSLSNAMYTQRSSEFRRN
                     AAMANAEGQEGQLNLPGELTASGAQDLFVYGLPKLSVAKSERAAVSIFTAEVPYRDIH
                     TWDVHVARHDIEAAPSGAGVNSPLTLSKNEVWHQIELTNATNLPWTTGAAMIMQGQQP
                     LSQELLTYTPPKNDVRVPVTVSVDTRGSFDEQETGRELKALNWDGYNYARITKEAKLH
                     LCNNKPVAITIEITLRLGGKVDAASDMGRVTLSAFNQADWIQYHGHPAVNNSSVVTWK
                     VKLEPGETFEPKVQYHYFARH"
     gene            complement(1084..1821)
                     /locus_tag="VFV87_05205"
     CDS             complement(1084..1821)
                     /locus_tag="VFV87_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103184.1"
                     /translation="MKGANDLIARVARLCPNRLASIDNPYAHLDGRYEVELIDLVKES
                     DDYDSAGLLCWMPNVGTFGCVDEEHSTVLSFPDSTWPDIVRHPVEHLDSQWGLGSVPV
                     EETLPWLWFDFRLKDRDLVLSPYPDACPFHQEALTIDHSPSPVLLRVYRRREPTAWLD
                     NHVGTFPCSGVPVSERETKCCSRCRNLELDWMGAVANAIPEADAKLNSQGWIQCPGCG
                     VRFSTMDEARFQFGTHLACGQRIRLIS"
     gene            2049..>2763
                     /locus_tag="VFV87_05210"
     CDS             2049..>2763
                     /locus_tag="VFV87_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103185.1"
                     /translation="MYKLLLSWRYLRTRYIALASIVSVTLGVGTLIVVNSVMAGFARE
                     MHQRLHDILADVIVESHSLDGLPNPQWHMEEIRKVVGDDLVSMTAAVHVPAMLNIQVR
                     GEWITRQLTLIGIDEATYADVSDFRPFLLHPGNRENLSFRLREEGYGNDDHELPPSGW
                     KYRRLKVAYEREYEAQQRLLRDAMDPSGAAGQESGVRGQGSEDRGQKTEDSESAIRNP
                     QSAITSESAIPSDPFASASA"
BASE COUNT          586 a          897 c          794 g          486 t
ORIGIN      
        1 atttccgccc cggcgtgcga tggattccga cctaccgcat cgcgctggct gccgagaagg
       61 cgaaaaagac ggccgatatc gccctgcaag ccgagctgct caacgaggcg gaggacttga
      121 aggacgtgcc gctcgacatc gtcgtcggtg tgccgaactt tcgctttcgc gagacaccca
      181 gcccgctggt gctcgaaagc gtgctccgca acgccttggc ccagaccgat ccgcacctga
      241 tggggcaggc cagcaacagc ctttccaacg cgatgtacac gcagcgctcg agcgagttcc
      301 gtcgcaatgc cgcgatggcc aatgctgagg gccaggaagg tcagctcaac ctgcccggtg
      361 aattgaccgc gtccggcgct caggacctgt tcgtctatgg cctgccaaag ctgagcgttg
      421 ccaagagcga gcgggccgcc gtctcgatct ttactgccga ggtgccgtat cgcgacatcc
      481 acacctggga cgtccacgtc gcccggcacg acatcgaagc ggctcccagc ggcgcggggg
      541 tcaattcgcc gctcacgctc tccaagaacg aggtctggca ccagatcgag ctgaccaacg
      601 ccaccaacct gccctggacc accggcgccg cgatgatcat gcagggccag cagccgctct
      661 cgcaggagct gctgacctac acgccgccga agaacgacgt ccgcgtgccg gtcaccgtct
      721 cggtcgatac gcgcgggagc ttcgacgagc aggagaccgg ccgcgagctc aaggcactca
      781 actgggacgg ctacaactac gcccgcatca ccaaggaagc caagctgcac ctctgcaaca
      841 acaagccggt cgcgatcacg atcgagatca cgctgcggct ggggggcaag gtcgatgcgg
      901 cgtcggacat gggccgcgtc acgctctccg ccttcaacca ggccgactgg atccagtatc
      961 acggccatcc cgccgtcaac aacagctcgg tcgtcacctg gaaggtcaag ctcgagcccg
     1021 gcgagacctt cgagccgaag gtgcagtatc actactttgc gaggcattaa aaaccatcgt
     1081 cgatcacgag atgagccgaa ttctctgtcc gcaggccaag tgtgtgccga attgaaatcg
     1141 agcctcgtcc atagttgaga acctgacgcc acagcccggg cactggatcc atccctgcga
     1201 attgagcttc gcatccgctt ctggaattgc gttcgcgacc gcgcccatcc aatcgagctc
     1261 aagattacga caacggctac agcattttgt ctcgcgttca ctcactggca ctcctgaaca
     1321 tgggaatgtg ccgacatggt tatcgagcca ggccgtgggt tctcgcctgc ggtaaacgcg
     1381 cagcaggact ggactcggcg aatggtcgat tgtgagcgct tcctgatgga atgggcatgc
     1441 gtcagggtac ggactcagaa ccaagtcgcg atccttcaat cggaaatcga accacagcca
     1501 cggcaatgtc tcctcaaccg gcacgctgcc cagaccccac tgtgaatcga gatgttcgac
     1561 aggatgtcga acaatgtcgg gccaagtcga gtcgggaaag gaaagcaccg tggaatgttc
     1621 ctcatcgacg cagccaaaag ttccgacgtt tggcatccag caaagcaatc ccgccgagtc
     1681 gtaatcatcg ctttccttga cgaggtcgat caactcgact tcgtagcggc cgtccaaatg
     1741 cgcatacggg ttatcaatcg aggccagacg gttaggacaa agcctcgcca ctctcgcaat
     1801 cagatcgttc gcacccttca atgtgatacg cccgatcgaa gacccagcag cacgaaagcc
     1861 aagctgctgc cccgaccgga ggaattgcaa gcagtcgtca ggcagccctt tcattgcctt
     1921 ccccgtgatt agctagcatc aattgggatt ccgccctcgc cacgttcgat ttataactcc
     1981 ccctcccgga ttgccgccaa ccattgctgg cattcgattt tcgcccgcct gccggccaag
     2041 gacggcctat gtacaagctg ctgctttcgt ggcggtatct ccgcacccgc tacatcgccc
     2101 tggcctcgat cgtcagcgtc accctcggcg tcgggacgct gatcgtcgtc aacagcgtta
     2161 tggccggctt cgcccgcgag atgcaccagc gactgcacga tattctcgcc gacgtgatcg
     2221 tcgaatcgca tagcttggac ggcctgccga atccgcagtg gcacatggag gagatccgca
     2281 aggtcgtcgg cgacgatctg gtgagcatga ccgcggccgt ccacgtgccg gcgatgctca
     2341 acatccaggt tcgcggcgag tggattacgc ggcagctcac gctgatcggc atcgacgaag
     2401 cgacgtatgc cgacgtgagc gacttccgcc cgttcctcct gcatcccggc aatcgcgaaa
     2461 acctgagctt ccgccttcgc gaagagggct atggcaatga cgatcatgag ttgcctccca
     2521 gcgggtggaa gtatcgccgc ctcaaggtgg cctacgagcg cgaatacgaa gcccagcagc
     2581 gcctgctgcg ggacgcgatg gacccgagtg gagcggcggg gcaggagtca ggggtcaggg
     2641 gtcaggggtc agaagacaga ggacagaaga cagaggacag cgaatccgca atccgcaatc
     2701 cgcaatccgc aatcaccagt gaatccgcaa ttcccagcga tcccttcgct tccgcctccg
     2761 ccg
//
LOCUS       DASUGB010000272         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1698220, whole genome shotgun sequence.
ACCESSION   DASUGB010000272 DASUGB010000000
VERSION     DASUGB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698220"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VFV87_05215"
     CDS             <1..1355
                     /locus_tag="VFV87_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103186.1"
                     /translation="LSLLGLASAQLERLRAQSPTPSETAQKPRAKSCVFLFLFGGPSQ
                     VDLWDMKPQAAPEIRGEFKPSATNVPGIHICEHLPKLAQQMDKLCLVRSMSHRMNVHG
                     PACSEVFTGREYFGPPTTDQATREDWPSLSSLVMRYGSSRGGLAPSVVLPWYLQFTGQ
                     ARRIAGQTGARMGEIHNAVLVSGDFGRSDYELQGLKLAEGVPWERMVQRRGLLKDIGF
                     GESSRGDLGPGLTMLDANRSRAWSLLESRAGEVLDLTREPAKVRERYGQTTVGQSLLM
                     ARRLVEAGVSLVTVNWQDETYTDGVNTCWDTHQNNFPKLKTLLCPLFDRAFPAFIADL
                     HERGLLEKTLVVALGEFGRTPRMGQFSQSANTQQTGRDHWPHAFTAILAGGGVRGGQV
                     YGATNENGGYVSDKPVSPADLTATILAHLGIDPALTYHDEFQKLDRKLSEGRIVAGLG
                     "
     gene            1432..1752
                     /gene="rpmB"
                     /locus_tag="VFV87_05220"
     CDS             1432..1752
                     /gene="rpmB"
                     /locus_tag="VFV87_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5103187.1"
                     /translation="MAQCEYCEKKPSMGNQVETRGKAKYLGGVGTKVTGISRREFKPN
                     LQRVKITTPGGTTRYARICTRCLKKGLVRKAVKQAPFKLRAAAGKGSQPAAAAAPKPA
                     KASK"
     gene            1865..2155
                     /gene="gatC"
                     /locus_tag="VFV87_05225"
     CDS             1865..2155
                     /gene="gatC"
                     /locus_tag="VFV87_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5103188.1"
                     /translation="MSLTRADVEKVSLLGRLLLSETELDTMTTQLTRVVGYVEQLREV
                     NTENVQPMAHAVEMHNVFRDDALGESLPRDAALANAPKRDSECYRVPAVLGE"
     gene            2166..2600
                     /locus_tag="VFV87_05230"
     CDS             2166..2600
                     /locus_tag="VFV87_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103189.1"
                     /translation="MARSVEQLIEDIKAYQPSGNENDDLDRLYAITESYESMGGLSRA
                     VPVIIDAFERFPEAWWGNPGPLVHLVEHCGGHVDLLIDSIRRTPTPTTVWMVNRILNA
                     AIHLDVRTKLLATLTFAANDTRTSEATRTAARSFLDHQSHRV"
BASE COUNT          500 a          841 c          846 g          447 t
ORIGIN      
        1 gcttgagctt gctggggctg gcgagcgcgc agctcgagcg gctgcgggcc cagtcgccga
       61 cgccgagcga gacggcgcaa aagccacggg ccaagtcgtg cgtcttcctg ttcctcttcg
      121 gcggaccgag ccaggtcgat ctgtgggaca tgaagccgca ggcagcgccg gagattcgcg
      181 gcgagttcaa acctagcgcg acaaacgtgc cgggcatcca catctgcgag cacctgccca
      241 agctcgctca gcagatggac aagctgtgcc tggtgcggtc gatgtcgcac cggatgaacg
      301 tccacggccc ggcttgcagc gaggtcttca ccggccgcga atacttcggc ccgccgacga
      361 ccgaccaggc cacgcgcgaa gactggccca gcctcagcag ccttgtcatg cgctacggct
      421 catcgcgcgg cggcctcgcg ccgtcggtcg tcctgccgtg gtacttgcag tttaccggcc
      481 aggccaggcg gatcgccggc cagaccggcg cccggatggg agagattcac aacgcggtgc
      541 tggtcagcgg cgatttcggc cgctctgact acgagctcca gggcctgaag ctcgctgaag
      601 gcgtcccctg ggagcgcatg gtccagcgcc ggggcctgct aaaggacatc ggcttcggcg
      661 agagcagccg cggcgacctg ggcccgggcc tgacgatgct cgacgccaac cgctctcgcg
      721 cctggtcgct gctcgaatcc cgggccggag aagtgctcga cctgacgcgc gagccagcga
      781 aggtccgcga gcgctacggg cagacgaccg tcggccagag cctgctgatg gcccggcggc
      841 tggtcgaggc gggcgtttcg ctggtcacgg tcaattggca ggacgaaacg tacaccgacg
      901 gcgtcaacac ctgctgggac acgcaccaga acaatttccc gaagctcaag acgctcctct
      961 gtccgctctt cgatcgggcg tttcccgcgt tcatcgcgga cctgcacgag cgaggccttt
     1021 tggaaaaaac gctcgtcgtt gccctcggcg aattcggccg cacgccgcgg atggggcaat
     1081 tctcgcaaag cgccaacacg cagcaaaccg gccgcgatca ctggccgcat gccttcacgg
     1141 cgattctggc cggcggcggc gtccgtggcg ggcaggtcta tggcgcgacg aacgagaacg
     1201 gcggctacgt gagcgataaa ccggtttcgc cggctgacct gacggcgaca atcctggctc
     1261 atctgggcat cgatcccgcc ctgacctacc acgacgagtt ccagaagctc gaccgcaagc
     1321 tgagcgaagg cagaatcgtc gccggtctgg gctagcggat tacgggcttc gtcgcggctt
     1381 tgcgcgccgg cggggggccg ctatattggt ggattcttcg gaggatttgc gatggctcag
     1441 tgcgaatatt gcgagaaaaa gccgagcatg gggaaccagg tggaaacccg cggcaaggcg
     1501 aagtatttgg gcggcgtcgg taccaaggtc accggcatct cgcggcggga gttcaagccg
     1561 aatctgcagc gggtaaagat caccacgccc gggggcacca cgcggtacgc ccggatttgc
     1621 acgcggtgcc tgaagaaggg gctcgtccgc aaggcggtca agcaggcccc gttcaagctc
     1681 cgcgccgcag ccggcaaggg ctcgcagccc gccgccgcag ccgcgcccaa gccagccaag
     1741 gccagcaaat aacaacaaag ccgcgaccat tggtcgcggt tcccattgca cccgcccggc
     1801 accgcgacca ctggtcgcgg ctttgtggat gggcggccac tggccgcggc tatggaggat
     1861 ttccatgtct ctcacccgcg ccgacgttga aaaggtctcg ctcctcggcc ggctgctcct
     1921 ctccgaaacc gagttggaca cgatgaccac gcagctcacg cgcgtcgtcg gctacgtcga
     1981 gcagctccgc gaggtgaaca ccgagaacgt gcagccgatg gcgcacgccg tcgagatgca
     2041 caacgtcttc cgcgacgacg cgctcggtga gtcgctgccg cgcgacgccg cgctcgcgaa
     2101 cgcccccaag cgcgacagcg aatgttaccg agtgccggca gtcttgggag agtgaggcgg
     2161 tgcccatggc gcgatcagta gagcagctga ttgaagacat caaggcgtat cagccgtctg
     2221 gaaacgagaa cgacgatcta gaccgccttt acgccattac tgaatcctac gaatccatgg
     2281 gaggactgtc tcgcgcggtc cccgtgataa ttgacgcctt cgaacgattt cccgaggcat
     2341 ggtggggcaa tccaggtcct ctggtgcatt tggtcgaaca ttgcggcggt catgtggatt
     2401 tattgattga ctcgatacgt cgaactccta cgccaacaac ggtttggatg gtcaatcgaa
     2461 ttctaaatgc ggcaatccac ctggatgttc gaaccaagct gctggctacg ctcacgttcg
     2521 ctgctaatga tactcgcacc tcagaggcaa ctcgaactgc tgcgaggtca tttctcgatc
     2581 atcaatcaca tcgggtttga ttcggacttg aggcagattt gaatacgcgc tctg
//
LOCUS       DASUGB010000273         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_308835, whole genome shotgun sequence.
ACCESSION   DASUGB010000273 DASUGB010000000
VERSION     DASUGB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308835"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFV87_05235"
     CDS             complement(<1..209)
                     /locus_tag="VFV87_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEX5103190.1"
                     /translation="MKFAQVYPLYVQKAERKNRTKAEVDQIIRWLTGYTEQGLQQQIA
                     KESDFATFFAEAPAMHPNRSLITGVV"
     gene            complement(439..726)
                     /locus_tag="VFV87_05240"
     CDS             complement(439..726)
                     /locus_tag="VFV87_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103191.1"
                     /translation="MNSLTEQLGKLYESGQVEVDEVIEARVQLFQAGLDAAETQSDRL
                     TLYKNIVKELKRYEEIAQARMEAGQGLATAVLKIQARRLKVEIQLAQAQAE"
     gene            complement(1112..1633)
                     /locus_tag="VFV87_05245"
     CDS             complement(1112..1633)
                     /locus_tag="VFV87_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103192.1"
                     /translation="MSYAVYCLAKTDAQAIRIANRLRAAGFSESEISILAPDRGPLRD
                     ASHENSTKAPEGAVTGAGTGALLGGALGWLVGVGALAIPGVGPLIAAGPILAALSGAA
                     LGGAAGGLTGALVGLGIPEYEAQAYEGKLREGNILIATHVENSEEAGSVRTIYNEEKA
                     ESISTGSEASVPS"
     gene            complement(1697..2131)
                     /locus_tag="VFV87_05250"
     CDS             complement(1697..2131)
                     /locus_tag="VFV87_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5103193.1"
                     /translation="MRITILGMLLAVALAGCEPIATDESPVPDAQAPGTQATNDPANQ
                     PAEPDNTAVNERDADGATKTPIDQDESQADVSTTAKIREQITSQEGMSINARNVKVMT
                     SNGRVTLRGPVDSSAERETIERIAQDVAGAANVDSQIEVINR"
BASE COUNT          534 a          857 c         1011 g          638 t
ORIGIN      
        1 acgacgccgg tgatcagcga acgattgggg tgcatggccg gcgcctccgc aaaaaacgtt
       61 gcgaagtcgc tttcctttgc gatctgctgc tgcaagccct gctcggtata gcccgtcaac
      121 cagcgaatga tctggtccac ttctgctttc gtacgattct tgcgttccgc tttctgaaca
      181 tagagcggat aaacctgggc gaatttcatc gcgaagactc gctgataatt cgtcgccgtt
      241 cgtccggacg gtcgcgggcc atccttgctc ttggcccgag cgggagccgc tttggccttc
      301 gcagcttggg gtctcgtcgc cgtcgtggac tttgattttt tagccatggc acgcattgga
      361 acaaatgtgc ggcgcttcgc cgcgatgctg ttgtactcgg gggccggcag ctaccctttc
      421 ctggtgcttc acgtcggatt attcagcctg tgcctgcgcc agttgaatct cgactttcag
      481 acgcctcgct tggatcttga ggacggcggt cgctaaccct tggccggcct ccattctggc
      541 ttgggcgatc tcttcgtaac gtttcagctc cttcacgatg ttcttgtaga gcgtgagtcg
      601 atccgactgc gtctccgccg cgtccagccc ggcctggaac aattgcaccc gcgcctcgat
      661 cacctcatcg acctcgacct gcccgctctc gtagagcttg ccgagttgct cggtgagcga
      721 attcaagatg gcgagccgct ccgcctgaag tcctttgact ttcgtggctg actctggtct
      781 ctgttgtgag ggaggcgggt ttgcctgatc ctgcgccccg gcaggagcga ttagcagcat
      841 agccatgacg gacgggatta gagcagatgt cctcatcgtg gacctccgag agcgggtaag
      901 agcgttctgt cccggcgcca gccacttttc gcttacagca gcgaatgcgc tgcggctgag
      961 ccgcccggtc attgtattcc gctgtggcgg agccgtggga tcaggacaac gcgcctggca
     1021 agaactgccg gagatcaagg gcggctggcg ggcgggtcct cgggtcgccg ccgcagaagg
     1081 cggcgaccca gattgttgcg aacgctgtcg ctcagctcgg aacggaggct tcgctgcccg
     1141 tcgagatgct ttccgccttt tcctcattgt agatcgtgcg gacgctgccg gcttcttcag
     1201 agttctcgac gtgggtcgcg atcaggatgt tgccttcgcg caacttgcct tcgtaggcct
     1261 gcgcttcgta ctcggggatg ccgagcccga cgagcgctcc cgtcagcccg ccggccgcgc
     1321 ctcccaaggc cgcgcccgac aaggctgcca aaatgggccc ggccgcaatg agcggaccca
     1381 cgccgggaat ggccagcgcg ccgacgccga ccagccagcc cagcgcaccg ccgagcaggg
     1441 ctccggtccc ggcgcctgtg acggcgcctt ccggcgcttt ggtcgagttc tcgtggctcg
     1501 catcccgcag ggggccgcgg tctggggcca aaatggagat ttcggactcc gagaagcccg
     1561 ccgctcgcag gcgattggcg atgcgaatcg cctgagcgtc ggtcttggca aggcaataaa
     1621 cggcataact catctggcag ttccttaatg cttgaatggg tttgcaaaca gactgatggg
     1681 cctgaattat cacggatcag cgattgatca cttcaatctg gctatcgacg ttcgctgcgc
     1741 cggcgacatc ttgcgcgatg cgctcgatcg tttcgcgttc ggccgatgaa tccacggggc
     1801 ctcggagcgt aacgcggccg ttggacgtca ttactttgac gttccgggcg ttgatcgaca
     1861 tgccttcctg cgaggtgatt tgttcgcgga tcttggccgt cgtcgagacg tcggcctggc
     1921 tttcatcctg atcgatcggc gtcttcgtgg cgccgtcggc gtcgcgctca ttgaccgccg
     1981 tattatcggg ctccgcaggc tggttggcgg ggtcgttggt cgcttgcgtg cctggggctt
     2041 gggcgtcggg aacgggcgac tcgtccgtcg cgatcggctc gcagcccgcc agagcaacgg
     2101 ccagcagcat gccgagtatg gtgattctca tttcggtctc cttgatgatt ggctggaacg
     2161 ccggcaacgc gttggtttgc cagcccttgc gtgggaattt gcatttcccg tgccaagggg
     2221 acggtattcg ttcagccgcc cgaaaatcgt gctggcggca ctggcgttag gccaggaagg
     2281 cgacacggca cggcaaagcg accgacgcag cggcagagtt gccagcgaga gaggtgggag
     2341 gcgttcgaat tcggccacgt cagggagttt ctggccgaga gcacggcggg cagcaggcgc
     2401 aggcccagct attgggtgcg ctggcgattt cctgaattcc ggggagcgca agggaataaa
     2461 cggagagatt tacgcatcta tacagtatta agtgttctta tgatgcgaga ccggaaaact
     2521 gagcggaggg gggagacttg gcaggtgagg gaagaggaga cagggagaga gtttcggaaa
     2581 tgttcaaaca ttgcagcgcg caacagcgca acatcctcga atgggggagt cgatgtgatg
     2641 caaggtactg agggacaagg agttgtggaa agtgttgcgc gagtgggcgc tgcgcgaaaa
     2701 agtgtggaaa gttgcgcgct gcgctggtcg aaccgggtgg ggagaaagag caggagtaag
     2761 aagaggatta agagtaggag caggatgggc tgctcggcag cgggccggca tcggccgcgg
     2821 ctcgccgctt aacgaaaagg cccgcggctg gtggtgaaac gaaaggagcc ctggccaata
     2881 tccttagaac ttgcactcga agccgaggct caggccctgg acccaatagg cttcgcggtt
     2941 gaaggtgaac tgcgggcggg tggggttgtt gcccaggtcg atcgtggggt cgatggcgtc
     3001 gccggggcgc atgacatcgt gccagtagat gaaggtgtag
//
LOCUS       DASUGB010000274         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_448261, whole genome shotgun sequence.
ACCESSION   DASUGB010000274 DASUGB010000000
VERSION     DASUGB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448261"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV87_05255"
     CDS             <1..342
                     /locus_tag="VFV87_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5103194.1"
                     /translation="LHAKRSIGVGGHISADDASAASAYEEGMRRELAEEVVIETPYRG
                     RLVGLINDDETEVGRVHLGVVHLFDVERPAIRPREDEIIEAGFLPVDDLLNDLPRFES
                     WSQICLKALFA"
     gene            431..1339
                     /locus_tag="VFV87_05260"
     CDS             431..1339
                     /locus_tag="VFV87_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5103195.1"
                     /translation="MQKSELSRRMMLGTMAAGSALAVTNLAHADPASDDPNWKITRGR
                     INQSVVHWCFKPLPVEDLARAAAGLGIKSVELVPPSDWPILKKYGLTCAIAGSHGFVK
                     GWNDKANWDFCTEKITQSIDAAAEFGCKSVITFSGMRGELPAAAEGDEIGKKNFVEGI
                     QRVLPLAEQKKVTLALEMLNSRVDIEMKGHPGYQCDSIEWAVDVCNRVGSDRLKILYD
                     IYHVQIMQGDVITRLKQYKDYIAHYHTAGNPGRNEIDDTQEINYPGIMRAIAETGYTG
                     FVGQEFIPTWKDPIAALRHAARVCDV"
     gene            complement(1355..>2526)
                     /locus_tag="VFV87_05265"
     CDS             complement(1355..>2526)
                     /locus_tag="VFV87_05265"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5103196.1"
                     /translation="VLPGDGGTLAYGWGLGVSMFLAAWLSPILGAMADARANKRTWLL
                     CTAFTGAACSVLLGVVPVGWPFGPAAFVALFFCASLAFELSFSFYNGFLPEIADETTM
                     NRYSGYGYAAGYFGGGLALAVAIAVLSIGSGYGLTTETGLRIGLVIMGLWWAVFTLPA
                     AFILRDRNPPRGKAAGFVETAQIAIGEVLSTLGHIRAYSVLALFLLGFLLFNEGIQTV
                     MSQASVFADEVLKMPPGELGLLVLMIQFVATPAAIGVGWLADRLGPRATLLGCLAIWC
                     GLLIVAFYTTTKLQFWCLGVVLALVMGGTQSVSRAIMGAMTPLAKTAEFFGFFNLSCR
                     ATSMVGPILFAQVLVWTKSANAAILSLLVFIVAGMLIVARVNLRRGIEQAKAAAS"
BASE COUNT          519 a          822 c          805 g          380 t
ORIGIN      
        1 ctgcatgcca agcggagcat cggcgtcggc gggcacattt cagccgatga cgccagcgcg
       61 gccagcgcct atgaggaagg aatgcgccgc gagctggccg aggaagtagt gatcgaaacg
      121 ccctaccgcg ggcggctcgt cgggctgatc aacgacgacg agaccgaagt cggccgcgtc
      181 catctgggcg tcgtccacct gttcgacgtc gagcgtcccg ccatccgccc ccgcgaagac
      241 gaaatcatcg aagccggctt cctgcccgtc gatgacctgc tcaatgatct gccgcggttc
      301 gagtcgtggt cgcagatctg tttgaaggcc ctgtttgcgt agggtgggct gtgcccaccg
      361 ctgtgaattg tcgcaatcga tctggtgggc acagcccacc caacgagtgt ttgcgtgagg
      421 agtggctgcc atgcaaaagt cggaactgtc gcgccggatg atgctgggaa ccatggccgc
      481 cggaagtgct ctggccgtga cgaatctcgc ccacgctgat cccgcgagcg acgatccgaa
      541 ctggaaaatc acccgcgggc ggattaacca gtcggtcgtc cattggtgct tcaagccgct
      601 gccggtcgag gatttggccc gtgcggcggc ggggctgggg atcaagtcgg tcgagctggt
      661 tccgccttcc gactggccga tcctcaagaa atacggcctg acctgcgcca tcgccggcag
      721 ccacggcttt gtgaagggct ggaacgacaa ggcgaactgg gacttctgca ccgagaagat
      781 cacgcagtcg atcgacgcgg cggccgagtt cggctgcaag tcggtcatca cgttctccgg
      841 catgcgcggc gagctgccgg cggcggcgga aggggacgag atcggcaaga agaacttcgt
      901 ggaagggatc cagcgcgtcc tgccgctcgc ggagcagaag aaggtgacgc tggccctgga
      961 gatgctcaac tcgcgcgtcg atatcgagat gaaggggcat ccgggctacc agtgcgattc
     1021 gatcgagtgg gccgtcgatg tctgcaatcg cgtcggctcc gaccggctga aaatcctgta
     1081 cgacatctac cacgtgcaga tcatgcaggg ggacgtgatc acgcggctga agcaatacaa
     1141 ggactacatc gcccactacc acacggccgg caatcccggc cgcaacgaga tcgacgacac
     1201 gcaggaaatc aactacccag gcatcatgcg ggccatcgct gagaccggct acaccggctt
     1261 cgtcgggcag gaattcatcc ccacctggaa agaccccatc gccgccctgc ggcacgcggc
     1321 acgcgtgtgc gacgtctagg aatagaggcc acgcttagct cgccgctgcc ttcgcctgct
     1381 cgatcccccg tcgcaagttg acgcgggcga cgatcagcat gccggcgacg ataaacacca
     1441 gcaggctcaa aatggccgcg ttggcgctct tggtccagac cagcacttgg gcgaacagaa
     1501 tcggcccgac catgctcgtc gcccggcacg agaggttgaa aaagccgaag aattccgccg
     1561 tcttggccag gggcgtcatc gcccccatga tggcccggct gaccgattgt gtgcccccca
     1621 tgaccagcgc cagcacgacg cccaggcacc agaactgcag cttggtcgtc gtatagaagg
     1681 ccacaatcaa caggccgcac cagatcgcca ggcagccgag cagtgtcgcc cgcggcccca
     1741 gtcgatcggc cagccagccc acgccgatgg cagccggcgt ggcgacgaac tggatcatga
     1801 gcacaagcag accgagctcg ccgggcggca ttttgagcac ttcgtcggcg aataccgacg
     1861 cctggctcat caccgtttga atgccctcat taaacagcag aaagcccagc aggaagaggg
     1921 ccagaacgct atacgcgcgg atgtggccga gcgtgctgag cacttcgccg atcgcgatct
     1981 gagcggtctc gacgaaaccc gcggcctttc cgcgaggcgg attgcggtcg cggaggatga
     2041 acgccgccgg cagcgtgaaa acggcccacc acagccccat gatcaccagg ccgattcgca
     2101 gccccgtctc ggtcgtcagc ccgtagccgc tgccgatcga gagcacggcg atggccaccg
     2161 ccagcgccag cccgccgcca aaatatccgg ccgcgtagcc gtagccggaa tagcgattca
     2221 tcgtggtttc atccgcgatc tccggcagaa agccgttgta gaagctgaag gaaagctcga
     2281 acgcgaggct ggcgcaaaag aagagggcta cgaacgcagc cgggccgaat ggccacccta
     2341 cgggaacgac gcccaagagg acgctgcacg cggctccggt gaacgcggtg cacaggagcc
     2401 acgtccgctt gttggcgcgg gcgtcggcca tcgcgcccag aatcggcgag agccacgcgg
     2461 ccaggaacat cgacacgccc agcccccagc catacgccag cgttccgccg tcgcccggca
     2521 ggacga
//
LOCUS       DASUGB010000275         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_30998,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000275 DASUGB010000000
VERSION     DASUGB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30998"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /gene="pheT"
                     /locus_tag="VFV87_05270"
     CDS             <1..1764
                     /gene="pheT"
                     /locus_tag="VFV87_05270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5103197.1"
                     /translation="TSIEVECPDLCPRYTARLIRGVKVAASPAQIAVRLVTVGQPVIN
                     NIVDITNYVLMECGQPLHAFDFHKLGGRKIVVRRARGGEQLEAIDHKTYTLDPEMCVI
                     ADDSHPVALGGVMGGAATEVSKDTVDVLIEAAQFAPMSIRATARQLKLHSPSSYRFER
                     GTDPEMVDWASRRCCELILKHAGGELVEGVIDIGSPREKRPPITLRLSQLKRILGIDV
                     PADAVRRILTALGCAEQSANAKEVITIPPSWRRDLEREIDLVEEVARVHGYDKIPEDA
                     SVPMAASHKPDSDRVLALVRGVLTAGGFDEALTASVVSERWSDSFSPWTDRPPLVTNS
                     PMLENSDRLRRSLVPSLLDVRRINESLSNPVVELFEIARVYLSTGGDLPREQPTLTAV
                     SGRGFLHVKGVVEALLSALHIDQPLELIDYQHELLTPGQACELRLGGEPRPMVAALGF
                     LGEVSPAGQKAFGLRGAATILEIDLSQLAAAAKLAPKYAEQSPFPTIARDINLIVAEE
                     VRWADLAATARSAAGPDLERLEYLDTYRDPAKDGPNTKRLLFSLTFRAKDRTLTGPEA
                     DTARDAVVAACTKRFAARLLS"
     gene            complement(1922..3310)
                     /locus_tag="VFV87_05275"
     CDS             complement(1922..3310)
                     /locus_tag="VFV87_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5103198.1"
                     /translation="MFGAIREWLTTRKLNRLIAALEDSFQCEDVAKELGALGDRRAVL
                     PLVQALEHERYGRNYRAIIAALAALQDPRAAEALVAGYNEVDHETKEVIRRVLSRTGE
                     EGYCTLVGALADHEEKVRISAASLLGELGGPIRARAVKALTPLLHDSSKYVRGAALGA
                     LQALGWQPATATERAAAAVAAGDFGAAADQGPASLPLLEREFMSHHQSEWPLKLTIAE
                     AVLKVDGNLAAKLVMQLLFRENLKWVDWLALADEAARFRDDRVFAAILAALQDPEMRR
                     KAALMLGKIRDPRAVEPLVAAVLALASYNSDAEVIAEALRELGDRRALNALMAQFRRG
                     GQVERPIVVKVIDMLGWEPEAEEDRTLLTAERLHLQVGAAIALLQIASNDSREREMEE
                     LLRTRAGEMTSEDLKALTLLPPMVTRTEIDHDDDHNHSGILRSYELRTDNSHVIALAA
                     AELHRRNRSATG"
     gene            complement(3501..>4535)
                     /locus_tag="VFV87_05280"
     CDS             complement(3501..>4535)
                     /locus_tag="VFV87_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5103199.1"
                     /translation="RLPFAQGESPPTIALPEGYSSAVLLAAQPDVPGAWLALASWCRA
                     RSIRRFDAHTQTLEESDLSPAGKFDGPPGFVSTEVMVKSHDGVLVPLTIVHRQDLELD
                     GSHPVLLSGYGAYGFSVPVNYSPLQLAWLERGGIFARAHVRGGGEFGKEWHLAGQKAT
                     KPNTWKDFIACAEYLVAHRYTSPGKLAGAGGSAGGILIGRSITEHPDLFGAALVEVGV
                     LDALRMETTTNGVPNIQEFGSVATRPGFDALLAMSALHHVRDGVKYPAVLLTHGINDH
                     RVEPWMSAKMTARLQAATASGKPILLRIDYAAGHGASLGATKEQALAQLADQWSFLLW
                     QFGDPAFQPK"
BASE COUNT          786 a         1497 c         1471 g          781 t
ORIGIN      
        1 acgagcatcg aggtcgagtg ccccgacctc tgcccgcggt acacggcccg gctgatccgc
       61 ggcgtcaagg tcgccgcctc acccgcccag attgccgtgc ggctggtgac ggtcggccag
      121 ccggtgatca acaacatcgt cgatatcacc aactacgtgc tgatggagtg cggccagccg
      181 ctgcacgcat tcgatttcca caagctcggc gggcgaaaga tcgtggtccg ccgcgcgcgg
      241 gggggcgagc agctcgaagc aatcgaccac aagacctaca cgctcgatcc cgagatgtgc
      301 gtcattgccg acgattcgca cccggtcgct ctgggcggcg tgatgggcgg ggccgcgacc
      361 gaagtctcca aagacacggt cgatgtgctg atcgaagcgg ctcagttcgc gcccatgtcg
      421 attcgcgcga ctgcccgcca gctcaagctg cacagcccct cgtcgtatcg cttcgagcgc
      481 ggcaccgatc ccgaaatggt cgattgggcc agccgacgct gctgcgagct gatcctcaag
      541 cacgccggcg gcgagctggt cgagggcgtc atcgacatcg gttcgccgcg cgagaagcgc
      601 ccaccgatta cgctgcgcct gtcgcagctc aagcggattc tgggcatcga cgtgccggcc
      661 gacgcggtcc ggcggatcct cacggctctg ggctgcgcgg agcaatcggc caatgccaag
      721 gaggtcatca cgatcccgcc gagctggcgg cgcgacctgg aacgcgagat cgacctggtc
      781 gaggaagtcg cccgcgttca cggctacgac aagattcccg aggacgccag cgtgccgatg
      841 gccgccagcc ataagccgga ctccgaccgc gtgctggcgc tggtccgcgg cgtgctgacc
      901 gccggcggct tcgacgaggc gctgacggcg agcgtcgttt ccgagcggtg gagcgattcg
      961 ttcagcccct ggaccgaccg gccgccgctg gtgacgaatt cgccgatgct ggaaaactcc
     1021 gaccggctgc ggcggagcct ggtgccgagc ctgctcgacg tgcggcggat caacgaatcg
     1081 ctcagcaacc cggtggtcga gctgttcgag atcgcccgcg tttatttatc gacgggcggc
     1141 gacctgccgc gtgagcagcc gacgctcacc gccgtcagcg ggcgcgggtt tttgcacgtc
     1201 aaaggcgttg tcgaagcact gctcagcgcg ctgcacatcg accagccgct ggagctgatc
     1261 gattatcagc acgagttgct gacgccgggg caggcgtgcg agctgcggtt gggcggggaa
     1321 ccgcgaccaa tggtcgcggc tttgggcttt ttgggcgaag tgtcgccggc ggggcagaag
     1381 gcgttcggcc tccgcggagc ggcgacgatc ctggaaatcg acctctcgca gcttgccgcg
     1441 gcggcgaagc tcgcgccgaa gtacgccgag cagagcccgt tcccgacgat cgcccgcgac
     1501 atcaacctga tcgtcgccga agaggtccgc tgggcggatc tggccgcgac cgcccgctcc
     1561 gcagccgggc cggacctgga gcggctggaa tatctcgaca cctaccgcga cccggccaaa
     1621 gacggcccaa acacgaaacg gctgctcttc tcgctcacct tccgcgcgaa agaccgcacg
     1681 ctcaccggcc ccgaagccga caccgcccgc gatgcggtcg tcgccgcctg caccaagcgc
     1741 ttcgccgccc ggctactctc gtagcttgaa gccaggcggc cgcctgatac gggcgacgaa
     1801 gttccgcgat ggccttatcg agcgtcgcgc ccttgcagcc ggacgaaaca gctcagaagc
     1861 cgatggttcg gctcgccggc ttattcccga cggtgtgcta cttccggatc ccgcaacacg
     1921 ctcagcccgt cgccgatcgg ttgcggcggt gcagttctgc ggccgcgagc gcgatgacgt
     1981 gcgagttgtc ggtcctcaac tcataggacc gaagaatacc gctgtggttg tggtcgtcgt
     2041 catggtcgat ctcggtccta gtcaccatgg gcggcaggag tgtgagcgcc ttgagatcct
     2101 cggaggtcat ctcgccggcg cgggtgcgca atagctcctc catttctctt tcccgagagt
     2161 cgttggaggc aatttgaagg agggcaattg ccgccccgac ctggaggtgg agtcgctcgg
     2221 cggtgagcaa cgttcggtcc tcttcggctt ccggctccca ccccagcata tcgatcacct
     2281 tgaccacgat cggacgttcc acttgaccgc cgcgccggaa ctgagccata agtgcattga
     2341 gtgcgcggcg gtcgcccagt tcacggagcg cctcggctat cacctcggca tcggagttgt
     2401 aggaggccag agcgagcacg gccgcgacga gcggttcgac cgcccgtggg tcgcgaattt
     2461 tccccagcat gagcgctgcc ttccggcgca tttccggatc ctggagagcg gcgagaatcg
     2521 cggcgaacac tcgatcatcg cgaaaccttg ctgcctcgtc ggccaaggcc agccaatcta
     2581 cccacttgag attttccctg aacaacagtt gcatcacgag tttcgctgcg aggttcccgt
     2641 ccaccttcag tacggcctcc gcgatggtca gcttgagcgg ccattccgac tgatgatggg
     2701 acatgaactc ccgttccaga agcgggagcg atgccggtcc ttggtccgcc gccgcgccaa
     2761 aatcgccggc ggcgacagcc gccgccgccc gctctgtggc cgtggcgggt tgccaaccca
     2821 aggcttgcaa cgcgccgagc gcggcgcccc gaacatactt cgaggagtcg tggaggagcg
     2881 gtgtcagagc cttgaccgcc cgcgccctga tcgggccacc caattcgccg agcagcgatg
     2941 cggcagaaat gcggactttc tcttcgtgat cggccagcgc gccgacaaga gtgcagtatc
     3001 cctcttcccc tgtgcgggac aatactcgcc gaataacctc tttggtttcg tggtcgacct
     3061 cgttgtaccc ggcgacaagc gcctccgccg cgcgtggatc ctggagggcg gcgagcgccg
     3121 cgatgatcgc cctataattc ctaccatacc gctcatgctc cagcgcctgt accagcggca
     3181 ataccgctcg ccggtcgccc aaagccccga gttccttggc aacgtcttcg cactggaagg
     3241 aatcctcaag cgcggcaatc agccggttca actttcgggt cgtcagccac tcacggatcg
     3301 caccgaacat ttagctgcct ttcgatcaac acttcccgtg caacacttcc cctgtacgac
     3361 taaactggtc gaaccgtagg tcggtgacga taagcattag tgttccgagc gtgaacgacc
     3421 gcaggaggcc gaaccagtgt ttatcgcgca tgtttgatcg atattgtgga aacggcccgg
     3481 aaaggaagtc aaggaaaggc tcacttcggc tggaaggccg gatcgccgaa ttgccagagg
     3541 agaaagctcc actggtcggc gagttgggcg agggcctgct ctttggtcgc gcccaggctc
     3601 gcgccgtggc cggcggcgta gtcgattcgc agcaggatcg gtttgccgct ggcggtggcc
     3661 gcttgcaggc gggcggtcat cttggccgac atccagggct cgacgcggtg gtcgttgatg
     3721 ccgtgcgtga gcagcacggc ggggtacttc acgccgtccc gaacgtgatg cagggcgctc
     3781 attgcgagca aggcgtcgaa tcccggcctg gtcgccacgc tgccgaattc ctgaatgttg
     3841 gggacgccgt tggtcgtcgt ctccatgcgg agcgcgtcga ggacgccgac ttcgaccaaa
     3901 gccgctccaa acaggtcggg atgctcggtg atcgagcggc cgatcagaat tccccccgcg
     3961 ctgccgccgg cgccggcgag cttgccgggc gaggtgtacc tgtgggctac gaggtattcg
     4021 gcgcaggcga tgaagtcctt ccaggtgttg ggcttcgttg ctttctgccc cgctaagtgc
     4081 cactctttgc cgaactcgcc gccgccgcgc acgtgggccc gggcaaagat accgccccgc
     4141 tcgagccagg caagctgtag cggcgaatag ttgacgggga ccgaaaagcc atacgcccca
     4201 tagccggaaa gcagcaccgg gtgcgaaccg tccagttcca gatcctgccg atgcacgatc
     4261 gtcagtggga ccagcacgcc gtcgtggctc ttgaccatca cctcggtcga gacaaagccc
     4321 ggcggtccat cgaacttgcc ggcaggcgac aggtcggatt cttccagggt ctgggtatgc
     4381 gcgtcgaacc ggcgaatcga cctggcccgg caccacgagg ccagcgccag ccacgcgccc
     4441 ggcacgtcgg gctgggcggc cagaagtacg gcggaggaat agccttccgg cagggcgatc
     4501 gtgggcggcg attcgccctg ggcaaacggc aggcg
//
LOCUS       DASUGB010000276         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1281482, whole genome shotgun sequence.
ACCESSION   DASUGB010000276 DASUGB010000000
VERSION     DASUGB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281482"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="trpA"
                     /locus_tag="VFV87_05285"
     CDS             complement(<1..652)
                     /gene="trpA"
                     /locus_tag="VFV87_05285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5103200.1"
                     /translation="MSRLDQLFTDLRRQERKAFMPFVTAGDPDLDFTADVIRELDARG
                     SSLCEVGIPYSDPIADGPVIQASYTRALANKIKLADIFAMLAKVSPQVKMPLVTMVSY
                     AIIYRHGLQKYVEDAQAAGIAGAIVPDLLVEESAELAAICKAADFALIQLVTPTTPRE
                     RAVRIAQASTGFLYYVSVTGITGERTELPPSLVDNVAWLRTKTDLPICIGFGISQPK"
     gene            complement(655..1068)
                     /locus_tag="VFV87_05290"
     CDS             complement(655..1068)
                     /locus_tag="VFV87_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX5103201.1"
                     /translation="MCSDGDTRPILRAEILLPDSAWLEVRFLIDTGADRTVFSADVLG
                     ASRLQSVSTRGHIGGIGGVVETEYIRTQIRFRRDDGVQVVFHGEYAALTDPDALEMSV
                     LGRDILNLFAVIVDRPGDVVAILGGHHYYSIQRRP"
     gene            complement(1082..1390)
                     /locus_tag="VFV87_05295"
     CDS             complement(1082..1390)
                     /locus_tag="VFV87_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103202.1"
                     /translation="MIQHALKPPSLEMFEVTDPVELAAAAIRRTHFDRNSAWLQQHIK
                     EVGADNHRGKFYCIAGQQAFVGDTVEDVVARAKAAHPDDQGWFTGYVPRERVPRIYAC
                     "
     gene            complement(1423..2646)
                     /gene="trpB"
                     /locus_tag="VFV87_05300"
     CDS             complement(1423..2646)
                     /gene="trpB"
                     /locus_tag="VFV87_05300"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5103203.1"
                     /translation="MSTATGTPSLQPAQVPDTAGRFGAFGGRYVPETLTRALDELTVE
                     YEKARQDPQFQADLDDLFQHYVGRPSPLYFARRLTEQCGGAKIWLKREDLNHTGAHKI
                     NNTLGQALLTLRMGKKRVIAETGAGQHGVATATACAHFGLDCVVYMGEEDIRRQAPNV
                     NSMKLLGAEVRPVSSGSKTLRDAVNDALRDWMASVETTHYIIGSVIGPHPFPRIVRDF
                     QSVIGREARQQSLQRIGRLPDLVVACVGGGSNAAGMFYPFIGDQSVRLLGVEAGGRGP
                     RPGDHASPLSYGSPGVLHGSYSYVMQDEDGQTCDVHSISAGLDYPGVGPEHSYWKDIG
                     RVTYTFCEDKDALTGFDALARNEGILPALESSHAIFRAMEQARQMPPDQHIVVCLSGR
                     GDKDAAEIARLRGTR"
     gene            2796..>3037
                     /locus_tag="VFV87_05305"
     CDS             2796..>3037
                     /locus_tag="VFV87_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5103204.1"
                     /translation="MPELPEVETMRRGIAAIAGSRVAGAEKLRCKRRPIAIEPRGLGR
                     RLIGRRIEEVGRLGKRVVLRLEDGGRLIFEPRMTGLV"
BASE COUNT          561 a          942 c          982 g          552 t
ORIGIN      
        1 gcttcggctg gctgatgccg aagccgatgc agatcggcag atcggtcttc gttcgcagcc
       61 aggcgacgtt gtcaaccagc gacggcggca gctcggttcg ttcgcccgta atgccggtga
      121 ccgagacgta atagaggaat cccgtgctgg cctgggcgat ccgcacggct cgctcgcggg
      181 gagtggtggg cgtgacaagc tggatcagag cgaagtccgc tgccttgcag atcgccgcca
      241 gctcggccga ttcctcgacc agcagatctg gcacaatggc cccggcaatg cccgccgcct
      301 gggcatcctc gacgtacttc tgcaaaccgt ggcgatagat gatcgcgtag ctcaccatcg
      361 tgaccagcgg catcttcact tgcggcgaca ctttggccag catcgcgaag atatcggcca
      421 acttgatctt gttggcaagc gcccgcgtgt acgaggcctg aatcaccggc ccgtcggcga
      481 tcggatcgct ataggggatg ccgacttcgc acagcgaaga gcctcgagcg tccaactcgc
      541 ggatcacgtc cgccgtgaag tccaaatccg gatcgccagc ggttacgaac ggcatgaagg
      601 ccttgcgctc ctggcggcgg aggtcggtga agagttggtc gaggcgggac atcgctatgg
      661 gcggcgctgt atggagtaat aatggtgacc gcctagaata gcgacgacat ctcccggtcg
      721 atcaactatc accgcaaaca gattaaggat gtcgcgaccc agaacgctca tctccaaagc
      781 atcgggatct gtaagagccg cgtattcgcc gtggaaaacg acctgcacgc catcgtctcg
      841 ccgaaatcga atctgagttc tgatatattc tgtttccacc acgcccccaa tgccaccaat
      901 gtggccgcgg gtggaaaccg actgcagtcg cgacgcgccg agcacgtcgg cactgaagac
      961 cgtgcgatcg gcgccggtat cgatcagaaa tctcacctcc aaccaggcgg aatcgggcag
     1021 gagaatctcg gcgcgaagaa tcgggcgagt gtcgccgtcc gagcacactc cccattctcc
     1081 gtcaacacgc ataaatcctc ggcactcttt cgcggggaac ataaccggtg aaccaccctt
     1141 ggtcatcagg gtgagcagcc ttggcccgcg ctacgacgtc ttcgacagta tcgccaacga
     1201 atgcctgttg tcccgcgatg caatagaact tgccgcggtg gttgtcggcg ccaacctcct
     1261 tgatgtgctg ctggagccac gcgctgttcc gatcgaaatg cgtgcggcgg atggcagccg
     1321 ctgccaactc aaccgggtca gtgacctcga acatttcgag ggacggaggc ttgagagcgt
     1381 gttggatcat cccgttatcc tagcatcgtt ttccaatccg cgttacctcg ttccacgcag
     1441 ccgggcaatc tccgccgcgt ctttgtcccc gcggccggag aggcagacga caatgtgctg
     1501 gtccgggggc atctgccggg cctgctccat ggcgcggaag atcgcgtggg agctttccag
     1561 ggccggcagg atgccctcgt ttctggcgag ggcgtcgaag ccggtgaggg cgtctttgtc
     1621 ttcacagaac gtgtaggtca cacggccgat gtccttccag tagctgtgct cggggccgac
     1681 gccggggtaa tcgagccccg ccgagatcga atgaacgtcg cacgtctggc catcttcgtc
     1741 ctgcatcacg tagctgtagc tgccgtgcag cactccgggc gagccatacg acagcggcga
     1801 ggcgtggtcg ccgggccgcg gcccgcgccc acccgcttcg acgccgagca gccgcacgct
     1861 ctggtcgccg atgaagggat agaacatgcc ggcggcgttg ctgccgccgc cgacgcaggc
     1921 cacaacgagg tccggcagcc ggcctatgcg ctggagcgac tgctggcggg cctcgcggcc
     1981 aatgaccgac tgaaaatcgc ggacaattcg cggaaacggg tgcggcccga tcaccgagcc
     2041 gatgatgtag tgcgtcgttt cgacgctggc catccagtcg cggagggcgt cgttcacggc
     2101 gtcgcgaagc gttttgctgc cgctgctcac ggggcggact tccgcgccga ggagcttcat
     2161 gctgttgacg ttcggggcct ggcggcggat atcctcctcg cccatataga cgacacaatc
     2221 gaggccaaaa tgggcacaag ccgtcgcggt cgccacgccg tgctgccccg cgccggtctc
     2281 ggcaatcacc cgcttcttgc ccatccgcag cgtgagcagc gcctggccca gcgtgttgtt
     2341 gatcttgtgg gcgccggtgt gattcaggtc ctcgcgcttc agccagatct tcgccccgcc
     2401 gcactgctcg gtcagtcggc gcgcgaaata gagcggcgac ggccggccga cgtagtgctg
     2461 aaacaggtcg tccaaatcag cctgaaactg gggatcctgc cgggcctttt cgtactcgac
     2521 ggtcagctcg tccaaggccc gcgtcagcgt ttctggcacg taccggccgc caaaggcgcc
     2581 gaaacgcccc gcggtgtcgg ggacctgggc tggctggagg ctgggcgtgc cggtcgcagt
     2641 ggacattacg aaggatcagg aaagggagtg gcgagactcg attctcaatg cggctcgtcg
     2701 gatggtcaag ggcggcgtgc attggacatg cccactgtac gatgtcccac acaatcagta
     2761 ctcagtagga gttgaacgag ttccgcggag gttccatgcc tgagctgccc gaagtcgaga
     2821 cgatgcgccg aggaattgcc gcgatcgccg gcagccgggt tgcgggggcc gaaaaattac
     2881 gctgcaaacg ccggccaatt gcgattgaac cacgcggcct gggtcgccga ctgatcggcc
     2941 ggcgaattga ggaggttggc cggctgggaa agcgcgtcgt cctgcggctg gaggacggcg
     3001 ggcgattgat cttcgagccg cggatgacag ggctggt
//
LOCUS       DASUGB010000277         4734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_170166, whole genome shotgun sequence.
ACCESSION   DASUGB010000277 DASUGB010000000
VERSION     DASUGB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170166"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFV87_05310"
     CDS             <1..609
                     /locus_tag="VFV87_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103205.1"
                     /translation="YTDPNSGVTGDYTTHYYGVMGPGGASDNFQIVVGGITYQYRYGS
                     SGGNGAWSYHGILSHYRETTGSISTFRVVRMADVLDGTANTLMLGEISKNLPILNPST
                     GATQANQYRTWLRGNTHASGSSATGSGATKNIRYPINSTFYNGSNNFNEISFGSDHAG
                     QGAMFANADASVRFVRQNIDLTLYIFASSMNGNEAAPLGSSQ"
     gene            970..1995
                     /locus_tag="VFV87_05315"
     CDS             970..1995
                     /locus_tag="VFV87_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103206.1"
                     /translation="MCRRLHLRTHTGFTLVELLVVIAIIGILVALLLPAIQAAREAGR
                     RSSCSNNIRELAIGLHNFHDVHDKFPRGAENAVYPDPNPTGSTTAFNGTSWIVHILPF
                     IEQKGLYDRYRFDLAYNSSNNAAIGHEVLKVLYCPSGPDPQKYLDPNGAPINGSVTTH
                     YYGVMGPSGPSDNFQIVVGGITYQYRVGNSTDNATWSYHGILSQYRDTTGSISTKRVV
                     RMADVLDGTAHTLMLGEISKTLPIFNNATGMTQANQYRAWTRGNSGGSGATKNVRYPI
                     NSTFYNGSNNFNNLSFGSDHAGDGAMFAMADASIKFIRENIDLTTYTFAASMNGNEPA
                     LLDANQR"
     gene            2289..3314
                     /locus_tag="VFV87_05320"
     CDS             2289..3314
                     /locus_tag="VFV87_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103207.1"
                     /translation="MGRRLVCRAHRGFTLVELLVVIAIIGILVALLLPAIQAAREAGR
                     RSSCSNNLHELAIGLHNFHDVFDRFPAGAQQDTLPDPNPTGSTSYFRGTSWIVHILPF
                     IEQKGLYDRYRFDLAYNSSPNAAIGHEVLKVLYCPSGPDPQKYRDPNGGAIRDAVTTH
                     YYGVMGPAGPSDNFRITVGGIVYQYREGDATNNAAWSYHGILSQYKDRPGSISTKRVV
                     RMADVLDGTAHTLMVGEISKTLPIFNNATGATQPNQYRAWVRGNSGGSGATKNVRYPI
                     NSTFYNGSNNFNNLSFGSDHAGDGAMFAMSDASVRFIRENIDLTTYIFASSMNGNEPA
                     LVDANQR"
     gene            3595..4407
                     /locus_tag="VFV87_05325"
     CDS             3595..4407
                     /locus_tag="VFV87_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103208.1"
                     /translation="MTRESMTRETMTCPSPTLGQKLGRVRSIPELIQAVICLAAVGGF
                     IVLWRQDAAPVLVLAVGAIGAAVALCGAAYCLPVICSSLTIYEGGLELNVGGKTTVIP
                     FEALQRLETEFTDQRWKGQYVGTRARLQFFVDGRTRPHNYTCDYRHGQRKERTAMLAI
                     DKCHEAIQMRLLAELEQKGALPWRGNVSLTAEGIKLTDSPISSRLVPFREISAWKLVE
                     DNFKIWKGDDALPCVVVPSDTPNFFPLCGLFELLCSAQIEPAADAEPELAQA"
     gene            complement(4492..>4734)
                     /locus_tag="VFV87_05330"
     CDS             complement(4492..>4734)
                     /locus_tag="VFV87_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103209.1"
                     /translation="WRGRFFGVWQGVPFDHTVTFSGPPDNLRGTATIDNAHYDWTGQI
                     DVFQKPARFSGAFGGTRYAGQFDLQSKPGRASASLR"
BASE COUNT          920 a         1555 c         1409 g          850 t
ORIGIN      
        1 tacacggacc ccaattccgg cgtgaccggt gactacacga cccactatta cggcgtgatg
       61 gggccgggcg gcgccagcga taatttccag atcgtcgtcg gcggcattac ctatcaatac
      121 cgctacggct cgagcggcgg caacggcgct tggtcgtacc acggcatcct cagccactat
      181 cgcgagacga ctggcagcat cagcacgttc cgcgtggtgc ggatggccga cgtcctcgac
      241 ggcaccgcca ataccttgat gctcggcgaa atctcgaaga atttgcccat tctcaatccc
      301 tccacgggcg caacgcaggc gaatcagtac cgcacgtggc tccgcgggaa cacgcacgcc
      361 agcggcagct cggcgaccgg cagcggtgcg accaagaaca ttcgttaccc aatcaactcc
      421 acgttctaca acggcagcaa caacttcaac gagatcagct ttggcagcga tcacgccggc
      481 caaggcgcga tgtttgcgaa cgccgatgct tcggtccggt ttgtccgcca aaacatcgac
      541 ctgacgctgt atatctttgc gtccagcatg aacgggaatg aggccgcgcc gctcggctcc
      601 agccagtaac gacgtcgcga gcagtggcaa ggtgagcagt gacaaggtga gcagtgacaa
      661 ggcgagcagt gacaaggcga gcagtgacaa ggtgagcagc gagtggtggc gacgggatac
      721 ggctcaccac tccctccgtt ttttcttccg gctgggccgc cgcctgtaat ttctggcaat
      781 tttcggccag cgtcgattga caccggggcg gcgcggaagt aaacctagcg acatggggtg
      841 atcgctgggg ttggcagctt ggggattctg tcttcttgcg ctgtgcaggg tcgcggcagc
      901 ctttcccggc attgctggtc cgttttgcat tgccgttcaa accttgtcct ggacatgaaa
      961 gggccgagta tgtgccgtcg tttgcacttg cgcacgcaca ccgggtttac gctggtggag
     1021 ctgctggtcg tgatcgccat cattggcatc ttggtggcct tgctgttgcc tgcgattcag
     1081 gccgcccgcg aagcgggccg ccgctcgagc tgctccaata acatccggga gctggccatc
     1141 ggtctgcaca atttccacga cgtccacgat aagtttcccc ggggcgcgga aaacgccgtt
     1201 tatcccgatc cgaacccgac cggcagcacc acggccttta atggcacgag ctggatcgtc
     1261 catatcctcc cgttcatcga gcagaaaggg ctgtacgacc ggtaccgctt cgacctggcg
     1321 tacaacagct cgaacaacgc cgccatcggg cacgaagtcc tcaaagtcct ctattgcccc
     1381 tcggggccgg acccgcagaa atacctggat cccaatggcg cccccatcaa cggctcggtc
     1441 acgacccact attacggcgt gatggggccg tcgggtccga gcgacaactt ccagatcgtc
     1501 gtgggtggaa tcacctatca gtaccgcgta ggcaattcaa ccgacaatgc cacgtggtcg
     1561 tatcacggca tcctcagcca gtaccgcgac accacgggca gcatcagcac caaacgcgtc
     1621 gtgcggatgg cggacgtgct ggacggaacc gcccacacgc tgatgctggg cgaaatctcc
     1681 aagaccctgc cgatcttcaa caatgcgacc ggcatgaccc aggccaacca gtaccgcgcc
     1741 tggacccgcg gcaacagcgg cggcagcggc gcgacgaaga acgtccgcta cccgatcaac
     1801 tccaccttct acaacgggag caacaacttc aacaatctca gcttcggcag cgaccacgcc
     1861 ggcgacggcg ccatgtttgc catggccgac gcctcgatca agttcattcg cgaaaacatc
     1921 gacctgacca cctatacctt cgcggccagc atgaacggca atgagcccgc gctgctcgac
     1981 gcgaatcaga ggtaaaaggt taaaggtgcg tggcgagtaa gtcagtggtg cgtgatgagt
     2041 ggcttgacca ctcaccacgc actcttcttt tggttttggc cttttggccc caagcgccaa
     2101 gaaaacggtt gactcacgac agccgccggg gtaaaggtaa cggttggtta gtcggatgcc
     2161 gttaaggcag cttggggatt ctttagccgg actgcgcgct cgcgcgctct ctagcggcgg
     2221 tctttcccgg catcctcgta ttgtcgcttg ggattcttca accttcctca tctgaaaggg
     2281 ggctgggtat gggccgccgc ttggtctgcc gcgcgcaccg gggatttacc ctggtcgaac
     2341 tgttggtcgt catcgccatc attggcattc tcgtggcttt gctgctgcct gcaattcagg
     2401 ccgcccgcga agccggccgc cgctcgagct gctcgaacaa cctccacgag ctggcgatcg
     2461 gtctgcacaa cttccacgac gtgttcgatc gatttccggc gggcgcccag caggacacgt
     2521 tgcccgatcc caaccccacc ggcagcacgt cgtacttccg cgggacgagc tggatcgtcc
     2581 acatccttcc gtttatcgag cagaaggggc tgtacgaccg gtaccggttc gacctggcgt
     2641 acaacagctc gccgaacgcc gccatcgggc acgaagtcct caaagtgctc tattgcccct
     2701 cggggcccga tccgcagaaa taccgcgatc ccaatggcgg cgctatccgg gacgctgtca
     2761 ccacccatta ctatggcgta atgggaccgg caggccccag cgacaacttc cgaatcacgg
     2821 tgggcggcat cgtttatcag tatcgcgaag gcgacgccac caacaatgct gcgtggtcct
     2881 accacggcat cttgagccaa tacaaggacc gccccggcag catcagcacc aaacgcgtcg
     2941 tgcggatggc cgacgtgctc gacgggaccg cccatacgct gatggtcggc gaaatctcca
     3001 agacgttgcc gatcttcaac aatgcgaccg gcgcgaccca gcccaaccag taccgcgcct
     3061 gggtccgcgg caacagcggc ggcagcggcg cgacgaagaa cgtccgctac ccgatcaact
     3121 ccaccttcta caacgggagc aacaacttca acaatctcag cttcggcagc gaccacgccg
     3181 gcgacggcgc catgtttgcc atgtcggacg cttccgtgcg gttcatccgc gaaaacatcg
     3241 acttgaccac ctacatcttc gcgtccagca tgaacgggaa cgagcccgcc ctggtcgacg
     3301 ccaaccagcg ctaacgggag acgctccaaa aggggacatt ctacttttcg gcgctcgagc
     3361 gctgaagcgg ggaatgtccc tttctcttgc gccgcgcgac cggcaggatc gcgatcgagc
     3421 caccgttcgg ctggaatctt cggcatattc gctacggcca ggacaagccc agccgtgccc
     3481 agaggccacg ccggcagcgc ggctccttcg gtgacgtaac cttctggggc gaagacctgc
     3541 cgcgccgcca gccgattcca cccgaccacc cgatcactcg tcccgagtca aatcatgacc
     3601 cgcgaatcga tgacccgcga aacgatgacg tgcccatcgc ccaccctggg gcagaagctg
     3661 gggcgagtgc gatcgatccc cgagctcatc caagccgtca tttgccttgc ggccgtggga
     3721 gggttcatcg ttctctggcg gcaggatgcg gcgccggtgc tcgtgctggc ggtcggcgcg
     3781 atcggggcgg ctgtcgccct gtgcggcgcg gcgtattgcc tgcccgtgat ttgctcctcg
     3841 ctgacgatct acgaaggcgg cctggaattg aacgtgggcg gcaaaacgac ggtcatcccc
     3901 ttcgaggcgc tccagcgcct ggagaccgaa ttcacggacc agcggtggaa ggggcagtac
     3961 gtgggaacgc gggccagatt gcagtttttc gtggacggcc gcacccggcc gcacaactac
     4021 acctgcgact atcgccacgg ccagcgcaag gagcggaccg cgatgctggc catcgacaag
     4081 tgccacgagg cgatccaaat gcgattgctg gccgagctcg agcagaaggg cgccctccct
     4141 tggcgcggca acgtttcctt gaccgccgag ggaatcaagc tcaccgattc gccgatctcc
     4201 tcgcggctgg ttcctttccg cgagatcagc gcctggaagc tcgtcgagga caacttcaag
     4261 atctggaagg gcgacgacgc gctgccctgc gtcgtggtgc cgagcgacac gccgaacttc
     4321 ttcccgctgt gcggcctgtt cgagctgctg tgcagcgccc agatcgagcc cgccgccgat
     4381 gccgagccgg aactggccca ggcgtagggt gggctccgcc caccacggcg cagcttcatc
     4441 gctgtgcaac tttatcgcgt gggtccggtg ggcacagccc accctacacg ctcagcgcag
     4501 ggaggcgctg gcacggcccg gcttggactg caggtcaaat tggccggcat agcgcgtgcc
     4561 gccgaaggcg ccgctgaagc gcgccggctt ctggaacacg tcgatttgac ccgtccagtc
     4621 gtagtgggcg ttgtcgatcg tcgcggtgcc gcggagattg tccggcgggc cgctgaacgt
     4681 caccgtgtgg tcgaacggca ctccctgcca gacgccaaaa aatcgccccc gcca
//
LOCUS       DASUGB010000278         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1837865, whole genome shotgun sequence.
ACCESSION   DASUGB010000278 DASUGB010000000
VERSION     DASUGB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837865"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFV87_05335"
     CDS             <1..472
                     /locus_tag="VFV87_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103210.1"
                     /translation="IAVTVNGGAPQFFGRAAVDVLIIDTDSGQDAITITGTDEDEVAH
                     FIPGQASLRVGTSFAGANYGILALDVETAEVTAGTGGTDLAVMRDTRTISDALVASAN
                     TATLTAGAGRLVRSIDFDQVRALTTIVTGGAEDDTADRNAINYVLELAGSWREI"
     gene            complement(559..1086)
                     /locus_tag="VFV87_05340"
     CDS             complement(559..1086)
                     /locus_tag="VFV87_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103211.1"
                     /translation="MPYRDDLNRGPLPDPLAYFITWSTYGTWLPGDQRGWVEYRRGWQ
                     LPDPILELEAAARMTEDACWLDPEQRQCAEAQVLETCTVRGWELWAVNCRTNHLHAVL
                     TADREPEVVRAQLKAWCTRRLKELDEVRRLEIPALPLRENWWAERGSQRYVNDHDGLE
                     AATLYVRDGQDRPRT"
     gene            complement(1145..2545)
                     /locus_tag="VFV87_05345"
     CDS             complement(1145..2545)
                     /locus_tag="VFV87_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103212.1"
                     /translation="MLTLHTGSTRDCSGLLRRDFVRAGALAIGGLALPQLLAAKEAAR
                     SSGKSYVRDRSIVLLFLGGGASHIETFNPNLDAPAPYCSITGEVKTSIPGLTLGGTFP
                     QLAVHADRLAVVRSFSHDNGNHPKAIIHVLSGGTDPTGDGKTGQSMGSAYARIRGANH
                     EASGLPTYCLLTSDEIDGQYRTERGRIEAGSRPGQLGLAYAPFDPSGGSQLLKNLSLN
                     LPVNRLDDRKSLLSKLDNAKREIDASGNVAGADRFEQQALDMIVRGAGEAFDTKREDK
                     RLIERYDTSSFRVGKKVFQPSALGRQMLLARRLVEAGSGFVTVQNSGWDMHADGNNPG
                     VAAGMEMLGRPLDKAVSAFLEDLDARGLADKVLLVITGDFGRTPTINQRGGRDHYPKL
                     CTLAFAGGGLARGQIIGQAGRKNDAPASDPIRPENLASTVLHALFDVGQLRLDPGVPR
                     DLARLAEAPPIAELAH"
     gene            complement(2595..2912)
                     /locus_tag="VFV87_05350"
     CDS             complement(2595..2912)
                     /locus_tag="VFV87_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103213.1"
                     /translation="MSNAPPLVPNTLGKASLVLGILASTLVVSVGLCAGVGQQQGWLK
                     QAGPLLFIVGGTFAFLGIVSALLGIGGLFGRHRSRATSIFGLILGLIAVAIFLAVVSA
                     ANR"
     gene            3131..4282
                     /locus_tag="VFV87_05355"
     CDS             3131..4282
                     /locus_tag="VFV87_05355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5103214.1"
                     /translation="MKQLSAKRPGGRPEPLRLALVITELEPGGAERCLVNLAVRLDRQ
                     RFAPVVYSLGRRPEPPKDLLVGQLEQAGVPVQFLDLRHWSQYAAGARRLAALMAQQRA
                     EVVQTFLFHANVLGCRAARRAGVQHVFTGVRVADPRRWRTALERLATGAAEKIVCVSQ
                     GVADFCRSRGFAAQKLVVIPNGVDVDRFQRAAAIDLTQFGVRAGRRAILFVGRLDRQK
                     GLDELLGMLPRVFEQVADCDLLLVGDGPERADLQRAAQVSGIQGRVQFAGWQADVPGI
                     LAASELLVLPSRWEGMPNVILEAMAAGKPVVAANVEGVAELLGALTAEQTAHAGDKSA
                     LASRLAVLLANPPRAKELGSLNQHRAMAEFSLDRMVQKFSDLFQGRFEA"
     gene            4467..>4694
                     /locus_tag="VFV87_05360"
     CDS             4467..>4694
                     /locus_tag="VFV87_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103215.1"
                     /translation="MMLTGTYERALDAKLRLAMPKRFREALAGQSPLVLTPGTDGSLA
                     LFAPAAFAALTQRLASRSPTGQDVRAFSRLLY"
BASE COUNT          779 a         1431 c         1639 g          845 t
ORIGIN      
        1 gatcgccgtg acggtcaacg gcggcgcgcc gcagttcttc ggccgcgcgg cggtggatgt
       61 gctcatcatc gacaccgatt cagggcagga tgcaatcacg atcaccggca cggatgaaga
      121 cgaggtcgcc cacttcattc cgggccaggc ttcgctgcga gtgggcacga gcttcgcggg
      181 agcgaactac ggcattctcg ctctcgatgt cgaaaccgcg gaagtgaccg ccggcaccgg
      241 cggcaccgat ctggcggtga tgcgcgacac ccgtacgatc agcgacgcgc tggtcgccag
      301 cgccaacacc gcgacgctga cggccggcgc cgggcggctg gtgcggtcga tcgacttcga
      361 ccaggtgcgg gccctgacga cgatcgttac cggcggcgcc gaggacgaca cagccgaccg
      421 caacgcgatc aattacgtcc tggagctggc cggaagctgg cgggagatct agcccatact
      481 aacccgaagc gtcagcgagg gagtccagcg aatggaggtt gatccctcgc ttacgcttcg
      541 cgcttggtgt ggcgctccct aggttcgcgg acgatcttgt ccatcccgga catatagagt
      601 cgctgcttcg agaccgtcgt ggtcgttgac ataacgctgg cttccgcgtt cggcccacca
      661 gttctcacgg agaggtaatg ccggaatctc caggcggcgc acttcgtcga gttccttcag
      721 acggcgggta caccacgcct tcaattgagc gcgaacgact tctggctcgc gatccgcagt
      781 cagtacggcg tgcaagtggt tagtccggca gttcacggcc catagttccc atccgcgtac
      841 ggtacacgtc tcaagaacct gtgcttccgc gcactggcgc tgctccggat caagccagca
      901 agcgtcctcg gtcatgcgcg cggccgcttc caactccaga attggatcgg gaagttgcca
      961 gccgcgccgg tactcgaccc agccccgctg gtcgccgggc agccacgttc cgtacgtgct
     1021 ccaggtgatg aagtaggcaa gcgggtcagg gagggggccg cggttgagat catcgcgata
     1081 gggcatgatt ccctcgctga cgcttcgggt tggtgtagcc ggacttcctc gcttacgctt
     1141 cgggttagtg tgccagctcc gcgattggcg gggcttcggc gaggcgggcg aggtcgcggg
     1201 ggacgccggg atcgaggcga agctggccca cgtcgaagag ggcgtggagc acggtggagg
     1261 cgagattctc gggccggatc gggtcgctgg cgggggcgtc gtttttgcgg cccgcctggc
     1321 cgatgatctg gccgcgggcc aggccgccgc cggcgaaggc gagcgtgcag agcttcggat
     1381 agtggtcgcg gccgccgcgc tggttgatcg tcggcgtgcg gccgaaatcg ccggtgatga
     1441 cgagcagaac cttgtccgcc aggccgcggg catcgaggtc ttcgaggaag gcgctgacgg
     1501 ctttgtcgag cgggcggccg agcatctcca tgccggcagc gacgcccggg ttgttgccgt
     1561 cggcgtgcat gtcccagccg gagttctgca ccgtgacgaa tccgctgccg gcttcgacca
     1621 ggcggcgggc caggagcatc tgccggccga gggcggaggg ctgaaagacc ttcttgccga
     1681 cgcggaacga gctggtgtcg taccgctcga tcagtcgttt gtcttcgcgc ttggtgtcga
     1741 acgcttctcc cgcgccgcgg acgatcatgt ccagggcctg ctgctcgaag cggtcggccc
     1801 cggcgacgtt gccgctggcg tctatctcgc gcttggcatt atcgagtttc gataacagcg
     1861 acttgcggtc atccaggcga ttgaccggca ggttgagcga caggttcttc aggagctggc
     1921 tgccgccgct gggatcgaac ggggcgtagg ccaggccgag ctgcccgggc cgcgacccgg
     1981 cttcgatgcg gccgcgctcg gtgcggtatt ggccgtcgat ttcgtcgctg gtgagcaggc
     2041 aataggtcgg caggccgctc gcctcgtggt tcgcgccgcg aatgcgggca taagccgagc
     2101 ccatgctctg gccggttttg ccgtcgccgg tcggatcggt cccgccgctc aaaacgtgga
     2161 tgatcgcctt ggggtggttg ccgttatcgt gtgaaaacga gcggacgacg gccagcctat
     2221 cggcgtggac ggctagctgc gggaacgtgc cgccgagcgt caggcccgga atgctcgttt
     2281 tcacttcgcc ggtgatcgag caatacggcg cgggcgcgtc cagattcggg ttgaacgtct
     2341 cgatatggct cgccccgccg ccgaggaaca atagcacgat cgagcgatcg cgcacgtagc
     2401 tcttgccgct ggagcgtgcg gcctctttcg ccgccaatag ctgggggaga gcaaggccgc
     2461 cgatggccag cgccccggcg cggacgaagt cccgccgcaa aaggccgctg cagtcgcgcg
     2521 ttgacccagt atggagcgtg agcatacgac tgtgatcgta tcagaagcgg ctgatgggtc
     2581 aatgttttta cggctcaacg attggcggca ctgaccacgg ccagaaagat cgcaacagcg
     2641 atcagcccca aaatcaagcc gaatatcgag gtcgcccgcg agcgatggcg gccaaacagc
     2701 ccgccgattc ccagcaaggc actcacgatg cccaggaagg cgaacgtgcc tcccacaatg
     2761 aacagcagcg ggccggcctg cttgagccag ccttgctgct ggccaacgcc ggcgcacagc
     2821 cccacgctga cgaccagcgt gctcgccagt atgcccagca cgaggctggc cttgcccagc
     2881 gtattaggca cgagcggcgg cgcgttggac atggcggtcc ctcgtaggca gccgaccaca
     2941 attcggtgta gtcctattaa catacgtgaa cataagattg taacagcggc cccgcagggc
     3001 cgatgtcctg gtgcgctggt ggcgacgcgg gcctttgacc agcgaccagc cgcaagcatc
     3061 caggcccgcg aggcgcgtcg gactgggcag gagttggggg gaagtttagc gcaaaatggg
     3121 acgcagaaaa atgaagcagc ttagcgctaa acggccgggc ggcaggccag agccccttcg
     3181 cctggcgttg gtgattacgg aactggagcc cggcggcgca gagcgctgcc tggtgaatct
     3241 ggcggtgcgg ctggaccggc agcggtttgc gccggtggtc tattcgctgg gccggaggcc
     3301 agagccgccg aaggacctgc tcgtgggcca acttgagcag gccggcgtgc cggtgcagtt
     3361 tttggatctg cgtcactggt cgcagtatgc ggcgggcgcg cggcggctgg cggctttgat
     3421 ggcccagcag cgggccgaag tggtgcagac gttcttgttt cacgcgaacg tcctcggctg
     3481 ccgggcggcg cggcgggcgg gagtgcaaca tgtcttcact ggcgtacgcg tggccgatcc
     3541 gcggcgctgg cgcacagcgc tggagcggtt ggcaacggga gcggcggaaa agattgtctg
     3601 cgtgagccag ggcgtcgccg atttctgccg cagtcgcgga tttgcagcac agaagctggt
     3661 cgtgattccc aatggcgtcg atgtcgatcg atttcagcga gctgccgcga ttgacttgac
     3721 gcagttcggc gtgcgggcgg gccggcgggc gatccttttc gtcggccggc tggaccggca
     3781 gaaggggctc gatgagttgc tcggcatgct gccgcgcgtg ttcgagcagg ttgccgactg
     3841 cgatctattg ctggtcggag atgggccgga gcgtgcggac ctgcagcggg cggcacaggt
     3901 atctggcatc caaggccgcg tccagtttgc cggctggcag gcggatgtgc cgggaatact
     3961 ggccgcctcg gagctgctcg tgctgccctc ccgctgggaa ggcatgccca acgtaattct
     4021 cgaagcgatg gccgcgggta agccggtggt ggcggcgaac gtggagggcg ttgccgagtt
     4081 gctgggcgcc ctgacggccg agcaaacggc tcacgccggt gacaagtctg cgctcgcgtc
     4141 gcggctcgcc gtcctcctcg ctaacccgcc tcgtgccaag gagttaggat cgctcaacca
     4201 gcaccgggcg atggccgaat tcagccttga tcgaatggtc caaaaattct ccgacctgtt
     4261 ccaagggagg tttgaggctt agcgggaaaa attcttcctt gcggcggcaa tttggtcatt
     4321 gtgcccttta tttctggcga attgcgccag gaaaggaagc tccccacgcc gctacggtcg
     4381 ccgggttttg cccaggtgcg attgacgaat ggacgagaat gtttacgttg tggggccgtg
     4441 tggtgagtcg tggggaaaag tgggacatga tgcttacagg aacctacgaa cgggcactcg
     4501 acgccaagct tcggctggcg atgcccaaac ggttccgtga ggcgctggcc gggcagtcgc
     4561 cgctggtgct cacgccggga accgatggct cactggccct gtttgctccc gcagctttcg
     4621 ctgccctcac ccagcgactc gcatcccgct ccccaaccgg acaggatgtc cgcgccttca
     4681 gccgcctgct gtat
//
LOCUS       DASUGB010000279         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1698933, whole genome shotgun sequence.
ACCESSION   DASUGB010000279 DASUGB010000000
VERSION     DASUGB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698933"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3077)
                     /locus_tag="VFV87_05365"
     CDS             complement(<1..>3077)
                     /locus_tag="VFV87_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103216.1"
                     /translation="DKVRFLTAIRDLQVNPASAAAALALLENAPVDPAAKMPRLLELQ
                     RLTRSVGNEWYDWDRLAPFAQTAVGKQDFVSATVLATGMLANIPNVDEPRKKAVRDLV
                     TQSLTRLGGVGLTIDESSPLAPLMQAALYFRLGDERLAFDGYLANKQLFDENRNQLPP
                     DLLTFVCDRLIAGGGDANHDYVEEVLRGWLVQHSESMQQDDATKAQMQLLLAKNFFKA
                     QRFDVARAEYTTVVNRYAATPQAVEAEFGIGETFLAQKVYDQAELVFEKLARSPLVDV
                     VVRAEFLRGVLAFRRGDREEARDIFRAVLERVPNVELANQALYNLAEVYGAEERYIDQ
                     LNLLRTVGRLGRNSKRRHTPGLPLSIVVHDSDLGISRGHNRIPVRVTTEPGGDTEMVY
                     LVGSGAGKGLFRADVETRLGQTTPGDNVLQLTGNDTIKCDYPDEFKAEFKSVPLSDVE
                     IRVAADAKFEIASSKIEDIQAETLSQQLEREAREEAADRRQSQVRPANQIKPGNLIYL
                     RVKDGDRDLTGEVDNVVIKLTADSGDQIQVALQETGPHTGIFEGTAQTGDLPAGALAS
                     DTAIDHSPLMSIDRDPKTYWMSEPDGATPKSLTIDMKDLLPVSRVKFETPNPAKYAPV
                     RGELFGSQDGQFWFRIAGNPDREPAAKLAAEYGQMTRRVYTGNHTDFSTWDQVVAQSK
                     NAKPIEEGPAEQLEWVRPEGNDESPKPFAVHWQGKFIHPKAGAVRFRVTGGKTALQLD
                     GMLELPLGPGGRSVDVWLDAGPHDLAIFAASAAGNQPVEAQLVRADQNSALVQLHPFR
                     AADFDLTSPAAQKPAATAVDATTGTIPLLIQSAKINKTTEQFGIHKIAENVEVIGYWQ
                     STEDWLEWQFDAPAAGPYEVWFEWGRDSEGPKFRAEIAGKVLETKIPSTGSYQTMRQA
                     RLGTVVIDAAGKHTFVLKAADAPGGGLWNLKGVSLKPAAGASVILEPTAWEFRFPQHE
                     LRFVRLLCHEYLGEALAIGNVEVAGEKADKLYIPTQEDVLALAANETLEIAAG"
BASE COUNT          491 a          976 c         1018 g          592 t
ORIGIN      
        1 cccggcggca atttccagcg tctcattcgc cgccaaagcg agcacgtctt cctgcgtcgg
       61 gatgtacagc ttgtcagcct tctcgccggc gacttcgaca ttgccgatcg ccagcgcttc
      121 gcccaaatac tcatggcaca agagccgcac gaaccgcagc tcatgctgcg ggaagcggaa
      181 ctcccaagcc gtcggctcca ggatgacgct ggcgccggcg gccggtttaa gcgaaacgcc
      241 cttcaggttc cagagcccgc cgccgggggc atcggcggcc ttgagcacaa acgtgtgctt
      301 gccggcggcg tcgatgacca ccgtgccaag ccgggcctga cgcatcgtct ggtagctgcc
      361 agtcgaggga atcttggttt cgagcacctt gccggcgatt tcggcccgga acttggggcc
      421 ttcgctgtct cggccccatt cgaaccagac ctcatacggc ccggcggcgg gggcgtcgaa
      481 ttgccattcc agccagtctt cggtcgattg ccagtagccg atcacttcga cgttctcggc
      541 gatcttgtgg atgccgaatt gttcggttgt cttgttgatc ttcgcgctct gaatgagcag
      601 tgggatcgtg ccggttgtgg catcgacggc tgttgccgct ggcttttggg cagcggggct
      661 ggtcaggtcg aaatcggcgg cccggaaagg atgaagctgg acgagagcgg agttctggtc
      721 cgctcgcacg agctgtgcct caaccggctg attgccggcg gcggaggcgg cgaaaatcgc
      781 caggtcgtgc ggaccagcgt cgagccacac atcgacgctc cggccgcccg gtcccagcgg
      841 cagctccagc atgccatcga gctgcagcgc cgtcttaccg ccggtcacgc ggaagcgcac
      901 cgcgccggct ttgggatgga tgaacttgcc ctgccagtgg acggcgaacg gcttgggtga
      961 ttcatcgttc ccttcgggac ggacccactc gagctgctcg gccgggcctt cctcgatcgg
     1021 cttggcattc ttcgattggg cgacgacctg gtcccaggtc gaaaagtcgg tgtgattgcc
     1081 cgtatagacg cggcgcgtca tctggccgta ttcagccgcc agtttcgcgg ccggctcgcg
     1141 gtcgggattg ccggcgatgc ggaaccagaa ctgaccgtcc tgactgccga atagctcgcc
     1201 gcgaacgggg gcgtacttcg ccgggttggg cgtctcaaac ttcacgcgag agaccggcaa
     1261 gaggtccttc atgtcgatcg tcagcgactt cggcgtcgcc ccatccggtt cgctcatcca
     1321 gtacgtcttg ggatcgcggt cgatcgacat cagcgggcta tggtcgatgg ccgtatcgct
     1381 ggccagggcc ccagccggaa gatcgcccgt ctgggccgtg ccttcgaaga taccggtgtg
     1441 cgggccggtc tcctggaggg cgacctgaat ctgatcgccg ctgtcggcgg tgagcttgat
     1501 gacgacgtta tcgacctcgc cggtcagatc acggtcgccg tctttcacgc gcaggtagat
     1561 gagattgccc ggcttgatct ggttggcggg gcgaacctgc gactgccggc ggtcggccgc
     1621 ctcttcgcgc gcctcgcgct caagctgctg gctgagcgtt tcagcctgaa tatcctcgat
     1681 cttgctgctg gcgatttcga acttcgcgtc ggcggccacg cggatttcga cgtcggacag
     1741 cggtacgctt ttgaactcgg ctttgaattc gtcgggatag tcgcacttga tcgtgtcgtt
     1801 gccggtgagc tgcaggacat tgtcgcccgg tgtcgtctgg cccaggcgcg tctcgacgtc
     1861 ggcgcggaac agccccttgc ccgccccaga gccgaccaga tagaccatct cggtatcgcc
     1921 gcccggctcg gtcgtcaccc ggaccggaat gcggttatgc ccgcggctga tgccgaggtc
     1981 gctgtcgtga acgacgatcg acagcggcag gcccggcgta tgccggcgct tgctattgcg
     2041 acccaagcgg ccgaccgtcc ggagcaggtt gagctggtcg atgtaccgct cttcggcgcc
     2101 atagacttcg gccaggttgt agagggcctg gttggccagt tcgacattcg gcacgcgctc
     2161 cagcaccgcg cggaagatat cgcgggcctc ttcgcggtcg ccgcggcgga aggcaagcac
     2221 gccgcgcagg aactcggccc gcaccaccac atcgacaagc gggctgcggg ccagcttctc
     2281 gaacaccaac tccgcctggt catagacctt ttgagccagg aacgtctcgc cgatgccgaa
     2341 ctcggcttcg acggcctggg gggtggcggc gtaacggttg acaaccgtcg tgtattccgc
     2401 gcgggccacg tcgaaccgct gggccttgaa gaagttcttg gcgagcaaaa gctgcatttg
     2461 ggccttggtc gcgtcgtcct gctgcatcga ttcgctgtgc tgcaccagcc agccgcggag
     2521 gacttcctcg acgtaatcgt ggttcgcgtc gccgccgccg gcgatcagcc gatcgcagac
     2581 gaacgtgagc aggtcgggtg gaagctggtt gcggttctcg tcgaagagct gcttgttggc
     2641 caaatagccg tcgaacgcca gccgctcgtc gccgagacgg aaatagaggg cggcctgcat
     2701 cagcggagca agcgggctcg attcgtcgat cgtcaggccg acgccgccga gccgcgtgag
     2761 cgactgcgtc accaagtcgc ggacggcttt cttccgcggc tcatcgacgt tggggatgtt
     2821 ggcgagcatg ccggtggcga gcaccgtcgc ggagacgaaa tcctgcttgc cgacagccgt
     2881 ctgagcgaac ggcgcgaggc gatcccagtc gtaccactca ttgccaaccg accgtgtcaa
     2941 tcgctgcagc tccaagagcc gcggcatttt cgcggctgga tcgacgggcg cgttttccag
     3001 gagagcgagc gccgcggccg ccgaggcggg gttgacttgc aagtcgcgaa tcgccgtcag
     3061 gaagcggacc ttgtcct
//
LOCUS       DASUGB010000280         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_586709, whole genome shotgun sequence.
ACCESSION   DASUGB010000280 DASUGB010000000
VERSION     DASUGB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586709"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VFV87_05370"
     CDS             complement(<1..1258)
                     /locus_tag="VFV87_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5103217.1"
                     /translation="MTASKKQIANGGTRAMTLDEQGPPHDSNAEAAVLSALVCLGHMD
                     DSAEVVNLKPDDFFQPQHRAIFDAMKELVDAGKPIDGLLLAAHLKARGNWEAIGAAAG
                     FSRIINAVPNVHFIRHYAELVCDKSRERENYRFALRIAEEVKDGLPWSDVLGTIQQHT
                     ERMEAQHLGSGEVELLSLADLVSGDFSTSYHVPGILAQGQPAIVAAPLKALKTSVCMD
                     LCLSLASGRHFLGKWPVNQTNTLFLSGESGKGALQQLATRICASKGIGDLEAITGFQM
                     SEWLPRFDAMADLAKLRRTIIRAGAGIVFIDPIYLCIDGDDANNLFAMGKQLRGVAKV
                     AKDTGTTLVLVHHFKKTLPDMYSQPELAWLSWSGFAEFARQWLLLNRRETYESGSGDH
                     RLWLTAGGSAGHGGAWGLDVSEGVISD"
     gene            complement(1291..1530)
                     /locus_tag="VFV87_05375"
     CDS             complement(1291..1530)
                     /locus_tag="VFV87_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5103218.1"
                     /translation="MVATVQPDASAREETLLLDERTTARLLGVSSRTLWTLSAAGEIP
                     FVRIGRRKLYSRETLVNWIRTREAEGKSQQRQTKQ"
     gene            complement(1710..2165)
                     /locus_tag="VFV87_05380"
     CDS             complement(1710..2165)
                     /locus_tag="VFV87_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103219.1"
                     /translation="MIDELAANVRRLGVPLLGLPDVPRMNTRERIRLSTDKFDEHWRQ
                     RHRDFHAAIDFMQSAIQSVTGITTHQHAPASAAVPSKRGRRGPEAVYDYSEDVKLLQD
                     WQAAKRQGASRKEFCRERNRQVKELTRAQTRHRARQQKQKRSNSRVKSH"
BASE COUNT          426 a          741 c          687 g          518 t
ORIGIN      
        1 tgtcggagat gacgccttcg ctcacgtcta atccccaggc cccgccgtgc ccagcgctgc
       61 cgccagctgt gagccacagt cgatgatcgc cgctgccgct ttcgtatgtc tcgcgtcgat
      121 tcaggagcag ccactgcctc gcgaattccg cgaagccact ccagctcagc caggccaatt
      181 ccggctggct gtacatgtcg ggaagagtct tcttgaagtg gtggacgaga acaagcgtgg
      241 tcccagtgtc ctttgcgacc ttggccacgc cgcgcagttg cttgcccatt gcgaacagat
      301 tgttcgcgtc gtcgccgtca atgcaaaggt aaatggggtc gatgaaaact atgccagcgc
      361 ctgcgcggat aatcgtgcgg cggagtttcg ccaggtctgc catcgcatcg aaccgcggca
      421 gccattcgga catttggaag ccagtgattg cttccaagtc gccgattccc ttgctggcac
      481 agatcctcgt ggccaattgc tgtagcgcgc ccttgccgct ctcgcctgac agaaacagag
      541 tattcgtctg attaacgggc cacttgccga ggaagtgccg cccgctggcc agcgacaagc
      601 acagatccat gcaaacggat gtctttagcg ccttcagcgg ggcggccacg attgccggct
      661 ggccctgcgc gagaatgcca ggaacgtggt acgacgtgct gaaatcgccg gagaccaaat
      721 cggccagcga cagcaactcg acttccccgc tgcctaggtg ctgcgcttcc atccgctccg
      781 tgtgctgctg aatcgtgccc aacacgtcgg accagggcag gccgtctttg acttcctccg
      841 cgatacgaag cgcaaaccga tagttctccc gctcgcggga cttgtcgcaa accaattcgg
      901 catagtggcg gatgaagtga acgttcggga cggcgttgat gatgcgagag aagccggcgg
      961 ccgcgccgat cgcttcccaa ttgcctctcg ccttcagatg cgcggcgagc aacagcccgt
     1021 cgatcggctt gccggcatcc accagctcct tcatcgcgtc gaaaatcgcc cgatgctggg
     1081 gctgaaaaaa atcgtcgggc tttaggttga cgacttccgc agaatcgtcc atgtgcccga
     1141 ggcaaacgag cgccgataga actgcagcct cggcgttcga atcgtgaggc ggcccctgct
     1201 cgtcgagggt catcgcgcgg gtgccgccgt ttgcaatctg cttcttgctg gcggtcatcg
     1261 cggcagcact cccaacttga tccgcgtggc ctactgcttg gtttgccgct gctggctctt
     1321 cccctcggct tcccgggtgc gaatccagtt aaccagcgtt tcgcgagaat acagcttgcg
     1381 ccggccaatt cggacgaacg ggatttcgcc cgcggccgaa agcgtccaca gagtacgcga
     1441 cgataccccg agcagccgcg cggtcgttcg ttcatcgagc agcagcgttt cttctctggc
     1501 cgaagcatcg ggctgaactg ttgcaaccat gatcgatcaa gctccttcgg ttgtcaggcg
     1561 accaattcgg tgtcggcggc acgttcgcaa ttatccccca cgttgcgcag gcgcagggat
     1621 tcgggcgaaa tcgctgcgct ccgcccacgg tctattcagc aagggatttc acggcctagc
     1681 atcgtgccgc aagaaaatgg cgcagcgttt caatggctct tgactcggga attactgcgc
     1741 ttctgctttt gctggcgtgc ccggtgacgt gtctgcgctc gggtcaattc cttcacctgc
     1801 ctgtttctct ccctgcagaa ttccttgcga cttgcacctt gtcgcttcgc agcttgccaa
     1861 tcttgcagta gctttacatc ctcgctgtaa tcgtaaactg cttccggtcc cctccgccct
     1921 cgtttgctcg gaacggctgc agacgcgggc gcatgttgat gagttgtaat accggtcacg
     1981 gattgaatcg ccgactgcat gaagtcgatt gcagcatgaa agtcccgatg gcgttgccgc
     2041 caatgttcgt cgaatttgtc tgtcgacaag cggatgcgct cgcgggtgtt cattcgtgga
     2101 acatccggta gtcccaagag gggtacgccc aagcgacgta cgtttgcggc cagctcgtcg
     2161 atcaccttgc ctggatcgta tggttgatcg tcgattgccg catgtcggcg cctgagtttg
     2221 tggattcgcg ccaatagcgc tcgcgcatcc atctgactgc ctttcgtcac cgggccgggc
     2281 cgggcgacgg ctgcgaaagg cataaggcaa ccgccgccca gtccagattc ggccgcgtgt
     2341 agctactcac gcttggccac ccggtatggc tt
//
LOCUS       DASUGB010000281         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1143065, whole genome shotgun sequence.
ACCESSION   DASUGB010000281 DASUGB010000000
VERSION     DASUGB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143065"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFV87_05385"
     CDS             complement(<1..464)
                     /locus_tag="VFV87_05385"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5103220.1"
                     /translation="MPDSPLDLKLFIRDIPDFPKPGILFRDITPLLASPDALRETVRR
                     MAEPFRDEKIHAVVAAEARGFIFAAPMAIELGAGFVPVRKPGKLPFDRHSFRYDLEYG
                     SDTLEMHVDGVPPGAKVLVVDDLLATGGTVGACVKLLEKTGARIAGCAFAIEL"
     gene            complement(545..1264)
                     /locus_tag="VFV87_05390"
     CDS             complement(545..1264)
                     /locus_tag="VFV87_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX5103221.1"
                     /translation="MKLLDLTLPTPTENLALDQALLDAAEAGELEDEVLRLWEPAQPV
                     VVVGRGSRVEEEVDVAACRSAGVPILRRDSGGAAIVTGPGCLMYGVVLRYEARQHLRM
                     IDEAHRHVLGIVARAISRLVPSAAHCGTSDLAIGDRKFSGNSLRCKREHLLYHGTLLY
                     DFDLPLIGGLLRMPPRQPAYRAGRRHTDFVVNLPASREQLEQALCSVFAADQRLESWP
                     DELTQRLVKERYALDAWHLAR"
     gene            complement(1266..1703)
                     /locus_tag="VFV87_05395"
     CDS             complement(1266..1703)
                     /locus_tag="VFV87_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103222.1"
                     /translation="MATPANPYSATHEELQPATPPAHRAWIGIGLIVGTLVLLAAVVV
                     TAVLAFVAGAVVGTSDVYHQRATRQADQIRIYLGSQEERYADVTIHEASNGWSYLEGS
                     VDSQEDFDQLHDELKRLFGEELAEDMMRLVEIKEQAVPPAAAD"
     gene            1784..>2766
                     /locus_tag="VFV87_05400"
     CDS             1784..>2766
                     /locus_tag="VFV87_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5103223.1"
                     /translation="MSASQADFPAKPPKPPPGEMLPPVEAPTGTFILQLFLIPLLIVT
                     IVVVLWLSFGWLAQMGRDNPDALVKSIERGDNASWQRAYELADLLRSPDPRYDAVRRD
                     NALAERLADFLQRDMQEPAQGKEDQSRVIRRMYLCRALGGFEVLGGLQALMQAAEQER
                     DPVEVEVRYSAIEAIATLAHNCGPEILQNDKAVIDVLLKASREQDDASTPPTLAADGS
                     EVLFRPHAELRAVAAYALGVIGGEDARDRLQKMLIDGYPNARYNAATGLARHGDIACE
                     GVLREMLDPDQDLAVRDERYDREKDRKRATVLLNGIKATLTLAEANPEADLT"
BASE COUNT          493 a          939 c          890 g          444 t
ORIGIN      
        1 agctcgatgg cgaacgcaca gccggcgatc cgggcgcccg tcttctccag cagcttgacg
       61 cacgcgccga cggtgccgcc ggtcgccagc aggtcatcga ccaccagcac tttcgccccg
      121 ggcggcacgc catcgacgtg catttccagc gtatcgctgc cgtattccag gtcgtagcga
      181 aatgagtgcc ggtcgaaggg taacttgccc ggcttgcgaa cgggaacgaa tcccgcgcca
      241 agctcgatcg ccattggtgc agcgaagata aacccgcggg cttccgcagc aactaccgcg
      301 tggattttct cgtcgcgaaa tggctccgcc attcgccgca ccgtctcgcg cagcgcgtca
      361 ggcgaggcca acagcggcgt gatgtcgcgg aacaggattc ccggcttggg aaagtccggg
      421 atgtcgcgga tgaagagctt gaggtcgagt ggggagtcgg gcatggatgt gttgcgacaa
      481 taagagcgcg aggcggggtg aatatacagg agtcaggagt caggggtcag gggtcagggg
      541 gccactatcg cgccagatgc cacgcatcga gcgcgtagcg ctctttcacc agccgctgag
      601 tcagctcgtc aggccacgat tccagccgct ggtcggcagc gaacacactg cacaacgcct
      661 gttcgagttg ttcgcggctt gccggcaaat tcacgacaaa gtcagtgtgc cgccggccgg
      721 cgcgatacgc tggctggcgc ggcggcatac gcaatagccc tccgatcaag ggcaggtcga
      781 agtcgtacag cagcgtgccg tgatagagca ggtgctcgcg cttacagcgg aggctgttgc
      841 cggagaactt gcggtccccg atggccaggt cgctcgtgcc gcaatgggcg gcgctgggaa
      901 cgagcctcga aatcgcccgg gcaacgattc ccagcacgtg ccgatgggcc tcgtcgatca
      961 tccgcagatg ctgccgcgct tcgtatcgca ggacgacgcc atacatcaag cagccgggac
     1021 cggtgacgat tgccgccccg ccgctgtcgc gccgcaagat cggcacgccc gccgagcggc
     1081 aagcggcgac atccacctct tcctcaacgc gcgacccgcg gccgacgacg accaccggct
     1141 gcgccggctc ccagagccgc agcacttcgt cctcgagctc gcccgcttct gcggcgtcga
     1201 ggagggcctg gtcgagggcc agattctcgg ttggcgtcgg cagcgtcagg tcgagcagct
     1261 tcatgtcagt ccgccgcagc cggcgggacc gcctgctcct tgatttcgac cagccgcatc
     1321 atgtcttcgg ccagctcttc gccgaacagt cgcttgagct cgtcgtgaag ctggtcgaaa
     1381 tcctcctgcg aatcgaccga gccctccaga tatgaccagc cgttggaggc ctcgtgaatc
     1441 gtcacgtcgg cgtatcgctc ttcctggctg cccaggtaga ttcggatttg atccgcctgc
     1501 cgcgtggccc gctggtggta gacgtccgac gttcccacga cggcccccgc cacgaacgcc
     1561 agcaccgcgg tgaccaccac cgcggccagc agcacgagcg tcccgacaat cagcccgatc
     1621 ccgatccagg cgcgatgagc gggcggcgtc gccggctgca gctcttcgtg cgtggcggaa
     1681 tacggattgg ctggggtcgc catgaattgc cggctttcac cgcccttgga cgcctcggtc
     1741 gagcaaccta aactgaagat aaaccacctg ccgcaagccc gccatgtccg ccagccaggc
     1801 cgattttccc gccaaaccgc ccaagcctcc gcccggcgag atgctgccgc cggtcgaagc
     1861 gccgaccggc accttcattc tgcagctctt cctcatcccg ctgttgatcg tgacgatcgt
     1921 ggtggtcctc tggctctcgt tcggctggct ggcccagatg gggcgcgaca atccggacgc
     1981 cttggtaaag tcgatcgagc gcggggacaa cgcgagctgg cagcgggcct atgagctggc
     2041 cgatctgctc cgcagccccg atccgcggta cgacgccgtc cgccgcgaca acgctctggc
     2101 cgagcgcctg gccgatttcc tccagcgcga tatgcaagag cccgcccagg gcaaagaaga
     2161 ccaaagccgc gtgatccggc ggatgtacct gtgccgggcg ctgggcggat tcgaggtcct
     2221 gggcggcttg caggcgctca tgcaggcagc cgagcaggag cgcgacccgg tggaggtcga
     2281 agtccgctat agcgcaatcg aagcgatcgc cacgctggcc cacaactgcg gccctgagat
     2341 tctgcaaaac gacaaggccg tgatcgacgt gctgctcaag gcctcacgcg agcaggacga
     2401 tgcttccact ccgccaacgc ttgccgcgga cggctcggag gtccttttcc ggccgcatgc
     2461 cgagctgcgc gcggtcgccg cgtatgcgct cggcgtgatc ggcggcgaag acgctcggga
     2521 ccggctgcag aaaatgctga tcgacggcta tccgaacgcc cgctacaacg ccgcgaccgg
     2581 cctggcgcgg cacggcgaca tcgcctgcga gggcgtcctc cgcgaaatgc tcgatcccga
     2641 tcaggatctg gccgtccgcg acgagcgcta cgaccgcgaa aaggaccgca agcgggccac
     2701 cgtgctgctc aacggcatca aggccacgct gacgctggcc gaagccaatc cagaggccga
     2761 tctgac
//
LOCUS       DASUGB010000282         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1281972, whole genome shotgun sequence.
ACCESSION   DASUGB010000282 DASUGB010000000
VERSION     DASUGB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281972"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..666)
                     /locus_tag="VFV87_05405"
     CDS             complement(331..666)
                     /locus_tag="VFV87_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103224.1"
                     /translation="MTFEIQCSIWLASKTRWAHLTKLVRLASVPRVGEYLKFQNHEQG
                     DYFAWRVSQVTYRESGEIEVWTELLDNIDDRGYSFDEESEFDSYFQSYVGEGWTCERG
                     IKPNTRCKA"
     gene            complement(679..2100)
                     /locus_tag="VFV87_05410"
     CDS             complement(679..2100)
                     /locus_tag="VFV87_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5103225.1"
                     /translation="MPRPQKIAYLTAGAAGMICGSCLHDNTLARSLIELGHDVQLVPL
                     YTPIRTDEQDVTVHEIFYGGINMYLQQKVPLFRWLPKWLDHWLDQPWLINWAAGRSVK
                     IDPKQVADITLSILRGREGFQRKEVERLVDWLTADGPPDVIVFSNILTAGCVPEIKRR
                     IDVPVVVTLQGDDIFLRGLPEPHHTQALAEIRRLAEQIDGYIAHTHFYAGAMAEYLGL
                     ARERIQIAPLGIDVRDFEEGFRVQGSGDRRQETGDREQEPDSNPKSKIQNPKFARPPT
                     VGYLARLAPEKGLHILAEAFLRLRQMPEMEQAKLRIAGWMGKANKAYADEVFAKLREA
                     GLGEAFEYVGEVDRRGKLDFLASIDVLAVPTTYRDPKGLFVLEALAAGVPVVQPEHGS
                     FPEMLGELAGGLLHRPEDPQHLAERLHLLLTDEVQWIRLASSGRQNVHTYRNAQVMAQ
                     RTIEALEQIMNTATLSREQTCSR"
     gene            complement(2197..2775)
                     /locus_tag="VFV87_05415"
     CDS             complement(2197..2775)
                     /locus_tag="VFV87_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103226.1"
                     /translation="MLGHLLTSWTIRLALALYVAWLAGWLVASGPRWAAASRWIWTLA
                     CGLFLVHVACAFHFYHHWSHAAAWQDTADETQALLGVAFGDGIYFSYAFMVLWVLDVA
                     WLWLVSPVWTPWPRLLVHAFLLFIAFNGAIVFEAGPTRWFGLAACAVLAGLAVRRAYN
                     WQRSIRRGQKTEDRGQTAEEDRGADSVLSTEY"
     gene            3304..3759
                     /locus_tag="VFV87_05420"
     CDS             3304..3759
                     /locus_tag="VFV87_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX5103227.1"
                     /translation="MKRTDMLKQTQKQLVARRDALRRSLEGDRRMLQSLSESGVGDEI
                     DAAIATEQAEMDSQMASFESRELARIEHALEQIREGRYGRCEVCEKAIAPVRLKALPY
                     ATECIQCASKGERRGASASGFRPVNRIAAFATDDDREPSIDDLELEESR"
BASE COUNT          739 a         1126 c         1293 g          628 t
ORIGIN      
        1 gggttccttg gattttggag gcataactcc ggtgtccaat gggagttgga acagaacggt
       61 cgtttcgagt accctgggcc tgcccggaga aacggagtca ggctctgctg ggaagcaacc
      121 tgacaaggga tatccgggcc gacggtgcgc caatggtgca gccgggcccc tggcggcaac
      181 tgttgccagg ggccgctttc gtcaaatccc attggacacc ggagtcatgc cttagaaaat
      241 cctgcctccg aagcccgtcc gccgcaacga cctgccgccg cgcacgcttc actcggatga
      301 gccgcgaaat ctacaaaact gcggatgatg ctacgccttg caacgcgtgt tgggcttaat
      361 cccacgctca caagtccagc cctcgccgac gtaggactgg aagtagctgt cgaactccga
      421 ttcctcgtcg aaggagtagc ctcgatcgtc gatgttgtcg agcagttcgg tccagacctc
      481 gatctcgccc gattcgcgat acgtgacctg cgacacgcgc caggcgaaat agtcgccctg
      541 ctcgtggttc tgaaacttca agtactcgcc gacgcgcggc acactcgcca ggcgaaccag
      601 ctttgtgagg tgcgcccagc gagtcttgct cgccagccag atgctgcatt gaatctcgaa
      661 tgtcatgcgt cgtccgcttt accgggagca ggtttgctcc cggctaagag tggcggtatt
      721 catgatctgc tccagggctt cgatcgtgcg ctgggccatg acttgggcgt tgcggtaggt
      781 gtggacgttc tgccggccgc tgctggccag gcggatccac tgcacttcgt cggtgagcaa
      841 caggtgcagc cgctcggcca ggtgctgcgg gtcttcgggc cggtgcaaga ggccgccggc
      901 cagttcgccc agcatctcgg ggaacgagcc gtgctccggc tggacgacgg gaacgccggc
      961 ggcaagggct tccaggacga agagcccctt ggggtcgcgg taggtcgtgg gcacggccag
     1021 cacgtcgatc gaggccagaa aatcgagctt gccgcggcga tcgacctcgc cgacgtactc
     1081 gaacgcctcg cccagaccgg cctcgcgcag cttggcgaag acctcgtcgg catacgcctt
     1141 gttcgctttc cccatccagc cggcgatccg cagcttcgcc tgctccatct ccggcatctg
     1201 ccgcaggcgg aggaaggctt cggccaggat gtgcagccct ttttccgggg cgagacgggc
     1261 gaggtagccg acggtggggg ggcgggcaaa ttttggattt tggattttgg attttggatt
     1321 ggaatccggc tcctgttccc tgtcccctgt ctcctgtctc ctgtctcctg aaccctgaac
     1381 cctgaaccct tcctcaaagt cccgcacatc aatccccagc ggggcgatct ggattcgctc
     1441 gcgggcgagg ccgaggtatt cggccatcgc gccggcgtag aagtgcgtgt gggcaatata
     1501 gccgtcgatc tgctcggcca ggcggcggat ttcggccagg gcctgggtgt ggtgcggctc
     1561 cgggaggccg cgaaggaaga tgtcgtcgcc ttgcagcgtg acgacgaccg gcacgtcgat
     1621 ccgccgcttg atctccggca cgcacccggc ggtgaggata ttgctgaaga cgatcacgtc
     1681 gggcgggccg tcggccgtca gccaatcgac tagccgctcg acttctttcc gctgaaagcc
     1741 ctcgcgtccg cggaggatgg agagggtgat gtcggcgacc tgcttcggat cgatcttgac
     1801 gctgcggccg gcggcccagt tgatgagcca cggctgatcg agccaatgat ccagccattt
     1861 tggcagccag cggaagaggg ggaccttctg ctggaggtac atgttgatgc cgccgtagaa
     1921 gatttcgtgg acggtgacgt cctgttcgtc ggtgcggatc ggggtataga gcgggacgag
     1981 ctgcacgtcg tgtcccagct cgataaggga gcgggcgagg gtgttgtcgt gcaggcagct
     2041 cccgcagatc atgcccgccg ccccggcggt gaggtacgcg attttttggg ggcgaggcat
     2101 ggggggagag ggcggggaga ggacagagga cagaggacag gggacaggag ataggggacg
     2161 ggggacagag gccgcttgga cgttgatgat gagtactcag tattcagtac tgagcaccga
     2221 gtccgcccct ctgtcctctt ctgccgtctg tcctctgtcc tctgtcttct gtcctctcct
     2281 gatcgaacgc tgccaattat aggcccgccg aacggccagg cccgccagca ccgcgcaggc
     2341 agccagcccg aaccagcggg tggggccggc ttcgaagacg atggctccgt tgaaggcgat
     2401 gaacaacaaa aaagcgtgaa ccagcagccg gggccagggt gtccagaccg gcgagacgag
     2461 ccacagccac gccacgtcga gcacccagag gaccatgaac gcatagctga agtagatgcc
     2521 atcgccgaag gcgacgccca gcagcgcctg ggtctcgtcg gctgtgtcct gccaggcggc
     2581 ggcgtggctc cagtggtgat agaagtggaa cgcgcaggcc acgtggacca ggaacaggcc
     2641 gcaggcgaga gtccagatcc agcggctggc ggcggcccac cgcggcccgg aagcgaccag
     2701 ccagccggcc agccaggcga catacaacgc cagggccagc cgaatcgtcc aactggtgag
     2761 caagtgtccg agcatggagg cgggagatgg cgggatcgta ggcttctcgc ccgcgagaag
     2821 cagacgacgg gtttggcgcg caacgctcgg cgccagaacc agcggaatgc ctctcgcgga
     2881 gcgagaggcc tacgattctg ggcggggagc gcgaaggagc caagatcggc cccgccgcga
     2941 cgaagaccat tgtacacgcc gctgcgcgct gcgggcgaga agcaagcgga ggagtaagag
     3001 aaagagcagg attacgagta ggaggattaa gagcaggagc aagagtaaga gcaggaaaga
     3061 ccgcggcggc cagtgcgtgg gaatcaagat tttccctttt ggcagccggc gaaccggcac
     3121 aacgattgct gcgtacggag catgaaccca acgcagggtt ccggagatat ccgaaagatt
     3181 cggccaccga aaaccattca cggcagggag aaaacagcgc ccagcatcaa accctgataa
     3241 gcaggaaacc ctgatgagca ggtccaggcg gcgatttgtc gtctgcaccc gggaggaggc
     3301 accatgaaac ggacagacat gttgaagcag acccagaagc aattggtggc ccggcgcgac
     3361 gccttgcggc gctcgctgga aggagaccgg cggatgttac agagcctgag cgaatcgggc
     3421 gtgggcgacg agatcgacgc cgccatcgcc accgagcagg ccgagatgga ttcgcaaatg
     3481 gccagcttcg aatcgcgcga gctggcgcga atcgagcacg ccctggagca gattcgcgag
     3541 ggccgttacg gccgctgcga agtgtgcgag aaggcgatcg ccccggtgcg gctgaaggcg
     3601 ttgccctatg ccaccgagtg catccagtgc gcgagcaagg gcgagcgccg gggagcctcg
     3661 gccagcgggt ttcgccccgt gaaccggatt gcggccttcg cgaccgacga cgaccgcgag
     3721 ccgtcgatcg acgacctcga actcgaggaa agccgatagg cgcgaacggc ggcagggagg
     3781 ggtagc
//
LOCUS       DASUGB010000283         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_309735, whole genome shotgun sequence.
ACCESSION   DASUGB010000283 DASUGB010000000
VERSION     DASUGB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309735"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFV87_05425"
     CDS             complement(<1..544)
                     /locus_tag="VFV87_05425"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5103228.1"
                     /translation="MAKRRDTSLGNGVYYAANDYVGIGRRLLILLVDGIVLAFLLLNF
                     VVLWAIIRGGPPLIFTAAVLGITWIYTVLFKRSPLRTVGYWLTSCRLVNLQGERPSLF
                     ALTFRSVLWMFGPFNLLFDIFWSGLDDDRQTLRDRFAGTCLINHSAVPIGTGEIHLTY
                     FDIGTFNLMYPRVTHPKDAAA"
     gene            complement(590..2419)
                     /gene="lepA"
                     /locus_tag="VFV87_05430"
     CDS             complement(590..2419)
                     /gene="lepA"
                     /locus_tag="VFV87_05430"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5103229.1"
                     /translation="MSPTSHIRNFCIVAHIDHGKSTLADRLLEFTGTVTKREMQDQLL
                     DDMALERERGITIKARAVSMKYTRSPPAESLRGDQVYELNLIDTPGHVDFHYEVSRSL
                     ACCEGAILVVDAAQGVEAQTLANAFAAMNSGLFIVPFINKIDLSNARTDEVLLEMEHS
                     LAIDHNDVLKGSAKANLGIAELLDAVIDRIPPPKGDPSGVLQAMVFDSHYDDYRGAIT
                     YVRVMNGTVKKGQKVRFLSSGTTHDVVDLGQFVPGPRPCESLQAGQVGYLICNIKSLS
                     MVHIGDTISIPGDKAAAMLPGYKEPKRMVYCGLYPSDGQDFSELREALEKLSLNDPSF
                     EFEPESSEALGFGFRCGFLGLLHMEVVQQRLEGESDVDLVQTAPNVTYEIVLRTGETL
                     QIHKPQDVPDSGQIEEFRQPIVRVSLIQPVEYIGPVMKLCQDRRGTLVRQEYLSPTRT
                     MLVYDVPLAEVIYDLHDKLKSATRGYGTMDYELVGYETADLVRMDILVNNNRVDALAV
                     ICHRADAERRGRAVCKRLKEEIDRHMFEVAIQAAIGTRVLARETKPAMRKNVTAKCYG
                     GDITRKRKLWAKQREGKKRMKSIGQVDIPQKAFLAVLETGDEK"
BASE COUNT          477 a          738 c          828 g          520 t
ORIGIN      
        1 ctgctgccgc gtctttggga tgagtgacgc ggggatacat gagattgaac gttccgatgt
       61 caaagtacgt gagatggatt tcgccggtcc cgatgggaac ggcgctgtgg ttgatcaaac
      121 acgttcctgc gaaacgatcg cgaagagttt gccgatcgtc gtcgagacca gaccaaaaga
      181 tgtcgaacag cagattgaat ggtccaaaca tccagagcac ggaccggaag gtcagagcga
      241 agagcgaggg gcgctcgccc tgcaagttca caagccggca actggtaagc caatagccga
      301 cggttcgcag cggcgaacgc ttgaacagaa cggtatagat ccaagtgatc ccaaggactg
      361 ctgcggtgaa gatgagcggc ggtccgccgc gaatgatggc ccagaggacg acaaagttga
      421 gaagcaagaa cgcgagcacg attccatcca cgagcaagat gagcagccgg cgtccgatgc
      481 cgacgtaatc gtttgccgcg tagtaaacgc cattgccgag ggaggtgtcg cgccgctttg
      541 ccatgtgagg tcagcctcct cgacatgcgt ggatcaaaga cgcactttac tacttctcat
      601 cccccgtctc cagcaccgcc aggaatgctt tctgcgggat atcgacctgg ccgatcgatt
      661 tcatccgctt cttgccttcg cgctgcttgg cccagagctt ccgcttgcgg gtgatgtcgc
      721 cgccgtagca tttggcggtg acgtttttcc gcatggcggg cttggtttcg cgggccagca
      781 cgcgggtgcc gatggcggcc tggatggcga cttcgaacat gtggcggtcg atttcttctt
      841 tcagccgttt gcagacggcg cggccacggc gctcggcgtc ggcccggtgg cagatgaccg
      901 ctagggcatc gacgcggttg ttgttgacaa ggatgtccat ccgcacgagg tcggccgtct
      961 cgtagccgac cagctcgtaa tccatcgtgc cgtagccgcg ggtggcgctc ttcagcttgt
     1021 cgtgcaggtc gtagatcact tcggccagcg gcacgtcata gacgagcatc gtccgcgtcg
     1081 gcgagaggta ctcctgccgc accagcgtgc cgcggcgatc ctggcagagc ttcatcaccg
     1141 ggccgatgta ttcgaccggc tggatcaggc tgacgcggac gatcggctgg cggaattctt
     1201 cgatctggcc ggaatcggga acgtcttgcg gcttgtggat ttgcagcgtt tcgcccgtgc
     1261 gaagcacgat ttcatacgtc acgttcggcg cagtttgcac cagatcgacg tcggactcgc
     1321 cctccaatcg ctgctggacg acttccatgt gtaacaggcc gaggaacccg cagcgaaagc
     1381 caaagccgag ggcctcgctc gattcgggct cgaactcgaa gctggggtca ttgagcgaaa
     1441 gtttttccaa agcctcacgc agctcgctga agtcctggcc gtccgacgga tagaggccgc
     1501 aatagaccat gcgcttcggc tctttgtagc cgggcaacat cgccgcggcc ttgtcgccgg
     1561 gaatgctgat cgtgtcgccg atatggacca tgctcagcga tttgatgttg cagatcaaat
     1621 agccgacctg gccggcttgg agcgattcgc aggggcgggg gccggggacg aactggccga
     1681 gatcgaccac gtcgtgcgtc gttccgctgc tgaggaagcg gaccttctgg ccctttttca
     1741 cggtgccgtt catcacgcga acgtaggtga tcgcgccgcg atagtcgtcg tagtgcgagt
     1801 cgaagaccat cgcctgcagg acgccagacg ggtcgccctt cggcggcggg atgcggtcga
     1861 tgaccgcgtc cagcaattcg gcgatgccga ggttggcctt cgcgctccct ttgagcacat
     1921 cattgtgatc gattgccagg gagtgctcca tttcgaggag cacctcgtcc gtgcgggcat
     1981 tggagagatc gatcttgttg atgaacggca cgatgaagag gcccgagttc atcgccgcga
     2041 aagcgttggc cagcgtttgg gcttcgacgc cctgcgctgc atcgacgacg agaatcgctc
     2101 cctcgcagca cgccagcgag cgggagactt cgtagtgaaa atcgacgtgg ccgggcgtat
     2161 cgatcaggtt cagctcatag acctgatcgc cccgcagtga ttctgccggc gggctgcgcg
     2221 tgtacttcat cgagacggcg cgggctttga tcgtgatccc tcgctcgcgc tccagggcca
     2281 tgtcgtccaa gagctggtcc tgcatctcgc gcttcgtcac cgtgcccgtg aattccagca
     2341 gccggtccgc gagcgtgctc ttgccgtggt cgatatgagc cacgatgcag aagttgcgga
     2401 tgtgggaggt gggggacata ggcgaggagt caggggtcgg gagtcaggag tcagggatgg
     2461 aagtccgttc aactgactcc tgacacctga ttgctgactc cttatttctt ccggtgctgc
     2521 tccagccggg cgatgccggc ggcgccgatg aagccggcgt cgc
//
LOCUS       DASUGB010000284         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_586921, whole genome shotgun sequence.
ACCESSION   DASUGB010000284 DASUGB010000000
VERSION     DASUGB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586921"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFV87_05435"
     CDS             complement(<1..678)
                     /locus_tag="VFV87_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103230.1"
                     /translation="MPADRGFRTALIFTLVAAAFLLAIHGSRFVQTNDEGILLEPAQR
                     IAAGESPYVDFFGYMSPGSYWIQAVVFRLFGVSLVTGRIPVIVGFSLQCGILLWLVSR
                     LATLRAAILVTLFFFGLQIGDPSFLTAQHRWDSGTLAPLAVCFAYESHQRASLAWSAA
                     AGALLATAAWCTPSMALVIVPVAAWFAWLRNWRALAAVTAGIAFVSAAAVLLLIAEGS
                     LGAFVQQL"
     gene            complement(668..1390)
                     /locus_tag="VFV87_05440"
     CDS             complement(668..1390)
                     /locus_tag="VFV87_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5103231.1"
                     /translation="MSHAAQAAEYQYEGAELDLFEKAVNWKSYWSSRISASIRGDVLE
                     VGAGIGANTALFTTQRFDSWTCLEPDAALASRIALPAGNRHRSRIGTMASLDATDQFD
                     AILYIDVLEHIAEDATELIRAAGHLRNSGHLIVLSPAWPFLFSPFDAAIGHFRRYTRT
                     SLRAAARSAQLREKEVIYLDCAGMLASAANRLLLRSAMPTLDQVLLWDRRLVPVSRCI
                     DRLFGEHLGKTIVGVWEKSHAR"
     gene            complement(1387..2445)
                     /locus_tag="VFV87_05445"
     CDS             complement(1387..2445)
                     /locus_tag="VFV87_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5103232.1"
                     /translation="MASHSDSLVILIPNFNDWEPLQLLLNQLDTVAAGLGWCTSVLVV
                     DDASTLPMPANWPQTAPASLVPIEVLHLRANLGHQRAIALGLYHINETRPGATVVVMD
                     GDGEDRAADILALLAEYERSGRRDAIFAARAKRMESFVFQFFYQLYRLLHRALTGIEV
                     RVGNFSVLPPSALARLMAVSDLWNHYAAAVLRARLPRRLLPLARGNRLAGNSQMNFTG
                     LLVHGLSAISVFSDHVSARLLTASAVLGVAVTALATVATAAWSAGIALPDAVIYGLIT
                     LAAITVQSLLLTAFFVFLIAHRRATPGFLLPRDARHFILGVSVAGERSSLELLAAALH
                     SATVHPPATDQTATEVRK"
     gene            2520..3830
                     /locus_tag="VFV87_05450"
     CDS             2520..3830
                     /locus_tag="VFV87_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX5103233.1"
                     /translation="MLLRLVLALAFCASTQAEIRKLTILHTNDLHARVIPDDAGRGGF
                     AYVTNVLRQERKGCGHCIHLSAGDLVQGMPVSSIFRGIPVYEMANRMGIDALTLGNHE
                     FDYGWQQIERFRRTAKFPLLSANALDADGKSIGDKPYTVLKVNGLRVGVIGLLMGNLV
                     EGYLTAKTAGPVKIAPVVGAARAAANDLAGRADVIIALGHVLPAEGSAILKEVPEVAA
                     VVEGHSHRGLEEMEQLGGRVRVGLKGYGVEVGRLDLEVDTATHQAVSAHWKRIPVESR
                     KVPPAEDVAKVVAKWERKVAQVVDKPVGEARREFLQPDLKVLMEQAMCEEMGADFSHM
                     NRGGVRDKLPKGTIRERELWNIMPFDNTMMLARVKGRLLSDALRAGRTVEPQREYVVA
                     LADYSVENEAQRKELGIEGWTFERTDKQLRQLLIDWVKKKKVLE"
     gene            3858..3935
                     /locus_tag="VFV87_05455"
     tRNA            3858..3935
                     /locus_tag="VFV87_05455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3893..3895,aa:Pro,seq:ggg)
BASE COUNT          818 a         1039 c         1446 g          634 t
ORIGIN      
        1 cagttgctgg acaaaagcgc ccaggcttcc ttcggcgatg agcaggagta cggcggccgc
       61 gctgacgaag gcgatgccgg cggtgaccgc ggccaaagcg cgccagttcc ggagccaggc
      121 gaaccaggcc gccacgggaa cgatgaccag ggccatcgac ggagtgcacc aggcggctgt
      181 cgcgagcagc gcacccgcgg cggcagacca cgcgagcgag gctctctggt gagactcgta
      241 ggcgaagcag acggcgagag gcgcaagcgt gccgctgtcc caacgatgct gggcggtgag
      301 gaaggatgga tcgccgatct gcaaaccgaa gaagaacagt gtgaccagga tggcggcgcg
      361 caatgtcgcc aaccgagaaa cgagccaaag cagaatcccg cactggaggg agaatccgac
      421 gatgaccggg atgcgtcctg tgacgagcga gacgccgaaa aggcggaaca ccacagcctg
      481 aatccagtag ctgccagggc tcatgtatcc gaagaaatcg acatagggag actcgccggc
      541 ggcgattcgc tgagcaggtt ccaggagaat gccctcgtcg ttagtttgga cgaaacggct
      601 gccatgaatg gccagcagga atgcggcggc aactagcgtg aagatgagtg cggtgcgaaa
      661 gccccggtca gcgggcatgg gacttctccc acacgccgac gatggtcttg cccaggtgct
      721 cgccgaagag gcgatcgatg caccgggaga cgggcaccag acggcggtcc cagagaagaa
      781 cctgatcgag cgtgggcatc gcgctgcgga gcaggagccg gtttgcggcc gaggcgagca
      841 tgccggcgca atcgaggtag atcacttcct tttcgcggag ttgcgcagag cgcgccgcgg
      901 cacgcaacga cgtgcgggtg tagcggcgga agtggccaat ggccgcatca aacggagaga
      961 agaggaaggg ccacgcgggc gagagcacga tcaggtggcc gctgttgcga agatgcccgg
     1021 ctgcgcgtat cagttcggtc gcgtcctctg caatgtgttc gaggacgtcg atgtagagaa
     1081 tggcatcgaa ttggtcggtt gcgtcgagcg aggccatggt gccaatgcgg gaacgatgcc
     1141 ggttccctgc cggcagggca attcgagagg cgagtgcggc gtcgggttca aggcaggtcc
     1201 acgaatcgaa gcgctgcgtg gtgaacagtg ccgtgtttgc gccaataccg gcgcctactt
     1261 ccaggacgtc gccccgaatg gaggcggaga tgcggctgct ccaataggac ttccaattga
     1321 cggctttctc gaacaggtcg agttcggcgc cttcgtattg atactcggcg gcctgggcgg
     1381 cgtggctcac tttcggacct cggtggcggt ttgatcggta gctggcgggt ggaccgtggc
     1441 ggaatgtaag gccgcggcga ggagttcgag cgaggagcgc tcgccggcaa cggagacgcc
     1501 aagaatgaag tgccgtgcgt cgcgcggcaa cagaaatccg ggtgtggcgc ggcggtgggc
     1561 gatcaggaag acaaagaatg ctgtcagcag caggctttgc acggtgatgg cggccagggt
     1621 gatgaggcca tagatgacgg cgtcgggcag cgcgatcccg gccgaccatg cagcggttgc
     1681 cactgtggca agcgctgtga cagcgacacc cagcacggcg gaagccgtaa gcaaccgcgc
     1741 gctcacgtga tcgctgaaga ccgagatggc gctcagaccg tgcaccagga ggccggtgaa
     1801 gttcatctgt gaattgcccg cgagccgatt gccacgcgcc agaggcagaa gacgcctggg
     1861 gagtctcgcg cgaagtactg ccgcggcata gtgattccac aggtcgctga cggccatcag
     1921 ccgggcaagc gccgatggcg gcagcacgct gaaattcccg acgcggacct caatgccggt
     1981 cagggcgcga tggaggagcc ggtagagctg gtagaagaac tgaaagacaa aggactccat
     2041 ccgcttggcc cgggcggcaa agatggcatc gcggcggccg gagcgctcgt attccgcgag
     2101 aagggccagg atgtcggcgg cccggtcttc accgtcgccg tccataacga ccacggtcgc
     2161 cccgggacga gtttcgttga tgtgatacag gccgagcgct atggcgcgct ggtggccgag
     2221 gttggctcgg agatggagga cctcgatggg cacgagagac gcgggggcgg tctggggcca
     2281 gttcgccggc atcggcaggg tggaggcgtc gtcgaccacc agaaccgaag tgcaccaccc
     2341 tagccctgcc gcgaccgtat ccaattgatt cagaaggagt tgtagcggct cccagtcatt
     2401 gaaattgggg atcaggatga cgagtgagtc gctgtgagag gccatagtcc ccgcaacgag
     2461 tttgatcggc cggtaaagct agaggcttta ggagtttggc gggatgctac gatcctgaca
     2521 tgctgctcag gttggttctc gctcttgcct tttgcgcgtc gacgcaggct gagatccgca
     2581 agctaacgat tctgcatacg aacgatctcc atgccagggt gattccggac gacgcgggca
     2641 gaggcgggtt cgcctacgtt acaaacgtat tgcggcaaga gcgtaagggc tgtggtcact
     2701 gcattcacct gagcgcgggc gatctggtgc aggggatgcc ggtttcgtcc atattccggg
     2761 gcataccggt gtacgaaatg gccaaccgga tgggaatcga cgcccttacg ttaggcaacc
     2821 acgagttcga ctacggctgg cagcagattg agcggttccg ccggacggcc aagttcccac
     2881 tgctgtcggc aaacgcgttg gacgcggacg ggaaatccat cggcgacaaa ccgtacacgg
     2941 tgctcaaggt gaatggcctg cgtgtggggg tgattgggtt gttgatgggc aatctggtgg
     3001 agggttacct gacagcgaag acagcgggcc cggtgaagat agcaccggtg gtgggcgcgg
     3061 ctcgggcggc ggcaaatgac ctagctgggc gtgcggacgt catcatcgcg ttaggccacg
     3121 ttttgccggc tgaaggctcg gcaatcttga aagaagtgcc ggaggtggcg gccgtggtcg
     3181 aagggcatag ccacagagga cttgaagaga tggagcagtt gggcgggcgc gttcgtgtgg
     3241 gcctgaaggg atacggggtg gaggtcgggc ggctcgatct ggaggtggac actgcgacgc
     3301 accaggctgt ttcggcgcac tggaagcgga ttcccgtgga gtcaaggaaa gtgccgccgg
     3361 cggaggatgt cgccaaggtg gtggcgaagt gggaacgaaa agtggcgcag gttgtcgaca
     3421 agccggtggg cgaggcgcgc cgcgaatttc tgcagccgga cctcaaggtg ctgatggagc
     3481 aggcgatgtg cgaggagatg ggcgcggact tcagccatat gaaccggggc ggtgtgcggg
     3541 acaagctccc taaaggcacg atccgcgagc gcgagttgtg gaacatcatg ccgttcgaca
     3601 acacgatgat gctcgcccgc gtgaaggggc gtctgctgtc cgacgcgctg cgggcagggc
     3661 ggacggtgga gccgcagcgg gaatatgtag tggcgctggc cgactacagt gtggagaatg
     3721 aggcgcagcg gaaagagtta gggattgagg gatggacctt cgagcgcacc gacaagcagt
     3781 tgcggcaact gctgatcgac tgggtgaaga agaagaaagt gctggagtaa ggcacgggct
     3841 ggtatactaa tagagatcgg gccgtggcgc agcctggcta gcgcgcttgc ttggggtgca
     3901 agaggtcccg ggttcaaatc ccggcggccc gaccaaa
//
LOCUS       DASUGB010000285         8832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1420648, whole genome shotgun sequence.
ACCESSION   DASUGB010000285 DASUGB010000000
VERSION     DASUGB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420648"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="VFV87_05460"
     CDS             <1..2302
                     /locus_tag="VFV87_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103234.1"
                     /translation="ATLETEIDDLLASSSLRYSDSANQLVTGNFRLSQGADPKEVLDT
                     YYNNVRRRQPNNPDVYLAIGALALEKGDYLMAGDAFQQAAKLDALDPAAHFGVAQSFA
                     PSDSEKAEAAINLALEKNPNHVPSLLLIADEQIDGEQYDEADQTLAKIAAINPHHPRA
                     LAYKAVLAHLRNQPEKEKQHRAAALRTWPTNPEVDYLIGKKLSQKYRFAEGEQYQRQA
                     LQFDPKYLPARSQLASDLLRLGREEEGWKLAGDVYDADGYNVNAHNLVTLQENIAKFA
                     TLEEDGLTLRMEPREAEIYGRRVLELLKRAKQTLAAKYDVQLQGPIVVEMFPQQPDFA
                     IRTFGMPGGAGFLGVCFGTVITATSPAAQGASPSCWESTLWHEFCHVVTLNKTNNKMP
                     RWLSEGISVYEERQADKTWGQRITPQYRAMILGDDLVPVSQLSSAFLSPKTPLHLQFA
                     YFESSLVVEYLIEKYGLPTLQRVLVDLSVGMPINESLGRYAGSIEALDKEFADYARQH
                     AEAMAPKADWSEPELPRRASAELIAAWLKDHPNNYAALLRLAQQQVNDEQWGEAIQTA
                     EKLRDLYPSDQSAAGPYGILAAVYKELGKNKEERAVLERWAELSDDDTAMFSRLIELT
                     QKEGDFTSTKRYALRWLGVNPLIPAPHRAAAAAAEALGEPALAAPCYQALLRLEPIDT
                     AELHLKLATALRKTGDLPAAKRQALLALEETPRYRAAHQLLLAIAADPRSTRPATTPK
                     PSEKPPAQTKKPETKPPAIPPPATKP"
     gene            2302..3159
                     /locus_tag="VFV87_05465"
     CDS             2302..3159
                     /locus_tag="VFV87_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5103235.1"
                     /translation="MKSWTRVLSMAFVLAIVSAVAMAQFGFDPFREGRRGRGRGRSWS
                     RETRGEDQDRGGVPEWKNDERFKEDVFTFTRVRYSSWGRVKWDTDYPDSDFNFSYRLH
                     ELTALETDPYGKIVELTDDALFDYPWLYLIEPGEMELNEAEVLALRKHLQNGGFMMVD
                     DFWGTDELENLLAEMKRVFPDREPIELPLSHDIFHCVYDLKEKPQIPSIHAWWGGRTS
                     ERGSDSETPHYMGIFDDKGRMMVIICHNTDVGDGWEREGLDNEYFKQFSEKWSYPLGI
                     NILVYAMTH"
     gene            3276..4286
                     /locus_tag="VFV87_05470"
     CDS             3276..4286
                     /locus_tag="VFV87_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5103236.1"
                     /translation="MELDAYEMEQQVVEQIRGSKGRIDAELRKVIVGQAEVVEQLLIS
                     LFAGGHCLITGAPGLAKTLLVRSIAQIFHLKFQRIQFTPDLMPADITGTEILEQEVDG
                     HRRMQFVKGPIFGNVILADEINRTPPKTQAALLEAMQEHQVTVAGTRYALEEPFFVLA
                     TQNPIEMEGTYPLPEAQLDRFMFNVLIDYLPEEDEVAVVKQTTSKRPAPIEPIFTGED
                     VRRFHEVVQRVPIAEDVIRYAVRLAAASRPGQTGSPGFVNDWVSWGAGTRAAQYVVLG
                     AKARALLAGRNHATVQDIQALVHPTLRHRILVGYRAEAEGVTVENVISRLLETVPEPQ
                     AK"
     gene            4325..5323
                     /locus_tag="VFV87_05475"
     CDS             4325..5323
                     /locus_tag="VFV87_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5103237.1"
                     /translation="MTRSGPGRQAGSAAASFIDAAALMRIKNLQLRAKVVVEGFLSGL
                     HRSPYHGFSVEFTEYREYSPGDDLRYLDWKLFARSDRYYLKRFEDETNLRCYLLVDLS
                     KSMGFGSLAYDKAQYAKTAAATIAYFLSLQRDAVGLVTFDAAIKDYLPARFRPGHLHR
                     LMLCLEQAPAGTGTDLAAPLEQIARTARKRGLVVLLSDLLAPLGELETKLGYLRSRGH
                     EVVVIRILDPDEIDFPFDEAAMFHDLESGRELYVDPQLVRERYQQRFGEHRAGLTTIC
                     GKLGIDLYELSTSQPLEMALFDFVNARMRRGRHVSRRQGLRGQSPVRQTSAAGGAR"
     gene            5323..7473
                     /locus_tag="VFV87_05480"
     CDS             5323..7473
                     /locus_tag="VFV87_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5103238.1"
                     /translation="MGILAPLYLAGLAALSLPLILHLVRRTPKGRKDFSSLMFLSPTP
                     PRLTRRSRLDQILLLLLRLAALALLAFAFARPFLRQEATLTMNSLPSRRVAILLDTSA
                     SMRQTGVWQRAIQQVEQELNDLGPQDEVALYTFGDRLRTHVAFDRTTSQPASVGDALP
                     KVELARQALKSLEPTWSATDLGAALVAVASELDAASDVQQSAAEPQLVVISDFPKGAR
                     IEALQSYEWPKNVRLIAREVRPKQPTNAYVHLLRSETEEESEELRIRVVNSPGSSGDQ
                     FFVHWQEEAGPKDSLAYASGSSDASETAVYVPPGQSRVIKLPRPADRLQADRLVLTGD
                     DHDFDNVHFVVPPLKQHVQIVYAGSDAADDALGMQYYLRLATSGDPLRQVEFLPLAGD
                     DAKPLRATPPPKLAVVTREVSAALAAELKSFAERGGMVVLAPPDQAAAVAIPQLVDDV
                     ELLPAPAESEAGGEDYLLLGEIDFTHPLFAPFAGPRYSDFTKIHFWMHRGLKLKEGAP
                     SHVVAKYDSGAPAIVERALGDGRVLVLASGWNPDDSQLALSTKFVPLVGGILDRACGI
                     AAPKIGVTVDDPVPLDAGRRFATVITRPDGKDVEVAASSASFTDADQPGVYRAAAGSE
                     VSRFAVNLAASESNTAPLEMDQLKQLGVRLSESMTRAERLSQMRQERDTELESRQKLW
                     RWIVLGTLGLLIFETFWAGRTARQIAQAESTALG"
     gene            7526..>8832
                     /locus_tag="VFV87_05485"
     CDS             7526..>8832
                     /locus_tag="VFV87_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103239.1"
                     /translation="MHQQRLWFELDRVALRFRMLRFWLALAAAWLIAALLGLTLFGLR
                     ASFAAPAGVAVTLVCLVAAGLAVAGIWLAATSARSHEWVARRIEAAFPELRTCLLAAV
                     EQRPDLPFGRFGYLQSSVIHQALRHADRHAWTEVVPMRRIALAAGANLLTFVMFVGVL
                     ALVAMTAGPAAERAAALASAARDSLTAKAFSVTVEPGNTEVERGTSLLVLARITGSMP
                     AEATLVFVAESGEESRLPMSPSLDDPVFGGRIPLVDAPLDYRVELGGQTSPTYHITVF
                     EYPRLEKADARLVFPAYTKLPEKLVQDVRTVSVVEGTDLTLMCYLNKPVATAALIEDQ
                     QPPILLTAATPPAPSTEEVDDAKAGAKAKPSRRTQYFYTAQIKCEQSRKLKLELTDEA
                     GRKNVKLVTFTINVLPNQAASFKPTFPARDLEVSPLEELDVKAT"
BASE COUNT         1608 a         2829 c         2885 g         1510 t
ORIGIN      
        1 cgcaacactg gaaacggaga tcgatgacct gcttgcgtcg tcgtcgttgc gctacagcga
       61 ttccgccaac cagctcgtca ccggcaattt tcgcttgagc cagggcgcgg atcccaagga
      121 ggtcctcgac acgtattaca acaacgtccg ccgccggcag ccgaacaatc ctgacgttta
      181 tctcgcgatc ggggcgctgg cgctggaaaa aggcgactac ttgatggcag gcgacgcttt
      241 tcagcaggcg gccaagctcg acgcgctcga cccggcggcg cacttcggcg tggctcagtc
      301 gttcgccccg agcgattcgg agaaagcgga agccgcaatc aatctggctc tcgaaaagaa
      361 cccgaatcac gtgcccagcc tgctcctgat cgccgatgag cagatcgacg gcgagcagta
      421 cgacgaagcc gaccagacgc tggccaaaat tgccgccatc aatccgcacc atccgcgcgc
      481 cttggcctat aaggcggtgc tcgcccactt gcgcaatcag cccgagaaag agaagcagca
      541 ccgcgccgcc gccctgcgga cctggccgac gaatccggag gtcgattacc tcattggcaa
      601 aaagctctcg caaaagtatc gcttcgcgga aggcgagcag tatcagcggc aggcgctcca
      661 gttcgatccc aagtatttgc cggccaggtc gcagttggca agcgacctct tgcggctggg
      721 gcgcgaagag gagggctgga agctggccgg cgacgtctat gacgccgacg gctacaacgt
      781 caacgcgcat aacctggtca cgctgcagga gaacattgca aaatttgcga ccctcgaaga
      841 ggacggcctg accctgcgga tggagccccg cgaagccgag atttacggcc ggcgcgtgct
      901 ggagctgctc aagcgggcga agcagacgct ggcagccaag tacgacgtgc aattgcaggg
      961 gccgatcgtg gtcgaaatgt tcccccagca gccggacttt gccatccgca cattcggcat
     1021 gcccggcggc gcgggctttt tgggcgtctg cttcggcacg gtgatcacgg cgaccagccc
     1081 ggccgcgcag ggggcgagcc cgtcgtgctg ggaatcgacg ctgtggcacg agttctgcca
     1141 cgtggtgacg ctcaacaaga ccaacaacaa gatgccgcgg tggctgagcg aagggatttc
     1201 ggtctatgaa gagcggcaag ccgacaagac ctgggggcag cggatcacgc cgcagtaccg
     1261 ggcgatgatt ctcggcgatg acctggtgcc ggtcagccag ctcagcagcg cgtttctcag
     1321 tcctaagacg ccgctgcatc tgcagttcgc gtatttcgag tcgtcgctcg tcgtcgaata
     1381 cctgatcgag aagtacggcc tgccgacgct ccagcgggtg ctcgtcgatc tgagcgtggg
     1441 gatgccgatc aatgaatcgc tcggccgcta tgccggctcg atcgaggccc tcgacaagga
     1501 gtttgccgac tacgcccgcc agcacgccga ggcgatggcc cccaaagccg attggagcga
     1561 gcccgaactg ccgcggcgag cgagtgccga actgatcgcg gcctggctca aggaccatcc
     1621 caataattat gcagccctgc tgcggctggc gcagcagcag gtgaatgacg agcagtgggg
     1681 ggaggcgatc cagacggctg agaagctgcg tgatctgtac ccgagcgacc agagcgcggc
     1741 ggggccttat ggcatcctgg ctgccgttta taaggagctg ggaaagaaca aggaggagcg
     1801 ggcggtgtta gagcgttggg cggaattgtc tgacgacgac acggcgatgt tctcgcggct
     1861 gatcgagcta acacaaaaag agggggattt tacttccaca aaaaggtatg cgcttcgctg
     1921 gctgggggtg aatccgctca tcccggctcc gcaccgagca gcggcagcgg cggctgaggc
     1981 gctcggcgag ccggccctgg cggcaccgtg ttaccaggcg ctgttgcgtc tggagccgat
     2041 cgacactgcg gagctgcacc tgaagctggc aacggcgctg cggaaaacag gcgacctgcc
     2101 cgcagccaag cggcaagcgc tcctggcctt ggaggagacg ccccggtacc gggcggccca
     2161 tcagttgctg ctcgcgatcg ccgccgatcc gagatccact cggcccgcaa caacacccaa
     2221 gccttccgaa aagccgcccg ctcaaaccaa gaagcccgaa accaagccgc ccgccattcc
     2281 ccctcccgct accaagccat gatgaaatcc tggacgcgcg tgctgtcgat ggcatttgtg
     2341 ctggcgattg tgtcggcagt ggccatggcg cagtttggct tcgatccctt ccgcgaaggc
     2401 cggcgtggtc gcggccgcgg ccgctcctgg tcgcgggaga cccgcggcga agaccaggat
     2461 cgtggcggcg tgcccgaatg gaagaacgac gaacgcttca aggaagacgt gttcacgttc
     2521 acccgcgtgc ggtactcgtc gtggggccgc gtcaaatggg acaccgacta tcccgatagc
     2581 gatttcaatt tctcgtatcg gttgcacgag ctgaccgcac tcgaaaccga tccctacggc
     2641 aagatcgtcg agctgaccga cgacgcgctg ttcgactatc cctggctcta cctgatcgag
     2701 cccggtgaga tggagctgaa cgaagccgag gtgctcgcct tgcgcaagca cttgcagaac
     2761 ggcggcttca tgatggtcga cgatttctgg ggcaccgacg agctggagaa cctgctcgcc
     2821 gagatgaaac gcgtttttcc cgatcgcgag ccgatcgagc tgccgctctc gcacgacatc
     2881 ttccactgcg tctacgactt gaaagagaag ccgcagattc ccagcatcca cgcctggtgg
     2941 ggcgggcgca cgtcggagcg cggcagcgat tcggaaacgc cacactacat gggaatcttc
     3001 gacgacaagg gccggatgat ggtcatcatt tgccacaaca ccgacgtcgg cgacggctgg
     3061 gagcgcgaag ggctggacaa cgagtacttc aagcagtttt ccgaaaagtg gtcgtatccg
     3121 ctgggaatca atattctggt gtatgcgatg acgcattaag agtgagttgt gagttgtgag
     3181 tggtgagtgg tgagtggtga gtaagggaat acgactcacc cacttaccca ctcaccactc
     3241 acctgcccca ccgagaggac tgattgcccg tgtcaatgga actagatgcc tatgaaatgg
     3301 aacagcaggt ggtggagcag attcgcggca gcaaggggcg catcgacgcg gagctccgca
     3361 aggtcatcgt ggggcaggcc gaggtggtcg agcaattgct catcagcctg ttcgcgggcg
     3421 gccattgcct cattaccggc gcgccgggcc tggccaagac gctcctggtc cgctcgatcg
     3481 cgcaaatctt ccacctgaag ttccagcgga ttcagttcac gcccgacctg atgcccgccg
     3541 acatcaccgg caccgaaatc ctggagcagg aggtcgatgg gcaccgccgg atgcagttcg
     3601 tcaaagggcc gatcttcggc aatgtgatcc tggccgacga aatcaaccgc acgccgccca
     3661 aaacgcaggc cgcgctgctg gaggcgatgc aggagcacca ggtcacggtc gccggcacgc
     3721 gctacgccct cgaagagccg tttttcgtcc tggccacgca gaacccgatc gagatggagg
     3781 gcacgtatcc gttgccggag gctcagctcg accgcttcat gttcaacgtg ctgatcgact
     3841 acctgccgga agaggacgag gtggcggtgg tcaagcagac cacgtccaag cggccggctc
     3901 cgatcgagcc gatcttcacc ggcgaggatg tacgccgctt ccacgaagtc gtacaacgcg
     3961 tcccgattgc cgaggacgtg attcgctacg ccgtccgtct cgccgctgcc agccgtccgg
     4021 gccagaccgg ctcgcccggc ttcgtgaacg actgggtgag ctggggcgcc ggcacgcggg
     4081 ccgcgcagta cgtagtcctc ggcgcgaagg cccgcgccct cctggccggc cgcaatcacg
     4141 cgaccgtcca agatatccag gccctcgtcc atcctacgct ccgccaccgc atcctcgtcg
     4201 gctaccgcgc cgaagcggag ggcgtgacgg ttgaaaacgt gatcagtcgg ctgctggaaa
     4261 cggtgcccga gccacaagcc aaataactcc gcattttgcc atggcgcggt ccaagctcag
     4321 ttcaatgacg cgctccggtc ccggccgcca ggcaggatca gcggctgctt cgttcatcga
     4381 cgcggctgcc ttgatgcgga tcaagaacct gcaattgcgg gcgaaggtcg tcgttgaggg
     4441 gtttctgagc ggcctccatc gcagcccgta tcacgggttt tcggtcgagt tcaccgagta
     4501 ccgcgagtat tcgccgggcg acgacctgcg ttacctggat tggaagctct ttgcccgctc
     4561 cgaccgctac tatctgaagc ggtttgagga tgagaccaac ctccgctgct acttgctcgt
     4621 cgatctctcc aagtcgatgg ggtttggctc gctggcgtac gacaaggccc agtacgccaa
     4681 gacggccgcg gcgacgatcg cctatttcct ttcgctgcag cgcgacgcgg tgggcctggt
     4741 gacgttcgac gccgcgatca aggactattt gcccgcccgc tttcgccccg gacacttgca
     4801 ccgcttgatg ctctgcctgg agcaggcgcc cgccggcacg ggcaccgacc tggccgctcc
     4861 gctggagcaa atcgccagga cggcccgcaa gcgcggcctg gtcgtgctgc tgtccgatct
     4921 gctcgcgccg ctgggcgagt tggagacgaa acttggctac ctgcggtcgc gcggccacga
     4981 ggtcgtcgtc atccgcattc tcgatccgga cgagatcgat tttccgtttg acgaagcggc
     5041 catgtttcac gatctggaga gcggccgcga gctgtacgtc gatccgcagc tcgtccgcga
     5101 gcgctaccag cagcgattcg gcgagcaccg cgccggcctg accacgatct gcggcaagct
     5161 cggcattgac ctctatgagc tgtccaccag ccagccgctg gagatggccc tgttcgattt
     5221 cgtcaacgcg cggatgcgcc gcgggaggca cgtcagccgg cgccaaggcc tccgaggaca
     5281 aagcccggtg cggcagacct cggccgcagg aggcgctcgc tgatgggaat cctcgcgccg
     5341 ctctacctgg ccggcctggc ggctctgtcg ctgccgctga tcctgcacct ggtccgccgc
     5401 acgcccaagg ggcgcaagga cttcagctcg ctaatgttcc tgtcgcccac gccgccgcga
     5461 ctcacgcgcc gcagccggct cgatcagatt ttgctgctcc tcttgcggct ggcggcgctg
     5521 gcgctgttgg cgttcgcgtt tgcccggccg tttttgcggc aggaggcgac gctgacgatg
     5581 aacagcctcc ccagccgccg cgtggcgatt ttgctcgaca ccagcgccag catgcggcag
     5641 accggcgtct ggcagcgggc gatccagcaa gttgagcagg agctgaatga cctcggcccg
     5701 caggacgaag tcgcgctcta cacgttcggc gatcggctga ggacgcacgt ggccttcgat
     5761 cgcacgacct cgcagcccgc gagtgtcggc gatgcgctcc ccaaagtcga gctcgcgagg
     5821 caagcgctga aatcgctgga gcccacgtgg agcgccacgg acctcggagc ggcgctggtc
     5881 gcggtggcca gcgagctcga cgccgccagc gacgtgcagc aatccgccgc ggagccgcag
     5941 ctcgttgtga tcagcgattt tccgaagggg gcgcggatcg aggccctgca atcgtacgag
     6001 tggcccaaga acgtgcggct gattgcccgc gaggtccgcc ccaagcagcc gacgaacgcc
     6061 tacgtccatt tactgaggag tgagaccgag gaggagagcg aggaactgcg gattcgcgtc
     6121 gtcaacagcc cgggctcgtc gggcgatcag tttttcgttc actggcagga ggaggccggg
     6181 ccgaaggact ccctcgctta cgcttcgggt tccagtgacg ccagcgaaac ggccgtctat
     6241 gttccgcccg ggcaaagccg ggtgatcaag ctgccgcggc cggccgaccg gctgcaggcg
     6301 gaccggctcg tgctgacggg cgatgaccac gatttcgaca acgtgcactt cgtcgtccca
     6361 ccactcaagc agcacgtgca gatcgtttac gccggcagcg atgcggccga cgacgccctg
     6421 ggcatgcagt attacctgcg gctggcgacc agcggcgatc cgctcagaca ggtcgagttc
     6481 cttccacttg ctggcgacga tgcaaagccc ttgcgagcga cgccgccgcc caagctggcg
     6541 gttgtcaccc gcgaggtttc cgccgcgctg gccgctgagc tgaagtcgtt tgccgagcgg
     6601 ggcggaatgg ttgttctcgc cccgccggat caagccgccg cagtggcgat tccgcagctc
     6661 gttgacgacg tggagctgct gccggcgcct gccgagagcg aagccggcgg cgaggattat
     6721 ctgctattgg gcgagatcga cttcacgcat ccgctctttg ccccgtttgc cgggccgcgc
     6781 tacagcgatt tcacgaagat tcatttctgg atgcaccgcg gcctcaaact gaaggagggc
     6841 gcgccgtcgc acgtcgtggc caaatacgac agcggcgctc cggcaatcgt cgagcgggcc
     6901 ttgggcgacg gccgcgtgct ggtgctggcc agcggctgga atccggacga cagccagctc
     6961 gccctctcga cgaaattcgt gccgctcgtc ggcggaattc tggaccgggc ctgcgggatc
     7021 gcggcgccga agatcggcgt caccgtggac gatccggttc cgctggatgc cggccgacgc
     7081 ttcgcgacgg tcatcacccg gcccgacggc aaggatgtcg aagttgcggc cagcagcgcg
     7141 agtttcacgg acgccgatca gccgggcgtg taccgcgcgg ccgccggttc ggaagtgtcg
     7201 cgattcgccg tgaatctggc cgcgagcgag agcaacaccg cgccgctgga gatggaccag
     7261 ctcaagcaat tgggcgtccg tctctcagaa tcaatgacgc gggccgagcg cttgagccag
     7321 atgcgccaag agcgcgatac ggaactggaa agccgccaga aactctggcg gtggatcgtt
     7381 ctgggaacgc tgggcctgct cattttcgag acgttctggg ccggccgcac cgcgcggcag
     7441 atcgcgcagg cggaatcgac ggccctcggt taggatgaaa cagccagaat gaagcagtgg
     7501 cggcaggccg cccgaggaga cagccatgca ccagcagcga ctgtggttcg agctcgaccg
     7561 ggtggccttg cggttccgga tgctccggtt ctggctggct ttggccgcgg cctggctgat
     7621 cgccgctctt ctaggtttga cattattcgg gctgcgggcg agcttcgcgg ctcccgcggg
     7681 cgttgccgtg acgctggttt gcctcgttgc cgcgggcctg gcggtcgccg gcatctggct
     7741 ggccgcgacc agcgcccgca gccacgagtg ggtcgcgcgg cgcatcgagg ctgcgtttcc
     7801 cgaactgcgg acgtgcctgc tggcggccgt cgagcagcgt cccgatctgc cgtttggccg
     7861 gttcggctat ttgcaatcga gcgtgattca ccaggcgctg cgccacgcgg atcgccatgc
     7921 ctggaccgag gtcgtgccga tgcggcggat cgccctggct gccggggcga atctgctgac
     7981 gttcgtgatg tttgtcggcg tgctcgccct ggtggcgatg acggctggcc ctgccgccga
     8041 gagggccgcc gcactggcga gcgccgcccg cgattcgctc acggccaagg cgttcagcgt
     8101 caccgtcgaa ccgggcaaca ccgaggtcga acgcggcacg agcctgctcg tgctggcccg
     8161 gatcaccggc tcgatgccgg ccgaggccac gctcgtgttc gttgcggaaa gcggcgagga
     8221 atctcggctg ccgatgtcgc cgagcctcga cgatccggtc tttggcgggc ggattccgct
     8281 ggtcgatgcg ccgctcgact accgcgtcga attgggcggg cagacttcgc ccacgtatca
     8341 catcaccgtg ttcgagtacc cgcgcctgga aaaggccgac gcccgcctcg tcttcccggc
     8401 ttatacgaag ctgcccgaga agctggtgca ggacgtccgc accgtcagcg tcgtggaagg
     8461 gaccgacctg acgctgatgt gctatctgaa caagccggtc gcgaccgccg cgctgatcga
     8521 agatcagcag ccgccgattc tgctgaccgc ggccacgccg cccgcgcctt ccacggaaga
     8581 agtggatgat gcgaaggcgg gcgcgaaggc gaagccttcg cgccgcacgc agtatttcta
     8641 tacggctcag atcaagtgcg agcagagccg caagctcaag ctcgaattga ccgacgaagc
     8701 cggccgcaag aatgtcaagc tggtcacgtt cacaatcaac gtgcttccca atcaggcagc
     8761 gagcttcaag ccgacgtttc cggcccgcga tctggaggtt tcgccgctgg aagaactgga
     8821 tgtgaaggcg ac
//
LOCUS       DASUGB010000286         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1143402, whole genome shotgun sequence.
ACCESSION   DASUGB010000286 DASUGB010000000
VERSION     DASUGB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143402"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VFV87_05490"
     CDS             <1..132
                     /locus_tag="VFV87_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX5103240.1"
                     /translation="RTVSWLHNFRRLRIRWERRDDIHEAFMTLAEGLICFSIICGLC"
     gene            126..917
                     /locus_tag="VFV87_05495"
     CDS             126..917
                     /locus_tag="VFV87_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5103241.1"
                     /translation="MLNPLNWSVSLFGRAPLPPLAESDESLREVCESDGVRILEASAG
                     SDSVVQFLVSTRPSAAPAQILRCLKGRWQYLIRKTHPAAFKRNYRIESVGSSSTDVIE
                     KYIAGQLPRQGMADSRAQAMLARFQIDNPAVDMKRVRYTSYGQFTYNLHLVVVRESEL
                     LMDEPSLSANRDMILKVSARKELLLRRGGILSDHLHLALGRGIEQSSEEIALSHLNNL
                     AFANGMTPCFRFGYYVGTFGAYDLGAVRVGLAESHGSAGTSPAGM"
     gene            1002..2402
                     /locus_tag="VFV87_05500"
     CDS             1002..2402
                     /locus_tag="VFV87_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5103242.1"
                     /translation="MRLERLFRSILEPAGTSGASGIELSPTSLLPLVIGLAIVAVLAI
                     LGKVPVRYNVRNLTVRWRTTLMTSLAFTLVIGLNTVMLAFVNGMYKLTQGSGQPGNVM
                     VLSEGATDEAFSNLGFSDIGDIELQPGVLRDENDQPLVSRETYVVVNQPVRIRAAGRP
                     RGRFTQVRGVDDAAMSAFVHGLDLYEGGQWITREGVRAAGEGQEAIQAVIGEGIAREL
                     GRDRPPEELAKAKNPERLEVGETFPLGGRTWLVVGVIKYSGSTFDSEIWAKRDIVGKM
                     FGKPSYTSLVLRTADAAEATKLEYFFNNDFAKASVQAYRETEYFAALAETTKQFLFSI
                     IFVTLVMAVGGIFGVMNTMFAAVAQRAKDIGVLRILGFSRLDVQASFLLESLVIALAG
                     GALGCALGCFAHGWQSASILSRNEPGSSKFVVLELIVSGDIIAAGLAVALVIGLIGGI
                     IPSIRAMLVRPLESLR"
     gene            2521..>2770
                     /locus_tag="VFV87_05505"
     CDS             2521..>2770
                     /locus_tag="VFV87_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011124108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5103243.1"
                     /translation="MDAPLPPLESHLARFGLASFRTGQREVISTVLAGQDCLCLMPTG
                     GGKSLCYQLPGLASADLTVVVSPLIALMRDQWLRLSGAG"
BASE COUNT          468 a          835 c          917 g          550 t
ORIGIN      
        1 cgcacggtgt cttggctgca taatttccgt cgcctgcgga tacgatggga acgccgtgac
       61 gacattcacg aggccttcat gaccctcgcc gagggcctca tctgcttcag catcatctgc
      121 gggttgtgtt aaaccctctt aattggtcgg tctcgctgtt cggtcgcgcc cctcttccgc
      181 cattggctga atcggatgaa tccttgcgcg aggtgtgcga aagcgatgga gtgagaattc
      241 tcgaagcctc ggccgggtcg gactctgtcg ttcaattcct ggttagcacg cggccctcgg
      301 ccgcgcccgc gcaaatcttg cgatgcttga aaggccgctg gcagtacttg attcgtaaga
      361 cgcacccggc cgctttcaag cggaactatc gaatcgaaag tgtcggttcg tcgagcacag
      421 atgtgatcga gaagtacatc gccggccagt tgccccggca gggaatggcc gattcgcgag
      481 cacaagcgat gctcgcgagg tttcaaatcg acaatcccgc cgtggacatg aagcgagtcc
      541 gctacacgtc gtacggccag ttcacctata acttgcacct ggtcgtcgtg cgcgagtcgg
      601 aactcttgat ggatgaacct tctttatcag ccaatcgcga catgatcttg aaagtctcgg
      661 cgaggaagga actgctgctt cgccgaggag gaattctcag cgaccatttg catcttgcgt
      721 tgggccgcgg gattgagcaa tcgtccgagg aaatcgcctt gtcgcatctg aacaacctgg
      781 catttgccaa cggtatgact ccgtgctttc ggtttgggta ttacgttggg acgtttggcg
      841 cctatgacct cggcgcggtg cgcgtcggtt tagcggaaag tcatggttcc gccgggacaa
      901 gcccggcggg gatgtgattt ggaggacgag gcgtagtagc ggtcagtcat gattccgccg
      961 gggcaagccc ggcggggaaa tgcgaagccc aaggttgctt aatgagactt gagcgcctat
     1021 ttcgatcgat cctcgaaccc gcgggaacgt ccggcgcgtc cggaatcgag ctgtcgccca
     1081 cgagcttgct gccgctggtg attgggctgg cgattgtcgc ggtgctggcg atcctgggca
     1141 aagtgccggt gcggtacaac gtccgtaatc tgaccgtccg ctggcggacg acgctgatga
     1201 cctccttggc gttcacgctg gtcatcggcc tcaacaccgt gatgctggca ttcgtcaacg
     1261 gcatgtacaa gctcacacaa ggcagcggcc agccggggaa cgtgatggtc ctctcggagg
     1321 gggcgaccga cgaggccttc agcaacctgg gcttttcgga catcggcgat atcgagctgc
     1381 agccgggcgt gctgcgcgac gagaatgacc agccgctcgt cagccgcgag acgtacgtcg
     1441 tcgtcaacca gccggtgcgg attcgcgcgg ctggccgtcc gcgggggcgg ttcacgcagg
     1501 ttcgcggcgt ggacgatgcg gcgatgtcgg ccttcgtcca cggactcgat ctgtacgagg
     1561 gggggcagtg gatcacgcgc gaaggggtgc gtgccgcggg cgaaggtcag gaagcaatcc
     1621 aggcggtcat tggtgaggga atcgcccgcg aactggggcg cgaccggccg ccggaagagc
     1681 tggcgaaggc caagaacccc gagcgactgg aggtggggga gacgtttccg ctgggcgggc
     1741 ggacttggct cgttgtcggc gtgatcaagt attccggttc gacgttcgat tccgaaatct
     1801 gggccaagcg cgacatcgtc ggcaagatgt tcggcaagcc gagctatacc tcgctcgtgc
     1861 tgcggacggc cgacgccgcc gaagccacga agctggaata cttcttcaac aacgacttcg
     1921 ccaaggcttc ggtgcaggcc tatcgcgaga cggagtattt tgcggccctg gcggaaacga
     1981 ccaagcagtt cctgttctcg atcatcttcg taacgctcgt gatggcggtg ggcggcatct
     2041 tcggcgtgat gaacaccatg ttcgccgccg tcgcgcagcg ggcgaaggac atcggcgtgt
     2101 tgcggatcct cggcttctcg cggctggacg tacaggcgtc gtttttgctc gaatcgctgg
     2161 tgatcgccct ggccggcgga gcgctgggat gtgctctggg ctgcttcgct cacggctggc
     2221 aatcggccag catcctcagc cgcaacgagc ccggcagcag caagttcgtg gtgctggagc
     2281 tgatcgtgag cggcgacatc atcgcggccg gcctggcggt ggcgctcgtc atcggcctga
     2341 tcggcggcat catcccatcg attcgggcga tgctcgtgcg tccgctggaa tcactgcggt
     2401 agccgtaggg tgggacaagg ccgccgcggc cgccggccca ccattccttg gatgctgcgt
     2461 cgcggtgggc ctgcgcgtcg ctacgcgacg ctggtcccac cctacattac aatcgccgcc
     2521 atggacgcgc ccctcccacc cctcgagtcg cacctggccc gcttcggcct ggcgagcttt
     2581 cgcacgggcc agcgggaggt gatttccacg gtgctggccg gccaggattg cctgtgcctc
     2641 atgccgaccg gcggcggcaa gagcctctgc taccagctcc ccgggctcgc ctcggccgac
     2701 ctgaccgtcg tcgtcagccc gctgatcgcg ctgatgcgcg accagtggct gcgcctctcc
     2761 ggcgccggcc
//
LOCUS       DASUGB010000287         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1282320, whole genome shotgun sequence.
ACCESSION   DASUGB010000287 DASUGB010000000
VERSION     DASUGB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282320"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFV87_05510"
     CDS             complement(<1..894)
                     /locus_tag="VFV87_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX5103244.1"
                     /translation="MLAGSIAFLVIGFAILIYSGDCLVRGALAAAFKTNVSPLLVGIL
                     IVGFGTSLPEFLIGVQAALAGTPGLAHGNIIGSNIANIWLVLALPAVIFPISTVAPRM
                     RLTAFFMLLCTAAWIGITLYFGLNALTGMWFLIVLGGYAIVAWFSHPRDDTEDTPDEK
                     KIMATPAWRMVFLILIGLVGLPLGSKLLVDAGISLADEIGASKEIVGLTLLAVGSSLP
                     ELAAGVAAAVRKQSDVAMGNILGSNIFNILGVGGVVAFLAPQPTLAEAFTSYSNWAMG
                     LAAVLVTLVAFLHRRVGWLTGA"
     gene            1181..1636
                     /locus_tag="VFV87_05515"
     CDS             1181..1636
                     /locus_tag="VFV87_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5103245.1"
                     /translation="MRKRTLRDRAFQSFWRMRRPATLGVRGVVTNERGEVLLVRHTYT
                     PGWHFPGGGVEKLETCALSLKRELQEEAGIEADGLQLVSVHANHAFFPNDHVLVFRVT
                     AWRQGKILQRGEIAEICFADPLNLPDGVTAGTKRRLDELFAGKPPSDLW"
     gene            1687..>2215
                     /locus_tag="VFV87_05520"
     CDS             1687..>2215
                     /locus_tag="VFV87_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103246.1"
                     /translation="MFADQCRPALEELMRGAWIAPLLLGFALAGCGGSTPAPAESSTA
                     ALEGDAALIASAQVISDQLGGCTKPDGAAVESKVVGLESGPIVLLACSQDKFTYTHRL
                     FAIRAGQKPELISLPDYDDRGMFATDQASLAELDAGTGVLTTFRKAAEHGRCGSEARY
                     QWNGERFDLQELHWQD"
BASE COUNT          433 a          681 c          735 g          366 t
ORIGIN      
        1 ggcgcctgtg agccagccga cgcggcggtg caggaaggcg accagcgtga cgaggaccgc
       61 cgcgaggccc atcgcccagt tgctgtagct ggtgaaggcc tcggcgagcg tgggctgcgg
      121 ggccaggaag gcgacgacac cgccgacgcc gaggatgttg aagatgttgg agccaaggat
      181 gttgcccatg gccacatcgc tctgcttgcg gacagcggcg gcgaccccgg cggcgagttc
      241 cggcagcgaa gagccgacgg caagcagcgt caggccgacg atttccttgc tggcgccgat
      301 ctcatcagcg agcgaaatac cggcatcgac aagcagtttc gagcccagcg gcaggccgac
      361 gagtccgatc aggatcagga agaccatccg ccaggccggc gtcgccatga tcttcttttc
      421 gtcgggggtg tcttcggtgt cgtcccgcgg gtgggagaac caggcgacga tggcatagcc
      481 gccgaggacg atcagaaacc acatccccgt cagcgcattg aggccgaagt agagggtgat
      541 gccgatccat gccgccgtgc acagcaacat gaaaaatgcg gtcagacgca tacgcggcgc
      601 cacggtcgag atcgggaaga tcacggccgg cagcgcaagc accagccaga tgttggcgat
      661 gttggagccg atgatattgc cgtgcgcgag gccgggcgtt ccggccagcg ccgcttgcac
      721 gccgatcagg aattccggca gcgacgttcc gaagccgacg atcaagatgc cgacaagaag
      781 cggcgagacg ttcgtcttga acgcggcagc gagcgcgcca cgcacgaggc agtcacccga
      841 atagatgaga atcgcaaagc cgatgacgag gaaagcgatc gatcccgcga gcatccccca
      901 cgtcctcccc gcacaatccc ccagtcattc ccccgagtgt cccggaggtt caagccttgg
      961 tccgacagct agtccagacg gcggtattcc agcaggttga gcacagcgaa aaccgccaca
     1021 acagccagga ttgcgtacac agcggccacg tttagctcct tggttccggt ctgacttgag
     1081 tcccctgtcc ggcccttcta tcccgcttct tccggggtgg ctatgccgcc attgcgacgc
     1141 agttggcagg ctattgcacg acctagcgga aggctttccg atgagaaaac gcacgcttcg
     1201 cgaccgggcc ttccagtcct tctggcggat gcgccggcca gcgaccctgg gcgtcagggg
     1261 cgttgtgacc aacgagcggg gagaagtgct gctggtacgt cacacctaca cgccggggtg
     1321 gcatttcccg ggcggcggag tggagaaact ggagacctgc gcgctgtcgc tgaagcggga
     1381 gctgcaggaa gaagccggaa tcgaagccga cgggttgcag ctggtctccg tccacgcgaa
     1441 ccacgccttt ttccccaacg accatgtgct ggtgttccgg gtcaccgcgt ggcggcaggg
     1501 aaaaatcctg cagcggggcg agatagcgga gatttgtttc gccgatccac tgaatctgcc
     1561 ggacggggtg acggcgggca cgaaacgccg tctggatgag cttttcgcgg gaaaaccgcc
     1621 aagcgacctg tggtagccgc gtcccgcaac agcagtttgt ccgcttggcg ccattgattt
     1681 gcagtaatgt ttgcggatca atgccgcccg gccctggagg agttaatgcg cggcgcctgg
     1741 attgccccct tgttgttggg tttcgccctt gcgggctgcg gtggttccac gcctgcgccg
     1801 gctgaatcgt cgacggcggc cctcgagggc gacgccgcgt tgatagcctc cgcccaggtg
     1861 atcagcgacc agctgggcgg ctgtacgaaa ccagatggcg ccgcggtcga atcgaaagtc
     1921 gtcggcctgg agagcggccc gattgtcctg ctggcatgca gccaggacaa attcacttac
     1981 acgcatcgac tgttcgccat tcgcgccggg cagaaacccg aactcatttc gctgcccgac
     2041 tatgacgaca ggggcatgtt cgcgacggac caggcgagcc tcgcagagct cgacgctgga
     2101 acgggcgtgc tgacaacatt ccgcaaggcc gccgagcacg ggcgctgtgg atccgaagcg
     2161 cgctaccagt ggaacggcga acgcttcgac ctgcaggagt tgcactggca ggatt
//
LOCUS       DASUGB010000288         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_31915,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000288 DASUGB010000000
VERSION     DASUGB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31915"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFV87_05525"
     CDS             <1..392
                     /locus_tag="VFV87_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103247.1"
                     /translation="GSLLDLFDELVYFTRVFPHPNLTIETPLVEVEERRIPGHGKRRR
                     WGRRDHVIDDQRLTAVLSVHRFATTADLLALLPPELPQPFHTGELAEGLQIQRWIAQR
                     IAYCLRKTGAANVCGKRGNAILYQRAA"
     gene            580..1545
                     /locus_tag="VFV87_05530"
     CDS             580..1545
                     /locus_tag="VFV87_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103248.1"
                     /translation="MSADRMRATALVGGFLAALACAAPGLAEEGKCDCPARKTVAGIP
                     ILAKVPYVNRLFQAADTAGDDVQRIGIDFEFCADGEFPLGIRFWECGAACEGQCQAKA
                     GCAEGKCPVNVAACDCPVAKAVCPSCQVAAKACACASESKCAAASQCACGTKCACDTK
                     CACGDACQCSGQQVVVRSMGPVPFVTQHVPPHAVVVHHGPPHVGPAHPPVAACPAMFG
                     HLLELTAHSAALEAKLEARDEQAELVGEMLELAQENAKLKAQVEMADAKLELAEAQLE
                     ISRHLVQMKLENEHLKAQVAELTNRLGEAEKTARTTSETPSQPRR"
     gene            complement(1685..3409)
                     /locus_tag="VFV87_05535"
     CDS             complement(1685..3409)
                     /locus_tag="VFV87_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103249.1"
                     /translation="MTFYRLFLCPAAALAVLSLWISHLDAQNLKPVAVVSIASVEKNL
                     ADIVYLTRIVGMEDTGKTAQLFGNALTAGMDKSRPSGLVVIPSGQDFHAVAFVPVTDL
                     KLLLEVHKEQVGVPKDIGGSILEIGTDQKAYVKEQVGWAFIAEQQEHLAVLPADPAQL
                     LADLPAKYNVAVRLMAQNIPTELKQLAIDQMKAGIKQAAQGPAIPGAVPGRGPQPIPG
                     QPNPPAPAPDPQQAQMLEASMQQIERLINDAEDVTIGLAVNPEGKSVSLDLAVTAREG
                     TELAKTMAMQTGIKSSFSGFPLPEASLSFRATAAVSDADAALAKKNFDAQREQMKSAM
                     EILSKNATPEAADTVKKLTDQVFDLMAESAASKFTDQAMVLVLGPGSLNAAVGSYIPD
                     GAKLEQVLTEAAKSLNGQKAEDGSTIELVLNVGNHGDVKLHRGAMPASNAVPINKQLF
                     GEKMSFIIGTGKNSGYLAVGKDGETLLKKVIDRSAAQASQPVQPFQMTVSVVPILKFA
                     QSLQPNPLLAGLTASLEQEGGDKLLVTGTAGPRSIAYRIEVQEGIIRAGGQIGQMFAP
                     QFGAGLPQ"
     gene            complement(3613..>4118)
                     /locus_tag="VFV87_05540"
     CDS             complement(3613..>4118)
                     /locus_tag="VFV87_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103250.1"
                     /translation="KDLVELAKSDPNFPEVKFNVGQHSGVQLHSLEHAIPESEAEARE
                     LLGEKLNVMIGTGKNSFFISVGKDCESLLKKVIDRNAAEANKVSQPMQLNVSLLPILK
                     FYSSVDDNPLVPALAATLEQEGSDKLIVSSTAGPRSSTTRIEVQQGLVRVIGDAVKLI
                     RDSQLNQ"
BASE COUNT          703 a         1324 c         1295 g          796 t
ORIGIN      
        1 gcggcagcct gctcgacctg ttcgacgagc tcgtctattt cacgcgggtc tttccgcatc
       61 cgaacctcac gatcgaaacg cccttggtgg aagtcgagga gcggcgaatt cccggccacg
      121 gcaagcggcg gcgctgggga cggcgcgatc acgtgatcga cgaccagcgg ctgacagcgg
      181 tgctgtcggt gcatcgcttc gccaccaccg ccgacctgct cgcacttttg ccgccggaat
      241 tgccgcagcc gttccacacc ggcgagctgg ccgagggtct gcaaatccag cgctggattg
      301 cccagcggat cgcctattgt ttgcgcaaga ccggcgctgc caacgtctgc ggcaagcgcg
      361 gcaacgcgat cctgtaccag cgagcggcgt agggtgggac aaggccgccg cggccgcagg
      421 cccaccacgc aacgtcagcc gcggtgggcc tgcgctcgct tcgctcgctg gtcccaccca
      481 accgctagca cccattcgcc ttgtcgctgg ggcgcccagg cggtatatcc cgcaggcgat
      541 tctcgtctcg tctctccgtt tcaaggatgg aacatgaaca tgtctgcgga tcgaatgcgc
      601 gccaccgcgc tcgtcggtgg gtttctcgcc gccctcgcct gcgcggcgcc tggcctcgcc
      661 gaagagggta agtgcgactg cccggccagg aaaacggtcg ctgggattcc gattcttgcc
      721 aaggtgccgt acgtcaaccg cctgttccag gccgctgaca ccgccggcga cgacgtccag
      781 cggatcggca tcgattttga attctgtgct gacggcgaat tcccgctcgg catccgtttc
      841 tgggaatgcg gcgccgcctg cgaaggccag tgccaggcca aagctggctg tgcggagggc
      901 aagtgcccgg tcaatgtggc cgcgtgtgat tgtcccgttg ccaaagccgt ctgtccgtcg
      961 tgccaagttg cggcgaaggc ctgcgcttgc gcctccgaat cgaaatgtgc tgcagcaagc
     1021 cagtgcgcct gcggaacgaa gtgtgcctgc gacaccaagt gcgcctgcgg cgatgcctgc
     1081 caatgcagcg gccagcaagt cgtagttcgt tccatgggtc cggtcccgtt cgttacgcag
     1141 cacgtgccgc cgcacgccgt cgtcgtgcat cacggcccgc cgcatgtcgg cccggctcat
     1201 ccgccggtgg ccgcttgtcc ggccatgttt gggcacctgc tcgagctgac cgcccatagc
     1261 gccgccctgg aggcgaagct cgaggcccgc gacgagcaag ccgagctggt cggcgaaatg
     1321 ctggagctgg ctcaggaaaa tgcgaagctg aaggcccagg tcgagatggc cgatgccaag
     1381 ctcgagctgg ccgaagccca attggaaatc tcccggcacc tggtgcaaat gaagctcgag
     1441 aacgagcacc ttaaggcgca ggtcgccgag ctgaccaacc gcctgggcga agcggaaaag
     1501 accgcccgca ccaccagcga aacccccagc cagccgcggc ggtagcccgg cgtacggatc
     1561 ggaagggaca ggataatcgt aggacgggtt ttcaacccgt cccgcgtgga caggctggca
     1621 acctaaggac gggttgaaaa acccgtccta cgtcgcttct tggcaggctg gaagcctgtt
     1681 tcacttattg cggcaggccg gcgccgaact gcggggcgaa catttggccg atctggccgc
     1741 cggcgcggat gatcccttcc tgcacctcga tccggtaggc gatgcttcgc ggccccgctg
     1801 tgccggtgac cagcagcttg tcgccccctt cctgctccag cgacgcggtc agccccgcca
     1861 agagcggatt gggctgcagc gactgggcga atttcaagat cggcacaacc gagaccgtca
     1921 tctggaacgg ctgcaccggc tggctggcct gtgcggccga gcggtcgatc accttcttca
     1981 gcagcgtctc gccgtctttg ccgacggcca ggtagccgct gttcttgccc gttccaatga
     2041 tgaagctcat cttttcgccg aagagctgct tgttgatcgg cacagcattg ctggcgggca
     2101 tggccccgcg gtgcagtttg acgtcgccgt ggttgccgac attgagcacc agctcaatcg
     2161 tagagccgtc ttccgccttc tgaccattca acgacttcgc ggcttcggtc aggacctgtt
     2221 ccagtttcgc tccatcggga atgtagctgc cgaccgccgc gttgagcgat ccgggaccga
     2281 gcaccaggac catcgcctgg tctgtgaatt tgctagcagc cgattcggcc atgagatcga
     2341 agacctggtc ggtgagcttc ttcacggtgt ccgcggcttc gggcgtggcg ttcttggaaa
     2401 gtatctccat ggccgatttc atttgctcgc gctgcgcatc gaagttcttc ttggccagcg
     2461 cggcatctgc gtcggagacg gcggctgtgg cacgaaatga aagtgaggcc tcggggagtg
     2521 gaaagcccga gaacgaagac ttgataccgg tctgcatcgc catcgtcttg gccagttccg
     2581 ttccctcgcg agccgtgacg gccaggtcca ggctaacgct ctttccctcc ggattcaccg
     2641 ccaggccgat cgtgacatcc tccgcgtcat tgatcagccg ttcgatctgc tgcatcgagg
     2701 cttccaacat ttgcgcttgc tggggatccg gagccggcgc ggggggattc ggctgccccg
     2761 gaatcggctg cgggccgcga ccgggaacgg ctccgggaat cgccggccct tgcgccgcct
     2821 gcttgatccc cgccttcatc tggtcgatcg cgagttgctt caactcggtg gggatgttct
     2881 gcgccatcag ccgcacggcg acgttgtatt tcgccggcaa gtcggccagg agctgcgcgg
     2941 gatcggccgg cagcacggcc aggtgctctt gctgctcggc gatgaaggcc cagccgacct
     3001 gttccttgac gtacgccttc tggtcggtgc cgatttccag gatgctgccg ccgatatcct
     3061 tgggcacgcc gacctgctcc ttgtggactt cgagcagcag cttgagatcg gtgacgggta
     3121 cgaacgcgac ggcatggaag tcctggccgc tgggaatcac gaccagtccc gaggggcggg
     3181 acttatccat ccccgcggtg agcgcgttgc caaagagctg cgcagttttg cccgtgtcct
     3241 ccatccctac gatgcgcgtc agatagacga tatcggccag gtttttctcg acgctggcga
     3301 tcgacaccac cgccacgggc ttgaggtttt gcgcgtccag gtgcgaaatc cacaggctca
     3361 gaaccgccag cgcagcggcc gggcacaaga acaggcgata gaatgtcacg ggaaacctcg
     3421 gcaggaatag ggccagcgcc gatcgacagg gcgggagcac acgctcccgc cccacgcgat
     3481 tctcaactca gccagcgagg attgcaagcg tcaccgtagg cgactcgctc cgcgagtcgc
     3541 gatggacgtt gcagtttcag tatctattcg aatggaatgt ctatcgcttc tcacaaagcg
     3601 agaagcctac ggttactggt tcaactggct gtcgcgaatc agctttaccg catcgccgat
     3661 gacacggacc aggccctgct ggacctcgat ccgggtcgtg ctgctccgcg gtccggcggt
     3721 gctggagacg atcagcttgt cgctgccttc ttgctccagc gtggccgcca gagccggcac
     3781 cagcgggttg tcatcgaccg agctgtaaaa cttcaggatc gggagcagcg agacgttgag
     3841 ctgcatcggc tgggacactt tattggcttc ggccgcgttg cgatcgatca ccttcttgag
     3901 caacgactcg cagtctttgc ccaccgagat gaagaagctg tttttgcccg tgccaatcat
     3961 gacattcagc ttctcgccca acagctcgcg ggcctcggct tcggactcgg ggattgcatg
     4021 ctccagcgaa tggagctgca cgccgctgtg ctggcccacg ttgaacttta cttccgggaa
     4081 gttcgggtcg ctcttggcca gttcgacgag atccttga
//
LOCUS       DASUGB010000289         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1699447, whole genome shotgun sequence.
ACCESSION   DASUGB010000289 DASUGB010000000
VERSION     DASUGB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699447"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..2912)
                     /locus_tag="VFV87_05545"
     CDS             complement(336..2912)
                     /locus_tag="VFV87_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5103251.1"
                     /translation="MLESFNLLAELAPWQQSLLTWLGYDFSDVPTNARPELIWTNRPD
                     SWGVFVLIAVVAGVLYAVFSIYRRELDTCPRSVKLLLAALRAGVVLLLACIFLGPALV
                     YLKNRTIQPTIVLARDSSQSMNTPDRYTDEPAAKIASEALRKREGEIRTSRPTRVQIV
                     NDVLGYGGGQLLEQLGERGKIQTINFADQVTKIDLRQPKSPEDAAKGEGGSDQASGAG
                     DGPPGEEPIVAVPLLPPLVADGRGSDLAMAIRQALASDSPAAIILLTDGQHTGKDDPR
                     EAAREAKERGVPLLVVGVGDASRPRNLKVANLYVRPQVWQDEPFEIDAIVTAQGMEAG
                     EVRIELLEQRVGDNDAAIGSGTVVQSLQLPVPEGGGRLRAEFSHTAKESGRFVYTVRT
                     EAAADELAEDDNELTSAVVKVLSRERVRVLLVAGAPSWDYRLVQKLLARDKTVIISCW
                     LQTLDEERAQEGTRQITRLPITREELFYYDVVILMDPNPQEFDQAWIELLKQFVGEHS
                     GGLLFMAGPKHSGRLLTNSRTAELGKILPVSFGDVAAMEIAQITSTNQRAWPLKVVPA
                     NVDHPVMRFYPEREETLRRWETLPGIYWSFPTNDTKPTAEVLVEHSDPTLRQTEGSRP
                     LMVAGRYGSGHTLYLGFNGTWRWRKAGRQAEFFDKFWIQTIRYLVEGRSLEGRRRGYV
                     QTDRDRYEIGEKVTITARLQDATYNPLTLPKVDATLQTPGDSPETVPLLPIENQPGAY
                     EATLTVKKTGVHTVRITLPASDAESGIIESPFTVELPSVETNQVWLNKPLLVDLANQS
                     GGQYFDVNQLDQLAAAVPDKTEIIESRGPPDPLWDVRGMLIALVGLLCIEWLVRKRNK
                     LL"
BASE COUNT          515 a          957 c          924 g          581 t
ORIGIN      
        1 gaatggcatt cagatatcat ccaggatttt tccggggatg aaggaagcgc ataagtcatg
       61 ttatgaaaat acattacgtc gattttggcg cgtatgcgat tatgcgtttt atgcacttga
      121 ttcacgtttt cgccggatcg gatgaacagg ccctaaacag gggattccag ccaacgacca
      181 atccacttcc aggccccaca tttctgaccc ccgcatctat aaccgtccac gcattaaacg
      241 caggcgggtg gacacgtctg gtccatttca gtcaagtgaa aaacattatt ttcttgcacg
      301 cccctcgctt acgcttcggg tttcggtgtc aacggtcaca gcagcttatt ccgcttccgc
      361 accagccatt cgatgcagag cagcccgacg agggcgatga gcatcccgcg cacgtcccac
      421 agcgggtccg gcgggccgcg ggactcgatg atctcggtct tgtcgggcac cgcggcggcg
      481 agctggtcga gctggttgac gtcgaaatac tggccgcccg attggttcgc gaggtccacc
      541 agcagcggct tgttgagcca gacctggttg gtctcgacgc tcggcaattc cacggtaaag
      601 ggcgactcga tgatgccgct ttcggcgtcg ctcgccggca gcgtgatccg caccgtatgg
      661 actcccgtct ttttgaccgt cagcgtcgcc tcatacgccc ccggctggtt ctcgattggc
      721 aacagcggca ccgtttcggg cgagtcgccg ggcgtctgca gcgtggcatc gaccttcggc
      781 agcgtgagcg ggttatacgt ggcgtcctgc aggcgggcgg tgatcgtcac cttttcgccg
      841 atttcgtagc ggtcgcggtc ggtctgcacg tagccgcggc ggcgaccctc cagcgagcga
      901 ccctcgacga gatagcggat cgtctgaatc cagaacttgt cgaagaactc cgcctgccgg
      961 ccggccttgc gccaccgcca ggtgccgttg aagcccagat acaacgtgtg tcccgagccg
     1021 tagcgtccgg ccaccatcag cggtcgcgag ccctcagtct gtcggagcgt gggatcgctg
     1081 tgctcgacca gcacctcggc ggtcggcttg gtgtcgttgg tgggaaagct ccagtaaatg
     1141 cccggcaggg tctcccagcg gcggagggtc tcttcgcgct cgggatagaa ccgcatcacg
     1201 gggtgatcga cgttggccgg gacgaccttc agcggccagg cccgctggtt cgtcgaagtg
     1261 atctgcgcta tctccatggc cgcgacgtcg ccgaagctga ccggcaggat tttccccagc
     1321 tccgccgtgc gggagttcgt caagaggcgg cctgaatgtt tcggccccgc catgaacaag
     1381 agcccgccag agtgctcgcc gacgaactgc ttgaggagct cgatccaggc ctggtcgaac
     1441 tcctgtggat tggggtccat caggatgacg acgtcgtagt agaacagctc ctctcgcgtg
     1501 atgggcaaac gagtgatttg ccgcgtccct tcctgggccc gctcttcgtc gagcgtctgc
     1561 agccaacagg agatgatgac cgtcttatcg cgggccaaca gcttttgcac caggcgatag
     1621 tcccagctcg gggcccctgc caccagcagc acgcggaccc gctcgcggct gaggactttg
     1681 accacggcgc tggtcaactc gttgtcgtcc tcggccagct catcagcagc cgcttcggtg
     1741 cggacggtat agacaaagcg gcccgattcc ttggccgtgt gcgagaactc ggcccgcagg
     1801 cggccgcccc cttcaggcac cggcagttgc agcgactgga cgaccgtgcc gctgccgatg
     1861 gcggcgtcgt tgtcgccgac gcgctgctcc aagagctcga tgcggacttc gcccgcctcc
     1921 atgccctgtg ccgtcacgat cgcgtcgatc tcgaacggtt cgtcctgcca gacctgcggg
     1981 cggacataga gattggccac cttgagattc cgcggccggc tggcatcgcc gacgccgacg
     2041 acgagcagcg gcacgccccg ctctttcgct tcccgagccg cctcccgcgg atcgtccttg
     2101 ccggtgtgct ggccatccgt cagcaggatg atcgccgccg ggctgtcgct cgccagggcc
     2161 tggcggatgg ccatcgccag gtccgaaccg cggccatcgg ccaccagcgg cggcaacagc
     2221 ggcacagcga caatgggctc ttcgcccggc gggccatcgc ctgcgccgct ggcctgatca
     2281 ctgccgcctt cacctttcgc ggcatcctcc ggtgattttg gctgacggag gtcgatcttc
     2341 gtcacctggt cggcaaagtt gatcgtttga atcttgcccc gctcgccaag ctgctccaga
     2401 agctgcccgc cgccgtagcc gagcacatcg ttgacaatct gcacgcgcgt cggacggctc
     2461 gtacggattt cgccctccct ctttcggagc gcttcggaag cgatcttcgc agccggttcg
     2521 tcagtataac ggtccggcgt gttcatcgac tgcgaggagt cgcgagccag cacgatggtg
     2581 ggctgaatcg tgcggttttt cagatagacc agcgccggcc cgaggaagat gcacgccaag
     2641 agcagcacca cgccggcccg caatgcggcc agcaggagct tcacgcttct cgggcaggta
     2701 tcgagctccc ggcgatagat cgaaaacacg gcatacagta cgccggccac gaccgcgatc
     2761 agcacgaaca caccccagct atcgggacgg ttcgtccaga tcagctcggg acgggcattg
     2821 gtgggcacgt cgctgaagtc gtagcccagc caggtcagca aggattgctg ccagggggcc
     2881 agttcggcga gaaggttgaa cgattcgagc atttgtggat gtcagtaccg ccacatgcgg
     2941 ctgaggtttg gcatttccag tcggaagaca gtggttc
//
LOCUS       DASUGB010000290         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1421039, whole genome shotgun sequence.
ACCESSION   DASUGB010000290 DASUGB010000000
VERSION     DASUGB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421039"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFV87_05550"
     CDS             complement(<1..371)
                     /locus_tag="VFV87_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5103252.1"
                     /translation="MLAFAIRFRLRAAAIALAFASLLPAARAEEIKNSAGMTLTLIEA
                     GRYERGERDMGRDFAKDHVEFNTAEDDRPVHPVVLTKPFYLATTEVTIAQFRQFARAA
                     GYKTTAENNPRGAVGWDPTPPA"
     gene            complement(388..1710)
                     /locus_tag="VFV87_05555"
     CDS             complement(388..1710)
                     /locus_tag="VFV87_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5103253.1"
                     /translation="MSRKLLDRRTVLKSLGALVPLPLLEAMLPGTNRGADAASPVPPR
                     MLVCHFGTGMNIREFFPKETGPDCTLPRIVKPLEAWRKRMTIVSGVQLEHGGGHTGDY
                     TFLTGTGGWKSTGIKSGISADQVVAGEIGGQTRFASLQLSIARGTNYGQQGLATLSWN
                     KNGIPLAAENDPHAIFQRLFGIDDAREAAGRAAGFRRRASILDYASGQARQMQRAVGV
                     ADRLKLDEYFTAVREIESQLERDVAWSQKPKPQPQLDGLGDYSRSLTHQTTGFDYAVY
                     QKLMYDLIALAFQTDSTRVITYNVRQELSGGTFAVHGVSKNFHSLTHHNNEPKNLDEL
                     AQVDEINMGFWTKFLERLEQIPQPDGRSLLEHTVIAYSSSAGMDHSRDKLPTAIFGGE
                     ALGMKHSTHLALAAGTPLARVWHTLADRMGVQRDTLQDSRGPISELLA"
     gene            complement(1762..3132)
                     /locus_tag="VFV87_05560"
     CDS             complement(1762..3132)
                     /locus_tag="VFV87_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5103254.1"
                     /translation="MNTNDREPNADLVRLTESFCGGQLSGEEWARLERQLLADPEARA
                     YFRRHASLDAALRDYRQSAAESWHSNVSSASSPSQALVQPARRTSRFALWAAAAASAA
                     LCVCAAWLLLGRTSEGRVIASLEEVTGDVRVLDASGQVRPIVADAAQIRSGDTVRTSG
                     SRSATVVACADGTRLTLVGNTSVTCGQSSGMSFVIHHGTLAAAVQPQPRKLPLVLATP
                     TAQVEVLGTRLLIEAGQRRTDLNVTEGRVRLVRVKDGETIEVAGGKRAVVTDQEQLLV
                     EDIPRLADAWEVDFEDGLPAGWEIGEHTAQNLPPHSRGAVRAARHEGDDGETYHSIVS
                     GSAWSQGLFAVGKNSHLHVTMKMDNPQWLNLFLITRTADPQNPRFCSNFIFKAFPKLL
                     PGRWQTISIPLASFRKLSAGPETLEQVVPYHVIFNSTGPDRGLVIDRIWVTPDGTGEV
                     SLQDAE"
     gene            complement(3129..3683)
                     /locus_tag="VFV87_05565"
     CDS             complement(3129..3683)
                     /locus_tag="VFV87_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5103255.1"
                     /translation="MSTEHHQPRVQPEDSYERFVRLFARHEPALRSFVRPLVPHWDDA
                     DEVLQQTCVVLWRKFAEFDTGSDFLAWACTIARFEVLKHRRRIARDRHVFSEELLQLL
                     ADEGAAEADARQRERRALDHCLELLPAPQRELIQRCYGDAGAINAAAAALGRSATSVY
                     KALQRVREVLLECIERRLAQEAIP"
BASE COUNT          625 a         1249 c         1245 g          682 t
ORIGIN      
        1 gctggcgggg ttgggtccca gccgaccgcg ccccgcgggt tgttttccgc ggtagtcttg
       61 tagccggcgg cgcgggcgaa ctggcgaaac tgggcgatgg tgacctcggt cgttgcaaga
      121 tagaagggct tcgtcaaaac caccggatga acggggcgat cgtcctcggc tgtgttgaat
      181 tcgacgtggt ccttggcaaa atcgcgcccc atgtcgcgct cgccccgctc gtagcgaccg
      241 gcttcgatca gcgttagcgt catgccggcg gagtttttaa tttcctccgc ccgcgcggcc
      301 ggcaacagcg aggcaaaagc cagtgcgatg gccgcggcgc gcagacggaa gcgaattgcg
      361 aaagccagca tcggtcggtt ccgtgattta ggcgagcagc tcgctgatcg gtccgcggct
      421 gtcctgcagg gtgtcgcgct ggacgcccat gcggtcggcc agcgtgtgcc acacgcgggc
      481 cagcggcgtg cccgcggcaa gggccaggtg cgtggaatgc ttcatcccca aggcttcgcc
      541 gccgaagatc gctgtcggca gcttgtcgcg cgagtgatcc atccccgcgc tcgacgaata
      601 cgcaatgacg gtgtgctcca gcaggctgcg gccatcgggc tgcggaatct gctccagccg
      661 ctcgaggaac ttcgtccaga agcccatgtt gatttcatcg acctgtgcca gctcgtcgag
      721 gtttttgggc tcgttgttgt ggtgggtcaa ggaatggaag ttcttcgaga caccgtggac
      781 ggcgaacgtg ccgccggaga gctcctgccg cacgttgtag gtgatcacgc gggtcgaatc
      841 ggtctgaaac gccagggcga tcaggtcgta catcagcttc tgatagacgg cgtagtcgaa
      901 gcccgtcgtc tggtgcgtga gcgagcgcga gtaatcgccc aggccgtcca actgcggctg
      961 cggcttgggt ttttgcgacc aggcgacatc gcgctctagt tggctctcga tttcgcggac
     1021 ggcggtgaag tactcgtcga gcttcaggcg atcggcgacg ccgaccgcgc gctgcatctg
     1081 tcgggcctgg ccgctggcat aatcgaggat gctggcccgg cggcggaagc cggccgctcg
     1141 gccggccgct tcgcgggcgt cgtcgatccc aaacagccgc tggaagatcg cgtgcggatc
     1201 gttctcagcc gccagcggaa tgccgttctt gttccacgac agggtcgcca gcccctgctg
     1261 gccgtagttc gtgccacggg cgatcgaaag ctgcagcgag gcgaaccgcg tctggccgcc
     1321 gatttcgccg gcgaccacct ggtcggccga gatgccgctc ttgatgccgg tcgatttcca
     1381 gcctccggtg ccggtgagga aggtgtaatc gcccgtgtgc ccgccgccat gttcgagctg
     1441 cacgcccgaa acgatcgtca tccgcttccg ccaggcctcc agcggcttga cgatccgcgg
     1501 cagcgtgcaa tccgggccgg tttccttggg gaagaactcg cgaatgttca tgcccgtccc
     1561 gaaatgacag acgagcatcc gcggcggcac ggggctggcg gcatccgccc cccggttggt
     1621 gccgggcagc atcgcctcca agagcggcag cgggacgagc gcccccagag acttcaggac
     1681 ggtgcggcga tcgagcagtt ttcgcgacat ggcatcctca ggcttgaagg caaggcttca
     1741 tcgtactcga aacttttcga ctcattccgc atcctggagc gagacctcgc cagtaccatc
     1801 cggcgtgacc cagatccggt cgatgacgag cccgcggtcg ggacccgtgg aattgaaaat
     1861 tacgtgatag ggaaccacct gctcgagcgt ctccgggccc gcactcagct tgcgaaagct
     1921 cgccagcggg atgctgatcg tctgccaccg gcccggcagc agcttgggaa acgccttgaa
     1981 aatgaagttg ctgcagaatc gcggattctg cggatcggca gtgcgcgtga tcaggaacag
     2041 attcagccac tgcgggttgt ccattttcat cgtcacgtgc aggtgcgagt tctttcccac
     2101 ggcgaatagc ccctgcgacc aggccgaccc cgaaacgatg gaatggtacg tctcgccgtc
     2161 gtcgccctcg tggcgggcgg cccgcacggc gccgcggctg tgcggcggga gattctgcgc
     2221 tgtgtgctcg ccgatttccc agcccgccgg caatccgtct tcaaaatcga cctcccacgc
     2281 atcggccagg cgcggaatgt cttcgacgag caattgctcc tgatcggtga cgacggcccg
     2341 cttgccgccg gccacttcga tcgtctcgcc gtccttcacg cgcaccagcc gcacacggcc
     2401 ctccgtcacg ttcaggtccg tccgcctctg gccggcctcg atcagcagcc gcgtgccgag
     2461 cacttcgacc tgcgccgtcg gcgtggccag cacgagcggc agcttgcgag gctgcggctg
     2521 aaccgcggcg gccagcgtgc cgtgatggat cacgaagctc ataccgcttg attggccgca
     2581 ggtgacggaa gtgttaccga ccagtgtcaa ccgcgtgccg tccgcacagg cgacgaccgt
     2641 ggcgctgcgg ctgccgctgg tccgcacggt atcgcccgag cggatctgcg ccgcgtccgc
     2701 cacgatcggc cgcacttgcc cgctggcatc gagcacgcgg acatcgccgg tcacttcttc
     2761 cagcgatgcg atgactcgcc cttcggacgt tcgcccaagc agcagccatg cggcacagac
     2821 gcaaagtgcg gccgaagccg ccgccgctgc ccacagcgca aagcgggatg tgcgccgggc
     2881 cggctgcacg agagcctggc ttggggatga agcggacgaa acattggagt gccaggattc
     2941 cgcagcgctc tgccgatagt cgcgcagcgc cgcatcgagg ctggcatgcc ggcggaaata
     3001 ggcccgcgct tcgggatcgg ccagcaactg ccgctcgagc cgggcccatt cctcgccgct
     3061 gagctggccg ccgcagaacg actcggtcag ccgcacgagg tcggcattcg gttcgcgatc
     3121 gttggtgttc atgggatcgc ctcctgggcc agcctccgct cgatgcactc cagcaggact
     3181 tcgcgcacgc gttgcagggc cttatagacg ctggtcgcgc tgcgcccgag cgccgcggca
     3241 gccgcgttga tcgcgccagc atcgccgtag cagcgctgga tcagctcgcg ctgcggcgcc
     3301 ggcagcagct ccaggcagtg gtccagcgct cgccgctcgc gctgccgcgc gtccgcttcg
     3361 gcagcaccct cgtcggccaa tagttgcagc aattcttcgc tgaacacgtg gcggtcgcgg
     3421 gcaatccgcc ggcggtgctt gagcacttcg aaacgggcga tcgtgcaggc ccaggccaaa
     3481 aagtcgctgc ccgtatcgaa ttctgcgaat tttcgccaca gcacgacgca tgtctgctgc
     3541 agcacctcgt cggcatcgtc ccaatgcggg accagcggcc gaacgaacga ccggagggcc
     3601 ggctcatgcc tggcaaatag ccgcacaaat cgctcgtaag agtcttccgg ctgtacgcgt
     3661 ggctggtggt gttcggtgct catggcatgg ctgtccgttc gagcggggct gctttctcgc
     3721 acgtatttac tcccgcccgg ccaagaattg ccctccaacc ggtagaaatt gaggcagcgc
     3781 caaagacgag ttcttcggaa t
//
LOCUS       DASUGB010000291         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_449463, whole genome shotgun sequence.
ACCESSION   DASUGB010000291 DASUGB010000000
VERSION     DASUGB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449463"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VFV87_05570"
     CDS             complement(<1..1114)
                     /locus_tag="VFV87_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103256.1"
                     /translation="MFQINKTFSNCQGLSRRPFLQVGALSLFGLTLPKLLAARAAQGA
                     EAGRDMNCILLFTAGGMSNVDTLDMKPNAPIEYRGEFKSLPTKIPGQLACEHLPRMAD
                     CLDKVCLVKSIVHAESGDHAAATHYMLTGYPQRPDPTGQPAGSTIYPMFGSVVAKERG
                     WKQGMPANVQVGEQISYSGAGYLGSRYSPLAIRTDPNAADFSVEDVSIPAAIGEPRTA
                     RRQRLLSRLDAWQQSTEKNAGSVLDRSEFYRQAFSLMTSPAAKKAFNIKEEPDKVRDR
                     YGRNATGQGVLLARRLIESGVRFVSVISGGWDTHDNNFGRLKTTLLPTLDQCWSALIE
                     DLSQRGMLDSTLVICAGEFGRTPKVNAAAGRDHYAPC"
     gene            complement(1124..3352)
                     /locus_tag="VFV87_05575"
     CDS             complement(1124..3352)
                     /locus_tag="VFV87_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103257.1"
                     /translation="MMNRHRAITDLFASGVLLAAFFLAADVACGAEPPPHFIRDVVPV
                     LTKTGCNAGACHGSFQGRGGFRLSLLGFDPPADYEAIFKGARGRRVSLGSPESSLLMQ
                     KALGAVPHGGGRRIEPGSASHQVLLGYLKTGAIVPSDWKLHVQTLTAEPKTVTLALGS
                     SVTAQSRARWSDESEKSVSAWALYDSSDPLVAEVSAAGEIKAISPGKANVMLRFAGQV
                     AAVEVTVPYGPPPELAQFKPVNYIDELVAAEWKRLGVTPAEVCSDQDFVRRVYLDLIG
                     TLPRPEEVKKFLASADADKRGKLIDELLDRPEYVEFWALKWGDLLRGHKRYLGERGLG
                     SFTSWVRKSLRENKPLDQFTKEILTSQGNLYTSGPVAYYFVDSTPEELAETTAQVFLG
                     VRMQCARCHHHPLEVWSQDDYYGLAAFFNRVEAKNSGDNGRFGGLKSIRAVAAPVRDR
                     GTRPEVKPKMLGEPVVIASTTQTSATPVAASPAEPDVRLKLADWIANPDNPYFARNFA
                     NRYWSYLIGRGIVEPIDDLRATNPPSNAALLDALTKDFTDHKFDVKHLLHTICRSRVY
                     QLASERAPTRDQDGSLFTHRSLKRLSAEVLLDGVNQVCETSETFAGLPEGTRAISLPD
                     PTTASYFLTTFGKPLRTTACECGRMAGPDLSQALHLANSPALQAKLSNDKGRIKRLIA
                     EKKTDPEIADELYLATFARSPSDGERKVIAETLAEIPSRQEAWEDVLWSLLNSSEFVF
                     QH"
BASE COUNT          643 a         1106 c         1129 g          676 t
ORIGIN      
        1 tgcaaggagc gtagtgatcc cgcccggcag ccgcgttgac tttgggcgtg cgtccgaact
       61 cgccggcgca gatgacgagc gtggaatcga gcatgccgcg ctgcgagagg tcctcgatga
      121 gcgccgacca gcactggtca agcgtcggca ggagcgtcgt cttcagccgc ccgaagttgt
      181 tgtcatgcgt gtcccaaccg ccggaaatca cggagacaaa ccggactcca gactcaatca
      241 gccgccgagc cagcagcact ccttgccctg tcgcattgcg gccatagcgg tcgcggactt
      301 tgtccggctc ctccttgatg ttgaaagcct tcttggcggc tggcgaagtc atcaggctga
      361 atgcctggcg atagaattcg ctccggtcga ggacgctgcc ggcattcttc tcggtgctct
      421 gctgccaggc gtcgagccga gaaaggagtc gctggcggcg ggcggtgcgt ggttcgccaa
      481 tcgccgcggg gatgctgacg tcctcgacgc tgaagtcggc ggcgttggga tcggtccgaa
      541 tcgccagcgg actataccgc gagccgagat agcccgcgcc ggagtaggaa atctgctcgc
      601 cgacctggac gttggcgggc atgccctgtt tccagcctcg ctctttggct accacgctgc
      661 cgaacatcgg gtaaatcgtc gatccggcgg gctggccggt cgggtcgggg cgctgcggat
      721 agcccgtgag catgtagtgc gtggcggccg cgtggtcgcc gctctcggcg tggacaatcg
      781 acttgacgag gcagaccttg tcgaggcagt cggccattcg cggcagatgc tcgcacgcga
      841 gctggccggg aatcttcgtc ggcaggcttt tgaactcgcc gcggtactcg atcggtgcat
      901 tcggcttcat gtcgagcgta tcgacgttgc tcattccgcc ggctgtgaag agcagaatgc
      961 agttcatgtc gcggcccgct tcggcgcctt gtgctgcccg cgcggcgagg agcttcggca
     1021 gcgtgaggcc gaagagcgag agggcaccga cctgcaggaa gggacggcga ctgaggccct
     1081 ggcaattgga gaaggttttg ttgatttgga acatggcaaa gggctaatgc tgaaacacaa
     1141 actccgagct attgaggagg gaccaaagga catcctccca cgcttcctgc cgcgacggaa
     1201 tctccgcaag cgtctctgcg attacctttc gctcgccgtc gctcggagag cgggcgaacg
     1261 tggccagata cagctcatca gcgatctctg gatcggtctt cttctccgcg atcaatcgct
     1321 tgatccgccc cttgtcgtta ctgagcttgg cttgcaaagc cgggctgttg gccaggtgca
     1381 gcgcctgcga caggtccggc cccgccatcc gcccgcactc gcaggccgtc gtccgaagcg
     1441 gcttgccgaa ggtggtcaaa aagtaagagg cggtcgtcgg gtccgggagc gagatggctc
     1501 gcgtcccttc cggcagcccg gcgaaggtct cgctcgtttc gcaaacctgg ttgacgccgt
     1561 cgagcagcac ttccgcagaa agtcttttga gcgagcggtg ggtaaagagc gagccatcct
     1621 ggtcgcgggt cggcgctcgt tccgaggcga gttgataaac tcgtgagcgg cagatggtgt
     1681 ggaggaggtg tttgacgtca aacttgtggt cggtgaagtc cttggtaagt gcgtcgagga
     1741 gcgcggcgtt gctcggcgga ttcgtcgctc gcagatcgtc gatcggctcg acaatgccgc
     1801 ggccgatgag atacgaccaa tagcgattgg cgaaattgcg ggcaaagtac ggattgtccg
     1861 gattggcaat ccagtccgcc agcttcaggc gaacgtccgg ctccgcgggg gacgcagcga
     1921 ctggagtcgc cgatgtttga gttgtactgg cgatgacgac cggctccccc agcatcttcg
     1981 gcttcacttc cggccgcgtg ccgcggtccc gcacgggagc ggcgacggcg cgaatcgact
     2041 tgagtccgcc gaagcggccg ttgtcgccac tgttcttcgc ttcaacccgg ttgaagaacg
     2101 cggcgaggcc gtagtagtcg tcctgactcc aaacttccag cggatgatgg tggcagcggg
     2161 cacactgcat gcggacgccg aggaacacct gagccgtcgt ctcggcgagc tcctccggcg
     2221 tgctatcgac gaagtaatag gcgaccgggc cgctggtgta gagattcccc tgcgacgtca
     2281 gaatctcctt cgtaaactga tcgagcggct tattttcgcg cagcgatttt ctgacccacg
     2341 acgtgaagct gccgaggccc cgctcgccga gataccgctt gtggccgcgg agcagatcgc
     2401 cccacttcag cgcccagaac tcgacatact ccggccgatc cagcagctcg tcgatgagct
     2461 tgccgcgctt gtcggcatcg gccgaggcca ggaacttttt cacttcctcc ggccgcggca
     2521 gcgttccgat gaggtccaaa tagacgcgcc gcacgaaatc ctgatcgctg caaacttccg
     2581 cgggagttac gcccaggcgc ttccactcag cggccacgag ctcgtcgatg tagttgactg
     2641 gcttgaattg cgcgagctcg ggcggcgggc cgtaggggac cgttacctcc actgccgcta
     2701 cctggccggc aaagcggagc atcacgttcg ccttccccgg gctgatcgcc ttgatttcac
     2761 cggcggcgct cacttcggcg accagcggat cgctggagtc gtaaagcgcc cacgcagaaa
     2821 cggacttttc gctttcgtcc gaccagcggg ctcgcgactg ggcagtcacg ctcgagccaa
     2881 gggccagcgt caccgttttc ggctcggcag tcagcgtctg aacgtgcaac ttccagtcgc
     2941 tgggaacgat tgctccggtt ttcaagtatc ccagcaacac ctggtgcgaa gctgatcccg
     3001 gctcaatccg ccggccgccc ccatgcggaa cagcacctag agccttctgc atcagcaggc
     3061 tgctctcagg gctccccagc gagactcgcc gcccccgagc tcctttgaaa atcgcctcat
     3121 aatcggcagg cggatcaaac cccagcagtg acagccgaaa tccaccccgc ccctgaaacg
     3181 acccgtggca agccccggca ttgcaccccg tcttggtcag gacgggcacc acatcccgaa
     3241 tgaaatgggg cgggggctct gctccgcagg caacgtccgc ggccaaaaaa aacgccgcca
     3301 gcaggacgcc actggcaaac aaatcggtga tggcccggtg cctgttcatc atgctgttcc
     3361 gacaagtcgt aacatcaacc gctgcttatg tttgttgtaa gctgggcttc atgcaatagc
     3421 aagggaaaca ggctcgcctc gctccgttct ggtttccccg ttgggtcggc gacgagcgcg
     3481 gttgacgaac ctcgccccct gtcgcaaact ctaaacaggc attggccaaa gaggtcaggt
     3541 gcgcgagagt ggcg
//
LOCUS       DASUGB010000292         4331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1699725, whole genome shotgun sequence.
ACCESSION   DASUGB010000292 DASUGB010000000
VERSION     DASUGB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699725"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VFV87_05580"
     CDS             <1..1451
                     /locus_tag="VFV87_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103258.1"
                     /translation="DYKTLPAYSTSFFPLAYLCAGKPIPEQADRSIRALLVQDDDGYM
                     NNHIAATFHASHYYSLVGETTPKAAQMVARTLRDQKPDGSWLLHMPSRDRHATFDAVF
                     TLKHEGQGRPDCREAIDRAAKWALACRNSDGGFGHFPGSTSDADAVYFQVGTLVMAGF
                     LQPADPLPADPHLLSWGHLMPLRAKVPGDPSQIAVSGWVGGVALNDGGFNLATGSSDG
                     IARLWDASSGKRLADYQGHSDRVAAVAFSPDGSWLASASYDRTVKIWSLNSDKALRTL
                     TGHRGAVLCVALAYGEKEEFIATGSVDQTIRVWNLNGQHRRTLVGHKSWVNSVAFGPD
                     AKRLYSGSSDGTVKVWSVAGELLKSLDASKAEVRSVAISPDGKHLAAGLRYGAVKTWN
                     TGNWDLKQSWEHIADDAWSVTFSADSQRLLIAAGEWNRPTTIVERNVATGEIVREYKH
                     PGEALCLAISADGKTIAAGGGDKTVSVWKADP"
     gene            complement(1426..2634)
                     /locus_tag="VFV87_05585"
     CDS             complement(1426..2634)
                     /locus_tag="VFV87_05585"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX5103259.1"
                     /translation="MSAALEFRGNVILPDRVLEGGAVRAQAGRIVAVGPADGPGSPDN
                     VIDAGDGYIAPGFVDLHVHGGAGADFMDGTEEAFLTALACHARHGTTSLAATTTVARH
                     EQIMAVLEVARQLRKREGAPGSRVMGVHFYGPYFRKEAKGAHPGAAIRPPVAEEYLQY
                     LEYADSLVTCTVAPELPGAKDFALACRQRGVRTNVGHSWATFDQMAQAVEWGVRHVDH
                     LFCAMSDKTRLRQFQMYPMQGGVLEATLFFDELTTEVIADGKHLSHDLLKLALKIKGP
                     DRLALVTDCSRALDMPDGEYLIGPLDGGEPIIKLDGVGMLPDMKALGSSIRGMDFMVR
                     TFLELTGRPLWEVVRMATLTPARIAGWEREIGSLETGKRADVLVLSRDLEAQRVFRDG
                     VEITDRPSTR"
     gene            complement(2631..>4331)
                     /locus_tag="VFV87_05590"
     CDS             complement(2631..>4331)
                     /locus_tag="VFV87_05590"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103260.1"
                     /translation="GFPLLTDPAGVVADQFGAKRTPEAFVLDGKGIVRYWGRIDDQYG
                     IGYSRNEPTKSFVAQALDDLLAGKEVAPPSQEPVGCYIGRVRRGVPTGDITYTKHVAG
                     ILQKNCVRCHRPGEVAPFSMTGYEDAAAWAETIVEVIDDGRMPPWHANPQHGKFYNEA
                     RLSDDQKKLIRTWVANGCPQGDPADLPEFKPFTDGWQIPQPDIVYKMPEPFNVPASGT
                     VEYQFFTIDPGFTEDRWIKAAECRPGNRAITHHLILFFQEPGKPFEPIDALFNSIVGF
                     APGLPPAIYPEGIYRRIPAGSKLVIQAHYTPNGTPQSDQSEVGIVFADPKEVKKEMLV
                     AAALNFKFLIPPGEANFPLEATDKIGQDSVLYAMTPHMHLRGKSFRFNALYPDGHEEI
                     LLDVPRYDFNWQNSYALAEPKFLPEGTQIRCQAVFDNSEDNLANPDPASSVHWGDQTW
                     EEMMVGTMAIGMAEQDLSLGVPKSKKLDSGDYEVTFAYKPPTETEAVYLAGTFNDWKD
                     TGHKMDGPDADGRYTTTLTLKPGSHEYKFVIDGKRWKSDPGNPHTAGYFRNSVLKVGP
                     "
BASE COUNT          753 a         1402 c         1363 g          813 t
ORIGIN      
        1 aggactacaa gacgcttccg gcttattcga ccagcttctt tccgctggcc tacctctgcg
       61 ccggcaagcc aatcccggag caggccgacc gctcgatccg cgcgctgctg gtacaggacg
      121 acgacggcta tatgaacaat cacatcgccg cgacatttca tgcgtcgcac tattactcgc
      181 tcgtcggcga aacgacgccc aaggctgcgc aaatggtcgc ccgaacgctt cgcgaccaga
      241 agcccgacgg aagctggctg ctccacatgc cctcgcgcga ccggcacgcg acgtttgacg
      301 ccgtctttac gctcaagcac gaagggcaag gcaggcccga ttgccgcgaa gcgatcgacc
      361 gcgccgccaa gtgggccctc gcctgtcgca actccgacgg cggcttcggc cacttccccg
      421 gttcgacttc cgatgccgac gccgtctatt tccaagtcgg cacgctcgtg atggccggat
      481 tcctgcagcc tgccgacccg ctgccagccg atccgcacct cctctcctgg ggccacctga
      541 tgccgctgcg agcgaaggtg cctggcgacc cctcgcaaat cgccgtgtca ggttgggtcg
      601 gcggcgtggc gctcaatgac ggtggattca acctcgccac gggatcttca gacggaatag
      661 cgcgattgtg ggatgctagt tcgggcaaac gattggccga ctaccaaggg cactccgacc
      721 gtgttgctgc cgtagcgttt tccccggatg gaagctggct tgcctcggcg agctatgatc
      781 ggactgtcaa aatctggagc ctgaattctg acaaggcatt gaggactctt actggccacc
      841 gcggagctgt gctgtgtgtg gcacttgcgt atggtgaaaa agaagagttc attgctacgg
      901 gcagcgtcga tcaaaccatt cgcgtttgga atctcaacgg tcagcatcgg cgcacactcg
      961 ttggccataa gtcgtgggtg aattcggttg cgtttggtcc ggacgcaaaa cggctctact
     1021 ccggcagctc cgacggcacc gtgaaggtct ggtccgtcgc cggggaattg ttgaaatctc
     1081 tcgacgccag caaagctgaa gtacgctccg tggcgatttc tcccgatggt aagcatctgg
     1141 ctgccgggct ccggtacggt gcggtgaaaa cttggaacac cggcaactgg gacctgaagc
     1201 aaagctggga acatatcgct gacgatgcct ggtcggtgac attcagcgcc gatagccagc
     1261 ggctgctgat agcggccggc gaatggaacc ggccgacgac gatcgtggaa cggaatgtag
     1321 cgaccggcga aatcgtgcgc gagtacaagc acccgggcga agcgctctgc ctggcgatct
     1381 ccgcggacgg caagacgatc gctgcgggtg gcggcgacaa aacggtcagc gtgtggaagg
     1441 ccgatccgta atctccaccc cgtcccgaaa gacccgctgc gcctccaggt cacggctgag
     1501 gacgaggacg tcggcccgct tgccggtttc gaggctgccg atttcgcgct cccagccggc
     1561 gattcgcgcc ggcgtgagcg tcgccatgcg gacgacctcc cacagcggcc ggccggtcag
     1621 ctccagaaac gtgcggacca tgaagtccat gccgcgaatg ctggagccca gggccttcat
     1681 gtcgggcagc atcccgacgc cgtcgagctt gatgatcggc tcgccgccgt cgagcgggcc
     1741 gatcaggtat tcgccgtccg gcatatccag cgcccggctg caatcggtga ccagcgccag
     1801 ccgatccggc cccttgatct tgagagccag cttgagcaag tcgtgcgaga ggtgcttgcc
     1861 gtcggcgatg acttcagtcg tcagctcgtc gaagaagagc gtcgcttcga gcacgccgcc
     1921 ctgcattgga tacatctgaa actgccgcag gcgggttttg tccgacatgg cgcagaacag
     1981 gtgatcgacg tgccgcacgc cccactcgac cgcctgggcc atctggtcga acgtggccca
     2041 ggagtgcccg acgttcgtcc gcacgccgcg ctggcgacag gcgagggcga agtccttggc
     2101 tcccggcaat tcgggcgcaa ccgtgcaggt gacgagcgaa tcggcatact ccaggtactg
     2161 caggtactcc tcggcgaccg gcgggcgaat cgccgctccc gggtgcgccc ccttcgcttc
     2221 tttccgaaag tagggtccgt agaaatgaac ccccatcaca cgcgaacccg gtgcgccctc
     2281 acgctttcgc agttgccgcg cgacctccag caccgccatg atctgctcgt gccgggccac
     2341 ggtcgtcgtc gcggctaggc tcgtcgtccc gtgccgggcg tggcatgcca gcgcggtaag
     2401 gaacgcctct tccgtcccat ccatgaaatc cgcgccggcc ccgccatgca cgtgcagatc
     2461 gacaaagcca ggagcgatat agccgtcgcc ggcgtcgatg acattgtccg gcgatcccgg
     2521 cccgtcagcc ggaccgacgg cgacaatcct gcccgcctgc gcgcgaaccg cgccgccctc
     2581 cagcacgcgg tccggcagga tcacgttgcc gcggaactcc agcgccgcac tcacggcccc
     2641 accttcagca cgctgttgcg gaaatacccg gccgtgtgcg gattgccggg gtcgctcttc
     2701 caccgcttgc cgtcgatgac gaacttgtac tcgtggctgc cgggcttgag ggtcaaggtg
     2761 gtcgtgtacc ggccgtcggc gtccggccca tccatcttgt ggccggtgtc cttccagtcg
     2821 ttgaaggtgc cggcgagata gacggcttcg gtctcggtcg gcggcttgta ggcgaaagtg
     2881 acctcatagt cgccgctgtc gagcttcttc gacttgggca cgcccagcga caggtcctgc
     2941 tcggccatgc cgatcgccat ggtgccgacc atcatctctt cccacgtctg gtcgccccag
     3001 tggaccgacg acgcgggatc gggattcgcc aggttgtcct ccgagttgtc gaacaccgcc
     3061 tggcagcgaa tctgcgtccc ttcgggcagg aatttcggct ccgcgagggc gtacgaattc
     3121 tgccagttga aatcgtaccg cggcacgtcg agcagaatct cctcatggcc gtcgggatag
     3181 agcgcgttaa agcggaacga cttgccgcgg aggtgcatgt gcggcgtcat ggcgtagagc
     3241 accgaatcct ggccgatctt gtcggtcgct tccagcggga aattcgcctc gcccggcgga
     3301 atcaggaatt tgaagttgag cgctgccgcg acgagcatct ctttcttcac ctccttggga
     3361 tcggcgaaga cgatgcccac ttcgctctgg tcgctctgcg gcgtgccgtt gggcgtgtaa
     3421 tgtgcctgga tcacgagctt tgagcccgcc ggaatccgcc gataaatccc ctccgggtaa
     3481 atcgccggcg gcaatcccgg cgcaaagccg acgatcgagt tgaacagcgc gtcgatcggc
     3541 tcgaagggct tgcccggctc ctggaagaag agaatcaggt ggtgcgtgat cgcgcggttg
     3601 ccggggcggc actcggcggc tttgatccac ctgtcttccg tgaagcccgg gtcgatggtg
     3661 aagaactggt attcgacggt gcccgacgcc ggcacattga acggttcggg catcttgtag
     3721 acgatgtcgg gctgagggat ctgccagccg tcggtgaagg gcttgaactc ggggaggtcc
     3781 gccggatcgc cctgtgggca gccgttggcg acccacgtgc ggatcagctt tttctggtcg
     3841 tcgctcagcc gggcctcgtt gtagaacttg ccgtgctggg gattggcgtg ccacgggggc
     3901 atgcggccgt cgtcgatgac ttcgacgatc gtctcggccc acgccgctgc gtcttcgtag
     3961 ccagtcatgg aaaatggcgc cacttcgcct ggtcgatggc agcgcacgca attcttctgc
     4021 aaaatcccgg cgacgtgctt cgtgtaggtt atgtcccccg tcggcacacc gcgacgcacg
     4081 cggccgatgt aacagcccac gggctcttgt gagggcgggg cgacttcctt tcccgccagc
     4141 aggtcatcta atgcctgggc gacgaagctc ttggtcggct cattccgcga atagccgatg
     4201 ccatattggt cgtcgatccg cccccaatag cgcacgattc ctttgccatc caggacgaac
     4261 gcttccggcg tgcgcttcgc gccaaattga tccgcgacca cgccagccgg gtccgtgagc
     4321 agcggaaaac c
//
LOCUS       DASUGB010000293         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1005832, whole genome shotgun sequence.
ACCESSION   DASUGB010000293 DASUGB010000000
VERSION     DASUGB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005832"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFV87_05595"
     CDS             complement(<1..982)
                     /locus_tag="VFV87_05595"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5103261.1"
                     /translation="MTTLTQYTKTDIPGAYLLDPPSQKRDKSFQPQAHRPKSSPKTDS
                     GQKPSGHRSGRNNVNWPVVAWISLLHVGALAAPFFFSWEAVALTFVLHWVTGGIGICL
                     GFHRLLTHGSFQTYRPVKWLLCLVGGLAGEGSVIDWVANHRKHHAHSDQEGDPHSPHD
                     GPWWSHIFWLAHMLQAKAHEEHVKRWSPDLLKDPVVRFIGSMFLPAQFAFGLLLGTIG
                     YAYGGWFMAASFMAWGMFVRLVFVLHSTWFVNSASHMWGYKNYETTDDSRNNWWVALL
                     TYGEGWHNNHHAYPRMAPHGHKWWEIDLTFLTIRALQAVGLAWDVVDYKRR"
     gene            complement(1285..1968)
                     /locus_tag="VFV87_05600"
     CDS             complement(1285..1968)
                     /locus_tag="VFV87_05600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5103262.1"
                     /translation="MGESSFSQSSDAERAVAAPERRGLLLWPWIALLVAFALFLFYRF
                     NQPGAGDTDAVEDHPAIGSRIEQVQLAPLTGDGPPVRAVDLQGKVTLMNFWGPWCGPC
                     RIEFPHIQAIEQHYRNNREFQFLSISYQPPGSDEDIGPWTAEFLAEFDANFPTYHDPG
                     HEFLDQVRQIPGLVDFGFPTSFVLDRQGIIRGVWMGYAPGMEREIQQVIEKTLRETPA
                     KPVAGPAKP"
BASE COUNT          422 a          599 c          648 g          389 t
ORIGIN      
        1 cccgccgctt gtaatcgacc acgtcccagg ccaggccgac ggcctgcagg gcgcgaatcg
       61 tcaggaaggt gaggtcgatt tcccaccatt tgtggccgtg cggtgccatg cggggatagg
      121 cgtggtggtt gttgtgccag ccctcgccgt acgtcagcag ggcgacccac cagttgttgc
      181 ggctgtcgtc ggtcgtttcg tagttcttat agccccacat gtggctggcg gaattgacga
      241 accacgtgct gtgcagcaca aacaccagcc gcacgaacat gccccacgcc atgaacgagg
      301 cggccatgaa ccagccgccg taggcatagc cgatggtgcc gagcaggagg ccaaaggcga
      361 actgcgcggg caggaacatc gaaccgatga agcggacgac cgggtctttg agcaggtccg
      421 gcgaccagcg cttcacgtgc tcttcgtgag ctttggcctg caacatatgc gcgagccaga
      481 agatatggct ccaccagggg ccgtcgtgcg gggaatgcgg gtcgccttcc tggtcgctgt
      541 gcgcgtgatg tttgcgatga ttggcgaccc agtcgatcac cgagccctcg cccgccagcc
      601 cgccgaccag acacagcagc catttgaccg gccgataggt ctgaaagctg ccatgcgtga
      661 gcaggcgatg aaagcccagg caaatgccga tgccgccggt gacccaatgc agcacaaagg
      721 tgagagcgac cgcttcccag ctaaagaaaa atggtgctgc cagcgcgccg acatgcagca
      781 gcgagatcca ggcgacgacg ggccagttga cgttgttccg cccgctccga tggccggagg
      841 gcttttgtcc cgagtctgtt ttgggcgatg atttcgggcg gtgcgcttgc ggctgaaacg
      901 acttgtcgcg cttctggctg ggcgggtcga gcagataagc gccgggaatg tccgtcttgg
      961 tgtactgggt cagggtggtc atccgatgag ctcaacggtt cctgcgagga agttcgggtg
     1021 aatcgccttg cgcgggcgaa cttcatgacc gatttcgcag agaatcttaa ggcgtatcgg
     1081 acggattgct gtcttggcgg cgcgcgggga ttgtaatagt tgtgtaaggc cgcggacacg
     1141 agacgacgga agctgcctaa agcctttata taatacaact tacagccata cggcaggcgg
     1201 cctcgctgag ctgaatttga tccaagaaag acggtgggag aattaatttg cctcttgcgg
     1261 aagggcggtc gccgcaccct taaatcatgg ttttgccggt cctgctaccg gcttggctgg
     1321 cgtctcacgg agcgttttct cgatgacctg ctggatctcc cgctccatgc ccggggcata
     1381 acccatccaa accccgcgaa taatcccctg gcgatcgaga acaaacgagg ttggaaaacc
     1441 gaaatccacc aacccaggaa tctggcgcac ctggtccaaa aactcgtgtc caggatcgtg
     1501 gtaggtcggg aaattagcgt caaactctgc caggaattca gccgtccacg ggccaatatc
     1561 ctcgtcgctt cccggcggct gataagaaat ggaaaggaac tgaaactctc gattgttgcg
     1621 gtagtgttgt tcgattgctt gaatgtgcgg gaactcaatc cggcatggac cacaccacgg
     1681 gccccagaaa ttcatcaggg tgactttccc ctgcaagtcg actgccctga ccggtgggcc
     1741 gtcgcccgtc agcggcgcca gttgcacttg ttcgatgcgc gatccaatcg ccgggtgatc
     1801 ctcaacggca tcggtatcac ccgccccagg ctgattgaag cggtagaaca gaaacagtgc
     1861 aaacgccacc aggagcgcga tccacggcca cagcagcagc ccgcggcgct ctggcgccgc
     1921 aaccgctcgc tcggcatcgg agctttgtga gaagctggac tcgcccatgt cgctgcctcg
     1981 gcgatttgga ccgacgagaa agatcgcccg ccgggtcctg tggaacgacg gcgggcgagg
     2041 agtagcgggg ggcggact
//
LOCUS       DASUGB010000294         7572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1421456, whole genome shotgun sequence.
ACCESSION   DASUGB010000294 DASUGB010000000
VERSION     DASUGB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421456"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFV87_05605"
     CDS             <1..227
                     /locus_tag="VFV87_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103263.1"
                     /translation="LSRIILHLHQDKELVARLPLETQKGKAGEVQCHFQLIPALAKGA
                     VLELVCPEPNMPNGVLYEIDLTPYLPADEP"
     gene            273..587
                     /locus_tag="VFV87_05610"
     CDS             273..587
                     /locus_tag="VFV87_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103264.1"
                     /translation="MRFRLRTLLTFAMVATAVLAYVGCPLLDSPAVAWHLEWDMHDLS
                     RKLAIVLVFASAALAFFALVAAFGSEERVPAEFWLGYFASAAFSALLWALTLPWIQAA
                     RE"
     gene            594..1001
                     /locus_tag="VFV87_05615"
     CDS             594..1001
                     /locus_tag="VFV87_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103265.1"
                     /translation="MLRYNLRTLLIVLALGPGVLFVVSLVWFAIMVHFTNYPPRVKEY
                     PDSSGQTGLRRAYESANRTRAELRDGLRDLDDSPWRVVGDPNLPAPGTLPPPSGAFAT
                     GGPFSAPPDAPPPTIPVELERRTPEVHSPFSQD"
     gene            1516..1728
                     /locus_tag="VFV87_05620"
     CDS             1516..1728
                     /locus_tag="VFV87_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103266.1"
                     /translation="MKRALTLAMCMILGAIAGYFAAPFLVVALYGYDHGGETEEVALW
                     TIPGGALVGLALHLYFRYVEALPNHR"
     gene            1725..1940
                     /locus_tag="VFV87_05625"
     CDS             1725..1940
                     /locus_tag="VFV87_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103267.1"
                     /translation="MTEPRNRRSFAPWLGLLLALIAYPSLMLMVPDNSDDVALALRLL
                     IAAIAIWLVVIGLRAAWICRKPPGDFK"
     gene            complement(1968..2459)
                     /locus_tag="VFV87_05630"
     CDS             complement(1968..2459)
                     /locus_tag="VFV87_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103268.1"
                     /translation="MNFVSRCLKIGRMRLAFQFRIWDALALLTLAALALALLTNANEF
                     LAHTVATSEVLLVIAAITFIPVSAGPTQRFLIAFVASAVAYQLFESNDLFAPFRVIVE
                     QVWLNYITTENPRPAGVYYGALSGKELAFFDIAHSLIGLALASTSGIVAKAIRSQKVA
                     VKE"
     gene            2487..2642
                     /locus_tag="VFV87_05635"
     CDS             2487..2642
                     /locus_tag="VFV87_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103269.1"
                     /translation="MRFRLRTLLTALAAAGTLAGCRDPRIDQIQRGAENVDRHAQQIE
                     RAADPQP"
     gene            2708..3544
                     /locus_tag="VFV87_05640"
     CDS             2708..3544
                     /locus_tag="VFV87_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103270.1"
                     /translation="MVPRRYRVLLWTAPLLLGTWLFSAFSNDDPQGFYQFIFVGLFWG
                     TMFGHTTLAAAWAAFGPGPLLVRLPLSLFWVAMLPVAVAINLSVNSGPGQMAVPIGIC
                     LLGQWLALQFPLWGLALWLRLRLQCRGELERNPGREALQFTIRQLVVVTAVVGVIFGV
                     GRVLLPVVAQNYAQITIFALLCAAAIVLTLPLVLAALLHRFAVPGVLLVLALIAAATA
                     WESPAMQAMDGTSSVPPELLAAINAGTALMILAVLAIVRMCGYSLVPQPRHMQKTVVT
                     GC"
     gene            complement(3541..4938)
                     /locus_tag="VFV87_05645"
     CDS             complement(3541..4938)
                     /locus_tag="VFV87_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103271.1"
                     /translation="MLAIHDARPSGLTRRQVLAAGAMGGGLSLAGLLRAESAAGIGAS
                     KKAVINVHLDGGPPHLDMIDLKPDAPSEIRGEFSPIATRVPGIQICEHLPRLAAAADK
                     LVFIRSLVGAAGVHDAFQCQSGFDAKNLANVGGRPAMGCVVGKLRGSAADAAPSFVDL
                     MQGRPHVRNSARPGFLGPAFQPFRPDISALFERPLEPGMVGELSRRGDNHATSLALNA
                     ELPVERLGDRTNLLAGLDRLRKELDRSGMMEAQDSFTQQAVGILTSGKFAAAMDLSQE
                     DPRVLERYTLASDGEPPSTTSDRPDATKKFLLARRLVEAGVRCVSISISDFDTHSSNF
                     PRLKRLLPIVDHGLATLIADLDERGMLDDVSIVAWGEFGRTPTVDRKNGGRHHWPAVG
                     MALLAGGGIRAGQVIGATDRYAAAAISRPVHYQDVFATLYHNLGINARATTVTDPSGR
                     PQYLLDRGEVLPEVV"
     gene            5120..6961
                     /locus_tag="VFV87_05650"
     CDS             5120..6961
                     /locus_tag="VFV87_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103272.1"
                     /translation="MARVACMFLMAVASLLAIPPAARAQLGTEAPIELPAELTKPNLS
                     HAVTVDDALDIGPDWTYEPDEPQGEVQPLAKPVSFSLPYSTDAIAFSTARAAIAGVLQ
                     DVTETGGGKSIQRWNLYRYKLTTGQQSGPLKVYARTFESRTPGKVGNDRERLSIRAGA
                     LLTVGPDGKLAVVRTNDAGRLDVWSLADGGRHLVAWQPYGHESEFNRHVSWAAGLDDG
                     TVLTLNHQGRLIRWKLPECIALYRRELGRMSSFSSLPNLGVGLQPGLSVNHQHIAIWS
                     GKALLIIDAITGKTLAVLGGTEDPGWLRPTFHWDGTKLACTIAQDYVQILVIYDLRTG
                     AEVTRMPLPADAWPTPSTSNSRQGGFTWVGEPFGVVGSALLINLVERRPVWKFNEPPP
                     PLHAMPVVSTAGPDFWALTTAKSTQSLSSFQLPHADALQVAKEGPELFAAGPGSEISL
                     EVKADASEEVKSALEKAAMLRVQDVAMKLAKDARVKLLVEAEVRDVEEPGLVEGMRRS
                     LERAGIKDKVKIQVVTLRTSYLVDGKVAWSNSSDILPFGADVKEPKKSVPQQLLDGQW
                     QRVRDWARNLLQGIPGYVQDPAVLAALPSGPYPSEGTISDARSNPPR"
     gene            complement(6980..>7572)
                     /locus_tag="VFV87_05655"
     CDS             complement(6980..>7572)
                     /locus_tag="VFV87_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103273.1"
                     /translation="SDLVALGTGRMVGPPGNRREASEEYRPEKNLDLAEFFLSLGSRT
                     EFWEMHHSAGNHLNNHWCRVPPPDVWKQNGKPRYADVKFDRERYVADDGSRTVARAVH
                     LLPKADGQPSTVNDPSDIDAGYSGEIRFPWSGLGLPADRRRAGGSYRLAGLELQILAA
                     ALNGNGGEPRYYSSGELPSQMFHFSASRWPHYRLKP"
BASE COUNT         1287 a         2391 c         2435 g         1459 t
ORIGIN      
        1 atctctcgcg gattatcttg cacttgcacc aggacaagga actcgtcgcc cgcttgccgc
       61 tggaaacgca gaaagggaag gcgggcgaag tgcagtgcca cttccaacta atccccgcgc
      121 tcgccaaggg tgccgttctg gagctcgtct gccccgagcc caacatgccc aacggcgtgc
      181 tctacgaaat cgacctcacc ccctacctgc cggcggatga gccttgagcc acggcaaccc
      241 gtggcgcaat ctaactaacg ggcaaaactc ccatgcgatt ccgcctccgc acgctgctga
      301 cgttcgcgat ggtggcgacg gcagttctcg cttatgtggg ctgtccactg ctcgattccc
      361 ccgcggtagc gtggcacctc gaatgggaca tgcatgacct gtcgcgtaaa ctggcgattg
      421 tgctcgtgtt tgcgtctgcc gcattggcct ttttcgccct ggttgccgcc tttggctcgg
      481 aagagcgcgt gccggcggaa ttctggctcg gctacttcgc ctcagccgca ttttcagcgc
      541 tgctttgggc cttaaccctg ccgtggattc aagccgcccg agagtaatcg cccatgctcc
      601 gctacaacct ccgcaccctg ctgattgtgc tggccttggg gccgggggta ctatttgtgg
      661 tatcgctggt gtggttcgcg atcatggttc acttcaccaa ttacccgcct cgggtgaagg
      721 agtatcccga ttcgtcaggc cagacgggac tgcggcgagc ttacgaaagc gcgaaccgaa
      781 cccgcgcaga acttcgcgat ggactgcgag atctagacga ttcaccttgg cgggtcgttg
      841 gcgatccaaa cctgcccgcg cccggtacgc tgccgccgcc atcgggcgcc ttcgcgacgg
      901 gcggcccgtt cagtgcgcca ccggatgcac caccgccaac gattccagtc gagctagagc
      961 gtcgaacgcc ggaagttcat tcgccgttct ctcaggacta gccacgaatg ttccgattca
     1021 caatccgcga cgtgctgtgg ttgatggtgg tggtggcgct tgtaaccgca tggcttgtcc
     1081 agcgcaggga actcgaattc gccaaacgta cttccttggg cgcgaaagcc gatgtgcaga
     1141 gcctggtgac gcttttgcac gaaagcggtt atcgggtcat gtgggacgaa gaattgggaa
     1201 caataggtgc tcttgcggct gaccctagac tgccacgagc aggtgaacct aggtaccgcg
     1261 acgacgtacc cgtgcgacgt ccctacaggc gaccgttgac agactagaag cagttacaga
     1321 accctcgttt tcgcttgcga tgctgtcaat tcacccagtt tccagcactc tctgcaggcg
     1381 aatacgggta ttgaaactgc ttgtagaaca ctccccaact gcagccggcc tcaccgccgc
     1441 ggccctctgg ttcctgatgg tcgcatggct agcggtctgg ggggatcata ccctgctagg
     1501 tcggatgttc cggcgatgaa acgcgcgctc acactcgcga tgtgcatgat cctgggcgcg
     1561 atcgccgggt actttgccgc gccgtttctc gtggttgcct tgtacggcta tgaccacggc
     1621 ggcgaaaccg aggaagtggc gctgtggacg attcccggcg gagcgctggt gggtttggcg
     1681 ctgcacctct acttccgcta cgtagaagcg ctgccgaatc accgatgacg gaacctcgaa
     1741 accgccgcag tttcgcaccc tggctgggtc tgcttctggc gctcatcgcc tacccgagtt
     1801 tgatgctgat ggttcctgac aactcggatg atgtggctct tgcgctccgt ctcctcattg
     1861 cggcaattgc gatctggctg gtcgtgatcg gccttcgagc cgcgtggatt tgccgcaagc
     1921 cgccggggga tttcaagtag caaactcgcc ggcgctgaca acgaggttta ctccttgacg
     1981 gctacttttt gtgacctaat cgctttggcc acgatccccg acgtactggc cagcgcgagt
     2041 ccgatgaggg aatgagcgat gtcgaagaag gcaagttctt taccgctaag tgcgccgtag
     2101 taaactcccg caggccgtgg attctcggtc gtaatgtaat tcaaccacac ttgctccacg
     2161 atcacccgga acggcgcaaa caaatcgttc gattcaaaga gctgataggc gactgcgctc
     2221 gctacgaacg caatgagaaa tcgctgggtc ggacctgccg aaacggggat gaacgtaatc
     2281 gccgcgatga ccagaagcac ttcggacgtg gccacagtgt gggccaggaa ctcgttggcg
     2341 ttggtcagaa gagccaacgc tagtgccgcc aatgtcagga gcgccagcgc atcccaaatc
     2401 cggaattgga aagccaatcg cattctgcca attttaaggc accgcgaaac gaagttcatg
     2461 tagaatcggc ctcaaggttg ccttccatgc gtttccgcct ccgcacgctg ctgactgccc
     2521 tcgctgcggc tgggactttg gctggttgcc gtgatccgag aatcgatcaa atccagcgtg
     2581 gggccgaaaa cgtcgatcgg catgcccaac agatcgaacg agcggctgat ccgcagccgt
     2641 aggagctgcc catgttacga ttcacgacct gtgatgtgct ggcgctcgcg taggtggagc
     2701 acggaacgtg gtccctcgcc gttaccgagt tctattgtgg acggcgccgc tgctcctggg
     2761 gacgtggctc ttttccgcat tctcgaacga cgatccccag ggcttttacc agttcatctt
     2821 cgtcggcttg ttctggggga cgatgttcgg gcacaccacg ttggcagccg cgtgggcggc
     2881 gtttggcccg ggtccgctat tggtgcggct gccgctgtcg ctgttttggg tggcgatgct
     2941 gccggtggcc gtggccatca atctgtccgt caattccggt ccgggacaaa tggcggtccc
     3001 gatcggcatc tgtttgctgg ggcaatggct cgcgcttcag tttccgttgt gggggctggc
     3061 gctttggctg cgcctgcggc tgcaatgccg gggcgaactt gagcggaatc ctggccggga
     3121 agcgttgcaa ttcacgattc gccagctcgt ggttgtgacg gcggtcgtcg gagtgatctt
     3181 tggcgtgggc cgtgtgctct tgccggttgt tgctcaaaat tacgcccaaa tcacgatctt
     3241 cgccctgctg tgtgcggcgg cgatcgtgct gacgctgccg ctggttctgg cggcgctgct
     3301 ccaccgcttc gcagtgcccg gcgtgctgct ggtgctggcg ctgatcgcgg cggcgaccgc
     3361 ttgggaatcg cccgcgatgc aagcgatgga cggcacttcc tcggtgccac cggaattgct
     3421 ggcggccatc aacgccggca cggccctgat gatcctggcc gtcctggcca tcgttcgaat
     3481 gtgcggctac agcctcgtcc cgcagccgcg gcatatgcaa aagaccgttg taaccggctg
     3541 ctagaccacc tcgggcagca cttcgccgcg gtcgaggagg tattggggcc ggccgctggg
     3601 gtcggtgacg gtcgtcgcgc gggcgttgat ccccaggttg tgatagagcg tggcgaagac
     3661 gtcctgatag tggaccggcc tgctgatggc ggcggcggcg tagcggtcgg tcgcgccgat
     3721 gacctgcccc gcgcggatgc cgccgccggc caaaagcgcc attccgaccg ccggccagtg
     3781 gtgccggccg ccatttttgc gatcgaccgt cggcgtgcgg ccgaattcgc cccaggcgac
     3841 gatcgacacg tcgtccagca tcccgcgctc gtccagatcg gcgatcagcg tcgccagccc
     3901 gtgatcgacg atcggcagca gacgtttcag ccgcgggaag ttgctcgagt gcgtatcgaa
     3961 atccgatatc gagatgctca cgcaccgcac gcccgcttcg accaggcggc gggcgagcag
     4021 gaacttcttc gtggcatcgg gccggtcgct cgtcgtcgaa gggggctcgc cgtccgatgc
     4081 gagcgtgtac cgctccagca cgcgcgggtc ttcctgcgac aggtccatcg ccgcggcgaa
     4141 cttgccgctg gtgagaatgc cgaccgcttg ctgtgtgaac gagtcctggg cctccatcat
     4201 cccgctgcga tcaagctctt tccgcagccg atcgagcccc gccagcaggt tcgtccggtc
     4261 gcccagccgc tcgaccggca gctcggcatt gagggccagc gaagtcgcgt gattgtctcc
     4321 gcggcggctg agctcgccga ccatcccggg ctccagcggg cgctcgaaga gggccgaaat
     4381 atcgggccgg aacggctgaa acgcgggccc gaggaagccg gggcgggcgc tattccgcac
     4441 atgcggccgg ccctgcatca gatcgacaaa cgagggcgcg gcatccgccg ccgagccgcg
     4501 gagcttgccc acgacgcagc ccatcgccgg ccggccgccg acgttcgcca ggtttttcgc
     4561 atcaaagccg ctctggcact ggaaggcgtc gtgaacgccc gccgcaccga ccagcgagcg
     4621 gatgaagacc agcttgtccg ccgcagccgc cagccgcggc agatgctcgc agatctgaat
     4681 gccggggacg cgcgtggcga tcgggctgaa ctcgccgcgg atttcgctcg gcgcgtcggg
     4741 cttgaggtcg atcatgtcca gatgcggcgg cccgccatcc agatgcacgt tgatcaccgc
     4801 cttcttggac gcgccgatcc ccgccgccga ctccgcccgc agcagccccg ccagtgacag
     4861 cccgccgccc atcgccccgg ccgccagcac ctgccgccgc gtcaacccac tcggtcgtgc
     4921 gtcatgaatc gcaagcatcc gccggccctc cgcacccgaa ttcgtcccgg ccagtgtagc
     4981 ggatcgctcg cccaaagaga aacaattatt gacgcgtcga tttgatttcc ttggcctggc
     5041 tgatggcggc gcatcgaagc tgttgctgcg cggcttttgc tggttacaat gcgggcctca
     5101 tttcccttgg aggtgtgaca tggcccgcgt tgcctgcatg ttcttgatgg ccgttgcatc
     5161 gctcctggcg attccgcccg cggcacgggc ccagcttggc accgaggcac ccatcgaatt
     5221 gcccgcggag cttaccaagc ccaacctttc gcatgccgtg accgtggacg atgccctcga
     5281 cattggaccg gattggacct acgagcccga cgagccgcag ggcgaggtcc agccgctcgc
     5341 caagcccgtc tcgtttagcc tgccctattc caccgatgcg attgcttttt ccacggcgcg
     5401 cgcggcgata gccggcgtat tgcaggacgt gaccgaaacg ggcggcggga agtcgatcca
     5461 gcgctggaac ctgtaccgct acaagctgac gacgggccag caaagcggcc ccctcaaggt
     5521 gtacgcgcgg accttcgagt cccgcacgcc gggaaaggtc ggcaacgacc gcgagcggct
     5581 ttcgattcgc gccggagcgc tgctcacggt gggaccggat gggaagctgg cggtcgtgcg
     5641 gacgaacgat gccggccggc tcgacgtgtg gtcactggcg gatggcggca ggcaccttgt
     5701 cgcctggcaa ccctatggcc acgaatccga gttcaatcgc cacgttagtt gggcggccgg
     5761 gctcgatgat gggacggtcc tgacgctcaa ccaccagggc cgcctgatcc gctggaagct
     5821 gcccgagtgc atcgcccttt accggcggga actcggccgg atgtcgagct tcagcagcct
     5881 gccgaacctg ggcgtcggcc tacagccggg actgagcgtg aaccatcagc acatcgcgat
     5941 ctggtccggc aaggcgctct tgattatcga cgccatcacc ggcaagacgc tcgcggtcct
     6001 gggaggcacc gaggatcccg gctggctacg gcccacgttt cattgggacg gcacgaagct
     6061 ggcctgcacc atcgcccagg attacgtgca gatcctcgtc atctacgacc tcagaacggg
     6121 cgcggaggtg acgcggatgc cgctgcccgc ggatgcctgg cccacgccga gcacgtcgaa
     6181 ctcgcggcag ggcggattca cttgggtcgg cgagcccttt ggcgtcgtcg gttcggcgct
     6241 gctgatcaat ttggtggagc ggcggccggt ctggaagttc aacgagccgc ctccgcccct
     6301 gcacgccatg ccggtcgtct ccacggcagg tcccgacttt tgggccctta ccacggccaa
     6361 gagcacccag tccctcagct cgttccagct tccccacgcc gatgcgctgc aggtcgccaa
     6421 ggaaggtccg gaactgttcg ccgccggacc tggatcggag atttcgctgg aagtgaaggc
     6481 cgacgcttct gaagaagtga agtctgcttt ggaaaaggca gccatgctcc gcgtgcagga
     6541 cgtggcaatg aagctggcca aggacgcgcg ggtgaaattg ctggtcgaag cggaagttcg
     6601 cgatgttgag gagcctgggc tcgtcgaagg aatgcggcgc tccctggagc gcgccggcat
     6661 caaagacaag gtcaaaatcc aagtcgtcac gctgcgcacg agttaccttg tggatggcaa
     6721 ggtagcctgg agcaattcga gcgacatctt gccttttggg gcagatgtga aagagccgaa
     6781 aaaatcggtt ccccagcagt tgctggacgg ccaatggcaa cgcgtccggg actgggcccg
     6841 gaatctatta caagggattc caggctacgt ccaggacccg gcggttctgg cggcgctgcc
     6901 ctccgggccg tatcccagcg aggggacaat ctcagacgcc cgctccaatc cgcctcgctg
     6961 attcgcgggc agcacgcgct catggcttga gacgataatg cggccagcgg ctggcggaga
     7021 aatggaacat ctggctgggt agctcgccgc tggaatagta gcggggttcg ccgccgttgc
     7081 cgttgagggc ggcggcgagg atttgcagtt cgaggcccgc gaggcggtag ctgccgcctg
     7141 cgcggcggcg gtcggcgggc aggccgaggc ctgaccaggg gaagcggatt tcgccgctgt
     7201 agccggcatc gatgtcggac ggatcgttaa cggtcgaagg ctggccgtcg gctttgggaa
     7261 gcagatggac ggcgcgggcg acggtgcggc tgccgtcgtc ggcgacgtac cgctcgcggt
     7321 cgaacttcac gtcggcatat cgcggcttgc cgttctgctt ccatacgtcc ggcggcggca
     7381 cgcggcacca gtgattgttg aggtggttgc cggcggagtg gtgcatttcc cagaactcgg
     7441 tccggctgcc gagtgacagg aagaactcgg ccaggtcgag gttcttctcg ggccggtatt
     7501 cctcgctcgc ttcgcggcgg ttgcctggag ggccaaccat gcggcccgtg ccgagcgcca
     7561 ccaggtcgct at
//
LOCUS       DASUGB010000295         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_867367, whole genome shotgun sequence.
ACCESSION   DASUGB010000295 DASUGB010000000
VERSION     DASUGB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867367"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFV87_05660"
     CDS             complement(<1..583)
                     /locus_tag="VFV87_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5103274.1"
                     /translation="MENDLLRWFRATLPPHKQLVLGAGDDAAVIQLTGGRQLVATTDM
                     LMDGVDFRVGQHDPAAIGHKALAVNLSDLAAMAARPVAALVSLCLPRTGGESLAKQLF
                     EGILPTAAQYGLAIAGGDTNSWDGPLAISVTALGEVTPGREWRRSGARPGDSIVVTGS
                     FGGSILGSHLTFDPRVEEALWLAENLTVHAAIDV"
     gene            complement(648..2018)
                     /locus_tag="VFV87_05665"
     CDS             complement(648..2018)
                     /locus_tag="VFV87_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5103275.1"
                     /translation="MPEHTVCVPARRLLLTGLAVASALLLAAATPRPSHADASELRNS
                     PIVKAVHAAAPAVVNIHGRKTVRAENASYGAGETVRQVNGMGTGIVIDPRGYIITNFH
                     VVEGVTNIQVTLFDERAAVARLVANDPKTDLAIIKIDSKEKLPVVPFGTSCDLMCGEP
                     VIAVGNAFGYEHTVTRGIISALHRSVQVSDDQKYSDLIQTDASINPGNSGGPLMNIDG
                     QVIGINVAVRVGAQGIGFAIPIDEALEVAARLMSIERLDQATHGIVGKTVCEPATRSF
                     LVSGVKSDSPAARAGLKAGDTITRIGERTVERALDVELALLGRKAEEDVAIEFTRDGQ
                     LVSDKIAVARVTRSQLKPALADRVWTILGLRLAPMDETAFRDLGTRYRGGLSVIAVRP
                     EGPAAQQGIKRGDVLVGMHVWETISLENIAYILDREDLAKFDPLTFYIVRGSDTLYGH
                     LRVAGR"
     gene            complement(2247..2729)
                     /locus_tag="VFV87_05670"
     CDS             complement(2247..2729)
                     /locus_tag="VFV87_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103276.1"
                     /translation="MRTVQLATLACLLVAGGCCLPCLSGRYCDQPPAADQPQPATKQP
                     SALASQSPMGAEPNHLAHPDKIGHAGLHGAGMRHAGLHDRFHALHGLAGGMATPPGEL
                     IPLPKFHPVPVHPVFEPQLDYLIPQPLELQPESASTPPAADGLPTPATPPAKALPGAA
                     "
     gene            complement(2817..>3250)
                     /locus_tag="VFV87_05675"
     CDS             complement(2817..>3250)
                     /locus_tag="VFV87_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103277.1"
                     /translation="AEYHEFSSVLASLADAIDAPAAGNADWLDACRDQEAAEAIDRSL
                     ARGRTIELFNEEHTEEESFKGVMAVGGCLLLLGALGVVLLATIVEGLQLPLRDWAAWQ
                     YWPVYLLAPIVVFLLLQVLQVAIRKHPPSLRQLVDGDDQAA"
BASE COUNT          476 a         1045 c         1209 g          520 t
ORIGIN      
        1 tgacatcgat ggcggcgtgg accgtcaggt tctcggccag ccacaaggcc tcttcgaccc
       61 gcggatcgaa cgtcaagtgg ctgccgagga tgctgccgcc gaagctgccg gtgaccacga
      121 tcgaatcgcc cggacgggcc ccgctgcggc gccactcgcg gccgggagtc acttcgccga
      181 gggccgtgac gctgatcgcc agcggcccgt cccagctgtt cgtgtcgccg ccggcgattg
      241 ccaggccata ctgggcggcc gtcggcagga tgccctcgaa gagctgtttg gccagcgact
      301 cgccgccggt tcgcggcagg cagagcgaaa cgagtgcggc cacggggcgg gccgccatcg
      361 ccgccaggtc gctcaggttc acagccagcg ccttgtggcc gattgcagcc gggtcgtgct
      421 ggccgacgcg gaaatcgacg ccgtccatca gcatgtcggt cgtggcgaca agctgcctgc
      481 cgcccgtgag ctggatgacc gccgcatcgt cacccgcgcc gagcaccagc tgtttgtgcg
      541 gtggcagcgt cgcgcggaac cagcggagca ggtcgttttc catcctgcgc attatcagcg
      601 caaagcagcg gtcaagcgag gcgcaggtcc cgcagggcct gacggaccta ccttccggca
      661 acccgcaggt gcccatacag cgtgtcgctg ccgcgcacga tgtagaacgt gagcgggtcg
      721 aacttggcca ggtcttcgcg gtccaagatg tacgcgatat tctccagcga gatggtctcc
      781 cagacgtgca tgccgacgag tacgtcgccc cgtttgatgc cttgctgggc ggcgggacct
      841 tccggacgga ccgcgatgac ggagaggccg ccgcgatagc gcgtgcccag gtcgcggaac
      901 gccgtttcgt ccatcggcgc cagccgcagg ccgaggatcg tccagacgcg gtcggccagt
      961 gcgggcttga gctgcgagcg ggtgacgcgg gcgacggcga tcttgtcgct gacgagctgg
     1021 ccgtcgcggg tgaattcgat ggccacgtcc tcttcggcct tgcggccgag cagggccagc
     1081 tcgacgtcga gcgcccgctc gacggtgcgc tcgccgatcc tggtgatggt gtcgcccgct
     1141 ttgaggccgg cgcgggccgc ggggctgtcg ctcttgacgc cgctgaccag gaagctgcgc
     1201 gtggccggct cgcagaccgt cttgccgacg atgccgtgcg tggcctggtc gagccgctcg
     1261 atgctcatca ggcgggccgc gacctccagg gcctcgtcga tcgggatggc gaagccgatg
     1321 ccctgagcgc cgactcgcac ggcgacgttg atgccgatga cctggccgtc gatgttcatc
     1381 agcggcccgc cggagttgcc ggggttgatg ctggcgtcgg tctgaatcag gtcgctgtac
     1441 ttctggtcgt cgctcacctg cacgctgcgg tgcagggcgc tgatgatgcc acgggtcacg
     1501 gtgtgctcgt agccgaaggc gttgcccacc gcgatcaccg gctcgccgca catcaggtcg
     1561 cacgacgtgc cgaaggggac gaccggcagc ttttctttcg agtcgatctt gatgatcgcg
     1621 aggtcggtct tggggtcgtt ggcgacgagg cgggcgacag cggcccgctc gtcgaacagc
     1681 gtgacctgga tgttggtcac cccttcgacg acgtggaagt tggtgatgat gtagccgcgg
     1741 ggatcgatga cgatgcccgt gcccatgccg ttgacctggc ggacggtttc gcccgcgccg
     1801 taggaggcgt tttcggcccg cacggtcttg cggccgtgga tgttgacgac ggcgggcgcg
     1861 gccgcgtgca ccgccttgac gatgggcgag ttgcgcagct cgctggcgtc ggcatggctg
     1921 gggcggggcg tggcagcggc cagcagcaag gcggaggcga cggccaggcc cgtcagcagg
     1981 aggcgacgcg cgggcacgca gacggtgtgt tcgggcatga ggtgctcgtc aggcctgggt
     2041 cgtcttgcca gtgcgccgcc gtcgccgcgg gtgctggcgc gcggggggcg caaggtagat
     2101 gggtccgatg gatagatcgg ccccgtttgg cggaacgctt cagaggggga gcgcgggccg
     2161 gaagactccg cccaacttgc caggcgcagg cccgcaagtg gaaatctccc aggcgagcgg
     2221 ctgctagcgg gcagcgggta cagaggctag gcggcgccgg gaagcgcttt ggccggcggc
     2281 gtggccgggg tcggcagacc atcggccgcg ggtggagtgc tcgccgactc cggctgcagc
     2341 tcaagcggct gcgggatgag gtagtccagc tgcggctcga aaaccggatg cacgggcacc
     2401 ggatggaact ttggcagcgg gatcagctcg ccgggcgggg ttgccatgcc gccagccagg
     2461 ccgtgcaggg cgtggaatcg gtcgtgcagg ccggcgtggc gcatgccggc accgtgcaag
     2521 ccagcgtggc cgattttgtc tgggtgagca aggtggttcg gctcggcgcc catcggcgac
     2581 tggctggcta gagcggacgg ctgcttcgtt gcaggctgcg gctgatcggc agccggcggc
     2641 tggtcgcagt agcggcccga gaggcacggc agacagcagc cgcctgccac cagcaagcag
     2701 gcgagggtcg cgagttgaac agtgcgcatg accggccggt ccttgttgcg gcgtggtatc
     2761 ggcctgccgg ggcgcagact ttagccgacc ggcagggatt ggcggaccgg cccgacttat
     2821 gccgcctggt cgtcgccgtc gaccagctgg cggaggctgg gcgggtgctt ccttatcgcc
     2881 acttgcagca cctgcaagag caggaacacc acgatcggcg ccagcagata gaccggccag
     2941 tactgccagg cggcccaatc gcgcaggggc agctgcagcc cctcgacgat cgtggctagc
     3001 agcacgacgc ccagcgctcc cagcaagagg aggcagccgc cgaccgccat cacccccttg
     3061 aacgactcct cttcggtgtg ctcctcgttg aagagctcga tcgtgcggcc gcgggccagg
     3121 ctgcgatcga tcgcctcggc cgcctcctga tcgcggcagg cgtcgagcca gtcggcgttg
     3181 ccggccgcgg gggcgtcgat cgcatcggcg aggcttgcca gcaccgacga gaactcgtgg
     3241 tactcggcca
//
LOCUS       DASUGB010000296         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1700379, whole genome shotgun sequence.
ACCESSION   DASUGB010000296 DASUGB010000000
VERSION     DASUGB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700379"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VFV87_05680"
     CDS             complement(<1..1233)
                     /locus_tag="VFV87_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX5103278.1"
                     /translation="MPSRDPALARQFAVDVVRRLHLAGHQALWAGGCVRDQLLGLVPK
                     DYDVATNATPEQIREIFGKRRTLAIGAAFGVITALGPEGAGQIDIVTFRQDAPYSDGR
                     HPDSVSFADAEQDAQRRDFTINGLFYDPLHSRVIDYVGGQEDLRQGLVRAIGDPLARI
                     AEDKLRMLRAVRFAARFDFVIDLPTLAAVQQQAHELVIVSAERIAAELRLIFTHRSRA
                     RGVELLQEAQLLEIVLPEARLLLEDTAAWERTLRILAALVQPTFSMALAALLREIPAL
                     DHTIHNLPRLVFQRWKLATEEYEGVAKLLAEEPLILGASRLPWPRLQRMLIAPRVEEL
                     LGYCEAVARGAGISSAEREVECCRTKLAVPPNELNPPPLITGDDLKAAGIPPGPEYRE
                     LLEAVRDAQLIRQIATHQQ"
     gene            1333..1671
                     /locus_tag="VFV87_05685"
     CDS             1333..1671
                     /locus_tag="VFV87_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5103279.1"
                     /translation="MPDLPLEIDVLSVKQMQVRGEKFTLLDCREPDEVATAKIAGSMH
                     IPMREIPSRLAELEAAKDGLIVVHCHHGGRSLRVTHFLRQQGFAGVQNLSGGIDAWSQ
                     QIDPRVPRYE"
     gene            complement(1753..2406)
                     /locus_tag="VFV87_05690"
     CDS             complement(1753..2406)
                     /locus_tag="VFV87_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5103280.1"
                     /translation="MNERDIIPQTAEHVRSLLAGDATGHDWFHIERVRKTALAIAREE
                     GADLFVVELAALLHDVADWKFAEGDHTASPRAARRWLEALEVNPEIVGHIEEIIAGLS
                     FKGAGVPTPMRTLEGQIVQDADRLDALGAIGIARTFAYGGHKGQPLHDPSLPAQSHAS
                     FEAYKNNVGTSINHFYEKLLLLKDRMNTATGRRLAEERHTYTEQFLEQFFAEWDGKR"
BASE COUNT          465 a          802 c          785 g          444 t
ORIGIN      
        1 ttgttgatgc gtcgcgattt gccggatgag ctgagcatcg cgtacggcct cgagcagctc
       61 gcggtattcc ggccccggcg gaatgcccgc ggccttcagg tcgtcgccgg tgatcagcgg
      121 cggcggatta agctcgtttg gcggcacggc gagcttggtt cggcagcatt cgacttcccg
      181 ctcggcagac gagatgcctg ccccacgggc tactgcttca caatagccga gcaactcttc
      241 gactcgcggc gcgatgagca tccgctgcag ccgcggccag ggtaggcggc ttgctcccaa
      301 gatcagcggc tcttcggcca ggagtttggc cacgccctcg tattcctccg ttgccagctt
      361 ccaccgctgg aacactagcc gtggcaggtt gtggatcgtg tgatccagcg ccggaatctc
      421 gcgcagcagc gccgccaaag ccatggaaaa cgtcggctgc acgagggccg ccaggatccg
      481 cagcgtccgc tcccacgccg cggtgtcctc cagcagcagg cgagcttctg gcagcacgat
      541 ctccagaagc tgggcttcct gcagaagctc cacgccccgc gctcggctcc ggtgcgtgaa
      601 gatcagccgc aattcggcgg cgatgcgttc ggcgctaacg attaccaatt cgtgggcctg
      661 ctgctggacg gcagcgagcg tcggcaggtc gatcacgaaa tcgaaccggg cggcgaaccg
      721 cacggcccgc agcatccgca gcttgtcctc cgcgatgcga gcgagcggat cgccaatcgc
      781 ccgcacgagg ccctgcctca ggtcctcctg gccgccgaca taatcgatca cgcgcgagtg
      841 cagcggatcg taaaacaggc cgttgatcgt gaaatcgcgg cgctgggcgt cctgctcggc
      901 gtcggcgaag ctgaccgaat cgggatggcg gccgtcgctg tacggggcat cctggcgaaa
      961 agttacaatg tcgatttgcc ccgccccttc cggccccagt gccgtgatca cgccgaaggc
     1021 cgccccgatc gccaaggtcc gccgtttgcc aaagatttcg cgaatctgct cgggcgtggc
     1081 gttggtcgcc acgtcgtagt ccttcggcac gaggcccaga agctgatcgc gcacgcaacc
     1141 accggcccac agcgcctgat gccccgccag gtggagccgg cggacgacgt ccacggcaaa
     1201 ctggcgggcg agcgcggggt cgcgggaggg catcggatca gggggacagg ggacaggatg
     1261 atgactacac agtactctat cgttacctag cgcagcttgg cttccgattt ctgacctccg
     1321 acctctgaac ccatgcccga ccttcctctc gaaatcgacg ttctgtcagt caaacagatg
     1381 caagtccgcg gcgagaagtt cacactcctc gactgccgcg agccagacga agtggcgacc
     1441 gccaagatcg ccggctcaat gcatattcca atgcgcgaaa tccccagccg cctggcggaa
     1501 ttggaagcgg ccaaagacgg gctgattgtc gtccattgtc atcacggggg gcgcagcttg
     1561 cgggtgacgc atttccttcg tcagcaaggg tttgcgggcg tccagaacct gtccggcggc
     1621 atcgacgcct ggagccagca gattgatccg agagtccccc ggtatgagta gttctctgct
     1681 cgcggtcatg accatcagca gaccggaagc agcatcgtca agtcatgaaa tggacgcgcc
     1741 gccgtgcggc agttaccgct ttccatccca ttcagcaaag aactgctcga ggaactgttc
     1801 agtataggtg tgccgctcct cggccagccg acggccagta gccgtattca tgcggtcctt
     1861 caggagcagc agcttctcgt agaaatggtt gatcgaagtg ccgacgttgt tcttataagc
     1921 ctcgaacgag gcgtgcgact gggccggaag gcttggatcg tggagcggtt ggcccttgtg
     1981 gccgccgtag gcgaacgtgc gggcgatgcc gatggccccg agggcatcga gccgatcggc
     2041 atcctggacg atctgccctt cgagcgtgcg catcggcgtg gggacgccgg cgcctttgaa
     2101 cgagagtccg gcaatgatct cctcgatgtg accaacaatt tcggggttaa cttctagcgc
     2161 ttccaaccag cggcgagcag cccgcgggct ggccgtgtga tcgccctcgg cgaacttcca
     2221 atctgccacg tcgtgcagca aagccgccag ctcgacgacg aagaggtccg cgccttcctc
     2281 gcgggcgatc gccagcgccg tcttgcggac ccgctcgatg tgaaaccagt cgtggccggt
     2341 cgcatcgccc gccaacaggc tccgcacgtg ctcggccgtt tgcggtatga tgtcgcgttc
     2401 gttcaaggga aatctcgctg gataagacac gcaccaggga actctcacca tggccaaaaa
     2461 gaagcgcaaa gacaagccga ccagaagcga taagcg
//
LOCUS       DASUGB010000297         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_867769, whole genome shotgun sequence.
ACCESSION   DASUGB010000297 DASUGB010000000
VERSION     DASUGB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867769"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>2249
                     /locus_tag="VFV87_05695"
     CDS             87..>2249
                     /locus_tag="VFV87_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103281.1"
                     /translation="MSDVALPPKPLAQQTRGVVARLHALRTQIAAWFWVDGLVRVLWM
                     ALALLGIDLAIDWFFRLDKPQRIVMLFLIAGVIGWRIYRRLIRPLSASMSDEALALSV
                     EAGNKQLGQSMISALQLAQMEDAESRGMSPALVAQTVRHGTAAAEKVSFSNILDGREF
                     RLNAVLLVLALGVFGLIGYGVATTETVSIWFNRNILLGYKTWPQKTYLVIERVGEDGT
                     VVFPRGEDWTQTVAVRDDSEVIPDAVYLDFRRARGRAPVAMKKTSERLFETTFASVIE
                     PFEFRARGGDAVTEWVRVELVEQPAVESLKLVVTPPKYASNKPEELPPGKGPYYVLKG
                     SSLAIEGTANKPLVQAELLVEGKRLPLKIADKLRFSGQISAADLAPGQYTIELKDELG
                     LTSRRPTTFGLRTRIDREPRVRVKLIGVSGMVVPQARVPFTCRVTDDFGLTSADVAYR
                     WKGDDLTQPDGEGKLTFEQVKDLLGKADLSFDDVIELEPLQIPTGTGLSFRFESADND
                     DISGPNIGRSSEFLLRIVTEEELRTDLLRREKEQRQEFERLLKNQEDLVTDSRALEAG
                     TKGQAGLAPPQKDQLIQYHKRQKLVGQNTGAIAERMFSIVIEVQNNRLEEDGGRLQTR
                     LLKEIIEPMRVVVEEMIPQAQSGLESVRRLADDAAARDAALAETIAQQTEIAAKMKEI
                     LEHMVKSEGFQEAVNLLYEIQKAQTDVHEQTNKERQERI"
BASE COUNT          435 a          716 c          749 g          349 t
ORIGIN      
        1 ttccaaacga ggggtgctcg gggggtgcgg tctcgtaccg cccgcccgcc gtgtgagtca
       61 tttaccgctg agatgaagtg agagaaatgt ccgacgttgc cctcccgccc aagccgctcg
      121 cccagcaaac ccgcggcgtc gtcgcccggc tgcacgccct gcgaacgcag atcgccgcct
      181 ggttctgggt cgatggtctg gtgcgcgtgc tgtggatggc gctggccctg ctcggcatcg
      241 acctggcgat cgactggttc ttccgcctgg ataagccgca gcgaatcgtg atgctgttct
      301 tgatcgcggg cgtcatcggc tggcggattt atcgccggct gattcggccg ctgtcggcgt
      361 cgatgagcga cgaagcgctg gccctctcgg tcgaggcggg gaacaagcag ctcggccaaa
      421 gcatgatcag tgccctgcag cttgcccaga tggaagacgc cgaaagccgc ggcatgtcgc
      481 cggcactcgt cgcccagacc gtccgccacg gcaccgccgc agccgagaag gtgagcttca
      541 gcaatatcct cgacggccgc gagttccgcc tcaacgccgt gctgctggtg cttgccctgg
      601 gcgtgtttgg cctcatcggc tacggcgtgg cgacaaccga gacagtctcg atctggttca
      661 accgcaacat cctgctgggt tacaagacct ggccgcagaa gacgtacctg gtgatcgagc
      721 gcgtcggcga ggacggaacc gtcgtctttc cgcgcggcga ggattggacg cagaccgtcg
      781 ccgtgcgtga cgacagcgaa gtgattcccg atgcggtgta tctcgatttc cgccgcgccc
      841 gcggccgcgc gcccgtggcg atgaagaaga ccagcgagcg gctgttcgag accacgttcg
      901 ccagcgtgat cgagccgttc gagttccgcg cccgcggtgg cgacgccgtc accgaatggg
      961 tccgcgtcga gctcgtcgag cagccggcgg tcgagtcgct gaagctggtg gtcacgccgc
     1021 cgaaatatgc ctccaacaag ccggaggaac tgccgccggg caaagggccg tactacgtgc
     1081 tcaaggggag ctcgctggcg atcgaaggca cggccaacaa gccgctcgtg caggcagaat
     1141 tgctcgttga aggcaaacgc ctgccgctga aaatcgcgga caagttgaga ttcagcgggc
     1201 agattagcgc tgccgatctg gcgccgggcc aatacacgat cgagttgaag gatgagctgg
     1261 ggctcaccag ccgccggccc accacattcg gcctccggac gcgaatcgac cgcgagccgc
     1321 gggtccgcgt gaaactgatc ggcgtcagcg gcatggtcgt gccccaggcc cgcgtcccgt
     1381 tcacctgccg cgtgacggac gatttcggcc tcaccagcgc cgacgtcgcc taccgctgga
     1441 agggagacga cctcacgcag cccgacggcg aggggaagct cacgttcgag caggtgaaag
     1501 acctgctggg caaggcggac ctctccttcg acgacgtcat cgagctggag ccgctgcaga
     1561 ttccgaccgg caccggtctt tcgttccgct ttgagtcagc cgacaacgac gacatcagcg
     1621 ggccgaacat cggccgctcg tccgagttct tgctgcggat cgtcaccgag gaagaattgc
     1681 ggaccgacct tttgcgccgc gaaaaggagc agcggcagga gttcgagcgg ctcttgaaga
     1741 atcaggaaga cctcgtcacc gacagccggg ccctggaagc gggcaccaag gggcaggcgg
     1801 gcctcgcccc gccgcagaag gaccagctca tccagtatca caagcggcaa aagctcgtcg
     1861 gccagaacac cggcgcgatc gccgagcgga tgttctcgat cgtgatcgag gtacagaaca
     1921 accggctgga ggaagatggc ggccggctgc agacgcggct gctgaaggag atcatcgagc
     1981 cgatgcgcgt ggtggtggag gaaatgattc cgcaggccca gagcgggctc gaatcggtac
     2041 gccgactggc agacgatgcc gcggcgcgcg acgccgcgct ggccgagacg atcgcccagc
     2101 aaaccgagat cgccgccaag atgaaggaga tcctggagca catggtcaag tccgaaggct
     2161 tccaggaagc ggtcaacctg ctctacgaaa tccagaaggc ccagaccgac gtccacgagc
     2221 agaccaacaa agagcgccag gagcggatc
//
LOCUS       DASUGB010000298         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1978876, whole genome shotgun sequence.
ACCESSION   DASUGB010000298 DASUGB010000000
VERSION     DASUGB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978876"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="VFV87_05700"
     CDS             <1..127
                     /locus_tag="VFV87_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX5103282.1"
                     /translation="VSWLHNFRRLRIRWERRDDIHEAFMTLAEGLICFSIICGLC"
     gene            complement(133..492)
                     /locus_tag="VFV87_05705"
     CDS             complement(133..492)
                     /locus_tag="VFV87_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5103283.1"
                     /translation="MKAKREFRANPKSPAERAREKSLRERLQREKPSLDELTRSGECD
                     PHSVMSMGMYFDVQRALQALKRDRQRRGLTIGDVAERSGLDRAVVSRLENGKQDNPTV
                     ATLMRYAEAIGRRMLWT"
     gene            complement(636..779)
                     /locus_tag="VFV87_05710"
     CDS             complement(636..779)
                     /locus_tag="VFV87_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103284.1"
                     /translation="MDVTIIWDLEDDPDGNVQHLREHDVSIDEAEEVLLDRQSSRAAS
                     RSS"
     gene            complement(971..1417)
                     /locus_tag="VFV87_05715"
     CDS             complement(971..1417)
                     /locus_tag="VFV87_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5103285.1"
                     /translation="MTRTMTPWTTRLPRLFEFEDFPRWMTEAFGPETGVFGRDGKFLP
                     EVNVTETDKTIDVAVDLPGMKPEDVKVELHEGNLWISGEKKEEKEEKGKTFHRMERRS
                     GAFRRILPLPVEVDPGKVDARFHDGVLTISLPKSEKVAPQKIEVKG"
     gene            complement(1929..2369)
                     /locus_tag="VFV87_05720"
     CDS             complement(1929..2369)
                     /locus_tag="VFV87_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5103286.1"
                     /translation="MIRACDLFALTVALALAISAAASAQDGARASISGLATGRQPRLG
                     IYGKFDDGFGLAVDRVRWGTPAARLGLEAGDVIRAINGHWLRNERDYWRQLRDSKGEV
                     RLVVVDVRTGRLISRNVFIGPRYADFSSSPAAYGGDPNIGRFEK"
     gene            2483..>3901
                     /locus_tag="VFV87_05725"
     CDS             2483..>3901
                     /locus_tag="VFV87_05725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX5103287.1"
                     /translation="MDQLRTILRKVWGFDEFRPLQAEAMQAVLAGRDSVVVLPTGGGK
                     SLCFQAPALAMEGLAVVVSPLISLMKDQVDALADAGVPAACVNSSVSIDEKRRVTAEI
                     KAGRLKLLYLAPETLMTERMLQFLRGTPVSFFAIDEAHCISDWGHDFRPEYRMLKALK
                     ETFPQVGVHAYTATATERVRHDIARELLLQQPEILVGSFDRPNLVYRVARRADMWEQV
                     QEVIARHADEAGIIYCLRRKDVDSLAASLAEAGHSALPYHAGLEDATRKKNQDEFAND
                     RAKIIVATVAFGMGIDKSDVRYVIHAGAPKSLEHYQQESGRAGRDGLEAECVLLHNGA
                     DFLAWKRLQASLPLAANRIALEVLKGIEDFCTSVTCRHRSIVEYFGQHHSDENCGACD
                     VCLGEIAVQDGALVIAQKILSSVLRQGQNFGGEYTAQVLTGSREQRIVENGHDQLSTW
                     GLLTEHGKRNVRDWIEQLAGQGF"
BASE COUNT          701 a         1304 c         1178 g          718 t
ORIGIN      
        1 ggtgtcttgg ctgcataatt tccgtcgcct gcggatacga tgggaacgcc gtgacgacat
       61 tcacgaggcc ttcatgaccc tcgccgaggg cctcatctgc ttcagcatca tctgcgggtt
      121 gtgttaaacc ctctaagtcc acaacattcg cctcccgatc gcctccgcat agcgcatcag
      181 tgtggcgacc gtcggattgt cttgtttgcc gttttccaac cggctgacga cggcccggtc
      241 cagccccgac cgttcggcca cgtcgccgat cgtcaatccg cgccgctgcc ggtcgcgctt
      301 gagagcctgc aacgcccgct ggacgtcaaa gtacattccc atcgacatga ccgaatgcgg
      361 gtcacactcg ccgctgcgag tcagttcgtc cagcgagggc ttctcgcgtt gcaatctttc
      421 gcggagcgac ttctcgcggg cccgctccgc gggagacttc ggattggctc ggaattcgcg
      481 tttggctttc atcagcttct ccgtttcttc cgtggagcag gaggcggcgc gtcgtaagca
      541 gtcaccggcc gcaacgtcag aggatcgtca tccaccagtt cgtacacgac cgccaaatag
      601 cggccagtcg aagtccaccc gaaaacggtc ggcaatcagg acgagcggct tgccgcgcgg
      661 ctgctctggc ggtcgagcag cacttcttcg gcctcatcga tcgaaacgtc gtgttcccgc
      721 agatgctgga cgttcccatc gggatcgtct tccaggtccc agatgatcgt tacgtccatg
      781 cgggaacgct cgcgctactg gcatagaaac cctagcccga catgacttat aagtcaactc
      841 caacagccag cgaagaggca accgcagtgc gccgcggcct gtcgggggag gatcagcgca
      901 aaaaaccggc ccccggccac atagccgggg gccttcgcgc tgccgctgcc atcgagccat
      961 caaagaccga tcagcctttg acctcgatct tctgcggcgc gactttctcg ctcttgggca
     1021 gcgagatcgt cagcacgccg tcgtggaacc gggcatcgac cttgcccgga tcgacttcca
     1081 ccggcagcgg caggattcgc cggaacgcgc cgctacggcg ctccatccgg tgaaatgtct
     1141 tgcctttctc ctccttctct tccttctttt cgccgctgat ccagaggttc ccctcgtgca
     1201 gctcgacttt cacatcctcc ggtttcatgc cgggcagatc gaccgcgacg tcaatcgtct
     1261 tgtcggtctc agtcacattc acctcgggca ggaacttgcc gtcccgcccg aaaacgcccg
     1321 tctccggccc gaaggcttcc gtcatccacc tggggaagtc ctcgaactca aacaaccgcg
     1381 gaagacgggt ggtccagggt gtcattgtgc gtgtcatgac tggtcctccc tcttcttgat
     1441 ctcgtcacgc acagcgtggc gaacttgtaa ttaccagccg cgccagccgt ctacgtcgag
     1501 aaaccggcgc ggccacatcg aaatgtactg tttctcgtcc ctctcctttt tctacgtctt
     1561 cggattccgg atcgttcgtc agaaagcggg ccgatgccgc gtcccaaggc tcgcgcttcc
     1621 ccggtgccgc ggcatcgggc ccgaagtgcg aaatccgttc gcccaaccgc ctcgtttggg
     1681 gcgacttgga caagcgattc cacccaagca ataggcgttg cgaatcgagt gccgaagtgg
     1741 attctggcca cgcgcgaatc ggcgttatct ggccggggcg gcctaagtcg ttgctaagtc
     1801 gcctcttaga gagagatgtc tgctaacgga ttcaggcggc gtgccgcccg gaaaggaatg
     1861 caaagcaggg ttagtgtctc aggcgcgggc gcgcggtggg cgcgctctcg cgcaccacga
     1921 gcggcctctc atttttcaaa tctgccgatg ttcggatcgc cgccgtaagc cgcggggctg
     1981 ctgctgaagt cggcatagcg cggaccgata aagacgtttc gggagatcaa gcgaccggtc
     2041 cgcacatcga ccaccaccag ccgcacctct cccttcgaat cgcgaagctg ccgccaataa
     2101 tcgcgctcgt tccgcagcca gtggccgtta atcgcccgga ttacgtcccc cgcttccaga
     2161 ccgagccgcg ccgccggcgt tccccaccgc acgcgatcga ccgccaggcc aaagccgtcg
     2221 tcgaacttgc cataaatccc cagccgcggc tgccggccgg tggcaaggcc cgaaatggaa
     2281 gcccgagcgc cgtcctgggc gctggcagcc gcggagatgg ccagggccag agcgacggtc
     2341 aatgcgaaca agtcgcaggc gcgaatcatc acctgtattc tcaccttcct agcgaccagc
     2401 aggcaaccac cggctccgca agcgacatcc accggcgcgt gctacaatcc gcggcagccg
     2461 ctcagctctt cagggcccgt ccatggacca gcttcgcacg atcctgcgca aggtctgggg
     2521 cttcgatgaa tttcgcccgc tgcaagccga agcgatgcag gcggtcctcg ccggccgcga
     2581 ctcggtcgtc gtcctgccca caggcggagg caaatcgctc tgcttccagg cccccgcgct
     2641 ggcgatggaa gggctggcgg tcgtcgtctc gccgctgatt tcgctgatga aggaccaggt
     2701 cgatgccctg gccgacgccg gcgtgcccgc cgcctgcgtc aacagctcgg tttcgatcga
     2761 cgaaaagcgc cgcgtcaccg ctgaaatcaa agcgggccgg ctgaagctgc tgtatctcgc
     2821 gcccgagacg ctgatgaccg agcggatgct gcagttcctc cgcggcacgc ccgtcagctt
     2881 cttcgcgatc gacgaggccc attgcatcag cgattggggg cacgattttc ggcccgaata
     2941 ccggatgctg aaggcgctga aggagacgtt tccgcaggtc ggcgtccatg cctatacggc
     3001 gaccgcgacc gagcgcgtgc ggcacgacat cgcccgcgag ctgctcttgc agcagcccga
     3061 aatcctcgtc ggctcgttcg accggccgaa cctcgtctat cgcgtggccc gccgcgcaga
     3121 catgtgggag caggtccagg aagtaatcgc ccgccatgcg gacgaagcgg gcatcatcta
     3181 ctgcctccgc cgcaaggatg tcgatagcct ggccgccagc ctggccgagg ccgggcattc
     3241 cgcgctgccg tatcacgcgg gcctggagga cgcgacgcgg aagaagaacc aggacgagtt
     3301 tgccaacgac cgggcgaaga tcatcgtcgc caccgtcgcg tttggcatgg ggattgacaa
     3361 gtccgacgtg cgatacgtga tccacgccgg cgcgccgaaa tcgctggagc attaccagca
     3421 ggagagcggc cgcgcgggcc gcgacggctt ggaagccgag tgcgtattgc tgcacaacgg
     3481 ggccgatttt ctcgcctgga agcgcctgca ggcttcgctg ccgctggcgg ccaaccggat
     3541 cgccctggaa gtgctcaagg gaatcgagga tttctgcacg agcgtcacct gccggcaccg
     3601 gtcgatcgtc gaatacttcg gccagcacca ttccgacgag aactgcgggg cctgcgacgt
     3661 gtgcctgggc gagatcgccg tgcaggacgg agccctcgtg atcgcacaga agattctgtc
     3721 gagcgtgctg cggcaggggc agaactttgg cggcgaatat acggcccagg tgctcaccgg
     3781 ctcgcgcgag cagcggatcg tcgaaaatgg ccacgaccag ctcagcacgt ggggcctctt
     3841 gaccgagcat ggcaagcgga acgtccgcga ctggatcgag cagcttgccg gtcagggctt
     3901 c
//
LOCUS       DASUGB010000299         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1145035, whole genome shotgun sequence.
ACCESSION   DASUGB010000299 DASUGB010000000
VERSION     DASUGB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145035"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFV87_05730"
     CDS             complement(<1..697)
                     /locus_tag="VFV87_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5103288.1"
                     /translation="MANAELISANPVACDCCQARGPEYACVVDTLDTSAVRFKGWIDR
                     LIAALMLIPGLPMIGILVAAVRLTSKGPGIYRQVRVGKGGRIFTMYKLRSMRVDAEAG
                     SGPVWASLTGDSRVTPLGFWLRRLHLDELPQLFNVLKGEMSLVGPRPERPEFVKVLAE
                     KIDGYLDRLQVAPGITGLAQINLPPDTDLDSVRRKLHLDCEYIRDAGLLFDLRIIACT
                     LLRVVGLRGGRAVA"
     gene            complement(720..1394)
                     /locus_tag="VFV87_05735"
     CDS             complement(720..1394)
                     /locus_tag="VFV87_05735"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HEX5103289.1"
                     /translation="MKYPVIAVYVLLCAGTLAGGLVQRRSSFGITPQQLLDQAAHQLA
                     RPLPERFGEWRLLQETELEERVADMLRCPAYVNRVYVHDRTGDRVSVIVLVGPSGPIA
                     VHTPEICYSSRDFKIISERVPVSLDANQQPDQTFWEVGFESNDLSAAKMRVLYAWSDG
                     SRWNASQQPRWEYGSLPYLYKIQIAGPESEPGSEFDPCHDFLAQFINQLDQHLVVAPA
                     AKPWPL"
     gene            complement(1402..>2506)
                     /locus_tag="VFV87_05740"
     CDS             complement(1402..>2506)
                     /locus_tag="VFV87_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103290.1"
                     /translation="AMVEFLARNGPRSETELWIEKLESLAPAPPKNDGDLMARIVELR
                     IKLNTLDQCTNWLDRLEAAEPGRIRPFILRAKLLVAQGQPQEAIANLDAKADELLLAA
                     KEQKEQVEIVKAVGDFYFSSDLATSAETWYGRLAKLDPEQAPLAILALAREQRMTEAI
                     RLCIARSQNDETARYAIVAAAVLAESPAESDASALADPLFASALKRFPGNINLLYSLG
                     TLRVAQGRYGEAVNLFRETITKSPRHVPALNNLALILSESPATRAEALQLIDRAIAVV
                     GENPSLLDTKGAILIYGGQASAALPLLQTAARGSAVDPRHQLHLAAAYQGTAQSQLAR
                     DMLKLALDQKIEKLILTQTDKNLLADLRGRLGL"
BASE COUNT          477 a          722 c          833 g          474 t
ORIGIN      
        1 gcgcgacggc gcggccgccg cggaggccga cgactcgcaa gagcgtacag gcgataatcc
       61 gcagatcgaa cagcaaaccg gcgtcgcgga tgtactcgca atcgaggtgg agcttgcggc
      121 ggacgctgtc gaggtcggta tcggggggca aattgatctg ggcgagaccc gtgatgcccg
      181 gcgcgacctg caggcggtct aagtagccgt cgattttttc agcgaggact ttgacgaact
      241 cgggccgctc ggggcgcgga ccgacgagcg acatttcgcc cttcagcacg ttaaaaagct
      301 gcggcagctc gtccaggtgc aggcggcgga gccaaaagcc gagcggcgtg acgcgcgaat
      361 cgcccgtcag gctggcccag acggggcccg agccggcttc ggcatcgacc cgcatgctgc
      421 ggagcttgta catcgtgaag atccgcccgc ctttgccgac acggacctgg cgatagatgc
      481 cggggccttt cgacgtcagc cgaacggcgg ccacgaggat gccgatcatc ggcagccccg
      541 gaatgagcat cagggcggcg atcagtcgat cgatccagcc tttgaagcga acggcggaag
      601 tgtcgagggt atcgacgacg caggcatatt ccggtccgcg agcctggcag cagtcgcagg
      661 cgaccgggtt ggcggaaatg agttctgcat tggccatgga ttctttcctt cgcaaggtac
      721 tacaggggcc aaggtttggc agcgggggca acaacaagat gttgatcgag ctggttgata
      781 aactgggcta aaaagtcgtg gcagggatcg aattcgctgc ccggctccga ttcgggaccg
      841 gcgatctgaa tcttgtagag gtagggaagc gagccatatt cccagcgcgg ctgctggctg
      901 gcgttccagc ggctgccgtc gctccaggcg tagagcacac gcatcttggc cgcactcaag
      961 tcgttggact caaagccgac ttcccagaat gtctggtcag gctgctggtt ggcgtcaagc
     1021 gagacgggca ctcgctcact gatgattttg aagtcgcggc tggagtagca gatttcagga
     1081 gtgtgcaccg cgatgggtcc cgacggcccg acgagcacga tgacggacac gcggtcgccc
     1141 gtgcgatcgt ggacatagac gcggttgacg taagcggggc agcgcagcat gtcggccacg
     1201 cgctcctcga gttcggtctc ctgcagcagg cgccactcgc cgaatcgttc cggtagcggg
     1261 cgcgcgagct gatgcgcggc ctggtcgagg agttgctgtg gggtaatgcc gaaactcgaa
     1321 cggcgctgca ccaatccccc ggccaaagtg ccggcgcaca ggaggacata cacagctatg
     1381 acggggtact tcatgctctg atcaaagccc aagccgaccg cggaggtcag caagcaggtt
     1441 cttgtctgtt tgggtgagaa tcagtttctc gatcttctga tccaatgcca gcttgagcat
     1501 gtcgcgggcc agttggctct gtgctgttcc ttggtaggcc gccgcgaggt gaagctggtg
     1561 gcggggatcg accgcagacc cgcgggcagc ggtttgcagg agcggaagcg ccgccgatgc
     1621 ctggccgccg tagatgagaa tcgcgccctt ggtatcgagc aggctgggat tctcgccaac
     1681 gacggcgatc gcccgatcga tcaattgcag cgcctccgcg cgggtcgcag gcgattcgga
     1741 aagaatcagc gccagattgt tgagcgccgg cacatggcgc ggactcttgg tgatggtctc
     1801 gcggaacaag ttgacggcct ctccatatct gccctgggcg acgcgcagcg tgccgaggct
     1861 atacagcaga ttgatgttgc cgggaaatcg cttgagagcc gacgcaaaca atggatcggc
     1921 cagtgcgctg gcatcggatt cggctggcga ttccgccagc acggctgccg cgacgattgc
     1981 atagcgagcc gtttcgtcgt tctgactgcg agcgatgcac aagcgaatgg cttcggtcat
     2041 acgttgctct cgggcgagcg ctaaaatcgc gagtggcgct tgctcgggat cgagtttcgc
     2101 cagcctcccg taccatgtct cggcgctggt agccaaatcg ctgctgaagt aaaaatcgcc
     2161 cacagccttg acgatctcca cctgctcttt ctgttccttc gcggcgagaa gcaattcgtc
     2221 agccttggcg tccaaattgg cgatcgcctc ctgaggctgg ccttgcgcca cgaggagttt
     2281 ggcgcgaaga atgaatggcc gtatgcggcc tggctcagcc gcttcgagcc ggtcgagcca
     2341 atttgtgcac tggtctagcg tgttgagctt gatccgcaac tcaacgatcc gagccatcag
     2401 gtcaccgtcg ttcttaggtg gcgcgggggc aagagattcg agtttctcaa tccacaattc
     2461 tgtttcggac cgcgggccgt tacgggccag gaattcgacc atcgcg
//
LOCUS       DASUGB010000300         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1145042, whole genome shotgun sequence.
ACCESSION   DASUGB010000300 DASUGB010000000
VERSION     DASUGB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145042"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFV87_05745"
     CDS             complement(<1..974)
                     /locus_tag="VFV87_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5103291.1"
                     /translation="MFDLLLRGGTVIDGSGRPRVSGDVGIREGRIAAIGRLADAAATN
                     VFDCRGKIVAPGLIDVHNHSDGWMLKFPHQTWKTSQGFTTEVLAADGIGYAPVSEANW
                     RQWLYYLRSLNGLSLEDYRGWHSLDDYMRLLDGKNVQNAATHVPYANVRTLVCGFGRA
                     APDDFQMRIIRREIARGMDEGAVGLSTGLDYIAQHYASTDEIANACTAVVPYGGLYVT
                     HMRYKAGLVPALREAVEIGRRTGVKVHISHLKGGSEQEVEEVLSYIDREARQQVDFSF
                     DVYPYQRGSTMLNFLLPYDVWEDGPLGVLARLDRPETRAAFAAGLESHA"
     gene            1162..1821
                     /locus_tag="VFV87_05750"
     CDS             1162..1821
                     /locus_tag="VFV87_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103292.1"
                     /translation="MRHIPAWLWWLGAAGGFGMLAGCALLYGNEWWLSGIVTVSIFAW
                     LAGVLAAIYLAARRRAVILGAVVGSFLYLLLAVGPWFSSSVGPWLLTTRALTTIETQW
                     LHREPPQQQVVYQAVPTPYNTGWQSYPGGWSGGSPYINSGSTAILTGYVASGSVAVPS
                     GPSLFVTTGHWLCGWLAAVAGALAAAWISRRAGRQQKEAAPGSTADQREGENPFAESP
                     Q"
     gene            1818..2399
                     /locus_tag="VFV87_05755"
     CDS             1818..2399
                     /locus_tag="VFV87_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103293.1"
                     /translation="MTLRFSLLSLIGLTTLAGLASAALVQPGVGWTSVVVSLTVAMFA
                     WQVLRAILTNDDSRAAAIGWIFFAAAYLAIVLGPWLSSRLGPQLLSSRALVHAQVNWR
                     KESINLPLDEYQRRVGLDLWGLPVEGRAPSLIFDTSGSALWTTQVISAGPSPTDSANH
                     FQLSGHWLCAWLAGWIGAVLAAQFWRNGRSRRR"
     gene            2501..3799
                     /locus_tag="VFV87_05760"
     CDS             2501..3799
                     /locus_tag="VFV87_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5103294.1"
                     /translation="MSDFSRRSFLESSAAAGAAWAAGSYFVSEAQAQEPKSANERIAV
                     AIMGVNGRGGGLAKGYMAQTNCEIAYICDVDENAMARTAKTVGDGQERKPQAVKDFRQ
                     ALDDKSLDVLVCAAPNHWHAPATILGCSAGKHVYVEKPCSHNPREGEMAVEAARKYKR
                     VVQMGTQRRTWPGVVEGIKKVHDGAIGPVHYSRTWYTNRRPSIGFGKPATIPTWLDWE
                     LWQGPAPEKPFVDNVVHYNWHWKWHWGNGELGNNGIHALDVARWGMQVDYPVRVTSGG
                     GKYRHEDDQQTPDTHLVTYDFPGKKTITWEGLSWSPYGPGGSSFGISFHGEEGTLVIQ
                     DSGYTIYDMKNKESDSGKGNAGDTEHIANFLACVKSGARPNADIEHGHKSTLLCHLGN
                     IAHRVNRVLTTDAKNGHIQGDDEAMALWQREYRKGWEPVV"
BASE COUNT          676 a         1246 c         1375 g          681 t
ORIGIN      
        1 gcgtgcgatt cgaggccggc ggcgaacgcg gcacgggttt ccggccggtc aagccgagcg
       61 agcacgccga gcggcccgtc ctcccagacg tcgtacggca ggaggaaatt gagcatcgtc
      121 gagccgcgct ggtagggata aacgtcgaac gagaaatcga cctgctggcg ggcctcgcga
      181 tcgatgtagc tcagcacctc ctcgacttcc tgctcgctgc cccctttgag gtgcgaaatg
      241 tgtaccttca cgcccgtgcg gcggccgatt tcgaccgctt cgcgcagggc gggcacgagg
      301 cccgccttgt aacgcatgtg cgtgacgtag agtccgccgt agggcacaac cgccgtgcag
      361 gcgttggcga tttcgtcggt gctcgcgtaa tgctgcgcga tgtagtcgag acccgtcgag
      421 aggccgacgg cgccttcgtc catgccgcgg gcgatttcgc ggcggatgat ccgcatctga
      481 aaatcgtccg gcgccgcgcg gccgaacccg cagacgagcg tgcggacgtt ggcgtacggc
      541 acgtgcgtgg cggcgttctg cacgtttttg ccgtccagca gccgcatgta atcgtccagg
      601 gagtgccagc cgcgataatc ctccagcgac aggccgttga gcgaccgcag gtaatagagc
      661 cactgccgcc aattggcctc gctcaccggg gcatagccga tgccgtcggc cgcgagcacc
      721 tcggtggtga agccctggct ggttttccac gtctggtgcg ggaatttcag catccagccg
      781 tcgctgtggt tgtggacgtc gatcagtccg ggagcgacga tcttgccgcg gcagtcgaag
      841 acgttcgtgg cggcggcatc ggcgagccgg ccgatggcgg caatgcggcc ctcgcgaatt
      901 cccacgtcgc cagaaacgcg cgggcggccc gagccgtcga tgacagtgcc gccgcggagg
      961 aggaggtcga acaaggaagg tcctttcgtg gggcgggctt ccagcctgcc aaattggtcg
     1021 atcgcggcag gcaagatgcc tgccccactc cggcaggcaa gatgcccgcg ccacaggcgc
     1081 ttgcctaagt agccgccgcg ggggaaattg taatcggacc agcggcacga gcgggaggca
     1141 gggccccacg gagcacgagt gatgaggcac attccggcgt ggttgtggtg gctgggcgcg
     1201 gcgggcggct ttgggatgct ggccgggtgc gccctgctgt atggcaacga gtggtggctg
     1261 agcgggatcg tgacggtgtc gatcttcgcc tggctggcgg gcgtgctggc ggccatctat
     1321 ctggctgcga ggcggcgggc ggtgattttg ggtgcggtcg tcggcagctt tttgtatctg
     1381 ctgctggccg tcggtccgtg gttcagctcg agcgtcgggc cgtggctgtt gacgacgcgg
     1441 gcgttgacga ccatcgaaac ccagtggctg caccgcgagc cgccgcagca acaggtggtt
     1501 tatcaggcgg tgcccacgcc gtacaacacc ggctggcaga gctatcccgg cggctggagt
     1561 ggcggcagcc cctatatcaa cagtggctcg accgccatcc tgaccggcta tgtggcgtct
     1621 ggctccgtgg ccgtgcccag cgggccgagc ctgttcgtga caaccggcca ctggctgtgc
     1681 ggctggctgg cagcggtggc gggggccctg gcggcggctt ggatttcgcg gcgggcaggg
     1741 cggcagcaga aggaagcggc tcctggcagc acggctgacc agcgcgaggg cgaaaacccc
     1801 tttgcggagt ctcctcaatg accctccgct tctctctctt gagcctgatt ggcctgacga
     1861 cgctcgccgg gttggcttct gcggctctcg tgcagccggg cgtcggctgg acgagcgtcg
     1921 tcgtttcgct gaccgtggcg atgtttgcct ggcaggtgct gcgggcaatt ctcacgaacg
     1981 acgattcgcg agccgcggcg attggctgga tcttcttcgc ggcggcgtat ctggccatcg
     2041 tcctgggccc gtggctcagc agccggcttg gcccgcagct tctcagcagc cgggccctcg
     2101 tccacgccca ggtgaactgg cgcaaggaaa gcatcaacct gccgctcgac gagtatcagc
     2161 gacgggtggg gctcgacctg tggggcctgc ccgtggaggg cagagcgccc tcgctaatct
     2221 ttgacacgag cggcagcgct ctttggacca cgcaggtgat ttctgcaggg ccctcaccca
     2281 ccgactctgc caatcacttt cagctctccg gccactggct ctgcgcctgg ctcgccggct
     2341 ggatcggcgc ggtgctggcg gcgcagtttt ggcggaatgg tcgctcccgc cgccgttgag
     2401 tttgctcggt gggagcgatt gtggcgcgtg aatctggctg cggcttgtgc tacgctatcc
     2461 gcagctttcc tctcgatccc accgcttgcg gagccttgcc atgtccgatt tttctcgccg
     2521 ctcgtttctc gaatcgtctg ccgctgccgg ggctgcttgg gcggcaggtt cgtactttgt
     2581 ctccgaggcg caggcgcagg agcccaagag cgccaacgag cggatcgccg tggcgatcat
     2641 gggggtcaac ggccgcgggg gcgggctggc caagggttac atggcccaga cgaactgcga
     2701 aatcgcctac atctgcgacg tcgatgaaaa cgcgatggcc cgcacggcaa aaaccgtcgg
     2761 cgacggccag gagcgcaagc cgcaggcggt gaaggacttt cgccaggccc tggacgacaa
     2821 atcgctcgac gtcctcgttt gtgccgcccc gaatcactgg cacgccccag cgacgatcct
     2881 cggctgctcg gcgggcaaac acgtgtatgt cgagaagccg tgcagccaca acccgcgcga
     2941 aggggaaatg gcggtcgaag cggcccgcaa atacaagcgg gtcgtgcaga tggggacgca
     3001 gcggcgaacc tggccgggcg tcgttgaggg gatcaagaag gtacacgacg gcgcgatcgg
     3061 ccctgttcac tactcccgca cctggtacac caaccgccgg ccgagcatcg gcttcggcaa
     3121 gccggcgacg attccaacct ggctcgactg ggaactctgg caagggccag cgccagagaa
     3181 gccgttcgtc gataacgtcg tccactacaa ctggcactgg aagtggcatt gggggaacgg
     3241 agagctgggc aacaacggca tccacgccct ggacgtggcc cgctggggga tgcaggtcga
     3301 ttacccggtc cgcgtgacca gcggcggcgg caaataccgg cacgaagacg accagcaaac
     3361 gcccgacacg cacctggtca cgtacgactt cccgggcaag aagacgatta cctgggaggg
     3421 gctgtcgtgg tcgccctatg gccccggcgg gtcgagcttc ggcatcagct tccacggcga
     3481 agaggggacg ctcgtcatcc aggacagcgg ctatacgatt tacgacatga agaataagga
     3541 gagcgactcc ggcaagggga acgccggcga tacggagcac atcgccaact tcctggcatg
     3601 cgtcaagagc ggggcgcggc ccaacgccga catcgagcac ggccacaaga gcacgctgct
     3661 gtgccacctg ggcaatatcg cccaccgcgt caatcgcgtg ctgacgaccg acgccaagaa
     3721 cggccacatc cagggcgacg acgaggccat ggccctctgg cagcgcgaat accgcaaggg
     3781 ctgggagccg gtggtgtagc ggggcgctcg cgaattgctc ttagccggaa ccaaacctgg
     3841 ttccggctaa tctgcgagtt gatcttgtcc attacgctgc aatgcgaagg caccatgccg
     3901 atttccattc attgcccggc gtgtaaggcc aaactgaacg ccccagaaag cgcggtgggc
     3961 aagcgcctga agtgcccg
//
LOCUS       DASUGB010000301         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_728765, whole genome shotgun sequence.
ACCESSION   DASUGB010000301 DASUGB010000000
VERSION     DASUGB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728765"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="coxB"
                     /locus_tag="VFV87_05765"
     CDS             complement(<1..977)
                     /gene="coxB"
                     /locus_tag="VFV87_05765"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5103295.1"
                     /translation="MLLLPSVVAAADDVYSTISPESQDGRDIQFLYKLIFWLALIVFI
                     GVQLAIVYSVMRYRRRSESEPRPEQIHGNQKLEVIWTIIPALVLVAIFIPTVRTMYRI
                     DDRANDGAYTIEVYGKQWWWEVHYTQPDTVAGVVTANEFYIPVNTRVRIELMSSNVIH
                     SFYVPQLAGKMDVMPGHKNVMSVEADKPGMYYGECAEYCGESHAYMRFKIMAVPQDQF
                     DLFISGWKQGASSQAASMAPNGDVDSVPQALGLCLGCHRIGGVPNNPSGQPLPDPGSG
                     LAGGTQPNQILGPNLSMLACRTTLAAGIVPNDDQHLREWLHNPGGVKQG"
     gene            1523..1735
                     /locus_tag="VFV87_05770"
     CDS             1523..1735
                     /locus_tag="VFV87_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103296.1"
                     /translation="MPITLIVIGALLAVGSIFADSLGFTWGGEGYGWKQMLATITGLV
                     IALAGVGLLTRPQPKSRKRRSPAQEA"
     gene            complement(2045..2443)
                     /locus_tag="VFV87_05775"
     CDS             complement(2045..2443)
                     /locus_tag="VFV87_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103297.1"
                     /translation="MSKAKWWAIGAGVAFVVLTAVAVWALYQAGGEDQSALERLRDIS
                     IIFIVALSLVTVLLLAAITAALGYLTFQVKDRMIPLLEELTGTARRIRGTTEFMSEEA
                     VKPFLAVAGTYAKMRGTMKSFVSRGNGSKS"
     gene            complement(2440..>2656)
                     /locus_tag="VFV87_05780"
     CDS             complement(2440..>2656)
                     /locus_tag="VFV87_05780"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX5103298.1"
                     /translation="YRGMPVPCTVCPGATHGEVEVELRGTAAELPIASPGQAIVFYRG
                     DEVLGGGTIRHVEREAELSGRTSEVAE"
BASE COUNT          501 a          844 c          830 g          481 t
ORIGIN      
        1 tgaccctgct tgactccgcc cgggttgtgg agccactcgc gcaggtgctg atcgtcgttc
       61 gggacgatgc cggccgccaa cgtggtgcga caggcgagca tgctcaggtt cggaccgagg
      121 atctggttcg gctgggtacc gccggcgagg cccgatcccg gatccggcag gggctggcca
      181 ctgggattgt tgggcacgcc accgatccgg tggcagccaa ggcagagacc gagcgcctgc
      241 gggacgctat cgacgtcccc attcggcgcc atcgatgctg cctgcgagct ggcgccctgt
      301 ttccagccgc tgatgaagag atcgaactgg tcctgcggaa ccgccatgat cttgaaacgc
      361 atataggcgt gcgattcgcc gcagtattcg gcgcattcgc cgtagtacat gcccggtttg
      421 tccgcctcga cggacatcac gttcttgtgg ccgggcatca cgtccatctt gccggcgagt
      481 tgcggcacat aaaaggagtg gatcacgttc gaagacatga gctcgatgcg aacgcgggta
      541 ttgaccggga tgtagaactc gttcgcagtg accaccccgg ctacggtgtc gggctgcgtg
      601 tagtggactt cccaccacca ctgcttgccg tagacctcga tggtgtaggc gccgtcattc
      661 gcgcggtcgt cgatgcggta catcgtgcga accgtcggga tgaagatggc gaccagcacg
      721 agggccggga tgatcgtcca aatcacctcc agcttctggt tgccatggat ctgctccggc
      781 cggggctcgc tctcggagcg gcgccggtag cgcatgaccg aatagacaat tgcgagctgg
      841 acgccgatga atacgatcag tgccagccag aagatcagct tgtaaaggaa ctggatatcg
      901 cggccgtcct gggactccgg cgagattgtc gagtagacgt cgtcggcagc ggcgacgacg
      961 cttggcagga gcagcatggc tcccataagc accatcgaca acaacacgac aagcctgctc
     1021 cgggtcgact ttcgaatggg tgctctgtgc cgctcccgac ccctcatgct gcctcctgct
     1081 cgcaccgaat ccctcgtacc tcgctcagac gacaaaaaac gcgcactctt cgtcgccgaa
     1141 ccgctgcggg aacggcgcaa gagtcacgcg aattcgtgtc atgccgaaca tatctgtttc
     1201 caatcgctat cgagatcagg cagatccgtg aactccgcga ctggccccgc tccccaacga
     1261 gtgccaaagg cgcgtcaggg cgcggtgaac cgcgctcggc gcttgcctca tggcgcgcat
     1321 cgtacacgat tgcgatcgct cctggtcaag cattcctacg caggcgtcgg gcatgagtgc
     1381 gaaagtccgc aaactggact accatcgacg ggaagggatg cgtgcgcagg cacagggcgg
     1441 acaagcggca ggcgggccaa cgagtcaatc gttcaggacg tgaatggaat ctcagccaac
     1501 ccggcaaact ggatatcggt ggttgccgat caccctgatc gtgatcggcg cgctcctggc
     1561 ggtgggctcc atcttcgcgg actcgctcgg gttcacctgg ggcggagagg gctacggctg
     1621 gaagcagatg ctggcgacca taacgggact ggtaatcgcg ctggccggtg tcggtttgct
     1681 gacccgccca cagccaaagt cccgcaagcg gcgtagcccc gcccaggaag cctaagcagg
     1741 tttctgatga tgtgcctttc gtctgacgta gggcggcgtg caaggagatt ggccgccttg
     1801 gcaatgtagc gcggggttac cacgccccgc ccggcccaga cggcgatcca tttggcgcgg
     1861 aaaccacccc gatcagccgc attccaccgg caaccgtgcg gggaaggaga ccagcggggc
     1921 gtgttcctct ctccgactct ctcctcaagg aaagagggca tccccgcgct ccgtctccac
     1981 gttggccaac gttgacggaa tacagcactc gaccaggtcg cattccgtgg ctgcgaattc
     2041 cgcgttagct cttgctgccg ttgccgcgcg agacgaagga cttcatcgtg cctcgcatct
     2101 tggcataggt gccggcgacg gcaaggaagg gcttgacggc ctcttcgctc atgaattcgg
     2161 tcgtgccccg gattcggcgg gccgttccgg tcaactcctc gagcagcgga atcatccggt
     2221 ccttgacctg gaaggtcagg tagcccagcg cggcggtaat ggccgccagg agcaggaccg
     2281 tgaccaggga tagcgcaaca atgaagatga tggagatgtc gcggagtcgc tcgagcgcgg
     2341 actggtcctc accgccggcc tggtacagcg cccagaccgc cacggcggta agcacgacga
     2401 aggccacacc cgcaccgatc gcccaccatt tcgccttgct cattcagcta cctcgcttgt
     2461 gcggccgctg agttcggcct ctcgctcgac gtgacgaatc gtaccgcctc cgagaacttc
     2521 gtcgccgcga tagaacacaa tcgcctgtcc cggtgacgca atcggcaact cggctgccgt
     2581 tccccgcaat tcaacttcga cctcaccgtg ggtcgcgccc gggcaaaccg tgcatggcac
     2641 cggcataccc cgatag
//
LOCUS       DASUGB010000302         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_451270, whole genome shotgun sequence.
ACCESSION   DASUGB010000302 DASUGB010000000
VERSION     DASUGB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451270"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFV87_05785"
     CDS             <1..774
                     /locus_tag="VFV87_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5103299.1"
                     /translation="YDNVHVTVGDGYQGWPQNAPFDKIIVTCSPEKVPQPLIEQLADG
                     GRMVVPVGERYSQTLYLFRKKEGKLESEALIPTLFVPMTGRAEAARAVKPDPANPKLV
                     NGSFEEEAFKGGGQPGWYYERLVTWEADEKSPDGSHHLRFENSEPGLDAHLLQGLAID
                     GRKVNELEISGWVKLENVVAGTGREQSPAIGLTLYDEQRRDLGTLVLGPFRGTADWHE
                     ETKTFRIPPAAREGIFRIGLFGATGQAAFDKLQIKKVDR"
     gene            complement(854..1786)
                     /locus_tag="VFV87_05790"
     CDS             complement(854..1786)
                     /locus_tag="VFV87_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5103300.1"
                     /translation="MRIVSLISSGTEILFALGLGGQVVAASHECDFPSAAAMLPRATR
                     SWIDAHQASGQIDAQVHDRLAAGKSLYEIDAELICRLAPNLIVTQAQCDVCAVRYADV
                     ENLVQSRSELRGAQIVALQPRSLDDMLEDVQRIGRATRAEVAAGRLVTQLRQRIERVA
                     SCAARAAPDQRPRVVCIEWTNPLMAAGNWTPALIELAGGRPGLATAGQHSGYVSWDDV
                     REFDPQVLLVAPCGFDLPRSRIEAEALRCLPGFIELSAVRSQRAFVLDGNAYLNRSGP
                     RLVDSLEILAHLIQPERFPPPPLADAWEPLASGP"
     gene            complement(1827..>2946)
                     /locus_tag="VFV87_05795"
     CDS             complement(1827..>2946)
                     /locus_tag="VFV87_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103301.1"
                     /translation="NGIEVDQDRALGPDSKPDNWLVARLWFDKATNAVTFSGKPIRGK
                     SPLLFYSGGPMSQINGAAAMQKDGYFFQRAQIHWQMAADEWFDYGNRELPATAGYNIR
                     LNDMEAVQARVEELRGEVDKLAPGVRDQLRKDKIAKLTPELRAAYEKPEKERSPEEVG
                     RFYQAQFQVIVADREVLDAAPREAKPQVRGLIDQIEADTGLAESISRNRNIVNFEYWR
                     TRCQAERTDLAQGARKDVYDADKMFASGEKFDAARQTYERSWAAWDKVFAEHPVLMDN
                     AEAQDLIESIEHYRDLLGQLDEKFPVDFPLNRLLERHQEGKQILEQAKLLSDGAGSKT
                     PSDQKPASEKPASEKPADQKPADQKPADVQPTDPKSSA"
BASE COUNT          504 a          952 c          940 g          550 t
ORIGIN      
        1 tacgacaacg tccacgtcac ggtaggcgac ggctatcaag gctggccaca gaatgcccca
       61 ttcgacaaga ttatcgtcac ctgctcgccc gaaaaagtcc cgcagccgct tatcgagcaa
      121 ctggccgacg gcggacgcat ggtggtgccg gtgggcgagc gatactcgca gacgctgtac
      181 ctctttcgca aaaaggaggg aaagcttgag tcggaggcct tgattcccac gctcttcgtg
      241 ccgatgactg gccgggccga agcggcccgc gcggtgaagc ccgacccggc caaccccaaa
      301 ctcgtcaacg gcagctttga agaggaagcc tttaaagggg ggggccagcc gggctggtat
      361 tatgaacgac tggtcacgtg ggaggcggac gagaaatcgc ccgacggcag ccaccacctg
      421 cgatttgaaa acagcgaacc cggactggac gcacatctgc tgcagggatt ggcgatcgac
      481 gggcgcaagg tgaatgagct ggagatttcg ggctgggtga agctcgagaa tgtcgttgcg
      541 ggtacgggcc gggaacaatc gccagcgatc ggcctgacgc tttacgacga gcagcggcgc
      601 gacctgggaa cgctggtcct gggcccgttt cgcggcacgg ccgactggca cgaggagacg
      661 aagacgtttc gcattccgcc ggccgcccgc gaagggatct ttcgcatcgg cctgttcggc
      721 gccaccggac aggccgcgtt cgacaagctg cagatcaaga aggtggaccg gtagttgttc
      781 gtggttcgtg gttcgtggtc cgtggcctgt tgatgttggt cccgcagtac gctgactgtt
      841 ccctgtccaa gcattaggga cccgacgcga gcggctccca ggcgtcagct agtggcggcg
      901 gcgggaatcg ttccggctgg atcaggtggg ccagaatctc caggctatcg accagccgcg
      961 ggccgctgcg attgagatag gcgttgccat cgagcacgaa ggcccgctgc gaccgcacgg
     1021 ccgagagctc tatgaatccg ggcaggcacc gcagagcctc ggcctcgatt cgcgagcggg
     1081 gcaaatcgaa cccgcacggc gcgaccagca gcacttgcgg gtcgaactcc cgcacgtcgt
     1141 cccaggagac gtagccgctg tgctgtcccg ctgtggccag gcccggtcgt ccgcccgcca
     1201 gctcgattaa ggccggagtc caattcccag ccgccatcag ggggttggtc cattcgatgc
     1261 agaccacgcg cggccgctgg tccggcgcgg cgcgcgcggc gcagcttgcc acccgctcga
     1321 tccgctgccg cagctgggtc accagccgcc cggcagcgac ttccgcgcgg gtggcgcggc
     1381 caatccgctg cacgtcctcc agcatgtcgt ccagcgatcg cggctgaaga gccacgatct
     1441 gtgcgccgcg cagctcggac cgagactgaa ccaggttctc gacatcggcg tagcgcaccg
     1501 cgcagacatc gcattgggcc tgcgtcacga tcaggttggg cgctaaacgg cagatcagct
     1561 ccgcgtcgat ctcatagagc gatttcccag ccgccaggcg gtcgtgaacc tgcgcgtcga
     1621 tttggccgct ggcctggtgg gcgtcaatcc acgaacgggt cgcccgcggc agcatggccg
     1681 ccgctgaggg aaaatcgcac tcgtggctcg ccgcaacgac ctgcccgccc aatcccaagg
     1741 caaacaggat ttccgtgccg ctggaaatga gcgagacgat tcgcacgcgc aggtggcctc
     1801 agcttgagag aaccgggcta cgctgttcac gcactggact tcgggtccgt cggctgcacg
     1861 tctgcgggct tttgatccgc gggcttttga tcagccggct tctcgcttgc cggcttctcg
     1921 cttgccggct tttggtcgga cggggtttta ctgccggcgc cgtcgctgag cagcttggcc
     1981 tgttcgagaa tctgcttccc ttcctggtgt cgctcgagca agcgattgag cgggaaatcc
     2041 accggaaact tttcatcgag ttgccctagc agatcgcggt aatgctcgat cgattcgatc
     2101 aagtcctggg cctcggcgtt gtccatcagc accggatgct cggcgaatac cttgtcccag
     2161 gccgcccagc tgcgctcgta agtctgacga gccgcgtcga atttctcgcc cgaggcgaac
     2221 atcttatccg catcgtacac atccttgcgg gccccctggg cgaggtcggt ccgctccgcc
     2281 tgacagcggg tccgccagta ttcaaaattc acgatattcc ggttgcggct gatgctctcg
     2341 gccaggccgg tgtcggcctc aatctggtcg atcagtccgc ggacctgcgg cttggcctcg
     2401 cggggggccg catccagcac ctcgcggtcg gcgacgatga cctggaattg cgcctgatag
     2461 aagcgcccta cttcctcggg cgaacgctct ttctcgggct tttcataagc ggcccgcagt
     2521 tccggcgtga gcttggcgat cttgtctttc cgcagttgat cgcggacgcc gggcgcgagc
     2581 ttatcgactt cccctcgcag ctcctcgacc cgcgcctgca ccgcctccat gtcgttcagc
     2641 cggatgttgt accccgcggt cgcgggcagt tcgcggttgc catagtcgaa ccactcgtcc
     2701 gccgccattt gccagtggat ttgagcccgc tgaaagaaat agccatcctt ctgcatggcc
     2761 gcggcgccgt tgatctgcga catcggcccg ccgctgtaga acaacagcgg tgacttgccg
     2821 cgaatcggct tgccgctgaa ggtgaccgcg ttggtggcct tgtcgaacca gagacgggcg
     2881 accagccagt tatctggctt ggaatccggt cccagcgccc ggtcctgatc cacttcaatg
     2941 ccgttc
//
LOCUS       DASUGB010000303         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1423078, whole genome shotgun sequence.
ACCESSION   DASUGB010000303 DASUGB010000000
VERSION     DASUGB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423078"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1305)
                     /locus_tag="VFV87_05800"
     CDS             complement(163..1305)
                     /locus_tag="VFV87_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103302.1"
                     /translation="MPLQCPHCANLIQVDSATGGQQLGCPYCQGPFVVPPEDVLMRLL
                     AAAETPPPPPPADELFTLACPACAGPFQVLASMSGQQLGCPHCGMAVTIPPLSAPSEE
                     PPGQPPSFEPPREFGLPVQQPAWSDREPPSVQPSRAAPALATAASDRFPPGMSPPAEA
                     KKETADARRTEAKSKPSAPSSREATDGLPPKPARPALADLLPPGAAAVPPSAGAKKPA
                     FEPPSARQEPKPAALQPEPPADETSSAAPLESLLPPGAAEAPVSAPLLSPRPSDAETL
                     LPPGAAPASSAPEAGEQVALPKAPKKAPVAPANVPAGTIAVPTPDGGFVTVRETPKII
                     GHGEEEIEVRRLTPQEKEQRRFRRNLILGVICLAIILAVFAALVWR"
     gene            complement(1419..1892)
                     /locus_tag="VFV87_05805"
     CDS             complement(1419..1892)
                     /locus_tag="VFV87_05805"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX5103303.1"
                     /translation="MLSQDYQPARLFTIEQANAMLPLVRAITTDLARLGSDVMERRHR
                     LALVAEGRKLKPGDPYSDELAHMRDELEKDQIKLREYVEELQELGVEPKGAIEGLVDF
                     PCLMDGRVVYLCWKLGEPEVLYWHDLESGFAGRQSLTADSVSGDFGDDASESLDG"
     gene            complement(2035..2562)
                     /locus_tag="VFV87_05810"
     CDS             complement(2035..2562)
                     /locus_tag="VFV87_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5103304.1"
                     /translation="MWQWLRNIWLAIYTVGHGMYVTLRYWLITYRPDRGAFTQQFEYP
                     EKPVPVAARYRGFHRYDLTTCIACDQCAKACPVDCIYIGKERVENGKGFKITGFTIDY
                     TKCMFCALCVEPCPVDCIFMGATHDLSCYSRDGCIVDFSRLPVEVAWGKSTLNPTAVA
                     ESKVIAQPVHGGPNQ"
     gene            3161..3376
                     /gene="csrA"
                     /locus_tag="VFV87_05815"
     CDS             3161..3376
                     /gene="csrA"
                     /locus_tag="VFV87_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX5103305.1"
                     /translation="MLVLSRKKNESIVINNDITIVVVEIRGDKVRLGVEAPKEVPVHR
                     REVYDAIKRNAAVGGDSTSDASAAAQS"
     gene            3649..3721
                     /locus_tag="VFV87_05820"
     tRNA            3649..3721
                     /locus_tag="VFV87_05820"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3683..3685,aa:Glu,seq:ttc)
     gene            3845..>4038
                     /locus_tag="VFV87_05825"
     CDS             3845..>4038
                     /locus_tag="VFV87_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103306.1"
                     /translation="MNRHQVIVALILLVVAARAPEAAYAESPELRWVYLQQNLQVKEN
                     LPKIEEILRRAKAAGSNGVVL"
BASE COUNT          748 a         1319 c         1197 g          774 t
ORIGIN      
        1 ccactccttc gttcgccagt accgcgaccc caccatcaaa cgctcctact tccaaaccgc
       61 tcaactcaaa ctctagccag gcgcatttac ggcgcagggt tcaataatgt tatccagatg
      121 gagcgccgtg accgcaattg actcccgacc ccttccgctg atctagcgcc agaccagggc
      181 ggcgaagacc gccagaatga tcgccaggca gatcaccccg aggatcagat tccggcgaaa
      241 ccgtcgctgt tccttctcct gcggcgtcag ccggcgaact tcgatctctt cttcgccgtg
      301 cccaataatc ttgggcgtct cgcggacggt gacaaagccg ccgtcgggcg tgggaacggc
      361 gatcgtccct gccggcacgt tggccggtgc aacgggcgct ttcttgggcg ctttgggcag
      421 cgcgacctgc tcgccggctt cgggggccga gctggcgggt gccgcgccag gcggcaaaag
      481 cgtttcggcg tccgacggcc gcggggaaag caggggagcg gacaccggcg cctcggcagc
      541 gcccggcggc agaagcgatt cgagcggcgc agccgacgaa gtttcgtccg ctggcggctc
      601 tggctgaagc gcggctggtt tcggctcctg tcgggccgaa ggtggttcga aggccggctt
      661 cttggccccg gcggacggcg gcacggctgc tgccccgggc ggcagcaaat cagcgagtgc
      721 cggccgcgcg ggtttcggtg gcaggccgtc ggttgcttcg cggctggatg gcgcggacgg
      781 cttcgatttg gcttcggtgc gccgcgcgtc cgccgtttcc ttcttcgctt ctgccggcgg
      841 cgacatcccc ggcggaaagc gatcgctggc agcggttgcc agtgcgggcg cagctcggct
      901 cggttgcacg ctcggcggct cgcgatcgct ccaagccggt tgctggacgg ggagaccgaa
      961 ctcgcgcggc ggctcaaagc tcggcggctg gcccggcggt tcctcacttg gcgcggacag
     1021 cggcgggatc gtcaccgcca ttccgcagtg cggacagcca agctgctgac cgctcatcga
     1081 ggccagcact tgaaatggcc ccgcacaagc cggacaggca agcgtgaaca gctcgtccgc
     1141 gggcggtggc ggaggcggcg tctcggctgc cgccaagagc cgcatcagca catcttccgg
     1201 cggaacgacg aacggcccct ggcaataagg acagccaagt tgctgcccgc ccgtcgccga
     1261 gtcaacctga atcaggttcg cacaatgcgg acattgaagg ggcacaaccg gcataaggcg
     1321 gagcaagcga atcggcagag gcgggcaaaa cggctatcgt agccgcacgt cctgcaagcc
     1381 actagcggca agtggcgctc gaacaatcgc gaggcccgtc agccgtcgag cgactcgctc
     1441 gcatcgtcgc caaagtcccc gctgaccgaa tctgccgtca gcgattgccg gccggcaaag
     1501 cccgattcga gatcatgcca gtagagaacc tccggctcgc cgagcttcca gcagagataa
     1561 acgacgcgcc cgtccatcaa gcacggaaaa tcgaccagcc cctcgatcgc tcctttgggc
     1621 tccacgccaa gttcctgcag ctcctcgacg tactcccgca gcttgatctg gtccttctcc
     1681 agctcatccc gcatgtgggc cagctcatcg gaatatggat cgccgggctt gagctttcga
     1741 ccttcggcca ccagcgccag ccggtgccgt cgttccatca catcacttcc caggcgggcc
     1801 agatcggtgg tgatggcccg caccagcggt agcatggcgt tggcctgttc gatcgtgaag
     1861 agcctggccg gttgataatc ttgggacaac atcggatgcc acctcaacga acgaatgccc
     1921 cgtttggctc atttccgtcc tgcacgaacc accgctgcta tcgagcagat tcggctgctc
     1981 cctttcattg tattcccagg gacgattctt gccacccttt ggcgctcact gatgctactg
     2041 attcggccct ccgtgaaccg gctgggcaat caccttcgat tcggccaccg ccgtcggatt
     2101 cagcgtcgat tttccccacg ccacttccac cggtaaacgc gaaaaatcga cgatgcagcc
     2161 atcgcggctg tagcacgaca gatcgtgcgt ggcgcccatg aagatgcaat cgaccgggca
     2221 cggctccacg cagagtgcac aaaacatgca cttcgtgtag tcgatcgtga agcccgtgat
     2281 cttgaacccc ttgccgttct ccacccgctc cttgccgatg taaatgcaat ccaccgggca
     2341 ggccttcgca cactgatcgc aggcgatgca cgtcgtcagg tcgtagcggt gaaacccgcg
     2401 atagcgggcc gccacaggca ccggcttctc cgggtactca aactgctgcg tgaacgctcc
     2461 gcgatcgggc cgatacgtga tcagccagta ccgcagcgtc acatacatcc cgtgtccaac
     2521 ggtgtatatc gcgagccaaa tgttgcgaag ccactgccac atggtgcggg tctaaccttg
     2581 tccggctgcc agggcgcgcc gccgaaagcg ttccagtcaa gcgattctgc caatctcacg
     2641 ctgaattata gggaggcgcg ctgcacccgc aaggacgcgg cgcaacagtc gttgcggcgg
     2701 tcgaactctc ctcgtaacgc gcaactgcaa tctcgtttaa cgtagagccg caggcgtagc
     2761 tgaatccggc ctcgatttcc ctggcaaccc agcgtcgccc gattaaatcc ccatcgaaac
     2821 gccgcttggc gatggcacca gaagattcaa tcgataaaac cacctcaaat atgtgtattt
     2881 attcatctta cgttgtgtat atacctgcgt atgggatgaa tgctccgtct gtgccggttg
     2941 atctgccccg ctcccgcagg atttcgcaaa ttcctgcccc cttgtggcgg agaattcttg
     3001 actcgcccca atcacagttc acaatgaatc gtaggctaga agtgccacat tgccggcttg
     3061 ccgggccatg ggggaatact gcgctacagc aactcctgcc cgtttttgca gccgcaatcg
     3121 ccgatggaag tggcccttcg cccaggaaag gattgtcgtc atgttggtgc tctcccggaa
     3181 aaagaacgaa agcatcgtca tcaacaacga catcacgatc gtcgtggtgg aaatccgcgg
     3241 cgataaggtg cggctcggcg tcgaagctcc caaggaagtg ccggtccacc gccgtgaggt
     3301 ctatgatgcg atcaagcgca acgctgccgt aggcggcgat tcgacttcgg acgccagcgc
     3361 ggcggcccag tcgtagttgt gccttcgcgt tgacagcttc gttccccaca aaaccacctc
     3421 cattctcagc gtgctgcgac cgctttagcc acgccagggt cgcacttttc cgccggccgg
     3481 ttcgcatctc gccaccgcga accccacttg acgctggccg ctctaagccc ttagatggct
     3541 tgagaatcaa gctccaagaa ccgaagatcc aagaaccaaa aggtggtgag tccggtcgca
     3601 gttcttggac gttggttctt cagctcttgg ttctttggtc atccccgcgg ccccatcgtc
     3661 tagcggtcta ggacaccgcc ctttcacggc ggtaacaggg gttcgagtcc ccttggggtc
     3721 acttttcgtc cgcgcgtcat cgccttcttc gtgcaggcgc gcggtctttg taccttcccc
     3781 tttgcacggg tggtcgtgtc cgtggacgcg cggtcgcgcc tcaaggattt cacggtacga
     3841 tgcaatgaac cggcatcagg tcatcgttgc gctcatactg cttgttgtcg cggcccgcgc
     3901 gccggaggcg gcctatgccg agtcacccga gctccgctgg gtctatctgc agcagaacct
     3961 gcaggtgaag gagaatctgc cgaagatcga ggaaatcctg cggcgggcga aggcggccgg
     4021 ctctaacggc gtcgtact
//
LOCUS       DASUGB010000304         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_173266, whole genome shotgun sequence.
ACCESSION   DASUGB010000304 DASUGB010000000
VERSION     DASUGB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173266"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /gene="flgN"
                     /locus_tag="VFV87_05830"
     CDS             <1..383
                     /gene="flgN"
                     /locus_tag="VFV87_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HEX5103307.1"
                     /translation="AVDLPGLAALQPREEELVERLEKCQQRRKELLAAAAASGRPSGS
                     IGKLAESFTQPQRGKLGRQVKEASARLRLLQHQSLTNWVLAQRSLLHAAQLLEIIATG
                     GRIQPTYGEQGSVPARSSLVNHEA"
     gene            411..2108
                     /gene="flgK"
                     /locus_tag="VFV87_05835"
     CDS             411..2108
                     /gene="flgK"
                     /locus_tag="VFV87_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX5103308.1"
                     /translation="MTLFSSIQTAKNSLLVAQLGLQVTGNNIANANTPGYIRERLILN
                     PQPPQRYGGHLVGLGVDAEGVVQVIDRFLEERLRRANSDLAGGEALEHAYVQLEGLIG
                     ELSDTDLSTSLTDFFASIHDVLNQPESVSVRNLAVLEGQALTDDIRRLDSQAREIHQD
                     LNGRIISTADRINALLEDIAKLNGQIVEAENSDVSASDAVGLRDKRSKVLAELASIVD
                     IRTVEQEAGDVTVFSGGDYLVAGVNWRPVKVVHSTENGLSVAEIRITELDAPLVTTSG
                     ELAGLLAARDTVLGGFIEDLNSFTRTLLFEFNKVYAGGQGLAGYSSATSEFAVSDVAA
                     ALNQAGLEFSPVHGGFQILVKNEQTGLTKTTDIQIDLDGLATDTSLTDLAAQLDAVDG
                     IAAIVTPEGKLQIASDSNDVSFAFANDTSGILAALGINTFFSGSSASDIGINAALRAN
                     PRLFAASAGGIGEDSANATRLATLLTEPLASHNGDSLAILYDRLTGDVAQGAAGARAT
                     TEGYRVFQRTLEGQHLAITGVNIDEEAVQMIAWQRAFQASAKVISTINELLETLVNL"
     gene            2120..>3575
                     /locus_tag="VFV87_05840"
     CDS             2120..>3575
                     /locus_tag="VFV87_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103309.1"
                     /translation="MALYPVPTTRASGQLIQNRLLTQLHSDQLALLRLQQQISTGYRI
                     TSPSEDAPAAGRAQVLQRLLELKAQAQVNQQTSQSYLDASDVAINGLTKLLSDIRATA
                     LGVADTVSTDTQRQAAAQEVLEAIKQLGTMGNQNFRGRYLFAGSRADTIPFEFIGDYV
                     AFRGNEGELSSYVDLNLLYPTNVPGSEVFGTFSPGVRSTVDFNPALTPGTPLSALRGG
                     QGISPGSIVISDGTNSQTIDLSGAATIGDVAALLAANPPTGRTITATVTATGLTVDID
                     DAGGGNLTIREVGNGTTAFELGILNTTGTGVAPIVGGDLNPRLRLTTLLSDVLGGTLD
                     QASGIQIVNGGQTYTITFGSAVTVEDLLNEINGSGANVLAEIDPAGDRIAIRSRLSGS
                     DFAIGENGGTTAAQLGVRSLTLQTALADLNHGIGIHEEPGTDFTIRRRDGVLLDIDVG
                     GATTIGDILNLINTHPANVGPDAVVAQLAASGNGI"
BASE COUNT          680 a         1183 c         1129 g          583 t
ORIGIN      
        1 ctgccgtcga tttgcccggc ctggccgccc tgcagccgag ggaagaggag ctcgtcgagc
       61 gcctggaaaa gtgccaacag cgccgcaaag agctgctcgc ggcagcggcg gccagtggcc
      121 ggccgagcgg cagcatcggc aagctggcgg aatccttcac ccagccgcag cgcggcaagc
      181 tgggccggca ggtcaaagag gccagcgccc gcctgcggct cctgcagcac cagagcctga
      241 cgaactgggt gctagcacag cgaagcttgc tgcatgcggc gcaacttctg gaaattatcg
      301 ccaccggcgg aagaatccag ccgacttatg gagagcaggg gtcggtgcct gcgcgctctt
      361 cgctcgtgaa tcacgaggcc tagcgccgcc ccacctcgtg ggcgcggcgc atgactctct
      421 tcagctcgat ccagacggcg aaaaactcgc tgctcgtcgc gcagcttggc ctgcaggtca
      481 ccggcaacaa catcgctaat gcgaatacgc cgggctacat ccgcgagcgg ctgattctca
      541 acccgcagcc gccgcagcgc tacggcggcc acctggtcgg gctcggcgtc gatgcggaag
      601 gggtcgtgca ggtcatcgac cgcttcctgg aggaacggct tcgccgcgcc aatagcgatc
      661 tggccggcgg cgaagccctg gaacacgcct acgtccagct cgaagggttg atcggtgaac
      721 tgagcgacac cgacctcagc acctcgctga ccgatttttt tgccagcatc cacgacgtgc
      781 tcaatcagcc cgagagcgtc agcgtccgca acctggctgt gctggaaggc caggcactga
      841 cggacgacat ccgccgcctc gatagccagg cgcgcgaaat ccaccaggac ctcaacggcc
      901 ggatcatcag tacggcggac cggatcaacg cgcttttgga agacatcgcc aagctcaatg
      961 ggcagatcgt cgaagcggaa aacagcgacg tttcggccag cgacgccgtc ggtctgcgcg
     1021 acaagcggag caaggtcctg gcggagctcg cgtcgatcgt cgatatccgc accgtcgagc
     1081 aggaggcggg cgacgtaacc gttttctcgg gaggggacta tctggttgcc ggcgtcaact
     1141 ggcggccggt gaaagttgtc cactcgacgg agaacggctt gtccgtcgcc gaaatccgca
     1201 tcaccgagct cgatgcgccg ctagtcacca cgtccggcga gctggctggc ctgttggctg
     1261 cccgcgatac cgtcctgggc ggcttcattg aggacttgaa cagcttcaca cgcacgctgc
     1321 tgttcgagtt caacaaggtt tacgccggtg gccaggggct ggctggatac tcgtccgcga
     1381 ccagcgaatt tgccgtcagc gacgtcgcgg cggcgttgaa ccaggccgga ttggaattct
     1441 cgcccgtcca cggcgggttt cagatcctgg tgaagaacga gcagacgggc ctgacgaaaa
     1501 cgaccgacat tcagatcgat ctggacggct tggcgactga cacatcgctg accgatctgg
     1561 ccgcgcaact tgacgccgtc gatgggatcg cagcgattgt cacccccgaa gggaagctgc
     1621 agatcgcgag cgattccaat gacgtctcgt ttgcgtttgc caacgacacg agtggcatcc
     1681 tcgcggctct tggaatcaac acgttcttct cgggcagctc ggcgagcgat atcggcatta
     1741 acgcggcgct gcgggccaat ccgcgtttgt ttgcggcttc ggccggcgga atcggcgaag
     1801 attcggccaa tgccacgcgc ctggccacgc tgctcaccga gccgctcgcc tcgcacaacg
     1861 gcgactcgct cgccatcctg tacgaccgcc tgaccggcga cgtggcccag ggcgccgcgg
     1921 gagctcgcgc gacgaccgaa ggctaccgcg tctttcagcg gacgctggaa gggcagcacc
     1981 tggccatcac gggcgtcaat atcgacgaag aagcggtcca gatgatcgcc tggcagcggg
     2041 cgttccaggc gtcggcgaag gtgatttcga ccatcaacga gctgctggaa acgctggtca
     2101 acctgtaagt ccacccgcca tggcccttta tcccgtcccg acaacccgcg ccagcggcca
     2161 gctcatccag aaccgtctgc tgacgcagct ccacagcgat cagcttgccc tgctgcgcct
     2221 ccagcagcaa atcagcaccg gctaccgaat cacgtcaccc agcgaagacg ccccggcagc
     2281 cggacgggcc caggttttgc agcgtctgct ggagctgaag gcccaggcgc aggtcaacca
     2341 gcagacgagc cagtcgtatc tcgacgccag cgacgtcgcc atcaacggcc tgacgaagct
     2401 gctttccgat attcgcgcga ccgcgctcgg cgtggcggac accgtcagca ccgatacgca
     2461 gcggcaggct gcggcgcaag aggtgctgga ggccatcaaa cagctaggaa cgatgggcaa
     2521 ccagaatttc cgtggccggt acctcttcgc cggatcgcgg gccgacacga ttcccttcga
     2581 gttcatcggc gactacgtgg ctttccgcgg caatgagggc gagctgtcga gctacgtcga
     2641 tctgaacctg ctctatccca ccaacgtgcc gggaagtgaa gtcttcggga ctttttcgcc
     2701 cggcgtccgc agcacggtcg atttcaaccc ggcgctcacg ccgggtacgc cgctttcggc
     2761 cttgcgcggc ggacaaggca tctcgccggg cagcatcgtc atcagcgatg gcacgaattc
     2821 ccagacgatc gatctgagcg gcgcggcgac gatcggcgac gtggcggcgc tgctcgcggc
     2881 gaatcctccg accggccgca cgatcaccgc gaccgtcacc gcgacggggc tgacggtcga
     2941 tatcgacgac gcgggaggtg ggaacctgac gatccgcgag gtcggcaacg ggacgaccgc
     3001 tttcgagctg gggattctca ataccacagg aacgggggtt gcgccaattg tcggcggcga
     3061 cctgaacccg cgtttgcgtc taacgacgct cctcagcgac gtgctgggcg gcacgctcga
     3121 ccaggcctcg ggaatccaaa tcgtcaatgg cggccagacc tacacgatca cctttggctc
     3181 tgccgtcacc gtcgaggacc tgctgaacga gatcaacggc tcgggggcga acgttctggc
     3241 cgaaatcgac ccggcaggcg accgaatcgc gatccgatcg cgcctcagcg ggtcggattt
     3301 tgcgatcggc gagaatggtg gtaccacggc tgcgcaactc ggtgttcgct cgttgaccct
     3361 gcagacggca ttggccgacc tcaatcacgg catcggcatc cacgaggagc cgggaaccga
     3421 ctttacgatc cgtcgccgcg acggcgtgct gctcgatatc gacgtgggcg gagcgacgac
     3481 gatcggcgac atcctcaatc tcatcaatac ccaccccgcc aacgtcggcc ccgacgccgt
     3541 cgtcgcccag cttgctgcca gcggcaacgg catcg
//
LOCUS       DASUGB010000305         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_312360, whole genome shotgun sequence.
ACCESSION   DASUGB010000305 DASUGB010000000
VERSION     DASUGB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312360"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFV87_05845"
     CDS             complement(<1..319)
                     /locus_tag="VFV87_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX5103310.1"
                     /translation="MPATCPPERYCPQRELPPYGYVPGQGLPHPTSDKRGHSFGRHEP
                     PAMPLADATWRQNETWLYAVDLFNQGYYWEAHEAWEALWHACGRKGPTADLLKGLIKL
                     AAAG"
     gene            383..1456
                     /locus_tag="VFV87_05850"
     CDS             383..1456
                     /locus_tag="VFV87_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX5103311.1"
                     /translation="MTHALPSLQLGPVHLGFPVVQAALSGYSDWPMRLIARRHGASYT
                     LCEVMLDQFLVAIKDNRQRNRHFLHLTDDERPVGGQLMGAEPEQFAAGAARLAQAGFD
                     VVDINFGCPVKKVLGRCRGGFHLSQPAVALEIVRRTRDAVPPHIPVTVKMRRGIDDTP
                     ESRDKFFTILDGAFAAGIAAITVHGRTVVQRYIGPSRWEFLSEVKRHVGPERTILGSG
                     DLFSPHDCLEIMRQTGVDGVTVARGAIGNPWIFAQARALAAGQPLPPPPTLWQQRDAM
                     LEHFRLAEQLYGVGRAGPHMRKIGIKYAASHPQHLLVREAFTRVHSLDDWQAVLAHWY
                     ASDLAGCYPDPALHRTGNCEEAA"
     gene            complement(1492..>2527)
                     /locus_tag="VFV87_05855"
     CDS             complement(1492..>2527)
                     /locus_tag="VFV87_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103312.1"
                     /translation="PYRAVGNAVPGVPETDNDIPRNLCLPFRFSSKSPQFTRGGPYGG
                     FLGRGFDPICTEFDGPITGKIERWTGSAMAKVDEPYLGVTADGKFVISKAGQLGNDLT
                     AQRVDSRRSLLAQFDQARRELDESPSARGHDRFREMAYSLLTSPAIRAALDISRETPE
                     KRAAYGLNVFGQATLAGRRLLEAGATLVTVIWDEITIANSAWDTHFHHYERLADELLP
                     GLDRALSSLILDLEDRGLLDDTLVLCLTEHGRTPKLHDKGAQGAGREHWSDVYSNVLA
                     GAGIAGGAVIGSSDKHGAYVQDNPISPKDILCTLYHLLGIDPHRTIPDRFGRPFPLVS
                     EGRVIAEMLG"
BASE COUNT          402 a          902 c          802 g          421 t
ORIGIN      
        1 cccccgcggc tgccagcttg atcagcccct tcagcaaatc ggcggtcggc cccttgcgcc
       61 cgcaggcgtg ccagagcgcc tcccacgctt cgtgggcctc ccaataatag ccctggttga
      121 acagatcgac cgcataaagc cacgtctcat tctgccgcca ggtcgcatca gctagcggca
      181 tcgcgggcgg ctcgtgccgg ccaaagctgt gcccccgctt gtcgctggtc ggatgcggca
      241 ggccctggcc aggaacgtag ccatagggcg gcagctcgcg ctgcggacag tatcgctcgg
      301 gcggacaagt tgccggcata tcaaacctca aatcgagggc ctgccggtac aatactggca
      361 gaatcgccct tagcaacagc ctatgaccca cgctctccct tccctgcaac tcggccccgt
      421 tcacctcggc tttcccgtcg tgcaggccgc cctctccggc tatagtgact ggccgatgcg
      481 cctgatcgcg cgacggcacg gggcgagcta tacgctgtgc gaggtcatgc tcgaccagtt
      541 cctggtcgcg atcaaggaca accgtcagcg gaaccgccat ttcctccatc tgaccgacga
      601 cgagcgcccc gtcggcggcc agctcatggg ggccgagcca gagcagttcg ccgccggcgc
      661 cgcccggctc gcccaggcgg gcttcgacgt cgtcgatatc aacttcggct gcccggtgaa
      721 aaaagtcctc ggccgctgcc ggggcggatt tcacctcagc cagccggccg tggctctgga
      781 aatcgtccgc cgcacgcgcg atgccgtccc gccgcacatt ccggtcaccg tgaagatgcg
      841 ccgcggcatc gacgacacgc ccgaaagccg cgataagttc tttaccattc tcgacggcgc
      901 ctttgccgcg ggcatcgccg ccatcaccgt ccacggccgc accgtggtgc agcgctacat
      961 cggccccagc cgctgggagt tcctgtccga agtgaaacgc cacgttggcc cggagcggac
     1021 gatcctcggc agcggcgatc tcttttcgcc gcacgattgc ctggagataa tgcgtcaaac
     1081 gggtgtcgat ggcgtcaccg tggcccgcgg cgcgatcggc aacccgtgga tcttcgccca
     1141 ggcccgcgcc ctggccgccg gtcagccgct cccgccgccg cccacgctct ggcagcagcg
     1201 ggacgcgatg ctcgagcact tccgcctggc cgagcagctc tatggcgtcg gccgcgccgg
     1261 cccgcacatg cgcaagatcg gcatcaaata cgccgcctcc catccccagc acctcctcgt
     1321 ccgcgaagcc ttcactcgcg tccactccct ggacgactgg caagccgtcc tcgcccactg
     1381 gtacgcctcc gacctggccg gctgctaccc cgaccccgcc ctccaccgca ccggcaactg
     1441 cgaagaagcg gcctaagtgg ggcagacatt cctgtctgcc acattccctc tctacccgag
     1501 catctccgca atcacccttc cctcactcac cagcgggaac ggccggccga accggtcggg
     1561 gatcgtgcgg tgggggtcga tgccgagcag atggtagagc gtgcagagaa tgtccttggg
     1621 gctaattggg ttgtcctgca cgtaggcccc gtgcttgtcc gagctgccga tgaccgcccc
     1681 gcctgcaatc cccgcgccgg ccagcacgtt gctatacacg tccgaccagt gctcgcggcc
     1741 ggcgccttgc gcccccttgt cgtgcagctt cggcgtgcgg ccgtgctcgg tcaggcagag
     1801 gacgagcgtg tcgtcgagca ggccgcggtc ttccaggtcg agaatcagcg acgacagcgc
     1861 ccggtcgagc cccggcagca gttcatcggc cagccgctcg tagtgatgga agtgcgtatc
     1921 ccaggcggag ttggcgatcg tgatttcgtc ccagatgacc gtcaccagcg tcgcccccgc
     1981 ctccaagagc cgccggccgg cgagcgtcgc ctggccgaag acgttcaggc cgtaggctgc
     2041 gcgcttctcg ggcgtttcgc ggctgatgtc gagggcggcg cggatggcgg gcgaggtgag
     2101 cagcgaatag gccatctcgc gaaagcggtc gtggccgcgg gctgaagggg attcgtcgag
     2161 ctcgcggcgg gcctggtcga actgggccag cagcgacctc cggctatcga cgcgctgggc
     2221 tgtcagatcg ttgcccaact gcccggcctt ggaaatcacg aatttgccgt ccgcggtgac
     2281 gcccaggtac ggctcatcga ccttggccat cgcactgccg gtccagcgct cgatcttgcc
     2341 ggtgatcggg ccgtcgaact cggtgcaaat gggatcgaag ccgcggccga ggaacccgcc
     2401 atatggcccg ccgcgggtga actgcgggct cttgctgctg aagcgaaagg ggaggcagag
     2461 gttgcgggga atgtcgttgt cggtctccgg aacgccaggg acggcgttcc ctacagcgcg
     2521 gtaggga
//
LOCUS       DASUGB010000306         9414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1563873, whole genome shotgun sequence.
ACCESSION   DASUGB010000306 DASUGB010000000
VERSION     DASUGB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563873"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2482)
                     /locus_tag="VFV87_05860"
     CDS             complement(<1..2482)
                     /locus_tag="VFV87_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103313.1"
                     /translation="MSSRSSAVPAVILGFLASLSAAADEPKPEFDPAHVEKIQEGLRL
                     FKSQVRQVLIDNCVDCHGGAEVESGLDLATRKGLLRGGSHGPAVITGKSIDSNVIRFI
                     SHREKPFMPEGSDKLPDEQIAAISRWIDLGAPYDQPLVENPRDPDSWTATVVPAKARE
                     FWSFQPLAHPQPPAVRNEAWVRNDLDRFVLAKLEEKGLTPNSPAERRVLIRRAYFDLV
                     GLPPTPEEVEKFVADIDPRAYEKLLDTLLESPHFGERWARHWLDAVRFAESHGFEQDY
                     DRPFAYHFRDFVIEAFNQDLPYDRFVRWQLAGDEFEPANPLALKATGFLGAGVFPTQI
                     TANEVERTRYDALDDMAATTGSAMLSLSIGCARCHDHKFDPIPQADYYRFLSTFTTTV
                     RSNLDVDVTPEKTKAALAAWEKEHQPKAAALQTYEETAMPGEFQKWLDESPQLDGKWR
                     KDVEWIVFDKAEAKSKNGATLTAQADGSILVSDKNPDNDTYTITTRPFDQLGTAIKTI
                     RLEALPDASLVKSGPGRADNGNFALSRIRMFAEEADGSQRREIKLTNPRADFQQNDGN
                     LSIAAALDDDPKTGWAVDPQFGQKHVAVFDVDESTPIGSWVSLRLVFELDFAVNTKHT
                     LGKLRLSRDYQGLKEPLAKQGVSRTQSVAQALNAIDGSGSALLPGLEEHQRATLLSEF
                     KRQSARWNELNDAVQKSLAARPQPNLVKMMVCSEGVTPIRHHTQGADFFDETYFLKRG
                     DCDQKMGVASQSFLQVLMTAPEGQSHWQLAPPEGSKISYRRRALANWITDTEYGAGHL
                     LARSIVNRLWHHHFGRGIVSTLNDFGVQG"
     gene            2740..3405
                     /locus_tag="VFV87_05865"
     CDS             2740..3405
                     /locus_tag="VFV87_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103314.1"
                     /translation="MLFRSALLAIVLSVAGCNMQPMKVCVYIDNSGHEPMLITVDGQE
                     EMTVPSGEVAELKLPPGEHQFLIRAGSETVCDLMRNLEPSQRFGVARKYLFDPYKNHR
                     YQTYYAQYGESRLGDLMESTLFSFQTDPDAQLQYVYKQLLKEIDLVPSDAWNDVTGVD
                     YVLTAPPDEIYTSSMEKRRVLDRIEPAFYEQFLEAKQVQNPTDEDVEKLGDLLDEVLA
                     QSL"
     gene            3556..3759
                     /locus_tag="VFV87_05870"
     CDS             3556..3759
                     /locus_tag="VFV87_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX5103315.1"
                     /translation="MLVLSRKVGERILIGDQIAITVVRINATTVRLGIDAPSKLAVVR
                     EELAEGSPPSSAPSGDPAKLAAR"
     gene            complement(3775..4839)
                     /locus_tag="VFV87_05875"
     CDS             complement(3775..4839)
                     /locus_tag="VFV87_05875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5103316.1"
                     /translation="MVTPSAQPPSGQSASRKLQSAIDLSAEPLELIVPPADAGLRLDA
                     FLARSLPKYSRVQLRKVIGSGGVTVNEQGTKVAYKLSAGDRVRVVLPSMNTAGPHPEN
                     IPLDILYEDEHIIAVNKPPGMVVHPARGHWSGTLASALSFHFQQLSKAGGPTRPGIVH
                     RLDRDTSGVMVVAKTDPMHYVLAAQFEARTTEKEYLAITAGVPDRDRDIIQQPIGIHP
                     HQREKMAIRAGHSTSRDASTFYEVVERFPGYALVRVLPKTGRTHQIRVHLAHIGCPVL
                     CDKLYGGRSQITRGEVQQYGTAGGARSLAANATDVVLSRQALHARRIKLSHPATKEPI
                     EFVAPLPGDLSVVLSTLRSA"
     gene            complement(4887..5831)
                     /locus_tag="VFV87_05880"
     CDS             complement(4887..5831)
                     /locus_tag="VFV87_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103317.1"
                     /translation="MVVKSHRQLRHEREHNELADWLTERIDVLKPYGTHITVAVIVVL
                     LAVIGGIYYFGTESAATAKEWADYRAALNERDPEKSLQNLLDDKPSSVVGLWAAQTLG
                     DINVARGAGLMFSDREQAKDLLEKARGYYEKAAAAPSDPDLVARARMGLGKVYESLCQ
                     PEEALKYYKQVADSRPDSAIGKAAASDVKRMENPRTVALLAWFAEQKPTKPSPLPGAG
                     GGLPGLPNDLPDRPDISLPGGTLGLDDIGTGVPASPEPTLPQPGTTQPEPKPEVPTTE
                     EPKPADANTGEARLEEDKPAQAKPAEETANPTKEDKPE"
     gene            complement(5961..6728)
                     /locus_tag="VFV87_05885"
     CDS             complement(5961..6728)
                     /locus_tag="VFV87_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5103318.1"
                     /translation="MHRNLLLPIWPLRRLLVCFVIVAIAMGGRLAAQEAPENPAAPKS
                     VRTPPALKAYKGRQIAQTMHYLGAEWLTRDNREQEERCSLMLANLGLKRGMAICDMGC
                     GNGFYALQMAKMTGDDGHVYGVDIQPEMLKFLNERADEQGIRNVSPILGTFTDPRLPK
                     GKIDLILLVDVYHEFSHPEQMLAAMREALSPTGACVLVEFRTEDPKVPIKPEHKMSKA
                     QIMKEWPANGFKLVKEFDGLPWQHMMWFARDDQTAEK"
     gene            6796..7329
                     /locus_tag="VFV87_05890"
     CDS             6796..7329
                     /locus_tag="VFV87_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEX5103319.1"
                     /translation="MAERAGNLVRYPERIVRFQMQQKWLSWGDSFVIQDEEGREVLYV
                     QGAVFSWGNQLSIREPGGLEVAEVKQIVFSWGPTYEIYRNAALTARVTKELFTFFHCR
                     FAIDVPGPDDLEAEGDFWQHEYAIRRGAQIVATVSKQWFAWTDTYGVEVAPGEDAHLV
                     LACAVVIDCCCHDKRND"
     gene            complement(7361..8350)
                     /locus_tag="VFV87_05895"
     CDS             complement(7361..8350)
                     /locus_tag="VFV87_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103320.1"
                     /translation="MDCFLAHGALAMAMGLRTALAFTMMVLVASLRCAGQEAAGAEEG
                     AETLQLLKSYYRHKAQQYEFTLDAEGKQKLVLEPVAMTYTGHDYQSVANTVSANSGEV
                     YIWTYEGRAVIAGGIGSFPVQGRWDFFHEFHVLTQRAPQPTKINSLPDLVWRPEGETP
                     KLIPGAPAPASAESTPLARSRRLTQMRSLAHEFSGTTVKPDDSGKARLRLLPTPIYRL
                     DSAELEKGQHKIIDGAVFVFTGQDGTDAEMLLSIECRKSDSGLEWTYVPAAMTYLEMW
                     MEHKGTEVWHIPNYRTDQRQHNYFTEIVERITSLDEMRTRLNSADAAAREQSR"
     gene            8550..8834
                     /locus_tag="VFV87_05900"
     CDS             8550..8834
                     /locus_tag="VFV87_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103321.1"
                     /translation="MILFSKLPAANWWTCLTVVACAAILLGNAIYFDGGPPPRFLLEK
                     GEWARNPWWLAAFYFHIVGASVSLAAGTPLMFPPGRENILRGIAAWGSSM"
     gene            8831..9223
                     /locus_tag="VFV87_05905"
     CDS             8831..9223
                     /locus_tag="VFV87_05905"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX5103322.1"
                     /translation="MNAVLWMAAPAGLALALVAKGGLWGTAGFALAGAFWWQTTWSGY
                     LAIRRGDVAGHIRAMVRSYCWALSAPAFRVIQAALFLAGFADGINYVLSLWLSIAASV
                     WLAESFLFRDRRKQLEVLPSSPSAGVLT"
     gene            9220..>9414
                     /locus_tag="VFV87_05910"
     CDS             9220..>9414
                     /locus_tag="VFV87_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103323.1"
                     /translation="MNRSLIVPACCSLLAFTALLAALHGVLARETGQPQTAGGQANRE
                     RNPTELAYSSVAVPRLQGERL"
BASE COUNT         1590 a         2863 c         3061 g         1900 t
ORIGIN      
        1 cgccctggac gccgaaatcg ttaagcgtgc tcacgatgcc gcggccgaag tggtgatgcc
       61 acaggcggtt gacgatcgac cgggcgagca ggtggcccgc gccgtattcc gtgtcggtga
      121 tccagttggc cagggcgcgc cggcgataac tgatctttga cccttcgggt ggcgcaagct
      181 gccaatgcga ctggccctcc ggcgcggtca tcagcacctg caggaagctc tgcgaggcca
      241 cacccatttt ttgatcgcag tcgccgcgct tcaggaagta cgtctcgtca aaaaagtcgg
      301 cgccctgcgt atgatgccgg attggagtca ccccttcgct gcagaccatc atctttacca
      361 ggttcggctg cggcctggcg gcgaggctct tctgcaccgc gtcgttcagc tcgttccacc
      421 gcgcgctttg acgtttgaat tcgctcagca gggtcgctct ttgatgttcc tcgagccccg
      481 gcaacagcgc cgacccgctg ccgtcgatcg cattcagagc ctgggccaca ctctgcgtac
      541 gcgatacccc ttgcttcgcc agcggctcct tgagtccctg atagtcgcgc gatagccgca
      601 gcttgccgag cgtgtgcttg gtgttcacgg cgaagtccag ctcgaaaaca agccgcaggc
      661 tcacccagga accgatcggc gtcgattcat ctacgtcgaa tacggcgacg tgtttctggc
      721 cgaattgtgg atcgacagcc cagccagtct tcggatcgtc gtcgagcgcg gcggcgatcg
      781 agaggttgcc atcattttgc tgaaagtcgg cccgcggatt agtgagtttg atttcgcgcc
      841 gctgcgagcc gtcggcctcc tccgcaaaca tccggatgcg gctgagggcg aagttgccgt
      901 tgtcggcgcg gccggggccg ctcttcacca aggatgcgtc aggcagggcc tcgagccgga
      961 tcgtcttgat cgctgtgcca agctgatcga agggtcgcgt cgtgatcgtg taggtgtcgt
     1021 tgtcggggtt cttgtccgag acgaggatcg aaccatcggc ctgcgcggtc agcgtcgccc
     1081 cgttcttcga tttcgcctcc gccttgtcga agacgatcca ctcgacgtcc ttgcgccact
     1141 tgccgtcgag ctgcgggctc tcgtcgagcc acttctgaaa ctcgcccggc atcgccgtct
     1201 cttcgtaggt ttgcagagcg gcggctttcg gctgatgctc tttctcccaa gcggcgaggg
     1261 cggcctttgt tttctcgggc gttacgtcca cgtcgaggtt gctgcggacc gtcgtcgtga
     1321 aggtggaaag aaagcgatag tagtcggctt gcgggatcgg gtcgaacttg tggtcgtggc
     1381 agcgggcgca gccgatcgag aggctcagca tcgcgctgcc ggtggtcgcg gccatgtcgt
     1441 cgagggcgtc gtaccgcgtc cgctcaactt cattggcggt gatctgcgtg ggaaacacgc
     1501 ccgcgccgag aaagccggtc gccttcagcg ccagcggatt cgccggctcg aattcgtcgc
     1561 cggcgagctg ccagcgcacg aaccggtcat agggcaagtc ctggttgaac gcctcgatga
     1621 cgaagtcgcg gaagtgatac gcaaaaggcc ggtcgtaatc ctgctcaaag ccgtggcttt
     1681 cagcaaagcg gacagcatcg agccagtgcc gggcccagcg ctcgccgaag tgagggcttt
     1741 caagcagcgt gtcgagcagc ttctcgtatg cccgcgggtc gatatctgcg acgaacttct
     1801 cgacttcctc gggcgtcggc ggcaggccca caagatcgaa gtacgcccgg cggatcagga
     1861 cgcgccgctc cgcgggcgaa ttcggcgtca ggcccttttc ctccagcttt gccagcacga
     1921 accggtcgag gtcgttcctg acccaggctt cgttcctcac agccggcggc tgcgggtggg
     1981 ccagtggctg gaaagaccag aactcgcggg ctttcgcggg tacgacggtc gccgtccacg
     2041 aatcggggtc gcgcgggttc tcgacgagcg gctggtcgta gggagcgccg aggtcgatcc
     2101 agcggctgat ggcggcgatt tgctcgtcgg gcagcttatc gcttccttcg ggcatgaacg
     2161 gcttctcacg gtgactgatg aagcggatga cgttgctgtc gatcgacttc cccgtgataa
     2221 ccgctggccc gtgcgagccg ccgcgcaaca ggcccttgcg cgtcgccaaa tcgaggcccg
     2281 attccacctc cgcccccccg tggcaatcga cgcaattatc gatcagcacc tgccgcacct
     2341 gcgatttgaa cagcctgagc ccttcctgga tcttctcgac gtgggccgga tcgaactcgg
     2401 gcttgggctc gtcggccgcc gcggacagcg acgcgagaaa accgaggatg acggccggga
     2461 cagcgctaga gcgggaggac atcacggcct cgcgggggag ggtcgagcgg cgaaaagcgg
     2521 cgccaagccg cgggcgggag cgacggcaat tgcggtgcga ttccgggaaa tcggccgtgc
     2581 catcgccata accgattcta acgtaagtcc cggcgggaca gcaacttcgc ggcttgtgcg
     2641 agcaaggttg ccccggaccc ttggcaacct atttttcagg cgtggggtgg attgtccctt
     2701 ttcttgctcc gctgaactgc atgccatgtg aggattgcca tgctctttcg ctctgcattg
     2761 ttggccatcg ttctgtccgt tgccggctgc aacatgcagc cgatgaaggt ctgcgtctat
     2821 atcgacaact ccggccacga gcccatgctc atcaccgtcg atggccagga ggaaatgacg
     2881 gtcccgtcgg gagaggttgc ggaattgaag ctgccgccgg gtgagcacca gtttctgatt
     2941 cgcgcgggga gcgagaccgt ctgcgatctg atgcggaacc tggagccgtc ccagcgcttc
     3001 ggcgtggcgc ggaagtatct cttcgatccg tataaaaacc accgctacca gacgtattac
     3061 gcccagtatg gcgaaagccg gctgggggac ttgatggaat cgacgctgtt cagcttccag
     3121 accgatccgg acgctcaatt acagtatgtg tacaagcaac tgctcaagga aatcgacctg
     3181 gtgccgagcg atgcctggaa cgacgtgacg ggtgtcgatt acgtgctgac cgccccgccc
     3241 gacgagatct atacgagcag catggaaaag cggcgggtgc tcgaccggat tgagcccgcc
     3301 ttctacgagc agtttttgga ggccaagcag gtgcagaatc ccacggatga ggacgtggaa
     3361 aagctgggcg acctccttga cgaagtgctg gcccagtcgc tgtaagcgat tgtggcgagg
     3421 cactttgcga ctgccccgcg gatttcgcgc ggccgctgat tgcattgctg tcatagcttt
     3481 gctagaacca cggggctttc tttggccgcg agcttcctcc gcggctgaac cggccccgca
     3541 gcaaggagtg caggcatgct cgttctcagc cgcaaggtgg gcgaacggat tctcatcggc
     3601 gatcagattg cgatcactgt cgtgcggatc aatgcgacga cggtgcgcct gggaatcgac
     3661 gcgcccagca agctggccgt cgtgcgggag gaactggccg aaggatcccc gccgtcttcc
     3721 gcgccatcgg gagatcccgc gaaactggct gcgcggtagg ccgccattgc tttgctaggc
     3781 tgaccgcaac gtgctcagca cgacgcttag atcgcctggc agcggggcga cgaattcgat
     3841 tggctctttt gtcgcggggt gggagagctt gatgcggcgg gcgtgcaagg cctggcggga
     3901 gagcacgacg tcggtggcgt tggcggcgag tgagcgagcc cctccggccg tgccatactg
     3961 ttgcacttcc ccgcgcgtga tctgcgaccg gccgccgtag agcttgtcgc acaagaccgg
     4021 acagccgata tgggcgaggt ggacgcgaat ctggtgcgtg cggccggtct tcggcagcac
     4081 gcggacgagg gcatagccgg gaaaacgctc gacaacttcg taaaacgtcg aggcgtcgcg
     4141 gctggtggaa tgaccggccc ggatggccat cttttcgcgc tggtgcgggt ggatgccgat
     4201 cggctgctga atgatgtcgc ggtcgcgatc ggggacgccg gcggtgatcg cgagatactc
     4261 tttctcggtg gtgcgggcct cgaactgggc ggccagaacg tagtgcatcg ggtcggtctt
     4321 ggcgacgacc atcacgccgc tcgtatcgcg atcgagccgg tggacaattc ccggccgcgt
     4381 cgggccgccg gctttgctga gctgctggaa atggaacgag agggcgctgg ccaacgtgcc
     4441 gctccagtgt ccgcgtgcag ggtggacgac catccccggc ggcttgttga ccgcaatgat
     4501 gtgttcgtct tcatacagaa tgtcgagcgg gatgttttcc gggtgcggcc cggccgtgtt
     4561 catcgagggc aggacgacgc ggacgcggtc gccggcgctg agcttgtagg cgaccttcgt
     4621 tccttgctcg ttgaccgtta cgccgccgga cccgatcacc ttgcggagct gcacgcggct
     4681 gtacttcggg agcgaccggg ccagaaacgc atccagtcgc aggccggcat cggccggagg
     4741 gacgatcaac tcaagcggct ccgccgagag gtcgattgcg gattgcagct tgcggcttgc
     4801 ggattgaccg ctgggaggct gggcggaagg agtaaccacg aatcacgcga ataacacgaa
     4861 tcggcagcga tgacgacagc acgctctcat tccggcttgt cctctttcgt cggattcgca
     4921 gtttcctctg ccggcttggc ttgggcgggc ttgtcttcct ccagcctggc ttctcctgtg
     4981 ttggcgtccg ccggtttcgg ctcttcggtt gttggcactt caggcttggg ttccggctgc
     5041 gtcgttcccg gctgtggcag cgtcggttcg ggcgaagcgg ggacgccggt gccaatatca
     5101 tcgaggccta aggttcctcc gggcaggctg atatcgggcc gatcgggcaa atcgttgggc
     5161 agccccggca agccgccgcc ggcgcccggc agcggagacg gtttggtagg cttctgctcg
     5221 gcgaaccagg cgagcaaggc cacggtacgc ggattttcca ttcgcttgac gtcgctggca
     5281 gccgccttgc cgattgccga atcggggcgc gaatctgcga cctgcttgta gtatttgagc
     5341 gcttcctcgg gctgacacag cgactcatac accttcccca gacccattcg cgctctggca
     5401 acgagatcgg gatcgctcgg cgccgcggcg gccttttcgt aatagcctcg cgctttttcc
     5461 aataagtctt tggcctgctc gcggtcgctg aacatcaggc cggcgccacg ggcgacattg
     5521 atgtcgccca gcgtctgtgc cgcccaaagt ccgacaaccg aactcggttt gtcgtcgagc
     5581 agattctgca aactcttctc cggatcgcgc tcgttcagcg ctgcgcggta gtcggcccac
     5641 tccttggccg tcgcggcgct ttctgtcccg aaataataga tgccgccgat caccgccagg
     5701 agcaccacga tcactgccac ggtgatatgc gtgccgtacg gcttcaggac gtcgatccgc
     5761 tcggtcagcc agtcggccag ttcgttgtgt tcgcgctcgt ggcgaagttg tcggtgggat
     5821 ttgacgacca tgttctcgtg caaatgagca agggagatag ggcccgcgcc attcagcctg
     5881 cgcgcaaata tatgcggcgc taagagatag cgtcaagggc ggtggcagga caggacgggt
     5941 tggaaaaccc gtcccacgag tcacttttcc gctgtctgat cgtcgcgggc aaaccacatc
     6001 atgtgctgcc aggggaggcc gtcgaattcc ttgacgagct tgaagccgtt ggccggccat
     6061 tccttcatga tctgggcctt gctcatcttg tgctcgggct tgatcggcac cttggggtct
     6121 tcggtgcgga attcgacgag gacgcaggcg cccgtgggcg acagggcctc ccgcatcgcg
     6181 gccagcatct gctcggggtg cgagaactcg tgatagacat cgaccagcag gatcaggtcg
     6241 atcttcccct tgggcagccg cggatcggtg aacgtgccca ggatgggcga gacattgcgg
     6301 atcccctgct cgtcggcccg ctcgttgagg aatttgagca tctcgggctg gatatcgaca
     6361 ccgtagacgt ggccgtcatc gccggtcatt ttggccattt gcagcgcgta gaaaccgttg
     6421 ccacagccca tgtcgcagat cgccatgccg cgcttgaggc ccaggttggc gagcatcagc
     6481 gagcagcgct cctcctgctc gcggttgtcg cgcgtcagcc actccgcccc gaggtagtgc
     6541 atcgtctggg cgatttgccg gcctttgtaa gccttcagcg ccggtggcgt gcgcacggac
     6601 tttggtgcag cgggattttc cggcgcttct tgcgcggcca gccgtccgcc catagcaata
     6661 gccacgatga cgaagcagac aagcaagcgg cgcaggggcc agatcgggag gagaagattg
     6721 cgatgcatgc cgcgaagacc ctaaagtgtg caaaagccgg gaccggcaaa cgtgccgcta
     6781 ccgtcatcct aagctgtggc cgaacgggcc ggcaaccttg tgagatatcc ggagcgaatt
     6841 gtgcgatttc agatgcagca gaagtggctg agctggggcg attcgttcgt catccaggac
     6901 gaagaggggc gggaagtact gtacgtgcaa ggcgcggtct tttcgtgggg caatcagctc
     6961 tcgattcgcg agcccggcgg cctggaagtt gccgaagtca agcagatcgt gtttagctgg
     7021 gggcccacct acgaaatcta ccgcaacgcc gcgctcaccg cccgcgtgac taaggagctg
     7081 ttcaccttct tccattgccg cttcgcgatc gacgtgcccg ggccggacga tctggaggcc
     7141 gaaggggact tctggcagca cgaatacgcc attcgccgcg gcgcgcagat cgtggccacg
     7201 gtttccaagc aatggttcgc ttggaccgat acctatggcg tcgaagtcgc gcccggcgaa
     7261 gacgcgcacc tggtgctggc ttgcgccgtg gtcatcgact gctgctgcca cgacaaaagg
     7321 aatgactgag cggcacggcc tcatgcgatg gagttcggcg ttacctgctc tgctcgcgcg
     7381 ccgcggcatc cgcggaattc agacgcgttc gcatttcatc cagtgacgtg atgcgctcaa
     7441 caatctccgt gaagtaattg tgctggcgct gatcggtgcg gtagttcggg atatgccaca
     7501 cctcggtccc cttgtgctcc atccacatct ccagatacgt catcgctgcc ggaacgtagg
     7561 tccattccag gccgctgtcc gatttgcgac attcgatgga gaggagcatt tcggcatcgg
     7621 tcccatcttg ccccgtaaat acgaacaccg cgccgtcgat aatcttgtgc tgacccttct
     7681 ccagttcggc cgaatcgagt cgataaatcg gcgtcggtaa gagccgcagg cgcgccttgc
     7741 cggaatcatc gggtttgacg gtcgtgccgc tgaattcatg ggcgagcgag cgcatctgcg
     7801 tgagccggcg gcttcgtgcg agcggcgtag actctgcgga ggcgggagcc ggcgctccgg
     7861 gaatcaactt cggcgtttct ccttcaggac gccagacaag atctggcagt gaattgatct
     7921 tggtcggctg cggggcgcgc tgcgtgagca cgtgaaactc gtggaaaaag tcccacctcc
     7981 cttggacggg aaatgagccg atcccgcccg cgataaccgc ccgcccctcg tacgtccaga
     8041 tgtagacctc gcccgagttg gctgaaaccg tgttggcgac cgactggtag tcgtgccccg
     8101 tataggtcat tgcaaccggt tccaggacca gcttttgttt gccctcggca tcgagcgtga
     8161 attcgtattg ctgggcctta tggcggtaat acgacttgag cagctggagc gtttcagcgc
     8221 cttcctcggc cccagccgct tcctggccgg cacagcgcag gctggcgacc agcaccatca
     8281 tcgtaaatgc cagcgccgtt cgaagtccca tcgccatggc cagcgccccg tgggcaagga
     8341 agcagtccac tgccaatgaa tgctagagac gcgtggcggg aaatgcaacg catggtgttg
     8401 cgtagcgggt caatagagtc gcctttcgct ccgcgaaagc gccttcgttc gcagaacgaa
     8461 cgacgacatc ttcggtcgtt cgttgttacg cggacgtgac gcgccggggc gggcgatgcg
     8521 cctacgattc gaggtgaagg cgacaggaca tgatcttgtt ctcgaaactc cctgcggcca
     8581 actggtggac gtgcctcacg gtcgtcgcgt gtgccgccat cctgctcggc aacgcgatct
     8641 atttcgacgg cggaccgccg ccgcgattcc tgttggaaaa gggggagtgg gcccgcaatc
     8701 cctggtggtt ggccgcgttc tatttccaca tcgtcggggc ctcggtcagt ctggctgccg
     8761 ggacgccgct gatgtttcca cctggacgcg aaaacatcct gcgtggcatc gccgcctggg
     8821 gatcgtctat gtgaacgctg tcctgtggat ggccgccccc gccgggctgg cgctggcgct
     8881 ggtcgccaag ggcgggctgt ggggcacggc gggatttgcc ctggccgggg ccttctggtg
     8941 gcagaccacc tggtccggat acctggccat ccgccgcggc gacgttgcgg gccatatccg
     9001 ggcaatggtc cgctcctatt gctgggccct ctcggcgccc gccttccgtg tgattcaggc
     9061 cgccctgttc ctggccgggt tcgcggacgg catcaactac gttctctccc tttggttgag
     9121 cattgccgcg agcgtgtggc ttgcggagtc gttccttttt cgtgaccgac gaaagcaact
     9181 ggaagtcctt ccgtcttcgc ccagtgcagg agttctcaca tgaaccgatc gctcattgtt
     9241 cccgcgtgtt gcagcctgtt ggcgttcacg gcgctattgg cggcgctgca tggagttctg
     9301 gcccgagaaa ccggtcagcc gcaaactgcc ggggggcagg ccaatcgtga acggaatccc
     9361 acggagctgg cgtattcgtc cgtcgccgtt ccccgactcc agggcgaacg gctg
//
LOCUS       DASUGB010000307         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1701946, whole genome shotgun sequence.
ACCESSION   DASUGB010000307 DASUGB010000000
VERSION     DASUGB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701946"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..907
                     /locus_tag="VFV87_05915"
     CDS             554..907
                     /locus_tag="VFV87_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEX5103324.1"
                     /translation="MTEPKLEVPAELRDLAEKTIDQAEKAFGMFFDAAGKSMTSMPGA
                     GTEISRQALSFTEQNMKAAFEHARKLVHATDLQEAMRIQSEFLRSQFTNAGEHMRQIT
                     GGVISAAKDSTKGKF"
     gene            complement(917..1177)
                     /locus_tag="VFV87_05920"
     CDS             complement(917..1177)
                     /locus_tag="VFV87_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103325.1"
                     /translation="MNIEPSETPQPGMAERAIDTATDVSRTLTEVSAALQTAVGRLSD
                     AITAARQPGMPLATVSAITREAPLASLFVAFLFGVAVARRRR"
     gene            1344..1628
                     /locus_tag="VFV87_05925"
     CDS             1344..1628
                     /locus_tag="VFV87_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103326.1"
                     /translation="MAKHLVEDRLNRHGGAPLKFAIAVAVIGVLGMLIVDHGPWNKPK
                     HQPAHIASYSTTGEAARAVGAQVMPTEPKLQVEPEPPVPKPLYPANPTPR"
     gene            complement(1666..1851)
                     /locus_tag="VFV87_05930"
     CDS             complement(1666..1851)
                     /locus_tag="VFV87_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103327.1"
                     /translation="MFYVAAMLTILSGLFYSASHHEIGSLGVAMCQYGSPFCDKPFLV
                     LVAAGLAACWGAFVSVR"
BASE COUNT          480 a          694 c          681 g          417 t
ORIGIN      
        1 ccttgacggg cctcggcctg gtcgacgttc gcgacgaagg gatcgtgctg acgccggccg
       61 gaatcgccgc gctgaacggg acgtagtcag cgcgccttca ggaagctctc accggagtcg
      121 caaggcgtgc agcgataggt caggacgtcg tagttctggt cgcgcggctc gatcgtcgcc
      181 agccgcatct gttcgccgca tttcatgcaa ggcatttcta tgctggtttg aaaacgtgtg
      241 caggttggtg tcggccgaag tgcttgcagc atcgtatccc catgagactt aaatccagag
      301 acgcaacgcc acccacagtc gggctctaac cccgaatcac tatgccacac tgttgccacc
      361 attggaatcg cgcgtcgacc cccgtgcggc tacggtcggc gacaaaagga aatcagtcct
      421 taggcaattc gttgcgaccg atcggctgaa tgccggcggc gcgcgtcttt gccgggcgaa
      481 gcctggtcca atacggcaca gacgccgccg ggaattgtgc taacctgcca aggtcgacat
      541 tggagaagcc accatgactg aaccgaaact cgaagttccg gccgagctgc gcgatctggc
      601 cgagaaaacc atcgatcagg cggaaaaggc gttcggcatg ttcttcgatg ccgccggcaa
      661 gtccatgacg tcgatgcccg gggcgggcac ggaaatttcc aggcaggcgc tatcgtttac
      721 cgagcagaac atgaaggccg cgttcgagca tgcccgcaag ctggtccacg cgaccgacct
      781 ccaggaagcg atgcggatcc agtccgaatt cctgcgcagc cagttcacca atgccggtga
      841 acatatgcgc cagatcaccg gcggcgtcat ctctgccgca aaggattcga ccaagggcaa
      901 gttctgagga gcggcgctag cgccgccggc gcgcgaccgc gacaccgaac agaaaggcga
      961 cgaacaggct ggccagcggc gcttcgcggg ttatcgcgct gacagtcgca agcggcattc
     1021 caggctgccg ggccgccgtg atcgcatcgc tgagacgccc gaccgccgtc tgcaacgcgg
     1081 cggatacctc tgtgagcgta cgcgacacat ccgtcgccgt gtcgatggcg cgttcggcca
     1141 tgccgggctg cggtgtctct gagggttcga tattcacgtt ctgctgctca cgggattacc
     1201 cggcattggc cgggccacgg ctgatgccat gacaattcgt ggccatgcca tttgttcctg
     1261 gatgtccttg cagtggggca ataaggaacc ttcgtcgtcg cgggggttta atttcatcag
     1321 aggaaacgac cacggatttt cagatggcga agcatttggt ggaagatcgt ttgaatcgac
     1381 acggaggcgc accgctcaag ttcgcgattg cggtcgcggt tatcggcgtg cttggcatgc
     1441 tgatcgtcga ccacggacca tggaacaagc cgaagcacca gccggcccat atcgcgagct
     1501 acagcacgac cggcgaagcc gcacgggcag tgggcgccca ggtcatgccg accgaaccga
     1561 aactgcaagt ggagcctgag ccgcctgttc cgaagcccct gtatccggct aacccgacgc
     1621 cgcgataaag ctcgacataa attggcgggc tgacgaatga gcgcgtcagc gcacgctgac
     1681 gaatgcgccc cagcacgcag ccaaccccgc ggctaccagc acgaggaatg gcttgtcgca
     1741 gaacgggctg ccatactggc acatcgcgac acccagcgag ccgatctcgt ggtggcttgc
     1801 cgaatagaac aggcccgaca gaatcgtgag catggcagca acgtaaaaca tctgaccgcc
     1861 tccttgcgct ctgttgggcc gcaggttgcg ccatccacgg cccaacgatt tcgtctcgat
     1921 gtcgttgtgc cggcttgaat ctggtttgct ttggccactt gacgcgtcgg gcaaatcagg
     1981 ggcacattgg catcatcgca gatatcgtac agcccgcacc ggaaacggcg gcgggctttt
     2041 cagttcacac tacgatcggc caaagccgtt gcaatttgtc gaaaccgttt cgtcgaattg
     2101 cctgcaatcg ttgacgattc aaaaacggat tgacatgtcg ggcaaatcac cggcataacg
     2161 ccaccatcac agaaattgca aagcccgcac ggagaaatcc gccgcgggtt ttttgttttg
     2221 gaatttccga atcggacggc ggccgcacat cgacgccgca actcccaaca cc
//
LOCUS       DASUGB010000308         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_312700, whole genome shotgun sequence.
ACCESSION   DASUGB010000308 DASUGB010000000
VERSION     DASUGB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312700"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VFV87_05935"
     CDS             complement(<1..1620)
                     /locus_tag="VFV87_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103328.1"
                     /translation="MAEHRWHWLPISLLTGGVALVCAAAAANFVALGWWELPILTGCA
                     AALAAAWWFRRKLSAAELELEAFRKRLADDENRLNTQRSEFEQLRLTIQSELSQEASR
                     LDRREQNLNQRLVTYCEWMEFPQPLELANQAAVAAAELSDLAKKDRQMLELLQAETKL
                     LYDNILQNKYASGDRVLLPVVRDDLVVLITKVARIYQPHLKQESVLLDASLARIFRAT
                     SRASLQMLVVLDELPLNVKDASLITLYGYIRNAVSTWRTYKSTEPYWPYVNAAWYVSR
                     FALGANPISLGAWWFVSTLGTRGAQALAQHVINRQALAVLSNVVRVIGFEVAGIYGGD
                     FRHRDTNWIYAAELTELLSQFPLSRDSLAHALREVGALQLRSEYDRTFLYRCLAGRKS
                     AGPAQYHAADVLTMDERRAVATRLERFLETFIHGKSAEKISRWKAAAEQRLDLKISVA
                     LTISTGAVHEQIVDAVRSLASFLVATKQLEPPEAVKRLENTLLAGELTAEDRQRLLAD
                     LAENASFFFEHPDLDPDGDLVEKYLDDLAALH"
     gene            complement(1724..3208)
                     /locus_tag="VFV87_05940"
     CDS             complement(1724..3208)
                     /locus_tag="VFV87_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103329.1"
                     /translation="MSTSVKTGCAWLLLIASSTAANFHVAPDGTARGDGSAAQPWDLA
                     TALAAEDVVRPGDTVWLHAGMYRGGFTSNLEGLPDNPIVVRGAHGERATIDTQPRDER
                     DNGLLLLGGADVVFRDFEVTCSHPQRETKIGGSWPEDIRRGSVDIRGDRISAVNLVVH
                     DCASGFGFWAEGEGGEISGCLIYYNGWKGPDRGHGHGIYTQNARGTKRIADNIVFHQF
                     AYGIHAYGSEKASLTGFDVEGNICFENGCLTQPGDNAPGIMIGGATPAERIAVRDNVI
                     VGGRIRLGYPWGVANEDVVCTGNYADQGLVVRDFHRATISKNTLVASSSVAGLEGAAD
                     LLLSGLKWNDNEYYVTDGRWGECSVVENGKSRGLSFAEWQKHTGFDANSTFTKGQPSK
                     LRVFVRPNVHEPGRANIAIINPVGLAEVAVDLSGVLKPGEAFRIVSAKDFFGRPVLAG
                     TYDGGAVRVPMKPVAPPAPVGMPAAELPMTEPHFAAFVVLPGKA"
     gene            complement(3225..>3421)
                     /locus_tag="VFV87_05945"
     CDS             complement(3225..>3421)
                     /locus_tag="VFV87_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103330.1"
                     /translation="DAALDHGGRYYLPYRLHATPEQFARAYPQGREFFELKSKYDPEK
                     LFQSEFYLEYGKQEPRDLPR"
BASE COUNT          606 a         1122 c         1069 g          624 t
ORIGIN      
        1 gtgcagcgcg gcgagatcat cgaggtattt ttcaaccagg tcgccatccg gatcgagatc
       61 gggatgctcg aagaagaacg aggcgttctc tgccaggtcg gccaacagcc gctgccgatc
      121 ctcagccgtc agctcgccgg cgagcagcgt attttccagg cgtttcaccg cttccggcgg
      181 ctcaagttgt ttggtcgcga cgagaaagct cgccagcgag cggacggcat cgacaatctg
      241 ctcgtgcacc gcgccggtcg agatcgtcag ggcgaccgaa atcttcagat ccagacgctg
      301 ctcggcggcg gccttccagc ggctgatctt ttcggccgac ttgccgtgga tgaacgtttc
      361 caggaaccgc tccagccgcg tcgccacggc ccgccgctcg tccatcgtga gcacgtccgc
      421 cgcgtgatac tgcgccggcc cggcgctctt gcggcctgcc aggcagcgat acaggaacgt
      481 gcggtcgtat tcgctgcgaa gctgcagcgc gccgacttcg cggagggcgt gggccaggct
      541 gtcgcgcgag agcggaaact ggctcagcag ctcggtcagc tcggcggcgt aaatccagtt
      601 cgtgtcgcgg tgccggaaat caccgccata aatgcccgcc acctcgaagc cgatcacgcg
      661 gacaacgttg ctcagcaccg ccagcgcctg tcggttgatc acgtgctggg ccagcgcctg
      721 cgcgccgcgc gtgccaagcg tgctgacaaa ccaccacgcc ccgagcgaaa tgggattggc
      781 cccgagcgcg aagcggctga cataccaggc ggcgttgacg tacggccagt agggctcggt
      841 cgatttgtag gtccgccagg tgctgacggc gttgcggatg tatccataca gcgtgatcag
      901 gctcgcgtct ttcacgttga gcggcaattc gtccagcacc acgagcattt gcaggctggc
      961 ccggctggtg gcgcggaaga tgcgcgccag actggcatcc agcagcacgc tttcctgctt
     1021 caagtgcggc tgatagatgc gggcgacttt cgtgatgagc acgaccaagt cgtcgcgaac
     1081 gaccggcaac agcactcggt cgccgctggc gtatttgttc tgcaggatgt tgtcatacag
     1141 cagcttcgtt tcagcctgca gcaactccag catctgccgg tctttcttgg ccagatcgga
     1201 caattcggcg gcggccacag cggcttgatt ggccaactcc agcggctgcg gaaactccat
     1261 ccactcgcaa tacgtgacca gccgctggtt caggttctgc tcgcgccggt cgaggcggct
     1321 ggcttcctgg gacaactcgc tttgaatcgt caggcggagt tgctcaaact ccgagcgctg
     1381 cgtattcaag cggttttcgt catccgcgag gcgcttgcgg aatgcttcca gctccaattc
     1441 ggcggcgctg agcttgcggc gaaaccacca ggccgccgcc agagctgctg cgcagccggt
     1501 caggatcggc agctcccacc agccgagcgc cacaaagttg gccgccgccg cggcgcagac
     1561 cagagccacg ccgcccgtca agagcgaaat cggcagccaa tgccagcggt gttcagccat
     1621 cgagccgtcg caaaagacag ggagcgtcag ctattgtact tcgctccccc cggggcgttt
     1681 ctcggcggcg gaggttcgag ggcgccgacg caggatgatt gcctcacgct ttgcccggca
     1741 gcacgacgaa tgccgcgaag tgcggttcgg tcatgggcag ctcggccgct ggcatgccaa
     1801 ccggcgcagg cggcgcaacc ggtttcattg ggacccgcac ggcgccgccg tcataagtgc
     1861 cggccaaaac cggccggccg aagaaatcct tggcgctgac gatgcggaac gcctcgcccg
     1921 gcttgagcac accggacagg tccaccgcga cctcggccag gcccactggg ttgatgatgg
     1981 cgatattcgc tcgcccgggc tcatgcacgt tcggccgcac aaacacccgc agcttgctgg
     2041 gttggccctt cgtaaaggtg ctgttggcgt cgaaacccgt gtgcttctgc cattcggcaa
     2101 acgacaagcc gcggctcttg ccgttctcaa caaccgaaca ttcgccccag cggccgtcgg
     2161 tgacgtagta ctcgttgtcg ttccatttca gtccgctcag cagcaagtcg gcggcgcctt
     2221 ccagtccggc gacgcttgac gaagccacca gcgtgttttt gctgatcgtg gcgcggtgga
     2281 aatcgcggac gacgaggccc tgatcggcgt aattgcccgt gcagacgacg tcttcgttcg
     2341 ccactcccca cggatagccg aggcgaatcc tgccgccaac aatcacattg tcgcgaaccg
     2401 cgatccgctc ggccggcgtg gcgccgccga tcataatgcc cggagcgttg tcgcctggct
     2461 gcgtcaggca gccgttctcg aagcagatgt tgccttcgac gtcgaagcct gtgagcgacg
     2521 ccttctccga gccgtaggcg tggatgccgt aggcgaactg atggaagacg atgttgtcgg
     2581 cgattcgctt cgtgccgcgg gcgttctgag tgtaaatacc atggccgtgg ccgcggtccg
     2641 gccctttcca gccgttgtag tagatcaggc agccggagat ttcgccccct tcgccttcgg
     2701 cccagaagcc gaacccgctg gcgcagtcgt ggacgacgag attgactgcc gagatgcggt
     2761 cgccgcggat atcgacgctg ccgcggcgga tatcctcggg ccacgagccg ccgatcttgg
     2821 tttcccgctg cggatggctg caggtcactt caaaatcgcg aaacacaacg tccgcaccgc
     2881 ccagcagcag caggccatta tcgcgctcgt cccgcggctg ggtatcgatc gtcgcccgct
     2941 cgccgtgcgc gccacggacg acgatcggat tgtcgggcag gccctccagg ttcgacgtaa
     3001 atccgccccg gtacattccc gcgtgcagcc ataccgtatc gcccggccgc accacatcct
     3061 ccgccgccaa agccgtcgcc aagtcccagg gctgggcggc ggatccgtcg ccccgcgccg
     3121 tgccatcagg agcgacatga aagttggccg ctgtggacga cgcaatgaga agcaaccacg
     3181 cgcagcccgt tttcacgctc gtggacattc gaggcactcg cagtttatcg cggaaggtcg
     3241 cgcggctctt gcttaccata ttccaagtaa aactcactct gaaacagctt ctccggatca
     3301 tacttgcttt tgagttcaaa gaactctcgg ccctgcggat aggcccgggc gaattgctcg
     3361 ggcgtcgcgt ggagccggta gggcagatag taacgaccgc cgtgatcgag cgcggcgtcg
     3421 a
//
LOCUS       DASUGB010000309         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_34774,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000309 DASUGB010000000
VERSION     DASUGB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34774"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VFV87_05950"
     CDS             complement(<1..1959)
                     /locus_tag="VFV87_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5103331.1"
                     /translation="MLTSLINLSLNNRFLVIALFTLMAVGGAVSALYIPIDAVPDMTN
                     TQVLVLTKAPGLSPLEVEQYVTNRVENAMGGLPRVSEMRSVSRFGISSITLVFEEGTD
                     IYRARQLVNERITVVQGELAPGHGPPEMGVLSTALGEVLQFEVRSVGPGGRPVRDAME
                     LRSILEWQIAPQLRQVKGVTEINSIGGFYKTFEVRPDPNRLAAERVGLDELLSALARN
                     NANAGGGYVVHEGEQRFIRGEALLRDIKDIENVVVRSGEADLPILIRDVAEVAIAPLT
                     RQGSATRDGRGEVVTGMVIMLLGENSRTVVHRAKERLKEIQATLPADVKLEILYDRAD
                     LIQRTLHTVVNNLLEGGILVILVLLVLLGNWRAGVIVALAIPLSMLFATNIMLATGIT
                     ASLMSLGAIDFGLIVDSSVIMVENCIRRIAHHSEGKTHLEVIREAAIEVRKPTMFGEL
                     IIAVVYLPILTLQGTEGKLFKPMALTVLFALAGSMVLSMTLMPVLAYTSLPKSLEEKD
                     VWLIRLIKWIYRPLVERALAFPLLTAGLALALLAISVPIGMNLGAEFMPRLDEGDILI
                     EAVRLPSAALEGATAISTDIERALQEFPEVKTVFCKTGRPEIANDLMGTHQTDVWVIL
                     HPERDWPEHKTRDELIEEMSAVLNRQVLG"
     gene            complement(1972..3474)
                     /locus_tag="VFV87_05955"
     CDS             complement(1972..3474)
                     /locus_tag="VFV87_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5103332.1"
                     /translation="MHMRSWFSWSAAPQTGRWLRALLGAVAIGAALLLLLALRMEWIS
                     ILPGESAVDSAGDEAAPAHGAVTSLQLPPEKLAAANLHIAAAEVRDVQPTRTVPATIT
                     YDAALRLPVNAPVASVVLKVLVEPGQDVTANQPLAVLSSPEVGLARDEVLRRQADLAL
                     ARKQQTFSDETATNVVRLLDLLKQRPRPEELEKSLEGRALGDYREKIIAPYSKLVLAD
                     LRLKGTSDLQESGALSRRTLEERNSDREVAVAQFTAACESAKFSASQEREKAAAAAEQ
                     AERLVAVAEQTLANLLGPMTDMTAVTERNKLSELMLLSPIAGRIEERQAVEAARVAAG
                     EPLYLIADTSVMWISAEIHERDWQALDAARQATITARVPALGDGSLTAQVRFVGAEVA
                     ADTRSVPLVAEVANPQGKLRPGMFAWALVPLGPPRQSLAVPPGAIMRHDNQAFVFVPE
                     GQNGFRRVDVQTGIESATGIEILAGLKSGERVVDRGAFFLKSELLLEREE"
     gene            complement(3578..3892)
                     /locus_tag="VFV87_05960"
     CDS             complement(3578..3892)
                     /locus_tag="VFV87_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103333.1"
                     /translation="MEPIVELGNGSGPAWSASNGETAPHRGCENESGCICRGATLAHG
                     VDLAALQPVQMNLMLVESYTAQAGLVEADSAAAKGIFDDPCRLPPLSGRILRARHSSL
                     VI"
     gene            complement(4545..>5844)
                     /locus_tag="VFV87_05965"
     CDS             complement(4545..>5844)
                     /locus_tag="VFV87_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5103334.1"
                     /translation="GVRTMIGVKVFGDDLNKIQAVSEQIAAVLRDVPGAADVFPDQIV
                     GEGYLEIDIDRDKAARYGVNVGDVQDVVEVALGGKMITMTVEGRERFPVRIRYARAFR
                     EDEQSVKNLLISAGGAMAMAAGEGGTRGMNGAGNGGSNGAAMLTSAARSESPSALNPF
                     PNGVAQIPLAQIADVRIVEGPSMIKSENGLLRSYVQLNVRDRDIVGFVEEAQRAVADK
                     VTLPQGMYIEWSGQFEHQVRAKRTLTVIVPIVVLIIFVLLYVTYHDLADATLMMLAVP
                     GAVAGGVLFQYLFGFNFSVAVWVGFIACFGLATETGVVMLVYLREAVANRGGLENIQS
                     LEELRQAILEGAVHRLRPKLLTEGTTIIGLAPMLWATGTGAEIMQPMAAPVLGGILVA
                     DEVIDLLLPVLFYHVRVRRWKRMHSSRVMEGSQPQTYARR"
BASE COUNT         1108 a         1886 c         1788 g         1062 t
ORIGIN      
        1 gcccagaacc tgccgattga gcaccgcgga catctcttcg atcaactcat cgcgggtttt
       61 atgctcgggc cagtcgcgtt cgggatgcag gatgacccag acgtccgtct ggtgcgtgcc
      121 catcagatcg ttggcaatct cgggccggcc cgtcttgcag aacacggtct tcacttcggg
      181 gaactcttgg agcgcccgct cgatatcggt tgagatcgcc gtcgcccctt caagcgcggc
      241 actgggtagc cgcactgcct caatcaggat atccccctca tccaggcgcg gcatgaattc
      301 cgcgcccagg ttcatcccga tcggtacgct gatagccagc agggccagag ctagtcccgc
      361 cgtgagcagc ggaaacgcca gcgcccgttc gaccagtggc cggtaaatcc acttgatcag
      421 gcgaatcagc cagacgtcct tctcctcgag agatttgggt agcgaggtat aggccagcac
      481 gggcatcagc gtcatcgaca gcaccattga gccggcgagg gcaaacagca cggtcagggc
      541 catcggcttg aaaagtttgc cctcggtgcc ttgcagcgtg aggatcggca gatagacgac
      601 ggcgatgatc aactcgccaa acatcgtcgg cttgcggact tcaatcgccg cttcgcgaat
      661 cacttccagg tgcgttttgc cttccgaatg atgggcgatc cgacggatgc agttttcgac
      721 cataatcacc gaagaatcga cgatcaggcc gaagtcgatc gctcccagac tcatcaggct
      781 ggccgtgatg ccggtcgcca gcatgatgtt tgtggcaaag agcatcgaca gggggatggc
      841 gagggctacg atgacgcccg cccgccagtt ccccaggagc acgagcagca cgaggattac
      901 caggatgccg ccttccagca ggttgttcac gaccgtgtgg agtgtccgct ggataagatc
      961 ggcccggtca tagaggattt ccagttttac gtcggcgggc agtgtggctt ggatttcctt
     1021 gagccgctct ttggcccgat gcaccacggt gcggctgttt tcgccgagca gcatgatgac
     1081 catgccggtg acaacctcgc cgcggccatc tcgggtggcg gagccctggc gagtgagcgg
     1141 ggcaatggcg acttcggcca cgtcgcggat tagtatcggc aggtccgctt cgccactgcg
     1201 gacgaccacg ttctcaatgt ccttgatatc ccgcagcaag gcctcgccgc ggatgaaccg
     1261 ctgttcgcct tcatgcacga cgtaaccgcc ccccgcgttg gcattgtttc gcgccagggc
     1321 gctgagcagt tcatccagcc cgacgcgttc ggcggccagg cggttggggt cgggtcgcac
     1381 ttcgaatgtc ttatagaagc cgccgatcga gttgatttcg gtgaccccct tcacttgtcg
     1441 caattggggc gcgatctgcc attccagaat cgagcgcagc tccatggcgt cgcgcacggg
     1501 ccggccgccc gggccgacgc tgcggacctc aaattgcagc acctccccca gggccgtcga
     1561 gagcacaccc atctccggcg ggccgtggcc cggcgccaat tcaccctgga ccaccgtgat
     1621 ccgctcgttc acgagctgac gggcgcggta aatgtcggtt ccttcttcga aaaccagcgt
     1681 gatggacgaa atgccaaacc gcgaaacgct ccgcatctcg gacacccgcg gcaagccgcc
     1741 catcgcgttt tccacgcgat tggtgacgta ctgttcgacc tccagcggtg agagacctgg
     1801 cgccttggtc agcaccagaa cctgtgtatt agtcatgtcc ggcacggcgt cgatcggaat
     1861 gtaaagcgcg ctgacggctc ccccgaccgc catcagcgtg aacagtgcga taaccaggaa
     1921 gcggttattg agcgacaggt tgatgaggga agtgagcatg gtgtttctgg attactcctc
     1981 ccgttcgagc aggagctcgg atttcagaaa gaacgcgcca cgatcaacga cccgctcgcc
     2041 cgatttgaga ccggcgagga tttcgattcc cgttgccgat tcgattccgg tttggacatc
     2101 gacccggcgg aagccgttct gaccctcggg cacaaagacg aatgcctggt tgtcgtgtcg
     2161 catgatcgcg cccggcggca cggcaagcga ttgccgtggc gggccgagcg gcaccagggc
     2221 ccaggcaaac atgccggggc ggagcttgcc ttggggattg gccacttccg cgaccagcgg
     2281 caccgacctg gtgtcggcgg ctacctcggc cccgacaaat cgcacctgag cggtcagcga
     2341 accgtcgccc agggccggca cgcgcgcggt aattgtggcc tggcgggctg cgtcgagggc
     2401 ctgccagtcg cgttcgtgga tttccgcaga aatccacatc acgcttgtgt cggcgattag
     2461 gtagagcggc tcgcccgcgg ccacgcgggc cgcctcaacc gcctggcgtt cctcgattcg
     2521 gcccgcgatc ggcgagagca gcatcaactc gctcaacttg ttccgctcgg tcacggccgt
     2581 catatcggtc atcggcccca gaagattcgc caaggtctgc tcggccactg ccaccagccg
     2641 ctcggcttgt tcggcggcag cggcggcttt ttcgcgctcc tggctggcgg agaatttggc
     2701 ggattcgcag gccgcggtaa actgggccac cgctacttcg cggtcgctgt tgcgctcttc
     2761 cagcgtgcgg cgacttagcg cgccggactc ctgcaaatcg ctcgtgccct tcagccgcag
     2821 gtcggccagc accagcttgg aatagggcgc aatgatcttc tcgcgatagt cgccgagtgc
     2881 gcggccttcg agcgattttt ccagttcctc cggtctcggc cgctgcttga gtagatcaag
     2941 caagcggacc acgttggtag ccgtctcgtc gctgaaagtt tgctgcttgc gggccagggc
     3001 cagatcggcc tgccgccgca gaacctcgtc gcgcgccagg ccgacttcgg ggctggaaag
     3061 caccgccagt ggctggttcg cggtgacatc ctgtccgggt tcgaccaaaa ccttgagcac
     3121 gacgctcgcg accggcgcat tgaccggcag gcggagcgcg gcgtcgtagg tgatcgtggc
     3181 ggggaccgtg cgcgtgggct gaacatcgcg aacttccgcc gcggctatgt gcaaattggc
     3241 cgccgcgagc ttttccggcg ggagctgcaa ggatgtcacc gccccatggg cgggcgccgc
     3301 ctcgtcgccg gctgagtcca cggcgctttc acccggcaag atactgatcc attccatccg
     3361 taaggcgagc agcagcagaa gcgcggcccc gatcgccacc gcgcccaaca aggcccgcag
     3421 ccaacgaccg gtttgcggcg cggcgctcca cgagaaccaa ctgcgcatgt gcataaggaa
     3481 actcccacga acgacggacc agaatcaaaa gaaggctcgc accaggcagc accgcaggcg
     3541 tgcgtccagc ggcaagcgac ggcagttagc cgtgaggcta gatcactagc gaagagtggc
     3601 gcgcgcgcag tatccgccca gaaagtggcg gcaatcgaca gggatcgtcg aaaatgccct
     3661 tcgctgcagc cgagtcagcc tcaaccagcc ccgcctgggc ggtgtagctt tctacgagca
     3721 tcaggttcat ctgcaccggc tgcaaagcgg ctaaatcgac accatgagcc agcgtcgccc
     3781 cacgacaaat gcaacccgat tcgttctcgc aacctcggtg cggcgcggtt tcaccgttcg
     3841 atgccgacca agcaggaccg cttccgttac ccaactccac aatcggttcc atcgcacagt
     3901 gcaggagcgc aacgactacc aagagcgcgg cagcgatcgt ggtacagcgg cgatggtagg
     3961 atcccgtcaa catcaagcgg caatcaacat gaattggggc tcagtcgata ccaaaagtct
     4021 acctggccga ctaattaatc gcaaatcgcc acattcggcc gcccgccacc gccatttttc
     4081 aaccgccctc aacgaacaat ggcagacaaa cgaccgaaaa gccaatgccg gggcgctagc
     4141 cgagagggtt ttcgcgtagc aatcagaaca tacagacccg agtaacggta ggaccggggt
     4201 ttggccgatc gttcgagaga cggtaccgct cgaaactgca ataccaacgc gtcgtggatg
     4261 attggtgaca gcacatcaca atggcacgca cagtggattg gcctcagttg cttgcaggtg
     4321 tctagcgcgt gtgatggagc ctgcgtcgga catcgctcca cgcgaattca cgcagttcca
     4381 aactgcaggc ggttggacca acaagcgcaa gttgcggccg gcgcgaactc gcagcggtgt
     4441 cccgcgggaa gagacaccgc tgcagctggc aagagcttcg ttgcgcgcgt ggccgatgca
     4501 accgctgcag gtttgctccg cggcgaatct gcaggcggtc ttcttcacct ccgcgcgtac
     4561 gtttgcggct gagagccctc catcactcgg gaagagtgca ttctcttcca ccgccgcacg
     4621 cgcacgtgat agaaaagcac cggcaggagc aggtcgatca cttcgtcggc gacgaggatg
     4681 ccaccgagga cgggggcggc catcggctgc atgatttccg cgccggtgcc ggtggcccag
     4741 agcatcggcg ccaggccgat gatcgtggtc ccttcggtga gcaatttcgg ccgcagccga
     4801 tggacggcgc cttcgagaat cgcctgccgc agctcctcca gtgactggat gttttccaaa
     4861 ccgccgcggt tggcgaccgc ttcgcgcaaa tatacgagca tgacgacgcc cgtttcagtc
     4921 gccagcccaa agcacgcgat aaacccgacc cacaccgcca cgctgaaatt gaagccgaac
     4981 aggtattgaa agagcacgcc tccggccaca gcgcccggca cggccagcat catgagcgtg
     5041 gcgtcggcga gatcgtggta ggtgacgtac aagaggacga aaatgatcag cacgacgatg
     5101 gggacgatca ccgtgagcgt ccgcttggca cggacctggt gctcgaattg gccgctccac
     5161 tcgatgtaca tgccctgcgg caacgtgacc ttgtcggcca ctgcccgctg ggcttcctcg
     5221 acgaaaccca cgatgtcgcg gtcgcggacg ttgagttgca cgtagctcct gagcaggccg
     5281 ttttcgctct tgatcatcga cgggccttcc acaatccgca cgtctgcgat ttgagccagc
     5341 ggaatctgcg cgacgccgtt ggggaaggga ttgagggctg atggcgattc gctcctggcc
     5401 gcgctggtca gcatcgcggc gccattggat ccgccgttgc ccgcgccatt catgccgcgc
     5461 gtaccgccct cgccagccgc catcgccatc gctccgccgg cgctgatgag caggttcttc
     5521 accgattgct cgtcctcgcg gaaggcccgg gcgtagcgaa tccgcacggg aaaccgctcg
     5581 cggccttcga cggtcatcgt gatcatcttg ccgcccaagg cgacttcgac gacgtcctgt
     5641 acgtcgccca cgttgacgcc gtagcgggcc gccttgtcgc ggtcgatgtc gatttcgagg
     5701 tatccttcgc cgacaatctg gtcgggaaaa acgtccgccg cgccgggaac gtcgcgcagc
     5761 accgcggcaa tctgctccga gaccgcttgg atcttgttca ggtcgtcccc aaagaccttc
     5821 accccgatca tcgtccgcac gccc
//
LOCUS       DASUGB010000310         5406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_590121, whole genome shotgun sequence.
ACCESSION   DASUGB010000310 DASUGB010000000
VERSION     DASUGB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590121"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..812)
                     /locus_tag="VFV87_05970"
     CDS             complement(237..812)
                     /locus_tag="VFV87_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103335.1"
                     /translation="MSNDFAALRAQARKRRDNELARVREEYQENLAKIADLEQRLLGR
                     IDPKRMPLTAAVESVLPLGEPFTTTSVMRSLEALDATRVWRMASVMRHIRALRERGLV
                     RRMKRASVHEPAQYIRTDGPAKVVPVPRDRSLRDWLEEIVTKPMRTAEVMQAVKESGF
                     QTTMIAGHLRTYVKRSLREAGYREVAGKWQR"
     gene            complement(809..2227)
                     /locus_tag="VFV87_05975"
     CDS             complement(809..2227)
                     /locus_tag="VFV87_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5103336.1"
                     /translation="MGQARSDPNSQHAQAYAPLVLLIAALAAGIVVDRHWPLTAAIWW
                     GIGAAALVAWLPVWMRGHERVASWLLLGSSLATGGAWHHSYWNSYRADEIGRMVREEI
                     RPIVVEGIAITSPRWIPAPPDTAMRIIPKGEETEVLMWMTGVRDRADWRPASGWALMR
                     VDGVLTGVRSGDKLRVMALGSQPQAPLNPNEFNYAQYERSRRLHCRLRALFPESVERL
                     SRGSPWSPRLWLGQVRDHGNTLLRRHISPQRATLAAAVLIGAREQLDPERNEGFLVTG
                     TIHILSISGLHVGILAAGFWIIFRTGLLPRRSTLLAAMLLTIGYACLTDLQPPVVRAT
                     ILVVAMCLARLTGRQALGFNTLALAGIVVLALNPASLFLAGPQLSFLAVAAMIVFQRV
                     LSPLPTTDPLERLIASTRPWPVRFSKAVAREVWRVWLTGAIIFAISMPLVWKQYNRYC
                     PNETTQYGYNLLPLLARLSLRA"
     gene            2366..3277
                     /locus_tag="VFV87_05980"
     CDS             2366..3277
                     /locus_tag="VFV87_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103337.1"
                     /translation="MVSSVLEVEAGVAGDESRNGEQAEARGEQRGGTAAEGLRELFAK
                     VPPSARDFDVYEHVVVEGGHTRSAAHEHKISQTRVCQVLSRVRRWIREVLGAEAREFT
                     PEQQLALAVAVAADRLDCLYRASMCGWNASTEFEVEKVTTLANGRQITTETSGFGEPR
                     YLIAAGRLALMRAKLPTGGVLTEGLATAAGPDEPLPEEETTDAPPEVDCSDPEEFRQE
                     MLKAVAGARDGSRDVRGSCDPLNEEAKAARRAFFGPVQDNRAAQEHLLQGFAQLLGAK
                     RNGHAVAAMEPGASAVVPIAAQDHLTD"
     gene            3557..4240
                     /locus_tag="VFV87_05985"
     CDS             3557..4240
                     /locus_tag="VFV87_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103338.1"
                     /translation="MPKQPIDPRDVFLAGTNFELASILLAEYDRKQFAAIHDRRKKQG
                     FCNDLVMLMNFPQTVNAAFALELYFKTLIYVESVTPTVDPIREHRLLPLFNLISIPRQ
                     KRIRELFDQLTEHHPWWIRTKTSFPSEFVDLQLLKALENASNAFREHRYYFEGIESAK
                     RIAFGPAVPATRMLILEINPEWRKFLKTVLPTGLDQSSLSSPAGDQRTGGGAATWNSV
                     PPEANIGSE"
     gene            complement(4333..>5406)
                     /locus_tag="VFV87_05990"
     CDS             complement(4333..>5406)
                     /locus_tag="VFV87_05990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5103339.1"
                     /translation="EVMKLPRMAESLKLIAAEGRDAFYKGRIAKTIVAFSEKHGGYFS
                     LADFADHTDDWIEPVSTNYRGYDVWELPPNGQGIAALEMLNVLEQWDLRSLGHNSPEH
                     IHLFVEAKRLAFADRAKFYSDPAFNDLPIKELISKPYGKSQAARINLAKAASDVPAGD
                     PKLAHGDTIYLSVVDKDRNCCSLIQSNYFPFGSKVVPGDVGFVIQNRGALFALEDDHL
                     NRLEPHKRPFHTIIPAFVTKDGKPWFCFGVMGGDVQPQGHVQVLVNLIDFGMNVQAAG
                     DAARTTHTGSATPTGLPAEGVGTVTVESGISDAAVAKLQSLGHRIIRGKSGGGYQGIL
                     IDWENNVLHGATESRKDGAAVGY"
BASE COUNT         1031 a         1678 c         1749 g          948 t
ORIGIN      
        1 cgtcggacgc cgccaagtag agagcgctca cataagcagc aatgcgcgcc tccatgcaga
       61 gcagcggcca cgcctcccga atcggcgcag cgacgtagtt ctgccagtcg aactggccgt
      121 aagggctatc gaatgcttgc ggcaggcttt gcgctagcgc gttcaaggtt tggtcgggac
      181 aatgcgctaa tagtgcattg acagcgggta tcataacgca ccccgctttc cgcagcctac
      241 cgctgccact tcccggccac ttcccgatag ccagcttccc gcagcgaccg cttgacgtac
      301 gtacgcaggt gccccgcaat catcgtggtc tgaaaccccg attccttgac ggcctgcatg
      361 acttccgccg tccgcattgg cttggtgacg atctcctcca gccaatcccg caacgaccga
      421 tcccgcggca caggcactac cttggccggc ccgtccgtgc ggatgtactg cgccggctcg
      481 tgaacactgg ctcgtttcat ccggcgcact agaccacgct cccgcagcgc acggatatgc
      541 cgcatgacgc tggccatcct ccagacgcgc gtcgcgtcca aggcttccag cgaccgcata
      601 acgctcgttg tggtgaatgg ttcgcccagc ggcaagacgc tctccaccgc tgccgtcagg
      661 ggcatccgct tggggtcgat ccggcccagc aagcgctgct ccaagtcggc aatcttggcc
      721 aggttttcct gatactcctc ccggacccgc gccagctcgt tgtcgcgccg cttccgggct
      781 tgggcgcgta aagccgcaaa gtcgttgctc atgcccgcaa cgataggcgg gcgagcaggg
      841 gaagcaagtt gtaaccgtac tgtgtggttt cattgggaca gtaccggttg tattgcttcc
      901 acaccagcgg catggaaatg gcgaaaataa tcgcgccggt caaccaaact ctccaaactt
      961 cgcgggccac cgctttcgag aatcgaacgg gccagggacg cgtactggcg atcaggcgct
     1021 ctagcgggtc ggtcgtgggg agcgggctga ggacgcgctg gaagacgatc atcgcggcga
     1081 cggcgaggaa cgaaagctgc ggcccggcga ggaacaggct ggctgggttc agtgccagga
     1141 cgacgatgcc ggccagggcg agcgtgttaa agccgagagc ttgccgcccg gtgaggcggg
     1201 ccaggcacat ggcgacgacg aggatcgtgg cccgcacgac cggcggctgc aggtcggtca
     1261 ggcaggcata gccgatcgtc agcagcatgg cggcaagcaa agtcgagcgc cgcggcagca
     1321 ggccggtgcg gaagatgatc cagaagccgg ccgcgagaat cccgacgtgc aggcccgaga
     1381 tcgacaggat atggatcgtg ccggtgacca ggaagccttc gttccgctcc ggatcgagct
     1441 gttcgcgggc gccgatcagc accgcggcgg ccagcgtcgc ccgctgtggc gaaatgtgcc
     1501 ggcggagcag cgtgttgccg tgatcgcgga cttgccccag ccacagccgc ggcgaccagg
     1561 ggctgccgcg cgacagccgc tcgacgcttt cgggaaagag cgcccgcaat cggcagtgca
     1621 ggcggcggct gcgctcgtac tgcgcgtagt tgaactcgtt cggattgagc ggcgcttgcg
     1681 gctggctgcc gagggccatc acgcggagct tgtcgcccga gcggacgccg gtcagcacgc
     1741 catcgacccg catcagggcc cagccgctgg cgggccgcca gtcggcccgg tcgcggacgc
     1801 cggtcatcca catcagcact tcggtttcct cgcccttggg gatgatccgc atggccgtgt
     1861 cggggggcgc gggaatccag cgaggcgagg tgatcgcgat tccctcgacg acgatcggcc
     1921 gaatttcctc gcggaccatc cggccgatct cgtcggcccg gtagctgttc caatagctgt
     1981 gatgccacgc gccgccggtg gccagcgaac tgcccaagag gagccacgac gccacccgct
     2041 cgtgcccgcg catccacacc ggcagccacg caacgagcgc tgccgcacca atcccccacc
     2101 aaatcgctgc cgtaagcggc caatggcgat cgaccacgat ccccgccgcc agcgccgcaa
     2161 tcagcagcac cagcggcgcg tacgcctggg cgtgctgaga attcgggtcg gatcgtgcct
     2221 ggcccatggc cccggattct accagcccga gaaatactta aaaaatcttt ttcgtgcttg
     2281 aatgaaaagg accagacgtg tccttttcag ggtgttggat gggcgtgttg acgtttcatt
     2341 catccaatct ctgggaggat cggtcatggt aagcagcgtt ttggaagttg aggcaggcgt
     2401 cgcgggcgac gagtcacgga atggggagca ggcggaggcg cggggggagc agcggggcgg
     2461 gaccgccgcc gaggggctgc gcgagctctt cgcgaaagtg ccgccgagcg ctcgggattt
     2521 cgacgtctac gagcacgtgg tggtggaggg gggacacacg cggtcggccg cgcacgagca
     2581 caaaatctcg cagacccgcg tctgccaggt gctgagccgc gtccgccgct ggatccgcga
     2641 ggtcttgggg gccgaagcgc gcgaatttac ccccgagcag caactggcgc tcgccgtcgc
     2701 cgtcgcggcc gaccggctgg actgtctcta ccgggcgagc atgtgcggct ggaacgcctc
     2761 gaccgaattc gaggtggaaa aggtcaccac gctggccaac ggccggcaga tcacgaccga
     2821 aaccagcggg ttcggcgagc cccggtatct catcgcggct gggcgtctgg cgctgatgcg
     2881 agcgaaactg ccgacgggcg gcgtgctgac cgaaggcctc gccaccgcgg cgggaccgga
     2941 tgagccgctg ccggaagagg agacgaccga cgcccccccg gaagtggact gttcggaccc
     3001 ggaggaattc cgccaggaga tgctcaaagc ggtggctggc gcacgcgacg gaagtcgtga
     3061 cgtgcgaggg agttgcgacc cgctcaacga ggaggcgaaa gctgcccgcc gagcgttttt
     3121 cggacctgtt caagacaacc gcgcggcgca agagcacttg ctccaaggct tcgcccaact
     3181 actgggcgca aaacgcaacg gacacgcggt tgcagcaatg gaacccggcg cgagcgcggt
     3241 cgtgcccatt gctgctcagg atcaccttac agattgatgc cgcccgctca tcctgcccag
     3301 caactggtga cgactgatcg tcgaacccaa acacagcgaa gttcgaagga atccggacca
     3361 gcgtttctcg cagcgcgccc tcccgcagcg cccaccggca aaagcgcgcc gcacgccaac
     3421 tcggattagc attcccctgc gcccgcgacc aacggcgaag ggacacgaaa agtcgaatgt
     3481 ccttagaatc tcctcatatc cgagcccgaa ccacttttag cctgccagcg tcagtcaagt
     3541 agtgggaggg agtctcatgc cgaagcagcc aattgatccg cgagacgtgt ttctcgccgg
     3601 cactaacttt gagctggcct ctatcctgtt ggcggagtac gaccgcaagc agtttgcggc
     3661 catccacgat cggagaaaaa agcaaggctt ctgcaatgac ctcgtgatgc taatgaactt
     3721 cccgcagacc gttaatgcgg cctttgccct ggagttgtat ttcaaaacgc tcatctacgt
     3781 cgaatcggtc acgccgacgg ttgatccaat cagagaacat agactgctcc cgctgttcaa
     3841 cctaattagc attccccgcc aaaagagaat tagggagcta ttcgaccagt tgacagagca
     3901 tcatccgtgg tggattcgca ccaaaacgag tttcccttcg gaatttgttg accttcaact
     3961 actaaaggca cttgaaaacg ccagcaacgc attcagagag caccgctact atttcgaggg
     4021 aattgagtcg gctaaaagga ttgcgtttgg cccagccgtg cctgccacgc ggatgctgat
     4081 tttggaaatt aatccggagt ggaggaagtt tctgaaaact gttttgccga ctggtctaga
     4141 ccagtcttcg ctttcctccc ccgccggcga tcagcggaca gggggcggcg cggcgacgtg
     4201 gaacagcgtg ccaccggaag cgaacattgg aagcgaataa tggatcaagc ttcaatattg
     4261 gtgatcgata atatcgaaac acgatatcat gaatagccca gacctgcgac gatccaacgt
     4321 cgctcgctgt gatcaatagc cgacggccgc tccgtccttg cgggattcgg tcgcgccgtg
     4381 gaggacgttg ttttcccagt cgatgaggat gccctggtag ccgccgccgc ttttgccgcg
     4441 gatgatgcgg tggccgagcg attgaagttt ggcgaccgcc gcgtcggaga tgcccgactc
     4501 gacggtcacg gtgccgacgc cttcggcggg caggccggtg ggggtcgcgc tgccggtgtg
     4561 cgtcgtgcgg gcggcgtcgc cggcggcttg aacgttcatg ccgaagtcga tcaggttgac
     4621 gagcacctgc acgtgccctt gcggctgcac gtcgcctccc atgacgccga agcagaacca
     4681 gggcttgccg tccttggtga cgaacgcggg gatgatcgta tggaacggac gcttgtgcgg
     4741 ctccaggcgg ttgaggtggt cgtcttcgag cgcgaagagg gccccgcggt tctgaatcac
     4801 gaagcccacg tcgccgggca cgactttcga gccaaacggg aaatagttgc tctgaatgag
     4861 cgagcagcag ttgcggtcct tatcgacgac ggacaggtag atcgtatcgc cgtgggcgag
     4921 cttcggatcg ccggcgggca cgtcgctggc cgccttcgcc agattgatgc gggccgcctg
     4981 cgatttgccg tagggcttgg aaatcagctc cttgatcggc aggtcgttga aggcggggtc
     5041 gctgtagaac ttcgcccggt cggcgaaggc gagccttttc gcttcgacaa acaggtggat
     5101 gtgctcgggc gagttgtggc cgagcgaccg caggtcccat tgctccagga cgttgagcat
     5161 ttccagggcg gcgatgccct ggccgttggg cggcagctcc cagacgtcgt agccgcggta
     5221 attggtggaa accggctcga tccagtcgtc ggtgtggtcg gcgaaatcgg cgagcgagaa
     5281 gtagccgccg tgcttctcgc tgaaggcgac gatcgtcttc gcgatgcgtc ccttgtagaa
     5341 cgcatcgcgc ccttcggcgg cgatgagctt gagcgactcg gccatccgcg gcagcttcat
     5401 cacttc
//
LOCUS       DASUGB010000311         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1008448, whole genome shotgun sequence.
ACCESSION   DASUGB010000311 DASUGB010000000
VERSION     DASUGB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008448"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFV87_05995"
     CDS             <1..938
                     /locus_tag="VFV87_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5103340.1"
                     /translation="LASDGTGMLALRGIMGNMIVVSWLYDRAARWTLDRFGIRGKEGL
                     TTIKAADDEEDEDENSEEAAAADKERVADTREVPNRRIYCLDLRVHASAAPYLEEVKR
                     IARENSMQVVGLGLDNWIGGSPKPEQLERYISPTNDTALGPPVEIKSNPQSAIRIPQS
                     DDAARRWYPVIDYSRCTNCMECIDFCLFGVYGVDKVETILVEQPDNCRKGCPACSRVC
                     PENAIIFPQHKTPTIAGAPVVAGSLKIDLSKLFGAPETGESAEQAAIRERDEQLLLAG
                     RAAVGDSVGLPKRHADRPAEPKDELDSLIDALDNF"
     gene            complement(1044..2492)
                     /locus_tag="VFV87_06000"
     CDS             complement(1044..2492)
                     /locus_tag="VFV87_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5103341.1"
                     /translation="MRVVIILTLALSLAAGATSGAAERPNILWLIAEDFSPDLGCYGT
                     KEVSTPNLDALAASGVRYTRCFTTAPVCSASRSAFMTGMYQTTIGAHNHRSHRDDGYQ
                     LPAGVRVLTDWMRDAGYFTANIRELPADLGFRGTGKTDWNFTYQGQPFDSDQWSDLKS
                     HQPFYAQVNFSETHRTFHAPQHADPAKVEIPPYYPDHPVTREDWAKYLDSATELDRKI
                     GLVLDQLDNDGLAESTVVVFFGDHGQAHVRGKQFCYDSGLRVPLIILWPEKFPAPKHF
                     KPGTVDDRLLAAIDLAPTMLAIAGAEKPAKMQGEIFLGDSAGQPRKYVFGARDRCDET
                     VFRFRTVRTDRYRYIHNFTPDRPFLQKNDYKQRQYPVWTLLKELDAEGKLTPLQKFLT
                     APTMPEEELYDVIADPHETVNLATSPEHQAALKELREALDRWIEESNDQGRQLEPPEI
                     AAAKGATKPANDPKSGAKPKAKGAKAKAGTMP"
BASE COUNT          513 a          926 c          901 g          545 t
ORIGIN      
        1 acctggccag cgacggcacg ggtatgctgg ccctccgcgg gatcatgggg aacatgatcg
       61 tcgtctcttg gctttacgat cgggctgccc gctggacact ggaccgcttc ggcatccgcg
      121 gcaaggaggg gctgacgacg atcaaggccg ccgacgatga ggaggacgaa gacgagaact
      181 ccgaggaagc ggctgccgcc gacaaagagc gcgtcgccga tacccgcgag gtgcccaatc
      241 gccgcatcta ctgcctcgat ttgcgagtcc acgcctctgc ggcgccgtac ctcgaagagg
      301 tcaagcggat cgcccgcgag aattcgatgc aggtagttgg gctcggtctt gacaactgga
      361 tcggcggctc gccgaagccg gagcagttgg aaagatacat cagccctacc aacgacacgg
      421 cacttggccc gccggtagaa atcaaatcga atccgcaatc cgcaatccgc attccgcaat
      481 ccgacgacgc cgctcgccgc tggtatccgg tcatcgacta cagccgctgc accaattgca
      541 tggagtgcat cgacttctgc ctgttcggcg tgtatggcgt cgataaggtc gagacgatcc
      601 tcgtcgagca gcccgacaac tgccgcaagg gctgcccggc ctgcagccgg gtctgccccg
      661 agaatgcgat cattttcccc cagcacaaga cgccgacgat cgccggcgcg ccggtcgtcg
      721 ccggctcgct gaagatcgac ctctcgaagc ttttcggcgc gccggaaacg ggcgaatcag
      781 ccgagcaagc cgccatccgc gagcgcgacg agcaactcct cctcgccggc cgcgccgcgg
      841 tcggcgacag cgttggcctc ccgaagcggc acgccgacag gcctgctgaa ccgaaggacg
      901 agctggactc gctgatcgac gccctcgaca atttctaacg gttggatcga gatcggtcgg
      961 ccgatatctc cggagcggag gcggtaaaag attgcgggtg aaagatgtca tcttttacct
     1021 accatctata accacccccg gttctacggc atcgtgccgg ctttcgcctt ggctccttta
     1081 gcttttggct tggccccgga tttcggatcg ttcgcaggtt tggtcgcgcc tttggcggcg
     1141 gcgatttcgg gcggttcgag ctggcggccc tgatcgtttg attcttcgat ccaccggtcg
     1201 agcgcctcgc gcagctcctt gagtgccgcc tgatgctcgg gtgacgtagc gagattcacc
     1261 gtctcgtgcg gatcggcaat cacgtcgtag agctcttcct cgggcatcgt cggcgcggtg
     1321 aggaactttt gcagcggcgt gagcttgcct tcggcgtcca actccttgag cagcgtccat
     1381 accgggtact gccgctgttt gtagtcgttc ttctgcaaga agggccgatc gggcgtgaag
     1441 ttgtggatgt agcgatagcg gtccgtccgc accgtgcgga aacggaagac cgtctcgtcg
     1501 cagcggtcgc gggccccgaa cacatatttc cgcggctggc cggcggaatc gcccaggaag
     1561 atctcgccct gcattttcgc gggcttttcc gcgccggcga tcgccagcat tgtcggcgcc
     1621 aggtcgattg ccgccagcaa gcggtcatcg acagttccgg gcttgaaatg cttgggcgcc
     1681 gggaacttct ccggccagag aataatcaac ggcacgcgca gcccgctgtc gtagcagaat
     1741 tgcttgccgc ggacgtgcgc ctggccgtga tcgccgaaga agacgaccac cgtgctctcc
     1801 gccaagccgt cgttgtcaag ctgatccagc accagaccga tcttccggtc cagctccgtc
     1861 gcgctgtcga gatatttggc ccagtcctcg cgcgtcaccg gatgatcggg ataatagggt
     1921 ggaatctcga ccttggccgg atcggcgtgc tgcggcgcgt gaaacgtgcg gtgggtctcc
     1981 gagaaattca cctgggcata gaacggctgg tgagatttca ggtcggacca ctggtcgctg
     2041 tcgaacggct ggccctgata cgtgaaattc cagtcggtct tgccggtgcc gcgaaagccg
     2101 aggtcggcgg gcagctcgcg gatattggcc gtgaagtagc ccgcgtcccg catccaatcg
     2161 gtcagcacgc gaacgcccgc gggaagctga tagccatcgt cgcggtgcga gcgatggttg
     2221 tgggccccga tcgtcgtctg gtacatcccc gtcatgaacg cgctgcggct ggccgagcag
     2281 accggcgcgg tcgtaaagca gcgggtatag cgcacgccgc tcgcggcgag cgcgtcgaga
     2341 ttcggcgtcg agacctcctt cgttccatag caacccaagt ccgggctgaa atcctccgcg
     2401 atcagccaca agatattcgg ccgctctgcg gctccgctcg tcgcccctgc tgcgagcgaa
     2461 agcgcgagcg tcagtatgat gactacgcgc attgaattca tcctcctaac gcaagtcctt
     2521 acctgtgcca tgccacgcca cgcaatatcc atcttccggt ctatcgcacg caattatgtc
     2581 cggccctggc cagaattcca aagattcttt atgcctggca tcagcaagga tcggcgcggc
     2641 ggaagcgatg gaagcggaac cagagacttc agcctgactg cgacggtttg tggacaactc
     2701 cgtaggacgg ttttccaacc cgtccgccgc cgcaggatag gttggaaacg tgtcgtacca
     2761 ctgtcggccg atcctagcgc cgtcccccac ggccccggcc gccgcggccg cgattgccgc
     2821 cgccccggcc gcggtctcgc tcgcggtcgg gccgatcggc gcgggccgcc atgttggcct
     2881 cgatg
//
LOCUS       DASUGB010000312         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1702567, whole genome shotgun sequence.
ACCESSION   DASUGB010000312 DASUGB010000000
VERSION     DASUGB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702567"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFV87_06005"
     CDS             complement(<1..360)
                     /locus_tag="VFV87_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103342.1"
                     /translation="MSWTNVKLIFFRELRDQLRDRRTLFTIFVLPLMLYPLLGMSVFQ
                     VQQFLKEHASKVRIVGTAGLPEEPKLLADGKFAADLATEYDNRLLELELIADGKQSVT
                     QARDQAEADIRLGTCDAV"
     gene            complement(377..1195)
                     /locus_tag="VFV87_06010"
     CDS             complement(377..1195)
                     /locus_tag="VFV87_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5103343.1"
                     /translation="MIHVRDLTKSYADLRRGQFVALAGISFDALEGQIYGLLGPNGAG
                     KTSALRILSTILRPTSGTATVNGCDVLTQPALVRHQIGFVSANTAVYDRMTAWEMVEY
                     FGRLYGLPPELLRQRMEAIFSRLQMNEIRDLLGAKMSTGMKQKVSIARALVHDPPVLI
                     FDEATAGLDVLVARALLKTVAELRDQGKCVLFSTHIMREAERLCDRMAILHRGHILAE
                     GTLPELRERYAEEDLEELFFGLISQHDELGLNSEFRIQNSEGAPASGHTLHSAF"
     gene            1463..>2594
                     /locus_tag="VFV87_06015"
     CDS             1463..>2594
                     /locus_tag="VFV87_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5103344.1"
                     /translation="MNSFDAPATFATTPDGFPEVPLPLMAAGGQAAPAGVVTPATAPA
                     EFVPAEPSTLDQAGLTPIDIESLVLKLLHISGPTVGRRIAEQVRLPFGLVAEQLRSLK
                     AQLLVNYSNQAAMGDFEYELTEEGGKRAQWHLGRCTYCGAAPVPLEDYIASIRRQSIR
                     NMRPKLPDLCAAFADLLLPPASLSQIGQAVYAGRGLFLYGQPGNGKTSIAERVIRAMS
                     QNIWIPRTITVTGEIIRVFDPANHEEAPLTSPQGQLLDVLHYDRRWVRIRRPSIVVGG
                     ELKLEHLEITNNPITGILESPVQLKSNGGALVVDDFGRQRCSTEELLNRWIVPLEKGH
                     DFLSLPSGRQIEVPFDQLLIFSTNLEPHRLVDEAFLRRIPYKI"
BASE COUNT          459 a          851 c          797 g          487 t
ORIGIN      
        1 gacggcgtcg cacgttccca agcggatatc cgcttccgcc tggtcgcgcg cctgcgtgac
       61 gctctgcttc ccgtcggcga tcagctccag ctccagcagg cgattgtcgt attccgtggc
      121 cagatcggcg gcgaacttgc cgtccgccag cagcttcggc tcttccggca gacccgcggt
      181 gccgacgatc cgcaccttcg aggcgtgctc tttgaggaat tgctggacct gaaagacgct
      241 catccccagc aggggataga gcatcagcgg caagacgaaa atcgtgaaca acgtccgccg
      301 atcgcggagc tggtcgcgca gctcgcggaa gaagatcagc ttgacgttcg tccaggacat
      361 gggggaaatg cagaattcag aatgcagaat gcagagtgtg accggaagca ggcgcgcctt
      421 ctgaattctg aattctgaat tctgaattca gcccgagctc atcgtgctgc gagatcaaac
      481 cgaagaacag ttcttccaga tcttcctccg cgtaccgctc gcgcagctcc ggcagcgtgc
      541 cttcggccag gatgtggccg cgatggagga tcgccatccg gtcgcacagg cgttccgctt
      601 cgcgcatgat gtgggtgctg aagaggacgc acttgccctg gtcgcgcagc tcggccacgg
      661 tcttcagcag ggcgcgggcc acgaggacgt cgagcccggc ggtcgcctca tcgaagatca
      721 gcacgggcgg atcgtgtacc agggcccggg caatcgagac tttttgcttc attccggtgg
      781 acatcttcgc gcccaagagg tcgcggattt cgttcatctg cagccgcgaa aagatcgcct
      841 ccatgcgctg gcgcagcagc tccggcggca ggccgtaaag ccggccgaag tattcgacca
      901 tttcccaggc ggtcatgcgg tcatagacgg cggtgtttgc cgaaacgaag ccgatctggt
      961 ggcgcaccag ggccggctgg gtgagcacat cgcagccgtt gacggtggcg gtaccggaag
     1021 ttgggcggag gatggtgctg aggattcgca gggcgctggt ttttcccgct ccatttggcc
     1081 ccagcaggcc gtagatctga ccttccagcg cgtcgaacga aatgccggcc agggccacga
     1141 attggccgcg gcgcagatcg gcgtaggact tggtcaaatc gcgaacgtga atcatgaaag
     1201 cttcctgcgc tctctcccgg ctgacgtcgg cccgcgcgcg ctccgcttgc cttatatcac
     1261 accctacatc accaaggaat ctgctcggcc acggttcacg gcggcctttt tgcggccgcg
     1321 tccggctgtg ccgattgttc ccgatctatc gccgggcacc cgctatggcg gcgggaaacc
     1381 gcgtcgtgtc atcgcgttgc gagctactct tgtgcgcccg ttttgccgcc ttctcactcc
     1441 tgccaatctg cgagccccgg ccatgaattc gttcgacgcg ccagcgacct ttgcgacgac
     1501 cccggacggc ttccccgagg ttccgctgcc tttgatggcc gcaggcggtc aggctgctcc
     1561 tgctggcgtc gtgacgcctg ccacggcgcc cgccgaattc gtcccggccg aaccctccac
     1621 gctcgatcag gccggcctga caccgatcga tatcgagtcg ctggtgctca agctgctcca
     1681 catcagcggg ccgacggtcg gccgtcggat tgcggagcaa gttcgcctgc cgttcggcct
     1741 ggttgccgag cagcttcgca gcctgaaagc ccagttgctg gtcaattaca gcaaccaggc
     1801 ggcgatggga gatttcgaat acgagctgac cgaggagggg ggcaagcgtg cccagtggca
     1861 tttgggccgc tgtacgtatt gcggcgcggc gcccgtgccg ctggaagact acatcgccag
     1921 catccggcgg cagtcgatcc gcaatatgcg gcccaagctg ccggacctgt gcgccgcctt
     1981 tgccgatctg ctcctgccgc cggcctcgct cagccagatc ggtcaggccg tctatgccgg
     2041 ccgcggtctg ttcctgtatg gccagccggg caacggcaag accagcatcg ccgagcgcgt
     2101 tatccgggcg atgagccaga acatctggat tccgcggacg atcaccgtca ccggcgaaat
     2161 catccgcgtt tttgaccctg ccaaccacga agaggcgccg ctgaccagcc cgcaggggca
     2221 gttgctcgac gtgctgcact acgatcgccg ctgggtccgc atccgccgcc cgtcgatcgt
     2281 cgtcggcggc gaactcaagc tcgaacatct cgaaatcacc aataacccga ttaccggcat
     2341 cctcgaatcg cccgtccagc taaaaagcaa cggcggagcg ctggtggtgg acgacttcgg
     2401 ccgccagcgc tgcagcaccg aagagctgct caatcgctgg atcgtgccac tggaaaaagg
     2461 gcacgatttc ctgagtcttc cgtcgggacg ccagatcgaa gtccccttcg accagctcct
     2521 gattttctcg accaacctgg agccgcaccg cctcgtggat gaggccttcc tccgccgcat
     2581 tccgtacaag atcg
//
LOCUS       DASUGB010000313         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1146453, whole genome shotgun sequence.
ACCESSION   DASUGB010000313 DASUGB010000000
VERSION     DASUGB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146453"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFV87_06020"
     CDS             complement(<1..960)
                     /locus_tag="VFV87_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5103345.1"
                     /translation="MYDLLIQPTINWAKAIGRGWDRFWFAPQQPHTLALIRIFGGGML
                     FYTHLVWSLNLLTFLGAEGWLPASTVAAIKTNEDGSNYAWSYLFYVDSPVLLWILHIG
                     ALVVLAMLTLGLFTRVTSILAFIITLSYCHRLEGSLFGLDQINALIATYLMLGPAGAV
                     YSLDRWLALRRAGNLGRPMPPTLPSVGANIAIRLIQIHMCVIYLFGGIGKARGELWWD
                     GSAMWFAFATLEYQSLDMTWTVRMPWLLAIMTHLTLFWELSYALLIWPRLTRPVMLIL
                     AVLVHGGIALAMGMKTFGLAMIIGNLAFVYPEYVEATVAALSGL"
     gene            complement(1032..1748)
                     /locus_tag="VFV87_06025"
     CDS             complement(1032..1748)
                     /locus_tag="VFV87_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103346.1"
                     /translation="MQKRPAAAEADGVTARMEVSLAPWLKVLLSVLLTAHVVAVVTPP
                     FGFACRSAPGPEGTSPVVNGLAAVFEPYYQALYLDHGYFFFAPTIGPTHLVDYKVEFE
                     DGRPPIEDRFPDLQTERPRLLYHRYFMMAEALNNLYVPPQPPPEPVPPPLTAPDLERL
                     RFREMRRAHPDAVAAWKHRRWQYDSLRTAMEQHLLAAYGGSKVTLTRVEHRQAIPSEF
                     TEQGKQLNDPASYQFLSETQ"
BASE COUNT          418 a          649 c          769 g          366 t
ORIGIN      
        1 gagcccgctg agggcggcga ccgtcgcctc gacgtactcg ggatacacga acgccaggtt
       61 gccgatgatc atcgccaggc cgaaggtttt catgcccatc gccagggcga tgccgccgtg
      121 gaccagcacg gccagaatga gcatgacggg gcgggtcagc cgcggccaaa tgagcagggc
      181 gtaggacagc tcccaaaaga gcgtgaggtg ggtcatgatc gccaggagcc acggcatgcg
      241 gacggtccag gtcatgtcga gcgactggta ctcgagtgtg gcaaaggcga accacatcgc
      301 gctgccgtcc caccacagct cgccgcgggc cttcccgatt ccgccgaaca ggtagatcac
      361 gcacatgtga atctggatca ggcggattgc gatgtttgct ccgacggaag gcagggtggg
      421 aggcatcggc cttcccagat ttccggcgcg gcgcagcgcc agccagcggt cgagtgaata
      481 caccgcgccg gcgggcccga gcatcaggta ggtggcgatg agcgcgttga tctggtccag
      541 cccgaacagc gagccttcca gccggtggca ataggaaagg gtgatgatga atgccaggat
      601 ggaggtcacg cgcgtgaaga ggcccagcgt gagcatcgcc agcacgacca gcgccccgat
      661 gtgcagaatc cacaggagca ccggcgaatc gacgtaaaac agatagctcc aggcgtaatt
      721 cgagccgtct tcgttcgtct tgatcgccgc gacggtcgaa gccggcagcc agccttccgc
      781 tcccaggaac gtgagcagat tcagcgacca gacgaggtgc gtgtaaaaca gcatgccccc
      841 gccgaagatg cggatcaggg ccagcgtgtg cggctgctgg ggggcgaacc agaagcggtc
      901 ccacccgcgg ccgatggcct tggcccagtt gatcgtcggt tggatgagga ggtcgtacac
      961 gggttggctc gtagccgcac tcgccagagt gcgggcacaa cgcttggcag cccgcattct
     1021 ggcgaacgcg gctactgggt ttcggagagg aactggtaac tggccgggtc gttgagctgc
     1081 ttgccttgct cggtgaattc tgaggggatg gcctggcgat gttcgacgcg ggtcagcgtc
     1141 accttcgagc cgccgtaggc ggcgagcagg tgttgctcca tcgcagtgcg cagcgaatcg
     1201 tattgccagc ggcgatgctt ccaggccgcg acggcgtcgg gatgggcccg ccgcatctcg
     1261 cggaatcgga gtcgctccag gtccggcgcg gtcagcggcg gcggcacggg ctccggcggc
     1321 ggctggggcg gcacataaag gttgttgagc gcctcggcca tcatgaaata gcggtgatac
     1381 aggagccgcg gccgctcggt ctgcaaatcg ggaaagcggt cctcgatcgg cggccgaccg
     1441 tcttcgaatt caactttata atcgaccagg tgcgtggggc cgatcgtcgg ggcgaagaag
     1501 aaatagccgt ggtccaggta cagcgcctgg taatacggct cgaagacggc ggccaaaccg
     1561 ttgaccacgg gcgaagtgcc ttccggcccc ggagcgctgc ggcaggcgaa gccgaacggg
     1621 ggcgtgacga ccgccacgac gtgcgcagtc agcaggacgc tcaagagaac cttgagccag
     1681 ggggcgagcg agacttccat gcgcgctgtc acaccgtcag cttccgctgc ggccgggcgc
     1741 ttttgcatgg agaggtctat cgtcgctcga ttggcaggct ggcaaacttt tccgcctggt
     1801 gcggcctggc cggcaaggac ggcagtgcct gcaaagttgt cacatgcgga acgcacgacg
     1861 gagggggcgg gagtcaattg cggtcacgat cttccacatg ggaagttctc agccgcaatt
     1921 gactcccgac ccctttgcgt agcgcacgaa aaagcccgcc ggcgatttcg ccagcgggct
     1981 tcttgagctg aatgggtgag gcgggagccc ctggggagtt ggctcccagg ccggagcccg
     2041 ggaaccagat tggttcaggg aaccgcgacc aatggtcgcg gctttgttac tgagccgacg
     2101 cggttactga gccgaggcga ccttatcgtt ttcgaagtcg aagctcagtt cctgagtctg
     2161 gccgccggtc agcgtgatcg tcttctcctg gacgaccgtg cg
//
LOCUS       DASUGB010000314         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1008677, whole genome shotgun sequence.
ACCESSION   DASUGB010000314 DASUGB010000000
VERSION     DASUGB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008677"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1159
                     /locus_tag="VFV87_06030"
     CDS             293..1159
                     /locus_tag="VFV87_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103347.1"
                     /translation="MMRRPYFLPLLACAAAVSWPSDSSLGQEKYKNFDDAYSQGARLL
                     NRMQIAAAQEPLEAALKLAADDSARLRVYQALVPAYRQLPEIDKMLEAQEFVIRHTPR
                     KAGRSNAANDLASFLHQRGKIDVAIARYEAALKANPKDAAALSVLEKIFTRVKRDGER
                     GPVLTRKLQELDSELAAASAEQLEKDAEAAPRTAAHIYKDAGLAWLEAGDKEKALAAA
                     KKSLASIPESRGEQLVYFWRRGLGDIFLGAGEPAQAIPQFEAALAVVKIYGYRKDTEK
                     KLAEAKEAAKTQ"
     gene            complement(1240..>2372)
                     /locus_tag="VFV87_06035"
     CDS             complement(1240..>2372)
                     /locus_tag="VFV87_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103348.1"
                     /translation="LATAAEYRGLGFASSLVAAAERRGREQGSVLGLVRTRAAPLFAR
                     RGWAVCGRHSFSTAGARQILAQLQSTAEGLDGCRHAGGSDDSRPLDSAYDLLRPAARP
                     AIFLRPLRRIELPAVMRLYEQSLAGRHGSPVRSEAYWEWLLNRGACHRSYVASEGPES
                     SDIKQQLAAIRGAVFIHEGRIVELLVERGRPDIAEHLVARVCGDASELDHWQVRLDAP
                     PDDPVHKLLEAAGGRFHLADELGGEAFMAKALQPRQLLESLGPTLASRLHAAGVPRPA
                     MLGLEIQGGAKEPRSRLPEVARLRLEFTGRGMKLAAGPLGRCYVSLRLKDLAPLVLGH
                     WDLAEALEAERMAASSKQARELAGILFPRLAWWRPPLDDLLA"
BASE COUNT          426 a          802 c          778 g          366 t
ORIGIN      
        1 gtcgattctc ctaggttccc gtggggcgcc tccaccacga gcattcgggg tgaagaagcg
       61 cgacagataa gcatggatgc cgcccgcttg cggacggcga cccaagggag aatggcaaat
      121 tacataccgc tcttgccctg gcccttcttc ccagtggacg atatgccaat ctgccatgcc
      181 gcccgcgccg tttggtcgcc cgcacgctta tgctgtcggc aaccgtacac agcggcggcg
      241 cttgcgggaa ttgagacctg cctttcacca gttgcctctg gagatcacgg tgatgatgag
      301 acgtccctat ttcctgccgc tgctggcgtg cgccgccgcg gtgtcctggc cttccgacag
      361 ttcgctgggc caggaaaagt acaagaactt cgacgatgcc tatagccagg gggctcgcct
      421 gctgaaccgg atgcaaattg ccgctgccca ggagccattg gaggcggcgc tcaaactggc
      481 cgcggacgac agcgcccgcc tgcgggtcta tcaggccctc gtaccggcgt accgccagtt
      541 gccggagatc gacaaaatgc tcgaggccca ggaatttgtg attcgccata caccgcggaa
      601 agcgggccgc tcgaatgctg caaacgatct ggcctcgttc ctccatcagc ggggcaagat
      661 cgacgtcgca atcgcgcggt atgaagcggc cctgaaagcg aatcccaagg atgccgccgc
      721 tctctcggtg ctggagaaga tattcacccg ggtgaagcgc gacggcgagc gcgggccggt
      781 tctgacgcgc aagctccagg agctcgacag cgaattggcg gctgcttcag ccgagcaact
      841 ggaaaaagac gcggaagctg ccccgcgcac ggcggcccac atctataagg acgccggcct
      901 ggcctggctg gaagcggggg acaaggaaaa ggctctcgcg gctgcaaaaa aatcgctcgc
      961 cagcattcct gagagccgcg gcgagcagct cgtttatttt tggcgccgcg gcttgggcga
     1021 catcttcctg ggcgcgggcg agccggccca ggccattccc cagtttgaag cagcgctggc
     1081 ggtcgtcaaa atttacggct atcgcaaaga cacggaaaaa aaactggccg aagccaagga
     1141 agccgcgaag acgcagtaag cgggctattg tcccgcattt ccaaagccaa aagcccgctc
     1201 gaccttggcc gcgcgcagct catagcgggt gtaccactgt taggccagca agtcatccag
     1261 cggcggccgc caccacgcca gccgcggaaa caggatgcct gccagttcgc gggcttgctt
     1321 gctagaggcg gccatccgct cggcttccag ggcctcggcg aggtcccagt gccccagcac
     1381 cagcggcgca aggtccttga gccgcagcga cacgtagcag cgccccagcg gaccggccgc
     1441 gagcttcatt ccacggccgg tgaattccag ccgcaggcga gccacctcgg gaagtcgcga
     1501 gcggggctct ttggccccgc cttggatttc caggcccagc atcgcggggc gcggcacacc
     1561 ggcggcgtgc aggcgggagg caagtgttgg acccaagctt tccagaagtt gacgcggctg
     1621 cagcgccttg gccatgaacg cctcgccccc cagttcgtcg gccaggtgaa agcggccgcc
     1681 ggcagcctcg agcagcttgt ggaccgggtc gtcgggcggg gcatcgagcc gcacttgcca
     1741 gtggtcgagc tcgctggcgt cgccgcagac gcgggcgacc agatgctcgg caatgtcggg
     1801 gcggccgcgc tcgacgagca gctcgacgat ccgcccttcg tggatgaata ccgcgccgcg
     1861 aatcgccgcc agctgttgct tgatgtcgct cgattcaggc ccctcggacg ccacatagct
     1921 gcggtggcac gctccgcgat tgagcagcca ttcccagtac gcctcgctgc gcacgggcga
     1981 gccgtgccgc ccggcgagcg attgctcgta gagccgcatg accgcgggca gctcgatccg
     2041 ccgcagcggt cgcaggaaga tcgccgggcg ggccgcgggc cgcaacaggt cgtaggcgct
     2101 gtccagcggc cgcgaatcgt cgctcccgcc cgcgtgacgg cagccgtcca acccttcagc
     2161 ggtggactgc agctgggcca ggatctggcg ggcgccggcc gtcgagaacg aatgtcgccc
     2221 gcagacggcc caccccctcc gggcaaagag cggggccgcg cgcgtccgca ccaggcccag
     2281 cacgcttcct tgttcgcgcc cgcgccgctc ggccgcggcg accagcgagc tggcaaagcc
     2341 cagcccccga tattcggccg ccgtggccag gt
//
LOCUS       DASUGB010000315         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1146662, whole genome shotgun sequence.
ACCESSION   DASUGB010000315 DASUGB010000000
VERSION     DASUGB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146662"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFV87_06040"
     CDS             <1..1290
                     /locus_tag="VFV87_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103349.1"
                     /translation="TCDRFYTHAAEGVLRVLDAVPEQESLAWRNDVHQLQAALRPILA
                     QTADGPKEREKRVKELHRILVDFVGRLPLVEMRPLVRPATWAWTASLITISFVSLYLA
                     YLHGTVMWELEYAYPFGPPSRTPRAGPYLEVARSIVLLAMALYLLESTLRPEYGRLLL
                     DWSWKSWRMPLVGTFRLFGLASAVLLSWVVGSYILSTAPKNLTVEARQKFEQIKQDKT
                     DVQYLPEHLAALVRESQPGFLGHLEPYKWYLPYSWVNYVGFGLPLLVVTAFSVVGDAQ
                     RLIDERQGLFRVLSNARLPDTEINAAFGTFFDICFHHTQRYITVLAWISGTIAFELIV
                     SKGTLAPAGLMYEFLASAVVGALTLVWIGVLIHFYEGAYQRCCAEKRRRQKLSDEWEH
                     NWRTTKFLYRCFFGHAGGIFAALYIVPFVLNFGRMWT"
     gene            complement(1265..2218)
                     /locus_tag="VFV87_06045"
     CDS             complement(1265..2218)
                     /locus_tag="VFV87_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEX5103350.1"
                     /translation="MPGMLVHHWLALRIWKHPALASMRGNSERETWNAFLCGSLGPDM
                     GMFPGGEPLISDLAHYVRPGDLANLLVDSAGSDVERAFAWGWVAHVLADIALHPLVNR
                     GVGQLLWRDSSREIPYGEDPRAHARVELGLDAAVGSRRADAASFSLSAVFDEGGISYL
                     SAAFGEIYDGQDFAAEFMASQAALVRWSAEWQALAAIAARRFGLPVPQRPVRGLAAWA
                     YWPAWILTAPLSEASAVAGLFRPVPPDKWMLDELDRLADEVERDFVARVVRGQSQAAN
                     INLDTGQVEHPAAPYPLTVRTLARWQARRKRGQATSTSGQS"
BASE COUNT          600 a          983 c          953 g          603 t
ORIGIN      
        1 acgtgcgacc ggttctacac acacgcggcc gagggcgtgc tgcgggtgct ggatgcagtt
       61 cccgagcagg aatcgctagc ctggcggaac gacgtgcatc aattgcaggc ggcactccgc
      121 cccatcctgg ctcagacagc cgacggcccg aaagagcgcg agaagcgggt gaaggagctg
      181 caccggattc tcgtcgattt cgtcggccgc ctgccgctgg tggaaatgcg cccgctcgtt
      241 cggcccgcaa cctgggcgtg gacggcttcg ctcatcacga tcagcttcgt ttcgctctac
      301 ctggcctacc tccacggcac cgtcatgtgg gagctggagt atgcctatcc gttcggccca
      361 ccatcgagaa cgccgcgtgc cgggccgtat ttggaggtcg ccaggagcat tgtcctcttg
      421 gccatggcgc tgtatctgct ggaatcgacg ctccgaccgg agtatggcag gctgctgctc
      481 gattggagct ggaaatcgtg gcgcatgccg ctggtcggca cgttccggct cttcggtctg
      541 gcatctgctg tactgctgag ctgggtcgtg ggcagctaca tcctgtccac ggcgcctaaa
      601 aacctgaccg tcgaggcacg ccaaaagttc gaacaaatca agcaggataa gacggacgtg
      661 caatacctgc cggagcatct ggcggctctg gtgcgggagt cccaacccgg gttcctgggg
      721 catctcgaac cgtacaaatg gtatctgccc tactcctggg tcaattatgt cgggttcggg
      781 ctgccgctgc tcgtggtaac ggctttctcg gtcgtgggtg acgcccagcg gttgatcgac
      841 gagcgccagg gtttgtttcg cgtgctttcc aacgcccggc ttccggacac cgagatcaac
      901 gcggccttcg ggacgttctt cgatatctgc tttcatcaca cgcagcgcta catcacggtt
      961 ctggcctgga tttccgggac gatcgcgttc gagctgatcg tcagcaaagg gacgctcgct
     1021 cccgcggggc tgatgtacga attccttgcg tcggcggtcg tcggcgcgct gacgctggtg
     1081 tggattggcg ttctgatcca tttttacgaa ggtgcttacc agcgctgctg cgccgagaaa
     1141 cgacgccgcc agaagctgag cgacgaatgg gagcacaatt ggagaacgac caagtttctc
     1201 tatcgctgct tcttcggcca cgctggcggg atctttgccg cgctctatat cgttccattc
     1261 gtcctcaact ttggccggat gtggacgtag cttggccccg tttccgcctg gcttgccagc
     1321 gtgcgagcgt gcggaccgtc aaggggtacg gagcggctgg gtgttctacc tggcccgtgt
     1381 cgaggttgat gttcgcggcc tgcgactgac cgcgaacgac tcgcgccacg aagtcgcgct
     1441 ccacttcatc ggccagccgg tccaactcgt ccaacatcca tttgtcaggc ggcaccgggc
     1501 gaaacagccc cgccaccgca ctcgcctcgg aaagcggcgc ggtcaaaatc cacgccggcc
     1561 aataggccca ggcagccagt ccgcggacgg gccgctgggg gaccggcagc ccgaagcgtc
     1621 tggcggcaat ggccgccagc gcttgccact cggccgacca gcgaaccagg gccgcctgcg
     1681 aggccatgaa ttccgcagcg aagtcttgac cgtcgtagat ttcgccaaac gccgcgctca
     1741 agtagctgat gccgccctcg tcgaataccg cggaaagcga gaatgacgcc gcgtcggcac
     1801 gtctcgatcc gacagcagca tccaaaccca actccacgcg cgcatgagcc ctgggatcct
     1861 cgccatacgg aatttcccgc gacgagtctc gccacagcag ctggccgacg ccccgattta
     1921 ccagcgggtg cagggcgatg tcggccagca catgcgcgac ccagccccag gcaaacgcac
     1981 gctcgacgtc ggacccggca gagtccacga gcagatttgc gagatcgccg ggccgcacat
     2041 agtgagccag atcagaaatg agcggctcgc cgccgggaaa cattcccata tccggaccga
     2101 gcgaaccgca caggaaggcg ttccaggttt ctctttcgct attacctcgc atggacgcca
     2161 gcgccggatg tttccagatt cgcagggcca gccagtggtg gaccaacatt ccgggcatgc
     2221 cggtactcca ggtgcaatcg ccgaatcata ttaccgagcg aaagcagccg tacgaaagcg
     2281 caaggacgct tcggtcccat cgattgcggc gcgttgccct cgtgcgactt gaccttgagc
     2341 gcgtcgaagg tcgtaccatt ctggctgtga atggacttgg ccactatgcc gtcgtcccct
     2401 ttcgtgaatt tcacgctggc agcaacaact cgccattcaa actcggtttc ggacttggga
     2461 aaaatcggaa acttgggctg gccggtcagc tgagcaaatc gctgcgcgcc ggcccgtgat
     2521 gccatgatca tctcctgcgc ttaccagctg cgaaactaga cacgggatat tcgctttagg
     2581 ggctggtact gtgaaccgac tcccgatcgt gtcaggctcg acaggacagc gggggtccac
     2641 tgaatgatga gtagtttagc cgagcccgaa ccctcgtgtg actgcagagt gagcccccaa
     2701 tggtgaagag ttttccgaag agcggcaaac tgaatcgccc gaagactgcc gtcaagggca
     2761 agcgataccg ttgcgctctg tgccacaaac gaagtccgaa gccagtgaag actgctgctc
     2821 cttggtattg ctccaaatgc cagaagttga gatcgccggg cgaggcggcg ggagaatcgg
     2881 aactggagta cggcagtcaa tgacggtgga tctcagcatg acttgctctc ggcccctacc
     2941 aatctggccg agggagtagt tcagctaccg gtgccgccta tctctgcgac ggtcggtcga
     3001 tttcaccgct gcgagttggc accattccta tcgcgcggcg gcccgatgca accgaagcgc
     3061 gcttcgtagc ccaacgtaga atgccacagc acccgctgca tcccgtctct gcgacgatgc
     3121 gaaggccaac attccgaag
//
LOCUS       DASUGB010000316         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_35511,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000316 DASUGB010000000
VERSION     DASUGB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35511"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFV87_06050"
     CDS             complement(<1..655)
                     /locus_tag="VFV87_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX5103351.1"
                     /translation="MTPVPPAPAPLLPDPVAEALRGGPEAWTSPQRLSSTLQVMLLLT
                     VLSLAPAILLMTTSFVRIVVVLGLLRQALGTQQLPPSQVMTTLALFMTLLVMGPTWTE
                     IYNASIRPYSQGEITDPEAAFTAGALPLKRFMSRQIELAGNSDDVWLFYEYLPEHQRP
                     ARPPETYDDVPLQVLLPAFMLSELKVAFLIGFQIYLPFLILDLVVSSVTISMGMMMLP
                     "
     gene            complement(766..1428)
                     /locus_tag="VFV87_06055"
     CDS             complement(766..1428)
                     /locus_tag="VFV87_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX5103352.1"
                     /translation="MRYWTHALVTIVAGAVIGAGSAGAQESVYTRHLEARPIPTESPP
                     RSPVRLATAEEAVPIAERGAPLRLMPRSESSSRGIDRPAAPTVNGAIGTVAGSLAIVI
                     GLFLVLAWCSRKFAPAGSTQLPKEALELLGRSSLGPRQQVQLVRIGNKLLLVAITAAG
                     AETLTEITDAEEVERLAALCRRGQAASATSSFTQVMTQLAREPAAGGFAGQSRRATRG
                     AA"
     gene            complement(1450..2088)
                     /gene="fliN"
                     /locus_tag="VFV87_06060"
     CDS             complement(1450..2088)
                     /gene="fliN"
                     /locus_tag="VFV87_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX5103353.1"
                     /translation="MAGESPNARQEDIEELLRQAQMAAGLTAETAPASSAGKGPEGAQ
                     PATSDPPPAAEPAQPGPAVAVAEPPRHSRPAAAPPAAGSMIGDDIQLLIAHAQQAIAS
                     VDRPEDPGIPGLAAFALKDLTGSPASGEKATLELLQDVDLDLRIELGRTRMYLEDVLK
                     LKRGSVVALDKLAGEPVDVYVNGRMVARGEVLVLNDNFCVRVTELVTGDTFE"
     gene            complement(2147..2665)
                     /locus_tag="VFV87_06065"
     CDS             complement(2147..2665)
                     /locus_tag="VFV87_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103354.1"
                     /translation="MSKTIIIAVLVSVVILTECLFAYLLIPSTADIDAWAKAQESGKT
                     EAGEHGEHGEPGEEGNVGEHGKHEEEVDLGKFNVVVHHPSSQVTLRVNFHLIGTVAED
                     DHAEFEELLKKHEHRLRDHTIFEVRNCQIEDLTDPGLALLKRRILAKSNELFGQPMLH
                     SVVFSDFSFVEQ"
     gene            2891..4033
                     /gene="lpxK"
                     /locus_tag="VFV87_06070"
     CDS             2891..4033
                     /gene="lpxK"
                     /locus_tag="VFV87_06070"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5103355.1"
                     /translation="MVAQSWVREILSGHRRGPLAAGLRGVLSVAESVYAWEVRRRNRW
                     FDAGRMKSAEFEAPIISVGNLTVGGTGKTPLVLWLARWLNERGHQVTIISRGYGRKTT
                     APNDEALELAARLPGVPHVQNPNRVAAAREAIGRAFNASAAGGLENRPTHVLLLDDAF
                     QHRRIARDLDIVLLDALEPFGYEHLLPRGLLREPVSSLARADVVALSRADAVAESLRS
                     EIRERVESLAPHALWLELAHQPTALVACSGARQALASWRGRRVAAFCGIGNPAGFRHT
                     LQSCGLGLAGFRELPDHFAYSPQAVSDLETWLRSLDQIAAAICTRKDLVKLPRATLAH
                     VPLLALEVDLEIIRGQQALEDAAKAAIDRKLPPGGHPVAPDERSRG"
     gene            complement(4030..4338)
                     /locus_tag="VFV87_06075"
     CDS             complement(4030..4338)
                     /locus_tag="VFV87_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5103356.1"
                     /translation="MLKRTGHRDGLAIEAGAQGAARQIAAAAKAALDCSQIYMLRKLQ
                     VEPDGKALILSGHVESFYHKQLAQELVRGAAPGAEVVNVIQVVYNRERSLTESDFFRP
                     "
     gene            complement(4510..>4958)
                     /gene="cysC"
                     /locus_tag="VFV87_06080"
     CDS             complement(4510..>4958)
                     /gene="cysC"
                     /locus_tag="VFV87_06080"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX5103357.1"
                     /translation="PQMLAEYGAAFANRFGLGFGSADRQENIRRIGEVANLFASAGIV
                     VLTAFVSPYRADRAVVRKMVTAARPGSFVEVYVDAPLEVCEARDPKGLYKKARAGQIR
                     NFTGIDDPYEAPEMPEVHLQAANRPPQELAKQVLAALEDRHLIIRI"
BASE COUNT          844 a         1592 c         1593 g          929 t
ORIGIN      
        1 gcggcagcat catcatgccc atcgaaatgg tgacgctgga aacgaccagg tccaggatca
       61 aaaacggcag gtatatctga aagccgatca ggaacgctac cttcagctcg ctgagcatga
      121 aggccggcag gagcacttgc agcgggacgt cgtcgtacgt ttccgggggc ctggcgggcc
      181 gctgatgctc gggcaggtat tcgtagaaca gccacacgtc gtcgctgttg ccggccagct
      241 cgatctgccg gctcatgaag cgcttgagcg ggagggcgcc ggcggtgaag gccgcttcgg
      301 ggtcggtgat ttcgccttgc gaataggggc ggatgctggc gttgtagatc tcggtccagg
      361 tcggccccat gaccaagagc gtcatgaaca gggccagcgt cgtcatcacc tggctcggcg
      421 gcagttgttg cgttcccagc gcctgccgca aaaggcccag cacgaccacg atccgcacga
      481 agctcgtcgt catcagcaga atcgccggcg ccaggctcag cacggtcaac agcagcatca
      541 cctgcagcgt ggaactgagc cgctgcgggc tggtccacgc ctccgggccg ccgcgcagcg
      601 cttcggccac cggatcgggc aatagcggcg cgggcgccgg cggcaccggc gtcatcgggc
      661 ccgctacgga ggaagtcgaa ctctgggcgg cggccggcga tgcgatagcg acaaccagtg
      721 acactgcgat caggctggca gcgccgatgt tcttgaacca ttggctcatg ctgcacctcg
      781 cgtcgcgcgc cgcgattggc ccgcgaatcc gcctgcggct ggctcccggg caagctgagt
      841 catgacctgg gtgaacgagc tggtggcgct ggccgcctgg ccgcggcggc agagggcggc
      901 gaggcgctcg acctcctcgg catcggtgat ctcggtcagc gtttcggcgc cggccgcggt
      961 gatggcaacc agcagcagct tgttgccgat tcgcacaagc tgcacctgct gccggggccc
     1021 gagcgacgag cgccccagca actcgagagc ctccttggga agctgagtcg atccggccgg
     1081 tgcgaacttg cgcgagcacc aggccaaaac caggaacagg ccaatcacga tcgccaggct
     1141 tcccgcgacg gtgccgatcg ccccattcac cgtgggagcc gccggccggt cgatgcctcg
     1201 cgaactgctt tcgctgcgcg gcatcaggcg cagtggcgct ccacgttcgg cgatcggcac
     1261 agcctcctcg gctgttgcca ggcgcacggg tgagcgcggt ggggattccg tgggaatcgg
     1321 tcgggcctcc aaatgccggg tgtacaccga ttcctgcgcg ccggctgagc ccgcgccgat
     1381 cactgcgccg gccacaatcg tcacgagcgc gtgggtccag taccgcatcg atactttccg
     1441 tcagccgggt tattcgaacg tatcgccggt gaccagttcc gtcacgcgga cacagaaatt
     1501 gtcgttgagc accagcactt cgccgcgggc gaccatccgg ccgttgacgt acacatcgac
     1561 cggctcgccg gccagcttgt cgagggccac tacgctgccg cgtttcagct tgagcacgtc
     1621 ctccaagtac atgcgcgtgc ggcccagttc gattcgcagg tcgagatcga cgtcctgcaa
     1681 gagctcaagc gtcgccttct cgcccgacgc gggcgaaccg gtcaggtctt tgagcgcgaa
     1741 tgccgccagg ccgggaattc cgggatcttc cggccgatcg accgaggcga tcgcctgctg
     1801 ggcgtgggcg atcagaagct gaatgtcgtc gccgatcatg ctgccggccg caggcggcgc
     1861 agcggcggga cgggaatgcc tgggcggctc tgcgacggcg acggctggac ctggctgggc
     1921 gggctcagcg gcaggcggcg gatctgacgt ggccggttgt gcgccttcag ggcccttccc
     1981 agccgaggat gcgggcgcag tttccgccgt cagcccggcg gccatttggg cctgccgcag
     2041 cagctcctcg atgtcttcct gtcgagcgtt tggcgattcg cccgccatcg gagtcctttc
     2101 cgtcaggggc gtggccgcat ccaagcagcc gcggtttttg gctggcctac tgctcgacaa
     2161 acgaaaagtc gctgaacacg accgagtgga gcatcggctg gccgaaaagt tcgttacttt
     2221 tcgccaaaat ccgccgcttg agcaaggcca atccggggtc ggtcagatcc tcgatctggc
     2281 aattgcggac ctcgaagatc gtgtgatccc gcagccggtg ctcgtgtttt ttcagtagct
     2341 cctcaaattc cgcgtggtcg tcctcggcca cggtgcctat caggtggaaa ttcacgcgca
     2401 gcgtcacctg cgacgacggg tggtggacca cgacgttgaa tttgcccaaa tcgacttcct
     2461 cctcatgttt cccatgctcg ccgacattcc cttcttcgcc aggctcaccg tgttccccgt
     2521 gttcgccggc ctctgttttt cccgattcct gcgcctttgc ccaggcatcg atgtctgccg
     2581 tgctgggaat cagcaggtac gcgaacaggc attccgtcag gatgacgacg ctgaccagga
     2641 ccgcaatgat gattgttttc gacatagctc ggctgacctc gtatgcgagg ctctgttggg
     2701 agtccgcgag tcaattcgat gtgcaattct aggttgcggc aaccgtcgtg gatcctctac
     2761 gatccgtgaa tcgtatcacc gcaagtcctt gccatgtaat aaattgcagc gatctgtttg
     2821 aattggattt cgcagtgctc gcccgtttta gatcgtcggg tggccagtcc aggaaagcca
     2881 caaggaagtc atggtcgctc aatcttgggt ccgcgagata ctcagcggtc acaggcgggg
     2941 tcctcttgcg gcgggcttac ggggcgtctt gtcggtggcg gaatcggtgt atgcgtggga
     3001 ggtgcggcga cgcaatcggt ggtttgacgc agggcggatg aagtcggcgg agtttgaggc
     3061 gccgatcatc agcgtgggca acttgacggt tggcggcacc gggaaaacgc cactggttct
     3121 gtggctggct cgctggctga acgaacgcgg ccaccaggtc acgatcatca gccgcggata
     3181 cggccgcaaa acaactgcgc caaatgacga agcgctggag ctcgcggccc ggctgccggg
     3241 ggttccgcac gtgcagaatc cgaaccgcgt ggccgcggcg cgcgaagcca taggacgagc
     3301 tttcaacgcg tctgcggcgg gcgggctgga aaaccgtcct acgcacgtgc tgctcttgga
     3361 cgacgcattc caacaccgtc gcatcgcgcg cgatctggat attgtcttgc tcgatgccct
     3421 ggagcccttc ggctacgagc atttgctgcc gcggggcctg ttgcgcgagc cggtcagcag
     3481 cctggcgcgg gccgacgttg tcgccctgtc gcgagccgac gcagtcgctg aatcgctccg
     3541 cagcgaaatc cgtgagcgag tcgagtcgct cgcaccgcac gcgctctggc ttgagctggc
     3601 gcaccagccg accgcgctcg tcgcctgcag cggcgcccgt caagccctgg caagctggcg
     3661 tggccgccgc gtggccgcct tctgcggcat cggcaatccc gctggctttc ggcacacgct
     3721 ccagtcgtgc ggcctggggc tcgcgggatt ccgagagctg cccgatcact tcgcctattc
     3781 gccgcaagcc gtctccgacc tcgaaacctg gctccgcagc ctcgaccaaa tcgcagccgc
     3841 gatctgtacg cggaaagacc tcgtgaagct cccgcgcgcg acactcgcgc acgttccgct
     3901 cctggccttg gaggtcgacc tggaaattat tcgcggccaa caggcgctgg aagacgctgc
     3961 taaggcagcg atcgaccgca agctgccgcc gggtggccat ccggttgcgc cagacgagcg
     4021 ctcacgcggc taaggccgaa agaagtccga ctcggtcagc gatcgctcgc ggttgtacac
     4081 gacctgaatg acgttgacga cttccgcgcc cggcgcagcg ccccgcacga gttcctgcgc
     4141 gagctgcttg tgataaaacg actcgacgtg cccgctgagg atcagcgcct ttccatcggg
     4201 ctccacttgc agcttgcgaa gcatgtaaat ctggctgcaa tccagcgccg ctttggcagc
     4261 tgccgcgatc tgtcttgccg cgccttgagc gccggcttcg atggccaacc cgtcccggtg
     4321 gcctgttcgt ttgagcaatg tttcggccat ccttcttcgg ccccccctcc gtggccagcg
     4381 agccgcaata ccgtgagcgg cttgccggca tttgtccgtg gaaatatgca cgtccggaag
     4441 agtcccgaac gctgtccggc aaccgcttca atgtagggca gtcatcctta aagtcaacgt
     4501 caagggaagt taaatcctga taatcaagtg gcgatcttcc agcgctgcca gcacttgctt
     4561 cgctagctcc tggggcggac gatttgccgc ctgtaggtgt acttccggca tctcaggagc
     4621 ctcgtaggga tcgtcaattc ccgtaaagtt cctgatttgc ccggctcgcg cctttttgta
     4681 cagccccttg ggatcgcgcg cctcacacac ctccaagggg gcatcgacat aaacctcgac
     4741 aaagctgccc gggcgagcgg ccgtcaccat tttgcggaca accgcacgat cggcgcggta
     4801 ggggctgacg aacgcggtga gcacaacgat gccggccgaa gcgaagaggt ttgccacttc
     4861 gccgatgcgg cgaatgtttt cctgtcggtc cgcgctgccg aagccgagtc cgaagcgatt
     4921 ggcgaaggcc gcgccatatt cggcgagcat ctgcggtg
//
LOCUS       DASUGB010000317         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1146887, whole genome shotgun sequence.
ACCESSION   DASUGB010000317 DASUGB010000000
VERSION     DASUGB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146887"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..983)
                     /locus_tag="VFV87_06085"
     CDS             complement(255..983)
                     /locus_tag="VFV87_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103358.1"
                     /translation="MKWKALSIVASAALCLASTALAQQRLVVGPEAAPAPVVRRPIFN
                     QISWWAKYGQPVESTVAPNGEPMVLHDHGHGFGYVYGMGSCDCRPPCTDQLWAGYVQN
                     PWRCSHPQYHQRGNCAACGHISDCNCATPTATACGCSDAVGSCGCTDGACCSKCKHKK
                     QWLAHWHWGKKKGCCDSCTSCSVPVSCADPAATAAPGGMAPLPPAPPGAVRLTPPTPL
                     PSGDEASVLKRPLFRQASIGSGLR"
     gene            1321..2247
                     /locus_tag="VFV87_06090"
     CDS             1321..2247
                     /locus_tag="VFV87_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103359.1"
                     /translation="MATISTNAPPFSAEVIATVLSAAAIAVALAIWLSARTAIRETTL
                     VGPWWWALAAIIGWGGVEIAAAGGWLANGAWLAPLRMAAMGLSFCPAVALVGAKRPQH
                     AAWSFVVVSLWVIVVLPAAEGFFLRRGQELEMGDARGWFLWILILLGPANFAPTRFWL
                     AALLLAGGQIVALGKFLPLARGDLVIRPGLTGFVMSVMALLAAWLAAVPFCPRNQIQQ
                     SPYELLWIHFRNAFGLFWSLRVQERVNAAAAQYGWDFRLTWTGLKHNADGQPLTTIDP
                     KAERAFRTTLRGLLRRFVSSRWIADRLQGSLD"
BASE COUNT          393 a          780 c          796 g          461 t
ORIGIN      
        1 aaccccgccc tcccggtact gtcgtattgg tggtatcggc gctggcgggc gtgccgctac
       61 agccgttctc gcgcacgctc gtcgtttaac ggcgagccgc aggcgaagcc gttgcatgct
      121 tgacggcacg ccttcggcta cccgttaaac gaagcatccg ttcgtgcgca aaaaaccagg
      181 cagagcccgc agactctgcc cgatcggtgg attttccaat gtttgctcaa gactgacgca
      241 agccggcacg tcgattaccg cagtccgctg ccaatgctgg cctgacgaaa cagcggcctt
      301 ttgaggaccg aggcttcgtc gccggagggg agcggcgtgg gaggagtcag acgaaccgct
      361 ccgggcggag ccggcggaag aggagccatt ccgcccggtg cggcagtggc cgcggggtcg
      421 gcgcagctaa ccgggacaga gcacgaggtg caggagtcgc agcagccctt ctttttgccc
      481 caatgccagt gggccaacca ttgcttcttg tgcttgcact tggagcagca ggcgccatcg
      541 gtgcagccgc acgagccaac ggcgtcgctg cagccgcagg cggtcgccgt cggcgtggcg
      601 cagttgcagt ccgaaatgtg accgcaagcc gcacagtttc cgcgctggtg atattgcgga
      661 tgcgagcagc gccagggatt ctgcacatac ccggcccaaa gttgatcggt gcagggggga
      721 cggcagtcgc acgaacccat tccatacacg tatccaaaac cgtgtccgtg gtcgtgcaag
      781 accatcggct cgccgttggg ggcgaccgtg ctttcgaccg gttggccgta cttcgcccac
      841 caactgatct ggttgaagat cgggcgccgg accacggggg ccggcgccgc ttcgggaccg
      901 acgaccaacc gctgctgggc cagcgcggtg gatgcgagac acagggccgc gctcgcgaca
      961 atcgagagtg ctttccattt catgattcga atcctcctcg agaactccct tgcatcgggc
     1021 cgaccgctcg ccgccgtcat gttcctgccg ggcgattccg gagagccaac ggactcgccg
     1081 ccgcacaggt gcgagcggac ccaggcaagc atgcccgcct gcagggagcg ggcaaatgcc
     1141 ggcgttgatt tttggcaaca aattgctccc cagcgcgctc aacctgccgc cgatgctgca
     1201 cgtaacacct tgtgagtctg tgacttgcag ctaagatcga caggagattt ccgcttcggc
     1261 aagctctccg ggcgcctgct gagcggagca tgggcctttc ctaatcttcg gaccagaggc
     1321 gtggcaacaa tcagcacgaa tgcaccgcct ttttctgccg aagtgatagc aacggtgttg
     1381 tcggccgcgg cgatcgcggt ggcactcgcc atttggctgt ctgcccgaac ggcgattcga
     1441 gagacgacgc tggtcggccc ctggtggtgg gcgctggcgg cgattatcgg ctggggcgga
     1501 gtggagatcg ccgcggccgg cggctggctc gcgaatggcg catggctcgc gccgctgcgg
     1561 atggccgcca tggggctgag cttttgcccg gcagtggcgc tggtgggggc gaagcggccg
     1621 cagcacgcag cgtggagctt tgtcgttgtt tcgctgtggg ttatcgtcgt cctgcccgcc
     1681 gccgagggat tcttcctgcg gcgcggccag gagctcgaaa tgggcgatgc ccgcggctgg
     1741 tttctgtgga ttctgatttt gctgggaccc gccaacttcg cgcccacgcg gttctggctc
     1801 gcggccttgc tcttggccgg cgggcagatc gtcgcgctgg gcaagttcct gccgctggcg
     1861 cgcggcgatc tcgtcattcg gcccgggctg acgggcttcg tcatgtcggt aatggccctg
     1921 cttgccgcgt ggctggctgc tgtgcccttc tgcccgcgaa atcagattca gcagtcaccg
     1981 tacgaactcc tctggatcca ctttcgcaac gccttcggac tgttctggag ccttcgagtt
     2041 caagagcgcg tcaacgcggc cgctgcgcag tacggctggg actttcgcct tacctggacc
     2101 ggcctgaagc acaacgccga cggccagccg ctgaccacga tcgatcccaa ggcggaacgc
     2161 gccttccgca ccacgctcag gggcctgctt cgccgcttcg tctccagccg ctggattgcg
     2221 gatcggctgc agggcagcct agactgaacg gcctgatgcc tgccgattcc agtcagtccg
     2281 ccccgcgcta ttgccacagc ctgccgggcc tcaatcgact gctggaggcg acgcgcattc
     2341 tggcggtcga gaccgatctc gcaaagattc tggatgcgat cgttcatttg gcctcccagg
     2401 cgttgcgctg cgagaaagca atcctctacc
//
LOCUS       DASUGB010000318         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_590963, whole genome shotgun sequence.
ACCESSION   DASUGB010000318 DASUGB010000000
VERSION     DASUGB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590963"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..774)
                     /gene="def"
                     /locus_tag="VFV87_06095"
     CDS             complement(193..774)
                     /gene="def"
                     /locus_tag="VFV87_06095"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5103360.1"
                     /translation="MQILTYPHPTLRRVSKPLKRVDSELRKTIREMFDLMYEAKGIGL
                     AANQVDIPLRFFLVNLEAKAGEGEELVFLNPVISRPHGGSEVAEEGCLSLPQLYGPVI
                     RPKEVRINAYNLSGQEFEADLSGLFARCVQHELDHLDGVLFPDRMAAGAKADIQSEID
                     VFETEFQSRRNTGQVPSDDEIAKKWADWETRYG"
     gene            1004..1678
                     /locus_tag="VFV87_06100"
     CDS             1004..1678
                     /locus_tag="VFV87_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103361.1"
                     /translation="MLRNGVFGLLSLALFALPALGQEYKVEKVATSAPADELSPEIAA
                     QLQPDGFKVIKGESRTVCEIWLAKELPAKADAKLSTEILYPLTPGQFVGVIRYPRKGA
                     DFRDQEIPSGVYTLRYSQQPVDGAHVGTSLTRDFLALLPAEKDRDPALLDYKALTTAS
                     KATTGTAHPGILSLQKAEDGGEALAIRENPEKEWTLVRFTAKVKQAGGAKDLPMELVV
                     VGIAAE"
     gene            1693..2175
                     /locus_tag="VFV87_06105"
     CDS             1693..2175
                     /locus_tag="VFV87_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103362.1"
                     /translation="MQIANCKLQISAVALVVSAVVAQAEEPASADFKLETIRGKVVFL
                     AEAMQERTGVAAVEEAKDRILALETRSGELIPLWEDVRGRAFRRDERLRKMEVELLVR
                     RYEGSPVVQIIRVFEVAKDGLYEIDYWCDICAIAMYELKECECCQGPIELRKRKSSGK
                     "
     gene            2337..>3020
                     /locus_tag="VFV87_06110"
     CDS             2337..>3020
                     /locus_tag="VFV87_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5103363.1"
                     /translation="MATLPIRLTWFSALLSVAAVGYADDWPQWMGPNRDDIWRETGIL
                     EKFPVGGPKMLWRTPIAGGYSGPAVAAGRVYVTDYVRKSGELANNPGGRTELQGTERV
                     LCLNAATGKEIWKHEYDCPYAISYAAGPRATPTVHAGKVYTLGAEGNLFCFDAAKGTV
                     LWSKELKKEYQTESPIWGFCGHPLVDGKKLICLVGGKGSVGVAFDKDTGQELWRALSN
                     PDAGYCPPTI"
BASE COUNT          597 a          882 c          999 g          542 t
ORIGIN      
        1 cgccaaaacg gccgcgtacg atggatcatg gacgcgttgt aaatttcagg acaccctcaa
       61 gcagaagctt gggaacgcaa gtcctcgaag ctccgcttcg cgatcatcaa aacagctctc
      121 gcgcctcaag ggaagcagga gcttcccaga cgcgggttcc caagcggagc ttgggaacca
      181 ggagtctaag cttcatccgt agcgcgtctc ccagtcggcc cacttcttgg caatttcatc
      241 gtcgcttggg acttgccccg tgttccgccg gctctgaaac tccgtctcga aaacatcaat
      301 ctccgactga atgtcggctt tcgcgccggc ggccatccgg tcggggaaca ggacgccgtc
      361 caggtgatcc agctcgtgct gcacgcagcg ggcgaagagg ccggacaggt cggcttcgaa
      421 ctcttggccc gagaggttgt aggcgttgat ccgcacttcc ttggggcgga tgacggggcc
      481 gtaaagctgc ggcaggctaa ggcagccttc ctcagctact tctgagccgc cgtgtggccg
      541 cgagatgacg ggattgagga agaccagctc ctcaccttcg ccggccttgg cctccagatt
      601 gacgagaaag aaccgcagcg gaatatcgac ctggttggcc gccaggccga tccccttggc
      661 ctcgtacatc aggtcgaaca tctcgcggat ggtcttgcgc agctcagaat cgacccgctt
      721 cagcggcttg ctgacgcggc gaagtgtcgg gtggggatag gtgaggatct gcaacggcgg
      781 aggggacagg ggacaggaga caggagacgg gcgacaggga attataggtg gatcagcggc
      841 gaggccgtag ggcggtcaat gcgtggcgca ggtattcttg cctgccagca gataggcaga
      901 caggaatgtc tgccccaccg gggccgtttg tcggagccgg gcgagcgacc tatactgaca
      961 tctgcccacc cgctcgcatc ctcccttggt agagaggctc atcatgcttc gcaacggcgt
     1021 tttcggtttg ctctcgctcg cgctcttcgc cctgcccgct cttgggcagg aatacaaggt
     1081 agaaaaggtt gccacatcgg ccccggctga cgagctttcg cccgaaatcg ccgcccaatt
     1141 gcagcccgat gggttcaagg tcatcaaggg cgagtcgcgg acggtctgcg aaatctggct
     1201 ggcgaaggaa ctgcccgcca aagccgacgc caagctctcg accgagattc tctatccgct
     1261 gacgcccggg caattcgtcg gcgtgatccg ctatccgcgg aagggggccg attttcgcga
     1321 tcaggagatc ccctcgggag tctatacgct ccgctacagc cagcagccgg tcgatggggc
     1381 ccacgtcggc acgtcgctga cccgcgattt cctggccctc ttgccggccg agaaggaccg
     1441 cgacccggcc ttgctcgatt acaaagccct gacgaccgcc agcaaggcaa cgaccggcac
     1501 ggcccacccc ggcatcctct cgctgcagaa ggcggaggat ggcggcgagg ccctggcgat
     1561 ccgcgagaac cccgagaagg aatggacgct cgtgcggttc acggccaagg tcaagcaagc
     1621 gggcggggcg aaggacttgc cgatggagtt ggtggtggtg ggcatagcgg cggaatgaga
     1681 ggcaattgaa aaatgcaaat tgcaaattgc aaattgcaga tttcggcagt cgcgctggtc
     1741 gtctcggcgg tcgtcgcgca ggctgaggag ccggcttccg cggatttcaa gctcgagacg
     1801 attcgtggga aggtcgtttt tctggccgaa gcgatgcagg agcggacggg cgtggcggct
     1861 gtggaggaag cgaaggaccg catcctggcg ctggagacca ggagcggcga actcattccg
     1921 ctgtgggaag acgtccgcgg ccgggcattc cgccgcgacg agcgcttgcg gaaaatggag
     1981 gtcgaattgc tcgtgcggcg ctacgagggc tcgccggtcg tgcagatcat ccgcgtcttc
     2041 gaggtcgcca aagacgggct ctacgaaatc gactactggt gcgatatttg tgcgatcgcc
     2101 atgtatgagc tgaaggaatg cgagtgctgc caggggccga tcgagctgcg gaaacgcaag
     2161 tccagcggca aatgaaggaa tgaaggaggg agccacggat gaaacacgga tcgaacacgg
     2221 atgtagaaga ggcgttcgtt ctcgtccgat ccgtgcctat ctgtgtttca attggcgaac
     2281 gcttcccgaa gcgcgtatct gcatcaacca aactcacgtc ttcaatgtga atccgaatgg
     2341 caacgcttcc tatccgcctg acctggttct cggcgcttct atcggtcgca gccgttggct
     2401 atgccgacga ttggccgcag tggatggggc cgaatcgtga cgacatctgg cgggaaacgg
     2461 gcatcctcga aaaattcccc gttggtgggc cgaagatgtt gtggcgcacg ccgatcgccg
     2521 gcgggtatag cgggccggcg gtggcggcgg ggcgggtgta tgtcaccgat tacgtccgta
     2581 agtcgggcga gctggccaat aaccccggcg gccgcaccga gctgcaagga accgagcgcg
     2641 tcctctgcct gaatgccgcc accggcaaag aaatctggaa gcacgaatac gactgcccct
     2701 atgcgatttc gtacgcagcc gggccacggg ccacgccgac cgtgcacgcc ggcaaggtct
     2761 atacgctggg agccgagggg aacctgttct gcttcgacgc ggccaagggg acggtcctct
     2821 ggtcgaagga gttgaagaag gaataccaga ccgagtcgcc catctggggc ttctgcgggc
     2881 atccgctggt ggatggcaag aagctgatct gcctggtcgg cggcaagggc tcggtcggcg
     2941 tcgccttcga caaggatacg ggccaggagc tgtggcgggc gctgtccaat cccgatgccg
     3001 gctattgccc gcccacgatc
//
LOCUS       DASUGB010000319         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_870291, whole genome shotgun sequence.
ACCESSION   DASUGB010000319 DASUGB010000000
VERSION     DASUGB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870291"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFV87_06115"
     CDS             complement(<1..768)
                     /locus_tag="VFV87_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5103364.1"
                     /translation="MGGTNCADFSANPQSAIRNPQFADPAAWLHAETGCDAIAAEQTC
                     RYVAAQQAAVGMVPTGDQILFERFFDESGGMQLVIHAPLGSRINKAWGLALRKRFCRS
                     FDFELQAAADDNGVVLSLGPQHSFPIDSLFGMLTPQNGQHLLEQAVLAVPMFQVRWRW
                     NVTRALAVLRQQGGKRVPPFLQKFRSEDLLAAAFPETVGCLENHHGDVVVPDHPLVQQ
                     TMHDCLHEAMDLDRWLNLLEGIKSKSVQLVPIETREPS"
     gene            946..2094
                     /locus_tag="VFV87_06120"
     CDS             946..2094
                     /locus_tag="VFV87_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5103365.1"
                     /translation="MRTHALVGAGAATAWLEQDIPGREPERIELDKFPFTMGRNESCD
                     RQVLSSRVSREHAEIVREGNGFVVRDNSSTNGTFLNGKKIDSSKLKDGDLLVIADVQF
                     SFRCSGGEGARITVTQVMDPVGDEASGSEEDAAGDLIFAIRQAHETLLHRATRNRFQP
                     VIGLADNRCVGYEALARPQVPGDASLAQQMLAATDCRLTERMSQLHRLVAAEHVARLP
                     SATLLFVNLQPTEVGADALPQSLVALQRLAGGKRVVAEIPDSAVVDIPFFRDFLARLK
                     ELAIDVAYDGFAGSAQQLKSIEKFGPQYLKLAPVLVRGVDKSTQRQQQIRALVEAGTS
                     IGTQLIAVGVHTENEARTCRELGCRLAQGDHFGRPQTIDWPVEGFSLA"
     gene            2206..2397
                     /locus_tag="VFV87_06125"
     CDS             2206..2397
                     /locus_tag="VFV87_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6800 family protein"
                     /protein_id="HEX5103366.1"
                     /translation="MGGTIERHKEIRKRRHRKKKLKTFKRRLAKATISEKAHIATKLR
                     RLTPGAEVIIANLGLEERK"
     gene            complement(2481..3257)
                     /locus_tag="VFV87_06130"
     CDS             complement(2481..3257)
                     /locus_tag="VFV87_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5103367.1"
                     /translation="MTVTVKVNLPSGTIILNRPDKRNALSRQLIADLVQALDDLHGER
                     RVRAVILTGSGTAFCAGMDLGEMQETSKQPDAMSQWHEDAVIYRELIDKMLLFPKPII
                     AAVNGPAVAGGAGLVLASDIVIAAAEAKIGFPEPRRGIVAGMVSPLLVFRIGASHAAN
                     LLLTARTIEAAEAQRIGLYHEIVSSDLIWARANEIAGEIAQSAPEALLLTKKLLNETI
                     GEHLGTLLSAGAAASATSRTTEAAREGLAAFLEKREPKWL"
     gene            complement(3254..3667)
                     /locus_tag="VFV87_06135"
     CDS             complement(3254..3667)
                     /locus_tag="VFV87_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103368.1"
                     /translation="MTHGKTISVRDLASARSGDKGNHANIGVVANDEAAYGILCSRLT
                     AVRVQTHFQELGVSRVERYELPKILALNFVLYDALAGGASRSLRIDTQGKLLATSLLD
                     LTFHANAWEEEEGERGRRGEGESNPQSAIRNPQSP"
     gene            complement(3664..>4873)
                     /locus_tag="VFV87_06140"
     CDS             complement(3664..>4873)
                     /locus_tag="VFV87_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX5103369.1"
                     /translation="DILRSLTPALKQQPRLKIIANSGGMNPPACVRAAATILNEAGLG
                     ETPIACVTGDDLLPRLDELIAAGCNFANFDTGESLHSAIRNPQSEIASANAYLGARPI
                     ADALAAGTRIVVTGRVADASLTVGPAMHEFGWKWDDWHKLAAASVAGHLIECGAQVTG
                     GYSVDWSQFDLAHVGYPIAELSPDGSCVITKPPVTGGAVNRRTVVEQLVYEIGDPQHY
                     LTPDVDCDFTTVEVAETGPDRVVVRGATGRPATDSYKVSLAYRDGWMASGQLLVYGPD
                     CVEKAKDCGEIIRHRCKLERRTFRDDQFLVELLGYGAGVPGAWFWRKYQTPGEIVLRV
                     TARHEQREAVECFSRQFAPLITSGPAGLAGYAAGRTQVRPVFAYWPTLVPKSLVQPQV
                     EVRSAKEWNV"
BASE COUNT          850 a         1607 c         1525 g          891 t
ORIGIN      
        1 ggagggctcg cgcgtttcga tcggcacgag ctggaccgac ttcgacttga tgccttccag
       61 cagattgagc caccgatcca ggtccattgc ctcgtgcagg cagtcgtgca tcgtctgctg
      121 cacgagcgga tgatcgggca cgaccacgtc gccgtggtga ttctccaggc agccaaccgt
      181 ctccgggaac gccgcggcca gcaagtcctc gctgcggaac ttctgcagga acggcggcac
      241 ccgcttgccc ccctgctgcc gcagcacagc cagcgcgcgg gtgacattcc accgccagcg
      301 gacctggaac atcggcacgg ccagcacggc ttgctccagg aggtgctgcc cgttctgcgg
      361 cgtgagcatg ccgaacaggc tgtcgatcgg aaagctgtgc tggggcccta gcgagaggac
      421 gacgccgttg tcgtccgccg cggcctgcaa ttcaaaatcg aagctgcggc agaagcgctt
      481 gcgaagggcg aggccccagg ccttgttgat ccgcgagccg agcggcgcgt ggatcacgag
      541 ctgcatcccg cccgattcgt cgaaaaaccg ctcgaacagg atctgatcgc ccgtcggaac
      601 catgccgaca gccgcctgct gggcggcgac gtagcggcag gtctgctcgg cggcgatcgc
      661 atcgcagccg gtttcggcgt gaagccaggc cgctgggtca gcaaattgcg gattgcggat
      721 tgcggattgc ggattggcgg aaaaatcggc gcaattcgtc ccgcccatcc tctgtcctct
      781 cttctgtcct tctgatccct gtcgcctgtc ccctgtcccc tggcttctca agctatgttt
      841 tcgatggctt attcggccca acaggcgccg cggctggacg ttcgtcctgc cgcccgcccc
      901 tcgacccctg ccgcggatcc gccgcggacg acccctggag ccatcatgcg tactcacgct
      961 ctcgtcggcg ccggcgccgc cacggcctgg ctggaacaag acatcccggg ccgcgagccc
     1021 gaacggatcg agctcgacaa gttccccttc acgatgggcc gcaacgagtc gtgcgaccgg
     1081 caggtgctct cgtcgcgcgt ctcgcgcgag catgccgaga tcgtccgcga gggcaacggc
     1141 tttgtcgtcc gcgacaacag cagcaccaac ggcacgttcc tcaacggcaa gaaaatcgac
     1201 tcttctaagc tcaaggacgg cgacctgctc gtcattgccg acgtgcagtt cagtttccgc
     1261 tgcagcggcg gcgaaggagc gcggatcacc gtgacgcagg tcatggaccc ggttggggac
     1321 gaggccagtg gcagtgaaga ggacgccgcc ggtgatctga tcttcgccat ccggcaggcg
     1381 cacgagaccc tcttgcatcg cgcgacgcgc aaccgcttcc agccggtgat cggcctggcc
     1441 gacaatcgct gcgtgggcta cgaagcgctg gcccgcccgc aagttcccgg cgatgcctcg
     1501 ctcgcccagc aaatgctggc cgcgaccgac tgccgtctga cagaacgcat gtcgcaactg
     1561 caccgcctcg tcgcagccga gcacgtcgcc cggctgccga gtgcgacact cctgttcgtc
     1621 aacctgcagc cgaccgaagt gggcgccgat gccctgccgc agtccctggt ggccctgcag
     1681 cggctggccg gcggcaagcg ggtagtcgct gaaattcccg atagcgccgt ggtcgatatt
     1741 ccgtttttcc gcgacttcct cgcccgtctc aaggagctgg cgatcgacgt ggcctatgac
     1801 ggcttcgccg gctcggccca gcagctcaaa tccatcgaga aattcggccc gcaatacctc
     1861 aagctcgctc ccgtgctggt ccgcggcgtc gataagagca cccagcggca gcagcagatt
     1921 cgggccctgg tcgaggctgg aacctccatc ggcacgcaac tgatcgccgt tggcgttcac
     1981 accgaaaatg aagcccgcac ctgccgagag ctcggctgcc ggctggctca aggcgaccac
     2041 tttggccggc cgcaaacgat cgactggccg gttgagggat tcagtctggc ataggcacat
     2101 ttttcctgca caatctttgt cccacttgtc cgcccccggc agaacgtggt aacttgttcg
     2161 gtttcaacaa gttacagcca agctgctcgg gagagatttc cagtcatggg tgggaccatc
     2221 gaacgccaca aggagattcg caagcgccgt caccggaaga agaagctcaa gacgttcaag
     2281 cggcggctgg cgaaggctac catctccgaa aaagcccaca tcgcgacgaa gctccgaagg
     2341 ctcacgcccg gcgcggaagt gatcattgcc aatctcgggc tggaagagcg gaagtagcag
     2401 cgggaacttc caagaaccaa atcagtcgag acggctaagt ccttggttct tgtatcttcg
     2461 gttcttggta tttcgactcg ctacagccac ttcggctcgc gcttctccaa aaacgccgcg
     2521 agcccttcgc gggcagcttc cgtcgtgcgg gacgtggcgc tggcggcggc tccggcgctg
     2581 agcagcgtgc ccaggtgctc gccaatcgtc tcgttgagca gcttcttggt cagcaagagc
     2641 gcctcgggag cgctttgggc aatctcgccg gcgatttcat tcgcgcgcgc ccagatcaga
     2701 tcgctgctga caatctcgtg atagaggccg atccgctggg cctcggcggc ttcgatcgtt
     2761 cgcgccgtga gcagcaggtt cgccgcgtgt gacgcgccga tccgaaatac gagcagcggc
     2821 gaaaccatgc cggcgacaat cccgcgccgc ggctccggaa agccgatctt ggcctctgct
     2881 gccgcgatga cgatgtcgct cgccagcacc agccccgcgc cgccggcgac cgccgggccg
     2941 ttcaccgccg caatgatcgg cttgggaaac agcagcatct tgtcgatcag ttcgcggtag
     3001 atcacggcgt cttcgtgcca ctggctcatg gcatccggct gcttgctcgt ttcctgcatt
     3061 tcgcccaggt ccatccccgc gcaaaacgcc gtcccgctgc cggtgagaat caccgcccgt
     3121 acgcgccgct cgccgtgcag gtcgtccaac gcctgcacca ggtccgcaat caactgccgc
     3181 gagagggcgt tgcgcttgtc cggccggttg agaatgatcg tgccggaagg gaggttgact
     3241 ttgacggtga cggtcatggc gattgcggat tgcggattgc ggattgcggg ttcgactccc
     3301 cctcgcccct tctccctctc tccccctctt cttcctccca cgcgttagcg tgaaacgtca
     3361 gatccaagag cgacgtcgct aacagcttcc cctgcgtgtc gatccgcagg ctgcggctgg
     3421 cgccgccggc gagggcgtcg tagaggacga agttgagggc gaggatcttg ggcagctcgt
     3481 agcgctcgac tcgcgagacg cccaactcct gaaagtgcgt ctgcacgcgg accgccgtca
     3541 gccggctgca gaggatgccg tacgcggctt cgtcgttggc gaccacaccg atattggcgt
     3601 ggttgccctt atcgccgcta cgggcgctgg ccaggtcgcg aacagagatg gtcttgccgt
     3661 gggtcataca ttccattcct tggcggatcg aacttcgact tgcggctgga cgagcgattt
     3721 gggcacgagc gtcggccagt aggcgaacac gggccgcacc tgcgtccggc cggcggcgta
     3781 gcccgcaagg ccggcggggc cgctggtgat gagcggggcg aattgccggc tgaagcattc
     3841 aaccgcttcg cgctgctcat gccgagcggt gacgcggagc acaatctcac cgggcgtctg
     3901 gtatttccgc cagaaccacg ccccaggaac tccggcgcca tagccgagca actcgaccag
     3961 gaattgatcg tcgcgaaacg tacgtcgctc cagcttgcaa cggtggcgaa tgatctcacc
     4021 gcagtccttc gccttctcga cgcagtccgg cccataaaca agcaactgcc cgctcgccat
     4081 ccagccgtcg cgataggcca gggagacttt atagctgtcg gtggcgggcc ggcccgtggc
     4141 cccgcgtacg accactcgat cgggtccggt ttctgcgact tccaccgtgg tgaaatcgca
     4201 atcgacgtcg ggtgtcagat agtgctgtgg atcgccgatt tcgtagacaa gctgctcgac
     4261 gaccgtccgg cgattgaccg cgcctccggt cactggcggc ttcgtaatga cgcagcttcc
     4321 atctggcgac aattcagcga ttgggtagcc tacgtgagcc agatcgaatt gcgaccaatc
     4381 gacgctatag ccgccggtaa cttgtgcccc acattcaatc aagtgaccgg cgacactggc
     4441 ggcggcgagc ttgtgccagt cgtcccactt ccagccgaat tcgtgcatgg ccgggccgac
     4501 cgtcagcgag gcatcggcaa cgcggcccgt gacaacaatt cgcgttccgg cggcgagagc
     4561 gtcggcgatc ggacgtgctc cgagataggc gtttgcggag gcaatttcgg attgcggatt
     4621 gcggattgcg gaatgaagcg attcgcccgt atcgaaattg gcaaagttgc agccggcggc
     4681 gatcaattca tccagccgcg gcagcaggtc gtcgcccgtg acgcaggcga tcggcgtttc
     4741 gcccaggccc gcttcgttca ggatggtcgc ggcggctcgt acgcaagccg gtggattcat
     4801 gccgccggaa ttggcgatga tcttcagccg cggctgctgt ttcagggccg gcgtcaggct
     4861 gcgcaggatg tcg
//
LOCUS       DASUGB010000320         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1424890, whole genome shotgun sequence.
ACCESSION   DASUGB010000320 DASUGB010000000
VERSION     DASUGB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424890"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1286
                     /locus_tag="VFV87_06145"
     CDS             276..1286
                     /locus_tag="VFV87_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5103370.1"
                     /translation="MSWRLAWLVSLLLLAGCDSAGVSNPPALQADATAFAGPYPIQGV
                     ATVGMVADVVRSVGGEHVQLTQLMGPGVDPHLYMATRDDVSTIMEGDIVFYSGLMLEG
                     KMADTLVKVARTKPVFAVTELIDPRELLEPEDFAGHYDPHVWMDVSAWSTCALAVANA
                     LGEFDPTHAGDYEKNAAAYRQQLAELHQYGLKSIATIPEGSRVLITSHDAFNYFGRAY
                     GLEVQGVQGISTESEAGLQRINELVAMIVAKKVKAVFVESSVPRKNIDALVEGAQSKG
                     HDVVIGGELFSDAMGQSGTYEGTYVGMLDHNITLVTRALGGEAPEAGFQGKLSSLAGA
                     KP"
     gene            1283..2110
                     /locus_tag="VFV87_06150"
     CDS             1283..2110
                     /locus_tag="VFV87_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5103371.1"
                     /translation="MIAATHSPSDTPAARKSGDDLTAVPLSVYDLTVAYHRKPVIWDV
                     SFDIPAGSLVGIVGPNGAGKSTLLKAVMDLVPRASGRISVFGKPYRQNRHRVGYVPQR
                     ESVDWDFPIDALDVVTMGLYSKVGWCLPVRKQHREAALEALRRVGIADLAQRQISQLS
                     GGQQQRTFLARALVQAADLYLMDEPFASVDAATERAIVEILRELKNEGKTAVVIHHDL
                     QTVPEYFDYVVLLNMRVVAHGPVADVFTPENLHKTYGGRLTLLEEVSEAMRRRERSL"
     gene            2107..3597
                     /locus_tag="VFV87_06155"
     CDS             2107..3597
                     /locus_tag="VFV87_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX5103372.1"
                     /translation="MSRTRFCAALSFLICVHVACVAVAQPRIRVQTQGSIADRSIEWP
                     TWNQWRRVLLLEDYNTRVVLLGTTLLGGAAGMVGSFTLLRKRALMGDALSHATLPGIG
                     LAFMFVTAAGGNGKSLANLLLGATISGLVGVGGILLIRNLTRIKEDAALGVVLSVFFG
                     AGLALLSVAQRMPEGNAAGLEGFIYGKTASMVSQDAWLIAAAAVACIFVCLLLFKEFK
                     LLCFDEGFAGSRGFPIVSLDLALMALIVIVTIVGLQAVGLILMIALLVIPAAAARFWT
                     ESMGRMVAVSAVLGAAGGQFGAALSALFSKLPSGAMIVLVCAAFFVFSLIFGSARGVV
                     IRGLRRARLNRNVERQHLLRAIYEALESSDRLSAGGLSLLPAVEIADLLPRRSWSQTQ
                     LLREIRRAERDRLVERAAGGVRLTQNGMGEAARLTRQHRLWELFLITHADIAASQVDR
                     AADDIEHVLEPEVIAELESLLAKSAADIPASPHKIEATSSETPAAL"
     gene            3599..>4774
                     /locus_tag="VFV87_06160"
     CDS             3599..>4774
                     /locus_tag="VFV87_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5103373.1"
                     /translation="MSSDLSLLAKWNWALDGWIVLAGCLCAVAAALLGNFLVLRRMSM
                     LGDAISHAVLPGLAAAFFLTHSRSSLPMFLGAVVVGVLTAFFTEWIRRIGKVDEGASM
                     GVVFTSLFALGLVLIVQTADKVDLDAGCVLYGAIEVTPLDTVSIAGREIPRAVLTLGI
                     VAAINALFVLAFYKELKISSFDPELATTTGFPAGRMHYLLMILVAVTAVASFESVGNI
                     LVVAMFIVPPAAAYMLTDRLPRMIVLSVILAVSSAVLGHLGAITVPMWFGYRSTTTAG
                     MMAVAAGLLFAAAVVFGPKHGILVKLIRRRMLAASILVEDIIALLYRIEERGGQTRAS
                     PQSLREMLLADRLSLRIALTWLRLRREIAVADDAYRLTPSGRDRARGLVRSHRLWEQY
                     "
BASE COUNT          815 a         1477 c         1527 g          955 t
ORIGIN      
        1 tggctgccct ggccgaaaag ccctcgctga ccgaccgcta ccaaattccc gccgtcgtgg
       61 gcctgatggt ggtgggcctg atcttatacg ccgtatttcg ccgactggaa ggaaatgcgc
      121 cgtcagggta gttagcacgt caacggcaaa tttacctatc tgccagttga ctcgcagcag
      181 tcgaatgccg atactatttt tgagcaatca aaaagaactt ttggcacaat agaaattgcg
      241 attggcggag attgagtctt acgggaggac cgccggtgag ttggcgattg gcgtggcttg
      301 tttctctatt gctgctcgcg ggatgcgatt cagccggcgt atccaatccg cccgcgctgc
      361 aagcggatgc gaccgcgttc gccgggccat atcccattca aggggtcgcc acggtcggta
      421 tggtcgcgga tgtggtccgc agcgtaggcg gcgaacatgt tcagctgacg cagctcatgg
      481 ggccgggggt cgatccgcat ctgtacatgg cgacgcgcga cgacgtgagc acgatcatgg
      541 agggagacat cgtcttctac agcggcctga tgctcgaagg gaaaatggcc gacacgctgg
      601 tcaaggtggc ccgcaccaag cccgttttcg ctgtgaccga gctgatcgat ccacgcgaac
      661 tgcttgagcc ggaggacttc gctgggcact acgacccgca cgtatggatg gacgtctcgg
      721 cctggtcaac ctgcgccctt gcggtggcca acgccctggg cgaattcgat ccaacgcatg
      781 ctggcgacta cgagaaaaat gccgccgcct atcggcagca gcttgccgaa ctgcatcaat
      841 acggcctcaa gtccatcgcg acaattcccg agggaagccg ggtgctgatc acttcgcacg
      901 acgccttcaa ctacttcggc cgggcctatg gcctggaagt gcaaggcgtg cagggaatct
      961 cgactgaatc cgaggcgggc ttgcaacgga tcaacgagct tgttgcgatg atcgtggcta
     1021 agaaggtcaa ggccgtcttc gtcgagagca gcgtgccccg aaaaaacatc gatgcgctcg
     1081 tggaaggcgc gcagtccaaa ggccacgacg tggtgattgg cggcgagttg ttctccgacg
     1141 caatgggcca gtccgggaca tacgaaggaa cctacgtggg gatgctcgat cacaatatca
     1201 cgctcgtcac ccgcgccttg ggcggcgagg cgccggaggc gggttttcag ggcaaacttt
     1261 cttccctcgc cggcgccaaa ccatgattgc cgccacccac tcgccttccg acactccagc
     1321 ggctcgaaaa tcgggcgacg atctcaccgc cgttccgctg tccgtctatg acctgaccgt
     1381 cgcttatcac cgtaaaccgg tgatctggga cgtgagcttc gatatccccg ccggttcgct
     1441 ggtgggaatc gtcgggccca acggcgcggg caaaagcacg ctgctgaagg cggtgatgga
     1501 cctggtgccg cgggcgtccg ggcggatcag tgtgttcggc aagccgtacc ggcagaaccg
     1561 ccatcgcgtg ggctatgtgc cgcagcggga gagcgtcgat tgggattttc cgatcgatgc
     1621 gctcgatgtg gtgacgatgg gcctctacag caaagtcggc tggtgcctgc cggttcgcaa
     1681 gcagcaccga gaagcggctt tggaggccct gcggcgcgtc ggcatcgctg atctggccca
     1741 acgccaaatc agccagctct ccggcggcca gcagcagcgg acgttcctgg cccgcgcgct
     1801 cgtccaagcc gccgacctgt acctgatgga cgagccgttc gcttcagtcg atgcggcgac
     1861 ggaacgggcg atcgtcgaga tcctccgcga gctgaagaac gaggggaaga cggcagtcgt
     1921 cattcatcac gatctgcaaa cggtccccga gtacttcgac tacgtcgtgc tgctcaacat
     1981 gcgggtcgtc gcgcacgggc cggtggccga tgtgttcacg cccgaaaacc tgcacaagac
     2041 gtacggcggc agactgacgc tgttggagga ggtgtcggaa gccatgcggc ggagggagcg
     2101 ttcgctgtga gccgcacccg cttctgtgcc gccctcagct tcctgatttg cgtgcacgtt
     2161 gcctgcgtcg cagttgctca gccgcggata cgcgtgcaga cgcagggatc cattgccgat
     2221 cgctcgatcg agtggccgac gtggaaccag tggcggcgcg tgctattgct ggaagactac
     2281 aacacgcgcg tagttttgct cggaacgacg ctactgggcg gtgcggcagg aatggtcggc
     2341 agtttcacgc tcttgaggaa gcgtgccctg atgggcgacg ccctcagcca cgctacgctc
     2401 cccggcatcg gcctggcctt tatgttcgtc accgccgccg gcggcaacgg caagtccttg
     2461 gccaatctgc tattaggagc caccatctcg ggcctggttg gcgtcggtgg aatcctgctc
     2521 atacgcaatc tgacgcgaat caaggaagac gctgcgctgg gcgtagtgct gagcgttttt
     2581 tttggagccg gcctggcgct gctcagcgtc gcccaaagaa tgccggaggg aaatgcagcc
     2641 gggctcgagg gcttcatcta cggcaagacc gcatccatgg tgtcgcaaga cgcctggctt
     2701 atcgccgctg cggcggtggc ctgcatcttc gtctgcctgc tgctgtttaa ggagtttaaa
     2761 ctgctgtgct tcgatgaagg cttcgccggc tcgcggggct ttcccatcgt cagcctcgat
     2821 ctggcgctga tggccttgat cgtcatcgtc acgattgtcg gcctgcaggc ggtggggctg
     2881 atcttgatga tcgccctgct cgtcatcccc gcagccgccg cacgcttctg gaccgaaagc
     2941 atgggacgga tggtggccgt ctcggctgtg ctcggcgcgg cgggcgggca gttcggcgcc
     3001 gcactgagcg cactattctc caagctccct tcgggcgcga tgatcgttct ggtctgcgcg
     3061 gcatttttcg tgttcagcct gatctttggc tcggcacggg gcgtcgtcat ccggggcctt
     3121 cgccgggcac gcctgaatcg caacgtcgag cgccagcatc tcctgcgggc gatctatgaa
     3181 gcgctcgaat cgtccgatcg attgtccgca ggcggtctgt ccttgttgcc ggcagttgaa
     3241 atcgccgatc tcttgccccg gcggagctgg tcgcagacgc agctcctgcg cgagatccgc
     3301 cgcgccgaga gagatcggct tgtcgagcga gcggcagggg gcgtgcggtt gacgcagaac
     3361 ggcatgggcg aagcggcccg gctaacgcgg cagcatcgcc tgtgggagct gttcttgatt
     3421 acccacgccg acatcgccgc cagccaggtc gatcgcgctg cggacgacat cgagcacgtg
     3481 ctcgagcccg aagtgatcgc ggagcttgag tcgctgctgg cgaaatccgc ggccgacatt
     3541 cccgccagcc cgcacaagat cgaggccaca agcagcgaga cgcccgctgc gctttgacat
     3601 gtcttccgac ctttcattac tggcgaaatg gaattgggcc ctcgacggct ggatcgtcct
     3661 ggccggctgc ctgtgcgccg tggcggccgc cctcctgggc aatttcctcg tgctccgccg
     3721 gatgagcatg ctgggcgacg ccatcagcca cgccgtcctg cccggcctcg ccgccgcttt
     3781 cttcctcacc cacagccgca gcagtctccc gatgtttttg ggggcggtgg tcgtgggcgt
     3841 cttgactgcc ttcttcaccg aatggatcag gcggatcggc aaagtggacg aaggcgcgtc
     3901 gatgggtgtt gtcttcacgt cgctcttcgc cctcgggctg gtcctcatcg tccagacggc
     3961 ggataaagtc gatctggatg ccggctgcgt gctctacggt gcgatcgaag tcacaccgct
     4021 ggatacagtt tcgatagcag gccgcgaaat tcctcgcgcg gtcctaacgc tggggatcgt
     4081 cgcggcaatc aacgcgctgt ttgtattggc gttttacaag gagctaaaga tcagctcgtt
     4141 cgatccggaa ctggcgacca ccaccggctt cccggcgggc cggatgcact atctgctgat
     4201 gatcctcgta gccgtcaccg cggtcgccag cttcgaatcg gtcggcaata tcctcgtcgt
     4261 cgcgatgttc atcgtcccgc ccgcggcggc atacatgctc accgaccggc tgccgcgaat
     4321 gatcgtcctg agtgtcattc tggcggtgag ttccgctgtg ctgggccatc tcggcgcgat
     4381 caccgtcccg atgtggttcg gctaccgcag cacgacgacg gccggcatga tggccgtcgc
     4441 tgccggcttg cttttcgcgg cggcggtcgt atttgggccg aagcacggca ttctcgtcaa
     4501 gctcattcgt cgccgcatgt tggcggcaag cattcttgtg gaagacatca tcgcgctgct
     4561 ctaccgcatc gaagagcgag gcggccagac gcgggccagt ccccaaagcc tccgcgaaat
     4621 gctcctggcc gatcgacttt ccctgcggat tgctctcacc tggctccgcc tgcgccggga
     4681 aatcgcggtg gccgatgacg cgtatcggct cacgccttcg gggcgcgatc gcgcccgcgg
     4741 gctggtgcgt tcgcatcgcc tctgggaaca atac
//
LOCUS       DASUGB010000321         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1009417, whole genome shotgun sequence.
ACCESSION   DASUGB010000321 DASUGB010000000
VERSION     DASUGB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009417"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFV87_06165"
     CDS             <1..961
                     /locus_tag="VFV87_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5103374.1"
                     /translation="EFKLQVALAEAQLTQARSALGMKDSDLVDQLNPLGAPPVREARA
                     VLDEAHTRTERLRPLRERNAVTEEVFDAAVAAEQVAEARYTSAYNSVLEKIAQIRVRA
                     AELALAKQRLIDAVILAPFEGFVHQRHVSPGSYVQIGDPVVTLVRTSTLRFQGTLPER
                     HALRLAVGQEVRLAIESIPEPRIAKITRISPVIAEQSRSLMFEAAVDNVDGSLQTGLF
                     AEAEVIVDPQAQSIAIPLSAVVEFAGAEKVWKLVDGIAQEQIVETARRGSDAVEIVSG
                     LEKGDTILLRAAEGRIAHVQPTNSTSVAKPVAVIPDADLPGGE"
     gene            991..>2912
                     /locus_tag="VFV87_06170"
     CDS             991..>2912
                     /locus_tag="VFV87_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5103375.1"
                     /translation="MYWLAELCVRRPVFALMIVMAFVVAGLASFPQLGVDRFPNMDLP
                     TVYVRTAYPGAASTEVESEVSQVLEDAVATVSGIEELRSISSDGQSFVLVTFKLTRNL
                     DAAAQDVRDAVGSVVNRLPRGLDPPVVQKQDTDSSPIITLAVSGPRSPSELYLMADRY
                     VKNVIESAPGVGQVTIAGAADRAVQVDIDARRLAAHKLSILQVREALVAQNTEVPGGR
                     VDEGMRERALRTLGRVPHAREFRDLVVATVGGTAVRLSDLGEVHDATKEVRTLARLNG
                     TPAVVLQVQRQSGENTVEVIQGVKDRLPRSRLLLPQDVQVTVIQDQSRYILAALHEIE
                     GHLVSGSILACITVLIFMRSWRSTLIAAVAIPASIVATFAFMRLFGFTLNNVTMLGLV
                     LMVGVVIDDAIVVLENVFHCIEEKGMDPFQASIEGTREIGPAVLATTLSLVIVFLPVS
                     FLSSVTGRMLFEFGITATVAIMVSMLVSFTLTPMMCSKLLRKGMANPSAPASRRGFYH
                     WIEVGYMACLRFSMRWRFVVLILAILTIAANWPLYSLVKQDYIPTNVDESEFEISIYA
                     REGASLTSMDEALQRVERELRDMQGVDFVLTTVGTRGFGGVNRGELYVRLEDFESRTF
                     SLGRLWRSLLAGDPQAA"
BASE COUNT          482 a          951 c          942 g          537 t
ORIGIN      
        1 ggaattcaag ctgcaagtcg ccttggccga agcccagctt acgcaggccc gctcggcgct
       61 gggtatgaag gacagcgacc tcgttgatca actcaatccg ctaggcgccc cgccggttcg
      121 cgaggcgcgg gcagttctgg acgaagctca cacgcggacc gagcggctcc gcccgctgcg
      181 cgagcggaat gccgttacag aagaggtctt tgacgccgct gtcgctgccg aacaggtcgc
      241 cgaagcccgc tacacgtccg cttacaatag cgtcctggaa aaaatcgctc agattcgcgt
      301 ccgtgcggcc gagttggctc tcgccaagca gcggctgatc gatgccgtga tcctggcgcc
      361 tttcgaggga ttcgttcacc agcggcatgt ttcgccggga tcgtacgtgc agatcggcga
      421 tccggtcgtc acgctcgtgc ggaccagcac gctgcggttt cagggaacgc ttcccgagcg
      481 gcacgccttg cggctggccg tcggccagga agtgcgtctg gcaattgagt ccatccccga
      541 gccgcggatc gccaagatca cccgaatcag ccccgtcatc gccgaacaga gccgctcgct
      601 gatgttcgag gcggccgtcg ataacgtgga tggcagcctg cagacaggcc tgtttgccga
      661 agccgaggtc atcgtcgatc cgcaagcgca gtcgatcgct atcccgcttt cagccgtggt
      721 cgagttcgcc ggcgcagaaa aggtctggaa gctagtggat ggcatcgccc aggagcagat
      781 cgtcgagacg gcccggcgcg gcagcgacgc tgtcgagatc gtgagcggcc tcgaaaaggg
      841 cgataccatt ttgcttcgcg ccgccgaagg cagaattgcg catgtccagc ccacgaactc
      901 cacctcggta gccaagccgg ttgccgtcat tcccgacgcc gacctccccg gcggcgaatg
      961 aattctgacc cctgacccct cgcccgctcc gtgtactggc ttgccgaact ctgcgtccgc
     1021 cgcccggtct tcgccctgat gatcgtgatg gccttcgtcg tcgctggcct ggcgtcgttt
     1081 ccgcagctcg gcgtcgatcg ctttccgaat atggacttgc cgacggtcta tgtccgcacg
     1141 gcatatccgg gggcggcttc gacggaagtc gaatcggagg tttcgcaggt cctggaggac
     1201 gccgtcgcca ccgtcagcgg catcgaggag ctgcggtcca tctccagcga cggccagtca
     1261 ttcgtgctcg tcacgttcaa gctcacgcgg aatcttgacg cggcggccca ggacgtgcgc
     1321 gacgccgtgg ggagcgtcgt caaccgcctg ccgcggggcc tcgacccgcc ggtcgtgcag
     1381 aagcaggaca ccgattcgtc gccgatcatc acgctggctg tctccgggcc gcgttcgccg
     1441 agcgagctgt acctgatggc ggaccgctac gtgaagaacg tcatcgaatc ggcgcccggc
     1501 gtcggccagg taacgatcgc cggagccgcg gaccgggccg tgcaggtcga tatcgacgcc
     1561 cgccgcctgg cggcgcacaa gctttcgatt ttgcaggtcc gcgaggccct ggtcgcgcag
     1621 aacacggaag ttcccggcgg acgcgtcgat gaagggatgc gcgagcgggc cctgcggacg
     1681 ctcggccgcg tgccgcatgc ccgcgagttc cgcgacctgg tcgtggccac cgtcggcggc
     1741 acggcggtgc ggctgagcga tttgggtgaa gtgcatgacg cgacgaagga ggtccgcacc
     1801 ctggcccgcc tgaacggcac gccggccgtc gtgctgcagg tccaaaggca gtcgggagaa
     1861 aacacggtcg aagtcatcca aggcgtcaag gaccggttgc cgcggtcgag actcctgctg
     1921 cctcaggacg tgcaggttac ggtgattcag gaccaatcgc ggtacatcct ggccgccctg
     1981 cacgagatcg aaggccacct cgtcagcggc agcatcctgg cctgcatcac cgtgctcatc
     2041 ttcatgcgct cctggcgctc cacgctgatc gccgccgtcg ccattccggc ttcgattgtc
     2101 gccacgttcg ccttcatgcg gctattcggc tttacgctca ataacgtgac gatgctcggt
     2161 ctggtgctga tggtgggcgt ggtgatcgac gatgccatcg tcgtcctgga aaacgttttc
     2221 cactgcatcg aagagaaggg aatggacccg ttccaggcgt cgatcgaagg gacgcgcgaa
     2281 atcggcccgg ccgtgctggc gacgactctg tcgctggtca tcgtgtttct gcccgtttcg
     2341 ttcttgtcca gcgtcaccgg ccggatgctg ttcgagttcg gcatcaccgc gaccgtcgcg
     2401 atcatggtgt cgatgctcgt cagcttcacg ctcaccccca tgatgtgcag caagctgctg
     2461 cggaagggga tggccaaccc cagcgcgccc gcgtcgcgcc gcggcttcta tcactggatc
     2521 gaggtgggct acatggcctg cctgcgcttc tccatgcggt ggcggtttgt cgtgctgatc
     2581 ctggcgatcc tgacaattgc cgccaactgg ccgctctaca gcctggtcaa gcaggactac
     2641 atcccgacga acgtcgatga atcggaattc gaaatcagca tttacgcccg cgaaggggcc
     2701 agcctgacct cgatggacga agccctgcag cgagtcgagc gcgagctgcg cgacatgcag
     2761 ggggtcgatt tcgtcctcac gaccgtcggc acccgcggct tcggcggcgt gaaccgcggc
     2821 gagttgtatg tgcggctgga ggatttcgaa tcccggacgt tctccctcgg ccggctgtgg
     2881 cgaagcttgc tcgccggcga tccgcaggcg gc
//
LOCUS       DASUGB010000322         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1981700, whole genome shotgun sequence.
ACCESSION   DASUGB010000322 DASUGB010000000
VERSION     DASUGB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981700"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFV87_06175"
     CDS             complement(<1..646)
                     /locus_tag="VFV87_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103376.1"
                     /translation="MITTRHTNLELEFVKWLLLIVAAVALILALATAVAADPMTEPRL
                     LPGDMGAIAQPLPTEPMRRPFFPEAPPASEFPPAEAAARLWMMLQHHQAGETAAALAG
                     WEQLRLPEHTAHWREIAMAAALLRAGDVQQAALHLDLAQNLAPDHPLVAYYTGLMRLE
                     QAADAGRVPDGYDLGQTRWCAYTPIEDKAMYEMIARTELEMAIARAGDVRLDEPL"
     gene            complement(847..1881)
                     /locus_tag="VFV87_06180"
     CDS             complement(847..1881)
                     /locus_tag="VFV87_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5103377.1"
                     /translation="MTTQERLENVLRSLPRMTIGLVGDLFLDRYLECVPGSYEPSIET
                     GLEAFQIERVRNAAGALGTVMNNLAALGAGLLVPVSAIGDDGHGYDLLKEIRKLPVDP
                     AHILCLPERLTPTYTKPMKLDSSGTRQELNRLDVRTREPLSDAATAELCRRVETVFQS
                     SDGLIVLDQLVAENQGVVNSAVRATLSELAGQHPQKLIYIDSRQFLGKFDFGTLKGNQ
                     AEILQACDERETGDAAVVRSLQRLSQKTGHAAFCTIGERGILVAHPASEPQLVPGYPA
                     SGPIDIVGAGDAATSGIVSALLAGASELEAATLGNLVASITVQQLGTTGTATPDQVSA
                     RWREACGKRV"
     gene            2196..3305
                     /locus_tag="VFV87_06185"
     CDS             2196..3305
                     /locus_tag="VFV87_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103378.1"
                     /translation="MSRVLHWGCLLSLVLTAGLAQAADSPKGLDQVGKPDLKSAGPLA
                     FGPKGVLFVGDPQGAAIFAIGVGDAAAAPLSGEFKLDNVQAKIAGLLGTEAADVLLND
                     IAVEPGTNIAYVSVSRGRGPSAEPAIIRVDGKGGLSALPLDKVQYAKVAVSGAPAADA
                     RDKKGSSLRSESITDLAFVDGRLFIAGLSNEEFASKLRAIEFPFKGSDTSTSVEIYHG
                     AHGAFETRSPVRTFVPFNVGGEPHLLAAYTCTPLVSFPISDLRAGQKIRGTTVAELGN
                     RNRPLDIISYEKDGKQYLLLANNARGVMKIATEGIEKQQGITEPIRGGGKAGLNYDTI
                     ADLTGVEQLDRLGDAHALLLVKTGDALNLEAVALP"
     gene            3411..>3800
                     /locus_tag="VFV87_06190"
     CDS             3411..>3800
                     /locus_tag="VFV87_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103379.1"
                     /translation="MSHQTLLSIALLSVAAFCAAAESPIRVVADNDGKIIGFEGTGLP
                     PNSLQGLDKTAPDDPYWPGLFAVYVGTKPGRPDLPPMLGNYALHGSNVRFTPKFPLKP
                     GLSYCVEYFPPPARDIDSPARYEQVFAI"
BASE COUNT          667 a         1212 c         1208 g          713 t
ORIGIN      
        1 gcagcggctc atccaggcga acgtcgccgg cccgggcgat cgccatctcc agctcggtgc
       61 gggcgatcat ctcgtacatc gccttgtctt cgatcggcgt ataagcgcac cagcgagtct
      121 ggcccaggtc atagccatcc ggcacgcgcc ccgcgtcagc cgcctgctcc agccgcatca
      181 agccggtgta gtaggcaacc agtggatggt ccggggccag gttctgggcc agatcaaggt
      241 gcaacgccgc ttgctgcacg tcgccggccc gcagcagcgc ggcggccata gcgatttctc
      301 gccaatgggc cgtgtgctcc ggcaggcgaa gctgctccca accggccagt gccgccgcag
      361 tttcgcccgc ctgatggtgc tgcagcatca tccatagacg cgcggccgcc tcagcgggcg
      421 ggaattcact ggccggtgga gcttctggga agaatggccg tcgcattggc tcggtcggca
      481 gcggctgggc gatcgcgccc atatcgccag gcaagagccg cggctcggtc atcggatccg
      541 cggcgaccgc cgtcgccagc gccaggatca acgctacggc tgctacgatc aagagtagcc
      601 actttacaaa ctcaagctcc agatttgtgt gacgtgtggt aatcacggcc cacctccttc
      661 ccagaaagcg agatttcggc gtccacctgg aagaaaggca aaggccgtgc ccgccgccgg
      721 aatgcgtcaa ttcatgcagc tttcggggca tgccacagca cctttgggag gctggggagg
      781 agtttgccgg cggcgccggc cgaggtcaac cgtgccctgc cgcacagccg tgtgcagtgg
      841 ggcgcgctac acacgcttcc cgcaggcctc acgccaccga gcgctcacct gatctggcgt
      901 tgcggtccct gttgtgccga gctgctgcac cgtgatcgac gccacgaggt tgccgagcgt
      961 agcggcttcg agctcgctag cgccggcgag gagcgcgctg acgatgccgc tagtggcggc
     1021 gtcgcccgcg ccaacaatgt cgatcggacc gcttgccgga tagccaggaa caagttgcgg
     1081 ctcgcttgcg ggatgagcga ccaggatgcc acgctcgccg atcgtgcaga atgccgcgtg
     1141 gccggtcttc tgcgacagcc gctgcagcga gcgaaccacg gctgcatcgc ctgtttcgcg
     1201 ctcgtcgcac gcctgcagaa tctcagcttg attgcccttc agcgtgccga agtcgaattt
     1261 gccaagaaac tgccggctat cgatatagat cagcttctgc ggatgctggc cggcaagttc
     1321 agagagcgtc gctcggacgg cagagttcac tactccttgg ttctcagcga cgagctggtc
     1381 gaggacgatc agcccgtccg aagattgaaa gaccgtttcc acgcggcggc aaagttccgc
     1441 cgtcgcggcg tccgaaagcg gttcgcgggt ccgcacatca aggcggttca gttcctgccg
     1501 cgtgccactc gagtcgagct tcatcggctt ggtataggtc ggcgtcagtc gctccggcag
     1561 gcacaggatg tgtgccggat cgacgggcag tttccggatt tccttgagca aatcatagcc
     1621 gtggccgtcg tcgccaatcg cgctcaccgg cacgagcaac cccgcgccga gcgcggccaa
     1681 attattcatc acggtgccca gcgcccccgc ggcgttgcgc acccgttcga tctgaaaggc
     1741 ctccaatccc gtctcgatcg acggttcgta actgccaggc acacattcca agtagcgatc
     1801 gaggaacaga tcgcccacca ggccgatcgt cattcgcggc agcgagcgga ggacgttttc
     1861 gaggcgctct tgcgtggtca tggtcaactc ggtggataca cgcttgtgta tttggcaggg
     1921 cgacccagag caatccggcg gacggcagaa tttttggaca aaaatacttg ccaggggtgg
     1981 tgattgtggc tgcgccgcgc agtatcgtac tgagtgccgg aaaagatcgc ttggggtggt
     2041 tggcccgctg cgcaagcggc ggagccgatg gcggattcgt gccgactgcc ggccgccggt
     2101 ttgactggtg ggatcggcag tcggcgcgca ccccgccgcc actttgttcg ctcatcgtct
     2161 ctcgttccat cgaatctctg acggaggatt ggatcatgtc tcgcgtattg cactgggggt
     2221 gcttgctgtc gctggtgctg acagccggat tggcgcaagc cgccgattcg ccgaagggtc
     2281 tggaccaggt cggcaagccc gacctgaagt cggccggccc gctggccttt gggcccaagg
     2341 gtgtgttgtt cgtgggcgat ccgcaaggag cggccatttt cgcgatcggc gtgggcgatg
     2401 ccgccgcggc cccgctctcg ggagaattca agctggacaa tgtgcaagcc aaaattgccg
     2461 gactgcttgg gacggaagcg gctgacgtgc tgcttaacga cattgccgtg gagccgggca
     2521 cgaacatcgc ttatgtgagc gtcagccgcg gccgcggccc cagcgccgaa ccggcaatca
     2581 tccgcgtcga cggcaagggc ggcctctcgg ccctgccgct ggacaaggtg caatatgcca
     2641 aggtggcagt gtccggcgcc ccggcagccg atgcccgcga caagaaggga agcagcctgc
     2701 ggagcgaatc catcaccgac ctggcgtttg tcgatgggcg gctgttcatc gctggcctgt
     2761 cgaatgagga attcgccagc aagttgcgag cgatcgagtt cccgttcaag ggttccgaca
     2821 cctcgacgag cgtcgagatc tatcacggcg cccacggagc gttcgagacc cgctcgcccg
     2881 tgcgaacctt cgtgccgttt aacgtcggcg gcgagccgca cctcctggcg gcctatacct
     2941 gcacgcctct cgtctcgttc ccgatttcgg atctcagggc cggccagaag attcgcggca
     3001 cgacggtcgc cgaactcggc aaccgcaacc gcccgctgga tatcatcagc tacgagaagg
     3061 acggcaagca gtacctcctc ctggcgaata acgcccgtgg ggtgatgaag atcgcgacgg
     3121 aaggcatcga gaaacagcag ggaattaccg agcccatccg tggcggcggc aaggccggct
     3181 tgaattatga cacgatcgcc gatttgaccg gcgttgaaca actcgatcgg ctgggtgacg
     3241 cgcacgctct gttgctcgtc aagaccggcg atgcgctcaa cctcgaagcc gtggcgctgc
     3301 cgtagcgatg ggtctcctga ttttggagcc acggatgaaa cacggatgag catggattgg
     3361 agggcctccc cagtccgtgt ttcctcggtg ttccatccgt ggcaagcttt atgtcccacc
     3421 agacgctgct ttcgatcgcg cttctctctg tggctgcgtt ctgcgccgcg gccgagtcgc
     3481 ccatccgagt cgtcgccgac aacgatggca agatcatcgg ctttgaggga actggcctgc
     3541 caccgaattc cctgcaaggc ttagacaaga cagctcccga cgatccttac tggcccggcc
     3601 tgtttgctgt ctacgtcggc accaaacctg gccggcccga cttgccgcca atgctcggca
     3661 actacgcgct ccatggttcg aacgtgcgct tcacgccgaa gttcccgctc aagcccgggc
     3721 tgtcgtattg cgttgagtat tttccgccgc cggctcgtga tatcgattcg ccggcgcggt
     3781 acgagcaggt cttcgctatc
//
LOCUS       DASUGB010000323         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1009806, whole genome shotgun sequence.
ACCESSION   DASUGB010000323 DASUGB010000000
VERSION     DASUGB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009806"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1195
                     /locus_tag="VFV87_06195"
     CDS             107..1195
                     /locus_tag="VFV87_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5103380.1"
                     /translation="MQIFVFEFITGGGLLSLPGAPKPCGSLLAEGRAMAAAVSADFLA
                     LQDVELLTTRDSRLSPLHPDAADVTIVRSVADERTAFDALAKTADWTLVIAPETAGAL
                     LQRAEQALSAGGRLLSPLPDLVRLASDKQQTADWLLAHGIRAPRGCVVDRTNPPPVDF
                     PFPAVLKPNGGCGSQDIRLVSNVGRVSNPSAAGLVSNPSLRLRLEPLIPGLAASVSIL
                     CGPAGHVALPACEQRLSADGRFRYLGGLTPLPAALDRRARQLALAAIHTLPAPIGYIG
                     VDLVLGDEARGAADFVIEINARLTTSYVGLRAACRENLAAAMLAIATGSLATLSFHPQ
                     PLEFDADGMIHATAANLQAPTELARWQP"
     gene            1205..2218
                     /locus_tag="VFV87_06200"
     CDS             1205..2218
                     /locus_tag="VFV87_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5103381.1"
                     /translation="MNWLALDIGGANLKAADGQGHAQSYAFALWRDPHGLAQHIRTAI
                     SEAPQTTHLAVTMTGELADCFESKADGVRFILQAVHTASDNRHTRVYLVDGRLVSPQV
                     ALTMPHLVAAANWHVLARYAGRHAPAGTSLVIDCGSTTCDLIPLADGKPVAQGATDTQ
                     RLLAGELVYTGVERSPVCGVADFGLYRGQSCPIVHELFATMRDVYLLLDRLEPNPADT
                     HTADNRPATKAAARARLGRMIAADSEEFNHRDAVTLAQSIADAQVARLRAAIAKVAAR
                     LPAPPVKIIYSGHGEFLAQAAVESLGLNVPAVSLGRELGPTISRSATAHALATIAREA
                     TAT"
     gene            2579..3178
                     /locus_tag="VFV87_06205"
     CDS             2579..3178
                     /locus_tag="VFV87_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103382.1"
                     /translation="MSNRPQPCGLRIVKVGGSLLDWPQLAEQLSRWLESQPRQPSVLI
                     CGGGDFTDAVRRAQAIHRFDDETAHWMCVRALSVTAQLLARLLPGAKLAGSLDDVVAL
                     ARGGAEQIVFDAGAFLRAEEPQVVGLPLPHDWTATTDSIAARIAELTGASELILLKSS
                     DPPPHRSLADLAVAGYVDEHFPLAAAELPAIRLVNVRRE"
     gene            3229..3954
                     /locus_tag="VFV87_06210"
     CDS             3229..3954
                     /locus_tag="VFV87_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HEX5103383.1"
                     /translation="MATDRPITLLSQIRRWVRRAAVAAAGLALLYAGFLLLGFVPVNR
                     GYSAPPRDNCVRIYVRSNEVHTDLVLPVVCDEVAADWRLLFPLRDFWGNVKEPEFVAF
                     GWGNRDFYVNTPTWAEFRLSTACRALFWPSETVLHVEYLDGVTPGDQMRPILLTREQY
                     RELADFVAASVGETNASGQALTVTEFSYGRSDRFYSGAGRYHCFNTCNQWTGRGLKRA
                     GVPTGLWTPLKPQVLYWLPAVED"
BASE COUNT          652 a         1429 c         1206 g          728 t
ORIGIN      
        1 ggaacggatg tcaatgagca aggcgaagcg gcttcgccac cgcttggacc ccgaacattg
       61 tactccatcg acaatctgcc ggacgccgat tccagcggaa tttcccgtgc aaatcttcgt
      121 ttttgagttc atcaccggag gcggactttt gagcctgccc ggggctccaa agccctgcgg
      181 ctcgctgctg gcggagggcc gggcgatggc ggccgcggtc agcgccgatt tcctggctct
      241 gcaggatgta gagcttctga ctacccgcga ttcccgactc tcgccgctgc atcccgacgc
      301 cgcagacgtg acgatcgttc gttcggtggc tgacgagcgg acggcattcg acgctctggc
      361 aaaaacggct gattggacgc tcgtcatcgc tcccgaaacg gccggcgcac tcttacagcg
      421 agccgagcaa gccttgtccg ccggcgggcg cctgctctcg ccgctgccgg accttgttcg
      481 cctggccagc gacaagcagc aaacggccga ttggcttctc gcccacggca ttcgggcccc
      541 tcgcggctgc gtcgtcgatc gcacgaatcc acctcccgta gatttcccgt ttcccgccgt
      601 cctcaagccg aatggcggct gcggctcgca ggacattcgt ctcgtttcca acgtaggacg
      661 ggtttccaac ccgtctgccg caggactcgt ttccaacccg tccctccgcc tccgactcga
      721 accactcatc cccggcctcg cggccagcgt cagcatcctc tgcggccccg ccggccacgt
      781 ggccctcccc gcctgcgagc agcgcctctc tgcggacggc cgcttcagat atctcggcgg
      841 cctcacgccg ctgcccgcgg cgctcgatcg ccgcgcccgg caacttgcac tagccgccat
      901 tcacaccctc cccgcgccaa ttggctacat cggtgtcgat ctggttctgg gtgacgaagc
      961 gcgcggcgcc gccgacttcg ttattgaaat caatgcccgc ctgaccacct cgtacgtcgg
     1021 cctgcgcgcc gcctgccgcg aaaacctggc cgccgccatg cttgccattg ccacgggaag
     1081 cctcgccact ttgtcgttcc acccccagcc gctagaattc gatgccgacg gcatgatcca
     1141 cgctacagcc gccaaccttc aggcccccac cgagcttgct cggtggcagc catgattttg
     1201 cactatgaac tggctggctc tcgacatcgg cggcgcgaac ctgaaggcgg ccgacggcca
     1261 aggccacgcc cagtcgtacg ctttcgcctt gtggcgcgac ccgcacggct tggcccagca
     1321 catccgcacc gctatctccg aggccccgca aacgacccac ctcgcggtca cgatgacggg
     1381 cgagctggcc gattgctttg agtccaaagc cgacggcgtg cgtttcattc tccaggccgt
     1441 ccacacggct tccgacaatc ggcatacgcg cgtctatctg gtcgatggcc ggctggtatc
     1501 gccgcaggtc gccctcacca tgccgcatct tgtggccgcg gcgaactggc acgtattggc
     1561 ccgctatgct ggccgccacg ctcctgcggg aacgtcgctc gtcatcgact gcggttctac
     1621 gacctgcgac ttgattccac tggcagacgg caagcccgtc gcccagggcg ccaccgatac
     1681 gcagcgcctg ctggcaggcg agctcgtcta tacgggcgtc gagcgcagcc cggtctgcgg
     1741 cgtggccgat ttcggcctct accgcggcca gagctgcccg atcgtccacg agctgttcgc
     1801 caccatgcgc gacgtctatc tcctgctcga caggctggag ccgaacccgg ctgacaccca
     1861 taccgccgac aaccgcccag ccaccaaagc cgccgcccgg gcacgcctcg gccgaatgat
     1921 cgcggccgac agcgaggagt tcaaccaccg cgacgccgtg accctcgccc aaagcatcgc
     1981 cgacgcccag gtcgcgcgcc tcagggcggc gatcgccaag gtcgccgccc ggctgcccgc
     2041 cccgccggtc aagatcatct actctggcca tggcgagttc ctcgcccagg ccgctgtgga
     2101 gtcgctgggc ctcaacgtac cagccgtctc tctaggccgc gagctcggtc cgacgatctc
     2161 acgctccgcc accgcgcacg ccctggcgac catcgcccgg gaagcaacgg cgacctgact
     2221 cgcgcaacat ttgagttgcg cgcaactttt cagaattctt tcacgccacg cactcccgcg
     2281 caacatcatc gtaaccttca tcacgaaaac aacttgcgcc gcgcaacacc ccccgcatga
     2341 ggatgttgcg ctgttgcgca ttgtcaagag ctttggagac ccacgctcga cccgtcattt
     2401 cgccgcggtg ctcaccgcgt atcggcgatg agggtcagac cgacgaaccc ggaaccttct
     2461 caaccaggat gtcaatgagc gcgcgaagcg accgtgcggg acggaagccc gatcaaacgt
     2521 ttcgcggccc ggatttgcgc accgggcagc atccccaatt atatagttcc gttcactaat
     2581 gtcaaataga ccacaaccct gcgggctgcg gatcgtcaaa gtcggcggga gcctgctcga
     2641 ctggccgcag cttgcggagc agctaagccg ctggctggaa tcacagccgc ggcaaccttc
     2701 ggtgctgatc tgcggcggcg gcgattttac cgatgcggtc cggcgggcgc aggccataca
     2761 tcggttcgac gacgagactg cccattggat gtgcgtccgg gcactgtcgg tgacggcgca
     2821 gcttctggcc cgccttttgc ctggggcgaa gcttgctggt tcgcttgatg acgtggtcgc
     2881 gctggcccgg ggcggggcag aacagatcgt cttcgacgcc ggcgcgtttc tgcgcgcgga
     2941 ggaaccgcaa gtcgtcggcc tcccgctgcc gcacgactgg acggcaacga ccgactcgat
     3001 cgccgcccgc atcgcggaat tgaccggcgc cagcgagctg atcctgctca aatcctccga
     3061 tccgccgccg caccgctccc tggccgacct ggccgttgcg ggctacgtgg atgagcactt
     3121 cccgctcgcc gctgccgaac tgccagcaat cagattggtg aacgtgcggc gggaataatc
     3181 tccatccacg cagcgtcaat gcatcggact gcaactcccg tggtgacgat ggcaaccgat
     3241 cgaccgataa cgctcttgag ccaaatccgc cgctgggtcc gccgggcggc tgtcgcggct
     3301 gctgggctcg cgttgttgta cgctggcttc ctgctgctgg gttttgtgcc ggtgaatcgc
     3361 ggctattccg cgcctccgcg ggacaactgc gtccgcatct atgtgcggag caacgaggta
     3421 cataccgacc tcgtgctgcc ggtcgtctgt gacgaagtcg cggcggactg gcggctgctg
     3481 tttccgcttc gcgacttttg gggcaacgtg aaagagcccg aatttgtcgc tttcggctgg
     3541 ggaaaccgcg atttctacgt caatacgccc acctgggccg aattccgtct ttcgacggcc
     3601 tgccgagcgc tcttctggcc gagcgaaacc gtgctgcacg tcgagtatct cgacggtgtg
     3661 acgcccggcg accagatgcg gcccatcctg ctgacgcgcg agcagtacag ggagttagcc
     3721 gacttcgtgg ccgcctcggt gggcgaaacg aatgcctcgg ggcaggccct cacggtcacc
     3781 gagttcagct acggccgctc tgaccggttc tattcgggtg cgggccgcta tcactgcttc
     3841 aatacctgta accagtggac ggggcgtggg ctcaagcggg ccggcgtgcc gaccggcctc
     3901 tggacgcctc tcaagccgca ggtgctctat tggcttcccg cggtggagga ctaacgcagg
     3961 cttgatgcca gcgcccgccg gcgaccggct tcgggggcgg gcgacggtgc ggacg
//
LOCUS       DASUGB010000324         8393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_175390, whole genome shotgun sequence.
ACCESSION   DASUGB010000324 DASUGB010000000
VERSION     DASUGB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175390"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3237)
                     /locus_tag="VFV87_06215"
     CDS             complement(<1..3237)
                     /locus_tag="VFV87_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5103384.1"
                     /translation="MASFFIDRPIFAAVISVIITLAGGIAVFVLSVAQYPEITPPTVE
                     VSCTYPGASAKVVADTVAAPIEQQVIGVENALYMSSQSTNDGGYKLTVTFAVGTNLDM
                     AQVLVQNRVNLALPSLPSEVKATGVSVKKKSPNILLVVNLISPNGSYDQLYLSNYATI
                     RVRDELAQIKGVGDVAYLGQLDYSMRAWIDPERMAARDLSASDLVNALKEQNVQVAAG
                     ALGRPPVPTGQAFQYTLSTLGRLTDPEEFGNIVVKTGADGQLIRLRDLVTDERTDDSG
                     NKFGGIQLGAKVEDTSCRLDGQPAIGLAIYQQPGSNALKTADAIRARMDELALSFPPD
                     LKYKIVYDTTPFIDESIHEVFKALRDAIILVALVVLAFLQSWRATIIPLIAVPVAIVG
                     TFAVMLGMGFSLNNLSLFGLVLAIGIVVDDAIVVVEAVEHHLEHGLSPREAARKAMGE
                     VTGPIIAISLVLMCVFIPCAFITGITGQFFRQFALTIAVSTFFSAVNSLTLSPALCGL
                     LLKAKHEQRDPLSWLLNLALGWFFKLFNLGFQCASGGYSQFVRMTLRVSLIVLLIYVG
                     LLGLTAFSFTRVPVGFIPSQDKGYLLVDVRLPDSASLERTKAVMAQVESIARGAAKPA
                     HGGDEHATAEHAAADHAAGEHAGGGVPGVAHTITISGQSIVQNAIGSNYGTVYVVLDE
                     FHHRHGAERGADAIAAKLRAAFYRDVQEAQVSVFGAPAVDGLGNAGGFKVMVRDTGAL
                     GLDALQEAADGLAAAGNEQPGIVGMFSAFRARTPQMYVDIDRERCKSMGVPLNEVFLT
                     LQLYLGGYYTNDFNQFGRTWQVNLQGDPRFRLTPEQVRQLKVRNSSGEMVPLGSVASV
                     SPTGGPALVIRYDGVTAAAINGASAPGVSSGTVIKTVEEVAARELPLGMNVRWTELTY
                     LQIQAGNTAIYVFIAAVVLVFLVLAAQYESLTLPLGVILVVPMCLLSSVIGVWLAGMD
                     INIFVQIGFVVLVGLAAKNAILIVEFAKEKSAQGATPADATVEACRLRLRPIIMTSLA
                     FILGVVPLALAVGAGAEMRSTLGIAVFSGMLGVTIFGIFLTPVFYYVIV"
     gene            complement(3243..4589)
                     /locus_tag="VFV87_06220"
     CDS             complement(3243..4589)
                     /locus_tag="VFV87_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5103385.1"
                     /translation="MAAPQPYRSTCLAASVLAAMAGLLLVSSGCDSSAPAASELPAPK
                     VTTMAVVLQETIDADEYQGQTEASETVEVRARVFGYLKTIDFNDGDFVKGPVLGPGGE
                     VQEEGQILFTIEPDEYDAIHKQSLARIDLNQANLTLAKAKHARNATLVKSGAVTQEEF
                     EESAAAVASAEAAITAAKADANRTAVDLKYTVIRAPIDGRIDRAMVSVGNLLTGGMSS
                     GTLLTKIVKESPMYVYFDVDERSLLRYMRRRAETRQTAPGSLRDLGIPIYLQLADETE
                     FSHEGTLDFVETEVSRTTGTARIRGVFANTDRTLASGLFVRVQIPVSEPYQALLLPEA
                     ALATDQNIKYVYVVAGGKAERRNVELGGQRGDMRIITSGLKEGDQVIVKGLQRVKPGQ
                     QVEAEQAAAPEMPPTTVRKPVTPPTSPEPPAKEEIDPGPVETAPATSTPRTRAQER"
     gene            complement(4701..5948)
                     /locus_tag="VFV87_06225"
     CDS             complement(4701..5948)
                     /locus_tag="VFV87_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103386.1"
                     /translation="MPAGRPGGSTALLPGLGNRPDIGNRPSQLPNRTPQQRRDDLQNR
                     LASRDQIADRDNIREDWHDRYGDFYDRHDGWHHGCWDGNEGWWGHMWSEHTALMAWGT
                     TMWGLNRLAYGFGYWGYDNPYYSDSYSYPVDYSQPLPEEVAPLEQPAGASTPGMADFD
                     AARAAFLKGDYATALASTDKALAALPGDPIIHEFRALVLFALGKYPEAAAGLYSVLSV
                     GPGWDWTTLISLYPSMDVYTKQLRALEAYVKANPDAADARFVLAYHYLTQGNQDAAAS
                     QYKKLYEKTPQDTVIKELLLLAGGEEAVAGATTPAAPATAAVRINVAELVGRWSASNE
                     SGAKFVLDLTQDGNFTWTYSQDGKSQAVKGVYALDGDTLAMEPETGGTMLAQVTRPQG
                     GSFGFALLGAPPGDPGLKFAKAQ"
     gene            6004..6381
                     /locus_tag="VFV87_06230"
     CDS             6004..6381
                     /locus_tag="VFV87_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103387.1"
                     /translation="MLGSVPTPGVPAPGRVLGSVLMPGREPGVRPGVAGRVPAVGNWT
                     EPGRLPGVRPGEGRVAGRAPVDGSEGRAAGREATLGGRLIDGERPIEGEGRAIPPPPM
                     RAPPPPPNPPPPPPRPPPPPPRP"
     gene            complement(6512..7438)
                     /locus_tag="VFV87_06235"
     CDS             complement(6512..7438)
                     /locus_tag="VFV87_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5103388.1"
                     /translation="MLFCRWLLAASLIVMAATPTWAQQPARTAKAENAISADEQAIRK
                     AVTAFVELYNAHNADGLTALFAPDARMTFRDGAEVNGREEIKQSFVQAFTDTPKCAVS
                     VVVDSFRFLTPDVAVEEGSTSLFPDGETLVAKSRYTVVHLKKDGKWLMQSVRIVEEES
                     LSAYGELQPLEWLIGDWIDEGRTENVETTFRWDDNKSFLLEEFQVVRDGEVVMKGTQR
                     IGWDAQARQIRSWTFDSAGGFGEATWTPVEDRWVVKAKAVTADGTSASATRTLTRATP
                     DRVIWTATDRLAGDEQLPDLAVTMVRKAPKPE"
     gene            7682..>8393
                     /locus_tag="VFV87_06240"
     CDS             7682..>8393
                     /locus_tag="VFV87_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5103389.1"
                     /translation="MSASSPANQVPNRPSRLRRLMPIVQWLPTYQRAWLRWDLVAGIT
                     LAAYAIPVSLAYASLAGLPPQMGLYCYLVGGLGYVLLGSSRHLAIGPTSAISLLLGVS
                     LLEYSAGDQERQAGLASLTALFMAGVFLLAWLLRLSVLVNFISESILTGFKAGAALVI
                     AATQLPKLLGVTGGGESFFERIWLLVQQANATNLITLTIGLAALALLLAGDKYLPGRP
                     VALVVVLLSLLAVWLGGLD"
BASE COUNT         1393 a         2671 c         2812 g         1517 t
ORIGIN      
        1 gacgatgacg tagtagaaga cgggcgtgag gaagatgccg aagatggtga cgccgagcat
       61 gccgctgaag acggcgatgc cgagggtgct gcgcatctcc gcgccggccc cgacggcgag
      121 ggccagcggc acgacgccga ggatgaaggc cagcgaggtc atgatgatcg gccgcagccg
      181 caggcggcag gcttcgacgg tggcgtccgc gggagtcgcg ccctgggccg acttttcctt
      241 cgcgaactcg acgatcagaa tcgcgttttt ggcggccagg ccgacgagca ccacgaagcc
      301 gatctgcacg aagatgttga tgtccatgcc ggccagccac acgccgatca ccgacgacag
      361 caggcacatc ggcacgacga gaatcacccc caggggcagt gtcaggctct cgtactgcgc
      421 ggcgaggacc aggaacacga ggacgaccgc cgcgatgaag acgtaaatcg ccgtgttgcc
      481 ggcttggatt tgcagatacg tcagctcggt ccaccgcacg ttcatgccca ggggcagttc
      541 gcgggcggcg acctcctcga ccgtcttgat cacggtgccg gagctgacgc cgggggccga
      601 ggcgccgttg atggcggcgg cggtgacgcc gtcgtaacga atgaccagag ccgggccgcc
      661 ggtggggctg acgctggcca cgctgccgag cggaaccatt tcgccgctgg agttgcggac
      721 cttgagctgc cgtacctgct cgggagtcag gcggaaccgc ggatcgccct gcaaattcac
      781 ctgccaggtg cggccgaact ggttgaagtc gttggtgtag tagccgccga gatagagctg
      841 cagcgtcaaa aacacctcgt tcagcggcac gcccatgctc ttgcagcgct cgcggtcgat
      901 atcgacgtac atctgcggcg ttcgcgcgcg gaaggcgctg aacataccga cgattcccgg
      961 ctgctcgttg cccgcggctg ccagcccgtc ggcggcttct tgcagggcgt cgagccccag
     1021 agcgcccgta tcgcggacca tcaccttgaa gccgccggcg ttgcccaggc catcgaccgc
     1081 cggcgcgccg aagacggaga cctgggcctc ttgaacgtcg cggtagaacg cggcgcggag
     1141 cttcgccgcg atggcgtcgg cgccccgctc cgcgccgtgc cgatggtgga actcgtcgag
     1201 cacgacatag acggttccgt agttcgaacc gatcgcgttc tgcacgatcg actggccgga
     1261 aatcgtgatc gtgtgggcga ctccgggaac gccgccgccg gcatgctcgc ccgccgcgtg
     1321 atcggccgcc gcatgttcgg ccgtggcgtg ctcgtcgccg ccgtgggctg gcttggccgc
     1381 gccgcgggcg atcgattcca cctgggccat caccgccttg gtccgctcca gcgaagcgga
     1441 gtccggcagc cgcacatcga ccagcagata tcctttgtcc tgcgacggaa tgaaaccgac
     1501 gggcacgcgg gtgaagctga acgccgtcaa gccgagcagg ccgacataaa tcagcagcac
     1561 gatcaggctc acccgcagcg tcatccgcac aaactgggag tatccgcccg acgcacactg
     1621 gaaacccagg ttgaacagct tgaagaacca gccgagcgcc aggttgagta gccacgagag
     1681 gggatcgcgc tgctcgtgct tcgctttgag caacaagccg cacaaagccg ggctgagcgt
     1741 gagcgagttc accgcggaga agaacgtcga gacggcgatc gtcagggcga actgccggaa
     1801 gaactgcccc gtgatgccgg tgatgaacgc gcacgggatg aacacgcaca tcagtaccag
     1861 tgaaatggcg atgatcggcc cggtcacttc acccatcgcc ttgcgggcgg cttctcttgg
     1921 ggagaggccg tgctccaggt gatgttcgac cgcttcgacg acgacgatcg cgtcatcgac
     1981 aacaatgccg atcgccagca ccaggccgaa cagcgagagg ttattgaggc tgaagcccat
     2041 gccgagcatg acggcgaagg tgccgacgat cgcgaccggc acggcgatca gcggaatgat
     2101 cgtggcccgc cacgattgca ggaaggcgag caccacgagg gccacgagaa tgatcgcgtc
     2161 gcgaagggcc ttgaacacct cgtggatcga ttcgtcgatg aacggcgtcg tgtcatagac
     2221 gattttgtat ttgagatccg gcggaaaact gagcgccagc tcgtccatcc gcgcgcggat
     2281 ggcatcggcc gttttcaggg cgttcgatcc gggctgctgg tagatggcca ggccgatggc
     2341 gggctggccg tcgaggcggc acgaggtgtc ttcgaccttg gccccaagct gaatgccgcc
     2401 gaatttgttg ccgctgtcgt cggtccgctc gtcggtcacc agatcgcgga ggcggatgag
     2461 ctgcccgtcg gctccggtct tgacgacgat gttgccgaac tcctcgggat cggtcaggcg
     2521 gccgagcgtg ctgagcgtgt attgaaaggc ctggccggtc gggaccggcg gccggccgag
     2581 cgcgccggcg gcgacttgca cgttttgctc tttaagggca ttgaccagat cgctggccga
     2641 caggtcgcgg gccgccatcc gctccggatc gatccaggcc cgcatgctgt aatcgagctg
     2701 gcccaggtac gccacatcgc cgaccccttt gatctgggcc aactcgtcgc gcacgcggat
     2761 cgtggcatag ttgctgagat agagctggtc gtagctgccg ttcggcgaaa tcaggttgac
     2821 gacgagcagg atgttcggcg atttcttctt cacgctgacg cccgtcgctt tcacttcact
     2881 cggcaacgac ggcagggcga ggttgacgcg gttctgcacg agcacctggg ccatatccag
     2941 gttcgtgccg acggcaaacg tcacggtcag cttgtagccg ccgtcgttgg tcgattgcga
     3001 gctcatgtac agagcgttct cgacgccgat cacctgctgc tcgatcggcg cagcgacggt
     3061 gtcggcgacg accttcgcgc tggcgccggg atacgtacag ctcacctcga ccgtgggggg
     3121 cgtgatctcg gggtactgcg ccaccgacag cacgaacacg gcgatgccgc ccgccagcgt
     3181 gatgatgacc gagatgactg ccgcgaagat cgggcggtcg atgaagaaac tggccacttc
     3241 gtctagcgct cctgcgcgcg agtgcgggga gtggaagtcg cgggggccgt ttccaccgga
     3301 ccggggtcga tttcctcttt cgcgggaggc tccggggacg taggcggcgt cacgggcttg
     3361 cggacggttg tcggcggcat ttccggagcg gcggcctgtt cggcttcgac ctgctggccc
     3421 ggcttgacgc gctgcagccc tttgacgatg acctgatcgc cttccttcag gccggaggtg
     3481 atgatccgca tgtcgccccg ctggccgccg agttcgacgt tgcggcgctc cgctttgccg
     3541 ccggcgacca catagacgta cttgatgttc tggtcggtcg ccagggccgc ttcgggaagc
     3601 aagagtgcct ggtagggctc gctgacggga atctgcacgc ggacaaacag gccgctggcc
     3661 agggtgcgat ctgtgttggc aaagaccccg cgaatccggg ccgtgccggt ggtgcggctg
     3721 acctcggttt cgacgaagtc gagcgtccct tcgtgcgaga actcggtttc gtcggcgagc
     3781 tgcaggtaaa tcggaatccc caggtcgcgc aggctgccgg gagcggtctg gcgcgtttcc
     3841 gcccgacggc gcatgtatcg caagagcgac cgctcatcga cgtcgaaata gacatacatt
     3901 gggctttcct tgacgatctt cgtgagcaaa gttcccgacg acatcccacc cgtgagcagg
     3961 ttgcccacgg agaccatcgc gcggtcgatc cggccgtcga tcggcgcgcg aatcacggtg
     4021 tatttcagat cgactgctgt gcggttggcg tcggccttgg cggcagtaat cgccgcttcc
     4081 gccgaggcaa ccgcagcggc cgactcttcg aactcctcct gcgtaacggc gccggatttc
     4141 acgagcgtgg cgttgcgggc gtgcttggcc ttggcgagcg tgaggttggc ctggttcaga
     4201 tcgatccggg cgagcgactg cttgtgaatc gcgtcgtatt cgtcgggctc gatggtaaac
     4261 aggatctggc cctcttcctg cacctcgccg cccgggccga gcacgggtcc tttgacaaaa
     4321 tcgccgtcgt tgaagtcgat cgtcttcagg tagccgaaga cccgcgcccg cacttcgacg
     4381 gtctcggagg cttcagtctg cccctgatac tcgtccgcgt cgatcgtctc ttgcaagacg
     4441 accgccatcg tggtgacctt gggcgccggc aattcgctgg cggcgggtgc cgagctgtcg
     4501 cagccggaac tgacgagcag cagccctgcc atcgccgcca ggaccgatgc ggcgaggcaa
     4561 gtcgatcgat aaggttgagg ggcagccatg agattctcgt gagcacgagt cagtgcggag
     4621 cgaggcagtg cgaagcagga tggattctac tgctccgccg cgtggttgtc gccggttgag
     4681 tggctactgc gctggaatac ctactgcgct ttggcgaatt tcagtcccgg atcgcccgga
     4741 ggagctccca gcagggcaaa gccgaagctc ccgccttgtg gccgggtgac ctgggcgagc
     4801 atcgtgccgc ccgtctccgg ctccatcgcc agcgtgtcgc catccagggc atagacgccc
     4861 ttcaccgctt gggatttgcc gtcctgcgag taggtccagg tgaagttgcc gtcctgcgtc
     4921 aggtcgagca caaacttggc tccgctttcg ttgctggcgc tccagcggcc gaccagctcg
     4981 gcgacgttga tcctgacggc ggctgtcgcg ggcgcggccg gcgtcgtcgc cccagcgacc
     5041 gcctcctcac cccccgccag cagcagcaac tccttgatga ccgtgtcctg cggcgtcttc
     5101 tcatagagct tcttatactg agacgctgcc gcgtcctgat tgccttgcgt gagatagtga
     5161 tacgcgagga caaaacgtgc gtctgccgcg tcgggattcg ccttcacgta agcctccagg
     5221 gcccgcaact gcttcgtata aacatccatg ctgggataga ggctgatgag cgtcgtccag
     5281 tcccagccgg gaccgacgga gagcacgcta tagagaccag ccgcggcctc cggatacttt
     5341 cccagcgcga acagcaccag ggcgcggaat tcgtgaatga ttggatcgcc cggcaacgcg
     5401 gcgagggcct tgtcggtcga ggccaacgct gtcgcgtaat cacccttcaa aaacgcggcg
     5461 cgagccgcgt cgaaatcggc cattcccggc gtcgacgcgc cggcgggctg ctcgagcggt
     5521 gcgacttcct ccggcagcgg ctgcgagtaa tcgacgggat aagagtagga gtccgagtaa
     5581 taggggttgt cgtagcccca gtagccgaag ccatacgcca ggcggttgag gccccacatt
     5641 gtggttcccc acgccatcag ggccgtgtgc tcgctccaca tgtgccccca ccagccctca
     5701 ttgccgtccc agcagccgtg atgccagcca tcgtggcggt cgtagaagtc gccatagcga
     5761 tcgtgccagt cctcccgaat gttgtcgcgg tcggcgattt gatcgcgcga agcgaggcgg
     5821 ttctgcaggt cgtcgcggcg ctgctgcggt gtgcgattgg gaagttggct cggccgattg
     5881 ccgatgtccg ggcggttgcc cagtccgggc aagagcgccg tgctgccgcc gggtcggccg
     5941 gcgggcaatt gactgatccc gggggcgccg ggacgggagc ctggcgtacc gacgccgggc
     6001 cgcgtgctgg gcagcgttcc cacaccggga gtgcccgcgc cgggccgcgt gctgggcagc
     6061 gtgctgatgc cggggcgcga gccgggcgtc aggcccggcg tggctggacg cgtgccggcc
     6121 gtcgggaatt ggacggaacc gggccggctg ccgggggtga ggccgggcga aggccgcgtc
     6181 gcgggccgag cgcccgtcga tggcagcgag gggcgagcag ccggccgtga agcgacgctc
     6241 ggcgggcggc tcattgacgg cgagcggccc atcgaaggcg agggccgcgc catcccaccg
     6301 cctcccatgc gagcgccgcc gcctccgcca aatccgccac cccctccacc gcggccgccg
     6361 cctccgccgc cgcggccgta agctgttatc gctgggccca aggccatcat tccaacgagc
     6421 agcacgctcg ttacctgccg catgttccgt ttcatggctg tatcccggtt tgtgatcgtt
     6481 atggattggt gagtgtgaat agcgcgccga ttcattccgg cttgggggcc ttgcggacca
     6541 tcgttacggc cagatcgggc agttgctcgt cgccagccag ccggtcggtt gccgtccaga
     6601 tcacgcggtc gggcgtggcc cgggtcaacg tgcgcgtcgc ggaagcagac gtcccgtccg
     6661 cagttacggc tttggccttc acgacccagc ggtcttcgac cggcgtccag gtcgcctcgc
     6721 caaagccgcc ggccgagtcg aaggtccacg agcgaatctg cctggcctgt gcatcccagc
     6781 cgatgcgctg ggtgcctttc atgacgacct cgccatcgcg gacgacctgg aactcttcca
     6841 gcagaaagct cttgttgtcg tcccagcgga acgtcgtttc gacgttttcc gtgcgccctt
     6901 cgtcgatcca atcgccaatc agccattcga gcggctgcaa ctcgccataa gccgagagcg
     6961 attcctcctc cacgattcgc accgactgca tcagccactt gccgtccttt ttcagatgca
     7021 cgaccgtgta ccggctcttg gcgacgagcg tttcgccgtc gggaaagaga ctggtcgagc
     7081 cttcttccac ggccacatcg ggcgtgagga agcggaacga atcgacgacg acgctgaccg
     7141 cgcacttggg cgtatccgta aaggcctgta cgaacgattg tttgatttcc tcccgtccat
     7201 tgacctcggc gccgtcacgg aaggtcatcc gggcgtccgg tgcgaagagc gccgtgaggc
     7261 cgtcggcatt gtgcgcgttg tagagctcga caaaggccgt gaccgccttg cggatggcct
     7321 gttcgtcggc cgagatggcg ttctcggcct ttgctgtccg ggcgggctgc tgcgcccagg
     7381 tcggagtcgc cgccatcacg atgagcgatg cggccaataa ccagcggcaa aaaagcatgg
     7441 atactctccc aaagaagcgg cgaaggtgcg ccgtaacgtg atcgcttgat agaatgcgtg
     7501 ccgaacctgc ccaagcgccc aagccgcgcc tggaaatgag gaagaggtcc atcattagac
     7561 tacgcggaag ccacagaatc aattgaccat cgcggttttg cggatcgttc accggcgcgg
     7621 cataacccgt atgctggtac gtgttctttg gccgactggc cgtgcgtgtc gtgcttctcc
     7681 catgagtgca agcagtccag cgaatcaggt ccctaaccgg ccatcacggc tgcggcggct
     7741 gatgccgatc gtgcagtggc tgccgacgta tcagcgggcg tggctccgct gggatctggt
     7801 tgcaggaatc acgctggcgg cgtacgccat tcctgtctcg ctggcgtacg cctcgctggc
     7861 cggtctgccg ccgcaaatgg gactgtattg ctacctcgtg gggggcctcg gctatgtgct
     7921 cctgggctca tcgcggcatc tggcgatcgg ccccacctcg gcgatttcgc tgctcctggg
     7981 cgtgtcgctg ctcgaatact cggccggcga ccaagagcgc caagcggggc tggcctccct
     8041 gacggccttg ttcatggcgg gcgtgttctt gctggcgtgg ctactgcggc tcagcgtcct
     8101 ggtgaacttc atttccgaga gcatcctcac gggattcaaa gcgggcgcgg cgctggtgat
     8161 cgccgccacg caactgccca aattgctcgg cgtgaccggg ggcggcgaga gcttctttga
     8221 gcgcatctgg ctcctcgtgc agcaggccaa cgcgacgaac ctgatcacgt tgacgatcgg
     8281 actggcggcg ctggcgctgc tgcttgcggg cgacaaatac ttgccgggcc ggccggtggc
     8341 cctcgtcgtg gtgctgctct ccctgctggc cgtgtggctc ggcgggctcg atc
//
LOCUS       DASUGB010000325         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1704024, whole genome shotgun sequence.
ACCESSION   DASUGB010000325 DASUGB010000000
VERSION     DASUGB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704024"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFV87_06245"
     CDS             <1..402
                     /locus_tag="VFV87_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5103390.1"
                     /translation="ADDSVLAQMSPPDMKLPIQYALTWPQRRDSPARKLDLTAALRLD
                     FEPPDEDRFPALALGRQVAAAGGTAGAVLNAANEAAVASFLAGELAFTEIVPACRSVL
                     ECHDFNADPALDELLALDAWARQEISRWICA"
     gene            456..>2546
                     /locus_tag="VFV87_06250"
     CDS             456..>2546
                     /locus_tag="VFV87_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5103391.1"
                     /translation="MLDCWGSLGEQLLLGASTPEPSTFAWLLSKTWIILSIAVGLGFV
                     IFVHELGHFLVAKACGVQCDKFYIGFDIPMPRIFGWQMPSRLARFRWGETEYGVGILP
                     LGGYVKMLGQDDDPRNAEREAERTRVLKEASAAAGLSPGQTVEKEYQLDPRSYPAKPV
                     PARMAIISAGVIMNLIFAVIFGAIAYRYGVPEMPATIGGTMPGSPAWAADIQPGSKVL
                     QFGKPGRKYEYLRWEDLMREVVLNGSDRSLPMLVRNSDGQEVWHEIRPSDRLKAIKAL
                     NNRPTIGVAPQKTRQVRVRSEEPVYLAATVAPALEEKDVIVAVAEKRLEADWQLTELL
                     ANRPSGPLAVTVSRIKQGPDGKELKPQVKEEFDAVIQPRAMRELGLVMKLGPVVAVRE
                     NSPAAVAGIQVDDEIETLDGQDVGDPLTLGQRMLPGDNPNRTFEVGIRRKDRGGKWIT
                     KKLVVHPELPQQYQNLFPNHINGGPAAVESIGLAVDVLNVVSAVTPGGPADIAGLLPG
                     DVVTQYQLVAASQPAAEQETRFLKPVLDVDKPFDPVVLDQLNNNWISLLARMQLLDPQ
                     TRVKLTWDRNGKSTSEVLQPIDSTKWWDESRNLSFYTQKVTHRAENWPDAFSLGLRET
                     SERLKEVLRVLSSLVTGRLSATNLSGPAGIFVAAGSFASEGIPSLLIFLTLLSANLAV
                     LNFLPIPALDGGHML"
BASE COUNT          461 a          803 c          838 g          444 t
ORIGIN      
        1 gccgatgact cggtgctggc tcaaatgagc ccgcccgaca tgaagttgcc gatccagtac
       61 gccctgacct ggccccagcg gcgcgacagc ccggcccgca agctcgattt gaccgcggct
      121 ctgcggctcg atttcgagcc gccagacgag gatcgttttc cggccctggc tctgggtcgc
      181 caagtggcgg cagccggcgg cacggcgggg gccgtgctca acgcggccaa cgaggccgcg
      241 gtcgccagct tcctggccgg cgaattggcc tttacggaga tcgtcccggc ctgccggtcg
      301 gtgctcgaat gtcatgactt taacgccgac ccggcgcttg acgagctgct cgcgctcgac
      361 gcctgggccc ggcaggagat ttcgcggtgg atatgtgcct aacctcggcg gcctgcgatt
      421 tgtgccccgc cgtccattca cttgtattgc tgaacttgct ggactgttgg gggtcgctcg
      481 gcgagcagct gctgctgggc gcgagcactc cggagccgag cactttcgcc tggctgctat
      541 ccaagacctg gatcatcctg agcatcgctg tcggcctggg ttttgtgatc ttcgtccacg
      601 agctggggca cttcctcgtc gccaaggcgt gcggcgtgca gtgcgacaag ttctatattg
      661 ggttcgatat cccgatgccc agaatcttcg gctggcagat gcccagccgg ctggctcgct
      721 tccgctgggg ggaaaccgaa tacggagtcg gcatcctgcc gctgggcggc tacgtaaaga
      781 tgctggggca ggacgacgac ccaagaaacg ccgagcgcga agcggagcga acgcgagtcc
      841 tgaaagaagc gtcggccgcg gcagggctca gccccgggca gaccgtcgag aaggagtatc
      901 agctcgaccc gcgcagctat ccggccaagc ccgttcccgc ccgcatggcg atcatttcgg
      961 ccggcgtgat catgaacctg atcttcgccg tgatctttgg ggccattgcc tatcgctacg
     1021 gcgtgccgga aatgcccgcc acgatcggcg gcacgatgcc cggcagcccc gcctgggcgg
     1081 ccgacatcca gcccggcagc aaagtgctcc agttcggcaa gccgggccga aaatacgagt
     1141 acctgcggtg ggaagacttg atgcgcgaag tcgtgctcaa cggctcagat cgcagcctgc
     1201 cgatgctcgt gcgcaactcc gacgggcagg aagtctggca cgaaatccgt cccagcgatc
     1261 ggctgaaggc gattaaggcg ctcaacaatc gacccacgat cggcgttgct ccgcagaaga
     1321 cgcgccaagt ccgcgtccga agtgaggaac ccgtgtattt ggcggcgacg gttgcacccg
     1381 ctttggagga gaaggacgtc attgtggccg tcgcagagaa gcggctggag gccgattggc
     1441 aacttacgga gctgttggcc aaccggccga gcggaccgct ggcggtgacc gtttcccgga
     1501 tcaagcaggg accggacggc aaggagctca agccgcaagt caaggaagaa ttcgacgcgg
     1561 tgatccagcc gcgggcgatg cgcgagctgg gcctggtgat gaagctgggg ccggttgtcg
     1621 cggtgcgcga gaattctccc gcggcagtcg ccggtattca agtcgatgac gagatcgaga
     1681 cgctcgacgg ccaggacgtg ggcgatccct tgacgctggg ccagcgcatg ctgccggggg
     1741 acaatcccaa ccgcaccttc gaagtgggaa tccgccgcaa agaccgtggc ggcaagtgga
     1801 tcaccaagaa gcttgtcgtg catccagagc tgccgcagca gtatcagaac ctgtttccca
     1861 accacatcaa cggcggcccg gccgcggtgg aatcgatcgg cctggccgtg gacgtgctga
     1921 acgtggtaag cgccgtcacg ccgggcggcc cggccgacat cgcgggtctg ttgccgggcg
     1981 acgtcgtcac ccagtatcaa ctggtcgcgg cgagccagcc ggctgcggaa caggaaacca
     2041 ggtttctcaa gccggttctg gacgtggaca agcccttcga cccggtcgtt ctcgatcaac
     2101 tcaacaacaa ttggatcagc ctgctggccc ggatgcagtt gctcgatccc cagacgagag
     2161 tcaagctgac ctgggaccgc aatggcaaat cgacgagcga agtactccag ccgatcgact
     2221 ccaccaagtg gtgggacgaa tcgcggaacc tgtcgttcta tacccagaag gtaacccacc
     2281 gggcggagaa ttggcccgac gcattcagcc ttggcttgcg cgagacgagc gagcgcctca
     2341 aggaagtgct gcgggtgctg tcgagcctgg tgaccggccg gctgtcggcc acgaaccttt
     2401 cgggtccggc cggcattttc gttgccgccg ggagctttgc ctcggaaggc atcccttcgc
     2461 tgctgatctt cctgacgctc ctgtcggcga acctggccgt gctcaatttc ctgccgatcc
     2521 cggcccttga cggcgggcac atgctg
//
LOCUS       DASUGB010000326         8209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1425685, whole genome shotgun sequence.
ACCESSION   DASUGB010000326 DASUGB010000000
VERSION     DASUGB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425685"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFV87_06255"
     CDS             complement(<1..206)
                     /locus_tag="VFV87_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEX5103392.1"
                     /translation="MSVYLLATLDTKGAEAEFVRDRLVELGVPVELVDTSCAGRGGDR
                     GAAVSAAAEQAARLVGEAFAGGKV"
     gene            complement(262..1059)
                     /locus_tag="VFV87_06260"
     CDS             complement(262..1059)
                     /locus_tag="VFV87_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5103393.1"
                     /translation="MRTNPVKEKLRKGEVSFGTWLSLGDLYATRVLARMGFDWLTLDI
                     EHSAIDWSQATTIFAAVADAGCVPLARVPEGSHHYIKRVLDAGAFGIVVPMVDTVEQA
                     RIAVAAAYYPPKGNRSVGGGMHSMNFAATAGEYYERANDNNLVVLQTESPRGVENAEG
                     IYSLPGVDAIFIGPNDLRFQMRTSEGTFPTAHEHEAAIQRVIAAGKKVKCPTGIHAMD
                     PQSAIDRAKQGMQFIAIGSDLRMMSQKAQEILDVIRPEGTGKDVVRY"
     gene            complement(1082..1378)
                     /locus_tag="VFV87_06265"
     CDS             complement(1082..1378)
                     /locus_tag="VFV87_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX5103394.1"
                     /translation="MAKVGEGQTPKNVSRAAVFHPEFREDLRYWVQTDRKTALRALDL
                     VEAILRDPFAGIGKPEPLKHLAAGVWSRRLTQEHRIVYLVSHDRIDFLQARYHY"
     gene            complement(1362..1646)
                     /locus_tag="VFV87_06270"
     CDS             complement(1362..1646)
                     /locus_tag="VFV87_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5103395.1"
                     /translation="MIQTTYSTARANLASLLDQVAGNREVAIIRRRGAEDVALVSAAE
                     LSSLQETAHLLRSPKNAQRLLTALNRALARRLKPRSVARLRREVGLGEGR"
     gene            complement(1753..3243)
                     /locus_tag="VFV87_06275"
     CDS             complement(1753..3243)
                     /locus_tag="VFV87_06275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX5103396.1"
                     /translation="MPIPVACTGCGKRFQAPDRGAGKRVPCPSCQTTISIPAHYVPAE
                     EEEYRLAEEEPRPAPPPVRPVSVMPAAAPPPPVPRAIPSSPRPAPAPAAPAQHAPPPG
                     FSPPPAGATAHDAALESAPGGWVPPTKSSTPTWLRHLHWCLALATIPLAISIFLPGES
                     NEELIARILSSIKAEQTASEPAADSPTESEPETATDDSDLEELLGYGSIDDLLMSLPG
                     RKIQGAFLARDSSLHWLFALVSVAAYMTFFVFLASDNSAKAWHLLAIGTFTATIGILL
                     LVIVQVLAMIGGGRGILGLIALSYTMALDPEMGFIPSFIGFTLGVGLCEELCKALPLL
                     IYYKIGNRQNWRGAFLWGLASGAGFGVSEGIMYSGDYYNGITGPGIYLVRFVSCVALH
                     AIWTGSVGIAINQRQYVLHEEQEHWAMYALGILQMIAVPMVLHGLYDTLLKKDMNFLA
                     LATAAASFGYLAWQISRLRTSDDEDERAAYVGQYIRSRIAEAGGQG"
     gene            3346..3753
                     /locus_tag="VFV87_06280"
     CDS             3346..3753
                     /locus_tag="VFV87_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103397.1"
                     /translation="MAWLWLLLAIVWASPWTLLGLFAGGIGLLTGGGVQRVGRVIEFH
                     GGWIDRVLRWVPIAGGAAAMTLGHCVIARTRADLNRSRRHELVHVAQYERWGLLFVPA
                     YFACSAWMWLRGCDSYLDNPFEVEAYSAESDTV"
     gene            3882..5087
                     /locus_tag="VFV87_06285"
     CDS             3882..5087
                     /locus_tag="VFV87_06285"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5103398.1"
                     /translation="MKWAGGLIAIVIACSVAGAQSRLEFERARHALVDEILVPGGIKN
                     ERVVKAMRDTPRHEFVAPTLRRQAYLDKGLPIGEQQTISSPLIVSQMTQALDPQPGDK
                     VLEIGTGSGYQAAVLSPLVKEVYSIEIVESLGKSAARTLKRLGYDNVFTKIGDGYQGW
                     KEHAPFDKIIVTCSPEKVPQPLVDQLADGGLLVIPVGERYSQTLYLMRKKGGKLESEA
                     LLPTLFVPMTGKAEEQRAVKPDPANPQLVNGSFEEQAFQSGAQPGWYYERQVAWTTDA
                     KSPDGQHCIEFRNNEPGVSAHLLQGFAIDGREVKELEITGWVKTANVLPGGHKDEAPY
                     FVVTLFNEQRKELENLVIGPFRGTSDWHVESKTFKIPPDAREGIFRIGLFGATGTAWF
                     DKLQIGKAK"
     gene            5234..5572
                     /locus_tag="VFV87_06290"
     CDS             5234..5572
                     /locus_tag="VFV87_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103399.1"
                     /translation="MVGDRLRRIYELAVSTKQLARFVVFGSFVTDKSEPNDVDIIMLM
                     EDAFDLSAVHAEASLIFQHLDAETHFGASIFWTRRHGAIGGEQAMIEYWQVRREGGLR
                     GIVEIVPEET"
     gene            5569..5775
                     /locus_tag="VFV87_06295"
     CDS             5569..5775
                     /locus_tag="VFV87_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103400.1"
                     /translation="MITNDQQLKVTQQRIAWFQDQVFHLRQTELNPVNFRAAASGFLA
                     EIDRMQLDVREYLGTHPTELAEAP"
     gene            5812..6300
                     /locus_tag="VFV87_06300"
     CDS             5812..6300
                     /locus_tag="VFV87_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103401.1"
                     /translation="MDVFSGTEIAELLTELGITHVVWTPDTATGPWEAALESSPALSL
                     VRVCREGEAWPLAAGLLLGGKSPLVVMQTTGLFESGDALRNVLYDLKLPIYSLIGVRN
                     WLNAGSKDTARAFTEPILEAWGVDRVWLTQPADKPKLAEHYRRCRAAGIPGMALLAEG
                     SP"
     gene            6297..6905
                     /locus_tag="VFV87_06305"
     CDS             6297..6905
                     /locus_tag="VFV87_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5103402.1"
                     /translation="MTTSQAMPVVEALQVLADLCCDGQIVVTNQGSARIWPKLRSELT
                     GRERALDFHYNPSTMGGAIPLALGLALAQPARDVVAVSGDGSLLMSLGSLVTVIGAGA
                     TNLTIVLLDNGIYEVTGGQQTPASSAVDYCGLARAAGFPSVGNFEQLEHWSASAAGAL
                     SQPGPRFLRLAVGTSPEEHLRFPTPPIAEQLAQLQRALRVEI"
     gene            complement(6921..7730)
                     /locus_tag="VFV87_06310"
     CDS             complement(6921..7730)
                     /locus_tag="VFV87_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103403.1"
                     /translation="MTDELRTSEKAGNRLTVGLLLLWMLAASCLFGIHTSDGLRYADQ
                     VRSMWTPDGLAEQTQRQIGRFFTIVDVSGWIVLPVYAVGLAGAFLLVWRRFRRGPPFP
                     SQAGHFVLITSGVFALSTEIWWAVWSSPRVQGDGAYYGTWHATIERWQFVPYALAFAS
                     IATALAFGHIPWKWRTVLGLGTTAMGLFIVGSLMRADVIDVRPYSLYDLVEWPAKFVL
                     AIAFLMSMAIAIAEVRSGPCHDFLHYAGFVVAPTLPLRLIALQFTANHFLT"
     gene            complement(7754..>8209)
                     /locus_tag="VFV87_06315"
     CDS             complement(7754..>8209)
                     /locus_tag="VFV87_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103404.1"
                     /translation="GLFSYDLTAEGNTLPGKSHGTLIAGAKSVDCRALCVGKSGTCWV
                     AVTESAPVTDDKGGSVNINLLHLVSHRPGEKEPTDHGPVAISNPDYTPMTGADGKTLP
                     FHGGQITLQDGAVTTKHVILGVSEGLDGNVNILALIPYTLLQVPNPRGP"
BASE COUNT         1508 a         2514 c         2685 g         1502 t
ORIGIN      
        1 tccaccttgc cccccgcaaa cgcctcgccg acaagcctcg ccgcctgttc cgcagccgca
       61 ctcaccgccg cgccccggtc gccgccgcgg ccagcgcagc ttgtatccac gagttcgact
      121 ggtactccca gctcaaccag ccgatcgcgg acgaactcgg cctccgcgcc tttggtatcg
      181 agcgtggcca gaagatagac gctcacgcaa acaccgtccc ctcgttccct gttccctgtc
      241 ccctgttccc tgtcccctcc cctagtaccg caccacatcc ttccccgttc cctccggacg
      301 gatcacatcc agaatctcct gggccttctg gctcatcatc cgcaggtcgc tgccgatggc
      361 gatgaactgc atgccttgct tggcgcggtc gatggcgctt tgcgggtcca tggcgtggat
      421 gccggtcggg cacttgacct tcttgccagc ggcgatgacg cgctggatgg ccgcttcgtg
      481 ttcgtgggcg gtggggaagg tgccttcgct ggtccgcatc tggaaccgca ggtcgttggg
      541 gccgatgaag atcgcatcga cccccggcag gctgtagatc ccctcggcgt tttccacgcc
      601 gcgcgggctc tcggtctgca ggacgacgag gttgttgtcg ttggcccgct cgtaatattc
      661 gcccgcggtg gcggcgaagt tcatcgagtg catgccgccg ccgacgctgc ggttcccctt
      721 gggcgggtaa taggcggcgg ccaccgcgat gcgggcctgc tcgaccgtat cgaccatcgg
      781 cacgacgatg ccgaaggccc cggcgtcgag cacccgcttg atgtaatggt gcgagccttc
      841 cggcacgcgg gccagcggca cgcagccggc gtcggcgacg gcggcgaaga tcgtggtggc
      901 ctgcgaccag tcgatggccg agtgctcgat atcgagcgtc agccaatcga agcccatgcg
      961 ggccaggacg cgggtcgcgt acaggtcgcc gagcgagagc caggtgccaa aactcacttc
     1021 gcccttgcga agcttttctt tgacggggtt ggtgcgcatg gaagacctga cgggacgcta
     1081 cctagtagtg atatcgggcc tgcaagaagt cgatgcggtc gtggctaaca agatacacga
     1141 tacggtgctc ctgagtcaga cgccgcgacc atacgcccgc ggcaaggtgc ttgagcggct
     1201 caggtttgcc gattccagcg aagggatcgc ggaggatggc ttccacgagg tccaaggcgc
     1261 ggagggccgt tttccggtcg gtttgaaccc agtagcggag gtcctcgcgg aactccggat
     1321 ggaacaccgc cgcacggctc acgttcttgg gagtttgccc ttcaccgacc ttcgccaagt
     1381 ccgacctccc gccgcagtcg cgccacgctt cgcggcttca gccgtcgtgc cagagcgcga
     1441 ttgagcgccg tcaggaggcg ctgggcgttc ttcggcgagc gcaagagatg ggcggtttcc
     1501 tggaggctgg aaagttccgc ggcagagacg agcgccacat cctccgcgcc gcggcggcgg
     1561 atgatggcca cttcgcgatt gcctgcgacc tggtccagaa gcgatgccag attcgcgcgg
     1621 gcggtcgagt aggtcgtttg gatcatggca ggtcgttgaa aagagggggc caaagacttg
     1681 tacagcaaaa tggtacaatt caagaaccgg ccagtcaagt tcgatcgcac ggctggatgc
     1741 ggtcgcaggg gctcagccct gcccgccggc ttccgcgatc cggctgcgga tgtattggcc
     1801 gacgtaggcc gcccgctcgt cttcgtcgtc gctggtccgc aggcggctga tctgccaggc
     1861 gagatagccg aagctggcgg cggcggtcgc cagggcgagg aaattcatgt ccttcttgag
     1921 cagcgtgtcg tacaggccgt gcagcaccat cggtacggcg atcatctgca ggatccccag
     1981 ggcgtacatc gcccagtgct cctgctcctc gtgcaggacg tattgccgct ggttgatcgc
     2041 aatgccgacc gagccggtcc agatcgcgtg cagggcaacg cacgagacga accgcacaag
     2101 gtaaattccc ggcccggtaa tgccgttgta atagtcgccc gagtacatga tcccttccga
     2161 gacgccaaag cccgcgcccg acgccagccc ccacaggaaa gctccccgcc aattctgccg
     2221 gttgccgatc ttgtaataaa tgagcagggg cagggccttg cacagctctt cgcacaggcc
     2281 gacgcccagc gtgaagccga tgaagctggg gatgaagccc atctcgggat cgagggccat
     2341 ggtgtacgag agggcaatca gcccgaggat gcctctgccg ccgccaatca tcgccagaac
     2401 ctgcacgatg accagcaaga ggatgccgat ggtggcggtg aacgtgccga tggcgagcag
     2461 gtgccaggcc ttcgcggagt tgtccgacgc caggaagacg aagaacgtca tgtacgcggc
     2521 gacgctgacg agcgcgaaga gccagtgcag cgacgagtcg cgggcgagga aggcgccctg
     2581 gattttgcgg cccggcaagc tcatcagcag atcgtcgatc gagccgtagc ccagcagctc
     2641 ttccaggtcc gagtcgtcgg tcgcggtctc cggttcgctt tcggtcgggg aatcggctgc
     2701 aggctcgctg gccgtctgct ccgctttaat ggagctgaga atgcgtgcga tcagttcctc
     2761 attcgattca cctggcagga agatcgagat cgccagcgga atcgtggcca gggccagaca
     2821 ccagtgcagg tggcggagcc aggtgggcgt ggagctcttc gtcggcggga cccagccgcc
     2881 gggggcggat tccagcgccg catcgtgggc agtagcgccg gccggaggcg gcgagaagcc
     2941 gggtggcggg gcgtgctgcg ctggcgctgc tggtgctggc gcgggccgcg gtgaactggg
     3001 aatcgcccgc ggtacgggag gcggcggcgc tgccgcgggc attaccgata ctggcctgac
     3061 cggcggcggg gccggccgtg gctcttcctc cgccagccgg tattcctcct cttccgcagg
     3121 cacgtagtgc gccgggattg aaatcgtcgt ctggcagctt gggcaaggga cgcgttttcc
     3181 cgcgccgcga tcgggcgctt ggaagcgctt gccgcaaccg gtacaggcaa cgggaatcgg
     3241 catggatcgt tgcggcaaga gacgagagag gcccgtgggc ggtattatga gtgagtggaa
     3301 gggggctgtc caatcggctc gctacagacc ggtattcgag tttcgatggc ctggctttgg
     3361 ctgctactgg caatcgtttg ggcgtcgccc tggacgctgt tagggctctt cgcgggcggc
     3421 attggtctct tgaccggcgg cggtgtgcag cgggtggggc gggtgatcga attccacggc
     3481 ggctggatcg accgcgtgct ccgctgggtg ccgatcgccg gcggggcggc ggccatgacg
     3541 ctgggccact gcgtgatcgc tcgcacacgg gccgacctca atcgcagccg ccggcatgag
     3601 ctggtccacg tcgcccagta cgagcgctgg gggctgctct tcgtccccgc gtattttgcc
     3661 tgctcggcct ggatgtggct ccgcggctgc gactcgtatc tcgacaatcc gtttgaggtc
     3721 gaggcctatt cggctgaatc cgacaccgtt taacgcggag ccgcaggcga ctcgcgctgc
     3781 gcgaaccgcc ggcatcgcct gcgtctcgcc gctaaatgag tcgggcagcg ccgtggatga
     3841 tgtataattc gctggggcgg cacagccggg aggaatggac catgaaatgg gcaggcggac
     3901 tgattgcaat cgtgatcgcg tgcagcgttg ctggggcgca gtcgcggctg gagttcgagc
     3961 gggcccgcca cgcgctggtc gatgagatcc tcgttcccgg cggcatcaag aacgagcggg
     4021 tcgtcaaggc gatgcgcgac acgccgcggc acgaattcgt ggcccccacg ctccgccgcc
     4081 aggcgtatct cgacaagggc ctgccgatcg gcgagcagca gacgatcagc tcgcccctca
     4141 tcgtttcgca gatgacgcag gccctcgacc cgcagccggg cgacaaggtc ctggaaatcg
     4201 gcaccggcag cggctatcag gcggcggtgc tcagcccgct ggtgaaggag gtctattcga
     4261 tcgagatcgt cgaatcgctc ggcaaatcgg ccgcacgcac gctcaagcgc ctcggctacg
     4321 acaacgtgtt cacaaaaatc ggcgacggct accaaggctg gaaggagcat gccccgttcg
     4381 acaagatcat cgtcacctgc tcgccggaaa aagtcccgca gccgctggtc gatcagcttg
     4441 ccgacggtgg attgctcgtg attcccgtcg gcgagcgcta ttcgcaaacg ctctatctca
     4501 tgcggaagaa aggcggcaag ctcgaatccg aagccttgct gccgacgctg tttgtgccga
     4561 tgaccggcaa agccgaagag cagcgggcgg taaaacccga tccggcgaac ccgcagctcg
     4621 tcaacggcag cttcgaggaa caggccttcc agagcggcgc tcagcccggc tggtattacg
     4681 agcggcaggt cgcctggacg accgacgcca agtcccccga cggccagcac tgtatcgagt
     4741 ttcgaaataa tgaacccggc gtcagcgctc accttttgca gggcttcgcc atcgacggcc
     4801 gggaggtgaa ggagctggaa atcaccggct gggtcaaaac ggccaacgtc cttcccgggg
     4861 ggcacaagga cgaggcgccg tatttcgtcg tcacgctctt caacgagcag cgaaaagagc
     4921 tcgaaaacct cgtcatcggc cctttccgcg gcaccagcga ctggcacgtg gaatcgaaga
     4981 ccttcaagat cccgcccgac gcccgcgaag gcatcttccg catcggcctg ttcggagcga
     5041 cgggcacggc gtggttcgat aagctgcaga tcggaaaggc gaagtaaccg ccatctccgc
     5101 cattgagtca taccaatcga gccgatggca attccggact tcaatgctga tggcgacctt
     5161 ccggccggca tccacctcgc gacgctggca caggtgcttg gctcggtttg gttcaggctc
     5221 cgtgcagcga cgaatggtcg gcgatcgctt gcgccgcata tacgaattgg ctgtatccac
     5281 gaagcaattg gctcgatttg tcgtgtttgg ctcgtttgtc acggacaagt ccgagccgaa
     5341 cgacgttgat ataatcatgt tgatggaaga tgcatttgac ttgtccgcgg tccatgccga
     5401 ggcatcattg attttccaac acctcgatgc agagacgcac tttggcgcga gcatcttctg
     5461 gacccgccga catggcgcga tcggcggcga gcaagcgatg atcgaatact ggcaggtgcg
     5521 ccgcgagggc ggattgcgcg gcatcgtcga gatcgtaccg gaggaaacat gatcaccaac
     5581 gatcaacagc tcaaggtgac acagcagcgg atcgcctggt ttcaagacca ggttttccat
     5641 ttgcgccaga ccgagctcaa cccggtgaat tttcgcgcgg ctgcctctgg attcttggct
     5701 gagatcgacc gcatgcagct tgacgtgcgc gagtatctcg gcacgcatcc aacggaattg
     5761 gctgaagcgc cgtgatgagt cgtgcgagcc aactagatct aacttgtcac catggatgtg
     5821 ttttcgggga ctgaaattgc cgaattgctg acggaactgg gcatcacgca tgtcgtgtgg
     5881 actcccgata cggcaaccgg accgtgggag gcggcactgg agtcgtcgcc ggccctctcg
     5941 ctggttcgcg tctgccgtga gggggaggcg tggccgctgg cggcggggct gctgctggga
     6001 ggaaagtcgc cgctggtcgt gatgcagaca acggggttgt ttgagtcggg cgacgcgctg
     6061 cggaacgtgc tgtacgacct gaagctgccg atttattcgc tcattggcgt gcggaattgg
     6121 ctgaacgccg ggtcgaagga cacggcccgc gcttttaccg agccaatcct ggaggcgtgg
     6181 ggggtcgatc gcgtctggct gacgcaaccg gctgacaagc cgaaactggc ggagcattat
     6241 cggcgctgcc gggccgcggg cattcccggc atggcgctgc tcgcggaggg ctcgccatga
     6301 cgacgagtca agccatgccg gtagtggagg cgctgcaggt gctcgccgac ctgtgctgcg
     6361 atggccagat cgttgtgacg aaccaaggct cggcgcggat ttggcccaag ctccgcagcg
     6421 aattgactgg cagagagcga gcgctcgatt tccactacaa cccctcgacg atgggcgggg
     6481 cgattccact cgccctcggc ctggcgctcg cccagccggc gcgcgacgtg gtcgccgttt
     6541 cgggcgatgg atcgctgttg atgagtctgg ggtcattggt cacagtcatc ggcgcaggcg
     6601 cgacgaacct cacgatcgtc ctgctcgaca acggcatcta cgaagtcacc ggcgggcagc
     6661 agacgcctgc ttcctccgct gtggattact gcggcctggc gcgggccgca ggctttccga
     6721 gcgttggcaa cttcgagcag ctcgaacatt ggtctgcgtc cgcagccggc gcgctcagcc
     6781 aacccggccc gcgattcctc cggctggcgg tgggaacatc gccggaggag catctgcggt
     6841 ttcccacgcc accaatcgct gagcaactcg ctcaattgca gcgcgcactg cgtgtggaaa
     6901 tataggccac agttccctcg tcaagtgagg aaatgatttg ccgtgaattg aagggcgata
     6961 agtcgaagag gcagagttgg cgcgactaca aaaccggcat aatgaaggaa atcgtggcag
     7021 gggccactcc ttacctccgc tatggcaatg gccatcgaca ttaggaacgc tattgcaagt
     7081 acgaatttcg caggccattc gacgaggtca tatagcgagt aggggcgaac atcgattaca
     7141 tccgcccgca tcaatgaacc aacaatgaag agtcccatgg ctgtcgtgcc gaggcccaat
     7201 acagttcgcc atttccaagg gatgtgaccg aacgccaatg ccgtcgcaat tgatgcgaat
     7261 gccaacgcat acggtacgaa ctgccagcgt tcgatcgtgg catgccaggt gccgtaatag
     7321 gcgccatctc cttgaactcg gggggaactc caaacggccc accaaatctc cgtggacaat
     7381 gcgaataccc ccgatgtgat caacacaaaa tgccctgcct gcgatggaaa cggtggtcct
     7441 cgtcgaaaac gtcgccagac caatagaaac gccccggcaa gccctacggc gtagacgggt
     7501 aatacaatcc aacccgaaac gtcaacgatt gtgaaaaatc ggccaatctg tctctgcgtc
     7561 tgttcggcga ggccgtctgg ggtccacatg ctgcgaacct ggtcggcata acggagtccg
     7621 tcgctggtgt gaatcccaaa caggcacgac gcggcgagca tccacaataa caagagtcct
     7681 acagtcagac ggttgcccgc tttctcagag gttcgaagtt cgtcggtcac tttacaccgt
     7741 gctacgcgtg tgattacggc ccgcgcggat tgggaacctg cagcagtgtg tagggaatca
     7801 gcgcgaggat gttgacgttg ccatccaggc cttcgctgac gccgagaatc acgtgctttg
     7861 tggtgacggc gccgtcctgg agcgtgatct ggccgccgtg gaagggcagc gtcttgccgt
     7921 cggcgcccgt catcggcgtg tagtcgggat tggaaatcgc aaccgggccg tggtcggtcg
     7981 gctccttctc gccggggcgg tggctgacga ggtgcagcag gttgatgttc accgagcctc
     8041 ccttgtcgtc ggtgaccggg gccgattccg tgacggcgac ccagcaggtc cccgacttgc
     8101 cgacgcacag cgcccggcaa tcgaccgatt tcgccccggc gatcagcgtg ccgtgcgatt
     8161 tgcccggcag cgtgttgcct tccgcggtca gatcatagga aaagagccc
//
LOCUS       DASUGB010000327         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1566488, whole genome shotgun sequence.
ACCESSION   DASUGB010000327 DASUGB010000000
VERSION     DASUGB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566488"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..895
                     /locus_tag="VFV87_06320"
     CDS             86..895
                     /locus_tag="VFV87_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5103405.1"
                     /translation="MPRFVIDTIDDPRLEVYRDLPQRNLTRHSGRFIAEGDKLVERLM
                     ASDFDLESVLAEPALAERYAPRLPAETPVFVLSKPLLQQTVGFRFHRGVLACGRRKKR
                     LTMSELLRQALDSQSATATWVVCPDVQDPTNLGSLIRSAAALGCSGVLLGSNCADPFS
                     RRVLRVSMGAALYLPIIESRDLAADLRELSSAGFEIVAAVLDRAAEPLATFQRPPRTA
                     ILFGSEGHGLSPEWLALADRCVTIPMHGGIDSLNVAVAAAVVLWQLQCVRP"
     gene            1091..2593
                     /locus_tag="VFV87_06325"
     CDS             1091..2593
                     /locus_tag="VFV87_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5103406.1"
                     /translation="MQRLILRCGGLVALFFTVTAAAEEPEQVDLATELKTRSADTILH
                     VYGDYWKRGKLAATAVDMMSRDPDPANRLKAYQVWQELTKRLAQERETAIHLALKNAD
                     PSVESLVRSVILTSADSGVRRVEFLLNWDEQHPPGRDCGVTSTGIFLSTVSASSLRPL
                     ANHENPKIRALAVAEFARRSREDYANIEEVRKSLFHADARRRVEAIQAHVQLGLSPAS
                     DARIEELLADDDADVRKAAIGYFGSRVNYERDRVLAALQHSDAEVRRIAADALGGVRP
                     ANEETIQKLSAVIADDADAQVAQAAISSLADLDYRNEAAVPAIARRLRECQDRQARLA
                     IIYCLDKIGPAAQSAVPDIRAVAADSEHSQLTIEHALWRITGEPEALLEAIRVALERA
                     VRENIKARLPDAQVRFAGELGPAAKPLVPLLVAHLDYALANPPEPGKASIWFHSRYNT
                     ICALGAIGPDAKDALQSLESFDQSEHAKPFDHKAAAEAIAAIRGMPPVQP"
     gene            complement(2659..2832)
                     /locus_tag="VFV87_06330"
     CDS             complement(2659..2832)
                     /locus_tag="VFV87_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5103407.1"
                     /translation="MRTRFLYWLRVVDGRFARRLCDEAPSAYKDIQAVMRALRELTRI
                     DRKLRPLLSHKGC"
     gene            complement(2966..>3352)
                     /locus_tag="VFV87_06335"
     CDS             complement(2966..>3352)
                     /locus_tag="VFV87_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5103408.1"
                     /translation="QPQPRGRIYTGDAAFKMRPQSPTLVGIDVAYVSAALRARTPKGS
                     KIIEGVPTVAVEILSPSDVHSDLSEKIQEYLDVGVELVWIVDPDFETVTAYTNDDEPQ
                     LFNRGQELTAEACLPGFQVRVAELFE"
BASE COUNT          623 a         1038 c         1043 g          648 t
ORIGIN      
        1 gggttcccgc cattgatcgc tataaccgct acagtcggac cttggataac gataggaacc
       61 gcacttctgc ggattagtgc gccccatgcc acgcttcgtc atcgacacca tcgacgatcc
      121 gcgactggag gtctatcgcg acctgccgca gcggaacctg acgcgccata gcggccgatt
      181 tatcgccgaa ggcgacaagc tcgtcgagcg gctcatggca agcgatttcg acctggagag
      241 cgtgctggcc gagccggcgc tggccgagcg ttatgcgccg agacttccgg cagagactcc
      301 cgtttttgtc ctttccaaac ccctgctgca gcaaacggtt ggattccggt ttcatcgggg
      361 cgttttggct tgcggacgcc ggaaaaagcg gctcacgatg agcgagctgc tgcgacaagc
      421 acttgactcg cagagcgcga cagcgacgtg ggtcgtctgc cccgacgtcc aggatccgac
      481 gaacctgggc agcctcatcc gttccgccgc agccctgggc tgcagcggcg tcttgctggg
      541 cagcaattgt gctgatcctt tctcgcgtcg cgtcctgcgg gtctcgatgg gcgcggcgct
      601 ctatctgccg attatcgaat cgcgcgatct ggcggctgat ttgcgcgagc tttcctcggc
      661 cggattcgag atcgtggccg ccgtgcttga ccgagcggcc gaaccgctag cgaccttcca
      721 gcgccccccg cgaacggcca ttctcttcgg cagcgaaggc cacgggctct ccccggagtg
      781 gctcgccctg gccgaccgct gcgttaccat ccccatgcac ggcggaatcg actccctcaa
      841 cgtcgccgtc gccgccgcgg ttgtgctctg gcagcttcag tgtgtccggc cctaggaaac
      901 tcgcggaccc tcggtcgccg cgcgccggcc aaatcgcgaa cacgggagtt tctgggtgaa
      961 aatctcgacg cgaatgaata tcgattcgtt catcgactcc gaaccgcctt tccgctagca
     1021 ttcgtacctc tctggcagga gttcgctgct attggcggct ctcgctcttt ccatgcaaag
     1081 gagggactca atgcaaaggc ttatcctgcg gtgcggtggc ttggtagcac tgttcttcac
     1141 ggtgactgcg gcggctgaag agccggagca agtcgatctt gccaccgagc tgaagacgcg
     1201 gtctgcggac accatcctgc acgtctatgg cgactattgg aagcggggga agctggcggc
     1261 gacggcggtg gatatgatgt cgcgcgaccc cgatcccgcc aaccgtttga aggcgtatca
     1321 ggtttggcag gaactcacga agcgactggc ccaggaacgc gaaaccgcaa tccatctagc
     1381 cctaaagaac gcggacccca gcgtggaaag cctcgtacga tccgtgattc tgacctccgc
     1441 agactctgga gtcaggcgcg tggaatttct gttgaattgg gacgagcagc atcctcccgg
     1501 ccgcgactgc ggcgtgactt ccacgggcat tttcctgtcg acggtgtcgg cctcatcgct
     1561 ccgacctttg gcgaatcatg agaatccgaa gattcgggcc ctagcggtgg ccgagttcgc
     1621 ccgtcgttcg cgagaagact atgccaatat cgaagaggtt cgcaagtcgc tgtttcatgc
     1681 ggatgcacgg cggcgcgtcg aagcgattca agcgcatgtc caactcggtt tgagtccggc
     1741 gagcgacgcc cggatcgagg aattgctggc ggacgacgac gcggacgtcc gcaaggcggc
     1801 catcgggtat tttgggagcc gggtgaacta cgagcgcgac cgcgttctgg cggcactgca
     1861 gcattccgac gcggaggtcc gtcggattgc tgccgatgcg ctcggcggag tgcggccagc
     1921 gaacgaggag acgattcaga aactctcggc ggtgatcgcc gacgatgcgg atgctcaggt
     1981 ggcccaggcc gccatctcga gcctggcaga cctcgactac cgcaacgaag cggcggtccc
     2041 ggcgatcgcg cggcgcctgc gcgaatgcca ggaccgtcaa gcacgcctgg cgatcattta
     2101 ttgcttggac aagatcggcc ccgccgcgca atcagccgtt ccggacatcc gcgcggtggc
     2161 tgcggactcg gagcactcgc aattgacgat cgagcacgcc ttgtggagaa tcaccggcga
     2221 gcccgaggct ctgttggaag cgatccgcgt cgctttggaa cgcgccgttc gcgaaaacat
     2281 caaggcgcga ctgcctgatg cccaggtgcg attcgctggt gaattggggc ctgcggcgaa
     2341 gccgttagtg cccttgctgg tggcgcatct cgactacgcg ctggccaatc cacctgagcc
     2401 gggaaaggct tcgatatggt tccattcgcg gtataacacg atctgtgccc tgggagccat
     2461 tggaccggat gccaaggacg ctttgcaatc cctggaatcg ttcgaccaat cggaacatgc
     2521 caagccgttt gaccacaagg cggctgccga agcgattgcc gcgattcgag gcatgccgcc
     2581 cgttcagccg taggtgctct taccaggcag cgtgctgaag gcgcgttgat cgcgccaaaa
     2641 ccgctgtaat tcggccaatc agcagccttt atggctcagc agtggacgca gcttgcggtc
     2701 gatgcgggtc agctcgcgca gggcccgcat gacggcttgg atgtccttgt aggccgaggg
     2761 tgcttcgtcg cacagccggc gggcgaagcg gccgtcaacc acacgcagcc aatatagaaa
     2821 cctggttcgc acgccaggtg tgagattcga acagaaggac gccaagaccg caaagaagag
     2881 aaagacggcc atgcaagatg ttcgacagga ctttttcctg ctcctttgcg cccttggcaa
     2941 tcgtctgctc actttcttcc ctgcgctact cgaacagctc cgccacgcga acttgaaatc
     3001 ccggaaggca tgcctccgcc gtcagctctt ggccgcgatt gaatagctgc ggctcgtcgt
     3061 cgttggtgta agccgtgacg gtctcgaaat cgggatcgac aatccagacg agttcgacgc
     3121 cgacgtcgag atactcctgg atcttttccg agagatcgga gtgaacgtcc gacggggaga
     3181 ggatttccac agccacggtg ggcacacctt cgatgatctt gctgcccttt ggcgtccggg
     3241 ctctgagtgc agcggaaacg taggccacgt caatgccgac cagcgtcgga ctctgcggcc
     3301 gcatcttgaa cgccgcatca cccgtataga tgcgcccgcg cggctgcggc tg
//
LOCUS       DASUGB010000328         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1982752, whole genome shotgun sequence.
ACCESSION   DASUGB010000328 DASUGB010000000
VERSION     DASUGB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982752"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFV87_06340"
     CDS             complement(<1..257)
                     /locus_tag="VFV87_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103409.1"
                     /translation="MKRHLLSVRTSAVILLFTFVGVSVARAAEFDQAKLSAIAPRMQQ
                     FVDEGVVSGVVAVVGNAQGVVQLDAVGLQDLEAKQPMPRDA"
     gene            complement(257..1057)
                     /locus_tag="VFV87_06345"
     CDS             complement(257..1057)
                     /locus_tag="VFV87_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103410.1"
                     /translation="MSPSRDARESRWGSADGGARSTGSSSLQVAVESFASNNCGMSST
                     ANELRVLVANPPTDLVVHFDAVTEMHEHRQFLNLAAAVAMSQTFREFHPLATALGLVF
                     LDDANTSNYHAYITIGPLASAILYLSHDGDTRAVFPALADYRADLQAAYEEGEYSFDA
                     HDEPPIQARDQAALTARIAQLIEEDEEAELLVLLASWNASSEIELKQLVRHPNFFVAE
                     ANGNTIARLARPELLPFANILTGHPHSQAANAGKRAMAAIQGTEKRNP"
     gene            complement(1054..>2413)
                     /locus_tag="VFV87_06350"
     CDS             complement(1054..>2413)
                     /locus_tag="VFV87_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103411.1"
                     /translation="VLLLGVIGVLAILLLMPSRGLARPAANRVAAPPGAPASAAARAP
                     TASASAGVQAGGTQWVAYEDAEKGFQAAFPGSQPQPIDPLAQIEDPQQRGLAEAMMKE
                     WTVLGVTHGGRKYTLSAMPFDLGSIPPATYLDRMSAGLELIHQGFSVEPQPAADAPAP
                     QRDFVLKKDDAGKLLRVVVGRGRLYQLLIEGDAGLGLGDPVAKEFFERFHCETVSSAP
                     ATSATETASNSDSDAGTGQPKPETEPRAGELPAEEPPTEIEWRPAQGTTFPFTIAFPG
                     VTPAEVDPLAPLPEQGRATFEENWQRDSLVVESYLAEAGDRRYAVTAFRHPEIPAEES
                     RRLIGQMVNLTSTYVRYLYPADRGRGYSAGQPLTRWQQWTTTSHTLEDGSKAIVRQAQ
                     LGYYGFVARVEGPAGMDDLDPLALRFINSLQPPGDAAMPPIDDKPPARPSAKTKTKQK
                     SK"
BASE COUNT          426 a          773 c          781 g          433 t
ORIGIN      
        1 tccgcatccc tcggcatcgg ctgcttcgct tccaggtcct gcaggccgac tgcgtcaagc
       61 tgcaccacgc cctgcgcgtt gccgacgacc gccaccacgc cagaaacgac cccctcgtcc
      121 acaaactgct gcatccgcgg agcgatggcg gagagcttcg cctggtcaaa ttcggcggcg
      181 cgggcaaccg aaacgcctac gaacgtgaaa agcagaatga ctgcggaagt acggacggaa
      241 agcagatggc gcttcattac gggttcctct tctctgtgcc ttgaatggcc gccattgctc
      301 gtttacctgc attagcggct tgggaatggg ggtgaccggt aagtatgttt gcgaatggca
      361 acaactccgg gcgcgcaagc cgtgctatcg tgttgccgtt cgcttcggcg acgaaaaagt
      421 tgggatggcg aacgagctgt ttcagctcga tttcactgga tgcgttccac gatgccagca
      481 ggacgagcag ttcagcttcc tcgtcctcct cgatgagctg agcgatacgc gccgtaagcg
      541 ccgcctgatc gcgggcctga atgggcggct cgtcgtgggc gtcaaacgaa tactcaccct
      601 cctcgtatgc ggcctgcaag tcggcccgat agtccgctag cgcgggaaac accgcgcggg
      661 tgtcaccatc gtgcgacaag tacaatatcg cactagccaa tgggccaatc gtgatatagg
      721 cgtgataatt cgacgtgttc gcatcgtcca ggaaaaccag cccgagcgcc gtggcaagcg
      781 ggtggaattc gcgaaacgtt tgactcatcg caaccgccgc ggccaggttc aggaattggc
      841 ggtgctcgtg catttccgtg acggcatcaa aatgcaccac caaatccgtc ggcgggttgg
      901 cgaccagaac tcgtagttcg tttgccgtgc tcgacatgcc gcaattgttg ctcgcgaacg
      961 attcaaccgc cacctgcaga gaagaggaac ccgtactcct ggcgccgcca tcagcacttc
     1021 cccaccggga ttcgcgggca tcgcgtgaag ggctcatttg ctcttttgtt tcgtcttcgt
     1081 tttcgccgat ggcctggcgg gcggcttgtc atcgatcggc ggcatcgcgg catcgccggg
     1141 gggctgaagg ctattgatga accgcagcgc cagcgggtcg agatcgtcca tcccggccgg
     1201 cccctcgacg cgcgccacga agccgtaata ccccaactgg gcctgccgga caatcgcctt
     1261 gctcccatcc tccagcgtgt gggaggtggt ggtccactgc tgccaccggg tcaagggctg
     1321 gcccgccgaa tatccgcggc cgcggtcggc cggatacagg taacgcacgt aagtcgatgt
     1381 caggttcacc atctggccga tgaggcggcg cgactcctcg gcggggattt ccggatggcg
     1441 gaaggccgta acggcatagc ggcgatcgcc ggcctcggcc aagtacgatt cgacgaccag
     1501 gctgtcgcgc tgccagtttt cttcaaaagt cgcccgacct tgctcgggaa gcggtgccag
     1561 cgggtcaact tctgcgggcg taacgccggg aaaggcgatg gtaaagggga acgtggtgcc
     1621 ctgcgccggc cgccattcga tttcggtggg aggctcctcc gcaggcaact cacccgcgcg
     1681 gggctccgtc tcgggcttcg gctggccggt gccggcgtcc gaatccgaat tgctggcggt
     1741 ctcggtggcg ctggtagccg gggcgctgga aaccgtttca caatggaagc gctcgaaaaa
     1801 ctccttggcc accggatcgc ccaagcccag gccggcatcg ccttcgatga gcagttgata
     1861 cagccgcccg cggccgacca cgacgcggag cagcttcccg gcgtcgtcct tcttcaagac
     1921 gaagtcgcgc tgcggcgcgg gtgcatccgc ggccggctgc ggttcgaccg aaaagccctg
     1981 gtgaatcagt tccaatccgg ccgacatccg gtccaagtag gttgcaggcg gaatgctgcc
     2041 gaggtcgaag ggcatcgcgg atagcgtgta cttgcggccg ccgtgcgtga cgcccaggac
     2101 ggtccactcc ttcatcatcg cctcggccag cccgcgctgc tgcgggtcct cgatctgggc
     2161 gagcgggtcg atcggctgcg gctggctgcc gggaaaggcg gcttggaagc ccttttcggc
     2221 gtcttcgtag gcgacccact gcgtgcctcc agcctgaacg ccggcgctgg cagaagctgt
     2281 gggcgcccgc gccgcggccg acgccggggc cccaggcggc gcggcgacgc ggttggcagc
     2341 cgggcgggcc aacccgcggc tgggcatcaa gagcagaatc gccaacacgc cgatcacgcc
     2401 gagcagcagc acg
//
LOCUS       DASUGB010000329         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_732251, whole genome shotgun sequence.
ACCESSION   DASUGB010000329 DASUGB010000000
VERSION     DASUGB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732251"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFV87_06355"
     CDS             complement(<1..691)
                     /locus_tag="VFV87_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103412.1"
                     /translation="MTADDFLNDLAGQNLVPGEIIESLRRQVAKAARPVAPQTLAKLL
                     VEKGHLTAVQGQRLLGAAPAAAAKKPAAALEPLGPLDDLGLAPLDELPAPPQPAAPAK
                     PAAQPAAKPAAAPAAAKPPAKPAAKAKAPAPTDDLQPLDDLQPLDDLAPLDAAPLDAL
                     PSGDLLGAPAGVGSPVGFSDPFAAPSGGGAAVVAAADPLAEIPATALAPAGVTAPVSA
                     AMPAQAPAKKSS"
     gene            complement(715..1683)
                     /locus_tag="VFV87_06360"
     CDS             complement(715..1683)
                     /locus_tag="VFV87_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103413.1"
                     /translation="MATTDPLFPDSALQPAAEPMEDAHAAPGEDVVQAATQAEAAATG
                     EATAEEAPQPRRGGCMLVALGLVAGVIVLGGVAGGAWWMLSGGSSAEPSTAAASDKPT
                     APEPAGPPPALTFAEAPTAKETTAAAPTLASRIERIPGLVSAYETELLTAVETAPADV
                     IVTASGRQSVDSVSEIPRHERWQIEFPPGNTIESYTRQLEYFKMEIGLIGGSSEIEYL
                     VNISDPVPTVRKGPASEEQRLYLIWQRGSMREADEQLATRARVSSAGKIFAHFCPEAL
                     ETDLAQREDAFAKQNGTERIRKTIFGVKANEFGGFQFYVIDQKAVE"
     gene            complement(1777..>2258)
                     /locus_tag="VFV87_06365"
     CDS             complement(1777..>2258)
                     /locus_tag="VFV87_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103414.1"
                     /translation="KVDELTKSRLQRSAKGESSLLGGSPEHYQKLPDPYDDSQPLEAR
                     ARSYLHANCSICHVEAGGGNAQMQLEYTTELAKMGIIDSPPLHHRVEIPDARLIAPGD
                     PARSVLLHRLSRRGAGTGQMPQLATYLVDQQAVELFEDWIKVVQPPVQSEKDSASSP"
BASE COUNT          329 a          765 c          776 g          388 t
ORIGIN      
        1 tgctcgattt cttggccggg gcctgggccg gcattgcggc gctcaccggc gctgttacac
       61 cagcaggcgc tagggccgtg gccgggattt cagccagcgg atcggcagcg gctacgactg
      121 cagccccgcc gccagacggc gcggcgaagg ggtcggagaa gcccacgggc gagccaacgc
      181 ccgccggcgc gccgagcaag tcgccgctgg gcagcgcatc gagcggagcg gcgtccagcg
      241 gcgcgagatc gtcgagcggc tgcaggtcat ccagcggctg caagtcatcc gtcggcgcgg
      301 gcgctttcgc cttggctgcc ggtttcgcgg gtggcttagc ggcagcaggt gcggccgccg
      361 gtttcgcggc cggttgtgct gcaggcttcg ccggtgcggc cggctgcggc ggcgcgggaa
      421 gttcgtcgag gggcgcaagg ccgagatcat cgagcggacc gagcggctcc agcgcggccg
      481 cgggcttctt cgccgccgcg gccggcgcag cgcccaagag ccgctgcccc tggaccgctg
      541 tcagatgccc cttctcgacc aacagcttcg ccaacgtctg cggcgcgacg ggcctggcgg
      601 ctttggccac ctgccgccgc aagctctcga tgatttcgcc gggaacgaga ttctgccccg
      661 cgaggtcgtt caggaagtcg tctgctgtca tcgcccgccg ccggctggaa gtcgctactc
      721 cacagccttt tgatcgatca cgtaaaactg aaacccgcca aattcgttcg ccttcacgcc
      781 aaagattgtc ttgcgaatcc gctcggtgcc gttctgtttg gcgaaggcgt cctcgcgctg
      841 agccaggtcg gtctccaggg cctcggggca gaagtgggcg aagattttgc ccgccgacga
      901 aacccgcgcc cgcgtggcga gctgctcgtc ggcctcgcgc atcgagcccc gctgccagat
      961 caggtacagc cgctgctctt cgctggccgg gcccttgcgg acggtcggca ccgggtcgga
     1021 aatgttaact aggtactcga tctcactgga gccgccgatg aggccgatct ccatcttgaa
     1081 gtattcgagc tgccgcgtgt acgactcgat cgtgttgccc ggcgggaact cgatctgcca
     1141 ccgctcgtgg cgcggaatct cgctgacgct gtccaccgac tgccgcccgc tggccgtaac
     1201 aatcacgtcc gcgggggccg tttcgaccgc cgtcagcagt tccgtctcgt aggccgatac
     1261 taggcctggt atccgctcga tccggctggc cagggtgggc gcggcggccg tggtttcctt
     1321 ggccgttggc gcctcggcaa aagtcagcgc cggcggaggc cccgcgggct ccggcgcggt
     1381 cggtttgtcg ctggcggcgg ccgtactggg ttcggccgac gagccaccgc tcagcatcca
     1441 ccacgcaccc cccgcgacgc cgcccagcac aattacaccg gcaaccagcc ccagcgcaac
     1501 gagcatacac ccgccgcgcc gcggctgggg cgcttcttcg gcagtcgctt cgccggttgc
     1561 ggcggcttca gcctgcgtcg ccgcctgcac tacgtcctcg ccaggcgcag catgagcatc
     1621 ctccatcggc tcggcggccg gttgtagcgc gctatcggga aacagaggat cggtggtagc
     1681 catcgagacg tgttggcgac gaatgagcct ccagtatcgg tcgccgcaac ttgcatcgca
     1741 accggaaagt cctcctatca gccgtcaagc cgcgagctag gggctcgacg ccgagtcctt
     1801 ctccgactgg acgggaggct ggacgacctt aatccaatcc tcaaacagct ccactgcctg
     1861 ctgatcgacc aggtaggtgg cgagctgggg catttggccg gtgcctgcgc cgcggcggct
     1921 gaggcggtga aggagaacgg agcgggccgg gtcgccgggg gcgatcagcc gggcatccgg
     1981 aatctcgacc cggtggtgca gcggcgggga gtcgatgatt cccatcttgg ccagctcggt
     2041 cgtgtattcg agctgcatct gcgcgttgcc gccgccggct tcgacgtggc agatcgagca
     2101 attcgcgtgc agatacgacc gggcccgggc ctcgagcggc tgcgagtcgt cgtagggatc
     2161 gggcagtttc tggtagtgct ccggcgagcc gcccaagaga gacgattcgc ccttggccga
     2221 ccgctgcagg cgcgatttcg tcagttcgtc gaccttgc
//
LOCUS       DASUGB010000330         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_872130, whole genome shotgun sequence.
ACCESSION   DASUGB010000330 DASUGB010000000
VERSION     DASUGB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872130"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="rny"
                     /locus_tag="VFV87_06370"
     CDS             <1..1074
                     /gene="rny"
                     /locus_tag="VFV87_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5103415.1"
                     /translation="ETELANEVGTRISKHEKKIAAICDQKTRETLITSLQRYAASHTA
                     ETTTSTVDIPSDEMKGRIIGREGRNIRAFEKATGVDVIIDDTPGVVIVSGFDPVRREI
                     ARQTLNKLIADGRIHPTRIEEIAADTQKEIEGFIQKKGEEAAQEVNIHGLNPKVLYML
                     GRLHFRTSYSQNVLRHSIEVAFLTGLLAEMIGLDGDLGRRCGLLHDIGKAADHELEGG
                     HPKIGADLLKRNGEKTEVVHAAFGHHDEIVTDYPYTMLVATADACSAPRPGARRESLE
                     RYIKRMEELESIACGFPGVEQAFAIQAGRELRVIASAKDTDDSKAAKICRDIAKAFEE
                     QLTYPGEIKVTVVRESRFSEVAR"
     gene            1168..1983
                     /locus_tag="VFV87_06375"
     CDS             1168..1983
                     /locus_tag="VFV87_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX5103416.1"
                     /translation="MRILLIGDIVGKPGERIVTQCLPGLRRRESLDLVIANAENAAEG
                     SGLAPSQYRRLLAAGVDCITLGDHIYRRSEICEILQTERNIVKPANFPAAAPGKTWSV
                     ASATDGTPVAVVSLLGRVFMRPVDCPWAAIDRVLAEIPADVKVRVIDFHAEATSDKQL
                     MGRYLDGRVTAVLGTHTHVTTADEQIFPGGTAFQCDVGMTGPHESILGRRIERVLETT
                     VTFQPTHFEVASGDVRLSGTVVEADPATGRAIAVRRLVVTHDEAERLSSQCET"
     gene            complement(2126..2473)
                     /locus_tag="VFV87_06380"
     CDS             complement(2126..2473)
                     /locus_tag="VFV87_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103417.1"
                     /translation="MARKVVNRKELRAEAEAAEAIGKTSADGTAPAEAKGKKAAKPKG
                     EKKAPVKRKSRAKSQEPERRRLFWGVFNQSLKRVALFDHTQKKAAEAKATELSGTGKS
                     PHFVMKVKEAIAE"
BASE COUNT          492 a          811 c          790 g          456 t
ORIGIN      
        1 gagacggaac tggccaatga agtcggcacg cggatctcga agcacgaaaa gaaaatcgcc
       61 gccatctgcg accagaagac ccgcgagacg ctaattactt cgctgcagcg ctacgccgcc
      121 tcgcacaccg ccgagacgac caccagcacc gtcgacattc ccagcgacga gatgaaggga
      181 cgcatcatcg gccgcgaagg ccgcaacatc cgcgccttcg aaaaagcgac cggcgtcgac
      241 gtcatcatcg acgacacgcc cggcgtggtg atcgtcagcg gcttcgaccc ggtgcgccgc
      301 gaaatcgccc gccagacgct caacaagctg atcgccgacg gccgcatcca tcccacgcgg
      361 atcgaggaaa tcgccgccga tacgcaaaag gaaatcgaag gcttcatcca gaagaagggg
      421 gaagaggcgg cccaggaagt aaatatccac ggcctcaacc ccaaggtgct gtatatgctc
      481 gggcggctcc atttccgcac cagctacagc cagaatgttc tgcggcacag catcgaggtg
      541 gcgttcctca ccggcctgtt ggccgagatg atcggcctgg acggcgacct gggccgccgc
      601 tgcggcctgc tgcacgacat tggcaaggcg gccgaccacg agctggaagg aggccatccc
      661 aagatcggcg ccgacctgct caagcgaaac ggcgaaaaaa cggaggtcgt gcacgccgcc
      721 tttgggcatc acgacgaaat tgtcacggat tatccataca cgatgctcgt ggcgaccgct
      781 gatgcctgca gcgccccgcg ccccggggcg cgccgcgagt cgctcgaacg ctatatcaag
      841 cggatggaag agctggaatc gatcgcctgc ggcttccccg gcgttgagca ggccttcgcc
      901 attcaggcgg gccgcgagct gcgggtgatc gccagcgcca aagataccga cgactccaag
      961 gcggcgaaga tctgccgcga cattgccaag gcgttcgaag aacagcttac gtatccgggc
     1021 gagatcaagg tgaccgtcgt gcgcgaatcg cgattttcgg aggtggctag atgagtatga
     1081 gagtgtgagc gtaatagagt gtggctgagc gacgacgctc cgttgcactc tgtcactctc
     1141 gcactcccgc accctcacac gcgtcccatg cgcatccttc tcatcggcga tatcgtcggc
     1201 aaaccgggcg agcggatcgt cacgcagtgc ctgcctgggc tgcggcggcg agaatctctg
     1261 gatctggtca ttgccaatgc cgaaaacgcg gccgaaggct ccgggctggc gccctcccaa
     1321 taccggcggc tgttggccgc gggagtggac tgcattacgc tcggggacca catttaccgc
     1381 cgctcggaaa tatgcgaaat cctgcaaacc gaacgcaaca tcgtcaagcc ggcgaatttt
     1441 cccgccgccg cgccgggcaa aacgtggtcc gtagcctcgg caaccgacgg cacgccggtc
     1501 gccgtggtga gcctgctggg gcgggtgttc atgcggcccg tcgattgccc ctgggcagca
     1561 attgatcgag tcctggcgga gatccctgcg gatgtgaagg ttcgcgtcat cgatttccac
     1621 gccgaggcca ccagcgacaa gcagctgatg ggccgctatc tcgacggcag ggtgacggcc
     1681 gtcctgggga cgcacaccca cgtgacgacg gccgacgagc agatttttcc cggcggtacg
     1741 gcattccagt gcgacgtcgg catgacaggt ccgcacgaga gcatcctggg ccgcaggatc
     1801 gagcgggttc tcgaaaccac cgtcaccttc cagccgacgc atttcgaggt ggccagcggc
     1861 gacgtgcgcc tctcgggaac cgtcgtggaa gccgatccag cgaccggacg agcgattgca
     1921 gttcgccgcc tggtggtgac gcacgatgag gcggaacggt tatccagcca atgcgagacg
     1981 tgagccgcac agcacttatt tcaatccggt ctggaccttg ccggtgacca ccctgccttt
     2041 tagggtaatg gtgatcgaca gcgcgtcgcc agaaccgccg aaccatttga gctcaacgtc
     2101 gctttacggg cccacatatt gcctgtcatt ccgcgatcgc ttcctttacc ttcatcacga
     2161 agtgcgggct cttgccggtg ccggaaagtt ccgtggcctt ggcttcagcg gccttttttt
     2221 gcgtgtggtc gaagagggcc acccgcttga gcgactggtt gaatacgccc caaaacaggc
     2281 ggcgacgttc cggctcctgg cttttggccc ggcttttgcg cttgaccggg gccttcttct
     2341 cccctttggg cttggcagct tttttcccct tggcttcggc cggcgccgtg ccgtccgcgg
     2401 atgttttccc gatggcctct gccgcttcgg cttcggcgcg taattccttg cgattgacga
     2461 ctttacgtgc catggcggac gagattcctg cggggcgcac ggcgaagtgg gtggagcgaa
     2521 gtggccgtat cttaatgcat catccgcgc
//
LOCUS       DASUGB010000331         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1982976, whole genome shotgun sequence.
ACCESSION   DASUGB010000331 DASUGB010000000
VERSION     DASUGB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982976"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFV87_06385"
     CDS             <1..193
                     /locus_tag="VFV87_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103418.1"
                     /translation="AGTKKEFKYRISVCCCESRETKYQLRMVVAAVPDKTEAARLLWR
                     EADELNRIFAAIFRNTDDD"
     gene            251..1159
                     /locus_tag="VFV87_06390"
     CDS             251..1159
                     /locus_tag="VFV87_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5103419.1"
                     /translation="MAKSVLSRYLDPDVLSRVADRHLDPKGLVIGNLAGAHKSPLAGF
                     AVEFAGHREYVPGDDPKHVDWRVYFTREKYFVKQYELETNFVCHLVLDVSASMRYGEG
                     REQKLLYAAQMASTLGYSVIHQSDKVSLFTFDDHVRGFVTPSNSMAQVVRMTEHLDEI
                     EAVQKTAMAECLNDIAGRLGRREIVMIFSDFFTDLDQLEPVLQRLRYAKHEVVLFQVM
                     HHDELAFEMDGMVKFVGLEIPEELLAQTDDLRRAYLQAVSQFNDQLEEIAHRNGCERV
                     LCDTRRGVSEVLIDYLNRRSMLALAR"
     gene            1285..2457
                     /locus_tag="VFV87_06395"
     CDS             1285..2457
                     /locus_tag="VFV87_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5103420.1"
                     /translation="MRYSFRLAEVVNHVPDPRRRPGTIKAICDYTGLDRHQVAALLKN
                     EVKYVPLDALSKLCGYLIERGFAKADELPGRLFGVEPEHFWELLARRRRLELCLGVRR
                     GDAAGTLDDAWVVASDSVLLGEILNGATTLTSAPISPSDGDGQPVAIGTVRPHPEHVH
                     QSLVWSPEQATVEECQRMARGVYEDFCEVAGDKALVCLGSIKSNPVVELLLATAFGCE
                     PFLSQDDLPASERRSCPIFLRYRDDDPHPLSCCGGQRAARGQSSNKPGIYYETAAGKW
                     ECCQWDPTTHDAAFVFYIHRESQGRMEMALGGFSGRATRLLARVLARRGEEFWPPVYS
                     DEGIQIGAFVVKFALTGHSAPDRAFTGADQHAEETIIRLDSEVIARRMQQHVEAAT"
     gene            2498..2884
                     /locus_tag="VFV87_06400"
     CDS             2498..2884
                     /locus_tag="VFV87_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5103421.1"
                     /translation="MDLNGKVALVTGGTMGIGAAIAVDLARRGADVAIAARNLGGPAE
                     QTRELILASGRRCLLIGADCAVAGDCSRAVAETVKQLSRLDVLVHNAGGPAPGRIEDV
                     TPELWRHTMDLHVNANFYLERISKPS"
     gene            complement(2870..3648)
                     /locus_tag="VFV87_06405"
     CDS             complement(join(2870..3303,3303..3648))
                     /locus_tag="VFV87_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX5103422.1"
                     /translation="MEPDFELTDERWNLISDLFHEPPRRPQGGRPPAPARGCFEGILW
                     ILRSGARWKDLPKHFPSSTTCWRRHKEWTEAGVWKTAWARLVRALDRRGRVKHEESFA
                     DGTFSPAKKGAKRLGKTKRGKGTKIMVFTDAEGLPLGADTASASPHEVTLIEPLLETR
                     VLRRKPQRLVYDEAADSDPLRERLQQRGIELVCRHRRNRVKPPTQDGRKLRRFRRRWK
                     VERSISWLQNFRRLVTRYEFHAHLFHGFVQLACLIVVLGRF"
BASE COUNT          727 a         1147 c         1186 g          720 t
ORIGIN      
        1 ggctggcacc aagaaggagt tcaagtaccg gatcagcgtc tgctgttgcg agtcacgcga
       61 gacaaaatac caactgcgga tggtcgtggc ggccgtacca gacaaaaccg aagctgcgcg
      121 attgctttgg cgagaagcag atgaactaaa caggattttc gcagccatat ttcgcaacac
      181 tgatgacgac tgacccactc tcattggtaa ttgggacttg gtcattggtc atttgccagt
      241 aagtaataca atggcaaaga gcgttttgtc ccgctatctc gaccccgacg tgctcagccg
      301 cgtggcggat cgccatttgg atccgaaagg actggtgatc ggcaacctgg cgggggcgca
      361 caagtcacca ctggcggggt tcgcggtcga gttcgcgggg catcgcgagt atgtgccggg
      421 ggacgatccc aagcacgtcg attggcgcgt ctatttcacg cgggaaaagt acttcgtcaa
      481 gcaatacgag ttggagacga acttcgtctg ccacctggtg ctcgatgtca gtgcctcgat
      541 gcgctacgga gaggggcgcg agcagaaact gctgtatgcg gcccagatgg cttcgacgct
      601 cggctactcg gtgattcatc aaagcgataa ggtgtcactg ttcacgttcg acgaccacgt
      661 gcggggtttc gtcacgccca gcaactccat ggcccaggtc gtacggatga ccgagcacct
      721 ggacgagatc gaggcagtgc agaagaccgc gatggccgaa tgtttgaacg acatcgccgg
      781 acgcctcggg cggcgcgaaa ttgtgatgat cttcagcgac ttctttaccg acctcgatca
      841 gctcgaaccg gttctgcagc gcttgaggta cgcgaagcac gaggtggtgc tcttccaggt
      901 gatgcaccac gacgaattgg cgtttgaaat ggacggcatg gtgaagttcg tcggcctgga
      961 aatccccgag gaattgctcg cgcaaaccga cgacctccgt cgggcctatc tgcaagccgt
     1021 cagccaattc aacgatcagc ttgaagagat cgcccaccgc aacggctgtg agcgggtgtt
     1081 gtgcgacacc cgtcgcggcg tcagcgaggt cttgatcgac tacctcaatc gccgcagcat
     1141 gctggcgctg gcccgctgac ggcccaatga ttgcgttctc gtaagaaatc tgctagaacc
     1201 cttacagcgt ttcatctccc gttaagtgcc gctttgcggc agatagcgga agatgaactt
     1261 tcgcaagctg gggagggcgg ggagatgcga tactcgtttc gtctggcgga agtcgtcaac
     1321 cacgtccccg atccgcggcg ccggccgggc acgatcaagg ccatctgcga ttacacgggc
     1381 ctcgaccgcc accaggtggc cgccctgctc aagaacgaag tcaagtacgt cccgctggac
     1441 gccctttcca agctctgcgg ctacctgatc gaacgaggtt ttgcgaaggc ggacgaactt
     1501 cccggccggc tcttcggcgt ggagccggaa cacttttggg agctcttggc tcggcggcgg
     1561 cggctggagc tgtgcctggg ggttcgccgc ggcgatgctg caggcacgct cgacgacgcc
     1621 tgggtcgtcg cctccgattc ggtgctcttg ggcgaaatcc tcaacggcgc gacgacgctg
     1681 acgagcgccc caatctcgcc atcagacggc gatggacagc ccgtagcgat agggacagtg
     1741 cggccgcacc cggagcacgt gcatcagtcg ctcgtttgga gcccggagca agctacggtc
     1801 gaagagtgcc agcggatggc ccgcggcgtc tatgaagact tttgcgaagt cgccggtgac
     1861 aaggccctcg tctgtctggg cagcatcaag agcaatccgg tcgtcgaatt gttgctcgcc
     1921 accgctttcg gctgcgagcc gtttctctcg caggacgatc tgccggcgag cgagcggcgc
     1981 agttgtccga tttttctccg ctaccgagac gatgacccac acccgctttc ctgctgcggc
     2041 ggccagcggg ccgcacgggg gcaatcctcc aacaagccgg gcatctacta cgaaacggca
     2101 gccggcaaat gggagtgctg tcagtgggac cccaccacgc acgatgcggc attcgtattt
     2161 tacatccacc gtgaatcgca agggcgaatg gagatggcgc tgggaggttt ctcagggcgg
     2221 gcgacgcggc tgttggcccg cgtgctcgcc cgccgcggcg aggaattctg gccccccgtt
     2281 tattccgacg aaggcattca gatcggcgcg ttcgtcgtca agtttgcact gaccggccat
     2341 tcggccccgg atcgcgcctt caccggggcc gatcaacacg ccgaggaaac gatcatccgg
     2401 ctggacagcg aagtcatcgc ccgccggatg cagcagcacg tggaagccgc gacgtaagcg
     2461 gattcgccaa cctcattccc atctgaagga acctcgcatg gacctgaacg gcaaagttgc
     2521 gcttgtcact ggcgggacaa tgggcattgg cgccgcgatc gccgtggacc tcgcccgccg
     2581 tggggcggat gtcgccatcg cggcgcgcaa cctgggcggc cccgcggagc agacgcgcga
     2641 gctgattctg gcgagcggcc gccgctgctt gctgatcgga gctgattgcg ccgtggccgg
     2701 cgactgcagc cgcgcggtcg cggagaccgt taagcaattg agccgcttgg atgtgctggt
     2761 tcacaatgcc ggcggccccg cccccggaag aatcgaggac gtcacgcctg aactgtggcg
     2821 gcacacgatg gacctgcacg tgaacgccaa tttctacctc gagcgcattt caaaaccgtc
     2881 ctaggacgac gatcaagcag gccagttgta cgaagccatg aaacaggtga gcgtgaaatt
     2941 cgtaccgcgt gaccagtcgc cggaaattct ggagccaact gatggaacgc tccactttcc
     3001 agcgacggcg gaagcgccgc agcttccggc cgtcttgggt aggcggcttc acgcgattgc
     3061 ggcggtggcg acaaaccagt tcaatgccgc gctgttgcag tcgttccctc aacggatcgc
     3121 tgtcggcagc ctcatcatag accagacgct gcggcttgcg gcgcagcacc cgtgtttcga
     3181 gcaggggctc gatcagcgtc acttcgtgag gactggcgct ggccgtgtcg gctcccaggg
     3241 gaaggccttc ggcatcggta aacaccatga ttttcgtgcc tttgccgcgc ttggtcttgc
     3301 ccaccttttc gccccttttt ttgcgggtga aaacgtgccg tcggcaaacg attcctcgtg
     3361 cttcacacgg ccgcggcgat cgagcgcacg caccaggcgc gcccaggccg ttttccaaac
     3421 gcccgcctcg gtccactcct tgtggcgacg ccagcacgtc gtggaggagg ggaaatgctt
     3481 gggcaagtcc ttccagcgcg cgccgctccg gagaatccag agaatgcctt cgaagcagcc
     3541 gcgcgccggc gctggcggac ggccgccctg ggggcgacgc ggtggttcgt ggaatagatc
     3601 ggaaatcagg ttccaccgct cgtcagtgag ttcaaagtcg ggttccatcg tggaccccgc
     3661 gggcgattcc accgccccac gggatcggtt tgccatgtgt gacctccttg gggtttcact
     3721 ccatacggca aacctccaac tgaggtttag ctagcaaaaa cacgttttga aatacgctct
//
LOCUS       DASUGB010000332         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1288407, whole genome shotgun sequence.
ACCESSION   DASUGB010000332 DASUGB010000000
VERSION     DASUGB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288407"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFV87_06410"
     CDS             <1..277
                     /locus_tag="VFV87_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103423.1"
                     /translation="LPPAQVIPQLPSPEPQAALAERQRLLYGLALGLLDVQPGTAEYW
                     MCDDAQSGELRYGRWRRDALAGAKWIAQDLNNPLARHPETGFRLEIR"
     gene            274..2358
                     /locus_tag="VFV87_06415"
     CDS             274..2358
                     /locus_tag="VFV87_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5103424.1"
                     /translation="MSQSEIEYRKQLERMTLSTYRETNSFPAVVFATVVVLGLYLANR
                     YGFLNYEWLARPRRMLLESGWVPYGIIWWCLFGLQYLSQIFFRRLAPLANAATLEQDV
                     IQTARRDGLQSQDTHRILVDRTRDGRRGYFTDRFLRLFNLYQTSFDLAAVQRLRDEIL
                     ENDEHTHQLGFSAVRCSEWAMPLFGFLGTVIGIGQAIGSLDQGMDFDPAGHLTTKPAL
                     IKQSFEGMALAFETTLEGLIGLLLVGTIHAFLRKRLEQQLARAQPYFTETIEKTENRP
                     ARVVVEELTVLNLDMGLLRSEMEQLREDLHERHLHHREIEAMVETVIDQDPRFQPIKE
                     ILFSPIVEFESAFAGVSTDLEKFIQGKVGKVPWSFLAASAAPVGNWSAALVEYGNQTG
                     LCFFNVLEPGALEWVALGGQRFDRLQMCSESAALLRESQQGMLHRIERTQGIPQSIRR
                     LQQANDLALPYLHDGQALGAVLANLDPTQPSVEYFSLNGVAGTQKAAVPTGGGAAWRH
                     AALAPSGAALVLAEQQPAKGSDCSLLHMPWQPPQASKDKNKPASGPAPLPGQSNRIVL
                     SGIKGLRQTLWISDSELLLLDDTGKVWLFDLSRETPVLLEHERWKPAADTVLIAGHNR
                     WFARHAGGHLSMWKVWRTGRLHDYSSGTPLHVAKFAASHAGTLQEGRLLWSAVGQRLA
                     VWRFPEYQSDSR"
     gene            2379..>3098
                     /locus_tag="VFV87_06420"
     CDS             2379..>3098
                     /locus_tag="VFV87_06420"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEX5103425.1"
                     /translation="MRRRTTESAETLFSPAVDVLANTVLIMFIYSILFIVTKGRDPDP
                     LTFLSCQPPPCICDFPYDYTLPTAGGLGERKFRLVKGKLPAGLVLDARTGRVSGTVAA
                     THAAGQSDVTIAVQDESGEQQQDLSFGIWSTIVPADREAAKVRLTAEDPQLPAARAGL
                     PYVAALGHAGGVGTLAWKLVGDGLPPASGLALNEGRLEGTPQTPGVYEFEIEAACGAG
                     ELMYGGSKLAWEGTADRRKYTL"
BASE COUNT          542 a          960 c         1044 g          552 t
ORIGIN      
        1 gctgccgccg gcccaagtga ttccgcaact tccgtcgcca gagccgcagg cggcgctggc
       61 cgagcggcag cggctgctgt acggcctggc gctgggcctg ctcgacgttc agccgggaac
      121 ggcggagtac tggatgtgcg acgacgcgca gtcgggcgag ctccgctatg ggcgctggcg
      181 gcgcgacgcg ctggccggcg cgaaatggat cgcccaagac ctgaacaatc ccttggcccg
      241 gcatcctgag acagggtttc gattggagat tcgatgagcc agagcgagat cgagtatcgc
      301 aagcagttgg aaaggatgac tctctcgacc tatcgagaga cgaattcttt tcccgcggtc
      361 gtctttgcca cggtcgtagt tcttggcctc tatctggcaa atcgttacgg ctttctgaac
      421 tatgaatggc tcgctcgtcc gcggaggatg ctgctggaaa gcggctgggt cccgtacgga
      481 atcatttggt ggtgcctgtt tggcttgcag tacctcagcc agatcttttt tcggcgtctg
      541 gcgccgctgg cgaatgccgc gacgttggag caagacgtga ttcaaacggc ccgccgcgac
      601 ggcctccaaa gccaggacac gcaccggatt ctggttgacc ggactcggga cgggcggcgc
      661 ggctatttca ccgatcgatt cttgcggctg ttcaacctct atcaaacgag tttcgatctg
      721 gcggcagtgc agcggctgcg ggatgagatc ctggagaacg acgagcacac gcatcagctc
      781 ggcttctccg ccgtccgctg ttcggagtgg gccatgccac tgttcggttt tctggggacg
      841 gtgatcggca tcggccaggc gatcggcagc ctggaccagg gcatggattt cgaccccgcg
      901 gggcatctca cgaccaagcc cgccctgatc aagcagagct tcgagggaat ggccctggcc
      961 ttcgagacga cgctggaagg gctgatcggc ctgctcctcg tggggacgat ccacgccttc
     1021 ttgcggaaac gtttggagca gcagctcgcg cgcgctcagc cgtatttcac cgaaaccatt
     1081 gaaaaaacgg agaatcggcc ggcgcgagtt gtcgtggaag agttgaccgt cctgaacctg
     1141 gacatgggcc tcttgcggag cgagatggag caacttcgcg aggacctgca cgagcggcac
     1201 ctgcaccacc gcgaaatcga ggcgatggtc gagacggtga tcgatcagga cccgcgtttc
     1261 cagccgatca aggagatcct gttttcgccg atcgtcgaat tcgagtcggc gttcgcgggc
     1321 gtctcgaccg acctcgagaa gttcattcag gggaaagtcg gcaaggttcc gtggagcttt
     1381 ctggcggctt cggcggcgcc ggtcggcaac tggagcgcgg ccctggtcga atatggcaat
     1441 caaacggggc tgtgcttctt caacgtgctg gagccggggg cgctggagtg ggtcgcgctc
     1501 ggcggccagc gcttcgaccg cctgcagatg tgcagcgaga gcgccgcttt gctgcgcgaa
     1561 agccagcaag gaatgctgca ccgcattgaa aggacgcagg gcattccgca aagcatccgg
     1621 cgactgcaac aggccaacga tctggcgctt ccctatctcc acgatggtca ggccctggga
     1681 gcggtcctcg ccaacctcga tccgacgcag ccaagcgttg agtatttttc actcaacggc
     1741 gtggcgggca cgcagaaggc tgcagttccc accggcggcg gcgccgcctg gcggcatgcc
     1801 gccctggccc cgagcggcgc ggcgctggtc ctggccgaac agcagccggc caaggggagc
     1861 gactgctcgc tgctgcacat gccttggcag cctccgcagg cctccaaaga caagaacaag
     1921 ccggcgagcg gaccggcgcc tctgcccggc cagtcgaacc gcatcgtcct gtccgggatc
     1981 aagggcctgc ggcaaacgct gtggatctcc gacagcgagt tgctcttgct cgacgatacc
     2041 ggcaaggtct ggctgtttga cctgagccga gaaacgcccg tgctgctgga gcacgagcgg
     2101 tggaaacctg ccgcggacac ggtgctcatc gccggacata accgctggtt cgcccgccac
     2161 gccggcggcc atctctcaat gtggaaagtg tggcggaccg gccggctgca cgactattcg
     2221 agcggcacgc cgctgcacgt cgcgaaattc gccgcctccc atgcgggcac tttgcaggag
     2281 ggccgcctgc tctggtcggc ggtcggccag cgcctcgccg tgtggcggtt ccccgaatat
     2341 cagagcgaca gccgatagcc ttttgcgagg aacgttttat gcgtcgtcgg acgacggaat
     2401 ctgctgagac gctgttttcg cccgcggtgg acgtgctggc gaacaccgtc ctgatcatgt
     2461 tcatctactc gatcctgttc atcgtgacga aggggcgcga tcccgatccg ctcacgttct
     2521 tgagctgcca gccgccgccg tgcatttgcg attttcccta cgactacacg ctcccgacgg
     2581 ccggcggcct gggcgagcgg aagttccgcc tcgtcaaggg caagctgccg gcggggctgg
     2641 tgctggatgc aaggacgggg cgcgtctcgg gcaccgttgc cgcgacgcat gcggccggcc
     2701 agagcgacgt gacgattgcc gtgcaggacg aatcgggcga gcagcagcaa gacctgtcgt
     2761 tcggcatctg gtccacgatc gtccccgccg accgcgaagc ggccaaggtt cgcttgaccg
     2821 cggaagatcc gcagcttccg gcggcgcggg ccggcttgcc ttacgtggcg gcgcttggcc
     2881 atgccggcgg cgtcggcacg ctggcgtgga agctcgtcgg cgacggcttg ccgccggcca
     2941 gcggactggc gctcaacgaa ggccgtctgg aagggacgcc gcaaacgccc ggcgtctatg
     3001 aattcgaaat cgaagccgca tgcggtgcag gagagctgat gtacggcggg agcaagctgg
     3061 cctgggaagg cacggcggac cggcggaaat acacgctg
//
LOCUS       DASUGB010000333         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1288467, whole genome shotgun sequence.
ACCESSION   DASUGB010000333 DASUGB010000000
VERSION     DASUGB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288467"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VFV87_06425"
     CDS             complement(<1..1505)
                     /locus_tag="VFV87_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103426.1"
                     /translation="MLTRDEFAQAALIQLGARGETTAVYDAASFSIWTRPNENSEFSG
                     HSQLDRWYEKYQAAAPQGRCDEAIAEFVEHYFDNLRSAQSADLPNKARLIPLVRSRFD
                     FEVSALRSAVKNQGAPADVMNLLVHQPLAEHLDIYLSEDLPDGYRHLDRHVLDQLGLS
                     WDQALRLALWNLPQIDPGFGLDQPFAQQPQGHWSYIAPPSELFTCWLLFPERIRRLPV
                     KGRHVAFLPKVDRLAITGSEEIEVVNVLAKMTVESLRNSPGRPVTAIPFILEPAGWKP
                     WLPPARHPLYWTIKELHIIARGTMYGPQCKLLKEHYLRAEGDDAPHVAEYTGSEFEPF
                     RGQPILSDWCLWTQWVHTILPRTEAVVLLQLLNPEELNANPNAKEQFGEQITITWKQL
                     VDYLGPRLKKLDYYPERYQVAGDDFPEGDDWNHLKAAGVKLPLEGAPEEPTEPLPKLT
                     PPTAATTSSPAPPAPSQLARPFSPPQESKPPPEPSLWPLAVLALFALTCLGG"
     gene            complement(1539..>2263)
                     /locus_tag="VFV87_06430"
     CDS             complement(1539..>2263)
                     /locus_tag="VFV87_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103427.1"
                     /translation="KIAGVENPRVWLSGTPVPAAGEIPLDVQPGSNDVLIRLDGGRAV
                     ALVSLTLQARGNVSIVLPEKLDGSLLAERLRSAAAAGSGEALSAEFAGLDWPAEAKTG
                     DAAKGRVLFGSLGCSKCHAITSDQKGGGAPSLAEAWRRFTVPQMVESLLLPSKQIAGP
                     FRGTTLRTADGEVLNGLVVGESKEQLELLQPDGSRRAISTADIEDRKFSDLSPMPFGL
                     VKTPDELRDLLAYLQLQSPVPP"
BASE COUNT          411 a          721 c          732 g          399 t
ORIGIN      
        1 ccgcccaggc acgtcaaggc gaacaaggca agcaccgcca acggccacag cgaaggctcg
       61 ggcggcggct tcgattcctg cggcggtgaa aatggcctgg ccaactgcga tggcgctgga
      121 ggggccgggc tgctggtcgt cgccgcagtc ggcggcgtaa gcttgggcaa cggctcagtc
      181 ggctcctccg gtgcgccttc gagcggcaat ttcacgccag cggccttcag gtggttccaa
      241 tcgtccccct ccggaaaatc gtcccccgca acctgatacc gctcgggata atagtccagc
      301 ttcttcagcc gcggaccaag atagtcgacc aactgcttcc aggtgatcgt gatctgctcg
      361 ccaaattgct ccttggcatt gggattggcg ttcagctcct cgggattgag aagctgcagg
      421 aggacgactg cctcggtccg cggcaggatc gtgtgcaccc actgcgtcca caagcaccag
      481 tcgctcaaga tcggctggcc gcgaaacggt tcgaactccg agcccgtgta ttccgccacg
      541 tgcggtgcgt cgtcgccttc cgcccgaagg tagtgctcct tgagcagctt gcactggggc
      601 ccgtacatgg tcccccgcgc gatgatgtgc agttctttga tggtccaata cagcggatgt
      661 cgggccggcg gcagccaggg cttccagccg gccggttcga gaatgaatgg aatcgcggtg
      721 acgggacggc ccggagaatt tcgcagcgat tccaccgtca tcttggccag cacgttgacg
      781 acttcgatct cctcggagcc ggtgatcgcc agccgatcga ccttgggcag aaatgccacg
      841 tgccggccct tcacgggcag tcgccggatt ctttcgggaa acaggagcca gcacgtgaac
      901 agctcgctgg gcggggcgat gtaactccag tgaccctgcg gctgctgggc gaagggctga
      961 tcgagaccga agccaggatc gatctgcggc aggttccaca gcgccagccg cagggcctgg
     1021 tcccacgaca gaccaagttg gtccagcacg tgccggtcca agtgccgata gccgtcgggc
     1081 agatcctccg acaggtaaat gtccaagtgt tcggccagcg gctggtggac cagcagattc
     1141 atcacgtcgg ccggggcgcc ttggttcttc accgccgaac gcagcgcgga gacctcaaaa
     1201 tcgaaccgcg agcggacgag cgggatcagc cgggccttgt tggggaggtc tgcggactga
     1261 gcgcttcgca ggttgtcaaa gtaatgctca acaaactccg cgatcgcctc gtcgcagcga
     1321 ccttggggcg cggccgcctg gtatttctcg taccagcggt cgagctgaga atgcccgctg
     1381 aattcggagt tctcgtttgg cctcgtccag atcgagaaac tggcggcatc atagaccgcc
     1441 gtcgtctcgc ctcgcgcacc gagctggatc agggcggctt gggcaaattc atcgcgagtg
     1501 agcatggaca caccaacccg acgcgcaagc gagggaactc acggtggcac cggcgattgt
     1561 aactgaaggt aggccagcaa atcacgcaac tcgtcgggcg tcttgacgag cccaaagggc
     1621 atcggcgaga ggtcggagaa cttgcgatcc tcaatatcgg cagtcgaaat ggcccgccgc
     1681 gagccgtccg gctgcagcaa ttcgagttgc tctttggatt cgcccaccac cagtccgttc
     1741 agcacctcgc catcggcggt tcgcagagtc gtgccacgga agggcccggc gatttgcttg
     1801 ctcggcagca gcagcgattc gaccatttgc ggaaccgtga accgccgcca ggcttcggcc
     1861 aggctcggcg cgccgccccc tttctggtcg gaggtgatcg catggcattt gctgcagccc
     1921 agcgagccga agagcacgcg gcctttggcg gcgtcgcctg ttttggcttc cgctggccaa
     1981 tcgagccccg caaactccgc actgagtgct tcgccgctac ccgctgcggc tgcggatcgc
     2041 aggcgctcgg cgaggagcga gccgtcgagc ttctcgggca gaacgatgga gacgttgccg
     2101 cgggcttgca gcgtgagcga aacgagcgcg accgccctgc cgccatccag ccggatcaag
     2161 acgtcattgc tgccgggctg cacatcgagg ggaatttcgc cggcagcggg gacgggcgtg
     2221 ccgctgagcc acacccgcgg attctcgacg ccggcgatct tca
//
LOCUS       DASUGB010000334         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1149313, whole genome shotgun sequence.
ACCESSION   DASUGB010000334 DASUGB010000000
VERSION     DASUGB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149313"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFV87_06435"
     CDS             <1..779
                     /locus_tag="VFV87_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5103428.1"
                     /translation="LPSQLEEALRELAALGLVSSDTFAAVRAIGGSRDKAKAALRRYG
                     RMKVHRPTSPVGRWSLFPGSVDPPDRAGQVERWCRQVLERWGVVFRDLLAREPAAPPW
                     FELVRVLRRLELRGEIRGGRFIAQVGGEQFALETAVSRLRDLRDAAPSDDWSLISAAD
                     PLNLSGVITPTARIPAMHKNALVIQRGRCVAAKIAGRIEFLAEIDPNQQFLIRKSLQV
                     GRKVHPAAVPSVPEPHTFRDRHRSSAPDPTRLHSRRRLGY"
     gene            complement(773..>3956)
                     /locus_tag="VFV87_06440"
     CDS             complement(773..>3956)
                     /locus_tag="VFV87_06440"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5103429.1"
                     /translation="FVSGLCALIFQTAWLREFRLIFGASTPASAAVLAIFMGGLGLGN
                     AVLGRRVDRAASPLKVYALFELGISAAAAASPFLLECVRSLYFSLGGQESLGISGASM
                     VRLLLSTLVIGVPTFLMGGTLPAAARAATSAADRARVSVGWLYGLNTIGAVVGALAST
                     FVLLEMLGNRETLWAAAAANIVNAALAWRLAGQWSAAPAASAKRRTGGRHRPDADEPA
                     KQSDLMEGPLEAPSWLIYAAAGLVGFAFLLMELVWYRLLGPILGGTTFTFGIILAVAL
                     AGIGIGGALYPLLYRARLPTLRDFALTCAGEALAIGLPFAAGDRLAILAAVLRGLEYF
                     GFAGQASGWVIVAGIVIFPAALISGLQYPLLISLLGRGWKDVGKQVGQATAFNTLGAM
                     AGSLAGGFGLLPLLSAPGAWKLTVALLAGLSLASLGIGLRTGRQAVQTASSLLAVLIA
                     ALCLLATGPTAVWRHSHIGVGRAQMPDMTMNALKAWSNAARRSIEWEADGQESSVAVS
                     TNIGVTFIVNGKSDGNALMDAGTQIMFPMIGALIHPQPARALVIGLGTGESAGWLASL
                     PSMQQVDVVELEPAIVNVARLCAPLNHDVLHHRKVRVIFNDGREVLQTLPAKYDLIAS
                     EPSNPYRAGVANLYTKEFYESVEARLAPRGLFVQWLQGYEIDRVAVRTVLCTLHEVFG
                     HVEIWASRPNDLVLVCSRDPLDYPLPLLQSRIKEPAMREALRVGWRTTHLEGALAHFV
                     ANERYTAAVAHQRLGWINTDNRNVLEYSFARTVGRSLGFSVEGMREEARRQNANRPLA
                     IDAGVDWEAVEDEKIAFHAGSGDRPLPAGLFAGERERRAAAVALIAQGDMAGGTVTWN
                     QQSRAPTGLAEHLLLAMAYASTKDARAEPLIEKIRESSPLDADAIAGVLAVERQQYSE
                     AGRLLTQVFQTLRTNPTGDLRAIELALIRSTLVVERDPSQAQRLYRALSQPFAVYQFD
                     LRRRLSRWICAQHAGDAAMLETLAEFEPHVPWDRTFLQVRYAVYRRMQDPRAAQAERD
                     LREFMSWMPEQFVLEPEGAVLGAARQAAGQ"
BASE COUNT          721 a         1344 c         1265 g          626 t
ORIGIN      
        1 tgttgccctc gcagctcgaa gaggccctgc gggagctcgc ggccctggga ttggtttcct
       61 ccgacacgtt cgcggcggtg cgggcgattg gcggctcgcg cgacaaggcc aaggcggctc
      121 tccgccgcta tggacggatg aaagtgcatc ggcccacctc gcccgtcggc cgctggtcgc
      181 tatttcccgg cagcgtcgat ccaccggacc gggcgggcca ggtcgagcgc tggtgccggc
      241 aggtgctcga gcggtggggc gtggtgtttc gcgatttgct ggcccgcgag ccggccgcgc
      301 ccccctggtt cgagctggtc cgcgtgctgc ggcggctgga gctgcggggg gaaatccgcg
      361 gcggccgctt catcgcccag gtcggcggcg agcagttcgc cctggaaaca gccgtcagcc
      421 ggctgcgtga cctgcgcgac gcggcgccga gcgacgactg gtcgctcatt tccgctgccg
      481 atccgctgaa cctgtccggc gtcatcacgc cgacggcgcg gattcctgcg atgcacaaaa
      541 acgcgctcgt gatccagcgg ggccgctgtg tcgccgccaa gatcgccggg cgaatcgagt
      601 tcttggccga gatcgatccc aaccagcaat tcctgatccg caaaagcctg caggtcggcc
      661 gcaaagtcca tcccgccgct gtcccgtcgg ttcccgagcc ccacactttc cgcgatcgcc
      721 accggtcctc cgcacccgat cccacgcggc ttcacagccg tcggcggctg ggctattgac
      781 ccgccgcttg acgcgcagca cccaggacgg ctccttccgg ctccaagacg aactgctccg
      841 gcatccagct catgaattcg cgcaagtcgc gttctgcttg cgctgcgcgc ggatcttgca
      901 tccgccgata gacagcatag cggacctgaa gaaacgttct gtcccacggc acatggggct
      961 caaactcggc cagcgtctcg agcattgccg cgtcgccagc gtgttgcgcg cagatccatc
     1021 gcgacaacct tcgccgcaaa tcgaactgat aaactgcgaa tggctggctg agtgcccgat
     1081 acagccgctg cgcctggctg ggatcgcgct cgacgaccag cgtcgatcga atgagagcga
     1141 gctcgattgc gcggaggtcg cccgtcggat tggtccgcag ggtctgaaaa acctgggtga
     1201 gcaacctgcc ggcttcggaa tattgttgtc gttcgacggc cagcacgccg gcaatcgcat
     1261 ccgcatccag cgggctggat tcgcgaatct tttcgatcag cggctcggcc cgggcgtcct
     1321 tggtgctggc ataggccatc gccaggagca ggtgctcggc gaggcccgtc ggcgcccggg
     1381 attgttgatt ccatgtgacg gtcccgccgg ccatatcgcc ctgagcgatt agggccaccg
     1441 ctgcggcacg tcgttcgcgc tccccggcaa agaggcccgc gggcaggggc ctgtctccgg
     1501 agccggcatg aaaggcgatc ttctcgtctt cgacggcctc ccaatccacg cccgcgtcga
     1561 tcgccaacgg ccggttcgcg ttttggcgac gagcttcttc ccgcattccc tccaccgaga
     1621 atcccaggct gcggccgacc gttcgcgcaa agctgtattc cagaacgttg cgattgtcgg
     1681 tattgatcca ccccagccgc tggtgggcca cggctgcggt gtagcgctcg ttggccacaa
     1741 aatgggccag cgcaccctcg aggtgcgtgg tccgccagcc gactcgcagg gcctcacgca
     1801 tggcgggctc cttgatccgg gactgcagca ggggcaaggg atagtccagg ggatctcgcg
     1861 aacagaccaa caccagatcg ttgggccgtg aagcccaaat ctccacgtgc ccgaacacct
     1921 catgcaacgt gcagagcact gtccgcaccg cgaccctatc gatctcgtat ccctgcagcc
     1981 attggacgaa aagtccccgc ggcgccagcc gagcttcgac cgattcgtag aactccttcg
     2041 tgtagaggtt ggcaactcct gcccggtagg gattggaggg ttcggaggcg atcaggtcgt
     2101 atttcgcggg caacgtttgc aacacctccc gtccatcgtt gaaaatcacg cgcaccttcc
     2161 gatggtggag cacatcgtga ttgaggggag cgcagagtct cgctacattc acgatcgccg
     2221 gctccaattc aacgacgtcg acctgctgca tggatggcag cgacgccagc cacccggccg
     2281 attcgcccgt ccccaagcca ataaccagcg cccgggcagg ctgggggtga atcagcgccc
     2341 cgatcatggg aaacatgatc tgcgtgccgg cgtccatcag cgcgttcccg tcgctcttgc
     2401 cgttgacaat gaaagtcacc ccgatattgg ttgaaacggc tacgctcgat tcctgaccgt
     2461 ccgcttccca ttcgatcgag cggcgcgcgg cgttcgacca cgccttgagg gcgttcatcg
     2521 tcatgtcagg catctgcgcg cggccgacgc cgatatggct gtgacgccag accgccgtgg
     2581 gaccggtcgc cagcaggcac agcgccgcga tcagcactgc aagcagggaa gacgcagtct
     2641 gaaccgcctg acggccagtg cgcaggccga taccaagcga cgccagcgag agacctgcca
     2701 ggagggccac cgtcagcttc caggcgcccg gggcggagag cagcggcaag aggccgaaac
     2761 cgcccgccaa ggaacccgcc atcgccccca gcgtgttgaa tgccgtcgcc tggccgacct
     2821 gcttgccaac gtccttccag ccgcggccta agagcgagat caacaacgga tattgcaacc
     2881 cgctgatgag ggcggcggga aagatgacga tccctgcgac gatgacccac cccgaggctt
     2941 gaccggcaaa gccgaagtat tcgagtccac ggagcacggc tgccagaatt gccagtcggt
     3001 cgcccgccgc gaatggcagg ccgatggcga gtgcttcccc ggcgcaagtc aaagcaaaat
     3061 cgcgcagcgt cggcaggcgc gcacgataaa gaagcgggta cagggctccg ccgataccga
     3121 tgcccgccag ggccaccgcc aggatgatgc caaacgtaaa ggttgtgccg ccgagaatcg
     3181 gcccgagcag gcgataccag acgagttcca tcagcagaaa cgcaaatccg accagtcccg
     3241 ctgcagcata gatcagccag ctcggagctt cgagcgggcc ctccatcaga tccgactgct
     3301 tcgcgggctc atcggcatcg ggtcggtgcc tgccgccggt cctgcgtttc gccgaggccg
     3361 ccggcgccgc cgaccactgc ccggcaagcc gccatgccag cgcggcattc acgatattgg
     3421 ccgccgcagc ggcccacaga gtttcgcgat tgcccagcat ctccagcaag acgaaggtgc
     3481 tggccaaggc gccaaccacg gccccgatcg tgttcaggcc atagagccag ccgacgctca
     3541 cgcgggcgcg gtcggcggcg ctggtggcgg ctcgcgcggc ggcggggagc gtgccgccca
     3601 tcaggaatgt cggaacgccg atgacgagcg tagaaaggag caggcgcacc atcgacgccc
     3661 cggagatgcc gagcgattcc tgcccgccca ggctgaagta caggctgcgg acgcattcca
     3721 acagaaaagg gctggcggcc gcggcggcgc tgatgcccag ttcaaacagg gcatagacct
     3781 tgagcgggct ggctgcgcga tcgacgcggc ggccgagcac cgcattcccc aggccgaggc
     3841 cacccatgaa gatcgccagg accgccgcgc tggcgggcgt gctcgcgccg aagatcaagc
     3901 ggaactcacg gagccaggcc gtctgaaaaa tgagcgcgca gagacccgag acgaag
//
LOCUS       DASUGB010000335         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1149362, whole genome shotgun sequence.
ACCESSION   DASUGB010000335 DASUGB010000000
VERSION     DASUGB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149362"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFV87_06445"
     CDS             <1..569
                     /locus_tag="VFV87_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103430.1"
                     /translation="ATPARGTLFGNRGGRLHDSQQRLTTRRWVNRAWICCKLAFNNRH
                     RKVWGNSYTELFFLDEVTAFAAGHRPCFECRRKDAERFALLFSGKKQRASAATMDKTL
                     HAERLDGKTKRSHRGRLDTLPDGAMISLDGQAFAVRGNRLLRWGPGGYSQSQSRPRGV
                     VVEVLTPPSILTVLRRGYGPLWHPSVTR"
     gene            592..1794
                     /locus_tag="VFV87_06450"
     CDS             592..1794
                     /locus_tag="VFV87_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5103431.1"
                     /translation="MSTPAALRRRRDYDGPALFSYGFRPFFLAAGLWAVVGILLWIPQ
                     YMGKLSVPTHLSPLDWHVHEMLYGYVAAAIAGFLLTAIPNWTGRLPVNGWPLAGLAAL
                     WLAGRLALLISAQLGGLVAAIVDMAFLVTLAVVAAREIIAGENWRNLRVLVILVVLVL
                     GNIVFHAEVLMTGNAAYGTRIAIAAVILMISLIGGRIVPSFTNNWLNRNNPGRLPVPF
                     SQFDMVALVISVFALVSWIITPMHWLSGALLIVAGCLQAVRLARWAGDRTVADRLVLI
                     LHIGYAFVPLGFVMVGLAAFTVEVPATAGIHAWTAGAVGLMTVAVMTRATLGHTGQPL
                     QAGRATQAIYALLLAAVLVRIVAAFMGSVDLLEFAGLCWTAAFILFVLLYGPLLAMRD
                     PGWSETRR"
     gene            complement(1796..>2000)
                     /locus_tag="VFV87_06455"
     CDS             complement(1796..>2000)
                     /locus_tag="VFV87_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103432.1"
                     /translation="SAVGLYVTSFYLGGSFGAAVGAIAWTLGGWPACAALVATMLAVL
                     AAIVIFGWSGTARQEKPSPIEPP"
BASE COUNT          302 a          663 c          638 g          397 t
ORIGIN      
        1 tcgcgacgcc cgcacgcggg acgctgtttg gaaaccgggg cgggcggctg catgactcgc
       61 agcagaggtt gacgacgcgc cgctgggtca accgcgcttg gatctgttgc aagctcgcgt
      121 tcaacaaccg tcaccggaag gtctggggca acagctacac cgagctgttt ttcctcgatg
      181 aagtgacggc cttcgccgcg ggtcacaggc cctgtttcga atgccggcgc aaggatgccg
      241 agcggtttgc tcttctattc tccggcaaga agcagcgcgc atcggctgca acgatggaca
      301 aaactcttca tgccgaacgg cttgatggaa agaccaagcg gagccatcgc ggcaggctcg
      361 atactcttcc cgacggggcg atgatttcgc tggacgggca ggcatttgca gtgcggggga
      421 accgcctgtt gcgctggggg cctggcggct actcgcaatc gcaatctcgc ccgcgaggtg
      481 tcgtggtcga ggtgctgacg ccgccgtcaa ttctcaccgt actcaggcgc ggctatggtc
      541 cgctctggca tccgagcgta acgcgctaga ctggacgcag aaaggcccag aatgtcgacg
      601 cctgccgccc tgcgacgccg ccgcgactat gacggccccg cactgttttc ctatggcttc
      661 cgcccgttct tcctggcggc gggtctgtgg gcggtcgtcg gcattcttct ctggattccc
      721 caatacatgg gcaagctgag cgtgcccacg catctaagtc cgctcgattg gcacgttcac
      781 gaaatgctct atggctacgt ggcagcggcc atcgccggtt tcctgctgac ggcgattccg
      841 aactggaccg gccggttgcc ggtcaacggc tggccgcttg cgggcctcgc cgcgctctgg
      901 ctcgccggac gccttgcgct cctgatatcg gcgcagctcg gcggccttgt cgcggcaatc
      961 gtcgacatgg cgttccttgt cacgcttgca gtggtcgccg cgcgcgagat catcgccggc
     1021 gaaaactggc gaaatctccg ggtgctggtg attctcgtcg tgctcgtcct cggcaatatt
     1081 gtctttcatg ccgaagtcct gatgacgggc aatgccgcct acggcacccg cattgccatc
     1141 gctgcggtca tcctgatgat ttcgctgatc ggcggacgga tcgtgccgag cttcaccaac
     1201 aactggctca accgcaacaa tcccggccgg ttgccggtcc cgttctcgca gttcgacatg
     1261 gtggcgctcg tcatcagcgt gtttgcgctt gtcagctgga tcatcacgcc catgcactgg
     1321 ctttccggcg cgctgctgat cgttgccgga tgcctgcagg cggtgcggct cgcgcgctgg
     1381 gccggcgacc gcacggtggc cgatcgcctc gtcctgatac tacacatcgg ctacgccttc
     1441 gtgccgctcg gtttcgtcat ggtcggcttg gcggcattca ccgtggaagt gccggcgacg
     1501 gcgggaatcc atgcctggac cgccggtgcc gtcggcctga tgacggtcgc ggtcatgacg
     1561 cgggcgaccc tcgggcacac cggtcagcct ctgcaggccg gacgcgcgac gcaggcgatc
     1621 tatgctttgc tgctcgccgc cgtgctcgtt cgcatcgtgg cggccttcat gggctcggtc
     1681 gatctgctcg aattcgcggg cctttgctgg accgcggctt tcatcctgtt cgtcctgctt
     1741 tacgggccgc tgctcgcgat gcgcgatccc ggctggagcg aaacacgccg ctagctcacg
     1801 gcggctcgat cgggctcggc ttttcctggc gggcagtgcc gctccacccg aaaatgacga
     1861 tcgcggccaa aaccgccagc atcgtcgcaa ccagcgcagc gcaggccggc cagccgccga
     1921 gcgtccaggc gattgcgcct accgccgccc cgaagctgcc gccgagatag aatgaggtca
     1981 cgtagagccc gaccgccgac
//
LOCUS       DASUGB010000336         5191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_455428, whole genome shotgun sequence.
ACCESSION   DASUGB010000336 DASUGB010000000
VERSION     DASUGB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455428"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..823)
                     /locus_tag="VFV87_06460"
     CDS             complement(2..823)
                     /locus_tag="VFV87_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5103433.1"
                     /translation="MAVRRKIFAPREYGLIARLAAVLLLLFAAPVAFAQEPTEAEQAV
                     ENGGEALQESGRYPWYDAREDGVRTIPLANSGDADSKNRDSKWTSQPKTGRPWTFGGF
                     GFFGTLLNGLGVALLILFLGLIAYLIAKAFLKDETSDGVTRKFVETSRDVDRVEALPF
                     KIRKPTGDFLSEARRLYEAGEYSEAVIYYFSYQLVELDRHHVLRLAKGKTNRQYLREV
                     RQRPVVRSILEATMITFEDSFFGRKAISRQQFENCWNRLSDFQGELARLERAAAA"
     gene            complement(1068..2009)
                     /locus_tag="VFV87_06465"
     CDS             complement(1068..2009)
                     /locus_tag="VFV87_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103434.1"
                     /translation="MQLDNTRIAIRERDLLETLDLALHVTRQFAGPWLICSLLGIVPL
                     ALVNYALVGWMIPWGWDEEGLPWRYLWSMSILVYLEAPLASAFVVAYLGPAVFLETKT
                     IRQVVSDVFHYALQISLCQFLLRGVLAAWLLYFLVERYQFSGPVEGFLLPCLALYATG
                     MRAFRPYINEIILLERNPLRSNNPNVITVNKRSSHIHGPYSGDLFIRWLGLALIALLL
                     IFMVLATAIVVQGVLIGRWPFVFDTTRGPAPWDWIVWQLNWFEAQVAFPLCIWLIVAF
                     MSVVRFLSYLDLRIRHEGWEVELLMRAEAMRLAARME"
     gene            complement(2066..3226)
                     /locus_tag="VFV87_06470"
     CDS             complement(2066..3226)
                     /locus_tag="VFV87_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX5103435.1"
                     /translation="MKVVELLERRRKNWHELEMMCDGMQGGARPYHPGAVAGRVPGVM
                     AGTAAVNAVQMVQANTTVLPAEQISRFGALYRAACADLALAYAYQLPQNTVAYLHRLV
                     GRAHNQLYRSRRFEFRKWGEMLLFDVPKMIFNDRCVQAMFVVFWGLFILCAFLGYSKT
                     AWPDFAEQVLGPEFIQQLEEMYKNPIDGSLRGGDENMMMAGMYIQHNTSIGLQCFAWG
                     LLVVPGLLMTMSNAIQLGTAFGYMARPDVPEGENFFHFVTAHGPFELTAIVLSAGAGL
                     RLGLSWMITGGLSRGAALIQAGRQVIPVVGAAAAMFFMAALIEGFLSPSGAPYWLKVF
                     VAILSSSLLAFYFVVLGFPRGITMPTHGRHPLDDAPEAWRKEPSTEETRTKE"
     gene            complement(3343..4299)
                     /locus_tag="VFV87_06475"
     CDS             complement(3343..4299)
                     /locus_tag="VFV87_06475"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5103436.1"
                     /translation="MAWKDQPLDSTIDVVTPENIAFEYRLAGPFRRLPALLFDIFVCR
                     AAQMALFCGIGMSVGMVSQGLAVFLIFLSIFIIDWFYGILFETFLNGQTLGKWVFGLR
                     VLTDRGEPINGMQATLRNLLRSADLLMGIFGTIGITAMAMNRRYQRLGDLVAGTIVVV
                     EERRWLTGVAKLDDPRAIQLAAYLPPNFVVSRSLSKALSTYVERRRFFTPPRRREVAR
                     HLAEPLLERFGLPPDTSYDLLLCSLYYRTFIADRSQDERLLAEAQAAFAAANPFAAFA
                     GGRGMSASPYPGMPNSAGITFVNPPAPLPQEQMVGTGFGAGR"
     gene            4486..>5191
                     /locus_tag="VFV87_06480"
     CDS             4486..>5191
                     /locus_tag="VFV87_06480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5103437.1"
                     /translation="MSNSALSNKTALITGGGSGIGLGTALALAREGCRVAIAGRRLEV
                     LKQAAATWTGKPPILCQAVDVADRGSVEQLFGWAAKELGPIDILVNAAGVNIKNRLMS
                     NMEPPDWDRVLAINATGVYNCMYFVLPQMRQRRDGLIVNISSISGKRAAALGGVAYNA
                     SKFAMAALGTSVSNEEVPNNIRLTNIYPGEVDTPILEHRPQPVSAERRAKMLLPEDVG
                     DLVVAIARLPPRAHVPE"
BASE COUNT         1080 a         1601 c         1613 g          897 t
ORIGIN      
        1 ctcatgcggc ggcggcgcgc tccaggcggg ccagctcgcc ttgaaaatcg ctcagtcggt
       61 tccagcagtt ctcgaactgc tggcggctga tggctttgcg tccgaaaaac gagtcctcaa
      121 aggtgatcat cgtcgcttcc aggatcgacc gcacgaccgg ccgctggcgg acctcgcgga
      181 ggtattgccg gttggtcttc cccttcgcca gccgcaaaac gtggtgccgg tcgagctcga
      241 caagctggta gctgaagtaa taaatgaccg cctcggaata ctcgcccgct tcgtacagcc
      301 ggcgggcctc gctgaggaag tcgccggtcg gcttgcggat tttgaacggc agagcctcga
      361 cgcgatcgac gtcgcgcgag gtctcgacga acttgcgggt cacgccgtcg ctcgtctcgt
      421 ccttgaggaa cgctttggca atcaagtaag cgatcaaccc cagaaacaag atcagcagtg
      481 cgacgcccag cccgttgagc aaggtgccga aaaagccgaa gcccccaaag gtccacggcc
      541 tgcccgtctt cggctggctg gtccatttgg agtcgcgatt cttggagtcg gcatcgcccg
      601 aattggccag cgggatcgtc cgcacaccgt cctcgcgggc gtcgtaccag ggataccggc
      661 cgctctcctg cagcgcctcg ccaccgtttt ccaccgcctg ctccgcctcg gtcggctctt
      721 gggcaaaggc gaccggcgcc gcgaaaagca aaagcaaaac ggcggccagc ctggcaatca
      781 ggccgtattc acgcggggcg aatatttttc tgcggacggc catcggatcg taagtcgttc
      841 ctgtttatgg agttatggaa aaagtgcagc ctatgcgttt attcatttta ttcacgcgat
      901 gcataaacgc ccgccgtcac ccggctggcg tcgataggcg gttcgccacc gtattaaacg
      961 cacacaggtg gacaagtctg gtccatttca gtcaagtgaa aaaaagattt ttctgtcccg
     1021 caaagaactc cctccagctt tgaattcgtt cactctgcgc cctgattcta ttccatccgc
     1081 gccgccagcc gcatcgcttc ggcccgcatg agcagttcga cttcccaccc ttcgtggcgg
     1141 atgcggagat cgagatagct gaggaagcgg acgaccgaca tgaaggcgac aatcaaccag
     1201 atgcacaagg gaaacgcgac ctgcgcctcg aaccaattga gttgccacac gatccaatcc
     1261 cagggagctg gcccgcgcgt cgtgtcaaaa acaaacggcc atctgccgat gaggacccct
     1321 tgcacgacaa tggcggttgc cagcaccatg aaaatcagca gcaaggcgat cagcgccaac
     1381 cccaaccagc gtatgaacaa gtctcccgag taagggccgt ggatgtggga gctgcgcttg
     1441 ttgactgtga tgacgttggg gttgtttgaa cgaagcgggt ttctctcgag gagaatgatt
     1501 tcgttaatat atggccggaa ggcccgcatt cccgtggcat acaaggccag gcacggcagc
     1561 agaaagcctt ctacggggcc gctgaattga taccgttcga caaggaagta taacaaccac
     1621 gccgccagaa cgccgcgcag cagaaattgg caaagggaaa tctgtaatgc gtagtgaaac
     1681 acgtcgctga ccacctggcg gatggtcttc gtctccagaa agacagccgg ccccaggtac
     1741 gcgaccacga aggccgaggc cagaggcgct tccaggtaca cgaggatgct catgctccac
     1801 aaataacgcc acggaagccc ttcttcgtcc caaccccaag ggatcatcca gcccacgagc
     1861 gcgtagttga ccagcgccag cggcacgatg cccaagagcg agcagatcag ccacggcccc
     1921 gcgaactgcc gcgtgacgtg cagcgccaga tcgagcgttt ccaagaggtc acgctcgcgg
     1981 atcgcaatgc gggtgttgtc taattgcatg caataaattc caagaaacaa gaaccgaaga
     2041 accaagttgc ggagtggctt gggccctatt ccttggttct tgtctcttcg gtacttggtt
     2101 ctttccgcca cgcctccggc gcgtcatcca gtggatgccg tccatgagtt ggcatcgtga
     2161 ttccgcgcgg gaagccgagc acgacgaaat aaaacgccag caggctgctg gagaggatcg
     2221 ccacgaaaac cttcagccag tacggcgcgc cactgggcga aaggaagcct tcgatcagcg
     2281 cggccatgaa gaacatcgcg gcggctgccc cgacgaccgg aatgacttgc cgcccggcct
     2341 ggatcagggc cgcgccgcgg ctgaggccgc cggtgatcat ccacgagaga cccagccgca
     2401 agcccgcgcc agccgaaagc acgatggcgg tcagctcgaa cggcccgtgg gcggtgacga
     2461 agtggaagaa attctcccct tcgggaacgt cgggccgggc catgtagccg aacgccgtgc
     2521 cgagctgaat ggcgttggac atcgtcatga gcaggcccgg cacgacgagc agtccccagg
     2581 caaagcactg caaaccgatg ctcgtattgt gctggatata catgccggcc atcatcatgt
     2641 tctcgtcgcc gccgcggagc gagccgtcga tcgggttctt gtacatctcc tcgagctgct
     2701 ggatgaattc cggccccagc acctgctcgg cgaagtccgg ccaggcggtc ttcgaatagc
     2761 cgagaaacgc acagaggatg aagaggcccc agaaaacgac gaacatcgcc tgcacgcagc
     2821 ggtcgttgaa gatcatcttg gggacgtcga acagcagcat ctcgccccat ttgcggaact
     2881 cgaatcgccg gctgcggtag agctggttgt gggcccggcc gaccagccgg tgcaaatagg
     2941 cgacggtgtt ctgcggaagc tgataggcgt aagccagggc caggtccgcg caggcggcgc
     3001 gatagagtgc cccgaaacga gagatctgtt cggcgggcag gaccgtagta tttgcctgca
     3061 ccatttggac ggcatttacc gcagcagttc ctgccattac tccgggcact ctgcctgcga
     3121 cggcaccggg gtggtagggc ctagcgcccc cttgcatccc gtcacacatc atctccagct
     3181 cgtgccagtt cttgcggcgg cgttcgagga gttcgacgac tttcatggtt gcgtggggta
     3241 ggcgtctcgc ctgcccgtcg tagccgcgtt cgccagaacg cgggtctgcg gaaatctgca
     3301 ctcgggttgg cccgcactct ggcgagtgcg gctacgagtt ggtcatcgcc ctgctccgaa
     3361 ccccgtgccg accatctgct cttgcggcag cggggcggga gggttaacga acgtgatgcc
     3421 ggcggaattc ggcatcccgg gatacggact ggccgacatt cctcggccgc cggcgaaggc
     3481 ggcaaagggg ttggcggcgg caaacgcggc ctgggcctcg gcgagcaggc gctcgtcctg
     3541 gctgcggtcc gcgatgaaag tgcggtaata gagcgaacat agcaggaggt cgtagctcgt
     3601 atcggggggc aagccgaacc gctccaaaag cggctcggcc aggtggcggg cgacttcgcg
     3661 gcggcgcggt ggcgtaaaga accgccgccg ctcgacatac gtcgaaaggg ccttggacag
     3721 cgagcgggag acaacgaagt tcggcggcag ataagccgcg agctggatgg cccgcgggtc
     3781 gtcgagcttc gccacgcccg tcagccagcg gcgctcctca accacgacga tcgtccccgc
     3841 gaccagatcg cccaggcgtt gataccggcg gttcatcgcc atcgcggtaa tgccgatcgt
     3901 tccaaagatg cccatcagca aatcggcgga ccgcagcaaa ttccgcagcg tggcctgcat
     3961 gccgttgatc ggctcgccgc gatcggtgag cacccgcagg ccaaagaccc atttgccgag
     4021 cgtctggccg ttgaggaacg tctcgaacag gatgccgtag aaccagtcga tgatgaagat
     4081 gctgaggaag atgagaaaca cagccagccc ctggctgacc atcccgaccg acatgccgat
     4141 gccgcagaag agcgccattt gcgcagcccg gcagacgaag atgtcgaaca acagcgccgg
     4201 caagcggcgg aacggcccag ccaggcggta ctcgaacgcg atgttctccg gagtgacgac
     4261 gtcgatcgtc gaatccagcg gctgatcttt ccaggccatg cgcggaacgt cacgtgcggg
     4321 aacaaggcgg cgcccgccgc ctcagcgggc agcgtgcatt attcgctggc gctgggcggc
     4381 cagcaagcgt gcggatcgtg acaagtcttg ttcaaacaga tgttgacagc gggtaaactc
     4441 agcagcgtac gtccaagttc tattcgctga actccatcaa ccatcatgag caactccgca
     4501 ctttcgaata aaaccgccct catcaccggc ggcggcagtg gcattggcct tggaacggct
     4561 ttggcgctgg cccgcgaggg ctgccgtgtg gcgattgccg ggcggcggct ggaagtcttg
     4621 aaacaggccg ccgcgacctg gaccggcaaa ccgccgatcc tctgccaggc cgtcgatgtg
     4681 gccgatcgcg gcagcgtcga acagcttttc ggctgggcgg cgaaggagct gggaccgatc
     4741 gacatcctgg tcaacgcggc gggcgtgaac atcaagaacc gcctgatgtc gaacatggag
     4801 ccccccgact gggaccgcgt tttggcaatc aatgcgaccg gcgtctacaa ctgcatgtac
     4861 ttcgtcctgc cgcagatgcg ccagcggagg gacgggctga tcgtcaacat ctcgtcgatt
     4921 tccggcaagc gggcggcggc cttgggggga gtggcctata acgccagcaa attcgcgatg
     4981 gccgctttgg gaacgagcgt ttccaacgaa gaagtgccca acaacatccg cctgaccaat
     5041 atctatccgg gcgaagtcga tacgcccatc ctcgagcacc ggccgcagcc cgtcagtgcc
     5101 gagcggcggg cgaaaatgct gctgccggag gacgtcggcg acctggtggt cgccattgcc
     5161 aggttgccgc cacgagccca cgtgccggag c
//
LOCUS       DASUGB010000337         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1567607, whole genome shotgun sequence.
ACCESSION   DASUGB010000337 DASUGB010000000
VERSION     DASUGB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567607"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..529
                     /locus_tag="VFV87_06485"
     CDS             128..529
                     /locus_tag="VFV87_06485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5103438.1"
                     /translation="MKYALVKAALPHLRKQRGGAIVTVSSSAGVRGVPGAIAYATAKG
                     ALPQFTRSLARDLADDNIRVNCVAPGVIRTRFHADMTPERKELNLKDRIPLHREGTPE
                     QVAEAVALLITNDYITGETIVIDGGLTSRIA"
     gene            599..1150
                     /locus_tag="VFV87_06490"
     CDS             599..1150
                     /locus_tag="VFV87_06490"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5103439.1"
                     /translation="MSGANSLLTAAEFSRMADPGHPQELVRGIVVNMPPPKPRHGKIC
                     GRIIAHLVAYCDASDLGHVLGNDSGVVTEQDPDTVRGPDVTFIGYAKLPRDADLDDYV
                     EIAPDAVFEVRSPSDGWSKILRMVAEYLQLGVLAVYVFDSEMEQVHCYYPDKPEQILT
                     AADELAGVGPLAGFRVPVAKFFA"
     gene            1238..>2698
                     /gene="dnaE"
                     /locus_tag="VFV87_06495"
     CDS             1238..>2698
                     /gene="dnaE"
                     /locus_tag="VFV87_06495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5103440.1"
                     /translation="MSAGFSRPFVHLHCHSHYSLLDGASEIGKLTKRAKELGMNALAL
                     TDHGNLHGALEFYRAAKDAGLNPIVGYEAYIAPGSRFEKKDAANSKEASYHLTLLAQN
                     RTGFKNLVKLASAASLEGFYYKPRIDKELLAAHHEGIVCLSGCVSSELSMTLLKGGAV
                     ESDFREAREIAEWFRSIFGERYFIEIMNNGVEVQRIQLEGAVDLAMRLGIPLVATSDA
                     HYINREDAVAQDALLCINTGKFRTDTNRMRMENDSFYLRSPEEMYAHFPNLEEAVARS
                     QQIADTISLDLELGKRHFPVFQLPGEKSAEDYLRELCLQGLKERYAGDEEMCKGGELS
                     EVVMQRLERELSVINQLGFPNYFLICWDFVRHAREQGVPATARGSGVGALVCYALYLS
                     HVCPIRYDLLFERFLDLNRKEAPDIDIDFCKDRRGEIIRYVKEKYGEANVAQIGTFGT
                     LAAKAAITDVGRVLGWPVPRVNALKAMVPEQLGISLD"
BASE COUNT          517 a          847 c          831 g          503 t
ORIGIN      
        1 gggttccatc gtggaccccg cgggcgattc caccgcccca cgggatcggt ttgccatgtg
       61 tgacctcctt ggggtttcac tccatacggc aaacctccaa ctgaggttta gctagcaaaa
      121 acacgttttg aaatacgctc tcgtcaaagc ggcactgccg caccttcgca agcagcgcgg
      181 gggggcgatc gtcaccgtct cctcctcggc cggagtccga ggtgtgccag gcgcgatcgc
      241 ctacgccacg gccaaaggtg cgcttccgca gttcacgcgc agcctggccc gcgatctggc
      301 cgacgacaac atccgcgtga actgcgtcgc ccccggcgtc atccgcacgc gcttccacgc
      361 cgacatgacg cccgagcgca aggagctgaa tctcaaggat cgcatcccgc tgcaccgcga
      421 aggcacgccc gagcaggtcg ccgaagcggt cgcgctgctg atcaccaatg actacatcac
      481 gggcgaaacc atcgtcatcg acggcggcct gacgagccgg atcgcatgag tttggcgagc
      541 gatgcttgtc gtgctagaat gattgcgcca acggtctccg gaggtgtcaa gccaatcgat
      601 gtcaggtgca aattcactac tgaccgctgc tgaattttcc cggatggccg atcccggcca
      661 tccgcaggaa ctagtgcgcg gaattgttgt taatatgccg ccgccgaaac cgcgccacgg
      721 aaagatctgt ggaagaatca tcgcccatct cgtcgcgtat tgtgatgcga gcgaccttgg
      781 ccatgttctc ggaaacgaca gcggtgttgt tacagaacaa gacccagaca cggtgcgcgg
      841 acccgacgtg acgtttatcg gctatgccaa gctgccgcgc gatgccgacc tggacgatta
      901 tgtcgagatt gcgccggacg ctgttttcga ggtccgctcg cccagtgacg ggtggagcaa
      961 gattctgcga atggtagctg agtatttgca actcggtgtg ctagccgttt acgtgttcga
     1021 ctcggagatg gagcaggttc actgctacta tcccgacaag ccggagcaaa tcctgaccgc
     1081 tgccgacgag cttgccggcg ttggcccctt ggccggattt cgcgttcctg tcgcaaagtt
     1141 ctttgcgtag cggcgccccg ctggcgcggc gcgcggccca gtcgcttcgc tataatcacg
     1201 ggtttgcagc aggattctcg cagcagggat cgttggcatg tcggctggtt tttcgcgccc
     1261 cttcgtccat ctccattgcc attcgcatta cagcctcctg gatggggcca gcgagatcgg
     1321 caagctcacc aagcgggcca aagagttggg gatgaacgcc ctggcgctga ccgaccacgg
     1381 caacctgcac ggggcgctgg agttttatcg cgcggcgaag gacgccggcc tcaaccccat
     1441 cgtcggctac gaggcgtata tcgcccccgg cagccggttt gagaaaaagg acgccgccaa
     1501 cagcaaggaa gccagctacc acttgacgct gctggcccag aatcgcaccg gcttcaagaa
     1561 cctggtcaag ctcgcatcgg cagcgtcgct ggaaggcttc tactacaagc cgaggatcga
     1621 caaggaactg ctggcggccc atcacgaggg gatcgtctgc ctgtcaggct gcgtctcgag
     1681 cgagctgagc atgacgctgc tcaagggcgg agcggtggaa tcggattttc gcgaggcccg
     1741 cgagatcgcc gagtggttcc gctcgatttt cggtgagcgg tactttatcg agatcatgaa
     1801 caacggcgtc gaggtacagc ggatccagct cgaaggggcc gtcgatctgg cgatgcggct
     1861 aggcattccg ctggtggcaa cgagcgacgc ccactacatc aaccgcgagg acgccgtcgc
     1921 ccaggacgcc ctgctctgca tcaacacggg caagttccgc accgatacca accgcatgcg
     1981 gatggagaac gattcgttct atctccgctc gccggaggag atgtacgccc atttccccaa
     2041 cctggaagaa gccgtcgccc gcagccagca gatcgcggat acgatcagcc tcgacttgga
     2101 gctcggcaag cggcactttc cggttttcca gcttccaggg gagaaatcgg ccgaagacta
     2161 ccttcgcgag ctgtgtctgc agggcctcaa ggagcgctac gcgggcgatg aggagatgtg
     2221 caagggcggc gagctctccg aggtcgtcat gcagcgcctg gagcgcgagc tgtcggtcat
     2281 caaccagctc ggctttccga attacttcct gatctgctgg gatttcgtgc ggcacgcgcg
     2341 ggagcaaggc gtgccggcga ccgcccgcgg cagcggcgtc ggggcgctcg tctgttatgc
     2401 cctctatttg agccacgtct gcccgattcg gtacgacctg ctcttcgagc ggttcctgga
     2461 cctgaaccgc aaggaagcgc ccgatatcga tatcgacttc tgcaaagacc gccgcggcga
     2521 gatcatccgc tacgtcaagg aaaagtacgg cgaggccaac gtcgcccaga tcggcacctt
     2581 cggcacgctc gcggccaagg cggcgatcac cgatgtcggc cgcgtcctcg gctggccggt
     2641 gccgcgggtc aacgccctga aggcgatggt tcccgagcag ctcggcattt cgctcgac
//
LOCUS       DASUGB010000338         4778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1705796, whole genome shotgun sequence.
ACCESSION   DASUGB010000338 DASUGB010000000
VERSION     DASUGB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705796"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VFV87_06500"
     CDS             complement(<1..1701)
                     /locus_tag="VFV87_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEX5103441.1"
                     /translation="MLLADFLNTLVWWQWGLLALVPPAIIALYFLKLRREPLAVPSTY
                     LWSRAIEDLHVNSLWQRLRQSLLLFLQLLLIALLAFTLLRPGWEGSKLEGERFIFLID
                     TSASMAATDVGPSRLDEAKRQVIGMIEQMRPENAAMVISFSNVARVEQPFTNNRRTLR
                     TKVELIEQTNRPSDLSEALRAASGLANPGQSGDPNNPVDNPTAEAMPATLYILSDGGF
                     SSVPNFRLGNLTPIYLKTAGEGQENVAIVAFSTEVNPEKPGRVQAFGRLENYADAERT
                     VEASLFLNDTLLDAKKVTLPIRDPQNKLPGASGVNFEMQEIESGILKLEIQPKDQMLA
                     DNTAWAVVNLPRPAKVLLITPGNDALELALRTDEAQKVADVSVAEPKLLESKAYEDQA
                     AEGAYDLIIYDQCAPKKMPACNTLFIGRVPPSEEPATPAAPPAEGAAAAPKVQGWQAG
                     QRQGAPVIIDTDQVHPLTQLMQMGNVLVIEGTPIKGPPGTVTLVDSDIGALYAVGPRG
                     GYEDAVLGFDIISYTAEGTREANTNWPRRRSFPVFVLNAVKYLGGVRSSLASPNIQPG
                     Q"
     gene            complement(1745..2500)
                     /locus_tag="VFV87_06505"
     CDS             complement(1745..2500)
                     /locus_tag="VFV87_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX5103442.1"
                     /translation="MPIHNWKKVAAGTFHDFHQGWSIAIRSRLNAGLLPQDYYALIEQ
                     PSGDIEPDVLTLRHSNGNLRESDDTGTTLTIETAPPAVQFSAFLEEEWYLYKKNRIGI
                     HHASDDRMVAMIELVSPGNKSSRHRFEKFTDKVLACLEAGIHLLLIDLFPPTRRDPQG
                     LHGAIWSELGDDSFDLPADKRLTLVAYASDRPIRAYVEPLSVGDPLTPMPLFLTPSHY
                     VVVPLEETYATALQGWPPHLVEKLNRTLPRASS"
     gene            complement(2531..3427)
                     /locus_tag="VFV87_06510"
     CDS             complement(2531..3427)
                     /locus_tag="VFV87_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5103443.1"
                     /translation="MPETATTPQLLSPQLLAQLERLELVTRKVFRGRMKGERRSKRKG
                     QSVEFADFRQYVPGDDLRFIDWNLFARMERLYLKLFLEEEDLHFYALIDASDSMEFGE
                     PTKLQFAKQLAASLGFIGLCRADRVKIETLATSSRKPGPTLRGRSSLWRMMEYLEGIE
                     PGENVSLAEGVKNFCLRNTGKGILVLISDLMDKEGYEKALRFLVAQQMDVYLLHVLCP
                     EELDPEVKGDLKLIDCEDDDIAEITVSRPLLDKYKRTLAAFVDGAREFCTRRGMTYLM
                     TSTQTPVETLVANYLRKRGLVR"
     gene            complement(3484..4026)
                     /locus_tag="VFV87_06515"
     CDS             complement(3484..4026)
                     /locus_tag="VFV87_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103444.1"
                     /translation="MAKSIPAPPIRTTPKGRYSLRTLFLISGLIALPFLLMANALHQA
                     RPDDSIASPLYLALGVGGVVLAAAIGSALGSRAGMLTCAGLAAVGWIALVLVCSEFSQ
                     KLASVLPVHVLAAAVTVVVLAFVVRTPKESSEEGPHGMLVKLLKVKRDVQDAGQAQGS
                     LENPDESDSNAAKDKPGPAG"
     gene            complement(4213..4656)
                     /gene="tnpA"
                     /locus_tag="VFV87_06520"
     CDS             complement(4213..4656)
                     /gene="tnpA"
                     /locus_tag="VFV87_06520"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX5103445.1"
                     /translation="MASHVYHEIYLHITWHTKDDSRLLTPHLEPAAHEVLRDRCHQTK
                     GVYFHGINGTTTHIHLAVNIEPHVTISELVGELKGFSSHEINKQERMKRLQWQRGFGV
                     VSFGKMNLPWVQNYIARQKEHHAAGTLEDRLERVSFDDDGSPLDA"
BASE COUNT          880 a         1489 c         1447 g          962 t
ORIGIN      
        1 ctggccgggt tgaatgttcg ggctggcgag gctggagcgc acgccgccca ggtacttcac
       61 ggcgttgagg acgaagacgg ggaagctgcg acgccgcggc cagttggtgt ttgcttcgcg
      121 agtgccttcg gccgtatagg agatgatatc gaagcccagc acagcgtcct catagccgcc
      181 acgcgggccg acggcgtaca gggcgccaat atcggaatcg acgagcgtca cggtccccgg
      241 cggacctttg atcggcgtcc cctcaatcac cagcacgttg cccatctgca tgagctgcgt
      301 cagcgggtgg acctggtcgg tgtcgataat caccggcgct ccctgcctct gcccggcctg
      361 ccaaccctgg accttcggcg ctgcggccgc gccttccgcc ggcggcgcgg ccggcgtcgc
      421 gggctcttcc gacgggggga cgcgcccgat gaacagcgta ttgcaagcgg gcatcttctt
      481 cggggcacac tggtcgtaga tgatcaggtc gtacgcccct tcagccgcct ggtcttcgta
      541 cgccttgctc tccagcagct tgggctcagc gaccgaaacg tcggcgacct tctgagcctc
      601 gtccgtccgc agggccagtt ccagggcgtc gttgccgggc gtaatcagca ggactttggc
      661 aggccgcggc aaattgacca cggcccacgc ggtattgtcg gccagcattt ggtccttcgg
      721 ctgaatttcc agcttcagga tgccgctttc gatctcctgc atttcgaagt tcacgccgct
      781 ggcccccggc agcttgtttt gcgggtcgcg gatgggcagc gtcaccttct tggcgtcgag
      841 cagcgtgtcg ttgagaaaga gcgaggcctc cacggtcctt tcggcatccg cgtaattctc
      901 caagcggccg aaggcctgca cgcgtccggg cttttccggg ttcacttcgg tgctgaacgc
      961 gacgatggcg acgttctctt gcccttcacc cgccgtcttc agataaatcg gcgtcaggtt
     1021 ccccagccgg aagtttggca cggacgaaaa tccgccgtcg ctgaggatat acagcgtggc
     1081 gggcattgct tcggccgtcg gattatcaac tggattattc gggtcgccgc tttgaccggg
     1141 attggccagg ccggaggcgg cccgcagggc ctcgctcagg tcgctcggcc ggttggtctg
     1201 ctcgatcagc tcgactttcg tgcgcaaggt gcggcggttg ttcgtgaaag gctgctcgac
     1261 gcgagccacg ttggaaaagc tgatgaccat cgccgcgttt tcgggtcgca tctgctcgat
     1321 catcccgatc acttggcgct tggcctcgtc gaggcgcgag gggccgacgt cagtcgccgc
     1381 catgctggcc gaggtatcga tcaggaagat gaaccgctcc ccctccagct tcgacccctc
     1441 ccacccgggc cgcagcagtg tgaacgcgag cagggcaatc agcaaaagct gcaagaagag
     1501 cagcaggctc tgccgcaggc gctgccacag cgaattgacg tgcagatcct cgatcgcccg
     1561 gctccagagg tacgtgctgg gcaccgccag cggctcgcgg cggagcttca ggaaataaag
     1621 cgcaatgatc gccggcggga caagcgccaa caggccccat tgccaccaga cgagcgtgtt
     1681 gaggaagtcg gcgaggagca tggcggatgt tcgttgcgat aggtaaagag gatgccggac
     1741 ctactcaact gctagcccga ggcaaagtgc gatttagctt ttccaccaaa tgcggcggcc
     1801 aaccttgcaa tgccgtcgca tatgtctcct ccaagggcac gaccacataa tgggagggag
     1861 tcaagaacag tggcatagga gtcagcggat cgcccaccga tagcggctcg acgtatgccc
     1921 gaattggtcg atccgacgca tatgcgacca gggtcaggcg cttgtcggcc ggcaaatcaa
     1981 aactatcgtc tccgagctcg gaccagatcg cgccgtgcaa tccttgtgga tcacggcgcg
     2041 tgggtgggaa gaggtcgatc aaaagcaaat gaattcctgc ttccaaacac gctaatactt
     2101 tgtctgtgaa tttctcaaaa cggtgtcggc tggacttgtt gccgggcgaa accagttcga
     2161 tcatcgccac catccggtcg tccgacgcgt gatgaatccc gatccggttt ttcttgtaga
     2221 ggtaccattc ctcttcgagg aatgctgaaa actgaacggc gggcggcgcc gtttcgatgg
     2281 ttaaggttgt tcccgtgtcg tcgctttccc gcaggttgcc gttcgagtgt cgaagtgtca
     2341 ggacgtcggg ctcgatgtcc ccggatggct gttcgatcaa ggcgtagtag tcctgtggca
     2401 gcagacctgc attaagtctg ctgcgaatgg cgatcgacca gccctggtgg aaatcgtgga
     2461 acgtcccagc agcaaccttt ttccaattgt gaatgggcat gatcttcctc ctcgggtctc
     2521 accattcatc ctaccggacc agcccgcgtt tccgcagata attcgccacc agcgtctcca
     2581 caggcgtctg cgtgctcgtc atcaggtacg tcatcccgcg gcgcgtgcag aactcgcggg
     2641 ccccatccac gaaggccgcc agcgtccgct tgtatttatc caagagcggg cggctgacgg
     2701 tgatctcggc gatgtcgtcg tcctcgcagt cgatgagctt caggtccccc ttcacctctg
     2761 ggtcgagctc ctccgggcaa agcacgtgca gaagatagac gtccatctgc tgggcgacga
     2821 ggaaccggag ggccttctcg tacccctcct tgtccatcag gtcgctgatc agcaccagga
     2881 tccccttgcc cgtgtttcgc aggcagaaat tcttgacccc ttcggccagg ctgacgttct
     2941 cgcccggctc aattccttcc agatactcca tcattcgcca caggctcgag cggccgcgaa
     3001 gcgtcggccc cggcttgcgg ctggaggtgg cgagcgtctc gatcttcacg cggtcggccc
     3061 ggcacaggcc gatgaacccc agcgacgcgg cgagctgttt ggcgaattgc agcttcgtcg
     3121 gctcgccgaa ctccatgctg tcgctggcgt cgatcagggc atagaagtgc aggtcctctt
     3181 cttccaggaa cagcttgaga tacagccgtt ccatgcgggc gaagaggttc cagtcgatga
     3241 accgcaggtc gtcgccggga acatactggc gaaagtcggc aaactcgacg ctttgcccct
     3301 tccgtttgct ccgccgctcc cctttcatcc gcccgcgaaa gaccttccgc gtcacgagct
     3361 cgaggcgttc gagctgggcc aggagctggg gggagagaag ctggggagtc gtggcagttt
     3421 caggcatggc gggtcaatat cgaagttcga tagagagaga gtcggcagcc ggcagattct
     3481 actctatccg gctgggccgg gcttgtcctt ggccgcattt gagtccgact cgtcagggtt
     3541 ctcaagactt ccttgggcct gcccggcgtc ttgcacgtcg cgtttcacct tgagcagctt
     3601 cacgagcatg ccgtgcggcc cctcttcgga cgattccttc ggcgtacgga cgacaaaggc
     3661 cagcacgacc accgtgacgg cggcagcgag cacatgaacc ggcagcaccg aggcgagctt
     3721 ttggctgaac tcactgcaca ccaagacgag agcaatccag ccgacagcgg cgaggccggc
     3781 acaggtcagc attcccgccc ggcttcccag agcgctgccg atcgctgcgg cgagcacgac
     3841 gccccccacg cccagcgcca gatacagcgg cgaggcaatg ctgtcgtccg gtcgagcctg
     3901 gtgcagcgca ttcgccatca gcaaaaacgg cagagcgatc aggccgctga tcaagaacaa
     3961 ggtgcgaagg ctgtagcgtc ccttgggagt cgtgcgaatc ggcggtgctg gaatagattt
     4021 ggccatagct agggtccttc ggcgctgcgg ccatttcttc acatcgctcc cggcaggagc
     4081 gacacgccgc atgttccgca ttcccctcag ccggcttcag ccggctcccc cgtcagccac
     4141 cggcttcagc cggtgattcg cgatgcacga cccaaatcta ccagccggct tcagccggct
     4201 tcggtccagc cttcaggcat ccaacgggct gccgtcatcg tcgaatgaga cgcgctccag
     4261 ccggtcctcc agcgtgccag ccgcatgatg ctccttctgg cgggcgatgt aattctgcac
     4321 ccagggcaga ttcatcttac caaagctgac cacgccgaac ccgcgctgcc actggagccg
     4381 cttcattcgc tcctgcttgt tgatctcgtg cgacgagaag cccttcagct cgccgacaag
     4441 ctcgctaatc gtgacgtgcg gttcgatgtt gaccgccaaa tggatgtgcg tcgttgtacc
     4501 gttgatgccg tggaaataga cacccttggt ttggtggcag cgatctcgca agacttcatg
     4561 ggcagcgggt tccaggtgag gcgtcaagag ccgcgagtca tccttggtat gccacgtgat
     4621 gtgaaggtaa atctcgtgat agacgtggct ggccatgatg agatctccgg ttgctgccta
     4681 acagcccgtt gaaaaaatca attttcaacc agtgctaact gcgtgaatcc cgaagaaaac
     4741 agtgctagca aaccccactt ctgggacttt ttcaacag
//
LOCUS       DASUGB010000339         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_872868, whole genome shotgun sequence.
ACCESSION   DASUGB010000339 DASUGB010000000
VERSION     DASUGB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872868"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2326)
                     /locus_tag="VFV87_06525"
     CDS             complement(<1..2326)
                     /locus_tag="VFV87_06525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103446.1"
                     /translation="MAGVQVTFDQPVTENPGTQIGPYKLLQQIGEGGMGVVYMAEQKE
                     PVERRVALKIIKPGMDTRQVIARFEAERQALAIMDHVNIARVLDAGTTEQGRPYFVME
                     LVHGVPITKYCDDNHLTPRQRLELFVPVCQAIQHAHQKGIIHRDIKPSNVMVTLYDGQ
                     PVPKVIDFGVAKATEQKLTERTLFTQYGTMVGTLEYMSPEQAEMSALGVDTRSDIYSL
                     GVLLYELLTGSTPLTHKRMKEAAYAEILRMIKEEEPPKPSTRLSDSGEALASISANRH
                     TEPAKLSKLMRGELDWIVMKTLEKDRNRRYETANGFAADVQRYLDDEPVQACPPSAWY
                     RFRKFARRNRRAVITTTVLVVAMLIAVGAVGGSIGWASRDRAARQAAVEQEAGLATKE
                     AERWQEQGKWPEALSAVKRAEGLLAGSGSAELRDRVHQLRKDLEMVLRLEELRLPDPD
                     GKSFQLGDDAAYAHAFSDYGIDVASLPAVEVAARIRARPAVAVALAIALDDWANSRTP
                     NDEAGARKLTALAQAADPDPWRRQVRQAAQQNDTKALAGLAASPELVRQPPTSLLILT
                     SALRRVRLWNLYTQVLRQAQRQYPGDFWINFYLGVALNGPTTRGEGIGFYRAALAVRP
                     QNAMVLDHLGNALTKQNQLDDAIACHRKEIELSPKSHNAHNSLGVALMAQGKLDEAIV
                     SYHKAIEFLPEWAIPHYNLGCALFDQKKLDQAEACFRKAIALDPKYAPAHFNLGLIFH
                     RQKKLPEALAAYENAVQLDPKNAQAHGNLGAVLVD"
     gene            2312..>2552
                     /locus_tag="VFV87_06530"
     CDS             2312..>2552
                     /locus_tag="VFV87_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103447.1"
                     /translation="MHTGHWCALEKTTRLFVGSKKTFHTCMQLGVIPTRKGEEGLTLA
                     NIGEVQRFLENLAFVHNRVPGRKKLPPEDNASFRRE"
BASE COUNT          420 a          813 c          806 g          513 t
ORIGIN      
        1 ggtcgaccaa gacggcaccg aggttgccgt gggcctgcgc atttttcgga tcgagttgga
       61 cggcgttttc gtaggcggcg agggcttcgg gcagcttctt ttggcgatga aagataaggc
      121 cgaggttgaa gtgggcaggg gcgtatttcg ggtcaagcgc gatcgccttg cggaagcaag
      181 cttcggcctg atccagcttc ttctggtcga aaagtgcgca gccgaggttg tagtggggaa
      241 tggcccattc cgggaggaat tcaatggcct tgtggtagga gacgatggcc tcatccagct
      301 tcccctgagc catcaaggcg acgccaaggc tgttgtgggc gttgtggctt ttcggcgaca
      361 attcgatttc cttgcggtgg caggcgatgg catcgtccaa ctggttctgc tttgtcaggg
      421 cgttgccaag gtggtcgagc accatggcgt tctgcggacg gacggccaac gccgcccgat
      481 aaaagccaat tccctcgccg cgggtggtgg gcccgttgag agccacgccc agatagaagt
      541 tgatccagaa gtcgccggga tattgccgct gggcctgccg cagcacctgc gtgtacaagt
      601 tccataggcg aacacgacgc aaagcacttg tgaggatcaa gaggctggtt ggcggctggc
      661 gtacaagctc cggcgaggct gccagccccg ccagcgcctt ggtatcgttc tgctgggccg
      721 cttggcgcac ctgccgccgc cacggatcgg ggtcagccgc ctgggccagc gccgtcagtt
      781 tccgggcgcc tgcctcatca tttggtgtgc gggaattggc ccagtcatcc agggcgatgg
      841 ccagggcgac tgccactgcc ggccgggcgc ggatgcgagc tgccacttcc accgccggca
      901 agctcgcgac atcgattccg tagtcgctga aggcgtgcgc atatgccgca tcgtcaccta
      961 gttgaaagga ctttccgtcg ggatcaggaa gccggagttc ttccagccgc aggaccatct
     1021 ccaggtcttt gcggagttgg tgcactcggt cgcggagttc ggcgctgccg ctaccggcca
     1081 acagtccttc cgcccgcttg acggccgaaa gcgcctccgg ccacttgcct tgttcctgcc
     1141 accgctccgc ctctttcgtc gccaggccag cttcctgttc gaccgccgcc tgccgggccg
     1201 cccggtctcg cgacgcccag ccgatgctcc cccccactgc cccgaccgcg atcaacatcg
     1261 cgacgaccag gaccgtcgtg gtaatcacgg cccggcggtt ccgccgcgcg aacttgcgga
     1321 agcgatacca cgccgatgga gggcacgcct gcaccggctc gtcgtccaaa tagcgttgca
     1381 cgtcggccgc gaagccgttc gcagtctcgt accgccggtt gcggtctttc tccagagtct
     1441 tcatcacaat ccaatccaat tcgccgcgca tcagcttcga gagcttggcc ggttccgtgt
     1501 ggcgattggc cgagatcgac gccagcgctt cgcccgagtc gctcagccgc gtgctcggct
     1561 tgggcggctc ctcttccttg atcatgcgga gaatctcggc ataggccgct tccttcatcc
     1621 gcttgtgcgt cagcggcgtg ctgccggtca gcagctcgta caggagcacg cccagcgagt
     1681 agatgtcgct gcgggtatcg acgcccaggg cgctcatctc ggcctgctcc gggctcatgt
     1741 actcgagcgt gccgaccatg gtgccgtact gtgtgaacag ggtccgctcg gtcagcttct
     1801 gctcggtcgc cttggcgacg ccgaagtcga tgaccttggg caccggctgg ccgtcgtaga
     1861 gcgtgaccat gacgttggac ggcttgatgt cgcggtggat gatgcctttc tgatgagcat
     1921 gctgaatcgc ctggcagacc ggaacgaaga gctccagccg ctgccgcggc gtcaggtggt
     1981 tgtcatcgca gtacttcgtg atcggcacgc cgtggactag ctccatgacg aagtacgggc
     2041 ggccttgctc cgtggtaccg gcgtcgagca cgcgggcgat gttgacgtgg tccatgatcg
     2101 ccagggcctg gcgctcggct tcaaagcggg cgatcacctg ccgcgtgtcc atccccggct
     2161 tgatgatctt gagggccacc cgccgctcga ccggctcctt ctgctcggcc atatagacca
     2221 cgcccatccc cccttcgccg atctgctgga ggagcttgta cgggccgatc tgcgtgccgg
     2281 gattttcagt tacgggttga tcgaaggtta cttgcacacc ggccactggt gcgctctgga
     2341 gaaaactacc cgcctgttcg tgggatcgaa gaagaccttc cacacgtgca tgcaactcgg
     2401 ggtcatcccc acacgcaagg gcgaggaagg cctcacgctc gccaacatcg gagaagtcca
     2461 acgatttctc gaaaatctcg cgttcgtcca taaccgtgtt cccggccgca aaaagctgcc
     2521 tccagaggac aatgcaagtt tccgccgcga ac
//
LOCUS       DASUGB010000340         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1427317, whole genome shotgun sequence.
ACCESSION   DASUGB010000340 DASUGB010000000
VERSION     DASUGB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427317"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1049
                     /locus_tag="VFV87_06535"
     CDS             114..1049
                     /locus_tag="VFV87_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5103448.1"
                     /translation="MRDELMYRFLQSVVGLGCWSIALAASAGEPMVIDLWPGPAPGDV
                     GIEGEETTRIYQSPILGGPTKLITNVTRPALTIYQPPADKNTGTAMIICPGGGYHNLF
                     WELEGEEVAAWLNSQGMTGVILKYRCPRRKGDILGEPPLGPLLDAQRAVSLVRSRAKE
                     WGIHPQRIGMVGFSAGGHLSLATATRYEKREYEALDASDEASCRPDFAVLCYSGYLKS
                     KERDELAAGLQVPAQTPPIFLAHSTDDSISNAEHSVFMYLALKRAKIPVELHIYASGE
                     HDFGVRQNGKLPASWTGLCLNWLRSLDLLAPQARE"
     gene            complement(1056..1619)
                     /locus_tag="VFV87_06540"
     CDS             complement(1056..1619)
                     /locus_tag="VFV87_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103449.1"
                     /translation="MRFLVAIKPLLVLIGAIFGAAVGGRQVYIYWTERRPTPFMAESF
                     AAEYAGQQWVSVTGRMAVEARSEMPNHSRPGWVSVYVPLVPPDWQRQDPVHVVASSAA
                     MPAAQANAWAQTLGGQDQVTFTGVIRPLGGLNYPYVFPRLKFARPTITINENTQPAAP
                     WGMTAFLTICLVIGALAAWRVWWLLRT"
     gene            1796..2362
                     /locus_tag="VFV87_06545"
     CDS             1796..2362
                     /locus_tag="VFV87_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103450.1"
                     /translation="MQNKVFRLLAAWTLFFLAAMPAAAQVRFSIGAGGVSVGNAPRYG
                     YNPYRYGPGYPYGGRSYPGGTVVIGRGYSAPVTRYVERAPVAYAVPGVMIRNPEATGV
                     ALSYTIDSRHAYEIAAGEAQQLPEKGSYLVEFDRGGEFGQARYTITEGLYEFALTDHG
                     WELYRQPDSATSDQRVKANPLPELATPE"
     gene            complement(2366..3625)
                     /locus_tag="VFV87_06550"
     CDS             complement(2366..3625)
                     /locus_tag="VFV87_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103451.1"
                     /translation="MRRSQRVLLLAAFLLPACLGPLQAAEHLIRPGDSPQEILDRADP
                     GDRLVFLPGLHEHGLSQHRAILYVDKSVEIELREGATLKLAAGVTKLEAEGEITTDQD
                     AAKMLDDLEIGGQFDLSRPAADGPELYGNTIYTIVIDGEGVGGQSDTFAWGDGKLFDT
                     PHKQVPITGEWQDLSHGVKIRFASRTGHSLRSLWFVSYDGPEAYGIRIGHGRQQEYIE
                     NVRIVGKGTIDMNASHNALPSGLVKNINACVLIHGRVRGVLVDGITMTDTMRSVMAYG
                     EHTGDFLPGGKVGPGESFDAENITVQYTRTINPAGSGYLFGHPSFRGQIRNIRCNHNY
                     METALTAIEPNFNLDGYEVIGNVIKSGGQAIHCWRHSKNGVVADNLRIHDNTGKPVVV
                     VNSPRGWAPPEPPVLRNNRNHLSDRMP"
     gene            complement(3649..>4024)
                     /locus_tag="VFV87_06555"
     CDS             complement(3649..>4024)
                     /locus_tag="VFV87_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103452.1"
                     /translation="YWPLRATVTYLGMMVPVALVAIPMAIVATIDSPRRIPGVIAVVL
                     WALIVRLATDLIYYGLIDPPVSMRPQWMKFICSEAIEVGSLLAVTGATFAVLYWFGYR
                     LAGPGNRNAKTGAAVRAVHSGE"
BASE COUNT          723 a         1205 c         1353 g          743 t
ORIGIN      
        1 gatggttccc tggatttgcg ggtgaactcg tgcgcgggcg atcggctaaa atatcgagca
       61 acgcaggcgc tccatcgcga cttcaaaagg gcttgaatcg ctggactacg tatatgcggg
      121 acgaacttat gtatcgattt ctgcagtcgg tcgttggcct tggttgctgg tcgatcgcgc
      181 tggctgcttc ggccggcgag ccgatggtca tcgacctttg gccagggccg gcgccgggcg
      241 atgtggggat tgaaggcgag gagacgactc ggatttacca atcgccgatc ctcggcgggc
      301 cgacgaagct gattaccaac gtcaccaggc cggcacttac gatctatcag ccgccggccg
      361 acaagaacac cggcacggcc atgatcatct gccccggggg cgggtatcac aacctgttct
      421 gggaactgga gggggaagag gttgcggcgt ggctgaactc gcaggggatg acgggcgtca
      481 tcctgaaata tcgctgcccg cggcggaaag gcgacatcct gggcgagccg ccgctggggc
      541 ctttgctcga cgcccagcgg gcggtcagcc tcgtccgcag ccgggcgaag gagtggggca
      601 ttcatcccca gcggatcggc atggtcggat tttcggccgg cgggcatctt tcgctggcga
      661 cggcgacacg ctatgagaag cgtgagtacg aagcgctcga cgccagcgac gaagcgagct
      721 gccggcccga ttttgccgtg ctctgttatt cggggtacct caagtcgaag gagcgggacg
      781 agctggcggc agggctgcaa gttcccgccc agacgccgcc gattttcctg gcccattcca
      841 cggacgattc gatcagcaac gccgagcaca gcgttttcat gtatctggcg ctcaagcggg
      901 cgaagatccc agtggagctg cacatctatg ccagcggcga gcacgacttc ggtgtccggc
      961 aaaacggcaa gctgccggcg agctggaccg gcctgtgcct gaactggctc cgcagcctgg
     1021 acctgcttgc gccgcaggcg cgtgagtgaa cgcactcacg tgcggagcag ccaccacacg
     1081 cgccaggcgg cgagcgcgcc gatgacgagg caaatcgtta gaaaggccgt catgccccag
     1141 ggggcggcgg gttgggtgtt ttcgttgatc gtgatcgtcg gccgggcgaa tttgagtcgc
     1201 ggaaagacgt acgggtaatt caggccgccc agcgggcgga tgacgccggt gaaggtgacc
     1261 tggtcctggc cgccgagcgt ttgggcccag gcgttcgcct gggccgcggg catcgcggcg
     1321 gaactggcca cgacgtgcac cgggtcttgc cgctgccagt ccggcggcac gagcgggaca
     1381 tacacgctca cccagccggg ccggctgtga ttgggcattt cgctccgcgc ttcgacggcc
     1441 atccgcccgg tgacgctgac ccactgttgg ccggcgtatt ccgcggcgaa gctttcggcc
     1501 atgaaggggg tcggccggcg ctccgtccaa tagatataga cttggcgtcc gcccactgcg
     1561 gcgccgaaga tcgcaccgat aagcaccagc agcggcttga tggcgacgag gaagcgcatg
     1621 acggttggtg cgggatattc cgagcaacga ggcgattatg agaccgagga gttgccgccg
     1681 ccagactccc ccacccctcg aatcttggct gttcgtcaca gatggatcac cggccgctgg
     1741 aggaatcggc gcgatgcagc tacgtcgatc atttcgatag gcagaggagt gtgtcatgca
     1801 aaacaaggtg tttagattgt tggcggcttg gactttgttc ttcctggcgg ccatgcctgc
     1861 ggcggcgcag gtgcgatttt ccatcggagc gggtggcgtc tcggtaggaa atgcgccgcg
     1921 gtatgggtac aacccctacc ggtatggccc cggctatccg tacggcggac ggtcgtatcc
     1981 tggcggcaca gtagtgatcg gtcgcggcta ttccgcgccg gtcacgcgct acgtcgagcg
     2041 ggcgcccgtg gcgtatgccg taccgggggt gatgatccgc aacccggaag cgaccggtgt
     2101 tgcgctgagc tatacgattg attcgcgtca cgcctacgaa attgcagcgg gcgaggcgca
     2161 gcagctcccc gaaaagggga gctacctcgt cgagttcgac cgcggcggcg aattcggcca
     2221 ggcccgctac acgatcaccg agggactgta tgaattcgcc ctcaccgatc acggctggga
     2281 gttgtatcgc cagccggact ccgcgacgtc agaccagcgc gtgaaggcca acccgctgcc
     2341 cgaactcgcc acgcccgaat aaaggtcaag gcattcggtc gctgaggtgg ttgcggttgt
     2401 tccgcaggac gggcggctcg ggcggggccc agccgcgggg ggaattaacg acgacgaccg
     2461 gcttgcccgt gttgtcgtgg atgcggaggt tgtcggcgac gacgccattt ttggagtgcc
     2521 gccagcaatg aatcgcctgg ccgccgctct tgatgacgtt gccgataacc tcgtagccat
     2581 cgagattgaa attcggctcg atcgcggtca gggcggtctc catgtagttg tgattgcagc
     2641 ggatgttgcg aatctggccg cggaacgacg gatgcccgaa cagatagccg ctgccggcgg
     2701 gattgatggt gcgcgtgtat tgaacggtga tgttctcggc gtcgaacgat tcgccggggc
     2761 cgactttgcc ccccggcagg aagtcgccgg tgtgctcgcc gtaggccatc accgaccgca
     2821 tcgtatcggt catcgtgatg ccatccacga gcacgccgcg cacgcggccg tggatgagga
     2881 cgcaggcgtt gatgttcttc accaggccgc tggggagggc gttgtgcgag gcattcatgt
     2941 cgatcgtccc tttgccgacg atccgcacgt tctcgatgta ctcctgctgg cggccgtgcc
     3001 cgatgcggat gccgtaggct tccgggccgt cgtacgagac gaaccacagg ctgcggaggc
     3061 tgtgcccggt gcggctggcg aagcggattt tcacgccgtg gctgagatcc tgccactcgc
     3121 cggtgatcgg cacttgcttg tgcggcgtgt cgaacagctt gccgtcgccc caggcgaacg
     3181 tgtcgctctg accgccgacg ccttcgccgt cgatgacgat cgtgtaaatc gtgttgccgt
     3241 acagctccgg gccgtcggct gcggggcgcg agagatcgaa ctggccgccg atctccagat
     3301 cgtcgagcat cttggcggcg tcctggtcgg tggtgatctc cccctcggct tcgagcttcg
     3361 tgacgcccgc cgcgagcttc agcgtcgccc cttctcgcag ctcgatctcg accgatttat
     3421 cgacatagag gatcgcccgg tgctgcgaca gcccgtgctc gtgcaatccg ggcagaaaga
     3481 ccagccgatc gccgggatcg gcgcggtcga gaatctcctg cggtgaatcg ccggggcgga
     3541 tgagatgctc ggcggcttga agcgggccga ggcacgctgg gagaaggaac gcggctaaga
     3601 gcagtacacg ttgagatcgt ctcatcgagt tcaaccttga tgaagctgct attcgccaga
     3661 atgaacggct cgaactgcag cgcccgtttt cgcgtttctg tttcctggcc ccgcgagccg
     3721 ataaccgaac cagtaaagca ctgcgaaagt tgcgcccgtg accgcaagca gactccccac
     3781 ctctatggct tcggagcaga taaacttcat ccactgcgga cgcatgctaa cgggcgggtc
     3841 gatcagaccg tagtaaatca ggtcggtcgc cagacgaacg ataagggccc acaagacaac
     3901 ggcaatgaca cctggtatcc ggcgtggcga atcaatcgtc gcgacgattg ccatcggaat
     3961 ggcgacgagc gcaactggca ccatcattcc gagataggtc acagttgcac gaagcggcca
     4021 atac
//
LOCUS       DASUGB010000341         7496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_455878, whole genome shotgun sequence.
ACCESSION   DASUGB010000341 DASUGB010000000
VERSION     DASUGB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455878"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VFV87_06560"
     CDS             complement(<1..1518)
                     /locus_tag="VFV87_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103453.1"
                     /translation="MPRLLALEWDQKEARVAVAHARGAGIVVEHAFVVPLVEREHSAV
                     LEADVGAAIGKALAERGLSRTEALVAVGRASIELRFLTTPPVPEDELPDIVRFQALRQ
                     FTTLGDDWPLDFVPLAPNADGGTNVLAAAISPDLVNQIRQTATAANVSVTGLVLRPFA
                     AASLLRKEHADGKCRMIVDLLRDEADMTVLLGPQVIFPRTVRLPTVAEPEALARALLA
                     EGRRTMIAAQNQLGGRRVEEVVIFGDGQHHSSLKQLLEKELSLTVKLIDPFEWVEWDG
                     GARAQKPEYPGTFAPLLGVLCDAARETRPEIDFLHPRKKPEPPNRRRTMLIAGGAIAA
                     LVLIGFAWMQLSLWSLDGRIAQLKKDVASQKKLADKSVKPVEQADRLEQFSAGDVTWL
                     DELALMSKRFPKAEAARVREMHWRMDSKAGGGKTTLKVLADKPATITATERALRDKRH
                     SVLGSGATEDDQAPQPLQWASTADVSVEPGVPDPAEKIALEPATPGSSAPAAGATT"
     gene            complement(1608..3140)
                     /locus_tag="VFV87_06565"
     CDS             complement(1608..3140)
                     /locus_tag="VFV87_06565"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HEX5103454.1"
                     /translation="MTESGKRRALVLILVLVVIVMLSLGAYSFTDLMIAHHEASQLTG
                     RQVQTRALVDSGVEVVRVFLMQTEAEQLEAGGVFDNPDRFRGVNVVPDDDPNLRGSFT
                     VLSPNLDDQGYLAGTRYGLEDESTRLNLNTLLAAEKTTPDAGRTFLMALPGMTEDVAD
                     AILDWLDADDEPRELGAEIDYYSGLNPPYAPQNGPLDTVEELLLVRGVTPQLLFGADV
                     NRNGQLDPHEITEDGSGGSTDPEGFRGWSAYLTLHSLEWNVNPDGQPRVYLNQNDMQK
                     LYDELVAVLPEEWATYIIAYRQSGPYTSTIRTATSAAGKQLDLTKPAKTPISQVIELV
                     GSNVRHSFVGDQTPSFITSPFPDDIGAMNVYLPMLMDYVTVNPAPTIPGRINMNQASA
                     TVLAGIPGMTPEIVEQIISLRDVAPSSDRPARRHETWLLQEVVVDLPTMKLMMPFVTC
                     GGDVYRAQVVGYYQGGQAASRSEVIFDATSPLPRLLFWRDISHLGRGYAVETLGVDFS
                     EE"
     gene            complement(3176..4915)
                     /locus_tag="VFV87_06570"
     CDS             complement(3176..4915)
                     /locus_tag="VFV87_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103455.1"
                     /translation="MQVAAGRGQFQVCLAARAASADWVEPADKVAALGRAAGKTTTAM
                     AVRGGAGKATAMGVAVAADEEAAAEVLEVLVAVGRGAPVAEDLAAGAAQAEEVQAEAD
                     REEAGDRAEEVGQEVADSGVVVAALGGQVAAVQVAAVQVAVAVASAAQVAKEVPVAKG
                     RRKWIFCRRGLTLLELILALALSVLVLMAIGMAINLHYRMFDVRRTNIEEAHVARATL
                     RIIADDLRGAVWYVPPDLSGLDTVAGNTASAAANLATSQIQSAAAGAGVNLGGTATGA
                     GGSSGSGTTSGSQTGASPTGFQQFTQATQGTQSSQGTQSGQNGSSSGGNAQTGGTTGV
                     AGFGNGFTGTTPQTGSTATAATANGTTEETAELGTPITVVGLYGSTSTLQFDVSRLPR
                     VDEYEAIMSPTSEMGVVDIPSDIKTVTYFVASEATTDASQAGGLFAGSPEPSATGRGR
                     GLMRSELSHAVSAWAESNGNLNSTYANAKLLADEVVGLQFRYFDGTGWYDDWNSDDLG
                     CLPSAVEITVTIQPTYGMSEEDIAELAVDELPPERMYSLIVNLPAALPPEPAAPVDTA
                     AGDSAVMEEEVLP"
     gene            complement(5420..6016)
                     /locus_tag="VFV87_06575"
     CDS             complement(5420..6016)
                     /locus_tag="VFV87_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5103456.1"
                     /translation="MRRGFTLLEMLLVLAILVAVAALAAPALKGVIEGAKLRSAADTV
                     RTEWMRAHVKAMKTGRIQVFRYEIGGTKYTIQPWIAGDDALESSAPAVHGFEVADEAQ
                     DTANTGMEHELPEGTTFVGGDALVESRSLAIEQATADSTSSAAEWSRPILFYPDGSTS
                     DAYVIVGNERKVGLRVGLRGMTGAASVSDVMELEEGGP"
     gene            complement(6099..6515)
                     /gene="gspG"
                     /locus_tag="VFV87_06580"
     CDS             complement(6099..6515)
                     /gene="gspG"
                     /locus_tag="VFV87_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX5103457.1"
                     /translation="MKRREFRRGFTLMEVLLVLAILVILGSLVGVGIMRMQKNANISA
                     TRSQISMLEDVVNAYAIDVGTCPTTQQGLQALVAPPSDLKNPAKWQGPYLEKQLGQDP
                     WGNDYQYEQTGPDQFRIWSIGPDLASGSEDDIANVQ"
     gene            complement(6625..>7496)
                     /locus_tag="VFV87_06585"
     CDS             complement(6625..>7496)
                     /locus_tag="VFV87_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5103458.1"
                     /translation="ADAMARFPRAFSEMAVNMVRAGGEGGFLEDALERVASFTEQQED
                     LKGRTISAMVYPLLLGTAGTVVVTGLIVFFVPKFAVMFERLRQRGELPWATDMLLWCS
                     DTIREWGWLILLVGVIAVVWARFRFSTPEGRKLADLIKIKVPVAGHIFLSFAVARFCR
                     VLGTLLKNGVPILKALEISRDASGNRVLSEAIAKASENISGGHSLAGPLAGSGHFPKM
                     VVEMIAVAEESNTLDKVLVDLADDLEKRTSRQLDLAVRLLEPIMLIILAVIVLIVVIA
                     LLVPVMKMSSAMG"
BASE COUNT         1301 a         2525 c         2263 g         1407 t
ORIGIN      
        1 agtcgtagcg cctgctgctg gtgcagagga acctggcgtg gccggttcga gcgcgatttt
       61 ttcagcaggg tcgggaacac cgggctcaac ggacacgtca gccgtcgagg cccactgaag
      121 cggctgcggc gcctggtcgt cctccgtcgc cccagaaccg aggaccgagt gccgtttgtc
      181 gcggagcgcg cgttccgtcg cggtaatcgt cgcgggctta tcggcgagca ccttgagcgt
      241 cgttttgccg ccgccggcct tggaatccat tcgccagtgc atttcgcgga ccctcgccgc
      301 ttccgctttg gggaaccgct tgctcatgag ggccagctca tcgagccagg tcacgtcgcc
      361 ggcggaaaac tgctccagcc ggtccgcctg ctcgacgggc ttaacgctct tatctgccag
      421 ctttttctgg ctggcgacgt ccttcttgag ctgggcgatg cggccatcca gcgaccacag
      481 gctgagctgc atccatgcaa agccgatgag gaccagcgcc gcgatcgcgc cgccggcaat
      541 cagcatcgta cggcggcgat tggggggctc gggttttttt cgcgggtgca gaaagtcgat
      601 ttccggccgc gtttcccggg ccgcgtcgca caacacgccc agcagcggcg cgaacgtccc
      661 gggatattcg ggtttctgcg cccgcgcgcc gccgtcccat tcgacccatt caaagggatc
      721 gatcagcttg accgtgagcg agagctcctt ctccagaagc tgcttaagcg agctgtggtg
      781 ctggccgtct ccgaaaatca cgacctcttc cacgcgccgg ccgccgagct ggttttgcgc
      841 ggcgatcatc gtccggcggc cttcggccaa gagcgcccgg gccagggctt ccggctccgc
      901 aacggttgga aggcggaccg tgcgagggaa gatcacctga gggcccagga gaaccgtcat
      961 gtccgcttcg tcgcgcaata gatcgacgat catccggcac ttgccgtccg cgtgctcttt
     1021 ccgcaacagc gaggccgccg cgaatggccg cagcactagc cccgtgacgc tgacgttggc
     1081 cgcggtcgcg gtctgacgaa tctggtttac cagatcggga gagatggctg cggccagcac
     1141 gttggtgccg ccgtcggcgt tgggcgccag cggaacgaaa tcgagcggcc agtcgtcgcc
     1201 taaggttgtg aactgccgga gggcctgaaa gcgaacgatg tcgggcagct cgtcctcagg
     1261 aacgggcggc gttgtgagaa accgcagctc gatgctcgcc cggccgacgg ctaccagggc
     1321 ttccgttcgc gacaggccgc gctccgccag cgctttgccg atcgccgcgc ccacatccgc
     1381 ttccagcact gcggaatgct cgcgctcaac gagcgggacg acgaacgcat gctcgacgac
     1441 gattcctgca ccacgtgcgt gggccacggc cacgcgggct tctttttgat cccattcgag
     1501 ggctaacaac cgaggcattg ctcattggtc atttgtcatt tgtcatttgt cacttgtcat
     1561 ttgttgagac tgcaaatgac caatgaccaa tgaccaatga caaacaacta ttcctccgaa
     1621 aaatcgactc ccagcgtctc cacggcgtag ccgcggccga gatggctgat atctctccag
     1681 aataatagcc gcggcagggg gctggtagcg tcgaaaatga cctccgaccg cgaagccgcc
     1741 tgccccccct ggtaatatcc aaccacctga gcccggtaca cgtcgccgcc gcaggtgaca
     1801 aacggcatca tcagcttcat cgtaggcaga tcgacaacca cttcctgcag cagccaggtt
     1861 tcatgccgcc gggccggccg gtcgctgctg ggggccacgt cgcggagcga aatgatttgc
     1921 tcgacgattt ccggcgtcat gcctggaatt ccggccagaa cggtggccga agcctgattc
     1981 atgttgattc gccccggaat tgtgggggcc ggattaacgg tcacatagtc catcagcatc
     2041 ggcagataga cattcatcgc accgatgtcg tcggggaagg gggacgtgat gaacgacggc
     2101 gtctgatccc cgacgaacga atgccgcacg ttggagccaa ccagctcgat cacctgcgag
     2161 atgggcgtct tggcaggctt tgtcagatca agctgtttgc ccgctgccga ggtagcggtg
     2221 cggatggtgc tcgtgtaggg accgctttgg cgataggcga tgatgtacgt ggcccattcc
     2281 tcggggagca ccgcaaccag ctcgtcgtac agcttctgca tgtcgttctg gttcagatag
     2341 acccgtggct ggccgtcggg attgacgttc cattccaggc tgtgcagcgt gagataagcc
     2401 gaccagccgc ggaagccttc cgggtcggtc gagccgccgc tgccgtcctc ggtgatctcg
     2461 tgcggatcga gttgaccgtt gcgattgaca tcggctccaa acaggagctg cggtgtgacg
     2521 ccgcgcacga ggagcaattc ctcgaccgtg tcgagcggcc cgttctgcgg cgcgtaaggc
     2581 ggattgaggc ccgagtagta gtcgatctcc gcccccagct cgcgcggctc gtcgtcggca
     2641 tcgagccagt cgaggatcgc gtcggcaacg tcttcggtca tgccgggaag cgccatcaag
     2701 aacgtgcggc ccgcatcggg agtggtcttt tccgcggcca aaagcgtgtt gagattcagc
     2761 cgcgtcgatt cgtcttccag gccgtagcgc gtgccggcca ggtagccctg atcgtctaga
     2821 ttcggcgaaa gcactgtaaa gctgccgcga aggttgggat cgtcgtcggg gacgacgttc
     2881 acgccgcgga accgatcggg attgtcgaat acgccccctg cttcgagctg ctcggcttcg
     2941 gtttgcatga ggaaaacgcg gacgacctcc acgcctgaat cgactagcgc ccgggtctgc
     3001 acctgccggc cagtgagctg cgaagcctcg tggtgcgcaa tcatcaggtc cgtgaatgag
     3061 tacgcgccca gcgacagcat cacgatcacg accagcacca gaatcaaaac cagcgcccgc
     3121 cgcttcccag actctgtcat ctgccttctg tcttctgtcc tattgcctcc ggctctcacg
     3181 gcagcacctc ctcttccatg acggccgaat cgccggctgc cgtatcgacg ggcgcggccg
     3241 gttcaggagg cagggccgcc ggtaagttga cgatcaggct gtacatcctc tccggcggca
     3301 gctcatcgac ggctagctcg gcaatgtctt cttcgctcat gccatacgtc ggctggatcg
     3361 tgaccgtaat ttccaccgcg ctcggcaggc agcccagatc gtcggaattc cagtcgtcgt
     3421 accagccggt gccgtcgaag tagcggaatt gcagtcctac cacctcgtcg gccagcagct
     3481 tcgcgttggc ataggtggag ttcaagttgc cgttggactc ggcccaggcg ctgacagcgt
     3541 ggctcaattc gctgcgcatc agtccgcgcc cgcggcccgt ggcgctcggc tccggcgagc
     3601 ccgcaaacag gccgcccgcc tgcgatgcat cggtcgtcgc ttccgacgcg acgaagtacg
     3661 tgaccgtctt gatgtcgctg ggaatatcga cgacgcccat ctcggacgtg ggcgacatga
     3721 tcgcttcgta ttcatcgacc cgcggcaggc ggctgacgtc gaactgcagc gtgctcgtgc
     3781 tgccgtacag gccgacgacg gtgatcggcg tgcccagctc ggccgtttcc tcagtcgtgc
     3841 cattcgccgt ggcagcggtc gcggtcgatc cagtctgcgg cgtcgttccc gtgaatccat
     3901 tcccaaagcc ggcgacgccg gtggtgccgc ccgtttgagc gtttccgccg ctggaagagc
     3961 cgttctgccc gctctgggtg ccttgtgacg actgcgtgcc ttgtgtcgcc tgtgtgaact
     4021 gctggaagcc cgtcggggat gcgcccgtct gactgccgct ggttgtgcca cttccactgc
     4081 tgccgccggc gcccgtggcc gtgccgccaa ggttcacgcc cgccccggct gcggcgctct
     4141 ggatttgcga cgtcgcgagg ttggctgccg cgctggccgt gttgccggcg acagtatcca
     4201 gacccgaaag atcgggcggg acgtaccaga ccgctccgcg gagatcgtcg gcaatgatcc
     4261 gcagcgtggc gcgggcgacg tgggcctctt caatgttcgt ccggcgcacg tcgaacatgc
     4321 ggtagtgcag gttgatcgcc atgccgatcg ccatcagcac gagcaccgag agggctagcg
     4381 cgaggattaa ctccaagagc gtcagaccgc gtcggcaaaa tatccatttg cgacggcctt
     4441 tagccacggg gacctccttg gccacctggg ccgccgaagc caccgccacc gccacctgga
     4501 ccgccgccac ctggaccgcc gccacctggc cccccaaagc cgccaccacg acccccgaat
     4561 ccgccacctc ctggcccacc tcctccgccc ggtccccggc ctcctcccgg tccgcctccg
     4621 cctggacctc ctccgcctgg gccgcgcccg ccgccaaatc ctccgccacc ggggcccccc
     4681 tgcccaccgc caccaggacc tccaaaacct ccgccgctgc ctcctcgtcc gccgcgaccg
     4741 ccacgcccat tgccgtcgcc ttgcccgccc cgccccggac cgccattgcc gttgttgttt
     4801 tgcccgccgc ccgccccaaa gccgccacct tgtccgccgg ctccacccag tccgccgagg
     4861 ccgcccgtgc cgccaggcag acctggaatt gcccccgtcc cgccgccacc tgcatttaga
     4921 ttcgttgccg ccccagtcgt cggagctgcg cctccctgcg agctgctctg cgccgcggcc
     4981 agggcttctt gagcctgggc cttcatctca gccgcagccg cggctgcggc ctcttcgacc
     5041 gccggatcga gaatccaccg cgaaagcgag aaggagacgg gccgggcgac cgattccggg
     5101 tcctgtgtca cggtgacgcg caccgcgagc atgcccgatt cgtccgtctc ttgcggctcg
     5161 acgaagtaca cccactcgcc caagtcgtcg agtggctccg cgctgactgc ggccgtcggg
     5221 tcctccacgc cggcagccag ggcattgagc tttgtttcgc aatagatctg tgccgtcgta
     5281 agatcgcggg ccgctgccgc gttgcgagcg ccgatgcgaa cgagctcgcc gatgaccgcc
     5341 aacgcgccgc caagaatggc gatcgccagg atcacctcca ggagcgacaa gccgcgacga
     5401 ctctttttgc gaaacgctct catggaccgc cctcctccag ttccatgacg tcgctcaccg
     5461 aagcggctcc tgtcattccc cgcagcccaa cacgcagccc gaccttccgc tcatttccga
     5521 cgataacgta cgcatccgat gtcgagccgt caggatagaa gagaatgggc cgcgaccact
     5581 cggctgcgga cgacgtggag tccgcggtgg cctgctcaat agccagcgag cggctttcga
     5641 cgagggcgtc gccgccgaca aacgtcgttc cctcgggcaa ctcgtgttcc atgccggtat
     5701 tggccgtatc ctgcgcttcg tcggcgacct caaagccatg gacggccggc gcgctggatt
     5761 ccagggcatc gtcgccggcg atccagggct gaatggtgta tttcgtgccg ccgatctcgt
     5821 aacggaagac ctggatgcgg ccggtcttca tcgccttgac gtgggcccgc atccactcgg
     5881 tgcggacggt gtcggcggcg gaacgcagct tggccccttc gatcacccct ttgagcgccg
     5941 gcgccgcgag cgcggccacg gcgacgagaa tcgccaagac cagcagcatc tcgagaaggg
     6001 tgaagccgcg acgcataggt ttgtcagtcg tcagttgtcc gtagtcagtt ggatcgttcc
     6061 ccaccgacta ctgaccactg actactgacc aatggcgatt actgcacgtt ggcgatgtcg
     6121 tcctccgagc cgctagctag gtcggggccg atcgaccaga ttcggaactg gtccggacca
     6181 gtctgctcgt actgatagtc gttgccccac ggatcctggc caagctgctt ttcgaggtag
     6241 gggccttgcc acttggctgg gttcttcagg tcggacggag gggcgacgag ggcctgcaat
     6301 ccctgctgcg tggttgggca cgtccccacg tcgatcgcgt acgcattgac cacgtcttcg
     6361 agcatcgaga tttggctccg cgtcgcgcta atattcgcat tcttctgcat ccgcatgatt
     6421 ccaacaccca ccagcgaacc gaggatcacc aggatcgcca gcacgaggag gacttccatc
     6481 agcgtaaagc cgcggcggaa ttctcgacgt ttcatatccg attctccttc aactttcttg
     6541 ttcgttggca gttgtcagtg gtcagttgca gtcgtgtgga tccttccaac tttcaacgga
     6601 caacggacca ccgacaactg acaattaccc catagcgctg ctcatcttca tcaccggcac
     6661 cagcaaggca atcaccacga tcagcacaat caccgccagg atgatcagca tgatcggttc
     6721 caggaggcga acagccagat caagctgccg gctggtgcgt ttttccaggt cgtcggccag
     6781 atccaccagc actttatcca gggtgttcga ctcctcggcg accgcgatca tctccaccac
     6841 cattttgggg aaatgaccgc tgccagccaa tggcccggcc agcgaatgcc cgccggagat
     6901 gttctccgac gctttggcaa tcgcctccga gaggacacgg ttgccagagg catcgcggct
     6961 gatctcgagg gcctttaata ttggaacccc gtttttgagc aaagttccga ggacacggca
     7021 aaaacgggcc accgcaaagc tgagaaaaat gtgccccgcg acggggactt tgatcttgat
     7081 caggtcggcc agcttgcgtc cttcgggcgt gctgaagcga aaacgggccc acacgaccgc
     7141 aatcacgcca accagcaaga tgagccagcc ccactcgcgg attgtgtcgc tgcaccacag
     7201 gagcatatcg gtggcccagg gcaattcccc ccgctggcgc aggcgctcga acatcactgc
     7261 gaacttggga acgaagaaga caatcagccc cgtcacgacg acagtgcccg ctgttcccaa
     7321 gagcagcgga tagaccattg cgctgattgt gcgtcctttc aggtcttcct gctgctcggt
     7381 gaacgaggca acccgctcca gggcgtcttc caaaaaaccg ccttcgccgc cggcgcggac
     7441 catattgacg gccatttcgc tgaaggcccg cgggaagcga gccatcgcgt cggcca
//
LOCUS       DASUGB010000342         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_593537, whole genome shotgun sequence.
ACCESSION   DASUGB010000342 DASUGB010000000
VERSION     DASUGB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593537"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFV87_06590"
     CDS             complement(<1..378)
                     /locus_tag="VFV87_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5103459.1"
                     /translation="MKIADRHLSASPAAATASPGDCHVTDPTMGAGAYFRLKPWLDRA
                     LAALLLLPGLPLMGILILLVRLTSRGPGVYCQVRVGKSGKNFTMYKLRSMRLDAEAKS
                     GPAWSASGADPRVTRLGYWLRRLH"
     gene            complement(429..1112)
                     /locus_tag="VFV87_06595"
     CDS             complement(429..1112)
                     /locus_tag="VFV87_06595"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HEX5103460.1"
                     /translation="MKNLTFVVCSCILLVSTVAAGWVQRSMSHAWGNPRDRDAAAARL
                     STSLPEQIGDWRFDHAVPFPEDVLDVLGKPTHISHVYENMRTGDFVTVAVIVGHPGPV
                     AVHTPEICYSSRDYKFPSARTRQEIKAADGRAHALWTLGLDPKEAGDLPLKVMYAWST
                     GTTWEAAERPRYSYGGLSHLYKLQLAVGVTPQSSEAGFDPAGDFLTNFLAVLQGKLVE
                     SDHRQKAAK"
     gene            complement(1159..>2152)
                     /locus_tag="VFV87_06600"
     CDS             complement(1159..>2152)
                     /locus_tag="VFV87_06600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103461.1"
                     /translation="ALVVQMQLQARQHERCDKWLDQLAQAEPASLRPAALRAQVAVAR
                     DPSADVESLVEAKALEVAAAASNPTDQKKLLAGIGDLYASLKQYAPAERWYRKLVALA
                     PDQYPLLVGTLAKQGRLADAIAICEQAAKTETTSRPILVMVAALTDGTPGEQDYKQAE
                     PLLAAALARFDKEAGLLYSATLVRISQGRYDAAAELYRQILEINPKFVPALNNLAMLL
                     AERPAERGEAITLIDRAIEIAGKDAGLLDTKGAILTYSSRSAEAVPLLVAATRGAAAD
                     PRHHFHLAIAYRDQGKLEEAKVQLKTALERQLTQQVLMPTDQRLLAELRSALAL"
BASE COUNT          366 a          670 c          734 g          382 t
ORIGIN      
        1 gtgcaagcgg cggagccaat agccgagtcg cgtgacccgc ggatcggcgc ctgaagccga
       61 ccaggcaggg cccgatttgg cctcggcatc gagccgcatg ctgcgcagct tgtacatcgt
      121 gaagttcttt ccgctcttgc ccacgcgaac ctggcagtaa acgcccggcc cgcgcgaggt
      181 aaggcgtacc agcagaatca ggattcccat gagcggcagc cccggcagca gcaacagggc
      241 cgccagggcc cgatcgagcc agggcttgag ccggaaatac gcgcctgcgc ccatcgttgg
      301 atcagtcacg tggcaatcgc ctggcgatgc cgttgctgcc gcggggcttg cgctcaagtg
      361 cctatcggcg atcttcatgg gagcagatcc tgagtgacga ctgcgagatg acgacggatg
      421 aatgacagtc atttggcggc cttttgccgg tgatcgcttt cgaccaattt gccctgcaag
      481 acggcgagaa agtttgtgag gaaatcccca gccggatcga agccggcctc gctggactgg
      541 ggcgtgacgc ccaccgccag ctgcagcttg taaagatgcg acaggccgcc gtaggaataa
      601 cgaggccgct cggctgcttc ccaggtggtg ccggtgctcc aggcgtacat gacttttagc
      661 ggcaagtcac cggcctcttt ggggtccagg ccgagcgtcc acaaggcatg cgctcggccg
      721 tccgccgcct tgatctcttg gcgggtgcgg gcggaaggaa acttgtaatc gcggctggag
      781 tagcaaatct ccggcgtatg cacggcgacc ggccccggat gacccacgat cacggcgacg
      841 gtgacaaagt cgcccgtccg catgttttca tagacgtggc tgatgtgcgt cggcttgccc
      901 agcacgtcga ggacatcctc tggaaacgga accgcgtgat caaaccgcca atcgccgatt
      961 tgctcgggca acgaggtgga aagtcgggcc gctgccgcgt cgcggtcgcg gggattgccc
     1021 caggcgtgcg acatgctccg ctggacccag ccggcagcga cggtgctgac gagcagaatg
     1081 caggagcaga cgacgaacgt aaggtttttc atcgcaggaa gtgaggcgga ttgaatcgcg
     1141 gtgcagatgc ggcgactgct agagtgccaa ggcgctgcgc agctcggcca gcagacgctg
     1201 gtcggtgggc atcaggactt gctgggtcag ctggcgttcg agggccgttt tgagctggac
     1261 cttggcctcc tccagctttc cctggtcgcg gtaggcgatc gccaggtgga aatggtgccg
     1321 cggatcggca gccgcgccgc gggtggccgc gaccaacagc ggcacggcct cggccgaacg
     1381 gctgctgtag gtcaagatgg cgcccttggt gtcgagcagc ccggcgtcct tgccggcgat
     1441 ttcaatggcc cggtcgatga gcgtgatcgc ctcgccccgc tcggccggcc gctcggccag
     1501 cagcatcgcc aggttgttaa gggccgggac aaacttggga ttgatctcca ggatttggcg
     1561 atacagttcg gcggcggcgt cgtagcggcc ttgcgagatg cgtacgagcg tcgcggaata
     1621 caggaggccg gcttctttgt cgaagcgcgc cagagcggcg gcgagcagcg gctcggcttg
     1681 cttgtagtcc tgctcgccgg gcgtcccatc agtcagtgca gccaccatga ccaggatcgg
     1741 ccgcgacgtg gtttccgtct tggccgcctg ctcgcaaatc gcgatggcgt cggccagccg
     1801 gccttgcttg gccagcgtgc cgacgagcag cgggtactga tcgggggcaa gcgccaccaa
     1861 tttacgatac cagcgctcgg cgggtgcata ctgcttcagg ctggcgtaca ggtccccgat
     1921 gccggccagc agcttctttt gatccgtcgg attgctcgcg gcggcggcca cttccagggc
     1981 tttggcttcc acgagcgatt cgacatcagc gctcggatcg cgggcgacgg ccacttgcgc
     2041 tcgcagcgcg gccggccgca ggctcgcagg ctcggcctgg gcaagctggt caagccactt
     2101 gtcgcaccgc tcgtgctgcc tggcctgcag ctgcatctgg acaaccagcg cc
//
LOCUS       DASUGB010000343         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1012271, whole genome shotgun sequence.
ACCESSION   DASUGB010000343 DASUGB010000000
VERSION     DASUGB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012271"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFV87_06605"
     CDS             <1..704
                     /locus_tag="VFV87_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX5103462.1"
                     /translation="KRLAGEVNARWISDHLCWTGVAGRTTHDLLPLPLNEEALSHVVG
                     RVNTVQDFLERPLVLENPSSYITFAADTLPEWEFLSRLAEETGCLLLLDVNNVYVSSR
                     NHDFDPHEYLRSIPHDRVQQFHLAGHTDLGTHCIDTHDGRVVDRVWELYREAVGLTGG
                     AATLLEWDARIPSFEEVHTEALKARRFVAEGTGDREQGTGGVELVPSIEYSVRSTQYS
                     ESVPHPSHLITAEAE"
     gene            701..1480
                     /locus_tag="VFV87_06610"
     CDS             701..1480
                     /locus_tag="VFV87_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX5103463.1"
                     /translation="MNVSVQSLGELQHWLLDAITLPEDADPAGVAAAIVPSRNQSSAE
                     RLAVYRHAYLARLLEVLRELFPCTRFAVGDELFDQFAAGYLQQYPPHSYTLARLADRW
                     VEHLDETRPANAEWGRFIVELSRLEQAIDRIFDGPGPENLPPFTLPANASGDLQLSFV
                     PGFELNAFQFPISDFYTAWKAGEHPSWPTPREQFLALFRRDHIVRRHELSREQYALLS
                     AIQRGQTLGRSLGELRDYTPERIRRWFTNWSASRFFASVPG"
     gene            complement(1466..2203)
                     /locus_tag="VFV87_06615"
     CDS             complement(1466..2203)
                     /locus_tag="VFV87_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103464.1"
                     /translation="MFLTRLRWTAVIDPYDPLDAIEVVSTDGRAICYLAQSAGNCHFL
                     LFVDESPDEECLLRRDNVAENILLRVPSGRLVATGFEELCGDEEDRYAPDRFEPTKGE
                     TVRIPVGNYRVTACELNWNDLVKREISRRAVPGDERFETIVGSSMGCVTVSTIFLAPT
                     GLLIVWLEFGSQTALRAVGYVLLFQLVFWALAIALICFSKAWGRLNRLRDEIKSEFPD
                     ALLVLRTLGHDEGGASFNTGIFGSAWD"
     gene            complement(2300..>2856)
                     /locus_tag="VFV87_06620"
     CDS             complement(2300..>2856)
                     /locus_tag="VFV87_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5103465.1"
                     /translation="QTAGVDFLAADGHKWLLGPEGAGILFVKHEHLDRLRPLCVGWNS
                     VVQGNDYTKIELNIRPAAARYEGGSQNMVGFIGLGASLDLLASLGVGPQASPVADHVL
                     ALTDHACERLLSLGATLLAPRTGPHRSGIVTFQLPGRDANEIRRHLESAHIITRCRAG
                     GVRISPHGYNTTDEVDRMISELKP"
BASE COUNT          535 a          909 c          870 g          542 t
ORIGIN      
        1 tcaaacggct ggcgggcgag gtcaacgctc gctggatttc ggatcacttg tgctggacgg
       61 gggtggccgg acgcacgacg cacgacctgc tgccgttgcc gctgaatgag gaagcgttgt
      121 cccatgtggt cggccgagta aacacggtac aggacttcct cgagcggccc ctcgtcctcg
      181 aaaacccgtc gagctacatc acattcgccg ccgatacgct gcccgagtgg gaattcctct
      241 cgcggctggc cgaagaaacc ggctgcctgt tgctgctgga tgtgaacaac gtctatgtca
      301 gcagccgcaa tcacgatttc gatccgcacg aatacctgcg gtcgattccg cacgatcgcg
      361 tgcagcagtt ccacctggcc gggcataccg atctcggcac acactgcatc gacacgcatg
      421 atggccgcgt ggtggatcgc gtctgggagc tttaccgcga agcggtcggc ttgaccggcg
      481 gcgcggcgac gctgctggag tgggatgcgc ggattccgag ctttgaggaa gtccataccg
      541 aggcgctgaa ggcgcgtcgg tttgtagcgg aggggacagg ggacagggaa caggggacag
      601 ggggcgtcga actggttccg agtattgagt actcagtgcg aagtactcag tactcagaat
      661 cggtcccaca tcccagccac ctcatcacgg cggaggccga atgaacgtct ccgtccagtc
      721 gctcggcgaa ctccagcact ggctgctcga tgcgatcacg ctgccggagg atgcagatcc
      781 ggccggagtc gcggcggcga ttgtccccag ccgtaatcaa tcgtctgccg agcggctggc
      841 cgtctatcgg cacgcttacc tggcccggct gctggaggtg ctgcgcgagc tgtttccctg
      901 cacgcggttc gctgtcggcg acgagttgtt cgaccaattc gccgccggct acctgcagca
      961 atatccgccg cacagctata cgctcgcccg gttggccgac cggtgggtcg agcatctcga
     1021 cgaaacccgc cccgccaatg ccgagtgggg ccggttcatc gtcgagctct cgcgcctgga
     1081 gcaggcgatc gaccgcatct tcgacggccc cggccccgaa aacctgcccc ccttcacgct
     1141 tcctgccaat gccagcggcg acctgcagct ctcgttcgtc cccggcttcg agctgaacgc
     1201 tttccagttc ccaatcagcg acttctacac cgcctggaaa gccggcgagc atccatcgtg
     1261 gccgaccccg cgagagcagt tcctcgccct cttccgccgc gaccacatcg tccgccggca
     1321 tgagctgagc cgcgagcagt acgcgttgct gtctgccatc cagcgggggc aaacgctcgg
     1381 gcggtcgctt ggggaactcc gtgattacac gcctgaacgc attcgccggt ggttcacgaa
     1441 ctggtcggct tcacggttct ttgcttcagt cccaggctga gccgaatatc cccgtattga
     1501 acgaagcgcc gccttcatcg tggccgagtg ttcgcaatac cagaagcgca tctggaaatt
     1561 ccgacttgat ttcatccctg agtcgattca ggcggcccca ggctttcgaa aagcagatga
     1621 gtgcaatggc caacgcccaa aacacaagtt gaaacaacag gacgtagcca accgcgcgca
     1681 gtgcagtttg agaaccgaat tccagccaaa cgatcagcaa tcctgttggc gccaaaaaga
     1741 tggtagatac ggtaacacac cccatactcg agccgacgat cgtctcaaat cgctcgtccc
     1801 ccggaacggc gcgccgtgat atttctcgtt tgaccaggtc attccagttc aactcgcacg
     1861 ccgtcaccct gtagtttccg actgggattc tcaccgtttc gcccttggtc ggctcgaacc
     1921 gatcaggagc gtaccggtct tcttcgtcgc cgcatagctc ctcgaatccc gtagcgacca
     1981 acctgccgct tggaacacgc agaaggatgt tttcggcaac gttgtcgcgg cgcaggagac
     2041 actcttcgtc gggagattca tcgacaaaga gcaagaaatg acagttcccg gccgactgcg
     2101 cgaggtaaca aattgcccgc ccatcggtgc tgacgacttc gatagcgtcc agcgggtcgt
     2161 aaggatcgat aaccgctgtc caccgcagcc gggtcaaaaa caatgtacat cgccacatcc
     2221 gtcgttgcac gtagtcgtat gcgcatgtgc ggagtattgt gctattggaa tccggattgt
     2281 ttctcgcgga ccgaaacgcc tacggtttca gctcgctgat catccggtcc acttcgtccg
     2341 tcgtgttgta cccgtgcggg ctgatacgga cgccgccggc tcggcagcgg gtgatgatgt
     2401 gggccgattc gaggtggcgg cggatttcgt tggcgtcgcg gccggggagt tggaaggtga
     2461 cgataccgga gcggtgcggg cctgtgcgcg gggcgagcag cgtcgcgccg agagagagca
     2521 gccgctcgca ggcgtggtcg gtcagggcca gcacatgatc ggcaacgggc gacgcttgcg
     2581 gcccgacgcc gaggctcgcc agcagatcga ggctcgcacc caggccgatg aagccgacca
     2641 tgttctgcga gccgccctcg taccgcgccg cggccgggcg gatgttcagc tcgatcttcg
     2701 tgtaatcgtt tccttgcacc acgctgttcc agccgacgca gagcggccgc aaccgatcga
     2761 gatgctcgtg cttgacgaac aggatgcccg cgccctccgg gccgagcagc cacttgtgcc
     2821 catcggctgc caggaaatcg acgcccgcgg tctgta
//
LOCUS       DASUGB010000344         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_39048,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000344 DASUGB010000000
VERSION     DASUGB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39048"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1033
                     /locus_tag="VFV87_06625"
     CDS             155..1033
                     /locus_tag="VFV87_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HEX5103466.1"
                     /translation="MLATIEAPRAARWTEADLQEALARYPFYHIIRLNESLTTPGNPA
                     FLPAQRLCLKQLKSLDLTGKRVLDIGCRDGLYSFAAESLGAAEVIGIDNDLSPAATEF
                     LIPYFDSQVRMHQMNVYDLTRQTFGQFDVVIFPGVLYHLRYPFWALKAIRDVLRVGGH
                     LIVETAIWEGEPNNAVLFCPIEQDSPYEVSSCTFFNPKGLVDSLQSLGFETTHWELLK
                     RPTLAARLGQHARRAADWLRSCFWSVDRGPIRRVRRGVFHSVYRGFPKDTILGRYWEG
                     THDYHSGLMRSVECVR"
     gene            1286..2128
                     /locus_tag="VFV87_06630"
     CDS             1286..2128
                     /locus_tag="VFV87_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HEX5103467.1"
                     /translation="MQTALSGLHAATASLQVAANNFANQQTPGFKASRILLADQGPAT
                     LAYSAAGAAQLIQVGRGVSVAGIDVNFAPGATQPGDDPTALLSLQGEGLFILEGERGE
                     RLYTRDGHFRLNANGELVTSQGRRVLGYAADPRGEIDTSELVELRIVIGSQAPAEGGQ
                     SAELQNYSIDPRGRIVGRYSDGSRRPLGQLRLARFPNPSGLTQRGGNAYAATPASGLP
                     IESNPGEGGAATVLNGTTELSNVDLGRELIELTLAGTQFRANLTVFRTADALLGELFF
                     PWRR"
     gene            complement(2340..3248)
                     /locus_tag="VFV87_06635"
     CDS             complement(2340..3248)
                     /locus_tag="VFV87_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5103468.1"
                     /translation="MLRIAIAVCCLLSLPLSCLAQEKAAQQKAAEPKVPEGVVYEKDV
                     QHGTGGGEPLLLDIARPEKATKAPCIVVIHGGGWRGGNFKVHVPHILEFAKRGYVSAT
                     VQYRLVPKAAWPAQIEDVKCAVRYLRANADKYGIDKDRIGAIGFSAGAHLSMLLGTMD
                     KADGLEGEGGNPNESSKAQAVVAYFGPTDLTQDDYPDVVKQMINDLVGGKVADKLDVA
                     KSASPITYVDSADAPTLIYQGTKDRLVPYTQAVLMADAMTKAGVPGRVELLLGADHGW
                     GGPEILRTGEDSIGFFNLHLKHTPAK"
     gene            complement(3257..>3578)
                     /locus_tag="VFV87_06640"
     CDS             complement(3257..>3578)
                     /locus_tag="VFV87_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis bifunctional protein
                     CbiET"
                     /protein_id="HEX5103469.1"
                     /translation="LGKAPEAWAGLPQPDAVFVGGTGRQVRRIVELAFEQLRAGGRLV
                     ANVGSIENLAAVREVLLAKGGDPSVLMINLARGNDQMDRLNFEALHPTFLVSAVKPSV
                     SPVY"
BASE COUNT          619 a         1147 c         1146 g          666 t
ORIGIN      
        1 ccgtgacctg acggtcacgg ctacttcctg ccggccctcc gggcctcgaa ccgcatggat
       61 catcgcatgc tcctctataa ctgcacttgc cccagccgct tgccagtgcg taccctcccg
      121 cctgaaccgt accgtcactc cgctgggcca cgccatgcta gcaactatcg aagcgccgcg
      181 cgccgcgcgc tggactgaag ccgacctcca ggaggcgctg gcccgctacc cgttctatca
      241 catcatccgc ctcaacgaat cgctcacaac gcccggcaat cccgccttcc ttcccgccca
      301 gcggctgtgc ctcaagcagc tcaaatcgct cgacctgacc ggcaagcggg tcctcgacat
      361 cggctgccgc gacgggctgt actccttcgc cgccgaatcg ctgggggccg cggaagtcat
      421 cgggatcgac aacgatctct cgccggcggc cacggaattc ctgatcccgt atttcgattc
      481 gcaggtgcgg atgcaccaaa tgaacgtcta tgacctcacg cgccagacct tcggccaatt
      541 cgacgtggtg atcttccccg gcgtgctcta tcacctccgc tatccgtttt gggccctcaa
      601 agcgatccgc gacgtgctcc gcgtcggcgg ccacttgatc gtcgaaacgg ccatctggga
      661 gggcgagccg aacaacgcgg tattgttctg ccccatcgag caggacagcc cctacgaagt
      721 cagcagttgc acgttcttca atcccaaggg gctggtcgat tcactccagt cgctcggctt
      781 tgagacgacg cactgggaac tgctcaaaag gccgacgctg gctgcccgcc tgggccagca
      841 cgcccgccgc gccgccgact ggctgcggag ctgcttctgg tcggtcgatc gcgggcccat
      901 ccgccgcgtc aggcggggcg tctttcacag cgtttatcgc ggcttcccca aggacacaat
      961 tcttggcagg tattgggaag ggacgcacga ctatcacagc ggcctgatgc ggtctgtaga
     1021 gtgcgtgcgg tagcgcgatg ctgtacgggg caattgagaa ttgcaaaatg aaaagttcaa
     1081 attgcaaagt gtagtcttca attcagaggt ttcattttgc aattcgcaat tcgcaatttg
     1141 caatcgatgg gcaagcacgc acgtaattgt gtccgctctt cacgcgcgcc gtcgcccaga
     1201 gactggattt tatgagctag atttgtgatg ggcttttcgg tccccgctcg cttggcggag
     1261 accggttatg gggctgggga cggtcatgca gacggccctt tcgggcctgc acgcggctac
     1321 ggccagcctg caggtcgcgg cgaacaattt cgcgaatcag cagacacccg gcttcaaggc
     1381 ttcgcggatt ctgttggccg atcaagggcc ggccacgctc gcctattctg ccgcgggcgc
     1441 agcgcagttg atccaggtcg gccgcggcgt cagcgtggcc gggatcgacg tcaattttgc
     1501 gccgggcgcc acgcagccgg gcgatgatcc aaccgccctg ttgtcgctgc agggcgaggg
     1561 gctgttcatt ctggaaggag agcggggcga gcggctctac acgcgcgacg gccacttccg
     1621 ccttaatgcc aacggcgagc tggtgacgag ccagggccgg cgcgtcctgg gctatgcggc
     1681 agatccgcgg ggcgagatcg acaccagcga gctggtggaa ctgcggatcg tgatcggttc
     1741 gcaagcgccg gcggagggcg gccaatcggc ggagctgcag aactactcga tcgatccccg
     1801 cggccggatc gtcggccgct atagcgacgg ctcgcgtcgg ccgcttgggc agttgcggct
     1861 ggcccggttt cccaacccca gcggcctgac gcagcgcggc ggcaatgcct acgccgcgac
     1921 gcccgcttct ggcctgccga tcgaaagcaa tcccggcgaa gggggagccg ccaccgttct
     1981 caacggcacg accgagcttt ccaacgtcga tctcggccgc gagctcatcg agctgacgct
     2041 cgccggtacg caattccgcg ccaatctcac cgtcttccgc accgccgacg ccctcctcgg
     2101 cgaactgttc tttccttggc ggcgataggt ggagtttggc gacaagttgc tgcggaagac
     2161 cgaattagcg atgacgaacg aggcgttggc cagtctactc taccatcatg accgatgtct
     2221 ggcttaaggg gtgtcgagct gggagaacgg cacaagcgcc ctaccgctgc ggataagcat
     2281 cggcacgatc ggcggaagtg gggaaatggg ctgttgaaat cagcagctgc ttctcccgcc
     2341 tacttggccg gcgtgtgctt caggtgcagg ttgaagaacc cgatcgagtc ttcgccggtg
     2401 cggaggattt ccgggccgcc ccagccgtgg tcggctccca gcaatagttc gacgcggccg
     2461 ggcacgccag ctttggtcat ggcgtcggcc atgaggacgg cttgggtata gggaacgagg
     2521 cggtccttgg tgccttgata gatgagggtc ggggcgtcgg ccgaatcgac ataggtaatc
     2581 ggcgaggcgg atttggccac gtcgagctta tcggccactt tgccgccgac caggtcgtta
     2641 atcatctgct tgacgacgtc gggataatcg tcctgcgtca ggtcggtcgg gccgaaataa
     2701 gcgacgacgg cctgcgcctt gctcgactcg ttcggattgc ccccttcgcc ttccaggccg
     2761 tcggctttgt ccatcgtgcc cagaagcatc gagaggtggg cgccggctga gaagccgatc
     2821 gcgccgatgc ggtctttgtc gatgccgtac ttgtcggcat tggcccgcag ataacggacg
     2881 gcgcacttca cgtcctcaat ctgggccggc caggccgcct tgggcacaag gcggtattgc
     2941 accgtcgcgg aaacgtagcc gcgcttggcg aattccagaa tgtgcggcac gtggaccttg
     3001 aaattgccgc cccgccagcc gccgccgtgg atgacgacga tgcagggcgc ctttgtcgcc
     3061 ttctcgggac gggcgatatc gagcagcaaa ggctcgccgc cgccggtgcc gtgctgcacg
     3121 tccttctcat agacgacgcc ctcggggacc ttgggctcgg cagccttctg ctgggctgct
     3181 ttctcttggg ccaggcagga aagggggagg gacaacaggc agcatacagc gatcgcgatg
     3241 cgcagcatgg gtgacctcaa tagacggggg agaccgaagg cttgaccgcg gaaacgagga
     3301 aggtggggtg cagggcctcg aaattcaggc ggtccatctg gtcgttgccg cgggccaggt
     3361 tgatcatcag caccgacgga tcgcccccct tggcgagcaa tacctcgcgg accgccgcca
     3421 ggttttcaat gctgccgaca ttggcgacca gccgcccgcc cgcgcggagc tgctcgaagg
     3481 ccaattcgac gatccgcctc acctggcggc cggtgccgcc gacgaagacg gcgtcgggct
     3541 gcggcaggcc ggcccaggct tcgggggcct tgccgagg
//
LOCUS       DASUGB010000345         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_39117,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000345 DASUGB010000000
VERSION     DASUGB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39117"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFV87_06645"
     CDS             <1..992
                     /locus_tag="VFV87_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX5103470.1"
                     /translation="TAGIDRTKKELSRDLAYLLRLWKVIVRRIKKYPAPIDIYEESDM
                     IIRTIRDIFTGDVDAIYIDEPQAYNRAKEFLELVMPRHVDRLHHYEGREPLFHKYKLE
                     NEIAQIYQRKVPLRGGGSIVIDQTEALVAIDVNSGSFRAEGDAEETAYQLNLSAAKEI
                     ARQLRLRDLGGVIVNDFIDMRRERHRRNLERALRDAMKRDRARTKILRTSPFGLIEMT
                     RQRIRPSLKRSVYGDCPCCGGRGVTKTPESMAIEVVRMLALASHQPKIARVTVRVNDE
                     VATYLNNKKRREIMQLEDAARMLVQILGSEGHFPEHLELECRDTDDREITLPQ"
     gene            complement(1153..1404)
                     /locus_tag="VFV87_06650"
     CDS             complement(1153..1404)
                     /locus_tag="VFV87_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103471.1"
                     /translation="MLLLSEAGHLTGLGELLAIGALFILLVIVAVYVAIIAATVLVLK
                     RLLQSRSRGVQIAAWLLTAATFTVPVLFGMIFLRELVNG"
     gene            1817..2302
                     /locus_tag="VFV87_06655"
     CDS             1817..2302
                     /locus_tag="VFV87_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103472.1"
                     /translation="MSFARKSALVLVVVLGRTAAGQHEMIPAPLGDVVRTAGEVSLDP
                     AAPSLELGFRKTVFRNDYAHFQFYPGFATAYFTLWHRASDPNTGCTVLIKRTYLSRML
                     AVTIDDPHKWIWANTDPLPHQIEWGFSKQSCDGDYFAIYYRRPGGPWQLYGLFERDYP
                     N"
     gene            2879..3928
                     /locus_tag="VFV87_06660"
     CDS             2879..3928
                     /locus_tag="VFV87_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5103473.1"
                     /translation="MSKIFISYRRQDSRDVTGRIYDRLLRHFEAERIFKDVDNIAPGE
                     DYRDAIDREIGACSVLLAIIGPTWLKAKKGDGKLRLDDPHDMVRIELETALRREKRVI
                     PILVTDARMPDSDELPDTLKELADRNALAVRHDPDFHRDMDRLVELMERAMTSNAPVL
                     AELIDPNWANAVVPPPVQPAHFDGPHAVSRPAGFEASPHYGQAEVSPFASPSGSYPRP
                     GSASPEGMITPAAVVLGTICLLGLAFCSLMLIGAFASYSDPEGVDKEMDLVLGAACAV
                     GWLGDLIGLAAAAGMFFRKLYWPVVLGVCAMALCTLVFCPVLNLPGAAFALYVLFRPG
                     VRGVFEEKTGGAVIA"
     gene            complement(3937..5559)
                     /gene="serA"
                     /locus_tag="VFV87_06665"
     CDS             complement(3937..5559)
                     /gene="serA"
                     /locus_tag="VFV87_06665"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5103474.1"
                     /translation="MPRIIILDPIAQEGLDLLKHAGIDHEVRTGLKGEALKKALAEFD
                     GAICRSGVTISGDVLEGNKRLRAIARAGVGTDNIDKEAATRLGIVVMNTPTGNTLSTA
                     EHAFTLMLALSRRVAEAYATLCKGKWDRKTFMGTQLADKTLGIVGLGRIGQEVAKRAL
                     AFQMRVLGYDPFLSAEQAAKLGITRVEKVRDMLPQVDYLTVHTPLTPETTNLIDCEAI
                     ALMKPGVRLINAARGGIYNEEALVEGLKGGRIGGVALDVFVEEPNTSSPLFGMPNVLC
                     TPHLGASTEEAQTQVAVEAVELLINYFTKGEVRHAVNMAAIDPKTLAAIRGYLDLAHR
                     LGLLMSQWHSSGASACHVTFRGELAKKNTKLVTAAFCAGLLERALEEDVNIVNAELLL
                     RERGIQLTEESRSEPGAFSSALAVEVTSGNGEKFGAAGTLFGNDMPRLIRLGDFRLEA
                     YLDGILLVFTHHDVPGIIGKVGSIFGNHRVNIAQMAVGRTAPGGEAIGVLNLDALPPK
                     ASVDEVAGHADITGIRVIELPTAGKLPTWLRA"
     gene            complement(5563..5949)
                     /locus_tag="VFV87_06670"
     CDS             complement(5563..5949)
                     /locus_tag="VFV87_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5103475.1"
                     /translation="MPMTEQQFKDRTKSVGLRIIRLVEALPPAKGARVIGDQLLRSGT
                     SIGANYRASCRAISVPDILSMLGDVEEETGESMYWIEMLGDAGIVPQRRLASLHRELG
                     EILAMIVASIKTLRRRNPKSKIQNPK"
BASE COUNT         1107 a         1938 c         1943 g         1135 t
ORIGIN      
        1 gcacggccgg catcgaccgg acgaagaaag aactgtcgcg cgacctggcg tatctgctgc
       61 gcctgtggaa ggtcatcgtc cgccggatca agaagtaccc ggcgccgatc gacatctacg
      121 aagaaagcga catgatcatc cgcacgatcc gcgacatctt caccggcgac gtcgacgcga
      181 tctacatcga cgagccgcag gcctacaacc gggccaagga gtttctggag ctcgtgatgc
      241 cgcggcacgt cgatcggctg caccactacg aggggcgcga gccgctgttc cacaagtaca
      301 agctggagaa cgagatcgcc cagatttacc agcggaaggt gccgctccgc gggggcgggt
      361 cgatcgtcat cgaccagacc gaggccctgg tggccatcga cgtcaatagc ggcagctttc
      421 gggccgaggg ggacgccgag gagaccgcct atcagctcaa cctctcggct gcgaaggaga
      481 tcgcccggca gcttcgcttg cgcgatctgg ggggcgtgat cgtcaacgac ttcatcgaca
      541 tgcgccgcga gcggcaccgc cgcaacctgg agcgggcgct gcgcgacgcg atgaagcgcg
      601 accgggcccg cacgaaaatc ctccgcacca gcccgttcgg gctgatcgag atgacccgcc
      661 agcggattcg ccccagctta aagcggagcg tctatggcga ttgcccctgc tgcggcggcc
      721 gcggcgtgac caaaacgccc gagagcatgg cgatcgaggt cgtccgcatg ctggccctcg
      781 ccagccatca gccgaaaatc gcccgcgtga ccgtccgggt gaacgacgag gtggcgacgt
      841 atctcaataa caagaagcgt cgcgagatca tgcagctcga agacgccgcc cggatgctcg
      901 tccaaatcct cggcagcgaa ggccacttcc ccgagcacct cgaactcgaa tgccgcgaca
      961 ccgacgaccg cgaaatcacg ctgccgcagt agagtagcac gccagcttcg tgtgccgtcc
     1021 gcgatgagcc gcgaagcggc ggcaggtatt tgccatgggc gtcagcccac gggaccctac
     1081 cctcattgaa ttccccagcc cggaaggggc ggcaccgagg gcgcccgtgc gcctgcggct
     1141 ctccaacgaa ctctagccgt tcacaagctc cctcaggaag atcatcccga acagaactgg
     1201 cacagtgaac gttgctgccg tcagcagcca ggcggcaatc tgcactccgc gtgagcgcga
     1261 ctgcaggagt cgcttgagaa ccagcaccgt tgccgcaatg attgcgacgt acaccgcgac
     1321 gatcacaagt aaaataaaga gcgcgccgat tgcgaggagt tcgccaaggc ccgtgaggtg
     1381 acccgcttcg gaaagcaata acatgacctg gatcccctat tacgtcggcg taggccatca
     1441 tagtggccgg aggcggatag tgtcctgacc agtgcattga tcggagagcc gagcccagga
     1501 tcggtgggca acctgaaccg gactcggtcg gcttgctcgc gtcaagcccg cggcttcagc
     1561 ccgggttggc gcgccccccc gcgcgcaacg attgtacggc agccggcttt catcggcggc
     1621 ttaagccctc gcgaagaagc cggctgaacg gacagtccct cacaggcggc ctacgcgttt
     1681 cagtcgttgc agccgatcca aaccgcagct ttccacgtaa cgggggcctc catcgatgcc
     1741 tccctgtgat tgccaaatcc caattgcgag ggagatttct ctgcaccgac agcttcgatc
     1801 tctcggagac gctcccatgt cgttcgcacg aaagtccgct ctggtattgg tcgtcgtgct
     1861 tggtcgcact gcggctggcc agcacgagat gatccctgcg ccactcggcg acgtggttcg
     1921 gacggcaggc gaggtctcac tcgatcccgc cgcaccttcc ctggaactgg gattcaggaa
     1981 gacggtgttt cgaaacgact acgcacactt tcaattttat ccgggctttg cgacggccta
     2041 ttttacgctg tggcaccggg cctcggaccc caacacggga tgcaccgtgc tgatcaagcg
     2101 cacgtatctt tcccgaatgc tcgcagtaac gatcgatgat ccgcacaagt ggatctgggc
     2161 taacaccgac ccgctccccc atcaaatcga atgggggttt agcaagcagt cctgcgacgg
     2221 cgactacttc gcaatttact atcgccgccc aggcggtccc tggcaactgt acggactgtt
     2281 cgagcgagac tacccgaact gacgggcaga acaccaatct tgaacccgcg gtagcggaca
     2341 agagcccacg gaacctggtc taaggaattc gccggtcccg aaggggcggc gccgagtcgc
     2401 gccggcaccg ctcccatgcc gccgctgcgc ggctgaatgc cactttctgc gacgccgacc
     2461 gtgggctcac ccccacagct caacgattcc gccgctgccg cggcaccgat cgcagcaacc
     2521 ggccatgtca gcccaaaggg cgaggcatcc tgcctgctgg agcctgtccc tttgccgtcc
     2581 tggggtgctc tgcgccaatt ggcgatcctg cggtcggcca agccgaacga ccaggtggtt
     2641 cctgaacctg acgcaggcca tctaggaatc agatgctgcg gcaggctcaa ccggggcggt
     2701 agggtcgccc ggctggaagg ggtcgggagt caattgcggg aaccgttctc catcagccaa
     2761 cgtcgtagcc gcaattgact cccgactccc ttggggcgca cccgctttcc gcgctgcgtt
     2821 ccttgggacg tttggctaga atgggcacgg ccgaacgaca tcttactgcg cgcttcgcat
     2881 gtccaagatt tttatctcct atcgccgaca ggacagccgc gatgtgaccg gccggatcta
     2941 cgaccgtctg ctgcggcact ttgaggcgga gcggatcttc aaggacgtcg ataacatcgc
     3001 gcccggcgag gactaccgcg acgcgattga ccgcgaaatc ggcgcttgct cggtgctcct
     3061 ggcgatcatc ggcccgacct ggctcaaagc gaaaaagggg gacgggaagc tgcggctgga
     3121 cgatccgcac gacatggtgc ggatcgaatt ggagaccgcc ctgcggcgcg agaagcgggt
     3181 gattcccatt ctggtgaccg atgcccgcat gccggattcc gacgagctgc ccgataccct
     3241 caaagagctg gccgatcgca acgccctggc cgtgcggcac gatccggact ttcaccgcga
     3301 catggaccgg ctggtggagt tgatggaacg ggcgatgacg agcaatgcgc ccgtgctggc
     3361 ggagctgatc gatccgaact gggccaacgc ggtcgtgccg ccccccgtgc agcccgctca
     3421 ctttgacggg ccgcacgccg tgtcccggcc cgcgggattt gaagcatcgc cgcactatgg
     3481 acaggcggag gtaagtccct ttgcctcgcc ttccggctcg tatccgcggc ccggcagcgc
     3541 gtcgccggaa gggatgatca cgccggcggc ggtcgtgctg gggacgattt gtctgctggg
     3601 gctggcgttc tgctcgctga tgttgatcgg cgctttcgcc tcgtacagtg accccgaagg
     3661 ggtggacaag gagatggacc tcgtcctggg ggcggcgtgc gcggtgggct ggttgggcga
     3721 cctgatcggc ctcgcggcag ccgcgggaat gtttttccgc aagctctact ggccggtggt
     3781 gcttggcgtg tgcgcgatgg cgctctgtac gctcgtgttc tgcccggtcc tgaatttgcc
     3841 gggcgcggcg tttgcgctgt acgtgctctt ccggcctggc gtgcgggggg tatttgagga
     3901 gaagaccgga ggggcggtta ttgcatagcg cccagatcag gcccgcagcc aggtgggcag
     3961 cttgcccgcg gtcgggagct cgatcacgcg gatgccggtg atgtcggcgt ggccggcgac
     4021 ttcgtccacg gaggctttgg gagggagggc gtcgaggttc aggacgccga tggcttcgcc
     4081 gccgggggcc gtacggccga cggccatctg ggcgatgttg acgcggtgat tgccgaagat
     4141 cgaccccact ttgccgatga ttccgggaac gtcgtggtgt gtgaacacga gcagaatgcc
     4201 gtcgagatag gcttccaggc ggaaatcgcc cagacggatc aaacgcggca tgtcattacc
     4261 gaagagcgtg cccgcggcgc cgaacttctc gccgttgccg ctggtgactt cgaccgcgag
     4321 agccgagctg aaggcgcccg gctcgctgcg cgactcctcg gtgagctgga tgccacgctc
     4381 gcggagcagg agctcggcgt tgacgatgtt gacgtcctct tcgagcgccc gctctagcag
     4441 gccggcacag aaagcggccg tgacgagctt ggtgttcttc tttgccagtt cgccgcggaa
     4501 cgtcacatga caagccgatg cgccgctcga atgccactgc gacatcagca ggcccagacg
     4561 atgggcaaga tccaggtagc cgcggatcgc ggcaagcgtt ttgggatcga tcgccgccat
     4621 attgaccgca tggcggactt cgcccttggt gaagtagttg attaggagct cgaccgcctc
     4681 gacggcgact tgcgtctggg cctcttcggt gctcgcgccc aggtgcggcg tgcagagcac
     4741 attgggcata ccgaagagcg ggctggacgt gtttggctct tccacaaaga cgtcgagggc
     4801 cacgccgccg atcctgccac ctttgaggcc ctcgacaagc gcctcctcgt tgtagattcc
     4861 gccgcgagcc gcgttgatca gccgcacgcc gggcttcatc agggcaatcg cttcgcagtc
     4921 gatcaaattc gtcgtttcgg gagtcagtgg cgtgtgtacg gtgaggtaat cgacctgcgg
     4981 cagcatgtcg cgaaccttct cgacccgcgt gatgccaagc ttggccgctt gttccgcgga
     5041 gaggaacggg tcgtagccga ggacgcgcat ctgaaaggcg agcgcccgct tggcgacttc
     5101 ctggccaatg cgccccaggc cgacaatgcc gagcgtcttg tcggcgagct gggtccccat
     5161 gaatgttttg cggtcccact ttcctttgca aagcgtagcg taggcttcgg ccacgcggcg
     5221 gctgagggcg agcatcaacg tgaaggcatg ctcggccgtg ctgagcgtgt tgccggtggg
     5281 cgtgttcatc accacgatgc cgaggcgcgt ggcggcttcc ttgtcgatgt tgtcggtgcc
     5341 gaccccggcg cgggcgatcg cccgcaaacg cttgttccct tccagcacat cgccgctgat
     5401 cgtcacgccg ctgcggcaga tcgcgccgtc gaactccgcc agcgccttct tcagtgcctc
     5461 gcccttaagt cccgtgcgga cttcgtggtc aatgcccgcg tgctttagca ggtcgagccc
     5521 ttcctgggcg atggggtcga ggatgatgat gcgaggcatg ggctattttg gattttggat
     5581 tttggatttt ggattgcggc ggcggagcgt cttgatcgag gcaacgatca tggcgaggat
     5641 ctcgcccagc tctctgtgaa ggcttgccaa gcggcgctgc ggcacgattc ccgcatcgcc
     5701 gagcatttca atccagtaca tgctctcgcc ggtttcttct tccacgtcgc cgagcatgga
     5761 gagaatatcc gggaccgaga tggcacggca cgaagcgcga tagttcgctc cgatcgacgt
     5821 tccgcttcgc agcaactggt cgccgatcac tcgggcgccc tttgctggcg gaagcgcctc
     5881 gaccagtcgg atgattcgca agcccacgga tttcgtccga tccttgaact gctgttcggt
     5941 cattggcatt tcggcattcc gattgaggaa gtgaatggga ccatcggacg cccgtccttt
     6001 gtatccaatc caaaatccaa aatccaaaat ccaaaatttg cggcgctgct tatccccgct
     6061 tgtctttgaa ttcccgcatg aacgctgcca actgctccac cccctccagc ggcatcgcgt
     6121 tat
//
LOCUS       DASUGB010000346         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1845708, whole genome shotgun sequence.
ACCESSION   DASUGB010000346 DASUGB010000000
VERSION     DASUGB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845708"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1110
                     /gene="lpxA"
                     /locus_tag="VFV87_06675"
     CDS             250..1110
                     /gene="lpxA"
                     /locus_tag="VFV87_06675"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX5103476.1"
                     /translation="MTQIHPTAIVDPRAEIGQDVVIGPYCIIEAGAVIGDGCRLEARA
                     IVKSRTTLGKNNEIGEGAVLGGRAQHVQVQEPGGTLVIGDGNRIRENATIHRAWGNDS
                     VTTVGNGCMIMINAHIGHDSRIGNHCILVNNVMIAGHVTLEDRAYLAGASAVHQFTRV
                     GKLAMVGGMAKIKQDVPPFVLIDGSTSAEVVGLNKVGLRRSGCTAEDMFQLKAAYRVI
                     YREGRRWSEVLEILQTDFASGPAAAFYEFLKAGKRGFVQERRISRKATLKIVDASEDE
                     IGEDADRDAA"
     gene            1304..>2030
                     /locus_tag="VFV87_06680"
     CDS             1304..>2030
                     /locus_tag="VFV87_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5103477.1"
                     /translation="MVCFLLTLLPGAIRAADEGQRPNILFIYTDDQSYKTVRCYPDSY
                     PWARTPNIDALAKAGVRFERAYLGSWCMPSRATLLTGLYPHAIQSMRMEGEYPGSTYD
                     PAQCPFWPANFRKQGYQTAQIGKWHTGVDAGARRDWDFQIVWNRPKHPDNAGNYYYDQ
                     ILSTNGGPDTLTEGYSTDNYTKWACEYIRGEHRDQDKPWYLWLCYGGVHGPSIPAMRH
                     KGGYKDAEVVPPADIFPPRPGKNA"
BASE COUNT          406 a          666 c          608 g          350 t
ORIGIN      
        1 caagtcgtcg caggcggggt gcagcaggcg cgaagcgacc ttcatcgtaa atcatgccgc
       61 ccgttgcggc aaacgcctgt ggggcagaca ttcctgtctg ccgataggca aggatgcctg
      121 ccccacgtcg ccaaaaccga actcgacaag cccggcagaa tcgccataat tcccgcccgg
      181 ctccgcgtgc ctgaccggca ccgcagacaa gcagatagga agtctgccgc acaacacgaa
      241 agggatctcg tgacccagat tcatcccacc gccattgtcg atccgcgcgc cgaaattgga
      301 caagacgtcg tcattggtcc ctactgcatc atcgaggccg gcgccgtgat cggcgacggc
      361 tgccggctgg aagcacgggc catcgtgaag agccgcacga cgctgggaaa gaacaacgaa
      421 atcggcgaag gggcagtgct cggcggccgg gcccagcacg tgcaggtcca ggagccgggc
      481 ggcacgctgg tcatcggcga cggcaaccgc attcgcgaga acgccacgat ccaccgcgcc
      541 tggggcaatg actccgtaac gaccgtcggc aacggctgca tgatcatgat caacgcccac
      601 atcgggcacg attcgcggat cggcaatcac tgcattctcg tgaacaacgt gatgatcgcc
      661 ggccatgtga cgctggagga ccgggcgtat ctggccggag cttcagccgt gcatcaattc
      721 acgcgcgtcg gcaaactggc aatggttggc ggcatggcca agattaagca ggacgtgccc
      781 cccttcgtgt tgatcgacgg ctcaacctcc gccgaggtcg tcggactcaa taaagttggc
      841 ctgcgccgca gcggctgcac ggccgaagac atgttccagc tcaaggccgc ttatcgcgtg
      901 atctatcgcg aaggccgccg ctggagcgaa gtgctggaga tcctgcagac ggactttgcc
      961 agcggccccg ccgccgcttt ctacgagttc ctcaaggccg gcaaacgcgg cttcgtgcaa
     1021 gagcggcgca tctcgcgcaa ggcaacgctc aagatcgtcg atgcatccga agacgaaatc
     1081 ggcgaagacg ccgaccgcga cgcggcctga cgtggggcag acattcctgt ctgccggcgg
     1141 acaggaatgt ctgccccact tgccagctcg ctgattgcaa ttcggctttg cgtctgccat
     1201 cctacgcgct gggccgattc ccgctgcaat tcgtctcttc cgcgagtcgc caatgacttt
     1261 cctccctcgc tgcacgctgc atttgtgtct tttcgtgcct tccttggttt gcttcctcct
     1321 aactctgctg cccggcgcaa ttcgggccgc ggacgaaggc cagcggccaa acatcctctt
     1381 catctacacc gacgatcagt cgtacaagac ggtccgttgc tatccggatt cgtatccgtg
     1441 ggcgcgaacg ccaaacatcg atgccctcgc caaagccggc gtgcggttcg agcgggccta
     1501 tctcggctcg tggtgcatgc cttcgcgggc gaccctgttg accggactgt atccgcatgc
     1561 gatccagtcg atgcggatgg agggggagta tcccggcagc acatatgacc ccgcccaatg
     1621 cccgttctgg ccggcgaatt tccggaagca aggctaccag accgcccaga tcggcaagtg
     1681 gcacaccggg gtcgatgccg gggcgcgccg cgattgggat tttcagatcg tctggaaccg
     1741 gcccaagcat cccgataacg ccggcaacta ttactacgac cagatcctct cgaccaacgg
     1801 cggaccggac acgctgaccg agggctattc gacagacaac tacacgaagt gggcctgcga
     1861 gtacatccgc ggcgagcacc gtgaccagga caagccatgg tatctgtggc tctgctacgg
     1921 cggggtgcat ggcccctcca ttcccgcgat gcggcacaaa ggaggctata aagacgccga
     1981 ggtcgtgccg cccgccgaca tcttcccgcc gcggccgggc aaaaacgcct
//
LOCUS       DASUGB010000347         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1707462, whole genome shotgun sequence.
ACCESSION   DASUGB010000347 DASUGB010000000
VERSION     DASUGB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707462"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFV87_06685"
     CDS             <1..827
                     /locus_tag="VFV87_06685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5103478.1"
                     /translation="GLPALAQGASMARQLLLSASLTVFIFLGVPYALARLQGVSIAGG
                     FQIRKTGALSLAAAIILGISLAPLAYELIIASRELNIATLSDTQLRQFMHRLQEIVGQ
                     WRELHPLAILIPLAIVPAVCEEWFFRGYLLGALRGRAAAWFAIGATALIFGLFHASLG
                     GIIILERVLSSTALGLVLGWLCWTSRSVLPGMVLHALNNSLMLSLAYWGDGLKSLGLD
                     VENQQHLPLLWIAGAAALTAVGLLLAYLGRRPDASIAISSENPLKPLAAPPEPNAG"
     gene            complement(824..1204)
                     /locus_tag="VFV87_06690"
     CDS             complement(824..1204)
                     /locus_tag="VFV87_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103479.1"
                     /translation="MLRFEATVHSLRQPPVGLAALSVSFEAAYSHLAQLPRLFIEPDG
                     SFVWRGSDSNGDPWQVDGNLIDRGDALAFAEIKGCCPSERLDELLTALGWPEQRLVFQ
                     LPQRGVVLDEADFRRQAAAEAGAV"
     gene            1263..1859
                     /locus_tag="VFV87_06695"
     CDS             1263..1859
                     /locus_tag="VFV87_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103480.1"
                     /translation="MLSKVEIFSDASWERMIRAVEKVRERLGRASAALSQAGIPYAVA
                     GGNAVAAWVSTIDEAAVRNTQDVDIVLNRADFPSAIKALEAAGFAYRHAAGIDMFLDG
                     PGARARDAVHIVFAAEKVREEYLLPAPEVSESELQQNRSVLNLEALVRMKLTSFRDKD
                     KTHIRDLIGVGLIDSTWPDKFGNELAARLQHLLDTPDG"
     gene            1898..2218
                     /locus_tag="VFV87_06700"
     CDS             1898..2218
                     /locus_tag="VFV87_06700"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX5103481.1"
                     /translation="MSSSPAKPSAKPAAAHDDHPEVSAANARAGLWLFFVYLALYAGF
                     MGLTVLAPQAMGTPVLAGVNLAIAYGVGLIFAALVVAAIYMWICGRNARLAEQEAGLS
                     EGRP"
     gene            2215..>2783
                     /locus_tag="VFV87_06705"
     CDS             2215..>2783
                     /locus_tag="VFV87_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX5103482.1"
                     /translation="MIYETSWTAVGVFGFFVAVVLGISFYLGAKAKSAQGYYAAHGQI
                     PWFVNGVAFAGDYLSAASFLGICGMIAFYGYDGFLYSIGYLAGWIVALLVIAEPLKRL
                     GKFTFADALDSQFNSRGIKLAAAISTLAVSVFYLIPQMVGAGALIKPLLGMRDLPIGE
                     LTVSAHASGVILVGGIVILIVVTAGMVSTT"
BASE COUNT          460 a          870 c          879 g          574 t
ORIGIN      
        1 atggcttgcc ggcgctagca caaggcgcct ccatggcccg gcagctcctg ctgtccgcgt
       61 cgctgacggt gttcatcttc ctcggcgtgc cctatgcgct ggcccgattg cagggcgtgt
      121 cgatcgctgg cggctttcag atccgcaaaa caggcgcgct cagccttgct gcagcgatca
      181 tcctcggaat ctcgctcgcg ccgctcgcct acgaactgat catcgccagc cgggaactga
      241 acatcgcgac gctgagcgac acgcaactcc gccagttcat gcaccggctg caggaaatcg
      301 tcggtcaatg gcgggagctg cacccgctgg caattttgat tcctctggcg atcgttccgg
      361 cggtctgcga agagtggttc tttcgcggct acctgctcgg cgcgctccgc ggccgcgcag
      421 cggcctggtt cgcgatcggc gcgacggccc tgatcttcgg cttgtttcat gccagcctgg
      481 ggggcatcat catcctcgag cgcgtgttgt cgagcacagc gctgggcctg gtgctcggct
      541 ggctgtgctg gacaagcagg agcgttctgc caggaatggt tctgcacgcg ctcaataata
      601 gcctgatgct gtcgctggcc tattggggag atggactgaa atcactgggc ctggatgtag
      661 agaatcagca gcacttgccg ctgctctgga ttgccggagc ggcggcactt acggccgtcg
      721 gcctgttact ggcatacctt ggccgccggc cggacgcgtc cattgcgatt tcctcggaaa
      781 atccgctgaa accactggcc gctccgcccg agccaaacgc cggctagacg gcgccggctt
      841 cggccgccgc ctgtcgccga aagtccgctt cgtccagcac caccccgcgc tgcggcaatt
      901 gaaagacgag ccgctgctca ggccagccga gtgccgtgag cagttcgtcc agtcgttcgg
      961 acggacagca acccttgatt tcggcgaacg ctaacgcatc gccccggtcg atcaggttgc
     1021 catcgacctg ccacgggtcg ccattcgagt cgcttccccg ccagacgaac gagccgtcgg
     1081 gttcgataaa caaccgcggg agttgggcca ggtgcgagta tgccgcttca aacgacacag
     1141 acagcgccgc caggcccacc ggcggctgcc gtagcgaatg gacggtcgct tcaaaccgca
     1201 gcatgtcgtg agatccctcc gtatcagacg gggcggccgc gcctatagta gaatggccta
     1261 tcatgctttc caaggttgag atattcagcg acgcctcgtg ggaaaggatg atccgcgccg
     1321 tggaaaaggt tcgcgagcgc ctggggcgcg cctccgcggc gctttcgcag gcaggaattc
     1381 cctacgccgt ggctggcggc aacgctgtcg ccgcctgggt ctccacaatc gatgaggctg
     1441 ctgtccgcaa cacccaggac gtggacatcg tcctcaatcg ggcggatttt ccctcagcga
     1501 tcaaggcact ggaagcggcc ggtttcgcgt atagacatgc tgctggaatc gacatgtttc
     1561 tggacggacc gggcgcgaga gcccgtgatg cagtacacat cgtctttgcg gctgaaaagg
     1621 tccgcgagga atacctgttg cccgcgcccg aggtgagcga atcggagttg cagcagaacc
     1681 ggtccgtttt gaatttagag gcgctggttc ggatgaagct gacttcgttt cgtgacaaag
     1741 acaagacgca cattcgcgat cttatcggcg tcggccttat tgactcgacg tggccggaca
     1801 aattcggaaa cgagctggct gcccgcttgc agcacttact cgatacgccc gacggctgac
     1861 gtgacatccc gcggctgggc tgctattctg gcggcctatg tcgtcctctc ctgcaaagcc
     1921 atccgcgaag ccggcggcgg cgcacgatga ccatcccgag gtcagcgccg ccaatgcgcg
     1981 ggccggcttg tggctgttct tcgtttatct ggcgctgtat gcgggcttca tgggtctgac
     2041 cgtgcttgcc ccgcaagcga tgggcacgcc ggtgctcgcg ggagtcaatc tggcgatcgc
     2101 gtatggcgtc ggcctgatct tcgcggcgct cgtcgtcgcg gcgatctaca tgtggatctg
     2161 cggccgcaat gcccggctgg cggagcagga ggctgggctt tcggagggca ggccgtgatt
     2221 tacgagacat cctggacggc agtcggcgtc ttcggcttct ttgtcgccgt cgtgctgggc
     2281 atcagcttct acctgggcgc taaagcgaag tcggcgcagg gctactacgc cgctcacgga
     2341 cagattccgt ggttcgtcaa cggcgtggct tttgccggcg actatctctc cgccgcgtcg
     2401 ttcctcggca tctgcggaat gattgccttc tatggctacg acggcttttt gtattcgatc
     2461 ggctatctgg caggctggat tgtggccctg ctcgtcatcg ccgagccgct caagcgcctg
     2521 ggcaaattca cgttcgccga cgccctcgac agccagttca acagccgcgg gatcaagctg
     2581 gccgcggcaa tcagcacgct ggccgtaagc gtgttttatc tcattccgca gatggtcggc
     2641 gccggcgcgc tcattaagcc gctcctcggg atgcgcgacc tgccgatcgg cgaattgacg
     2701 gtctccgcgc atgcgtcggg agtcattctt gtcggtggaa ttgtgatcct catcgtcgtg
     2761 actgccggga tggtttcgac gac
//
LOCUS       DASUGB010000348         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1290543, whole genome shotgun sequence.
ACCESSION   DASUGB010000348 DASUGB010000000
VERSION     DASUGB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290543"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2291)
                     /gene="rpoC"
                     /locus_tag="VFV87_06710"
     CDS             complement(<1..>2291)
                     /gene="rpoC"
                     /locus_tag="VFV87_06710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5103483.1"
                     /translation="KDLAETNSKQKKKDLINRLKIVESIRDSDNRPEWMVLDVIPVIP
                     PDLRPLVLLDSGNFATSDLNDLYRRIINRNNRLRKLVDLNAPEVIIRNEKRMLQQSVD
                     ALFDNNRCKRPVLGSSNRPLKSLTDMIKGKQGRFRENLLGKRVDYSARSVIVVGPRLR
                     LHQCGLPKKIALELYQPFIIRKLKELGHADTIKSAKKMLERKDEEVWDILEAVIQNHP
                     VLLNRAPTLHRMGIMAFEPILVEGNAIHLHPLVCKGFNADFDGDQMAVHLPLSIEAQV
                     EAHTLLLSPHNIFAPANGKPIMSPSQDMVMGCNYITLEQPGRKGEGMAFTSPAEAELA
                     YSMGKIELHARIKVRLPSHQKLKSEGDEKHKPGTIIETTYGRILFNAILPRGMDFYNK
                     GMKSGDLAGVISDCYQSHGRRATIDLLDAMMQIGFREATRSGLSFATDDLLTPSTKVK
                     HISEAEKQVLRFKKLYERGVITEKERYNQVLDTWTHAREAITGEMREAMRTDVRGDSG
                     YVNPVYLMADSGARGGIEQIRQLAGMRGLMAKPTGEIIETPIKSNFREGLTVLEYFSS
                     THGARKGLADTALKTADSGYLTRKLADVAQNVVITATDCGTTQGITKGVIYRGEKVEV
                     KLSAAITGRVSRQSIVNPITDEVIVKENELITPEIARRIEELGLERIQVRSPMTCEAA
                     LGVCRSCYGMDLATGALVEDGMAVGIIAAQSIGEPGTQLTMRTFHIGGTVSTTVEESE
                     SKAKKEGIVKFTRMRFVANEEGK"
BASE COUNT          376 a          715 c          697 g          503 t
ORIGIN      
        1 ttcttcccct cctcgttagc cacgaaccgc atccgtgtga acttgacgat gccttccttc
       61 ttggctttcg attcgctctc ttcgaccgtc gtgctcaccg tgccgccgat atggaaagtc
      121 cgcatcgtaa gctgcgtgcc gggctcgccg atcgactgag cggcgatgat gcccaccgcc
      181 atgccatcct cgacgagcgc gcccgtcgcc aggtccatcc cgtagcacga gcggcagacg
      241 cccagggccg cctcacaggt catcgggctg cggacctgga tgcgttccag accgagttcc
      301 tcgatcctgc gggcgatttc cggcgtgatc agctcgtttt ccttcacgat cacttcgtcg
      361 gtgatcggat tgacgatgct ctggcggctg acgcggcccg tgatggcagc cgagagcttc
      421 acctcgacct tttcgccgcg gtaaatgact cccttggtga tgccctgcgt cgtgccgcag
      481 tcggtcgccg tgatcaccac attctgggcg acgtcggcca gcttgcgcgt gagatagccc
      541 gagtcggccg tcttgagggc cgtatcggcc agacctttgc gggcgccgtg cgtggagctg
      601 aagtactcca gcaccgtcag gccttcgcgg aaattcgact tgatcggcgt ttcgatgatt
      661 tcgccggtcg gcttggccat caaaccgcgc attccggcga gctgacgaat ttgctcgata
      721 ccaccgcggg cgccggaatc ggccatcaag tacacggggt tgacgtagcc gctgtcgccc
      781 cgaacgtccg tccgcatggc ttcgcgcatt tcgcccgtga tcgcttcacg ggcatgcgtc
      841 caggtatcca gcacctggtt gtagcgctcc ttctcggtaa tgacgccgcg ctcatagagc
      901 ttcttgaacc ggagcacctg cttttcggcc tccgaaatgt gcttgacctt ggtggacgga
      961 gtaagcaggt cgtccgtagc aaacgacagg ccgctgcggg ttgcctcccg gaagccgatc
     1021 tgcatcatgg cgtccaagag gtcgatcgtg gcccgccgcc cgtgcgactg gtagcaatcg
     1081 gaaatcacgc ccgccaaatc gccggacttc atccccttgt tgtagaaatc catgccgcgc
     1141 ggcaggatcg cattgaacag gatgcgccca taggtcgtct cgatgatcgt gcccggcttg
     1201 tgcttctcgt cgccttcgct tttgagcttc tggtgcgaag gcaggcggac cttgatccgc
     1261 gcgtgcagtt cgattttgcc catcgaatag gccagctccg cttcggccgg cgaggtaaac
     1321 gccatgcctt cgcccttgcg gccgggctgc tccagcgtga tgtagttgca acccatgacc
     1381 atgtcctgcg aggggctcat gatcggcttg ccgttcgccg gggcgaaaat gttgtgcggc
     1441 gagaggagca gcgtgtgcgc ctcgacctgg gcttcgatcg acagcggcag gtgcaccgcc
     1501 atctggtcgc cgtcgaagtc ggcgttaaag cccttgcaca ccagcgggtg gaggtgaatg
     1561 gcgttcccct cgaccaggat tggctcgaaa gccatgatgc ccatgcggtg cagcgtgggc
     1621 gcgcgattca gcagcacggg atggttctgg atcaccgctt ccaggatgtc ccagacctcc
     1681 tcgtccttcc gctccagcat cttcttggcc gacttgatcg tgtcggcatg ccccagctcc
     1741 ttgagcttgc gaatgatgaa cggctgatag agttccaggg cgatcttctt gggcaggccg
     1801 cactggtgca aacgcaggcg cggaccgacc acgatgaccg aacgagccga gtaatcgacg
     1861 cgcttgccga gcaggttctc gcgaaagcgg ccctgcttgc ccttgatcat gtcggtcagc
     1921 gacttgaggg ggcggttgct gctgccgagc accggccgtt tgcagcggtt gttgtcgaac
     1981 agggcatcga ccgactgctg gagcatccgc ttttcgttgc ggatgatcac ttccggcgcg
     2041 ttcagatcga ccagcttccg caaacggttg ttgcggttga tgatccggcg gtacaggtcg
     2101 ttcaaatcgc tggtggcgaa attgccgcta tcgagcagca ccagcgggcg caggtccggc
     2161 ggaatgaccg gaatcacgtc gagcaccatc cactcggggc ggttgtcgct atcgcggatc
     2221 gattcgacga tcttcagacg gttgatcaaa tcctttttct tttgcttgga attcgtctcc
     2281 gccaggtctt t
//
LOCUS       DASUGB010000349         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_594924, whole genome shotgun sequence.
ACCESSION   DASUGB010000349 DASUGB010000000
VERSION     DASUGB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594924"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="panD"
                     /locus_tag="VFV87_06715"
     CDS             complement(<1..331)
                     /gene="panD"
                     /locus_tag="VFV87_06715"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5103484.1"
                     /translation="MHRFLLKSKIHRATVTEANLDYEGSVTIDEELMRAANIIEFEQV
                     HIYDVTNGNRIITYAMVGPAGSGLICINGAAAHKVHPGDKVILATYSLLSEQEQQGFQ
                     PRIVQVNE"
     gene            complement(400..1251)
                     /gene="hisG"
                     /locus_tag="VFV87_06720"
     CDS             complement(400..1251)
                     /gene="hisG"
                     /locus_tag="VFV87_06720"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5103485.1"
                     /translation="MSNLRIGVPSKGRLSELAGELLKQAGLSFRRQERSLFARVGEMP
                     IDVTFLRTDDIPVLCAEGAIDMGLTGSDLLAESGADVLTRLELGMGRCKLALCVPEDS
                     SINTPSQLAGKRVATSFPNVTRKYLAEHKADVHLVNLGGSVEIMITLGVADAIVDLVE
                     TGSTLAANQLRMLAEIGRYETVLIQNKARRESELADRVVRRLEGVVIARSWSLLEYNL
                     PRNKLAEAEKITPGYSSPTINALEDPDWCAVKVMVRRGEVIEVMEKLEKLGAHAILET
                     QINNCRL"
     gene            complement(1280..1618)
                     /locus_tag="VFV87_06725"
     CDS             complement(1280..1618)
                     /locus_tag="VFV87_06725"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX5103486.1"
                     /translation="MSAVLHQLMEVLELRKVNPPPNSYTARLYAGGVNKIASKIREEA
                     DELIEAASEPGESGQRHVIYEASDVFYHLLVLLAHQGIKLDDVESEIARRFGQSGLDE
                     KASRPVAAAK"
     gene            1827..2876
                     /gene="miaA"
                     /locus_tag="VFV87_06730"
     CDS             1827..2876
                     /gene="miaA"
                     /locus_tag="VFV87_06730"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5103487.1"
                     /translation="MEDSLTKTLRSAPPALGCWYLTGPTASGKTKVGIELALRLNAEI
                     VSLDSMTVYQGMNIGTAKPSAEDRFRVPHHLLDLVPPSDDFSLSDYIDAAHAKIVEIR
                     GRGKEVLFVGGTPLYLKSLLRGVYQGPPADWEFRAAIERELQSLPLEALHERLQVIDP
                     LLAAKLHPNDKRRIIRGLEVFKLTGQRLSHLQTQFDEGRPAAQAKVFVLSWPRDQLHR
                     RIDARALQMFAAGLVEEVCGLLATYGALSRTALQAVGYREVIEYLRGQGTGDSGPGSG
                     VRSQESEHRGLSDSPSLLTRVKTRTHQFARRQETWFRSLSECTPVAMHELQVPAAVAE
                     RIIALRPTCPMLASS"
     gene            2924..3310
                     /locus_tag="VFV87_06735"
     CDS             2924..3310
                     /locus_tag="VFV87_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103488.1"
                     /translation="MRDSDSQPAEGMSAVSLILLFFGLAIVFIGLGLGLWLITIGYQA
                     LYHPEQVPLVGKVLTLLEKDDAFFEEVKTDTGVRYEGLGVRYGVLMLLLIVVLATLGS
                     AIRGFITAGSSMMQAAMGKPPPKQKT"
     gene            complement(3317..4060)
                     /locus_tag="VFV87_06740"
     CDS             complement(3317..4060)
                     /locus_tag="VFV87_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5103489.1"
                     /translation="MPRLLTLCAVVLLAAGCEPPGPVTIPAAGTLAKLGPGTHKEAVE
                     VPGVGNLKYTIEIPAGYDGSTPAPLVLALHYGYDGAVPEAYTGEGMIDSFRSGLAGLN
                     ALVIAPDVLGGDWTDARNEQAAVWLVNSAKATYSVDPKRVIITGYSMGGEGTWFIGSR
                     HQDVFTGAIPVAAPVAGSTDWKIPVYVIHSQADEIISHSAAKRHADAIKSKGGKLELK
                     SVTGLTHYDTGSYATHVGEGIQWLEGEWK"
     gene            complement(4138..4551)
                     /locus_tag="VFV87_06745"
     CDS             complement(4138..4551)
                     /locus_tag="VFV87_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5103490.1"
                     /translation="MSGELFVDTSGWFALLARRDPKHAAARRVLKRAKANRRGLVTTD
                     YVLDETATLLKARGEARLAESLFERVQSTPACTLHWTDPERFAAVRTFFLKHLDQAWS
                     FTDCLSFCVMKELKLREALTTDQHFEQAGFVALLK"
BASE COUNT          826 a         1511 c         1410 g          901 t
ORIGIN      
        1 gctcattcac ctgtacgatc cgcggctgaa agccttgctg ttcctgctct gacaggagcg
       61 aatacgtcgc tagaatcacc ttgtcgcccg gatgcacctt atgggcggcg gcgccgttga
      121 tgcaaatcaa gccgctgccg gcgggtccga ccatcgcgta ggtgatgatc cggttgccgt
      181 tggtcacgtc gtaaatgtgg acctgctcga actcgatgat gttcgccgcg cgcatcagct
      241 cctcgtcgat tgtcaccgag ccctcatagt cgaggttcgc ctcggtcacc gtcgcgcggt
      301 ggattttcga tttgaggaga aagcggtgca tgcaatcagt ccaaagtcca atgtccaaag
      361 tccaagatca tctcaacttt ggactttgga ccttggactc tacaaccggc agttattgat
      421 ttgcgtttca agaatcgcgt gcgccccgag cttctcgagt ttctccatca cctcgatcac
      481 ctcgccgcgg cgaaccatca ctttcacggc gcaccagtcg gggtcttcca atgcgttgat
      541 cgttgggctg ctgtagccgg gcgtgatctt ctcggcttcg gcgagcttgt tgcgcgggag
      601 gttgtattcg agtagcgacc agctccgggc gatgacgacc ccttccagcc gacggacgac
      661 gcggtcggcc agttcgcttt cgcggcgggc cttgttctgg atcagcaccg tttcgtagcg
      721 gccgatctcg gccagcatgc ggagttgatt cgcagccagc gtgctgccgg tctcgaccag
      781 atcgacgatc gcgtcggcca cgcccagcgt gatcatgatt tcgaccgagc cgccgagatt
      841 caccagatgc acgtccgctt tgtgctcggc gagatatttg cgcgtgacgt tgggaaagct
      901 cgtcgccacg cgcttgccag cgagttgcga gggcgtgttg atgctcgaat cttccggcac
      961 gcacagggcc agcttgcagc ggcccatacc cagctccagc cgcgttaaca catccgcgcc
     1021 cgactccgcc agcaaatcgc tgccggtcag ccccatgtcg atcgcccctt ccgcgcacag
     1081 gactggaatg tcgtcggtcc gcaggaaggt tacatcgatc ggcatctcgc cgacgcgggc
     1141 gaacaggctt cgctcttgcc ggcggaagct gagccccgct tgctttagca gttcgcccgc
     1201 caactcgctt agccggcctt tgctgggaac accgatacgc aggttggaca tcgtacaaac
     1261 tcagggagag gtgccgccgc tacttggccg cggcgaccgg ccgcgaggcc ttttcgtcca
     1321 gcccggactg gccgaagcgg cgggcgatct cggattcgac gtcgtccagc ttgattccct
     1381 ggtgggcgag cagcaccagc aggtgataaa acacgtcgga cgcttcgtag atcacgtgac
     1441 gctggcccga ttcgccgggt tcgctggccg cttcgattaa ttcgtcggct tcttcgcgga
     1501 tcttggaggc aatcttgttt acgccccccg cgtacaggcg ggccgtgtac gaattcggcg
     1561 gtggattgac tttgcgcagc tcgaggactt ccatcaactg gtgcaaaacg gcggacattg
     1621 gcggactcgc tggaaaaaag gctgaaaaag aggggtccag tcccaggttg tgccgcccgt
     1681 tgctttgcgg ggactagctt agaatcatcg taaatacgcc ccgttttccc tgcaatggct
     1741 aacatgaacc cgacgcgcaa gtgtgggcac accaacccgc agcgtaagcg agggagtgtg
     1801 tatcgagccg ccgctgacgg accgcaatgg aagattccct caccaagacc ctccgttccg
     1861 ccccgcccgc tcttggctgc tggtacctga ccggcccgac cgccagcggc aagacgaaag
     1921 tcggcatcga gctggccctg cgcctgaatg ccgagatcgt ctcgctggac tcgatgaccg
     1981 tgtatcaggg gatgaacatc ggcaccgcca agccctcggc cgaagaccgt ttccgcgtgc
     2041 cgcaccacct gctcgatctg gtccccccca gcgacgattt cagcctcagc gactacatcg
     2101 acgccgccca cgcgaagatc gttgagatcc gcggccgagg caaggaagtg ctcttcgtcg
     2161 gcgggacgcc gctgtatctc aagtcgcttc tccgcggcgt ctatcaaggt ccgccggctg
     2221 attgggagtt ccgcgcggcg atcgagcgtg aattgcagtc gctgccgctc gaagccttgc
     2281 acgaacgcct ccaggtcatc gacccgctgc tggcggccaa gctccatccg aacgacaaac
     2341 ggcggatcat ccgcggcctg gaggtattca aacttaccgg ccagcggctg agccacttgc
     2401 aaacgcagtt cgacgaaggc cgccccgcgg cgcaggccaa agtcttcgtc ctctcctggc
     2461 cccgcgacca gcttcaccgc cggatcgatg cccgcgccct gcaaatgttc gccgccggcc
     2521 tcgtcgagga agtttgcggg ctcctcgcca cgtacggcgc cctcagccga actgccctac
     2581 aagcggtggg ctaccgcgag gtgatcgaat acttgagggg acaggggaca ggggacagcg
     2641 gaccggggtc aggagtcagg agtcaggagt cagaacaccg cggactctcc gactctcctt
     2701 ctctcctcac gcgcgtcaaa acccgtacgc accaattcgc cagacgccag gaaacctggt
     2761 ttcgcagcct cagcgaatgt acgcccgtgg cgatgcacga actgcaggtc cccgctgcgg
     2821 tcgccgaacg gattatcgca ttgcggccaa cctgcccgat gctcgcgtcg tcgtgatacg
     2881 atgaatcggt tgtccgcact tcccatccct cgaatgactc gccatgcgcg actccgattc
     2941 ccagccggct gaaggaatgt cagccgtcag cctgatcctg ctgttcttcg gcctggcgat
     3001 cgtgttcatc ggcctgggtc tggggctgtg gctgatcacg attggctatc aggccctcta
     3061 tcacccggag caggtgccgc tggtgggcaa ggttctgacg ctcctggaaa aggacgacgc
     3121 cttcttcgag gaagtgaaga ccgacacagg ggtccgctac gagggcctcg gcgtccgcta
     3181 cggagtgctc atgctcctcc tgatcgtcgt cctcgccacc ctcggctccg ccatccgcgg
     3241 cttcatcacc gccggcagtt ccatgatgca ggcggcgatg ggcaaaccgc cgccgaagca
     3301 gaagacgtag caattcctac ttccactccc cttccagcca ctggatccct tcgccgacgt
     3361 gggtggcata ggagccggtg tcgtagtggg tcaggccggt gacgcttttc agttcgagct
     3421 tgccgccttt ggacttgatc gcatcggcgt gacgcttggc ggcgctgtgg gagatgatct
     3481 cgtcggcctg cgagtggatg acatagacgg ggatcttcca gtcggtgctt ccggcgacgg
     3541 gggccgcgac gggaatggcc ccggtgaaga catcctgatg gcgcgagccg atgaaccagg
     3601 ttccctcacc ccccatgctg tagccggtga tgatcacgcg cttgggatcg acggaatacg
     3661 tcgctttggc gctgttgacg agccagacgg ccgcttgttc gttgcgggcg tcggtccagt
     3721 cgccgccgag cacgtcggga gcgatgacga gcgcgttcaa acccgccagg ccggagcgga
     3781 acgagtcgat catcccctcc cccgtgtacg cctccggcac cgcgccgtcg tagccatagt
     3841 gcaaggccag cacgagcggc gctggcgtgc tgccgtcgta acccgccgga atctcgatcg
     3901 tgtacttcag gttccccacc cccggcacct cgacggcttc cttgtgcgtc cccggcccca
     3961 gcttcgccag cgtccccgcc gccggaatcg tcaccggccc cggcggctca caaccggccg
     4021 cgagaaggac gacggcacac agtgtcagca ggcggggcat agaaacctcc aaaagatcag
     4081 atgatggaga attatggagc atcggcggcc ggagatgcaa tcgcggggtt gctgccgtca
     4141 tttgagcagt gccacgaacc cggcctgctc aaagtgctga tccgtggtga gggcttcccg
     4201 cagcttgagc tctttcatca cgcagaagct caggcagtcg gtgaatgacc acgcttgatc
     4261 gaggtgcttc aagaagaacg ttcgcacggc cgcgaaccgc tcggggtcgg tccagtgcag
     4321 cgtgcaggcc ggcgtcgatt gcacgcgctc gaaaagcgat tccgccaggc gcgcttcacc
     4381 gcgggctttc agcagcgtcg ccgtttcgtc cagcacatag tcggtggtca ccagaccgcg
     4441 gcggttggct ttggcgcgct tcaggactcg ccgggctgcc gcgtgcttgg gatcgcgccg
     4501 cgccagtagg gcaaaccagc cgctcgtatc gacgaacaat tcaccgctca tagacgatcc
     4561 agtcgatgat ttcgttcgtc agcggctcca gcttgtcgct ggccaactcg tccagcgaat
     4621 agatcgaatc gtcgtcaccc gccgaccg
//
LOCUS       DASUGB010000350         6430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1569578, whole genome shotgun sequence.
ACCESSION   DASUGB010000350 DASUGB010000000
VERSION     DASUGB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569578"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFV87_06750"
     CDS             complement(<1..458)
                     /locus_tag="VFV87_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103491.1"
                     /translation="MFMQAGRGNSARLPFLLQRACPSALTAPWDDVVLAFTLNRALDK
                     VGLSESEKFASERVAAMWKVFWVGAFIAVALGALAIWASIQTTFLFVHPRAAGVRVVI
                     DKQTGRAVVASLAEESAARVKREQERRAKAPLSAPKVETAETNFDFGTMNP"
     gene            complement(493..1818)
                     /locus_tag="VFV87_06755"
     CDS             complement(493..1818)
                     /locus_tag="VFV87_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103492.1"
                     /translation="MKKRGFTLIELLVVVAIIGILVALLLPALALAREAARNAQCKSN
                     LRQFGIALQTHADKDPLQRYCTGASDYRRDGCMDTYGWVADIVNQGGGKVSEMLCPSS
                     PLVGSEKLNDLLTGNATTSLPKEGAPQARLDTGLCGPSGYKGFTSGGAGTLFAQTAAV
                     SSALPADDATDGAAAMGPGWPRIALISWGILEDGYNTNYCNSYYLVRTSPRVQNNASN
                     QPETASAPAAFKGLSGSLGPLTRRIAESGQVATSNIPLIGDSSPGDINEAVLCRTLVR
                     MDTDWVGQHLTGGIGGGEKTFIAAGSLLCEAFNDGPAQYDDAVPKIQLLGNQVNMNQQ
                     YLNDARGGVPSVGAALGNPDYLQDTRDFYALHGGNKGSANILMADGSVKTFYDLNSDK
                     YLNPGFPVPLTKTDIQYQGIGYRSPQVELSPGEVFSGVFLEKRVKAKFE"
     gene            complement(2028..3182)
                     /locus_tag="VFV87_06760"
     CDS             complement(2028..3182)
                     /locus_tag="VFV87_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103493.1"
                     /translation="MHTRRRQFACSSRSPQSAFTLVEMLTVIAIIGILIALLLPALGL
                     AREMARRAACMNNLKQFGQGMHIHAEASKEQFCSGAFDWLGDGAVTEMSWVGDLVQQG
                     TPVGKMLCPSNTARSSDVYNDLLNLDTTSPNFTNAAICVNVLGSPPSTDPGGAIVMNP
                     CRAIASAPLAPGDPARRQLVESQVYKQFYNTNYTATWFLVRSEISLDSSGNFRPAKPT
                     CVPLVGLSSRNSTSGPLRRPQVDTSITPASIVPVLVDGAQSGSVLADQVGDLAPGTPL
                     VVGFTRGPVVADMSGPPAFAPGTPKMTWWPAWQATRQDYRAFGVIHRGSSNVLFIDGS
                     VRSIKNLNKDDFLNNGFDPAIAIGSGYADNVIEMPEDEVYSLFSLNAKKL"
     gene            3618..5501
                     /gene="glmS"
                     /locus_tag="VFV87_06765"
     CDS             3618..5501
                     /gene="glmS"
                     /locus_tag="VFV87_06765"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5103494.1"
                     /translation="MCGIVGYVGSREAEQFLVEGLRRLEYRGYDSAGVATLSPDGRIV
                     TVKAVGRIESLAERLQENPAPGSIGIGHTRWATHGVPSEANAHPHFGGASSEPVVALA
                     HNGVIENYLAIKEKLIGEGYKFVSSTDTEAVAHLVASRLCKLEQRRDDFPADEPHAIL
                     LAAVSAAVRKLRGTYGLVILFRDYPNVIVAARQGSPLVIGVGQGEHFIASDASPLAGR
                     TDKIVYLADHQVALVTADKLEVTHAEHGRIDHAVHDLQIEENDVELGGFPHYMLKEIF
                     EQPEALENTMRGRLDHEDATAKFGGLNLSPQQIRGINRIILTACGTSWHAALVGEYLI
                     EELARIPVEVEYASELRYRNPPVDQGTLLFAITQSGETADTLAALREMKRKGHPTLAI
                     CNVVGSTIAQQADGGVYLHAGPEIGVASTKAYTSQVTTLALLALYFGRMRHLSYEAGE
                     RIIAQLHSLPDVIRRTLDVNNTVRQIAGKYCHATNVLYLGRQYNFPTALEGALKLKEI
                     SYIHAEGYPAAEMKHGPIALVDEHTPSVFVMPQGFVYDKVMSNLEEIKARGGPVIAIA
                     AEGDQNVARLADDVIYVPAVEEFLSPLVNIIPLQLLAYHIAILRGCDVDKPRNLAKSV
                     TVE"
     gene            complement(5635..6327)
                     /locus_tag="VFV87_06770"
     CDS             complement(5635..6327)
                     /locus_tag="VFV87_06770"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5103495.1"
                     /translation="MPLDVPKIVERLRFLREADRNLAVFGANGHEYKLRPRLDEAKVV
                     EFERRYSLRLPDDYRLFLIELGNGGAGPYYGIHGLDELYETQEDYWNDLSKPFPYRHQ
                     WHGPPELLKAINEADADESGDEDRRGELIDEYWRVASRDGSINICEYGCNLRFLLIVK
                     GPEFGRIWFDATPDLAGYSPVALSPAPPIERYGKWCITHNDTPEESRVGFAEWYHCWL
                     DWACRLVSGGAA"
BASE COUNT         1308 a         1903 c         2003 g         1216 t
ORIGIN      
        1 gggttcatcg tgccaaaatc aaaattcgtt tcggcggtct ctaccttcgg agctgaaagg
       61 ggggctttgg ctcggcgctc ttgctcccgt tttacgcggg cggccgactc ctcggcaagg
      121 gaagccacca cggcgcgtcc agtctgtttg tcgatcacaa ctcgcacgcc cgcggccctg
      181 ggatgcacga acaagaatgt cgtctgaatg ctggcccaaa ttgccagcgc acccagtgct
      241 actgcgatga acgctcccac ccagaatact ttccacatcg cagcaaccct ttcactggcg
      301 aatttctcgc tctcgcttag gcctaccttg tcgagcgccc gattcaatgt aaacgccaga
      361 acgacatcgt cccagggcgc cgtcaacgct gaggggcacg cgcgctgcaa caaaaacggc
      421 aggcgggcag agttgcctcg gcccgcctgc ataaacaatc acgattcagt ggctcttcgc
      481 gacaacgaca gactactcaa acttcgcctt gacgcgcttc tccaagaaga cgccgctgaa
      541 gacttctcca ggcgacaatt caacctgagg acttctgtac ccgatgcctt gatactggat
      601 gtcggtctta gtcaacggaa cggggaatcc cggattgagg tatttgtctg agttgaggtc
      661 gtagaacgtc ttcaccgaac cgtcggccat caggatgttg gccgagccct tattccctcc
      721 atgcaaggcg taaaagtcgc gcgtatcttg caggtaatca gggttgccga gggccgcgcc
      781 tacggaggga acgccacccc gcgcatcgtt cagatactgc tggttcatgt ttacctgatt
      841 gccgaggagt tggatcttcg gcacagcatc atcgtactgg gccggtccat cgttgaatgc
      901 ctcgcacagc aaggatcctg cggcaatgaa ggtcttctcc cctccaccga tacccccggt
      961 caggtgctgg ccgacccaat cagtatccat ccggaccaac gtgcgacaaa gcacggcttc
     1021 gttaatatcg ccgggcgaag aatcgccaat cagggggatg ttggaggtcg ctacctggcc
     1081 cgattcggcg atccgacgag tcaacggacc aagcgacccg ctcaggccct taaaagcggc
     1141 cggggcggaa gccgtttcgg gttggttgct ggcattgttc tggacgcgcg gcgaggtgcg
     1201 aacgaggtag taactgttgc agtaattcgt gttgtagcca tcttccagaa taccccacga
     1261 gatcagagca atccgcggcc agcctggtcc catggctgcc gcaccgtcgg tagcgtcgtc
     1321 ggccgggagg gcgctgctga cagcggccgt ttgcgcgaac aacgtgccgg cgccaccact
     1381 cgtaaatccc ttatatccgc tgggcccaca caacccggta tcgagccggg cctggggagc
     1441 cccttccttg ggaaggctcg tcgtggcatt gccggtcaac aggtcgttca acttctctga
     1501 gccaacaagc gggctgctgg ggcagagcat ttcgctcacc ttgcctcccc cctgattcac
     1561 gatgtcggcg acccaaccgt aggtgtccat gcacccgtcg cggcgataat cgctcgcgcc
     1621 ggtgcaatac cgctgcagcg gatccttgtc ggcatgggtc tgcagcgcga tgccgaactg
     1681 gcggagattg ctcttgcact gggcgttgcg ggccgcttcg cgggccagtg ccagcgctgg
     1741 caagagcagc gccacgagga tgccgatgat cgcgacgacc accagcaact cgatcaaagt
     1801 aaaaccacgt ttcttcatcg aaacactcct cacaggaacg ggatgggaaa aagtacgaac
     1861 cttgttcgaa tacgagccgt atcaatcgac aacgagaaca ccaagacgac gaaagtccgg
     1921 cgaggcgcac ctcctttgcc cgcacggggc caagcgtgtc tgctgtggat cgatcaaagt
     1981 tggcgacccg cacacgacaa gtgcgcggag ccgggaaaac tctgcggcta aagcttcttc
     2041 gcattgagcg aaaacaagct gtaaacttcg tcctcgggca tttcgatcac gttgtcggcg
     2101 tagccgcttc caatcgcaat cgcggggtca aagccattgt tgaggaaatc atccttgttc
     2161 aggtttttga tggagcggac gctgccgtcg atgaacagga cgttggagct tccgcggtga
     2221 attacgccga acgcacgata gtcttgccgt gtggcttgcc acgccggcca ccaggtcatt
     2281 ttgggcgtgc cgggcgcgaa tgccggtggc ccggacatgt ctgcgaccac cgggccacgc
     2341 gtgaacccga ccaccagcgg cgtgccgggg gccagatcgc ccacctggtc ggcgagcaca
     2401 ctccctgact gcgcgccatc gaccaggacc ggaacgatgc tggcgggcgt gatggacgta
     2461 tcaacctgcg gccgccgcaa cgggcccgag gtggaattgc ggctggacaa gccgacgagc
     2521 ggcacgcaag tcggcttggc ggggcggaag ttgccactgg aatcgagcga aatttcgctg
     2581 cggaccagga accatgttgc tgtgtagttc gtgttgtaga attgcttgta cacctgagat
     2641 tccaccaact gtcggcgggc ggggtcgcct ggagcaaggg gagcgctggc aatcgctcgg
     2701 caggggttca tgacaatcgc tccacccggg tccgtactcg gcggcgagcc caggacgttc
     2761 acgcaaatgg cggcgttggt gaaattgggt gatgtcgtat cgaggttgag caggtcgttg
     2821 tagacgtctg aggaccgggc agtgtttgag gggcacagca tcttgcccac gggcgtacct
     2881 tgctgaacca agtcgccgac ccagctcatc tcggtaaccg cgccgtctcc cagccaatcg
     2941 aatgcgccgg aacagaactg ctccttactc gcttcggcgt gtatgtgcat tccctgaccg
     3001 aactgcttga ggttgttcat gcacgcggcc cggcgggcca tctcccgcgc caggcccaag
     3061 gccggcagca gcagggcgat caggatgccg atgatggcga tcaccgtcag catttccacg
     3121 agggtgaacg cgctctgcgg cgatcgggaa ctgcacgcga attgtctgcg acgagtgtgc
     3181 atttgcgctg tgcgaaagtg gcccttcggg ttggtcgcgc gccagcggcg acttctccaa
     3241 gcagaatgcc ctcagtctgg ccagccaaat aaaggagacg tgatgcgccg gtgaacttcc
     3301 caagaggctt gcgttaagga acggagaagc cgtctggcgg tccagtacga atcgagtccg
     3361 attcgatcag gcaaccgatt ttgctgagcc actgtcatgg ccggccagct taggggcaaa
     3421 tatgagcggg cagcgctggc ggcgtgaatc ttcggagaat ctcatcgagg tcacgccgca
     3481 tgcgggaggg cggatccgca tcggacgatc cgccaccgcc tgctaaactg gcttttcccg
     3541 gccaggggcg acctagaatt tgggtggcgg gaactgcaga ttggtaggca ttgaacaggc
     3601 ggccaggaga cgcaagtatg tgtggcatcg tgggttacgt cggcagccgg gaggcggagc
     3661 agttcctggt cgagggtctg aggcggctgg agtaccgcgg atacgacagc gcgggagtgg
     3721 ccacgctttc gccggacggt cggatcgtca cggtcaaagc ggttgggcgg atcgaaagcc
     3781 tggccgagcg gctgcaagag aatcccgcgc cgggctcgat cggcatcggt cacacgcggt
     3841 gggccacgca cggcgttccg agcgaagcca atgcgcaccc gcacttcggc ggcgcgagca
     3901 gcgagcctgt ggtggcactc gctcacaacg gcgtgatcga gaattacctg gcgatcaaag
     3961 agaagctaat cggcgagggc tataagttcg tttcatcgac ggatacggag gcggtggccc
     4021 atctggtggc gagtcgcctg tgcaagcttg agcagcgacg cgacgacttt cccgccgatg
     4081 agccgcacgc gattctgctg gcggcggtga gtgcagccgt gcggaagctc cgcggcacgt
     4141 acggcctggt gattctgttc cgcgattatc cgaatgtcat cgtcgcggcc cggcagggaa
     4201 gcccgctggt gatcggggta gggcaggggg agcacttcat tgccagcgat gcttcgccgc
     4261 tggctggccg caccgacaaa atcgtctatc tggccgacca tcaggtcgcg ctcgttacgg
     4321 cagacaagct ggaggtaacg cacgcggagc acggtcgcat cgaccacgcc gttcacgatc
     4381 tgcagatcga ggagaacgac gtcgagctgg gcggctttcc gcattacatg ctcaaggaga
     4441 ttttcgagca gcccgaggcg ctggaaaaca caatgcgcgg ccgcttggat cacgaagacg
     4501 ccacggcgaa gttcggcggg ctgaatctct cgccccagca gattcgcgga attaaccgca
     4561 tcatcctgac ggcttgcggg acgagctggc atgcggctct ggtcggcgaa tacttgatcg
     4621 aagagctggc gcggattccg gtcgaggtcg aatatgccag cgaattgcgg tatcgcaatc
     4681 cgccggtcga tcaaggcacg ctgctgtttg cgattacgca gagcggcgag acggccgata
     4741 cgctggcggc gctgcgcgaa atgaagcgga aggggcatcc gacactggcg atttgcaatg
     4801 tcgtcggcag cacgatcgcc cagcaggcgg acggcggggt gtatctgcac gccgggccgg
     4861 aaatcggcgt ggcttccacg aaggcctaca cctcgcaggt gacgacgctg gcattgctcg
     4921 ccctttattt cggccgcatg cggcacctca gctacgaagc gggcgagcgg atcatcgccc
     4981 agcttcattc gctgccggac gtgatccggc ggacgctgga cgtgaacaac accgtgcggc
     5041 agatcgcggg caagtattgc cacgccacga acgtgctgta cctgggccgg cagtacaact
     5101 ttccgaccgc cctggaaggg gccctcaagc tgaaggagat cagctacatc cacgccgaag
     5161 gctacccggc tgcggagatg aaacacgggc cgatcgcact ggtcgatgag cacacgccga
     5221 gcgtgttcgt catgccgcag ggcttcgtct atgacaaagt gatgtcgaac ctggaggaga
     5281 tcaaggcccg cggcggcccg gtcatcgcca tcgccgccga gggggaccag aacgtggccc
     5341 ggctggcgga cgacgtgatc tatgtgccgg ccgtcgagga gttcctctcg ccgctggtga
     5401 acatcatccc gctgcagctt ttggcgtatc acatcgccat tctccgcggc tgcgacgtcg
     5461 ataagccgcg gaatttggcg aagagcgtga cggtggagta gggatctgta cggtggtcgc
     5521 ggccaccggc gatcctaacc gcgccgaatg acagcgttca ctaggcgggc cggctgtagg
     5581 gagcgtcgcg ttatggaaaa ccgtcatgcc gggcctgtca agttgagcgc tttgctatgc
     5641 cgcgcctcca ctgacaagcc ggcacgccca gtcgagccag caatgatacc actctgcgaa
     5701 gccaacccgg ctttcttctg gagtatcgtt gtgggtgatg caccacttac cgtaacgctc
     5761 aatgggcggc gccggactaa gcgccaccgg actgtaacca gccaagtcag gcgtggcgtc
     5821 aaaccagatg cgtccgaact ccgggccttt cacaatgagc aaaaaccgca agttgcatcc
     5881 gtattcacaa atgttgatcg acccatctcg agatgcgacg cgccaatact catcgattag
     5941 ttcgccacga cgatcttcgt cccccgattc atcagcatct gcttcattga tcgccttcag
     6001 cagttctggt gggccatgcc actgatggcg gtacgggaac ggctttgaca ggtcattcca
     6061 gtaatcctcc tgcgtctcgt acagttcatc caggccgtga ataccgtagt aggggcctgc
     6121 accgccgttg ccgagttcga tcaagaacag gcgataatcg tccggtagcc gaagcgagta
     6181 ccgacgttca aactccacga ccttcgcttc gtcgagacga gggcgaagct tgtactcgtg
     6241 cccattggct ccaaagacag cgaggttccg gtcggcttcg cgaagaaacc gaaggcgctc
     6301 tacaatcttg ggaacgtcta gcggcataac gattgattat cgcacaaatg ctcgcgatag
     6361 tttggaaacg cgcaaggtca atgtcaatca aatgttgtga tgggaattgg agttattgat
     6421 gcagcaccca
//
LOCUS       DASUGB010000351         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_318180, whole genome shotgun sequence.
ACCESSION   DASUGB010000351 DASUGB010000000
VERSION     DASUGB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318180"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFV87_06775"
     CDS             <1..493
                     /locus_tag="VFV87_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5103496.1"
                     /translation="RNLARAKLSHEKPGQTLQATALVHEAYLRLVDADRAQHWDSRGH
                     FFAAAAEAMRRILVENARRKQRVKHGGESERVELELAKLPTRMGAEELIALDEALERL
                     SKEDPLKARLVTLRYFGGLTIEQASESLNISRVTAFRYWTFARAWLHHEITGEQPIPS
                     ESD"
     gene            579..>2003
                     /locus_tag="VFV87_06780"
     CDS             579..>2003
                     /locus_tag="VFV87_06780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103497.1"
                     /translation="MQAEDIFLAALDKQTLIERQTFLETACGDNPGLRAQVEGLLKSH
                     EQAGSFLDAPLFDSPPTIDHLGLSDPRQATLLDEISLDFLAPSAEPGSIGRIGYYNIT
                     ETIGRGGMGVVLKARDTKLDRMVAIKVLAPELASNPTARKRFQREAHAAAAVVHQHIV
                     TIHAVDEDRLPYLVMECIVGQSLKEKIDREGHFKLIEILRIGQQVASGLAAAHAHGLI
                     HRDVKPANILLENGVERVRITDFGLARAVDDVGMTRTGEVAGTPQFMSPEQAQGLPMD
                     CRSDLFSLGSVLYAMCTGRPPFRAETAFATARRVCEDTPRPIREINPEIPDWLVAIID
                     RLLAKKPDERYQTAAEIAELLAQQLAQLQHPSLPAANAVPQSKIQNPKSKIGLRRWSL
                     AATAGIVLLLLAVSLTEATGVTHFAPTAIRIVRGEGTLVIELDDPTVQVSLGGEALSI
                     RGAGLQELRLLPGEYRLHATKDGHP"
BASE COUNT          403 a          638 c          605 g          357 t
ORIGIN      
        1 gcggaatctg gcacgggcca agctcagcca cgagaagccg gggcagacac tccaggcgac
       61 cgcgctggtc cacgaagcct atctgcggct ggtggatgcg gacagagccc aacattggga
      121 tagccgtgga cacttcttcg cggccgcggc cgaagcaatg cggcgaattc tggtggagaa
      181 cgcccggcgg aagcaacgtg ttaagcacgg cggagaaagc gaacgtgtgg agcttgaatt
      241 agccaaactg cccacgcgca tgggtgctga ggagttgatc gcgctcgatg aggcgctgga
      301 acgactttct aaggaagacc cgctcaaagc ccgcctcgtt acgctacgtt attttggtgg
      361 attgacgatc gagcaagcgt cggaatcgct gaatatttct cgagttacgg cgttccgtta
      421 ctggacattc gcgcgagcct ggctgcatca tgaaatcacc ggcgagcaac caataccgtc
      481 agaatctgac tgaaaatccg tcggctcatg gagcgcgctg accttgggcg tcgcattgat
      541 taatggagag gcatcgcccc gctgtgggag tgattttgat gcaggccgaa gatattttcc
      601 tcgcggcact cgacaagcag actttgatcg agcgtcagac gtttctggaa accgcctgcg
      661 gcgacaaccc gggcctgcgg gcccaggtgg aaggtctatt gaagtcccat gaacaagctg
      721 gcagttttct ggatgcgccg ctgttcgact ccccgcccac gatcgaccat ttggggcttt
      781 ccgatccgcg tcaggcgacg cttctcgacg agatctcgct cgactttctc gcgccctctg
      841 cggagcccgg gtcgatcgga cggatcggct attacaacat tacggaaacg atcggccgcg
      901 gcggcatggg cgtcgtgctg aaggcccgag ataccaagct ggaccgaatg gtcgctatca
      961 aggtgctggc cccggaactg gcttccaatc cgacggctcg caagcggttc cagcgcgagg
     1021 ctcacgccgc cgcagccgtc gttcaccagc acatcgtcac catccacgcg gtggatgaag
     1081 accgcctgcc gtacctcgtg atggaatgca tcgtcggcca gtcgctgaag gagaaaatcg
     1141 accgcgaagg acatttcaag ctgattgaaa tcctgcgaat cgggcagcag gtcgcctcgg
     1201 ggctggcggc cgcccacgcg cacggcctga tccaccgcga cgtgaagccg gccaacatcc
     1261 tgctggaaaa cggcgtcgaa cgggtgcgga tcactgattt cggcctggcc cgggccgtgg
     1321 atgacgttgg catgacccgg acaggtgaag tggccggcac gccgcaattc atgtcgcccg
     1381 agcaggctca gggcctgccg atggattgcc gcagcgacct gttcagctta ggcagcgtgc
     1441 tctatgccat gtgcaccggc cggccgccat tccgggccga gaccgccttt gccaccgccc
     1501 gccgcgtctg cgaggacacg ccccgcccga ttcgcgaaat caatccggaa attccggact
     1561 ggctggtcgc gatcatcgat cgcctgctgg cgaaaaagcc cgacgagcgc tatcaaaccg
     1621 ccgctgagat cgccgaattg ctggcccagc agcttgccca gctacaacac ccctccctgc
     1681 cggccgcgaa tgctgttccg caatccaaaa tccaaaatcc aaaatccaaa atcggcctcc
     1741 gccgctggtc cctcgccgcc actgccggca ttgtcctcct gctactcgcc gtttcgctga
     1801 cagaagcgac gggtgtgacc cacttcgcgc cgaccgccat tcgcatcgtc cgcggcgaag
     1861 gcacgctggt gatcgagctc gatgatccga cggtgcaagt ctccctcggt ggtgaagcat
     1921 tgagcatccg cggcgccggc ctccaagaac tcaggctgct cccgggagaa taccggctgc
     1981 acgccaccaa ggacggccac ccg
//
LOCUS       DASUGB010000352         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1846183, whole genome shotgun sequence.
ACCESSION   DASUGB010000352 DASUGB010000000
VERSION     DASUGB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846183"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /gene="secF"
                     /locus_tag="VFV87_06785"
     CDS             <1..2184
                     /gene="secF"
                     /locus_tag="VFV87_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5103498.1"
                     /translation="MAKGTALRMAIRNGFDRAFVTIVDSNLTTLLTAIVLYAIGTDQV
                     RGFGITLILGILTSMFTAIFATRTILEFGERARFIRKLSMTHFLTNPQIDWCSYLKPA
                     LILSSLLIAAGLVATVARGVGIFDIDLAGGSSVVVALKEPLAEDVVRKKLAQEFEKEV
                     DEVTKGRVEFYVHGMEMTGEKPGTVYKVDSSLAEDTKLQRVVREAFLTPDGKDGLRTY
                     QVSIGSLTEEPVEAPGTPPTIGAPPGQEPADTTQPADKTAEQKSAPAPEPTPKTDKAS
                     QPAEPPATQKPAAETPADENSVEKPAEEKSDSKSADKTSEDADEPSECQEKETSEETK
                     AEAVPAETPAADGKPADAQKAAEPAEQPAAGESQPAEKQPAKEKPAKATPAGTTPAEP
                     PAAETTPAQAPSVESPSGTTPAVPAAPKPQVQTTAELNFVNSPISADAVRERIRNSSV
                     AVLNEEVEADLVTPEDKNWNGRDNSAFERWTVTLPLSKSQAQAVLDRVKSSLAEEVVW
                     QTSSKIGGQVSSDTRWRAVGAIFVSLLGILGYLWFRFQKAVWGIAAIVALAHDALIML
                     GGIAVSYWLAGPLGFMQVEEFKISLPVVAAFLTLIGFSVNDTIVIFDRIREVRGKSPD
                     VTAKMINDSVNQTLSRTILTSGTVLITVVILFFFGGPGIHAFAFAMICGVLSGTYSTV
                     FIAAPLVLWLLGKPASDPAARQRSREARAAGDKGGGAVTRPSRTA"
     gene            2427..3005
                     /locus_tag="VFV87_06790"
     CDS             2427..3005
                     /locus_tag="VFV87_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103499.1"
                     /translation="MRAKQLACAAGVLGAGVVLAWPFRQDAPPALPPPAAPIDLALRR
                     PDVALAAGPPGESSPAVGLDVIDGAGNLVHVQTAAYSSTRPSLEDLGPPPEMPIDFAP
                     VAVSPPSTAYVPRSLAPPSLAQKPMRPRQYTLRDGDTLEDLAKRMLGSESRAREIFEA
                     NRAVLPSPDLLPIGVTIVIPPRVQQADELTSE"
     gene            complement(3041..3991)
                     /locus_tag="VFV87_06795"
     CDS             complement(3041..3991)
                     /locus_tag="VFV87_06795"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5103500.1"
                     /translation="MSFRRVLAGVFALLVATVLLSVPARPVLAQAAKGAKKAELLQPE
                     DLTLETKDGVALRCTYYPGAAKKASVPVIMVHGWGGTRSEYDSLALGLQGLGHTVIVP
                     DLRGHGQSTVVKLPNGDNETIDPSKLRAKDLEAMVLDLDACKKFLLDKNNAGECNIEM
                     LCVVGAEFGSIVAMQYAVYDWAKPRLPAYKLGQDVKALVLLTPMQSFKGLTTRVPMSS
                     DIIRSKLAMLLVAGREDSKGTAEAKRMHNSFESSRPKVDPEDQRTKLDLFLVQPDTSL
                     TGTKLLGSGLNIPTNIANFIKFRLADKQEELAWMNRENPL"
     gene            complement(4173..>4631)
                     /locus_tag="VFV87_06800"
     CDS             complement(4173..>4631)
                     /locus_tag="VFV87_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5103501.1"
                     /translation="GLPDALDLMVVCVEAGLGLDQAMRKVAEEMKKSYRVIAEEFSIC
                     NLQLQMGRARQHVLQELGTRSGVDDLKSLSSILIQADKFGSSIAQALRVQSESMRTRR
                     KQIAEEKAAKTAVKLIFPLVLFIFPGIFVVLVGPAAITMVREMFPAMQGG"
BASE COUNT          869 a         1490 c         1456 g          816 t
ORIGIN      
        1 atggccaaag gaaccgccct gcgaatggcg atccgcaacg gctttgaccg ggcgtttgtg
       61 acgatcgtgg acagcaactt gacgacgctg ctcacggcca tcgtgctcta tgccatcggc
      121 accgatcagg tccgcggctt cggcatcacg ctgatcctgg gcatcctgac gagcatgttc
      181 acggcaatct tcgccacccg cacgatcctg gaattcggcg agcgggcccg ctttatccgc
      241 aagctctcga tgacccattt cctcaccaat ccgcaaatcg attggtgcag ctatctgaag
      301 ccggcgctga tcctgtcttc gttgttgatc gccgccggcc tggtggcgac ggtggcccgc
      361 ggtgtcggca tcttcgatat cgatctggcc ggcggcagct cagtcgtggt ggcgctcaaa
      421 gagccgctgg ctgaggacgt ggttcgcaag aaactcgccc aggagttcga aaaggaagtt
      481 gatgaagtta cgaagggacg cgtggagttc tacgtccacg gcatggagat gaccggtgaa
      541 aagcccggaa ccgtctataa ggtcgatagc tcgctggcgg aagatacaaa gctgcagcgc
      601 gtcgtgcgcg aggcgtttct cacgccggac ggcaaggatg ggctgcggac ctaccaggtt
      661 tccatcggca gcttgaccga agagccggtg gaagctcctg gcacgccgcc caccattggc
      721 gccccgccag gccaagagcc ggcggacaca acccaaccgg cagacaaaac agcggagcag
      781 aaatcggcac cagccccaga acccacaccc aagaccgaca aagcctctca gccggccgaa
      841 ccacccgcga cgcagaagcc ggctgcagaa acgccggcgg acgaaaattc cgtggagaaa
      901 ccggctgaag aaaagtcaga ttctaaatcc gccgacaaaa cttccgagga tgccgacgag
      961 ccttcggaat gccaggaaaa agagacgtcc gaggaaacga aagccgaagc cgttcctgca
     1021 gagacgccag ccgcagatgg caaacctgca gacgcccaga aagcggccga accggcagag
     1081 caacccgcag cgggcgaatc ccagccggca gaaaagcagc ctgcgaagga gaaacccgcg
     1141 aaagcaaccc ctgcggggac cactcccgcg gagccgccgg cggctgaaac gacgcctgcc
     1201 caggctccat ccgtcgagtc gccgtctgga actacgcccg ccgtcccggc tgctcccaag
     1261 ccgcaagtgc aaacgaccgc ggagctgaat ttcgtcaata gcccgatcag tgcggacgcc
     1321 gtgcgcgagc ggatccgcaa cagctccgtc gcggtcctta acgaagaagt cgaagccgac
     1381 cttgttacgc ccgaagacaa gaactggaac ggcagagaca attcggcctt tgaacggtgg
     1441 acggtcacgc tgcccctgag caagtcgcaa gcgcaggcgg tactggaccg cgtcaagtcc
     1501 tctctcgccg aagaggtcgt ctggcagacg tcgagcaaaa tcggcggcca ggtctcgagc
     1561 gatacccgct ggcgcgccgt cggggcaatt ttcgtcagcc tgctcggcat cctgggttat
     1621 ctctggttcc gcttccagaa agcggtttgg ggcatcgccg ccatcgtggc cctggcccat
     1681 gacgcgctga tcatgctcgg cgggatcgcc gtgagttatt ggctcgccgg gccgctgggg
     1741 ttcatgcagg tcgaggagtt caagatcagc ctgccggtcg tcgccgcgtt cctgacgctg
     1801 atcggcttct cggtcaacga cacgatcgtg attttcgacc gcatccgcga agtccgtggc
     1861 aagagcccgg acgtgacggc caaaatgatc aacgacagcg tcaaccagac cctcagccgc
     1921 acgatcctca cgtcgggcac ggtgctgatt acggtggtga tcctgttctt tttcggcggg
     1981 ccgggcatcc atgccttcgc cttcgccatg atctgcggcg tgctttcggg cacgtacagc
     2041 acggtgttca tcgccgcgcc gctggtgctc tggctgctcg gcaagccggc ctccgacccc
     2101 gccgcccggc agcgaagtcg cgaagcccgc gccgcgggcg acaagggcgg cggcgcggtc
     2161 accaggccct cgcgaacggc gtaacttacc agttcggcgg tgtggacttt gccgcctgcc
     2221 ggggagtttg acggcgggcg gttttctttt ccagcggccg gcgattcaag tcgatgacgg
     2281 atggtgagag cgtgcgctcg cgggccgtcc aggcggctct gaggcacgct ctgcgtggct
     2341 gccgcgggaa ttgcgggcag ccggatcaga atccaagcgc tgtgatggca ggatgacgct
     2401 cggcgcggag cggagactgc cttccgatgc gcgccaaaca gcttgcctgc gccgccggcg
     2461 tgctcggcgc cggcgtggtc ctggcgtggc cgtttcgcca ggatgccccc cctgcccttc
     2521 cgccgcctgc ggcgccgatc gacctggcgc tgcgccggcc ggatgttgcg ctggccgccg
     2581 gcccgcccgg cgaaagttcg cccgcggtcg gcctggacgt gatcgacggc gcggggaatc
     2641 tcgtccacgt gcagacagcg gcttattcga gcacgcggcc gagcctcgag gacctgggtc
     2701 cgccgccgga aatgccgatc gactttgccc ccgttgccgt gtcgccgcct tccaccgcct
     2761 atgtgcctcg ctcgctcgcg ccgccgtccc tcgcccagaa gccaatgcgc ccgcggcaat
     2821 acacgctccg cgacggcgat acgctggaag acctggctaa gcggatgctc ggcagcgaat
     2881 cacgggcgag ggagattttc gaggcgaatc gcgccgtgtt gccctcgccg gacttgttgc
     2941 cgatcggcgt aacgattgtc attccgccac gtgtgcagca ggcggatgag ctaacgagcg
     3001 aatagtgggt tccaatctgg cgtgacgccc gctgcaccgt tcagagcggg ttctcgcggt
     3061 tcatccaggc cagttcttcc tgcttatccg ccagccggaa tttgatgaag ttcgcgatgt
     3121 tggtcggaat attcagcccg ctgcccagga gcttcgtgcc ggtcaggctg gtatcgggct
     3181 gcacgaggaa cagatcgagc ttggtgcgct ggtcctcggg atctactttg ggccggctcg
     3241 actcaaagga attgtgcatc cgcttggctt ccgcggttcc tttggaatcc tcgcgcccgg
     3301 ccacgagcaa catcgcgagc ttggaacgga taatgtcgct cgacatcggc acgcgggttg
     3361 tcaagccttt gaacgactgc atcggcgtga gcagcaccag ggccttcacg tcctgcccca
     3421 gcttgtaggc tggcagacga ggcttggccc agtcatagac ggcgtattgc atagccacga
     3481 tcgatccgaa ttcggccccc acgacgcaca acatttcaat gttgcattcg ccggcgttgt
     3541 tcttatcgag caggaatttc ttgcaggcat cgaggtcgag gaccatcgcc tccaggtcct
     3601 tggctcgaag cttggaagga tcgatcgttt cgttgtcgcc gttggggagc ttgaccaccg
     3661 tgctttggcc gtgtccgcgg agatcgggca cgatcaccgt gtgccccagg ccctgcaggc
     3721 cgagggccag cgaatcgtac tcgctccgag tacctcccca tccgtggacc atgatcaccg
     3781 ggaccgaagc ctttttcgcg gcgcccggat agtaggtgca gcgcagtgcg acaccgtcct
     3841 tcgtttcgag cgtcaagtcc tcgggttgga ggagctcggc ctttttcgcg cccttggcag
     3901 cctgggccag gaccggccgg gcgggcacgg aaagcaggac ggtggcaacg agcaacgcga
     3961 aaacgcctgc gagaactcga cgaaaggaca ttactcacct accggcaatg agaacggtcg
     4021 aactcccctg gctgccgacc acaagagttt atgttaagcc gcttggcgcg acaggtcaac
     4081 gaagtcgccc aagcgaagcc tggcgagtgt ggccgagctt gcgccgctga tcgtggaagc
     4141 gatggctcca gacgcctctc gggcgtcagc cattatccgc cctgcatggc cgggaacatc
     4201 tcgcggacca tcgtgatggc ggcagggccg accagcacca cgaagatgcc ggggaagatg
     4261 aacaacacca gcgggaagat cagcttgacc gccgtcttgg ccgccttttc ttccgcaatc
     4321 tgcttgcggc gggtccgcat cgattcgctt tgcacgcgca gcgcctgagc gatcgacgag
     4381 ccgaacttgt cggcctggat cagaatcgag gacagcgact taagatcgtc cacgccgctg
     4441 cgggtgccca attcctgcag cacgtgctgg cgggcgcggc ccatctggag ctgcaggttg
     4501 cagatgctaa attcctcggc aatcacccgg tacgacttct tcatttcctc ggcgactttc
     4561 cgcatcgcct ggtccaggcc caaacccgct tcgacgcaga cgaccatcag gtctagcgcg
     4621 tccggcaggc c
//
LOCUS       DASUGB010000353         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1846234, whole genome shotgun sequence.
ACCESSION   DASUGB010000353 DASUGB010000000
VERSION     DASUGB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846234"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFV87_06805"
     CDS             <1..494
                     /locus_tag="VFV87_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103502.1"
                     /translation="EDRPELFPRMAIKSLTAEQLYDCLIEAMRRREVSMQVQQLGFNR
                     GFDQNRLAFLARFRAPTQGATEYEGGIPQALTLMNGANIRQATDLGQSDLLVALEAPF
                     FSNERRVEVLFYSTLSRSPSDEERTKFVAYVEQGGTNNSSKQALGDVLWALLNSAEFV
                     LNH"
     gene            575..1906
                     /locus_tag="VFV87_06810"
     CDS             575..1906
                     /locus_tag="VFV87_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103503.1"
                     /translation="MTQFHRRDWLRAAELSLAGFSFSGWMPALANAAAAADGSRRRHC
                     VLLWMPGGPSQMDTFDLKPEHKNGGPFKEIETSVPGLKISEHLPKLAAQAENLAIIRS
                     LSTAEGDHSRGTYLMHTGHRPGGPITFPTMGSTISKALGSDDAELPNFISIAPFTALN
                     NAAFGPGFLGPRHGPLTVGATNVFQQPQQQPAGGYARLGVDDLHSAGVSREQEDARLD
                     LWSTLQTGFLKTHPTASPKAQHTVYERAVRMMRSEAAQAFELDEEPDKVRDSYGRGEF
                     GQGCLMARRLIERGVPFIEIALRGTGGGLGWDTHQQNFQRVQQLSAELDAGWATLMSE
                     LKDRGLLESTTIVWLGEFGRTPTINPGGGRDHYPKAWTTVLAGGGIQGGQAYGRTSAG
                     GDTVEEGQVDVPDVLATLCQAVGVDPRTQNISDLGRPIRIAEGQPIEAVLA"
     gene            1976..>2794
                     /locus_tag="VFV87_06815"
     CDS             1976..>2794
                     /locus_tag="VFV87_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103504.1"
                     /translation="MMHRIAAILAILSLATAVDRAAAQAPATKPAAPSQTEIRKQLID
                     ATKELAESLKALATAEAAAVDAKVEAEKARSQTESLRAKVAKLNRQLLDAAQATQAIV
                     ARGGPRGAAGLERENADPQDPYERFAILTPGGPVVVQVALTIDGQPFRTVREKFIDEM
                     LAAADRDKDGKATWDEALDSPRFTLGRLRLTSDQQKQPFVRGLDRNADGLVDRPEVRL
                     FVAQTFGAPTFSLGGYSTAYGGFAGNVVVVNGQVIATGGGQADVRALLDLDADGV"
BASE COUNT          517 a          895 c          956 g          426 t
ORIGIN      
        1 cggaagatcg gcccgagctc tttccccgga tggcgatcaa gagcctcacc gccgagcagc
       61 tctacgattg cctgatcgag gcgatgcggc ggcgcgaggt gagcatgcag gtgcagcaac
      121 tggggttcaa ccgcggcttc gatcagaatc ggctggcgtt cctggcccgt tttcgagcgc
      181 cgacgcaggg agcgaccgaa tacgaagggg gcatcccgca ggcgctgacg ctgatgaacg
      241 gggcgaatat tcgccaggcg accgatttgg gccagagcga cctgctcgtt gccctggaag
      301 cgccgttctt cagcaacgag cgccgcgtgg aggtgctgtt ctattccaca ctctcgcgct
      361 cgccgagcga cgaggagcgg accaagttcg tcgcctatgt cgagcaaggc ggaaccaaca
      421 acagcagcaa gcaggccctg ggcgatgtgc tgtgggccct gctcaatagt gccgagtttg
      481 tgctcaatca ttgaagtagc cgcgttcgcc agaacgcggg caaagcggac gggcgtcccg
      541 cactctggcg agtgcggcta caggagtgga cgagatgacg caatttcatc gtcgcgattg
      601 gttgcgggct gcggagctca gtctggctgg gtttagcttt agcgggtgga tgccggcgct
      661 ggcgaatgcc gcggcggcgg cggatggcag tcgtcgccgg cattgcgtcc tcctgtggat
      721 gccgggcggg ccgagccaga tggacacgtt cgacctcaag ccggagcaca agaacggcgg
      781 cccgttcaag gaaatcgaga cgagcgtgcc gggcctgaag atcagcgagc acctgcccaa
      841 gctggcggca caggcggaga acctggcgat catccgcagc ctgagcacgg ccgaagggga
      901 ccacagccgc ggcacgtacc tgatgcacac ggggcaccga ccgggcgggc cgatcacgtt
      961 cccgacgatg ggctcgacga tctcgaaggc cctgggaagc gatgacgcgg agctgccgaa
     1021 ctttatcagc attgccccgt tcacggcgct caacaatgcg gcgttcgggc cgggcttcct
     1081 tggcccgcgc catggcccgc tgacggtcgg cgcgaccaac gtttttcagc agccgcagca
     1141 gcagccggcc ggcggctatg ccaggctggg ggtcgatgac ctgcacagcg ccggcgtcag
     1201 ccgcgagcag gaagacgccc ggctggatct gtggagcacg ctgcaaaccg gttttttgaa
     1261 gacgcatccc acagcgtccc ccaaggccca gcacacggtc tatgagcggg ccgtgcggat
     1321 gatgcggagc gaagcggccc aggcatttga gctggacgaa gagccggaca aagttcgcga
     1381 ttcctacggc cgcggcgagt ttggccaggg ctgcctgatg gcccgccgcc tgatcgagcg
     1441 gggcgtgccg ttcatcgaaa ttgcgctccg cggcaccggc ggagggctcg gctgggatac
     1501 gcaccagcag aacttccagc gcgtccagca gctttccgcc gagctcgatg ccggctgggc
     1561 gacgctgatg agcgaactga aagatcgcgg gctgctggaa tcgacgacga tcgtctggct
     1621 cggcgaattc ggccgcacgc ccacgatcaa tccaggcggc ggtcgcgatc attacccgaa
     1681 ggcctggacg acggtcctcg ccggcggcgg catccagggc ggccaggctt acggccgcac
     1741 cagcgccggc ggcgacacgg tcgaagaagg ccaggtcgat gtacccgacg tgctcgccac
     1801 gctttgccag gccgtcggcg tcgatccgcg cacgcaaaac atttcggatc tgggccgccc
     1861 gatccgcatt gccgaaggcc agccgatcga ggcagtcctg gcctaatcac accaacccga
     1921 cgcgcaagcg agggagtcca aaccaacccg aagcgtcagc gagggagtcc atccaatgat
     1981 gcatcgcata gctgccattc tcgcgatcct gtctctcgcc accgcagtgg atcgggcggc
     2041 cgcacaggcc cctgcaacca aaccggccgc cccatcgcag actgaaatcc gcaagcagct
     2101 catcgacgcc accaaagagc tggccgagtc gctcaaggct ctggccacgg cggaagcggc
     2161 cgctgtggat gccaaagtgg aggcggagaa agcgcggagc cagaccgaat cgctgcgagc
     2221 caaagtggcc aagctcaacc ggcagttgct cgacgcggcc caggccaccc aggcgatcgt
     2281 cgcccgcggc ggcccgcgcg gcgcagcggg cttggagcgc gagaatgccg atccacagga
     2341 cccttacgag cgtttcgcga ttctgacgcc cggcggcccg gttgtcgtgc aagtcgctct
     2401 gacgattgac ggccagccct tccgcactgt ccgcgagaag ttcatcgacg agatgctcgc
     2461 cgccgccgac agagacaagg acggcaaggc aacgtgggac gaagccctgg actccccgcg
     2521 cttcacgctc ggccgattgc ggctcacttc cgaccagcag aagcagcctt tcgtgcgggg
     2581 gctcgacagg aatgccgacg ggctcgtcga tcggccggag gtgcggctgt ttgtggctca
     2641 gacgttcggc gcgccgacgt tctctctggg cggctatagc acggcgtatg gcgggtttgc
     2701 cggcaatgtc gtggtggtca acgggcaggt catcgcgacc ggcggcggcc aggccgacgt
     2761 gcgggcactg ttggatctgg atgccgacgg cgtt
//
LOCUS       DASUGB010000354         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_40243,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000354 DASUGB010000000
VERSION     DASUGB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40243"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFV87_06820"
     CDS             complement(<1..708)
                     /locus_tag="VFV87_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103505.1"
                     /translation="MRRRRIRRRGLAPLELVLAIPLLLFVLALSVIFGTVACWRVRGQ
                     SVARDAIWSHRWPRTEPWGVDHDPRPLEWPSPAVINNNAGPPLGSLDHGAFQHPVIRG
                     PLPGNIVVNDRLFDPTLGLRRGHAEIVRTPPMLGTLGDYRLDLEHQLFDGKWQFRQMG
                     VGSNTNRRIPVIYPNLLDLPQTGGLKAQYQQAIQAIVSSPLKPDLAVLDRDAEIYAWY
                     GYYPDFHPRFQRFCSLDF"
     gene            complement(801..2114)
                     /locus_tag="VFV87_06825"
     CDS             complement(801..2114)
                     /locus_tag="VFV87_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX5103506.1"
                     /translation="MDLHKDERGSISFATVFAMLLLTMLLGMVINTERQIDSKVKLQN
                     AADASTYSGGVVLARGMNSLAFSNHMLCEVMALTAFFREGRDRHAEPLVPEILAAWDR
                     IGPVLGNSGFEKFERLGDAIPQKTPLEQQMVTAYGEWIYASSEIVLPVLETILNEEMI
                     PQYQRQVVQATPLVSQTAAAEIADRHTGNPSARDQARGRVIGVFWRTLVDPVGGGSEG
                     ARGALPAVDPTVELNYQSQARADRDRYARHYLNLWNNVMMRPFDQEAKMSQFGSLWRS
                     FTCGQLDQLLDEHSGGNFPHMIRTAPGASGSVNNDLEFDYMFVGVAYRAKVVPAAPNL
                     FVDSLAADNQAFAQGLLFAPRPRVVSYWTDQYGIRHGIRNNSPRHWDLWNQNWSFQLV
                     PATSISIGPILTTQPQTPFSAGVSGLTLPTLQNVDPADIRRVTTH"
     gene            complement(2203..3144)
                     /locus_tag="VFV87_06830"
     CDS             complement(2203..3144)
                     /locus_tag="VFV87_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103507.1"
                     /translation="MAHVKGEAMLRGVLLDLWPFALALAATAAALAVLARSLRPEWRR
                     QILARLHRDEVGGVQSVSFVLAAPVFVMLMLLAVQITQLMIGLMIVHYAAYAAARSAT
                     VWIPARMEPIEQVGENRIGNRVLIGSYAYGSEYLIEPGTPKYEQIRTAAALACLPIAP
                     SRDIGIAGGDPMTGSIQALYASYAPAAMSNGRIPQRLANKWAYANAATEIEIHTFHRN
                     DEPPLIEYPPYHRDSEFKPGEIGWRDQIRVTVTHKFALLPGPGRLLARRANESRYPDR
                     ISPQVLQQAGVYYVPLSATATMVGEGEKSVRPYVYVD"
     gene            complement(3132..>3546)
                     /locus_tag="VFV87_06835"
     CDS             complement(3132..>3546)
                     /locus_tag="VFV87_06835"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5103508.1"
                     /translation="ARDWQPLVGWLPVSEAVGGFLVCGLVVVCCFVVFPIGGGDVKLL
                     AMIGALAGLNKGLEVLLWTFIFGGCVGLVILIWRLGPLALLLRGWQLLVGVLTLGIWL
                     RPPEKEQETLKLPVFLGPCAAAALIATFVPWQWPM"
BASE COUNT          681 a         1131 c         1148 g          586 t
ORIGIN      
        1 gaaatcgagg ctgcaaaatc gctggaaccg cggatgaaaa tcgggatagt agccgtacca
       61 ggcgtagatt tcggcatcgc ggtccaaaac cgccagatcg ggcttcagcg gcgagctgac
      121 gatcgcctga atcgcctgct ggtattgagc cttcaagccg cccgtctgcg gcaggtcgag
      181 cagattcgga tagatcaccg gaatccgccg gttggtgttg ctccccactc ccatctggcg
      241 gaactgccac ttcccgtcga agagctggtg ctccaggtcc agccgatagt cgcccagcgt
      301 gccgagcatc ggcggcgtgc gaacgatctc ggcgtgtccg cggcggaggc cgagcgtggg
      361 atcgaacagg cggtcgttga cgacgatgtt gcccggcagc gggccgcgaa tcacgggatg
      421 ctggaaggcg ccgtgatcga gcgagcccag cggcgggccg gcattgttgt tgataaccgc
      481 gggcgacggc cattccagtg gacgaggatc gtgatcgacg ccccagggct cggtgcgcgg
      541 ccagcggtgg ctccagatcg catcgcgggc caccgactgg ccgcgcacgc gccagcaggc
      601 gaccgtgccg aagatcaccg agagggccag cacaaacagc aacagcggga tcgccagcac
      661 cagctccaac ggcgccaatc cgcggcggcg gatgcgacga cgtcgcatcg taacccgaag
      721 cgtaagcgag ggagcgcacg aggacgctac aacgcgattc cctcgcttac gcttcgggtt
      781 actgttctgg gagtccatca tcagtgggtc gtcacccggc gaatgtcggc cggatcgacg
      841 ttctgcaggg tcggcagcgt caatccgctg acgcccgcag aaaatggcgt ctgcggctgc
      901 gtggtaagga tcggtccgat gctgatcgag gttgccggca cgagctggaa cgaccagttc
      961 tgattccaca ggtcccagtg ccggggcgaa ttgttccgaa tcccgtgccg aatgccatac
     1021 tggtcggtcc aatacgagac gacccgcggc cggggagcaa agagcaatcc ctgagcgaac
     1081 gcctggttat ctgctgccag cgaatcgacg aacagattcg gcgccgccgg tacgaccttc
     1141 gcgcgatagg ccacgcccac gaacatgtaa tcgaattcca ggtcgttgtt gacgctgccg
     1201 ctggcgccgg gcgctgtgcg gatcatgtgc gggaaattgc cgccactgtg ctcgtcgaga
     1261 agctgatcga gctggccgca ggtgaagctc cgccacaggc tgccgaattg tgacatcttc
     1321 gcttcctgat cgaaggggcg catcatcacg ttgttccaca gattcaggta gtggcgggca
     1381 tagcggtcgc ggtcggcccg ggcctggctc tggtagttga gctcgaccgt cggatcgacg
     1441 gcgggcaagg cgccgcgggc gccctcgctg ccgccgccga ccggatcgac aagcgtccgc
     1501 cagaagacgc cgatcacccg cccgcgggcc tggtcgcggg ccgaaggatt ccccgtgtgg
     1561 cggtcggcaa tttccgcggc tgcggtctgg gaaacgagcg gcgtcgcctg gacgacctgc
     1621 cgctggtact gggggatcat ctcttcgttc agaatcgtct ccagcacggg gagcacaatt
     1681 tccgacgagg cataaatcca ttcgccataa gccgtcacca tctgctgctc gagcggcgtc
     1741 ttctgcggga tcgcgtcccc cagccgctcg aatttctcaa agccggaatt gccgagcacc
     1801 gggccgatgc ggtcccaggc ggcaagaatt tccggcacca gcggctcggc gtggcgatcg
     1861 cgcccttcgc ggaagaacgc ggtcaaggcc atcacctcgc acagcatgtg gttcgagaag
     1921 gccagcgagt tcatcccgcg ggcgagcacc acgccaccgg aataagtcga ggcatcggcg
     1981 gcattttgca acttgacctt gctgtcaatc tgccgctccg tgttgatgac catgcccagg
     2041 agcatcgtca agagcagcat ggcgaagacg gtcgcgaagc tgatcgagcc gcgctcgtcc
     2101 ttgtgcaagt ccaaggtcca aagtccaagg tccaaggtca gagcgcggcg cgcgtgtcga
     2161 gcgccgggtc cgactttgga ctttggactt tggactttgg acttaatcga catacacgta
     2221 cggcctcacc gatttttccc cctcgcccac catcgtcgcg gtcgcggaga gcggaacgta
     2281 gtacacgccg gcttgctgca gaacctgagg gctgatgcgg tcgggatagc ggctttcgtt
     2341 ggcgcggcga gccagcaggc ggccggggcc ggggagcagg gcgaatttgt gcgtcaccgt
     2401 cacgcgaatc tggtcccgcc agccgatctc gccgggcttg aattcactat cgcggtgata
     2461 cggcggatac tcgatgagcg gcggctcgtc attgcggtgg aaagtatgaa tctcgatctc
     2521 ggtggcggca ttcgcatagg cccacttatt ggccagtcgc tgcggaatgc ggccgttgct
     2581 catcgcggct ggggcgtagg aagcgtagag tgcctgaatg gagccggtca tcgggtcgcc
     2641 gcccgcgatg ccaatatcgc gcgagggagc gatcgggagg caagccagcg ccgcggctgt
     2701 gcggatttgc tcgtacttgg gtgtgcccgg ctcgatcaag tactcagagc catacgcgta
     2761 gctgccaatc agcacccggt tgccgatccg gttctcgcca acctgctcga tcggctccat
     2821 ccgggccgga atccacactg tcgccgaccg cgccgctgca taagccgcat agtggacgat
     2881 catcaatccg atcatgagtt gcgtgatctg cacggccagg agcattagca tcacgaacac
     2941 gggcgcggcg agcacgaagc tgacgctctg cacgccgccc acctcgtcgc ggtgcaggcg
     3001 ggcgagaatc tgccgccgcc actcgggtcg cagcgagcgg gccagcactg ccagggcggc
     3061 cgcggtcgcc gccagcgcca aggcaaaggg ccacagatcg agcagcacgc cgcggagcat
     3121 tgcctcgcct ttcacatggg ccattgccag ggaacgaacg tcgcgatcag ggccgctgcc
     3181 gcacagggac cgagaaagac cggtagttta agcgtttctt gttctttttc cggcggacgc
     3241 agccagatgc cgagcgtaag aacgcccacc agcaactgcc agccgcggag caggagggcg
     3301 agcggtccca gccgccagat caaaatcacc agccccacgc agccgccgaa gataaacgtc
     3361 cagagcagca cttcgagccc cttattcaag cccgccaagg ccccaatcat tgccagcagc
     3421 ttcacgtcgc cgccgccgat cggaaagacg acaaagcaac acaccaccac cagaccgcag
     3481 accagaaatc cgccgaccgc ttcgctcacc gggagccagc cgaccagcgg ctgccagtcg
     3541 cgggcc
//
LOCUS       DASUGB010000355        10434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1985740, whole genome shotgun sequence.
ACCESSION   DASUGB010000355 DASUGB010000000
VERSION     DASUGB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985740"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            647..1075
                     /locus_tag="VFV87_06840"
     CDS             647..1075
                     /locus_tag="VFV87_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5103509.1"
                     /translation="MFVRDILHTKGLTVHSCKPEDTLADAVDLLVGHNIGSLVVLDGD
                     GQMVGIITERDILRASAVTRGPLEFVRVHERMTRCPVVASPDEEVSDTMGVMTERRIR
                     HLPVVENGRLAGIISIGDVVKAQHDELSRENHYLKSYIQS"
     gene            complement(1158..2504)
                     /locus_tag="VFV87_06845"
     CDS             complement(1158..2504)
                     /locus_tag="VFV87_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103510.1"
                     /translation="MPHHFCDGLPRRDFVRLGLAGLFGSSLSLPQLLQAEATPAGKAI
                     KDRGLSVIYLFLKGGLSTIDTLDMKPDAPAEFRGEFDPAPTNVPGIQICSLLPNLAQQ
                     MDKSALLRGFWHKNSDHGPADHYMLTGYFPQAGFNPNLNPNNVRPSLGSIISKKLGQG
                     AARRAAAVPPYVCLPQAHNSAGPAYLGATCAPLSVDADPAAPDFAVPDMAPPPSLAAD
                     RLANRRELLKRVDQLQQSADLEANSKAQTVNVFRQRAFDLMLSPEAKQAFNIHAEPEK
                     LRDAYGRNTLGQSCLMARRLVEAGVRCVTVEHTNWDTHDGNFGTLQTTLLPQLDPGLA
                     TLIRDLSERGLLDKTLVVVTGEFGRTPRINKNAGRDHWGPSFTVLLAGGGVQGGRAIG
                     ASDERAERPADQPHGPEDLFATICHLMGLDPEEEFYTPEGRPVKVVNDGKVIRGLM"
     gene            complement(2580..4760)
                     /locus_tag="VFV87_06850"
     CDS             complement(2580..4760)
                     /locus_tag="VFV87_06850"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5103511.1"
                     /translation="MSLRLASFALGCAASVAAGALPARADERRLNFENDIVPILSRYG
                     CNASGCHGKAEGQNGFKLSVFGFDPRADHRALTVEGRGRRVFPAAPEKSLLLLKASGG
                     MPHGGGVRIPPERPEYATLQRWIAQGMPFGSADDPTIVKIELSTRETTLAMGQSLPLA
                     VTATWSNGRIEDVTSLARFQSNNDGRAAVDEAGLVTAGQSPGVAAVMASYLGQVDALH
                     VVVPRSEPVDPYPMVEEFNFIDGHVYRRLKQLQIVPAEVCSDAEFLRRVYLDLIGTLP
                     TSDEARAFLADGRTNRRALLVEQLLQRPEFADYWALKWADLLRVDRQALGHKGAYDFY
                     RWIHESLATAKPLDVMAREIVTAEGPLAETPPANFYSAVREPGKAASTVSQVFLGVRI
                     ECAQCHHHPFDRWSQEDYFGMTAYFAQLQRKSTPLGEILLAEGDPATTHPRTGQAIPA
                     HALAQPMREKNPAGDRRQELAAWLTAPENPWFARNIANRVWAHLLGRGLVEPVDDFRA
                     TNPPSNPELLNALAAHLASQRFDLRSLLRAVCASRTYQHASHTNETNAKDEQNYSRAL
                     YKRMDAEVLLDALCQTTGVPDQFDGAPAGTRAIELWDSQVEHYFLRLFGRPVRQTACE
                     CERVVEPSVAQVLHLLNSERVDGKLRHEAGRVAELAQSETSDQRLVEELYLTALSRYP
                     SDAERNTGVEYLAAAALGDVSQRRARAEDLLWTLLNSLEFVFNH"
     gene            4984..6489
                     /locus_tag="VFV87_06855"
     CDS             4984..6489
                     /locus_tag="VFV87_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103512.1"
                     /translation="MTIVHCPRCRDEVTVPAKASPRALVRCPLCLEEYLLSEALAELP
                     PTLLVVGGELEEEPELVGAAAATAEAGGEYGLAGEFGGGVFDSSAPAGAMVTPARPGV
                     KGARPKRKERSALGEMIKIVLGGVIGLSLGLVVIWWLARRDPFNLGPKVAPYAPWIVP
                     AEFRGTQPKADDTVQNQSQGSGSKLNAPSPQTSVDPNQQAAKPKAKGNQLQTLPDVLG
                     SEPETPNPLAPASIDLPSIDPFPAARNSPEPELAPEVPLNPSAGDTKAKSKSKAKAAP
                     SEPPEIDLTGLLPDGDTPISTPADSAAPAPLPPIAGGEATPTATAASLASAVQAANDA
                     LGNLEAGKEESKEVRQQLFTDFFLAVADAGRVISHLDPADADVADPLAQLQRLLGELA
                     AQPGKVSAFGHLGRTHLPLRKAGEGFACAGKVIEYRVAGSVFETTLDLGGDVRVLVVS
                     PGNPQDFCEIGDQLLLAGRIVDEPQRNIRGYEGEAERVVMLGDAALVPKAE"
     gene            complement(6504..7526)
                     /locus_tag="VFV87_06860"
     CDS             complement(6504..7526)
                     /locus_tag="VFV87_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5103513.1"
                     /translation="MRKLRVLVLMHEDLVPPDSLEGYTEKEILPWKSEFDVVSTLRKI
                     GHDVRPLGVWDDLGKIRKEIEEWKPHVAFNLLEEFHGVAVYDQHVVSYLELLRQPYTG
                     CNPRGLMLSHDKALCKYILTSHRIRTPKFVVYERGRAVKPTRRIAYPLLVKSVSDDAS
                     LGIAQASLVSSDEKLKDRVEFIHENVQADALVEEYIEGRELYVGLIGNQRVRNLPIWE
                     LLFTKSDDKPLIATAKVKWDSAYQKKLGVETRACTDLPDGVAAAAIRQCRRTYRALSL
                     SGCARVDLRLAADGQVYVLEANPNPNLSRDEDFAESAAKAGLKYPALLQRLLTLGLNY
                     KAAWRL"
     gene            complement(7523..8581)
                     /locus_tag="VFV87_06865"
     CDS             complement(7523..8581)
                     /locus_tag="VFV87_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX5103514.1"
                     /translation="MPRQPRRRAASRRRRKEPEWADYTDDELLQLRFCDLGLKMEGTV
                     LEGRIERLHEELEQRGIEFRPHVWISDEWFSPDGIPGVAIPFYLAHPRLMRLERTQML
                     EVEGGSEDWCMKILRHETGHAIDTAYRLHYRKHYRTLFGKFTTEYPKTYQPRPYSKSF
                     VLHLEPSYAQAHPAEDFAETFAVWLKPRSGWRTQYEGWPVLKKLEYVATLMEEIRGKK
                     PKVHSREHVDPVGKLRKTLASHYEEKRKLYGTPHPNFYDQHLLKLFSNGPECARNPSA
                     AAFLGRVRADLRKTVAHWTGEYQYTIDQVIAEMIDRCRELNLRLDRPFRQARRDALVL
                     VTVQTMNYLHGGHHRVAL"
     gene            8849..10138
                     /locus_tag="VFV87_06870"
     CDS             8849..10138
                     /locus_tag="VFV87_06870"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5103515.1"
                     /translation="MRNVISLVLGGGRGTRLYPLTKYRSKPAVPLAGKYRLIDIPLSN
                     CINSEMTRIYVLTQFLSVSLHRHIRQTYRFDAFSGGFVEILAAQQMTDDARADWYQGT
                     ADAVRKQLRYIMEPGIDYVVILSGDQLYRMDYGDMLRTHIESKADATIAGIPVTREQA
                     KAFGIMRINDEGRVQGFLEKPQTSKEADIVRMEPSWIDARGIPSNGRDLIASMGIYVF
                     NRDTLIDSLLKTDYMDFGKEVFPASVRAKNVQLHLFDGYWEDIGTIRAFYEANLSLAS
                     AEPPFDFHTAAAPIYSRARFLPPTLVEDAVVKRSLVADGCRIGKGSVIENSVVGLRCR
                     IGENVTIKNTVIMGADYFDDDFRAQAAARGGPPLGIGAGSHIEGAILDKNCRIGKSVR
                     IINEQKTETRGEDEACIIREGIPNVVKEGVLPDGWKL"
     gene            complement(10195..>10434)
                     /locus_tag="VFV87_06875"
     CDS             complement(10195..>10434)
                     /locus_tag="VFV87_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5103516.1"
                     /translation="GSVGGTYSTPIINVPEVAILLVGRSRKMPVVVDDEIAIRLMMPL
                     SLAYDHRLVDGATAARFLNDVIDYLEAPSRLLLAP"
BASE COUNT         1841 a         3282 c         3422 g         1889 t
ORIGIN      
        1 gagaagatcg ccgaattgct ggcggaaaat gccgcgtaga ttgaccgcga accctgccgc
       61 cacgccaact gtcactggat atcgcgactt cggacgggat tgagagagct gcaatttcct
      121 cttgattttt ccgggcctcg aagcaattat gcaggtgacg tgtcgcgctc cgcggcgcga
      181 cttgactagc aagctcgcag tacgccgcat cttcggcttc gcggcggagg cggggtaact
      241 tctagtggaa aggaggtggt ctagtgagtt ttgtctgtac cagccggggt ggcgcctgct
      301 gacagccggc gggctgcttc tggcagccac caacccctcg tcacagatca ccgatcgttg
      361 acgagctacc ttggggcccg gcctcccgcg tggaggccgg gcctttttcg ttttgggaag
      421 ctcgccggcg tcgcggagct tgtgaatcaa ttcacaaggt cgcgcccgaa atcgccacaa
      481 ccagctctaa aaaacatttg actccggcgc gctcactgtt ctaatttgta ggttcactgt
      541 cggcatcctt ttcgcagggt tcttatcgcc ggcagatcag agaagagccg cttgtttttt
      601 gttgtcctgg tccgttcact gtctccatca agcggagcct gttgtcatgt tcgttcgcga
      661 tatcctgcac accaagggcc tcacggtcca cagctgcaag ccggaagaca cgctggccga
      721 tgccgtcgat ctgctcgtgg gtcacaacat cggatcgcta gtcgtgctcg atggtgacgg
      781 tcagatggtg ggcatcatta cggaacgcga tatcttgcgg gccagcgccg ttacgcgcgg
      841 gccgctcgaa ttcgtccgcg ttcacgagcg gatgacccgc tgcccggtcg tcgcttcgcc
      901 ggacgaggaa gtcagcgaca ccatgggggt gatgaccgag cgccgcatcc gccatctgcc
      961 ggttgtggaa aacggccgcc tggcggggat catttcgatc ggcgacgtcg tgaaagccca
     1021 gcacgacgag ttgtcgcgcg agaatcacta cctgaagagc tatattcagt cgtaagtggg
     1081 gcaggcttcc agcctgccat aaatcggtct cgcagcggac tcgcgcagcg cttcaggttc
     1141 cgcccggcag gcgggaccta catcagcccg cggatcactt tcccgtcgtt gacgaccttc
     1201 accggccgcc cttcgggcgt gtagaactct tcctcggggt ccaggcccat caggtggcag
     1261 atcgtggcga agaggtcctc cggcccgtgc ggctgatcgg cgggacgctc ggcccgctcg
     1321 tcgctggcgc cgatcgcgcg gccgccttgc acgccgccgc cggcgagcag caccgtgaac
     1381 gacggccccc agtggtcgcg gccggcattc ttgttgatcc gcggcgtgcg gccgaattcg
     1441 cccgtgacga cgaccagcgt cttgtcgagc aggccgcgct cggacagatc gcggatcagc
     1501 gtcgccaggc cgggatcgag ctgcggcagg agcgtcgtct gcagcgtgcc aaagttgccg
     1561 tcgtgcgtgt cccagttcgt gtgctcgacg gtcacgcacc gcacccccgc ctcaaccagc
     1621 cgccgggcca tcaggcacga ctggccgagc gtattgcggc cgtaggcatc gcgaagcttc
     1681 tcgggctcgg cgtggatgtt gaacgcctgc ttggcctcgg gcgagagcat caggtcgaag
     1741 gcccgctggc ggaagacgtt gaccgtttgg gccttactgt tggcttcgag gtccgccgat
     1801 tgctggagtt gatcgacgcg cttgagcagc tcgcggcggt tggccaggcg gtcggcggcc
     1861 aggctcggcg gcggggccat gtcgggaacc gcaaaatcgg gcgcagcggg atcggcatcg
     1921 accgagagcg gggcgcaggt cgcaccgaga taagccggcc cggcgctgtt gtgggcctgc
     1981 ggcaggcaga catacggcgg cacggcggct gcccggcggg cggcgccctg gccgagcttt
     2041 ttgctgatga tcgagcccag gctgggccgc acgttgttcg ggttcaggtt cggattgaac
     2101 ccagcctgcg ggaaatagcc ggtcagcatg tagtgatcgg ccggcccgtg gtcgctgttc
     2161 ttgtgccaga agccgcggag cagggcagac ttgtccatct gctgggccag gttcggcagc
     2221 aggctgcaga tctggatgcc cggcacgttg gtcggcgccg gatcgaattc gccgcgaaac
     2281 tcagccgggg catccggctt catgtcgagc gtgtcgatcg tcgagagtcc gcccttgagg
     2341 aagagataaa tgaccgacag gccgcgatcc ttgattgcct tgcccgcggg cgtggcctct
     2401 gcctgcaaaa gctgcggcag cgagagcgac gagccgaaca agccggcgag accgagacgg
     2461 acgaagtcgc gccgtggcag gccgtcgcag aagtggtgag gcatcgtcgg gctccgtggg
     2521 gcagacattc ttgtctgccg cgaaagtttc ttggcaggca agaatgcctg ccccacttct
     2581 cagtggttga acacaaactc cagcgagttc agcagcgtcc aaagcaggtc ttcggcccgg
     2641 gcgcggcgct ggctcacatc gcccagcgct gcggcagcca gatattctac acccgtattg
     2701 cgctccgcat cggacggata ccggctgagg gcagtcaggt acagctcctc gaccagccgc
     2761 tggtcgctcg tttcgctttg ggccagctca gcgacgcgtc cggcttcgtg ccgcagcttg
     2821 ccatcgaccc gctcggaatt gagcaggtgc agcacttggg cgacgctcgg ctcgacgacc
     2881 cgctcgcact cgcaagctgt ctgccgcacc ggccgcccaa acagccgcag gaaatagtgc
     2941 tcgacctggc tgtcccagag ctcgatggcc cgcgtgccgg ccggcgcgcc gtcaaactgg
     3001 tcgggcacgc cggtcgtctg gcaaagggcg tcgagcagca cctcggcgtc catccgcttg
     3061 tagagcgccc gagagtagtt ctgttcgtct ttagcgttcg tttcgttggt atgcgatgcg
     3121 tgctgatacg tccgcgaagc acaaaccgcc cgcagcaagc tgcggaggtc gaacctctga
     3181 ctggccaggt gcgccgccag cgcattgaga agctcaggat tgctcggcgg attggtcgcc
     3241 cggaaatcat cgaccggctc tacgagcccg cggccgagca aatgcgccca gacgcggttg
     3301 gcgatgttcc gagcgaacca cggattttcc ggcgcggtca gccacgcagc cagctcctgg
     3361 cggcggtcgc ccgcgggatt cttctcccgc attggctggg ccaaggcatg cgcggggatc
     3421 gcctgtccgg ttcgcggatg cgttgtcgcg ggatcgccct cagccagcag gatctcgccc
     3481 agcggcgtgc tcttccgctg aagctgggcg aaataagccg tcatgccgaa ataatcttcc
     3541 tggctccagc ggtcgaacgg atggtggtgg cactgggcgc actcgatccg cacgcccagg
     3601 aacacctgcg agaccgtgct cgccgctttg ccgggctcgc ggaccgcgga ataaaagttg
     3661 gccggcggcg tttccgccag cgggccttcg gcggtgacga tctcgcgggc catcacgtcc
     3721 agcggcttgg ccgtcgcgag cgattcgtga atccagcgat aaaagtcgta cgcgcccttg
     3781 tgaccgagcg cctgccgatc gacgcgcagc agatcggccc acttcagggc ccagtaatcg
     3841 gcaaactccg gccgctgcag gagctgctcg accaggagcg cgcggcgatt ggtccgcccg
     3901 tcggccagga aggcccgcgc ctcgtcggaa gtcggcagcg tgccgatcag gtcgagatac
     3961 acgcgccgta ggaattccgc gtcgctgcac acctcggccg gcacaatctg aagctgcttc
     4021 agccggcgat agacgtggcc gtcgataaag ttgaactcct cgaccatcgg atagggatcg
     4081 acgggctccg agcgcggtac gacaacgtgc agcgcatcga cctggcccag gtaactggcc
     4141 atgaccgcgg cgacgcccgg cgactggccc gccgtgacca ggcccgcttc atcgaccgcc
     4201 gcccggccgt cgttgttcga ctgaaaacgg gccaggctcg tcacgtcctc gatgcggcca
     4261 ttggaccacg tggccgtgac ggccagcggc agcgactggc ccatcgccag cgttgtttcc
     4321 cgcgtcgaaa gctcgatctt gacgatcgtc ggatcatcag ccgagccgaa cggcattccc
     4381 tgcgcgatcc accgctggag cgtagcgtat tcgggacgct ccggcggaat tcgcacgcca
     4441 ccgccgtgcg gcatgccgcc gctggctttc aagagcagga ggcttttctc gggtgcggcc
     4501 ggaaaaaccc gccggccgcg gccttcgacg gtcaaggccc ggtggtccgc ccgcggatcg
     4561 aagccaaaga ccgagagctt gaagccgttc tgcccctccg ccttcccatg gcagcccgag
     4621 gcgttgcagc cataccggct gaggatcggg acgatgtcgt tctcaaaatt cagccgcctc
     4681 tcgtccgccc gcgcaggcag agcgccagcc gcaacactgg cggcgcagcc caaggcaaag
     4741 ctcgcaaggc gcaacgacat cggcacaggc gggagcgtgg ttcaggcggg aagaacgtac
     4801 cacaagccga tcgaatcaat ggatgttaat gcaatccagg cgggccgtgc aagcagaaag
     4861 ttccgaaacg ctgcgggtcg cgcgacgccg cgattggccc ttctgctagc ccgtgggtaa
     4921 tgcgtcaagt tacactatcg ttcgtcgatg tgaatgtcga atgacgcggg gagcttgctg
     4981 aagatgacca ttgtccactg cccgcggtgt cgggatgaag tgaccgtgcc ggcgaaggct
     5041 tcgccgcggg cgctggtgcg ctgtccgctg tgcctggagg agtatctgct ctccgaggcg
     5101 ctggccgagc tgccgccgac gctgctggtt gtgggcggcg agttggagga agagcccgag
     5161 ttggtcggcg ctgcggcggc gacggcggag gcgggcggcg agtacggtct tgcgggcgag
     5221 ttcggcggcg gcgtcttcga ttcgtcggcg cctgcggggg cgatggtcac gccggccagg
     5281 cccggcgtca agggagcgcg gcccaagcgt aaagagagga gcgccttggg cgaaatgatc
     5341 aagattgttc tcggcggcgt gatcggcttg tcgttggggc tcgtggtgat ctggtggctg
     5401 gcgcggcgcg atccgttcaa tctcggcccg aaggtcgccc cgtatgctcc ctggattgtc
     5461 cccgcggaat ttcgcggaac tcagccgaag gccgacgaca cggtgcagaa ccaaagccaa
     5521 ggcagcggat cgaagctgaa cgcgccatcc ccgcagacat cggtcgatcc gaatcaacaa
     5581 gccgcaaagc ccaaggcgaa agggaaccaa ctgcaaacgc tgcccgacgt gctgggcagc
     5641 gagccggaaa cacccaatcc gctcgcgccg gcatcgattg atttgccgtc gatcgatcca
     5701 ttcccggccg ccaggaattc acccgagccg gaacttgctc cagaagtgcc actcaatccg
     5761 agcgcgggcg acaccaaggc gaagtcgaaa agcaaggcga aagctgcccc aagtgagccg
     5821 cccgagatcg acttgacggg tttgctgccc gacggcgata cgccgatatc cacgccggct
     5881 gattctgcag caccggcgcc gctgccgccg attgccggcg gcgaggcgac gccgacggcc
     5941 actgccgcca gtcttgcatc ggccgtgcaa gctgccaacg acgccctggg caacctggag
     6001 gcgggcaagg aggaatcgaa ggaagtccgc cagcagctct ttaccgactt ttttctggcg
     6061 gtggccgatg cggggcgagt aatttcgcac cttgatccgg ccgacgccga cgtggccgac
     6121 ccgctggctc agctccagcg gctgctgggc gaattggccg cgcagccggg caaggtgagc
     6181 gccttcgggc accttggccg cacccacctg cctttgcgca aggcgggcga agggttcgcg
     6241 tgtgccggca aggtgatcga ataccgcgtg gccggcagcg tgtttgagac gacgcttgac
     6301 ttgggcggcg acgttcgcgt gctcgttgtt tcgcccggca atccgcagga tttctgcgag
     6361 atcggcgatc aactgctcct ggccgggcgg atcgtcgatg agccgcagag gaatatccgc
     6421 ggctatgaag gcgaggcgga gcgggtcgtc atgctgggcg atgcggcgct ggtgccgaag
     6481 gccgagtgat cgagcggccc tgatcacaac cgccaggcgg ccttgtagtt caggccgagc
     6541 gtgagcaatc gctgcagcag cgccggatac ttgagccccg ccttggcggc cgattcggcg
     6601 aagtcttcgt cgcgggagag attcgggttg gggttggctt ccagcacata cacctgcccg
     6661 tcggccgcca gtcgcaggtc cacccgggca cagccgctga gggacaaggc gcgatacgtg
     6721 cggcggcact gccggatggc ggcggcggcg acgccgtcag gcagatcggt gcaggcgcgc
     6781 gtttcaactc ccagcttttt ctgataggcg gaatcccact tcaccttggc ggtggcgatg
     6841 agcggcttgt cgtcggactt cgtgaacagc aactcccaga tcggaaggtt ccgcacgcgc
     6901 tggttcccga tcaggcctac gtacagctcg cggccctcga tatactcctc gacgagcgca
     6961 tcggcctgga cattttcgtg gatgaactcc acgcggtctt tcagcttctc gtcgcttgag
     7021 accagcgagg cctgggcgat gcccagcgag gcatcgtcac tcaccgattt gacgagcaag
     7081 ggataggcga ttcgcctggt cggcttgacg gcccggccgc gctcatagac cacaaacttg
     7141 ggcgttcgga tgcggtggct cgtcaggatg tatttgcaca aggccttgtc gtgcgagagc
     7201 atcagccccc gcggattgca gccggtatag ggctgccgca gcagttccag atagctgacg
     7261 acgtgctggt catagacggc gacgccgtgg aattcttcga ggagattgaa ggcgacgtgc
     7321 ggcttccact cttcgatctc tttgcggatc ttacccagat catcccagac gcctaacggc
     7381 cgcacgtcgt gcccgatctt ccgcagggtg ctgaccacgt cgaattccga tttccacggc
     7441 agaatctcct tctcggtgta accctcgagg ctgtcggggg gcaccagatc ttcgtgcatc
     7501 aggaccagca cgcgaagttt tctcatagcg ccacgcggtg atggccgccg tgcaggtagt
     7561 tcatggtttg caccgtcacc agcaccaggg cgtcgcgccg ggcctggcgg aacggccggt
     7621 cgagccgcag gttaagctca cggcagcgat cgatcatctc ggcgatcacc tggtcgatcg
     7681 tgtattgata ctcgccagtc caatgggcga cggtcttccg cagatcggcc cgcacgcgcc
     7741 ccaagaaggc ggcggccgat ggattgcggg cgcattccgg accattggaa aacaacttca
     7801 ataaatgctg atcatagaaa tttgggtgcg gcgtgccata caacttgcgc ttttcttcgt
     7861 agtgtgaagc cagcgtcttg cgcagcttgc ccaccggatc gacgtgctcg cgcgaatgca
     7921 ctttcggctt tttgccgcga atctcctcca tgagcgtcgc gacgtactcc agcttcttga
     7981 gcaccggcca gccttcgtat tgcgtccgcc agccggaccg cggcttgagc cagacggcga
     8041 acgtctcggc gaagtcctcg gcgggatgcg cctgcgcgta ggagggctcc aagtgcagga
     8101 cgaagctttt gctgtagggc cgcggctgat aggtcttggg atattccgtc gtgaacttgc
     8161 cgaacagcgt gcggtagtgc ttgcgatagt ggagtcggta agcggtgtcg atggcgtgcc
     8221 ccgtttcgtg gcggaggatc ttcatgcacc agtcctccga tcccccttcc acctccagca
     8281 tctgcgtgcg ctccaagcgc atcagccggg gatgggccag ataaaacggg atggccacgc
     8341 ccgggatgcc atcgggcgag aaccactcgt cggagatcca gacgtggggg cggaactcga
     8401 tcccgcgctg ctcgagctcc tcgtgcaagc gctcgatgcg cccttccagc acggtgcctt
     8461 ccatcttcag gcccaggtcg cagaaccgca gctgcagcag ttcgtcatcc gtgtagtcgg
     8521 cccattcggg ttcttttctg cggcggcgcg aagcggcccg ccggcgcggt tgtctgggca
     8581 ttgcgtagta caagtacact atgggcgagg aagacgcgaa ggccgtctcc tcatcttgac
     8641 tggccattgg aaaattgcca gtgcgtccct ggggccagcg gcgcagatca ggcctgaaac
     8701 agcgccggtt gcgatggttg cttcgggtgg ggtatcttag ggccatgtcc agtggcccat
     8761 gtttggggta catggctgcc cgcaggtctc atcgggcggc gggttctgct cccttgatcc
     8821 tggcccttca cgaaagagtc ggctttccat gcgcaacgtc atttcgctcg tcctgggcgg
     8881 aggccgggga acgcggctgt acccgctcac caagtaccgc agcaagccgg cggtgccgct
     8941 ggcggggaaa tatcgcctga tcgacatccc gctgtcgaac tgcatcaaca gcgaaatgac
     9001 gcggatttac gtgcttacgc agttcctctc ggtgagcctg caccggcaca tccgccagac
     9061 ctaccgcttc gacgccttca gcggcggctt cgtcgagatc ctggcggccc agcagatgac
     9121 cgacgacgcc cgggccgact ggtatcaagg cacggccgac gccgtccgca agcagctccg
     9181 ctacatcatg gagccgggca tcgactacgt cgtcatcctc tccggcgacc agctctaccg
     9241 tatggattac ggcgacatgc tccggacgca catcgaatcg aaagccgacg ccacgatcgc
     9301 cggcattccc gtcacccgcg agcaggccaa ggcgttcggc atcatgcgga tcaacgacga
     9361 ggggcgcgtg caaggctttc tcgaaaagcc ccaaacgagc aaagaagccg acatcgtccg
     9421 catggagccc tcgtggatcg acgctcgcgg cattcccagc aacgggcgcg acctgatcgc
     9481 cagcatgggc atttatgtct tcaaccgcga cacgttgatc gactcgctgc tgaagactga
     9541 ttacatggat tttggcaagg aggtctttcc cgcgtcggtg cgggcgaaga acgtgcagct
     9601 ccacctgttc gacggctact gggaggacat tggcacgatt cgagcgtttt acgaggcgaa
     9661 cctcagcctg gccagcgccg agccgccctt cgattttcac accgccgccg cgcccatcta
     9721 cagccgggcc cgcttcctgc cgccgacctt ggtcgaggac gcggtcgtca aacggagcct
     9781 ggtcgccgac ggctgccgga ttggcaaggg gagcgtgatc gaaaacagcg tcgtcggtct
     9841 gcggtgccgc atcggcgaaa acgtgacgat caagaatacg gtgatcatgg gggccgacta
     9901 tttcgacgac gatttccgcg cccaggccgc cgcccgcggc ggcccaccgc tgggcatcgg
     9961 cgccggcagc cacatcgaag gggcgatcct cgacaagaac tgccgcatcg gcaagagcgt
    10021 gcggattatc aacgagcaga agaccgaaac ccgcggcgag gacgaagcct gcatcatccg
    10081 cgaaggcatc cccaacgtcg tcaaggaagg cgtgctgccg gatgggtgga agctgtaacc
    10141 ttggtagtca tcagccttgg tagtcatcac gctccgcgtg ctgcctacca tgcgttacgg
    10201 cgccagcagc aggcggctgg gcgcttcgag gtagtcgatc acgtcgttga gaaaacgtgc
    10261 tgcagtggcg ccgtcgacga ggcggtgatc gtaggcgagt gacagcggca tcatcaggcg
    10321 gatggcgatt tcgtcgtcaa cgaccaccgg catcttgcgc gagcggccga cgaggaggat
    10381 cgctacttca ggtacattga tgattggcgt gctataggtt ccgccgacgg aacc
//
LOCUS       DASUGB010000356         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_874785, whole genome shotgun sequence.
ACCESSION   DASUGB010000356 DASUGB010000000
VERSION     DASUGB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874785"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1080
                     /locus_tag="VFV87_06880"
     CDS             82..1080
                     /locus_tag="VFV87_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5103517.1"
                     /translation="MPDPLLRVEDLHVHFRTDDGLVRAVNGVSFDLAAGETLGIVGES
                     GSGKTVTNLALLGLIPQPPGIIERGQALYRGQDLLKLSASQLAQIRGRRIAMIFQDPM
                     TALNPFLTVGEQLTEVTRLHLKHSAAEARKHAIAMLAKVGIPAPDRRIDDYPHQFSGG
                     MRQRVMIAMALSCQPDLLIADEPTTALDVTIQAQMLELIKELQTTQGTAVILITHSLG
                     IVASVCDRVIVMYAGQIAEEAAVGPLFERPQHPYTLGLLQSIPRWDASTHEPLKAIAG
                     QPPDMLHPPSGCPFHPRCPFVLERCPHEMPPLEKAVHGGRKACWANLTCSAATVSS"
     gene            1116..1331
                     /locus_tag="VFV87_06885"
     CDS             1116..1331
                     /locus_tag="VFV87_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103518.1"
                     /translation="MKAFEFLTHPTADGSIQLPLDVSRQVDASQEIKVIVMLPDDEDQ
                     AWNRFGMEQFFKGYAPSDNIYDDLRAG"
     gene            1309..1638
                     /locus_tag="VFV87_06890"
     CDS             1309..1638
                     /locus_tag="VFV87_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5103519.1"
                     /translation="MTTYAPGEIVLVDFPFVSGAAAALRPALVVLDTGDSDVVLARIT
                     TKSHTSGHDVAIADWRGAGLRAASFVRLHKLATAEKPTVFKRLGSLQPPDHQRVAAIL
                     KGMFNSW"
     gene            1954..2982
                     /locus_tag="VFV87_06895"
     CDS             1954..2982
                     /locus_tag="VFV87_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5103520.1"
                     /translation="MSVPATESSNRINVRLAPSGKPLLEVRDLKVHFPVRGGLFGAAR
                     VIRAVDGVSFDIRPGETLGLVGESGCGKTTTARAIVGLLERSVMQGEVWLDGYRIDHL
                     SRAAMLPHRRKVQMIFQDPYASLNPRMTVGSIIAEPMQIFGLHARRERKLEVMRLMDL
                     VGLNPRLLNRYPHEFSGGQRQRIGIARALAVEPRLIVCDEPVSALDVSIQAQVINLLV
                     DLQQQLGVAYLFISHDLSVVRHISHRIGVMYLGRIVELAAADELHERPLHPYTQALLS
                     AAPIPEPAAERRRRRIALQGEVPSPDREYPGCPFADRCPIAEPYCREVVPRLEGAGHL
                     VSCHKAER"
     gene            3008..4357
                     /locus_tag="VFV87_06900"
     CDS             3008..4357
                     /locus_tag="VFV87_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5103521.1"
                     /translation="MFGWFKTKCPLATAEKVWVESSMDWLAGTLGIERLIEAELIEPS
                     DEFFPDHYSGQDADAARIFKQVCGLMQIDPAQVRLELCDRIGDDKEDKIVGQYVSGDP
                     ATIVVRRSQLADPESLVATLAHELAHEILLGGGFLEENNEDTERLTDLTTVFLGLGIF
                     GANSFLREATVREGRFSTWSIRKQGYLPIRMYGYGLALFAWARDESDPDWAAHLRADA
                     REPFTKGLQFLLETDDSVFRLVDPERHASDEQRFDAAVANLAHRSPSRRMWGLTHLLE
                     SNSHAATAIEPIGKLLHDRDTDVRGTAAYVLGTIGPPAAEHVPRLQDLLTSRDSLSRE
                     GAAYALGQIRPDDPIVIDDLARVLDDEDARVLAAAASALSNYGPAAAAALPYLLKRYE
                     KSLIRCDYARIDALAAALRAVAPDAIHRVRDHFASDAELRQYAEDCLHESTPSAEPE"
     gene            4386..>4726
                     /locus_tag="VFV87_06905"
     CDS             4386..>4726
                     /locus_tag="VFV87_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103522.1"
                     /translation="MNRQYVVCFVFGLTAACLADDASNARRPKNDEELRFWLDNMLVH
                     HQFTVEEAAAATGLSADEVTAAAKRFGIDAGRRPERAADQPLLVLPYPGGRHPRIGFL
                     DGAVDPQRETKV"
BASE COUNT          849 a         1533 c         1482 g          862 t
ORIGIN      
        1 ggctcgtccg cggcgcagca ggagcgcgtc cgatctccta gaatcccaaa gcggtaacaa
       61 gttcccgcac tccaattcaa gatgcccgac ccgctgctcc gcgtcgaaga cctccacgtc
      121 cacttccgca ccgacgacgg cctggtgcgg gcggtcaacg gcgtgtcgtt cgacctggcg
      181 gcgggcgaaa cgctgggcat cgtcggcgaa tcggggagcg gcaagacggt gacgaacctc
      241 gccctgctcg gcctcatccc gcagccgccg ggaatcatcg agcgcggcca ggccctctat
      301 cgcgggcagg atttgctgaa gctcagcgcc agccagctcg cccagattcg cgggcgccgg
      361 atcgcaatga tctttcagga cccgatgacg gccctcaatc cgttcctgac cgtcggcgag
      421 caactcaccg aagtcacgcg gctgcatctg aagcattccg cggccgaggc ccgcaagcac
      481 gcgatcgcta tgctcgccaa ggtcggcatt cccgcgccgg accggcggat cgacgactac
      541 ccgcaccagt tctccggcgg catgcggcag cgggtgatga tcgccatggc cctctcgtgc
      601 cagcccgacc tgctcatcgc cgacgagccg acgacggccc tggacgtaac aatccaggcc
      661 cagatgctgg agctgatcaa ggagctgcaa accacgcagg ggacggccgt gatcctgatc
      721 acgcattcgc tgggcatcgt ggccagcgtg tgcgaccggg tgatcgtgat gtacgccggc
      781 cagatcgccg aagaagcggc ggttggtccg ctcttcgagc gcccgcagca cccgtacacg
      841 ctcggccttc tgcaatcgat cccccgctgg gacgcctcca cgcacgagcc gctcaaggcc
      901 atcgccggcc aaccgcccga tatgctgcac ccgcccagcg gctgcccgtt tcatccgcgg
      961 tgccccttcg tcctggaacg ctgcccgcac gaaatgccgc cgctggagaa ggccgtgcat
     1021 ggtgggcgca aggcgtgctg ggcgaatttg acttgtagtg cggcgaccgt ttcgagctaa
     1081 taatacggac agacctcaac ttgtaggaac ggaatatgaa agcctttgag ttcttgaccc
     1141 atccgactgc ggacggatcg attcagttgc ctcttgacgt gtctcgtcag gttgacgctt
     1201 cgcaggaaat caaagtgatc gttatgttgc ctgacgatga agaccaagct tggaatcgct
     1261 ttggcatgga gcagtttttc aaaggctacg caccttctga caacatctat gacgacctac
     1321 gcgccgggtg agatcgtcct cgtcgatttt ccctttgtga gcggcgcggc agcggctctg
     1381 cggccggcgc tcgtcgtgct ggacactggc gacagcgacg tcgtcctcgc gcgcatcaca
     1441 acaaagtcac acacgtcagg tcacgacgtc gcgattgccg actggcgtgg cgcgggcctg
     1501 cgcgccgcgt cgtttgtccg cctgcacaag ctggccacag cggaaaaacc cactgtgttc
     1561 aagcggctgg gcagtcttca accgccagat caccagcgcg tagccgcgat attgaaggga
     1621 atgttcaact cgtggtgaca tcgaatgctc cagatccgat cgctctccga gctgcgcggc
     1681 ggttggagct tgatgcagcc attggattag gagcatcctt tctttgctgt gcacttggac
     1741 tcctccttgg tattcgcaat ttgaccaaag tgaacgctga aatcgagcat tccaagcagc
     1801 acgatattcc agccgatgtc caatcaaggt tgttctccgc tcgcgctgtt agcgtgatcg
     1861 ggattgtcgt gtccattgcg ttgccactcg cctcaattct ctggtccgca attgcatttt
     1921 ctctacgcgg ccaatgaagg aggcaagtcg ccgatgagcg ttccggccac cgaatcgtca
     1981 aatcggatca atgtgcgatt ggcgccatcc ggcaagccgt tgctggaggt gcgcgatctg
     2041 aaggtgcatt tccccgttcg cggcggactc tttggcgcgg cgcgcgtcat tcgggccgtc
     2101 gatggcgtga gcttcgacat ccgcccgggc gagaccctgg gcctcgtggg cgaatcgggc
     2161 tgcggcaaga cgacgaccgc ccgagcgatc gtcggcctgc tggaaaggag cgtgatgcaa
     2221 ggggaggtct ggctcgatgg ctaccgcatc gaccatctct cgcgcgccgc catgctgccg
     2281 caccgccgca aagtgcaaat gatctttcag gatccgtacg cctcgctcaa tccgcggatg
     2341 acggtcggca gcatcatcgc ggagccgatg cagattttcg gcctgcacgc gcgccgcgag
     2401 cgcaagctgg aagtgatgcg gctgatggac ctggtgggtc tcaatccgcg gctgctcaac
     2461 cgctatccgc acgaattctc cggcggccag cggcagcgga tcggcatcgc ccgggccctg
     2521 gccgtcgagc cgcggctgat cgtctgcgac gagccggtgt cggccctcga cgtctcgatc
     2581 caggcccagg tgatcaacct gctcgtcgat ctgcagcagc agctcggcgt cgcgtatctg
     2641 ttcatctcgc acgatctgtc ggtggtgcgg cacatctcgc accggatcgg cgtgatgtat
     2701 ctcgggcgga tcgtggaact ggccgctgcc gatgaattgc acgagcggcc gctgcaccct
     2761 tatacgcagg ccctgctctc cgccgccccc attcccgaac ccgccgccga gcgccgccgg
     2821 cggcggatcg ccctccaggg cgaagtccct tcgcccgacc gcgaataccc cggctgcccc
     2881 ttcgccgacc gctgcccgat cgccgagccc tactgccgcg aagtcgtccc ccgcctggaa
     2941 ggcgccggcc acctggtatc gtgccacaag gcggagcggt aaaaaaagga accgcgaggg
     3001 tcgcctcatg ttcggctggt tcaagacaaa atgcccgctg gccacggcgg agaaggtctg
     3061 ggtcgagagc agcatggatt ggctcgccgg cacgctcggc atcgaacgct tgatcgaggc
     3121 cgaactcatc gagccgagcg acgagttctt tccggatcac tacagcggac aggatgccga
     3181 cgcggcgcga atcttcaagc aagtctgcgg gctgatgcag atcgatccgg cgcaagtgcg
     3241 gctggagctg tgcgatcgga ttggcgacga caaggaagac aagatcgtgg ggcagtacgt
     3301 gtcgggtgat cccgcgacaa tcgtcgttcg ccggtcgcag cttgccgatc ccgaatcgct
     3361 cgtcgccacg ctggcgcacg aactggccca cgagatcctc ctcggcggcg gcttcctgga
     3421 agagaacaac gaggatacgg agcggcttac cgatctgaca acggtctttc tcgggttggg
     3481 aatctttgga gcaaactcgt ttctccgcga agccacggtg cgcgaagggc gtttcagtac
     3541 gtggtccatt cgcaagcaag gatatttgcc catccgcatg tatggctacg gtctggccct
     3601 gttcgcctgg gcgcgcgacg aaagcgaccc cgattgggcc gcgcatttgc gggccgacgc
     3661 gcgcgagcca ttcacgaaag ggctgcaatt ccttctggaa acggacgact cggtctttcg
     3721 gctggtcgat cccgagcggc atgcttccga cgagcagcgc ttcgacgcgg cggtcgcgaa
     3781 cctcgcgcac cgctcgcctt cccgccggat gtgggggctg acgcacctgc tggaaagcaa
     3841 ctcgcatgca gccaccgcga tcgagcccat cggcaagctc ctgcacgatc gcgataccga
     3901 cgtccgcggc acagccgcct acgttctggg aacgatcggc cccccggccg cggagcatgt
     3961 tccgcggctg caggatttgc tcaccagccg cgattcgctc agccgcgagg gtgcggctta
     4021 tgccctcggt cagatccgcc ccgacgatcc gatcgtgatt gacgacctcg cgcgggtgct
     4081 cgacgatgaa gatgcaaggg tccttgcagc ggctgcgagt gcgctcagca actatggacc
     4141 ggctgcagca gctgcattgc cgtacctgct caagcgttat gaaaagtcgc tgatccgctg
     4201 cgactatgcg cgaatcgacg ctttggccgc ggccctgcgc gcggtggccc cagacgcgat
     4261 ccatcgcgtc cgcgatcatt tcgcgtcgga tgcggaactg cggcagtacg ctgaggattg
     4321 cttgcacgaa tcgacgccga gcgcggagcc agaatagcca gccaatacag tgaagggacg
     4381 ttcccatgaa tcgccagtac gttgtctgct tcgttttcgg attgaccgct gcctgcctgg
     4441 ccgacgacgc gtcgaacgcc cgccggccca agaacgacga ggagctgcgg ttctggctcg
     4501 ataacatgtt ggttcaccac cagttcacgg tggaggaagc ggcggcggcg acgggattgt
     4561 cggcggacga agtgacagcg gctgccaagc gcttcgggat cgatgcgggc agacggcccg
     4621 agcgggccgc cgatcagccg ctcctggttc tgccctatcc cggcgggcga catccgcgga
     4681 tcggctttct cgacggagcg gtcgatccgc agcgggagac gaaggt
//
LOCUS       DASUGB010000357         5405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_179469, whole genome shotgun sequence.
ACCESSION   DASUGB010000357 DASUGB010000000
VERSION     DASUGB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179469"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="VFV87_06910"
     CDS             <1..1841
                     /locus_tag="VFV87_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103523.1"
                     /translation="AGCSTRPAITPVKKTEAEQLANRDDLSLALDSLRKLAEGPDPQA
                     EQRTIFYLNQWLASTKPSAGEWKPDRLLTNLPRSLNYTLGMDRLSDLHFSYTIKLPSA
                     LVIAMGGDPENYEVPEIPGTDLPHLQTSLWMHDIAHRAKQTTIPDELAQWLTAHEKQI
                     GIAEAEQLASAERVFDWTIRNIQLDELPPPPKGPAATADARTEPVAPALLGEPGPGYR
                     QLPRDVLVHGHGDAWERARVFILLCRQLQIDTVMLGLIQDQSPVPRGWLPAVLIKDEL
                     YLFDTSLGLPIPGPEGKGIATLSQVLADPRLLRKLDVEGEPPYPVTEKDLKIAALIEA
                     EPDALSHRMQLLQGALPADQHLILTARPSLLEPKLRKCRGIGQISLWQVPFEAAMYAF
                     PLGRARGAAQDPLAAAQLQLELETFNPNLPLAKGRNLHLQGRFEKQDQALGARNHYLA
                     SRPPDQEMQRVESSEIVRKAMGFQQSLPAGEKEQKKEIEFRMTLARTRKHHATYWMGL
                     TYQEAGNQSAAIEWLADRTMEAYPPSPWIPGARYNLARCYEDLGQWEAARKWLLSDQD
                     SPQRHGNLLRARWIAQRHPQSADPEPSATAPASVEAVPPGDPDADK"
     gene            2139..3182
                     /locus_tag="VFV87_06915"
     CDS             2139..3182
                     /locus_tag="VFV87_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103524.1"
                     /translation="MAGRCARRAFTLVELLVVILIIGVLISLLLPAVQKARAAAARTS
                     CLNNLKQIGLAMHNHECTLSYLPYSKRSTKPQRSWAPDLLAYLEQGNIVSDANYDLQG
                     NWWRTTDEVTGAPIPNGYTVQKFVAVFICPSSPIQHRIENKIDGAAGDKIGACGDYFA
                     PEGVSIAINNELPPAMQLNFGTATVLPGVLRPYEDSLPNIPPGSTYPYAFRKTKSDSV
                     TDGTSNTILIGECAGREDVWRGRVMRPAVADKSLPNCARARGGAWATNDNPYVIGTRV
                     DWCDSSTPIPGKMKINASNEHGFLYYSFHDGGANFCFADGSVRFISDQVALSVLAALT
                     TRSGGEALSSTDY"
     gene            complement(3696..4094)
                     /locus_tag="VFV87_06920"
     CDS             complement(3696..4094)
                     /locus_tag="VFV87_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103525.1"
                     /translation="MGNRPQNGARLSITKRCHHVKAPSILVFAPNLQQFALADEPRRQ
                     GQLKHPTRLAALQIGYLNSHLVASRPNPILNRGEVHDLPAAKLCSLLVVVTDALDGRT
                     PIPRQALAGQGSRVSVHRVCGLPMEDSADE"
     gene            4212..4937
                     /locus_tag="VFV87_06925"
     CDS             4212..4937
                     /locus_tag="VFV87_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103526.1"
                     /translation="MAKVIVERPRGGGGVRRPKGCRKRERLAQADLLPSREGYRRRWV
                     NASYQKHLNEHLSPLRRYLASQVGRPWNKVHSEISRHLRLDSAVQSHVLDHLDDFVER
                     HVLVVDGEFLSSSGWNAGRPLRSLFYVCPRTGLLRRNKRPWRRHRQAPQPPDFVTIDN
                     RRQLHRIDGLWYEITLVAIAPGEQRFDVLLKSVVGGSVFGSAQPTCRMALVATAKRQL
                     NKREIRRAEQLLEEQRRTFCPRK"
     gene            5160..>5405
                     /locus_tag="VFV87_06930"
     CDS             5160..>5405
                     /locus_tag="VFV87_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5103527.1"
                     /translation="MPAALQRPTLVLNRNWQPIRVSTVARSLILVWNDHARIVDPHDF
                     QTYDWADWTAMAPSDDEVFIQAVSYRLRVPEVVALTRY"
BASE COUNT         1023 a         1745 c         1629 g         1008 t
ORIGIN      
        1 tggcgggctg ttcgaccagg ccggccatta cgccggtcaa aaagacggaa gccgagcagc
       61 ttgccaatcg agacgatttg agcctggcgc tcgacagcct gcgtaagctg gcggaaggtc
      121 ccgatccgca agccgaacag cggacgattt tctatctgaa tcaatggctg gcgtccacca
      181 agccttcagc cggcgagtgg aaaccggatc ggctgctcac gaatctgcca cggtccctta
      241 attatacgct tggcatggat cggctgagcg accttcattt ttcctacacg atcaaattgc
      301 cctctgcgct ggtcatcgcg atgggaggtg atcccgagaa ctatgaagtt cctgagattc
      361 ccggcaccga cttacctcat ctgcaaactt ccctctggat gcatgatatc gcccaccgcg
      421 caaagcaaac cacgataccc gacgagttag cgcagtggtt gacggcgcac gagaagcaga
      481 tcggtatcgc cgaggccgag caacttgcct ccgccgagcg ggttttcgat tggacgatcc
      541 gcaacatcca gctcgacgaa ctgcccccgc cgcccaaggg tcccgcagcc acggccgacg
      601 ccaggacaga gcccgtggcg cccgccctgt tgggcgagcc tgggccaggc tatcggcagt
      661 tgccacggga tgtgctggtc cacggccacg gcgacgcctg ggagcgggct cgcgtcttca
      721 ttctcctttg ccggcagctg cagatcgaca cggtcatgct cggcctcatt caagaccaat
      781 cccccgtccc ccgcggctgg cttcccgcgg ttctgatcaa agacgagctg tacttattcg
      841 acacgtcgct gggcctgccg attcccgggc ccgaaggcaa gggcattgcc accctgagcc
      901 aggtgctcgc cgatccgcgg cttttacgga agcttgacgt cgagggagag ccgccctatc
      961 ccgtcaccga gaaggacttg aagatcgccg ccttgatcga agccgagccc gacgcgcttt
     1021 cgcaccgaat gcaattgctc caaggcgcat tgccggccga tcagcacctg attctcaccg
     1081 cgcggccaag cctgctcgaa cccaagctgc ggaagtgccg ggggatcggc cagatcagct
     1141 tgtggcaggt tccgttcgag gcggccatgt atgcctttcc cttgggccgg gcacggggcg
     1201 ccgctcagga tccgctggcg gcggcacaat tgcagctcga gttggagaca tttaatccca
     1261 atttgccgct agccaaggga cgcaacctgc acttgcaagg tcggttcgaa aagcaggacc
     1321 aggcgctcgg cgcccgcaat cactatttgg cctcccgtcc tcccgatcag gaaatgcagc
     1381 gggtcgaaag ctcggaaatc gttcgcaagg cgatgggatt ccagcaaagc ttgcccgcgg
     1441 gtgaaaagga gcaaaagaag gaaatcgaat tcagaatgac gctggcccgc acgcggaagc
     1501 atcacgccac ctactggatg ggcctgactt atcaggaagc cggcaatcaa tcggccgcca
     1561 tcgaatggct ggcggaccgg acgatggaag cctatccgcc cagcccatgg attcccggcg
     1621 cacgctacaa cttggcgcgc tgctatgaag atctaggcca atgggaggcc gcccgcaaat
     1681 ggctgctctc cgatcaggat tccccccagc ggcacggcaa tttgctccgt gccagatgga
     1741 tcgcccaacg gcatccgcaa agcgctgatc ccgaaccgag tgcgaccgca cccgcatcgg
     1801 ttgaggccgt tccgccaggc gaccccgatg ccgacaagta gtcgccgcct ctcattcgat
     1861 tgttcaccat ggcgatttgc tcgacacgca tgtcgagttt tctgacgatc aacgccgcgt
     1921 ggaagcctcc ttgcggatgc ggtcacggca attgctcccg agattcggcg gctaagcgtt
     1981 ccatctccgg cttaatgcat ggaaacggca cgcagcgtgc attgcgtcgg cggcactctt
     2041 gattcgtact cgcgtgcgct tgccgacggc tcgccgtcgg gcggctgcgc acgcggccga
     2101 tcctcgtttt acctgcccac cggaaaggaa cctgtcgtat ggctggtcgt tgcgcacgcc
     2161 gcgccttcac attggtagaa ctgctggtcg tcattttgat tattggcgtc ctgatctcct
     2221 tgctgttgcc agcggtgcaa aaggcccggg cggcggccgc acgcacgagc tgcttgaaca
     2281 acctgaagca gatcgggttg gcgatgcaca atcacgaatg cactctgagc tatctcccct
     2341 attcgaaacg atctaccaag ccgcaacgta gttgggcgcc cgacctgctg gcgtatttgg
     2401 aacagggcaa catcgtgagc gacgccaact acgacctcca aggcaactgg tggcgaacga
     2461 ccgacgaagt gactggcgct ccaattccca acggctatac cgtccaaaaa ttcgtcgccg
     2521 tcttcatttg cccatcgtcg cccatccagc atcgaatcga aaacaaaatc gacggagccg
     2581 ccggcgacaa gatcggcgcg tgcggcgatt atttcgcgcc ggaaggcgtt tccatcgcga
     2641 tcaataatga attgccaccc gcgatgcagc tcaatttcgg aactgccacc gtcttgccgg
     2701 gtgtgctgag gccctatgag gacagtttgc cgaacattcc tcccggcagc acgtatccct
     2761 acgccttccg caaaacgaag tccgactccg tcacggacgg cacatccaac acgatcctga
     2821 tcggcgagtg cgcgggccgc gaagacgtct ggcgcggccg cgtcatgcgt ccggctgtgg
     2881 ctgacaaatc gctgcccaat tgcgcccgcg cccgtggtgg cgcgtgggcc acgaacgaca
     2941 atccgtatgt gatcggcacg cgcgtcgatt ggtgcgacag ttcgacgccc attcccggca
     3001 agatgaaaat caatgcctcg aacgagcacg gctttctcta ttacagcttc cacgacggtg
     3061 gggccaactt ttgttttgcc gacggttcgg tgcggtttat ttccgatcaa gttgccctct
     3121 cggtgttggc ggcgctaacc acgcgctccg gcggcgaggc gttgagctca accgattact
     3181 gacccaccgc gcccctatga ccaatggctt cggcacggca atcacaactc ggtgagtcga
     3241 gatcggccaa cgggactcct gcttcgccgc acgccgtcct gggcggcgag ctcgccgtcc
     3301 cgctggcctg cccgcgctgt ggagcgccag gcagcgtgcg gtgggagcat ttgggacatg
     3361 acctgcgctg ccccaagtgt cgggtctggt tccaggtcga gcgcaacggc cagagtgtgc
     3421 gctggcacga gaagcatccc tactcttgcc cccgctgtcg ccagcgcggc tcgctggttc
     3481 tcgccacgca gcaacccgcc tgccccggct gcgggctgcg gctcttggcc ggccccagcg
     3541 gccgccttta ttcggcccac gagctggaga agatcgaaga gcgctcccgc gaggcgggta
     3601 ggaaggcgca gtacgcaatc ccgccagcag gacacgccag tgcgcctcgg cgatttgtag
     3661 ttgtcttggc tggcgccaca cttggtgccc ttgtcttact cgtcggcgga gtcttccatt
     3721 ggcaatccac agacacggtg cacgctcacg cgcgaacctt gacccgccag tgcctggcgg
     3781 gggattggcg tgcggccatc gagagcgtcg gtgacgacga cgagcagcga gcagagtttc
     3841 gccgctggca aatcatgtac ttcgcctcgg ttgaggatcg gattcggccg gctggcgacg
     3901 aggtgcgaat tcaagtagcc gatttgcaag gcggcgaggc gagtcgggtg cttgagctgt
     3961 ccttgtcgtc gcggttcatc ggcgagcgca aactgctgca ggtttggcgc aaaaacaagg
     4021 atgcttggcg ctttgacgtg gtggcatcgc ttcgtgatcg acaagcgggc gccgttctgc
     4081 ggcctgtttc ccattgaccg cctcgaaacg gcgcgaacct cctttatctg cagcggctct
     4141 ctggagcaat gccgcactcc attgcgacat gagaaatccc tcaggagcac aatgctcata
     4201 tgcgcgccga catggccaag gtcatcgtgg agcgaccacg cggaggcggc ggagtccggc
     4261 ggcccaaggg ctgccgcaag cgcgagcgat tggcccaggc cgacctgttg cccagccgcg
     4321 agggctatcg ccgccgctgg gtgaacgcga gctatcagaa gcacctcaac gagcacctgt
     4381 ccccgctgcg acgatatctg gcctcgcagg tcggccggcc ctggaacaag gtccatagcg
     4441 agatcagccg ccatctgcgg ctcgattcgg ccgtgcagag ccacgtcctg gatcacctgg
     4501 acgacttcgt tgagcgtcac gtcctggtcg tagacggcga attcctcagc agcagcggct
     4561 ggaacgcggg ccggccgctg cggtcgctgt tctacgtttg cccgcgaacg ggtctcctgc
     4621 gaaggaacaa acggccctgg cggagacatc ggcaagcgcc gcagcccccc gatttcgtca
     4681 cgatcgataa tcggcggcaa ctccacagaa tcgacggcct ttggtacgaa atcactctgg
     4741 tggcaatcgc gcccggtgag cagcggttcg acgttctgct caagtcggtt gtcggcggct
     4801 ctgtctttgg cagtgcgcaa ccgacctgcc gcatggcact agtcgctact gccaagcgcc
     4861 agctcaacaa gcgcgagatc cgccgtgccg agcaactcct cgaagagcag cggcgaacgt
     4921 tctgcccccg caagtaagcc agccggcaga attccacaag cgaaccaccg ccacgcgatg
     4981 cgcctctttc gtcgacggcg gatgcttaag acttcgtgaa gaactcgcga gttgggcttt
     5041 cccgcgctac caccgcctga gtagagttta gtaagatgtt gtggggtcag gttttgcggc
     5101 cccacggctg ggctgctgag cgcagccgcc gagaccttcc atcaccattc gtgttcgtca
     5161 tgcccgcagc acttcagcga ccgacgcttg tcctcaaccg caattggcag ccgatccgcg
     5221 tttcgacggt ggcccggtcg ctgatcctgg tgtggaacga ccacgcgcgg atcgtcgatc
     5281 cgcacgactt ccagacgtat gactgggccg actggacggc gatggccccg tcggacgacg
     5341 aggtgttcat ccaggccgtc agctaccggc tccgcgtgcc ggaggtggtg gccctgaccc
     5401 gctac
//
LOCUS       DASUGB010000358         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1013927, whole genome shotgun sequence.
ACCESSION   DASUGB010000358 DASUGB010000000
VERSION     DASUGB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013927"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2591
                     /locus_tag="VFV87_06935"
     CDS             <1..2591
                     /locus_tag="VFV87_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5103528.1"
                     /translation="HSPAACHQRASGMSLRRYSWRAIQQRPGRTILTVLSIVIGVTAV
                     VGISLGAATTRNAYKQMFAVVTGRATLEIDALGGGGFDQAVFEKVAGIPGILAATPLV
                     DRPTSMSYGDDKRIRMEVLGIDPERDRSVRDYEITEGRQVKPGEDEIVLDAGFAKYLE
                     LKVGDEVRLLTKRLSKPFTIVGLLETKTGAAFTQSSIGLMGIDRARYHFNPRGRSELI
                     DKIQIVTATEIDPEVVAAQISPLLPDGVQVHQPAARTQLMSETLRSGESGLRTTTLFA
                     FLLAAFIILNTFLMNVSERRRHISIMRAIGATRQQMTRSIIEEGLWFGVIGTVIGMGL
                     GVAMAYVATYVLSVTFQVQLPRIAEVMTPWPFISGAVFGLLMAFLGSAVPAWLAGRVS
                     PLEGMNRIVRGKTRSFTLTLLIGGAMLTLGSLGVIFTCIYGYLPIEVAQFFAVPFLIG
                     VVMLDSVVLAPQAAFVAWWLRLFARVEATLSLKQVLRHHLRTVLTVGMLFIAGSTGVG
                     MANSILDNIRDIYDWYDQAIQGDFFVRAMMPDMERGDAADLPYALGDELKAIPDIRLS
                     SAAFVEARIHPSGAKDDEPLNAIAIARTLTDAQPAFDLVEGDSSTLRDKLLHGEVVIS
                     TVIAQKLKAQVGDTIALETKEGIQRIPICGIANEYMVGGMAVHMSFESGQKWLDIEGV
                     DGYVIRCKDEHQAAALRPQLAEICKKYDVFLLSRADIRQNVAGFVNGVEWLLWVLVMM
                     LFVVAAIGVVNTLTMNVLEQTRELGLLRIVAMTKRQVRRTILMQAVIIGCISLPSGVA
                     MGLAVAYVLNMAMPASIGRPIDFNFYPLLLFLTLGGAFVIVLVAAYFPARRATRINVV
                     EALHYE"
     gene            complement(2619..4013)
                     /locus_tag="VFV87_06940"
     CDS             complement(2619..4013)
                     /locus_tag="VFV87_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103529.1"
                     /translation="MSDAFSLSRTCRQFRAQTHRRGFLHAGVLGMAGLSLASLLRSEA
                     AAAKGAPARAAGDKRGNNVILLWMRGGPSHIDMWDPKPDAPAEVRGEFGTMNSAMPGI
                     QLTDMLPKTAAIMDKWSIVRSLTHNDAGHSTGDQMCFTGYGPGLQPDENIHPSCGSIV
                     SEQLGRLNPELPAYVMIPRMVPGTGSAYLGVSHKPFETGADPAQSGPFKLPNFSLADG
                     LSVDRLGSRRDLLTDLDRLRAQVDASGKMEGLDQFGQKAWEMLSSPVAQKAFDLDAEP
                     QALRERYGFMPAFDPGAADRCGAPAWSQRILLARRLVEAGVRLVTVDLRWWDTHVLGF
                     DSLRRGFLPRWDQAYSALVEDLTQRGLLERTLVVAWGEFGRTPRVNNNAGRDHYPNVF
                     SAAICGGKVKGGRVVGSSDSKGAFPKDNPKPPQDVLATIYDHLGIDVSKHYLNDAGRP
                     IRTLPFGEPIRELF"
BASE COUNT          747 a         1350 c         1350 g          804 t
ORIGIN      
        1 cccattcgcc cgcggcgtgt caccagcgag catccggcat gagtctccgc cgctacagtt
       61 ggcgagccat tcagcaacgc cccggccgca cgatcctgac ggtgctgagc atcgtcatcg
      121 gcgtcaccgc ggtcgtgggc atcagcctgg gcgctgcgac gacccgcaat gcctacaagc
      181 agatgtttgc cgtcgtcacc ggccgggcga ccctcgaaat cgacgcgctg gggggcggcg
      241 ggtttgacca ggcagttttc gaaaaagtcg ccggcatccc gggcattctg gcggctacgc
      301 ctctggttga ccgtccaacg tcgatgtcgt acggcgacga taagcgcatt cgcatggaag
      361 tgctgggcat agatcccgag cgcgaccgca gcgttcgcga ctacgagatt accgaaggcc
      421 gtcaggtgaa gccgggcgaa gacgaaatcg tcctcgatgc gggctttgcg aagtacctgg
      481 agctgaaggt gggcgatgaa gttcgcctgc tcaccaaaag gctctccaag ccgtttacga
      541 ttgtcggcct gctcgaaacg aagaccggcg cggctttcac gcagtcgtcg atcgggctga
      601 tgggcatcga ccgtgcccgc taccacttca acccgcgcgg ccggtcggaa ctgatcgaca
      661 agattcagat cgtcaccgca acggagattg accccgaagt cgtagcggca cagatttcgc
      721 cgctgcttcc ggacggggtc caggtccacc agccggcagc acgcacgcag ctcatgagcg
      781 agacgcttcg ctcgggcgag agcggcctgc gcacgaccac gctgttcgct tttctcctgg
      841 cagctttcat tatcctcaac acgttcctga tgaacgtcag cgagcggcgg cggcacattt
      901 cgatcatgcg ggccatcggc gccacgcgcc agcagatgac ccgttcgatc atcgaagagg
      961 gcctctggtt cggggtcatc ggcaccgtga tcggcatggg gctcggcgtg gcaatggcgt
     1021 acgtcgccac ctacgtgctc agtgtgacct ttcaggtgca gttgccgcga atcgccgagg
     1081 tgatgacccc gtggccgttc atctcgggag cggtgtttgg cctgctcatg gcgtttctcg
     1141 gatccgccgt gcccgcctgg ctggccgggc gggtctcgcc gctggaaggc atgaaccgta
     1201 tcgtccgcgg caagacgcgc agctttacgc tcaccctgct gatcggcggg gcaatgctga
     1261 cgctgggcag cctgggcgtc atctttactt gcatttatgg ctacctgccc atcgaagtgg
     1321 cccagttttt tgccgtgccg ttcctgattg gcgtcgtgat gctcgatagc gtggttctcg
     1381 ccccgcaggc tgcgttcgtt gcctggtggt tgagactttt tgcccgtgtc gaagccaccc
     1441 tcagcctcaa gcaggttttg cggcatcacc tgcggaccgt cctgaccgtc ggcatgttgt
     1501 tcatcgcggg cagcacggga gtgggaatgg caaactcgat actggacaat attcgcgaca
     1561 tctacgattg gtacgatcag gcgatccagg gcgatttctt cgtgcgcgcg atgatgcctg
     1621 atatggaacg gggcgacgcc gccgatttgc cgtacgcctt gggcgatgaa ttgaaggcga
     1681 tcccggacat tcggctctcc agtgcggcct ttgtcgaggc ccgcatccat ccatccggcg
     1741 ccaaagacga tgagccgcta aacgcaatcg ccatcgctcg cactttgaca gacgcgcaac
     1801 cagcctttga tctcgtggaa ggcgactcct ccacactgcg cgataagctg ctccacggcg
     1861 aggtcgtcat cagcaccgtc atcgctcaga aactcaaagc gcaggttggc gacaccatcg
     1921 ctcttgaaac caaggaaggc atccagcgga tccccatttg cggcatcgcc aacgagtaca
     1981 tggtgggcgg aatggccgtc cacatgagct tcgaatcggg gcagaagtgg ctcgacatcg
     2041 aaggagtcga tggctacgtc attcgttgca aggacgagca tcaggccgca gcacttcggc
     2101 cccagctcgc ggagatctgc aagaagtacg acgtgttcct cctctcgcgg gccgacattc
     2161 gccagaacgt cgccggattc gtcaacgggg tggaatggct gctgtgggtc ctcgtaatga
     2221 tgctcttcgt cgtggcggcc attggcgtcg tgaacaccct gacgatgaac gtcctcgagc
     2281 aaacccgcga gctcggcctc ctgcggatcg tcgccatgac caaacggcag gtccggagga
     2341 cgatcctgat gcaggccgtc atcatcggct gcatcagcct gccttctggc gtggccatgg
     2401 ggctcgcggt cgcttatgtg ctcaacatgg ccatgcccgc gtccattggg cggccgatcg
     2461 acttcaactt ctatccgctg ctgttgttcc tgacgctcgg cggcgcgttt gtgatcgtcc
     2521 tggtcgccgc ctatttcccc gcccgccgcg ccacgcgcat caatgtcgtt gaggcgctgc
     2581 actacgaata attgccggcg aataattgcc ggcggcccct agaacagctc gcggatcggc
     2641 tcgccgaagg ggagtgtgcg aatcgggcgg ccggcgtcgt tgagatagtg cttgctcacg
     2701 tcgatgccga gatggtcgta gatcgtcgcc aggacgtctt gcggcggctt gggattgtct
     2761 ttggggaagg cgcctttgct gtcggatgag ccaacgactc ggccgccctt cactttgccg
     2821 ccgcagatcg ccgcgctgaa cacgttcgga tagtgatcgc ggccggcgtt gttgttcacc
     2881 cgcggcgtgc gaccgaactc gccccaggcc acgaccagcg ttcgctccag caggccccgc
     2941 tgagtcaggt cctcgaccag ggccgaatac gcctggtccc accgcggcag aaagccccgc
     3001 cgcagcgaat cgaagcccag cacgtgcgtg tcccaccacc gcagatcgac cgtcaccagc
     3061 cgcacccccg cctcgaccag ccgcctggcg agcagaatcc gctggctcca ggcgggtgcg
     3121 ccgcagcggt cggctgcgcc gggatcgaac gccggcatga agccgtaacg ctcgcggaga
     3181 gcctgcggct cggcgtccag atcgaacgcc ttctgggcga ctggcgagct gagcatctcc
     3241 caggccttct ggccgaactg gtccagccct tccatcttgc cgctggcatc gacctgggcc
     3301 cgcagccggt cgagatcggt cagcaagtcg cgccgcgagc cgagccgatc gaccgagaga
     3361 ccgtcggcca gcgaaaaatt cggcagcttg aaaggaccgc tctgtgccgg gtcggccccg
     3421 gtctcgaagg gcttgtgact gactcccagg tacgccgacc cagtgccggg gaccatccgc
     3481 gggatcatca catacgcggg caattccgga ttcagccgcc caagctgctc gctgacgatc
     3541 gagccgcagc tggggtggat attctcatcg ggctgcaatc cggggccata accggtgaaa
     3601 cacatctggt cgccggtgga gtggccggcg tcgttgtgcg tgaggctgcg gacgatcgac
     3661 cacttgtcca tgatggccgc ggtcttgggc agcatgtcgg tgagctggat tcccggcatg
     3721 gcggagttca tcgtgccgaa ttcgccgcgg acctcggccg gcgcgtcggg cttgggatcc
     3781 cacatgtcga tgtggctggg tccgccgcgc atccagagga gaatgacgtt gttaccgcgc
     3841 ttgtcgcctg ccgctcgcgc cggtgcaccc ttcgccgccg ccgcttcgga cctgagcaaa
     3901 gaagccagcg acaaaccggc catgcccagc acccccgcgt gtaagaagcc gcggcggtgt
     3961 gtttgggcgc gaaactgccg gcaggtgcgg gagaggctga aggcgtcgct catgaggccg
     4021 aactgggtgg gatttcaaag gcgggcaaac gggccgtcgg acatacccat ctaattcagt
     4081 ttatacttgc gcgccctcgc ttaaagcaag caataagctg caggaacgtc ttctgcagca
     4141 gttgtcggag cgagaatatc tgcgaccacg ggcggctaag cggcggatcg tcccgcaaga
     4201 atggccgggc tgggcgacga gacctccgcg gcgagttgcc ggtagtcttc g
//
LOCUS       DASUGB010000359         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_735222, whole genome shotgun sequence.
ACCESSION   DASUGB010000359 DASUGB010000000
VERSION     DASUGB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735222"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFV87_06945"
     CDS             complement(<1..1310)
                     /locus_tag="VFV87_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5103530.1"
                     /translation="MKSASKQVTWIIALAAVGLAHDASAVDPAELEAAGDKLFARDNL
                     MAWCIVPFDAKKRGPEERAEMLAKLGFKHFAYDWRAEHIPTFDAEIAALKKHGVALDA
                     WWFPGQLNAEAKQILDALKRNGVRTQLWVTGGGGPTKSPEEQQQRIAAEVARLKPICE
                     AAKEIDCTVGLYNHGGWFGEPENQLEVLKALDMPNVGLVYNQHHGHDHIDRFEALLQR
                     MKPHLLCLNLNGMTYDGEKKGTKILPLGTGELDLPLLQVIAKSGYTGPIGILNHTDLD
                     AEARLRDNLDGLAWLVKRLDGSNAGPRPKYRTYDVDSAAKPGGTAKAAGYANAALGGG
                     LVAAGRDEYRNAPITVECRATLTQKARYNILVACDTKASAAHWENFSMAGDGTFTAFL
                     PGRDPDHVRSSVNIADGQPHDLAMILEKDRVRLFVDGNQVADQAV"
     gene            complement(1565..3331)
                     /gene="ptsP"
                     /locus_tag="VFV87_06950"
     CDS             complement(1565..3331)
                     /gene="ptsP"
                     /locus_tag="VFV87_06950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX5103531.1"
                     /translation="MRKGIPVSPGVAVGTAYCIHEIFVNPDTQRLEDHEITAELANYE
                     TARDKAAAELRALQHKVEQQVGHDEAAIFGVHESILRDAAFTSKVRGWIVNDRLTSQA
                     ALGRLLDEYTVVFTRTNDEYLKERLNDLRDVVVRVVAHLSVQKPVDDGNLLEGPVIVV
                     ADELLPSQAVALGGVNVRGIVTQAGGQTSHAAIIARSRGIPAVSGVRGVLRQVKTGDT
                     LVVDGRSGGVLINPDSEQRMAFLKLEREFFHLKDQLAANRDQPARTADGITLELLANV
                     NSPTDAKAAVAMGASGVGLFRTEYIYLTHPDVPDEEEQLAAYRDVIEASPGRLVTIRT
                     LDIGGDKTVPYLGHTHQEANPFMGWRSIRLSFEHPEFFSTQLRAILRAAAEAHAKGGR
                     VRLMFPMITALEEIRKVRAMVRRATQQLEAEGKAFVELPIGMMLEVPAAAISIADLLP
                     HVDFVSVGSNDLVQYLMAADRDNPKVSHLCQPLAPPVLRVLATVIKACQKANKPITLC
                     GEMAGQPRPFVLLFAMGLRSFSMSPAFIPSMKELAAHLTEESARQVLRKALTLRTTAA
                     VKRFMAEQLAKIAPNLRLMDTD"
BASE COUNT          625 a         1118 c         1159 g          711 t
ORIGIN      
        1 accgcctgat cggcgacttg attgccgtcg acaaagagcc gcactcggtc tttttcgagg
       61 atcatcgcca gatcgtgcgg ctggccgtcg gcgatgttga cgctgctgcg gacgtggtcg
      121 ggatcacggc cgggaagaaa agcagtgaat gtgccgtcgc cggccatcga gaagttttcc
      181 cagtgggcgg ctgaggcctt ggtgtcgcag gcgacgagga tgttgtagcg ggctttctgc
      241 gtcagcgttg cgcggcattc gaccgtgatc ggggcattgc gatattcatc gcgccccgct
      301 gcgaccaagc cgccgccgag tgcggcgttc gcatagccgg ccgctttcgc cgtgccgcct
      361 ggtttggccg cggaatccac gtcataggtg cgatactttg gccgcgggcc ggcgttgctg
      421 ccgtcgagtc gcttcaccag ccacgccagc ccgtccaggt tgtcccgcag ccgggcttcg
      481 gcatcgagat cggtgtgatt gagaatgcca atcggcccgg tgtaaccgct cttggcgatc
      541 acctgcaaca gcggcagatc gagttcgccg gtgccaagtg gcaggatctt cgttcccttc
      601 ttctcgccgt cataggtcat gccgttgagg ttcaagcaca acaaatgcgg cttcatccgc
      661 tgaagcaggg cctcgaagcg gtcgatgtgg tcgtggccgt ggtgctggtt gtaaaccaga
      721 cccacgttcg gcatgtccaa ggccttgagg acttcaagct ggttctccgg ctcgccgaac
      781 cagccgccgt ggttgtagag gccgaccgtg cagtcgattt ccttggccgc ttcgcagatc
      841 ggcttcaagc gggcaacttc tgccgcaatt cgctgctgtt gttcttcggg cgactttgtc
      901 ggcccgccgc cgccggtgac ccagagctgc gtgcggacac cgttccgctt cagcgcgtcc
      961 agaatctgct tcgcctcggc gttcaattgg cctggaaacc accacgcatc gagagccacg
     1021 ccgtgcttct tcagcgcggc gatttcggca tcgaaggtcg gaatgtgctc ggcccgccaa
     1081 tcgtaggcaa agtgcttgaa gccgagcttc gcaagcattt ctgcccgctc ttccggcccg
     1141 cgtttcttgg catcgaacgg aacgatgcac caggccatca ggttgtcccg cgcaaagagc
     1201 ttgtcgccgg cagcctccag ttccgcggga tccaccgcac tggcatcgtg agcaagtccg
     1261 actgccgcaa gtgcgatgat ccacgtgact tgtttggatg cagatttcat ggagtggata
     1321 ttacgacgct tgatccgtcg gtcgcaagtt gggccagtcg tccgctggcc attaaacgca
     1381 aggacgcaaa ggagcgcaga ggcgcaagga gggaaggaag gaaagaggaa gtgaatcgtc
     1441 cttctttgcg cctctgcgct ccttcgcggc tttgcgttgc cgccgaaacg ctcgccccac
     1501 gcggacgccc tcgcttgcgc gtcgggttgg tgtggcgcgg gccggcgaga cgtctgcccc
     1561 gcggctaatc cgtgtccatc agccgcaggt tgggagcgat tttcgcgagc tgctcggcca
     1621 tgaagcgctt gacggcggcc gtggtgcgga gcgtaagggc cttgcgaagg acctggcggg
     1681 ccgattcttc ggtcaggtgc gctgccagct ccttcatcga cggaataaac gccgggctca
     1741 tgctgaagct ccgcagaccc atcgcaaaca gcagcacgaa cggccgcggc tggccggcca
     1801 tctcgccaca gagggtaatc ggcttgttgg ccttctggca ggctttgatg acggtcgcca
     1861 gtacacgcaa aaccggcggc gcgagcggct ggcagagatg gctcactttg ggattgtcgc
     1921 ggtcggcggc catcaaatac tgcaccaggt cgttggagcc gacggacacg aaatccacat
     1981 gcggcaacag gtcggcgatg gagatggcgg ccgccggcac ttcgagcatc atgccgatcg
     2041 gcaactcgac aaacgccttc ccctcagctt cgagctgctg ggtcgcgcgg cggaccatcg
     2101 cccgcacttt gcgaatctcc tccagggccg taatcatcgg aaacatcagc cggacgcggc
     2161 cgcctttcgc gtgtgcttct gccgcggccc ggaggatcgc gcgaagctgc gtgctgaaga
     2221 actcgggatg ctcgaacgag aggcgtatac tccgccagcc catgaacggg ttggcttcct
     2281 ggtgtgtgtg gcccaggtac ggcaccgttt tgtcgccgcc gatgtcgagc gtgcggatcg
     2341 tcaccaggcg gcctggactg gcctcaatca catctcgata tgcggcgagc tgctcctcct
     2401 catctggcac gtccgggtgg gtgaggtaaa tgtattcggt tcgaaagagg cctacgccgc
     2461 tggcccccat ggcgacggct gctttggcgt cggtggggct gttcacgttt gccagcagct
     2521 ccagggtgat gccgtcggcc gtgcgggcag gctgatcgcg gttggcagca agctggtcct
     2581 tgaggtggaa gaactcgcgc tccagcttga ggaacgccat ccgctgctcg gagtcggggt
     2641 tgatgagcac gccgccgctg cgcccatcga ccaccagcgt gtcgcccgtt ttcacctgac
     2701 gcagcacacc ccgcacgccc gaaacggccg gaatgccgcg gctgcgagcg atgatcgcgg
     2761 catggctcgt ttggccgccg gcctgtgtaa cgattccacg aacattgacg ccccccaggg
     2821 ccaccgcttg cgatgggagc agctcgtcgg caacgacaat caccggccct tccaacaggt
     2881 tgccgtcatc caccggcttt tggactgaaa gatgcgcgac gacgcgcacg acgacgtccc
     2941 gcaggtcgtt cagccgttcc ttcaggtatt cgtcgttggt gcgggtaaag acgaccgtgt
     3001 actcatccaa aagtcgtccg agagccgcct gcgaggtgag ccggtcgttg acgatccacc
     3061 cgcggacttt gcttgtgaag gcagcatcgc ggaggatcga ctcgtggacg ccaaagatgg
     3121 ccgcctcatc gtgcccaacc tgttgctcca ccttgtgttg cagcgcgcga agctccgctg
     3181 ccgccttgtc gcgggcggtt tcgtaattgg ccagttcggc agtgatttcg tggtcttcca
     3241 gccgttgcgt atcgggattc acgaatattt cgtgaatgca gtaagccgtg ccgacagcca
     3301 cgccgggaga gacgggaatt cctttgcgca tcgacgggag aatgtaccac cgggcgcgtc
     3361 cggtgacaaa cgcccgggac agccggaacg ttataacgtc ctgtccgcca gcaggttgtg
     3421 gcggacggaa gagaaacgga ctggttgaca ggcgcggctg gttgtacccg cgaagtgtaa
     3481 gcgatcagtc cccagagcca gacagcatat ccagcaacga tggctccaac tcggctttac
     3541 ctcccaggtt cagccattcc aggtcctggc cgtcgtcggc ggcagccagg accagatcga
     3601 cgcccgccgg ggt
//
LOCUS       DASUGB010000360         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_457740, whole genome shotgun sequence.
ACCESSION   DASUGB010000360 DASUGB010000000
VERSION     DASUGB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457740"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFV87_06955"
     CDS             <1..704
                     /locus_tag="VFV87_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103532.1"
                     /translation="PASGLLTTPIWKYIRLEPATGSHIALAFDSGDPAIVEAQFGRGR
                     CVLVATAASPDSIDRTAEPPTPWTALSSWPSFPPLVHEMLQFAVAGQSEGRNLAVGDE
                     LTGIIAAGGLDQPVTLSGPDGLSERLPVVADGLGARWTFGGTATSGLYEAQADQTVQR
                     FAVNVNPRESDLARFDPELLPSQIHREPLGVNEPATITAARDSSSYFRYVLFAVLGLL
                     LLEPVLAWQFGRGRG"
     gene            701..>3036
                     /locus_tag="VFV87_06960"
     CDS             701..>3036
                     /locus_tag="VFV87_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5103533.1"
                     /translation="MNGVWRDIERWLIGENLSADPGGLETSVQAASPVPPWLVLLLIL
                     ATAGVMLLVYWNESQTAGRVWKVLLAGMRTALVGLVVLMLYGWTVQRHRTDLPDVVVI
                     LDDSASMGLIDQYDDASLATEIKARLDKLNLPEATRLNLARTLLLENDAALITQLSQR
                     YNLRFYLAAISARAIGGERSETLAAIENATADQPASRLGNCLRDVLEAQRGRPTAAVI
                     MLTDGITTEGKSLVEAAQYARRKTVPLFLVGLGSDRPARDLRLADLLVEEAVFVGDLI
                     NFDVKLVHEGFTGDAIVKLTREGDSAVLDERKVKLGKPGVPQAIRLTHRADKAGEYEY
                     IVEVAPREGEANTDNNVLLRKVIVREETIRVLLVQAYPSFEFRFLKQMLLRELNANQP
                     ADAKAAGFRTVLQEADLEYVETDKTAERVFPVSRDELFTYDVLIFGDVNPALLSPSIM
                     QNIYDFVTVKGGGVIFIAGPRYTPLAFRDTPLAPLLPMNLDTVSVPDQDRVITEEFRP
                     RMTPLGRASPMLQLADDPAANEKLWQSGLAPIRWFASVPDLRPGVRVLAEHPTARTSD
                     NQPLPIIALQFVGAGKVVFHATDETHRWRFRVGDVYFARYWIQTIRYLCRSRLLSGNR
                     TAELTTDREQYGRGDVVQLRVRFLDDRLAPAADDGVTVVVQREGSERRSITLSRTTAD
                     RGVFEGSAAQLPEGQYRAWVAAPTLEGQPPSRQFSIVAPPGELARTQMDATELREVAK
                     VSVGKFYRFQDADKLLDDLPRGRQVRIESLPPRPIWNAP"
BASE COUNT          509 a         1032 c          990 g          505 t
ORIGIN      
        1 acccggcaag cggcctgctt accacaccca tctggaaata catccgcctg gagccggcga
       61 ccggctcgca catcgcgctc gccttcgata gcggcgatcc ggcgattgtc gaggcccagt
      121 tcggccgcgg acgttgcgtt ctggtcgcca ctgccgcttc gcccgattcg atcgaccgca
      181 ccgcggagcc accgacgcct tggaccgcgc tttcgtcgtg gcccagcttc ccgcccctcg
      241 tgcacgagat gctgcagttt gccgttgcgg gacagagcga gggtcgcaac ctggcggtcg
      301 gcgatgagct gaccggcatc atcgccgccg gtggactcga tcagccggtc acgctcagcg
      361 gacccgacgg cctgagcgaa cggctgccgg tggtggccga cggcctgggg gctcgctgga
      421 cgttcggcgg cactgccacg agcggcctgt acgaggccca ggcggaccaa accgtgcagc
      481 gattcgccgt gaacgtcaac ccgcgcgaga gcgacctcgc ccgcttcgac cccgaactgt
      541 tgcccagcca gattcaccgc gagccgctcg gcgtcaatga acctgcgacc atcacggccg
      601 cccgcgactc gtcatcctac ttccgctacg tcctcttcgc cgtcctgggc ctgctcctcc
      661 tggagccggt gctggcctgg cagtttggga ggggacgggg atgaacggcg tgtggcgcga
      721 tatcgaacgc tggctcattg gcgagaatct ctccgccgat cccggcgggc tggaaacgag
      781 cgtgcaagcc gcgtcgccgg tcccgccgtg gctggtgctg ctgctgatac tggcgacggc
      841 gggggtgatg ctgctggtgt attggaacga gagccagacc gcgggccggg tgtggaaggt
      901 gctgctggcg ggcatgcgga ccgccctcgt cggcctggtc gtgctgatgc tctatggctg
      961 gaccgtgcag cggcaccgga ccgatctgcc cgatgtggtc gtcatcctcg acgactccgc
     1021 cagcatgggc ctgatcgatc aatatgacga tgcctcgctg gcgaccgaga tcaaagcgcg
     1081 gctcgacaag ctcaacctgc ccgaagcgac ccgcctgaat ctggcccgca cgctgctgct
     1141 ggagaatgat gccgcgctga tcacccagct cagccagcgc tacaacctgc ggttctacct
     1201 ggccgccatt tcggccaggg cgatcggcgg cgagcgatcc gaaacgctgg ccgcgatcga
     1261 aaacgccacc gccgaccagc ccgccagccg gctcggcaac tgcctgcggg acgtgctgga
     1321 agcccaacgt ggccggccga ccgccgcggt gatcatgctc accgacggca tcacgaccga
     1381 aggcaagtcg ctggtcgaag cggcccagta tgctcgccgc aagacagttc ccctgtttct
     1441 ggtcggcctg gggagcgacc gcccggcccg cgacctgcgt ctggccgacc tgctcgtcga
     1501 agaagcggtc ttcgtcggcg atctgatcaa tttcgacgtc aagctcgtcc acgaaggctt
     1561 cacgggcgat gcgatcgtga agctgactcg tgaaggcgac agcgccgtgc tcgacgagcg
     1621 caaggtcaag ctcggcaagc ccggtgtgcc gcaggctatt cggctgacgc accgggccga
     1681 caaagcgggc gaatatgagt acatcgtgga ggtcgcgccg cgcgaaggcg aggcgaacac
     1741 cgataacaac gtcctgttgc ggaaagttat cgtccgcgaa gagacgatcc gcgtcctcct
     1801 ggtgcaggca tatcccagct tcgaattccg cttcctcaag cagatgctcc tccgcgagct
     1861 gaacgccaac cagcccgccg acgccaaagc cgccggcttt cgcacggtgt tgcaggaggc
     1921 ggacctggag tacgtcgaga ccgataagac ggctgagcgg gtgttccccg tcagccgcga
     1981 tgagctgttc acctacgacg tgctgatttt cggcgacgtg aatccggcgc tcctcagccc
     2041 gtcgatcatg cagaacattt acgacttcgt gacggtcaag gggggcggcg taatcttcat
     2101 cgctggcccg cgctatacgc cgctggcgtt ccgcgatacg ccgctggcgc cgctgttgcc
     2161 gatgaacctc gacacggtca gcgtgcccga tcaggaccgc gtgatcacgg aagaattccg
     2221 gccgcgaatg acaccgctgg ggcgggccag cccaatgctg caattggccg acgatccggc
     2281 tgccaacgag aagctgtggc agtcgggcct ggcgccgatt cgctggttcg cgagtgtgcc
     2341 cgatctgcgg cccggcgtcc gcgtgctggc cgagcatccg accgcccgca ccagcgataa
     2401 ccaaccgcta ccgatcatcg ccctgcagtt cgtcggcgca ggcaaggtgg tctttcacgc
     2461 gaccgatgag acgcaccgct ggcggtttcg cgtcggcgat gtctatttcg cccgctattg
     2521 gattcagacg attcgctatc tctgtcgctc gcggctgcta tctggcaatc gcacagccga
     2581 gctgacgacc gaccgcgagc aatacggccg cggcgacgtg gtgcagcttc gcgtgcgatt
     2641 tctcgacgac cgcctggccc cggctgccga cgacggcgtg acggtcgtcg tgcagcgcga
     2701 agggagcgaa cggcggagta tcacgctctc gcggactacg gccgaccggg gggtgttcga
     2761 aggctcggca gcgcagcttc ccgaagggca atatcgcgcc tgggtcgccg cgccgacgct
     2821 cgaaggccag ccgccgtcgc ggcagttctc gatcgtagcc ccgccgggcg aactggcccg
     2881 cacgcagatg gacgccaccg agctgcgcga ggtggccaag gtctccgtcg gcaaattcta
     2941 tcgatttcag gacgccgaca agctgctcga tgatctcccc cggggccggc aagtccgcat
     3001 cgaatcgctg ccgcccaggc ccatctggaa cgcgcc
//
LOCUS       DASUGB010000361         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1570048, whole genome shotgun sequence.
ACCESSION   DASUGB010000361 DASUGB010000000
VERSION     DASUGB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570048"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..400
                     /locus_tag="VFV87_06965"
     CDS             68..400
                     /locus_tag="VFV87_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103534.1"
                     /translation="MLESEAGHSPEVNPYASPHEARAQAAPPQTLVGKVLAIAGVLVS
                     LLFLSNLTFGGIIPLEIPDALPVIGNLDEVFFTGVLLASLSYLGVPLIPNFRAAGQAY
                     SRLEGPKR"
     gene            complement(546..1493)
                     /locus_tag="VFV87_06970"
     CDS             complement(546..1493)
                     /locus_tag="VFV87_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5103535.1"
                     /translation="MTTTNRRSFITASAVAIGAAATASSAVAQESNSAKIIEPPAGKQ
                     ILISCKLGMIAKKEGERELSLVERLKLAAAAGFDGVDFDEAGNFTPEQAQAAVQESGV
                     FVHNAINHAHWNQRLTSAKEQERAQGVKNIEHCLRVSHAAGGSGVLIVVGRGDDGPED
                     EINERARREIKRLIPLAASLGQPILFENVWNKMHYDHDAPPEQLPTRFIEFVDSFGSP
                     WVGMYYDIGNHWKYGQPLAWLRAFGRRAVKLDIKGFSRAKNKFVDITSADDDVPWKEV
                     QTGLAEIGFTGWTTAEVGGGGLERLTTVRQQMQKAFGLG"
     gene            complement(1630..2115)
                     /locus_tag="VFV87_06975"
     CDS             complement(1630..2115)
                     /locus_tag="VFV87_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103536.1"
                     /translation="MRIRKPVSQVSLEDLRKHPVWEFASDEEGNAGQDETTVRPVKFV
                     SPLELARGSFIVRSEFELRDGTKMLGYATPQAEDDSLAVLQPVIVTERGQVFFWHGVL
                     APGAKEIKESYKLLGRTAKETFPIELAIDVELSCTPPRATIPGFMVLVDWKTGETRVL
                     K"
     gene            complement(2178..>2652)
                     /locus_tag="VFV87_06980"
     CDS             complement(2178..>2652)
                     /locus_tag="VFV87_06980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652426.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5103537.1"
                     /translation="IQVEHPVTEMITGIDLIKTQIRVAAGQPLPFAQGDIVPRGAAIE
                     CRINAEDPDRGFRPSPGLIKQLYIPGGPGVRWDSHAHVGYTVPPYYDSMIGKLIVHRP
                     TREEAIACMIRALGELRIEGIKTTAPFHKRVLSHATFAEGLVDTKFVERTFLTEG"
BASE COUNT          485 a          842 c          796 g          529 t
ORIGIN      
        1 tttcaagcgt ccggcgcaac aagtcccaag attcccgcga ctgagccgaa ttacgaaggt
       61 gagtagcatg cttgaatctg aagctggaca ttcgcccgaa gtgaatccgt acgcgagccc
      121 tcacgaagcg cgggcccaag ctgcgccgcc gcagacgctt gtcggcaagg tcctggcgat
      181 cgcgggggtg ctggtttcgc tcctcttctt gtcgaatctc acgttcggcg gcattatccc
      241 gctcgaaatt cccgatgctc tgccagtgat cggcaacctg gacgaagtct tcttcaccgg
      301 cgtcctgctc gccagcctca gctacctcgg cgtcccgctc atccccaact tccgcgcagc
      361 cggccaggct tattcgcggc tggaaggccc gaagcggtga gacaccaacc cgacgcgcca
      421 gcgagggaac actaacccgc agcgtaagcg ggggaacact aacccgcagc gtaagcgagg
      481 gagtcctcga agccgacccg taggcgcagc actctctcgc ttacgcttcg ggttactatc
      541 ggcgactaac ccagcccgaa tgccttctgc atctgctggc ggacggtcgt gaggcgctcc
      601 aggccgccgc cgcccacttc ggcggtggtc cagccggtga agccgatttc ggcgaggccg
      661 gtttggactt ctttccaggg aacgtcgtcg tctgcggagg tgatatcgac gaacttgttc
      721 ttggcccggc tgaagccttt gatgtcgagc ttgacggccc gccggccgaa ggcccgcagc
      781 caggcgagcg gctggccgta tttccagtgg ttgccgatgt cgtaatacat gccgacccag
      841 gggctgccaa agctatcgac gaactcgatg aagcgggtcg gcaactgctc gggcggggcg
      901 tcgtggtcgt agtgcatttt gttccagacg ttctcgaaca agatcggctg gccgagcgac
      961 gcggcgagtg ggatcagcct cttgatctcg cggcgggctc gctcgttgat ctcgtcctcc
     1021 gggccatcgt cgccgcggcc gacgacgatg agcacgccac tgccgcccgc cgcgtgcgag
     1081 acgcgaaggc agtgctcgat attcttcacc ccctgcgccc gctcttgttc tttggcgctc
     1141 gtcagccgct ggttccagtg ggcatggttg atcgcgttat ggacgaacac gcccgattcc
     1201 tgcaccgcgg cctgggcctg ctccggcgtg aaattccccg cctcatcaaa gtccacccca
     1261 tcaaaccccg ccgccgccgc cagcttcagc cgctcgacca gcgacagctc cctctccccc
     1321 tccttcttcg cgatcatccc cagtttgcac gaaatcaaaa tctgcttccc cgccggcggc
     1381 tcgatgatct tggccgagtt cgactcttga gcgacagccg acgacgcggt cgcagccgct
     1441 ccgatggcaa cagcactggc ggtgatgaac gagcggcgat tggtcgtagt catgacaatc
     1501 ttcctttgga gtgcggcgac tcgtcgccgc ttttggattt cgattgaggt aagaagtacg
     1561 tgccgccatt gtgttccacg cggtgcgaca ggaaaagcaa ctaagccatg gaccgttaag
     1621 cggcgcaacc tacttcagaa cccgagtctc gcctgttttc caatccacta acaccataaa
     1681 tccggggatt gtagcgcgag gcggagtgca agagagctcc acatctatcg cgagttcaat
     1741 tgggaaggtt tccttggcgg tgcggcccag gagtttgtag ctctccttga tttcctttgc
     1801 tcctggcgcc aggactccat gccaaaagaa aacctgaccc cgttcggtga cgatcacggg
     1861 ctgcaaaact gcaaggctgt cgtcttctgc ttgtggcgtc gcataaccga gcattttggt
     1921 gccgtcgcgc aactcaaact ctgagcgaac aatgaacgat cctcgcgcca gctccagagg
     1981 actgacaaac ttgaccggcc tgaccgtcgt ttcgtcttga cccgcattcc cttcctcgtc
     2041 cgacgcaaac tcccaaactg gatgcttcct gaggtcttca agtgacactt gcgagacggg
     2101 cttccgaatt cgcatgagag aattccagcc aatccgaaag gaaaagcgat gacgagtcat
     2161 cgcactccaa atggaggtca gccctccgtc aaaaacgtcc gttcgacgaa cttcgtatcg
     2221 accaggcctt cggcgaaggt ggcgtggctg aggacgcgct tgtggaaggg ggcggtggtt
     2281 ttgatccctt cgatccgcag ctcgccgagg gcccggatca tgcaggcgat cgcttcttcg
     2341 cgcgtcgggc ggtggacgat cagcttgccg atcatcgagt cgtaataggg cggcaccgtg
     2401 tagccgacgt gggcgtgcga atcccaccgc acgccggggc cgccgggaat atagagctgc
     2461 ttgatcagcc cgggcgacgg ccggaagccg cggtcggggt cttccgcgtt gatgcggcac
     2521 tcgatcgcgg cgccgcgggg gacgatgtcc ccctgggcga acggcagcgg ctggccggcg
     2581 gcgacgcgaa tctgcgtctt gatcaggtcg atgccggtga tcatctcggt cacggggtgc
     2641 tcgacctgga tg
//
LOCUS       DASUGB010000362         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_875378, whole genome shotgun sequence.
ACCESSION   DASUGB010000362 DASUGB010000000
VERSION     DASUGB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875378"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFV87_06985"
     CDS             complement(<1..1316)
                     /locus_tag="VFV87_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5103538.1"
                     /translation="MHIAAFPQLAPDSTLADLPATNFRVTPDTWGNRITAEFDRQPDL
                     PGVLIVKEGELLGVISREAFLEHLSKPFALEVYMKRPIEVMLSQMDEQPLELPSTLGI
                     HAAADQTLNRPRHRVYEPIVVRHPGGDVRLLSSVVLLLAQSRLLALANETIQHQKEAA
                     DQANFAKSRFLANMSHEIRTPMNGIIGMTEIVLETQLLPEQREYLGLVRSSADWLLTV
                     INDILDFSKIEAGKLDLEEIEFDIRAMVAELLKTLEIRAQGKGLRLIGTVAPSVPRVL
                     IGDPVRIRQIITNLVGNAIKFTEQGEIEVRLEAGERAAGEVVLHGAVCDTGIGIPPDR
                     VGKIFEAFEQVDGSTTRKYGGTGLGLSISHRLVELMRGKIWAESALGQGSTFRFEVRL
                     KVAADDAAQRETVRPAAEAAAARPRARGLNVLLAEDNAVNQKLASLL"
     gene            complement(1332..>2105)
                     /locus_tag="VFV87_06990"
     CDS             complement(1332..>2105)
                     /locus_tag="VFV87_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5103539.1"
                     /translation="IGEQQPDIVVTDLQMPEMDGLQLVTAIRIHFPRVPVVLITAHGS
                     EELAVRALEQGAASYVPKSQLAANLQSVVDQVLELAKGNRGYEALAQSMDYAEFRFRA
                     DNDLATTDRLVELIQQLVGSIGVCDTGGQVRLGMALEEAFRLAVLRGNLEMTPEMLLA
                     ASGHGEEARTWLDKRRQSEPYASRRVLIHVKITPDEAHFTIGHEGPPLALADAGAAAE
                     NPALENPSDRSLILMRAFLDEVEFDPSGTSMKLVKRRSA"
BASE COUNT          322 a          678 c          721 g          384 t
ORIGIN      
        1 agcaggctgg ccagcttctg attgacggca ttgtcctctg ccaggagcac gttcagcccg
       61 cgagcccgcg gacgcgccgc ggctgcttcc gctgcgggcc gcacagtttc gcgctgggcc
      121 gcatcgtccg ccgcgacttt cagccgtact tcgaagcgaa acgtcgagcc ctgcccgagg
      181 gcgctctcgg cccagatttt gccgcgcatc agctcgacga ggcggtgcga gatcgagagc
      241 cccaggccgg tgccgccgta tttgcgcgtc gtgctcccat cgacctgctc gaaggcttcg
      301 aagattttgc cgacgcggtc cggcgggatg ccgatgcccg tatcgcacac ggcgccgtgc
      361 agcaccacct cgccggcggc ccgctcgccc gcttccaggc ggacctcgat ctcgccctgc
      421 tcggtgaact tgatcgcgtt gcccaccagg ttggtgatga tctggcggat gcggaccggg
      481 tcgccgatca gaactcgcgg cacgctgggg gccaccgtgc caatcagccg cagccccttg
      541 ccctgcgccc ggatttcgag tgtcttgagc aactcggcga ccatcgcccg gatgtcgaac
      601 tcgatctctt cgaggtcgag cttgccggcc tcgatcttgg agaagtcgag aatgtcgttg
      661 atgaccgtga gcagccaatc ggcggagctg cgcaccaggc ccaggtattc gcgctgctcg
      721 ggcaagagct gcgtctccag gacgatctcg gtcatgccga tgatcccgtt catcggcgtg
      781 cggatttcgt ggctcatgtt ggccaggaag cggctcttgg cgaagttggc ctggtcggcg
      841 gcttccttct ggtgctggat cgtttcgttg gccagggcca gcaggcgcga ctgtgccagg
      901 agcagcacta ccgagctgag caatcgcacg tcgccgccgg gatggcggac gacgatcggc
      961 tcatagacgc ggtggcgggg ccgattcagc gtctggtcgg cggcggcgtg gatgccaagc
     1021 gtggagggca gctccagcgg ctgctcgtcc atctgcgaga gcatcacctc gatcggccgc
     1081 ttcatataca cttcgagcgc aaacggcttg ctgaggtgct ccaggaacgc ctcacgcgag
     1141 atcacgccga gcagctcgcc ttctttcacg atcagcacgc cgggcaaatc gggctggcgg
     1201 tcgaattcgg ccgtgatgcg gttgccccac gtatcgggtg tgacgcggaa attcgtcgcc
     1261 ggcaggtcgg cgagcgtcga atcgggggcg agttggggga aggcagcgat gtgcacgctg
     1321 gttctccacg atcaagcgga ccggcgcttg acgagcttca tcgacgttcc gctgggatcg
     1381 aactcgacct catcgagaaa ggcccgcatc aggatgaggc ttcggtcgct ggggttttcc
     1441 agggcggggt tctcggcggc cgcgccggca tcggccagcg ccagcggtgg gccttcgtgg
     1501 ccgatggtga agtgcgcctc gtcgggcgtg attttgacgt ggatcagcac gcggcggctg
     1561 gcgtagggct cgctctggcg gcgcttgtcg agccaggtac gggcctcttc gccgtgaccg
     1621 gacgcggcca gcagcatctc aggcgtcatc tccaaattgc cgcgcaggac ggccagccga
     1681 aacgcttctt ccagggccat ccccaggcgc acctggccgc cggtgtcgca cacgccgatg
     1741 ctgccaacga gctgctggat gagctcgacg aggcggtcgg tggtggcgag gtcgttatcg
     1801 gcccggaagc ggaactcggc ataatccatc gactgggcga gggcttcgta gccgcggttg
     1861 cccttggcca gctccagcac ctgatcgacg acgctctgca ggttcgcggc gagctgcgac
     1921 ttggggacgt acgacgcggc tccctgctcc agggcccgca ctgccaactc ttcgctgccg
     1981 tgagcggtga tcagcaccac cggcacgcgc gggaaatgga tccggatggc ggtgacgagc
     2041 tgcaagccgt ccatctcggg catctgcagg tcggtcacga cgatgtcggg ctgctgctcg
     2101 ccgat
//
LOCUS       DASUGB010000363         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_875417, whole genome shotgun sequence.
ACCESSION   DASUGB010000363 DASUGB010000000
VERSION     DASUGB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875417"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2591
                     /locus_tag="VFV87_06995"
     CDS             <1..>2591
                     /locus_tag="VFV87_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5103540.1"
                     /translation="LRTWPQNPIDYFVLARLEAAGLSPSLPADKLALIRRVYLDLVGL
                     PPTPEEADAFVAACASDEHGGTERRRDIEKKALEAVVDKLLASPAYGERWARRWLDLA
                     RYADTNGYEKDRVRTMWLYRDWVINALNADMPFDQFTVEQLAGDMLPDCTLDQRIATG
                     FHRNTMLNEEGGIDPLEFRFYSMVDRANTTATVWMGLTLGCAQCHTHKFDPIPHADYY
                     KQLAFLNNADEPTIDVPQPDIAARRAEIERQIAEITANLPNRFPPEGDLKWHTPKPAS
                     FVSAGGASGQIRDDGSVLLSGTNPERDTYTLTIDSPPGEFSALRLEALVDPALPSTGP
                     GRTPHGNFVLSEVTLQLDQPVKFSRAEADFSQDQFPAAKAIDGDPKTGWAIHGPGKWN
                     VNRTLTLHFDKPVTLKEPGRWTITLDQQHGMQHTLGCLRISLAEPVRDDRPLEVRRKA
                     HLEQKFQAWLAAERKRTVKWTPLRPLAATSEIPILTIQDDGSIFVSGDMSKRDVYDIT
                     LDAGGLTGITALRLETMTDDRLPQSGPGRIYYEGPFGDFFLSRFTLTRSVSEGSSPLS
                     SRGRGAEGEGAEPPAIVRFQSASHSFANGNNTAAMAIDDDPQTGWSINGGQGRPHYAV
                     FQFEKPQEAADRLQLQMLFERYYAAGLGRFRIWATTDPRGGQARDFSNEIEELLLLPP
                     DQLTAGQTQKLLAQFCQVAPELAGEREAIKKLRDSLPAYPTALVMKEWPATRERATFV
                     HHRGEFLQPKDPVEPGLLQILSPLAKRQPQNRLEYARWLVSDANPLVGRVTMNRHWAA
                     FFGTGLVRTTEDFGYQGEPPSHEGLLDWLAVELPRRDWSLKSMHRLIVTSHTYRQSAE
                     FGVRNAE"
BASE COUNT          501 a          906 c          775 g          409 t
ORIGIN      
        1 gctaagaaca tggccgcaga acccgatcga ttacttcgtc ctggcgcggc tcgaagcggc
       61 cggcctttcc ccgtcgctcc cggccgacaa gctcgcgctc atccgccgcg tttatctcga
      121 cctggtcggt ctgccgccga caccggagga ggccgacgcg tttgtcgccg cctgcgcgtc
      181 agacgaacac ggagggacgg agagacggag agacattgaa aaaaaagcct tggaggccgt
      241 tgtcgataaa ctgctcgctt cccctgccta cggcgagcgc tgggcccgcc gctggctcga
      301 cctggcccgc tacgccgaca ccaacggata cgagaaggac cgcgtccgca cgatgtggct
      361 ctatcgcgat tgggtcatca atgccctcaa tgccgatatg ccgttcgatc agttcacggt
      421 cgagcagctc gccggcgaca tgctgcccga ttgcacactc gaccagcgga tcgccaccgg
      481 ctttcaccgc aacacgatgc tcaacgaaga ggggggcatc gacccgctgg agttccgctt
      541 ctattcgatg gtcgatcggg cgaacacgac cgcgaccgtt tggatgggcc tgacgctagg
      601 gtgtgcccaa tgccacacgc acaaattcga cccgattcca cacgccgact attacaagca
      661 gctcgccttc ctcaataacg ccgacgagcc gacgatcgac gtcccgcagc cggacatcgc
      721 cgcccgccgc gccgagatcg agcggcagat tgcggagatc acagccaatc ttcccaatcg
      781 cttcccgccg gagggcgacc tgaagtggca cacccctaag ccggccagct tcgtttccgc
      841 cgggggcgcg agcgggcaga ttcgcgacga tggctccgtg ctgctcagcg ggacgaatcc
      901 cgagcgggat acgtacacgc tgacaatcga ctcgccgccc ggcgagttct ccgccctgcg
      961 gctggaagcg ctcgtcgatc cggcgctccc cagtaccggc cccgggcgaa cgccgcatgg
     1021 aaacttcgtc ctcagcgagg tcacgctcca gctcgaccag ccggtcaaat tcagccgcgc
     1081 cgaagccgac ttctcgcagg atcagttccc cgctgccaaa gcgatcgatg gcgacccgaa
     1141 gaccggctgg gcgattcacg gccccggcaa atggaacgtc aaccgcacgc tcacgctcca
     1201 tttcgacaag ccggtcacgc tgaaggaacc gggccgctgg acgatcacgc tcgaccagca
     1261 gcacggaatg cagcacacgc tcggctgctt gcggatcagc ctcgcagagc cggtgaggga
     1321 cgatcgtccg ctggaagtcc gccgcaaagc ccacctggag cagaaattcc aggcctggct
     1381 cgccgccgag cgcaagcgga ccgtgaagtg gacgccgctc aggccgcttg cggcgacgag
     1441 cgagattccg attctcacga tccaggacga cggctcgatc ttcgtcagcg gcgatatgag
     1501 caagcgcgat gtctacgaca tcacgctcga tgccggcggc ctgaccggca tcaccgccct
     1561 gcgcctcgaa acgatgaccg atgatcgtct cccccaatcc ggccccggcc gcatctacta
     1621 cgaaggcccc tttggtgatt tcttcctgag ccgattcaca ctaacccgaa gcgtcagcga
     1681 gggttcctct cccctctcct ctcgtgggag aggggccgag ggtgaggggg cagaaccacc
     1741 ggcaatcgtc aggttccagt ccgcctccca ctccttcgcc aacggcaaca acacggccgc
     1801 gatggccatc gacgacgacc cgcagaccgg ctggtcgatc aacggcggcc agggccggcc
     1861 gcactacgcg gtattccaat tcgaaaagcc gcaggaagcc gcggatcgcc ttcaactgca
     1921 gatgctattt gagcgctact atgccgccgg tctcggccgc tttcgcattt gggcgacgac
     1981 cgatccccgc ggggggcagg ctcgcgactt ctcgaacgaa atcgaagaac tgctgcttct
     2041 gccaccggac cagctcacgg caggacaaac gcaaaaactc ctggcccagt tctgccaggt
     2101 cgcgccggaa ctggccggtg agcgggaagc gatcaagaag ctccgcgact cgctgcccgc
     2161 ttatcccacg gcgctggtca tgaaggagtg gccggcaacc cgcgagcggg ccacgttcgt
     2221 tcatcaccgc ggcgaattcc tgcagccgaa ggacccagtc gagccgggcc tgctgcagat
     2281 cctctcgccg ctggccaagc gccagccgca gaaccggttg gagtatgccc gctggttggt
     2341 cagcgatgcc aacccgctcg tcggtcgcgt cacgatgaac cgccactggg ccgccttctt
     2401 cggcacgggc ctcgtccgca ccaccgagga tttcggctat caaggcgagc cccccagcca
     2461 cgagggcctg ctcgactggc tcgccgtcga actgccccgc cgcgactggt cgctgaaatc
     2521 catgcaccgg ctgatcgtga cgagccacac gtacaggcag agtgcggaat tcggagtgcg
     2581 gaatgcggaa t
//
LOCUS       DASUGB010000364         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_458042, whole genome shotgun sequence.
ACCESSION   DASUGB010000364 DASUGB010000000
VERSION     DASUGB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458042"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..746)
                     /locus_tag="VFV87_07000"
     CDS             complement(195..746)
                     /locus_tag="VFV87_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX5103541.1"
                     /translation="MNGRELIVGVTGGIAAFKTAALVSHLVQDGCGVTVVMTAAAERF
                     IGAATFAALTGRPVAREVFDEAQYPLGAHIALAERGELLVVAPASADFLAKAAHGLAD
                     DLLSTLYLAFNKPVLMAPAMNTAMWEQPAVQRNIAQLRTDGVQFVDPESGWLSCRQVG
                     AGRMAAPETIVSAINALLGKERK"
     gene            complement(830..1093)
                     /locus_tag="VFV87_07005"
     CDS             complement(830..1093)
                     /locus_tag="VFV87_07005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5103542.1"
                     /translation="MLEDLKEESIVQKVGGRFKLSTLIQKRLVQLQAGSRPLVESSTQ
                     DKMQVVLQEIIQDKIYLDTTNQVRVVGEYQGTGEAMDLDLSDL"
     gene            complement(1112..1696)
                     /gene="gmk"
                     /locus_tag="VFV87_07010"
     CDS             complement(1112..1696)
                     /gene="gmk"
                     /locus_tag="VFV87_07010"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5103543.1"
                     /translation="MPLPDATKLGKMVVISGPSGAGKSTIVRRLLQVCPLPLIVSVSA
                     TTRKPRSGEVEGIDYHFLSPEEFARRREAGEFLECMDYAGNWYGTLQSQVTSGLAAGK
                     WVLLEIDVAGTIAVIEKHPEALTIFLHSGSIEELERRLVERKTETRETIERRLAVARR
                     ELEQAHRYRYQVINRDVEQAVRDICDILVHHAEN"
     gene            complement(1744..2664)
                     /locus_tag="VFV87_07015"
     CDS             complement(1744..2664)
                     /locus_tag="VFV87_07015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5103544.1"
                     /translation="MVAANHVKEPSALLLSMTGHGEAHRHDPSLSVAIEIRTVNNRYF
                     KLNIRTSEGYILLEPQIEAVVRQHVRRGTVQLNLQIGREPSADDYKLNEAVLRGYLMQ
                     LERLTGRSLADQPASLPPLLALPGVVHEPSAQLDVIESQWPVIESVLTAALERLAKMR
                     AAEGAAMAADLSANAQTIAVELAAVERRAPEVVAAYRARLLERLQVLLADLGVQIQPA
                     DVVREVGVFAERTDISEEIVRLRSHLEQFAAVLASAESQGRKLDFVIQEMFRETNTIG
                     SKANDAEIARHVIEMKSAIERMREMIQNVE"
     gene            complement(2676..3143)
                     /gene="secG"
                     /locus_tag="VFV87_07020"
     CDS             complement(2676..3143)
                     /gene="secG"
                     /locus_tag="VFV87_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5103545.1"
                     /translation="MILAGVDWLHYAFMIPMALLSVLLILVILVQRGRGGGLTGALGG
                     MGGASAFGTKAGDLFTKITIVLALVWVLLAMASLKILNQRSPFSTAGGNQPATTVPSD
                     PTATPAGGAKTPAPDGASSSVPKSSPAAAPKSSPSPASSSGSKSSEKTPPATP"
     gene            complement(3198..>3515)
                     /locus_tag="VFV87_07025"
     CDS             complement(3198..>3515)
                     /locus_tag="VFV87_07025"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5103546.1"
                     /translation="EGSLAGLTPEQAVKLVIAYEPVWAIGTGVVATPEQAEAVHADLR
                     RLLETRYNSEVASSVRIQYGGSVNAENASSLLGQPNIDGALVGGASLKADGFLAIIAA
                     ARG"
BASE COUNT          612 a         1136 c         1092 g          675 t
ORIGIN      
        1 ccgtagagcg acagcaggaa gcgatacagg cggacctgca gacgggcaag ccacgcgggg
       61 cggccggtgg aggtggtatc cgcggcctcg gcggcggcga ggcggtcatg ccaatcggcg
      121 atcagcttgt cgcgcgcggt aggcattgcg cccgcctcca tctttcacca gcaatcaatt
      181 acccgctcat tttcctattt ccgctctttg ccaagcaagg cgtttatcgc tgagacgatt
      241 gtctcgggcg cggccatccg gccggcgccg acctggcggc agcttagcca gccggattcg
      301 ggatcgacga actgcacacc gtccgtccgg agctgggcga tattgcgctg cactgccggc
      361 tgctcccaca tggccgtgtt catcgcgggg gccatcagga ccggcttgtt gaacgccaga
      421 tagagcgtcg aaagcaggtc atcggccagg ccgtgggcgg ctttggccag gaagtcggca
      481 ctggcgggag cgacgaccag cagctcgcct cgctcggcca gggcaatgtg ggccccgagc
      541 ggatactggg cttcgtcgaa gacttcacga gcgaccggcc ggccggtgag ggcggcgaag
      601 gtcgccgcgc cgatgaaccg ctcggcagcc gcggtcatga cgactgtcac gccgcagccg
      661 tcctgcacga gatggctgac gagcgcggcc gtcttgaacg cggcgatgcc gcccgtgacc
      721 ccgacaatga gctcgcgacc gttcatcgcg tcagtccaaa gtccaatgtc caatgtccaa
      781 ggttgatcgc tgcgactttg gactttggac cttggacagt ttgattcgcc tacaaatcgc
      841 tgaggtcgag atccatcgcc tcgcctgtcc cctgatactc gccgacgacg cgaacctggt
      901 tggtggtgtc gagatagatt ttgtcctgga tgatctcctg cagcaccacc tgcatcttgt
      961 cctgcgtgct cgattccacg agcggtcggc tgccggcctg aagctgcacc agccgcttct
     1021 ggatgagcgt gctgagcttg aagcgtcctc cgaccttctg gacgatgctt tcctctttca
     1081 gatcttcgag catattcact ccttggcgag ttcagttttc ggcgtgatga accagaatgt
     1141 cgcagatgtc gcggacggcc tgctcgacat cgcgattgat aacctgataa cggtaacggt
     1201 gggcttgctc cagttcgcgg cgggccaccg ccaggcggcg ctcgatcgtt tcgcgggtct
     1261 cggtcttgcg ttcgaccaga cgccgctcca gttcttcaat cgagccgctg tgtaggaaga
     1321 tggtgagggc ctcgggatgc ttctcgatga ccgcgatcgt gccggcgacg tcaatctcta
     1381 aaagtaccca ctttcccgcc gcaagtccag aggtgacttg gctttgcagc gtgccgtacc
     1441 agttgcccgc atagtccatg cattccagaa actcacccgc ttcgcggcga cgggcgaact
     1501 cctccggcga cagaaagtgg tagtcgatgc cctccacctc gccacttcgg ggcttgcgag
     1561 tcgtggccga aacgctgacg atcagcggca gcgggcagac ttgcagcagc cggcggacga
     1621 tcgtcgattt ccccgccccc gatgggccgg agatgacgac cattttcccg agcttggtgg
     1681 cgtctggcag aggcatgcct gaagaactgg cttttggttc ttcggttctt gtttcttgac
     1741 tattcactcg acgttctgaa tcatctcccg catccgttcg atcgccgatt tcatctcgat
     1801 cacgtggcgg gcgatttcgg catcgttggc tttggagccg atcgtgttcg tttcgcggaa
     1861 catttcctgg ataacaaagt ctagcttgcg gccctgcgac tcggcggatg ccaggacggc
     1921 ggcgaactgc tccaggtggc tgcggagccg cacgatttcc tcagaaatat cggtccgctc
     1981 ggcaaagacg ccgacttcgc ggaccacgtc cgccggctgg atttggacgc ccagatcggc
     2041 caacagcacc tgcagccgct ccaagagccg ggcgcggtaa gccgccacga cctccggcgc
     2101 ccggcgctcg acggcggcga gttccaccgc aatcgtctgg gcattggcgg acagatcggc
     2161 cgccatcgcg gctccttccg cggcacgcat cttcgccagg cgctccagag ccgcggtcag
     2221 cacgctctca atcaccggcc actgggactc gatcacatcg agttgggcgc tcggctcgtg
     2281 gacgacgcct ggcagcgcca agagcggcgg cagcgaggcg ggctgatcgg ccagcgagcg
     2341 gcccgtcaac cgctccagct gcatcaggta gccgcgaaga accgcctcat tcagcttgta
     2401 gtcgtccgcc gagggctcgc ggccgatctg cagattcaat tgcacagtgc cgcggcggac
     2461 gtgctgccgc acgactgcct cgatctgcgg ctccagaagg atgtagccct cggacgtgcg
     2521 gatattcagc ttgaagtagc ggttgttgac cgtgcggatt tcgatcgcga ccgacaggct
     2581 cggatcgtgg cggtgggcct ctccatgacc ggtcatgctg aggagcaagg cggacggctc
     2641 cttgacgtga ttcgcggcga ccacgcgccg ggcgattagg gcgtcgctgg cggagttttc
     2701 tcggacgatt tcgacccgct ggaggatgcc ggagatggcg aagacttcgg agccgcagcg
     2761 ggtgagctct tcggaacgct cgatgatgcg ccgtcggggg cgggcgtttt ggcaccaccc
     2821 gcgggagtcg cggtcggatc gctcggcaca gtcgtcgccg gctggtttcc tccagccgtg
     2881 ctgaacggcg agcgctgatt caggatcttt aagctcgcca tcgccagcag cacccacacg
     2941 agcgccagga cgattgtgat ttttgtaaac aggtcgccgg ctttggtgcc gaaggcgctc
     3001 gctccgccca tgccgcccaa ggcgccggtc aaaccgccgc cgcgcccgcg ctggaccagg
     3061 atcacaagga tcagcagcac cgagagcagt gccatcggaa tcatgaacgc atagtgcagc
     3121 caatcgacgc cagccagaat catcacagtt cgctccttaa atcctttcgc tcctgcacct
     3181 tacaatcggc ttatcgatca gccgcgggcg gcggcaatga tcgccagaaa accatccgcc
     3241 ttcagcgacg ccccgccgac tagagccccg tcaatgttcg gctggccgag caggctggac
     3301 gcgttctccg cgttcacact gccgccgtac tggatccgga cacttgacgc aacttctgaa
     3361 ttgtaacggg tttccagcaa cctgcgaagg tcagcatgca ccgcctctgc ctgctccggc
     3421 gtggccacga cgcccgtccc aatagcccac accggctcat aagcgatgac cagcttcacc
     3481 gcctgctcgg gcgtcagccc ggcgagcgag ccctc
//
LOCUS       DASUGB010000365         5078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_595892, whole genome shotgun sequence.
ACCESSION   DASUGB010000365 DASUGB010000000
VERSION     DASUGB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595892"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1520)
                     /locus_tag="VFV87_07030"
     CDS             complement(327..1520)
                     /locus_tag="VFV87_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5103547.1"
                     /translation="MTTNGSLNRKFRMALVGGGQGAFIGRVHATAAVMDNRAALVAGC
                     LSSSPEKAKASAPDYDIPADRAYTSIKELIEKEKALPADKRIDFVSVATPNHTHFEIA
                     KAAADAGFNVMCDKPMTFDFAQAEALAEAVDKSGVVFAVTHNYTGHPLVRQARDMILN
                     GELGEINAIRSCYIQGWLRTRLEKDSQKQASWRTDPSKSGAAGCYGDIGTHAYNLARY
                     MTGLLPDQISAHLKTFEQGRQLDDYGTAIIKFQNGALGTVTASQITHGRENDIFIEID
                     GTKGSLEWHQENPNQMFVRTNGQPAKVYTRAGGPYLSAAAAAATRIPAGHPEGYLEAF
                     ANLYRSAYDDMVLRAEGKKFERVNTVYPNVNDGLEGMYFIQQAVASSQQGGAWLPLAH
                     KRARK"
     gene            1767..2204
                     /locus_tag="VFV87_07035"
     CDS             1767..2204
                     /locus_tag="VFV87_07035"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HEX5103548.1"
                     /translation="MSQFPNQPGYGPNPFGQQPGPYQPPKSSKAWLWILLGVGGVGLL
                     VCCGCGGLFLVGMGVVGNQLVAQLNADPNVQQHLGTVTSANVDWMATGEEGNKAPGKS
                     VMVFNVVGEKGSAKVIVEQTPGQQHFNNGRLRLPSGEEIPLSF"
     gene            2586..>5078
                     /locus_tag="VFV87_07040"
     CDS             2586..>5078
                     /locus_tag="VFV87_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103549.1"
                     /translation="MSRHWPLLSLPLFTLFAFSCLAPVIAQEPKPPAPTAEQIAAAEG
                     QQPLREQTIYIPYAKLRALFEKEGRGVFVPYDEFQRMWKAARDATKKLEEYKPPIGAL
                     IAEIDSEATVSKDVMNVQARLQIEVLTEGWHEVPLRLAHAAIRNAKIGDQPARLIYSP
                     DTGYRLLLEKKGKAAERVELVLEYSRAFTKQPGTNRVEFDAPQAPVNRWQIRLAEPGV
                     KVNVHPNISTTDAGAEQMGDAAPDPAAAKPAEKETVVQAFVGAADVVRIDWTAKAEGA
                     AGLAALVTVQARQEVTIDEGVVRTRANLAYDITRADVTQLTVEVPADHNVVNVFDPNV
                     QKWEKKTEGPLQTLTISLYQPTRGAQNILIELEKFAGDKEMPQEMMHAEIKAPVVRAV
                     AAGTPEAMTNIGRQQGVVVVRLGTSLRGEVPSRTGLLQIDAAELPAPLAGQEWTFAYR
                     YAALPFDLQLSVEKLLPQIEVEELVETYVEPNQITIDLLSILNIERAGIFQIELEVPE
                     GYEIRTVQGRDAAGAAAVAVDSYHLDEVEYVPDPTMPEVKAKKRTKLLVNLSRRALGR
                     VALWASLQKRQEDANLLQPTGATSVINLPVPRVQPASVARTSGRLLVYAPESLRINPQ
                     EVKGLRPISPTEAVANVGSTRDGRFAGLNPLVAYAYTQEPASLIVEGTRRKPYIEARQ
                     LLSAHIESGVVRYEATLFYDIKYSSVKTLRIDVPAALAADIRNLTPALREQRIEPQPG
                     DVAQDYVAWQLAGEGELIGSVAVKFQWEITLPELKEGDTAKIELPALVAIGVDRFWGQ
                     ITASKAESIEIAPDEGEKGLRGIDPQRDLMPGA"
BASE COUNT          898 a         1520 c         1740 g          920 t
ORIGIN      
        1 caggctcgtg cgatttgcca aaggcggcgg cagctacctc tcgtccagcg atcgccgctt
       61 gctctttggc ttcgatgatc gtgagggccc ggtccgcgtc agtgttcgat ggtcgtgggg
      121 caattctcaa acctgggaga atctgcagcc aggtgcttac taccggttgc gtgaaggcga
      181 cccgtttgcg gatctcgcag accaactgag ctccgggcgc tgacctcgat tgacgatcag
      241 gcccgggaca tgctgcccca tcgccgatcg gaaacccggg cacaagccag gcgctgcaat
      301 cggccgcgcc ttgcagagcg acaaggctac ttccgagccc gcttgtgggc cagcggcagc
      361 caggcgccgc cttgctggct gctggcgacg gcttgctgga tgaagtacat cccttccagg
      421 ccgtcgttga cgttgggata gaccgtgttc acccgctcga acttcttgcc ttcggcgcga
      481 aggaccatgt cgtcgtaggc ggagcggtag aggttggcga aggcttccag atagccctcg
      541 ggatggccgg ctggaatgcg ggtggcggca gcggcggcag cgctgagata cgggccgccg
      601 gcgcgggtat agaccttcgc cggctggccg ttggtgcgga cgaacatctg gttggggttt
      661 tcctgatgcc actcgagcga gcccttggtg ccgtcgattt cgatgaagat gtcgttctcg
      721 cggccgtgag tgatctgcga ggcggtgacg gttcccaggg ccccgttttg gaatttaatg
      781 atcgcagtgc catagtcgtc gagctgccgg ccctgctcga aggtcttgag gtgggcgctg
      841 atctggtcgg gcaacaggcc cgtcatgtaa cgggcgaggt tgtacgcatg ggtgccgata
      901 tcgccgtagc agccggccgc gccgctcttg ctgggatcgg tccgccagct cgcctgcttt
      961 tgtgagtctt tttccaggcg cgtgcggagc cagccctgga tgtagcagga gcggatcgcg
     1021 ttgatttcgc ccaactcgcc attcaggatc atgtcgcggg cctggcgcac cagcggatgg
     1081 cccgtgtagt tgtgcgtgac ggcgaagacg actcccgatt tatcgaccgc ttcggccaaa
     1141 gcttcggcct gggcgaagtc gaaggtcatc ggcttgtcgc acatgacgtt gaagccggca
     1201 tccgctgcag ccttggcgat ttcgaagtgc gtatggttgg gcgtggcgac gctgacgaag
     1261 tcgatccgtt tgtcagcggg aagcgccttt tccttctcga tcagctcctt gatcgaggta
     1321 taggcgcgat cggcgggaat gtcgtagtcc ggggcgctgg ccttggcctt ttccggactg
     1381 ctggagaggc agccggcgac gagcgcggcg cggttgtcca tgacggccgc tgtggcgtgg
     1441 acgcggccga tgaaagcgcc ttggccgcct ccgaccaggg ccatgcggaa tttgcggttg
     1501 agtgagccgt tggtggtcat ggagagaatt cctttgcgtg ctgcccgttg gccaatctgc
     1561 gcttggtgat cgggcccgtg cggacgaggt tctttgttgt agcaaatcgc gcggcggacg
     1621 ccagttgccg gcagggcatc agtcattctt gtctgccggc aggcactgcc ccacgttgga
     1681 ccttcccccg ttgtcggcgt gtgtgcgaat cgccacactt ttggagacgc ttgtcgttgc
     1741 tcacgccttt cctggaaccc tgtcctatgt cacagtttcc caaccagccc ggctacggtc
     1801 ccaatccctt cggccagcag cccgggccct accagccgcc gaaatcgtcg aaggcctggc
     1861 tgtggatatt gctgggtgtg gggggcgtgg gcctgttggt ctgctgcggg tgcggtggat
     1921 tgtttcttgt cggcatgggc gtggtaggca atcaattggt cgcgcagctc aacgccgatc
     1981 caaatgtcca gcagcatttg ggcaccgtga caagcgccaa cgttgattgg atggccaccg
     2041 gcgaggaggg aaacaaggct cccggcaaga gcgtgatggt gttcaacgtg gtcggcgaaa
     2101 agggctcggc aaaagtgatc gttgagcaga cgccggggca gcagcatttc aacaacggcc
     2161 ggctccgtct ccccagcggc gaagagattc ccctgtcatt ttgagggaga aggtggagtt
     2221 gggtcgggtt gggcaacaag aaggaaaggg ccaccggatc gaacagttca ttgaattgtg
     2281 ccggatgaat cgcctatagg tccttctgca tgcatggcgt cgtagccgac gccggttgga
     2341 cgggcggaat tggccggcac gcgccggcca tgagcaagaa ttctgccggg tcccagccgt
     2401 gctgtcttac ggccgccctt tacaaatccg ttacgggcgg tgctctcgcc tgagaacaat
     2461 ggccaggcga gcatcgtcta actttacgct ctggtggtct gcgcagtcgg cgcggaccgt
     2521 aaaggggtga ttcgccgctg ctgggcggcg ctcgagtttc gacgtttcgt agcaaaggac
     2581 cgcgcatgtc ccgccactgg ccgctcctct cattgcccct ctttacgctt tttgccttct
     2641 catgcctggc cccggtgatc gctcaagagc ccaagccgcc ggcgccgacg gcggagcaga
     2701 ttgccgcggc ggaaggccag cagccgctgc gcgagcagac gatctacatc ccgtacgcca
     2761 agctgcgggc gctctttgaa aaggaaggcc gcggcgtctt cgtgccgtac gacgaattcc
     2821 agcggatgtg gaaggcggct cgcgacgcga cgaagaagct cgaagagtac aagccgccga
     2881 tcggcgcgct gatcgctgag atcgacagcg aagcgaccgt gagcaaggac gtgatgaacg
     2941 tccaggcccg cctgcagatc gaagtgctca ccgaaggctg gcacgaagtg ccgctgcgat
     3001 tggctcacgc ggccattcgc aacgcgaaga tcggcgatca gcccgcccgg ctcatttatt
     3061 ctccggacac cggctatcgg ctgctgctgg aaaagaaggg gaaggcggcg gagcgcgtgg
     3121 agctggtgct ggaatacagc cgggccttca ccaagcagcc ggggacgaat cgcgtcgaat
     3181 tcgacgcgcc gcaggcgccg gtcaatcgct ggcagattcg cctcgccgag ccgggcgtga
     3241 aggtgaacgt ccatccgaac atctcgacga ccgacgccgg cgccgagcag atgggcgacg
     3301 ccgctcccga cccggcagcc gctaagccgg ccgagaaaga gacggtcgtg caggcgttcg
     3361 tcggcgcagc cgatgtcgtg cggatcgact ggaccgccaa agccgaggga gccgccggct
     3421 tggcggcact ggtcaccgtg caggcccggc aggaagtgac gatcgacgaa ggggtcgtcc
     3481 gtacgcgagc caacctggcc tacgacatca cgcgggccga cgtgacgcag ctcaccgtcg
     3541 aagtgccggc cgatcacaac gtggtcaacg tcttcgatcc caacgtgcag aagtgggaga
     3601 agaagaccga aggtccgctg cagacactca cgatttcgct ttaccagccg acgcggggcg
     3661 ctcagaacat cctgatcgag ctggaaaagt tcgccggcga caaggaaatg ccgcaggaga
     3721 tgatgcacgc cgagatcaag gccccggtcg ttcgcgccgt ggctgccggc accccggagg
     3781 cgatgacgaa catcggccgc cagcagggcg tcgtggtcgt gcggctgggg acgtcgctgc
     3841 ggggagaagt tccttcgcgg acggggttgc tgcagatcga cgccgcggag ttgcccgcgc
     3901 cgctggccgg gcaggagtgg accttcgcgt atcgctatgc cgccctgccg ttcgaccttc
     3961 aactgagcgt cgaaaagctg ctgccgcaga tcgaagtcga ggagctggtc gagacgtatg
     4021 tcgagccgaa tcaaatcacg atcgacctgc tctcgattct caacatcgag cgggccggca
     4081 tcttccagat cgagctggaa gtgcccgaag gctacgagat tcgcaccgtt cagggccgcg
     4141 atgcggcggg cgcggcggcg gtggcggtcg attcctatca cctcgatgaa gtcgagtatg
     4201 tgccagaccc caccatgccc gaggtgaagg ccaagaagcg gacgaagctg ctcgtgaatc
     4261 tctcgcggcg ggcgctcggc cgcgtggcgt tgtgggccag cctgcagaag cggcaggaag
     4321 acgcgaacct cctgcagccg acgggcgcga cctcggtgat caacctgccc gtgccgcgcg
     4381 tgcagccggc gagcgtggcc cgcacgtccg gccgcctgct cgtgtatgct cccgagagtc
     4441 tgcggatcaa cccccaggaa gtgaaggggc tgcggccgat ttcgcccacc gaggcggtgg
     4501 cgaacgtcgg ctcgacgcgc gacggccgct ttgccgggct caatccgctg gttgcgtatg
     4561 cctacacaca ggagccggcg agcctgatcg tcgaagggac ccgccgcaag ccgtatatcg
     4621 aggcccggca gctcttatcg gcacacatcg agtcgggcgt cgtccgctat gaggcgacgc
     4681 tcttctacga catcaaatac agcagcgtca aaacgctgcg gatcgacgtg ccggcggcgc
     4741 tggcggcgga catccgcaat ctgacgcccg ccctccggga gcagcgcatc gagccgcagc
     4801 cgggggacgt ggcccaggat tacgtcgcct ggcagctcgc gggcgagggg gagctgatcg
     4861 gcagcgtggc ggtgaaattc cagtgggaga taacgctgcc cgaattaaag gaaggcgata
     4921 cggccaaaat tgagctgccg gcgctcgtgg cgatcggcgt cgatcgcttt tgggggcaga
     4981 tcacggccag caaggcggag tcgatcgaga tcgccccgga cgaaggggag aaggggctgc
     5041 ggggaatcga tccgcagcga gatctgatgc ccggcgcc
//
LOCUS       DASUGB010000366         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1847155, whole genome shotgun sequence.
ACCESSION   DASUGB010000366 DASUGB010000000
VERSION     DASUGB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847155"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1141)
                     /locus_tag="VFV87_07045"
     CDS             complement(152..1141)
                     /locus_tag="VFV87_07045"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5103550.1"
                     /translation="MAQKQMQQEVQQAEKTAAEQRKIQQKIDAKPEPEEKKEAPQTGA
                     RTYPVPPFEKQHQPKPGSEEELEPQPMYDAPYWKGSGKLEGKVAIVTGGDSGIGRSVA
                     VLFAREGAEIAVIYLSEHEDAEQTRLAVADEGKDCLLISGDISHRDFCRKAIGQVIYK
                     FGRLDVLVNNAAFQLHVDRFEDLTQEHFDRTLKTNLYGMFNMSQEAVGYMEPGSAIVN
                     NGSVTGLMGNKELLDYSMTKGGIHAFTRSLATHLLPRGIRVNAVAPGPVWTPLNPADK
                     PADEVACFGADTPMKRPAQPEEIAPAFVFLASPQMSSYITGEILPVIGGYSGG"
     gene            1255..1332
                     /locus_tag="VFV87_07050"
     tRNA            1255..1332
                     /locus_tag="VFV87_07050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1290..1292,aa:Pro,seq:ggg)
     gene            1472..2731
                     /locus_tag="VFV87_07055"
     CDS             1472..2731
                     /locus_tag="VFV87_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5103551.1"
                     /translation="MALAIGFLVGIERGWKQRDEQDGERVAGLRTFALSGLFGGLTGL
                     TLPLAGPLFLAAVSLAFAALFILFQYLDAIETGTKSATAALVGLMVYGLGVYSALGDL
                     TVAAAVGVTVTLILAFKQTLHGWLKSLSWPEIRSALLILAATFIALPILPDRTIDPWE
                     AVNPRSLWFMTIVLAAATFGGYVALKVLGQKAGLFVGAVVGAIVSSTAVTLDLARRAK
                     AGEAPPRDAAAAAMLATVVSLTRVGIIGSAFSAGIALHLWIPLGAAILVSLAFAATIV
                     LIDRGATSESGERNIRNPLDVVEVGKFALLLAVLTVAAKLSAVWFGDAGLSVFAATAG
                     LVDVDAVTLAVGGMRNLSPTSAASAILIAAAANTLFKAGIAVATGGRAFGLWFGAACV
                     AALAAGAIAFAVSAAWVGEPGAGGGLP"
     gene            2780..2855
                     /locus_tag="VFV87_07060"
     tRNA            2780..2855
                     /locus_tag="VFV87_07060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2813..2815,aa:Val,seq:tac)
     gene            complement(2955..>3255)
                     /locus_tag="VFV87_07065"
     CDS             complement(2955..>3255)
                     /locus_tag="VFV87_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103552.1"
                     /translation="LQNLLHVQGLTIRGAIKVLDEEPGAVPASESGAETMMTLPPEEA
                     GASVRELQAAVQEAVERGDFRHAGELGEGARDRLESLLTDLSKLKSRLDAVRTAA"
BASE COUNT          473 a         1029 c         1106 g          647 t
ORIGIN      
        1 gccgcgtgat cgcgcagggc cagggagaga tgccgtcgct ggcggcagcg ctcagcccgg
       61 tcgaaatcga agcgatcgcc aagcacgtgg tgaagaccct cgggccccag cagcgcggcc
      121 caggccgtgg cggtccgcca ccggaggaga actagcctcc gctatagccg ccgatcaccg
      181 gcaggatttc gccggtgatg taggacgaca tctgcggcga ggcgagaaag acaaaggccg
      241 gcgcgatctc ttcaggttgc gccggccttt tcattggcgt gtcggcgccg aagcaggcga
      301 cctcgtccgc cggcttgtct gcgggattga gcggcgtcca gaccgggccc ggcgcgacgg
      361 cattcacacg gatgccgcgc ggcaggagat gcgttgccag cgagcgggtg aaggcgtgga
      421 tgcctccctt ggtcatcgaa tagtcaagca gctccttgtt gcccatgagg ccggttaccg
      481 agccgttgtt cacgatggcc gagcccggct ccatgtagcc cacggcctcc tgggacatgt
      541 tgaacatgcc gtagaggttg gtcttaagcg tacggtcgaa atgttcctgc gtcaggtctt
      601 cgaagcggtc gacatggagc tggaaggccg cgttgttcac cagcacgtcg aggcggccga
      661 acttgtagat cacctgcccg atggccttgc ggcagaaatc gcggtgggag atgtctcctg
      721 agatcagcag gcagtccttg ccttcatcgg ccacggcaag acgcgtctgc tcggcgtcct
      781 cgtgctcgct gagatagatg acggcgatct cggcgccctc gcgggcgaaa agtacagcga
      841 cggagcggcc gatgccgctg tccccgccag tgacgatggc gaccttgccc tcgagcttgc
      901 cggagccctt ccagtagggc gcatcgtaca tcggctgcgg ctcgagctct tcttccgaac
      961 ccggctttgg ttgatgctgc ttttcaaacg gcgggacggg atacgtgcgt gcgccggtct
     1021 ggggcgcttc cttcttttcc tcaggctccg gctttgcgtc gattttctgc tggatctttc
     1081 gctgctcggc tgcggtcttt tctgcctgct ggacttcctg ctgcatctgt ttctgggcca
     1141 tcgccgcatc tccgttctgg gccggtaaac cccgaacgga cggcgaaaaa ccttgttcca
     1201 gctgccgtgt tgcccatccc cggggaggcg cctataaggg cgctcctgaa ttggcggagc
     1261 gtggcgcagc ccggttagcg caccagtctg ggggactggg ggtcgtgggt tcgaatcccg
     1321 ccgctccgac cattcaggcg cttccccgaa atccgcctaa gacccgagtg catatcgcct
     1381 ttccggcgac ttgcggtgca ggccacggtc tcgcgcgtta tgcagcagca tggagaccgg
     1441 ggagcttttc gacgcccttc agcgcctcgc agtggcgctg gcgatcggat tcctggtggg
     1501 gatcgaacgc gggtggaaac agcgggacga gcaggacggc gaacgcgtcg ccgggcttcg
     1561 gacctttgcc ctatccggac tgtttggcgg cctgacgggc cttacgctgc cgctggcggg
     1621 tccgctgttc cttgcggcag tgagcctcgc cttcgcggcg ctgttcatcc tgttccagta
     1681 tctggatgcg atcgagacag ggacgaagtc ggcaaccgca gcgctcgtag gcctgatggt
     1741 gtacgggctc ggcgtttatt cggctttggg cgatctgacc gtcgcggctg ccgttggcgt
     1801 gaccgtgacc ttgatccttg cgttcaagca gacgcttcat ggctggttga aatccctgag
     1861 ctggccggaa atccgttctg cgctgcttat cctcgccgcg accttcatcg ccctgcctat
     1921 cctgcctgat cggaccatcg atccgtggga agcggtcaat ccacgttcgt tgtggttcat
     1981 gaccatcgtg ctggcggcgg cgacattcgg cggctatgtc gcgctcaagg ttcttggaca
     2041 gaaggctggg ctgttcgtcg gcgcggtcgt cggcgcaatc gtgtcctcga cggcggttac
     2101 gctcgatctg gcgcggcgcg caaaggccgg cgaagcgccg ccacgcgatg cggccgccgc
     2161 cgccatgctg gcgaccgtgg tgagcctgac gcgcgtaggg atcatcggct cggccttctc
     2221 cgccgggatc gcattgcatc tctggatccc cctgggagcg gcaatcctcg tgtcgctcgc
     2281 ttttgcagcg acgattgtgc tgatcgaccg cggcgcaacg agcgagagcg gcgagcggaa
     2341 tatccgcaac ccgctcgatg tcgtggaggt gggtaaattc gccctgctcc tcgccgtgct
     2401 gaccgttgca gcgaagctgt ctgccgtctg gttcggagat gcggggctca gtgtgttcgc
     2461 ggcgacggcg gggctggtgg atgtcgacgc cgttacgctg gctgtgggcg gaatgcggaa
     2521 cctttcgccg acctccgcag cctcggccat cctgatcgca gcggcggcta acacgctgtt
     2581 caaggcaggg atcgcggtag caaccggtgg cagggccttc gggctgtggt tcggggcggc
     2641 ctgcgtcgcg gcgctggcgg ctggggcgat cgcctttgcg gtttctgcgg cctgggtggg
     2701 cgagccgggc gcgggcggcg gcttgccctg atcgccagcc ttgaccggag gggcgggtgc
     2761 ggttagcgcg gggccctcag ggcggttagc tcagctggca gagcatctcg tttacaccga
     2821 gagggtcggc ggttcgatcc cgtcaccgcc caccagcctt cgcgcttcgc gctacggctt
     2881 ggcaagccag ccttgtagcg acttgtcgcg ctgtagcccg cagggcgaag gcgggccgtg
     2941 cgttgaggaa cagatcaggc cgcggtccgc actgcgtcca gccgcgattt cagcttcgac
     3001 aaatccgtca gcagggattc cagccggtcc cgcgcgccct cgcccagctc gccagcgtgg
     3061 cggaagtcgc cgcgctcgac cgcctcctga accgccgcct gcagctcgcg cacagatgca
     3121 ccggcttctt ccggaggaag agtcatcatg gtttcagccc cgctctcgct ggccggaacc
     3181 gcccccggtt cttcgtcgag cacctttatg gccccgcgaa tggtcagccc ctgtacgtgg
     3241 aggagattct gcagc
//
LOCUS       DASUGB010000367         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1847205, whole genome shotgun sequence.
ACCESSION   DASUGB010000367 DASUGB010000000
VERSION     DASUGB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847205"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VFV87_07070"
     CDS             <1..1543
                     /locus_tag="VFV87_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5103553.1"
                     /translation="EFGFDFLRDRLLLRRMGVSQAGIFGEISAARLSPGSEQPVQRGA
                     HFALVDEADSILIDEARTPLIIGSLGDKAVEKIVATYRWAAAAVPEFSDETHYEYDHD
                     EKKVELTATGRQLVRTLPKPELLASMGLIDLYQYVERAIKVDRDFHLDRQYVVKPGEQ
                     GKPEIVIVDENTGRLAEGRKWRDGIHQAIEAKERIDVTVPTGQAARITVQDLFLRYKH
                     LAGMTGTARTAAREFRKVYKKVVVPIPTNRRVQRQRLSDKVFGTGDQKWQAIVEEIRE
                     LNQLGRPVLVGTRSIDKSVHLSQLLDAVGIRHKVLNAHQVAVEAEIVAQAGRVGGVTI
                     ATNMAGRGTDIILGGNSQEMAWAKLHDKFPTKLDVPADAWKQAVEEIEQAEKLAEERA
                     KVRELGGLHVICTELHDSARIDRQLIGRCGRQGDPGTVRQYMSLDDDVILTGFGPETA
                     HKMELLGQQNGASAQRYLGLLKRAQRKVERRHLRDRFVLLYHEKERKKMQAEMGQDPY
                     LDTPD"
     gene            complement(1540..>2747)
                     /locus_tag="VFV87_07075"
     CDS             complement(1540..>2747)
                     /locus_tag="VFV87_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103554.1"
                     /translation="EEAPKGAPPKSLIVLYMEGGPSQLETFDPHPGTKFSHASTKNIK
                     TTAKDIEISDWLPQVAEQMEHISLVRSVFSKEGDHERAAYNIKTGWRPDPTLVHPSLG
                     AILCHQTLGQDKCEIPRHVSILPGPWAARGGYLGDQYDAFKTYDPNGQIPDITAPVSA
                     GRAARRLDDLENVVEKAFAQGRVKSLPEKTLHSVSIAAARKMMTSEQLKAFDVREAPE
                     SLKQEFGDTPFGRACLAAVQLVGVGVRCVEVTLSGWDTHVNNEELVKGRCDILDPAFA
                     TLIKQLQERDLFDSTVVLWGGEFGRSPTIRGLDGGGRDHWPHGFTMALAGGGIKGGRV
                     IGETAAEPKLDEKNRQQDVKGPRNVEDIHATVLQALGVDYTHEELTPIGRPMRFTMES
                     AQPIDELLV"
BASE COUNT          501 a          903 c          876 g          467 t
ORIGIN      
        1 ggagttcggc ttcgacttcc tccgcgaccg cctgctcctc cgccggatgg gcgtttcgca
       61 ggcgggcatc ttcggcgaga tcagcgccgc ccgactgtcc cccggcagcg agcagcccgt
      121 gcagcgcggc gcgcacttcg ccctcgtcga tgaagccgac agcattctga tcgacgaggc
      181 ccgcacgccg ctgatcatcg ggtcgctggg ggacaaggcg gtcgagaaga tcgtggccac
      241 ctatcgctgg gccgcggccg ccgtaccgga attcagcgac gaaacgcact acgagtacga
      301 tcacgacgag aagaaggtcg agctcaccgc gaccggccgc cagctcgtcc gcacgctccc
      361 aaagcccgag ttgctggcga gcatgggcct gatcgacctc tatcagtatg tcgagcgggc
      421 catcaaggtc gatcgagatt tccacctcga ccgccagtac gtcgtcaagc caggtgagca
      481 gggcaagcca gaaatcgtca tcgtcgatga gaacaccggc cgcctcgccg aaggccgcaa
      541 gtggcgcgac ggtatccacc aggccatcga agccaaggag cggatcgacg tcaccgtgcc
      601 gaccggccag gcggcgcgaa ttaccgtgca agatctgttt ctgcgataca agcacctcgc
      661 cggcatgacc ggcaccgccc gcacggcggc tcgcgaattc cgcaaggtct ataagaaagt
      721 cgtcgtgccg attccgacca atcgccgcgt gcagcgccag cgcctgtccg acaaggtctt
      781 tggcaccggc gaccagaaat ggcaggcgat cgtcgaagaa attcgcgagt tgaaccagct
      841 cggcaggccc gtgctcgtcg gcacgcggtc gatcgacaag tcggtgcacc tctcgcagct
      901 gctcgacgcc gtcggcatcc gccacaaggt ccttaacgct caccaggtcg ccgtggaggc
      961 cgaaatcgtc gcccaggccg gccgcgtcgg cggagtgacc atcgccacga acatggccgg
     1021 ccgcggcacc gacatcattc tcggcggcaa ctcgcaggag atggcctggg ccaagctgca
     1081 cgataaattc cccaccaagc tggacgttcc cgccgacgcc tggaagcagg ccgtcgaaga
     1141 aatcgagcaa gccgaaaagc tcgcagagga gcgggcgaag gttcgcgagc tgggcgggct
     1201 gcacgtcatt tgcaccgagc tgcacgactc ggcccgtatc gaccgccagc tcatcggccg
     1261 ctgtgggcgg caaggggatc cgggaaccgt ccgccaatac atgtcgctcg acgacgacgt
     1321 gatcctgacc ggcttcgggc cggaaacggc ccacaagatg gagctgctcg gccagcagaa
     1381 tggcgccagc gcccagcgct atctgggcct gctcaagcgc gcccagcgaa aagtcgagcg
     1441 ccgccacctc cgcgaccgct tcgtcctcct ctaccacgag aaggagcgga aaaagatgca
     1501 ggccgaaatg gggcaagatc cctatctcga cacgccggac tagaccagca gctcgtcgat
     1561 tggctgggcg gattccatgg tgaaccgcat cgggcggccg atcggcgtca gttcttcgtg
     1621 cgtgtaatcg acgccgagcg cttggagcac cgtggcgtgg atgtcttcca cgtttcgcgg
     1681 acccttcacg tcctgctggc ggttcttctc atcgagcttg ggctcggcag ccgtttcgcc
     1741 gatgactctg ccgcccttga tgccgccgcc ggccagggcc atcgtgaagc cgtgcggcca
     1801 gtggtcgcga ccgccgccat cgaggccgcg gattgtcgga gagcggccga attcgccgcc
     1861 ccacaggacg accgtcgaat cgaacagatc gcgctcctgg agctgcttga tcagcgtggc
     1921 aaaggcgggg tcgagaatgt cgcagcggcc cttgaccagc tcttcattgt tgacgtgcgt
     1981 atcccagccg ctgagcgtga cttcgacgca ccgtactcca acaccgacga gctgcacggc
     2041 tgccaggcac gcgcggccga agggcgtatc gccgaattct tgcttgaggc tctcgggcgc
     2101 ctcgcggaca tcaaacgcct tgagctgttc gctggtcatc atcttgcggg cggcggcgat
     2161 gctgacgctg tgcagggtct tttccggcag cgatttcact cggccttggg cgaacgcctt
     2221 ttcgaccacg ttctccaggt cgtccagccg gcgggccgct cggccggcag agaccggcgc
     2281 ggtgatatcg ggaatctgcc cgttgggatc gtaggtcttg aaggcgtcgt actgatcgcc
     2341 caaatatccg ccgcgggccg cccacgggcc gggaaggatc gaaacgtgcc gcggaatctc
     2401 gcacttgtcc tggccgagcg tctgatggca aaggatcgcc ccgagcgacg gatggaccag
     2461 cgtcggatcg ggccgccagc cggtcttaat gttgtacgcc gcccgctcgt gatcgccctc
     2521 cttgctgaag accgagcgga cgagcgagat gtgctccatc tgctcggcaa cttgcggcag
     2581 ccagtcggag atttcgatgt ccttggcagt cgttttgatg ttcttggtcg atgcgtgcga
     2641 gaacttcgtc ccagggtgcg ggtcgaacgt ctcaagctgg cttgggccac cttccatata
     2701 gagcacaatg agcgatttgg gaggcgcgcc cttgggcgcc tcttcct
//
LOCUS       DASUGB010000368         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_596206, whole genome shotgun sequence.
ACCESSION   DASUGB010000368 DASUGB010000000
VERSION     DASUGB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596206"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1200)
                     /locus_tag="VFV87_07080"
     CDS             complement(139..1200)
                     /locus_tag="VFV87_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103555.1"
                     /translation="MRSAHSRAFTLVELLVVIAIIGVLVALLLPAVQAAREAARRSDC
                     GNKLKQFGLALHNFHDVYLNLPPGAIDDDTNCMGWGVSILPYLEQKPLYDKFDGVFSQ
                     CVPNSGRPKLVMILKTGPHPGNSNVDSWGTGAQVDQPWRIDKALNQPLTKLSLPSFLC
                     PSNALPKFDNNGYGGSSYVGCMGNENWNSLTTRRVFVSTCGKPESIDQNGVLLHSNQN
                     NATECLNLAAILDGTSNTLMVGEIGQSLNVRPNVTNRENFPLWSGGNNGGGCKGWFIG
                     SHLRVADVITPINRKFNMATTPSSTPDLSDLGFGSFHPGGAQFVHADASVHFIPQTVN
                     TAIYRALGGRADGVSAQLP"
     gene            complement(1395..1856)
                     /locus_tag="VFV87_07085"
     CDS             complement(1395..1856)
                     /locus_tag="VFV87_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103556.1"
                     /translation="MHFHLRLRRPAAAAVLHLALGLVVGCGGSGPAKAPVKGKVLAGG
                     QPVTAGRLTFAPADGIDGAVPIVGQIQTDGTFVMSTDRPGDGIAIGKHLIAYTAPDAE
                     VTETENGADPIVKLSPYARLVVQQGEVEVKPGENDLIVELVPGAPPPAASE"
BASE COUNT          375 a          624 c          711 g          387 t
ORIGIN      
        1 ccccgcagcc gccggccgcc agggccagga tggtggttgc cgcgcacagc agcggccgcc
       61 tgtgaagagg ccaatgggcg ctttgattca tggtggaaag ctccttgcct aaggcaactg
      121 cgtctagggc aactgcgtct agggcaactg cgcggacact ccgtcggccc ggccccccag
      181 cgcccgatag atggccgtgt tgacggtctg cggaatgaaa tggacggagg cgtcggcatg
      241 gacgaactga gcgccgccgg ggtggaagct gccaaagccc aggtcggaga ggtccggagt
      301 gctgctgggg gtcgtggcca tgttgaactt gcgattgatc ggcgtaatca cgtcggccac
      361 ccgcaggtgc gagccgatga accagccttt gcagccgccg ccgttattgc cgcccgacca
      421 gagcggaaaa ttctcgcggt tggtgacgtt cggcctgacg ttcaggctct ggccgatttc
      481 gcccaccatc agcgtgttgc tggtgccgtc caggatggcc gccaggttga ggcactcggt
      541 ggcgttgttc tggttgctgt gcaggagcac gccgttctgg tcgatcgatt cgggcttgcc
      601 gcacgtgctg acaaagaccc ggcgggtggt caggctgttc cagttttcgt tgcccatgca
      661 gccgacgtag ctcgagccgc cgtagccgtt gttgtcgaac ttcggcagcg cattcgaggg
      721 acagaggaac gaggggagcg agagctttgt caggggctgg ttcagcgcct tgtcgatgcg
      781 ccagggctga tcgacctgcg cgccggtgcc ccagctatcg acgttggaat tgccggggtg
      841 cggccccgtc ttgaggatca tgacgagctt cggtcggccg gaattcggca cgcactgaga
      901 aaacacgccg tcgaacttgt cgtagagcgg cttttgctcg agatagggga ggatgctcac
      961 gccccagccc atgcagttgg tgtcgtcgtc gatggctccg ggaggaaggt tgagatagac
     1021 gtcgtgaaaa ttgtgcagcg ccaggccgaa ctgcttgagc ttgttgccgc agtcgctgcg
     1081 gcgggcggcc tcgcgggcgg cctgcacggc gggcaagagc aacgcgacca ggacgccgat
     1141 gatggcgatg accaccagga gctcaacgag ggtgaacgct cgcgaatgag cactcctcac
     1201 gatgtttcgt gtgcgactta ccatgaatta cccccaggaa acggaagttc gtcggattat
     1261 ggcaatggag cgaacgctcc gccagaatgg acgaatcgta cacgaagttc ctgtggcaat
     1321 tcaagtgttc gtatgcgaat tttccaggat tcttgggttg gcacttcggc ggcgtctgtg
     1381 ccgttttggg acccttactc cgaagcggcc gggggcggcg ccccggggac cagctcgaca
     1441 atcagatcgt tctcgccggg cttcacctcg acctcgccct gctgcacgac cagccgcgca
     1501 tagggcgaga gcttcacgat cggatccgca ccgttttccg tctcggttac ctcggcatcc
     1561 ggcgccgtat aggcgatgag gtgtttgccg attgcgattc catcgcccgg ccgatcggta
     1621 ctcatcacga acgtgccgtc cgtttggatt tgcccgacga tagggacggc cccgtcaatt
     1681 ccgtcggccg gggcaaaggt cagccgcccc gccgtgaccg gctggccgcc ggccagcacc
     1741 ttgcctttga ccggcgcctt cgccgggccg ctgccgccgc agccgacgac cagacccaat
     1801 gcaagatgca gaacggccgc cgccgcaggg cgccgcaatc gcaggtggaa gtgcatgaca
     1861 gttcccttga aaaggagatt gagccggaaa ggcgaaaaga tggccggcag cgcagccgct
     1921 gggcggaaaa aaagggacca gtgcccgctc gtgcggcgtg cccgacctaa ggcagttgca
     1981 cggggacatt gtcgttgcgc ccgcccatcg ccatatacac gtttacgttg accgtgttgg
     2041 gaatgaagcg gacggaggcg tcagcgagcg tgaactgcgc gccgccgggg tggaagc
//
LOCUS       DASUGB010000369         8135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_180571, whole genome shotgun sequence.
ACCESSION   DASUGB010000369 DASUGB010000000
VERSION     DASUGB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180571"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFV87_07090"
     CDS             <1..285
                     /locus_tag="VFV87_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103557.1"
                     /translation="RRGYGSRGGDSSSGEGSSAELPQLANYRITPATERLPKDLPSWF
                     LRGDVDGDGQVSMAEYTTTWSDQLAAEFIKLDANGDGIITPAECLAAAAR"
     gene            complement(303..1787)
                     /locus_tag="VFV87_07095"
     CDS             complement(303..1787)
                     /locus_tag="VFV87_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103558.1"
                     /translation="MTAASHLKTLLAESRLMNRMLNPAGRNMPCPGPLSRRSLLSAGA
                     AAFTGLGMADLLRLQAQAGDSFQNGKSNDPAVIFVWLPGGPPHMETFDMKPDAPEDYR
                     GIFRPINTNVSGIQICEHLPLLAKLADKYTLIRSIAHGFADHGGGHKRFMTGRDPKEP
                     TGFVNDYPAVGSMVAKMQERRNVGVPNYALLVDSGRANIDTFSLGAAYLGPAYGPFTV
                     GGDPSEPEFKVQNLGLAKEMEERLDDRATLLGGLDKLRRGIDKSGALDAMDKFNQRAV
                     DLLTSEKARAAFDLTQEDPKLREKYGDHPWGQRAIMARRLVEAGVSFVTVVMENPFCK
                     QVPWIKNGVYNWDSHAVNCHLFDDALVRLPVFDRTIAAMIEDIYARGLDKRVMVVVTG
                     EFGRTPRITNQIGTQTGIMQPGRDHWPGAMSVLAFGGGMRTGQVVGATNSKGEYPSDR
                     PLTPNDLWATVFQHLGINTHHAFPDLSGRPMPILPFGEPIRELA"
     gene            1972..3204
                     /locus_tag="VFV87_07100"
     CDS             1972..3204
                     /locus_tag="VFV87_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5103559.1"
                     /translation="MKSGIGKVLGGGRLFGAEMRDDPYPIYRELRESDPVHWDDSLHA
                     WILTRYDDVGLALNDPRFSSRRVSRARQRFADPSLAPLFDTLAGRMSDHDEPDHKRLR
                     ALVHDAFLRTAVEKWTPRIQARIDLLLAAAEQRGTIDFIADFAVPLPLLVILEIVGVP
                     ATDRVRVKAWCDDFAIVALNFYANISEAELHNGLRSIVEFRQYLGERIGELESSPRTD
                     LLSSLVQVEHEGTRLTLDELLANALLLLAAGNETTTCVLGNGLAALLNHPEQLELLRR
                     NPSLIPAAVEEFLRYDSPVQFLGRIALEDVELRGQTIRRGDLVLAVLGAANRDPARFA
                     EPDRLDVTRAHVPHFAFGHGPHFCAGAQLARLEAQMAFAGLLARFRAIELDTTSPLVR
                     RENFNIRCWEQLPVRLNV"
     gene            3817..4056
                     /locus_tag="VFV87_07105"
     CDS             3817..4056
                     /locus_tag="VFV87_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103560.1"
                     /translation="MKFSVRDMLWMMVVVGLTVGWWTDHRHQAQTKLKFTVVERMLQH
                     MLNQYYADTGRYMEVGEDSLGLDYHPTNVQTATGR"
     gene            complement(4120..5181)
                     /locus_tag="VFV87_07110"
     CDS             complement(4120..5181)
                     /locus_tag="VFV87_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX5103561.1"
                     /translation="MRSCAIIALWIAACGYSPDASSLAAEPQTVKVFILAGQSNMEGK
                     APNALLNHQATDPKTKDLFSHLRKDDAWIVRDDVFIKFLERKGGLTIGFGSPDRTGVE
                     LEFGAVMGDHFDEPVLLIKTAWGGHSLVKNFRSPSAGLPSEEQLQAELEQARQRIQKN
                     NEKNKRKEPLPEMADIKEGYGASYRMMLAEVQGVQENYESLFPELKGKKLELAGFVWF
                     QGWNDQYGGQDEYASNLKHFIQDVRKDLNAPKLPLVIAAMGQNGSKPAQGAMLTVQQA
                     QLSMNDVPEFQGNVRAIRTDELADKAAEELYPNWQDHFEEWKLVGGDRAYHYLGSAIW
                     FNRIGKAMGEAMLEIMDKP"
     gene            complement(5231..6616)
                     /locus_tag="VFV87_07115"
     CDS             complement(5231..6616)
                     /locus_tag="VFV87_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103562.1"
                     /translation="MNRRQWLSIGGISLGALASGGLPGLAGLLAAEEASREVSRDFSV
                     ILFWANGGPSHIDLFDLKPQAPLEYRGPFRPIRTNVPGIEICELFPQLAKLADRFALL
                     RSLHHERAEHSGGSHRFLTGYASKAANLPVSEYPDIGSIVAHHFANRESPVPPFVANT
                     RSYGCGPGYLGPAYAAYMPSPNPITASGENEYDPVPIYQTAGTQNDLSISSGGVMSLR
                     RREDLLKTLDRLPRELDASGSLSALDSFQQRAVSMLASERARAAFDLSLESEKTRERY
                     GDTHWGRSLLTCRRLVEAGVRFVQCQAEFRLRPETGVTSSWDDHAVNCHIFKALEEKL
                     PVLDQSLSALVEDLHARDLDRHVLVLFCGEFGRTPRISYGKGADRPGRDHWPRAMSVF
                     AAGGGLKMGQAIGATNSRAEEPVEQAMNSNCLLATLYRRFGIDTRTMLTDNSGRPLPI
                     LPSGEPIRELI"
     gene            6727..7569
                     /locus_tag="VFV87_07120"
     CDS             6727..7569
                     /locus_tag="VFV87_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5103563.1"
                     /translation="MQQALFVVACLLSIAASPALAEPAKLIVKKDVVYGHVQGAGLLA
                     DIAYPAGAEKLPVILSVHGGRWVGSHRNDPGEGAIDVEKWAAHGLFAMTIDYRLVGCT
                     PPPACYQDVQCAIRWVHAHAGEYPIDTERIFLIGMSAGGHLVSLAATLGDGPFPRTGG
                     WEKASNEIRGAISLSGPYDLEKLSWGKLWTPSSTGAAAAQRLASPILHVSEKSKPLLI
                     LHSDNDGSVPIQQALDMATALEKAKTPHRIRIFEKQGHMRITEEVIKESLAFIQEQSR
                     SAHE"
     gene            7562..>8135
                     /locus_tag="VFV87_07125"
     CDS             7562..>8135
                     /locus_tag="VFV87_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5103564.1"
                     /translation="MSDKEPLSWFGALRRNDVPAIRKWIAEGADIDAPIPLKPTDSKW
                     PPLYVAALGGKSEGVRVLLEAGADPNVLQALPDQPTEGYTYRFDSPLIAAAGHGSAEA
                     VRLLLDAGADIAYCGPISDSALAEAIHLADGKKFLDVIRLLLDRGCAADYTPTGRSLL
                     ISAAWNRNCRGIISELVKRGADPNAIDQRWG"
BASE COUNT         1441 a         2655 c         2558 g         1481 t
ORIGIN      
        1 cggcgcggct atggctcgcg cggcggcgat tcgtccagtg gcgagggaag cagcgccgaa
       61 ctcccgcaac tggcgaatta ccgcatcacg cctgccaccg agcgcttgcc caaggacctg
      121 ccgagctggt tcctccgcgg cgacgtcgat ggcgacggcc aggtttcgat ggccgaatac
      181 acgaccacct ggtccgacca gctcgccgct gagttcatca agctcgacgc caacggcgac
      241 ggcatcatca cccccgccga atgcctggct gctgctgcca ggtagctccg ccagcgcctg
      301 aactacgcca attcccgaat cggctcgccg aagggcaaga tcggcatcgg tcgcccgctg
      361 aggtcgggga aggcgtggtg ggtgttgatc cccaggtgct ggaagaccgt ggcccacagg
      421 tcgttgggcg tcagcgggcg gtcggagggg tattcgccct tgctgttcgt cgcgccgacg
      481 acttgcccgg tccgcatccc gccgccgaag gccagcacgc tcatcgcacc gggccagtgg
      541 tcgcggcccg gctgcatgat gcccgtctgc gtgccgatct ggttggtgat ccgcggcgtg
      601 cggccaaatt cgccggtgac gacgaccatc acccgcttgt cgaggccgcg ggcgtagatg
      661 tcttcaatca tcgccgcgat cgtgcgatcg aagacgggca gccgcacgag ggcgtcgtcg
      721 aacaggtggc aattgaccgc gtgcgagtcc cagttataga cgccgttctt gatccacggc
      781 acctgcttgc agaacgggtt ttccatcacg acggtgacga agctcacgcc cgcctcaacc
      841 agccgccggg ccatgatcgc ccgctggccc cagggatgat cgccgtattt ctcgcggagc
      901 ttcgggtcct cttgcgtcag gtcgaaagct gcccgcgctt tctcgctcgt tagcaggtcc
      961 actgcccgct ggttgaactt atccatcgcg tcgagcgcgc cgctcttgtc gatgccgcgg
     1021 cggagcttgt cgagcccgcc caagagcgtg gcccggtcat ccagccgctc ttccatttcc
     1081 ttggccaagc ccaggttctg caccttgaat tccggctcgc tcggatcgcc gccgacggtg
     1141 aacggcccat aagccggacc gagataggcg gcgccgaggc tgaatgtgtc gatgttcgcc
     1201 cggccgctat cgactagcag cgcgtagttg ggcacgccga cgttccgtcg ctcctgcatc
     1261 ttggcgacca tcgacccgac cgccggatag tcgttcacaa agccggtcgg ctccttgggg
     1321 tcgcggccgg tcatgaaccg cttgtggccg ccgccgtgat cggcaaagcc gtgggcgatc
     1381 gagcggatca gcgtgtattt gtcggccagc ttcgccagca gcggcaaatg ctcgcaaatc
     1441 tggatgccgg agacgttcgt gttgatcggc cgaaagatgc cgcggtaatc ctccggggcg
     1501 tccggcttca tgtcgaacgt ttccatgtgc ggcggcccgc cggggagcca gacgaagatc
     1561 acggcggggt cgttcgattt gccgttttgg aacgagtcac cggcctgggc ctgcaagcgc
     1621 agcaaatcgg ccattccgag cccggtaaat gcagccgccc cagccgatag gagcgaccgg
     1681 cgcgagaggg gaccagggca gggcatattc cgaccggcag gattgagcat ccgattcatc
     1741 aggcgggact ccgcgaggag cgtcttcagg tgggaagcgg ctgtcattca ccgctgcccc
     1801 tattttggcc ctaaaggggc atccatgtca atttgcttat cgatcggccg cagccggagc
     1861 gtgagacttc ggtgagtttt ggagtcctgt gaaccgaagt cttacgagtt cagctaccgt
     1921 gttagcatac gcgtacgaaa cctagccacg tgctttcgct tgggagacgc agtgaaatcg
     1981 gggatcggca aagtgctcgg cggcggtcgc ctgtttgggg cggagatgcg cgacgatccg
     2041 taccccatct atcgcgagct gcgcgagagc gacccggtgc attgggacga ttcgctgcac
     2101 gcctggatcc tgacgcggta cgacgacgtc ggcctggccc tcaacgaccc gcggttttcg
     2161 tcccgccgcg tgagccgcgc ccgccagcgg tttgccgatc cgtcactggc cccgctgttt
     2221 gacacgctgg ccgggcggat gtcggaccac gacgagccgg accacaaacg cctgcgggcc
     2281 ctcgtccacg acgcgttcct gcgcaccgcc gtcgaaaagt ggacgccgcg gattcaggcc
     2341 cgaatcgacc tgctcctcgc cgcagccgag cagcgcggca cgatcgactt catcgccgat
     2401 ttcgcggttc cgctgccgct gcttgtgatt ctggagatcg tgggcgttcc cgcgacggac
     2461 cgcgtgcggg tcaaagcctg gtgcgacgat ttcgcgatcg tggcgctcaa cttctatgcc
     2521 aacatttccg aggccgagct gcacaacggc ctgcggagca tcgtcgaatt ccgccagtac
     2581 cttggcgagc ggatcggcga gctggagagt tcgccgcgga ccgaccttct cagctcgctg
     2641 gtgcaggtcg agcacgaagg gacccgcctg acgctcgacg agctgctcgc caacgctctc
     2701 ttactgctgg ccgccggcaa cgaaacgacg acctgcgtac tgggcaacgg cctggcggcg
     2761 ctgctcaacc atccggagca gttggaattg ctgcggcgca atccttccct catccctgcc
     2821 gcggtcgaag agttcctgcg gtacgacagc cccgtgcagt tcctggggcg gatcgcgctc
     2881 gaggacgtcg agctccgcgg ccagacaatc cgccggggcg atctcgtcct cgccgtgctc
     2941 ggcgcggcca atcgcgaccc agcgcggttc gccgaaccgg atcggctgga tgtcacgcgg
     3001 gcgcacgtgc cgcattttgc cttcggtcac gggccacact tttgcgccgg ggcgcaactc
     3061 gcccggctgg aggcgcagat ggccttcgcg gggctgctcg cgcggttcag agcgatcgag
     3121 ctcgacacta cctcgcccct cgtccgccgc gaaaacttca acatccgctg ctgggagcaa
     3181 ctgcccgtgc ggctgaatgt ctgacaggca gtccgagcta gcccgagaca tcaagaatcc
     3241 aacgcgggac tgccagcgcc acgcaaacgg acatcgcaac caggagcatc agccagaaaa
     3301 gtgtgatcag ggaaaaccgg cgacggccaa ttgcgaacgc gacaaatccc acgataactg
     3361 tcacgggaag cgatgcacag actgccgcga tgattagacg agtgtcccac atttcggcat
     3421 tctcatagcc aaatgcgacc gccaaaaggc tgctgggccg gatggttcat ccgaaggatg
     3481 cgtgcgccgg gagctcccgg ccgcctccct tggattttag aggcatggct ccggtgtcca
     3541 atggctgttg gacacggatt gccggatcgg ctatcgttgc tttgcccggc gaaacggagt
     3601 caggctctgc tgggaagcaa cctgacaagg gataaccggg ccgacggtgc gccaatggtg
     3661 cagccggccc gtggcagcaa ctgttggcag gggccgcttt cgtaaatcca ttggacaccg
     3721 gagcgatgcc ttagaaaatc ccgcctgcgg cgtcaggatg acccaccgac ttactgtcgc
     3781 gcacgctaca gccagatgaa ccggtagaat ctaggaatga aattctccgt ccgcgacatg
     3841 ttgtggatga tggtcgtcgt gggactgacc gtggggtggt ggaccgacca tcggcaccaa
     3901 gcgcaaacga aactcaaatt caccgtcgtc gagcgaatgc tccagcatat gctgaaccaa
     3961 tactacgcag acacgggacg ctacatggag gtgggagaag attcactcgg cctcgattac
     4021 cacccgacga acgtacagac cgccaccggc cgctagacgc ggctgaacgt gtgggacacg
     4081 tttttaacct gtccaaacgg acgggttggg aaacccgtcc tacggcttgt ccatgatctc
     4141 cagcatcgcc tcgcccatcg ctttgccgat gcggttgaac cagatggcgc tgcccaggta
     4201 gtgatacgcc cggtcgccgc cgaccagctt ccactcctcg aagtgatcct gccagttcgg
     4261 atagagctcc tcggcggctt tgtcggccag ctcgtcggtg cggatcgccc gcacgttccc
     4321 ctggaactcc ggcacgtcgt tcatcgaaag ctgggcctgc tgcaccgtca gcatcgcccc
     4381 ttgggcgggc ttggagccgt tctgccccat cgccgcgatg accagcggca gctttggcgc
     4441 gttcagatcc ttacggacat cctgaatgaa gtgcttcagg ttggacgcat actcgtcctg
     4501 cccgccgtac tgatcgttcc acccctggaa ccagacaaac ccggccagct ccagcttctt
     4561 gcccttcaac tccgggaaga gcgattcgta gttttcctgc acaccttgca cctcggccag
     4621 catcatccgg tacgacgcgc cgtagccctc cttgatgtcg gccatttccg gcagcggctc
     4681 tttgcgcttg ttcttttcgt tgttcttttg gatccgctgc cgggcctgct ccagctccgc
     4741 ctgcagctgc tcctcgctgg gcagcccggc cgatggcgag cggaagtttt tcaccagcga
     4801 atgcccgccc caggcggtct tgatcagcag caccggttcg tcgaaatggt cgcccatgac
     4861 ggcgccgaac tccagttcca cgcccgtccg gtcaggcgac ccaaagccga tcgtcaggcc
     4921 gcccttgcgc tccaggaact tgatgaagac gtcgtcgcgg acgatccacg catcgtcctt
     4981 gcgcaagtgc gaaaagagat ccttcgtttt cggatcggtc gcctggtggt tgagcaaagc
     5041 gttcggcgcc ttgccttcca tgttcgactg gcccgccagg atgaagacct tgacggtctg
     5101 cggctcggcg gcgagcgacg aggcgtccgg cgaatatccg caagccgcaa tccacaacgc
     5161 gataatggcg caagagcgca ttggtactcc tcgatgaagg cctggttgcg tcgccgatcc
     5221 acactgtccg ctagatcaat tcccggatcg gctcgccgga gggcaaaatc ggcagcggac
     5281 ggccactatt gtcggtcagc atcgtgcggg tgtcgatccc gaagcggcga tagagcgtcg
     5341 ccaacaggca gttgctgttc atcgcttgct cgaccggctc ttcggcccgc gaattggtcg
     5401 cgccgatcgc ctgacccatc ttcagcccgc cgcccgcggc aaagacgctc atcgcccgcg
     5461 gccagtggtc gcggccgggg cggtcggcgc ccttgccata gctgattcgc ggcgtgcggc
     5521 cgaattcgcc gcagaagaga accagcacgt gccggtccag atcgcgggcg tgcaggtcct
     5581 cgaccagtgc cgagagcgac tgatcgagca ccggcagctt ctcttcgagg gccttaaaga
     5641 tgtggcaatt gaccgcgtga tcgtcccagc tactcgtgac gcccgtctcg ggccgcaggc
     5701 ggaattcggc ctggcattgc acaaaccgga cccccgcctc caccagccgt cggcaggtga
     5761 gcaggctgcg cccccagtgt gtatcgccgt agcgctcgcg cgtcttttca ctctctagag
     5821 acaaatcgaa cgcggcccgc gcccgctcgc tggccagcat cgacacggcc cgctgctgaa
     5881 aggaatcgag ggccgagagg ctgccgctgg catccagctc ccgcggcagg cggtcgagcg
     5941 ttttcagcaa atcctcgcgg cggcggagcg acatcacgcc gccggacgag atcgacaggt
     6001 cgttctgcgt gcctgctgtc tggtagatcg ggacggggtc gtattcgttc tcgcccgaag
     6061 ccgtgatcgg attggggctg ggcatatatg ccgcgtacgc cggccccagg tatcccggcc
     6121 cgcagccgta cgaacgcgta ttggcaacga acggcggaac ggggctctcg cgattcgcaa
     6181 agtgatgggc gacgatcgag ccgatgtccg gatactccga gacgggcaga ttcgccgcct
     6241 tcgacgcata gccggtcaga aaccggtgcg agccgccgga atgctccgcc cgctcatggt
     6301 gcaggctgcg gagcagcgcg aaccgatcgg ccagcttggc aagctgcgga aacagctcgc
     6361 aaatctcgat ccccggcacg ttcgtccgga tcggccggaa ggggccgcga tactccagcg
     6421 gcgcctgcgg cttcaggtcg aacaggtcga tgtggctcgg gccgccgttg gcccagaaca
     6481 gaattaccga gaaatcgcgg ctgacctcgc gcgaagcctc ttccgccgcc agcagccccg
     6541 ccagccccgg caatccgccg ctcgccaaag ccccgaggct gatgccgcca atcgacagcc
     6601 actgccgtcg attcaacggc ccggcgcatt gggttgttgg ttttggcatg actggccccg
     6661 ctatggccgg tggattatat caatgggggc tgatggttgt cacgggtttt ttggcggagt
     6721 ccgaagatgc agcaggcact attcgtcgtc gcgtgtctct tatcgattgc tgcttcaccg
     6781 gcgcttgccg agcccgcgaa gttgatcgtc aaaaaggacg tcgtctatgg ccacgtgcaa
     6841 ggggcggggc tgctcgccga tatcgcctat cccgcagggg ccgagaagct gccggtgatt
     6901 ctgtcggtcc acggcgggcg ctgggtaggc agccatcgca acgatccggg cgagggggcg
     6961 atcgacgtcg agaaatgggc ggcccatggc ctgttcgcga tgacgatcga ttaccgcctg
     7021 gtgggctgca ctccgccgcc cgcttgttac caggatgtgc agtgcgcgat ccgctgggtg
     7081 catgctcacg ccggcgaata cccgatcgac acggagcgaa tcttcctgat cggcatgtca
     7141 gccggcgggc acctggtttc gctcgcggcg acgctggggg acgggccctt cccgcgcacc
     7201 ggcggctggg aaaaagcgtc gaacgaaatt cgcggagcga tcagcctctc gggcccctac
     7261 gacctggaaa agctctcctg gggcaagctt tggacgccct ccagcacggg cgctgccgcg
     7321 gcccagcgcc tggcctcgcc gattctgcac gtttccgaga aatccaaacc gctattgatt
     7381 ctgcattccg acaacgacgg ctcggtgccg attcagcagg ccctcgacat ggcgaccgcg
     7441 ctcgaaaagg ccaaaacgcc gcaccgaatc cgcatcttcg agaaacaagg gcacatgcgg
     7501 attacggagg aggtgatcaa ggaatcgctc gcttttattc aagagcaatc aaggagcgcc
     7561 catgagtgac aaagagccgc tttcctggtt tggcgcactc cgacggaatg atgtgcccgc
     7621 gatcaggaag tggatcgccg aaggcgccga catcgacgct ccgatcccgt tgaaacctac
     7681 tgattcaaag tggccgccgc tctacgttgc cgcgctcggc ggcaagtccg aaggcgtgcg
     7741 cgtcttgttg gaggcgggcg ctgacccgaa tgttcttcag gcgctgcctg accaaccgac
     7801 cgaaggctac acgtaccgtt tcgactcgcc cctgattgcc gccgcgggcc acggcagcgc
     7861 ggaagcggtc aggctgcttc tggatgccgg cgcggatatc gcgtattgcg gaccaatcag
     7921 cgacagcgcc ctggccgaag ccatccattt ggcggacgga aagaagtttc tggacgtgat
     7981 ccggctgctc ctcgaccggg gctgcgctgc ggactatacg cccaccggcc gatcgctcct
     8041 gatctccgcc gcttggaacc gcaattgccg cggaatcatt tccgagctcg tcaagcgcgg
     8101 cgccgatccg aacgcgatcg accagcgctg gggct
//
LOCUS       DASUGB010000370         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_596257, whole genome shotgun sequence.
ACCESSION   DASUGB010000370 DASUGB010000000
VERSION     DASUGB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596257"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFV87_07130"
     CDS             complement(<1..489)
                     /locus_tag="VFV87_07130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HEX5103565.1"
                     /translation="MAQLDRTIIENIVRQIVLAQKGGATPTRSVSEGEGAYTPNLVCS
                     ISARHCHLTDEHVEVLFGPGAKLIPEKNLYQDGFYAAEQTVMVVGPRRRMLPTVRVLG
                     PTRPASQVELAFTDSISLGIDAPVRHSGNIRGTPGCVLVGPKGVVELQEGVIRAARHV
                     HMS"
     gene            complement(520..1341)
                     /locus_tag="VFV87_07135"
     CDS             complement(520..1341)
                     /locus_tag="VFV87_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX5103566.1"
                     /translation="MIKLASTDDRRTRLIELIRQRGFASLPALAEQLAVSESTVRRDL
                     DFLEESGVAQRTHGGVFYTGPSPKLAHFDQRQSLNWDKKRQIAVAASRLIDDNDTILL
                     DGGSTTYELAQLLVGRPLQVVTNSLPVANLFTSSESADLVIVGGYVHAKTGVSLGPYA
                     NQMLAGLNVRRAVLSVAGATERGYFNSNLLLVETERAMMNAADEVIVVADSTKFGHTS
                     LAHLSDLDSIDILVTDHEIDPAWREKLAAAGVKVIVAEAAPVGQASSLPDEEQAK"
     gene            complement(1491..2090)
                     /gene="purN"
                     /locus_tag="VFV87_07140"
     CDS             complement(1491..2090)
                     /gene="purN"
                     /locus_tag="VFV87_07140"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5103567.1"
                     /translation="MSAAPLPIAVLISGGGTTLRNLIERIAAGSLPVEIRLVISSGPA
                     ARGLEYAREASIPALVVEKKKATSDDEFCRAIFQPCRAAGVKFVVMGGFLKHVLIPPD
                     FENRVVNIHPALLPAFGGKGMYGLKVHQAVIDAGATITGCTVHFVDNEYDHGPILLQR
                     EVEVLPGDTAESLQARVFLAERELYPEALRVLAAGESSE"
     gene            complement(2176..4342)
                     /locus_tag="VFV87_07145"
                     /pseudo
     CDS             complement(2176..4342)
                     /locus_tag="VFV87_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
BASE COUNT          757 a         1319 c         1388 g          878 t
ORIGIN      
        1 gctcatgtga acgtggcggg cggcgcggat cacgccttcc tgcaattcga cgactccttt
       61 ggggccgacc agcacgcagc cgggagtgcc gcggatattg ccgctgtggc ggacgggggc
      121 gtcgatgccg agcgaaatgg aatcggtgaa tgccagctcg acctggctgg ccggccgcgt
      181 cgggccgagg acgcggaccg tcggcagcat ccggcggcgc gggccgacga ccatcaccgt
      241 ctgctccgcc gcgtaaaaac cgtcctggta caggttcttt tccgggatga gcttcgcgcc
      301 ggggccgaag aggacttcga cgtgctcgtc ggtcaggtgg cagtggcgag ccgagatgct
      361 gcagacgagg ttcggggtgt aagcgccctc gccctcgctt acgcttcggg ttggtgtcgc
      421 gccgcctttt tgggccagca cgatttgccg gacgatgttc tcgatgatcg tgcggtcgag
      481 ttgggccatg atattcgtgg ggtaggcgtc tcgtctgcct tacttggctt gttcttcatc
      541 tggcaggctg gaagcctgcc ccacgggcgc tgcttcggcg acgatcacct tcacccccgc
      601 ggcagcgagc ttctcgcgcc aggcgggatc gatttcgtgg tcggtcacca ggatgtcgat
      661 cgaatcgagg tcggacaggt gggcgaggct cgtgtggccg aacttggtgc tgtcggcgac
      721 aacaattacc tcgtcggcgg cgttcatcat ggcccgctcg gtttcgacga gcagcaggtt
      781 actgttgaag tagccccgct cggtcgcgcc ggccacactg agcaccgccc ggcggacgtt
      841 caggcccgcg agcatctggt tggcgtaggg ccccagcgat acgccggtct tcgcgtggac
      901 gtagccgccc acgatcacca ggtccgccga ttcgctggag gtaaacaggt tggcgacggg
      961 caacgagttg gtcaccactt gcagcgggcg gccgaccaga agctgagcca actcgtaggt
     1021 cgtgctcccg ccgtccagca ggatcgtgtc gttgtcgtcg atgagccgac tggcggcgac
     1081 cgcgatctgc cgcttcttat cccaattcag cgactgccgc tggtcgaaat gggccagctt
     1141 gggcgacggg ccggtgtaaa acaccccgcc gtgcgtccgc tgggccaccc ccgactcttc
     1201 gaggaaatct aggtcacgcc gaaccgttga ttcggatact gccaactgct ccgcgagcgc
     1261 cggcagcgac gcgaaacccc gctggcggat cagttcgatc agccgggttc gcctgtcgtc
     1321 ggtgcttgcc aacttgatca ccgcctgcac actccacgca acttgcgcca attatgacgt
     1381 gtttcttgca atttgcaatc atgaagtgat agaaatctat cagattgtgc gcgtaaatac
     1441 tctcacagcc gtcgcaatga tcgcaagtgc ttgtgcagta atgagttgcg ctactcggac
     1501 gactcgccgg ctgcaagcac ccggagggct tcgggataca gttcccgctc ggccaggaag
     1561 acacgggctt ggagcgactc agcagtgtcg cccggcagca cttcgacctc gcgctgcagc
     1621 aggatcggcc cgtggtcgta ctcgttatcc acgaaatgca ccgtgcagcc ggtgatcgtc
     1681 gcgcccgcgt cgatcaccgc ctgatggacc ttcagaccgt acatcccctt gccgccaaac
     1741 gccggcaaaa gagccggatg aatgttgacg acgcgattct cgaaatcggg tggaatgagc
     1801 acatgcttca gaaagccgcc catcacgacg aacttcacgc ccgccgcccg gcagggttga
     1861 aagattgccc ggcagaactc gtcgtcgctc gttgctttct tcttctcgac gaccagcgcc
     1921 ggaatcgaag cctcccgcgc gtattccagc ccgcgcgccg ccgggccgct cgaaatcacc
     1981 agccgaatct ccaccggcag cgagcccgct gcgatcctct cgatcaagtt gcgcagcgtg
     2041 gtgccgcctc cggagatgag gacggcgatg ggaagtgggg cggcggacat cggcatgttt
     2101 ggccgcggat tgcacggatg gcgcggataa gattcctatc cgtgtagtcc gcgccaatcc
     2161 gcggctcatc tctcgctaaa tgcgtcgcac ataaagaacg atcttctcgt cgccgatctc
     2221 ggcttcgatg ccgtccactc ctggcaatgc ctcaaacaca agcttgacgg caagcgtttc
     2281 gccacaaaca tagtcgcgaa agccctcgat cgctttttgc aatgcgggcg aagcgccgac
     2341 aatgccaacc tcgatctggt cagcaaaatt gaggcttaaa tctttgcggc ggtcttgaac
     2401 caggcggacg aagtcccggg cgaggccttc gccgatcaat tctggcgtta gttccgtcgc
     2461 taacacaacg acgcatcccg ctccctgagc ggcggcccaa ccgggcttcg cttggagtcg
     2521 gacctggatg tcctcgttgt cgagttcgac cttgtcgccg ccgaggtcga gcgtgacttt
     2581 gccggtcgcg gtcaattccg ccagcagctt gccgccgtcg gcattcccga gcgaagcctt
     2641 gcacgccggc aggagcttgc cgatccgcgg gccgagccgc ttgaagttcg gctggacctg
     2701 gtaggtgatg tatttctcgg cgtccttggt gtactcgatc gttttgacgt tcagctcgtc
     2761 gcgcaaaagc tcgtcgtgct cttcgagcca cgcctggtgc gcctggtcgg aaaggatcac
     2821 ttcgaccttc gccagcggct ggcgaacctt cagcttgttc gccatgcgag ccgagaggcc
     2881 gagcgaggcg atctgacgga gcaggtccat gcgctcggag agcgtggcat cgatcttggc
     2941 ggaatcagcg gttgggtaat cgcagaggtg gacggatgcc gctaccggaa cgccacggag
     3001 gacgttccct acagcgctga atgatcccgc cagattccgc cacattgcct cggcgacaaa
     3061 cggcgtgaag ggggcgatga gcttggcggt ggtcagcagg gtttcgtaga gcgtccagta
     3121 ggcgtccagc ttgtcctgct ggtgctgggc gtccattgcg gcccaaaagc ggtcgcggct
     3181 acggcggacg taccagttgg agaggccatc gacgaacgcg tgcaagcgct tgcaggcttc
     3241 gtagttgtcg taggcgtcca tccgctcgac gacggattgg attgtgcggt tcagctcgct
     3301 caggatccag cggtcgagtt cgccgcgctg gttagcgggg cggtagccat ccgccttcgc
     3361 cagttcagcc ggcgtcagtt ggaacgcgga cgcgcttccc ttcggggcgc ggttaaacga
     3421 gcgttccggc tcgaatttgt cgttcgagtt cgcgtagttg acgaagaacg agaaacaatt
     3481 ccagagccgg aggatgaact cggggatggt ttccttgatg gctcggtcgc tatagatcgt
     3541 tccggtccat ggcggctgcg cggcaaagaa ataccagcgc aatgcgtcgg cgccgaattt
     3601 gtcaaagatt tcaaacggat tgctgtagtt tcgcagtcgt ttagacatct tgccgacttt
     3661 ccgcacggaa tctttgccaa gggcggcgcg ggcttcgtcc tcactagcga acaactgctt
     3721 tccgtctttg ctttcccacc attcagtgag catcaggccg aggacgatgc agttcttgaa
     3781 cggatgtggg tagggagttg cgggcaattg cggattgcgg attgcggatt gcggatcggc
     3841 ggcggctgtc gactgcttag tactaagtac tgagtactga gtactgggct gcgaggcgcc
     3901 tccccctgtc ccctgtcccc tgtccaaaca gcaacgtgct gatggcaagc tggctataga
     3961 accagcctcg cgtctgatcc aacgcctcgc tgatgaagtc ggccggaaac tgctgcgaga
     4021 acttctcgac gcccgcgtgc ggatagcccc attgggcgaa gggcatggcg ccgctgtcgt
     4081 accagcagtc gatgacttcg gggacgcggc gcatgcgtgc gccggcggcg tgcggcgagt
     4141 cgtaggtgat cgcgtcgatg tagggcttgt ggactttcag gtcatcgacg agcgacggat
     4201 tggccttttt cgcggcggcc cagacttcct ggccggtgac gccgggcttg ttcagcagct
     4261 cgtcgtagct ggcgacggcc tccatctggc cggtctgctc gcagacccag atcggcagcg
     4321 gcgtgcccca gtagcgctcg cg
//
LOCUS       DASUGB010000371         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1709084, whole genome shotgun sequence.
ACCESSION   DASUGB010000371 DASUGB010000000
VERSION     DASUGB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709084"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFV87_07150"
     CDS             <1..560
                     /locus_tag="VFV87_07150"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5103568.1"
                     /translation="MRPKYEMEALSLHEAVPGHHLQLALQAELGELPPFRRYGGFTAY
                     IEGWGLYAESLGDQLGCYQDPYSKFGQLTYEMWRAVRLVVDTGMHSKQWTREQAIEFF
                     RENTAKTELDIVNEVDRYISWPGQALAYKIGELKIKELRDRAKERLGDKFDIREFHDA
                     ILRQGAVPLDVLEQIVEEWLEKSGA"
     gene            774..1583
                     /locus_tag="VFV87_07155"
     CDS             774..1583
                     /locus_tag="VFV87_07155"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103569.1"
                     /translation="MTLLRLAMWSGPRNISTAMMRSWGNRSDTVVCDEPLYAYYLNAT
                     RKPHPGLEEVIAAGETDWRKVVEWLTDRRPLAPRDAMEPPFLRDGAPANSPNRISRSE
                     VPTIFYQKHMTHHLLPEVSREWLGRVTNCFLIRHPREMLASYVKIVEVPELEDTGFPQ
                     QTEIFEWVRQHTGRMPPILDSADVLQDPRRMLGLLCDALGTSFQEAMLAWPPGPRATD
                     GVWAKYWYGEVLKSTGFRPYQPKHIELPDWLQPVYRNCLNHYERLYELRIR"
     gene            complement(1610..1873)
                     /locus_tag="VFV87_07160"
     CDS             complement(1610..1873)
                     /locus_tag="VFV87_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5103570.1"
                     /translation="MPTIFVPPQLRELTCGQAEVQAQGATVREIVAALEKRFPGLEAR
                     LCRAGELSPALQVSIDGVLHRSGLQARVQPSSEVHFLPVFGGG"
     gene            complement(1894..2763)
                     /locus_tag="VFV87_07165"
     CDS             complement(1894..2763)
                     /locus_tag="VFV87_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX5103571.1"
                     /translation="MSLDSSTQRLSLGQCATLACLLEATAPKAGNVHRGADFDDLTFH
                     DFAASAVAIAPVIDAAAQRGIGRTILDAISATRALVATNTNLGIVLLLAPLAAIPRDE
                     PLTAESARGVLSRLTADDCRYVYEAIRLARPGGLGKAPEMDVASEPPADLLAAMRAAA
                     DRDLVARQYADDFRLVLDEALKLLIAGRQQGWSLTDAIIHTHLQLLARYPDSLIVRKC
                     GRETAAQASNMAGSVLEAGNPGDEPYFEALADFDFWLRADGHRRNPGTTADLLAAALF
                     AGLRDHRLRPPYR"
     gene            complement(2805..>3371)
                     /locus_tag="VFV87_07170"
     CDS             complement(2805..>3371)
                     /locus_tag="VFV87_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5103572.1"
                     /translation="LGTVQRLPQRFARIVLFNTAAFPPPFVPWRIAACKLPLVGTFAV
                     RGLNAFARGALSMAVEKPERMTADVRAGLLAPYDNWANRVAVDRFVRDIPFSRRHPTW
                     KTLEHIEAGLPSLADRPIQLLWGLRDWCFRPECLQRFWQHWPDAEVHELADCGHYVVE
                     DAHERIVPLVREFLVAHPVGKEVKAAAD"
BASE COUNT          632 a         1037 c         1120 g          582 t
ORIGIN      
        1 aaatgcggcc aaaatacgaa atggaggctc tctcgctcca cgaagcggta ccgggccacc
       61 atctgcagct tgccctgcag gccgagctgg gcgagctgcc gccgtttcgc cgctacggcg
      121 ggtttacggc ctatatcgaa gggtggggcc tgtacgcgga aagcctcggc gatcagctcg
      181 gctgctacca ggacccgtac tccaagtttg gccagctcac gtatgaaatg tggcgggcgg
      241 tgcggctggt cgtcgatacg ggaatgcaca gcaagcagtg gacccgcgag caggccatcg
      301 agttcttccg cgaaaacacc gccaagaccg agctggatat cgtcaacgaa gtcgatcgct
      361 atatctcctg gcccggacag gcgttggcct acaagattgg cgagctgaag atcaaagagc
      421 tgcgcgaccg tgctaaagaa agactcggcg acaaattcga catccgcgag ttccacgacg
      481 ccattctccg ccaaggcgcc gtgccgctgg atgtcctgga gcagatcgtc gaggagtggc
      541 tggaaaagtc gggtgcctga cgctgcggcc gcgacgcacc aggcctgata ctgtgatcgc
      601 tcgttcccac gctccgcgtg ggaacgcccg cccggacact ctgcgcccgg gattcgacgc
      661 agagcgtcag aatgtgggtt cccacgcaga gcgtcggaac caggagcgca aaccaggagc
      721 gcgacgtcga gaatggtgca ttgcgcttcg cttatgcacc ctacaatgaa tgaatgactc
      781 tgctccgtct cgccatgtgg tccggaccgc gcaacatctc cacggcgatg atgcgctcgt
      841 ggggcaatcg cagcgacaca gtcgtctgcg acgagccgct ttatgcgtac tacttgaatg
      901 cgacccgcaa gcctcatccg gggttggaag aagttatcgc cgcgggcgag accgattggc
      961 ggaaggtggt cgagtggctc accgaccgta ggcctctcgc tccgcgagac gcaatggagc
     1021 ctccattctt gcgcgacggc gcgccagcaa acagccccaa tcgcatctcg cggagcgagg
     1081 tgcctacgat tttctaccag aagcacatga cccatcacct gctgccggag gtgagccgcg
     1141 agtggctggg ccgcgtgacg aactgcttcc tgatccgcca tccgcgcgag atgctggcat
     1201 cgtacgtgaa gatcgttgaa gtgccggagc ttgaagacac cggctttccg cagcagaccg
     1261 agatatttga gtgggtccgc cagcacacgg gtcgcatgcc gcccatcctc gactcggccg
     1321 acgtgctgca ggacccgcga cggatgctgg gcctcttgtg cgatgcgctg ggcaccagct
     1381 tccaggaagc gatgctcgcc tggccgcccg gaccgcgggc caccgacggc gtctgggcca
     1441 aatactggta tggcgaggtc ttgaaatcga cgggctttcg gccgtatcag cccaagcaca
     1501 tcgagctgcc cgactggctc cagccggtgt atcggaattg cctgaaccac tacgagcgtc
     1561 tctacgagct gcggattcgt tagggagctc ggagggcgga tcggcggaat cagccgcccc
     1621 cgaatacggg caggaaatgc acctcgctcg acggctggac gcgagcctga agtccgctgc
     1681 gatggaggac tccgtcgatc gacacctgca gtgccggcga tagttcgccc gctctgcaaa
     1741 gtcgcgcctc caagccggga aatcgcttct ccagcgctgc gacgatctcg cgcaccgttg
     1801 cgccttgcgc ctgcacttcg gcctggccgc aggttaattc acgaagttga gggggaacga
     1861 agattgtggg cataggtatt ggattgtgcg ggcttaccga tagggcggac gcaaacgatg
     1921 atcgcgcaga ccggcaaaca gggccgcggc gagaaggtcg gcggtggtgc cgggattgcg
     1981 gcggtggccg tcggcacgca gccagaagtc gaagtcggcc agggcctcga aatagggctc
     2041 gtcgccggga ttgcccgctt ccaggacgct gccagccatg ttggaggctt gggcggccgt
     2101 ttcgcggccg cacttgcgga caatcaggct gtcgggatag cgggcgagca gttgcaaatg
     2161 ggtgtgaatg atcgcgtcgg tcagcgacca gccttgctgg cggccagcga ttagcagctt
     2221 cagtgcctca tccaatacga ggcgaaagtc gtcggcatac tggcgggcga caagatcgcg
     2281 gtcggcagca gcacgcatgg cggcgagcag atcggcgggc ggctcgctgg cgacatccat
     2341 ctccggcgct ttgcccagcc cgcccggccg cgcgaggcgg atcgcctcat agacgtagcg
     2401 gcaatcgtcg gccgtaagcc gtgacagcac gccgcgagcg ctctcggccg ttaacggctc
     2461 gtcgcgcggg atagctgcca gcggcgcgag cagcaggacg atgcccaggt tcgtgttggt
     2521 tgccacaagc gcccgcgttg ccgagatcgc atcgagaatg gtgcgaccga tgccgcgctg
     2581 ggcggcggca tcgatcaccg gcgcgattgc tacggcgctg gctgcgaagt cgtgaaacgt
     2641 caagtcgtcg aaatccgctc cgcgatgcac gttgccggcc tttggcgcgg tcgcttccag
     2701 caggcaagcg agcgtggcgc actggccaag cgagagtctc tgtgtagatg aatcaagtga
     2761 cattcactac tgttgtcttc tttgcgttct tggcgtcctt ctgttcaatc tgccgcggcc
     2821 ttgacttcct ttccgacagg atgcgctaca aggaattcgc gcaccagggg gacgatccgc
     2881 tcgtgggcgt cttcgacgac gtagtggccg cagtccgcca gctcgtggac ttccgcgtct
     2941 ggccagtgct gccagaatcg ctggaggcac tcgggccgga agcaccagtc gcgcaggccc
     3001 caaaggagct gtatcggccg gtcggccagc gacggcaggc cggcttcgat atgttcgaga
     3061 gttttccaag tcgggtgccg ccgcgagaac ggaatgtcgc gcacaaagcg atcgacggcc
     3121 acgcggttgg cccagttgtc gtagggggcc agcagacccg cccgcacgtc ggctgtcatc
     3181 cgctcgggct tttcgaccgc catcgacagc gcgccgcggg caaacgcatt caggccgcgg
     3241 acggcgaatg tgcccacgag cggcagcttg caggcagcga ttcgccaggg gacaaacggc
     3301 ggcgggaagg cggccgtgtt gaagaggacg atccgcgcga aacgctgcgg cagtcgctgg
     3361 actgttccca g
//
LOCUS       DASUGB010000372         5989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1986923, whole genome shotgun sequence.
ACCESSION   DASUGB010000372 DASUGB010000000
VERSION     DASUGB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986923"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1630
                     /locus_tag="VFV87_07175"
     CDS             320..1630
                     /locus_tag="VFV87_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5103573.1"
                     /translation="MNDAASQPMPGDATLAPPSRGSLLVIFLTVFIDLLGFGMVLPLL
                     PLYARRFGVDEHGWELGLLMASFSAMTFLFAPLWGRLSDRIGRRPVIVVGLAASAVFY
                     GVFGLAATVGGQAGLAWLFFARIGAGIAAGTIPTAQAYIADVTPVGKRTKGMALIGIA
                     FGLGFTFGPLIGYAALVFSKSESEIATSPWPGYAAAMLSGLACLLALALLPESLRPGK
                     RTEHHSFFDWRGWSDALHTPSIPAILITAFVSVVSFGAYETTLALLLNSSRFGFRFEQ
                     ILLYFAFIGLTLSVTQGALVRPLASRVSEVMMATAGVFVTILGFLLLSLATQQSRLPL
                     LIIASAVEVTGFSLITPSVQSLVSRRSDPAKQGGILGVSQGTSALARVVGPLIAVPLF
                     FRSPEWPYYAAIAMMAVALAVFLLFARHGKDYGTTDAASELPSL"
     gene            complement(1723..3264)
                     /locus_tag="VFV87_07180"
     CDS             complement(1723..3264)
                     /locus_tag="VFV87_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEX5103574.1"
                     /translation="MTTIDLQPRDEYNARLLANAHPADWQNPQPRALYDLVVIGGGTA
                     GLVSAAGTAGLGGQVALIERHLLGGDCLNYGCVPSKALIRAGRIAHEVRGAAGFGVRG
                     SGEGAGATIDFPTVMSRMRRLRAEISPHDSAQRFAGLGVDVFLGDARFTDPQSVEVAG
                     SALRFKRCIIATGGRAAVPGISGLAECGYLTNETVFSLTELPPRLIVLGGGPIGCELA
                     QTFRRFGSQVTLVQRASRLLPRDEPEAAEILDRQFRREGITLLYNARATACLPQASRA
                     AKRLVIQAGGQEHVLEADAILIAAGRTPNLEGLNLAAAGANFNKRGVEVNDFLQTANP
                     RIYAAGDIAGSYQFTHAADAMARICIQNAFFSIGPFGKKRLSKLVVPWTTYTDPEVAH
                     VGLTPPAAEEQGIAIDSYREEMSRVDRAILDGQTDGYAVIHCKRGTGNVVGCTIVAAH
                     AGDLIAEVALLMTAGLPLGTLARTIHCYPTQAEVLKCLGDQYNKTRLTPRAAALLKTI
                     IGWRL"
     gene            complement(3396..4535)
                     /locus_tag="VFV87_07185"
     CDS             complement(3396..4535)
                     /locus_tag="VFV87_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103575.1"
                     /translation="MNSGCLHVAALAGLAALAGCSGPQVRAHIDSVNAEYRQLEDYVY
                     VLEDENARLQRRIDHVQDNYERALRGEAPRRPGPFERRTDGSGDADSLSPPTIEGGTP
                     MEPPTIEIPGRSTTQRPELDSKSAPSSGRETLDLSPPAAGVPDASDGHSPETGDLPTP
                     AVPRQLNAPAPEVLPKLPADPQVTHLFLNPLMTGGADFDRQPGDEGLSIVLEPRNAAD
                     EYVPQAGAVSVVVLDPARQGEAARIARWDFTLSATQQKLQDASAAPGICLEMPWPASP
                     PTNNRLKLYVRYETADGRQLQTDREIFITPPGQLAQRWTPRPPDRLQAPAAAPQTSAP
                     ATSPPASVISQLPAAESAPKLLQPPAALSSEAPQKLAAPQWSPYR"
     gene            4769..>5989
                     /locus_tag="VFV87_07190"
     CDS             4769..>5989
                     /locus_tag="VFV87_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX5103576.1"
                     /translation="MASDNHDDDPSDQESKKSPTRAAGPGLLIPLVLIAILAGIFLLR
                     NMGPERTEIAYSFFIEQVRAKNIVEVELGDPLTFGRFKKPPVLPEEPPPADQTPADTT
                     ETKPAKAAAKTGVDAKKKEAPKRAKEHFYVKLTEGVKDSEWFQQLLVDNVPVVRNQIK
                     YDATPWILGIGMAVVIGMSLFFFLWVRRQQNQMMGGGFLSGFGKSPAKRYEASRQAVT
                     FKDVAGIESVKADLMEIVEFLKNPKKFQKLGGRVPKGCLLNGPPGTGKTLLARAVAGE
                     AGVPFFAVSGSEFIQMFVGVGASRVRDLFKNAKENAPAIIFIDEIDAVGRQRGAGLGG
                     GHDEREQTLNQILSEMDGFTQTDFVIVLAATNRPDILDPALLRPGRFDRHISVGRPTQ
                     KGRIEIFKVHTRDVP"
BASE COUNT         1008 a         1891 c         2021 g         1069 t
ORIGIN      
        1 gccgcagctc ctcgcgcggg agctgctcca ttcggcggcg gtcggcggcg gtgaggcgca
       61 gcataaccgc atgataggag gggacagggg acgggggcag gggacgggag ccaggagtca
      121 ggagtcaggg gacaggagac aggagacagt ggggagttcc gcatgccaag caagtgagta
      181 ctgagtacca agtactgagt actcaacttg ccaagtcaat ttgcgatccg aaatctacaa
      241 ccctccctct atcacctcat gttccgttcg ccggtccgcc ccctgactcc cgtcgcgtgc
      301 ccctgctatc ttcgtgcgga tgaacgacgc cgcctcccag ccgatgcccg gcgacgcgac
      361 cctcgcgccc cccagccgcg ggtcgctgct ggtgattttc ctgacggtgt tcatcgacct
      421 cttgggcttc ggcatggtgc tgccgctgtt gccgctctac gccaggagat ttggcgtcga
      481 tgagcacggc tgggaactgg gactcttgat ggccagcttt tcggcgatga cgtttctgtt
      541 cgcaccgctc tggggtcggc tatccgaccg cattggtcgg cggccggtaa ttgtcgtcgg
      601 gctggcggct tcggctgtgt tttacggggt gttcggcctg gcggcgaccg tcggcgggca
      661 agccgggttg gcatggctgt ttttcgcccg cattggcgcg ggaatcgccg cggggacgat
      721 cccgacggcg caggcctata tcgccgatgt cacgccagtg ggaaagcgaa ctaagggaat
      781 ggctctgatt ggcatcgcgt tcggtttagg attcacgttt ggcccactca tcggctatgc
      841 ggccttggtg ttttcaaaaa gtgagtcaga aatcgccacc agcccatggc cggggtatgc
      901 cgccgcgatg ctctcgggcc tcgcctgcct gctcgcgctt gcgctgctgc cggagtcgct
      961 gcggccggga aagagaacgg agcatcactc gtttttcgac tggcggggct ggtcagacgc
     1021 cctccacacg ccgtcgattc ccgccattct catcactgca tttgtaagcg tggtgtcgtt
     1081 tggagcgtat gaaacaacat tggcactcct gctgaatagc agccgctttg gattcagatt
     1141 cgagcagatc ttgctctatt ttgcgttcat tggcttgacg ctcagcgtga ctcaaggggc
     1201 gctggtgcgc ccgctcgcca gccgggtgag cgaggtgatg atggcaaccg ccggagtctt
     1261 tgtgacgatc ctgggcttcc tgttgctcag cctggcgacg caacagagcc ggttaccgct
     1321 cctaatcatt gcgtcggccg tcgaggtgac gggcttttcg ctgatcacgc cctcggtgca
     1381 gtcgctggtc agccggcgga gcgaccccgc caagcaaggg ggcattctcg gcgtgagcca
     1441 aggcacgagc gccctggccc gcgtcgtcgg ccccttgatc gcggtgccgc tctttttccg
     1501 ctcgcccgag tggccgtact acgccgcgat cgcgatgatg gcggtggcgc tcgcggtatt
     1561 cctgctcttc gcacggcatg ggaaggatta cgggacaact gacgcggcaa gcgaacttcc
     1621 atctctttag ccgggagcaa acttgctccc ggagcggcgc gagttcgcat ggatcgtcga
     1681 acggcagttc tccgggacca ggtttggtcc cggctaggaa catcacaagc gccagccgat
     1741 gatcgttttc aagagagcgg cggcgcgggg cgtgaggcgg gtcttgttgt attggtcgcc
     1801 gaggcatttg aggacttcgg cttgcgtggg atagcagtgg atcgtgcggg cgagcgtgcc
     1861 aagtggcagg ccggcggtca tcaggagggc gacttcggcg atcaggtcgc ccgcgtgggc
     1921 ggcgacgatc gtgcagccga cgacgttgcc cgtgccgcgc ttgcagtgaa tcaccgcgta
     1981 gccatcggtc tggccgtcaa ggatcgcccg atcgacgcgg ctcatttctt cgcgataact
     2041 gtcgatggcg atgccttgct cttcggcggc tggcggcgtg aggccgacgt gcgcgacttc
     2101 cgggtcggtg taggtggtcc acggcacgac gagcttgctg agccgtttct tgccgaaggg
     2161 gccaatggag aagaacgcgt tctggatgca gatgcgagcc attgcgtcgg cggcgtgcgt
     2221 gaactggtag ctgccggcga tatcacccgc ggcgtagatc cgcgggttgg cggtctgcag
     2281 gaagtcgttg acttcgacgc cacgcttgtt gaaattcgct ccggctgcgg cgaggttcag
     2341 gccctcaaga ttcggcgtgc ggccggcggc gatgaggatg gcgtcggcct caaggacgtg
     2401 ctcttgcccg ccggcctgga taaccagtcg ttttgcggca cggctggcct gcggcaggca
     2461 agccgtggca cgggcgttgt agagcagtgt gatgccctcg cggcggaatt gccggtcgag
     2521 aatctccgcc gcttccggct cgtctcttgg caagagccgc gaggcgcgct gaacgagcgt
     2581 gacctggctg ccgaaacggc ggaaggtctg agccagttcg cagccgatgg gcccgccgcc
     2641 gaggacgatc agccgcggcg gcagttcggt gagggaaaag acggtttcgt tcgtgaggta
     2701 accacattca gctagaccgg atatgcccgg caccgccgcc cgcccgccag tcgcgatgat
     2761 gcagcgcttg aagcggagcg cgctgccagc gacttccacc gattgcggat ctgtaaagcg
     2821 cgcgtcgccc aagaacacat cgacacccag cccagcgaac cgctgggccg agtcgtgcgg
     2881 gctgatctca gcccgcaagc ggcgcatgcg ggacatcacc gtgggaaaat caatcgtcgc
     2941 gcctgcgccc tctcccgaac cccgaacccc gaatcccgcc gccccgcgca cctcgtgtgc
     3001 tatccgcccc gcgcgaatca gtgccttgct cggcacgcag ccgtagttga ggcagtcgcc
     3061 gcctaaaaga tgccgctcga tcagcgcaac ctggccgccg aggcccgccg tgcccgcagc
     3121 ggaaacgagg cccgcggtcc cgccgccgat cacgacgaga tcgtaaagcg cccgcggctg
     3181 cgggttctgc caatcggcag ggtgtgcatt ggccaggagt cgggcgttgt attcgtcgcg
     3241 cggctgaagg tcgatcgtgg tcatggccga gactcaccgc tgatagccgc acttgccaga
     3301 gtgcggggct gaaggggtcg ggagtcaatt gcggctaagt cggtccattt ggatggatct
     3361 cgaccgcaat tgactcccga ccccttaacg ccagcctagc gatacggcga ccactgggga
     3421 gcggccagct tttggggtgc ttcgctgctg agcgcggctg gcggctggag gagcttcggg
     3481 gcggattcgg cggcagggag ctgggaaatg acgcttgccg gtggagatgt cgctggagca
     3541 gacgtttgcg gcgccgcagc gggcgcttgt aggcggtctg gcgggcgcgg cgtccagcgc
     3601 tgcgcgagct ggccgggagg cgtgatgaag atctcgcgat ccgtctgcaa ttggcggcca
     3661 tcggccgttt cgtagcggac atagagcttc aagcggttgt tggtcggtgg cgaggcgggc
     3721 cagggcattt ccaggcaaat cccgggggcc gcggaagcat cctgcagctt ctgctgcgtg
     3781 gccgaaagcg tgaaatccca ccgcgcgatc cgggctgcct cgccttgccg ggccggatcc
     3841 aggacgacga cagacaccgc gcccgcctgc ggcacgtact cgtccgccgc gttccgcggc
     3901 tccaggacga tgctcagccc ctcgtcgccc ggctggcggt cgaaatcggc cccgccggtc
     3961 atcagcggat tcagaaacaa gtgcgtcacc tgcgggtcag ccggcagctt tggcaagact
     4021 tccggagcag gtgcattcaa ctggcgcggc acggccggtg tgggcagatc gccagtctct
     4081 ggcgagtggc cgtctgaggc gtcaggaacg ccggccgcag gcgggctcag atcgagcgtt
     4141 tcgcggccgg acgacggagc actcttcgaa tccagctccg gccgctgcgt ggtcgagcgg
     4201 cccggaatct cgatcgtcgg cggctccatc ggcgtcccgc cttcgatcgt cggcgggctc
     4261 agcgaatcgg catcgcccga tccatccgtc cgccgctcga acggcccagg tcgccgcggc
     4321 gcctcgccgc gcagcgcccg ctcgtagttg tcctgcacgt ggtcgatccg ccgctgcagg
     4381 cgggcgttct cgtcctccag cacatagaca taatcctcga gctgccgata ctccgcgttg
     4441 accgaatcga tgtgggcgcg cacctgcggc ccggaacagc cggccaatgc agccagcccc
     4501 gccagcgcag caacgtgcag acagcccgag ttcatcgacg aatcccaatg cggcagccgg
     4561 ccccgattcg atcccgcgag aggccggcct cgcgcctgaa aagcggggcg gcttgtaccg
     4621 cgccggcgga attggcggaa gagcagccgc gctgccgagc tagcgtggca tgcagccagt
     4681 gctgcgaatc tggcgggggt acaccgcttg gccggcgtag ctagaatagg gtgacttcag
     4741 gagttgctgt ccgcgcgaag gaatctcgat ggcgtccgac aatcacgacg acgatccgtc
     4801 cgaccaggaa tcgaagaaaa gcccgacccg cgccgccggg ccggggctgc tgattccgct
     4861 ggtgctgatc gccatcctgg ccggcatctt cctcttgcgg aacatgggtc cggagcggac
     4921 ggagatcgcc tatagctttt tcatcgagca ggtccgggcg aagaacattg tcgaagtcga
     4981 gttgggcgac ccgctgacat ttggccggtt caaaaagccg ccggtccttc ccgaggagcc
     5041 gccgccggcc gaccaaacgc cagcagacac gaccgaaacc aaacccgcca aggccgccgc
     5101 caaaacgggc gttgatgcga agaagaagga agcccccaag cgggccaagg agcacttcta
     5161 cgtcaagctg accgaggggg tcaaagacag cgagtggttc cagcagttgc tggtcgacaa
     5221 cgtccctgtc gtccgcaacc agatcaaata cgacgccact ccctggatcc tcggcatcgg
     5281 catggccgtg gtcatcggca tgtcgctgtt cttcttcctc tgggtccgcc gccagcaaaa
     5341 ccagatgatg gggggcgggt tcctcagcgg cttcggcaag agccccgcca agcggtacga
     5401 agcttcgcgg caggcggtca cgttcaagga cgtggccggc atcgagagcg tcaaagccga
     5461 cctgatggag atcgtcgagt tcctgaagaa tccgaagaag ttccagaagc tgggcggccg
     5521 cgtgcccaag gggtgcctct tgaacggccc gccgggcact ggcaagactt tgctcgcccg
     5581 ggcggtcgcc ggcgaagcgg gcgtgccatt cttcgccgtc agcggctcgg aattcattca
     5641 aatgttcgtc ggcgtcggcg ccagccgcgt ccgcgatctg ttcaagaacg ccaaggagaa
     5701 cgccccggcg atcatcttca tcgacgagat cgatgccgtc ggtcgccagc gcggggccgg
     5761 gctcggcggc gggcacgacg aacgcgagca gacgctcaat cagatcctca gcgagatgga
     5821 cggcttcacg cagaccgact tcgtgatcgt gctggccgcg acgaatcgcc cggacattct
     5881 cgaccccgcc ctgctccgcc ccggccgctt cgaccggcac atcagcgtcg gccggccgac
     5941 gcagaagggc cgcatcgaaa ttttcaaggt ccacacccgc gacgtcccg
//
LOCUS       DASUGB010000373         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1709276, whole genome shotgun sequence.
ACCESSION   DASUGB010000373 DASUGB010000000
VERSION     DASUGB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709276"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFV87_07195"
     CDS             complement(<1..232)
                     /locus_tag="VFV87_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103577.1"
                     /translation="MSAADHQAAWRRLPKFANVRSLLEGSFRPASSHPSLILFTVNKA
                     ASSFVGNVLHELGLACSKAPVDLAGYGRELPPQ"
     gene            complement(445..1668)
                     /locus_tag="VFV87_07200"
     CDS             complement(445..1668)
                     /locus_tag="VFV87_07200"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5103578.1"
                     /translation="MDVEAIRRDFPILSTMLGDGCPLVYLDNAASTQRPTQVIEAMAD
                     AYEHYYSNVHRAGHRLAMESSAHYEAARESVRQLINARSPTEIIFTPGTTAAINLVAR
                     SWGDANLQPGDEILLTLMEHHSNIVPWQQLAARTGATIRWTPITDDYQLDLDAFERLL
                     SSRTKIVAVAAVSNVLGTINPIDPIIELAHAAGAVVLIDAAQAVPHEPVDVRARNVDF
                     LAFSGHKMLGPSGVGVLYGREDLLDRMQPFHGGGSMIKSVTTEGFTPADLPYRFEAGT
                     PVIVPAIGLGRAIQYLQKLGMESIRAHEYALTKQAHALLADIPGLRLLGPAPEKKGGI
                     VTFVLEGIHPDDVSKALDIQGIAVRSGHHCAMPLHTRLGLTASTRASFYLYNTPQEVA
                     RLAEALRGAQRLFQR"
     gene            complement(1870..2256)
                     /locus_tag="VFV87_07205"
                     /pseudo
     CDS             complement(1870..2256)
                     /locus_tag="VFV87_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
     gene            2399..>2670
                     /locus_tag="VFV87_07210"
     CDS             2399..>2670
                     /locus_tag="VFV87_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103579.1"
                     /translation="MSRIGALALLLASWCGAVSVAAADPLPEKIVVATWNVEWLFDNY
                     TGDNPNELARSQSAPSREAWDWKLAGVAKVIAEIKPTILALQEVEN"
BASE COUNT          459 a          793 c          938 g          480 t
ORIGIN      
        1 cctgtggagg aagttcacgc ccataacctg ccagatcgac tggtgctttg gagcaggcaa
       61 ggcccagctc gtgcaacacg ttcccaacaa acgaagacgc ggccttgttg accgtgaaca
      121 ggatcaggct ggggtgtgag ctagcgggcc tgaacgaccc ttccagcagc gaccggacat
      181 tggcaaactt cgggagccga cgccaggcgg cttggtgatc tgcggcgctc atggagaaag
      241 aatgctagct attgggcgta agcgaatcga atgccagact cgtgccggta acatcggcgg
      301 cagcggttgt agcaattcaa gcagactggc aaaatcggaa aagtttttaa ggtggtgcac
      361 gtggaatccg agaagaatcc gccgccgccg ctttggtcat tacgctgggg aaccggaggg
      421 cgtggtcgat cggcgccgga tcggctatcg ctgaaacagg cgttgggcgc cgcggagggc
      481 ttcggccagg cgggcaacct cttgcggggt gttgtagagg tagaagctgg cccgcgtgct
      541 agcggtcagc cccaggcgcg tgtgcagcgg catggcgcag tgatgcccgg agcggaccgc
      601 aatgccctgg atatcgaggg ctttggaaac gtcgtcgggg tggatgccct ccaggacgaa
      661 cgtgacgatg cctcctttct tctccggcgc gggacccagg aggcggagcc ctggaatgtc
      721 ggctaaaagc gcatgggcct gtttggtgag cgcgtattca tgggcccgga tgctctccat
      781 ccccagcttt tgcaggtact gaatcgcgcg gcctagtcca atggccggga cgatgaccgg
      841 cgtgccggcc tcaaagcggt aaggcaggtc ggcaggcgtg aagccctcgg tggtgacgct
      901 cttgatcatg ctgccgccgc cgtggaacgg ctgcatgcgg tcgagcaggt cctcgcggcc
      961 gtagagcacg cccacgcccg acggcccgag catcttgtgc ccgctgaagg ccaggaaatc
     1021 gacgttccgc gcccggacat cgaccggctc gtgcggcacg gcctgggcgg cgtcgatcag
     1081 gacgacggcg ccggctgcgt gggccagctc gatgatcgga tcgatcggat tgatcgttcc
     1141 cagaacgttg gaaaccgcgg ccacggcgac gatcttcgtc cgcgacgaga gcagacgctc
     1201 gaaggcgtcc aggtcgagct gataatcgtc ggtgatcggc gtccagcgga tcgttgcgcc
     1261 ggtgcgggcc gcgagctgct gccaggggac gatgttgctg tggtgctcca tcagcgtgag
     1321 caggatttca tcgccgggct gcaggttcgc atcgccccag ctgcgggcga cgaggttgat
     1381 cgcggcggtc gtgccgggcg tgaagatgat ttcggtcggg ctgcgggcgt tgatgagctg
     1441 ccgcaccgat tcgcgggccg cttcgtagtg ggcgctcgac tccatcgcca ggcggtggcc
     1501 ggcgcggtgg acgttgctgt agtagtgctc gtaggcgtca gccatcgcct cgatcacctg
     1561 cgtcggccgc tgcgtcgagg cggcgttatc cagatagacc agcgggcagc catcgccgag
     1621 catcgtcgaa aggatcggaa agtcgcggcg gatcgcctcc acatccagcg gcgaagccgc
     1681 ggccggctgg ccggtgacga gcgtcgccgg caagagcggc tgcggtttcg gccggccgtt
     1741 gccgccggtc ttattgccgc cggtcttgtc gtcggacttc ggcttgagcg gcacgatcgg
     1801 cagcgcgggc gacgtggatt gcggcgtacc ttccctgtcg gcggccagtt tggcaatggc
     1861 ctgaatccgc cggaccatcg ccgtcaggcc gttgctcctc atgggcacga gggccggcag
     1921 cttcagctcg gcgaacagct tatcaatcgg aaacgcgacg atctccgccg gcgtgtgccc
     1981 cgaaaaagcg gccagcagca cggcaatcag cccgcggacg atctgggcgt cgctatcggc
     2041 ggcgaactcc agcgtcggcg gtgtagttgg ccgcgcctcc ggcaccagcc atacccgcgc
     2101 ctggcagccg agaacgcggt tctcctcggt cttcaggctc tcgtccatcg gcgggagggt
     2161 gcggccgatc tcgatcaggt acgtggtgcg atcgaccgga tcgtcgaggt cggcgaactc
     2221 cgcgcgaatt tcgtgcagtg ggacggcaac agtcatcgaa cagggttttt ctggcggtgc
     2281 ttttgcactc gccgcactat ccagccgctt gcgggccaag taccgtaagg tggcgttgat
     2341 ctgtctcaat tataaacggc cgggcgggga ccgtctgggg gccgcggaga gctgctttat
     2401 gagtcgaatc ggggcgcttg ccttgctgct ggccagttgg tgcggcgccg tttctgtggc
     2461 tgccgctgat cccttgccgg aaaaaatcgt cgtggcgacg tggaacgtcg aatggctgtt
     2521 cgacaattac acgggcgaca atccgaacga attggcccgc agccaatcgg cccccagccg
     2581 cgaggcgtgg gactggaagc tggctggcgt cgccaaagtg atcgccgaaa tcaagccgac
     2641 gatcctggcc ctgcaggagg tcgagaacaa
//
LOCUS       DASUGB010000374         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_876497, whole genome shotgun sequence.
ACCESSION   DASUGB010000374 DASUGB010000000
VERSION     DASUGB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876497"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFV87_07215"
     CDS             complement(<1..1036)
                     /locus_tag="VFV87_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103580.1"
                     /translation="MTRFLGWLLGIENATQIDGIDVSLAAPWAQDGLFWVVLAAGGLI
                     VLALFFYLRLQAKGPAGPRLALGIFRGVLLALLLLMLADPVLQLTVTSRQQPFLYVIF
                     DGTDSMAIEDELPDAQRTAVEKAVGWTGGGAAASKPGTTSTSSGNPATGGGGGADREE
                     AEGAAPSRMDYVQSLLRKEDENLLRRLAEEKQVQLEAFLFDGESTSQLRKLNLNASGD
                     RQIDPKYLADQLSTKGKVTALGSVVHEIGQQFGAGRLAGVVVVSDFAHNSGASPLGTG
                     GQSPVSRLGAPIYAVGVGATEAVDLAVDVQTDPKMKKAERSSVLVKLRQSGLTGQTVT
                     VRVSAKKLGGD"
     gene            complement(1033..2199)
                     /locus_tag="VFV87_07220"
     CDS             complement(1033..2199)
                     /locus_tag="VFV87_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103581.1"
                     /translation="MTARQTLNLLGFVLLLAAAGCWGSATAPKSLPPPRAANAGAQQK
                     ATPAADATSERPGRASKVTDVATQSAESPSQQPSADAETAESPENPPQAAAAVSKERI
                     VLLAPLNPFLIEFHFTIDGQPHEQALERLVDAVIKLADTDGDGRPMWAEVTSSKRFKY
                     GQFGNLAINNDNDHKQIVERYDIDRDKVVDRDELPRFLTRNAGGSRSFSIRGTADYRG
                     MNRRGAPTWQVIDTDGDGRISPEERQSAAGELRIRDSDDDEILVATDLNPRAALGDPG
                     MMSMRRRAGPQAARLLGPHADWDNVRLALEHDYGGSRYLETDSFPLTPQLFTELDTNK
                     DGRIDRTEIRGLDSVPPHMVIAVDFGKEPPKADASSPLSPGGRGVGGEGEVAVA"
     gene            2387..>2707
                     /locus_tag="VFV87_07225"
     CDS             2387..>2707
                     /locus_tag="VFV87_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX5103582.1"
                     /translation="MGLPARRIHQQFSLVSSLYRFMDAASVAGGLALAAWTARVPIGD
                     YLLPGAIAIIVHYLFAELAGMYRSWQGVSGNREAYLRLSTWALAFLLILGIGLATERL
                     NDYPR"
BASE COUNT          440 a          906 c          849 g          512 t
ORIGIN      
        1 aatcgccgcc gagtttcttg gccgataccc gcaccgtcac ggtctgcccg gtcagcccgc
       61 tctgccgcag cttgaccagt acgctgctcc gctcggcttt cttcatcttg ggatcggtct
      121 gcacatcgac ggccagatca accgcttcgg tagcgccgac gccgaccgca tagatcggcg
      181 cgccgagacg gctgacgggc gactggccgc ccgtgccgag cgggctggcg ccggagttgt
      241 gggcgaaatc gctgaccacc acgacacccg ccaggcggcc ggcgccgaac tgttggccga
      301 tctcatggac gacggagccc agtgcggtga ccttgccctt cgtcgaaagc tgatcggcca
      361 ggtatttagg atcaatctgc cggtcgccgg acgcattgag attgagcttg cgaagctggc
      421 tggtcgattc gccgtcgaag aggaaggctt cgagctgcac ctgcttctcc tcggccagac
      481 ggcggagcag attctcatcc tccttgcgca ggagcgactg gacatagtcc atgcgggagg
      541 gcgctgcgcc ctcggcttcc tcgcggtcgg cgccgcctcc gccgcccgtt gcgggattgc
      601 ccgaggaagt cgatgtggtt cccggtttcg aggcggccgc accaccgccc gtccagccga
      661 ccgctttctc caccgccgtt cgctgagcgt cgggcagctc gtcctcgatc gccatgctgt
      721 cggtgccgtc gaagatgacg tacaggaacg gctgctggcg gctggtgacg gtgagctgca
      781 gcacgggatc ggcgagcatc agcagcagca gcgcgagcag cacaccgcga aaaatcccca
      841 gcgccaagcg cggtccagcc ggccccttgg cctgcagccg caggtagaag aacagtgcca
      901 gcacgatcag cccgcccgcc gccaagacca cccaaaagag cccgtcttgc gcccacggcg
      961 cggcgagcga gacgtcgatc ccgtcgatct gagtcgcatt ctcaattccc aacagccagc
     1021 cgaggaagcg ggtcatgcga ctgccacctc cccctcaccc ccgacccctc tcccaccagg
     1081 ggagagggga gaggaagcat ccgccttcgg cggctccttc ccaaaatcca cggcaatcac
     1141 catgtgcggc ggcacactgt ccagaccgcg gatttcggtc cggtcgatgc ggccgtcttt
     1201 gttcgtgtcg agctccgtga agagctgcgg agtcagcggg aagctgtctg tctccaggta
     1261 tcggctgccg ccataatcgt gttcgagagc cagccgcacg ttatcccaat cggcgtgcgg
     1321 gccgagcagg cgggcggctt gcggaccggc gcggcggcgc atgctcatca tccctggatc
     1381 gccgagggca gcccgcgggt tcaggtccgt ggcgacgaga atctcgtcgt catcgctgtc
     1441 gcggattctc aattcgccgg cggcggactg ccgctcctcg ggcgagatcc ggccgtcgcc
     1501 gtcggtgtcg atcacctgcc acgtcggggc cccgcggcgg ttcatccccc gataatcggc
     1561 ggtgccgcgg atcgaaaacg accgcgagcc gccggcattg cgagtcaaaa atctcggcag
     1621 ctcgtcgcga tcgacgacct tatcgcggtc gatatcgtac cgctcgacga tctgcttgtg
     1681 gtcattgtcg ttgttgatcg ccaaattgcc gaattgacca tacttgaacc gcttgctgct
     1741 ggtgacctcc gcccacatcg gccggccgtc gccgtcggta tctgccagct tgatcactgc
     1801 atcgaccagc cgttccagtg cctgctcgtg cggctggccg tcgattgtga agtggaactc
     1861 gatgaggaac ggattgaggg gggccagcag gacaatccgc tccttcgaga ccgcggcagc
     1921 ggcttgtggc ggattttccg gcgattccgc cgtctccgca tcggcactcg gctgctggga
     1981 agggctttca gcagattggg tggcaacgtc cgtgactttg ctagctctgc ctggccgttc
     2041 cgacgtagcg tctgccgccg gggtcgcctt ctgctgtgcc ccagcgttgg ctgctcttgg
     2101 cggcggtagc gacttgggtg ctgtcgccga tccccagcag ccggcggctg ccagaagaag
     2161 cacaaatccg agcaaattca gcgtttgccg cgctgtcatc ctgagaactc cttgtgcgct
     2221 tgccgcattg taaccggtac tgacggcaga aagcgaacgt tcaatcgcgc caagccattc
     2281 acaagctaga ttcccctagc ggacgctcga tcccctctac ccgtaggacg gtttgccaag
     2341 ccgtctcagg cgggttgcaa acccgctcta cgatttggag catgccatgg gcctgcctgc
     2401 acgacgcatt caccagcagt tctccctggt cagctcactc tatcggttca tggacgccgc
     2461 cagcgtcgcg gggggcctgg cactggccgc ctggacggct cgcgtgccga tcggcgatta
     2521 cctgctgcct ggcgctatcg cgatcatcgt tcactattta ttcgccgaac ttgccggcat
     2581 gtaccggagc tggcaaggcg tcagcggcaa ccgcgaggct tatctgcggc tcagcacctg
     2641 ggccttggcg tttctgctca tcttggggat cgggctggcg acggagcggc tgaatgacta
     2701 tccgcgc
//
LOCUS       DASUGB010000375         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_459485, whole genome shotgun sequence.
ACCESSION   DASUGB010000375 DASUGB010000000
VERSION     DASUGB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459485"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..605)
                     /locus_tag="VFV87_07230"
     CDS             complement(42..605)
                     /locus_tag="VFV87_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5103583.1"
                     /translation="MGVTYFRRFRMEYDLSRPLFEEPRLPDGYSLVPWDDELLETHAE
                     VKFRCFRSELDANVFPSLGARDGCRRLMSEIAQRQGFVPEATWLLQSWPADDRKPELC
                     GTIQGVADDKLGAIQNIGITPAHRGKGLGSLLVWHSLAGFVRAGIPRVYLEVTAQNAG
                     ACRLYRRLGFQQTKVVYKASEVAYAEW"
     gene            827..1147
                     /locus_tag="VFV87_07235"
     CDS             827..1147
                     /locus_tag="VFV87_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103584.1"
                     /translation="MFRAQNCLRALLIVGGLALVSLPAAAQAGGGCVKFYGNGCHNFN
                     YNCQPTYCQPNYCQPSYCQPANCYPTTTCYPYTCQPIVVQPHCKPLCYPQFQSCQPII
                     IHKW"
     gene            complement(1272..1817)
                     /locus_tag="VFV87_07240"
     CDS             complement(1272..1817)
                     /locus_tag="VFV87_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103585.1"
                     /translation="MAIWFLRLSATLCFTTAAGALSLIGLDWYRHSADEAEYGVARQH
                     VSSLGGRIFKDDYGNYIIELQRSSVTDAELERLVLALQPLRSEHSSPDIERMFAFDLS
                     ATSVGDRGVQAIADLPLDWLNLNSTQITDGAFPHLYDQERLAVMTVSETHVTWPAIQE
                     MRAHSPHVWIPATLHGIGAQP"
     gene            complement(1933..>3417)
                     /locus_tag="VFV87_07245"
     CDS             complement(1933..>3417)
                     /locus_tag="VFV87_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX5103586.1"
                     /translation="EGLSYTEIWISEAQERMVLAVPEDKWPRLEALCQSENVEATVIG
                     RYVPTGKLELKYQGTSVGTLDMHFLHEGRPKVERQATYKPTAANPQSAIRNPKSSGVL
                     SLHADTTLLMLLATPNVASKEWVIRQYDHEVQGGSVIKPLVGVANDGPSDAAVLRPVL
                     SSRRGLAIACGLNPRYGDIDTYPMAACAIDEAVRNCVAVGADPARIAILDNFCWGYTD
                     RPETLGSLVRAALACYDVAVAYGTPFISGKDSLNNEFTYERRNATEGVPYSAGGQKQT
                     IQIPHTLLISALGQIEDVSRCVTMDLKSPGNLLYQVGRTKAELAGSHFELVTGASARN
                     LPGAGNVPQVDVETARRTFATLHKAIQAGHVRACHDLSEGGLSVAVAEMAFAGGCGAR
                     VRLAELPHDGSADSRSPEADAVLLFSESASRFLIEVPQAKRGPLEALFRQAQVPLAHI
                     GEVTDTSRLQIAASPHAGRELPPSWLIDLPIADLKEAWQRPLRW"
BASE COUNT          627 a         1060 c         1100 g          630 t
ORIGIN      
        1 ggcgggctgt ggcggcaatc tgaattggtg ctttcttacc cctaccattc cgcatacgca
       61 acctcagaag ccttatacac caccttggtc tgttgaaagc cgaggcggcg gtagaggcgg
      121 caggcgccgg cgttttgggc ggtgacttcc agatagaccc gcggaatgcc ggcccgtaca
      181 aagccggcca gcgagtgcca gacgagcagc gaccccagcc ccttgccgcg gtgggcgggg
      241 gtgatgccga tgttctggat cgcaccgagt ttgtcgtccg ccacgccctg gatggtgccg
      301 cacagttccg gcttgcggtc atcggccggc cacgattgca gcagccacgt cgcttccggc
      361 acgaagccct gacgttgggc aatctcgctc attagccgcc ggcagccgtc gcgcgccccg
      421 agcgatggga agacgttcgc gtccaattcg gagcgaaagc agcggaactt gacctcggcg
      481 tgcgtttcca gaagctcgtc gtcccagggg acgagcgaat agccatccgg caaccgcggc
      541 tcttcaaaga gcggccggct gaggtcgtat tccatgcgga aacgccggaa gtaggtgacg
      601 cccataaacc atccgcccag cagccgtggc gagaatcctc ctccgtactc tacgcggcgg
      661 tttgggggtg gtcaatttct tgccctggac tcgccctata ttccagctcg ccgcccaaaa
      721 cttgttgtaa caaccttcgc cgggatgcgc ctgaatgggt agcggcagcg gtcaaggtga
      781 ctgcagccct tcaacttgac ccatcacgct cgaaaggagt gttcccatgt ttcgcgctca
      841 aaactgcctg cgggcattgc tcatcgtcgg cggcctggcc ctggtttccc tgcccgcggc
      901 ggcgcaggcc ggcggcgggt gcgtgaagtt ctatggcaac ggctgccaca acttcaacta
      961 caactgccag ccgacgtatt gccaaccgaa ttactgccag ccgtcgtatt gccagcctgc
     1021 gaactgctat cccacgacga cttgctaccc gtacacctgc cagccgatcg tcgtgcagcc
     1081 acactgcaag ccgctctgct acccgcagtt ccaaagctgc cagccgatca tcatccacaa
     1141 gtggtaggcc ggcagcaggg cgactcgaca accagcaaca cgcggccccg agtgagcgat
     1201 cattcggggc tgctttgcgc gtgagacgaa cctcgcttca acaaagcaca cagttcaaca
     1261 aagcacacag ttcaaggctg cgcgccgatt ccatggagcg tagcgggaat ccaaacgtgc
     1321 ggcgaatggg cgcgcatctc ttgaatcgcg ggccaggtca cgtgcgtctc ggaaaccgtc
     1381 attacggcca gccgctcctg atcataaaga tggggaaatg ctccgtcagt gatctgcgtg
     1441 ctgttgaggt tgagccaatc cagcggcaaa tctgcgatcg cctgaacgcc ccggtctccg
     1501 acgctcgtgg ccgagaggtc aaaggcgaac atgcgctcga tatcgggcga cgagtgctcg
     1561 ctcctgagcg gctgaagcgc taaaacgagc cgctccagct cagcgtcggt gacggaactt
     1621 ctctgaagct cgatgatgta attcccgtaa tcgtctttga aaattctgcc gccaagcgat
     1681 gagacgtgct gccgggccac gccatactcg gcctcgtcag ccgagtgccg gtaccagtcc
     1741 aaaccaatca ggctcagcgc gcctgccgcc gtcgtgaaac aaagtgtcgc cgaaaggcga
     1801 agaaaccaga ttgccatggg acggtctccc gaagaggtgg ggaccagcgt ggatcaacat
     1861 agcatctact tccggcatcg caagcaagaa atcgacaatt ccagaggaat aacgattgac
     1921 ctgatagtcc ggctaccaac gcaggggcct ttgccaggct tctttgaggt cggcgatcgg
     1981 caggtcaatg agccagctcg gcggaagctc acggccggcg tggggcgaag cggcgatttg
     2041 caggcggctg gtgtcggtga cttcgccgat gtgggccagc ggaacctggg cttggcggaa
     2101 cagggcttcc agcgggccgc gcttggcttg cgggacttcg atgaggaagc gactggcgga
     2161 ttccgaaaag agcagcacgg cgtcggcttc ggggctgcgc gaatcggccg agccgtcgtg
     2221 cggcaattcc gccaggcgga ctcgtgcccc gcagccgccg gcgaaggcca tttcggccac
     2281 ggcgacggaa agtccccctt cgctgaggtc gtggcaggcg cggacatggc cggcttggat
     2341 cgctttgtgc agcgtagcga aagtgcgcct cgccgtttcg acatcgacct gcgggacgtt
     2401 gccggcgccg ggcagattgc gggcactggc gccggtcacc agctcgaagt gcgagccggc
     2461 gagctcggct ttcgtccggc cgacctgata gagcaggttg ccgggcgact tgaggtccat
     2521 cgtcacgcag cggctgacgt cttcgatctg gccgagagca ctgatgagca gcgtgtgggg
     2581 gatctggatg gtttgctttt ggccgcccgc gctgtaggga acgccctccg tggcgttccg
     2641 ccgctcgtac gtgaattcgt tattgaggct gtccttgccg ctgatgaagg gggtgccgta
     2701 ggcgacggcg acgtcgtagc aggcgagcgc cgcgcggacc agcgacccga gcgtttccgg
     2761 ccggtcggtg tatccccagc agaagttatc gaggatcgcg atccgcgctg gatcggcgcc
     2821 gacggcgacg cagttgcgga ccgcttcgtc gatcgcacaa gccgccatgg gataggtatc
     2881 gatatcgccg tatctcggat tgaggccgca ggcgatcgcc aggccgcgcc gcgaagagag
     2941 caccggccgc aggacagcgg cgtcgcttgg cccgtcgttg gcgacgccca ccagcggctt
     3001 gatcacgctg ccgccttgca cctcgtgatc gtactggcgg atgacccatt ctttactggc
     3061 gacgttgggc gtggccagca gcatcagaag cgtggtgtcg gcgtggagcg agagcacgcc
     3121 cgaggatttc ggattgcgga ttgcggattg cggattggcg gcagtcggct tgtacgtagc
     3181 ctggcgctcg accttcggcc ggccttcgtg caggaagtgc atgtcgagcg tgccgacgct
     3241 cgtcccttga tacttcagct ccagcttgcc ggtgggcaca tagcggccga tgacggtggc
     3301 ctcgacattc tcactttggc acagcgcctc cagccgcggc cacttgtcct cggggacggc
     3361 gaggaccatc cgctcctggg cttcgctgat ccagatttcg gtgtaggaga gcccttc
//
LOCUS       DASUGB010000376         6268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1293091, whole genome shotgun sequence.
ACCESSION   DASUGB010000376 DASUGB010000000
VERSION     DASUGB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293091"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFV87_07250"
     CDS             complement(<1..224)
                     /locus_tag="VFV87_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103587.1"
                     /translation="MRCWLVAGMTMACLGVAAGDAIAGNGQAKEKVHSWWYRSKVDWH
                     RNNAWPEPFASADRAAVREPFCLMIDNGWK"
     gene            complement(447..746)
                     /locus_tag="VFV87_07255"
     CDS             complement(447..746)
                     /locus_tag="VFV87_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103588.1"
                     /translation="MSSSDILLRRRAYRQASGENEQEPWRTRQSGWEEDPQQALFPRL
                     ADNGRAPLAALDLIVELRAALWAAASFELVYKLRPGARFHAGTFIFARQEGDLPS"
     gene            773..2098
                     /locus_tag="VFV87_07260"
     CDS             773..2098
                     /locus_tag="VFV87_07260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5103589.1"
                     /translation="MKIAMIGTGYVGLVTGTCLADSGNEVTCVDIDQAKIERLEHSEI
                     PIYEPGLSELVIRNAAAGRLKFTTKTADAVKGAKLIFLAVGTPTIGGPQGRGAADLKF
                     IWSAVEAAAPHLHSDAIVVIKSTVPVGTNAKIHSRLSGLTGRSCDVASNPEFLKEGAA
                     VNDFQYPDRVVVGVRTRAAEDALRELYSPFLRTDKPFLAMTPESAELTKYVANALLST
                     KISFINEMANLCERLGGDINDVRRGIGHDRRIGFAFLFPGVGYGGSCFPKDVRALAHM
                     AREAGIEPLLLDAVDEVNSRQKHMLILKLEQQFGGHLAGKHIALWGLAFKPKTDDIRE
                     APALVLIDHLLERGAKVAVHDPEAMDHVRQQYAERLNYSHSALDVLEGADALAINTEW
                     GEFRNPDFAEMRRRMKQPVIVDGRNLYDPDTMRASGFTYHSIGRVTVRP"
     gene            complement(2116..3171)
                     /locus_tag="VFV87_07265"
     CDS             complement(2116..3171)
                     /locus_tag="VFV87_07265"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5103590.1"
                     /translation="MLTVELPLGLAMGESAIQIPVENPLTRAKIELGRQLYFDSRLSA
                     DGTVSCASCHAPDAGYGKNTRFGVGIGGQEGGRNSPVSYNRILSSVQFWDGRAATLEE
                     QAKGPIVNPIEMGNTHEVCVATLKKIPGYLLQFDVIFPSKGISIDTVAKAIASFERAL
                     VTGPSSADYYEPLLAVGHAFRDDLEDLSAFQEDNPGAFKKYSALKAASEAHAISKSAR
                     RGRELFFGMKAKCTTCHVGANFTDEKYHNLGVGMDAAKPDLGRYEITKEEKDKGAFKT
                     PTIRNVVHSAPYMHDGNQKTLEEVVEWYDKGGHPNPQLSSKIKKLNFTPQEKQDLVAY
                     MKSLTGEFPRAETGHLP"
     gene            complement(3172..3384)
                     /locus_tag="VFV87_07270"
     CDS             complement(3172..3384)
                     /locus_tag="VFV87_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103591.1"
                     /translation="MPVFRALSASAWVLISVGLFASSVFAGDASAPAVLQTVLLGSSD
                     LTAGIPGKGPLTADQIKAWLANPKSH"
     gene            complement(3589..5511)
                     /locus_tag="VFV87_07275"
     CDS             complement(3589..5511)
                     /locus_tag="VFV87_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103592.1"
                     /translation="MKVRIAFGLAALVAIGLQRPGEILARGGLGGVGGGGGRGGGGGM
                     SRGGGGMSRPSGGMSRPSGGLSRPSGGMSHNINRPSMGSVSRPSAPSRPSFPGGGAQR
                     PSLPGGGNLGGFSPSGGRPSTRPSTPQLPRPQAPGGGIANRPSINPPGDLRPATRPSL
                     PGAGGGSRPSLPGGIADNRPSLPGGNRPSIGNIDRPTTLPGVFPGGGERPNRPDRPVI
                     GGGGDRPLRPDRPIIGGGNNNIINRPGNINIGNDVNIGNNLGNWGINNRPGGDWWSQN
                     HNWHDHWNNGWIHDHHDWYHGCWHGNWGVGWYAPFVWGTAAWGSWGYGPTYYNPYYVE
                     STSAPPYDYSQPVVLNNYVTSDASGGAADATGGAAQAAQQDSPETQQALKLFDDGLAA
                     FKSGGYAQALAKMDAALKLMPTDPVLHEVRALCLFALGRYDESAATLNALLATAPGMD
                     WTSMSALYGNPDDYTAQLRKLEAYVKANRTNAGAIFVLAYHYLVIGQNDEAIKALREV
                     VRLQPKDTTAQRMLGSLAPPEEKPAETTPPPAAPENAPTTDLVGTWRASSGDAVIELT
                     IDDQFNFTWKATPKGKPAVEVKGTVNASSDSLALETKDQGNMVGQVKSGGPDKFTFAM
                     QGLPPGDPGLEFARAG"
     gene            complement(5537..>6268)
                     /locus_tag="VFV87_07280"
     CDS             complement(5537..>6268)
                     /locus_tag="VFV87_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103593.1"
                     /translation="ELIDEEGTNYRGRKELADLFTKFFARFPGAKLAVEIESIRSLGP
                     NLAIEEGTRYIEAVSKETPSRATLRYTAVRTKIEGQWLIASLREFAADPLPTPHERLQ
                     DLAWMVGDWMNEGSDGAVKISFRWSEDENFLLGDYHVTIGGEVRMKSTQRIGWDPLVG
                     KVRSWLFDSDGGFSEGVWTAVEDGWVIKSQSVNPDATTATVTLTITPKDNDHFTIKGT
                     ERIVGDVREPDFEFPIVRRPPVASK"
BASE COUNT         1156 a         1974 c         1934 g         1204 t
ORIGIN      
        1 atcttccagc cattgtcgat catcaggcag aagggctcgc gcaccgccgc ccgatcagcc
       61 gacgcaaagg gctccggcca ggcattgttg cgatgccaat caaccttgct ccgataccac
      121 caactgtgga ccttctcctt agcctggcca ttgcccgcaa ttgcgtcgcc ggccgccacc
      181 cccaggcacg ccatcgtcat ccctgccaca agccagcatc gcatgccgat tcccctcata
      241 aatgcgtagc ggctggcgcg tgccagccca ccgggcgaaa gctatcaccg ctttttcctc
      301 atcggacagc cgcgcggatt caatccacgg cgcacgtgct ccggcggcaa ggtctgcgca
      361 atcggaaaca gcggctgtgt gtcccgacag cggcgcgggt cgacgcaggc attgtgctat
      421 cgggcagcgt gctttacatt tcacgactag cttggcaaat cgccttcctg cctggcgaaa
      481 atgaaggtgc ctgcatgaaa acgcgccccc ggacgaagtt tgtacaccag ctcaaagctc
      541 gccgctgccc aaagtgcggc tcgaagctca acaatcaagt caagcgctgc aagcggtgca
      601 cggccattgt cggcaagccg cggaaatagt gcttgttgcg gatcttcttc ccacccggac
      661 tgccgcgtcc gccagggctc ctgctcattt tcaccgctcg cctgccggta cgcacggcgg
      721 cgaagtagga tgtcggaaga actcatgatt cgcggccagg agattgagct cgatgaaaat
      781 tgcaatgatc ggcactgggt acgtcgggct tgtcaccgga acgtgcctcg ccgacagcgg
      841 caacgaggtc acctgtgtgg atatcgacca ggccaagatc gagcggttgg agcatagcga
      901 aattccaatc tatgaaccgg ggctgagcga gttggtgatc cgcaatgcgg cagcgggccg
      961 gctgaaattc acgaccaaga cagcagatgc tgtaaaaggc gcaaaactga tcttcctggc
     1021 cgtcggcacg cccacgattg gcggacctca gggacgtggc gcagcggacc tgaaattcat
     1081 atggagcgct gtagaggcgg ctgcgccgca cctgcacagc gacgccatcg tcgtcatcaa
     1141 gagcacggtt cccgtcggca caaatgccaa gattcactcg cgtttgagtg gactcactgg
     1201 ccggtcgtgc gacgtggcga gcaatcccga gtttctcaag gaaggggccg cggtcaatga
     1261 ctttcaatat cccgatcgcg tcgtcgtggg tgtgcgcacg cgcgcggccg aggatgccct
     1321 ccgcgagctt tacagcccgt tcctgcggac cgacaaaccg ttcttggcga tgacgcccga
     1381 aagcgctgag ttgacgaagt atgtcgccaa tgccctgctt tcgacgaaga tcagttttat
     1441 caatgaaatg gcgaatctgt gcgagcgact ggggggtgac atcaacgacg tgcgacgcgg
     1501 catcgggcat gatcggcgaa ttggctttgc gtttctgttt cccggtgttg gctatggagg
     1561 gagctgcttt cccaaagacg tgcgggccct ggcgcatatg gctcgcgagg cgggaattga
     1621 gccactcttg ctggacgcgg tcgacgaagt caacagtcgc cagaagcaca tgctaattct
     1681 caagctcgag caacagttcg gaggccatct ggctggcaag cacatcgccc tctgggggct
     1741 ggcattcaag cccaagaccg atgatatccg cgaggcacca gctctcgtac tgatcgatca
     1801 cttgctggag cgtggtgcga aggtcgctgt ccacgaccca gaggcaatgg accacgtccg
     1861 ccagcaatat gcggaaaggc tgaactactc gcacagcgcc ctcgacgtgc tcgagggagc
     1921 agacgcccta gcgatcaata ccgaatgggg cgaattccgc aatccggact tcgctgagat
     1981 gcggcggcgt atgaaacagc ccgtcatcgt cgacggtcgc aacctgtacg atcccgacac
     2041 tatgcgcgcc agcggtttca cctatcattc gatcggccga gtgactgtcc gaccctagcc
     2101 cgggccggtc gcgatctacg gcaagtggcc ggtctcggcg cggggaaact caccggtaag
     2161 cgacttcatg taggccacga ggtcctgctt ctcctgcggc gtgaagttga gtttcttgat
     2221 cttgctggac agctgcggat taggatggcc gcctttgtcg taccattcga caacttcctc
     2281 cagcgttttc tggttgccgt cgtgcatgta cggggcactg tgcaccacgt tgcggatcgt
     2341 tggcgtcttg aacgctcctt tgtccttctc ttccttggtg atttcgtacc ggccgagatc
     2401 cggcttggct gcatccattc caacgcccag gttgtggtac ttttcgtccg tgaaattcgc
     2461 gccgacgtgg caagtcgtgc atttggcctt catcccgaag aacagctcgc ggccccgcct
     2521 ggccgatttg ctgatcgcat gcgcttcgct ggcggccttc agcgcgctgt acttcttgaa
     2581 cgcccctgga ttgtcctcct gaaaagccga cagatcctca agatcgtcgc ggaacgcatg
     2641 cccgaccgcc aggagcggct cataatagtc agcggacgag gggccggtta caagagcccg
     2701 ctcgaatgaa gcaatcgcct tggccacggt gtcgatcgaa attcccttgc tggggaaaat
     2761 aacgtcgaac tgcagcaagt agccgggaat cttcttcagc gtggcgacgc acacctcgtg
     2821 cgtgttgccc atctcgatcg gattgacgat cggccctttg gcctgctctt cgagggtagc
     2881 ggcacggccg tcccaaaact gcaccgagct gaggatgcgg ttgtaactga ccggcgagtt
     2941 gcggccccct tcctgtccgc caatgcccac tccgaatcgc gtgtttttgc catagccggc
     3001 atccggagcg tggcagctcg cgcagctcac ggtgccgtca gccgacagcc gcgaatcgaa
     3061 atagagctgg cgtccaagct cgatcttggc ccgcgtcagg ggattctcaa caggaatctg
     3121 aatagcagac tcgcccatcg ccaggcccag cggcaattcc accgtcagca tctaatggct
     3181 cttgggattg gcgagccagg ccttgatctg gtccgcagtc agcggcccct tcccgggaat
     3241 cccggccgtg aggtcgctgg aaccgagcag gacagtctgc agcaccgcgg gagcgctggc
     3301 atcgccagca aaaacggagg aggcgaacag gccgacagag atcaataccc aagcggacgc
     3361 agacaaagcg cggaacacag gcatggtttc cgagttcagg tcgtggtgac aggccgcgga
     3421 ctgggcggct gcgagttgcc aggtgggatt aagggcggca actccagcga tgtgccgctt
     3481 cagaagtata ggccaggagg taagtgcgcg ggaattgtgc gagatcgcaa agcttcgtga
     3541 aagaggacgg gctcaaaacc cgtcccacga ttgatcttcc gccgcgtgtc agccggcacg
     3601 cgcaaactcc agaccgggat ccccgggggg caggccctgc atggcgaagg tgaacttgtc
     3661 cgggccgccc gatttcacct ggccgaccat gttgccttgg tccttggttt ccagcgccag
     3721 cgagtcgctg gaagcgttca ccgttccctt cacttccacg gcgggcttgc ccttgggggt
     3781 cgccttccac gtaaagttga actggtcgtc gatcgtcagc tcgatcactg cgtcgccgct
     3841 ggacgcccgc cacgtgccga ccaggtcggt ggtgggagca ttctctgggg ccgcgggagg
     3901 cggcgtggtc tcagccggct tctcctcggg cggcgccagc gaaccgagca tccgctgggc
     3961 ggtcgtatcc ttcggctgca agcggacgac ctcgcggaga gccttgatcg cctcatcgtt
     4021 ttgaccgatg accagatagt gataggccag gacgaaaatg gcccctgcat tcgtacggtt
     4081 ggcttttaca taggcttcga gcttccgcaa ctgggccgtg tagtcgtcgg gattcccgta
     4141 tagggcgctc atcgacgtcc agtccatgcc gggcgctgtt gccaagagcg cgttgagcgt
     4201 ggcagcagat tcgtcatagc gtcccagggc gaacaggcac agcgctcgca cttcgtgcag
     4261 caccggatcg gtgggcatga gcttgagcgc ggcatccatt ttggctagcg cttgcgcgta
     4321 gccgccggat ttgaacgccg caagcccatc atcaaagagc ttgagggcct gctgcgtttc
     4381 gggagaatcc tgctgcgctg cttgcgccgc tccgcccgtg gcgtcggctg ctccgccgga
     4441 ggcgtcggac gtgacgtagt tgttcaacac caccggctgc gagtagtcgt agggaggcgc
     4501 tgacgtgctc tccacgtaat aggggttgta gtaggtaggc ccatagcccc atgaacccca
     4561 cgcggccgtg ccccagacaa acggggcgta ccagccgacg ccccaattgc cgtgccagca
     4621 gccgtgatac cagtcgtgat gatcgtgaat ccagccgttg ttccagtggt cgtgccagtt
     4681 gtgattctgc gaccaccaat cgccgccggg gcgattgtta atgccccagt tgccgaggtt
     4741 gtttccgata ttgacatcgt tgccaatatt gatgtttccg gggcggttga tgatgttgtt
     4801 gttcccgccg ccgatgattg ggcggtcggg ccgaaggggc cggtcgcctc cgccgccgat
     4861 aaccggccgg tccgggcgat tggggcgctc gcctccgccg ggaaagacgc cgggcagcgt
     4921 cgtcgggcga tcgatgttgc cgatgcttgg ccgattgccg ccgggaagcg agggccgatt
     4981 gtcggcgatt cctccaggca gcgagggacg actgccgccg ccagctccag gcagcgaagg
     5041 ccgcgttgcc ggccgcaaat cgccaggcgg gttaatcgac gggcgattgg caatgccgcc
     5101 gccgggagcc tgtggccgcg gtagctgtgg cgtgctgggg cgcgtgctcg gccgcccacc
     5161 ggaagggctg aagccgccga gatttccgcc gccagggaga ctggggcgct gggccccgcc
     5221 cccggggaag ctcggccggg aaggcgcgct gggccgcgaa acgctgccca tcgacggacg
     5281 attgatgttg tgcgacattc cgccgctggg gcgggataga ccgccagagg ggcgagacat
     5341 tccgccgctg ggccggctca ttccgccacc gccgcgcgac atacctcccc cgccgccgcg
     5401 tcccccgccg ccgcctacgc cgcccaggcc gccgcgcgcg agaatctcgc ccggccgctg
     5461 caatccgatc gcgaccagcg ccgcgagtcc aaatgcaatt cgaaccttca tcactttgct
     5521 cctcgctggt caggcgttac ttcgaggcca caggtggacg gcgcacgatc ggaaactcaa
     5581 aatcgggctc gcgcacgtcc cccacgatcc gctcggtccc cttgatcgtg aagtggtcgt
     5641 tgtccttggg cgtgatcgtc aacgttaccg tggcggttgt cgcatccggg ttgaccgact
     5701 gcgacttgat cacccagccg tcttcaacag cggtccacac gccctccgag aagccgccgt
     5761 cggaatcgaa cagccacgag cggaccttgc caacgagcgg gtcccagccg atccgctgtg
     5821 tgctcttcat ccgcacttcg ccgccgatcg tgacgtgata gtcgccgagc aggaagttct
     5881 cgtcttccga ccatctgaaa gaaatcttca ccgcgccgtc gctcccctcg ttcatccaat
     5941 cgccgaccat ccaggcgaga tcttgcagcc gctcgtgcgg cgtcggcagc ggatcggcag
     6001 caaactcgcg gagcgaggcg atcagccact ggccctcgat cttcgtccgc acggccgtat
     6061 agcgaagcgt ggcccgcgag ggcgtttctt tggacactgc ttcgatgtag cgcgttcctt
     6121 cctcgatcgc caggttgggg ccaagcgagc gaatcgactc aatctccacc gccagcttcg
     6181 cgccgggaaa acgcgcaaag aacttcgtga acagatctgc cagctctttg cggccgcgat
     6241 agttcgtccc ttcctcgtcg atcaattc
//
LOCUS       DASUGB010000377         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1988008, whole genome shotgun sequence.
ACCESSION   DASUGB010000377 DASUGB010000000
VERSION     DASUGB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988008"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2476
                     /locus_tag="VFV87_07285"
     CDS             <1..2476
                     /locus_tag="VFV87_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103594.1"
                     /translation="NDGKFAVGGSFDQGFAFAGGRFMVVPASRPPSTYAHEAAHMFWG
                     LDEYAGGSTYTAHRGYYDTINVNAEGNPEAGFVQAPSIMALDRISGNPNVPSTFPRTE
                     AYAAHTSSQSSLEMIGWKDSDGDGIFDVLDVPFTLTGSGHYDSSLGRYRFNGESSVRT
                     FANQNPAGLKNDITINRIRVVEYAIDDGPWTVYQAFPDRTYKTALDVSVPMSPGEHTI
                     KIRTHDTRTGVMSPEFIGHTSGPSSAPQSGIGGFVYTDTDGDGTWDDGEAPLAGWDVG
                     LVDAFGGPVILQRRIEPDDSVEGTPLGSGDSKAKLTAVGSDAGRDEVQRLTFSGTGGT
                     VTPLFQGVLGSPIEVIPGVAPTAQDLQGILETIGALSGNVQVSGPAGGPLMVKFGGEL
                     GHTDVPLILQGASTGNAGLSPATTTEGISDDVFALSSQSAPAAQKVFAAMSLQQGGSP
                     VETWTSNSRMLRVDFSDPIAAVSLRAIAGGAASIGRLEAYDAAGKLIERYTTGVLTAG
                     KQELMTIQRPTPDIAYVLARSHLGTEVILDTLTWGPNSSTTTDAQGAYSLPNLDSGLY
                     RVKLTPPEGHVVTSPSGGVHTVQFTAGQNASDLNFGLHRLDFSNPWHNLVLAENVNND
                     PGGLVNVLDLLAIVNWITAQGGIAQLPPAGDTQANGYVDVDNNGICNVLDVLAVVNYI
                     TVHYSTGTGGGAGENLLGRSESLAGSIPGQAASPQGEGETLSPIPAAAYYAVIPWHYL
                     EAKGDDELSCAAEEAGHSSPQASPAPPAAPPLPAFEASAAPTAARLTPPIEDPLHERL
                     DESIALLAADIAQSRSTRSRLARRYG"
BASE COUNT          474 a          843 c          829 g          417 t
ORIGIN      
        1 gaacgacggg aagttcgccg tggggggctc gttcgaccag ggctttgcct tcgcaggcgg
       61 gcggttcatg gtcgtccccg ccagcaggcc gccaagcacg tatgcccacg aggcggccca
      121 catgttttgg ggcctcgatg agtacgccgg cggcagcacg tacacggcgc atcgcggcta
      181 ctacgacacg ataaacgtca acgcggaagg caatccagag gcgggcttcg tgcaggcgcc
      241 cagcatcatg gccctcgatc ggatcagcgg caatccgaac gtgccctcca ccttcccgcg
      301 gaccgaggcc tatgccgccc ataccagctc gcagtcgtcg ctggaaatga tcggctggaa
      361 agattcggac ggggacggga tattcgacgt cctcgacgtg cccttcacgc tcaccggcag
      421 cggccattac gactcgtcgc tgggccgcta ccgattcaac ggcgagagca gcgtgcggac
      481 ctttgccaac cagaacccgg ccggcctgaa gaacgacatt acgatcaatc gcatccgggt
      541 cgtcgagtac gccatcgacg atggtccgtg gaccgtctat caggcatttc ccgaccggac
      601 ttacaagacc gccctggacg ttagcgttcc gatgtcgccg ggcgagcaca ccatcaaaat
      661 ccgcacgcac gacacacgga cgggcgtcat gtcgcccgaa ttcatcggcc ataccagcgg
      721 gccgagcagc gcgccgcagt ccggaatcgg cggtttcgtc tataccgaca ccgatggcga
      781 cggcacgtgg gacgacggcg aagcgccgct ggctggttgg gacgtgggcc tggtagatgc
      841 cttcggcggg ccggtgatcc tccagcggcg catcgagccg gacgattccg tggagggaac
      901 gccgctggga tcgggcgatt cgaaggcgaa gctcactgcc gtgggcagcg atgcgggccg
      961 ggacgaagtg cagcgcctga ctttcagcgg gacgggcggc acggtgaccc ccttgttcca
     1021 gggcgttctc ggctcgccga ttgaggtcat tccgggcgtg gcgccgaccg cgcaagatct
     1081 gcagggaatc ctggaaacga ttggggccct cagcggcaac gtgcaggtca gcggacctgc
     1141 cggcgggccg ctgatggtga aattcggcgg cgagctcgga cataccgacg ttcccctgat
     1201 tctccagggc gcttcgaccg gcaacgcggg actcagtcca gcgactacca ccgagggcat
     1261 cagcgacgac gtgtttgcgc tttccagcca atcggccccc gcggcgcaga aagttttcgc
     1321 ggccatgagc ctccagcagg gcggctcgcc ggtcgaaacc tggaccagca actcgcggat
     1381 gctgcgggtg gatttttccg acccgatcgc tgccgtcagc ctgcgggcca ttgccggcgg
     1441 ggccgccagc atcggccgcc tcgaagcgta cgatgcggcg ggcaagctca tcgaacgtta
     1501 cacgaccggc gtgctgaccg cgggcaagca agagttgatg acgatccagc ggccgactcc
     1561 agatatcgcc tatgtgctcg cccgctcgca tctgggcacc gaggtaatcc tcgacacgtt
     1621 gacctggggg ccgaattctt cgaccacgac tgacgcccaa ggagcgtact ccctgccgaa
     1681 cctggatagc ggcctgtatc gagtcaagct gacgcctccc gagggtcacg tcgtgacctc
     1741 gccgtccggc ggagtccaca ccgttcagtt tacggctggg caaaatgcga gcgatctgaa
     1801 tttcggcctg caccgcctcg acttcagcaa tccctggcac aacctggtcc tggcagaaaa
     1861 cgtgaacaac gatccgggcg ggctcgtgaa cgtgctcgac ttgctggcca tcgtgaactg
     1921 gatcactgct cagggcggca tcgctcaact tccgcccgcc ggggatacgc aggccaacgg
     1981 ctatgtcgat gtcgataaca acggcatctg caacgtgttg gacgtgctgg ccgtggtcaa
     2041 ctacatcacg gttcactact ccacgggcac ggggggcgga gcgggcgaaa acctgctcgg
     2101 cagaagcgag tcgctcgccg gcagcatccc gggtcaagcc gcttcgccgc aaggcgaagg
     2161 agaaacgctt tcgccgatcc ctgctgccgc gtactacgcc gtgattccct ggcattactt
     2221 ggaggctaag ggggacgacg aactgtcctg tgccgccgag gaggcagggc attcctcgcc
     2281 ccaggcgtcg ccagcgccgc cagcagcgcc gccgctgcca gcgttcgagg cgagtgcagc
     2341 gccgacagct gcgcgactga caccgcccat cgaagacccc ctgcacgagc gactggacga
     2401 gtcgattgct ctcctggccg ccgatattgc ccaatcgcga agcactcgca gccgcctggc
     2461 ccgccgctac gggtaatgat gaatcaagcc cctcgcgggg agcaggcgat ttggcagttc
     2521 ggtcaatcgc gagcggccgg tgcggcaagg ctcggctccg gct
//
LOCUS       DASUGB010000378         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1015893, whole genome shotgun sequence.
ACCESSION   DASUGB010000378 DASUGB010000000
VERSION     DASUGB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015893"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFV87_07290"
     CDS             <1..248
                     /locus_tag="VFV87_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5103595.1"
                     /translation="GIVLFSGVVFFQLVNLPVEFNASRRAKQLLVETGIVSGHEMGPV
                     NSVLNAAALTYVAGTLQSVLTLLYYILHYTSRQRGRS"
     gene            complement(271..1761)
                     /locus_tag="VFV87_07295"
     CDS             complement(271..1761)
                     /locus_tag="VFV87_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103596.1"
                     /translation="MTTTFRQPEVLEPPVIVSPPEEIAAEAVEDLDSPQVLAESVEQD
                     ILAEAVESPELVAAEAIGEVSFPAPEPKPIMGDGKAPWPKSWPFIAWHGFKLAAEWLF
                     GLASVIGCLAVLAAIPILNFISLGYLLEASGRVAKSGRIRDGFIDTAKFARIGSLVAG
                     TWLWLWVPRFVSTLATDAWLIDPNGAPARAWRVGLIVTTVLVIGHILLAWYSGGRLRH
                     FFWPFLAPFQLGAKLLFGGIVGPIFRPLVTALWPAFAEDLYNTRPLSEWFPPAILAAG
                     IRRGGMYAQARDAVWDFIVSLRLPQYFWLGLRGFVGALAWLIVPSLMLVAGTSGPPVL
                     ILVGYLGAALMAFVLIYLPFLQTHFACQNRFTAIFEWSAVRQQFRRAPIAYWLALTIT
                     LLFALPLYLLKIEPPPRELWWTLTIFFILFIYPARLLTGWAVGRAMHHAQPRFWLFRW
                     MSRLAAIPVVLFYLLILFFTQYTSYLGPASLLEQHAFLLPVPFLAL"
     gene            1873..>3296
                     /locus_tag="VFV87_07300"
     CDS             1873..>3296
                     /locus_tag="VFV87_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103597.1"
                     /translation="MWAKNALFIVVCLVGAGAVANNLLRRDRIDRPDTFEPGRFEATS
                     AAGAAETGGTYKSTLDKLNAQFHEHWQKKGLEVAPRADDLTIARRMSLALVGTVPSLE
                     EIRALEEVEPQQRREFWTSHLLEDPRFGDYWAERLARAYAPPGNSPFIVFRRRRFTMW
                     LADNIRENRPYDEMVRQMISETGVWTGKPAANWVTATSDPNKENQPDPVLLAGRTARA
                     FLGMRIDCLQCHDDKLGNIMVGSAEKIHSGTQADFHQLAAFFGQTRVSLTGVQETNKE
                     YKVKYLNGEEEETVPAKVPFSDELLNDDGSRREQLARWVTHPENKPFARATVNRVWAL
                     MFGKPLVEPIDDIPLVGRYPPGLQLLADDFVAHHYDLQRLIRLIAASDAFQRDSRAEF
                     EITDQHENELAVFPLTRLRPEQMAGSLVQASWLTTINGQAHVFVQIGKFTQTNNFVER
                     YGDLGQDEFTDRAGTIPQRLLMMNG"
BASE COUNT          683 a         1044 c         1055 g          514 t
ORIGIN      
        1 ccggcatcgt gctcttcagc ggagtcgtgt ttttccagct cgtcaatctg ccggtcgaat
       61 tcaacgccag ccgccgggcc aagcagctcc tggtggaaac gggcatcgtc agcggccacg
      121 aaatgggccc cgtcaacagc gtcctcaacg ccgcggccct cacctacgtc gccggcacgc
      181 tgcaatcagt tctcacgctg ctctattaca tccttcacta caccagccgc cagcggggac
      241 gaagctgacc cacacggcgg atggggcaaa ttacagtgcc aagaacggca ccggcaggag
      301 gaaggcgtgc tgttcgagca ggctggcggg gccgaggtag gaggtgtact gcgtgaagaa
      361 caggatcagc agatagaaca gcacgacggg aatcgccgcc agccgggaca tccagcggaa
      421 gagccagaat cgcggctggg cgtggtgcat cgcccggccg acggcccagc cggtgagcag
      481 ccgagcggga tagataaaga ggatgaaaaa gatcgtcagc gtccaccaca gctcgcgcgg
      541 cggcggctcg atcttgagca gatacagcgg cagcgcaaag agcagcgtga tcgtgagggc
      601 cagccaatag gcgatcgggg cgcggcggaa ctgctggcgg acggcgctcc actcgaagat
      661 cgccgtgaag cggttctgac aggcgaaatg cgtctgcaaa aacggcaggt agatcagcac
      721 gaaggccatc agcgcagcgc cgagatagcc gacgagtatg agcactggcg gtcccgatgt
      781 cccggcgact agcatcaggc tcggcacgat cagccaagcg agagcgccca caaagccgcg
      841 caggccgagc cagaaatact gcggcaggcg gagtgaaacg ataaaatccc agaccgcatc
      901 gcgggcctgg gcgtacatgc cgccgcggcg gatccccgcc gcgaggattg cgggcgggaa
      961 ccactccgag agcggcctag tgttgtagag gtcttcggca aacgcgggcc agagcgccgt
     1021 gacgagcgga cggaagatcg gcccgacgat tccgccaaag agcagcttcg ccccaagctg
     1081 gaacggcgcg agaaacggcc agaagaaatg ccgcagccgc ccgccgctgt accaggcaag
     1141 cagaatgtgt ccgatgacga ggaccgtcgt cacgatcagg ccgacgcgcc acgccctggc
     1201 gggtgcgccg tttggatcga tcagccaggc gtcggtggcc agcgtggaaa cgaagcgcgg
     1261 cacccagagc cacagccacg tgccggccac caggctgccg atgcgggcga atttcgctgt
     1321 atcgataaac ccgtcgcgaa tccggccgct tttggcgacc cggccgctgg cttccagcag
     1381 gtagcccagg ctgatgaagt tcaggattgg aatcgccgcc agcacggcca ggcagccgat
     1441 gacgctcgcc aggccgaaaa gccactctgc tgccagcttg aagccatgcc aggcgatgaa
     1501 gggccagctt tttggccacg gggccttgcc gtcacccatg atcggcttgg gctctggtgc
     1561 tgggaagctc acttccccga tcgcctccgc tgctaccaat tcgggcgatt cgacggcttc
     1621 ggccaggatg tcttgctcca ccgattcggc cagcacctgg ggcgaatcga gatcctccac
     1681 cgcctcagcc gcgatttcct ccggcgggga cacaatcacc ggcggctcaa ggacttcggg
     1741 ctggcgaaac gtcgtcgtca cgggcgaaac tagaggtgca gcaggcgggt aaaatgaatg
     1801 gcagccagcg gccagccgac cgttatacct atagccttcg gccggccgtt tcaagaaaat
     1861 ccagcttacg ccatgtgggc caagaacgca ctatttatcg tcgtttgcct ggttggcgcc
     1921 ggagcggtcg ccaacaacct gctgcggcgg gaccgaatcg accgcccgga tacgtttgag
     1981 ccggggagat ttgaggcgac ctctgccgcc ggtgctgccg aaaccggcgg gacgtacaag
     2041 tccacactgg acaagctcaa cgcgcaattc cacgaacact ggcagaagaa ggggctcgaa
     2101 gtcgccccgc gggccgacga tctgacgatt gcccgccgca tgtcgctggc gctcgtcggc
     2161 acggtgccgt cgctggaaga gattcgcgcc ctcgaagagg tcgagccgca gcagcgtcgc
     2221 gaattctgga cgagccacct gctggaggac ccgcgcttcg gcgactattg ggccgagcgc
     2281 ttggcacggg cgtatgcccc gccggggaac agcccgttca tcgtgtttcg gcggcggcgg
     2341 ttcacgatgt ggctggccga caacattcgc gagaaccggc cctatgacga gatggtacgg
     2401 cagatgatct ccgagacggg ggtctggacc ggcaagcccg cggcaaactg ggtcacggcg
     2461 acatccgatc cgaataaaga gaatcagccc gatccagtgc tgctcgccgg ccgcacggcc
     2521 cgtgcattcc ttggcatgcg gattgactgt ctgcagtgtc acgacgacaa actcggcaac
     2581 atcatggtcg gatcggccga aaagattcac agtggcacgc aggccgattt ccaccagctc
     2641 gcggccttct ttgggcaaac gcgtgtttcg ctgaccggtg ttcaggaaac caacaaggaa
     2701 tacaaggtca aatacctcaa cggggaagaa gaggaaacgg tcccggccaa ggttcctttt
     2761 tccgacgagc tgctcaacga cgacggctcg cggcgcgagc agctcgcccg ctgggtcacc
     2821 cacccggaaa acaagccgtt tgcccgcgcc acggtgaacc gcgtttgggc cttgatgttc
     2881 ggcaagccgc tcgttgagcc gatcgacgat attccgctcg tcggccgcta tccgccgggg
     2941 ctgcagctcc tggccgacga tttcgtcgcc catcactacg acctgcagcg gctcatccgc
     3001 ttgatcgccg ccagcgacgc cttccagcgc gacagccgcg ctgagtttga gatcaccgac
     3061 cagcacgaga acgagctggc cgtatttccg ctgacgcgct tgcggcccga gcagatggcc
     3121 ggttcgctcg tgcaggcttc gtggctgacg acgatcaacg gtcaggcgca cgtctttgtc
     3181 cagatcggca agttcacgca gaccaacaac ttcgtggaac gttacggcga cttgggccag
     3241 gatgaattta cggaccgcgc cggcaccatc ccgcagcggc tcctcatgat gaacgg
//
LOCUS       DASUGB010000379         4779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_42369,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000379 DASUGB010000000
VERSION     DASUGB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42369"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1088
                     /locus_tag="VFV87_07305"
     CDS             111..1088
                     /locus_tag="VFV87_07305"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5103598.1"
                     /translation="MPKAICLNETGGPEVMKWEEVAVGEPGAGEARIRHTAIGVNFID
                     TYHRSGLYPLLLPSGLGSEGAGIVEAVGPGVTHLRAGDHVAYAGGPVGSYCEARVMPA
                     DRLLRLPTGISDPQAAAMMLKGMTVQYLIRRTFRVEPGQTVLFHAAAGGVGLIACQWL
                     KALGATVIGTVGSKEKAQLAQAHGCDHTINYSDENFAARVREITGGAGVPIVYDSVGK
                     STFEGSLDCLRPRGLLVSFGNASGPVPPFPLSLLTQKGSLYVTRPTLATYTATRDELE
                     ATAQELFDVVQSGKVKIEINHTYRLEDAEQVHRDLEARKTTGSVVMLPG"
     gene            1118..2635
                     /locus_tag="VFV87_07310"
     CDS             1118..2635
                     /locus_tag="VFV87_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103599.1"
                     /translation="MTRTWPIAMSLVLSALLAQAAEQPKTLKSGVQLLVDDYLIGESQ
                     GVERKVVQPQRFLDGPIVTGALEHQDFQPFLTVLHDETNRTGKPFRMWYNVDVVDDPR
                     DGEWFGKTGYLESADGIHWPGPYRRLNSIAEDGRVRFGASVLDEGPRHPTPAERYKML
                     YWDVGKFIGPRVAFSSDGLEWAVHNGGQPILPKTPSDDIWTAGYDPLRQRYFLIGKQF
                     GPHTWTNAEGEKLTLPIRRYFTSFSPDFKTWSDPEGLVYSPDEKDSGVTQWYGSAGYQ
                     VRGDLIIGFLRELRDDRSPAGVPEEAIKANNGGGANLGANLLGERGGSGMGYTVLTWT
                     RDGQTWHRDRYTDKFFEPDPKVGAWDHAMAWVGSAAPVGDELYLYYAGYRWGHKYHHS
                     VDRQFGLVKIPRDRYVARQAGAEGGKLTTPLVTLDGESLALNVAAKEGEVRVQVTNDD
                     GTPIAGFRFEDCQPIAADSLAAPVAWSESLAKLRGQAVRLEFSLKNASLFAFELR"
     gene            2663..4444
                     /locus_tag="VFV87_07315"
     CDS             2663..4444
                     /locus_tag="VFV87_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5103600.1"
                     /translation="MTPLRMVLALLVLAACSSVVQSSRAADKPNVLIVMTDDQGLGDF
                     SFMGNPVLKTPHLDAFAREAVRFTDFHVCPMCSPTRGQLLTGLVALRNGATSVTAGRT
                     FLRPGIPTMADAFAGAGYRTGLFGKWHLGDFYPHRPIDKGFQESIYHLGWGQLHSTPE
                     FDWPLIDGRYFHNGVEKRYQGHCTDFWFDQAMAWMSACKKEGQPFFCYLPTNAPHAPH
                     IDLDEFVQPYEEKGPAGFFGMIAHIDRRFGDLDEFLAREGLRDNTIVIFMTDNGGTAG
                     VRIFNAGLRGNKTMYYDGGHRVPCWFRWPDGKLGEPRDIALPTQNTDLLPTLCELCGI
                     PQPKGNAADEPYRGTSLAAVLRGGEPARSLTDRKLVVQYGQIPQKFESCVIWGKWRLV
                     KGEELYDTEADRAQVANVAEKHPDVVQAMRDYYDEWWSGLTPILNDFVPISIGAGEQP
                     VVELTSGDWESIYADNTGHVREAVGGPTGGHWHIQVVQPGDYVFTLRRWPEQTKAALG
                     AKYEPSTRSPANRPNVKTVGFPTIARARLEIADQKAEVAADAKEAGVTITVADLSKGR
                     TTLKAWFADAEGKDLCGAFFVTVVRKK"
     gene            complement(4445..>4779)
                     /locus_tag="VFV87_07320"
     CDS             complement(4445..>4779)
                     /locus_tag="VFV87_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5103601.1"
                     /translation="TFAALANHTPSTDPKWDGTNIWPVLADNKPLAPRPLYWTAPGFR
                     ASAVRLGDWKLIATGSGDAQKVELFDLASDPNETTDLAAKMPDKVAQLKAKLAELAAR
                     DRDAVAKD"
BASE COUNT          869 a         1444 c         1633 g          833 t
ORIGIN      
        1 tcggaaagtt gcgccccctg gttgttgttg ccacctgaat ctccaacggc gctttgggga
       61 cttgtgcaaa gcgggcaagc cgcggtaatt acgttgcagg gagaaatggc atgcccaagg
      121 caatttgctt aaatgagacc ggggggccgg aggtcatgaa gtgggaggag gtcgccgtcg
      181 gcgaaccggg ggcgggagag gcacggattc gacacacggc gatcggcgtc aactttatcg
      241 acacgtatca ccgctcgggg ctctatccgc tgctgctgcc gagcggcctg gggagcgaag
      301 gggcgggaat cgtcgaagcg gtcggcccgg gcgtgacgca cctgcgagcc ggcgaccatg
      361 tggcctacgc cggcgggccg gtcggctcgt attgcgaagc gcgggtcatg ccggccgatc
      421 gcctgctccg cctgcccacg ggcatcagcg atccgcaagc cgcggcgatg atgctcaaag
      481 ggatgacggt gcagtacctg atccgccgca cattccgcgt cgagcccggc cagaccgtgc
      541 tcttccacgc cgccgcggga ggcgtcggtc tgatcgcctg ccagtggctc aaggcgctcg
      601 gcgcgacggt catcggcacg gtcggctcga aggagaaggc gcagctcgcc caggcccacg
      661 gctgcgacca cacgatcaat tacagcgacg aaaacttcgc cgcgcgcgtc cgcgagatca
      721 cgggcggcgc gggcgtgccc attgtttatg attcggtcgg caagagcaca ttcgagggct
      781 cgctcgattg cctgcgcccg cgcgggctgc tcgtcagctt cggcaacgcc agcggcccgg
      841 tgccgccgtt tccgctcagc ctgctgacgc aaaagggctc gctgtatgtc acccggccta
      901 cgctggcgac ctatactgca acccgcgacg agctggaagc gaccgcccag gagctgttcg
      961 acgtcgtcca atccggcaaa gtgaaaatcg agatcaacca cacctatagg ctagaggacg
     1021 ccgagcaggt ccaccgcgac ctggaagccc gcaaaaccac cggctcggtc gtgatgctgc
     1081 cgggttgagt tctacgccac gcgaaaggag caattcgatg acccgcacct ggccaatcgc
     1141 gatgtcgctg gtcctgagcg ccttgctcgc gcaagccgca gagcagccca aaacgctgaa
     1201 aagcggcgtg caactgctgg tggacgacta cctcatcggc gagagccagg gggtcgagcg
     1261 gaaggtcgtc cagccgcagc gattcctgga tgggccgatc gtgaccggcg cgctcgagca
     1321 tcaagacttt cagccgttcc tgactgtcct tcacgatgaa acgaatcgga ccggcaagcc
     1381 gttccggatg tggtacaacg tcgatgtcgt cgatgatccc cgcgacggcg agtggttcgg
     1441 gaagaccggc tacctggaat cggccgacgg tattcattgg ccgggaccgt atcggcggct
     1501 gaactcgatt gccgaggacg gccgcgtgcg gtttggggcg agcgtgctcg acgaagggcc
     1561 gcggcatccg acgccggccg agcggtacaa gatgctgtac tgggatgtcg gcaagttcat
     1621 cggcccgcgt gtcgcctttt catctgacgg tctggaatgg gccgtccaca atggcggcca
     1681 gccgatcttg cccaagacgc ccagcgacga catctggacc gccggctacg atccgctccg
     1741 ccagcggtat ttcctgatcg gtaagcagtt tggcccgcac acctggacca atgccgaggg
     1801 ggagaagctc acgctgccga tccggcgata cttcaccagt ttcagcccgg acttcaagac
     1861 ctggagcgat ccggaggggc tggtctattc gcccgacgag aaggattcgg gcgtcacgca
     1921 gtggtacggc tcggcggggt atcaggtccg cggggatttg atcatcggct ttttgcggga
     1981 gctgcgcgac gatcggtccc ccgccggagt tcccgaggag gcgatcaagg ccaacaacgg
     2041 cggaggggcg aacctggggg cgaatttgct gggcgagcgc ggcggctccg gcatgggcta
     2101 tacggtgctc acgtggaccc gcgacggcca gacctggcat cgcgaccggt acacggacaa
     2161 gttcttcgag ccggacccca aagtgggcgc gtgggaccac gcgatggcct gggtcggcag
     2221 cgcggcgccg gtcggcgatg agttgtatct ctactacgcc ggctaccgct ggggacacaa
     2281 gtatcatcat tcggtcgatc ggcagtttgg cctggtgaag ataccgcgcg accggtacgt
     2341 cgcccggcag gccggcgcgg agggcgggaa gctgaccacg ccgctggtga cgctggatgg
     2401 agaatcgctg gcgctcaatg tcgccgcgaa agaaggggag gtgcgcgtac aagtgaccaa
     2461 cgacgacggg acccccattg ccggctttcg cttcgaggac tgccagccaa tcgcggctga
     2521 ttcactggcc gccccggtcg cgtggagcga atcgctcgcg aaactccgcg ggcaagcggt
     2581 gcggctggaa ttctcactga agaatgccag cttgtttgcc ttcgagcttc gatagaactc
     2641 accaacagac gagctaacca agatgacacc tctccgaatg gttctcgcgc ttctggtcct
     2701 ggcggcctgc tcatcggttg tccaaagcag ccgggcagcc gacaagccga acgttctgat
     2761 cgtgatgacc gacgatcaag gtctgggcga tttctcgttc atgggcaatc cggtgctgaa
     2821 gacgccgcat ctggatgcct tcgcccgcga ggcggtgcgg ttcactgatt ttcacgtctg
     2881 tccgatgtgc agtccgacgc gggggcaatt gctgaccggc ctggtggcgc tgcggaatgg
     2941 ggcgacctcc gtgacggctg gccgcacgtt tttgcggccc ggcattccga cgatggccga
     3001 tgccttcgcc ggcgcgggct accgcacggg cctcttcggc aagtggcatc tgggcgactt
     3061 ttacccgcac cggccgatcg acaaaggttt tcaggagagt atctatcatc tcggctgggg
     3121 gcagcttcat tcgacgccgg agttcgactg gccactcatc gacgggcggt actttcacaa
     3181 cggcgttgaa aagcgctatc aggggcattg cactgatttc tggttcgatc aggcgatggc
     3241 ctggatgagc gcctgcaaga aggaaggcca gccgttcttc tgctacctgc cgacgaacgc
     3301 tccgcacgcg ccgcacatcg acctcgatga attcgtgcag ccgtacgaag agaagggccc
     3361 ggccggtttc ttcgggatga tcgctcacat cgatcggcgg ttcggcgatc tggacgagtt
     3421 tctggcgcgc gagggcctgc gcgacaacac gatcgtgatc ttcatgaccg ataacggcgg
     3481 cacggcgggg gtccgcatct tcaatgcggg gctgcgcggg aacaaaacga tgtactacga
     3541 cggcggccat cgcgtgccgt gctggtttcg ctggccggat ggcaagctgg gcgagccgcg
     3601 cgatatcgca ctcccgacgc agaacaccga cctccttccc acgctctgcg agctgtgcgg
     3661 aattccgcag ccgaaaggaa acgcggccga tgagccatat cgcggcacga gcctcgcggc
     3721 ggtcctgcgc ggcggtgaac cggctagatc gctgaccgat cgcaagctcg tcgtgcagta
     3781 cggtcagatt ccgcagaaat tcgagtcgtg cgttatttgg ggcaagtggc ggctggtgaa
     3841 gggggaggag ctgtacgaca ccgaagccga ccgcgcacag gtggcgaacg tcgccgaaaa
     3901 acatccggac gtcgtccagg cgatgcgcga ctattacgac gagtggtgga gtggcctgac
     3961 gccaattctg aacgactttg tcccgatcag catcggcgcc ggcgagcagc cggtggtcga
     4021 gctgacgagc ggcgactggg agagcatcta cgcggacaac acgggacacg tgcgcgaggc
     4081 ggtcggcggt ccgaccggcg ggcactggca cattcaggtc gtgcagccgg gcgactacgt
     4141 gttcacgctc cgccgctggc cggagcagac gaaggcggcg ctcggtgcga agtacgagcc
     4201 atcaacacgc tcgcccgcca atcggccgaa cgtgaagacc gtcggctttc cgacgatcgc
     4261 gcgggcccgg ctcgagatcg cggaccagaa agccgaagtc gcagcagatg cgaaggaggc
     4321 gggcgtgacg atcaccgttg cggatctgtc caaggggcgg actacgctca aggcctggtt
     4381 tgcggatgcg gaggggaaag atctttgtgg ggcgttcttt gtgacggtgg ttcggaagaa
     4441 atagtcagtc cttggcaacg gcgtcgcggt cgcgggcggc cagctctgcg agtttcgctt
     4501 taagctgggc gactttgtcc ggcattttcg ccgcgaggtc ggttgtttcg ttgggatcgc
     4561 tggccagatc gaacagctcg actttttggg catcgccgct gccggtggcg atcagcttcc
     4621 aatcccccag gcgcacggcg ctggcgcgga agcccggcgc cgtccagtag agcgggcgcg
     4681 gagcgagcgg cttgttgtcg gcgaggacgg gccagatgtt cgtgccgtcc catttcggat
     4741 cggtggaggg cgtatgattc gcgagcgcgg caaacgtcg
//
LOCUS       DASUGB010000380         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1571915, whole genome shotgun sequence.
ACCESSION   DASUGB010000380 DASUGB010000000
VERSION     DASUGB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571915"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1343)
                     /locus_tag="VFV87_07325"
     CDS             complement(312..1343)
                     /locus_tag="VFV87_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5103602.1"
                     /translation="MINPSASVRSWRLAAGEGSAGSSDNLPPPAATAARRDAELLDAY
                     SQAVIGVVGRVGPAVLSIGSHPADDQPGSGSGFVITPDGFAVTNSHVVRGRERLRATT
                     EEGDVLDARLVGDDPATDLALLQVAARDLPHAELGDSDGLQVGQLVIAMGNPFGFRST
                     VSTGVASALGRAMRSEHGRLIENIVQHTAPLNPGNSGGPLVDSRGRVVGINTAIIALA
                     QGLGFAVPATTAQWVVGELMSHHRVRRLSLGVTVTIAAIPRRLVRELDLLGDQAIEVV
                     EVLSPGPAAAAGVLAGDWIVTAGGRITSGADDLHRVLATLKAEREIPLQIVRGEGLIE
                     LAVTPRWSP"
     gene            1661..2092
                     /locus_tag="VFV87_07330"
     CDS             1661..2092
                     /locus_tag="VFV87_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103603.1"
                     /translation="MSPFTSAIGAMVVALGLSPQEDVAREQVDLIEVNHFYDEQGRLV
                     FDQVIFYDWSADHSRYMVRAWRLVKNPAQLPQRDWKDGGYAAVWQDGEVLRHVCAESM
                     RETWTQYDPELVEREFLPKEQRKELRVIKVERPAPQVASSP"
     gene            complement(2118..>2631)
                     /locus_tag="VFV87_07335"
     CDS             complement(2118..>2631)
                     /locus_tag="VFV87_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103604.1"
                     /translation="FKTLSEPFITTFASRGPGEKLLVSVNGQDQPVSQPGAIGPDAGA
                     AGFTVGSREDIPPGAQCWAGDLAEILVYDRELSADELQEAGAYLTTKYALSADYPRRS
                     PLPFDAAQTAEKDRAFITDLYYAAFSRPPTDDELAADLAYIQSAADRRQGLEDVSWAV
                     LNAKEFLFQH"
BASE COUNT          477 a          862 c          831 g          461 t
ORIGIN      
        1 aaacaaattc cgcccagaat ccgcccgctc cccaacttac gcctcgcaca ctcgccctaa
       61 ccccttcccc gccaagcact tccgccccca ccccgcgccc taagtttttc tcgcccccgc
      121 ggatgaaaag gacacgtctg gtccattctg agcacacgaa acaggatgtg tacaacgcaa
      181 tttcgccgga ctgcgggccc gcgaagctca tttcgccgcg tggaatccca ccgcgctctc
      241 cgccggacgc tgcgcgcagc ccccgcccgg tgcaagactt gaaaggggcc ggcctcgctg
      301 acgcgtcgcg cttacggtga ccagcggggc gtgacggcca attcgatcag tccctcgccg
      361 cggacgatct gcaggggaat ttcgcgctcg gctttgagcg tggccagaac gcggtgcaag
      421 tcgtcggcgc ccgatgtgat tcgcccgccc gcagttacga tccagtcgcc ggccagcacg
      481 ccggccgcag cagcaggacc agggctcagc acttccacga cctcaatcgc ctgatcgccc
      541 agcaggtcga gctcccgcac cagcctccgc gggatggccg caatcgtcac cgtgactccc
      601 agcgacagcc gccgcacgcg atggtggctc atcagctcgc cgacgaccca ctgggctgtc
      661 gtcgcaggca cagcgaagcc gagaccctgc gccagggcga tgatcgccgt attgatgccg
      721 acgacgcgcc cgcgcgaatc gacgagcggc ccgccgctgt tgcccggatt cagcggggcc
      781 gtgtgctgca cgatgttctc gatcagccgg ccgtgctcgc tccgcatggc ccggccgagc
      841 gcactggcga cgccggtcga aaccgtcgag cgaaagccaa acgggttgcc catcgcgatg
      901 acgagctgcc cgacttgcaa accgtcgctg tcgcccagct ctgcgtgcgg caggtcgcgg
      961 gccgcgactt gcaagagggc cagatcggtc gccggatcgt cgccaacgag gcgggcgtcc
     1021 aggacgtcac cttcttcggt tgtcgcccgc aaacgctctc ggccgcggac gacgtggctg
     1081 ttggtcaccg caaagccgtc cggcgtaatg acaaatccgg agccgctgcc gggctggtcg
     1141 tcggctggat ggcttccgat ggacagcacc gccggtccga cgcggcccac gacgccgatg
     1201 accgcttgcg aataggcgtc caatagttcc gcatcgcgcc gcgccgccgt ggctgctggc
     1261 ggcggcagat tgtccgacga acctgccgaa ccttcgccag ccgccaaccg ccagctccga
     1321 actgaagcag atggattgat catgtttcgt gccctcaagc ccaaaaagtc agctaccgaa
     1381 ttctacgcat cgtgcgggaa aggcaagata agggcggtca ttaccccggt atcgccgtca
     1441 caattgccac atctccgcgc ttatttacgt gagagggcaa gcggctgcga gcccggcacg
     1501 atgattgctt tcgcgttttt gcgtggtttg ttcggaatcg agaggcggct gtcaagattt
     1561 cgtcttgacg cggtttgtga cttccgatac tagtcgataa cgcgttagcc ccggggtctc
     1621 gccgcaaaaa gccctctcct tagggaaaag gaggtgactg gtgagtccct ttacatccgc
     1681 gatcggcgct atggttgttg ccctgggtct ctctccgcag gaggacgtgg cccgggaaca
     1741 ggtggacctc atcgaggtca accacttcta cgacgagcag gggcgcctgg tcttcgacca
     1801 ggtcattttt tacgactggt cggctgacca cagccgctat atggtccgcg cctggcgact
     1861 ggtgaagaat ccggcccagc tgccgcagcg cgactggaag gacggcggct atgcggccgt
     1921 gtggcaggat ggcgaggtgc tgcgacacgt ttgcgccgag tcgatgcgcg aaacgtggac
     1981 gcaatacgac cccgaactgg tcgaacgcga gttcctgccc aaagagcagc ggaaggagtt
     2041 gcgggtcatc aaggtcgaac gcccggcgcc gcaagtggcc agttcgccat agctctgcgc
     2101 caagcgagtc cgcgatccta gtgctggaac aggaattcct tggcgttcag cacggcccat
     2161 gagacgtctt ccagaccctg gcggcggtcg gcggcggatt ggatgtaagc caggtcagcg
     2221 gccagttcgt catctgtcgg agggcggctg aaggcggcgt agtacagatc ggtgatgaag
     2281 gcccggtctt tctctgcggt ttgcgcggcg tcgaaaggca ggggcgagcg acgcgggtaa
     2341 tcggcagaca gagcgtattt tgtcgtcagg taagcgccgg cctcctgcaa ttcatcagcc
     2401 gacagctctc ggtcatagac cagaatttcg gccaggtcac cggcccagca ctgcgctccc
     2461 ggcgggatat cctcgcggct gccgaccgtg aagccggcgg cgccggcatc gggaccaatg
     2521 gccccgggct ggctgacagg ctgatcctgg ccgttgacgc taacaagcag cttttcgccg
     2581 ggaccgcgag atgcgaacgt ggtgataaat ggctcgctta gggtcttgaa g
//
LOCUS       DASUGB010000381         4860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1015990, whole genome shotgun sequence.
ACCESSION   DASUGB010000381 DASUGB010000000
VERSION     DASUGB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015990"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2371
                     /locus_tag="VFV87_07340"
     CDS             <1..2371
                     /locus_tag="VFV87_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5103605.1"
                     /translation="FLFLLNFRTTFITLTAIPLSIVVTGLVFTWFGMSINTMTLGGLA
                     VAIGELVDDAIVDVENIFRRLRENRHAANPKPALRVVYEASSEVRNSIVFSTILVVLV
                     FVPLFAMGGMEGKLFAPLGVAYIVSIIASLFVSLTVTPILSYWLLAQKRVWPFLAPFM
                     ALGISALVFYWVLPRLAEVVHLPWLHLPGFPLLWTLIGTGPMWLVILLSERYLEADEH
                     EGALLRFLKWITGFAIRLSVRHPWPILGAVAVAVLLSCVVVTQLGLAFLPPFNEGTVQ
                     VNVLLPAGTSLNTSNRIASMVDAKILEIDDVLAIGRRTGRAELDEHAEGVNVSEIIVS
                     IDPHSKRSREEVLEDVKEHVGQVPGVIHAEEQPLQHLMTHMISGIKAQIGIKLFGDDL
                     SVLRATANEIKAEIEGVEGVTGLIVEQQVEIPQLQIKLDRQQLARYGLTSSYVNEFIE
                     TAMNGRTVSEIVQGERKFDLVVRLDDKFRQDVNRIQRLAINLPTGGRIPLEDVATVLP
                     SSGPNTINRENVRRRIVVQCNVSGRPLSEVVADIKQRLEPVQKSLQDAHPGYYIEYGG
                     QFESAEAATRMIGLLGLISLAVMFLALYTLFRSTNLSLQVLSALPMAAIGAVAALVIT
                     GQSLTVASMVGFISLAGIASRNGILLISHYLHLVKYEGEQFTPQMIERAGKERLAPML
                     MTALTAGIALVPLILEAGEPGKEILYPVATVIVGGIISSTLLDFFVHPALFWVFGRKQ
                     AEQHIHDKEGPDELEDGPPAALPTTVVPVREQHVPEGGVIEGGMVAAGS"
     gene            2666..3223
                     /locus_tag="VFV87_07345"
     CDS             2666..3223
                     /locus_tag="VFV87_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103606.1"
                     /translation="MTCRLTWLSLSVAVCAALAFVGCDKTIPPNVSPAESEHHEGDGH
                     EHGEMEHGEEGHSHPTEGPHHGHLIELGEEEYHAELAHDDATKTVTVYLLDKEAKAPV
                     AIPDPEISLNLVVDGQPMQFKLKAAPQEGDPAGQASMFSITDETVLEAHDAPKTTGNL
                     NVTISGKEYSAKIEHAAHGEHEHKK"
     gene            3220..3504
                     /locus_tag="VFV87_07350"
     CDS             3220..3504
                     /locus_tag="VFV87_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103607.1"
                     /translation="MNCRAMIDVWTVLRVLAATIIVVAVADVSQGYPRVGAVVLTLPV
                     VSILAFLAAWFQHRDLPAVSKLARETLVLVPLGLPCFLPLAFAERLGLGF"
     gene            3915..>4860
                     /locus_tag="VFV87_07355"
     CDS             3915..>4860
                     /locus_tag="VFV87_07355"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5103608.1"
                     /translation="MTDEQLAELERSGQAPSRLTIYAPQGGTVVEKLAVEGNYVKAGD
                     PIYRIAELSTVWLMLKLFPEDATRIRFGQRVEATVQSLPGEKLTGRVAFIDPTVNPET
                     RTVGIRVELVNDGRLRPGDYAEASITLPIGAQGSVFDADLAGKWISPMHPQVIRDQPG
                     QCPICGMDLVPTANYGFADEPVPQPVSLHVPRPAVLLAGGDSVVYVETKPGVFEIRPV
                     TLGPILRDKIVILNGLKPGETVATAGNFLIDSQMQLAGKPSLIDPSRAIAKSKERKGP
                     LVFESVTVAPISGEAGQELESLYAAYFQVQQALASDKKP"
BASE COUNT          953 a         1372 c         1486 g         1049 t
ORIGIN      
        1 gttcttgttt ctgctcaact tccgcacgac atttatcacg ctcaccgcga ttcctctctc
       61 gatcgttgta acaggtctgg tctttacgtg gtttggcatg tcgatcaaca cgatgacgct
      121 cggaggcctg gccgtggcaa ttggcgaatt ggtggacgac gccatcgtgg atgtcgagaa
      181 catcttccgg cggcttcgcg agaaccgcca tgctgccaat cctaagccgg cgctgcgcgt
      241 cgtgtatgaa gccagcagcg aggtgcgcaa ctccatcgtg ttcagcacga ttcttgtcgt
      301 gcttgtcttc gtgccgctat ttgcgatggg cggcatggag ggcaagctgt tcgcgccatt
      361 gggagtggcc tacatcgtct cgattattgc ctcgctgttc gtttcgctca cggtcacacc
      421 gatcctgtcg tattggcttc tggctcaaaa gcgcgtctgg ccattcctgg cgcccttcat
      481 ggcactgggc atttcggctt tggtgtttta ctgggtgttg ccccggttgg cggaagtggt
      541 gcatttgccg tggctgcatt tgccgggttt cccgctgctc tggacgctga ttgggacggg
      601 cccaatgtgg ctggtcatcc tgctctcgga gcgctacctg gaggccgacg agcacgaggg
      661 tgcgttactc cgcttcttga agtggatcac cggttttgca attcgcctaa gtgtgcggca
      721 tccttggcca attcttggtg ctgtggcggt ggcggttctc ctaagctgcg tcgtggtcac
      781 tcaattgggc ctcgcctttt tgcctccgtt taacgaaggg accgtgcagg tcaacgtgct
      841 gcttccggct ggcacgtcct tgaatacctc caatcgaatc gcgtcgatgg tggatgcgaa
      901 gattctggaa atagacgacg ttttggcgat tggtcggcga accggccgcg ccgaactcga
      961 cgaacatgcc gaaggcgtta atgtctccga aatcatcgtc agcatcgatc cccacagtaa
     1021 acgcagccgc gaggaagtac tcgaggatgt caaggagcat gtagggcagg ttcccggcgt
     1081 gattcatgcc gaggagcagc cgctgcagca cctgatgacc cacatgatct cgggcatcaa
     1141 ggcgcaaatt ggcatcaagc tcttcggcga tgatttgtcg gtgttgcgcg caacagcgaa
     1201 tgagatcaag gccgagattg agggcgttga aggggttacc ggtctgatcg tcgaacaaca
     1261 ggtagagatc ccgcagcttc agatcaagct cgatcgccaa caactcgcga ggtacggtct
     1321 gacgtcgagc tatgtgaacg aattcatcga gacggctatg aatggccgta ccgtgtccga
     1381 gatcgttcag ggggaacgga agttcgacct agtcgtgcgt ctcgacgaca agtttcggca
     1441 agacgtgaac cgcattcagc gcctggccat caacttgccc acgggcggcc gcattcccct
     1501 ggaggatgtg gcgaccgtgc tgccgagcag cggcccgaac acgattaacc gcgagaacgt
     1561 gcggcgccgt atcgttgtgc agtgcaacgt ctctggacgt ccgctgagcg aagtcgtggc
     1621 cgacatcaag caacgcctgg aaccggtgca aaagtcgctg caggacgccc accctggcta
     1681 ctacatcgaa tacggcggtc aattcgagag tgccgaagct gccacgcgca tgattggact
     1741 cttaggcttg atttcgctgg cggtcatgtt cctggcgctt tacacgctgt tccgttcgac
     1801 gaacctttcg ctgcaagtcc tctcggcgct gccgatggcg gcgattggcg ccgtcgccgc
     1861 cttggtgatc acaggccagt cgcttactgt cgcgagcatg gtcgggttca tttcgctcgc
     1921 cggcatcgct tcgcgcaatg gcattctgtt gatctcgcac tatctgcacc tggtcaagta
     1981 cgaaggcgag caattcacgc cgcaaatgat tgagcgggcc ggcaaggaac gcttggcgcc
     2041 catgttgatg accgctttga cggccggcat cgcattggtg ccgctcatct tggaggctgg
     2101 cgagccgggt aaagagattt tgtatccggt cgccacggtg atcgtcggcg gaatcatcag
     2161 ttcgacacta ctcgactttt ttgtgcatcc cgctctgttt tgggtctttg gacgcaaaca
     2221 agcggaacag catattcacg ataaggaagg ccccgacgag ttagaggatg gcccgcctgc
     2281 ggccctgccg actaccgtcg tgcccgtgag agaacaacac gttccagagg gaggtgtgat
     2341 tgaaggcggc atggtcgccg ccggaagtta gtcgttacca catttgtgtc tcagcacatc
     2401 gcaaggcgtt tacaatgccg ggcccaagct tcagcgttac acattgaagg tgtgcggcac
     2461 agtcactcca tcgtcgtcca acaggcctgc gatgcaggag ctgtgccact tactcggcgt
     2521 ttgaggcggt caatgagagt tccctgcgcc cgttaaacat cgtcatggaa gacagcacgt
     2581 tcagcagtcg gtccgtggag tgacggccca cggctccttg taaaaattcc cgtcaagaac
     2641 ccccatgata tttgggagaa atcgaatgac ttgccgtctg acctggttgt cgctatcggt
     2701 agccgtctgc gctgcgctgg cctttgtcgg ctgcgacaag acgataccgc cgaacgtatc
     2761 gcccgcggaa tccgaacacc acgagggcga tggccacgaa catggtgaaa tggagcacgg
     2821 ggaggaaggg cattcacatc ctaccgaagg accacatcat gggcacttga tcgaacttgg
     2881 cgaggaagaa tatcacgccg aactcgccca tgacgatgcc acgaagaccg taaccgtcta
     2941 tctcttggac aaagaggcta aggcgcccgt ggcaattccc gatcctgaaa tctcgctcaa
     3001 tttggttgtc gatggccagc caatgcagtt caaactgaag gcagccccgc aagaaggaga
     3061 tcctgcgggg caagcctcca tgttttcgat caccgatgag acggtcttag aagcccatga
     3121 tgccccaaag accaccggca atctcaatgt gacgattagt ggaaaggaat attccgccaa
     3181 gatcgaacat gcagcgcacg gagagcacga gcacaagaaa tgaactgtcg cgccatgatt
     3241 gatgtctgga cagtcctgcg tgttttggcc gcaacgatca tagtggtcgc cgtggcggac
     3301 gtatcccagg gctatccgcg cgtgggagct gtcgtcctga ctttgccggt cgtaagcatc
     3361 ctggccttcc tggccgcctg gtttcagcac cgtgacctgc cggctgttag caagctcgcg
     3421 cgggaaacgc tagtcctggt gccactcgga ttgccgtgtt tcctgccgct cgcctttgcc
     3481 gagcggcttg gactgggatt ttaaggcggc gttgtgagcg gcttggttct ggcaagtgga
     3541 acaattctgg cgtggatcgc ctggggtcct aaattgtaat tcggtctcgc ggtgatcgca
     3601 gtcatcagca cgtgtccgag cgaattcgtt ccatcgcttt ctaactcgcc atgaagacac
     3661 cgccagcgcc agcattcagg gcatgggttg tgccgaggag gtggtaatcc tgaagcgcga
     3721 ggtgggacgg gtcattggcg gcgggaatcg actctcgttc gacattctga aagggcggat
     3781 ggtggtctct gacgatggac cgccggtttc cgtggaagtc gtgattgaag ctgttttgag
     3841 gacggggatg cgggcggaga tttggtgcag aagcactcgc ggccaatacg cgtcagcgct
     3901 tgcgtgagtt tggcatgacc gatgagcaac ttgccgagct ggaacggtcg ggccaggcac
     3961 cgtcgcgact gaccatttac gccccccaag gcggcacggt cgtcgaaaaa cttgccgtcg
     4021 aaggcaacta cgtcaaagcg ggcgacccga tctatcgcat tgcagagctt tccaccgtgt
     4081 ggctgatgct gaagctgttt cccgaggatg cgacgcggat tcgttttggc cagcgcgtgg
     4141 aggccacggt ccagtcgctg ccgggcgaga agctgacggg ccgcgtggcg ttcattgatc
     4201 cgacagtcaa tcccgaaacg cgcacggtcg gtatccgcgt cgagctggtc aacgacggcc
     4261 gcctgcggcc gggcgactat gccgaagcgt cgatcacgct gccgatcggt gcgcaaggca
     4321 gcgtattcga tgcggactta gccggtaaat ggatcagccc gatgcacccg caagtaattc
     4381 gggaccagcc gggccagtgc ccgatctgcg gcatggatct ggtaccgacc gccaattatg
     4441 gatttgccga cgagcctgtg ccgcagccgg tgtccttgca cgtgccgcgg cccgctgtgc
     4501 tgttggccgg cggcgacagc gtcgtctatg tggaaacgaa gccgggcgtg ttcgaaattc
     4561 gccccgtgac gctcggtccg atccttcgcg acaagattgt catcctcaac ggcctgaagc
     4621 cgggcgagac ggttgccacg gccggcaact tcctcattga ctcgcagatg cagctcgccg
     4681 gcaagccgag cctgatcgac ccttcgcggg cgattgcaaa aagcaaggag cggaaaggcc
     4741 cgctcgtgtt tgaaagcgtc acggtcgcgc ccatcagcgg cgaagccggc caggagctcg
     4801 aaagccttta tgccgcctat ttccaggtgc agcaggcgct cgcttccgac aaaaagccgc
//
LOCUS       DASUGB010000382         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_459821, whole genome shotgun sequence.
ACCESSION   DASUGB010000382 DASUGB010000000
VERSION     DASUGB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459821"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..672)
                     /locus_tag="VFV87_07360"
     CDS             complement(154..672)
                     /locus_tag="VFV87_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5103609.1"
                     /translation="MNAIAREPAEILLVDDDPLALTAAAAALDMAGYIVHQAQDRRAA
                     LKAARTLALDLVICDVNLAGESGLELWRELRKLAGMEDVPVMFVSATQLPDIVRRSHE
                     AGGAYYLRKPLDPEVLVELVGKALWLPHLVQSRVAMHQPAAQAVPAPAARPSAATLSM
                     TAAITGIRLPLA"
     gene            complement(877..1545)
                     /locus_tag="VFV87_07365"
     CDS             complement(877..1545)
                     /locus_tag="VFV87_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5103610.1"
                     /translation="MPRHIFLFVAVFALAPIARADEPAKPAESKWEKTIAAFEEQDKL
                     SPPTPGGVVFYGSSSIRLWDLKKSFPDINAINRGFGGSQMSDAAQFVKRVVTPYKPRA
                     IVLYEGDNDLNANKSPEQVAADFEALRKIVRGDLPGAKLIVIGCKPSPSRWKLIEKQR
                     ELNRLLAARCAVDEHAAFVDIEQPMLGTDGQPREELFRADKLHLSDAGYELWNSLVQP
                     LIAP"
     gene            complement(1577..1768)
                     /locus_tag="VFV87_07370"
     CDS             complement(1577..1768)
                     /locus_tag="VFV87_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103611.1"
                     /translation="MAKRSKTQAVKPAKGPRADNPATAQSAPTIIAQPPEKSISLLGI
                     SILLFALWFIFLLVTALVG"
     gene            complement(1817..>3035)
                     /locus_tag="VFV87_07375"
     CDS             complement(1817..>3035)
                     /locus_tag="VFV87_07375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX5103612.1"
                     /translation="LGVGREAYWQALEASQSGIDWFPATRGTELPFPFAGMLRDFDPK
                     QYIQPRKSIKVMCREIQTAYACASLAVQDAGLPKEAAEPDRMGIVLGSELFYGELDEL
                     GDVYRHCHDNGQFRTERWADYAFKNLFPLWMLKYLPNMAASHISIVHDARGPNNSIVQ
                     GGASSLLAMMEAAAAIQRGSADIMLAGGSGSNAELTGIAFRGWEQHSQWPGQPEESSR
                     PFDAQRCGIVVGEGAATFVLEERGHAERRGAQILARIAGFSSRYEGRGGWNVPRTGAG
                     IRSSIAGALNAAGMSPGDIGHVNAHGDSSIHGDRAEAQAICETLGDVPVTAIKSYFGD
                     LGAGSGAVELAASILGLVHGRIPPTRNYDQPDPECPVNVIRAEPLPVDKPVILKLNQS
                     LTGQAVAMVVTGV"
BASE COUNT          542 a          997 c          951 g          545 t
ORIGIN      
        1 atgcatcatg gcgatcgcgt tggccagcgg aaacggtcct cccttcccgg atccgccttt
       61 gtggccgatc ggcgcaggca ttgtcctggg tatcgtcgcg ggagcgtacg tcctgtggct
      121 tacacgaaat gcggccaagg acatttgaag cggtcaagcc agcggcaagc ggatgccggt
      181 gatcgcggcg gtcatcgaca gcgtcgctgc cgagggccgg gcggccggtg cgggcaccgc
      241 ctgagcagcc ggctggtgca tcgccacgcg cgactggacc aggtgcggca gccagagggc
      301 cttgcccacc agttcgacca gcacttccgg gtcgagcggc ttgcggaggt agtacgcccc
      361 gcccgcctcg tgcgagcggc ggacgatgtc cgggagctgc gtggcgctga cgaacatcac
      421 cggcacgtcc tccatgccgg ccagcttccg caattcgcgc cacagctcca ggccgctctc
      481 gccggcgagg ttcacgtcgc agatcaccag gtccagagcc agcgtgcgag cggccttcag
      541 cgccgcccgg cgatcctggg cctggtggac gatatagccg gccatatcga gcgccgcagc
      601 cgccgcggtc aaggccagcg gatcgtcatc gacaagcaga atctcagccg gttcccgagc
      661 aatagcgttc atcggcagcg acactcccgg agtatgggaa cgttcgccgc tccatcggca
      721 ggggcgcgtg gcgtgcgcac tctcagaccg cctgtctgcg tacaggggtt atcgacgcgg
      781 aaagcagcga gaggtcaatc cagtttgcct ctgccgcaca gccgccatat caggaagaag
      841 cggaacggcc gatacttttc tacagggcaa tcagcgttac ggggcgatca gcggctgcac
      901 aagcgaattc cacagctcgt agccggcgtc gctcaggtgc agcttgtcgg cacggaatag
      961 ttcctcgcgc ggctggccat ccgtgccgag catcggctgt tcgatatcga cgaaagccgc
     1021 gtgctcatct actgcacagc gggcagccag caggcgattc agctcgcgct gcttttcgat
     1081 cagcttccac cgcgaggggc tgggcttgca gccgatgacg atcagcttgg ccccgggcaa
     1141 atcgccgcgg acaatcttcc gcaaggcctc gaaatcggca gcgacttgct cgggagactt
     1201 gttcgcgttc aggtcgttat cgccttcgta cagaacgatt gcccgcggct tatagggcgt
     1261 aacgacccgc ttgacgaatt gtgcggcgtc ggacatctgc gagccgccaa agccgcgatt
     1321 aatggcgttg atgtcgggaa acgacttctt cagatcccac aagcgaatac tggagctgcc
     1381 gtaaaagacg acgccaccgg gcgttggcgg cgacagcttg tcctgctcct cgaacgcggc
     1441 gattgttttt tcccacttgc tttccgcagg cttcgccggt tcatcggcac gggcgatcgg
     1501 cgcgagggcg aaaacggcaa cgaacagaaa aatgtggcgg ggcatgcggg tggctccaag
     1561 ttggggaacc tgcgcattat ccaacgagcg ccgtcacaag caagaagata aaccacaggg
     1621 cgaacaagag gatgctgatc cccagcagcg aaatgctttt ttccggcggc tgggcgatga
     1681 tcgtcggcgc ggattgggca gtggctgggt tgtctgcgcg tggccctttc gccggtttca
     1741 ccgcctgagt tttgcttcgc ttcgccatag cacgtccctt cgctggcagt acatccttgc
     1801 aaaatgcgcg ccgctctcaa acgccagtta cgaccatcgc gaccgcctgg ccggtgagcg
     1861 attgattgag cttcaatatc accggcttgt ccaccggcaa tggctcggcg cgaatgacat
     1921 ttactggaca ttcgggatcg ggctggtcgt agtttcgcgt cggcggaatc cggccatgca
     1981 ccaggcccaa aatgctcgcg gccagctcca ccgccccgct gccggcgccc agatcgccga
     2041 aatagctctt gatggccgtg accggcacgt cgcccagcgt ctcgcaaatc gcctgagcct
     2101 cggcccgatc accatgaatg ctgctgtcgc cgtgggcgtt gacgtggcca atgtcgccgg
     2161 gagacatccc cgcggcattc agcgctcccg cgatcgaact gcggattccc gctcccgtcc
     2221 gcggcacgtt ccagccgccg cggccttcat accggctgga gaatcctgcg atgcgggcga
     2281 gaatctgcgc cccacggcgc tcggcatggc cacgctcttc gaggacgaag gtcgccgccc
     2341 cttcgccgac gacgatccca caccgctggg catcgaacgg ccgcgacgat tcttccggct
     2401 ggcccggcca ctgcgaatgc tgttcccagc cccggaaagc gatccccgtc agctcggcat
     2461 tcgaaccgct gccaccggca agcatgatgt cggcactgcc gcgctgaatc gccgccgccg
     2521 cttccatcat cgccagcagg ctggatgcgc cgccttgcac gatcgaattg ttcggcccgc
     2581 gggcatcgtg gacgatgctg atgtgggagg ccgccatgtt cggcaggtac ttcagcatcc
     2641 acagtggaaa gaggttcttg aacgcgtaat cggcccaccg ctcggtgcgg aactgcccgt
     2701 tgtcgtggca gtgacgatag acatcaccca gttcgtccag ctcgccgtaa aacagctcgc
     2761 ttcccagcac gattcccatc cggtccggct cggcggcttc cttcggcagg cccgcatcct
     2821 gcaccgccag cgacgcgcag gcgtacgcgg tctggatctc gcggcacatc accttgatcg
     2881 acttgcgggg ctgaatgtac tgcttgggat cgaagtcgcg gagcatcccc gcgaagggaa
     2941 agggcaattc ggtgccgcgg gtcgcgggga accagtcgat gccgctctgg ctggcttcca
     3001 aggcctgcca ataggcctcg cggcccaccc ccaag
//
LOCUS       DASUGB010000383         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_42968,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000383 DASUGB010000000
VERSION     DASUGB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42968"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /gene="asnB"
                     /locus_tag="VFV87_07380"
     CDS             complement(<1..1783)
                     /gene="asnB"
                     /locus_tag="VFV87_07380"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5103613.1"
                     /translation="MCGITGAVWNDPRLGLDEPQLVRMTDAIRHRGPDDEGTYLSEYR
                     LRPPYEAQPGIALGFRRLSIIDLAGGHQPMANEDESVWIVFNGEIYNYRDLRRRLEGA
                     GHKFKTESDTECIVHLYEDEGPDCFTHLNGMFAVAIWDARNRRLVLGRDRLGKKPLVY
                     RNEGSRLLFASELKSLLTVPGLPREVDPAAVDEYLTYQYVPHPNCILKGYRKLPPGHY
                     AQWQEGKLEVRPYWQPDFSHEISISEKDAAERLRELLESAVTMRMRSDVPLGAFLSGG
                     VDSSLIVALMQKNSEQPVKTFSIGFPVAEYDETKYARQVAKHLKTEHHELQVTPDAVG
                     ILPKLARHYDEPFADSSAIPTWYVSQMTREHVTVSLSGDGGDELFAGYPRYRAVAMAG
                     MFDYLGPLRSLLAARFWQRLPASSRQKSRLRQFKRFSEALSLSPARRYLDWISIFNEG
                     RRAALYSDEFLAELPDSDPFAFLNSAWKKMQGRDAVTTASLGDLITYLPCDLMTKVDI
                     ASMANSLECRAPFLDYRVAELAAAMPLAYKLHRGRPKRILREAFPELLPAEVTARPKM
                     GFGVPLAQWFRGELKDYARDVLLDSRAV"
     gene            1910..>2434
                     /locus_tag="VFV87_07385"
     CDS             1910..>2434
                     /locus_tag="VFV87_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103614.1"
                     /translation="MKSTLNFRPNFTALLLLSTFAAPSWLAAQEEAPGGPDRGAMIRK
                     IDERIAERLATAKIAPAPIADDAEFLRRVYLDLTGVVPRVSEVREFLADKRPDKRAKL
                     IDKLLDSPAHATHLANTWRNIMLPGGLNIEQIQNVVGVQNWLRTRFVDNLRYDNMVSE
                     LLVATEGDDVGPALY"
BASE COUNT          465 a          756 c          771 g          442 t
ORIGIN      
        1 ccacggcccg cgaatcgagc agcacgtccc gggcgtaatc cttcaattcg ccgcgaaacc
       61 actgggccag cggcacgccg aagcccattt tgggtcgagc cgtgacctcg gccgggagca
      121 gctcgggaaa ggcttcccgc aagatccgct tcgggcggcc gcggtggagc ttataggcca
      181 gcggcatggc ggccgccagc tcggccacgc gatagtcgag aaacggcgcc cggcactcca
      241 ggctgttggc catcgacgcg atgtcgacct tggtcatcag gtcgcacggc agatacgtga
      301 tcagatcgcc aagcgacgcg gtcgtgacgg catcccggcc ttgcatcttc ttccaggcgg
      361 aattcaggaa tgcgaacgga tcggagtccg gcagctccgc gaggaactcg tcgctgtaga
      421 gggccgcccg ccggccttcg ttgaagatgg aaatccagtc gagataccgc cgcgccggcg
      481 aaagcgacag cgcttcgctg aaccgcttga actgccgcag gcgcgacttc tgccgcgacg
      541 aggccggcag acgctgccag aaccgggcgg cgagcagcga acggagcgga ccgagatagt
      601 cgaacattcc cgccatcgcc acggcccgat accgcggata gccggcaaac aattcgtcgc
      661 cgccatcgcc gctcagcgag acggtgacgt gctcgcgggt catctgcgag acgtaccagg
      721 tgggaatggc cgagctatcc gcgaacggct cgtcatagtg ccgggccagc ttcggcagaa
      781 tgccgaccgc atcgggagtg acctgcagct cgtggtgctc agtcttgaga tgtttggcaa
      841 cctggcgggc gtacttcgtt tcgtcgtatt ctgccaccgg aaagccgatg ctaaacgttt
      901 tcaccggctg ctcggagttc ttctgcatca acgccacgat gagggacgaa tcgacgccgc
      961 cggaaagaaa cgcgccgagc ggcacatcgc tccgcattcg catcgtgacg gcggattcga
     1021 ggagctcccg caggcgttcg gcggcgtctt tttcgctgat gctgatttcg tgcgaaaaat
     1081 cgggctgcca gtaagggcgg acttcgagct tcccctcctg ccactgcgca tagtggccgg
     1141 ggggaagctt gcgatagccc ttgagaatgc agttgggatg cgggacgtac tgatacgtca
     1201 gatattcatc gacggcagcg gggtccactt ctcgcggcag gccagggacc gtcagcaggc
     1261 tcttgagctc gctggcgaac agcagccggc tcccctcgtt gcgatagacc agcggctttt
     1321 tgccaagccg gtcgcggccg aggaccagcc ggcggttgcg ggcgtcccaa atggcgacgg
     1381 cgaacatgcc gttgagatgc gtgaagcaat ccgggccttc gtcttcgtag agatggacga
     1441 tgcactcggt gtcgctctcc gttttgaact tgtgccccgc cccttccagc cgccgccgca
     1501 ggtcgcggta gttgtaaatc tccccgttga acacgatcca aaccgactca tcctcgttag
     1561 ccatcggctg atggccgcca gccaggtcga taatcgacaa tcgccgaaag ccgagggcga
     1621 ttccgggctg ggcctcgtag gggggacgga gacggtactc gctgagatag gtcccctcat
     1681 cgtccggccc ccgatgccgg atcgcatctg tcatccgcac cagttgcggc tcatccagcc
     1741 ccagccgcgg atcattccaa acagcgccgg tgatgccgca catgggttag gtatttgccg
     1801 cggaaaatcg aggaaaatgg agcgacgtag tatagctgac ggaatgaaaa ggttgtgaat
     1861 aaccgtgcgt ctcgttagaa taaaccggtc tttctttgtg gaggtctcca tgaagtccac
     1921 gctgaacttt cgcccgaact ttaccgccct tctgctgctt tctacgttcg ccgccccttc
     1981 ttggttggca gctcaggagg aagctccggg cggacccgac cggggggcga tgatccgcaa
     2041 gatcgacgag cggattgcgg agcggctcgc gacggcgaag atcgcgcccg ccccgattgc
     2101 ggacgatgcc gaattcctcc gccgggtcta tctggatttg accggcgtgg tgccacgcgt
     2161 ctccgaggtt cgcgagttcc tggcggacaa gcggccggac aaacgagcaa agctaatcga
     2221 taagctcctc gattcgccgg cccatgccac gcacctggcg aacacctggc ggaacatcat
     2281 gctgccaggc gggctgaaca tcgagcagat tcaaaatgtc gttggcgtgc agaactggct
     2341 gcggacccgc ttcgtggaca atctgcggta cgacaacatg gtcagcgagc tgctcgtcgc
     2401 caccgagggg gacgacgtcg gcccggctct ctat
//
LOCUS       DASUGB010000384         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1293642, whole genome shotgun sequence.
ACCESSION   DASUGB010000384 DASUGB010000000
VERSION     DASUGB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293642"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFV87_07390"
     CDS             complement(<1..1453)
                     /locus_tag="VFV87_07390"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5103615.1"
                     /translation="MTATSPAPAKPKFEAPHPGSKAMPRWDTGELIDAPIFTTRNLLQ
                     MIGPGLVMGAAAIGGGEWLAGPAVTAKYGGALLWVAAVSILFQVIYNIEISRYTLYTG
                     EPIFTGKFRLLPHPMFWLLLYLALDWGSVAPYLAVNAAVPLESMILGRLPEAGTNVGD
                     WWFHKGVCTGLYLIIMVPLIFGGKIYDALKIVMGTKLVVVVGFLLLMGLFFARPASWV
                     EIFTGFLKVGTVPVVVEQPPETPPSGDAAAPGGEIKNTENVFITLFTEGRLPKLDWSL
                     IAVIAGLAAIAGNGGLTNTPISNFTRDQGWGMGHHVGAIPSVVGGHGITLSHVGCVFD
                     VNEETLPRWRRWYRHICRDQLVVWMGACIIGCALPSILSVGFLPRGTDVSGYNVAAMT
                     AEATRKQGIEPLSGTLVTATGLDRVIAGPRLGAFFFYCTLFCGFMVLITSQTTTMDGF
                     CRRWVDVIWTASAKMRELDTSKIRYVYFTLLCIY"
     gene            complement(1616..>2499)
                     /locus_tag="VFV87_07395"
     CDS             complement(1616..>2499)
                     /locus_tag="VFV87_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103616.1"
                     /translation="AGRRENWHEFVPHQPALEMLRLLPWSAAVVFVLADPDGWLRGAS
                     SRPEDAGVTNERRLLLALVWLLVPLAAAWLLTETDVARLFHLRYLAVSAPAAILIVAT
                     LVRLPPLPTSRVVLAIGIVAYALWSSEIVQQVRYDGRAIADRREDWRSAVGWLNKRIS
                     DDPAPILVRSGLIEADALRSEHDRRFADYCLLPVVSLYRLGADRADLHPLPYHNAGRL
                     QPDVQRELLRRGGGWLVIRGSERLSASIEQRIIRSLAATDKRPWRVAERRSFGNVQVC
                     RLSLRERASVRGASGDN"
BASE COUNT          471 a          819 c          825 g          384 t
ORIGIN      
        1 cataaatgca gagcagcgtg aaatagacgt agcggatctt gctcgtgtcg agctcccgca
       61 ttttggcgct ggcggtccag atgacatcga cccagcggcg gcagaagccg tccatggtgg
      121 tcgtttggct ggtgatcagc accataaagc cgcaaaacaa cgtgcagtag aagaagaagg
      181 cccccagccg cggcccggcg atcacccggt ccagacccgt ggccgtgacc agcgtgccgc
      241 tgaggggctc gatgccctgt ttcctcgtcg cttccgccgt catcgcggcc acgttgtagc
      301 cgctcacgtc ggtgccccgc ggcaaaaagc cgaccgacag aatgctgggc aacgcgcagc
      361 cgatgatgca ggcccccatc cagacgacga gctgatcgcg gcagatgtgc cggtaccagc
      421 gccgccagcg cggcagcgtt tcctcgttga cgtcgaacac gcagccgacg tgcgagagcg
      481 tgatgccatg cccgccaacg acgctgggaa tggcccccac gtgatgcccc attccccagc
      541 cttggtcgcg cgtgaagttg ctgatcggcg tgttggttaa gccgccgttg ccggcaatgg
      601 cggccaggcc cgcgatcacg gcaatcagcg accaatcgag cttcggcagc cggccttcgg
      661 tgaagagcgt aatgaagacg ttctcggtgt ttttgatttc gccgccaggc gcggccgcgt
      721 cgccggaagg cggcgtttcg ggaggttgct cgacgacaac cggcaccgtt ccgactttga
      781 gaaatcccgt gaaaatctcg acccagctcg ccggccgtgc aaagaacaaa cccatgagca
      841 gcagaaaacc gaccacgacc accagctttg tccccatcac gatcttcagc gcgtcgtaga
      901 tctttccgcc gaagatcagc gggaccatga tgatcaagta gagccccgtg cagactccct
      961 tgtggaacca ccaatcgccg acatttgtgc ccgcctcggg aaggcggccc aggatcatcg
     1021 actccagcgg cacggcggcg ttcaccgcca ggtagggcgc caccgagccc cagtcgagcg
     1081 ccaggtacaa aagcagccag aacatcgggt gcggcagaag gcggaatttc ccggtgaaga
     1141 tcggctcgcc ggtatagagc gtgtagcggc tgatctcgat gttgtagatg acctggaaga
     1201 ggatgctgac ggcggccacc cagaggagcg ccccgccgta tttggccgtg acggctggcc
     1261 ccgcgagcca ttcgccgccg ccgatggccg cggcccccat gacgaggccc gggccgatca
     1321 tctgcagcag attccgcgtg gtgaaaatgg gggcgtcgat cagctcgccc gtgtcccacc
     1381 gcggcatcgc cttcgatccg gggtgcggcg cttcgaattt cggtttcgcg ggggcgggag
     1441 aagtggctgt catcgggccg tctggggaga ggttgcggcg gagtggccta tgatgggccg
     1501 gcgcagcggg caggtcaacc atcaggagga aaaggggtcg ggagtcaatt gcggtaacgg
     1561 cactccattt ggaaaacgtc cccgcaattg actcccgacc cccttggcgc cgacctcaat
     1621 tgtcgccgct ggctccgcga acggacgccc tttcgcggag cgaaaggcga cagacttgca
     1681 cgttcccgaa cgagcgccgc tcggcgacgc gccacgggcg cttgtctgtg gctgcgagcg
     1741 agcggatgat ccgctgctcg atgctcgccg acaatcgctc gctgccgcgg atgacgagcc
     1801 acccaccgcc acggcgcaac agctcgcgct gcacgtccgg ctgcagtcgg cccgcgttgt
     1861 ggtacggcag gggatggagg tccgcgcgat cggcgcccag gcgatagagt gaaacgaccg
     1921 gcagcaggca ataatcggcg aaacgccggt cgtgctcgct gcggagggcg tccgcttcga
     1981 tcaggccgct gcggacgaga atcggcgctg gatcgtcgct gatccgcttg ttgagccagc
     2041 cgacagccga cctccaatcc tcgcggcgat cggcgatcgc gcggccgtca tagcgaacct
     2101 gttgcacgat ctcgcttgac cagagggcgt aggcgacgat gccgatcgcc agaacgacgc
     2161 gggaagtcgg cagaggcggc aggcgcacga gcgtcgcgac gatcaagatc gccgccggag
     2221 cgctgaccgc cagataccgc aagtgaaaca gacgcgcgac atccgtttcc gtcagcagcc
     2281 aggcggcggc cagcggcacc aacagccaca ccagcgcgag cagcagccgg cgctcgttcg
     2341 tcactcccgc gtcttctggc ctcgagctgg cgccgcgcag ccagccgtcc ggatcggcca
     2401 ggacgaacac gaccgcggct gaccagggga gcagccggag catctccagg gccggctggt
     2461 gcgggacgaa ctcgtgccag ttctcgcgcc gcccggcga
//
LOCUS       DASUGB010000385         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_460277, whole genome shotgun sequence.
ACCESSION   DASUGB010000385 DASUGB010000000
VERSION     DASUGB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460277"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFV87_07400"
     CDS             complement(<1..693)
                     /locus_tag="VFV87_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5103617.1"
                     /translation="MPLPLKPLPIVERWSCHQCGVCCRGSIVPLSPADLARLKEQGWA
                     EHPDFRGTAITTRESWLGGEHRLAHREDGSCVFLLPDGLCRIHKELGFDAKPLICRTF
                     PLQVVPRDNAAIVTIRRACPSAAADRGQPVAEQLDQARPVIRESGLADAAVRPPAVKP
                     GERRDWRTTRRVLDALQRLLCDERFPIVRRLVHSLTFCRLIEQAQTRRLADDQLAELC
                     TVLEQAAPDESSD"
     gene            782..1933
                     /locus_tag="VFV87_07405"
     CDS             782..1933
                     /locus_tag="VFV87_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103618.1"
                     /translation="MGLAIALWQMLISKRLAILGAGGLWLTFAAAASAAPPWATLIPF
                     KRIEADAEKSYELDESHGPWLIIAASFAGPTAQQQAHDLVIELRQRYKLEAYTFEKVY
                     DFTNTTEGLGVDEFGSPKRMKYMHAAKFQEIAVMVGNFASIEDPELEKTLDKIKYAKP
                     DVLQRDVNNKTQRMAVVRALYRFVDSNPATRSKGPMGSAFVTRNPLLPEEYFAAKGLD
                     PFLVDLNRDLPNSLLECPGRYTVRVASFRGVDTMKPAEFERLTSKPRDMAKIDQAAQK
                     AYDLCAALRQQGYEAYQFHDRTESIVTVGSFDSVGTPRQDGKTEINPAVHEIMKRLGP
                     SSARLPGQAAMGLAPKVVAGIALDPQPLPVEVPRESIAAAYNQTKTMLR"
     gene            2072..>2340
                     /locus_tag="VFV87_07410"
     CDS             2072..>2340
                     /locus_tag="VFV87_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103619.1"
                     /translation="MPLRVRCPTGHKLIVPDDRAGRSLRCPRCGEVTQVPGEARQGSA
                     VRDQGSGDRSEVSGDQETEGTRRRDSSSVLDWPNSIVAPTALASPP"
BASE COUNT          422 a          681 c          861 g          376 t
ORIGIN      
        1 gtcgctgctt tcgtccggcg cggcctgctc caggaccgtg cagagctcgg cgagctggtc
       61 gtcggccaga cggcgggtct gggcttgctc gatcaagcgg cagaacgtga gcgagtgaac
      121 gaggcggcgg acgatcggaa accgctcgtc gcaaagcagg cgctggagtg catcgaggac
      181 gcggcgtgtc gtgcgccagt cgcggcgctc gcccggcttc acagcgggcg gacggaccgc
      241 ggcgtcggcc aggccgcttt cgcgaatgac cgggcgggcc tgatcgagtt gctcggcaac
      301 cggctggccg cggtcggccg ccgcggaggg gcaggcccgg cggatcgtga cgatggcggc
      361 gttgtcccgc gggacgacct gcagcggaaa cgtgcggcag atgagtggct tcgcatcgaa
      421 gcccagctcc ttgtggatgc ggcacaggcc atcgggcaac agaaagacac agcttccgtc
      481 ctcgcgatga gccaagcggt gctcgccgcc gagccagctt tcgcgagtgg tgatggcggt
      541 gccgcggaaa tcgggatgtt cggcccagcc ttgctccttc aaccgggcca gatcggcggg
      601 cgacagcggc acgatgctgc cacgacagca gacgccgcat tgatggcagc tccagcgttc
      661 gacgatcggc agcggcttga gcggaagagg catacgtccg ttctcccgcg aatcgctctg
      721 ggcagcaagc gggcgattgg gtatggtccg gccgcttttc tctggtcttg ggagtgggaa
      781 tatgggactg gccatcgcac tttggcaaat gctgatcagc aaacgacttg cgattctcgg
      841 ggcaggcggg ctgtggctca cttttgcggc cgcggccagc gccgcgcccc catgggccac
      901 gctgattccg ttcaagcgca tcgaagccga tgcggagaag agctacgagc tggacgaatc
      961 gcacgggccg tggttgatca tcgcggcttc gttcgcgggg ccaaccgccc agcagcaggc
     1021 gcacgacctg gtgatcgaac tgcggcagcg ctacaagctg gaagcctaca cgtttgagaa
     1081 agtgtatgac ttcacgaata cgacggaggg tctgggtgtc gatgagttcg gaagtccgaa
     1141 gcggatgaag tacatgcacg cggcgaagtt ccaggagatc gccgtgatgg tcggcaactt
     1201 tgcgtcgatt gaagacccgg agctggagaa gacgttggac aagatcaagt acgccaagcc
     1261 cgacgtgctc cagcgcgacg tcaacaacaa gacgcagcga atggctgtcg tgcgagcgct
     1321 gtatcggttc gttgattcca atccggcgac gaggagcaaa gggccgatgg gcagcgcgtt
     1381 cgtgactcgg aacccgctct tgcctgagga gtatttcgcg gccaaggggc tcgacccgtt
     1441 tctggtcgat ctgaaccgcg atttgccgaa cagcctgctc gagtgcccgg ggcgatacac
     1501 ggttcgggtc gccagcttcc gcggcgtcga tacgatgaag ccggcggagt tcgagcgcct
     1561 gacgagcaag ccccgcgaca tggcgaagat cgaccaggcg gcgcagaagg cgtacgacct
     1621 gtgcgcggcg ctgcggcagc agggttacga ggcgtaccag tttcacgacc ggaccgagag
     1681 cattgtaacg gtcggatcgt tcgattcggt cggcacgccg cggcaggacg ggaagacgga
     1741 gatcaatccg gcggttcacg agatcatgaa acggctgggg ccgagcagcg cccggctgcc
     1801 ggggcaggcg gcgatgggcc tggcgccgaa ggtggtggcg ggaattgccc tcgatccgca
     1861 gcccttgccg gtggaagtgc cgcgggaatc gatcgcggcg gcgtataacc agacgaagac
     1921 gatgctgcga taagctccaa agccgcgacc agcggtcgcg gttgctggtg gggcctgcgg
     1981 tgccagttga ccttctgccg gcagcgggca agactggaat cacgggcgca gcgctgcgcc
     2041 gctactggtt ccagccgctg ggcgtccttt catgccgctg cgcgtccgct gtccgacggg
     2101 gcacaagctg attgtgcccg atgaccgcgc cgggcgatcg ctgcggtgcc cgcggtgcgg
     2161 cgaagtgacg caggtgccgg gcgaagcgag gcaggggtca gcggtcaggg atcaaggttc
     2221 aggggacagg agcgaggttt caggggatca ggagacggaa ggtacgcggc ggcgggatag
     2281 cagcagcgtg ctggattggc cgaattcgat tgtggcgccg acggcgctgg ccagcccgcc
//
LOCUS       DASUGB010000386         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1849036, whole genome shotgun sequence.
ACCESSION   DASUGB010000386 DASUGB010000000
VERSION     DASUGB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849036"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VFV87_07415"
     CDS             <1..1773
                     /locus_tag="VFV87_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103620.1"
                     /translation="EFGWSLPAGEGPFGAAPLASLLGQAGLGWAKLPVWYDAHDNQES
                     ERIAWFAEQLSIQGIELVGVLDQPPKELRQVFREEGRLPVASVFAEPELWEPAVSPVL
                     TRLSLRIRWWQLGEDQDTSFVGMPQAEAKIGEIRRRLEQYGQELRLGVGWRWLHPTPE
                     LRLDDRPPWSFLSYNAEPALTAAELSTYLAPAGPAEVKAASKTLDSSLQRRSHTPRPG
                     WSAQVAPPSAVASWVMLSPLAREEYTLSARVQDLAQRMLSARVHGASAVFVPLPFDAS
                     HGLINADGSPGELFVPWRTTATLVGGAEYLGSIQLPGGSTCHAFGRDGQAVLAIWNEQ
                     PTTERLTLDDGVEQIDVWGQNVLLPIEAQAEPQEREIAVGPLPTFLIGQSEGVARWQA
                     TVDFESLQVASVFNVEQSINLKIRNPFNQGISGEVKLQAPASWGVDPRPTRFKIAVGD
                     ELRLALPVTLLADANSGPQPVRLDFEITADRNYRFSVYRTLQLGLDDVQIELRTKLRN
                     DGALIVEQQLNNLSERPVSFQCLLFPQGRRREIRQVINAQPGQTATTYILPRGEELIG
                     QRLLLRAEEIGGPRVLNYTLTGER"
     gene            1993..>4501
                     /locus_tag="VFV87_07420"
     CDS             1993..>4501
                     /locus_tag="VFV87_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5103621.1"
                     /translation="MIRLFQNALCCVLPLLLAAGLAKADEPKLKLVKGDRIVIVGNTL
                     AERLQQHGHFETLLHSRFPDLELVVRNLGWSADEISLRPRSKDFKDHGHTLADHDPTV
                     ILAFFGFNESFAGPAGLSQFEADLESFLKAPQNIDRYSTARSNWDKTPDTAQELPPLK
                     SLRQIVLVSPIAFNALHDSHHPDGNVQNKNLKLYSEVMARIAAKHKVPFVDLFLSTLP
                     LMEYPRYATPLTFNGIHLNEKGDQAVSRILDVALFGPRPENRANIDLEVLRQAVNEKD
                     RLFWLDYRAVNGFYIYGGRKEPFGVVNFPAEFAKLRKMIANRDQRIWAVAQGKEVPPT
                     IDDGNTGVNPKIETNFKNLISITPPEEAQKTFTLPEGYAINCFASEVDFPDMEDPVAF
                     AFDNKGRLWVCCMPSYPQYLPGKQINDKILILEDSDGDGKADKQTVFADKLYLPAGME
                     FWNGGVIVAQQPNVLFLKDTDGDDKADIRETILHGFDSADSHHAISAFTFDQGGALYF
                     QEGTFHHSQIETPFGPRRCVNAGVFRFEPRTDKLDVFISYSFANPWGHIFDPWGQDFV
                     ADASPGFNYFAAAFSGDVDYPDKHGSLKQILVKQWRPTCGCELVSSRNFPDEAQGNYL
                     LNNCIGFQGTLQYKLKDDGAGFAADPVDPLLQSSDPSFRPVDIEFAPDGSLFICDWFN
                     PLIGHMQHSIRDPNRDHSHGRIWRIQYTKKPLVRPVKIDGEPIPALLKLLETEPEERT
                     RARVRAELWNRPTKDVLAEATKWLAAQDKAGKDFEHHKLEALWLHQAHDSVNEPLLKQ
                     LLRSPDYRARAAATRVLCYWRDRVTQPLELLRAQVNDD"
BASE COUNT          850 a         1433 c         1426 g          792 t
ORIGIN      
        1 gagttcggct ggtcgttgcc ggcaggcgaa ggtccgttcg gggcggctcc gctggcgtcg
       61 ctcctggggc aggccgggct gggctgggcc aagctgccag tctggtacga cgctcacgac
      121 aaccaggaga gcgaacgcat cgcctggttc gccgagcaat tgagcatcca gggcatcgag
      181 ctggtcgggg ttctcgatca accgccgaag gaattgcggc aagtgttccg cgaggaaggc
      241 cgattgccgg tggcctcggt gtttgccgag cccgagctgt gggagccggc ggtgagcccg
      301 gtgctgacgc ggctgtcgct gcggattcgc tggtggcagc tcggcgagga ccaggatacg
      361 agcttcgtcg gcatgcctca ggcggaagcg aaaatcggcg aaatcaggcg gcgtttggaa
      421 cagtacgggc aggagctgcg gctgggcgtg ggctggcgct ggctgcatcc gacgcccgaa
      481 ctgcgactag atgatcgtcc gccgtggtca ttcctttcgt acaatgccga gccggccctg
      541 acggccgcgg agctttcgac gtatttggcg cccgcagggc ctgcggaggt caaagccgcc
      601 agcaagacgc tggattcttc gctccagcgc cgcagccaca cgccgaggcc aggctggtcg
      661 gcgcaggttg cgccgccgag cgcggtcgcg agctgggtga tgctctcgcc gctggcgcgt
      721 gaagaataca cgctatcggc ccgcgttcag gacctggcgc agcgcatgct gtccgcccgc
      781 gtccacgggg caagcgccgt gttcgtgccg ctgccgtttg acgccagtca cggcctgatc
      841 aatgccgatg gttcgccggg agagctgttc gtgccctggc ggacaacggc gacccttgtt
      901 ggcggcgccg agtacctggg atcaattcaa cttcccggcg gcagcacgtg ccacgcattc
      961 gggcgcgatg ggcaggcggt gctggccatc tggaacgagc agccgacgac cgagcggctg
     1021 acgctggacg acggcgtcga gcagatcgac gtttggggtc aaaacgtgct cctgccgatc
     1081 gaagcccagg ccgagccgca ggagcgcgaa atcgccgtcg ggccgctgcc gacatttctc
     1141 attgggcaga gcgaaggtgt ggcccgctgg caagcgacgg tggacttcga aagcctccag
     1201 gtcgccagcg tattcaacgt agaacagtcg attaacctca agattcgcaa tccgttcaac
     1261 cagggcatta gcggcgaagt gaagctgcag gcgcccgcga gctggggtgt cgatccgcgc
     1321 cccacgcgat tcaagatcgc ggtgggcgac gaattgcggc tggcgctgcc cgtgacgctg
     1381 cttgccgacg ccaacagcgg cccgcagccc gtgcggctcg atttcgagat cacggctgac
     1441 cgcaattacc gcttcagcgt ctatcgcacg ctgcagctcg ggctggacga cgtgcagatc
     1501 gagctccgca cgaagcttcg caacgacggc gcgctcatcg tcgaacagca actcaacaac
     1561 ctctccgagc ggcccgtcag ctttcagtgc ctgctgtttc cgcaggggcg gcggcgcgaa
     1621 atccggcagg tcatcaacgc ccagccgggc caaaccgcga cgacctacat cctcccccgc
     1681 ggcgaggagc tgatcggcca gcggctgctg ttgcgggcgg aagaaatcgg cggcccgcgc
     1741 gtgctgaact atacgctcac cggcgagcgg taagtggggc agacattcct gtctgcctca
     1801 gtgccagtct gtcgggaggc acgaatgcct gccccacgtg ttcctccgcc cgttgaagcg
     1861 aatgcgatcg cacagcacaa tgcaaagcaa gtcgtagccg cacgcaccag agtgcgggca
     1921 agcgcggcag cccgcgttct ggcgaacggg gctaccggcc tgcccgatcc ttcctttccc
     1981 gagagacgct caatgatccg cctattccaa aacgcgctct gctgcgttct tcccctgctg
     2041 ctggctgccg gactggcaaa ggccgacgag cccaagctga agctggtcaa aggcgaccgc
     2101 atcgtgatcg tcggcaacac gctggccgag cgcctgcagc agcacggcca ctttgagacg
     2161 ctgctgcaca gccggtttcc ggacctggag ctggtggtgc ggaatctggg ctggtcggca
     2221 gacgaaatct cacttcggcc gcgttccaag gacttcaagg accacggcca cacgttggcc
     2281 gaccacgatc cgaccgtgat cctggccttc ttcgggttca atgaatcgtt cgccggtccg
     2341 gcgggcctga gccagttcga agccgacctg gagagcttcc tcaaggcccc gcagaacatc
     2401 gaccgctatt ccacggcccg cagcaattgg gacaagacgc ccgacacggc ccaggagctg
     2461 ccgccgctga aatcgttgcg gcagatcgtg ctggtctcgc cgattgcctt caacgctctg
     2521 cacgactcgc atcatcccga cggcaacgtg caaaacaaga atctcaagct ctatagcgag
     2581 gtgatggccc gcatcgcggc gaagcacaag gtccctttcg tcgatctgtt tttgtccacg
     2641 ctgccgctga tggagtatcc gcgctatgcg actccgctca cgttcaacgg aattcacttg
     2701 aacgagaaag gcgatcaggc tgtttcgcga attctggatg tggcgctgtt tggtccgcgg
     2761 ccggaaaaca gggcgaacat cgacctggaa gtgctccgtc aggcagtcaa cgagaaggat
     2821 cgcctctttt ggctcgacta ccgcgccgtc aacggctttt atatctacgg cggccgcaaa
     2881 gagcccttcg gcgtcgtgaa tttcccggct gagttcgcca agctccggaa gatgatcgcc
     2941 aatcgcgatc agcgaatctg ggcggtcgct cagggcaaag aggtcccgcc gacgatcgac
     3001 gacggcaata cgggcgttaa tccaaagatc gaaacgaatt tcaaaaacct gatctcgatc
     3061 acgccgccgg aagaggccca gaagactttc acgctgcccg aaggctatgc gatcaactgc
     3121 tttgcctcag aagtcgattt tcccgatatg gaggatccgg tcgcgtttgc cttcgataac
     3181 aaggggcggc tgtgggtctg ctgcatgccg tcgtatccgc agtatctgcc ggggaagcag
     3241 atcaacgaca agattctgat cctggaagac agcgacggcg acggcaaggc cgacaagcag
     3301 acggtctttg ccgacaagct ctatctgccg gccgggatgg aattctggaa cggcggcgtg
     3361 attgtcgccc agcagccgaa tgtgctgttc ctcaaagata ccgacggcga cgacaaagcc
     3421 gacattcgcg agacgatcct gcacggcttt gattcggctg attcccacca tgcgatcagc
     3481 gcattcacgt tcgaccaggg cggggcgctc tatttccagg aggggacgtt ccaccattcg
     3541 cagatcgaaa cgcccttcgg cccgcggcgg tgcgtcaacg ccggcgtctt ccgcttcgag
     3601 ccgcggaccg acaagctcga cgtgttcatt tcctacagct tcgccaaccc gtgggggcac
     3661 atcttcgatc cctgggggca ggatttcgtg gcggatgcct cgcccggatt taattacttc
     3721 gcggccgcgt tctcgggcga cgtcgattac cccgacaagc acggctcgct caagcagatt
     3781 ctcgtcaagc agtggcggcc cacttgcggc tgcgagctgg tatccagccg caatttcccc
     3841 gacgaagccc agggcaacta tctgctcaac aactgcatcg gcttccaggg aacgctgcaa
     3901 tacaagctga aggacgacgg ggccggcttt gcggccgatc cggtcgatcc gctcttgcaa
     3961 tcgagcgatc cgagcttccg gccggtcgat atcgagttcg ccccggacgg gtcgctgttc
     4021 atctgcgact ggttcaatcc gctcatcggc cacatgcagc attcgatccg cgatccgaac
     4081 cgcgaccact cgcacggccg catctggcgg attcagtaca cgaaaaagcc gctcgtccgg
     4141 cccgtcaaga ttgacggcga gccgatcccg gcgcttctca agctgctcga aaccgagccg
     4201 gaggagcgga cgcgagcgcg agtgcgtgcc gagctatgga atcggccgac aaaggacgtg
     4261 ctggcggaag cgacaaagtg gctggccgcg caggacaagg ccggcaagga tttcgagcac
     4321 cacaagctcg aagcgctgtg gctgcatcag gcccacgact ccgtgaacga gccgctcctc
     4381 aagcagttgc tccgctcgcc cgactaccgg gcccgcgccg ctgcgacccg cgtgctttgc
     4441 tactggcgcg atcgcgtcac ccagccgctg gagctgctgc gggcgcaggt caacgacgat
     4501 c
//
LOCUS       DASUGB010000387         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1572899, whole genome shotgun sequence.
ACCESSION   DASUGB010000387 DASUGB010000000
VERSION     DASUGB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572899"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFV87_07425"
     CDS             <1..948
                     /locus_tag="VFV87_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103622.1"
                     /translation="YCLTDEARQKLQPKKLHEILAAMADKQLAAAREALSKAAPDERR
                     TKMQENWTRLLGDTTPAKDIKVREGAPHVEEVDGITITRELLETEPGIEVPVMTIELT
                     KFAKEKVRQPTLVVGLAADGLTGILQRQPELIAEGLSSDCIIVLPEVRGTGASHAGSG
                     QQGGMSSYSATSLMLGQPLLAGQLRDLRAVWKHMRGKHPRISSSMAVGGSGVEPLAAD
                     AAFSYPRRIDGRPPECQPTGALLAMLLGLYADVDSVVCRHGLASYGSVLDSQFVQVPH
                     ECIVPGVFQAGDLPALAEVLKIREFSVDLPVDGRGRLAE"
     gene            1003..3573
                     /locus_tag="VFV87_07430"
     CDS             1003..3573
                     /locus_tag="VFV87_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5103623.1"
                     /translation="MRGGLVSLTLVIIGLGPAAYRLLRADESPLPAPSQRQVDFRVDV
                     HPLLKARCFACHAGADCEAGVRLDLQAEILGETTGQPLVSVGKSGESRLIEVVAGMDE
                     KVVMPPKGERLTAGEIGLLRAWIDQGLAWESSLLPPLEAKSDHRAFQPVVQPPIVVGT
                     LRVASPDDGTRSAAATLNPIDAFTFARLTEAKLAPAPEADRRTLIRRLYTGTLGLLPA
                     AGEIDTFLADEAPDAYERLVDRVLASPHYGERIGRQWLDLARYADSEGYDSNHPRPFA
                     WLYRDYVVRAFNADKPYNEFVRQQLAGDEIEPYSDENLIATGFLAQARISSNEEDKWL
                     QRNQMLVEVVNATGQTFLGLTINCAQCHNHKFDPITARDYYRLLGFFISGQPTPIELP
                     RSPIRSVSEGVSPNLSDYGHALKLKQSIFETARTRRQASILEKLSDDERAALETPIAK
                     RTRAEELLARSVETRMQPSNSEVERHIPESDRKLYDELKKKVAELEKQGAALPQTWAF
                     YSPATSPHDLHVLPQIGFYPLPFERQELKQYRPYIAIRGDVHNIGPQVEPGWPAVLGR
                     ESDNPQSAIRNPQSRLRLAAWLTDRAHPLTARVWVNRLWQHHFGTGIVATSGDFGVQG
                     DPPSHPELLDWLAAELMERGWSTKRIERQILTSHTYRQSSVPNPQSAIRNPQSVDPDG
                     RLLWRFPARRLEAETLRDSLLTASGELDGQIGGPSLQEPDNPSAVRRSIYLLQKRGVP
                     PQMQKLFDGPTEMAESCTRRHISTTALQSLYLLNSPFALKQAEAIAALIRQAAGDDQE
                     AQIAAAFTRILGRAPDDDERQDAADFLAAQIGPDDGAADGLVLLCQTLLNTNEFCYVE
                     "
     gene            3579..4994
                     /locus_tag="VFV87_07435"
     CDS             3579..4994
                     /locus_tag="VFV87_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103624.1"
                     /translation="MSSNPQSAIHHPQYSRRTMLAGCSAGIGTLALASLLAEERLLAA
                     GSNGEPVAPSPRATAKRVIFLFMGGGPSQVDTFDPKPLLNELHGKSVPESIAASIPRI
                     ARAPLENLSGSKFKFQRHGQSGLEISELFPHVARCADDLCVLRSLKHDSPIHAPAEFI
                     ATTGTGIGDRPSLGAWLYYGLGSENRDLPGYIVMKSGETLRPPTIAAGFLPARYQGTL
                     VDGETGIPNLETPDGTSARQRRGQLDLLRRLNEKHLSRHGGDSALEARIQAYELSYRM
                     QMAAPEAFDLSQETAETHALYGLESKDTAEFGKYCLWARRLVERGVRFITLRSGGWDA
                     HESVPENHGKQCLATDQPIAALLTDLKRRGLFSETLVIWGGEFGRTPAAQGSKGGRDH
                     SPSGYSMWLAGGGVRGGQVIGATDPVGYAAIERPIHPNDLHATILHALGIDQRELSYL
                     HHNRREMVTVNGGDVIGEVFG"
     gene            complement(5000..7336)
                     /locus_tag="VFV87_07440"
     CDS             complement(5000..7336)
                     /locus_tag="VFV87_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103625.1"
                     /translation="MAHRWTNYLRKNTKPIMAVMAVVCMITFVVGFSLQRMIENYMRR
                     KETKDPVVVTWAKGSVHESDLDLMRRRHGVAVRFVAGVIERAVQAGGSPIIGGQPAID
                     PRTGNIVQIGFSEDGSDEGLIRTMVLAEEARRMGIAVDPGAVKTFLYELSGDVLQEAD
                     WEEVAMEVTEDGQMLSMTDLFAQVAYELRAQQVRVLMRAGLLAIPPGEVWDYFNRLNR
                     QFSIEAYPIDVKPLVAQVGGEPTGTEIRELFEKGQARDPNPNDADPGFHEPLRIAFGY
                     VKVDFAPFLEKAKTQITDEQVEEQYKKDIEQGLHKVPELPALPPSGTEGEKPADGDKP
                     AEGDKPAEGDKPADSDNPAEGEKKEADKPAEANKETEPAKPEEKGADKPYGSSGCQDP
                     PAAAKPADEKPADEKPVEKPAEATAEVKPADAAKPAEEKPADETKPADAAPPADPAKE
                     PKFKSLEEVKDDIRTRLAQPIAQEAQTAAVKELTDAIRSYGKAYYRWEAAKNQKRKPE
                     SLKDPGALKLNELAARYGFEAGTIPLVDRFTVRDYELGQKVSVFNFQLMQMGHSPLLS
                     FSDIAFGENVPLYDPQQAESSEPDISYVFFRTEVKEPRDLTLAEAREQVVEAWKMNKA
                     LELALQDAQKLADKAKGAKSLKDVVSDPAKVIQPLPFSWMTTGSLAFGFGQPSISQVD
                     GIEFAGDEFMRAVFALAPGEAGVAPNAAHKRVYVVRVVSQTPQDDLLRQQFLDSGFSG
                     VGMIASREVGEILFQMYENLEDRMNVHWERPPQMEGRM"
BASE COUNT         1312 a         2443 c         2325 g         1329 t
ORIGIN      
        1 tactgtctga ccgacgaagc ccgccagaag ctgcagccga aaaagctgca cgagattctc
       61 gccgctatgg ccgacaagca gcttgccgcg gcccgcgagg ctttgtccaa ggcggcgccg
      121 gacgagcggc ggacgaagat gcaggagaac tggacgcgct tgctcggcga tacaacgccg
      181 gccaaagaca tcaaggtccg cgagggtgcg ccgcacgtcg aggaggtgga cggaatcacg
      241 atcacgcgcg aactgctgga aacggagccg gggatcgagg tcccggtgat gacgatcgag
      301 ctaaccaaat ttgccaagga aaaggtacga cagccgacct tggtcgtggg actggcagcc
      361 gacggcttga cgggcattct gcagcgccag ccggagttga tcgccgaagg gctgagttcc
      421 gattgcatca tcgtcttgcc ggaggtacgc ggaacgggcg catcgcacgc cggcagcgga
      481 cagcagggcg ggatgagctc ctattcggcg acgagcctga tgctgggcca gccgctgttg
      541 gccggccagt tgcgcgactt gagagccgtg tggaaacaca tgcgcgggaa acatccgcgc
      601 atctcgtcgt cgatggctgt cggcggttcg ggcgtcgagc cgctggcggc cgacgcggca
      661 ttcagctatc cgcggcgaat cgatggccgg ccgccggaat gccagcccac gggcgcgctg
      721 ctcgcaatgc tgctcggcct ttacgcggac gtggattcag tcgtctgccg gcatggcctg
      781 gcgtcgtatg gctcggtgtt agacagccaa ttcgtgcaag tcccgcacga atgcatcgtg
      841 cccggcgtgt ttcaggcagg cgaccttccg gcgctggccg aagtgctgaa gattcgcgaa
      901 ttcagcgtgg atttgccggt ggacggccgt gggcggctgg cggagtaatc gtgggatagg
      961 cttcattgct gctgctaaaa taggtagagg cgaggagatg cggtgcgagg cggacttgtc
     1021 agtttgacgc tggtcatcat aggccttggg ccggcagcgt atcgtctctt gcgcgccgac
     1081 gaatctccgc tcccggcgcc gagccaacgg caggtcgatt tccgcgttga cgtccacccg
     1141 ctgctcaagg ctcgctgttt cgcctgccac gcgggcgccg actgtgaagc gggcgtgcgg
     1201 ctcgatttgc aggcggagat cctgggcgaa accaccggcc agccgctcgt ctccgtcggc
     1261 aaaagcggcg aaagccggct gatcgaggtc gtcgccggaa tggacgaaaa ggtcgtcatg
     1321 ccgcccaagg gcgagcggct gacggctggc gaaattggtc tgctccgggc gtggatcgac
     1381 cagggcctgg cgtgggagag cagcctgctg ccgccgctgg aggcgaagtc ggaccaccgg
     1441 gcgtttcagc cggtggtgca accgccgatc gtagtaggca cgctccgtgt ggcgtcgcct
     1501 gatgacggca cacggagtgc ggctgctact ctcaatccga tcgatgcctt cacttttgct
     1561 cgcctcacag aagcgaagct cgcgcccgcg cccgaagccg accgccgcac actcatccgc
     1621 cgcctttaca cgggcacgct cggcctgctg ccggctgccg gcgaaatcga tacgttcctg
     1681 gcggatgaag cgcccgacgc ctacgagcgg ctggtcgatc gggtgctggc cagcccgcac
     1741 tatggcgagc gaatcggccg gcaatggctc gatctggccc gctatgccga cagcgagggc
     1801 tacgatagca atcacccgcg gccgttcgcg tggctgtacc gcgactatgt cgtgcgggcg
     1861 ttcaacgccg acaagcccta caacgaattc gtccgccagc aacttgccgg agacgagatc
     1921 gagccgtact cggacgagaa tctgatcgcc acgggcttcc tggcccaggc ccgcatcagc
     1981 agcaacgaag aagacaagtg gctgcagcga aatcagatgc tggtcgaagt cgtcaacgcc
     2041 acgggccaga cgtttctcgg gctgacgatc aactgcgccc agtgccacaa tcacaagttc
     2101 gatccaatca ccgcccgcga ttattaccgc ctgctcggct tcttcatcag cggccagccg
     2161 acgccaatcg agctgccgcg atcaccaatc cgaagcgtaa gcgagggagt ctcgccaaat
     2221 ctgagcgact acggccacgc tctcaagctc aaacaatcga tcttcgaaac agcccgcacc
     2281 cgtcgacaag cgtcaattct cgaaaagctt tcggatgacg agcgcgccgc gctggaaacg
     2341 cctatcgcca agcgcacgcg agccgaagag ctgctcgccc gtagcgtcga aacccgcatg
     2401 cagccctcca acagcgaggt agagcggcac atccctgagt ccgaccgcaa gctctatgac
     2461 gagctgaaga agaaagtcgc cgagctggaa aaacaaggtg cggcgctgcc gcaaacctgg
     2521 gccttctatt cgcccgcaac cagcccgcac gatttgcacg tgttgccgca aatcggcttt
     2581 tacccgctgc cgttcgagcg ccaggagctg aagcaatacc ggccgtacat cgccatccgc
     2641 ggcgacgtcc acaatatcgg cccgcaggtc gagcccggct ggccggcggt gttggggcgg
     2701 gagtcagata atccgcagtc cgcaatccga aatccgcaat cgcggctccg cctggccgcc
     2761 tggctcaccg accgcgcgca cccgctgacg gcccgcgtgt gggtcaaccg gctgtggcag
     2821 catcacttcg gcacgggaat cgtcgcgacc agcggcgatt tcggcgtcca gggcgatccg
     2881 ccctcgcacc cggaattgct cgactggctg gccgcggagc tgatggagag aggctggagc
     2941 accaagcgca ttgagcggca gattctcacg tctcacacct accggcaatc gtccgttccc
     3001 aatccgcaat ccgcaatccg caatccgcaa tccgtcgatc ccgatggtcg cctgctgtgg
     3061 cgctttccgg cgcggcggct ggaggcggag acgctgcgcg attcgctgct gaccgccagc
     3121 ggagagctgg acgggcagat cggcggaccg agcctccagg agccggacaa tccatcagcc
     3181 gtgcggcgaa gcatctacct gctgcagaag cgcggcgtcc cgccgcagat gcagaagctc
     3241 ttcgacggcc cgaccgaaat ggccgaaagc tgcacgcggc ggcacatctc gacgacggct
     3301 ctgcagtcgc tctatctgct gaacagcccg tttgccctga agcaggccga agcgatcgcg
     3361 gcactcatcc ggcaagcggc aggcgacgac caggaggcgc aaatcgcagc cgccttcacg
     3421 cggattcttg gccgcgcgcc ggatgacgac gaacgccagg acgcagccga cttcctggcc
     3481 gcgcagatcg gaccagacga cggcgccgcg gatgggctgg tgctgctgtg ccagacgcta
     3541 ttgaatacga acgaattctg ctacgtggag tgattcccat gtcctccaat ccgcaatccg
     3601 caatccacca tccgcaatat agccgacgta ccatgctcgc cggttgctcc gccgggatcg
     3661 gcacgctggc gcttgccagc ctgttggccg aagaacgttt gctggcggct ggatcgaacg
     3721 gtgagcccgt cgcgccgtcg ccccgcgcca ccgccaagcg cgtcatcttt ctcttcatgg
     3781 gcggcgggcc aagccaggtc gatacgttcg accccaagcc gctgttgaac gaattgcacg
     3841 gcaagtcggt gccggaatcg atcgcggcct ccattccgcg aatcgcccgc gctccgttgg
     3901 aaaacctgag cggcagcaag ttcaagttcc agcggcacgg ccaaagcggc ctggaaatct
     3961 cggagctgtt cccgcacgtc gcccgctgcg ccgacgacct gtgcgtcctc cgcagcctga
     4021 agcacgacag cccgatccac gccccggccg agttcatcgc caccacgggc accggcatcg
     4081 gcgaccgtcc aagcctcggc gcgtggctct attacggcct gggaagcgag aaccgcgatc
     4141 tgcctggtta catcgtcatg aaatcgggcg aaacgctccg cccgccgacg atcgccgcgg
     4201 gctttctgcc cgcccggtat caggggacgc tggtcgatgg tgaaaccggc atcccgaacc
     4261 tggaaactcc agacggcact tccgccaggc agcgccgcgg gcagcttgat ttgctgcggc
     4321 gtctgaacga gaagcacctc tcgcggcatg gcggcgacag cgcgctcgag gcccggatcc
     4381 aggcctacga gctctcgtac cgtatgcaga tggccgctcc ggaagccttc gacctctccc
     4441 aggaaacggc ggagacgcat gcgctttacg gcttggagag caaggatacg gctgaattcg
     4501 gcaaatactg cctgtgggcg cggcggctgg tcgagcgcgg cgtgcggttc atcacgcttc
     4561 gctccggcgg ctgggacgcc cacgaaagcg tccccgagaa ccacggcaag cagtgcctgg
     4621 cgaccgacca gccgatcgcg gccctgctca ccgatctgaa gcgccgcggc ctattctccg
     4681 agacgctcgt catctggggc ggcgaattcg gccgcacgcc cgccgcgcaa gggagcaaag
     4741 gaggccgcga tcattcccct tcgggctaca gcatgtggct ggccggcggc ggtgtccgcg
     4801 gcgggcaggt gatcggcgcg accgatccgg tcggctatgc ggcgatcgag cggccgattc
     4861 accccaacga cctgcacgcc acgatcttgc acgccctggg catcgatcag cgcgaattgt
     4921 cttacctgca ccacaaccgc cgcgagatgg tgacggtcaa cggcggcgac gtgatcggcg
     4981 aagtcttcgg ctgagcgggt tacatccggc cttccatctg cggcggacgt tcccagtgaa
     5041 cgttcatccg atcttccaga ttctcataca tctggaacaa gatctcgccc acctcgcgac
     5101 tggcgatcat accgacgccg gagaagccgc tgtcgaggaa ctgctgccgc aagaggtcgt
     5161 cctggggagt ctgggacacg acgcggacga catagacccg cttgtgcgcc gcgttgggtg
     5221 cgacgcccgc ctcgccgggc gccagggcga acacggcccg catgaactcg tcgccagcga
     5281 attcaatgcc gtccacctgg ctgatgctcg gctggccgaa gccaaacgcc agcgagccgg
     5341 tcgtcatcca cgagaacggc agcggctgga tcaccttcgc cggatcgctc acaacgtctt
     5401 tcagcgactt cgcgcctttg gccttgtcgg ccagcttctg ggcgtcctgc agggccagct
     5461 ccagagcctt gttcattttc caggcttcca caacctgctc gcgcgcctcg gccagcgtca
     5521 ggtcgcgcgg ctctttgacc tcggtgcgga aaaagacgta gctgatgtcc ggctcgctgg
     5581 attcagcttg ctgcgggtca tacagcggca cgttctcgcc gaaggcaata tcggaaaacg
     5641 acaagagcgg tgagtgcccc atttgcatga gctggaaatt gaacacggag actttttggc
     5701 cgagctcgta gtcgcggacg gtaaagcggt cgacgagcgg aatcgtgccc gcttcgaagc
     5761 cgtacctggc cgccaactca ttgagtttca gcgcgccagg atctttcagc gactcaggct
     5821 tgcgcttctg gttcttcgcc gcctcccagc ggtaataagc cttgccgtag gaccgaatcg
     5881 catccgtcag ctctttgacg gccgcggtct gggcttcctg ggcaatcggc tgggccaggc
     5941 gggtgcggat gtcgtccttg acctcctcca gcgacttgaa cttgggctct ttggccggat
     6001 cggcaggcgg ggctgcgtct gcgggcttgg tttcgtccgc aggcttttct tcggcgggct
     6061 tcgcagcgtc ggcgggtttg acttcggcgg tcgcttcggc aggtttttcc acaggcttct
     6121 cgtcggcagg cttctcgtcg gccggcttcg ccgcggcagg cggatcctgg cagccggagg
     6181 agccgtatgg cttgtcagcg cccttttcct ccggtttggc aggctcggtt tccttgttcg
     6241 cttcggccgg cttgtcggct tccttctttt cgccttcggc gggattgtcg ctgtcggcgg
     6301 gcttgtctcc ctccgcgggc ttgtctccct ccgcaggctt gtcgccgtcg gccggttttt
     6361 cgccttcggt gccggatggc ggcaaggccg gcagttcagg aaccttgtgc aggccctgct
     6421 cgatgtcctt cttgtactgc tcctccactt gctcgtcggt gatttgcgtt tttgccttct
     6481 ccaggaacgg ggcgaaatcc acctttacgt agccaaacgc gatccgcaga ggctcgtgaa
     6541 agccggggtc ggcgtcattc ggattggggt cgcgggcctg gcccttctcg aacagctcac
     6601 ggatttccgt cccggtcggc tcgcctccga cttgggccac gagcggcttg acgtcgatgg
     6661 gatacgcctc aatcgagaat tgccgattga gccggttgaa gtagtcccac acctcgccgg
     6721 gcggaatcgc gagcaggccg gcccgcatca gcacgcgcac ctgctgcgcc cgcaattcat
     6781 aggcgacctg agcgaacaga tcggtcatgc tgagcatctg gccgtcttcg gtcacctcca
     6841 tggcgacctc ctcccagtcg gcttcctgaa ggacgtcacc gctcagctcg tacaaaaatg
     6901 tcttcacggc gcctggatcg acggcgattc ccatccgccg cgcctcttcg gccagcacca
     6961 tcgtcctaat caggccctcg tcgctaccat cttccgaaaa gccaatttgt acgatattgc
     7021 ccgtccgcgg gtcgatcgcc ggttgcccgc cgatgatggg cgaaccgccc gcttgcactg
     7081 cccgctcaat gacgcccgcg acgaatcgga ccgccacgcc gtgccgcctg cgcatcaggt
     7141 cgaggtccga ctcgtggacc gatcccttcg cccaggtgac gacaaccgga tccttcgtct
     7201 ccttgcgccg catgtagttc tcgatcatcc gctgcagcga gaacccgacc acgaacgtga
     7261 tcatgcagac gacggccatc acggccatga tcggcttcgt gttcttgcgg agatagttcg
     7321 tccaacgatg tgccatgacg gcccttcctt cgggggttct gccgtagtcc cgttgacaga
     7381 ccacccaccc tgcgcttatt gacttagac
//
LOCUS       DASUGB010000388         6057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1431700, whole genome shotgun sequence.
ACCESSION   DASUGB010000388 DASUGB010000000
VERSION     DASUGB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431700"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VFV87_07445"
     CDS             complement(<1..1577)
                     /locus_tag="VFV87_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103626.1"
                     /translation="MRIALLITSLLTAITVSASHAEDLALEPINVGTPSKIEVFPAAI
                     KIAGARRHMQLVVTGHYADGTLQDLTRAAQFATSNAAVAVVDNAVAQPKGDGAAEIVV
                     TVGGQEAKVPVEVSAFATPQPISFEFETLAALSKQGCNSGACHGSPSGKGGFRLSLRA
                     FDPSIDKLTLIREDLGRRTNPLSPEESLLLNKPLMKVPHGGGLKLKKSEPAFALLRQW
                     IAEGCQPDKADAPRCVKIEVYPPSGRVLKRPAHTQQLAVLAHFADGTTRDVTDMACYS
                     SSDSMVAEVNEQGLVVGHDRGEAAIIIRYLEHVESCFLTFVKDIESFAWNNPPTINYI
                     DEHVDAKLQQLKYLPSGTCSDEEFLRRVHLDVIGQLPTLEETQAFLADGAPDKRAKLI
                     DNLLDRPEYARFFALKWGDLLRLTTTQIGGPGAFKYHRWVQRAFESNMPYDQFAHELL
                     AATGSTLNNPAANFYRTATDTQDCVESISQIFLGARLQCAKCHNHPFEKWTQDNYYGM
                     AAFFNRVQRKKSPRADE"
     gene            complement(1658..3997)
                     /locus_tag="VFV87_07450"
     CDS             complement(1658..3997)
                     /locus_tag="VFV87_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5103627.1"
                     /translation="MFRLLPRRSHAVWLVLASQLLLLVASQQALAQPTLSAAVPGAVA
                     PGKTTDVTLSGAKLDAPLKVWTSLPAQIELVPGDPNQKDKTSLVCKITLPAGAPLGIG
                     GLVLADDKGISEPLLIMVDDLPSVLDNSGNHDLGSAQEVQLPIGIDGYCDGTTFDYYR
                     FTAKAGQRIAGEVLATRLGWDFDPVVRLLDAAGGEVLRADDDPASGADSRFAFTAPAD
                     GQYVLELRDNRYKAGGRYRVRLGDLPLVSTTSPLAVAAGIPSAVGVVGPLTEGVTPLT
                     ILTPVSAGGQMIDLSARASSSQAGSFATLYASDLPVFGETAPADKPDEASAAAIPGVM
                     TGILEAPKDRDLFQFTATKGTPVSFRSITKSAGSSAILMLRLLNAAGAQVAESPINDS
                     DEPVLQFTIPEDGAYKLAIEELAGRGDVDYTYAVEARTGPQFTLQLKADPNQNRLKHF
                     LPGGDGALHLDVQCTRFAYDGPITLDVESNHAGWQVFSNVIPAKANEVRLYVVTPLDL
                     APADLAAIRIVGRADESGRGYSARMATTQQLRTARPYVPYPAAWLDGVIFVSGIADKA
                     PFHRVTPDRLDVNFPRLVGQTQFTLKFERLVEAFKDVPLTVLPLGLPPGVSAEVKRNG
                     NGPSETYDIILKGPKDLAEGLHTIRYFAFTEMGANGRGTQSGDLRLNVITPLAVVAAP
                     AGPIVQGQKQKVKLTLTRKGDDKQPVDVVFKALPAGVTAPEKTTLAADQNEIEAELSA
                     AADAAVVKFEQLVVTATSKYAGTDIGVDSPAAVLEVKAP"
     gene            complement(4205..4277)
                     /locus_tag="VFV87_07455"
     tRNA            complement(4205..4277)
                     /locus_tag="VFV87_07455"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4242..4244),aa:Ala,seq:cgc)
     gene            4551..5366
                     /locus_tag="VFV87_07460"
     CDS             4551..5366
                     /locus_tag="VFV87_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5103628.1"
                     /translation="MARLAINELTTFRWSLEEDLEHYAAAGIGAIGVWRQKLADCGEE
                     MGARLLAESGLAISSLQWAGGFTGSEGPSHGESLADARQALRLAANLHADCLIIHSGA
                     RGIHTHNHARRLFRQALDKLLPIAEDWGVTLAVEPMQGDCGSEWTFLNCLDETLELVA
                     GYDSSALKIVLDTYHWASGPALFDRLPRMLPRLALVQLGDARQPPAGEPNRCPLGKGV
                     LPLKQIVSRLIATGYEGHFEVELMGEEIEACDYHELLAESVRTFDDWAAAAAR"
     gene            complement(5396..5605)
                     /locus_tag="VFV87_07465"
     CDS             complement(5396..5605)
                     /locus_tag="VFV87_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103629.1"
                     /translation="MRFLCTIEPLPAGGFAASHKSREVGAVRIQAPTRDEAIEKVKGE
                     IRYRLELCPCTGETYQHIAVEIVEK"
     gene            complement(5616..>6057)
                     /locus_tag="VFV87_07470"
     CDS             complement(5616..>6057)
                     /locus_tag="VFV87_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5103630.1"
                     /translation="GMAHYRAMCLQCHGAPDVKSHAFAKGLNPPPPALAHVAREWSDG
                     ELFWITKHGIRMTGMPAFGHNHSDEELWKIVAFLRQLDDLSEEQVAMLREGEEAGHDH
                     GDHEHAGGHDHELVTGHEKASGEQPEAEGEERVAEKPDPETQER"
BASE COUNT         1053 a         1936 c         1915 g         1153 t
ORIGIN      
        1 atctcgtcgg cccgcggtga tttcttgcgc tgcacacgat tgaagaacgc tgccatgcca
       61 taataattgt cctgcgtcca tttctcgaac ggatggttgt ggcactttgc acattgcagg
      121 cgggcgccga ggaagatctg cgagatcgac tcgacgcagt cctgtgtgtc ggtcgccgtg
      181 cggtagaagt tcgccgccgg attgttcagc gtgctgccgg tggcggccaa gagctcgtgg
      241 gcgaactgat cgtagggcat gttcgactcg aacgcccgct gcacccagcg atggtacttg
      301 aacgcacccg gtccgccgat ttgcgttgtc gtcaggcgga gcaagtcacc ccacttgagg
      361 gcaaagaacc gggcgtattc cggccggtcg agcaggttgt cgatcagctt ggcccgcttg
      421 tcgggtgcgc cgtcggccag gaaagcttgc gtctcttcga gcgtcggaag ctggccgatc
      481 acgtccaggt gcacgcggcg gaggaactct tcgtcgctgc aggtgccgga aggcagatac
      541 ttgagctgct gcagcttggc atcgacgtgc tcgtcgatgt agttgattgt cggcggatta
      601 ttccaggcga aactctcgat gtccttcaca aacgtcagaa agcagctctc gacatgctcc
      661 aaataacgga tgataatcgc cgcttcgccg cggtcgtgac cgacgaccag accctgctcg
      721 ttcacctccg ccaccatact gtcggaactg gagtaacagg ccatgtcggt gacgtcgcgg
      781 gtcgtgccgt cggcaaagtg ggccagcacg gcaagctgct gggtgtgggc tggccgcttc
      841 aacacgcggc cgctcggcgg atagacctcg attttgacgc accgcggcgc gtccgccttg
      901 tcgggctggc acccctcggc gatccactgc cgcaagaggg cgaaagccgg ctcgctcttt
      961 ttcagcttca ggccaccgcc gtgcggcact ttcatcagcg gcttattgag gagcaggctt
     1021 tcctcgggac tgagcgggtt ggtgcggcgg ccgaggtctt cgcggatgag cgtcagtttg
     1081 tcgatcgacg gatcgaaggc ccgcagcgac aatcgaaagc cccctttccc gctgggcgag
     1141 ccatggcaag cgccgctgtt gcagccctgt ttggacaggg cggcgagcgt ctcgaattcg
     1201 aatgagatag gctgcggagt tgcgaatgcg ctgacttcaa ccggcacttt cgcttcctgg
     1261 ccgccgacgg tgacgacgat ctccgccgca ccgtccccct tgggttgtgc aacggcgtta
     1321 tcaaccaccg cgaccgcggc gttgctggtc gcaaactggg cggcgcgcgt caggtcttgc
     1381 agcgtgccat cggcatagtg gccggtgacg acgagctgca tgtggcggcg agcgccggca
     1441 atcttgatcg ccgcggggaa gacttcgatc ttcgatggcg tgccgacgtt gattggctca
     1501 agagccagat cttcggcgtg gctcgccgac accgtgatcg cagtcaacaa cgaagtgata
     1561 agcagcgcga ttcgcatatc gaggtccttt caatggttcg ctgcgttcgg ttctcgttct
     1621 cagtgtgagg gaagcgcgac cactggtcgc ggctatgtca gggcgccttc acttccagca
     1681 cggcggccgg gctatcgacg ccaatatcgg tcccggcata tttactggtg gcagtgacca
     1741 cgagctgctc gaacttgacg accgcggcat cggcagccgc ggaaagctca gcttcgatct
     1801 cattttgatc ggcggcgagc gttgtcttct caggtgcggt gacgccggcc ggtaaggcct
     1861 tgaagaccac gtccacgggc tgcttgtcgt cgcccttgcg ggtgagcgtc agcttcactt
     1921 tctgtttttg tccctgcacg attggtcccg ctggagcggc gacgacagcg agcggcgtga
     1981 ttacgttcag ccggagatcg cccgactgcg tgccgcggcc attggcgccc atctccgtga
     2041 acgcgaaata gcggatcgtg tgcagtccct cggccagatc tttcggaccc ttgagaatga
     2101 tgtcgtacgt ctcgctcggc ccgttgccgt ttcgcttgac ctcagccgaa actccgggcg
     2161 gcaggccgag cggcaggaca gtcagcggca cgtccttgaa cgcctcgacc agccgttcga
     2221 acttgagcgt gaactgcgtc tgccccacca gccgcgggaa gttcacatcc aggcggtcgg
     2281 gcgtgacgcg atggaacggc gctttgtcgg cgatgcccga gacgaaaatc acgccgtcga
     2341 gccaggccgc ggggtatggc acatagggcc gtgccgtccg aagctgctgg gttgttgcca
     2401 tccgtgccga atagccccgg ccgctttcgt cggcccggcc cacgatgcga atcgccgcca
     2461 gatcggccgg cgccagatcg agcggcgtca cgacatacag ccgaacttca ttcgccttgg
     2521 cgggaatcac attgctaaag acctgccagc cagcgtgatt cgattcaacg tcgagcgtga
     2581 tcggcccatc gtaagcgaac cgcgtgcatt gcacatccag gtgcagcgcc ccatcgccac
     2641 ctgggaggaa gtgcttgagg cggttctggt tcggatcggc tttgagctgc agcgtgaact
     2701 gcggccccgt gcgggcttcg acagcgtagg tgtaatcgac gtcgccgcgg cccgcgagtt
     2761 cttcgatggc gagcttgtac gcaccgtcct ctgggatcgt gaattgcagc accggctcgt
     2821 cgctgtcgtt gatcggactc tctgcgacct gcgcgccagc cgcattcaga aggcgaagca
     2881 tcaggatcgc gctggagccg gcgctcttcg taatcgaacg gaagctgacg ggggtgcctt
     2941 tcgtcgccgt gaactggaac agatcgcggt ccttgggcgc ttcgaggatg cctgtcatca
     3001 cgcccggaat cgccgcggcg ctcgcttcgt cgggcttgtc ggctggggcg gtctcgccga
     3061 agacgggcag atcgctggcg taaagcgtgg caaagctgcc agcctgactg ctggaagcgc
     3121 gggcgctgag gtcgatcatt tgtccgcccg cggaaactgg ggtcaagatc gtgagcggcg
     3181 tgacgccttc ggtcagcgga ccgacaacgc ccacggcact cggtatgcca gccgccactg
     3241 ccagcggcga cgtcgtgctg acgagcggca aatcgccgag ccgcacccgg tagcgcccgc
     3301 ccgctttata gcggttgtcg cgcagctcca gcacatactg cccatcggcg ggcgcagtga
     3361 aggcaaaccg cgaatcagcg cccgaggccg ggtcgtcgtc ggcccgcagc acttctccgc
     3421 ccgccgcatc gagcagccga accaccggat caaaatccca gcctaggcgc gttgcgagca
     3481 cttcgccggc aattcgctgc ccggccttgg ccgtaaagcg gtaataatcg aacgtcgttc
     3541 cgtcgcaata cccgtcgatg ccgattggaa gctgtacttc ctgagcactg cccaagtcgt
     3601 gattgccgct gttatccaag acacttggca gatcatcgac cataatcagg agcggctcgc
     3661 tgatcccttt gtcgtcggcc agaacgagtc cgccgatgcc taagggcgcc ccggcgggca
     3721 gcgtgatttt gcaaaccagc gaggtcttgt ccttctgatt cggatcgccc ggcaccaact
     3781 cgatctgcgc gggcaagctc gtccatacct tcagcggtgc gtctagtttc gccccgctca
     3841 gcgtcacgtc agtcgtcttg ccgggcgcga ccgccccggg aacggcagcg gacaacgtgg
     3901 gctgggcgag ggcttgctga ctggcaacca gcaacagcaa ttggctcgct agcaccaacc
     3961 acacggcgtg cgatcgcctg gggaggaggc gaaacatgcg gcaagtcctt ggcaggcagg
     4021 atgttggcgg gaccgcagcc gggttgttca cctgggccgg ggcggaatcc agccgatggc
     4081 ttaaccatcg acgctggcta atgaatcccc attctggatc gagccagtcg ccgggtcaac
     4141 caacgggacc agaatgtgcc gcaagaaggg cctctcacgg gacaagaccc ctacagcaag
     4201 aaagtggagc ggaagggagt tgaaccctcg acctctgcat tgcgaacgca gcgctctccc
     4261 aactgagcta ccgccccagg cacctgcact acttgtgcgt ctggaatata acgcgccggc
     4321 tggagaacgg ctagagtgag ccgccagcta gcagttcggc gcgacttgcc gcaaatcgcg
     4381 ccgtcgagcc agcttagggc cgggcagcag gcgtcgtttg ctgccggcag agacgcggcc
     4441 tgcgcgccga atgttcgcag ttcctttaca gaatctgcaa gctgcgacat actgcccccc
     4501 tcgcctgcgg ccgcttggaa ctggccggcg aagcaacgga aggaacaggg atggctcggc
     4561 tggcaatcaa tgaactcacg acctttcgct ggtcgctcga agaggatctg gaacactacg
     4621 cggcggcagg catcggcgcc atcggcgtct ggcggcagaa gctggccgat tgcggcgagg
     4681 aaatgggagc caggttgctc gccgaatcgg gactcgcaat ctccagcctg cagtgggccg
     4741 gcggcttcac tggcagcgaa ggtcccagcc acggcgaaag cctggccgac gcccgccagg
     4801 cccttcgcct ggctgccaac ctgcatgccg attgtctgat catccacagc ggcgctcgcg
     4861 gcattcatac gcacaatcat gcccgccgcc tcttccggca agcgctcgac aagctcctgc
     4921 ccatcgccga ggactggggt gtaacattgg ccgtcgagcc gatgcaaggc gattgcggga
     4981 gcgagtggac ctttctcaac tgcctggatg aaactctcga actcgtcgcc ggctacgatt
     5041 cgtcggctct caagatcgtg ctcgatacct accactgggc cagcgggccg gctctcttcg
     5101 accgcttacc gcggatgctg ccgcggctgg cgctggtgca gcttggcgac gcgcgccagc
     5161 cccccgccgg cgagccgaac cgctgcccgc tgggcaaggg cgtgctcccg ctcaagcaga
     5221 tcgtttcgcg cctcattgct accggatacg aggggcactt cgaggtcgag ctgatgggcg
     5281 aagagattga agcctgcgac taccacgaat tgcttgccga aagcgtccgc acgttcgacg
     5341 actgggcagc cgctgccgcc aggtagcagg cacactccgt gtgccgtccc ggcagtcact
     5401 tctccacgat ctcgactgcg atgtgctgat acgtctcgcc ggtgcaggga cacaattcca
     5461 ggcgataccg aatctcgcct ttcaccttct cgatcgcttc gtcccgcgtc ggggcctgga
     5521 ttcgcaccgc gcccacctcg cggctcttat ggctcgccgc aaacccgccc gccggcaacg
     5581 gctcgatggt gcagagaaaa cgcattggcg taattctacc gctcctgcgt ctctggatca
     5641 ggcttttccg ccacgcgctc ctcgccttcg gcctccggct gctcgccact agccttctcg
     5701 tggccggtga ccagttcgtg gtcgtgaccg ccagcgtgtt cgtggtcgcc gtggtcatgg
     5761 ccggcttctt cgccctctcg caacatggcg acctgctctt ccgagagatc atcaagctgc
     5821 cgcaggaacg cgacgatctt ccacagttcc tcgtcgctgt ggttgtgccc gaacgccggc
     5881 atgccggtca tgcggatgcc gtgcttggtg atccagaaca gctcgccatc cgaccattcg
     5941 cgcgcgacat gggcgagcgc gggcggcggc ggattgagcc ctttcgcgaa cgcgtgggat
     6001 ttcacatccg gcgctccgtg gcactgcagg cacatcgccc ggtaatgggc catccct
//
LOCUS       DASUGB010000389         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1989041, whole genome shotgun sequence.
ACCESSION   DASUGB010000389 DASUGB010000000
VERSION     DASUGB010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989041"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..1035
                     /locus_tag="VFV87_07475"
     CDS             424..1035
                     /locus_tag="VFV87_07475"
                     /inference="COORDINATES: protein motif:HMM:NF037233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103631.1"
                     /translation="MSNWLSCHLLLVGPRPEIERFADAAVYKVPVADGEQESVPFALR
                     AICPLPDVPDCGPTTTRRVREYCWSTDCDVTACGDVERGDTNTSDEADSTTEYRFWSA
                     SAPPIRAILTASKRFDLEFQLRWIDRLGQTTGGEVTIRAGRFVDEQASPQIEYLFLDG
                     SELNRREAECAADYSVELCTDPGISSADKLMDALREQTHFHQL"
     gene            1046..1777
                     /locus_tag="VFV87_07480"
     CDS             1046..1777
                     /locus_tag="VFV87_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103632.1"
                     /translation="MSENCDFSVTVKGDYAQLEDLLETALGPSVTLVMGRFYLDPGSA
                     GVQIDEAASKQFVSLRDLRGDEQKYNALQLADWPCFNSFVGDWHILQTPRPMGEIDRI
                     NEFLDRSAAERLAMFDEFVKLVGLEYESSAALDASREALARSDWPRNRYRGLLDQPMP
                     RLLLWGESDWGPPLIFQALAKHWPLLVFVAGGSTDCELYEEYEWSGDSERCNYRLIVN
                     PRTDEVEWVVKDGVVISQAKWQPVR"
     gene            1842..2756
                     /locus_tag="VFV87_07485"
     CDS             1842..2756
                     /locus_tag="VFV87_07485"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX5103633.1"
                     /translation="MPILGAHQSIAGGFHRAVERAAAVGCETVQIFTAAPHQWPKNLP
                     LSYQKNSTKNNNQWRAKAITEAEAEQFAAALAERKIAHPLSHSSYLINLASPDAALRS
                     KSVESMIVELQRAAQLGIPYVVVHPGAHTTASLEEGLSLVAKSLDEIHAALPKTPAQI
                     LLEITAGQGSCLGHRFEHLAEILKQTKKPERVGICFDTCHAFAAGYDLRGAQSYKAMW
                     CAFDDLLGIGRLKAIHLNDSKRELGSRVDRHEHIGRGHIGAEGFRLLLRDRQLREIPM
                     YLETPKGDHEGETWDAINLRTLRELAKA"
BASE COUNT          564 a          826 c          849 g          567 t
ORIGIN      
        1 cggggccacg agagaaggtt tgagaaggta tcggcctagt gcggccagaa agtatttatc
       61 gttgtcatca caataatgaa aagtgcgctc tagctcaaga gcagatacgt caacaggcgg
      121 tcttgtgcta tacagcacaa ctcgcggcgt gtgggtgcgc acggattcac aatacacggc
      181 cactcgcata caatacttgc actggccgac tcccccgttg agatgcgaaa gctgtcgaac
      241 gatgtaatgg aggcgacggc ccacgctgcg gcaggcgtgt aataaggatc acacagggct
      301 gcatggcccg ccattctccg gcaggaagcc tggaggccat cgcaagggcg gtcgggagta
      361 ttccccggcc aaacgctttt tggacaaccc tcttgttgtc ttcccttgtg attggagcgt
      421 tccatgtcga actggttgag ttgccatttg ttgcttgtcg gtccccgacc tgagatcgaa
      481 cgttttgctg acgcggctgt ctacaaagtg ccggtcgctg atggcgagca ggagagcgtg
      541 ccgtttgcac tgcgagcgat ctgccctctg cctgacgtgc cggattgtgg tcccaccaca
      601 acacggcggg ttcgagaata ctgctggtcc acggattgcg atgtgaccgc ctgcggcgat
      661 gtcgagcgtg gcgacaccaa caccagtgat gaggccgaca gcacgactga gtatcgcttc
      721 tggtcggcct ccgcgccgcc catccgcgca attctgaccg cgagtaagcg gtttgatctt
      781 gaattccagt tgcgctggat tgaccgtttg ggccagacga cgggtggaga agtcacgatt
      841 cgggctggtc gtttcgtcga tgagcaggcc agcccgcaaa tcgagtatct gtttctggac
      901 gggagtgaac tgaatcggag ggaggctgag tgcgcggctg actattccgt cgagctttgc
      961 accgaccccg gcatctctag tgccgacaag ctcatggatg cgctgcggga acagactcac
     1021 ttccaccagc tttaggagaa ccctcgtgtc cgagaattgt gatttcagcg tcaccgtgaa
     1081 gggcgattac gctcaacttg aggacttgct tgaaacggcc ttggggccat ccgtcacgtt
     1141 ggtgatgggg aggttctatc ttgacccagg aagcgctggt gttcagattg acgaggcagc
     1201 aagcaagcag ttcgtttccc tccgcgatct acgcggggac gagcaaaaat ataacgccct
     1261 tcaacttgcc gactggccct gtttcaactc gtttgtcggg gattggcaca tcctccaaac
     1321 tccgcgcccg atgggcgaga ttgaccgtat caacgagttt ctcgaccgtt ccgccgccga
     1381 gcgccttgcg atgttcgacg agttcgtgaa actcgtcggc ctggagtacg agagcagcgc
     1441 cgccctggat gcgagccggg aggcactggc tcgttctgac tggccgcgta atcgctatcg
     1501 gggcctgctg gatcagccga tgccccgtct gctgctttgg ggagagtcgg attgggggcc
     1561 gcctctcata ttccaggcgc tggcaaagca ctggcctttg cttgttttcg tcgccggtgg
     1621 atcgaccgat tgcgaactct acgaggaata cgagtggtcc ggcgacagtg aacgctgcaa
     1681 ttacaggttg atcgtgaacc cccggactga tgaggtcgag tgggtcgtca aggacggcgt
     1741 ggtgatctca caagcgaagt ggcagcctgt ccgctagtgt cgcggtgtgc gaggactggg
     1801 gcttcccttg ccacagagaa tccgactggt acgctggcac gatgccaatt cttggagcgc
     1861 accaaagcat tgctggcgga tttcatcgcg cagttgagcg ggctgcggca gtcggctgtg
     1921 aaacagtcca aatcttcacg gcagcccctc accagtggcc caaaaacctg ccactgtctt
     1981 atcaaaaaaa ctcgaccaag aataacaacc agtggcgagc caaggcgatc acggaggcgg
     2041 aagcggaaca gtttgcggcg gcgctggccg agcggaagat cgcacatccg ctgtcgcatt
     2101 cgagctattt gatcaatctg gcgtcgcctg atgcggcgct gcggtcgaag tcggttgaat
     2161 cgatgatcgt cgaattgcag cgggcggcgc aactcggcat tccctacgtc gtcgttcatc
     2221 ccggtgcgca cacgaccgcc agcctggagg aaggactctc gctcgtcgcg aaatcactcg
     2281 acgagatcca cgccgcactt cccaagacac ctgctcagat cctgctggag atcaccgccg
     2341 gccaaggctc ctgcctggga catcggttcg agcacctggc cgagatcctc aagcaaacga
     2401 agaagcccga gcgcgtcggc atttgcttcg acacctgcca cgcctttgcc gccggctacg
     2461 acttgcgcgg ggcccagagc tacaaagcca tgtggtgcgc attcgacgac ctgctcggca
     2521 tcggccgcct gaaggccatc catctcaacg acagcaagcg cgagctgggc tcgcgcgtcg
     2581 atcgccacga gcatatcggc cgcggccaca tcggcgccga aggctttcgc ctgctgttgc
     2641 gcgaccgcca gcttcgcgag atcccgatgt acctggaaac gcccaaaggc gaccacgaag
     2701 gcgagacttg ggacgcgatc aacctccgca cgctgcgcga gctggccaaa gcctgatggc
     2761 cggctattcc ttctcccccc gccgcttgta atcgaccacg tcccag
//
LOCUS       DASUGB010000390         6051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_43495,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000390 DASUGB010000000
VERSION     DASUGB010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43495"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..2989)
                     /locus_tag="VFV87_07490"
     CDS             complement(410..2989)
                     /locus_tag="VFV87_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5103634.1"
                     /translation="MKSSISSLFVVVVAVSIPAAGEDHPDRLVQPKIPQGKLTAGEFS
                     DSRLFAGTRRQYSVYVPAQYRDDRPAKLMVFMDGSGYAKLDGAFRVPIVFDNLIHQRA
                     MPVTIAVFVNPGTIPATKPGASDRSNRSFEYDSLGDRYATFLVDEFLPAALKGLNVSK
                     DPQDRAVCGISSGGICAFTVAWERPDQFGKVMSHIGSFTNIRGGWAYPGLVRKTKDRP
                     KPIKVYLQDGKDDLNNLHGNWPLGNQDLAAALQFAGYHYKLVMTEGGHSGKWAGEDLP
                     NALRWLWSEDSEPTSIPVVETKPSWKPHPDAVAKDDVPHGTIEQMPAWESKIFSDTIR
                     DWSIYIPAQYKESQPAALMIFQDGERMRDINGRWRVPIVFDNLIARGEMPPTVAVFIN
                     PGHDKSRPLQNNRASNRGYEYDSLGDRYVRFLLEEIIPEVEKRYRISPDPEMRAVGGS
                     SSGAICSFTAAWERPDIFRKVYSSVGSFTNLRGGNVYPALIRKTEPKPIRVYMADTSG
                     DVDNAFGSWWWSNQRMASALKYMGYDVRFDSAEGYAHNADFGSSRFPEAMKWLWKEEP
                     HKPVLDTSGDLGGDLTLLNLLIPGESWEVAADKLGFADALCADADGNLYFSDMKAPAV
                     YRLNVNDGSRTEIVKEAVSGLKLSADGLLYGCQGAQNRLISINVNTGAVGVVASNVTP
                     NDLVVSADGFIFITETKAQQVTRIDIKKGTTSPVDTGISRPNGIALSNDGGTLAVSDH
                     GGEFAWMFRLNADGVLDAKLPTMPLRLPIDDAGEFKFNEPPPYITASRGDGMAVDKKG
                     RYYITSALGVQIFDPTGRPCGVLPKPDRDQPLTTCTLGGPHHSTLYIAHGSKVYRRKL
                     NVE"
     gene            3302..4420
                     /locus_tag="VFV87_07495"
     CDS             3302..4420
                     /locus_tag="VFV87_07495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX5103635.1"
                     /translation="MSRLLLSLALLLALGSLISAAEEKIPLGPPGATLDDVHQNPDDP
                     AILTKFASPHFREISMLSDEDPDAAEKKLAEFEAVLDSLKVGEKAQPLLTRLKASITA
                     YRENIASDRLTLADVEKELTENPDDIKAFRNWSNKALSEMYGIAYSRPEQAEAKLSKA
                     KEFLAKVSEAAQNDNTKERIASLSDPRRGSFSQIEQAIAKGREYAELIGKAAAPLTVE
                     TWVNGTPLTAGDLNGKVVLLDFWAVWCGPCIATFPHLREWQEKYGDKGLVIIGVTSYY
                     NYKWDDEAGYTARADGEVTPEEEQETLQKFAAYYHLKHRFAVQPEGDRTLSEHYGVSG
                     IPHVVVLDKTGKIRLIRVGSGKQNAEDIDKLLAELLKA"
     gene            4458..4682
                     /locus_tag="VFV87_07500"
     CDS             4458..4682
                     /locus_tag="VFV87_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103636.1"
                     /translation="MTKIREPSEAERREWRADFEAAARRTLEQRMRYAFIKTYKPVLD
                     DDDYRSFDTMEEYRQWCEANLPRWLGYHRV"
     gene            4679..4897
                     /locus_tag="VFV87_07505"
     CDS             4679..4897
                     /locus_tag="VFV87_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103637.1"
                     /translation="MNTGSRRRFTRYSHVTDQPESSLQERIEELAAKSSEGELTDAER
                     AEYVGYVQANKFVAILQAQARRRLAGSR"
     gene            4897..5037
                     /locus_tag="VFV87_07510"
     CDS             4897..5037
                     /locus_tag="VFV87_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103638.1"
                     /translation="MDAATRRFIRERAGNRCEYCQLSQEHSPLFVLQIELRSLVVRPP
                     SS"
     gene            5070..6029
                     /locus_tag="VFV87_07515"
     CDS             5070..6029
                     /locus_tag="VFV87_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5103639.1"
                     /translation="MPHAIECRQLVKRYDGKPPVEAVRGLDLVVNEGECFGLLGPNGA
                     GKTTTLEILEGLLDATSGDVQVLGLRWGTHDAEIRERIGISLQETRLADKLSVRETVT
                     MFRSFYRAGIEPDEAIRRVSLEEKATAWVVKLSGGQKQRLAVACALVGDPQLLFLDEP
                     TTGLDPASRRQLWEVIHEFRKTGRTVMLTTHYMDEAERLCDRVAVIDHGQVIALGTPR
                     ELIAQLGGENVIEFTLADGSPAADEGELARLPAVRSVKREAAAYHLAVQQPHVALPSL
                     VAHLQASGRTLANLTTRHATLEDVFVSLTGRHLQEERPADAAA"
BASE COUNT         1221 a         1799 c         1858 g         1173 t
ORIGIN      
        1 agcgtgcgcc gccgcaagtc gttgcggcag gctgacttcg caaacaggat tttttaaggc
       61 atggctccgg tgtccaatgg gtttatgaaa gcggcccctg gaagcaatcg ccaccagggg
      121 cctggatgca ccattggcgc accgtcggcc cggatatccc ttgtcaggtt gcttcccagc
      181 agagcctgac tccgtttctc cgggcaggcc cagggtactc gaaacgaccg ttctgttcca
      241 actcccatta gacaccggag ttatgcctcc aaaatccaag gaacccggcg gggaacccct
      301 cgcgcacgct tcagtcggaa gagccgattt tgcttcccaa gcctcttata ccgaacggtt
      361 tatcacacgt ggacttcggt tggcaaaccc actgggaagg tgacaaactt cattcaacat
      421 tcagcttccg tcgatacacc ttcgatccgt gggcgatgta gagtgtgctg tgatgcgggc
      481 ctcccaacgt acacgtggta agtggctgat cgcgatccgg cttgggcaat acaccgcagg
      541 ggcgtcccgt ggggtcaaaa atctgtactc ccagagcgct ggtaatgtaa tagcgtccct
      601 tcttatcgac cgccattccg tctcctcgcg aagctgtgat gtatggcggc ggctcgttga
      661 acttaaactc ccccgcatca tcaattggga gccgcagtgg catggtgggc agcttcgcat
      721 ctaacacgcc gtccgcgttg agtcggaaca tccaagcaaa ttctccgcca tgatcggaga
      781 ccgctaacgt gcctccgtcg ttggacagag caatgccatt gggcctcgag atgcccgtat
      841 cgaccgggct cgtcgtcccc ttcttgatgt cgatgcgcgt cacttgctgg gccttggttt
      901 cggtgatgaa gatgaagcca tcggcgctca caacgagatc gtttggcgta acattgctgg
      961 cgacgactcc gacggcgccc gtgtttacat tgatcgagat caggcgattc tgcgcacctt
     1021 ggcagccgta cagaagaccg tcggcactga gtttcagacc actcaccgcc tccttaacaa
     1081 tttcggtccg ggatccgtca ttgacattca ggcgatagac ggccggggcc ttcatgtccg
     1141 agaaataaag gttgccgtcc gcatcggcgc aaagtgcatc ggcaaatccg agcttatccg
     1201 cggcgacttc ccacgattcg cctgggatca acaggttcag cagcgtcagg tcgcctccca
     1261 gatccccgct ggtgtcgagc acaggcttgt gcggttcctc tttccagagc cacttcatgg
     1321 cctccgggaa gcgggagctg ccgaaatcgg cattgtgggc ataaccctct gcggaatcga
     1381 accggacatc gtagcccatg tacttcagcg ccgacgccat ccgttgattc gaccaccacc
     1441 agcttccaaa ggcgttatcg acgtcgccgc tcgtgtcggc catgtacacg cgaatgggct
     1501 ttggctcggt cttgcggatc agcgccggat agacgttgcc gccacgcaga ttggtgaagc
     1561 tgcccacgct cgaatacact ttgcggaaaa tgtccggacg ttcccaggcg gcggtaaatg
     1621 agcaaattgc tccggaactg gatccgccaa cggcccgcat ttcaggatca ggagagatgc
     1681 gatagcgttt ttccacctcg gggataatct cttccagcag aaagcggacg tatcgatcgc
     1741 cgaggctgtc atattcgtag ccccgattgg aagcccggtt attctgcagc ggcctcgatt
     1801 tgtcgtggcc gggattgatg aacacggcga ccgtcggggg catctccccg cgggcgatca
     1861 gattgtcgaa cacgatcggg acccgccagc ggccgttgat gtcccgcatc cgctcaccgt
     1921 cttgaaagat catcaaggcg gcgggctggc tttccttgta ttgcgcaggg atatagatcg
     1981 accagtcgcg gatcgtgtca gaaaatatct tcgattccca ggcgggcatc tgctcgatcg
     2041 tgccatgagg gacatcgtcc tttgccacgg catcgggatg cggcttccag gaaggtttgg
     2101 tttccacgac gggaatgctg gttggttcgg agtcttccga ccagagccag cggagggcgt
     2161 tcggcagatc ctcgccagcc catttgccgc tgtggccccc ttcggtcatg acgagcttgt
     2221 agtggtaccc agcaaattgg agggctgccg cgagatcctg atttcccagt ggccaattgc
     2281 cgtgcagatt gttgaggtca tctttgccgt cctgcaggta aaccttgatc ggcttgggac
     2341 ggtccttcgt tttgcgcacc agtcccgggt aggcccagcc gccgcgaatg ttcgtgaaac
     2401 tgccgatatg gctcatcacc ttgccgaatt gatcgggcct ctcccaggcg accgtgaagg
     2461 cacagatgcc gcccgatgaa atcccgcaca ccgcgcggtc ctggggatcc ttcgatacat
     2521 tcagaccttt cagggcagcg ggaagaaatt cgtccacgag aaaggtggca tagcggtctc
     2581 ccagagaatc gtattcaaaa gatcgattgc tgcgatcgct ggccccaggc ttggtggcgg
     2641 ggatcgtccc gggattcacg aagacggcga tggtcacggg catcgcccgc tgatggatca
     2701 ggttgtcaaa gacaatagga acccgaaacg ctccgtcgag cttggcatag cccgacccgt
     2761 ccatgaacac catcaatttc gcaggccgat catcccggta ctgggctggg acgtacacgc
     2821 tgtactgccg tcgcgtgccg gcaaagagac ggctatcgga gaattcgccc gccgtgagct
     2881 tgccctgggg aattttcggc tgaacgagac gatcggggtg gtcttcgccc gcggctggaa
     2941 tcgaaacagc gacgacaacg acaaacaagc tgctgatcga cgacttcatg gtatgcctgc
     3001 gggatcaaag gggcggggcg tcattaaacc gttacgatcg cggcttgccg agaccgggaa
     3061 aatgccacga tgtcatgcac gacggaacgg atcatcgctt ctcgtctgcg gccgggcaag
     3121 tagctccagt gggactgaac cggcgaaatc ccccctttgc cgtctgactc gagagcgggg
     3181 gcttatgcag gcgatcctga cctgtcgtag gctctcgctg gttcaatagc cgactcttgc
     3241 agccgtctgg ccgcgggcat ctaatgggat cgtcgtgccg tcctccaata aggaattccc
     3301 catgtcccgc ctgttgcttt cgctcgcatt attacttgcc ctgggaagtc ttatttccgc
     3361 tgccgaggag aagatcccgc tgggaccgcc gggggcgacg ctggacgacg tgcatcagaa
     3421 tcccgacgac ccggcgatcc tcaccaagtt cgccagcccg catttccgcg agatcagcat
     3481 gctgtcggac gaggatcccg acgccgcgga gaagaaactc gccgagtttg aagccgtgct
     3541 cgattcgctg aaagtcggcg agaaagcaca gccgctgctg acgcggctca aggccagcat
     3601 caccgcctat cgcgagaata tcgccagcga tcggctgacg ctggcggatg tggagaagga
     3661 actgaccgag aacccggacg atattaaggc ctttcgcaat tggtcgaaca aggctcttag
     3721 cgagatgtac ggcatcgcct attcccgtcc cgagcaggcc gaggcaaagc tgtccaaagc
     3781 caaggaattc ctggcgaaag tttcggaagc cgcgcagaac gacaacacca aggaacgcat
     3841 tgccagcctt tccgaccccc gccgcggcag tttttcgcag atcgagcagg cgatcgcgaa
     3901 gggtcgcgaa tacgccgagt tgatcggcaa agctgccgcg ccgctgacgg ttgaaacatg
     3961 ggtcaacggc acgccgctga cggcgggcga cttgaacggc aaggtcgtgc tgctcgattt
     4021 ctgggccgtc tggtgcggac cgtgcatcgc aactttcccg cacctccgcg agtggcaaga
     4081 aaagtatggc gacaagggac tcgtgatcat cggtgtaacc agctactaca actacaagtg
     4141 ggacgacgaa gccggctaca cggcccgcgc cgacggcgaa gtgacgcccg aagaggaaca
     4201 ggaaacattg cagaagtttg ccgcctatta ccacctcaag caccgcttcg ccgtccagcc
     4261 cgaaggtgac cgcacgctct cggagcatta cggcgtcagc ggcattccgc acgtggtcgt
     4321 cctcgacaag acaggcaaaa tccgcctgat ccgcgtcggc tccggcaagc agaatgcgga
     4381 agacatcgac aagctgctgg cggagttgct caaagcctga gcgataaagg tctggcgtct
     4441 taccggagtc catgcccgtg accaagatcc gcgaaccctc ggaagccgag cgccgcgagt
     4501 ggcgggcgga ttttgaagcg gcagcgcggc gcacgctgga gcagcggatg agatacgcgt
     4561 ttatcaagac ctacaaaccg gtgctcgacg acgacgacta tcggtcgttt gatacgatgg
     4621 aagagtatcg ccagtggtgc gaagcaaact tgccccgttg gttgggctat caccgtgttt
     4681 gaataccggc tcgcgcagga ggttcacgag gtattctcac gtcaccgacc agcccgaatc
     4741 gtcgctgcag gagcgaatcg aggagcttgc cgcgaagagc agcgagggcg aattgaccga
     4801 cgctgaacgc gccgagtacg tcggctacgt gcaggccaat aagttcgtcg ccatccttca
     4861 ggcccaagcc cgtcgacgcc tggcaggaag ccgctaatgg atgccgcaac caggcggttc
     4921 atccgggagc gggcgggaaa ccgctgcgag tactgccagc tatcgcagga gcactctcca
     4981 ctgttcgttc tgcagatcga gctgcggtcg ctggtcgtgc gtccgccgtc ttcgtaggat
     5041 tgcaacagcg ttgccaaccg actggctata tgccccacgc cattgaatgc cggcagcttg
     5101 tcaaacgtta cgacggcaag ccgccggtgg aggcggtgcg tggcttggat ttggtcgtaa
     5161 acgaaggcga gtgctttggc ttgctcggcc ccaacggagc gggcaagacg acgacgctgg
     5221 agattctgga ggggttgctc gacgccacca gcggcgacgt gcaggtgctg gggctccgct
     5281 ggggaacgca cgacgcggag attcgcgagc ggatcggcat ttcgctgcag gagacgcggc
     5341 tggcggacaa gctctccgtg cgcgaaaccg tgacgatgtt ccgcagcttc taccgcgcgg
     5401 gcatcgagcc ggacgaggcg atccgccgcg tctcgctcga agaaaaggcc actgcctggg
     5461 tagtcaaact ctcgggcggg cagaagcagc ggctggcggt ggcctgtgcg ctcgtcggcg
     5521 atccgcagct tttgtttctc gacgagccga cgaccggact cgacccggcg tcgcggcggc
     5581 aactgtggga ggtgattcat gaattccgca agaccggccg gacagtgatg cttacgacgc
     5641 attacatgga cgaagcagag cgattgtgcg accgcgtcgc ggtgatcgat cacggacagg
     5701 tgatcgcttt gggcacgccg cgggagttaa tcgcgcagct tggcggcgag aatgtgatcg
     5761 agttcacgct ggccgacggt tcgccggcgg cggacgaggg tgaattggcc cgcttgccgg
     5821 cggtacgctc ggtcaagcgc gaggccgcgg cgtatcacct ggcggtacag cagccgcatg
     5881 tggcgttgcc gtccctcgtc gcccatttgc aggcaagtgg gcggacgctc gcaaacctga
     5941 ccacgcggca cgcgaccctg gaggatgtgt tcgtctcgct cacggggcgg cacttgcagg
     6001 aggagaggcc cgccgatgcc gccgcgtaaa tactggcccc tcggccagct c
//
LOCUS       DASUGB010000391         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1849362, whole genome shotgun sequence.
ACCESSION   DASUGB010000391 DASUGB010000000
VERSION     DASUGB010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849362"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFV87_07520"
     CDS             <1..530
                     /locus_tag="VFV87_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HEX5103640.1"
                     /translation="VMSFGAPEIAAGIEQIFREKLDELARAIHERYVAQRLADGAKPG
                     SDPALSSWSLLDAQFKSSNRQQADHIDVKLRAASCRRLAEGEETPAGSKPFEGFTKDE
                     VELLAHMEHARWCADRYLGGWRVGEQADKLNKISPYLIPYDQLTEEIKQYDREAVLQI
                     PELVQLASQRIVRVA"
     gene            complement(733..3687)
                     /locus_tag="VFV87_07525"
     CDS             complement(733..3687)
                     /locus_tag="VFV87_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5103641.1"
                     /translation="MNRIEEAAVVSRELKKLSGDEGPLSPEESLAKCTTPDDLVAEIS
                     LAEPHIGQPLSLKWDSRGRLWVVNYLQYPNPAGLKMVSRDKFLRSVYDQVPPPPPNHF
                     PGADKITIHEDTDGDGQFDKHSTFLEGLSLMTSFEFGKGGIWVLQPPYLLFYPDANGD
                     DVPDGDPVVHLEGFGIEDSHSVTSHLRFGPDGWLYASQGSTVTGAVKRPGDKTAVHSM
                     GQLIWRYHPEQKKYEIFAEGGGNAFGVEIDSQGRVYSGHNGPNTRGFHYVQGGYLQKG
                     FGKHGELSNPFTFGYFEAMAHHQVLRFTHTFVIYNGGALPEPYNGRLFGVAPLLSHVV
                     MSDFMPDRSSFKTKDVGHLLESSDPWFRPVEIQVGPDGAVYVADMYEQRIDHASHYQG
                     RISKDTGRIYRIRTRGERPKLAPFDLKKETSEQLIARLSEKNKWFRQAALREIRLRQD
                     KSLAPLLLEKLSAARGQEAVEYLWAANAVGAIGDEQTLALLDHADPYVRVWTVRLACD
                     DGEVTSEIAEKLAELAYREPHVEVRSQLACSARRLPAGQSLAIVKNLAHRSEDVDDIH
                     IPLLLWWAIEHHADASRDEVLNLFADKAFWNEPLVNKHLLERTMRRYASTGQRKDLLT
                     GAKLLELAPTKEHAGKLMAGFEAAYEGRTIANLPAELAAAMAKAGAASPTVRLRQGEP
                     AAVTEALATIGDESADASKRQQLIVLFGTLNQPTCVPVLLKVAKESRNDALRSAALGS
                     LQSYGSPEIGSSVLALYKDMSDQVREVAQSLLSSRKAWAEGLLAAVDAGQIDKQTISE
                     PTVRKLLLHESPRIAELCQKHWGALSGPDPDALRAEIEKLIGMIGTGSGNPYQGKALY
                     GQNCGKCHVLFTQGGKIGPELTNYKRDDLRGMLLNVVNPSAEIREGFENYIVRTLDGR
                     TLTGFIADQDANVVVIKGADGQSITLARDEIEDLRASRTSLMPEGQLKTLSDQQIRDL
                     FAYLRATQPLPE"
BASE COUNT          638 a         1223 c         1131 g          716 t
ORIGIN      
        1 atgtcatgtc gttcggcgcg ccggagattg ccgccgggat cgaacagatc tttcgggaga
       61 agctcgacga gctggcccgg gcgatccacg agcgctatgt cgcccaacga cttgcggacg
      121 gcgctaagcc cggcagcgat cctgcgctct cctcgtggtc gctcctcgac gcccagttca
      181 agagttccaa ccgccagcag gccgaccaca tcgacgttaa gctccgcgcc gccagctgcc
      241 gccgtctggc cgaaggcgaa gagacgccgg ccgggtcaaa gccattcgag ggcttcacca
      301 aggacgaagt cgagctgctc gcccacatgg agcacgcccg ctggtgcgcc gaccgctatc
      361 tcggcggctg gcgcgttggc gaacaggccg acaagctgaa caagatcagc ccctatctca
      421 ttccctacga ccagctcacc gaagagatca agcaatacga ccgcgaagct gtcctgcaaa
      481 tccccgagct cgtccaactc gccagccagc gaatcgtgcg ggtcgcctga caggcgaagg
      541 actggagcca cggatgaaac actgatcaaa cacgcatggc ctgcagctcc aaagacaaag
      601 tcacgaagaa caccaagggc gaggatcgcc cgccttcgtg ctcttcgtgc cttggtggtt
      661 ggctcctcgt gcagttcggc tccgacgtca gtgttccatt cgtattccgt ccgtggctaa
      721 gccctctccg atctactccg gtaagggttg cgtcgctcgc aaataggcaa acaggtcgcg
      781 aatttgctgg tcgctgagcg tcttaagctg cccttcgggc atgaggctgg tgcggctggc
      841 gcggaggtct tcgatttcat cgcgggccag cgtgatgctc tgcccgtcgg ctcctttaat
      901 cacgacgacg ttcgcgtcct ggtcggcgat gaagccggtg agcgtgcggc catcgagcgt
      961 gcggacgatg tagttctcga agccttcgcg gatctccgcg ctcggattga cgacgttcag
     1021 cagcatgccg cggaggtcgt cgcgcttgta attggtcagc tccgggccga tcttgccccc
     1081 ttgcgtgaag agcacgtggc acttgccgca gttttggcca tagagggcct tcccctgata
     1141 tggattaccc gagccggtgc cgatcatgcc gatcagcttc tcgatctccg cccgcaaggc
     1201 gtcgggatcc ggtcccgaca gcgcgcccca gtgcttctga cacagctcgg cgattcgcgg
     1261 cgactcgtgc aacagcagct tacggaccgt cggctcgctg atcgtttgct tatcgatctg
     1321 gcccgcatcg accgccgcga gcaagccttc cgcccaggct ttgcggctgg aaaggagcga
     1381 ctgggcgacc tcgcggacct ggtcggacat gtccttatag agcgccagca cgctgctgcc
     1441 gatttcggga ctgccatacg actgcagcga gccgagcgcc gcgctgcgga gagcgtcgtt
     1501 ccgtgattct ttcgcgacct tgagcagcac cggcacgcac gtcggctggt tgagcgtgcc
     1561 gaacaggacg atgagttgct ggcgcttgct ggcatcggcc gattcgtcgc cgattgtggc
     1621 cagggcctcc gttaccgcag ccggctcgcc ttgccgcagg cggaccgtag gcgaagccgc
     1681 gccagccttc gccattgccg ccgccagctc cgccggcagg ttggcgatcg ttcgcccctc
     1741 gtaagccgct tcgaagccgg ccatcagctt gccggcgtgc tccttggtcg gcgcgagctc
     1801 cagcagcttc gcgccggtga gcaggtcctt ccgctggccg gtcgaggcat accgccgcat
     1861 cgtccgctcg agcaagtgct tgttcaccag cggctcgttc cagaaggcct tgtcggcgaa
     1921 gaggttgagc acttcgtcgc ggctggcatc ggcatgatgt tcgatggccc accacagcaa
     1981 gagcggaatg tgaatatcgt caacatcctc gctccgatga gccaggttct tgacgatcgc
     2041 cagcgactgc cccgccggta gccggcgggc cgagcaagcg agttggctgc ggacttcgac
     2101 gtgcggctcg cgataggcca gttccgccag tttttcggcg atttcgctcg tcacttcgcc
     2161 gtcgtcgcac gccagccgaa ccgtccacac gcggacatac ggatcggcgt gatcgagcag
     2221 cgccagcgtc tgctcgtcgc cgatcgcgcc cacggcgttc gcggcccaca gatattccac
     2281 cgcctcctgc cccctcgctg ccgacagctt ttcgagtagc agcggggcga gcgacttgtc
     2341 ctgccgcaag cgaatctcgc gcaaggccgc ctgccggaac cacttgttct tctccgacag
     2401 tcgggcgatg agctgctcgc tcgtttcctt cttcagatcg aacggcgcga gcttcggccg
     2461 ctcgcccctg gtgcgaatgc gatagatgcg cccggtgtct ttgctgatcc ggccttgata
     2521 gtggctggcg tggtcaatcc gctgttcgta catgtcggcg acgtaaaccg cgccgtcggg
     2581 gccgacctga atctcgaccg gccggaacca cgggtcgctc gattcgagca ggtggccgac
     2641 gtccttcgtc ttgaacgacg agcggtccgg cataaagtcg ctcatcacca cgtgactcag
     2701 caacggcgcg acgccgaaca gccggccgtt gtacggctcg ggcagtgctc cgccgttgta
     2761 aatgacgaat gtgtgcgtaa accgcagcac ctggtggtga gccatcgctt cgaagtagcc
     2821 gaacgtgaac ggattcgaca actcgccgtg tttgccgaag cccttttgca gatacccgcc
     2881 ctgcacgtaa tgaaagccgc gggtattggg gccgttgtgc ccgctataaa cgcggccttg
     2941 cgaatcgatc tcgacgccga acgcgttgcc gcccccttcg gcgaagattt cgtacttttt
     3001 ctgctcgggg tgatagcgcc agatgagctg ccccatcgaa tgcacggccg tcttgtcgcc
     3061 cggccgcttc accgccccgg tgaccgtgct cccctgcgaa gcatacagcc agccgtctgg
     3121 cccaaagcgg aggtggctcg tcacgctatg cgaatcctcg ataccaaaac cctccagatg
     3181 cacaaccggg tcgccgtcgg gcacgtcgtc accgttggcg tcgggataaa acaacaaata
     3241 cggcggctgc agaacccaga ttccgccctt gccgaactcg aacgacgtca tcaggctcag
     3301 cccttcgaga aacgtgctgt gcttgtcgaa ctggccgtcg ccgtcggtgt cttcgtggat
     3361 cgtgatcttg tccgcgcctg ggaaatggtt cggcggcggc ggcggaacct ggtcatagac
     3421 gctccgcagg aacttgtcgc ggctgaccat cttcaggccc gcgggattcg gatactgcaa
     3481 ataattcacc acccacagcc ggccgcggga atcccacttc agcgacagcg gctggccgat
     3541 atgaggttcg gccagggaga tttcggcgac caggtcgtcc ggcgtcgtgc atttggccag
     3601 cgactcttcg ggcgagagcg gaccctcatc cccggagagc tttttcagct cgcggctgac
     3661 aaccgccgcc tcttcgatcc tgttcaatac gggcgtcttg atcgcggc
//
LOCUS       DASUGB010000392         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_598445, whole genome shotgun sequence.
ACCESSION   DASUGB010000392 DASUGB010000000
VERSION     DASUGB010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598445"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFV87_07530"
     CDS             complement(<1..479)
                     /locus_tag="VFV87_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103642.1"
                     /translation="MPLFLPPLVFGLDPLLDRDPVGAEGLRPFVVLPLRRVQHPRFRD
                     QLLALGPLLLPLRFFPATLGVPPLLLALELRSGLACILVADLGGGLPLWLRTTALFRP
                     LASSFKSGRSACSAKRPFDPAGRLSTTPGFDMVAPITSGSSVSLAASWWKRLLPAPQA
                     "
     gene            765..905
                     /locus_tag="VFV87_07535"
     CDS             765..905
                     /locus_tag="VFV87_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103643.1"
                     /translation="MAPASFNTEMAQPRSVRTAGRFFFVISIGITPAKTNPSDRRHVP
                     GG"
     gene            complement(945..2072)
                     /locus_tag="VFV87_07540"
     CDS             complement(945..2072)
                     /locus_tag="VFV87_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5103644.1"
                     /translation="MNRSEDKPGRGAAYSGWDIAATLGLLAVAFLVERSDPAKAPNLA
                     QQTGEKRDAPSAGLASAEPGRGRHAASPSQIPAKGWKDVLWRVYGNVGDHRILALAAG
                     MTYYSILAIFPALAALVAIYSLFSDPATIAKHLDELAGVIPGGAVDIAGEQLTRVASK
                     GNNTLGFTFAIGLAISLWSANAAMKSLFDTLNIVYGEQEKRSFLKLNAMSLGFTIAGI
                     AFILSALAAVVVVPLVLQYLWLSNAADLLIRIARWPAMFVTLAVGLARIYRFGPSREA
                     PRWKWITWGSAAATILWLAASALFSWYAANFGTFNETYGSLGAAIGFMTWLWISAIAI
                     LLGAELNAEIEHQTVRDTTTGKPKPLGARGAKMADTVGAAR"
BASE COUNT          428 a          667 c          712 g          359 t
ORIGIN      
        1 gcctggggcg ccggcagcaa ccttttccac caggatgccg ccaaagagac cgacgatccc
       61 gaggtgatcg gcgcaaccat gtcgaaaccc ggagtggtgc tcaagcggcc ggcaggatcg
      121 aacggccgct ttgccgaaca cgccgacctg cctgatttga aggacgaagc gagcgggcgg
      181 aaaagagcag tcgtcctgag ccaaaggggc aggccacccc caagatcagc gacaaggatg
      241 cacgcaaggc cgctgcggag ttcgagcgcg agcaggagcg gcgggacgcc gagcgtcgca
      301 gggaagaagc gcagagggag aaggagcggc ccaagcgcga gaagctgatc gcgaaagcgc
      361 ggatgctgga cgcggcgcag cgggagcacg acgaacggtc ggaggccctc cgcgccgacc
      421 gggtcgcgat ccagaagtgg gtccaggccg aagacgagcg gtgggagaaa gagaggcaaa
      481 ggctcacggc cgcgctgcgc cgcgctcggg actaaagcat ccgcgtttgg cgccgacacg
      541 cgtctcggca aaagcgtgct gtttggtaga tgacggacct agctttcgtc cggcggacaa
      601 cccgggcagg tgtcgccgat cgaatcgagg acgtcgcgga cctccgccac ggctccttcc
      661 gcggagatgc cggtcggcgg gcttgccggg cgagatcgat ggcgcgctcg cgagcgtgcg
      721 ggtcggcccg gtcctgcgcc cagccgtgct cctcgcattc ccggatggcg ccagcttcgt
      781 tcaacaccga gatggcccag ccgcgcagcg tgcggacagc cggtcgcttt ttcttcgtca
      841 tcagcatcgg gatcactcct gccaagacga atccttccga ccgccggcac gttcccggcg
      901 gttaacacgg gtcagggatt ccgaatgccg aggctccggc ttgtctaccg agccgcgccc
      961 actgtatcag ccattttagc gccccgcgcg ccgagtggtt tgggcttgcc ggtcgtggtg
     1021 tcgcgcacgg tctggtgctc gatctccgcg ttgagttcgg ctccgagcaa tatcgcaatc
     1081 gccgagatcc agagccaggt catgaagccg atggctgccc cgagtgagcc ataggtttca
     1141 ttgaacgtcc cgaaattggc tgcataccag gaaaagagcg cagaggcggc gagccacagg
     1201 attgtggccg cggcgctgcc ccacgtgatc catttccagc gcggagcctc acggctcgga
     1261 ccgaatcggt aaatgcgggc taggcccact gcgagagtaa caaacatggc cggccagcgt
     1321 gcgatccgga tcagaagatc ggccgcgttc gatagccaaa gatactgaag gaccaacggc
     1381 acgacgacca cggctgccag cgccgacaga atgaaggcaa tgccggcgat tgtgaagccc
     1441 agcgacatcg cgttcagttt caggaagctg cgcttctcct gctcgccgta gacaatgttc
     1501 aacgtatcga acagggattt catggcagcg ttcgcgctcc agagagatat cgccagcccg
     1561 atggcgaacg taaaccctag cgtgttgttt cctttcgaag ccacgcgcgt gagctgctcc
     1621 ccggcgatgt ccaccgcacc gcctggtatg acgcctgcca gttcgtcaag atgcttcgct
     1681 atggtggcag gatcggaaaa caggctatag atcgcgacca aggccgccag cgcaggaaag
     1741 attgcaagga tgctgtaata ggtcatgccg gccgccaggg cgagaatgcg gtgatcgcct
     1801 acattgccgt agacacgcca caggacgtcc ttccatccct ttgccgggat ttgggagggc
     1861 gacgccgcgt gacgacctcg ccctggctcc gctgatgcca agccggcgga cggcgcgtca
     1921 cgcttttcac ctgtttgctg tgcaagattg ggagccttcg ccgggtcact gcgttcgacc
     1981 aggaaggcga cggccagcaa cccgagcgtc gcagcgatgt cccatccgct gtacgcggcg
     2041 cctcgacccg gtttgtcttc tgacctgttc attggcgtct ccacggaggt aatcgtcctc
     2101 attggtcggg agttcctcat ggaaggagca ccggtgcgga atttgcgtgg cgttgagagt
     2161 accaac
//
LOCUS       DASUGB010000393         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1017225, whole genome shotgun sequence.
ACCESSION   DASUGB010000393 DASUGB010000000
VERSION     DASUGB010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017225"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFV87_07545"
     CDS             complement(<1..480)
                     /locus_tag="VFV87_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103645.1"
                     /translation="MATHVLRGGALLALAILCAAAAGQNDDGGFIGVLPATTVQLPTF
                     GIAIDAEGTLDARTFKDFDGKLLRERLVAAKAAKPGELWAAAKLRKVSLMRLEAALAA
                     EIDAGRGPDDVLRNLAGLTRVQYVFCLPAADGKPGDVILAGPAEPFALDPAGRAVGLM
                     "
     gene            complement(635..919)
                     /locus_tag="VFV87_07550"
     CDS             complement(635..919)
                     /locus_tag="VFV87_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103646.1"
                     /translation="MVVASGALPLFTRSAGADPMQASFEETLKFGLKCRRPLEFAFVE
                     LVVLKVNQGQLPKDLVLSQFNWAREQNEKIPFPYFQFGMKKRAAALGVDL"
     gene            1315..>2446
                     /locus_tag="VFV87_07555"
     CDS             1315..>2446
                     /locus_tag="VFV87_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103647.1"
                     /translation="MLCIPGQPGKDLCDSHLGVTRRDILRVGGSAVLGMSLGGMLQLK
                     DTQAAPPVGNAFSGGGPGWNKAKSVILVYLQGGPSHLDLWDPKENVPDNVKSVFKKVP
                     SKVPGMEMTENLPKLAEHTDKFTFIRSMSYTPVGLFNHTAAIYQIHTGYTADKVSPSG
                     QLEPPTPKDFPNFGCNIVRLKPPEVPMLPFVMLPRPLQESNVVNKSGTAGFLGRAYDP
                     YYLFPPGDDMDMAKMDRIRVDDLTLRAEVSEARLDRRARLRDLINQGMPEVDKAVAKY
                     DLDKYYDSALSLIMSGRAREAFSLNKESDAMRERYGRNTFGQSCLLARRLVEAGTRVV
                     EVIWPKVANSDNHSFDVHTGLSDRMKKQSGPMLDAGLSGLISD"
BASE COUNT          478 a          817 c          721 g          430 t
ORIGIN      
        1 catcaggccg accgcgcggc cggctggatc aagggcaaag ggttcggcgg gaccggcgag
       61 gatgacgtcg cccggcttgc cgtcggcggc aggcaggcag aagacgtatt gcacccgcgt
      121 caacccagcc agattccgca gcacgtcgtc agggccgcgc ccggcgtcga tctccgccgc
      181 cagcgccgcc tccagccgca tgagcgagac cttgcggagc ttggccgccg cccacagctc
      241 gcccggcttg gcggctttcg ccgccacgag ccgctcgcgc aagagcttgc cgtcgaagtc
      301 cttgaacgtc cgggcgtcga gcgtgccttc cgcatcgatg gcgatgccga atgtcggaag
      361 ctgcactgtg gtcgccggca gaacgccgat aaagccgccg tcgtcgttct ggcccgcggc
      421 tgccgcacaa agaattgcga gcgccaacag tgcgccgcca cgaagaacat gcgtggccat
      481 gaaaatcgcc ttgttcgctg cccctggggt catgctaccg cggatgtacc gcgcgggcaa
      541 cgaaaagtgg ggcagacatt cttgtctgcc acgttggccg gcacaaagct gtctagcggc
      601 agacaagaat gcctgcgcca agggccgtcg agacctacag atcgaccccc agcgccgcgg
      661 cgcgcttctt cattccgaac tgaaagtacg ggaaggggat cttctcgttc tgctcgcgag
      721 cccaattgaa ctggctcagc acgagatctt tgggcagctg gccctgattg accttgagca
      781 caaccagttc gacgaacgcg aactccagcg gcctccggca cttgagaccg aacttcaacg
      841 tctcctcgaa actcgcttgc atcgggtcgg cgccggccga gcgtgtaaac agcggcagcg
      901 cgccgctggc gacgaccacc cgaatccagg ttcgacgatt cataccgcgg gatgtaccaa
      961 aacggcccgc ccgtttgcaa catcaaccaa cccggccaac cggcgtccat tttattcaaa
     1021 tcccagccgc cggcacgggt gtcctatctc caaacccgga ttgactttag atcggcgaca
     1081 cctatcgccg atactgccct gggaaaaccg atcaaaagat tgacattcga ctccgcgggc
     1141 cgctatattg cggaaagttg aaaaggttgg gaaagtccgc cgactgccgt tccgtctggg
     1201 acatggcaga ggtggcgttt ccagccttcc cgcctgcgct gttggctcca ttgccgcagc
     1261 caagaattcg cttccctcgc caattgcccg cctctgagga gctgcctgat gtcgatgctc
     1321 tgcattcctg gacaacccgg caaggacctg tgtgatagcc atcttggcgt gacccgtcgc
     1381 gatattctcc gcgtcggcgg ctcggcggtc cttggcatgt cgttgggcgg tatgctccag
     1441 ctcaaggaca cccaggcagc cccacccgtc ggcaacgcct ttagcggcgg cggccccggc
     1501 tggaacaagg ccaagagcgt catcctggtt tatctgcagg gcggccccag ccacctcgac
     1561 ctctgggatc ccaaggaaaa cgtgccggat aacgtcaaga gtgtgttcaa gaaagtcccc
     1621 agcaaggtcc ccggcatgga gatgaccgag aacctgccga agcttgccga gcacaccgac
     1681 aagttcacgt tcatccgctc gatgagctac accccggtcg gccttttcaa ccacacggct
     1741 gccatctatc agatccacac gggctacacc gcagataagg tcagcccctc cggccagctc
     1801 gaaccgccga ctcccaagga tttcccgaac ttcggctgca acatcgtccg cctcaagccg
     1861 ccggaagtcc cgatgctgcc gttcgtcatg ctcccgcgcc ccttgcagga gagcaacgtc
     1921 gtcaacaagt cgggcacggc cggctttctt gggcgggcat acgatccgta ctacctgttc
     1981 ccgccgggcg acgacatgga catggccaag atggatcgca tccgcgtcga tgacctgacg
     2041 ctgcgggccg aagtcagcga ggcccggctc gatcgccgcg cccgcctccg cgacttgatc
     2101 aatcagggca tgcctgaagt cgataaggcg gtggccaagt acgacctcga taagtattac
     2161 gactcggctc tgagcctgat catgtcgggc cgcgctcgcg aggccttctc gctcaataaa
     2221 gagagcgatg cgatgcgcga gcgttacggg cgcaacacgt tcggccagtc gtgcttgctg
     2281 gcccgccgcc tggtcgaagc ggggacgcgg gtcgttgaag tcatctggcc gaaggttgcc
     2341 aatagcgata accatagctt cgacgtgcac accggcctct ccgaccgcat gaaaaagcag
     2401 tcggggccaa tgctcgacgc cggcctttcg ggcttgatct cggacc
//
LOCUS       DASUGB010000394         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1573333, whole genome shotgun sequence.
ACCESSION   DASUGB010000394 DASUGB010000000
VERSION     DASUGB010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573333"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFV87_07560"
     CDS             <1..296
                     /locus_tag="VFV87_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103648.1"
                     /translation="WRKERNSATWQKNAKSFNGEALQRKLRGEKVRVARNIAPARTLF
                     TCTASAIRGVFCGANRSPAQSLHPAARLDVPMMGVGEQTKWGFGREGQVIDGG"
     gene            325..1599
                     /locus_tag="VFV87_07565"
     CDS             325..1599
                     /locus_tag="VFV87_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5103649.1"
                     /translation="MSDIDSQSENPANNGEPLKPFRIQLAERINRLPQYVLARVNALL
                     HQKRKAGQDVIDLGMGNPSEPPHELVIEKLAEAARDPDNHGYSKANGILNLRREVAAK
                     YFKKHGVRLDPESEVIVTIGSKEGFSHMCLAAIGAGETAIIPAPYFPAHMYAIVMASG
                     NVITLEVNDSEQFLSHVAFTCQHLNPKPKLLVVNYPHNPSSKTVDPEFYVEVVKLARR
                     YGFMVISDFAYADVAFDGYQPPSFLAAPGAKEVGVEFTTMSKGYNMAGWRVGFCCGNA
                     EMVRALGIIKGYYDYGLFQAIQIAAIVALRHTDAAVEAQSRLYQARRDLLLEGLRKQG
                     WQVETPRAGMFCWAKYPEPFASTMTSLDFSMKLLEEANVAVSPGSGFGPAGEGFVRMA
                     LVENENRLRQAVRQMGRVFGEKSGKGSEKVGA"
     gene            1661..1915
                     /locus_tag="VFV87_07570"
     CDS             1661..1915
                     /locus_tag="VFV87_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103650.1"
                     /translation="MRDWVIIWDLDDDPDGNVAHIEEHGISKDEVVEILRDPLSESVS
                     RSSGRPIAFGITSTGKSIGVVFDQVDDDTVYPVTAYEVEF"
     gene            1932..2282
                     /locus_tag="VFV87_07575"
     CDS             1932..2282
                     /locus_tag="VFV87_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5103651.1"
                     /translation="MARKIIQRTRDRYLTPEEAAKYRKIREQIEEEKPEINARIKAQL
                     EAASSMQRVFQQLKQVREAKGLSLRDIQDLTGIDASSLSKLERGERENFTVDTLVRYA
                     QALGKQVAVSVSDG"
     gene            2433..2900
                     /locus_tag="VFV87_07580"
     CDS             2433..2900
                     /locus_tag="VFV87_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103652.1"
                     /translation="MKAQTAIFGDALDFLRRVEDPKRAKMNRREIYSPQKVAAMRKRY
                     PGVPSDYLAYLREVGAGTFRECQFMVYGFLATPDEILGEGVFDWLDAKTRVLCFGDDF
                     SGDMSGFLPDEGWQIVELWHDSGRVRHQNQSFGKYIRAQMLMGPNGDDLRSKS"
BASE COUNT          662 a          841 c          998 g          560 t
ORIGIN      
        1 agtggcgaaa agagcgcaac agcgcaacat ggcagaaaaa cgctaagtcg tttaacggcg
       61 aagcattgca gagaaagttg cgcggcgaaa aagttcgagt tgcgcgcaac attgcgcctg
      121 cgcgtacact gttcacctgc acggcaagtg cgattcgggg ggtgttctgc ggggcgaacc
      181 gatcgcctgc ccagtccctc catcctgccg ccagacttga cgtgccgatg atgggggtgg
      241 gagaacagac aaagtgggga ttcggcaggg aagggcaggt tatagatggc gggtaaaaaa
      301 ttgagcgggc ggattcagca ttccatgtca gacatcgatt cccaatcgga gaatcccgcc
      361 aacaacggcg agcccctcaa gccgttccgc attcaactcg cggagcggat caatcggctg
      421 ccgcagtatg tgctggcgcg ggtcaatgcg cttttgcacc agaagcggaa ggcggggcag
      481 gacgtgatcg accttggcat gggcaacccc agcgagccgc cgcacgagct ggtgatcgag
      541 aagctggccg aggcggcccg cgatccggac aaccacggct acagcaaggc gaacggcatt
      601 ctcaacctgc ggcgggaggt ggcggccaag tatttcaaga agcacggcgt gcggctcgat
      661 ccggagagcg aggtgatcgt cacgatcggc tcgaaggagg gcttcagcca catgtgcctg
      721 gcggcgatcg gggcggggga gacggcgatc atccccgcgc cgtattttcc ggcccacatg
      781 tacgcgatcg tcatggcgtc gggcaacgtg atcacgctgg aagttaacga cagcgagcag
      841 ttcctctcgc acgtcgcctt cacttgccag cacctcaacc cgaagcccaa gctgctggtc
      901 gtcaactatc cgcacaaccc gtcgagcaag acggtcgatc cggagttcta tgtcgaggtg
      961 gtcaagctcg cccgccgcta cggcttcatg gtgatcagcg atttcgccta tgccgacgtg
     1021 gcctttgacg gctatcagcc gccgagcttc ctggcggcgc cgggggcgaa ggaggtgggc
     1081 gtcgagttca ccacgatgag caagggctac aacatggccg gctggcgggt cggcttctgt
     1141 tgcggcaacg cggagatggt gcgggcccta gggatcatca agggctatta cgactacggc
     1201 ctattccagg cgatccagat cgccgcgatc gtcgccctcc gccacaccga cgccgccgtc
     1261 gaggcccagt cgcggctcta ccaggcccgc cgcgacctcc tgctcgaggg cctgcgaaag
     1321 caaggctggc aagtcgaaac gccgcgggcc ggcatgttct gctgggcgaa atatcccgag
     1381 cccttcgcca gcaccatgac gagcctcgac ttctcgatga agctgctcga agaagccaac
     1441 gtcgccgtca gccccggcag cggcttcggc cccgccggcg aaggtttcgt ccgcatggcc
     1501 ctcgtcgaaa acgaaaaccg gctacggcag gcggtgcggc aaatggggcg tgtattcggt
     1561 gaaaaaagtg gaaagggatc ggagaaagtt ggagcgtaga tgacgatttg cctgatttgg
     1621 cgggtgtggt aaaatatccc tacagcaaga attcgctgcc atgcgggatt gggtaatcat
     1681 ctgggatctg gatgacgatc ccgatggcaa cgtagcgcac attgaagagc atgggattag
     1741 caaggacgaa gtcgttgaaa tccttcgcga tccgctgtcc gagtcggtga gtcggtcgtc
     1801 tggaagacca atcgcctttg gcattacgag caccggcaag tcgattggtg tcgtgtttga
     1861 ccaagtcgat gacgacacgg tttacccggt gacagcgtac gaagtggagt tttaaccgag
     1921 gtggatgtcc aatggcacgc aagatcatcc agcgcacgcg cgatcgttat ttgacgcctg
     1981 aggaggcggc caagtaccgt aaaattcgcg agcaaatcga ggaagagaag ccggagatca
     2041 acgcccgcat caaggcgcag cttgaagccg cgtcctcgat gcagcgggtg tttcagcagc
     2101 tcaagcaagt gcgtgaagcg aaaggcttga gcctgcggga cattcaagac ttgaccggaa
     2161 ttgacgcgtc ctcgctttcc aagctggaac gcggcgagcg ggagaacttt acggtcgata
     2221 cgctggtgcg ctatgctcag gcgctcggca agcaggtcgc cgtcagcgtg tccgacggat
     2281 gatgccgtca gccccggcag cggcttcggc ccgaaggctt tgtgcgtatg gccctcgtag
     2341 aaaacgaaaa ccggctaagg caagcggtgc ggcagatggg aagggtggtg ggggagcgga
     2401 gtgggaagga aaaggtgggg gcgtaggaat aaatgaaagc gcaaaccgcc atctttggtg
     2461 acgctctcga ctttctgcga cgtgtggaag atccaaagcg tgccaagatg aatcgcaggg
     2521 agatctactc gcctcagaaa gtggccgcga tgcggaaacg gtatcccggc gtaccgagcg
     2581 attacctcgc gtacctgagg gaggtagggg cgggcacttt cagagagtgt cagttcatgg
     2641 tctacggatt cctcgcgacc cccgatgaaa tccttggcga aggcgtattt gactggctgg
     2701 atgctaaaac gcgtgtactt tgttttgggg atgacttcag tggagacatg agcgggtttt
     2761 tgcccgacga aggctggcaa attgtcgagt tgtggcatga ctccggccgc gtccgacatc
     2821 agaatcagag ctttggcaaa tacatccgcg ctcaaatgct tatggggccg aacggtgacg
     2881 atttgcgaag caagtcataa acttgcacgc gctgcaatcg gcgaatgaaa ttcctgctcc
     2941 aaaccctcgc cttcctctgg gccagccctt acacgctggc gggcctgctc ctcggcagcc
     3001 tcgggctgtg cacaggcggg cgggcgcggg ttcgcgcacc ggccgttgag ttctacggct
     3061 g
//
LOCUS       DASUGB010000395         5755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1294470, whole genome shotgun sequence.
ACCESSION   DASUGB010000395 DASUGB010000000
VERSION     DASUGB010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294470"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2155
                     /locus_tag="VFV87_07585"
     CDS             185..2155
                     /locus_tag="VFV87_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX5103653.1"
                     /translation="MAAAQRDISVSEFFAKNRHLLGFDNPRKALLTTVKEAVDNSLDA
                     CEEAGIVPEIWVTIEATGNNRFKVSVQDNGPGIVKKQIPLIFGKLLYGSKFHRLRMSR
                     GQQGIGISAAGMYGMLTTGKPVKIISKISPKKPAHYFELQIDTKRNQPEIINGKGEGI
                     DIPAGDKGHKMLVEEGIDWVTHYPQASGGREPPEASAKVGPEVKSGTRVTIELEGRFI
                     RGRGSVDEYLQQTAIANPHVTLHYKDCEGNENDYRRSTTQLPPEPKEIKPHPYGVELG
                     RLVTMLKDTRASTTSAFLTESFSRVSSAVAKKICETAKITTRSNPHRIGRQEADSLYQ
                     AIQNTRISAPATDCLVPIGEQLILKGLHQVVPGEFYCAATRPPAVYRGNPFLIEVGLA
                     YGGVSQAQKVSLEALAEMLSESDARTLRQFLISGFDGVGSDAADKILTEAKMGTRQSP
                     AKLKKDEIAQLHAAMRNVNLSEGQSMQVLRYANRVPLQFQPAACAITQSVIGNNWRSY
                     GLSQSRGNLPQGPVTVMVHMASVWVPFTSESKEAIAGYPEIQKELRLGLQAVGRKLGM
                     YLNRRQKVKQEGERRSVFLRYLGEVAGAVGEIQDLNDKKRKDLYDRLLHVAKRKTAEA
                     DTKLDDRGKRIEAGDEDLGENVLIVEQPDGAV"
     gene            2248..3027
                     /locus_tag="VFV87_07590"
     CDS             2248..3027
                     /locus_tag="VFV87_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103654.1"
                     /translation="MWAAFRSVARWLEERQFSYAEIDHLAMFHHGFREYSEQVVLLVE
                     RPALEALRAEFGHSSVAESPWTFIEPETGVPVIVHSEIALSNAEPIRLVDLATGLEAE
                     DGLRFIGLADLVSLKLAARRVDGAVNRGVAEVQQLILALCLTQEFGSTLHAAARESYR
                     KICSAIDASCGNYLLLWESGEEVSQAPTFDELISASPAHSDKLRDMQRDGVTIFSARP
                     SYKGRALLSTNNRAIARKYDMHHESEYLFSDELEPANGNHG"
     gene            3020..4168
                     /locus_tag="VFV87_07595"
     CDS             3020..4168
                     /locus_tag="VFV87_07595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5103655.1"
                     /translation="MAKKSTKGSTATAAPVKFTDKDKKTMGNLRNLAGEVAGFAERSR
                     PPHLEVPSRSLSNVRFNQSKKIIEMGSGTNRRELFNLSQAKAYMQTLLVGSGAKRLIE
                     QGKTTSLRGLYYMLKHTIEGTKEETFDDQSESDTMIEDVEVILNAMREELHLYAKNAG
                     AMVGNITLVDSGDEIDCTRMGSGGYSIPSICEPEIVQFKNCKAKFILHVEKDTVWRRF
                     NEDKFWKEHNCILTHGGGQPPRGVRRLLYRLHHELDLPIYCVLDNDPWGYYIYSVLKQ
                     GSINLAYESKRMAIPAVRYLGLRSRDYDACELSPSVQIKLNEQDIKRANQIKNYPWFA
                     SKKAWQKEIDLMLANGFKLEVESLISKDISYVTEEYVPQRLEEGDFLD"
     gene            4252..4494
                     /locus_tag="VFV87_07600"
     CDS             4252..4494
                     /locus_tag="VFV87_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103656.1"
                     /translation="MIELTEQQAQAVEHPDDFPPRVVNPRTKETFVLLRAAEYERLKE
                     QEYDDSPWTREELQALAWERVKHEDRDEYDALPEKP"
     gene            4491..4841
                     /locus_tag="VFV87_07605"
     CDS             4491..4841
                     /locus_tag="VFV87_07605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5103657.1"
                     /translation="MKRGDVVLVRFPHPSGQRGKKRPAVVVQSDAYASTVSTVVVAEV
                     TKNLTMKGDPACLFVDVSTAEGKASGLLMDSVVSSLVLDTVYADTIAQVLGSLSPTLL
                     QKFDDCVKVGLGLP"
     gene            complement(4856..5395)
                     /locus_tag="VFV87_07610"
     CDS             complement(4856..5395)
                     /locus_tag="VFV87_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103658.1"
                     /translation="MFRKTQPPRQRESFNREIEIDTQPFKELWIIDDGIAKLTVNCKG
                     VAHQLAAANCDSQMSVLSACVQGSASTDETRFAFKGDVLTLQPTRDLVWSGWIMEEED
                     LPDPFVCHLSLERNESNLLDDMRSRYGDHQCRKEHISQGKSRRPPIELVFHGAMHGGN
                     GRGEHSTVALLVVNPELTG"
     gene            complement(5420..>5755)
                     /locus_tag="VFV87_07615"
     CDS             complement(5420..>5755)
                     /locus_tag="VFV87_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103659.1"
                     /translation="SPGAYPLESWPVVPSSFRINTENSADELAIEPIRRLSAPVPGTS
                     VAVFSQTNDSVRGKPPTTAENEQSAADSPPRRRCAPAQNSSAHCSFSRIYNAPVRAAL
                     LQKIAPANA"
BASE COUNT         1295 a         1752 c         1743 g          965 t
ORIGIN      
        1 aaaatcggcc gaccgaccgc tggccaaaac ccggcgcgcg accgacgaat cgacgaacgg
       61 tcacgccgag ccagacgtcg tcgctgaaga aacagcagcg gacgtcgagc ccgagcacga
      121 agccgagcac gagcacgaac atcatgctgc gcacaagcgc cgcaagcgcg ccacggccca
      181 atcgatggcc gccgcgcagc gcgacatttc agtcagcgaa ttcttcgcca agaatcgcca
      241 cctgctgggc ttcgacaacc cgcgcaaggc cctgctgacg accgtcaagg aagcggtcga
      301 caactcgctc gacgcctgcg aggaagcagg catcgtgccg gaaatctggg tcacgatcga
      361 agccaccggc aacaaccgct tcaaggtcag cgtgcaggac aacggcccgg gcatcgtcaa
      421 gaagcagatc ccgctgatct tcggcaagtt gctctatggc agcaagttcc accgcctgcg
      481 aatgagccgc ggccagcaag gcatcggcat ctcggcagcc ggcatgtatg gcatgctgac
      541 gaccggcaag ccggtgaaga tcatcagcaa gatttccccc aagaagccgg cgcactattt
      601 cgagctgcag atcgacacca agcgcaacca gccggagatc atcaacggca agggcgaagg
      661 gatcgacatc ccggccggcg acaaaggcca caagatgctg gtcgaggaag gcatcgactg
      721 ggtcacgcac tacccgcagg cgagcggggg gcgtgagccc cctgaggcgt ccgccaaggt
      781 tgggccggag gtgaaaagcg gcacgcgcgt gacgatcgaa ctcgaaggcc gtttcatccg
      841 cggccgcggc agcgtagatg aatacctgca gcaaacggcg attgccaatc ctcatgtgac
      901 gctgcattac aaagattgcg aggggaatga aaacgactac cgccgctcga cgacgcagct
      961 tccaccggaa cccaaggaaa tcaagccgca cccgtacgga gtggagctcg gccggctagt
     1021 cacgatgctg aaggacacgc gggcgagcac gacttcagcg ttcttgaccg agtcgttctc
     1081 gcgggtcagt tcggcggttg cgaagaagat ttgcgaaaca gccaagatca caacgcgctc
     1141 caatccgcat cgcatcggcc ggcaggaggc ggactctctc tatcaggcca tccagaacac
     1201 gcgaatcagc gcgccggcga ccgactgcct ggtgccgatt ggtgaacagc tcatcctgaa
     1261 gggcttgcac caggtcgtgc ccggcgaatt ctactgcgcg gccacgcgcc cgccggctgt
     1321 ctatcgcggc aatccgttct tgatcgaagt gggtctggcc tacggcggcg tttcgcaggc
     1381 gcagaaggtc tcgctcgaag cgctggccga aatgctcagc gaaagcgatg cccgcacgct
     1441 gcggcagttc ctgatcagcg gcttcgacgg cgtcggcagc gacgcggcgg acaaaatcct
     1501 gaccgaagcc aaaatgggca cgcggcaaag cccggcgaag ctgaagaagg acgaaatcgc
     1561 ccagcttcac gcggcgatgc ggaacgtaaa cctcagcgaa gggcagtcga tgcaggtcct
     1621 ccgctacgcc aaccgcgtcc ccctccagtt ccagccggcg gcctgtgcga tcacgcaatc
     1681 ggtcatcggc aacaactggc ggtcgtacgg cctctcgcaa tcgcgcggca acctgccgca
     1741 aggcccggtc acggtcatgg tccacatggc cagcgtctgg gtccccttca ccagcgaatc
     1801 gaaagaagcg atcgccggct atcccgagat ccagaaggag cttcgcctcg gcctccaagc
     1861 cgtcggccgc aagctcggca tgtacctcaa ccgccggcag aaggtaaagc aggaaggcga
     1921 gcgccgctcg gtcttcctcc gctatctcgg cgaagtcgcc ggcgccgtcg gcgaaattca
     1981 ggatctgaat gacaagaagc gaaaggacct ctacgaccgc ctgctgcacg tcgccaaacg
     2041 caaaacggcc gaagccgaca ccaaactcga cgaccgcggc aagagaatcg aagccggcga
     2101 cgaggacttg ggcgagaacg tgctcatcgt cgagcagccg gacggagcag tttgatggcc
     2161 aggccggaaa cgattcgcga gcgcctgaat ggcaatctgc aactcgcgct tcgcgaagcg
     2221 gacctctatt tcgcacgaca aggctgcgtg tgggcggcgt ttcgaagcgt cgcgcgctgg
     2281 ctggaggaac gtcaattcag ctacgcagag attgaccatt tggcgatgtt tcaccacggc
     2341 tttcgagagt actcggagca ggtcgtacta ctcgtcgagc gcccggcact tgaagcgcta
     2401 cgtgcagagt ttggtcattc cagcgtcgcc gaaagtccgt ggacattcat cgagccggaa
     2461 accggcgtgc ccgtgattgt tcactcggaa attgcactat cgaatgcgga gcctattcgc
     2521 cttgtcgatc tcgcaacggg cctcgaagct gaggacggtt tgcgattcat tggccttgca
     2581 gacctcgtga gcctcaagct cgccgctcgc cgggtcgatg gcgctgtgaa ccgcggcgtc
     2641 gcagaggttc agcagttgat cttggcgcta tgcctgactc aagagtttgg cagtacgctt
     2701 cacgccgcag cccgcgagtc gtatcgaaag atttgcagcg ctatcgacgc atcgtgcggg
     2761 aactaccttc tcctctggga aagcggggaa gaagtctcgc aagctccaac attcgacgaa
     2821 ctcatctcgg cgagtcccgc acactccgac aagcttcgcg atatgcagcg cgacggcgtg
     2881 acgatctttt cggcgcgacc cagctacaaa ggccgagcgc tcctcagcac caacaatcgc
     2941 gcgatcgccc gaaaatacga catgcatcac gagtccgaat acttgttctc cgacgaactc
     3001 gaacctgcca acggaaatca tggctaagaa atccaccaag ggatcgaccg ccaccgccgc
     3061 cccggtcaag ttcaccgaca aagacaagaa aacgatgggc aacctgcgga atctcgcggg
     3121 ggaggtcgcg ggatttgccg aacggagccg gccgccgcat ttggaggtgc ccagccgctc
     3181 gctctcgaac gtccggttca atcagagcaa aaagatcatc gagatgggga gcggcaccaa
     3241 ccgccgcgaa ttgttcaatc tgtcgcaggc caaggcgtat atgcagacgc tcctggtcgg
     3301 cagcggggcc aagcgcctga tcgagcaggg caagaccacg agcctccgcg gtctgtacta
     3361 catgctcaag cacacgatcg aagggaccaa ggaagagacg ttcgacgacc agagcgagtc
     3421 ggacacgatg atcgaggacg tcgaggtgat cctcaacgcg atgcgcgagg agctgcacct
     3481 ttatgccaaa aacgccgggg cgatggtcgg caacatcacg ctcgtcgata gcggcgacga
     3541 aatcgactgc acccgcatgg gcagcggcgg ctacagcatc ccgtcgatct gcgagccgga
     3601 aatcgtgcag ttcaagaact gcaaggcgaa gttcatcctg cacgtcgaaa aggacacggt
     3661 ctggcggcgg ttcaacgaag acaaattctg gaaggagcac aactgcatcc tcacgcacgg
     3721 cggcggacag cccccgcgcg gcgtccggcg gctgctgtat cggctgcacc acgagctgga
     3781 cctgccgatt tactgcgtgc tcgataacga cccgtggggc tactacatct acagcgtgct
     3841 caagcagggc tcgatcaacc tggcctacga gtcgaagcgg atggcgatcc cggccgtccg
     3901 ttatctcggc ctccgcagcc gcgactacga cgcctgcgag ctctcgccca gcgtgcagat
     3961 caagctcaac gagcaggaca tcaagcgggc caaccagatc aaaaactacc cctggttcgc
     4021 cagcaagaaa gcctggcaga aggaaatcga cctgatgctc gccaacggct tcaagctgga
     4081 agtcgaatcg ctgatcagca aggacatcag ctacgtgacc gaagaatacg tcccccagcg
     4141 gctggaggag ggggattttc tggattgagc gattcccatc gccgtagatc ggacacgtct
     4201 gcgttggccc gacctgccag ggcacggtag gataacagaa cggaggcaga catgatcgaa
     4261 cttaccgaac agcaggcgca agccgtcgag caccccgatg atttcccgcc gcgtgtcgtc
     4321 aatccccgca cgaaggagac gttcgtcttg ctccgcgctg ccgagtacga gcgcctgaag
     4381 gaacaagagt acgacgatag cccgtggacg cgcgaagagc ttcaggccct ggcttgggag
     4441 agggtcaagc acgaggaccg ggacgagtac gatgccctgc cggagaaacc atgaaacggg
     4501 gcgacgtagt ccttgtccgc tttccgcatc cctccgggca gcgcggcaag aagcgccccg
     4561 cggtagtggt ccaatcggac gcctatgcca gcacggtcag cacggtcgtc gttgcggaag
     4621 ttacgaaaaa cctgacaatg aagggcgatc ccgcctgcct gttcgttgac gtgagcacgg
     4681 ccgagggcaa ggcgtccgga ctactcatgg attcggtcgt ttccagcttg gtgctggaca
     4741 ccgtttacgc cgataccatt gctcaggtgc tggggagtct ttcaccgacg ctgttacaga
     4801 agtttgacga ttgcgtgaaa gtcggattgg ggctgccttg agtaggaggc ggcagctaac
     4861 ccgtcagctc tggattgacg acgagcaatg cgacggtact gtgctcgcct cgcccattgc
     4921 ctccatgcat cgcgccatga aaaacaagtt caatcggtgg acgccgcgat ttaccctgcg
     4981 aaatatgctc cttgcggcat tggtgatcgc cgtaacgact gcgcatgtcg tccagcaagt
     5041 tcgactcgtt tctctccaga gacagatggc acacaaaagg gtcaggcaaa tcctcctctt
     5101 ccatgatcca tcccgaccag accaaatcgc gggttggctg gagggtaagt acgtctcctt
     5161 tgaaggcaaa gcgagtttca tcggtcgaag cggaaccttg aacgcacgcg ctgagaacgg
     5221 acatctggct atcacaattg gcagccgcca gttgatgtgc cacacccttg caattgacag
     5281 tgagtttcgc aatcccgtcg tcgatgatcc acagttcttt gaacggctgg gtatcgattt
     5341 cgatttcccg attaaatgat tccctttggc ggggtggctg ggtcttgcga aacatgaccc
     5401 agccgacatg acgatcgact caggcgttgg ccggagcaat tttctggagt agcgcggcgc
     5461 ggactggggc gttgtatatt cggctgaatg agcaatgcgc cgacgaattt tgcgcaggtg
     5521 cacagcgacg acgcggcggg gaatcggctg cggattgctc attttctgct gtggttggcg
     5581 gcttcccgcg cacggaatcg ttcgtctgcg agaacacggc aacggaggtt cccggcaccg
     5641 gcgcggacaa cctgcggatc ggctcgatgg caagctcgtc ggctgaattc tcggtgttga
     5701 tccgaaacga ggacggcacc accggccagc tctcgagggg atatgcgccc ggcga
//
LOCUS       DASUGB010000396         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1017670, whole genome shotgun sequence.
ACCESSION   DASUGB010000396 DASUGB010000000
VERSION     DASUGB010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017670"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..151
                     /locus_tag="VFV87_07620"
     CDS             8..151
                     /locus_tag="VFV87_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5103660.1"
                     /translation="MTTWLGLVVAIPALIAFSYFRNKIDSIVAECGKRVERVLMPLSR
                     KRM"
     gene            205..621
                     /locus_tag="VFV87_07625"
     CDS             205..621
                     /locus_tag="VFV87_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5103661.1"
                     /translation="MKLNKHRRSHQLLVNMTPMIDIVFLLIIFFITVNQVTEANRTKM
                     ELPTLQGSQDQSKAALTINVDEAGEIIIAGQAVSLADLVRIVSAELAGVDNEPSRLTV
                     VLRADRRGNSRTVNEVIRLLTRLDIGRVRFSVEKPA"
     gene            618..1052
                     /locus_tag="VFV87_07630"
     CDS             618..1052
                     /locus_tag="VFV87_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5103662.1"
                     /translation="MRLTSRSGRHSSRIEVQMASMIDVVFLLLIFFMVTMSFRKTERE
                     LDPAVKVQKASTSQAASDLAPPIVEIVRGSSGGFVYKLGGRELTAAGELTEVLRQFEN
                     KLDGAIVRAQNEAPWDMAASAIQACKDARFSLVTYQPLDRGP"
     gene            1059..>3291
                     /locus_tag="VFV87_07635"
     CDS             1059..>3291
                     /locus_tag="VFV87_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103663.1"
                     /translation="MKSRTTLALLLVALAAGFVWNTASSADDQALEELLSRLNLSDLR
                     LHHMERQLERLPAGDGRLPAAKRLADAYAEHLIAAADDAEKFAALKARIEKLLAEIPD
                     ARTPSVEVVLLQAEYQRAEALMIRWLDEPADRAPLAEADEVLTRIAPLLIAYQKEITA
                     AADKAAEELDRLKSEQARAAAERQVARQQAIAARSGYFAGWAAYYHGVARQNVAEAQP
                     DFAAAKEQFSIVLDVTDEKNYEPVDVESLGLDSIWRSRAAIGLGLVELGLNRPAAAAK
                     VFAWLDHASVPPVIRDQAAYWHVQGMLNVGLAAEAGQFVETQVEGFTGSPSPGKNSVC
                     IAAIRAGAALGDGKKDQRQKLVEQGIRGLARMRQFETLDKLIEKHNLAAMADAGSFYL
                     TWLRGRQQFLAAEKTKAPADFQAAAKTLATALSHPLAQRDLTDAGQARYYLGWSQYRL
                     EDLPAAARSFQEAATALKSSLADQAVQAAWMQCTCLIQLAQQDKKHVTAAIAALQNLK
                     QDFPASKQAGRADLLITRLRQSHSSPEEAIRELATVPAGDPNYVSAQYEICQLQYQLW
                     SRARTPLSPGGRGAGGEGAMAAAIAAELLTTADRFLAGAVEEGDWERKLKAALLALDV
                     LWSQSPPNQQRIEALLREAAKAAENLDPSNAALVEYHYRRLQLAQKSGDKETLQAAAQ
                     WISKNGVGSPYELAALVIAARAADDAAESAAAADRTARREEAARIYARLVDLLGDSPA
                     TLAS"
BASE COUNT          612 a         1074 c         1059 g          546 t
ORIGIN      
        1 ggcgctcatc acgacgtggc ttggcctcgt cgtggcgatt cccgcactga tcgcgttcag
       61 ctacttccgc aacaagatcg attcgatcgt tgcggaatgc ggcaagcggg tggaacgggt
      121 gctgatgccg ttgtcccgaa agcgcatgta gggtgggctg tgcccacctc aagcgaatcg
      181 gtgggcacag cccaccctac gaccatgaag ctcaacaaac accgtcgcag ccaccagctt
      241 ctggtgaaca tgacgccgat gatcgacatc gtgttcctgc tcatcatctt cttcatcacg
      301 gtcaaccagg ttaccgaagc caaccgcacg aagatggaac tgccgacgct gcaggggagc
      361 caggaccaat cgaaggccgc cctgacgatc aatgtcgatg aagcgggcga gatcatcatt
      421 gccggccagg cggtttcgct cgccgatctg gtgcggattg tctcggctga gctggcgggc
      481 gtcgataacg aaccgagccg attgaccgtc gtcctgcggg ccgatcgccg cgggaattcc
      541 cgcacggtca acgaggtcat ccggctgctt acgcggctgg atattggccg ggtccgcttc
      601 agcgtggaga agccggcatg agactcacca gccgtagcgg ccgacattct tcgcggatcg
      661 aagtgcagat ggcaagcatg atcgacgtcg tgttcctctt gctgattttc ttcatggtga
      721 cgatgagctt tcgcaagacc gaacgcgagc tcgaccccgc ggtgaaggtc cagaaagcgt
      781 ccacgtcgca ggcggcgagc gacctggcgc cgccgatcgt cgaaatcgtc cgcggcagct
      841 cgggcggatt tgtttataag ctcggcgggc gggaattgac cgcggccggc gagttgaccg
      901 aggtactgcg gcaattcgaa aataaacttg acggggcgat tgtgcgggct cagaacgagg
      961 cgccctggga tatggcggcc agcgccattc aggcctgcaa agacgcccgc ttttcgctcg
     1021 tcacgtacca gccgctcgat cgcgggcctt aacgcatcgt gaaatctcgc accactctcg
     1081 ctctgctgct cgtcgcgctg gccgcgggat ttgtctggaa tactgcatcg tcggctgacg
     1141 accaggcgct agaggagctg ctctcgcggc tgaacctgag cgatctgcga ctgcaccaca
     1201 tggagcggca gcttgagcgc ctgccggctg gcgatgggcg gctgcccgcg gccaaacgcc
     1261 tcgccgacgc ctacgccgag cacttgatcg ctgccgcgga cgacgccgaa aagttcgccg
     1321 ccctcaaagc caggatcgaa aagctcctgg cggaaatccc ggacgcccgc acgccctcgg
     1381 tcgaggtcgt cctgctgcaa gccgagtatc agcgggccga agcgctgatg atccgctggc
     1441 tcgacgagcc ggccgaccgc gcgccgctgg cagaagccga cgaagttctg actcgcatcg
     1501 ccccgctgct gatcgcctat caaaaggaga tcacggccgc tgccgacaag gccgccgaag
     1561 agctggacag gctcaagagc gagcaagccc gcgccgctgc cgagcggcag gttgcccggc
     1621 agcaggcgat cgctgcacgc tccggctatt tcgccggctg ggccgcctac tatcacggcg
     1681 tcgcccggca gaatgtcgcc gaggcacaac ccgatttcgc cgcggccaaa gagcagttca
     1741 gcatcgtgct tgacgtgacc gacgagaaga actatgaacc ggtcgatgtc gagagcctgg
     1801 ggctggactc gatctggcgc tcgcgagctg cgattggact gggcctggtg gaactcggct
     1861 tgaaccgtcc cgcagcggcg gcgaaggtct ttgcctggct cgatcacgcc agcgtgccgc
     1921 ccgtgatccg cgaccaggcc gcctattggc acgtgcaagg gatgctcaac gtcggcctcg
     1981 ccgccgaagc ggggcagttc gttgaaacgc aggtcgaagg cttcaccggc agcccgtcgc
     2041 ccggcaaaaa cagcgtctgt atcgcggcga tccgggccgg cgctgcactc ggtgatggaa
     2101 aaaaggacca gcggcagaag ctcgtcgagc agggcattcg tggcctcgcc cggatgcggc
     2161 agttcgagac gctcgacaag ctgattgaaa agcacaatct ggccgcaatg gccgatgccg
     2221 gcagcttcta tctcacgtgg cttcgcggcc ggcagcaatt cctggccgcg gagaagacga
     2281 aggccccggc ggacttccag gccgcggcga aaacgcttgc caccgctctg tcccatccgc
     2341 tggcccagcg cgatctcacc gatgccggtc aggctcgcta ctacctgggc tggtcgcagt
     2401 accggctgga agatttgccg gcggcggcac gctcgtttca agaggcggcg acggctctca
     2461 agtcgtcgct ggccgatcag gccgtgcaag cggcctggat gcagtgtacc tgcctgattc
     2521 agctcgctca gcaggacaag aagcacgtca cggccgccat cgccgccctg caaaacctca
     2581 agcaggattt tcccgcctcc aagcaagccg gccgggccga cctcctgatc acgcggctga
     2641 ggcagagcca ttcgtcgccc gaagaagcca tccgcgaact ggcgacggtg ccggcgggcg
     2701 accccaacta tgtttcggcc cagtacgaga tttgccagct tcagtatcag ctctggtcga
     2761 gggcccgcac tcccctctcc cctggtggga gaggggctgg gggtgagggg gcaatggcag
     2821 cagcaatcgc cgcggaactg ctgacgactg ccgatcgctt cctcgcgggc gcggtcgagg
     2881 agggcgactg ggaacgcaag ctcaaggccg cgctgctcgc cctcgatgtg ctgtggagcc
     2941 agtcgccgcc caaccagcag cggattgagg ccttgctccg cgaagccgcc aaggctgccg
     3001 agaatctcga tccatccaat gcagccctcg tcgaatatca ctaccgccgc ctgcagctcg
     3061 cgcagaagtc gggcgataag gagacgctcc aggctgcggc ccagtggatc tcgaaaaacg
     3121 gcgtcggctc gccgtacgag ctggcggctc tggtgattgc cgcccgcgcc gccgatgacg
     3181 ccgcggaatc cgccgccgca gccgaccgca ccgcccgccg ggaagaagcc gcccgcattt
     3241 atgcccggct ggtcgatttg ctgggtgatt cgccggcgac gctcgccagc a
//
LOCUS       DASUGB010000397         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1155703, whole genome shotgun sequence.
ACCESSION   DASUGB010000397 DASUGB010000000
VERSION     DASUGB010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155703"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..610)
                     /locus_tag="VFV87_07640"
     CDS             complement(74..610)
                     /locus_tag="VFV87_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103664.1"
                     /translation="MESRLPDYQAAIDDALQLVYSQHHRLIGQLHPESFRELSLEQLR
                     EGPLGRDLQKLALLARGGSHESKELVLSAIDSVLQILFWPVAADDYTVPRTFWDTELG
                     RMLALAKYRAYDATELVSIGSAAQQLGVTRPTIYRWMDDRTLNYVRDDMSGRTFVVRR
                     DIDNLKKVAAELSAQGDI"
     gene            complement(1093..1425)
                     /locus_tag="VFV87_07645"
     CDS             complement(1093..1425)
                     /locus_tag="VFV87_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103665.1"
                     /translation="MTVMLYMAALGIWGVVAYLRGQLVSGSFAGAMVIGEIVVVAQVL
                     AGLALFAEGARPPTATHYLYGVSAILVLPFAWSIYRERDQRQAVLIYGLVSLFVFGLA
                     VRGIMTGR"
     gene            complement(1564..>2409)
                     /locus_tag="VFV87_07650"
     CDS             complement(1564..>2409)
                     /locus_tag="VFV87_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5103666.1"
                     /translation="RGPGRNLGESSVFEVTSLGSGSSGNALLVRTSTATLLVDCGVTI
                     RRLTGGLASLGLTLADVDAVLVSHEHVDHVRELSRFTSLGATVVCTRGTANASQAPRA
                     SCQVVAPRRYVELAGIEIRAIPVSHDAAEPCGFFLRASAGSLVVATDLGRSGPELIEA
                     VAAADLVVLEANHDEHLLRRGPYPPQLQRRILSDAGHLSNEACAGILQTALSPSHRLP
                     TVWLAHLSETNNRPGLAKQTVERRLGQIGIRLDLRALPRRDVGPCWTPKTRRAGVAQL
                     PLDLT"
BASE COUNT          472 a          736 c          789 g          412 t
ORIGIN      
        1 cgtcagggac ggccatgttg tcgcggtcaa cgacggttga ttgctgaccg acgccaactg
       61 aagcgcgagt gcgtcagata tcgccctgag ccgagagctc ggccgcgacc ttcttcaagt
      121 tgtcaatgtc tcggcgcacg acgaacgtgc ggccgctcat gtcgtcgcgt acatagttga
      181 gagtgcgatc gtccatccag cggtaaatcg tcggccgagt cacgccgagc tgctgcgcag
      241 cgctgccgat gctgaccagt tccgttgcat cgtaggcgcg atacttggca agggcgagca
      301 tgcgcccgag ctcggtgtcc cagaacgtgc ggggcacggt gtagtcgtca gcagcaaccg
      361 gccagaaaag aatctggagc accgagtcga tggcggacag caccaactcc ttcgactcat
      421 gagagccgcc gcgtgccagg agggcaagtt tctggagatc ccggccgagg ggtccctcac
      481 gcaattgctc cagggaaagc tcgcgaaacg actcggggtg aagttgaccg atgaggcggt
      541 gatgctgact atagacgagc tggagtgcgt cgtcgatcgc cgcctgatag tccggcagcc
      601 ttgactccac gggttctcct ttcatccgtg cggcaaaacc ggctctacag cgacgggaaa
      661 gcgagtacgg tcagagtgta cgctcatgca ctgagagggt caacaataca ctgtccgttc
      721 aacagaaacc gggactcttg gcaacggata tttgcctcta tgcgttgaaa atcttgagtc
      781 tgtgaccgat tggcacttct ctgtctcccg gccgagcccg cagagctgaa aggccatctt
      841 ggcgctccgg aaccgtgggc aaggcaggtc gttccgaggg gtcattctcc agagtggcgc
      901 cactgtggca tgaacaaccg tgcttttcac agccccttgg gaggagtctc gaagcaagtg
      961 gcccgactgc tcggagcggc gagatttctc gtaatctcgg aatgacacac gctctgcatg
     1021 cgctctagtc cacatggtag tgggaatccg agagagcaga atgccggagc catcgattcg
     1081 cggaacacgt tgttatcttc cggtcattat tcctcgaacg gccaatccaa agacgaacag
     1141 cgagacgaga ccgtaaatca ataccgcctg gcgctgatcg cgctcgcgat agatgctcca
     1201 ggcgaacggg aggacaagga tggcactgac gccgtagagg tagtgcgtgg cggtcggcgg
     1261 gcgcgcgcct tcggcgaaca acgccagccc agcgagcact tgcgcgacga ccacgatctc
     1321 cccgatgacc atcgcgccgg caaaacttcc tgagaccaac tggccgcgga gataggccac
     1381 cacgccccag attcccaggg ccgccatata gagcatgacc gtcatcgaga ggcggtcatg
     1441 cagaaggagc agcgtctcca gggcgcatcg acctttcgct ccggctggac caagcgttac
     1501 ccgcgatccg gcttgcggcg agtatagggt ggccgcatcg tgactccagc ttcgccgacg
     1561 aaatcaagtg agatcgagcg gtaactgcgc cacgccagcg cggcgcgttt tcggggtcca
     1621 gcacggaccc acgtctcgcc gtggcaaggc gcgcaggtcg agtcggattc cgatctggcc
     1681 cagccgtcgc tccaccgtct gtttggcgag cccgggccgg ttgttcgtct cggagaggtg
     1741 cgccagccag actgttggca ggcgatggct tggcgaaagt gcggtttgca ggatgccggc
     1801 gcacgcctcg ttggagaggt gccctgcgtc ggagaggatg cggcgctgca attgcggcgg
     1861 gtaagggccg cggcgcagca ggtgctcgtc gtgattggcc tccaagacga ccaggtccgc
     1921 cgcggcgacg gcttcgatca actcagggcc ggaccggccg aggtccgtgg caacgaccag
     1981 gctccccgcg ctcgcccgca ggaaaaaccc gcacggctcg gcggcgtcgt gcgagacagg
     2041 gatcgcgcgg atctcgatgc cagccaattc gacataccgc cggggtgcga ccacttggca
     2101 gcttgcccgc ggcgcctggc tagcgttggc cgtcccgcgc gtgcagacca cggtagcgcc
     2161 gagtgacgtg aagcggctca actcgcggac atggtcgacg tgctcatggc tgacgagcac
     2221 agcgtccacg tcggcgaggg tcagcccgag cgatgccaag ccgccggtca gccgacgaat
     2281 ggtgacgccg cagtcgacga gcagggtcgc ggtcgaggtg cgaacgagca acgcgttgcc
     2341 ggaactgccg cttcccaggc tagtgacttc gaacacggag gactccccga ggttgcggcc
     2401 aggacctct
//
LOCUS       DASUGB010000398         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1849975, whole genome shotgun sequence.
ACCESSION   DASUGB010000398 DASUGB010000000
VERSION     DASUGB010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849975"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFV87_07655"
     CDS             <1..202
                     /locus_tag="VFV87_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103667.1"
                     /translation="LFRAPDGGWQVFAATSGRAQLTPVEVGLLNDDAAEIVSGLSEGD
                     LVVLAPESSLTEGARIKAVVRQ"
     gene            complement(231..>2235)
                     /locus_tag="VFV87_07660"
     CDS             complement(231..>2235)
                     /locus_tag="VFV87_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX5103668.1"
                     /translation="GSAGQIEQIRAWLKQKGELLDQETIATMQRSNVRLLPATGRSAR
                     FALEQLELLWPATGRSNKLNIRTPSNPNQVPTSAPWNSLSEPRIDSLDPRGPGIDRPL
                     YVPPRRPGALEVERPAAEAEQPAVEPEAPRPQPAVRPSGRAVLVPILRPAEAKKAAEA
                     KAAAKPQPEVTRRSLNAPRGTHFVAAPLAVAQAEPASEAQQPEKSKEPAPADPAAARE
                     PAPEAPPTAAPAEAPPAESTPGSPAATAPMTTKVVPGKPGAPEVVISVGPNGIMIASE
                     DLDALDEIESQLQMMLESRANSVEFNIYYLRYAKADVAASLLTEIVTGAAPSEDGGGG
                     SLMGDLASQMIGGMGGDLLGGILGGGGGGGTSLTSGTMRITPDPRLNALIVQASSRDL
                     DSIEQLLQIIDQPQGPETVETTPVPKFIPVLYGSADEISTIVKQVFASKIQGESGGQQ
                     RQPSPEDFIRALRGGGRGGNSRQQNRGEEQKMTIGVDTKSNSLIVSAPDYLFTQVRDL
                     VTQLDEMASSTRSEETVRVVNLKRASTDLVQKSLVSVLGTSATVNKTGTTGTTSSSSN
                     TSSQTTRTSNGDSRPDNPSADSMRGVMMMQEMMRRSQDGGSSRGFGGSPFGGGDRGSS
                     RGGFGGGSPFGGGSPFGGGSSRGGFGGGSPFGGGGFGGSRGGR"
BASE COUNT          309 a          759 c          763 g          404 t
ORIGIN      
        1 cctgttccgc gccccggacg gaggctggca ggtctttgcc gccaccagcg gccgggcgca
       61 gctaacgccc gtcgaagtcg gcctgctgaa tgacgacgca gccgagatcg tctccggcct
      121 ctcggaaggt gatctcgtcg tcctcgcgcc cgaaagctcg ctcaccgaag gcgcacggat
      181 caaggcggtc gtccgccaat agcaatcaat tcagtcgcgc gttccagccg ctatcggccg
      241 ccgcggctcc cgccgaaacc accgccgccg aaggggctgc caccgccaaa gccgccgcgg
      301 ctgctgccgc cgccgaaggg gctgccaccg ccgaaggggc tgccaccgcc aaaaccaccg
      361 cgactgctgc cgcggtcgcc gccgccaaag gggctgccgc caaatccgcg gctgctgccg
      421 ccgtcctgcg agcggcgcat catttcctgc atcatcatca cgccccgcat cgagtccgcg
      481 ctcgggttat cgggacggct gtcgccgttg ctggtgcgcg tggtctgcga ggacgtgttc
      541 gacgaggaag aagttgtgcc ggtcgtgccc gtcttgttca cggtggcact ggtgcccagg
      601 accgaaacga gcgacttctg caccaggtcg gtgctggccc gcttcaggtt gacgacgcga
      661 actgtctctt ccgagcgggt gctcgaggcc atttcgtcga gctgtgtcac caggtcccgc
      721 acctgcgtga acaggtaatc cggagccgat acgatcagcg aattgctctt ggtatcgacg
      781 ccgatcgtca tcttctgctc ttcgccgcgg ttctgctgcc ggctgttgcc accccggccg
      841 ccgccgcgca gggcgcggat gaagtcctcg ggcgatggct gccgctgctg gccgccgctc
      901 tccccctgaa tctttgaagc gaacacctgc ttgacgatcg tcgaaatctc gtccgccgag
      961 ccgtacagga ccggaatgaa ctttggcacg ggggtcgttt ccacggtctc cgggccctgc
     1021 ggctggtcga tgatctgcag gagctgctcg atcgaatcca gatcgcgcga cgaagcctgg
     1081 acgatcaggg cgttgagccg cggatcgggc gtgatccgca ttgtgccgct ggtgaggctc
     1141 gttcccccgc cgccgccccc gcccaggatg ccgccgagca gatcaccccc catgccgccg
     1201 atcatctgcg aggccagatc acccatcagg ctgccgccgc ccccatcttc ggacggcgcg
     1261 gcgccggtta cgatctcggt gagcaggctg gcggccacgt ctgccttggc gtagcggagg
     1321 tagtagatgt tgaattcgac gctgttggcc cggctctcga gcatcatctg cagttgggat
     1381 tcgatctcgt cgagggcgtc gaggtcttcc gaggcgatca tgatgccgtt gggaccgacc
     1441 gagatcacga cctccggcgc tcctggcttg ccgggaacga ctttggttgt catcggcgcg
     1501 gtcgcggcag gtgagcccgg agtggactcg gcggggggcg cctcagcggg tgctgccgtt
     1561 ggaggcgctt cgggcgcggg ttcacgcgcg gcggccggat cggccggtgc gggctctttc
     1621 gacttttcgg gttgctgcgc ttcgctagcc ggctccgcct gagcaacggc cagcggcgcg
     1681 gcgacgaagt gcgtgccgcg cggcgcgttc aggctgcggc gggtcacttc cggttgcggt
     1741 ttcgccgcgg ccttggcctc cgcggctttc ttggcttccg cggggcgaag gatcggcacg
     1801 aggaccgccc ggccgctggg ccggaccgcc ggttgcggcc ggggcgcttc gggctcgact
     1861 gctggctgct cagcttcggc agccggccgc tcgacttcca gagcgcccgg gcggcgcggc
     1921 ggaacataaa gcggccggtc gattcccgga ccccttggat cgagcgagtc gatccgcggc
     1981 tcgctgagcg aattccaggg cgcgctagtg ggaacctggt tcgggttcga tggagtgcgg
     2041 atgttgagct tgttcgatcg gcccgttgcc ggccatagca gctccagttg ctccaacgca
     2101 aagcgggccg agcggcccgt cgccggcagc aagcggacgt tcgaccgctg catcgtggca
     2161 atggtctcct gatcgagcag ttctcccttc tgcttgagcc aggcgcgaat ctgctcgatc
     2221 tggccggccg agccg
//
LOCUS       DASUGB010000399         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_183590, whole genome shotgun sequence.
ACCESSION   DASUGB010000399 DASUGB010000000
VERSION     DASUGB010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183590"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFV87_07665"
     CDS             complement(<1..277)
                     /locus_tag="VFV87_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5103669.1"
                     /translation="MAFVNFSLLLGGAFVAIPVVLHLIMRQKPRPFVFPAVRFIQQKR
                     MANQRRLQLRHWILLALRCGVIGLLVLALARPSIASAALSNWIALSAV"
     gene            678..>2277
                     /locus_tag="VFV87_07670"
     CDS             678..>2277
                     /locus_tag="VFV87_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103670.1"
                     /translation="MRWDWRNRNKRTGKRASRWNTVARGLALVGLIALAAHWAGRWSA
                     SPYASPGLSSAAAQEAVKPDDPLAKGDLPAAGDQERGARIVRFDVPLPIDGLVDSHIQ
                     SRVEQALRKLPEGGPRPTFVFEFRPKNNTAGEGNDFERALSLARYLCGDRLAGVRTVA
                     WLPRSVKGHAVLPVLACEQIIVQKEAELGAAGADERGAIDALTRRCYQEIADRRKTVP
                     TAIALGFLDKELTVYKVKTPDGLRYELDDDLRSLRQEGKITSQETLFQPGDPHVLSGA
                     QLRELGFASHLADDRRTLAAALDVPMAALQQQLVPEDGWKPLRLDLNGPVHRQAVNFL
                     LRTIEDHERRRDFNLLVVCIQSGGGDLSQSVRLAEKIASLGPQLHSVAVVDSQARGDA
                     VLVAVACDELLVTADAMLGGPGERALSREELTDLRGPLPGIAHAADRDWSLPLALVDS
                     QVQVFRTTRTVGGEVRYLSPDELETLPDREQWNRDDQPLPTTNGITGQSAVEWGLARA
                     VVHNLDDAMSLFQIEGELPTARPNW"
BASE COUNT          407 a          745 c          749 g          376 t
ORIGIN      
        1 cgacggctga cagggcaatc caattcgaga gggcggctga cgcgatgctc ggccgagcca
       61 aggccagcac caacagcccg atcacgccgc accgcagcgc cagcagaatc cagtggcgca
      121 attgcagccg ccgctggttg gccatccgct tttgctggat gaagcggaca gcggggaaga
      181 cgaacggccg cggcttctgc cgcatgatca agtgcagcac gacggggatt gcgacaaaag
      241 cgccgccgag cagcagagaa aaattgacga atgccatgcg aaggagcgtg ggcgttccgt
      301 ggttgtcaga ggccagtggt cagtagttag tggtcagtag tctgaggcca gtggtcagtg
      361 ccttcgtcct tcatccttcc tccttcctcc ttcctccttc ctcccccgcc tccgccatca
      421 taccggcttg cgaaccgggt cggcgccgct atgcctttgt catgtcattt taggcgcatg
      481 gggctgcgtt ggcccaccat ttgccatcca acgtgcggcc tgccgtagcg aataacggtg
      541 tggagggctg gccaatccag ctatccaatg catcggctgg ttgcaacctc ctcgccaagc
      601 tggggttaag agaatcgcca ggcagttttt tcgcgctggc ccgctgccct ataattgcac
      661 acaacggagc aatgcctatg cgctgggact ggcggaatcg gaataagcgg accggcaaac
      721 gcgcatcgcg ctggaacacg gtcgcacgcg gccttgcgct cgttgggttg atcgccctgg
      781 cggcgcactg ggccggacgg tggagtgcat ccccttatgc cagccctgga ctctcgtccg
      841 ccgcagcgca ggaggccgtc aagccggacg accccctggc gaaaggcgac ttgcccgcag
      901 ccggtgatca ggagcgcgga gcgcggatcg tccgcttcga cgtgccgctg ccgatcgacg
      961 gactggtgga ttcgcatatc caaagccgcg tcgagcaggc cctccgcaag ctgccagagg
     1021 gaggcccgcg gccgaccttc gtgttcgaat tccgcccgaa aaacaacacg gccggcgaag
     1081 ggaacgattt cgagcgcgcg ctgtcgctgg cccgctattt gtgcggcgat cggctggctg
     1141 gagtgcgtac cgtcgcctgg ttgccgcggt cggtcaaagg gcacgccgtg ctgccggtgc
     1201 tggcctgcga gcaaatcatc gtgcaaaaag aggccgagct gggagcggcc ggcgccgatg
     1261 agcggggggc gatcgacgct ctcacccgcc gctgctacca ggaaatcgcc gaccgccgca
     1321 agaccgtccc cacggccatc gcccttggct ttctcgacaa ggaactgacg gtttacaagg
     1381 tgaaaacgcc cgacgggctg cggtacgaac tcgacgacga cctgcgcagc ttgcggcagg
     1441 aaggcaaaat cacgagccag gaaacgctgt tccagcccgg cgatccgcac gtgctgagcg
     1501 gcgcgcagct gcgcgagctg ggctttgcct cgcacctggc cgacgaccgg cgcacactgg
     1561 ccgcggctct cgacgttccg atggcggccc tccagcagca attggttccc gaagatggct
     1621 ggaagccact ccgcctcgat ctgaacggcc ccgtgcaccg ccaagcggtc aatttcctgc
     1681 tgcgaacgat cgaagaccac gagcggcggc gcgatttcaa cctgctcgtc gtctgcatcc
     1741 agtcaggcgg cggcgatttg agccagagcg tgcggctggc cgagaagatc gcctcgctcg
     1801 ggccgcagct acacagcgtg gcggttgtcg attcgcaagc ccgcggcgac gctgtgctgg
     1861 tagccgtggc gtgcgacgag ttactggtga cggccgacgc catgctcggc ggtcccggcg
     1921 agcgggccct cagtcgcgaa gaattgaccg acctgcgggg tccattgccg ggcattgccc
     1981 atgccgcgga ccgcgactgg tcgctgccgc tggctctcgt cgattcgcag gtgcaggtgt
     2041 tccgcacgac gcgcacggtg ggaggcgagg tgcggtatct ctcgcccgac gagctggaaa
     2101 cgctgcccga ccgcgaacag tggaatcgcg acgaccagcc gctgccgacg accaacggca
     2161 tcaccggaca gtccgccgtc gaatgggggc tggcccgggc ggtcgtccac aacctggacg
     2221 acgctatgtc gctgttccag atcgaaggcg agctgcccac cgcccggccc aactggg
//
LOCUS       DASUGB010000400         9538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1017806, whole genome shotgun sequence.
ACCESSION   DASUGB010000400 DASUGB010000000
VERSION     DASUGB010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017806"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFV87_07675"
     CDS             <1..217
                     /locus_tag="VFV87_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX5103671.1"
                     /translation="TDGDYVIVKKQKTAQPGQLVVALTPEGESTLKFWFPESNRIRLQ
                     PANKEMQPLYVKDATVQGIVVGVVRNM"
     gene            303..1535
                     /locus_tag="VFV87_07680"
     CDS             303..1535
                     /locus_tag="VFV87_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5103672.1"
                     /translation="MSHASAIVLVVDRLGAGHLGPYGNTWLDTPHFNRLAARSLVCET
                     VLADSPDLAAAYRGWWTGRHVLEAEVASSVPLPARAAASGAATLLVTDDPAVADDPLA
                     AGFAKRVIVETRTPARTCAEVEQTGLFRLFASAIESIERSQTPMLAWIHARGMAGPWD
                     APRAYRERLADPEDPPPPDLIAPPERRLDRHVDPDEVLGLVQAYGGQVQLVDECLGLL
                     LEALERLPRADETLFAVTSPRGYPLGEHGRVGPCDEALYSELLQVPLMIQFPRGEGRL
                     QRTQHIVQPADLFATLADACGFGELAADERDASLLAHIRGDSRRVRNLACATGPNQRA
                     IRTAAWYLREIREHAEPRYELFAKPDDRWEANEVSSRCEEVAALLAAELDRFEQAARA
                     GELAKLASSAEILQDIWR"
     gene            1695..5618
                     /locus_tag="VFV87_07685"
     CDS             1695..5618
                     /locus_tag="VFV87_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103673.1"
                     /translation="MLVCALAAAFSLAVLVGNVAATAAEFDLGLRIAWGGGEGTSWQG
                     TIRLSAGVLADPAPLGLEADDPGSMQVVADTMLRIHPRSPRSYDGCDLRVKAPADAKL
                     LIELAPEGAPPPQPLEIPLAQLLKGVAQFPLDERQNRLLVQRSPGDALRVQFAHEHLV
                     FAPGEKLTLNVEPNPADVTPSTSYLLAASLLYGRSEEQLWNKDLELKTDAGARAALAT
                     VEVPLPLDEGVYDVRLAVYPKRLTSTFVRGKPLLTRKVQLVVVAPVKTQPVAQPEWQS
                     ILEFDPANPKWWERMTRLPSSLRLPTLPAAPVGSGATLTRTHQSRGWVELAPGAWQAY
                     PLTVTTPGRPHVLEIEYPSDWEQTLSISLVEPNAAGEVAPIGLDSGLDVPAPAAGHTP
                     AVQRHKLIFWPNTRSPQVLLVNRRDDRPALYGQFNLLAGPTELPPLAVPPSRLPGRTL
                     AAYYDRPLVVENFSAAEFLDPASNRCLDDWQTFLDAGRRLIETLQYSGHNAVVLTVAS
                     EGSAIYPSSLLEPTPKYDTGMFFESGQDPRRKDVLELLFRLCDRAGINLIPAVQFSSP
                     LPALEALRLAGGSEATGLEPLGPDGRTWLQRGGARRGMGVYYNALDERVQAAMASVVA
                     ELANRYGHHASFAGVSVQLGAESYAVLPDETCSYDDATLAAFAQQAAVELPDACTTSL
                     AARSEFLHGEGEEAWLAWRTTRLSTLYSRMRAEVARHRNSAGLYLATGDLTSGRQVQL
                     AMRPSLPERSNPAFVLRLMGIDLQQLASVGIVAPRPGRIVPNIAAARDFHEHWNQSPE
                     LDAVFRQGGRATALHVLEPAQLPLPEFDAVSPFGAEKTHTWLVSQIPPAGAAYRERFV
                     RSLAALDAPAIFDGGWLLPLGQEAELAPFAKVFRRLPAEAFATLQPAAGAEQSSEVVV
                     RWLTRGGRTYFYAVNAAPWPVEVAIEFASSQPLRVSPYADDRPASVESTGESSLWTCA
                     LQPLDVVGGETETLGVALSGYRVTASADIASTLRDEIRTVRLRCNSLRSPIARDWLAN
                     PSFETPSMAANIPGWVNAAGPGLRVEVDRTGGYESKSSLHVVSTAVGERPAPVVWIRS
                     EPFAAPTTGRLSVVAWIRTRDAARQPVLRMAIEGKLDGRTYYRRANVGASEDGLAVKP
                     LTTEWAPYRFPLADLPQSGLTDLRVGFDLMGEGEVWIDNVQVFDLWFESNERDELIKN
                     IATVDVQAGSGQIFECQRFLTSYWPSFLRRHVPLPGATPGVSSRGGSAAAHSAPAPGA
                     PGHITARPAAPKPAGGTPPPPPAGPKEDSPSMVDRLKSWLPKSWR"
     gene            6263..8338
                     /locus_tag="VFV87_07690"
     CDS             6263..8338
                     /locus_tag="VFV87_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5103674.1"
                     /translation="MATGQTQVNTETVEKTKQQIRGLVGEIAQLSKSDLEAEEYYSLF
                     LQKIVQALYAVGGAIWTLGEGKKPHLAYQINLSQNLLEADTEDADKHFRLLDYIVASN
                     TPQLVPPLSSFGDDRLGGNPTRQLLVVHPLGADNQVEGLIEIFQRADSNPQTQKGYLQ
                     FLKQMCELAAEWFKNRKLRHFSDRHTLWAQADQFSRAVHQSLDVKETCYTVVNEGRRL
                     MGCDRVTVAILRGSHCKVEAVSGQDTIDNRSNVVTLLGKLATRVVKSGEPLWYTGSTE
                     DLPPQIEQAIEDYVDQSYTKSLAVVPLRRPEPTAAAAAAAEPNAVGAESEQAHQGEIV
                     GALIVEQIESEIPREILAPRLDMVYEHSTRAISNALEHHNLFLMPVWRAIGKSRVVVQ
                     GRNLPKTVLITLGVLLLLGLGLAIPTDFDMRAKGTLQPLTKADIFAPSPGEVQGVLAD
                     NGALVKAGDPLVKLRNPNLEIQIRQAEGELNAALESLAAYSQQLISNAQKMSSQEKAK
                     MQADAATLQQKVEAQEQTLELLREREAKLTVRSPIAGQVITWDAKKNLENRPVETGQV
                     LMTVAAADTEYEVELYMPERRMKHLGRARELVQKGNADADLKVTYILMTDPGVSHAGT
                     LIRVHGAAEPNEESGNIVRLHVQPDETIPNPRPGATLTADVHCGKAPLLWNYLHEAWE
                     YLQASPIMF"
     gene            8431..9465
                     /locus_tag="VFV87_07695"
     CDS             8431..9465
                     /locus_tag="VFV87_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5103675.1"
                     /translation="MKTASLQSFAPLVLLALAPLAFGQTPSLATKRAITVEGALVTMI
                     DDVNVPATEIGMLTALHVREGQLVDSETLLADIDNRETIAKQLIGQGELEAAQAQAAS
                     TAEIEVAEKAIQVAQAELDSMRDIRKTNPGAVSETELRKYQFQLERAQAQLKLAVTEQ
                     NIARLNVKTKASQLQAISIELDRRQLKAPFRGEVVEIYKKRGEWTQAGEPVLHLVRMD
                     KVRIKGFVNFSEGVSPDQLEGRPVKIVVFSAGDKQHKTTGKINFASRVIEGVSNYRQF
                     RVSVDVENEKLVDPATGKESWAIQPGTMANLAIDLSPAAPLARPAVTPIKTTPVGGRV
                     EVRKPTTTER"
BASE COUNT         1692 a         3189 c         3115 g         1542 t
ORIGIN      
        1 caccgacggc gattatgtga tcgtcaaaaa gcagaagacc gcgcagccgg gacagctcgt
       61 cgtggccctc acgcccgaag gggaatcgac gctcaaattc tggttccccg aatcgaaccg
      121 catccgcctg cagccggcca acaaggaaat gcagccgctg tacgtcaagg acgccacggt
      181 gcagggcatc gtcgtgggcg tcgtgcggaa tatgtaggtt ccgacggccg cggcggaact
      241 ggacgcgcca tggactcaac tcgcacctcc ggtcccgccc aggcggtcgg gacctacgat
      301 tgatgtctca tgccagtgcg atagtcctcg ttgtcgatcg cctgggggcc gggcatttgg
      361 gtccctacgg caatacgtgg ctcgatacgc cgcacttcaa tcggctggcg gcgcggtcgc
      421 tggtgtgcga aaccgtgctg gccgattcgc cggacctggc cgcggcttat cggggctggt
      481 ggacgggccg gcatgtgctg gaggcggaag ttgcatcgag cgtgccgctt cccgcgcggg
      541 ccgcggcaag tggcgcggcc acgctgctgg tgacggacga tccggcagtt gccgatgatc
      601 ctttggcggc gggatttgcg aagcgtgtca ttgtcgagac gcggacgccg gcgcgcactt
      661 gcgcagaggt cgagcaaacc ggcttgtttc gattgttcgc ttcggcgatt gagtcgattg
      721 agcggagtca aacgcccatg ctggcgtgga tacacgcccg cggaatggcg gggccgtggg
      781 atgctccgcg tgcctatcgc gaacggctcg ccgatccgga agatccgccg ccgccggacc
      841 tgatcgctcc gccggagcgc cggttggacc gccacgtcga tcccgacgaa gtcctcggcc
      901 tggtgcaggc atatggcggc caggtgcagc tcgtcgatga gtgcctggga ttgctgctcg
      961 aagcgctcga gcggctgccg cgggcggatg agacgctttt tgcggtgaca tcgccccgcg
     1021 gctatccgct cggcgagcac gggcgtgtcg gcccgtgcga cgaagcgctc tacagcgagc
     1081 tcttgcaggt cccgctgatg atccagtttc cgcgcggcga aggacgcctg cagcgcacgc
     1141 agcacatcgt gcagccggcc gatctgttcg ccacgctcgc agacgcctgc ggctttggcg
     1201 agctggcagc cgacgagcgc gacgcgagct tgctggcgca cattcgcgga gacagccggc
     1261 gtgtcagaaa tctcgcgtgc gcaaccggcc ccaaccagcg ggccatccgc accgccgcgt
     1321 ggtatctgcg cgaaatccgc gagcacgctg aaccacgcta cgagctgttc gccaagccgg
     1381 acgaccgctg ggaagcgaac gaagtttcgt cgcgttgtga ggaggttgcc gccctgctag
     1441 cagcggagct cgaccgcttt gagcaggcag cccgcgccgg cgagcttgcc aagcttgcct
     1501 cgtctgccga gattctgcag gatatctggc gttagctggc aatggcgtga ttccgctgtg
     1561 gcctgcaagc ggcgggcgtt cttataatct gacgcctccc aagggaccat cattttccag
     1621 cgcccatgac cggacaagga agtccgcgat tcggcccggc gccacgattg cgccggccgg
     1681 caagatgcca atcggtgctg gtctgcgcgc tggccgctgc cttttcgctc gccgtgctcg
     1741 tgggcaacgt cgcggcgacc gctgcagagt tcgatctggg cctgcgaatc gcctggggcg
     1801 gcggcgaagg aacctcctgg caaggaacga tccgcctgag cgccggcgtc ctcgccgatc
     1861 ccgcgccgct cggcctggaa gccgacgatc ccggctcgat gcaggtcgtg gccgatacca
     1921 tgctccgcat tcatccgcgc tcgccgcgca gctacgacgg ctgcgatctg cgcgtcaaag
     1981 cgccggccga tgcgaaattg ctcattgagc tcgcgcccga gggggctccg ccgccgcagc
     2041 cgctggaaat tccgctggcg cagttgctca agggggtcgc ccagtttccg ctcgacgagc
     2101 ggcagaaccg cctgctcgtg cagcgttcgc cgggcgacgc gctgcgcgtg caattcgcgc
     2161 acgagcatct cgtcttcgcg ccgggcgaga agctcacgct caacgtcgag ccgaatccgg
     2221 ccgacgtgac gcccagcacc tcgtatttgc tcgccgcgtc gctgctctat ggccgcagcg
     2281 aggaacagct ttggaacaaa gacctggaac tgaagaccga cgccggcgca cgggccgccc
     2341 ttgccaccgt cgaagtcccg ctgccgctgg acgaaggcgt gtacgacgtg cggctggcgg
     2401 tctatccgaa gcggctgacg agcacattcg tccgcggcaa gccgctgctc acgcggaagg
     2461 tgcagctcgt cgtcgtcgcg cccgtgaaga cgcagccggt ggctcagccc gagtggcaaa
     2521 gcatcctgga gttcgacccg gccaatccca agtggtggga gcgcatgacg cgcctgccgt
     2581 cgtcgctgcg tctgcccaca ctgccggcag cgccggtcgg cagcggcgcg actttaacgc
     2641 ggacgcacca gtcacgaggc tgggtcgagc tcgcgcccgg agcgtggcag gcttatccgc
     2701 tcacggttac gacgcccggc cggccgcacg tgctggaaat cgagtatccc agcgactggg
     2761 agcagacgct gagcatcagc ctggtggagc cgaacgccgc cggtgaggtt gcgcccatcg
     2821 ggctcgactc ggggctcgac gtgccggccc ccgcagcggg ccacacgccc gcggtgcagc
     2881 gacacaagtt gatcttttgg ccgaatacgc ggtcgccgca ggtgctgctg gtcaaccgtc
     2941 gcgacgaccg gccggccctc tatggccaat tcaatctgct cgccggcccc acggagctgc
     3001 cgccgttggc ggtgccgccg tcgcgtctgc cgggccgcac gctggccgcc tattacgacc
     3061 ggccgctggt ggtggagaat ttctccgccg cggagttcct cgatcccgcc agcaatcgct
     3121 gcctcgacga ttggcagacg ttcctcgacg ccggccgaag gctgatcgaa acgctgcaat
     3181 atagcggaca caacgcggtc gtcctcaccg tcgccagcga ggggagcgcg atctacccca
     3241 gcagcctcct ggagccgacg cccaagtacg acaccgggat gttcttcgag agtggccagg
     3301 acccgcgccg caaggacgtg ctggagctgc tgtttcgcct ctgcgaccgg gccgggatca
     3361 acctgattcc cgccgtgcag ttctcctcgc cgctcccggc gcttgaagcg ctgcggctgg
     3421 ccggcggcag cgaggccacg ggcctggagc cgctcggtcc cgatggccgc acctggctgc
     3481 agcgcggcgg agcgcggcgg ggaatgggcg tctattacaa cgctctcgac gagcgggtgc
     3541 aggcggcgat ggcgtcggtc gtcgccgagc tcgccaatcg ctatggacac cacgcgtcgt
     3601 ttgccggcgt gagcgtgcag cttggggccg aatcgtacgc cgtgctgccc gacgagacct
     3661 gcagctacga cgacgccacg ctggccgcct tcgcccagca agccgccgtc gagcttccgg
     3721 atgcctgcac aacgtcgctg gccgcccgca gcgaattcct gcacggggag ggcgaagagg
     3781 cgtggctcgc ctggcggacg acgcgcctga gcacgctcta ttcgcggatg cgggccgaag
     3841 ttgcccgcca tcgcaattcg gcggggcttt atctggcgac tggcgacctc acgagcggcc
     3901 ggcaggtgca actggccatg cggccgtcgc tccccgaaag gagcaatcca gcgttcgtgt
     3961 tgcggctgat gggaattgac ttgcagcaac tcgccagcgt gggaatcgtc gcgccgcggc
     4021 cagggcggat cgtgccgaat atcgccgccg cccgcgattt ccatgagcat tggaaccaat
     4081 cgcccgagct cgacgccgtc ttccgccagg gcggacgcgc gaccgcgctg cacgtgctgg
     4141 agcccgcaca gctaccgctg ccggaatttg acgccgtcag cccgttcggc gcggaaaaga
     4201 cccacacctg gctcgtctcg cagattccgc ccgcgggcgc cgcctatcgc gagcggttcg
     4261 tccgcagcct ggccgcgctc gacgcgccag caatcttcga cggcggctgg ctcttgccac
     4321 tcgggcagga agcggagctg gcccccttcg cgaaggtttt ccggcggctg ccggcggagg
     4381 cgttcgccac gctccagccg gcggccggcg ccgagcaatc gtcggaagtc gtggtccgct
     4441 ggctgacgcg cggcggccgg acgtacttct acgccgtgaa tgccgctccc tggccggtgg
     4501 aagtcgcgat cgaattcgcc tcctctcagc cgcttcgcgt atcgccctat gccgacgacc
     4561 gtcccgcgag tgttgaatcc accggcgaga gcagcctctg gacctgcgcg ctccagccgc
     4621 tggacgtggt cggcggcgaa acggagacgc tgggcgttgc cctgtctggg tatcgagtca
     4681 ccgcgtccgc cgatattgcc agcacgctgc gggacgaaat ccgcacggtc cgcctccgct
     4741 gcaactccct gcgctcgccc atcgcccgcg actggctcgc caatccctcg ttcgaaactc
     4801 cttccatggc ggcgaacatt cccggctggg tcaacgccgc ggggccaggg ttgcgggttg
     4861 aagtcgatcg caccggtggc tacgaatcga aaagctcgct gcacgtcgtc agcacggcgg
     4921 ttggcgagcg gccggcgccg gtcgtctgga ttcgcagcga gccctttgcc gcgccaacga
     4981 cgggccgcct gtcggtcgtc gcctggattc gaacccgcga tgccgcccgc cagccggtgt
     5041 tgcgaatggc gatcgaaggc aagctcgacg gccgcaccta ctaccggcgg gcgaacgtcg
     5101 gcgcgagcga agacggcctt gcggtcaagc cgctcacgac cgagtgggcc ccctaccgct
     5161 ttccgctggc cgatctgccg cagtcggggc tgaccgattt gcgcgtgggc ttcgacctga
     5221 tgggcgaggg agaggtctgg atcgacaacg tgcaggtctt cgacctgtgg ttcgagagta
     5281 acgagcgcga cgagctgatc aagaatatcg ccacggtcga tgtccaggcc ggctcgggac
     5341 agatcttcga atgccagcgg ttcctgacga gctactggcc cagcttcctg cggcggcacg
     5401 tgcccttgcc gggcgctact cccggcgtga gcagtcgcgg cgggagcgcg gcggcgcact
     5461 ccgctccggc tccaggcgcc ccgggccaca tcacggccag acctgccgcg ccgaagcccg
     5521 caggcgggac tccgcctccg ccaccggcgg ggccgaagga agactcccct tccatggtcg
     5581 atcgtctcaa gagctggctg cccaagtcgt ggcgctgatt gaatcttgca tctggcttcg
     5641 taggacaggt ttcaatccct tgctgtagga caggttttca acctgtccct ttcgcggacg
     5701 ggctggaaac ccgtcctaag cccggtgcaa gcaccggggc gaaatgttcg cgaatgcaac
     5761 gtcctgctgt cagaacgaaa cgatcgtagg cactactgga agtctgcccc acgggcttga
     5821 agaattgaca aggccagcct gccgctgctg ccgcggatat cccggcgctg cgatcgttac
     5881 aaccgtcgcc gcctacgctt acgccgccaa atcggccacc cgccaacctt caggtatggc
     5941 gtttgcgtca ggggtcggtt ccgtcccgct ggctgccaga acggcgtggc gcgatttcgt
     6001 tcgttccgct cctcgtcaag gccgtcgcac gtcatccatt cgatacatct gctcattctg
     6061 gaccgttctt ttgttaattg acgtaaacca aaacgttgta aggttttgac gcgacgttta
     6121 gattggataa ttagtcagta gcatcatgca ccagcacgcc gcactggcgg cgaaacgtcc
     6181 gggcgggcct cggccctccg tgcggctgga gaagctgctg ccttgtcgcg gcccccgcac
     6241 ctgaaagccg agaaccgccc gcatggccac cggacaaacg caggtcaaca ccgagaccgt
     6301 cgaaaagacc aagcagcaga ttcgcggcct cgtgggcgaa atcgcgcagc tttccaagag
     6361 cgacctggag gccgaggagt actactcgct cttcctgcag aagatcgtgc aggcgctgta
     6421 cgccgtcggc ggcgcgatct ggacgctggg cgaaggcaag aagccgcatc tcgcctatca
     6481 gattaacctc agccagaacc tgctcgaagc ggacaccgaa gacgccgaca agcacttccg
     6541 cctgctcgat tacatcgtcg ccagcaacac tcctcagctc gttccaccgc tctcgtcgtt
     6601 cggcgacgac cgcctgggtg gaaatcccac acggcaactg ctcgtcgtcc atccactcgg
     6661 ggccgacaac caggtcgaag ggctgatcga aatcttccag cgggccgaca gcaacccgca
     6721 aacccaaaag ggctatctgc agttcctcaa gcagatgtgc gagctggcgg cggagtggtt
     6781 caagaaccgc aagctgcggc atttcagcga ccgccacacg ctgtgggccc aggccgacca
     6841 gttctcgcgg gccgtacacc aaagcctcga tgtcaaagaa acgtgctaca ccgtcgtcaa
     6901 cgagggccgc cgcctgatgg gctgcgaccg cgtgacggtc gccatcctcc gcggcagcca
     6961 ttgcaaggtc gaagccgtca gcggccaaga cacgatcgac aaccgcagca acgtcgtcac
     7021 gctgctcggc aagctggcga cgcgcgtcgt caaatcgggc gagccgctct ggtacaccgg
     7081 ctcgaccgag gacttgccgc cgcagatcga gcaggcgatc gaggattatg tcgatcagtc
     7141 gtacacgaaa tcactcgccg tcgtgccgct ccgcagaccc gagccgacgg ccgccgcggc
     7201 cgcagccgcc gagccaaacg cggtgggagc cgaatccgag caggcccacc aaggtgaaat
     7261 cgtgggggcc ttgatcgtcg agcagatcga gagcgaaatc ccccgcgaaa tcctcgcgcc
     7321 gcggctggac atggtgtatg agcacagcac gcgggccatt tccaatgccc tggagcacca
     7381 caacctgttc ctgatgcccg tctggcgggc gatcggcaag tcgcgggtcg tcgtccaggg
     7441 gcggaacctg cccaagaccg tgttgattac gctcggagtc ctgctcttgc tgggtttggg
     7501 ccttgccatt cccaccgact tcgatatgcg cgccaaaggc accctccagc cgctgaccaa
     7561 ggcggacatt tttgcgccga gccccggcga ggttcaaggc gtgctggcgg acaacggcgc
     7621 gctggtgaaa gccggcgatc cgctggtgaa acttcgcaat cccaacctgg aaattcagat
     7681 tcgccaggcc gaaggcgagc tgaatgccgc gctggagtcg ctggcggcgt acagccagca
     7741 gctcatcagc aacgcccaaa agatgagctc ccaggagaaa gccaagatgc aggcggacgc
     7801 ggccacgctg cagcagaagg tcgaagctca ggagcagacc ttggaattgc tccgcgagcg
     7861 cgaggcgaag ctgacagtcc gcagcccgat cgcgggtcag gtgataacgt gggacgccaa
     7921 gaagaacctg gaaaaccgcc ccgtcgagac gggacaggtc ttaatgacgg ttgccgccgc
     7981 ggataccgaa tacgaagtcg agctgtacat gcccgagcgg cggatgaagc acctggggcg
     8041 cgcccgcgag ctggtccaga agggcaacgc cgacgcggac ctgaaggtca cctacatcct
     8101 gatgaccgac ccgggcgtct cgcacgccgg aaccctcatc cgcgtccacg gcgccgcgga
     8161 gcccaacgag gaaagcggca atatcgtgcg tttgcacgtg cagccggacg agacgattcc
     8221 caatcctcgt cccggcgcga cgctgaccgc ggacgtccat tgcggcaagg cgccgctgct
     8281 gtggaactac ctgcacgaag cgtgggaata cctgcaggcc agtcccatca tgttctagtc
     8341 ctcccactct ttccttcgtc tgtagccgca ctcgccagag tgcgggtgac ataacggcca
     8401 atcgtgatga gcctctcccg aggaatcgca atgaaaaccg cctcgctcca atcattcgct
     8461 ccgctggtgc tgctcgccct cgcgccgctg gccttcggcc agacgccgag cctcgcgacg
     8521 aagcgcgcaa tcacggtcga aggcgcactg gtcacgatga tcgacgacgt caacgtgccg
     8581 gcgacggaga tcggaatgct gacggccctc cacgtccgcg aagggcagct cgttgattcg
     8641 gagacgctgc tggccgacat cgacaaccgc gagacgatcg ccaagcagct catcggccag
     8701 ggcgaattgg aagcggcgca ggcccaggcc gcgagcaccg ccgaaatcga ggtggccgaa
     8761 aaggcgattc aggtcgctca ggcggaactc gactcgatgc gggacattcg caagacgaat
     8821 ccgggcgccg tgagcgagac ggagctgcga aagtaccagt ttcagctcga gcgggcccaa
     8881 gcgcagctca agctggcggt gaccgagcag aacatcgccc gcctcaacgt gaaaacgaag
     8941 gcctcgcagc tccaggcgat cagcatcgag ctcgaccgcc ggcagctcaa ggcgccgttc
     9001 cggggagaag tggtcgagat ttacaagaag cgcggcgaat ggacccaggc cggcgagccg
     9061 gtcctgcacc tggtgcggat ggataaggtg cggatcaagg gctttgtcaa tttctcggag
     9121 ggcgtctctc ccgaccaact ggaaggcagg ccggtcaaga tcgtcgtctt ttccgccggc
     9181 gacaagcagc acaagaccac aggcaaaatc aacttcgcca gccgcgtgat tgaaggcgtg
     9241 agcaattacc gccagttccg cgtgtcggtg gatgtggaga acgagaagct cgtcgatccg
     9301 gcgacgggga aggaaagctg ggcgatccag ccgggcacga tggccaatct ggcgatcgac
     9361 ctgtcgccag cggcgccgct ggcccggccg gcggtgacgc cgatcaagac gacccccgtt
     9421 ggcggccggg ttgaagtccg caagccgacg accacggagc gttagcggaa actcggtccc
     9481 cagggtcctc cctgggaccg cacgtccgcg aggctcctgc ctgggaacgc acttcccg
//
LOCUS       DASUGB010000401         6276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_879111, whole genome shotgun sequence.
ACCESSION   DASUGB010000401 DASUGB010000000
VERSION     DASUGB010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879111"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFV87_07700"
     CDS             complement(<1..917)
                     /locus_tag="VFV87_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103676.1"
                     /translation="MFDLFTTSTNCQGTSRRAFLRVGSLSLLGLSLPQVLAAKARAAG
                     KPAKDINCILLWTEGGMSNVDTLDMKPDAPIEYRGQFLPVRSNVPGIDVCEHLPGMAK
                     AMDKVCLVKSIAHSESGDHVAATHYMLTGYPQRPDVTGNPVGSTVYPAIGSLVARQKG
                     WQNGLPPNISFGRESYGGGGYMGSAYNPLIIPGDPSHKDFRVDDVTLSSAIGPERAAR
                     RRTMLQKLDAWQAAAETARGPLADRGQFYQQAYDLLTSPAAKKAFALSEEPDSVRNRY
                     GRTREGQATLLSRRLIEAGVRFVSVITTG"
     gene            complement(1001..3184)
                     /locus_tag="VFV87_07705"
     CDS             complement(1001..3184)
                     /locus_tag="VFV87_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5103677.1"
                     /translation="MRRLAATLLHGLVALTVCAWATELGQAEDAGQQPVSFRRDVIPA
                     LTKAGCNAGACHGSFQGRGGFRLSLLGFDPEADYETIAVDSRGRRVLPTSPASSLLLL
                     KATGAVPHGGGVRLTADQPAYAILHDYIAAGLPAPSADEPRIAGLDVSPLDAVLQPGE
                     STHLQVTARWSDGSTRDVTEWALFDARDATATEVTREGQLTSLRPGKASASVRYLGQV
                     ASVAITTPFGPPPPIEFMPANYIDELVLVEWQRMGVRPTALCSDGEFLRRAHLDLIGT
                     LPTADEVRTFLASTDPDKRKKLIDALLERPEYGDYWSLKWGDLLRVHRRYVGEKGLGS
                     FGGWLRQSIRENKPLDQLAKELLTAQGNLFSNGPVAYFLVDTKPEELAETTAQLFLGV
                     RLQCARCHHHPMEVWGQDDYYGLAAFFTRLEVRENGDKGRFGGMHIVRPVAKAMKDLQ
                     VASPPRLFGQTTPLDPAATPDVRQQLAEWLTAKDNPFFARNFANRYWAYLLGRGLVQP
                     IDDMRATNPASNPALLDALSKDFAEHGYDAKHLLRTIANSRVYQLATDLAPEIDKDGA
                     LFTHHVPRRMPAEVLLDAINQVTGHVETFPGQPPGTRAISLPDPAVDSDFLTMFGRPL
                     RNNPCECARESSPDLLQALHLINNQELQSKIAGDKGRLAGLIAGQKTDDQMIEELYLS
                     TYARLPTAGESAAIRELAADVTSRKEMFEDLLWTLLNSPEFTFNH"
     gene            3403..4731
                     /locus_tag="VFV87_07710"
     CDS             3403..4731
                     /locus_tag="VFV87_07710"
                     /inference="COORDINATES: protein motif:HMM:NF041199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trx7/PDZ domain-containing (seleno)protein"
                     /protein_id="HEX5103678.1"
                     /translation="MSRSPAVALACLVLFPLVAAAQTREQKVRADKEKVEAAGFWIYN
                     DLPKAFAEAKQTGQPLVVALRCIPCVECVKLDDELVDQDERLQAALEKFVRVRVVSAN
                     GLDLSLFQFDTDQSFAVFMLNADGTIYGRYGTRSDHSHWADDVSVEGLAKALEGALEL
                     HAEYPANKAVLAAKRGPKPDFAAPENYPTLKGKYGSQLNYEGNVVQSCIHCHQIGDAQ
                     RQFYRDKETALPDRVLFPYPHPKSLGLILDPKERATVLRVEPGSLAAKAGFEAGDQIR
                     SLSSQPLLSMADVQWVLHHVQAEGGSVPAIAERRGKAVQLTLSLPAGWRGMDDISWRA
                     SSWELRRIGLGGLLLKDVSAELREKQNLPAEGLALRVEHLGQYPPHNVAQQAGFRKDD
                     VLIAFDGRTDLARETDLLHYTLNDKKPGSSVPVTVLREGKKIDLTLPIGK"
     gene            complement(4745..6001)
                     /locus_tag="VFV87_07715"
     CDS             complement(4745..6001)
                     /locus_tag="VFV87_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX5103679.1"
                     /translation="MISLSHAALASAADFPPPDALKSNTSLPDPLVMRDGTKVESRED
                     WEAKRKPELKALFQHYMYGLIPPKPVRQEFQTGEPRDCLEGKATLREITIHSLEPRTG
                     HPLHVLLIAPKGVERPPVFVGMNFCGNHTLLDDPKVPLPTGWVRNSCVGALNERATDK
                     GRGSQKDVWNADLIVSRGYALATFYSGDVDPDTPDLADGIGPTYFKGTSESPAGDDPG
                     TIALWSWGFRRVVDYLTEGGGKELVDPQRIAVVGHSRNGKTALLAGAFDERIALIIPH
                     QAGCGGTAPSRTDDPKAESVKRINTSFPHWFCENFVLFNDDVARLPFDQHCLLALCAP
                     RPVLYSNAQDDQWANPNGQFEMLRAAEPVYKLYGVEGLAPDARPEIGKLVKSRLGYFL
                     REGKHSMSREDWEVFLDYVDANLKGR"
     gene            complement(6119..6189)
                     /locus_tag="VFV87_07720"
     tRNA            complement(6119..6189)
                     /locus_tag="VFV87_07720"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6155..6157),aa:Gln,seq:ctg)
BASE COUNT         1104 a         2040 c         2015 g         1117 t
ORIGIN      
        1 cacccggtgg tgatgacgct gacgaaacgc acccccgcct cgatcagccg ccgcgatagg
       61 agcgtcgcct ggccctcgcg cgtccggccg tagcgattgc ggacggagtc cggctcttca
      121 gaaagtgcga acgccttctt cgccgcgggc gaggtgagca ggtcgtaggc ctgctgatag
      181 aactggccgc ggtcggctaa gggaccgcgg gccgtttcgg ctgccgcttg ccaggcgtcg
      241 agcttttgga gcatcgtccg acgccgggcc gctcgctcgg ggccgattgc cgatgaaagc
      301 gtcacgtcat cgacccgaaa gtccttgtgc gacggatcgc ccgggatgat tagcgggttg
      361 taagccgagc ccatgtaccc gccgccgccg tacgactcgc ggccaaacga aatgttcggc
      421 ggcaggccgt tctgccaccc cttctgccgg gcgacgagcg agccgatcgc gggatagacc
      481 gtcgatccga ccgggttacc ggtcacgtcg ggccgctgcg gatagccggt gagcatgtag
      541 tgtgttgccg cgacgtggtc gccgctctcg ctgtgggcaa tcgactttac gaggcaaacc
      601 ttgtccatcg ccttggccat gcccggcaga tgctcgcaaa cgtcgatccc cggcacgttc
      661 gagcggaccg gcaggaactg gccgcggtac tcgatcggcg cgtccggctt catgtccagc
      721 gtatcgacgt tgctcatccc gccttcggtc caaagcagga tgcagttgat gtccttggcc
      781 ggcttccccg ccgcgcgggc cttcgccgcc aagacctgcg gcagcgagag gcccaatagc
      841 gacaggctgc cgacgcggag aaacgcccgg cgcgatgtgc cctggcagtt ggtggaggtg
      901 gtgaagaggt cgaacatcgc agatcctcgc aatcatgttt aacgcgaagc cgaaggcgcc
      961 gcgtcacgtc cggctcgcct tcggctcgcc gttaaacaag tcagtgattg aatgtgaact
     1021 ccgggctatt caatagtgtc cacagtaagt cctcgaacat ctccttgcgg ctcgtcacgt
     1081 ccgcggccag ttcgcgaatc gcggcagatt cgccggccgt tggcagccgg gcgtaggttg
     1141 acagatagag ttcctcgatc atctggtcat cggttttctg gccggcaatc agtccggcca
     1201 gccggccttt atcgccggca atcttgctct gcaattcctg attgttgatc aagtgcagcg
     1261 cctgcagcag atcgggcgac gattcgcggg cgcattcgca cggattgttc cgcagcggcc
     1321 ggccgaacat cgtcagaaag tcgctatcca ccgccggatc gggcagcgag atggcccgcg
     1381 tgcccggcgg ctggccggga aaggtttcca cgtgtccggt gacctggttg atcgcgtcca
     1441 gcagcacttc agccggcatc cgccgcggaa cgtggtgcgt gaacaaggcg ccgtccttgt
     1501 cgatttcggg cgcaaggtcg gtggcgagct gatacacgcg cgaattggcg atcgtccgca
     1561 agaggtgttt cgcgtcgtag ccatgttcgg cgaagtcttt cgacagtgca tcgagcagcg
     1621 ccgggttgct cgccgggttg gtcgcccgca tgtcgtcgat tggctggacg aggccgcggc
     1681 ccagcagata agcccagtag cggttagcga aattgcgggc gaagaacggg ttgtccttcg
     1741 cggtcagcca ctcggcaagc tgctggcgga cgtcgggcgt cgctgccgga tcgagcgggg
     1801 ttgtttggcc gaacaatcgc ggcggcgagg cgacctgcaa gtctttcata gcctttgcga
     1861 ccggccgcac gatgtgcatc ccgccgaagc gccccttgtc gccgttttct cggacctcca
     1921 gccgcgtgaa gaacgccgcc agaccgtagt agtcgtcctg cccccagacc tccatcggat
     1981 ggtggtgaca gcgggcacac tgtaagcgca caccgaggaa gagctgcgcc gtcgtctcgg
     2041 ccaattcctc cggcttcgta tcgacgagga aataggcgac cgggccgttc gagaacagat
     2101 tgccctgcgc cgtcagcaat tccttcgcaa gctgatcgag cggtttgttc tcgcggatcg
     2161 actgccgcag ccagccgccg aagctgccca gccccttttc gcccacgtag cggcgatgga
     2221 cgcgcagtaa atcgccccat ttgagcgacc agtagtcgcc atactcgggc cgctcgagca
     2281 gcgcgtcgat cagcttcttc cgcttgtcgg gatcggtcga ggcgaggaac gtgcggacct
     2341 cgtcggcggt cggcagcgtg ccgatcaagt cgaggtgtgc gcggcgaagg aattcgccgt
     2401 cggaacaaag cgccgttggc cgaacgccca tccgttgcca ctcgaccaga accagttcgt
     2461 cgatgtaatt cgccggcatg aactcgattg gcggcggcgg gccgaagggc gtggtgatcg
     2521 caaccgacgc gacctgcccg agataacgca cgctggcgga cgccttgccc gggcgaaggc
     2581 tcgtaagctg cccttcgcgc gtcacctcgg tcgcggtggc gtcgcgagcg tcgaacagcg
     2641 cccactccgt cacgtcgcgc gtgctgccgt ccgaccagcg ggccgtcact tgaagatgcg
     2701 tcgattcgcc cggctgcaaa acggcatcca gcggcgagac gtccaggccg gcaatccgcg
     2761 gctcatcggc ggacggggct ggcaggccgg cggcgatgta gtcgtgcaag atcgcgtacg
     2821 caggctgatc ggccgttagc cgcacgccgc cgccgtgcgg caccgcgcct gtcgccttga
     2881 gcagcagcag gctgctggcg ggactggtcg gcagcacccg ccggccgcgg ctatcgacgg
     2941 caatcgtctc gtagtcagcc tccggatcga atcccagcag cgacagccga aacccgcccc
     3001 gcccctgaaa cgacccgtgg catgctcccg cgttgcagcc ggccttggtc agggcgggaa
     3061 tgacgtcacg gcggaagctc acgggctgct gcccggcgtc ttcggcctgt ccaagttccg
     3121 tggcccaagc gcagacggtg agcgcaacaa ggccgtggag tagtgtggct gcgagacgcc
     3181 gcatgaatcg gccctcggca atcaggcggg aaacgggtcg gccattcaac cgaccggcgg
     3241 gagtgcgcgt caccgcacac ttgttctagt gtccgaagaa tcgtccgtga acgcaagcag
     3301 attttggggc catgtcgagc cgattgcgaa cgtttgacac ttcgcctgcg gcctgtcatg
     3361 atctggctgg cttgttcccg cgaggaattc gaggcttcca ccatgtcccg ctcacctgcc
     3421 gttgcgctgg cctgtttggt cctgttcccg ctggttgccg ccgcccagac tcgcgagcag
     3481 aaagttcggg cggataagga gaaggtcgag gcggcggggt tctggatcta caacgacctg
     3541 ccgaaggctt tcgccgaggc gaagcaaacg ggccagccgc tggtggtggc cttgcggtgc
     3601 attccgtgcg tggagtgcgt caagctcgac gacgagctgg tcgatcagga cgagcggctg
     3661 caggcggcgc tggagaagtt cgtgcgcgtc cgcgtcgttt ctgccaacgg cctcgatctc
     3721 tcgctcttcc agttcgatac ggaccaatcg ttcgccgtct ttatgctgaa cgccgacggc
     3781 acgatctacg gacgctatgg cacgcggtcg gatcattcgc actgggcgga cgatgtgtcg
     3841 gtcgaaggtt tggcgaaggc gctggaagga gcgctcgaat tgcacgccga atatcccgcg
     3901 aacaaagcgg tgctggccgc caagcgcggg cccaagcccg actttgccgc gcccgagaac
     3961 tatccaacgc tcaagggcaa gtacggcagc caactgaact acgagggcaa cgtcgtccaa
     4021 agctgcatcc actgccacca gatcggcgac gcccagcggc agttctatcg cgacaaggaa
     4081 acggcactgc ccgatagggt gctatttcct tatccacatc ccaagagcct cggcctgatc
     4141 ctcgatccca aagagcgggc gacggtgctg cgcgtggagc ccggctcgct cgccgcgaaa
     4201 gccggctttg aggccggaga tcaaatccga tcgctctcca gccagccgct gctctcgatg
     4261 gccgatgtgc agtgggtgct gcaccacgtg caggcggaag gcgggtcggt tcctgcgatc
     4321 gccgagcgcc gcggcaaagc ggtgcagctc acgctcagcc tccccgccgg ctggcgtggg
     4381 atggacgaca tttcctggcg cgcttcgagc tgggagctgc ggcggatcgg cctgggcggg
     4441 ttgttgctga aagatgtgtc tgccgagctg cgcgaaaagc agaatctgcc ggccgagggg
     4501 ctggccctgc gcgtcgaaca cctcggccag tacccgccgc acaacgtcgc ccagcaagcc
     4561 ggcttccgca aggacgacgt cctgatcgcc ttcgacggcc gcacggacct cgcgcgcgaa
     4621 accgacctcc tgcactacac gctcaacgat aaaaagcccg gcagcagcgt gccggtcacg
     4681 gtgctccgtg aggggaagaa gatcgacctg acgttgccga tcggcaagta aaccctgttt
     4741 ggttctacct ccccttcagg ttcgcatcca catagtcgag gaacacttcc caatcctcgc
     4801 ggctcatgct gtgcttgcct tcgcggagga agtagcccag gcggctcttg acgagcttgc
     4861 cgatttcggg cctggcgtcc ggagccaagc cctccacgcc gtagagcttg taaacgggct
     4921 cggcggcgcg gagcatctcg aattggccgt taggattggc ccactggtca tcctgggcgt
     4981 tcgaatagag caccggccgc ggggcgcaca gcgccagcag gcagtgctga tcgaacggca
     5041 gacgcgccac gtcgtcgttg aacaacacaa agttctcgca aaaccagtgc gggaagctcg
     5101 tgttgatccg cttgaccgac tcggctttcg ggtcatccgt gcggctgggc gctgtgccgc
     5161 cgcagccggc ctggtgcggg atgatgaggg cgatccgctc gtcgaaggcc ccggccaaca
     5221 gcgccgtctt gccgttgcgc gagtggccga cgacggcgat ccgctgggga tcgacaagtt
     5281 cctttccgcc gccttcggtc aggtaatcga cgacccgccg aaacccccaa gaccacaggg
     5341 cgatcgtccc gggatcgtcg ccggccggac tctcgctcgt gcccttgaaa tacgtcgggc
     5401 caatgccgtc ggccaggtcc ggcgtgtccg ggtccacgtc gccgctgtag aacgtcgcca
     5461 gggcatagcc gcgactgacg atcagatcgg cgttccaaac gtccttctgg ctgccgcggc
     5521 ccttgtccgt cgcccgttcg ttcagggcgc ccacgcagct attgcggacc caaccggtcg
     5581 gcagcggcac cttggggtcg tccagcagcg tgtgattgcc gcagaagttc atccccacga
     5641 agaccggcgg ccgttccaca cccttgggcg cgatcagcag cacgtgcagc ggatgcccgg
     5701 tccgcggctc caggctgtgg atcgtgatct cgcgcagcgt cgccttcccc tccaggcaat
     5761 cccgcggctc gcccgtctga aactcctgcc gcaccggctt cggtggaatc aagccataca
     5821 tatagtgctg aaacagcgcc ttcagctccg gcttccgttt cgcctcccag tcctcacggc
     5881 tctcgacttt cgtcccatcc cgcatcacca gcggatcggg caggctcgtg ttcgacttca
     5941 gagcatccgg cggcggaaaa tcggctgcag aagcaagtgc cgcatggctg agtgaaatca
     6001 tgcaaacaag gagccaggcg ttgcgaacac caggcacaag ggagaagggg actcgcatgc
     6061 aacagactcc gcggcactcc agaagcacga cccgaacgca cagcgcgaag caagaaagtt
     6121 gcggagtaag gatttgaacc ttaactaact ggttcagagc cagtcgtgct accgttacac
     6181 cactccgcag aatctgcctt cattcgggtt ccctactcct tgcgccacac ctctcgttcg
     6241 caagctcggt cggaaaaccc ctggcctacg gctcag
//
LOCUS       DASUGB010000402         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1573908, whole genome shotgun sequence.
ACCESSION   DASUGB010000402 DASUGB010000000
VERSION     DASUGB010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573908"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1357
                     /gene="floA"
                     /locus_tag="VFV87_07725"
     CDS             407..1357
                     /gene="floA"
                     /locus_tag="VFV87_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650218.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEX5103680.1"
                     /translation="MSGMFVLIVGVAIIIGLFLLIAALAFAAYGKQWFQAYMSSADVS
                     ILSLVGMGFRQVNSRVIVQAKIMASQAGLDINRRYGISTQRLEAHYLAGGNVTNVILA
                     IIAAHRAGIPLDFDRAAAIDLAGRDVLDAVRTSVMPKIIDCPDPRGGKAILSAVASDG
                     IEMLVKARVTVRTNLSQLIGGATEETIIARVGQGIVTSIGSAKTHKEVLANPNQISRI
                     LLERGLDAQTAFEIVSIDIATVQVGQNIGARLQADAAEADTRVARAMAEQRRAEAIAL
                     EQEMKSKVADNRSRLVEAEAQVPLAMAEAFRKGNLNGNAA"
     gene            1474..3156
                     /locus_tag="VFV87_07730"
     CDS             1474..3156
                     /locus_tag="VFV87_07730"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5103681.1"
                     /translation="MPTPLLLLAVFSADPTAEQIDLLKTFREEFVAITPGEGKFPRTF
                     PFGANPRMEISRPFSIARYEVPQNLWEIVMGSNPSKWKGERNSVEMLSFDEAEQFCKK
                     ATEMMQVAGLIEKDQVIRLPYEREWEYACRAGTTTNYSFGDEADNLGDYAWFTGNAAG
                     NDPPVGAKKANPWGLYDVHGYLWEWTDDEFGEAGGDSGSRMIRGGSWKDKTEDLASSA
                     KKLVKKNVRDDAIGLRCVLAFVAAPLGDGPTKNFTPTAQAKVVPADSKLEKLWAEGEF
                     TEGPALAPDGSILFSDIGTTIYRFDPKTGQTAVFRKPSGRSNGLKFNQKGELVACEGA
                     NTGGNRRISITAGIDGAKDGTVKTLAGSYDGKRFNSPNDLAIGSRGQVYFTDPRYVGD
                     DPRDLDFETVFLVGTDGSVKVATRDIQKPNGIIVTPDDKTLYVSDNNPGGNRHFVRFA
                     IQADGTLGGKKVLHDFGTGRGIDGMTLDRDGNIFATAGTGDKAGIYVFTAEGEHLAFI
                     PTPGDPTNCCFGGSADSGTLYITCASSKEADTKYGLYRIKLNAKGFAVVKLK"
     gene            complement(3198..4016)
                     /locus_tag="VFV87_07735"
     CDS             complement(3198..4016)
                     /locus_tag="VFV87_07735"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5103682.1"
                     /translation="MAADASNPGDSAGPSAAHESVLRRSPRWLAAGYGATTDLLFPPR
                     CASCGQECRSRDGTPLLCASCLAELQPSPLPACRRCAMPCPAADADGKDCYDCRGRRL
                     LFEEVRALGVYQGPLRDVVLRIKHYHHEPLAAALGQLLAERMQQQPLFQPPDLIAPVP
                     MHWLQRMWRGTNAADTLARSLARALGTRISGGLLVCRRMLRRQSRLLPEERRANVRGA
                     FRVSRLSNVTGARVLVIDDVMTTGATAHEAARTLLAAGAASVNIAVVARGTGNF"
     gene            4186..>4495
                     /locus_tag="VFV87_07740"
     CDS             4186..>4495
                     /locus_tag="VFV87_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX5103683.1"
                     /translation="MPKIIDRALVLLFLKSLLVCFVSLAGLSFIVDLANNAEEFLGYV
                     KQHGPLVLLDYYGPRSLLFFDRTAGLLALVSTIFAITLMQRSNEFTALMAAGIGPARI
                     I"
BASE COUNT          866 a         1491 c         1383 g          755 t
ORIGIN      
        1 ttctaacccc gccgaatcac ccgatcgcag atctcctgca aaattaccca tcttcaccaa
       61 attgccaaat agcgatctcg gagcccgcct ttccacagcc ggcctcccga tactgtatag
      121 atgcgcagaa ggccccaatt atttcccacc ccggaagatt tttcccgcgg gcccgcccgc
      181 acgccgaacg cgaccaactc ccgctgctat atttcgcccc cgccatccgc tatcatttgc
      241 agcgactggc ccgctgcgta agcgagggtc ctcacgcgct tacgcttcgg gtgacagttc
      301 gtgacgctcg caacagcagg cagcttctag cggagtggca accatggcgc ttgtgggatt
      361 tcttctgctg gctcagcagc cgggcgaggg cgagggattc ggctggatga gcggcatgtt
      421 cgtcctgatt gtcggcgtgg cgatcattat cggcctcttt ttgctcatcg ccgcgctcgc
      481 cttcgctgcc tacgggaaac aatggttcca ggcctacatg tccagcgcgg acgtgagcat
      541 tctcagcctc gtcggcatgg gctttcggca ggtcaattcc cgcgtcatcg tgcaggccaa
      601 gatcatggcc tcccaggcgg gcctcgatat caaccgccgc tacggcatta gcacccagcg
      661 cctggaggcg cattacctgg cgggcggcaa cgtgacgaac gtcatcctgg cgatcatcgc
      721 ggcccaccgg gccggcattc cgctcgattt cgaccgggcc gcggcgatcg acctcgccgg
      781 ccgcgatgtg ctcgacgccg tccgcaccag cgtcatgccc aagatcatcg actgccccga
      841 cccgcgcggc ggcaaggcga tcctcagcgc cgtggccagc gacgggatcg aaatgctcgt
      901 caaggcccgc gtgaccgttc gcaccaacct gtcgcaactc attggcgggg cgaccgaaga
      961 aaccatcatc gcccgcgtcg gccagggcat cgtcacctcg atcggctccg ccaagacgca
     1021 caaggaggtg ctcgccaacc cgaaccagat ttcccgcatc ctgctggagc gcggcctcga
     1081 tgcccagacg gcgttcgaga tcgtctcgat cgacatcgcc accgtgcagg tcggccagaa
     1141 catcggcgcc cgcctgcagg ccgacgccgc cgaagccgac acccgcgtag cccgggccat
     1201 ggccgagcag cggcgggccg aagccatcgc cctggagcag gaaatgaagt ccaaagtcgc
     1261 cgacaaccgc agccgcctgg tcgaagccga ggcccaggtc cccctggcga tggccgaggc
     1321 cttccgcaaa ggcaacctca acggcaacgc cgcctaagtg gggcaggctt ccagcctgcc
     1381 actattcgac ttctttggcc ggacaatatg aacccgcccg cagtcatcct tctcggcagg
     1441 caggatgcct gccccacgta gccgagatcc accatgccca cccccctcct gctgctcgca
     1501 gtgttctccg ccgatcccac cgccgagcag atcgacctcc tcaaaacctt ccgcgaggag
     1561 ttcgtcgcga tcacgccggg cgaaggcaaa ttcccccgca cttttccatt cggtgccaac
     1621 cccaggatgg aaatcagcag gccgttctcc atcgcacgtt atgaagtgcc gcagaacttg
     1681 tgggaaatcg tcatgggaag caatccgagc aaatggaaag gagagcgcaa ttccgttgaa
     1741 atgctctcgt tcgacgaagc tgagcaattc tgcaagaagg cgactgagat gatgcaggtc
     1801 gcaggcttga ttgagaaaga tcaggtcatc cgcttgccgt acgaaaggga gtgggagtac
     1861 gcgtgcaggg ctgggactac aaccaactat tcctttggcg acgaggccga caatcttggc
     1921 gactacgcct ggttcacggg aaatgcagcg gggaacgacc ctccggtcgg cgcgaagaaa
     1981 gcgaaccctt gggggctgta cgacgtccac ggttacctct gggaatggac tgacgacgag
     2041 tttggtgagg ccgggggtga ttcagggtcg cgaatgattc gcggcggtag ctggaaggac
     2101 aagacagaag acctggccag ctctgcaaaa aagcttgtga agaagaacgt tcgcgacgac
     2161 gcgataggtc ttcggtgtgt cctggcattc gttgcggcgc cacttggcga tggccccacg
     2221 aaaaacttca ctcccaccgc tcaggccaaa gtcgtccccg ccgactcgaa gctcgaaaag
     2281 ctctgggcgg aaggcgaatt caccgaaggc ccggccctcg cgccggacgg ctcgattctg
     2341 ttttccgata tcggcacgac gatctaccgc tttgatccca agaccggtca gactgccgtc
     2401 ttccgcaagc ccagcggccg cagcaacggc ctgaagttca accagaaagg cgagttggtt
     2461 gcctgcgaag gggcgaatac cggcggcaac cgacgaatct cgatcacggc gggaatcgac
     2521 ggggccaagg atggaacggt caagacgctg gccggcagtt acgacggcaa acgcttcaac
     2581 agccccaacg acctggcgat cggcagccgg gggcaggtct atttcaccga cccgcggtac
     2641 gtcggcgatg acccgcgcga tctcgatttc gagacggtct tcctggtcgg cacggacggc
     2701 tcggttaagg tcgccacgcg cgatattcag aagcccaacg ggatcatcgt cacgccggac
     2761 gacaagacgc tctacgtctc cgacaataac cccggcggca atcgtcactt cgtgcgcttc
     2821 gcgatccagg ccgacggcac gctgggcggc aagaaggtgc tgcacgactt cggcacgggc
     2881 cgcggaatcg acgggatgac gctcgaccgg gacggcaaca tctttgccac cgccggcacg
     2941 ggcgacaaag cgggcatcta cgtcttcacc gcggaaggcg agcacctcgc cttcatcccc
     3001 acgcccggcg accccaccaa ttgctgcttc ggcggcagcg cggacagcgg cacgctctac
     3061 atcacttgcg ccagctccaa agaggccgat acgaaatacg gcctctaccg catcaagctg
     3121 aatgccaagg gctttgccgt cgtcaagctg aagtagcgtg ggctgtgccc acgatcggca
     3181 gaagtagcca gtggaattca gaagttccct gtcccgcggg cgacgacggc gatgttgacg
     3241 ctggcggcgc cggcagcaag cagcgtgcgg gccgcttcgt gggcggtagc gccggtggtc
     3301 atgacatcgt cgatgaccag gacgcgggcg cctgtgacat tcgagagcct ggagacgcgg
     3361 aacgccccgc ggacgttggc ccgccgctcc tcgggcaaga gccgcgattg ccggcggagc
     3421 atccggcggc agacgagcag gccgccggaa atgcgcgttc ccagggcgcg agcaagggac
     3481 cgggccagcg tgtccgcggc attcgtgccg cgccacatcc gctgcagcca gtgcatcggc
     3541 acgggtgcaa tcaggtcggg cggctggaag agcggctgct gctgcattcg ttcggccagg
     3601 agctggccga gcgccgccgc cagcggctcg tggtgatagt gcttgatccg taggaccacg
     3661 tcgcgcagcg gaccttgata gacgcccagc gcccggacct cttcgaagag cagccgccgg
     3721 ccgcggcagt cgtagcagtc cttcccgtca gcatctgccg cggggcaagg catcgcgcag
     3781 cgtctgcagg ccggcagggg cgacggctgg agctctgcca ggcaacttgc gcaaagcagc
     3841 ggcgtcccgt cccgggaccg acactcctgc ccacagctcg cgcagcgagg cggaaagagc
     3901 aggtctgtgg ttgccccgta gcctgcggcc agccagcggg gcgaacggcg gagaacactt
     3961 tcatgcgcag ccgacgggcc ggcagaatcg cccggattgc tagcatcggc agccattcgt
     4021 cttgaccctg ggaagctggt ggtcctatcg taacgcccgc gctagagcct tcccatgcgc
     4081 ccgcccgatt tcagaacggg ggaggcaaga ttgtcgtcgt tcgctccgcg aacgaacgcc
     4141 ctttcgcgga gcgaaaggcg acacaagcgt ccgccttcac gcgccatgcc caagatcatc
     4201 gaccgcgccc tggtcctgtt gttcctcaag tcgctgctgg tctgcttcgt cagcctggcg
     4261 ggactgtcgt tcatcgtcga cctggcgaac aacgccgagg agtttctggg ctatgtcaag
     4321 caacacggcc cgctggtgct gctcgattat tatgggccgc ggtcgctgct cttcttcgac
     4381 cggacggctg gcctcttggc gctcgtttcg acgatcttcg cgatcacgct catgcagcga
     4441 tcgaacgaat tcacagccct gatggccgcc ggcatcggcc cggcgcggat catcc
//
LOCUS       DASUGB010000403         4229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1156152, whole genome shotgun sequence.
ACCESSION   DASUGB010000403 DASUGB010000000
VERSION     DASUGB010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156152"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFV87_07745"
     CDS             complement(<1..509)
                     /locus_tag="VFV87_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103684.1"
                     /translation="MAVSNRSRWLLVLAALVGIVLTAYIARSRVEHWTRAQLAARHQR
                     QLARLAVEDAVLLVARLAEADDEYLDIVVLALADSRPQVTDAARQALAGRVQLWSHWP
                     VGDSSPRVAALADLLARSAEQIPAERRWFTQSLAAQLLVWPVDGRGVDTQQLIADCER
                     VLRLPTVQP"
     gene            858..1643
                     /locus_tag="VFV87_07750"
     CDS             858..1643
                     /locus_tag="VFV87_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103685.1"
                     /translation="MAAATLPLRSSIAAEEAADTSARARSEATRLLPLDELTAETRRK
                     LMAVVERPTIFRRLPHKSFDCDPELYLFLIRNPEVVVSIWDVMGISNMAAQRLGPYQW
                     KGSDGSGTECDIELVYGTDELHVLYGDGHYSGPLLKRKITGRSVLILSSGYGQAADRR
                     WQVGNRLDVFLQLDDAGADLVARTISPWVGKVADSNFAESCKFASRLSLTAEQNADGM
                     QRLSEKLTKVEPPVRQELARISAAIGQRAETRVLAAGVPERRG"
     gene            complement(1667..2266)
                     /locus_tag="VFV87_07755"
     CDS             complement(1667..2266)
                     /locus_tag="VFV87_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103686.1"
                     /translation="MRSAESGELIAKECDGAALHLEFVWHGDRFGHVISLIETSGRRV
                     PLLESVEGTADESSSSPPSPPLQSLSLDPLPEGRRVALLVGMAGRDHWSASIEPLPDA
                     AGFAFDIACRCSRGDIELASAYRLALDHRDLFAAADRSWIEARLPDGRSLVVTANGSA
                     APSDLELKNPTTFTIAPRAGQGNATIRWKYCLQLLPSDS"
     gene            complement(2288..3416)
                     /gene="prfB"
                     /locus_tag="VFV87_07760"
     CDS             complement(join(2288..3346,3348..3416))
                     /gene="prfB"
                     /locus_tag="VFV87_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_150074165.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5103687.1"
                     /translation="METDWYNRAEAIQKRITQLKDSLDYEGKLAHVAEIEDRMGAADF
                     WNNQERAREVVSQLKSLKALTRPLEDVVKSGADLPGMLELAEADESFAGEVRGEIERL
                     EVALDELELKSLLSGPHDAAGALVSIYARDGGTDANDWAEMLLRMYTLWAQKNDYDVS
                     ILERQDDAVAGIQSATIAVRGPMAYGYLKGETGIHRLVRISPFNSEGKRQTSFAAVDV
                     SPEVGEEAEIEIDWDKDVREETMRAGGAGGQHVNKTESAIRLLHYATGIEVKCQNERS
                     QHQNRAMARKMLLARLVRREEERREAEQAAKHKLQPKVGFGSQIRNYFLHPSQRVKDS
                     RTGYLEHHFDRVLDGNLQGFLDSYLKWRVKGEMAGTADDED"
     gene            complement(3777..>4229)
                     /locus_tag="VFV87_07765"
     CDS             complement(3777..>4229)
                     /locus_tag="VFV87_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103688.1"
                     /translation="YWPSVGEVTELGRTNYVGVAGYWGNVPTSAEARKYVGVFSNRTF
                     NGFASIEDGTSNTLLFGEATGGKDDEDDAVTEVFGLTWMGCGSFVTGGGLTTRNWFTF
                     NSNHPNIVQFCMADGAVRKVNRGIDNTMYTRILAGMRDRQAASLADVQ"
BASE COUNT          748 a         1395 c         1293 g          793 t
ORIGIN      
        1 tcaggctgca cggtcggcag gcggagcacc cgctcgcagt cggcaatcag ttgctgggtg
       61 tccacacctc gcccgtcgac gggccagacg agcagctgcg ctgcgagact ctgggtaaac
      121 cagcggcgct ccgctggaat ctgctcggcc gaacgagcca acaggtccgc caacgccgcg
      181 acgcgcggcg aggaatcgcc aaccggccag tgcgaccaga gttgcacgcg cccggcgagc
      241 gcctgccggg ccgcgtctgt gacctgcggc cgcgaatcgg ccaatgccag cacgacaatg
      301 tcgaggtact catcatcggc ttcggccagg cgcgcgacaa gcagcaccgc gtcttcaacg
      361 gccagcctcg ccaactgacg ctggtggcga gcggcgagct gcgcgcgggt ccagtgttcc
      421 acgcgcgatc gcgcgatata ggcggtcagc acgattccca ccagcgctgc cagcaccagc
      481 agccagcgcg agcgatttga aacggccatc ggcttcctcc atgactgcca aggcaggcgg
      541 ccattctcgc gatctgccga accagctcaa gatcagccgc gtagctctct cactcaatgc
      601 cgggatgcag cgatcccctt ggcggcttgc tagcacgctg ctttcagcgg ccgccattcg
      661 ttaaggtttt gctggtccgc tcagaggccg gcagccgaag aacctctagg accgtcacag
      721 cctgccctgc cgcttccacg gctagggtga agctgttgca ccaagggggg agcatgggcg
      781 tcttggcatc actgcgctgc gattctggtg gcaggctacc gtcgcgccgc acctttttga
      841 ccgggtgcgt ggcgggcatg gccgccgcca cgcttcctct gcgaagctca atcgccgccg
      901 aagaggcagc cgatacatcg gccagggccc gcagcgaggc gacgcgcctc ttgccgctcg
      961 atgagctcac ggccgaaacc cgccgcaagc tcatggccgt ggttgaacgc cccacgatct
     1021 tccgccgcct gccgcacaaa tcgttcgact gcgatccgga gttgtacctg ttcctgatcc
     1081 gcaacccgga ggtcgtcgtc agcatttggg acgtcatggg catctcgaac atggccgccc
     1141 agcgactcgg cccctatcaa tggaaaggga gcgacggctc cggcaccgag tgcgacatcg
     1201 agctggttta tggcaccgac gagttgcacg tgctgtatgg cgacggccat tattccggac
     1261 cgctgctgaa acggaaaatc accggccgca gcgtgctgat cctcagctcc ggctacgggc
     1321 aagccgccga tcggcgttgg caagtgggca accggctgga tgtatttttg cagctcgatg
     1381 acgccggcgc cgacctggta gcccgcacca tttcgccgtg ggtcggcaaa gtggccgact
     1441 cgaatttcgc cgagtcgtgc aagtttgcct cgcggctctc gctcaccgcc gaacagaacg
     1501 ccgatggcat gcagcggctg tcggagaagc tgacgaaagt cgagccgccg gtccgccagg
     1561 aactggcccg catctcagcc gccattggcc agcgggcgga aacgcgggtg ctggccgcgg
     1621 gagtgccgga gcggcgggga taggggttgg gttcagagtt cagggttcag gagtcggacg
     1681 ggagaagttg aagacagtac ttccagcgaa tggttgcgtt gccctgccca gcgcggggcg
     1741 caatcgtgaa cgtcgtcgga ttcttcaatt ctaagtccga cggcgcggcg gaaccattcg
     1801 ccgtaacgac gagtgacctg ccatcaggca aacgtgcctc gatccacgac cggtcggcgg
     1861 ctgcgaagag atctcggtga tccaaagcca atcggtaggc gctggccagc tctatgtcgc
     1921 cgcgagaaca gcggcaagcg atgtcgaagg caaagcctgc tgcgtctggc agcggttcga
     1981 tgctggccga ccagtgatcg cggccggcca tcccgaccaa tagggcaacc cggcggccct
     2041 ccggcagcgg atccaggctc aggctctgca gcggcggact cggcggtgaa gacgacgatt
     2101 catccgccgt gccttcgacc gactccagca gtggaacacg ccggccgctt gtttctatga
     2161 gcgagatgac gtgcccgaag cgatcgccgt gccaaacgaa ctccaggtgc agcgcagcgc
     2221 catcgcactc tttcgcgatc agctctccac tttccgcact tcgcattcca cactccgcac
     2281 tcgcccgcta gtcctcgtca tccgccgttc ctgccatctc ccccttcacc cgccatttca
     2341 gatacgaatc gaggaagccc tgcaggttgc cgtccagcac gcggtcgaag tggtgctcca
     2401 ggtaacccgt gcggctgtct ttgacgcgct gcgaagggtg caggaaatag ttgcgaatct
     2461 gtgacccaaa gccgaccttg ggttgcagct tgtgcttggc cgcctgctcg gcttcgcgcc
     2521 gctcttcctc gcggcggacg aggcgggcca ggagcatctt gcgggccatg gcccggttct
     2581 ggtgctggct ccgctcgttc tggcacttca cttcaatgcc ggtcgcatag tgcaagaggc
     2641 gaatcgcact ctccgtcttg ttgacgtgct gaccgccggc gccgccggcg cgcatcgttt
     2701 cttcccgcac gtccttgtcc cagtcgatct caatctccgc ttcctctccg acttccggcg
     2761 agacatcgac cgctgcaaag ctcgtctgcc gcttgccttc ggaattgaat ggactgatcc
     2821 gcacgaggcg atgaatgccg gtctcgccct tcaaatagcc gtaggccatc ggcccgcgga
     2881 cggcgattgt cgcgctttgg atgccggcca cggcatcgtc ctgccgctcc agaatcgaca
     2941 cgtcgtagtc gttcttctgg gcccacaggg tgtacatgcg aaggagcatc tcggcccagt
     3001 cgttggcatc ggtcccgccg tcgcgggcat agatactgac cagcgcgcca gccgcatcgt
     3061 gcggaccgct gaggagcgat ttcagctcca gttcgtcgag agccacttcg agccgctcga
     3121 tctcgccgcg cacttcgccc gcgaacgact catccgcttc ggccagctcc agcataccgg
     3181 gcagatcggc gcccgacttg acaacgtcct ccagtgggcg ggtcagcgcc ttgagcgatt
     3241 tgagctgcga gaccacttcg cgggcccgct cctggttgtt ccaaaagtcg gcagctccca
     3301 tgcggtcttc gatctcggca acgtgcgcca gcttgccttc gtagtcaaag actatccttg
     3361 agctgcgtga ttcgcttctg gatcgcttcg gcccggttgt accaatccgt ttccatgtgc
     3421 tgttccaatc tgcaaatctg gttgcgaaaa gctgggcgct ccgagggcaa tggcgatgca
     3481 ggtttccttt ccaagtgtag ggcgaatgca caggcgggag aatggcgcct ccggtgtggt
     3541 ggttatcacg cctgcgcggt catggattgg ccggcgcttg aaatccactg gatttagact
     3601 gcggcggagg aagcaccggg cctttagccg cctcctcggc tgtgggtatg cgctgccccg
     3661 atccgcaccc cgggaacccc ggaccagcga cgagcaacgc cacggcaagc gatcgaatcc
     3721 aattcatagc aaatcctcgt gctgaaaggg aatggcgaag gcgtccgcgg cgatcactat
     3781 tgaacgtctg ccaggctggc agcctgccga tcgcgcatgc cggccagaat gcgcgtgtac
     3841 attgtgttgt caatgccgcg attgaccttg cggacggccc cgtcggccat gcagaactgc
     3901 acgatgttgg gatggttgct attgaacgtg aaccagttgc gggtcgtcaa accgccgccg
     3961 gtcacgaaag atccgcagcc catccacgtg agcccgaaga cctccgtcac agcgtcgtcc
     4021 tcgtcgtcct tgcctcccgt ggcctctcca aacagcagcg tgttgctcgt tccatcttca
     4081 atcgacgcga atccgttgaa cgtccgattg gaaaatacgc ccacatattt tcgcgcttcg
     4141 gcactggtcg ggacgttccc ccaatagccc gcgaccccga cgtaattggt gcgccccagt
     4201 tcggttacct cgccgacgct gggccaata
//
LOCUS       DASUGB010000404         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_879252, whole genome shotgun sequence.
ACCESSION   DASUGB010000404 DASUGB010000000
VERSION     DASUGB010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879252"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFV87_07770"
     CDS             complement(<1..731)
                     /locus_tag="VFV87_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103689.1"
                     /translation="MPSVLIAATLLFNAPPAGVSGAGTSALPPIVALVVSPDGQHVLA
                     GSQVGIQILSLPSLRPAEGVAKIDTGLAQVHDLAFSPGGKLLAMAGGAPAEEGSVELW
                     DWPAVDKRTTIAAGGDVAYAVAWSPDGSRLAIAGGDHQLRIQPVDGSPAKSYAIHSAP
                     ILATGWLAGDDDLVLSAGVDQAIRVLDPAGGQVRRSLDNHTAAVRGLAVRPGKHDGPP
                     MVASAGADRTVRFWQPAIGRLVRFA"
     gene            complement(761..>2018)
                     /locus_tag="VFV87_07775"
     CDS             complement(761..>2018)
                     /locus_tag="VFV87_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103690.1"
                     /translation="IAVGFGGDVLRASGVKHVTISDVVLRGATGSPLMHVYGCENIQL
                     DHVTAFGGFPGLLVNASKDVRVTHCAFRGLAASWTSRAHMKYRGTASYQVILQDNQPT
                     NENIEFSHCEFTDDHDFAFLRPAKNLQFHHNFVDNFNDDGLECGPKLRDHTIYIWQNR
                     LGHCLIPLSQHEIRQDESPADQNPGSGVFVFRNVIDLRGGTYKGPPPQPDSSGAYLRQ
                     EGHLASDHGGPIWPVIRFYHNTVLRQTPVFRDYFLFGLGAQGLRGTERDVFNNIFVQA
                     QGVPGVGFAGIKEPGNVREGGNLLWGLADGPARKTNPLAKFRESPLFIASRKHYQPGL
                     TTQDLVADPQFVAFAEDEAAAVDLRLKAGSPAIGAGQPIPGVWPDPLRAADKGQPDIG
                     AIPGDAAAWGVGVDGRIALFSGAVSE"
BASE COUNT          375 a          658 c          641 g          344 t
ORIGIN      
        1 tttgcaaacc ggaccagtcg gccaatagcc ggctgccaga accggacggt gcgatcggct
       61 ccggcggaag cgaccatggg cggcccgtcg tgctttccgg ggcgcaccgc caggccgcga
      121 accgctgccg tgtgattgtc gagcgagcgg cggacctggc cgcccgccgg atcgagcacc
      181 cggatcgcct gatcgacgcc tgctgaaagc accaggtcgt cgtcgccggc cagccatccg
      241 gtcgcgagga tgggagctga gtgaatcgca tagctcttgg cgggcgagcc atccaccggc
      301 tggatgcgca gctgatggtc gcctccggcg atggccaggc gcgacccatc ggggctccaa
      361 gcgactgcgt aggcaacatc gccgccggcg gcgatcgtcg tccgcttgtc taccgccggc
      421 caatcccata gttcgacgct gccttcctcg gccggcgcgc cacccgccat cgccagcagc
      481 ttgccgccag gtgaaaacgc cagatcatga acttgggcca gtccagtatc gatcttggcg
      541 acaccctccg cggggcgcag cgacggcaac gacaaaatct gaattcccac ttgcgagccc
      601 gccagcacat gctggccatc gggcgaaacg accaaggcca caattggcgg cagggctgat
      661 gttcctgcgc ccgagacgcc cgcaggcgga gcattaaaca gcaacgtcgc ggcaatgaga
      721 acggacggca tagacaaatg gaaccagttg atcgcggcgc ctattctgaa acagcgccgc
      781 tgaacagtgc aatccggcca tcgactccaa cgccccaggc ggcagcgtcc cccggtatgg
      841 cgccaatatc cggttgtccc ttatccgctg cgcgcagggg atcgggccag actcccggaa
      901 tcggctgccc cgcgccgatc gccgggctgc ccgctttcaa tcgtaggtcc accgccgcag
      961 cttcatcttc cgcgaaggca acgaattgcg gatcggccac caggtcctgc gtcgtcagcc
     1021 ccggctggta gtgtttgcgg ctggcgataa acagcggcga ctcccggaac ttcgccagcg
     1081 gattcgtctt tcgtgccgga ccatcggcca atccccacag cagattgccc ccctcgcgga
     1141 cgttgcccgg ctctttaatg ccggcaaagc cgacgccggg cacgccctgg gcctggacga
     1201 agatgttgtt gaacacgtcc cgctcggttc cgcgcagtcc ctgcgcgccc aagccgaaca
     1261 ggaagtaatc gcgaaacacg ggcgtctggc ggagcaccgt gttgtgatag aagcgaatca
     1321 cgggccagat cggcccaccg tgatcgctgg ccaggtgccc ctcttgtcgc aggtacgctc
     1381 ccgaggagtc cggctgcggc ggcgggccct tgtacgttcc cccgcgcagg tcgatgacgt
     1441 tgcgaaaaac gaacacgccg ctgcctggat tctggtcggc cggggattca tcctggcgga
     1501 tttcgtgctg cgagagcgga ataaggcagt ggccaagtcg attctgccag atgtaaatcg
     1561 tgtgatcgcg aagcttgggt ccacattcca ggccgtcgtc gttgaagtta tcgacgaaat
     1621 tgtggtggaa ttgcaggttc ttcgcgggcc gcaagaacgc gaaatcgtga tcgtcggtga
     1681 attcgcagtg agaaaactcg atgttctcgt tggtgggctg gttgtcctgc aggatgactt
     1741 gatatgaagc cgtgccccgg tatttcatgt gggcccgcga ggtccacgac gccgccaggc
     1801 cgcgaaaagc acagtgggtg acgcgaacgt ccttcgatgc gttgacgagc aggccaggaa
     1861 agccgccgaa ggcggtcacg tgatccagct ggatgttctc gcagccatag acgtgcatga
     1921 gcgggctgcc ggtcgccccg cggaggacaa cgtcgctgat ggtgacatgc ttcaccccgc
     1981 tcgcccgcag cacgtcgccg ccgaatccga ccgcgatc
//
LOCUS       DASUGB010000405         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1018215, whole genome shotgun sequence.
ACCESSION   DASUGB010000405 DASUGB010000000
VERSION     DASUGB010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018215"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFV87_07780"
     CDS             complement(<1..672)
                     /locus_tag="VFV87_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103691.1"
                     /translation="MDFRRGQLDERRRRVLMDEIASPPPAADSPEAQPAPAGSAKGPV
                     RAYSSAVLADRQPKVTDFVPVRPFAAIILVLLGLTGVAAIETIHIYLQTLASGPPPEL
                     LAALDVSRTGSLAAWFSSLLLALGAVGALVIFGVRAHRVDDYRGRYRIWLWTAAALGW
                     ASLDATTQMHGALGFGVSVFALQQLGGPSAAAAVTMGWLAVYALLLGTLAIRVAIEIC
                     PSLPSL"
     gene            complement(822..1340)
                     /gene="tpx"
                     /locus_tag="VFV87_07785"
     CDS             complement(822..1340)
                     /gene="tpx"
                     /locus_tag="VFV87_07785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX5103692.1"
                     /translation="MSRPNAVTFKGTPLTLAGEALKVGQPAPNFTIHYFEGGLKTITP
                     ADLKGKPTILSVVPSLDTPVCATQTKKFNDQLAALGDKINAVTISLDLPFAMNRFCGA
                     ESIKNMRCGSDYQDRNFGKSYGFLIEELKLLARGVVILDKDGKVVYSETVPEVTSEPN
                     YDKALEALKGAL"
     gene            1556..1936
                     /locus_tag="VFV87_07790"
     CDS             1556..1936
                     /locus_tag="VFV87_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5103693.1"
                     /translation="MLLELSPEWTMDLDRGPDWMLLRLRPPAKGDGAPFPLADMISKK
                     LEQSFCYRLVLELDDVRVLRSWMLGELVRLNKRIDEKGGTLRLCGLSDANEAALAACG
                     LFEHFPSFENRHDAVMGCRPKQPR"
     gene            complement(1941..2054)
                     /locus_tag="VFV87_07795"
     CDS             complement(1941..2054)
                     /locus_tag="VFV87_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5103694.1"
                     /translation="MLQGVVKTYFQKQMAQETVRRVAGVEEVENCLEVTWT"
BASE COUNT          397 a          635 c          660 g          381 t
ORIGIN      
        1 caaactcggc agcgacgggc agatctcgat cgcgacacga atcgccagcg ttcccagcag
       61 cagcgcataa acggccagcc aacccatcgt gacggctgcg gccgcgcttg gcccccccag
      121 ttgctgcaga gcgaacaccg aaacgccaaa tcccaaagcc ccgtgcatct gcgttgtcgc
      181 atcgaggctc gcccagccga gcgccgcggc cgtccagagc caaatgcggt atcggccgcg
      241 atagtcatcg acgcgatgag cgcggacgcc gaagatcacc agcgcgccca ccgcgcctag
      301 ggccagaagc agcgatgaaa accaggccgc caggctgccc gtccgactga cgtcgagcgc
      361 cgccagcagt tcgggaggtg gcccgctggc cagcgtctga agataaatgt gaatcgtctc
      421 gatcgccgca acacccgtca gcccgagcag caccaggatg atcgcggcaa aagggcgaac
      481 gggaacgaaa tcggtcacct tcggctggcg atcggcgagg acggcggagc tataggcgcg
      541 gaccggaccc ttcgccgagc cggcgggcgc gggctgcgcc tcggggctgt cggctgcggg
      601 tgggggcgaa gcgatctcat ccatgagaac gcgccgccgc cgctcgtcga gctggccgcg
      661 tcgaaaatcc attttcggaa aggcgctgcg tgggagggtc ggtcaggggc agggcatgcg
      721 cctcagaggg acaattcggc agcccgcacg tgcaatctgc actttggtca ttccggcaga
      781 atcagcggga aaaccacacc cgagtttgcc ggttagctcg actacagggc gcccttcagg
      841 gcttcgagcg ccttgtcgta gttcggctcg ctcgtaactt ccggcacggt ctccgaatag
      901 acgactttgc cgtccttgtc gaggatcacg acaccgcgtg ctagcagctt cagttcctcg
      961 attaaaaagc cgtaactctt accgaagttt cggtcctggt agtcgctgcc gcagcgcata
     1021 ttcttgatcg actcggcccc gcaaaagcgg ttcatggcga acggcaggtc gagactgatc
     1081 gtaaccgcat tgatcttgtc gcccagggcg gcaagctggt cattgaactt cttggtttgc
     1141 gttgcacaga cgggcgtatc gagcgacggc acgacgctga ggatcgtcgg cttgcccttg
     1201 aggtctgccg gcgtaatggt cttcaatccg ccctcgaaat agtgaatcgt gaaattaggg
     1261 gcaggctgtc cgactttgag ggcttcgccg gctagcgtga ggggagtgcc tttgaacgtg
     1321 acagcattag gtcgcgacat ggggatcgac tcctacggga acgaagtcag gtgtgcgaat
     1381 tcgggcaaaa tgttgcccca gtattgcgcc ctcccgcagc gctcacaagg ggccaaagca
     1441 gattgctcgt cctgccattg attccgacgc aagcgggcgt aagatcaaca tctggttggt
     1501 agggttatct cgtgtcggcc ctcttctttg gccgaccagc aatggaggcg tagccatgct
     1561 tctggaattg agtccagaat ggacgatgga tttggatcgc ggaccggatt ggatgctgct
     1621 gcggctgcgt ccgccagcga aaggcgacgg cgcgccgttt cctctcgccg acatgatctc
     1681 gaaaaagctc gagcagtcgt tctgctatcg gctggtgctg gagttggacg acgtgcgcgt
     1741 gctgcggagc tggatgctgg gcgaactcgt ccgcttgaac aagcggattg atgagaaggg
     1801 cggaacgctg cggctgtgcg gcctttcgga cgcgaacgaa gcggccctgg cggcctgcgg
     1861 gctgttcgaa cacttcccgt cgttcgagaa ccgccacgac gccgtcatgg gctgccgccc
     1921 caagcagccg cgataaccgc tcaggtccag gtgacttcca ggcagttctc aacctcttcc
     1981 acgccggcca cccggcgcac ggtttcctgc gccatctgct tttggaagta ggtcttcacg
     2041 accccctgca gcacgacgcg gctgccgtcg gtt
//
LOCUS       DASUGB010000406         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1433065, whole genome shotgun sequence.
ACCESSION   DASUGB010000406 DASUGB010000000
VERSION     DASUGB010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433065"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..736
                     /locus_tag="VFV87_07800"
     CDS             236..736
                     /locus_tag="VFV87_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX5103695.1"
                     /translation="MKFKARVWNVGDNINTDLILPITVFYETPEKQARMVFSANRPGW
                     VDQVQKGDILIGGRNFGMGSSRPAARSLKNVGLGCVVAHSLNGLFYRNCINFAFPGLE
                     CPGVDQAFEEGDVAEVDFEASVVRNVTRNVTLQARPTPPRLLDLLKAGGIYPLLEKEG
                     LIAPKA"
     gene            complement(798..1745)
                     /gene="cynR"
                     /locus_tag="VFV87_07805"
     CDS             complement(798..1745)
                     /gene="cynR"
                     /locus_tag="VFV87_07805"
                     /inference="COORDINATES: protein motif:HMM:NF008416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HEX5103696.1"
                     /translation="MELRHLHYFLSIAESSSFTRAAAMLHVTQPTLSHQIRQLEQEIG
                     KPLFDRVGRGVRLTEPGKVLREHARRAVKEIQSGLRAVAELDGLVRGTLALGVFRTYS
                     SSPLPDVLAQFSSRHPGVEVTVKQLSLRDIERELVEGGIHMGIGYLPITSDRLVVEPL
                     FAVPLALVVSRTHPLFGRLKVSISDLQAQPLVLLSRDFPLRQLIDRRLAHHGVTPRIV
                     MEMNANDAVLSTVRAGALATILTARELRGAANLRSVPIDDPQLTRTAAILWDRDAYRP
                     AAAQALARLVKRAYADADRSTRQPRGRLIRRKNRPEEEE"
     gene            1904..>2357
                     /locus_tag="VFV87_07810"
     CDS             1904..>2357
                     /locus_tag="VFV87_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5103697.1"
                     /translation="MDVSLNEEQRAWQAKARRFAQDVIAPVSLERDRIAGAPETFDWD
                     IIRQGSKLGFRTAVVPKEYGGHGIDLVTQALVITELAKADSAISKTFSQCWKWSHLIA
                     DFCSKDQKTRFLAPFMADDTYLLGHAGTEPNAGSDHRLPPEDDPKSGWR"
BASE COUNT          423 a          762 c          781 g          391 t
ORIGIN      
        1 acgaggtgtg catcacctcc agcacgcgca acttcaaggg ccgcatgggc gagccgagct
       61 cgaaggtcta catgggctct ccggcgacgg tggccgcatc ggcgatccgc ggcgtcatca
      121 ctgatccccg ggaattttta aactgatgct ctcagccgtc agcattcagc tgtcagccaa
      181 aaccaaaggc ctgtagctga aagctgatag ctcatcgctc aaagctgagg attgtatgaa
      241 attcaaagca agagtctgga acgtcggcga caacatcaac accgacctca tcctcccgat
      301 cacggtgttc tacgagacgc cggagaagca ggcgcgcatg gtgttcagcg ccaaccgccc
      361 gggctgggtg gaccaggtgc agaagggcga catcctgatc ggcggcagga acttcggcat
      421 gggctcgagc cggccggcgg cgcgctcgct caagaacgtc ggcctcggct gcgtcgtcgc
      481 gcattcgctg aacgggctgt tctaccgcaa ttgcatcaac ttcgccttcc cggggctgga
      541 gtgcccgggc gtggaccagg cattcgagga gggtgacgtc gccgaggtgg atttcgaagc
      601 atcggtcgtg cgcaacgtca cccgcaacgt cacgctccag gcccgtccca cgccgccgcg
      661 gctactcgac ctgctcaagg cgggcggaat ttatccgctg ctggagaaag aaggactcat
      721 cgctcccaaa gcgtgacggg atacaggggg cagggccgcc ccgtttttcc agtcaccgcg
      781 aggggtgacc gtacgcttca ttcctcttct tccggcctgt tcttcctgcg gatcagcctt
      841 ccgcgcggct gccgcgtcga gcggtccgcg tccgcatacg cgcgcttcac cagccgggcc
      901 agcgcctgcg cggcggcggg ccggtaggca tcgcggtccc acaggatcgc tgccgtccgg
      961 gtgagctgag gatcgtcgat cggtactgag cgaaggttgg ccgcgccgcg cagctcgcgt
     1021 gccgtcagga tcgtggcgag cgccccggcg cgcacggtgg agagcacggc gtcgttggcg
     1081 ttcatctcca tcacgatgcg cggcgtgacg ccgtgatgag ccaggcgacg gtctatcagc
     1141 tggcgcaacg gaaaatcccg gctgagcagg accagcggct gggcctgcag atcgctgatc
     1201 gagaccttga ggcgcccgaa gagcgggtgc gtcctgctga ccacgagcgc gagcggcacg
     1261 gcgaagagcg gctcgaccac cagccggtcg gacgtgatcg gcaggtagcc gatgcccata
     1321 tggatgccgc cctcgacgag ctcccgctcg atgtcgcgca gtgagagctg cttgacggtg
     1381 acctccacgc cggggtgccg gctgctgaac tgcgccagga catccggcag cggcgagctg
     1441 ctgtaggtgc ggaacacgcc gagcgcgagc gtgccgcgca cgagcccgtc gagttcggcc
     1501 acggcgcgca gccccgactg gatttccttg accgcgcgcc gcgcatgctc tcgcaggacc
     1561 tttcccggct ccgtcagccg cacgccgcgg ccgacgcggt cgaagagggg cttgccgatt
     1621 tcctgctcga gctgccggat ctggtgcgac aaggtaggct gggtgacgtg cagcatcgcg
     1681 gccgcgcgcg tgaagctcga gctttcggcg atgctcaaga agtagtgaag atgacgcagt
     1741 tccacggccg gcctcccgat tccagatatc gatatcatca atgagtttaa taaagaaaaa
     1801 gcatttgctc tatagcacgc gccgctataa ctttgaatcg cgccgcgcga gcgggggatg
     1861 tggggaaccc tgcccgctgc ggcgtgtttc cgaaggagga gccgtggacg tatccctcaa
     1921 cgaggagcag cgcgcgtggc aggcgaaggc ccgccgcttt gcccaggacg tgattgcgcc
     1981 ggtttctctg gagcgcgacc gcatcgccgg cgccccggag actttcgact gggacatcat
     2041 ccggcagggc tcgaaactcg gcttccgcac ggccgtcgtg ccgaaggaat acggcgggca
     2101 cggcatcgac ctcgtcacgc aggcgctggt catcaccgag ctggccaagg ccgacagtgc
     2161 catctccaag accttcagcc agtgctggaa gtggagccat ctaatcgcgg atttctgcag
     2221 caaggaccag aagacgcgct tcctcgcgcc gttcatggcc gacgacacgt atctgctggg
     2281 gcatgccggg acggagccga acgccggttc cgaccaccgc ctgccgcctg aggacgaccc
     2341 gaagtccggc tggcgcc
//
LOCUS       DASUGB010000407         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1156537, whole genome shotgun sequence.
ACCESSION   DASUGB010000407 DASUGB010000000
VERSION     DASUGB010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156537"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFV87_07815"
     CDS             <1..817
                     /locus_tag="VFV87_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103698.1"
                     /translation="WTVGKSADQLTDYLSYALRILKNAGLHCEGITTPGGFGGKVLPE
                     LAQAVLQSCREVHGTEIPHYFRHAFLDDRSVAPRVEYATGLDGPDPRCVVSIIGCTND
                     WFGGWDAVVEGDVNQMVTPDLKSGRMLDVIRRGEPAVMYCHWPGIYFNGTESGFNIIK
                     EIHRRLHASHDNLIWMKLSELARYWAAKELTTISAAGAGADFKAPYACPEFTVKLSSW
                     TTTAAPKLRAGQQQTPLREVSSPLQLKGPSWARQGAGIIACFDLPKGASQLSA"
     gene            complement(814..1755)
                     /locus_tag="VFV87_07820"
     CDS             complement(814..1755)
                     /locus_tag="VFV87_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5103699.1"
                     /translation="MPDRRQFLLSLPALVSCGGRHGKLIAVVPKAVSHLFFVAVHAGV
                     KRAARETGVEVLWNGPNDETDHARQIQIVESMITQRVDALAISATDERALSATVQRAI
                     QAGIPVSIFDSGVNVEGYVTFVATDNYQAGVTAAREMARLCSGNGDIGMVMHKPGGTS
                     TMLREQGFEETVAKELPGIKIVARQYGMADRAKARGAAENILTAHPDVAGLFASSEAS
                     SLGAIQAISTRGLSGEVRLITFDTSESHVEALKNGTIDTMLVQDSFRIGYEAVKSLSD
                     KLHGRTPQRRMDLPARVITKADLDKPEVQSLVKPEVG"
     gene            complement(1791..2351)
                     /locus_tag="VFV87_07825"
     CDS             complement(1791..2351)
                     /locus_tag="VFV87_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103700.1"
                     /translation="MSRAERPYASVHGKAVAEESLANGYKLEHPTLLFEDRMAFDDGE
                     RRVELIRVGPGHTIGDAVAYLPKEKILAVGDLCVNWTSGNNTGDRDADLDNWVTVLGK
                     LAEWDAKTIVPGQGKLGTADTLRGQQAYLTELVSKVRAGVRNGNTADQLAQGIDLSRH
                     QPWGANAASNANAVRSVYRRLAGQGK"
     gene            complement(2348..2740)
                     /locus_tag="VFV87_07830"
     CDS             complement(2348..2740)
                     /locus_tag="VFV87_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5103701.1"
                     /translation="MRRVAAAAAFVLLALIAARSQDQTVRELAPGVFFWRGDTNIRRP
                     ANCTWIIFKDYVLVIDTNFPWGAREILPEIRKTTSNPIRYVFDTQYHADHSFGNSIFK
                     EAGATIVCSRECGTESRRKGEADWRNQC"
     gene            complement(2737..>2936)
                     /locus_tag="VFV87_07835"
     CDS             complement(2737..>2936)
                     /locus_tag="VFV87_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103702.1"
                     /translation="LANITGHMTPAMVAAEVSKLPAGTRIVVTHLKAPFREEVAQELM
                     ALNIPCLELCESGREYDFGEP"
BASE COUNT          541 a          936 c          838 g          621 t
ORIGIN      
        1 atggactgtt gggaagtccg ccgaccaact caccgactac ctctcttatg ccctccgcat
       61 tctcaagaac gcgggactgc actgcgaagg catcaccacg ccgggcggtt tcggcggcaa
      121 agtgctaccg gaattggcac aggccgtcct gcaatcgtgc cgggaggtgc acggcacgga
      181 aatccctcac tacttccgcc acgcgtttct tgacgaccgc agcgtggccc cgcgggttga
      241 gtacgcaaca gggctcgatg gccccgaccc acgctgcgtg gtctcgatca taggctgcac
      301 caatgattgg ttcggcggct gggatgccgt tgtcgaaggc gacgtgaatc agatggtcac
      361 gccggatttg aaaagcggac gcatgctgga tgtcattcgg cgtggcgagc ctgccgtcat
      421 gtattgccac tggccgggta tttacttcaa tggcacggag tccggattca acatcatcaa
      481 agagattcac cggcgtctgc acgcatcgca cgacaacctc atttggatga agctaagcga
      541 acttgcgcgc tactgggccg ccaaagagct gacaacgata tctgcagcgg gggcaggcgc
      601 cgatttcaag gctccctacg cctgtccgga atttacggtg aagctctcca gttggaccac
      661 aaccgctgcg ccaaagttgc gtgcgggaca gcagcaaacg ccgctgcgtg aggtaagcag
      721 ccctctgcag ctgaagggac catcgtgggc gcggcagggc gccggcatca tcgcctgttt
      781 cgaccttcca aagggcgctt cacagttgag cgcctagccg acctccggct tcaccagcga
      841 ttgcacctcc ggtttgtcca ggtccgcctt cgtgatgacg cgcgcgggca gatccatccg
      901 gcgttggggt gtcctgccgt gaagcttatc tgacagcgat ttcacagcct cgtatccgat
      961 gcggaaggaa tcctgtacca gcatggtgtc gatcgtgccg ttcttcagcg cttcgacatg
     1021 cgattcgctg gtgtcgaagg tgattagccg cacttcgccc gagagtccgc gcgtcgagat
     1081 cgcctgaatc gctcccagcg aacttgcttc gctcgatgcg aacaggcctg cgacgtcagg
     1141 gtgtgccgtc aggatattct ccgccgcgcc gcgcgctttg gcgcggtcgg ccatcccgta
     1201 ttggcgcgca acgatcttaa tacctggtaa ttctttcgcg acagtctcct cgaatccctg
     1261 ctcccgtagc atcgtcgacg ttccgcctgg tttgtgcatc accattccga tgtctccatt
     1321 gccgctgcac agccgcgcca tttcgcgcgc cgcggtgacg ccagcctgat agttatccgt
     1381 cgcgacaaag gtgacgtagc cttcgacgtt cacgccggaa tcgaagatgc tcaccggaat
     1441 gccagcctga atcgccctct gcacagtcgc actcagcgca cgttcgtcgg tggccgagat
     1501 cgccagtgcg tcgacccgct gcgtgatcat cgattcgacg atttggatct ggcgggcatg
     1561 gtcggtctcg tcgttcggcc cgttccacaa cacctcgacg ccggtttcgc gcgccgcccg
     1621 cttcacgcca gcgtgaacgg cgacaaagaa caggtgcgaa accgccttcg gtaccacggc
     1681 gatcaatttg ccgtgtcttc ctccacagga cacaagcgcg ggcaacgaca gtaggaactg
     1741 gcggcggtcg ggcacgcgac tatcctacac gcgtccgtac gtcgaagcgg ctatttacct
     1801 tgcccagcaa gacggcgata tacagagcgc accgcatttg cgttcgatgc tgcgttcgcg
     1861 ccccaaggct ggtggcggga caggtcaatt ccctgagcca gttgatcagc tgtgtttcca
     1921 tttcgaacgc cggcgcgtac cttgctgacc aattccgtca ggtacgcctg ctggccccga
     1981 agtgtgtcgg ccgtgcccag tttgccctgc cccggaacga tggttttggc atcccactcg
     2041 gcgagtttgc cgagaacggt cacccaattg tccagatcgg cgtcgcggtc gcctgtgttg
     2101 tttccgctgg tccagttcac gcaaagatcg ccgactgcca ggatcttctc ttttggcagg
     2161 tatgcgacgg catcaccgat cgtgtgtccc ggacccaccc gaatcaactc cacccgccgt
     2221 tcaccgtcgt cgaatgccat acggtcttcg aagagcagcg tcggatgctc cagcttgtat
     2281 ccgtttgcga gcgactcttc ggctacggcc ttgccgtgca cggacgcata ggggcgctca
     2341 gcgcggctca acactgattc cgccagtccg cctccccctt gcggcgcgac tcggtgccgc
     2401 actcccgtga acagacgatg gttgcgcctg cttctttgaa gatactgttc ccgaagctgt
     2461 ggtcggcgtg atactgggtg tcgaatacgt accggatggg attgctcgtt gtctttctga
     2521 tctccggcag tatctcccgt gctccccaag ggaagttcgt gtcgatcacc agaacatagt
     2581 ctttgaagat gatccaggta cagttggctg gccggcgaat atttgtgtct ccccgccaga
     2641 agaacactcc cggcgccaac tctcgaaccg tctggtcttg actacgtgcc gcgatcagcg
     2701 cgagaagcac aaatgcggcc gcggccgcaa ctcgtctcat ggctccccga aatcgtactc
     2761 gcgcccagat tcacacaact caaggcatgg aatgttaagt gccatcagct cttgcgccac
     2821 ctcctcacga aacggcgctt tgagatgggt cacgacgatc cgcgtccccg ccggcagctt
     2881 gctgacctcg gcagcaacca ttgccggcgt catatgccca gtgatgttgg ccaatt
//
LOCUS       DASUGB010000408         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1990647, whole genome shotgun sequence.
ACCESSION   DASUGB010000408 DASUGB010000000
VERSION     DASUGB010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990647"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFV87_07840"
     CDS             complement(<1..902)
                     /locus_tag="VFV87_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5103703.1"
                     /translation="MSRLSRRRFVQAAAAASTAFPLFTIGGTKASGRVIGANDTIRVG
                     VAGIHGRGGSHLSAFTDMDKVAVTYLIDCDSSLFDSRSKNVEQRTGIRPNCVQDVRQA
                     LDDKTLDAVTVATTNHSHSLITVWACQAGKDVYVEKPISHNVFEGRKCVEAAKKYGCV
                     VQHGTQSRSETGWAKTVAAVHSGKYGKLLVSKGEASKNRWGIGFKANETPPATLDFNI
                     WLGPAQEQPFHRNLVHYNWHWFWDFGNGDIGNQGVHQMDIARWGIKGGTLPTKVYSLG
                     GRFLPDDKENSPEGKDQGQVPNQQL"
     gene            complement(1086..2294)
                     /gene="ribA"
                     /locus_tag="VFV87_07845"
     CDS             complement(1086..2294)
                     /gene="ribA"
                     /locus_tag="VFV87_07845"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5103704.1"
                     /translation="MPAFSTIQSAVDAIAAGAVIIVVDAEDRENEGDFICAAEKATPE
                     AINFLIGGRGQFCAPMLPEVAQRLELTPVVELNTAPLGTSFLTPIDHCSAKTGITAEE
                     RCRTVRAIADGATTADDFVRPGHVHPLLAKEGGVLRRAGHTEATVDLCRMAGLAPVGV
                     LCEILNDRGERATRDELHAIADKHKLKIISIEQLIAHRRVSEKLVSRITQAQIPTADY
                     GEFTIIAYDVKYESQQPLVLVKGEPDKAEAPLVRLHSSCFTGDLLDSLRCDCGSQLQM
                     ALRMIDEAGVGVLVYLPQEGRGIGLINKIRAYELQEKGADTVEANLALGYKADMREYG
                     VGIQILKDLGLRKVKLLTNNPKKVDAFIYDGFDLQVVDQVPIVAPTNPHNARYLSTKR
                     DKLGHKLPAS"
     gene            2422..2658
                     /locus_tag="VFV87_07850"
     CDS             2422..2658
                     /locus_tag="VFV87_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103705.1"
                     /translation="MLEREEYIEQAHLFRVLGERLADNVPMQELLDHVKQEVLSTTRL
                     PMAIDFLLTELRHIGMMSTAMERLPHYFTPFRRT"
     gene            2658..>3081
                     /locus_tag="VFV87_07855"
     CDS             2658..>3081
                     /locus_tag="VFV87_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103706.1"
                     /translation="MQEAEAEQGRFDLRIAVAILRHEAEYRAKSPIPQAMFMYQFECL
                     CRNRLKYDAGLAAVAGDPIYDENWQQWIRELRLRIGMVDFADMIYVRSQYNLIHRTRP
                     GEEPPPPEKPVLFGEKEGKIAWANRRKDPLYLFAALQRH"
BASE COUNT          522 a          917 c         1048 g          594 t
ORIGIN      
        1 agctgctggt tgggcacctg gccctggtcc ttgccctccg ggctgttttc cttgtcgtcg
       61 ggcaggaacc gtccgccgag gctataaacc ttcgtcggca gcgtaccccc tttgatgccc
      121 caacgggcga tgtccatctg gtggacgccc tggttgccaa tgtcgccgtt gccaaagtcc
      181 cagaaccagt gccagttgta gtgcaccagg ttgcggtgga acggctgctc ctgggcgggg
      241 ccgagccaga tattgaaatc gagcgtggcc ggaggcgttt cgttggcctt gaagccgatc
      301 ccccagcggt tcttggaagc ctcgcccttg cttaccagca gcttgccgta tttgccggaa
      361 tggaccgccg cgaccgtctt tgcccaaccg gtctcgctgc ggctctgggt gccgtgctgg
      421 acgacgcagc cgtacttctt cgcggcctcg acgcacttgc ggccttcaaa gacgttgtgg
      481 ctgattggct tttcgacata gacgtccttg cctgcctggc aggcccagac tgttatgagc
      541 gagtgcgagt ggttggtggt ggcgaccgtg acggcgtcga gcgttttgtc gtccagcgcc
      601 tggcggacgt cttgcacgca attggggcgg atgccggtcc gctgctcgac attcttgctg
      661 cgcgaatcga acagcgaact gtcgcagtcg atcaggtagg tgacggcgac cttgtccata
      721 tcggtgaagg ccgaaaggtg gctgccgccg cggccgtgaa ttcccgccac gccaacgcgg
      781 atcgtgtcgt tggccccgat cacccggcct gaggccttcg tcccgccgat cgtaaacagc
      841 ggaaaggccg tgctggcggc tgcggcggct tggacgaaac ggcggcggga gaggcgggac
      901 atggcaggct ccttgagagg gacgtaggag gacgtaggac ggaacaatgg tccgtcccca
      961 ggagaggtga ggcgggttgc aataactcgc tagaaattac agcacaccga ccagcgaata
     1021 gcaagcgcgc agccaagagc gcataagcca cgctgtcgat gtgggttgcg tggcacatcc
     1081 agccgttagc tcgccgggag cttgtggccg agtttgtcgc gcttggtaga gagataccgt
     1141 gcgttgtgcg ggttggtggg cgcgacgatg ggaacctgat cgaccacttg caggtcgaag
     1201 ccgtcgtaaa tgaaggcatc gaccttcttg gggttgttgg tgaggagctt cacctttcgc
     1261 aggccgaggt ccttgagaat ctggatgccg acgccgtact cgcgcatgtc ggctttgtag
     1321 ccgagggcga ggttggcttc gaccgtgtcg gctcctttct cttgcagctc gtaggcccgg
     1381 atcttgttga tcaggccgat gccgcggcct tcctgcggca gatagacgag gacgccgacg
     1441 ccggcctcgt cgatcatccg cagggccatc tggagctggc tgccgcagtc gcagcggagc
     1501 gaatcgagga ggtcgcccgt aaagcacgac gagtgcaggc ggacgagcgg ggcttcggcc
     1561 ttgtcgggct cgcccttgac cagcaccagc ggctgctgcg actcgtattt cacgtcgtag
     1621 gcgatgatcg tgaattcgcc gtagtcggcg gtgggaatct gggcctgtgt gatgcggctg
     1681 acgagcttct cactgacgcg gcgatgggcg atgagctgct cgatcgagat gatcttgagc
     1741 ttgtgcttgt cggcgatggc gtgcaattcg tcgcgcgtgg cccgctcgcc gcggtcgttg
     1801 aggatttcgc acaacactcc caccggcgcg aggcccgcca tgcggcacag atcgacggtc
     1861 gcctcggtat gaccggcccg ccgcagcacg cccccttcct tcgccaggag cggatggacg
     1921 tggccagggc ggacgaagtc atccgcggtg gtcgccccat cggcgatcgc ccgcacggtg
     1981 cggcagcgct cttcagccgt gatgcccgtc ttggccgagc agtggtcgat cggcgtcaga
     2041 aagctcgtcc ccaggggggc ggtattcagt tccacgaccg gcgtcagctc cagccgctgg
     2101 gcgacttccg gcagcattgg ggcgcagaat tgcccgcggc cgccgatgag gaagttgatc
     2161 gcttcgggcg tcgccttttc cgccgcgcaa atgaaatcgc cttcgttttc gcggtcttcc
     2221 gcatcgacga cgatgatcac cgcgccagcg gcgatggcat cgacggcgga ttggatggtg
     2281 gagaaggcgg gcattggttg gtagcgtgtc tggatagttc gatgcgttat tatagacgaa
     2341 cgacaagcat gtaggacgga cctcctggtc cgtccgacca ggctaacgga cggaccggag
     2401 gtccatccta cgggggcacc cgtgctcgaa cgcgaagaat acatcgaaca ggcccacctc
     2461 ttccgcgtgc tcggcgagcg gttggcggac aacgtgccga tgcaggagct gcttgaccat
     2521 gtgaagcagg aggtcctttc gaccacgcgg ctgccgatgg cgattgactt cctgctgacc
     2581 gagctgcggc acatcggcat gatgagcacg gcgatggagc ggctgccgca ctatttcacg
     2641 ccgttcagac gtacgtgatg caggaggcgg aggccgagca ggggcggttc gacctgcgaa
     2701 tcgccgtcgc cattctgcgg cacgaggcgg agtaccgggc gaagtcgccg attccgcagg
     2761 cgatgttcat gtatcagttc gagtgcctct gccgcaaccg gctgaagtat gacgcggggc
     2821 tggcggccgt cgccggcgat ccgatctacg acgagaactg gcagcagtgg attcgcgaac
     2881 tgcggctgcg gatcgggatg gtcgatttcg ccgacatgat ctacgttcgc agccagtaca
     2941 acctgatcca ccgcacacgc cccggcgaag agccgccgcc gcccgagaag cccgtgctct
     3001 tcggcgagaa ggagggaaaa attgcctggg cgaaccggcg gaaggacccg ctgtatctgt
     3061 tcgcggccct gcagcggcac c
//
LOCUS       DASUGB010000409         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_879909, whole genome shotgun sequence.
ACCESSION   DASUGB010000409 DASUGB010000000
VERSION     DASUGB010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879909"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1268)
                     /locus_tag="VFV87_07860"
     CDS             complement(72..1268)
                     /locus_tag="VFV87_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5103707.1"
                     /translation="MLTRQIAPILFLSLSLLLPAAAFAQATPGKNITFKKTVIDAVFR
                     SEGIAVGDFNKDGKLDIAAGSVWYAAPEWKLQITEEKAPEFDPLAYSRSFQTFADDLN
                     GDGWTDIIVVEFPGDVTSWLENPKKEGMLWPKHMLTRVTNNESPQYLDVDGDGKRDLL
                     AAFSPDPANVDGPQKRMVFMSRKQDPFEPWTILSVSAAGAPGTNRFSHGLGLGDINRD
                     GRDDVLVPQGWWEAPQDRRSGDWKFHPAKLGENCAQMYAFDFDGDGDNDVLSSAAHAV
                     GMWWHEQLEGGEWKTHLIDDSFTQTHALCLADINGDGLPDFVTGKRWWAHGPKGDINP
                     DHPAVMCWFELSREEGKPVWTKHQFDHDSGVGTQFEVADVNGDGLLDVVTSNKKGTFY
                     FEQVRE"
     gene            1418..2881
                     /gene="cls"
                     /locus_tag="VFV87_07865"
     CDS             1418..2881
                     /gene="cls"
                     /locus_tag="VFV87_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5103708.1"
                     /translation="MSQVLEFLLTYIWPLALAALHVVLMAVASCHVVLTKRDPRAALG
                     WVGIICLTPIFGTILYVMFGVNRIHRRARSLRRSRTDSEAVHEPRPVSDDVLVTVLGD
                     EATHLAPLSPLVGRLTRLPLLDGNRVTPLISGHAAYDAMLAEIDAAEHSVALMTYIFD
                     NDACGLRFADALERAQQRSVQVRVLIDDVGARYTIPSIRRTLKKRGIPNATFLPTLTP
                     VKLHYTNLRNHRKILVIDGKVGFTGGMNIRQGHLCDVNCKHPVLDLHFRIDGPVVDHL
                     RTAFAADWDFATQEHLDGTDWFPPLAPAGHSVCRGIIDGPEIESETLRLTILGAVGCA
                     RHSIQIVTPYFLPDQPLITALNVAAMRGVAVHIVLPEKGNLRLVQWASTAQLWQVLQR
                     GCRVWLTPPPFDHTKVMLVDGAWSLIGSGNWDPRSLRLNFEFNVECYDQELAAELAAL
                     IQKKKAGARETTLAEVDGRPLWMRLRDGFCRLAQPYL"
     gene            complement(2948..>3195)
                     /locus_tag="VFV87_07870"
     CDS             complement(2948..>3195)
                     /locus_tag="VFV87_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103709.1"
                     /translation="EPELLETQSENEEDCCDTPLAEALHSILGNTVSNLKSLASEGVE
                     NIELDDLADVVKSALDDLFARLACDDALGFDPFDHLS"
BASE COUNT          571 a         1021 c          955 g          648 t
ORIGIN      
        1 tgtcctctgt cctctgtcct ctgtccgccg tcccctgtcc ttctcctctg aaccctgaac
       61 cctcccccgc gctactcccg cacctgctca aagtagaatg tccccttttt gttggacgtg
      121 acgacgtcga gcaggccgtc gccgttgacg tcggccactt cgaattgcgt gccgacgccg
      181 ctgtcgtggt cgaactggtg cttcgtccag accggcttgc cttcttcgcg ggagagctcg
      241 aaccagcaca tcaccgcggg atggtcagga ttgatatcgc ccttggggcc gtgagcccac
      301 caccgcttgc ccgtgacgaa gtccggcagg ccgtcaccgt tgatgtcggc caggcacagg
      361 gcatgcgtct gcgtaaagct gtcgtcaatg agatgcgtct tccactcgcc gccctcaagt
      421 tgctcgtgcc accacatgcc aaccgcgtgg gcagcggacg agagcacgtc gttatcgccg
      481 tcgccgtcga agtcgaacgc gtacatctga gcgcagtttt cgccgagctt cgcggggtga
      541 aacttccagt cgcccgagcg gcggtcctgg ggggcttccc accagccctg cgggacgagc
      601 acgtcgtcac ggccgtcgcg attgatgtca cccagtccca aaccgtgcga aaaccggttc
      661 gtgcccggcg caccggcggc ggagacggac aaaatcgtcc agggctcgaa ggggtcttgt
      721 ttgcgcgaca tgaacaccat ccgcttctgc gggccatcca cgttggccgg atcgggcgaa
      781 aatgccgcga gcagatcgcg tttgccatcg ccgtccacat cgaggtattg cgggctctcg
      841 ttgttcgtga ctcgcgtgag catgtgcttg ggccagagca tgccttcctt cttagggttc
      901 tccagccagc ttgtcacgtc acccggaaac tccacgacga taatgtccgt ccagccgtcg
      961 ccgttcaggt cgtcggcaaa cgtctggaac gaacggctat aggcgagcgg gtcgaactcg
     1021 ggggccttct cttctgtgat ttgcagcttc cactccggcg ctgcatacca gacgctgccg
     1081 gcggcaatgt ccaacttccc gtccttgttg aaatcaccga ctgcaatccc ctcgctgcgg
     1141 aagaccgcgt cgatcaccgt ctttttgaag gtgatgttct ttccaggcgt cgcctgggca
     1201 aacgcggccg ccggaaggag aagcgacagg cttagaaata gaattggagc aatctggcgc
     1261 gtaagcatgc gatggaaccc ctgggtggca ttacttggaa aaccgatatt cttatcggcc
     1321 ctattctatc cccagcggat gggcaattaa cacaacctga aatcagaatt ccgcagcccc
     1381 cctggcctgc cacgagtggg gccgcgacga tgttcgcatg tcgcaagtgc tggaattctt
     1441 gttgacctat atctggccgc tggcgctggc cgccttgcac gtggtcctga tggcagtcgc
     1501 ttcgtgccat gtcgtgctga cgaagcgcga tccgcgggcg gcgctaggct gggtgggcat
     1561 catctgcctg acaccgatct tcgggacgat tctgtacgtc atgttcggcg tgaaccgaat
     1621 ccaccgccgc gcccgttcgc tgcgccgcag ccggacagac tccgaggcgg tgcatgagcc
     1681 taggcccgtt tcggacgatg tgctggtgac cgtcctcggc gacgaagcca cgcacttggc
     1741 gccactgtcg ccgctggtcg gcaggctgac cagattgccg ctcttggacg gcaaccgcgt
     1801 cacgcctttg atcagcggcc atgccgcgta cgacgcgatg ctcgcagaga tcgacgctgc
     1861 cgagcactcg gtcgccctga tgacctacat ctttgacaac gatgcgtgcg ggctccggtt
     1921 tgcggacgct ctggagcgcg cccagcaaag gagcgtgcag gttcgcgtgc tgatcgacga
     1981 cgtcggcgcc cgttacacga tcccgtccat tcgccggacg ctgaaaaagc ggggcatccc
     2041 caacgccaca tttctgccca cgctgacccc ggtcaagctc cactacacca atctccgcaa
     2101 tcaccgcaag attctggtga tcgacggcaa agtgggcttc accggtggca tgaacattcg
     2161 ccagggccat ctgtgcgatg tgaactgcaa gcatcccgta ttagacctcc acttccgcat
     2221 cgatggaccg gtggtcgatc acctgcgcac tgcctttgcc gccgattggg acttcgcaac
     2281 acaggaacac ctcgacggta ccgactggtt tccgccgctg gcacccgcag gtcattccgt
     2341 ctgcaggggg atcatcgacg gacccgaaat cgaatcggaa accctccgtt tgacgatttt
     2401 aggcgcagtc ggttgtgccc ggcattcgat tcagattgtc acgccttatt ttctgccaga
     2461 tcagccgctg ataacggccc tcaacgtggc cgcgatgcgg ggtgtcgccg tccacatcgt
     2521 gctgccggaa aaaggcaacc tcagactcgt gcaatgggcc agcacggccc agctttggca
     2581 ggtgctgcaa cgcggctgcc gcgtctggct cacgccgccg ccgttcgatc acacgaaggt
     2641 catgctcgtc gatggcgcct ggtcgctgat cgggtcggga aattgggacc cgcgcagcct
     2701 gcggctgaat ttcgaattca acgtcgagtg ctacgaccag gagttggccg ccgagttggc
     2761 cgccttgatc cagaagaaga aggccggggc acgagagacc acactggcgg aggtcgatgg
     2821 acggccgctg tggatgcgct tgcgagacgg cttctgccgg ctggcacagc cgtatttgta
     2881 gaacgcagcc gattggccca cggctgcgat gtccacgggc gacgcgctgc ttcgtccgcc
     2941 gaacgcgtca gctcaggtga tcgaacggat cgaagccgag tgcgtcgtcg caggcgaggc
     3001 gggcgaagag gtcatccagt gcggatttga ccacgtcggc cagatcatcg agttcaatgt
     3061 tctcgacgcc ttccgaggcc agcgatttca ggttgctcac ggtgttcccc aggatcgaat
     3121 ggagcgcttc cgcaagcggc gtgtcgcagc agtcctcctc attttcggat tgcgtttcaa
     3181 gcaactccgg ctcac
//
LOCUS       DASUGB010000410         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_45404,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000410 DASUGB010000000
VERSION     DASUGB010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45404"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFV87_07875"
     CDS             <1..669
                     /locus_tag="VFV87_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5103710.1"
                     /translation="NARWDNEASVLLGDFLFTHAFYLASTLDSVLGCRLIGRATNIVC
                     EGELRQKGSRGNFALTQAEYLAIIEAKTAELTAVSCRLGALFAGAAEDVVEQLDGFGR
                     DLGIAFQIADDLLDVLGEESATGKSLGTDLAKQKPTLPIIRALELASESDRAALLDLL
                     SGESCHSARLAPYLDRYEAVAYTRARAADFASRARQRLARLPSSPACDVLVSMTDFVI
                     SRSA"
     gene            complement(673..1518)
                     /locus_tag="VFV87_07880"
     CDS             complement(673..1518)
                     /locus_tag="VFV87_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103711.1"
                     /translation="MLRFSFPMLVWCGITLVAAAQESPDKEAAREARLKAMQQIAGRF
                     KVQAGEGAERKDVPLLEAPLLRFNDPARDFHDATIWGWGEEGRPLCLLTVEQYGERSW
                     FECISLAADPFAAEAETLRWSPKAAGVELQPYPDAPAVADKAPRRLIQMKELLGELRA
                     YEVGHDGSRYELRLMPKQLHRYDNAEAGLVDGAIFAFAYGTNPELLALIEARLPAEAA
                     PTADKLTADKPAAAQWQVGFARCGTAEPHVLLAEKEIFTLPYARGTKPGDAYWNFSYQ
                     FKKAE"
     gene            complement(1577..>2239)
                     /locus_tag="VFV87_07885"
     CDS             complement(1577..>2239)
                     /locus_tag="VFV87_07885"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5103712.1"
                     /translation="LDFAKSLKKKKIVEMLEAAVAKGGKTASKGDKGAKAKPAKVPSA
                     STSWDHLESCLQKSHPKVFQTLARPATARQVSELIVAVKIRITKEVQEFFHRHNGQTG
                     GPELIELDGEQYGFRLLSTEQAAREWSVWKELNDGGDFADAEAAADDTVRETWWHDKW
                     LPMAGNGAGDFLCFDFSPAHGGKQGQIITLWHERPNREVAFESVRHVLAELAAVWEAK
                     LP"
BASE COUNT          345 a          795 c          681 g          418 t
ORIGIN      
        1 aacgcccgct gggacaacga agccagcgtg ctgctgggcg atttcctctt cacccacgca
       61 ttctatctgg ccagcacgct cgacagcgtc ctcggctgcc ggctgattgg ccgggcgacg
      121 aacatcgtct gcgaaggcga gctgcggcag aaagggagcc gaggcaattt cgccctcacg
      181 caggccgaat acctggcgat catcgaagcc aaaacggccg agctgaccgc cgtgagctgc
      241 cgcctcggcg cgctcttcgc cggcgctgcc gaagatgtgg tggagcagct cgacggcttc
      301 gggcgagacc tgggaatcgc ctttcagatc gccgacgatc tcctcgacgt gctgggcgag
      361 gaatcggcca ccggcaaatc gctcggcacc gacctggcca agcaaaaacc aacgctgccg
      421 atcatccgcg cgctggagct cgcctccgaa tccgaccgcg ccgctctgct cgacctgctc
      481 agcggcgaat cttgccattc cgcgcggctg gccccatacc tcgaccgcta cgaagccgtc
      541 gcttacaccc gcgcccgcgc cgccgacttc gcgtcgcgcg cccgccagcg cctcgccaga
      601 ctcccttcca gccccgcctg cgacgtcctg gtctctatga ccgacttcgt catcagccgt
      661 tcggcgtaga ggttattccg ccttcttgaa ctgatacgag aaattccaat aggcatcgcc
      721 tggcttggtg ccgcgtgcgt agggtagcgt gaagatctcc ttctcggcga gcagcacgtg
      781 cggttcggcc gtgccgcagc gggcgaagcc gacttgccac tgggcggcgg ccggcttgtc
      841 ggcggttagc ttgtcggccg tgggggcggc ttcggctgga aggcgggctt cgatcagggc
      901 cagcagctcc gggttggtgc cgtacgcgaa ggcgaagatc gcgccatcca ccaggccggc
      961 ctccgcgttg tcgtagcggt ggagctgctt gggcatcagc cgcagctcat accggctgcc
     1021 gtcgtggccg acctcgtagg cccgcagctc gccgagcagc tccttcatct ggatcagccg
     1081 ccgcggggct ttgtcggcga cagcgggcgc atcgggatag ggctgcaact cgacgccggc
     1141 cgccttcggc gaccagcgga gcgtttccgc ttccgccgcg aatgggtcgg ctgcgagcga
     1201 aatgcactcg aaccacgacc gctcgccata ttgctcgacc gtcagcaggc acagcggccg
     1261 cccctcttcg ccccagcccc agatcgtcgc gtcatgaaaa tcgcgcgccg gatcgttgaa
     1321 ccgcagcagc ggcgcttcca acagcggtac gtccttccgc tccgcgccct cgcccgcctg
     1381 cactttgaac cgcccggcga tctgctgcat cgccttgagc cgcgcttcgc gcgcggcctc
     1441 tttgtcgggc gactcctgtg cggccgcaac caaggtgatt ccgcaccaga ccagcatcgg
     1501 aaaactgaat cgcagcatgg catcggctcc ccaggttgcc tgcattgtag cgatgcgaag
     1561 caccggctgc ggcggcctac ggaagctttg cctcccacac tgcggccagt tcggccagca
     1621 cgtgccgcac cgactcaaac gcgacctcgc ggttgggccg ctcgtgccac agcgtgatga
     1681 tttgtccttg cttgccccca tgggccgggc tgaagtcgaa gcacaggaaa tcccccgcgc
     1741 cgttgccggc catcggcagc catttgtcgt gccaccaggt ctcgcgcacc gtgtcatccg
     1801 ctgctgcttc ggcgtccgca aagtcgccgc cgtcgttgag ctctttccag acgctccatt
     1861 cccgggcggc ctgctccgtg ctcagcagcc ggaagccgta ctgctcgccg tccaactcga
     1921 tcagctcggg tccgcccgtc tggccgttgt ggcggtggaa gaactcctgg acctctttcg
     1981 tgatgcgaat cttgacggcg acaattagct cgctgacctg ccgggccgtt gcaggcctgg
     2041 ccagcgtctg gaagaccttg ggatgcgact tctgcaagca gctttcgagg tggtcccacg
     2101 aggtggatgc cgacggcact ttcgccggct ttgctttggc tcctttgtcc cccttggagg
     2161 ccgtcttgcc gcccttcgcg acggcagcct ccagcatctc cacgatcttt ttcttcttca
     2221 gactcttcgc aaaatccag
//
LOCUS       DASUGB010000411         5909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1991256, whole genome shotgun sequence.
ACCESSION   DASUGB010000411 DASUGB010000000
VERSION     DASUGB010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991256"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..798)
                     /gene="rplO"
                     /locus_tag="VFV87_07890"
     CDS             complement(292..798)
                     /gene="rplO"
                     /locus_tag="VFV87_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX5103713.1"
                     /translation="MSLHEVHKGVHKHKQPQRIGRGIGSGKGKTSGKGHKGQIARAGW
                     KALPIFQGGGSPLVRRVPKRGFTNSFALTVAEVNVGDLEELFNAGAEVTPEALENIGY
                     LKKRYDVLKVLGDGELKKKLTVSAHRFSASAKAKIEGAGGSVATLAAKKPASEKSPPK
                     KAPAKTKK"
     gene            complement(795..1283)
                     /gene="rpsE"
                     /locus_tag="VFV87_07895"
     CDS             complement(795..1283)
                     /gene="rpsE"
                     /locus_tag="VFV87_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5103714.1"
                     /translation="MATDTTRGEYIDKTVQIKRCAAVVKGGRRFSFAALVVVGDGKGR
                     VGWGYGKANEVPPAVEKGQKQATRRMVNVPLVGAHTIPHKVRAKFGSADVLLMPADAG
                     TGVIAGAAVRAVCEAAGIQNVITKSFNTSNQVTVVKAAIKALSQLRVQSDIERLRGVS
                     LS"
     gene            complement(1409..1777)
                     /gene="rplR"
                     /locus_tag="VFV87_07900"
     CDS             complement(1409..1777)
                     /gene="rplR"
                     /locus_tag="VFV87_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5103715.1"
                     /translation="MDKNKYLGGQRRRRRYRVRKRTRGSVDRPRLTVHRSHRNLDCQI
                     IDDATGKTLVAAGTREGDLRSSVKYGGNKAAAQAVGKAIAERALAAGIKQVCFDRGHY
                     LYHGRVAALADAARAAGLSF"
     gene            complement(1848..2393)
                     /gene="rplF"
                     /locus_tag="VFV87_07905"
     CDS             complement(1848..2393)
                     /gene="rplF"
                     /locus_tag="VFV87_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5103716.1"
                     /translation="MSRIGNKPIPLLDGVKVSVSGRQVNVEGPKGKLQWEHRREVAVK
                     VDPETKQVVVTRANDERPARAYHGLTRALISNMILGVKNGYEKKLEIIGVGYQAALAG
                     KTIKLRVGLANEIVRDIPAGLTVTCPDATHIVIQGCDKQQVGQFAAELRAARKPEPYK
                     GKGVRYQGEHVKIKPGKAATK"
     gene            complement(2480..2875)
                     /gene="rpsH"
                     /locus_tag="VFV87_07910"
     CDS             complement(2480..2875)
                     /gene="rpsH"
                     /locus_tag="VFV87_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5103717.1"
                     /translation="MLTDPIADMLTRIRNAIGVKRSHVDVPASQLKRGLAEVLKREGY
                     IWDWQEVEAEPINQLRLELKYGANGEEVIQHIKRISTPGRRVYRKAKEMRPVLNGLGI
                     TIISTSSGIVSDREARQKNIGGEVLCEVY"
     gene            complement(2892..3077)
                     /locus_tag="VFV87_07915"
     CDS             complement(2892..3077)
                     /locus_tag="VFV87_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX5103718.1"
                     /translation="MASKSKLAKAKQTPKFSTRAKRRCQLCGRPRAVYRKFGICRICF
                     RKLADQGLIPGVRKASW"
     gene            complement(3111..3671)
                     /gene="rplE"
                     /locus_tag="VFV87_07920"
     CDS             complement(3111..3671)
                     /gene="rplE"
                     /locus_tag="VFV87_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5103719.1"
                     /translation="MQERYFKQILPTLSEKVGRKNVLALPRLEKIVVNMGVGAATQEK
                     KHLEVAAEALGQITGQKPIITKARQAISAFRLREGMPIGCKVTLRGKRMYEFMDRLIS
                     IALPRVRDFRGISRRAFDGNGSYSLGLSEQLVFPELNPDKFTRVQGMNITFVVKNATD
                     DESRELLTQLGMPFQTEEPTEGKKKK"
     gene            complement(3930..4265)
                     /gene="rplX"
                     /locus_tag="VFV87_07925"
     CDS             complement(3930..4265)
                     /gene="rplX"
                     /locus_tag="VFV87_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5103720.1"
                     /translation="MYIKANDTVEVMTGDDKGQRGKVLSIDRRAGRLVVEGVNRVYKH
                     VRRSQKNPQGGRLSKEMPVQISNVLLLCNRCNRGVRIGARFQKDGAKERFCKKCGTSL
                     GVLAPPKKK"
     gene            complement(4265..4633)
                     /gene="rplN"
                     /locus_tag="VFV87_07930"
     CDS             complement(4265..4633)
                     /gene="rplN"
                     /locus_tag="VFV87_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5103721.1"
                     /translation="MIQQETRMAVADNTGAKEVMCVKVLGGSKRRTAGLGDVIICSVK
                     SVIAGSEIKKKAVVRAVVVRTKTPTRRKDGSYVKFDANAVVLIDAENNPRGTRIFGAV
                     ARELRERNFMKIVSLASEVV"
     gene            complement(4630..4935)
                     /gene="rpsQ"
                     /locus_tag="VFV87_07935"
     CDS             complement(4630..4935)
                     /gene="rpsQ"
                     /locus_tag="VFV87_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5103722.1"
                     /translation="MPKRLAIGVVTRDKAAKTRRVEIPRLVRHPMYGKILRRRTVCYA
                     HDEENVSKIGDTVEIEEAPPSSKLKRWVLKRVVSKAKQAGLEVADVEGATESSSGAT"
     gene            complement(4982..5179)
                     /gene="rpmC"
                     /locus_tag="VFV87_07940"
     CDS             complement(4982..5179)
                     /gene="rpmC"
                     /locus_tag="VFV87_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX5103723.1"
                     /translation="MKTSDLREMSDEQLQLTLKETENNLFRLRMQAQTERLDAPSELR
                     RHRRLIARIKTVQRQRQPAAT"
     gene            complement(5197..5613)
                     /gene="rplP"
                     /locus_tag="VFV87_07945"
     CDS             complement(5197..5613)
                     /gene="rplP"
                     /locus_tag="VFV87_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5103724.1"
                     /translation="MALMPKRVKHRKSQRGRIKGDATRGNRVVFGDYGLQATQGGWIR
                     AQTIESGRIAAQQYIRGEGRLYIRMFPHKSITARPLETRMGTGKGEPAAWVAVIKRGT
                     VLFELGGVSEAQAKLCFARLAHKMPVRVRMIKRRPA"
     gene            complement(5552..>5909)
                     /gene="rpsC"
                     /locus_tag="VFV87_07950"
     CDS             complement(5552..>5909)
                     /gene="rpsC"
                     /locus_tag="VFV87_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5103725.1"
                     /translation="LVAEDIAQQLGKRASFRRTMKRAIETTMDSGAKGIKIQLAGRLG
                     GSEMARCEKATAGSLPLSTLRAKIDYGFAEAWTAQGNLGVQVWINQGSFEGDATDGAD
                     AQAGEAPKKPKRTYKR"
BASE COUNT          911 a         1876 c         1762 g         1360 t
ORIGIN      
        1 tgttctttgc aagctgggcc tggtcgatga tcggcagcgg gatgtgatag ccgatgcgaa
       61 acacggccag gagcagaagc gtgaagaaga tcttctgccg caactccgga atggtgaaaa
      121 tcgcgcggag tttttccaac atctgattgc ccgtcctgga ttgactgggg gcgaaccggc
      181 cacagccgaa gcctgccgtt acagtcgccg tgtctcgcct tgcttcacca aattaccaca
      241 agcaccgaaa tccggccatg caggtttttc gccggatcct gcagtccctt gttatttctt
      301 ggttttggct ggagcttttt tggggggtga cttctcgctg gcgggtttct tggccgcaag
      361 tgtcgcgact gaaccgcccg cgccttcgat cttcgccttg gctgaggcac taaagcggtg
      421 ggccgaaacc gtcagctttt tcttgagctc accgtcgccc aggaccttca gcacgtcgta
      481 gcgttttttc agatagccga tgttttcgag cgcttcgggg gtgacttcgg cgccggcgtt
      541 gaacagttct tccaggtcgc ccacattgac ttcagccacc gtgagggcga acgaattggt
      601 aaaaccccgc ttgggaacgc gccgcaccag cggcgaaccg ccaccctgaa agatcggcag
      661 cgccttccag ccggcccggg cgatttggcc cttgtgccct ttgccggagg tcttgccctt
      721 ccccgagcca atgccgcggc caattcgctg cggctgtttg tgcttgtgaa cgcctttgtg
      781 aacttcgtgc aggctcatga cagcgaaact ccccgcagcc gctcaatatc cgactgcacg
      841 cgcaactggc tgagggcctt gatggcggct ttgacgaccg tcacctggtt gctggtgttg
      901 aagcttttgg taatcacatt ctggatgccg gcggcttcgc agacggcccg gaccgcggcg
      961 ccagcgatga cgcccgtgcc ggcgtcggcc ggcatgagca gcacgtcggc cgagccgaat
     1021 ttggcccgca ctttgtgcgg aatggtgtga gcgccgacca gcggcacgtt caccatccgg
     1081 cgcgtggcct gcttttggcc cttctcgacc gccggtggca cttcgttcgc cttgccgtag
     1141 ccccagccga cacggccttt gccgtcaccg acgacgacca gcgccgcaaa gctgaaccgg
     1201 cgtccgcctt tgaccaccgc ggcgcagcgc ttgatctgca ccgtcttgtc gatgtattcc
     1261 ccgcgtgtgg tatcggttgc cacagtcaac tccaggttga agttccaaga accgaagaac
     1321 cgaagaacca aattccggtt ccggcgccac agctctcaaa acttgacttc attggatctt
     1381 ggttcttacg ttcttggttc ttctgttgct aaaagctcaa ccccgccgca cgagccgcgt
     1441 ccgccagggc cgcgacacgc ccgtgataca aatagtgtcc gcggtcaaag cacacctgct
     1501 tgatgccggc ggccagggcc cgctcggcaa ttgccttgcc gaccgcttgc gccgcggcct
     1561 tgtttccgcc gtacttgacg ctcgagcgca ggtcaccttc ccgggtcccc gcggccacca
     1621 gcgtcttgcc ggtggcatcg tcgatgatct ggcaatccag attgcggtgg ctgcggtgca
     1681 ctgtcagccg cggacgatcg acagacccgc gcgttcgctt ccgcacgcga taccgccgcc
     1741 gccgccgttg tccgcccaag tacttgttct tatccacggc taaatcacct tggctggcaa
     1801 atgcctctcc gcaaatagga gagacgaccg agcaatcttc agttcaatta tttcgtagcg
     1861 gccttgccgg gcttgatctt cacgtgttcg ccctggtaac gcacgccctt gcccttatag
     1921 ggctcaggct tgcgggccgc ccggagctcg gctgcgaact ggcctacttg ctgcttgtcg
     1981 cagccttgaa tcacgatgtg cgtggcgtcc gggcaggtta cggtcaaacc cgcgggaatg
     2041 tcccgcacga tctcattggc cagaccgacg cggagcttga tcgtcttgcc ggccagggcg
     2101 gcctgatacc caacgccgat gatctccaac tttttttcgt agccgttctt cacgcccagg
     2161 atcatgttgc tgatcagggc gcgagtcagg ccgtggtagg cccgggccgg ccgctcgtca
     2221 ttggcacgtg tgaccacgac ttgcttcgtc tccggatcaa ccttcacggc gacctcgcgg
     2281 cgatgctccc actggagctt gcccttcggt ccttcgacgt tgacctgccg accggagacg
     2341 gagactttga cgccgtcgag caggggaatg ggtttgttgc cgatgcggga catttcaact
     2401 tgtcacttgt catttgtcac ttgtcatttg tgtttgatcg ctcgtgcaaa ggaccaatga
     2461 caaatgacca atgacttcgt tagtaaacct cacacaatac ttcgccgcca atgttcttct
     2521 gtcgggcctc gcggtcgctc acgatgccgc tggacgtgct gatgatcgta atgcccagac
     2581 cattgaggac cgggcgcatt tctttggcct tgcgatacac gcggcggccg ggggtgctga
     2641 tccgtttgat gtgctgaatc acttcttcgc cgttggcgcc gtacttgagc tccagccgca
     2701 attggttgat gggctccgct tcgacttcct gccagtccca aatgtagcct tcgcgcttga
     2761 ggacctcggc gaggccccgc ttgagctggg acgcgggcac atccacgtgc gatcgcttga
     2821 cgccgatggc gttgcggatg cgggtcaaca tatcggcgat gggatcggtc aacataggca
     2881 gcaatttccc cttaccaact ggccttgcgc acgccgggaa tcaatccctg atcggcgagc
     2941 ttgcggaaac aaatccggca gatgccgaac ttccggtaca cggcgcgcgg acggccgcaa
     3001 agctgacacc gccgtttggc acgggtcgag aacttgggcg tctgtttcgc cttcgcgagt
     3061 ttggatttac tggccacaac gaattctcag cagttggtcc ggtgaggtgg ttatttcttc
     3121 ttcttgccct cggtgggctc ttcggtctgg aacggcatgc cgagctgcgt cagcaactcg
     3181 cgcgattcgt catcggtcgc gttcttcacg acgaacgtga tgttcattcc ctggacgcga
     3241 gtgaacttgt ccgggttcag ttccgggaac acgagttgct cggaaaggcc caggctgtag
     3301 ctgccgttgc cgtcgaacgc tctgcggctg atgccgcgga agtcgcggac gcgcggcagg
     3361 gcgatcgaaa tcagccggtc catgaactcg tacatccgct tgccgcggag cgtcaccttg
     3421 catccgattg gcatcccctc ccgcaggcgg aaggcagaaa tggcctggcg ggccttggtg
     3481 atgatcggct tctggccagt gatctgtccc agagcttcgg cggcgacttc caggtgcttc
     3541 ttttcctgcg tcgccgcgcc gacgcccatg ttgacgacga tcttctccaa ccgcggcagc
     3601 gcgagaacgt ttttgcggcc caccttctca gagagcgtgg gcaggatctg cttgaaatag
     3661 cgctcctgca gacgcggcgt ggggacgggg ccttgcggct cggcctcggc gcctttcttg
     3721 cccttctcgc ctttgggggc cttgtcgcct ttgggggcct tgtcgccttt ggcgggcttg
     3781 tcgcctttgg cgggcttgtc ccccttggcg ggcttttcgg gcttctcagc cttttcgccc
     3841 ttgggcggct tggccggctt atcggcttcg gtagtatctt ggtctttggc catggctgtg
     3901 gtcaggtcgg cgggaccttt tgcacgaaac tatttcttct tgggtggggc gagcactccg
     3961 agcgacgttc cgcacttctt gcagaatcgc tctttggcgc cgtctttctg gaaccgtgcc
     4021 ccgatccgca cgccgcggtt gcagcgattg cagagcagca gcacattgct gatctggaca
     4081 ggcatttcct tggagagacg gccaccctgc ggatttttct ggctgcggcg gacgtgcttg
     4141 tagacgcggt tgacgccctc gacgaccagg cggcccgcgc gtcgatcgat cgacaacact
     4201 ttgccgcgct gccccttgtc gtcgccggtc atcacttcga ccgtatcatt ggctttgatg
     4261 tacatcacac aacctcgctc gccaggctga cgatcttcat aaaattccgc tcgcgcagct
     4321 cgcgggcgac ggcgccgaag atgcgcgtgc cgcgaggatt gttctcggcg tcgatcagca
     4381 cgacggcgtt cgcgtcgaat ttgacgtagc tgccgtcctt gcggcgggtg ggggtcttgg
     4441 tgcggacgac gaccgcccgc acgaccgcct tcttcttgat ttcgctgccg gcgatgacgc
     4501 tcttgacgct gcaaataatc acgtcgccca ggcccgcggt gcggcgcttg gagccaccca
     4561 gcaccttgac gcacatcact tccttggcgc cggtgttatc ggcaaccgcc atgcgtgttt
     4621 cttgctggat catgttgcac cactggatga ttcggttgcg ccttccacat ccgccacctc
     4681 gagcccggct tgcttcgcct tgctgacgac gcgcttgagg acccagcgct tgagcttgga
     4741 gctcggcggg gcttcctcga tttcgacggt gtcgccaatc ttggagacgt tttcctcatc
     4801 gtgggcgtag cagacggtgc ggcggcgcag gatcttccca tacatgggat ggcgcaccag
     4861 ccgcggaatc tccacgcggc gggttttggc cgctttgtcg cgtgtcacca cgccgattgc
     4921 aagtcgcttg ggcataaccg ggctcgtgtc gttggttctt ggttcttgtc tcttggttct
     4981 tctacgtggc cgccggctgc cgctggcgct gcaccgtctt gatgcgggcg atcaaccgcc
     5041 ggtgacgccg cagttcgctc ggcgcatcca gccgctcggt ctgcgcctgc atccgcaggc
     5101 ggaacaggtt gttctccgtt tccttgagcg tgagctgcag ttgctcgtcg ctcatttctc
     5161 gtagatcact ggtcttcatg gctcgttctc ttccgactat gcgggtctcc gcttgatcat
     5221 ccgtacgcgc accggcatct tgtgcgccaa gcgggcgaaa cacagcttgg cctgggcctc
     5281 gctgacgccg cccaattcaa acagcacggt gccgcgcttg atgacggcga cccacgctgc
     5341 gggctcgccc ttgcccgttc ccatgcgggt ttcgagcggt cgggcggtga tcgacttgtg
     5401 cgggaacatg cggatgtaca gccgaccctc gccgcggatg tattgctggg ccgcaatacg
     5461 tccggactcg atggtctggg cccttatcca cccgccctgg gttgcctgaa ggccgtagtc
     5521 gccgaatacc acgcggttgc cccgcgtggc atcacctttt atacgtcctc tttggctttt
     5581 tcggtgcttc acccgcttgg gcatcagcgc catcagtagc gtctccctcg aacgaacctt
     5641 gattgatcca cacttgcacg cccaggtttc cctgagccgt ccacgcctcc gcgaagccgt
     5701 agtcgatctt cgcccggagc gtggaaagcg gcagcgagcc ggccgtggcc ttctcgcacc
     5761 gcgccatttc cgagccgcca aggcggccgg cgagttgtat cttgatgccc ttggccccgg
     5821 agtccatcgt cgtttcgatg gctcgcttca tcgtgcggcg gaagctggcc cgcttgccga
     5881 gctgctgggc gatgtcttcc gccacgagc
//
LOCUS       DASUGB010000412         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_185098, whole genome shotgun sequence.
ACCESSION   DASUGB010000412 DASUGB010000000
VERSION     DASUGB010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185098"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFV87_07955"
     CDS             complement(<1..478)
                     /locus_tag="VFV87_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103726.1"
                     /translation="MQRRHFLAAGAATLAAASGPFWLRFHSSSVQQRASQPPAFSIIP
                     VVGDGKWIWTEPPKETGYLEPRPHELSIGVELQGEGDASQVRASTPVPIQWPEQSIED
                     VRIETDGCEARIQQVGEGAAQLLLAAPGMRRGQTVRAIAHYKLTLKKQHLGHQLEHF"
     gene            complement(604..1857)
                     /locus_tag="VFV87_07960"
     CDS             complement(604..1857)
                     /locus_tag="VFV87_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5103727.1"
                     /translation="MLISNGQLIDGTGAAPVPKAALVIEEGRVAYAGPVGEMPTVPAS
                     AWRIDARGGTIMPGLVEAHYHPTYFNVAALEDLDIKYPVEYVTLLAACNAKLALECGY
                     TAARSGGSLFNIDVWLKKAIENDLIPGPRLAASGREICGVGGLMDWNPDYRKIGMEGL
                     VLLINGPDEARAAVRKLVKDGIEWVKTYPTGDAAAPDANDHHTLCMTPGEMHAVVQTA
                     HNHNLKVTGHCRATEGIKNALHAGYDALEHGTFMDDEALDMLLARDVPVVPALYFELA
                     SIERGPEFGMSQRVIDGHKETLEGGAESARKILKAGGRLGMGGDYGFAWNPHGDYARE
                     LSFFVNYVGFTPLETIKCATKTGAEILGRGDEIGTLEAGKLADVLVVDGDVAADISLL
                     QRRERFIAVMQGGVVKAGQFLAPSA"
     gene            1976..>2439
                     /locus_tag="VFV87_07965"
     CDS             1976..>2439
                     /locus_tag="VFV87_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103728.1"
                     /translation="MTTPPFRVGVTRDFLKPDGSLGFGDIGLSLLDDQPGIAWEFLPD
                     DYLEMPAEIADQYDALMVLGPRIARQTVAGSKRFALVARFGVGYDSIDVPACTENGVL
                     LTITPGGVRRPVATSALAFLLALTHKMFVKDRITREGRWAERLDHNGVGLVGR"
BASE COUNT          385 a          817 c          768 g          469 t
ORIGIN      
        1 gaaaatgttc cagctggtgg ccgagatgct gctttttcag cgttagtttg taatgtgcga
       61 ttgcccgcac cgtttgcccg cggcgcatgc ccggcgcggc gaggagcagt tgggcggcgc
      121 cttcgcccac ctgctgaatc cgggcttcgc agccgtcggt ttcgatccgc acgtcctcga
      181 tcgactgctc gggccactgg atgggcacgg gcgtgctggc acggacttgg ctggcatccc
      241 cttcgccctg caattcgacg ccaatggaca gctcgtgcgg acggggctcc aaatagcccg
      301 tctctttggg cggctcggtc cagatccact tgccgtcgcc gacgaccgga atgatcgaga
      361 acgccggcgg ctgcgaggcg cgctgctgca cggaggaaga atggaatcgg agccaaaacg
      421 gcccgctggc ggccgcgagc gtcgcggcgc cggcggccag gaaatgccgc cgctgcatga
      481 tggccctccg tcgtgccggc agcgggtgaa ctgaaccaaa accgctgaca gcgatctgcc
      541 cctgctgcga agtgtacgca ttttggcgtg gcatgcaagc gatttggggc cgtttacctg
      601 cattcacgcc gacggcgcga gaaactgtcc ggctttcacg acgccgcctt gcatcacggc
      661 aatgaaccgt tcgcgccgct ggagcagcga aatatccgcc gccacgtcac catcgacgac
      721 gaggacgtcg gccaatttgc cggcttccag cgtgccaatt tcatcgccgc ggccaaggat
      781 ctcagctccg gtcttcgtgg cgcacttgat cgtctccagc ggcgtgaagc caacgtaatt
      841 cacgaaaaac gacagctcgc gggcataatc gccatgcgga ttccacgcaa agccgtaatc
      901 gccccccatg cccagccgtc cgccggcctt aagaatcttg cgggccgatt cggcgccccc
      961 ttcgagcgtt tccttgtggc cgtcgatcac ccgctgggac atgccgaact ccggcccgcg
     1021 ctcgatgctc gccagctcga agtagagcgc ggggacgacg ggcacgtcgc gcgcgagcag
     1081 catgtcgagc gcctcgtcgt ccatgaacgt gccgtgctcg agggcgtcgt agccggcgtg
     1141 gagggcgttc ttgatccctt cggtcgcccg gcagtggccg gtgaccttga ggttgtgatt
     1201 gtgggcggtc tgcacgacag cgtgcatttc gcccggcgtc atgcagagcg tgtggtgatc
     1261 gttggcgtcg ggcgcggcgg catcgcccgt gggatacgtc tttacccatt cgattccgtc
     1321 cttgaccagc ttgcggaccg cggcacgggc ttcgtccggc ccattgatca gcagcaccag
     1381 cccttccatg ccgatcttgc gatagtcggg gttccaatcc atcagtccgc cgacaccgca
     1441 gatttcccgc ccgctggccg ccaggcgcgg gccggggatc aggtcgttct cgatcgcctt
     1501 cttgagccag acatcgatgt tgaacaggct gccgccgctg cgggccgcgg tgtagccgca
     1561 ttcgagggcc agtttcgcat tgcacgccgc cagcagcgtg acgtattcga ccgggtattt
     1621 gatgtcgagg tcttccaggg ccgcgacgtt gaagtacgtc gggtgatagt gggcctcgac
     1681 gagccccggc atgatcgttc cgccgcgggc atcgatccgc caggcgctag ccgggaccgt
     1741 cggcatctcg ccgacaggtc cagcgtacgc gacgcgtccc tcctcgatca ccagggctgc
     1801 cttgggcaca ggtgccgcgc ccgtgccgtc gatgagctgc ccgttcgaaa tgagcattgt
     1861 gcccgttgcc gttttcatca tgctctcgct ctcgcttccc gcttggtagg agcggtctgc
     1921 caatctcgct acaatcttag gctctctcac gtcttccaca acctttcgca cacgaatgac
     1981 cactccgccc ttccgtgtcg gcgtcacccg cgattttctc aagcccgatg gctcgctcgg
     2041 ctttggcgat atcggcctgt cgctactgga cgaccagcca gggatcgcgt gggaattcct
     2101 gccggacgat tacctcgaaa tgccggccga aatcgccgac cagtacgacg cgctcatggt
     2161 cctcggcccg cgaatcgcac ggcaaacggt cgcgggctcg aagcgcttcg ccctcgtcgc
     2221 ccggttcggc gtgggttacg acagcatcga cgtgcccgcc tgcaccgaga atggcgtgct
     2281 gctgacgatt acgcccggcg gcgtgcggcg gccggtcgcc acctccgcgc tcgcgttcct
     2341 tctggctctc acccacaaaa tgttcgtcaa ggatcgcatc acccgcgaag gacgctgggc
     2401 cgagcggctg gaccacaacg gcgtggggct cgtggggcg
//
LOCUS       DASUGB010000413         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_324130, whole genome shotgun sequence.
ACCESSION   DASUGB010000413 DASUGB010000000
VERSION     DASUGB010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324130"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..695)
                     /locus_tag="VFV87_07970"
     CDS             complement(153..695)
                     /locus_tag="VFV87_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX5103729.1"
                     /translation="MFWPFRSSFLAQTAYGGIDGFLPWGRGSLMLDVVFVAMFVVVPV
                     LAASIYLVKARGRYALHKKLQLTLAAVLLVAVLMFEIDMQFITKWETRAEPSPYFAEA
                     NKWTCPAGVSLIVHLSFAIPTLLLWIYVVVQALRNFSRPPAPGPHSTNHARFGWAAAI
                     GMFLTAATGWVFYWMAFVAV"
     gene            899..1378
                     /locus_tag="VFV87_07975"
     CDS             899..1378
                     /locus_tag="VFV87_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103730.1"
                     /translation="MSSEYQPANPYLSPTYTPAPQQRERAPASLVVAAMAMIAAAGLG
                     LAFSGFNFALSFGEAVVDPSAPPMVQEFQRGTVGPVATGMQGGFMMLNLFIIICGVQM
                     MKMQSWGLSVTGAALSILNIGSCCCVLGLPIGIWSLSVLFSPEVISMFNTNNGQQQV"
     gene            complement(1399..>3796)
                     /locus_tag="VFV87_07980"
     CDS             complement(1399..>3796)
                     /locus_tag="VFV87_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX5103731.1"
                     /translation="DAPPNSKALPKLNIAQELGLAGDEGGTADHVSFSGAFRCQENTR
                     KGSLSLTATIEPGWHVYSVTQLKGGPMASKIKVAESPDFKVLGPFQPDRPPHIQQLEI
                     YKVPVEEHEGTVTWSAPIEIAEGVDPQSLTIGLTYSGQVCSDVCIPIFNQQALAKFAG
                     FTPAPEAVGEYRPDPLQSEVFIRGHLEPAAAKPGSTVNLVITATPNPGWHIYAYAPLD
                     PNEVAKPTLIHVVLPTGWKQGEVQASSDPIVKLGNKAVPTTRYYDQAVTWRVPITIPA
                     DAETGERVLTGYLGMQTCTDKNCRFPDAVEFRVSLPVAGQEQTGEIPLEFLPLKRETR
                     GAETAAVRGYADVAKLAAASTPANAESPAQTTPTTKPVDLNGLLIAAAIGFLGGIILN
                     FMPCVLPVIGLKILSFAQQGGQHRGRIFALNLWFFFGIMAVFMALATLAAVVNLFWGE
                     QFTYTWFKVALILVTFAMALSFLGVWEIPIPGFSQSMGKPEDAKEEGLSGAFLKGIFT
                     TLLATPCSGPLLGPLFTFVAGSSPIVAYVLFGSIGLGMGMPYLVIGAAPRLVRWLPKP
                     GAWMETFKQLMGFVLLFTVVYLFWSVPSYYVPTLAAMIGVWLACWWIGRVPVYEETNK
                     QVWAWIGGCAAAVLVGMIAFTMFGPKSEAEKLESIAWLPYSEEQLAQEISAGKTVMID
                     FTANWCPTCQLNTHFAIETPKIKALLEKNGVVPMLADWTEHSPEIKAKLQELKSASIP
                     FLAIYPAGQPAEVIRLQDVISESQLAQALEKAGPSKTAATASTGAVTAEALAGNQPSG
                     "
BASE COUNT          711 a         1231 c         1158 g          696 t
ORIGIN      
        1 aagagcaaga gccgctgccg ccgcccgact tcagcaagct ggcggagtga gagaccctcg
       61 cttgagtcgt ttaacggcca gccgaaggcg acgccgtctc tctgagcgtc cacaacgcgg
      121 acgccttcgg ctacgcgtta aacgagttcc ggctagaccg ccacgaaggc catccagtaa
      181 aacacccagc cggtcgcggc ggtcaaaaac atgccgatcg ccgcggccca gccgaagcgg
      241 gcgtgatttg tgctgtgcgg ccccggcgcg gggggccggc tgaagttccg cagggcctgc
      301 acgacgacgt aaatccacag gaggagcgtg ggaatcgcga acgacagatg cacgatcagg
      361 ctgacgccag ccgggcaagt ccacttgttg gcttcggcga agtacggcga cggctcggcc
      421 cgcgtttccc atttggtgat gaactgcatg tcgatctcga acatcagcac cgctaccagc
      481 aggaccgcgg cgagcgtcag ttgcagtttc ttgtgcagag cgtaccgtcc gcgggctttg
      541 accaaataaa tgctcgccgc cagcaccggc acgacgacga acatcgccac gaagacgacg
      601 tcgagcatca acgacccgcg gccccagggc agaaagccgt caatgccgcc ataggcggtt
      661 tgcgcgagga acgatgatcg gaaaggccaa aacatgcgag atacccgcgg cattgtagcg
      721 gagcatcgcc tggaggcgat gggcgcgtgg cgtagttcat cctagcgaac gtagcgattg
      781 tagcggcagc gcacgcgaac gattgatggg aatcgcaacc tatgcttggc tgtccgcgcg
      841 cgggacgcgg aaccctcact ccgcacgcac ggaccacctg ttgtaaggcg tccttcccat
      901 gagcagcgaa taccagcccg cgaatcccta tctcagcccc acctataccc cggccccgca
      961 gcaacgcgaa cgggcgccgg cttcgctggt ggtggcggcc atggcgatga ttgccgcggc
     1021 cgggctcgga ctggcgtttt cggggttcaa cttcgccttg tccttcggcg aggcggtggt
     1081 cgatccatca gccccgccga tggtccagga atttcagcgg ggaacggtcg gccccgtcgc
     1141 tacgggcatg caaggcggct tcatgatgct caacctgttc atcatcattt gcggcgtgca
     1201 gatgatgaaa atgcagagct ggggactctc ggtcaccggc gccgccctct cgatcctcaa
     1261 catcggcagc tgctgctgcg tgctcggatt gccaattggt atctggagcc tctcggtgct
     1321 gttctcgccc gaggtcatca gcatgttcaa caccaacaac ggccagcagc aggtctaaca
     1381 tgcccggcgg ccaatcgcct atcccgaagg ctgattgccc gccagcgcct cggccgtcac
     1441 cgcgccggtg ctcgccgtcg ctgccgtctt tgatggcccg gccttttcca gggcttgggc
     1501 gagctgcgac tcggagatca cgtcctgcag acggatgact tcggcgggct gtcctgcggg
     1561 atagatcgcc aggaagggaa ttgaggcgct cttgagctcc tgcagcttgg ccttgatctc
     1621 ggggctgtgc tcggtccaat cggcgagcat cggcacaacg ccgttcttct ccaacagcgc
     1681 cttgatcttc ggcgtctcga tggcaaagtg cgtgttgagc tggcacgtcg ggcaccagtt
     1741 cgctgtgaag tcgatcatca cggtcttgcc cgcactgatc tcttgtgcga gctgctcctc
     1801 ggaatacggc agccaggcga ttgattccag tttctctgcc tcagacttcg gcccgaacat
     1861 cgtgaacgcg atcattccca ccagcaccgc cgccgcacag ccgccgatcc acgcccacac
     1921 ctgtttgttc gtttcctcat acaccggcac gcgtccgatc caccagcacg ccagccatac
     1981 gccgatcatc gcggccagcg tcggcacgta gtagctcggc accgaccaga acagatacac
     2041 gaccgtaaac agcagcacga agcccatgag ctgcttgaag gtctccatcc acgcgccggg
     2101 tttcggcagc cagcgcacca gccgcggcgc ggcgccaatc accagatagg gcatccccat
     2161 tcccaggccg atgctgccga acagcacata tgccacgatc ggcgaactgc cggcgacgaa
     2221 cgtgaatagc ggccccaaga gcggcccgct gcacggcgtg gccagcagcg tcgtgaagat
     2281 gcccttgaga aacgccccgc tcagcccttc ttctttggca tcctccggct tgcccatcga
     2341 ctgcgagaag ccggggatcg gaatctccca cacgcccagg aagctgagcg ccattgcgaa
     2401 cgtgacgaga atcagcgcga ccttgaacca ggtgtacgtg aattgctcgc cccagaacag
     2461 gttgacgacc gcggccagcg tcgccagggc catgaacacg gccatgatcc cgaagaagaa
     2521 ccacaggttg agcgcgaaaa tccgcccgcg atgctggccg ccctgctgcg caaacgaaag
     2581 gatcttcagg ccgatcactg gcagcacgca gggcatgaaa ttcaaaatga ttccgcccaa
     2641 aaagccaatt gccgcggcaa tgagcaggcc gttcagatcg acgggcttgg tggtcggagt
     2701 cgtttgcgcg gggctttctg cattcgccgg cgtgctggct gctgcgagtt tggcgacgtc
     2761 ggcgtaaccc ctcacggcgg cagtctcggc accacgagtt tctcgcttaa gcggcaagaa
     2821 ttccaaggga atctcgcccg tctgttcttg cccggccacc ggcaggctga cgcggaactc
     2881 gaccgcgtct ggaaatcggc agttcttgtc ggtacacgtc tgcattccca ggtagccggt
     2941 gagcacccgt tcgcccgttt ctgcatcggc ggggatcgtg atcggcactc tccacgttac
     3001 cgcctggtca taatagcgcg tcgtaggcac cgccttgttg ccaagcttca cgatcggatc
     3061 gctcgaggcc tgcacctctc cttgtttcca gcccgtaggc aacacgacgt gaatcagcgt
     3121 cggcttggcg acctcgttgg gatcgagcgg cgcgtaggca tagatgtgcc agcccggatt
     3181 gggtgtcgct gtgatgacga gatttacggt gctgccgggt ttcgctgctg ccggttccag
     3241 atgcccgcga atgaagacct ccgactgcag cggatccggc cggtactcgc ccaccgcttc
     3301 gggcgcgggg gtgaagccgg cgaatttggc cagggcctgt tggttgaaga tcgggatgca
     3361 aacgtccgag cagacctgcc cgctgtaggt caggccgatg gtgagcgact gcggatcgac
     3421 cccttcggcg atctcgatcg gtgcgctcca tgtcaccgtt ccctcgtgct cttccaccgg
     3481 gaccttgtaa atctcaagct gctggatgtg cggcgggcgg tccggctgaa acgggccaag
     3541 gaccttgaag tcaggcgact cggcgacctt gatcttcgac gccatcggcc cgcctttgag
     3601 ttgcgtgacc gaatagacgt gccagcccgg ctcaatcgtc gccgtcaggc tgagcgaccc
     3661 tttccgtgtg ttttcctggc agcggaacgc cccggaaaac gacacgtggt ccgccgtgcc
     3721 cccctcatcg cccgccaggc ccagctcctg ggcgatgttg agcttcggca gagccttgga
     3781 attgggtggc gcgtcc
//
LOCUS       DASUGB010000414         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1991442, whole genome shotgun sequence.
ACCESSION   DASUGB010000414 DASUGB010000000
VERSION     DASUGB010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991442"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFV87_07985"
     CDS             complement(<1..622)
                     /locus_tag="VFV87_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5103732.1"
                     /translation="MKSEMLVNVSQPEECRIAIIEDGVLEELYIERASQESFVGNIYK
                     GRIVNLEPSIQAAFVDFGVGRNGFLHISDVEPQYFRQGGFDPSEPMGGGRLADIDVGD
                     DDPPPRAGDRGDERGHDRGNDRGRGNDRGRGNDRGNGGRYRRTNRGGRPRIKPPIQEV
                     FRRGDEVLVQVIKEGIGTKGPTLSTYISIPGRYLVLMPALGRVGVSR"
     gene            complement(1023..1319)
                     /locus_tag="VFV87_07990"
     CDS             complement(1023..1319)
                     /locus_tag="VFV87_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX5103733.1"
                     /translation="MEPTAHKAVTLQNPQGFHARPAHLFVKLASQFQSQIEILKGNEV
                     INGKNILDLLTMGAGNGTTLTLRAIGPDAENAVNALSQLVEGGFGEMGPTAPVP"
     gene            complement(1408..1884)
                     /locus_tag="VFV87_07995"
     CDS             complement(1408..1884)
                     /locus_tag="VFV87_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5103734.1"
                     /translation="MRFSDFISVAAIRADLATDEKPAIIRELVESLAAAGAVKPADVE
                     GIVAAIMKREELGSTGIGRGVAVPHTKHASVEKLVGTVGVSVEGVDFNSLDGDKVNLF
                     FLLVSPPDRPGDHLRALENISRQLRDDTFCRFLKQARTAQDIQQLLAEADSNQFGS"
     gene            complement(1951..2295)
                     /gene="raiA"
                     /locus_tag="VFV87_08000"
     CDS             complement(1951..2295)
                     /gene="raiA"
                     /locus_tag="VFV87_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5103735.1"
                     /translation="MQITISARHGHLSPNTQEKITEKVETVRKFLDRMTAIQVVVDLE
                     HRDSPSVELRVSAEHHDEFVAIDRADTVLAAVDSVIDKMENQLRRFKERIKEHRATGH
                     KHLEPPVPPGEA"
BASE COUNT          450 a          714 c          830 g          526 t
ORIGIN      
        1 ttcgcgagac gccgacgcgc cccagggccg gcatcagcac caggtagcgg ccggggatgc
       61 tgatataggt gctgagcgtc gggcccttgg tgccgatccc ctctttgatc acctggacga
      121 ggacttcgtc gccgcggcga aagacttcct ggattggcgg cttgatgcgc ggccggccgc
      181 cgcgattggt gcggcgatag cgcccgccat tgccacgatc attgccgcgg ccgcgatcgt
      241 tgccgcggcc acgatcgttg ccgcggtcat ggccgcgttc gtcgccgcgg tcgcctgcgc
      301 gcggcggcgg atcgtcgtcg cccacgtcga tatcggccag gcgcccgccc cccatcggct
      361 cgctggggtc gaagccgccc tggcggaaat attgcggctc gacgtcgctg atgtgcaaaa
      421 agccgttgcg gccgacgccg aaatcgacga acgccgcctg gatgctgggc tccaggttga
      481 cgatgcgtcc tttgtagatg ttgccgacga aactttcctg actggctcgt tcgatataca
      541 gctcttcgag aacgccatct tcaatgatgg cgatccggca ttcctccggc tgcgagacat
      601 tgacgagcat ttcgcttttc atgtggacag aatctttcct gttcggattt gcgtggccgg
      661 gagagccgtg ccgcgacgag cgcagcgctt ccgcgagcgg cggcactgct ggccaattcc
      721 aggcgagcaa tggcgcgggg ccgatccgaa atgcggatcg atgcgggcgc agcgggaggc
      781 ggaatgagcg cgcacgagct aatggctgtg gtcgctcggc tatcccgacc tgagagggaa
      841 aaaacgactt gattcaacag tccggctgag agccagccgg tggcgttccc gcagagtggc
      901 gaagccagat caattggctg attggcgatg ccttggagat tcagggagaa agtgggacgc
      961 aatatgggca ttctgcagaa tttagctggt tctattagtt tggaagagaa tgattcgatg
     1021 acttaaggga cgggcgccgt gggccccatc tcgccgaagc cgccttcgac gagctgggag
     1081 agggcgttga cagcgttttc ggcatcgggg ccgatggcgc gtaaagtgag agtcgtaccg
     1141 tttccggccc ccatcgtcaa caagtccagg atgtttttgc cgttgatgac ttcgttgcct
     1201 tttagaatct cgatttggga ctggaactgg ctggcaagct tgacgaacaa atgcgcaggg
     1261 cgagcatgga acccctgcgg gttttggaga gtgaccgctt tgtgggcggt gggttccatg
     1321 cgatttgcct ggggaattgt cggatagccg cgttcgccag aacgcgggcg gggcgctctc
     1381 ccgcactctg gcgagtgcgg ctacgagcta agagccgaat tgattgctgt cggcttcggc
     1441 cagcagttgc tggatgtcct gagccgtgcg ggcctgcttc aggaagcggc aaaaggtgtc
     1501 gtcgcgaagc tggcgggaga tgttctccaa ggcccgcaga tgatcgcccg ggcgatccgg
     1561 aggcgagacc agcagaaaga agaggttcac cttgtcgccg tcgaggctgt tgaaatcaac
     1621 tccttccacg ctcacgccga cggttccgac gagtttttcg acgctggcgt gtttggtatg
     1681 cggaacggcg acgccgcggc caatgccggt gctgccgagt tcttcgcgct tcatgatggc
     1741 cgcaacgatg ccttcaacgt ccgcaggttt aacggcgccg gcggcggcga gtgattcgac
     1801 cagttcgcgg atgatggccg gcttttcatc ggtggccaga tcggcgcgaa tggccgcaac
     1861 gctgataaaa tcgctgaatc tcatggaagg ttgtcctcct ggtgagcctg aagcgacacg
     1921 tttactgccg ccgcccacca cttgccgcaa ttatgcttcg cccggcggca cggggggttc
     1981 aaggtgctta tgaccagtcg cccggtgttc tttaattcgt tccttgaagc ggcgcaactg
     2041 gttttccatt ttgtcaatga cgctatcgac cgcggcaagt acggtatcgg cccgatcgat
     2101 ggcgacgaac tcgtcgtggt gctcggccga aacgcgcagc tcgacgctcg ggctgtcgcg
     2161 gtgctccaga tcgacaacga cctgaatcgc agtcatgcga tctaagaatt tccttaccgt
     2221 ctccaccttt tcggtgattt tttcctgcgt gttggggctc aggtggccgt gccgcgccga
     2281 aatggtgatc tgcacgctgc aaactccaaa gaaggatcgg ctggatgtcc gtgaggggcg
     2341 gtttcccggc ctggaaactt cggattgtag cgtcctcgcg atgggacggt aatccccgta
     2401 cgtgagccgc gaatggggac gaagcgagtc ccgccttcta gtttagctga tggcaggcgc
     2461 ggggaaaggg ccggggggaa cgcacaagat cggcttgacc gcagcccctc cctattccaa
//
LOCUS       DASUGB010000415         5380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_741122, whole genome shotgun sequence.
ACCESSION   DASUGB010000415 DASUGB010000000
VERSION     DASUGB010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741122"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="VFV87_08005"
     CDS             <1..2355
                     /locus_tag="VFV87_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX5103736.1"
                     /translation="GAVTHANPMRQHVNRGEALFHSIGCVACHDPRKAPPQPPLPTSI
                     PLGTPSRKYTLPGLTKFLEDPLAIRPSGRMPHLSLSPADARDIASYLLNDLDITSGLQ
                     YAYYEGSWDKLPDFSKLEPKEVGDASAFDVSVAKRKDHFGLRFDGTILLDKDGEYRFL
                     IGSDDGSRLLIDDKEIVKVDGIHPFQQARKALQMKAGLHTVAVEYFEQGGEETLQVDF
                     EAPGKTQMPLAAIITAPATQKGSAPPPEDFVVDPSKAAKGREYFASLGCASCHELKVG
                     AEKVASTRSAPSLASLKGQGGCLESVVGILPAKNPRYTLSPRQRETLAAAIAAAKLPP
                     KDLPPAELVNRSLIRFNCIACHSRGELGGVEDARNPHFQSDMPEMGDEGRLPPHLTGV
                     GAKLNPNWLKTVFEQAPKDRPYMFTRMPKFGLQNVGQLVPALEQADESAIVPISKLDI
                     DPADKKFKAAGRRLVGAQGFSCIKCHTFADKRSTGIQALSLTTMTKRLRPEWFHQYMQ
                     SPLTYRPGTRMPTPFPDGKTTLPDILDGSVEKQTASIYAYLSDADQAQLPVGLVTGQM
                     EILAIDEAVIYRNFIEGAGTRAIGVGYPEKLNLAFDANDLRLAMLWHGGFIDANRHWN
                     GRGAGFEGPLGDNILRLPEGPPLAVLEGNDSPWPKEIARDSGYQFRGYKLGEKMRPTF
                     LYSFDGVAVEDTPQPIGDQDVYVMHRRLKFSSDKPPAHLTFRALTGGKIDDLGGGNFK
                     IDEKWMLHVDSKLKPILRQQGDTELLIPITFENNQAEIQLTYDW"
     gene            2456..3934
                     /locus_tag="VFV87_08010"
     CDS             2456..3934
                     /locus_tag="VFV87_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103737.1"
                     /translation="MRSKLVLIVGILLVAAPVFAQPDKPHTEDDYYRLVRFPMTEQVV
                     LEAGALEMLPDGKLAVATRRGDIYLVEQPFAAEPEQIFPLPFARGLHEVLGLAWKDGW
                     LYCVQRCDVTRIKDSDGDGRADVFEVVADGWEISGDYHEYAFGSKFDKEGNLWVTLCL
                     TGSFSSEVKYRGWCLRITPDGKVIPTTSGLRSPGGVGANAAGDMFYTDNQGPWNGACA
                     LKWLRPGAFVGHPGGNRWYEVTGGVMGQRPAEPQSGSRMMTEAQKIPQLEPPAVYFPY
                     GKMGQSAAGIACDTTGGKFGPFQNQLFVGDQTHSTVMRVDLEKVKGHYQGACFPFRQG
                     FGSGSLSLLMTKEGSLFVGGTNRGWGSRGSQPYSLDRLDWTGKTPFEIHEMHALPTGF
                     ELTFTEPVEAATAGDPKSYKLSTYTYIYQAAYGSPEVDPTEPKITKIEVSPDGKKVRL
                     TIDKLQEGHVHELHLDGVRSAAGQPLLHPAAYYTLNYIPDLT"
     gene            3931..4557
                     /locus_tag="VFV87_08015"
     CDS             3931..4557
                     /locus_tag="VFV87_08015"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5103738.1"
                     /translation="MTTATTQIENPKAAMRRAAYDARNAEPDKDRASELAVARLVALP
                     EYHAAQTVMWYLDCRSELRTRQALPAALESGKRIVVPYCTVDEVGANKLGLWLLESMD
                     ELIVGKWKILEPPRERWGQPGKEIPPTELDLVVVPGVAFSRSGGRMGNGQGYYDRLLA
                     TVRPDCPLVALCYECQLFDDLVVGPHDVFMNKVITERAAYEGRIRRPR"
     gene            4674..>5380
                     /locus_tag="VFV87_08020"
     CDS             4674..>5380
                     /locus_tag="VFV87_08020"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: protein motif:HMM:NF014024.4"
                     /note="catalyzes the transfer of a formyl group from
                     formylmethanofuran to tetrahydromethanopterin
                     tetrahydromethanopterin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HEX5103739.1"
                     /translation="MPAVIEDTYAEAFRSIYVEFLITARDRRWVEQAAAAATGNASST
                     ILCDCEAGVDRYVGPGGDESFATPDGRPGAIVQLHVPRFRKDRLAALERAALIRISQN
                     VLTCPTAACFNLIDSPEYFRLGRKIAYFGDGFQRRIERYGRKLWWIPTLGGEFVLDRR
                     FGYADGLMGGNLWYFGETADAALAAAEAGAAAASDCPGVILPFPGGVAGSGSKAGSKY
                     KFSIASTYEKYCPLLAG"
BASE COUNT         1005 a         1826 c         1630 g          919 t
ORIGIN      
        1 ggcgccgtca cacacgccaa cccgatgcgg cagcacgtca atcgcggcga ggcgctgttt
       61 cactcgatcg gctgtgtggc ctgccacgac ccgcgcaaag ccccccctca gccgccactg
      121 ccgacgtcga ttccgctggg cacgccgtcg cggaaataca cgctgcccgg gctgacgaag
      181 ttcctcgaag acccgctggc catccgtccc agcggccgca tgccgcacct gagtctctcg
      241 ccggctgacg cccgcgatat cgcctcgtat ctgctgaacg acttggatat cacgtccggc
      301 ctgcagtacg cgtattacga gggctcctgg gacaaactgc ccgatttcag caagctggag
      361 ccgaaggagg tgggcgacgc atcggccttc gatgtcagcg tggccaaacg caaagaccac
      421 ttcggcctgc gattcgacgg cacgatcctg ctcgataagg acggcgagta tcgcttcctg
      481 attggctccg atgacggttc gcggctgctc atcgacgaca aggagatcgt caaggtcgat
      541 ggcattcatc cgttccagca ggcccgcaag gcgctgcaga tgaaggccgg actgcacacg
      601 gtcgccgtcg agtatttcga gcaagggggc gaggagacgc tgcaggttga tttcgaggcc
      661 ccgggcaaga cccagatgcc gctggcggcg atcatcaccg cgcccgccac gcaaaagggt
      721 tccgctccgc cgccggaaga cttcgtcgtc gatccctcca aagctgcgaa gggacgagag
      781 tattttgctt cgcttggctg tgcgtcgtgc cacgaactga aagtcggtgc ggagaaggtt
      841 gcctcaacca ggtcagctcc ctcgctggca tcgctcaaag gccaaggcgg ctgcctggaa
      901 agtgtggtag gcatcctgcc tgccaagaat ccgcgttaca ccttgagccc ccgccagcgc
      961 gaaacgctcg ccgctgccat cgctgccgca aaattgcctc ccaaagacct accccccgct
     1021 gagctcgtca accgctcgct cattcgcttc aattgcatcg cctgccacag ccgcggcgag
     1081 ctaggcggcg tcgaagacgc ccgcaacccg catttccaat ccgacatgcc ggaaatgggc
     1141 gacgaaggcc gcctgccgcc gcacctgaca ggcgtgggag caaagctcaa ccccaactgg
     1201 ctcaagacgg tcttcgagca ggctccgaaa gaccggccct acatgttcac tcggatgccg
     1261 aagttcggct tgcaaaacgt cggccaactt gtccccgctc ttgagcaggc cgacgaatcg
     1321 gcgatcgtgc caatatcgaa gctcgatatc gaccccgccg acaaaaagtt caaggccgcc
     1381 ggccgccgac tcgtcggcgc gcagggcttt tcgtgcatca agtgccacac ctttgcggac
     1441 aagcgctcga ccggcattca ggccctcagc ctgaccacga tgaccaagcg cctgcggccg
     1501 gagtggtttc accagtacat gcagagcccg cttacctatc ggcccggcac gcgaatgccc
     1561 acgccattcc cggatggcaa gacgacgctg ccggacattc tcgacggcag cgtggagaag
     1621 cagacggcct cgatctacgc ctatctgtcc gatgccgacc aggcccagct tcccgtgggc
     1681 cttgtcaccg ggcaaatgga aatcctcgcc atcgacgaag cggtcattta tcgcaatttc
     1741 atcgaaggcg ccggcacgcg ggccatcggc gtcggttacc cggagaagct gaacctggcg
     1801 ttcgatgcca acgacctgcg gttggcgatg ctttggcatg gcggctttat cgacgccaac
     1861 cggcactgga acggccgcgg ggcgggcttc gaaggcccgc tgggcgacaa catcctccgc
     1921 ctgccggaag gcccgccgct ggcggtgctc gaaggcaatg attcgccctg gcccaaggaa
     1981 attgcccgcg acagcggcta tcagttccgc ggctacaagc tgggcgaaaa aatgcggccg
     2041 acgttcctct attcctttga tggagtggcg gtcgaagaca cgccgcagcc gatcggtgac
     2101 caggacgtct acgtcatgca ccgcaggctg aagttttcgt cggataagcc accggcccat
     2161 ctgacctttc gcgccctgac cggcggcaag atcgacgacc tgggcggcgg aaacttcaag
     2221 atcgacgaga aatggatgtt gcacgtcgat tccaagctca agccgattct ccgccagcaa
     2281 ggcgacacgg aactgctgat tcccattacg ttcgaaaaca atcaagccga aatccaactc
     2341 acctacgact ggtaggggca agccgcgctc ggccatcggc gagcggcgag cggggggcgt
     2401 cagccccctg aggccaacct cgtcccattc ccaaccaacc cgcagactca ccacgatgcg
     2461 ttccaaactc gtcctgattg tcggaatcct actggtcgcc gccccggtct tcgctcagcc
     2521 cgacaagccc cataccgagg acgactatta ccgccttgtc cgcttcccga tgactgaaca
     2581 ggtcgtcctg gaagcgggcg cgctggagat gctgccggac ggcaagctcg ccgtcgccac
     2641 gcgccggggc gatatctacc tcgtcgagca gccttttgcc gccgagcccg agcagatttt
     2701 cccgctgcct ttcgcccgcg gcttgcacga ggtgctgggt ctggcgtgga aggacggctg
     2761 gctgtactgc gttcagcggt gcgacgtgac ccgcatcaag gactccgatg gcgacggccg
     2821 ggccgacgtt ttcgaggtcg tcgccgatgg ctgggagatc agcggcgact accacgagta
     2881 cgcgtttggc agcaaattcg acaaggaagg caatctctgg gtgacgctct gcctcaccgg
     2941 ctcgttcagc agcgaggtca agtatcgcgg ctggtgcctg cggatcacgc ccgatggcaa
     3001 ggtgattcct accacgagcg gtttgcgctc gcccggcggc gtcggcgcca atgcggcggg
     3061 cgacatgttc tataccgaca atcagggacc gtggaacggc gcctgcgccc tcaagtggct
     3121 gcggcccggc gcgtttgtcg gccatcccgg cggtaatcgc tggtacgaag tcacaggcgg
     3181 cgtcatgggc cagcgccccg ccgagcccca gagcggcagc cgcatgatga ccgaggccca
     3241 gaagattccg caacttgagc cgccggcggt ctatttcccc tacggcaaaa tgggtcaatc
     3301 cgcggccggc atcgcgtgcg acacgaccgg cggcaagttc gggcccttcc agaaccagct
     3361 cttcgtgggc gaccagacgc acagcaccgt catgcgggtc gatctcgaga aggtcaaagg
     3421 ccactatcaa ggcgcgtgct tcccattccg gcagggtttc ggcagcggct cgctttcgct
     3481 cctgatgacc aaagagggct cgctgttcgt cggcggcacc aatcgcggct ggggctcgcg
     3541 cggctcgcag ccgtactcgc ttgaccggct ggattggacg ggcaaaactc cgtttgaaat
     3601 tcacgaaatg cacgccctgc ccaccggctt tgagctgacg ttcaccgagc cggtcgaggc
     3661 ggccaccgcg ggcgatccga agtcctataa gctcagcacc tacacctaca tctatcaggc
     3721 cgcctacggc agccccgaag tcgatccgac ggagccgaaa atcaccaaga tcgaagtttc
     3781 gccggacggc aaaaaggtcc gcctgacgat cgacaagttg caagaggggc acgtccacga
     3841 actgcacctc gatggcgtcc gctccgccgc cggccagccc ttgcttcatc ccgccgcgta
     3901 ttacacgctt aattacatcc cggacctcac gtgacaaccg ccacaactca aatcgaaaac
     3961 cccaaggccg cgatgcgccg ggccgcgtat gacgcgcgga atgccgagcc ggacaaggac
     4021 cgcgccagcg agctcgccgt tgcccgtctc gtcgcccttc ccgagtacca cgccgcccaa
     4081 acggtgatgt ggtatctcga ctgccgtagc gagctccgca cgcgccaggc cttgcccgcg
     4141 gcactggaaa gcggcaagcg gatcgtcgtc ccctactgca cggtcgatga agtgggggcg
     4201 aacaagcttg gcctctggct gctggaaagc atggatgagc tgatcgtcgg caaatggaaa
     4261 atccttgagc cgccgcgcga gcggtggggt cagccgggca aggagattcc gccgaccgag
     4321 ctcgatctgg tcgtcgtccc cggcgtcgcc ttcagccgtt ccggcggccg gatgggcaat
     4381 ggccagggct attacgaccg attgctggcg accgtgcggc ccgattgccc gctcgtggcc
     4441 ctgtgttacg aatgtcagct tttcgacgac ctggtcgtcg gcccgcacga cgtcttcatg
     4501 aacaaggtga tcaccgagcg agccgcgtac gaaggccgca tccgccggcc gcgctaatgt
     4561 cgcactcgcc ggagccgcac tcgccgtagc cgcactcgcc agagtgcggg cgtattccgc
     4621 gctgatgctc gctcgcccgc gttctggcga acgcggctac ttcctactca gccatgcccg
     4681 cggtcatcga agacacttac gccgaagcct tccgcagcat ctatgtcgag tttctgatca
     4741 ccgcccgcga tcgccgctgg gtggagcagg ctgcggcggc tgcgacgggc aacgcctcga
     4801 gcacgatcct ctgcgactgc gaagcgggcg tcgatcgcta tgtcggtccc ggcggcgacg
     4861 agtcgtttgc cacgcccgac ggccggccgg gcgcgatcgt gcaactgcac gtgccgcggt
     4921 tccggaagga tcgcctcgcc gccttggagc gcgccgccct tatccgcatc agccagaacg
     4981 tcctcacctg cccaaccgcg gcctgtttca acctgatcga ttcgcctgag tatttccgcc
     5041 tcggccgcaa gatcgcctat ttcggcgacg gctttcagcg gcggatcgaa aggtacggcc
     5101 gcaaactgtg gtggattccc acactcggcg gcgagttcgt cctcgaccga cgcttcggct
     5161 acgccgacgg cctgatgggg ggcaacctgt ggtatttcgg cgagaccgcc gatgcggccc
     5221 tcgccgccgc cgaagccgga gcggccgccg ccagcgattg tcccggcgtc attcttccct
     5281 tccccggcgg cgtcgccggc agcggctcca aagccggcag caagtacaag ttctcgatcg
     5341 ccagcaccta tgagaaatac tgtccgcttc tggccgggga
//
LOCUS       DASUGB010000416         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_601011, whole genome shotgun sequence.
ACCESSION   DASUGB010000416 DASUGB010000000
VERSION     DASUGB010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601011"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFV87_08025"
     CDS             complement(<1..421)
                     /locus_tag="VFV87_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103740.1"
                     /translation="MRLRLSRLGFLNLSTACLLGMSAVARAQEPIVPPDAKLEKLFEG
                     QYLTEGVTAAPDGTIYFSEITFSHLSRDKNGAIEAGYIWKHDSASGKTTVFRSPSGMS
                     NGLKFDADGNLLAAEGADYGGRRVTRTDMTTGKTYIIA"
     gene            complement(503..2989)
                     /locus_tag="VFV87_08030"
     CDS             complement(503..2989)
                     /locus_tag="VFV87_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5103741.1"
                     /translation="MMQRIAATVLMTLAGGGLQAADPPLAPVSQRFADSNTPETPDFQ
                     RHVVPLLGTLGCNGRACHGSFQGQGGFRLSLFGYDFEADHAALAGGDEPRVDAKAPHD
                     SLALLKPTRQIDHDGGKRLDIDSWQYRLLSKWIAGGAKPVADDAADFAALEIEPAEVL
                     LVKAGESVQLRVIARWSDGSREDVTPLCRYQTRDESIAVIDAGGKITSAGPGDTHVVV
                     FYDNGVVPVPVIRPVTNLTGPQYPAASASTKIDELVLAKLRKLGVVPSERCTDEEFLR
                     RASLDLTGTLPLPEEVRAFAADQSADKRRAKIDELLSRPTYAAWWATKFGDWTGNGEQ
                     TGPVGGEQGLRRRFSELWYEWLDRRLDENVPYDQIAAGIVLATSRRPGQSFDEYCTEM
                     SGYFRGENAADFAARETMPYFWSRRALGPNEEKARAFAYSFLGVRLECAQCHKHPFDQ
                     WTKNDFDQFAAFFNGVRYGAGNRNEYQAMKDGAGLKGLDEDSGDYKRKFVKLLEDGEV
                     MPFKEVTVPPASSRAKRRPPKAAGGKIGRVITPRLLGGEQVLTEEYDDPRQPLMDWLR
                     QEDNPYFARALVNRVWANHFGVGIVEPPDDMNLANPASNAALLDHLTREFVAHAYDLK
                     WLHREILASDTYQRSWQPNETNRLDERNFSRAILRRLPAEAIYDALVQATAGADEQAA
                     LVHDDQRVRSRAIGLASGYSPARDEKLYAVYLFGKPAREVACDCERSSSPSLQQTLFL
                     RNDAELLGLIDRKDGWLASLKSAAKKSAELPASDSLIDEAYLRTLNRLPTADERRTAL
                     AHLAEGEDPLAGLSGLLWALLNTKEFVVNH"
     gene            3209..3787
                     /locus_tag="VFV87_08035"
     CDS             3209..3787
                     /locus_tag="VFV87_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103742.1"
                     /translation="MRSLLCLLTLVAASLNTASATAQSFSFGIGTGGYGHHHHHGGWH
                     FDYHDCWHDHYYYPRYVYAYPAPVIRERVTYVEQTPSVSRVEPPIADTLITVQNAAGK
                     SVAVSFLMDAQDVALRDGEVRTFSGRSSRVVEFDRGGDFGSARYDLAPGDYQFVISDR
                     GWDLVRKPAAAASTATRPSVRKNALPAETTLR"
     gene            complement(3868..4461)
                     /locus_tag="VFV87_08040"
     CDS             complement(3868..4461)
                     /locus_tag="VFV87_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103743.1"
                     /translation="MASPQTSPSFLRISLLELLLLLAAVAVGCAALKYAGEVWWIVLS
                     SALLVGFLAAAVVAVVGRGPGQARAIGFVLCVMIYMPLIWAAPLDTSRELDPHAGRLP
                     TTKLLGAIFESIVSRKQVDMATGQPVANSNSGYSGAYPPTLGYSMTLTQESPDRGQFM
                     AIGHLLWALLLGYLGSRLAVWVDGRRRQRAEPRLVAS"
     gene            4592..>4792
                     /locus_tag="VFV87_08045"
     CDS             4592..>4792
                     /locus_tag="VFV87_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103744.1"
                     /translation="MARPSQFLLDFRKTIEFVLSQVGGLSLRDFIKLYDFDEFLRGEA
                     AECESPRKLAIAFALRFLAEVKP"
BASE COUNT          830 a         1598 c         1499 g          865 t
ORIGIN      
        1 cggcgatgat ataggtcttg ccggtggtca tgtcggttcg cgtgacgcgc cggccgccgt
       61 agtccgcccc ctcggccgcg aggagattgc catcggcgtc gaacttgagg ccgttcgaca
      121 tgccgctcgg cgagcggaag acggtcgtct tgccgctggc ggagtcgtgc ttccagatgt
      181 atcccgcctc gatcgccccg ttcttgtcgc gcgagaggtg cgaaaacgtg atctcgctga
      241 agtagatcgt gccgtccggc gccgcggtca cgccttcggt caggtactgc ccttcgaaga
      301 gcttttcgag cttcgcatcc ggcggaacga tcggctcctg agcgcgggcc acggcggaca
      361 tgccgagcag gcaggcggtc gacagattca gaaagcccag acgggagagg cggagtctca
      421 tggtgcatcc ttcggcgagg tggggtgact ttacacggca acccgagcag gcagccagga
      481 taagctctgc cgatcggccg aatcaatgat tgaccacgaa ttccttggtg ttgagcaggg
      541 cccacagcag gcctgaaagc ccagcgagcg ggtcctcgcc ttcggccaga tgggccagcg
      601 ccgtgcggcg ctcgtctgca gtgggcagcc gattgagcgt gcggaggtag gcttcgtcga
      661 tcagggagtc agaagcaggc agctcggctg atttcttcgc ggcggacttc aggctcgcca
      721 gccagccgtc cttgcggtca atcaggccca gcagctcggc gtcgttccgc agaaagagcg
      781 tctgctgcag gctggggctg ctcgatcgtt cgcagtcgca ggcgacttcg cgggccggct
      841 tgccgaacag atagacggcg tagagcttct cgtcccgcgc gggactatag ccgctggcca
      901 ggccgatcgc ccggctgcga acgcgctggt catcgtgaac cagcgccgct tgctcgtccg
      961 cgccggcggt cgcctgcacc agcgcgtcgt agatcgcttc cgccggcagc cgccgcaaaa
     1021 tcgcccggct gaaattccgc tcgtccaggc ggttggtctc gttcggctgc caggagcgct
     1081 gataggtgtc gcttgccagg atttcgcggt gcagccattt gaggtcgtag gcgtgggcga
     1141 cgaattcgcg agtgaggtgg tcgagcaggg ccgcgttgct cgccggattc gccaggttca
     1201 tgtcgtccgg aggctcgacg atgccgacgc caaagtgatt ggcccacacg cggttgacca
     1261 gggcccgcgc gaaatacgga ttgtcctcct gccgcagcca atccatcagc ggctggcggg
     1321 ggtcgtcgta ctcttcggtc agcacttgct cgccgcccag cagccgcgga gtgatgactc
     1381 ggccgatctt gccgccggcg gctttcggcg gccggcgctt ggcccgcgac gaagccggcg
     1441 ggaccgtgac ctccttgaag ggcatgactt cgccgtcttc cagcagcttg acgaacttgc
     1501 gtttgtagtc gcccgaatct tcgtcgagcc ccttcagtcc cgcgccgtcc ttcattgcct
     1561 gatattcgtt gcgattgccc gctccgtagc gcacaccatt gaaaaacgcc gcgaactggt
     1621 cgaaatcgtt cttcgtccac tggtcgaacg ggtgcttgtg gcactgggcg cactccagcc
     1681 gcacgccgag gaacgaatag gcaaacgcgc gggccttttc ttcattggga cccagcgcac
     1741 gccggctcca gaaataaggc atcgtctcgc gagcggcgaa atcagccgca ttctcgccgc
     1801 ggaagtagcc gctcatctcg gtgcagtact cgtcaaacga ttgccccggc cggcgactag
     1861 tggccaggac gatgccggcg gcaatctggt cgtaaggcac gttctcatcg aggcggcgat
     1921 cgagccactc gtaccacagc tcgctgaacc tgcgccgcag cccctgctcg ccgccgaccg
     1981 gcccggtctg ctcgccgttg ccggtccagt cgccaaactt tgtggcccac caggcggcat
     2041 acgtcggccg cgaaagcagc tcgtcgatct tcgctcgccg cttgtcggcc gattggtcgg
     2101 cggcgaacgc ccgcacttcc tccggcagcg gcagtgtacc ggtcaggtca aggctcgcgc
     2161 ggcggagaaa ttcctcgtct gtgcagcgct cggaaggaac gacgcccagc ttgcgcagct
     2221 tggcgaggac cagttcgtcg atcttcgtcg aagcgctcgc cgctggatat tgcgggccgg
     2281 tcaggttcgt cactggccga atcaccggca ccggcacgac gccgttgtcg tagaagacga
     2341 caacgtgcgt atcgcccggt ccggcgctgg tgattttgcc gcccgcatca atcacagcga
     2401 tcgattcatc acgcgtttga tagcggcaca gcggcgtgac atcctcgcgg ctgccgtcgg
     2461 accagcgggc gatgacgcgg agctgcacgc tttcgcctgc tttgacaagc agcacctcgg
     2521 ccggctcgat ttcgagcgcc gcaaaatcgg cggcatcgtc cgcgacgggc ttcgcgccgc
     2581 ccgcaatcca cttggagagc agccggtatt gccagctatc aatgtccaac cgcttgccgc
     2641 cgtcgtgatc gatttgcctg gtcggcttga ggagggcgag gctatcgtgc ggtgctttcg
     2701 catccacgcg cggctcgtct ccgcccgcaa gagccgcgtg atcggcctcg aaatcgtatc
     2761 caaacagcga caggcgaaac ccgccttgcc cctgaaaaga cccgtggcag gcccggccgt
     2821 tgcagccgag tgttcccagc agcgggacga catgccgctg gaaatcgggc gtttcgggcg
     2881 tgttgctgtc ggcaaatcgc tggctgaccg gtgccagcgg cggatcggca gcttgaaggc
     2941 cgccgcctgc cagcgtcata agtaccgttg ctgcaatgcg ttgcatcatt ccgcctcggc
     3001 cgccggccaa tccgtgtagg gtgggctgtg cccaccacag acccggtggg cacagcccac
     3061 cctaccactg tgcccaccac agaagtcccg gtgggcgcag cccaccctac cacagatgct
     3121 cggcagccgc ccgctattgt ggcaaaacgc cgcctgccga taaaaggaaa gccaacaagt
     3181 ccacattcgc tcgcggcaag gatgtcccat gcgatcgttg ctctgtctcc tgactctcgt
     3241 cgccgccagc ctgaataccg cctccgccac tgcccaaagc ttctccttcg gcatcggcac
     3301 agggggctac ggccatcacc accatcacgg cggttggcat ttcgactatc acgattgctg
     3361 gcacgatcac tattactatc cgcgctatgt ctatgcctat ccagcgccgg tcattcgcga
     3421 gcgcgtgacg tacgttgagc agacgccgag cgtcagccgc gtcgaaccgc cgatcgccga
     3481 cacgctgatc acggtgcaaa acgcggcggg caagagcgtg gccgtgtcgt tcctgatgga
     3541 tgcccaggac gtggctctcc gcgacggcga agtccgcacg ttctccggcc gcagcagccg
     3601 tgtcgtcgaa ttcgaccgcg gcggcgattt cggctccgcc cgctatgacc tggcgcccgg
     3661 cgactatcag ttcgtcatca gcgaccgcgg ctgggacctg gttcgcaagc ccgccgctgc
     3721 cgccagcacg gccacgcggc ccagcgtccg caagaacgcc ctcccagccg agacgacgct
     3781 ccgttaattg ctcctcgccg ggaccaaact cggtcccgga gcgcccgcgc gcctcaacgc
     3841 cgtagggtgg gctgtgccca ccactgctca acttgcaaca agtcgaggtt ccgcccgctg
     3901 acgccggcgg ccatcgaccc agaccgccag ccggctgccg agatagccga gcaacagcgc
     3961 ccagagcagg tggccgatgg ccatgaactg gccgcggtcg ggggattctt gggtaagcgt
     4021 catcgaatac cccagcgtgg gcgggtacgc tcccgagtaa cctgagttcg aattggccac
     4081 cggctgtccg gtcgccatat cgacttgctt tctgctgacg atcgattcga agatcgcccc
     4141 aagcagcttc gtcgtcggca agcggccggc atggggatcg agttctcggc tggtatccaa
     4201 cggcgcagcc cagatcaatg gcatgtagat cattacgcac aacacgaagc cgatcgcccg
     4261 ggcctgcccc ggcccgcggc cgacgaccgc gacgaccgcc gccgccagaa agcccacgag
     4321 cagcgccgac gagagcacga tccaccacac ctcgcccgca tacttcaaag ccgcacaccc
     4381 caccgccacc gcggccagca agagcagtaa ctccagaagc gagatgcgga ggaagctcgg
     4441 cgaggtttgc ggagaggcca tgagtcggat tgtacctgct tggccaatcg caggtcgaat
     4501 ttcagtggtg ggagcttgac tgtatcccgt tccattggcg agtatcttga cgcaccgtcg
     4561 attcaagggc cagcgtatca agaggacgta catggctcgt ccctcacagt tcctgctcga
     4621 cttccggaag acaatcgaat tcgtcctgag ccaggttggc ggcctttcgc tgcgcgattt
     4681 catcaagctg tatgactttg acgagttcct gcgcggcgaa gcggcggagt gtgagtcgcc
     4741 ccgcaagctg gccattgcct tcgcgctgcg ctttctcgca gaagtgaaac cc
//
LOCUS       DASUGB010000417         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1714320, whole genome shotgun sequence.
ACCESSION   DASUGB010000417 DASUGB010000000
VERSION     DASUGB010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714320"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..814
                     /locus_tag="VFV87_08050"
     CDS             8..814
                     /locus_tag="VFV87_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5103745.1"
                     /translation="MSLAMQAMLLRFSETGEVQTVGLDSVVGRADVRLITATNRDLRV
                     QIAAGEFREDLYYRLNVIHIRIPPLRERGRDVLLLFRHFLRKASEAHRLPPPDLTTEA
                     EELVLAHQWPGNVRELRNVTERIAIRDSNRPITPDDLPAEMRRSDPTAVAVSASGLPG
                     ASEQLQPQGISQHLWDRLMTGADFWEVVQKPFRAHEVTRAELAALIDRGLQETRGSYR
                     ALVRLFNLPPSDYKRFHAFLYQQNCNLPVGPYRTGTASRIKNANGYPVAS"
     gene            complement(845..>2405)
                     /locus_tag="VFV87_08055"
     CDS             complement(845..>2405)
                     /locus_tag="VFV87_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103746.1"
                     /translation="MATGRAPQAERYFVAFADTLGTPAGRFALANYYLSLNRPEDARR
                     VLAETAKRPESFAEATTSLASLDLLSGKTEEARKGIETVLTRAPKHVNALVLKAQLLS
                     NERKYDEALAETGKALAIEPSNPAAHLAEGSIYAATNRLAEAIASYEAALKSDAKNPL
                     QVHEANFRLGSIHLQLGSLDRAMTHAQAALQAQPTSANAHLLAARIETSRGQMSEARR
                     RLAALKGAFPNTAAVANLRGQIELASKQPALAAAAFQEALKHDPANVEALTALARIDL
                     AAGRRKDAVDRVESSLRRPSPSVDLLVLAGQVYGGVGDLPKAEKSLLAAVEREPNRFD
                     AYTMLGQLYASQGRLPDAVKQIEAILSRNPKAVGSNTTLGVLLQMSGRLPEAERAYEN
                     TLAIDPRAGVAANNLAWLLVESRRDLDRALGLAQAASAAMPDAAAVSDTLGWVYYRRN
                     LPSLAIPPLLKAVKAEPRNAEFHYHLGAAYAAAGDKVKARNLLTQALELKLGPNEEKH
                     ARELLASLRAS"
BASE COUNT          389 a          769 c          775 g          472 t
ORIGIN      
        1 gggtgagatg agcctcgcca tgcaggcgat gcttctccgg ttctccgaaa ccggcgaagt
       61 ccagacggtt ggtctggata gcgtggtcgg tcgcgccgac gttcgcctga tcactgcaac
      121 caaccgcgac ctgcgcgtgc aaattgcggc aggggagttc cgcgaggatc tctactaccg
      181 tctgaacgtg attcacatca ggattccgcc gctccgggaa cggggccgcg atgtgctgct
      241 cctcttccgc catttcctgc ggaaggcgag tgaggcgcac cggcttcctc ctccggacct
      301 cacgacggag gccgaggagc tggtgcttgc acaccagtgg cccggcaacg ttcgcgagct
      361 gcgcaacgtg accgagcgga ttgccattcg cgattcgaat cggccgatca ctccggatga
      421 tctaccggcg gagatgcgcc gcagcgaccc gacggccgtg gcggtgtcag cttccgggtt
      481 gcccggggcg tccgagcagc ttcagccgca ggggatttca cagcacctgt gggaccggct
      541 gatgacgggc gcggacttct gggaagtcgt gcagaaaccg ttcagagcgc acgaggtgac
      601 tcgtgccgag ctcgccgcgc tcatcgatcg cggtcttcag gaaacgcggg gcagttatcg
      661 ggcgctcgtg cggctgttca acctgccccc gagcgactac aagcgctttc acgcgtttct
      721 ctaccagcag aactgcaatc tgccggtcgg tccataccgg actggaactg cctccaggat
      781 caagaacgcg aacggctacc cggtggcgtc gtaaagaaat ttacgaattt acgaatttac
      841 gaatttacga cgctcgaagc gacgcgagaa gctctcgagc gtgcttctcc tcgttcggtc
      901 ccagcttcag ctccagtgcc tgagtcagta gattccgtgc cttgaccttg tcgccggcgg
      961 ctgcgtacgc ggcgccgaga tggtaatgga actccgcatt acgaggttcc gccttcacgg
     1021 ctttgagcag cggtggaatc gcaagcgacg gcaggttcct gcggtagtac acccatccga
     1081 gcgtgtcgct gacagcggcg gcgtccggca ttgccgcgga tgcggcctgg gcgaggccaa
     1141 gcgcgcgatc gaggtctcgc cggctctcga ccagcagcca ggcaaggttg ttggcggcga
     1201 cgcccgcacg cgggtcgatc gccagcgtgt tttcgtacgc gcgttccgcc tcaggcagcc
     1261 tgccgctcat ttgcaggaga acgccgaggg tcgtattcga tcccacggcc ttggggttgc
     1321 ggctgaggat tgcctcgatt tgcttcacgg catctggcag gcgtccctgc gacgcataca
     1381 gctgccccag catcgtgtac gcgtcgaacc gattcggttc ccgttcaacg gccgcgagca
     1441 atgatttctc cgccttcggc aggtcgccca ctccgccgta cacctggcca gcgagcacga
     1501 gcagatcgac ggagggactg ggccgccgca gggagctttc cacccgatcc accgcgtcct
     1561 tcctgcgacc cgcggcaagg tcgatcctcg caagcgccgt caacgcttcg acattggccg
     1621 ggtcgtgctt cagagcctcc tggaaagccg cggcagccag ggccggttgt ttcgaagcca
     1681 gctcgatctg ccctcgcagg ttcgcgaccg ccgccgtatt ggggaaggcg cccttcaggg
     1741 ccgcgagacg ccggcgcgct tctgacatct ggccgcggct tgtctcgatg cgggcagcga
     1801 gcaggtgtgc gtttgcgctc gtcggctgcg cctgcagcgc cgcctgcgca tgggtcattg
     1861 cccggtccag agatcccagc tgaaggtgaa tgcttcccag tctgaagttg gcctcatgca
     1921 cctgcagcgg attcttggcg tcgctcttga gtgccgcctc atacgacgca atagcctcgg
     1981 caagccggtt cgtcgcggcg tagatgctgc cctccgccag gtgagccgcc gggttgctcg
     2041 gctcgattgc cagggctttg ccggtttccg caagggcctc gtcgtacttt cgctcgttgg
     2101 agagcagttg cgccttgagc acgagggcgt tgacgtgctt gggggctctg gtcaggaccg
     2161 tctcgatacc ttttcgcgcc tcctcagtct tgccgctcag caaatccaga ctcgcgaggc
     2221 tggtcgtcgc ctctgcaaac gattcaggtc gctttgcggt ctcagccagg acgcggcgtg
     2281 cgtcctccgg ccgattgaga ctcaggtaat agtttgcgag ggcgaaccgg ccagccggtg
     2341 ttcccagtgt gtcggcaaag gcgacgaaat aacgttcagc ctgcggcgcg cggccggttg
     2401 ccatg
//
LOCUS       DASUGB010000418         7662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1158074, whole genome shotgun sequence.
ACCESSION   DASUGB010000418 DASUGB010000000
VERSION     DASUGB010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158074"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFV87_08060"
     CDS             complement(<1..1036)
                     /locus_tag="VFV87_08060"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX5103747.1"
                     /translation="MSRVADKPLTFVSLFAGIEGFGAGLEAAGMRCAFQVEIDPQCRS
                     VLARHWPDVPRWNDVREFDGAATTIRPDWLCGGFPCQDLSVAGRRAGLAGERSGLWHE
                     FHRVVAEFRPSGVLIENVPGLLSSNGGRDMGIVLGGLGQLGYWWAYRVLDAQWFGVAQ
                     RRRRVFIVGCLGDRRRAAEILFERESLPWDSPPSRTARERTAASLASGVSTGSGVNRP
                     GRRREDDFNIVNCLDRQSGGADDNSAQANHLVAFSNRGIDSSCFETLRSDSNGALPMV
                     AHALRSEGADASEDGTGRGTPVVPVYCLQHAQIGRKDDAGPQGKGWQENLGFTLDNRS
                     ASDAVAFCFDE"
     gene            complement(1081..2007)
                     /locus_tag="VFV87_08065"
     CDS             complement(1081..2007)
                     /locus_tag="VFV87_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103748.1"
                     /translation="MQTIKAIETRYRGFNFRSRLEARTAVAFDEMGVEFVYEPEGYDI
                     DGCGRYLPDFWLPNPGCFVEVKPKPLWHPQWIYLAGTCKTRWRSDLNLYGNFAVNPAG
                     SVPDKHDDFASVLFERSVEGLQEADAVAAWIDRADCYATFVEIGMARSMPKRVYVGFD
                     AAYLRVAERSGVCWDYSEPCHDHWFAQLCGDEQLVCESPQGMIDALIAAKRLQAMPDE
                     YHKAQCLANATKKDVWIFFDCGLDEISERNVSLVQAVLCKPGRIIDTRAWPGLIFGNQ
                     ASSRGWQSTRLLTHGLRAARSARFEHGQSGST"
     gene            complement(2009..3373)
                     /locus_tag="VFV87_08070"
     CDS             complement(2009..3373)
                     /locus_tag="VFV87_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5103749.1"
                     /translation="MKADAPVQRTWMERQAELSDLATERHLLASIMLYPQAIDKVSQM
                     VSMTDFFDADLGQAFAVTVDLHRAGRPVSDPALVYSAWKASAVSSSVANIKYVAALIN
                     EVPHGGHAIYYAENVASLSMRRKLLNLADEIGRSVLDRQCKAEELPLWLDGKVKQLPG
                     TATSKARPIADVWTDAIGELQGRVSNPEPSVLLSGFPAADARGFVFGNGELAVLAARP
                     GVGKTSMATQIAMHHAGKGRAVLFASLEMRDKELALRPLVAAAGHNHQLLRTNRIDQE
                     LVNDLRSAKETLGDIPLHVWSPGRVKASAIHAVANLLKASSDLRLLVVDYIGLVRPDD
                     NGRQRYEQVGQIVKSLRDIGQQLQIPVLALCQLNREADSQEPRLANLRESGDIEQDAD
                     VVAFLNPCDAKDETQVDLIVAKDRHGAIGRARLIWHKEQTVFEDPTIPKRHDDFDKFN
                     REGF"
     gene            complement(3315..4157)
                     /locus_tag="VFV87_08075"
     CDS             complement(3315..4157)
                     /locus_tag="VFV87_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103750.1"
                     /translation="MAGDWIKVEKATPRKPEVLRMADILSIHPDHAFGLCVRFWFWCD
                     DNMTDGHAPSVTYVTLDSVFGHAGFTEALLLVGWLVARNGSLQVPNFDRHLSESAKNR
                     ALSGERKRNQRSRAERDKNVTREEKRRDRKKVPKKEPTTIPELLQTPEFSAAWKSWEQ
                     HRSEKKSPLTPTTVVAQLKMLARMGPERAVAAIEHTIFKGWTGLKEPDADDKPPPPRP
                     PPVNAPPPPKRVSPVNFKNDPYATKTAPSPPASPPVPPPPGASGEGRRAGAANVDGAT
                     GGAV"
     gene            complement(4265..4834)
                     /locus_tag="VFV87_08080"
     CDS             complement(4265..4834)
                     /locus_tag="VFV87_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103751.1"
                     /translation="MQPVDRKGIFRAEIVEYGLKQVDSGSVAVSLRCKLLEMWDGQEW
                     QPWAQYEMEARGDVWVVKKDGNTNDGAAESLMKHAGWDASFSSIVNETWQPTACQVTI
                     EEETYKNQVQRKIGFINAWDRTPGAMSNVDGAKAADLENRFGASLRALRGNLSRNVPP
                     VATNGNGRPSAPPAPPAEPAMAGERPIPF"
     gene            complement(4846..5691)
                     /locus_tag="VFV87_08085"
     CDS             complement(4846..5691)
                     /locus_tag="VFV87_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5103752.1"
                     /translation="MSTAAPPKPPPPAPPPPAGPNSQSRAIGSAPARKLSVTTGRSIG
                     AQRVGIYGTGGIGKTKLASLLKEVGINPLIVDVENGSRFLNISRVGDIQTWDELRGVL
                     HDESLLAPYGAVVIDSFTKAEELAIAHTLATVPTEGGEFVDKIEGYGYGKGYTHAYET
                     FLKILGDLDAVIRRGKHVVVICHDFKASVPNPYGEDWIRFEHRMQETPKGPIRSRVFE
                     WCDHFFYVGYDVSVTKDGKGKGAGTRTIYPSEMPTHKAKSRDVSSTVIYRDGEPDLWK
                     LLFSK"
     gene            complement(5606..6736)
                     /locus_tag="VFV87_08090"
     CDS             complement(5606..6736)
                     /locus_tag="VFV87_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5103753.1"
                     /translation="MKQLLTHSRQDAFKTCRKRHYFAYELGLRRIDDAKALRMGSAFH
                     EGIESLGKGLGLVAACQAVRSKYTRCPVFMNPAEWSYERETVLRLVCAYDWRWASHPL
                     ENVAVELPFELPLVNPSTGKRTTLFNLAGKIDGIVKMEDGRLAVKESKLLGDDIGHDS
                     DLWRRLRIDHQISLYMVAARQLGYPVQTVLYDVTRKPTIAATDVPVLDELGAKIVLNE
                     FGTRVKTERGQWRQTGDKDKGYVLQSRPMTTEEWGEKLSKDIAARPDYYFARVEVPRL
                     DQDLDEYQAELWEIQQLIRDAQKNNRWFRTVNKNTCGFCPYFGICSGGSSFNGTPPEG
                     FELVSDCHPELERTHVNRSTAETTAPSTATASRPQFAEQSYW"
     gene            complement(6733..6936)
                     /locus_tag="VFV87_08095"
     CDS             complement(6733..6936)
                     /locus_tag="VFV87_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103754.1"
                     /translation="MSGPKLYKWVYPLALRQLPDGWWLDAAMVTPDVPLWGPYHTKRE
                     AAEARDSYLKGHPEVKKRKVECQ"
     gene            complement(6933..7124)
                     /locus_tag="VFV87_08100"
     CDS             complement(6933..7124)
                     /locus_tag="VFV87_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103755.1"
                     /translation="MTTATTAIVADMERSRKEGLELIDMTRDQASKGAPDGIDTAKLL
                     AGERREVRKYRRIKKGRTA"
BASE COUNT         1434 a         2406 c         2155 g         1667 t
ORIGIN      
        1 gttcgtcgaa acaaaaggcc acggcatccg acgcggaacg attgtcgagc gtgaatccaa
       61 gattctcttg ccagcctttg ccttgcggtc ccgcgtcgtc ctttcttccg atctgtgcgt
      121 gttgcaagca atacacaggc actactggcg tccctctccc ggttccatct tcgctagcgt
      181 ccgcgccttc ggagcgcagg gcatgggcaa ccataggcaa cgcaccgttg ctgtccgacc
      241 tcaatgtttc aaaacaagat gagtcaatgc cgcggttaga aaacgccacc aaatgattcg
      301 cctgtgctga gttatcatcc gcaccaccac tttgccgatc caggcaattt acaatgttga
      361 aatcgtcctc tctgcgtcgt cctggccggt tgactccgct acctgtgcta acgccgctcg
      421 caaggcttgc ggcagttctt tcccgcgccg ttcggctcgg cggagaatcc cacggcaggc
      481 tttcgcgctc aaaaagtatt tctgcggcac gtcgcctgtc tccaagacat ccgacaatga
      541 acacacggcg gcggcgttgg gccactccga accactgagc gtcaagcacg cggtaggccc
      601 accaataccc caattgcccc agccctccga ggacgattcc catatcccgt ccgccgttgc
      661 tcgacaaaag gccggggacg ttttcgataa gaaccccgct gggacggaac tcggcaacga
      721 cgcgatggaa ttcatgccag agtccgcttc gctcgccagc caatccagca cgccgtccag
      781 ccaccgaaag gtcttggcag gggaatcctc cgcaaagcca gtctggtcga atagttgttg
      841 ctgctccatc gaactccctc acgtcgttcc atcgcgggac atccggccag tggcgggcca
      901 gcacgctgcg gcattgcggg tcaatctcta cttggaaagc acatcgcatc ccggctgctt
      961 ccaagccagc gccgaagccc tcgatgccgg cgaacagcga gacgaacgtc agtggtttgt
     1021 cggctacgcg gctcatgggg tggtctccag cagggcgcgg catgggtgtg tggtcattgg
     1081 ctaggtggat cccgattggc cgtgctcgaa gcgagcggag cgagcagcgc gaagtccgtg
     1141 cgttagcaat ctcgtcgatt gccagccgcg actagatgct tggtttccaa agatcaatcc
     1201 cggccaagcg cgcgtgtcga tgatgcggcc tggcttgcac aggaccgctt gcaccaagga
     1261 cacatttctc tcgctgattt cgtcgagtcc gcagtcgaag aaaatccaga catccttctt
     1321 tgttgcgttc gccaagcatt gtgccttgtg gtattcgtcg ggcattgctt gcagtcgctt
     1381 agccgcaatc agcgcatcga tcatgccttg cggagattcg caaacaagtt gctcgtcgcc
     1441 acacaactga gcaaaccaat gatcgtgaca tggttcgctg taatcccagc acacgccact
     1501 acgttcagcc actcggagat acgccgcgtc aaatcccaca tagactctct tgggcattga
     1561 tcgagccatg ccgatttcta caaacgtcgc gtagcaatcc gcgcggtcga tccatgcggc
     1621 aacagcatcc gcctcctgca aaccctcaac ggacctctca aatagcacgc ttgcaaaatc
     1681 atcgtgcttg tccggtacgc tccctgcggg attgacagca aagtttccgt agaggtttaa
     1741 gtccgaacgc caccgagtct tgcatgttcc ggccaggtaa atccactgcg gatgccaaag
     1801 tggcttcggc ttcacctcaa caaaacagcc tggattcgga agccaaaaat ccggcaggta
     1861 tctaccacag ccgtcaatgt catagccctc cggctcatag acgaattcga ctcccatctc
     1921 gtcgaaagcc acggctgtcc gggcctctag cctgctccga aaattaaatc cacgatagcg
     1981 cgtctcgatc gccttgattg tttgcatctc agaacccctc ccgattgaac ttgtcgaagt
     2041 catcgtgccg cttcggtatc gttggatctt caaacaccgt ctgctctttg tgccaaatga
     2101 gtctcgcccg gccgattgcg ccgtggcggt ccttagccac aatcaggtcc acctgcgtct
     2161 cgtctttggc gtcgcacgga ttcaggaacg ccaccacgtc ggcgtcttgc tcgatgtctc
     2221 cagactcgcg caagttcgct aggcgtggct cctgggaatc ggcttcgcga ttgagctggc
     2281 acaaggcgag aactggaatc tgcaattgct gcccgatgtc gcggagtgat ttgacaattt
     2341 gcccaacttg ctcgtatcgc tgccggccgt tgtcgtcggg gcgaacgagc ccgatatagt
     2401 ccactacgag cagccgcaaa tcgctgctgg ccttgagaag attggcaacg gcatggattg
     2461 ccgatgcctt gacgcggcct ggagaccaga cgtgcagtgg aatgtcgcca agcgtttcct
     2521 tagcagaccg caagtcgttc acaagctctt ggtcaattct gtttgtgcga agcagctggt
     2581 ggttatggcc ggcggctgcc acgagcggtc gcagggcgag ttccttgtct cgcatttcca
     2641 acgaggcaaa cagaacggcg cgccccttcc ccgcgtggtg catcgcaatt tgggttgcca
     2701 tcgaggtctt gccgacaccg ggacgtgcgg caagaaccgc caactctcca ttgccgaaca
     2761 cgaagccgcg ggcgtctgcg gcagggaagc cagacagaag caccgacggc tctggattac
     2821 tcacccggcc ctgcaactcg ccgattgcat ccgtccacac atcggcgata gggcgggcct
     2881 tggaggtggc tgttcctggc agttgcttaa ctttgccgtc aagccagagc ggcagctctt
     2941 ccgccttgca ctgccggtcg agaactgagc gccctatttc gtccgcgaga ttcaggagct
     3001 ttcgccgcat cgacagcgat gcgacattct cggcgtagta aatcgcgtgc ccaccgtgcg
     3061 gtacttcgtt gatgagcgct gccacatact tgatgttggc gacggacgat gacactgccg
     3121 acgccttcca cgccgaatac acgagcgccg ggtcgctgac tgggcggcct gcgcgatgca
     3181 agtccacggt gacagcgaat gcctgcccca ggtccgcgtc gaaaaaatcg gtcatggaaa
     3241 ccatttgcga tactttgtcg atggcttgcg ggtaaagcat gatgctggct aggaggtgac
     3301 gttcggtcgc gaggtcagac agctccgcct gtcgctccat ccacgttcgc tgcaccggcg
     3361 cgtcggcctt caccgctggc tcctggtggc ggtggaaccg gcggactggc ggggggactg
     3421 ggggcggttt tggtggcgta ggggtcattc ttgaaattca ctggcgagac tcgcttcgga
     3481 ggcggtggtg cgtttactgg cggcggcctg ggggggggcg gcttgtcgtc ggcgtccggc
     3541 tccttgagcc cagtccagcc cttgaaaata gtgtgttcga ttgcggcgac tgctcgctcc
     3601 ggccccatgc gcgcaagcat cttgagttgg gcaacaacag tcgttggcgt cagcggggat
     3661 ttcttctcgc tgcgatgctg ctcccaagat ttccacgccg cgctgaactc tggcgtttgc
     3721 agaagctcgg gaattgtcgt tggctctttc tttggtactt tctttctatc tcttctcttc
     3781 tcttctctgg tcacgttttt gtcacgctcg gcgcgtgacc gctgatttct ctttctctcg
     3841 ccggaaagtg ccctgttttt cgcactttcg gacaagtgcc gatcaaagtt ggggacttgt
     3901 agcgagccgt tgcgggccac aagccagccg actaagagga gcgcttcggt gaaaccagcg
     3961 tgaccaaaaa cggaatccag cgtgacatat gtcacgctcg gcgcgtgacc atctgtcatg
     4021 ttgtcgtcgc accaaaacca gaaccgaaca caaagaccaa atgcgtgatc tgggtggatc
     4081 gacaaaatgt ccgccattcg caaaacctct ggcttgcgag gcgttgcttt ctcgacctta
     4141 atccaatcgc ctgccatttg gcctacatcc ttgaatccct gccaaagcct ggccgtccgc
     4201 tccgctggaa gcagaaggcg gacgaccaga caaaactgcc gcaccttgcg gcagacgcac
     4261 tcgactaaaa cggaatcggt cgctcgccag ccatagccgg ctcggcaggc ggagctggcg
     4321 gggccgaggg acgcccattg ccgttcgtgg ctaccggcgg cacgttgcgc gagagattgc
     4381 cccgtagggc acgcagcgat gcaccaaagc gattctcgag gtcggcggcc ttcgcgccgt
     4441 ccacgttgct catcgcccca ggcgtgcggt cccaggcatt gataaagccg atcttgcgct
     4501 gaacctgatt cttgtacgtc tcctcctcaa tcgtcacttg gcacgccgtc ggctgccacg
     4561 tttcattgac gatgctggag aacgatgcgt cccagccagc gtgcttcatc agggactcgg
     4621 ctgccccgtc gttcgtgttg ccgtcttttt tgacaaccca cacatccccc ctagcctcca
     4681 tctcgtattg agcccagggc tgccactcct ggccgtccca catttccagc agtttgcacc
     4741 gcagggatac cgcgacactg ccagagtcca cttgctttag gccgtactcg acaatctcgg
     4801 cccggaaaat acctttgcga tcaaccggct gcatgacaaa actccttact tagaaaacaa
     4861 caacttccac aaatcaggtt cgccgtcgcg atagatgacc gtgctggata catcccgcga
     4921 tttggccttg tgcgttggca tctcggatgg atagatggtg cgcgttccag cgcccttacc
     4981 tttgccgtcc ttggttacag atacgtcgta gccgacgtag aaaaagtggt cgcaccactc
     5041 aaagacgcgg ctgcgaattg gaccctttgg agtctcttgc atccgatgct caaatctgat
     5101 ccagtcttcc ccgtatgggt ttgggacgga agccttaaag tcgtggcaga tgacgacgac
     5161 gtgcttcccg cgacgaataa cggcgtccag gtccccgagg attttcagga acgtctcgta
     5221 ggcgtgggtg tagcccttgc cgtacccgta gccttcgatc ttgtccacaa actccccgcc
     5281 ctcagtcgga acggttgcga gcgtatgggc gatggcaagc tcctccgcct tggtgaatga
     5341 gtcgatgacg actgcgccat acggggccaa caggctttca tcgtgcagca ctccgcgaag
     5401 ctcgtcccac gtttggatgt cgccgactcg gcttatattg aggaaccggc tgccgttctc
     5461 gacatcgaca atgagtgggt tgatgccgac ttccttgaga agcgacgcga gctttgtttt
     5521 gccgattccg cccgttccat agatgccgac tcgctgggcc cctatcgagc ggccggttgt
     5581 tacggataat ttgcgcgccg gagcgctacc aatagctctg ctctgcgaat tggggcctgc
     5641 tggcggtggc ggtgctgggg gcggtggttt cggcggtgct gcggttgaca tgggttctct
     5701 ccagttctgg gtgacaatca ctaaccaact caaatccttc cggcggcgtg ccgttgaacg
     5761 acgagccgcc gctgcaaatt ccgaagtacg ggcagaatcc gcaagtgttc ttattcaccg
     5821 tgcggaacca ccgattgttt ttctgcgcgt cgcggatgag ctgctggatt tcccacagct
     5881 ccgcctgata ctcgtccagg tcttggtcga gccgcgggac ttcgacgcga gcgaagtagt
     5941 agtccggccg ggcggcgatg tccttcgaca gcttctcgcc ccactcctcc gtcgtcatcg
     6001 gccgcgactg gagaacgtag cccttatcct tgtcgcccgt ctgacgccac tggccgcgtt
     6061 ccgttttgac ccgcgtgccg aattcgttga gcacaatctt tgcgcccaac tcgtccagaa
     6121 ccggcacgtc agtcgcggca atcgtgggct tccgcgtcac gtcgtagagc accgtctgga
     6181 ccggatagcc aagctgcctg gccgcaacca tgtagagact gatctggtgg tcgatgcgga
     6241 gccgacgcca caggtccgaa tcgtgcccga tgtcgtcgcc aagcaacttg gactccttca
     6301 ccgccagccg cccgtcctcc atcttcacga tgccgtcgat tttgccggcc aggttgaaca
     6361 acgtggttcg cttgccggtg gatgggttga ctagcggcaa ctcgaacggc aattcaacgg
     6421 cgacgttctc cagcgggtgt gacgcccacc gccagtcgta ggcgcagaca agccgcagca
     6481 ccgtctcgcg ttcgtagctc cactcggcgg ggttcataaa cactgggcag cgcgtgtact
     6541 tgctacgcac ggcctggcac gcggcgacga gccccagccc cttgccgagc gattcgatac
     6601 cttcgtggaa cgccgagccc atccgcaggg ccttggcgtc gtcgatgcgc cgcaagccga
     6661 gttcgtacgc gaagtagtgt cgcttgcggc acgtcttaaa ggcatcttgg cggctatgtg
     6721 tgagaagctg tttcattggc actccacctt ccgcttcttc acctcgggat gcccctttaa
     6781 gtaactgtcc cgagcctcgg ccgcctcgcg cttcgtgtga tacggccccc agagcggtac
     6841 gtctggcgtc accatcgcgg cgtccagcca ccagccgtcg ggcaattgcc gcagggccag
     6901 gggatagacc cacttgtaaa gttttggtcc gctcatgccg tcctgccttt cttaatccgc
     6961 cgatacttcc gcacttcccg ccgttccccc gccagcagct tcgccgtatc aatcccgtcc
     7021 ggcgctccct tactcgcctg gtcgcgggtc atgtcgatca gctccagacc ctctttccgg
     7081 ctccgctcca tgtcggcgac gattgcggtg gtggcggtgg tcataattcc accctgacct
     7141 tcggccgcga ccgccgacgc tgcctcaata actcccgcac ttccgcgtcg gatttcatct
     7201 ggaacgtcgt gatgatgacg acgcactctt gatcccgcag gcagacgagg cagagcgatt
     7261 cgacggcgca gaagcgataa aacttccctt ctccgtcgct ccagccgcca gtctttttga
     7321 gccaggatat ttgccttgga gtacacggcc gggaaatctc ggctgcgtgc gtcagtcggt
     7381 ccacagcgtc ccggcgcgaa cattgttcga ctcgctgcat gtagcgattg acggcgtgct
     7441 tcgtggggac gaagcgatgc cccgaccgcg cttttgtcgc tgtagttggc atcaaacacc
     7501 cgcctcccgc agtacgcgca tcacggcgtc gtagccaagc tcgtcgttga cggcgaccag
     7561 gccgcggccg aacagagcaa gggccttggc gtcagcgcgc atataggtgc tcccgtagtc
     7621 gcctcgatgg caccgcacaa tcgccccgtg agcgcagaag ca
//
LOCUS       DASUGB010000419         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_324935, whole genome shotgun sequence.
ACCESSION   DASUGB010000419 DASUGB010000000
VERSION     DASUGB010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324935"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..534)
                     /locus_tag="VFV87_08105"
     CDS             complement(208..534)
                     /locus_tag="VFV87_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5103756.1"
                     /translation="MKLPTEIFGDVVVVHTPEELSNDSAGQLERFLTSLERSNVVVDL
                     NGSETIDGSGLEALLAAQEALIALEGNLKISTSNTANRKILEITRLDQQLEVFETVLD
                     AVKSFS"
     gene            complement(602..1009)
                     /locus_tag="VFV87_08110"
     CDS             complement(602..1009)
                     /locus_tag="VFV87_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5103757.1"
                     /translation="MPHRILLCDDELHILRAAEFKFKRAGYDVVCACDGEEGWERVVE
                     RQPDIVVTDCQMPRLNGLQLAQRIRSTPQTSEIPVIMLTGKGFELSPEELREQFGIRC
                     LMAKPFSPRELFARVEAVLAGEEIELAPLALHP"
     gene            complement(1098..2612)
                     /locus_tag="VFV87_08115"
     CDS             complement(1098..2612)
                     /locus_tag="VFV87_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5103758.1"
                     /translation="MPTHFTRMFERRFPHLMPTVILAVVCATLGLAWVIEPSFAPGGW
                     PGRLIVLAAGTTIVTLIAYLNTTRSQQSVEIARQYIDRLCELEPAQLRDEAAFDALPQ
                     REGDRMWREMCQKLRARLAEFGRRADELEMARAGAEVRARRAVSEREQLHDILFGLAD
                     PVLAVDQFGDVVLANPSAERLLVPSSENGEHPALEQLDNFEQLLGMLTDARRRRSPTQ
                     RSGELTVQDAEGHERWFRIACRSLAQQQASTNGEGSSGHGAVAVLTDITGQKAIQKRN
                     AEFVSSVSHEMKAPLSSIRAYVELLADGEAEDDETREEFLGVINSQADRLQRLVENLL
                     NIARIEAGVVAVNKAPLSLNELLQEATSVMQPQAENKQITLTGDLSPLYLGVLADRDM
                     LLQGAINLISNAVKYTPNGGTVTLKSRLNDQEVVFEVCDTGVGLSPEDCQRVFEKFYR
                     VKKDRDMAPGTGLGLALVKHIVEDVHSGRIEVESEVGQGSTFRVILPGVAQLDA"
     gene            complement(2860..>3555)
                     /locus_tag="VFV87_08120"
     CDS             complement(2860..>3555)
                     /locus_tag="VFV87_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5103759.1"
                     /translation="GSVRLSVIIPALNEEASIRRAVESAWLGGAGEVLVADGGSTDET
                     LPIAATLNCHVVSAPRGRASQQNAAARKAAGDVLLFLHADCALASDIGAQIDRALANS
                     NVLHGAFRQRIEAPGLAYRWLEAGNAARVRLFGLPYGDQAIFIRRRTFEELDGFPEEP
                     FLEDLILMERLARRCRPVLLPGPVFVSPRRWLTSGVVRQTLRNWWIVARYRSGASPAD
                     LARSYRGDDVEAK"
BASE COUNT          644 a         1104 c         1120 g          687 t
ORIGIN      
        1 gtttcctagt cgcggcggcg cggtggtgga ggtggacatg gcgactcagg gttcagggct
       61 caggggcggg aagcaattgc aaattgcaaa atgaagattg caaatctcaa attgggcagg
      121 acacgcgagc gttcaatttg cacttttcaa tttgcaactc gcaattttca atccgccggt
      181 cgtttggtcg gcgtctccga aggcctgtca ggaaaagctc ttcaccgcat ccagcacggt
      241 ctcgaatact tcgagctgct ggtccaggcg ggtgatttcg aggattttgc ggttggcggt
      301 gttgctggtc gagatcttca gattgccttc gagggcgatg agcgcctcct gagcggccag
      361 gagcgcttcc agcccgctgc cgtcgatcgt ttcgctgccg ttgaggtcca cgaccacatt
      421 gctccgctcc agcgaggtga ggaatcgctc gagctggccg gcggaatcgt tgctcagctc
      481 ctcgggcgtg tggaccacga ccacgtcgcc gaagatttcg gtgggtagct tcatggccag
      541 gggtcaggag tcaggaacca ggggtcagga accaggggtc agggaccggg agccaaaggg
      601 gttaaggatg aagcgctagg ggagcaagtt cgatttcttc gccagcgagg accgcttcga
      661 cgcgggcgaa cagttcccgc gggctgaatg gcttggccat caggcagcga atgccgaatt
      721 gctcgcgcaa ctcctcgggt gagagctcga agcccttacc ggtgagcatg atcactggaa
      781 tctcgctcgt ttggggcgtg ctgcgaatcc gctgcgcaag ctgtagtcca ttcagccgcg
      841 gcatctggca gtcggtcacg acgatgtccg gctggcgctc gacaacgcgc tcccagcctt
      901 cttcgccgtc acaggcgcag acgacgtcgt acccggctcg tttgaacttg aactcggccg
      961 cacgcaggat gtgcagctcg tcgtcgcata acagaattcg atggggcatg acttgctcct
     1021 ttgtcatttg tcacttgtca ttggtcattt gccatgaggc tctcatcaaa tgacaaatga
     1081 caattgacca atgaccatta cgcatccaac tgggccacgc cgggcaagat cacgcggaac
     1141 gtgctcccct ggccgacctc gctttcgacc tcgatccgtc cgctgtggac gtcttcgacg
     1201 atgtgcttga ccagcgccag ccccagcccg gtgcccggcg ccatgtcgcg atcctttttc
     1261 acgcgataaa acttctcgaa cacgcgctgg caatcttccg gcgaaaggcc cacacccgtg
     1321 tcgcatacct cgaagaccac ttcctggtcg ttaagtcggc ttttgagcgt gaccgtgccg
     1381 ccgttgggcg tgtacttgac cgcgttggaa atcaggttga tcgcgccttg caagagcatg
     1441 tcgcggtcgg ccagcacacc caggtagagc gggctcaggt cgccggtcaa tgtgatttgc
     1501 ttgttctcgg cttgcggctg catgacgctc gtcgcttcct gcaacagctc gttgagcgag
     1561 agcggggcct tattgacagc caccacgccc gcctcaatcc gggcgatgtt cagcaggttt
     1621 tccaccagcc gctgcaggcg gtcggcctgg ctgttgatga cgcccaggaa ttcctcgcgg
     1681 gtctcgtcgt cctcggcttc accgtcggcg agcagctcga cataggcccg aatgctcgac
     1741 aggggcgctt tcatctcgtg gctcaccgac gagacgaatt cggcgtttcg cttctgaatc
     1801 gccttctggc ccgtgatatc ggtcagcacc gcgacggcgc cgtgtccgga tgatccttcg
     1861 ccatttgtgc tcgcttgctg ctgggccaat gagcggcagg cgatgcggaa ccagcgctcg
     1921 tggccttcgg catcttgaac ggtgagttcg ccgctgcgct gcgtgggcga acggcggcgt
     1981 cgggcgtcgg tcagcatgcc caggagctgc tcgaagttgt cgagttgctc caacgcggga
     2041 tgttcgccat tctccgaaga gggcacgagc agccgttccg cgctgggatt ggccagcacg
     2101 acgtcgccga attgatcgac agccaggacc gggtcggcca ggccaaagag gatgtcgtgc
     2161 agttgctcgc gctcgctcac ggcgcgccgg gcgcggacct ccgcccctgc ccgggccatc
     2221 tccagctcgt cggctcgccg gccgaattcc gccagtcgcg cccgcagctt ctggcacatc
     2281 tcgcgccaca tccgatcccc ctcgcgctgc ggcaaggcgt cgaaagcggc ttcgtcgcgc
     2341 agttgggcgg gctccagctc gcacagccgg tcgatgtact gccgggcgat ctcgacggat
     2401 tgctgcgagc gggttgtatt cagatacgcg atcagcgtca caatggttgt gcccgcggcg
     2461 agcacgatca gccgtcccgg ccaacctcct ggcgcaaacg acggctcaat gacccaggcc
     2521 aggccgaggg tcgcacagac gaccgccagg atgacggtcg gcatcaggtg cggaaaccgt
     2581 cgctcgaaca tgcgcgtgaa gtgggttggc aacgctgcac tccgccgcgc gcaggctttg
     2641 gcctccgcgc agcttgagcg cgcggcgtcg gactttgacg ccgtatcacc acaaacctag
     2701 gtcagacgtt ccgaatgtcc aaagcgggag ggcaaaagcg tctgtgacac caatcggagg
     2761 ggaatgtgaa gttggatcgg agagtttcca tggggacttc acacgggcgc taccaggcgc
     2821 ttccgctacc atcgttttaa ccggagaaat cggggctggt tacttagcct ccacatcgtc
     2881 gccgcgatag ctccgcgcca gatcagcggg cgatgcgcct gatcgatagc gggcgacgat
     2941 ccaccagttc cgcagcgttt gccgcacgac gccgctcgtt agccagcggc gcgggctgac
     3001 aaagaccggc cccggaagaa gcacgggcct gcatcgccgc gcaagccgct ccatcaggat
     3061 caagtcttcg agaaatggct cctccggaaa gccgtcaagt tcttcaaacg tgcggcggcg
     3121 gatgaagatc gcctgatctc catacggcag accgaacaac cgcacgcggg cggcattgcc
     3181 cgcttccagc cagcgataag cgaggccggg cgcttcgatt cgctggcgaa aagcaccgtg
     3241 gaggacgttg gagttagcga gcgcacggtc aatttgcgcg ccgatgtcgc ttgccaacgc
     3301 gcaatcggcg tgcagaaaca ggagcacatc acccgcagct ttccgggcgg cggcgttctg
     3361 ttgcgaggca cgaccccgtg gcgcggaaac cacgtgacaa ttgagcgtgg ccgcaatggg
     3421 cagtgtttca tccgtgctgc cgccatcagc gaccagcacc tcgccggcgc cgccgagcca
     3481 ggcggattcc accgcccggc gaatgctcgc ctcttcattg agggcgggga tgatgacgct
     3541 cagtcgcacg ctgcc
//
LOCUS       DASUGB010000420         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_46465,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000420 DASUGB010000000
VERSION     DASUGB010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46465"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFV87_08125"
     CDS             complement(<1..284)
                     /locus_tag="VFV87_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103760.1"
                     /translation="MNQVEILSAMACLVTSLLLYVAAYGLAAWVRPHLQGRLAWLVAL
                     VFPALKLEAWLFEPIAECDLPPKQREFFHAHTPSFIARNYEPLGDFVLLR"
     gene            390..944
                     /locus_tag="VFV87_08130"
     CDS             390..944
                     /locus_tag="VFV87_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103761.1"
                     /translation="MNAYQHAFEAATRDPRYLANLDWGKPRRGHPEGTVRAHIEELEG
                     NLSRFRTRLSDDEYWKLRLLIHTHDSFKAEALSGVPISHPQSHASLARAFLAEFCPDE
                     DLLAMVQLHDEPHALFQQHQRRGSVDAERFARLLAAIRDWNLFSAFLLIDGCTAGKDR
                     GPLQWIFSQIAGRRESSFGIQDIR"
     gene            1046..1966
                     /locus_tag="VFV87_08135"
     CDS             1046..1966
                     /locus_tag="VFV87_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103762.1"
                     /translation="MLGLPGTRARGFTLVELLVVIAMIGLLVALVLPAVQMAREASRR
                     ASCQNNLRQLGISLHNYHDVLQQLPPARIASPAHNWMALTLPFIEEKNVQDIYRYDTD
                     WNAPENQAAIALHIGILTCPSAAGGPKRRDEFATGRFAAVTDYATPAGVNAIVYSANG
                     LTQPGDLRGVINGSQGISLLEIRDGTAHTIIVIEDSGRPAFWLKGRMGPQSTTDGCGN
                     DDVAGGRVSGAGWADPAAALPLHSFAWDGLTCPGPCVMNCTNNNEPYAFHPSGMNSVF
                     ADGSTRFLQQNLDVYAFAAYITREGGEVVD"
     gene            complement(2000..2773)
                     /locus_tag="VFV87_08140"
     CDS             complement(2000..2773)
                     /locus_tag="VFV87_08140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5103763.1"
                     /translation="MIKELFNLSGRTALITGGSKGIGKACARGLAECGANVAISARNE
                     DELKKAKAEIADGLSVKVEYFVSDMLDRAAVKKLAADVLKTFGRCDVLFNNAGSNKPQ
                     ALKDTTEEAWDNIVELNLTSCMLLSRELVPSMIQNKWGRIIYTSSIMGLASNPARGCY
                     SATKAALIGMARAQALELGPHGITVNCLAPGPVATDLPMSILSPEQKKIFAERTAVKR
                     WGETIDMVGPVVMLAAPAGAFITGNVILADGGILCRTFD"
     gene            2936..>3268
                     /locus_tag="VFV87_08145"
     CDS             2936..>3268
                     /locus_tag="VFV87_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103764.1"
                     /translation="MLEEGKGVRAMSKNTASTGLAALGLAALALAGCGTVITGPTKQI
                     RSPEPQTVFTSMPIPGDVKQARYEEPLEPIRAPGLKPFAAWNESDAAADALGRIGPPA
                     VPALAQALH"
BASE COUNT          591 a         1011 c         1067 g          599 t
ORIGIN      
        1 ctgcgcagca ggacaaagtc gcccaagggc tcgtaattcc gcgcgatgaa cgagggcgta
       61 tgggcgtgga agaactcgcg ctgcttgggc gggaggtcgc actcggcgat gggctcgaag
      121 agccaggcct ccagcttgag cgccggaaag acgagggcga ccaaccaggc cagccgccct
      181 tgcaaatgcg gccggaccca ggccgccagg ccatacgctg ccacgtacag cagcagactc
      241 gtaaccagac aggccatcgc actgaggatt tccacctgat tcatagtcca cctcgttcaa
      301 ttggcgcgcg ggcgccttaa gcctagctta cgaatcctgg ccgattcgcg gcgcgaaccc
      361 gccctggaac ctggccatct ctaaccggca tgaacgccta tcagcacgca ttcgaagccg
      421 cgacacgcga tccgcgctat ctggccaatc tcgattgggg aaagccgcgg cgcgggcatc
      481 ccgagggtac ggtccgcgcc cacatcgagg agctcgaagg caacttgagc cgcttccgca
      541 cgcgcctttc ggacgatgag tattggaagc tgcggctgct gattcacacg cacgattcgt
      601 tcaaagccga ggcactttca ggcgtgccga tcagccatcc gcaaagccac gcctcgctcg
      661 cgcgggcctt tctcgccgaa ttctgtccgg acgaggatct gttggcgatg gtccagcttc
      721 acgatgagcc gcatgcgctg tttcagcagc accagcgccg cggcagtgtg gatgccgagc
      781 gattcgcacg cttgctggcc gcgattcgcg actggaacct attctcggcg tttcttctga
      841 tcgacggatg tacggcggga aaagaccgcg ggccgctgca gtggattttc agccagatcg
      901 caggccgccg cgaatcgagc tttggaattc aggacattcg ctgattgccg gcgaatcgaa
      961 cggttgataa gtgccgcgaa tgcgagctac actcaagcgt gacttgtccg ccagcctcaa
     1021 atcgatcgat ggattgaggt ggaaaatgct cgggttaccc ggcacgcgcg cccggggctt
     1081 cacgctcgtc gagctgctcg tcgtgatcgc gatgatcggc ctgttagtgg cgttggtctt
     1141 gccggccgtc caaatggcac gcgaggccag ccgccgggct tcgtgccaga acaacctccg
     1201 gcagctcggg atttccctgc acaactacca cgatgtcctc cagcagcttc cgccggcccg
     1261 cattgcgtct cccgcgcaca attggatggc tctgaccctc ccgtttatcg aggagaagaa
     1321 cgttcaagac atctatcgat atgacacgga ctggaatgca cccgaaaacc aggcggcgat
     1381 cgcgctgcac atcggcatct tgacctgccc ttccgctgcg ggcgggccaa agcggcgcga
     1441 cgagttcgcc actggccgat ttgcggcggt caccgactat gcgactccag ccggcgtgaa
     1501 cgcgatcgtc tattcggcca atggcttgac gcaacctggc gatttgcgtg gcgtcattaa
     1561 cggctcgcag ggcatttcgc tgctcgaaat tcgggatggc acggcgcaca cgatcatcgt
     1621 gatcgaggat agcgggcgac cagcgttctg gctcaagggg cggatggggc cacaaagcac
     1681 caccgatggg tgcggcaacg acgatgtggc aggcgggcgc gtcagcggcg ccggctgggc
     1741 agatcccgcg gctgctttgc cgctacattc gttcgcctgg gatggactca cctgcccggg
     1801 accgtgcgtg atgaactgca cgaacaacaa cgagccgtat gcgttccacc cctccggtat
     1861 gaactctgtg tttgccgatg gcagcacacg gttccttcaa cagaacctgg atgtctatgc
     1921 ctttgcggcc tacatcacgc gcgaaggcgg ggaagtcgtg gactgacatt ccggcggctt
     1981 gcaggaccac cgcacgatct caatcgaacg tccggcagag gatgccgcca tcggcgagga
     2041 tgacgttgcc ggtgatgaaa gcgccggcgg gggcggccag catcacgacg gggccgacca
     2101 tatcgatcgt ttcgccccag cgcttgacgg cggttcgctc ggcgaagatt ttcttctgct
     2161 cgggcgaaag gatgctcatc ggcaggtcgg tagcgaccgg ccccggggcg aggcaattga
     2221 ccgtgatgcc gtgcgggccc agttccagcg cctgggcccg ggccatgccg atcagggcgg
     2281 ctttcgtggc cgaatagcag ccgcgagcgg ggttggaggc caggcccatg atcgacgagg
     2341 tgtagatgat ccggccccac ttgttctgga tcatggaggg gaccagttcg cgcgacagga
     2401 gcatgcagct cgtcaggttc agctcgacga tgttgtccca ggcttcttcg gtagtgtcct
     2461 tcaaagcttg cggcttgttg ctgccggcgt tgttgaagag cacgtcgcag cgaccgaagg
     2521 ttttgagcac gtcggcggcg agctttttga cggcggcccg gtcgagcatg tcggagacga
     2581 agtactcgac cttcaccgac aggccgtcgg cgatctccgc cttggctttt ttcagctcgt
     2641 cctcgttccg ggcgctgatg gcgacgttcg ccccgcactc ggccagcccg cgggcgcagg
     2701 ccttgccaat tcccttgctg ccgccggtga tgagggccgt gcggccggag aggttgaaga
     2761 gttccttgat catggggcag ccctcgcagt cgatcggttg aagcctggat tcaggtctta
     2821 cgctaaacgc ggttttgcgg gctggcaagg gtgtgagggc cgattgggcg gcaggccccg
     2881 cgaaatccgg gcggcaacgc accattctgt atggacactg cgatggatct ggcggatgct
     2941 ggaagaaggt aaaggggtgc gtgcgatgtc gaaaaacact gcgtcgacgg gcttggcggc
     3001 gcttggcctc gcggcgctcg cgctcgccgg ctgtggaacc gtaattacgg ggccgacgaa
     3061 gcagatccgc tcgccggagc cgcagacggt gttcacgtcg atgccgatcc cgggcgacgt
     3121 gaaacaggcg cgctacgaag agccgctgga gccgattcgt gcgccgggcc tgaaaccgtt
     3181 cgccgcctgg aacgagagcg acgccgcggc ggacgccctc ggccgcatcg gtccgcctgc
     3241 cgtgccggcc ctggctcagg cgctccac
//
LOCUS       DASUGB010000421         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1992448, whole genome shotgun sequence.
ACCESSION   DASUGB010000421 DASUGB010000000
VERSION     DASUGB010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992448"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VFV87_08150"
     CDS             complement(<1..121)
                     /locus_tag="VFV87_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5103765.1"
                     /translation="MNRDFNDELLSAYLDGELAPSERSAVENELATNAQARRLL"
     gene            complement(177..761)
                     /locus_tag="VFV87_08155"
     CDS             complement(177..761)
                     /locus_tag="VFV87_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5103766.1"
                     /translation="MAQGDQELILATRRGDSAAFGSLVRKYQDRLLSALFQVSGSRDE
                     AEDVAQEAFVTAYLKLASFAGDSSFYTWLYRIAFNTAISRRRKRRGESSVEHTRELTG
                     GEPLDDCEAAEDRLLRQERAGQVQRALALLPADFRSVLVLREMEGCDYDTIAQILEVP
                     VGTVRSRLHRARLQLKEQLIAVLEGGGNEQRQRR"
     gene            complement(902..2251)
                     /locus_tag="VFV87_08160"
     CDS             complement(902..2251)
                     /locus_tag="VFV87_08160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX5103767.1"
                     /translation="MLRPKRALPFYGRHPWVRDSAVERVEPMSIAGEHFLDLDGQTVD
                     LVNEKGKFIARGFYNSQSRIRVRLYSWSPLEPLDEAFFRGRIEAAIELRRKIGYERGP
                     LAPRADSDLAAGDNVRPASDIAIADSVLSRSERITTDNSLSRSERTTTEAAAAARLVF
                     SEADGLSGLVVDRYGEHLVVQVTSLAIAQRVEMIVAILQDLLQPRGIVLRTEKSMAQI
                     EGVGLAEGKVWGELAEGTLEIVEHGLRYRIDLHEGQKTGFYLDQRENRRAAAAYVRGG
                     KVLDMFCYTGGFAMCAAALGGAADVLGIDTSKKALAQARANAALNGLANVRFETGDGF
                     QTLDRLLGEEARFDAVILDPPKFARNQSGASQALMAYHRLNRAAIGLLPRGGILVTCS
                     CSGGVSREDFLQMLSGVAQKSGRDIQVLDQRGAAPDHPVSATCLQTEYLKCLICRVI"
     gene            3060..>4486
                     /locus_tag="VFV87_08165"
     CDS             3060..>4486
                     /locus_tag="VFV87_08165"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5103768.1"
                     /translation="MPSAHGLSDAAGSSPLIPPGTLPPLGERDLPPPVPIRQMIGPSI
                     ILAGLALGSGEFVLWPYITLRSQFTLFWACLAAVTMQYFLNMEIMRWTLATGESAMTG
                     MIRLSRHLAWIFLVLNVVPWMIPAWATGAAEIVSWLWQAPQYDAAGHVVGQSHVTWIA
                     MGGILLCGVILTAGPVVYETVEKVQFVLVVFIIIVVVVLAGWLVAGRPDALVQQATTT
                     IALGPPSKLASLDEELTPILLLGALAFAGAGGTMNLCQSNYIRDKGYAMGQHIGRITS
                     PLTGHAEPIAEIGSHFPHTPENLRRWRAWFRAAAWEHFASFLLTCIVCLTLLTLICYI
                     LFYDSAGRPIAAAGAFKDNLQFVWAEANMLAGMIGPAARWAFLLMGVAILFTTEFGVL
                     DAASRISTDIVKVAWLRDNPRWSEGRLYFTFLWGEIALACGVLLLERFGFHLGALGLF
                     KLTAAMNGGVMFLYSLALIYMNYFRL"
BASE COUNT          755 a         1514 c         1387 g          830 t
ORIGIN      
        1 ccagcagccg gcgggcctgc gcgttggtcg ccaattcgtt ctcgaccgcc gagcgctcgg
       61 acggcgcaag ctcgccatca aggtaagcgc tgagcagttc gtcgttgaag tcgcggttca
      121 tggctcgtca tccgtcgcta gatccatcct gcccacaacc aacagccgcc atgcgtctat
      181 cgccgctgtc gttgctcgtt tcccccgccc tccaggacgg cgatcagttg ttctttcagt
      241 tgcagccggg cacggtgcag tcggctgcgg acggtgccca cgggcacctc gaggatctgg
      301 gcgatcgtgt cataatcgca gccctccatc tcccgcaaca ccagcaccga ccggaaatcg
      361 gccggcagca gcgccaaggc ccgctgcacc tggccggccc gctcctgccg cagcaggcgg
      421 tcctcggccg cttcgcaatc gtccagcggc tcgccccccg tcaactcgcg ggtgtgctcg
      481 accgagcttt cgccgcgtcg cttccgccgc cggctgatgg ccgtgttgaa ggcgatgcgg
      541 tacagccagg tataaaacga gctgtcccca gcgaatgacg ccagcttgag ataggccgtg
      601 acgaacgcct cctgggcgac gtcctcggcc tcgtcccgag agccggacac ttggaacaaa
      661 gcagagagga gccggtcctg gtactttcgc accaggctgc cgaacgcggc cgagtccccc
      721 cggcgcgtgg caaggatcag ctcctggtcg ccctgtgcca cgttgtcacc gccttagacg
      781 caggggccta tggggaagtt cccagtcgcc gcctagggat tgtattcgca gatgcaaagc
      841 cagttattgc acggagttag aaccgatgcc cactaccctg ccgtcctcaa tccccgctcg
      901 gtcagatcac ccggcaaatc aaacatttca gatactcggt ctgcaggcaa gtcgccgaaa
      961 ccgggtgatc gggcgcggcg ccgcgctggt cgagcacctg gatgtcgcgg cccgatttct
     1021 gggcgacgcc cgagagcatc tgcaggaagt cttcgcgcga cacgcctccc gagcaactgc
     1081 acgttacgag gattccgccc cgcggcaaga ggccgatcgc cgcccggtta agccgatgat
     1141 aggccatcag ggcctggctc gcgccgcttt ggttgcgggc gaatttgggc ggatcgagaa
     1201 tcaccgcgtc aaagcgggcc tcctcgccca gcagccggtc gagcgtctga aagccgtcgc
     1261 cggtttcgaa gcggacgttg gcgaggccat tgagcgcggc gtttgcccgc gcctgggcga
     1321 gagccttctt gctggtgtcg atgccgagca cgtcggcagc tccgccgagg gccgcggcgc
     1381 acatcgcaaa gccgccggtg tagcagaaca tgtcgagcac cttgccgccg cggacgtacg
     1441 cggcagcggc ccggcgattc tcgcgctggt ccaggtaaaa gcctgtcttc tgcccttcgt
     1501 gcaagtcgat ccggtatcgc aggccgtgct cgacgatctc cagcgttccc tcggccagct
     1561 cgccccacac tttgccttcg gccaggccca cgccctcgat ctgggccatg ctcttttcgg
     1621 tccgcagcac gatgccgcgc ggctgcaaca ggtcctgcag gatggcgacg atcatctcga
     1681 cgcgctgggc gatggccagc gaagtgacct gcacaaccag gtgctcgccg tagcgatcga
     1741 ccacgaggcc cgagaggccg tcggcctcgc tgaacacgag ccgcgcggcg gcagccgctt
     1801 ctgtcgtagt cctctcgctc cgcgagaggc tattgtccgt tgtgatcctc tcgctccgcg
     1861 agaggacact gtcagcgatg gcgatgtcgc tggccggccg aacattgtcc ccggctgcca
     1921 ggtcactatc cgctcgcgga gcgagcggac cacgttcgta cccaatcttc cgccgcagct
     1981 cgatcgccgc ctcgatccgc ccgcgaaaaa acgcctcgtc cagaggctcc agcggcgacc
     2041 acgaatagag ccgcacgcga atgcggctct gcgagttgta aaacccacgg gcaatgaact
     2101 tgcccttctc gttgaccaga tcgaccgttt gcccgtccag atcgagaaag tgctcgccgg
     2161 caatgctcat cggctcgacc cgctcgaccg ccgagtcgcg cacccacgga tgccgcccat
     2221 aaaacggcaa cgcccgtttc ggccgcagca cgacccgggc cgcggaaagg aggggtgcgg
     2281 aaccttgtac acttttagtg tcaacgatcg acatagccct tcatcatacc ggaagcatgc
     2341 gccagattcc cgactgtcca ttccaacgcc gggcaaggac gctctttcgc gaattgagaa
     2401 ttgacggcag atcgcgcgct gcgtggagca cgcgcacaac ttccattccc gcaacgtgcg
     2461 gcaagcaaaa gatcaagtga ttcggaatgc cagggcaatt gcgccatttt tgggcaatcg
     2521 ggcataaccg ggcacattat tgacccctga cccgcatttc ttgtgaccat cttgacagta
     2581 gtgtaaaaac atgtacaatt cggatgctcg ccagtcgggc ctccgggccg gattgccggc
     2641 ggctctttga caactcgtgc gggtgaattc ggcgggctgc ggttaccgag ttggaattgc
     2701 cgagctgcgg ttcgagtccg acgagatggg caagcccacg ctgtggcgcg gttgcaccga
     2761 ccagcgagaa gggcgcggtg gttaacgggt tccccccgtt tcttcgattt tgttaacctg
     2821 cggggggccg ggatgctgga aaacgcggca tttttcgccg gccaggttga cccgcgaggc
     2881 cagcccgcgt taacgggctg gaagtcgggg gcgggcaaag agttccggtc gccgagcggg
     2941 cgcttcctgg cttgccgtcg tgttaaccag cgatcattcg accagcccga tcttttagga
     3001 caaggcgggc gcttcagcca ccggcgacag caaggccaat tgcggtatca tcagccacca
     3061 tgccctcggc ccatggactc agcgacgctg ccggctcatc gccgctcatt ccccccggca
     3121 cgctgccgcc gctcggcgag cgcgacctgc ctccgccagt tccgatccgc cagatgattg
     3181 gcccgagcat catcctggcg ggactggccc tgggaagcgg cgaattcgtc ctctggccgt
     3241 acatcacgct gcggagccag ttcacgctgt tctgggcctg cctggccgcg gtcacgatgc
     3301 agtatttcct gaacatggaa atcatgcgct ggaccctggc caccggcgaa agcgccatga
     3361 ccggcatgat ccgcctcagc cgccacctgg cctggatctt cctcgttctg aacgtcgttc
     3421 cctggatgat tcccgcctgg gcgaccgggg cggctgaaat cgtgagctgg ctgtggcaag
     3481 cgccgcagta cgatgccgcg ggccatgtgg tgggccagtc gcacgtcact tggatcgcga
     3541 tggggggcat cctgctgtgc ggcgtcatcc tgactgccgg gccagtcgtc tatgaaacgg
     3601 tcgagaaggt gcaattcgtg ctggtggtgt tcatcatcat cgtcgtcgtc gtcctggcgg
     3661 gatggctggt cgccggccgg cccgatgccc tggtgcagca ggctaccacg acgatcgcgc
     3721 tcggaccgcc gtccaagctg gcgtcgctcg acgaggagct gacgccgatc ctgctcttgg
     3781 gagcgctggc ctttgccggc gcggggggga caatgaacct ttgccagagc aactacattc
     3841 gcgacaaggg ctacgcgatg ggccagcaca tcggccgcat caccagcccg ctcaccggcc
     3901 atgccgagcc gatcgccgag atcggctcgc actttccgca cacgcccgaa aacctccgcc
     3961 gctggcgggc ctggttccgg gccgccgcct gggagcactt tgccagcttc ctgctgacgt
     4021 gcatcgtctg cctcacgctg ctgacgctga tctgctacat cctgttctac gattccgcag
     4081 gacgccccat cgccgcggcc ggcgcgttca aggacaacct gcaattcgtc tgggccgagg
     4141 cgaacatgct cgcgggaatg atcggcccgg ccgcccgctg ggcttttttg ctcatgggcg
     4201 tcgccatcct cttcaccacc gaattcggcg tcctggacgc cgccagccgc atctcgaccg
     4261 acatcgtcaa ggtcgcctgg ctgcgcgaca acccgcggtg gagcgagggg cggctgtact
     4321 tcaccttcct gtggggcgaa atcgccctgg cctgcggcgt cctgctcttg gagcggtttg
     4381 gtttccacct cggcgccctg ggcctgttca agctgaccgc ggccatgaac ggcggcgtga
     4441 tgtttctgta tagcctggcg ctgatctaca tgaactattt ccggct
//
LOCUS       DASUGB010000422         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1297370, whole genome shotgun sequence.
ACCESSION   DASUGB010000422 DASUGB010000000
VERSION     DASUGB010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297370"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFV87_08170"
     CDS             complement(<1..236)
                     /locus_tag="VFV87_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX5103769.1"
                     /translation="MAAWWVFKSPSQPRVPNPAAVQIAEIYSSRQGEGLLTGTPSVFV
                     RASGCNLRCWFCDTPYTSWEPEGKDWSVEEIVADV"
     gene            330..698
                     /gene="queF"
                     /locus_tag="VFV87_08175"
     CDS             330..698
                     /gene="queF"
                     /locus_tag="VFV87_08175"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5103770.1"
                     /translation="MAATPTDPRELLETFDNQFPQRDYIVEIVCPEFTSVCPKTGQPD
                     FGTLTITYTPDRKCVELKSLKMYLQSFRNQGIFYENVTNRIADDLVAVLAPRRLKLVA
                     AFTARGGISTSVTVTQEAGG"
     gene            770..1936
                     /gene="larC"
                     /locus_tag="VFV87_08180"
     CDS             770..1936
                     /gene="larC"
                     /locus_tag="VFV87_08180"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX5103771.1"
                     /translation="MKLAYLDCPSGISGDMTLGALVDCGIELAAIQTGIDSLGFPNIK
                     LTSEEVKRKGFRACKVKVHHEPEHAHRHLHHITEKIDASTVLTPPQKDLAKRIFTCIG
                     EAEAKVHGTTLRKVHFHEVGAVDSIADIVGSAVGLSLLGVDRIVCSPVPTGTGYIEIE
                     HGRVSVPAPATAEILKGVPLAPSVCPFELTTPTGAAIVKTVADEFGPLPPMTIQTIGQ
                     GAGDRDLKEQANILRLILGEAGNGTVSDQVWVLETNLDDISGEIIGHCTARLFEAGAL
                     DVYTTSIQMKKNRPGVLLSVLCGAELISKLEKIIFQETSTLGIRRWQACRHKLERRPH
                     TVETALGPIEGKLAVLPDGSTSFSPEFEACRRIAGEKNVPLKDVYEAAMRAFEV"
     gene            2056..>2703
                     /locus_tag="VFV87_08185"
     CDS             2056..>2703
                     /locus_tag="VFV87_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103772.1"
                     /translation="MENDPTLAHSRPAPDSALDGLAAEFASRCAAGDRPQIHDFLHRT
                     SPERRRELFARLLRLELELQIQSGETFAVRAYRQQFAEFADVVEEVLVHFSSFGSSAG
                     KSRQTGVSTVGASQSTEGNTTPPKLSSPPSRQKPAAGSSPLTQFGDYEIQSEIARGGM
                     GVVYKARQIKLNRVVALKMILAGQFATGEDVQRFYSEAEAAAKLDHPGIVPISEVD"
BASE COUNT          540 a          880 c          816 g          467 t
ORIGIN      
        1 acgtcggcca cgatctcctc caccgaccaa tccttcccct ccggctccca ggaggtgtag
       61 ggcgtatcgc agaaccagca gcgcaaattg cagccgctgg cgcgcacgaa aacgctgggc
      121 gtgccagtga gcaggccttc gccctggcgc gagctgtaga tttcggcgat ctgcacggca
      181 gcggggttcg ggactcgggg ctgggagggc gatttgaaga cccaccaagc ggccatcaat
      241 ctaatcatat tcaacctgtc gctgttctac ttcccttccc gaaccccgag cccctagccc
      301 cgaaccccgc gctccgccag aatcgaagca tggccgccac acccaccgac ccgcgcgagc
      361 ttttggagac gtttgacaac cagtttccgc agcgggacta tatcgtcgag atcgtttgcc
      421 cggagttcac gtcggtctgc cccaagacgg gccagccgga cttcgggacg ctgacgatta
      481 cctacacgcc cgaccgcaaa tgcgtggagc tgaagagcct gaagatgtac ctgcagagct
      541 tccgcaacca gggcatcttc tacgagaatg ttaccaatcg tatcgccgac gacctggttg
      601 cggtgctggc gccgcggcgg ctgaagctgg tggcggcgtt tacagcgcgg ggcgggatca
      661 gcaccagcgt caccgtgacg caagaggcgg gcgggtgagt ggtgagtggt gagtggtgag
      721 tggtatgatc ctaagcacca ttcacgactc accattcacc tcccgccaca tgaagctcgc
      781 ctacttggac tgcccctccg gcatcagcgg agacatgacg ctgggggccc tcgtcgattg
      841 cggcattgaa ctcgccgcga tccagaccgg aatcgattcg ctgggctttc cgaacatcaa
      901 gttgaccagt gaagaggtga agcgcaaggg ctttcgggcg tgcaaggtga aagtccatca
      961 tgagccggag cacgcccacc ggcacttgca tcacatcacc gaaaagatcg acgccagcac
     1021 ggtgctcacg ccgccgcaaa aggacctcgc caagcggatc ttcacttgca tcggcgaggc
     1081 ggaggccaaa gtccacggca cgacgcttcg caaggtccac tttcacgaag tcggggccgt
     1141 cgattcgatc gccgatatcg tgggcagcgc cgtggggctt tcgctgctcg gcgtggatcg
     1201 gatcgtctgc tcacccgtcc cgaccggaac cggctacatc gagatcgagc acggccgcgt
     1261 cagcgttccc gcaccggcga ccgctgaaat cctcaaaggc gttccactgg cgccctccgt
     1321 atgtccgttt gagttgacca cgcccaccgg tgcggcgatc gtcaagactg tggccgacga
     1381 attcggcccg ctgccgccga tgaccatcca gacgatcggc cagggcgccg gcgaccgcga
     1441 cctgaaggag caggcaaaca tcctgcggct catcctgggc gaagcgggca atgggaccgt
     1501 cagcgaccag gtttgggtcc tggaaaccaa tctcgacgac atcagcggcg agatcatcgg
     1561 ccactgcacg gccaggctct tcgaagccgg agcgctcgac gtctatacga cgagcatcca
     1621 gatgaagaag aatcggcccg gcgtgctgct ctcggtcctg tgtggcgccg agttgatatc
     1681 gaaactcgaa aagatcattt tccaggagac cagcacgctc ggcatccgcc gctggcaagc
     1741 ctgtcgccac aaactcgagc gccggccgca cacggtcgaa acggccctcg gaccaatcga
     1801 ggggaagctg gccgtcctgc cggacggcag cacgagcttt tccccggaat tcgaagcctg
     1861 ccgccgaatc gcgggcgaaa agaacgtccc gctcaaggac gtctatgaag cggcgatgcg
     1921 ggctttcgag gtctaggcaa ctagccttcc gtccacggat tggcaatgac agtcaaagtt
     1981 tgcgggttgc cgcaaggccg ggttagaatt gccgcttcac gggcccttca cccagtgtgc
     2041 cgggaacgat ggcggatgga aaacgacccc actctcgcgc acagcagacc tgcgcccgat
     2101 tccgcgctcg atggcctcgc ggccgaattt gcctcccgct gcgcagcggg cgaccgcccc
     2161 cagattcacg acttcctgca ccgaacttcg cccgagcggc gacgcgaact cttcgcccgt
     2221 ctcttgcggc tggaactgga gttgcagatt caatcgggcg agacctttgc cgtccgcgcc
     2281 tatcgccagc agtttgccga attcgccgat gtggtcgaag aagtcctggt gcatttctcc
     2341 agtttcggca gttcggcagg caagtcccgc cagaccggcg tttcgaccgt tggggcttcg
     2401 caatcgacgg aagggaacac aacgccgcca aagctctcga gtcccccttc ccggcaaaag
     2461 cccgctgccg gctcttcgcc gctgacgcaa tttggcgact acgaaatcca gagcgaaatc
     2521 gcccgcgggg gaatgggcgt ggtctataag gcccggcaga tcaagctgaa ccgcgtcgtc
     2581 gccttgaaga tgatcctcgc cggccaattc gccaccggcg aggacgtgca gcggttctac
     2641 agcgaagccg aagccgcggc caagctcgat catccgggga tcgtgccgat ttccgaagtc
     2701 gat
//
LOCUS       DASUGB010000423         8396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_325776, whole genome shotgun sequence.
ACCESSION   DASUGB010000423 DASUGB010000000
VERSION     DASUGB010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325776"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFV87_08190"
     CDS             complement(<1..824)
                     /locus_tag="VFV87_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103773.1"
                     /translation="MRGIDVPGLALINHALRAAGVLFVLWFCAGAAPAADRLILRNLD
                     IFTDLTVTAIDEDGVVVTPARQGHSERITWDEIERGMVALDQSRFDELLRELGPPLYR
                     IRQRLKIGDYESLSTPAELLFPRYAERQSATAYMVCQATMWSRLASGQREAAVEPFFR
                     CFELLRAGAARGRALPGNRHMQVDSQTTISPDLLPVWFDAEAAKAALPGVQQAIRGIS
                     QPRPEGVYLYYASLALAADERVEFERVLPSIRGADAQAAVWRDVLLAQREVLGGTSG"
     gene            complement(972..1262)
                     /locus_tag="VFV87_08195"
     CDS             complement(972..1262)
                     /locus_tag="VFV87_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103774.1"
                     /translation="MVKYLALAVALVASLVIVDMAQAGRRCHGGCCGGGCYAGGCPGG
                     VCPAPVAPAPVPAAAVTNAPPTVVVNQAAPVASPAPVYYTSAPRRGLFGWRR"
     gene            complement(1488..3242)
                     /locus_tag="VFV87_08200"
     CDS             complement(1488..3242)
                     /locus_tag="VFV87_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5103775.1"
                     /translation="MSSVDREQQIAQAEEILGDRREQPGFVKGLMFGEYFADRLPAYP
                     SVARDESLTARVQELQKFCTESIDPVAIDKKAEIPQSVISGLGRLGVLGACLPKSVGG
                     LDLSQAAYCRLLEVLGGHCAGTALFVNAHHSIGPRAIVLFGTAAQQQQYLPKLASGEW
                     ISAFALTEPEAGSDAANVQSTATPTPDGKGFQINGQKRWITNGGIAQVLTLMARTPAS
                     GGGESKITAFLVTPDMPGFQVVEKRMEKMGVRGTATSRLAFNNMFVPRQNVLGQMGKG
                     LKVALTVLDFGRTTFGAVCTGAAKFCLARAIEHSKSRVQFGEPLASFELVKEKLAYMQ
                     AGVFAMEACTYETAALIDAGEGDFMLETAMLKVFSTETLWKILNDTFQLHGGLAYFTD
                     RPFERMVRDHRINTIGEGANDVLRAFVALVGMRDVGLELQGVRDAIYQPLGNLTRLGS
                     FAGRKLGSLLVAPAVPVRNGELEPDAAELGKLIRLLGSNVQRLLIQHQMSIVDRQYLL
                     SRVADAATELYVSACVLRRLDAMLRDHHDHAALRLDLESGRYYLLTARRRIRQALSSL
                     WDNDDAQSTALANRLLRS"
     gene            3623..4366
                     /locus_tag="VFV87_08205"
     CDS             3623..4366
                     /locus_tag="VFV87_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5103776.1"
                     /translation="MARKENIDRLLRDWPYDPEGISVRKKKGDDGRDVLQMRLDLGLL
                     QLEATGRPDGQRPEGAESFYDLLVGKSVASGNGYMMTEDECEEADREFMQYYHRRVCW
                     LALREFHNAVADADHTLGLMDFCRDHSPDEQWTLSHEQYRPFVLFHRSQAAALAALGD
                     RGAEAAVEEINKGLDRMRHVFVEYEAEEEFDEDELVQRLTELRESLRQHFRVGRTLQE
                     QLAEAIADEKYELAAEIRDELNRRDSRAR"
     gene            4681..6345
                     /locus_tag="VFV87_08210"
     CDS             4681..6345
                     /locus_tag="VFV87_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5103777.1"
                     /translation="MYVLENPVMQRELLVNLRMGRAFVLLLLYQALLGAVVYFAWPQD
                     TRLDLTGASVEQTKQTKQLVNMFFAGQFVLASMMAPSFASGSITGEKERKTYEMLLAS
                     PLRPAAIVLGKLVASLTHLGVLIFASLPIVMLCLPLGGVSIYEVFAAYLGLILSVLTF
                     GMISVACSAYFQRTSAALVVSYIIILPLALLGAIVWLQLADQGIFRLLLILTVLPAVA
                     MAVCIALFFNTSALLLHPPDVGSEGKEVVDLEQEAQQAVGLVIQRDQFPDRLFAPPKR
                     DDLMEDDANPVYDKEIRSEIFSQGTLMLRLVIQVSMLLAIPFMAVCLYIWPQYAPWYI
                     SYVVMFNMLVGPVFSAGSVTSERERETLDLLLTTVITPWQILWGKLIAGLRVSSVLTL
                     FLVWPVLLACVMVSLYWSNLLSVIAYLSIVLITCLTTAMLALFFSVLFKKTSQAMMTT
                     YLAIIILFCAPLAMNFFAQTFFRDHPNTPFIEQLGMLSPFSAAFAVPLDMGLTAAEAG
                     STAAAEQQALGDWPLFLGYVVLTLGLNGMLLLVMMWLFNTRWRVAG"
     gene            complement(6359..7786)
                     /locus_tag="VFV87_08215"
     CDS             complement(6359..7786)
                     /locus_tag="VFV87_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5103778.1"
                     /translation="MLRSYLQLVAIGLAVGLLASATRTSAAERPNFVLLMADDQGWGD
                     VAYNGNADLKTPVLDEMARTALRLERFYAAAPVCSPTRGSVLTGRHPNRFGCFSWGYM
                     LRPEEITLAEVLKEAGYATGHFGKWHLGPVAADVPNSPGTSGFDQWFSSPNFYENSPL
                     MSANGKVVKTDGEGSQVTVAAALEFIRAAANSERPFLAVVWFGSPHLPHQALEADREP
                     YASHPPAMQNYWGEITAMDRAIGKLREELRKLDIADNTLVWYTSDNGATTPGSTGGLR
                     GKKGSVWEGGLRVPAIVEWPARIKSPRTSSLPAGTVDILPTVLELAGASVPKDRPLDG
                     VSLKPLIDGQMTRRDQSLGFWVYPAPGKPVRSQEILAKLARVQAGEADAEPEFEEPSQ
                     TRYSETELPGHAAWIDGDFKLHRLPGKAGEFRYELYDLAADAKEETDLAAKHAGRVAA
                     MKQQLADWQQSVVQSLNREDYGEPK"
     gene            complement(7841..>8396)
                     /locus_tag="VFV87_08220"
     CDS             complement(7841..>8396)
                     /locus_tag="VFV87_08220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX5103779.1"
                     /translation="LRADSPKSKVQGPNLVGDVGTGSGAIAIAVAKHAPAVDITAIDS
                     SPAALEIAKANAAQHGVSERIEFLEGDLLASLPPEPRFAVIASNPPYVSEAEFEQLAP
                     EVRNHEPRQALVAGLAGTEVIERLIPQAAERLLPGGWLIVELSPMIASRVVDLIAADT
                     RYEPATLLKDLAGLPRVVKARRKT"
BASE COUNT         1498 a         2736 c         2609 g         1553 t
ORIGIN      
        1 ccggaagtcc caccgagcac ctcccgctgc gcgagcagca cgtcgcgcca aactgccgcc
       61 tgggcatctg caccgcgaat agaaggcagc acccgttcga attcgacgcg ttcgtccgca
      121 gccaacgcga gcgaagcgta atagagatag accccctcgg gtcgcggctg cgatatgcct
      181 cgaatcgcct gctgcacgcc gggcaatgcg gccttcgcgg cttcagcatc gaaccagacg
      241 ggcagcaaat ccggcgaaat ggtggtctgc gaatcgactt gcatgtggcg attgccgggg
      301 agcgcgcgac cgcgggcagc gcccgcccgc agcagctcga aacaccggaa aaagggctcg
      361 acagcggctt cccgctggcc cgacgccagg cgcgaccaca tcgtcgcctg gcagaccatg
      421 taggccgtgg cgctttgccg ctcggcatat ctcggaaaga gcagctcggc tggcgtggaa
      481 agcgattcgt aatcgccgat cttcagccgc tggcggatgc gatacagtgg cggaccgagc
      541 tctctgagca attcatcgaa ccgcgactgg tccagagcca ccattccccg ttcgatttcg
      601 tcccaggtga ttctctcgct atggccctgg cgtgccggag tcacgacgac cccgtcttcg
      661 tcgattgccg tgactgtgag gtcggtgaaa atgtctaagt tgcgcagaat cagccggtct
      721 gctgccggcg cagcgcccgc gcagaaccac aggacgaaca gcactcccgc ggcccgaagc
      781 gcgtggttga ttagagcaag acccggcacg tcgatccccc gcatggccgc tggctgatat
      841 ccatcctaga cgagcgccgg cgagggtcaa gcgaaagcag gatacgcttt tttaaatgcc
      901 agagggcacg aaaaaaggag agacgatccc tcgctctccc ttgtgcaatc agaaacacat
      961 cgccgaggcg tttagcgccg ccagccaaac aaaccgcgac gcggagcgct ggtgtagtac
     1021 accggagccg ggctggcgac cggagccgcc tggttgacta ccaccgtcgg cggagcgttc
     1081 gtcaccgccg cagcgggaac cggagccgga gccacaggcg ccgggcagac gccgccgggg
     1141 caacctcccg catagcaacc tccgccgcag cagccgccgt ggcaacgccg accagcttgg
     1201 gccatgtcca caatcaccag gctcgcgacg agagccacag ccaacgcgag atacttgacc
     1261 atttccaatc actccttagc acaggtctgg aagaaacaaa caccggtgga tgggtgaatg
     1321 tagagattgc ggaataagcg tcaagtaggt caacagtacc tattgggaaa tatggataaa
     1381 aagtagtgcg taaccaaagt aagtgcgtaa cacatatggc gttactagct tttaatcacg
     1441 gaccgcattt caccttgtac cgactaaact taggacgatt cacgacctca gcttcgcagc
     1501 aaccggttgg ctaatgcagt ggattgagcg tcgtcattat cccaaagtga cgagagggcc
     1561 tggcggatgc ggcggcgggc ggtaaggagg tagtaacggc ccgattcgag atccagccgc
     1621 aaggcggcgt ggtcgtggtg gtcgcggagc atcgcgtcca gccgcctcag cacgcaagcg
     1681 ctgacgtaca gctcggtcgc ggcgtcggcg acgcggctga gcagatactg ccgatcgacg
     1741 atcgacatct gatgctgaat caacaacctt tgcacgttgg aacccagcag ccgaatcagc
     1801 ttgcccagct cggccgcgtc gggctccaat tcgccattgc ggacgggaac cgccggcgcg
     1861 accaggagcg agccgagctt gcggccggca aagctgccga ggcgcgtcag gttgcccagg
     1921 ggctggtaga tagcatcgcg gaccccttgc agctccaggc cgacgtcgcg catgccgacc
     1981 agcgcgacga acgcccgcag cacgtcattg gccccttcac cgatcgtgtt gatgcgatgg
     2041 tcgcggacca tccgctcgaa gggacgatcg gtaaaataag ccaatccgcc gtggagctga
     2101 aacgtgtcat tgagaatctt ccacagcgtc tcggtcgaaa agaccttgag catcgctgtc
     2161 tccagcatga aatcgccttc gccggcgtcg atcagggcgg ccgtctcgta cgtgcaggct
     2221 tccatcgcga aaacgccggc ctgcatgtag gcgagcttct ccttcaccag ctcgaagctg
     2281 gccagcggct cgccgaattg cacgcgagat ttcgagtgct cgatggcgcg agccaggcag
     2341 aacttcgccg cgcccgtgca gacggcgccg aaggtcgtgc ggccgaaatc gagcaccgtc
     2401 agggccacct tcagcccctt gcccatctgg cccagcacgt tctggcgcgg gacgaacatg
     2461 ttgttgaacg ccaggcggct ggtggcggtg ccgcggacgc ccatcttctc catccgcttc
     2521 tcgacgacct gaaaacccgg catatcgggc gtgacgagaa atgcggtgat cttgctctcg
     2581 ccgccgccgg acgcaggcgt gcgggccatc aaggtgagca cctgggcgat gccgccattg
     2641 gtgatccacc gcttttgccc gttgatctga aagcccttgc cgtcgggcgt cggcgtcgcg
     2701 gtcgattgga cgttggccgc gtcgctgccg gcctcgggct cggtgagggc gaaggcgctg
     2761 atccattcgc cgctggcaag cttgggcagg tactgctgct gctgcgcggc cgtgccaaag
     2821 aggacgatcg cccgcgggcc aatcgaatgg tgcgcattca cgaacagcgc ggtccccgcg
     2881 caatggccgc ccagcacctc cagcaggcgg caatacgcag cttgcgagag gtccagtccg
     2941 ccgaccgatt tcggcaggca cgcgccgagg acgccgagcc ttcctaagcc gctgattacc
     3001 gactgcggta tttcggcctt cttgtcgatc gccaccggat cgatgctctc ggtgcaaaac
     3061 ttttgcagct cttggacgcg ggcggtcagc gactcgtcgc gagccacgct gggatacgcc
     3121 ggcagccggt cggcgaaata ttcgccgaac atcagcccct tgacgaagcc tggttgctcg
     3181 cggcggtcgc cgaggatttc ctcggcctgc gcgatttgct gctcacgatc gacggatgac
     3241 atgcgataag ctgcctggga agtgctggtc aggtaggcgc tccgatagta acccgaagcg
     3301 caagcgaggg agccgcctgc aggcgtattg gcgaggcagt ccctcgctta cgcttcgggt
     3361 tactatgccg ctcgccactg catcttatgc atcggcatcc ggaccggcga atcggtctgc
     3421 aagcgactct ggtggcacgt caacttgtct gccgatgccg caaggtttcc ccagtcggcc
     3481 ccgtcaagcg gcaaattgcg aaaacccggc aaagctaaca atcgaacctg cacaggccga
     3541 aatgcggggt gtataatcga gcgctggcac atgccaattg gtttattggt ttgttgcaac
     3601 agcccgctct cgcgggaacg ccatggcacg gaaagagaac atcgatcgcc tgcttcgcga
     3661 ctggccctat gatcccgagg gcatcagcgt ccggaaaaag aagggggacg acggccgcga
     3721 cgtgctgcag atgcggctgg acctgggcct cttgcagctt gaagccaccg gccgccccga
     3781 cggccagcgg ccggagggag ccgaatcgtt ctacgatctg ctcgtcggca aatccgtcgc
     3841 cagcggcaac ggctatatga tgaccgagga cgagtgcgag gaggccgacc gcgagtttat
     3901 gcagtactat catcgccgtg tctgctggtt ggcgctgcgc gagtttcaca atgcggtggc
     3961 cgacgccgac cacacgctgg gcttgatgga tttctgccgc gaccactcgc ccgacgagca
     4021 gtggaccctc tcgcacgagc agtaccgccc cttcgtcctg tttcaccgct cgcaggccgc
     4081 cgcgcttgcc gctcttggcg accgcggcgc cgaagccgcc gtcgaggaga tcaacaaggg
     4141 cctcgatcgc atgcgccatg tgttcgtcga atacgaggcg gaggaggagt tcgacgaaga
     4201 cgagctcgtc cagcggctga cggagctccg tgaatcgctg cggcagcact tccgcgtcgg
     4261 ccgcacgctg caagagcagc tcgccgaagc gatcgccgac gagaaatacg agctggccgc
     4321 ggaaattcgc gacgaactga accggcgcga ttcgcgagcg cggtagtcgt ctgtatctca
     4381 acgcccagtc ccagggccga aaccggcgtt tctgtatgtc caggttcctc cgctggccgg
     4441 gaggcgatgg cgagggcggc ctctttcaaa gccgccgagc gtcgagtgcg ttccattcct
     4501 tctccgtcta tttcccgcgg ggcatgtgag cgcttccgag tgaatcgaca agaacctact
     4561 ccgcgacact cgacctgccc aatttcaaaa gcggttcgac ttggatgttt gccacagaca
     4621 attcgactat gatcgactgc tgcgattccg cattccgaaa tccgcccttc gcatttcccc
     4681 atgtacgtcc tcgaaaaccc cgtcatgcag cgcgagctgc tcgtcaacct gcggatgggg
     4741 cgggcgtttg tcctgctgct gctctatcag gccttgctcg gcgcggtcgt ctatttcgcc
     4801 tggccgcagg acacgcggct ggacctgacc ggggcgagcg tcgagcagac caagcagacc
     4861 aagcagctcg tcaacatgtt cttcgcgggc cagttcgtcc tggcgtcgat gatggccccc
     4921 agcttcgcca gcggttcgat caccggggaa aaggaacgca agacctacga aatgctgctg
     4981 gccagcccgc tgcggccggc ggccatcgtc ctgggcaagc tcgtcgcctc gctcacgcac
     5041 ctgggcgtgc tgatcttcgc ctcgctgccg atcgtcatgc tctgcctgcc gctcggcggt
     5101 gtatccatct acgaagtgtt cgccgcgtat ctggggctga ttctttcggt cctcacgttc
     5161 ggcatgatca gcgtggcgtg cagtgcgtat ttccaacgca ccagcgccgc cctcgtcgtc
     5221 tcgtacatca tcatcctgcc gctggcgctc ctcggtgcaa ttgtctggct gcagcttgcc
     5281 gaccagggca ttttccggct gttgctcatc ctgaccgtcc tgccggcggt cgcgatggcc
     5341 gtctgcatcg cgctattctt caacaccagc gcgctgctgt tgcatccgcc cgacgtcggc
     5401 agcgaaggga aagaggtcgt cgatctggaa caggaagccc agcaagcggt gggcctggtc
     5461 attcagcgcg accagttccc cgaccgcttg ttcgcgcctc ccaagcgcga cgacctgatg
     5521 gaggacgacg ccaaccccgt ctatgacaag gaaatccgca gcgaaatctt cagccaaggg
     5581 acgctgatgc tccggctggt gatccaggtg agcatgcttt tggcgattcc cttcatggcg
     5641 gtctgccttt acatctggcc gcagtacgcc ccgtggtaca tcagctacgt cgtgatgttc
     5701 aacatgctcg tcgggccggt tttctcggcc ggcagcgtga ccagcgagcg cgagcgggaa
     5761 acactcgacc tgctgttgac caccgtcatc acgccgtggc aaatcctgtg gggcaagctg
     5821 attgccggcc tccgcgtctc gagcgtgctg acgctgttcc tcgtctggcc ggtactcctg
     5881 gcgtgcgtga tggtctcgct gtactggtcg aacctgctat cggtgatcgc ctacctgtcg
     5941 atcgtgctca tcacctgcct gacgacggcc atgctggccc tgttcttctc ggtgctgttt
     6001 aagaagacct cgcaggcgat gatgacgacc tacctggcaa tcatcatcct gttctgtgct
     6061 cccctggcga tgaacttctt cgcccagacg ttctttcgcg accaccccaa cacgcccttc
     6121 atcgagcagc ttggcatgct cagcccgttt tctgccgcgt tcgccgtccc gctcgacatg
     6181 ggcctcaccg cagccgaagc cggcagcacc gccgccgccg agcagcaggc cctgggggac
     6241 tggccgctgt ttttgggcta cgtggtactg actctgggcc tcaacggcat gctcctcctg
     6301 gtcatgatgt ggctcttcaa cactcgctgg cgcgtggcgg ggtgagaccg gcaccgcctt
     6361 acttcggctc gccgtagtcc tcgcggttca ggctctggac gactgactgc tgccagtccg
     6421 cgagctgctg tttcatcgcc gcgacgcggc cggcgtgctt cgcagccagg tcggtttcct
     6481 cctttgcgtc ggccgccagg tcgtacagct cgtagcggaa ctcgccggct tttcctggca
     6541 ggcggtggag cttgaaatcg ccgtcgatcc aggcggcgtg gccgggcagt tcggtttcgg
     6601 agtacctggt ttgcgacggc tcctcgaatt ccggctccgc gtcggcttcc ccagcttgca
     6661 cccgcgcaag cttcgccaga atctcctggc tgcggaccgg cttgccgggc gcgggataga
     6721 cccagaagcc cagcgactgg tcgcgtctgg tcatttggcc gtcgatcagc ggtttcaggc
     6781 tgacgccgtc gagcggccgg tcctttggca cgctcgcgcc ggccagttcc agcacggtcg
     6841 gcagaatatc gaccgtgcct gcgggcaggc tgctggtgcg gggcgatttg atccgggccg
     6901 gccactcgac gatcgccggc acgcggaggc ccccttccca cactgagccc ttcttccctc
     6961 gcaagccgcc cgtcgagccg ggcgtcgtcg cgccgttgtc gctggtgtac cagacgagcg
     7021 tgttgtcggc gatgtcgagc ttccgcagct cctcgcgcag cttgccaatg gcccggtcca
     7081 ttgccgtgat ctcgccccaa tagttctgca tggccggcgg gtggctggca tagggctcgc
     7141 ggtccgcttc cagcgcctgg tgcggcagat gtggcgagcc gaaccacacg accgccagga
     7201 acggccgctc gctgttggcg gcggcgcgga tgaactcgag cgcggcagcg accgtgacct
     7261 gcgagccttc gccgtcggtc ttgaccacct tgccattggc actcatcagc gggctgttct
     7321 cgtagaagtt cgggctggaa aaccactggt cgaatccgct cgtcccgggc gagttcggca
     7381 cgtcagccgc gaccggcccc aagtgccact ttccgaagtg cccggtcgca taaccagcct
     7441 ctttcaggac ctcggccagc gtgatttctt ccggccgcag catatagccc cacgagaagc
     7501 agccgaagcg attgggatgc cgcccggtga gcacgctgcc gcgggtcggc gagcagaccg
     7561 gcgcggccgc ataaaaccgt tccagccgca gcgccgtccg cgccatctca tccagcaccg
     7621 gcgtcttgag atccgcgttg ccgttatacg ccacatcgcc ccacccctga tcgtcggcca
     7681 tcagcaggac aaagttcggc cgctcggcgg cgcttgtcct ggtggcactc gcgagcaatc
     7741 caactgcgag tccgatggct accaattgga gatacgaacg gagcatggcg gcacctctcg
     7801 caaggattgg aaacaggccc ctcagcataa ccagagcggg ctatgtcttg cgacgcgctt
     7861 tgacgacccg cggcaggccg gcgaggtctt tgaggagcgt ggcgggctcg tagcgggtgt
     7921 cggcggcaat gagatcgacg acgcgggagg cgatcatcgg gctgagctcg acgatcagcc
     7981 agccgccggg aagcagccgc tcggcagcct gcggaatgag ccgttcgata acttccgtgc
     8041 cggctaggcc ggcgacaagc gcttgccgcg gctcgtgatt cctaacctcc ggcgcgagct
     8101 gctcgaactc cgcttccgag acatagggcg gattgctggc gatgaccgca aaccgcggct
     8161 ctggcggcaa ggaggccagc aggtcgcctt ccaggaactc gatccgctcg ctgacgccat
     8221 gctgggccgc attcgccttg gcgatttcca aagcggccgg actggagtca atcgccgtga
     8281 tgtcgactgc aggagcgtgc ttcgcgaccg cgattgcgat cgctccgctg ccggtgccga
     8341 cgtccccgac caaatttgga ccttggacct tggactttgg actatccgcc cggagg
//
LOCUS       DASUGB010000424         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1577302, whole genome shotgun sequence.
ACCESSION   DASUGB010000424 DASUGB010000000
VERSION     DASUGB010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577302"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..702)
                     /locus_tag="VFV87_08225"
     CDS             complement(136..702)
                     /locus_tag="VFV87_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103780.1"
                     /translation="MHDSPPRLRPPQFRLRTLLLLVAAWAAIFAASHWLQPVWVAALV
                     LLALSIVAHVAGNAIGTRLRELGSLKDNRAFDLKPEARGEPRFAPVTRLGLKQSLGWP
                     LVIATAAGIVLGGLGGGLWTLAASRGPIEPAAAALGAIAFAALGGIAAFATVGFTQAL
                     TGAIWQAMRQSAADDKPRHSEIPVRKQF"
     gene            1412..1702
                     /locus_tag="VFV87_08230"
     CDS             1412..1702
                     /locus_tag="VFV87_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103781.1"
                     /translation="MSKQIFSVVSEGAAQATAVVELSPRRAVQVDQREMILSGVVTAE
                     GPVPVAAIIRQVLQRYGLTPEGSEAAAVTPRAETPAHPRTGRECPERAARWD"
     gene            complement(1626..2303)
                     /locus_tag="VFV87_08235"
     CDS             complement(1626..2303)
                     /locus_tag="VFV87_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5103782.1"
                     /translation="MDAATWAFILLALGLIFLVLEFFIPSSGSLAVMCALSLMAAIGV
                     GFAAGPWTGLSILITVAVVVPAALIAAVQWWPETPIGRMILIQRPKSSDEVLPETPAY
                     RTFKDLVGRRGLAKGLMLPSGMVVIDGKSYEAVSEGTPIEAGQPILVVSISTQRLIVR
                     PDNTIRAELAETPSPFADEPADVVAAAKVLAEPSSGESAPANPNEPLVQDIPDPFADE
                     PASPPAG"
     gene            complement(2461..>3786)
                     /locus_tag="VFV87_08240"
     CDS             complement(2461..>3786)
                     /locus_tag="VFV87_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5103783.1"
                     /translation="PVHPKIVNWILRTIEDHQKRNDFNLLILNLRSGGGNVEQSLRLA
                     QQLASLGQPVHTVAYVDWQARGDAAIIALACDELVVAPAAMIGGPGETVISGGELAVI
                     REPLADLARTSGRDWSPSLALLDPSVELHRYTHALGGEVRYLSAEEAQLLPDADQWRR
                     DDVAIETSRGITGLQAEELGLARGTAQNFDELKAMYQIEGTLEQVRPNWVLAAVESLA
                     NPRIAWLLLFVGIFALMFEMSTPGVGIPGLIAVVSFLLFFWSQFLHGTAGWLEILLFV
                     AGLTCLAIEIFALPGFGVFGIGGALMVIVSIVLASQTFVVPANAYQLQQFPVSLLMVA
                     AAMVGGIASIFVIRRFLPDTPYFNRMLLRPPEAEERLALSRREALASWEHLAGKRGVT
                     LTPLVPAGKAQFGDELVDVVSNGEMLPKGTPVVIEEVAGSRVVVRRATG"
BASE COUNT          724 a         1259 c         1178 g          625 t
ORIGIN      
        1 ccgcatcagc gtgcccagcg caaagtcgcc ccgatacacc agccgctcct gcagcgcgat
       61 gcggagaatc gtccaccagg tttgggcgcg agaaaacata cgtggggccg gcatcttgcc
      121 tgccaaaaac agcgttcaaa attgcttgcg gaccggtatt tccgaatgac gtggcttgtc
      181 gtccgccgcc gattgccgca tcgcttgcca gattgccccc gtcagcgcct gcgtaaagcc
      241 gaccgttgca aacgccgcaa tcccgcccag cgcggcaaag gcaattgcac ccaaggcggc
      301 cgccgccggc tcgatcggcc cgcggctggc agccagcgtc cacagccctc cgcccaagcc
      361 gcccagaaca atgcccgccg ccgtcgcaat caccagcggc cagcccaggc tctgcttcag
      421 gcccagacgc gtcaccggcg caaaccgcgg ctcgccgcgg gcttcgggct tcaggtcgaa
      481 ggcccgattg tccttcaagc tgcccagttc ccgcagtcgc gtgccaatcg cattccccgc
      541 cacgtgggcc acaatcgaca gggcgagcaa caccagcgcc gccacccaca ccggctgcag
      601 ccagtggctt gccgcgaaaa tcgccgccca agccgccacc aacagcagca gtgtccgcag
      661 ccgaaactgc ggcgggcgaa gccgaggtgg ggagtcgtgc atggacaaag atgatggttc
      721 gtttgtgaag ttcactgaag cgctgcccaa ccatctcatt gtaggcggca gggcaccatc
      781 attctgcggg catgtggcgc gcaactttgc gggtattttt gagcgcaagt tgcgcgcaac
      841 ctcccgctgc acctccttgc cgctcaacaa cttgcatcga tgccctcccc ccattggtgg
      901 atgttgcgct gttgcgcgtt gctcaaacag ttgcccgctg gcaatttcat gcaaccttac
      961 cttcgctcgc tcacacaccg acacctcggc cccagtctct gcttgggaac acactctctg
     1021 cctgaaaacg cactccgagg cagcgaatct gacattccgt tcacaattgt tcgggagttt
     1081 acatctcgct tccctgcgct ccaagcgaat tccgccgatt ttcctggttc acccggactt
     1141 acagcgaccg ccccgactat aaactcctgt ccaccagcac cttgcgcggt cgctcctcgc
     1201 cctaagtttt ttcgcgtgaa acggaatgga cacgccgcac gggcgaactg cgcgtcggag
     1261 gcgggggcaa gaattccgcg aaaaatattt ttttcgcttg aatggaatgg accagacgtg
     1321 tccatccctc tgcgttgaat ggatgagtgg cctgagcggc ggttggtgcc gcgggggcga
     1381 acattcactc gtttggcaag gagcgcaggg catgtcgaag cagattttct cggtggtttc
     1441 agaaggggcg gcgcaggcga cggcggtggt ggagctgtcg ccgcggcggg cggtgcaagt
     1501 cgatcagcgg gagatgattc tgagcggcgt ggtgacggcc gaggggccgg tgccggtggc
     1561 ggcgatcatc cgccaggtgc tccagaggta tggcctgacg ccagaaggca gcgaggcggc
     1621 agccgtcacc ccgcgggcgg agacgccggc tcatccgcga acgggtcggg aatgtcctga
     1681 acgagcggct cgttgggatt agccggagct gactcgccgg aactcggttc ggcgagaact
     1741 ttagcagcgg cgacaacgtc ggccggttcg tccgcgaacg gcgaaggcgt ctcagccaat
     1801 tcggcccgga tcgtgttatc gggccggacg attagccgct gcgtggagat actgaccacc
     1861 aggatcggct ggccggcttc gatcggcgtc ccctcgctga ccgcttcgta cgacttgccg
     1921 tcgatgacga ccatgccgct ggggagcatc agccccttgg cgaggccgcg gcggccgacc
     1981 aggtccttga acgtgcgata ggcgggggtc tcgggcagga cttcgtcgga cgacttcggc
     2041 cgctggatga gaatcatccg gccgatcggc gtttcgggcc accactggac cgccgcaatc
     2101 agcgccgccg gcacgacgac cgccacggtg atcaggatgg acagccccgt ccacggtccc
     2161 gcggcaaagc ccacgccgat cgccgccatc agcgacaggg cgcacatcac cgccagcgat
     2221 ccgctcgacg gaatgaagaa ctccaggacc agaaagatca gccccagagc gagcaggata
     2281 aaggcccaag tggcggcgtc catacgcgta ttctacggcc gaagcggctg aagccaaaga
     2341 accaagaacc gaagaaacaa gaaccaaggg cgggagcgga agatcgaaga aacaagaacc
     2401 gaagatccaa ggaagctcgc tcggccttgg ttcttcggtt cttgtctctt ggttcttacg
     2461 ctaccccgtt gctcgccgga cgacgacgcg actgccggcg acctcctcga tgacgacggg
     2521 cgtgcccttg ggcagcatct cgccattgct gacgacatcg accagctcat cgccgaactg
     2581 agccttgccc gccggcacca gcggcgtgag cgtcacgccg cgcttgccgg ccaggtgctc
     2641 ccagcttgcc agggcctcgc ggcggctgag ggccaggcgc tcctcggcct ccggcgggcg
     2701 gagcagcatg cggttgaaat acggcgtatc gggcaggaac cggcggatca caaagatcga
     2761 cgcgatcccc cccaccatcg ccgccgccac catcaggagc gaaacgggga actgctgcag
     2821 ttgataggca ttcgccggca cgacgaacgt ctgactcgcg agcacgatcg acacgatcac
     2881 catcagcgcc ccgccgatgc cgaacacgcc gaagcccggc agcgcgaaga tttcgatcgc
     2941 caggcaggtg agaccagcga cgaacagcag aatttccagc cagcccgccg ttccgtgcag
     3001 aaactgcgac cagaagaaga gcaggaacga aacgacggcg atcaggcccg ggatgccgac
     3061 gcccggcgtg gacatctcga acatcagggc gaagatgccg acgaacaaca gcagccaggc
     3121 aatccgcgga ttggccaggc tttcgaccgc cgccagtacc cagttcggcc ggacctgctc
     3181 cagcgtccct tcgatctgat acatggcctt cagctcgtcg aagttctgag ccgtgccgcg
     3241 ggccaggccc agctcttcgg cttgcaggcc ggtgatgccg cggctggttt cgatcgccac
     3301 gtcgtcgcgc cgccactggt cggcatcggg cagcaattga gcctcctcgg ctgagagata
     3361 ccgcacttcc ccgccgagcg cgtgcgtata gcggtgcaat tcgacgctcg gatcgagcag
     3421 agcgaggctt ggcgaccagt cgcggccgct cgtgcgggcc aggtcggcca gcggctcgcg
     3481 gatgacggcc agttcgccac cgctgatgac cgtctcgcct ggcccgccga tcatcgctgc
     3541 cggtgccacg accagttcat cgcaggccag ggcaatgatc gccgcgtcgc cgcgggcctg
     3601 ccaatcgaca taggccaccg tgtgaacggg ctggcccagc gaagcaagct gctgcgccag
     3661 ccgcaggctt tgctcgacgt tccccccgcc gcttcggagg ttgaggatca gcaggttgaa
     3721 atcgttgcgt ttttggtggt cctcgatcgt ccgcaggatc cagttgacga tttttggatg
     3781 caccgg
//
LOCUS       DASUGB010000425         3883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1298530, whole genome shotgun sequence.
ACCESSION   DASUGB010000425 DASUGB010000000
VERSION     DASUGB010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298530"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFV87_08245"
     CDS             <1..1000
                     /locus_tag="VFV87_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX5103784.1"
                     /translation="FDASLEDIAGRLDREAQTAHDDEEGLEDDRLRLIFTCCHPAIAP
                     EAQVALTLREVCGLTTEEIARAFLTAPPTLAQRIVRAKAKIKSAKIPYQVPSRGDLPE
                     RLDAVLRVVYLVFNEGYSASSGQSLTRADLSGEAIRLGRLLMELLPEPEVVGLLALML
                     LQESRREARSTASGQIVLLEDQDRTLWNREQIAEGLELVGQAVASQQFGSFTLQAAIA
                     SVHAQAPAAAETDWNQIVSIYDVLLQAEPSPIVELNRAVAVAMRDGPEAGIALIDGIL
                     TRGELAEYHLAHAARADLCRRAGQRAAARQCYERALALARLEPERQFLQRRLDEMS"
     gene            1144..1482
                     /locus_tag="VFV87_08250"
     CDS             1144..1482
                     /locus_tag="VFV87_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103785.1"
                     /translation="MRFQGLFTSAQPAAVDRASRLSLLEKVSRLMDEAANTAPSKSLN
                     GLDAMSARVKLAAGEFTITAAPASEPTARGASYAMFDVESIVEAILWTKCFLQVLGEG
                     ECEIRPLCAA"
     gene            1569..2447
                     /locus_tag="VFV87_08255"
     CDS             1569..2447
                     /locus_tag="VFV87_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5103786.1"
                     /translation="MRFMVLVKANSDSEAGKMPSTELLTEMGKFNEELVKAGVMLAGE
                     GLHPSSKGVRARYANGKVTLTDGPFSETKELVAGYWLWQCKSKDEAIEWLKRSPFQEG
                     EVEIRPLFELEDFPVDPAEQPGGWREKEEQFKAAPPPARRPGTKRYLGIVKADKDTEA
                     GVMPDEKLLAAMGAFMEEGIQKGIFLSGEGLQPSSKGARVRFSGSQRTVIDGPFTETK
                     ELAAGYAVLQFASKAEAVEWTKRFVQVDAPGRLNGESVCELRPIFDAEDFGEALTPEL
                     KAQEDRLRTDIESQRK"
     gene            2664..3026
                     /locus_tag="VFV87_08260"
     CDS             2664..3026
                     /locus_tag="VFV87_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5103787.1"
                     /translation="MRYLCLGYHDEHAWNSLPEAERQQLLADTQAYEHDLRGGGHVVD
                     TKGLQPAASAATLRFGGGRVTVTDGPFAETKEQLGGVMVLEAKDLNHAIQLMSQMPCM
                     RIGGSLEIRPLNEELSQP"
     gene            3023..3484
                     /locus_tag="VFV87_08265"
     CDS             3023..3484
                     /locus_tag="VFV87_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX5103788.1"
                     /translation="MIEPPQIVNSPAQHIACIRLKIPPAEIRQVMMPGLQEIMAALAA
                     QGMKPAGPWLTHHFQKPVEMFDFEIAVPVATPIAPSGRVQPGQLRATKVARTVYHGGY
                     EGLVAAWGEFCDWISAEGLQTAPDLWECYAKGPESGEDSSNWETQLNQPLA"
     gene            3560..>3883
                     /locus_tag="VFV87_08270"
     CDS             3560..>3883
                     /locus_tag="VFV87_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5103789.1"
                     /translation="MKYMLLIYMNEGAMNQAEREACFGESTRLCHDLAAQGKFITASP
                     LHPVARATSVRIRNGQRLVTSGPFAETHEQLGGFYLIDAKDLDDAIAIAARIPPAAKG
                     PVEIRP"
BASE COUNT          770 a         1248 c         1186 g          679 t
ORIGIN      
        1 gttcgatgcc tcgctggaag acatcgccgg ccggctggac cgcgaggccc agaccgctca
       61 tgacgatgaa gaggggctcg aagacgaccg actgcggctg attttcacgt gttgccatcc
      121 ggccatcgcg ccggaggccc aggttgccct cactcttcgc gaagtttgtg ggctgacaac
      181 cgaggaaatc gcgcgggcct ttctcaccgc gccgccgacg ctcgcccagc gaattgtgcg
      241 ggccaaggcg aaaatcaaaa gcgccaaaat cccttaccag gttccgtcgc gcggcgatct
      301 tcccgagcgc ctggatgccg ttctccgagt ggtctatctc gtgttcaacg agggttattc
      361 cgcatcttcg ggccagtcac ttacacgtgc ggatttatcc ggcgaagcga tccgcctcgg
      421 ccggctgctg atggaattgc tcccggaacc ggaagtcgtc gggctgctcg cgctaatgct
      481 cctgcaggaa tcgcggcgcg aagcccgcag caccgcgagc ggccagatcg tactgctcga
      541 agatcaggac cggacacttt ggaaccgcga acaaatcgcc gaggggctgg aactcgttgg
      601 gcaggccgtt gcgtcgcagc agttcggctc gttcacgttg caagccgcga ttgcctccgt
      661 tcacgcgcaa gccccggccg ccgccgaaac cgactggaac caaatcgtca gtatttacga
      721 cgtgctttta caggccgaac cctcgccgat cgtcgagctg aatcgtgccg tcgcggtggc
      781 aatgcgcgac ggccctgagg ccggaattgc gttgattgac ggaattctca ctcgcggcga
      841 actggccgag tatcacctcg cccacgccgc tcgcgccgat ctctgccgcc gcgccggcca
      901 gcgagccgca gcccggcagt gctacgaacg ggccctggcc ctcgcccgcc tggagccgga
      961 gcgccagttc ctccagcgcc ggctggacga gatgtcgtag tcgcctccgc agcgaatgtc
     1021 gctgtttcgg atttttttgt gccagctgtc gaatcgggag tcgctcaatc gactatccag
     1081 tgaaagacga agtcgacgcc aagagggcga ctctaactcc taagcgtaca agggtttata
     1141 gccatgcgat tccagggact attcacctcc gcccagccag ccgccgtgga ccgcgcgtcg
     1201 cgcttgagcc tgctcgaaaa ggtcagccgg ctgatggacg aagcggccaa caccgcaccc
     1261 tccaagtcgc tgaacggcct ggatgcgatg tccgcccgcg tcaagttggc ggcgggcgaa
     1321 ttcaccatca cggccgcgcc ggcatccgaa ccgacagccc gtggcgcctc ctacgccatg
     1381 ttcgacgtgg agtcgatcgt ggaggcgatt ttatggacca agtgctttct gcaggtcctc
     1441 ggcgaaggcg agtgcgagat tcgtccgctc tgcgcggctt aggaacgccc ccgattggct
     1501 cacaaactgc acactctgtt cacttccctc tcaagctcac tcaacgacat tcgtctggag
     1561 gttccaacat gcgtttcatg gttctcgtaa aagcgaattc cgattccgaa gccggcaaaa
     1621 tgcccagcac ggaattgctc accgaaatgg gcaagttcaa cgaagagctg gtcaaagccg
     1681 gcgtgatgct cgccggcgag ggattgcacc ccagctccaa gggcgtgcgt gcccgttatg
     1741 ccaacggcaa ggtcacgttg accgacgggc cgttttccga gacgaaggag ctggtggccg
     1801 gctactggct atggcagtgc aaatcgaaag acgaggcgat cgagtggctc aagcggtcgc
     1861 cgttccagga aggcgaggtc gagattcgcc cgctcttcga gctggaagac tttccggtgg
     1921 accccgccga acagcccggc ggctggcgcg aaaaggaaga gcagttcaaa gccgccccgc
     1981 ctcccgctcg gcggcccggc acgaagcgat acctgggcat tgtcaaagcg gacaaggaca
     2041 ccgaggcggg agtcatgccc gatgagaagc tgctggcggc gatgggggcg ttcatggagg
     2101 aagggatcca gaagggcatt ttcctttcgg gtgaagggct gcagcccagc tccaaagggg
     2161 cacgggtccg ctttagcggc agccagcgaa cggtcatcga cgggccattt accgaaacga
     2221 aagagctggc cgctggctac gccgtgttgc agttcgcctc caaagccgag gcggtcgagt
     2281 ggacgaagcg gttcgtccag gtggacgcgc ccggccgcct gaacggcgag agcgtctgcg
     2341 agctccgtcc gatcttcgat gcggaagatt tcggcgaggc cctcacgcct gagctcaagg
     2401 cccaggagga ccgcctccgc accgacatcg aaagccagcg aaaataatct cagaaaaatt
     2461 tcgatcgcct gtcgattcgg cgtccgccca ttcgaccact ggttgccgga tacgactcac
     2521 cactcactac tgcttcgtca atgcttggac gacaggtacg tgccgcgtag cggcggcatc
     2581 ttgccgccgc atttcggcgg ctggaagccg ccgctacgaa tctcaggctt gacaaagcac
     2641 cgctcaccac caacctgctc cccatgcgtt acctctgcct gggataccac gacgaacacg
     2701 cctggaattc cctgccagaa gccgaacggc aacagttatt ggccgacacc caggcctatg
     2761 aacacgatct ccgcggcggc ggccacgtgg tggataccaa gggcctccag ccggccgcga
     2821 gtgccgccac gctgcgcttc ggcggcggcc gggtcaccgt cacggatggc ccgtttgctg
     2881 aaacgaagga gcagctcggc ggcgttatgg tcctcgaagc caaggacttg aatcacgcca
     2941 ttcagctcat gtcccagatg ccctgcatgc gcatcggcgg ctcgcttgaa atccggccct
     3001 tgaatgagga actctcgcaa ccatgattga acctccccag atcgtcaatt cccctgctca
     3061 acacatcgcc tgcatccgcc tcaagatccc gccggcagaa atcaggcagg tcatgatgcc
     3121 tgggctgcag gaaatcatgg cggctcttgc cgcgcaggga atgaagccgg ccggaccctg
     3181 gctgacgcat catttccaga agcccgtcga gatgtttgac ttcgagattg ccgtgccggt
     3241 ggcgactccc atcgcgccga gcggccgcgt gcagcctggg cagttgcggg cgacgaaagt
     3301 tgcccgtacc gtttatcacg ggggctatga aggtctggtc gcggcctggg gcgaattctg
     3361 cgactggatc tctgcggagg gactccagac cgcgcccgat ctgtgggagt gctacgccaa
     3421 gggtcccgaa tccggcgaag actcctcgaa ctgggaaaca caactcaacc aaccactcgc
     3481 gtagccgcgg cttccagccg ccgcgggcgc agtttcacaa ttgcggcggc tagaagccgc
     3541 tgctacggag agtcaaacca tgaaatacat gctgctgatc tacatgaacg aaggggcgat
     3601 gaaccaggcc gagcgcgagg cgtgcttcgg cgaatcgacc cggctgtgcc acgacctggc
     3661 cgcgcagggc aagttcatca cggccagtcc gctgcacccg gtcgcaaggg cgaccagcgt
     3721 ccgcatccgc aatggccagc ggctggtgac cagcggaccc tttgccgaaa cccacgagca
     3781 acttggcggc ttctacttga tcgacgccaa agatttggat gacgcgatcg cgatcgccgc
     3841 cagaattccg ccggctgcca aggggccggt cgaaatccga ccc
//
LOCUS       DASUGB010000426         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_743147, whole genome shotgun sequence.
ACCESSION   DASUGB010000426 DASUGB010000000
VERSION     DASUGB010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743147"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3067
                     /locus_tag="VFV87_08275"
     CDS             <1..>3067
                     /locus_tag="VFV87_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5103790.1"
                     /translation="SIETVARERGLNAKYLGTLWNSLNAKQPSLLLDGLRARWRSAKP
                     ADAAALAAEIGAWQKGLWRFATVGHIGKAGGPKAWMEPVSPLVAGQELRLKIPTPAEG
                     EDVTISLVAADAGDGNEHDYVVWQQPRLVGPGRRDLLLRDVRQVTRELTARRDRLFAS
                     AAKYLAAADQAAAARGEAKPEALAKQHGLEARDLRAWLDYLGIGSGGPVELTGHFMEK
                     LTSAAGYDFIQGWGSHNTPLLLANSSDQHVRVPGNMQPHGVAVHPSPTLRAAVGWQSP
                     ITAKMQIEGQVTHAHPECGNGVTWSLELRRGATRQRLAAGIAHGGKPVPIGPLADVAV
                     QKGDLVSLLIGPRDGNHACDLTAVELKLRCNDDAEKAWSLAADVSGDVLAGNPHNDRQ
                     GNAGIWHFYTEPDKGGEVGPVIPPGSLVAKWLAAKNSDDRQQLAGQVQKLLTAGPPAD
                     KQSPDAVLYRQLASLGGPLLAGVLSQPLAVQDNAAPLVEEDFGLDPTRFGKHPDGREI
                     DAASLCVRAPAAISFRLPSDLAAGCELVTTAVLHPETGREGSVQLDLALGKATADSGL
                     LPSEAKTTRGGGQWTADNRRTAYSAPILVNEGSAARQRIERDLGEFRQLFPAALCYTK
                     IVPVDEVVTLTLFYREDEHLVRLMLDDAQRQEIDRLWDELHYISQDSLTLVDALEQLI
                     QYATQDADPSVFTPLREPFAARAAAYRQRLIDDEPRQVDALIAFAARAYRRPLAAGEE
                     QELRGLYAKLRQQELPHEEAFRLTLARIFVSPSFLYRLENAPPSEQPGPVSDWELASR
                     LSYFLWSSQPDDELRALAAAGKLQDAGVLAAQARRMLQDPRTRRLASEFACQWLHIYD
                     FAALDEKSERHFPDFAQLRGDMQEESIRFFADLFQNGGSVLDLLDADHTFVNAALAKH
                     YGIEGVAGNDWRRIENAKQYGRGGILALSTTLAKQSGASRTSPILRGNWVSEVLLGEK
                     LPKPPKDVPLLPDEAATGELSVRELVQRHTSDERCANCHARIDPFGFALE"
BASE COUNT          515 a         1092 c          989 g          471 t
ORIGIN      
        1 gtcgatcgaa acagttgccc gcgagcgcgg gctgaacgcg aagtatctcg gcacgctctg
       61 gaacagcctc aatgcgaagc agccgtcgct gttgctcgat ggcctccgcg ctcgctggcg
      121 gtccgcgaag cccgccgatg ccgcggcgct ggcggcggaa atcggcgcgt ggcaaaaggg
      181 gctctggcgg ttcgccaccg tcggccacat cggcaaggcc ggcgggccga aagcgtggat
      241 ggaacccgtc agcccgctcg ttgccggcca ggaactgcgg ctgaagattc cgacgccggc
      301 tgaaggcgag gacgtgacga tttcgctcgt cgccgccgat gcgggcgacg gcaacgagca
      361 cgactacgtc gtctggcagc agccgcgatt ggtcggcccg ggccggcgcg acttgctcct
      421 ccgcgacgtg cggcaagtga cgcgcgagct gaccgcccgc cgcgatcgac tgtttgccag
      481 cgccgcgaag tacctcgccg ccgccgatca ggctgccgcc gcacggggcg aggccaagcc
      541 ggaagcgctg gcgaagcaac acggcctgga ggcccgcgac ctgcgagcct ggctcgacta
      601 tctcggcatc ggctccggcg ggccggtgga gctgaccggc catttcatgg agaagctcac
      661 cagcgccgcc ggctacgact tcatccaggg ctggggtagc cacaatacgc cgctcttgct
      721 cgcgaattcc tccgaccagc acgtccgcgt ccccggcaac atgcagccgc acggtgtggc
      781 cgtgcatccc tcgccgacgc tccgcgccgc cgtcggctgg cagagcccga tcacggcgaa
      841 gatgcagatc gagggccagg tgacgcacgc ccatcccgag tgcggcaacg gcgtaacctg
      901 gtcgctggaa ctccgccgcg gcgcgacgcg ccagcggctg gcggcgggaa ttgcccacgg
      961 cggcaaaccg gtccccatcg gcccgctggc ggatgtcgcg gtgcagaaag gcgacctggt
     1021 ctcgctgctc atcggtcccc gcgacggcaa tcacgcctgc gacctgaccg ccgtcgagct
     1081 gaagctgagg tgcaacgacg acgccgagaa agcttggtcg ctggccgccg atgtatcggg
     1141 cgatgttctg gccggcaatc cgcacaacga tcgccagggc aacgcgggca tctggcactt
     1201 ctacacggaa ccggacaaag gcggcgaagt cgggccagtg attccgccgg gatcgctcgt
     1261 ggcaaagtgg ctcgcggcta aaaactccga cgaccgccag caactggccg gacaagtgca
     1321 gaagctgctc accgctggcc cgcctgcgga caaacaatcg cccgacgccg tcctctatcg
     1381 tcagcttgcc tccctcggcg gtccgctctt ggccggcgtg ctttcccagc cgctcgccgt
     1441 ccaagacaat gccgcgccgc tcgtggaaga ggacttcggc ctcgatccca cccgcttcgg
     1501 caagcatccc gatggccgcg agattgacgc cgccagcctg tgcgtccgcg ctccggctgc
     1561 gatcagcttc cgcctgccgt ctgatctggc ggcgggctgc gagctggtta ccaccgccgt
     1621 cctgcacccc gaaacggggc gcgaagggag cgtgcagctc gatctggccc tcggcaaagc
     1681 gacggccgat tcgggcctgc tcccgagcga agccaagacc acccgcggcg gcggccagtg
     1741 gaccgccgac aaccgccgca cggcttattc ggcgccgatc ctcgtcaacg aaggcagtgc
     1801 cgcccgccag cggatcgagc gagacctggg cgaattccgc cagctctttc ccgccgccct
     1861 ctgctacacg aagatcgtcc ccgtcgatga agtcgttacg ctcaccctct tttaccgcga
     1921 ggacgagcac ctcgtgcggc tgatgctgga cgatgcccag cggcaagaga tcgaccgctt
     1981 gtgggacgag ctgcattaca tcagccagga ttcgctcacg ctggtcgatg ccctggagca
     2041 gctcattcag tatgccacgc aggatgccga cccctcggtc ttcacgccgc tccgcgagcc
     2101 cttcgccgca cgggccgctg cctatcgcca gcggctcatc gacgacgagc cgcggcaagt
     2161 tgatgcgctt atcgccttcg ccgcccgcgc gtaccgccgg ccgctcgccg cgggcgaaga
     2221 gcaggagctg cgcggcctgt atgcaaagct ccgccagcag gagctgccgc acgaagaggc
     2281 gttccgcttg acgctcgccc gcatcttcgt ctcgccgtcg tttctctacc ggctggaaaa
     2341 tgctccgcca agcgagcagc cgggaccggt ctcggattgg gagctcgcct ctcgtttgag
     2401 ctatttcctc tggtcgtcgc agccggatga cgagcttcgc gccctggcag cagcgggcaa
     2461 gctgcaagac gcaggagtcc tcgccgctca ggcgcggcga atgctccagg acccgcgcac
     2521 ccgccgcctg gcgagcgagt tcgcttgcca gtggctgcac atttacgact ttgccgccct
     2581 cgacgaaaag agcgagcggc actttcccga tttcgcgcag cttcgcggcg acatgcagga
     2641 ggaatcgatc cgcttctttg cggacctgtt ccaaaacggc ggctccgttc tcgatctgct
     2701 cgacgccgat cacacgtttg tgaatgcagc gctggcgaag cactatggca tcgaaggcgt
     2761 cgcgggaaac gactggcggc ggattgaaaa cgcgaaacag tacggccgcg gcggcatcct
     2821 ggcgctttcc accacgctgg ccaagcaatc gggcgcctcg cgcaccagcc cgatcctccg
     2881 cggcaactgg gtctccgaag tgctgctggg cgagaagctc cccaagccgc ccaaggacgt
     2941 gccgctgctt cccgacgaag cggcgaccgg cgagctctcc gtccgcgagt tggtccagcg
     3001 gcacacgagc gacgaacggt gcgccaattg ccacgcccgc atcgacccct tcggcttcgc
     3061 cctggag
//
LOCUS       DASUGB010000427        10396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1436495, whole genome shotgun sequence.
ACCESSION   DASUGB010000427 DASUGB010000000
VERSION     DASUGB010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436495"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFV87_08280"
     CDS             <1..790
                     /locus_tag="VFV87_08280"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5103791.1"
                     /translation="TGGDYYDFFSLLDGGLCIAIGDASGHGISAALIMEETRASLRAL
                     ALTGADAGAIVALTNRCLVNDLPTGQFVTMFLARLDPGTRSLVYCSAGHPSGYVLDQH
                     GKVRLVLQSTNIPLGLDLTAEFLTTPAITLLTGDLLLLYTDGVTESFSPEDSLFGIER
                     MLDVVRAHRHKSPAEIVQALFGAVADYSGNHAQLDDITAVIIKVEASTLLGLAPPQSA
                     VKNHEPDRDLFCRAARSRVRTVPKLERQSRLSRWNKYVTEAFPH"
     gene            1136..9925
                     /locus_tag="VFV87_08285"
     CDS             1136..9925
                     /locus_tag="VFV87_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX5103792.1"
                     /translation="MRVPDISDKLLRLMSWRRSTKGESPLTRHADELPLRAELFSIDQ
                     LERHARTIAASHRLATGRARDKLLSRLDDNERVLIETYDLVTAAADQNRRIEPAAEWL
                     LDNFYLIEEQIRAVRRLLPPSYSRELPRLVSGPAASFPRAYGIALELIAHVDGRVDGA
                     SLNGFITSYQSVEPLKLGELWAVPLMLRLALIENLRRVAVRIAAARRDRDLASDWAER
                     MVSVVERKPTDLILVLADMARANPPLSGAFLAELTRHLQGHNPNFAFANSWLEHRLAD
                     QGLTTEQLVRAEGQAQAADQVSIGNSINSLRFVNAYDWRQFVGEHSLVEQTLAEDPAR
                     FYTQMDFATRDQYRHAVEGIARRSRFTEYDVARKAVQLAETQARETPTGRAAHVGYFL
                     VDRGRPVLERLAEMRLSAAVVLDKARRRFPLTCYLTPMTLVTLAATFLFLWWTLRSGE
                     EWLAAALLTVPVLISASQLGLALVNWLATHVLSPQSLPRMNFEQGIPSEHRTLVAVPT
                     MLTSAEGIEHLLDGLEVRYLANRDANLHFALLTDFADAPAETMPADEELVRLAREGIE
                     QLNEKYAGADGNIFYLLHRARLWNEQEGVWMGYERKRGKLADLNALLRGAKDRFAEVV
                     GQTAILQRVRYVITLDTDTQLPRDAARQMVGTIAHPLNRPVFADHCRRVVDGYTILQP
                     RVGVSLPSAQRSRFVQLFAGDPGIDPYTRVVSDVYQDMFGEGSFIGKGIYDVDSFECC
                     CGDFPENAILSHDLIECAYARSALLSDVVLYEEHPARYAGDVARRHRWMRGDWQIAPW
                     ILPRVPGRSGKRVRNPISALSQWKIFDNLRRCLVPVAMVLLLVISWLLSPAMATAALV
                     FVATVVALPPLLTAAADLLRKPADLPLGMHSRATLHMLARSLAQGVLLCVFLPYEAFV
                     SADAVVRTLVRMTWTKRRLLEWKTASDSERGADGSLEETYRTMAFAPALGASALLLLV
                     FYDRQVLLHAGPWIAAWMASPFIAWWLSQPFPRQELQFSDNQRHFLEKLSRKTWRYFE
                     DFVTAEDNWLPPDNVQQNPGLAISPRTSPTNIGMALLADLAAYDFGYCSAARLLARTK
                     RTLETMLLLERYRGHFLNWYQTRTLAPLHPQYVSTVDSGNLAASLLVLGSGCLELSET
                     RILPPRWSGGLRDTLRVLLDAAHGTAGPPVSADVLRKMERQIDELEQAPATLAAAGAL
                     LSQLSVAAAELTAVAGSDAEVSWWAGAYELSCTDHRTDLNHLAAWLALPAAPAGTWEC
                     DCPKQREHLSKLRQMLDGLDRAATLPDVAGLQQTALPLIDAALPTAAAAAKQWLAQLR
                     TALADSAEHAAARIHALKEVAEKCRELADMDFSLLYDPGRELFSIGYNLSERRLDASF
                     YDLLASEARLASFVVIAQGHFGQEHWFALGRLLTSAGSVPTLLSWSGSMFEYLMPLLM
                     MPTYENTLLDRTYHAAVRRQIEYGRQRGVPWGISESGYNTFDQHKTYQYRAFGVPGLG
                     LKRGLVEDLVIAPYASALALMVAPEAACRNLERLTADGHQGPYGFYEAVDYTPSRLPP
                     GVERVTVRQFMAHHQGMSLLSLAYVLLERPMQRRFLADPMLRAAILLLQERVPTAVAP
                     VYPHATEASATRLASGEETGSMRVFTDPSSATVEAHLLSNGRYHVAVTNAGGGYSRWC
                     DVAVTRWREDATRDSYGSFSYLRDIDSGAVWSSTHHPTTTRTKGYEAIFTQSRAEFRR
                     TDEEIETYTQICVSPEDDIELRRITLTNRGELPRTLEITSYAEVVLAAQAQDEAHAAF
                     SNLFVQTELVPGCQAIYATRRPRSAEERPPWMTHMMAVRGVTIGEPSYETDRMRFIGR
                     HRTLASPAALDRATPLSNTAGPVLDPIVSIRQVVLIMPNESVRIDIITGVAESRAAVD
                     GLTEKYRDASLADRVFDLAWTHSHILLQQLSASEAEAQVYGRLAGSIIYASALRRAKA
                     SILAHNRRGQSGLWGYGISGDLPIVLVRIRDRQRIGLVQQAVQAHAYWRLKGLVVDLV
                     IWNEDDSVYRQELQQAIVDLVAASPEASLVDRPGGIFIRRGEQMSDEDQALMQTVARI
                     VLVDEAGTLAEQAERRGRSDVSIPPLKPVRRQSEPVSYEPPQRDLAFFNGLGGFSHDG
                     REYVTILAPDQTTPAPWVNVIANAQFGTVVSEGGSAYTWAENSREFRLTPWRNDPVSD
                     ISGEAIYLRDEESGRFWSPSPLPARGKNTYMVRHGFGYTIFDYTEEGLTTELCVYVAT
                     DAPVKFYKLKIANRSGRTRQLSVTGYWELVLGDFRSKTLLHVVTESDPTSGAILARNV
                     YSPEFGDRVAFFNCSEMARTFTGDRAEFLGRNGQFASPSAMRRVRLSGRVGAGYDPCA
                     AFQAPLTLADGQERIITFTIGAAGGAAEARSLAQRYRSVDSAHRAIEGVWDYWSRTLG
                     VVYLETPDPRVNYLANGWLIYQTLACRMWARTGFYQSGGAYGFRDQLQDAMALVHAQP
                     TLLREHLLRAASRQFREGDVQHWWHPPLGRGVRTHFSDDFLWLPLAICRYVGITGDTG
                     VLDERIPFLTARLLRDDEESNYDLPQISHEIGTLYEHAVRAIDHGLRFGQHGLPLMGS
                     GDWNDGMNLVGHRGKGESVWLAFFLFQVLTQFAKLARRRGDVTLADRYTVEAGRLRGN
                     IEENGWDGQWYRRAYFDDGTPLGSAANEECQIDAISQSWSVLSGAGTPERRIAALSSV
                     DKRLVRRDARLILLLDPPFDKSNLDPGYIKGYVPGVRENGGQYTHSAIWTVMAMAAMG
                     DRERAWELFSLINPISHGATADDIATYRAEPYVVAADVYGVDPHTGRGGWTWYTGSAG
                     WMYRLIVESLLGLHLEVDKLRFAPCLPAQWTEFKIHYRYRETFYHITIRNDAAGGTVG
                     RLVLDGAELSDDFVTLIDDRQDHELEVELRPDDISTQPILGGD"
     gene            10087..10377
                     /locus_tag="VFV87_08290"
     CDS             10087..10377
                     /locus_tag="VFV87_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103793.1"
                     /translation="MGIKPKRNMETGEDETWELKDLRKTCATFYDEHVPESSIEILGH
                     SVSGVTYRHYAHRAPLAFKAIMTLPQPSAFTGLVRGFDGQCPCCRRPFADRS"
BASE COUNT         1818 a         3343 c         3344 g         1891 t
ORIGIN      
        1 aacaggcggg gactactacg actttttttc cttgctcgac ggcggcctct gcattgccat
       61 cggcgatgcc agcggccacg gcatctccgc cgctctcatc atggaggaga cccgcgcgtc
      121 gctgcgagcg ctggccttga ccggggccga tgccggtgcg atcgtcgcgc tcaccaaccg
      181 ttgcctcgtg aacgaccttc ccacgggtca attcgtgacc atgtttctgg cgcggctcga
      241 ccctggtacg cgctccctcg tttactgtag cgccggtcac ccttccggtt acgtcctcga
      301 ccaacatgga aaggtgcgtt tagtccttca aagtaccaac atcccactgg gcctcgattt
      361 gactgccgag tttctcacca cgccggccat aacgctgctg acaggcgacc tcttgttgtt
      421 atacaccgat ggggtcacgg aaagtttttc accagaggac tcgctttttg gcattgaaag
      481 gatgctggat gtcgtccggg cgcaccgcca caagagcccc gctgagattg tgcaggcgct
      541 gtttggtgcc gtggctgatt attctggaaa ccacgctcaa ctcgacgaca tcacggccgt
      601 cattatcaaa gtcgaagcca gcactttact cggtcttgca cctcctcaat cggcggtcaa
      661 gaaccatgag ccggatcggg acttgttttg tcgagcggca agatcgcggg tccgcactgt
      721 gccaaaacta gaacggcaat caagactcag ccgctggaac aagtacgtaa cggaggcgtt
      781 cccgcattga cgcaaatcca gtcggcgcac tctgcggacg cagtttttta gtcaccagca
      841 ggcgcgcttc ggtgaggcac cccggcgaac tgaacccggg cgatgggacc gaagcgtcca
      901 agagtgcggt ccaaatcgcc ggccctgacg cgaaaagaag gatgttcgat ggtgagagac
      961 gccagattgc gagatagggg ccagcgatgc gaacgaacga tctagcgcta cgccggcaaa
     1021 ctatacggcc agcgctttca cgatagcggt ggcttcgtgt catggcagac gaggactata
     1081 ctcaagtctt tcgctcttga gttcgcgaat tgctcaagat gtacaggagg gttaagtgcg
     1141 tgtcccggac atctcagaca agctgctgcg gctgatgtcc tggcgacgtt ccacgaaggg
     1201 ggaatccccg ctcaccagac acgcggacga actgccgcta cgagcagaac tcttcagtat
     1261 cgatcagctc gaacggcacg cccggacgat tgccgcgtcc caccgcctgg ccacgggccg
     1321 ggcccgcgac aagctgctct cgcggctgga cgacaacgag cgggtgctga tcgagaccta
     1381 cgatctggtg acggcagctg ccgatcagaa ccgccgcatc gagccggccg ccgaatggct
     1441 cctcgacaac ttctacctga tcgaggagca gatccgcgcc gttcggcgct tgctcccccc
     1501 gtcgtacagc cgcgagctcc cacgccttgt cagcggaccg gccgccagct ttccccgcgc
     1561 gtacggcatc gccctggaac tgattgccca cgtcgacggg cgggtcgatg gggccagcct
     1621 caacggcttc atcacctcat accagtcagt ggagccgctc aagctgggag aattgtgggc
     1681 ggtgccgctg atgctgcggc tggcgctgat cgagaatttg cggcgcgtcg cggtgcggat
     1741 tgcggccgcg cgccgcgacc gcgatctggc cagcgactgg gcggagcgga tggtcagcgt
     1801 ggtggagcgc aagcccaccg acttgattct ggtgctggcg gacatggccc gcgccaatcc
     1861 gcccctgtcg ggtgcattcc tggccgagct gacgcgccat ctgcaggggc ataatccgaa
     1921 tttcgctttc gccaatagct ggctcgagca tcgcctggcc gatcaaggct tgacgaccga
     1981 gcagctcgtg cgcgccgagg gacaggccca ggcggccgac caagtgtcga tcggcaacag
     2041 catcaacagt ctgcggtttg tgaacgcgta tgactggcgg cagttcgttg gcgagcacag
     2101 cctggtcgag cagaccctcg cggaagatcc ggcgcgattc tatacccaga tggatttcgc
     2161 gacccgcgac cagtatcggc acgcggtcga agggattgcc cgccgcagcc ggtttaccga
     2221 atacgacgtg gcccgcaagg cggtgcaact ggccgaaacg caagctcggg aaacacccac
     2281 cggccgcgca gcgcacgtgg gatacttcct ggtcgatcgc ggccggccgg tgctggagcg
     2341 gctggccgaa atgcggctct cggcagcagt ggtcctcgac aaggctcggc gaagatttcc
     2401 gttgacgtgt tacctgactc cgatgacgct tgtgacccta gcggccacgt ttctgttcct
     2461 gtggtggacg ctgcggagcg gcgaggagtg gctggccgcg gcgctgctga ccgtgccggt
     2521 gttgatcagc gcctcccagc ttgggctggc gctcgtcaac tggctggcga cccacgtcct
     2581 gagcccgcag tcgctgccgc ggatgaattt tgagcagggc attccgtccg aacatcgcac
     2641 gctggtcgcg gtccccacaa tgctcacgag tgccgaggga atcgagcatt tgctcgacgg
     2701 gctggaggtg cggtacctgg ccaaccgcga cgcgaatctg cactttgcct tgctgactga
     2761 ttttgccgat gcgccggcgg aaacgatgcc cgcagatgag gagctggtgc ggctggcgcg
     2821 ggaagggatt gagcagctca acgaaaaata cgccggcgcc gacggcaata tcttttatct
     2881 cttgcaccgg gcgcgcctgt ggaacgaaca agaaggcgtg tggatgggct acgagcggaa
     2941 gcgcggcaag ctggccgatc tgaacgccct gctccgcggc gccaaagacc gctttgcgga
     3001 agtcgtcggc cagaccgcca tcctgcaacg cgtgcgttac gtcatcaccc tcgataccga
     3061 cacgcaactc ccccgcgatg cggcgcggca gatggtcggg accatcgcgc atccgctcaa
     3121 ccggccggtt ttcgcggatc actgccgacg cgtcgtggac ggctatacga tcctgcagcc
     3181 gcgggtgggc gtcagcctcc ccagcgcgca gcggtcgcgc ttcgtacagt tgttcgcggg
     3241 agatcctggc atcgatccct atacgcgcgt cgtgtccgac gtgtaccagg acatgttcgg
     3301 cgagggctcg ttcatcggca aagggattta cgacgtcgat tccttcgagt gctgctgcgg
     3361 cgactttccc gagaacgcca tcctgagcca cgatttgatc gagtgcgcct atgcccgctc
     3421 cgcgctgctc agcgacgtgg tgctgtacga agagcatccc gcgcgctacg cgggggacgt
     3481 cgcccgccgg catcgctgga tgcgcggcga ctggcagatc gccccgtgga tcttgccgcg
     3541 ggtgccgggg cggtcgggca agcgcgtccg caacccgatc tccgccttgt cccaatggaa
     3601 aatcttcgac aacctgcgcc gctgcctggt gccggtggcg atggtgctct tgctcgtcat
     3661 ctcctggctg ctgtcgccgg ccatggcgac cgccgcgctc gtatttgtgg ccaccgtggt
     3721 cgcgttgccg ccgctcttga ccgccgcggc tgatctgctg cgcaagccgg cggatttgcc
     3781 cctgggaatg cattctcgcg cgacgctcca catgctcgcg cggtcgctgg ctcaaggggt
     3841 gctgttgtgc gtcttcctgc cttacgaagc tttcgtcagc gccgatgccg tcgtgcgcac
     3901 gctggtgcgg atgacgtgga ccaaacggcg gctgctggaa tggaagaccg ccagcgattc
     3961 ggaacgcggc gccgatggca gcctcgaaga gacctaccgc acaatggcct ttgcccccgc
     4021 cttgggcgca tcggcattgc tactgctggt cttttacgat cgccaggtgc tgctccatgc
     4081 gggtccctgg atcgccgcct ggatggcgtc tcccttcatc gcctggtggc taagccagcc
     4141 gttcccccgg caggagctgc aattctcgga caaccagcgg cactttctgg agaagctgtc
     4201 gcggaaaacg tggcgctact tcgaggattt cgtcacggcg gaggacaact ggctcccccc
     4261 ggacaacgtc cagcagaatc ccggcctggc catttctccg cgcaccagtc cgacgaacat
     4321 cggcatggcg ctactggccg acctggcggc atacgatttc ggctactgct cggccgcacg
     4381 gctgctggcg cggacgaagc gcaccttgga aacaatgttg ctgctggaac gctatcgggg
     4441 gcattttctc aattggtatc aaacgcggac cctggctccc ttgcatccgc agtatgtttc
     4501 cacggtcgat agcggcaatc tggcggccag cctgctagtg ctcggcagcg gctgcctgga
     4561 actgagcgag actcgcatcc tgccgccgcg ctggagcggc ggcctgcgag atacgctgcg
     4621 cgtgctgttg gacgccgccc acggcacggc cggcccgccc gtcagtgccg acgtgctgcg
     4681 caagatggag cgccagattg acgaacttga gcaggccccc gcgacgttgg ccgccgcggg
     4741 cgcgctgctc tcgcaattga gcgtcgccgc cgccgaattg actgccgtgg cgggaagcga
     4801 cgcggaggtc tcctggtggg caggcgccta cgagttgtct tgcaccgacc atcgcaccga
     4861 cctcaatcat ctggcggcgt ggcttgccct gccggctgct ccggcgggca cgtgggaatg
     4921 cgactgcccc aagcagcgcg aacatctaag caagttgcgc cagatgctgg acggcctcga
     4981 tcgcgcggcg acgcttcccg acgtggctgg cttgcagcag acggcgctgc cgctgatcga
     5041 cgcggccttg ccgacggccg ccgccgccgc gaaacagtgg ctcgcgcagc tacggaccgc
     5101 gctcgccgat tccgcggaac atgctgccgc gcggatccac gccttgaagg aggtggccga
     5161 gaagtgccgc gagctggccg atatggattt cagcttgctg tacgatcccg ggcgggagct
     5221 attctccatc ggttacaacc tcagtgagcg gcggctcgat gcgagctttt acgatctgct
     5281 cgcctccgaa gcgcggctgg ctagcttcgt cgtcatcgcc caggggcatt tcggacagga
     5341 gcactggttc gcacttggcc gactgctcac cagtgccggg agcgtgccga ccctcctgag
     5401 ctggagcggg tcgatgttcg agtacttgat gccgctctta atgatgccga cgtacgaaaa
     5461 tacgctgctc gaccgcacct atcatgcggc ggtacgccgg cagatcgagt acggccgcca
     5521 gcgaggagtg ccgtggggca tctccgagtc aggctacaac acgttcgatc agcacaagac
     5581 gtatcagtac cgggccttcg gcgtgccggg gctggggctc aagcgggggc tggtggaaga
     5641 tctcgtcatc gccccgtacg ccagcgcgct ggcactgatg gtggcgccgg aagcagcctg
     5701 ccgcaacctc gagcggctga cggccgacgg ccaccaaggc ccctacggct tctacgaagc
     5761 ggtcgattac accccctcgc ggttgcctcc cggagtcgag cgggtcaccg tgcggcagtt
     5821 catggcgcat caccagggga tgagcctgct atccctggcc tacgtcctgc tcgagcggcc
     5881 gatgcagcgc cgctttctgg ccgacccgat gctgcgggcg gccatcctgc tgctgcaaga
     5941 gcgagttccc accgcggtgg caccggttta tccccacgcc actgaagcga gcgccacacg
     6001 gctggcgtcg ggagaagaaa cggggagcat gcgcgtcttc actgacccca gcagcgcgac
     6061 ggtggaagcc cacttgctct ccaacggccg ctatcacgtc gccgtgacca acgccggcgg
     6121 cggctacagc cgctggtgcg acgtggcggt gactcggtgg cgcgaggatg cgacgcggga
     6181 cagctacggc agcttctctt acctgcggga tatagacagc ggcgcagtgt ggtcgagcac
     6241 ccatcatcca acgacgacgc ggacgaaggg ctatgaagca atcttcaccc agtcgcgggc
     6301 ggagtttcgc cggaccgacg aagagatcga aacctacacg cagatctgcg tctcccccga
     6361 agatgacatc gagcttagac gcatcacgct gacgaaccgc ggagagttgc cgcgcacgct
     6421 cgagattacc agctatgccg aggtcgtgct ggcggcgcag gcgcaggacg aggcgcatgc
     6481 cgcgttcagc aatctgttcg tgcagaccga gttggtcccc ggctgccagg cgatttatgc
     6541 gacgcgacgg ccgcgctccg ccgaagagcg tcccccgtgg atgacgcaca tgatggcggt
     6601 ccgcggagtc acgatcggcg aaccgtccta cgagaccgac cggatgcggt tcatcggccg
     6661 gcaccggacg ctcgcctccc cagcggccct cgatcgtgcg acgccccttt ccaacaccgc
     6721 cgggccggtc cttgatccga tcgtcagcat ccgccaggtg gtgctaatca tgcccaacga
     6781 gtcggtgcgg atcgacatca tcacaggcgt cgccgaatcg cgtgctgcgg tcgatgggct
     6841 gaccgaaaag tatcgcgatg ccagcctggc cgaccgcgtg ttcgacctgg cctggaccca
     6901 ctcccatatt ctgctgcagc agcttagcgc ctccgaagcc gaagcgcagg tctatggtcg
     6961 cctcgccggc tcgatcattt acgcctccgc cctgcggcgg gccaaagcca gcatcctcgc
     7021 ccacaatcga cgcggccagt cggggctgtg gggctacggc atttcgggag atctcccgat
     7081 cgtcctggta cggattcgcg accgccagcg gatcggcctc gtgcaacagg cggtccaggc
     7141 gcacgcctac tggcggctga aggggttggt ggtcgatctg gtgatctgga atgaagacga
     7201 ttcggtctat cgacaggaat tgcagcaggc catcgtcgat ctggtcgccg ccagtccgga
     7261 agcgtcgctc gtcgaccgac ccggaggcat tttcatccgc cgcggcgagc agatgtcgga
     7321 cgaagaccaa gcgctcatgc aaaccgtagc tcggatcgta ctcgtggacg aggctggaac
     7381 gctggcggaa caggccgagc gccgtggccg gtccgacgta tcgatcccgc cgctcaagcc
     7441 ggttcgccgc cagagcgaac cggtatccta cgaacccccc cagcgcgacc tggcgttctt
     7501 caacggcctc ggcggcttca gccacgacgg ccgcgaatac gtcaccatcc tcgcccccga
     7561 ccagacaacc ccggcgccgt gggtgaacgt gatcgccaac gcgcagttcg ggactgtcgt
     7621 gtcggaggga ggaagcgctt acacctgggc ggaaaacagc cgcgagttcc ggctcactcc
     7681 ctggcgcaac gaccccgttt ccgacatcag tggcgaagcg atttacctgc gcgacgaaga
     7741 gtcggggcgg ttctggtctc cctcgccgct gccggcccgc gggaaaaaca cctacatggt
     7801 ccgccacggt ttcggctaca ccattttcga ttacaccgag gaagggctga cgaccgagtt
     7861 gtgcgtctat gtggcgaccg acgcgccggt gaagttttac aagctcaaga tcgccaaccg
     7921 ctcgggccgc acgcggcagt tgtcggtcac gggctattgg gagctggtcc tcggtgactt
     7981 ccgcagcaag acgctgctgc acgtcgtgac cgagtccgat ccgacgagcg gcgcgatcct
     8041 cgcccgcaac gtttacagcc cggagttcgg ggaccgcgta gcgtttttca attgcagcga
     8101 aatggcacgc accttcaccg gcgaccgcgc cgaattcctg gggcgcaacg gccagttcgc
     8161 cagcccatcg gcaatgcggc gcgtgcggct ctcggggcgc gtcggcgcgg gatacgatcc
     8221 gtgtgccgcc tttcaagcgc cgctgacgct cgccgacggg caagagcgaa tcatcacgtt
     8281 caccatcggt gccgcaggcg gagcggcgga ggccagatca ctggcgcagc ggtatcgcag
     8341 cgtggacagc gcccatcggg cgatcgaggg cgtgtgggac tactggagcc ggacgctggg
     8401 ggtcgtctat ttggaaacgc ccgatcccag ggtcaattac ctggccaacg gatggctcat
     8461 ctatcaaacg ctggcctgcc ggatgtgggc ccgcaccggc ttttatcaat cgggaggcgc
     8521 gtatggcttc cgggatcagc tccaggacgc gatggcgctg gtccatgcgc agccgacgtt
     8581 gctccgcgag catctgctgc gtgccgcctc ccgccagttc cgcgaagggg acgtgcagca
     8641 ttggtggcac ccgccgctgg gacggggagt gcggacccat ttctcggatg atttcctgtg
     8701 gctgccgttg gcgatctgcc gctacgtcgg catcacgggg gacaccggcg tgctggacga
     8761 gcgcattccc ttcttgaccg cgcgcctcct tcgcgacgac gaggagtcta attacgacct
     8821 gccgcagatt tcgcacgaaa tcggcacact ctacgagcac gcggtccgcg ccatcgatca
     8881 cggtttgcgg ttcgggcagc acggactgcc gctgatgggc agcggcgact ggaacgacgg
     8941 catgaacctg gtcgggcacc gcggcaaggg agagagcgtg tggctggcgt ttttcctgtt
     9001 tcaggtgctc acgcagtttg ccaagttggc gcggcggcgc ggcgacgtga cgctggcgga
     9061 tcggtacacg gtcgaggccg gccggctgcg cggcaacatc gaggagaacg gttgggacgg
     9121 gcagtggtat cgccgggcct atttcgacga tggcacgccg cttggatccg ccgccaacga
     9181 agaatgccag atcgacgcga tttcacagag ctggtcggtg ctctcggggg ccggcacgcc
     9241 cgagcggagg atcgcggcgc tgtcgagcgt cgacaagcgg ctcgtccgcc gcgatgcccg
     9301 cttgatcctg ctgctcgatc cgccgtttga caaatcaaac ctcgaccccg gttacatcaa
     9361 agggtacgtc cccggcgtcc gcgaaaacgg cggccagtac acgcacagcg ccatttggac
     9421 cgtgatggcc atggcggcga tgggggaccg cgagcgggcc tgggagctgt tcagcctgat
     9481 caatccgatt tcgcacgggg ccacggccga cgacatcgcg acttaccgcg ccgagcccta
     9541 tgtggttgct gccgacgtct acggcgtcga tccgcatacc ggccgcggcg gctggacctg
     9601 gtacaccggc tcggccggct ggatgtatcg gctgatcgtg gaatcgctcc tcggtctgca
     9661 cctggaggtg gacaagctgc ggttcgcgcc gtgcctgccg gcacagtgga cggagttcaa
     9721 gattcactat cgctatcgcg agactttcta tcacatcacc atccgcaacg atgccgcggg
     9781 agggacggtc ggacgcctgg tgctggatgg ggctgaatta tccgacgatt tcgtcaccct
     9841 gatcgatgat cgtcaggacc acgaattgga agtggaactg cgtcctgacg atatttcgac
     9901 gcagccgatt ctcggtggtg actagaggtg gatcttccga cgtccgctca ccgcatcgtt
     9961 caatacgttt tgcccctacg ttcgcagccc tctctttagg cggggcaagc gtgaattatg
    10021 tttaccgcaa cgcacattgg ctctcgattt accgcgtttg tgaccgacgc gtcttggagt
    10081 aaaggcttgg gcatcaagcc gaagagaaac atggagacgg gggaggacga aacgtgggag
    10141 ctcaaggatc tccggaagac ttgtgcaacc ttttacgacg agcacgtccc cgaatcttcg
    10201 atcgagattc tcggccattc cgtgagcggt gtgacatacc gccactacgc ccatcgcgcc
    10261 cccttggcgt tcaaggcgat catgacgctg ccccagccgt cggccttcac gggcttggtc
    10321 cgtggcttcg acggtcaatg cccgtgctgc cggcggcctt tcgcagatcg atcgtagacg
    10381 cgggcgatgg gaccga
//
LOCUS       DASUGB010000428         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_882879, whole genome shotgun sequence.
ACCESSION   DASUGB010000428 DASUGB010000000
VERSION     DASUGB010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882879"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFV87_08295"
     CDS             <1..187
                     /locus_tag="VFV87_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5103794.1"
                     /translation="GKHPRGHAGASEEHTELIRLRREMDDAKQKEEYERAARLRDQIR
                     ELEKKMRPPAGEGQGVA"
     gene            189..1289
                     /locus_tag="VFV87_08300"
     CDS             189..1289
                     /locus_tag="VFV87_08300"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX5103795.1"
                     /translation="MAKSFSVKLEELANRCGEWLRGSGPQSEIVISSRIRLARNLADY
                     PFIRRCTEADRLAVEKAFKEAAATVPDFKELSYVPVSELAILDRQFLVERQLISRELA
                     DANGARGVYFDANETFGFMINEEDHLRMQVMHSGLDMDAAWEQINRMDDLLSERLNYA
                     FHEKLGYLTACPTNVGTGMRVSVMLHLPALVITQQIEKVFRSLQKMGLAVRGLYGEGS
                     QAMGDFYQISNQVTLGRSEIDLVKQVGEVIPVIIDYERQARAFLVKESKKDLHDRVSR
                     AYGILCTAQTISSEETLHLLSSVRMGIYLGLIQDLEIPTVNKLFIHTQPAHLQKLRGA
                     ELPSADRNVERAMYLQRHLQKRQNGGPEVGNN"
     gene            1445..>2095
                     /locus_tag="VFV87_08305"
     CDS             1445..>2095
                     /locus_tag="VFV87_08305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5103796.1"
                     /translation="MRFAFLAVSFVSVCLVAPLFGAESLDPAQAKEENGALWYDVRLL
                     TVEGQGWSAADLKAPYDRLPAKAEGKVRDAVWGLSRDSAGLCIRFRTDSPTIQCRWTL
                     TKSNLAMPHMAATGVSGVDLYVNVDGWRWLATGRPTSQLNSAALVSNLPAGKRAYVLY
                     LPLYNGVSSVEIGIANGSDLEALPRDEKRAKPIVFWGTSITQGGCASRTGMVHTAIL"
BASE COUNT          415 a          636 c          668 g          376 t
ORIGIN      
        1 cggcaagcat ccgcgcggcc atgccggagc ttccgaggag cacaccgagc tgatccgcct
       61 gcggcgcgaa atggacgacg ccaagcagaa agaggagtac gagcgggcag ccaggttgcg
      121 cgaccagatt cgcgaattgg agaagaagat gcgtccgccg gctggcgaag gccaaggagt
      181 ggcgtgacgt ggccaagtcg ttttctgtga agctcgaaga actggcaaac cgctgcggcg
      241 agtggctccg cggcagcgga ccgcagagcg agatcgtcat ctccagccgc atccgcctgg
      301 cccgcaacct ggccgattat ccgttcatcc gccgctgtac cgaggcggac cgcctggcgg
      361 tcgaaaaggc cttcaaggaa gcggcggcga cggtgccgga cttcaaagag ctgtcgtacg
      421 ttccggtttc ggagctggcg attctcgacc ggcagttcct cgtcgagcgc cagctcatca
      481 gccgcgaact ggccgacgcc aacggcgccc gcggcgtcta tttcgacgcc aacgagacgt
      541 tcggcttcat gatcaacgag gaagatcacc tgcggatgca ggtcatgcac agcggcctgg
      601 acatggacgc cgcctgggaa cagatcaacc gcatggacga cctgctcagc gagcggctga
      661 attacgcatt ccacgagaag ctgggctatc tgaccgcttg cccgacgaac gtcggcaccg
      721 gcatgcgcgt gagcgtgatg ctgcacctgc cggccctcgt gatcacgcag cagatcgaaa
      781 aggtcttccg cagcctgcag aagatgggcc tggcggtccg cggcctgtat ggcgaaggct
      841 cgcaggcgat gggcgatttt taccagatca gcaaccaggt gacgctcggt cgcagcgaaa
      901 tcgatctggt caaacaggtc ggcgaggtga ttccggtcat catcgactac gagcgtcagg
      961 cgcgggcttt tctcgtcaag gaaagcaaaa aggacctgca cgaccgcgtc agccgggcgt
     1021 acggcatcct ctgcacagcg cagaccattt cgagcgaaga gacgctgcat ctgttatcga
     1081 gcgtgcggat ggggatctac ctcggtctga tacaagacct tgaaatcccg accgtcaaca
     1141 agctcttcat ccacacgcag ccggcccacc tgcagaagct ccgcggcgcc gagttgccca
     1201 gcgccgaccg caacgtcgag cgggccatgt atctgcagcg gcacctgcag aagcgccaga
     1261 acggcgggcc ggaagttggg aacaactaac agtcgcatct tcgcaccatt gtggccggca
     1321 gatccgcttg ccacagtgcg ccgctggaat gggtacgtgc tgcgtgcaaa cctttgcgcg
     1381 gtctgctagg cttccccccc aagaatggac agtttctgct ccatcatctc ctgggagttt
     1441 tgcaatgcgt tttgctttcc ttgccgtgtc gtttgtgtcg gtatgtttgg tggcgcctct
     1501 atttggcgct gaatcactcg atccagccca ggcgaaggag gaaaacggcg ccctgtggta
     1561 tgacgtgcgc ttgctgaccg tggaaggtca ggggtggagt gccgccgatc tgaaggcgcc
     1621 ctacgatcgt ctgccggcca aggccgaggg caaagtccgc gacgccgtat ggggcctgtc
     1681 gcgcgattcg gccggccttt gcatccggtt caggacggat tcgcccacca ttcagtgccg
     1741 ctggacattg acgaagtcga acctggccat gccccacatg gcggcgacag gcgtcagcgg
     1801 cgttgatttg tacgtcaacg tggacggatg gcgttggctg gcaacgggcc ggcctacgag
     1861 ccaattgaat tctgcagccc tggtgagcaa tctgccggcc ggcaaacggg cttacgtgct
     1921 gtacctgccg ctctataacg gtgtgtccag cgtggaaatt ggcatcgcca acggcagcga
     1981 cttggaggct ctgccccgcg acgagaaacg cgccaagcca atcgtgttct ggggcacgtc
     2041 aatcacgcag ggtggttgcg cgtcgcgcac aggcatggtt cacacggcaa tcctc
//
LOCUS       DASUGB010000429         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_743475, whole genome shotgun sequence.
ACCESSION   DASUGB010000429 DASUGB010000000
VERSION     DASUGB010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743475"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFV87_08310"
     CDS             <1..485
                     /locus_tag="VFV87_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5103797.1"
                     /translation="KGIANGAPLGACVTRHQIAAVMQRRLHFNTFGGNPISVTQGLAT
                     LEIIDREQIQANAREVGSYLKQRLLEVQDRQPLIGEVRGMGLMLGVELVRDRQTKEPA
                     DREAAEVLELCKDRGLLIGKGGLYGNTLRIKPPMCITKDDADFLADCLDEALTSVAKT
                     "
     gene            537..1964
                     /gene="mmsA"
                     /locus_tag="VFV87_08315"
     CDS             537..1964
                     /gene="mmsA"
                     /locus_tag="VFV87_08315"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5103798.1"
                     /translation="MKYPPVQNFIGGLFASPIGEHVPLVSPLNGAAISQVPQSGAAEV
                     AAAVAAAKAAFPAWSGQTLKQRANVMYRYRELLVKHTGEIAERIHVENGKTLDEARAE
                     IVRAIEVTEFACSLPQLAAGEVLEVSPGVECRVDRLPLGVVASIAPFNFPMMVPHWTI
                     PIAITLGNTLVFKPSDKVPLSAVRTAELLAEAGLPPGVFNVVHGERAAVEAICDHPDI
                     KAVTFVGSTPIAKAVYIRATSQLKRALCLGGAKNHLIVLPDAAIQATAENAVASMAGC
                     AGQRCMAAASLLAVGDVEAVIDEICHAARRLIPGKNLGPVISAAAKSRIEGYIDQAEA
                     AGAKILVDGRGVVVAGCENGFYIGPTVIDHVQPEMKIAQEEVFGPVLAILRATDVDEA
                     LQIENASPYGNAAAIYTKSGQQARTIGQRASAGMIGVNIGVPVPVEPFGFGGWNDSKF
                     GVGDITGKNSIEFWTQAKKTTTRWV"
     gene            complement(2060..3340)
                     /locus_tag="VFV87_08320"
     CDS             complement(2060..3340)
                     /locus_tag="VFV87_08320"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX5103799.1"
                     /translation="MGEHDTGFQISHSNLRFGSGTTREVGLDLEDMGLRRTLLVIDPK
                     LRELPTGQTVVESLRSARREFEIFDAVETEPTDQAFHAAIEFAGRGRFDSFVAVGGGS
                     TIDTAKAANLYSTYPADFLDYVNAPIGRGLPVRGPLKPLIAIPTTAGTGSETTGVAIF
                     DLVQRKAKTGIAHRYLRPTLGIVDRDNTRTQPPAVAASAGLDVLSHALESFTAIPYDE
                     RPRPERPILRPAYQGTNPISDMWAIRALEIVAEFLPRAVTDTSDDEARGQMLLASSMA
                     GIGFGNAGVHLPHGMSYPVAGMVKNFRPPGYEVDHPLVPHGMSVILHTPAVVRFTAPA
                     CPERHLRAAAALGADIRGAAPEDAGEILAQRVIDFMRLLGMPTGLAAVGYSEADIPAL
                     VQGTLPQHRVTKLSPRPAGEAELTELFRQSLRIW"
     gene            3412..4437
                     /locus_tag="VFV87_08325"
     CDS             3412..4437
                     /locus_tag="VFV87_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX5103800.1"
                     /translation="MRQILTIALVFTAASVLQAADSAVPTYPGPNRPDEPLRKEFSLA
                     AAADFLDAASADWWQTRGCFTCHTNYSYLLARPGLKTESPVATQMRQHLEELVEQRWA
                     EKGPRWDAEVIMSGAVLAQHDAATTGKLHETARKALDRMWTVQREDGGFNWLKCNWPP
                     MESDDHFGATVALIGVGAAPDDYRSTPAAVKGLEQLKKYLAANPPPTLHHRLMVLWAD
                     SYKLDLTTDGERAAWLDELTKLQRDDGGWNIASFGDWKRGDDTAQDTTASDGYATGLA
                     IFILRRMGTPANDPRIVRGIAWLKANQRESGRWYTRSLFKDNKHYLSHAGTAMAVLAL
                     TSCGISD"
     gene            4588..>5074
                     /locus_tag="VFV87_08330"
     CDS             4588..>5074
                     /locus_tag="VFV87_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103801.1"
                     /translation="MSIASLHPLLKLGLGAVLVVAGAAAIQAVAWVLGLDFSILQSGN
                     GGRGVLLAVAIATLLFLMGSDRRPIADYGLAVGPDWKWRLLRGIAVGVLTYSAYLTLC
                     WLGGAYVLSPQSVPAGRWLSALLSALPSVILAVTQQFIFSGYILSMLKDRLRPTVAVA
                     LA"
BASE COUNT          912 a         1683 c         1619 g          860 t
ORIGIN      
        1 ccaagggaat tgccaacggc gcgccgctcg gcgcgtgcgt cacgcggcac caaatcgccg
       61 ccgtgatgca gcgccggctg cacttcaaca ccttcggcgg caatccgatc agcgtcacgc
      121 aagggctggc gacgctcgaa atcatcgacc gcgaacagat ccaggccaat gcccgcgaag
      181 tcgggagcta cctgaagcag cgactgctgg aggtccagga ccgtcagccg ctgatcggcg
      241 aagtccgcgg catgggcctg atgctcggcg tcgagctggt tcgcgatcgc cagacgaaag
      301 agcccgccga tcgcgaagcg gctgaggtgc tggagctgtg caaggaccgc ggcctcctga
      361 tcggcaaagg cggcctctac ggcaacacgc tccgcatcaa gccgccgatg tgcatcacca
      421 aggacgacgc ggatttcctg gcggactgtc tggatgaggc gctgacatcg gtcgcgaaga
      481 cgtagggtgg gaccagcgag cgcagctcgc acaggcccac caagattgag gaacgcatga
      541 aatacccacc cgtccaaaac ttcatcggcg ggctgttcgc atctcccata ggtgaacacg
      601 ttccgctcgt ctcgccgctg aatggcgcgg caatctcgca agtgccgcaa tcgggtgcgg
      661 ccgaagtcgc cgcagccgtt gccgcggcca aagcagcgtt tcccgcgtgg tcgggccaga
      721 cgctgaagca gcgggcgaac gtcatgtacc gctatcgcga gctgctggtc aagcacacgg
      781 gcgaaatcgc agagcgaatt catgtggaga acggcaagac gctcgacgag gcccgggccg
      841 agattgtgcg ggcgatcgag gtgacagagt tcgcctgctc gctaccacag cttgcggcgg
      901 gcgaggtgct ggaggtcagc ccgggcgtgg aatgccgcgt cgatcgtcta ccgctgggtg
      961 tcgtcgcgtc gatcgcgccg ttcaatttcc cgatgatggt cccgcactgg acgatcccca
     1021 tcgcgatcac gctcggcaac acgctcgtct tcaaaccgtc cgacaaggtc ccgctctcgg
     1081 ctgtccgcac cgcggaattg ctggccgaag ccggcttgcc gcccggcgtg ttcaacgtcg
     1141 tccacggcga gcgggccgcg gtcgaagcga tctgcgacca tcccgacatc aaggcggtca
     1201 cgttcgtcgg ctcgacaccg atcgccaaag ccgtctatat ccgggcgacg agtcagctca
     1261 agcgggccct ctgccttggc ggggccaaga accatctgat tgtcctgccc gacgcggcga
     1321 ttcaggccac tgccgaaaac gctgtcgcct caatggccgg ctgtgcaggg cagcgatgca
     1381 tggccgcagc ttcgctgctg gcggtgggcg acgtcgaagc ggtcatcgac gaaatctgcc
     1441 acgcggctcg ccgcctcata cccggcaaga acctcggccc ggtgatttcg gcagccgcca
     1501 agtcgcggat cgaaggctac atcgaccagg ccgaagctgc cggggcaaag atcctcgtcg
     1561 atggccgcgg ggtcgttgtc gcaggctgcg aaaatggctt ctacatcggc ccgacggtga
     1621 tcgaccacgt gcagcccgaa atgaagatcg cccaggagga agtttttggc ccggtcctgg
     1681 cgatcctccg cgccacggac gttgatgagg ccctgcaaat cgagaacgcc tccccctacg
     1741 gcaatgccgc ggccatctac accaagagcg gacagcaggc tcgcacgatc ggccagcgag
     1801 ccagcgctgg catgatcggc gtcaacatcg gcgtccccgt ccccgtcgaa cccttcggct
     1861 tcggcggctg gaacgactcc aagttcggcg tgggagacat caccggcaaa aactcgatcg
     1921 aattctggac gcaggcgaag aaaacgacca cgcgatgggt gtagggtggg acaaggccga
     1981 caggccgcag gcccaccatc cgtttggcgt gcagattgac cgtggcgggc ctgcgctcgc
     2041 attgctcgct ggtcccaccc taccaaattc tcagcgactg ccggaacaat tccgtcaact
     2101 ccgcctcgcc cgccgggcgc ggcgaaagtt tggtcactcg gtgctgcggc agcgtgcctt
     2161 ggaccagggc cgggatgtcg gcttcggaat agccgacggc ggcgaggccc gttggcatgc
     2221 ccagcagccg catgaagtcg atgacccgct gagcgaggat ttcgcccgcg tcttcgggag
     2281 cggcgccgcg gatgtcggcc ccgagagcgg ccgcagcccg cagatgccgc tcgggacatg
     2341 ccggcgcggt gaaccgcacg actgccggcg tgtgcaaaat gaccgacatc ccgtgcggca
     2401 cgagcggatg atcgacctca tagcccggcg gtcggaagtt cttcaccata ccggcgaccg
     2461 gatacgacat gccgtggggc aggtggacgc ccgcattgcc gaagccgatg cccgccatcg
     2521 acgaggccag cagcatctgc ccgcgggcct catcgtcgct ggtatcggtg accgcccgcg
     2581 gcaggaattc agccacaatc tccaaggctc gaattgccca catatcgctg atcggatttg
     2641 tgccctgata tgccggtcgc aggatcggcc gctcggggcg cggccgctcg tcgtacggaa
     2701 tcgccgtgaa cgattccaag gcgtggctga gcacgtcgag cccagccgac gccgccaccg
     2761 ccggcggctg cgtgcgggtg ttgtcgcgat cgacaatccc cagcgtgggc cgcaagtagc
     2821 gatgggcgat cccggtcttg gccttccgct gcaccagatc gaagatcgcc acgccagtcg
     2881 tctcgctgcc ggtgccggcg gttgtgggaa tcgcaattag tggcttgagc ggcccccgca
     2941 cgggcagtcc gcggccgatc ggcgcgttga cgtagtcgag gaaatccgcc gggtacgtcg
     3001 agtacagatt cgcggccttg gccgtgtcga tcgtgctccc gccgccaacc gcgacgaacg
     3061 aatcgaaccg gccgcggccg gcgaactcaa tcgccgcatg aaacgcctgg tcggtgggct
     3121 ccgtttcgac cgcgtcgaag atttcaaact cgcgccgcgc ggagcggagc gattcgacca
     3181 ccgtctgtcc cgtcggcaat tcgcggagct tcggatcgat caccagcagc gtccgcctca
     3241 ggcccatatc ctccaggtct aaacccacct cccgcgtcgt cccgctgccg aagcggaggt
     3301 tcgagtgtga aatctgaaat cccgtgtcat gctcgcccat gtggatagcc gcctcccgat
     3361 gcgagtatat tcgcacatgt gatctggcgg ctctacctgg aggaatgctc gatgcggcaa
     3421 attctcacta tcgcactcgt attcaccgct gcgtccgtac ttcaggctgc cgattccgcg
     3481 gtccccacct atcccggccc caaccgcccc gacgaaccgc tgcggaagga gttctcgctg
     3541 gccgcggcgg cagactttct tgacgcggcc tcggccgact ggtggcagac ccgcggctgc
     3601 ttcacttgcc acacgaacta cagctatctc ctcgctcggc cgggcctcaa aacggaatct
     3661 cccgtggcca cgcaaatgcg gcaacacttg gaggaattgg tcgagcagcg gtgggccgag
     3721 aaaggcccgc gctgggacgc cgaagtcatc atgagcggcg ctgtcctcgc tcagcacgac
     3781 gcggccacga ccggcaagct gcacgaaaca gcccgcaagg ctctcgaccg gatgtggacg
     3841 gtgcagcgtg aggacggcgg cttcaattgg ctcaagtgca actggccgcc gatggagtcg
     3901 gacgaccatt tcggcgcgac ggtggcctta atcggcgtcg gggccgcccc ggacgactat
     3961 cgcagcacac ccgccgcggt gaaaggcctt gagcaactga agaaatacct cgccgccaac
     4021 ccgccgccga cgctgcatca caggctgatg gtcctctggg ccgactccta caaactggac
     4081 ctgaccactg acggcgagcg tgcagcctgg ctcgatgaac tgaccaaact gcagcgcgac
     4141 gacggcggct ggaatattgc ttcgttcggc gactggaagc ggggcgacga cacggcgcag
     4201 gacaccaccg ccagcgatgg ctacgcgacc gggctggcca tcttcatcct tcgccggatg
     4261 ggcacgcccg ccaacgatcc gcgcatcgtc cggggaatcg cctggcttaa ggcgaaccag
     4321 cgcgaaagcg gccgctggta cacgcgctcg ctcttcaagg acaacaagca ctacctcagc
     4381 catgccggca cggcgatggc cgtgctggca ctgacgtcgt gcggcattag cgattgatgt
     4441 gggctgcgcc aatctttgcc agattgccaa agcgaactga tcttgccctg ttccttcgct
     4501 ggcaattaga ttccgcgcga tgcttccagg acgcagccgc tatggcctgc gtgctcacct
     4561 tgctccgctt gcactccggc gacgactatg tccattgcct cgctgcatcc gctgctcaag
     4621 cttggactgg gtgcggtgct ggttgtcgcc ggagctgcgg caatccaggc agtcgcctgg
     4681 gtcctggggc tggacttcag catcctgcaa tcgggcaacg gcggccgggg cgtgctgctg
     4741 gcggttgcga tcgcgacgct gctcttcctg atgggttccg accggcggcc gattgccgac
     4801 tacggtctgg ctgtcgggcc ggactggaaa tggcgactgc taagaggcat cgcggttgga
     4861 gtgcttacct attctgcgta cctgacgctg tgttggctag gcggcgcgta tgtgcttagt
     4921 ccgcagtccg tcccggccgg ccgttggctc tcggcccttc tctccgcgct accgtcggtg
     4981 atcctggcag tcacgcagca gttcatcttc agcggctata tcctcagcat gctcaaagac
     5041 cggttgcggc caaccgtcgc cgtagcgctg gccg
//
LOCUS       DASUGB010000430         9410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1578109, whole genome shotgun sequence.
ACCESSION   DASUGB010000430 DASUGB010000000
VERSION     DASUGB010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578109"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFV87_08335"
     CDS             <1..331
                     /locus_tag="VFV87_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5103802.1"
                     /translation="ENTAPYRVDLRRAEYGDERDAKMREVFERISPANHADKIKSALL
                     VAHGRNDPRVPFSEAEQIAAKVRDQGRTVWTVFADNEGHGFGKKDNADYLRTVEALFL
                     KQSLAIP"
     gene            complement(406..2400)
                     /locus_tag="VFV87_08340"
     CDS             complement(406..2400)
                     /locus_tag="VFV87_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103803.1"
                     /translation="MRFSSVRLLSVALVISFATRGLAVQPSESLLPATTKGFISTHDV
                     DEVRNKFNETQLGEMAADPVMKPFIDDLRKQIGAKLEKAGKRLGLKWKDMEGVYAGES
                     TLALIQPDPKDKMSHATVLIVDITGKRDKADEMLAKVDKNQKEGKAVRTAHAFGGVTM
                     TVYTQPLKAGEKVAEQSYYFIKDDTLVISDHKAVSEGIVGRFGGQATDSLATVEAFKY
                     TMARNVKEAAGVRHHVRWFVEPFGFAEAHRASQGGRKKRGTDLLKILPTQGFNAIQGV
                     GGYVFFATGSEEVLHRTYVYAPAVKRDPAKPSKDKYDLAMRMLNFPNGGDLEPQKWVL
                     SDVGGYLTFNGKLQDAFKYSETLVDAIAGDKGVFEEIWNSMKHDPNGPKIDIYAELIN
                     HLGERASLLTDITVPVHLKSERQMAFIEVKDAAIVARTLEKAFKDDPAAKKHTYRGHI
                     IWEIQQEETLTEEPELVIEGAGFVNAEKKKKGKDDEEEEPKLPNMALTVFEGHLVVST
                     HMSFVEEFLGRAKDPNNLAKMDDFKRVHAALTKRGMAAADTSIRYFSRTDETFHHTYE
                     MLKQGKLPETESFFARILNAMLSEDEDEVRKPEIDGSKLPEFELVKKYLGPGGIFAVQ
                     ENEGWYLVGTLLKKEVPLVAKAPAAAATEASVDSQGSLQE"
     gene            complement(2707..4269)
                     /locus_tag="VFV87_08345"
     CDS             complement(2707..4269)
                     /locus_tag="VFV87_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5103804.1"
                     /translation="MSTPESLIHRYLDDRSSLDAAELDELIALLRAEPARAAALREQL
                     LLDDLLAQKLAVDRRIFQAQVGQRIADFHRGQAEMDDQVSELRALAESEFERPTTWSG
                     SSPWVKYIALAATVLIAAVIFVPRWLPRTPQPLAKVTAMQGDVQVAGQPAAIGVGRAV
                     LSGQQIVTPDGASLAIEYADKTTVQIAGGSTVTLDIDDATGGKRVAIEVGAITAVVSP
                     QGAGAPMKLTTPHAVATVVGTEFRLTVSATTTLLDVTEGKVLLDRLADKQSIAVGANE
                     TGLASSELLQVRPLAWPEDHRNVAYIYTPFDFLTEFAPVSASRNPMTSSLRDTPLELS
                     GATTVNEFTSAVEFQGGHARSDDAGVDLLASLSDKSELTIEIVFSATTAEQEGPARIV
                     ALADDGQAANFALLQDGQRLGFQLLTDAGDVGETIWVAPATPTQTSHIAIAYRDGSLV
                     IYRDGEKVTESDKFQGSFSMWRGGPLTLGADASGQSPWLGTIEAIAIHSRCLEEDEIA
                     RNARSYRLLARQ"
     gene            complement(4373..4906)
                     /locus_tag="VFV87_08350"
     CDS             complement(4373..4906)
                     /locus_tag="VFV87_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5103805.1"
                     /translation="MSDQPLASLDFDVVVRDTQPHIRAYIAGMGVPRHDVDDVAQDVY
                     LELYRFMDRVPPEVTPKQWLKGIARNLCLNYFRRTGRRQRLQREALVEIFLRAEQECA
                     VSLSEGPVRRALDGCYEKLPGESRRLLALRYEQELPSHTIAERVNSTAEAVRVALFRI
                     RASLKNCITKNLAKEEA"
     gene            complement(5483..6202)
                     /locus_tag="VFV87_08355"
     CDS             complement(5483..6202)
                     /locus_tag="VFV87_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103806.1"
                     /translation="MSRAKFLIGCVVVAVTLVSMVRADDPASIEPGDQAPGVVMGVDR
                     DRIDGIPIHFILNPDVRRPAGYPAEIPLEKLEAFLNTQRVTALGCGGMTWEGASCGMS
                     SHTYASSKTPWLIDDVNISADFAETKTGTFLASFVLGRNRYGRADRKNDKLVWEKTYE
                     PEYQQRANYRVLFGNDPAQRKALLARISESFKNLDVTQFPAKRRESLALQLKPCEDDS
                     DPAVAALAKELRKALSSDLQR"
     gene            complement(6378..8102)
                     /locus_tag="VFV87_08360"
     CDS             complement(6378..8102)
                     /locus_tag="VFV87_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103807.1"
                     /translation="MTKEPEFAHASGAGAAAESVQSLVRLLYTQNPFYLIGTLLILVG
                     LQQGLGKEPGLATSGLLAVGLAGFTLLLAAIAAVIIRYGKVWDDARTILLVIVLMFFM
                     LSSSLDVLLLTQPLAGSLVLAAGLTFSIGLSQTLLAGLQIHLAPRYRRPYYLMLALLF
                     GWPVVLVWINQFGLAAARPWAVLGFPVLAALSLLTLLPAAATRRRHEPASGTPWIWPF
                     YPWSLFVFLTVGFAIRSWWLTISFEPVQGQDAYFRPYFLFPLVLAWSALVLEMGKARH
                     RAGAIAAGLLLPAAGMLLVFPGAGRSPVEVAFLGRLTHTLAAPAQLAIWSLIGFYLWA
                     WLRNVRAAEGFALVAGLAAAVIGRQTLDLESLTRPQPLVLAAVAGTLLVLAIQRQSTW
                     RAVAVGAMAAFAFRFTGGGSWEAAWFWQWHAPLIGLVAVAAVLNDEFARFLRELAWRA
                     APALAAFAAVAYPWILPAVNPMSLAVYLVLLLLASVALWRRQKEVPLLAAAIVTLAAN
                     LLVLARQVYGLLDRTQLADGLPFLAWGLAATTIALVISLLKMGLWPWTQTCLARLNFA
                     LGGKEAPG"
     gene            complement(8539..9006)
                     /locus_tag="VFV87_08365"
     CDS             complement(8539..9006)
                     /locus_tag="VFV87_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103808.1"
                     /translation="MSIRTELRRRLELGLSYWPTAELDGSRQACPKADLAGIFGAASS
                     EQLGRLRDALGTNRVVRGMFVDRTAPEGNRGCLLNQLLGWTSSEDLWKWDVDDRLVMA
                     ASRTMRRWDDGSLTPQIVLAELAAELERREAQVELPAAQAAGANVPTAAALAG"
     gene            complement(9049..>9410)
                     /locus_tag="VFV87_08370"
     CDS             complement(9049..>9410)
                     /locus_tag="VFV87_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103809.1"
                     /translation="IGELVKYVRNVTLAVQVSLANEFYDIAQRLELDYTAVRNLAQLD
                     KRLPTSHWDVPGPDGRRGLSGSCFPKDINALLALCVDLQIDAQVIAGAWATNLRVRPE
                     RDWQALVGRAVVASAAP"
BASE COUNT         1692 a         3145 c         2888 g         1685 t
ORIGIN      
        1 ggaaaacacg gctccttatc gcgtcgatct gcggcgagcc gagtatggcg acgagcgcga
       61 cgcgaagatg cgcgaggtgt tcgagcggat cagccccgcc aaccacgccg acaaaatcaa
      121 aagcgccctc ttagtcgctc acggccggaa cgacccgcgc gtccctttca gcgaagcgga
      181 gcagatcgcc gccaaggtcc gcgaccaggg gcggaccgtc tggaccgtct tcgccgacaa
      241 cgaaggtcac ggcttcggga agaaggacaa cgccgactac ctgcggacgg tcgaggcgtt
      301 attcctcaag caaagcctgg ctatcccgta gccgcgttcg ccagaacgcg ggctgaccgc
      361 acgcgcgccc gcactctggc gagtgcggct acgaacgggg cggttctatt cctgcagcga
      421 gccttgggaa tccactgagg cttcggtcgc ggccgccgct ggcgccttgg ccaccagggg
      481 aacctctttc ttcagcagcg tgccgacgag gtaccagccc tcattctcct gcacggcaaa
      541 tatcccgccg gggccgagat acttcttcac cagctcaaac tcgggcagct tgctgccgtc
      601 gatctccggc ttgcggacct cgtcctcgtc ctccgagagc atcgcgttca ggatgcgggc
      661 gaagaacgac tcggtctcgg gcagcttgcc ctgcttcagc atctcgtaag tatggtggaa
      721 cgtttcgtcg gtgcggctga agtagcggat gctcgtatcg gccgcggcca tgccgcgctt
      781 ggtcagggcc gcgtggacgc gcttgaagtc gtccatcttg gcgaggttgt tcgggtcctt
      841 cgcccggccg aggaactctt cgacgaagct catgtgcgtc gagacgacca ggtgcccctc
      901 gaacacggtc agcgccatgt tcggcagctt cggctcctct tcctcgtcat ccttgccttt
      961 ctttttcttc tcagcgttga cgaagcccgc gccctcaatc accagctcgg gctcttcggt
     1021 cagcgtttct tcctgctgga tttcccaaat gatgtggcca cgataggtgt gcttcttcgc
     1081 cgccggatcg tccttgaacg ccttctccag cgtgcgggca acaatggccg catccttcac
     1141 ttcaataaac gccatctgcc gctcgctctt cagatgcacc ggcaccgtaa tgtcggtcag
     1201 gagagaggcc cgctcgccca gatggttgat cagctccgcg tagatgtcga tcttcggccc
     1261 attcgggtcg tgcttcatgc tgttccagat ctcctcgaac acgcccttgt cgccggcgat
     1321 cgcgtccacc agcgtctcgg aatacttgaa ggcgtcctgc agcttcccgt tgaacgtcag
     1381 gtagccgccc acgtcggaca gcacccactt ctgcggctcc aggtcgccgc cgttggggaa
     1441 gttcagcatc cgcatcgcca ggtcgtactt gtccttgctg ggcttggcgg gatcgcgctt
     1501 caccgccgga gcatagacgt acgtccggtg cagcacttcc tcgctgccgg tcgcgaagaa
     1561 cacgtaaccg ccgacgccct gaatcgcatt gaagccctgg gtcggcagaa tcttcaagag
     1621 atcggtgccg cgcttcttcc gcccgccctg cgacgcccgg tgcgcctccg caaagccgaa
     1681 cggctcgacg aaccaccgca cgtgatgccg cacgcctgcg gcttccttga cgttccgggc
     1741 catcgtgtac ttgaacgcct cgacagtggc cagcgaatcg gtcgcctggc cgccgaaccg
     1801 cccgacgatc ccttccgaga ccgccttgtg gtcgctgatg accagcgtgt cgtccttgat
     1861 gaagtagtag ctctgctcgg cgactttttc gccagctttc agcggctgcg tatagacggt
     1921 catcgtcacg ccgccgaacg cgtgcgccgt gcggaccgct ttgccttcct tctgattctt
     1981 atcgaccttg gccagcatct cgtcggcctt gtcgcgcttg cccgtgatat cgacgatgag
     2041 caccgtcgcg tgcgacatct tatctttcgg gtccggttgg atcagagcca gcgtcgattc
     2101 gcccgcgtac acgccttcca tatctttcca cttcagcccc agccgcttgc cggccttctc
     2161 gagcttcgcg ccgatctgct tgcgcaggtc gtcgatgaac ggcttcatga ccggatcggc
     2221 ggccatttcg cccaactgcg tctcgttgaa tttgttccgc acctcgtcga cgtcgtgcgt
     2281 cgagatgaag cccttcgtgg tggcgggcaa cagcgattcg ctcggctgca cggccaggcc
     2341 gcgtgtggcg aaggagatga cgagcgcaac ggacaacagg cgaacagacg agaaacgcat
     2401 cacttcgggg ctccttcgct ggattccgcc tgggcgagcc agtcggctgc aaacgacggg
     2461 ggaggatgag cgggcggtga tcggcgggtt gtaaagcttc tagtggtatc ggcagttcgg
     2521 cccgtgcaaa cgaacgaacc cgattatctc ccaatttggc ccgccacact acacctgtcc
     2581 attattcgga tggcgggccg gctggcaagg tggtcaagct gcggcgacct tgcggacgca
     2641 caccaacccg aagcgtaagc gagagaatga ctgggttcgt gtggactccc tcgcttacgc
     2701 ttcgggttac tgtctcgcta gcagccgata gctgcgggca ttgcgagcga tctcgtcttc
     2761 ttccaggcag cggctgtgga tggcgatggc ctcgatcgtt cccagccacg gcgattggcc
     2821 gctggcatcg gctccgagtg tcagcggccc gccgcgccac atcgagaacg aaccttggaa
     2881 cttgtcgctc tccgtcacct tctcgccatc gcgatagatg acgagtgagc cgtcgcggta
     2941 ggcgattgca atgtggctcg tttgcgtggg agtggccggc gccacccaga tcgtctcgcc
     3001 cacgtcgccc gcgtcggtca gaagctgaaa cccgagccgc tggccgtcct gcaaaagcgc
     3061 gaagttcgcc gcctggccat cgtcggccag cgcgacgatc cgtgccggcc cctcttgttc
     3121 ggctgtggtg gcggagaaga cgatctcgat ggtcagctcg ctcttgtcgc tgagcgatgc
     3181 cagcagatcg acgccggcgt cgtctgatcg tgcatgacct ccttggaatt cgacggcaga
     3241 agtgaattcg tttactgtcg tcgcgccgct caattccaaa ggtgtatctc gcaggctgct
     3301 cgtcatggga ttccgcgacg cgctcactgg cgcaaactcg gtcaggaaat cgaacggcgt
     3361 atagatatag gccacgtttc tgtgatcctc cggccacgcc agcggccgga cctgcaacag
     3421 ctcgctcgat gccagccccg tttcgttcgc gccgacggcg atcgattgct tgtccgccag
     3481 ccgatcgagc agcaccttgc cttcggtcac atccaagagc gtcgtcgtgg cgctgaccgt
     3541 cagccggaac tcggtgccga cgacggtggc gacggcgtgc ggcgtcgtca gcttcattgg
     3601 cgcgccggcg ccttgcggcg aaacgaccgc cgtgattgcg ccgacctcga tcgccacgcg
     3661 cttgccgccg gttgcgtcgt cgatgtcgag cgtcaccgtc gagccgccgg caatctgcac
     3721 ggtcgtcttg tcggcgtact cgatcgccag cgaagctccg tccggcgtca cgatctgttg
     3781 cccgctcagc accgcccggc ccacgccgat cgcggcgggc tggccggcga cttgcacgtc
     3841 tccctgcatc gcggtcactt tcgccagcgg ctgcggcgtc cgcggcagcc agcgggggac
     3901 gaagatcacc gcggcgatca gcacggtggc cgccagggcg atgtacttga cccacggcga
     3961 gctgcccgac caggtcgtcg gccgctcgaa ctcgctttcg gccagggccc gcaattcgga
     4021 cacctggtca tccatctcgg cttgcccgcg gtggaagtcg gcaatccgct ggccaacctg
     4081 cgcctggaaa atccgccgat cgaccgccag cttttgcgcc agcaggtcgt cgagcagcag
     4141 ctgctcgcgc agcgccgccg cccgcgccgg ctccgcccgg agcagcgcaa tcagctcgtc
     4201 cagctccgca gcgtccaagc tggagcggtc gtcgaggtag cggtggatga gggattcggg
     4261 ggtggacatg gatagcattt tcaaatgcgg agtgcgaaat tcggaatgcg gagtggcggc
     4321 aacagcttgc ggccttcact ccgcatttcg cactccgaat tccgcatttg cctcacgctt
     4381 cttccttcgc cagattcttc gtaatgcagt tcttcaggct cgcccggatg cggaacaggg
     4441 ccacgcggac cgcttcggcg gtcgagttca cccgctcggc gattgtgtgg ctcggcagct
     4501 cctgctcgta acgcagggcc aggagccgcc ggctttcgcc gggcagtttt tcgtagcagc
     4561 catccagggc ccggcggacc ggcccttccg agagggaaac ggcacattcc tgctcggccc
     4621 gcaggaaaat ctcgaccagg gcctcccgct gcaaccgctg ccgccgcccg gtgcggcgga
     4681 agtaattcag gcacaggttc cgggcgatcc ccttgagcca ctgcttgggc gtcacctccg
     4741 gcggcacgcg gtccatgaac cggtacagct ccagatagac gtcctgggcc acgtcatcca
     4801 cgtcgtgccg cggcaccccc ataccggcga tatacgcccg gatgtggggt tgcgtgtcgc
     4861 ggacaaccac gtcgaaatcc aagctcgcaa gtggctgatc gctcatctcc gccttccctt
     4921 tgctgcccaa actgagtggc ccccgccggc cgctttgtta cccgacccgc gattccgctg
     4981 gcaactcgta ggtcccgacc gcctgggcgg gaccggactc gcgagccagc ttctttggcg
     5041 cttttagttc cgccgcggcc gttggaacct acgcccgtgc gctgcgcaac gcgcaacttt
     5101 ccgctttttt tcgtgccgac ctcctccgcg caacttcgtt ataaactgca tcacaccaac
     5161 cacttgcgaa gcgcaacacc ccccacatga ggatgttgcg ctgttgcgcc ttgtcaatca
     5221 tttttcctcc atttttctgg cccgcacatc caatccatcg cccgatgacc gttccatttc
     5281 gcgcgggcaa gcgcaccgcg ctctgcccgt cttgggatcc cgcgggcgcc gtctctccgc
     5341 gcgcaccaac taaccatcct tatagatacg cacggaagtt catttatttc ttgattcccg
     5401 gattttcttt gaggaaccgc cgagaatgtc catcccggct gtgtagtttt cgccgggcag
     5461 gcccagatca caacaggcgc gtctaccgct gcaaatcgct ggaaagcgcc ttgcgcaatt
     5521 ctttggccag cgccgcgacg gccggatcgg agtcgtcttc acaaggcttc aactgcagcg
     5581 ccagcgactc ccgcctcttt gccgggaact gggtcacgtc gaggttcttg aacgattcgg
     5641 agattctggc cagcagcgcc ttgcgctggg cgggatcgtt accgaagaga acccgatagt
     5701 ttgctcgttg ttgatattca ggctcgtaag tcttctccca aaccagcttg tcgtttttgc
     5761 gatctgcgcg cccgtatcta tttcttccca gcacgaaact tgccaaaaaa gtgccggttt
     5821 tcgtctctgc gaagtccgcg ctgatgttaa cgtcatcgat gagccagggg gtcttcgatg
     5881 atgcgtaggt atgcgaggac ataccgcacg acgcgccctc ccaggtcatg ccaccgcatc
     5941 ctagtgctgt aacgcgttgc gtattcagaa acgcctccag cttctcgagc gggatctcag
     6001 ccggatagcc agccggtcgc cggacatccg gattgagaat gaaatggatc ggaatcccgt
     6061 cgatccgatc acgatcgacg cccatcacga cgcccggcgc ctgatcgccg ggctcgatcg
     6121 aggccgggtc atccgcgcgg accatcgaaa ccagggtcac ggcgacaacg acacagccga
     6181 tgaggaactt cgcccgtgac atggaagact cctgtgccag tgccgccgag aatctaccgc
     6241 gcagaactgg ctccgtttca caatatcaat cctgaagagc tgtttcaatt taaatcgtca
     6301 gccgcgcgga gaccttcggt cgagcgggtg ggccggtcgg cagaccgggc cacaacaggc
     6361 gtagcgtctc taaactctta tcccggcgcc tccttccctc cgagcgcgaa attgagccgg
     6421 gcgaggcagg tttgggtcca gggccagagg cccattttga ggaggctgat gaccagggcg
     6481 atggtcgtgg cggccaggcc ccaggcgagg aacgggaggc cgtcggcaag ctgcgtccgg
     6541 tccaggaggc cgtaaacctg ccgggccagg accagcaggt tcgccgcgag cgtgacgatg
     6601 gcggcggcca gcagcggcac ctccttctgc cgtcgccaga gcgcgaccga tgccaggagc
     6661 aacagcacca gatagaccgc cagcgacatt ggattgaccg ccggcaggat ccacgggtag
     6721 gcgactgccg cgaaggccgc cagcgccggc gcggcccgcc aggccagctc gcgcaagaat
     6781 cgggcgaatt catcgttgag gaccgcggcg accgcgacca ggccgatcag cggcgcatgc
     6841 cactgccaga accaggccgc ttcccagctt ccgccgccgg taaagcgaaa cgcgaacgcc
     6901 gccatcgcac caactgcgac cgctcgccac gtcgattgcc gctggatcgc cagaaccagc
     6961 agcgtgccgg cgaccgccgc cagaaccaac ggctgcgggc gggtgaggct ttcgaggtcg
     7021 agcgtctggc ggccgatcac cgctgcggcc aatcccgcga cgagcgcaaa tccctctgcc
     7081 gcgcgaacgt tccgcagcca tgcccagaga tagaagccga tgaggctcca gatcgcgagc
     7141 tgcgccggcg cggcgagtgt gtgcgtcagc cggccgagaa aggcaacttc gaccgggctg
     7201 cgtccggctc ccggaaaaac gagcagcatg ccggctgcag gcaagagcag ccccgcggcg
     7261 atcgccccgg cacgatggcg ggccttgccc atctccagca ccagggccga ccacgccagc
     7321 accagcggaa acaggaaata cggccggaaa taggcgtcct gcccctggac cggctcgaac
     7381 gagatcgtca gccaccagct ccggatcgca aagccgacgg tgagaaacac gaacagcgac
     7441 cagggataga agggccagat ccagggcgtg ccgctggcgg gctcgtgccg ccgccgcgtg
     7501 gctgccgcgg ggaggagcgt gagcaggctg agtgcggcca gcaccggaaa gccgagcacc
     7561 gcccacggcc gggccgccgc taggccgaat tgattgatcc acacgaggac caccggccag
     7621 ccgaagagca gggccagcat caggtaatat ggccggcgat accgcggagc gaggtggatc
     7681 tgcagcccgg ccagcagcgt ctggctgagg ccgatcgaaa acgtcagccc cgccgccagc
     7741 accagcgacc cggccagcgg ctgcgtgagg agcagcacgt cgaggctcga cgagagcatg
     7801 aagaacatca gcacgatcac cagcaggatg gtgcgggcgt cgtcccacac cttgccatag
     7861 cggatgatga ccgccgcgat cgccgccaac agcagcgtga acccggcgag gccgaccgcc
     7921 aacaggccgc tcgtcgccag gccgggctct ttgcccagcc cctgctgcag gccgacgagg
     7981 atcagcagcg tgccgatcag gtagaagggg ttctgcgtgt acagcagccg taccagcgac
     8041 tgcaccgact cggcagcagc cccggcgccc gaagcgtgag cgaactctgg ttctttggtc
     8101 atggcgagcg ctcctttccc aagcgaagaa gtagaaagtt tgccgaagcg aggcgaacaa
     8161 aagctggcga agaaccaaga accaaagaac caagaaccga agaaccaagt cggttcttgg
     8221 atcttgcttc ttggttcttt ccccagcggt gaagcggtca cgccaaccgc ccgcgcatca
     8281 ccaggaccga aaagaacgaa caggataaga ccgaatcgca gtccccagcg acgaaccgcg
     8341 cgctgccagt ccagaccgcc tagccgcact gcatgcagaa tgtttgtcga atgaccacct
     8401 cccctcaaga aaagccgtgc aaggggaagt caacaccaac atctttgaat ctagcgcagc
     8461 ggcagggcga aagtcaaatc gaattgcgta atccgccgtg gctgagcagt gcccagccac
     8521 ggcccatgat gatcgccgct agcccgccag ggcggcggct gtgggaacgt tggcaccggc
     8581 agcctgtgca gccggcagct cgacctgcgc ctcgcggcgc tccagctccg cggccagctc
     8641 ggccagcacg atctggggcg tgagcgagcc gtcgtcccag cgccgcatgg tgcgcgaagc
     8701 ggccatgacg aggcggtcgt ccacgtccca cttccacagg tcttcgctgc tggtccagcc
     8761 caggagctgg ttcagcaggc atccccggtt gccttcgggc gcggtgcgat cgacgaacat
     8821 gccccggacg acccggttgg tgcccagcgc atcccgcagc cggccaagct gctcgctcga
     8881 tgccgcgcca aaaattcccg ccagatcggc cttggggcag gcctggcggc tgccgtccag
     8941 ctcggcggtg ggccagtagg acaggcccag ctccagccgg cggcgaagtt cggtgcgaat
     9001 ggacataact cacctccatc aagtgggaaa ggaaaggaac tctattgctt agggggcggc
     9061 gctggcgacc accgcgcggc cgaccagcgc ctgccaatcg cgctcgggcc gaacccgcag
     9121 gttggtggcc caggcgcctg cgatgacctg ggcgtcgatc tgcagatcga cgcacaaggc
     9181 caacagcgcg ttgatatcct tgggaaagca cgagcccgac aagccgcggc ggccgtcagg
     9241 tccgggcacg tcccagtggc tggtgggcag gcgcttgtcg agctgcgcca ggttgcggac
     9301 cgcggtgtag tccagctcca gccgctgggc gatgtcgtag aactcgttgg ccagcgaaac
     9361 ttgcacggcc agcgtgacat tccggacgta cttgacgagc tcgccgattt
//
LOCUS       DASUGB010000431         8866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_466202, whole genome shotgun sequence.
ACCESSION   DASUGB010000431 DASUGB010000000
VERSION     DASUGB010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466202"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFV87_08375"
     CDS             <1..646
                     /locus_tag="VFV87_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103810.1"
                     /translation="TKVSVFAPWKDGGYAVVDVPEAIWHSVPEGRALLYLAHTHVPTI
                     WDKQGVTLEPLEWKPTKTGWEVERTLPNKVAFGSRVTPQRDHVAMEMWLTNGTTESLR
                     GLTVQNCIMLKGLAGFEQQTNDNKLLQKPYAACHNPDKSRWIITAWEPCQRTWANPPC
                     PCLHSDPQFPDCEPDRTVHLRGWLSFYEGKDIDAEIKRIDGLGWKTAPLTYSER"
     gene            709..1707
                     /locus_tag="VFV87_08380"
     CDS             709..1707
                     /locus_tag="VFV87_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5103811.1"
                     /translation="MDDSAGSLMAGQVRRLRQAATESLAGYDIEATRVRLLAHHRQTT
                     FQVISGRDGERYLLRLHRAARRPPAAVQFELWWLAELRDKTKIVAPLAVTAKDGRSVL
                     PVRGMGIPDDLYCSLLRWVPGRRYFRRTGPGVWVLHEVGRHMAVMHDLAQRCNLPRTP
                     PPVPIWNAERLFGVTEQEKTAERQQLTLHQRKLFQRAARQAAEVMEHLGVGRQEFGLI
                     HADLIQSNYLVHRGAVHLIDFAELGLGHYLYDLAVTIYGLWGLDPQRNQRQALLEGYR
                     LVRELPMRQVDLLDRLVAGRAVVQGRFVMSSEHAADREIAPRYVRQALDCLTAYSG"
     gene            complement(1737..3107)
                     /locus_tag="VFV87_08385"
     CDS             complement(1737..3107)
                     /locus_tag="VFV87_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5103812.1"
                     /translation="MTRLPPGPRDSLFGLRLGGRFRRNPLRFLTNLLRDYGDLAMFRL
                     GPFRCCLVNHPDLIREVLITQRPLFPKLERHLRTIRSFSGNNIFVSDGERWPRQRQLL
                     QPAFQPRMFERYAQATVKHAQRLVAEWSDGAELDLVQAMARLAVVSIGEALFQVDLSD
                     RVEVCRRALNIHSETLRDEFRALFVLPDWLPTAAKRRKKWAVRTLCELIRETFRERRA
                     AGGGGDDLLSILLAAVNDPRSESRMTQQQALDEATILFVAGQDDITAALSWCWCLLAQ
                     NRECEERFRAELATQLGGRLPGYADVARLPFTEMILKETLRLYPPTWTLVPRCAAREV
                     TLGGFRIPRGTWLFISPYATHRDPRFFPVPERFDPDRFSPERQPAAQFAYIPFGGGPR
                     ICIGNHFSFTLLTMALATIAQRFRMTLCDDPAAIVPDPGLALRPRGGLRVRLEAIASQ
                     ASTAIA"
     gene            3336..4421
                     /locus_tag="VFV87_08390"
     CDS             3336..4421
                     /locus_tag="VFV87_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX5103813.1"
                     /translation="MVLAQARQSSSAALRAARGLRRPGKFAGQSPAADSAPATAAERL
                     ELVRAHGDFSLAYTTAVQSGLSYFGDNRGYLAHDSRLGHTFVLADPVAAPPDRERLVR
                     QFAAMHRRLAFCQISRATAQIVQELGFYVNEMGVDSIVPLAGYDFRGREKKWLRTAES
                     WTSRRGYTTREDRAQHVGLAQINAVSLAWRATRTVKFKEVRFLNRPLVASDEPGTRRF
                     FFFGPGERLLAFVFFDPLYRDGRLVGYAACSKRRHPEAPVYAEQAIMKHAIEVFQRED
                     CQELRLGLSPLAWIEDREFRASWLLRTLFRGGFSSRLINRRIYNVQGHAEYKRRYRGR
                     EEKLYYATRTPLNLLQLAALVKTCKIL"
     gene            complement(4487..4957)
                     /locus_tag="VFV87_08395"
     CDS             complement(4487..4957)
                     /locus_tag="VFV87_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103814.1"
                     /translation="MRPSVITSCLLAACLPLLAGCGSSHSSAGQLEASELIELLAEQE
                     LKYDSQAYVEVDLGEFRVTHALAGGEGQILVRFHLFGIVPEERAAKLEQTLPHFQNRL
                     RDAVIQLVQKTETEQFGDPGLAFFRAEVIAAVNRILQDRLLKDVAFSDFSVEPG"
     gene            complement(5151..7721)
                     /locus_tag="VFV87_08400"
     CDS             complement(5151..7721)
                     /locus_tag="VFV87_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX5103815.1"
                     /translation="MYERFTDRARKVMQLANQEAQRFNHEYIGTEHILLGLVKEGSGV
                     AANVLKNLDVDLRKIRLEVEKLVQSGPEMVTMGKLPQTPRAKKVIEYSMEEARNLNHN
                     YVGTEHILLGLLREQEGVAAQVLMNLGLKLEEVREEVLNLLGHGIEGEGSERGGREGA
                     GVSGSSAEGGKESSGRSGKSKTPALDSFGRDLTELARQNKLDPVIGREREIERAIQIL
                     CRRQKNNPVLLGEAGVGKTAIVEGFAQRVVSGNVPEILAEKRIVVLDLAMMVAGTKYR
                     GQFEERIKAVMNEVRRARNTILFIDELHTLVGAGGAEGAIDAANVLKPALSRGEIQCI
                     GATTLDEYRKYIEKDSALARRFQEIIVDPTTKEETIAILKGLREKYEEHHRVQITDDA
                     VEAAVELSERYITARCLPDKAIDVIDEAGARVRLRAMTRPPDLKEIDEDIERLNKEKE
                     EAVANQDFEKAASLRDSADKLRKKKENITKEWREKSRATDGVVDEEVIAEVVSKMTGI
                     PLTRMSTEDSLRLMKMEDELHKKVISQDQAVKAVAKAVRRSRSGLKDPRRPTGCFLFA
                     GPTGVGKTLLAKALAEFMFGDADALVQIDMSEYMEKHNVSRLIGAPPGYVGYEEGGQL
                     TEKIRRRPYAVVLLDEIEKAHPDVFNTLLQVMEEGRLTDSFGRRVDFRNTIMIMTTNA
                     GAEAIKNESAFGFRKEDGDSSYEGMKERVQERIERVFRPEFLNRLDDTIVFRHLTVED
                     LKEVIDLELKKVRERLMERGLKLELTDSAKEFLIKKGSNTDFGARPLRRAVENFVEDP
                     LSEELLKGEFQGKDTIVVDAIKDDEDKPRRLDFKGSITNHQAAAVSQPEPVAAGSEEK
                     "
     gene            complement(7896..8492)
                     /locus_tag="VFV87_08405"
     CDS             complement(7896..8492)
                     /locus_tag="VFV87_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103816.1"
                     /translation="MRAPGNVVAWIACLWPGLLQAWRLGSLWGLGVAMAFAAALNAAL
                     IATLVWPRWPIASLPAGTAATAAWAGVLGLWILGLRWTSRNWSQLCPPRPQADPQLDD
                     WFREAQHEYLKGHWLEAETLVSRILARQPGDCETRLLLASIQRRRGSRDQAQQTLSEL
                     QPVASKWQWEIEAELTQLNELEAEKNETLTVDAMPRAA"
BASE COUNT         1495 a         2919 c         2775 g         1677 t
ORIGIN      
        1 gacgaaggtc agcgtgtttg ctccttggaa ggacggcggc tacgcagttg tcgatgttcc
       61 ggaggcgatc tggcattccg tccccgaggg ccgggcactc ctttacctgg cccacaccca
      121 cgtgccgacg atctgggaca agcagggggt caccctggag ccgctcgaat ggaagccgac
      181 gaagaccggc tgggaggtcg agcgcacgct gcccaacaag gtcgcgttcg gcagccgggt
      241 cacgccccag cgggatcacg tcgcgatgga gatgtggctc accaacggca cgactgaatc
      301 gctccgcggc ctgaccgtcc agaactgcat catgctcaag gggctcgccg gcttcgaaca
      361 gcagaccaac gacaacaagc tcctgcaaaa gccctacgcc gcctgccaca acccggacaa
      421 gtcgcgctgg atcattaccg cctgggagcc ctgccagcgg acctgggcca acccgccctg
      481 cccttgcctc cacagcgatc cgcaattccc ggattgcgaa cccgaccgga ccgtccacct
      541 ccgcggctgg ctgtcgttct acgagggcaa agatatcgac gccgaaatca agcgcatcga
      601 cggcctcggc tggaaaactg cacctctcac ctattccgag cgatagcccg attgcggaca
      661 gcgccggtag tgccccggcc gctggctgtt ccttcgtggc tgacttcgat ggacgattcc
      721 gcggggagtt tgatggccgg acaagtgagg cgattgcgcc aggcggcgac ggaatcgctc
      781 gccggctacg atatcgaggc gacacgcgtg cgcctgttgg cccaccatcg gcagacgacg
      841 tttcaagtca ttagcggccg agacggcgag cggtacttgc tccgcttgca ccgtgcggcg
      901 cggcggccac cggctgccgt tcaattcgaa ttgtggtggc tcgcagaatt gcgcgacaag
      961 acgaagatcg ttgcaccgct ggcagttaca gcgaaagacg gtcggtccgt gctgcctgtc
     1021 cgcggtatgg ggattccaga cgatctatat tgttcgctct tgcgctgggt gccagggcgc
     1081 cgatactttc ggcgaacggg ccccggggtg tgggtcctgc acgaagtcgg ccggcatatg
     1141 gcggtcatgc acgacctggc ccagcgatgc aatttgccgc ggacgccgcc accggttccg
     1201 atctggaacg cggagcgcct gtttggtgta accgagcaag agaaaactgc cgagcgccag
     1261 cagctaacgt tacatcagcg aaagcttttc cagcgcgccg cgcggcaggc ggcggaggtg
     1321 atggagcatc tcggtgtcgg tcgccaggaa ttcggtctca ttcatgccga cctgattcag
     1381 tcgaactatc tggttcaccg cggcgccgtc catctcatcg acttcgccga gctcgggctg
     1441 ggacattacc tctatgatct ggctgtcacg atttacggcc tgtggggact cgacccgcag
     1501 cggaatcagc ggcaggcatt gcttgagggc taccgcctgg tgcgcgaact gccaatgcgg
     1561 caagtggacc tgctggatcg cctggtggcg gggcgagccg tcgttcaggg acgctttgtg
     1621 atgagctccg aacatgcggc ggatcgcgaa atcgcgccac gttatgtgcg gcaggcgctg
     1681 gactgtctga cggcgtatag cggttgaaat tcaccagaat cctcggccgc ccgatcttag
     1741 gcgattgcgg tcgatgcttg ggaggcgatc gcctccagcc gcacgcgcag gccgccgcgg
     1801 gggcgcaggg ccagcccggg atcgggcacg atcgcggccg gatcgtcgca gagcgtcatg
     1861 cgaaaccgct gggcgatcgt ggccagggcc atcgtcagca gcgtgaacga aaagtggttg
     1921 ccgatgcaga tccgcggccc gccgccaaag ggaatgtatg cgaactgcgc cgccggctgc
     1981 cgctcgggcg agaaacgatc ggggtcgaag cgctccggta cgggaaaaaa ccgcggatcg
     2041 cggtgcgtgg cataggggct gatgaacaac caggtgcctc gcggaatccg gaagccgccg
     2101 agcgttacct cccgcgccgc gcagcgcgga accagcgtcc acgtgggcgg atacagccgc
     2161 agcgtttcct tgaggatcat ttccgtgaac ggcagccggg cgacatcggc ataaccgggc
     2221 aacctgccgc cgagttgcgt ggcaagctcc gctcggaagc gctcctcgca ctcccggttt
     2281 tgagccagca ggcaccagca ccagctcaat gccgcggtga tgtcgtcctg cccggcgacg
     2341 aacagaatcg tcgcttcgtc gagcgcctgc tgctgggtca tccggctttc gcttcgagga
     2401 tcgttcaccg ccgccagcag gatcgacagc agatcgtcgc cgccgccccc ggcagcgcgc
     2461 cgctcgcgga aggtctccct gatcagctcg cacaaagtgc gtacagccca ctttttccgc
     2521 cgcttggcag ccgtggggag ccagtcgggc aggacgaaga gggcccgaaa ctcgtcgcgc
     2581 agcgtctccg aatgaatatt gagcgcgcgg cggcaaactt ccacacggtc cgacaggtcc
     2641 acttggaaga gcgcctcgcc aatgctgact actgccagtc gggccatcgc ttgaacaagg
     2701 tccagttcgg ccccatccga ccattcggca accagccgct gcgcgtgttt caccgtcgcc
     2761 tgggcgtagc gctcgaacat ccgtggctga aacgcgggtt gcaaaagctg gcgctgcctg
     2821 ggccaccgct cgccgtcgct gacgaagatg ttgttgcccg aaaagctgcg gatcgtgcgc
     2881 aggtgccgct cgagcttcgg gaacagcggc cgctgcgtga tcagcacctc gcggatcagg
     2941 tccggatggt tgaccaggca gcagcggaac ggccccaggc ggaacatcgc caggtcgcca
     3001 tagtcgcgca acaggttggt gaggaaccgc agcggattgc ggcggaaccg gccccccagc
     3061 cgcagcccga agagcgaatc gcgaggtccg gggggaaggc gagtcatagg cggaacgggc
     3121 ggctgaaatc gcggcatccg cacaaccact gccggcgggc tcaccgctac ggataacaag
     3181 accgcgggcg aaccattcgc gaacgccccg gcgggaaacc ggcacatctt acccaaacga
     3241 ttgccgccgg cgcgcgtgat agcgggcggc cgaacttccg atcaatgcgt taggcggcgg
     3301 cagctagcag ccgcgagcac cttggaggca gtggaatggt cctggcccag gcccggcaat
     3361 cgagcagtgc ggcgctgcgc gcggcgcgag gtcttcgccg cccaggcaaa ttcgccggcc
     3421 aatcgcccgc cgccgattcc gcgccggcca ccgcggccga gcggctggaa ctggttcgcg
     3481 cacatggcga cttctcgctg gcgtacacga ccgcggtgca gtccgggctg agctacttcg
     3541 gcgacaaccg cggctacctc gcccacgaca gccgcctggg acacacgttc gttctggccg
     3601 atccggtcgc cgcaccgccg gatcgcgagc gcctcgtccg gcagttcgcc gcgatgcatc
     3661 gccggctggc gttttgccag atttcgcggg ccacggccca gatcgtgcag gagctgggct
     3721 tctacgtcaa cgagatgggg gtcgattcga ttgtcccgct cgcgggttac gactttcgcg
     3781 gccgggagaa gaaatggctg cggacggccg agtcgtggac cagccgccgc ggatacacca
     3841 cgcgcgagga ccgggcgcag cacgtcgggc tcgcgcagat caacgcggtt tcgctggcct
     3901 ggcgcgcgac gcggacagtg aaatttaagg aagtccgctt tctgaaccgg cccctggtcg
     3961 cgtccgacga gcccggcacg cggcggttct tcttcttcgg ccctggcgag cggctgctgg
     4021 cgtttgtatt cttcgatccg ctctatcgcg acgggcggct cgtcggctac gcggcgtgca
     4081 gcaagcggcg tcatcccgag gcgccggtct atgccgagca ggcgatcatg aagcacgcga
     4141 tcgaggtctt ccagcgcgag gactgccagg agctgcgtct ggggctcagc ccgctggcct
     4201 ggatcgaaga ccgcgagttt cgtgcgagct ggctgctgcg cacgctattc cgcggcggct
     4261 tctcctcgcg gctgatcaat cgccgcatct acaacgtgca ggggcacgcc gaatacaaac
     4321 gccgttaccg cggccgcgaa gaaaagctct actacgccac ccgcacgccc ctcaacctcc
     4381 tccagctcgc cgctttggtg aagacctgca agatcctcta acccagccgc cgcagcggaa
     4441 tcgaagcaga gggaaagccc gattcgtcac cgtgggagcc tggatttcag cccggctcga
     4501 cggagaagtc gctgaaggcg acgtctttga gcagccggtc ttgaaggatg cggttgacgg
     4561 cggcgataac ttcggcgcgg aagaatgcca ggccaggatc gccgaattgc tcggtttcgg
     4621 tcttctgcac cagttggatt actgcgtccc gcaggcggtt ctgaaagtgc ggcagcgttt
     4681 gctcgagttt ggccgcccgc tcttccggga cgatgccaaa caggtggaac cgcaccagga
     4741 tctgtccctc gccgccggcc agtgcgtgcg ttacgcgaaa ctcgcccaga tcgacctcga
     4801 catacgcctg cgaatcgtac ttcagctcct gctcggccaa cagctcgatc agttcgctcg
     4861 cctccagttg cccagcagac gaatgcgaac tcccacagcc ggccaggagc ggcaggcagg
     4921 cggcgaggag gcaggaagtg atgacggatg ggcgcatggg aaacggatgc cagattgtcg
     4981 tcgctcgctc cgcgaacgaa cgccctttcg cggagcgaaa ggcgacaatg ttcgaggcgg
     5041 tgaccccttc cggcaagcct agctttcgcg gccagcgcgc gaggcaggcc gccgattgcg
     5101 tcggcggccc gcttgcttgc taaacgttcc gattgtgcgg accgtgcggg ctacttctcc
     5161 tcgcttcccg ctgccaccgg ctccggctgt gaaacggcgg cggcctggtg gttggtgatc
     5221 gagcctttga agtcgagccg ccgcggctta tcttcgtcgt ccttgatggc atcgacgacg
     5281 atcgtgtcct tgccctggaa ttcgcccttg agcagctcct cgctgagggg gtcctcgacg
     5341 aagttctcga ccgcccggcg cagcggacgg gcgccaaaat cggtgttcga gcccttcttg
     5401 atcaagaact ccttcgccga atcggtcagc tccagcttga gcccgcgctc catcagacgc
     5461 tcgcggacct tcttcagctc caggtcgatc acttccttga ggtcttcgac cgtcaggtgg
     5521 cggaacacga tcgtgtcgtc caggcggttg agaaactccg gccggaagac ccgttcgatg
     5581 cgttcctgca cgcgctcctt catcccctcg tacgacgagt cgccgtcctc cttgcggaag
     5641 ccgaaggccg attcgttctt gatcgcctcg gcgccggcgt tggtcgtcat gatcatgatt
     5701 gtgttacgga aatcgacgcg gcggccgaag ctgtcggtca gccgcccttc ttccatcacc
     5761 tggaggagcg tgttgaacac gtccgggtgg gccttctcga tttcatcgag cagcacgacc
     5821 gcgtacggcc ggcggcggat cttctccgtg agctggcccc cctcttcgta gccgacgtag
     5881 cccggcggag cgccgatcag gcggctgacg ttgtgcttct ccatgtactc gctcatgtcg
     5941 atctgcacga gggcatcggc atctccgaac atgaattcgg ccagggcctt ggcgagcagc
     6001 gtcttgccca cgcccgtcgg cccggcaaac aggaagcagc cggtcggccg ccgcgggtct
     6061 ttcagaccgc tgcggctgcg gcggacggcc ttcgccaccg ccttcaccgc ctggtcctgg
     6121 ctgatgacct tcttgtgcag ctcgtcctcc atcttcatca ggcggaggct gtcctcggtg
     6181 ctcatccgcg tgagcgggat gccggtcatc ttgctgacga cctcggcaat cacctcctca
     6241 tcgacgacgc cgtcggtcgc ccgcgatttc tcgcgccact ctttggtgat gttttccttc
     6301 ttcttgcgaa gcttgtcggc cgaatcgcga agcgaagccg ccttctcgaa atcctggttg
     6361 gcgaccgcct cttccttctc cttgttcagc cgctcgatgt cctcgtcgat ctccttcagg
     6421 tccggcggcc gggtcatcgc ccgcaggcgg acgcgggccc ccgcttcgtc gattacgtcg
     6481 atcgccttgt ccggcaggca gcgagccgtg atgtaccgct cggaaagctc gacagccgct
     6541 tcaaccgcgt cgtcggtgat ctgcacgcgg tgatgctctt cgtacttctc gcgcagcccc
     6601 ttgaggatcg cgatcgtctc ttccttcgtg gtcggatcga cgatgatctc ctggaaccgg
     6661 cgtgccagag ccgaatcctt ctcgatgtat ttgcggtatt cgtcgagcgt cgtcgcgccg
     6721 atgcactgga tttcgccgcg cgagagggcc ggcttcagca cgttggccgc gtcgatggcc
     6781 ccttccgcgc cgccggcgcc gaccagggtg tgcaattcgt cgatgaacag aatcgtgttg
     6841 cgggcccggc ggacttcgtt catcaccgcc ttgatccgct cttcgaactg gccgcggtac
     6901 ttcgtgccgg cgaccatcat ggccagatcg agcacgacga tccgcttttc cgccagaatt
     6961 tccggcacgt tgccgctgac gacccgctgg gcgaagcctt cgacgatcgc cgtcttgccc
     7021 acgcccgctt cgcccagcag cacggggttg ttcttctgcc ggcggcagag gatctggatt
     7081 gcccgctcga tctcgcgctc gcggccgatg accgggtcga gcttgttctg gcgggccagc
     7141 tcggtcaggt cgcggccgaa gctgtccaaa gccggcgttt tgctcttgcc gctgcggccg
     7201 gagctttcct tgcctccttc ggccgaggaa ccgctgaccc ccgcgccttc acggccaccc
     7261 cgctcggagc cttcgccctc gatgccgtgg cccaaaaggt tcagcacctc ctcgcggacc
     7321 tcttccagct tcaggcccag gttcatcagc acctgcgccg ccacgccttc ttgctctcgg
     7381 agcaggccca gcaaaatgtg ctcggtgccg acgtagttgt ggttcaggtt gcgggcctct
     7441 tccatcgagt actcgatgac cttcttggcc cgcggcgtct gcggcagctt gcccatcgtg
     7501 accatctcgg ggccgctctg gacgagcttc tcgacctcca agcggatctt ccgcagatcg
     7561 acgtcgaggt tcttcagcac gttggccgcc acgcccgagc cttccttcac cagccccagc
     7621 aaaatgtgct cggtgccgat gtattcgtgg ttgaaccgct gagcttcctg gttggccagt
     7681 tgcatcactt tgcgggcgcg gtcggtaaag cgttcgtaca tggtgtttcc ccctgggtca
     7741 gtttcccgtg gcggctactt tcagtgaatt cgtgagcttc ctgcgggcag gcacttcgga
     7801 atcgttcgtg gttacgtcag tattgtttcg ctctcggtgt cattcgccaa gtggcggctc
     7861 ttgcgtagga cgggtttccc aagtgcgtcc ttgcctcacg ccgccctggg cattgcatcg
     7921 acagtcagtg tttcgttctt ctccgcttcc agctcgttca gttgcgttag ctccgcctca
     7981 atctcccact gccacttgct ggcgaccggt tgtaattcgc tcaaggtttg ctgggcttga
     8041 tcccgcgagc cccttcgccg ctgaatcgat gccagcaaga gccgcgtttc gcaatcgccc
     8101 ggctggcggg cgaggattcg cgagaccagt gtctcggctt cgagccaatg tcctttcagg
     8161 tattcgtgtt gcgcttcgcg aaaccagtcg tcgagctgcg gatcggcttg cggcctcggc
     8221 gggcaaagct gcgaccagtt tcggctggtc cacctgagcc cgagaatcca caaacctagc
     8281 acgccggccc aggctgccgt ggccgccgtg cccgcgggca ggctggcaat cggccaccgg
     8341 ggccaaacca gcgtcgcaat cagggctgca ttgagcgccg cggcaaacgc catcgccacc
     8401 cccagccccc acagggagcc gagccgccag gcctgcaata atccaggcca caggcaggcg
     8461 atccaagcca cgacgttccc tggcgcacgc atgcaatcca aactccaact tccgtgccga
     8521 ccaagcgccg cgttccgccg cggcgcaaaa ggctcgaaat tctacccctc gaatagtgct
     8581 ctcgcaaggc aagttcaagc gtggcaaaag gttacgtgaa attgtgcggc gcatgccgtc
     8641 cgtgcagctg gtcgtcttgc ccaaattgcc gcggagccaa gtgggcggtt attcattcac
     8701 gcgcgagtaa aattcgtttc cgctggatgg tgaatgtaag ttattgtaaa caaacgcctt
     8761 gcgccgaatt attcgcttcg ccgattatgc aaattattca caacatgcat aatcaggctt
     8821 cgggaccgcg aattgggacc acgctcacgg gtcgattcgc ctgact
//
LOCUS       DASUGB010000432         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_603695, whole genome shotgun sequence.
ACCESSION   DASUGB010000432 DASUGB010000000
VERSION     DASUGB010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603695"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1216
                     /locus_tag="VFV87_08410"
     CDS             152..1216
                     /locus_tag="VFV87_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5103817.1"
                     /translation="MKLKPLVSNALKVAVSAAMIALLVYQVRQDTQFYELAAKPKDWA
                     ILVWALPLCLAAVTLTILRWHLLVRTLGLQFQVREALRAGFLAYAFNLLPVGLVAGDS
                     LKAVMLIHRQPRRKTEAVTTVLIDRVIGLYALLLLAAAASLFLPPEQLERLEPMHRTT
                     ILTLCGTVRALAVGSTLGLIVMLIPAVTRSRLWDGLEHTPLIGGILHKLVGAMRTYRS
                     RPDRLLIAVALSIGIHLLYVTAVAFIAQGIGIAPSERPPVRSIFVIVPPSMIAGALPI
                     GVYEITITLLFRAISPPGAPPNTGLLVALCYRVIQILIGSIGVVYWLTSRGEVKELIH
                     EAEEQPPEELLDEPATATPA"
     gene            1391..>2383
                     /gene="metG"
                     /locus_tag="VFV87_08415"
     CDS             1391..>2383
                     /gene="metG"
                     /locus_tag="VFV87_08415"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5103818.1"
                     /translation="MARRILVTSALPYANGPIHIGHLVEYIQTDIWVRFQKLAGNRCV
                     YLCADDTHGTAIMISAKKAGVSEEQFIARMNAEHQRDFAGFQIAFDNYGSTHSPENRE
                     LCGQFWKSLRAAGLIVEKDVRQLFDPVAGTFLADRFVKGTCPKCKSPDQYGDSCDKCQ
                     STYSPADLIDPVSTLSGAKPELRSAKHLFVELEKLHGFLAEWTQSGKHLQDEVANYLQ
                     GHFLGEPLRDWDISRPSPYFGFEIPDSPGNYWYVWFDAPIGYIASTCQWCQRTGEKLD
                     DWWKSPACEVHHFIGKDITYFHTLFWPGMLKTAGFSLPTKVHIHGFLTVGGEKMS"
BASE COUNT          412 a          733 c          750 g          488 t
ORIGIN      
        1 ccgggtggat agtaggtaat atcggtcggt gaatgaccga atctctctcc ctcggcccgc
       61 ctgccggtga acctatcaat ggccccccgg agtgtcaagg acgctcgcag tcggcgtggg
      121 gttcggagta tcgtcgttag ctcgatccgt cgtgaaactt aaaccccttg tctcgaacgc
      181 cctgaaggtc gccgtgtcgg cggcgatgat cgcgctattg gtctaccagg ttcggcagga
      241 tacgcagttc tacgagctgg cggctaagcc caaggattgg gcgattctgg tctgggcgct
      301 gccgctgtgc ctggcggccg tgacgttgac catcctccgc tggcacctgc tggtgcggac
      361 attgggcttg cagttccagg tgcgcgaggc gctgcgggca gggttcctgg cctatgcgtt
      421 caatttgctg cccgtcgggc tggtggccgg cgattcgctc aaggccgtga tgctcatcca
      481 tcgccagccg cggcgaaaga cagaagctgt caccacggtg ctcatcgatc gggtgattgg
      541 cttgtacgcg ctgctattgt tggcggcggc ggcttcgctg tttctgccgc cggagcagct
      601 cgaacgcctt gaaccgatgc atcggacgac cattctcacc ctctgcggca cggtgcgagc
      661 gctggccgtc ggcagcacgc tggggttgat tgtcatgctc attccagcgg tgacgcggtc
      721 gcgcctgtgg gacggcctgg agcacacgcc gctcatcggc ggcatcctgc acaagctggt
      781 aggagcgatg cggacctatc gcagccggcc ggatcgtttg ctgatcgcgg tggcgctgag
      841 catcggaatt catttgctgt atgtcacggc ggtggcgttc atcgcgcaag ggattggcat
      901 cgcgccgagc gagcgcccgc cggtgcggtc gatcttcgtg atcgtgccgc cgtcgatgat
      961 cgcgggcgcc ctgccgatcg gcgtgtatga aatcacgatc acgctgctct tccgggccat
     1021 ctcgccgccc ggtgcgccgc cgaatacggg cctcctggtc gctctctgct atcgcgtgat
     1081 tcagatcctg atcgggtcaa tcggcgtcgt ctattggctc accagccggg gcgaagtgaa
     1141 agagctgatc catgaagccg aggagcagcc gccggaggaa ctgctcgacg agccggcaac
     1201 agcgacgccg gcctaaccgc tcgcgcctgc gtgagggcac gcggtgcccg tcgggcggcg
     1261 tatcggcagc acggccgcgt gaacctcgat ctcatgtcgc gtctccctgg aaacacggcg
     1321 cttcgctaca atcacgcctt aacccattgc tgccgctgga tttatcgctt tcgctatctt
     1381 gagagtccga atggctcgcc gcatcctcgt cacgtccgcc ctgccgtacg ccaatgggcc
     1441 gattcacatc ggccatctgg tcgagtacat ccagaccgat atctgggtgc ggtttcagaa
     1501 gctcgccggc aaccgctgcg tgtatctctg cgccgacgac acgcacggca cggcgatcat
     1561 gatttcggcg aaaaaggcgg gggtcagcga agagcagttc atcgcccgca tgaacgccga
     1621 gcaccagcgc gacttcgccg gctttcagat cgcgttcgac aattacggca gcacgcacag
     1681 ccccgaaaac cgcgagctgt gcgggcaatt ctggaaatcg ctccgcgcgg ccggtctgat
     1741 cgtggagaaa gatgtccgtc agctcttcga tccggtcgcc gggacgtttc tggccgatcg
     1801 gtttgtgaag gggacgtgcc ccaagtgcaa gtcgccggac cagtacggcg attcgtgcga
     1861 caagtgccaa tcgacctact cgcctgccga cctcatcgac cccgtcagca cgctcagcgg
     1921 agccaaaccg gaactacgct cggccaagca cttgtttgtg gagttggaga agcttcacgg
     1981 tttcctggcg gagtggacgc aatcgggcaa acacttacag gacgaagtcg ccaactactt
     2041 gcagggccat ttcctcggcg agcctttgcg cgactgggat atcagccgcc cgtctccgta
     2101 tttcggattt gagattcccg attcgccagg gaattactgg tacgtctggt tcgatgcgcc
     2161 gattggctac atcgccagca cttgccagtg gtgccagcgg acgggcgaaa agctcgacga
     2221 ttggtggaag tcgcccgctt gcgaggtgca tcacttcatt ggcaaggaca ttacgtattt
     2281 ccacacactc ttctggcccg gcatgctcaa gacggccggc ttcagcctgc cgacgaaggt
     2341 tcacattcac gggtttctta ccgtgggcgg cgagaagatg agc
//
LOCUS       DASUGB010000433         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1716798, whole genome shotgun sequence.
ACCESSION   DASUGB010000433 DASUGB010000000
VERSION     DASUGB010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716798"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFV87_08420"
     CDS             complement(<1..501)
                     /locus_tag="VFV87_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5103819.1"
                     /translation="MVVFIGSSASSGTPGSNVAEREVVITGVGIVSPIGVGREAVWNA
                     IESRQSGVRPIEQLASAGWLAPFGGEVTDFDPKELVQPRKSLKVMSRDIQLASAAAEL
                     AWQDSCLSQATLDADRFGVVGGAGVMYCDLEELRGPFLEWIKADDFDVHRWSRDAMGE
                     LYPLWML"
     gene            complement(991..1413)
                     /locus_tag="VFV87_08425"
     CDS             complement(991..1413)
                     /locus_tag="VFV87_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5103820.1"
                     /translation="MKLSAKTEYACLAMLQLAAEYPKGEPLQIRRIADVHGIPSRFLV
                     QILLQLKGASLVVSTRGAAGGYRLARSPSEITIADVIEVMEGDERPATNAGKNTPLVG
                     SLLHLCRELDEIQRDRLMETTLADLVEKAGKREPMWYI"
     gene            complement(1507..>2227)
                     /locus_tag="VFV87_08430"
     CDS             complement(1507..>2227)
                     /locus_tag="VFV87_08430"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5103821.1"
                     /translation="TPELLAELAAASSRLETASPEQIIAWGVEHYAPYLTMATAFGPE
                     GCVILAMLSKIAPETYVFNLDTGYQFQETIELRDRIAEKYGIEVDLLTPDLSVPEYEA
                     LHGGPLYRTNPNQCCFDRKIKTLQKASQVMHAWMSGIRRDQSPDRAQAAVVGWDKKFG
                     LVKISPLANWTKKDVWKRITDEQVPYNPLHDQGFPRIGCWPCTRAVAAGETDERAGRW
                     SGMKKTECGLHSVNEQDGSGL"
BASE COUNT          454 a          711 c          630 g          432 t
ORIGIN      
        1 gagcatccac agcggataga gttcgcccat ggcatcgcgc gaccagcggt ggacatcgaa
       61 gtcgtcggct ttgatccact ccaggaacgg gccacgaagc tcttcgagat cgcagtacat
      121 gacgcccgcg cccccgacca cgccgaatcg atcggcgtcg agcgtcgcct gcgataggca
      181 ggaatcctgc caggcgagtt cagctgcagc tgaggcgagc tgaatatcgc gtgacatcac
      241 cttgagactc ttccgcggct gaaccaactc tttgggatcg aagtcggtca cctcgccacc
      301 aaacggggcc agccagccgg ccgaggctaa ttgctcgatc gggcgcacgc cgctttgccg
      361 cgattcgatc gcgttccaaa ccgcctcgcg cccgacaccg attggcgaga cgatgcccac
      421 gccggtgata acaacttctc gctcagccac gttgctcccc ggagtaccag aggacgccga
      481 cgacccaata aaaacaacca ccgagacagc atctgcacga agtcaagacg gggaaacaag
      541 gagaggggga gacaaggaga ttcatacgtt cgtcatctcc ctgtttcctt gtctccgtat
      601 ctccttgtct attacctccg tgtgctccgc gcctccgtgg ttcgttcttg aaatataagt
      661 ctaattgtca cgcaaagggc cgacaacgac cttgaaactg gcggaaaatg gaacggaaaa
      721 gatggaaacg acggcagacc gaagtgtcgc acttcgaata atctcagcgg aggtcgcaag
      781 aatccctcaa gtcgccgccc gtgggatgga cattagtccg tccggttcga acgggttgaa
      841 gtccgtttca cgatgtgtgg gacaagcagt cgaagccacg caaagaaacc cccagggggg
      901 atttggccgg ctaaaagcgt tggggttgac gttcctgcct gccaaaggag atgtgccggc
      961 agtcagcaat gtcggcccca ccgcctaccg tcagatgtac cacatcggct cacgcttccc
     1021 ggccttttcc accaggtcgg cgagcgtcgt ttccatcaag cggtcgcgct gaatttcgtc
     1081 aagctcgcgg cagagatgga gcagcgaacc gacgagcggc gtgttctttc ccgcattggt
     1141 cgcgggccgt tcatcgcctt ccatcacctc gatcacgtcg gcgatagtga tctcggaggg
     1201 cgaacgtgcg agccggtagc caccggccgc tccgcgcgtg ctcaccacca gcgaagcccc
     1261 cttgagctgc aacagaatct gcaccaaaaa ccgcgacgga attccatgca catcggcaat
     1321 ccgccgaatc tgcaggggct cacccttcgg gtactcggcc gccagctgca gcatggccag
     1381 gcacgcgtac tcggtcttcg cggagagctt catggggagg gcctgcgggc ggacgttggg
     1441 cggatgaata ctcagccacc actacgccac tctaaaccct caaccctcag ccaacaaccg
     1501 cgaatatcaa agtccgctgc cgtcctgctc gttaacggag tgcagtccgc actcggtttt
     1561 cttcattccg ctccagcggc cggctcgctc atcggtctca cccgccgcca ccgcccgcgt
     1621 gcacggccag cagccaatac gggggaaacc ctgatcgtgc agcggattgt acggcacctg
     1681 ctcgtcggtg attcgtttcc aaacgtcctt cttcgtccag ttcgcaagtg ggctgatttt
     1741 cacgagccca aattttttgt cccagcccac gactgcggcc tgcgcccggt cgggactctg
     1801 gtcgcggcga ataccgctca tccatgcgtg catcacctgc gatgccttct gcagcgtttt
     1861 gatcttccgg tcgaagcagc attgattcgg attcgtgcgg tatagcgggc cgccgtggag
     1921 cgcctcgtac tcgggcactg agaggtccgg cgtcaacaaa tcgacctcta tcccgtactt
     1981 ttccgcaatt cggtcgcgca gttcgatcgt ttcctgaaac tggtagcccg tgtcgagatt
     2041 gaacacatag gtttccggcg cgattttgga aagcatcgcc aaaatcacgc agccctcggg
     2101 gccaaacgcg gtcgccatcg tcaggtacgg agcgtagtgc tcgacgcccc aggcgatgat
     2161 ctgttccggc gaggctgttt ccaggcggct gctggcggca gccagctccg ccagcaattc
     2221 cggagtc
//
LOCUS       DASUGB010000434         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_466849, whole genome shotgun sequence.
ACCESSION   DASUGB010000434 DASUGB010000000
VERSION     DASUGB010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466849"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..2103)
                     /locus_tag="VFV87_08435"
     CDS             complement(22..2103)
                     /locus_tag="VFV87_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEX5103822.1"
                     /translation="MLSKLPALVPCLLTLAGIVVGPYSPSQAAADDSTAVRSHPPMRL
                     LPEAPKRDIAGGERRFVDAARGDDTAAGTETAPWKTLTHALRQLQPGTTLYLRGGRYY
                     EKASLTRSGTEGAPITIASYPGEIAVLDGGLREFFESPDSSWEPLAGGAEGEYVSTKT
                     YFDADSRRAPTQFLPGSWEPMWGIENERPLALGHFADSMVPLHGYRLLVDLRSQNELW
                     IGGKGEMRDMGIYCGPGLWLNRDTGRIHIRLAHHRLAGLGGHAYRGETDPRNLPLVIA
                     AGFGDDVLRISGMQHVRLHDLVLCGATGSPMINIYGSQHVELDGLTVYGGFPGLLLNA
                     SQEIRVVNCAFRGLAAPWTSRAHMKYRGTASYQIVFQNQQPVNENIEIANCEFTDDHD
                     FAFLRNVKNLRFHHNFVDNFNDDGLECGPKLRDHTLYISQNRIGGCLIPLTQHELSKD
                     DSPLDHDPGSGVFIFRNVFDLRGGTYKGPPAEPDPSGAFLHGEGHLCGDHGGPVWAIY
                     RAYHNTFLRETPVFRDYFLMGLGVQGLRNNERDVFNNIFVQTDKVPGVGFAGITQLGI
                     VREGGNLIWGLAEGPTLQVDPFAKFRASKLFDESHKIYGPGWTTHDRAADPKFVSLSV
                     DRSEPFDLRLQAGSPAIDFGQPLPAEWPDPLRELDSGPPDAGALPHGSKVWGVGVGGR
                     IPLFGNAGGVK"
BASE COUNT          414 a          660 c          701 g          405 t
ORIGIN      
        1 tagttctgct tggggcgccg ctcatttgac tccgccagca ttaccgaaga gcggtattct
       61 ccccccgaca cccactcccc agactttgga gccgtgcggg agcgcacccg catcgggggg
      121 gccagaatcg agctcgcgca aggggtccgg ccattcggct ggaagcggct ggccgaagtc
      181 gatcgccgga cttcccgctt gcaggcgcag atcgaacggc tcgcttcggt ctacagacag
      241 gctgacaaac ttggggtcgg ccgctcggtc gtgcgtcgtc cagccggggc cgtagatttt
      301 gtgactttca tcgaacagct tcgacgcgcg gaacttggcg aagggatcaa cttggagcgt
      361 cggtccctcc gccagccccc aaatcagatt gcctccctca cgaacgattc cgagctgtgt
      421 tatccccgca aagccgacgc cgggcacttt gtcggtctgg acgaaaatgt tgttgaagac
      481 gtcgcgctcg ttgtttcgta gaccctgcac accgagtccc atcagaaagt agtcgcgaaa
      541 gacgggcgtt tcgcggagga atgtattgtg ataggcgcga tagatggccc acaccggacc
      601 gccatgatcg ccgcacaggt gcccctcgcc gtgcaaaaac gcgccgctgg ggtcgggctc
      661 ggcgggcggg cccttgtacg tgccgccgcg gaggtcgaac acgttgcgga agatgaacac
      721 gcccgagccc ggatcgtggt cgaggggcga atcgtccttg gatagctcat gctgcgtcag
      781 cggaatcagg caaccgccga tgcggttctg cgagatgtac agcgtgtggt cgcggagctt
      841 gggaccacat tccaggccgt cgtcgttgaa gttatcgacg aagttgtgat ggaaccgcag
      901 gttcttgacg ttccgtaaga atgcgaagtc gtgatcatcg gtgaattcgc agttggcgat
      961 ctcgatgttc tcgttcaccg gctgctggtt ctgaaaaacg atttgatagc tcgccgtgcc
     1021 gcgatacttc atgtgggccc gcgaagtcca tggtgcggcc aggccgcgga atgcgcaatt
     1081 caccacgcga atttcctgcg acgcattgag gagcagccca ggaaagccgc catagacagt
     1141 caggccgtcg agttccacgt gctgcgatcc atagatgttg atcatggggc tgccggtcgc
     1201 tccgcagagc acgaggtcat gcaggcgcac gtgctgcatg ccactgatgc gaaggacgtc
     1261 gtcgccgaaa ccagcagcga tcaccagcgg aaggttgcgg gggtcggtct cgccgcgata
     1321 ggcgtgccct cccaggccgg cgagccgatg atgcgccagg cggatgtgaa tccggccggt
     1381 gtcgcgattg agccataggc ccgggccgca atagatgccc atatcgcgca tttcgccctt
     1441 gccgccgatc cataactcat tctgcgagcg caagtccacc agcagccggt agccgtgaag
     1501 cgggaccatg gaatccgcaa aatgaccgag cgccagcggc cgttcatttt cgattcccca
     1561 catcggctcc cacgagccag gaaggaactg agtcggcgcg cggcggctgt cggcatcgaa
     1621 gtacgttttc gtcgagacgt attccccctc ggcgcctccg gcgagaggct cccagcttga
     1681 atcggggctc tcaaaaaact cccgcaagcc gccgtcgagg acggcgatct cgcccgggta
     1741 cgaagcaatc gtgatcggtg ccccctccgt gcccgacctg gtcaggctgg ccttctcgta
     1801 atagcggccg ccgcgcagat agagggtcgt ccccggctga agctggcgga gcgcatgagt
     1861 cagcgtcttc cacggtgcgg tttctgtccc cgctgcggtg tcgtcgccgc gcgccgcatc
     1921 gacaaaccgc cgttcgccgc cggcgatatc tcgttttggc gcttccggaa gcaggcgcat
     1981 cggcggatgc gagcgcacgg cagtggaatc gtcggctgca gcctgggacg gagaataagg
     2041 gccgacgacg atcccggcaa gcgtcagcag acagggaact agggcgggca atttcgatag
     2101 catgttgaac tccagaattc aggtgggatg ggtacaacct ggagtggcgg gggagtgcag
     2161 cacccgtatt ctaactgcaa
//
LOCUS       DASUGB010000435         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_883946, whole genome shotgun sequence.
ACCESSION   DASUGB010000435 DASUGB010000000
VERSION     DASUGB010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883946"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..438)
                     /gene="cutA"
                     /locus_tag="VFV87_08440"
     CDS             complement(103..438)
                     /gene="cutA"
                     /locus_tag="VFV87_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5103823.1"
                     /translation="MSAEAIQVVTTTTERKDADALAKAVLDKRLGACVQVSGPIDSSY
                     WWNNRLEQAREWTLTIKTRRDLYKPLEKLLLELHPYDQPEIIATAVVEISSGYRLWLD
                     EQLKPPKKG"
     gene            complement(580..1716)
                     /gene="ispG"
                     /locus_tag="VFV87_08445"
     CDS             complement(580..1716)
                     /gene="ispG"
                     /locus_tag="VFV87_08445"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEX5103824.1"
                     /translation="MEIPRNPTRSVRIGSVTIGAGQPIAVQSMTATHTQDIDATVGLV
                     NLLDAAGADVVRIAVDSKKDAEALAEIRRQTKANLSVDLQENYRLAELVAPHVDKVRY
                     NPGHLYHHEREKPWQEKVRYIAAVARDNDCALRVGVNCGSVDPAKKEKYAEDDSITPM
                     LESAWEHCELLDSLDFTRYCVSLKDSDPAKVIEVNRRFAERRPDVPLHLGVTEAGLPP
                     DGVIKTRIAFEQLLSRGIGDTIRVSLTVPNDRKPEEIQAGRQILADIAAGRVRSVVNY
                     GLPTLNIIACPSCSRVENQAFIDLAQQVKEMTRYAEKHAITIAVMGCRVNGPGETDDA
                     DLGLWCGPTHVNLKRGSAELGAYPYDQILPRLKWELDALIASRG"
     gene            complement(1796..>3101)
                     /locus_tag="VFV87_08450"
     CDS             complement(1796..>3101)
                     /locus_tag="VFV87_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103825.1"
                     /translation="AEFEVDDVAEWSKAVATLASAAKDEPSMGAVLEETLPIVRQALA
                     AQQFEAAQRLLESLTRMAQRPAGRKLLPQIAALREQSADAERLARGLQAARQKLEADP
                     DDPDANLAVGASLCFSHGDWQGGLPHLAIGADAALQAAAGRELAGAKTPEDELAVGDA
                     WWDAGDKAAKEYRSAAISRAAGWYRRARPGVASGLAQAKIDKRLAEAGPLAASSEPAG
                     AKGPAGSTAGKGVLLVLHIGEEQGRVPAMCQKANLPIEVASNFDLNRTDYAKFHTLLA
                     GSNAMDYFGQDHTRQPEAFGHIERFVAEGGHLVLFGVFDGRNCENLARFGVYTEARHA
                     ETFQSVGRATELLLQDVEPLVPKDGRMKSCGAIQCAVPHEVLLRIGLGRREGETAMIS
                     LPYKAGRVTFTECEPHAAGDWWLMEAVIGWIARGAPLPAGSP"
BASE COUNT          485 a         1062 c          977 g          577 t
ORIGIN      
        1 gtcaattgcg acaaccgctc tcgatgtgga gaacaaagac cgcaattgac tcccgacccc
       61 gtcccgtttg ccactcgtac cgcgcgaacc actgcccatg actcacccct tctttggcgg
      121 cttgagctgc tcgtcgagcc acaatcggta gccggagctg atttcaacca ctgccgtggc
      181 aataatttcc ggctggtcgt agggatgcag ctccaggagc agcttttcca gcggcttata
      241 tagatcgcgg cgggtcttga tcgtcagggt ccactcgcgg gcctgctcca ggcggttgtt
      301 ccaccagtaa ctgctgtcga tcgggccgct cacctgcacg cacgccccca gccgcttgtc
      361 gagcacggcc ttggccagcg catcggcatc cttccgttcg gtggtcgtcg tcacaacttg
      421 gattgcttcg gcactcatgc gggcattgtg ccacgcccgg ccgcgttggg cgaggggctg
      481 atcgtaggct tctcgctccg cgagaagcag aaggcgctct tggcgctccc gcgcggatcg
      541 caacttgcgg agcgagtcgc ctacgatgcg gcagacggac tacccgcgag acgcgatcaa
      601 ggcgtccaat tcccacttca gccgcgggag gatctggtcg tagggatacg caccgagctc
      661 ggcgctgccg cgtttgagat tgacgtgcgt cggcccgcac cagaggccga ggtcggcgtc
      721 gtcggtttcg ccggggccgt tcacgcggca gcccatgacg gcgatcgtaa tcgcgtgttt
      781 ttcggcgtag cgggtcattt ccttcacttg ctgggccaag tcgatgaacg cctggttctc
      841 gacccgcgag cagcttgggc aggcgataat gttcagcgtc ggcaggccat agttgacgac
      901 gctccgcacg cggcctgcgg cgatatcggc cagaatctgc cgcccggctt gaatctcttc
      961 gggcttgcgg tcgttgggca cggtcagcga gacgcggatc gtatcgccga tcccgcggct
     1021 gaggagctgc tcgaaggcga tgcgggtctt gatgactccg tccggcggca gaccggcctc
     1081 ggtcacaccc aggtgcagcg gcacatcggg ccgtcgctcg gcgaagcggc gattgacctc
     1141 gatcaccttg gccgggtcgc tgtccttgag cgaaacgcaa taccgcgtga agtcgagcga
     1201 atccaggagc tcgcaatgct cccaggcgct ttcgagcatc ggggtgatcg aatcgtcctc
     1261 ggcgtacttc tccttcttcg ccggatcaac gctgccgcag ttcacgccga cgcgcagggc
     1321 acagtcgtta tcccgcgcaa cagcagcgat gtagcgcacc ttttcctgcc acggcttctc
     1381 gcgctcgtgg tgatagaggt gccccgggtt atagcggact ttatcgacgt gcggcgcgac
     1441 caattccgcc aggcgataat tctcctgcag atcgaccgac aggttggcct tcgtctgccg
     1501 gcgaatctcc gccagcgcct ccgcgtcctt cttgctatcg acggcgatcc gcaccacatc
     1561 cgcccctgcc gcatccagca ggttcaccag ccccacggtc gcatcaatgt cctgcgtatg
     1621 cgtcgccgtc atgctctgca cggctatcgg ctgcccggcg ccgatggtca cggagccgat
     1681 gcggacggag cgggttggat tgcgggggat ttccatgcgt ggggcaggcc tccagcctgc
     1741 catcagagtt cgacgcctgc tgccattctg tccaaccgca gcccaccggg caatgtcacg
     1801 gcgaaccagc cggcagcggc gctccgcggg cgatccagcc gatgaccgcc tccatgagcc
     1861 accagtcgcc ggcggcgtgc ggttcgcatt cggtgaaagt gacgcggccc gccttgtagg
     1921 ggagggaaat catggcggtc tccccctcgc ggcggccgag gccgatccgt agaagcactt
     1981 cgtgcgggac cgcgcactga atcgccccgc agctcttcat gcggccgtcc ttaggaacca
     2041 gaggctccac gtcctgcagc aacagttcgg tcgcccggcc gaccgattga aacgtctccg
     2101 cgtgccgggc ttcggtatag acgccaaagc gggcaaggtt ctcgcagttg cggccgtcga
     2161 agacgccgaa cagcacgaga tggcccccct cggcgacgaa ccgctcgatg tgcccaaacg
     2221 cctccggctg gcgggtgtga tcctgaccga aatagtccat cgcgttgctg ccggccagga
     2281 gcgtgtggaa cttcgcatag tccgtgcgat tcaggtcgaa attgctggcg acttcaatcg
     2341 gcaggttcgc cttctggcac atcgcgggca cgcggccctg ctcctcgcct atgtggagca
     2401 ccagcagcac gcccttgccg gccgtgctgc ctgcgggccc ttttgcgccg gcaggttcgc
     2461 tcgatgcggc cagcggtccg gcctcggcca gacgcttgtc gatcttggcc tgcgcgagcc
     2521 cggatgcgac ccccggccgc gcccggcgat accagccagc cgcccggctg atggcggcgc
     2581 tgcgatattc cttggccgcc ttgtcgccgg catcccacca ggcatcgcca actgccaatt
     2641 cgtcttccgg agttttggcc ccggccaatt cgcgcccggc agcagcctgg agcgccgcat
     2701 cggcccctat ggccagatgc ggcaggccgc cctgccaatc gccgtggctg aagcagagcg
     2761 acgcgccaac cgccaggttc gcatccgggt cgtccggatc ggcctccagt ttttgccggg
     2821 ccgcctgcag gccgcgggcg agacgctcgg cgtcggctga ttgctcgcgc agcgcggcaa
     2881 tttgcggcaa cagcttgcga ccggcaggcc gctgggccat gcgcgtcagg ctctcgagaa
     2941 gccgctgggc cgcctcaaac tgctgcgcag ccagagcttg ccgcacgata ggcagcgttt
     3001 cttccagcac ggctcccatc gaaggctcgt ccttggcggc gctggcgagc gtggcgaccg
     3061 ccttgctcca ttcggccaca tcatcgacct cgaattccgc g
//
LOCUS       DASUGB010000436         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1855127, whole genome shotgun sequence.
ACCESSION   DASUGB010000436 DASUGB010000000
VERSION     DASUGB010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855127"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VFV87_08455"
     CDS             <1..1078
                     /locus_tag="VFV87_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5103826.1"
                     /translation="ITATGDSHINGPANFHRCAGDARAEAEHVAETLLAVRLRCANCH
                     NHPLDRWTQDDYHGLAAIFAPLDRGQFVGFRARGEVIHPATGEAAAPRIPGERFLDRA
                     TDGRQALAAWLASKNNPYFARAWVNRFWQALMGRGLVEPIDDFRDTNPGTHPRFLTRL
                     AEDFSRDFDFYHLVRTIVSSAAYQRGRSPGVPDDRYYSQALSRPIPTPVLIRLICQAT
                     EVPGERLDEARAKDQEGELQSLLERHPFGVLACAAGPASGKGEYQVASGPADLSGELQ
                     WLNGPLLNRKLADRRSAFFKIAASGVTNQRVIEEYYLRTLSRLPTAVELEHWDAELNT
                     ADRNNRCEDFAWSLLSCPEFVTNH"
     gene            1133..>2159
                     /locus_tag="VFV87_08460"
     CDS             1133..>2159
                     /locus_tag="VFV87_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103827.1"
                     /translation="MDLQSAIRNPQSAIPNCPSRRDFIRIGGLTALGLGLIDFLALRQ
                     ASAAAAPPMATRTVLIWLDGGPSHLETFDLKPDAPVEARGPFQPTSTNVPGIQICDLF
                     ERLAQRMDRAAMVRSVTSPLGEHNLGTHYMLTGYPPTPALEYPAIGSVVAHLDATNER
                     TLPSHIAVPNFGVGGKRISGNGYLPTQARPYEVGGDPSKPDFRVENLDFFAGVTSERI
                     DRRRDYLAALDRLSRDADAGEAPNPRPFEQAFRLITSKEAKSAFDLTKETPATRARYG
                     PRTIGQSTLLARRLVEAGVPFVTVNYTGWDTHESLYTRLKEGYTGAKQPVGLIPSLDL
                     ALSALLDD"
BASE COUNT          371 a          721 c          665 g          402 t
ORIGIN      
        1 gatcaccgcg accggtgatt cgcacatcaa tggccccgcc aatttccacc gctgtgccgg
       61 cgacgcccgc gccgaagccg agcacgtggc cgagacgctg ctggccgtgc ggctgcggtg
      121 tgcgaattgc cacaaccatc cgctcgaccg ctggacacag gacgattacc acggtctggc
      181 agcgattttc gccccgctcg accgcggcca gttcgtcgga ttccgcgccc gcggcgaagt
      241 cattcacccc gccaccggcg aagccgctgc gccgcggatt cccggcgaga ggtttctcga
      301 ccgagcgacc gacggtcgcc aagcgctcgc agcgtggctg gcgtccaaga ataaccccta
      361 cttcgcccgg gcctgggtaa atcggttctg gcaggcgctc atgggccgcg ggcttgtcga
      421 gccgattgac gacttccgcg acaccaatcc gggtacccat ccacgttttc tgacgcggct
      481 cgcggaagat ttttcccgcg actttgattt ttatcacctc gttcgaacga tcgtttcctc
      541 cgcggcttat cagcgcggcc gatctccagg agtgccggac gatcgctatt attcgcaggc
      601 gctgtcccgg cccattccaa cgccggtttt gatccggctc atctgccagg cgaccgaagt
      661 tccaggcgag cggctcgacg aggcccgcgc caaggaccag gaaggcgagc tccaatcgct
      721 gctggagcgg cacccattcg gcgtgctggc gtgtgcggca ggccccgcta gcggcaaggg
      781 cgagtatcag gttgcgtccg ggcccgcgga tctgtcgggc gagctgcagt ggttgaacgg
      841 cccgctgctc aaccggaagt tggccgatcg gcgaagcgcg tttttcaaaa ttgctgcttc
      901 tggcgtgaca aatcagcgcg ttattgaaga gtattatctg cggaccctgt cacgccttcc
      961 gacggcggtg gaacttgagc attgggatgc tgagctgaac acggctgatc gcaataaccg
     1021 atgcgaagac ttcgcctgga gcctgctgag ttgcccggag tttgtgacga atcattaggg
     1081 gcgggggcaa gtgagtggtg aataggtgag tggtgaaatg tggaggttca caatggatct
     1141 gcaatccgca atccgcaatc cgcaatccgc aattccgaat tgcccttcgc gccgcgattt
     1201 catccgcatt ggcgggctga ccgcgctcgg gctggggctg atcgattttc tcgccttgcg
     1261 gcaggcgtcg gcagccgccg cgccgcctat ggcaacccgt acggtcctga tttggctcga
     1321 cggcgggcct tcgcacctgg aaacgttcga tctcaagccc gatgcgccgg tcgaagctcg
     1381 cgggcccttc cagccgacga gcaccaacgt ccccggcatt cagatctgcg atctcttcga
     1441 gcgcctggcg cagcgaatgg accgggcggc gatggtccgc tcggtcacgt ctccgctggg
     1501 tgagcacaac ctcggcacgc attacatgct caccggctat ccgccgactc ccgccctcga
     1561 atacccggcg attggcagcg tggtcgccca tctcgatgca actaacgagc gtacgctgcc
     1621 cagccacatc gccgtgccca acttcggtgt tggcggcaag cggatcagcg gcaacggcta
     1681 tttgccgacg caggctcgcc cgtatgaagt cggcggcgat ccctccaagc cggacttccg
     1741 cgttgagaat cttgatttct tcgccggcgt gaccagcgaa cggatcgacc gccgccgtga
     1801 ctatctcgcc gcgctcgacc gcctcagccg cgatgccgac gcgggcgagg ctcccaatcc
     1861 aaggccgttc gagcaggcct ttcgcctgat cacctcgaag gaggccaagt cggccttcga
     1921 tctcacgaag gaaacgccgg ccactcgcgc ccgctacggc ccccgaacga tcggccagag
     1981 cacgctcctc gcccgccgcc tcgtcgaagc cggcgtgccg ttcgtgacgg tcaattacac
     2041 cggctgggac acgcacgaaa gtctctacac ccggctgaag gagggctaca ccggtgcgaa
     2101 gcagccggtg gggcttattc cgtcgctgga tctcgcgctt tcggcgctgc tcgacgatc
//
LOCUS       DASUGB010000437         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1995351, whole genome shotgun sequence.
ACCESSION   DASUGB010000437 DASUGB010000000
VERSION     DASUGB010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995351"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFV87_08465"
     CDS             complement(<1..225)
                     /locus_tag="VFV87_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103828.1"
                     /translation="MSCPPEVLALLQRFADNYDEYQDPAYKEAHLRQEFIDPLFAALG
                     WDMDNRSGKAEAYKDVIHEDAIKVGGGHKAP"
     gene            849..2819
                     /locus_tag="VFV87_08470"
     CDS             849..2819
                     /locus_tag="VFV87_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5103829.1"
                     /translation="MKTLFRVAGVALVLAAGVSTAQDVQELEEQAVLAAAGAVAPSVV
                     KIETLGGLERVGQVLVGTGPTTGLAVSGDGYVISSAFNFIQQPSSILVTLPSGNRAAA
                     TIVARDHSRMLVLLKVNAKESLVVPQAVPRDEIRTGQWSIAVGRTFDQPEPNLSVGVI
                     SAVNRIWGKAIQTDAKISPANYGGPLIDIHGHVLGVLVPLSPQGQASEVAGAEWYDSG
                     IGFAVPLAEINERLEALKSGKDLHAGLLGVSLKPGDPYALPAEVAACQAGSPAYKAGL
                     RAGDTIVELDGTPIRRQVQLKHALGPRYAGDKVHVVATRGKENVERFEADIELAEKLI
                     PYDHPFLGLLPMRDAAEGGVAVRYVYSGSPAAEAGLKAGDKIVALDQTAVVDAAQLRN
                     LVANLEPKTKTTLKIERGGESLSLELVPASLPTEIPAELPPARAKEPAAVDPAAAPPS
                     VGIVEIKLPEEAAQCVAYVPENYHPSVPHGLVVVLSPAGPVDQVKLAESWKDVCEERQ
                     LIVLAPMSAAADKWQPTETAFVRKTIDDVVGRYNIDPLRIAAYGYQSGGSMAYLVGFE
                     HVDRIRAIAVVDAIPPARAKAPDSDPINTLAFYLAVAEKSRVAAGIKALVARLEAAKL
                     PVTQKSLGEQPRELSAEELAELSRWIDALDRI"
BASE COUNT          523 a          776 c         1049 g          505 t
ORIGIN      
        1 gggggccttg tggccgccgc cgactttgat cgcgtcctcg tggatgacgt ctttgtaggc
       61 ctccgccttg cccgagcggt tgtccatgtc ccagccgagg gcggcgaaga gggggtcgat
      121 gaactcctgg cggaggtggg cctctttgta ggcggggtcc tggtactcgt cgtagttgtc
      181 ggcgaaccgc tggaggaggg ccagaacttc gggagggcag gacatgggga aggattgtaa
      241 aagggcgcgg acgggggagg cgagccaagg ggaaagaatt tgaggaattt tgaggtgcgg
      301 gggaaataat cgggcggggt cgcgcatcta tacatttgtg gcgaattgcg cgcgggcagg
      361 ttctggcaag ctggcggaat tggcaagttg cggctgagcg ggccgcatgg agatcgaggc
      421 gaaggattcc tcgcttgcgc gtcgggttgg tgtgtgggca gtttcaagcg accgaagaaa
      481 cggcgaaaaa atggttgaca aagcgcaact gcgcaacatc ctcatgtggg gggtgttgcg
      541 cagcgcaagt catttgtcat acaggagtta gcaaaatgtt gcgcggggag cagcggcgcg
      601 aaaaaaatgg ggaagttgcg cgctgcgctt tgcccgcgat cgcagcgagc gaccaaaaaa
      661 actggccgaa agcgcatcac gggggtagag aatcgtaggg aaggtgcaaa ggccatagag
      721 ggagtgtcat ttgcctgcgg cggcgggtcg gctcgaactg ccccgccggg ctccctggcc
      781 tggcaagttg aaaagtcggg tcggacaatt cgcgatcggc tagaatggcg aagatgaaga
      841 ctttattgat gaagacccta tttcgtgttg cgggcgttgc gctggtcctt gcggctggag
      901 tttcgactgc ccaggacgtg caggagctgg aggagcaggc agtgcttgcg gccgcgggcg
      961 cggtcgcgcc ttcggtcgta aaaatcgaga cgctcggcgg gctggagcgg gtggggcagg
     1021 tgctggtcgg aacggggccg acgaccggcc tggccgtctc gggagacggc tacgtcatct
     1081 ccagcgcgtt caactttatc cagcagccgt cgtcgattct ggtcacgttg cccagcggca
     1141 accgggctgc agcgacgatc gtcgctcgcg accacagccg gatgctcgtt ttgctgaagg
     1201 tgaacgcgaa ggaatcgctg gttgtgccgc aagcagtgcc gcgcgacgaa atccgcacgg
     1261 ggcagtggtc gatcgccgtt gggcgcacgt tcgaccagcc ggagccgaat ctttcggtgg
     1321 gcgtaatcag cgcggtgaac cggatctggg gcaaagcgat tcagaccgac gcgaaaatct
     1381 cgcccgcaaa ctatggcggg ccgctgatcg acattcacgg ccatgtgctg ggcgtgctcg
     1441 tccccctttc gccgcaaggc caggcgagcg aagtggccgg cgccgaatgg tacgactcgg
     1501 gcatcggttt cgccgtgccg ctggcggaga tcaacgagcg cctggaggcg ctcaagagcg
     1561 gcaaggactt gcacgccggg ctgctgggcg tttcgctcaa gccgggcgat ccctatgctc
     1621 tgccggcgga ggttgccgcc tgccaggcgg gttcgccggc atacaaagcg gggctgcgag
     1681 cgggcgatac gatcgtcgag ctggacggca cgccgattcg gcggcaggtg caattgaagc
     1741 acgccctggg gccgcgttat gcgggcgaca aggtgcatgt cgtcgcgacg cgaggcaaag
     1801 agaacgtcga acggttcgag gccgatatcg aattggccga gaagttgatt ccctacgacc
     1861 atccttttct gggcctgttg ccgatgcgcg atgcggcgga agggggcgtg gcggtgcggt
     1921 atgtctattc cggcagtccc gcggcagaag ctgggctgaa ggcgggggac aagatcgtgg
     1981 cgctggatca gacggccgtc gtcgatgcgg cccagcttcg gaatctcgtt gcgaatctgg
     2041 agccgaagac gaaaacgacg ctgaaaatcg aacgcggcgg cgagtcgctc tcgctggagc
     2101 tggtgcccgc gtcgctgccg acggagatcc cggctgaatt gccgccggcc cgcgccaagg
     2161 agccggctgc cgttgatccg gccgccgcgc cgccgtccgt gggcatcgtg gagatcaagc
     2221 tgcctgaaga agcggcccag tgcgtcgcct atgtgcccga gaactaccat cccagtgtgc
     2281 cgcacggcct ggtggtggtg ctctcgccgg cggggccggt cgatcaggtg aagctggccg
     2341 aaagctggaa ggatgtgtgc gaggagcgcc agctcatcgt gctcgcgccg atgtcggccg
     2401 cggccgataa gtggcagccg accgagacgg cgttcgttcg caagacgatc gacgacgtag
     2461 tggggcgcta taacatcgac ccgttgcgga tcgcggcata cggatatcaa tcgggcggtt
     2521 cgatggccta cctggtgggc tttgagcacg tcgatcgcat ccgggccatc gcggtcgtcg
     2581 atgccattcc gccggcgcgg gccaaggcgc ccgatagcga cccgatcaac acgctggcgt
     2641 tttatctggc ggtggcggag aagtcgcgtg tcgctgctgg cattaaagcg cttgtcgccc
     2701 ggctggaggc agccaagctg ccggtgacgc agaagtcgct gggcgagcag ccgcgcgagc
     2761 tatcggccga agagctagcg gagctgtcgc ggtggattga cgcgctggac cgcatataag
     2821 cggacagaca ttcttgcctg ccaaggactt atg
//
LOCUS       DASUGB010000438         9692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1161573, whole genome shotgun sequence.
ACCESSION   DASUGB010000438 DASUGB010000000
VERSION     DASUGB010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161573"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFV87_08475"
     CDS             complement(<1..380)
                     /locus_tag="VFV87_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103830.1"
                     /translation="MHTLVLIGVLCCGEISEKDLLARLDASYAKLLEDEPSAAEVQRA
                     ARLLAETNQRDRWTNYSAATKILRQTRPQAGIPLLLAYMVRHAELSTGHVVIPEYAET
                     LAVLTGKEVANPYRYEADRKAPVLG"
     gene            complement(525..1940)
                     /locus_tag="VFV87_08480"
     CDS             complement(525..1940)
                     /locus_tag="VFV87_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103831.1"
                     /translation="MLARRTILLLIAALALVGPHGDVRAENIDLSTVPSRETVQLTIY
                     NSEDLTLVRETRKVTFKKGANPLQFSWANTLIDPTSVELKFLAQADKLLVLDTTFPHD
                     KPQMLYWNVQSDFDGEATIEISYFTSGITWTADYLAMANAEETATRLEGFVRVHNNSG
                     EEYEDAQVRLVVGTINLVQKIAQLAQIPMASVQNMPANDFAPAKGAAARMLFDRADKQ
                     AQESGEKAIVKEGLSEYFIFTIEGTETVPNGWSKRLRSIEAAAVPVKIQYRYRPVEYG
                     DQLVRMYLLTNDEKSQLGTAPLPDGVVRVFRDNGRGGLSYITQQSIKYIPIGDKIELN
                     LGVDPEVIFELVKLRAFRTDLWMQVRGARELRKVGGDAVLREEIASLAGWDDHEVFSQ
                     RIRNYTGKEIEVEVRRSFPGHVIFKSQLEPVLFDYQTPQFLARIAAGRRADLLFEIVR
                     KQGTSAKQNNVTLETAAVAAR"
     gene            2138..3592
                     /locus_tag="VFV87_08485"
     CDS             2138..3592
                     /locus_tag="VFV87_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX5103832.1"
                     /translation="MADYPKVAQLKNVEAFRARLAALGLDLPVDEDVLTAEQGSPLAA
                     PIQIGPLRVGNRWCIHPMEGWDANRDGSPSELTLRRWWHFGLSGAKLIWGGEAAAVQP
                     DGRANPNQTLAVPSNKAGLAALWNECLAAHRRAFGPNATDDLVVGLQLTHSGRFCRPN
                     SKQLEPRIAYHHPLLDAKFGIRPDDNAVVWSDDDLERLIDSYVVAAGLAREVGYQFVD
                     IKACHGYLLHEFLSARTRPGRFGGDLDGRTRLLFTIISRVRAAFPDLGVFVRVSIFDT
                     LPYKTSREVGQPMEYQQLLPYQFGFGAQADDPRQIDLVEGIELLQRLKAAGVLAANIS
                     CGSPYYNPHIMRPAIFPPSDGYQPPEDPLVGVVRQIHAARACKAAVPELAMVGTGYSY
                     LQDYLPHVAQAVIRAGWIDLVGLGRMVLSYPEMPADTLQRGTTERKKVCRTFSDCTTA
                     PRNGLASGCYPLDPYYKERPEAEELKRVKQEIKG"
     gene            3792..4100
                     /locus_tag="VFV87_08490"
     CDS             3792..4100
                     /locus_tag="VFV87_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX5103833.1"
                     /translation="MHVIARATLDAFGRKHRAAKEWLENWWQTAVRANWRNLDDVRRV
                     YQSTDQVGGCLVFDCKGNNYRLIVRVSYANKYTRGTLFVKHLLTHAEYNKNRWKDCCE
                     "
     gene            4097..4531
                     /locus_tag="VFV87_08495"
     CDS             4097..4531
                     /locus_tag="VFV87_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5103834.1"
                     /translation="MTTATLDRIVASRSYETLVSRFPPRPIREPGLLKQTYAVIDRLM
                     AIPHPSPFHLEFLELLSTLVEDYESVHYPTPVTSTESLLAHLIESRGVTQTQVAKETG
                     ISPSIISDVLAGRRSLSLPAIVKLSQYFRLDPGAFLRVARDM"
     gene            4701..5723
                     /locus_tag="VFV87_08500"
     CDS             4701..5723
                     /locus_tag="VFV87_08500"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5103835.1"
                     /translation="MSKFCIHPLLVVSCLLVIQASGSSAADQDSGEQLLPANPAMWLN
                     SPPLTSDTLKGKGVVLWFYEEQCPRCRERWPGMYDVAKKFDGKPVVFIAVNSGNPRDE
                     VEQYAKGVKLPWPVIVDQSREFEKQWLDSEISLQNIHQLALILPDGRKQMANWNDFEG
                     AVQKAAEGATWKIDPQTIPAAFQPTWRLVEMGKYDQAAAMVKKGLTTSNAEVKEAATR
                     VHEFVQQELKKSAERAAKLRQEGDTWNAYQAYREIAASFAGYELPLEVSAAQKELAAD
                     EKVKQQLDAARALESIKKSFPTARTDIARKRIVRRLEQLVELFPDSEAAVEAKSILEQ
                     AAEQQQ"
     gene            5899..8718
                     /locus_tag="VFV87_08505"
     CDS             5899..8718
                     /locus_tag="VFV87_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5103836.1"
                     /translation="MPRYTPATIEPKWQRYWDEHKTFKTPEMPPAGSEKLYVLDMFPY
                     PSGSGLHVGHPEGYTATDIVCRFARMNGKCVLHPMGFDAFGLPAEEHAIRTGQHPRVN
                     TEQNIATFTRQLKMLGFSYDWDRNLATTDVEYFRWTQWIFLVLFDTWFDADQQKSRPI
                     SELPIPPAVISKGDIAIRKYQDEHRLAYQSDALVNWCPELGTVLANEEVIDGKSERGS
                     HPVVRMPLKQWMLRITAYADRLEKDLEPLDWSPGIKKLQRDWIGRSVGAEVDFYVGRA
                     SRLPEKTEHRGQRTEDSEFDAWKASRAKSGLPKKTGDDVLRIYTTRPDTLFGATYMVI
                     APEHPFVARLTTPDQKAAVEAYVQTAARKSDRERQEETKKKTGVFTGSYAINPVNGQP
                     VPVWVADYVLWGYGTGAIMAVPAHDERDFDFAMQFGLKIVPVIDPGDISDDDIYPELT
                     QVLSTQYSVLSTDAESADNPKSKIQNPKSPPARQRVLAGQACFAGEGVSINSGPYNGR
                     TTADFKQRITADLDAGGLGREAVNYKLRDWLFSRQRFWGEPFPILHELDASGNPTGLI
                     RAVPEKDLPVDLPHLDDFKPAAAGASPAPPLAKAPKEWLEPTIAGKRYRRETNTMPQW
                     AGSCWYFLRFIDPKNRQRFLDPAKEKAWMPIDLYIGGAEHAVLHLLYSRFWHKVLFDR
                     GHVSTAEPFQKLVNQGMILGENGEKMSKARGNVINPDDVVRDYGADSLRLFEMFMGPL
                     EAVKPWSTQGVVGVRGFLDRVWRLMVDDKADELALNAAVQKVEPTNEQLRTLHKTIKQ
                     VTSDVQRLEFNTAIARMMEFTNFFTKESVRPHSVMEQFVLILSPFAPHIAEELWQLLG
                     HTQTLAYEPWPKFDDALTKDAEVEIPVQILGKLRSKVIVPAGSDQATIIAAARADSRI
                     IELLTGKEVVKTIVVPGKLVNFVVK"
     gene            8760..9035
                     /locus_tag="VFV87_08510"
     CDS             8760..9035
                     /locus_tag="VFV87_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX5103837.1"
                     /translation="MTQATQQVLQAAMALSDDERAELIVHLEDTMAGFATPEVAAAWD
                     AEIAKRLMEIDDGTVELSPAEEVHRRLGEKYGFFADWISSRGHGRAV"
     gene            9312..>9692
                     /locus_tag="VFV87_08515"
     CDS             9312..>9692
                     /locus_tag="VFV87_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5103838.1"
                     /translation="MPDDLITTPAQLEALYGQPGQLVRQMQLSKLEPQSMAFIAQAPF
                     AVLATAGGGGFADVSPKGDAPGFIQVVDETTLHLPDRLGNNRVESLKHILENPRVGLL
                     FFVPGREETLRVHGTARIRIDPELL"
BASE COUNT         1882 a         3056 c         3020 g         1734 t
ORIGIN      
        1 cccagtacgg gcgctttgcg gtcggcttcg tagcgatagg ggttggccac ttccttgccg
       61 gtcaaaacgg ccagcgtctc ggcgtattcc gggatcacca cgtggccggt gctcagctcg
      121 gcgtgccgca ccatgtaagc caacagcagc ggaatgccgg cctgggggcg cgtctggcgc
      181 aagatcttcg tcgcggccga atagttcgtc caccggtcgc gctggtttgt ttcggccaac
      241 aggcgggcgg cgcgctgcac ttcggcggcg ctgggctcat cttccagcag cttcgcatag
      301 gatgcatcga gcctggcgag caaatccttt tcggaaatct cgccgcagca taagacaccg
      361 atcagcacga gcgtgtgcat ggcgttgctc ccctcggggt gacccaaccg ctccacgaac
      421 tcgatttcga tcgtagacga tcggcgccgc gtgcgggagg aagtcgttac gaactgttta
      481 cgccgagtcg ggcgccgggc agcgccctcg ccggccgatt ccggctaccg ggctgcaacc
      541 gcagccgttt cgagcgtcac gttgttctgt ttggcgctgg tgccctgctt gcggacgatc
      601 tcgaacaaga gatcggcgcg ccggccggcg gcaatgcgag ccaggaactg cggcgtttga
      661 tagtcgaaga gcaccggctc gagctggctc ttgaagatca cgtgtccggg aaagctgcgg
      721 cggacttcga cctcgatttc cttgcccgta tagttgcgga tccgctggga aaagacttcg
      781 tggtcgtccc accccgcgag cgaggcgatt tcctcccgca gcaccgcatc gccgccgact
      841 ttccgcaatt cgcgggcgcc gcggacctgc atccacaggt cggtgcggaa cgcgcggagc
      901 ttgaccagct cgaaaatcac ctccggatcg acgccgagat tcagctcgat cttgtctccg
      961 atcggaatgt acttgatcga ctgctgcgtg atgtaggaga gccccccgcg gccgttgtcg
     1021 cgaaagaccc gcaccacgcc gtccggcaga ggcgctgtgc cgagctgcga cttctcgtcg
     1081 ttggtcagca ggtacatccg cacgagctga tcgccatatt ccaccggccg gtagcggtat
     1141 tgaatcttca cgggaaccgc cgccgcctcg atcgaccgca gccgcttcga ccagccattg
     1201 gggaccgtct cggtgccttc gatcgtgaag atgaaatact cgctcaggcc ttccttgacg
     1261 attgcctttt ccccgctctc ctgggcctgt ttgtcggccc ggtcgaagag catgcgcgca
     1321 gcggcgccct tggccggtgc gaaatcgttg gccggcatgt tctgcacgct ggccatcgga
     1381 atttgcgcaa gctgcgcgat tttctgcacg agattgatcg tgccgacaac gagccgcact
     1441 tgggcgtctt catactcttc gcccgagttg ttgtggacgc gaacgaagcc ttccagccgc
     1501 gtggccgttt cctccgcatt cgccatggcc aggtagtccg ccgtccaagt gatgccgctc
     1561 gtgaaatacg agatctcgat cgtcgcctcg ccgtcaaaat cgctctggac gttccagtac
     1621 agcatctgcg gcttgtcgtg cgggaaggtc gtgtcgagga ccagcagttt gtcggcctgg
     1681 gcgaggaact tcagctcgac gctcgtcggg tcgatgagcg tattggccca actgaactgc
     1741 agcgggttgg cgcccttctt aaaggtcacc ttccgcgtct cgcggaccag cgtcaggtcc
     1801 tcgctgttgt agatcgtgag ttgaaccgtc tcgcggctgg gaaccgtcga gaggtcgata
     1861 ttctcggcgc gcacgtcgcc gtgcggcccg accaaggcaa gagcagcgat tagaagcaga
     1921 atcgttcgtc gagcgagcat ggaatgcacc gcgggcaatg ggaatcggca ctgcccaata
     1981 agacgaccag ggtggcactt tggtttccgc cgtggcgaca agcggaagtc ccgtgcctgc
     2041 aacggtttat gaacccccat ttctcggccg acgccggtca ggcgagtctc tgttatcttc
     2101 aggggaacgt ctcacgcttc cgtgttccgt gataccgatg gcggattacc caaaagtcgc
     2161 tcagctcaag aatgtcgagg cgtttcgcgc gcggctggca gcgttgggat tggacctgcc
     2221 cgtcgatgag gacgttttga ccgccgagca gggctcgccg ctggcagcgc cgattcagat
     2281 cggcccgctg cgagtcggca accgctggtg catccatccg atggagggct gggacgccaa
     2341 ccgcgacggc tcgcccagcg agttgacgct gcggcggtgg tggcactttg ggctctccgg
     2401 cgcgaagctg atctggggcg gcgaagcggc ggcggtgcag cccgatgggc gggcgaatcc
     2461 gaaccagacg ctggcggtgc catcgaacaa ggccggcctg gcggcgctct ggaacgagtg
     2521 cctggccgcg catcggcggg cgtttggccc aaatgcgact gacgacctgg tcgtcggcct
     2581 gcagctcacg cattccggcc gcttttgccg cccgaactcc aagcagcttg agccgcgaat
     2641 cgcgtatcac catccgctgc tcgatgcgaa gttcggcatc cggcccgacg acaatgcggt
     2701 cgtctggagc gacgacgact tggagcggct gatcgacagt tatgttgttg cggctggatt
     2761 ggcccgcgag gtggggtatc agttcgtcga tatcaaggcc tgtcacgggt atctgctgca
     2821 cgagttcctc tcggcccgca cgcggccggg gcgcttcggc ggcgatctcg acggccgcac
     2881 ccggctgctg ttcacgatca tcagccgcgt gcgagccgca ttcccggatc tgggcgtttt
     2941 cgtgcgggtc agcatcttcg atacgctccc ctacaagacg agccgcgaag ttggccagcc
     3001 gatggagtat cagcagctcc tgccgtacca attcggtttc ggcgcccagg ccgacgatcc
     3061 gcggcagatc gacctggtcg agggcatcga gctgctccag cgcctgaagg cggcgggcgt
     3121 gttagcggcg aacatcagtt gcggcagccc gtactacaac ccgcacatca tgcggccggc
     3181 gatttttccg cccagcgacg gctatcagcc gccggaggac ccgctggtgg gcgtcgtccg
     3241 gcagattcac gccgcccgcg cgtgcaaagc ggccgtgccg gagcttgcca tggtgggcac
     3301 cggctacagc tacctgcagg actatctgcc acacgtggcc caggcggtga tccgcgccgg
     3361 ctggatcgac ctggtcggcc tcggccggat ggtgctctcg tatcccgaaa tgcccgccga
     3421 cacgctccag cgcggcacca ccgagcggaa gaaggtctgc cgcacgttca gcgactgcac
     3481 cactgccccc cgcaacggcc tggccagcgg ctgctatccg ctggatccgt actacaaaga
     3541 gcggccggag gcggaggagc tgaagcgggt gaagcaggag atcaaaggct aatccccgcg
     3601 gcgccggcag aaaaggcagc cacgccccag cggcaggaca acggcgagga ttccggagga
     3661 gaatgttgat cgttgtaagt cagttccctg gttgcccttg cgcagccagg gacttcgcaa
     3721 aatgcgaagt ccgccttgac agcttcgcat tttgcgaaca tccttcaccg tcctgacgaa
     3781 gtagcgttca gatgcacgtc atcgctagag caaccctgga tgcattcggc aggaaacaca
     3841 gggcggccaa ggagtggctg gaaaactggt ggcaaacggc agtcagggcc aactggagga
     3901 acctggatga tgtcaggcgc gtctatcagt ccacggatca ggtcggaggt tgtttggtat
     3961 tcgattgcaa gggcaacaac taccgactga tcgtccgcgt ttcctatgcg aacaagtaca
     4021 ctcgcggaac cctgttcgta aagcacctac tgacgcacgc ggaatacaac aagaaccgct
     4081 ggaaagactg ctgcgaatga ccacggcaac cctagaccgg atcgtcgcga gccgctcgta
     4141 cgagacgctt gtaagccgat tcccgccgcg tccaattcgc gagccagggc tcctgaagca
     4201 gacgtatgcg gtgatcgacc gcctgatggc gattccccac ccgtctccat tccacttgga
     4261 gtttctggaa cttctttcga cattggtgga ggattacgag tcggtccact acccaactcc
     4321 agtcacttcc acggagagcc tcctggcaca tctgattgag tcccgcgggg tgacacagac
     4381 gcaggttgcc aaggaaaccg gaatcagtcc ttcgatcatc tcggatgtgc ttgcgggacg
     4441 tcgctctctg agccttccag ctatcgtcaa gctgtcgcag tacttccgtc ttgatccagg
     4501 ggccttcttg cgcgtggctc gcgatatgtg accggggagt tgtggaagag tttagcgcgc
     4561 acctgcggga gaaaaaatgg aaaagtttcg cgctggatcg ccgcacggga gcctgccgga
     4621 gtcgccaggc gcttgccttg cgaccgcgcc gtcggatagg ctgcgcaagg tttcattctg
     4681 agacgttacg ggggttagcc atgtcgaagt tctgcatcca tccgctgctc gtggtgtcgt
     4741 gcctgctggt tatccaggca agcgggtcgt ccgcggctga ccaggattcc ggcgagcagc
     4801 tcctgccggc gaatccggcg atgtggctca actcgccccc gttgacgagc gacacgctca
     4861 aaggcaaagg ggtcgtgctt tggttttatg aggagcagtg cccccgctgc cgcgagcgct
     4921 ggccggggat gtacgacgtg gccaagaagt ttgacgggaa gccggtcgtg tttatcgccg
     4981 tgaattccgg caatccgcgc gacgaggtgg agcaatacgc caagggggtc aagcttccct
     5041 ggccggtgat cgtcgatcaa agccgcgaat tcgagaagca gtggcttgat agcgagatca
     5101 gcctgcagaa cattcatcag ctcgcgctca ttctgcccga tggccgcaaa cagatggcca
     5161 actggaacga tttcgaaggg gcggtgcaaa aggccgccga aggtgcgacc tggaagatcg
     5221 atccccagac gatccccgcg gccttccagc ccacgtggcg gctggtggaa atgggcaagt
     5281 acgaccaggc ggcagcgatg gtgaaaaagg ggctcaccac cagcaacgcc gaggtgaagg
     5341 aggcagcgac ccgcgtccac gaattcgtgc agcaggaatt gaagaagtca gccgagcggg
     5401 ccgcgaagct gcgccaggaa ggcgacacct ggaacgccta tcaggcctac cgggagatcg
     5461 cggcgtcgtt tgccggctac gagctgccgc tggaagtctc cgcggctcaa aaggagctgg
     5521 cagccgacga gaaggtcaag cagcagctcg acgcggcgcg ggcgctggaa tcgatcaaga
     5581 aatcgttccc caccgcgcgg accgacatcg ctaggaagcg catcgtccgc cgcctggagc
     5641 agctcgtcga gctgttcccc gacagcgagg cagcggtcga ggccaagtcg attttggaac
     5701 aggcggcgga gcagcagcag tagcagggct ttgccagagt caggggccag gggccaggag
     5761 ccaggggact ggcgaaaggt cgaacctcct gcgctgccct atactccgta ctgagtactc
     5821 agtacacagt acctagcaac gagcaagaat cggggatctt tcccgactcc tggccccaga
     5881 ctcctgaccc ctggccccat gccccgctat acgcccgcga ccatcgagcc caagtggcaa
     5941 cgctactggg acgagcacaa gaccttcaaa acgccggaaa tgccgccggc tggcagcgag
     6001 aagctgtacg tgctcgacat gttcccgtat cccagcggca gcgggctgca cgtgggacat
     6061 cccgagggct acaccgcgac cgacatcgtc tgccgcttcg cccgcatgaa cggcaagtgc
     6121 gtgctgcacc cgatgggctt cgacgccttc ggcctgcccg cggaagagca cgccatccgc
     6181 accggccagc acccgcgcgt caacaccgag cagaacatcg ccacgttcac gcggcagctc
     6241 aagatgctcg gcttcagcta cgactgggac cgcaatctgg cgacgaccga cgttgagtat
     6301 tttcgctgga cgcagtggat cttcctggtg ctgttcgaca cctggttcga cgccgaccag
     6361 caaaagagcc ggccgatcag cgagctgccg atcccgccgg ccgtaatatc gaaaggcgat
     6421 atcgcgattc gtaaatatca ggacgagcat cgattggcct atcagtcgga cgccctcgtg
     6481 aattggtgcc cggagctggg tacggtgctg gccaacgagg aagtcatcga cggcaaaagc
     6541 gaacgcggca gccatcccgt cgtccgtatg ccgctcaagc agtggatgct aagaatcacc
     6601 gcctacgccg accggctgga gaaagacctg gagccgctgg attggtcccc cggcatcaag
     6661 aagctgcagc gcgactggat tgggcgcagt gtcggtgcgg aagtggactt ctacgtgggg
     6721 cgggcgtctc gcctgcccga gaagacagaa cacagaggac agaggacaga ggacagcgag
     6781 ttcgacgcct ggaaagcctc ccgcgccaaa tccggcttgc ccaaaaagac cggcgacgac
     6841 gttctccgca tctacaccac gcgccccgat acgctcttcg gcgcgaccta tatggtcatc
     6901 gcgccggagc atcccttcgt cgcccggctg acgacgcccg atcaaaaggc cgccgtcgaa
     6961 gcctacgtcc agaccgccgc tcgcaagagc gaccgcgaac ggcaggaaga aacgaagaag
     7021 aaaacgggcg tcttcaccgg ctcgtatgcg atcaaccccg tcaacggcca gcccgtgccc
     7081 gtctgggtcg ccgactacgt cctctggggc tacggcaccg gcgcgatcat ggccgtcccg
     7141 gcgcacgacg aacgtgactt tgactttgcg atgcaatttg ggttgaagat tgttccggtg
     7201 attgaccccg gtgacatttc cgacgacgac atctatccgg agctgacgca agtactgagt
     7261 actcagtact cagtactcag tactgacgcc gagtccgccg acaatccaaa atccaaaatc
     7321 caaaatccaa aatctcctcc cgcgcgtcaa cgtgttcttg ccggccaggc ctgcttcgcc
     7381 ggcgaaggcg tctcaatcaa ctccggcccc tacaacggcc gcacaaccgc cgatttcaag
     7441 cagcgcatca ccgccgacct ggacgctggg ggcctcggcc gcgaggcggt caactacaag
     7501 ctccgcgact ggctcttcag ccggcagcgg ttttggggcg agcccttccc gatcctgcac
     7561 gagttggacg ccagcggcaa tccgaccggc ctgatccgcg ccgtcccgga aaaagacctg
     7621 cccgtcgatc tgccgcatct tgacgacttc aaaccagccg ccgccggcgc gagccccgcg
     7681 ccgccgctgg ctaaagcgcc gaaggagtgg ctggagccga cgattgccgg caaacgctac
     7741 cgccgcgaaa ccaacacgat gccccagtgg gccggctcgt gctggtactt cctgcggttc
     7801 atcgacccga aaaaccgcca gcggttcctc gatccggcca aagagaaagc ctggatgccg
     7861 atcgacctct acatcggcgg cgccgagcac gccgtcctgc acctcttgta ttcccgcttc
     7921 tggcacaaag tgctcttcga ccgcggccat gtcagcacgg ccgagccgtt ccagaagctg
     7981 gtcaaccagg gcatgatcct gggtgaaaac ggcgagaaga tgtccaaggc tcgcggcaac
     8041 gtcatcaatc ccgatgacgt ggtgcgcgat tacggggccg attcgctccg cctgttcgaa
     8101 atgttcatgg gaccgctcga agcggtgaag ccgtggagca cgcagggcgt cgtcggcgtc
     8161 cgcggctttc tggaccgagt ctggcggctg atggttgatg acaaggcgga cgagctggcg
     8221 ctgaatgcgg ccgtgcagaa ggtcgagcca acgaacgagc agctccgcac actgcacaag
     8281 acgatcaagc aggtgaccag cgacgtccag cggctggagt tcaacacggc gatcgcccgg
     8341 atgatggagt ttacgaactt cttcaccaag gagtcggtcc gcccgcactc cgtgatggag
     8401 cagttcgtgt tgatcctctc gccgttcgcc ccgcacatcg ccgaagagct gtggcagctc
     8461 ctcggccaca cccagacgct ggcctacgag ccctggccca agttcgacga cgccctgacc
     8521 aaggatgccg aagtcgaaat ccccgtccag atcctcggca agctgcggag caaggtgatc
     8581 gtccccgccg gcagcgacca ggccaccatc atcgccgccg cccgcgccga cagccgcatc
     8641 atcgagctcc tcaccggcaa agaggtcgtc aagaccatcg tcgttccggg gaagctggtg
     8701 aactttgtgg taaagtagca gtggcatgaa cagccttggc gtgctaagga ggcaaggtaa
     8761 tgactcaagc aactcagcaa gtattgcagg ccgcaatggc gctttccgat gacgagcgcg
     8821 ccgaacttat cgttcatctg gaggacacca tggcgggatt cgccacacca gaagtcgccg
     8881 cagcatggga cgcagagatc gccaagcgtc tcatggaaat cgatgacgga acggtggagt
     8941 tgagtccagc cgaggaagtc catcgtcgat tgggcgagaa gtatggcttc tttgccgatt
     9001 ggatatcatc ccgaggccac ggaagagctg tctgaggcgc tcgactggta cgcgcagcga
     9061 gatccacgca tcgccgatcg attcggtcgc ctgtatctcc gaaagctcaa cgaagccgcc
     9121 aagaggccgg ggcgctgggc cagacatcgt gatggcactc ggcacgtctt gctgtctcgc
     9181 tttccgtacc agttgatcgt tcgtgagaaa cgtggcgtgc tcgaactaat tgccgttgca
     9241 caaaccagcc gtcggcctgg ctactggcgc catcgtctca agtaattcat ttttcccaat
     9301 caacgtgccg aatgcctgac gacctcatca ccactcccgc ccagctcgaa gccctctacg
     9361 gccagccggg gcagctcgtg cgacagatgc agctttccaa gctcgagccg cagtcgatgg
     9421 cgttcatcgc gcaggcgccg ttcgcggtgc tggcgacagc cgggggcggt gggttcgcgg
     9481 acgtctcgcc caagggggat gcgccggggt ttattcaggt cgtggacgaa acgacgctgc
     9541 acctgcccga tcggctgggg aacaaccggg tcgaatcgct gaagcacatt ctcgagaacc
     9601 cccgcgtcgg cctgctgttc tttgtgcccg gccgcgaaga gacgctccgc gtccacggca
     9661 cggcccggat tcgcatcgat ccggagctgc tc
//
LOCUS       DASUGB010000439         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1718167, whole genome shotgun sequence.
ACCESSION   DASUGB010000439 DASUGB010000000
VERSION     DASUGB010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718167"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1123)
                     /gene="xerD"
                     /locus_tag="VFV87_08520"
     CDS             complement(176..1123)
                     /gene="xerD"
                     /locus_tag="VFV87_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5103839.1"
                     /translation="MKPLKPKLLAPAAARNPAGGQAERWAAACLDYLRTECRLADNTI
                     AAYRRDLNRFCAWLGGRLAAKMGVRELSEYMGWLQTQQLAPASIARHIVALKIFYRYL
                     QLEGAVRDNPAELLGTQKRWQYIPEVLSPALVERLLAAPQRYDAFWRRDRAMLELLYA
                     TGCRVSEVSQLKLADLHLAERHCLAHGKGAKDRLTPLGDAAVAAIQEYLNEERPRLAK
                     AAQSPPPWVLLSRGGRRLRREAIWQLTKGYARRSGVSAETSPHTLRHSFATHLLAGGA
                     DLRQVQELLGHASIATTQIYTHVDQSRLKRVHQQFHPRA"
     gene            complement(1390..2787)
                     /locus_tag="VFV87_08525"
     CDS             complement(1390..2787)
                     /locus_tag="VFV87_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103840.1"
                     /translation="MHRRRFLVASSLGFAGFAFGRSAPSAASQTKSPTQGTAKSTILF
                     FLCGGASHLDLWDLKPDAPAEYRGPFQPIATTAPGVRLCEHLPLLAGQAHHLALVNSV
                     GATVNTNDHHAGYYYHLTGHVADRSFQTLGNNRTPFADDWPFMGSVVAARRPPHEYLP
                     SAITLPHMPSKAPYTRPGQFAARLGVEHDPLYLHGSLEEPLRFEAPALVLEGEITADR
                     LTQRRSLLEEIDAARHDFDRYVRVRTWREHQERAASLLLSSRTTAAFEVAGEPEKVRE
                     RYGQTVNGMSLLLARRLVEAGVPFITVFWKENEKKLAKKCASAGGWDTHGNNFNCLKE
                     DLLPEFDRAFSALIEDLAQRGLLDQTLLLVTSEMGRTPKIGDPRSGGAGGAGRDHWTH
                     CLTDVLAGGGIRGGQVYGASDRLGEYPAADPVTPADITKTVYRAMGITDLTAVGPQNR
                     PYHLLEDGQVIERLF"
BASE COUNT          463 a          963 c          953 g          465 t
ORIGIN      
        1 cgatctgccg ggatcgcggg aaattcaaat ggcagacaac ctcttcgccc ggtacgaagt
       61 cggcagcgaa tcgccactgc agccggtgac ggttaccatc acggcagctc aggccgcagc
      121 gctgctcgga tcaggcggcg ctggcccacc gccgccgtga ttgccggccg gcgctctagg
      181 cccgcggatg gaactgctgg tggacgcgct tcagccgcga ttgatcgacg tgtgtgtaaa
      241 tctgcgtcgt ggcgatgctc gcatgtccca gcagctcctg cacctgccgc agatcggcgc
      301 cgccggccag caggtgcgtg gcaaagctgt gccgcagcgt gtgcgggctg gtctcggcgc
      361 tcacgccgga gcggcgggca tagcccttgg tgagctgcca gatcgcctcg cgccgcagcc
      421 gcctgccgcc gcgcgacagc aggacccacg gcggcggaga ctgggcggct ttcgccagcc
      481 gcggccgctc ctcgttcagg tattcctgaa tggccgcgac cgcagcgtcg cccagcggcg
      541 tcaggcggtc cttcgctccc ttgccgtgcg ccaggcaatg gcgctcggcc aggtgcaggt
      601 ccgccagctt gagctgcgaa acctccgaca cgcggcagcc ggtggcatag agcagctcca
      661 gcatcgcccg gtcgcggcgc cagaaggcat cgtaccgctg cggggcggcg aggagccttt
      721 cgaccagcgc gggcgagaga acttccggaa tgtattgcca gcgcttctgc gtgccgagga
      781 gttccgcggg attgtcgcgc actgcgcctt cgagctgcaa atagcgatag aagatcttga
      841 gcgcgacgat atgccgggcg atgctggccg gcgcgagctg ctgcgtttgc agccagccca
      901 tgtactccga cagctcgcgc acgcccatct tcgccgccag ccgcccgccc agccacgcgc
      961 aaaaccggtt caggtcgcgg cgataggcgg cgatcgtgtt gtccgccagg cggcactccg
     1021 tccgcaggta gtcgaggcag gccgccgccc atcgctcggc ttgcccgccg gcaggattcc
     1081 gcgccgcagc cggcgccagc agcttgggtt tgagcggctt catcgctggc ctctcttaac
     1141 cacatcggca gaggcagcgt cagaaaagca gagcgatcgt ggcagaagcc gccgcggccc
     1201 gatgcgcccc aatcgttaga ttcatcacag ggcgcgaagt gcgacccgcg acaagccaac
     1261 cgcccctgcc cggcagcaga ttccctgccc ggcagcagat tcactgcccg gcggcagatt
     1321 cactgcgccg cggcagattc gctgcgccgc ggcagattcc ctgcccggcg gcagattccc
     1381 tgcgcggaat cagaatagac gctcgataac ctggccatcc tcgagcaaat gataaggccg
     1441 gttctgcgga ccgacggccg tcagatccgt gatgcccatc gcgcgataga cggtcttggt
     1501 gatgtcggcc ggcgtgacgg gatcggcggc ggggtactcg cccaagcggt cgctggcgcc
     1561 ataaacctgg ccgccgcgaa tgccgccgcc ggcgagcacg tcggtcaggc aatgagtcca
     1621 gtgatcgcgg cccgcgccgc cggcgccgcc ggaccgcggg tcgccgatct tcggcgtgcg
     1681 ccccatttcg ctggtgacca ggaggagcgt ctgatcgagc agcccccgct gggccagatc
     1741 ctcgatcagc gcggaaaacg cccgatcgaa ctccggcagc aggtcttcct tcagacagtt
     1801 gaagttgttg ccgtgcgtgt cccagccgcc ggcgctagcg cacttcttcg cgagtttctt
     1861 ctcattttcc ttccagaaga ccgtaatgaa gggcacgccg gcctcgacga gacgccgcgc
     1921 caagaggagg ctcatgccgt tgacggtctg gccgtagcgc tcgcggacct tctccggttc
     1981 gccagcgacc tcgaaagcgg ccgtcgtgcg cgacgacagg agcagcgaag cggcccgctc
     2041 ctggtgctcg cgccacgtgc gcacgcggac gtagcggtca aaatcgtgac gagcggcgtc
     2101 gatttcctcc agcagcgagc gccgctgcgt cagccggtcg gccgtgatct cgccttcgag
     2161 gaccagtgcg ggcgcctcga atcggagagg ctcctccaag ctgccgtgca ggtacagcgg
     2221 atcgtgctcg acgcccagcc ttgcggcgaa ctgcccgggc cgcgtgtacg gggctttgct
     2281 gggcatgtgc ggcagcgtga tcgcgctggg caagtattcg tgcggcggcc gcctggcggc
     2341 caccacgctt cccatgaagg gccaatcgtc cgcgaagggc gtgcggttgt tccccagcgt
     2401 ctgaaacgag cgatccgcca cgtggcccgt cagatggtag tagtagccgg catgatggtc
     2461 gttcgtgttg acggtcgcac ccacgctgtt gaccagcgcc aaatgatgcg cctgcccggc
     2521 cagcagcggc aaatgctcgc acagccgcac gccgggcgca gttgtggcga tcggctgaaa
     2581 cgggccgcga tattcggccg gggcgtcggg cttgagatcc cacaggtcga ggtgcgatgc
     2641 cccgccgcac agaaagaaca ggatcgtcga ttttgcggtt ccttgagtgg gcgatttggt
     2701 ttgactggcg gcactcggcg cgcttcgccc aaacgcgaaa cccgcgaagc ctaaactcga
     2761 cgcgacgaga aaccgtctgc gatgcatgcg ggtgttcctt acgcggtggg ccttatctac
     2821 ctggcgctgc aaccggccac ggct
//
LOCUS       DASUGB010000440         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_884599, whole genome shotgun sequence.
ACCESSION   DASUGB010000440 DASUGB010000000
VERSION     DASUGB010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884599"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFV87_08530"
     CDS             complement(<1..449)
                     /locus_tag="VFV87_08530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5103841.1"
                     /translation="MKISATIITLNEELNIARAIESLRCCDEIVVVDSNSTDRTCEIA
                     ANLGARVIDNPFPGYAKQKNFASEQASNDWILSIDADEALSEALEAEIWHIKKHGPEF
                     DGYTFPRLAQYLGRWILHSGWHPDRKVRLFNRTKAEWVGDYVHESVRV"
     gene            complement(457..1401)
                     /locus_tag="VFV87_08535"
     CDS             complement(457..1401)
                     /locus_tag="VFV87_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5103842.1"
                     /translation="MSSVQPAVFPYATVVQGRRRLWLHILLLCLTLLTTTVMGARLVH
                     NFAHNVPPFSDDDLDAFARLLSNPALLLDGLPFSLTLMTILMAHEMGHYIACIYYRID
                     ASLPYFLPAPTLIGTLGAFIRIRSPISTRRELFDVGVAGPIAGFLVLLPALFIGVAYS
                     KVIPDINLRGEIYFGVPLLQSLAQQLLFPGTPAADLYLHPIGRAAWVGLLATALNLLP
                     IGQLDGGHILYAFFGRWHRTLTHVFIALLIPMGILYSLSWIVWAILLFVFARKHPSVH
                     DRLPLGTARAALAILSLLIFIAAFRLSPFRMLDIPTGQ"
     gene            complement(1418..1684)
                     /locus_tag="VFV87_08540"
     CDS             complement(1418..1684)
                     /locus_tag="VFV87_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5103843.1"
                     /translation="MIKLDIINEVVNKTGITKTKAEMAVETVFESMKRALSSGERIEL
                     RGFGIFNVRPRKTGIGRNPRTGAEVAIPPGKAVRFKPGKELQAL"
BASE COUNT          424 a          518 c          765 g          401 t
ORIGIN      
        1 acgcggacgg attcgtggac atagtcgccg acccattcgg ccttggtgcg attgaagagg
       61 cggaccttgc gatcgggatg ccagccgctg tgcaaaatcc agcgccctag atattgagcg
      121 aggcgaggga aagtgtagcc atcgaactcg gggccgtgtt ttttgatgtg ccagatttcg
      181 gcttcaagcg cttcgctgag ggcttcgtcg gcgtcgatgg agagaatcca atcgttgctg
      241 gcctgttcgg aggcgaagtt tttttgtttg gcgtagccgg gaaagggatt gtcgatgacg
      301 cgggcgccga ggttggcggc gatctcgcag gtgcggtcgg tggagttgga gtcgacgacg
      361 acgatttcat cgcagcagcg caggctttcg atggcgcggg cgatgttgag ctcttcgttc
      421 agcgtgatga tggtggcgga gattttcatc gtgcgcttac tgccccgtgg ggatgtcgag
      481 catgcggaag ggagacagcc tgaatgccgc gatgaagatg agcaggctca ggatggcaag
      541 cgcggcgcgg gccgtcccga ggggcagcct gtcatgcacg ctgggatgct tgcgggcgaa
      601 gacaaacagc aggattgccc acacgatcca gcttaacgag tagagaattc ccatggggat
      661 cagcagcgcg atgaatacgt gggtgagcgt gcgatgccag cgcccgaaga aggcatagag
      721 aatgtggccg ccgtcgagct gtccgatggg gagaaggttg agcgcggtag cgaggaggcc
      781 gacccatgcg gcgcgtccga tgggatggag gtagaggtcg gcggcgggcg tcccgggaaa
      841 gagcagttgc tgggcaaggc tctgcagcag cggcacgccg aaatagatct cgccgcgaag
      901 attgatgtcg gggatgactt tcgaataggc gacgccgatg aagagcgcgg gaagcagaac
      961 gaggaacccg gcgatggggc cggcgacgcc gacgtcgaac agttcgcggc gtgtggaaat
     1021 cggcgagcgg atgcggatga aggcgccgag ggtgccgatc aaggtaggag ccggcaggaa
     1081 gtacgggagc gaggcgtcga tccggtagta gatgcaggcg atgtagtggc ccatctcgtg
     1141 agccatgagt atggtcatca gcgtgaggct gaagggaagg ccgtccagca gcagcgccgg
     1201 gttggaaagg agacgcgcga aggcgtcgag gtcgtcatcg gagaagggcg gaacgttgtg
     1261 cgcgaagttg tgcaccaagc gcgcacccat caccgtggtg gtgagcaggg tgaggcacag
     1321 caacagaatg tgaagccaga ggcggcgccg gccctggaca accgtggcgt acggaaaaac
     1381 ggccggctgg acggaagaca cctgaaatgc cagcggtcta gagggcctgg agttccttgc
     1441 caggtttgaa gcgcactgct ttgcccggag gaatggctac ctcggctccg gtgcgcgggt
     1501 tccggccaat gccagtctta cgcgggcgaa cgttgaagat gccgaagccg cgcagctcga
     1561 tgcgctcgcc ggagctaagg gcacgcttca tgctttcgaa gactgtttcg acagccattt
     1621 cggccttggt cttggtgatg cccgtcttgt tgacgacttc gttgattatg tcgagtttga
     1681 tcaaatcgtc ctccccgctg agcggtcgcg cggccaaatc gcgcgctgcc cacaaccaaa
     1741 tgatatgatt ggctttaacc ctttgtcaag gaaccggggt cgaggagaag tcaagctctt
     1801 ggacggacga gaatttcgac gctgctgcgg acagtttgcc acaggggtcg cggtggcgtg
     1861 cgttttcggg aaagacggcg ggccacacgg gttgacggtg aacagtttta gttcggtgtc
     1921 gctggaccct ccattgattt tgatttgcat cgggcacgat tcgggcgtgc tcgagatgtt
     1981 ccatgagcag cggcggttcg gcttgagctt tctgcgggcc gaacaacggg atctttccaa
     2041 tcagttcgca atgcgggggc aaaaccggtt cgacggagtt gcctggcata cgggggaatc
     2101 gccggttc
//
LOCUS       DASUGB010000441         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_745137, whole genome shotgun sequence.
ACCESSION   DASUGB010000441 DASUGB010000000
VERSION     DASUGB010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745137"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFV87_08545"
     CDS             complement(<1..591)
                     /locus_tag="VFV87_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5103844.1"
                     /translation="MSQPTVAPGLQELLANAQLPALPQSAIRLLELSQNPDNGPAEFA
                     KPIESDPGLTGQVLKFVNSSYFGFTREISSVKLAITLVGIRTIKNFALWSAVFSLMPN
                     PKCGPFDLKSLWQDSLRRGLFARAFGKLIGLKEAEELFASALLQDMAIPLLAKEQPQR
                     YLRLLEQRTLGESRLSDLEREEFGWTHADAAGYMARK"
     gene            1420..>3007
                     /locus_tag="VFV87_08550"
     CDS             1420..>3007
                     /locus_tag="VFV87_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103845.1"
                     /translation="MRLTLRTMLAYLDDVLDPADAEVLGKKIEESEFASTLVKRIREI
                     GKKVRMDAPKLDGKGMGNDANTVAEYLDSTLPQDRVGDLERICLESDRHLSEVAACHQ
                     ILTLVLGKPADVPEGVREKIYLLGQPDHAPATTKKVKSPPIPTTNGKPHAHPAPEVPD
                     YLRSGQRSSVLPFLAAVAAAFLIGAVVLRAMGPFNGSHPVMRWFGADTSVAEASKNAE
                     TPGELAPEPQESPKNATSSTPSPKDPVESTGDTEPPAPAPSDTDTPVPPEPVEPPMAP
                     RPEVTLPEVPEEAPKPETTPKPETAPEVETTPKPPVPMPPPLAKPADPAIAMDVGRYV
                     SDEQIMAALNPEDGLWYIKPTRAVLAAGERLLVLPTYRPQIALPSGVQLTFASESAIQ
                     MEEPSESGTSRLTVDYGKFVAVTFGAAGAQIELNLAGIEGTVTLVDADSSLAVKVARW
                     HPPGTNPEVGPGMPVLELFTSHGRVTWQQTDQKKVDIPANHVHIYVGAEPPETHGPFY
                     APEWIDPRSTSGIDRETSLVL"
BASE COUNT          584 a          920 c          948 g          555 t
ORIGIN      
        1 cttgcgggcc atatagccgg cggcatcggc gtgcgtccag ccgaattctt cgcgctccag
       61 gtcgctcaag cgcgattcgc cgagcgtccg ctgctccaag aggcgcagat accgctgcgg
      121 ctgctccttg gccaggagcg ggatcgccat gtcctgcaac agggcgctgg caaacagctc
      181 ttcggcctcc ttcagtccga tcagcttccc gaacgcccgg gcgaagaggc cgcggcggag
      241 cgaatcctgc cacaggctct tgagatcgaa cggcccgcac ttggggttgg gcatcaggct
      301 gaagaccgcg ctccacaggg cgaagttctt gatcgtgcgg atgccgacca gtgtgatcgc
      361 cagcttgacg ctcgaaatct cgcgcgtgaa tccgaagtac gacgaattca cgaacttgag
      421 cacctggccc gtcaggccag ggtcggattc gatcggcttg gcgaactcgg ccgggccgtt
      481 atccgggttt tgcgatagtt ccagcaggcg gatcgcactt tgcggcagtg cgggcaattg
      541 ggcgttggcg agcagttcct gaagtccggg agcgactgtg ggttgtgaca tcggggcgat
      601 cctccgcggc cggttcgggg aatgagcttg cgcacctgcg cctggcgcta ggttgcggcg
      661 acgaagggac ttgcgcgaca ctgaaaccta ggacacgctt gacagccggg caaattggcg
      721 gcctgagggg cgtctggtgc ggacattcca gaattgacgg gctggacgaa ggcgcgaggg
      781 gtgatgtttc ccggtaacaa cacgtggcgt ggtgaataat ctacatgctc gcgggtgaaa
      841 gtcttctcgt attttgctgt aactaattgc cgctaaacaa cttgagacag cggcctgcct
      901 gggaaccctt cccagggcac tcgtcttgcc tggattgcct tcgcttcaaa aatctctgcg
      961 aaggttcagg catgctcaga agcgtattta taaggtccgt tccagtaatc acttacagct
     1021 ttgccgtcgc cctcatagtt gttacgcacg gcacgcttgt cgcctcgcca cgtgcgcgtg
     1081 ccgatggact ggcgacagcc agcgaggacc catggcggcg aaccgcaacg gggtgggaat
     1141 acgtgcaaac atgggacgag tcccgatttg cggcagcttc ggcatcgcag gttcgcgtcg
     1201 atagccaccc ggcggcactg gcgtttttcg agcttctagt catcggagcg gcatttttcc
     1261 tgtttccaac gcgaatcgcg caacttgctt cgccgcccag acttacatcg acgcctcgga
     1321 tcacgtgatg agtggcggtc cgaattcttg cacgctgaag ccgccagcca gccactaaca
     1381 aggatgggag aagttgttgg cttgtagcgg agaacggtca tgcgtctgac gttgcgaacg
     1441 atgctggcgt acctggacga tgtgctcgat ccagccgatg cggaagtgct tggcaagaaa
     1501 attgaggaaa gcgagtttgc ctccaccctc gtcaagcgga ttcgcgagat tggaaaaaag
     1561 gtgcggatgg acgcgcccaa gctcgacggc aaaggaatgg gcaacgacgc gaacaccgtc
     1621 gcggagtatc tcgacagcac cctgccgcag gatcgcgtcg gcgatctgga gcgaatctgc
     1681 ctggagtcgg accggcatct gtcggaggtc gccgcctgcc atcagatcct gacgctcgtc
     1741 ctcggcaagc cggcggatgt gcctgagggc gttcgcgaga agatctattt gctcggccaa
     1801 ccggatcatg ccccggcgac caccaagaag gtcaagtcgc cgcccattcc aacgacgaac
     1861 ggcaagccgc acgctcatcc cgcgccggag gttccagatt atctccgttc cggccagcgg
     1921 tcgagcgtct taccgttcct cgccgcggtg gccgccgcgt tcctgattgg cgcggtcgtt
     1981 ctgcgggcga tgggcccatt caacggctca catcccgtga tgcgttggtt cggcgccgat
     2041 acgtcggttg ccgaagcgtc gaagaatgcg gaaacacccg gtgaactggc gcccgagccg
     2101 caagaatcgc ccaagaatgc aacctcatcc acgccttcac ccaaagaccc ggtcgaatca
     2161 acgggcgaca ctgaaccgcc ggcgcctgct ccatccgata cagatacccc ggtgccgccg
     2221 gaacctgtcg aaccgccaat ggcgccgcgg cccgaagtca cgctcccgga agtccctgaa
     2281 gaggccccca agccagaaac cactcccaag ccggaaacgg cgcccgaagt ggaaacgacg
     2341 ccgaaaccgc cagtgccgat gccgcctccg ctcgcaaagc ctgccgatcc cgccatcgcg
     2401 atggatgtcg ggcgctacgt ctcggacgag cagatcatgg ccgctttgaa tccggaggac
     2461 ggcctgtggt acatcaagcc gacgcgagcc gtgctcgcag ccggcgagcg gttgctcgtg
     2521 ctcccaacgt accgtcccca gattgccctg ccctccggcg tgcagctcac gttcgccagt
     2581 gaatcggcca tccaaatgga agagccgagc gaatcgggca catcgcgcct gacggtcgat
     2641 tacggcaaat ttgtggctgt gacgttcggt gcagccgggg cgcagatcga gctgaacctg
     2701 gccggaatcg aagggacggt gacgcttgtc gatgccgatt cttcgttggc ggtaaaggtg
     2761 gctcgctggc atccgccggg gaccaatccg gaagtggggc cgggcatgcc ggttttggag
     2821 ctgttcacct cgcatggccg cgtgacgtgg cagcaaacgg atcaaaagaa ggtcgatatt
     2881 cccgccaacc atgtgcatat ttatgtgggc gcggagccgc ctgaaacgca cgggccgttc
     2941 tacgcgccgg agtggatcga tccgcggagt accagcggca tcgaccgcga gacgtcgctc
     3001 gtgctct
//
LOCUS       DASUGB010000442         4584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1162033, whole genome shotgun sequence.
ACCESSION   DASUGB010000442 DASUGB010000000
VERSION     DASUGB010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162033"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1515
                     /locus_tag="VFV87_08555"
     CDS             337..1515
                     /locus_tag="VFV87_08555"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX5103846.1"
                     /translation="MCCSTLRVLALAAGAFLLGGLAQAACQEKPELPLPAPLGAITMN
                     SVASQPFGKTKDGQEITVYTLINARALKARLINYGATLISVETPDKDGELANITLGFP
                     KLDGYLQRHPYFGSTVGRYGNRIAGGKYKLDGKEYTLATNNGPNHLHGGLKGFDAVVW
                     KAEPIKGPSAVGVKFTYTSQDGEEGYPGNLNVAVTYSLTNNNELKIEYEAKTDKPTVV
                     NLTNHTYWNLAGAGSGDILQHELTINAEQYLPIDDTSIPTGKPADVQGTPFDFTSPHA
                     IGQRIGELKKEPHKTKGYDHCYVLRGQEGKLTLAARVKDPSSGRIMEIFTTQPGIQLY
                     CGNFLDGSAGGGGYKQHDAFCLETQHYPDSPNQPQFPSTVLRPGETFKSTTVHKFLVE
                     "
     gene            1549..2019
                     /gene="bcp"
                     /locus_tag="VFV87_08560"
     CDS             1549..2019
                     /gene="bcp"
                     /locus_tag="VFV87_08560"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX5103847.1"
                     /translation="MSHWLEEGTKAPAFTLQADDGSKVKLADLKGSPVVLYFYPADDT
                     PGCTREACAFRDRSAELKQLGAKVFGISPDDVASHARFKSKFKLNFPLLADTDHKVAE
                     RFGAWREKNMYGNKKMGIVRSTFLIGADGVITKVWRSVKVDGHGEAVLAALKEL"
     gene            complement(2040..3566)
                     /locus_tag="VFV87_08565"
     CDS             complement(2040..3566)
                     /locus_tag="VFV87_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5103848.1"
                     /translation="MTALSTLLMVAALTAADDTVLVEFTSQNCTHCRAMQPVLERMAG
                     EGCPVQVIDVDQQPDVARQFRIEGVPTFVVLTRGQETGRVMGATSYEMLVQLYRGTAA
                     PVAAAGAAAGGQDAASLAAAQLPREAGPTPALRKDAAVSPMQSSASQYASSAAATSDN
                     PQAAALAASVRLKVEDATGYGFGTGTIIDVHDQEALVVTCGHLFRESQGKGRITVDLF
                     APGATQPIEGQLIAYNLDRDIALVSIRPGFALAPAVVAPPGTMVRPQDGAFTVGCDKG
                     AEASVRETQITAVNKYVGKPNFCAAGAPVDGRSGGGLFTSDGLLIGICNAADPADNEG
                     LYAGLASIHWQLDQIGQSEIYQRAAQANAATLADNGAAGSPAMPHSMPPSLPQREIEL
                     AAAPARATSPIPVTPAVYQSEAAPPAAAAPAGSDDTEIVFIVRSKSNPQERSEVYVVD
                     GATPDLMSRIAEAARTTGQRRAAQASARANAPAGHSIETAQHQRFGQPAIIRGQSGDY
                     "
     gene            4076..>4584
                     /locus_tag="VFV87_08570"
     CDS             4076..>4584
                     /locus_tag="VFV87_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103849.1"
                     /translation="MFRLELGSTRRYCDGLSRRSFLQVGVAGMGAVGLSQILRAKAAS
                     AAATGIKKDTSVILLWLDGGPGHMDLYDMKPEAPPEYRGIWRPIKTNVSGIEITELFP
                     LQAKIADKFSIVRSLHHDSGDHFTGGHWMLTGRNAGVSGANNAGKFPFFGSIATKVLG
                     PRQQGMPAN"
BASE COUNT          846 a         1566 c         1376 g          796 t
ORIGIN      
        1 cacccccaag ggacaaacca cgcccaggcc ggtgatgacg acttcgcgtg gagaggacat
       61 ggattcgacc aaacggcgac gccgcgctgg aggctcaatc tgctgcggca aacgagatcg
      121 ccggccgtcc catcctcgga aattccagcg tcagcctact tatcgaccag acgcgcggcc
      181 caaagcatgg tccgtaggca tcctccgcaa tgtaccatct tgctcagccg cgctaacagt
      241 tacaacttgc cttcagcatc cgcaagcgcg cttccctcgt tataatccgg tttttgaccc
      301 cgctcgcttg cgcactttct ccccgggagc tgctgcatgt gctgctcgac ccttcgcgtt
      361 ctcgcactcg ccgccggagc atttctgctg ggcggactgg cccaagcggc ctgccaggaa
      421 aagcccgagc tgccgcttcc cgccccatta ggcgccatca ccatgaacag cgtcgcatct
      481 cagccgttcg gcaagaccaa ggacggccag gaaatcacgg tctatacgct catcaatgcc
      541 cgtgcgctca aagcccggct gatcaattac ggcgcgacgc tcatcagcgt cgaaactccc
      601 gacaaggacg gcgagctggc gaatatcacg ctcgggtttc cgaagctcga cggctacttg
      661 cagcggcatc cctatttcgg ctcgaccgtc ggccgctatg gcaatcgcat cgccggcggc
      721 aaatacaagc tcgacggcaa ggagtacacg ctggcaacca acaacggccc caatcatctg
      781 cacggcgggc tgaagggctt cgatgcggtc gtctggaagg ccgagcccat caagggcccg
      841 agcgccgtcg gcgtgaaatt cacctacacc agccaggacg gcgaggaggg ctatccgggc
      901 aacctcaacg tggccgtgac ctactcgctc acgaacaaca acgagctgaa gatcgaatac
      961 gaagccaaga ccgacaagcc gacggtcgtc aacctcacga atcacacgta ctggaacctg
     1021 gccggagccg ggagcggcga catcctccag cacgagctga cgatcaatgc cgaacagtac
     1081 ctgcccatcg acgacacctc gattcccacc ggcaagccgg ccgacgtgca ggggacgccg
     1141 ttcgatttca cgtcgccgca tgcgatcggc cagcggatcg gcgagctgaa gaaggagccg
     1201 cacaagacga agggctacga ccattgctac gtcctccgcg gccaagaggg caagctgacg
     1261 ctggcggccc gggtgaagga cccctccagc ggacggatca tggagatctt caccacgcaa
     1321 ccaggcatac agctttactg cggcaacttc ctcgacggct cagccggagg gggaggttac
     1381 aaacagcacg acgccttctg cctagaaacc cagcactacc ccgattctcc caaccagccg
     1441 cagttcccca gcacggtgct gaggcccggt gagaccttta aatcgaccac cgtccataaa
     1501 ttcttggtcg agtaatcttt taccctccat ctataacctg gcggccccat gtcccattgg
     1561 ctcgaagagg gcacgaaggc ccccgcgttc acgctccaag ccgacgacgg ctcgaaagtg
     1621 aagctggctg acctcaaggg cagccccgtc gtcctctatt tctaccccgc cgacgacacg
     1681 cccggctgca cgcgcgaggc gtgtgcgttc cgcgaccgct cggcagaact caagcagctc
     1741 ggcgccaagg tcttcggcat cagccccgat gacgtggcca gccacgctag gttcaaatca
     1801 aagttcaagc tgaacttccc tctcctggct gacactgacc acaaggtcgc cgaaagattc
     1861 ggcgcctggc gcgaaaagaa catgtacggc aacaagaaaa tgggcatcgt ccgctcgacg
     1921 tttctcatcg gagcggacgg cgtcatcacg aaggtctggc gctccgtcaa ggtcgatgga
     1981 cacggcgaag ccgtcttggc ggcactgaag gagctgtaag tccctgtcgg tctctcgtct
     2041 caatagtcgc ccgattgccc gcgaatgatc gcgggctggc cgaagcgctg gtgctgggcg
     2101 gtttcgatgg agtgtccggc aggggcgtta gcccgcgcgg aggcctgcgc cgcgcggcgc
     2161 tggccggtcg tgcgtgcggc ttcggcgatg cgcgacatca ggtcgggcgt cgccccgtcg
     2221 acgacataga cctcgctccg ctcctgcgga ttgctcttgg accgcacgat aaacacgatc
     2281 tcggtgtcgt cgctgcctgc cggtgcggcg gccgccggcg gcgcggcttc ggactgatag
     2341 acggctggcg tcactggaat gggcgaagtt gctcgcgctg gcgccgcggc cagctcgatt
     2401 tcgcgctggg gcagcgacgg cggcatcgag tgcggcatgg cgggcgagcc cgctgcgcca
     2461 ttatccgcca gtgtcgccgc gttggcttgt gccgcacgtt gatagatctc ggactggccg
     2521 atctgatcga gctgccagtg gatcgaggcc agccccgcgt acaaaccctc gttgtcggcc
     2581 ggatcggctg cgttgcaaat gccaatcagc aggccgtccg acgtgaacag cccgccgccg
     2641 ctgcggccat cgaccggagc gccggcggca cagaaattcg gcttgccgac gtacttgttg
     2701 accgcggtaa tctgcgtttc gcgcacactc gcttcggctc ctttgtcgca gccgacggtg
     2761 aacgccccgt cttgcggccg caccatcgtg ccgggcggcg ccacgaccgc cggcgcgagg
     2821 gcgaagcccg gccggatgct caccagggca atatcgcggt cgaggttgta cgcgatgagc
     2881 tgtccctcga ttggctgcgt cgcgcccggc gcgaacagat cgacggtgat ccggcctttc
     2941 ccctgcgact cgcgaaacaa gtggccgcac gtgacgacga gcgcttcctg atcgtgaacg
     3001 tcgatgatgg ttcccgttcc aaacccgtag cctgtcgcgt cctcgacttt caatcgcacg
     3061 ctcgcggcca aggcggcggc ttgcggattg tcgctggtgg ctgccgcgct cgatgcatat
     3121 tgcgaagcgc tcgactgcat cggcgagact gccgcgtcct tgcgcagcgc tggcgtcggc
     3181 cccgcctcgc gtggcaactg cgcggccgcc aggctcgctg cgtcctgccc gcctgctgcc
     3241 gctcctgccg cggcaactgg cgccgccgtc ccgcgataaa gctgcacgag catctcatag
     3301 cttgtcgcgc ccatcacgcg gcccgtttcc tggccgcggg tgagcacgac gaacgtcggc
     3361 acgccctcga tgcggaactg ccgggccacg tccggctgct gatcgacgtc gatcacctgg
     3421 accgggcagc cttcgcccgc catgcgctcc agcacgggct gcatcgcccg gcagtgggtg
     3481 cagttctgcg acgtgaattc gaccagaacc gtgtcgtccg cagcggtcaa agccgccacc
     3541 atcagcagcg tagaaagagc ggtcatcgct tccctccatg gaatgtgcgg agaggaagcg
     3601 tggccggcgg gccgcgggag cttccttgcc gcgaatgtcc ctgatccttc aggggccggt
     3661 gcaagccggc atttgccagc gggtcgcacc gtgcgaaacc caatctgtcc ccaatgtgca
     3721 attcttacaa ggccaatttc acatgtttgc tgaaagcgca aaccgtacga cgggtttcca
     3781 acccgtcctg cacggacggg ttggaaaccc gtcctagagc acgcggcgcg ccaatcgcga
     3841 aactttaggg aatcctgtct gccacttgaa ttccgttgac ggcggtcatc cgcccggcct
     3901 agaataccga tcaacagagg tgaatgggtc gccctgtagc cgcactgccc ggagtgcggg
     3961 atcgctcgtt tgcccgcgtt ctgccgaacg cggctacgcc cacccaagaa ttgcccgcca
     4021 ccgccgccct gcttgcggcc gaatactgtt ttcccgcact tcgaggaccc cgcctatgtt
     4081 ccgcctcgag ctcggttcca cgcgccgcta ttgcgatggg ctttcgcgcc gcagcttcct
     4141 ccaggtcggc gttgccggca tgggggccgt cggcctgtcc cagatcctgc gggccaaagc
     4201 ggcctcggcc gctgccaccg gcattaaaaa ggacacctcg gtcattctcc tctggctcga
     4261 cggcggtccc ggccacatgg acctctacga catgaagccg gaggccccgc cggaatatcg
     4321 cggcatctgg cggccgatca agacaaacgt atcaggcatc gagatcaccg agctcttccc
     4381 gctgcaggcg aagattgccg ataagttctc catcgtccgc tcgctgcatc acgactcggg
     4441 cgaccacttc accggcggtc actggatgct caccggccgc aacgccggcg tcagcggcgc
     4501 caacaatgcg ggcaagttcc cgttctttgg gtcgatcgcg acgaaggtcc tcggtccccg
     4561 tcagcagggc atgccggcga atat
//
LOCUS       DASUGB010000443         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1300691, whole genome shotgun sequence.
ACCESSION   DASUGB010000443 DASUGB010000000
VERSION     DASUGB010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300691"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="VFV87_08575"
     CDS             <1..1955
                     /locus_tag="VFV87_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103850.1"
                     /translation="QQQQQQQQRSARSQQQQQQLNQLELNNDENRYQTQRAAQTQQPE
                     TPQERENRQVLNRLKELARRQHDLNERVKELQAALEAAQTELEKEEIRQQLKRLQDEQ
                     QQILQDTDELQNRMDAPENQERMAEDRQQLEQTREQVRRSAEALNQEQVTQAAASGTR
                     AEQEFEELRNEFRRRAAGRFNEEMREMREAAQELDQKQDEIRERLNQEAQPDQKSKSL
                     RDTGERERIAEELSQQRERLGDLQERMRETITEAEETEPLLSERLYDADRNLQQQSPE
                     RALQSAERSLRQGLVKDAREQQEPAGESIRQLREGIEQAAEAVLGDETEALRRARAEL
                     QELSRELNDEINRNNPGQPQERQEGDRQEGQPAQQGQPGERQPGQNQQERAGQPQQGQ
                     EPDGQKGQNGQKGQGQKGKAQPGQKGQGQKGQRGQPGQGGQPGENQEGQPMDQQAQDG
                     QPQEGQPQEGQQQDNRQPGQRGQSGQRGQPNQRGDDTARQPGQRGGQRRLTDDNGPTR
                     GGGNIEEFDRRISPLTGGEDFRDWSDRLRDVEEMVEDPDLRAEAARIRERARSIRAEA
                     QRHSAEPNWELAQQQVAGPLVTLTDRVAEELLRRTSQQAIVPLDRDPVPPQFSEKTRR
                     YYERLGSGNAQSSGNAQSSGGGKAGP"
     gene            1952..>3926
                     /locus_tag="VFV87_08580"
     CDS             1952..>3926
                     /locus_tag="VFV87_08580"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5103851.1"
                     /translation="MILADLVLGARQWLGVAIVLAAIALVAIVWAYARAGSQTWVRVL
                     AGLLKAAGIALLAICLVEPLFTGTRPRPGSNLLLVVADNSRSLQLADRGSRQSRGQIF
                     QARLAEKSPWLTRVAQDFDLRRYTFDTTLRPVKDFAELPYDGQASALQGSLTALAERF
                     KGQPIAGILLLTDGNATDLSDQLDWSQLPPVYPVVPGRGTELVDLSVARVSISQTNFE
                     AAPVTVTAEIEGQSVAGKKIVVRVLDEEQKELERRALVAPAGEEPLAQRFLIKPEKPG
                     VSFYTVQTFLEGEEAQRPGRTAEATLANNRRLATVDRGAGPYHVLYVAGRPNWEFKFI
                     SRAVAEDDEMRLFGLLRIAKKEPKFTFKGRQGERTNPLFRGFTNQDDPTAEQYNQPVL
                     LRVPPTKAEEQDLKGGFPKDAADLYQYHAVILDDVEAEFFTQDQLSLLQKFVSQRGGG
                     LLMLAGPHTFAEGNYQRTPVGEMLPVYLDRNIAPVSAGGYRLTLTREGWLQPWVRVRT
                     NEQDEERRLATMPAFKTVNRVNAIKPGATVLAQVESEGGEPRPALIVQPFGRGRTAAL
                     MVGDLWKWHLRREDYRESDHDKAWRQMIRWLVADVPQPVEVETRRASGTALPGVEIVV
                     RARDEQFLPLDNAGVTIKVNTPDQRNIELIAESS"
BASE COUNT          798 a         1256 c         1380 g          492 t
ORIGIN      
        1 gccagcagca acagcagcag cagcagcgca gcgcccgctc gcagcagcaa cagcagcagc
       61 tcaatcagct cgagctgaac aacgacgaga accgctatca gacgcagcgg gccgcgcaga
      121 cccagcagcc ggagacgccc caggagcgcg agaaccggca agtgctgaac cgcttgaagg
      181 agctggcccg ccgccagcac gacctcaacg agcgcgtcaa ggagctgcag gcggctttgg
      241 aagcggccca gaccgaactg gagaaggagg agatccgcca gcagctcaaa cggctgcaag
      301 acgagcagca gcagattctg caggataccg acgagctcca gaaccgcatg gacgcccccg
      361 agaatcaaga gcggatggcc gaagaccggc agcagctcga gcagacgcgc gagcaggtcc
      421 gccgctcggc cgaagccctc aatcaggagc aggtgacgca agccgccgcc agcggcacgc
      481 gggccgagca ggaattcgag gagctgcgga acgaattccg ccgccgggcc gccggccgct
      541 tcaacgagga gatgcgcgaa atgcgcgaag cggctcagga attggaccag aagcaggacg
      601 agattcgaga gcggctgaat caggaggcgc agccggatca gaagtcgaag tcgctccgcg
      661 acacgggcga gcgcgagcgg attgcagagg aactctcgca gcagcgcgag cggctgggcg
      721 acctgcagga gcggatgcgc gagacgatca ccgaggccga ggagacggag ccgctgctga
      781 gcgagcggct gtacgacgcc gatcgaaatc tccagcagca gtcgcccgag cgggcgctgc
      841 aatcggccga gcgctcgctt cgccagggcc tcgtgaaaga cgcccgcgag cagcaagagc
      901 ccgccggcga gagcattcgc caactgcgcg agggaatcga acaagctgcg gaggcggtcc
      961 tgggggacga aaccgaggcc ctgcgccgcg cccgggccga actgcaagag ttgtcgcgcg
     1021 agctgaacga cgagatcaat cgcaacaatc ccggtcagcc gcaagaaagg caggaaggcg
     1081 atcggcagga aggacagcca gctcagcagg gccagccggg cgagcgccag ccgggccaga
     1141 atcagcaaga gcgagcaggt cagccgcagc aaggccagga accggatggg cagaagggcc
     1201 aaaacggtca gaagggccag ggccaaaagg ggaaagccca gcccggccag aagggacaag
     1261 gtcaaaaagg ccagaggggt caacctggtc agggtggaca gcccggcgag aaccaggaag
     1321 gccagcccat ggaccagcag gctcaagatg gccagccgca agaaggtcag ccgcaagagg
     1381 gccagcaaca agacaatcgg cagcctggtc agcgcggcca aagcgggcag cgcggccagc
     1441 ccaatcagcg cggcgacgat accgcgcggc agccgggcca gcgcggcggg cagcgccgct
     1501 tgaccgacga caatggccct acgcgcggcg gcggcaatat cgaagagttc gaccggcgaa
     1561 tttcgccgct caccggcggc gaagacttcc gcgattggtc cgatcggctg cgcgacgtgg
     1621 aagagatggt cgaggatccg gacctgcggg ccgaggccgc gcggattcgc gagcgggccc
     1681 gctcgatccg cgcggaggcc cagcggcatt cggccgagcc gaactgggag ctggcgcagc
     1741 agcaggtcgc cggtccgctc gtcacgctga ccgaccgggt ggccgaggaa ttgctccgcc
     1801 gcacctcgca gcaagcgatc gtgccgctcg accgcgatcc ggtcccgccg cagttctcag
     1861 aaaagacccg ccgctattac gagcgcctcg gcagcggcaa cgcgcaaagc agcggcaacg
     1921 cgcaaagcag cggcggcggg aaggcgggtc catgattctg gccgatctgg tgctgggcgc
     1981 caggcagtgg ctcggggtcg cgatcgtact ggcagcgatc gccctcgtcg ccatcgtgtg
     2041 ggcttatgcc cgcgccggct cgcagacctg ggtccgcgtg ctggccggtt tgctcaaggc
     2101 ggcgggcatc gccctcctgg cgatctgcct tgtcgagccg ctcttcacgg gcacgcgccc
     2161 gcgacccggc agcaacctgc tgctcgtcgt cgccgacaac agccgcagcc tgcaactggc
     2221 cgatcgcggc agccggcagt cgcgcgggca gatcttccag gcccgcctgg cggagaaatc
     2281 gccttggctg acgcgcgtgg cgcaagattt cgacctgcgg cgctacacgt tcgacacgac
     2341 gctgcggccg gtgaaggatt tcgcggagtt gccctacgac ggccaggctt cggccctgca
     2401 gggctcgctc accgcgctgg ccgagcgctt caaagggcag ccgatcgccg gcattctgct
     2461 gttgacggat ggcaacgcga ccgacctcag cgaccagctc gactggtcgc agttgccgcc
     2521 ggtctatccg gtcgttcccg gccgcggtac cgagcttgtc gatctctccg tcgcccgcgt
     2581 gagcatcagc cagaccaact tcgaagcggc cccggtcacc gtcaccgcgg agatcgaagg
     2641 gcaatcggtt gcgggcaaaa agatcgtcgt ccgcgtgctc gatgaagagc aaaaggagct
     2701 ggagcgccgc gcgctggtcg ctccggccgg cgaagagccg ttggcccagc ggtttttgat
     2761 caagcccgag aagccgggcg tgagctttta caccgtgcag acattcctcg aaggcgaaga
     2821 ggctcaacgg cccggccgca cggcggaagc cacgctggcc aacaaccgcc ggctggccac
     2881 ggtcgatcgc ggggccgggc cgtatcacgt cctctatgtc gccggccggc cgaactggga
     2941 attcaagttc atcagccggg ccgttgctga ggacgatgag atgcggctgt ttggcctctt
     3001 gcggatcgcg aagaaggagc cgaagttcac gttcaagggg cgtcaggggg agcggaccaa
     3061 cccgctgttt cgcggattca ccaatcagga cgatccgacg gccgagcaat acaaccagcc
     3121 ggtgctgctc cgcgtgccgc cgaccaaagc cgaagagcag gacctcaagg ggggctttcc
     3181 caaggacgca gcggacctgt atcaatacca tgcggtgatc ctggacgacg tcgaagcgga
     3241 attcttcacg caggatcagc tttcgctgtt gcagaagttc gtcagccagc gcggcggcgg
     3301 cctcttgatg ctcgccgggc cgcacacgtt cgccgaggga aattaccagc gaacgccggt
     3361 gggcgagatg ttgcccgtct atctggaccg caatatcgct cccgtctcgg ccggcggcta
     3421 ccgcctgacg ctcacccgcg aaggctggct gcagccgtgg gtccgcgtgc ggaccaacga
     3481 gcaggacgaa gagcggcggc tggcaacgat gccggcgttc aagacggtga accgcgtgaa
     3541 tgcgatcaag ccgggggcca cggtgctggc ccaagtggag tcggagggtg gtgagccgcg
     3601 tcccgcattg atcgtgcagc cgttcggccg cgggcggact gctgccctga tggtaggcga
     3661 tctgtggaag tggcacctgc gccgcgagga ttacagggag agcgaccacg acaaggcctg
     3721 gcggcaaatg attcgctggc tggtggccga cgtgccgcag ccggtcgagg tggagacgcg
     3781 ccgcgccagc ggcaccgcgc tgccgggcgt ggagatcgtc gtgcgggctc gtgacgagca
     3841 gtttttgccg ctcgataacg ccggcgtgac gatcaaggta aacacgcccg accagcggaa
     3901 tattgagctg atcgcggagt cgagcc
//
LOCUS       DASUGB010000444         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_884929, whole genome shotgun sequence.
ACCESSION   DASUGB010000444 DASUGB010000000
VERSION     DASUGB010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884929"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VFV87_08585"
     CDS             complement(<1..1406)
                     /locus_tag="VFV87_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5103852.1"
                     /translation="MVRSFVAMLFVAAASQSQAEDPLAVEYFEREVRPILADHCQKCH
                     GATKQESDLRLDSREGVLQGGVSGPAIVAGDPAKSLLIAAIRHEGDIQMPPKGKLTEA
                     QLAAVTHWVKLGAPWPKDSGTAAKTADAARTHWAFQPVRDPSVPSVSDNDSPNPIDAF
                     IRAKLAEHKLSSSPSADRRTLIRRASYDLTGLPPTPDEVAAFEDDQDPKAYENLVERL
                     LAAPQYGEQWGRHWLDVARYSDTKGYVYAREQRFWLHAWVYRDWVVQALNDDLPYDRF
                     LLLQIAADQVAPDDPQSLAAMGFLTLGRRFLGVQRDIIDDRIDVVTRGTMGLTVGCAR
                     CHDHKYDPIPTRDYYSLYGVFQSCIERIEPLTAAALQDPAHQTYVAELKKRKAALAEG
                     LAARRLETAQRNRGRVGDYLAAQLELHKYPEEGFDQVLAATDLLPNFVRRWEAYLAEA
                     QKRSDPIFVPWHAFAEIPP"
     gene            complement(1570..1812)
                     /locus_tag="VFV87_08590"
     CDS             complement(1570..1812)
                     /locus_tag="VFV87_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103853.1"
                     /translation="MQEKLINLDNLLTAGAGAKTVPVTETRCGALSPAECDELKQLAI
                     LFAEVERELGESDDPDLRFAKAFAQQCALGDLGLID"
     gene            complement(1930..2868)
                     /locus_tag="VFV87_08595"
     CDS             complement(1930..2868)
                     /locus_tag="VFV87_08595"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5103854.1"
                     /translation="MYLLGTLRSYRSSRRIIMSHWKLAALLAICAGCTPQPQNVANKP
                     APSAMAPSAIAPSEPAGSKSETGNALKPLASGRFVDLSHPFDETTIYWPTEAGFVLER
                     GNNGVTPKGYYYAANRFRAAEHGGTHIDAPVHFSEGGHTVDQIELSRLIGEAVVIDVS
                     AKCEQDRDYLIGVADLRAWEEEHHRQLVDVIVLLRTGWAKRWPDRKSYLGTEARGPDA
                     VAELHFPGLAPEAARWLVENRAPKAVGIDTASIDYGQSTHFQTHVTLCEHNVPGFENV
                     ANLDELPAEGVLVMALPMKIAGGSGGPLRIVACVAP"
     gene            complement(2864..2937)
                     /locus_tag="VFV87_08600"
     tRNA            complement(2864..2937)
                     /locus_tag="VFV87_08600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2901..2903),aa:Arg,seq:tcg)
     gene            complement(3018..>4218)
                     /locus_tag="VFV87_08605"
     CDS             complement(3018..>4218)
                     /locus_tag="VFV87_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5103855.1"
                     /translation="PLAGSPSNPFPLLVAQEMPLVLESDATNDSLEAAQQVSVPAGIN
                     GRIEREGDVDYFAFEAKKGDAFSIEIVARRLQSSLDSHLRIVNDKGQQQQLSDDLKLG
                     KRSSADSWIENWTAPADGTYVIEVRDALLRGGPQFPYFLQITRSQPCFELYLDTDKTL
                     LGPGGNGAIFVRVERKNGFTGEVQLDIDGLPHGVVAHRGRILDGKGQDGCIVLEADVD
                     APPNVVNAVIRGTATHELPDGTRLELSAIATPYQETYQPGGGRGHWPVASHAIAVTEY
                     ADLRGISLSTYDVKLKPGESQKIEVTIDRSPEFTANVTLDMMMRHLNSNFANTLPPGV
                     TLDDKAAKSLLTGKETQGFLTITAAKDAAPVEKQQCVVLGHVALNFVMKWTYASRPII
                     VSVAKPE"
BASE COUNT          721 a         1326 c         1388 g          783 t
ORIGIN      
        1 ggcggaatct cggcaaaggc gtgccacggc acgaagatcg gatcgctgcg cttctgggcc
       61 tccgccaggt acgcttccca gcggcgaacg aagttcggca acagatcagt cgcagccagc
      121 acctggtcga agccttcttc cgggtatttg tgcagctcaa gctgcgccgc cagataatcg
      181 ccgacgcgcc cccggttgcg ctgggcggtc tccagacgcc gcgcggcgag cccctcggcc
      241 agcgcggcct tgcgtttttt aagctccgcc acgtaggtct gatgggccgg gtcttgcagc
      301 gccgccgccg tgagcggctc gatccgctcg atgcagcttt gaaaaacgcc gtacaacgag
      361 tagtagtcgc gagtcgggat cggatcgtac ttgtgatcgt ggcagcgggc acagccgacg
      421 gtcagcccca tcgtgccgcg ggtcacgacg tcgatgcggt cgtcgataat gtcgcgctgc
      481 accccgagga agcgtcggcc gagcgtgaga aaacccattg cggccagcga ctgcggatcg
      541 tccggcgcga cctgatcggc cgcgatctgg agcagcagga atcggtcgta gggcagatcg
      601 tcgttgagcg cctgcacaac ccagtcgcga tagacccagg cgtgcagcca gaagcgctgc
      661 tcgcgggcat agacgtagcc tttcgtatcc gaatagcggg ccacgtcgag ccaatgccgt
      721 ccccactgct cgccgtactg cggcgcggcg agcaatcgct cgaccaggtt ttcataagcc
      781 ttggggtctt ggtcgtcctc gaaggccgcc acttcgtccg gcgttggcgg caagccggtt
      841 aagtcatacg acgcgcggcg gatcagcgtc cgccggtcgg ccgacgggga tgaggacagt
      901 ttgtgctcgg ccaatttcgc acggatgaag gcgtcgatcg ggttgggcga gtcattgtcg
      961 gaaacggaag gaacggatgg atcccgtacc ggctgaaagg cccagtgcgt gcgggcggcg
     1021 tcggcagttt tagccgctgt accggaatcc ttcggccaag gcgcgccgag cttgacccaa
     1081 tgcgtaacgg cagcaagctg ggcctcggtc agctttccct tgggcggcat ctggatgtcg
     1141 ccctcgtggc gaatagccgc gatcagcagg ctcttggccg gatcgccggc cacgatcgcc
     1201 gggccgctca cgccgccttg cagcacgccc tctcgcgaat cgagccgcag gtccgattcc
     1261 tgcttcgtcg cgccgtggca cttctggcaa tgatcggcca ggatcggccg cacttcgcgc
     1321 tcgaaatact ccaccgccag cgggtcttcg gcctgacttt ggctcgctgc ggccacgaac
     1381 agcatcgcaa caaagctgcg caccatgcag tggatggaag gagccatcat ggaggcgggc
     1441 cgcggcgtcc ggcgggatgc gaacctgccg cggattcagg acgtcggccg atttccctaa
     1501 ggctaaacgg cccaagtgct ttcggcaaga gtgtgacaag ccgcgaggcc cgcggctgga
     1561 caaatcgtct cagtcgatca gccccaggtc gcccagggca cactgctggg cgaaggcttt
     1621 ggcaaatcgc aggtcgggat cgtccgactc gcccagctcg cgctcgacct cggcgaacag
     1681 aatagcgagt tgctttagct cgtcgcactc cgcgggcgag agggcgccgc agcgcgtctc
     1741 agtcaccgga acggtcttcg cgccggcgcc cgccgtgagc agattatcca aattgatgag
     1801 cttctcttgc atggcgcatt aagcctactg cgccgttcgg gattggggcg gcatgcgggg
     1861 cgggaaatct gggagaatcg ttccagtcgt gcctggcggc gctgatttgc cgggcagcgt
     1921 cggtacggtt catggcgcaa cgcaggccac gatgcgcagc gggccgccgc tgccgccggc
     1981 aatcttcatc ggcagggcca tgaccagaac gccttccgcc ggcagttcgt ccaggttggc
     2041 gacgttctcg aaccccggca cgttgtgctc acacagcgtg acgtgcgtct ggaagtgcgt
     2101 cgattgccca tagtcgatgc tcgctgtatc gatgcccaca gctttgggcg cgcggttctc
     2161 gaccagccag cgcgccgctt cgggcgccag gcccggaaaa tgcaactccg caacggcgtc
     2221 gggaccgcgg gcctcggtgc ccaggtacga cttgcgatcg ggccagcgct tggcccagcc
     2281 cgtgcgcagc agcacgatga cgtccacgag ctgccgatgg tgctcttcct cccacgcgcg
     2341 cagatcggca acgccgatca ggtagtcgcg atcctgctcg cacttggcag acacgtcgat
     2401 caccaccgcc tcgccgatca atcgcgacaa ctcgatttga tcgacggtat gccccccttc
     2461 agagaagtgg accggcgcgt cgatgtgcgt cccgccgtgc tcggctgcgc ggaagcggtt
     2521 tgccgcgtag tagtagccct tgggcgtcac gccgttgttg ccgcgctcca ggacgaaacc
     2581 tgcttcagtg ggccagtaga tcgtggtttc gtcgaagggg tgcgatagat cgacgaaacg
     2641 gccgctggcg aggggtttca gtgcattccc cgtttcggat ttggagcccg ccggctccga
     2701 tggggcgatt gccgatgggg ccattgcgga aggggccggc ttattcgcca cgttttgtgg
     2761 ctgcggcgtg cagccggcgc aaatcgccag cagggctgcg agcttccagt ggctcatgat
     2821 gatgcggcgc gatgagcggt acgatcgcaa cgttccgagc aagtacaccc agcaggactc
     2881 gaacctgcaa ccttcggttc cgaagaccga tgctctatcc aattgagcta tgggtgcgcg
     2941 gtgagcgaca cgctgcaaga aatcgtgccc tggcaccagg gtagcacaac ggcccagagc
     3001 cgcctattgc cggccggcta ctcgggcttg gcgacgctaa cgatgatggg ccggctggcg
     3061 taggtccact tcatcacaaa gttcaaggcg acgtgaccga ggacgacgca ttgctgcttc
     3121 tccacgggag cagcgtcctt ggcggccgtg attgtgagga agccctgcgt ctccttgccc
     3181 gtgagcagcg acttggcggc tttgtcgtcg agcgtcacgc cgggcggcag cgtgttggca
     3241 aagttggaat tgaggtgccg catcatcatg tcgagcgtca cgttggcggt gaattcgggg
     3301 ctgcggtcga tggtgacttc aatcttctgc gattcgcccg gcttgagctt cacgtcgtat
     3361 gtgctgagcg aaatgccgcg gaggtcggcg tattccgtca ccgcgatcgc gtgcgatgcg
     3421 accggccaat ggccgcggcc gccgccgggc tgataggtct cctggtaggg cgtggcgatc
     3481 gcggacaatt cgagcctcgt cccgtccggc agctcgtgcg tcgccgtgcc gcggatgacc
     3541 gcattcacga cattcggcgg ggcatcgacg tcggcttcca ggacgatgca gccgtcttgg
     3601 cctttgccgt ccagaatccg cccgcggtgg gcaacgacac cgtgcggcag gccgtcgatg
     3661 tcaagctgca cttcgcccgt gaatccgttc ttccgctcga cacgcacaaa gatcgccccg
     3721 tttccgccgg gtccgagcag cgtcttgtcc gtgtcgagat acagctcgaa acagggctgg
     3781 ctgcgcgtga tctgtaggaa atacgggaat tgcggcccgc cgcggagcaa ggcgtcgcga
     3841 acttcaataa catacgtgcc gtccgccggc gcggtccaat tctcgatcca cgaatcggcg
     3901 ctcgatcgct tgcccagctt cagatcgtcg ctcagttgct gttgctggcc cttgtcgttc
     3961 acgatgcgga ggtgcgaatc gagcgaggat tgcagccgcc gcgccacgat ctcaatggaa
     4021 aacgcgtccc ctttttttgc ctcgaaggcg aagtaatcga catccccttc gcgttcaatc
     4081 cggccgttga tgccagcggg gacgctgacc tgctgagccg cttccaggga gtcgtttgtg
     4141 gcatcggact cgagcaccag cggcatttct tgagcgacga gcagcgggaa cgggttggac
     4201 ggcgaaccgg caagaggc
//
LOCUS       DASUGB010000445         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_189824, whole genome shotgun sequence.
ACCESSION   DASUGB010000445 DASUGB010000000
VERSION     DASUGB010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189824"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFV87_08610"
     CDS             <1..819
                     /locus_tag="VFV87_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103856.1"
                     /translation="QASIDGQAVPVTPREPNVIRIDLPAPVRGHECVVELWYSMPTAS
                     NSASLLVDQLRPAILEEATPPRRTYWEVALPTSDYLLTTPSNLVKEMAWTTQNWQRGP
                     RPLLDQRQLETWIGSSRQDELPDGANTYLFGTLAQAPTLDVVVADRRLILAAASGLAL
                     AAGLLLIYVPWLRGATPLLIAAVVLAALALAYPDAALLLGRAALLGLGACASVWLWRW
                     LAQGRPAWTIATHSTATAPALGPSPSTTLPRGEKPAPITTATAPAGLAAGDSRA"
     gene            816..>4589
                     /locus_tag="VFV87_08615"
     CDS             816..>4589
                     /locus_tag="VFV87_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103857.1"
                     /translation="MNSLRVWTIAWLSALGLLGGHGAPAQEPLRSRAEDQLRYRRIYV
                     PEENLDREIRGLLPLKREEFERRIAQASRAASSPGGTLAARIDRASYRARLEGDQSLQ
                     GLARLEIACEDEGQTLLPLGECNLPLLGATWRGGPVRPAIVGHDQSGALGLLVDHRGT
                     VDADWSLRGVSTKDGIAFHLQLPPAAVSSLTLDLPADRSLSAEGGIVSPAAEGQSQPP
                     EEGRRAWLIEIGGTGRVKLLVESARAAEEPARRITVRETTTYSVLPASLDVEASWEVD
                     FQPHKPDTLAIRVPRPLVLTSIRSADRPLDWRVTEENDEASVATVSLAEPQATSNITL
                     QVQATGAWSAETPAALPRMTIADADFQEGRVTIAAPTWMRLQTTPIRGCWQTAVVPAT
                     GQRRIEQMEFRLLTAEAAIEIAADSSLPMLREWSGTQIMIDAAQINAVMIAELSSISR
                     ERFFAEAAISRRWIVDSIEVQPPEALSDRMLIAATPNQQLLRLNLQHPLQEAEPLRVL
                     IRAHQRRPADNQVLSGPLFRLAAFAGVSQPERIVAVRTLEPGVRLLWQGDQRVKRLDA
                     ARLTQDQMKRFEEAPSGVVFLDDDAMADMSAWLVTAAPVFQVQTALTAVVSERELEQQ
                     LAVRCTPQSSPIGRLVVRLSPKPTAPIAWSLPDEDSRELEVRPLASPDLAGDVAADDG
                     WYELVMRRPRAAAFEIQGRWSAPRTKRFALSLASVPEASVQAGMVEVRSRDGTQVFVE
                     ATGLQSLPIPTDTAERFTTLRGRYAWQPGRRASLEVLSSGGQVPAAAWIESLQVDTRF
                     SIDGGGQHEVTLAVSNQGDAQLRFRLPPTAIDVRQLTPLGGTLAPLARNSRGELQVAL
                     PPLERRATIRLSYRSDAAPLGWLSEGQFTAPLPQFNVPVLARRWHLAVAPGLEASSQA
                     SAQRSVDRWLRRLSPLVTPRRQPSGLLGTFDSPVAATLDAGETNAGGVSEANLETAGW
                     HRYDLELPAGQTASIRVYRPDVVAGLSIAALLLAAAIAVRFGTGRRSWLLPVVGIFLL
                     ASCFGAPQWFPVFTGLALGTLAGAVGLLMAASQPRTVPERATASTRVRSSVAALALVV
                     AIGNVLAISRAADESPPASNVKSAAAADHRVVIATTEQQQPTGDYVFVSPAFYERLHR
                     LTAAGSAALPDWLLVSARYQLLSAPKLSPAPSVDEIALHVAVETFRAGATLELPLRRD
                     QILLLEGRAKLDDEAVTVSWRDDGRAVIIPVDTAGQHQLELAFGAAVAKAEDHL"
BASE COUNT          723 a         1480 c         1565 g          821 t
ORIGIN      
        1 caggcctcga tcgacggaca ggccgtgccg gtgacaccgc gcgagcccaa tgtcattcgg
       61 atcgacttgc ctgccccagt gcgcggccat gaatgtgtgg tcgagctttg gtattcgatg
      121 ccgacagcat cgaacagcgc gagcctgctc gtcgatcagt tgcggccggc cattctcgag
      181 gaggcgactc cgccgcggcg cacgtactgg gaagtggcgc tgcccacgag cgactatttg
      241 ctgacgacgc cgtcgaacct ggtgaaggag atggcctgga caacgcagaa ttggcagcgc
      301 ggtccgcgcc ccctcctgga ccagcgccaa ttggaaactt ggatcggcag cagccggcag
      361 gacgagctgc cagacggcgc gaacacgtat ttattcggca cgctggcaca agcgccgacg
      421 ttggacgttg tcgtggccga ccggcgattg attctggcgg cggcttcggg gttggcgctc
      481 gcggcggggc tcttgctgat ctatgtgccg tggctgcgag gggcgacgcc gttgctgatc
      541 gccgcggtgg tgctggccgc gctggcacta gcgtatccgg atgccgcgct gttgctcggg
      601 cgggcggctc tgctcggcct cggcgcgtgt gcctcggtct ggctgtggcg ctggctggcg
      661 cagggccggc cggcgtggac cattgccacg catagcacgg ccaccgcacc ggctctgggg
      721 ccttcgcctt ccaccacgtt gccgcgcggt gagaagcccg cgccgatcac gaccgccacg
      781 gccccggccg ggctcgctgc cggagactcg cgggcatgaa ttccttgcgc gtctggacaa
      841 tcgcctggct gtcggcactt ggtctgctgg gcgggcacgg ggcgccggcg caagagccgc
      901 tgcgcagccg tgctgaagac cagctgcgct atcgccgcat ctatgttcca gaggaaaatc
      961 tcgatcgcga aattcgcggg ctgttgccgc tcaagcgaga ggaatttgag cggcgaattg
     1021 cccaggccag ccgcgccgcc agctcgccag gcgggacgct ggcggccagg atcgaccggg
     1081 cgtcgtatcg agcgcggttg gagggcgacc aaagcctcca gggactggca aggctggaaa
     1141 tcgcatgcga ggacgaaggg caaacgcttc tgccgctggg tgagtgcaat ctacccttgc
     1201 ttggtgcaac ctggcgggga gggcctgtcc ggccggcaat cgtgggccac gaccagagcg
     1261 gcgcgctggg cctcctggtc gatcaccgtg ggactgtgga tgccgattgg agcttgcgcg
     1321 gcgtcagcac caaggatggc attgcgttcc acttgcaatt gccgcccgct gcggtttcca
     1381 gtctgacgct cgatttgccg gcagatcgaa gcttgtcggc ggaggggggc attgtctcgc
     1441 ccgccgctga agggcaatcc cagccgccag aagaaggccg ccgagcatgg ctgatcgaga
     1501 tcggcggcac cgggcgcgtg aagctgctcg tggaatcggc gcgtgcggct gaagagccag
     1561 cgcggcgaat caccgttcgg gaaaccacca cgtattcggt tctgcccgcg tcgcttgatg
     1621 tggaagcatc ttgggaagtg gatttccagc cccacaagcc ggacacgctt gcgatccgcg
     1681 ttccacgacc gctggtgctg acttcgattc gatcggcgga caggccgctg gactggaggg
     1741 tgacggagga aaacgacgaa gcctccgtgg caacggtcag cctggccgaa ccgcaggcga
     1801 cgtcgaatat cacgctccag gttcaggcga ccggcgcctg gtcggcagaa acgccggcgg
     1861 cgctgccgcg aatgacgatt gccgacgccg actttcaaga gggtcgcgtc accatcgcgg
     1921 cccccacctg gatgcggctg cagaccacac cgatccgagg atgctggcag actgcggttg
     1981 tgccggctac cggccagcgc cggatcgaac agatggagtt tcggctgttg accgccgagg
     2041 ctgcaattga gatcgctgcc gattcgtcgc tgccgatgct gcgcgagtgg agcgggacgc
     2101 agatcatgat cgacgccgcc cagatcaacg ctgtcatgat cgccgagcta tcgtcgatca
     2161 gccgcgagcg attctttgcc gaagcggcaa tttcccggcg gtggatcgtc gattcgattg
     2221 aggtccagcc gcccgaggcg ctcagcgatc gcatgctgat cgcggccact cccaatcagc
     2281 agcttctccg cctgaacctg cagcatcctc ttcaggaagc ggagccgctc cgcgtgctga
     2341 tccgggcgca ccagcgccga ccggccgaca accaggtgct ctccggcccc ctgttccggc
     2401 tggcggcgtt tgccggcgtc tcccagccgg agcggatcgt ggccgtgcgc acgctcgagc
     2461 cgggagttcg cttgctatgg caaggcgatc agcgggtcaa gcgactcgat gctgcgcggc
     2521 tcacgcagga ccagatgaag cggttcgaag aggcgcccag cggcgtggta ttcctcgatg
     2581 atgacgccat ggccgacatg tcggcctggc tcgtgacggc tgcaccggtc tttcaagttc
     2641 agacggcctt gaccgcggtg gtcagcgagc gggaactgga gcaacaactg gccgttcgct
     2701 gcactcccca gtcgtcgccc atcggccggc tggtcgttcg cttgtcgccc aagccgaccg
     2761 ccccgattgc ctggtcgctg cccgatgagg atagccgcga attggaagtc cggccgcttg
     2821 cgtcgcccga tctggccggc gatgtcgctg ctgacgacgg ctggtacgag ctcgtgatgc
     2881 gccgcccgcg cgccgcggcg tttgaaattc aaggtcgctg gtcggcgcct cgaacaaaac
     2941 ggtttgccct cagcctggca tccgtacccg aggccagcgt gcaagcgggg atggtcgagg
     3001 ttcgttcccg cgatggaacg caggtcttcg ttgaagcaac gggtttgcag tcactgccga
     3061 ttccgaccga caccgccgag cgattcacca cgctccgtgg tcgatatgcc tggcagccgg
     3121 ggcgccgggc cagcctggag gtcttgtctt ccggcggcca ggttccggcc gcggcctgga
     3181 tcgaatcgct ccaggttgat actcggttct cgatcgacgg aggggggcag cacgaggtca
     3241 cgctggcggt cagcaaccag ggagacgcgc aacttcgctt ccgcttgccg ccaaccgcga
     3301 ttgacgtccg gcagctaacc ccccttggcg gcacactggc gccgcttgcg cggaattcgc
     3361 ggggtgaatt gcaggtggcg ctcccaccgc tggagcggcg agccacgatt cgcttgagct
     3421 accgatccga tgcggccccg ctcggctggt tgtcggaagg acagttcacc gcgccgctgc
     3481 cgcagttcaa tgtgccggta ctcgcgcggc gttggcattt agccgttgcg ccgggcctgg
     3541 aagcgagcag tcaggccagc gcgcagcggt cggtcgatcg ttggctgcgg cgactgagtc
     3601 cgctcgtgac tccccgccgg caaccgagcg gcttgctcgg cacgttcgat tctcctgtgg
     3661 cggcgaccct tgatgccggc gaaacgaatg cgggcggtgt ttccgaagcc aacctagaga
     3721 ctgccggctg gcatcgctac gatctcgagc tgcctgccgg tcagactgcc tccattcgcg
     3781 tctatcggcc cgacgtcgtt gcgggactat cgattgccgc cttgctgctg gcagccgcca
     3841 tcgcggtgcg ttttggcact ggccggcgga gttggctgct cccggtcgtc ggaatattcc
     3901 tgctggcatc gtgttttgga gcgccgcaat ggttcccagt cttcacaggg ttggcacttg
     3961 gcaccctggc cggggctgtc ggtttgctga tggcggcgag ccagcctcgc accgtccctg
     4021 aacgagcaac cgcctcgacg cgggttcgca gttccgtcgc agcgctggcg ctggtcgtgg
     4081 caatcggcaa cgtgctggca attagccgcg ccgcagacga atcgccgccg gcgagcaatg
     4141 tgaaatcggc cgcggcagcc gatcatcgcg tggtgattgc caccactgag caacaacagc
     4201 caaccggcga ctatgtattc gtttctccgg cattctatga gcggctacac cggctgacgg
     4261 cggcgggatc ggccgctctg cctgattggt tgctcgtttc agcccgctat caacttcttt
     4321 ccgcgccgaa gctgagccct gcgcccagcg tcgacgaaat tgccctgcac gtcgcggtcg
     4381 agacattccg tgcgggggcg accctcgaac tgccgctgcg gcgcgatcag attctcctgc
     4441 tcgaagggcg cgcgaagctg gacgacgagg ccgtcaccgt gagctggcgc gatgacggcc
     4501 gggcggtgat cattccggtc gatacggcgg gccagcatca gctcgagctc gcctttgggg
     4561 ccgccgtcgc gaaggccgag gaccacctg
//
LOCUS       DASUGB010000446         3984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1718691, whole genome shotgun sequence.
ACCESSION   DASUGB010000446 DASUGB010000000
VERSION     DASUGB010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718691"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV87_08620"
     CDS             <1..189
                     /locus_tag="VFV87_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103858.1"
                     /translation="GDDSTVSMINPQGLVLEYADHVEPLGRRYSFPHKEMAFPAQRGE
                     GARNATIGPTFRFDEAEK"
     gene            complement(305..436)
                     /locus_tag="VFV87_08625"
     CDS             complement(305..436)
                     /locus_tag="VFV87_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103859.1"
                     /translation="MEHACLVVHTVTFARWWRLKGTRYEVADESLSSFVFPDLLGTP"
     gene            986..1216
                     /locus_tag="VFV87_08630"
     CDS             986..1216
                     /locus_tag="VFV87_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103860.1"
                     /translation="MTTETISIAVDAEAARALAQASTEDRRKLELLLSLRLKELVGGP
                     SRPLSAIMDQMSAEAQARGLTPEALESLLQVE"
     gene            1389..>3984
                     /locus_tag="VFV87_08635"
     CDS             1389..>3984
                     /locus_tag="VFV87_08635"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5103861.1"
                     /translation="MRKIRIFVSSPGDVGEERSIAHRVIERLKFRYVGRANLEVLLWE
                     HLPLVATQSFQEQIPDAGQFDIVVMVIWSRMGSRLPAAIKRSDDSPYDSGTEYEFEQA
                     LEAHRRRGLPNLIVYRKTARVMVDLSAQEAELLELTRQKKSVESFIDRWFKSSDGSFQ
                     AAFHPFDSSAQFEDLLERHLRALVDAQIGSRPTGSVVVAGDTPLWDQGSPFRGLERFD
                     FEHGMIYFGRTAAVNDVLSALRQQAAAGCPLVLVFGKSGVGKSSFVRAGVLPLLCDPG
                     VIESIRHWRRAIFEPSDSTGDSLDGLAAALFTQTALPELKEATTEEELARLLRTSPEA
                     AIPLIRSALNGVAKNGSPHAPREDSGSRSETPADGITRSVMPTMPARLALAIDPLEEI
                     YSRPGASHEDRERLVAALDALARSGLVWVIGTMRSDFYERCGELPGLLALKAGSGQYH
                     LAPPTTAEIGRMIRLPAQLAGLSFEEDENHGTLDEVLKEAAAEDPAALPLLQFALDEI
                     YKRSKAAETGLLTFAAYNELGGIHGAIRTRAEETLAEVEQQLGERLPKTFANVFSLLV
                     GVNQVTEHSVVRRYAYLDRISADPDRKILVDALIAARLFMTDIDDENRPVVTLAHEAL
                     LHHWPRLSNWIEENRDFLRVRSRVASAAARWREEKREPAYLLSEGKPLVEAEHLLDRH
                     RGELAPETIEYIEASSAAVRAEHLRARQRSRRIMTAISTALVAALIFGVFSFFQYREA
                     SRQREIALENEKYAQGQEKIAKQKATEASEQRESAAKRARAAQELIEFLLADLRDSLA
                     PDNARDAGLIAKISKQVVDHYHSIDSASDTPEVKVEHANKLLGVANIFRKMARFDEAA
                     NLAQMSLDL"
BASE COUNT          750 a         1327 c         1204 g          703 t
ORIGIN      
        1 ggcgacgact cgaccgtcag catgatcaat ccgcaggggc tggtgctgga gtatgccgac
       61 cacgtggagc ccctcggccg ccgctactcg ttcccgcaca aggaaatggc gttccccgcc
      121 cagcgcggcg aaggcgcccg caacgccaca atcggcccca ccttccgctt cgacgaagcg
      181 gagaagtagc tatagcatcg ttttggagct gccgatgcgg tacagacttc ggacgcttct
      241 gatagttctt gccttgggtc cacccatgct ttggggcggt tactactgct ggggcagtta
      301 tacctcatgg cgtgccgagc agatccggga atacgaaact cgacagcgac tcatccgcga
      361 cttcgtaccg cgtccccttg agccgccacc accgcgcgaa ggtgacggtg tgtaccacta
      421 ggcaggcatg ttccatttca cgattcgcga catcctctgg ttgacggtcg taggcgcgat
      481 tgtcattgcg tggggattgg atcgtgctcg cttcaactcg cagctacgag aacggcaggc
      541 cacgattgac gctcgcgatc ccaagagcct tcgggtgatt agcctgagag cactccatga
      601 cagcttggcg cgcccgaatc ctgcagaatc ccctccgcca ttccaattcg agatccccct
      661 cgattagcaa taatgcagca gaaccgcaac gcttgcgtgg cagttcagcg gcgaaggtca
      721 gtcttcacgt ggaaatgaca ggaccatcaa ggacaggaaa acgggaatcg ccagtatttt
      781 cctgtcctca atctccctgt cttttctgga cctgcgggcc atggaagcct ggggccggct
      841 cgacagccaa ctccggccag tgaccaatcg ccccatcgac cgtttgagtg caccgccggc
      901 aaaggccccg tgagttgccg ccaacggagc ccaagcgaca agcggaagcc atgcggcgtg
      961 gtataatcca aggttggacg attccatgac taccgaaaca atctcgattg cggtcgatgc
     1021 cgaggcagcg agggccctgg ctcaagcttc gacggaagac cggcgcaagc tggagttgct
     1081 cttgagcctg cggctcaagg agctggtcgg tggtccgtca cggccactct cggcaatcat
     1141 ggaccagatg agtgccgaag cccaggcccg cggcctgacg cccgaagcgc tggagtcgtt
     1201 gctccaagtt gaatgagcgc aagcgcgggg ctccctcgct tacgcttcgg gttactgtgc
     1261 ccgatccgcg ttcttccgcg taaatctgcg gcgaaaaaac cagcttcttg atccggcttc
     1321 cccggccatc taagctgacg attcgtcctg cccctcgacc atcgccgccc gatcctcccg
     1381 cccaggctat gcgcaagata cgcatttttg tctcttcccc gggcgatgtc ggcgaggagc
     1441 ggtcgattgc ccaccgcgtg atcgagcggc tgaagttccg ctatgtgggg cgggcgaacc
     1501 tggaggtact gctctgggag catctgccgc tggtggccac gcagtcgttt caggagcaga
     1561 ttcccgacgc cggccagttc gatatcgttg tgatggtcat ctggtcgcgg atgggctcgc
     1621 ggctgccggc ggcgatcaag cggagtgacg acagcccgta cgattcgggg accgaatacg
     1681 aattcgagca ggccctggaa gcccaccgcc gccgcggcct gccgaacctg atcgtctatc
     1741 gcaaaacggc ccgcgtgatg gtagatcttt cggcccagga ggcggaactg ctggagctga
     1801 cgcgccagaa aaagtcggtt gaatcgttca tcgaccgctg gtttaagtcc tccgacggct
     1861 cgttccaggc ggcctttcat ccgttcgaca gctcggccca gttcgaggac ctcctcgaac
     1921 gtcatctgcg cgcactggtt gatgcccaaa tcggcagccg gccgactggc tccgtcgttg
     1981 tcgcggggga tacgccgctc tgggatcagg gctcgccgtt ccgcggcctg gagcggttcg
     2041 atttcgagca cggcatgatc tatttcggcc gcaccgccgc ggtcaacgac gtgctgagcg
     2101 ccctccgcca gcaagcggcg gccggctgcc cgctggtgct ggtcttcggc aaaagcggcg
     2161 tgggtaaatc gtcgttcgtg cgggccggcg tgctgccgct gttgtgcgat ccgggcgtga
     2221 tcgaaagcat ccgccactgg cggcgggcca tcttcgagcc cagcgactca accggcgatt
     2281 cgctcgacgg cctggccgcc gcgttgttca cccagacggc gctgcccgag ttgaaagagg
     2341 cgaccaccga agaagagctc gccaggctcc ttcgcacctc ccccgaagcc gccatcccgc
     2401 tcatccgcag cgcgctgaat ggcgtcgcca agaatggtag tcctcacgct ccgcgtgagg
     2461 acagcggctc gcgcagcgag acgcccgcag atggcatcac gcggagcgtg atgcccacca
     2521 tgcccgcccg ccttgccctc gccatcgacc cgctcgaaga aatctatagc cgccccggtg
     2581 cttcgcacga agatcgcgag cggttggtcg ccgccttgga cgccctggcc cgaagcggtc
     2641 tcgtctgggt catcggcacg atgcggagcg acttttacga acgctgcggc gaattgcccg
     2701 gcctcctcgc cctcaaagcc ggcagcgggc aatatcacct ggccccgccg acgaccgccg
     2761 agatcggccg gatgatccgc ctgccggcgc agcttgcggg tctctcgttc gaggaagacg
     2821 agaatcacgg aactctcgac gaagtcctca aggaagccgc cgcggaagat cccgccgccc
     2881 tgccgctgtt gcaattcgcc ctcgacgaaa tctacaagcg gagcaaagcg gccgaaacgg
     2941 gattgctgac tttcgccgcg tacaacgagc tgggcggcat tcacggcgcg atccgcacgc
     3001 gggccgaaga aacgctggct gaagtcgagc agcaactcgg cgagcggctg cccaagacat
     3061 tcgccaacgt cttcagcctg ctcgtcggcg tcaaccaggt gaccgagcac agcgtcgtcc
     3121 ggcgctatgc gtatctcgat cgcatctcgg ccgatcccga tcgtaagatt ctggtcgatg
     3181 ccctgattgc ggcccggctg ttcatgaccg acatcgacga cgaaaaccgt cccgtcgtca
     3241 ccctcgccca tgaagccctg ctgcaccact ggccgcggct ctcgaattgg atcgaggaaa
     3301 accgcgattt tctccgcgtc cgcagccgcg tcgcctccgc ggccgcccgc tggcgggaag
     3361 aaaaacgcga gccggcctac ctcttgagcg aaggcaagcc gctggtcgaa gccgagcacc
     3421 tgctggaccg gcaccgcggc gagctggccc cggaaaccat cgagtatatc gaggcttcca
     3481 gcgccgcggt gcgggccgag caccttcgcg cccgccagcg gagccggcgg atcatgacgg
     3541 cgatcagcac ggccctcgtc gccgccctga tcttcggcgt cttcagcttc tttcaatacc
     3601 gcgaggccag ccgtcagcgc gagatcgctt tggagaacga aaagtacgcc caaggccagg
     3661 aaaagatcgc caaacagaag gcaaccgaag ccagcgagca gcgcgaatcg gccgccaagc
     3721 gggctcgcgc ggcccaggag ctgatcgaat tcctcctggc cgacctccgc gacagcctcg
     3781 ccccagacaa tgcccgcgat gccggcctga ttgccaaaat ctccaaacag gtcgtcgatc
     3841 attaccactc gatcgattcg gccagcgata cgcccgaagt gaaggtcgag cacgccaaca
     3901 agctgctcgg cgtggcgaac atcttccgca agatggctcg tttcgatgag gccgccaatc
     3961 tggcccagat gtcgctcgat ctcc
//
LOCUS       DASUGB010000447         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1580383, whole genome shotgun sequence.
ACCESSION   DASUGB010000447 DASUGB010000000
VERSION     DASUGB010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580383"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..604
                     /locus_tag="VFV87_08640"
     CDS             338..604
                     /locus_tag="VFV87_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103862.1"
                     /translation="MATALARWDDEDGAPAVPSPLRYDVRGLPDTERRILECLGAALV
                     DEWSSLPTDIQRTIFQRATAGRVYDPAELRARIARFLHDHKDAR"
     gene            709..2022
                     /gene="nhaA"
                     /locus_tag="VFV87_08645"
     CDS             709..2022
                     /gene="nhaA"
                     /locus_tag="VFV87_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5103863.1"
                     /translation="MNDGLTRLPREPADRLTRPFMRFLRIEAMAGVVLLLSTFLALAL
                     SNTAWSAQFLAFWEMHAGFTLGGLEFSRSLKHWINDGLMTLFFFVIALELKRELVLGE
                     LRNPRMAALPVAAALGGMVVPAGLFLLLVRGGPGASGWGTVMSTDTAFVIGCLAILGS
                     RVPQSLRLFLLSLAIFDDVGAILVVAIGYGGALNWIAISLVGLGLAVVAGSARLGIRS
                     IPVYFAIGGGIWLALDASGVHATLAGVVLGLMTPARSWVSDRRLHAIFDRVIAYPPGE
                     HWSSDKVARRDLRQAGVAAREALSPIERLEFALHPWVAFTIMPLFALANAGVPIAAVS
                     FDQTLTTAIFFAFVVGKPVGVVLFSFLAVKLRAGILPNELPWSVLAAGSLLTGIGFTM
                     ALFIAELAFDASLLDSVKLGVMGASVISAAAGFLTLVWLTSSRRR"
     gene            complement(2083..2823)
                     /locus_tag="VFV87_08650"
     CDS             complement(2083..2823)
                     /locus_tag="VFV87_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5103864.1"
                     /translation="MSRAPRLRPIKPNTNRKAASEVPFDPAVFLSTVALGRDISVHSK
                     KEVIFAQGDDADAVFYIKKGKVKVAVVSKQGKEAVVAILGPDEFVGEGCLIGQPKRLA
                     TASAMTECETMRVAKTEIQRLLRDEPAFSKMFVSHILARNARIEEDLVDQLFNSTEKR
                     LARLLLLLANFGKEGQTEPILAKISQETLAEMIGTTRSRVSHFMNKFRTLGFIDYNGH
                     LKVHNSLVGVLLVDQPRTVSRPRGGISQ"
BASE COUNT          510 a          887 c          881 g          662 t
ORIGIN      
        1 acgcagttgc ccgtggtctc ggaggtttat tggtcgcatc cgcagtcgga gagtttgccg
       61 cccacgccgg catgccggct tggcagatcg gacttatcgt tttgcttgtg gcaatcggtc
      121 tccccctgtg tttaagcggg cgtcttggga attggtgaca atttcgtagg tttggaccag
      181 gtgctggaat tccggcggca gaaatatcgc aattcaattt ttgtacctga aatccacgcg
      241 atagccgctc cacgagatcg ggactaccgc atgctgtttg tgcgatggtg cgcgaatggc
      301 ggagaagaag tcgcggcgcg cgaaaacccg gaaacagatg gctaccgctc tcgcccggtg
      361 ggacgacgaa gacggggcac cggctgtgcc gtcgcccttg cgctacgatg tgcgcggctt
      421 gccggacacg gagcgccgta ttttggaatg tctgggcgcc gccctggtcg atgagtggag
      481 cagtctgccg accgatatcc agcggaccat ttttcagcgt gcgaccgcgg gccgtgttta
      541 tgatcccgca gagttgaggg ctcggatcgc tcgcttcctg cacgatcaca aggacgcgcg
      601 gtaggcgctg cagggaatgc aggcaccagt ctctcggctg ggcacccgac cagaaggcag
      661 gactcacagg caaattgcca tcgggagatg gaaacgaagg tttatggaat gaacgatggc
      721 ctcacgcggc ttcccaggga gccggcagac cggctcacga ggccgttcat gcgattcctg
      781 cgcatcgagg cgatggctgg cgtcgtcctg ttgctcagca cgttcctcgc gctggcgctc
      841 tccaatacag catggtccgc gcaattcctc gccttctggg aaatgcatgc ggggttcacg
      901 cttggcggac tcgagttctc tcgttcgctg aagcattgga tcaacgatgg gctgatgacg
      961 ctctttttct tcgtcatcgc cctcgagctg aaacgcgaac ttgtgctggg agagttgcgc
     1021 aatccgcgga tggcggcgct gcccgtcgcc gccgctctcg gcggaatggt ggtgccggcc
     1081 gggctgtttc tgctgctggt ccgtggcgga ccgggtgcga gcggctgggg caccgtcatg
     1141 tccaccgata ccgccttcgt catcggttgc ctcgctatcc tggggtctcg cgttccgcaa
     1201 agcctacggc tgttcctact ctctctcgcg atcttcgacg acgtcggcgc cattctggtc
     1261 gtcgcgatcg gctatggcgg ggccttgaac tggattgcga tcagcctggt cgggctcggg
     1321 cttgccgtcg tggccgggag cgcgcgcctg ggcatccgca gtattcccgt ctatttcgcg
     1381 atcggcggcg gcatctggct ggcgctcgac gcatcgggcg tccacgcgac gcttgcgggc
     1441 gtagtcctcg gattaatgac acccgcgcgt agctgggtca gcgacaggcg tctgcacgcg
     1501 atcttcgacc gtgtgatcgc ttatcctccc ggtgagcact ggagcagcga caaggtggcg
     1561 cgccgcgacc tccggcaagc cggcgttgcc gcccgagagg ccttgtcacc gatcgagcgg
     1621 ttggaattcg ctctccatcc ctgggtggcg ttcacgatca tgccgctctt tgccctcgcg
     1681 aacgcgggcg ttccaatagc ggcagtgagc ttcgaccaga ctcttaccac ggctatcttc
     1741 tttgccttcg tagtcggcaa gcccgtcggg gtcgtgctgt tcagcttcct ggccgtgaag
     1801 ctgcgcgcgg gaatccttcc gaacgagttg ccgtggagcg ttttagccgc cggaagccta
     1861 ctgaccggca tcggcttcac tatggcgctg ttcattgccg aactggcgtt cgatgcctcg
     1921 ctcctggact ctgtcaagct tggcgtcatg ggtgcctcgg tcatctcggc cgcggcggga
     1981 ttcctgacgt tggtctggtt gacgtcctct cgcaggcgat aacgcgccaa gccgccagcc
     2041 ggcttggcct tcaccctgcc cccgcaagaa gcggagccgc gctcactggg agataccacc
     2101 ccttggccgg ctcacagtgc gtggctgatc gaccagaagc acgcccacca aagaattatg
     2161 aactttaaga tgtccgttgt aatcaatgaa acccagtgtg cggaacttat tcatgaagtg
     2221 gctcacgcgc gaccgggtcg tgccgatcat ctcagcgagt gtctcctgac tgatcttcgc
     2281 gagaattggc tccgtttggc cttcctttcc aaaattggcc aacagcagca acaaccgcgc
     2341 aagccgcttc tcggttgagt tgaagagctg atcgaccagg tcttcttcaa tacgtgcgtt
     2401 ccttgccaga atatgcgaga caaacatttt cgaaaatgcc ggctcgtcgc gaaggagccg
     2461 ctggatttcc gttttagcca cccgcatcgt ctcgcattcc gtcattgctg atgccgtcgc
     2521 cagacgcttc ggctgtccga tcaggcatcc ttcgccaacg aattcatcag gtcccaagat
     2581 cgcgacaacg gcttctttcc cctgcttgga tacaacggcg actttgacct tgcccttctt
     2641 gatatagaaa accgcatccg catcgtcacc ctgagcgaaa atgacttctt tctttgaatg
     2701 cacggaaatg tcgcggccaa gggccaccgt tgacaaaaac accgcaggat caaacggcac
     2761 ttcgctcgct gctttgcggt tcgtatttgg tttgatgggc cttaatctcg gtgcacgaga
     2821 cactatggct tcccttctat tcagaaatgc gcgcttccga gcgtaagtga cctagatagt
     2881 tcgcgaagct ctcgataggc cgcgttagtt cacggtctcg tgaaccccac tcagcgcggc
//
LOCUS       DASUGB010000448         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_885455, whole genome shotgun sequence.
ACCESSION   DASUGB010000448 DASUGB010000000
VERSION     DASUGB010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885455"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1065)
                     /locus_tag="VFV87_08655"
     CDS             complement(322..1065)
                     /locus_tag="VFV87_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX5103865.1"
                     /translation="MTQPRQVDVYLLPSLVEPQELAGKLVVVVDILRATTTIVHALAA
                     GAVQVVPCLEIDEARRLAGEFAGPALLGGERSGGKIPGFDLGNSPQEYTPASVGGKTL
                     VFTTTNGTQALARCRAARRVLLGAFINLSAMCRELAGEDHVTVVCAGTNGQVTREDAL
                     FAGALADELTAAGKWTLNDQGQIVRDAWRSAAANLVADPLSRTLRSSKGGRNLIGIGH
                     ENDIALAAEIDKFDLVPELDLATWRIRPA"
     gene            1214..2047
                     /locus_tag="VFV87_08660"
     CDS             1214..2047
                     /locus_tag="VFV87_08660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5103866.1"
                     /translation="MSDRALVSKVEIEAHKLADLGGRFDGKVALITGASDRGIGGEIA
                     VRLAREGAAVAMISRREPGRLLKKLSRFPGGAVHTLGDVTKGDDIERAIEACVDEFGK
                     IDVLVNNAGVEAASPLEAASDDQWRALLEVNLSGAIAVTKAALPVMVQPGSAIINIAS
                     ALGIGGCAGFSVYSASKAGLIGFTQSLAWELAPQGIRVVALAPGLVHTPMVHKHVEKL
                     TPDVWRQIEACHPLGMGRPADVAAAVAFLASDEARWITGVTLPLGWAPHYPLPSGALM
                     A"
     gene            complement(2153..>2605)
                     /locus_tag="VFV87_08665"
     CDS             complement(2153..>2605)
                     /locus_tag="VFV87_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103867.1"
                     /translation="VAGCWLWLKCGPPECGPLECGPLVPSGQEAAIDPAGTNSPDAMA
                     QATSTRRALAIATAVITLVVLAFYLTRPQMDRNYGGGTCCLRWLVWLTPLWLLTMLPV
                     ADRLGRSWWGRSLGLALLAISVFSAHYAADNPWENPWIYNLGQWLGGV"
BASE COUNT          448 a          817 c          917 g          423 t
ORIGIN      
        1 cttgatgttc cgcttcagct cgacccccag cgggccatag tcccaaaagc cgttcaggcc
       61 gccgtagatt tcgctcgact gaaagagaaa tccgcgccgc cggcagaggg cgacgagttt
      121 gtccatgtcc atggggaggg ctgggggcaa ggggctaggg ggctagaggc tcgaggctag
      181 aggctagagg gtggtgggga ggggctcgct tgttctggct gacagcgcga ttctgcgagg
      241 ctctgccgcg caagtcacga cgccagttta ccggcagagg ttcagaaggg tctatggcag
      301 aggcgcgacc gctggtcgcg gctatgcagg gcggattcgc cacgttgcca ggtcgagctc
      361 tgggacgagg tcgaacttgt cgatctcggc cgccaaggcg atgtcgttct cgtgcccgat
      421 gccgatcagg ttgcggcccc ctttactgct gcggagcgtg cggctgagcg ggtcggccac
      481 caaattggcg gcggcggagc gccaggcgtc gcgtacgatc tgcccctggt cgttgagcgt
      541 ccacttgcct gctgcggtca gctcgtcggc cagcgcgccg gcaaacagcg cgtcctcgcg
      601 cgtcacctgg ccgttggtcc cggcacaaac gacggtcaca tgatcctcgc cggccagttc
      661 gcggcacatc gccgagagat tgatgaacgc cccgagcagt acgcggcgag cggcccggca
      721 gcgggccagg gcctgcgtgc cgttggtcgt cgtgaagacc agcgtcttcc cgcccaccga
      781 cgccggcgtg tattcctgcg gcgagttgcc caggtcgaag ccggggattt tgccgccgga
      841 tcgctcgccc cccagcaggg ccggccccgc aaactcgccc gccagccgac gtgcctcgtc
      901 gatttccagg caggggacaa cctggaccgc gccggccgcc aaagcgtgaa cgatcgtcgt
      961 cgtggcccgc aggatatcga cgacaacgac gagcttgccc gccaactcct gcggctcgac
     1021 caggctcggc aagagatata cgtcaacttg tcgcggctga gtcatccgat aggcgcggtt
     1081 tagtaccgag cggcggctcg aacacggcac tgccgtgcgt tgacggggct ggtcagcgag
     1141 gaaacaatca gtctatcgac caagcgggat tcgtcccaga cgattgccac cttgctgccg
     1201 caccacgacg aacatgagcg accgcgcgct ggtttctaag gttgagattg aagcccataa
     1261 gctggccgat ttgggcgggc gctttgatgg gaaggtcgcc ctgatcaccg gggcgagcga
     1321 ccggggcatc gggggcgaaa tcgccgtccg cctggcccgc gaaggggccg cggtggcgat
     1381 gatctcgcgc cgcgagccgg gccgcctgtt gaaaaagctg tcccgctttc ccggcggggc
     1441 ggtccacacg ctgggagacg tgacgaaggg ggacgacatc gagcgggcga tcgaagcgtg
     1501 cgtcgatgag ttcggcaaga tcgacgtgct ggtcaataac gccggagtgg aagcggccag
     1561 ccccctggaa gcggccagcg acgaccagtg gcgggccctg ctcgaggtga acttgagcgg
     1621 cgcgatcgcc gtgacgaagg cggccctccc ggtgatggtg cagcccggca gcgcgattat
     1681 caacatcgcc tcggcgctgg gaatcggcgg atgcgcgggc ttttcggtct atagcgcgag
     1741 caaggcaggg ctgatcggct tcacgcagtc gctggcgtgg gaactagcgc cgcagggcat
     1801 ccgcgtcgtg gccctcgcgc cggggctggt ccacacgccg atggtacaca agcacgttga
     1861 aaagctcact ccagatgtct ggcggcagat cgaggcctgc catccgctgg gaatggggcg
     1921 gccggccgac gtggcggctg cggtggcgtt tttggcctcg gatgaagccc gctggatcac
     1981 cggcgtgacg ctgccgctgg gctgggcgcc gcattacccg ctgccgagcg gagcgctgat
     2041 ggcttaggga gtgtggccgg tgcgagtgta tgagagtgcg agagtatgag agtgtgaggg
     2101 aggggtcctg ctctcacaac caacaccctc gcaccctcac accctcgcac cctcacacgc
     2161 cccccagcca ctggccgagg ttatagatcc atgggttctc ccacgggttg tcggcggcat
     2221 aatgggctga gaagacgctg atggccaaca gtgccagccc cagcgatcgg ccccaccagg
     2281 accggccaag gcggtccgcg acgggcaaca tcgtcagcag ccacagcggc gtgagccaga
     2341 cgagccaccg caggcagcag gtgccgccgc cgtagttgcg gtccatctgc gggcgggtga
     2401 gatagaacgc gagcaccacc agcgtgatga ccgcggtggc gatggccaag gcgcggcgag
     2461 ttgaagttgc ttgtgccatt gcgtctggcg aattggtgcc ggctgggtcg attgccgctt
     2521 cttgtccgct ggggaccagc ggaccacatt ccagcggacc acattccggc gggccacatt
     2581 tcagccacag ccagcagccg gcgac
//
LOCUS       DASUGB010000449         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1996638, whole genome shotgun sequence.
ACCESSION   DASUGB010000449 DASUGB010000000
VERSION     DASUGB010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996638"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VFV87_08670"
     CDS             <1..1380
                     /locus_tag="VFV87_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5103868.1"
                     /translation="GLLAAGAKDQARQVIDYLRGTQEADGHWPQNCWLDGTAYWNGVQ
                     LDECALPILLVDLAFRESALSEEELPSVWPMIEKAAGFIVRNGPVTGQDRWEEDGGYS
                     PFTLAAEIAALLAAADLADRLAAPRQAAYLRETADIWNSLIEHWTYAADTPLARQVGV
                     DGYYVRIASPETADAASPVGGFVPIKNRPPQASCQQASLMVSPDALALVRFGLRAADD
                     PRILDTVRVIDALLKVDLPAGPCWHRYNGDGYGEHEDGRPFDGTGIGRCWPLLTGERA
                     HYELAAGNKIGAKTLLAALEGFASDGHLIPEQVWDAPDIPERELFHGKPSGSAMPLAW
                     AHAEHVKLLRSLADGRVFDMPPQPRERYQVQGVQRRHAVWRFNNKCRHVPAGHALRVE
                     LSSAALVHWSVDGWLTAHDTPAEDSGFGIYFADLGTTGFKSGDAILFTIFWPAENRWE
                     GVDYQLAVE"
     gene            1769..>2161
                     /locus_tag="VFV87_08675"
     CDS             1769..>2161
                     /locus_tag="VFV87_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5103869.1"
                     /translation="MKPTRKLHDIGQSLWLDNFTRELLNSGTLRRYIDQLSVTGLTSN
                     PTIFGHAIKNSTSYDEDVARKSSLTKSAEELLFDLALTDLARAADLFQPIFARTDGVD
                     GWVSLEVSPLLAHDTKSTLRAARDLHRRS"
BASE COUNT          387 a          664 c          691 g          419 t
ORIGIN      
        1 gggctgcttg ccgcgggggc gaaagatcaa gcgcggcagg tcatcgacta tctgcgcgga
       61 actcaggagg cggacggtca ctggccgcaa aattgctggc tcgatggcac ggcctattgg
      121 aacggcgtgc agctcgacga atgcgccctg ccgatcctgc tcgtcgacct cgcttttcgc
      181 gagagtgcgc tgtcggagga ggagttgccg agcgtctggc cgatgatcga gaaggcggca
      241 ggattcatcg tccgaaacgg cccggtcacg ggtcaggacc ggtgggaaga ggacggcggc
      301 tattcgccat tcactcttgc agccgagatc gccgcgctcc tggctgccgc cgatttagcc
      361 gaccgtttgg ccgcgccgag gcaagctgcc tatctccgcg agaccgctga tatctggaac
      421 agcctgatcg agcactggac ttatgctgct gacacgccac tcgcccgcca agtcggggtc
      481 gatggctact acgtccgcat cgcctcgccc gaaacggccg acgccgcgtc accggtgggc
      541 ggcttcgtcc cgatcaagaa tcggccgccg caagcgagct gtcagcaagc ttccctgatg
      601 gtgagccccg atgcgctcgc gctcgtgcgt ttcgggctgc gggccgcgga cgatccgcgc
      661 attctcgaca ccgtgcgggt cattgacgcg ctcctgaagg tcgatttgcc cgcaggaccg
      721 tgctggcatc gctacaatgg cgacggctac ggcgagcacg aagacggccg gccgttcgac
      781 ggcaccggga ttggacgctg ctggcccctg ctgaccggcg aacgggcgca ctatgagctc
      841 gctgcgggta acaagatcgg ggccaagacg ttgcttgcgg cgctcgaagg cttcgcgagc
      901 gacggtcatc tcattcccga gcaggtttgg gatgcgccgg acattcccga gcgtgagctt
      961 ttccacggca agccgtccgg ttcggcgatg ccacttgcct gggcgcatgc cgagcatgtc
     1021 aagctcctgc gctcgctggc ggacggtcgc gtcttcgata tgccgccaca gccgcgagag
     1081 cgttatcaag tccaaggcgt gcagcgacgc catgctgtct ggcgcttcaa caacaaatgt
     1141 cgccacgtgc cggcgggaca tgctttgcgg gtcgagcttt cctcggcggc tctggtccat
     1201 tggagcgtcg acggttggct caccgcccat gacactcctg cggaggatag cggattcggc
     1261 atctacttcg ccgatctagg cacgacgggc tttaaatccg gcgatgccat cttgttcacc
     1321 atcttctggc ctgccgaaaa tcgctgggaa ggtgtggact atcagttagc ggtcgagtga
     1381 tcggctttcc gctacagtcg tctggtatgc ggatgtgcat gaccgacctg atggtcgtca
     1441 caggggtacc ggcttcaaag tcccgctgag cgttcggcga tattgggcct tgtggatggg
     1501 cacgggtcta tccatatcca taagggagaa gccgtagatg acgtcgtgcg ccgctaccat
     1561 ggcgacgcgc ggcgctgagg caattctggg aacggcgggt gacagcttgg tcctccgcgg
     1621 caggaagtct gtgcccatga attgtgaatc gtcgccgact ttcctccaac agacgtgacg
     1681 atcgacgcag cgggggcgcg catgattgtg gcgtctcgtg gttgcgtcac ggtcaccttt
     1741 ccgagggtga cgtcttgaag gtcacgccat gaaaccaacc aggaagctcc acgacattgg
     1801 ccagagtctc tggctcgaca acttcacccg cgaactcctg aacagcggca cccttagacg
     1861 ctatatcgac cagctctcgg tgaccggact cacctcgaac ccgacgatct tcggccatgc
     1921 gatcaagaac agcacgagct atgacgaaga cgttgcccgg aagtcgtccc tgacaaaatc
     1981 ggcagaggaa ctgttgttcg atctggcgct gacagacttg gcccgcgccg ctgatttgtt
     2041 ccagcccatc ttcgcccgga ccgatggcgt ggacggttgg gtgtcgctgg aggtttctcc
     2101 tctgctggcc catgacacaa agagcaccct gagggccgcc cgggatctcc ataggcgcag
     2161 c
//
LOCUS       DASUGB010000450         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1996850, whole genome shotgun sequence.
ACCESSION   DASUGB010000450 DASUGB010000000
VERSION     DASUGB010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996850"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VFV87_08680"
     CDS             <1..978
                     /locus_tag="VFV87_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103870.1"
                     /translation="DCGIDSDRAAAYKVGMLSTAEPEAMPRPANSLVTQWLTHLERRS
                     LPHFNQLVIWVLLATGAILGCWVFEIHEGPRQGTATFLFWLPHAILSSLWLNWLFRGL
                     LFAGIGLWLFQKGLPWSCWLVVVGFTGVWSLHVENTHNAAHIFHMANTLLVIQAIWIT
                     ADAPLIKRALADGTYWTTPLVPRWVSLASIAYIGLFHTAAGVSKLWFSGPEWASGTSL
                     QIWTYLWGRRASPTTQMILASRTFTQVLQAATLVVETAGVLAIIPRLRLWIGLGLLAF
                     YVGVLATFDYGFQFNALLTAMYLLPLEPWLTRRVENKLSKNEPKNTSRN"
     gene            1206..>2421
                     /gene="glgP"
                     /locus_tag="VFV87_08685"
     CDS             1206..>2421
                     /gene="glgP"
                     /locus_tag="VFV87_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5103871.1"
                     /translation="MTTIDPAIVPESLPLLVGEMSAQSLYDKCWSLARNLWWTWHPEV
                     INLFRDLDPIRWRQLDHNPIALLREFTPDRLAQRAAEMVLYSRINYAHRRLKEYMANK
                     QTWAAWNAGVLGGKPVAYFSAEFGLHESIPIYSGGLGVLSGDHIKSASGLGVNLIAIG
                     LFYDQGYFKQQLDDSGWQREEYIDTKVENVPMEPALSPDGKPITVRVDTRNGPLFAKV
                     WMMQVGRVKLFMLDCDVEGNSPQDRGLTSRLYGGDERTRVRQELVLGVGGVKALRALG
                     IVPGVYHLNEGHSAFGPLEVIRERMHDDGLRFDDALREVARQTVFTTHTPVPAGHDRF
                     NAALVEEHLGPLRDQLGISHEQLMGLGRVEPQNSQETFCMTVVGLKISRKANAVSQLH
                     GHISRRMWAALWP"
BASE COUNT          432 a          775 c          739 g          475 t
ORIGIN      
        1 gactgcggca tcgacagtga ccgagccgcc gcatacaaag tcggcatgct ctcgactgcg
       61 gaacccgaag caatgccgcg cccggccaat tcgctggtga cgcagtggct gacacacttg
      121 gagcgccgct cgctgccgca cttcaaccag ctcgtgatct gggtgctgct cgcaacgggg
      181 gcgatcctcg gctgctgggt ctttgagatt cacgaaggcc cacggcaggg aactgcgact
      241 ttcttgttct ggctgccgca tgccatcctc agcagtctgt ggctgaattg gctctttcgc
      301 gggctcctct tcgccggcat tgggctgtgg ctgtttcaaa agggactgcc ctggtcgtgc
      361 tggctggtcg tcgttggctt tacaggagtc tggtcgctgc acgttgaaaa cacacacaac
      421 gcggctcaca tctttcacat ggcgaatacg ctgctcgtga tccaggcgat ctggatcacc
      481 gcggatgctc cgttgatcaa gcgggcgctg gccgatggca cctactggac gacaccgctg
      541 gtgccgcggt gggtatcgct cgcgtcgatc gcctacatcg gcctcttcca cacggcggcg
      601 ggggtttcca agctctggtt cagcggcccc gagtgggcca gcggaacttc gctccagatc
      661 tggacctatc tctggggccg acgcgcttcg ccgaccacgc agatgatcct cgccagccgc
      721 acgttcacgc aggtcctgca agcagcgacg ctggtggtcg aaaccgccgg cgtcctggca
      781 atcatcccga ggttacggct ctggatcggc ctgggactgt tggctttcta cgtcggtgtc
      841 ctcgccacat tcgactacgg cttccagttt aatgccctgc tcaccgccat gtacctgctg
      901 ccgctcgaac cgtggctcac gcgccgcgtg gaaaacaagc tttcaaagaa cgagccaaaa
      961 aacacgtcgc gaaactgaca gcacgcaccc cttgccgaag atggcgcgtg gtcgaattcg
     1021 cttgaacctg acaagggcga gcaatctcac gttatgggcc aactagcggg tcttggggcg
     1081 gtttgtttca ccaactgctt gacacatcca gtttccagcc gatatcaagt gtctagactg
     1141 cctatcatcg tgcctttgag gcgcgctttt atattcgtac ttgcagggcg gcgaaggagg
     1201 tttgcatgac gaccatcgat cccgcaatcg ttcccgaatc cttgccgctg ctcgtgggcg
     1261 agatgtccgc tcagtcgctc tacgacaagt gctggagcct ggcccgcaac ttgtggtgga
     1321 cctggcaccc ggaagtcatc aacctgtttc gcgatctgga tccgatccgc tggcggcagc
     1381 tcgatcacaa cccgattgca ctcttgcgcg agttcacccc cgaccgcctg gcccagcggg
     1441 ccgccgaaat ggtcctctac agccggatca actatgccca ccgccggctg aaggagtaca
     1501 tggccaacaa gcagacctgg gcggcctgga acgcgggcgt tctggggggc aagccggtcg
     1561 cctacttctc ggccgaattc ggcttgcacg agtcgatccc gatttattcc ggcggtctcg
     1621 gcgttctctc gggcgaccac attaagagcg ccagtggcct gggtgtgaac ctcatcgcca
     1681 tcggcctctt ttacgaccag ggctatttca agcagcagct cgacgacagc ggctggcagc
     1741 gggaagagta tatcgacacc aaggtcgaaa acgtgccgat ggagcccgcc ctctcaccgg
     1801 acggcaagcc gatcaccgtt cgggtcgata cccgcaacgg cccgctcttc gccaaggtgt
     1861 ggatgatgca ggtcggccgc gtgaagctgt tcatgctcga ctgcgacgtg gaggggaaca
     1921 gcccgcagga ccgcggcctc accagccgtt tgtacggcgg cgacgagcgg acgcgcgtcc
     1981 gtcaggaatt ggtgctgggt gttggcggcg tcaaggcgct gcgggcgctg ggcattgtgc
     2041 cgggcgtcta tcatctcaat gaagggcaca gcgccttcgg cccgctggaa gtgatccgcg
     2101 agcggatgca cgacgacggc ctgcgcttcg acgacgccct gcgggaagtc gcccggcaga
     2161 cggtctttac cacgcacacg cccgttcccg ccggtcacga ccgcttcaat gccgccctgg
     2221 tcgaagagca tcttggaccg ctgcgcgacc agcttggcat cagccacgag cagctcatgg
     2281 gcctggggcg cgtcgagccg caaaacagcc aggaaacgtt ctgcatgacg gtcgtgggcc
     2341 tgaagatttc ccgcaaggcc aacgccgtga gccagcttca cggccatatc tcccgccgca
     2401 tgtgggcggc gctctggccg t
//
LOCUS       DASUGB010000451         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_51371,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000451 DASUGB010000000
VERSION     DASUGB010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51371"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFV87_08690"
     CDS             complement(<1..251)
                     /locus_tag="VFV87_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103872.1"
                     /translation="MKSPSPQGPASGSLVHPPLWGTLCGLASAVGYTCANAFLRSVDD
                     YDPFWVSAVKALPTIVAMSPVIAWMALRGQRVWPGANLLA"
     gene            281..823
                     /locus_tag="VFV87_08695"
     CDS             281..823
                     /locus_tag="VFV87_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX5103873.1"
                     /translation="MSLPSIARRVAHLLEQAERKVVFAESCTGGLVSATLARIAGISN
                     HLCGGVVVYRNATKQAYLQIPADVLEDPGPVSDVVAREMALRVLERTPEADIAASVTG
                     HLGPNAPRYLDGVVFTGIAVRSTNRRASQHVKVRRLRCRTADARPARQRWVTEQLLQW
                     LAAELEALAAAPSPTGRGWG"
     gene            859..1650
                     /locus_tag="VFV87_08700"
     CDS             859..1650
                     /locus_tag="VFV87_08700"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5103874.1"
                     /translation="MVFPLSDDNSDRTQFPVVTVSLIVINVLVFVLLQGAGMNEGFTM
                     AFSTVPKEIVTGTDLVTLDRQIEIETEEGPQTVTAPGLKKTPVSVYLTLLTSMFMHGS
                     IMHIAGNMWFLWIFGDNIEQDLGRIRYVLFYLLAGLLASLTHVLFNASGPSAEVPSLG
                     ASGAISGVMGAYLVLHPHRRVTVLLFRFVTQVPGYVAVGIWFAFQIVSGWLDRGQGGV
                     AYGAHIGGFIAGAALAFPFMLGRPRKENRGYVQKREYDKGIHWGR"
     gene            1775..>2262
                     /locus_tag="VFV87_08705"
     CDS             1775..>2262
                     /locus_tag="VFV87_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103875.1"
                     /translation="MSTRSTASWPFALAAAAALVLLSNTAWAGEVGVRIRFGLTDTEP
                     TDWSGTATANPGKVVLISGWRFEQDDEAKGTEGWSASTRGAAATGRTNAQKGKAKAKA
                     GAGAAAKAKAKAKAKAGAAATMADNGVLLTLADVTEASVVNVKTEEGDFQFKISEIPY
                     GKV"
BASE COUNT          430 a          713 c          715 g          404 t
ORIGIN      
        1 gcgagcagat ttgctcccgg ccatacccgc tgcccacgca gtgccatcca ggcgatgacc
       61 ggcgacatgg caacgattgt cggcagggct ttgactgccg agacccagaa agggtcgtag
      121 tcgtcaactg accgcaggaa ggcattggca caggtatagc caactgcgga cgccaggccg
      181 cacaatgttc cccacagggg tggatggacc agagagccgg acgctggccc ctgcggcgaa
      241 ggcgatttca cacaccgatt gtagcgaagg accccgctcg atgtcgctcc cttcgatcgc
      301 tcgccgcgtc gcccacctct tggaacaggc cgaacgaaag gtcgtgtttg ccgaaagctg
      361 cactggcggc ctggtgagcg caactttggc ccgcatcgcg ggtatttcga atcacctttg
      421 cggtggcgtg gtcgtttatc gcaacgcgac aaagcaggcc tatctgcaga tcccggctga
      481 cgtgctggag gatccggggc cggtcagcga cgtcgtggcc cgcgaaatgg ctctgcgggt
      541 tctggagaga acgccagaag cggatatcgc ggcatcggtg accggccacc tgggccccaa
      601 cgccccgcgg tatctagacg gggtcgtctt taccggaatt gccgtgcgct cgaccaaccg
      661 acgcgcttcg cagcacgtca aggtgcgccg cctgcgctgc cgcacggccg acgcccgacc
      721 ggcgcggcaa cgatgggtga ccgagcaact gctacaatgg ctcgctgccg aacttgaggc
      781 cctcgcggcc gccccctcgc ccactgggag aggatggggg tgagggcatt gggcaggcca
      841 atcggccagc gagcttccat ggtctttccc ctcagcgacg acaacagcga ccggacgcaa
      901 ttcccggtgg tcacggtcag cctgatcgtg atcaacgtgc tggtgttcgt gctgctacaa
      961 ggggcgggca tgaacgaagg cttcacgatg gccttctcga cggtccccaa agaaatcgtc
     1021 accggtacag acctcgtcac gctcgatcgg cagatagaaa tcgagaccga agaaggtcca
     1081 cagaccgtca ctgcgccggg gctcaagaag acgcctgtct cggtttatct cacgctgctc
     1141 acgagcatgt tcatgcacgg cagcatcatg cacatcgccg gcaatatgtg gtttttatgg
     1201 atcttcggcg acaatatcga gcaggatctg ggccgcatcc ggtacgtgct tttctatctg
     1261 ctcgcgggcc tgctcgcctc gctgactcat gtgctgttca atgccagtgg cccgtctgcc
     1321 gaagtgccca gcctcggcgc ttcgggcgca atcagcggcg tgatgggggc ttacctcgta
     1381 ctgcacccgc atcgccgcgt gacggtcctg ctcttccgtt ttgtgacgca agtccccggt
     1441 tacgtggccg tcggcatctg gttcgcgttc cagatcgtca gcggctggct cgatcggggc
     1501 caaggcggcg ttgcttatgg cgcccacatc ggcggcttca tcgccggcgc ggccctcgcg
     1561 ttccctttca tgctaggccg cccgcgcaag gaaaatcgcg gctacgtcca gaaacgggaa
     1621 tacgacaagg gcatccactg gggtcgctag gcgaccccgg tgtgccacgc cgccggaaga
     1681 aagtgcttgt ttgcgacttc cgcgagggta caatcccggc ttatgcggca tatcggccga
     1741 tagttccctt cgcaatctct cctgcgagat ttctatgtcc acacgatcta ccgccagttg
     1801 gccatttgcc ctggctgccg ctgcggcact tgtcctgctg agcaatactg cgtgggccgg
     1861 cgaagtcggc gtccgcattc gcttcggctt gacggatacg gagccgaccg attggagcgg
     1921 caccgcgacc gccaacccag gcaaggtggt gctcatcagc ggctggcgat tcgagcagga
     1981 cgacgaggcc aagggcaccg aaggctggag cgcctcgacg cgcggcgcgg ccgcgaccgg
     2041 gcgcacgaat gcccaaaagg gcaaagccaa ggcgaaagcc ggagccggcg cagcggctaa
     2101 agcgaaagcc aaggcaaaag ccaaagccgg cgcggcggcg acgatggccg acaacggcgt
     2161 attgctgacg ctggccgacg tgaccgaagc ttcggttgtc aacgtcaaaa ccgaggaagg
     2221 ggattttcag ttcaagattt ccgagattcc ctacggcaaa gt
//
LOCUS       DASUGB010000452         6363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1439753, whole genome shotgun sequence.
ACCESSION   DASUGB010000452 DASUGB010000000
VERSION     DASUGB010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439753"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFV87_08710"
     CDS             complement(<1..415)
                     /locus_tag="VFV87_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103876.1"
                     /translation="MIPRALLAAAMAPVVLCQVHPGEDAGMKPPRIEFVGVAPEFIGE
                     DHVRLAFKAVNPNDAPLPYAGYLATGFRPPLKEGVIAPFYDIEYRRDGTWKAEPIGWC
                     GTGAGPVELPRNGSATFYVSVPKAGWEAMKVAVNWY"
     gene            complement(692..1339)
                     /locus_tag="VFV87_08715"
     CDS             complement(692..1339)
                     /locus_tag="VFV87_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103877.1"
                     /translation="MVGRDVPETIGALFRSIGDLAPQHGIARGELDNAESTLGFKIPV
                     PLRQYYEVIGNHEITWAFNRDYAPNEIECRDGMAVFCEENQCVVYWGFKIEEADLDDP
                     SVWQLNPHENKWYFECKRMSSYLVKSLCWQAACGGLTASASGTIMRDQFAKLGSSFDR
                     IECVEVDDDYDLKAFAGDGIILCAFPDAKKCPVYAGSNDAEKLERLADRFGLDML"
     gene            1518..2549
                     /locus_tag="VFV87_08720"
     CDS             1518..2549
                     /locus_tag="VFV87_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEX5103878.1"
                     /translation="MNTVPVSPLGAAIAGPASGAPAPKKKFLDQCRDVARYRQLSYRT
                     EECYVRWIRQFILHHQKRHPQEMGPEEIREFLTYLAVERHVSASTQNQALGAMLFLYR
                     DVLCRDAGDFAGFAPARRPKRLPVVLTREEVACLLQHLEGTFQLMGQLLYGSGLRLIE
                     CCQLRIKDVDFGRRILTIRDGKGRTDRVTVLPQVLAGPLAEHVARIKRVWEADRRSDL
                     PGVWLPDALDQKYPQAGRSWGWFWVFPARGLSVDPRSGIRRRHHVHENGMQKAMKRAV
                     QRAGIVKHASCHTLRHSFATHLLEGGSDIRTVQELLGHADVSTTMIYTHVLNRPGLAV
                     RSPLDLVGA"
     gene            3090..4043
                     /locus_tag="VFV87_08725"
     CDS             3090..4043
                     /locus_tag="VFV87_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5103879.1"
                     /translation="MSRRKSISRRSFHCHLFAAAATAYGVSAARAAEEFPPGDYVDMH
                     THLGQTWNTTEPLTAEVLLRWMDAHKIAQAVVLPLVSPESSSYLLTSDFVLEQTGPYR
                     DRLIPFCCIDPRTSYSGGHKGLVAMLQKYIDAGAKGFGEHKNGLTFDDPRNMAVYAAC
                     AELKLPLLFHIDNQRNLDKPGLPGLENALKAHPDCNFVGHGPGWWASISGDATDLGGY
                     PKGKVAPGGAIDTLMEKYPNLYGDLSAGSGAGAMSRDLDFAREFLVRRQDRIMFGTDF
                     LAPEQGVPQFELFEQKLADLPPDVKAKIFRDNARKLLGLGK"
     gene            4147..5322
                     /locus_tag="VFV87_08730"
     CDS             4147..5322
                     /locus_tag="VFV87_08730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5103880.1"
                     /translation="MPTLGSWLQDTLFLLSLVNLGFSLFVLVTLSLGDRLIKRLSELP
                     VPELDHWPSVSLIAPGRNEERNIEAAVRSLLELDYPDLEITLVNDRSTDKTGEILDRL
                     AAEFPRLNVVHITELPAGWLGKNHAMQIGAGRSKGEWLLFTDADILFEPTALKRAIGY
                     ALEHQVDHLAATPDTRMPSWLLHAFTITFAIYFSIFFRIWAVRDPKSKAHVGIGAFNL
                     VRAEVYRAVEGHQRIKMRPDDDLKLGKIIKFAGYRQDVVHGSGMIIVEWYHNLSALIR
                     GLEKNAFAGTDYNVFLTVFSTLLSLLFNVWPYIAVFVVPSPTRWIYLTVCLTLWFLAW
                     LIARSMKAPQSCALAFPLTVLLMVYIQWRTMLLNYYHDGIRWRDTHYSLAELRANKV"
     gene            5487..>6363
                     /locus_tag="VFV87_08735"
     CDS             5487..>6363
                     /locus_tag="VFV87_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5103881.1"
                     /translation="MNPRGIAILTLGLLLTFPCLAAGDDWKAGVAKVNITPAESMWMA
                     GYAARTKPAEGKMTDLWAKCLVLEDPAGERAVLVTLDLVGIERQHSQSICQKLQEKYK
                     LDRSQIALNCSHTHSGPVVARNLRPMHFYGLGKDQQQQILKYAEELEAAIVQVVGKAI
                     EGLAPAKIAWGSGKTDFAVNRRNNKEPDVPALREAGQLKGPFDHDVPVLAVRDAAGKL
                     RAVACGYACHATVLSGYDWSGDYPGFAQIELEKAHPDCVALFWAGCGADQNPIPRRAP
                     ELAEKYGKMLADAVEM"
BASE COUNT         1192 a         1936 c         1993 g         1242 t
ORIGIN      
        1 cgtaccagtt caccgcaacc ttcatcgcct cccagcccgc cttcggcaca ctgacgtaga
       61 acgtggccga gccgttcctc ggcaactcga ccgggccggc accggttccg caccagccga
      121 tgggctcggc cttccacgta ccgtcgcggc gatactcgat gtcgtagaac ggggctatca
      181 ctccctcctt cagtggcggg cggaaccctg tcgccaggta cccggcgtag ggcaaaggag
      241 cgtcgttggg gttgaccgcc ttgaacgcga ggcggacgtg gtcctcgccg atgaactcgg
      301 gagcgacgcc gacgaactcg attcgcggcg gtttcatccc cgcgtcctcc ccggggtgga
      361 cctgacagag aacgacaggc gccatcgccg cggccaggag tgctcgtggg atcatcgcag
      421 gtccctttcg aggatgggat cgaccatcct tcagtcataa gccgggcgaa cggtgcctgt
      481 cgaccatttc ccggagtgat gttgcaagcc ggtgacaata cgtggtcggc gcgagtatgg
      541 ctccgtgtga cagtcaacag gatgcgagca cgcctgcctc gccgaacggt tcagttcacc
      601 tgctgcggcg gcggtgagac cttgaacacc ggaaagccgt gatgccgccg cagtcaggtg
      661 caactgatgg ttatggacca tccgatgatt gtcacagcat gtctagtcca aaacgatctg
      721 ccaggcgttc cagcttttct gcgtcattgc tgccggcgta tacggggcac tttttcgcgt
      781 ccgggaaggc gcacaggatg attccgtcgc ccgcaaaggc ctttagatcg tagtcgtcgt
      841 cgacttccac gcattctatc cggtcgaacg acgatccaag cttagcgaac tgatcgcgca
      901 ttatagttcc tgaggcgctt gctgtcaggc cgccacacgc ggcttgccag cacagtgact
      961 tgacgagata cgacgacatc cgcttacatt cgaaatacca cttgttttca tgtggattta
     1021 gctgccacac cgagggatca tccagatcag cctcttcgat cttgaagccc caatagacga
     1081 cgcactgatt ctcttcgcaa aagaccgcca tcccatctcg gcattcgatt tcattggggg
     1141 cgtaatcgcg gttgaaggcc caagtaatct cgtggtttcc aatgacctcg tagtattggc
     1201 gaagcggcac tgggattttg aatccaagtg tgctttcggc attgtcaagt tcgccccttg
     1261 cgatgccgtg ttgaggggca agatcaccga tcgacctgaa caatgcgccg atcgtttccg
     1321 gtacgtcacg cccaaccatg atcagtgtcc atctttggtc cataactctt tattctacag
     1381 aaaacaccca ccgcatagcg cgccagttta tccgtcgaga ggtcgtgact caaggcgcaa
     1441 cgcgaatcgt cgcaatggct tgcttttcgg ctttgccgtg ctatacagaa gtccttctgc
     1501 atagcaaagg actggccatg aacactgtgc ctgtgtcccc gcttggtgct gccattgccg
     1561 gtcccgcttc gggagcgccg gctcccaaga aaaagtttct ggaccaatgc cgtgacgtgg
     1621 ccaggtatcg ccagctctcg tatcgcaccg aggagtgcta cgtgcgctgg attcgccagt
     1681 tcattctgca ccaccagaag cgccatcccc aagaaatggg tccggaggag attcgcgagt
     1741 tcctgaccta tctggccgtg gagcggcatg tctcggcctc gacgcaaaac caagccttgg
     1801 gggcgatgct gttcctgtac cgcgatgtgc tgtgccgcga cgccggcgat tttgctggat
     1861 ttgcccccgc ccgccggccc aagcgcctgc ccgtcgtgct cacgcgggag gaggtggcct
     1921 gcctgctcca gcacctggag ggcacttttc agctcatggg gcaactcctt tacggttcgg
     1981 gcttgcggct gattgagtgc tgccaattgc ggatcaagga tgtcgatttc gggcggcgga
     2041 tactgacgat tcgcgacggg aaggggcgaa ccgatcgcgt gaccgtgctg ccgcaggtgc
     2101 ttgccgggcc tttggcggaa catgtggcgc ggatcaaacg ggtgtgggaa gcggaccgac
     2161 ggtccgacct gcccggcgtg tggctgcccg atgctttgga ccagaagtat ccgcaggcgg
     2221 ggcgttcgtg gggctggttc tgggtgtttc ccgcgcgggg cctgtcggtc gatccgcgga
     2281 gcggcattcg ccgccggcac cacgtgcatg aaaacggcat gcagaaagcc atgaagcgag
     2341 ctgtacaacg ggcaggaatc gtaaagcacg ctagttgcca tacgctgcgg cacagctttg
     2401 ccacgcacct cctggaaggc ggctcagaca ttcgcaccgt gcaagagctg ctgggacacg
     2461 ccgacgtgag cacgacgatg atctataccc atgtgctcaa tcggcccggc ttggccgtgc
     2521 ggagcccgct ggacctggtg ggagcgtagt gccggggcgg gaaccgcgac cactggtcgc
     2581 ggctttgagt cgggcgctcg gcggcggccc gcgccactac gggtagtagg ccgggggaca
     2641 ggcaaccaca agatattgtg gtcttattga cagtagtgtg ctttatgata taatttggag
     2701 tgctgggcgg agggtggctg gcggacgctg gcggtggtcg ttgaacaggg aggctgaacc
     2761 tcagacggga cgggcgtttt atgcatcact tgcataaaat gcataaaccc ctactgggtt
     2821 tcgctggcgc aagctgttgc cgtgacaggg gttgcggcga ccggtgcccg ccgggagaat
     2881 tgtgcgggcg tatgcatgaa tgtattgtgc gcggtgtgcg gcaaggcgtc gcgactgggg
     2941 cacggcgagt acgccttagc cccaggtacc tgccgggttg cggattgacg gggagggtca
     3001 gttgtcagta gtacgttgtc agttggtgaa tggtgggtga gcctgtactc ggtacttcgt
     3061 actgagtact cagtactgag tacttcaaga tgagcagacg caaatcgatc tcgcgacgca
     3121 gctttcattg tcacttgttc gccgccgcgg caaccgcgta cggcgtctcc gccgcaaggg
     3181 cggccgagga gtttcccccc ggcgactatg tcgatatgca cacgcatctg gggcaaacgt
     3241 ggaacaccac ggagccgctg acggcggagg tgctgctgcg gtggatggat gcccacaaaa
     3301 tcgcccaggc ggtcgtgctg ccgctggtgt cgcccgaatc gtcgagctat ctgctgacga
     3361 gcgatttcgt cctggagcag acggggccct atcgcgaccg gctgattccg ttttgctgca
     3421 tcgacccgcg cacgagctac agcggcggcc acaaggggct cgtcgcgatg ctgcagaagt
     3481 atattgacgc gggggccaag ggtttcggcg agcacaagaa cgggctcacc tttgacgacc
     3541 cgcggaacat ggccgtctat gccgcctgcg ccgagctgaa gctgccgctg ttgtttcaca
     3601 tcgacaatca gcggaacctc gataagccag gactgccggg gctggaaaat gccctcaagg
     3661 cccatcccga ttgcaacttc gtcggtcacg gccccggctg gtgggcgtcg atcagcggtg
     3721 acgcgaccga cctgggcggc tatccgaaag gcaaggtcgc gcccggcggc gcgatcgaca
     3781 cgctcatgga gaagtacccc aacctctacg gcgatctctc cgccggcagc ggcgccggag
     3841 ccatgagccg cgatctggat ttcgcccgcg aattcctcgt gcggcggcag gaccggatta
     3901 tgttcggcac cgacttcctc gcgccagagc aaggcgtgcc gcaattcgag ctcttcgagc
     3961 agaagctggc cgacctgccc cccgatgtga aggccaagat tttccgcgac aatgcccgca
     4021 agctgcttgg cctcggcaag taggttccga cggccgcggc ggaacctgaa ccgcccagaa
     4081 catcaactcg cggcaccggt cccgcccagg cggtcgggac ctacataacg cttggatgca
     4141 ccgctcatgc ccacgcttgg gtcctggctg caggacactc tgttcctgct gtcgctcgtc
     4201 aacctgggct tttcgctgtt tgtgctggtg acgctatctc ttggcgaccg gctgatcaag
     4261 cgcctgtcgg aactaccggt ccccgaactg gaccactggc ccagcgtctc gctgatcgcc
     4321 cccggccgca acgaggagcg caacatcgaa gccgcggtcc gctcgctgct ggagctcgat
     4381 tatccagacc tggaaatcac gctcgtcaac gaccgctcga ccgacaaaac gggcgaaatc
     4441 ctggaccgcc tcgcagccga gtttccgcgg ctgaacgtcg tgcatatcac cgagctgccc
     4501 gccggctggc tggggaagaa ccacgcgatg caaatcgggg cgggccgctc caagggcgaa
     4561 tggctgctct tcaccgacgc cgacatcctc ttcgagccga cagcgctgaa gcgggcgatc
     4621 ggctacgcgc tggagcacca ggtcgatcat ttggcggcaa cgccggacac gcgaatgccc
     4681 tcgtggctcc tgcacgcgtt caccatcacg ttcgccatct atttctcgat ctttttccgc
     4741 atctgggccg tccgcgatcc caagagcaaa gcccacgtcg gcatcggggc gttcaacctg
     4801 gtgcgggccg aggtctatcg agccgtcgaa ggccaccagc ggatcaaaat gcggcccgac
     4861 gacgacctga agctgggcaa gatcatcaag ttcgccggct accggcagga cgtggttcac
     4921 ggctccggga tgatcatcgt cgagtggtat cacaacctca gcgccctgat ccgcggcctg
     4981 gaaaagaacg cgttcgcagg caccgactac aacgtgttcc tgaccgtctt ctcgaccttg
     5041 ctgtcgctgc tgttcaatgt ctggccgtat atcgccgtat tcgtcgtccc gtcgcccacg
     5101 cggtggatct atctgacggt ctgtctgacc ctgtggtttc tggcctggct gatcgcccgc
     5161 agcatgaaag ccccgcagtc gtgcgccctg gctttcccgc tcacggttct cttgatggtc
     5221 tatatccagt ggcgaacgat gctgctgaac tactaccatg acggcatccg ctggcgcgac
     5281 acgcactatt cgctggcgga attgcgggcg aataaggtgt gagcgagtgc ggagtgcgga
     5341 gtgcggaatg cggaatgcgg agtctgggga agcggcgatt gcaaagtgct aattgcaaat
     5401 tgaagagcag acgcgcgcca tccaccgaca cagtttgcac tttgcatttt gaaattggaa
     5461 atttgcgatc aacatcgagg tgtttcatga atccgcgcgg gattgccatc ctcacgctcg
     5521 gcctgctgct gacgtttcct tgtctggctg cgggcgatga ctggaaagcc ggcgtcgcca
     5581 aggtcaatat cacgcccgcc gagtccatgt ggatggccgg ctatgcggcg cgcacgaagc
     5641 cggcggaagg aaaaatgacc gacctgtggg ccaagtgcct ggtgcttgaa gacccggcgg
     5701 gcgagcgggc ggtgctggtc acgctggacc tcgtcggcat cgagcggcag cattcgcagt
     5761 cgatctgcca gaagctccag gaaaagtaca agctcgaccg ctcgcagatc gcgctcaact
     5821 gctcgcacac gcacagcggc ccggtcgtcg cccgcaatct gcggccgatg cacttctatg
     5881 gcctgggtaa agaccagcaa cagcagattc tgaaatatgc ggaggaactg gaagcggcca
     5941 tcgtgcaggt ggttggcaag gcgatcgaag gcctcgcccc cgccaaaatc gcgtggggca
     6001 gcggcaagac cgacttcgcc gtcaaccgcc gcaacaacaa ggagccggac gtgccggcgc
     6061 ttcgcgaggc cggccagctc aagggaccgt tcgaccacga cgttccggtc ctcgccgtaa
     6121 gggacgcagc gggaaagctc cgcgcggttg cctgcggcta tgcgtgccat gcgacggtcc
     6181 tctccggcta cgactggtcg ggcgattacc cgggtttcgc gcagattgag cttgaaaagg
     6241 cccatcccga ttgcgtcgcc ctgttctggg ccggctgcgg ggccgatcag aatcccatcc
     6301 cgcggcgggc gccggaactg gcggagaagt acggcaagat gctggccgac gccgtcgaaa
     6361 tgg
//
LOCUS       DASUGB010000453         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_886405, whole genome shotgun sequence.
ACCESSION   DASUGB010000453 DASUGB010000000
VERSION     DASUGB010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886405"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /locus_tag="VFV87_08740"
     CDS             <1..2360
                     /locus_tag="VFV87_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5103882.1"
                     /translation="AGWNYIVFEFIEGTNLRDLVDEHGPLSLEDALNYTLQIAEALAH
                     SSSRDVVHRDIKPSNVLVTAGGQAKLVDMGLARLHQVESSSDDLTASGVTLGTFDYIS
                     PEQARDPRAADVRSDIYSLGCTLFYLLAGQPPFPEGTALQKLLRHNDEERPDVRQFRE
                     DLPPPVIGMLAKMLAKRPSQRYQTPNELIAELVILGRELGMTSITQRGHAVLPAYRAA
                     ADWRMAAWQIGAAVLLLAAAVAIVESWLSAGDEQLNVRMQPTLSAAAEPAVPADDSPT
                     PGKLPVEPRPAGTPVSPAPATASPQAPSTAQARPGAANVEAAGAATPAVESVPASSGV
                     TPPIENVASAEIAPIPIATHESAITPPVGAATIEFAPAAAGLFTSEQSAGLSSPNLPR
                     KISRLVVAPEPPAVVPQDVEYVTTLAEACKRAAELALGEIELQWNGRQVESPLDIASQ
                     RMTLRASAGYQPVVVFQPAVTGLESDRRMVRLLGGSSARLAVAGVELRLELPTEVSYG
                     WSLFSIQTGQSLELADCVLTVQNPDQRHTQVAMIDVRARRVSDTMTMGPPQPAMVSRT
                     RLSLDRCVARGEAAVIAMAEETPITVRWNQGLVATPAHFMETGGTVSNPTSFEPIYID
                     LTRVTAWCGQGVYQMRRRPGAAYQLSVDARADHCIFKTDADAPLYEFIGVPAVAEQDL
                     QFDGEYNLYTDPEVIFLRRRGDRPGDPVENVQIKQPRLLWSQERNLQAGRPWIEAPDL
                     TIPAYRLTTADFAVDEGLSNGAGFDEATLPDMSPPPAPDATDSSP"
BASE COUNT          426 a          838 c          820 g          421 t
ORIGIN      
        1 cggccggctg gaactacatt gtcttcgagt tcattgaagg gacgaacctg cgcgacctgg
       61 tggacgagca cggtccgctg tcgctggaag acgccctgaa ctacacgctg cagattgccg
      121 aggccctggc ccattcctcc agccgcgacg ttgttcatcg cgacatcaag ccctcgaacg
      181 tgctggtcac cgcgggcggc caggccaagc tggtcgatat ggggctggcc cggctgcacc
      241 aggtggagtc gtcgtccgac gacctcacgg ccagcggcgt gacgctcggg acgttcgact
      301 acatctcgcc cgagcaggct cgcgaccccc gcgcagccga cgtccgcagc gatatctatt
      361 cgctgggctg cacgctgttc tacttgctcg ccggccagcc gccgtttccc gaagggaccg
      421 cactgcaaaa gctcttgcgg cacaacgacg aggagcggcc cgatgtgcgg cagttccgtg
      481 aagacttgcc tccgccggtc atcggcatgc tggccaagat gctggcgaag cggccctcgc
      541 agcgctacca gactccgaac gagctgatcg cggagcttgt gattcttggc cgcgagctcg
      601 ggatgacgag cattacgcag cgcggccacg cggtgctgcc tgcctaccgg gcggccgcgg
      661 actggcgcat ggctgcgtgg cagattggag ccgcggtgct cctgctcgcg gcagcagtgg
      721 cgatcgtcga atcgtggctc tcggcgggcg acgagcaact gaacgtgcgc atgcagccga
      781 cgctttccgc cgcagcggag ccggctgtgc cggctgacga ttcaccaacg ccggggaaat
      841 tgccggtcga gccgcgcccg gccggcactc cagtgagtcc tgcgcccgcg accgcttctc
      901 cgcaagcgcc atcgaccgcc caggcaaggc ccggcgcggc gaatgtggag gcggctggtg
      961 ctgcgacacc agcggttgaa tccgttccag cttcctcggg cgtcacgccg ccgattgaga
     1021 acgtcgccag cgcggaaatc gcgccgatcc cgattgcgac gcatgagtcg gcgattacgc
     1081 cgcccgtcgg cgccgccacc attgaattcg cgccagcggc agccggtctg tttacgagcg
     1141 agcaatccgc cgggctgtcg agcccaaact tgccgcgcaa aatcagccgc ctcgtcgtcg
     1201 ctccggaacc gccggccgtt gtcccgcagg acgtggagta tgtgacgacg ctggctgagg
     1261 cttgcaagcg cgcggcagaa ctcgccctag gtgagatcga actacagtgg aatggccggc
     1321 aggtcgagtc gcctttggat atcgccagcc agcgcatgac gcttcgcgcc tccgccgggt
     1381 atcagccggt cgtcgtcttc cagccggcgg tcaccggcct ggaatcggat cgcagaatgg
     1441 tgcggctgct gggcggcagt tcagcgcggt tggcggttgc cggcgtcgaa ttgcggctcg
     1501 aactgccgac ggaagtctcg tacggctggt cgctgttctc tattcagacg gggcagtcgc
     1561 tggaactggc cgattgcgtg ctgaccgtcc aaaatcccga ccagcggcac acgcaggtgg
     1621 cgatgatcga cgtgcgggcc cgccgggtca gcgacaccat gacgatgggc cccccgcagc
     1681 cggcgatggt ttcgcggacc aggctgtcgc tcgaccgctg cgtagcccgc ggcgaggcgg
     1741 cggtgatcgc gatggccgag gaaacgccga tcaccgtgcg atggaatcag gggctcgtcg
     1801 ccacgccggc ccacttcatg gaaaccggcg gcacggtcag caatcccacg agcttcgagc
     1861 cgatctatat cgacttgacg cgcgtcaccg cctggtgcgg ccaaggcgtg tatcaaatgc
     1921 ggcggaggcc gggagccgct tatcagcttt ccgtcgatgc ccgggccgac cactgcatct
     1981 tcaagaccga tgccgacgca cccttgtacg agttcatcgg tgtcccagcc gtggcagagc
     2041 aagacctgca attcgacgga gaatacaacc tctataccga cccggaagtg atcttcctgc
     2101 gccggcgcgg cgatcgcccg ggggatcctg tggagaatgt tcagatcaaa caaccacgcc
     2161 ttctctggtc ccaggaacgc aacctccagg cgggaagacc ttggatcgaa gcgccggacc
     2221 tgacgattcc cgcctatcgc ctgaccactg cggatttcgc ggtggatgaa gggctttcca
     2281 acggggccgg attcgacgag gccacgctgc cggacatgtc gccgcccccc gcgccggacg
     2341 ctacggattc ttcaccgtag gcatctcgct ccgcgagatg caatggactt ggcgacgcgc
     2401 acaagccctc gcgcttggag actcgtccgc tactcgcgga gcgagtcgct tacggcgtcg
     2461 ccgctaggat gacacctgcg ctgcgcctag aatggccgtc tgcgt
//
LOCUS       DASUGB010000454         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1997796, whole genome shotgun sequence.
ACCESSION   DASUGB010000454 DASUGB010000000
VERSION     DASUGB010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997796"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFV87_08745"
     CDS             complement(<1..449)
                     /locus_tag="VFV87_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103883.1"
                     /translation="MIAAGSARHSGGRLAGRWISLGLALCLALPLAVRAETPVNPLRP
                     NHAPIVTPKKSPTLADPALKPADYHAGTQLDDVWSQPAPALARSVVAANGEAPEPPSI
                     VPPQFGEPEALPPPSIRYDMPPDAGYDPHVGGWQDHYVHHWFDEPWFS"
     gene            1472..2086
                     /locus_tag="VFV87_08750"
     CDS             1472..2086
                     /locus_tag="VFV87_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103884.1"
                     /translation="MRCGGLFWRFIPVAVWAISGCGARVRPPAFSPSAASAAAIKQLD
                     TSGSDQLEAEELRQAPGLAAALDRADADKSGTLSQGEIRDRIASYGASRVGLMPFTCT
                     VRLDGRELAGAEVKLVPEAFLASALKPAIATTSETGVAAPVVEGEQFAAMPCGMYRVE
                     ISQRDAAGAQIVPARYNVRTELGQEIAPDVPGLERGVVFELVTQ"
     gene            2126..3091
                     /locus_tag="VFV87_08755"
     CDS             2126..3091
                     /locus_tag="VFV87_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103885.1"
                     /translation="MIETFSDSGYCQFPRRRGFTLIELLVVIAIIGILIALLLPAVQA
                     AREAGRRMQCQNHLKQIGLAFHNHHDVLKHYPTGGWGWNWVGDPDLGFAQKQPGGWTF
                     NILPYIEGKTIYDMGRGQPGPTKQAQLAAMVGTPIKYYYCPSRRQPAIYPITVLPVNS
                     DPVTRGAKLDYAVNCGDQDRNEWAGDAPPNMEPPTNYTFTGIVFCCSTIEMTQVIDGT
                     SNTIMVGEKYLNPLNYANGVDAADNENLYVGFDNDNARSTHANYFPPRPDTVGLTLHI
                     FGSAHPGTFNVVLCDGSVRGLSYRIGPVPYQRLGNRLDGQPVNLD"
     gene            3176..>3393
                     /locus_tag="VFV87_08760"
     CDS             3176..>3393
                     /locus_tag="VFV87_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103886.1"
                     /translation="MLNWPLILDQTAFGFLIVSRNGKMSQILNQIELRPNREGRLRAY
                     IGGTRVRVQDVYALAELQQKSPDEIVIAL"
BASE COUNT          627 a         1107 c         1063 g          596 t
ORIGIN      
        1 gagaaccagg gctcgtcgaa ccagtgatgc acataatgat cctgccagcc gccgacgtgc
       61 ggatcgtagc cggcgtccgg cggcatgtcg tagcggatgc tgggcggcgg caaagcttcc
      121 ggctcgccga actgcggcgg gacgatcgac ggcggctcgg gcgcttcgcc attggccgcg
      181 acgaccgagc gggccagcgc cggcgcaggc tgcgaccaga cgtcgtcgag ctgcgtgccc
      241 gcgtgatagt cggccggctt gagtgccggg tcggcgagcg tggggctttt cttgggggtc
      301 acgatcggcg catggttcgg ccgcagcgga ttgacgggcg tctcggcgcg gaccgccagc
      361 ggcaaggcca ggcaaagcgc caggcccagg ctgatccagc gaccggccag ccggccgccg
      421 gagtgccgag cactccccgc ggcaatcatt ttgaccatcc cgcaaaagaa gaggggcgga
      481 ttattccaag gtgttcgatc caggtcaacg gaaagcggag agcccttctc ccgctccccc
      541 tcgtggggag agggaccagg gtaagaagcc gcgcagcgcg caactttcgc atattttttc
      601 acgcagcacc tccccgcgca acattcacct aaatcccatt cccacaaaca cttgcgcagc
      661 gcaacacccc ccacatgagg atgttgcgct gttgcgcatt gtcaaccatt ttttctctgt
      721 tttttgaccc atcgactggc atatggcgcc agaccccctg cctcctcact cccgtcctca
      781 atctccctct ccctctggga gagggccagg gggtgagggt gctttccggc ccacagacac
      841 gcgcgcacac catcacctgt atagatgcgc taacccgccc aattatttcc ctcgcccgaa
      901 aaaaatcttg gaccaaaaac ccagcagccg catggaagtt tgcttccccg agccgagtca
      961 gtaaactact gcccagcaaa tgatcgccca gccgcgtcag cgtggagacg gtcaagatcg
     1021 tcaacgccct acccgcgcag tttgagccaa tgtaggcgag cttcgcctca cgtctccctg
     1081 ggccgcggca ctctttcaga gtgccgggca atgacgcgac tcattcccca gggctgcgtt
     1141 ggccagcgcc aacttgccct gggctaacca gttttggcct ttcaggccag ggaattcaag
     1201 cgttctcgct cacgctcgcc ggcattccga ccgaggtgtt cgactaccgc ctcggcaacc
     1261 gctcggccct cgaatgggtc atcgaccaat accaggtcaa caccgacaag cgcagcggca
     1321 ttacctctga tcccaaccga cgccctcccc gcggagttcg agccagagta gccctgtcag
     1381 gctcgcgctg gctttggagc gggagtttgg ttagcttgtg gggcagggct tggcgcggtg
     1441 gcgctgcttg ggggaaagca gggggcgggt catgcgatgc ggggggctgt tctggcgatt
     1501 tattccggtg gcggtttggg cgatttccgg ttgcggggct cgggtccggc cgccagcgtt
     1561 cagcccctcc gcggcgagcg cggcggcgat caagcagctc gatacgagcg gcagcgacca
     1621 gctcgaagcg gaggagctgc gacaagcacc gggcctggct gccgcactcg atcgggccga
     1681 cgccgacaag agcggcacgc tgagccaggg ggagattcgc gaccgcatcg cgagctacgg
     1741 cgcgtcgcgg gtcggcctga tgccgttcac ctgcaccgtc cggctcgatg gccgcgagct
     1801 ggcgggggcc gaggtcaagc ttgttccgga ggcgttcctc gcatcagcgc tcaagccggc
     1861 catcgccaca acgagcgaaa cgggcgtcgc ggcgccggtc gtcgaaggcg agcaattcgc
     1921 cgccatgccc tgcggcatgt atcgcgtgga aatctcccag cgggacgccg ccggcgcaca
     1981 aatcgtgccc gccaggtaca acgtccgcac cgagctgggc caggagatcg cgcccgacgt
     2041 gccggggctg gaacggggcg tcgtgtttga acttgtcacc cagtgaggtt accatctaag
     2101 ccatcttcaa tggggagttc tcctcatgat cgagaccttc agcgattcag gctactgcca
     2161 attcccgcgc cgccgcggct tcacgctcat cgagctgctg gtggtcattg cgatcatcgg
     2221 cattctgatc gcgctgctct tgccggcggt gcaggcggct cgcgaagcgg ggcggcggat
     2281 gcagtgccag aaccacctca agcagatcgg cctggccttt cacaaccacc acgacgtgct
     2341 caagcactat cccacgggcg gctgggggtg gaattgggtc ggcgatcccg acttgggctt
     2401 cgcccagaag cagcccggcg gttggacctt caacatcctg ccgtacatcg agggcaaaac
     2461 catttacgac atgggccgcg gccagccggg gccgacgaag caggcccaac tggcggcgat
     2521 ggtgggcacg cccatcaagt attactactg cccttcgcgg cggcagccgg cgatttatcc
     2581 gatcacggtc ctgccggtga attccgaccc ggttacgcgg ggcgcgaagc tcgactatgc
     2641 ggtcaactgc ggcgaccagg accgcaacga atgggcgggc gatgctccgc cgaacatgga
     2701 gccgcccacg aactacacct ttaccggcat cgtcttctgc tgcagcacga tcgagatgac
     2761 gcaggtgatc gacggcacgt cgaacacgat catggtcggc gaaaagtacc tcaacccgct
     2821 caactatgcc aacggcgtcg atgccgccga caacgagaac ctgtacgtcg gcttcgacaa
     2881 cgacaatgcc cgctcgacgc acgccaatta ctttccgccg cggcctgaca cggtggggct
     2941 gacgctgcac atcttcggca gcgcccatcc cgggacgttc aacgttgtgc tctgcgacgg
     3001 ctcggtccgc gggctctcct accgcatcgg ccccgtcccc taccagcggc tgggcaaccg
     3061 cctcgacggg cagccggtca atttggattg acggcgcccc cgatcgacaa agacgggccg
     3121 aggatgtggc gctagttccc tctgccggac gttgctaggt taaggcgagg ggagcatgtt
     3181 gaactggccg ttgattctgg accaaacggc gtttgggttc ctgattgttt cccgaaacgg
     3241 caaaatgagt caaatcttga accaaatcga gttgcgtccc aaccgtgagg gacgcctgcg
     3301 agcctatatc ggcggaacgc gcgttcgcgt gcaggacgta tatgcgcttg ctgaactcca
     3361 gcaaaaatcg ccggatgaaa tcgtgatcgc cct
//
LOCUS       DASUGB010000455         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1581710, whole genome shotgun sequence.
ACCESSION   DASUGB010000455 DASUGB010000000
VERSION     DASUGB010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581710"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="VFV87_08765"
     CDS             complement(<1..1985)
                     /locus_tag="VFV87_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5103887.1"
                     /translation="MIPITHTTPFRPKGHRLLPALLCGLLLMAATRTAAAEGADGLRL
                     FRERIEPVLKRHCYECHSAEAKQLQGGLRLDSRLGMLRGGDSGPAISPGKSRQSLLIQ
                     AIRHEGDYQMPPDKPKLPEAVIADFARWVDAGAADPRADEPQAAAADPRRHWAFQPVR
                     KPALPAVNDVAASRWPMDTFVLAKLDERGWQLSPPAERTQLLRRAAFDLIGLPPTPDE
                     IDAFGRDPAPDAYERAVDRLLASPHYGERQAQHWLDVVRFAETEGYEYDRHVPGAWRF
                     RDYVIDAFNRDKPYDRFITEQIAGDEIAPQDHECLTASIFHRLGPVRRNAGNPEIALS
                     RNEVLTERTDIVGTAFLGLSIGCARCHNHKLEPISQRDYYSLQAYLAATDEHNISLAS
                     AAEQAAWEAETATIQRQIDRLKQEMKGASLADKTRIAAEIEALDEKLPPPLPTIPATW
                     NDHTKRTAIHVLRRGVWEDKGEAVGPRPPSVLIPEEEEALAADASNPRTRLAEWIASP
                     EHPLTARVFVNRLWQQHFAAGLVATANDFGLRGERPSHPELLDWLAAEFVENGWQIKP
                     LQRAMVLSATYRQGSSAEFGMRNAKWETASISATPHSALRTPHSSDPDNRLLWRFPRR
                     RLSAEELRDAMLAVAGRLNLESGGPSIMPPVDPQLVDL"
     gene            2154..3422
                     /locus_tag="VFV87_08770"
     CDS             2154..3422
                     /locus_tag="VFV87_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103888.1"
                     /translation="MHSISSQRFDRRSFLSAAAAATAGTLLSPSVSRGQASPVRETDQ
                     FWFRLAPEGPYIDCQREHKAFGFGDGKVFLSEDCGKSWAHSADFPDAKNITFSCLLDN
                     GNILFATRQKLFLSTDNLKTVREIIVKDRDGSDYVPHSPQDPDQPGWYFHPLDGIHTW
                     DFDGSEMLVWGNYCNVLGGPVPVNIYYSTDGGQTVKIAYSFGRNPHFQEKGTAPEAFL
                     GDPANPVICRHIHAVTYNPAERAFYACTGDINRGHGNECHWLRGQYDARSDKWDWQVI
                     ISVNSNSRYKSGGINFVDGQLYWAADANGPKPADQKHDRGIFRCNPAELADPAKHTLL
                     FNAEFEMANMIIEDGVILAGHCAPASTYKTGIAFSPDLGKTWAEYDLAELGPRSPVRF
                     QPKNSDGWFRVDLRKGWIERAEVLFIKPKV"
BASE COUNT          622 a         1166 c         1133 g          633 t
ORIGIN      
        1 agatcgacaa gctgcggatc gacgggcggc atgatgctcg ggccgccgga ctccaggttg
       61 agccggccgg cgacggcgag catggcgtcg cgcagctctt ccgccgaaag gcggcgccgc
      121 gggaagcgcc agaggaggcg gttatcaggg tcggacgaat gcggagtgcg gagtgcggaa
      181 tgcggagtgg cggaaataga agctgtctcc catttcgcat tccgcattcc gaattccgca
      241 ctcgagccct gccgatatgt cgcgctcagc accatcgccc gctgcagcgg cttgatttgc
      301 cagccgtttt ctacgaactc ggcagccagc cagtcgagca gctccggatg gctcggccgc
      361 tcgccgcgga ggccgaagtc gttcgccgtg gcgaccagcc cggctgcgaa gtgctgctgc
      421 cacaggcgat tcacaaagac gcgggcggtg agcggatgct cgggcgaggc gatccactcc
      481 gccaggcgcg tccgcgggtt gctggcgtcc gctgcgagtg cttcttcctc ctccgggatc
      541 agcacgctcg gcggacgcgg gcccaccgct tcgcccttgt cttcccacac gcctcgccgc
      601 aggacgtgaa tggccgtgcg cttcgtgtga tcgttccacg ttgccggaat cgtcggcaag
      661 gggggcggca gcttctcgtc gagcgcttcg atctcggcgg cgatccgcgt tttgtcggcc
      721 aggctggccc ccttcatctc ctgcttcagc cggtcgatct gccgctggat cgtcgcggtc
      781 tcggcttccc aggccgcctg ctcggcagca gaggcgagcg agatattgtg ctcgtcggtc
      841 gctgccagat acgcctgcag ggagtaataa tcccgctggg agatcggctc gagcttatgg
      901 ttgtggcagc gggcacagcc gatggacagg cccagaaacg ccgtgccgac gatgtcggtc
      961 cgctcggtca gcacctcgtt gcggctgagg gcgatctcgg gattcccggc gttgcggcgg
     1021 accggcccca ggcgatggaa gatgctggcc gtcaggcatt cgtgatcctg cggggcgatc
     1081 tcgtcccccg caatctgctc cgtgataaac cggtcatagg gcttgtcgcg gttgaaggcg
     1141 tcgatcacgt agtcgcggaa tcgccacgcc cctgggacgt ggcggtcgta ctcgtagcct
     1201 tcggtctcgg caaagcgaac cacgtccagc caatgctgcg cctgccgctc gccatagtgt
     1261 ggactggcca acagccgatc gacggctcgc tcgtaggcgt ccggcgccgg gtcgcgcccg
     1321 aaagcatcga tctcgtccgg cgtcggcgga aggccaatca gatcgaacgc agcgcgccgc
     1381 agaagttggg tccgctcggc gggcggcgaa agctgccagc cgcgctcatc gagcttcgcc
     1441 aggacgaacg tatccatcgg ccagcgagag gcggcgacat cgttcaccgc tggcagggcg
     1501 ggcttcctca ccggctggaa agcccagtgc cggcgcggat cggctgcggc agcttgcggc
     1561 tcgtccgcgc gcgggtcggc tgcgccggca tcgacccaac gcgcgaagtc tgcaatcacc
     1621 gcctctggca gcttcggctt gtcgggcggc atctgatagt cgccctcgtg gcgaattgcc
     1681 tggatcaaga ggctctggcg gctcttgccg ggcgaaatcg cgggcccgga atcgccgccg
     1741 cggagcatcc ccagccgcga gtcgagccgg aggccgccct gcagctgctt cgcctccgcc
     1801 gagtggcact cgtagcagtg ccgcttcagc accggctcga tccgctcgcg aaacagccgc
     1861 aggccatcgg cgccttcagc cgccgctgtt cgcgttgctg ccatcagcag cagcccgcac
     1921 agcagcgctg gcaacaagcg atgccctttc ggtcgaaacg gtgtggtgtg tgtgatgggg
     1981 atcatcagga gctgccaaca cgcgtcgcca aagaatgact cactgccatg cgactatatt
     2041 acccggttgg cctgccagat cgctatgctt ggcgaatgac ttggaagcca aacggctgcg
     2101 gatgaactac cataacgact gcagtcccct gcctccggct gaaggaagtt ctcatgcatt
     2161 cgattagttc ccagcgattc gaccgccgtt cgtttctctc agccgccgcg gcggccaccg
     2221 cgggaacttt gctgagcccg agcgtttccc gcggtcaggc ctcgccggtg cgcgaaaccg
     2281 accagttctg gtttcgactg gcgcccgaag gcccctacat tgactgccag cgcgagcaca
     2341 aggccttcgg ctttggcgac ggcaaggtgt ttctgtcgga agactgcggc aagtcgtggg
     2401 ctcacagcgc ggactttcct gacgcgaaga acatcacctt cagttgcctc ttggacaacg
     2461 ggaacatcct gtttgccacg cggcagaagc tcttcttgag caccgacaat ctcaagactg
     2521 tgcgagagat tatcgtcaag gatcgggacg ggagcgacta cgtgccgcac tctccccaag
     2581 acccggatca gccgggctgg tacttccatc cgctcgacgg cattcacacc tgggactttg
     2641 acgggtcgga aatgctcgtg tgggggaatt actgcaacgt gctcggcggt ccggtgccgg
     2701 tcaacatcta ttactcgacc gacggcggcc agacggtcaa gatcgcctat tccttcgggc
     2761 ggaacccgca cttccaggag aagggaacag ctcccgaggc gttcctgggc gatcctgcga
     2821 atcccgtgat ctgccgccac atccacgccg tcacctacaa ccctgcggag cgggcgttct
     2881 atgcctgcac cggcgacatc aaccgcgggc acggcaacga atgccactgg cttcgcggcc
     2941 agtacgacgc ccggtccgac aagtgggact ggcaggtgat tatctccgtc aactcgaatt
     3001 cgcgctacaa gtcgggcggc atcaacttcg tcgatgggca gctctattgg gcagccgacg
     3061 ccaacggccc caagcccgcc gaccagaagc acgaccgcgg catttttcgc tgcaatcctg
     3121 ccgagctcgc cgatccggcc aagcacacgc tgctgtttaa cgcggaattc gaaatggcca
     3181 acatgatcat cgaggacggc gtgatcctgg cggggcactg cgctcccgcc tccacgtaca
     3241 agacgggcat cgccttttct cccgacctgg gcaaaacctg ggcggaatac gacctcgccg
     3301 aactcgggcc acgctcgccc gtgcgtttcc agccgaaaaa cagcgacggc tggttccgcg
     3361 tggacctgcg caagggatgg attgaaagag ccgaagtgct gtttatcaag ccgaaggtct
     3421 gagaggcaaa aatccatgcc atacacacca aagctctcgc ccaccgttcc cgcggctgta
     3481 ttccttgcag ccttggtggc ggcggccagc gccgacgaag tcgcgaactg gccgcggttc
     3541 cgcgggaaca atgg
//
LOCUS       DASUGB010000456         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_470132, whole genome shotgun sequence.
ACCESSION   DASUGB010000456 DASUGB010000000
VERSION     DASUGB010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470132"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFV87_08775"
     CDS             <1..624
                     /locus_tag="VFV87_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5103889.1"
                     /translation="VCDVEFEDQIARLRDELAAAGHKFAGLLHSIAFADYADGMKPFH
                     ETPKRAFLRAMDISCYSLTAVSNALKDLLEPEASVVTVSISTTRMASENYGYMAPIKA
                     ALDSSLAFLAKSFSQFSRVRFNAVAPGLLKTSASAGIPGYVDSYLYAEQVIPRRAAVE
                     TQEAANVAAFLLSPRSSGINAQVVVVDAGMSINYFDAAVIKRAMRPE"
     gene            complement(599..1735)
                     /locus_tag="VFV87_08780"
     CDS             complement(599..1735)
                     /locus_tag="VFV87_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HEX5103890.1"
                     /translation="MRYQNVCLEALGYAIPDEVVTSDEIERRLEPLYRRLKLPEGRLE
                     LMSGIRERRLWPRGFQPSDGSIASGRRAIDAAGIDPREIGCLIHGSVCRDQLEPATAC
                     RVHDALGLPANCLAYDVSNACLGMLSGVVQVANLIELGQIRAGLVVGAEDSRALLEST
                     IAALNADQALTRDSVKPAFASLTIGSASAAVLLVHREISRAGNALQAAAARAHTAHHQ
                     LCQGGHEAGSNGSSGILMQTDSEELLKQGLAAGAATFSDFLQTSGWSRESTHKSICHQ
                     VGGTHRRLMLESLGLPADRDYATFPWLGNTGSVALPIALALAAENAFVEPHDHVALLG
                     IGSGINCLMLAVNWQRTLAASGVGFQPAAPHYLGNRATPAASPA"
     gene            1894..>3172
                     /locus_tag="VFV87_08785"
     CDS             1894..>3172
                     /locus_tag="VFV87_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX5103891.1"
                     /translation="MGFQIHQPFPPAGDQPQAIARLVEGIQAGKRAQCLLGVTGSGKT
                     FTMANVIAHFDKPTLVISHNKTLAAQLYGEFKEFFPRNAVHYFVSYYDYYQPEAYIPQ
                     RDIYIEKDASINEELDRLRLATTSSLVSRRDVIIIASVSCIYGLGSPQDYKSMMVPLR
                     VGETLDRDEMLMRLVDIQYDRNDVAFERGKFRVRGDCVEIWPSYEEYAFRLELWGDEV
                     EKLSIINPISGETLATEEQLFIYPAKHFVMPEDRIAQAIDTIRQELDERLKILREAGK
                     LLEAQRLSARTRFDIEMLMETGHCPGIENYSRPLSGRPPGSTPDTLHDFFPDDFLLIV
                     DESHVTIPQIRAMYNGDRARKEVLVEHGFRLPSAMDNRPLKFEEWERKKAQIVFVSAT
                     PGEYELAKTGGEVVEQIIRPTGLLDPVIEVVPAR"
BASE COUNT          594 a          971 c         1048 g          559 t
ORIGIN      
        1 gtgtgcgatg tggagttcga ggaccagatc gcccgactgc gcgatgagct ggcggcagcc
       61 gggcacaaat tcgcggggct gctgcactcg atcgccttcg ccgactacgc ggacggcatg
      121 aagccgtttc acgagacgcc gaagcgggcc tttttgcggg cgatggacat ctcgtgctat
      181 tcgctgaccg cggtcagcaa cgcgctgaag gacctgctgg agccggaggc gtcggtggtg
      241 acggtttcga tcagcacgac gcggatggcg agcgagaact atggctacat ggcgccgatc
      301 aaggcggccc tcgattcgtc gctggcgttt ctggcgaaga gcttcagcca gttttcgcgc
      361 gtgcgattca acgcggtcgc gccgggtcta ttgaagacga gcgcgtcggc tgggattccg
      421 ggctacgtcg attcgtacct gtatgccgag caagtgattc cgcgacgggc ggccgtggaa
      481 acgcaggaag cggcgaatgt ggcggcgttc ttgctgagcc cgcggtcgag cggcatcaac
      541 gcgcaagtcg ttgtggtcga tgcggggatg agcatcaact atttcgacgc ggcagtgatc
      601 aagcgggcga tgcggccgga gtagcacggt taccgaggta gtgcggagcg gcaggctgga
      661 agcctacccc actggcggcg agcgtccgct gccaattaac tgcgagcatc aggcaattga
      721 tcccgctgcc gatgccgagc agggcgacgt gatcgtgcgg ctcaacgaaa gcattttcgg
      781 ccgcgagggc cagcgcgatc ggcagggcga ccgagccggt gttgccgagc cagggaaatg
      841 tggcgtaatc gcgatcggcg ggcaggccga gcgattccag catcagccgg cggtgcgtgc
      901 cacctacctg atggcagatg gacttgtgag tcgattcgcg cgaccagcca ctggtctgga
      961 ggaagtcgct gaatgtcgcc gcgccggcgg cgaggccctg tttgagcaat tcttcggagt
     1021 ccgtctgcat cagaattccc gaactgccat tggaaccagc ctcatgaccg ccctgacaaa
     1081 gctgatgatg agccgtgtgc gctcttgcgg ccgcggcttg cagggcgttt ccggcccggc
     1141 tgatctcgcg atgcacgagc agcacggcgg cgctcgcgga gccgatcgtg agcgaagcga
     1201 atgccggctt cacgctgtcg cgggtcagcg cctggtcggc gttgagcgca gcgatcgtgc
     1261 tttcgagcag cgcccggctg tcttccgcgc cgacaacgag gcccgcgcga atctgcccca
     1321 gctcgatcag gttcgctacc tgcacgacgc cgctcagcat gccgaggcaa gcgttcgata
     1381 cgtcgtacgc caggcaattc gccggcaatc ccagcgcgtc gtgcacgcgg caggccgttg
     1441 ccggctcaag ctgatcgcgg cagaccgagc cgtggatcag gcaaccgatt tcgcggggat
     1501 cgatgcccgc cgcgtcaatc gctcgccggc cgctggcgat gctgccgtcg ctcggctgaa
     1561 atccgcgcgg ccacagccgc cgctcgcgga tgccgctcat cagctccagc cgcccctccg
     1621 gcagcttcag ccggcgatac agcggctcca atcgccgttc gatctcgtcg ctggtgacga
     1681 cctcgtccgg aatcgcatag ccgagcgctt cgaggcagac gttctgataa cgcatggcgt
     1741 tcagcataga aatggccgga caaatcgtaa acgggagcgc gcaccgccat ccggaccaag
     1801 aaccgcagtt tcagcccgtt gttcaccgct ttccccgcat accgtgacct gcgtacagta
     1861 gaatttggac tctcgccaca tcggacgagc tttatgggct tccaaatcca ccaaccgttc
     1921 cctcccgctg gcgatcagcc gcaggcgatt gcccggctgg tcgaagggat ccaagccggc
     1981 aagcgggcgc agtgtttgct cggcgtgacc gggtcgggca agacgttcac gatggccaac
     2041 gtcatcgccc acttcgacaa gccgacgctc gtcatctcgc acaacaaaac gctggcggct
     2101 cagctttatg gcgaattcaa ggagttcttt ccgcggaacg ccgtccacta cttcgtcagc
     2161 tattacgact actaccagcc ggaagcgtac atcccgcagc gcgacatcta catcgagaag
     2221 gatgcctcga tcaacgagga gctcgaccgg ctgcggctgg cgacgaccag ctcgctcgtc
     2281 agccggcggg atgtgatcat tatcgccagc gtgtcgtgca tttacggttt gggctcgccg
     2341 caggactaca agtcgatgat ggtcccgttg cgggtcggtg agacgctcga ccgcgacgag
     2401 atgctgatgc ggctggtcga tatccagtac gaccgcaacg acgtggcatt cgagcggggc
     2461 aagtttcgcg tccgcggcga ctgcgtcgaa atctggccga gctacgagga atacgccttc
     2521 cgcctggaat tgtgggggga cgaggtcgag aagctctcga tcatcaaccc gatcagcggc
     2581 gagacgctgg cgaccgaaga gcagctcttc atctatccgg ccaagcactt cgtgatgccc
     2641 gaagatcgca tcgcccaggc gatcgacacg attcgccagg agctggatga gcggctgaag
     2701 attctgcgcg aagcgggcaa gctgctggag gcgcagcgct tgagtgcgcg aacgcggttc
     2761 gatatcgaaa tgctgatgga aacgggccac tgtccgggga tcgagaatta cagccggccg
     2821 ctttcggggc ggccgccggg ctcgacgccc gatacgctgc acgacttctt cccggacgac
     2881 ttcctgctga tcgtcgatga gtcgcacgtg acaatcccgc agatccgagc gatgtacaac
     2941 ggcgaccggg cccgcaagga agtgctcgtc gaacatggct tccggctgcc aagcgcgatg
     3001 gacaaccggc cgctgaaatt cgaggaatgg gagcggaaaa aggcgcagat tgtgttcgtg
     3061 agcgctacgc cgggcgagta cgagttggcg aagacgggtg gcgaagtcgt cgagcagatc
     3121 atccggccga cggggctgct cgatccggtg atcgaggtcg tccccgcccg cg
//
LOCUS       DASUGB010000457         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_607518, whole genome shotgun sequence.
ACCESSION   DASUGB010000457 DASUGB010000000
VERSION     DASUGB010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607518"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="VFV87_08790"
     CDS             <1..1805
                     /locus_tag="VFV87_08790"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX5103892.1"
                     /translation="SLLATRFRTKDLLNIADVYARNASELFSLEMWGGATFDTALRFL
                     KEDPWQRLALLRERIPNILFQMLFRASNAVGYASYPDNVVREFCQEAADAGMDIFRIF
                     DSLNWVPNMKVAMEAVLKTDAICEPAICYTGDILNPERSKYDLKYYVELAKELEELGA
                     HILAIKDMAGLCKPYAAQLLVKTLKEEIGIPLHFHTHDTAGMQVGSILEAAEQKLDIA
                     DGAMAAMSGGTSQPNLNALVEALRFTKRDTGLEGERLAEISDYWQAVREFYLPFDSTL
                     LPATAELYSHEMPGGQYTNLYQQARALGLADRWREVCRAYADANQMLGDIVKVTPSSK
                     AVGDLALFMVANSLSAQEIAEGDRELAFPESVIDLLSGMMGQPPGGWPKGLQKRILKD
                     RAPVKGRAGASLPAADFDEAAEKVRGMITREPNRRDCLSYLLYPKVYQEFAAHVRKYS
                     DTSNLPTPVFFYGQETGEEFSIDIEEGKTLIIKFLTVSEPHADGTRTVFFELNGQPRD
                     VSVVDLSLEATAAKAIQADPADPKQVGAAMPGMVVTVTVKAGDKVKKGQKLFTVEAMK
                     METTVNSEVEGRVAEVLVKPGSRVEAGDLLLRIE"
     gene            1860..2636
                     /locus_tag="VFV87_08795"
     CDS             1860..2636
                     /locus_tag="VFV87_08795"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5103893.1"
                     /translation="MFDVTRIQRSGLVARVDFHESLGSTSDWALKLAAAGESPLPLLV
                     LAEKQTDGRGRGTNRWWSADGALTFSLIMEASPESLAPNRWPQISLSAGLAVCEMLEE
                     FLPRHDCRIKWPNDVFVGDRKICGILCESAPGWRDRIVLGIGLNVNNSLAHAPLEVRQ
                     TAVSLIDAAGGAYDRTGVLLSLLDRFEQRWRQLIAGQFVDQTAAFRQRCFLTGKMLTI
                     VCGSKTHVGRCQGIDDAGELLLQTESGLGRFVAGTIVVCE"
     gene            2707..>3006
                     /locus_tag="VFV87_08800"
     CDS             2707..>3006
                     /locus_tag="VFV87_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5103894.1"
                     /translation="MPRPFCLFIAACLLSLFLLGCGAMEQAREAAKRQQTMNHLKQLG
                     LAYHNCHDATGKGPANWQEAVQFSLPSDAQAALEGAGYTVHWGIKLADATGGTASF"
BASE COUNT          590 a          887 c          979 g          550 t
ORIGIN      
        1 agtcgctctt ggccacgcgg ttccgcacga aagacctgct caatatcgcc gatgtctatg
       61 cccgcaatgc cagcgagctg ttttcgctgg aaatgtgggg cggggcgacg ttcgacacgg
      121 ccctgcggtt cctgaaggag gatccctggc agcgattggc cctcttgcgt gagaggattc
      181 caaacatcct cttccagatg ctcttccggg ccagcaatgc cgtcggctac gcgagctatc
      241 ccgacaacgt cgtccgcgaa ttctgccagg aagcggccga tgcgggcatg gacatcttcc
      301 gcatcttcga ttcgctcaac tgggtgccga acatgaaggt cgccatggag gcggtcctca
      361 agacggatgc gatctgcgag ccggccatct gctatacggg agatattctc aacccggagc
      421 gatcgaagta cgacctcaaa tactacgtcg agctggccaa ggagctggaa gagctcggcg
      481 cgcacatcct ggcgatcaag gacatggccg ggctgtgcaa gccctatgcg gcccagctct
      541 tggtgaagac gctgaaggaa gagatcggca tcccgctcca cttccacacg cacgatacgg
      601 ctggcatgca ggtcggctcg atcctggagg cggcggagca aaagctcgat atcgccgacg
      661 gagcgatggc ggccatgtcg ggcggtacca gccagccgaa cctgaacgcc ctggtcgagg
      721 ccctgcggtt caccaagcgc gacacgggct tggaaggcga aaggctggcg gaaatctcgg
      781 attactggca ggccgtccgc gaattctacc tgccgttcga cagcacgctc ctaccggcga
      841 cggcagaact gtacagccac gagatgcccg gcgggcaata taccaatctc tatcagcagg
      901 cccgtgctct ggggctggca gatcgctggc gagaggtctg cagggcctac gccgacgcga
      961 accagatgct gggcgacatc gtcaaagtga cgcctagcag caaggcggtg ggagatctgg
     1021 ccctcttcat ggtggccaac agcctatccg ctcaggaaat cgccgagggc gaccgcgagt
     1081 tggcgtttcc cgagtcggtg atcgacttgc tttccggcat gatggggcag ccgcccggcg
     1141 gctggccgaa gggcctgcag aagcgaatct tgaaggaccg cgcacccgtg aagggccggg
     1201 ccggtgcgag cttgccggcg gccgactttg acgaagcagc cgagaaggtt cgcggaatga
     1261 tcacccgcga gcccaaccgc cgcgattgcc tttcttatct gctgtatccg aaggtctatc
     1321 aggagtttgc cgcccacgtt cgcaagtact ccgatacgag taacttgccg acgcccgttt
     1381 tcttctacgg ccaggagacc ggcgaggaat tcagcatcga tatcgaagaa ggcaagacgc
     1441 tgatcatcaa gtttctcacc gtcagcgagc cgcacgccga cggcacgcgg accgtctttt
     1501 tcgagctgaa cggccagccg cgggatgtta gtgtggtcga tctctcgctc gaagccaccg
     1561 ccgccaaagc catccaggcc gatcccgccg atcccaagca ggtgggcgcg gcgatgccgg
     1621 gcatggtggt gacggtgacc gtgaaggccg gcgacaaagt gaaaaagggc cagaagctgt
     1681 ttaccgtcga agccatgaag atggagacga cggtcaatag cgaggtcgaa ggccgtgtgg
     1741 ccgaggtcct ggtcaagccc ggcagccgcg tggaagcggg cgatttgctc ctgcggatcg
     1801 agtgagggtc ggggcaagcg gtcagaggtt agaagtcaga ggtgggcgag aattcctcga
     1861 tgtttgacgt gacccggatt caacggtcgg ggcttgtggc tcgcgtggac tttcacgagt
     1921 cgctgggctc gaccagcgat tgggcgctga agctggctgc ggcaggcgag tcaccgctcc
     1981 cgctcttggt gctggccgag aagcagaccg acggccgagg gcgagggacg aatcgctggt
     2041 ggtcggccga cggcgcgctg acgttttcgc tgatcatgga ggcttccccg gaaagtctcg
     2101 cgccgaatcg ctggccgcag atttcgctgt ccgccggttt ggccgtttgc gaaatgctgg
     2161 aagagttttt gccgcgacac gattgccgca tcaaatggcc caatgacgtg tttgtcggtg
     2221 accgcaagat ttgcggcatc ctgtgcgaat cggcgcccgg ctggcgggac cggatcgtgc
     2281 tggggatcgg cctgaatgtc aacaattcgc tcgcgcatgc gccgctggaa gtccggcaaa
     2341 cggccgtgtc acttatcgat gccgccggcg gcgcttatga tcggacgggc gtgttgctgt
     2401 cgctgctcga ccgattcgaa cagcgctggc ggcaactgat tgccggtcaa ttcgtcgatc
     2461 agaccgctgc ttttcgccaa cgatgcttcc tgaccggcaa aatgctgacc atcgtctgcg
     2521 gcagcaagac gcacgtcggc cgctgccaag gaatcgacga cgcgggcgaa ttactgctgc
     2581 aaaccgaatc ggggctgggc cgcttcgtcg cggggacaat tgtcgtctgt gagtgattgc
     2641 cagcttccgg cgcggtgctt aaagtagcaa aaaactcccc cccgcttcac tcgcttggag
     2701 ggcacaatgc cacgaccgtt ctgcctgttt atcgccgcct gcctgttatc gctgttcctg
     2761 ctgggctgcg gcgcaatgga acaagcccgt gaggccgcca aacgccagca gacgatgaac
     2821 catctgaagc agctagggct ggcttaccac aattgccatg acgccacagg gaagggaccc
     2881 gcgaattggc aagaagcggt gcagttcagc ttgccgtccg acgcgcaagc ggcgctcgaa
     2941 ggggctggct atacggtgca ctggggcatc aagttggcgg acgccacggg aggaaccgcc
     3001 agtttt
//
LOCUS       DASUGB010000458         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1440707, whole genome shotgun sequence.
ACCESSION   DASUGB010000458 DASUGB010000000
VERSION     DASUGB010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440707"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFV87_08805"
     CDS             <1..351
                     /locus_tag="VFV87_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103895.1"
                     /translation="LQGHIEAGPFAVGGLALFSAEPEGLLCVESGPKIRWQRPLAHGP
                     IAGPPLPLADGDLLVIYQSGVVSRVSAETGEEVGMPSNVAQPLGAAASVLGQNVFVSG
                     SDGVIHRAALPQRP"
     gene            364..2736
                     /locus_tag="VFV87_08810"
     CDS             364..2736
                     /locus_tag="VFV87_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5103896.1"
                     /translation="MLLPGQQRLAQQRWLPTAALAWIAALAICHALADSRPALAQQPK
                     RLFEQDPFDIVTLTETNESKVLKVYPLALPGRKIPEKPKASERLRIKLVEDGSEYDVA
                     WNDIAQVELFEQMVLAEANRIVGEGKFDEAYDYFAFLLQFYPNMPGLAEGRDAYLYLA
                     AGSAFRQRKYDEALAVLEELLARNPNYRAGENSATLLQVLGNIADPILAAYVAQADYR
                     SARILLNRLVRQYSAADQPFAQRWRERMTEMATKHRDEAKAHLAAGRFVAAKDAAAAM
                     VQIWPDLEGASELFAEIARTYPLVVVGVEHPAKSYDPRSLHDFAARRTGRLVERLLVE
                     FEGPGPEGGRYSSPLAGVSTSDDGLSLSFRLPANAAASQPSAFGLAQRLVTLARPASD
                     EFRPAWAHILQSVRTDGPRNVVADLRLPYVLPEALLQAPLAPRGAEGGSPGAASGPFV
                     FLSQEESLGRFTANADYPFRRPGQLAEVAERYYDDPQRLLIALKQGEVDLVDRVFPGD
                     IGVLAADSNVVVAPYAAPTTHVLAIRGGHPYLANRTFRRALLYATNRELILKQGLLRG
                     RDVPGFRLVSGPFPASAPGLNLPGYGYDETIEPREFDPRLALTLRLLGQGEVKGAYEK
                     QKQTVPPLTALLLGHPADETSRVACKALVKQWKTIGVDCKLVEFAPGIFDDESGKCDL
                     VYLQLAAWEPIVDAARLLGPDGPAFAPSDFVQLTLRQLANAQNWQQGRERLLRLHRLI
                     HEDVALLPLYQTIDHYAYRRTLQGPSQPRVTLYQDIDQWQTAPQVAEARP"
     gene            2877..>3079
                     /locus_tag="VFV87_08815"
     CDS             2877..>3079
                     /locus_tag="VFV87_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103897.1"
                     /translation="MIAAVPGMCRGQAAWEYSPYQVRIWVALETTPQLPPELISPLSE
                     SLTARADSVMGAVWSVEVQPVPVA"
BASE COUNT          507 a         1026 c         1000 g          546 t
ORIGIN      
        1 ctacaggggc acatcgaagc cggccccttt gccgtgggcg ggctggcgct gttttcggca
       61 gagcctgaag gtctgctgtg cgtggaatcc ggccccaaga tacgctggca gcggccactg
      121 gctcatggtc cgatcgccgg acctccgctg cccctggccg atggcgactt gctggtgatt
      181 tatcagtcgg gcgttgtcag ccgggtctct gccgagacag gcgaagaagt gggcatgccg
      241 agcaatgtcg cccagccgct cggtgctgcc gccagcgtcc tggggcaaaa cgtcttcgtg
      301 tcgggcagcg acggcgtgat tcaccgcgcc gccctgccgc agcgtcccta aatgccctcg
      361 tccatgctgc ttccaggtca acagcgattg gcgcagcagc gctggctccc cacggcggcg
      421 ctggcttgga tcgccgcgct cgccatttgc cacgcccttg ccgattcgcg gcctgccttg
      481 gcccagcagc ccaagcggct gttcgagcag gacccgttcg acatcgtcac gctgacggaa
      541 acgaacgaga gcaaggtcct caaggtctat ccgctggctc ttcccggccg caagattccc
      601 gagaagccga aagccagcga gcggttgcgg atcaagctcg tcgaggacgg aagcgagtac
      661 gacgttgcct ggaacgatat tgcccaagtc gagctgttcg aacagatggt cctggccgag
      721 gccaaccgca tcgtcggcga gggcaaattc gacgaggcct acgactactt cgcgtttctg
      781 ctgcagtttt atccgaacat gcccggcctg gccgaaggcc gagatgcgta tctgtatctg
      841 gcagccggtt ccgcctttcg tcagcgcaag tacgacgaag cgctggccgt cctcgaggag
      901 ttactggcgc ggaatccgaa ctaccgcgcc ggtgagaact cggccacgct gctgcaagtg
      961 ctgggcaata tcgcggaccc gatcttggcg gcctatgtgg cccaggcgga ctatcgctcg
     1021 gcccggatac tcttgaaccg ccttgtgcgg caatatagcg ccgccgacca gccgttcgcc
     1081 cagcgctggc gcgagcggat gacggaaatg gcaacgaagc atcgcgacga ggccaaggcc
     1141 cacctcgccg cgggccggtt cgtcgccgcc aaagatgccg ccgccgcgat ggtgcagatc
     1201 tggcccgatt tggagggcgc cagcgagctg ttcgcagaaa tcgcccgcac gtatccgctg
     1261 gtcgtcgtcg gcgtggagca tccggcaaaa agctacgatc cgcggagcct gcacgacttc
     1321 gccgccaggc gaaccggccg tctggtggag cggctgctcg tcgagttcga ggggccgggg
     1381 ccggaaggtg gccgctacag ctcgccgctc gccggtgttt cgaccagcga cgacgggctc
     1441 agcctcagct tccgccttcc cgccaatgcc gcggcttcgc agccaagcgc ctttggcctc
     1501 gcccagcggc tggtgacgct cgcgcggccg gcgagcgacg aatttcgacc ggcctgggct
     1561 catattctac agtcggtgcg gaccgacgga ccgcgcaacg tcgttgccga tttgcgcctg
     1621 ccgtatgtat tgccggaagc actgctgcaa gcgccgctgg cgccgcgcgg cgctgagggc
     1681 ggatcgcccg gggcggcgag cggcccgttc gtatttctct cgcaagagga atcgctgggc
     1741 cggttcacgg ccaacgcaga ctatccgttc agacggcccg gccagctggc ggaagtcgcc
     1801 gaacgatact acgacgatcc gcagcgcttg ctgatcgcct tgaagcaggg cgaggtcgat
     1861 ctggtcgatc gcgtcttccc cggcgacatc ggcgtgctgg cagccgattc gaacgtcgtc
     1921 gttgctccct acgccgcgcc caccacgcac gtgctggcga tccgcggcgg gcacccgtat
     1981 ctggccaatc gcacgtttcg ccgggcgctc ttgtacgcga ccaatcgcga gctgatcctc
     2041 aagcaggggc tgctccgcgg cagagacgtt ccgggctttc gccttgtcag cgggccgttt
     2101 cccgcgtccg cgcccggtct taatctcccc ggctacggat acgacgagac cattgagccc
     2161 cgcgaattcg acccgcgatt ggcgctgacc ttgcggctgc tgggccaggg cgaggtgaag
     2221 ggcgcttacg agaagcagaa gcaaacggtg ccgccgctga ctgccctgct gctgggccat
     2281 cccgccgatg aaacatcgcg cgtcgcgtgc aaggccctcg tcaaacagtg gaagacgatc
     2341 ggcgtcgatt gcaagctcgt cgaatttgca ccgggcatct tcgacgatga aagcggcaag
     2401 tgcgacctcg tatatctgca actcgccgcc tgggagccga ttgtcgatgc cgcccgcttg
     2461 ctgggtccgg acgggccggc gtttgcaccc agcgatttcg tgcagctcac gctgcggcag
     2521 ctcgccaatg cgcagaactg gcagcaaggc cgcgagcgct tgctgaggct gcaccgcttg
     2581 attcatgagg acgtcgccct gctgccgctc tatcagacga tcgaccacta cgcttatcgc
     2641 cgcacgctgc aaggcccgtc gcagccgcgg gtgacgctct atcaggacat cgaccagtgg
     2701 cagacagccc cgcaggttgc ggaggcccgg ccatgagcgg aataccttcg aagtggaccc
     2761 gcggagcttc tcctgcccta ggacaggttt ccaacctgtc cgaggcggac gggttgaaaa
     2821 cccgtcctac gtctgcgaga ccccgcactt ggcttgtagc gctatacgct gtggccttga
     2881 tcgcggcggt gcccggcatg tgccgcggac aggccgcctg ggaatattcc ccctaccagg
     2941 tgcggatctg ggtcgcgctg gaaacgacgc cgcagttgcc gcccgagctg atttcaccgc
     3001 tttccgaatc gctgacggcc cgtgcggact ccgtgatggg cgctgtctgg tcggtcgaag
     3061 tccagccggt cccggtggc
//
LOCUS       DASUGB010000459         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1164265, whole genome shotgun sequence.
ACCESSION   DASUGB010000459 DASUGB010000000
VERSION     DASUGB010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164265"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VFV87_08820"
     CDS             complement(<1..1430)
                     /locus_tag="VFV87_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5103898.1"
                     /translation="MASLATDFDPYRTWLGIQEPHRPLNPYQLLGLVPLETDVARIRA
                     GFERQQAALAMWAGKADSELWETVQGELAQAYDLLGDAEQKAVLDAGIRRRTGGAKTA
                     NGKPSPATGAAATVACRQCQRQNPTGRRFCSGCGKPLWEQCPQCCAECAADEQFCGHC
                     GADIRGGLAEQRRQLEERIEQALEMAAGHRYDAAVSALRGVAAVSDPRMEHFASRALE
                     EITKIECDHKTFQRAAEDGLGQARRFMESYAYENAQHAIEDVPEPLRTQEHRDILLRA
                     KSARQELLSLGGEIRQAVEQKKTWDLLPKLERLLALKPNHGQARELAEVLRDNLVKTA
                     KTRLGEHRYREALDSLDQIPSFVRNSEVSTLSEAATELLSLLEAVRNAALADRQLQGL
                     ADRLCKLAASNAEAAKLRAQLMEKLKGRPANRRLGAPNWTEPPKRTRIGVPIDWLAHL
                     TRPRPADDSVAEALDDHPGQVFTAFGL"
     gene            complement(1542..1970)
                     /locus_tag="VFV87_08825"
     CDS             complement(1542..1970)
                     /locus_tag="VFV87_08825"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5103899.1"
                     /translation="MKHNVLFLCTGNSCRSQMAEGLLRNLASDRFEVYSAGTHPAERV
                     HPQAVNVMAEEGIDISGQQPTPVQKYMGRLQFTHMMIVCDGANEECPRIFPGVLHRHF
                     WPFDDPAKLTGSEAEVLAGFRRVRDEIREKIENWLAEMTS"
     gene            complement(1975..>2804)
                     /locus_tag="VFV87_08830"
     CDS             complement(1975..>2804)
                     /locus_tag="VFV87_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103900.1"
                     /translation="KELDPGEETFVSLDWNTGRSLVYSHSATIFTNDAARRSIQFTVT
                     GKVLLLIGADVEQISLPPAESGSKREVELLLHSQIWKDFEVVSADLPVEDFVWRVEDV
                     DPKAVPSLDAEGVRKLHMEFHTPQARDFQHTLQLKVAQADVAGEPESLSIPISGSVLR
                     PLSFYGPDIDSEGTIDLGNIPEGESRRAKLIAKVRDEIRTLDNAKVEISPDFVRATFE
                     PYPSGQPGIYQLSVEVPAGQPPCQYKSNPTGRLTIHTEHPRIGDVELLVTFAIVPRS"
BASE COUNT          491 a          897 c          847 g          569 t
ORIGIN      
        1 aggccaaacg ccgtgaagac ttgccccgga tggtcgtcga gcgcttccgc gaccgaatcg
       61 tctgccggcc tggggcgtgt caaatgggca agccaatcga tggggacacc gatgcgcgtc
      121 cgcttgggcg gctcggtcca attcggcgcg cccaggcggc ggttagcggg cctgcccttc
      181 agcttctcca tgagttgcgc ccgcagtttg gccgcttccg cgttgctggc ggccagtttg
      241 cacaggcggt cggccagacc ctggagctgc cggtcggcca aggcagcgtt tcgcaccgct
      301 tccagcaggc tgagcagctc cgttgccgct tcgctgagcg tgctcacttc gctattgcgg
      361 acgaacgagg ggatttgatc gagtgaatcg agagcttcgc gatagcgatg ctctccgagg
      421 cgggtcttgg cggtcttcac cagattgtcg cgcagcacct ccgccagttc gcgggcctgg
      481 ccgtggttcg gcttcagcgc cagcaggcgt tccagcttgg gaagcagatc ccacgtcttc
      541 ttttgctcga cggcctggcg aatttcgccg cccagggaga gcagctcctg acgagcggat
      601 ttcgcccgca agaggatgtc gcggtgctcc tgcgtccgca gcggttcggg cacgtcctcg
      661 atcgcgtgct gcgcgttttc gtaggcgtac gactccatga accgccgcgc ctggccgagc
      721 ccgtcttccg cagcccgctg gaaggtcttg tggtcgcatt cgatcttggt gatttcttcg
      781 agggcccggc tggcgaaatg ttccatccgc ggatcggaca ctgccgctac gccgcggagc
      841 gcggagacag ctgcgtcata ccggtgtccg gcagccattt ccaaggcctg ctcgatccgc
      901 tcttcaagct ggcgccgctg ttcggccagg ccgccgcgga tgtcggcgcc gcagtgaccg
      961 cagaattgct cgtcggccgc gcattcggcg cagcactggg ggcattgctc ccacagcggc
     1021 ttaccgcagc cgctgcagaa ccgccggcca gtcgggttct gacgctggca ctgccggcag
     1081 gccaccgtcg ccgctgcgcc cgtggccggt gagggtttgc cgttcgccgt cttcgctccg
     1141 ccggtgcgcc gccggatccc cgcgtcgagc acggctttct gttccgcatc acccagcaag
     1201 tcgtaggcct gcgccagttc gccttgcacg gtctcccaaa gctcactgtc cgccttgcca
     1261 gcccacatcg ccagggctgc ctgctgacgc tcaaaaccgg cgcggatcct tgcgacatcc
     1321 gtctccagag ggacaagccc cagaagctgg tagggattta gcgggcggtg cggttcctga
     1381 atgccgagcc aggttcgata cggatcgaaa tctgttgcca aactggccat gcgacgcgat
     1441 gggtgcggag gatgcggatc gtcaccgccc aaactcgccc gcaaccatta agaaactgca
     1501 aagactatgt gaagtttggt tccagcgcga cgagccgaag ctcaactcgt catttcggcc
     1561 agccagtttt cgatcttctc tcgaatctcg tcccgcaccc gccgaaagcc agccaacact
     1621 tccgcctcac tgcccgtgag cttggccggg tcgtcgaacg gccagaaatg ccgatgcagc
     1681 acgcccggaa agattcgcgg gcactcctcg ttcgcgccgt cgcagacgat catcatgtgc
     1741 gtgaattgca gccggcccat gtatttctgc acgggcgtcg gctgctggcc agagatgtcg
     1801 atgccctctt cggccatcac gttgacagcc tgaggatgca cccgctcagc cggatgcgtg
     1861 ccggcgctat agacctcgaa ccggtcactc gccaaattcc gcaagagtcc ttccgccatc
     1921 tggctgcggc aggagttgcc cgtgcacagg aaaagcacat tgtgcttcat ggagtcacga
     1981 tcgagggacg atggcaaacg ttaccaacaa ttccacgtca ccaatccgcg gatgctccgt
     2041 gtggatcgtc aatcggcccg ttggattgga cttgtactgg cacggtggct gtcccgctgg
     2101 gacttcaacg gaaagctgat agatgcccgg ctgaccactt ggatagggtt cgaaagtcgc
     2161 ccgaacgaag tcgggtgaga tctcaacctt cgcgttgtcc agcgttcgga tctcatcgcg
     2221 aactttggcg atgagcttgg ctcgtctcga ctccccctcg ggaatgttgc ccaggtcgat
     2281 tgtgccttcc gagtcgatgt ccggcccata aaatgacagg ggtcgcaaca ccgacccgct
     2341 aatcggaatc gatagagatt cgggctcgcc ggcaacgtcg gcctgagcga ccttcaactg
     2401 caatgtgtgc tgaaaatcac gcgcctgcgg agtgtggaac tccatgtgca gcttcctgac
     2461 gccttcggcg tcgagactgg ggaccgcttt cggatcgacg tcttcaacgc gccacacgaa
     2521 atcttccaca ggcaaatcag cgctgactac ttcgaagtcc ttccagatct gtgaatgcag
     2581 cagcagttca acttccctct tgcttccgga ttccgcaggc ggaaggctga tctgttccac
     2641 gtcggcgccg atcagcagca ggaccttgcc agtgaccgta aattgaattg aacggcgggc
     2701 ggcgtcgttc gtaaaaatgg tggccgagtg ggagtaaact agactgcggc ccgtattcca
     2761 gtcgagcgaa acaaacgttt cttcccctgg gtccaattcc ttct
//
LOCUS       DASUGB010000460         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_331098, whole genome shotgun sequence.
ACCESSION   DASUGB010000460 DASUGB010000000
VERSION     DASUGB010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331098"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFV87_08835"
     CDS             <1..731
                     /locus_tag="VFV87_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5103901.1"
                     /translation="KEFLAETIKAFSPDLLGTQETLGFQRDFLAGQLADYGVVGVGRD
                     DGQEQGEMMALYFRRERFEKLGEGHFWLSETPDVSGSKSWDSSLPRMVTWVKLQDRQQ
                     PGQPPLWLFNTHFDHRGPQARLHSAELLRKQIGLLAKGDPVIVTGDFNSGEASEPYRA
                     LFAAAGGEDSPVIDCFRAAHPERGASEGTASGFEAKNTGGARIDWIACSRHWEVRQAE
                     IVRAAREGRTPSDHFPVTVVLQRK"
     gene            complement(736..1272)
                     /locus_tag="VFV87_08840"
     CDS             complement(736..1272)
                     /locus_tag="VFV87_08840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5103902.1"
                     /translation="MKLACGVALGLFVAAILAGGAAAAELKVGDDAPEFTMVGSDGKT
                     YKLSDFKGKQAVVVAWFPRAFTGGCTKECKAFKEQGSSIREYDVAYFTASNDPAEKNK
                     AFAESLGVDYPILSDADSSVAKAYGVLNDKGTAALRWTYYIGADGKILHIDKSVKTES
                     HGADVAAKLGELGVAKKK"
     gene            1535..2170
                     /locus_tag="VFV87_08845"
     CDS             1535..2170
                     /locus_tag="VFV87_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103903.1"
                     /translation="MQANVAELPLNPYASPPLDEPIIRAELADEKPKPGSLPLIFLGF
                     ILLLVGYLASNIFAIADLYQIGFGPGGEVIPSPLADVFSTPGWQWLFYGICAAATIAG
                     CILIGSQNFNPLVAVIFIMCPLVAVVYLLAMPLRMAQKWTVPMAATYLMIGTCLAGVG
                     ISRLIGLYGQAGLNFEPILASLLTEIGLALLGGAMLKLAFAPSDEPRAAVA"
     gene            complement(2225..2671)
                     /locus_tag="VFV87_08850"
     CDS             complement(2225..2671)
                     /locus_tag="VFV87_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX5103904.1"
                     /translation="MRRYQIWLLLGAVFGCLGVILGAIGAHGLEDYLRVQGGADSTFT
                     EAEIVKLLANWETAARYQMYHALALLAVGFVAWRRCSAGIHVAGAAMTLGTLLFSGCL
                     YVLVLSGEKWLGAIVPIGGLLMIIGWALLLFAVARLEAAAQCGSTP"
     gene            complement(3002..>5212)
                     /locus_tag="VFV87_08855"
     CDS             complement(3002..>5212)
                     /locus_tag="VFV87_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103905.1"
                     /translation="DEKNSEIKADLVRKQELTFEVQNKLAEMVEKFNVLMEEARYAEA
                     EVVAKQANDLVPRDPVVVTMVEKARLARHIAEELSIKAEKEDGVVRALHGISRSAIIN
                     VDDDNPLVFNPKRWEDLSKSKYRMMQEKSRYSPAEIEIQRSLSKPVEVRFENRPLAEV
                     LDALGRMSGVNVHMDEQGLHAEGVSSDTPVTINLTQPISLKSALNLILQPKGLSYVIQ
                     NEVLLITSEQTRDANVYPHVYYVADLVMPIPNFVPSYNLGLPGAIRESLNALGYGGGV
                     RPISAGPLTLAANEQQNLQQQAVSPLAASTNPEILAQAGMSNLIPSVGHSRTPQMTAG
                     PGGLGGGVIADFDSLIELITTTISPDSWDEVGGPGSISGFDTNLSLVVSQTQEVHEQL
                     ADLLEQLRRLQDLQVTIEVRFITLSDSFFERIGVDFQFDIDDNSNLNNFIDLLPTGPA
                     TDSPNFFDDTNPAKVIGLTAAGPTADLDLSFTQGSFASTTPTFGGFDANTAANFGFAI
                     LSDIEVFLLIEAAQGDSRTNILQAPKVTLFNGQQAFVSDTSQRPFVTSVIPVVGDFAA
                     AQQPVIVVLNEGTSLNVQAVVSADRRFVRLTLVPFFSQIGDVDTFTFNGRVTTDSGTI
                     VQDPSDSEQNVNNNNVRTVEGTTVQLPTFAFTTVTTTVSVPDGGTVLLGGIKRLREGR
                     TERGVPLLSKVPWVSRLFKNVGIGRDAQSLMMMVTPRIIIQEEEEAKLGIEVQP"
BASE COUNT          952 a         1651 c         1615 g          994 t
ORIGIN      
        1 gcaaggagtt cctggccgaa acgatcaagg cgttttcgcc cgaccttctg ggcacgcagg
       61 aaacgctggg ctttcagcgc gattttctgg ccgggcagct tgccgattac ggcgtcgtcg
      121 gcgtcggccg cgacgacggc caagagcagg gcgagatgat ggccctgtat ttccggcgcg
      181 agcggttcga gaaactcggc gaaggccact tctggctgag cgagacgccg gacgtgtccg
      241 gcagcaagag ctgggacagc tcactcccgc ggatggtcac gtgggtgaag ctccaggacc
      301 gccaacagcc cggtcagccg ccgctgtggc tgtttaacac gcacttcgac catcgcggcc
      361 cgcaggcgcg gctgcactca gccgagctgt tgcgaaagca aatcggcctg ctggccaagg
      421 gcgatccggt catcgtgacg ggcgacttca actcgggcga agcgagcgag ccttaccggg
      481 cgctgtttgc agcggccggc ggcgaggatt cgcccgtcat cgattgcttc cgcgcggcgc
      541 accccgagcg cggcgccagc gaagggaccg cctcggggtt tgaggccaag aacacgggag
      601 gcgcgcggat tgactggatc gcctgctcgc gccactggga ggtgcggcag gccgagatcg
      661 ttcgcgccgc ccgcgaaggg cggacgccgt ccgatcactt tccggtaacg gtcgtgctac
      721 agcgaaaatg acctgctact tcttcttggc gacgccgagt tcgcccagct ttgcggcgac
      781 atcggcgccg tggctctccg tcttcacgga cttgtcgatg tgcaggatct tgccgtcggc
      841 gccgatgtag taggtccagc gaagggcggc ggtgcccttg tcgttgagca cgccataggc
      901 cttggccacg ctgctgtcgg cgtcgctcag gatcgggtaa tcgacgccca gcgattcagc
      961 gaaagccttg ttcttctcgg ccgggtcgtt gctggccgtg aaataggcca cgtcgtactc
     1021 gcgaatcgag ctcccctgct ctttgaaggc cttgcactcc ttcgtgcacc cgccggtgaa
     1081 agcccgcggg aaccaggcga cgacgaccgc ctgcttgcct ttgaaatcgg acagcttata
     1141 cgtcttgccg tcgctgccga ccatcgtgaa ctcgggcgcg tcatcgccga ctttcaattc
     1201 ggccgcggcc gcgccgccgg ccaggatcgc ggcgacaaac aaacccaggg ccacgccaca
     1261 tgcgagcttc atggacagat ctccaacgag ggaatgagat attaacccga agcgtaagcg
     1321 agggactccc ctctccgata cgcttcgcgc ttggtgtgaa cgcaagccgg taggcgctca
     1381 tcacgcggag cgtgatggct acgagagcca ccttaacata tcgacgttcc ctgattcaac
     1441 atgccggtgg tagcgattat gcggcggcgg ctcttcgatc ggcgatggga cgcgcgacgt
     1501 aggtttgaat ggtcgtccca cccgagagcc aaccatgcaa gccaacgtcg ccgaattgcc
     1561 gctcaatccc tatgccagcc ctccgctgga cgagccgatc attcgcgccg aactggccga
     1621 cgaaaaaccc aagccgggtt cgctccctct catctttctg ggattcattc tgctgctcgt
     1681 cggctatttg gcgtcgaaca tctttgcgat cgccgacctg tatcagattg gtttcggccc
     1741 gggcggagaa gtcattccct cgccactggc tgatgtgttc agcacgccgg gctggcagtg
     1801 gctgttctat ggaatctgcg ccgccgcaac gattgccggc tgcatcctga tcggcagcca
     1861 gaacttcaac cccctggtgg cagtgatctt catcatgtgc ccgctggtcg cagtcgtgta
     1921 cttgctggcc atgccgctgc ggatggctca gaaatggact gtgccgatgg ctgcgactta
     1981 cctgatgatc ggcacctgcc tggcgggtgt cggcatctcg cggctgattg gactctacgg
     2041 ccaggcgggg ctgaacttcg agccgatcct ggccagcttg ttgacggaaa tcgggctcgc
     2101 cttgctgggc ggcgcgatgc tcaagctggc cttcgcgccc agcgacgagc cgcgggcggc
     2161 agtggcctga gaatcgtagg gtgggctatg cccaccagac acaaggcggt gggcacagcc
     2221 caccctacgg cgtcgagccg cactgcgctg cggcctccaa cctggcgacg gcgaagagca
     2281 acagcgccca gccgatgatc atcagcaggc cgccgatcgg cacgattgcg ccgagccatt
     2341 tctcgccgga aagcacgaga acgtagaggc agcccgagaa gagaagcgtg cccagcgtca
     2401 tcgccgcccc cgcgacatgg attcctgcac tgcagcgccg ccaggccacg aatccgacgg
     2461 ccagaagcgc gagcgcgtga tacatctggt agcgggcagc cgtctcccag ttggcaagca
     2521 gcttgacgat ttccgcctcg gtaaaagtgc tgtccgcacc gccctgcacc cgtaaatagt
     2581 cctccagccc atgcgcgcca attgcgccca gaatcacgcc caggcagccg aagaccgcgc
     2641 ccagcagcag ccagatttga tagcgtcgca tgacctcatt ttagtgacga cgctgccgct
     2701 ttgacaccaa cccgacgcgt cagcgaggga actcacacca acccgacgcg ccagcgaggg
     2761 acctcacacc aacccgacgc gtcagcgagg gacctcacaa caacccgaag cgccagcgag
     2821 ggacgccctc gctcacgcgt cgggttacga caagaaacga aagccgccgc aggtccgggg
     2881 ggatggaacc tgcggcggcc ctcagagaag tctggatggg gcggacagac acgaatgtct
     2941 gcccactttt ctggcaggct gaaagcctac cccacgtggc aggctggaag cctgccccac
     3001 gttacggctg aacctcgatg ccgagcttgg cttcctcttc ttcctgaatg atgatccgtg
     3061 gcgtgaccat catcatcagg ctttgagcat cgcgtccgat accgacgttc ttgaacaagc
     3121 ggctgaccca cggcaccttg ctgagcagcg gcacgccgcg ttcggtccgg ccctcgcgga
     3181 ggcgtttgat gccgcccagg agcaccgtcc cgccgtcggg cacgctgact gtcgtcgtga
     3241 ccgtggtgaa ggcgaacgtc ggcaactgga cggttgtgcc ttcgaccgtc cgcacgttgt
     3301 tgttgttcac gttctgctcg gagtccgacg gatcttgcac gatggtgccg ctgtcggtgg
     3361 tgacgcggcc gttaaacgtg aacgtgtcca cgtcgccgat ctggctgaag aacggcacca
     3421 gcgtcaggcg aacgaaccgc cggtccgcgg aaaccaccgc ttgcacgttc aggctcgtcc
     3481 cttcgttgag gacaacaatc acgggttgct gggcggcggc gaaatcgccg acgaccggga
     3541 tcacgctcgt tacgaacggt cgctgcgacg tatcggagac gaacgcttgc tggccattga
     3601 acagcgtcac cttcggcgcc tggaggatgt tcgtgcggct gtcgccctgt gcggcttcaa
     3661 tcagcaggaa cacctcgata tcgctcagaa tcgcaaagcc gaagttggcg gcggtgttcg
     3721 catcgaaccc gccgaacgtt ggcgtcgtcg aggcaaagct cccttgcgta aacgacagat
     3781 cgagatctgc ggtcggtccc gcagccgtca agccgattac cttagccggg ttggtatcgt
     3841 cgaagaaatt cggcgagtcc gtggccggcc cggtgggcaa caaatcgatg aagttgttga
     3901 ggttcgagtt gtcgtcgata tcaaactgga aatcgacgcc gatccgctcg aagaacgaat
     3961 cgctgagcgt gatgaaccgc acctcgatcg tcacctgcaa atcctgcagg cggcggagct
     4021 gctccagaag gtcggccaat tgctcgtgga cttcctgcgt ctggctgaca accaggctca
     4081 agttggtatc gaaacccgat atcgaacccg gcccgcccac ttcgtcccag ctatcgggcg
     4141 agatcgtcgt ggtgatcagc tcgatcaggc tgtcgaagtc ggcaatcacg ccgccgccca
     4201 agccgcccgg tccggcggtc atctgcggcg tgcgcgagtg gccgacgctg ggaatcaggt
     4261 tgctcatgcc cgcctgggcg aggatttccg ggttggtgct cgccgccagg ggcgagaccg
     4321 cctgctgctg caggttttgc tgctcgttgg cggcgagcgt cagcggcccg gccgaaatcg
     4381 gacggacgcc gccgccgtag cccagagcat tgaggctctc gcggatcgcg ccgggcaagc
     4441 ccaggttata gctgggcacg aagttcggaa tcggcatcac caggtcggcg acataataga
     4501 cgtgcggata gacatttgcg tcgcgcgtct gctcgctggt gatcaggagc acttcgttct
     4561 gaatcacata gctcagcccc ttgggctgca gaatcagatt cagggcgctc ttcagcgaaa
     4621 tgggctgcgt gaggttgatc gtcaccggcg tatcgctgct gacgccttcg gcgtgcagtc
     4681 cctgctcgtc catgtgaacg ttcacgcccg acatccggcc cagcgcatcg agcacttccg
     4741 ccagtgggcg attctcgaac cgcacttcga cgggcttgga gagcgagcgc tgaatctcga
     4801 tttctgccgg cgaataccgc gacttttcct gcatcatgcg gtacttgctc ttggacaagt
     4861 cttcccagcg cttggggttg aagacgaggg ggttatcgtc atcaacattg atgatggccg
     4921 acctcgaaat cccatggagt gcccgaacaa cgccatcttc cttttcagct ttgatggaga
     4981 gctcctctgc gatgtgtctc gcaagtcgcg ctttctccac catggttacg acgaccggat
     5041 cgcgcggcac gaggtcgttc gcctgtttgg cgacaacttc ggcctcggcg taccgggctt
     5101 cttccatcag cacgttgaac ttttccacca tctcggccag cttgttctgg acttcgaagg
     5161 tcagctcctg cttgcgaacg aggtcggcct tgatttcgct gttcttctcg tc
//
LOCUS       DASUGB010000461         3906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_192389, whole genome shotgun sequence.
ACCESSION   DASUGB010000461 DASUGB010000000
VERSION     DASUGB010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192389"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFV87_08860"
     CDS             <1..644
                     /locus_tag="VFV87_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103906.1"
                     /translation="PIQWQFSLSRFEKSLDSECFRVEIRCLSGTDGHPLTTLWVDRQS
                     RALRQIATEIPVPGGYQTMTVSYEFDSGQPGPVLGPLTALPIDLPVLLADGAKGLETF
                     HYRSEIGPPGRKELDALGFAHEVEQRATTASADDVRMLMATVFAKSLDDGVLARSLIE
                     NPVTDIRLKSAGREIRQLWQAGSPWPIYCDNGQTVCRLVSVEPAAQPAEEERE"
     gene            641..2155
                     /locus_tag="VFV87_08865"
     CDS             641..2155
                     /locus_tag="VFV87_08865"
                     /inference="COORDINATES: protein motif:HMM:NF027993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX5103907.1"
                     /translation="MNIVVKAACCLVILLLVAGTARGQVDEGSAKHIPWSGYWFPMAK
                     GEILEPLRKYDALTGRNAFAWEYQRNPPGQNVPEWHGYCHAWAAAAVLEKEPSQVRAA
                     HYGNGSIPLRISDQKAWLTACHSADAANSYGDRYGDGQGMEDPGDLTPDALWQLLRMF
                     LRDQRVALIMDLDPGPEVWNYPVYAYRIEYGPHPAAQGWFHAQLGLWAADDAVPPDFV
                     GLVPHYQTYTFQFQMRGRSIVAGSARWTGQSVQDHPDFAWYPYVVMAENPELHYPNVK
                     KLLQSSTPVGAPASEGNGATGVQPHIPGFSPVDSSGQSDASNQPVPVSPMQLVSAVAN
                     RTSKFGFDLRSAEFGKVQYAIGEGLQLVGTSSQDGYLYLLHIDPLGELTLLYPSPGDD
                     NRVAASVEFRFPRQKVGEVRGPLGNHCVKAIITQQPLILTGFSHMPRRDATAEAWQPR
                     TLLLCPTQTKLVKSLLGEYVQGKSLDDRQLPGDIASRLPGFAQDEFTSYVGPAR"
     gene            2189..3583
                     /locus_tag="VFV87_08870"
     CDS             2189..3583
                     /locus_tag="VFV87_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5103908.1"
                     /translation="MNYLQGVSGTLGLVAVAALALAEEPERNPRGVLIEQVRNDQPAF
                     MVRIDVDHADRVYHPGDELRVRVRSGENGYLYLLYCDAANRVSCLYPNAFHADNRVAA
                     NQDVFLPRPVAAGEEGFRITMGPPFGQELLKAVVFKEQLAPEMLARLMKSVESAAIQP
                     GARGAVVEQVKSLDWAEHHVAIEMKAREAAGQPRTARRVALCVGISAFADPQIRDLAV
                     AHRDAAEIAKSLQQRCGFDQVWGLVNEHATRANIEQAIRRQLPAATRPGDTVLIYWSG
                     HGSRCADDGGDEQDGFDEYLVPYDGKLTNVETIRQSMILDDAFGRWIQDLDGRRVVVI
                     LDTCHSGGQGQGKSLPAAETAAAAPKGFDFLDGELSRIKDIGQRETALIASSAASQVS
                     FERREGDLSTMTYFLLAQLNAEGGSLELKQVFDVLERDVPRYIERAFPGTTQTPLMLS
                     NLSAPLYLRLGPSE"
     gene            3695..>3906
                     /locus_tag="VFV87_08875"
     CDS             3695..>3906
                     /locus_tag="VFV87_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103909.1"
                     /translation="MARFSAILVFGACLAASQVAQAQLQVKWMNANQHNGMNNNAAAA
                     AFWGAVGNAIQNEINKNKGGKPGWGHG"
BASE COUNT          740 a         1194 c         1241 g          731 t
ORIGIN      
        1 agcccattca gtggcaattc tctctttccc gatttgagaa gtcgctcgat agcgagtgct
       61 ttcgcgtgga aattcgctgc ctgtccggca cggacggcca tcccctcaca acgctgtggg
      121 tggaccgcca atcgcgagca ctgcggcaaa tcgccaccga gattcccgtt cccggcggct
      181 atcagaccat gaccgtcagc tacgaattcg attccgggca gccaggaccg gtcctcggtc
      241 cgctcaccgc cttgccgatc gatctgccgg tcctgctggc agacggggcg aaaggattgg
      301 agacctttca ttaccgatcc gagatcggtc cgccgggacg caaggagctc gacgcgctgg
      361 gattcgctca cgaagtcgag cagcgggcta ccacggcaag tgccgacgat gtccgcatgc
      421 tgatggcgac ggtgtttgcg aaatcgcttg acgatggcgt actcgccagg tcgcttatcg
      481 aaaatccggt caccgacatc cgactcaaat cggccggacg cgagatcagg caactctggc
      541 aggccggctc gccctggccg atctactgcg acaacggcca gaccgtctgc cgcctggttt
      601 cggttgagcc cgccgcacag ccggcggaag aggagcggga atgaacatcg tcgtcaaagc
      661 tgcgtgttgc ctcgttatcc tgctgcttgt cgccggcacg gcccgtggtc aggtcgatga
      721 gggatctgcg aagcacattc cctggtcggg atattggttc ccgatggcca agggcgaaat
      781 cctggagccg ctgcgcaagt acgacgccct cacgggccgc aacgcatttg cgtgggaata
      841 tcagcggaac ccgccgggcc agaacgtccc tgagtggcac ggctattgtc acgcctgggc
      901 tgcggcagca gtcctggaga aagagccgag ccaagtgcgg gccgcccact acggcaatgg
      961 ctccattccg ctgcgtatca gcgaccagaa ggcctggttg acggcctgcc attcggccga
     1021 cgcggcgaac tcctacggcg atcggtacgg cgacggccag ggaatggaag atccgggcga
     1081 tctgaccccg gatgccctct ggcaactgct gcgaatgttc cttcgcgatc agcgcgtggc
     1141 gctcatcatg gatctggatc cgggaccgga ggtctggaac tacccagtct atgcctatcg
     1201 cattgaatac gggccgcatc ctgcggcgca aggctggttc cacgcgcagc taggcctgtg
     1261 ggcagccgac gacgcggtcc cgcctgactt cgtgggcctg gtgccgcatt accagaccta
     1321 caccttccaa ttccaaatgc gcggtcgctc gatcgtggcg ggcagcgcgc gctggactgg
     1381 ccaaagtgtg caggaccatc ccgattttgc ctggtatccc tatgtggtca tggccgagaa
     1441 cccggaactg cactacccga acgttaaaaa gctcttgcag agcagcacgc ccgttggggc
     1501 gccggcgagc gagggaaatg gtgcgacagg cgtccaaccg cacatcccgg gcttttcgcc
     1561 cgtcgattct tccggccaaa gcgatgccag caaccagcct gtgccggtca gccccatgca
     1621 actcgtctcc gccgttgcca atcgcacctc gaagttcggc tttgacctgc ggtccgcgga
     1681 gtttggcaag gtgcaatatg ccattggcga agggctgcag cttgtcggaa cctcgtcgca
     1741 ggacggatac ctctatttgc tgcacatcga tccgctgggc gaattgacgc tgctctatcc
     1801 cagcccgggg gacgataacc gcgtggcagc tagcgtcgag tttcgctttc cgcggcaaaa
     1861 ggtgggcgag gtgcgcggcc cgctgggaaa ccactgcgtc aaggcgatca tcacccagca
     1921 gcctttgatt ctcaccgggt tttcgcacat gccgcggcgg gacgccacgg ccgaagcctg
     1981 gcagcctcgc acgctgctcc tctgccccac gcagaccaag ctcgtcaaat cgttgctggg
     2041 cgaatacgtc cagggcaaga gcctggacga ccggcaattg cccggcgaca ttgccagccg
     2101 gctgccgggc ttcgcacaag acgaattcac gtcttacgtt ggccccgcac gttaggaaca
     2161 gaccgcgctt ttcgataagg atgtggccat gaactacctc caaggcgtct ccggcacgct
     2221 cggactggtg gccgttgctg cgctggccct ggcggaggaa ccggaacgca atccgcgcgg
     2281 agtcttgatc gagcaagtcc gcaacgacca gccagccttt atggttcgca tcgatgtgga
     2341 tcatgcggac cgcgtgtatc acccgggcga cgaactgcgg gtccgcgtca gatcgggcga
     2401 gaatggctac ctgtacctgc tgtactgcga tgcggcgaac cgggtttctt gtttgtatcc
     2461 caacgcgttt catgcagaca atcgtgttgc ggccaaccag gatgtgttcc tgccgcgccc
     2521 ggttgctgca ggcgaggagg ggttccgaat cacgatgggt cctcccttcg gccaggagtt
     2581 gctgaaggcc gtggtattca aggagcaact ggcgcccgaa atgctggctc gtttgatgaa
     2641 gtcggtggag agcgctgcca tccaaccggg cgcgagaggc gccgtggtgg agcaggtgaa
     2701 gagcctggac tgggccgagc accacgtggc catcgagatg aaggccaggg aagcggccgg
     2761 acaaccccgc acggcacgtc gagtggccct ttgcgtgggg atcagcgcgt tcgccgaccc
     2821 gcagattcgc gatctggcgg tggcccatcg cgatgcggcg gaaatcgcca aatcattaca
     2881 acagcgctgc ggattcgacc aggtctgggg tctggtgaac gagcacgcga cacgggccaa
     2941 tatcgagcag gcgatccgcc ggcagcttcc cgcggcgacg cgcccgggcg ataccgtttt
     3001 gatctattgg tcgggacatg gctcgcggtg cgccgatgac gggggtgatg agcaggacgg
     3061 gtttgacgag tatctggtcc cgtacgacgg caagctaacg aacgtagaaa ccatccgcca
     3121 atcgatgatt ctggacgatg cgttcggccg ctggattcaa gacctcgacg gccggcgggt
     3181 cgtggtcatt ctcgatacct gccacagcgg cggccaaggg caagggaagt cgctgccggc
     3241 ggctgaaacg gcagcggcgg cgcccaaggg gtttgatttc ctggatggcg agctctcccg
     3301 gatcaaagac atcggccagc gtgagacggc cctgattgca tcctcggctg cgagccaggt
     3361 ctcgttcgag cggcgtgagg gggacctcag cacgatgacc tactttttgc tcgcccaact
     3421 gaatgcggaa ggcggatcgc tcgagctgaa gcaggtgttc gatgtcctcg aacgcgacgt
     3481 gccgcgctac atcgagcggg cctttcccgg tacgacccaa acgcccctga tgctcagcaa
     3541 tctgtccgcg ccgctctacc tgcggctggg tccgagcgag tgattctctg cggccatttt
     3601 tcaggaatct tggccgcttc gcatgtcggt ttggaagcgg atttggccat ttcagcggtg
     3661 tatctctgcc tcacgttcca agaggaggat agccatggca cgcttttcgg cgattctggt
     3721 tttcggcgct tgcctggcag cttctcaggt ggcccaggca cagctccaag tgaagtggat
     3781 gaatgccaac cagcacaatg gcatgaacaa caacgccgca gcagccgcgt tctggggcgc
     3841 cgttggaaac gccattcaga atgagatcaa taagaacaag ggaggcaaac ccggatgggg
     3901 tcatgg
//
LOCUS       DASUGB010000462         8626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_470927, whole genome shotgun sequence.
ACCESSION   DASUGB010000462 DASUGB010000000
VERSION     DASUGB010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470927"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFV87_08880"
     CDS             <1..992
                     /locus_tag="VFV87_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103910.1"
                     /translation="DPAITFLQTGSQQPGRPSFGAWLSYGLGSENDNLPAFVVMISQG
                     SGNKTDQPIFSRLWGSGFLPSEHQGVRFRAGVNPILYLSNPAGIDGQSRRDMLDGVAA
                     LNEISSQQLGDPEIETRIAQYEMAFRMQTSVPELTDLSGESQETIDLYGIKDDGVDGG
                     FARNCLLARRMAERGVRFIQLMHRGWDQHSSLPQQIRGQCSDVDRPSAALIKDLKDRG
                     LLDETLVIWGGEFGRTVYSQGALTATNYGRDHHGRCFTLWMAGGGLKPGITYGETDDY
                     CYNVVKDPVHVHDFQATVLHCLGIDHTRLTYRFQGRDFRLTDVHGNVVNGLLV"
     gene            1007..1219
                     /locus_tag="VFV87_08885"
     CDS             1007..1219
                     /locus_tag="VFV87_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5103911.1"
                     /translation="MMLADVFHSDPEILGGTPVFTGTRVPVVNLIDHLEAGDSIDDFL
                     EGFPSVRRDQVIAFLEAARQQLLQSA"
     gene            1221..1541
                     /locus_tag="VFV87_08890"
     CDS             1221..1541
                     /locus_tag="VFV87_08890"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5103912.1"
                     /translation="MQILIDECLDWRLKREFPGHSARTVTDMGWGGIKNGALLALAQA
                     RFEVFITGDRNLSRQQQPANFSLAIIVLRATSTRLADCLPLIPKVIAALPGLQAGTVT
                     EIHP"
     gene            1553..2263
                     /locus_tag="VFV87_08895"
     CDS             1553..2263
                     /locus_tag="VFV87_08895"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5103913.1"
                     /translation="MPELGVNIDHVATIRQARKTYEPDPVAAAVLCELAGADAITIHL
                     REDRRHIQDRDVRVLRETVTIKLNLELSVADEIVGIACQVQPQQATIVPERREEITTE
                     GGLDVVTHRAATAKAIERLREAGISVSLFLDPDPRQIELARQLGADAVELHTGQYALA
                     SGAAQHQQLVALTTAGKLICDLGMHLHAGHGLNYRNVVPVARIPQMRELNIGHSIVSR
                     AVLVGFDRAVREMKALMG"
     gene            2349..3194
                     /locus_tag="VFV87_08900"
     CDS             2349..3194
                     /locus_tag="VFV87_08900"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5103914.1"
                     /translation="MSIPLQEVVCCLGQPVAGNPTQYMMEKAFEAAGLDWRYLTCEVP
                     PERLEDAMRGIRALGFKGANFTIPHKVAVLEYLDELSEAAELMGAVNCVNRVGEKLIG
                     ENTDGKGFVQSLREVCNPAGKRIAIIGAGGAARAIAVELGLSGAGQIVIVNRSQERGE
                     QLVELLRERVKLAADYSRLEGDYAVPEMADILINATSIGLGDPAAQIPVAASSLRPEL
                     IVADVIFNPPETWLIRTAKERGCRAIDGLGMLVNQAVISFKIWTGQTPDAGVMRDALE
                     EFLEL"
     gene            complement(3274..3903)
                     /locus_tag="VFV87_08905"
     CDS             complement(3274..3903)
                     /locus_tag="VFV87_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5103915.1"
                     /translation="MRGSFVITACCLAMLLPAVAGAGKYNPVLKIGEPAPDYSGLEGI
                     DGKQHALKDLAEKQVVVVAFICNTCDYAKDAEERLIALADKFAAEGGKCALVAINPNL
                     IKDDLLPAMQERAKLREFNFLYVHDGEKQETAKAYGITYTPEFVVLDKDGKVVYLGAL
                     DDDPDGKKVTTRYVEDAVAAALAGKQPATAETPARGCAVRQPPRRRRTE"
     gene            4032..4628
                     /locus_tag="VFV87_08910"
     CDS             4032..4628
                     /locus_tag="VFV87_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX5103916.1"
                     /translation="MFSVLITAFAPYDRWQENSSWLALVELIRDLPPGHKVVTRRYPV
                     DFEVARSRLYDDLATDYDLALHLGQAPGIGRVHLEAIGVNVGGHSSQMPDQFQQLVPG
                     GPAAYQTSLPLAEWAALVRASGIPCQVSYHAGTYLCNAVLYLSQHIAREQNLKTQATF
                     IHLPLAPSQVIGERQDWPTLPPATCAAAVRTILQALVL"
     gene            complement(4732..5277)
                     /locus_tag="VFV87_08915"
     CDS             complement(4732..5277)
                     /locus_tag="VFV87_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103917.1"
                     /translation="MSDFLDIVRDPKNKQTFEKLLRLAASRGDDEQVAERLSWGIDPN
                     CESETGRTPLIVNVTSSSPSSGVVQALLKAGADPTHIDHRGLSALDYIRRKLARINLR
                     PRKPPRKSPSLDENGQLILADFELEMFEETRREHPDSAREFINSYMKERLKAARRVFN
                     DPSEVEKIVELLEAAERQKRT"
     gene            complement(5342..5869)
                     /locus_tag="VFV87_08920"
     CDS             complement(5342..5869)
                     /locus_tag="VFV87_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5103918.1"
                     /translation="MATATAPMPAQPISGVTPAERKEVTVMTVWPSVAALGPGRALGR
                     AFAIQWPDVYIFRLGNLLALLSIPIVLPLYFLKVLPFIGTRYVLTNRRIVVLRGITGK
                     EEKFVDLDRFDTVEVVVQPGQEWYHAGDLVFKQGAVETFRLEGVSRPEAFRATCLKSQ
                     MAHAGVKKARERQRT"
     gene            complement(5996..7759)
                     /locus_tag="VFV87_08925"
     CDS             complement(5996..7759)
                     /locus_tag="VFV87_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX5103919.1"
                     /translation="MANNPDVFQRLIGLETEYALFVPASSGPRPANRYGLFRELVAAL
                     RRKIPCAPARNMKEGVFHAAGGAVWFETERPAVGGGLIEGSTPECRSPRQLLAWQRAQ
                     DELLAEAAAEAFGGEIALLKNDRDASGNVYGAQENYEATLASGWRLLAWRAALVLLLP
                     LATLTWLALWLIEALVALYALTATVVYLSGERLLPRPEWLARLLFGCSVKELESDIPT
                     GPAWLEAVLSLVARVIAAPLATALFAVLWLTAFVRIRRQMTPFLISRAIVCGSGWLDD
                     QGRFQLADKAPAMNCLTGFGGLLGDRPIYCLGHFFKTIYADAWLAPQHYLRLFASRQR
                     LQIALGDSNLTDTAEYLRIGTTLLVLDVIEAGEMPALPRVRRPIRSLRHVCADSSLKA
                     RIPLAGGGVCSPLALQRFYLEACRAFLSRRPGAPSEARDVVQRWEAALDGLEDDPQSL
                     VGTLDWPTKQFVLAKSGRGASWEARKKIDLRYHELSPEGYFQRLKATGLIAELLEERE
                     IDHARRNPPAGTPAAVRGRYIREFAAEEGEIAANWHAVYLGGSGDRTKVIRLDRYLQR
                     PDRPSTLARRKSPKRKDSGGS"
     gene            complement(7749..7925)
                     /locus_tag="VFV87_08930"
     CDS             complement(7749..7925)
                     /locus_tag="VFV87_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103920.1"
                     /translation="MNELFAISPEAPTVRPAEKPAAQPTDPAVLRALAEVERDSQDRP
                     ETYLRDTVVPHGGE"
     gene            8128..>8626
                     /locus_tag="VFV87_08935"
     CDS             8128..>8626
                     /locus_tag="VFV87_08935"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX5103921.1"
                     /translation="MPLMMLLPPNDTIAVLASGGLDSAILIGKLLSGGRRVQPIHIRT
                     GLHWQAGELAALKRFLEAVASDKLGDLVVLDLPLADLYDGHWSLTGRDVPDAETTDEA
                     VFLPGRNALLIVKAAVWCQLRSIGELALATLGTSPFADAGESFFRDFERAMNCGSQRP
                     LRLARP"
BASE COUNT         1529 a         2774 c         2770 g         1553 t
ORIGIN      
        1 acgacccggc catcaccttc ctgcaaacgg gctcgcagca gccggggcgg ccgagcttcg
       61 gcgcctggct cagctatggc ctggggagcg aaaacgacaa cctgccggct ttcgtcgtga
      121 tgatttcgca aggcagcggc aataagaccg accagccgat cttctcgcgc ctctggggga
      181 gcggcttttt gccgtcggag caccaggggg tgcggttccg ggccggggtc aatcccattc
      241 tgtacctttc gaacccggct ggcatcgacg ggcagtcgcg gcgcgacatg ctcgacggcg
      301 tcgcggccct caacgagatc tccagccagc agcttggcga cccggagatc gagacccgca
      361 tcgcccagta cgagatggcc ttccgcatgc agaccagcgt gccggagctg accgacctct
      421 cgggcgaatc gcaggaaacg atcgacctgt acggcatcaa agatgacggc gtcgatggcg
      481 gctttgcgcg gaattgcctc ttggcccggc ggatggccga gcggggcgtg cggttcatcc
      541 agctcatgca ccgcgggtgg gaccagcatt ccagcctgcc gcagcagatt cgcggccagt
      601 gcagcgacgt cgatcggccc agcgccgcac tgatcaagga tctgaaggac cgcgggctct
      661 tggacgaaac cctcgtcatc tgggggggcg agttcggccg cacggtctat agccaggggg
      721 ccctgaccgc gacaaactac ggccgcgacc accacggccg ctgcttcacg ctctggatgg
      781 ccggcggcgg cctcaagccg gggattacgt acggcgagac cgacgactat tgctacaacg
      841 tcgtgaaaga tccggtccac gtccacgatt tccaggccac cgtgctgcac tgcctgggga
      901 tcgaccatac ccggctcacc taccgcttcc agggccgcga ctttcggctg acggacgtgc
      961 atgggaatgt ggtaaacggg ctactggtat gaggaggaat cagaacatga tgctggctga
     1021 tgtttttcat tccgatcccg agattcttgg tggcacgcca gtatttacgg gcacacgcgt
     1081 cccggtggtg aatctgattg accacttgga ggcgggcgac tcgattgatg atttcctgga
     1141 gggctttccg agcgtgcgcc gcgatcaggt gattgctttc ctcgaagctg cccgacagca
     1201 gctcttgcag tcggcctgat atgcaaatcc tgattgacga gtgcctggat tggcgactga
     1261 agcgagaatt ccctggacat tctgctcgga cggtcaccga catgggatgg ggcggcatca
     1321 aaaacggggc cctgttggca cttgcccagg cgagattcga agttttcatc accggcgata
     1381 gaaacctgtc tcgccagcag cagcctgcga acttctccct tgcgatcatt gtcctgcggg
     1441 ccacatcgac tcgcctggcc gattgtctgc cgctgattcc gaaagtcatc gccgctttgc
     1501 caggtttgca ggcaggaact gttaccgaaa tccatcctta gaatctgcac ccatgcccga
     1561 actcggcgtc aacatcgacc acgtggcgac cattcgccag gcccgcaaga cgtatgagcc
     1621 cgacccggtg gcggcggcgg tgctgtgtga gctggcagga gcggatgcaa tcaccattca
     1681 cctgcgggag gaccggcgac acattcagga ccgcgacgtc cgtgtgctcc gcgagacggt
     1741 gacgatcaag ctgaacctgg agctgtcggt cgccgacgag atcgtcggca tcgcgtgcca
     1801 ggtccagccg cagcaagcga cgatcgtgcc cgagcggcgc gaggaaatca cgaccgaagg
     1861 gggcttggac gtcgtcacgc accgcgcggc caccgccaaa gccatcgagc ggctgcggga
     1921 agcgggcatc tcggtcagcc tgttcctcga ccccgatcca cggcagatcg agctggccag
     1981 gcaactcggc gccgatgcgg tcgagctgca cacgggccaa tacgccctgg ccagcggcgc
     2041 ggcccagcac cagcagcttg tcgctctgac caccgccggc aagctgatct gcgacctggg
     2101 catgcacctg cacgccgggc acggcctcaa ctatcgcaac gtcgtcccgg tcgcccgcat
     2161 tccgcagatg cgcgagctga acatcggcca cagcatcgta tcgcgggccg tcctcgtcgg
     2221 cttcgatcgg gccgtgcggg agatgaaagc cctgatgggc tagtccaaag tccgacgtcc
     2281 aaagtccaag gtcggatccg ccaacgttgg accttggact ttggactttg gaccagtatc
     2341 ttcacggcat gtccattccc ttgcaggaag tcgtctgttg cctcggccag ccggtggccg
     2401 gcaatcccac gcagtacatg atggagaagg ccttcgaggc cgcggggctc gactggcggt
     2461 atctcacgtg cgaagtgccc cccgagcgac tggaagatgc gatgcgggga attcgagccc
     2521 tgggcttcaa gggggcgaat ttcacaatcc cgcacaaggt ggcggtgctc gaatacctgg
     2581 acgagctgag cgaagcggct gagctgatgg gagccgtcaa ctgtgtcaac cgcgtcggcg
     2641 agaagctgat cggcgagaac accgacggca aaggcttcgt ccagtcgctc cgtgaggtct
     2701 gcaacccggc cggcaagcgg attgcgatta tcggcgccgg gggcgcggcg cgggcgatcg
     2761 ccgtggagct gggcctgagc ggtgcggggc agatcgtgat cgtcaaccgc tcgcaggagc
     2821 gcggcgagca gctagtcgag ctgctgcggg agcgagtcaa gctggccgcg gactattcgc
     2881 ggctggaggg cgattatgcg gtgcccgaga tggccgacat tctgatcaat gccacgtcga
     2941 tcggcctggg tgatccggca gcccaaattc ccgtcgccgc cagctcgctg cggccggagc
     3001 tgatcgtcgc cgacgtgatc ttcaatccgc ccgaaacgtg gctcatccgc acggcgaaag
     3061 agcgcggctg ccgggcgatc gacggcctgg gaatgctcgt caaccaggcc gtgatcagct
     3121 tcaagatctg gaccggccag acacccgatg ccggcgtgat gcgcgatgca ctggaggagt
     3181 tcttggagct gtaggacggg tttctaaccc gttcgccttg ttgccagcgg tttgtaggcg
     3241 acggacgggt tggaaacccg tcctacgtgg cgatcattcg gtccgccgcc tccgtggcgg
     3301 ctggcggacg gcgcagccgc gggcgggggt ctcggcagtt gcgggctgct tgccggcgag
     3361 ggcggcggct acggcgtctt cgacgtatcg cgtggtgacc tttttcccat cgggatcgtc
     3421 gtcgagcgcc ccgagataga ccaccttacc gtccttatcg aggacgacaa attccggcgt
     3481 ataggtgatg ccgtaagcct tagccgtctc ttgcttttcg ccgtcgtgca cgtacaggaa
     3541 gttgaactcc cgcagtttgg cccgctcctg catggccggc agcaggtcgt ccttgatcag
     3601 gttgggattg atcgcgacca aagcgcactt gcccccttcc gcggcaaatt tgtcggccag
     3661 cgcgatcagc cgctcttcgg cgtccttggc atagtcgcac gtgttgcaaa tgaacgcgac
     3721 gacgacgacc tgtttctccg ccagatcctt cagtgcatgc tgcttgccgt cgattccttc
     3781 cagcccgcta tagtccggcg ccggctcacc aatcttcagc accgggttat acttgccggc
     3841 cccagcgacc gctggaagca gcattgccag gcagcaggcc gtaatcacaa acgatcctcg
     3901 catcacacat ctccttagca acgcatcaca ggaaatctca gcacgcagca tagctcacca
     3961 cccgtgcgtg ccgtactatt ctaacactcg cctgctgtcc tctgtcccct gactcctgtc
     4021 ccctgccccc tttgttctcc gtcctcatca ccgctttcgc gccctacgac cgctggcagg
     4081 agaactccag ttggctcgcg ctggtcgaat tgatccgcga cctgccgccg gggcacaagg
     4141 tggtcacgcg gcggtatccc gtcgatttcg aggtcgcccg ctcgcgactc tacgacgacc
     4201 tggcgacgga ttacgacctg gcgctgcacc tgggacaggc gccgggaatc ggccgcgtgc
     4261 atctggaagc gatcggggtg aacgtcggcg ggcactcctc gcaaatgccc gatcagttcc
     4321 agcagctcgt gcccggcggc ccggctgcct atcagacttc gctgccgctg gccgagtggg
     4381 cggcactggt ccgggcttcc ggcattccct gccaggtctc gtatcacgcc ggcacgtacc
     4441 tttgcaacgc cgtgctgtac ctaagccaac acatcgcccg cgagcagaac ctgaagacgc
     4501 aagccacttt cattcacctg ccgctggcgc cgtcgcaggt gataggtgaa cgccaggatt
     4561 ggccgaccct tcccccggca acctgtgccg ccgcggtccg aacgatcttg caggcgctgg
     4621 tactatgaca gcaagcggca gcagagagtg gtggttatag atgaagggta aaaaatgctt
     4681 ccaatctttt acccccaatt tataaccgcc aaagcttcgt aacggcgcat gtcacgtgcg
     4741 tttctgccgc tccgctgctt ccagcagttc cacgatcttc tcgacctcgc tcggatcgtt
     4801 gaacacgcgg cgggccgcct taagccgctc tttcatatag ctgttgatga actcgcgggc
     4861 agaatcgggg tgctcgcggc gcgtctcctc gaacatttcc agctcaaaat cggccaggat
     4921 gagctggccg ttttcatcca gcgaagggct tttccgcggc ggcttgcgcg gccgcaggtt
     4981 gatccgggcc agcttgcggc ggatgtagtc gagggccgag agcccgcggt gatcgatgtg
     5041 cgtcggatcg gcccccgctt tgagcagcgc ctggaccacg cccgacgacg ggctgctgct
     5101 agtcacgttg acgatcaagg gcgtgcggcc cgtctccgat tcgcagttcg ggtcgatccc
     5161 ccacgacagc cgttcggcca cctgctcgtc atccccccgg ctggccgcca ggcgcagcag
     5221 cttctcgaac gtttgcttgt ttttgggatc gcgaacaatg tcgaggaaat cggacatggt
     5281 aggggagtga gtggtgagta agtgagtggt gtgccgcgtc tgacctctga cctcctgtcc
     5341 tctacgtccg ctgccgctcc ctggccttct tcacgccggc atgggccatc tgcgacttca
     5401 ggcacgtcgc ccggaacgcc tccggccgcg agaccccttc cagccggaag gtttcgaccg
     5461 cgccttgctt gaagaccagg tcgccggcgt gataccactc ctgccccggc tggacgacga
     5521 cctcgaccgt gtcgaagcgg tccagatcga cgaatttctc ctctttgccc gtgatgccgc
     5581 ggagcaccac gatcctgcgg ttcgtgagga cataccgcgt gccgatgaac ggcaggacct
     5641 tgaggaaata gaggggcagc acgatgggaa tggagagcag cgccagcagg tttccgaggc
     5701 ggaagatata gacatcgggc cattggatgg caaaggcgcg tcccagcgcc cggcccggcc
     5761 ccagcgcggc caccgacggc cagaccgtca tcacggtgac ctctttcctc tcagcgggcg
     5821 tcacccccga tatcggctgg gcaggcattg gagcagtcgc agtggccatg agtggttcaa
     5881 aatcccagtt caagaatcaa agctaaaagg ttagcaaagg cccaccggcc catccagcgg
     5941 gagtccgcgc ctggcaccaa taccgagcga gccgcatttg catttacgat gagcttcagc
     6001 tgccgccgga gtctttccgc ttgggagatt tgcggcgggc gagcgtgctg ggcctgtcgg
     6061 ggcgctggag atagcggtcc aggcggatca ctttggtccg gtcgccgctg ccgccgagat
     6121 agacggcgtg ccagttggcg gcgatttcgc cctcctcggc agcgaactcg cggatatagc
     6181 ggccgcgcac ggcggcgggc gtgccggcgg gtggattgcg gcgggcgtgg tcgatctcgc
     6241 gttcttcgag caattcggcg atcaggccgg tagccttgag gcgctggaag tagccttccg
     6301 gcgacagctc atgatagcgg aggtcgatct tcttccgggc ttcccacgaa gcgccgcggc
     6361 cggacttcgc gaggacgaac tgcttcgtcg gccagtcgag cgtgcctacc agcgattgcg
     6421 ggtcgtcctc caggccgtcg agtgccgcct cccaccgctg gaccacgtcg cgggcctcgc
     6481 tcggcgcgcc aggccggcgg ctgaggaacg cccggcaggc ttccaggtag aaccgctgca
     6541 gcgccagggg cgagcaaacg ccgccgccgg ctagcggaat gcgcgccttc agcgagctgt
     6601 cggcacagac gtgccgcagg ctgcggatcg gccggcggac ccgcggcagc gcgggcattt
     6661 cgccggcttc gatcacgtcc agcaccagca gcgttgtgcc gatccgcaga tactctgccg
     6721 tgtcggtcag gttcgaatcg cccaaggcga tttgcagcct ttgccggctg gcgaacagcc
     6781 gcaggtaatg ctgcggagcg agccaggcat cggcatagat cgtcttgaaa aaatgcccga
     6841 ggcagtagat cggccggtcg cccaaaagtc cgccaaagcc ggtcaggcag ttcatcgccg
     6901 gggccttgtc ggccagttgg aagcggcctt ggtcgtccag ccagccgctg ccgcagacga
     6961 tcgcccggct gatcaggaaa ggggtcatct gccggcggat gcggacgaaa gccgtcagcc
     7021 acaaaacggc gaacagcgcg gtcgccagcg gcgcggcgat cacgcgggcg acgagcgaca
     7081 acaccgcctc cagccacgcc ggacccgtgg ggatatcgct ttcgagctct ttcaccgagc
     7141 agccgaagag caaccgggcg agccactcgg gccgcggcag caggcgttcg cccgaaagat
     7201 agacgacagt ggctgtcagg gcatacaggg cgacgagcgc ttcgatcagc cacagggcca
     7261 gccacgtcag ggtggccagc ggcaagagca gcaccagcgc cgcccgccac gcgagcagtc
     7321 gccatccgct ggcgagcgtc gcttcgtagt tttcctgggc gccatacacg ttgccggagg
     7381 catcgcgatc gttcttcaag agggcgatct cgcccccaaa ggcttccgcg gctgcttcgg
     7441 ccaagagttc gtcctgcgcc cgctgccagg cgagaagctg ccgcggcgag cggcactcgg
     7501 gtgtggagcc ttcgatcagc ccgccgccca ccgcggggcg ttcggtctca aaccacacgg
     7561 cgccgcctgc cgcatggaag acgccttctt tcatgttcct ggcgggagcg cagggaatct
     7621 tcctccgcag cgccgccacc agctcgcgaa acagaccgta gcggttggcc ggccgcggcc
     7681 cggaagacgc gggcacaaag agagcatatt ccgtttccag cccgatcagg cgctggaaaa
     7741 cgtccgggtt attcgccacc gtggggcact accgtatctc gcaggtaggt ttcgggacgg
     7801 tcctggctgt cgcgctcgac ttccgcgagg gcgcgaagaa cggcaggatc ggtcggctgc
     7861 gcggcgggtt tctcggccgg gcgaacggtg ggcgcttcag gcgaaatcgc gaacagctca
     7921 ttcatgactg gggcatcccg gaggcttgcg aggaggatca ttccgtaccg gcgccagccg
     7981 gcaaggttcc ttcatcctag tcccgccctg cctctgcagc aagcgcgcgg caaaggggag
     8041 cagtggtcat tccttaacgg ccggtttacc atcgatggca gcgactcgac gctccctccc
     8101 agaacgcgcc gtcaaccagc gactagaatg cctttgatga tgctcttgcc gcccaacgac
     8161 acgattgccg ttctcgccag cggcgggctg gatagcgcga tcttgatcgg caagctgctg
     8221 agcggaggac ggcgcgtcca gccaatccac attcgcacgg gcttgcactg gcaagcggga
     8281 gaactggcgg cgctaaagcg gttcctggag gcggttgcca gcgacaagtt gggagacctc
     8341 gtcgtgctcg atttgccgct ggcggatctg tatgacgggc attggagcct gacgggtcga
     8401 gatgttccag acgccgaaac aacggacgag gcggttttcc ttcccggtcg aaacgccttg
     8461 ctcatcgtca aagctgccgt gtggtgccag cttcgcagca tcggcgagtt ggcgttggcg
     8521 acattgggaa ccagcccgtt tgccgacgcc ggcgagtcgt tcttccgcga cttcgagagg
     8581 gcgatgaatt gcgggagtca gcgccccttg cgcctcgccc gcccgt
//
LOCUS       DASUGB010000463         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1165023, whole genome shotgun sequence.
ACCESSION   DASUGB010000463 DASUGB010000000
VERSION     DASUGB010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165023"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..895)
                     /locus_tag="VFV87_08940"
     CDS             complement(218..895)
                     /locus_tag="VFV87_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103922.1"
                     /translation="MFRSLLIFAIAFLSIALVMPGVTLSQVPPPAYSYGYGYGGWPTP
                     YLRHSSTYEEGVQRGYADVVRSWGMSNLLNAEAAKAYEQARRDYLDNRLKATQTYFEM
                     RRYNQDYRNANRPRPLTTEEYVRLARLQAPDRLSVSQLDPFTGQINWPVSLRRPEYDE
                     YRQRIERLFRSRAGGTVFVYDEIDEACDAFVDRLQLDIEQFEQNDYIRAKNFVQSLAY
                     EAQLVQR"
     gene            1050..>2534
                     /locus_tag="VFV87_08945"
     CDS             1050..>2534
                     /locus_tag="VFV87_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5103923.1"
                     /translation="MRCTFALLLILGVALTTQGAPPPDLEKWTAGLASSEPQVQYAAA
                     DSLADLGIYAQPAIPHLVKALQGKDADLRWRAARALGAIGSEQAVVALNQAAKDAHAG
                     VRAQVLFALGRIGAADAATTETIAAGLADPDAGVRRASVRALRMIQTDRKLVLPKVLK
                     LLEDADPSVVMPALHTVAEAGKDVVPALKDSLQHPEARYWACLVLSEIGPEAHEAVPA
                     LTELLADKRPEVRLQATIALGEIGPAAKSAAPALGKLLQDEFPAVRAAAVFALGKIGN
                     SAAAEAIARADQADDPFLHTLSVWAQAKLSPDDAARSKEAVTLLVSELGGEDRELAHL
                     AARALADLGATNETVEAELDKVLMGADAATADRTITALASLGPRVVPHAIRGLAVLER
                     RPAALRVLKELGPEAAPAVPNLMEILKGERDPQAQAEVLFVLAAVGPQAEPAVPAVAT
                     ALGSADRDVVLTAAYCLGQIGPEAKAAVPELKKLLAAEDKLVQLT"
BASE COUNT          455 a          755 c          884 g          440 t
ORIGIN      
        1 gcctctcgag acgcgacgcg gcgacgagcg cgggcaaatc aaatccgcgc tgcgacatcc
       61 attgtgcgac ggagaacatc tgcggcatac cggcgcggcc ttgcgtttcc tgcgggcacc
      121 aacttgcagt ctattttgcg cgccgcgcgg ggtcacgcgg tagggagggc gaggaaaagc
      181 cattgccccg cgatttgccg tctcggctga aactgtgcta ccgctggacg agctgcgctt
      241 cgtaggccag gctttgcacg aagttcttgg cccggatgta gtcgttctgt tcgaactgct
      301 cgatatcgag ctgaagccga tcgacgaacg cgtcgcacgc ctcgtcgatt tcgtcataca
      361 cgaacaccgt tccaccggca cgcgagcgga agaggcgttc gatccgctgg cggtactcgt
      421 cgtactcagg tcggcggagc gagacgggcc aattgatctg accggtaaat ggatcgagtt
      481 ggctgacgct caagcgatcg ggagcttgca accgggcgag ccgcacgtat tcctcggtgg
      541 tgagcggccg cgggcggttg gcgttgcgat agtcctggtt gtagcggcgc atttcaaaat
      601 aggtctgcgt cgccttcagc cggttgtcga gataatcgcg gcgggcctgt tcgtaggcct
      661 tggccgcctc cgcgttgagc aggttggaca tcccccacga gcggacgacg tcagcgtagc
      721 cgcgctgcac gccttcctca tacgtggagg agtggcgcag gtatggagtc ggccagcctc
      781 cgtacccgta gccataggaa taagctgggg gcggcacctg cgacaaagtg acgcctggca
      841 tcacgagtgc aatcgacaga aaggctatgg cgaatattag cagcgagcga aacatgctga
      901 gtctcctcgg tctcctcgtg tcatatcggg aatacgcaag cggcaagtag caaatccggg
      961 gccgatgggc gcaaaaattg caagtcccgg cgtacagtcg gcgtgagtct gaagacgctc
     1021 ctgtccattg ctacgctaag aggtcttgta tgcgctgcac ctttgctctg cttctaatcc
     1081 tcggggttgc actcacgacg cagggcgctc cgccgccaga cctggagaag tggaccgccg
     1141 ggctggcgtc ttccgagcct caggtgcaat acgcggctgc cgattcgctg gccgatctgg
     1201 gaatctatgc gcagccggcg attcctcacc tggtaaaagc gctgcagggg aaggatgcgg
     1261 acctacggtg gcgggcagcc cgcgccctgg gagcgattgg cagcgagcaa gcggtggtgg
     1321 cgcttaacca ggcagccaag gatgcacacg caggggtgcg ggcgcaggtg ctgttcgcac
     1381 ttggtcggat cggggcggca gacgcggcga cgacggaaac gattgcagcc gggctggccg
     1441 atcctgacgc gggcgtgcgg cgggcttcgg tccgcgccct gcggatgatc cagacggacc
     1501 gcaagctcgt cctgccgaag gtgctcaagc tgctggaaga cgccgacccg tccgtggtaa
     1561 tgccggccct gcacacggtg gccgaagcgg ggaaggacgt tgttcctgcg ctgaaggatt
     1621 cgctacaaca cccagaagcc cggtactggg cgtgtctcgt tttgtcggag atcggccccg
     1681 aagcccacga ggcggtgccg gcattgaccg agctgctggc cgataaacgt ccggaagtgc
     1741 gcctgcaagc gacgattgcc ctgggcgaga tcgggccggc ggccaaatcg gctgcgcccg
     1801 cgctgggcaa gctgctgcag gacgaattcc cagccgtgcg cgcagcggcg gtctttgcct
     1861 tgggcaagat cggcaattcg gccgctgcgg aggcgatcgc cagagcggac caggcggacg
     1921 atccgttcct gcacacgctg agcgtctggg cacaggcaaa gctgagcccg gacgacgcgg
     1981 cccggtcaaa agaagcggta actctgcttg tgagtgagct gggcggcgag gatcgcgagc
     2041 tggctcacct ggctgcgaga gccttggccg acctgggagc gaccaacgag acggtcgaag
     2101 cggagctgga caaggtcttg atgggcgccg atgccgcgac tgccgaccgg acgatcacgg
     2161 ctttggcgtc gctggggccg cgcgtcgtgc cgcacgccat tcgcggactg gcggtgctgg
     2221 agcgccgacc cgcggcactg cgtgttctca aggagctggg gcctgaagcc gcccccgccg
     2281 tcccaaatct gatggaaatc ttgaagggtg agcgggatcc ccaggcgcag gcggaagtat
     2341 tgttcgtgct ggccgctgtc ggtccgcagg cggagccggc ggttcccgcc gtggcgactg
     2401 cgctcggatc cgcagatcgc gacgtcgtgc tgaccgcggc ttattgcctg gggcaaatcg
     2461 gaccagaggc caaagccgcc gtaccggagc tgaagaagct gctcgctgcg gaggataagc
     2521 tcgtgcagct tacg
//
LOCUS       DASUGB010000464         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_471056, whole genome shotgun sequence.
ACCESSION   DASUGB010000464 DASUGB010000000
VERSION     DASUGB010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471056"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VFV87_08950"
     CDS             complement(<1..1424)
                     /locus_tag="VFV87_08950"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5103924.1"
                     /translation="MQRARRSEEASEFAAAEATLREALRQARSFGDKTHPTLLEVVHR
                     LSALHYYMGEYESAASEMTEAVEISRALYGEQDEEYLASVGNLAALEHARGHFSDALR
                     HSLAVAKATKERYGDSHPSYATALNNLAGVYQSLGDFQRSITNLQQVVNIRSQLTGED
                     YADLLTAQNNLAGVHLLVGEPAKAQGLLATVAAGRRKLLGPADPAYAASLNNLAAAEE
                     ALGKLGDAERDYREALRIREQTLGPKHPDVAGSLTNLAALLRKRGCVEQAEELLQRAI
                     QIREAALGRWHPDYAVSLNNLAVHYRQCGRYQEAAEWYRRSLAVQHQIIEATLPALAE
                     QQQITMLQEWRRTLDGFLSVAPVAGTPVEDVYRHVLWWKGRVQTRQLQRRHATDEHTA
                     SRLARLRAISGHLAALTLKPDASREAANQRLLQDLVEERERLESELGLSADRVKTRQA
                     VSPHELAAALPDDAVLVDFLEYYCT"
     gene            complement(1580..>2446)
                     /locus_tag="VFV87_08955"
     CDS             complement(1580..>2446)
                     /locus_tag="VFV87_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX5103925.1"
                     /translation="GLGAVFYELLVGQPPRHAVAMPEMLECVQSGEVDLAPLANPAIP
                     QPLAAICRQALSPQPEDRQRSVELLGEQIERWQKSRSSRSSRLVIGIAAAAVGLLLAV
                     GAALLANSSPDRSDAGASAIAANLVGHAPHRDFALSAEVLGHPAKDGIVALDAGDKVA
                     VRLQSDLECYVGVWHIDAATKEITLLFPNDLEQDHFLAAGDSLTIPGRDSYAIEVSTG
                     AGLEYLWIAASSQRWDAAAGREQGPGVVLEQSGAGGSSEQRAATVVARSGPRISEDVF
                     RLDVKPLSESAR"
BASE COUNT          461 a          807 c          725 g          453 t
ORIGIN      
        1 gtgcagtagt actccaaaaa atctaccagc accgcatcgt ctggaagcgc cgcggccaat
       61 tcgtgcggcg aaacggcctg ccgcgtcttg actcgatcgg cgctcaatcc tagttccgat
      121 tccaaccgtt cacgctcttc gaccaggtct tgaagcaacc gttgattcgc ggcctcgcga
      181 ctcgcgtcag gcttgagcgt tagcgccgcc agatgtcctg aaatcgcccg cagacgcgcc
      241 agccgcgagg cggtatgctc gtcagtcgcg tgccggcgct gaagctgcct ggtttgcaca
      301 cggcccttcc accacagcac gtggcgatac acgtcttcca caggcgtgcc agccactggt
      361 gcgaccgaca aaaatccatc gagcgtgcga cgccactcct ggagcatggt gatctgttgc
      421 tgttcggcca aggccggcag ggtcgcttca atgatctggt gctgaaccgc cagggaccgg
      481 cgataccact cggccgcctc ttggtaacgc ccgcattggc ggtagtgaac ggcgagattg
      541 ttcaggctca cggcataatc cggatgccag cggcccagtg cagcctctcg gatttggatt
      601 gcccgctgca gcagttcctc ggcctgctca acgcagccgc gcttgcgcag cagcgccgcc
      661 agattcgtga ggctgcccgc cacatcggga tgcttcggcc ccagggtctg ctcgcgaatg
      721 cggagggctt cgcgataatc ccgttcggcg tcgccaagct tgccgagcgc ttcctcggcc
      781 gcggccagat tattcaggct cgcggcatag gcgggatcgg ccggacccag caacttccgt
      841 ctcccggcgg caaccgtcgc gagtaagcct tgcgctttcg ccggctcgcc aaccagcaaa
      901 tgcacgccag ccaggttgtt ttgcgccgtc agcaagtccg cataatcctc gccggtcagc
      961 tgactgcgga tattcaccac ttgctgaagg ttcgtaatag accgctgaaa atcgccgagc
     1021 gactgataga cgccggccaa attgttgaga gccgtagcgt agcttggatg cgaatcacca
     1081 taccgctcct tggtcgcctt cgcgacggcg agtgaatgcc ggagcgcgtc cgaaaaatgg
     1141 cctcgggcat gctcgagcgc agccagattg ccgacgctgg cgaggtattc ttcatcttgt
     1201 tccccgtaga gcgcacgaga gatctccacg gcctccgtca tttccgaagc ggccgattcg
     1261 tactctccca tgtagtaatg cagagcgctt agccggtgta caacttccag caaagttgga
     1321 tgcgttttgt cgccgaacga tcgcgcctgc cgcagcgcct cgcgcagcgt ggcttcggcg
     1381 gcagcgaatt cggaggcttc ttcgctgcgc cgtgcacgct gcaaaaggtc ttcgatcggt
     1441 ccgaccggat tgctgtgttc ctgggcaaga gcgccgcaaa cacaacccag gcagaccgcc
     1501 agcgatccga attgccagga tcttcggaga atgtggagtg tagcgatatc catgagcgct
     1561 caacgcctct gcaaatgggt tagcgcgcgg attctgatag tggtttgacg tctagccgaa
     1621 acacgtcttc gctgatgcgc gggcccgaac gggcaaccac cgtggccgct ctctgttcgg
     1681 agcttcctcc tgcacccgat tgctccagga cgacgccggg gccttgctcg cggccggcag
     1741 ctgcatccca cctctgggaa gatgctgcaa tccacaggta ctcgaggccg gcgcccgtgc
     1801 tcacctcgat cgcgtagcta tcgcggccgg gaatcgtcag ggaatcaccg gcagccaaaa
     1861 agtgatcctg ctccaggtcg ttgggaaaga gcagcgtgat ctccttggtc gcggcgtcga
     1921 tgtgccacac tcccacataa cactccagat cgctctgcaa tcgaacggcg accttgtcgc
     1981 cggcatccag ggcgacgatc ccgtctttcg cgggatgacc caacacctcg gccgacaggg
     2041 caaaatcgcg gtgcggggca tgccccacca gattggcggc aatcgcgctg gcccctgcgt
     2101 ccgaccgatc cggcgaggag ttggccaaca aggccgcgcc aacggcaagc aaaagtccca
     2161 ccgcggcggc ggcgattccg atcaccagcc gcgaactgcg cgacgatcga gacttttgcc
     2221 aacgctcgat ttgttctccc agcagctcaa ccgaccgctg ccgatcctcg ggctgcggcg
     2281 aaagtgcctg gcggcaaatc gcggccagcg gctgcggaat cgcgggatta gccagaggag
     2341 caaggtcgac ctcaccgctt tgcacgcact ccagcatttc gggcatggct acagcgtgtc
     2401 gcggcggctg acccaccagc agctcgtaaa acacagcccc cagccc
//
LOCUS       DASUGB010000465         9237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1999153, whole genome shotgun sequence.
ACCESSION   DASUGB010000465 DASUGB010000000
VERSION     DASUGB010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999153"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFV87_08960"
     CDS             complement(<1..317)
                     /locus_tag="VFV87_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5103926.1"
                     /translation="MSDLPRILVVEDEPEIRRFLRASLAANQMQVAEAESGQAGIRAA
                     ASQPPDVVLLDLGLPDVSGFEVIAHLRGWSRVPIIVLSARGQEADKVAALDAGADDYL
                     TKP"
     gene            complement(348..3083)
                     /locus_tag="VFV87_08965"
     CDS             complement(348..3083)
                     /locus_tag="VFV87_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX5103927.1"
                     /translation="MADPDRPNPDQLLARVQAEEAKARRGKLKIFFGAAPGVGKTYAM
                     LEAARKVAKEGVNVVVGYIEPHARPETQALVLGLDVLPKRTVNYRGRQLLELDLQAGL
                     AAKPQLIVVDELAHTNAIDDEQPLVHAKRWQDVEALLAAGIDVYTTVNVQHLESLNDV
                     VAKITGVIVRETLPDSVFEQAEEVELVDLPPDDLIGRLREGKVYVPGQAARALEHFFN
                     KGNLIALRELALRKMAERVDRQMTDYRTDQAIEKTWPAAERLLVCVTPGPTSPSLVRA
                     TRRMAASLRAPWIAVHVETPTDARLRPSDRERLAETLQLARRLGGEVVTLGGADSVGE
                     IVAYARQHNVTKIIVGKTLHSRWRELIRGSFIYDLARRCGDIDVYVISGESTAATRHP
                     AGDERPVRLAGYGAALVAIVVATGLCWAIFPFVSTVTLCMIYLAGVVAVATWHGRGPS
                     ILASFLGVLAFNFFFIHPYYTFAVSDTQYIFTFVVMLLTGLLISTLTGRLTFQASLAR
                     RRELEAAELSELSRALVQIDRLPHLERVRERLQRLVCGQVFPLVAVKAASKTQSRLEP
                     IRVSPGELTPHEQAVASWVADHACPAGLGTDTLPSVGLLFVPLVAAGEIVGVLGVRPA
                     EEGRRFSPDQLRLIETVAGQLGLVVERIQSAELARENQLRYEREKLRSALLSAVSHDL
                     RTPLAAIAGAGSTLAEAADDLHPDARRELAESIVAETDRLNRLIANLLDMTRLDAGSM
                     QVDRDWHPMEDLIGGALHRLRRLLVKHQVTVEISPALGLVYVDELLLHQVLTNLLENA
                     LRFAPPGSEIKIAAGTIAGKLVLEVMDHGPGIEPGDEERIFEKFYRAPLQPPRSGTGL
                     GLAICRGIVELHGGTIRAENRSGGGAVFRIELPQPPQPALPAEPPPQRAFSDAAK"
     gene            complement(3135..3689)
                     /gene="kdpC"
                     /locus_tag="VFV87_08970"
     CDS             complement(3135..3689)
                     /gene="kdpC"
                     /locus_tag="VFV87_08970"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX5103928.1"
                     /translation="MNDYLISLRLTIFSLVICSAAYPAVILGLASVAAPYKRQGSLVH
                     DQNGTVVGSWLLAQKFTRPEYFWPRPSAVDYNASATGGSNKSPTNPDLTRRAAETLAQ
                     LQPGEGQLVPADLVAASGSGLDPNITLAAARFQAPRVAQARKLSVEKVEELINQHTDS
                     PTLAIFGGEPLVNILLLNLALDDL"
     gene            complement(3781..5937)
                     /gene="kdpB"
                     /locus_tag="VFV87_08975"
     CDS             complement(3781..5937)
                     /gene="kdpB"
                     /locus_tag="VFV87_08975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX5103929.1"
                     /translation="MPKPFPNQQPIQRESTAGPLVSPSLRKKQSLFTAQMLIPSMGQA
                     VKMLDPRIMVRNPVMFVTELGAALTTLVTISAAISGSQEILYFLAVSIWLWLTVWFAN
                     FAEAVAEARGKAQADALKATRQNVTAKKMLEKIPNLSLRPDFHDLVKNGEATFVPAPD
                     LRMEDIVLVEAGDQIPGDGEVIGGVASVDESAITGESAPVIRESGGDRSSVTGGTRVL
                     SDWIFVRITANPGESFLDRMIHLVEGAKRQKTPNEVALTIVLAGFTIVFLLVCSTLYP
                     IARYFDVTIDVPTLAALLVCLIPTTIGALLAAIGIAGMARMIQANVIATSGRAVEAAG
                     DVNVLLLDKTGTITLGNRQAVEFLPAEGITTERLADAAQLASLADETPEGRSIVVLAK
                     EKYGLRGRDLQQLRATFVPFTAQTRMSGVNLDGRRIRKGALEAVAQHLGREVADLPDK
                     VIRDSNKIAQHGGTPLVVAEGEKALGVIHLKDIVKGGIKERFAELRQMGIKTVMITGD
                     NPLTAAAIAAEAGVDDFLAQATPEAKLKLIRQYQGEGHLIAMTGDGTNDAPALAQADV
                     AVAMNSGTQAAKEAGNMVDLDSNPTKLIDVVTVGKQMLMTRGALTTFSIANDVAKYFA
                     IIPAIFMAALPSLAVLNIMHLATPKSAILSAVIFNALVIPCLIPIAIRGVQYRPASAD
                     SLLQRNLLIYGLGGLVVPFIGIKLIDLALFYTGLAG"
     gene            complement(5973..7676)
                     /gene="kdpA"
                     /locus_tag="VFV87_08980"
     CDS             complement(5973..7676)
                     /gene="kdpA"
                     /locus_tag="VFV87_08980"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX5103930.1"
                     /translation="MMVGFLIPALIVGATMVLAWPLGRYMRWAMDPVEPSPRRSRYES
                     VCASVLGVLGGKQGLDWRKCGQNWKQYCLSMLAFNVVMFVLVYTILTTQQWLPLNPDA
                     KGTVEPSLAFNTSASFTSNTNLQHYSGEVSLSYFSQLFALMWLQFVSAATGIAAVTAL
                     SRGLAGRTDLGNFFQDVWRATGLVLLPLALIWASLLALCGMPMTLDGSAIATTLEGAK
                     QTIARGPVAAFVAIKQLGTNGGGWFGPNCTHPYENPTFWSNVLCNMAIIFIPMGTVWM
                     FGRITNRLQHAAVLLGVMLLLYLSFLVSANVFESQPTFAFSDLPVENSMNLEGKELRF
                     GPTAGATWAVSTTITSNGSVNCMHDSLNPLTGLLPLSGMWLNCIFGGVGVGLINLFLF
                     VITAVFLSGMMVGRTPEYLTRKVETKEMKLALIALLVHPLFILVGTALFAVTPWGANT
                     LNNPGSHGLSEILYEFSSAAANNGSGLEGLGDNTVPWNVATGIVMLFGRFIPIIAPLA
                     IAGSLAAKRGTPESAGTFRTDTVLFGVLLIGTILIVGALLFMPVAVLGPIAEHLGTLA
                     H"
     gene            7675..8115
                     /locus_tag="VFV87_08985"
     CDS             7675..8115
                     /locus_tag="VFV87_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103931.1"
                     /translation="MFVLLQNRSSELLRPDEHIAQIAEGESRRRNDACSDEVFHKSSL
                     QPVAGESEAVQQGKTGQADEQHQNPHHGEVLFPATCFCWIADWPPEWQRSILSISVNT
                     TDVRVKLPSKPKRRASRFHQDGLPFLPGRREQRQVYAVLARAVF"
     gene            complement(8140..8967)
                     /locus_tag="VFV87_08990"
     CDS             complement(8140..8967)
                     /locus_tag="VFV87_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103932.1"
                     /translation="MNATGWHLPQFLLASALSSSVANAAAPSESDNFLVISRSSSHSA
                     ALTLKLCEAWRAALLRKWPSDADKAAWSPKCEVHIHNCRKCYLAAVGAGGAQTSASCQ
                     IQWTKNPAQIRRRIDIYNDSPQGLAALPHELTHLVLADLFDAGPPPRWADEGLAVLAD
                     SPGKQALRERDLAQAIARRTEFRLVELLAIEAYPQPSRGAVYCGQCASPARFLLARGD
                     GPRMVEFLKSARVDGYDQALRRVYAIDGVSELKSQWCQWRCPSGATKPAPARGKSAQ"
BASE COUNT         1738 a         2796 c         3025 g         1678 t
ORIGIN      
        1 aacggcttgg tcagatagtc gtcggctccc gcgtcgaggg cggcgacttt gtcggcttcc
       61 tgcccgcggg cggatagcac gatgatcggc acgcgagacc agccgcgaag gtgggcgatt
      121 acctcaaagc cgctaacatc gggcaggccg aggtcgagga ggacgacatc cggcggctgc
      181 gaagccgcgg cgcggatgcc agcctggcca gattccgcct cggccacctg catctggttg
      241 gccgcgagcg aggcccgcag gaatcggcgg atttccggct cgtcttcaac caccaggatg
      301 cgggggaggt cggacacggg gagaaggcag aggtcagagg ccagggatta tttcgccgcg
      361 tcgctgaagg cgcgctgggg cggaggctca gccgggagtg ccggttgtgg cggctgtggc
      421 agctcgatgc ggaacacggc gccgcctcct gaccggtttt cagcccggat cgtgccaccg
      481 tgcaattcaa caattccacg gcagatcgcc aggcccagtc ccgtgccgga gcggggcggc
      541 tggagaggag cgcgatagaa cttctcgaaa atgcgctcct cgtcgcccgg ttcaatgcca
      601 gggccgtggt ccatcacttc gagcacgagt ttcccagcga tcgtccctgc cgcaatcttg
      661 atctcacttc cgggcggggc aaatcgcagg gcattctcaa ggaggttggt cagcacctgg
      721 tggagcaaca gctcatcgac atagacgaga ccgagcgcag gtgaaatctc gaccgtgacc
      781 tgatgcttca cgagtaaccg ccgtaatcgg tggagcgcgc cgccgatcag gtcttccatg
      841 ggatgccagt cgcggtcaac ctgcatgctg ccggcgtcga gtcgcgtcat atcgagcagg
      901 ttggcgatca gccgattgag ccggtcggtt tcggccacga tcgactcggc cagctcgcgg
      961 cgggcatccg ggtgcaaatc gtccgcggct tcggctagcg tgctgccggc gccggcgatc
     1021 gcggccagcg gcgtgcgcag gtcgtgcgag acggcgctga gcagggcgct gcgcagtttt
     1081 tcgcgctcgt agcgaagctg gttttcgcgt gccagctcgg cggattgaat ccgttcgacg
     1141 accagtccga gctggccagc caccgtttcg atcagccgca attgatcagg cgaaaaccgc
     1201 cgaccctcct cggccggccg cacgcccagg acgccgacga tctccccggc cgcaaccagc
     1261 ggtacaaaga gcagtccgac ggacgggagc gtatcggtgc cgagaccggc cgggcatgcg
     1321 tgatcggcga cccaactggc aacggcctgt tcgtgcggtg tcagctcacc gggggaaacg
     1381 cgaatcggct ccagccgcga ctgcgtcttc gacgccgcct tgacggcgac gagcggaaag
     1441 acctggccac agacaaggcg ctggaggcgc tcgcggacgc gctccaggtg cgggagcctg
     1501 tcgatctgga cgagagcccg ggacagctcg ctcagctcgg cagcttcaag ctcgcggcgg
     1561 cgagcgagcg aggcctgaaa cgtcaaccgg ccagtgagcg tgctgataag cagccccgtc
     1621 aacagcatga cgacgaacgt gaagatgtac tgtgtgtcgc tgacggcaaa ggtgtagtaa
     1681 ggatggatga agaagaagtt gaacgccagc acgcccagga aggacgcgag aatcgatggc
     1741 ccgcggccgt gccacgtggc gacggcaacg acacccgcta gatagatcat gcacagcgtg
     1801 accgtactga caaatggaaa gatagcccag caaagccccg tcgccactac aatcgcgacc
     1861 agcgctgcgc cataccccgc cagccgcact ggccgctcgt cgccggcggg atgccttgtc
     1921 gcggccgttg actcgccgct gatcacatag acatcaatgt ctccgcagcg gcgggcaagg
     1981 tcgtaaatga atgagccgcg gatcagctcg cgccagcggg aatgcaaggt cttgccgacg
     2041 atgatcttcg tcacgttgtg ttggcgggcg taggccacga tttcacccac cgagtccgcg
     2101 ccgccgagcg tgacgacctc gccccccaag cggcgggcaa gctggagcgt ttcggctaaa
     2161 cgttcacgat ctgacggccg cagtcgggca tccgtcggcg tttcgacatg aacggcgatc
     2221 cacggcgccc gcagactggc cgccatccgc cgcgtcgccc gcaccaggct cggtgaagtt
     2281 ggtcccggag tgacgcaaac gagcagtcgc tcggctgcag gccaggtttt ctcaattgcc
     2341 tgatcggtgc gatagtccgt catctgccga tcgacgcgct cggccatctt ccgcagcgcc
     2401 agctcgcgga gagcgatcaa gttgcctttg ttaaagaagt gctccagcgc tcgggccgcc
     2461 tggccgggga catagacctt cccttcgcga aggcgcccga tcagatcgtc cggcggcaga
     2521 tcgacgagct cgacctcttc ggcctgctcg aagacgctgt cggggagcgt ctcgcgaacg
     2581 atcacgccgg tgatcttggc aacgacgtcg ttgaggcttt ccaggtgttg cacgttgacc
     2641 gtcgtataga cgtcgatgcc agccgcgagc agggcctcga cgtcttgcca gcgcttggca
     2701 tgcacgagcg gctgctcgtc gtcgatggca ttggtgtggg ccagctcatc gacgacgatg
     2761 agctggggct tcgcggccag gcccgcttgc agatcaagtt ccaggagctg ccggccacgg
     2821 taattgactg tccgcttggg gagcacgtcc aggccgagca ccagggcctg cgtttccggc
     2881 cgggcgtgcg gctcgatata gccgacgacg acgttgacgc cttctttggc caccttgcga
     2941 gccgcttcga gcatcgcata ggtcttgccg acgcctggcg ctgcgccgaa gaagatcttc
     3001 agcttgccgc ggcgggcttt ggcttcctcc gcctgcacgc gggccagcaa ttgatcggga
     3061 ttgggtcggt ctggatcggc catcgggaca ctacgtggtc aagcctcatc ctattccttg
     3121 ggtggcttgg gtcgttatag gtcatctagc gcgagattca ggagcaatat attcaccagc
     3181 ggctcaccgc cgaaaattgc gagtgtgggc gaatcggtgt gctggttgat gagctcctcg
     3241 actttctcca ccgatagctt gcgcgcctgg gccacccgag gcgcttgaaa tctggccgcg
     3301 gcgagcgtga tgttcgggtc caggcccgac ccggacgccg caaccagatc agccggcacg
     3361 agctgcccct cgcccggctg gagctgggcg agggtttcgg cggcccgcct ggtgagatcg
     3421 gggttcgtgg gcgacttgtt gctgccgccg gtggcgctgg cgttatagtc cacggccgag
     3481 ggccgcggcc agaagtattc cgggcgtgtg aatttctgcg caagcaacca ggaaccaacg
     3541 accgtgccgt tctggtcgtg aaccaggctc ccctgacgtt tgtacggcgc agcgaccgac
     3601 gccaagccta agatgaccgc cggatacgca gccgagcaga tcaccaggct gaaaatggtt
     3661 agacgcaggg agatgagata gtcgttcatg agagttgtgt gtgagcgggt gagtggtgag
     3721 ttgcggctgt cagtggtcaa tggtcagttg gaatcgcttg gtttactgtc ccctgtcccc
     3781 ttatcctgcc aggcccgtgt agaacagcgc gaggtcgatc agcttgattc cgataaacgg
     3841 aaccacgagt ccgcccaggc cataaatcag caagttccgc tgcaggagcg aatcggccga
     3901 ggcgggccgg tattggacgc cgcgaatggc gatgggaatc aggcagggga tcacgagggc
     3961 attgaagatt accgccgaga ggatcgcgct cttgggagtg gcgaggtgca tgatgttcaa
     4021 caccgccagg ctcggcaggg cggccatgaa gatcgccggg atgatcgcga aatacttcgc
     4081 cacgtcgttg gcaatgctga acgtcgtgag ggcgccgcgg gtcatgagca tttgctttcc
     4141 caccgtcaca acgtcgatca atttcgtcgg gttgctgtcg agatcgacca tgttgccggc
     4201 ttccttggcg gcttgggtgc cgctgttcat ggcgacggcg acatcggcct gggccagagc
     4261 gggggcgtcg ttggtgccgt cgccagtcat ggcgatcagg tgcccttcgc cctggtactg
     4321 acggatcagc ttcaatttgg cttcgggcgt ggcttgagcg aggaagtcat cgacgcccgc
     4381 ctcggcggcg atggccgccg cggtgagcgg gttgtcgccg gtgatcatca ccgtcttaat
     4441 tcccatctgg cgcagctcgg cgaagcgctc cttgatgccg cctttgacga tgtccttgag
     4501 gtggattact cccagcgcct tttctccctc ggccacgacc agcggcgtgc cgccgtgctg
     4561 ggcgatcttg ttggaatcgc ggatcacctt gtcgggcaga tcggcaactt cgcggcctaa
     4621 atgttgggcc accgcctcca gggcgccttt gcggatccgg cggccatcga gattgacgcc
     4681 gctcatccgc gtttgcgcgg taaacggcac gaaggtcgca cggagttgct ggaggtcgcg
     4741 tccgcgaagt ccatattttt ccttggccag gactacgatg ctgcgcccct ccggcgtctc
     4801 atcggccagc gaggcgagct gggccgcgtc ggccagccgc tcggtggtga tcccttcagc
     4861 cggcaggaat tcgaccgcct ggcggttgcc gagggtgatc gtgccggtct tgtcgaggag
     4921 cagcacgttg acgtcgcctg ctgcttcgac cgcgcgaccg ctggtggcga tcacgttggc
     4981 ctggatcatc cgggccattc cggcgatacc gattgccgcc aaaagcgcgc cgatcgtggt
     5041 cggaatcagg cagacgagca gcgcggcgag cgtcggcacg tcgattgtca cgtcaaaata
     5101 gcgggcaata gggtaaagtg tcgagcaaac caggaggaaa acgatcgtaa acccagccag
     5161 gacgatggtt aacgccactt cgttcggcgt cttctggcgc ttggcgcctt cgaccaggtg
     5221 gatcatccgg tcgaggaagc tctcgcccgg gttggcggta atccgcacga agatccaatc
     5281 ggagaggacg cgcgtgccgc ccgtgacgct gctgcggtcg ccgccgcttt cgcggatgac
     5341 cggggcgctt tcgccggtaa tggcgctttc atcgacgctg gcgacgccgc cgatcacctc
     5401 gccgtcgccg ggaatctggt cgcccgcttc gacgaggaca atgtcttcca tccgcaggtc
     5461 gggggcgggc acgaacgtcg cttcaccgtt cttcaccagg tcgtgaaaat cgggccggag
     5521 cgacagattg gggattttct ccagcatctt cttggcggtg acgttctgcc gcgtggcttt
     5581 gagggcgtcg gcctgggcct tgccgcgggc ttcggcgacc gcctcggcga aattcgcgaa
     5641 ccaaacggtg agccacagcc agatcgagac ggccaggaaa tagagaatct cctggctccc
     5701 cgaaatggct gccgaaatcg tgacgagcgt cgtcagcgcc gctccgagct cggtgacgaa
     5761 catcaccggg ttgcggacca tgatccgcgg gtcgagcatt ttcacggcct ggcccatcga
     5821 ggggatcagc atttgggccg tgaacagcga ctgctttttg cgcaggctcg ggctgacaag
     5881 cgggccagcg gtagattcgc gctggatggg ctgttggttt gggaacggtt tcggcatgga
     5941 ttcagtgggg ttcatggcgt ttccttccaa ctttagtggg cgagggtgcc caggtgctcg
     6001 gcgatcggtc ccagcaccgc cacgggcatg aacaggaggg cgccaacgat caggatcgtc
     6061 ccgattagca ggacgccgaa caggaccgtg tccgtgcgga acgtgccggc tgattccggc
     6121 gtgccgcgct tggccgccag ggagccggca atcgccagcg gagcgatgat ggggatgaag
     6181 cggccgaaaa gcatgacgat gcccgtagcc acattccagg ggacggtgtt gtcgcccagg
     6241 ccttccaagc cggagccgtt gttggccgcc gccgaactga actcatagag gatttcgctc
     6301 agcccgtggc tgccggggtt gttcaaagtg ttcgcgcccc agggcgtgac tgcgaacagc
     6361 gccgtgccaa ccaggatgaa caagggatga accaacaggg cgatgagggc cagcttcatc
     6421 tccttggtct ccaccttgcg ggtcaggtac tcaggcgtgc ggccgaccat cattcccgag
     6481 agaaacaccg cggtgatcac gaacaggaag aggttgatca gtccgacgcc gacgccgccg
     6541 aagatgcagt tgagccacat gcccgaaagg ggcaacagtc cggtgagcgg attcaggctg
     6601 tcgtgcatgc aattcacgct gccgttgcta gtgatcgtcg tcgaaaccgc ccaggtcgct
     6661 ccggccgtgg gaccgaaacg cagctccttg ccctcgaggt tcatggagtt ttcgaccggc
     6721 agatcggaga aggcgaaggt gggctgcgac tcgaagacgt tggccgagac aaggaagctc
     6781 aaatacagca gcaacatcac gccgagcagg acggcggcat gctggagccg gttggtgatc
     6841 cggccgaaca tccagacggt gcccatcggg atgaagatga tcgccatgtt gcagagcacg
     6901 ttcgaccaga aagtcgggtt ctcataggga tgcgtgcagt tcggtccgaa ccagccgcca
     6961 ccgttggtgc cgagctgctt gatcgcgaca aacgccgcca ctggcccgcg ggcgatggtc
     7021 tgcttggcgc cttccagcgt ggtggcgatt gccgagccat cgagcgtcat tggcatgccg
     7081 cacagagcga gcaaggaggc ccaaatcagg gctaagggga gcagcaccag gcccgtggcc
     7141 cgccacacgt cctggaagaa gttccccagg tccgtgcggc ccgctaaacc gcggctgagc
     7201 gcggtgacgg cggctatgcc ggtcgcagcg ctgacgaatt gcagccacat cagggcgaag
     7261 agttgcgaaa agtagctcag cgaaacctcg cccgagtagt gctgcaggtt cgtgttgctg
     7321 gtgaagctgg cggacgtgtt aaaggccagg ctcggctcga ccgttccttt ggcgtccgga
     7381 ttcagcggca gccattgctg cgtcgtcaga atcgtgtaca ccaggacgaa catcaccacg
     7441 ttgaacgcca gcattgacag acagtactgc ttccagttct gtccgcattt tctccaatcc
     7501 agtccctgct tgccccccag cactcccagg acggaagcac agaccgactc gtatcggctg
     7561 cgccgggggc tgggctcgac cggatccatc gcccagcgca tgtaccgacc cagcggccag
     7621 gcgagcacca tcgtggcgcc aacaatcagc gccggaatca aaaagccaac catcatgttt
     7681 gtccttcttc agaatcgcag ttcagaattg ctccggcctg acgagcacat agcacagata
     7741 gccgaaggcg aaagccgtcg tcgcaatgac gcatgcagtg acgaagtatt ccataagtcc
     7801 tccctacagc cggtcgcagg cgaaagtgaa gccgtacagc aaggcaaaac aggccaggcc
     7861 gacgagcagc atcaaaatcc acatcatggc gaggttctct tcccggcgac ttgcttctgc
     7921 tggatcgctg attggccgcc ggaatggcag cgatcgattt tgagtatttc ggtgaatact
     7981 accgatgtcc gcgtcaagtt gccgtcaaaa ccgaagcggc gggcatcaag atttcatcaa
     8041 gatggtttgc cgtttctgcc tgggcggcgg gagcaacgtc aggtttatgc cgtgttggcg
     8101 agagcggttt tctgagcaaa tatgagtggg ttcggctcgc tattgagcgg atttgccgcg
     8161 cgcgggagcg ggtttggttg caccggatgg gcagcgccac tggcaccatt gcgacttcag
     8221 ttcgctcacg ccgtcgatcg catagacccg acgcagtgcc tggtcatacc catcaacgcg
     8281 agccgacttg agaaattcga ccatccgcgg accatcgccg cgagccagca ggaaacgcgc
     8341 cggcgaggcg cattgcccgc aatagactgc cccccgggaa ggttgcggat aggcttcaat
     8401 ggccagcagc tcaaccagcc gaaattcggt tcgccgcgcg atggcctggg ccagatcgcg
     8461 ctcgcgcagg gcttgctttc cgggcgaatc ggccagcacg gccaaccctt cgtcggccca
     8521 gcgtgggggc gggccggcgt cgaacaggtc ggcgagcacc aggtgagtca actcgtgcgg
     8581 cagggcggcc agtccctgcg gcgagtcatt ataaatatcg atccggcggc gaatttgcgc
     8641 gggatttttc gtccattgaa tctggcagct ggcgctggtt tgggcaccac cggcgccgac
     8701 cgcggccaaa taacacttgc gacagttatg gatatgcact tcgcacttgg gtgaccaagc
     8761 agctttatcg gcatcactgg gccatttgcg aaggagtgcc gcgcgccatg cttcgcacag
     8821 cttcaaggtc agcgcagcgc tatgcgacga cgaccggctg ataaccagaa aattgtccga
     8881 ctcgctcggc gccgccgcat tggcgacgga gctgctcagc gcagacgcga gcaggaattg
     8941 cggcaaatgc caaccggtcg cattcatcga tagacctcct tcaggagctt gatcgagaga
     9001 atgccaccgg cagccgcctc aaataggtgt caagactgcg aacattgcat caagaactcg
     9061 tcaacgcatt gaagccggca cttcgcagcg gtggccatgc tcaattccga ggcaactcgc
     9121 aaagcgcatc catacatgtt cggccgacag aggcgttgct gggaagagcg tgcttcggtc
     9181 ccatcgccca cgtcaatgtg cctgaccgag gcgctggggc tgagagcgcc gagccat
//
LOCUS       DASUGB010000466         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1582898, whole genome shotgun sequence.
ACCESSION   DASUGB010000466 DASUGB010000000
VERSION     DASUGB010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582898"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFV87_08995"
     CDS             complement(<1..1043)
                     /locus_tag="VFV87_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5103933.1"
                     /translation="MSYPNQPGQSPFYPNPAPNPFYPPPPQQKPGGSKGVWIVLGVLG
                     GGLLLGVLACCGFVGYLAQPAKASAAAKEPFSYADVPLPAFPDRSQPDNSEPGVVWHE
                     IYLGDDGGYYDPPGHGGYMYLYLPDGQHQPQSLPCILHTGAGTTLLHGVELEGEGNPP
                     EYIPYVKSGYAVLLYDMDGPIYDDEDVEEMRDAYKAFKASRAGLVNARNAIEYLIAKV
                     PEVNPSQIYASGHSSAATHALLLAEHEPRLAGVIAYAPAVDLPKWFAPRLGVLRLLLP
                     GVTDFVTQSSPNTHIARLKCPTLLFQAEDDSICDIRDTRTFADQLRAQGTDVTFISVP
                     TGDHYEAMVNDGLP"
     gene            complement(1053..2291)
                     /gene="fadA"
                     /locus_tag="VFV87_09000"
     CDS             complement(1053..2291)
                     /gene="fadA"
                     /locus_tag="VFV87_09000"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HEX5103934.1"
                     /translation="MTATQTRRASEGPATATRSESEGARRSTTNAVVIDCVRTPIGRA
                     HKDRGWFRDVRSDDLAAACVRALVERTGIDPAEVEDVVLGNTQQTGEQGLNAARSIAL
                     LAGLPVETGGATVNRLCGSSLQALNQAAHSIAAGFENVQIVGGLEHMQHLPMDHGLDI
                     NPKLFQRTSKAALMMGVTAEFLAQTQGISRQEQDAFALRSHQRAAAAHAKGEFKREIV
                     PVYGRDEEGNRFLATGDQCVRPDTSLEALAALEPAFMPKVGTVTAGNSSPLNDGAAAL
                     LVMSEDKADALGLKPLVRVRATAVAGVEPSVMGTGPVPAVRKVVKRAGLTLEDIDLIE
                     LNEAFAAQALACIRMLGLDEKKVNVRGGAIAIGHPLGASGARIATTLINAMVDKGAAL
                     GIATMCIGMGQGIATIFERV"
     gene            complement(2288..>2617)
                     /locus_tag="VFV87_09005"
     CDS             complement(2288..>2617)
                     /locus_tag="VFV87_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5103935.1"
                     /translation="LAEHRQSEHKFSKDELTQRLFLPMLVEATRMLEEKIVRDVRDVD
                     LGLIFGIGFPPFRGGLLFWADTLGTAKIVEMLKPLAPLGERFQPTPMLLDLAASGGKF
                     YPQGGAA"
BASE COUNT          433 a          853 c          869 g          462 t
ORIGIN      
        1 gggaggccgt cgttcaccat cgcttcgtaa tggtcgccgg tggggacaga gatgaaggtc
       61 acatcggtgc cctgggcgcg aagctgatcg gcaaacgtgc gcgtgtcacg gatatcgcag
      121 atggagtcgt cttcggcctg gaagaggagc gttgggcatt tgagccgggc gatgtgcgtg
      181 ttgggcgagc tttgcgtgac gaaatcggtc acgcccggca agagcagccg caaaacgccc
      241 agccgcggag cgaaccactt gggcagatcg accgccgggg cgtaggcgat cacaccggcc
      301 agccgcggct cgtgctcggc gaggagcagg gcgtgggtcg cggcgctgct gtggccggaa
      361 gcatagatct gcgacggatt gacctccggc accttggcga tcaggtattc gatggcgttg
      421 cgggcgttca cgagacccgc ccgcgaagct ttgaaggcct tgtaagcatc gcgcatctcc
      481 tccacgtctt cgtcgtcgta gatcgggcca tccatgtcgt aaagcagcac cgcatagccc
      541 gatttgacgt aggggatgta ttccggcgga tttccttcgc cctccaattc cacgccgtgc
      601 aagagcgtcg ttcccgcgcc ggtgtgcagg atgcagggga gcgactgggg ctggtgctgg
      661 ccgtccggca gatagagata catgtatccg ccgtggcccg gcggatcgta atagccgccg
      721 tcgtcgccca ggtagatttc gtgccagacg acgccgggct cggagttatc cggctgactg
      781 cggtcaggaa atgcgggcag cggcacgtcg gcatacgaga agggttcctt cgcggcagcc
      841 gaggccttcg cgggctgtgc caagtagcct acgaacccgc agcaggcgag aacccccagc
      901 agcagcccgc cgcccagcac gcccagaacg atccacaccc ccttcgagcc accgggcttc
      961 tgctgcggcg gtggcggata gaacgggttg ggagcgggat tgggatagaa cgggctttgc
     1021 cccggctgat tgggatacga catggcgact tcctagacgc gctcaaaaat agtggcgatt
     1081 ccctgcccca tgccgatgca catggtggca atcccgaggg cggcgccctt atcgaccatc
     1141 gcattgatca gcgtcgtggc gattcgcgcg ccgctggcgc cgagcggatg gccgatggcg
     1201 atcgccccgc cgcggacgtt gactttcttc tcgtccaggc cgagcatgcg aatgcacgcc
     1261 agcgcctggg cggcgaatgc ctcgttgagc tcgatcaggt cgatatcttc cagcgtcaac
     1321 ccggcccgct tcacgacctt ccggaccgcc ggcacggggc cggttcccat cacgctgggc
     1381 tcgacgccgg ccaccgcagt ggcccgaacc cgtaccaggg gcttgagacc gagcgcatcg
     1441 gccttgtcct ccgacatgac gagcagcgcc gccgcaccgt cattgagcgg cgagctgttg
     1501 ccggccgtca ccgtgccgac tttgggcatg aatgccggct ccaaggcagc cagggcctcc
     1561 agcgacgtat cgggccggac gcactgatcg cccgtcgcca gaaagcggtt cccctcctcg
     1621 tcgcggccat agaccggcac gatctcgcgt ttgaattcgc ccttcgcatg agccgcagcc
     1681 gcccgctggt ggctgcggag agcgaaagcg tcctgctcct ggcgcgagat gccttgcgtc
     1741 tgggccagga actcggcggt cacgcccatc atcagggcgg ccttgctcgt tcgctggaag
     1801 agcttgggat tgatgtccag gccgtggtcc atcgggaggt gttgcatatg ctccaggccg
     1861 ccaacgatct gcacgttctc gaaacccgcg gcaatcgagt gggcggcctg attcaatgcc
     1921 tgcagactgc tgccgcagag gcggttgacc gtcgccccgc ccgtctcaac cggcagtccg
     1981 gccagcaggg cgatcgaacg ggccgcgttc agtccctgct cgccagtctg ctgcgtgttg
     2041 cccaggacga cgtcctcgac ctccgccggg tcgattcctg tccgctcgac cagcgcccgc
     2101 acgcaggcgg cggccagatc gtcgctccgc acatcacgga accagccgcg gtctttgtgg
     2161 gcgcggccga tgggcgtgcg gacgcagtcg atgacgacgg cgttggtagt cgagcgacgg
     2221 gctccctcgc tttcgcttcg ggttgccgtg gcggggccct cgctggcgcg tcgggtttgt
     2281 gttgcagtca tgcggcgcct ccttggggat agaatttgcc gccgctggcg gccaggtcca
     2341 gcagcatcgg cgtgggctgg aatcgctcac ccagcggcgc gagcggcttg agcatctcga
     2401 cgatcttcgc cgttcccagc gtatcggccc aaaacaataa gccgccgcga aacggcggga
     2461 agccaattcc gaagatcagc cccaggtcca cgtcgcgcac gtcgcggacg attttctcct
     2521 ccagcatccg cgtggcctcg accagcatcg gcagaaacag ccgctgggtc agctcatcct
     2581 tggaaaactt atgctcgctc tggcgatgct cggccag
//
LOCUS       DASUGB010000467         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_888001, whole genome shotgun sequence.
ACCESSION   DASUGB010000467 DASUGB010000000
VERSION     DASUGB010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888001"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFV87_09010"
     CDS             <1..238
                     /locus_tag="VFV87_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103936.1"
                     /translation="GRLQPKARLFVIASDDKFGVRAFPFTRLNSASGGIAVAAWHVGG
                     LLYVVVIDNNNQRLEQFVREKEFALALPPAAPPA"
     gene            372..911
                     /locus_tag="VFV87_09015"
     CDS             372..911
                     /locus_tag="VFV87_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103937.1"
                     /translation="MKIIRIASVVCLLISVKFAAGQELSDVPAEPMYESIMEIRPENN
                     YATPTEPVEVAQAPAPATVLAPPIVRGNAFATEQLLPTPQTVTPDMWYYSQEIQRHDD
                     PAQAVRRKAELRAAQRMQRIAAMKWFGFSNSRPQASPVPMMGMYSPVWIGNGYDRYDW
                     AGVNWAAAPVIRVENYEYR"
     gene            complement(967..1040)
                     /locus_tag="VFV87_09020"
     tRNA            complement(967..1040)
                     /locus_tag="VFV87_09020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1004..1006),aa:Arg,seq:tct)
     gene            1406..2833
                     /locus_tag="VFV87_09025"
     CDS             1406..2833
                     /locus_tag="VFV87_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5103938.1"
                     /translation="MRIAIAAVAVLCLLSYVSNVLSAEPAAKPNIVLIFIDDMGCGDI
                     GPFGNTQWKTPHLDKFAAEGMKFTSFYATPVCSMSRACLMTGCYNARVSIPGVLFPGS
                     QIGLHPDELTLAEVAKQQGYATICVGKWHLGHREPFLPTKQGFDSYFGIPYSNDMTID
                     PEHARFAKNCLFRDDMTEEKARAEAIRHKVPLMRGEEVVEYPADQATLTKRYTAEAVK
                     FIREHRSDPFFIYLPHTMVHLPLAASDEFLGKSGGGLLGDAIEELDWSVGQIMQTLQD
                     TQLDDKTLVIFTSDNGAAVGSSAPWRGKKASNYEGGVREPCIMRWPGKIPAGTSCDRI
                     AGNIDVLPTMAKLIGFEVPKDRVVDGRDITPLMFDPQAEPVRDTHLYFNANQSLAAIR
                     QGDWKFFLRAPQGGGNAKKAKAAKNDGAQAGPVLYDLKSDPGETINVAEKHPDIAVKL
                     RGEATRREAEIKENRRPAGQIASAK"
     gene            2889..>3460
                     /locus_tag="VFV87_09030"
     CDS             2889..>3460
                     /locus_tag="VFV87_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5103939.1"
                     /translation="MRFPLLLALFVATCATPVPAGAIDRPNVVVILADDLGYGDLSCY
                     GHPKFKTPHLDRMAAEGARLTQFNTPMPFCAPTRASLLTGRYPKRCGMNGNPAPDGGE
                     AADSLALPANEVLLPQLFKQAGYATGMVGKWHLGHKRPDQMPTSRGFDEYLGILYSND
                     MRPVQLVEGTKAIEYPVVQATLTRRYTARAL"
BASE COUNT          682 a         1072 c         1043 g          663 t
ORIGIN      
        1 aggccggcta cagcccaagg cgcggctgtt tgtgattgcc tctgatgaca agtttggcgt
       61 aagggccttt ccgtttacac ggctgaattc tgcgtccggc ggaattgccg tcgccgcctg
      121 gcatgtcggc ggcctgctct atgtcgttgt cattgataac aacaaccagc ggctggaaca
      181 gttcgtccgc gagaaggaat tcgcgttggc gcttccgccc gccgcaccac ccgcataacc
      241 gctacagctt gacacgactt tgcgggctgc tccgatcgca atggattcgc ccgtccgact
      301 gtgccgaaag agccggtaac gccacgtttg ccgcctgtgc ggctggttct ttcgccctgc
      361 cggagcttgc catgaagatc attcgtatcg ccagcgttgt ctgcctgctg atcagcgtga
      421 agtttgctgc gggtcaggag ctgtccgacg tgccagccga gccgatgtac gaatcgatca
      481 tggaaatccg accggagaac aattacgcga cgcccaccga gccggtcgaa gttgcccagg
      541 cgcccgcgcc ggcgacggtc ctggccccgc cgatcgttcg cggcaatgcc ttcgccaccg
      601 agcagctttt gcccacgccg cagaccgtga cgccggacat gtggtactac tcgcaggaga
      661 ttcagcggca tgacgatccc gcccaggccg tccgccgcaa agccgaactt cgcgccgccc
      721 agcggatgca gcggattgcc gcgatgaagt ggttcggctt cagcaactcg cgcccgcagg
      781 ccagccccgt gccgatgatg ggcatgtatt cgcccgtctg gatcggcaac ggctacgacc
      841 gctacgactg ggcgggagtc aactgggctg ccgcaccggt cattcgcgtc gagaactacg
      901 agtatcgcta gttgaggcgc gctcggcgat catcgcgcca ggccgcagcc gatttccact
      961 ccaaagcacc cccggcagga ctcgaacccg cgacctaggg attagaaatc ccttgctcta
     1021 tccagctgag ctacgggggc gtggtgaatt cgtcaaatca cgcgcgaatg cgccagcggg
     1081 tacgacgaat ggcaatggat cccaagcgtc atgctgcctc attctacgta gggcatgtgg
     1141 ctgagaaagc ccacgcgccg tcctcagtgg tcttcgcttc tccattggag tcttcgcagg
     1201 agcacaaagg ttcacgacat ttgcccggct ggaacatttc gggcggcgtt cgattccagg
     1261 caattcccaa atcggtgaac gagcggctgc tactcgattc atttgagcac tatcgaccag
     1321 ggcaacggtc gcgtgccatc tgcccttcag gctagaatcc gggtctagac tcgaacctgc
     1381 ccgttccaac gccggaggat gttctatgcg cattgcgatt gccgcggttg ccgtgttgtg
     1441 cttgttgtca tatgtgagca atgttctttc tgcagagccg gcagccaagc caaacatcgt
     1501 gctgattttt atcgacgata tgggctgcgg cgatatcggc ccgttcggca atactcaatg
     1561 gaaaacgccg cacctcgaca agttcgcagc cgaggggatg aaattcacca gcttctatgc
     1621 cacgcccgtc tgctcgatgt cgcgggcgtg cctgatgacc ggctgctaca acgcccgcgt
     1681 gtcgattccc ggcgtgctgt ttccgggaag ccagattggg ctgcatcccg atgaactgac
     1741 gctcgcggag gtcgcgaagc agcagggtta tgccacgatc tgcgttggca agtggcacct
     1801 cggccaccgc gagccgtttc tgccgactaa acagggtttc gactcgtatt tcggcatccc
     1861 ctacagcaac gatatgacga tcgacccaga gcacgcccgg ttcgccaaga attgcctgtt
     1921 tcgcgacgac atgacggaag aaaaggcccg ggccgaggcg attcgccaca aggtgccgct
     1981 gatgcgcggc gaggaagtgg ttgaatatcc ggcagatcag gcgacgctga cgaagcgcta
     2041 cacggcggag gcagtgaagt ttatccgcga gcaccggtcc gacccgtttt tcatctattt
     2101 gccgcacacg atggtccatt tgccgctcgc cgcgtccgac gagtttcttg gcaaaagcgg
     2161 cggcggcctt ctgggcgacg cgatcgagga gctcgactgg tcggtcggcc agatcatgca
     2221 gacgctgcag gacacgcaac tcgatgacaa gacactcgta atcttcacct ccgacaacgg
     2281 cgcagccgtc ggttcgtccg cgccgtggcg cggcaagaag gccagcaact acgaaggggg
     2341 cgtgcgcgag ccgtgcatca tgcgctggcc gggcaagatt ccggctggga catcgtgtga
     2401 tcgcatcgcg ggcaatatcg atgtgctgcc gacgatggca aagctcattg gcttcgaggt
     2461 tcccaaggac cgcgtcgttg atggccgcga catcacgccg ctgatgttcg atccgcaggc
     2521 cgaaccagtg cgtgatacgc atctctattt caacgcgaac cagagcctgg ccgcgattcg
     2581 tcagggagac tggaagttct ttctgagagc gccgcaaggc ggcggcaacg cgaagaaggc
     2641 caaagcggcc aagaacgatg gggcgcaggc cggtcctgtg ctctacgacc taaagagcga
     2701 cccgggcgaa acgatcaacg tcgccgagaa gcaccccgac atcgccgtca aactccgggg
     2761 cgaagccacg cgccgcgaag cggaaatcaa agaaaaccgc cgaccggcgg gccaaatcgc
     2821 cagtgcgaaa tgaagctgct ttgatgtggt tggtgttgat ttcttgtcta ttggctttca
     2881 cgaacatcat gagatttccc ctgcttcttg cgctgttcgt cgcaacctgc gcgacacccg
     2941 tcccagccgg tgcaattgat cgcccgaatg tcgtcgtcat cttggcggac gacctgggtt
     3001 acggcgacct tagctgttac ggccatccca agttcaagac gccgcatctg gaccggatgg
     3061 cggccgaagg ggcacggctg acgcaattca atacgccaat gccgttttgc gctccgactc
     3121 gggcatcgct cttgactggt cgttacccca agcgctgcgg catgaacggc aatccggcgc
     3181 ccgacggcgg cgaggcggcc gacagccttg ccctgcccgc caacgaggtt ttgctcccgc
     3241 aacttttcaa gcaggccggt tacgcgaccg gaatggtcgg gaagtggcat ctaggccaca
     3301 agcggcccga tcagatgccg accagccgcg ggtttgatga atacctcggc atcctgtaca
     3361 gcaacgacat gcggccggta caactcgtcg aagggacgaa ggcgatcgaa tacccggttg
     3421 ttcaggccac gctcacgcgg cgctacaccg cgcgggccct
//
LOCUS       DASUGB010000468         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1303663, whole genome shotgun sequence.
ACCESSION   DASUGB010000468 DASUGB010000000
VERSION     DASUGB010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303663"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFV87_09035"
     CDS             complement(<1..354)
                     /locus_tag="VFV87_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX5103940.1"
                     /translation="MSKSKPDKDAAKKPAKAADKAGERDTKPADGGWRETVESIAMAV
                     ILALLFRGFVAEAFVIPTGSMAPTLRGRHKDVWCPKCGINYQTTASNERTPSGAPTGQ
                     HVTTTTCPICRYTQTL"
     gene            613..2013
                     /gene="thrC"
                     /locus_tag="VFV87_09040"
     CDS             613..2013
                     /gene="thrC"
                     /locus_tag="VFV87_09040"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5103941.1"
                     /translation="MPLTDFAYQRCISPTCGTTCGTEEVRTSCAKCGDLLDVAYDWDR
                     ARPPKSLRYFEEMWCRRHEALRFSGIYRFHELLPFATADSVVTVGEGQTLLQQADSVA
                     QFVGLNPGRLFLQYEGMNPSGSFKDNGMSAAFTHARAIGAKRAACASTGNTSASLAMY
                     CAATRLMKAVIFIGSGKISYGKLSQALDYGALTIQIAGDFDDAMVRVQEVCRDLGMYL
                     VNSVNPFRLEGQKSIMFRVLEALRWDVPDWIVVPGGNLGNSSAFGKAFAELKHLGLID
                     RLPRLAVINAAGADTLYELYEKRGLRWSGGRADMGIVNHYYKELDELGRKASTIASAI
                     EINRPVNLRKCLRALEDMDGVVRQVSDQEILDAKAKIAAGGFGCEPASAASVAGARLL
                     RDQNVIGADERVVCILTGHQLKDPTATVAYHTTDQEQFNKVLGSRGVKRASFANRAVA
                     VPNDLGEIVKAIQLYS"
     gene            2108..>2608
                     /gene="dapB"
                     /locus_tag="VFV87_09045"
     CDS             2108..>2608
                     /gene="dapB"
                     /locus_tag="VFV87_09045"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5103942.1"
                     /translation="MTTTIAIHGAAGRMGRRLIALASTDPELKIMAAIDSPANPEIGK
                     DAGLLAGVNELRLLLAGALNVQPQVVIDFSIPEATEKILALCVNRQIPVVVATTGLND
                     AQQSQVVEASETIPVVFSPSMSTTVNLAMKLCATAGEVLKDMASGVDVEIIERHHRMK
                     EDSPSGT"
BASE COUNT          463 a          803 c          847 g          495 t
ORIGIN      
        1 gagcgtctgg gtatagcggc agatggggca ggtggtggtc gtgacgtgct ggccggtggg
       61 ggcgcccgat ggcgtccgct cgttgctggc ggtcgtctga tagttgatgc cgcacttggg
      121 gcaccagacg tccttgtgcc ggccccgcag cgtgggggcc atcgagccgg tggggatcac
      181 gaacgcctcg gccacgaacc cgcgaaagag cagggcgagg atgaccgcca tcgcgatcga
      241 ctcgaccgtt tcccgccacc cgccgtcggc tggtttggtg tccctttcgc cggccttgtc
      301 ggcggctttc gcgggcttct tggcggcgtc tttatcgggt ttcgattttg acatgggaat
      361 ctcgtggggc gggcgtctcg tcggccctgt aacttgactg gtcgctgaac gtggatttca
      421 gtaattcgct cggagaggga aatatatcga aacggcggga gggcgggaac tttggaaatt
      481 ggtcggccgg tgctgtgaga accgcgacca gcggtcgcgg ccttggcgtc gcccttgccg
      541 taatatccca ccccacctag acttacaacc gctcgctgtc agccttctgt cctctgtcct
      601 gtctcctgcc tcgtgcccct caccgacttc gcctaccagc gctgcatcag cccgacctgc
      661 ggcacgacct gcgggacgga ggaggtccgc acgtcgtgcg ccaagtgcgg cgacctgctg
      721 gacgtggcct atgactggga ccgggccagg ccgccgaaat cgctccgcta tttcgaggaa
      781 atgtggtgcc gacgccacga ggcgcttcgg ttcagcggca tttaccgttt tcacgagttg
      841 ctgccgttcg cgaccgccga ttcagttgtt acggttggcg aagggcagac tctgctccag
      901 caagccgatt cggtcgccca attcgtcggc ctgaatcctg gccggctctt cctgcaatac
      961 gaggggatga acccctcggg ttcgttcaaa gacaacggca tgtcggccgc ttttacgcac
     1021 gcccgggcga tcggcgccaa gcgggcggcg tgtgcttcca ccggcaacac cagcgcgtcg
     1081 ctggccatgt actgcgccgc gacgcggctg atgaaggcgg tgatcttcat tggctcgggc
     1141 aagatcagct atggcaaact gtcgcaggcc ctcgactacg gggcgctcac catccagatc
     1201 gccggcgact tcgacgatgc catggtccgc gtgcaagagg tctgccgcga cctgggaatg
     1261 tacttggtga acagcgtcaa tcccttccgg ctggaggggc agaagtcgat catgttccgc
     1321 gtgctggagg ccctgcgctg ggatgtgcct gactggatcg tcgttcctgg cgggaatctc
     1381 ggcaattcaa gtgccttcgg caaagccttc gccgagctga agcacctggg cctcatcgat
     1441 cgcctgccgc gattggcagt gatcaacgct gccggtgccg acacgctcta tgagctttac
     1501 gagaagcgtg gcctgcggtg gagcggcggc cgggcggaca tggggatcgt caatcactat
     1561 tacaaagagc tggacgagct gggacggaaa gcttcgacga tcgccagcgc catcgagatc
     1621 aaccggcccg tgaacctgcg caagtgcctg cgtgccctgg aggacatgga cggcgtcgtg
     1681 cggcaggtca gcgaccagga aattctcgac gccaaggcca aaattgccgc cggcggcttc
     1741 ggctgcgagc cggccagcgc cgcgagcgtc gccggcgctc ggctgctgcg cgatcagaac
     1801 gtgatcggcg ccgatgagcg ggtcgtctgc atcctcacgg gtcatcagct caaagacccc
     1861 accgccaccg tcgcctatca caccaccgat caagaacagt tcaacaaagt cctcggcagc
     1921 cgcggcgtca agcgtgccag tttcgccaac cgcgccgtcg ccgtgcccaa cgacctgggc
     1981 gagattgtga aggcgattca gctttactcg tgaaacagca ggaggtcaga gagcagaggt
     2041 cagaggtcag aagcgcgtca tccgatttcc gacctctgac ctctaatctc tcctctagag
     2101 gccgacagtg accaccacca ttgccattca cggtgccgcg ggacggatgg gccggcggct
     2161 gattgcgcta gcgagcaccg atcccgaact caagatcatg gcggcgatcg attcaccggc
     2221 gaatccggag attggcaaag acgccggcct gctagcgggc gtcaatgagc tgcgcctgct
     2281 gctggccgga gcgctcaatg tccagcctca ggtcgtgatt gatttttcca ttccggaagc
     2341 gaccgagaag atcctggcgc tgtgtgtgaa tcgccagatt ccggtggtcg tcgcgacgac
     2401 gggcctcaat gacgcccagc agtcgcaagt cgtcgaagcg tccgagacga tccccgtcgt
     2461 cttctcgccc agcatgagca cgacggtcaa cctggcgatg aagctttgcg ccacggcggg
     2521 cgaggtgctc aaggacatgg ccagcggtgt cgatgtggag attattgagc ggcatcaccg
     2581 gatgaaggag gattcgccca gcggtacg
//
LOCUS       DASUGB010000469         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_888157, whole genome shotgun sequence.
ACCESSION   DASUGB010000469 DASUGB010000000
VERSION     DASUGB010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888157"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..571)
                     /locus_tag="VFV87_09050"
     CDS             complement(92..571)
                     /locus_tag="VFV87_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103943.1"
                     /translation="MALLGGGTNWSSVFGNERAAHPGSGCCAECGCQCSCRKVCQLIY
                     EKKQVPKISYRCECEDFCVPGPSQRCKVPCDCCDGCCKHKDHIVYTPTCAEVRTRHKV
                     VRIEETEEVCKPKWVVRYLCEQCAQSCPQAERAPGPAAAQAPAVTDLFSTLFSRSTD"
     gene            965..1399
                     /locus_tag="VFV87_09055"
     CDS             965..1399
                     /locus_tag="VFV87_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5103944.1"
                     /translation="MAESRLATVRLVDEQGAGGKVREIFDDIKRTKGIDFVPNFWRAL
                     ATNPDHLELVWRQLKALMHPEAAGRGSRLDAKTREIIALTVSASNGCSYCVNSHTAAL
                     RKLGVDAETLGEVLAVAALFNQTNALADGYQVEPDVLPPLEC"
     gene            complement(1396..1551)
                     /locus_tag="VFV87_09060"
     CDS             complement(1396..1551)
                     /locus_tag="VFV87_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103945.1"
                     /translation="MADELDTWLLSYKTAEKLSNPDSNFDAQASSAGAWRKKTLKKIA
                     PPAPARR"
     gene            1711..2094
                     /locus_tag="VFV87_09065"
     CDS             1711..2094
                     /locus_tag="VFV87_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX5103946.1"
                     /translation="MLVLTRKLQQQIQIGENITVTILRVKGHTVRVGIEAPRDVRVVR
                     GELPRKDVGASAEDQEATIIEARVSVTDEPADAAEEAACSSADQTAGETSAPLVTPTL
                     PPRRVLSRLGNAPLRQIMTNAALAK"
     gene            complement(2494..3687)
                     /locus_tag="VFV87_09070"
     CDS             complement(2494..3687)
                     /locus_tag="VFV87_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103947.1"
                     /translation="MVANTAQPAAPRRRFAPRFSLAAALVAMALVALGLWYWFRVPFE
                     VVHETKSAKEVESVRRTWGGTVRHGPRRVYVAGQLMLVENYRDGLAHGRWEWLDGAGK
                     AHIVAEFRRGELQSFQASPECDQRLAKLLAEGAINDPKLVLTLFKPTKITFRGTPLLD
                     AIQILKDVQQFEFNGTLGKPAGFVEGGFGPKMVRFIPISEEDRQRLLDDRKAERAILE
                     LVRQESGSTESLELVEQWYVPITCEVSDKPLVVALGSILQPHGLVCDYRYGLLWLSEP
                     EEAERWKDPTGVSEIVPPPGSRLAAAWEDESHVEFIETPLLAACDFLSSYHGGIVKFD
                     CSRLPQEYQQGTTRDSLVTANVKGIAFKNVLGLVLEQTRCQARLEGEKIVIEPQPGNP
                     LADNP"
BASE COUNT          781 a         1157 c         1231 g          748 t
ORIGIN      
        1 cccttcgccg tgcgtacgac tagttgccgc tttatccagg ccaccggcca gaagctgcat
       61 ccctgcgcca cagccgaagc cccgccagcg atcagtcggt cgatcgggaa aagagcgtgg
      121 aaaataaatc cgtcaccgcc ggcgcctggg cggcggccgg cccgggagcg cgttccgcct
      181 ggggacaaga ttgggcacac tgctcgcaga ggtaccggac gacccacttg ggcttgcaga
      241 cctcttcggt ctcttcaatt cgcaccacct tgtgccgcgt tcgcacttcg gcgcaagtgg
      301 gggtataaac gatatggtcc ttgtgtttgc aacagccgtc gcagcaatcg caagggacct
      361 tgcagcgctg gctggggccg ggaacgcaga agtcctcgca ctcacagcga tagctgatct
      421 tgggaacctg cttcttctcg tagatgagct gacagacctt gcggcacgag cactggcaac
      481 cacactcggc acagcaacct gagccgggat gggcggcacg ttcgttgccg aaaacactgc
      541 tccaattcgt cccgccaccg agcagtgcca cgcagcaggc gaagatccca gtggccacca
      601 accgtcgcgc tctttttggc actgaatttc ctcaatactc aaggtgcggc ttggaaagaa
      661 ctagcctgga ctgcgaatgg ctcacgtttg ggcggctcgt tctggaaggt acaatcaaca
      721 agatcggcac aaacgataat gtccaataat cagatcggca tacgattggc agttgaagat
      781 tacattggcg agagagtgag atattcgatt taccagcgtt gctagcgttt tgcccgccat
      841 cggcacggga ggctgattga ggctgccggt tcgaacttcg agccggcagt ggtccgtggg
      901 cagtcggact ggcttagaat gcagcttcgc gattgcagca gcaccgtccg tccggagaac
      961 gcccatggcc gaatcacgcc tggccaccgt ccgcctggtc gatgagcagg gagcaggggg
     1021 caaagtccgc gagatcttcg acgacatcaa gcgcacgaaa gggatcgact tcgtccccaa
     1081 tttttggcgg gcgctggcga ccaatcccga ccatctggag ctcgtctggc ggcaactgaa
     1141 ggcgctgatg cacccggaag cggctggccg tggcagtcgt ctggatgcca aaacacgcga
     1201 gatcatcgcg ctgaccgtat ccgccagcaa cggctgcagc tactgcgtga actcgcacac
     1261 ggcagcactg cggaagctgg gggtcgatgc agaaacgctg ggcgaggtgc tggccgtggc
     1321 cgcgttgttc aatcagacga acgcgctggc cgacggctac caggtcgagc cggacgtgct
     1381 accgccactg gagtgctagc ggcgcgccgg cgctggcggg gcgattttct tgagcgtttt
     1441 cttccgccag gcgccagcag agcttgcctg cgcgtcaaag ttgctgtcgg ggttggaaag
     1501 cttttcagcc gttttgtaac tgagtaacca agtgtccagt tcatcggcca cgggggcgaa
     1561 tctcgcaacc ggcgatcgcg aaacaagtta ggtcgatgtc caaaaaatgt gcccgcgttg
     1621 gcacgctccc tgcaacgtgc cgtctatcat tcccgcagcc tgccagaact ttactacgga
     1681 gtcttttcct aacggacaaa ggagtgtgcc atgctcgtgt tgacgcgcaa gctgcagcag
     1741 cagattcaga tcggcgagaa tatcacagtg acgatccttc gcgtgaaagg acatacggtg
     1801 cgggtcggca tcgaagcccc gcgcgacgtc cgcgtcgtcc gcggcgagct gccgcgaaag
     1861 gacgtcgggg cttccgccga ggatcaggaa gcgacgatca tcgaagcccg cgtatctgtc
     1921 accgacgaac ccgcggatgc cgccgaagaa gcggcctgct cctcagccga tcaaaccgct
     1981 ggagaaacct cggcgccgct cgtaactccc actctgcccc cgcggcgcgt gctctcgcgc
     2041 ctgggaaacg ccccgttgcg gcagatcatg accaacgcgg cgctggcgaa gtgatgattt
     2101 ccggatcgga gatcgcagcc acgtgaatcg gctgccgaaa atcgcggcgc aggcttgttc
     2161 cgcctttgtc cccgcggagg ggtcctcgca caattctcgt tgaagaaccc tcagcggcta
     2221 tcagcaaaag cagcttccgg cagcggctgg aggacgatct tttcgccgtc caagcgggcc
     2281 tggcagcggg cctggtcgag catggcgccc aaggcatgct taaacggcag cctggcaatt
     2341 tgcagggtga tggggtggtg gccagcatat tggccgtccg gtggagcagg aacctgcgag
     2401 atcaaactcg atgttgtgct gatattccag aatgccgccg gcgctggtca tccattgtga
     2461 gccaagtcga atgggattac gacgccggta ccgtcagggg ttgtccgcca aggggttgcc
     2521 cggctggggc tcgatcacga tcttttcacc ctccagccgg gcctggcagc gcgtttgttc
     2581 gaggacgagg ccgaggacgt ttttgaaggc gatgcctttt acgttagcgg tgacgaggga
     2641 atcgcgagtt gtgccctgtt gatattcctg cggaaggcgc gagcagtcga atttgacgat
     2701 tccaccgtga tagctcgaga gaaagtcgca agccgcaagg agcggcgttt cgatgaactc
     2761 gacatgggac tcgtcttccc aggccgcggc gagtcttgat cctggcggcg ggacaatttc
     2821 gctgacgccg gttgggtctt tccagcgctc ggcctcctcc ggttcggaaa gccagagcag
     2881 gccatagcgg tagtcgcaga ccaggccgtg cggctggaga atgctgccca aggcaacaac
     2941 caggggttta tcggacactt cgcacgtgat gggcacatac cactgttcca ctaactctaa
     3001 agattccgtt gaaccagact cttgacgcac taattccaaa atggctcttt cggccttccg
     3061 gtcgtccaga aggcgctggc gatcctcttc gctgatcgga atgaaccgga ccattttggg
     3121 tccgaatcct ccttcaacga accctgcagg ctttcccaag gtgccgttga actcgaactg
     3181 ttgcacatcc ttgagaatct gaatcgcatc caatagtggc gtcccgcgaa atgtgatctt
     3241 ggtcggcttg aagagcgtca gtacgagctt gggatcgttg atcgcccctt cggcgaggag
     3301 ttttgcgagg cgctgatcgc attcggggga ggcttggaac gactggagtt cgccgcggcg
     3361 gaattcggcg acgatgtggg cctttccggc gccgtcgagc cattcccagc ggccgtgggc
     3421 gaggccgtcg cggtagttct cgaccagcat gagctggcca gcgacataga cgcgccgcgg
     3481 cccatgccgc accgtcccgc cccacgtgcg gcggaccgat tcgacctctt ttgcgctttt
     3541 ggtctcgtgt accacctcaa acggcacgcg gaaccagtac cagaggccga gcgcgaccag
     3601 cgccatcgcc accagcgccg cggccaggct gaaccgcgga gcgaagcggc ggcggggagc
     3661 ggcgggctgg gcggtgtttg cgaccatctt gcggtcctgg ccagcggagg gaaagagcgg
     3721 gcgtgcgatt ctggcagaag cgtcggcacg cattgccctc attgtgcggg gaggtaatgc
     3781 ggttggcgat aggctggcag tctgagcctg cccccgggaa atgcttgacg cgcacggatg
     3841 aatcgtatat aatgaacaaa agtattaaca actcacaggg cggtgctgcg cgctcagcca
     3901 caagaaggat gaacatg
//
LOCUS       DASUGB010000470         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1442086, whole genome shotgun sequence.
ACCESSION   DASUGB010000470 DASUGB010000000
VERSION     DASUGB010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442086"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFV87_09075"
     CDS             <1..1080
                     /locus_tag="VFV87_09075"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX5103948.1"
                     /translation="LVVWLLGTALMMVFGGGLLRSLQRSYWIRGLNTKPCAIVGVTEL
                     GIQLARNIVDTPELGLKLAGYYDDRPPGRSPQLPPGLKQRIGNIDDLVADARAGFIER
                     IYITFPMRAEDRIRGVLTKLADTTASVYLVPDFFVFQMLHSRWTDIGGLPVVSVFENP
                     LYGVDGLAKRAFDFVVGSLLLTALAIPMAIVALLIKLTSRGPVFFRPRRYGLDGQEIL
                     VWKFRSMTVCEDGHSVAQVTKSDSRVTRIGAFIRKTSIDELPQLFNVVSGSMSLVGPR
                     PHAAAHNEQYRKLIEGYMLRHKVKPGITGLAQVRGWRGETDTLEKMQKRVECDHEYIR
                     EWSLWQDIKILFRTIFVVLKRENAY"
     gene            complement(1101..1592)
                     /locus_tag="VFV87_09080"
     CDS             complement(1101..1592)
                     /locus_tag="VFV87_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103949.1"
                     /translation="MFFVDAEVVPAFRKLLDPKAGAWPNYSYFEQLRKELGSNFGYAV
                     VDSDGDYILDLAGFERCNPNQKLQRKRRTTEDRYRKVVRQMKTFICERCMEEELREHP
                     QLGSGDWTEEWFTAMAALAQEQGWVMADPTKQRESWFYEEFEVVGPKCAASIKTSEKK
                     PAK"
     gene            complement(1837..>2757)
                     /locus_tag="VFV87_09085"
     CDS             complement(1837..>2757)
                     /locus_tag="VFV87_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5103950.1"
                     /translation="WMPNCFGGRENLEAIDFLKKFNEEVHLQYPGVLTIAEESTAWTG
                     VSRPTYVGGLGFSLKWNMGWMNDTRQYMHKEPIHRKFHQDELTFSLIYAFTENFCLPL
                     SHDEVVHGKQSLLAQMPGDLWQRFANLRLLFSYMWTHPGKKILFMGSDFGQWNEWNHD
                     AELQWDLLQWETHGGVKKMVGDLNALLKREPALHQVDFEQGGFEWIECNARDDSVLAY
                     IRRAKDPDDFVVVVCNFTPVPRQNYPIGVPQLGWYQEIFNSDSQFYGGSNLGNFPGLA
                     AEEPGWHYKPARLTVTLPPLATVVFKPERR"
BASE COUNT          512 a          871 c          812 g          562 t
ORIGIN      
        1 ctggtcgtat ggcttctagg aacggcgcta atgatggtct tcggtggcgg gctgctcaga
       61 tcgctccagc gctcgtactg gattcgcggc ctgaacacca agccctgcgc gatcgtcggc
      121 gtgacggagt tgggcatcca actcgcccgc aatatcgtcg atacgcccga actgggcctg
      181 aagctcgccg gctactacga cgaccgcccg ccgggacgct ctccgcagct tccgcccggc
      241 ctcaagcagc ggatcggcaa catcgacgac ctggtggccg acgcccgggc cggattcatc
      301 gagcggattt acatcacgtt tccaatgcga gccgaagacc gcatccgcgg cgtgctcacc
      361 aaactcgccg acaccacggc cagcgtttat ctcgtgccgg acttttttgt cttccagatg
      421 ctgcactcgc ggtggaccga tatcggcggc ctgccggtcg tcagtgtctt cgaaaacccg
      481 ctgtacggcg tcgatggcct ggccaagcgg gcgtttgact tcgtcgtcgg ctcgctgctg
      541 ttgaccgcgc tcgccattcc gatggcgatc gtcgccttgc tcatcaagct caccagccgc
      601 gggccggtct tcttccgccc gcggcggtac ggcctggacg ggcaggaaat cctggtctgg
      661 aagttccgca gcatgaccgt ctgcgaagac ggccacagcg tcgcccaggt caccaaatcc
      721 gacagccgcg tgacgagaat cggggccttc atccgcaaga cgtcgatcga cgaattgccg
      781 cagcttttca acgtcgtgag cggcagcatg tcgctcgttg gcccgcggcc gcacgccgcc
      841 gcccacaacg agcagtatcg caagctgatc gaaggctaca tgctccgcca caaggtcaag
      901 ccgggcatca ccggcctggc ccaagtccgc ggctggcggg gcgagaccga cacgctggag
      961 aagatgcaaa agcgcgtcga gtgcgaccac gaatacatcc gcgagtggtc gctctggcag
     1021 gacatcaaga tcctcttccg cacgatcttc gtcgtgctga agcgggagaa tgcgtattga
     1081 tcagtcgagc gattatttac ctatttggcg ggctttttct cactggtctt aatggaagcc
     1141 gcacacttgg gtcccacgac ctcgaattct tcgtagaacc aggactcccg ttgcttggtc
     1201 ggatccgcca tgacccagcc ctgctcttgc gcgagggcgg ccatcgccgt aaaccattct
     1261 tccgtccagt cgcccgaccc gagctgggga tgttctctca gctcttcctc catgcagcgt
     1321 tcgcaaatga acgtcttcat ttggcgcaca accttcctgt agcgatcctc ggttgtgcgg
     1381 cgtttgcgtt gtaatttctg attgggatta catcgttcaa atccggccaa atccagaatg
     1441 tagtcgccgt cgctatcgac aaccgcgtag ccgaaattgc tgccgagttc tttccgcagt
     1501 tgctcgaagt acgagtagtt cggccatgca cctgccttcg gatcaagcag cttgcggaaa
     1561 gcaggcacta cctccgcatc gacgaaaaac atctagcaca tatcgacaga gacgagttgc
     1621 gatttccgca ctctgtgatc agccacagtc gccagccgga tggcttgacg atcgaggcgg
     1681 taaagtcccg cttggaacct tggaacgacg tactccgcac cctcgtcctg aaactcaaat
     1741 accctcagcc gcggtgaccg gagctcgaaa tagacaaagt cgtcatcgtg ccacggtgtc
     1801 gtgcccctag ccatatcgaa ctccgcttcg gctcccctac ctccgctccg gcttgaagac
     1861 gacggtcgcc agcggcggga gggtgacggt gaggcgggcg ggcttgtagt gccagccggg
     1921 ctcctcggcg gccaggccgg ggaagttgcc caggttgctg ccgccgtaga actgcgaatc
     1981 gctgttgaaa atctcctgat accagccgag ttgcggcacg ccgatcgggt agttctgccg
     2041 cgggaccggc gtaaagttgc agacgacgac gacgaagtcg tcggggtctt tcgcccggcg
     2101 gatgtaggcc agcacgctgt cgtcgcgggc gttgcactcg atccactcga agccgccttg
     2161 ctcgaaatcg acctggtgca gggccggctc gcgcttgagc agcgcgttga gatcgccgac
     2221 catcttcttc acgccgccgt gcgtttccca ctgcaacagg tcccactgca gctcggcatc
     2281 gtggttccat tcgttccact ggccgaagtc gctccccatg aacaggatct tcttgcccgg
     2341 gtgcgtccac atatagctga acagcagccg caggttcgcg aaccgctgcc acaaatcgcc
     2401 cggcatttgg gccaggagcg actgcttgcc gtgcacgact tcgtcgtgcg agagcggcag
     2461 gcagaagttc tcggtgaagg cgtagatcag ggagaacgtg agttcgtcct gatggaactt
     2521 gcggtggatt ggttccttgt gcatgtactg gcgggtgtcg ttcatccaac ccatgttcca
     2581 cttgagcgag aagccgagcc cgccgacgta ggtcggccgc gagacgccgg tccaggcggt
     2641 cgactcctcg gcgatcgtca gtacgccggg atactgcaaa tgaacttctt cgttgaactt
     2701 tttgaggaag tcgatcgcct cgaggttttc gcggccgcca aagcagttcg gcatcca
//
LOCUS       DASUGB010000471         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1999528, whole genome shotgun sequence.
ACCESSION   DASUGB010000471 DASUGB010000000
VERSION     DASUGB010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999528"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2739)
                     /locus_tag="VFV87_09090"
     CDS             complement(<1..>2739)
                     /locus_tag="VFV87_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5103951.1"
                     /translation="PLAEFWRPASAGPGRPGFADGHCGTLAYLRAVWERQTCYPDCSL
                     GEDVAFLRPALVAGCRFEPVPAELDFVYVRHGGNVSDLAGSWQEQGEEMSQVPRELAG
                     VLPLYQRLGPQLAKPRQPAQLGRAERLAPDGHWLDQLRAQAPRRAESVQRCQLEPPLA
                     ELRGPPRPRPPHWDLQPEVIEAHRRLLDEAVGEALASSPPTTSEGRGIVTLGGTSKYF
                     AAAWVMVSVLRKLGCTLPVEWWYLGPEELDPKMVRLAQSLPGVTCIDLRQRLAQVGRT
                     PRMLGCWEAKVWAIMFSRFAEVLFLDADQVPVRNPAYLFDDPAYRRAGAVFWPDFLPF
                     GWTVDADAFGVAGLPLPGNRRRPDWRNPTDYEPFETGQILVEKSRHWPALELTRHIND
                     HSDFWFPAAAKGHHAWHIYGDKDTFFLAWQRLRASFAMPSGPIFTGSDKAGAFIQQDL
                     AGQPIFQHRVQPATKWVLHGENHQPPVFQHHELCLAALDELRRRWPGHPYEPGDEDER
                     ERELSGSLSGRHIWFRAPSQPASIELASQGRTGDPLLHWSVRTFGGSRPEGVLVIASR
                     DKALAFLGRDHWGQWVNHGTQDFLTPAPPAGFELPLAREELAMFNDIVRLNEYRLPDR
                     FDARHFIIDVGGHCGSFAFACLARGVGRVVSIEPIPANFALLQQNLRSFGERSSVLNA
                     AVWPTPGTVRLEQKEGARHSGGWSVIGTTAGIPAVTINLDDLVTAPVRLIKLDCEGAE
                     WPILGAFTRWDLVEAWCGEYHHTQLDVARDGLRAIFEPHGYEVTVEGHPFAAELGHFW
                     AARPPSVQKKALLVLGPESSGTRYLTHLIESSGVWRDPGNCAPPRLPNDWHVVVPLSA
                     PSRIVLSRSLPVAGVWFDPQEVLAQLRAAGYEPTVLIPVRAQPAAEASQAFRGHAS"
BASE COUNT          497 a          882 c          917 g          443 t
ORIGIN      
        1 tactggcgtg accgcgaaac gcctggctgg cctcggcggc aggctgcgcg cggacgggaa
       61 tgagcacggt cggctcgtaa ccagcagcgc gcagttgcgc cagcacctct tgcggatcga
      121 accagacgcc tgccacaggc agcgagcggc tgaggacgat ccgggacggc gcggacaacg
      181 gtacgacgac atgccaatcg ttgggcaacc ggggcggtgc gcagttgcct ggatcccgcc
      241 agacaccgga cgactcgatg aggtgtgtga ggtaacgtgt cccggaactc tcgggtccca
      301 acaccagcag tgcctttttc tgtacgctcg gcggacgagc ggcccagaag tgtcccagct
      361 cagcagcgaa ggggtggcct tctaccgtga cttcgtagcc atgaggctcg aagatcgccc
      421 tcaggccgtc gcgggccacg tcgagctggg tgtggtggta ctcgccgcac caggcttcga
      481 ccagatccca gcgcgtgaag gcgcccagga tgggccactc ggccccttca caatcgagct
      541 tgatcagtcg gaccggcgcg gtgacgagat catcgagatt gatcgttacg gctggaatgc
      601 ccgcagtggt gccaatcacc gaccagccgc cggagtgcct ggcgccctcc ttttgctcaa
      661 gtcgcacggt gcctggtgtt ggccagacgg cggcattgag cacggaggac cgctcgccga
      721 acgatcgcag attttgctgc aggagggcga agttggcggg gatcggctcg atgctcacga
      781 cgcgcccgac accgcgcgcc aggcaggcaa aagcgaagct gccgcagtgg ccgccgacgt
      841 cgatgatgaa gtgtcgcgcg tcaaatcggt ccggaagtcg atattcgttt aaccgcacga
      901 tgtcgttgaa catcgccagt tcctctctgg caagcggcag ctcgaagccg gcgggagggg
      961 ctggggtcag gaaatcctga gtcccgtggt taacccactg gccccagtga tcgcggccga
     1021 ggaaagccag ggctttgtct cgcgaggcga tcaccagaac gccttctggc cgggagccgc
     1081 cgaaggtgcg caccgaccag tgcagcagcg gatcgccagt tcttccttgg gaagctagct
     1141 cgatcgacgc gggctggctt ggcgcgcgaa accagatgtg acggccggag agcgacccgc
     1201 tgagctcccg ctctcgctca tcttcgtcgc ctggctcata gggatggccc ggccagcgcc
     1261 gacgcagctc gtcgagggcc gccaggcaca gctcgtgatg ttgaaagacg ggcggctggt
     1321 ggttctcgcc atgcaagacc cacttggtgg ccggctgcac gcggtgttga aaaatcggct
     1381 ggccggcgag atcctgctgg ataaacgcac cggctttgtc gctgccagtg aaaatcggcc
     1441 cgctcggcat cgcgaaggaa gcacgcaacc gctgccaagc aaggaagaac gtgtccttgt
     1501 cgccatagat gtgccatgcg tggtgtccct tggccgcggc cgggaaccaa aagtcgctgt
     1561 ggtcgttgat gtgccgcgtc agttcgagag cgggccagtg acggctcttc tcaacaagta
     1621 tttgccccgt ttcgaacggc tcgtagtcgg tcgggttgcg ccaatccggg cggcgtcgat
     1681 tgccgggaag cggcaatccg gcgaccccga aggcgtccgc gtctaccgtc cagccgaagg
     1741 gcaaaaaatc cggccaaaag acggccccgg cccggcgata ggctggatcg tcgaacagat
     1801 acgctggatt gcgcacaggg acttggtcgg cgtccagaaa caggacttct gcgaagcggc
     1861 tgaacatgat cgcccagacc ttcgcctccc agcagcccaa catgcgcggg gtgcgtccga
     1921 cctgggcgag gcgctggcga aggtcaatgc atgtcacccc gggcagcgac tgcgccaggc
     1981 ggaccatctt ggggtcgagt tcttccgggc cgagatacca ccactcgacc ggcagcgtgc
     2041 agcccagctt tcgcagcact gagaccatca cccacgcggc ggcaaagtac ttgctcgtac
     2101 cgccgagggt cacgatgccc cggccctcgg aggttgtcgg cgggctactc gcgagcgctt
     2161 cgccgaccgc ctcatccagc agccggcgat gcgcctcgat cacttcgggc tgcagatccc
     2221 agtggggcgg cctggggcgg ggcggtccgc gcaattctgc cagcggcggc tcaagttggc
     2281 agcgttggac tgattcggcg cggcgcgggg cctgagccct cagctggtcc aaccaatggc
     2341 catccggcgc gagtctctcc gcgcggccaa gctgtgcggg ttgccgcggc ttggcgagtt
     2401 gcggcccaag ccgctgataa agcggcagga cacctgccaa ttcgcgcggc acttgcgaca
     2461 tctcttcgcc ctgctcctgc caactgccgg ccaggtcaga aacgttgccc ccatgccgca
     2521 catagacgaa gtcgagttct gcgggaacag gctcgaagcg gcaacccgcg actaacgctg
     2581 gccgcagaaa cgccacgtcc tcacccagcg agcaatcggg atagcacgtc tgccgttccc
     2641 atacggctcg caggtaggcg agcgtgccgc agtgtccgtc cgcgaagcca gggcgtcccg
     2701 ggccggctga tgcgggccgc cagaactccg cgagcggca
//
LOCUS       DASUGB010000472         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1165609, whole genome shotgun sequence.
ACCESSION   DASUGB010000472 DASUGB010000000
VERSION     DASUGB010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165609"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VFV87_09095"
     CDS             <1..1776
                     /locus_tag="VFV87_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103952.1"
                     /translation="RTEGSLIAAYAHDVLDADHLSVVAGNTDYGKSLAASIVAGFRPF
                     GAVRNLLEVDMGDGREKETTLAAVDKLKADPDPGLIVLALLTDPAAQALRALKAAGVT
                     APIIGGDAVASDALLATIADSGGQAPAWGGLYAAAPLMMDSLTSDALRFHDAYRQAFG
                     LAPTWRSAATYDAAIAATTAMRAAGVTGAATDRAEERQRIRDALAAMDSPDRAVPGLL
                     GPIYFDSHQTTPRAAVFAVARGSKFDSAYEQLRPYGGSGSTDLAEDLASGAAVQVNGQ
                     TLERQRVVFAGIELNEIGELDTTNPSFYADFFLWLTYTGDDTATDIVFMNAADSTLQL
                     GQPERSDVRDGINYRLYHVTGRFKAPLEFHDFPFDRQHLTISFRNRLLPSSELVYALD
                     HHFRDESQEERLQSGANAAVSINTIPNWQASEVELYQGSLGSTEILGDLDADPSTGGL
                     EYSQFTIDVTIERNLAAFLVKNLLPLALLAMITYVSLFFPFKQATTRVSFGISGILTA
                     AVLLSSVTSALPQVGYTVAIEWGFYAFIFLSATCILIGLIGDRLYEQRRFSDLRRLDI
                     FARIYYPAFVLVVVLAYFLHFSDRI"
     gene            2317..2655
                     /locus_tag="VFV87_09100"
     CDS             2317..2655
                     /locus_tag="VFV87_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103953.1"
                     /translation="MPDGTLDNSGCDGDTPCCHRLVQDCPDQKIFCFTSDEHQSQCFD
                     FQGAIGPKGMCWSFGFGCCPCENETQEYWSDQCTKTFFGCGGTCVVGNEFPWECLSFR
                     NCSTGPEGRS"
BASE COUNT          476 a          861 c          814 g          537 t
ORIGIN      
        1 cgcacggaag ggtcattgat tgcggcctat gcccacgacg tcttggacgc cgatcacctg
       61 agcgtcgtcg ccggcaatac cgactatggc aaatcgctcg ccgccagcat cgttgcggga
      121 tttcggccgt tcggcgcggt gcgcaacctg ctcgaggtcg acatgggtga tgggcgggag
      181 aaggaaacga ctctggcggc tgtggacaag ttgaaggcgg atcctgaccc tgggctcatc
      241 gtcctcgccc tcctgacaga cccggccgcc caggccttgc gggcgctgaa ggcggcaggg
      301 gtcacggcgc cgattatcgg cggtgatgcc gtggcttccg atgcgctctt ggccacgatc
      361 gcggattcgg gcgggcaggc gcctgcctgg ggcggactct atgcagcagc gccgctgatg
      421 atggacagcc tgaccagcga cgccctgcgc tttcacgatg cgtaccggca agcctttgga
      481 ttggcgccaa cctggcgcag cgccgcgacg tatgacgccg cgatcgccgc cacaacggcc
      541 atgcgtgcgg ccggcgttac cggtgccgca actgaccgtg ccgaggagcg gcagcgcatc
      601 cgcgatgcgc tggccgccat ggatagtcca gaccgtgcgg tgcccggctt gctcggcccg
      661 atctacttcg acagccacca gacgacaccc cgtgccgcgg tcttcgccgt tgcacggggg
      721 tcgaaattcg actcggccta tgagcagttg cgcccctacg gcggttcggg gtcaacggac
      781 ctggcggagg atctagcaag cggcgcggcg gttcaggtca acggccagac cttggagcgg
      841 cagcgggtgg tgtttgccgg catcgagctg aatgagatcg gcgagcttga cacaaccaat
      901 ccgagcttct atgccgattt cttcctctgg ctcacctata caggcgatga tacggcaacg
      961 gatattgtgt tcatgaatgc ggctgattca acgttgcagc ttggccagcc agaacgatcg
     1021 gatgtgcgag atggcataaa ctacagactg tatcacgtca ccggccgatt taaagctccc
     1081 ctggaattcc acgattttcc gtttgaccga cagcacttga ctatcagttt ccggaatcga
     1141 cttttgcctt cctcggaact cgtctatgcc ctcgatcacc acttccgcga tgaatcgcag
     1201 gaggagcgct tgcagagtgg cgccaacgcg gccgtctcaa tcaacacgat cccgaattgg
     1261 caggcgagtg aagtggaact ctatcagggt tcgctcggtt cgacggagat cctaggagat
     1321 ctcgacgcgg acccgagcac tggcggtctc gagtattcgc aattcacgat cgacgtcacc
     1381 attgaacgaa acttggccgc gtttctcgtg aagaaccttt tgccgcttgc gctcttagcg
     1441 atgatcacgt acgtgtccct cttcttccca ttcaagcagg ccacgacacg cgtgtcgttt
     1501 ggcatcagcg gcatcctgac ggctgcggtg ctcctctcct cggtgaccag cgcgctgccc
     1561 caagttggat acactgtggc catcgagtgg ggcttttacg cgttcatctt cctctccgca
     1621 acctgcatcc tgattggact tatcggagat cgcctctatg agcagcgccg atttagcgat
     1681 ttgcgacgcc tcgatatttt cgctcgcatc tactatcccg cgttcgtcct cgtcgttgtg
     1741 ctcgcatatt tccttcattt cagcgatcgt atctgaacct catgcgaacc aggatcgcag
     1801 ctacggacct tgtccgcccg cggttgccga gcggtcgcgc accatgctta tcgcggcgct
     1861 cgttcgcaca cgaactcggg aggacccgcc catggaccac gagcgcttcg ataccctggc
     1921 ccgtcgcgtc ttcaccgatc tcaagcagtc ccgccgcgcc gcggtggcca cgctcctcgc
     1981 taccaccctg ctgcggcaca cgcccagcac ggccctcgcc aagacgaaga aggcccaggc
     2041 caccgccgcc gatccgtgtt gtcccggggg aaagacgtgc agcccgggca agggcaagat
     2101 cacctccggg tgcaatttca ccttctcgac ggtgttccgc aatcgggacg cccatggcgc
     2161 caatctcagc cacagcagct tcgacaacgc tgacctgagc ggcgccgatt tccggggtcc
     2221 gacctggccg acggctgctt catcaacgcc aacctcaccg gggccaagtt gggcaggtcg
     2281 gtcaacctgc gtggggccgt cttctatcgc acgaccatgc ccgatggcac gctcgacaac
     2341 agcggctgcg acggcgacac gccctgttgt caccggctgg tgcaggactg ccccgaccag
     2401 aagatcttct gcttcaccag tgacgaacac cagtcgcagt gcttcgactt ccagggtgcg
     2461 atcgggccga aggggatgtg ctggagtttc ggtttcgggt gctgtccctg cgaaaacgag
     2521 acgcaggagt actggtctga ccaatgcacc aagacctttt tcggttgcgg ggggacctgc
     2581 gtggtgggca acgagttccc ctgggagtgc ctgtcgtttc gcaactgctc gacgggccct
     2641 gagggacgct cgtagttccg ggggcgctcg gggacgctga ccgagctg
//
LOCUS       DASUGB010000473         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1165653, whole genome shotgun sequence.
ACCESSION   DASUGB010000473 DASUGB010000000
VERSION     DASUGB010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165653"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..871)
                     /locus_tag="VFV87_09105"
     CDS             complement(92..871)
                     /locus_tag="VFV87_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEX5103954.1"
                     /translation="MSEFASGSAPGFVAEVEAAETNVLWVGRHGQDLAATRKITLLAS
                     SIDSDNSPTSTLRGGNVLAIIDSSGKATLYDPDATDGTQIAVGILEKPQSMLVDGTAT
                     DRFTQMLVHGLVKEGELLGLDPRGKQQLGLRCTFDRETSPQAGVLMHPRGIYRKSTNY
                     TVVAGDNGLLFLATAAATFTLPTKENGLAFRFAQTADANLVIQGSADLIHKGNAAANT
                     VTFSTASEKIGSQVLVECLYTNTGTLKWLVTNLGATTPTVA"
     gene            complement(1013..1660)
                     /locus_tag="VFV87_09110"
     CDS             complement(1013..1660)
                     /locus_tag="VFV87_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103955.1"
                     /translation="MSDRGALASAPAAESPETKQTDLAHELLSAASNAPDRREVAGGA
                     KSAETNVVAPSLGGGEPIAEAAAADTEPQSEDEASPEEARPVDAGAEQGRPDQVRPAQ
                     APPTDASADAARQRLAERIAASPTLPPGLRTRLAEVAAAGGGLEQAVLAVEQGLPGVL
                     RVAHREASRTEHPAGEVFFHGDAGAISDQQAETIAQGQLARSGLLRGQRVRVAAD"
     gene            1880..2908
                     /locus_tag="VFV87_09115"
     CDS             1880..2908
                     /locus_tag="VFV87_09115"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5103956.1"
                     /translation="MAIVETRRVFVGLDYHQEKVQVCVVDDQGKLLLDKPAGNAWGVI
                     AAAANRYGQVQGVAIECCCGAANLAKELADKAGWNVSLAHPGFVARMKQNPDKTDFTD
                     ARILADLHRVGYLPKVWLAPNEIRELRRIVRYRQELVDERRNVKLRIRALLRDERIRL
                     PEECGRPWTRAWLAGLRGIDQLAATSRWILDEQLERLTSLNGRIREVESRLEDLVAED
                     AVVKKLRTFQGIGLITATVMRAEIAQFDRFQTGKQLSKFCGVTPRNASSGNRQADAGL
                     LKAGNKQLRTVVIEAAHRLMRCDDRWLAMACRLKQAGKPTCVVIAAVANRWIRWLHHQ
                     MQPELMAA"
     gene            3269..>4200
                     /locus_tag="VFV87_09120"
     CDS             3269..>4200
                     /locus_tag="VFV87_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5103957.1"
                     /translation="MKIAAIECHPVRIPLKPAYRMISALGRHWESQFVLVRLLTDDGL
                     EGAGEASATVRWSGETVWGVQAVIERVFTPLLVGRELDPQSPLETIAAIDRLLDQAAA
                     HNWFAKSALEMACWDVLGKAAGKPVYDLLGGACRPLAIRSRFSMGAYDVPRARARAAE
                     LVEQGFDTVKVKVGGPLEGDVARVRAVRDVIGPARALVIDANCGWDADTAIAAVRELR
                     DCRLAVLEQPTPDGDYEQMARVRRAAGTTGGGVKVMADDMCFNLVHAQELVHNEACDL
                     ISVYPGKNGGIRKSRAIAEFAAGHDIGCTIGSNL"
BASE COUNT          708 a         1343 c         1414 g          735 t
ORIGIN      
        1 caacgagagg gggagggggt gagtggggga agtgcttgtg ctggctaact ccccctctct
       61 ctccctcacc cactccggcg gagccattga cctacgccac cgtcggcgtc gtcgccccca
      121 aattcgtcac cagccacttg agcgtgccgg tgttcgtgta caggcactcg acgagcacct
      181 ggctgccgat cttttcgctg gcggtgctga acgtgacggt gtttgcggcg gcgttgccct
      241 tgtggatcag gtcggccgag ccctggatga cgaggttcgc atcggccgtt tgcgcgaagc
      301 ggaacgccag gccgttctcc ttcgtgggaa gggtgaacgt ggccgccgcg gtcgcgagga
      361 acaacaggcc gttgtctccc gccaccaccg tgtagttggt gctcttgcga tagatgcccc
      421 gcgggtgcat gaggacgccc gcctgcggcg aggtttcgcg gtcgaaggta caccgcaggc
      481 caagctgctg cttgccgcgc gggtccagcc ccaagagctc gccttctttc accaggccgt
      541 ggacgagcat ctgcgtgaag cggtcggtgg ccgtgccatc gaccagcatg ctttggggct
      601 tttcgaggat gccgacggcg atttgcgtgc cgtcggtggc gtcgggatcg tagagggtcg
      661 ccttgccgct ggaatcgatg atcgccagga cgttgccgcc gcgcagcgtg gaggtggggg
      721 aattgtcgct gtcgatactt gaggccagca gcgtgatttt gcgggtggcg gccaggtcct
      781 gcccgtgccg cccgacccag aggacgttcg tctcagcggc ctcgacttca gccacgaagc
      841 ccggcgcgct acccgatgcg aattccgaca tgagatgctc cttgaaagga agtgaaggtg
      901 tgaaggggag tgaagtgaat gaaagaaatg gtttgaagtg gtacgtggtc cgcggtcagc
      961 agtcagtgaa tctctctgtc tctccgtctc tctgtctcgc cgacctccga cctcagtccg
     1021 ccgccacgcg cacccgctgg ccgcgcagca ggcccgatcg cgcgagctgt ccctgggcga
     1081 tcgtttcggc ctgctggtcc gaaatggccc cggcatcgcc gtggaagaag acctcgccgg
     1141 caggatgctc cgttcgggac gcctcgcggt gtgccacccg cagcactccg ggcaaacctt
     1201 gttcgacagc cagcactgcc tgttcgaggc ccccacctgc ggcggcgact tccgccagtc
     1261 gcgttcgcag tcccggcggc aatgtcgggg aggcagcaat tcgctcggca agcctttggc
     1321 gggcggcatc ggcgctggcg tcggtggggg gcgcctgtgc cggacgcacc tgatccgggc
     1381 gtccttgttc ggcgcccgca tctaccgggc gcgcttcttc tgggctcgct tcgtcctcgc
     1441 tctgcggctc tgtgtcggca gctgcggcct ccgcgattgg ctcgccgccg cccagggacg
     1501 gcgcaacgac gtttgtctcc gcgctctttg cgccgccggc aacttcgcgc cgatccgggg
     1561 cattggacgc tgccgacagc aactcgtgcg cgaggtccgt ttgcttcgtt tctggggatt
     1621 ccgccgccgg cgcggatgca agcgcaccgc gatcgctcat gggacacctc ccaaggttgg
     1681 ggaaagatcg aaaaccggcc acgcgcctca ggcggccgtc acctgccgaa cggcgctccg
     1741 cgccgctcgt gcgcccgcag gccgcgagcg cgagaatggg aggctgaatt acctcagatg
     1801 gggatgaaag cccgttgctc cttgaaggct ggtagaaaac ggttggtcga actgtttccc
     1861 cagtcaagga gcaacgagca tggctattgt agaaactcgg cgggtctttg tgggtctgga
     1921 ctatcatcag gagaaggtcc aggtgtgtgt cgtggacgac caagggaagc tgctattgga
     1981 caagcctgcg ggcaacgcct ggggtgtgat tgcggcagcg gccaatcgct acggccaagt
     2041 ccaaggcgtg gccatcgagt gttgctgcgg agcggcgaat ctggccaagg agttggccga
     2101 caaggctggc tggaacgtga gcctggccca ccccggcttc gtcgctcgga tgaagcagaa
     2161 ccccgacaag accgatttta cggacgcccg catcctggcc gacctccacc gcgtgggcta
     2221 ccttcccaaa gtgtggctgg ctcccaacga gattcgcgag ctgcggcgaa tcgttcgcta
     2281 tcggcaagaa ctggtggacg agcggcgcaa cgtcaagctc cgcattcggg ccttgctccg
     2341 cgatgagcgg attcgtctgc ccgaggagtg cggccgtccc tggacccggg cctggctcgc
     2401 cggacttcgc ggcattgacc aactggcggc caccagccgc tggatcttgg acgaacaact
     2461 agagcggctg acgagcctga atgggcgaat ccgcgaggtc gagagccggc tggaagacct
     2521 ggtggccgag gatgcggtcg tgaaaaagct gcggaccttt cagggaatcg gcctcatcac
     2581 ggcaaccgtc atgcgggcgg agatcgccca gttcgaccgc tttcaaacgg gcaagcagtt
     2641 gtccaagttc tgcggagtga cgccgcggaa tgcctccagc ggcaaccgcc aggccgacgc
     2701 cgggctactc aaggcgggga acaaacaact gcggacggtg gtcatcgagg cggctcaccg
     2761 cctgatgcgc tgcgacgatc gctggctggc gatggcctgc cgcttgaagc aggctggcaa
     2821 gccgacctgc gtcgtgatcg cggcagtggc caaccgctgg atccgctggc ttcatcacca
     2881 gatgcaaccg gaattgatgg ccgcctaaag ggacgaccgg gaacgaatca acgcgggaga
     2941 aaacttcaaa accagacctt ccaagagagg aagacacttc accgtggcct gagtaggtgc
     3001 cggcgagctc gagggtggct tgggctatca gctcgtccac agatggggca gccggcgccg
     3061 cttcgaaaca acccctatgg gcaagggcct tggcccgacg ctcggataga agattgggca
     3121 cacacggcga attgcactcc acctcttgcg gagcaatgga ctttcataga agccacccca
     3181 tccggatctc cgccgcattc tcgcggactc tctgcgcttg tacgatgcgt ttcccgctgc
     3241 gcgaggcagc ccgccgcgag aaacgcccat gaaaatcgcc gctatcgagt gccaccccgt
     3301 tcgcatcccg ctcaagcccg cctaccggat gatctcggcc ctcggaaggc actgggaatc
     3361 gcagttcgtc ctggtgcggc tcttgaccga cgacggcctg gaaggagccg gcgaagcctc
     3421 ggccaccgtc cgctggagcg gcgaaacggt gtggggcgtg caggcggtga tcgagcgggt
     3481 gtttaccccg ctgctcgttg gccgcgagct cgacccccag tcgccgctgg aaaccatcgc
     3541 cgctatcgac cggctgctcg accaggccgc ggcccacaat tggttcgcca agtcggccct
     3601 cgaaatggcc tgctgggacg tgctgggcaa ggcggccggc aagccggtct atgacctgct
     3661 cggcggggcc tgccgccccc tggccatccg cagccgcttt tcgatgggcg cctacgacgt
     3721 gccccgagcc cgcgcccggg ctgcggaact ggtcgagcag ggctttgaca ccgtcaaagt
     3781 gaaagtcggc ggcccgctgg agggcgacgt ggcccgcgtc cgcgcggtgc gcgatgtcat
     3841 cggcccggcg cgggccctcg tgatcgacgc caattgcggc tgggacgccg acacggcgat
     3901 cgctgccgtc cgggagctgc gcgattgccg cctggccgtc ctggagcagc cgacacccga
     3961 cggcgactat gagcagatgg cccgtgtgcg tcgagccgct ggcacgactg gcggcggcgt
     4021 gaaggtaatg gccgacgata tgtgcttcaa cctggtccac gctcaggagc tcgtgcacaa
     4081 cgaggcctgc gatctgatca gcgtctatcc gggcaaaaac ggcggcatcc gcaagtcgcg
     4141 ggcgattgcc gaatttgcgg ccggccacga catcggctgc acgatcggct cgaacctgga
//
LOCUS       DASUGB010000474         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_53742,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000474 DASUGB010000000
VERSION     DASUGB010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53742"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFV87_09125"
     CDS             <1..856
                     /locus_tag="VFV87_09125"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5103958.1"
                     /translation="VSEYRIGDLVVPAVRRAKPGQTRRLDYLPLGAFTERGIWSEHGF
                     SQPLWLDRPEHLYRAGAGIEDLAVFAEPLAVAEKGVNEAHTLQRARLGEACWTKMPPR
                     VLVTGMGPIGFAAVIACVARGWPVTMYGRDPDDSFRARLVQEFGAEYVPQERINFDQP
                     DVEATGVDLVLECTGSDEVMLATSGLLRSCGILVWLGSTRLPRPKPHNVEKLMRDGLI
                     RNHLHVGCVNAAPRDFRDALAHLAQLKSRFGSQLAALITARVRLEDSLWHYEHRQPQG
                     IKTVIEYE"
     gene            1019..1612
                     /locus_tag="VFV87_09130"
     CDS             1019..1612
                     /locus_tag="VFV87_09130"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5103959.1"
                     /translation="MSNVVTMPWEILDRMERAVAKVRDRLLRATAALNQAGVPYAVVG
                     GHAVASWVATVDEGAVRNTRDVDLLVRREDLTAVTTALQKAGFTPEQSFGVTMFLDGP
                     DAKPSESIHLLFEGEKVKENDPFDAPTITTTDDPAGFHVLTFESLVRMKLLANRDKDK
                     THIRDMIGVGLIDQTWLAKLPPVLAERLKAILDTPEG"
     gene            complement(1658..1927)
                     /locus_tag="VFV87_09135"
     CDS             complement(1658..1927)
                     /locus_tag="VFV87_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103960.1"
                     /translation="MVRLKFLTLILVLAAGTVVPSVATAGPPSYLLLRDPESPGPKLQ
                     PGYAASKSYDVRSYGYAYGYFGVAPRTHCSHHFGIYRLYTQWTRW"
     gene            complement(2130..3161)
                     /locus_tag="VFV87_09140"
     CDS             complement(2130..3161)
                     /locus_tag="VFV87_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5103961.1"
                     /translation="MNGDKDSGRAVLPPGQQLAAPGKWPLVGERLPAESAEPCSVEIG
                     GLVERPVRLSLEDLQRLPQVEAEVDIHCVTRWSQPGMRFGGVTLQALLELAGCQPGAR
                     FVSFVARSSRQHSTSLVLEDAVQLGTLVALSAGGQPLPTLHGGPVRTVVPGRYFYKSL
                     KWLTRIELLAEDRLGYWEAVAGYHNHADPWQEQRFIASSISRHQAAELIASHVFRGDL
                     RGIDCRGRDLANLQAAGALLRDADFREAALQGADFNGANLSISKFQQADLRQASFRDA
                     DCEGCDFSGADLRGADFRGATLFGATLVDHAAGLAAKIDASTRFDRERLGDLTPVQLD
                     FILQSPAVC"
     gene            3231..3905
                     /locus_tag="VFV87_09145"
     CDS             3231..3905
                     /locus_tag="VFV87_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5103962.1"
                     /translation="MSPAIAYSFGIVAYMALFAAVGIAFLFVNLLVGKFLRPHDPHAE
                     KLEIYECGEPTIGSSFVQFDLRFYVVALLFIIFDVEVAMFFPWAAAFGKSVNMADANF
                     EVASRDPATNAVVLTSPARELYQELGVRNPTVPETVPPSLADQVTASGDIETAQAVIH
                     AGASQIAWITLIDVAAFFAVLLVGFAYVWSRGDLDWVRAMSERHRAEPRPFSDDPLEQ
                     EAALSA"
     gene            3948..4475
                     /locus_tag="VFV87_09150"
     CDS             3948..4475
                     /locus_tag="VFV87_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5103963.1"
                     /translation="MAAPLLDRLKKKFGDRISGANMEAIDPWIEATPAGLVEVCQYLK
                     SEPDLSFELLNCITGVDYFEPDPKKAAKATFQPHTEVVYHLSSVTKKHSLVLKVILPR
                     WKNDQPGELPEVPSISSVWPGANWHEREVFDLSGVKFVGHPDLRRILCPEDWEGYPLR
                     KDYEMPLEYHGMRGR"
     gene            4491..>4696
                     /locus_tag="VFV87_09155"
     CDS             4491..>4696
                     /locus_tag="VFV87_09155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911371.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5103964.1"
                     /translation="MATALDDPRIVEFDVRTDEMLVNMGPQHPSTHGVLRLVLRTDGE
                     VVSQVEPHIGYLHRCAEKIGENLTP"
BASE COUNT          866 a         1528 c         1509 g          793 t
ORIGIN      
        1 cgtgagcgaa tacaggatcg gcgacctggt ggtgcctgcc gttcgccggg ccaagcccgg
       61 gcagacgcgg cggctcgatt acttgccgct cggcgcattc accgaacggg gcatctggtc
      121 ggagcacggt ttcagccagc cgctgtggct cgaccgaccc gagcacctgt accgcgcggg
      181 ggccggaatc gaagacctgg ctgtgtttgc cgagccgctg gcggtcgcgg aaaagggcgt
      241 caacgaagct cacacgctgc agcgagcgcg gctgggtgag gcgtgctgga cgaaaatgcc
      301 gccgcgggtg ctcgtcaccg gcatgggacc gatcggcttc gccgcggtga tcgcctgcgt
      361 ggctcgcggc tggccagtga cgatgtatgg ccgcgatccg gacgattcat tccgcgcgcg
      421 gctcgtgcag gagttcggag cggagtacgt gccgcaggag cgtatcaact tcgaccagcc
      481 ggacgtggaa gcgaccggcg tcgatctggt cctggaatgc accggcagcg atgaagtgat
      541 gctggcgacg agcgggctgc tccgctcttg cggcatcctg gtctggctcg gcagcacgcg
      601 ccttccgcgg ccgaagccgc acaacgtcga aaagctgatg cgcgacggcc tgatccgcaa
      661 tcacctgcac gtcggctgcg ttaacgccgc gccccgcgac ttccgcgatg ccctcgccca
      721 cctcgcccaa ctcaagtcgc gattcggcag ccagctcgcc gccctgatca ccgcccgcgt
      781 tcgactcgaa gattccctct ggcactacga gcaccgccag ccgcagggga tcaagacggt
      841 gatcgagtac gagtagggtg ggaccagcga gcgaagcgag cgcaggtcca ccagagcgcg
      901 gcttgcggcg aaatcacgga gggcctgcgg atcatggtgg gccggcggcc gcggcggcct
      961 tgtcccaccc tacaaattgc cgtggcaacc gcccccaggc aaagctacga ttgaagagat
     1021 gtccaacgtc gtcacgatgc cctgggaaat tctcgaccgc atggagcgag ccgtggcaaa
     1081 agtgcgcgat cgtttgctgc gggcgaccgc ggcgctcaat caagccggtg ttccgtatgc
     1141 ggtggtcggc gggcacgcgg tcgcgtcgtg ggtcgcgacg gtcgatgaag gggccgtgcg
     1201 gaacacgcgt gacgtcgatc tgctcgtccg ccgcgaggat ttgacggcgg tcactacggc
     1261 cctgcagaaa gccggcttca cgccggaaca gtcgttcggc gtgacgatgt tcctcgatgg
     1321 tccggacgcc aagccgagcg agtcgatcca cttgctcttt gaaggagaga aggtcaagga
     1381 gaacgatccc tttgacgcgc ccaccattac gaccacagac gatccggccg gtttccacgt
     1441 gctcacgttc gaatcgctcg tgcgaatgaa gctgctggcg aatcgggaca aggacaagac
     1501 gcacatccgc gacatgattg gcgtcggcct gatcgaccaa acgtggctcg ccaagctgcc
     1561 gccggtcctc gccgagcgac tgaaggcgat tctcgacacg ccggaaggtt aaacgtggtg
     1621 ggcctgcggc cgcggcggcc ttgtcccact ttaccaatca ccaccgtgtc cactgcgtgt
     1681 agaggcggta aatgccgaag tggtgcgagc agtgcgtccg cggggccacg ccgaagtagc
     1741 cgtatgcgta gccatacgag cggacgtcgt aactcttgga ggcggcgtag cccggctgca
     1801 gcttgggtcc gggagattct ggatcgcgca gcagcagata cgaaggcggc ccggccgtgg
     1861 cgacgctcgg caccaccgtg ccggcagcga gaaccagaat cagggtcaag aatttgagtc
     1921 gcaccatacg aaccagatcg acgccgtttc gctccccgcg gcaggcaaag tcagccggat
     1981 cggcaggatc gcatgctggt tctggaaaac tttgcgggca gcgcaatcga ggcaagatgc
     2041 tagcgcctgg cccatactaa cccgaagcgt aagcgagggc gtccctcgct tacgctccgg
     2101 gttggtgtca gctcgggcgc tgcaagccgc tagcacaccg ctggcgactg gaggatgaaa
     2161 tcaagctgca ctggagtcag atcgcctaga cgctcgcggt caaaccgcgt tgaggcgtcg
     2221 attttcgccg ccaggccggc cgcgtgatcg accaacgtcg ctccgaagag cgtggcgccg
     2281 cggaaatcgg ctccccgcaa atcggcgccg ctgaaatcgc agccttcgca gtcggcatcg
     2341 cgaaacgagg cctgccggag gtcggcttgc tggaacttcg agatcgagag attggcgccg
     2401 ttgaagtccg caccttgtag agcggcttca cggaaatcag catcgcgcag tagcgcgccg
     2461 gccgcctgca ggttcgccag atcgcggccg cggcaatcga tcccccgcag atcgccgcga
     2521 aacacgtggc tggcgatcag ctccgcggcc tggtgccgcg agatcgacga ggcgatgaac
     2581 cgctgctctt gccacgggtc ggcgtggttg tggtagccgg cgaccgcttc ccagtagccc
     2641 aggcggtcct cggccaatag ctcgattcgc gtcagccact tcaggctctt gtagaaataa
     2701 cggccaggca cgaccgtgcg gaccggcccg ccgtgcaggg tcggcagcgg ctggccgcca
     2761 gccgagagcg cgaccagcgt gccgagctgc acggcgtctt cgaggacgag cgacgtgctg
     2821 tgctgcctcg agctgcgggc gacaaacgaa acgaaccggg cccccggctg gcagcccgcc
     2881 agctccagca gtgcttgcag agttacgccg ccaaaccgca tccccggctg cgaccaccgc
     2941 gtgacgcagt gaatatcgac ctccgcctcc acctgcggca gccgctgcaa gtcctccagt
     3001 gaaaggcgca ccggccgttc caccagcccg ccgatctcga ccgaacaggg ctccgcggat
     3061 tcggccggca ggcgttcgcc gaccagcggc cactttcccg gtgcggccaa ctgctggccg
     3121 gggggaagaa ccgcccgtcc actgtccttg tcgccgttca tggatcgctt acaatgcccg
     3181 gattgatcgt gaaatagttc acaagccagc cattccggcg aggcccacct atgtcgcccg
     3241 cgattgccta ctcgtttggc attgtcgcct acatggccct gttcgcggca gtcggcatcg
     3301 cgttcctgtt cgtcaacctg ctcgtcggca agttcctgcg gccgcacgac ccgcacgccg
     3361 aaaaactcga aatctacgag tgcggcgagc cgacgatcgg ctccagcttc gtgcagttcg
     3421 acttgcgatt ttacgtcgtc gccctgttgt tcatcatctt cgacgtggaa gtggcgatgt
     3481 tcttcccgtg ggcggcggcc ttcggcaaat cggtcaacat ggccgacgcg aatttcgagg
     3541 tggccagccg cgatccggcc accaacgccg tcgtgctcac gtcccctgcc cgcgaattgt
     3601 atcaggagct gggcgtccgc aatccgacgg tgcccgagac cgtgccgccg tcgctggccg
     3661 accaggtcac cgccagcggc gacatcgaaa cggctcaggc ggtgattcac gccggagcga
     3721 gccagatcgc ctggatcacg ctgatcgacg tggccgcgtt ctttgccgtg ctgctcgtcg
     3781 gctttgcgta cgtctggagc cgcggcgacc tcgactgggt ccgcgcgatg agcgagcggc
     3841 accgcgccga gccgcgaccc ttcagcgacg acccgctgga gcaggaagca gcgctgtccg
     3901 catagcggaa actcgacatt tgaccttcgc ccttcgacat tccccccatg gccgctccgc
     3961 tcctcgaccg gttgaagaag aagttcggcg acaggatctc cggcgccaac atggaggcga
     4021 tcgatccctg gatcgaggcg acgccggccg gcctcgtcga ggtttgccaa tatctcaagt
     4081 ccgagccgga cctgagcttc gagttgctca actgcatcac cggcgtcgat tatttcgagc
     4141 ccgatccgaa gaaggccgcc aaggccacct tccagcccca cacggaggtc gtctatcacc
     4201 tctcgagcgt gacgaagaag cactcgctgg tgctcaaggt cattctcccc cgctggaaga
     4261 acgaccagcc gggcgagctc cccgaagttc cctccatcag cagcgtctgg cccggcgcca
     4321 actggcacga gcgggaagta tttgatcttt cgggtgtaaa attcgtagga cacccagatc
     4381 ttcgaaggat cctttgtccg gaggattggg aagggtatcc cttgcgaaaa gactacgaga
     4441 tgccgctgga ataccacggc atgcggggcc gctgaccaac gaaactaact atggctactg
     4501 ctctcgatga tccgcggata gtggagtttg acgtccgaac ggacgaaatg ctcgtcaaca
     4561 tgggacccca gcaccccagc acgcatgggg tgctgcggct tgtgctgcgg actgacggcg
     4621 aagtcgtttc gcaggtcgag ccgcacattg gctatctgca ccgctgtgcg gaaaagatcg
     4681 gcgagaacct cacgcc
//
LOCUS       DASUGB010000475         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_53974,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000475 DASUGB010000000
VERSION     DASUGB010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53974"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFV87_09160"
     CDS             <1..613
                     /locus_tag="VFV87_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5103965.1"
                     /translation="KKLTVEQNLLGVMELLNMPRRERRRRCEELLEQFQIAHIKKSRG
                     GSLSGGERRRLEIARCLVSEPEIILLDEPFTGIDPVTIESIQGIIRDLKARGISILIT
                     DHQVRETLQITDRSYVIHKGQVLCDGPPDVVLRNPKARELYFGSNLDVDRPALTIPPP
                     PAPPPPKSSARVDEAAQQPPAPFTDKRSAKRSVHEDETLRDDS"
     gene            747..>2057
                     /locus_tag="VFV87_09165"
     CDS             747..>2057
                     /locus_tag="VFV87_09165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX5103966.1"
                     /translation="MNLTAPATSSLAAARVILRPQKALPFYGRHPWVLESAVERVEPT
                     SIAGEHLLEIDGAVVDLVNDRGKFIARGFYNSQSRIRVRLLTWNAEEALDEAFFRRRI
                     EAAIELRRRIGYPGSGVDSRTESGDANNLSRESTPAPFDVARPIGVTRPADPSPPPPL
                     PQGERGERNATEGVPYSESAARLVFSEADGLSGLVVDRYGDYLALQPTSLAMAQRLEM
                     IVAILHDLLQPRAVVLRAEKSMAQLEGVEIAEGHVWGELPEGPITIREHGLVYEFDLH
                     VGQKTGFYLDQRENRRVAATYVKDRRVLDMFCYTGGFAMAASALGGSREVLAIDSSKK
                     AIALAQRNAELNGLKNVTFEVGDGFQTLDALLARGERFEAVILDPPKFARGKSGVNKA
                     LMAYHRLNRAAVELLVPGGILITCSCTGSVSREDFLLMLSGVAQK"
BASE COUNT          378 a          670 c          669 g          340 t
ORIGIN      
        1 caagaagctg accgtcgagc agaacctgct gggtgtcatg gaattgctca atatgccccg
       61 ccgcgagcgt cgccgccgct gcgaagagct gcttgaacag ttccagatcg cgcacatcaa
      121 gaaaagccgc ggtggctccc tctccggcgg cgagcggcgg cgactggaga tcgccagatg
      181 cctggtcagc gagccggaga ttatcctgct cgacgagccc ttcaccggaa tcgacccggt
      241 gacgattgag agcatccagg ggatcatccg cgacctgaag gcccgcggca tctcgatcct
      301 gatcaccgac caccaggtgc gcgagacgct gcagatcacc gaccgcagct acgtgatcca
      361 caaggggcaa gtcctctgcg acggcccgcc cgacgtcgtc ctccgcaacc ccaaggcccg
      421 ggagctctac ttcggcagca acctcgacgt cgatcggccg gcgctgacca ttccgccgcc
      481 gcccgctccc ccgcctccta aaagcagcgc ccgcgttgat gaagccgccc agcagcctcc
      541 cgcccccttt accgataagc ggtcggctaa acgatcggtt cacgaggacg aaacgctacg
      601 ggacgattcg taggcatcct cgctccgcgg caggtggcta ctctcgcagc gagtagccag
      661 cgatcttggc ccgccgcacg cggacgggag agcagatatg atgagggatc gccacttcag
      721 tatccgcctg cgatccactg tccacgatga accttactgc tcctgcaact tcgtcgctgg
      781 ccgctgcccg cgtcatcctt cggccgcaaa aggcgctgcc cttttatggg cggcatccgt
      841 gggtgttgga gtcggccgtc gagcgggtcg agccgacgag catcgccggc gagcacttgc
      901 tggaaatcga cggggccgtc gtcgatctgg tcaatgaccg cggcaagttc atcgcccgcg
      961 ggttctacaa cagccagagc cggattcgcg tgcggctgct gacgtggaac gcggaggagg
     1021 cgctggacga ggcgttcttc cggcggagaa tcgaggcagc gatcgagttg cggcggcgga
     1081 ttgggtatcc ggggtcggga gtcgattcgc gcacggagtc tggcgatgca aacaatctca
     1141 gccgcgaatc gactcccgcc ccctttgacg tcgcccggcc aattggcgtt acccggcccg
     1201 ctgacccctc acccccgccc cctctccccc aaggggagag gggagagcgg aacgccacag
     1261 agggcgttcc ctacagcgaa tccgccgcgc gcctcgtctt cagcgaagcc gacggactca
     1321 gcgggctggt cgtcgatcgc tacggcgatt atctcgccct tcagccgacg tcgctggcga
     1381 tggctcagcg gctggagatg atcgtggcga tcctgcacga cttgctccag ccgcgggcgg
     1441 ttgtactgcg ggccgagaag agcatggcgc agctcgaagg cgtcgagatc gccgaagggc
     1501 acgtgtgggg agaactgccg gaagggccga ttacgatccg cgagcacggc ctggtatacg
     1561 aattcgatct gcacgtgggg cagaagaccg gcttctatct cgaccagcgc gagaaccgcc
     1621 gtgtagccgc gacttacgtc aaagatcgcc gcgtgctcga catgttctgc tacacgggtg
     1681 ggttcgcgat ggctgcgtcg gcgctcggcg ggtcgcgcga ggtgctcgcc atcgacagca
     1741 gcaagaaggc aatcgccctg gcgcagcgga acgcggaact gaacgggctg aagaacgtta
     1801 ccttcgaggt gggggatggc tttcagacgc tggacgccct gctcgctcgc ggcgagcggt
     1861 ttgaggcggt catcctcgac ccgccgaaat tcgcccgcgg caaaagcggc gtgaacaagg
     1921 ccctgatggc gtatcatcgg ctcaaccggg cggcggtgga attgctcgtt cccggaggaa
     1981 tcctgatcac gtgcagttgc accggcagcg tcagccgcga agacttcctg ctgatgctct
     2041 cgggcgtggc gcaaaag
//
LOCUS       DASUGB010000476         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2000124, whole genome shotgun sequence.
ACCESSION   DASUGB010000476 DASUGB010000000
VERSION     DASUGB010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000124"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFV87_09170"
     CDS             complement(<1..341)
                     /locus_tag="VFV87_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103967.1"
                     /translation="MNLARASRIAEWALVVAVGLLHVSIFFWAERADQPSFLVSTTHD
                     AQLYSAVALILAWTVLGPGWVWLRGGALPLLVIAWFLPWNTQMFPRETAASFPLAVAA
                     SAALIVVAIRV"
     gene            539..1276
                     /locus_tag="VFV87_09175"
     CDS             539..1276
                     /locus_tag="VFV87_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5103968.1"
                     /translation="MFKIKICGITSVQDARLAAEAGADAIGLNFYAQSPRHVAADLAG
                     EIARRLREDYSPDQVQIVAVFVNHAPDEILWTLREADLYASGLCLQLHGDEPPALLHE
                     LQAHGLGTAGHLLQATGHVPAVPVIRALRCPGSELAAATHYLEECKRQSASPQGVLID
                     AFQPGAYGGTGARADWAAVRRRSWSLPLILAGGLTPDNVTEAIATARPDAVDVASGVE
                     SAPGQKDLAKVRAFVAGAKEAFAALDR"
     gene            1783..>2697
                     /locus_tag="VFV87_09180"
     CDS             1783..>2697
                     /locus_tag="VFV87_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5103969.1"
                     /translation="MSTITEKRLSFKVKDLSLAELGRKKIMLAENEMPGLMALRNKYG
                     QSKPLTGARIAGCLHMTIETAVLIETLDFLGAQVTWSSCNIFSTQDEAAAAMAARNIP
                     VYAWKGMTAEEFDWCIEQTLHFPDGQPLNLILDDGGDLTNMVLDKYPELVGGIKGLSE
                     ETTTGVHRLAQRLEAGTLKLPAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAG
                     KVAVVAGYGDVGKGCAHSLRSYGARVLVTEIDPINALQAAMEGFEVTTMEEAASQGNL
                     FVTTTGCCDIIKPEHIQQMKNDAIICNIG"
BASE COUNT          566 a          831 c          830 g          470 t
ORIGIN      
        1 caaacgcgaa tcgcaacgac gatcagcgcc gcactcgccg ccaccgccag cggaaacgaa
       61 gcagccgtct cccgcggaaa catctgcgtg ttccacggca ggaaccaggc aatcaccagc
      121 agcggcagcg ccccgccgcg gagccagacc cagcccggcc ccaggacggt ccaggccaga
      181 atcagcgcga ccgccgagta gagttgggca tcgtgcgtgg tggaaacgag aaagctcggc
      241 tgatcggccc gttcggccca gaagaaaatg ctgacgtgca gcaggccgac cgcgacgacg
      301 agcgcccatt cggcaattcg gctggccctg gcaaggttca tcggcacgtc cctcggttca
      361 caggttagga cgaaccacag cgggcgtcgg ttctaggcgt gaaacaatgg ccgcccttct
      421 ctataatcca gcgtgcccag cgatttattc ccgccaggcc ggcgatttcg ttcccggact
      481 cctgtccgta accgcaataa ctcgcatccc tgcctcaact tacggctaca gccagcctgt
      541 gttcaagatc aaaatctgcg gcattacgag cgtccaagac gcccggctgg cagccgaagc
      601 gggcgcggac gccatcgggc tgaatttcta tgcgcagagt ccgcgacacg tcgccgcgga
      661 cctcgcgggt gagatcgccc gccgcttgcg cgaagactac tcgcccgatc aggtgcagat
      721 tgtcgcggtg ttcgtgaacc atgcgccgga cgagattctg tggacgctgc gcgaggcgga
      781 cctgtacgct tccggccttt gcctgcagct tcatggcgac gagccgccgg cgctcctcca
      841 cgaattacag gcccacgggc ttggtacggc aggtcacttg ctccaggcga cagggcatgt
      901 gcccgccgtg ccggtgattc gtgcgctgcg ctgtccaggc tccgagctgg ctgctgcaac
      961 ccactacctc gaagagtgca agcgtcagtc tgcaagtccg cagggcgtat taatcgacgc
     1021 cttccagcca ggtgcttatg gcggtaccgg agctcgcgcg gattgggcgg ctgtgcggcg
     1081 ccgttcatgg agtctgcctc tcatcctcgc cggtggcctg acgccggata acgtgaccga
     1141 agcgatcgcg acagcccggc ccgatgcagt cgatgtcgcc agcggcgtcg aatcggctcc
     1201 cggccagaag gacctggcca aagtccgggc cttcgtggcg ggggcgaagg aagcatttgc
     1261 ggccctagac agatagtgga ctttaggata gaattcaatt gtttagtaat gcgcgctagc
     1321 gacggccgat tgaactgccg ttcgtatctg ccagattggg actggcggat tgctaccgca
     1381 gggacggcta cgcgggcctt ggcttctcgg cgacagctcg acgaccagac aagatggaag
     1441 acaaatcgaa aaagttgcgg gccattgctt cacaacgcgc gcaactgcgc aacatggagt
     1501 tgcgcgtgga tcaaatggca caacttgtta tacaataagg agttgtgatt gcagttgcgc
     1561 ggccagttgc gcgagaaaaa tcgtcgcgaa gttgcgcgca atggaaatca gggaccaaca
     1621 ggaccgccaa tcgcgatcga ctgttcgtat gcgggagcac tcgtcggccc tacccgcttg
     1681 cgagaaatcc gtttacgatt tctgcggctg aaaaatcaag gaaatgcggc ccctggcagc
     1741 cgtttgggcg tcgctaactg ccccgtcatt caggagcacc aagtgtccac tatcaccgag
     1801 aagcgtctct ccttcaaagt caaagatctc tccctcgccg agcttggccg caagaagatc
     1861 atgctggccg agaatgaaat gccgggtctg atggccctcc gcaacaagta cggccagagc
     1921 aagccgctga ccggggcgcg gattgccggc tgtctgcaca tgacgatcga gacggcggtg
     1981 ctgatcgaga cgctcgattt tctgggggct caggtcacct ggagctcgtg caatattttc
     2041 agcacgcagg acgaagcggc tgccgcgatg gccgcccgca atatccccgt ttatgcctgg
     2101 aaaggcatga ccgccgagga atttgattgg tgcatcgagc agacgctgca cttcccggat
     2161 ggtcagccgc tgaatctgat tctcgacgac ggtggcgacc tgacgaacat ggtcctcgac
     2221 aagtaccccg aattggtcgg cggcatcaag gggctcagcg aagaaacgac caccggcgtg
     2281 catcgtctcg cccagcgcct ggaagccggc acgctcaagc tgccggcaat caacgtcaac
     2341 gactcggtaa ccaagagcaa attcgacaac ctgtacggct gccgggagag cctggccgac
     2401 ggcatcaagc gggcgaccga cgtgatgatc gccggcaagg tggcggtcgt tgccggctat
     2461 ggtgatgtgg gcaagggctg tgcccattcg ctgcggagct acggcgcccg cgtgctcgtg
     2521 accgagatcg acccgatcaa cgccctgcaa gccgcgatgg agggcttcga agtcaccacg
     2581 atggaagaag ccgcctcgca gggcaacctg ttcgtcacga cgaccggctg ctgcgacatc
     2641 atcaagccgg aacacatcca gcagatgaag aacgacgcga ttatctgcaa catcggc
//
LOCUS       DASUGB010000477         4892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_193939, whole genome shotgun sequence.
ACCESSION   DASUGB010000477 DASUGB010000000
VERSION     DASUGB010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193939"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFV87_09185"
     CDS             <1..388
                     /locus_tag="VFV87_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEX5103970.1"
                     /translation="HEDMLATAREVARLRIDAVKIHNLYAVKNTPLADQVERGEVSLM
                     ERDDYIRTLVDFLELLPPKMIVERISGDAPPDYFIGPSWCLDKPAILRALQHELQRRD
                     TWQGRHFPIEPEPSTHKLIVRQPTQA"
     gene            531..2294
                     /locus_tag="VFV87_09190"
     CDS             531..2294
                     /locus_tag="VFV87_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HEX5103971.1"
                     /translation="MGLALPAALRADPVARLSTPAADAVREALQREIYGLETDRRELL
                     AAAAEQAPQYGPARWHLGFVQDGKHGWLQHEAVLQSPKLFQELTAYERQRSKAFDTVA
                     GQLALAEWCREHELADQARAHLTRVIDLNPDHALARAHLGFVRQNGGWVSSAEINRDA
                     TQESARQAALRVWQPRIAAIRKDLEHRSQERREFAQNRLAEIVDPAAVPAIEAVFADA
                     SEDVAVHALDALAKISDPEASLALARFAAYSPGQTVREAAAKRLGKRELESYVPQILA
                     SMYSPLVSRFMAVTLPTGRIGYRHAFVRETHDRQELLLLDTEYRRQALIGGSRGESTS
                     RALDQAADLARQREQAAAAQNEWINRVNDRLAWVLQTATGQNLPAAPEAWWNWWNERN
                     EVFLAGSKPVNTIQQTSQVTIVDSVLIGTSGQLGTSGSSTPTPQTHECLAAGTLVWTV
                     KGPWEIDQVRVGDLVLAQHPETGELAFKPVLRTTVRPKGRLINLQSGGESCQCSGGHP
                     FWVAGEGWVKARELRSGQVLHGAMGPVQISSVEPSIEAETYNLVVADFHTYFVGYRKV
                     LSHDNSVRAPTRAIVPGLTPQ"
     gene            2312..3208
                     /locus_tag="VFV87_09195"
     CDS             2312..3208
                     /locus_tag="VFV87_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5103972.1"
                     /translation="MPELPDISSYLVALRPRIVGQPLEKILVKGPFFLRTVEPDLISL
                     EGRPVLDLSRIGKRIVWHFPGDLFLVFHLMIAGRFHWRKAGTRPIGKNDLAAFSFGSG
                     TVLLTEASKKKRASLHLLRGKAVLRTLNRGGLEPLDCSLAEFRRALMQENHTLKRALA
                     DPRLFAGIGNAYSDEILHAAGLSPVTLTRRLDEPEIARLYDSTQSVLRLWTERLCLSA
                     ADEFPEKVTAFRPGMAVHGRYRQPCPACGTLVQRIVYAENETNYCPRCQTGGKLLADR
                     SLSRLLKDDWPRTIDDWEEGRS"
     gene            complement(3094..3735)
                     /locus_tag="VFV87_09200"
     CDS             complement(3094..3735)
                     /locus_tag="VFV87_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103973.1"
                     /translation="MLRGLLAAALLAAAVNVFFGAVIFCIGLGQLLTGLASLVATAYI
                     LALGLVLVMCIWALAYRISPGPTKYLTVCGVLGGFLLACLPLWLIHAREEARRNNTRN
                     NLRQLGMDGQLVRDSRRDVEPSNVNRPSLPWAELLSTENPWTEVTSTESPWMGLISPA
                     SSWNQEQWNQEQWGSTYDLPSSQSSMVRGQSSFNNLDSERSANSLPPVWQRGQ"
     gene            complement(3750..4154)
                     /locus_tag="VFV87_09205"
     CDS             complement(3750..4154)
                     /locus_tag="VFV87_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5103974.1"
                     /translation="MAKEKDTAVAEKKSQTKRILLVDDDAEIVDAMRYALEKQGYQIF
                     IARDGNQGLAMAEREDPDLVILDMMMPKRSGFLVLEKLRRSREAPVRVIMVTANEGSR
                     HKAYAEMLGVDDYIRKPFAMDKLVDAVQRLLA"
     gene            4341..>4892
                     /locus_tag="VFV87_09210"
     CDS             4341..>4892
                     /locus_tag="VFV87_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5103975.1"
                     /translation="MTTRFSDHELADYQRDGFSIVRRLFDQGEIDKLLQFAKKDPSFV
                     GNVYGRKDAAGHETKLALWNHAGDDLYSMFARSPRIVDRMEQVLEGEVYLYHMKMMLK
                     EPRVGGAWEWHQDYGYWYHNGCLLPLLASCLIAVTRANQANGCLQVIRGSHLMGRIDH
                     GKTGDQTGADMERVHVALERMALL"
BASE COUNT          948 a         1556 c         1493 g          895 t
ORIGIN      
        1 gcacgaggac atgctcgcca ccgcccgcga agtggcgcgg ctgcggatcg acgcggtcaa
       61 gattcataac ttgtacgcgg tgaagaacac gccgctggcc gatcaggtcg agcggggtga
      121 agtgtcgttg atggagcggg acgactacat ccgcaccctc gtcgattttc tggagctctt
      181 gccgccgaag atgatcgtcg agcggatcag cggcgatgct ccgccggact acttcatcgg
      241 cccttcctgg tgccttgaca agcccgccat cctccgcgcc ctgcagcacg aattgcagcg
      301 acgcgatacc tggcagggaa ggcactttcc aatcgaacca gagccatcca cgcacaaact
      361 cattgtgcgg cagccaacgc aggcttagga tagcgtcgat caggggcaag cgacgttcgt
      421 cgcgattcgg atgcgacgtt gcgctaaaca gaggaggtgc tggcatgtcg gtacaatccc
      481 gtttgcgtac tttcttgagt ggttgttccg ggcccctgct gatccttggg atgggactgg
      541 ccctacccgc ggcccttcgg gctgaccctg tcgcacgcct ttccacgcct gctgccgatg
      601 cagtccggga agcgctgcag cgggaaatct acggcttaga gaccgatcgc cgcgagctat
      661 tggccgctgc ggctgagcag gccccgcaat acggaccggc ccgctggcat ctaggttttg
      721 tgcaggacgg taagcatggc tggttgcaac atgaagccgt cctgcaatcg ccgaagcttt
      781 ttcaggagct taccgcatac gaacggcagc gctcgaaggc cttcgacacc gtcgcgggac
      841 aactggcact ggccgagtgg tgccgggagc acgagctggc cgaccaggcg cgggcccacc
      901 tgacgcgcgt gattgatctg aatcccgatc acgctttggc gcgtgctcac ctgggatttg
      961 tccgtcaaaa cggtggctgg gtcagcagcg cggaaatcaa ccgcgacgcc acgcaggaat
     1021 ccgcccgcca ggcagcgctc cgcgtgtggc agccccgaat tgccgcaatc cgcaaagact
     1081 tggagcatcg cagccaggag cgccgtgaat tcgctcagaa taggctggcg gaaattgtcg
     1141 atcccgcagc cgttcctgcg atcgaggcgg tctttgccga cgcttcagag gacgtggccg
     1201 ttcacgccct ggatgcgctg gccaagattt ccgatcccga ggcctcgctg gccctcgccc
     1261 ggttcgctgc ttattcgcca gggcagacgg tgcgcgaggc ggccgccaaa aggctgggca
     1321 agcgcgagct cgaaagctat gtgccgcaaa tcctcgcctc catgtattcg ccgcttgtgt
     1381 cgcgttttat ggccgtgacg ctgcccaccg gtcgcatcgg ctatcgacac gcgttcgtgc
     1441 gagagacgca cgaccgccag gagctgctgc tgctcgatac cgagtatcgc cgccaggcgc
     1501 tgatcggcgg cagccggggc gaaagcactt cgcgcgccct cgatcaggcg gcagacctgg
     1561 cgcgccagcg cgagcaggct gctgcagctc agaacgagtg gatcaaccgg gtcaacgacc
     1621 gattggcgtg ggttctgcag acggcgacgg gccagaatct gccagccgcg cctgaagcct
     1681 ggtggaactg gtggaacgaa cgaaatgagg tgttcctggc gggctccaag ccggtcaaca
     1741 ccattcaaca aacgtcgcaa gtgacaatcg tcgatagcgt gctcattggc acctcagggc
     1801 aacttggcac ttcaggctcc agcacgccga ctccacagac gcacgagtgc ctggccgcgg
     1861 gcacgctggt ttggacggtc aaaggtccct gggaaatcga tcaggttcgc gtcggggatc
     1921 tggttttggc gcaacacccc gagacgggag agctcgcctt caagccggtc ctgcgcacga
     1981 ctgtccggcc caagggtcgg ctgatcaact tgcaatccgg cggcgaatcg tgccagtgca
     2041 gcggcgggca tccgttttgg gttgccggag agggctgggt gaaggcgcgc gagctgcgct
     2101 ccggccaagt cttgcacggc gcgatgggac cggtgcaaat cagcagcgtc gagccttcga
     2161 tcgaggccga aacctacaat ctggtcgtgg ccgatttcca cacgtacttt gtcggctatc
     2221 gcaaggtcct ctcgcacgac aacagcgtgc gggcgccgac gcgggctatc gtcccggggt
     2281 tgacgccgca gtagattccc ctcgcgcggc gatgcccgag ctgcccgaca tttcgagcta
     2341 tctggtcgcc ttgcggccac gaattgtcgg gcagccgctc gagaagatcc tcgtcaaggg
     2401 cccgtttttt ctgcggacgg tcgagccgga cctgatttcg ctcgaaggac ggccggtgct
     2461 ggacctgtcg cggatcggaa agcgaatcgt ctggcatttt ccaggcgatt tgtttctcgt
     2521 ctttcatctg atgatcgccg gccggtttca ttggaggaaa gccggcaccc ggcccatcgg
     2581 caaaaacgat ctggcggcat tctcgtttgg cagcggaacc gtgctgctaa cggaagccag
     2641 caagaaaaag cgagcgagcc tgcatttgct tcgcgggaag gcggtgcttc gaacactcaa
     2701 ccgcggcggc ctcgagccgc tcgattgttc gctggccgaa ttccgtcggg ccctcatgca
     2761 agagaaccac acgctcaagc gggcgctggc cgatccacga ctattcgcgg gtatcggcaa
     2821 cgcctacagc gacgaaatct tgcacgccgc cggtttgtcc cccgtgacgc tgaccagacg
     2881 gctggatgag ccagaaatcg cccggctgta cgactcgacg cagagcgtcc tgcggctctg
     2941 gaccgaacgg ctgtgcctct ccgcagcaga cgaatttccg gagaaggtga ctgcctttcg
     3001 ccccggaatg gccgtccacg gccgctatcg gcagccgtgc ccggcgtgcg gcacgctcgt
     3061 ccagcggatc gtttatgccg agaacgagac gaactactgc ccgcgctgcc agacgggcgg
     3121 caagctgttg gccgaccgct cgctgtcgag attgttaaag gacgactggc cgcggaccat
     3181 cgacgattgg gaggaaggga gatcgtaggt tgaaccccac tgctcttgat tccactgttc
     3241 ctgattccag gacgatgcag gcgaaatcaa ccccatccaa ggactctcgg tggacgtgac
     3301 ctcggtccag ggattctccg tcgagagcaa ctccgcccag ggaagggacg ggcgattgac
     3361 attggacggt tccacgtctc ggcggctgtc gcgaacaagc tggccatcca tcccaagctg
     3421 gcgcagattg ttccgagtat tgttacgccg cgcttcttca cgcgcgtgta tcaaccacag
     3481 cggcaagcag gcgagcaaaa acccgcccaa cacgccgcaa accgtcaggt acttggtcgg
     3541 cccgggcgaa atccgatagg ccagcgccca aatgcacatc accagcacca gacccagcgc
     3601 gaggatatac gccgtcgcga cgagcgatgc caaacccgtc aaaagctgcc ccaacccaat
     3661 gcagaagatc accgcaccga aaaacacgtt caccgccgcc gcgagcaatg ccgccgccag
     3721 cagtccccga agcatggctt gctcctcatc tacgccagca gtcgctgcac ggcatcgacc
     3781 agcttgtcca tcgcgaacgg cttgcggatg taatcgtcga cgccgagcat ctcggcatag
     3841 gccttgtgcc gcgagccctc attggcggta accataatca cccgcaccgg cgcttcgcgc
     3901 gatcggcgga gcttctccag cactaaaaac ccactccgct tgggcatcat catgtcgaga
     3961 attaccaggt ccgggtcttc ccgttcggcc atcgccagtc cctggttgcc gtcgcgggcg
     4021 atgaagatct ggtagccctg cttttccagc gcgtagcgca tcgcatccac gatttccgca
     4081 tcatcgtcca caagcaaaat tcgcttcgtc tgcgacttct tttcggcgac ggctgtgtcc
     4141 ttttccttgg ccatacactt tcctgaaagg tgcatcgcat gaccgcggcg gttaaccccg
     4201 gcgatagcac cgcatgtctc ggttaacccc ggcggccacg ccgggagcga gcaactactg
     4261 aagcttcgtg ttttagctta taacagcgtt ctcgcgcagg caatctggtg aaccacaaac
     4321 cgcgcgggag gaatgcgcgc atgacgactc gtttttccga tcacgaactg gcggactacc
     4381 agcgcgatgg gttttctatc gtccgccgac tgttcgacca gggcgaaatc gacaagctcc
     4441 tgcagtttgc caaaaaagac ccgagctttg tcggcaatgt gtatggccgc aaggacgccg
     4501 ccggccacga gaccaagctc gccctctgga accacgccgg cgacgacctc tacagcatgt
     4561 ttgcccgctc cccgcggatc gtcgatcgca tggaacaggt cctcgaaggc gaagtctatc
     4621 tttatcacat gaaaatgatg ctcaaggaac cgcgcgtcgg cggcgcctgg gaatggcatc
     4681 aggactacgg ctactggtat cacaacggct gcctgctgcc gctcttggcg agctgcctga
     4741 tcgccgtcac gcgggccaac caggccaacg gctgcctgca ggtgatccgc ggctcgcacc
     4801 tgatgggcag aatcgaccat ggcaagacgg gtgaccagac cggggccgac atggagcggg
     4861 tccatgtcgc cttggagcgg atggccctcc tg
//
LOCUS       DASUGB010000478         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1166204, whole genome shotgun sequence.
ACCESSION   DASUGB010000478 DASUGB010000000
VERSION     DASUGB010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166204"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFV87_09215"
     CDS             <1..618
                     /locus_tag="VFV87_09215"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5103976.1"
                     /translation="WHFPRTRLEAELVRDSALHMSGLLSGKLGGPSVFPEQPKSVTAE
                     GAYGALGWNVSPGEDRYRRGLYTFAKRTAPFAMFATFDGPSGEACIARREVTNTPLQA
                     LTMLNEPTLLEAAQALGAKAATSGRGSAETVTDIFRRCLTRPPTEQELSLLVNYYETQ
                     KSRLAAKELDAEKIAGSGDGSAIDRAAWTLTARAILNLDETITKE"
     gene            635..2101
                     /locus_tag="VFV87_09220"
     CDS             635..2101
                     /locus_tag="VFV87_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5103977.1"
                     /translation="MTKQIQNPKSKIQNPLTARRWFLSQCRIGLGAAALGQLLGPNIA
                     SAAETPTRSVSEGVLPTRSPHFPAKAKAVIHLFMAGAPSQLDLFDYKPKLAEYEGKPI
                     PPEVIGGQRYAFIRADAAVLGPRFKFARHGQCGAELSEMLPHLAKVVDEIAIVKSVRT
                     DQFNHAPAQIFLNTGFGQPGRPSLGSWVLYGLGAETQELPAFIVMSTGSGISGGAANW
                     SSGFLPTVYSGVRLRNQGDPILNVSSPEGIDARLQRDTLDLVGQLNRQRLAAIGDPEI
                     ATRIASYEMAYRLQTSAPELMDLKTEDQKTLDLYGCKPAEPSFARACLLARRMVERGV
                     RFINIYHEGWDAHSDVSGNLKNNCGKTDQASAALVQDLKNRGMLDETLVIWGGEFGRT
                     PMVETNAALGRSLGRDHHPQAYTMWLAGGGIQPGLTHGQTDDLGFNVVDGLVHTHDLQ
                     ATILHQLGLDHERLTYRYAGRDFRLTDVHGKVVREILA"
BASE COUNT          464 a          851 c          682 g          382 t
ORIGIN      
        1 tggcatttcc cccgcacgcg gctggaggcc gagttggtcc gcgattcggc cctgcacatg
       61 agcggcctcc tgtccggcaa gctcggtggc cccagcgtct tccccgagca gccgaagtcg
      121 gtcacggcgg aaggggcgta cggcgcgctc ggctggaacg tcagtccggg cgaggaccgc
      181 tatcgccgcg ggctctacac gttcgccaag cgcacggccc ccttcgcgat gttcgccacg
      241 ttcgacggcc ccagcggcga agcctgcatc gcccgccgcg aagtgaccaa cacgccgctt
      301 caggccctga ccatgctcaa cgaacccacc ctcctcgaag ccgcccaagc ccttggcgcc
      361 aaagccgcga ccagtggtcg cggttccgct gaaaccgtga ccgacatctt ccgccgttgc
      421 ctcacccgtc ctccgacgga acaagaactg tcgctgctag tgaactacta cgagacccaa
      481 aagtcccgcc tcgccgccaa ggaacttgac gccgaaaaga tcgcgggctc aggcgacggc
      541 agcgcgatcg accgcgccgc ctggacgctc accgcccgcg cgatcctcaa cctggacgag
      601 acgattacaa aagagtgacc aagcaccaac tccaatgacc aaacaaatcc aaaatccaaa
      661 atccaaaatc caaaatcccc tcaccgctcg ccgctggttc ctctcccagt gccgcatcgg
      721 cctcggcgca gccgcccttg gccaactcct cgggcccaac atcgcgtccg ccgccgaaac
      781 accaacccga agcgtcagcg agggagtgtt gccgacccgc tcgccccact ttcccgccaa
      841 ggccaaagcg gtcatccacc tcttcatggc cggcgcgccg agccagctcg acctgttcga
      901 ctacaagccg aagctcgccg aatacgaggg caagccgatc ccgcccgaag tgatcggcgg
      961 ccagcgttac gccttcattc gcgccgatgc ggcggtactc ggcccgcggt tcaaattcgc
     1021 caggcacggc cagtgcggcg cggagctttc cgagatgctg ccgcacctgg caaaggtcgt
     1081 cgatgagatc gcgatcgtca aaagtgtccg caccgaccag ttcaaccacg ccccggcgca
     1141 gatcttcctc aacacgggct tcggccaacc gggccggccg agcctcggct cgtgggtgct
     1201 gtatggcctg ggggcggaaa cgcaggaatt gcccgcgttt atcgtcatgt ccaccggcag
     1261 cggcatcagc ggcggggccg ccaactggag cagcggcttt ctgccgacgg tctattccgg
     1321 cgtgcggctg cggaaccagg gggatccaat tctgaatgtc agcagccccg agggaatcga
     1381 cgcgcgtctg cagcgcgaca cgctcgacct cgtcggccag ctcaatcgcc agcggctcgc
     1441 ggcgatcggc gaccccgaaa tcgccacccg catcgcctcg tacgaaatgg cctaccgcct
     1501 gcaaaccagc gcccccgaac tgatggacct caagaccgaa gaccagaaaa cgctcgatct
     1561 ctatggctgc aaacccgcag agccgtcgtt cgcccgggcg tgcctgctcg cgcggcgaat
     1621 ggtcgagcgg ggggtgcgat tcatcaacat ctaccacgaa ggctgggacg cccacagcga
     1681 cgtctccggc aacctgaaga acaactgcgg caaaaccgat caggcgagcg cagccctcgt
     1741 gcaggatctg aagaaccgcg gcatgctgga cgagacgctg gtcatctggg gaggcgaatt
     1801 cggccgcacg ccgatggtcg aaaccaacgc cgccctcggc cgcagcctcg gccgcgacca
     1861 ccacccgcag gcctacacga tgtggctcgc cggcggcggc atccagcccg gcctcaccca
     1921 cggccagacc gacgacctcg gcttcaacgt cgtcgatggc ctcgtccaca cccacgacct
     1981 ccaggccacc atcctccacc agctcggcct cgaccacgaa cgcctcacct accgctacgc
     2041 cggccgcgac ttccggctaa ccgacgtgca tgggaaggtg gtgagagaga ttcttgctta
     2101 acaacgcagt tgaggcgcat catgaatcgt ccattgtggg ttgaagtgct cgagctatcg
     2161 gctttcattg gtggttgcac gtttgtggcg acggtcgcca cgctactcat ttgcaattcg
     2221 tttgatcttg gttcggtcgc cgcgatgtta ctgttcgtac cgttgctcgc attgtcgtgc
     2281 gcattgccct acggattctc ggtcgttatt gaacgattcc gaacaagata aggtcggatt
     2341 cgaggacacc tgagttgccc caacggggca acctgcgcc
//
LOCUS       DASUGB010000479         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1583919, whole genome shotgun sequence.
ACCESSION   DASUGB010000479 DASUGB010000000
VERSION     DASUGB010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583919"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2850
                     /locus_tag="VFV87_09225"
     CDS             <1..2850
                     /locus_tag="VFV87_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5103978.1"
                     /translation="PQSIRWKFERRFPSPSGIRSAIVRVAADGCNLIVSVNGQKTLQL
                     QPFSPTVELQVAQHLRAGENRIEVLADSTASPAAFAASLLLRTGSGEPLEIVTDEDWR
                     VTPAAAERATGDTPATSLGLVAPELWGLNLRPATIDPFDNYEQWRQALGDAPGGKSPS
                     FWTAPEFEITLLRSAQPEEGSWVSLAFDPRGRLTIAREDKGLLRMTLDADSRAVAKVE
                     AINDELLECRGLLYAYDALYANANNSKGLYRLRDADGNDQFDDVQLLREFPGGVGHGR
                     NDLALGLDGLIYSIHGDAVDVPTADIADRTTPFRNARRGQKTSEGCLLRTDQGGKEWE
                     LVCGGMRNPFGIAFNAAGDLFTYDADAEFDMGTPWYRPTRVLQLRSGADFGWRGVTGN
                     WPPYFPDQPEHALPALDIGKGSPTAVAFGSGSKFPPPYQGALFILDWAYGRVLAVHLA
                     PRGAGYRSQAETFLKGRPLNVTDLAFGPDGAMYLVTGGRKTQSALYRVAYVGQPMQPP
                     ELSRHEQLCQSQAIAARKLLRELEALHRPSEMAVEKAWPHLDAADPVIRHAARIAIEH
                     QPVERWRDRAWAEKRTNASLEALLALAGSGETEPYPQIIERLLAYPFAELNVSQSLAL
                     LRIYQLCWEANPESLAPQRTQIVAQLDAAFAPAERPPLRVAPGGTSNHVQRDLARLLV
                     QLGSPTAVEKTVRTFLRSDMQEDRLYALFVLRHEREGWTLDMRREYFAALNEGENFLR
                     GEGMPRFLTQLREEATQSLAPEERKALGEIVSASVEAAEADLLLPSRAVVKAWTIDDF
                     KALLSVSPPRGDATRGEAVFREALCARCHRVGARGPAVGPDLTHVAGRFSRQDILESI
                     LTPSKVVAENYRNVQVVLMDGQSLVGRVLNEGDYRSETLRLAPDPLRPSHVVEINKRD
                     IERYQLSETSPMPKGLLDTFTAEEVLDLLAHLRGP"
     gene            complement(2927..>3160)
                     /locus_tag="VFV87_09230"
     CDS             complement(2927..>3160)
                     /locus_tag="VFV87_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5103979.1"
                     /translation="DVRLKAVEVLARMGSDANPAVPDLVALLDDPDERIRKAATRTLG
                     RIGPEAAPAVPALMRSLLQPEPSLPALQPVPVE"
BASE COUNT          541 a         1048 c         1029 g          542 t
ORIGIN      
        1 ccgcagtcga tccggtggaa atttgagcga cggttcccat cgccaagcgg cattcgcagc
       61 gctattgtgc gggttgccgc agacggatgc aacctgattg taagcgtcaa tggccaaaag
      121 actttgcaac tgcagccctt cagccccacg gtcgaacttc aggtcgccca gcatctgcgg
      181 gccggcgaaa accgcatcga ggtcctggct gattccactg cctccccagc cgcctttgcc
      241 gccagcctgc ttctgcgcac tggctccgga gagcctctgg agatcgtgac tgacgaggat
      301 tggcgcgtca cgccggcggc ggccgagcgc gcgacaggcg acacgccggc aacttcgctc
      361 ggactggttg cgccggaatt gtggggtctg aatctccggc cggcaacgat tgaccccttc
      421 gacaactacg agcagtggcg gcaggccctg ggcgatgccc ccggcggaaa gtcgccttcg
      481 ttctggaccg ctcccgagtt cgaaatcacg ctgctgcgct ccgcgcagcc cgaggaaggc
      541 tcgtgggtca gcctggcgtt cgatccgcgg ggccggctga cgatcgcccg ggaggacaag
      601 ggccttttac ggatgacctt ggatgcggat tcgcgggcgg ttgccaaggt ggaggcgatc
      661 aacgacgaac tactcgagtg tcgcggcttg ctctatgcct atgacgctct ttacgccaat
      721 gcgaacaatt ccaaggggct ttatcgactg cgcgacgccg acggcaacga ccagttcgac
      781 gacgtgcaac tgctgcgcga gtttcccggc ggcgtgggac acggacggaa cgatctggcg
      841 ctcggtcttg acggcctcat ctattccatc cacggcgacg ccgtcgatgt tccgacggcg
      901 gacatcgccg accggacgac gcccttccgc aacgcccgcc gcggccagaa aaccagcgaa
      961 ggctgcctgc tccgcacgga tcagggcggc aaagagtggg aactcgtctg cggcggaatg
     1021 cgaaatccgt tcggcatcgc cttcaacgcg gctggagatc tcttcactta cgacgcggat
     1081 gccgaattcg acatgggcac accctggtat cgcccgacgc gcgtgctgca gcttcgctcg
     1141 ggggccgatt tcggctggcg cggcgtgacc ggcaattggc ccccttattt ccccgatcag
     1201 ccggagcacg ccctgccggc gctcgatatc ggcaagggct ctcccaccgc ggtcgccttc
     1261 ggcagcgggt cgaaatttcc gccgccgtat cagggcgcct tgttcattct cgactgggcc
     1321 tatggccgcg tgctggccgt tcatctggcc ccgcgcggcg cgggctaccg ctcccaagcc
     1381 gagacgttcc tgaaaggccg cccgctcaac gtgaccgatc tggctttcgg cccggatggc
     1441 gcgatgtatc tggtgactgg cgggcggaag acgcagtcgg cgctctacag ggtcgcgtat
     1501 gtcggccagc caatgcagcc acccgagcta agccggcatg aacagctttg ccagtcgcag
     1561 gcgatcgccg ctcgcaagct gctgcgtgag ttggaagcgt tgcaccggcc ctccgaaatg
     1621 gctgtcgaga aagcctggcc gcatctggat gccgcggatc ctgtcattcg ccacgccgcc
     1681 cgcatcgcga tcgagcacca gccggtcgag cgctggcgcg atcgcgcctg ggccgagaag
     1741 cgaaccaacg cgagcctgga agccctgctc gctttagccg gcagcggaga aaccgagccg
     1801 tacccacaga tcatcgagcg cttgctcgcg tatccgtttg ccgaactgaa cgtgagccaa
     1861 tcgctcgccc tcctccgcat ctaccagctt tgctgggagg caaacccgga atctctcgcg
     1921 ccgcagcgga cgcaaatcgt tgcccagctc gacgcagcct tcgcaccagc ggagcggccg
     1981 ccacttcggg ttgctccagg cggaactagc aatcacgtgc agcgtgatct ggcccggctg
     2041 ctcgtgcaac tcggctcgcc cactgcagtc gagaagacgg tccgcacgtt cttgcggagc
     2101 gacatgcaag aggatcggct gtatgccctg tttgtgctgc gccacgagcg cgaaggctgg
     2161 acgctcgaca tgcgccggga gtatttcgcg gcgctgaatg aaggcgagaa ttttctgcgc
     2221 ggcgaaggga tgccccggtt tctgacacaa ttgcgcgagg aggccaccca atcgctcgcg
     2281 cctgaagagc gcaaagctct gggagagatt gtctcagcct ccgtcgaagc agccgaagcc
     2341 gatctgctgc tgcccagccg agctgtggtg aaagcctgga cgatcgacga tttcaaagcg
     2401 ctgctgtcgg tcagcccgcc gcgcggcgat gcgactcgcg gcgaggcagt ctttcgcgag
     2461 gcgctgtgcg cccgctgcca ccgcgttggg gcgcgcggac cggcggtcgg gcccgatctg
     2521 acgcacgtcg ccggccgctt cagccggcag gacatcctgg aatcgatcct gactccgtcg
     2581 aaggtcgtgg ccgagaacta tcgcaacgtg caggtcgtgc tcatggacgg ccaatcgctc
     2641 gtcggccgcg tgctgaacga gggggactat cgctccgaga cattgcggct ggcgcccgac
     2701 ccgctgcggc catcgcatgt ggtcgaaatc aacaaacgcg acatcgagcg gtatcagctt
     2761 tcggaaacgt cgccgatgcc caaggggctg ctcgacacct tcaccgcgga agaagtgctc
     2821 gatctgttgg cgcacctgcg cgggccgtag ccgcgttcgc cagaacgcgg gcgccggggg
     2881 atttcccgca ctctggcgag tgcggctacg gccgaggatt cgttgctcac tccaccggca
     2941 ccggctgcag ggccggcagc gatggctccg gttggaggag cgagcgcatc agcgccggca
     3001 cggcgggggc tgcctcggga ccgatccgcc ccagcgtccg cgtagccgcc ttgcggatgc
     3061 gctcgtcggg atcgtcgagc aaggcgacca gatcgggcac agcggggttg gcatccgagc
     3121 ccattcgggc cagcacttcc accgccttga ggcgcacgtc
//
LOCUS       DASUGB010000480         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_749166, whole genome shotgun sequence.
ACCESSION   DASUGB010000480 DASUGB010000000
VERSION     DASUGB010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749166"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFV87_09235"
     CDS             complement(<1..521)
                     /locus_tag="VFV87_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103980.1"
                     /translation="MTPADRIRNCEPSVVPPSQLPLPGAPPSGPSVWPEDLREDSWWP
                     VSDQGRTGACVGFGVGDGLIRWHLVKKGRLAPEEALSVRYFWMAAKEMDEYGDWPTTF
                     LEEEGTSIWAALRMAMLYGCLLESELSLDGKLFQGSKDQFLNVASRRKISKVHPLELD
                     AKSWANWLRIHGPL"
     gene            complement(695..1942)
                     /locus_tag="VFV87_09240"
     CDS             complement(695..1942)
                     /locus_tag="VFV87_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HEX5103981.1"
                     /translation="MSTTELTGDQHACLELPVFNPDDSPETKSRSPTELAYMAQQGTL
                     ASLPARAAQLAAENYPAGIQSAPARAAFANHLELADKILRFYFQPGTPANLADRIIEH
                     ARTWTKHIDLKFDVVHDPARAVVRVRCSRLVGHASHIGSDGLRVPVNQETMTLGFSDL
                     IDFEKQPKYNEFVVLHEVGHALGLIHEHNRGDAALDFDRGRTIAYFATRGLNAAQVQY
                     NIFDRWTKPLVKFSEYDPKSIMHYMFPKEIMRSGQGTAQNFELSPNDIRMAQEMYGAA
                     DGSPPPPAPPRPTSGETQMAIDGAPAAVNLRPGQAVTLKFQVAAGQGGKPYTIYSEGG
                     TQVVLQLLGPNDATKDATPASAPGHGTYDLTNDAIQATLAEGAYVVKVQHVSSHGGGN
                     TAVAVTSGEKFGSRVLNANRPGT"
BASE COUNT          342 a          647 c          651 g          426 t
ORIGIN      
        1 agcggcccat gaatgcgcag ccagttcgcc cacgatttcg catccagctc cagcgggtgg
       61 accttgctga tcttccgccg actggccacg ttcaggaact gatccttgga gccttgaaac
      121 aatttgccgt cgagtgacag ctcgctctcc aacaggcagc catagagcat ggccatgcgc
      181 agcgccgccc agatgctggt gccttcttcc tccaggaagg tcgtcggcca atcgccgtac
      241 tcgtccattt ccttcgccgc catccagaag tagcgcacgc tgagagcttc ctccggcgcg
      301 aggcgcccct ttttcacgag atgccagcga atcagtccgt cccccacgcc gaaccccacg
      361 caggcgccgg tgcggccctg atcgctcacc ggccaccagg aatcttcccg caggtcctcc
      421 ggccagacgg aaggcccgct aggcggagcg ccgggcagcg gaagttgact cggaggaacg
      481 acagacggct cgcaattccg gattcgatcg gctggggtca tggagaattc cacctcggcc
      541 gattgggccc caggcagagt tgcagtggat ttcgagctgg cgcgcttcgc tctggacgtg
      601 cgcttctgtt gctttccctg ggcgctattc gatttggccc tttttttcgt cttcttcgcc
      661 acggagtgtc tcccgccggc atcgcgcgcc gcgatcacgt gccggggcga ttggcgttca
      721 gcactcggct gccgaatttc tcgcccgatg tgaccgcgac ggccgtgttc ccgccgccgt
      781 ggctggagac gtgctgaacc ttgacgacat acgcgccctc ggcgagcgtg gcttgaatgg
      841 catcgttggt caggtcgtac gtgccatggc ctggtgcgct ggcgggtgtc gcatccttgg
      901 tcgcgtcgtt ggggcccagc aactgcagca cgacttgcgt cccgccttcc gagtagatcg
      961 tgtagggctt acccccctgg ccggcggcga cctggaactt caacgtcaca gcctggcccg
     1021 ggcgcaaatt cacggctgcc ggagcgccgt caatcgccat ctgggtttcg cccgaagttg
     1081 gccggggcgg agcaggcggc gggggcgaac catcggcggc gccgtacatc tcctgggcca
     1141 tgcgtatgtc gtttggcgag agctcgaaat tctgtgccgt cccctggccg ctgcgcatga
     1201 tctccttcgg aaacatgtag tgcatgatcg acttggggtc gtactcgctg aacttgacca
     1261 gcggcttcgt ccagcggtcg aagatgttgt attggacctg ggccgcgttc aggccgcggg
     1321 tggcgaagta ggcgatcgtc ctgccccggt cgaaatccaa cgccgcatcg ccccgattgt
     1381 gctcgtggat gagtcccagc gcatgtccca cttcgtgcag caccacgaac tcgttgtatt
     1441 tgggctgctt ctcaaaatcg atcaaatcgc tgaagccgag cgtcatcgtc tcctggttca
     1501 ccggcacgcg cagcccatcg gaaccgatgt gcgaagcgtg tcccaccagc cggctgcagc
     1561 ggacgcgaac gacggcccgc gccggatcgt ggacgacgtc gaacttcaga tcgatatgct
     1621 tcgtccaggt ccgtgcatgt tcgataattc gatcggccag gttcgcgggc gtccccggct
     1681 ggaagtaaaa ccgcaggatc ttgtcggcca actccagatg attggcgaac gctgctcggg
     1741 cgggagccga ttggatgccc gcggggtagt tctctgcagc gagttgcgcc gctctggccg
     1801 gaaggctggc cagcgtcccc tgctgcgcca tgtaggccag ttctgtggga gaacgggact
     1861 ttgtttccgg ggaatcatcg ggattgaaaa ctggaagttc cagacaggcg tgctggtcgc
     1921 cagtcagttc ggtggtgctc ataatgggcg cctcgtgagt cgggaggaga ccacccctgc
     1981 gaatgcggga tagggctggt tcgcccagat tatcccgagt cgcggaccgg ttttcaacat
     2041 ttgcgagcgg cattggtaat ttataa
//
LOCUS       DASUGB010000481         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_194196, whole genome shotgun sequence.
ACCESSION   DASUGB010000481 DASUGB010000000
VERSION     DASUGB010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194196"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..588
                     /locus_tag="VFV87_09245"
     CDS             313..588
                     /locus_tag="VFV87_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103982.1"
                     /translation="MDRPEEIIIPVAVNAMIFGTLMLWIIAHYGLSAWKHWRTTSLIR
                     DMVARGYTAAEIIQICQALGHKKPCLDPKKVMDVPPAKPIQQPAFSP"
     gene            692..2077
                     /locus_tag="VFV87_09250"
     CDS             692..2077
                     /locus_tag="VFV87_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5103983.1"
                     /translation="MFPPWRFKLKEAQVALEQGRLEEAARAVEEPDLRQFLPVQQTLA
                     EVADQMARRALERAAAGDIAAGWRDLEEAKKLGGESADWLRHRQAVAEVSVGEIVNHL
                     KAGDFTGAVARIDSLDKRKIPGLPLQALLEVTRRLDSARKLAARSKFAEADEQLAAAS
                     ALQPDLPLIDEQRQLYRERADRSRSLTEQLHAALAANDWTKVLAIATELLEIAPEHRV
                     ARDARKRAWAAVGERIGDSRRMGETHHWTGTPNSNAMASVASPESPEKRAPRFMLWID
                     AVGGYLVCLGDEVTIGQAVPNNRVDVPILADLSRKHAKICRRGEGYVLEPLGHKLVLD
                     GKPVTGPTLLSDGDEFVLSEAVKLRFRKPHALSASARLEMLSPHRTQPGADAVILMAE
                     SCVLGPKWQNHVVCRDWNGDVVLYRSDHNIMCRAMESIEIDGRLHDGRGPVRPGSHVL
                     GTDFSLSLEAV"
     gene            2077..2307
                     /locus_tag="VFV87_09255"
     CDS             2077..2307
                     /locus_tag="VFV87_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103984.1"
                     /translation="MQKLITIYVDNSAYGQGKMIVTSYADRHGFVEEHLKAELAHGWN
                     VKSLHGFGGNSDGLGVRGWIVALLENPSDAAV"
     gene            2688..>3780
                     /locus_tag="VFV87_09260"
     CDS             2688..>3780
                     /locus_tag="VFV87_09260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5103985.1"
                     /translation="MITRAATIDPAGPENGREAAAQKGNAMKFLYASGSRPLEGYTIK
                     RGIGRGGFGEVYFATSEAGKELALKHVQRNLEVELRGVGQCLNLKHQNLINLYDIRYD
                     EQGDAWVVMEYVAGDSLKDVIDRNPNGLPLDQVEFWFRGIAAGTAYLHDHGIVHRDLK
                     PGNIFEDSGCVKIGDYGLSKFISTSRKSAQTESVGTFHYMAPEIGKGVYGKEIDIYAL
                     GVVLYEMISGAVPFDGESSQEIIMKHLTADPDLSGIPQPYRSIIERALQKDPQKRFTS
                     VKEMISALDNVPLAGTKPGPGVSPPASPGAADLLYIGAEDEGIELGPLELHPENNPVT
                     AELVKANGRHASRPASPAGGEEPIAKAVKD"
BASE COUNT          738 a         1187 c         1212 g          643 t
ORIGIN      
        1 gtcgcggtga agctggacta agcgtggggc ggacgtctcg cctgccctgg aaatcctcgg
       61 tcccaagctc ctgcttggga ccgcccgtcc ctcgttccca cgctctgcgt gggaacgcgg
      121 tttcccgccg ctctgcggcc cgcgtgacgc ggagcgtcga agacttgcgt tcccacgcgg
      181 agcgtgggaa cgagcgaaca ccacgattcc agcaacccgg aatcgctgca gtcctcgcca
      241 ccgcaccggg cgggcgcgag gatttgcctt taggatcggc ctcggcgaaa atcgcagtcg
      301 gagggcttgg ccatggatcg ccccgaagag atcatcattc ccgtcgctgt gaacgccatg
      361 atcttcggca cgctgatgct gtggatcatc gcgcactacg gcttgagcgc ctggaagcac
      421 tggcggacga cttccttgat ccgcgacatg gtcgctcgcg gttacactgc cgcggagatt
      481 attcagatct gccaggctct cgggcacaag aaaccgtgcc tcgatccgaa gaaggtgatg
      541 gacgttccgc ccgccaagcc catccagcag ccggcgttca gtccctaaga caacaacccg
      601 aagcgtgagc gagggagtgt tgcgacgaca cttcccgcga ctccctcgct gacgcgtcgg
      661 gttggtatga tgcggagcag cgcaggtgag catgttcccc ccctggcgat tcaaactgaa
      721 ggaagctcag gtcgccttgg agcaaggccg gctggaggaa gcggcgcggg cggtggagga
      781 gccggacctg cggcagttcc tgcccgtgca acagacgctg gcggaggtgg ccgatcagat
      841 ggcccgccgg gccttggagc gggcagccgc gggagatatc gccgccggct ggcgcgatct
      901 ggaggaggcg aagaagctcg gcggcgaatc ggccgactgg ctgcgacatc gccaggcggt
      961 cgcggaagtc tcggtcggcg agatcgtgaa tcatctcaag gccggcgact tcaccggcgc
     1021 cgtcgcccgt attgactcgc tggataaacg caaaatcccc ggcctgccgc tgcaagcctt
     1081 gctggaggtc acccgccggc tcgattcggc ccgcaagctg gccgcccgca gcaaattcgc
     1141 cgaagcggat gagcaactcg ccgcggcctc cgccttacag ccggatttgc cactgatcga
     1201 cgagcaacgg caactatatc gcgagcgggc cgatcgctcg cgctcgctga ccgagcagct
     1261 tcacgccgcc ctggccgcca acgattggac gaaagtcctg gcgatcgcga cggaactgct
     1321 cgaaatcgct cccgagcacc gcgtggcccg agatgcccgc aagcgggcct gggccgcggt
     1381 cggcgagcgg attggcgatt cccggcggat gggtgagacg caccactgga ccggaacgcc
     1441 gaactccaat gcgatggcga gcgtcgccag tccagaatcg cccgaaaagc gcgccccgcg
     1501 tttcatgttg tggatcgacg ccgtcggcgg ttatctcgtc tgcctgggcg atgaagtcac
     1561 gatcggccag gccgtgccga acaaccgcgt cgatgtgccg attctggccg acctgtcgcg
     1621 caagcacgcc aaaatttgcc gacggggaga aggctatgtc ctcgaacctc tcggccacaa
     1681 gcttgtcctc gatggaaagc cggtcaccgg accgacgctg ctgtccgacg gcgacgagtt
     1741 cgtcctcagc gaggccgtga agctccgctt ccgcaagccg catgcgctca gtgcgagtgc
     1801 ccggctggag atgctgagcc cgcaccgcac gcagccgggg gccgacgcgg tgattttgat
     1861 ggcggaatcg tgtgtccttg ggccaaaatg gcagaaccac gtcgtctgcc gcgattggaa
     1921 cggtgacgtg gtgctgtacc gcagcgatca caacatcatg tgccgggcga tggagtcgat
     1981 cgagatcgac ggccgcctgc acgacggccg cggccccgtc cggcccggct cgcacgtgct
     2041 ggggaccgat ttttctctgt cgctggaggc ggtgtagtgc aaaagctgat cacgatttac
     2101 gtggacaact cggcctatgg acaaggcaag atgatcgtca cgtcctatgc cgacaggcat
     2161 ggcttcgtgg aagagcacct gaaagcagaa ctcgctcacg gttggaatgt gaagtcgctg
     2221 catggattcg gcggcaacag tgacgggctc ggagtccgcg ggtggatcgt cgccctgctg
     2281 gaaaatccgt cggatgcggc ggtttgagga ataggggcca ggagataggg gtcaccagtt
     2341 cgtaggacgg gtttgtaacc cgtccgcgaa aacgcgtaag tatggagtga attcggaatc
     2401 tggcctcgcg agctgttgag tacaaccttg aaggatttgt tcggacatat cttcgagcat
     2461 gtggtcgttg aactgattgg tcgagtcctc gaatccgctg ctcggtggtg gagttcggag
     2521 gaacgctagg cggatcgcaa cccattattt gacaggaggt tacaacccaa accgtccgtc
     2581 cgcgtccgca tcggcaagat ttccccattc ccagcgaaaa cccactaggt tgcaggctct
     2641 ctgtctctac cggacaggcg cttgtgccag ggggtaaact agaagcaatg atcacccgtg
     2701 ccgcgacgat cgatcctgcc ggcccggaaa acggccggga ggcagccgcc cagaaaggga
     2761 acgcgatgaa gttcctttat gccagcggtt cgcgtccgct cgagggctac accatcaagc
     2821 gcggcatcgg acgcggcggc tttggcgagg tctatttcgc caccagcgag gcgggcaagg
     2881 agctggccct caagcatgtg cagcggaacc tggaggtcga gctccgcggc gtcggccagt
     2941 gcctcaacct gaagcaccag aacctcatca acctctacga catccgctac gacgagcagg
     3001 gcgatgcctg ggtcgtcatg gagtacgtcg ccggcgacag cctcaaggac gtgatcgacc
     3061 gcaatcccaa cggcttgccg ctcgaccagg tcgagttctg gtttcgcggc atcgcggcgg
     3121 gcaccgcgta tctgcacgac cacggcatcg tccatcgcga tctcaagccg gggaacatct
     3181 tcgaagactc cggctgcgtg aagatcggcg actacggcct cagcaagttt atttccacca
     3241 gccgcaaaag cgcccagacc gagagcgtcg gcactttcca ctacatggcc ccggagatcg
     3301 gcaagggggt gtacggcaag gaaatcgaca tttatgccct cggcgtcgtc ctgtacgaga
     3361 tgatttccgg cgccgtgccg tttgacggcg aaagcagcca ggaaatcatc atgaagcacc
     3421 tgacggccga tcccgacctg tcaggcatcc cacagccgta ccgctcgatc atcgagcggg
     3481 cgctgcagaa ggacccacag aagcgattca cgtcggtcaa ggaaatgatt tcggcgcttg
     3541 acaacgtgcc cttagccggg accaaacctg gtcccggcgt atcgccgccc gcttcgcccg
     3601 gtgctgccga cctgctctac atcggcgccg aagacgaagg catcgagctc ggcccgctgg
     3661 aactgcatcc cgagaacaac ccggtcaccg ccgagctggt caaggccaac ggccggcatg
     3721 cctcgcggcc tgcgtcgccc gccggcggcg aagaaccgat cgccaaagcg gtcaaagacg
//
LOCUS       DASUGB010000482         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1442938, whole genome shotgun sequence.
ACCESSION   DASUGB010000482 DASUGB010000000
VERSION     DASUGB010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442938"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFV87_09265"
     CDS             <1..747
                     /locus_tag="VFV87_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX5103986.1"
                     /translation="KTGEYSVLTVTAEGRRLLKGEITPRLLKAPEPKKKEAKVAVASW
                     EGVDRGLFERLRALRRQKAEQRGISPLVVFDEVTLRGLARVRPSNAANLVKVQGIGEK
                     KAAEYGSEFLAAIADYCHEHSLGQDVFDSPAAEQDAKPAKLSPTTSSSKRKAIELFLQ
                     GKTIDEVCQAVNRARSTVSEYLGDAIAMHGISDYSPWLDDALYNRIRDAAQQHNDGRL
                     KPIKETLGDDVSYEEIRIALACLRNAAPEN"
     gene            970..>2386
                     /locus_tag="VFV87_09270"
     CDS             970..>2386
                     /locus_tag="VFV87_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5103987.1"
                     /translation="MLRPLLGLAILISCFELVARAGDADKPVRKPFGLEKRVPWTTSN
                     VVGYPEPPPPYRTERVFPKLSFAEPLDLAAQPGGKRLWVAERRGKLFSFVQDPAVSKA
                     DIALELSTKNDKGEAVPQTIYAFTFHPKFAENGYVYVTWIPDGSKEGIPNGTRVSRFT
                     AKGEPPVIDRASEKVIIEWPNGGHNGGCLKFGPDGMLYIVTGDGSGIADELQIGQDLS
                     SILGKILRIDVDHPAEDKGYSVPKDNPFVAMKDARPETWAYGLRQFWRFSFDRQTGQL
                     WGGEIGQDLWESVHRIQKGGNYGWSVMEATHPFRPERKKGPTPILQPVVEHSHSDFRS
                     ITGGTVYRGKRLPELAGHFIYGDFDTGRIWAFRLGLNLREDDMTVSNAGIPLISDHRE
                     LARTNYRVITFAEDAAGELLFLDFTGGGLHQLVKAPPVEQAAKPFPTKLSQTGLFAST
                     KDHTPTAGLIPYSVNSQLWSDH"
BASE COUNT          477 a          757 c          734 g          418 t
ORIGIN      
        1 aaaacgggtg aatacagcgt gctgaccgtc accgccgaag gccgccgcct gctcaagggc
       61 gaaatcacgc cccgcctgct caaagcgccg gagccgaaga agaaggaggc caaggtcgcg
      121 gtggcttcct gggaaggggt cgatcggggc ctcttcgagc ggctgcgggc gctgcggcga
      181 caaaaagcgg aacaacgagg catttcgccg ctcgtggtct tcgatgaagt cacgctccgc
      241 ggtttggccc gcgtccggcc gtcgaatgcc gcgaacctgg tcaaggtgca gggcatcggc
      301 gagaagaagg ccgccgaata cggtagtgag tttctcgcgg cgatcgccga ctattgccac
      361 gagcactcgc tcggccagga tgtcttcgat tcgcccgctg ccgagcaaga tgcaaagccg
      421 gcgaagctct cacccacgac cagcagctcg aagcggaagg cgattgagct gttcctgcag
      481 ggaaagacga tcgacgaagt ctgccaggcg gtcaatcggg cccgttcgac ggtcagcgaa
      541 taccttggag acgccatcgc catgcacggc atctccgact actcaccctg gttggacgac
      601 gccctttaca atcgcatccg cgacgccgcc cagcaacaca atgacggccg tctcaagccg
      661 atcaaagaga ccctcggcga tgacgtcagc tacgaagaaa tccgcatcgc cctcgcctgc
      721 ctccgcaacg ccgccccgga aaactaaccc gaagcgtaag cgagggcagg aggattgcaa
      781 tgtcgcaaat tcgcgtcgct ggcgggacca taagtccttt agtgtcgtgg tcaccgaccc
      841 ttggctagct tggcttttcg agacttcgca atcctcctgt aagcgagggc gtgcgattgg
      901 cgttcgcccg ctgcggttgc ttacaatacg gccacgactt tccctgcgtc ctcattgtgg
      961 gttcctccaa tgctgcgccc tctccttggc cttgcaattc tcatctcgtg ctttgagtta
     1021 gtcgctcgcg ctggcgatgc cgacaaacct gtccgcaagc cattcggcct ggaaaaacgc
     1081 gtgccctgga cgacctcgaa tgtggtcggc tatccggagc cgccgccgcc gtaccgtacg
     1141 gagcgggtgt ttccgaagct gagctttgcg gagccgctcg acctggccgc gcagccgggc
     1201 ggcaagcgcc tctgggtcgc cgagcgccgc ggcaagctgt tctccttcgt ccaagacccc
     1261 gctgtatcga aagccgatat tgccctggag ctgtccacca agaacgacaa gggcgaagcg
     1321 gtcccgcaga cgatctacgc cttcaccttt cacccgaaat tcgccgaaaa cggctacgtc
     1381 tatgtgacgt ggatcccgga cggctcgaag gaagggattc cgaacggcac gcgcgtgtca
     1441 cggttcaccg cgaaggggga gccgccggtg atcgaccgcg ccagcgagaa agtgatcatc
     1501 gagtggccga acggcggcca caacggcggc tgcctgaagt tcggccccga cgggatgctc
     1561 tacatcgtca cgggcgacgg ctcggggatc gctgacgaat tgcaaatcgg tcaggacttg
     1621 tcgagcattc tcggcaagat cctgcggatc gacgtcgatc atcccgccga ggataaaggc
     1681 tacagcgtgc cgaaggacaa cccgttcgtg gcgatgaagg acgcccggcc ggaaacgtgg
     1741 gcttacggcc tgcggcagtt ctggcgcttc agttttgacc gtcagacggg ccaactgtgg
     1801 ggcggcgaaa tcggccagga cctgtgggag tcggtccacc gcatccagaa agggggcaac
     1861 tacggctgga gcgtgatgga agcgacgcat cccttccgcc ccgaacgcaa gaagggaccc
     1921 acgccgattc tgcagccggt cgtcgagcac tcgcacagcg attttcgctc gatcaccggc
     1981 ggaaccgtct atcgcggcaa gcggctgccg gagttggcgg ggcactttat ctacggcgac
     2041 ttcgacacgg ggcggatttg ggcatttcgc ctgggtctca atcttcgtga agatgatatg
     2101 accgtctcga atgccggaat tccgctcatc agcgaccacc gcgagctcgc ccgcaccaat
     2161 taccgcgtca tcacgttcgc cgaagacgcc gcgggcgaat tgctgttcct tgatttcacc
     2221 ggcggcgggc tgcaccagct cgtgaaagca ccgccagtcg aacaggcggc gaagcccttt
     2281 ccgacgaagc tgagccaaac gggcctgttt gcttcgacga aggaccacac gccgacagcc
     2341 gggctcattc cgtacagcgt caactcccag ctttggagcg accatg
//
LOCUS       DASUGB010000483         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_472517, whole genome shotgun sequence.
ACCESSION   DASUGB010000483 DASUGB010000000
VERSION     DASUGB010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472517"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFV87_09275"
     CDS             complement(<1..561)
                     /locus_tag="VFV87_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103988.1"
                     /translation="MVRCFVSLLISSGVFVTAGASLADDNPFWNLVHDRAVVADLKLT
                     DAQRGEWHAVLDPLDLQVFPLRNQRADAAQPALAKIVDQARAGLSKVLTPKQNARLEA
                     IRVRVLGVRALRNDKVAQQLKLSEMQREKLAAIIDEHDSARAEVQKTADEKPADDVEK
                     ERSRISTEERAAVQKLLTADQLTRLRS"
     gene            complement(712..1605)
                     /locus_tag="VFV87_09280"
     CDS             complement(712..1605)
                     /locus_tag="VFV87_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103989.1"
                     /translation="MLTNWLSSGIFLLFGLRIVAAVDVPPTKPAPAPISWLSDYRDAL
                     DRGESSSKMVLIWFVDPEPGWKAADAAFEEAVLEQPEIAGAIQNRFIPVKLPLDARIV
                     RDGQEIVPLQHAAFAEMRGTPGLAIVDMTDPDGPLFRQVVSVYPFRGQYITRERLAAL
                     LHLPRGTLTQRTLIFAVRTHPEHPASALSHLSAILARECESHAFHQASITLQGHHNWE
                     SRFHKINAQLPAGMVTQEVCAESWTGQSLVEAAGECVHSWRQSPGHWEAVSSRHTLFA
                     YDMKLGANGVWYATGIFARPY"
     gene            1842..>3279
                     /locus_tag="VFV87_09285"
     CDS             1842..>3279
                     /locus_tag="VFV87_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103990.1"
                     /translation="MQKGLAVVTFLSLMLLGAASARAEEPQAKVEAALNELHQWVGEG
                     ATGEGWRKFLKSDALAAELAKGAEADPKVVGEVLAQYQSGKPGLEMSRFAAVREALAA
                     WEAELLKPTAAELPKLVRDAAGSYQPVKPEAVTQAKAELTAAVSDLDRYLSRDPKGVA
                     GWKKFLRWDELTPIVQAQEPPPTEAVAAAVDRFRANQKGLELPQFTRVRIALENYAAA
                     AVAAANGGLQEQFAQRLEALAKQLETYSQDPAAGDEAIAIGRTLGWLERNHQSPELVT
                     AVRMVYSQPNLYAYASKRLAAVGIEEEIDENQPVRDNILGTSIYGTARMKGHTKLELL
                     DSPSAALFNVLLTGQASSNNVGYNSGVSIYTTGLTQISGIKQLKMDVNGMTAGAAAAS
                     CQTATSINNIGARGRMVERIAWNRATGAQGQAQAIASNHAEARVANQMNERSAKLVAE
                     QNDRYFNKFKNPLVRRGHFPRDLNFSSRP"
BASE COUNT          631 a         1038 c         1032 g          578 t
ORIGIN      
        1 gctccgcagc cgcgtgagct gatcggctgt gaggagtttc tggaccgccg ctcgctcctc
       61 ggtagaaatg cgggagcgct ctttctcgac gtcgtcggct ggcttttcgt cggctgtctt
      121 ttgaacttcg gcacgggcgg aatcgtgctc gtcgatgatc gcggcgagct tttcgcgctg
      181 catttcgctg agcttcaact gctgggcgac cttgtcgttg cggagcgctc gcacgcccag
      241 cacgcgcacg cgaatggcct ccaggcgggc attttgcttc ggcgtcaata ccttggacag
      301 gccggcgcgg gcctggtcaa caatcttcgc gagcgccggc tgcgccgcat cggcccgctg
      361 gttgcggagc ggaaagacct gcaggtcgag cggatcgaga acggcgtgcc actcgccgcg
      421 ctgagcgtcc gtcagcttca agtcggcgac gaccgcccgg tcgtggacga ggttccagaa
      481 cgggttgtcg tccgccaggc ttgccccagc ggtgacaaaa acgcccgacg agatcaaaag
      541 ggacacgaag cagcgcacca tactgaaccc tcccgcggcc agccattcta gcgccccacg
      601 agccttagac gccagttgcc gtgcggaggt tacgttctcc gtaggacggg tttccaaccc
      661 gtctgcttgg gtcgggttgg aaacccgtct ttcgagcgtg cggaggttac gctagtacgg
      721 cctggcgaag ataccagtcg cataccacac gccgttggcc cccagcttca tgtcataggc
      781 gaagagcgtg tgccgggaac ttaccgcttc ccagtgcccg ggcgactgcc gccaactgtg
      841 gacgcactcg ccggccgctt cgaccagcga ttgccccgtc cagctttcgg cgcagacttc
      901 ctgggtcacc atccccgccg gcaattgggc gttgatcttg tgaaatcgcg attcccagtt
      961 gtggtgcccc tggagtgtaa tgctggcctg atggaaggcg tggctctcgc attcgcgggc
     1021 caggatcgcg ctgagatggc taagggcgct ggccggatgc tcggggtgcg tgcggacggc
     1081 gaagatcagc gtccgctgcg tgagcgtacc tcgcggcaaa tgcagcaacg cggccagccg
     1141 ctcgcgcgtg atgtattgcc cgcggaaggg atagacggaa acgacctggc gaaagagcgg
     1201 cccgtccgga tcggtcatat cgacgatcgc cagccccggc gtaccccgca tctcggcaaa
     1261 agccgcatgc tggaggggca cgatctcctg cccgtcacgg acgatccgtg catcgagggg
     1321 cagcttgacc gggatgaagc ggttctgaat cgcgcccgca atttccggct gctcaagaac
     1381 tgcctcttcg aacgcggcgt cggctgcttt ccagccgggc tctggatcga cgaaccaaat
     1441 caggaccatc ttgctgctgg attcgccacg atcgagcgcg tcgcggtagt cgctcagcca
     1501 agagattggg gctggggccg gcttcgtagg tggcacgtcc accgccgcaa cgatacgcaa
     1561 accaaacaga agaaagatgc cgctggacaa ccaattggtt aacataggtc gattgggttg
     1621 attgcgttgc ctggggcgtt tgatccagta ttggcgagtc aagataggcg gctatttcca
     1681 atcgcgtcaa gtagcggggt cgcgtcgatc gttcggcttg cacaattcgg gaattgcaca
     1741 gaagatgggg ccctgtttca gatgtaccgt ccatgagcct gttatcttga gatcaatcac
     1801 cagcggcgga cttacaattt ctctttggac ggagtttgga catgcaaaag ggattagcag
     1861 tcgtcacatt cctgagcttg atgctgctgg gcgcggcgag cgctcgcgcg gaggagccgc
     1921 aggccaaggt tgaggccgca ctgaacgaat tgcaccaatg ggtaggcgaa ggcgccaccg
     1981 gcgaaggttg gcgaaagttc ctcaaatcag atgcactcgc ggcggaattg gccaagggcg
     2041 cagaggccga tcccaaagtg gtcggcgagg tgctcgctca atatcaaagc ggcaagccgg
     2101 gcctcgaaat gtcccgcttt gccgccgttc gcgaggctct tgccgcgtgg gaagcggaac
     2161 tgctcaagcc gaccgcggcg gaattgccaa agctcgtgcg cgatgcagcc ggcagctatc
     2221 aacccgtgaa gcccgaggcc gtcacgcagg cgaaagccga gctgacggct gccgtcagcg
     2281 acctcgatcg ctacctcagc cgcgatccga aaggcgtggc gggctggaag aaattcctgc
     2341 gctgggacga gttgacgccc atcgttcagg cccaggagcc gccacccact gaggccgttg
     2401 ccgccgcggt cgatcgcttc cgggccaatc agaagggcct ggagctgccg cagttcacgc
     2461 gcgtccgcat cgcgctggag aactatgccg ccgccgccgt cgccgccgcg aatggcggcc
     2521 tgcaagagca gttcgcccag cggctcgaag cgctggccaa acagctcgaa acgtactctc
     2581 aagacccggc cgccggcgac gaagccatcg cgatcggccg tacgctcggc tggctcgaac
     2641 gaaaccacca gtcgccggaa ctggttactg ccgttcgcat ggtctattcg cagccgaacc
     2701 tgtatgccta tgcttccaag cgtctggccg ccgtcggcat tgaagaagag atcgacgaaa
     2761 accaaccggt ccgcgacaac attctcggca cgagcatcta cggcacggcc cgcatgaagg
     2821 gccatacgaa actcgaattg ctcgatagcc cttcggccgc gctgtttaac gtcctgctca
     2881 ccggccaggc gtcgtcgaac aacgtcggct acaacagcgg cgtctcgatc tataccaccg
     2941 gtctgacgca gatttccggc atcaaacaac tgaagatgga cgtcaacggc atgaccgccg
     3001 gcgccgctgc cgcctcgtgc cagacggcga cgagcatcaa caacatcggc gcccgcggcc
     3061 gcatggtcga gcgcatcgcg tggaaccgcg cgaccggggc gcagggccag gctcaggcca
     3121 tcgcctcgaa ccatgccgaa gcgcgcgtcg ccaaccagat gaacgagcgg tcggcaaagc
     3181 tcgtcgccga gcagaacgac cggtacttca acaagttcaa aaacccgctc gtccgccgcg
     3241 gccacttccc ccgcgatctg aacttcagca gccgccctg
//
LOCUS       DASUGB010000484         6225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1304994, whole genome shotgun sequence.
ACCESSION   DASUGB010000484 DASUGB010000000
VERSION     DASUGB010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304994"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFV87_09290"
     CDS             complement(<1..277)
                     /locus_tag="VFV87_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103991.1"
                     /translation="MTQAVRERLLGWQHAFDHPFTLWATIIIVGLAVASPLVILVLRA
                     AGRLSDKAYTELLARCQTWAVLVPLLFAPILAGAFWTILGVAALSFLC"
     gene            complement(274..957)
                     /locus_tag="VFV87_09295"
     CDS             complement(274..957)
                     /locus_tag="VFV87_09295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5103992.1"
                     /translation="MNEQGYDPSDRRPIAARELAISRRLANRVADLGASPNGISIAGM
                     IAALVGGAAFAATAQGSPYVRLFWLAAAVLTQFRLLANMLDGMVAIRRNLASPLGELY
                     NEVPDRISDAALLIGVGFAAGGNPLLGFGAALAAVFTAYVRSMAKAAGAPNDFCGPMA
                     KQQRMFTITLVALYCAAAPLAWQPMGTLGRETFGLASAALAIVLVGSVFTALRRLVRA
                     ARHLKAKHL"
     gene            complement(950..2044)
                     /gene="mtnA"
                     /locus_tag="VFV87_09300"
     CDS             complement(950..2044)
                     /gene="mtnA"
                     /locus_tag="VFV87_09300"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5103993.1"
                     /translation="MPTKIETLRWIGDENGHLVLIDQTRLPTELVQRECRDVETVWEA
                     ITQLRVRGAPAIGIAAAYGVVLGLQEGAFRSAHAPYGNEAAFYERLAEVTKYLAESRP
                     TAVNLFWALDRMKAKAAALRGTGSTVDIHRALSAEAQAIHAEDREICHAIGRFGAELL
                     SDQAGVLTHCNAGGLATAEYGTALSVLFTCQDQGKKLHVFVDETRPLLQGARLTAWEL
                     AQRGIEATLICDNMAGQVMREGKVQAVIVGADRIAANGDTANKIGTYSVAVLAQAHGI
                     PFYVAAPTTTFDLSIVSGDQIPIEQRAGQEITHGFGRQTAPEGIGVYNPAFDVTPARL
                     VKAIITERGVIEPVSRETIANIVGRGPRRE"
     gene            complement(2129..3232)
                     /gene="ald"
                     /locus_tag="VFV87_09305"
     CDS             complement(2129..3232)
                     /gene="ald"
                     /locus_tag="VFV87_09305"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5103994.1"
                     /translation="MLIGVPREIKRDEYRVAMLPVGIEELKRAGHQVLIETSAGLGSG
                     IPDSEYAAEGAEVVATAKEIYQRADLIVKVKEPLPAEYPLIKSGQAIFTYFHFAASRE
                     LTEAMLASGASCLAYETLKDAQGRLPLLTPMSEVAGRMSIQEGAKYLERPQMGRGILL
                     AGVPGVAPAHITILGGGVVGANAAKIAAGFQADIAILDVNMDRLRYLDDIMPPNVNVL
                     YSDRHTIRAQLKLADLVIGAVLIPGARAPYLISQADLKHMKPGSVIIDVAIDQGGCVE
                     TSRPTTHSDPTYLEEGVLHYCVTNMPGAVGRTSTFALCNVTLPWVLQIAKKGVEPAIA
                     QSAPLASALNIHHGRVTNQAVAETFGLKHEPLV"
     gene            complement(3253..3567)
                     /locus_tag="VFV87_09310"
     CDS             complement(3253..3567)
                     /locus_tag="VFV87_09310"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5103995.1"
                     /translation="MKVHWTERARRNLRAIHDYIAEDSPNYAIRMVDRITRKTESLAR
                     FPMLGHVVPEYEAEQGTEPTVRQILEGNYRVIYRLRPDRVQVLTVIHGARQLPPLEAL
                     ES"
     gene            complement(3564..3752)
                     /locus_tag="VFV87_09315"
     CDS             complement(3564..3752)
                     /locus_tag="VFV87_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5103996.1"
                     /translation="MADISVKQEARKIVEGLPEDATWEDLMYRIYVREAIDAGIRDAD
                     EGRVVDVSQVRAEYGLPS"
     gene            complement(3800..5239)
                     /gene="der"
                     /locus_tag="VFV87_09320"
     CDS             complement(3800..5239)
                     /gene="der"
                     /locus_tag="VFV87_09320"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5103997.1"
                     /translation="MAVPQVVIVGRPNVGKSSLLNWLAGKRISIVDDQAGVTRDRVTH
                     LMEHNGRYFELVDTGGIGIADVDNLTKEIEQQIDAALDAADVILFVVDTRTGTVSLDN
                     DIAKRLRYIEKPIVCVANKTDDERMEPQADEFYKFGLKLLKVSTLQNRGKDDLLGEIY
                     RKIPRNVMFEKLPEEPAMKVAIVGRRNVGKSTFVNTLAKAERMIVSEVPGTTRDSVDV
                     QFELDGKTFVAIDTAGVRRHKSIRTDIDFYSFHRAQRSIRRADIVLLFFDCTQRLSKV
                     DKQLCKYIADQYKACIFVVNKWDLLYGQMPTERWVDYLRDSFPTMWHVPIAFITGQTG
                     KNVKALLNHAQMLFKQSLQRVATSELNRLVRAALTKHPPPMHGILRPKIYYATQVSIQ
                     PPTMVLICNEPKAFQPNYRRYLLSILRDNLSFGEVPIKMYLQKRRPSDKRDDLEAGED
                     TPAELAAMEVEFGEEEDEEDTQLEDSEIE"
     gene            5357..>6225
                     /locus_tag="VFV87_09325"
     CDS             5357..>6225
                     /locus_tag="VFV87_09325"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5103998.1"
                     /translation="MNADRESTDRARRMLTQRLQSLQRSGVQQIGRVPAPTPTRRASE
                     GAPISATPTRRASEGVPTVTRRVSEGISPTAPPQDPPMPRTKAAAPAAAAGSSMLSLI
                     GQPYPADSPNDAAGCQSLLDALNAEVRPCQLCRELAATRKQTVFGVGNPRPRVVFFGE
                     APGADEDRQGEPFVGRAGQLLTKIIEACGWQRGDVYIMNVLKCRPPENRNPSPQETDN
                     CRPYFERQFEILRPEYIVCVGTVPAQALLETTESVGKLRSRFHRYRDSQVLVTYHPSY
                     LLRNPSAKKYVWE"
BASE COUNT         1096 a         2038 c         1942 g         1149 t
ORIGIN      
        1 agcacaggaa gctgagggcc gcgacgccca ggattgtcca gaaggcgccg gccagaatcg
       61 gcgcaaagag cagcggcacg aggacggccc aagtctggca gcgggcgagc aactccgtat
      121 aagccttgtc gctcaagcgg ccggcggctc gcaaaaccag gatcaccagc ggcgaagcaa
      181 ccgccaggcc gacgatgatg atcgtcgccc acagcgtgaa ggggtggtcg aaggcgtgtt
      241 gccagccgag gaggcgctcg cggacggcct gcgtcataaa tgtttggcct tgagatgccg
      301 ggccgcgcgg accagccggc ggagggccgt gaagaccgag ccgacgagga cgatggcgag
      361 tgccgcggag gccaagccga acgtctcgcg gccaagcgtc cccatcggct gccaggcgag
      421 cggcgcggcg gcgcaataga gcgccacgag cgtaatcgta aacatccgct gctgcttggc
      481 cattgggccg cagaagtcat tcggcgcgcc ggcggctttg gccatcgaac ggacgtacgc
      541 cgtgaacacc gccgccaaag ccgccccaaa accgagcagc gggttgccgc ccgcggcgaa
      601 gccgacgccg atcaagagag cggcatccga gatgcgatcg ggcacttcat tgtaaagctc
      661 gcccagcggc gacgcgagat tgcggcggat ggcaaccatc ccgtccagca tgttcgccaa
      721 gagccgaaac tgcgtcagca ccgcggcggc cagccaaaac aggcggacat acggcgagcc
      781 ctgcgccgtg gcggcaaacg cagcgccgcc gacgagcgcc gcaatcattc cggcgatcga
      841 aatcccgttg ggcgaagcgc ccaggtcggc gacccgattc gccagccgcc gcgagatcgc
      901 cagctcgcgg gccgcgatgg gccggcggtc ggatgggtcg tacccctgtt cattcacgcc
      961 gaggccctcg ccccactatg ttcgcaatcg tctcgcggct gacgggttcg atcacgccgc
     1021 gctcggtgat gatggctttg acgagccggg cgggcgtgac gtcgaaggcc ggattataga
     1081 cgccgatccc ctccggcgcg gtctgcctgc caaagccgtg cgtgatttcc tggccggccc
     1141 gctgctcgat ggggatttgg tcgccggaaa cgatggagag gtcgaacgtg gtcgtcggcg
     1201 cggcaacgta aaacgggatt ccgtgggctt gagcgaggac ggccacactg tacgtgccga
     1261 tcttgttcgc cgtgtcgccg ttagcggcaa tccggtcggc gccgacgatc acggcctgca
     1321 ctttgccttc gcgcatcacc tggcccgcca tgttgtcgca gatcagcgtc gcctcgatcc
     1381 cgcgctgcgc gagctcccac gccgtcaatc tcgcgccttg caggagtggg cgggtctcgt
     1441 cgacaaagac gtgcagcttc ttgccctgat cctggcaggt gaacagcacc gacagggccg
     1501 tgccgtattc tgccgttgcc aggccaccgg cgttgcaatg cgtcaggacg cctgcctgat
     1561 cggataacag ctcagcgccg aagcggccaa tcgcgtggca gatttcgcgg tcctcggcgt
     1621 gaatggcctg cgcttcggcc gagagcgccc ggtggatgtc cacggtcgat ccggtgccgc
     1681 gaagcgccgc tgccttggcc ttcatccggt cgagcgccca gaacaggttc acggctgtcg
     1741 gccggctttc tgcgaggtac ttcgtgactt cggccagccg ctcgtagaac gccgcctcat
     1801 tcccgtaggg tgcatgagcg gagcgaaagg cgccttcctg caggccgagc accacgccgt
     1861 acgcagccgc gatgccaatc gccggcgcgc cgcggacgcg aagctgcgtg atggcctccc
     1921 agacggtctc tacgtcgcgg cactcgcgct ggaccagttc cgtcggcagg cgcgtctggt
     1981 cgatcaggac cagatggcca ttctcgtcgc cgatccagcg gagagtttcg attttggttg
     2041 gcatccacct agtttatcgt cgttttgtgc ggagccgaag gcgacgcatc gcacaatgct
     2101 ttgccacggc atcgcctgcg gctcgccgtt aaacgagagg ctcgtgcttc aggccgaagg
     2161 tttccgccac tgcctgattg gtgactcggc cgtggtggat gttgagggcg gaggcgagcg
     2221 gggcggattg ggcaatggcc ggctcgaccc ccttctttgc gatctgcagg acccagggga
     2281 gcgtgacgtt gcagagggcg aacgtgcttg tgcggccgac cgcaccgggc atgttcgtga
     2341 cgcagtagtg cagcacgcct tcctccaggt acgtcgggtc gctgtgcgtc gtcggccggc
     2401 tcgtctcgac gcagccgccc tgatcgatgg cgacgtcgat gatcacgctc cccggcttca
     2461 tgtgcttgag gtcggcctgg gaaatgagat agggggcgcg ggcgccggga atcagcacgg
     2521 ccccgatcac caggtcggcc agcttgagct gcgcgcggat cgtatgccgg tcgctgtaga
     2581 ggacgttgac gttgggcggc atgatgtcgt cgagatacct cagccggtcc atgtttacgt
     2641 cgaggatcgc gatatccgcc tgaaagccgg cagcgatctt ggcggcattc gcaccaacca
     2701 cgccgccgcc gaggatcgtg atgtgagccg gagcgacacc cggcacgccg gccagcagga
     2761 tgccacggcc catctgcggc cgctccagat acttggctcc ctcctggatg ctcattcgcc
     2821 cggccacttc gctcatcggc gtcaagagcg gcagccggcc ctgggcgtcc ttgagcgtct
     2881 cgtaggccag acagctcgcg ccgctggcga gcatcgcttc ggtcagctcg cggctggcgg
     2941 cgaagtgaaa ataggtgaaa atcgcctggc cgctcttgat cagcggatac tctgccggca
     3001 gcggctcttt aaccttcacg atcagatcgg ctcgctgata gatttcctta gccgtggcga
     3061 cgacttcggc cccttccgcg gcatattcgg agtccggaat gcccgaacct agtccagcgc
     3121 tggtctcgat caggacctgg tgcccggccc gcttcagctc ctcgatcccc accggcagca
     3181 tcgccacgcg atattcatcc cgtttgatct cccgcggcac gccgatgagc atgggcaatc
     3241 ctttcctacg gatcacgatt cgagcgcttc caacggcgga agctgccgag caccatgaat
     3301 gaccgtcagc acttgtacgc ggtccggccg cagtcgatag atcacgcggt aattgccttc
     3361 caggatttgc cggacagtcg gttcggtgcc ctgttctgct tcatattccg gaacgacgtg
     3421 gcccaacatt gggaaacggg ccaggctctc tgttttgcgc gtgattcgat cgaccattcg
     3481 aatcgcgtaa ttcggagaat cttcagcgat gtagtcgtga atggctcgca ggtttcgccg
     3541 cgctcgttct gtccagtgaa ccttcacgat ggcagaccat actcggcgcg gacctggctg
     3601 acatccacga cacgcccttc gtcagcgtcg cgaattcccg cgtcaatcgc ttcgcggacg
     3661 tagatccggt acatgaggtc ttcccacgtg gcatcctcgg ggagaccctc cacgatcttg
     3721 cgagcctctt gtttgacgga aatatcggcc atgctgcggc tcctgcattc aaacggtcac
     3781 tcgttccctc cacttcatct tactcgatct cactgtcttc aagctgagta tcctcctcgt
     3841 cttcctcctc gccgaactcg acttccatcg cggccagttc ggcgggggta tcttcgccgg
     3901 cttccaggtc gtcgcgcttg tcgctcggcc ggcgcttctg caagtacatc ttgattggca
     3961 cttcgccgaa actcaggtta tcgcggagga tgctgagcag gtaccggcgg taattcggct
     4021 gaaaggcctt gggctcgttg cagatcagca ccatcgtcgg cggctggatg ctgacctgcg
     4081 tggcatagta gatcttgggc cgcaatatgc cgtgcatcgg cggcgggtgc ttcgtgagcg
     4141 cggcccgcac cagccgattg agctcggaag tcgccacccg ctgcaggctc tgcttgaaga
     4201 gcatctgggc gtgattcagc agggccttga cgttcttgcc cgtctggccc gtgatgaacg
     4261 cgatcggcac gtgccacatg gtggggaacg aatcgcggag gtaatcgacc caccgctccg
     4321 tcggcatctg gccgtacagc aggtcccact tgttgacaac gaagatgcac gccttgtatt
     4381 gatcggcgat gtatttgcaa agctgcttat cgactttcga caatcgctgc gtgcaatcga
     4441 agaagagcag cacgatatcg gcccggcgga tgctccgctg ggcgcggtga aagctgtaaa
     4501 agtcgatatc ggtccggatg ctcttgtgcc gccgcacgcc ggccgtgtcg atcgccacga
     4561 acgttttgcc gtccagctcg aactgcacat cgacgctgtc ccgcgtcgtg ccgggaacct
     4621 cgctgacgat catccgctcg gctttggcca gcgtgttgac gaacgtgctc ttgccgacgt
     4681 tccgccgccc gacaatcgcc accttcatcg ccggctcttc cggcagcttc tcgaacatca
     4741 cgttccgcgg gattttgcgg taaatctcgc ccagcagatc gtccttgccg cggttctgca
     4801 gcgtgctcac cttcagcagc ttcaggccga atttgtagaa ctcgtcggcc tgcggctcca
     4861 tgcgctcgtc gtcggtcttg ttggccacac agacgatcgg cttctcgatg taccgcaggc
     4921 gcttggcgat gtcgttatcc agcgacacgg tgccggtgcg ggtatcgacg acgaacagga
     4981 tcacatccgc ggcatcgaga gcggcatcga tctgctgctc gatctccttg gtcagattgt
     5041 caacgtcggc gatgccgatg ccccccgtat cgaccagctc gaagtaccgg ccgttgtgct
     5101 ccatcaggtg cgtgacgcgg tcgcgggtga cgcccgcctg atcatcgacg atcgaaatcc
     5161 gcttgcccgc cagccaattg aggaggctcg atttccccac attcgggcgg ccgacgatga
     5221 cgacctgcgg aacggccatg gaaccaagaa accggctgtg gcgggcactg ggacgagcaa
     5281 gtgggcgaga attgtatgat agtcgaaccc ccgcccatcg gcagggggtc ggttttccgt
     5341 taagaattgg tcaccgatga atgcagatcg cgaaagcacg gacagggctc gccggatgct
     5401 cacgcagcgc ctccagagcc tgcagcggtc cggcgtccag caaatcggca gggttcccgc
     5461 acccacacca acccgacgtg ccagcgaggg agctccaatc tccgccacac caacccgacg
     5521 cgccagcgag ggagtgccca cagtaacccg acgcgtaagc gagggcatct ctcctactgc
     5581 cccgccccag gatcctccaa tgccacgcac taaagccgct gcgcccgccg ctgcggcggg
     5641 ctcttcgatg ctgtcgctca tcggccagcc ttatcccgcg gattcgccga acgacgccgc
     5701 gggctgccag tcgctgttgg acgctctcaa tgccgaagtc cgcccgtgcc agctttgccg
     5761 cgaacttgcg gcaacccgca aacagacggt cttcggcgtc ggcaacccac ggccacgcgt
     5821 ggtcttcttt ggcgaagcgc ccggcgccga cgaagaccgc cagggcgagc ccttcgtcgg
     5881 tcgagcgggg caattgctga cgaagatcat cgaagcctgc ggctggcagc gtggcgatgt
     5941 ctatatcatg aacgtcctca aatgccgccc gccggagaac cgcaacccct cgccgcaaga
     6001 gacggacaat tgccggccgt acttcgagcg gcaattcgag atcctgcggc cggaatacat
     6061 cgtctgcgtc ggcaccgtcc cggcccaggc cctgctggaa accacggaat cggtcggcaa
     6121 gctccgcagc cgcttccacc gctaccgcga tagccaggtc ctcgtcacct accacccgtc
     6181 ctatctgctc cgcaatccct ccgccaagaa atacgtctgg gaaga
//
LOCUS       DASUGB010000485         4800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_55065,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000485 DASUGB010000000
VERSION     DASUGB010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55065"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFV87_09330"
     CDS             complement(<1..1147)
                     /locus_tag="VFV87_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEX5103999.1"
                     /translation="MSLLFRVVFHTACQSTHHRLALDALRHLRGADSERWNDLFLHHF
                     RGYLSGSKAPDDQFKDFRNHVLHVSENNWGGAIPEASRWYGRTVDALRRREWSEAVYS
                     AGVLSHYFSDPFMPLHTGQTEAETQVHRALEWSVSKSYGELQQIIEFELGGYPRIESS
                     SSADWLGQMILAGAQLAHEHYQPVIDHYDLARGVKNPQAGMDGECKHRIAECLAHAVV
                     GFARVLEKAISESDIEPPQVENTLYGFLVALEAPLRVVLSHFHDLTERMTIEAIYDEV
                     QRTGKVIKNLPEDDRDIRRLHAEEVLRTPLHRLDQQPAPLAGMLFGQGQEDRQHPNRL
                     VMAPVVGEDLSRPLALVARRQGTGDRGQRTGDRGQRTEDRGQGTEDRG"
     gene            complement(1245..2471)
                     /locus_tag="VFV87_09335"
     CDS             complement(1245..2471)
                     /locus_tag="VFV87_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104000.1"
                     /translation="MRLLCSLAAVFTLCVPFVAQTAEPNWPRWRGPQQDGHSAETDLP
                     VKWTGENIVWKTDLPGIGQSSPVIWGDRIFLTAGLDKGAERIVLCVDRKTGKILWQQT
                     AWKGAPETVHAMNCWASATCVTDGELVVAFFGRGGIHAYSAAGEHLWSRDLGRFEGPW
                     GTAACPILVDDLVIQNCDADVDAFLIALNKRTGEEVWRTKRPDHRGWSTPIVVQAAQR
                     RELVLNGDEGVQAYDPATGQELWFCKGFNGRGEPTVTSAGDLLCVVNGKAGDFYSVRP
                     GGSGDVTESHMAWHTPRKGGRDCPSPIVVGRYIVVCDMGGIATCYDAEDGHIYWKERL
                     PGKYSGSPIAANGLAYFLNEDGKTVVIEPGEALKIVAENDLPARKDEIFRASPVPLAG
                     QLFLRSTSVLYCLGNK"
     gene            2637..4460
                     /locus_tag="VFV87_09340"
     CDS             2637..4460
                     /locus_tag="VFV87_09340"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5104001.1"
                     /translation="MNQQDVRLRPANSWRKDAKRLLGVDRLVLQIHDASFPSDPDEDL
                     GRGSPYTRGAERFLVWAAGLGFDAIQFGPRGMTSRGNPSPYDATIFSRNPLDLPLLKL
                     VEQGRVSRVTWESIRRSFPPATDGVVPYPLVFAAYEQALAEIAATADRSDRIAAREFL
                     AANVAWLAPDALYGQLSREHGSHSWLQWSRTPRGALDQGLLDPPPGCERSAAKRLAEL
                     RHQHARVIENYALIQWLLVEEHRQLRERMARVGLLLFGDLQVGLSFQDTWARQRAFLR
                     GYRMGAPPSRTNPQGQPWGYAVLDPAQFGTAEEPGPALAFVRARIARVLAECDGLRID
                     HPHGWIDPWVYRSEDPDPLHAVQTGARLFSSPNDPQHPALAKLAIARPDQIDHAELHY
                     ADGRVKDLDDDQVRRYSILVDEIVTQQAAAGRAKAIACEVLSTLPYPVGRVMERHDLG
                     RFRVTQKLDLGNPADVYRLENALPHDWIMLGTHDTPPIWQLAEQWQGGERAAGFGRYL
                     AELLAAPAEREALAAQIAGNSGRLVNSLFAAMLASRARNIVVFFPDLFGLTVRYNEPG
                     VVSDANWSLRVPADFEAFYDRQRQRGRALDLDACEELATNK"
     gene            complement(4468..>4800)
                     /locus_tag="VFV87_09345"
     CDS             complement(4468..>4800)
                     /locus_tag="VFV87_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104002.1"
                     /translation="GGQYVPLQTAGPLAQHLIALGWRRGEHQPLELVAAIPRHVQALL
                     AARAPTDRSAPLWVDGAWAETQVLLPDGSPSNWRCLLTERTHSTADNRLSIPDLLSDF
                     PLAVLCSV"
BASE COUNT          786 a         1504 c         1637 g          873 t
ORIGIN      
        1 gtcctctgtc ttctgtcccc tgtcctctgt cttctgtcct ctgtcccctg tctcctgtcc
       61 tctgtcccct gtctcctgtc ccctgtctcc tcgcaaccag ggccagcggc cggctcaggt
      121 cttctccgac gaccggcgcc atcaccaacc ggttgggatg ttgccggtct tcctggcctt
      181 ggccgaagag catgccggcc aggggggcgg gttgctggtc gaggcggtgg agcggcgtcc
      241 tcaggacctc ttcggcgtgc agccggcgga tgtcccggtc gtcttcgggc aggtttttga
      301 tcaccttgcc ggtccgctgg acttcgtcgt agatcgcctc gatcgtcatc cgttccgtca
      361 ggtcgtggaa gtggctcagc accacgcgga gcggcgcttc gagggcgacc aggaagccgt
      421 agagcgtgtt ctcgacctgc ggcggctcaa tatcggattc cgatatcgcc ttttcgagca
      481 cgcgggcgaa gccgacgacc gcgtgggcca ggcactcggc gatgcggtgt ttgcactcgc
      541 cgtccatccc ggcctgcggg ttcttcacgc cgcgggccag gtcgtagtgg tcaatcaccg
      601 gctgatagtg ctcgtgggcg agctgcgcgc cggccaggat catctgccca agccaatcgg
      661 ccgagctgct cgattcgatc cgcggatagc cgcccagctc gaattcgatg atctgctgca
      721 gctcgccata cgacttcgac acgctccatt cgagggcccg gtgtacctgc gtctcggctt
      781 cggtctggcc ggtgtgcagc ggcatgaacg ggtcggagaa gtaatggctg agcacgccgg
      841 cggaatagac cgcttcggac cactcgcggc ggcggagcgc atcgaccgtg cggccgtacc
      901 agcggcttgc ctccggaatg gcaccgcccc agttgttctc gctcacatgc aggacgtggt
      961 tgcgaaagtc cttgaactga tcgtccggtg ccttgctccc gctcaggtag ccgcggaagt
     1021 ggtgcaggaa caaatcgttc caacgttcgc tgtccgcccc gcgcagatgg cgcagcgcat
     1081 cgagcgcaag gcgatggtgg gtgctctggc acgcggtgtg gaagacgacg cgaaagagga
     1141 gcgacacggc cggcgtcctt gctagcagtc gttaggtcag gcgggcctga cttgtagcag
     1201 aatcccggcc gctagcaaag acgaacgcgt gatccgccgc gtgctcactt gtttcccagg
     1261 caatacagca cgctcgtgct gcggaggaat agctggccgg ccagcgggac aggggaggcg
     1321 cggaagattt cgtccttccg ggcggggagg tcgttttcgg cgacgatttt aagcgcttcg
     1381 ccgggctcga tgacgacggt cttgccgtct tcgttgagga aataggccag cccattggct
     1441 gcgatgggcg agccgctgta tttgccaggg aggcgctctt tccaatagat gtggccgtcc
     1501 tcggcgtcgt agcaggtggc gatgcctccc atgtcgcaaa cgacgatgta ccggccgacg
     1561 acgatggggg aggggcaatc gcggccgccc ttgcggggtg tgtgccaggc catgtgcgat
     1621 tcggtcacgt cgccgctgcc gccgggacgc acggaataga aatcgccggc cttgccgttg
     1681 accacgcaga gcaagtctcc ggccgacgta acggtcggct cgccgcgtcc gttgaagccc
     1741 ttgcagaacc acagttcctg acccgtggcc ggatcgtagg cctgcacgcc ttcgtcgcca
     1801 ttgaggacca gttcgcgccg ctgggcggcc tgaacgacga tcggcgtgct ccagccacga
     1861 tggtcggggc gcttcgtgcg ccagacttcc tctccggtgc gcttattcag ggcgatgaga
     1921 aaggcatcga cgtcggcgtc gcagttctga atgaccagat catcgaccag aatcgggcag
     1981 gccgcggttc cccaggggcc ctcgaagcgg cccaggtcgc gcgaccagag gtgctcgccc
     2041 gctgccgaat aggcgtggat gccgccgcgg ccaaagaagg cgacgacgag ttccccgtcg
     2101 gtcacgcacg tggcggaagc ccagcaattc atggcgtgga ctgtctcggg cgcgcccttc
     2161 caggccgttt gctgccagag gattttgccg gtctttcgat cgacgcacag gacgatacgc
     2221 tctgcacctt tgtcgagacc tgccgtgaga aaaatccggt cgccccagat gaccggcgac
     2281 gactggccga tgccgggaag gtcagttttc caaacgatgt tctcacccgt ccatttcacc
     2341 ggcaggtccg tttcggccga gtgtccgtct tgctgcggac cgcgccagcg gggccaattg
     2401 ggctcggccg tttgagctac gaatggtacg cacaacgtga atacagcggc gagcgagcag
     2461 aggaggcgca tggggggatc cttcgggcgg gagtctggca ggttgggatt catggtaggc
     2521 aactggccat gcccaacgca acggctgggt cgattgaccg attgaaagtt ctctaggcaa
     2581 ttcagaagct acatttcaaa atgaggcctc aacgaacagg agaaacgatc ggcgcgatga
     2641 atcagcagga tgtgcggctg cggccggcca attcatggag gaaagacgcg aagcgcttgc
     2701 tgggcgtgga tcggctggtg ctgcagattc acgacgcgag ctttccgtcc gatccggacg
     2761 aggaccttgg ccgcggcagc ccgtacacgc ggggggcgga gcgattcctg gtatgggccg
     2821 ccgggctggg attcgatgcg attcaattcg ggccgcgcgg gatgaccagc cgcggcaatc
     2881 cgtcgcccta tgacgcgacg attttctcgc ggaatccgct cgatctgccg ctgctgaagc
     2941 tggtcgaaca gggccgagtg agccgggtaa catgggagtc gatccgccgc tcgtttccgc
     3001 cggcgacgga tggcgtggtg ccgtatccgc tcgtttttgc ggcgtacgag caggccctgg
     3061 cggaaatcgc ggccacggcc gaccgctccg atcggattgc tgcccgcgaa tttctggcgg
     3121 cgaatgtcgc gtggctggcg cccgatgcgc tctacgggca actcagccgc gagcacggca
     3181 gccattcgtg gctgcagtgg agtcgcacgc ctcgaggagc gctcgaccag ggcctgctcg
     3241 atccgccgcc gggctgcgag cggtccgcgg cgaagcggct ggcagagctg cgccaccagc
     3301 acgcgcgagt gatcgagaat tatgccctca tccagtggct gctcgtggag gagcaccggc
     3361 agttgcggga gcggatggcc cgggtggggc tgttgctgtt tggcgatttg caggtcgggc
     3421 tctcgtttca agacacttgg gcccggcagc gggcgttcct ccgcggctat cggatgggtg
     3481 cgccccccag ccgcaccaat ccgcaaggcc agccgtgggg ctatgcggtg ctcgatccgg
     3541 cacagttcgg cacagcggag gaacctgggc cggcgttggc ctttgtgcga gcgcggatcg
     3601 cgcgggtcct ggccgaatgc gacgggctgc ggatcgacca tccgcacggc tggatcgatc
     3661 cgtgggtcta tcgatcggaa gaccccgacc cgctgcacgc cgtgcagacc ggggcacggc
     3721 tcttttcctc gcccaacgat ccgcagcatc ccgcgctggc gaagctcgcg attgcccgcc
     3781 cggaccagat cgatcacgcc gagctgcact acgctgacgg ccgcgtcaag gacctcgatg
     3841 acgaccaggt gcggaggtac tcgatcctcg tggatgagat tgtcacgcag caggccgctg
     3901 ccggtcgcgc gaaggcgatt gcctgtgagg tgctgagcac attgccgtat ccggtcggac
     3961 gggtcatgga gcggcacgac ctgggccgct ttcgcgtgac gcaaaagctc gatctgggca
     4021 atcccgccga tgtgtatcgc ctcgaaaacg ccctgccgca tgactggatc atgctcggca
     4081 cgcacgacac gccgccgatc tggcaactgg ccgagcagtg gcagggtggc gagcgagccg
     4141 ctggctttgg tcggtatctg gcggaactgc tcgcagcgcc ggcggaacgc gaagcgcttg
     4201 ccgctcagat cgccggcaac tcgggccgac tcgtgaactc cctgttcgct gccatgcttg
     4261 ccagccgagc acggaacatc gtcgtctttt ttccagacct gtttggtctg accgtccgtt
     4321 acaacgagcc gggcgtcgtg agcgacgcca actggtcgct ccgcgtgccg gctgatttcg
     4381 aagcgttcta cgaccggcag cgtcagcgcg gccgggcgct tgatctggac gcgtgcgagg
     4441 aactggccac gaacaagtag ctgggagtca caccgagcac agcacggcca gcgggaagtc
     4501 agacaggaga tccgggatgg agaggcgatt gtcggcggta gaatgggttc gctcggtcag
     4561 caggcagcgc cagttgctag gcgagccgtc cggcaggagc acctgcgtct cggcccacgc
     4621 gccatcaacc cacagcggag cggaacgatc ggtcggagcg cgcgccgcca acagcgcctg
     4681 gacgtgtcgc gggatggcgg cgacgagttc cagcggctga tgctcgccgc gccgccagcc
     4741 gagcgcgatc aggtgctgtg ccagcggtcc ggcggtttgg agcggcacgt attgcccgcc
//
LOCUS       DASUGB010000486         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1305462, whole genome shotgun sequence.
ACCESSION   DASUGB010000486 DASUGB010000000
VERSION     DASUGB010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305462"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFV87_09350"
     CDS             complement(<1..497)
                     /locus_tag="VFV87_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104003.1"
                     /translation="MTLDLSLNYLPQLPQDKSPPIGCIGSGFIMADCHLVAYRKAGFN
                     PVAIASRTPAHARRVAERHGLTAYDTYQEMLASGKVEVVDIAVPPDTQIDVIREVARH
                     KKHIRGILAQKPLGVSYEQAKEIVQLCKRAKITLAVNQNMRYDQSVRACKDLLSRGLL
                     GEPVLA"
     gene            629..2062
                     /locus_tag="VFV87_09355"
     CDS             629..2062
                     /locus_tag="VFV87_09355"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5104004.1"
                     /translation="MPQPRILFVSYAHEDAPWLDRILKAFQEKADPELLKVWTDRDIG
                     TSDDWLVSILQAARQSKVALLVVSRSFHDSDFIRLRELPELLNLYQQGRIQLTWVRVD
                     GVDYRLTRYSEIQSVVAPEPPLENLPPTDQDLKLQELVDKLILLLQGQRVRLPLEGRQ
                     NQHKGWLACACIAATLAALWACDYFLAPWRPEPTEVLRLIHGSNWIGYEPANFLPDVT
                     TNVPDAEIRRELQQLRAAGFTGIVTFGSRADLARIPAIAHDEGLAVIMGVWSPLDPEE
                     LERAYEQRQYVSAYCVGHNCLSDDRGLSLCTERDLERAVHWLRRSTRKPVTTTQLGKF
                     YQADAARLNLMGDFLFPDLHHNYRYDDVQQDVEITTAEILRVSQIARQYSRPLMLKTL
                     SFPARRSAQSPPDYDRQLEYFASVVGAARDPLSGPQIPVAFVAFSAFDSPWKRGGNFQ
                     SWDPFTGWFDSSGQERPVVQQWIWLRN"
     gene            2104..>3121
                     /locus_tag="VFV87_09360"
     CDS             2104..>3121
                     /locus_tag="VFV87_09360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5104005.1"
                     /translation="MAPLMLMVAFTVFLLAPWMSRVDLHDPVLTALCLSNTAVWWIVL
                     WWSLHHLTFKVGSLFRSDRGHALPQAAASGLRMTVLYPTCNDFQWQACHSCVSQDYSA
                     AQYQVVICDDSSSESYRKRIDEFQRTFPEVIVLRRNRRQGFKAGNLNHALASAQVTGE
                     WLIIVDADQILPQHYVSQFAGFVAGLPDRVGYAQARQESDHVLPTSSSHFQQALGREI
                     DIFYSHDLALRSEFGFMPLVGHGAALRRTAWQQVPFPEVVSEDFAMSLEMRNRDLVGA
                     YAPEVQAWESFPPSFGAFLIRLRKFAGGTAELYRKHLPAFLFGPATFTEKLDLLMMIG
                     WYALM"
BASE COUNT          546 a          927 c         1002 g          646 t
ORIGIN      
        1 gccagcaccg gctcgcccaa caggccgcgg ctgaggaggt ccttgcaggc ccggaccgac
       61 tggtcgtagc gcatgttctg gttcaccgcc agcgtgatct tggcccgctt gcagagctgc
      121 acgatctcct tcgcctgctc gtagctgacg cccagcggct tctgggccag gatgccgcga
      181 atgtgctttt tgtgcctggc aacctcgcgg atcacatcga tctgcgtgtc gggaggcacg
      241 gcgatatcga ccacctccac cttgccgctg gcgagcatct cctggtacgt gtcgtacgcc
      301 gtcagtccat gccgctcggc cacgcgcctg gcatgcgccg gcgtgcgcga ggcgatcgcc
      361 accggattaa agcccgcctt gcgatacgcc accaaatggc aatcggccat gatgaatccg
      421 ctgccgatgc agccgatggg cggcgatttg tcctggggga gctgtgggag gtaattgagg
      481 gagaggtcga gggtcattgg ttgaaaaaaa gggtattgtc gcggcttgga gcttgaacga
      541 cgacagcgcg gcaactatac acgatgcata aatctgtgcg aatccatcga tcgctcccac
      601 gcgtgtgccg gtattgagga catggcggat gccgcagccg cgaatactgt ttgtcagcta
      661 tgcgcacgag gatgcgccgt ggctggaccg gatcctgaag gccttccagg agaaggctga
      721 ccccgagttg ctcaaggtgt ggacggaccg ggacatcggc acgagcgacg attggctggt
      781 ttcgattctt caagccgcgc ggcagtcgaa ggtggcgctg ctggttgttt cccggagctt
      841 tcatgactcg gattttatcc ggctccgcga gctgcccgag ctgctgaatc tctatcagca
      901 ggggcgcatt cagttgacct gggtgcgtgt ggacggcgta gattaccggc tgaccaggta
      961 cagcgaaatc cagtcggtgg tcgcccccga gccgcccctg gagaacttgc cgccgaccga
     1021 tcaggacctc aagctgcagg aactcgttga caagctgatc ctgctgctgc aagggcaacg
     1081 ggttcgtttg ccgctggaag gccgacagaa ccagcacaaa ggctggctgg cctgtgcctg
     1141 catcgcggcc acgctggcgg cgctatgggc ctgcgactat ttcctggccc cctggcgtcc
     1201 ggaaccgacg gaagtgctcc gcctgattca tgggtcgaat tggatcggct acgagcctgc
     1261 caatttcctt cccgatgtga ccaccaacgt tcccgacgcg gaaattcgcc gcgagctgca
     1321 gcaattgcga gccgctggtt tcacgggaat cgttacgttc ggcagccgcg cggatctcgc
     1381 gcgcattccg gccatcgcgc acgacgaagg cctggcagta atcatggggg tttggagccc
     1441 cctggacccg gaggaactgg agcgggccta tgagcagcgg cagtacgtat cggcctactg
     1501 cgtggggcac aactgtctga gcgacgaccg gggcctgagt ctgtgtacgg agcgcgacct
     1561 ggagcgcgcg gtccactggc tccgccggag cacgcgcaaa ccggtgacca cgacgcagct
     1621 cggcaaattc tatcaagccg acgcggctcg actgaatctg atgggagact ttctgtttcc
     1681 cgatttgcac cacaactatc gctacgacga cgtgcagcag gacgtcgaga tcacgaccgc
     1741 cgagatcctg cgtgtttcgc aaattgcccg gcagtacagc cggccgctga tgctcaagac
     1801 attgtcgttt ccggcgcgcc gttcggccca gagcccgccc gactatgacc ggcagctcga
     1861 gtactttgca tccgtcgtgg gcgcggcgcg cgacccgctg tcgggtccgc agattcccgt
     1921 tgccttcgtc gccttcagcg ccttcgattc tccgtggaag cgcggcggca attttcaatc
     1981 gtgggatccc tttacgggct ggttcgattc cagcggccaa gagcggcctg tggtgcaaca
     2041 atggatatgg ctcaggaact gactgtcagc cgcgtccgga ggacgaaaaa tccgatcctg
     2101 gtcatggcgc cactgatgct gatggtggcg ttcacggtgt ttctgctggc gccgtggatg
     2161 agccgcgtcg atctgcacga tccggtgttg acggccctgt gcttgagcaa tacggccgtg
     2221 tggtggattg tcctgtggtg gtcgctgcat catttgacgt tcaaggtggg gtcgttgttt
     2281 cggtcggacc gagggcatgc attgccgcag gctgccgcca gcgggctgcg gatgacggtg
     2341 ctgtatccca cgtgcaacga ctttcagtgg caagcttgcc attcgtgcgt gtcgcaggac
     2401 tattcggccg ctcagtatca ggtcgtcatt tgcgacgaca gcagcagcga aagctatcgg
     2461 aagcgaatcg acgagtttca gcgaaccttc ccggaggtga tcgtgctgcg ccgcaatcgg
     2521 cgtcagggat tcaaggcggg caacctcaat cacgccctcg cctcggcgca ggtcaccggc
     2581 gagtggctga ttatcgtcga tgccgatcaa atccttccgc agcactatgt ttcgcagttt
     2641 gccggatttg tcgctggatt gccggatcgc gtcggctacg cgcaggcgcg gcaggaatcg
     2701 gaccacgtgt tgcccacgag ctcgtcgcac tttcagcagg ccttgggacg cgaaatcgat
     2761 atcttctatt cgcacgactt ggcgctgcgg tcggagttcg gctttatgcc gctggtcggt
     2821 cacggcgcgg ctctccgccg aacggcgtgg caacaagtgc cgtttcccga ggtggtctcc
     2881 gaagacttcg cgatgagtct ggagatgcgc aatcgcgatc tggtcggagc gtacgcaccg
     2941 gaggttcagg cctgggaatc gttcccgccc tcgtttggcg cattcttgat ccgattgcgg
     3001 aagtttgcgg gcggaacggc ggagctatac cgcaagcact tgcccgcatt tcttttcggg
     3061 ccggcgacat tcaccgaaaa gctggacctg ttgatgatga tcggttggta cgccctgatg
     3121 c
//
LOCUS       DASUGB010000487         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1305537, whole genome shotgun sequence.
ACCESSION   DASUGB010000487 DASUGB010000000
VERSION     DASUGB010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305537"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..705
                     /gene="hslV"
                     /locus_tag="VFV87_09365"
     CDS             163..705
                     /gene="hslV"
                     /locus_tag="VFV87_09365"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5104006.1"
                     /translation="MRFRSTTILTVRHQGAVALGGDGQVTLGNSIMKADATKIRRLTG
                     GNVLCGFAGSSADAFALLERFEAKLKDNPGNVPRAATELAKEWRMDRALRRLEALMVV
                     ADAKHSLLISGTGDVIQPTDGIIGIGSGGSLAVAAAKALVAHSSLPATQIVREALSIA
                     GQIDIYTNQNIVVEELPCQA"
     gene            693..2057
                     /gene="hslU"
                     /locus_tag="VFV87_09370"
     CDS             693..2057
                     /gene="hslU"
                     /locus_tag="VFV87_09370"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5104007.1"
                     /translation="MPGLMESTADIVARVGELTPRQIVVELDRYIVGQSDAKRAVAIA
                     IRNRWRRQQLPEELRQEVAPKNILMIGPTGVGKTEIARRLAKLTGAPFIKVEATKYTE
                     VGYYGRDVESIVRELVDNSIALVRQRERVKIEEEARRRSTEKILDMLISAARRSRDVV
                     DLEPLETDRDKLRDQLLAGELEDRTVEITVETRATPIMVGGVGMEHLDIDLQGMFDKI
                     MPKQSSRRELSVTQARQIIFEQQCDELLDPDKINSAAIDLAENLGIIFIDELDKIVAS
                     DSRGADVSRQGVQRDLLPIVEGTQVQTRYGYVGTEHILFVAAGAFHKAKPSELMPELQ
                     GRFPIRVELQDLTKDDFVRILKEPKNSLTRQYAALLETEGITLEFTDEAIETLAEFAY
                     SVNQTTQNIGARRLYTILERLVEELSFEAPDMKMGSVQVTAQYVQDRLKQVAEDEDLS
                     RYIL"
     gene            complement(2355..3146)
                     /locus_tag="VFV87_09375"
     CDS             complement(2355..3146)
                     /locus_tag="VFV87_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104008.1"
                     /translation="MNRGIRLAWLSVSILTLALQAAQANEPEESIGLFEAIRAGHLNA
                     QVIPKNSQGGTVVFTNQTGRPLSIRLPAAFAAVPVVAQFGVGGAGGGLGIGGNNGVNG
                     GASANQGLGGGFGGAGGFGGGLNGGGLGGGFFNIGPERVIKLKFVAVCLEHGKRDPTP
                     RIPYELEPIETCTKQSSVVELVKMLSSGEADQSSAQAAAWHLANGMKFEELKRKVGAR
                     HLSGETAPHFTVSQIRQAQSLSAEARRRAKNAAAAASPVSTRSGG"
     gene            complement(3314..>4766)
                     /locus_tag="VFV87_09380"
     CDS             complement(3314..>4766)
                     /locus_tag="VFV87_09380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5104009.1"
                     /translation="DDDLILLGLLPDLDDAHSILTISVRDPGGNVAEGFRPKVRRTEL
                     PLTKSATEAVAGNAGVDLAGYGDYRGVPVVGAWEWLPKYELGLITEIDYSEAYRPLTI
                     LRWAFFAMFALLALSSMAIFVFTVVVARLQREAQKAAIEAKHLGQYRLEVRLGAGAMG
                     VVYKGHHAMLRRPTAIKMLNVDKVNDASIERFEREVQITCKLNNPNTVAIYDYGRTPE
                     GVFYYAMEYLDGIDLQALVEKYGPQPEGRVISVLKQICSSLYEAHSLGLVHRDIKPSN
                     VMLNRRGGEPDVVKVLDFGLVRALDEGKRAAASGGLTGTPLYMSPEAIQTPDLVDARS
                     DLYAVGAIGYFLVTGQPVFNATSLVDLCQQHVSGLPVVPSERLGKPVSHELEAALLAC
                     LEKTRAKRPQTARDLSSLLDRAPTANAWTLDDAEAWWGRHERGAASASSARMPGSSSA
                     TTTGTPPASSASNATGNRTSSGGFDQTLASGEH"
BASE COUNT          913 a         1574 c         1457 g          822 t
ORIGIN      
        1 agaagactac gcaacatggc tttctccagc gacaggtggg agatgtggag ggcggcggaa
       61 cgggtcaggc gcactcgccc ggaccttacc gtatgattgt accgaatgcc tcattgcaag
      121 gctaccaaaa cgcgactcag attcgagctc gaacacctgc cgatgcgctt tcgctccacc
      181 accatcctca ccgtccgcca ccagggcgcc gtcgctttgg gcggcgatgg ccaggtcacg
      241 ctcggcaatt cgatcatgaa ggccgacgcg accaaaatcc gccggctgac ggggggcaat
      301 gtcctgtgcg ggtttgcggg gagcagtgcc gacgccttcg ccctgctgga gcggttcgag
      361 gcgaagctga aggacaaccc cggcaacgtg ccgcgagccg cgaccgagct ggcaaaggaa
      421 tggcgcatgg accgggcctt gcggcggctg gaggccctga tggtcgtggc cgacgccaaa
      481 cacagcctgc tcatcagcgg caccggcgac gtcatccagc cgacggacgg catcatcggc
      541 atcggctcag gcggcagcct ggcggtggcg gcggccaagg ccctcgtcgc ccattccagc
      601 ctgccggcca cgcagatcgt ccgcgaggcc ctctcgatcg ccggccagat cgatatctac
      661 accaatcaaa acatcgtcgt ggaggagctt ccgtgccagg cttgatggaa tcgacagccg
      721 acatcgtcgc ccgcgtgggc gaattgactc cgcggcagat cgtggtcgag ctcgaccgct
      781 atatcgtcgg ccagagcgac gccaaacggg ccgtggcgat cgccatccgc aaccgctggc
      841 gacggcagca gctcccggaa gagctgcggc aggaagtcgc gcccaagaac atcctgatga
      901 tcggccccac cggcgtcggc aagaccgaaa tcgcccgccg cctggccaag ctcaccggcg
      961 ccccgttcat caaggtcgaa gcgaccaagt ataccgaggt cggctactac ggccgcgacg
     1021 tcgagagcat cgtccgcgag ctggtcgata actccatcgc cctggtgcgg cagcgcgagc
     1081 gggtcaagat cgaggaagaa gcccgccgcc gctccacgga aaagattctc gacatgctca
     1141 tctccgcggc ccgcagaagt cgcgacgtgg tcgatctgga gccgctggaa acggatcgcg
     1201 acaagctccg cgatcaactt ctggccggcg agctcgaaga ccgcaccgtc gagatcaccg
     1261 tcgaaacgcg ggccacgccg atcatggtcg gcggcgtcgg catggagcac ctcgatatcg
     1321 acctgcaagg catgttcgac aagatcatgc ccaagcaatc cagccgccgc gagctgagcg
     1381 tgacgcaggc ccggcagatc atcttcgagc agcaatgcga cgagctcctc gacccggaca
     1441 agatcaactc cgccgcgatc gacctggccg aaaatctggg catcatcttc atcgacgagc
     1501 tggataagat cgtcgccagc gacagccgcg gggccgatgt ctcgcggcag ggcgtgcagc
     1561 gcgatctgct ccccatcgtc gaaggaacgc aggtgcagac ccgttacggc tacgtgggga
     1621 ccgagcacat cctgttcgtc gccgccggag cgtttcacaa ggccaagccc agcgagctga
     1681 tgcccgaatt gcagggccgc tttccgatcc gcgtcgagct gcaggacctg accaaggacg
     1741 atttcgtccg catcctcaaa gagccgaaga actcgctcac ccggcagtac gcggccctgc
     1801 tcgaaaccga aggcatcacg ctcgaattca cggacgaggc aatcgaaacg ttagccgagt
     1861 tcgcctatag cgtgaaccag accacgcaga acatcggcgc ccgccggctt tatacgatcc
     1921 tggagcggct ggtcgaggaa ctctcgttcg aagcccccga catgaaaatg ggcagcgtcc
     1981 aggtcaccgc ccagtacgtc caagaccgcc tcaaacaggt cgccgaggac gaagacctga
     2041 gtcgatacat tctgtaggca caaatggcat ctggcgtcct ttcactacct acaacgatca
     2101 acttgcatcg cccactgcag ggctcgtcgc cgcagaggcc tgattgttcg gctgcttcgt
     2161 ttggaactgg caagtgagtt gctccgccgg ggtaagcctg gaggtcgctg ttccgacctt
     2221 ttttgtgccg cctccgccgg tgcagttgaa atgagaataa atcagggcag gacgccagtc
     2281 tggccaattt gcatcgcagc gggatatccg ccggacactt tctctcggcg gatcgtcacc
     2341 tctaccgacc agattcaccc accgcttcga gtggatacgg ggctggccgc agcggctgca
     2401 ttcttcgccc gccgcctggc ttcggcactg agcgactgcg cctgccggat ctggctgaca
     2461 gtgaaatgtg gggcagtctc gccgctaaga tgccgcgccc caacctttct cttcagttcc
     2521 tcaaacttca tgccgttggc caaatgccat gctgcagctt gcgcgctgga ctggtcggcc
     2581 tcgccgctgc taagcatttt gacgagctcc acgaccgacg attgttttgt gcacgtctcg
     2641 atcggctcca gctcgtaagg gatgcgcgga gtcggatcgc gtttgccgtg ctccaggcag
     2701 acggcgacaa atttgagctt gatgacccgt tcggggccga tgttgaaaaa ccctcctcca
     2761 agtccgccgc cgttgagccc gcctccgaaa cctcccgcgc cgccgaatcc gccgcccagt
     2821 ccctgattgg cgctcgcgcc gccgttgact ccattgttgc cgccaatccc tagcccgccc
     2881 cccgctccgc caacgccaaa ctgagcaacg acgggcacgg ccgcgaatgc tgcgggcaag
     2941 cgaatcgaaa gcggccgacc ggtctggttg gtaaaaacga ccgtcccgcc ctgcgagttc
     3001 ttcggaatga cctgcgcatt gagatgacca gctcttatcg cctcgaacaa gccgatcgac
     3061 tcctccggct cgttagcttg agccgcctga agtgccaatg tcagaatgga aacgctaagc
     3121 catgccaaac ggattccgcg gttcatcgaa acctctccct accccttggc ccaatccccg
     3181 gttgctggcc cgatggaagg gcgcccttgc gggcttcgat gtcgccaaat gcgcggtggt
     3241 tacgggaatt gccaaagact gggcagcgga cgttgtgaag ccggcggtca ttgaattgcc
     3301 gcggctgtga gcttcagtgt tcgccgctcg ccagagtttg atcaaatcct ccgctggagg
     3361 tgcgattgcc cgtcgcgttc gaagcgctgg aggcaggcgg cgtgccggtg gttgtcgcgg
     3421 atgagctacc tggcattcgg gcgctcgacg cagacgccgc gccccgctcg tgccggcccc
     3481 accacgcttc ggcgtcgtcg agcgtccagg cattcgcggt cggcgcgcgg tccagcaagg
     3541 aggatagatc gcgagcggtc tgcggacgct tggcccgcgt cttctccagg caggcaagca
     3601 gcgccgcttc caattcgtgc gacaccggct tgccgaggcg ttccgaagga acgaccggca
     3661 ggcccgatac gtgctgctgg cacagatcga ccagcgatgt ggcgttaaac accggctggc
     3721 cggtgaccag gaagtagccg atcgcgccga cggcatacaa gtcgctgcgg gcatcgacca
     3781 ggtcgggagt ctggatcgct tcgggcgaca tatagagcgg cgtccccgtc aatccgccgg
     3841 atgccgccgc ccgcttgcct tcgtccagcg cccggacgag accgaaatcg agcaccttca
     3901 ccacgtctgg ctcgccgccg cggcggttga gcatcacgtt ggacggcttg atgtcgcgat
     3961 gcaccagccc cagcgaatgc gcctcataga gcgagctgca aatctgcttg aggacgctga
     4021 tcacgcgccc ttcgggctgc gggccgtact tctcgacgag ggcctgcaag tcgatcccgt
     4081 caagatactc catcgcgtaa taaaagaccc cttcgggcgt gcggccgtag tcgtaaatcg
     4141 cgacggtgtt cggattgttc agcttgcacg tgatctgcac ttcgcgctcg aaccgctcga
     4201 tcgaagcgtc gttgacctta tcgacgttga gcattttgat cgccgtcggc cggcggagca
     4261 tggcgtggtg ccccttatag acgacgccca tcgcccccgc cccgagccgc acttcgaggc
     4321 gatactgccc aaggtgcttg gcctcgatgg ccgccttctg agcttcgcgc tgcaggcggg
     4381 cgacgacgac ggtgaagacg aagatcgcca tcgagctgag cgcgagcagg gcgaacatcg
     4441 cgaagaacgc ccaccgcagg atcgtcagcg ggcgataggc ctcggagtaa tcaatctccg
     4501 tgatcagccc cagctcgtac ttcggcagcc attcccacgc gccgacgacg ggcacgccgc
     4561 gatagtcgcc gtagccggcc agatcgacac ctgcattgcc ggcgactgct tcggtcgcgg
     4621 atttcgtgag cggcagttcg gtccgccgca ccttcggccg aaagccctcc gccacgttgc
     4681 caccggggtc gcggaccgag atcgtgagaa tcgagtgcgc atcgtcgagg tccggcaaga
     4741 gtcccagcag aatcaggtcg tcatcg
//
LOCUS       DASUGB010000488         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_195301, whole genome shotgun sequence.
ACCESSION   DASUGB010000488 DASUGB010000000
VERSION     DASUGB010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195301"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..611)
                     /locus_tag="VFV87_09385"
     CDS             complement(342..611)
                     /locus_tag="VFV87_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104010.1"
                     /translation="MLKFLAAGVVGCAGLVCTADQAAAGHRGGCCAPCAPAAVAPAPA
                     PMAQAPQGYRAYSYQPAPSSYRAYGTWVTGRAYENAANKSLGRGF"
     gene            complement(817..1326)
                     /locus_tag="VFV87_09390"
     CDS             complement(817..1326)
                     /locus_tag="VFV87_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104011.1"
                     /translation="MFGRFRKLAARSRQRLLSLVMLPAFFLATLPHTACICGDGHRVA
                     RCNAVACCAIKAGKSTGVSCGCPCCQSHSGKPCCCKAKQAKPACDKCPLPGLAAKTGN
                     CCQPVIEAPAPAKSVEKVSPVSPLDVAIGGLPPTFADAAPGLLARRVLDHHGPPPVDV
                     VIVYLHLTI"
     gene            1839..2129
                     /locus_tag="VFV87_09395"
     CDS             1839..2129
                     /locus_tag="VFV87_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104012.1"
                     /translation="MRAPWLWTNFTIIALGLWLATSPFTFGYLRSDLSGLVNQVTAER
                     GLASAETRGMVMACNDSLSGIVLALLAALALWPHPRTDFWGRWGVCAVGVWL"
     gene            2142..3224
                     /locus_tag="VFV87_09400"
     CDS             2142..3224
                     /locus_tag="VFV87_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5104013.1"
                     /translation="MLWSPSPAAYVIDTLIGAFAIGLSVLVPMMPGMAHHMVMLKPGP
                     EVPPGWTYNPSSWHQRAPLIGLAIVGWFISRYLAAVQLGYIDRAWEPLFGEGTQLVLH
                     SDVSRAWPISNAGFGAAAYTFEALMGFMGTKSRWRTMPWMVTFFGILVVPLGATHLIL
                     VVLQPVLVGHWCTLCLAAAIVMLVMVPLAVDEVVAMCQFMARAVREGRPFWRTFWVGG
                     TLPKQGEDRRTPRFGSPLAPMITATVWGVSLPWPLVVTMLLGLWLIVAPDFLGTMKPA
                     ADSSRLAGALVITIAAVATAEVTRAARFFNVLLALWVAGAPWLVAEESALGTVNALIV
                     GLLLIPMCIPRGIVRERYGGWDRYVV"
     gene            3306..3506
                     /locus_tag="VFV87_09405"
     CDS             3306..3506
                     /locus_tag="VFV87_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104014.1"
                     /translation="MNQATNILLIVLAVIGAIALFGAACMWLMYGMMMGPGMASGFGL
                     LLALVIIAIAVVLLLTRTKAAS"
     gene            3750..>4521
                     /locus_tag="VFV87_09410"
     CDS             3750..>4521
                     /locus_tag="VFV87_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5104015.1"
                     /translation="MISLRAGSLVALLTLAALGCKTTASSRAAAIAAANTPVPGAAPV
                     QTVRNDAPYANRPAPLPSIAPVAHQEGLPAPSSPLSPEELSPEEMPLDGVVSREWLVA
                     EIEARNPNLAAMLAAWQAAAQRYPQAVALDDPMFMGLIAPDSIGSDEVSTGYMLDLQQ
                     KLPWFGKRRLRGAEAAAEADAAFQDTGDTRLQVRLAAEFAFLEYVLAARLIQLNGENT
                     GIMEQFRETAQVKYRNNLVTQQDVLQADVELAELARRQF"
BASE COUNT          801 a         1386 c         1508 g          826 t
ORIGIN      
        1 aggcgggcag tggagaccgg taatcaggta ggtggctttc gttgtcgttt tcgcgggttc
       61 ttgcgcgccg gcgatcgaag gagcaagaag cgtcaggccg gcaagaccga ccaacaggga
      121 acaaatcatt cgcatggacg actctccaat ggcagggaaa ggagggacaa atgaacaaat
      181 tgcggtgtta tcgaactcct cgcccgccgc cgcaggcgat tggcccacgg cgacggagtg
      241 aagatgtcat ggcataggac aggttttcaa cctgtcctgg tgcggacggt ctggtgcgga
      301 cgggttgaaa acccgtccta cttccggtcc gtcgtcttca cttagaaacc gcggcccagc
      361 gacttattgg cggcgttctc ataagcccgg cccgtcaccc acgtgccata ggcgcggtag
      421 ctcgacggag ccggctggta ggaataggcc cggtagccct gcggggcttg ggccatcggc
      481 gccggtgctg gagcaacggc cgccggagcg catggcgcgc agcaaccgcc gcggtggccg
      541 gcggcagcct gatcggcggt gcaaaccaga cccgcgcaac ccacaacacc agcagcaagg
      601 aacttcaaca tgacaaacac ccctgcaaag gcggcgcgac ggtcattccc tacccaggac
      661 tgcaaccgca cgccggggaa ttcggaacct cgatgcgcac cacagcgcat cacgcatcag
      721 gaaatgcagc ctgcggcaag caacatggct cgcgggggcc gcgggacgat agcgcaacaa
      781 gcagaagggc ggtccacgag cgaccgcgcg ggtgcgctag atcgtcaggt gcagatagac
      841 gatcactacg tccaccggtg gcggaccatg atgatcaaga acgcgccgcg caagcagccc
      901 gggcgccgca tcagcgaaag tcggcggcag accgccgatc gcaacatcca gcggcgaaac
      961 gggcgaaacc ttctccacgc tcttggcggg agcaggcgct tcgatcaccg gctggcagca
     1021 gttgccggtc ttagcggcca atccgggcag cggacacttg tcacaagctg gcttggcctg
     1081 cttcgctttg cagcagcaag gcttgccgct gtgggattgg cagcacgggc agccgcacga
     1141 gacaccggtc gatttgccgg ctttgatcgc acaacaagcg accgcgttgc accgggctac
     1201 tcgatggcca tcgccgcaga tacacgcggt gtgcggcagc gtcgccagaa agaacgccgg
     1261 cagcatgacc agcgagagca atcgctggcg gctgcgcgct gcaagttttc tgaaacggcc
     1321 aaacatagga aactcgtgat gacccaatct tagcggccac tcgcctcgat tccagtgcgg
     1381 tggacctgca cagcatactc acgatcgtcc gcaatcggca aagccaattt gcaaagtttc
     1441 gccagcagaa atacgcacaa ctgcgccggc cggttcgccc gcaggtcagc cagagggcgt
     1501 ttgcctccct cataagtata gctcaggctg gagatttgac ccaatgtcgg cgcagcattt
     1561 gccgcgattt ttcgaagccg actgacttca tgtaggagag cgggaccgct ggccggcgtg
     1621 acgagcgaga gttgttgacc atggcgacgg cacaccaaca ggctgctctg ttcacctgtc
     1681 cgatgcatcc ggaagtcgag caggaccagc cgggttcttg tccaaagtgc ggcatggcgc
     1741 tgctcccgag cgaagccgat ggggctgagc agcaggaagg cgagcaccag aatcacgagg
     1801 cgatggcggc tggcgaccat caagagatgg tccggcagat gcgcgctcct tggttgtgga
     1861 ctaactttac gatcatcgcg ctgggactgt ggcttgcgac cagccctttc acgttcggct
     1921 atctgcgctc cgacctcagc gggctggtca atcaagtgac cgcggaacgc ggcctcgcct
     1981 cggccgaaac ccgtggaatg gtgatggcct gcaatgactc tctgagcggc atcgtactgg
     2041 ctctcctcgc ggctctggcg ctttggccgc atccccgcac cgacttctgg ggacggtggg
     2101 gcgtttgtgc ggtgggggtc tggctttaat ttgcaccgct ggtgctgtgg tctcccagcc
     2161 cggctgccta cgtcatcgac acgctgatcg gggcgtttgc gatcggtctc tcggtattgg
     2221 tcccgatgat gcccggcatg gctcatcaca tggtgatgtt gaagcccggc ccggaggttc
     2281 cgcccggctg gacgtacaac ccttcctcct ggcaccagcg ggcgccgctg atcgggctgg
     2341 cgatcgtcgg ctggttcatc tcgcgttacc tggccgcggt gcagcttggt tacatcgacc
     2401 gcgcctggga gccgctgttc ggggagggca cccagctcgt gctgcattcg gacgtatcgc
     2461 gggcgtggcc catctccaat gcggggtttg gcgcggcggc ctataccttt gaagcgctca
     2521 tgggcttcat gggaaccaag agccggtggc gcacgatgcc ttggatggtg acgttttttg
     2581 gcattctcgt cgtgccgctc ggcgcaacgc acctgatcct tgtcgtcctg cagccggtgc
     2641 tggtcggcca ttggtgtacc ctctgtttgg ctgctgcgat cgtcatgctc gtgatggtgc
     2701 cgctggccgt ggacgaagtg gtcgccatgt gccaattcat ggcgcgcgcg gttcgcgagg
     2761 gccggccgtt ctggcggacg ttttgggtag gaggcacgtt gccaaaacag ggcgaggatc
     2821 gcagaacgcc gcgattcggc tcgcccctgg cgccgatgat cacggcgacg gtatgggggg
     2881 tgagcctgcc gtggccgctc gttgtcacga tgctgctggg cctttggctc atcgtcgccc
     2941 ccgatttttt ggggactatg aagccggccg ccgacagcag ccggctggcg ggagcgctcg
     3001 tcatcacgat tgccgccgtc gcaacggccg aagtgacccg cgcggcgaga ttcttcaacg
     3061 tcctgctcgc cctgtgggtt gccggggcgc cgtggctagt agcggaagag agtgcacttg
     3121 ggaccgtcaa cgctttgatc gtggggctgt tgctgatccc gatgtgcatc ccgcgcggca
     3181 tcgtgagaga gcgctacggc gggtgggacc ggtacgtggt gtgagacaag gaacgtggcc
     3241 gaaagggaga cggagactgg caaccgaggg tgggatcgaa cgaagaaagc gtgggagaca
     3301 aaacgatgaa ccaggcaacg aacattctgt tgattgtgct ggccgtgatt ggcgctatcg
     3361 cgctgtttgg ggcggcatgc atgtggctga tgtacggcat gatgatgggt ccgggcatgg
     3421 caagcggatt cgggcttctc ctggctctgg tgatcatcgc catcgcggtc gtcttgctcc
     3481 tcacgaggac aaaggctgcc tcgtaggcat tgatccccgc tggcagcaac gacgacgcta
     3541 gcggttgcct ggaaagatct atcgcgcgga acaatttttc tgttcgcgat ttgccgagag
     3601 cgccgatttc aacgacacgt tcaccagccc acccgttggc gctgccggcc ttgtgcgccc
     3661 cgcatcgggg tcccacctgt tcgtgcaagt cctcccgcat tcaaacgcga ggcttgcgcc
     3721 ccattcctgt ttcacccggg gaggcgagca tgatttcgct ccgcgccggg agcctggtcg
     3781 cgctgctaac gctcgcggcc ctgggctgca agacgacggc cagctcgcgc gctgctgcga
     3841 tcgccgcggc aaatacgccg gtgccgggcg ctgcgccggt gcagacggtt cgcaacgatg
     3901 ctccttacgc gaatcgcccc gcgccactcc cgtcgatcgc cccggtcgcg catcaggagg
     3961 gtctgcccgc gccgtcgtct cccttgtcgc ccgaggagtt gtcgcccgaa gagatgccgc
     4021 tcgacggagt tgtgtcgcgc gagtggctgg tggcggagat tgaggcccgc aatcccaatc
     4081 tggcggcgat gctcgcggcg tggcaggcgg cggcccagcg ctatccccag gccgtggccc
     4141 tcgacgaccc gatgttcatg ggactcatcg cgccggactc gattggctcc gacgaggtga
     4201 gcacggggta catgctcgat ttgcagcaga agctcccgtg gttcggcaag cggcgcctgc
     4261 gcggcgctga ggcggcggcg gaagcggatg ccgccttcca ggacacgggc gacacgcgtc
     4321 tgcaggtccg cctggccgcc gagttcgcct ttctggagta cgtgctggcc gcccggctga
     4381 tccagctcaa tggcgagaat accgggatca tggagcagtt ccgcgagacg gcccaggtca
     4441 aataccgcaa caatctggtg acgcagcagg acgtgctgca ggccgacgtc gagctggccg
     4501 agctggcccg ccggcagttc g
//
LOCUS       DASUGB010000489         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1585085, whole genome shotgun sequence.
ACCESSION   DASUGB010000489 DASUGB010000000
VERSION     DASUGB010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585085"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2000
                     /locus_tag="VFV87_09415"
     CDS             <1..>2000
                     /locus_tag="VFV87_09415"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5104016.1"
                     /translation="DKSKKGPEFRARDVMQRMAECAWQCGDPGVQYDTTINRWNTCPN
                     SGRINASNPCSEYMFLDNTACNLASINLMKFRRAGGNAAGNSADGTFDVERFKAACRI
                     FFIAQEILVDHASYPTADIARNSHLFRPLGLGYSNLGSLLMSEGLPYDSEAGYGVCGS
                     VTALLHGAANLASAEMAAVVGPFEGFHKNREPMLRVMQMHRDAVEEINDAGPQYLKDA
                     ARALWDEVVALGKKVGYRNAQATVLAPTGTISFMMDCDTTGIEPDIALVKYKQLAGGG
                     MLKIVNQTVPLALAKLGYDDPQIKSIVKYVNEQDTIEGAPDLAAEHLPVFDCAFKPAN
                     GIRSIQWRAHVRMMAAAQPFLSGAISKTVNMPADVTPDDIAEAYFWGWELGLKALAIY
                     RDGSKQSQPLNTKSEEGKKDEKVVVVSKPRRERLPDTRNSITHKFNVGGHEGYINVGM
                     YDPVVPGEQGRPGELFITMAKEGSTVGGLMDAFGTAISMSLQYGVPLADLVNKFSHMR
                     FEPMGHTTNPDIRIAKSVIDYIFRWMGIRFLEGYKEANSGLPAGGGKSSLVPEDDDAS
                     ARLPAATKAGGQSAGQGTATPARSASEGVAVQDSGFRVQDSPATGAKATPPANGHANG
                     SSKRAEINPTLRIDGNGGPSAGSVSDAPSLRSEQFARFQLDAP"
BASE COUNT          409 a          644 c          646 g          301 t
ORIGIN      
        1 gacaagtcga agaaaggtcc cgagttccgt gcccgcgacg tgatgcagcg gatggccgag
       61 tgcgcctggc agtgcggcga cccaggcgtg caatacgaca cgacgatcaa ccgctggaac
      121 acctgcccca actcgggccg catcaacgcc agcaatccgt gcagcgagta catgttcctc
      181 gacaacacgg cttgcaacct ggccagcatc aacctgatga agttccggcg cgccggagga
      241 aacgctgctg gaaacagcgc cgacggcacg ttcgacgtcg agcggttcaa agccgcctgc
      301 cgcatctttt tcatcgccca ggaaatcctg gtcgatcacg ccagctatcc gacggccgac
      361 atcgcccgca acagccacct gttccgccca ctaggcctgg gctattcgaa cctgggcagc
      421 ctgctgatga gcgaggggct gccctacgac agcgaggcgg gctatggcgt ctgcggatcg
      481 gtcaccgctc ttttgcacgg ggcggccaac ctggccagcg ccgagatggc ggcggtcgtc
      541 ggcccgtttg aaggcttcca caagaatcgc gagccgatgc tccgtgtgat gcagatgcac
      601 cgcgacgcgg tcgaggagat caacgacgcc gggccacagt acctgaagga cgcggcccgc
      661 gccttgtggg acgaagtggt ggctctcggc aagaaggtcg gctatcgcaa tgcccaggcg
      721 accgtgctgg ccccgaccgg cacgatcagc ttcatgatgg actgcgacac gaccggcatc
      781 gagccggata tcgccctggt gaagtacaag cagctggccg gcggcggcat gctgaaaatc
      841 gtcaaccaga ccgtgccgct ggccctggcc aagctcggct acgacgatcc gcagatcaag
      901 agcatcgtca aatatgtcaa cgagcaggac acgatcgaag gagcgccgga cctggcggcc
      961 gagcacttgc ccgtgttcga ttgcgccttt aagccggcca atggcattcg cagcatccag
     1021 tggcgggccc acgtgcggat gatggcggcc gctcagccgt ttttatcggg tgcgatcagc
     1081 aagacggtga acatgccggc cgacgtgacg cccgacgaca tcgccgaggc gtacttctgg
     1141 ggctgggagc tgggcctgaa ggccctggcc atctatcgcg acgggagcaa gcagagccag
     1201 ccgctcaata ccaagtcgga ggagggcaaa aaggacgaga aggtcgtcgt cgtcagcaag
     1261 ccgcggcgcg agcgattgcc cgatacgcgc aactcgatca cccacaagtt caacgtgggg
     1321 gggcacgaag ggtatatcaa cgtcggcatg tacgaccccg ttgttcccgg agaacagggg
     1381 cggcccggcg agctgttcat cacgatggcc aaagaaggct cgaccgtcgg cggcctgatg
     1441 gacgctttcg gcacggcgat cagcatgtcg ctgcagtatg gcgtgccgct ggcggacctg
     1501 gtgaacaagt tctcgcacat gcggttcgag ccgatgggtc acacgaccaa tcccgacatc
     1561 cgcatcgcca agagcgtgat cgactacatc ttccgctgga tggggatccg cttcctcgaa
     1621 gggtacaagg aggccaacag cggcctgccc gccggcggtg gcaaatcatc actcgtaccg
     1681 gaggacgacg acgcctcagc tcgcttgccc gccgccacga aggccggcgg gcagtcggcc
     1741 ggtcaaggaa cggccacacc agcccgcagc gcaagcgagg gggtagcggt tcaggattca
     1801 gggttcaggg ttcaggattc acccgccacg ggcgccaagg cgacgccgcc tgccaacgga
     1861 cacgccaacg gttcttccaa gcgggcggag atcaatccca ccctgcgaat cgacggcaac
     1921 ggcggcccgt cagccggcag cgtcagcgat gctccgagcc tgcggagcga gcagttcgcc
     1981 cgtttccagc tcgacgctcc
//
LOCUS       DASUGB010000490         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_750426, whole genome shotgun sequence.
ACCESSION   DASUGB010000490 DASUGB010000000
VERSION     DASUGB010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750426"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFV87_09420"
     CDS             complement(<1..1334)
                     /locus_tag="VFV87_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEX5104017.1"
                     /translation="MSEPAKPSPTEGIKEQSRFLRGDLAPELHDGLDHFGKTSESLLK
                     FHGTYQQDDRDERGAGGVDEEGKKKKSYIFMVRTKIPGGKLTSRQLLDELDLCDQVGN
                     TTLRITSRQGLQLHGVIKRNLKEAIARINHTQLSTLGACGDVNRNVMCCPAPIGDNLH
                     RQTQALADHLAAYFAPRTKAYYETWLTDSASGEKQLVGGGPDADVNGAGQGDGFDVEP
                     IYGTHYMPRKFKMAIGFTFDNCVDVYANDLGLIAVTRGDEIVGYNLIAGGGMGVTPSA
                     KKTFPAVAQQIAYVTPDQVHRAVEAVFKVQRDYGNREDRKLARLKYVIANWGVAAFKG
                     KVEEYFGGPLAEFQRENDVRGFDDHMGWAEQRDGRWFYGLNIENGRIKDTPEMQLKTA
                     LRQVCRELDPSIRTTPHQSLLLGNIQPQDRSRLEAIFRSHGVRLTEEISTVRR"
     gene            complement(1529..2440)
                     /locus_tag="VFV87_09425"
     CDS             complement(1529..2440)
                     /locus_tag="VFV87_09425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5104018.1"
                     /translation="MGFQDRDYYRGQTAAGPLGGSVVVKLIAINIAVFLANLFLGQSS
                     DPRHDEWIMNALALKPDTLLHPLQYYRFLTYGFAHAPRELWHIGLNMVGLFCFGTALE
                     QRYGWKEFLRFYLIAILLGGLAWALRMYLLDHSLVTNFGGRPFPVDGPSLLGASGGIT
                     AAVILFCLLYPRATLLMMFVIPMPAWIAGLVIVLLDMFGGREGVAHDVHLTGAAFAVA
                     YWGLGWNFGRLPGMSGARRAIRALAKRMQPRPDLRVHDPEAYYEDLDAEADKLLEKVA
                     REGEGSLSDRERRVLEAYSRRMRQKLS"
     gene            complement(2560..>3091)
                     /locus_tag="VFV87_09430"
     CDS             complement(2560..>3091)
                     /locus_tag="VFV87_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104019.1"
                     /translation="GRAQLDRLRRAKPGQKAADMEFASGETATFDRATADKLIADGVA
                     EKVQPIYRRRLTDFELKFHGLHSQIVEINSRIRALSQDVAAIQTSKAKADAQATLLED
                     YRAKLTADRDKVKFERDELKKYSEAVAGRLSEVRSELSQLYRSNKALSRELAALNAQM
                     TEEIEQRTRAATARAN"
BASE COUNT          544 a          971 c          978 g          598 t
ORIGIN      
        1 cggcgaacgg tcgaaatctc ctcggtcaac ctcacgccgt ggctgcggaa aatggcttcc
       61 agccgcgagc ggtcctgcgg ctggatattg cccaacagca ggctttggtg cggggtggtg
      121 cggatcgagg gatccagctc gcggcagacc tggcggagcg ccgttttcag ctgcatctcg
      181 ggtgtgtcct tgatgcggcc gttttcgata ttgaggccat agaaccagcg gccgtcccgc
      241 tgctcggccc agcccatatg gtcgtcgaag ccgcggacgt cgttttcccg ctggaattcg
      301 gccagcggcc cgccgaaata ctcctcgacc ttccccttga acgcggccac gccccaattc
      361 gcgatcacgt acttcaggcg ggcgagtttg cggtcctcgc ggttaccgta gtcgcgctga
      421 accttgaaaa ccgcttcaac ggcccgatgc acctggtccg gcgtcacata ggcgatttgc
      481 tgcgccacgg cgggaaacgt cttcttggct gagggggtga cccccatgcc gcctccggca
      541 atcaggttgt agccgacgat ctcgtcgccg cgcgtgaccg cgatcaggcc taggtcgttg
      601 gcatacacat cgacgcagtt gtcaaaagtg aagccgatgg ccatcttgaa cttgcggggc
      661 atgtagtgcg tgccatagat cggctcgacg tcgaagccgt ccccctggcc cgccccattc
      721 acgtccgcgt ccgggccgcc cccaacgagt tgcttctcgc cgctggccga gtcggtgagc
      781 caggtttcgt aataggcctt ggtgcgagga gcgaaataag ccgccaggtg atcggccagg
      841 gcctgcgtct ggcggtgcag gttgtcgcca atcggggccg ggcagcacat gacgttgcgg
      901 ttcacgtcgc cgcaagcgcc cagcgtgctg agttgcgtgt ggttgatgcg ggcaatcgct
      961 tccttcagat tccgcttgat cacgccgtgc aattgcagcc cctgccgcga cgtgatccgc
     1021 agcgtggtgt tgcccacctg gtcgcacaga tcgagctcgt cgagcaattg gcggctggtc
     1081 aacttgccgc ccgggatctt cgtgcggacc atgaagatgt aggacttttt tttcttcccc
     1141 tcctcatcga cgccccccgc gccgcgctcg tcccgatcgt cctgctgata ggtgccgtgg
     1201 aatttgagca gcgattcgct ggtcttgccg aaatgatcga ggccgtcgtg cagctcgggc
     1261 gcgagatcac cgcggagaaa gcggctttgc tctttgatgc cttcggtggg actgggcttg
     1321 gcgggttcgg acatgagcat ggccctggcg aagggcgatt ggactgttgg tcagcgtaga
     1381 attcgtgcgt cgaaagcggg cagcgggccg aatgattacc aagtttagca atattatgat
     1441 gattcggccg agccagtcaa gggagtatag ccaatccagg gaaattctcc tacgccgcca
     1501 gtgtcgtgac acaccaaatc gccagctgtc aactgagctt ttgccgcatg cgacggctat
     1561 aagcttccag gacccggcgt tcgcgatcgg aaaggctgcc ctcgccttcc cgggcgactt
     1621 tttccagcag cttgtctgcc tcggcgtcga gatcttcgta atacgcctcg gggtcatgaa
     1681 cgcggaggtc cggccggggt tgcattcgct ttgccagcgc gcgaatggcc ctgcgcgcgc
     1741 cggacattcc cggcaggcga ccgaagttcc agcccaagcc ccaatacgcc acggcaaacg
     1801 cagcgcccgt cagatggacg tcgtgagcca ccccttcgcg gccgccaaac atgtccaaca
     1861 gcacgatcac gaggccggcg atccaggccg gcatgggaat gacgaacatc atcagcagcg
     1921 tcgcccgcgg atagagcagg caaaacagga tgaccgcggc ggtaatgcca ccggacgcgc
     1981 ccaggagcga aggcccgtca accgggaacg gcctgccgcc aaagttcgtg accagactgt
     2041 gatcgaggag gtacatccgc agtgcccacg ccaatccgcc cagcaaaatc gcgatgaggt
     2101 aaaaccgcag gaactccttc cagccataac gctgttccag ggccgtgccg aaacagaaca
     2161 ggccgaccat gttcagcccg atgtgccaca actcacgcgg ggcgtgagca aatccgtagg
     2221 tcaggaaccg gtagtactgg agcgggtgca gcagcgtatc gggcttgagc gccagggcgt
     2281 tcatgatcca ttcgtcatgc ctgggatcgc tcgactggcc cagaaacagg ttggccagga
     2341 acacggcgat gttgatcgcg ataagcttga cgactactga cccgcccagc gggccggcag
     2401 ccgtctggcc gcggtaatag tcccgatcct gaaagcccat ggcgcgaagc gatccggtgc
     2461 gtgcctcgat ccgtccgcaa acacccccta tcttacgcgg cagggcaaag ccgggcgagg
     2521 gcgacgaagc gtggcgggag cggcagcctg cggccggggt tagttcgccc tggcggtcgc
     2581 ggcgcgggtc cgctgctcga tttcttccgt catctgggca ttgagagcgg ccagctcgcg
     2641 gctgagggct ttgttcgagc gatagagttg gctcagctcg ctccgcacct cgctcaggcg
     2701 gccggccaca gcctcgctgt actttttcag ctcgtcccgc tcgaacttca ccttgtcgcg
     2761 gtcggccgtg agcttcgccc ggtagtcttc caacagcgtc gcctgagcgt cggccttggc
     2821 cttcgacgtc tgaatcgcag cgacatcctg cgatagggcg cggatgcggc tgttgatctc
     2881 gacgatttgc gagtgcagcc cgtggaattt gagctcgaag tcagtgagcc ggcggcggta
     2941 gatcggctgc actttttcgg ccacgccgtc ggcaatcagc ttgtcggccg ttgcgcggtc
     3001 gaatgtggcc gtttcaccgg aagcaaattc catgtccgct gccttctggc cgggcttggc
     3061 tcgccgcagg cgatcgagct gcgccctgcc g
//
LOCUS       DASUGB010000491         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_334271, whole genome shotgun sequence.
ACCESSION   DASUGB010000491 DASUGB010000000
VERSION     DASUGB010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334271"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..>2455
                     /gene="fusA"
                     /locus_tag="VFV87_09435"
     CDS             402..>2455
                     /gene="fusA"
                     /locus_tag="VFV87_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5104020.1"
                     /translation="MDLSKLRNFGISAHIDSGKTTLSERILFYSGRIHKMEEVKGDGD
                     GATMDFMDLERERGITITSAATHLEWDGHTMNLIDTPGHVDFTIEVERSLRVLDGAIL
                     VLCAVGGVQSQSMTVDRQMKRYHVPRLAFINKMDRTGARPFSIIDDIKSKLGVDCCLM
                     QYPMGGGEKFEGMIDLVTQKAIKFEGKGGEKVGYVEVPAQYVADVKKYRHHMLETLAM
                     YSDELMELLLSEEEPSEELVHKVTKQAVQEQGFTPVFVGSAYKNKGVQPLLDAIVRYL
                     PSPLEHEVTALKYDNPAEKFKLEPDPAKPFVGMAFKIVEDPFGQLTFVRIYQGTIKKG
                     EMFYNQRTGRKDRFSRIVKMHADKREEIDSAEAGDIVAIMGIDCASGDTYSSEPKYCS
                     LESMFVPKPVIKVAVNPVSRADADKMSKALQRFRKEDPTFNVFNDEETSETIIAGMGE
                     LHLDIYVERIRREYKVELEVGAPKVSYRESPTRKANWNYKHKKQTGGSGQYAHIVGYF
                     EPIEEGTTEETFIFEDEVTGGRIPKNYIPSIEKGFRAVLGKGPLAEYPVVGLKVVVED
                     GSYHEVDSSDRAFQTAAEGCFREHFKDTKPALLEPIMKVEIECPENFQGPITGDLISR
                     RGMIVNTDMRDGITNILAEVPLAETFGYATDIRSMSQGQGTFTMELLCYRKVPASVQE
                     DI"
BASE COUNT          531 a          743 c          758 g          423 t
ORIGIN      
        1 ggacgcgagc cccggttccc attgtaaaac tcccgtgaat tccccctgcc gaaccttaac
       61 ggcacgaact tggcctttcc cgctttcgac tttcggctaa gatgaatggc tgtagcgacc
      121 gtctgccata aggatttagg cagcgccaga ggccttgccg ctgcccgctt agaatggatg
      181 ctctcccgcc ttccagcgtt tcctgactgg ctgggcagac gcgtgcgtgt tgctcgctcc
      241 cggctggtgc tggcgggtgg tgctattgcc actcggcgct gtccggggat gataagaaga
      301 acgttttgcc cggcaggcgt gtcctgccta agtcccggcg cgatcattgc gtgcccgacc
      361 gctcgctttg tcgcccggcg gctcagattt ccaggtgctg tatggacctt tctaagcttc
      421 gcaatttcgg catttccgcc cacatcgaca gcggcaaaac cacgctgagc gagcggattc
      481 tgttctactc cggccgcatc cacaagatgg aagaggtcaa gggagacggc gacggcgcga
      541 ccatggactt catggacctg gagcgcgagc ggggcatcac catcaccagt gccgccacgc
      601 acctggaatg ggacgggcac acgatgaacc tcatcgatac gcccggccac gtcgatttca
      661 cgatcgaggt cgagcgcagc ctgcgcgtgc tggacggggc gattctcgtc ctctgcgccg
      721 tcggcggcgt gcagtcgcag tccatgaccg tcgatcgcca gatgaagcgc tatcacgtgc
      781 cgcggctggc gttcatcaac aaaatggacc gcaccggcgc ccggccgttc tcgatcatcg
      841 acgacatcaa gagcaagctc ggcgtcgatt gctgcctgat gcagtacccg atgggaggcg
      901 gcgagaaatt cgaggggatg atcgacctgg tcacgcagaa ggcgatcaag ttcgagggca
      961 aaggcggcga gaaggtcggc tacgtcgaag tgccggcgca gtacgttgcc gacgtgaaga
     1021 aatatcgcca ccacatgctc gaaacgctgg cgatgtacag cgacgagctg atggagctgc
     1081 tcctctcgga ggaagagccc agcgaagagc tggtccacaa ggtcaccaag caggccgtcc
     1141 aggagcaggg ctttacgccg gtgttcgtgg gctcggctta caaaaacaag ggcgtgcagc
     1201 cgctcctcga tgcgatcgtc cgctacctgc cttcgccgct ggagcatgag gtgacggccc
     1261 tgaaatacga caatccggcc gagaaattca agctggagcc cgatccggcc aagccgttcg
     1321 tcggcatggc gttcaagatc gtggaagatc cattcggtca gctcacgttc gtgcggattt
     1381 atcagggcac gatcaaaaag ggcgagatgt tctacaacca gcgcaccggc cgcaaggacc
     1441 gcttcagccg catcgtcaag atgcatgccg acaagcggga ggagatcgac agcgcggagg
     1501 cgggtgatat agtcgccatc atgggcatcg actgcgccag cggcgatacc tacagcagcg
     1561 aacccaagta ctgctcgctg gagagcatgt tcgtccccaa gccggtcatc aaagtcgccg
     1621 tcaacccggt cagccgggcc gacgcggaca agatgagcaa ggccctgcag cggttccgca
     1681 aggaagaccc gacgttcaac gttttcaacg acgaagaaac gagcgaaacg atcatcgccg
     1741 gcatgggcga gttgcacctc gatatctacg tcgagcgcat ccgccgcgag tacaaggtcg
     1801 agttggaagt cggcgcgccg aaggtcagct accgcgaatc gcctacccgc aaggcgaact
     1861 ggaattacaa gcacaagaag cagaccggcg gctccggaca gtatgcccac atcgttggct
     1921 acttcgagcc gatcgaagaa ggcacgaccg aagagacctt catttttgag gacgaagtga
     1981 cgggtgggcg aatcccgaag aactacattc cgtcgatcga gaagggcttc cgggcggtgc
     2041 tcggcaaggg ccccctggcc gagtaccccg tcgtgggact gaaagtcgtg gtcgaagacg
     2101 gctcgtacca cgaggtcgat tccagcgacc gggcgttcca gaccgcagcg gaaggctgct
     2161 tccgcgagca cttcaaggac accaagcccg cgctgctgga gccgatcatg aaggtcgaga
     2221 tcgagtgtcc ggagaatttc cagggcccga tcaccggcga tctgatcagc cgccgcggga
     2281 tgatcgtcaa caccgacatg cgggacggga tcacgaacat cctggccgaa gtgccgctcg
     2341 ccgaaacctt cggctacgcg accgacatcc gcagcatgtc gcaggggcaa ggcacgttca
     2401 cgatggaatt gctctgctac cgcaaagtcc ccgccagcgt gcaggaagac atcat
//
LOCUS       DASUGB010000492         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_610948, whole genome shotgun sequence.
ACCESSION   DASUGB010000492 DASUGB010000000
VERSION     DASUGB010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610948"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VFV87_09440"
     CDS             complement(<1..1641)
                     /locus_tag="VFV87_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5104021.1"
                     /translation="MEMEAGEILIRKGLLNRGQLEQSRNMANGHGDGTKLIEAAIQLG
                     FVSEENALKAVGEEVGIEFIDLADAEVDLSLLKGFPQKLIHRQSLFPIRRDHGQLVVA
                     TSNPFDLYPLDEVSAATGLSVMPILAARAEISKLIKKHLGVGSETVDGLVAAAADDDA
                     GLELLSDIETDGSELSEMAQEASVVRLVNEILLEAIESRASDVHIESQTSGIAIRYRI
                     DGMLQTQPVPPEINRFESAIVSRLKIMSRLNIAEKRLPQDGRIKLKVHGREIDVRVSV
                     IPMIHGEGLVMRILDKGAMKFDLRAIGMEQDTYESWRQLIDLPHGIMLVTGPTGSGKT
                     TTLYSSLIEINQPDVKIITTEDPVEYQLEGINQIQVHSKIGLTFGASLRSILRHDPDI
                     VLVGEIRDLETAENAIQASLTGHLVFSTLHTNDAASAYTRLVDMGVEPFLVASTVEAV
                     MAQRLVRRLCSHCRQPYEPKKDDVPGDFPWDKKSGPVYKAVGCRDCRNTGYRGRMGIY
                     ELMVTTNEIRQLAHDRASSWKIMQCAWEQGMRSLRHDGW"
     gene            complement(1881..>2662)
                     /locus_tag="VFV87_09445"
     CDS             complement(1881..>2662)
                     /locus_tag="VFV87_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104022.1"
                     /translation="AIALVACIGVTAVATAQPGRGPGGGGFGFGGGAGNNLGLDLLND
                     ENVRNELGLVDDQVTKLREIGNSLREEAQAAFQDSGIDFRELFQLSEEERNAKLAPMR
                     EKMEKVAADAQKEIDQILLPQQRQRLKEIVVQSQIRRQGTSAALSSGTLAEELNITEE
                     QKEKLREMQQKVEEELRKEIEQLRIESRDKILSVLTAEQQAKLKSMMGSPIEFQQFGG
                     FGGGGGFGGRGPGGPGGPGGRGPGGQGGAGGGRFQRAPDGN"
BASE COUNT          450 a          821 c          827 g          564 t
ORIGIN      
        1 ccagccgtcg tgccgcaggg agcgcattcc ctgctcccag gcgcattgca tgattttcca
       61 ggagctggcc cggtcgtggg cgagctggcg gatttcgttg gtcgtgacca tcagctcgta
      121 aatgcccatc cggccgcggt agcccgtgtt gcggcaatcg cggcagccga cggccttata
      181 aacggggccg gatttcttgt cccagggaaa atcgcccggc acatcatcct tttttggctc
      241 gtagggctgg cggcaatggc tacagaggcg gcggacgagg cgctgggcca tgaccgcttc
      301 caccgtgctg gcgacgagga agggctcgac acccatatcg accaaacgcg tataggcgct
      361 ggcggcatcg ttggtgtgca acgtgctgaa gaccaggtgg ccggtgagcg acgcctggat
      421 cgcattttcg gccgtttcca gatcgcggat ttcgccgacg agaacgatgt ccgggtcgtg
      481 gcgcaggatg ctgcggagcg aagcgccgaa cgtcaggccg atcttcgaat gcacctgaat
      541 ctggttgatg ccttcgagct ggtattcgac cgggtcttcg gtggtgatga ttttgacgtc
      601 cggctggttg atctcgatca gcgagctgta gagcgtggtc gtcttgccgg agcccgttgg
      661 gcccgtgacg agcatgatgc cgtgcggcag gtcgatgagt tgtcgccacg attcataagt
      721 gtcctgctcc attccgattg cgcggaggtc gaacttcatc gcccctttgt cgaggatacg
      781 catgacgagg ccttcgccat ggatcatcgg aatgaccgac acgcggacgt cgatctcgcg
      841 gccgtgcacc ttgagcttga tgcggccgtc ctgcggcagg cgcttctcgg caatgttcag
      901 ccgcgacata atcttgaggc ggctgacgat ggcggattcg aagcggttga tttccggcgg
      961 gacgggctgc gtttgcagca tgccgtcaat gcggtagcgg atggcgatgc cggaggtctg
     1021 cgactcgatg tggacgtcgc tggctcgcga ctcgatggct tcgagcaaga tttcgttgac
     1081 aaggcgaacg actgacgctt cctgcgccat ctccgacagc tcggagccat cggtttcgat
     1141 gtcgctcagc aattccaggc cagcatcgtc gtcagctgcg gcggcgacca ggccatcgac
     1201 cgtttcgctg ccgacgccga ggtgcttctt gatgagcttc gagatctcgg cccgggccgc
     1261 cagaattggc atgaccgaaa ggccggtcgc ggcactgact tcgtcgagcg gatacaggtc
     1321 gaacgggttg cttgtcgcga cgacgagttg cccgtggtcg cggcggatcg gaaagagcga
     1381 ctgccggtgg ataagctttt gcggaaagcc cttcagcagc gagagatcga cctcggcatc
     1441 ggcgagatcg atgaattcga tgccgacttc ctcacccacc gctttgaggg cattctcttc
     1501 gctcacaaag ccgagctgaa tcgccgcctc gatcagcttg gtgccgtcgc cgtggccgtt
     1561 ggccatgttg cgcgactgct cgagctgacc ccggttgagg agtcccttgc gaatcaggat
     1621 ttcaccggct tccatttcga tcttttctgg cttacgaggc ggggccaggc tgcgagaaac
     1681 gagcggcagg cactttcgat ccgatgaact gcgaggaggc ggcgctgtgg agggaactac
     1741 ccgcgccgag cgaatgtagt ttcccgcccc gaggcagcag gcaagcgccc cggtgcgtcc
     1801 ccagcgatat cctgcgatcc aacgattgta tcatgttgcg aggacgctac tgcggccccg
     1861 ccaggtcatc cgcgtgaagc ttagttgccg tctggcgccc gctggaagcg accgccgcca
     1921 gcgccacctt ggccgccagg acctcggccg ccgggcccac caggacctcc cggtccacga
     1981 ccaccgaatc caccgccgcc gccaaagccg ccaaactgct ggaactcaat tggcgagccc
     2041 atcatgctct tcagcttggc ttgctgttcg gctgtgagca cgctcaggat cttgtcgcgc
     2101 gattcgatgc gcagctgctc gatctccttg cgaagttctt cttccacctt ctgctgcatc
     2161 tcacggagct tctctttctg ctcctcggtg atgttcaatt cctcggccag cgtgccgctg
     2221 ctcagggcgg ccgatgtgcc ttgccggcga atctgtgact gaacgacgat ttctttcagg
     2281 cgctggcgct gctgcggcag cagaatctga tcaatttctt tctgggcatc cgcggcgact
     2341 ttctccatct tttcgcgcat cggcgccaac ttggcattgc gctcttcttc cgaaagctgg
     2401 aagagttccc ggaagtcgat gccgctgtcc tggaacgctg cctgggcttc ttcacgcagg
     2461 ctgttgccga tctcacgaag ctttgtcacc tggtcatcga cgaggcccaa ctcattgcgg
     2521 acgttttcgt cgttcagcaa atcgagaccc agattgttcc ccgcgccgcc gccgaatcca
     2581 aatcctccac cgccaggacc gcggcccggc tgggcagtgg caacggccgt gactcctatg
     2641 caggcgacca acgcgatcgc ca
//
LOCUS       DASUGB010000493         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_890605, whole genome shotgun sequence.
ACCESSION   DASUGB010000493 DASUGB010000000
VERSION     DASUGB010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890605"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFV87_09450"
     CDS             <1..209
                     /locus_tag="VFV87_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104023.1"
                     /translation="AGGGIRGGVVHGSSDKLGAYPQDGLVKPEDITATILHCLGIDPN
                     TEVHDSQGRPFALSRGQVLHPILS"
     gene            complement(367..729)
                     /locus_tag="VFV87_09455"
     CDS             complement(367..729)
                     /locus_tag="VFV87_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104024.1"
                     /translation="MATLIPAPKIIQAAGNKPKVIEEFIGRVNSQTEAVSIARMRSPG
                     GWIEPGQAPEFDEYTVVLRGLVRVTTKSGTFDVAAGQAIIARAGEWVQYSTPGEGGAE
                     YIAVCLPAFAPGIVHRDA"
     gene            complement(744..1145)
                     /locus_tag="VFV87_09460"
     CDS             complement(744..1145)
                     /locus_tag="VFV87_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104025.1"
                     /translation="MSPVFLDTVGLLALWDEADQWHADAARAFAAMKQSRCSLVTSEG
                     VLLECGNAAARRPYREEVILLRTRMVERGEVVAPTAEELELAWEAYRLKDGAGAGIVD
                     QISFLLMRRLQLTRAFTNDAHFRAAGFETLF"
     gene            complement(1142..1351)
                     /locus_tag="VFV87_09465"
     CDS             complement(1142..1351)
                     /locus_tag="VFV87_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HEX5104026.1"
                     /translation="MTVQAVFENGVFRPVQPVAMPEKTLVEFEPRVVAGLPAPPPLDA
                     IYDILDRRFNSGQSDVAERHNEHQP"
     gene            complement(1463..>2008)
                     /locus_tag="VFV87_09470"
     CDS             complement(1463..>2008)
                     /locus_tag="VFV87_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104027.1"
                     /translation="PDLPYAQDIAYMNEMPTMKEWRRLAAADKLQGPAAIFFQPTKPV
                     EELYDCVADPHEIHNLAGDPKFAADLARLRAAHETWRETTGDLGLIPEAELMQQRRPG
                     GKYAVTAAPEIAIESGKATIHCATEGASIAYRVNKDIPAGDGRRWLLYTGPFTVMAGE
                     AIQAKSCRLGYRDSEIVGHTN"
BASE COUNT          358 a          684 c          593 g          373 t
ORIGIN      
        1 tggccggcgg cggtattcga ggcggcgtcg tccacggctc gtccgacaag ctgggcgcct
       61 atccccagga cggcctcgtc aagcccgagg acatcacggc gaccatcctg cattgcctcg
      121 gaatcgaccc caacaccgaa gtccacgatt cccagggccg acccttcgcc ctcagccgcg
      181 gccaggtgct gcacccgatc ttgtcctaag cgtagggtgg gacaagctcg cggccagttt
      241 gccctgcact gcgcgctgct tcgtccgcga gcgcaggccc accacccgcg acaaactctg
      301 gtgggccggc gctcgcgatg agtacgcaag ggagcagagc accagctggc gcgagcttgt
      361 cccaccctac gcatcgcgat gcacaatccc cggcgcaaac gccggcaggc agaccgcgat
      421 gtattccgcc ccgccctcgc cgggcgtgct gtactgcacc cattcgccgg cgcgggcgat
      481 gatcgcttgc ccggcggcga cgtcgaatgt gccgctcttg gtcgtcaccc gcaccaggcc
      541 ccggaggacg accgtgtact cgtcgaactc cggcgcctga cccggctcga tccagccgcc
      601 ggggctccgc atccgggcga tgctcaccgc ttcagtctgc gaattcacgc ggccgatgaa
      661 ctcctcaatc accttcggct tgttgccggc ggcctggatg atcttggggg cgggaatgag
      721 agttgccatg gaacgacact ggattaaaac agagtctcga acccagcggc gcggaaatga
      781 gcgtcgttgg tgaacgctct cgtcaactgc aggcgtcgca taagtaagaa cgagatctga
      841 tcaacgatac ctgcaccggc gccatccttc aggcggtagg cttcccacgc caattcgagt
      901 tcctcggcgg ttggcgcaac tacctcgcca cgttccacca tcctggtccg cagcaagatc
      961 acttcctcgc gatagggtcg ccgtgctgcg gcgttcccac attccaaaag aaccccctcc
     1021 gacgtgacga gtgaacagcg cgactgcttc attgcggcaa atgcccgcgc cgcgtccgcg
     1081 tgccattgat cggcttcatc ccagagggcc agcaacccaa ccgtgtccag aaaaaccgga
     1141 ctcatggctg atgctcgttg tggcgctcgg cgacgtcact ttggcccgaa ttgaatcgcc
     1201 gatccagaat gtcataaatg gcatccagag gcggcggtgc agggagtcca gcaacgacgc
     1261 gcggctcgaa ttcgacgagc gtcttttcag gcatcgcgac aggctgcaca gggcgaaaga
     1321 cgccgttctc gaacacggct tgaactgtca tgagggtctc tccacaaaag cgttcctctt
     1381 cccatcatat ccgctcacgc ctgacttccg ccaagtcggc acccaaatgg attccagtca
     1441 ccggaagaat ttgccctctg attcagtttg tatggcccac aatctcgctg tcgcgataac
     1501 cgagccggca agacttcgct tgtatcgcct cgcccgccat gacggtgaat ggccccgtat
     1561 acagcagcca tcgccggcca tcgcccgctg gaatatcttt gttgacccgg tatgcgatcg
     1621 acgcaccctc ggtcgcacag tggatcgtcg ccttgccgct ttcgatggcg atctccggcg
     1681 cggcggtcac ggcatacttg ccgccgggac gccgctgctg catcagctcg gcttcgggga
     1741 tcaggcccag gtcgccggtc gtttcccgcc acgtctcatg agctgctcgc agccgggcga
     1801 gatcggcggc gaatttcggg tcgccggcca ggttgtggat ctcgtgcgga tcggcgacgc
     1861 agtcgtacag ctcctcgacc ggcttggtgg gctgaaagaa gatcgccgcc ggcccctgga
     1921 gcttgtccgc tgccgccagc cgccgccatt ccttcatcgt gggcatctcg ttcatgtagg
     1981 cgatgtcctg ggcgtacggc agatcggg
//
LOCUS       DASUGB010000494         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1585679, whole genome shotgun sequence.
ACCESSION   DASUGB010000494 DASUGB010000000
VERSION     DASUGB010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585679"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFV87_09475"
     CDS             complement(<1..1049)
                     /locus_tag="VFV87_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104028.1"
                     /translation="MNDSSNGPSRRDFLKTSGVALTAGALAGAVVPRVHAAEDSTVQI
                     ALVGCGGRGTGAATNALSVQNGPVKLVAMADVFDHRLKNSHDALKGRFEKQMDVPEDR
                     KFISFEGYKSAIDCLRPGDVAIFATPPAFRWVHFKYAIEKGVNVFMEKPVTVDGPSTR
                     KMLALGEEAKKKNLKVAVGLMCRHCKARAELFDRIQNGELGDLIALRCYRMHGPVGSA
                     FSGPKPADENELMFQIRRFHSFLWASGGCYSDFYIHNIDECCWMKNDWPVEAQASGGR
                     QYHNYASPPGQSDIDQNFDTYNVEYTFKDGSKLFLNGRTMIGCHDEFASYAHGSKGLG
                     VISQSGHSPAHCRIY"
     gene            1462..1638
                     /locus_tag="VFV87_09480"
     CDS             1462..1638
                     /locus_tag="VFV87_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104029.1"
                     /translation="MAALPVVYFIAFLVLLQPYVFNAIGGVARAPAYRIRGQVFKSIF
                     KPINDIDQAIRPGF"
     gene            1841..2239
                     /locus_tag="VFV87_09485"
     CDS             1841..2239
                     /locus_tag="VFV87_09485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5104030.1"
                     /translation="MPTEFLNPPGLCPTFGWSHVVTTSGGKTIHISGQVGIDAEGNLV
                     GPGDLKRQTEQAFENLNTALAAAGATFEHVVKSTIFVVNFKPEMLSVIREVRGRYFSR
                     EHPPASTLVGVTALAGAAWLIEIEATAVVV"
     gene            complement(2634..>2853)
                     /locus_tag="VFV87_09490"
     CDS             complement(2634..>2853)
                     /locus_tag="VFV87_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5104031.1"
                     /translation="DGATAKTRFEAVNRGWVGITRPWHLLTTNTGSGYPHNASPEKAH
                     RLMEVLVERLATFLVELSQSKLDDRFPY"
BASE COUNT          518 a          861 c          863 g          611 t
ORIGIN      
        1 ttgtagatgc ggcagtgggc ggggctgtgg ccggactgcg aaattacgcc cagtcccttg
       61 ctgccgtggg catagctggc gaattcgtcg tggcagccga tcatcgtgcg gccgttcagg
      121 aacagtttgc tgccatcctt gaaggtgtat tcgacgttgt acgtgtcgaa attctgatcg
      181 atgtcgctct ggccgggcgg gctggcgtaa ttgtgatact gccggccgcc gctggcctgg
      241 gcttcgacgg gccaatcgtt cttcatccag cagcactcat cgatgttgtg aatgtagaag
      301 tcgctatagc agccgccgct ggcccatagg aagctgtgga agcggcgaat ctggaacatc
      361 agctcgttct cgtccgccgg cttgggtccg gaaaacgccg agccgaccgg cccgtgcatc
      421 cggtagcagc gcagtgcgat caggtcgccc agttcgccgt tctggatgcg atcgaacaac
      481 tccgcccggg ccttgcagtg acggcacatc aggccgaccg ccaccttcaa attcttcttc
      541 ttcgcctctt cgcccagggc cagcatcttc cgtgtgctgg ggccatccac cgtcaccggc
      601 ttctccatga agacgttcac tcctttctcg atggcatatt tgaaatgcac ccagcgaaac
      661 gctggcggtg tggcgaagat agccacatcg cccggccgca ggcagtcgat cgcgctctta
      721 tagccttcga agctgatgaa cttgcggtcc tcgggcacgt ccatttgctt ctcgaagcgc
      781 cctttgagtg catcgtggct gttcttcagg cggtggtcga agacatcggc catcgccacc
      841 agcttgaccg gcccattctg gacggagagc gcattggtcg ccgctcccgt tccgcggccg
      901 ccgcagccaa cgagggcaat ctgcacagtg ctatcctccg ccgcgtggac cctgggcaca
      961 accgccccgg ccagagcgcc cgcggtcagg gccacgccgg aggttttgag gaaatcgcgc
     1021 ctggaggggc cgtttgacga atcgttcatg atgacctggt ggggttagag aggaactgac
     1081 aataccttcc attgcgttat tcacagaata cgggcggcga ctgacctttt ctagccagtc
     1141 ggcctcggct gacagccgtt tctcacctgc cgcaacgctg cgattggcgg ccggtcggac
     1201 gccaccatcg atgcaggagg ggcaatgcgt gattcgaaac gccctggtcg cgagtgaaga
     1261 cgcacggatg gattcccgat ctcagacgtg tagaggagcc agcggagcgc gacccttcat
     1321 acttcccgga gactctcact ccatccctct ggctcggcgt ctcgtcagta gctatcgccg
     1381 ccagcgcaga aggcattgct tcttcgtgcg cggtagaatg gccgggcatg agttacgcgg
     1441 tgctataatc cgatcatctc ggtggcggca ctgcccgtcg tttacttcat cgccttcctc
     1501 gtattgctcc agccatacgt cttcaacgcc attggcggcg tggcccgcgc tcccgcatat
     1561 cgaatacgtg gtcaagtatt caaatcaatc ttcaagccga ttaacgacat cgatcaggcc
     1621 attcgaccgg gattttgata tcggcccggg ccctacgatc cagactttgc tccccggaga
     1681 cgcgacccat agttgccgcc actgaaacaa atatccggcg ccgatgacat gagacgttcg
     1741 atgttccgct tttcggcagg ccgctttcgt acggttcgca tcggcggtta tcgagcgcga
     1801 attcgtcctg catccacgcg aagaatctgg agccagcccc atgccgaccg agtttctcaa
     1861 tccgcccgga ctttgcccaa cctttggctg gtcgcacgtc gttaccacga gcggcggcaa
     1921 gacgattcat atctcgggcc aggtggggat cgacgcggaa ggcaatctcg tcggcccggg
     1981 cgatctcaag cggcagaccg agcaggcatt cgagaacttg aatactgcct tggccgctgc
     2041 cggcgcgacg ttcgagcacg tcgtcaagag cacgatcttt gtcgtgaact tcaagcccga
     2101 gatgctgtcc gtaattcgcg aggtgcgcgg ccgctatttc tcgcgcgagc acccgccggc
     2161 cagcacgctg gtcggagtca cggccttagc cggcgcggcg tggctgattg aaatcgaagc
     2221 gacggctgtg gtggtttagc ggcgaaatat gagcggcaac cgcacttttt gggcatcctt
     2281 gtttcagacc cctggccggc gtgcgatttc ggtacttcgc aatcctcctc gttgggattc
     2341 catcgattgg ccagggcatc acgtgcccga tacgccggga gtggaggaac catgtttcaa
     2401 tcgggtggaa atggccttcc ttgccgtcgc gttcattgtg gcttggcgct tccctcggcc
     2461 cttcgtcgac ctgccagagc ttgagggagt gtgcatctcg tgggtggccg gctcgccggg
     2521 caaatcgacg aggcaggccg taaactcaag cggttctgcc cgaaaatcaa gagttgagga
     2581 agcgcgaggt tcggaaagtt cacatggttc tcaacgtgaa aaccgagttt ttatcaatac
     2641 ggaaaccgat catccagttt cgactgcgag agctcgacca ggaacgtcgc cagccgttcg
     2701 acgaggactt ccatcaaccg gtgcgccttc tcgggcgagg cgttgtgcgg gtagccgctg
     2761 ccggtgttcg tggtcaggag gtgccaaggg cgggtaatgc cgacccagcc gcggttgacg
     2821 gcttcgaagc gggttttggc ggtggcgcca tcg
//
LOCUS       DASUGB010000495         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1585780, whole genome shotgun sequence.
ACCESSION   DASUGB010000495 DASUGB010000000
VERSION     DASUGB010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585780"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VFV87_09495"
     CDS             <1..1233
                     /locus_tag="VFV87_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104032.1"
                     /translation="NKYADKTIEESPYKATLESPYLKKNLREVIEGLHKVHPKIYPMQ
                     VGYRKFGNSGLEISDWWQTLGELCADDLCLVRSMWTTDNNHGAQLQFHTGRHSLEGQF
                     PTIGSWVHYGLGSLNDNLPQFVVLGTPIADCCGGAGAHGANYLGPRHSGIRLAVDPQN
                     PLPFAAPDNSTFQEEQVREFELLGKLNRLAAADYPDDPALAARIKSYELAYEMQAAVP
                     DVVNFAAESAETQRLYGLDQPATKTFGGQCLAARRMVERGVRFVQLFHGSNGGAGAWD
                     AHGNLKDGHSKLCGQVDRPIAALIHDLKRRGLLSETLVVIGTEFGRTPGAQGSNGRDH
                     HPYGFSVALAGGGIKGGIPHGATDELGFHAVENRHYVTDLHATLLHQLGLNPRQLEIP
                     GRKRLEIDFGEPIREIVA"
     gene            complement(1255..2019)
                     /locus_tag="VFV87_09500"
     CDS             complement(1255..2019)
                     /locus_tag="VFV87_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEX5104033.1"
                     /translation="MSDAADSASQPAADPPVSARNPWHRAVRIFVTILLVYFVVAYLL
                     MPLLWRRFAHRHPAIDDTPGITLTGDKHPGDPINVALIGSEEQVKQIMQAAGWFPADP
                     LGLRSDVKIAADTVLKRPYDDAPVSNLYLFGRKEDLAFEQPVGDDPRQRHHVRFWKSA
                     KVDDEGRPLWAGSATYDKRVGLSHTTGQVTHHISGDVDAERDHLFADLQKTGKLIDVQ
                     FIDDFHKVREGVNGGGDRWYTDGRLEVGTIRAAATR"
     gene            complement(2059..2316)
                     /locus_tag="VFV87_09505"
     CDS             complement(2059..2316)
                     /locus_tag="VFV87_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104034.1"
                     /translation="MSQNEHDHRGSEGSHDHKGEHGHTHRHEHAAPAKPRGLHRDWRL
                     WAAVAAALIAMAVYVLTVDESLSPFGWGPGAPADTPAADAP"
     gene            complement(2324..2686)
                     /locus_tag="VFV87_09510"
     CDS             complement(2324..2686)
                     /locus_tag="VFV87_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX5104035.1"
                     /translation="MIEVLPTGTDKVLGFKLSGTLHDPDYATFVPAIDAAVAKEGKVR
                     LLAQFHDFHGWDAKALWDDIKFSTTHCTKIERIALVGEKKWEQWMAKVCKPFTMAKIE
                     YFDVSKIDDAWKWLKEGL"
     gene            complement(2849..3052)
                     /locus_tag="VFV87_09515"
     CDS             complement(2849..3052)
                     /locus_tag="VFV87_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104036.1"
                     /translation="MSGKLPPTLPFALEASETFDIGSDTCTGVNDADYQPPFTLTAKL
                     NKLTIHMGRPQLEAAQHNNRASE"
     gene            complement(3196..>3868)
                     /locus_tag="VFV87_09520"
     CDS             complement(3196..>3868)
                     /locus_tag="VFV87_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5104037.1"
                     /translation="LTSLMKDNPPAKEDAPLIAKIAKIGLVPGKDFDASKLDDAGGVP
                     QAVQAKIMGHFKEAGTDEQGWVFTTRTGLYGTDYLQRSLITAIGLGANRPQDAVYPTS
                     QADAAGQPYSGERKYVMHFAKEQTPPVNAFWSLTMYDSKYFFVANPLDKYTVSPRNEL
                     QYNDDGSLDIYIQNESPGKDREANWLPAPQDKFILMLRLYWPKETNPSIIDGTWQPPG
                     VTAVK"
BASE COUNT          679 a         1175 c         1266 g          748 t
ORIGIN      
        1 aacaagtacg cggacaagac gatcgaggag tcgccgtata aggccacgct cgagtcgccg
       61 tacctgaaga aaaacctgcg ggaagtgatc gaggggctgc acaaggtgca tccgaagatt
      121 tacccgatgc aggtcggcta tcggaagttc ggcaacagcg gcctggagat cagtgattgg
      181 tggcagacgc tgggcgagct atgcgcggat gacctgtgcc ttgtccgcag catgtggacg
      241 accgacaaca accacggggc acagcttcag ttccacaccg gccgacattc gctggagggg
      301 cagtttccaa cgattggctc gtgggtgcat tacggcctgg ggtcgctgaa cgacaacctg
      361 ccgcagttcg tcgtgctggg gacgccaatc gccgattgct gcggcggcgc cggcgcgcat
      421 ggggcgaatt atctcggccc gcggcatagc ggcatccggc tggccgtcga tccgcagaat
      481 ccgctgccgt ttgccgcgcc ggacaacagc acgtttcagg aagagcaggt gcgcgaattt
      541 gagctgctcg gcaagctcaa tcgcctggcg gcggcggatt acccggatga cccggctttg
      601 gcggcgcgaa tcaagtcgta tgagctggcc tatgaaatgc aggcggccgt gccagacgtc
      661 gtcaatttcg ccgccgaatc tgctgaaacg cagcggctct acggactcga ccagccggcg
      721 acgaagactt tcggcggcca gtgcctggcg gcgcggcgga tggtcgagcg gggcgtgcgg
      781 ttcgtgcagc tcttccacgg cagcaacggc ggcgcaggag cgtgggacgc acacggcaac
      841 ctgaaggacg gccacagcaa attgtgcggt caggtcgatc ggccgatcgc cgcgctcatt
      901 cacgacctga aacgccgcgg gctcttaagc gagacgctcg tcgtgatcgg caccgaattc
      961 ggccgcacgc ccggagcgca gggctcgaac ggacgcgacc atcaccccta cggcttctcg
     1021 gtcgcgctgg ccggcggcgg gatcaagggc gggatccccc acggcgcgac ggacgagttg
     1081 ggttttcacg cggtggagaa ccgccactac gtcactgacc tgcacgcgac actcttgcac
     1141 cagctcggcc tcaacccgcg gcagcttgaa atccccggcc gcaagcggct ggagatcgac
     1201 tttggcgagc cgattcggga gatcgtcgca tagccgtgct gcgagctcaa aggctcagcg
     1261 cgttgcggcc gcgcggatcg tgccgacttc cagccggccg tcggtgtacc agcggtcgcc
     1321 gccgccattg acgccttcgc ggaccttgtg gaagtcatcg atgaactgca cgtcgatcaa
     1381 tttgccggtc ttttgcaaat cggcgaaaag gtggtcgcgc tcggcatcga cgtcgccgga
     1441 gatgtgatgt gtgacctggc ccgtggtgtg gctgaggccg acgcgcttat cgtacgtggc
     1501 ggaaccggcc cacagcgggc gcccctcgtc atcgaccttg gccgatttcc agaaccgcac
     1561 gtgatgccgc tggcggggat cgtcgccaac cggctgctcg aaggcgagat cctccttgcg
     1621 gccgaagaga tagaggttgc tgaccggggc gtcatcatac gggcgtttga ggacggtatc
     1681 cgccgcgatt ttcacgtcgc tccgcaggcc cagtggatcg gcggggaacc agccggcggc
     1741 ctgcatgatc tgcttgactt gctcctccga gccgatgaga gcgacgttga tcgggtcgcc
     1801 ggggtgcttg tcgccggtga gcgtgatgcc cggcgtgtcg tcgattgccg gatggcgatg
     1861 ggcgaaccgc cgccacagga gcggcatgag caggtacgcc acgacgaaat agacgagcag
     1921 gatcgttacg aagatccgca ctgcccggtg ccagggattg cgggcagaaa cggggggatc
     1981 ggcagcgggc tgcgaagcgg aatcggcggc gtcgctcata ggacggagcc ttgctggatc
     2041 ggtaaagtcg caggcgacct acggcgcatc cgcggccggc gtatcggctg gcgcgccggg
     2101 cccccagccg aacggagaca gcgattcatc gaccgtcagg acatagaccg ccatggcgat
     2161 gagcgccgcc gcgaccgccg cccacagccg ccaatcgcgg tgcaggccgc gcggtttagc
     2221 cggcgcggca tgctcatgac gatgcgtgtg gccgtgctca cccttatggt cgtgagagcc
     2281 ttcgctgccg cggtggtcgt gctcgttctg gctcatggcg cgactacaat ccttccttca
     2341 gccacttcca cgcgtcgtcg atcttcgaca cgtcgaaata ctcgatcttc gccatcgtga
     2401 acggcttgca gaccttagcc atccactgtt cccacttctt ctcgcccacc agggcaatgc
     2461 gctcgatctt cgtgcagtgc gtggtcgaga acttgatgtc gtcccaaagg gccttcgcgt
     2521 cccagccgtg gaagtcgtgg aactgggcca gcagacggac cttgccttcc ttggcgacgg
     2581 cggcgtcgat cgcggggacg aacgtcgcat agtccggatc gtgcagcgtg ccggaaagct
     2641 tgaacccgag gactttatcc gtgccggtcg gaagcacttc gatcatggcg attctcctgg
     2701 cgtcgcggag tagtaggcag tcatcctggt gacagacttc gcagtcctat tcctacacgg
     2761 attttcgcgc gcgtgccacg ccccactgcc gcttgctgaa ataggggccg tcgatgcggc
     2821 gaaacgttcc aaaatctctc tcgttgcctc actcgctcgc cctgttgtta tgctgggccg
     2881 cttcgagctg cgggcggccc atgtgaatcg tcagcttgtt tagcttggca gtgagggtaa
     2941 agggcggctg atagtcggcg tcgttcaccc cggtgcaggt gtcggagccg atgtcaaagg
     3001 tctcgcttgc ttcaagcgcg aacggcagtg tgggggggag ctttccactc acgacgtcct
     3061 tgccttccac gcaacaaagc catgagagtc gttggcgatg gcaacatggt cctttttttc
     3121 cgcaggggcc cgttttgccg tcaactcacc ggtgcgagcg aagcaacaac tccaggtgga
     3181 tggccgcgtg cgaaactact ttactgccgt cacccctggc ggctgccaag tgccgtcgat
     3241 gatcgaagga ttggtttcct tgggccaata caggcggagc atgaggatga atttgtcctg
     3301 cggtgcagga agccagtttg cttccctgtc tttgccgggc gactcgttct gaatgtagat
     3361 gtccaacgag ccgtcgtcgt tgtactggag ctcgttgcgc gggctgaccg tgtatttgtc
     3421 gagcgggttg gcgacgaaga aatacttgct gtcgtacatc gtcagcgacc agaaggcatt
     3481 gaccggcggc gtttgctcct tggcgaagtg catgacgtat ttgcgctcgc cgctgtaggg
     3541 ctggcccgcc gcatcggcct gggacgtcgg atagacggca tcctgcggcc gattggcgcc
     3601 gagtccaatg gccgtaatca gcgaccgctg caggtagtcg gttccataca gaccggtccg
     3661 agtcgtgaag acccaccctt gttcatcggt gccggcttcc ttgaaatgtc ccatgatctt
     3721 ggcttgcacc gcctgcggga cgcccccggc atcgtcgagt ttgcttgcgt caaagtcctt
     3781 gcccggcacg agcccgatct tcgctatttt ggcgatcagc ggcgcatcct ccttggcggg
     3841 cggattgtcc ttcatcagcg atgtcagc
//
LOCUS       DASUGB010000496         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1861983, whole genome shotgun sequence.
ACCESSION   DASUGB010000496 DASUGB010000000
VERSION     DASUGB010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861983"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFV87_09525"
     CDS             complement(<1..301)
                     /locus_tag="VFV87_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5104038.1"
                     /translation="MTARISDEVRERVAAHFQQKCTYCQSPLFLMPGAGTVEHIEPRS
                     AQGSDDESNLCLSCYRSNLRKATRIAAVDPRTRKKTPLFHPRQQTWQDHFRWSADP"
     gene            complement(298..678)
                     /locus_tag="VFV87_09530"
     CDS             complement(298..678)
                     /locus_tag="VFV87_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104039.1"
                     /translation="MTQLTFQLSDEIAQQVRDRAAQEGVPVEDFVASIVKRDVAPDPV
                     EELQRLAPLSDDEVLALADYMLSPEEDRRLQELLVQNRERLLTAEQGRELAELMALYN
                     EGMLKKAMGWAEAVRRKLREPIRS"
     gene            complement(927..4022)
                     /locus_tag="VFV87_09535"
     CDS             complement(927..4022)
                     /locus_tag="VFV87_09535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5104040.1"
                     /translation="MASLTASRSSVSARGAKGRQMTELSQGLKIDARFCPAEVADPFD
                     TVQWDLRSAQIKDENGKLMFEQKDAEIPSTWSQLATNVVVSKYFYGENGTPERERSVR
                     QLINRVTRTIADWGQADGYFAGDDDAERFYRELTWLCLHQHGAFNSPVWFNVGLFHMS
                     GVTGAKCNWRWNPETMDVEQPENPYEYPQGSACFIQSVEDNMEAIMSLATSEAMLFKF
                     GSGTGTDLSTLRSYREKLAGGGKPSGPLSFMRVYDQIAAVVKSGGKTRRAAKMQSLKV
                     WHPDILDFIECKWKEEKKAHCLIEKGGYEANFNGEAYSSILFQNANLSVRLTDEFMQA
                     VKNNQKWTTHWVTKPDVEGPSYEARWLLGRMSECAWQCGDPGVQYDTTINNWHTCPNS
                     GRINASNPCSEYMFLDNTACNLASINLMKFRKEDGTFDIDRFKAAARIFFIAQEILVD
                     HASYPTAEIARNSHLYRPLGLGYANLGSLLMSEGLPYDSEAGYSVCGSITALLHGTAN
                     LTSAEMAAVVGPFEGFHKNREPMLRVMQMHRDAVEKIGDEGPKYLKDAARELWDEVVA
                     LGKKVGYRNAQATVLAPTGTISFMMDCDTTGIEPDIALVKYKQLAGGGMLKIINNTAP
                     LALKKLGYDDDAIKAVSNYIGEHDTIEGAPQLKDEHLPVFDCAFKPANGIRSINWRAH
                     VRMMAAAQPFLSGAISKTVNMPSDVTPQDIADAYFWGWELGLKALAIYRDGSKQSQPL
                     NTKAEGDKAKESEKVVIVSKPRRERLPDTRNSVTHKFNVGGHEGYINVGMYDPEIPGG
                     QGRPGELFITMAKEGSTVGGMMDAFGTAISVALQYGVPLEDLVNKFSHMRFEPMGHTT
                     NPDIRIAKSVVDYIFRWMGIRFLAGYKEANSNVGKIPQAAVEDDDVSARSPAATKAGG
                     QPSLPRENKMLGATETKVKTAPPVTAEKSNGHSNGHGSSNGSNGTPPLPLGEGRGEGA
                     ASKSDAPALRIDGNGPAVRSEQFARFQLDAPSCDNCGAITVRNGNCYLCHNCGNSMGC
                     S"
     gene            4527..>4907
                     /locus_tag="VFV87_09540"
     CDS             4527..>4907
                     /locus_tag="VFV87_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5104041.1"
                     /translation="MPGKPLPRSFYARDPAIVARELVGKRLVRVTPEGLCGGRIVETE
                     AYLSSRDPACHANRGMTPRNAAMFGPAGHAYVYMIHAKWCFNCVTEDVGLGSAVLIRA
                     IEPLVGLALMHARRGVEDVLDLARG"
BASE COUNT          842 a         1501 c         1546 g         1018 t
ORIGIN      
        1 ctggatccgc ggaccagcga aagtggtctt gccacgtctg ttggcgagga tgaaacagag
       61 gcgttttctt tcgagtgcgt ggatcgacgg cagcgattcg ggtggccttg cgaagattgc
      121 tacggtaaca cgaaaggcag aggttcgatt cgtcgtcgct gccttgggcg gagcgcggct
      181 cgatgtgctc gacggtgcca gcgccgggca tcaagaagag aggcgactgg cagtaagtac
      241 acttttgctg aaagtgggca gcaacccgct cacggacttc gtcgctaatc ctggcggtca
      301 tgagcggatt ggctcacgga gcttgcgccg caccgcctcc gcccagccca tcgccttctt
      361 gagcattcct tcattgtaca aggccattaa ttcggccagc tctcggcctt gttcggctgt
      421 caacagacgt tcgcgattct gcaccaacaa ttcctgaagc cggcgatctt cctcgggcga
      481 gagcatgtag tcggcaagcg ccagcacctc atcgtcggaa agcggcgcca ggcgctgcag
      541 ctcttctacc ggatcgggcg ctacatcgcg cttgacgata ctggctacaa aatcctccac
      601 cggcacgccc tcctgggcag cccgatcacg gacttgctga gcgatttcgt cggagagttg
      661 aaacgtgagc tgggtcatca ccaaatcagc caagtgggat aaccaacaac ataccgcgcc
      721 gccatcgcag cccgcaaacg gccgaacgtc cccgagcggc gacgagtcgc cgcactccaa
      781 attacaaaac gggttgcccg gcggcgtcca tcccggctaa ttccttggcg ggcaaccctg
      841 tgtgcggagt gagtggtgag taggtgagtg gtgagttgtg gcaaccactt accactcaca
      901 actcaccact cacgggccga aggcccctac gaacatccca tgctgttacc gcagttgtgg
      961 caaaggtagc aattgccgtt gcggacggtg atggcgccgc agttgtcgca gctcggggcg
     1021 tcgagctgga agcgggcgaa ctgctcgctg cggaccgcgg ggccgttccc gtcgatgcgg
     1081 agggccggag cgtcactctt gctggcagcg ccctcacccc ggccctctcc cagagggaga
     1141 gggggagtgc cgttcgagcc attcgagctg ccgtgcccgt tggaatgccc gttcgacttc
     1201 tccgcagtga ccggcggggc ggttttcacc ttcgtttccg tcgctccgag catcttgttc
     1261 tcgcgcggga gagatggctg cccgccggcc ttcgtggcgg cgggcgagcg agctgagacg
     1321 tcgtcgtcct cgacggcagc ttgcggaatc ttgccgacat tgctgttcgc ctccttgtat
     1381 cctgcgagga agcggatgcc catccagcgg aagatgtaat cgacgacgct cttggcgatg
     1441 cggatgtcgg ggttggtcgt gtgacccatc ggctcgaacc gcatgtgcga gaacttgttg
     1501 accaggtcct cgagcggcac gccgtattgc agggcgacgg agatcgccgt gccgaaggcg
     1561 tccatcatgc cgccgacggt cgagccttcc ttggccatcg tgatgaacaa ttcgccgggc
     1621 cggccctgtc ctcccggaat ttccgggtcg tacatgccga cgttgatgta gccttcgtgc
     1681 ccgccgacgt tgaacttgtg cgtcaccgag ttccgcgtgt cgggcaggcg ctcgcgccgc
     1741 ggcttggaga cgatgacgac cttttcgctc tccttggcct tgtcgccctc ggccttcgta
     1801 ttgagcggct ggctttgctt gctgccgtcg cgatagatgg cgagggcttt gaggcccagc
     1861 tcccagcccc agaaatacgc gtcggcgatg tcctgcggcg tgacgtcgct gggcatgttg
     1921 accgtcttgc tgatcgctcc cgacaggaac ggctgggcgg cggccatcat gcggacgtgg
     1981 gcccgccagt tgatgctgcg gatgccgttg gccggcttga aggcacagtc gaagaccggc
     2041 aggtgctcgt ccttgagctg cggggcccct tcgatcgtgt cgtgctcgcc gatgtagttg
     2101 ctgaccgcct tgatggcatc gtcgtcgtag ccgagcttct tcagggccag cggggccgtg
     2161 ttgttgatga tcttcagcat gccgccgccg gcgagctgtt tgtattttac gagggcgatg
     2221 tccggctcga tgccggtggt gtcgcagtcc atcatgaagc tgatcgtgcc ggtcggggcg
     2281 aggaccgtcg cctgggcgtt gcgatagccg accttcttgc cgagggcgac gacttcgtcc
     2341 cacagctcgc gggcggcgtc cttgagatac ttcggcccct cgtcgccgat cttctcgacg
     2401 gcatcgcggt gcatctgcat cacgcggagc atcggctcgc ggttcttgtg gaagccctcg
     2461 aaggggccga ccacggcagc catttccgca ctggtcaggt tggccgtgcc gtgcagcaga
     2521 gcggtgatcg agccgcacac gctgtagccg gcctcgctgt cgtacggcag cccctcgctc
     2581 atcagcaggc tgccgaggtt ggcatagccc agaccgagcg ggcgatagag gtggctgttc
     2641 ctggcgatct ccgccgtcgg atagctggcg tgatcgacga gaatttcctg ggcgatgaag
     2701 aagatgcgtg ccgccgcctt gaagcggtcg atgtcgaacg tgccatcttc cttacggaac
     2761 ttcatcaggt tgatgctggc caggttgcaa gccgtgttgt caaggaacat atactcagag
     2821 cacgggttgc tggcgttgat ccggccgctg ttggggcagg tgtgccagtt gttgatcgtc
     2881 gtgtcgtact gcacgcccgg atcgccgcac tgccaggcgc attcggacat ccggccgagc
     2941 agccagcggg cttcgtagct ggggccttcg acatcgggct tggtgaccca gtgcgtggtc
     3001 cacttctgat tgttcttgac cgcctgcatg aactcgtcgg tcaggcggac cgagaggttg
     3061 gcgttctgga agaggatcga gctgtaggct tcgccgttga agttcgcctc atagccccct
     3121 ttctcgatca ggcagtgggc cttcttctct tccttccact tgcactcgat gaaatcgagg
     3181 atgtccgggt gccagacctt gagcgattgc attttcgctg cccgacgcgt cttgccgccg
     3241 ctcttcacga ccgcggcgat ctggtcataa acccgcatga acgacagcgg gccactgggc
     3301 ttgccgccgc cggccagctt ttcgcgatac gagcggagcg tggaaaggtc ggttcccgtg
     3361 ccgctgccga acttgaagag catggcctcg ctcgtcgcca ggctcatgat cgcttccata
     3421 ttgtcttcga ccgactggat aaagcaggcc gagccctgcg gatattcgta cgggttctcc
     3481 ggctgctcga cgtccatcgt ctcgggattc cagcgccagt tgcacttagc cccggtgacg
     3541 ccgctcatgt ggaacaagcc gacgttgaac caaacgggcg aattaaatgc cccgtgctgg
     3601 tgcaggcaga gccaggtcag ctcgcgatag aaccgctcgg cgtcgtcatc gccggcgaaa
     3661 tagccgtcgg cctggcccca gtcggcaatc gtccgcgtaa cgcggttgat gagctgccgc
     3721 acgctccgct cgcgttcggg agtgccgttt tcgccgtaaa agtacttgct gacgacgacg
     3781 ttcgtggcca actggctcca agtggagggg atttccgcgt ccttctgctc gaacatcagc
     3841 ttgccgttct catccttgat ctgggcactg cgcaggtccc actggacggt gtcgaacggg
     3901 tcggcgacct cggccgggca aaagcgggcg tcgatcttca ggccctggga aagctcggtc
     3961 atctggcgac ccttggcgcc gcgggcggaa acgctcgaac gagacgcggt cagactagcc
     4021 atcgtcagct ccttccagtg gaatgcgcag cggccatcgt gccgccgcat ccttgattat
     4081 gaacaaaaaa ctactgaaca gcgagcactt cggtcatggc cgcgactgcc ccgtcagggc
     4141 ccagaacagg cacccacccg cccgaccagc cttctacgca ggttcttggc tcgacatact
     4201 acatatagtg gttctcggca gtcgctacca caacctattg tgtgtcgcga gaatttaatg
     4261 atctcaggaa gcgagtcaac agccgcccag atcgtgtcgc aagtcgttgt atatcaatga
     4321 gttgcgatcg ttcttgccca tccccccacg catttgcaaa cgtattgtta aggcgcaaga
     4381 tattctaccg attggactta ggtaaatcca ggcggcagaa ggtgggccgc cgcaagcctc
     4441 gtccagaatc ttaaaatctt gtcgtggaag caccgctagc ggctttggcg gatagtgtac
     4501 gagatttctg ccttataatc gccgagatgc ccggaaagcc gctgccaagg tcgttttatg
     4561 cccgcgatcc ggccattgtc gcgcgcgagc tggtcggcaa gcgactggtg cgagtgacgc
     4621 cggaagggct gtgcggcggg cggattgtcg aaaccgaggc ctacctgagc agccgcgacc
     4681 cggcgtgcca cgcaaaccgc gggatgacgc cccgcaacgc ggcgatgttc ggcccggcag
     4741 gtcatgccta cgtctatatg atccacgcca agtggtgctt taactgcgtc acggaagatg
     4801 tgggcctggg cagcgccgtc ctgatccgcg cgattgaacc gctcgtcggc ctcgcgctga
     4861 tgcacgcccg ccgcggcgtg gaagatgttc ttgatctcgc ccgcggc
//
LOCUS       DASUGB010000497         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1724575, whole genome shotgun sequence.
ACCESSION   DASUGB010000497 DASUGB010000000
VERSION     DASUGB010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724575"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFV87_09545"
     CDS             <1..402
                     /locus_tag="VFV87_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5104042.1"
                     /translation="MFDNKPNETQYFYTDFDSVGESLSGGNSYSITFPKGDLPPVDGF
                     WSLTLYNDKHLFHPNDRKRYSLGTKNKGLKFNDDGSLTLYAGAKSPGLDKESNWLPAP
                     AGAFSLYIRAYWGRKGILDGSWKPPVVKKAG"
     gene            565..1866
                     /locus_tag="VFV87_09550"
     CDS             565..1866
                     /locus_tag="VFV87_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX5104043.1"
                     /translation="MDASLQKRAEQLATEFASQARTAEDLNGLIRLMMKSGLERMLNT
                     ELDVHLGRRPSAAGLAASPADESPAVPEPLPAALAKLPKRGPNRRNGKTPKTVQGDLG
                     ELTIDTPRDRDGTFEPVLIPKHQRRVPGFDEKILALYAKGMTTRDIQEIVKELYGVEV
                     SPTLISEITSDLDAEVTAWRTRSLDPVWPIVYFDGIVVHVRGANGRVSQHTVYVALGV
                     NLEGKKELLGLWLAETEGAKFWLTCLTDLQNRGLQDIFVACIDGLAGFAEAIHTAYPK
                     TSVQLCLVHLVRAALRYVSTQDSQAVARDLKKVYQAATVIEAEQMLTAFAQAWDAKYP
                     TIAKMWRAKWTDIITLFDYPPAIRKAIYTTNAIESVNSVIRKFTRNRKIYPSEESALK
                     NVYMAIREASARWTMPIHHWKQALNHFAILFEDRMPKTISN"
     gene            complement(1856..>2140)
                     /locus_tag="VFV87_09555"
     CDS             complement(1856..>2140)
                     /locus_tag="VFV87_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX5104044.1"
                     /translation="SPFPGMDPFLELNWPDVHAALITYARDQLQERLPPDLVARMESR
                     VVLEDEAGDELRTHRPDVSVVEQGRGGAAAAVLTGACNTFCVRAAGNVSC"
BASE COUNT          457 a          647 c          646 g          390 t
ORIGIN      
        1 atgttcgaca acaagccgaa cgagacccag tatttctaca ccgacttcga ttcggttggc
       61 gaatcgctca gcgggggcaa cagctattcg atcactttcc ccaaaggtga cttgccgccg
      121 gtcgatggct tctggtccct aacgctctac aacgacaagc acttatttca ccccaacgac
      181 cgcaaacgat attcgctcgg aaccaaaaac aagggcctca agttcaatga cgacggttcc
      241 ctgacgctct acgccggcgc gaagtcgccc ggcttggata aggaatcgaa ttggctgccc
      301 gcccccgccg gggcgttttc gctttacatc cgcgcatact ggggtcggaa gggcattctg
      361 gacggttctt ggaagccgcc ggtcgtcaag aaagccgggt gagccgctcg gctcaagtcc
      421 actcacgaat ccgcattgga gcagaaaaaa tgaaggcaat ctggaccatt tcgttcgcgg
      481 caatgcttgg ccctgtaaga aatcttgtgt cagctagcag aaacgggtag caatagtagc
      541 cctttctgaa atggagactg acgcatggat gcctcgttgc agaagcgagc ggagcagctc
      601 gcgacggagt ttgccagcca ggccaggacg gcggaagacc tcaacggcct gattcggctg
      661 atgatgaagt cgggcttgga gcggatgctc aataccgaac tggacgtgca tttgggccgg
      721 cggccctcgg cagccggcct agccgcttca ccggcggacg agtcgccggc ggttcccgag
      781 cctttaccag ccgccttagc caagctgccc aagcgcgggc cgaaccgtcg caatggcaag
      841 acgcccaaga cagtgcaagg cgacctgggc gagctgacca tcgacacgcc ccgcgaccgc
      901 gatggcacct tcgagccggt gctgattccc aagcatcagc ggcgggttcc cggcttcgac
      961 gagaagattc tggcgttgta cgccaaaggg atgacgaccc gcgacatcca agagatcgtg
     1021 aaagagctgt atggcgtaga ggtttctccc acgctgatct cggagatcac gtccgatctg
     1081 gacgcggaag tgacggcctg gcggacgcgc tcgctcgatc cggtttggcc gatcgtgtat
     1141 ttcgacggga tcgtcgtgca tgtgcgggga gccaacggcc gcgtgtcgca gcacacggtt
     1201 tacgtggctt taggcgtcaa tctggagggt aagaaggagc tgctgggcct gtggctggcc
     1261 gagacggaag gcgccaagtt ctggctgact tgtctgacgg acctgcagaa ccggggactc
     1321 caggacatct tcgtggcttg catcgacggc ctggccggct ttgccgaggc gattcacacg
     1381 gcttatccga aaaccagcgt gcagctctgc ctggtgcatc tggtgcgggc ggcgctgcgg
     1441 tacgtgagca cgcaggacag ccaggcggtc gcgcgggacc tgaagaaggt ctatcaggcg
     1501 gcgacggtga tcgaagccga gcagatgctc accgctttcg ctcaagcctg ggacgcgaag
     1561 tatccgacga ttgccaaaat gtggcgggcc aagtggaccg atatcattac cctgttcgac
     1621 tatccgccgg ccatccgcaa ggcgatctac accaccaacg ccatcgagtc ggtcaacagc
     1681 gtgattcgca agttcacgcg gaaccgcaag atctacccca gcgaggagtc cgccttaaag
     1741 aacgtgtaca tggcgattcg cgaggcgtcc gccaggtgga cgatgcccat ccaccactgg
     1801 aaacaagccc tcaaccactt cgccatcctg ttcgaagatc gaatgcccaa gaccatcagc
     1861 aactgacgtt cccggctgct ctgacacaaa aagtattaca ggcccccgtt agaacggccg
     1921 ctgccgcacc gccgcggcct tgctcgacca cgctgacatc tggacgatgc gtgcgcagtt
     1981 cgtcgccggc ctcatcttcg agtacgactc gggactccat ccgggcgacg agatccggcg
     2041 gaagacgctc ctgcagttgg tcgcgcgcat aggtaatcag cgctgcgtgg acatcgggcc
     2101 aattcagctc cagaaacggg tccattcccg gaaacgggct
//
LOCUS       DASUGB010000498         6767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_890821, whole genome shotgun sequence.
ACCESSION   DASUGB010000498 DASUGB010000000
VERSION     DASUGB010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890821"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2250)
                     /locus_tag="VFV87_09560"
     CDS             complement(145..2250)
                     /locus_tag="VFV87_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5104045.1"
                     /translation="MTTVATILGPSGKIAARLPHYEHRPQQLAMAEGVARALAEGHHL
                     VAEAGTGVGKSFAYLVPAIQYVTAKAPLAPGGRGAGGTDDADEAPRRRIIVSTHTISL
                     QEQLLLKDLPLLRSVIPEEFTAVLVKGRGNYLSLRRMNAAAERVASLFSQEEEVRDLR
                     QIIAWSKNTGDGSRSDLSFQPAWSVWDEVQSDSGNCLGRKCPTYKDCFYYRARRRVHN
                     AQILVVNHALFFSDLALRRIGASILPDYEAVIFDEAHTLEQVAGDHLGLSVTAGQVQY
                     ILNKLYNDRQNKGLLVTRKLRDAQDIAVECHTRADEFFDDVRAATKSAGQGRPHALRK
                     ASSPLSPRGRGAGGEGASGSLRVYQPDLIENRLSPALAQLARSVRQAASEIDGEGEKL
                     DFTSAHDRLMALAGELTHWVEQQSADTVYWVETAPARFGRSRVTLAAAPIDVGPVLRT
                     ELFEKTRTVVMTSATLAVGQPPSFEYFQSRLGLTSAETLRAGSPFNFREQAQLILVRG
                     LPDPTNEKARFERQSIELIKRYVERTDGHAFVLFTSYGSLRQAASELTPWLAARKLAL
                     YSQADGTPRGQLLDAFKANPRGVLLGTDSFWQGVDVPGDALTNVIITKLPFSVPDQPL
                     LEARLEAIRAAGGNPFRDYQLPAAVIKLRQGFGRLIRTARDRGIVVILDPRVKTKPYG
                     RVFLDSLPECTVVEESATD"
     gene            2457..3998
                     /locus_tag="VFV87_09565"
     CDS             2457..3998
                     /locus_tag="VFV87_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX5104046.1"
                     /translation="MPIEFPCTTCGQLVRTPESAAGKKGKCPHCASIVQIPAAGGQGT
                     GDRGQGAVERRRTEDRGQGTGSRGQGTGGGAPAAGGAVISFPCGSCGQTVRTPMSAAG
                     KKGKCPHCHAVVQIPGGPAPTTPPAAATVAGLTPLAPLPSQQRNPAAAPKPAPSGPWK
                     GTPTQQRPAPARPAAPPAEHVEELPTLTPIDSTPGLTPLPPQGAGLTPLGPAGGLTPL
                     GPAPAGGLTPLADPFGGLASMGGDPLAGLPAANISGRTLGPAAIPNPLGASPGYGGAA
                     LNPYASPAGGAAYSAGYSDFSDAVRKGLPWERRADMESFQDTATLILSEPQNAFMQMR
                     RTGGLMNSLAFWIVAAIIGQVANTVYGGILGMILLAIGNAPGEAYAALAVSVGLQLVG
                     GLIGTIIFAPVGAFIGAAIWHVVLLVFGCARGGFEATFRAQCFVGGAVVLLNIIPILG
                     PLIGFFYGIALFIHAFTHAHEVSGGRATAAVLTLYGLLCCCISPLIIFPLMGLIARLG
                     AGGPF"
     gene            4023..5093
                     /locus_tag="VFV87_09570"
     CDS             4023..5093
                     /locus_tag="VFV87_09570"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX5104047.1"
                     /translation="MTIEFRCSQCNQLLRVPDTSAGKNARCPKCQSLMQVPSAEGSGA
                     SPPPLPPPFAASGSGGMPSFGPPPPASGAGDDPFSFLNKGGGGSSGGGATPPPAGPFG
                     NAGAAPGFVPSKPAPSANPYASPAGAAYSYQPMAPGPGYGNRPGLPWEVKGQSVSTWW
                     ETAKLCMTQPTYAFSVMRQYGGMGQPMLYCGYGLAAGMIGQMLWYLPLMGIMGLMMNQ
                     GGNGQDAAMFVGIQVVSSIFQSVIGVLIGATLMLLIGAAILHVCLMLVGGARQGFETT
                     LRVLGYAQGSIAWMNVIPIFGPLVGAIWGLVIEIIGVARAHEIETGKAVLAVFLPLIA
                     CCVLAGGLFALIMALGIAGANM"
     gene            complement(5109..5645)
                     /locus_tag="VFV87_09575"
     CDS             complement(5109..5645)
                     /locus_tag="VFV87_09575"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX5104048.1"
                     /translation="MKVRFSRWLAGAGLGLAMASLFVWSGSQAEDKKEEDAAVARTRK
                     QVQVLDDVYKAAVVLITEHYVNDADDLPAGSAAIALFDAIKKKGHHEVRLLDATGEPL
                     EDKNVAKDAFEKAAIAALKGGKPYYDQVIEKDGKRYLRAATPIPVVLKKCTMCHPNYE
                     NAKPGEPIGALGYTVPIE"
     gene            complement(5724..6224)
                     /locus_tag="VFV87_09580"
     CDS             complement(5724..6224)
                     /locus_tag="VFV87_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5104049.1"
                     /translation="MRLALHTDYALRTLLYLASNPGRASAAEVAEFYRISKDHIAKVV
                     QTLVRQGYVRSIRGIGGGIELAKRPEDIRVGQVILDCEGNMHLLDCVGIENVCVIQPG
                     CRLKTVLAKAERIQMEYLQSVKLSDLLMPGVTLLEIRQAGDKEEGTGNRGGRSKVKRP
                     RSKVRK"
BASE COUNT         1114 a         2137 c         2250 g         1266 t
ORIGIN      
        1 gttgatgcgt cttcagcacg cggtccatgt tgaggatcgc gatcggcagg gaatcgtcag
       61 cgcgggtaga ggacgtggca aacagagcga tcaaaaagag gcaaatgcct gagccgccgc
      121 atcttttacc cttcatattt cacctcagtc ggttgccgat tcctccacca ccgtacactc
      181 cggcagcgaa tcgaggaaca cacggccgta gggcttggtc ttcacccgcg gatcgaggat
      241 cacgacgatc ccgcggtcgc gggcggtgcg gatcagccgg ccaaatcctt gccgcagttt
      301 aatcaccgcg gcaggcagtt ggtaatcccg gaaggggttg ccccccgctg cgcggatcgc
      361 ctcgagccgg gcttccaaga gcggctgatc ggggacgctg aacggcagct tggtgatgat
      421 gacgttcgtg agggcgtcgc ccggcacatc gaccccctgc cagaagctgt cagtaccgag
      481 caagacgccc cgcggattgg ccttgaacgc gtccaggagc tgcccgcgcg gcgtgccgtc
      541 ggcctggcta taaagtgcca gctttcgcgc ggcgagccag ggcgtcaatt cactggccgc
      601 ctggcgcagc gaaccatagc tcgtgaagag cacgaacgcg tggccgtcgg tccgctcgac
      661 gtagcgtttg atgagctcga tcgactgccg ctcgaagcgg gccttttcgt tggtcggatc
      721 gggcaggccg cggacgagaa tgagctgcgc ctgctcgcgg aagttgaacg ggctgccggc
      781 gcggagcgtc tcggcactcg tcagcccaag ccgcgactga aagtattcga acgacggcgg
      841 ctggccaacg gcgagcgtgg cgctggtcat cacgacggtg cgggtcttct cgaagagctc
      901 ggttcgcagc acggggccga cgtcaatcgg tgcggcggcc agcgtcactc gcgagcggcc
      961 gaagcgggcc ggggccgttt cgacccaata gacggtgtcc gcgctctgct gctccaccca
     1021 gtgcgtcagc tcgccggcca gcgccatcag ccggtcgtgg gcggaggtga agtcgagctt
     1081 ttcgccttcg ccgtcgatct cgctggcggc ctgtcggaca gagcgtgcca gttgggcgag
     1141 ggccgggctc aagcggtttt cgataaggtc gggctgatag acgcggagcg agccactcgc
     1201 cccctcaccc ccggcccctc tcccacgagg ggagagggga gaagaagcct ttcgcagagc
     1261 atgaggtctg ccctgtccag cgctcttggt tgctgctcgc acgtcgtcga agaactcgtc
     1321 ggcccgtgtg tgacattcga cggcgatgtc ctgggcgtcg cgcagcttgc gcgtcacgag
     1381 cagccccttg ttctgacggt cgttgtagag cttgttcaga atgtactgta cctggccggc
     1441 ggtgacgctc aggccgaggt gatcgccggc gacctgctcc agcgtgtggg cctcgtcaaa
     1501 gatcaccgct tcgtagtcgg gcaggatgct cgcgccgatc cgccgcaggg cgaggtcgct
     1561 gaagaagaga gcgtgattga cgaccaggat ctgggcgttg tgaacgcgcc ggcgggcgcg
     1621 gtagtaaaag cagtccttgt acgtcgggca tttgcggccg aggcagttgc cgctatcgct
     1681 ttgcacttcg tcccacacgc tccaggccgg ctggaatgag agatcgctcc gcgagccgtc
     1741 gccggtgttt ttggaccagg cgatgatctg ccgcagatcg cggacttctt cctcctgcga
     1801 gaacaggctg gcgacccgct cggcagcggc gttcatccgg cgcaggctga gatagttgcc
     1861 acggcctttg acaaggacgg cggtgaactc ttccgggatc acgctccgca aaagtggcag
     1921 gtctttgagc aggagctgct cctgcaggct gatggtgtgc gtcgaaacga tgatccggcg
     1981 gcggggggcc tcgtcggcat cgtctgtccc cccagcccct ctcccaccag gggcgagggg
     2041 agcctttgcg gtcacgtact gaatcgccgg caccaggtag gcaaagctct tgccgacgcc
     2101 cgtgccagcc tcggccacga ggtggtgtcc ctcagccaat gcgcgggcca ctccctcggc
     2161 catcgcgagc tgctgcgggc gatgctcgta gtgcggcagg cgggcggcga tcttgccgct
     2221 cgggccgaga atggtcgcga cggtggtcac aagcgtcacg atagcagatt gtagggtgca
     2281 taagcccgcg gcgggcgcaa tgcaccgcgc gcctggccac tggcagcgcc tggtgcattg
     2341 cgctgcgctt atgcacccta caggctacag actggcaaga tcggcaatta gaatgcgaac
     2401 attctggcta gagccgggtt cccgccttcg cccactttgc cgcttgcgcg cttccaatgc
     2461 cgatcgagtt cccttgtacc acctgtggcc agcttgtccg cacgcccgaa tcggcggcgg
     2521 gcaagaaggg gaagtgtccg cattgtgcgt cgattgtgca gattccggcg gccgggggac
     2581 agggaacagg ggacagggga cagggggcag tagagagacg gaggacagaa gacagaggac
     2641 aggggacagg gagcagggga cagggaacag ggggaggcgc gccggctgct ggcggggcgg
     2701 tgatttcgtt tccctgtggc tcgtgcgggc agacggtgcg gacgccgatg agcgcggcgg
     2761 gaaagaaagg caagtgcccg cattgccacg cggtggtgca aattcccggc gggccggctc
     2821 cgacgacgcc acctgccgca gcgaccgtcg ccggcctcac gccgttggcg cccttgccgt
     2881 cgcagcaaag gaacccagcg gccgccccca agccagcccc cagcggcccc tggaagggca
     2941 ctccaacgca gcaaaggccg gcgccggcca ggccagccgc accgcccgcg gagcacgtcg
     3001 aggaattgcc gacgctgacg ccgatcgact ccacgccggg cctgactccc ttgccgccgc
     3061 aaggcgccgg cttgaccccg ctggggccgg ctggaggttt gacgccgctc ggccccgcgc
     3121 cagccggcgg cttgacgccg ctggccgatc cgttcggcgg cttggcctcg atgggcggcg
     3181 atcccctggc gggcctgccg gcagcgaaca tcagcggccg cacgctggga ccagcggcga
     3241 ttcccaaccc gctcggcgcc tcgcccggat atggaggcgc ggcgctcaat ccctacgcct
     3301 cgcccgccgg cggcgcagcg tattcggcgg gctactccga cttcagcgac gccgtccgca
     3361 agggtttacc gtgggagcgc cgagcggaca tggagtcgtt ccaggacacc gccacgctga
     3421 tcctcagcga gccacaaaac gcgttcatgc agatgcggcg cacgggcggg ctgatgaact
     3481 cgctggcgtt ctggatcgta gcggcgatca tcggccaggt ggcgaacacg gtgtatggcg
     3541 gcatcctggg aatgatcctg ctggccatcg gcaatgcgcc gggcgaggcc tacgcggcgc
     3601 tcgccgtttc ggtcggcttg cagctcgtgg gcggcctgat cgggacgatc atttttgctc
     3661 cggtcggcgc gtttattggc gcagcgatct ggcacgtcgt gctgttggtc ttcggctgtg
     3721 cccgcggcgg gttcgaggcg acatttcgcg cccagtgctt cgtgggcgga gcggtggtcc
     3781 tgctgaacat cattccgatc ctgggcccct tgatcggctt cttttatgga atcgccttgt
     3841 tcatccacgc ttttacgcac gctcacgagg tctcgggtgg acgcgcgacg gctgccgtgc
     3901 ttacgctgta cggcttgctt tgctgctgca tttcgccgct gatcattttt ccgctcatgg
     3961 gcctcatcgc gcggctgggc gcgggcggac cgttttagcc atcttctgag aatgcctttt
     4021 ccatgaccat cgaattccgc tgcagtcagt gcaatcaact cctccgcgtg ccggacacca
     4081 gcgccggcaa gaacgcccgc tgcccgaagt gccagtcgct gatgcaggtg ccatcggcgg
     4141 agggttccgg cgcatcgccg ccgcccctac ctccgccgtt tgcggcaagt ggctccggcg
     4201 ggatgccatc cttcggccct ccgcctcccg cttccggggc gggcgacgat ccgttttcgt
     4261 ttctgaacaa aggcggtggc ggttcttcag gcggcggagc gaccccgcct cccgcgggcc
     4321 cgttcggcaa tgccggcgcg gcgccaggat tcgtaccgtc caaacccgca ccgagcgcga
     4381 atccctatgc ctcgccggcc ggcgcggcat acagctatca gcccatggca cctggtccgg
     4441 gttatggcaa ccggccgggc ctaccctggg aagtcaaagg tcaaagtgtt tccacctggt
     4501 gggaaacggc caagctgtgc atgacccagc cgacctatgc cttcagcgtc atgcggcaat
     4561 acggcggcat gggccagccg atgctgtact gcggatacgg gctggccgcc ggcatgatcg
     4621 gtcaaatgct ttggtacttg ccgctgatgg gaatcatggg attgatgatg aatcaaggcg
     4681 gcaacggcca ggacgccgcc atgttcgtcg gcattcaagt ggtcagcagc atctttcaaa
     4741 gcgtcatcgg tgtcttgatc ggggcaacgc tgatgttgct cattggagca gccatcctgc
     4801 acgtctgcct gatgctcgtc ggcggggctc gccaaggctt tgaaaccacg ttgcgcgtgc
     4861 ttgggtacgc tcaaggttcc attgcttgga tgaacgtcat tccgatcttt gggcccctgg
     4921 tcggcgcaat ttggggcctg gtgatcgaaa tcattggcgt ggcccgcgcc cacgaaatcg
     4981 aaacggggaa ggccgtcctg gcggtatttc tgcccttgat cgcgtgctgc gtgctcgcgg
     5041 gcggcctgtt tgctttgatc atggcgttgg ggatcgccgg cgcaaacatg tgacgatctc
     5101 ggcggaggtc attcaatcgg caccgtgtag ccgagggcgc cgatcggttc gccgggcttg
     5161 gcgttttcgt agttcgggtg gcacattgtg catttcttga gcacgacggg aatcggcgtg
     5221 gcggcacgca ggtagcgctt gccgtccttt tcgatgacct ggtcgtaata gggcttgcca
     5281 cctttcaggg ccgcgatggc cgctttttcg aatgcgtcct tggcgacgtt cttgtcttcc
     5341 agcggctcgc cggtggcgtc gagcaggcgg acttcgtgat gccccttctt cttgattgcg
     5401 tcgaagaggg cgatcgccgc gctgccggcc ggcaggtcgt cggcgtcgtt gacgtagtgt
     5461 tcggtgatca gcacgaccgc ggccttatag acgtcgtcga gcacctgcac ctgcttccgc
     5521 gtgcgggcga ccgcggcgtc ttcttccttc ttgtcctcgg cctgcgagcc gctccagacg
     5581 aagaggctcg ccatcgccaa acccagcccc gcgcccgcca accaacgaga gaatcggacc
     5641 ttcatcgtgc tttctcctga tctgaatgaa acaaccggtg tgtgaaacga aaccgccaca
     5701 gccgcccgcg cgagaaggca gccctacttc cgaaccttgg accttggacg tttgactttg
     5761 gacctgcctc ccctgttccc tgttccctct tccttgtctc ctgcctgcct gatctctagc
     5821 agcgtcacgc cgggcatcag caaatcgctc agcttgacgc tctgcaaata ctccatctga
     5881 atccgctccg ccttcgccag aacggtcttc agccggcagc cgggttggat gacgcaaacg
     5941 ttttcgatgc cgacgcagtc gagcaggtgc atgttccctt cgcagtcgag aatgacctgc
     6001 ccgacgcgga tgtcctcggg ccttttggcc agctcaattc cgccgccgat cccgcgaatg
     6061 ctccgcacat agccctgccg caccagcgtc tgcaccacct tggcgatgtg gtccttggaa
     6121 atccgataga actcggccac ttccgccgca ctggcccgcc ccggattgct cgccagatac
     6181 agcagcgtcc gcagggcata gtcggtatgg agggcgagtc gcataagtgg ttaaacaggt
     6241 acttcttata cctgtttagg cgtaagtgta ggcggaatgc aaaggctgtc aagcggcagt
     6301 tgcgcgaccg acggaacgac acataactcg tttaaggcgc cccgccaccg atgcagtccg
     6361 cggcgctggg tgagaagggg ccgcttcggc gttcaaccga aaaggaacgc agggggagtg
     6421 gaccagacgt gtccctttcg ctgggcgatg ggaaaaaact tgcggtgcga gccgtgggcg
     6481 caagtccagg agtggctggc ggttggggcg aggcggattg gcgcaagtgg cgctggtgcg
     6541 gaattccggc cggaattcgt ttggagcgca gggaagcgat atgtaaactc gcgatcaata
     6601 gtgaacataa agtcggatcg gcgcgtcggg ttgggaccag gccgccgcgg ccgcaggccc
     6661 gccagcctgg gcgcggtggc gactgcgcga gctgcgccct cgggtcccgc cctgcgcggt
     6721 ccaaaccaga ccgcccatca tcgctgcgca gaaacggttg aaagccg
//
LOCUS       DASUGB010000499         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1306882, whole genome shotgun sequence.
ACCESSION   DASUGB010000499 DASUGB010000000
VERSION     DASUGB010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306882"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..526
                     /locus_tag="VFV87_09585"
     CDS             137..526
                     /locus_tag="VFV87_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104050.1"
                     /translation="MSASSEHALWEKIVDLAAGKAAAEAALGVHEDAMERTGELLEAL
                     AGVHAEKAKLEAQLETQAQLHELAAENVRLKAQVELAEAKAEVMGATVEMTVENERLK
                     MRLAELEGREGKAARTASRPRSEKKAR"
     gene            612..986
                     /locus_tag="VFV87_09590"
     CDS             612..986
                     /locus_tag="VFV87_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104051.1"
                     /translation="MRRPAVRFSLRWLLLASALIPAGLYWLALPTLHAQRYAAAINRG
                     DYAVADKLCVDPSRPFPGDWPRHKTFEPKASLKPVTWHDLRTGRRELFVGIAYGDASG
                     LASCGVEVSATRRGIEVGMFLP"
     gene            complement(1022..2227)
                     /locus_tag="VFV87_09595"
     CDS             complement(1022..2227)
                     /locus_tag="VFV87_09595"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5104052.1"
                     /translation="MDIHALTRELHTKNDSKIVMLVADGLGGLPMSPGGKTELETAVK
                     PNLDALAKVGIQGGSIPVCPGISPGSGPGHLGLFGYDPVQYLIGRGALEATGIGFAME
                     KGDVAARGNFCTLDSAGKITDRRAGRIPTEESAPLAIKLREVKIPGYQTFVEPVKEHR
                     FVVVFRGPGLAGAVKDTDPQATGVAPLPAEATDAPSGQMAEIANQWIAAATKLLAGEK
                     KANGLTLRGFAARPHLPTYEEVYGLKAAAIAVYPMYKGLARLVGMEIVGQAQTLAEQM
                     TVLEQNWSKYDFFFVHFKYTDSTGEDGNFAAKVKKIEELDAAIPRITALRPKVLIVTG
                     DHSTPSYLKSHSWHPVPTLLVSDCCRPDGHTSFGENMAIRGGLGQFKAKYLMTLALAN
                     AGRLAKYGA"
BASE COUNT          375 a          714 c          763 g          422 t
ORIGIN      
        1 cagcaactgc aaggcctgcc ccgctctcgc ctgccaggtg gcggccagcg gcgcatgcgc
       61 ctgtcccgga aagtgctgcc ccgacggcca gtgcgcgtgc gctgccaagg cttgtgccgg
      121 cggttgcccg agcagcatgt cggcatccag cgaacatgca ttgtgggaaa agatcgttga
      181 tttagccgcc ggcaaagcgg ccgctgaagc ggccttggga gtgcacgagg atgcgatgga
      241 gcgaaccggc gagctgctcg aagcgctggc cggcgtgcat gccgagaagg ccaagctcga
      301 agcgcagctg gaaacccagg cccagttgca cgaattggcg gctgaaaacg tccgcctcaa
      361 ggcccaagta gagctcgccg aggcgaaggc cgaggtcatg ggggccacgg tggaaatgac
      421 cgttgagaac gagcggctga agatgcggct ggccgagctg gagggacggg agggcaaggc
      481 tgctcgcacg gcctcccggc cgcggagcga gaaaaaggcc cggtaatgcc gagagccgga
      541 agatcctatt gccggatggg ctgaaaacca aacggcaggc ggcggcgtct cagctggaaa
      601 ggagacttgc catgcgccga cctgccgtcc gcttttcgct tcgctggctg ctcttggcca
      661 gcgccctcat tccggccggc ctgtactggc tggccctgcc gacgctccat gcgcagcggt
      721 acgccgcggc gatcaaccgc ggcgactacg cggtcgccga caaactctgc gtcgatccga
      781 gccgcccgtt tcccggcgac tggccgcggc acaagacgtt cgagcccaaa gcctcactca
      841 agccggtaac ctggcacgac ttgcgcaccg ggcggcgcga actgtttgtc ggcatcgcct
      901 atggcgacgc gagcggcctg gcgagctgtg gcgtggaggt ttccgccacc cgccgcggga
      961 ttgaagtcgg aatgtttttg ccctgatcga tcatcgcgga tcgccgcttt ccgaactcgt
     1021 cctatgcccc atacttcgcc agccgccccg cgttggccaa ggccagcgtc atgagatact
     1081 tggccttgaa ttgccccagg ccgccgcgga tcgccatgtt ttcgccgaag ctcgtgtggc
     1141 catcgggccg gcagcagtcg cttaccagca gcgtggggac agggtgccag ctgtggcttt
     1201 tgaggtagct gggcgtgctg tggtcgccgg tgacaatcag cactttcggc cgcaaggcgg
     1261 tgatgcgcgg gattgccgcg tcgagctcct cgattttctt caccttggcg gcgaaattgc
     1321 cgtcctcgcc ggtgctgtcc gtgtatttga agtggacgaa gaagaagtcg tacttgctcc
     1381 agttctgctc gagcacggtc atctgctcgg ccagcgtctg ggcctggccg acgatttcca
     1441 tgccgacgag ccgggccagt cccttgtaca tcggatagac ggcgatcgcg gccgccttta
     1501 ggccatacac ttcctcatag gtgggcaggt gcggccgggc cgcaaagccg cggagcgtca
     1561 ggccgttggc ctttttctcg cccgcaagca gcttcgtcgc cgctgcgatc cactggttgg
     1621 caatttcggc catctgaccg ctgggtgcat cggtcgcttc ggccggcaag ggtgcgacgc
     1681 cggtcgcctg cggatcggta tctttgaccg cacctgccag gcccggtccg cgaaacacca
     1741 caacgaagcg gtgctccttc accggctcga cgaacgtctg atagccggga atttttactt
     1801 cgcggagttt gatcgccagc ggggcgcttt cctcggtggg aatgcgcccg gcgcggcggt
     1861 cggtgatttt gcctgcgcta tcgagcgtgc agaagttgcc gcgggccgcc acgtccccct
     1921 tctccatggc aaagccgatg ccggtcgctt cgagggcccc gcgcccgatc aggtactgca
     1981 ccggatcgta gccgaataag ccgagatgcc ccgggccact gccgggactg atgcccggac
     2041 acacgggaat gcttccgccc tggataccga ctttggccag ggcgtcgagg ttcggcttca
     2101 cggccgtttc gagctcggtc ttgccccccg gcgacatggg caagccgccg aggccgtcgg
     2161 cgacgagcat cacgatcttg gagtcgtttt tggtgtgcag ctcgcgagtg agggcgtgga
     2221 tgtccatggg aagggttcag ggttcaggag ttagctttca gcatttgttg tgat
//
LOCUS       DASUGB010000500         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_891286, whole genome shotgun sequence.
ACCESSION   DASUGB010000500 DASUGB010000000
VERSION     DASUGB010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891286"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFV87_09600"
     CDS             <1..300
                     /locus_tag="VFV87_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HEX5104053.1"
                     /translation="GRPVDSRAMFPGGSEGSGLEGLRTYLREHRQQEFLDNFCRKLVS
                     YALGRSLQPSDDGLIEKMHQVGAAHGYRFGSLVECIVTSEQFLTKRGNVELTKDP"
     gene            303..1652
                     /locus_tag="VFV87_09605"
     CDS             303..1652
                     /locus_tag="VFV87_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5104054.1"
                     /translation="MTTARTGRETSGFSRRMLLRGAGVAMALPWLESVPVWGAETAAA
                     GSPDRKFPKRFAALFMACGINSKHWWAKGAGDQMELGRSLEPLAPLKTKVNYISGLFN
                     RAAVGVGIHPGQTGNILSGAALQKGSELRGGISMDQVLANSIGQLAAQPSLVLGCEQP
                     VTGYHETNFSMAYSSHISWQNSTSPVPMEVYPSLAFDSLFDNRGSRRNKSVLDRVRDE
                     AASLSRQVSSGDKAKLDEYLTSVREVEKRVDRARDDQSRAEASAEHRGQNVVTMPRPD
                     NGLPEDIREHMRLMCDIVALAFQTDKTRVATLLLCRDISGLFYPFLDARKAHHSASHD
                     DLSDEYERISRYYCSQLAYLATRLDAMPEGEGTVLDHSCLLFLSNMWSGSKHDSTKLP
                     LVMVGSLGGTLETGRVLDYLGKSDDDRKLCSLYLALMDRMGVTLPKFGDAETRLAGL"
     gene            1675..>2124
                     /locus_tag="VFV87_09610"
     CDS             1675..>2124
                     /locus_tag="VFV87_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5104055.1"
                     /translation="MRQSIAILVLLSCTWLAAPEAAFADPEGLKTLAIGDAAPDFKLP
                     GVDGKEYSLKSFADARLLLVVFTCNHCPTAQAYEERIIQLDADYKSKGVALVAISPND
                     PLAVRLDELGYTDLSDSFDEMKQRAKDRDYQFPYLYDGETQAASLAYG"
BASE COUNT          391 a          681 c          681 g          371 t
ORIGIN      
        1 ggcaggccgg tcgatagccg ggcgatgttt cccggcggca gcgaagggag cggcctggag
       61 gggctgcgga cctacctccg tgagcaccgc cagcaggaat ttctggacaa cttctgccgg
      121 aagctggtat cctatgccct cggacggtcg ctccagcctt cagacgatgg tttgatcgag
      181 aaaatgcacc aagtgggcgc cgcccacggc taccgatttg gcagcctggt ggaatgtatt
      241 gtcaccagcg agcagttttt gacgaagcgc ggcaacgtcg agttgacgaa ggacccctag
      301 agatgacaac cgcccgcaca ggccgcgaaa cctccggttt ttcccgccgc atgctcctcc
      361 gcggagcggg tgtggcgatg gctttgccgt ggctggaatc ggtgcccgtt tggggagcgg
      421 aaacggcggc tgccggttcg ccggatcgaa agttccccaa gcggtttgcc gcgctgttca
      481 tggcgtgcgg catcaactcc aagcactggt gggcgaaggg cgcgggcgac cagatggagc
      541 tgggccggag cctggagccc ctcgcgccgc tgaagacgaa ggtcaactac atcagcgggc
      601 ttttcaaccg cgcggcggtc ggcgtcggca tccatcccgg gcagacgggg aacatcctct
      661 ccggcgcggc cctacaaaag gggtcggagc tgcgcggcgg catcagcatg gaccaggtgc
      721 tggccaattc catcggccaa ctggcggccc agccgagtct cgtcctcggg tgcgagcagc
      781 cggtgaccgg ctatcacgag acgaacttct cgatggccta cagctcgcac atttcgtggc
      841 agaattcgac gtcgcccgtc ccgatggagg tttatccctc gctggcgttc gacagcctgt
      901 tcgacaaccg cggcagccgg cggaacaaga gcgtgctcga ccgcgtgcgg gacgaagcgg
      961 ccagcctcag ccggcaagtc agctcgggcg ataaggcgaa gctcgacgag tacctgacga
     1021 gcgtccgcga agtcgaaaag cgcgtggacc gcgcccgcga cgaccaaagc cgggccgagg
     1081 ccagcgccga gcaccgcggc cagaacgtcg tcacgatgcc gcggcccgac aacggcctgc
     1141 ccgaggacat ccgcgagcac atgcgactaa tgtgcgacat cgtcgcgctg gcgtttcaga
     1201 cggacaagac gcgcgtggcg acgctgctcc tttgccgcga tatttcggga ctgttctatc
     1261 cgttcctcga tgcccgcaag gcccaccatt cggcttcgca cgacgatctc tcggacgagt
     1321 acgagcgaat ttcgcggtac tactgcagcc agctcgccta cctggccacc cgcttggacg
     1381 cgatgccgga aggcgagggg accgtgctcg accactcgtg cctcttgttc ctctccaaca
     1441 tgtggtccgg cagcaagcac gacagcacca agctcccgct ggtgatggtc ggcagcctcg
     1501 gcggcacgct cgaaacgggc cgcgtgctcg actacctggg caagagcgac gacgaccgca
     1561 agctctgcag cctgtatctc gcgctcatgg accgcatggg tgtcacattg cccaaattcg
     1621 gcgacgccga aacccgcctg gcgggactgt aattgttctt attgagccgt tgtcgtgcga
     1681 caatcaattg ccattctcgt tctgctctcc tgcacctggc ttgcggcgcc tgaagcggcg
     1741 ttcgcggatc ccgagggctt gaagacgctg gcaatcggcg atgccgcgcc cgatttcaag
     1801 ctgcccggcg tggacggcaa ggagtattcg ctcaagagct ttgccgacgc ccggctgctg
     1861 ctggtcgttt ttacgtgtaa tcattgcccg acggcccagg cttacgaaga gcggatcatc
     1921 cagctcgacg cggattacaa atccaagggg gtcgccctgg tcgccatctc gccaaatgac
     1981 ccgctggccg tgcgcctgga cgagctcggc tacaccgatt tgagcgattc gttcgacgag
     2041 atgaagcagc gggccaagga ccgcgactac cagtttccgt atctctatga cggggaaacg
     2101 caggccgcct cgctggccta cggc
//
LOCUS       DASUGB010000501         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1306948, whole genome shotgun sequence.
ACCESSION   DASUGB010000501 DASUGB010000000
VERSION     DASUGB010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306948"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VFV87_09615"
     CDS             complement(<1..1332)
                     /locus_tag="VFV87_09615"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5104056.1"
                     /translation="MKPGLNGQESGVKGQESLLAPDSCLLTPLIENERYEFFAEPTYR
                     FNGDRRDFLRVVGGGIVICLLVPAEANAQQRGTGRRGFGGASGPQEIGGWLHIAEDGQ
                     VTVFTGKVEVGQNIRTSLTQAVADELRLPTGAIKLVMADTRLCPPDAGTFGSRTTPDM
                     AARLRRAASAAREMLLDLAAEQAQADRGTLDVSEGKIVNPATKVEYTYGELTKGQKIT
                     KTLAADVATTPVPKWQVGGTSVPKVGGRAIVTGKHQYASDVRLPGMLHGKVLRPPTLN
                     AKLVTVDLSAAKALPDVVTVRDGDFVGVAAPTAYKAEQALAAIKAEWSSQPEPSDKML
                     FEDLKRSGSGASAGVGRFGQGGDSRGSIERGLAAAGHQLQATYTIAYIAHAPLEPRAA
                     VAQWDGGQLTVWTGTQRPFGIQSDVARAFGLANEAMRVIVPDTGSAYGGKHT"
     gene            complement(1329..1796)
                     /locus_tag="VFV87_09620"
     CDS             complement(1329..1796)
                     /locus_tag="VFV87_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5104057.1"
                     /translation="MAISLLVNGEEREFAGDPARSLLSVLRDDLGLTGAKYGCGEGQC
                     GACTVLIDGKPIRSCITESGECAGRHIATIEGLEQAGRLHPLQEAFLACDAMQCGYCT
                     SGMIMSGAALLAEKPRPTREEIVRAMNGNICRCGTYQRIVAAIRQAAGEGGGR"
     gene            2032..3435
                     /locus_tag="VFV87_09625"
     CDS             2032..3435
                     /locus_tag="VFV87_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104058.1"
                     /translation="MRCSLVLICLCIFAGSFLARSATAQPPPTAALRAQIEELQRQLD
                     QAARRETQDSKVTEIGSSRGRSHVEEEPRLVVRIYDLSDLYSIAPQYPAREPADLQDA
                     SRAVFPETELSAASPSGFGGMGGGGFGGGFFAVPPGKISRGGEPATLHQVTSGTSGEH
                     LQSFRSAVESLIETITATISPDEWDAVGGPASITALGASLVVSAPADTHDKITALLDL
                     FRKRWGALRTVSLQAHWLWLTEDELAAAPAEFPWNDKATAAYGVMSDDAWKQLREAAA
                     ARAKERSGFHLALTCYNGQTVYALAGGQKLSVVGMTPVVGGSEKEAAYQPVVRVLQEG
                     AALQATPIATRTAKFVVVDVHSRVNLLDRAAGRPPAAGGEKAAKAGAVEQVIEALDRP
                     PLASQRLSTTLRIPTGRPTLVGGMTFSGAGGGEANLYLFLTARVQELRDEEPKVEQGG
                     EPDVPRENDTAIPAEKK"
     gene            3626..3913
                     /locus_tag="VFV87_09630"
     CDS             3626..3913
                     /locus_tag="VFV87_09630"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX5104059.1"
                     /translation="MPQNLAESPKLPPPSFALIVASFAAQASVALGHVPNPMTNKQEE
                     NLDLAKHAIDTLAILEQKTKGNLAPDEAAMLEDVLHQMRMAYLEAQKAGKA"
     gene            3917..>4104
                     /locus_tag="VFV87_09635"
     CDS             3917..>4104
                     /locus_tag="VFV87_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104060.1"
                     /translation="MRAIAAHVGSTEPRIVELAEPHQPGPGEVLCRTLELGICGTDRE
                     ILHSANPWMPEGDDFLVLG"
BASE COUNT          729 a         1333 c         1340 g          702 t
ORIGIN      
        1 ggtgtgcttg ccgccgtacg ccgagccggt gtccggtacg atcacgcgca tcgcctcgtt
       61 ggccaggcca aaggcccggg cgacatcgct ctgaatgccg aagggccgct gcgtaccggt
      121 ccaaacggtc agttggccac cgtcccactg ggccaccgca gcccgcggtt ccagcggcgc
      181 gtgggcaata taggcaatcg tgtaggtcgc ctgcagctga tgccccgctg cggccaagcc
      241 acgctcgatt gagccgcggc tgtcgccgcc ttgaccgaag cgcccaacgc ccgcggaagc
      301 gccgctgccg ctgcgtttca gatcctcgaa gagcatcttg tccgacggct cgggctggct
      361 ggaccattcg gctttgatcg cggccaaagc ctgctcggcc ttgtaagcgg tcggggcggc
      421 aacgcccaca aagtctccgt cgcgaacggt tacgacatcg ggaagcgcct tcgcagccga
      481 caaatcgaca gtcaccagct ttgcattcaa tgtcggcggg cgcagcacct tgccatgcag
      541 catgccgggc agccggacgt cggaggcata ctgatgcttg ccggtcacga tggcccggcc
      601 gccgaccttg gggactgacg tgcctccgac ttgccacttg gggacgggcg tcgtcgcaac
      661 atcggcggcg agtgttttcg taatcttctg cccttttgtg agctcgccgt aggtgtattc
      721 gaccttggtc gcggggttga cgatcttgcc ctcggagaca tcgagcgtgc ctcggtccgc
      781 ctgagcctgt tccgcggcca ggtcgaggag catctcccgc gcggccgacg cagcacgccg
      841 cagccgcgcc gccatatcgg gcgtcgtgcg gctgccgaat gtgccggcat cgggcgggca
      901 aagtcgcgta tcggccatca ccagcttgat agcgcccgtg ggcagtcgca gttcatcggc
      961 aacggcctgg gtgagcgatg tgcggatgtt ctgcccgact tcaaccttgc cggtaaagac
     1021 agtcacttga ccgtcctcgg cgatgtgcag ccaaccgccg atttcctgcg gtccgctcgc
     1081 tccgccaaat ccgcggcgac ctgtgccgcg ttgctgggca ttcgcctcgg caggcacgag
     1141 caggcagatg acgatgccgc cgcccacaac acggagaaaa tcgcggcggt ccccgttaaa
     1201 ccgataagtc ggctcggcga agaactcgta gcgctcgttc tcgatcaaag gagtcaggag
     1261 acaggagtca ggagccagca aagactcctg ccctttgact cctgattcct gaccattgag
     1321 tcctggcttc atcgtccacc tccctcgccg gcagcctggc gaatggcggc gacaattcgc
     1381 tgatacgtcc cgcaacggca gatgttgccg ttcatcgccc tgacgatttc ctcccgcgtg
     1441 ggccgcggct tttcggcgag cagcgccgcg ccggacatga tcattccgga tgtgcaatag
     1501 ccgcactgca tggcatcgca tgccaggaag gcctcctgca gcgggtgcag ccggccagcc
     1561 tgctccagtc cctcgatggt ggcaatgtgc ctacctgcac attcgccgct ttccgtgatg
     1621 catgaccgaa ttggcttgcc gtcgatcagt accgtgcagg cgccgcactg cccctcgccg
     1681 cagccgtatt tggcgccggt cagccccaga tcgtcgcgca agacgctgag gagcgagcgg
     1741 gccggatcac ccgcaaattc gcgctcctcg ccattaacca ggagagagat agccatcgat
     1801 tgcgtccccg gcataagtat gggcgcagcc tacttccgcc caggagcaaa cgccagccgg
     1861 ctggccggtc aaggcacggc cgtttggcgc gaccctgctg ccagaaacaa aatccacagc
     1921 gtaatacacc tcgagaattc gccgtgccga aactcccacg tggcggcggc tctgcgaatc
     1981 ggctaggctg ctggattcca ggggcaatcg ctagtgcaag aggaggctct catgcgctgc
     2041 tccctggttc tcatctgtct ctgcattttc gcaggctcat ttctcgcccg ttccgccact
     2101 gctcagccgc cgcccacggc ggcgctgcgt gcgcaaattg aagagctgca gcggcagctc
     2161 gaccaggccg cccgccggga aacgcaggat tccaaggtga cggaaatcgg ctcgtcgcgc
     2221 gggcgttcgc acgtcgagga ggagccgcgg ctggttgtac gcatctacga cctgagcgac
     2281 ttgtactcga tcgccccgca atacccggcc cgcgagcccg cggatctgca ggatgccagc
     2341 cgcgccgtgt tcccggaaac ggagctctcg gctgccagcc ccagtggctt cggcgggatg
     2401 ggaggcggcg gattcggcgg cggcttcttc gccgtgccgc cggggaagat cagccgcggt
     2461 ggcgagccag ccacgctgca ccaggtgacc agcggcacga gcggcgagca tctgcaatcg
     2521 tttcggtcgg cggtcgaaag cctgattgag accatcaccg cgacgatttc gccggacgag
     2581 tgggatgcag tcggcgggcc ggcctcgatc acggcgctcg gggcgtcgct cgtcgtctcg
     2641 gcgcccgccg atacgcacga caagatcacc gccttgctgg acctctttcg caagcgctgg
     2701 ggcgccctgc gcacggtgag cctgcaggct cattggctgt ggctgaccga agacgagctg
     2761 gccgcggctc ctgcggagtt tccgtggaac gacaaggcga ccgcggcgta tggcgtgatg
     2821 agcgacgacg cttggaagca gctccgcgaa gcggccgccg cacgggccaa ggagcgttcg
     2881 ggatttcacc tggcgctgac ctgctacaac ggccagaccg tttatgccct ggcgggcgga
     2941 cagaagctgt cggtcgtggg gatgacgccg gtcgtcggcg gcagcgagaa ggaagccgcc
     3001 tatcagccgg tcgtccgcgt gctgcaagag ggcgcggccc tgcaggcgac gccgatcgcc
     3061 acgcgaacgg ccaagtttgt cgtcgtcgat gtccacagcc gcgtgaatct gctcgaccga
     3121 gcggcaggcc gaccgcctgc cgcgggtggc gagaaggcgg ctaaggccgg cgcggtcgag
     3181 caggtcattg aggcgctaga ccggcctccg ctggccagcc aaaggctctc gacgaccttg
     3241 cgcattccga caggccggcc gacgctcgtg ggcggcatga ccttcagcgg cgcgggcggc
     3301 ggcgaggcga atttgtattt gttcctcacg gcgcgtgtcc aggaactgcg cgacgaggag
     3361 cccaaagtag agcaaggtgg cgagcctgat gtacctcggg aaaacgatac agcgataccg
     3421 gctgagaaga agtgaagtca ggaaacctgc cccgtattcc cacgtggggc aggtatcttg
     3481 tctgccataa agggatgtcg ccagccggtc gacgtcgcgt cgaaaaaacg tcgatcatcg
     3541 tacgggcctc gcgcaagaat cgtatcaaga tcggctggca gtctacattc ctcgatgcag
     3601 ggaaaccagt tatgctgacc agataatgcc tcagaatttg gccgaatcgc ctaaacttcc
     3661 gcccccctcc ttcgctctga tcgtggcctc attcgccgcg caggccagtg tggcgctcgg
     3721 tcacgtgccg aacccgatga cgaacaagca ggaagaaaac ctcgacctgg ccaagcacgc
     3781 gatcgacacg ctggcgatcc tggagcagaa gacgaagggg aacctggctc cggacgaggc
     3841 ggcgatgctc gaagacgtgc tgcaccagat gcggatggcg tatttggaag cccagaaggc
     3901 gggaaaggcg taagccatgc gcgccatcgc cgcccacgtc ggctcgaccg agccgcggat
     3961 tgtcgagttg gccgagccgc accagccggg gccgggcgaa gtgctgtgcc gcacgctgga
     4021 actggggatt tgcggcaccg accgcgagat cctgcattcg gccaatccgt ggatgccgga
     4081 gggggatgac tttctggtgc tggg
//
LOCUS       DASUGB010000502         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1445176, whole genome shotgun sequence.
ACCESSION   DASUGB010000502 DASUGB010000000
VERSION     DASUGB010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445176"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFV87_09640"
     CDS             complement(<1..633)
                     /locus_tag="VFV87_09640"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5104061.1"
                     /translation="MRRSWLAIAGRLALRVLRLGAVVYLAVLLMLLLLENTLLYPAPK
                     YPEGDWDAAYLPHEDVHFASADGTRLHGWLVEHPQPRAVVLYLHGNGDCVGFLGPYLQ
                     QLSDNHQVTIFAFDYRGYGRSEGSPGEQGILEDGHAAQMWLAERLQIEPADIVLMGRS
                     LGGGVAVDLATKNGARGLILQNTFTSLPDAAACMYPWAPVQWLMKNRYDSL"
     gene            complement(793..1686)
                     /locus_tag="VFV87_09645"
     CDS             complement(793..1686)
                     /locus_tag="VFV87_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HEX5104062.1"
                     /translation="MIHNPLARRDFLRTLAAGGVCLAAPALAQEGESLREPVFRVSKT
                     NNEPQEGPAGHPLDPAIKLAQESLAHFQNTVVDYTALIVSRERVNGVVGQYEYMEAKI
                     RNQKIENGRVVKPLSVYLKFVKPQAAAGREILWLQDENNNKMLVNPSDGDGRFIPSIW
                     MKPDGPIAMRGRLHPLTFIGIENLIVKLIERGERERRYGECDVRFIKGAKINDRVCTV
                     LEVEHPVPRQHFEFNVAQIFIDDELQMPIRYAAYLWPTTASEKRLDPGLVIEEYTYLK
                     VKLNQDLTDADFSRNNPNYQF"
BASE COUNT          418 a          703 c          686 g          414 t
ORIGIN      
        1 cagcgaatcg tagcggttct tcatcagcca ctggaccggg gcccagggat acatgcaggc
       61 agccgcgtcg gggagggacg tgaacgtgtt ttgcaggatc aggccgcggg cgccgttctt
      121 ggtcgccaga tcgaccgcga cgccgccccc cagcgagcgg cccatcagca cgatatccgc
      181 cggctcaatc tgcagtcgct cggccagcca catttgggcc gcgtggccgt cttcgaggat
      241 accctgctcg cccggcgagc cttcgctcct gccgtagccg cgataatcga aggcgaagat
      301 cgtgacctgg tggttgtcgc tgagctgctg caggtacgga cccaggaagc cgacgcagtc
      361 gccgttgccg tgcagataga gcacgacggc ccgcggctgc ggatgctcga ccagccagcc
      421 gtgcagcctc gtgccgtcag ccgaggcgaa gtgtacatcc tcatgcggca ggtaagccgc
      481 gtcccagtcc ccctcgggat acttgggggc aggatagagg agcgtatttt ccagcaagag
      541 gagcatgagc aggactgcca aatagacaac ggcgcccagc cgcagcacac gcagcgccaa
      601 gcgaccggcg atcgcaagcc aggagcgtct cacacgcgga attgtaccgg ggcggggcgg
      661 cggaggcacg cgcatgccaa tcgcccacct catgcaggcg ccgcagagcg gcaagcgcgg
      721 gcgttcccac gcggagcgtg ggaacgagac ttgaggaaga tacgtttgga cgggttggaa
      781 acccgtccta ctctagaact gatagttcgg attgttacgc gagaaatctg cgtcggtgag
      841 atcctgattc agcttcactt tcaggtacgt gtattcctca atcaccaaac ccgggtcaag
      901 ccgcttctcg ctcgcggtcg tgggccagag gtaagccgca tagcgaatcg gcatctgcag
      961 ctcgtcgtcg ataaagatct gcgcgacatt gaactcaaag tgctgccgtg ggacaggatg
     1021 ctcgacctcc agtaccgtgc agacccggtc gttgatcttg gcccctttga tgaaccgcac
     1081 gtcgcactcg ccgtaccgcc gctcgcgctc gccgcgctcg atcagcttga cgatcaggtt
     1141 ctcgatgccg atgaacgtca gcgggtgcag gcggccgcgc atcgcgatcg gcccatcggg
     1201 cttcatccag atcgacggaa tgaaccggcc gtcgccgtcg ctcggattga cgagcatctt
     1261 gttgttgttc tcgtcttgaa gccacaagat ctcgcgcccc gccgccgcct gcggcttgac
     1321 gaacttgaga tagaccgaca gcggcttgac gacgcggccg ttctcgatct tctggttgcg
     1381 gattttggcc tccatgtatt cgtactgccc gacgacgcca tttacgcgct cgcgggaaac
     1441 gatcagcgcc gtgtaatcga ccaccgtgtt ttgaaagtgg gccagcgatt cctgggccag
     1501 cttgatcgcc ggatcgagcg gatgacctgc cggaccctcc tgcggctcgt tgttggtctt
     1561 cgagacccga aacaccggct cgcggagcga ttcgccttcc tgggcgagcg cgggggccgc
     1621 caggcagacg ccgccggcgg cgagggtgcg gagaaaatcg cggcgggcga gggggttatg
     1681 aatcacctgc ggggtctcct ttcagcgtcc gctctctgcc cgcgttggcc tttcggctgg
     1741 ggcagcggtc ggttttcgtc cttgaaatat ccgcgccggc gattgccagc aaatgttgca
     1801 ccggcaagtc ggctgctcgg atgcgcggca acttgcaggt ctcgggaatc gtcctaaatt
     1861 attgctgcga aatgacttgc aattcgtcac gcgccgacaa tccggaggtc caactccacc
     1921 gattgtcttg ggtcttatcg ccacgctgac tgggcacaat tacaaaaacc ctgcccccgc
     1981 accttcgcta gcctcactgg cgagctttgc gccagcatca cgactcgacc atcccgcacc
     2041 atcctaagcg gcaaacggca ggcgagggga gagcaaaatc gctgtgcaaa cccgccactg
     2101 cagtcgctcc ttcgtttaac gcgcagccga aggcgttgcg gtcttccggg tggaggccgc
     2161 attcggctac gcctgcggct gcgctttaaa cgacacgcca aacgaacgac ttacaaaatc
     2221 c
//
LOCUS       DASUGB010000503         4684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1168653, whole genome shotgun sequence.
ACCESSION   DASUGB010000503 DASUGB010000000
VERSION     DASUGB010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168653"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2244)
                     /locus_tag="VFV87_09650"
     CDS             complement(<1..2244)
                     /locus_tag="VFV87_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HEX5104063.1"
                     /translation="MNEPTLPEESLFLQALELDSAADQAAFLDRQCGSNAELRSSVEG
                     LLRHHREGSRFLESPPTEVIVAGEQLTAGQAETPALDFLTPSAEPASIGRLGHYEILE
                     LLGSGGFGVVLKARDAKLDRIVAVKTLAQSLASSATARRRFVREAKAAAAVRHENVVG
                     IYHVSDEGPVPYLVMECVSGLSLEQKLQQAGMLDVAAILRIGMQVASGLAAAHKQGLV
                     HRDVKPGNILLENGVERVKITDFGLARAANDAAITRTGEVAGTPQYMSPEQALGQPVD
                     ARSDLFSLGSVLYAMCTGRSPFRAETTIAALRRVCDDAPRPIREVNSDIPAWLVAIID
                     RLLAKKPDDRFQTAAEVADVLGQHLAHLQHPSAVGQASRLPVVTTAGQRPAPRARPWA
                     IAAAALVLLLLGISLTEATGVTNVVPTVIRIVTGEGTLIVETDPDVQVTLESNGVLNF
                     RVAGGQSIRVPTGEYRVKATKDGQQVPLEKDLVTISRGGEQIVRVRLERGPGTIAGKE
                     PPPSAIVPFDAATAKEHQEAWAKYVGLPVEFANSIGMKFRLIPPGEFEMGSTPQDIEA
                     ARAAEKYSDDQAVSDALTSEGPIHRVVLSEPFYLSVTEVTQQDFETVTGENPSTFRLG
                     GENAEKVKDADAGQLPAQGTSWNDAANFCSRLSVREGLISNYRPVGTAVEMLPGNGYR
                     LPREAQWEFACRAGTTTRFWFGNDPADHANREWLQANSAGRPQRVGSLAKNPFGLFDM
                     HGNVSEWV"
     gene            complement(2410..3045)
                     /locus_tag="VFV87_09655"
     CDS             complement(2410..3045)
                     /locus_tag="VFV87_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104064.1"
                     /translation="MTDVTQILSQIEQGDPSAAEQLLPLVYDELRKLAAAKLAQEKPG
                     QTLQATALVHEAYLRLVGPAGSAGLEEERHTPNQSRRAGGTYDSRGHFFAAAAEAMRR
                     ILVENARRKQRERHGGKLQRVEFPEEGVPDSPRDEQLILLDEALNRLLEIRPQAARIV
                     QLRYFGGQTVEEASTIVGISERTARRLWVFAQAWLRREMEALDEGKSLADK"
     gene            complement(3401..>4684)
                     /locus_tag="VFV87_09660"
     CDS             complement(3401..>4684)
                     /locus_tag="VFV87_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104065.1"
                     /translation="SLLAPDWDKELSPYDFQPNDTKFYGSVGFVAAGMLSDGGSVIQV
                     PLRGSGGTGLNNGAGTDLVGFSSSGRRRWVHKLAEHKGIAGLGTVDDITLTSVFYNCE
                     TIAVDADGLALGGFCEPQQLRYVGYWIDHPNLRLFKLPDGQTYATWGDNADGRHPWYR
                     LENQNSLIRSKYPFRLGEDRARQLAAREAKPAAGAKNIAQPEVRIPRLEKPLPIDGEL
                     EKWRKAGIKPVCALGPLGSFKGPRDCSAVIRMAYEGQNLYFQVLEFDDVPIFYAMVYE
                     DCVELAINGACGPGFQFICYKDAAGKDHIWRNRFFMANSQKTLEPQHAPRIVKVLPDA
                     EAVPERQLLENLYGVDLSKSKVVVSEFKLPIDNVSFSGAEQDIFQLGPGKSFTIGFFI
                     DDNDTPYTDIQRFLQWPAAFGMFNPPEDGARVTCD"
BASE COUNT          844 a         1482 c         1464 g          894 t
ORIGIN      
        1 gacccattcg ctgacgttgc cgtgcatatc gaatagcccg aacgggtttt tcgcgagcga
       61 tccgacgcgc tgaggtcgtc ctgcggagtt cgcctggagc cactcccggt tggcatggtc
      121 ggcgggatca ttgccaaacc agaagcgcgt ggttgttccc gcgcgacagg cgaattccca
      181 ctgcgcctct cgcggcaaac ggtagccatt ccccggaagc atctcgaccg cagtaccgac
      241 cggccggtag ttcgagatga gcccctcacg cacactcaac cgcgaacaga aattggcggc
      301 gtcgttccag ctagtcccct gcgcagggag ttgcccggcg tctgcgtcct tcactttttc
      361 agcgttttcc ccgcccagcc ggaaggtgga cgggttttct cctgttacgg tctcgaagtc
      421 ctgctgcgtg acctcggtga cactgagata gaagggttcc gaaaggacga cgcgatggat
      481 cggcccttcg ctggtgagtg cgtccgacac ggcctggtca tccgagtact tttcagccgc
      541 ccgagccgct tcgatgtcct ggggagtgct gcccatctcg aattcgcccg gcggaatgag
      601 gcggaacttc atgccgatgg aattggcgaa ctcgaccggc aaaccgacgt acttcgccca
      661 ggcctcctgg tgctctttcg ccgtggctgc gtcgaacggg acgatcgcgg acggcggggg
      721 ctccttgccg gcgatcgtgc caggcccgcg ctctagcctc acgcggacaa tctgctcgcc
      781 gccgcgcgaa atggtgacca ggtccttttc caggggaacc tgctggccgt ccttggtggc
      841 tttgactcga tattcacccg tgggcacgcg aatcgattgc ccgccggcga cccggaagtt
      901 gagaacgccg ttgctttcga gggtgacctg cacgtcgggg tcggtctcga cgatcagtgt
      961 cccttcgccg gtgacgatgc ggatcaccgt cggcacgacg ttggttacgc cagtggcttc
     1021 ggtcagcgag ataccgagga gcagaaggac cagcgcggcg gcggcgatgg cccaggggcg
     1081 cgctcgtggg gcgggcctct ggcctgccgt ggtgacgact ggcaggcgag acgcctgccc
     1141 cacggcggaa ggatgctgca ggtgcgccag atgctggccg agaacatcgg cgacttcggc
     1201 agcggtctgg aagcggtcgt cgggcttctt cgccaacagc cggtcgatga tcgcgacgag
     1261 ccaagcggga atatccgagt tgacctcgcg aatcggccgg ggcgcgtcgt cgcagacgcg
     1321 gcggagggcg gcgatggtcg tttcggcccg gaacggcgag cggccggtac acatcgcata
     1381 cagcacgctc ccgaggctga agaggtcgct gcgggcatcg accggctggc ccaaagcttg
     1441 ctcgggcgac atgtactgcg gcgtgccggc gacctcgcca gtgcgcgtga tcgccgcatc
     1501 gttggcggcg cgggccaggc cgaaatcggt gatcttcacc cgctcgacgc cgttttccag
     1561 cagaatgttg ccgggcttga catcgcgatg cacgaggccc tgcttgtgag cagccgccag
     1621 gccgctggcg acctgcatgc cgatccgcag gatcgcggcc acgtcgagca tgccggcctg
     1681 ctggagtttt tgctccagcg agaggccgct gacgcactcc atcacgagat acgggaccgg
     1741 cccctcgtcc gagacgtgat agatgccgac gacattctcg tgcctgacgg ctgcggcggc
     1801 cttggcttcg cggacgaatc gccggcgggc ggtggccgag gacgcgagcg actgggcgag
     1861 cgttttgaca gcaacgatgc ggtccaattt ggcatcgcga gccttgagca ccacgccaaa
     1921 cccgccgctg ccaaggagtt ccagaatctc gtagtgtccc aggcgcccaa tcgacgcggg
     1981 ctcagccgaa ggcgtcagga aatcgagggc gggagtctcc gcctgtccag cagtgagctg
     2041 ctcgcctgcc acgatgacct ccgtcggcgg cgattcgaga aaacggctcc cctcccggtg
     2101 atgacggagt agtccctcca cgctgctgcg cagctcggcg ttgctgccgc actgccggtc
     2161 gaggaaggcg gcttgatcgg cggccgaatc gagttcgagc gcctgcaaaa agagggactc
     2221 ttcgggcagt gtcggctcat tcatgggatc acgtgatgag ataatcgcct gcggcgcggc
     2281 ctcatttcgt cgcctttcgc tccgcgaaaa ggcgttcttt cgcggagcga aagacgacaa
     2341 tcagtgcctc acagctcaat gcgacaagcc gctgcaaaag cggccaagag aatttacaga
     2401 atgctgcaac tatttatccg ccaaggactt gccctcgtcc agcgcctcca tctcccgccg
     2461 cagccaggcc tgggcaaaga cccacagccg gcgggccgtc cgctccgaaa tcccgacgat
     2521 tgtcgacgct tcctcgactg tctgaccgcc aaagtagcgg agttgcacga ttctcgccgc
     2581 ctgcgggcgg atttccagta accgattgag cgcttcgtcg agcaaaatca attgctcgtc
     2641 acgcggagaa tccgggactc cttcctctgg aaactccacg cgctgcagct tgccgccgtg
     2701 cctttcgcgc tgcttgcggc gggcattctc caccagaatc cgccgcatcg cctcggccgc
     2761 ggcggcaaag aagtggccgc gggagtcgta ggtcccgccg gcccggcggg actgatttgg
     2821 cgtatggcgt tcctcttcaa gtcccgcgga gcccgcagga cctaccaagc gcaaatacgc
     2881 ctcatgcacc agcgccgtcg cttgcaacgt ctgccccggt ttctcttgag ccagcttggc
     2941 agcggcaagc ttgcgcagct cgtcatatac cagcggcaac agttgctcag cggcactggg
     3001 atcgccttgc tcaatctgcg agagaatctg cgtaacgtcg gtcatttcga ccaggatact
     3061 gccgaggaaa ggaggttgcc attgatctgg gcttccagcg gcaaacgttc gcagaggtga
     3121 accagaccgc ctgcagcttc tgcgaatgca aactcgcagc ggttgcatcg cccgccggga
     3181 attcagccag tcgccagcag cgcttttcgc cccaattcag gttaatctgg gtgcgggccc
     3241 atctgtgcaa cctgcgtggg ttgcgccttc gtcacacgag cggcgggagg ccatgcaaga
     3301 ggccgttcca aaggctgtcg gctgacggag ccgggtacgg ttctatgacc gacgcgctaa
     3361 aactttgctg cgaccacagt ggaggttaac ggtaacggga tcaatcgcaa gtcacacgcg
     3421 ctccgtcctc cggcgggtta aacattccaa acgcggcggg ccattgcagg aatcgctgta
     3481 tgtcggtgta gggcgtgtcg ttgtcgtcga tgaaaaagcc gatcgtaaag gacttgcctg
     3541 gaccgagttg aaaaatatcc tgctcggctc ctgaaaacga gacgttgtcg atcggcaact
     3601 tgaactccga aacaacgact ttggatttgg acaggtctac gccgtaaagg ttttccagaa
     3661 gctgtcgctc cggaaccgct tccgcatcgg gcaggacctt tacgattcgt ggcgcgtgtt
     3721 gcggttcgag agttttctgc gagttcgcca tgaagaagcg gttgcgccaa atgtgatcct
     3781 tgcccgcggc atccttgtag cagatgaact gaaaacctgg accgcaggcg ccgtttatcg
     3841 ccaattcaac gcagtcttca tacaccatcg catagaatat cgggacgtcg tcgaactcca
     3901 ggacctggaa gtagagattt tgcccttcat acgccatgcg gatcaccgcg ctgcaatcgc
     3961 gcggcccctt gaatgaacca agcggcccga gcgcgcagac gggcttgatc cccgctttgc
     4021 gccacttttc gagttcgccg tcgattggca gcggcttctc cagccgagga atcctgactt
     4081 ccggctgcgc gatgttcttc gctccggccg ccggcttcgc ctcccgcgct gcaagctggc
     4141 gagcgcggtc ttctcccagg cggaaggggt acttgctgcg tatcaggctg ttctggttct
     4201 ccagccggta ccagggatgc cggccatcgg cgttgtcgcc ccaggttgca tacgtctgcc
     4261 cgtccggcaa cttgaacagc cgcaggttgg gatggtcgat ccaatagccg acgtacctca
     4321 gttgctgcgg ttcgcaaaac ccgccgaggg caagaccatc ggcgtcgacg gcgatcgtct
     4381 cgcagttgta aaacaccgac gtgagcgtga tgtcatcgac cgtccccagg cccgcgatgc
     4441 ccttgtgctc ggcgagcttg tgcacccagc gccggcgacc gctcgatgag aatcccacca
     4501 ggtctgtgcc cgcaccgttg ttcagtcctg tgccgccgga gccgcggagc gggacctgga
     4561 tgaccgaacc gccgtcggac aacatccccg ccgccacgaa tcccacgctg ccatagaact
     4621 ttgtgtcgtt cggctgaaaa tcgtacggcg aaagttcctt gtcccagtcg ggcgcgagca
     4681 ggct
//
LOCUS       DASUGB010000504         5968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_335613, whole genome shotgun sequence.
ACCESSION   DASUGB010000504 DASUGB010000000
VERSION     DASUGB010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335613"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1483)
                     /locus_tag="VFV87_09665"
     CDS             complement(113..1483)
                     /locus_tag="VFV87_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104066.1"
                     /translation="MLTILGKSSSRFCDRASRRDFLTIGGMALGGLALPQILRAEADT
                     NSTRGRGHKAIINVYLPGGPPHIDMWDLKPDAPAEIRGEYSPIKTNVPGMEICELFPK
                     LASMAERFAIIRSLADSDGDHAAWQCMTGRGRGEQPPPGGWPAAGAWVSHVKGSVNPS
                     VPAHVSLMYKTGERRWGDPADGGFLGMGHAPFRLVGGKDNGMKSDNMVLQGITLDRLS
                     DRDSLRASLDQFRRAADASGSMDGLDEFSQKALGILTSSKLADALDLSQEDPKSLEKY
                     GVDDPAFERDGAPRMVRNFCIARRLVEAGARCVSMNFIRWDWHGGDGMNFVNSKRDFP
                     LLDSGLSSLLTDLHERGMDRDVSVVVWGEFGRTPRLNNRNSRDHWPQANACVLAGGGM
                     RTGQVIGATNRYAEHPTDRPIKFQEVFATLYTKAGLSLNTRQFDLRGRPQYLVDPGVE
                     PIRELV"
     gene            1679..4228
                     /locus_tag="VFV87_09670"
     CDS             1679..4228
                     /locus_tag="VFV87_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5104067.1"
                     /translation="MKLRELVLVCGIVFGLAVVLRGEEADREGIEFFEKKIRPVLVEH
                     CYECHSAGAKQVEGALLLDSKTGALKGGEQGPAVVPGDLDKSLLLRAIRYVDKDVQMP
                     PAPKERLPAEVIADFEAWIKRGAPDPRDEPAVTSAYHVDIEERKGKWPFTAPVDPPIP
                     AVKQADWPRNPIDHFILAKLEEKGIAPLADADKRTLIRRVTIDLTGLPPTVEEVDRLL
                     SPSPTRPLSPSGPSEGERGRVGEWEKRYAGYVEQLLASPHYGERQGRHWLDVVRYADT
                     AGDNSDYPIPQMYRYRNWVINAFNADMPYDEFIRQQIAGDLLPADSVAASHEQIVATG
                     YIALARRFGSTVDDYPTHLTIEDTIDNLGRTFLGLTINCARCHNHKFDPVTNEDYYAL
                     YGIFHSTRFPWPGIELDKVQRDLVPLTSDEEVAAAKEARKVKLDELTAAVKAVEDEKK
                     SIQKEENGEEKSKRLDEVEKKLRSAKKQREDFEKEPLPYEQAYAVAEGKTIGNCQVQL
                     KGDPTRLGTEVPRRFLQVLGGQALPVEEKGSGRVELANWIASQNNPLTARVMVNRLWQ
                     QHFGRGLVATPNDFGKQGQPPTHPELLDWLAMRFIESGWSVKEMQRLMVMSRAYQLAS
                     QESGVRGQESVAGGQEPVASADNPKSKIQNPKLLDPANSLLWHHNRRRLDAESIRDTL
                     LMLGGNLDPTMGEGHPFPPQKDWDFTQHKPFKAVYDTNRRSVYLMTQRIQRHPLMATF
                     DGPDTGASTGSRITSTTTLQALYFLNDPLVHAQANRIADRVRNSADNDAGRIDHAYNL
                     LYSRPATEDEQSLAAGYLSKLRSQAGDNPEAAAWQSLIRVLLRTNEYVYVE"
     gene            4754..5278
                     /locus_tag="VFV87_09675"
     CDS             4754..5278
                     /locus_tag="VFV87_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104068.1"
                     /translation="MNRIVFIAVSSALCLGSSGTLVAETKVTISKTHLCCPQCLTGVA
                     AALKDVDGVTHKCDQKAKTIELAAESDAAAQKAVDALAAAGFYGATDSDTVKYKAVEA
                     PEGELERLEVAGIHNCCGACTVAIKKAIDTVDGTTANTVKAKESKFVVEGKFSAEKLV
                     QALLDAGFYVQVKK"
     gene            complement(5280..>5968)
                     /locus_tag="VFV87_09680"
     CDS             complement(5280..>5968)
                     /locus_tag="VFV87_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104069.1"
                     /translation="SIRSLDQFKLETGGDANRRAAIQSAAAAPRAAENDLLKFVQTRT
                     TSALEVSRRIESSGQSYKTSVAYPGTPLAEKLKRIAQLIDAGLATRVYYVALDGFDTH
                     SDQAAAHSGLLQQLGGALAAFAEDLQAHGQLDRVTTLVFSEFGRRVNENASRGTDHGA
                     AAPVFLVGDRVKSGLIGKHPSFADLEDGDLKFHTDFRAVYAALLQNWLGWPSSPILGD
                     DFKPADVLTT"
BASE COUNT         1120 a         1790 c         2041 g         1017 t
ORIGIN      
        1 cgcggtggaa tggtcggcgc gcctccgcgc catgctcgcg agcgcggcga cgctcgccct
       61 gacactcggc tcactcacac tcatccaacg cgtcggcggt tgatagcggt ccctacacca
      121 actcccgaat cggctccacg cctggatcga cgaggtattg cgggcggccg cggaggtcga
      181 attggcgggt gttgagggag aggccggctt tagtgtagag ggtggcgaag acttcttgga
      241 acttgatggg gcggtcggtg gggtgctcgg cgtagcggtt ggtggcgccg atcacctggc
      301 cggtgcgcat cccgccgccg gcgaggacgc aggcattggc ttgcggccag tggtcgcggc
      361 tgttgcggtt gttcagccgc ggcgtgcggc cgaactcgcc ccagacgacg accgagacgt
      421 cgcggtccat tccgcgctcg tgcaggtcgg tgagcagcga tgacaaaccg gaatccagca
      481 aggggaagtc ccgcttgctg ttgacgaagt tcatcccgtc gccgccgtgc cagtcccagc
      541 ggatgaagtt catcgagacg cagcgggccc cggcctcgac caggcggcgg gcgatgcaga
      601 agttgcggac catccgcggc gcgccgtcgc gctcgaacgc ggggtcatcg acgccgtatt
      661 tctcgagcga cttcgggtct tcctgcgaca ggtcgagggc gtcggccagc ttcgatgacg
      721 tcaggatgcc cagcgccttc tgcgagaatt cgtcgaggcc gtccatcgag ccgctggcgt
      781 cggccgcgcg gcggaactgg tcgaggctgg cccgcagcga atcgcgatcc gagaggcgat
      841 cgagcgtgat cccctgcagg accatgttgt ccgacttcat gccattgtct ttgccgccga
      901 cgaggcggaa cggggcatgc cccatcccca ggaagccgcc gtcggccgga tcgccccacc
      961 gccgctcgcc cgtcttgtac atcaggctga cgtgagcggg caccgacggg ttgacgctcc
     1021 ccttcacgtg gctgacccac gcgcccgccg ccggccagcc gccgggcggg ggctgctcgc
     1081 cgcggccgcg gccggtcatg cactgccagg cggcgtggtc gccgtcgcta tcggccagcg
     1141 aacggatgat cgcgaaccgc tcggccatgc tggcgagctt gggaaacagc tcgcagattt
     1201 ccatcccggg cacattcgtc ttgatcgggc tgtattcgcc gcggatctcc gccggggcgt
     1261 cgggcttgag gtcccacatg tcgatgtggg gcggcccgcc ggggagatag acgttgatga
     1321 tggctttgtg gcctcgcccg cgcgtcgaat tggtgtccgc ctccgcgcgg agaatctgcg
     1381 gcagggcgag gccgcccaag gccatgccgc cgattgtgag gaagtcgcgg cggctggcgc
     1441 gatcgcagaa gcggctggac gatttgccga ggatggtgag catggggggc tccaggcggg
     1501 aatgtcgtcg ttcgctccgc gaacgagcgc cctttcgcgg agcgaaaggc gacaataatc
     1561 ttcccaaact gccggggtag attcaataca tatccaagaa tgtttatcgg agaaacgggc
     1621 ggaaggactt gcggccattg ggcgttggat acactaaagc ttctgcgagg gcgctgggat
     1681 gaagctgcgc gaattggtgc tggtgtgtgg gatcgtattt ggcctggcag tcgtgctgcg
     1741 cggcgaggaa gccgaccgcg aggggatcga attcttcgag aagaagattc ggccggtgct
     1801 cgttgagcat tgctacgagt gccacagcgc cggtgcgaag caggtcgaag gcgcgctgct
     1861 gctcgattcc aagacgggcg cgctcaaggg gggcgaacag ggtccggctg tcgtgccggg
     1921 cgatttggac aagagcctgc tgctgcgggc gattcggtac gtcgacaaag acgtgcagat
     1981 gccgccggcg ccgaaagagc ggctcccggc cgaggtgatc gccgatttcg aggcctggat
     2041 caagcgcggc gcgcccgacc cgcgcgacga gccggcagtc acaagcgcgt atcacgtcga
     2101 tatcgaagaa cgaaagggca agtggccgtt caccgcgccg gtcgatccgc cgattcctgc
     2161 cgtcaagcaa gccgattggc cgcggaatcc gatcgaccat ttcatcctgg cgaagctcga
     2221 agagaaaggc atcgcgccgc tggccgacgc cgacaagcgc acgctgattc gccgcgtgac
     2281 gattgacctg acgggattgc cgccgacggt ggaggaggtg gaccgcctgc tctccccctc
     2341 tcccactcgc cccctctccc cgtctggacc cagcgagggg gagaggggga gagtgggaga
     2401 gtgggagaag cggtatgcag gctatgtcga gcagcttctt gcttcgcccc actatggcga
     2461 gcggcagggg cggcattggc tggatgtcgt gcgctatgcc gacacggcgg gcgacaattc
     2521 ggactaccca attccgcaga tgtaccgcta ccggaactgg gtcatcaacg ccttcaacgc
     2581 ggacatgccc tacgacgaat tcatccgcca gcagatcgcg ggggatttgc tgccggcgga
     2641 ttcggtggcg gccagccacg agcagatcgt ggcgacgggc tatatcgcgc tggcccggcg
     2701 gtttggctcg accgtggacg attatccaac ccacctgacg attgaagata cgatcgacaa
     2761 cctggggcgg acgttcctgg ggctgacgat caattgcgcc cgctgccaca accacaagtt
     2821 cgatccggtc acgaacgagg actattacgc cctgtacggc atcttccaca gcacgcggtt
     2881 tccgtggccg gggattgagc tcgacaaggt gcagcgcgac ctggtgccgc tgacttcgga
     2941 cgaagaggtc gctgccgcga aggaggcccg gaaggtgaag ctcgacgagt tgaccgccgc
     3001 ggtgaaggct gtcgaggacg agaaaaagtc aatccagaaa gaggagaatg gcgaggagaa
     3061 gtcgaagcgg ctggacgagg ttgagaagaa gctgcggtct gcaaagaagc agcgcgagga
     3121 ctttgagaaa gagccgctgc cgtacgagca ggcctacgcc gtcgccgagg gcaaaacgat
     3181 cggcaactgc caggtgcagc tcaaagggga cccgacgcgg ctggggacgg aagtgccgcg
     3241 gcggtttttg caggtcctgg gcggacaggc attgccggtt gaagaaaaag ggagcgggcg
     3301 cgttgaattg gccaattgga tcgccagcca gaacaacccg ctgacggctc gcgtcatggt
     3361 caaccgcctc tggcagcagc actttggccg cggcctggtc gccacgccca acgacttcgg
     3421 caagcaaggc cagccgccga cgcaccccga gctgctcgac tggctggcaa tgcggttcat
     3481 cgagagcggc tggtcggtga aggagatgca gcggctgatg gtgatgtcga gggcgtacca
     3541 gctcgcaagt caggagtcag gagtcagggg gcaggagtca gtagctggtg gccaggagcc
     3601 agtggccagt gccgacaatc caaagtccaa aatccaaaat ccaaaattgc tcgaccccgc
     3661 caactcgctc ctctggcatc acaaccgccg ccgactcgat gcggagtcga tccgcgacac
     3721 actgctcatg ctgggcggga atctggaccc gacgatgggc gaggggcatc cgtttccgcc
     3781 gcagaaggat tgggacttca cgcagcacaa gccgttcaag gcggtgtatg acacgaatcg
     3841 ccgcagcgtg tatctgatga cgcagcggat ccagcggcat ccgctgatgg cgacgttcga
     3901 cggccccgat accggcgcga gcacgggcag tcggatcacc agtacgacga cgctgcaggc
     3961 gctctatttc ctgaacgacc cgctggtcca cgcgcaggcc aaccgcatcg ccgaccgcgt
     4021 ccgcaattca gccgacaacg acgccgggcg gatcgaccac gcctacaatc tgctctacag
     4081 ccgccccgcg accgaggacg agcaatcgct cgcggccggc tacttgtcca aactccgctc
     4141 ccaagccggc gacaaccccg aagccgcggc gtggcaaagc ctgatccgcg tgctgctgcg
     4201 gacgaatgag tatgtgtatg tggaataacg gctccggctc ggcgaagtac tgagtactga
     4261 gtactgagta ctgagtacct tgtgcgataa accaaccgat ccccgttgat ttcatcttgt
     4321 cgggcgtgca gccccccgct acatttccgc ccgacgattg ctctgccatg ttcgtgtttg
     4381 cggcgtcatt tgttggggag ccgatacgag caccgcaagg gagctgaaag tttgcctggc
     4441 cggcgcgtcc acctgagcag caacgctccg ttaacgcacc cacgggctgt ggctcccacc
     4501 ttttgcactg cgggctctca caaccacgcc ccacggcatt gcggtggagt acgtcgtcgt
     4561 ttccaggcgg cgaattgctt cttaagtggc cgggcgggaa gtctgcagtg cccggcgatg
     4621 tctcgccgcg tcatccgccg cgtattccag cgctaaattg tccgctgact tgcaatccga
     4681 attgccaggg cggaaaatgc ctagccagag gcgtcagtat acctatcccc aagggagctc
     4741 ccggagaatt gccatgaatc gaatcgtctt catcgccgta tcgagtgcct tgtgtctcgg
     4801 ctcgagtgga acgctggtgg cggagacgaa ggtcacgatc tccaaaacgc acctgtgctg
     4861 tccgcagtgc ctcactggtg ttgcagcggc tctcaaggat gtggacggag tcacccacaa
     4921 gtgcgatcag aaagccaaaa ccatcgagct tgccgccgag agcgatgcgg ccgcgcaaaa
     4981 ggcggtcgat gcgctcgcgg ctgccgggtt ttatggggcg accgatagcg ataccgtgaa
     5041 gtacaaagcg gtcgaagcgc cggagggcga attggagcgc ctggaagttg ccgggatcca
     5101 taactgctgc ggggcctgca ccgtggccat taagaaggcg atcgacacgg tcgatggcac
     5161 caccgcgaac accgtgaagg ccaaggagag caagtttgtc gtggaaggga agttcagcgc
     5221 cgagaagctg gtgcaggcgc tgctcgatgc gggcttttac gtgcaggtga agaagtaagc
     5281 tatgtcgtga gcacgtcagc gggcttgaag tcatcgccaa gaatgggcga tgacggccag
     5341 ccgagccagt tttgcagaag ggcggcatag acggcgcgga agtcggtgtg gaacttgaga
     5401 tcgccgtctt ccaggtcggc gaagctgggg tgcttgccga tcaagccgga ctttacccgg
     5461 tcgccgacga gaaagaccgg ggcggccgcg ccgtggtcgg tgccgcggct ggcgttttcg
     5521 ttcacgcgtc ggccgaactc gctgaacacg agcgtcgtga cgcggtcgag ttggccgtgt
     5581 gcctgcaggt cttccgcaaa ggcggccagc gcgccgccga gctgctgtaa gaggcctgag
     5641 tgagcagcgg cctggtcgga atgggtgtcg aagccatcga gggccacgta atagacgcga
     5701 gtggccaggc cggcgtcgat gagctgggcg atccgcttca gcttttcggc cagcggggtg
     5761 ccgggatagg cgacggaagt cttgtacgac tggcccgacg actcgatccg gcggctgact
     5821 tccagggccg aagtcgtccg cgtctggacg aacttcagca gatcgttttc tgcggcccgc
     5881 ggcgccgcgg cagccgactg aatggcggcg cggcgattgg cgtcgccccc cgtttcgagc
     5941 ttgaactggt cgagcgagcg aatcgacg
//
LOCUS       DASUGB010000505         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1445333, whole genome shotgun sequence.
ACCESSION   DASUGB010000505 DASUGB010000000
VERSION     DASUGB010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445333"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1097)
                     /gene="hisC"
                     /locus_tag="VFV87_09685"
     CDS             complement(24..1097)
                     /gene="hisC"
                     /locus_tag="VFV87_09685"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5104070.1"
                     /translation="MYVRPEIAAMAGYTPGEQPQGVSKFIKLNTNENPYPPPAGIRRA
                     IEEVLDRGLQKYPDPMASAFRTRAAELLGLPGKDWILAGNGSDDVLTICTRAFVGQGE
                     LLRLPYPSYILYRTLAQLQGARWEEVRFNADWSLPAAFGDSADGLKLVFLPNPNSPTG
                     TMISREAVLKFAERLLCPLLVDEAYVDFADWNCVDLVRQNEKILVSRSLSKSYGLAGL
                     RFGYLIGQPQIIEQLVKVKDSYNCDALSIAAATAAINDTAWLAENRRRIIATRSRLTT
                     GLQQLGFEVIPSQANFVWCTHARHAAKALYEQLKAAHVLVRFMSYPGWSDGLRISVGS
                     DEQIDALLATLASIQNPKSKIQN"
     gene            complement(1145..2530)
                     /gene="hisD"
                     /locus_tag="VFV87_09690"
     CDS             complement(1145..2530)
                     /gene="hisD"
                     /locus_tag="VFV87_09690"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5104071.1"
                     /translation="MNTPFSIQRIDTRRGNVRAALDSLRQKLSPAGSVVSEAGRRRTM
                     EVFGEPLSPQQVVERICGDVKARGLEAVLEYSRKLDKAELTAESIRVSPQELAAAHAA
                     ADPAFLATVRRIRENILEFQRAILHRDAEVTRPGVVLKQRYQPLERVGICVPGGAAAY
                     PSTVLMTAVPAQAAGVPEIAVVAPPTKFGANNPVVLATCHELGIREVYRVGGAQGVAM
                     LAYGIESVPKVDKIVGPGNLFVALAKRHVYGEVDIDSIAGPSEVVVIADERARPDFTA
                     ADMLAQAEHAPGASILVTWSEQMLEATAAELQEQVATLSRCEETIQSLRDFGCLILVR
                     DEDEACEITDLIAPEHLHVAIDNAERLLPKLRNAGATFLGNYSPVALGDYAAGPSHVL
                     PTGGTARWASGLSANQFLRSSSVIQYSEEALHGIAADVMRMAEIEGLTAHRTSVAIRN
                     SEFKIQNSESK"
     gene            2648..3730
                     /gene="tdh"
                     /locus_tag="VFV87_09695"
     CDS             2648..3730
                     /gene="tdh"
                     /locus_tag="VFV87_09695"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX5104072.1"
                     /translation="MSTSPRDLPETMLAVRKLKSEPGLWLQNDTPVPKIGPRDALVTV
                     THTGICGTDRHIYEWDAWSRGRVKLGITTGHEFVGRVVAVGSAVTRTAVGQRVSAEGH
                     IGCGVCQPCRTGNGHICEKVDILGIDTEGCFAEYVAVPEENVWPVHPDIPDHIAAIFD
                     PLGNAMHTVMAAGVSGRSVLITGVGIIGLMAVTISRAAGAGKILVADVSPRRLELAKG
                     LGADVAFDSRDENWSQKARAETHDQGPEVFLEMSGNAKAIRQGFAALRNGGTAALLGL
                     PAEPVTLDLPNDIIFKGATVLGINGRKMFETWYQVESFLLSGRLNIEPIVTHRLPLTE
                     FERGLKMMQAGEAIKVVLEVGRCGNI"
     gene            3797..3967
                     /locus_tag="VFV87_09700"
     CDS             3797..3967
                     /locus_tag="VFV87_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104073.1"
                     /translation="MSDGAKSEQLVPAVRDKDVLGQSGRSTTTTIKEAKAVIEKVRED
                     QRSRPEQQKPTK"
     gene            4042..4518
                     /locus_tag="VFV87_09705"
     CDS             4042..4518
                     /locus_tag="VFV87_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104074.1"
                     /translation="MIAAVAALVAIGLGADQLRKVVDALRMSGLGAILQLETDIHARK
                     QRVVESATEILRANLDLETEPNQIRKQRIEQEILILSKKHDAFTEHWLNSADRLAFVI
                     LYCLRKRYFRDRDWKSEYYAYMKDLVDSNPEWFGTHTDYNNVLKLIAKWERPPRRR"
     gene            4532..>4960
                     /locus_tag="VFV87_09710"
     CDS             4532..>4960
                     /locus_tag="VFV87_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5104075.1"
                     /translation="MPNASFDARARDLLKSLADSRQLKSFYSVTGPLGPTAMVEGRGE
                     VIVLCSNNYLGLASHPEVVAAGIEGLKKYGAGTASVRFICGTLDCHRQIEQTIARFVG
                     TQAALSYVSCWNANEAVFPTLVGPDDCILSDELNHASIIDS"
BASE COUNT          968 a         1555 c         1506 g          931 t
ORIGIN      
        1 cgattttggc ggtgcggggc atttcaattt tggattttgg atttcggatt ttggattgag
       61 gcaagtgtgg ccaatagcgc gtcgatctgc tcatcgctcc cgacgctgat ccgcagcccg
      121 tcgctccagc ccggatagct cataaaccga accagcacat gcgccgcctt gagctgctcg
      181 tacagcgcct tggcggcgtg cctggcgtgc gtacaccaca caaagttagc ttgcgaaggg
      241 atgacctcga agcccaattg ctgcagtccc gtcgtaagtc ttgagcgggt cgcgatgatc
      301 ctgcggcggt tttcggccag ccaggcagtg tcgttgattg ccgccgttgc cgccgcgatc
      361 gaaagggcgt cgcagttgta ggagtccttc accttgacga gctgctcgat gatctgcggc
      421 tgaccgatta agtagccgaa gcggaggccg gccaggccat aagatttact caagctgcgg
      481 ctgacgagga tcttttcgtt ttgccttacc aggtccacgc agttccagtc ggcaaaatcg
      541 acgtaggctt cgtccaccag cagcgggcag agcaatcgct cggcgaattt cagaaccgcc
      601 tcgcggctga tcattgtgcc ggtcgggctg ttgggattgg gcaggaagac gagcttcagg
      661 ccgtcggcgc tgtcgccgaa cgccgccggc agcgaccaat cagcattgaa ccgcacctcc
      721 tcccaccgcg ctccttgaag ctgggccagc gtgcgataca ggatgtagct cggatacggc
      781 agtcgcagca gttccccctg gccaacaaac gcccgcgtgc agatcgtgag aacgtcgtcg
      841 ctgccgttgc ccgccagaat ccagtctttt ccaggcaatc cgagcaattc agcggctcgc
      901 gtgcggaagg cgcttgccat cggatcggga tacttctgca agccgcggtc cagcacctct
      961 tcaatcgccc gccgaatccc tgccggcggc gggtacggat tctcgttcgt gttgagcttg
     1021 atgaacttgc tcaccccctg cggctgctcg ccgggcgtat agccagccat cgcggcgatt
     1081 tcggggcgaa cgtacataga aatgcagatt tcagaatgca gagttggaat tgaaatcacg
     1141 tttgctactt cgattctgaa ttctgaatct tgaattctga attccgaatc gcgacactcg
     1201 tacggtgtgc cgtcaggcct tcgatttccg ccattcgcat gacgtcggcg gcaattccat
     1261 gcaacgcctc ttccgagtat tgaatgacgc tgctgctccg cagaaactga ttcgcggaca
     1321 agcctgaggc ccagcgggcg gtgccgccgg tggggagcac gtgcgacggg ccggcggcat
     1381 agtcgccgag ggcgacgggg ctgtaattgc ccaggaacgt cgccccggca tttcgtaatt
     1441 tcggcagcag acgttcggca ttgtcgatgg cgacgtgcag gtgctcgggc gcgatcaggt
     1501 cggtgatctc gcacgcttcg tcctcgtcgc gcaccaggat caagcagcca aagtcgcgca
     1561 ggctctggat cgtctcttca cagcgcgaga gggtggcgac ttgctcttgc agctcggctg
     1621 ccgtcgcttc cagcatctgc tcgctccaag tgacgagaat gctcgccccg ggcgcgtgct
     1681 cggcctgagc cagcatatcg gccgcagtga aatcgggccg cgcccgctcg tcggcgatca
     1741 cgacgacctc gctgggccca gcaatcgagt caatgtcgac ctccccataa acgtgccgct
     1801 tggcgagggc cacgaacaag tttcccgggc cgacgatctt atcgactttc ggcacggatt
     1861 cgatgccgta ggccagcatc gccacgcctt gcgccccgcc gacccgatag acctcgcgaa
     1921 tgccgagctc gtggcacgtc gccagcacga ccgggttgtt cgccccgaac ttcgtcggcg
     1981 gcgcgacgac cgcaatctcc ggcacacccg ccgcctgcgc cggaacggcc gtcatcagca
     2041 ccgtcgaggg ataagccgcc gctccgccgg gcacgcaaat gcccacacgt tcgagcggct
     2101 gataacgctg cttgagcaca acccccggcc gcgtgacctc ggcatcgcga tgtaaaatgg
     2161 cccgctgaaa ctcgaggata ttctcgcgaa tcctccgcac tgtggccaga aacgccggat
     2221 cagccgccgc atgagccgct gccagctcct gcggcgagac gcgaatcgat tcagccgtga
     2281 gttccgcctt gtccagcttc cgcgaatact ccagcacggc ttccagcccg cgagctttca
     2341 catcgccgca aatccgctcc accacctgct gcggcgagag cggctcgcca aagacctcca
     2401 tcgtccgccg ccggcccgct tcgctgacga cactcccggc cggactcagc ttctgtcgca
     2461 aggaatccag ggccgcgcgc acgttgccgc ggcgagtgtc gatgcgctgg atggaaaacg
     2521 gcgtattcat gtggtcgcgg cttagcaagc cctaggacaa ggctctacgc cattgtgttc
     2581 gcggcccccc gactgcggaa ggccatctgc ccgtgtcgat gttgccccgc gcgtatagaa
     2641 tctcgccatg agcacctctc cccgcgacct gcctgaaacg atgctcgccg tccgcaagct
     2701 caagagcgag ccgggcttgt ggctgcagaa cgacacgccc gtgccgaaaa tcggcccgcg
     2761 tgacgctctt gtcaccgtca cccacaccgg catctgcggc accgatcggc acatttacga
     2821 gtgggacgcc tggagccgcg gccgcgtgaa gctcggcatc acgacggggc acgaattcgt
     2881 cggccgggtc gtcgccgtgg gcagtgccgt cacgcggacc gccgtcggcc agcgggtcag
     2941 tgccgagggg cacatcggct gcggggtctg ccagccctgc cgcacgggca acggccacat
     3001 ctgcgagaag gtcgatatcc tgggcatcga cacggagggt tgcttcgcgg aatacgtcgc
     3061 cgtgccggaa gagaacgtct ggccggtcca cccggacatt cccgaccaca ttgcagcgat
     3121 tttcgacccg ctcggcaacg cgatgcatac ggtcatggcc gccggcgtca gcggccgcag
     3181 cgtgctgatc acgggcgtcg gcattatcgg cctgatggcc gtcaccatca gccgggccgc
     3241 cggtgccggc aagattctcg tcgccgacgt cagcccccgc cgcttggaac tggccaaggg
     3301 cctgggcgcc gatgtcgcct tcgactcgcg cgacgaaaac tggtcgcaaa aagcccgcgc
     3361 cgaaacgcac gatcaaggcc ccgaagtctt cctcgaaatg agcggcaacg ccaaggcgat
     3421 ccgtcagggc ttcgccgccc taaggaacgg cggtaccgcc gcgctcctcg gcctgcctgc
     3481 cgagccggtc acgctggatt tacccaacga catcatcttc aaaggcgcca cggtcctggg
     3541 tatcaacggc cgcaagatgt tcgaaacctg gtaccaggtc gaaagctttc tcctctccgg
     3601 ccggctgaat atcgagccca tcgtcaccca ccgcctgccg ctgaccgagt ttgaacgtgg
     3661 cttgaagatg atgcaggcgg gggaggcgat caaagtggtg ctggaagttg gacgctgtgg
     3721 gaatatttga ttgcaactgc cgacacgata tgattgtttg tttggatgga taatgggccg
     3781 aaacggaggc taaccaatgt cggacggtgc aaaatcggaa caactcgtac ccgcggtccg
     3841 ggataaggac gttctcggtc aatcaggtcg ctcaaccaca acgacgatca aggaagcaaa
     3901 ggcggtaatc gaaaaggtcc gcgaagatca gcggtcaaga cctgaacagc aaaagccgac
     3961 caaatagccg accctcaagc ggaatcgctt atgcttcttg cgcaagccac cgccgccgat
     4021 tagttcggca gcatcggtag cgtgattgcc gcagtagcag cgttggtcgc gattggcctt
     4081 ggcgccgatc agcttcgcaa agtggtcgat gccttgcgaa tgtcggggct gggcgcgatt
     4141 cttcaacttg aaacggacat ccacgcacgc aaacagcgag tcgtcgagtc cgcgaccgag
     4201 attctgcgag ccaatctgga tttggaaacc gaacccaacc aaattcggaa gcagcgaatc
     4261 gagcaagaaa ttttgattct gagcaagaag cacgatgcat tcacggagca ttggctcaat
     4321 tctgctgatc gcctcgcatt cgtcatcttg tattgccttc gcaagcgata tttccgcgac
     4381 cgtgactgga agtcggagta ctacgcctac atgaaggatt tggtggacag caaccccgag
     4441 tggttcggaa cgcataccga ctacaacaac gtcctcaagc tcattgccaa atgggaacgt
     4501 ccgccaagaa ggcgatgacg atcgagtaat catgcccaac gcatcgtttg acgcccgcgc
     4561 ccgcgacctg ttgaagtcgc ttgccgattc gcggcagctc aagagttttt acagtgtgac
     4621 ggggccgctg gggccgacgg cgatggtcga ggggcggggc gaggtgattg tgctctgctc
     4681 gaacaactac ctggggctgg cgagtcatcc ggaggtggtc gcggcgggga ttgaggggct
     4741 gaagaagtac ggcgcgggga cggcttcagt gcggttcatc tgcggcacgc tcgactgcca
     4801 ccggcagatc gagcagacga tcgcccggtt tgtcggcacg caggcggcgc tcagttatgt
     4861 gagctgctgg aatgcaaatg aggcggtctt tccgacgctg gtcgggccgg acgattgcat
     4921 cctttccgac gagctgaacc acgccagcat catcgacagc
//
LOCUS       DASUGB010000506         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1862876, whole genome shotgun sequence.
ACCESSION   DASUGB010000506 DASUGB010000000
VERSION     DASUGB010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862876"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="VFV87_09715"
     CDS             <1..1326
                     /locus_tag="VFV87_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104076.1"
                     /translation="DASGYCHDACIAGRSVIVLMDIREFGRGCSLAPGLFVLAVLALP
                     GLQAQAGDWPQILGANRDGKAAADEKLLESWPAAGPKVLWRQKLGSGYAGVAVAEGKV
                     IAFHRVGDVERVEAFDSATGKSLWKADFEANYSGGIDADKGPRCVPLVAGEKVYVFGA
                     AGDLHAVGLADGKRLWTRNLYADYEGSEGYFGAGSTPILVGGKLIVNVGGRGAGLVAL
                     DPATGKTAWQATDEAASYSSPTAVRFNSGDRALFITRLNCVLVDPKTGNAQTLVPFGQ
                     RGPTVNAATPLIIGGKMFLTSSYGVGALYSKFDTAGSQEVWANDDTLSSQYATPVEQG
                     GFLYGIHGREDVGVAELRCVEAATGKVRWSQADYGVAHLILADGKLLIQSVEGRIALA
                     AADSGKYRELATHQLTREPTRALPALANGRLCVRTGSGGGELICLQVGR"
     gene            complement(1413..1694)
                     /locus_tag="VFV87_09720"
     CDS             complement(1413..1694)
                     /locus_tag="VFV87_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX5104077.1"
                     /translation="MSRTRIPLSKEDEIARLQKERLEMSTAEIGLTVRTTNCLEERGI
                     FTVRDLLNSTPDQLLSIANFGEKTLEEVYKSLEKIGFYRASKKKEAALV"
     gene            2009..>2781
                     /locus_tag="VFV87_09725"
     CDS             2009..>2781
                     /locus_tag="VFV87_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5104078.1"
                     /translation="MDTISVSRPADIFESLTPAQLEAVRHVDGPLLILAGPGSGKTRV
                     VTHRVANLLQQGISGRNIVALTFTNKAADEMKARLARLVALPTVWMGTFHRFCARLLR
                     IYGPHIGLSENFSIYDVEDSRKMLVEALVDEGIELTHASPDAVANAISWAKNNLMGPD
                     EYSPRRGHAIGGIVQRVYPAYQRRMLQANAVDFDDLLLHVAKLLKDNPDIRARLDDRY
                     RYILVDEYQDTNLAQYAIVRALSIDFPNLAVTGDPDQSI"
BASE COUNT          482 a          895 c          888 g          516 t
ORIGIN      
        1 gacgcttcgg gttactgtca cgatgcctgc attgctggaa ggagcgtgat cgtgctaatg
       61 gacattcgtg aatttggtcg cggctgctcg ctggcgccgg gactcttcgt gttggcagtg
      121 ctcgcgctgc caggattgca ggctcaggcg ggcgactggc cgcagattct tggcgccaat
      181 cgcgacggca aggctgctgc ggacgagaaa ctgctcgaat cctggccggc ggccgggccg
      241 aaggtgctct ggcggcaaaa gctcggcagt ggctatgccg gcgtcgccgt ggccgagggg
      301 aaggtcatcg ccttccaccg cgtgggcgat gtcgagcggg tcgaagcctt tgattcagcc
      361 acggggaagt cactttggaa ggctgacttt gaagcgaatt atagcggcgg catcgatgcc
      421 gacaaaggtc cgcgctgcgt gccgctcgtc gctggcgaaa aggtttatgt cttcggagcg
      481 gccggcgact tgcacgccgt cggcctcgcg gacggcaaac gactttggac gcggaacctg
      541 tatgcggact atgagggaag cgaaggctac ttcggtgcgg gaagcacgcc gattctcgtc
      601 ggcggcaagc tgatcgtgaa tgtcggcggc cgcggcgcag ggctcgtggc tctcgatccg
      661 gccaccggca agaccgcctg gcaagccacc gacgaagcgg ccagttattc atcgccgacg
      721 gcggtgagat tcaactcagg cgaccgggcg ctgtttatca cccggctcaa ttgcgtgctc
      781 gtcgatccga aaaccgggaa tgcgcagacg ctggttccct ttggccagcg cggtccgacg
      841 gtgaacgccg ccacgccatt gatcatcggc ggcaagatgt tcctcacgtc gagctatggc
      901 gtcggcgcct tgtattcgaa attcgacact gccggctcgc aggaggtctg ggccaacgac
      961 gatacgctca gcagccaata tgccacgccg gtcgaacagg gaggctttct ctacggcatt
     1021 cacggccgcg aggatgtggg cgtggccgag ctgcgctgcg tcgaagccgc gaccggcaag
     1081 gtccgctggt cgcaggccga ctatggcgtc gcccacctga tcctggccga cggcaagctc
     1141 ctcatccagt ccgtcgaggg ccgcatcgcg ctcgctgccg ccgattcggg caaatatcgc
     1201 gagctcgcca cgcaccagct cacgcgcgag ccaacccgcg ccctccccgc cctcgccaac
     1261 ggccgcctct gcgtccgcac cggcagcggc ggcggcgagc tgatctgcct gcaagtcggc
     1321 cgatagcgct gtagggaacg gtctccgtgc cgttccgaaa gtgtatcgcg accttgctcg
     1381 cagaacgccg cggagggcgt tccctacgga attcaaacca gcgccgcttc tttcttcttg
     1441 ctggcgcggt agaagccgat cttctcgagc gacttataaa cttcctccag cgtcttctcg
     1501 ccgaagttgg cgatcgagag gagctggtcg ggcgtgctgt tgaggaggtc gcggaccgtg
     1561 aagatgccgc gctcctccag gcagttcgtc gtgcggaccg tcaggccgat ctcggctgtg
     1621 ctcatctcca gccgttcttt ctgcaggcgg gcgatttcat cctctttgct gagcggaatg
     1681 cgggtacggc tcatggttcc ctccctggga cgtggtggaa attgtggggt aggcgccagc
     1741 gcctgcccgt caaagccgcg accagtggtc gcggttgggt gtgcagcgcc gcgacgaccg
     1801 gtcgcgggtt ttgcagggcc ggccagaggc ccaccccacg ttgcactcgg gcggaaagta
     1861 taacgaaccg ctgccgagtt cctagcaatt tgctggcaaa gttctgaaga atttttccgc
     1921 aagtgctagc aatgctgtga cagcacgtcc ggcctttgcc cggcattgct acaatagagc
     1981 tcgaaaggcc tctttcgcag ttccgcgtat ggacacgatc tcggtttcgc gccccgccga
     2041 tatcttcgaa tccctcaccc ccgctcagct cgaagcggtg cggcacgtgg atggcccgct
     2101 gctgattttg gccgggccgg ggagcggcaa gacgcgggtc gtgacgcatc gcgtcgccaa
     2161 tctgctgcag cagggcatca gcggacggaa catcgtcgcc ctcaccttca ccaacaaggc
     2221 ggccgacgaa atgaaggccc gcctcgcgcg gctcgtcgcc ctgccaacgg tctggatggg
     2281 cacgttccac cgcttctgtg cccgcctcct cagaatctat ggaccgcaca tcggcctctc
     2341 ggaaaacttc tcgatctacg acgtcgagga cagccgcaag atgctggtcg aggccctggt
     2401 cgatgaaggg atcgagctga cgcacgcctc gcccgacgcg gtggcgaatg cgatcagttg
     2461 ggccaagaac aacctgatgg ggccggacga gtattccccg cggcgcggcc acgcgatcgg
     2521 cggcatcgtt cagcgggtct atcccgccta ccagcggcgg atgctgcagg ccaacgccgt
     2581 cgatttcgac gacctcctct tgcacgtggc gaagctgctc aaggacaacc ccgacatccg
     2641 cgcccggctc gacgaccgct accgctacat cctcgtcgat gaataccagg acacgaacct
     2701 cgcccagtac gcgatcgtgc gggccctctc gatcgacttc ccgaatctcg ccgtcaccgg
     2761 cgatcccgac cagtcgatct a
//
LOCUS       DASUGB010000507         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_751858, whole genome shotgun sequence.
ACCESSION   DASUGB010000507 DASUGB010000000
VERSION     DASUGB010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751858"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..972
                     /locus_tag="VFV87_09730"
     CDS             370..972
                     /locus_tag="VFV87_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104079.1"
                     /translation="MNSSLAIETFTTHRQFLFSIAYRMLGSLFEAEDMVQEVWLRWQT
                     QDAAGIQSPKAWLRSAMKRLCIDQLRSARHQREDHYGVSLPEPSLPAADITTDSSMEL
                     EASLSMAFTLMQETLKPVERAVFLLRDVFDYHYADTAAIVGKAEANCRQIVRRARAKL
                     LSKATPTPPPNDQARYLAEQFLSAAATGEVKRFVALVKED"
     gene            1111..>2105
                     /locus_tag="VFV87_09735"
     CDS             1111..>2105
                     /locus_tag="VFV87_09735"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5104080.1"
                     /translation="MKMNIKIVVIGGTGLIGSKLVNILLQRGHEAVAAAPSSGVNTLT
                     GEGLAEALKGAQVVVDVANAPSWEDKAVMEFFETSGRNLLAGETAAGVKHHVALSVVG
                     TDRLLASGYFRAKMAQENLIKASSIPYTIVRSTQFFEFANGIAKNAAQGETVRLPAAL
                     MQPIAADDVAALLADVALGEPTNGTLEIAGPEPIRQDEFVRKYLKATGDARGVTTDPT
                     ARYYGLEVNDSSLVPGKHPRVGSIRFSEWLSRNAPQKTAPVANLSAKAARIWALLLCG
                     IALTAVCLTETAQGDSPANHSRAAGSKEAPEAKVTSLFSKDLADFPGKEGLMIVV"
BASE COUNT          458 a          586 c          645 g          416 t
ORIGIN      
        1 caggccttgc gccagatgac gcacaaggga gcagaagaaa tccgtcccgg tgaccgctgg
       61 aggtgccttg aatgatggcg cgcagcaatt cctcggaacg ctccggcggg gcgctgtcgt
      121 gcttgggcag agcgggattc atcaactcac caggctcgtt tttgaggact caatggggtg
      181 aggggagccg ccgcgtgtta ctgctcatcc cgccccttcg tgacaacact tcggcagcgc
      241 gacgaaggat tggcacgagc tgacgtcatc aactcttcac atcggggttc acaaagagct
      301 tttgcgggcc tttttgagga aagccggacg ttcccgacct tggcctgaaa ggtgcgattg
      361 ggggaaagca tgaactcgtc actcgcaatc gaaaccttca ccactcatcg ccaattcctg
      421 ttcagcatcg cataccgcat gctggggagt ttgttcgagg cggaagacat ggtgcaagag
      481 gtatggctgc gatggcaaac gcaggacgcg gccggaatcc aatcgccaaa ggcgtggctg
      541 cggtctgcca tgaagcgact gtgcattgat cagttgcgtt cagcgagaca tcaacgggag
      601 gatcattatg gagtctcgct gccagaaccc tcgctgccgg ccgcggacat tacgacggac
      661 agttccatgg aactggaggc ttccctgtcg atggcgttca cgctgatgca ggagacgctc
      721 aagccggtgg agcgtgccgt gttcttgctg cgcgacgtat tcgattacca ctacgccgac
      781 acggcggcca tagtcggcaa agccgaagcc aactgtcgcc agatagttcg gcgggccagg
      841 gcgaagttgc tttcgaaggc aacgccgacg ccgccgccaa acgaccaagc acgctatttg
      901 gcggagcaat ttctttccgc cgcggccacc ggcgaggtga agcgatttgt ggcgttggtc
      961 aaggaggact aatgcccaac gattccagaa agattgtgaa cgagtttgaa gctgtcacgg
     1021 gccaggaccg tggttcgtct ctgttgacgt tgtctcaaag cggtagacgc gggcaaacac
     1081 ttcaatgaaa tcgcagcaat ggattctgat atgaaaatga acatcaagat agtggtgatt
     1141 ggcggcactg gtttgatcgg atcaaaactg gtgaacattc ttttgcaacg ggggcatgaa
     1201 gcagtagcgg cagctccctc ctcgggcgtt aataccctca cgggtgaagg actggccgag
     1261 gccctcaagg gggctcaggt cgttgtggat gtcgccaacg cgccgtcgtg ggaagacaaa
     1321 gcggtgatgg aattctttga aacatccggc cgcaatctgc tcgcgggaga aacggccgct
     1381 ggtgtgaaac atcatgtcgc cctttcggtc gtcggtaccg accggctgct ggcgagcggc
     1441 tattttcgcg cgaagatggc tcaggagaac ctgataaaag cctcttcaat tccctacacc
     1501 atcgtccgct caacccagtt cttcgagttc gcgaacggca tcgccaaaaa cgctgcgcaa
     1561 ggtgaaaccg tgcgattgcc ggccgctctg atgcagccca tcgcggcgga tgacgtcgct
     1621 gccctattgg cggatgtcgc cctgggcgag ccaacgaatg gcacgctcga aatcgccggg
     1681 ccggaaccga tccgacagga tgagtttgtt cggaagtatc tgaaggcaac cggcgatgcg
     1741 cggggagtga cgaccgaccc caccgcccgc tactacggtc tcgaagtcaa tgattcaagc
     1801 ctcgtccccg ggaagcaccc gcgcgttggt tcaatccgct tttcggaatg gctcagccgc
     1861 aacgctccgc agaaaacggc gccggtcgcg aatctctcgg cgaaagcggc gagaatatgg
     1921 gcgctgcttc tttgcggaat tgcccttacg gccgtgtgct tgaccgaaac tgcacagggc
     1981 gattctcctg cgaaccactc cagggcggcc ggatcgaagg aggcccctga agcaaaagta
     2041 acgtcgctgt tctcgaaaga cctcgccgac tttccgggca aggaaggtct catgattgtg
     2101 gttga
//
LOCUS       DASUGB010000508         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_57121,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000508 DASUGB010000000
VERSION     DASUGB010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57121"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFV87_09740"
     CDS             <1..353
                     /locus_tag="VFV87_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104081.1"
                     /translation="SCYQGDRAIDAMSVLLDGTYLETATVRLEESPPKEHGLRFFSYP
                     TRNALDLLDHHAQCIAEVALETGSQPTVLEPGDFPGVMNYGRQLSLRSLYRQGVLEEL
                     PEFLRRTEAAAVGP"
     gene            428..667
                     /locus_tag="VFV87_09745"
     CDS             428..667
                     /locus_tag="VFV87_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104082.1"
                     /translation="MEQVLTGTVQGNTIVLDSLPAIANGEQVEVVLRAVAAKGVWGEG
                     IRDSAGGWAGYPELDAIMESIHAQRALERGSANGP"
     gene            664..1062
                     /locus_tag="VFV87_09750"
     CDS             664..1062
                     /locus_tag="VFV87_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5104083.1"
                     /translation="MSFLLDTDTCSAHFRRPGGLAHRFFQHSGSLFVSAVNLAELYTG
                     AYHVANPQPLLDKVAELLRDVTVLPFDANCAHSFGQVRGGLLQRGIAVPTADLMIGAT
                     ALTHNLTLVTHNTKDYQHIPGLRLEDWLTP"
     gene            complement(1073..>2118)
                     /gene="fbp"
                     /locus_tag="VFV87_09755"
     CDS             complement(1073..>2118)
                     /gene="fbp"
                     /locus_tag="VFV87_09755"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX5104084.1"
                     /translation="SLALRATMSAMSQPQLQLLTVQQHILEEQRRLYPDASGEFSWLL
                     SGITLATKIVAAQVRRAGLVGIVGVTRETNVQGEQVQKLDVIANQTVMQFLGNRGNVA
                     IMASEENAEPVVVERDRKRGKYVVVFDPLDGSSNIDVNVSVGTIFSILRREPDPSGTR
                     DVMADVLQPGLRQVAAGYVVYGSSTMLVYSAGNGVHGFTLDPSIGAYLMSHEQIRMPE
                     CGSIYSCNEANANSFPPAYRKYLDHLRSGAAGRPYSSRYIGSLVADFHRNLLKGGVFL
                     YPPTKSHPSGKLRLLYEGNPIAFLAEQAGGIATDGTRRILEIQPTSLHQRTPLVVGSR
                     EEMETLQRFLGEG"
BASE COUNT          372 a          637 c          713 g          396 t
ORIGIN      
        1 tgtcgtgcta tcagggcgat cgggcgatcg atgcgatgtc ggttctgctg gacgggacgt
       61 atctcgaaac ggcgacggtc cggctggagg aatcgccgcc gaaggagcac gggctgcgat
      121 tcttcagcta tcccacgcgc aatgccctcg atttgctcga ccatcacgcc cagtgcatcg
      181 cggaggtcgc cctggaaacc ggctcgcagc cgaccgtgct ggagccgggc gattttccag
      241 gcgtgatgaa ctacggccgg cagctttcgc tccgctcgct ctaccggcaa ggcgtgctgg
      301 aggagctgcc ggagtttctg cggcggacgg aagcggcggc tgtaggtccc tgacgcattc
      361 gacggacgaa gttccacgac gcgcacggct cgagccgctg gtataatgcg gccggagggc
      421 gaaaccgatg gaacaggttc tgaccggcac ggtgcagggg aatacgatcg ttcttgattc
      481 actgcctgcc attgcgaatg gcgagcaagt cgaggttgta ttgcgggctg tggcggccaa
      541 gggagtctgg ggcgagggaa ttcgggattc cgccggcggc tgggcgggct atccggaact
      601 ggacgcgatc atggagtcga tccacgcgca gcgggcactt gagcgcggtt ccgcgaatgg
      661 accatgagct tcctcttgga cacggacacg tgctcggctc attttcgcag gccgggaggg
      721 ctggcacacc gattctttca gcattccggc agcttgttcg tttccgccgt gaatctcgcc
      781 gaactctaca ccggcgccta tcacgttgcg aatccgcagc cgctgctgga taaggtggcg
      841 gaacttcttc gcgatgtcac ggtcctgcct tttgacgcga actgcgcaca ctcattcggg
      901 caagttcgcg gcggccttct gcagcgcggc atcgccgtac cgaccgcgga cttgatgatt
      961 ggggcgaccg ccttgaccca caacctgacc ctcgtcacgc acaacacgaa agactaccag
     1021 cacatccctg gactgcgcct ggaggattgg ctgacgccgt aacgatcgcc gcttaccctt
     1081 cgcccaaaaa tcgctgcagc gtttccatct cctcccggct gccgacgacc aggggcgtgc
     1141 gctggtggag cgaggtgggc tggatttcga ggatgcggcg cgtgccgtcg gtggcaatgc
     1201 cgccagcttg ttcggcgagg aaggcgatgg gattgccctc atagagcagg cggagcttgc
     1261 cgctggggtg cgatttcgtc ggcgggtaga ggaagacgcc gcctttgagc aggttgcgat
     1321 ggaaatcggc gacgagcgag ccgatgtacc gcgaggaata cggccgcccc gccgcgccgc
     1381 tgcggaggtg atccaggtat ttccgatagg ctggtgggaa gctgttggca ttggcctcgt
     1441 tgcacgagta gatcgagccg cattccggca tccgaatctg ctcgtggctc atcaaatacg
     1501 ccccgatcga tggatcgagc gtaaagccgt gaacgccgtt gccggcggaa tagacgagca
     1561 tcgtggagct gccatagacg acgtagccgg cggccacctg acggaggccc ggctggagca
     1621 cgtcggccat cacgtcgcgc gtgccggacg ggtcgggctc gcggcgtagg atggaaaaga
     1681 tcgtgccgac gctgacattc acgtcgatgt tgctggagcc gtcaagcggg tcgaagacga
     1741 cgacgtattt gcctcgcttg cgatcgcgct cgacaacgac cggctcggca ttctcttcgc
     1801 tggccataat ggcgacgttg ccgcggttcc cgagaaactg catgaccgtc tggttggcga
     1861 tcacgtcgag tttctgcacc tgctcgccct ggacgttcgt ctcgcgagtc acgccgacga
     1921 tgccgaccag cccggcccgc cgcacctggg cggcgacgat cttcgtcgcc agcgtgatgc
     1981 ccgacagcag ccaggaaaac tcgccgctgg cgtccggata cagccgccgc tgctcttcga
     2041 ggatgtgctg ctggacggtg aggagctgaa gttgaggctg cgacatggcg ctcatcgtag
     2101 cccgtagcgc aagcgagg
//
LOCUS       DASUGB010000509         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1307428, whole genome shotgun sequence.
ACCESSION   DASUGB010000509 DASUGB010000000
VERSION     DASUGB010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307428"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFV87_09760"
     CDS             complement(<1..1009)
                     /locus_tag="VFV87_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104085.1"
                     /translation="MAVSRTCDGLQRRDFLKVGAIGAGVGSLSLSGFLRLAAAGEVKD
                     AKAKSAIFINLQGGPSHMDTFDLKPNAPSEFRGQFNPIKTNVEGVEISEHLPKLAANA
                     DKFAILRGVTHTLGAHELGTEYVNTGSRPIPSLEYPGYGAVVTKELGGPKDLPPFVAI
                     PNSAQRPGFLGVQFAPLNTTGTPRPGMPYGVRGITLGNGLTVAEVERRQSLLADLDKT
                     FAAVESNSQLLTGLDRFTEQAHAIITSRRAREAFDVSKESPAFAKPFGETPFGMSCLL
                     GSRLVESGVRFVTISLGGWDTHTSNWTRLKTKLLPDLDTGLAALFSGLAEKGLLDTTT
                     VF"
     gene            complement(1147..>3462)
                     /locus_tag="VFV87_09765"
     CDS             complement(1147..>3462)
                     /locus_tag="VFV87_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5104086.1"
                     /translation="YKQGGWEYHVFRRWLEAGAKFEEDQVQKLVNLEVTPAEILFAAP
                     GEKINLKAVAVWPDGTREDVTCLCRFTSNSDQVATIDADGLVTATEPGDTHVVVSYDN
                     AVIAIPVIRPVTQLTGSKYPEVATPTKIDELVVQKLRKLGVVPSDLASDSEFLRRVYL
                     DVTGTLPTSAEVEAFLADTSANKRAATIDRLLETPAYAAWWTTKFCDYTGNNDTKLNN
                     LGPRGQISQWWYDWIYERIEQNQPYDKIAEGLVLASSREKDESYTEFCEAMSKISQKD
                     SDEKFADRETMPYYWARNNFRQPEERAIGFAYTFLGIRIQCAQCHKHPFDQWSKKDFD
                     AFKNFFTGVTLSQNTPRDKESREEYDKIMKELGLDSSLRNNELRNKLYAMLNEGKTIP
                     FPEVTVTPPRQAPNRGNNRRNNQPPAQTAAVLGGEQVDTQGLSDIRQPLMDWLRQKDN
                     PYFARAFVNRVWAGYFNVGIVQPPDDMSLANPPSNRPLLDYLAAQFIEHGYDLKWLHR
                     EILNSRTYQLTWQPNETNAKDERNFARSVPRRMPAEVAVDALAAAVASDEKAAGYLTS
                     LEGRAIAIAGSSARSNAGANARDSGFALQVFGRSIRESNCDCDRSMDASLLQTVYLQN
                     DTSVLNALEGGRDSWIGQLTKATPDRLTDGTRTDNVNLDREIARMKDRLKRAKDDGND
                     EQVKRIEDRLAELQKASGAKQEAEKSPGKLALAEPELVRLAYLRTVSRLPSQDEMDRC
                     LAYLAEADSPAAGAKGLLWSLINTKEFIVNH"
BASE COUNT          540 a         1060 c         1144 g          718 t
ORIGIN      
        1 cgaagacggt cgtcgtgtcg aggaggcctt tttcggcgag gccgctgaac agggcggcca
       61 atcccgtatc gaggtcgggc agcagcttcg tcttgaggcg ggtccagttc gaggtgtggg
      121 tgtcccagcc gccgagcgag atcgtgacga accgcacgcc cgattcgacc aatcgcgagc
      181 ccagcaagca gctcatgccg aagggtgttt cgccgaacgg cttggcgaag gcgggcgatt
      241 ccttgctgac gtcgaaggcc tcgcgggccc gcctgctggt gatgatcgcg tgggcctgct
      301 cggtgaaccg gtccaggccg gtcaggagct ggctgttcga ctccaccgcg gcaaacgtct
      361 tatcgaggtc ggccagcagg ctctggcggc gctcgacttc ggccacggtc aggccgttgc
      421 cgagcgtaat gccgcggacg ccgtagggca tgcccgggcg gggggtgccg gtcgtattga
      481 gcggtgcgaa ctgcacgccc aaaaagcccg gccgctgagc gctattcggg atcgccacga
      541 agggcggcag atccttcggc ccgccaagtt ccttggtcac taccgcgccg tagccggggt
      601 attccagcga cggaatcggc cggctgccgg tattgacata ctcggttccc agctcgtggg
      661 cgcccagcgt gtgggtcacg ccgcggagaa tcgcgaactt gtcggcgttg gccgccagct
      721 tcggcaggtg ttcgctgatc tcgacgcctt ccacgttcgt cttgatcggg ttgaactggc
      781 cgcggaactc gctgggagcg ttcggcttga gatcgaacgt gtccatgtgc gaggggccgc
      841 cctgcaggtt gatgaagatc gcgctcttgg ccttggcgtc tttcacttcg ccggccgcgg
      901 cgaggcggag aaagccggaa aggctcagac tgccgacgcc cgcgccgatc gcgccgacct
      961 tgaggaagtc gcgccgctga agtccgtcac acgtgcgaga aactgccatt gtgaggttcc
     1021 ttggggaatg gaaagtgatt tggggaagct ggtttgaggt tctgtaggga acggaccctg
     1081 tgccgttccg cgatcgacga tctattacgg acctggttcc cacgctctgc gtgggaacga
     1141 ggttgcctag tgattgacga tgaactcctt ggtgttgatg aggctccaga gcaggccctt
     1201 cgcgccggct gccggggagt cggcttcggc gagataggcc aggcagcgat ccatttcgtc
     1261 ctgcgatggc aggcggctga ccgtccgcag ataggccagg cggacgagct ccggctcggc
     1321 cagcgccagc ttgccaggcg acttctcggc ttcctgcttg gcgccggagg ccttttgcag
     1381 ttcggccagg cggtcttcaa tccgcttgac ctgttcatcg ttgccgtcgt ccttggcccg
     1441 cttgaggcgg tccttcatcc gtgcgatttc gcggtccagg ttcacgttgt cggtccgcgt
     1501 gccatccgtc aagcgatcgg gagttgcctt ggtgagctgc ccgatccacg aatcgcggcc
     1561 gccttccagg gcgttcagca cgctcgtgtc gttctgcaga tagaccgtct gcaacaggct
     1621 ggcgtccatc gagcggtcgc agtcgcagtt gctctcgcga atgctccgcc cgaaaacctg
     1681 cagggcaaag cccgaatcgc gggcgttggc accggcattg ctgcgggcgc tggagccggc
     1741 aattgcaatc gcgcggccct ccaggctcgt caaatagccg gccgccttct cgtccgaagc
     1801 gaccgccgcc gccagcgcat cgaccgctac ttccgcgggc atccgccgcg gcaccgagcg
     1861 ggcaaagttc cgctcgtcct tcgcgttcgt ctcgttcggc tgccacgtga gctgatacgt
     1921 gcggctgttc aggatctcgc ggtggagcca cttcaggtcg tagccgtgct cgataaactg
     1981 cgcggccagg tagtcgagca ggggcctgtt gctcggcgga ttggcgaggc tcatatcgtc
     2041 cggcggctgg acgatgccga cgttgaagta accggcccag acgcggttga cgaaggcccg
     2101 ggcgaagtac gggttgtcct tctgccgcag ccaatccata agcggttggc ggatatcgga
     2161 caggccttgc gtatcgactt gctccccgcc cagaaccgcg gccgtttggg ccggcggctg
     2221 gttgttgcgg cggttgttgc cgcggttggg ggcttgccgc ggcggcgtga cggtgacctc
     2281 cgggaaggga atcgtcttgc cttcgttcag catggcgtac agcttgttcc gcaactcgtt
     2341 gttgcggagg ctggagtcga gccccagctc cttcatgatc ttgtcgtatt cttcgcgcga
     2401 ctctttatcg cgcggcgtgt tctgcgacaa cgtcacaccc gtaaagaagt tcttgaacgc
     2461 gtcaaaatcc ttcttcgacc actgatcgaa cggatgcttg tggcactggg cgcactggat
     2521 gcggatgccc aaaaatgtat aggcaaagcc gatcgcccgc tcttccggct ggcggaaatt
     2581 gttgcgggcc cagtaatagg gcatcgtctc gcggtcggcg aacttctcgt cgctgtcctt
     2641 ctgcgaaatc ttgctcatcg cctcgcagaa ctcggtgtag ctctcgtcct tctcgcggct
     2701 ggaggcgagc acgaggccct cggcgatctt gtcatagggc tggttctgtt cgattcgctc
     2761 gtaaatccag tcgtaccacc actgcgaaat ctgcccgcgt ggaccgagat tgttcagctt
     2821 cgtgtcgttg ttgccggtgt agtcgcagaa cttcgtcgtc caccaggcgg cgtaggccgg
     2881 cgtttcgagc agccggtcga tcgttgcggc ccgcttgttg gcggacgtgt cggccaggaa
     2941 ggcttccact tcggccgaag tcggcagcgt gccggtcacg tcgagataaa cgcggcgcag
     3001 gaattccgaa tcgctcgcga ggtccgaggg gacgactccg agcttccgca gcttctggac
     3061 gaccagctcg tcgatcttcg tgggcgtggc gacttcggga tacttgctgc ccgtaagctg
     3121 cgtgacggga cgaatcaccg gaatggcgat taccgcattg tcgtacgaaa cgaccacgtg
     3181 cgtgtcgccc ggctcggttg ccgtgaccaa gccatcggcg tcgatggttg ccacttgatc
     3241 gctgttgctc gtgaagcggc acaggcaggt aacgtcctcg cgcgtgccgt ccggccacac
     3301 ggcgaccgcc ttcaggttga tcttctctcc gggggcggcg aacaggatct cggccggcgt
     3361 gacttcgagg ttcaccagct tttggacctg gtcttcctcg aacttggccc cggcttcgag
     3421 ccagcgacga aagacgtgat attcccagcc cccctgctta ta
//
LOCUS       DASUGB010000510         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1863207, whole genome shotgun sequence.
ACCESSION   DASUGB010000510 DASUGB010000000
VERSION     DASUGB010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863207"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /gene="glgB"
                     /locus_tag="VFV87_09770"
     CDS             complement(<1..1474)
                     /gene="glgB"
                     /locus_tag="VFV87_09770"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX5104087.1"
                     /translation="MRTSVSLSAVEALLDLRLHAPRQVLGPQTIWDRGRKMLAVRAFL
                     PDSRQAWVLDPAEQKPARPMRRIHPAGLFEAICPESELPPGSHYQLRASDHEGQMQTM
                     HDPYAFAPYLSDFDLHLFGEGKMLQCYEKFGAQVREIDGVRGTNFAVWAPNAQSVSLV
                     GDFNRWDGRRHPMMHHQTGGIWELFVPGIEPGQKYKYRVRGRYGEVIDKSDPFGFAAE
                     LPPCTASIVSDLSRFRWNDHAWMDRRAHTNQLERPIAIYEVHLGSWRRGGERHHGWLN
                     YRDLAHQLVAYCQEMGYTHIELLPVSEHPYSGSWGYQTVGYYSVTSRYGTPEDFAYFV
                     DHCHRHDIGVILDWVPAHFPKDAHGLRRFDGTACYEHEDPRKGEHPDWGTMIFNYGRH
                     EVRNFLLGNALFWLDKYHIDGLRVDAVASMLYLDYSRKQGEWLPNQHGGRENLEAISL
                     LQEFNIHAHGNHPGVLTIAEESTAWGGVSRPTYTGGLGFSL"
     gene            complement(1522..2187)
                     /locus_tag="VFV87_09775"
     CDS             complement(1522..2187)
                     /locus_tag="VFV87_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEX5104088.1"
                     /translation="MRAPHFLLVSQAAVQGSGQVYSGRWKFELRTPDGLACLAAEDEE
                     AESSAERLELLAIVRGLESLDEPARVTLVSAGRSVSRSLRYGLSQWRENDWQWERYGQ
                     LTPVKNGDLWQRIDRAMTIHQVECREATAAPDDLAALNNVAAPLDARPEAAEVRIIHH
                     RGRRLRIDRGKAARMKAEGGRMKATEVHGNSISLVGWLGRLSLFWISALVRRLPGLSG
                     QTP"
     gene            2403..3092
                     /locus_tag="VFV87_09780"
     CDS             2403..3092
                     /locus_tag="VFV87_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104089.1"
                     /translation="MSSELPSQPDPAPPRLAPLEEDRPRLEDEPLDAIVFDVHPALAP
                     AHSVYKYPYGPAGDNPFQVRPEGIYRKPPVFSFQFMEVPADYVVPQRFGLSAILGIMT
                     ALAVLFGIMRWLEAHPFWYLFLGAQTVVICLVQMFHGKTPRKASAIAGAILAPLFILG
                     WGSWSRGPNEPGLVLCLVVMAVPFGGFLGYLNGTCAAGIFLVMDKLEPYLQGQRPLIP
                     AWRSSPTLPAS"
     gene            3089..3358
                     /locus_tag="VFV87_09785"
     CDS             3089..3358
                     /locus_tag="VFV87_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104090.1"
                     /translation="MTALPGVPVFNCIVNVSPPGQQAPCRAQVANLAGISASGPSERE
                     ALAKIVPQFKAAVARLHAANEPIPWIDPPMPPAAGELQRFIAVHL"
     gene            3556..3822
                     /locus_tag="VFV87_09790"
     CDS             3556..3822
                     /locus_tag="VFV87_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5104091.1"
                     /translation="MDRQRLRTTLLDLLEQETWERPETFTDDVKIREELKLDSVDLLG
                     VMLRAESTLGVSLDSRDFDKIATVGDLLDTIQRKLAAKPQNKAA"
     gene            3824..>4360
                     /locus_tag="VFV87_09795"
     CDS             3824..>4360
                     /locus_tag="VFV87_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104092.1"
                     /translation="MFTAGQDVALEAAVVAAGSSAVRLGDFAPLRKEPPRFGGVELAG
                     HFLKHADEQTIVALKAVGRAIERRRLDPRQMTDWAIVAAPRYIGHLAGASILDRFARQ
                     GGSGVPPHAVAQNSLHSISGAASIVLGLHGPNVGVGGGPWAIGDGLAAALTLFDASRV
                     PGVWLLVTQFEPELIPDEA"
BASE COUNT          732 a         1488 c         1361 g          779 t
ORIGIN      
        1 tcaggctgaa gccgaggccg cccgtgtaag tcggccgcga gacgcccccc caggcggtcg
       61 attcctcggc gatcgtcaag acgcccggat ggttgccgtg ggcgtggatg ttgaactcct
      121 gcaacagcga gatcgcctcc aggttttccc gcccgccgtg ctggttgggc agccattccc
      181 cctgcttgcg gctgtagtcg agatagagca tggaggcgac ggcatcgacc cgcaggccgt
      241 cgatgtggta cttgtcgagc cagaacaggg cgttgccgag caggaagttg cgcacctcgt
      301 ggcggccgta gttgaaaatc atcgtgcccc agtcggggtg ctcgcctttg cggggatctt
      361 cgtgctcgta gcaggccgtg ccgtcgaacc gccgcaggcc gtgggcgtcc ttgggaaagt
      421 gggccggcac ccagtcgaga atcactccaa tatcgtgccg gtggcagtga tcgacgaaat
      481 aggcgaaatc ttcgggcgtg ccataccggc tggtgaccga gtagtagccg actgtctgat
      541 agccccatga gccggaatac gggtgctcgc tgacgggcaa gagctcgatg tgggtgtagc
      601 ccatctcctg gcaataggca acaagctggt gggccaggtc gcggtagttg agccaaccgt
      661 gatgcctctc gccgccgcgg cgccagcttc ccaggtggac ttcgtagatg gcgatcggcc
      721 gctcgagctg gttcgtatgg gcgcggcggt ccatccaggc gtggtcgttc cagcgaaacc
      781 gcgacaggtc ggacacgatc gacgccgtac acggcggcaa ttcggcagcg aacccgaatg
      841 gatcgctctt gtcgatcact tcgccgtagc ggccgcggac gcggtattta tatttctgcc
      901 ccggctcgat gcccggaacg aacagctccc agatgccgcc ggtctggtgg tgcatcatcg
      961 ggtgccgccg gccgtcccag cggttgaaat cgcccaccag gctgacgctc tgcgcgttgg
     1021 gcgcccaaac ggcgaaattc gtgccccgca cgccgtcgat ctcgcgcacc tgcgccccga
     1081 acttctcgta gcactgcagc atcttcccct cgccgaagag gtgcaggtcg aagtcggaca
     1141 gatagggcgc gaaagcgtac ggatcgtgca tggtctgcat ctggccctcg tggtcgctgg
     1201 ctcgcagttg atagtggctg cccggcggca attcgctctc gggacaaatc gcctcgaaga
     1261 gtcccgcagg gtggatgcgc cgcatgggac gcgctggctt ttgctccgcc ggatcgagca
     1321 cccaagcctg gcggctgtcg ggcaaaaaag cccgcaccgc cagcatttta cgcccgcggt
     1381 cccaaatcgt ctgcgggcca aggacctggc gcggcgcgtg cagccgcagg tccaataggg
     1441 cctcgacagc cgagagcgaa acgctcgtac gcactgatag actccttcaa ggtgcagctt
     1501 cctgaaaacc aacacggcgg gctacggcgt ctggccgctt agccccggca gtcgccggac
     1561 cagcgccgag atccaaaaca aactcagtcg ccccagccaa ccgactagcg aaatggaatt
     1621 cccatgcact tccgtcgcct tcatccttcc gccttccgcc ttcatcctgg ctgcctttcc
     1681 cctgtcgatc ctcagccgcc gcccgcgatg atggatgatc cgcacctccg cggcttcggg
     1741 ccgtgcgtcg aggggagcgg caacgttgtt aagggcagcc aggtcgtcgg gggcggcggt
     1801 cgcctcgcgg cactcgacct ggtggatcgt catcgcccgg tcgatccgct gccacaggtc
     1861 gccgttcttc acgggcgtga gctggccata ccgctcccac tgccagtcat tttcgcgcca
     1921 ctgggacagg ccgtagcgca gcgagcggct gaccgagcgg cccgccgaaa caagcgtcac
     1981 gcgcgcaggc tcgtcgagcg attccaggcc ccggacgatg gccaacagct ccagccgttc
     2041 ggccgacgat tcggcctctt cgtcctcggc ggccaggcag gccaggccgt cgggagtgcg
     2101 cagctcgaat ttccagcggc cggaatagac ctggccgctg ccctgcaccg ccgcctgcga
     2161 aacaagcaga aaatgaggcg cccgcacgct cattgctcag aattaccgcc tgagaggcaa
     2221 ggactcaaat ccgttccgac actgcgggcg tcccttgccc cacaaaccac ccggccggca
     2281 ccgcgatccc ggccaccgat cacatcgaca atctcaccgc ggcctctcta gttttcccgc
     2341 gccgcacgat ttgtcacgcg gccgcttgga tacagagccg ccgcgcagca caataacgat
     2401 taatgtcctc ggaacttccg tcacagccgg accccgcgcc gcctcggctc gcgccactgg
     2461 aggaggatcg tccgcgcctc gaagacgagc cgctggacgc gatcgtcttc gacgtgcatc
     2521 cggcactggc gcctgcccac tccgtgtaca agtatccgta cggcccggcc ggcgacaatc
     2581 cattccaggt ccgtcccgag ggcatttacc gaaagccgcc ggtcttttct ttccaattta
     2641 tggaagtgcc ggccgactat gtcgtcccgc agcggttcgg cctgtcggcc atcctgggca
     2701 tcatgaccgc gctggccgtc ctgttcggca tcatgcgctg gctggaagcc catcccttct
     2761 ggtacttgtt cctgggggct cagacggtag tgatttgcct ggttcagatg ttccacggca
     2821 aaacgccgcg caaggcttcg gccattgccg gcgccatcct ggcgccgctg ttcatcctgg
     2881 ggtggggaag ctggtcccgc gggcccaacg agccggggct ggtgctgtgt ctggtggtta
     2941 tggccgtccc ctttggcggt tttttgggct acctgaacgg cacttgtgcc gccggcatat
     3001 tcctggtgat ggacaagctg gagccttact tacaaggcca acggccgctc atccccgcct
     3061 ggcgatcgtc gcccaccctg cctgcatcat gaccgccctc cctggtgtac cggtctttaa
     3121 ctgcatcgtg aatgtctcgc cgccgggcca gcaggcaccg tgtagggcgc aggtcgcgaa
     3181 cctggccggc atttcggcca gcggcccgag cgagcgcgaa gccctggcca agatcgtgcc
     3241 gcagttcaaa gccgccgtgg cccgcctgca cgctgccaac gagcccatcc cctggatcga
     3301 cccgccaatg ccgccagcgg ccggcgagct ccagcgcttt attgccgtgc atctgtagtc
     3361 ctgccaccca tatcgttggt ccggcagcag ttctcgtccg acggaggacc gtcggaccac
     3421 attcggacgg cgccttgggc gtgccaaatc gcccttgggc cagcccacgc catctgctac
     3481 cttcccgcct cggcgcggct cggcagtgag aaactttctc aggccgcaat tccccctcca
     3541 gcgaaggtga tgcgcatgga tcgccagcgg ctccgcacga cactgctgga ccttttggaa
     3601 caagagacat gggagcggcc cgagacattc accgacgatg tgaaaattcg cgaagagctg
     3661 aagctcgact cggtcgatct gttgggcgtc atgctgcggg ccgaatcgac attgggcgta
     3721 agtctcgaca gccgcgattt cgacaagatt gccacggtcg gtgacctgct ggacacgatc
     3781 cagcgcaagc tcgccgccaa accgcaaaac aaggccgcct gacgtgttca ccgcgggaca
     3841 ggacgtcgca cttgaagcgg cggtcgttgc cgctggctcg tccgccgtgc gcctgggcga
     3901 ctttgccccg ctgcgcaaag agccgccccg cttcggcggc gtcgagctgg ccgggcattt
     3961 cctcaagcat gccgacgagc agacgatcgt ggcgctaaag gcagtcgggc gggccatcga
     4021 gcggcgccgg ctcgatcccc gccaaatgac cgactgggcc atcgtcgccg ccccacgcta
     4081 catcgggcac ctggccggag cgtccattct cgaccgcttt gcccggcagg gaggcagcgg
     4141 cgtgcccccc catgccgtgg cgcaaaattc cttgcacagc atttccggcg ctgccagcat
     4201 cgtgctgggg ctgcacgggc ctaatgtcgg cgtcggcggc ggcccctggg cgatcggcga
     4261 cggcctggcc gcggccctga cgctcttcga cgccagccgc gtgccaggcg tttggctgct
     4321 cgtgacccaa ttcgagccgg aactgattcc cgacgaggca
//
LOCUS       DASUGB010000511         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_57176,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000511 DASUGB010000000
VERSION     DASUGB010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57176"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFV87_09800"
     CDS             <1..584
                     /locus_tag="VFV87_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104093.1"
                     /translation="PIRLPLIVYGHEDYGESEWSRLADVCTVRRVRSLDRLLDYAVLF
                     THRSIAALPDRQRQMLMELHQTDRVLAGRKVLIVDDDMRNIFALSTVLEEHEMRIVSA
                     DNGRDAIRILQTQPDIEIVLMDIMMPELDGLETMREIRRIPHLKDLPIVAVTAKAMKG
                     DREKCIEAGAWDYLSKPVDSEQMLAVLRAWLHR"
     gene            682..>2540
                     /locus_tag="VFV87_09805"
     CDS             682..>2540
                     /locus_tag="VFV87_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104094.1"
                     /translation="MENNKAKILIVDDLPEKLLVYRVILEDLGQELVVAQSGAEALRQ
                     VLAHDFAVILLDVNMPDMDGFETAAMIRRRKRSAHTPIIFVTAYADEVRISEGYAHGA
                     VDYILAPVVPEILRAKIKVFVDLFRMTQQVKQQAEERIALIEERSMREAAEAANRHLS
                     FLAHVGAMLGQSLDFDVTCRDAAQIAVPAIADQALVAYRSHAGGWMIHADEASREVSS
                     SETEDWESRPADWLLAAQQALASGKIEFLPNSPAAPGGPLGPETIFLPLSGRGRTFAV
                     LVLSRQQSGRVFESGDVTVAKALASRAAIALDNAALYRDIEQADRQKDEFLSMLAHEL
                     RNPLAPIRNAVAVLRARGQAQPDALWAQDVIDRQTTQLVRLVDDLLDVSRITQGKIRV
                     ELEYLSAASVVAGAVETSRPLIEAGQHRLTVDVPKEPIFIHADQARLSQVLSNLLNNA
                     AKYTPEGGSICLAVGREGDDAVFRVRDNGLGIPPDMLAQIFNLFTQVERNHDSSQGGL
                     GIGLTLVRRLVELHGGTVAAHSDGPGTGSEFVIRLPAGAAPREQPISHGNRPAIRPPH
                     VSPLRILVVDDNADAADSLARLLRLEGHLVQTASDGLAAVQTAAEFRPDAAVL"
BASE COUNT          460 a          807 c          834 g          439 t
ORIGIN      
        1 gcccgatccg gctgccgctg atcgtctatg gccacgagga ttatggcgag agcgagtggt
       61 cgcggctggc ggacgtctgc accgtgcgcc gcgtgcggtc gctggaccgg ctcctcgact
      121 atgcggtgtt gttcacccac cggagcattg ccgcgctgcc cgaccggcag cggcagatgc
      181 tgatggagct gcaccagacg gaccgcgtgc tggccggccg caaggtattg attgtcgatg
      241 acgacatgcg gaacattttc gcgctctcga cggtgctgga ggaacacgag atgcgaattg
      301 tgtcggccga caatggtcgc gacgccatcc gcattctgca aacgcagccc gacatcgaga
      361 tcgtgctgat ggacatcatg atgccggagt tggacgggct ggagacgatg cgggaaattc
      421 gccgcattcc gcacctgaag gatctgccga tcgtcgccgt caccgccaaa gcgatgaagg
      481 gcgaccgcga aaagtgcatc gaagcgggcg cctgggacta cctttccaag cccgtcgata
      541 gcgagcagat gctcgccgtg ctgcgggcct ggctgcaccg ctgaagcgga gtccaaggtc
      601 caaagtccca agtccaaagt tgggatttct ggaccttgga ccttggactt tggaccttgg
      661 actcggacac acccgttccc gatggaaaat aacaaagcca aaatcctgat cgtcgatgac
      721 ttgcccgaga agctgctggt ctatcgtgtg attcttgaag acctggggca ggagctggtc
      781 gtcgcccaat cgggcgcgga ggctttgcgg caggttctgg cgcacgattt cgccgtgatc
      841 ctgctcgacg tcaacatgcc cgacatggac ggcttcgaga ccgccgcgat gatccgccgc
      901 cgaaagcgct cggcgcatac gcccatcatt ttcgtcactg cctacgcgga cgaagtgcgt
      961 atttccgaag gctacgcaca cggcgccgtc gattacatcc tggcgccggt cgtgccggag
     1021 atcctgcggg cgaaaatcaa ggtgtttgtc gatctgtttc ggatgaccca acaggtcaag
     1081 cagcaggccg aggaacggat cgccctcatc gaggaacgca gcatgcggga agccgccgag
     1141 gcagccaacc gccacctcag ctttctcgcg catgtcggag cgatgctggg ccagtcgctg
     1201 gatttcgacg tcacctgccg cgacgccgca caaatcgcgg ttccagcgat tgccgaccag
     1261 gcgctggtgg cttaccgatc gcacgccggc ggctggatga tccatgccga cgaggcatcg
     1321 cgcgaagtgt ccagcagtga aacggaagac tgggagtcac ggccagccga ttggctgctg
     1381 gccgcccagc aagcgctggc gagcgggaaa atcgagttct tgcccaacag cccggccgcg
     1441 ccgggtgggc cgctggggcc ggaaacgatc ttcttgccgc tcagcgggcg ggggcggact
     1501 tttgccgtgc tggtcctctc gcggcagcag agcggccggg tctttgagtc gggcgatgtg
     1561 acggtggcca aggccctggc ctcgcgggcc gccatcgcgc tggacaatgc cgccctttat
     1621 cgcgacatcg aacaggcgga ccgccagaag gatgaattcc tgtcgatgct cgcccacgag
     1681 ctgcgcaatc ccttggcccc gatccgcaat gccgtcgccg ttttgcgggc ccgcggtcag
     1741 gcccagcccg acgccctctg ggcccaggac gtgatcgatc ggcagacgac gcagctcgtg
     1801 cggctggtgg acgacctgtt ggatgtctcg cgaattacgc aaggcaaaat ccgcgttgaa
     1861 ttggagtatc taagcgcggc ctcggtggtc gccggcgccg tcgaaaccag ccgcccgctg
     1921 atcgaggccg ggcagcaccg gctgacggtc gatgtgccga aagagccgat tttcattcac
     1981 gccgaccagg cgcggctgag ccaggtgctg tcgaatctgc tgaacaatgc ggcgaaatac
     2041 acgcccgaag gcggatcgat ctgccttgcc gtcgggcgcg aaggggacga cgcggtgttt
     2101 cgcgtgcgcg acaacggcct gggcattccc ccggacatgc tcgcgcagat ctttaacctg
     2161 ttcacccagg tcgagcgtaa ccacgattcg tcgcaagggg gattgggaat cggcttgacg
     2221 ctcgtccgcc gcctggtgga gctgcacggc ggcaccgtcg cagcgcatag cgacggcccc
     2281 ggcacgggga gcgaattcgt cattcgtttg ccggccggcg ccgccccccg cgaacagccg
     2341 ataagccacg gcaaccgccc cgcgattcgc ccgccgcacg tctcgccgct gcgaattctg
     2401 gtcgtggatg acaatgccga tgccgccgac agcctcgcgc gcctattgcg gctggagggg
     2461 catctcgttc agacggccag cgacgggctg gcagccgtgc aaacggccgc cgaattccgc
     2521 ccggacgccg ccgtgctcga
//
LOCUS       DASUGB010000512         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_336107, whole genome shotgun sequence.
ACCESSION   DASUGB010000512 DASUGB010000000
VERSION     DASUGB010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336107"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1245
                     /gene="mutY"
                     /locus_tag="VFV87_09810"
     CDS             64..1245
                     /gene="mutY"
                     /locus_tag="VFV87_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5104095.1"
                     /translation="MSPRSRSPDILSPARRRGLTIRLLDWFAHSARDLPWRRTSDLYA
                     IWISEIMLQQTQVATVIGYWGRFMARFPDVPSLAAADEQDVLRLWEGLGYYRRARQLH
                     AAARVIATEHGGEFPTTYEAVRSLPGIGRYTAGAILSIGLDARLPILEANTIRVLSRL
                     TAFRGDPKSAAGQKQLWSVAEAILPSNRVGAFNQGLMELGSEICTPKAPACGECPVAA
                     LCEARRRGLAEAIPRAAKRTVYEDVTEAAVVIRSGNRILLRRCQEGERWAGLWDFPRF
                     AINAIDDGNAAREVSRGVQGLLELRIASPTLLASFKHGVTRFRIALHCFEAELRADSQ
                     PIRMRRGTKAIRWVAVEQLTDYPLSVTGRKISHLLVQQVNRQIAPASAARRKCSRCNE
                     G"
     gene            complement(1181..1648)
                     /locus_tag="VFV87_09815"
     CDS             complement(1181..1648)
                     /locus_tag="VFV87_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5104096.1"
                     /translation="MSNNTFQRLTQFLDSLQVNYEVLRHAPVFTSEEAAQVRGTPLAS
                     GAKALVCKADERFVMIVLPADRKLASKDVRKSQAVKSLRFATREEVEQLTGLAPGSIP
                     PFGSLFGLPTWCDERLSENQQINFNAGDHAISISLRYTDYISAEQPTLGRFAD"
     gene            1902..3386
                     /locus_tag="VFV87_09820"
     CDS             1902..3386
                     /locus_tag="VFV87_09820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX5104097.1"
                     /translation="MSEILFQYYKIHPTTWVYVSSLLIIAVFFKFNRFWSVRNLDLVM
                     LILLAPGMLMVNLTLPWRESKTPPPTASIQSGDAAEASAAPVANPEATTLAEAMPAVA
                     PPAAAVNAMAYYGYVWLFAISALWLVRLLLDPTMVRRPLLEPNLSSGGLIFLGCSLFI
                     FLMANVITSNLSADDLRGAEGAHSLIAGRDTTGQADAPEDYRQHGPGYYLMHLLPSIP
                     STTLAGDGEGGPPLRAYMLVSKSMAILSHLAVVAALIVIGYWHFENIRMGIGAAALYL
                     MLPYTAQMTGHVDHVLPAALLLWAIVLYRRPALAGIFLGLAAGVNYYPFFLLPLWISF
                     YWQRGLARFLMGSIGAIAVLALSLVFVSKDLASFWFKFQQMFALWIPRMTDLKGIWGL
                     GWDSWYRLPVIAGCVALAAGLAIWPTHKNLGTLLSCSAAMMVAVQFWHGFGDGGGMYI
                     AWFLPLLLMTVFRPNLEDRVALTVLGEGWFLRRRARLAQLNRAA"
BASE COUNT          577 a         1167 c         1117 g          715 t
ORIGIN      
        1 ttactgaaca cggcaggtgg gccgatggct tgacgaaacg gccgcgagcg actctctggc
       61 gacatgtccc cgcggagccg atcgcccgat atcctgtcgc ccgcccggcg tcgtgggctg
      121 acaatcagac tgctcgactg gttcgcccac tcggcgcgcg atcttccctg gcgaagaacg
      181 agcgacctgt atgccatctg gatcagcgag atcatgctcc agcagacgca ggtggccacg
      241 gtcatcggct actgggggcg atttatggct cgctttccgg acgtcccctc gctggccgca
      301 gccgacgagc aggacgtact gcgactctgg gagggactgg gctactatcg gcgggcccgg
      361 caattgcatg cggcggcgcg cgtcatcgca acggagcacg gaggcgagtt tcccaccacg
      421 tacgaagcgg tccgttcgct gccgggtatc ggccggtaca ccgccggcgc gatcctgtcg
      481 atcggcctgg atgcacgcct gccgattctc gaggccaaca cgattcgtgt cctcagccgg
      541 ctcacggcgt ttcgcgggga tccgaaatcg gctgccggac agaagcaact ctggtcagtt
      601 gctgaggcga ttctgccctc gaatcgcgtg ggcgctttca atcaaggact gatggagttg
      661 gggagcgaga tctgcacgcc gaaagcgccg gcctgtgggg agtgtcccgt cgccgcgctg
      721 tgcgaggcgc gtcgccgcgg cctggcggag gcgattccgc gtgccgcgaa gcgcacggtc
      781 tatgaagacg tgacggaagc ggccgtggtg atccgctccg gcaatcgcat tctcttacga
      841 cggtgccaag agggggagcg gtgggccggg ctgtgggatt ttcccaggtt cgccatcaac
      901 gcgatcgacg acggcaatgc ggcgcgcgaa gtttctcgcg gcgtgcaggg cctgctcgag
      961 ctgcgcattg ccagccccac actcctcgcg tcgttcaagc acggcgtcac ccggttccga
     1021 attgcgctcc attgctttga agccgagctg cgtgccgatt cgcagccaat tcgcatgcgc
     1081 cgcggcacga aggccatccg ctgggtcgcc gtcgagcaat tgacagacta tccactaagc
     1141 gtcaccggca ggaagatcag ccatttgctg gtccagcagg tcaatcggca aatcgcccca
     1201 gcgtcggctg ctcggcggaa atgtagtcgg tgtaacgaag gctgatcgag attgcgtgat
     1261 cgccggcgtt gaaattgatc tgctggtttt cgctcaatcg ctcgtcgcac caggtcggca
     1321 ggccgaacag gctcccgaac ggaggaatag acccgggcgc aaggccggtg agctgctcga
     1381 cctcctcccg cgtggcaaag cgcaaactct tcaccgcttg agacttgcga acgtccttgc
     1441 tggcgagctt ccggtcggca ggcaggacaa tcatgacaaa tcgctcatcc gccttacaaa
     1501 ccagcgcctt agccccactt gcaaggggtg ttccgcggac ttgagcggct tcttcactgg
     1561 tgaaaacggg ggcgtggcga agaacttcgt agttcacctg aagggaatcg aggaactgcg
     1621 tcaatcgctg gaaagtattg ttcgacatgg ctttgcgtca attcctataa cgcggctcgt
     1681 gcccgcgacc atcaggcctt gccggcaatg gcggccgccg acgcatcctt gcaatggtgg
     1741 tgggacggcg tatcgtgtta gtttggcgcg caccgcgcaa cgggtggaaa cgccgctttc
     1801 ctgatccgct caatccgctc ccggccgcgc tccttcggtt tataacccgc ctcccagctc
     1861 aaatgggcgt ggaagtgcgg cgacgataat gctgggcgct catgagcgag atcctctttc
     1921 aatactacaa aattcatccg acgacctggg tctatgtgtc gtcgctgctc attatcgcgg
     1981 tctttttcaa gttcaaccgc ttctggagcg tccgcaacct cgacctcgtc atgctgatcc
     2041 tgctcgcgcc gggcatgctg atggtcaacc tgaccttgcc ctggcgggag tcgaagacgc
     2101 cgccgcccac ggcctcgatc caatccggcg acgcagccga agcctccgcc gcaccggtcg
     2161 ccaacccgga ggctaccacc ttggccgagg ccatgccggc cgtcgcgccg cccgcggccg
     2221 cggtcaacgc catggcttac tacggctatg tctggctgtt cgcgatttcc gccctgtggc
     2281 tggttcgcct gctgctcgat ccgacgatgg tccgccgccc gcttttggag ccgaatcttt
     2341 ccagcggcgg tttaatcttc ctgggctgtt cgctgttcat cttcttgatg gccaatgtca
     2401 tcaccagcaa tctctcggcc gacgacctgc gcggcgccga aggcgcccac tcgctcatcg
     2461 ccggccgcga taccaccggc caggccgacg cccccgaaga ctatcggcag cacggccccg
     2521 gctactactt gatgcacctt ctgccgagca ttcccagcac cacgctggcc ggcgacggcg
     2581 aaggcggccc gccgctgcgc gcctatatgc tcgtttccaa gagcatggcg attctgtcgc
     2641 acctggcggt cgtcgcggcg ttgatcgtca tcggctactg gcacttcgag aacattcgga
     2701 tgggcatcgg agcagccgcg ctgtacctga tgctgcccta caccgcccag atgaccggcc
     2761 acgtcgatca cgtgctcccc gcggctctgc tcctgtgggc gatcgtcctg taccgccgcc
     2821 cggcactggc cggcatcttt ctggggctgg cggcgggcgt gaattactac ccgttcttcc
     2881 tgctgccgct gtggatcagc ttctactggc agcggggcct ggcccgcttc ctgatgggtt
     2941 cgatcggggc gattgccgtg ctggccttgt cgctcgtgtt cgtctcgaag gacctcgcgt
     3001 cgttctggtt caagttccag cagatgttcg ccctctggat tccgcgcatg accgacctca
     3061 agggcatctg ggggctgggg tgggattcgt ggtatcgcct gccggtcatc gccggctgcg
     3121 tggctctggc ggcggggctg gccatctggc cgacgcacaa aaacctcggc acgctccttt
     3181 cctgctcggc cgcgatgatg gtcgccgtgc agttctggca cggctttggc gacggcggcg
     3241 ggatgtatat cgcctggttt ctgccgctgc tcctcatgac cgtcttccgt ccgaatctcg
     3301 aagaccgcgt cgccctcacc gtgctcggcg aaggctggtt cctccgccgc cgcgcccgcc
     3361 tcgcccaact caaccgggcg gcgtaggcaa ggtatcaatc gccgccatgg attcgtcgga
     3421 gcgcatcgcc gcagaattca atcgcctcat cgaatcgcgc cccagaacgc ctgccgagcg
     3481 ggtcgtattt gtggttgtcg tcgcgcggtg cgaaatcgac atggcgcacc tgttgtcggt
     3541 gtttgagcag gcgctggacc aggacgaagc acgggt
//
LOCUS       DASUGB010000513         6496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_475612, whole genome shotgun sequence.
ACCESSION   DASUGB010000513 DASUGB010000000
VERSION     DASUGB010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475612"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFV87_09825"
     CDS             complement(<1..235)
                     /locus_tag="VFV87_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104098.1"
                     /translation="MDTVNPYAVTPGEPAPPAIVPVAGLTVRQGIITVIGTAAAFAAI
                     GALLGTLLGLFVPSYYRTVFDAENTPGFEPAQVG"
     gene            386..1093
                     /locus_tag="VFV87_09830"
     CDS             386..1093
                     /locus_tag="VFV87_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEX5104099.1"
                     /translation="MACSFSSLTRSAAQDKPAEEQAARFRDRGEFVEDTQTGLLWQKD
                     GEASGKKNFYDAAEYAKKLKLGGLTGWRVPTAAELETIFPAKEAPFTNSGYNPDKCCG
                     GGKEFRGYWTSELDPRVEDYAFLFQWYAGGGKNNCFASKNFVFVRCVRGTLAAGETPV
                     ASTTEPAPKLDEKTIARAKALIAQLGAEQFADREKAAEELKSLGSQIAPLLREALEKA
                     TDAEVRFRLKGLLNRAE"
     gene            1289..2404
                     /locus_tag="VFV87_09835"
     CDS             1289..2404
                     /locus_tag="VFV87_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104100.1"
                     /translation="MKSTLARTWRKAGFTLVELLVVIAIIGILVALLLPAIQAAREAA
                     RRMSCSNKLHEIGLALHEYHDALNTFPPDAIYSATRKTAETTADIRMYTWLCMILPYM
                     EQKPLYDKINFGVPGYNQLISIPGHPEANASGFVALQSILLKDYICPSDPVLADLPYT
                     FAYSSYAGNAGWDPHRRKYGDSYIAGMFPLIDPQNLSDVTDGTSYVIFASEVSVEGYC
                     CYRNPEDRWIGGSGTPRRVSLGSTVTRTQLVTPCAWVGTHVWIDAPNGPGPLYYADAS
                     ATGNMWKPGWNYIQGPLHYAQHMPNQEWPAAASHHPGGHQSLLVDGSVRFIPHNISAG
                     NPAGDAYGRNGNIWAAAHYPNGIKRDGGVPKANVVWP"
     gene            2611..3708
                     /locus_tag="VFV87_09840"
     CDS             2611..3708
                     /locus_tag="VFV87_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104101.1"
                     /translation="MNRKRYGFTLVELLVVIAIIGILVALLLPAIQAAREAARRMSCS
                     NKLHEIGLALHEYHDALNTFPPDAIYSATRKTQMTANDVRMYTWLCMILPYMEQKPLY
                     DKINFGVPGYNQLISIPGHPEANASGFVALQSILLKDYICPSDPVLADLPYTFAYSSY
                     GGNAGWDSHRRKYGDSYIAGMFPLIDPQTLSDVTDGTSYVIFASETSVEGYCCYRNPE
                     DRWIGGSGSPRRVSIGSTVTRTQLITPAAWAYTHVWIDAPNGPGPLLYSDGTGPGGLW
                     HPGWNHILGPLHYAHHMPNQEWPAAGSHHPGGHQSLLVDGSVRFIPHNISAGNPAGDA
                     YGRNGFIWAAAHYPNGIKRDGGNPKANVVWP"
     gene            3851..4045
                     /locus_tag="VFV87_09845"
     CDS             3851..4045
                     /locus_tag="VFV87_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104102.1"
                     /translation="MQSKFFILLAFLAAPLCVAGCGGGTTGTYSDKSAEESVSPAAVA
                     SPPVDVSTTPATPAAPATSP"
     gene            4282..5505
                     /locus_tag="VFV87_09850"
     CDS             4282..5505
                     /locus_tag="VFV87_09850"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104103.1"
                     /translation="MGAVWFPACEATVNAEWCDRVWGDGIREIGACASILYISHGGGR
                     AMNRKCNGFTLVELLVVIAIIGILVALLLPAIQAAREAARRMSCSNKLHEIGLALHEY
                     HDALNTFPPETIWSATRKTQMTPNDVRHYTWLCLILPFMEQKPLFDKINFRAPGYNQL
                     ISIPGHPEANASGFVPLQSIMLKDYICPSDPVLADIPYGMAYTSYSGNMGWDHHRRKY
                     QDSYVAGMLPVIDPQTLSDVQDGTSYVIFVSETTTEGYCCVASADRWIGGSGSTRRAS
                     IGHSVTHTALISPAANVVSSPWVDAPAGPGPVLRADGSSGGFWWPGNHVHGPIYVAHW
                     GPNTEWPGPASHHPGGIQSLLVDGSVRFIPNSISQGNPAGDAYGRYGNIWVAAHYPNG
                     IKREAGIPKANVVWP"
     gene            5974..>6496
                     /locus_tag="VFV87_09855"
     CDS             5974..>6496
                     /locus_tag="VFV87_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5104104.1"
                     /translation="MIRTCALQFSFFFAIHAAAAAWDSHGAPNVVFVLTDDQRADCLG
                     LAGHPYLKTPNIDRLGKEGLYFKNCFCTTSLCSPSRASILSGLYAHSHGVSNNFTEYP
                     ADLPSFPRQLQAAGYQTAYIGKYHMGEENDEKRPGFDYFVTHKGQGKYWDTEFNVDGR
                     RQVLEGYYTTVVTD"
BASE COUNT         1280 a         1909 c         1882 g         1425 t
ORIGIN      
        1 acccaacctg cgcgggctca aagccgggcg tattctcagc atcgaatacc gtgcgatagt
       61 agctcggcac gaacaggccc agaagcgtgc ctaaaagtgc tccgatggca gcaaacgccg
      121 ctgccgttcc gataaccgta attatgccct ggcgcaccgt cagcccagcg actggaacga
      181 ttgcgggcgg ggcgggttct cctggcgtga cagcataggg attgactgta tccattgatg
      241 ggctcggggc acaaacctca aagcaattgc atctaccccg ctttgtcgct ctggaattcg
      301 gacactggca atggtaacaa acgggtagct cgggaggtgg atgatgtgca gatttctgac
      361 cggtgcctgt ttcctcgcaa tcgcagtggc ttgctcgttc tcgtcgctga cgcgctccgc
      421 ggctcaggac aagcccgcgg aagaacaggc ggcgcgcttc cgcgaccgcg gcgaattcgt
      481 cgaagacacc caaacgggcc tcctctggca gaaggacggc gaagcctccg gcaagaagaa
      541 cttctatgac gcggccgagt acgccaagaa gctgaagctc ggcggcctca caggctggcg
      601 ggtgcctacc gcggcggagc tggagacgat ctttccggcc aaggaagcgc cgttcaccaa
      661 ttccggctat aaccccgata agtgctgcgg cggcggcaag gagttccgcg gctactggac
      721 gagcgagctc gatccgcggg tcgaggacta tgcttttctc tttcagtggt acgcgggtgg
      781 cggcaagaac aactgcttcg ccagcaagaa ctttgtcttt gtccgctgcg ttcgcggaac
      841 actggctgcc ggcgaaacgc ccgtcgcctc gacgacggag cctgctccca agctcgacga
      901 aaagacgatc gctcgcgcca aggcgctcat cgcccagctt ggcgccgaac agtttgctga
      961 ccgcgaaaag gccgccgaag agctcaaatc gctcggttcg cagatcgccc cgctgttgcg
     1021 tgaggcgctg gaaaaggcaa ccgatgccga ggtgcggttt cgtttgaagg gcctcttgaa
     1081 ccgggcggag tgaccgccac ccagtccgcc gatctgtgca actctttcgg gtgatactgg
     1141 cgacaatcca gcacagacct gcaatttaag caaaaaaaac aaaaaaactt gcgtaggggg
     1201 gccgaaaggg ctaacatgct tctggttgtg atccgccgat tggcggcacg atcgttattt
     1261 tcctgttctt catttcagga gtcttcgtat gaagtccacc ttggctagaa cctggcgaaa
     1321 agccgggttc acgctcgtgg agctgttggt ggtgattgcc atcatcggca tcctcgtagc
     1381 gctcttgttg cccgcgatcc aggcggcgcg tgaagctgcc cggcgcatgt cgtgcagcaa
     1441 caagttgcac gaaatcggct tggcgttgca cgagtatcac gacgccttga acacgttccc
     1501 tcccgacgcg atctattctg cgacccgcaa gacggcggag accaccgccg acatacggat
     1561 gtacacgtgg ctgtgcatga ttctccccta catggagcag aagccgctgt acgacaagat
     1621 caactttggc gtgcctggct acaaccaact catctccatt cccggacatc ccgaagcgaa
     1681 cgcttcggga ttcgtggcgt tgcagtcgat tctgttgaag gactacattt gcccttctga
     1741 tccggttctc gccgatcttc cctatacgtt cgcgtacagc tcttatgctg gaaacgcggg
     1801 atgggatcct caccgtcgca agtacggcga ttcgtacatt gcagggatgt ttccgctcat
     1861 cgaccctcag aatttgagtg acgtgaccga tgggacgtcg tacgtcattt tcgcgagcga
     1921 agtgtcggtg gaaggttatt gctgctacag aaatcccgaa gatcgctgga tcggcggaag
     1981 cggcacgccg cgcagagtga gcctcggatc aacggtaact cgcactcagt tggttacgcc
     2041 ttgtgcctgg gttggtaccc acgtctggat cgacgcgccg aatgggcctg gtccgctcta
     2101 ttacgctgat gcgagtgcca ctgggaacat gtggaaaccg gggtggaact acattcaagg
     2161 gcctctccac tatgcccagc acatgccaaa ccaagaatgg ccggcagcgg caagtcatca
     2221 tccaggtgga catcagtcgt tgctggttga cggttcggtg cggttcatcc cgcataacat
     2281 ttcggcggga aatccagcgg gagatgccta cgggcggaat ggaaacatct gggccgccgc
     2341 ccactatcca aacggcatca agagagacgg cggcgttccc aaagccaacg ttgtttggcc
     2401 gtaacctgtg ctgatcggac gatcgacaca tcttggaggc ggcggattct ggccgccgcc
     2461 tctaatcttc acagcggcgc tctgggataa tctggacgtg tgacgccacg gtcagcgcta
     2521 acggtcgcgg ctggcttcga ggcgatggcg tccgagaaac cggggctggc acttttactt
     2581 ctcttcattg gtcatggtgg agggcgagcg atgaatcgca aacgctacgg gtttacgcta
     2641 gtggaattgt tggtcgtgat cgccatcatt ggcattctcg tggcgctatt gctgcccgcg
     2701 atccaggcgg cgcgtgaagc tgcccggcgc atgtcgtgca gcaacaagtt gcacgaaatc
     2761 ggcttggcgt tgcacgagta tcacgacgcc ttgaacacgt tccctcccga cgcgatctat
     2821 tccgcaaccc gcaagacgca gatgactgcg aatgatgtac gtatgtacac ttggctgtgc
     2881 atgatcctcc cctacatgga gcagaagcct ctgtacgaca aaatcaactt tggcgtgcct
     2941 ggctacaacc aactcatctc cattcccggt catcctgaag cgaacgcttc gggattcgtg
     3001 gcgttgcagt cgattctgtt gaaggactac atctgccctt ctgatccggt tctcgccgat
     3061 cttccctata cgttcgcgta cagctcctac ggcggaaatg cgggttggga ctctcaccgt
     3121 cgcaagtacg gcgattcgta cattgcaggg atgtttccgc tcatcgaccc tcagacgttg
     3181 tctgacgtga cagacggcac ctcctatgtg attttcgcaa gtgaaaccag tgtggaagga
     3241 tattgctgct acaggaatcc ggaagaccgg tggatcggcg gaagcggctc accgcggagg
     3301 gtatcaatcg gttcgacggt gacgcgcacc cagttaatta cgcctgccgc ttgggcctat
     3361 acccacgttt ggatcgatgc gcccaatggt cctggaccac tgctgtactc ggatggaact
     3421 ggcccgggtg gcttgtggca tcctggctgg aaccacattc ttgggcctct tcactatgct
     3481 caccacatgc caaatcaaga atggccggca gcggggagtc atcatccggg cggacatcag
     3541 tcgttgctcg ttgacggctc ggtgcgattc attccgcaca acatttcggc gggaaatccg
     3601 gcgggcgacg catacgggcg caatggcttc atttgggcag ccgcccatta cccgaacggc
     3661 atcaagagag atggcggcaa tcccaaagcc aatgtcgttt ggccgtaacg tgtgctgatt
     3721 ggacgatgga ttcatcttgg aggcggcgga ttctggccgc tgcctctttt ctttccggcc
     3781 gcgtcctggg acaatctgga cgtgtgaaac ccttggagag tccgaaagcc ctggtctcgg
     3841 agtaattcaa atgcagtcca aattctttat tctcttggca ttccttgccg ctcctttgtg
     3901 tgttgccggc tgcggcggcg gtacgacggg tacctactcg gacaagtcag ccgaggaatc
     3961 ggtttccccc gcagccgtcg ccagtccgcc ggtggatgtt tccacaactc ccgcaactcc
     4021 cgctgctccc gccaccagtc cctaaacaag cggctgggca gatttctacg ctttgtggtg
     4081 ccgctgcgcg cgcgctgtcc cttgtcagcg agcggcccct ctggaagagc taggttgctc
     4141 taaatttcct gaatttattc caaaatctgg ttgtattgtc cgccggatga cgttagcgta
     4201 acgtctgccg gctggatcgc ttggggttaa ttttcttaac tgcgattctc gctggcattc
     4261 ccggcaattc tgacccggga cgtgggtgcc gtctggttcc cagcgtgtga agccacggtc
     4321 aacgccgagt ggtgcgaccg ggtttggggc gatggcatcc gagaaatcgg ggcttgtgct
     4381 tctattctct acattagtca tgggggaggg cgcgcgatga atcgcaaatg caacggtttt
     4441 actctggtgg aattgttggt ggtgatcgcc atcattggaa tcctcgtggc gcttttgttg
     4501 cctgcgatcc aggcggcccg agaagccgct cggcgcatgt cgtgcagcaa caagctgcac
     4561 gagattggtt tggccctgca cgaataccat gacgcgctga acacgttccc tcccgagacg
     4621 atttggtcgg caacgcgcaa aacgcagatg acgcccaatg acgtgaggca ctacacctgg
     4681 ctgtgcctga tcttgccatt catggagcag aagcccctgt tcgacaagat caactttcgc
     4741 gcaccgggct acaatcagct gatttcgatc ccgggccatc cggaggcgaa tgcctcgggc
     4801 ttcgtccccc tgcagtcgat catgctgaag gattacatct gtccgtccga ccccgtgctg
     4861 gcggatattc cctacggcat ggcctatacg tcctactcgg gaaacatggg ctgggaccat
     4921 caccgccgca aatatcagga ctcctacgtc gcaggcatgc ttcccgtgat tgatccgcaa
     4981 accttgtcgg atgtgcaaga cggcacgtcg tatgtgatat ttgtcagcga gacgaccacc
     5041 gagggatatt gctgtgttgc ttctgccgac cggtggatcg gtggatccgg atccactcgg
     5101 cgggcctcca tcggacattc ggtcacgcac acggcgctga tctctcccgc tgccaacgtc
     5161 gtcagctcgc cgtgggtcga tgcgcctgcc ggtcccgggc cagttctacg tgccgacgga
     5221 agctctggtg gtttttggtg gccgggtaac cacgtccatg ggcccatcta cgttgctcac
     5281 tggggtccga acacggaatg gccgggacct gcaagccacc atccgggcgg aattcagtcg
     5341 ctgctcgtgg acggctcggt tcgattcatt cctaattcga tttcgcaggg gaacccggcg
     5401 ggcgacgcct atggtcgcta cggaaatatc tgggttgctg cccactatcc gaacggcatc
     5461 aagagggaag ccggaattcc caaggcaaac gtcgtctggc cataacaata gattcccaat
     5521 gatccggctc aacgtgatct cgtgaatggc ggtggatagg tccttcgcca tgtgtcgctt
     5581 cgtgtactca tcgtgcctgg ccccgcgcca gcgatttccc tttccctgga gtgcgttccc
     5641 atgcaacgag tcatcgttgg cacagtgttg acattggcta gtccgttttt attcgccggc
     5701 tgcggtacgg ggagcacagg catatactcg gatgacgcag cccaggaatc cgtgtcccct
     5761 gcggcgacaa attaccttca gtcgcctgag aacccggcag caatgccggc tgccgatgct
     5821 tctggcgcac cgggcgctgc acccgcccgc tgatcgaggt tcgcgatcgt tcgggccagg
     5881 gtagaattag ctcgtcgttt tgccgatcgc ttcgcacggt ggaatctgcg gcgctagtcg
     5941 cttctaccac ccacgacgaa tatcctaagt tccatgattc gcacttgtgc attgcagttc
     6001 tcgttctttt tcgcaattca tgccgcggcc gccgcttggg attcgcatgg agctcccaac
     6061 gtcgttttcg tcctcaccga cgaccagcgg gccgattgcc tcggcctggc gggccatcct
     6121 tatctcaaaa cgccgaacat cgaccggctg ggcaaagagg ggctctattt caagaattgc
     6181 ttctgcacca cttcgctctg ttcccccagc cgggcgtcca tcttgagcgg cctgtatgcc
     6241 cattcgcacg gcgtctccaa caacttcacc gagtatcccg ccgatttgcc cagctttccg
     6301 cggcagcttc aggccgccgg ctaccagacg gcttacatcg gcaagtacca catgggagag
     6361 gagaacgacg aaaaacgtcc cggcttcgat tatttcgtga cccacaaagg ccaggggaag
     6421 tattgggata cggaattcaa cgtcgatggc cggcggcaag tgctggaagg gtactacaca
     6481 accgtcgtca ccgacc
//
LOCUS       DASUGB010000514         3963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_752224, whole genome shotgun sequence.
ACCESSION   DASUGB010000514 DASUGB010000000
VERSION     DASUGB010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752224"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFV87_09860"
     CDS             complement(<1..566)
                     /locus_tag="VFV87_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104105.1"
                     /translation="MTLVGKIFTVLIFVMSIVFMSFAVMVFATHKNWKLYADNASPAP
                     GQKLGLKQQYEQLQQLKKQADDQLNQLKNELAEEQAARKQALAALQSQLTQAQTKLAA
                     QQAEYDRLLADNTTATQTAKDAQARLASLEAENLARREALRTVMKDLDTKFAEVVALT
                     DQLNQAESLKAILQERNKEAADQMAKMKL"
     gene            complement(955..1302)
                     /locus_tag="VFV87_09865"
     CDS             complement(955..1302)
                     /locus_tag="VFV87_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104106.1"
                     /translation="MNLPFIDFGQQTKQAKDEPSPAALGTSIPNAPFVIRFEGRKAAG
                     QKHFKVLVYLKKRRAQGDLSIIASCRVEPIGDPNADVAIYEGTCRKPALKGDCLIEVK
                     RGKAYLVRSTEVR"
     gene            complement(1912..2823)
                     /locus_tag="VFV87_09870"
     CDS             complement(1912..2823)
                     /locus_tag="VFV87_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104107.1"
                     /translation="MRRAALTLVEVLVVLAIIGLLIALLLPAIQSARESGRRIKCSTN
                     MRQIALALHQYHDVHGVLPAYTCANYSFHVSLLPHLEQEPLFRQFNFGINAMDYQGDL
                     ASTRVSVLECPSDGSRGSTADGRFLAPTNYFGNSGSGAQRYGFNGVFAYGEHPLGSID
                     RLPLAGITDGLSQTAMLAEAATGDRTADLRRCVWDIPGSEPPDQFEAFVAFCRQAPNT
                     APQFGLSRGRPWFEVSLCVKLYNHVLPPNGPNCMSVPFWKHRISTSSSMHKSIVNVAM
                     ADGSVHAVNDDVDVTVWRKLGSRNGTP"
     gene            complement(2893..>3963)
                     /gene="lnt"
                     /locus_tag="VFV87_09875"
     CDS             complement(2893..>3963)
                     /gene="lnt"
                     /locus_tag="VFV87_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5104108.1"
                     /translation="LAATLGYGYWRLNQTPPGAAGPVAKIALIQGSIDTVFEPPTSEK
                     VRATYDHYNTLMADAVTEQPNLDLVVWPESMFVIPERMIEEPLVVPADAGISTDELRS
                     GIAAASSEFEMVLAAEAARANENSESTGPGTHLLVGTNTFIYGQNQPRSYNTALLANR
                     HGKVAGRYYKTHAVMFGEYIPFGDVLPWIYRLTPMPGGMSVGEGPEAFEIAGLRMSPS
                     ICFESTVPHLIRGQVIELARRDQPVDVLVNVTNDGWFWGSGILDQHLRCGVLRAVENR
                     KPLVVAANTGISAHIDGNGVIRQRGPKRDRQAIVAQVQADGRISPYHWLGDLPANVCA
                     LACLGLALVGSLNWNSSTNQAS"
BASE COUNT          754 a         1175 c         1231 g          803 t
ORIGIN      
        1 agcttcatct tcgccatctg atcggcggct tccttgttgc gttcctgcaa gatcgccttg
       61 aggctctcgg cctgattgag ctggtctgtc agagcgacca cctcggcaaa cttggtatcc
      121 agatctttca ttaccgttcg cagcgcttcg cggcgggcca gattttcggc ttccagggag
      181 gccaggcggg cctgggcgtc cttggcggtt tgcgtggcgg tggtgttgtc ggccagcagg
      241 cggtcgtatt cggcttgctg ggccgcaagc ttcgtctggg cctgggtcaa ctggctctgc
      301 agggcggcca gcgcctgctt gcgggcggct tgctcctcgg ccaactcgtt cttgagctgg
      361 ttgagctggt cgtcggcctg ctttttgagc tgctgaagct gctcgtattg ctgcttgagc
      421 cccagcttct ggccgggggc cgggctggcg ttgtcggcgt acagcttcca gttcttgtgc
      481 gtcgcgaaca ccatcaccgc gaacgacatg aagacaatgc tcatcacgaa aatgagcacg
      541 gtgaagattt tgccgacgag tgtcatggca ggcagtcccc tggttcaggc tgggcaacgt
      601 aggacgggtt tccaacccgt tcgaggcttt cgagttcttc acgcggacgg cttggaaagc
      661 cgtcccacgg cgcggcagtt ccgccgaggc gaagggagcg ataagtccgg accgaaatac
      721 ccccgcagta taattacggg ccaaatgaaa agtcaacgaa acgggcaatt tcgggcattt
      781 ccggccttta cgcaggctgg cgaaggcagc cggcggggcc gaactgtatg gaagattcga
      841 tcctgcctgc tggcattatt ccgctctctc gttcacaccc gagcggtact aacccgcacg
      901 gcgagaattt ggcgccgaaa ttcgcacttg agatcgacaa ctcgcccttg cggcctacct
      961 cacttcagtc gagcggacga ggtacgcctt gccgcgtttc acttcgatca ggcaatcccc
     1021 tttgagtgcc ggttttctgc aagttccttc atagattgcc acatcggcgt tgggatcgcc
     1081 aatcggctcg acgcgacaac ttgcaatgat ggacaggtcg ccctgggcgc gccgcttctt
     1141 gagatagacg aggaccttga agtgcttctg gcccgcggct ttccgccctt cgaagcgaat
     1201 gacaaatggg gcgttgggaa tggatgttcc caacgcggcc ggcgagggtt cgtccttggc
     1261 ctgttttgtc tgttgtccaa aatcgatgaa tggaaggttc acgcgaggca acgccggatc
     1321 cggtctttcc acgatttcgg atttcgcttc gagtgccgtg tccggggcac cgcaaccggc
     1381 acaacaggcc agggcgacgg cgatccatgc ggtccagtcc ggttgtcggc gtttgcggca
     1441 gcgccaggag tgggaaaaca tcgtacgact cgctcacatt tcgtcggctc tcacaatgaa
     1501 ctggacctcg tctttcgtca tgcccccagc gattagttta agtttgtagc tgccctccgg
     1561 gtacgacgtg tgttgggggg gagggcggct tcgcggtgca gtcccagtga cccgtctcat
     1621 cggtagcgat ggtgtgacca tctgaagtat ccatgaaccc ccacgattcc caaacgctgc
     1681 cagtgtacct gaactgatac aaatctgctg acgattcctc gggtccgtca ccatcacacg
     1741 gaacctcgag gaccggctgt gtcatccccg agccatcggg cgaagtaatt gcccacacca
     1801 acgccagcgc aggcgaagaa gcgcttagca ctatcaaccc tgcggccagg aaacggaatc
     1861 gcaaatcggc aatcattttt ggacctcctt acgtcaaaaa agagagaaac cctacggagt
     1921 gccgttgcgc gagccgagct tgcgccagac agtcacgtca acgtcatcat tgacggcatg
     1981 aacgctcccg tcagccatcg ctacgttgac gatcgatttg tgcatgctcg aactcgtgga
     2041 aatgcggtgc ttccagaagg gaacactcat acaattcggc ccatttggcg gcaggacgtg
     2101 gttgtacagc ttgacgcaca ggcttacttc aaaccaaggc ctcccgcgac ttaacccaaa
     2161 ctgcggcgcc gtgtttggcg cctgccggca gaaggccacg aaggcttcaa attggtctgg
     2221 cggttcgctg cctgggatat cccagacgca acgccgtagg tccgctgtac gatcgcccgt
     2281 tgccgcttcc gcgagcatcg ctgtctgact caggccgtcc gtaatgccag ccagcggaag
     2341 tcggtcaatc gatccgaggg gatgctcgcc atacgcaaag accccgttga agccgtagcg
     2401 ttgcgctccg ctgccggagt ttccaaaata gttcgtcggc gccagaaaac gcccatcggc
     2461 ggtcgaaccc cggctgccgt cgctggggca ctcaagtacc gacacccgcg tagaagcgag
     2521 atcgccttgg taatccatcg cgttgatgcc gaagttgaac tggcggaaca acggttcctg
     2581 ctccagatgc ggcagcaacg agacgtgaaa tgaatagttg gcgcaagtgt aagccggcaa
     2641 aacgccatgc acgtcatgat actggtggag cgccagagcg atctggcgca tattggtcga
     2701 acacttgatg cggcggcccg actcgcgcgc ggattgaatg gcgggcaaca agagggcgat
     2761 caataagccg atgatcgcca ggacgaccag aacttcaacg agcgtcaggg ctgcgcgacg
     2821 catcgcccca cccccccaaa gagatctcgt tagtctaggg gggggcgctg ttgattgtca
     2881 atacgccgcg tttcaagaag cttggttggt ggatgaattc caatttaacg atcccaccag
     2941 cgccagcccc aggcatgcga gggcgcatac gttggcgggg aggtcgccga gccagtggta
     3001 ggggctgatg cggccgtcgg cctggacttg ggcgacgatc gcctgccggt cgcgcttggg
     3061 gccgcgctgg cggatgacgc cattgccgtc gatgtgggct gaaatgcccg tattggcggc
     3121 aacgaccagg ggcttgcggt tctcgaccgc tcgcagtacg ccgcaccgca ggtgctggtc
     3181 gagaatgccc gaaccccaga accagccgtc gttggtcacg tttaccagca catcgaccgg
     3241 ctggtcgcgg cgggccagct cgattacttg gccgcggatc aagtgcggga cggtgctctc
     3301 gaagcagatg ctggggctca tccgcaggcc ggcgatttcg aacgcctccg gcccctcgcc
     3361 gaccgacatg ccgccgggca tcggcgtgag gcgatagatc cacggcagca cgtcgccgaa
     3421 gggaatgtac tcaccgaaca tcacggcgtg cgtcttgtaa tagcggccgg cgaccttgcc
     3481 gtggcggttg gccagcaacg ccgtattata cgaccgcggc tgattctggc cgtagatgaa
     3541 cgtattggtg ccgacgagca ggtgcgtgcc cgggcctgtg gactcgctat tctcgttcgc
     3601 gcgggcggct tcagcggcca ggaccatctc aaactcgctg ctggctgcgg cgatacccga
     3661 ccgcagctcg tcggtggaaa tgccggcatc ggcaggaacg acgagcggct cttcgatcat
     3721 ccgctccggg atcacgaaca tcgattcggg ccagaccacc agatcgagat tgggctgttc
     3781 ggtgaccgcg tcggccatga gcgtgttgta atggtcgtag gtcgcacgga ccttttcgga
     3841 tgttggcggc tcgaagacgg tgtcgatcga gccttggatc agggcgatct tggccaccgg
     3901 accagcggcg ccgggcggcg tctggttcag ccgccaatag ccgtagccaa gtgtggccgc
     3961 aag
//
LOCUS       DASUGB010000515         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_197529, whole genome shotgun sequence.
ACCESSION   DASUGB010000515 DASUGB010000000
VERSION     DASUGB010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197529"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFV87_09880"
     CDS             complement(<1..964)
                     /locus_tag="VFV87_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104109.1"
                     /translation="MCSALLACLALAPLARAEEPPRKWLKATAYAVPKETAPEGEGYF
                     SIVTGLDGKLYIGTHANAVNAWLVEFDPKAKQMKTVVDCHKAIGIDLKGFGSQAKIHT
                     RNNVGASGKIYFGTKQGYPSKDEKREDYPGGYPMVYDPQTGETKVYPIPVPHHGINSI
                     TPDESRGVAYISTCSDHRPGPGENAIFLILDLKTGKYRELMDTEHFYGFIVVDYKGRA
                     YHPILGGDIARYDPSTDKLERLKQSIDGQPPSDETRLALSPKPDPINWDISPDGKTLY
                     SVPMSGNGLYSYDLTAEGDTLPGKSHGILIAGAKSTDCRAMCVGK"
     gene            complement(1045..2739)
                     /locus_tag="VFV87_09885"
     CDS             complement(1045..2739)
                     /locus_tag="VFV87_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5104110.1"
                     /translation="MKTQLLSAVLFVAGVSATMAQSPLVFRDAGDEAGLFPDVAKIAG
                     HGVGWGDVDGDGWADLYVGAFGGHPYDSKPNQFFRNDMGKFRLDDQPQLRVLGRANGA
                     IFIDLDNDGDLDLYTTNHAIDGGAAQPHYAEPNHLFRNDGGGKFTDVSNESATCPAGI
                     AARSVTATDYDGDGLLDLFVGECFFQGGQSRTRLYRNLGGLKFENVSQAVGLPVELTG
                     FGTVAGDVNGDGWPDLFVGGRHHGNKLFINDGKGKFTELPISHGDFTWSYKDTPDDTA
                     CGVHIADANRDGRADILVGSHCDRPWFTGGVPVRLWINRGNDGNLPKFEDVTEKAGLV
                     PLPMKSPHVEIQDFDNDGWPDIYTSIVKFAANGQPHPVIFRGLGVNGGVPSFKTDALA
                     VNDFPTDEEKKMGNVGPFFEKMQKEHRIVYMAPGPSGDYNRDGKLDLFLANWWVDDRS
                     LLLKNETAGGHWLDVAVTAPAADGGQSPPYNRQGIGAVVRIYAPGKLGQAAALLGARE
                     IAVGYGYASGQEAIAHFGLGAGEACDIEIILPHGKGKLERKDVKANQRISIPGDNS"
     gene            2831..>3028
                     /locus_tag="VFV87_09890"
     CDS             2831..>3028
                     /locus_tag="VFV87_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104111.1"
                     /translation="MTQPADSLLADTPEPPYYAVIFTSLRTPADPEGYAVTADRMVEL
                     ARQQPGFLGVESARGAEGLGIT"
BASE COUNT          500 a          969 c          968 g          591 t
ORIGIN      
        1 atttgccgac gcacatcgcc cggcagtcgg tcgatttcgc cccggcaatc aggatgccgt
       61 gcgattttcc cggcagtgta tcgccctccg ccgtcaggtc gtacgaatac agaccgttgc
      121 cgctcatcgg caccgagtag agcgtcttcc cgtcgggcga aatgtcccag ttgatcgggt
      181 ccggcttcgg cgagagcgcc agccgcgttt cgtcggaggg cggctggccg tcgatcgatt
      241 gcttgagccg ctccagtttg tcagtgcttg ggtcgtagcg ggcgatgtcg ccgccgagaa
      301 tcggatggta cgcccgccct ttgtaatcga cgacgatgaa gccgtagaaa tgctcggtgt
      361 ccatcagctc gcggtacttg ccggtcttca ggtcgagaat caggaagatc gcgttctcgc
      421 cggggccggg gcggtggtcg gagcaggttg agatgtacgc cacgccgcgc gactcgtcgg
      481 gcgtgatact gttgatgccg tggtgcggca caggaatcgg atagaccttc gtctcgccgg
      541 tctgcgggtc atagaccatc gggtagccgc caggatagtc ctcgcgcttc tcgtccttgc
      601 tcggatagcc ctgcttggtg ccgaaataga tcttgccgct ggcgccgacg ttgttgcggg
      661 tgtggatttt cgcctgcgag ccaaaaccct tgaggtcgat gccgatcgcc ttgtggcaat
      721 cgacaacggt cttcatctgc ttggccttgg gatcgaactc gaccagccat gcgttgaccg
      781 cgttggcatg cgtgccgatg tacagcttgc catcgagccc cgtgacgatc gagaaatagc
      841 cctccccttc cggcgcggtt tccttcggca cggcgtaggc ggttgccttg agccacttgc
      901 gcggcggctc ttcggcacgg gccagcggcg caagggcgag acaggcgagg agggccgagc
      961 acactaatcc gacgcgccag cgagggcgtg acgagtggac tcccccgctt acgcgtcggg
     1021 ttggcgtcga tgggatgtat cgcgtcatga attatctcct gggatcgaga tacgctgatt
     1081 cgccttcacg tccttccgct ccagcttccc cttgccgtgg ggcaggatga tttcgatgtc
     1141 gcaggcttcg cccgcaccga ggccgaagtg ggcgatggct tcctggccgg aagcgtagcc
     1201 gtagccgacg gcgatttcgc gcgcgccgag gagcgcagcg gcctggccga gcttgccggg
     1261 ggcatagatg cggacgaccg cgccaatgcc ctgccgattg tagggtgggc tctgcccacc
     1321 atccgccgcc ggcgcggtca ctgccacatc gagccaatgc ccgcctgccg tttcgttctt
     1381 gaggagcagc gatcggtcat cgacccacca gttggcgagg aacaggtcga gcttgccgtc
     1441 gcggttgtag tcgccactgg ggccgggggc catatagacg atccggtgct ccttttgcat
     1501 cttttcgaag aacgggccga cgttgcccat cttcttctct tcgtcggtcg ggaagtcgtt
     1561 cacggcgagg gcgtcggtct tgaaactcgg cacgccgcca ttcacgccca ggccgcggaa
     1621 gatcaccggg tgcggctgcc cattggcggc gaacttgacg atgctcgtat agatgtccgg
     1681 ccagccgtca ttgtcgaaat cctggatctc gacgtgcggg ctcttcatcg gcagcggcac
     1741 gaggcctgcc ttttccgtca cgtcctcgaa ctttggcaag ttgccgtcgt tgccgcgatt
     1801 gatccacagc cgcaccggca cgccgccggt gaaccagggg cgatcgcagt gcgagccgac
     1861 gaggatatcg gccctgccgt cgcgattcgc gtcggcaatg tgtacgccgc aagccgtatc
     1921 gtccggcgtg tctttgtagc tccaggtgaa gtcgccatgc gagatcggca gctcggtgaa
     1981 cttccccttg ccatcgttga tgaacagctt gttcccgtga tgccggccgc cgacgaagag
     2041 gtccggccag ccgtcgccgt tcacgtcgcc tgccaccgtg ccaaagccgg tcagttccac
     2101 cggcaggccc accgcctggc tgacgttctc gaacttcagc ccgcccagat tgcgatagag
     2161 ccgcgtgcgg ctctggccgc cttgaaaaaa gcattcgccg acgaagaggt ccaagagccc
     2221 atcgccgtca tagtcggtcg cggtcacgct gcgggcggcg attcccgcgg ggcaggttgc
     2281 cgattcgttc gagacgtcgg tgaatttgcc gccgccgtcg ttgcggaaca ggtggttcgg
     2341 ctcggcatag tgcggctgtg ccgcgccacc gtcgatcgcg tggttggtcg tgtagagatc
     2401 caggtcgccg tcgttatcca gatcgataaa aatcgccccg ttcgcccggc cgagcacgcg
     2461 aagctgcggc tgatcatcga gccggaattt gcccatgtca ttgcggaaaa actggttggg
     2521 cttggagtca tacggatggc cgccgaacgc gccgacgtac aaatcggccc agccgtcgcc
     2581 atccacgtcg ccccagccca cgccatgccc ggcgatcttc gccacatcgg gaaacagccc
     2641 ggcctcgtcc cccgcatcgc gaaagacgag tggcgattga gccatggtcg cggagacgcc
     2701 ggcaacaaac agaacggccg aaagcagttg cgtcttcatc ccaagctcct cgatccgata
     2761 cgaagggtca cgagcatgat aggatgcccg cagcggcagg tgcaatcagc atggccgccc
     2821 gcgattagct atgacgcaac ctgccgactc gttactcgcc gacacgcccg agccgccgta
     2881 ttacgcggtg attttcacgt cgcttcgcac gccggctgat ccggagggct atgccgtcac
     2941 cgccgaccgc atggtcgagc tggcccgcca gcagcccggc tttctcggcg tcgaaagcgc
     3001 ccgcggcgcg gagggcctag gcatcacc
//
LOCUS       DASUGB010000516         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_197686, whole genome shotgun sequence.
ACCESSION   DASUGB010000516 DASUGB010000000
VERSION     DASUGB010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197686"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /gene="pgi"
                     /locus_tag="VFV87_09895"
     CDS             <1..1432
                     /gene="pgi"
                     /locus_tag="VFV87_09895"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5104112.1"
                     /translation="LASECGLRERTTAMFRGERINATEDRAVLHVALRAPRGASIVVD
                     GQNVVPQVHEVLDKLAAFASRARSGQWLGHTGRRIKNVVNIGIGGSDLGPVMAYEALR
                     HYSNRGLTFRFVSNVDGTDFAEAVVDLDPAETLFIVASKTFTTQETMANAHAARAWTA
                     AALGSGKEVTAKHFVAVSTNAEAVAEFGIDTANMFGFWDWVGGRYSMDSAIGLSTMIA
                     IGPEQFRELLAGFHAMDEHFRTASFERNLPVLMGLLAVWYSDFLGAGTIAVLPYDQYL
                     KRFPAYLQQLTMESNGKSVTLDGSRVGYETGPIYWGEPGTNGQHSFYQLIHQGTKLIP
                     CDFVAFCQSLNPVGNHHDLLMSNVFAQAEALAFGKTAAEVAAEKTPAWLVPHRTFEGN
                     RPSNTLLLERLTPAALGKLVALYEHSVFTQSVIWNINAFDQWGVELGKKLAQRIIPEL
                     ESAAEPNLAHDSSTNTLIRRYRSQKK"
     gene            complement(1418..1921)
                     /locus_tag="VFV87_09900"
     CDS             complement(1418..1921)
                     /locus_tag="VFV87_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104113.1"
                     /translation="MRFRLTTDWRWLQILLFTAGFAGWAAVWGIVSTRNRRYRISILT
                     SALGECPPTELKLCWGLMFLGGCAALTIPISLLFLWGPFLFPTMYFALLVGVMAVARG
                     AARDTQGLGHVATLQSICFVACDPINLILGAMELNLLRRPSVQEFLRHANGGQADEQR
                     KRWLFFL"
BASE COUNT          493 a          794 c          747 g          396 t
ORIGIN      
        1 gctcgccagc gagtgcggcc tgcgcgagcg gaccaccgca atgttccgcg gcgagcggat
       61 caacgcgacg gaggatcggg cggtcctgca tgtcgccctg cgggcgccgc gcggggcgtc
      121 gatcgtggtg gatggacaga acgtcgtccc gcaggtccac gaagtgctgg acaagctggc
      181 cgccttcgcc agccgcgccc gcagcggcca gtggctgggc cacaccggcc ggcggatcaa
      241 gaacgtcgtc aacatcggca tcggcggctc ggacctgggg ccggtgatgg cttacgaggc
      301 cctgcggcac tacagcaacc gcggcctgac attccgcttc gtctcgaacg tcgatgggac
      361 cgacttcgct gaagcggtcg tcgatctcga cccggctgaa acgctgttca tcgtcgcttc
      421 caaaacgttc acgacgcagg agacgatggc caacgcccac gcggcccgcg cgtggacagc
      481 cgccgcgctc ggcagcggca aggaagtgac cgcgaagcac ttcgtcgccg tctcgacgaa
      541 tgccgaggcg gtcgccgagt tcgggatcga cacggccaac atgttcggct tttgggactg
      601 ggttggcggg aggtattcga tggattcggc gatcggactc tcgacgatga tcgccatcgg
      661 ccccgagcag tttcgcgaac tgctcgccgg ctttcacgcc atggatgagc acttccgcac
      721 ggcctctttc gagcggaatc tgcccgtgct gatgggcctc ttagccgtct ggtacagcga
      781 tttcttaggt gcgggaacga tcgccgtgct cccctacgac cagtatctca agcggttccc
      841 cgcctacctg cagcagctca cgatggagag caacggcaaa tcggtcacgc tggacggcag
      901 ccgcgtcggt tacgaaaccg ggccgatcta ctggggcgag ccgggaacga acggccagca
      961 ctcgttctat cagctcatcc accaggggac gaagctgatt ccgtgcgatt ttgtggcgtt
     1021 ctgccagtcg ctgaacccgg tcgggaacca ccacgatctg ttgatgtcca acgttttcgc
     1081 ccaggccgaa gcgctggcct tcggcaagac cgcagccgag gtcgccgccg agaagacgcc
     1141 ggcctggctg gtgccgcacc gcacgttcga ggggaaccgg ccatcgaata cgctgctgct
     1201 ggagcggctg acgcctgcgg cgctcggcaa gctcgtcgcc ttgtacgagc acagcgtatt
     1261 cacgcagagc gtcatctgga acatcaacgc cttcgaccag tggggggtcg agctgggcaa
     1321 gaagctcgcc cagcggatca tccccgaact ggagtccgcc gccgagccga acctggcgca
     1381 cgacagctcg acgaacacgc tcatccggcg gtatcgctca cagaaaaaat agccagcgtt
     1441 tcctttgctc gtcggcttgc ccgccattgg catgccgcag aaactcctgc acgctcggcc
     1501 gccgcagaag attcagctcc atcgcgccaa ggatgagatt gatcggatcg caggcgacaa
     1561 agcagatcga ctgcagcgtg gcgacatggc cgagcccctg cgtatcgcgg gccgctccgc
     1621 gggccaccgc catcacgccc acgagcagcg cgaaatacat cgtcggaaac aggaacggcc
     1681 cccacaggaa cagcagcgag atcggaatcg tcaaagccgc acagcccccc aggaacatca
     1741 gcccccagca gagcttcagt tcggtgggtg ggcactcgcc gagcgcagaa gtcaatatcg
     1801 agattcgata tcgccgattc cgcgtcgaaa cgatccccca aaccgccgcc cagcccgcaa
     1861 agccggcagt gaacaggagg atctgcagcc aacgccagtc ggttgtgagt ctgaagcgca
     1921 cggctccatc gggaaggctg cgaattgtga tgcctccgcg ctccagcgaa gccatggcgc
     1981 gcccttccgg agcaagcgcg agcctcgcac tcgcgaacgg caccgagtct tccggaagga
     2041 actgatacgg agtgtacccg tcgaaatcgg ttcgagacac gaactcgccg ttcgatgcat
     2101 cgtagatttc gagcgcatcg aatcccacag cggcaatctg tgaaccctca ttgacaaagg
     2161 ccacggaatg gacaggcgac acgcctgtgg aaatcgtgcg gaattggccc gggttgagca
     2221 gatcaaagac agtaacggtt ttccacgggc cgctcactgc ccaatccgcc gtcacaagtt
     2281 tacccgcctc gaccgcgtgc gacgttacat tggtggtatg cgtgattgtc tgcaatcgac
     2341 ctcgcgctcc caagtccaga acgtgcaaac cgccatctcg gtctgagaac gcggcgcaac
     2401 caccatcagg tgagaccaat agctgcacaa
//
LOCUS       DASUGB010000517         8363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1587527, whole genome shotgun sequence.
ACCESSION   DASUGB010000517 DASUGB010000000
VERSION     DASUGB010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587527"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFV87_09905"
     CDS             complement(<1..938)
                     /locus_tag="VFV87_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104114.1"
                     /translation="MQRPFYAFGALLLALTLTMLAINAGGRAAVVRQQLPQERPGSAT
                     SDLPQREVRKPRFCSLVVSLPADEDEILADAIALINESPCYATGCGLDRATGEVYPRY
                     SDGECDWQCPGALAWPKPAARPMQPVRLVQAVRDPTTLRDETSRDCLSHYDPAYDGAV
                     YSDENPEADVAGARSQRLPAGVGLDFRSHYDTAYDEIVYSEAERPRAQHGVEALLLPA
                     DRRLGNARTLLIGLANQLMWKLEKAAGLGEWIGPAQAGGKRIDWNDYSRLLDESLGSF
                     PLDSHAAESTTAVRSSGWLGDFTATSLHGASDLLESV"
     gene            1408..1887
                     /locus_tag="VFV87_09910"
     CDS             1408..1887
                     /locus_tag="VFV87_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104115.1"
                     /translation="MLGIQARGFALHKATTEPKKLIVVVGTWIWMVPLLIAGLFFFFL
                     GAGALFYGVRDGRAFESLFGLPCGLLGVLFVGIAVIILYRTTTGYVKHRSGGRHGELE
                     SMPDGEEDAGDVETCLACGKELAPEATRCPACGWTFGAGTEGDSCSQRQSNAAPSQQ"
     gene            2225..5794
                     /locus_tag="VFV87_09915"
     CDS             2225..5794
                     /locus_tag="VFV87_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX5104116.1"
                     /translation="MAIRVALHHQTEYRYDRPVTIEPHVVRLRPAPHCRTPIRAYSLK
                     VEPGEHFLNWQQDPYSNYLARLVFPKPARSLLFTVDLVAEMISINPFDFFIEAYAEKY
                     PFQYDGILARELIPYLETQPPGPELASLIRETKQSGGQVIDYLVSLNQKLNKLIGYVI
                     RLEPGIQSCEETLTKRTGSCRDSAWLLVQLLRHQGLAARFVSGYLIQLTADVKSLDGP
                     SGPESDFTDLHAWAEVYLPGAGWIGLDPTSGLLAGEGHIPLACAADPITAAPITGSYS
                     WSKSTDNDYCREEFRHSMKVTRIHEDPRVTRPYTERQWRQIDDLGREIDEMLRARDVR
                     LTMGGEPTFVSIDDRDGEEWNTAALGPHKRERAGVLLRRLRDRFAPQGLLHFGQGKWY
                     PGESLPRWALGCYWRLDGQPMWQDPALVAEDGKNYDASPQSARWFVTTLARKLGVDPS
                     LAIPGHEDAWYYLWRERRLPTNVDPLQSKLDDAEERARLTKIFNQGLDKIVGFALPVQ
                     RVHTPEGSYWVSGKWFLRREHMFLIPGDSPMGYRLPLDSLPWVAPGDREPVIEHDPFE
                     HLDPLPPHSSYLASGGRQPAESAGAALAPRFQSRESLAPSADFSGGLRPPLADWRPPL
                     AERAPIGHSQPGVVRTALCIEPRGGTLFVFMPPIGCLEDYLDLVAAIEATAAELALPV
                     QLEGYSPPSDHRLQHLKITPDPGVIEVNIHPSHDWEQLRENTHIIYEEAHHSRLSTEK
                     FMLDGRHTGTGGGNHVVLGGPSPGESPLLRRPDLLRSLVGYWHNHPSLSYLFSGMFVG
                     PTSQAPRVDEARNDNLYELEIAFRQIPERCAAGDSRPWIVDRVFRHLLVDLTGNTHRA
                     EFCIDKLYSPDSLDGRRGLLEFRAFEMPPHWQMSLVQQVLLRALVARFWDQPYQAPLV
                     RWGTELHDRFMLPHFVLQDFQDVLFELRQAGLPLQEAWFAPHFEFRFPQHGHFTQRGV
                     HVELRQAVEPWHVLGEEATGNGTARFVDSSLERLQIKVSGLTDARHIVTCNGRRVPLH
                     PTGVNGEFVAGVRYRAWQPPSCLHPTIPVHAPLVIDLLDSWMGRSLGGCTYHVAHPGG
                     RAHDTFPVNANEAEGRRVARFFSFGHSPGPIAIPPEVRHAENPLTLDLRQASAETEFP
                     LRAVRSVDSAPTAAADSNGSHHRGNGASKLYSPQNVEREHMFS"
     gene            complement(5842..6609)
                     /locus_tag="VFV87_09920"
     CDS             complement(5842..6609)
                     /locus_tag="VFV87_09920"
                     /inference="COORDINATES: protein motif:HMM:NF037407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104117.1"
                     /translation="MADRRTRRVTRCAGGVCQGERVLARHCLNSARPRAAAKRETMKE
                     EQLLLQIAQQYRQDGYTVWLRPAGNERPPFLANFDVDMIAARGNENVVVEVREAGTNG
                     EASARLGYLASIVNAEPNWRFDVVVPGEDASRMGEERNATEIRALMAEAQQLVGLGML
                     EPALLVAWAALEAAMREAAKREGIPLEERNPQFVLTTIFAEGLVSREDYEIVRQTMRT
                     RNGVAHGLKERQLSSDIPSALIQIAERLLNSQPAASA"
     gene            7161..7655
                     /gene="fae"
                     /locus_tag="VFV87_09925"
     CDS             7161..7655
                     /gene="fae"
                     /locus_tag="VFV87_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEX5104118.1"
                     /translation="MSMFIGEGLFGEGNEIAHIDLLIGDKDGPVGHAFANALARQSEG
                     HTNLLAVITPNLAVKPATVMITKVTIKGMKQAVQMFGPAQAAVAKAVADQVAAGVIPK
                     NKAEDLVIVCGVFIHPAAADDKKIYQYNYEATKLAISCAMTGKPTADEMIAGKEQVHP
                     FRGF"
     gene            7709..>8363
                     /locus_tag="VFV87_09930"
     CDS             7709..>8363
                     /locus_tag="VFV87_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEX5104119.1"
                     /translation="MKPKILIQFDPDPHASVFDAVVAADSGVDQLLQYAAVQPEQVQG
                     LVHGAMFTRGPQDLHNTAIFIGGSSVAAGEELLKQTTQCFFGPMRVSVLLDANGANTT
                     ASAAVLAAAKHVPLVGAVATVLAGTGPVGQRAARLLARQGAEVRLASRSLEKAAAACE
                     SIAARVPGAKLKPVKTTSAGETAAALDGVQIAIAAGAAGIELIPADVRRNCESLKVAI
                     "
BASE COUNT         1461 a         2787 c         2581 g         1534 t
ORIGIN      
        1 accgactcca gcagatcgct cgctccgtgg agagacgttg ctgtgaaatc gcccaaccag
       61 ccgctagatc gaacagccgt ggtcgattcc gccgcatggg aatcgagcgg aaaactgccc
      121 agcgactcat cgagcaaccg cgaataatcg ttccaatcga ttcgcttacc gccggcttgt
      181 gcgggcccga tccactcacc caatccagcg gctttttcca gcttccacat caattgattc
      241 gccaggccga tcaacagcgt ccgagcgttg cccaggcgtc gatccgcagg cagcaggagc
      301 gcctcaactc cgtgttgcgc cctgggccgc tcggcctcgc tataaacgat ttcatcgtaa
      361 gccgtgtcgt agtgagatcg aaaatccagg ccaacaccgg ccggaagccg ttgagaccga
      421 gctcctgcca catccgcttc aggattctca tcggaataca cagccccgtc ataggccggg
      481 tcgtaatgcg aaaggcagtc gcggctcgtt tcgtcgcgta gcgtggtggg gtcccggacc
      541 gcctgcacca gtcgcacagg ctgcatcggc ctggccgccg gcttcggcca ggcaagcgcc
      601 cccgggcatt gccagtcgca ttcgccgtcg ctgtagcgcg gatacacttc gcccgtcgcg
      661 cgatccaacc cgcacccggt ggcatagcag gggctctcgt tgatcagagc gatcgcgtcc
      721 gcgagaatct cgtcctcgtc cgccggcaga ctgacgacga ggctgcagaa ccgcggcttc
      781 cggacctccc tctgaggcaa gtcgctcgtc gctgagccag gacgctcctg cggcaattgc
      841 tgacgaacga ctgccgcacg gccacccgcg ttaatcgcca gcatcgtcag cgtcagggcc
      901 aggagcagcg ctccgaaagc gtagaatggc cgttgcacgt tgggctcctc caaggtgtca
      961 ctccgcggcc tgctgtgagc cgaattcatc ccgcaccttc ccgctcgccg gtgcacagtc
     1021 tcgtacagtg gctatcggag tctgccgctt ggggtcatta ggcaatcggc agcgggcggg
     1081 caacgcgtag cgagcgcgct gccgtttgct ccggacagcc gcaaacgact gcccttcacg
     1141 atcgtgcggg ttgctccggc tgagcaaacc caagccggtc gagctcaagt aactccagaa
     1201 ggcctcgtcg agcgctggtc gtgtcgtctt ctcggcccga caaccgctat attgattggc
     1261 cggcggccat gcagaactcc catggatgac tcgtcaaatc cagcgcctct cctgatctgc
     1321 acgtcgtgcg gtgtccacca atccgcggca gcacatcttt gcgcccattg cggagcgccg
     1381 ctgacgccct tcgcacatag cgatcctgtg ctcggaattc aagcgcgagg gtttgcgctg
     1441 cacaaagcga ccacagagcc caagaagctg atcgtcgtag tcggaacctg gatctggatg
     1501 gttcccctgc tcattgctgg actgtttttc ttcttcctgg gtgcaggagc cctcttctac
     1561 ggcgtacgag acggacgagc tttcgagtcg ctgtttggac ttccctgtgg attgctcggc
     1621 gttctgtttg ttggtatcgc cgtgatcatt ctctatcgaa cgacgaccgg ctacgtgaaa
     1681 catcgatcag gcggcagaca tggggagctt gaatccatgc cggacggcga ggaagatgcc
     1741 ggcgatgtgg aaacatgcct ggcttgtgga aaggagctcg cgccggaagc cactcgttgt
     1801 ccggcgtgcg ggtggacatt tggagctggt acagagggcg attcgtgcag ccaacggcaa
     1861 tcgaacgctg caccttcgca gcaataggac ttgctgggct tacgggcgaa ctttgatcag
     1921 acgttgggca gcacacgttt aatttcgttg cccaggctgg cgtgggtgct cagccacaaa
     1981 atcttccgtt tcaagaactt gcgtgccggg ggcggcgacc gatcagcaac ggaacgacaa
     2041 ttgcttgttg cgcgccggat tggtgccacc tgtgccgcca ctaacgggcg tatgagcggc
     2101 tgagctgccc aattacggct ggtggtccga tgggctttga caccttatcg tcgctggccg
     2161 ataaccggta aatcttgcct cgtccgttat cctccgacgc ggcacacgga gtgtgccaac
     2221 aactatggcc atccgcgttg ccctgcatca ccagacggaa tatcgctacg accggcccgt
     2281 gacgatcgag ccgcatgtcg tccgcctgcg gccggccccg cactgccgca cgccgatccg
     2341 ggcctattcg ctgaaagtcg agccgggcga gcactttctc aactggcagc aggacccgta
     2401 cagcaactat ctcgcccggc tggtgttccc caagccggcc cggtcgctgc tgttcaccgt
     2461 cgatctggtg gccgagatga tctcgatcaa cccgttcgac ttcttcatcg aggcgtacgc
     2521 ggagaagtat ccgttccaat atgacgggat cctggcccgc gagctgattc cctacctcga
     2581 aacccagccg ccagggccgg aacttgcctc gctgatccgc gagacgaagc agagcggcgg
     2641 gcaggtcatc gactacctcg tcagcctcaa tcaaaagctc aacaagctga tcggctacgt
     2701 catccgcctg gagccgggca ttcagtcgtg cgaggagacg ctgaccaagc gcaccggctc
     2761 gtgccgcgac agcgcgtggc tgctcgtgca gctattgcga catcagggcc tggcggcgcg
     2821 gttcgtctcg ggctatctga tccaactcac ggccgatgtg aagtcgctcg acggaccgag
     2881 cggcccggag agcgacttca ccgacctgca cgcctgggcc gaggtctatc ttcccggcgc
     2941 gggctggatc ggtctcgatc cgacgagcgg cctgctcgcg ggcgaagggc acattccgct
     3001 cgcctgcgcc gccgatccga tcaccgccgc gccgatcacc ggcagctact cgtggtccaa
     3061 gagcactgac aacgattatt gccgcgagga gttccgccac agcatgaagg tgacgcggat
     3121 tcacgaggac ccgcgcgtca ccaggcccta taccgagcgg caatggcggc agatcgacga
     3181 cctggggcgc gagatcgacg aaatgctgcg cgcccgggac gtccgcctga cgatgggggg
     3241 cgagccgacg ttcgtcagca tcgacgaccg cgacggcgag gagtggaaca ccgcagccct
     3301 cgggccccac aagcgcgagc gggcgggcgt cctcttgcgg cgattgcgcg accgcttcgc
     3361 gccgcagggc ctgttgcact tcggccaggg gaagtggtat ccgggcgaat cgctgccgcg
     3421 gtgggcgctc ggctgctact ggcggctcga tggccagccg atgtggcagg atcccgcgct
     3481 ggtggccgag gacggcaaaa actacgacgc cagcccgcag tcggcccgct ggtttgtgac
     3541 cacgctggcc cgcaagctgg gcgtcgatcc gagcttggcg attcccggcc atgaggacgc
     3601 ctggtactac ctgtggcgcg agcgacggct gccgaccaac gtcgatccgc tgcagagcaa
     3661 gctcgacgac gcggaagagc gggcccggct gactaaaatc ttcaatcagg ggctcgacaa
     3721 gatcgtgggc tttgccctcc cggtccagcg cgtgcatacg ccggaaggct cgtactgggt
     3781 cagcggcaag tggttcctcc gccgcgagca catgttcctg atcccgggcg actcgccgat
     3841 gggctaccgc ctgccgctcg atagcttgcc ctgggtcgcg cccggcgacc gcgagccggt
     3901 gatcgagcac gaccccttcg agcatctcga cccactgccg ccgcactcca gttatctggc
     3961 gagcggcggg cgtcagcccg cagagagcgc aggcgcggca ctcgccccgc gttttcaatc
     4021 tcgcgaatcg ctggcaccca gcgcggactt ctcagggggc ttacgccccc cgctcgccga
     4081 ttggcgcccc ccgctcgccg aacgcgcgcc gatcggccat tcccagcccg gcgtcgtccg
     4141 cacggccctc tgtatcgaac cccgcggcgg aacgctgttc gtcttcatgc cccccatcgg
     4201 ctgcctggag gactatctcg acctggtcgc ggccatcgaa gccaccgcgg cggagctcgc
     4261 gctcccggtg cagctcgaag gctactcccc gcccagcgac caccggctgc agcacctgaa
     4321 gatcaccccc gaccccggcg tcatcgaggt caacattcac ccgtcgcacg actgggagca
     4381 gcttcgggag aacacgcaca tcatctacga ggaggcccac cactcgcggc tctcgacgga
     4441 aaaattcatg ctcgacggcc ggcatacggg caccggcggc ggcaatcacg tcgtcctcgg
     4501 cgggccgagc ccgggcgaaa gcccgcttct gcggcggccg gacctcttgc ggagcctcgt
     4561 cggctattgg cataaccatc cgtcgctgtc gtacctgttc agcggcatgt tcgtcgggcc
     4621 gacaagccag gcgccgcgcg tcgatgaggc ccgcaacgac aacctgtacg agttggaaat
     4681 cgccttccgg cagattcccg agcgatgcgc cgccggcgat tcgcggccgt ggatcgtcga
     4741 tcgcgtcttc cgccatttgc tggtcgatct gaccggcaac acgcaccggg ccgaattctg
     4801 catcgacaag ctctactcgc ccgactcgct cgatggccgc cgcggccttc tggaattccg
     4861 cgcgttcgaa atgccgccgc actggcagat gagccttgtg cagcaggtcc tgttgcgcgc
     4921 cctggtcgcc cgcttctggg accagccgta ccaggcgccg ctcgtccgct ggggaacgga
     4981 attgcacgac cggttcatgc tgccgcactt cgtgctgcag gattttcagg atgtgctgtt
     5041 cgagctgcgg caggcgggcc tgccgctgca ggaagcctgg tttgcgccgc acttcgagtt
     5101 tcgcttcccg cagcatgggc atttcacaca acgcggcgtc cacgtcgaat tgcggcaggc
     5161 ggttgagccg tggcacgtgc tgggcgagga agcgactgga aacggcactg cgcggttcgt
     5221 cgattcttcg cttgagcggc tccagattaa ggtcagcggc ctgaccgacg cgcggcacat
     5281 cgtgacctgc aatggccgcc gcgtgccgct gcatccgacg ggtgtgaatg gcgagttcgt
     5341 cgccggcgtg cgctatcgcg cctggcagcc gccgtcgtgc ctgcacccga cgattccggt
     5401 ccacgcgccg ctggtgatcg atcttttgga ctcctggatg ggccgttcgc tcggcggctg
     5461 tacataccac gtcgcccatc ccggcggccg ggcgcacgac acgtttcccg tcaacgccaa
     5521 cgaagccgag gggcgccgcg tggcccggtt cttctccttc ggccactcgc ccgggccgat
     5581 cgcgattccg ccggaagtcc gccacgccga gaacccgctg accctcgacc tccgccaggc
     5641 ctcggcagag acggaattcc cgctccgcgc cgtcaggtca gtggacagcg cacctacagc
     5701 cgctgcggat tccaacggct cgcaccatcg cggcaacggt gcctcgaagc tctactcgcc
     5761 gcagaacgtc gagcgcgagc atatgttttc gtagccgacc gccgaccacc ggctcgtggt
     5821 ccagcgccat tgcaacctac gttatgcgct cgctgccggt tgtgagttga ggagccgttc
     5881 agcgatttgg attagcgcgg agggaatgtc gctgctcagt tgccgctctt taagtccgtg
     5941 tgcgacgcca tttcgagtgc gcatcgtttg tcggacgatc tcgtagtctt cgcgcgacac
     6001 cagaccctct gcgaatatcg ttgtcagcac gaactgaggg ttccgttctt cgagcggaat
     6061 cccttcgcgc ttggcggcct cgcgcattgc ggcttcgagg gcagcccaag cgacaagcag
     6121 cgccggctca agcattccca atccaacgag ctgctgggct tcagccatca gcgcccgaat
     6181 ctccgtcgcg tttcgctctt ctcccatgcg gctggcgtct tccccaggaa cgacaacgtc
     6241 gaaccgccag ttaggctcgg cgtttacaat gcttgcgaga taccccagac gagcgcttgc
     6301 ctcaccgttc gtcccggcct cgcggacctc cacgacgacg ttctcattgc cccgagcggc
     6361 gatcatatcc acgtcgaaat tggctagaaa cggcggcctt tcgttgccag cggggcggag
     6421 ccataccgtg tagccatcct ggcgatactg ttgcgcaatc tgaagaagca gttgttcttc
     6481 cttcatcgtt tccctcttag cggctgcacg tggtcgggcg ctgttgagac agtgacgtgc
     6541 aagaactctt tccccctggc aaacgcctcc agcacaacgg gtgacgcgcc gcgtgcgacg
     6601 atcggccaat caatgccggt tttgcgcacc acgacgtcag tgacttgccg gtcatcctca
     6661 tacaccaatt gattcatggc atcctggatc ggttttacga gattgtcgtt atccattcgg
     6721 attgcggtac cctcatggta gtaggcgacc gtaacctgaa gtcgctcctt cgtcggtttc
     6781 cgattccccc acgcggccgc cgccgcatct cgcacggcca acttccacgc cgtcaatttg
     6841 gaccggttcc tggattggtg ggaaacaggc ggtcccccaa tggtgaattc caggaatctc
     6901 atctgccgag tctctcggaa ttcgcgatcg cgcgtttcgc actagcttca attatcacac
     6961 gttggcgcca gatcgcaaac cagaagacgg aaaggtggta ccgctgggtg gggccgccgg
     7021 gtagttggca tcctccgcgc cgtttgctac cttgcttccc aacatcaagc ggcacaaggg
     7081 cttgctggcg gagggggttc tccgccgcgg ctgacgctgc gggcgctgga acaccaatca
     7141 ctcaaacgcg gagaacgact atgtccatgt tcatcggcga aggccttttt ggcgaaggca
     7201 atgaaatcgc tcacatcgac ctgctcattg gcgacaagga cggcccggtc ggccatgcgt
     7261 tcgccaacgc cttggcccgc cagagtgaag ggcacacgaa tctactcgcc gttatcacgc
     7321 ccaatctcgc cgtcaagccg gcgaccgtga tgatcaccaa agtcacgatc aagggaatga
     7381 agcaagccgt gcagatgttc ggcccggccc aggccgcggt cgcgaaggcg gtcgccgatc
     7441 aggtcgcggc gggcgtgatt cccaagaaca aggccgagga cctcgtgatc gtctgtggcg
     7501 tcttcatcca tcccgccgca gccgacgaca agaagatcta ccagtacaac tacgaggcga
     7561 cgaagctcgc catctcctgt gccatgacgg gcaagcccac ggctgacgag atgatcgccg
     7621 gcaaggaaca ggtccacccg ttccgcgggt tctaggcttc tgccgaagtt tgcagtcgta
     7681 gccgcactcg ccagagtgcg ggcgcgcgat gaagcccaag atcctgatcc agttcgatcc
     7741 cgatccgcac gccagcgtct tcgacgcggt ggtcgcggcg gacagcggtg tggatcagct
     7801 gctgcaatac gcggccgtcc agccagagca ggtgcagggg ctggttcacg gggcgatgtt
     7861 cacgcgcggc ccgcaggact tgcacaatac ggccatcttc atcggcggct ccagcgtggc
     7921 tgccggcgag gagttgctga agcagacgac gcagtgcttc ttcgggccga tgcgcgtctc
     7981 ggtgctgctc gacgccaacg gggccaacac gacggcttcg gcggctgtgc tggcggcggc
     8041 gaagcacgtg ccgctggtcg gtgcggtggc cacggtgctg gccgggacgg ggccggtcgg
     8101 tcagcgtgcg gcccggctgc tggcgcggca aggagcggag gtgcggctgg cttcccggtc
     8161 gctcgagaaa gccgcagcag cctgcgaatc gatcgccgct cgcgtccccg gcgcgaagct
     8221 gaagcccgtg aagaccacct ccgctggcga aaccgcggcg gcccttgatg gggtgcaaat
     8281 cgcgatcgcc gccggcgccg caggcatcga gctcatcccg gctgacgtgc gccgcaactg
     8341 cgaatcgctg aaagtcgcca tcg
//
LOCUS       DASUGB010000518         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1307954, whole genome shotgun sequence.
ACCESSION   DASUGB010000518 DASUGB010000000
VERSION     DASUGB010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307954"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFV87_09935"
     CDS             complement(<1..684)
                     /locus_tag="VFV87_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5104120.1"
                     /translation="MESFDAIIVGGGPAGSSCAWKLVRGGLIVLVLDKKAFPRVKPCA
                     GWITPQVVESLQLDEHDYRAGRVWQPITGFRCGVIGGRDVEVRYERPISFGIRRYEFD
                     DYLLRRSGAALSLAESVEAIERDGETWVINGRCKAPLLIGAGGHFCPVARRLGARPEA
                     AAVVYAQEAEYQADPEASGSVSAQMPALYFCPDLLGYGWCFRKADYLNVGLGRLDAKG
                     LSSHVAAFCD"
     gene            829..1575
                     /locus_tag="VFV87_09940"
     CDS             829..1575
                     /locus_tag="VFV87_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104121.1"
                     /translation="MVLQTTPDLSAIYVPDNHALVRVSGVHKYFRRGSERIDVLQGLD
                     LNVAEGEFFGLMGPSGSGKTTLLNLIAGLDRPSEGQVWVGDHCISEMSESQLARWRTR
                     HVGFVFQFYHLLPVLSAYENVELPLLLLPLSGSERRQQVEIAMELVGLKDRMRHRPGQ
                     LSGGQQQRVGIARAIVTDPELIVADEPTGDLDAKSAEEILDLLEELRAGLEKTIILVT
                     HDPRAARRTDRILRLEKGVLVDGLSAVTEN"
     gene            1747..2994
                     /locus_tag="VFV87_09945"
     CDS             1747..2994
                     /locus_tag="VFV87_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104122.1"
                     /translation="MKFLFLILKNVRRNLLRSSLTALGTMALVLVVTLVWSLLAFLDQ
                     ITSEKKKNLKAIVSERWQFPSQMPFSYADTLAEGAAREPGDVQPLDSMTWQFYGGVLD
                     KENITRNTRMFAFAMEPRKLLTMMDELDSLPADQKRDLEATVQKMEANRQAIAMGKDW
                     MRILNKRVGDRLTLYGLNYRGIDLEFEIVGVFPPGRYDKSIVMNRDYLNGEVDKWPRE
                     HNGQPHPMADRRLGLVWLRVPDMATYSQVAEQIENSPYYSSPTLKVETASSGIASFLD
                     AYRDLIWGMRWLLTPACLVSLSLVIANAISISVRERRKELAVMKVLGFRPMQILVLVL
                     GESLLLGAGAGFVAAWLTWYGVNYIVGGMPFPIAFFPRFYIAGGALWWGPAIGGLAAL
                     AGSAGPAWSACTVKVTDVFAKVA"
BASE COUNT          624 a         1094 c          998 g          593 t
ORIGIN      
        1 atcacaaaac gccgccacgt gcgacgacag ccctttcgca tctaaccgcc ccagtcccac
       61 gttcaagtag tccgctttgc gaaagcacca gccgtaccca agcaaatccg ggcaaaaata
      121 gagcgccggc atctgggccg agacgctgcc gctggcctct ggatcggcct gatactcggc
      181 ttcctgggca tagacgaccg ccgccgcttc cggccgcgcc cccagccgcc gcgcaaccgg
      241 gcagaaatgc ccgccggcgc caatcaagag cggcgccttg cagcggccgt tgatcaccca
      301 cgtttcgccg tcgcgctcga tcgcctccac cgattccgcc agcgacaggg ccgcgcccga
      361 ccgccgcaaa agatagtcgt caaactcgta ccgccgaatc ccaaagctga tcggccgctc
      421 gtaccgcacc tccacatccc gaccgccaat cacgccgcaa cgaaaaccgg tgatcggctg
      481 ccagacgcgg ccggcacggt agtcgtgctc gtccagttgc agcgactcca caacctgcgg
      541 cgtgatccac cccgcacagg gcttcacgcg gggaaacgcc ttcttgtcga gtaccaggac
      601 gatcagcccg ccgcggacca gcttccacgc gcacgacgac cccgcaggtc ccccgccgac
      661 gataatggca tcgaacgatt ccacgattct cgcctattgg tagccgcgtt cgccagaacg
      721 cgggtgattc gcacaccggc ccgcactctg gcgagtgcga ctacctccat cggcattcta
      781 gtcggcaatg gacggacgtt cttgtctgct gcggaaccgc gtgcaaaaat ggtccttcaa
      841 acgacccccg acttatcggc gatctacgtg ccagacaacc acgccctcgt tcgcgtctcc
      901 ggcgttcaca agtatttccg ccgcggcagc gagcggatcg acgtcttgca ggggctcgac
      961 ctgaacgtcg cggaaggcga attcttcggc ctgatgggcc ccagcggctc ggggaagacg
     1021 acactgctga atctgatcgc cggcctcgac cgccccagcg aaggccaggt ctgggtcggc
     1081 gaccactgca tctccgaaat gtccgaaagc cagctcgccc gctggcgcac gcggcacgtc
     1141 ggcttcgtgt ttcagttcta tcacctcctg ccggtcctct cggcttatga aaacgtggag
     1201 ctcccgctgc tcttgttgcc cctttcgggg agcgagcggc ggcagcaggt cgaaattgcg
     1261 atggagctgg tcggcctgaa ggatcgcatg cggcaccgcc ccgggcagct ttccggcggc
     1321 cagcagcagc gggtcggcat cgcccgggcg atcgtcaccg atccggaact gatcgtcgct
     1381 gacgagccga cgggcgacct cgacgcgaaa tcagccgaag agatcctcga cctgctcgaa
     1441 gagctgcgcg ccggcctgga aaaaacgatc atcctcgtca cgcacgatcc ccgcgccgcc
     1501 cgccgcaccg accgcatcct gcgactggag aagggcgtgc tggtcgatgg gctgtcggcg
     1561 gtgacggaga attgacctgg cggctagaag ccgccgctac gactgacgct cggtcccacg
     1621 ctccgcgtgg gaccgcaagc cctgacgctc cgcgtcctgg aagcgacgca gagcgtcgaa
     1681 tgcgtgcgtt cccacgcaga gcgtgggaac gagaccgccc gaatgagact atccgttgag
     1741 acgcccatga aattcctctt cctcatcctg aaaaacgtcc ggcgaaacct gctccgctcg
     1801 tcgctgacgg ctctgggcac gatggcccta gtgctggtgg tgacgctcgt ctggtcgctc
     1861 ctggcgtttc tcgaccagat caccagcgag aaaaagaaga acctcaaggc gatcgtcagc
     1921 gagcggtggc aatttcccag ccagatgcct ttcagctatg ccgacacgct ggccgaaggc
     1981 gccgcccgcg agccgggcga cgtgcagccg ctcgattcga tgacctggca gttttatggg
     2041 ggcgtgctcg acaaggagaa tatcacccgc aatacgcgga tgttcgcctt cgccatggag
     2101 ccgcgcaagc ttttgacaat gatggacgag ctcgactcgc tgccagccga ccagaagcgc
     2161 gacctggagg ccaccgtcca gaagatggag gccaaccgcc aggccatcgc gatgggcaag
     2221 gattggatgc gaatcctcaa caagcgggtc ggcgatcggc tgacgctcta tggactcaac
     2281 tatcgcggca tcgatctgga atttgaaatc gtcggcgttt ttcctcccgg ccgctatgac
     2341 aaatcgatcg tcatgaatcg cgactatctc aacggtgaag tcgataagtg gccccgcgag
     2401 cataacggcc agcctcaccc gatggcggac aggcggctgg ggctcgtctg gctgcgggtg
     2461 ccggacatgg cgacttattc gcaagtggcc gaacagattg agaactcgcc gtactattcg
     2521 agcccgacgc tcaaggtgga gacggcatcg tcggggatcg cgtcgtttct cgatgcctat
     2581 cgcgacttga tttgggggat gcggtggctg ctcacgccgg cctgcctggt gtcgctctcg
     2641 ctggtcatcg ccaacgcgat cagcatcagc gtccgcgagc gccgcaagga gttggcggtg
     2701 atgaaggtac tgggcttccg gcccatgcag attttggtgc tggtgctggg cgaatcgctg
     2761 ctgctcggcg cgggtgcggg atttgtggcc gcctggctga cgtggtacgg cgtgaactac
     2821 atcgtcggcg ggatgccgtt tccgatcgcg ttcttcccgc ggttttatat cgcgggcggc
     2881 gcgctctggt ggggacccgc catcggcggc ctcgccgccc tcgccggcag cgccggcccc
     2941 gcctggagcg cctgcaccgt gaaagtgacc gacgttttcg cgaaggtagc ttaaacgaac
     3001 tccccgtcgg gcttgccccg gcggagaaat gactgacggc tacggccgaa ttacgacgaa
     3061 caatccaatt caatccccgc cgggcttgcc ccggcggaga aatgactgac cgctacggcg
     3121 atgcccgacc cgatttacac tagcgagaac tgccacccgg cctaccaact tagaggttct
     3181 aacacaacct ggaattgaca ttattcgcgt ggtcagattt tggtattctt cgcggtggga
     3241 ttagcttcgt ggtctctgtc atggaggaca ctgcgatgtc gaaaccactt cttcccgatg
     3301 agctttggg
//
LOCUS       DASUGB010000519        11061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1307956, whole genome shotgun sequence.
ACCESSION   DASUGB010000519 DASUGB010000000
VERSION     DASUGB010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307956"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFV87_09950"
     CDS             <1..773
                     /locus_tag="VFV87_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX5104123.1"
                     /translation="TEVSLDQLEGTVLDVGPESVQFEYDGEKIDVPRGKLEAVVYYHP
                     ARREFSAPACKLVDVGGSAWSLKSLELAEGQVRGSTVGGVAIDFPLSAVAKIDYSVGN
                     VFFLAELEPDTGAGAPAVSLQPASMAFKFGRIFGVRAGPPLGASRFQIGGTAYDNGLS
                     LHSPASIVYRVPEGARWLRAEAGVDDSVVAPGEFDLVIQGDGKELLRHSFGQSEDRKP
                     LPINLDIAGIRRITISLDPADGQDIGDQLNLCKARMTK"
     gene            770..2596
                     /locus_tag="VFV87_09955"
     CDS             770..2596
                     /locus_tag="VFV87_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5104124.1"
                     /translation="MRLLIQSTLAFALLGLPLTIRAEVPQPVLEAEQARIASIEKAVK
                     TAVAVFAKGGNGGGSGVVISPDGYALTNFHVVQPAGDHMKCAMADGELYDAVIVGIDP
                     TGDVAVIKLLGRDDFPYSIMADSNEVQVGDWCFAVGNPFLLATDFQPTVTYGIVSGTH
                     RYQPPAGTLLEYTDCLQTDASINPGNSGGPLFNANGDLVGINGRGSFEKRGRVNVGVG
                     YAISINQIKNFLGCLKSGRIVDHATLGATVAMSDDGTVRVSNILESSDAYRRGLRYDD
                     EIVSFGGRPIDTQNAFKNVLGIYPKGWRVPLVYRRGGERQEILVRLTGVHAEEELTEL
                     LQRRPEQRPMPQPRPGGRPMPMPQPGDDPEKPGEGPKPAEPPKPGEPKPLPKLPIELG
                     KKEPPIPPEIQKLIVARPGYANYYFNEQNRDRVWSAFSKTSGDFSAAAGEWKLTGELE
                     GGGKVEVTLGDETSTGNFPQGAVKLDGGQDLDQQLGPAGSGGLVAALHLWRTLLVTGP
                     QKFGLVYYYGTAPYPGIESQADVLVATRNVAEVNLVFDPKSGQLAILEMVADPDADPC
                     EIRFGDYRDVGGRQVPHRLEVRHGDAAFGTILLSQIEVGAKP"
     gene            2593..3621
                     /locus_tag="VFV87_09960"
     CDS             2593..3621
                     /locus_tag="VFV87_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5104125.1"
                     /translation="MTRRTRFCSMILALAAIGLLGLPYAAWAQTSFSETIDQTQPKIV
                     KIFGAGGLRGLEPYQSGFLISADGHILTVWSYVLDSDTVTVYLNDGRKLEAEVVGMDP
                     RTEIAVLKIDAQNLPHFNLDEAVALNAGARVLAFSNLYGVALGNEPASVLHGIVSAKT
                     DLAARRGAFETNYRGQAYVLDAMTNNPGAAGGALTDRRGRLAGLLGKELRSSLSNIWL
                     NYAIPISELATTADEIIAGKFRPAPRDEGEKKPKEPHTLALLGINLVTDFLTKTPPFI
                     ESVKPGSPAAKAGLRTDDLILFVNSRVVQSCKLLVDELSYIDRLDPVRLTVQRGQDLL
                     EVELQPVP"
     gene            3666..7352
                     /locus_tag="VFV87_09965"
     CDS             3666..7352
                     /locus_tag="VFV87_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5104126.1"
                     /translation="MPHRWSAPRTIALILIALACVAAAIYFASRFGGGPSADEVRQLV
                     EEKNVGLGYLENQTLDDAVSSFEKIRAKLPADPLSWRNLAIARVLALGDDGADATLEK
                     LAAATSALKEVSRLEGASAPYHWLSLQTALAAEDFAAAQTHLNAIQAEESGDAAAWFA
                     KFSALERQTGEKLDPPALQALEKACQLRPENAWLRVEWLRAVGTALLQLPEDAGRADA
                     AAGAWGDLAAQLDQGQRAIEPFAPLIQTHTKVDALALLSEAKQAVADRQWREAGQRML
                     TLANVLVPHAALDRMQVRRHPLEFVATEFQPQIMRGMPPTEEEKPIAVSFRPGGEGSE
                     SLSAATKGAVDFALADFDLDGRLDVVALLPDKIVVLSRGIEGQDWQTIASAEVSGAEQ
                     LLVQDLDSDFDETVLAVHGGGHVEGQKEKDKPAGAPCPTADVDIVAYGQSGVMLLENR
                     FDPATRSRTLVAVPGEKLPAALMDVATLAAADLEGDGDLDLVLAAGDTLHRWMNGGEW
                     TFLAAASDERPGVTQILPLDFDRDIDIDLLIASPNEAGWLENVRHGGFRWRPFARDFP
                     DLATATALESVDFDGTASWDLVAATDQGLRLVTTRTPQPGDIRLKESLSIASAKPDRL
                     AAFDYDNDGHDDLLSWTKEQTTVARCEPASGAAPRTWNVETPAYRSAGPAKSAAQGDL
                     DNDGDLDIAVLDDRGVSIVMNDGGNQNHWIDVALQAQQLKGQQFAASGRVSPYGVGSL
                     LELKAGPWYQARVVRGQSTHFGIGQLPQADVVRVVWLNGVPQNMVQPAANLSLCEQQV
                     LTGSCPYLYAWDGERFVFLTDLLWNAPIGLQLAEGVLAPARDWEYLKLSGEKLAPEDG
                     HYALQLTEELWEAAYFDHVRMIAVDHPAEVEIYSNEKVGPPDLAQYKIHTVRQPRPVV
                     AARNHNGRDLLSDIASHDGIFAKVHDRKLRQGLVEEGYLELDLGDLARGKQITLFLTG
                     WVYPAATSINVALSQAGSLAPARPPALLVPDGNGGWNEALPFMGFPGGKTKTIAVDLT
                     DVLAEGDSRVRIATTMELYWDRIFFTVDEAPAEVRTQELDLVSADLHDRGFSRVVPDP
                     GNGPERFLYDEVLREPKWPPMAGAFTRLGDVQPLLTQRDDHLLVIGAGDEVTLRFRVP
                     DQPPPAGWKRDFLFYSVGWDKDCNLLTVLGETTEPLPFEGMPSYPWPPGEAPVLSPEY
                     QDYLRTYQTRHQSNGFWRLIQRFP"
     gene            7946..10855
                     /locus_tag="VFV87_09970"
     CDS             7946..10855
                     /locus_tag="VFV87_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX5104127.1"
                     /translation="MATNTTSDSSSAPAPTRAARKPYVPAVTPGLRKLLYVVLGLLAL
                     LGANSAYLLSVTALETFTGHVYQNYFSLLMFLAHVVLGLLLIVPFVVFGALHIRNSWR
                     RKNRRAVRVGYALFAVSLLVLITGVLLMRVLNFDLRHPTARAAIYWLHVLAPLAAAWL
                     YWLHRLAGPPIQWRVGLAYGGVVGGAVLAMIMLHSQDPRQWNVAGPTEGEVYFQPSLA
                     RTSTGNFIPARTLMMDNYCQKCHPDVHANWSQSVHRFSSFNNPAYLASVKETREVALK
                     RDGSVKASRWCAGCHDPVPFYSGAFDDPKFDVLQHPTAHAGITCTVCHAITHVNSTEG
                     NAAYTIEEPEHYPFAASNNPLLQWVNNQLVKAKPDLHKKTFLKDFHRTAEFCSTCHKV
                     SLPKELNHYKEFLRGQNHYDPYLLSGVSGHGARSFYYPPEAKTNCAQCHMPLAASSDF
                     GAKPFDDSGKLTVHNHLFPAANSGIAWLRNEPDVITAHQEFLKDVMRVDLFGVRDGGA
                     IDGELHAPLRPEVPALAPGKQYLLEAVIRTMKMGHLFTQGTVDSNEIWVDITVTSGER
                     VIGRSGGIDTEKGNEVDRWAHYVNVFLLDREGNRINRRNPQDIFTPLYNHQIPPGAAA
                     SLHYGLRVPDDVTAPITVELKLQYRKFDTEYMQFVAKNGKVKGEPIRGDTGQGPYVNE
                     LPITTLAVDRITFPVEGIAAEVANAERDIPAWQRWNDYGIGLLLKGKAELRQAEEAFQ
                     HVEELKQYHGPLNLARVYFTEGRLDEAAEALQRAAEYTDPPAPPWTLAWLSGLVSQQQ
                     PGGLEAAERNFRKVLEERTPAMIERKFDFSLDYEVRNQLGTVLFDLARQQRGEANRAE
                     RERLLKEAIAQFETTLKTDSENVAAHYNLHQLYEELSDKEKAAEHLRQHARLKPDDNA
                     TDRAVRLAREKYPAGNHAAEAVVIYPLQRPQAPELPAAEAREGEFYLSTPIPASP"
     gene            10865..>11061
                     /locus_tag="VFV87_09975"
     CDS             10865..>11061
                     /locus_tag="VFV87_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104128.1"
                     /translation="MSKAPPDAEPSQEDVQSDEIIGTALIASAAVLVIGGLVAAGVAW
                     WLFQPAPPQVGVPKDLVLPTAR"
BASE COUNT         1937 a         3746 c         3531 g         1847 t
ORIGIN      
        1 taaccgaagt gtcgctcgac cagctcgaag gaaccgtcct cgatgtcggc cccgagagcg
       61 tgcagttcga gtatgacggc gagaaaatcg acgtcccccg cgggaagctc gaggccgtcg
      121 tttattacca tccggcccgc cgcgagttct cagccccggc gtgcaagctc gtcgatgtcg
      181 gcggctcggc ctggtcgctc aagtcgctgg aactggccga ggggcaagtc cgcggcagca
      241 cggtcggcgg cgtggcgatc gatttcccgc tctccgccgt cgccaagatc gactactcgg
      301 tcggcaacgt cttctttctg gccgagctgg agcccgatac cggcgcgggc gcccccgcgg
      361 tcagcctgca gccggcatcc atggcgttca agttcggccg catcttcggt gtgcgcgcgg
      421 ggccgcccct cggcgcttcg cgcttccaga ttggcggcac ggcatacgac aatggcctgt
      481 cgctgcacag cccggcttcg atcgtttatc gcgtgcccga gggcgctcgc tggttgcggg
      541 ccgaagcggg cgtcgatgac tcggtcgtcg cccctggcga gtttgacctc gtgattcagg
      601 gcgatggcaa ggaactcttg cgtcacagct ttggccagtc cgaggaccgc aagcccctgc
      661 cgatcaatct cgacatcgcc ggcattcggc ggatcacgat cagcctcgat ccggccgacg
      721 gccaggacat cggtgaccag ctcaatctgt gcaaagcgag gatgaccaaa tgaggctgct
      781 gatccaatcc accctcgcat tcgccctgct tggcctgccg ctcacaatcc gcgctgaggt
      841 tccccagccc gtcctcgaag ccgagcaggc ccgcattgcg tcgatcgaaa aagcagtcaa
      901 gaccgcggtt gccgtcttcg ccaagggggg caatggcggc ggctcgggcg tggtgatctc
      961 gcccgacggc tatgccctga cgaacttcca cgtcgtccaa cccgccggcg accacatgaa
     1021 gtgtgcgatg gccgacggcg agctgtacga cgcggtcatc gtcggcatcg atccgaccgg
     1081 cgatgtcgcc gtgatcaaac tcctggggcg tgacgacttc ccctactcga tcatggccga
     1141 cagtaacgag gtccaggtcg gcgactggtg ctttgccgtc ggaaatccct tcctgctggc
     1201 gaccgatttc cagccgaccg tcacttacgg catcgtcagc ggaacgcacc gctaccagcc
     1261 gccggccggc acgctgctgg aatacaccga ttgcctccag accgacgcct cgatcaaccc
     1321 cggcaactcc ggcggtccgc tgttcaacgc caatggcgat cttgtaggga tcaacggccg
     1381 cggcagcttc gagaagcggg ggcgggtgaa cgtcggcgtg ggctatgcga tctcgatcaa
     1441 ccagatcaaa aacttcctgg gttgtctcaa gagcgggcgg atcgtcgatc acgcgacgct
     1501 cggcgcgacc gtcgccatga gcgacgacgg cacggtccgc gtctccaaca tcctggaatc
     1561 gtccgacgcc tatcgccgcg gactccgcta cgacgacgaa atcgtctcgt ttggcggccg
     1621 gccgatcgat acgcaaaacg ccttcaagaa cgttctggga atctatccca agggctggcg
     1681 cgtgccgctg gtctatcgcc ggggcggcga gcggcaggag attctcgtgc ggctgacggg
     1741 cgtgcacgcc gaggaggagc taaccgagct gctgcagcgg cgtcctgagc agcggccgat
     1801 gccccagccg cggcccggcg gccgtccgat gccgatgccg cagccgggcg atgatccgga
     1861 aaagcccggc gaaggcccca agcccgccga gccgcccaag cccggcgaac ccaagccgct
     1921 gccgaagctg ccgatcgagc tcggcaagaa agagccgccg atcccgccgg agattcagaa
     1981 gctgatcgtg gcccgccctg gctacgcgaa ctattacttc aacgagcaga accgtgaccg
     2041 cgtctggtcg gccttcagca agaccagcgg cgatttttcc gctgccgccg gcgagtggaa
     2101 gcttaccggc gagttggaag ggggcggcaa agtcgaggtg acgcttggcg atgaaaccag
     2161 caccggcaac ttcccgcaag gcgcggtgaa gctcgacggc gggcaggatc tcgaccagca
     2221 gcttggcccc gccggcagcg gcggcctggt tgcggcgttg cacctttggc gcacgctgct
     2281 ggtgacagga ccgcagaaat tcggcctggt ctattactac ggcacggcgc cctatcccgg
     2341 gatcgaaagc caggccgacg tgctggtcgc cacccgcaat gttgccgaag tgaacctcgt
     2401 cttcgatcct aaaagcggcc aactcgccat tctggaaatg gtcgccgacc ccgacgccga
     2461 cccgtgcgaa atccgcttcg gcgattaccg cgacgtcggc ggccggcaag tgccgcaccg
     2521 cctcgaagtt cgccacggcg atgcggcgtt cggcacgatt ctgcttagcc agatcgaagt
     2581 gggagccaaa ccgtgacgag acgaacccgc ttttgctcga tgattctcgc gctggccgcg
     2641 atcgggctgc tgggcttgcc ctatgcagct tgggcccaga cttcgttctc cgaaacgatc
     2701 gatcagacgc agcccaagat cgtcaagatc ttcggcgccg gcggcctgcg cggcctggag
     2761 ccctatcaga gcggcttttt gatttcggcc gacggccaca tcctcaccgt ctggagctac
     2821 gtgctcgaca gcgacacggt gaccgtctat ctcaacgacg gtcgcaagct ggaggcggaa
     2881 gtcgtcggca tggacccgcg gacggaaatt gccgtcctca agatcgatgc ccagaacctg
     2941 ccgcatttca acctggacga agcggtcgcg ctgaatgccg gcgcgcgcgt gctggccttc
     3001 agcaatttgt acggcgtggc cctcggcaac gagccggcca gcgtgctgca cgggatcgtt
     3061 tcggccaaga ccgatctggc ggctcgccgc ggggccttcg agacgaacta ccgcgggcaa
     3121 gcctacgtgc tggacgccat gaccaacaac cccggcgcag ccggcggcgc gctgaccgac
     3181 cgccgcggcc ggctggcggg cctgctcggc aaggagctgc gcagctcgct cagcaatatc
     3241 tggctgaact acgccattcc gatctccgag ctggcgacga ccgccgacga aatcatcgcc
     3301 ggcaaattcc gcccggcccc tcgcgacgaa ggcgaaaaga agcccaagga gccgcacacg
     3361 ctggccctgc tcggcatcaa tctggtgacc gattttctga ctaagacgcc gccgtttatc
     3421 gagagcgtca agcccggctc ccccgcggct aaggccggcc tgcggaccga cgacctgatc
     3481 ctgttcgtca acagccgcgt cgtccaatcg tgtaagctgc tcgtggatga gctgtcttac
     3541 atcgatcgtc tggaccccgt gcggctgacg gtgcagcgcg gccaggatct gctggaagtg
     3601 gaactgcagc cggtcccgta ggcagcgcct gcgatcttag ccgcgcccat tcttgctcac
     3661 tcgctatgcc ccaccgctgg agcgcgccgc gcacgattgc cctgatcttg atcgccctgg
     3721 cgtgcgtcgc cgccgccatc tatttcgcca gccgcttcgg cggcggcccc tcggctgacg
     3781 aggtgcggca gcttgtggaa gagaagaacg tcggcctggg ctacctcgaa aatcagacgc
     3841 tcgacgatgc cgtcagcagc ttcgagaaga ttcgcgcgaa gctgcccgcc gatccgcttt
     3901 cctggcgcaa tctggcgatt gcccgcgtgc tcgcgctggg ggacgacggc gccgatgcca
     3961 ctctggagaa gctggcagcc gcaacttcgg ccctgaagga ggtctcgcgg ctcgagggtg
     4021 caagcgctcc ctaccactgg ctatcgctgc aaacggcgct ggccgcggag gactttgcgg
     4081 cggcccagac gcatctgaat gcgatccagg ctgaggaatc tggcgatgcc gcggcctggt
     4141 tcgcgaagtt cagcgcgctg gagcgccaaa cgggtgagaa gctcgaccct ccggctctcc
     4201 aggctctgga gaaggcctgc cagctgcgac ccgagaatgc ctggctgcgc gttgaatggc
     4261 tgcgggccgt aggcacggcg ttgctgcagc ttccggagga cgcgggccgc gcggatgccg
     4321 ccgcaggggc ctggggcgac ctggcggctc agctcgacca gggccaacgg gccattgagc
     4381 cgttcgcgcc gctgattcag acgcacacga aagttgatgc gctggccctc ctgtcggaag
     4441 cgaagcaggc ggtcgccgac cgccagtggc gcgaggccgg ccagcggatg ctcacgctgg
     4501 ccaatgtcct cgtgccgcac gccgcgctgg accggatgca agtccgccgg catccgctgg
     4561 agttcgtagc taccgagttc cagccccaga ttatgcgcgg catgcccccc acggaggagg
     4621 aaaagccgat tgccgtctca tttcgcccgg gcggcgaggg gagcgagtcg ctatctgcgg
     4681 cgaccaaggg cgccgtggac ttcgccctgg ccgatttcga tctcgatggc cggctggatg
     4741 tcgtagcgct cttgccggac aagatcgtcg tgctcagtcg cggcatcgag gggcaagatt
     4801 ggcaaacgat cgcctcggcg gaagtctcgg gcgctgagca gctgctcgtt caggacctcg
     4861 attccgactt cgacgaaacc gtgctggccg tccacggcgg cggccatgtg gagggccaaa
     4921 aggagaagga caaacccgca ggggcaccct gcccgacggc tgacgtggat atcgtggcct
     4981 atggccagag cggcgtgatg ttgctggaga accggttcga cccggcaacg cgctctcgca
     5041 cgctggtggc tgtgccaggg gagaaactcc cggcggcact catggacgta gccaccctcg
     5101 ccgccgccga cctggaaggc gacggcgacc tcgatctggt cctcgccgct ggcgacacgc
     5161 tgcaccgctg gatgaacggc ggcgagtgga cgtttctggc cgcagccagc gatgaacgac
     5221 ccggcgtcac acagatcctg ccgctcgact tcgaccgcga catcgatatc gacctgttga
     5281 ttgcctcgcc aaacgaagcc ggctggctgg agaacgtgcg gcacggcggc ttccgctggc
     5341 ggccttttgc cagagatttt cccgacctcg ccacggctac cgccttggag agcgtggatt
     5401 tcgatggaac cgcttcgtgg gacctcgtgg ccgcgaccga ccagggcctg cgactggtca
     5461 ccacgcgcac gccgcagccg ggcgacattc ggctcaagga gtccctctcc attgcgagcg
     5521 ccaaaccgga tcgcctggcc gcgttcgatt acgacaacga tggccacgac gatctgctgt
     5581 cgtggaccaa ggagcagacg acggttgcgc gctgcgagcc cgcttcagga gcagcgccac
     5641 gtacttggaa cgtcgaaacg ccggcctatc gcagcgccgg cccagccaag tccgccgcgc
     5701 agggcgatct cgataacgac ggcgatctgg acatcgccgt tctggatgat aggggcgtgt
     5761 ccatcgtaat gaacgacggc ggtaaccaga accattggat cgatgtcgct ctgcaggccc
     5821 agcagctcaa gggtcagcag ttcgcagcca gcgggcgcgt ctcgccctac ggcgtcggca
     5881 gccttctgga gctgaaggcc ggcccgtggt atcaggcgcg cgtcgtgcgc ggccaatcga
     5941 cccacttcgg tattggccag ttgccgcagg ccgatgtggt ccgcgtcgtc tggctgaacg
     6001 gcgtgccgca gaacatggtc cagcccgccg cgaacctttc gctctgcgag cagcaggtgc
     6061 tgaccggctc gtgcccctac ttgtatgcct gggacggcga gcgattcgtg ttcctgaccg
     6121 atctcttatg gaatgcgccg atcggcctgc agctcgccga aggcgtgctg gccccggccc
     6181 gcgactggga atacctcaag ctctccggcg agaagctcgc gccagaggac gggcattacg
     6241 ctctccagct caccgaggag ctgtgggaag ccgcctattt cgaccacgtg cggatgatcg
     6301 ccgtcgatca cccggctgag gtcgagatct actcgaacga gaaagtcggc ccgcccgacc
     6361 tggcccagta caagatccac accgtgcgtc agccgcggcc ggtcgttgcc gctcggaacc
     6421 acaacgggcg cgacctcctg agcgacatcg ccagccacga cggcatcttt gccaaggtcc
     6481 acgaccgcaa gttgcggcag ggtcttgtcg aagagggtta cctggaactc gacctgggcg
     6541 acctcgcgag gggcaagcag atcacgctgt tcctcaccgg ctgggtctat ccggcggcga
     6601 cgtcgatcaa cgtcgccctc tcgcaggccg gcagccttgc gcccgccagg ccgccggcgc
     6661 tcctcgttcc ggatggcaat ggcggctgga atgaggcgct ccccttcatg ggcttccccg
     6721 gcggcaagac gaagacgatc gccgtcgatc tcacggacgt cctcgccgaa ggcgacagcc
     6781 gcgtgcggat cgccacgacg atggagctct actgggaccg tatcttcttc acggtcgatg
     6841 aagccccggc ggaagtccgc acgcaggagt tggacctggt cagcgccgat ctgcacgacc
     6901 gcggcttctc ccgggtcgtt cccgatcccg gcaacggtcc ggagcgattc ctctacgacg
     6961 aagtcctccg cgagcccaag tggccgccga tggccggcgc gttcacgcgg ctgggcgacg
     7021 tccagccgct gctgacccag cgcgacgacc acctgctcgt catcggcgcc ggcgacgaag
     7081 tgacgctccg cttccgcgtc cccgatcagc cgccgcccgc cggctggaag cgcgatttcc
     7141 tgttctacag cgtcggctgg gacaaggatt gcaacctgct caccgtcctg ggcgagacca
     7201 ccgaaccctt gcccttcgag ggcatgccca gctatccctg gccgccgggc gaagcaccgg
     7261 ttctgtcccc cgaatatcag gattacctgc ggacatatca aacccgccat caatccaacg
     7321 gcttctggcg gctgatccag cggtttcctt gaacggccca agccagcccg ctatgatcga
     7381 atgatcgacg ctcatcttgt ggccggatgc ggggatgctg gggtgctgcg ttcctatcct
     7441 caaacgtttg tgctattgct ggccgggctg ctctgcccgc tggcgatgag cagcgcgcgt
     7501 ggtgcctgcg gcgattggct cgccgaaccg cacgctccat cacaacgcgc ggctggcgac
     7561 ggtcgttctc ccagcgacct tccggcgccc gagctcccct gcagcgggcc cgcgtgcggc
     7621 aagtcgccgc tggcgcccct ccctgttcca ctcgcccagg accgtgcggc ggaaaaggct
     7681 ggaatctcaa cttccgttgg ctgcgcatct ggcccttcgc gcagcgatgc attcgctcgc
     7741 ccgcgcgatg atcgccatct cgccgaaggc ttctatccgg ggctgcttcg cccgccgcgt
     7801 tgtgtcgttt gcttgtgacg ccaacgcgcg tcgcttgaca aacttccagc gcgtgccgct
     7861 ccatcgcaaa gcacgaccgg gcggcgatcc ctccgcgctg caacgcgcag aaccagcccg
     7921 cggcagtatt ctggtgaggc gaaccatggc aaccaacaca acgtctgact cgtcctccgc
     7981 tcctgctccc acgcgcgcgg cgcgcaagcc gtatgtcccg gcggtcacac cggggctgcg
     8041 gaagctgctg tatgtggtgc tgggcttgct ggcgctcttg ggggcgaatt cggcctactt
     8101 gctgagcgtg accgcgctgg agacctttac gggacacgtc tatcagaatt acttttcgct
     8161 gttgatgttc ctggcgcacg tcgtgctggg attgctcttg atcgtgccgt tcgtcgtgtt
     8221 cggcgcgctg cacatccgca actcgtggcg gcggaagaac cgccgggcgg tgcgcgtggg
     8281 ctatgcgctg tttgccgtgt cgctgctcgt gctgatcacc ggcgtgctgc tgatgcgcgt
     8341 gctgaatttc gacctgcggc atccgacggc ccgcgcagcc atttattggc tgcacgtcct
     8401 cgcgccgctc gccgcggcgt ggctctactg gctacatcgg ctggcggggc cgccgatcca
     8461 gtggcgcgtc gggttggctt acggcggcgt ggtcggcggg gccgtgctgg cgatgatcat
     8521 gctccactcg caggatccgc ggcagtggaa cgtcgccggg ccgacggaag gcgaggtcta
     8581 tttccagccg tcgctggccc gcacttcgac cggcaacttc attccggcca ggacgctgat
     8641 gatggacaac tactgccaga agtgccatcc ggacgtgcac gccaactggt cgcagagcgt
     8701 ccaccgcttc agctcgttca ataaccccgc gtacctggcc agcgtcaagg agacccgcga
     8761 agtcgccctg aagcgcgacg gcagcgtgaa ggcttcccgc tggtgcgccg gctgccacga
     8821 ccccgtgccg ttctatagcg gggcgttcga cgacccgaag ttcgacgtgt tgcagcatcc
     8881 cacggcccac gccggcatca cttgcacggt ttgccatgcc atcacgcacg tcaacagcac
     8941 cgagggcaac gcggcctaca cgatcgaaga gcctgagcac tacccgttcg ccgccagcaa
     9001 taatccgctc ttgcagtggg tcaacaacca gctcgtaaag gccaagcccg acttgcacaa
     9061 gaagacgttc ctcaaggatt ttcacaggac tgcggagttc tgctcgacct gccacaaggt
     9121 gagcctgccc aaagagctga accactacaa ggaattcctc cgcgggcaga accattacga
     9181 tccgtacctg ctctccggcg tttccggaca cggcgcgcgg agtttctact atccgccgga
     9241 ggctaagacc aactgcgccc agtgccacat gccgctcgcg gcgtcgagcg atttcggcgc
     9301 gaagcccttt gacgacagcg gcaagctgac ggtccacaat cacctgttcc ccgcggcaaa
     9361 ttccggcatc gcctggctcc gcaacgagcc ggacgtcatc accgcccatc aggaattcct
     9421 gaaggacgtg atgcgggtcg atctgttcgg cgtccgcgat ggaggcgcga tcgacggcga
     9481 gctgcacgca ccgctgcggc cggaggtgcc ggcgctggcg cccggcaagc aatacctgct
     9541 ggaggcggtt atccgcacga tgaagatggg gcatctcttc acgcagggga cggtcgattc
     9601 caacgagatc tgggtcgata tcaccgtgac cagcggcgag cgggtgattg gccgcagcgg
     9661 cggcatcgat acggagaagg ggaacgaggt cgatcgctgg gcccactacg tcaacgtctt
     9721 cctgctcgac cgcgaaggga accgcatcaa ccgccgcaat ccgcaggata ttttcacgcc
     9781 gctctataac caccagatcc cgccgggcgc ggcggccagc ctgcactacg gcctccgcgt
     9841 gccggacgac gtgaccgcgc cgatcacggt cgagctcaag ctgcagtacc gcaagttcga
     9901 caccgagtac atgcagttcg tcgccaagaa cggcaaagtg aagggcgagc cgatccgcgg
     9961 cgatacaggc caaggtccgt acgtaaacga gctgccgatc accacgctgg ccgtcgatcg
    10021 catcacgttc ccggtcgaag ggatcgcggc ggaggtcgcc aacgccgagc gcgacatccc
    10081 cgcctggcag cggtggaacg actacggaat cggcctgttg ctcaagggca aagccgagct
    10141 gcggcaggcg gaggaagcgt tccagcacgt cgaagagctc aagcagtatc acgggccgct
    10201 gaatctggcc cgcgtctatt tcaccgaagg gcggctggac gaagcggctg aggccctgca
    10261 gcgggccgcg gaatacaccg atccgccggc gcccccctgg acgctcgcct ggctcagcgg
    10321 cctggtcagc cagcaacagc ccggcggcct ggaggcggcc gagcggaact tccgcaaggt
    10381 gctggaggag cgcacgcctg caatgatcga gcggaagttc gatttcagcc tcgactacga
    10441 ggtccgcaac cagctcggca cggtcctctt cgacctggcc cggcaacagc gcggcgaggc
    10501 gaaccgggcc gagcgcgagc gtctgctcaa ggaggcgatt gcccagttcg agacgacgct
    10561 gaaaaccgat tcggaaaacg tcgcggccca ctacaacctg caccagctct acgaagagct
    10621 gagcgacaag gaaaaggcgg ccgaacactt gcggcagcac gcacgcctca agcccgacga
    10681 caacgcgacc gaccgcgccg tgcgcctggc ccgcgagaag taccccgccg gcaaccacgc
    10741 cgccgaagcg gtcgtgatct atccattgca gcggccacaa gcgccggagc tgcccgctgc
    10801 cgaagcgcgc gaaggcgagt tctacctgtc cacacccatc cccgcttccc cgtaagagcc
    10861 acgcatgtcc aaagcacctc ccgatgccga accttcccag gaagacgtcc agtccgacga
    10921 aatcatcggc acggcgctga tcgcttcggc ggcggtgctc gtgatcggcg ggctcgtggc
    10981 cgcgggtgtg gcctggtggc tctttcagcc ggcgccgccg caggtcggcg ttcctaagga
    11041 cctggtcctt cccacggcgc g
//
LOCUS       DASUGB010000520         5025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_57766,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000520 DASUGB010000000
VERSION     DASUGB010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57766"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFV87_09980"
     CDS             complement(<1..518)
                     /locus_tag="VFV87_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5104129.1"
                     /translation="MAASKHILIVEDERHLAAGIKFNLEAEGYRVTLAADGPAAIAAF
                     DEDAAAIDLMILDVMLPGMSGYQVCEAIRERGSDLPVLILSARTLSEDRTRGFDVGAN
                     QYLSKPFDLDELLSRVRNLLTHHPRRHETPRAVPSKLTTYQFAAAEINFPEYEVAVAG
                     KPVQLTKREWELL"
     gene            703..3606
                     /locus_tag="VFV87_09985"
     CDS             703..3606
                     /locus_tag="VFV87_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5104130.1"
                     /translation="MKLVSLCSAERIRIACALAAAAACLSAQAPAAEPAPPVISAEQE
                     RFFEEKVRPVLAAKCLECHGEKKQESGLRLDSRQGVLGGGDSGEPAVVPHEPDRSLLV
                     KAINHVGDYHMPPTTKLPDEQIAALTEWVKIGVPWPGGDSVPAARLSAADRAEHDQQT
                     HWAYQPVQRPAIGPVRDASWPVSGLDALILSKLDIAGLTPSPAADRRTLIRRLTYDLS
                     GLPPTPDEVDAFIADAAPDAYERLADRLLASPHFGERWGRHWLDVARYADTKGYAFAK
                     ERRYPYSYTYRDYVIRAFNRDLPYDQFILQQLAAERLPGASEDVASLAALGFLTVGRK
                     FNNRPDDIDDQIDAVTRGFLGMTVGCARCHDHKYDAIPTEDYYSLYGVFASCNEPEEL
                     PLIGEAAEGEEYRQFEEELAKLRGELDKFAAAKHAEFLDQSRRQAADYLARVAAGSTN
                     TLLNRLPFLSLDPKELRSRMIRRWQQFIDQRAKPDHAALGLWNDLLKVPTDGFAKKVT
                     PVLEKWQALPAGTAAGQCNPLVQGAFKADVPATRMDVPRIYGKLLTDAYTAWQAAGAN
                     ADALGKLPADQRQLAELLLGPGAPTDISKDDVSDYLSRADRNQHSELKKKVESFQATS
                     PAAPPRAMVLKDFPQPGDSRVLIRGKPDRPGNVVPRQFLLVLSETGRQPFRDGSGRLE
                     LARAIVDPQNPLTRRVIVNRLWMHHFGEPLVNSPSDFGLRCEQPVQPEVLDYLASLLL
                     ERGWSLKDMHREMVLSSTYRQTSNAEFGMRNAESQTQLTANPHSALRNPQSVDPENRL
                     LWRMNRRRLELESVRDTLLSLAGRLDESLYGRPVELTSAPFTRRRAVYGFIDRQDLPN
                     MFRVFDIASPDQSSPRRPRTTVPQQALFLMNSPFAVEQAQALAARLPADLSDDAQRMS
                     ALYRLVLQREPTAAEAAIGREFLAAPNPPGSRLTPLEQYAQLLLLTSEVMYID"
     gene            3643..3954
                     /locus_tag="VFV87_09990"
     CDS             3643..3954
                     /locus_tag="VFV87_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX5104131.1"
                     /translation="MPQVSVEHIVIDGNGIARLAGRRTKVSQIAMDKIAFGWDAEQIQ
                     RQYPHLGLSEIHAALAYYYDHQSEIDRQIAESKAKAESIRSQTENHALADQVRQRAVQ
                     P"
     gene            3951..4301
                     /locus_tag="VFV87_09995"
     CDS             3951..4301
                     /locus_tag="VFV87_09995"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5104132.1"
                     /translation="MSLRLFMDEHVKSAVTDGLRQRGYDVLTAQEDGSDGLPDPEQLD
                     RATFLGRVFVSQDEDFLTEGARRQAAGVDFAGIIYGHQLDLTIGQMINELEMVVYCLV
                     EAEIRGQIYYIPLR"
     gene            4311..>5025
                     /locus_tag="VFV87_10000"
     CDS             4311..>5025
                     /locus_tag="VFV87_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104133.1"
                     /translation="MSGFSQQIPTALTRRELLRRSGMGLGFLGLAGVLADGGLLNLPT
                     EAADDAGYTNPLAERPPHFPAKAKRVIHLVMNGGPSHVDTFDPKPALEKYAGQNLPTE
                     NLRTERKTGAAFPSPFKFQQYGESGIPVSELFAHTAQHIDDICVVRSMHADVPNHEPS
                     LLLMNCGESRLVRPSMGSWLTYGLGTENLNLPGFIAMCPGGFPIVETQNWQAAFLPGV
                     YQGTYVNTQNTDIEKLVDNV"
BASE COUNT          915 a         1586 c         1613 g          911 t
ORIGIN      
        1 agcagctccc attcgcgctt cgtgagctgc acgggcttgc cggcgactgc gacttcatac
       61 tctgggaagt tgatttcggc ggcggcgaac tgatacgttg tcagcttgct cggcacagcc
      121 cgcggcgttt cgtgccgacg cgggtgatgc gtcagcaggt tccgcactcg gctgagcaat
      181 tcgtcgaggt cgaacggctt gctgaggtac tggttggcgc cgacatcgaa gccgcgggtg
      241 cggtcttcgg agagggtgcg ggccgagaga atcaacaccg gcaggtcgct gccgcgttca
      301 cggatcgcct cacagacctg atagccgctc atgccgggga gcatgacgtc gaggatcatc
      361 aggtcgatgg cggcggcgtc ttcgtcgaag gccgcaatcg ccgccgggcc gtcggccgcc
      421 agcgtaacgc gatagccctc ggcttccagg ttgaatttga tcccggcggc caaatgtcgt
      481 tcgtcttcga cgatcaggat gtgcttggag gcggccatgg ggggaggtca gttgtccctt
      541 gtcatttatc ctttgtaact tgcgctgcgg cgaaagggcg gtcaagaata gccgcttcca
      601 atggaggttt gggttgccgg ccaggcggtt ttgatttcta ctggaggagg gcgatgttgt
      661 cgtcctcccg ccacagaacc cgcctgagcc ttctggtgct cgatgaagct cgtttcttta
      721 tgctccgcgg agcggattcg gatcgcttgc gccctggcgg ctgccgcggc ttgcctgtcg
      781 gcacaagcgc cggctgcgga gcccgctccg cccgtgatct cggccgagca ggagcgtttt
      841 tttgaagaga aggtccggcc ggtgctggcg gcaaagtgcc tggaatgcca cggcgagaaa
      901 aagcaggagt cgggtttgcg gttggattcg cggcagggag tgctaggcgg cggcgacagc
      961 ggtgagccgg cggtcgtgcc gcatgaaccg gatcgcagcc tgctggtgaa ggcgatcaac
     1021 cacgtcggcg actatcacat gccgccgaca acgaagctgc cggacgagca gattgcggcg
     1081 ctgaccgagt gggtcaagat cggtgtcccg tggcccggtg gcgattcggt tccggcggca
     1141 aggctctcgg ccgccgaccg cgcggaacac gatcagcaaa cccactgggc ctatcagccc
     1201 gtgcagcgcc cggcgattgg gcccgtgcgg gatgcctcgt ggccggtcag cgggctggac
     1261 gcgctcatct tatcgaaact cgatattgcg ggcctgacgc catcgcccgc tgccgatcgc
     1321 cgtacgctca tccgccggct gacgtatgac ctgagcggtc tgccgccgac gccggacgaa
     1381 gtggatgcat tcatcgccga tgcagcgccc gatgcctacg agcgcctggc ggaccgcctg
     1441 ctggcgagcc cgcacttcgg cgaacgctgg ggccggcact ggctcgatgt ggctcgctac
     1501 gccgacacga agggctacgc cttcgcgaag gagcggcgct atccgtattc gtacacgtac
     1561 cgcgattacg tcatccgcgc cttcaaccgc gacttgcctt acgaccagtt catcctgcag
     1621 cagcttgccg ccgagcgctt gcccggcgcg agcgaggatg tcgcttcgct ggcggcgctc
     1681 ggcttcctga cggtcggccg caagttcaac aaccggcccg acgacatcga cgaccagatc
     1741 gacgccgtga cgcggggatt tctgggaatg accgttgggt gtgctcgctg ccacgatcac
     1801 aagtacgacg cgattcccac cgaggactac tattcgctct acggcgtgtt cgcgagctgc
     1861 aatgagcccg aagagttgcc gctgatcggc gaggcggcgg aaggcgagga gtatcgccag
     1921 ttcgaggagg agctagcgaa gctccgcggc gagctggata agtttgctgc cgcaaagcac
     1981 gccgagttcc tcgatcaatc gcggcggcag gcggctgatt atttggcccg cgtcgccgcg
     2041 ggatcaacaa atacgctgct caatcggctg ccgttcttgt ccctcgatcc gaaggaactg
     2101 cggtcgcgga tgatccgccg ctggcagcag tttatcgatc agcgggccaa gcccgaccat
     2161 gcggcgctgg gactgtggaa cgacctgctg aaagttccga ccgacggctt tgcgaagaag
     2221 gtcacgccgg tgttggaaaa atggcaagcg ttgccggccg gaacggctgc cggccagtgc
     2281 aacccgctcg tccaaggcgc gttcaaggcc gatgtgccgg ctacgcgaat ggacgtgccg
     2341 cgaatctatg gcaagctgct gacggatgcc tataccgcgt ggcaagccgc cggcgcgaat
     2401 gccgacgcgc tgggcaagct gcccgcagac cagcgccaac tcgcggagtt gctgctcgga
     2461 ccgggcgcgc cgaccgacat cagcaaggac gatgtatccg actacctgag ccgggccgat
     2521 cgcaatcagc actccgagct caagaagaag gtcgagtcgt tccaggccac ctcgcccgcc
     2581 gcgccgccgc gggcgatggt cctcaaggat ttcccgcagc cgggcgattc gcgcgtgctg
     2641 atccgcggca agccagaccg gcctgggaat gtcgttccgc ggcaattcct gctggtgctt
     2701 tctgaaacgg ggcgtcagcc gttccgcgac ggcagcggac ggttggagct ggcccgggcg
     2761 atcgtcgatc cgcagaaccc gctcacgcgg cgggtgatcg tcaatcggct gtggatgcac
     2821 cacttcggcg agccgctggt caattcgccc agcgatttcg gcctccggtg cgagcagccc
     2881 gtgcagccgg aggtgctcga ctatctggcg tcgctcttgc ttgagcgcgg ctggtcgctg
     2941 aaggacatgc accgcgaaat ggtgctgtcg agcacgtatc ggcagacttc aaatgcggag
     3001 ttcggaatgc ggaatgccga atcgcagaca caattgaccg ccaatccgca ctccgcactc
     3061 cgcaatccgc aatcggtcga tccagagaac cgcctcctgt ggcggatgaa ccgccgccgc
     3121 ctggagttgg aatcggtccg cgacacactg ctgtcgctgg caggtcggct ggatgaatcg
     3181 ctttacggcc ggccggtcga gctgacgtcg gcgccgttca ctcgccgccg cgcggtctat
     3241 ggctttatcg accggcagga cttgccgaac atgttccgcg tattcgatat cgccagcccc
     3301 gatcaaagca gcccccgccg cccgcgcacg accgtcccgc agcaggccct cttcttgatg
     3361 aattcgccgt tcgcggtcga gcaagcccag gctctcgccg ctcgcctgcc ggctgactta
     3421 agcgacgacg cgcagcggat gtccgccctc taccgcctcg tcctccagcg cgagcccacc
     3481 gccgccgaag cggccatcgg ccgcgagttc ctcgccgccc ccaatccacc cggcagccgc
     3541 ctcacccccc tggagcaata cgcccaattg ctgctgctga cgagtgaggt gatgtatatt
     3601 gactgacggg gattaacaat gagcacacca accacttcga ccatgccgca ggtttccgtc
     3661 gagcacattg tgatcgatgg taatgggatc gcgcgccttg cgggccggcg gacgaaggtg
     3721 tctcaaatcg cgatggacaa aatcgcgttc ggctgggatg cagagcagat tcagcggcag
     3781 tacccgcatt tgggattgtc cgaaatccat gccgccctgg cctactacta cgaccaccaa
     3841 agcgagatcg atcgacaaat cgcagaatct aaagcgaagg ccgaatcgat tcgctcgcaa
     3901 acagaaaatc acgcacttgc ggatcaggtc cgccagcgcg cggtgcagcc gtgagcttgc
     3961 gattgttcat ggatgagcac gtcaagtcgg cggtgaccga cggattgcga caacgcgggt
     4021 atgacgtgtt gaccgcacag gaagatggca gcgacgggct tcccgatccg gaacaattgg
     4081 accgggcgac gttcctcgga cgcgtcttcg ttagtcaaga cgaggatttt ctgacggaag
     4141 gcgcccggcg acaagcggct ggagtcgatt tcgctggcat catttatgga caccagctgg
     4201 acctgacgat tggccaaatg atcaatgagc tggagatggt ggtgtattgc ctggtcgagg
     4261 ccgagattcg cggccagatt tattacattc cgttgcggta aatcctttcg atgagcggat
     4321 tcagtcaaca gatccctacg gccctcaccc gccgcgagct cctccgccgc tccggcatgg
     4381 gcctggggtt cctcggcctg gcgggcgtgc tggccgatgg cggtttgctg aatttgccca
     4441 cggaagcggc ggatgacgcg ggatacacca atccgctggc cgagcgcccg ccgcattttc
     4501 cggcgaaggc gaagcgggtc atccacctgg tcatgaacgg tgggccgagc cacgtcgata
     4561 cgttcgatcc caagccggcg ctcgaaaaat acgccggcca gaacctcccc accgagaacc
     4621 tgcgcaccga gcgcaaaacc ggcgcggcct tcccctcgcc gttcaagttc cagcaatacg
     4681 gcgaaagcgg catcccggtc agcgagttgt tcgcgcatac cgcgcagcat atcgacgaca
     4741 tctgcgtcgt ccgcagcatg cacgccgacg tgcccaacca cgagccgtcg ctcttgctca
     4801 tgaactgcgg cgaatcgcgc ctggtgcggc cgagtatggg gtcgtggctg acctatggtc
     4861 tgggcaccga gaacctcaac ctgccgggct ttatcgccat gtgccccggc gggtttccga
     4921 ttgtcgagac gcagaactgg caagcggcgt tcctgccggg cgtctatcaa ggcacgtacg
     4981 tcaacacgca aaacaccgac atcgagaagc tcgtcgataa cgtcc
//
LOCUS       DASUGB010000521         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1726398, whole genome shotgun sequence.
ACCESSION   DASUGB010000521 DASUGB010000000
VERSION     DASUGB010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726398"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..2235
                     /locus_tag="VFV87_10005"
     CDS             259..2235
                     /locus_tag="VFV87_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX5104134.1"
                     /translation="MCLPLFLSLALTGADAAEDLQCLKPKPGEPAASTLFYASLQQQA
                     YAALDARLAEYEKLKTPDDIASYQQRAREFFLQQLGGFPERTPLHPQVVGKIEADGYR
                     IEKVIFESQPHHHVTASLYLPEASGPVPCVLVSSGHSRTAKAAEYNQRIAITLARHGI
                     AGLAYDPIGQGERSQILDEEGQPEFSGTTTEHFLVGVGSILVGRNTARYRVWDAIRAI
                     DYVCSRPEIDKERIGFTGCSGGGTLTSYTMALDDRVVCAAPACYLTTFHKLLETIGPQ
                     DAEQNIFGQVKFGLDQPDYVLLRAPRPTLISSSTGDFFDIGGSWDNYRQAKRVFARLG
                     YPERVDLVEAEGGHGVHPENLIAIVRWMRRWLLGKDDAVTIEPITPRSVEELHCTDKG
                     QVLLLPGEKSVFDLNAEYEKTLAEKRREFWKKTPKAEALEAVREQIGIRPIADLPAPK
                     MDEAGRVDRDGYHIDKFVLHTGSGVPLPGLTYHPARPVDDAYLYLHADGKAADGAPGG
                     PIEKLVQAGNVVVAIDLRGTGETAGGKPDALLGQWKSYYLAYLLGQSLVGLHTEDTLA
                     AGHFVANYKTKTPRKVHLIAVGSACIPALHSAALLPDQFASVTLRDYPDSWSTIVGHS
                     VPAGRLTTAVHGALATYDLTDLRDSLGNRVRIEK"
     gene            complement(2592..3170)
                     /locus_tag="VFV87_10010"
     CDS             complement(2592..3170)
                     /locus_tag="VFV87_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104135.1"
                     /translation="MTAYETYETKIHCLLLAGLTLLAGCIPSLNAVYTEETLDFDPAL
                     VGTWQQPGAKARWEFSKLDDKSYRLIYTDEEGQQGRFIGRLAKLDGESFLDLFPEDIE
                     MDAVGFYKFHLVPIHTIYRLQRPEGKLKLSAIDFQWLDKYLNDHPGEIQYATFSGRKL
                     ITAPTASVQTFVLAHKDQFTGNFELQKLEANN"
     gene            complement(3204..3503)
                     /locus_tag="VFV87_10015"
     CDS             complement(3204..3503)
                     /locus_tag="VFV87_10015"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5104136.1"
                     /translation="MTRTISNTSHLEPARWADEDLTHRIRLFLQSLQLAVAKRIEVEV
                     EQGVVTITGRVRSRYERQLALSCVRRVAGVRQVEDRMVVAENRPFARRESLLVDV"
BASE COUNT          729 a         1209 c         1146 g          696 t
ORIGIN      
        1 aaagtggtgc gtgaatcgga ccgtaggcat ctcgcgccgc gagatgcgat ggaagtggcc
       61 ctgcgcagcg gccctcgcgc atggaggatc gttcgctact cgcggagcga gtagcctacg
      121 gttgagttgc caacatagcc gcgggccgtc gggatgccaa gttctgcccc gcctagcggc
      181 ggtgcgtgat tcataatggc cgcgaacgta caatcgaggc acgacaactg actctcgaat
      241 cgaagttagg agctgcccat gtgcttgccc ctgttcctct cgctcgccct gaccggcgcc
      301 gatgctgcgg aagatttgca gtgcctgaag cccaagccgg gcgagccggc ggcgagcacg
      361 ctgttctatg cgagtctgca gcagcaggcg tatgcagccc tcgatgcccg actggcggag
      421 tatgagaagc tgaagacgcc ggacgacatt gccagctatc aacagcgggc gcgcgagttc
      481 ttcctccaac aactcggcgg ctttccagag cgaacgccgc tccatccgca ggtcgtcggc
      541 aagatcgaag ccgacggcta ccgcatcgag aaggtcattt tcgagagcca gccacatcac
      601 cacgtgaccg cgtcgctgta tctgcccgaa gcgagcgggc cggtcccatg cgttctcgtc
      661 tccagcggcc acagccgcac ggcgaaggcg gcggaataca accagcggat tgcgatcacg
      721 ctggcccgcc acggcatcgc gggcctggcc tacgatccga tcggccaggg cgagcgatcg
      781 caaattctcg atgaggaagg ccaaccggag tttagcggca cgacgaccga gcacttcctg
      841 gtcggcgtcg gctcgatcct cgtcggccgc aatacggccc gctaccgcgt gtgggatgcg
      901 atccgggcga tcgactacgt ctgcagccgg ccggagatcg acaaggagcg gatcggcttc
      961 acgggatgct cgggtggcgg cacgctgacg agctacacga tggccctcga cgaccgcgtc
     1021 gtttgcgccg ccccggcttg ctacctgacg accttccaca agctgcttga aacgatcggc
     1081 ccgcaggacg ccgaacagaa cattttcggg caggtgaagt tcggcctcga tcagccggac
     1141 tacgtgctgc tgcgagcccc gcggccgacc ttgatcagct cctcgaccgg tgatttcttc
     1201 gacatcgggg gaagctggga caattaccgc caggccaagc gggtttttgc ccgcctgggc
     1261 tatcccgagc gggtcgatct ggtcgaagcc gaagggggcc acggcgtgca tccggagaac
     1321 ctcattgcca tcgtgcgctg gatgcggcgc tggctgctcg gcaaggacga cgcggtcacg
     1381 atcgagccga tcacgccgcg cagcgtggaa gaattgcact gcacggacaa aggccaggtc
     1441 ctgctgttgc cgggcgagaa gtcagttttt gatctgaatg ccgaatatga gaaaacgctc
     1501 gccgaaaaac gacgcgaatt ctggaagaag acgcccaaag ccgaggctct cgaagcggtc
     1561 cgagagcaga ttggcatccg ccccatcgcc gatctccccg caccgaagat ggacgaagcc
     1621 ggccgcgtcg atcgcgatgg ctaccacatc gacaagttcg tcctgcacac gggttccggc
     1681 gtcccgctgc cggggttgac ctatcatccc gccaggccgg tcgatgacgc ctatctctat
     1741 ttgcacgccg atggcaaggc ggctgatggc gcgccaggcg ggccgatcga gaagctcgtg
     1801 caagcaggca acgtcgtcgt cgccatcgac ctccgcggca cgggcgaaac ggccggcggc
     1861 aagcccgacg cgctcctcgg ccagtggaag agctattacc tcgcctacct gcttggccaa
     1921 tcactcgtcg gcctgcacac cgaagacacg ctcgccgccg gccacttcgt cgccaactac
     1981 aaaacgaaga ccccccgcaa agtccacctc atcgccgtcg gctctgcctg cattcctgcc
     2041 ctccactccg ccgccctcct cccggatcag tttgcctccg tcacgctacg cgactatccg
     2101 gactcctggt cgacgatcgt tggtcattct gtccctgctg gaaggttgac gaccgctgtt
     2161 cacggggctc tcgccactta cgacctgacc gacttgcggg actcgttagg caaccgcgtc
     2221 cgaattgaaa agtgacgtca agaaatacaa atagccgcgt tgcggctgac gaacgtcatt
     2281 ctccctaacc ccccgagcct cgcgccttgc ttgccgctcg gaattccccg aatgcaggat
     2341 gcgggacgct gcgcgtcaaa ccctagcagc gccactcgcc acgtaaaccg aaagcggcat
     2401 gtcacgcgag gctcccttcc gccacggatg gcaaaccagt tccacacctc ctttcgagaa
     2461 gctgcgattg aagccggcgg gtgattggcc cccgcgcccc tccggagcgt ccggcctcgt
     2521 tgcccatcct ccatacatag tacgcacggc tgaggcggag cgttcggccc gtccgtgcgg
     2581 attgttttcg attagttgtt ggcctccaat ttctgcagct cgaaattgcc ggtgaattgg
     2641 tccttatggg ccaggacaaa tgtttgcacc gacgccgtcg gagctgtgat cagcttgcgg
     2701 ccgctgaagg tcgcatactg gatttcgccg gggtggtcgt tcaggtattt atcgagccat
     2761 tggaagtcga tggctgacag cttgagcttc ccttcgggcc gctgcaggcg atagatcgtg
     2821 tggatcggca cgaggtgaaa cttgtagaag ccgacggcat ccatctcgat gtcttcgggg
     2881 aagagatcga ggaacgattc gccgtcgagt ttggccagtc gcccaatgaa gcggccctgc
     2941 tggccctctt cgtcggtgta gatcagccgg tacgacttat cgtcgagctt cgagaattcc
     3001 cagcgggcct tggcgccggg ctgctgccag gtgccgacga gggccggatc gaagtcgagc
     3061 gtctcctccg tatagacggc attcagcgat ggaatacagc cggccaggag tgtgagaccg
     3121 gcgagaagca agcagtggat tttcgtctcg taagtctcgt aagcagtcat ggcttatcac
     3181 ctcggtgggc agaacggcat cgatcagaca tcaaccagca ggctttcgcg ccgggcgaac
     3241 ggacggtttt cggctacgac catccgatcc tcgacctgcc gcactcccgc gacgcgccgt
     3301 acgcacgaca gggccagttg ccgttcgtaa cgggatcgca cccggccggt gatcgtcacg
     3361 accccctgct cgacctcaac ctcgattcgt tttgcgacgg ccaattggag cgattgcaga
     3421 aacagacgga tccggtgcgt caggtcctcg tcggcccagc gggccggctc cagatgcgaa
     3481 gtattggaaa tggtgcgtgt catcgttcga cgcctttagg agagggtttg ggagcaaaag
     3541 cgtctccact ttaacctgag caagccttat gccgcagaac tcgcccaggc atcgtattac
     3601 agacgaaata ggcaaaaaac cccctgtttt tctgcaaaaa gcgccttaac atcacgcgac
     3661 gggaacggcc cgcactccgc gagtagtgat cttcgtagcg cgctgtttcg accggtcgca
     3721 gcacggaccg cgcgccccgc cggcgcgcat tggctagact tgagtctgtc tgtgccccgc
//
LOCUS       DASUGB010000522         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1308232, whole genome shotgun sequence.
ACCESSION   DASUGB010000522 DASUGB010000000
VERSION     DASUGB010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308232"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFV87_10020"
     CDS             complement(<1..200)
                     /locus_tag="VFV87_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104137.1"
                     /translation="MPQVRKAELVEGVVYMPSPVSTDGHAAPHSHLVTWLGTYHAFTP
                     GTQAGDNGTLHLDIDNEPQPDA"
     gene            complement(389..1030)
                     /gene="trmB"
                     /locus_tag="VFV87_10025"
     CDS             complement(389..1030)
                     /gene="trmB"
                     /locus_tag="VFV87_10025"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX5104138.1"
                     /translation="MGRRALRKIDPTLDLSRHYREAADVPATFDAAELFGRIAPLEVE
                     VGSGKGLFLAGASQTATDHNFLGIEVAQKYARYAAAKLARQGSANAVLVHGDALALFR
                     QWFAADTLAAVHVYFPDPWWKKRHKKRRVLNDDFLRDVQRTLLPGGRLHFWTDVEEYF
                     QTSLALIAATTNFSGPLPVAESPAEHDLDYRTHFERRTRKGSLPVYRAEFEKG"
     gene            complement(1124..1657)
                     /locus_tag="VFV87_10030"
     CDS             complement(1124..1657)
                     /locus_tag="VFV87_10030"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX5104139.1"
                     /translation="MAEKEFLIDLSQFDFDRPIATIEDIRQVNPQRFEMEQLTAIVHI
                     DQEVWSCVGYKDVTDHEFWVRGHMPGLPLMPGVVMLEAVAQLCSYVTQKHDLLGAAMV
                     GFGGLEEVRFRDPVLPGDRLIVMCRLDKVRRGRMIVCRFQGAVKDRVVIEGVLKGIPI
                     PVEALQALKAKNQEART"
     gene            2094..2438
                     /locus_tag="VFV87_10035"
     CDS             2094..2438
                     /locus_tag="VFV87_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5104140.1"
                     /translation="MAADHVLDGDELKQALADLPGWEVRDGWLRRTFNTPGWPHTMLL
                     AQAIGYLAEAAWHHPDLKLGYAACTVLLQTHRVKGITLSDIELAQKITEVALWKPAEG
                     AALEGFPKKWVH"
     gene            complement(2476..2901)
                     /locus_tag="VFV87_10040"
     CDS             complement(2476..2901)
                     /locus_tag="VFV87_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5104141.1"
                     /translation="MILLDTDHLVFFLRTSSSEANRLRDRLRHEPDDPRIAIVSAEEI
                     FRGWLSEIRRKSDVAKQISPYHEFQRAIATLANWQILPWDAQAVSQFKALKQTKIRVG
                     TMDLKIASIALAHGATLLTRNTADFERVPGLQIEDWLAS"
BASE COUNT          597 a          958 c          970 g          629 t
ORIGIN      
        1 aaagcatctg gctgtggctc gttgtcgatg tccaaatgga gggtgccatt gtcgcccgct
       61 tgcgtgccgg gtgtgaaggc gtgatacgtc ccgagccaag taacgagatg cgagtgcgga
      121 gctgcgtggc cgtccgttga cacgggtgat ggcatataga cgaccccttc aacgagctca
      181 gctttcctga cttgaggcat cgcatgatag cgtcgctcaa attcgttgcg agtcagacgg
      241 tcgccggcaa caagaggtgg aatctccgct gaagttgaca cttgtcgctc gggagtagca
      301 gcgatgctca ttgcggtgaa caacagaggg aatgcgaagt cttcggctga ccagccaaac
      361 tatattctag ctgactgcgg aaggacaatc agcctttttc gaactccgct cgatagaccg
      421 gcagcgagcc tttgcgcgtg cggcgctcga agtgcgtgcg gtagtcgaga tcgtgctcgg
      481 cgggcgattc ggcgacgggc agtgggccgg agaaattggt ggtggcggcg atgagggcga
      541 gcgacgtctg gaagtactct tccacgtcgg tccagaaatg cagccggccg ccggggagga
      601 gcgtccgctg gacgtcgcgg aggaagtcat cattgaggac gcgccgcttc ttgtgccgct
      661 tcttccacca gggatcgggg aaatagacgt gcacagccgc gagcgtgtcc gccgcaaacc
      721 actgacgaaa gagggccagt gcgtcgccgt ggacgaggac ggcgttggcg ctgccttgcc
      781 gcgcgagctt cgccgcggca tagcgggcgt acttctgcgc gacttcgatg ccgaggaagt
      841 tgtggtccgt cgctgtttgc gatgcgccgg ccagaaacag ccccttcccg ctgccgacct
      901 cgacttccag cggcgcgatt cgaccgaaca gttcggctgc gtcgaatgtc gccgggacat
      961 cagccgcttc gcgatagtga cggctcaggt cgagcgtggg atcgatcttg cgaagcgccc
     1021 ggcggcccat gggcaaatat ccaagaacca aagatccaag aaccaagcca gtcccgcgat
     1081 tcgtccttgg ttcttcggtt cttgtttctt ggttccgttc gtactaggtt cttgcttctt
     1141 ggttcttcgc cttgagcgcc tgcagcgctt cgaccggaat cggaatcccc ttcaggacgc
     1201 cctcgatcac cacgcggtcc ttgactgcac cttgaaagcg gcagacgatc atccgcccgc
     1261 ggcggacctt gtccagccgg cacatcacaa tcagccggtc gcccggcagc acgggatcgc
     1321 ggaaccgcac ctcttccagc ccgccgaagc ccaccatcgc ggcgccgagg aggtcgtgct
     1381 tttgcgtgac gtagctgcaa agctgtgcga ccgcttcgag catgacgacg ccgggcatca
     1441 gcggcagccc cggcatgtgg ccgcgaaccc agaattcgtg gtcggtcacg tccttatagc
     1501 cgacacacga ccagacctct tgatcgatgt ggacgatggc cgtgagttgc tccatctcga
     1561 agcgctgcgg attgacctgg cgaatgtctt cgatcgtcgc aatcggacgg tcgaagtcaa
     1621 actgcgacag gtcgatcaga aattctttct cggccacgaa tcgcttcctc gcctgaaaaa
     1681 gccgccggga cgccagccgc caggtgcgga cgtgcttctt gggtagccgc gttcgccaga
     1741 acgccggttt tcggcctgat cctgcacgct ggcgagtgcg gccaccactc acctcattcc
     1801 acggttctac ttaggtacgc acgtgctttc gcttggcctt gcgggccttg gcattggcgg
     1861 gccgtttgcc gtgattggct ttcgtgtgcg tacgatgggg cttggccatt tccgtatcct
     1921 tccaaaacgc gagtgcggga gtggtaattg gagaaacctt gagcgtagca aagtctgcgg
     1981 gcagtgagaa ggctgacctc gcgcttgaaa ccgcccgccg cattccgtcc gtcggccagg
     2041 cgcgcgacaa tggcccagcg cctcttgcgc ctcgaattct ggaggaccga accatggctg
     2101 ccgatcacgt cctggatggc gacgaattga aacaggccct ggccgatctg cccggctggg
     2161 aagtccgcga cggctggctg cggcggacat tcaacacccc cggctggccg cacacgatgc
     2221 tgctcgctca ggcgatcggc tacctggccg aagccgcctg gcaccatccc gatctcaagc
     2281 tgggctacgc ggcctgcacg gtgctcctcc agacgcaccg cgtgaaaggc atcaccttga
     2341 gcgatatcga gctcgcccag aagatcaccg aagtcgccct ctggaagccg gccgaagggg
     2401 cggcgctgga ggggtttcca aagaagtggg tgcattgagc atcgtgatga atccgccaac
     2461 caatgcacgg gaatctcacg acgccagcca atcctcgatc tgcaaacccg gcacgcgctc
     2521 gaaatccgcc gtgtttctcg tcagcagcgt cgcgccgtgc gcgagtgcaa tgctggcgat
     2581 tttgaggtcc atcgtgccca cccggatttt cgtctgtttg agcgccttga actggctcac
     2641 ggcctgcgcg tcccacggca ggatttgcca gtttgcaagc gttgctatgg cccgctgaaa
     2701 ttcgtggtag ggtgaaatct gctttgccac gtccgatttg cggcgaattt cggacagcca
     2761 gccgcgaaag atctcttcgg cacttacgat ggcaatgcgc ggatcatctg gctcgtgacg
     2821 gagacggtcg cggagacggt ttgcctcgga agaggaggta cgcagaaaga aaacgagatg
     2881 gtccgtgtcg agaaggatca ctccaagtcc tcaagacttt cgcgattgac ttgatcgcgg
     2941 tactctcgtc cgaaccggac cgcctcgtca aactccgggc aattcgcaaa tgctccgaac
     3001 gtcgcatccc atgccccccg cggaggacga gtggcgacga gttgcctaag ttcggccaat
     3061 tcccgctcga ggacggctac tcgctgttcc aattcaccga cggtcatttc gcttctccaa
     3121 cggagtcgtg cagcatcaga atcatagcac tacc
//
LOCUS       DASUGB010000523         6612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2004285, whole genome shotgun sequence.
ACCESSION   DASUGB010000523 DASUGB010000000
VERSION     DASUGB010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004285"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFV87_10045"
     CDS             complement(<1..806)
                     /locus_tag="VFV87_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104142.1"
                     /translation="MLSPQEFARLLQDKLIEAGENRPLEFRADLFGFIPRGGDLGSIF
                     PLDNYYRQYLAAASDGERQRVARQFTSDWLRHAKAGAPVAPGAGGAPAAFAPSPLASG
                     HGAPVVVPNPLAGGPLPRPSGTPPRPSVGCGVRAVMAIIGVVGLAFVCFCGAPLAMLG
                     MAKNRPGNFGPGNGPRFGPDWEFHAPADVQVTEFVGGRGGGPRFYVDPQRRPVIGLAY
                     STDNWGGQQVIGSLAPVYDAHDVPENDFRRETHVVRAKEGYVVGGLEVDAG"
     gene            complement(884..2011)
                     /gene="rsgA"
                     /locus_tag="VFV87_10050"
     CDS             complement(884..2011)
                     /gene="rsgA"
                     /locus_tag="VFV87_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5104143.1"
                     /translation="MASKKKIRTEFRKEHQGRRRQKDLTRDLARDERAEDRLVKSERL
                     TGKGELTRKRTIVGQEADAEAAGFAVQREADPTALRGRVLAVHGLRCVVQADDGREYR
                     CATRRVLKNLSTDLRHVVVAGDVVFLRPEQYGDSLIERIEPRDHILSRTSRGKQHILV
                     ANVDRLVIVSSAAEPHLKPNLIDRFLVEAERMRIEPTVCINKIDLVDPADLQPLAGVY
                     GQMGYRVILASATTGIGIASLRRLLQGKESVVAGQSGVGKSSLLNAVEPELALRIGEV
                     SRENEKGKHTTTVARLIPLRIGGYVVDTPGIRQFQLWDIVPEEVAGYYRDIRPYVSLC
                     RFPDCTHTHEANCAVKDAVADGQLDARRYESYCHLREGDES"
     gene            complement(2022..2720)
                     /locus_tag="VFV87_10055"
     CDS             complement(2022..2720)
                     /locus_tag="VFV87_10055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5104144.1"
                     /translation="MTNHSDQHKIWQHFQNASPESFAAAHPRLEFLIGQIAQRTSGRQ
                     PAVLNIGIGDGFFERRAKARGWKVHSLDPDEQAVERLKGEGISAQVGTIEHILHESES
                     LDFIVASEVLEHLTEDQRAAGLAEIARVLRPGGWFLGSVPHAENLAEQHAVCPHCGQV
                     FHRWRHQASFTLASVRQMLAGPFVVEKLKRTAFVDLWSRGPLGFVKGSLRVLLAKLGQ
                     PIAVPTIWWVARKE"
     gene            3026..3589
                     /locus_tag="VFV87_10060"
     CDS             3026..3589
                     /locus_tag="VFV87_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104145.1"
                     /translation="MPTQQPESEVDQLLLNARLRDELEPFLDESVHILDCRRLPTPAE
                     NEFLSQMLAWEKAPVLPIGKWFEPELTLPTPDQLTAEQLHELLWQTIEKLYSQRIVLD
                     FTEHLSDRQLYCLILRDILPSPEKKLDLPRNYLHWHFLDIDVQPEIWLTYYASDEERR
                     MFAEETGQKLPRKAAPPYPRRMPRRPG"
     gene            3766..4734
                     /locus_tag="VFV87_10065"
     CDS             3766..4734
                     /locus_tag="VFV87_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104146.1"
                     /translation="MPIRFACPHCHQKLSVSSRKAGTSADCPRCKQKLSIPTPPPEPP
                     KPAATSTPAPPPAPAAKPAAQPFKFPEPEASPASAPEAAVAVAEPPPEAEDAVWTPDP
                     VGFEGMELVYDTTDSTSAAAAPLIGSEHTELVAIPRYVLYLQGGLIGVVALVAFAIGL
                     LAGGAIFTQPPAPAEPEPVVLRGSVTYAAGPRNLPDEGAVIAIIPQSQSKPDDKAPVA
                     GLRPDDPTPGESHRGIAVLRGLGGGYARADANGRFEVRLPDRGRYMVLVVSSGAQVRS
                     IEDIQTGDILKLGPYFDNAADLLGDHRYKLTLETLRGDRDFNVAFE"
     gene            complement(4798..6117)
                     /gene="purD"
                     /locus_tag="VFV87_10070"
     CDS             complement(4798..6117)
                     /gene="purD"
                     /locus_tag="VFV87_10070"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5104147.1"
                     /translation="MKVLVVGNGGREHALAWKIAQSPRASRVFIAPGNAGTANEGENV
                     EIAAGDIPGLLKFVQQNSIDLTVVGPEAPLVAGIVDIFQREKLRVFGPSKAAAQLEGS
                     KVFCKNLLHGADVPTAEYRVFREGESATRYVMDRYASDEESCPLVVKANGLASGKGVF
                     VCSTRRDAVEAIDRIARQREFGSAGKQILIEERLDGQEASVLALTDGRTILTLPPAQD
                     HKRAHDGDTGPNTGGMGAYCPAPLVDERLLRNIEEHILVPTVHAMKRSRQPFKGVLYA
                     GLMMTPAQGPKVLEYNVRFGDPECQPLLLRLKSDLVDLIEATIDGKLDEIKPPEWDER
                     PAVCVVMASEGYPGDYEKGRPIRGLEEAGAIPDVKVFHAGTKRVDGQVVTAGGRVLGV
                     TALGNTIATAKLQAYTAVKCIRWQGAWCRKDIADKAMRAGGDAPRRK"
     gene            6327..>6612
                     /locus_tag="VFV87_10075"
     CDS             6327..>6612
                     /locus_tag="VFV87_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104148.1"
                     /translation="MLRLTLLSALSLSLLPIFPAASAQEGVDESPLPVQLVRAFPKLK
                     PRRPVAITHAGDGSNRIFILSAYGQIWVGENSQSLAELKTFLDIEYKVDYE"
BASE COUNT         1081 a         2282 c         2071 g         1178 t
ORIGIN      
        1 ccggcatcga cttccagtcc gccgacaacg tagccctcct tcgcgcgaac gacgtgcgtc
       61 tcgcggcgga agtcgttttc cggcacgtcg tgcgcgtcgt acacgggtgc cagtgagcca
      121 atcacttgct gcccgcccca attgtcggtc gagtacgcca ggccaataac ggggcggcgc
      181 tggggatcga catagaaccg cggccctccg ccgcggccgc cgacgaactc ggtcacctgg
      241 acgtcagccg gcgcgtggaa ttcccaatcg gggccgaagc gtggtccatt gccggggccg
      301 aaattccctg ggcgattctt cgccatgccg agcatcgcca gcggcgcgcc gcagaagcag
      361 acgaaggcca gtcccacgac gccgatgatc gccatgactg ctctgacgcc gcagccgacg
      421 ctcggccgcg gcggcgtgcc gctgggccta ggaagcgggc cgccggccag cggattgggg
      481 accacaaccg gcgcaccgtg gcccgatgcc agcgggctgg gtgcgaacgc cgccggcgca
      541 ccgccagcac cgggagcgac cggcgccccg gctttggcgt gccgcagcca gtcactggtg
      601 aactgcctgg cgacccgctg ccgctcgccg tcgctcgccg cggccagata ctggcggtag
      661 tagttgtcga gcgggaagat cgagcccaga tcgccgccgc gcggaataaa gccgaacaaa
      721 tcggcgcgaa attccagcgg ccggttctcg ccggcttcga tcagcttgtc ttgcagcaag
      781 cgggcgaatt cttgggggct gagcatgggt agggtgggct gtgcccaccg gctgggtttg
      841 gtgggcacag cccaccagca aggttcggtg ggcatagccc accctacgat tcgtcgcctt
      901 cgcgtaagtg gcagtagctc tcgtaccgcc gggcgtcgag ctggccgtcg gcgacggcgt
      961 ctttcacggc gcagttagcc tcgtgcgtgt gcgtgcagtc ggggaagcgg cagaggctga
     1021 cgtacggccg gatatcgcga tagtagcccg ccacctcttc cggcacgata tcccacagct
     1081 gaaattgccg gattcccggc gtatcgacga cgtagcctcc tatccgcagc ggaatcagcc
     1141 gggccacggt cgtcgtgtgc ttgcctttct cgttctcgcg gctgacttcg ccgatccgca
     1201 aggccagctc gggctcgacg gcattgagca gcgacgactt ccccacgccg ctttggccgg
     1261 cgacgacgct ctccttgcct tggagcagcc gccgcagcga tgcgatgccg atgcccgtcg
     1321 tcgccgaggc caggatcacg cgatagccca tctggccata cacgcccgcc aacggctgca
     1381 ggtcggccgg atcgacgagg tcgatcttgt tgatgcacac ggtcggctcg atccgcattc
     1441 gctcggcctc gacgaggaag cggtcgatca ggtttggctt gaggtgcggt tcggcggcgc
     1501 tggagacgat caccagccga tcgacgttgg ccacgaggat gtgctgcttg ccgcggctgg
     1561 tgcggctgag gatatggtcg cgcggctcga tgcgttcgat gagcgaatcg ccgtactgct
     1621 cgggccggag gaagaccacg tcgccggcga cgaccacgtg ccgcaggtcg gtgctgaggt
     1681 ttttcaggac gcgccgcgtg gcgcagcgat attcccggcc atcgtccgcc tggacgacgc
     1741 accgcaagcc gtggacggcc agcacgcgcc cgcgcaatgc cgtggggtcc gcctcgcgct
     1801 ggacggcgaa gcccgcagcc tcggcgtcag cctcctggcc gacgatcgtc cgcttacggg
     1861 tcagctcgcc tttgcccgtc agccgctcgc ttttgaccag ccgatcttcc gcccgctcgt
     1921 cgcgagccag gtcgcgcgtc aggtccttct gccgccgccg gccctgatgt tccttgcgga
     1981 actcggtgcg gattttcttt ttgctggcca tttccttcat tctactcttt ccgcgcgacc
     2041 caccagatcg tcggcacagc gatcggctga ccgagctttg ccaggagcac ccgcagcgag
     2101 ccttttacga agccgagcgg tccgcggctc cacaaatcga cgaaggcggt acgtttcagc
     2161 ttctcaacca caaacggccc ggcgagcatc tgccgcacgc tggccagcgt gaacgaagcc
     2221 tgatgccgcc agcggtggaa gacttgcccg cagtggggac agacagcgtg ctgctcggcc
     2281 aggttctcgg catgcggcac gctgcccaga aaccagccgc cgggacgcaa aacgcgggcg
     2341 atctcggcca atcctgccgc gcgttgatcc tcggtcagat gctccagcac ttccgacgcc
     2401 acgataaaat cgagcgactc gctttcgtgc agaatgtgct caatcgtccc gacctgcgcg
     2461 gaaatgcctt cgcccttcag ccgctctacc gcctgctcgt ccggatcgag cgaatgcact
     2521 ttccagccgc gggctttcgc gcgccgctcg aagaatccgt cgccaatgcc gatattcagc
     2581 accgccggct gccggccgct ggttcgctgg gcgatctgtc cgatcaaaaa ctccagccgc
     2641 ggatgagcag ccgcgaacga ctcgggcgag gcgttctgga agtgctgcca gatcttgtgt
     2701 tggtcggaat ggttggtcat cagcactcaa tgatacctgc gggcgcgttc atcgctgcta
     2761 tcggaagcgt gaagcatgtt tcaaacccga agcgcgtgaa attgcccagt ttgcgcctgc
     2821 ggaaaggtcg cttgctggac ctcgcgggtc gtgcggcttg tacgattcgc aagctggaaa
     2881 acgcgggaaa tcgcggcctt tggcggtggt ttttctcttg tggcgggccg cggcctggat
     2941 aaagtcgaat cctgacgacc ttgttgcggc actccttcca ggtgttgccg ctcgtgtgct
     3001 gaacgatggt cccagggccc ctcccatgcc tacgcagcaa cctgaatcgg aagtcgatca
     3061 gctcttgctc aacgcgcgcc tgcgcgacga attggagccg ttcctggacg agtcggtcca
     3121 cattctcgat tgccgccgcc tgccgacgcc cgccgagaac gaattcctct cgcaaatgct
     3181 ggcctgggag aaggcccccg tgctgccaat cggcaagtgg ttcgagccgg agctgacgct
     3241 gccgacgccg gaccagctca ccgccgaaca actgcacgag ctgctgtggc agacgatcga
     3301 gaagctgtat tcgcagcgga tcgtgctcga ttttaccgag cacctgagcg accggcagct
     3361 ctattgtttg atcctgcggg acatcttgcc ctcgccggag aagaagctcg acctgccgcg
     3421 gaactatctg cactggcact tcctcgatat cgacgtgcag ccggaaatct ggctcacgta
     3481 ctacgcctcg gacgaagaac gacggatgtt tgccgaggag accggccaga agctgccgcg
     3541 caaggccgcg cctccctatc cgcggcggat gccacggcgg ccggggtagg caacgcgtcc
     3601 acgtggcatt tcaccggcct cgtttaacgc gcagccgaag gcgtagccga tagcaggttc
     3661 cacccggaaa cggctacgcc tccgcttcgc ttcggctacg cgttaaacga gcgattgcat
     3721 agccgcaaac tgggcggcgt ccaccggcga tgctactcta aatgcatgcc catccgcttt
     3781 gcctgcccgc attgccatca gaagctgagc gtcagttcgc gcaaggcagg cacctcggca
     3841 gattgcccgc ggtgcaagca gaagctctcg attcccacgc ctccgcccga gccgcccaag
     3901 ccggctgcta cttctacgcc agcgcctccg ccggcgcctg cggcgaaacc cgctgcgcag
     3961 cccttcaaat tcccagagcc ggaggccagc cccgcctctg cgcccgaagc cgcggtcgcc
     4021 gtcgccgagc cgccgccgga ggcggaggac gcggtctgga cgcccgatcc ggtcggcttc
     4081 gaggggatgg agttggtcta tgacaccacg gactcgacct cggctgccgc cgcaccgttg
     4141 atcggcagcg agcacaccga gctggtcgcc attccgcggt acgtgctcta tctgcagggc
     4201 ggcctgatcg gcgtcgtcgc cctggtcgct ttcgcgatcg gactgctcgc cggcggggca
     4261 atcttcaccc agccccccgc gcccgcggag ccggagcccg tcgtgctccg cggcagcgtg
     4321 acctatgcgg ctggcccgcg gaacctgccc gacgaagggg ccgtcatcgc gatcatcccg
     4381 caatcgcaat cgaagccaga cgacaaagcg ccggtcgcgg gcctgcggcc ggacgatccg
     4441 acgccgggcg aatcgcaccg cggcatcgcc gtgctccgcg gcctcggcgg cggctacgcc
     4501 cgggcggacg ccaacggccg cttcgaggtc cgcctgcccg accgcggccg gtacatggtc
     4561 ctcgtcgtct ccagcggagc gcaggtccgc tcgatcgaag acatccagac cggcgacatc
     4621 ctcaagctcg gcccctactt cgacaacgcc gccgacctgc tcggcgacca tcgctacaaa
     4681 ctcacgctgg aaacgctccg cggcgatcgc gatttcaatg tggcgtttga gtaagtgggg
     4741 cagacattct tgtctgccac gtccccattt atggcagaca agaatgtctg ccccacgtta
     4801 cttgcgccgt ggagcgtccc cccccgctct catcgccttg tccgcgatgt ccttgcggca
     4861 ccaggcgcct tgccagcgga tgcatttgac ggcggtgtag gcctgcagct tggcggtggc
     4921 gatggtgttg cccagggccg tcacgccgag gacgcggccg ccggcggtta ccacctggcc
     4981 atcgacccgc ttcgtcccgg cgtggaagac cttcacgtcc gggatcgccc cggcttcctc
     5041 cagcccgcgg attggccggc ccttttcgta atcgcccgga tagccctcgc tggccatcac
     5101 cacgcacacc gcgggccgct cgtcccactc gggcggcttg atctcgtcca gcttgccgtc
     5161 gatcgtcgcc tcgatcagat cgacgaggtc cgacttcagc cggagcaaca gcggctggca
     5221 ctccggatcg ccgaaacgga cgttgtattc cagcaccttc ggcccctggg cgggggtcat
     5281 catcaggccg gcatagagca cgcccttgaa cggctggcgg gagcgcttca tggcgtgaac
     5341 ggtcggcacg aggatgtgct cctcgatatt gcggagcagc cgctcatcga cgagcggggc
     5401 ggggcaatag gcccccatgc cgccggtgtt cgggccggtg tcgccgtcgt gggcccgctt
     5461 gtggtcctgg gctgggggca gggtgaggat cgtccggcca tcggtgagcg ccaggacgct
     5521 cgcttcctga ccgtccagcc gctcctcgat cagaatctgc tttcccgccg agccaaactc
     5581 ccgctgtcgg gcaatccggt cgatcgcctc gacggcatca cggcgcgtgc tgcagacaaa
     5641 gacacccttc cccgaggcca ggccattggc cttcacgacc agcgggcacg attcctcgtc
     5701 gctggcataa cggtccatca cgtaccgggt cgcactttcc ccttcgcgga acacgcgata
     5761 ctcggccgtg ggcacatccg ccccgtggag caagttcttg caaaagacct tgctcccttc
     5821 gagctgcgcc gctgccttcg aggggccaaa gacccttagc ttctcgcgct ggaagatatc
     5881 tacgattccg gccaccagcg gcgcttccgg gccgacgacc gtcaggtcga tcgagttctg
     5941 ctggacgaac ttcagcaagc cggggatatc gcccgcggcg atctcgacgt tctccccttc
     6001 attcgcggtg ccggcgttgc cgggcgcaat gaagacgcgg ctggcgcgcg ggctctgggc
     6061 gatcttccag gccagcgcat gctcccgccc gccattgcca acgaccaaaa ccttcatgga
     6121 gagaatccct gcaccagagt gcggtccgac cactcgaata tagaaaaccg gccgctcccg
     6181 cgtaagttgg ccaccctgcc cgtccgagta tgcccaattg tagcggctgg agcgggtcgc
     6241 ggttatgata gccgagcttc cgcactggcc cggctgatcg gctgccgagc attcaagatc
     6301 aacgccaacc cctgtacccc tcccccatgc tgcgactcac cttgctttcc gcgctttcgc
     6361 tgtctctctt gccaattttc cctgccgcgt ctgctcagga aggcgtcgat gagtcgccct
     6421 tgccggtgca gctcgtccgc gcgtttccga agctcaagcc gcggcggccc gtcgcgatta
     6481 cgcatgccgg cgacggctcg aaccggattt tcatcctttc ggcgtacggg cagatctggg
     6541 ttggcgagaa cagccagagc ctcgcggagc tgaagacgtt cctcgacatc gaatacaagg
     6601 tcgattacga gg
//
LOCUS       DASUGB010000524         4335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_613746, whole genome shotgun sequence.
ACCESSION   DASUGB010000524 DASUGB010000000
VERSION     DASUGB010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613746"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2823
                     /locus_tag="VFV87_10080"
     CDS             <1..2823
                     /locus_tag="VFV87_10080"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX5104149.1"
                     /translation="SLEVPLERVRHPLEYRLAAGSTTTKEYKVQVIYPLKIARIQAAI
                     EPPAYTGQSVSTTEGGNITGLAGSKVKLAIELDRPAESAWLVLHDLVTRRQALQAEPQ
                     VQKLPLTIEGSRLLADLELNADQTYEVIAHSSDDMELPENKYRIRVRQDEPPQVWFES
                     PAEALEVHTLAEILMRLRVSDDYGLTRAGVMFEVNNEEEYPLLAQDFQVAADAAEELK
                     STGKLAPQTRATLEKVLPLEHFTLTQQDSVMYFAFAEDNRPGSNQRTESDLRFIDIRP
                     FRRLYKALDADGENPMGQQGPQLKSLEELIARQRYALNRTIQLTRRFEQSGQADLAGV
                     DALIKFEAELAKSTRELAEGLEARGIDETELLYQAETAMFGATDSLSAGKYDTATLQQ
                     RDALKYLIEGRNRLEVFIRKNPNRAQLAALRQFDRMQRQKLRRPKTDEEEAREVAERL
                     EDLADKQDFVYATLAGIKMEGMKTAGNSGGSSGQSTMPPKMPPMPNPDGSGESTTPMP
                     MPKEGASPEATKSEDDQPMEGSAGQEPAEGTKPGGPSREELEDQQLDIALEAREIEKI
                     LGRLPRATDLAKERIAAAAQLAEEGAQALGSGKMDEARDTTGSARDGFAELAEQVRGL
                     LAEEQADRVAAAQRLAAELARQQQEFVDRLAQSENKGGIGNMPPEEKSQASGANPMNQ
                     NQNDSDMPGLGAAAEKIAERAQTLADVLGGAAKATTPEEEAAAKKVEELVSGMGIGEL
                     TERLQNLPGQVGEGKKEEAKANAGDGSERLESTAEQLANLHRSIVSPQIDELAKVEQQ
                     LTALDEEIDVLETPTQITGWHMDATELMDELDKAGVPEELRQEFMDELKNGGWGPDAA
                     RRGWNWGRVEGRYYIAPDGYRVRLSRLLASVRGRMQELLLGDLAASGDEPIPPQYQEF
                     VDRYQQVLTSEGRPAPRTPAEPAPAP"
     gene            2834..3670
                     /locus_tag="VFV87_10085"
     CDS             2834..3670
                     /locus_tag="VFV87_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104150.1"
                     /translation="MHSLVIAASLLAQPPAAGSAEGAPADRHDPVRAEYLADAQRCLF
                     HHDAQKQHELELIEKPVMRWATDDDWSGDVFVWTRESLPEVVGCILSGPGNAGRPVYH
                     EFHVLGEQPIAPITLQTRRLWQPSEGLKRLPVPEAPTPAETAAGRLVQMRQMARDFTA
                     HMEADGTWELRLLPQPVFRYRSESAGVTDGALFTYVWSKGTDPELVLLLECRMADKGP
                     NWHFAPLRLSTRELWLQHHESEVWHVAAHQEPQTETTMIYTTAYARTMATPPTAGSEQ
                     EN"
     gene            3698..>4335
                     /locus_tag="VFV87_10090"
     CDS             3698..>4335
                     /locus_tag="VFV87_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104151.1"
                     /translation="MLLAQSTTQTFLPPFSALTILCLIAGCAVGALLLGWLLGPANPV
                     ARRWSLWAIRGAILAIIVIVILNPVQVDQLPGPQQRPEMFYLVDTSASMQMGNPRSRW
                     DQTVGFIREAQQKAPRSPAVVKPFRFGQRLAAIDRPAQIGLGPWDLPTITPVSAKAGE
                     RPSRPIAPTDGDTRLQAALRQISSRFSRIPPQGIVLFSDGRVHDDTGLEQIAA"
BASE COUNT          859 a         1367 c         1447 g          662 t
ORIGIN      
        1 tcgctcgaag tgcccctgga gcgcgtgcgc catccgctgg agtatcgcct ggcagcggga
       61 tcgacgacga cgaaggagta caaggtccaa gtcatttatc cattaaagat tgctcggatc
      121 caggcggcga tcgagccgcc cgcttatacc ggtcaatccg tctcgacgac cgagggcggc
      181 aacatcacgg gcctcgccgg ctcgaaggtg aagctggcca tcgagctgga tcgcccggca
      241 gagtcggcgt ggctcgtgct ccacgacctc gtcacgcgcc gccaagcgct acaggccgag
      301 ccacaggtcc aaaagctgcc gctgacgatt gaaggctcga ggctgctggc cgatttggag
      361 ctgaacgccg accagacata cgaggtaatc gctcattcct ccgacgacat ggagttgccc
      421 gagaataaat accgcattcg cgttcggcag gacgagccgc cgcaagtctg gttcgagagc
      481 cccgccgaag cgctcgaagt ccatacgctg gcggaaatcc taatgcgttt acgcgtcagc
      541 gacgattacg gcctgacgcg ggccggcgtc atgttcgaag tgaataatga agaggagtat
      601 ccgctgctgg cgcaggattt ccaggtcgcg gcggatgcag cggaggaatt gaagtcgacg
      661 ggcaagctcg cgccgcagac gcgggccacg ctggaaaaag tcctgccgct ggagcacttc
      721 acgcttacgc agcaggatag cgtcatgtat ttcgcgtttg ccgaggacaa tcgccccggc
      781 tctaatcagc ggaccgagag cgatctacgg tttatcgata ttcggccgtt ccggcggctg
      841 tataaggcgc tggatgccga cggtgagaat ccgatgggcc agcaaggacc gcagctcaag
      901 tcgctggagg agctgatcgc gcgccagcgg tacgcgctca accgcacgat tcaacttacc
      961 cgccgcttcg agcagagcgg tcaggcggac ctggccggcg tcgatgcgct tatcaagttt
     1021 gaggcggaac ttgccaaatc gacgcgcgag ctggccgaag ggctcgaagc ccgcggcatc
     1081 gacgagaccg agctgctcta ccaggcggag acggcgatgt tcggcgcgac cgattcgctt
     1141 tccgctggca agtacgatac tgcgacgctc cagcagcgcg acgcccttaa atacctgatc
     1201 gaaggccgca accggctgga ggttttcatc cgcaagaatc cgaaccgggc gcagctcgcg
     1261 gcactgcgac agttcgaccg catgcagcgg cagaagctcc gccgacccaa gaccgacgag
     1321 gaagaagccc gcgaagtcgc cgaacggttg gaggatctgg ccgataaaca ggacttcgtc
     1381 tatgccacgc tggcgggaat caagatggaa gggatgaaga ccgctggcaa ttcgggcggc
     1441 agcagcgggc aatcgacgat gccccccaag atgccgccga tgccgaatcc cgacggcagc
     1501 ggcgaatcga caacgccgat gcccatgccg aaggaaggtg cttcccccga ggcaaccaag
     1561 tcggaagacg accagccgat ggaaggcagc gccggccaag agcccgccga agggacgaag
     1621 cctggcgggc cgtcgcgcga ggagctggaa gatcagcagc ttgacatcgc cctggaggcc
     1681 cgcgaaatcg agaagattct cggcaggttg ccgcgggcga ccgacctggc caaggagcgc
     1741 atcgcggccg cggcgcagct cgccgaggaa ggggcgcaag cccttggcag cggcaaaatg
     1801 gacgaagccc gcgatacgac cggatcagcc cgcgacgggt tcgcggagct ggccgagcag
     1861 gtccgcggcc tgttggccga ggaacaagcc gaccgcgtcg ccgcggcgca gcgtctggcg
     1921 gccgaactcg cccgccagca gcaggagttt gtcgatcgcc tggcccaatc ggaaaacaag
     1981 ggcggcatcg gcaatatgcc gcctgaggag aagtcgcaag cgtccggcgc caatccgatg
     2041 aatcagaatc agaatgacag tgacatgccc ggcctgggcg ctgcggctga aaagattgcc
     2101 gagcgcgccc agacgctggc cgacgttctc ggcggggcgg cgaaggccac gacgcccgag
     2161 gaagaagccg ccgccaagaa ggtcgaagag ctggtatccg gcatgggcat cggcgagctg
     2221 acggagcgcc tgcagaatct gcccggccag gtaggtgaag gcaagaagga agaggccaag
     2281 gccaatgccg gcgacgggag cgagcgcctg gaatcgacgg ccgagcagct tgccaacctt
     2341 caccgctcga tcgtttcgcc gcagatcgat gagctggcga aggtcgagca gcagctcacg
     2401 gcgctcgacg aagagatcga cgtgctcgaa acgccgacgc agatcaccgg ctggcacatg
     2461 gatgccacgg aactgatgga cgagctggac aaggccggcg tgcccgagga gttgcggcag
     2521 gaattcatgg atgagctgaa aaacggcggc tgggggccgg atgcggctcg ccgcggctgg
     2581 aactggggcc gcgtggaagg ccgctactac atcgcacccg acggataccg cgtgcggctg
     2641 tcgcggcttc tggcgtctgt ccgcgggcgg atgcaggaat tgttgctggg cgatctggcc
     2701 gccagcggcg atgagccgat cccgccgcag taccaggaat tcgtcgatcg ctaccaacag
     2761 gtgctgacca gcgaaggccg gccagcgccg cggacgcccg cggagcccgc tcccgcgccc
     2821 tagaatgacc gttatgcaca gccttgtcat cgctgcgagc cttctcgccc agccgcctgc
     2881 ggctgggtcc gcggaaggtg cgcccgccga tcgacacgat cccgttcggg ccgagtatct
     2941 ggccgacgcg cagcgatgcc tgttccatca cgatgcccag aagcagcacg agttggagct
     3001 gatcgagaag cctgtgatgc gctgggccac ggatgacgac tggtcgggcg acgtctttgt
     3061 ctggacccgc gagagtctgc cggaagtcgt tggctgcatc ctttcgggac cgggaaatgc
     3121 cggccgtcct gtctatcacg agtttcacgt tctcggcgag cagccgatcg cgccaataac
     3181 tctgcaaacc cgccgcctct ggcagccaag cgaaggactg aagcgcctgc cggtgcccga
     3241 agcgcccacg ccggctgaaa ccgctgccgg gcggctcgtg caaatgcgcc agatggcgcg
     3301 agacttcacg gcccacatgg aagcggacgg cacgtgggaa ttgcggctcc tcccgcagcc
     3361 ggtgtttcgc tatcgcagcg agtcggccgg cgtgacggac ggcgcgctct tcacgtacgt
     3421 gtggtcgaag ggaaccgatc cggagttggt gctgctcctg gaatgtcgga tggcggacaa
     3481 aggccccaac tggcacttcg ccccgctgcg gttatccacg cgcgagctgt ggctccagca
     3541 tcacgaaagt gaagtctggc acgtggctgc gcaccaggag ccgcagacgg aaacgacgat
     3601 gatctatacg acggcatacg ccagaacgat ggcgactccg ccgacggctg gctcggaaca
     3661 agagaactga caaccgctct cgcgagcgac caacgatatg ctcctggcac aatccacaac
     3721 gcagaccttt ctgccgccct tctcggcgct gacgatcttg tgcctgattg caggctgcgc
     3781 cgtgggcgcg ctgttgctgg gctggctgct gggaccggcc aaccccgtgg cgcggcggtg
     3841 gagtttgtgg gcgatccgcg gggcgattct ggcgatcatc gtcatcgtga tcctgaaccc
     3901 cgtgcaggtc gatcaactcc ctggcccgca gcagcggccc gagatgttct acctcgtcga
     3961 tacgtcggcc agcatgcaga tgggcaaccc gcgctcgcgc tgggaccaga cggtcggctt
     4021 catccgcgaa gcgcagcaga aggcgccgag gtcgcccgcg gtcgtcaagc cgtttcgctt
     4081 cgggcagcga ttggcggcga tcgaccggcc ggcgcaaatt ggcctgggcc cttgggacct
     4141 tcccacgatt acgcccgttt ccgccaaagc tggcgagcgc cccagccgtc ccatcgcgcc
     4201 gaccgacggc gatacccgcc tgcaggccgc tttgcgtcag atttccagcc gctttagccg
     4261 tattccgccg cagggcatcg tcctgttttc cgatggccgg gtgcacgacg acacggggct
     4321 ggagcaaatc gctgc
//
LOCUS       DASUGB010000525         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1032327, whole genome shotgun sequence.
ACCESSION   DASUGB010000525 DASUGB010000000
VERSION     DASUGB010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032327"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFV87_10095"
     CDS             complement(<1..193)
                     /locus_tag="VFV87_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104152.1"
                     /translation="MRRWSLLVAGCWLWQIAACASGQDFATELFDVRQRLEALERENQ
                     ELRREVLERLPPVDAEGEAF"
     gene            complement(400..795)
                     /locus_tag="VFV87_10100"
     CDS             complement(400..795)
                     /locus_tag="VFV87_10100"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX5104153.1"
                     /translation="MPDHIPKSKLPFDSLAEQRIRAAQAAGEFDNLPGFGQPIPGIDE
                     PHDELWWVKDKLKREQLSALPPALAIRLDVEQTLAQLPSLATEPAVRDEIAALNERIR
                     QASFGVIWGPSVDVTPLDADEIAARWRSQ"
     gene            976..2487
                     /gene="proS"
                     /locus_tag="VFV87_10105"
     CDS             976..2487
                     /gene="proS"
                     /locus_tag="VFV87_10105"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5104154.1"
                     /translation="MAKTAITPTRQDDYPEWFQQVIRAADLAEISAVRGCMVIKPWGW
                     SIWENMQRVLDGMFKATGHENAYFPLFIPLSFLQKEADHVEGFAKECAVVTHHRLVPN
                     SEGKLIPDPASQLEEPLIVRPTSETIIGAAYAKWVESYRDLPILINQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATAEEAQEETMRMLGVYADFAENHMAMPVIQGEKTAGERF
                     PGAVATYAIEAMMQDRKALQSGTSHFLGQNFSKAQEIKFQDQNGQQQFAWTTSWGVST
                     RLIGGLIMTHSDDDGLVLPPRLAPKHIVLLPIYRSDDEKSQVLPYVESLKKELEAQDY
                     ADGKVRVMIDDRDIRGGEKNWHHIKRGVPLRAEVGPKDIAKNGVFLARRDTSEKAGVE
                     RTQLVATIGQRLTEIQDNLFQRALKFRQENTRTIDKLDDFLAYFTPKNADNPEIHGGF
                     ALCHYCEGPEMDEFLKKHKVTIRCIPLGGAGEGGTCFLTGKPSAKRAVFAKAY"
     gene            2542..2829
                     /locus_tag="VFV87_10110"
     CDS             2542..2829
                     /locus_tag="VFV87_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104155.1"
                     /translation="MYDGDLEEHSQQGIRWNQSVALFLGLVAVFGLGYAAVALWQEAR
                     LPNGRELWHGLAVSSVLVAAGAVAMWRRSLVVGFVLVVFAMVFFGVWMPDR"
     gene            2905..3387
                     /locus_tag="VFV87_10115"
     CDS             2905..3387
                     /locus_tag="VFV87_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104156.1"
                     /translation="MPCYHFIFHGHGTWMPDVDDGYVTRDDGHLPQDTAMAARYRDLM
                     VEEEVTFTEDQEQTMIDEVHVAAGKQKFRVHYIATEPTHIHILVSWKDDRPFEKLRAS
                     IRQSISRRLGKDYERRTWLSEGGSRRKVSSQEHFDYLVAIYLPKHGGWKWSEERGLFR
                     "
     gene            3459..4268
                     /locus_tag="VFV87_10120"
     CDS             3459..4268
                     /locus_tag="VFV87_10120"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX5104157.1"
                     /translation="MALILQTNQHYLRNAQTLGHLAIAAYAPKPAEDAAFKKTMFTEA
                     TSFVHGPTSSLGYWTVSDDHVVLAFAGTNDIRDWLTNIHFAMVPELGGKAHRGFSQAL
                     DAIWSIFFTKIKLHTDAGRKLWITGHSLGGALATLAARRLPTSMKPAGVYTFGQPRVG
                     DTTYAKKYKLKHHRFVHNKDIVPTVPSRFIPGAFPPAFYTHVGSLEFFDKNKKLVGSS
                     SDELGLMPELQEALSQLSTTTTEAQAKELILDGIRDHSMKAYLECLAKNVP"
     gene            4426..4953
                     /locus_tag="VFV87_10125"
     CDS             4426..4953
                     /locus_tag="VFV87_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HEX5104158.1"
                     /translation="MAHTQPHIAVLRILAAFSRYPKGLDWARRTAEAAWGPVALESER
                     FEHRETNYYDATMGTDLEKCFFAFETLADPAALAEWKLTGNQWEIDYQQLGRHPEPRP
                     LNLDPGYLTDAKLVLATTKDRDHRIYLRDGIFAEVTLHFHRGRWEARPWTYPDYQRAD
                     YHAFFLRCRDFLRRR"
     gene            5045..>6110
                     /locus_tag="VFV87_10130"
     CDS             5045..>6110
                     /locus_tag="VFV87_10130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5104159.1"
                     /translation="MNFPLFIAGSVAPSLIIALLATFVVRRRAASWGLIDQPAERKVH
                     TTPTPRGGGLAIWLGVIGTFALGMVGLWLADRVPAIKDLVPDFAQPHLAGIWSQVGKL
                     WLLLSAASVMMLLGLMDDRGGLSWQLRLVVEFGVASVCVWMIDDLRLSAFIDLPLLMG
                     GLSVLWIVALVNSFNMLDNMDGLSGGVALISASMLAAVLLMAPDPTTNKPQLFVAGLL
                     LVVVGALAGFLWHNLPPAKIFMGDAGAYFVGFMIAIATLLASYASYKGELGERRHAIL
                     APLLVMAVPLYDMTTVILIRLRAGKSPFEADKNHFSHRLVDLGLTKTQAVLTVYLTTA
                     TCGLGALLLHMVDTTGAVIIM"
BASE COUNT         1153 a         1810 c         1949 g         1198 t
ORIGIN      
        1 cgaatgcctc cccctccgca tcgaccggcg gcagccgctc cagcacttcc cgtcggagct
       61 cctggttctc ccgttccagc gcctccaatc gctggcgaac gtcaaacaac tccgtggcga
      121 aatcctggcc gctggcgcac gccgcaatct gccacagcca gcaaccagcc acgagtagcg
      181 accagcgtcg catctccgct ccattggcgc gaggactcag ccccacccgc gcgtacggtc
      241 ggcgcggttg tatcgattgt gtcggacttg ccgtgcgtgt cgattgaacc ggttgctcga
      301 cggcgtcaaa tctaactcaa gttccccact ctacccattt ctccaacact tgcgacgaaa
      361 tctcggaagc gacagacaag tcgaattccg ctggtttgat tactgagacc gccagcgggc
      421 ggcaatttca tccgcatcca gcggcgtgac atccacgctc gggccccaga tcacgccgaa
      481 actcgcttgg cggatccgct cattgagagc ggcgatttcg tcgcggaccg cgggttcggt
      541 agcgagtgac ggcagttgag ccagcgtctg ctccacatcc agccggatcg ccagggcagg
      601 ggggagggcc gagagctgct cgcgcttgag cttgtccttg acccaccaca gttcgtcgtg
      661 gggctcgtcg attcccggga tcggctgccc aaagcccggc aggttatcga attcccccgc
      721 cgcctgcgcc gcccgtattc gctgctccgc gagcgaatcg aatggcaact tcgacttggg
      781 gatgtgatcg ggcatgttct cagtcgggac catacctggg cctagaccgg cgccggcgaa
      841 cccctgtcga tggtctctgg tcactcctag aatagcggcg cagccgcggc tatgcctggc
      901 caggcgttaa acgaaccacg cctctcaaat accgagtccc tggtcctcag accccagccc
      961 taaactccct ctgccatggc caaaacggca atcaccccga cgcgtcagga cgactatccc
     1021 gagtggttcc agcaggtgat ccgggccgcg gatttggccg agatctccgc ggttcgcggg
     1081 tgcatggtca tcaagccgtg gggctggtcg atctgggaga acatgcagcg ggtgctggac
     1141 ggaatgttca aggcgacggg gcacgagaat gcctatttcc cgctgttcat cccgctctcg
     1201 ttcctgcaga aggaggctga ccacgtcgag ggcttcgcca aggagtgtgc ggtcgtcacg
     1261 caccatcgcc tcgtgcccaa tagcgagggc aaactgattc ccgacccggc ttcgcagctt
     1321 gaggagccgc tgatcgtgcg gccgacgagc gaaacgatta tcggggcggc ctacgccaag
     1381 tgggtcgagt cgtatcgcga cctgccgatc ctcatcaatc agtgggccaa cgtcgtccgc
     1441 tgggagatgc ggacacggct gttcctccgc acggccgaat tcctctggca ggaggggcac
     1501 acggcccacg cgaccgccga ggaagcgcag gaagaaacga tgcggatgct cggcgtctat
     1561 gccgacttcg ccgagaacca catggcgatg ccggtgatcc agggcgagaa gacggcgggt
     1621 gagcggttcc ccggcgcggt tgcgacgtac gcgatcgaag cgatgatgca ggaccgcaag
     1681 gccctgcaat ccggcacatc gcacttcctc gggcagaatt tttcgaaggc ccaggaaatc
     1741 aagttccagg accagaatgg ccagcagcaa ttcgcctgga cgacgagctg gggcgtttcg
     1801 acgcgcctga tcggcggact gatcatgaca cattccgacg acgacggcct cgttctgccg
     1861 ccgcgtttgg cgccgaagca cattgtgctc ttgccgatct atcgcagcga cgacgagaaa
     1921 tcgcaggtgc tgccctacgt cgaatcgctg aagaaggagc tggaggcgca ggactatgcc
     1981 gatggcaagg tgcgggtgat gatcgacgac cgcgacatcc gcggcggcga aaagaactgg
     2041 caccacatca agcgcggcgt gccgctgcgg gcggaagtcg ggccgaaaga catcgccaaa
     2101 aacggcgttt tcctcgcccg ccgcgacacg agcgaaaaag caggcgtcga acggacgcag
     2161 ctcgtggcca cgattggcca gcggctgacg gaaattcagg acaacctctt ccagcgggcg
     2221 ctcaaattcc gccaggaaaa cacgcggacg atcgacaagc tcgacgactt cctggcctac
     2281 ttcacgccca aaaacgccga caatcccgaa atccacggcg gcttcgccct gtgccactat
     2341 tgcgaaggcc cggaaatgga cgagttcctc aagaagcaca aagtcacgat ccgctgcatc
     2401 ccgctgggcg gggcagggga ggggggcacg tgcttcttga ccgggaaacc tagtgccaaa
     2461 cgtgctgtct ttgcgaaagc ctactaactg ttcttagccg ggaccaaacc tggtcccggc
     2521 gattcgcgat tgagcacgaa catgtacgac ggcgatttgg aagagcactc ccagcaaggc
     2581 attcggtgga accagtcagt cgctctgttt ctcggtctcg tagccgtatt cggcctgggc
     2641 tacgctgccg ttgccctttg gcaagaggcg cgtttgccga acggtcgaga gctgtggcac
     2701 gggttagcgg tttcgtcggt actagtcgca gcgggtgcag ttgccatgtg gcggcgctca
     2761 ctcgtcgtcg gttttgttct tgtggtgttc gcgatggttt tctttggcgt atggatgccg
     2821 gaccggtagc aagattgaag ccactattcg actcgccggg accaggtttg gtcccggcta
     2881 aaagcaccgg ataggaatac tcctatgccc tgctaccact tcatcttcca cggtcacggc
     2941 acatggatgc ccgacgtaga cgacggttat gtcacgcgcg atgatggtca tctgccgcaa
     3001 gacacggcga tggcggcgcg gtatcgcgac ttgatggtcg aggaggaagt gacgttcacg
     3061 gaggaccagg agcagacgat gattgacgag gtccatgttg ctgcgggcaa acagaaattc
     3121 cgcgtacact acattgcgac cgagccgacg cacattcata ttttggtgag ttggaaggac
     3181 gaccgcccct tcgagaagct gcgggcgagc ataaggcaat cgatctcgcg gcggctgggg
     3241 aaggactacg agcggcggac gtggctgagc gagggcggga gccggcggaa ggtttcatcg
     3301 caggagcatt ttgattattt ggtggcgatt taccttccta agcatggcgg gtggaagtgg
     3361 agtgaggagc gggggttgtt ccggtgagga attcttggtg atggaaagtt aggtcgcagt
     3421 tcgccgggac caagtttggt cccggctagg aacaggttat ggcgctcatt cttcagacga
     3481 atcaacatta tttgcgaaat gcccagacgc tcggccatct ggccattgcc gcttatgcac
     3541 cgaagcccgc ggaggatgcg gcgttcaaga agacgatgtt tacggaggcg acctcgttcg
     3601 tgcatgggcc gacgagctcg ctggggtatt ggacggtgag cgacgatcat gtggtgctcg
     3661 cgttcgcggg gaccaatgac atccgcgact ggctcacgaa catccacttt gcgatggtgc
     3721 cggagctggg cggcaaagcg catcgcggct tcagccaggc gctcgatgca atctggagca
     3781 tcttcttcac gaagatcaag ctgcacaccg acgccggccg caagctgtgg atcaccgggc
     3841 acagcctggg cggggccctc gcgacgctcg ccgcccgccg gctgccgacg tcgatgaagc
     3901 ccgccggcgt ctataccttc ggccagccgc gggtgggcga cacgacgtac gccaagaagt
     3961 acaaactcaa gcaccatcgg ttcgtgcaca acaaggacat cgtgccgacg gtcccttcgc
     4021 ggttcattcc cggtgcattc ccgccggcgt tttacacgca cgtcggctcg ctcgagtttt
     4081 tcgacaagaa caagaagctc gtgggcagct catccgacga gttgggtttg atgcccgaac
     4141 tgcaggaggc cctgtcgcag ctttcgacaa cgacgactga ggctcaagcc aaagagctga
     4201 tcctcgatgg gattcgcgat cactcgatga aggcgtattt ggaatgtctg gcgaagaatg
     4261 tgccgtagag cggaactgcg gatttcgcgg acagcgcgga tgcggcaggc ggcaaatggc
     4321 ctcggatgga gcactgattt gcacgggtgc ggcacatgcg tagtcccatc cgcgttgtcc
     4381 gcgacatccg cggttcgatg cctcgatcgg accactgacg ttggcatggc gcacacacaa
     4441 ccccacatcg ctgttttgcg aatcctcgcg gcttttagcc gctacccgaa ggggctggac
     4501 tgggccaggc gaaccgcaga agccgcgtgg gggccagtcg ccttggaaag cgaacgtttc
     4561 gagcatcgtg agacgaacta ctatgacgcg acgatgggga cggacctcga gaagtgcttc
     4621 tttgctttcg aaacgctggc cgatccggct gcgcttgcgg aatggaagct gacgggtaat
     4681 cagtgggaaa tcgactatca gcagctcggc cggcatccgg agccacggcc gctgaatctg
     4741 gatccggggt atttgactga cgcgaagctg gtgctggcga cgacgaagga tcgggatcat
     4801 cggatttatc tgcgggacgg tattttcgcg gaggtgacgc tgcattttca ccgcgggcgg
     4861 tgggaagccc gcccgtggac ctatcccgat tatcagcggg ccgactatca tgcgttcttc
     4921 ctccgctgcc gcgactttct gcggcggcga taagtgttct tagccgggac caagcctggt
     4981 cccggtgagt cgggtgggat gtccactcgc agttctccgg gagcaagttt gctctcggcc
     5041 aggaatgaat tttcctctct tcatcgccgg ctccgttgct ccaagtttga tcatcgcgct
     5101 gctcgcgact tttgttgtcc gacggcgtgc cgcaagctgg ggtctgatcg accagccggc
     5161 ggagcgaaag gtgcatacca cgccgacgcc gcggggcggc ggattggcga tctggctggg
     5221 cgtgatcggg acgttcgccc tcggcatggt gggtctctgg ctggcagatc gagttcccgc
     5281 cattaaagat ctggtgcccg acttcgctca gccgcacctg gcaggcatct ggtcgcaggt
     5341 tggcaagctc tggctgctct tgtcggcggc gagcgtgatg atgctgctgg ggctgatgga
     5401 cgaccgcggc ggcctctcgt ggcaattgcg gctggtggtg gaattcggcg tggcgtcggt
     5461 ttgcgtctgg atgatcgatg acctgcgcct gtcggcattt attgacttgc cgctgctgat
     5521 gggcgggctg tcggtgctgt ggatcgtggc gctcgtcaac tccttcaaca tgctcgacaa
     5581 catggacggc ctctcggggg gcgtcgcgct gatttcggcg agcatgctgg cggccgtgct
     5641 gctcatggcg cccgatccga cgacaaacaa accgcagctc tttgtggcag ggctattgct
     5701 cgtcgtcgtc ggagcgctgg ccgggttttt gtggcataac ctgccgccgg cgaagatttt
     5761 catgggtgat gcaggggcct acttcgtcgg gttcatgatc gcgatcgcga cgctgctggc
     5821 cagctacgcc agctacaagg gtgagcttgg cgaacgtcgc cacgcgattc tcgccccgct
     5881 gttggtaatg gccgtcccgc tctatgacat gacgacggtc atcctgatcc gcctccgcgc
     5941 gggcaagagc cctttcgagg cggataagaa ccacttttcg caccggctcg tcgatttggg
     6001 gttgaccaaa acccaggcgg tgctgaccgt gtatctgacg acggccacct gcggcttggg
     6061 ggccttgctc ctgcacatgg tcgataccac gggcgcggtg attatcatgc
//
LOCUS       DASUGB010000526         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1864871, whole genome shotgun sequence.
ACCESSION   DASUGB010000526 DASUGB010000000
VERSION     DASUGB010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864871"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2128
                     /locus_tag="VFV87_10135"
     CDS             <1..2128
                     /locus_tag="VFV87_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5104160.1"
                     /translation="KRDFSSAEAYNKSIEPNRQRLAKILGVVDPRVPFDAPELVGTTK
                     QARSASEGHPLAVGKGENYDIFAVRWPAVRDVNGEGLLLVPKGKKVADIIAIPDADQT
                     PEQICGLAEGVPPESQFARRLAENGCRVLVPVLIDRKMQRRAPPGRQGGANLSNREYL
                     YRPAFELGRHLIGYEVQKVLAGVDWFAKEAGDKDAKIGVIGYGEGGMLALYAGALDTR
                     IDATCVSGYFSPRESIWEQPIDRNVFGLLEQFGDAELAAMICFRSLRIEADAYPVLTL
                     PSEGGAPAILKRPESESIESELSRRQMLATGVYPSFVAVVLKRGGEPPPFALSNATIR
                     GFFGDLLPKVTFAESGKLPEQLGTAPMREQRQQHEIEEDTQELLRESPFVREQFMKKL
                     YDCPSLEEYSKVVEEYREIFAKDVIGRFDYPLMEANPRSRKSYDTEKWVGYEVVLDVF
                     PDVFAYGILCLPKDLKPGEKRPVVVCQHGLEGRPQDTIGEQGSQYYSAFAGKLADRGF
                     ITFAPQNIYIGKDLFRTLQRKANPLGKTLFSIMVPQHQQIVNWLKEQPFVDGERIAFY
                     GLSYGGKSAMRIPPLVPDYCLSICSADFNEWVAKNASTRLPFSYVWTGEYEIFEFDLG
                     STFNYSEMAALICPRPFMVERGHTDGVSEDHWVAFEYAKVFFNYSHRLKIPDRCELEI
                     FDGPHKINGVATYAFLHKHLNWPEPK"
     gene            complement(2216..2401)
                     /locus_tag="VFV87_10140"
     CDS             complement(2216..2401)
                     /locus_tag="VFV87_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104161.1"
                     /translation="MRKIDHFYINGNASLGCGSRAVVSLDYNTVEIDLNHRVAANALT
                     DQIPLPVNARLLKFLPI"
     gene            2450..2809
                     /locus_tag="VFV87_10145"
     CDS             2450..2809
                     /locus_tag="VFV87_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104162.1"
                     /translation="MRCPPDVADELIEILKWGILQTRAWAFQKDDRRCVQETDHIHNL
                     PGLLNNYSPDLLAFYWNVERPLLIRQIGFEQCKAFHAAWEKLQGLVERECGSPQPDWF
                     AVPSGVAPLPTVEHAAK"
     gene            2825..>3456
                     /locus_tag="VFV87_10150"
     CDS             2825..>3456
                     /locus_tag="VFV87_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5104163.1"
                     /translation="MKSELTSSCGARLILVAPGSFILGSPPDEAGRAYWETEREVTLP
                     YEFYLGRAPVTQGQYERVTGENRTNHSAIADAPADSIGWQRATNFCAQLTQIDRDAGL
                     LSQDWEYRLPTETEWEYACRAGTSGPTHGPLDAIAWHFGNADQRPQPVCQKAPNAWGF
                     HDMLGNVWEVCQDWYSEKRQLRVGRGGSYFNTAKSCRAAARSPYAWGGRY"
BASE COUNT          703 a         1053 c         1068 g          632 t
ORIGIN      
        1 gaagcgggat ttctcctcag ccgaggcgta caacaagtcg atcgagccga accgccagcg
       61 attggcgaag attctgggcg tcgtcgatcc gcgggtgccg tttgacgcgc cggagctggt
      121 cggcacgacc aaacaagccc gaagcgcaag cgaggggcat ccgctcgccg ttggcaaagg
      181 tgagaactac gacatcttcg ccgtccgctg gccggctgtg cgcgacgtga acggcgaagg
      241 cttgctcctt gttcccaagg gcaaaaaagt cgccgacatc attgcgattc ccgatgcgga
      301 tcaaacgccg gagcaaatct gcggcctggc cgaaggcgtg ccgcccgaat cgcagttcgc
      361 ccgccggctg gccgagaacg gctgccgcgt gctggtgccg gtgctgatcg accggaagat
      421 gcagcgccgc gcaccacccg gccggcaagg cggcgcgaac ctgagcaacc gcgaatacct
      481 ctaccgcccc gccttcgagc tcggccggca cctgatcggc tacgaagtgc aaaaggtcct
      541 ggccggcgtc gattggttcg ccaaggaagc aggcgacaag gatgccaaga tcggcgtcat
      601 cggctacggc gaaggcggca tgctggccct ttatgccggc gccctcgaca cgcggatcga
      661 cgccacctgc gtcagcggct acttcagccc ccgagaaagc atttgggagc agcccattga
      721 ccgcaatgtg ttcggtttgc tggagcagtt cggcgatgcg gaacttgcgg caatgatttg
      781 ctttcggtcg ctacggattg aggccgacgc gtatccagtt ctcacattac cgagcgaagg
      841 cggagcccct gcgatcctga aaaggcctga atcggagtcg attgaatcgg aactctctcg
      901 tcgacaaatg ctggcaacag gtgtgtaccc ttcattcgtt gcggttgtcc ttaagagggg
      961 aggtgaaccg ccccctttcg cgctttcaaa tgctacgatt cgcggctttt ttggcgacct
     1021 cctaccgaag gtgacatttg ctgaatcggg aaaattgcct gagcagttgg gtacggcacc
     1081 catgcgcgag cagcgtcagc agcatgaaat cgaggaggac acccaagaac tcctccgcga
     1141 aagcccgttc gtccgcgagc agttcatgaa aaagctctac gactgcccgt cgttggaaga
     1201 atacagcaaa gtcgtcgagg agtatcgcga aatcttcgcg aaggatgtga tcggccggtt
     1261 cgattatccg ctcatggagg cgaatccccg cagccgcaag tcgtacgaca ccgagaaatg
     1321 ggtcggctac gaggtggtgc tcgatgtctt cccggatgtc ttcgcctacg gcatcctctg
     1381 cctgcccaag gacctgaagc cgggcgagaa gcggccggtg gtcgtgtgcc agcacggctt
     1441 ggaagggagg ccgcaggaca cgatcggcga gcagggctcg cagtattaca gtgccttcgc
     1501 cggcaagctg gccgaccgcg gcttcatcac gttcgccccg cagaacatct acatcggcaa
     1561 ggacctgttc cgcacgctcc agcggaaggc caacccgctc gggaagacgc tcttctcgat
     1621 catggtgccg cagcaccagc agatcgtgaa ctggctcaag gagcagccgt tcgtcgatgg
     1681 cgagcggatc gcgttctacg gcctgtcgta cggcggcaaa agcgcgatgc ggattccgcc
     1741 gctggtgccc gattattgcc tctcgatctg ctcggccgac ttcaacgagt gggtcgcgaa
     1801 gaacgcctcg acccgcctgc cgttcagcta cgtctggacg ggcgagtacg agattttcga
     1861 gttcgacctc ggcagcacgt tcaactacag cgagatggcc gccctgatct gcccgcggcc
     1921 gttcatggtc gagcgcggcc acaccgacgg cgtctccgaa gaccactggg tcgccttcga
     1981 atacgccaag gtcttcttca actacagcca ccggctgaaa atccccgacc gctgcgagct
     2041 cgaaatcttc gacggcccac acaagatcaa cggcgtcgcg acctacgcct tcctgcacaa
     2101 gcacctgaac tggccggagc cgaagtaggg gcgggcgcgg aacatgctgg cttttgatcc
     2161 cactcaagca gtgaagggag cggtgctgac gggcatcgat ccctcaacgc tgctttcaaa
     2221 tcggcaggaa cttgagcaat ctcgcattga ccggcaacgg gatttgatcc gtaagggcat
     2281 tcgccgccac acgatgattc aggtcaatct cgacggtgtt ataatccagg gaaaccacgg
     2341 cgcgcgaacc gcagccgagg ctggcgtttc cgttgatgta gaagtggtcg atcttccgca
     2401 tcctcattgg ggtccaatcc ttggaatccc cgttgttcgg aggtgaaaca tgcgatgccc
     2461 tcccgatgtg gctgacgaac tgatcgaaat tctgaaatgg gggattctgc aaacccgcgc
     2521 ctgggctttc cagaaggacg atcgccgttg cgtgcaggag accgaccaca ttcacaacct
     2581 cccggggctg ctcaacaact acagtcctga tctgcttgcc ttctattgga atgtggagcg
     2641 cccgttattg atccggcaga tcggctttga gcaatgcaaa gcatttcatg cggcctggga
     2701 aaagctgcag ggtcttgttg agcgcgaatg cggtagccct caaccagact ggttcgcggt
     2761 tccgtccggc gtcgccccgt tgccgactgt ggagcacgcc gcgaaatagc ctacgagacc
     2821 tcggatgaaa tcggaattga cgagttcctg cggcgcccgc ttgattctcg tcgctcccgg
     2881 ttcgttcatc ttgggaagcc cgccggatga agcggggcgg gcgtattggg aaacagaacg
     2941 ggaggtcacg ctgccctacg agttctatct gggcagggcg cccgtgacgc aaggacagta
     3001 cgaacgggta acgggcgaga accgaacgaa ccattcagca atcgccgacg cgcccgccga
     3061 ctcgattggc tggcagcggg caaccaactt ctgcgcccag ctcacccaga tcgatcgcga
     3121 tgccggcctg ctgagccagg attgggaata tcggctgccg accgaaacgg agtgggaata
     3181 cgcctgccgg gcggggacct ccggccctac gcacggcccg ctcgatgcca ttgcctggca
     3241 ctttggaaac gcggatcaga ggccgcagcc ggtctgccag aaggctccca acgcgtgggg
     3301 cttccacgac atgctcggca acgtctggga agtgtgtcag gactggtact cggagaaaag
     3361 gcagttgcga gtgggccgcg gcggcagcta cttcaacacg gccaagagct gtcgtgccgc
     3421 tgcccgctcg ccctacgcgt ggggcggccg ctacag
//
LOCUS       DASUGB010000527        10880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1447491, whole genome shotgun sequence.
ACCESSION   DASUGB010000527 DASUGB010000000
VERSION     DASUGB010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447491"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..706)
                     /locus_tag="VFV87_10155"
     CDS             complement(224..706)
                     /locus_tag="VFV87_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104164.1"
                     /translation="MAHDPRLFSPVFSIDVAADPRTAAAGTKIDANELIVGLLKQMVD
                     GQQRQIKLLEEIAHFVGLSHKQRQQELANWKEANPDLAKSCRTAAESLTVIQNDFLNK
                     LTDEAIDGHENMIESEYMLSEFVDRFGPRMAHLGGVLHMLSQLSAPKETPAAAPAEES
                     "
     gene            904..1428
                     /locus_tag="VFV87_10160"
     CDS             904..1428
                     /locus_tag="VFV87_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104165.1"
                     /translation="MSSTKPAACLGFLTVVEHADLGLLGGYLLLNPAGRPLEFHCTAP
                     LKANRTQEILYGPTLRPFLYGEQIGQTLLTRSKLRPLVVCTDSEPVLAAREFTHVPLV
                     WVEPEDRGQRTEDRNRFSLARNLVTAAPHYSSDEAAIRESWPAQADTLDLLEPFARIR
                     EALDEAQKSARAAA"
     gene            1515..3227
                     /locus_tag="VFV87_10165"
     CDS             1515..3227
                     /locus_tag="VFV87_10165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5104166.1"
                     /translation="MDLALPIASATTDFDAALSCPPPVALTPWHPNGPSPPQVASLKV
                     PRAAPRTFSFTFPEAPSARLPLAPSLRSSVSPSPPRPLAPARSSTRIKPPGDVIKLQD
                     RLYYVLQPPLETLIQSDALHFPFEPFPYQYQGVAFLYPRFSAILADEMGLGKTMQAIT
                     SIRMLLRSGEIRRVLLICPKPLVTNWKREFSLWAPEIPLTIVEGDAAKRQWQWTQENA
                     PVKIANYELLMRDRDVLDCNLHFDLVVLDEAQRIKNTGSATSQIARSISRTRNWALTG
                     TPIENTPDDLVGIFEFLSPGYLRPGLGARRMSELVKDHILRRTKDMVLKDLPPKLFRD
                     AELDLSPDQRATYEMAENEGVVHLSEMGDTLTIQHVFELVLRLKQICNFDPATGSSSK
                     LERLEADLEEVAASGKKAIVFSQWVKTLDTIAEKLGRFGPLFYHGQIPSKQRDGILKQ
                     FKEDPSKHVILMSYGAGSVGLNLQFCEYVFLFDRWWNPAVEDQAINRAHRIGAAGPVT
                     VTRMLALSTIEERINDVLNHKRELFDQIIGQTSAPASLGLSQAEIFGLFNLRTPKGAI
                     KSAA"
     gene            3511..3855
                     /locus_tag="VFV87_10170"
     CDS             3511..3855
                     /locus_tag="VFV87_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5104167.1"
                     /translation="MRLYVGNLAYGVTNESLEALFAQFGAVKSAQVVMDRDTGRSKGF
                     GFVEMADDNAARDAIKGLNETQHEGRPLTVNEARPREERGGGGGRGGGGGYGGGRGGG
                     GGYGGGGGGRRY"
     gene            complement(3950..4231)
                     /locus_tag="VFV87_10175"
     CDS             complement(3950..4231)
                     /locus_tag="VFV87_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104168.1"
                     /translation="MTQASPTNPYEAPREPVPRSLPDFSDREAAELADLRRRVMELEK
                     QVGNSWFVRGGVLRRVLAVWAYLLLGYGVMLAVVFGIMAIISAVTGEWP"
     gene            4306..5436
                     /locus_tag="VFV87_10180"
     CDS             4306..5436
                     /locus_tag="VFV87_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104169.1"
                     /translation="MAKSGGKQVNVAIIGLGFGAEFIPIYQAHPSANMYAICQRNEAK
                     LNKVGDSFGIETRYSEYADVLADPKVDFVHINSPINDHARMSIEALKAGKHVMCTVPM
                     ATTIKECDKICQLVDDTGLTYMMAETVVYSREFLFIKEMYKKGELGKIQHLAASHPQD
                     MDGWPDYWQKMIPMHYATHVVSPCLGLMDKRAEYVSCFGSGTVRKDIAKKSGNKFAVE
                     TCHIKLKDSDVTAHIWRFLYDVARQYRESFDVYGTKKSFEWTLVEHEPHVIHTAKKPE
                     PEIPSKVTVPDFAHLLPEPIRRFTQPAEIHDAQHLSFIQGGGHGGSHPHMVNEMVTAL
                     VKKRDPWPNATTAANWTCVGLCAHQSAEKGGKIVKLPAFTLE"
     gene            5447..6007
                     /locus_tag="VFV87_10185"
     CDS             5447..6007
                     /locus_tag="VFV87_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104170.1"
                     /translation="MLLRLATLHVALVLLAGCRAQQSQAPPTPPPEARPEVVVTSNEL
                     LAESPADAAEAIEPTRDRLAGNWEYKEGDKHLTLRFLQPGSGEGPLTRSRDGAARLDF
                     EGFGAVGSTTSDVVFDAANNQVVFYPPGGVDENGSPTVGPFATPEQAPKPWGKATLIG
                     NSLIKVDARIVGHWDWVLQTTFVKSQ"
     gene            6019..6900
                     /locus_tag="VFV87_10190"
     CDS             6019..6900
                     /locus_tag="VFV87_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104171.1"
                     /translation="MITNCLRRIIFCAAACMLLLSASGNAARADDAAKPLPKGQRVFY
                     TGHSFHMFVPQRIEQMVKAAKIEGHKLVGTQGIGGSRVIQHWNLADEKNKAKQALMTG
                     EVDVFTMAAHLQIPDEGITNFTELGLKHNPNLRLLVQESWYPFDGPPPDKNIKNNSQR
                     DDAKIADLQAAMDDWRKKFEAQADELNRKHGKTAVFIVPVGEAVVKLRGLILGGKYPG
                     VAKQSELFTDPIGHGGPHIQNLASYCNFAAIYRISPKGLNLPGLTDEQNAIQQKIAWE
                     TVSAYPYAGVASGNAEK"
     gene            6910..7350
                     /locus_tag="VFV87_10195"
     CDS             6910..7350
                     /locus_tag="VFV87_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase"
                     /protein_id="HEX5104172.1"
                     /translation="MRRIGNRALWIGPALNAAGLRMVHEQGIEALVDLAINEPVLQLT
                     RELIYCRFPLVDGAGNRPESLRIAVETTADLIRSGTPTLVFWSGGMSRSPAIAAAALS
                     VATGASLADCLAEVVAAGPKDLSPALWKDIGEVLGGMSGQAASR"
     gene            7417..>10880
                     /locus_tag="VFV87_10200"
     CDS             7417..>10880
                     /locus_tag="VFV87_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5104173.1"
                     /translation="MISSSAFVRVFSILLLACPVFPALAQPATEGQGEQPERFGAPPE
                     GYDRHREGAERGKIETVEYDSATVGIKRKARVYTPPGYTTDQKYPVLYLLHGIGGDEN
                     EWGRGGAPDAILDNLIAEKKAKPMIVVMPNGRASKDLTRRDPIPKQSPAFAAFEKDLL
                     TDLIPFIEKTYSVKADRESRALAGLSMGGGQSLNFGLSNLDTFAWVGGFSSAPNTRRP
                     ADLIQDHADAAKKLRLLYVACGDQDGLSRISQGVHEMLDEKQVPHVYRVIPGGRHDMR
                     VWRSDLYHFAQLIFREPEQVQETPQAKAAAEPLELLFLGDNGHHQPAARFKELAPVLK
                     ERGIELTYTDKLSDLNAETLGKYAGLVIYANHERISPEQEQALLEYVEGGKGLIPLHC
                     ASYCFLNSQKYVDLVGAQFQRHGTGTFRTIVAEGDHPILRGFGGFESWDETYVHTKHN
                     EKDRTVLEWREEGRAGGVSPLRKEPWTWVRTQGKGRVFYTAWGHDQRTWGNPGFLNLV
                     ERGIRWAVGDDPAVVPAFVDKRSATESVASSDFKPEMTPKRTDVKPFEYVKAKIAYYP
                     PGGRRSGDGEWNKMQLPLSPEESIKHFVTPVGFEMRLFVSEPQLEGKPIFMTWDERGR
                     LWVCETYDYPNELQPAGQGRDRIRICEDTDGDGQADKFTVFAEKLSIPTAIAFYRGGA
                     LVQAGGETLYLKDTDGDDRADLRKTLVTGWGMRDTHGEVSNFRYGLDNWYYAMQGYND
                     SRPILTDGTPVQSFRQGFFRFKIEGEGDKTAVTELEFLRSTNNNTWGLGISEEGIIFG
                     STANGNPSEYLPIPNRYYEKVRGWSSSVLGGIAESNRYYPITDKVRQVDFHGGFTAAA
                     GHALYTARNYPQEYWNRTAFVCEPTGHLVATFVIRPDGAGFKSKNAFNLVASDDEWCA
                     PIMAEVGPDGNVWILDWYNYIVQHNPTPQGFRTGKGAAYETDLRDKKHGRIYRLVPVG
                     QASSLPKNEVAPGASSTQPQSPFSAGKMPAPLTDATPEQLVETLKSDNMFWRLQAQRL
                     LVERGEKVVVPERLGRLIGDRNVDAVGLNPGAIHAVWTLKGLGELQGLRASLTPTANH
                     IEAVRTAQIDSSPGVRRNANQVIPPFRLGRLQSERDPQALLALLLAYADFYADASTAK
                     EAVNALENP"
BASE COUNT         2178 a         3479 c         3330 g         1893 t
ORIGIN      
        1 ggactcgggg atcggggctc gcgtgcgcgg gcgagcgcgc catctaatcg caggccgtcg
       61 cgagtaaccg tgcgaaccag ctcgacctgc atcgctcgtg gggcaggcat cgtgcctgcc
      121 aagaaagggt gcggcgggcg aatgaaagcc agcgcttttt cagcgcgaaa cgtcgcaggc
      181 tggaagccta cgccacggcg acaggctgaa agcccgcccc acgttacgat tcctcagccg
      241 gcgcggcggc gggcgtctcc ttcggggcac tgagctgcga cagcatgtgc agtacgccgc
      301 cgagatgggc catccgtggg ccgaagcgat cgacgaactc gctgagcatg tactcgctct
      361 cgatcatgtt ctcgtggccg tcgatcgcct cgtcggtcag cttgttcagg aagtcgttct
      421 gaataaccgt cagcgactcg gcagccgtgc ggcagctctt ggcgagatcc ggattggcct
      481 ccttccagtt ggccagttcc tgctgccgct gcttgtggct gaggccgacg aagtgggcga
      541 tttcctccag cagcttgatc tgccgctgct gtccatcgac catctgcttg agcaggccga
      601 cgatcagctc attggcgtca atcttggtcc ccgccgcagc cgtccgcggg tcggcggcca
      661 cgtcgatgct gaacacgggc gaaaacagac gcgggtcgtg ggccatgccg ctgggctcct
      721 tgtgattccc tcaatataca cactccgcgc caagtgtcga ggaattggcg aactgtgacg
      781 gtcgcatcga gaattccgta gccgatctct cacgagttcg gctacctgcg gctcaaggca
      841 gccgccacac cggttcgata gattcgccag tcaagccgct gccgccacct cgtcgccgcc
      901 ctcatgagct ccaccaagcc ggctgcctgt ctggggttcc tcaccgtcgt cgaacacgcc
      961 gacttgggcc tcctcggcgg ctatttgctg ctcaatcccg cgggccggcc gctggagttt
     1021 cactgcaccg ctcccctgaa ggccaatcgc acgcaggaaa tcctgtacgg tccgacgctg
     1081 cggccgttcc tttacggcga gcaaattggg caaactttgc tgaccaggtc aaagctgagg
     1141 ccacttgtcg tctgcaccga cagcgagccg gtgctggcag ccagggaatt cacgcatgtg
     1201 ccgctggtgt gggtcgagcc agaggacaga ggacagagga cagaggacag aaatcggttt
     1261 tcgctggcgc gaaatctggt gacggcggcg ccgcattatt ccagcgacga agcggcgatt
     1321 cgcgagtcgt ggccggcgca ggccgacacg ctggatctgc tggagccctt cgcgaggatt
     1381 cgcgaggcct tggacgaggc tcagaagtcg gcgagggcgg cagcgtgagc gccaggagtc
     1441 aggggtcagg agccagggaa gtacttatta ctcagtactt agtactgatc accgtgaagc
     1501 cgactgctac caccttggac cttgcccttc cgatcgcgtc ggccaccacc gacttcgatg
     1561 ccgccctttc ctgcccgcct ccagttgccc tcacaccttg gcatccaaac ggtccaagtc
     1621 caccccaggt tgcctccctc aaagtccccc gcgccgcccc gcgcaccttc agcttcacct
     1681 tccccgaagc accttccgcc cgtctccccc tcgcgccgtc tctccgttcc tccgtctccc
     1741 cctcgccccc tcgcccgctc gcccccgcgc gcagcagcac ccgcatcaag ccccccggcg
     1801 acgtcatcaa gcttcaggac cggctctatt acgtcctcca gccgccgctg gagacgctga
     1861 tccaatcgga cgcgttgcac ttcccgttcg agccgtttcc gtatcagtac cagggcgtcg
     1921 cgttcctcta tccgcgcttc tccgcgattc tggccgacga aatgggcctg gggaaaacga
     1981 tgcaggccat cacgtcgatc cgcatgctcc tccgctcggg cgagattcgc cgcgttctgc
     2041 tgatctgtcc caagccgctg gtgacgaact ggaagcggga gttctcgctg tgggcgccgg
     2101 agattccgct gacgatcgtc gaaggagatg cggccaagcg ccagtggcaa tggacgcagg
     2161 aaaacgcgcc agtcaagatc gccaactacg agctgctgat gcgcgaccgc gacgtgctgg
     2221 actgcaatct gcatttcgac ctggtcgtcc tcgacgaagc ccagcggatc aaaaacaccg
     2281 gcagcgccac cagccaaatc gcccgctcca tcagccgcac ccgcaactgg gccctcaccg
     2341 gcacgccgat cgagaacacg cccgacgacc tggtcggcat cttcgaattc ctctcgcccg
     2401 gctatttacg tcccggcttg ggggcgcggc ggatgagtga gctggtcaag gaccacatcc
     2461 tccgccgcac caaggacatg gtcctgaaag acctgcctcc caagctcttc cgcgatgctg
     2521 agctcgacct ttcgcccgat cagcgggcca cctacgaaat ggccgagaat gaaggcgtag
     2581 tacacctgag cgagatgggc gacacactga cgattcagca cgtcttcgag ctcgtcctga
     2641 ggctcaagca aatctgcaat ttcgaccccg ccaccggatc cagcagcaag ctggagcgcc
     2701 tcgaagccga cctggaagaa gtcgccgcca gtggcaagaa ggcgatcgtc ttcagccagt
     2761 gggtcaagac gctggacacg atcgccgaga agctcggccg tttcggcccg ctcttctatc
     2821 acggccaaat ccccagcaag cagcgcgacg gcatcctcaa gcaattcaag gaagacccgt
     2881 cgaagcacgt catcctgatg agctatggcg cgggcagtgt cggcctcaac ctgcagttct
     2941 gcgaatacgt cttcctcttc gaccgttggt ggaacccggc tgtcgaggat caggctatca
     3001 accgggccca tcgcattggg gctgccggac cggttacggt aacccgcatg ctggccctca
     3061 gcacgatcga agagcggatc aacgacgtgc tgaaccacaa gcgcgagctg ttcgaccaga
     3121 tcatcggcca aacgagcgcc cccgccagcc tcggcctctc ccaagcggag atcttcggcc
     3181 ttttcaacct tcgtacgccc aaaggagcga tcaagtcggc ggcgtagggg caggggtcag
     3241 gagccaggat tcaggagccg gcgctcccgg acaacaatcc gcaatccgaa atccgcaatg
     3301 atgccccatt gcatctagcc tcgcaccagc ccatccccta tactttcagg cggttggtag
     3361 gacgacgcca tttggagcgt tggttcctga ggaatctgcc cgctcgcttg tccctcgcct
     3421 ttcccacctg gcaggccgcc atgtttggcc ctttccttgg ccccgaaagt gaagcgggcc
     3481 gcatctgttc tgaggagtgt tcctgtggga atgcgtttgt atgtcggcaa cctggcttat
     3541 ggcgtgacca acgaatcgct ggaggccctg ttcgcccaat ttggcgccgt caaaagcgct
     3601 caggtcgtaa tggaccggga taccggtcgc agcaaaggct tcggcttcgt ggaaatggcg
     3661 gatgacaatg ccgcccgcga cgcgatcaag ggcctgaacg agacccagca cgaaggccgg
     3721 ccgctgacgg tcaacgaagc tcgcccccgc gaagagcgag gcggcggtgg tggccgaggc
     3781 ggcggcggtg gctacggcgg cggccgcggt ggcggcggtg gctatggcgg cggtggcggc
     3841 ggacgccgtt actaagcctc accagcttgc aacaactcga taaaccttgg ggccgggtcg
     3901 gcaattcgct gtcccggccc ttttgattgc gcgagcgggt ccaaatgaat catggccact
     3961 cgccagtgac ggccgagatg atcgccatga ttccgaatac cactgccaac atgactccgt
     4021 atccgaggag caggtacgcc cacacggcga gaactcgtcg caggactccg ccccgaacga
     4081 accacgaatt gccaacctgc ttttccaatt ccatgactcg ccgccgcagg tcggccagct
     4141 ccgcggcttc gcggtctgag aaatctggca gactccgcgg cacaggctcg cgcggcgcct
     4201 cgtagggatt cgtgggactg gcttgagtca tgccgggatt gtaccttggg cgaattccgg
     4261 attggcggca tccgcgcttc ttttctgcat caggagatgg gcaccatggc caaatcgggc
     4321 ggcaagcaag tcaacgtggc gatcattgga ctcggcttcg gggcggagtt cattccgatc
     4381 taccaggcgc atccaagtgc caacatgtac gcgatctgcc agcggaacga ggcgaagcta
     4441 aacaaggtcg gcgacagctt tggtatcgaa actcgataca gtgaatacgc ggacgtgctg
     4501 gccgacccga aggtcgattt cgtccacatc aattcgccga tcaacgacca cgcccggatg
     4561 tcgatcgagg ccctcaaagc cggcaagcac gtgatgtgta ccgtgccgat ggccacgacg
     4621 atcaaggagt gcgacaagat ctgccaactc gtcgacgata ccggcctgac gtacatgatg
     4681 gccgagaccg ttgtctatag ccgcgagttc ctcttcatca aggagatgta caagaaaggc
     4741 gaactcggca agattcagca cctggccgcc tcgcacccac aggacatgga cggctggccc
     4801 gactattggc agaagatgat cccgatgcac tacgccacgc acgtcgtcag cccgtgcctg
     4861 ggcctgatgg acaagcgagc cgagtacgtc tcctgcttcg gcagcggcac cgtgcggaag
     4921 gacatcgcca agaagtcggg caacaagttt gccgtcgaga cgtgccacat caagctcaaa
     4981 gattccgacg tgacggctca catctggcgg ttcctctacg acgtggcccg gcagtatcgc
     5041 gagagcttcg atgtctatgg cacgaagaag agcttcgaat ggacgctcgt cgagcacgag
     5101 ccgcacgtga tccacacggc caagaagccc gagccggaga ttccgagcaa ggtcacggtg
     5161 cccgacttcg cccacctctt gcccgagccg atccgccgct tcacgcagcc ggcggagatt
     5221 cacgacgccc agcacctctc gttcatccag gggggcgggc atggcggctc gcacccgcac
     5281 atggtcaacg agatggtcac tgcgctcgtg aagaagcgcg acccctggcc caatgccacc
     5341 accgccgcca actggacctg cgtcggcctc tgcgcgcatc aatcggccga gaagggtggt
     5401 aaaatcgtca agctgccggc gtttacctta gaataggatc gcttccatgc tcctgcgctt
     5461 ggcgactctt cacgttgcgt tggtcctgct ggctgggtgc cgcgcacaac aatctcaagc
     5521 gccacccacg ccaccacctg aggccaggcc ggaagtcgtt gtcacttcga acgaattact
     5581 tgctgaatcg cctgctgatg ctgcagaggc gatcgaaccg acgcgcgacc gcctcgcagg
     5641 caattgggag tacaaggaag gagacaaaca cttgaccctg cgctttctac agccaggcag
     5701 tggcgaaggg cctttgaccc gctcacggga tggcgcagcg cgactggatt tcgaggggtt
     5761 cggcgccgtc ggctcaacga ccagcgacgt cgttttcgat gccgcaaaca atcaggtcgt
     5821 tttctatccg cccggtggag ttgatgaaaa tggcagcccg acagtcgggc cgttcgcaac
     5881 accagaacaa gcgccgaaac cttggggcaa ggcaacgctg attgggaact cgctcatcaa
     5941 ggtagatgcc cgcatagttg ggcattggga ctgggtactg caaacaacgt ttgttaaatc
     6001 acaataggct aacgaaccat gatcacgaat tgtttgcggc gaatcatatt ctgcgctgcc
     6061 gcgtgcatgc tgttgctgag tgcgtcgggg aatgccgccc gcgccgatga cgcggccaaa
     6121 ccgctcccga aaggccagcg cgtcttctac accggccaca gcttccacat gttcgtcccg
     6181 cagcggatcg agcagatggt gaaggcggcc aagatcgagg gtcacaagct ggtcggcacg
     6241 caggggatcg gtggctcgcg ggtgattcag cactggaatc tggccgacga gaagaacaag
     6301 gccaagcagg cgctcatgac cggcgaggtc gatgtcttca cgatggccgc tcacctgcag
     6361 attcccgatg aggggatcac aaacttcacc gagctggggc tcaagcacaa cccgaacctg
     6421 cggctgctcg tgcaggagtc ctggtatccc ttcgacggcc cgccgccgga caaaaacatt
     6481 aagaacaatt cccagcgcga cgacgccaag atcgccgacc tgcaagccgc aatggacgac
     6541 tggcgcaaga agttcgaagc ccaagccgac gagctcaacc ggaagcacgg caaaacggcc
     6601 gtcttcatcg ttccggtcgg cgaagcggtc gtgaagctcc gcggcctgat cttgggcggc
     6661 aaataccccg gcgtcgccaa acaatcggaa ctcttcaccg accccatcgg ccacggcggc
     6721 ccgcacatcc aaaacctcgc ctcctactgc aacttcgcgg cgatttaccg catcagcccc
     6781 aaaggtctca acctgcccgg cctgaccgac gagcagaacg ccatccagca gaaaatcgcg
     6841 tgggagacgg tgtcggcata tccctacgcg ggtgtcgcgt ctggaaacgc cgaaaaatag
     6901 atccatacca tgcgccgcat cggcaatcgc gctctctgga tcggtccggc gctaaatgct
     6961 gccggacttc ggatggttca cgagcagggc atcgaggccc tggtggacct ggcgatcaat
     7021 gaaccggtgc tgcagctcac gcgggagctg atctattgcc ggtttccgct cgtggacggc
     7081 gcgggcaacc ggcccgagtc gctccgaatc gctgtggaaa ccactgccga cctgatccga
     7141 tccggcacgc ccacgctcgt gttctggagc ggaggcatga gccgctcgcc ggccattgcg
     7201 gcggcagccc tgtcggttgc gacgggtgcg tcgctcgcag actgtctggc tgaggtcgtg
     7261 gctgcgggcc caaaagacct gtcgccagcg ctctggaagg acatcggcga agtgctcggc
     7321 ggcatgtctg gccaagcggc gagtcgatag tgccaaatgc cctactgggg tattatggtc
     7381 tgatcccgag acttctcttg cccgaagacc atcgtcatga tcagttccag cgcgttcgtc
     7441 agggtttttt cgatcctcct gctggcatgc ccagttttcc cggcattggc gcaaccggca
     7501 accgagggac aaggcgagca gccggagcga ttcggcgcgc cgcctgaggg ttatgaccgc
     7561 caccgcgagg gcgccgagcg cggcaagatc gagacggtcg agtacgactc ggcgacggtt
     7621 ggaatcaagc gcaaggcgcg ggtctataca ccgccgggtt acacaaccga ccagaagtat
     7681 ccggtgctgt acctgctgca tggcattggc ggcgatgaga acgagtgggg gcggggcggc
     7741 gcgccggatg cgatcctcga taacctgatt gccgagaaga aggcgaagcc gatgatcgtc
     7801 gtcatgccca acggccgggc ctcgaaggac ctgacgcggc gggacccgat ccccaagcag
     7861 tcgccggcgt ttgccgcgtt tgaaaaggat ctgctcaccg acctgattcc gttcatcgag
     7921 aagacctact cggtgaaagc cgaccgcgag tcgcgggcgc tggccggtct ctcgatgggg
     7981 ggcgggcagt cgctcaactt cggcctgagc aacctcgata cctttgcctg ggtcggcgga
     8041 ttctcctccg cccccaatac caggcgtccg gccgacctca tccaggacca cgccgacgcg
     8101 gcgaaaaagc tgcggctgct ctacgtggcc tgcggtgatc aggacgggtt gtcgcgaatc
     8161 agccagggtg tccacgagat gctggacgag aagcaagtgc cgcacgtcta tcgcgtcatt
     8221 cccggcggcc ggcatgacat gcgcgtgtgg aggagcgacc tgtaccattt tgcgcagctc
     8281 atcttccgcg agccggagca agtgcaagaa acgccgcaag caaaggccgc cgccgagccg
     8341 cttgagctgc tcttcctcgg agataacggc caccatcagc cggcggcgcg gttcaaggaa
     8401 ctagcgccgg tgctgaagga gcgggggatc gagctgactt atacggacaa gctcagcgat
     8461 ttgaatgcgg agacgctggg caagtacgcg gggctggtga tctatgccaa tcacgagcgg
     8521 atttcgcccg agcaggagca ggcgctgctc gagtatgtcg aaggcggcaa ggggctgatt
     8581 ccactgcact gcgcgtcgta ttgcttcctg aattcgcaga aatacgtcga tttggtcggc
     8641 gcacagttcc agcggcatgg caccgggacg ttccgcacga tcgttgcgga aggcgaccac
     8701 ccaatcctcc gcggctttgg cgggttcgag agctgggacg agacctatgt gcatacgaag
     8761 cacaacgaga aggaccggac cgtgctggaa tggcgtgaag aagggcgagc ggggggcgtg
     8821 agccccctga ggaaagaacc ctggacctgg gtccgcacgc agggcaaggg gcgcgtgttc
     8881 tacactgcct ggggccacga ccagcggacc tggggcaatc cgggattcct caatctggtt
     8941 gaacgcggca ttcgctgggc cgtcggcgac gatccggctg ttgtgcccgc gttcgtggac
     9001 aagcggagtg ccacagagag cgttgcctct agcgacttca agccggaaat gacgcccaag
     9061 cggaccgacg tcaagccgtt cgagtacgtc aaggcgaaaa tcgcctacta tccgcccggc
     9121 ggccggcgga gcggcgatgg cgagtggaac aagatgcaac tgccgctttc gcccgaggag
     9181 tcgatcaagc actttgtcac acccgttggc tttgagatgc gtttgttcgt cagcgagccg
     9241 cagctcgaag gcaagccgat ctttatgacc tgggacgagc gcggccggct gtgggtgtgc
     9301 gagacgtacg actatccgaa cgagctgcag ccggcgggcc aggggcgcga ccggattcgc
     9361 atttgcgaag acacggacgg cgacggccag gcggacaaat tcaccgtgtt cgccgagaag
     9421 ttgagcattc ccaccgccat cgccttctac cgcggcggag ccctcgtcca agccggcggc
     9481 gagacgctgt atctcaagga caccgacggc gacgaccggg ccgacctgcg gaagacgctc
     9541 gtcaccggct gggggatgcg cgacacgcac ggcgaggtga gcaacttccg ctacggcctc
     9601 gacaactggt actacgccat gcagggctac aatgactcgc ggcccattct gaccgatggc
     9661 acgccagtgc agtcgttccg ccaaggcttt ttccggttca agatcgaagg cgaaggggac
     9721 aagaccgcgg tgacggagct agaattcctc cgctcgacga acaacaacac gtggggcctg
     9781 gggatcagcg aggaaggcat cattttcggc tcgacggcca acggcaaccc gagcgagtac
     9841 ctgccgatcc ccaaccgcta ctacgaaaag gtccgcggct ggtcgtcgtc ggtgctgggg
     9901 ggcatcgccg agagcaatcg gtattaccca attaccgaca aagtccggca ggtcgatttc
     9961 cacggcggct tcaccgcggc cgcgggacac gcgctctaca cggcccgcaa ctatccgcag
    10021 gagtattgga accgcaccgc cttcgtgtgc gagccgacgg ggcacctggt cgcgacgttc
    10081 gtgatccggc cggacggcgc gggcttcaag tcgaagaacg cgttcaacct cgtcgcgagc
    10141 gacgacgaat ggtgcgcccc gatcatggcc gaggtcggcc ccgacggcaa cgtctggatt
    10201 ctcgattggt acaactacat cgtccagcac aatcccacgc cgcaaggctt ccgcacgggc
    10261 aagggcgccg cctacgaaac cgacctccgc gacaaaaaac acggccgcat ctaccggctg
    10321 gtccccgtgg ggcaggcttc cagcctgcca aagaacgagg ttgccccagg cgccagctca
    10381 acccaaccgc aaagcccatt ctcggcaggc aagatgcctg ccccactgac cgacgccact
    10441 cccgaacaac tcgtcgaaac gctcaagagc gacaacatgt tctggcggct gcaagcacag
    10501 cggttgctgg tcgaacgcgg cgagaaggtc gttgtgccag agcgcctagg aaggctaatc
    10561 ggcgatcgca atgtagatgc tgtcggtctc aatcccggcg cgattcatgc agtgtggacg
    10621 ctcaagggct taggcgagtt gcaggggctc cgggcctcat tgacgccgac cgcgaaccat
    10681 atcgaggccg tgcgtaccgc ccagatcgac tcatcgccgg gagttcggcg aaacgcaaat
    10741 caagtgattc ccccattccg tctcggtcgc ctccagtcgg agcgagaccc gcaggctctg
    10801 ctcgcgctgc ttctcgcgta cgccgatttc tacgctgatg catctacagc caaggaagct
    10861 gtgaatgcac ttgaaaatcc
//
LOCUS       DASUGB010000528         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_614512, whole genome shotgun sequence.
ACCESSION   DASUGB010000528 DASUGB010000000
VERSION     DASUGB010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614512"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFV87_10205"
     CDS             complement(<1..326)
                     /locus_tag="VFV87_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEX5104174.1"
                     /translation="MRILLIGDIVGKPGVAIVTRALAGLRRREGLDLVIANAENAADG
                     SGLSPSIYRKLVAAGVDCVTLGDHIYRRSEIAGVLNSESNIVKPANYPQQAPGRTWAV
                     VRAGEGT"
     gene            complement(415..1950)
                     /gene="rny"
                     /locus_tag="VFV87_10210"
     CDS             complement(415..1950)
                     /gene="rny"
                     /locus_tag="VFV87_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5104175.1"
                     /translation="MAVAVVVAAGLTFAVVKFLDRLRKVDIENLAKQITEKAERDAAT
                     KLKEADLAIKERDLAQKAAADKELAKSRDEIRERERALDKRQEVLTQQDDDLRKQQKM
                     VESNQRRLAERMDDVNRRNEELVKILEMQRQSLHSISGLSPGDATKRLLDSLEQELSQ
                     EMGSVLIRHEKRVAELVDQKARVMLLTAMQRYAAAHTAESTTSTVDIPSDEMKGRIIG
                     REGRNIRTFEKVTGVDVIIDDTPGVVIVSGFDPVRREVARQTLNKLIADGRIHPSRIE
                     EVFTATEKEIEGFIQKKGEEATQEVNIHGVHPRVQYMLGRLHFRTSYSQNVLRHSIEV
                     AFLTGMLAEMIGMDGDLARRCGLLHDIGKAADHELEGGHPKIGADLLKRHGEKTEVVH
                     AAFGHHDEIVTDYPYTMLVATADACSASRPGARRESLERYIKRMEELESIACGFPGVE
                     QAFAIQAGRELRVIASARDTDDSRAAKICHDIAKAFEEQLTYPGEIKVTVVRESRFSE
                     VAR"
     gene            2276..2596
                     /locus_tag="VFV87_10215"
     CDS             2276..2596
                     /locus_tag="VFV87_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104176.1"
                     /translation="MLLHPAIGAGGHRKAVAPSGKEPAQHQTQNHQDQVHHATSIRSI
                     PEARTVRPAPPAEKPLDAARNGFINELTNRGWPQNRPAATTRHDRSMMDVRKCRRHAA
                     DGSL"
     gene            2687..>3329
                     /gene="tilS"
                     /locus_tag="VFV87_10220"
     CDS             2687..>3329
                     /gene="tilS"
                     /locus_tag="VFV87_10220"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5104177.1"
                     /translation="MLGVPGLRGGGVLISKLATDWPPDEWRDVTVLVAVSGGADSVAL
                     LRGLHQLKAAGEGRLVLAHFNHRLRGAESDADQALVEELARRLGLEAVVGARGREGEG
                     ERGRSEESLRELRYEFLAAAADKVGARFVATAHTADDQVETVLMNVLRGTGLAGLAGI
                     PRVRRLTDAATLIRPLLNVTRAEVLEYLHSLGQSYRDDATNQSADYTRNRVRLQ"
BASE COUNT          540 a         1077 c         1091 g          621 t
ORIGIN      
        1 gtcccctctc ctgccctgac gacggcccaa gtgcgccccg gcgcttgctg cggatagttg
       61 gccggcttga cgatgttgct ctcggaatta agcacgccag cgatctcgct gcggcgatag
      121 atatggtcgc ccagcgtgac gcaatcgact cctgcggcga ccagcttgcg atagatgctg
      181 ggcgacagcc ccgagccatc cgcggcgttc tcggcgttcg cgatcaccag gtccagcccc
      241 tcacggcggc gcaaaccagc cagcgcgcgc gtcacgatcg caacgcccgg tttgccgacg
      301 atatcgccga tgaggaggat gcgcattctt gtttttagcc gggagcaaac ctgctcccgg
      361 agaactgtga atcgacgtcg gtacccgcat cgccgggacc aggtttggtc ccggctatcg
      421 ggccacttcg ctgaacctcg actcccgcac caccgtgacc ttgatctccc ccggatacgt
      481 gagctgctct tcgaacgcct tggcgatgtc gtggcagatc ttggcggcgc gggagtcgtc
      541 agtgtcgcgg gcgctggcga tcacccgcaa ctcgcggccg gcctggatcg caaacgcctg
      601 ctcgacccct ggaaagccgc aggcgatcga ctccagctct tccatccgct tgatgtagcg
      661 ctcgagcgat tcgcgccgcg cgccggggcg cgaggcgctg caggcatcgg cggtggcgac
      721 gagcatcgtg tacggataat cggtgacgat ttcgtcgtgg tggccgaagg cggcgtggac
      781 gacttccgtc ttttcgccgt gccgcttgag caggtcagcg ccgatcttgg gatgcccccc
      841 ttccagctcg tggtcggctg ccttgccgat gtcgtgcaac aggccgcagc ggcgggcgag
      901 gtcgccgtcc atgccgatca tttcagccag catgccggtg aggaaggcga cctcgatgct
      961 gtgccgcagc acattctggc tgtagctcgt gcggaaatgc agccggccga gcatgtactg
     1021 cacccgcggg tggacgccgt gaatgttgac ctcctgcgtg gcctcctccc ctttcttctg
     1081 aatgaagcct tcgatctcct tctcggtggc ggtgaacact tcctcgatcc gcgaagggtg
     1141 aatgcggcca tcggcgatca gcttgttgag cgtctggcgg gcgacttcgc ggcgaaccgg
     1201 atcgaagcca ctgacgatca ccacgccggg cgtgtcgtcg atgatgacat cgacgccggt
     1261 gaccttctcg aacgtgcgga tgttccgccc ttcgcggccg atgatccggc ctttcatctc
     1321 gtcgctgggg atatcgaccg tgctggtcgt gctctcggcc gtgtgtgcgg ctgcgtatcg
     1381 ttgcatggcc gtcagcagca taacccgcgc cttctgatcg acaagctccg ccacgcgctt
     1441 ctcgtggcgg atgaggaccg agcccatttc ctgcgatagc tcctgctcga gcgaatcgag
     1501 caggcgcttg gtggcatcgc ccggcgaaag gccgctgatc gagtgcagcg actgccgctg
     1561 catctcgagg attttcacca gctcctcgtt gcggcggttg acgtcgtcca tccgctccgc
     1621 cagccgccgc tggttcgatt cgaccatctt ctgctgcttg cggaggtcat cgtcctgctg
     1681 cgtgagcact tcctggcgct tgtcgagggc ccgctcccgc tcgcggattt cgtcgcggga
     1741 cttggccagc tccttatccg cggcggcttt ctgggccagg tcgcgctcct taatcgccag
     1801 gtcggcttct ttgagcttcg tggcggcgtc ccgctcggcc ttttcggtga tctgtttggc
     1861 cagattctcg atatcgacct tccgcagccg gtcgaggaat tttacgacgg caaaggtcag
     1921 cccggccgcg acaacgaccg cgacggccat gacgccatat tcaaaagcac ccacgcgatt
     1981 cctcctgata tcggcaggcg atcgccggaa gtggatacag cgtcagacca acgcgctaga
     2041 ccaggcgcga caagcagcga ctcagccgcg gcccagccgc tcgcgatgaa cgcggacgca
     2101 agaaatgcgc gcaagttgtc cttgggagag gctaggttcc cggcgagggc ggcggagacg
     2161 cggttctgca gcgcccgggc gcggaccttc ggccgcgagc ccaaggagca cccggccctg
     2221 gcccatagcg gcgatcgttc gaatcgccga acagccaggt ccagaggctg tgcggatgct
     2281 gctccatccg gccatcggtg cgggcggcca cagaaaggcc gtcgcgccgt ccgggaaaga
     2341 gcccgctcag caccagacgc agaatcatca ggatcaggtt catcacgcca cttccattcg
     2401 gtccataccc gaagcaagaa cagttcgtcc ggccccgccc gcagaaaagc ccttggacgc
     2461 ggcccgcaac ggtttcatca acgagctaac caaccgcggc tggccgcaaa atcgcccggc
     2521 cgcaaccacc cgtcacgatc gcagtatgat ggatgtacgc aagtgccgcc ggcacgccgc
     2581 cgacggctct ttgtaagtcg tatcctacca gagtcttccg taacttcaac cattcggtgc
     2641 gacaatctga caccgtaggc atcttgctcc gcgggatgca gccggcgtgt tgggcgtgcc
     2701 gggcctgcgc gggggcggag tgctgatttc gaagctcgct actgattggc ctcccgacga
     2761 gtggcgcgac gtaacggtac ttgtcgccgt cagcggcgga gcggatagcg tcgcgctcct
     2821 tcgcgggctg catcagttga aagccgcggg cgagggccgg ctggtgctgg cgcatttcaa
     2881 tcaccgcctg cggggggcgg agtccgacgc cgatcaagcg ctggtggagg agcttgcgcg
     2941 gcggttgggg ctggaggctg ttgtaggcgc gagggggaga gagggagagg gggagagggg
     3001 gagatcggag gagtcgctgc gcgagttgcg gtatgagttc ttggccgcgg cagccgataa
     3061 agtcggcgcc cgctttgtgg cgacggcgca tacggctgac gaccaggtgg agacggtgct
     3121 gatgaacgtg ctccgcggca cgggtctggc cgggctggcg gggattccgc gcgtgcggcg
     3181 gctgaccgat gccgcgacgc tcatccggcc gctgctaaac gtcacgcggg ccgaggtgtt
     3241 agagtatctc cactcgctcg gccagtcgta tcgcgacgat gcgaccaatc agtccgccga
     3301 ctacacccgc aaccgcgtgc gcctgcagc
//
LOCUS       DASUGB010000529         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2005119, whole genome shotgun sequence.
ACCESSION   DASUGB010000529 DASUGB010000000
VERSION     DASUGB010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005119"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="VFV87_10225"
     CDS             <1..1829
                     /locus_tag="VFV87_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5104178.1"
                     /translation="CLAAVRASRAQEANQPESYIRLHYTKQEHMIPMRDGTKLFTAVY
                     LPKDTTKEYPILIKRTPYSCSPYGVDKYPSSLGPSEHFVKAGYIFVNQDVRGRFLSEG
                     EFVQVTPHVANKQKPTDVDESTDASDTIEWLIKNVPNHNGRAGMYGISYPGFYSAAGM
                     IDAHPALKAVSPQAPVGDWYFDDFLHNGAFFLAHAFRWLGNNAQTRGRPTSDKPKPVE
                     YRTFDGYELFLKAGTMDAVNKQLIKDAVPFWNEMMQHPNRDEFWQKRAILPHLKNVAP
                     AVMVVTGWYDAEDLYGSFKTYQSIESLNPKVNNVLVVGPWQHGGWASVDGDKLGSANF
                     GSKTAAFYRAEIELPFFEKYLKDAEKPTPAEATVFETGSNSWRTFDDWPPREAKATQF
                     FLQEGGLLDADPPSSVKEAFDEYVSDPAKPVPFTERITPMMAVEYMTDDQRFAARRPD
                     VLVYQTPPMESDLVVAGPLDVDLWVSTTGTDADFIVKLIDVFPDASEAAAQPNYQMLL
                     RSEVFRGRYRNSFDKPEPFVPGEPARLKFELLDVLHRFQKGHRLMIQIQSTWFPLVDR
                     NPQKYVPNIYFAKPEDFQKATHRIYRSAEYPTNIRFGSVPSP"
     gene            complement(2164..>3121)
                     /locus_tag="VFV87_10230"
     CDS             complement(2164..>3121)
                     /locus_tag="VFV87_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5104179.1"
                     /translation="EAAVLAGLQWLKKQQMQDGGWSLTGPYPEGAGVENRCSATAMAL
                     LAFQGAGHTHQSGEFKKEVLRGWDFLLKMQNADGYFEHEAAHHHRLYAQAQATIAVCE
                     IFGMTKDEKFKKAAQNALDFAHKAQAPEGGWRYEPKIDSDTSVTGWYVMALQSGRMAG
                     LEVQSPAEDSINKFLDRCTDNGSHYSYKPGMEQTLTMTAEAMLCRQYLGWKHDDERMR
                     QGINYLLANTVNYGDQNVYYWYYATQACHHMDGDDWNQWNRVMRQAVPEAQTKTGNDK
                     GSWSPSADRWGAHGGRLYVTCLSIYMLEVYYRHLPIYKWRLQ"
BASE COUNT          600 a         1018 c          913 g          590 t
ORIGIN      
        1 cctgcctcgc cgccgtccgc gccagccggg cccaggaagc caaccagccc gaaagctaca
       61 tccgcctgca ctacaccaag caggagcaca tgatcccgat gcgggacggc acgaagctct
      121 tcaccgccgt ctatctgccg aaggacacga cgaaggagta tcccatcctc atcaagcgca
      181 cgccctattc gtgcagcccc tatggcgttg ataagtaccc gtcttcgctc ggccccagcg
      241 agcactttgt gaaagccggg tacatcttcg tcaaccaaga tgtccgcggc aggttcctca
      301 gcgaaggcga gttcgtccag gtgacgccgc acgtagcgaa caaacagaag ccgaccgatg
      361 tggacgagag caccgacgcc tccgacacga tcgagtggct gatcaagaat gtgccgaacc
      421 ataacggccg cgccggcatg tatggcatct cgtatcccgg cttttattct gctgcgggga
      481 tgatcgacgc ccaccctgct ctcaaagccg tctcgccgca ggcgccggtg ggcgactggt
      541 atttcgacga cttcctgcac aacggggcct ttttcctggc ccacgccttc cgctggctgg
      601 gcaacaacgc ccagacccga ggcaggccga cctccgacaa gcccaagccg gtcgaatacc
      661 ggacgttcga cggctacgag ctgtttctca aagccggcac gatggacgcc gtgaacaagc
      721 agctcatcaa ggacgccgtg ccgttctgga acgagatgat gcagcatccc aatcgcgacg
      781 aattctggca gaagcgggcg atcctgccgc acctgaaaaa cgtcgccccc gccgtgatgg
      841 tcgtcaccgg ctggtacgac gccgaggacc tctacggctc gttcaaaacg taccaatcga
      901 tcgagtcgct gaacccgaag gtcaacaacg tgctggtcgt cggcccgtgg cagcacggcg
      961 gctgggcttc ggttgatggc gacaaactgg gcagcgccaa tttcggctcg aagaccgccg
     1021 cgttctaccg ggccgagatc gagctgccgt tcttcgagaa gtacttgaaa gacgccgaga
     1081 agccgacgcc ggccgaggcg accgtcttcg agaccggctc aaattcctgg cggacgttcg
     1141 acgactggcc gccgcgcgaa gcgaaggcca cgcagttctt cctgcaagag ggcggcctgc
     1201 tcgatgcaga cccgccgagt tcagtgaagg aagcgttcga cgaatatgtc agcgatccgg
     1261 ccaagccggt gccgttcacc gagcggatca cgccgatgat ggcggtcgag tacatgaccg
     1321 acgaccagcg gttcgccgcc cgccggccgg acgtgctggt ctatcagacg ccacccatgg
     1381 aaagcgacct ggtcgtcgca ggaccgctcg acgtggacct ctgggtatcg accaccggca
     1441 ccgacgccga tttcatcgtc aagctgatcg acgtctttcc cgacgcctcg gaagccgccg
     1501 cccagccgaa ctaccagatg ctcctccgca gcgaagtctt ccgcggccgg taccgcaaca
     1561 gcttcgacaa gcccgagccg ttcgttcccg gcgagccggc gcgcctcaag ttcgagctgc
     1621 tcgacgtgct gcaccgcttc cagaaagggc accggctgat gatccagatc cagagcacct
     1681 ggttcccgct cgtcgatcgc aaccctcaga agtatgtgcc caacatctat tttgccaagc
     1741 ccgaggattt ccagaaggcc acgcatcgca tatatcgctc ggcggagtat cccacgaaca
     1801 tccgcttcgg ctctgtgcca tctccttaag gccctcgcac atggcaaagc ccaagagttt
     1861 tcaggtgtgg aaacaaggca ggtctacagc ctgcgtgctg cagaggccac cccagccccg
     1921 cgagtcgctg gaagtgattc tggatgcgcc gattgaagtg gtcgttcgat tggcgcgggc
     1981 aggacgaaaa ctcattttcg ccttcgccgc agctgccaaa gtctgaggga cgtgccctct
     2041 aacgtttggc ttccacggta cgcgagaact tgctccgtat tgtcgcgcaa acgcgacgac
     2101 gtctccgcaa agaggagttc ggagcaatgg caggcgagac gccttcccac acgttatgat
     2161 tgcctactgc agccgccact tatagatggg caggtggcgg taatagacct ccagcatgta
     2221 gatcgagagg caggtgacgt agagccggcc gccgtgggcg ccccagcggt ctgcagaggg
     2281 agaccagctt cccttgtcat tgccggtttt cgtttgggcc tccggcaccg cctgccgcat
     2341 cacgcggttc cactggttcc agtcgtcgcc atccatgtgg tggcaggctt gcgtggcata
     2401 gtaccagtaa tagacgtttt gatcgccgta attgaccgtg tttgccagca ggtaattgat
     2461 gccctgcctc atccgttcgt cgtcgtgctt ccagccgaga tactggcggc agagcatcgc
     2521 ctcggcggtc attgtgagcg tctgctccat gcccggcttg tacgaatagt gcgagccatt
     2581 gtcggtgcaa cgatcgagaa acttgttaat cgaatcttcc gccgggctct gcacttccag
     2641 gcccgccatg cggccgctct gcaaggccat cacgtaccag cccgtgacgg aggtgtcgct
     2701 gtcgatcttg ggctcgtagc gccagcctcc ttcgggagcc tgcgccttat gggcgaagtc
     2761 gagtgcgttc tgcgccgctt ttttgaactt ctcgtccttg gtcatcccga agatctcgca
     2821 cacggcaatc gtggcctgag cctgggcata caggcgatgg tggtgggccg cctcgtgctc
     2881 gaaatagccg tcggcgttct gcatcttgag caggaaatcc cagccgcgca gcacttcctt
     2941 cttgaattca cccgactggt gtgtgtggcc ggcgccttgg aaggctaata gggccatcgc
     3001 cgtggccgag cagcggttct cgacccccgc accctccggg tagggtcccg tcaggctcca
     3061 accgccgtcc tgcatctgct gcttcttcag ccattgcagg ccggccagca cggcggcctc
     3121 g
//
LOCUS       DASUGB010000530         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_754446, whole genome shotgun sequence.
ACCESSION   DASUGB010000530 DASUGB010000000
VERSION     DASUGB010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754446"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFV87_10235"
     CDS             <1..1497
                     /locus_tag="VFV87_10235"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5104180.1"
                     /translation="LVMAAPAIVLLIPSRAAIGGDAASPLEEGKALFERRFAAGDAPA
                     LTGDGLGPVFNNVSCAACHLQAGVGGGGPIDVNAVILTAQLAGTRPKRDKLVEALREL
                     HPGFLTTDGKIVPSVLLHRFGTDLNYAGFLRGLGAPYVPEVPDRAQQDELVYQLAQQP
                     LPTATTAPPIRLTLSHRNTTALFGAGLIDQVPDSVLHAQAAAQTKQGEVSGRVPPVGI
                     DFVGRFGWRGQTERLHDFVLGACANELGLEVPGIPQPRNPLQPDYRPAGLDLTAAQCA
                     SLTSFVASLPPPKLLPPDEPAKRAVFDRGQVLFHSVGCASCHVEKIGPLDHVYSDLLL
                     HDMGPALADPLLAAPALRVIKQTPLEQSDAELLLGSTQPVSSQPKLPQPQLPPPRGYY
                     GESSSSSFALLGEEGPATITVVDRKAGVKEELRAVVSPLDREWRTPPLWGLADSAPYL
                     HDGRAATVVEAIAFHAGEADACTKRYFALPAGDRMAVLQFLACLQAPK"
     gene            complement(2414..3199)
                     /locus_tag="VFV87_10240"
     CDS             complement(2414..3199)
                     /locus_tag="VFV87_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104181.1"
                     /translation="MRTNRVVLLAALALAVPAVGMWVDEYKSGIVWPEPPVVDPGEAG
                     QPPADAVVLFGGENLDAWEAGDKWQIADGVATAAGGSITSQQKFGDCQVHLEFATPAE
                     VKGSGQGRGNSGLYLMGRYEVQILDSFDNQTYFDGQCGAIYKQQPPTVNASRRPGEWQ
                     SLDILFTAPRFHDDGTLKSPGYVTVLHNGLVIQNHFELQGSTSYTEPPKYTAHADKEP
                     LSIQFHGNPVRFRNIWVRENVQPLVGLKPDPQAPPQVKASLER"
     gene            3362..>4084
                     /locus_tag="VFV87_10245"
     CDS             3362..>4084
                     /locus_tag="VFV87_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5104182.1"
                     /translation="MKPSRLLALLLFSVSSVCCVVPLTAAELATQLRRPIALVQSADG
                     RQLFVANRDSGSVSVIDIGERQVVAEQAIGKRLADLAALPGSDRLLAIDEAAHELLLL
                     QPGAEGLEIIQRLPVSPYPVTICVLPGGKQATIASLWSQRLTFVELGKTLKIDSVLDL
                     PFAPRCQLVLQNDGRRSMPATLLVADSFSGRLALIDPANRKLTATREFPGHNVRGLGA
                     SPDGRMLIVAHQMLNELAHSIRN"
BASE COUNT          646 a         1455 c         1220 g          763 t
ORIGIN      
        1 ctcgtcatgg cagcgcccgc gatcgtgttg ctgatcccgt ccagggcggc aatcggaggc
       61 gatgccgcca gcccgctcga ggaaggcaaa gcgctcttcg agcggcggtt cgccgcgggc
      121 gacgcgccgg ccttgaccgg cgacggcctg gggcccgtgt tcaacaacgt ctcctgtgcc
      181 gcttgccacc tgcaggcggg cgtgggcggc ggcggaccga ttgatgtcaa cgccgtcatc
      241 ctcacggctc agctcgccgg cacgcggccc aagcgcgaca agctcgtcga agccctgcgc
      301 gagctgcacc cgggctttct caccacggac ggcaagatcg tacccagcgt tctgctccat
      361 cgcttcggaa cagatctgaa ttacgccggc ttcctccgcg gattgggcgc gccgtacgtc
      421 ccggaagtgc ccgaccgggc ccagcaggac gaactcgttt atcaactcgc ccagcagccg
      481 ctgccgacgg cgacaaccgc gccgccgatc cggctgacgc tcagccatcg caatacgacg
      541 gcgctctttg gcgcggggct catcgaccag gttcctgatt cggtgttgca cgctcaggcc
      601 gcagcccaga cgaagcaggg cgaagtctcc ggccgcgtgc cgccggtcgg catcgacttc
      661 gtcggccgct tcggctggcg cggacagacg gagcgcctgc acgatttcgt cctcggagcg
      721 tgcgccaacg agctgggatt ggaagtgccg ggcatcccgc agccgcggaa tccgctgcag
      781 ccggactacc gcccggcggg cctcgacctg accgcggccc aatgtgcatc gctcacctcg
      841 ttcgtggctt cgctgccgcc gccgaagctc ttgccgccgg acgaaccggc caagcgagcc
      901 gtcttcgacc gcggacaggt tctgtttcat tccgtcggct gcgcgagctg ccacgtcgag
      961 aagatcgggc cgctcgacca tgtctatagc gacctgttgc tgcacgatat gggaccggct
     1021 ttggccgatc cgctcctggc ggctcccgca cttcgcgtca tcaagcaaac gccgctcgaa
     1081 cagagcgatg ccgaactgct gctggggagc acgcagcccg tctcgtcgca gccaaaactc
     1141 ccgcagccgc agcttccgcc gccgcgcggg tactacggcg aatccagctc ctcgagcttc
     1201 gcgctcctcg gcgaggaagg cccggcgacc atcacggttg tcgatcgcaa ggcaggggta
     1261 aaggaggagc tgcgagccgt cgtttcgccg ctggaccgtg agtggcggac gccgccgctc
     1321 tggggcctgg ccgattcggc tccgtacctg cacgacggcc gggcggcgac cgtggtggaa
     1381 gccatcgcct tccacgccgg cgaagccgac gcctgcacga agcgctactt cgccctcccc
     1441 gccggcgacc ggatggccgt gctccagttc ctggcctgcc tccaggcgcc gaaatagggt
     1501 tgccccgccg gcgcgcaaac acgtcgttcc caggttccag cctgggacgc ccttctcccc
     1561 gaggctccca cctcgcgtat cgttggcttt gctactccgt cccaggctct gcctgggacg
     1621 gaacgtttcc gaggctcctg cctcgcgcga gccggagggc gcagcgcgca actttcgttg
     1681 tttttttcac gcccctcctt cccgcgcaac atcctcgcaa ctccattccc aataaccact
     1741 tgcgccgcgc aacacccccc gcatgaggat gttgcgctgt tgcgcttgga caaccagtac
     1801 ctcgttccca ggctccagcc tgggaacgcc ttctcctgga ggctcctgcc tcgggaggcc
     1861 cagcgcaact ttgtttcctc cgtcccaggc tctgcctgcg acggaacgtt tccgaggttc
     1921 ctgcttcgcc cgagccgggg gggcgctgcg cgcaactttc gctgtttttt tcacgccctt
     1981 ccttcccgcg caacattatt gcaactccat ttccaacaac cacttgcgct gcgcaacacc
     2041 ccccagatga ggatgttgcg ctgttgcgcg ccatcaagat gttgcgcaaa cccctcgttt
     2101 ccgcctggga acgcacgtcc aggagtctcc tgccttccga aaccggaact acgcgtcggc
     2161 ccaaaatggc ccgccagcgc tcccccgata atgataacaa gtacactatt gttcggaagt
     2221 ttacaaaacg cttccctgcg ttccaagcga attccgccga ttttctccac ccgcgccgac
     2281 ttacggcgac gcgccagaac ataaactcct gtccaccaat accttgcgtc ctcgccccgc
     2341 gtcctaactt tttttcaccc gccccaccga aagggacacg tctggtccag ccgcatccct
     2401 gacacgcgcg ccgctaccgc tctagcgatg ccttgacctg gggcggcgct tgcggatcgg
     2461 gcttcaggcc aacgagcggc tggacgttct cgcggaccca gatgttgcgg aaccgcaccg
     2521 ggttgccgtg aaactgaatc gacagcggct ccttgtcggc gtgggccgtg tatttcggcg
     2581 gctcggtgta cgacgtgctc ccctgcagct cgaagtgatt ctggatcacc agcccgttgt
     2641 gcagcacggt cacgtagccg ggcgatttca gcgtgccgtc gtcgtggaac cgcggggcgg
     2701 tgaacaggat gtcgagcgat tgccactcgc ctggccggcg gctggcgttc accgttggtg
     2761 gctgctgctt gtagatcgct ccgcactggc cgtcgaagta ggtctggttg tcgaacgaat
     2821 cgagaatttg cacctcatag cggcccatca agtacagccc gctgttgccg cggccttggc
     2881 cgcttccctt cacttcggcc ggcgtggcga attccagatg cacctggcag tcgccgaact
     2941 tctgctgcga cgtgatgctg ccgccggcag ccgtggccac gccatcggcg atctgccact
     3001 tgtcgcccgc ttcccaggcg tccaggttct cgccaccgaa gaggaccacc gcatcagccg
     3061 gcggctggcc cgcctcgccc ggatcgacca cggggggctc gggccagacg atgccgctct
     3121 tatattcatc gacccacatg cccaccgccg gcaccgccag cgcgagggcg gccagcaaga
     3181 caacccggtt cgttcgcatc gcacgtgctc cagaaatgac aatgaaagcc ggcagccaat
     3241 ttgccacgcg ccgccgcaat cacgagtata gctgctgccg cgtttgcctt gccgtgccag
     3301 cgcccgtctg tcacactgga ggccatccgg cccgcttgcg atccagtcgt cgaatcccgc
     3361 catgaagcct tcccgcctgc tcgcacttct tctgttttcc gtgtcttccg tgtgctgcgt
     3421 ggttcctttg acggccgcgg aactcgccac gcagcttcgc cggccgatcg ccctcgtcca
     3481 atcggccgat ggcaggcagc ttttcgtggc caatcgcgat agcggcagcg tatcggtgat
     3541 cgatattggc gagcggcagg ttgtggcgga gcaggcaatc ggcaagcggc tggcggacct
     3601 ggccgcgctg cccggctccg accggctgct ggccatcgat gaggcggctc acgagctgct
     3661 gctgcttcag ccgggcgcgg aaggcctgga aatcatccag cgcctgccgg tcagtccgta
     3721 ccccgtgacc atctgcgtgt tgccaggcgg caagcaggcc acgattgcct cgctctggtc
     3781 gcagcggctg acgttcgttg aattgggtaa gactctcaag atcgattcgg ttctcgatct
     3841 gccatttgcg ccccgttgtc aattggttct gcaaaacgac ggcagacgga gtatgcctgc
     3901 taccttgctc gtcgccgatt ccttcagcgg caggttggcc ctcatcgacc ccgccaatcg
     3961 caagctgacg gccacgcgcg aattccccgg gcataacgtg cgcggcctcg gcgcgagccc
     4021 cgacgggcgg atgctgattg tcgcccacca gatgctcaac gagctggccc actcgatccg
     4081 caac
//
LOCUS       DASUGB010000531         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1171494, whole genome shotgun sequence.
ACCESSION   DASUGB010000531 DASUGB010000000
VERSION     DASUGB010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171494"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VFV87_10250"
     CDS             <1..1277
                     /locus_tag="VFV87_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5104183.1"
                     /translation="DSLAAFAAGKSQIVFGLRHSPPGGNKMLAGRIDRAALYDRALSA
                     DEVAASAGTVRTSVTSTEIDAALNEATRARRQHLTKEVARLAAEIATYRDRKVFAVTP
                     QEAPLAHVLIRGNPQQPGDVVSPAGIAVLPGADLGLPPDASDADRRRKLAEWIASEKN
                     PLFARTIVNRVWHYHFGRGLVETTSDLGFNGGQPSHRELLDWLACELIDRDWSLKELH
                     KLIVTSATYQQSSRPRAECLAVDADDRLLWRYSPRRLEAEEVRDAMLAAAGRLNSAVG
                     GPSFMDFRPYEYKSTQYYEPIDPVGPEFNRRSIYRMWARGGKNPLLDTFDCPDPSTTT
                     PSRGSTTTPLQALSLLNNSFTLRMADLLAERLEQDRPGEAEEQVARAFELTCGRPASA
                     DEQTASTAFVRQHGLAAFCRVLLNTNAFLYVN"
     gene            1279..2769
                     /locus_tag="VFV87_10255"
     CDS             1279..2769
                     /locus_tag="VFV87_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104184.1"
                     /translation="MPNPQSAFRVPQWSRRQLFSWSAFGLGATALAGMLSRDGVVRAD
                     GIPGEAQDPPPHLPAKAQRVIHICCCGGYSQIDTFDYKPELARRHGQPLGGDEKPDVF
                     FGQVGLLRANDFAFQQRGESGLWVSDLFPHLAEVADELTVIRSMFAESSSHTPATMHE
                     NSGFRLNGFPALGTWLSYGLGNETDELPAYVVLPDPRGLPAGGTNNWTSGFLPAQHQG
                     VMFNTTRGAGVSPARDSQVIRDLNPARPIAANIESASRELHRTLNELHQAEVGESSEL
                     TARMKAYELAAKMQLAVPRVTDLSGETAATLALYGLDQEKTAPFGQNCLLARRLIEQG
                     VRFVQLFSGGAFGSPRINWDGHEDVKALHTQEAGLIDQPVAGLLKDLRQRGLLEDTLV
                     LFTTEFGRTPFTQSAADQVGAGRDHNQYGFSVWLAGAGLKHGLAYGATDDVGWKAIEN
                     RVAWHDFHATVLHLLGIDHERLTFYHNGIQRRLTNVHGNVIRGILA"
     gene            complement(2770..>3317)
                     /locus_tag="VFV87_10260"
     CDS             complement(2770..>3317)
                     /locus_tag="VFV87_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104185.1"
                     /translation="AFAKGQADKPEKQDAASARDDAAGSRPAVQSRPSTPAAPASEPA
                     ASAPPAPAPAAQSPTSPLADSESDQNEKPAEAIAGKGNARGVPLNQPQSAARPTAKDS
                     LGDGQETQEKLAELKAKGAVAAAPPSIYFNPKLIADENGRATIEFTLPPVESEYRLLI
                     DALGNGRIGSLQQVIEVKSAK"
BASE COUNT          552 a         1128 c         1065 g          572 t
ORIGIN      
        1 cggactcgct ggccgcgttc gccgccggca aaagccagat cgtcttcggc ctgcgacaca
       61 gcccacctgg cgggaacaag atgctcgccg gtcggatcga tcgggcggcg ctttacgacc
      121 gggcgctgtc cgcggatgaa gtcgccgcct ctgcggggac ggtccggaca agcgtcacca
      181 gcacggagat cgacgcggcg ttgaatgagg cgacacgcgc ccgccgccag cacctgacca
      241 aggaggtcgc tcgcctggcc gcggaaatcg cgacgtatcg ggaccgcaag gtctttgccg
      301 tcacgcctca ggaagccccg cttgcccatg tcctgatccg tggcaacccg cagcagccgg
      361 gcgacgtggt ttcgccagcg ggaattgccg tgttgccggg ggcggacctg ggcctgccgc
      421 ccgatgccag cgacgccgac cgccgccgga aactcgccga gtggatcgcg agcgagaaga
      481 acccgctctt cgcccggacg atcgtcaacc gcgtctggca ctatcacttc ggccgcggac
      541 tggtcgagac taccagcgac ctcggcttca acggcggcca gccatcgcat cgcgagttgc
      601 tcgattggct ggcgtgcgag ctgatcgacc gggactggag cctcaaggag ctgcacaagc
      661 tgatcgtcac atcggcaacc taccagcaat cctcccgccc gcgagccgag tgcctcgctg
      721 tcgatgccga tgatcgcctg ctgtggcgct atagcccgcg gcggttggag gcggaggaag
      781 tccgcgacgc catgctggcc gcagccgggc gattgaattc ggctgtcggc gggccgagct
      841 tcatggattt ccgtccgtac gaatacaaat cgacccagta ttacgaaccg atcgatccgg
      901 tcgggcccga gttcaaccgc cgcagcatct atcgcatgtg ggcccgcggc ggcaagaatc
      961 cgctgctcga cacattcgac tgccccgatc cttcgaccac cacgcccagt cgcggcagca
     1021 ccaccacgcc gctgcaagcc ctctcgttgc tcaataattc gttcacgctg cggatggccg
     1081 acttgctggc ggagcgcttg gagcaggacc gtccgggcga ggccgaagag caagtcgctc
     1141 gcgcgttcga gctcacctgc ggccgtccgg cttcggcgga cgaacaaacg gcctccacag
     1201 cgtttgtcag gcaacacggc ctcgccgcct tctgccgagt tctcctgaac acgaacgcgt
     1261 tcctttacgt gaactgaaat gcccaatccg caatccgcat tccgcgttcc gcaatggagc
     1321 cgccgccagc tcttcagttg gtctgcgttc ggcctgggtg cgacggcgct ggcgggaatg
     1381 ctgtcacgcg acggcgtcgt gcgcgccgac ggaatccccg gcgaggccca ggatccgccg
     1441 ccgcacttgc cggcgaaggc ccaacgcgtg atccatattt gctgctgcgg cggatacagc
     1501 cagattgaca cgttcgacta taagcccgag ctggcccgcc gccacggcca gccgctgggg
     1561 ggcgacgaaa agccggatgt cttcttcgga caggttggtc tgctccgggc caacgacttc
     1621 gccttccagc agcggggcga gagcggcctg tgggtcagcg acctctttcc gcacctggcg
     1681 gaagtggccg acgagctgac cgtcatccgc tcgatgtttg ccgaatcgtc cagccatacg
     1741 ccggccacga tgcacgaaaa ctccggcttt cggctgaatg gctttccggc tctggggacg
     1801 tggctgtcgt atggactggg gaacgaaacc gacgaattgc cggcctatgt cgttctgccc
     1861 gatccccgcg gcctgcccgc cggcggcacg aacaactgga ccagcggctt cctccccgct
     1921 cagcaccagg gcgtgatgtt caacacgacc cgtggggcgg gcgtctcgcc tgcccgtgat
     1981 tcgcaggtca ttcgcgacct gaaccccgcc cgtcccattg ccgcgaatat cgaatccgcc
     2041 agccgcgaac tccaccgcac gctcaacgag ctgcaccagg ccgaagtcgg cgaaagcagt
     2101 gaattgacgg cccggatgaa ggcttacgaa ctggccgcca aaatgcagct cgccgtccca
     2161 cgggtgacgg acctctccgg cgaaacagcc gcgacgctcg ctctctacgg cctcgaccag
     2221 gagaaaacgg ctcccttcgg acaaaattgc ctgctggccc ggcggctgat cgagcagggc
     2281 gtccgcttcg tgcagctttt ttccggcggc gcgttcggca gcccgcggat caactgggac
     2341 ggccacgagg acgtcaaagc cctgcacacg caggaagccg gcctgatcga ccagccggtc
     2401 gcgggcctgt tgaaagacct ccggcagcgc ggcctattgg aagacacgct cgtcctgttc
     2461 acgaccgaat tcggccgcac gcccttcacg cagtccgccg ccgaccaggt cggcgccggc
     2521 cgagaccaca accagtacgg cttctccgtc tggctggccg gcgcgggact gaagcatggt
     2581 ctcgcctatg gtgcgaccga cgacgtcggc tggaaagcca ttgaaaaccg tgtcgcctgg
     2641 cacgatttcc acgccaccgt cctgcacctc ctgggcatcg atcacgagcg gctcacgttc
     2701 tatcacaacg gcatccagcg ccggctgacg aacgtgcatg ggaatgtcat ccgcgggatt
     2761 ctggcgtagc tatttcgcgc tcttgacttc gatcacttgc tgcagcgagc cgatgcggcc
     2821 gttgccgagg gcgtcgatga ggaggcgata ttccgattcg accggcggga gagtgaactc
     2881 gatcgtggcc cggccgtttt cgtcggcgat cagcttggga ttgaagtaaa tcgacggcgg
     2941 agcggcggcg acagcacctt tcgctttgag ctcggccagc ttctcctggg tttcctggcc
     3001 gtcgccaagg ctatctttcg cggtgggcct ggctgccgac tggggctggt tgagcggcac
     3061 gccccgcgcg ttgcctttcc cggcgatcgc ttcggcaggc ttttcattct ggtccgattc
     3121 ggagtcggcc aacggcgagg tcggcgattg agcggctggc gcgggagcgg gcggcgctga
     3181 agcggcaggc tcgctcgctg gcgcggccgg agtgctgggc ctggactgca cggcaggccg
     3241 gctgccggct gcatcgtctc gggccgacgc ggcgtcctgt ttctcgggct tgtcggcctg
     3301 gcccttcgcg aatgccc
//
LOCUS       DASUGB010000532         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_59651,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000532 DASUGB010000000
VERSION     DASUGB010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59651"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            570..2090
                     /locus_tag="VFV87_10265"
     CDS             570..2090
                     /locus_tag="VFV87_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5104186.1"
                     /translation="MPTRAPSAAAPSEIRPVAFESQVTIAQPPQPPPDVPADDRLPVP
                     RTPQPQSFREEVAAPQVPPVLGAPPALPLDLPTALGLAQGQNPRIAFAQAQIAQAYAQ
                     NRAARVMWLPSLRAGMNYNKHEGRIQDVIGTNIETSRGAMYGGLGANAVGAGSPAIPG
                     LYMQFHTTDAIYQPRITGYALNARQQQGTAVANDQLLETALAYVDLLEALQREAIATQ
                     SLKHGEALVDLTANFAKTGAGNQADADRASAAAALLRNEAVRAEEAIAVASARLAQQL
                     SADPSIPIAPQESTVVPIDLVPVEMFAGDLVATGLSNRPELAASRALVGEAVSRLQRE
                     QHAPWLPSVVLGLSYGAFGAGRGGDIARTGDRLDLDAIAWWELRNLGFGEAAARANAS
                     AQIQQARMREVETLDLVAREVVEAHAQVVARRKLIGVAEGGIQSAQQSYDRNLARIRN
                     AQGLPIEVLQSIQALDAAQREYLRAVIDYNQAQFRLQRALGWPVTVPISPAGASPG"
BASE COUNT          365 a          706 c          732 g          334 t
ORIGIN      
        1 cccgacagcc gcggatcgaa gaagaggagc ttgccgagcg cgattttctc ctcgctcgac
       61 ggattgtcca tgggagttgg aacggtgtcg agtacggtcg cgagcggtgc atcttttccg
      121 tgctggccaa gcgcgattcc agcaaggagc gccacgagca agcccgccgg gcaaccacag
      181 cgcagtgctc gatggaggaa cgagccagac gatggggcgt ggagcatcgt acattcgcta
      241 caggcgggaa atccgtttcg attctaccct gcgattcaag ccctacaata tcgaagttcg
      301 atattatcag agcaatctgt caacggctgt gctcctccgc gcccgcaggc gtgccgcaag
      361 ttacgcaggc ggaacagacc gcaaagttgc tagacgcttc aacgatgaac gaatttcgca
      421 cttaccatga tctcctgtgg tcgagtctgg cggcggcagc cttggcgctg gcgggctgcg
      481 catcgggtcg gggagttgcg gcgcctgctg cggtcgccga tgtgccgcaa acgccctcga
      541 cgacgggcac gctagagaga ctgcctcccg tgccaacacg tgcgccctcc gccgcggcgc
      601 catcggagat tcggcccgtc gccttcgaaa gccaggtgac gatcgcgcag ccgccgcagc
      661 cgccgccgga cgtgcccgcc gacgatcggc tgccggttcc gcgcacgccg cagccgcaat
      721 cgtttcgcga ggaagtcgct gcgccgcagg tgccgccggt cctcggggcg ccgccggcgc
      781 tgccgctcga tctgccgacg gcactggggc tcgcgcaggg ccagaatccg cggatcgcgt
      841 tcgcgcaggc gcagatcgcg caggcctatg cccagaaccg ggcggcccgc gtgatgtggc
      901 tgccgagcct gcgggccggc atgaactaca acaagcacga agggcggatt caggatgtca
      961 tcggcaccaa catcgagacc agccgcggcg cgatgtacgg cggcctcggc gccaacgcgg
     1021 tcggggccgg ctcgccggcg attcccggcc tgtacatgca attccacacg accgacgcga
     1081 tttatcagcc gcggatcacg ggctatgcgt tgaacgcccg gcagcaacag ggcacggctg
     1141 tcgcgaacga ccagcttctg gagacggcgc tggcttatgt ggatttgctc gaagcgctgc
     1201 agcgcgaggc gattgcaacc cagtccctga agcacggcga ggcgctggtt gatctaacgg
     1261 ccaacttcgc aaagaccggc gcaggcaatc aggccgatgc cgaccgggct tctgcagccg
     1321 cggcgttgtt gcggaacgaa gcggtgcgag cggaggaagc gattgcggtt gcttcggccc
     1381 gtctggcgca gcagctcagc gccgacccgt cgattccgat cgcgccgcaa gagtccacgg
     1441 tcgtgccgat cgacctcgtg ccggtggaga tgttcgcggg agacctcgtt gccaccggcc
     1501 tctcgaatcg ccccgaactc gccgccagcc gggcgctcgt cggcgaagcc gtcagccgac
     1561 tgcagcgtga acagcacgct ccgtggctgc cgagcgtggt gctggggctg agctacggcg
     1621 cattcggcgc ggggcgcggc ggcgacatcg cccggacggg cgaccggctc gacctggatg
     1681 cgatcgcctg gtgggaactg cggaatctcg gctttggcga ggctgcggcg cgagccaatg
     1741 ccagcgcgca gatccagcag gcgcggatgc gggaggtgga gactctggac cttgtggccc
     1801 gtgaggttgt ggaagcgcat gcgcaagtcg tcgcccgccg caagctgatc ggcgtggcgg
     1861 aagggggaat tcaatcggcc cagcaatcgt acgatcggaa cctcgcgcgg attcgcaatg
     1921 cccaggggct gccgatcgag gtcctgcagt cgatccaggc gctcgacgcc gcccagcgcg
     1981 agtacctccg tgccgtcatc gactacaacc aggcccagtt ccgcctccag cgggcgctcg
     2041 gctggccggt taccgttcca atcagccccg cgggtgcgtc gccgggctag ccgatagcgc
     2101 cccacgacgc gcggccttcc agcgctcggt cgagatt
//
LOCUS       DASUGB010000533         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_200010, whole genome shotgun sequence.
ACCESSION   DASUGB010000533 DASUGB010000000
VERSION     DASUGB010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200010"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFV87_10270"
     CDS             <1..676
                     /locus_tag="VFV87_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5104187.1"
                     /translation="DLNRRVSQLDLNNADLHRQLAQSEQQTTKYKQQADLLHKEYSAL
                     AAKLNETQLARTEADKKVTALEASTRMRGGATITANSSVKQSLSMIAIPGLEIGQDGE
                     VIRIEIPADKLFTPGSGQLLIEGYRILDEVAAAIQRSYPRQRIVIEGHTDDSLGTNST
                     AAHALSSSQAGAVFQQLVQRGRLPTRQLSVLALGDNHPLASNGTAAGKAKNRRVEVVI
                     YPDSID"
     gene            complement(763..1659)
                     /locus_tag="VFV87_10275"
     CDS             complement(763..1659)
                     /locus_tag="VFV87_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5104188.1"
                     /translation="MRPCLFSVSYAGFWGQAALPLAEFIARAGKLGFVSVMIAGKRPH
                     LSPLDAGPRQLEEVQAALAAAGVTCDVIAGYTNLAPGSASEVPDSELQIAYVESLARI
                     AARLGARAVRIFTAYELDGSDPAAVWRRAVAIIREMCDRAAVHGVAIAVQNHHDLAIE
                     TDALLELVAEIGRTNCKLGFDAWSLALRGEDLYSAAKKAAPHTIITTNADYVRVPRYR
                     YRPELVNYERLAPDLVRAVPFGCGIVDYEAFFHGLRDGGFNGIATYEICSPIRGGGSL
                     ENLDACGGQYLEWMRQRGLLDS"
     gene            1757..>2808
                     /locus_tag="VFV87_10280"
     CDS             1757..>2808
                     /locus_tag="VFV87_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5104189.1"
                     /translation="MVRLTWCILFTFVWMWLVERTPAADQRPNILLIVSDDQGWPDLG
                     CIGTKSIQTPNLDKLAKEGLRLTNFYVTWPACTPSRGSILTGRHPLRNGLYDMVRNDL
                     VNYGHLYTEQEYSTSPEMTLGLDPREQTLGDMLHAAGYRCGMVGKWDMGQAKRYLPLQ
                     RGFDFFYGHGNNGIDYYTHERYGIHSMFRGNERTKEDRGTYSTDLFEREALRFLRGDT
                     NPKRERGIGGDRPWFLYLCFNAPHGASSFGDDPNNPKSRTGVQAPENYVAKYPDANIP
                     DNLKRYFAAVTCMDDAIGQIVTLLRETGQLDNTLIVFQSDNGGSGNGGNAPLKGSKGT
                     LWEGGLRVPCIARWPG"
BASE COUNT          579 a          921 c          840 g          468 t
ORIGIN      
        1 agacctcaat cggcgggtga gccagctcga tttgaacaat gccgacctgc accgccagct
       61 cgcccagtcc gagcagcaga cgacgaagta caagcagcaa gccgatctgc tccacaaaga
      121 gtacagcgcg ctggcagcca agctcaatga aacacagctc gcccgcactg aagcggacaa
      181 gaaggtgacg gccctcgagg cttcaacccg gatgcgcggc ggcgccacga tcaccgccaa
      241 cagcagcgtc aagcagtcgc tgtcgatgat cgcgattccc ggcctggaaa taggccagga
      301 cggcgaggtc atccgcatcg agattccagc cgacaagctc ttcacgcccg gcagcgggca
      361 acttctgatc gagggttatc ggatcctgga cgaagtcgcc gccgcaatcc agcggagcta
      421 cccgcggcag cgaattgtca tcgaaggcca caccgacgac tcgctgggga cgaattccac
      481 ggccgcgcac gccctgtcca gttcgcaagc cggcgcggtt ttccagcagc tcgtgcagcg
      541 cggccgcctg ccgacgcgcc aactttcggt cctcgccctg ggcgacaatc acccgctggc
      601 cagcaacggc accgccgccg gcaaagcaaa gaaccggcgt gtcgaagtcg tcatctaccc
      661 cgactcgatc gattgacatc gccgcgcgtc tcgccccata ggtcggccct ccaggtccgt
      721 cggaaatcgc gctgcttgcg tggacggacc aggaggtcca tcttacgagt cgagcagccc
      781 ccgctggcgc atccactcca ggtactgccc cccgcacgcg tcgagattct ccagcgagcc
      841 gccgccgcgg atcggcgagc aaatctcgta ggtggcgatg ccattgaatc cgccgtcgcg
      901 gaggccatgg aaaaaagcct cgtaatcgac aatgccgcag ccgaacggaa ccgcccggac
      961 caggtcgggc gcgagacgct cgtaattcac cagttccggc cggtagcgat agcggggcac
     1021 gcggacgtaa tcggcattgg tcgtgatgat ggtgtgcggc gcagctttct tcgcagccga
     1081 atacaagtct tcgccgcgga gcgccagcga ccaggcatcg aacccgagct tacaattggt
     1141 ccggccgatt tcggcgacga gttccaagag cgcgtccgtc tcaatcgcca agtcgtggtg
     1201 gttctgcaca gcgattgcca cgccgtggac cgcggcccgg tcgcacattt cgcggataat
     1261 cgccacggcc ctccgccaca ccgcggccgg atcgctgccg tccagttcgt acgccgtgaa
     1321 gatacgaacc gccctcgcgc ccagtctggc agcgatccgc gcgagcgact ccacataagc
     1381 aatctgcaat tctgagtcgg gaacctcgga cgccgagccc ggagccaggt tggtatagcc
     1441 cgcgatcacg tcgcacgtta ctcccgccgc agccagggcc gcttgcacct cctccaattg
     1501 ccgcggcccg gcatccagag gcgacaggtg tggccgcttc cccgcgatca tcactgacac
     1561 aaaccccagc ttcccagcgc gggcaatgaa ctccgccagc ggcagcgcag cctgtcccca
     1621 gaagccggcg taactgacag agaacagaca cggtcgcatg gcgggctatc gatggaggga
     1681 gatgctatcg acgtgtggac tggttactat gatttgcgag tcgtggtcgt ttcgcaagtg
     1741 aattcgggag gcggacgtgg tgcggttgac ctggtgcatc ctgtttacat tcgtctggat
     1801 gtggcttgtg gagcgaactc cagcagctga ccagcgtccc aacatcctcc ttatcgtcag
     1861 cgatgatcag ggctggcccg atctcggctg catcggcacc aagtccatcc agacgccaaa
     1921 tctcgacaag ctggcgaagg aagggttgcg gctgacgaat ttttatgtga catggccggc
     1981 gtgtacgcct tcgcgcggca gcattctcac ggggcggcat ccgctgcgaa acggcctgta
     2041 cgacatggtc cgcaacgacc tggtcaatta cggtcatctc tacaccgagc aggagtattc
     2101 gacgtcaccc gaaatgacat tgggcctgga tccacgcgag cagacgctcg gcgacatgct
     2161 ccacgccgcc ggctaccgct gcggaatggt cggcaagtgg gacatgggcc aggccaagcg
     2221 atatctgccc ttacaacgcg gtttcgattt cttctacggc cacggcaaca acggcatcga
     2281 ctactacacg cacgagcggt acggcattca ctcgatgttc cgcggtaatg agcggacgaa
     2341 agaggatcgg gggacatact cgaccgatct cttcgagcgg gaggcgctgc ggttcctgcg
     2401 cggcgacacg aacccgaagc gtgagcgagg aatcggcgga gatcggccgt ggtttctcta
     2461 tctctgcttc aacgcgcccc atggagcatc cagcttcggc gacgatccga ataatccgaa
     2521 atcgcggacg ggcgtgcaag cgcctgagaa ttacgtcgcg aagtatcccg acgccaacat
     2581 tcccgacaat ctgaaacgct actttgccgc cgtcacttgc atggatgacg ccatcggcca
     2641 gatcgtcacg ctcctgcggg aaacggggca actcgacaac acgctcatcg ttttccaatc
     2701 cgacaacggc ggcagcggca atggcggcaa tgcgccgctc aaagggagca agggcacact
     2761 ctgggaaggg ggcctgcgcg ttccctgcat cgcccgctgg cccggcaa
//
LOCUS       DASUGB010000534         5470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1171878, whole genome shotgun sequence.
ACCESSION   DASUGB010000534 DASUGB010000000
VERSION     DASUGB010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171878"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..287
                     /locus_tag="VFV87_10285"
     tRNA            214..287
                     /locus_tag="VFV87_10285"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Val,seq:cac)
     gene            753..2081
                     /locus_tag="VFV87_10290"
     CDS             753..2081
                     /locus_tag="VFV87_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104190.1"
                     /translation="MKRREFLRQSAAAVGVLACPAILHSAAPNSLLQVACIGVGGMGG
                     NTMKSVASHSKVKIVALCDVDSRALDAAGKEHPQASRHNDWRELLAEHAGRFDAVTIG
                     TPDHMHAAQAVTALRAKKHVYLQKPMAATLHECRVITEEAAKAGVVTQLGNQGRSSTE
                     SRMTVELLRSGAIGKIKEVLLWENKPLNWWPKNTQLRPDPDAIPAGLDWDLWLGVREP
                     RPYLENTYHPKNWRAWFDFGVGEMGDMGCHHFDTSFDALQLTAPLRVRQTTPGSSGAL
                     WGKKRVVEIIFPGTPYTAAETVQLTWHDGGIEPDRKKIQLPQEIEQFPLSATYWIGER
                     GAIFKVYGRGKPIVLPEAEFPLEKYPAGLQPQDHYHDWVDAILHSRKACDEFSHGGPL
                     TEAVLVGAMADRVAGEWLEWDAKALKFSDPRASELVRREYRDGWQVDGLG"
     gene            2193..3590
                     /locus_tag="VFV87_10295"
     CDS             2193..3590
                     /locus_tag="VFV87_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5104191.1"
                     /translation="MAAQPARPNIVLVFIDDMGWGDFSCFGNPDAKTPNADRLAAEGI
                     RFSQFYVNAPICSPSRCALTTGQYPQRWKITSYLNNRADNARRGVANWLDPEAPTLAR
                     ILKAHGYATGHFGKWHLGGQRDVDDAPPISAYGFDQSLTNFEGMGAKLLPLTLRPGEE
                     QPGRIWADAERLGGPVTWMQRSQITAGYVDAAIAFIDRATREQQPFYVNVWPDDVHSP
                     FWPPVDKWAGGKKGLYLSVLQEMDRQLGKLLDHIRESPALRDSTLILVCSDNGPEPGA
                     GSAGPFRGAKTRLYEAGIRSPLIVWGPSLIPREKQGAHNETSVFAAFDLVPSLVTLTG
                     AAAPAEANFDGEDVSETLVGKSAASRRAPIFWRRPPDRKNHPPALPDPQPDLAVRDGD
                     WKLLCNYDGSQPELYDLARDRGETTNLAAKHPDIADRLTAAALEWHRSLPADNGPKLG
                     PTSPDSAPRQKQKPR"
     gene            3641..5200
                     /locus_tag="VFV87_10300"
     CDS             3641..5200
                     /locus_tag="VFV87_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5104192.1"
                     /translation="MKAATFTILLVLLSSQAQADAPRPNILFAIADDWGAHAGAYGTD
                     WVKTPSFDRIAREGLLFTNAYTPVAKCAPSRAIVLTGRHAWQNEEAGKHMAYFPARLK
                     GWPEVLAEHGWHMGFTGKGWGPGIANDASGKPRQLTGQPFNQRKAKPPTASIGNNDYA
                     ANFRDFLDAAPKDAPWCFWYGSTEPHRGYEFQSGANKGGKKLADIAKVPNYWPDDDIV
                     RHDILDYAFEVEHVDNHFGRMIAELENRGLLDRTLIIVTSDHGMPFPRVKGYAYRDSN
                     HVPLAIRWPGGVQRPGRAIEDFVDFTDIAATLLDYAGIEAKDSGMLPLTGKSWRPILE
                     SDKSGQIVPQRDHVLVGKERTDVGRPHDWGYPIRGIVTKTHLYLRNYEPTRWPAGNPE
                     TGYLDTDGSPTKTLILEMGRKNRADRYWQLNFGMRPADELYDLVSDAACANNLAADSS
                     YSERLAQLRERMEAELKTQEDPRMSGRGRVFDEYPPTSGVGFYEKFIRGEKPNAGWVS
                     PTDFEKEPIQP"
     gene            5197..>5470
                     /locus_tag="VFV87_10305"
     CDS             5197..>5470
                     /locus_tag="VFV87_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5104193.1"
                     /translation="MRLLYLFLALLLAIPFAIAAPQPNFVLMMGDDHGWEETGYNGHP
                     HVKTPVLDELAATGLRLERFYAAHPSCSPTRASFLTGRHPNRMGTFA"
BASE COUNT         1048 a         1760 c         1692 g          970 t
ORIGIN      
        1 cccagccaaa agccgcatgc cctagtgtga ttcggagggt gaaaagattc aatggtttgt
       61 ttggccatca cgatgacgcg tcaccgcgag ctctgtgctc gagggcttcc cgcagtcatt
      121 ccatcggccc aggctgcccg ctgccatcct ctcgacgagc cagtcggccc cgcgttgcct
      181 tccctcgcag ctagggtaaa ctggctttgt tcggggcggc tagctcagtt ggttagagcg
      241 ccatgctcac accgtggagg tcacaggttc gagccctgtg ccgcccattg aagcctaagt
      301 ctcgctgaaa tcagcagttc gattacctgc cagcggttgg ccgtgcagag tttcccggca
      361 gccaaagcgg tatatgccgt tttgagtcag ttttttggaa gacgtggcat aatgcacgtc
      421 tcgttcggca acacattggc tttctcgttc gttctctggc aagcgtggaa ctggagcgct
      481 accgataagc gcccaaatgg cgagcagttt gtcctcccgc tgccgggaga ctgcattagc
      541 cctcccaatg ccatggccct aacggaaacg ttcacctgat caatggatca atggcccaga
      601 cggattgtgg gtcacagatg gtgaaatgat ggccgagcgc ggttacaaag gcgttctgct
      661 gcgggcacgc tgtgccgggc aaaacccaat cggctggcat cgcctaactt cgccgccagc
      721 caaattctca tttttcgacg ggaggccgtg ccgtgaagcg ccgagaattc cttcgacaat
      781 ccgccgctgc tgtcggtgtg ctggcgtgtc cagcgattct gcacagcgcc gcaccgaact
      841 cgctgctgca ggtggcgtgc attggcgtcg gaggcatggg cggcaatacg atgaagagcg
      901 tggccagcca ctccaaggtg aaaatcgtcg cgctctgcga cgtggatagc cgcgccttgg
      961 atgccgcggg caaggaacac ccgcaggcgt cgcggcacaa cgactggcgc gagttgttgg
     1021 ccgagcacgc aggcagattc gacgccgtga cgatcggcac gccggaccac atgcatgcgg
     1081 cacaggccgt taccgcgctg cgggccaaga agcacgtcta tctgcaaaag ccgatggcgg
     1141 cgacgctgca cgagtgccgc gtcatcaccg aggaagccgc caaggcgggc gttgtcacgc
     1201 aactcggcaa ccagggccgc tccagcactg aaagccgcat gactgtggag ctgctccgga
     1261 gcggggcgat cggcaagatc aaagaagttc tcctctggga gaacaagccg ctgaattggt
     1321 ggccgaagaa tacccagctg cggcccgacc cagacgccat tcccgcggga ttggattggg
     1381 acctgtggct gggcgtccgt gagccgcggc cctatttgga gaacacctat cacccgaaga
     1441 attggcgggc ctggtttgat ttcggcgtcg gcgaaatggg cgacatgggc tgccaccatt
     1501 tcgacaccag cttcgatgcc ctccaactca cggccccgct ccgcgtgcgt caaacgacgc
     1561 ccggcagcag cggtgccttg tggggcaaaa agcgcgtcgt cgaaatcatc tttcctggga
     1621 cgccgtacac agcggcggag acggtgcaac tcacctggca cgatggcggc atcgagccgg
     1681 accgcaagaa aatccagctc ccccaggaga tcgagcagtt tccgctatcc gccacctatt
     1741 ggatcggcga gcgaggcgcg atcttcaagg tctatgggcg cggcaagccg atcgtcttgc
     1801 cagaagcgga atttccgctc gagaagtatc ctgccggctt gcagccgcaa gaccactatc
     1861 acgattgggt ggatgcgatc ctgcactcgc gcaaggcctg cgatgaattc agccacggcg
     1921 gccccctcac cgaagccgtt ctggtgggcg cgatggctga ccgcgtcgcc ggcgagtggc
     1981 tggagtggga tgccaaggcc ctaaagttca gcgacccgcg ggcgagcgag ctggtgcgcc
     2041 gcgagtatcg cgacggctgg caggtggatg ggcttggctg agaacttgcg cgtgatctga
     2101 ttcgaaacgc agccacaggc gcggctatga agacgacgat tgaactgctg gtgatcgcgg
     2161 gcgcgcttct cgctgcgcca tccgattcgc tgatggcggc tcagccggct cgtcccaata
     2221 ttgtgctggt cttcatcgac gacatgggct ggggcgattt ttcctgcttc gggaaccctg
     2281 acgcgaaaac gccgaatgca gatcgactgg cagccgaggg gatccggttc tcgcagttct
     2341 acgtcaatgc gccgatttgc tctccctcgc gctgtgcgct gacgactggc cagtatccgc
     2401 agcggtggaa gatcacgtcg tacttgaaca accgcgcgga caatgctcgc cgcggcgtgg
     2461 cgaactggct tgatcccgag gcgccgacgc tcgctcgaat cttgaaggcc cacggctacg
     2521 cgaccgggca tttcggcaag tggcatctgg gcgggcagcg cgatgtggac gacgcgccgc
     2581 cgatttcggc ctacggcttc gaccagtcat tgacgaactt cgaaggcatg ggggccaagc
     2641 tgctgccgct gacgttgcgg ccaggcgagg agcagccggg gcgcatctgg gccgatgccg
     2701 agcgtctcgg ggggccggtc acttggatgc agcgttcgca aatcacagcc ggttatgtgg
     2761 acgcagcgat cgcttttatc gaccgagcca ctcgcgagca gcagccgttc tatgtcaatg
     2821 tttggcccga cgacgttcac agcccgttct ggccccccgt ggacaagtgg gccggtggta
     2881 aaaagggcct gtatctatcg gtcctgcagg agatggatcg tcagctcggc aagcttctcg
     2941 atcacattcg cgagtcgccc gcactgcgcg acagcacgct gattctggtt tgctccgaca
     3001 acggccctga gccgggcgcg gggagcgccg ggccgtttcg cggcgccaag acgcgcctgt
     3061 acgaagcggg cattcgctcg ccgctgattg tctgggggcc cagcctgatc ccgcgcgaga
     3121 agcagggcgc ccacaatgaa acctccgtct tcgcagcgtt cgatctcgtt ccttcgctgg
     3181 tcacgctcac cggcgcggcc gcaccagcgg aggccaattt cgacggcgaa gacgtttccg
     3241 agactctggt cggcaagagc gcggcatcgc gacgtgcgcc gatcttctgg cgtcggccgc
     3301 ccgatcgcaa gaaccatccg ccggcgctgc cggatccgca gcccgatctc gccgtgcgcg
     3361 acggcgactg gaagctgctt tgcaactacg atggttcgca gcctgagctg tatgatctgg
     3421 cccgcgaccg cggggaaacg acgaacctgg ctgccaaaca tcccgacatc gccgaccgcc
     3481 tcacggctgc tgccctcgag tggcatcggt cgctgccggc ggataacggc ccgaagctgg
     3541 ggccaacgtc gcctgattcg gctccacgcc agaaacaaaa accgcggtga tagagtggcc
     3601 cagatcgagc accagccgca cagcgcaaag aagctccacg atgaaagccg cgacgtttac
     3661 catcctcctc gtccttctca gttcccaggc gcaggctgac gcgccgcggc cgaacatcct
     3721 gtttgcgatc gccgatgact ggggcgcgca cgcaggcgcc tatggcaccg actgggtgaa
     3781 aacgccctca tttgaccgca tcgcccgcga agggctgctg ttcacaaacg cctacacgcc
     3841 cgtcgccaag tgtgctccct cgcgggccat cgtcctcacg ggccgacacg cctggcagaa
     3901 cgaggaggcc ggcaaacaca tggcctattt ccccgccagg ctgaaaggct ggccggaggt
     3961 ccttgccgag cacggctggc acatgggctt caccggtaag ggctgggggc cgggcatcgc
     4021 caacgacgcc agtggcaagc cgcgccaact taccggccag ccgttcaatc agcgcaaagc
     4081 caagcccccc accgccagca tcggcaacaa cgattacgcg gccaatttcc gcgattttct
     4141 ggatgccgct ccgaaagatg ccccctggtg tttctggtat ggctccacgg agccgcatcg
     4201 cggttatgag tttcagtcgg gcgccaacaa aggcggcaag aagctcgccg acatcgcgaa
     4261 agtgccgaat tactggcccg atgacgacat cgtccgccac gacatcctcg attacgcctt
     4321 cgaggtggag catgtggaca accatttcgg ccgcatgatc gccgagttgg aaaatcgcgg
     4381 cttgctcgat cgcacgctga tcatcgtcac cagcgaccac ggcatgccct ttccgcgcgt
     4441 gaaagggtac gcctaccgcg attcgaacca cgttccgctg gcgattcgct ggcccggcgg
     4501 cgtgcagcgc cccggccgcg ccatcgaaga cttcgtcgat ttcaccgata tcgccgcgac
     4561 actgctcgac tatgccggca ttgaagcgaa ggacagcggc atgctgcccc tgacgggcaa
     4621 gagctggcgt ccgatcctgg aaagcgacaa gtccgggcag atcgtgcccc agcgcgacca
     4681 cgtccttgtc ggcaaggagc gcaccgacgt gggccgcccg cacgactggg gctacccgat
     4741 tcgcggcatc gtgaccaaaa cacatctcta cctgcggaac tatgagccga cacgctggcc
     4801 cgccggcaat ccagaaaccg gctatctcga caccgacggc agcccgacga agaccttgat
     4861 cctggaaatg ggtaggaaga atcgtgcgga tcggtactgg caactcaact tcggcatgcg
     4921 ccccgccgac gagctttacg acctcgtctc cgacgccgcc tgcgcgaata acctggccgc
     4981 cgactcctcg tacagcgagc gcctcgcaca attgcgcgag cgcatggaag ccgaactcaa
     5041 aacccaggaa gacccgcgga tgtctgggcg cgggcgtgtc ttcgatgagt acccgcccac
     5101 cagcggcgtc ggcttctacg agaagttcat acgcggcgag aagccgaacg ctggttgggt
     5161 cagcccgacg gatttcgaga aggagcccat ccagccatga gactgcttta cttgttcctg
     5221 gccttgctgc tcgcaattcc ttttgcgatc gccgcgccgc agcccaactt cgtcctgatg
     5281 atgggcgacg atcacggctg ggaagagacg ggctataacg gacatccgca cgtcaaaact
     5341 ccagtgctcg acgagcttgc cgccaccggc ctgcggctgg agcggtttta tgccgcccac
     5401 ccgagctgct cgccgacccg cgccagtttt ctgaccggac gccatccgaa ccggatgggc
     5461 accttcgcgc
//
LOCUS       DASUGB010000535         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2006111, whole genome shotgun sequence.
ACCESSION   DASUGB010000535 DASUGB010000000
VERSION     DASUGB010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006111"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFV87_10310"
     CDS             <1..1174
                     /locus_tag="VFV87_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104194.1"
                     /translation="PMNITRREFVAAAAAGSAAVVGRSLLASESAPAGAKPAASAITA
                     KSWPLFRGDSLAQGFSPSSLPAKPEVLWKHTVQGGAFDGTPVIDGGIVYVGDMDGKVY
                     ALKLDTGDEVWTYKVESGFIAAPAVRGNLLYIGDIDGKFYALDCKNGQPKWTFQADAE
                     IDSGANFWKGNVLFGSQDAHLYCLNAEGKLVWKFQIQDQIRCMPTIVGDCSFVAGCDS
                     MLHIIDLNEGKETASVPIDAPTGVTPAVLGDNVFFGTEAGVFFAIDWKEARVSWKKED
                     SNGSQSIRSSPAVQPGIVVVGSRNRSVQAFDPATGKELWTFASKQRIDSSPVIVGNRV
                     LVGAADGRLYALDIKTGQPTWEYQATGGFSGSPAIADGKLVIASDRGVVYCFGKKS"
     gene            1185..1751
                     /locus_tag="VFV87_10315"
     CDS             1185..1751
                     /locus_tag="VFV87_10315"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5104195.1"
                     /translation="MASSVATGEKLMTIEQFAAIPHDDRQRELVKGKVVEMNLPKPKH
                     GFICGEIAGLLREFVKPRKLGRVFSNDSGVITERDPDSLRGPDVGFYSFARVPAGPIP
                     DGYYAVAPEIAFDVLSPDDVWARMLEKVAELLDAGVVVVCVVDPRDETVQVHRADQPI
                     QYLQRGDELTVPDVLPGFSCKVSQIFEI"
     gene            1752..>2613
                     /locus_tag="VFV87_10320"
     CDS             1752..>2613
                     /locus_tag="VFV87_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5104196.1"
                     /translation="MTTEATKTEVGSYFIANYPPFSQWSVEQLPELEKALHSPPKAVP
                     LGLYLHIPFCRKRCKFCYFRVYTDKTAPDVERYVQALSHEIGLVSQLPVMGGRPFRFV
                     YFGGGTPSFLSAKQLTSLVDRLRQHVNWDHAEEVTFECEPGTLSEPKVKTLKELGVTR
                     LSLGIENFSDAILEENGRAHLSAEVYKSWDWIQAAGFPNVNIDLISGMVGETWENWRE
                     NIKKTIDLSPDSVTIYQMELPFNTVYSKDILGNHVETPVADWPTKRAWLNYAYDELIS
                     AGYHVSSAYTV"
BASE COUNT          522 a          761 c          812 g          518 t
ORIGIN      
        1 accgatgaac attacgcgac gcgaattcgt ggcggcagcg gcggctggct cggcggcggt
       61 ggtcggtcgg tcgctgttgg cgtccgagag cgcgcccgcg ggcgccaagc cagcggcatc
      121 cgctatcacg gccaagtcgt ggccgctttt ccggggcgat tcactggccc aggggttcag
      181 cccctcgtcg ctgccggcca agcctgaggt gctgtggaag cacaccgtcc aaggtggggc
      241 cttcgacggc acgccggtga tcgacggcgg catcgtctat gtgggcgaca tggatggcaa
      301 agtctatgcc ctgaagctcg acaccggcga tgaggtctgg acctacaaag ttgagagcgg
      361 cttcatcgcg gcgccggcgg ttcgaggaaa cctgctttat attggtgaca tcgatggcaa
      421 gttctatgct ctggattgca agaacggcca gcccaagtgg acatttcaag ccgacgcgga
      481 aatcgactcg ggggcgaact tctggaaagg gaacgtgctt ttcggctccc aggatgcaca
      541 tctgtattgc ttgaacgcag aaggcaagct cgtctggaaa tttcagattc aggaccagat
      601 ccgctgcatg ccgacgatcg tcggcgattg ctcattcgtc gccggctgcg acagcatgtt
      661 gcacattatc gatctcaacg aaggtaagga aacagccagc gtgccgatcg acgcgccgac
      721 cggcgtgacg cccgcggtgc tcggcgacaa tgtcttcttt ggcaccgagg cgggcgtgtt
      781 ttttgcgatc gattggaagg aggccagggt ttcgtggaaa aaggaagatt ccaatggctc
      841 ccaatcgatt cgtagctcgc ccgcggtgca gcccggcatc gtcgtcgtcg gcagccgcaa
      901 tcgcagcgtg caggcgttcg acccggccac gggaaaggaa ctgtggacct tcgccagcaa
      961 acagcggatc gacagctcgc cggtgatcgt cggcaatcgc gtccttgtcg gcgccgccga
     1021 cggccggctg tacgccctcg acattaaaac cggccagccg acgtgggaat atcaagccac
     1081 cggcggcttc agcggctcgc ccgcgattgc tgacggcaag ctggtcatcg cgagcgaccg
     1141 cggcgtggtg tactgcttcg ggaagaagag ttaggagtgc gagaatggca agcagcgttg
     1201 ccacgggcga aaaactgatg acgatcgagc agtttgcggc gattccccac gacgatcgtc
     1261 agcgagagct ggtcaagggc aaggtggttg aaatgaatct tcccaagccc aaacacggct
     1321 tcatttgcgg cgagattgcg ggattgcttc gcgagtttgt aaagcctcgc aagttgggcc
     1381 gggtctttag caacgactcg ggagtgatca cggagcgaga tccggactcc ttgcgcggcc
     1441 cagacgtggg attttatagt ttcgcacgcg ttccggcggg acccattccc gacggttatt
     1501 atgctgtcgc tccggagatc gcctttgacg ttctttcccc cgacgatgtc tgggcgcgga
     1561 tgctggaaaa ggtcgcagaa ttgctagatg ccggcgtcgt cgtcgtttgt gtcgtggatc
     1621 cccgcgatga aacggtccag gttcatcggg ctgatcaacc gatccagtat ctgcagcgcg
     1681 gcgatgagtt aaccgttccc gatgtcctac cgggattctc ctgcaaagtg tctcaaatct
     1741 ttgaaatctg agtgaccact gaagccacca aaaccgaagt cggcagctat ttcatcgcca
     1801 actatccgcc gttctcgcag tggtcggtcg agcagttgcc ggagctggaa aaggcgctgc
     1861 actcgccgcc gaaggcggtt ccacttggcc tctacctgca catccccttc tgccgcaagc
     1921 gctgcaagtt ctgctatttc cgcgtctata ccgataagac cgcgccggat gtcgagcgat
     1981 atgtccaggc cctctcgcac gaaatcggac tggtcagcca attgccggtg atggggggcc
     2041 ggccgttccg cttcgtctat ttcggcggcg gcacgccttc ctttctctcg gccaaacagc
     2101 ttacgtcgct tgtcgatcgc ctgcggcagc atgtgaactg ggaccatgcg gaagaggtca
     2161 ccttcgaatg cgaaccgggc acgctttcag agccgaaggt caagacgctg aaggagctag
     2221 gcgttacgcg gctcagcctg ggcatcgaga acttcagcga cgcgatcctg gaggaaaatg
     2281 gccgggcgca cctctccgcc gaggtctata aatcgtggga ctggatccag gccgccggct
     2341 ttccgaacgt gaacatcgac ctgatcagtg gcatggtcgg cgagacgtgg gagaactggc
     2401 gtgagaacat caagaagacg atcgacctct cgcccgacag cgtcacgatc taccagatgg
     2461 agctgccgtt caacacggtc tattccaagg acatcctcgg caaccacgtc gagacccctg
     2521 tcgctgactg gccgaccaag cgggcctggc tcaattacgc ctacgacgag ctaatctcgg
     2581 ctggctatca cgtgtcgagc gcctacacag tgg
//
LOCUS       DASUGB010000536         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_200403, whole genome shotgun sequence.
ACCESSION   DASUGB010000536 DASUGB010000000
VERSION     DASUGB010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200403"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="VFV87_10325"
     CDS             <1..1654
                     /locus_tag="VFV87_10325"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HEX5104197.1"
                     /translation="AHPFELVSVDVSEEVRRRAALWSKILPFVLMVWALTGAFYPAID
                     LCAGEKERGTLETLLSSPAARSEIVWGKLLTVMTFSMATSLLNLLSMTATGAFIISQI
                     QRMGAHSLQLDIGPPPLFALFWLVLALIPISALFGALSLAVAAFARSSKEGQYYLMPL
                     LMISLPLMMLTMLPNTELDLGFALIPLSGLMLLLRTLIEGQYAEAARYSVPVIGVTAG
                     CCLLAIRWAIDQFNNESVLFRESERLDLGLWLRHLVRDREDLPTVGEAVLCGVLLLVI
                     KFFASFVAPMPKDWNAFVVTTVVLQIALIATPACLMAIMLTRRPAKSLSLGSPSFLIT
                     IPAAGLLALLLHPAAVWLSHGIQHLYPINPQLEPQLVELEGIIARAPLWQVLLVAALT
                     PAICEELAFRGFILSGLRRMGHKWGAIAVTSLFFGLAHGILQQSLSAFFVGIVIGYIV
                     VKTGSLWPGMLYHLVHNSLGVLLSRLTPETLGNSELLQPIFMTGDEIGIVYRVPFTIG
                     AALAAALILLWFKSLPYHPSAEERLQKALDHQSVGSPARSAA"
     gene            complement(1675..2670)
                     /locus_tag="VFV87_10330"
     CDS             complement(1675..2670)
                     /locus_tag="VFV87_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104198.1"
                     /translation="MFAQRGFQAAVCVQLIVWGTCAVQAEVPRLAPPVKDTLQEVLDR
                     IHHHAASDEWQKEGWKDTAIEAWLDKLIAAIAKGTDRPGFQLPVRLADVKPAELRFRK
                     NGLLIGKDVKALGLENSVVLADGDIEATLPRNCVLVARGVVSISGARNCVIVSGCCVT
                     GTHDGEPRGADGGSLLVSRGWIDLETAYGSTIVAGLGMSIGRSQEARFVNAEVPPMQA
                     GFDWHRGSKSVKILDLPLESALLHLMSEKIRLVGVIQGPSGNDPLTRRRSAAASGVVF
                     RLDDRRYVADLNEAIVDEVGLPVEALRGWTLSHATDRIAIFRNGDQEILVRGQAN"
     gene            2796..3086
                     /locus_tag="VFV87_10335"
     CDS             2796..3086
                     /locus_tag="VFV87_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104199.1"
                     /translation="MERFSAKDQPRTRLGCGFLLLATVITCVMLTINGLIVTNAYYTI
                     VVSTPPGTIHPRVGQAIVFLGPVLLLFVEWWAFDVATDWLHPRSRQERAAKS"
     gene            3154..>5042
                     /locus_tag="VFV87_10340"
     CDS             3154..>5042
                     /locus_tag="VFV87_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5104200.1"
                     /translation="MRKPVVRMAMIAACFAAWFTAAAAQDEDEESPYRSGFIATYSSG
                     ADQKAVRIDETVAFDWADAAPDSRLAAGPFSAVWSGRLWTQTPGVHRIAAYVQGRVSV
                     TLAGNRIIEGSADQPQWLVSEPLELEFDRHPLEIAYERKGDGGRLALFWSGPNFVLEP
                     IPERFLMHDRESSPSTDFERGRQLAAALRCIACHRDEAQPDDAMPAPALDRLSGNIER
                     EWLVEWLSSPLSPRGRGARGEGAANEVKVARRMPHFGLSQEEAETVTTWLTRLPVEEG
                     RQIEQKGAKDVRKAGKEKSSKEKKPQPSAEEGERLYLTLGCLACHQHGDLGESGLFGG
                     GDLTNIAAKRPAEFFARWLTDPASLNKHHRMPVFELSADERTSLALWLAKQRAVGNAL
                     RGVPPGDVARGEKLIQQFRCAACHTLPGVKDGSRNSFAKQLAAASDWTRSCAGESDAK
                     AQRPGYRLAEDDRSALKTYFSSPAPAAVPGTPQRAFPTARALPPSPQAAGRDLLVQLN
                     CLACHQREGIDRQVNSLPARLQDKLLAVAEAHSELGKLIPAMTPPALNSIGDKLHDAA
                     LAAAIRREGPPHRPYLLARMPKFNLSDDQLSALVAYFTTADRVPPIPNELAAAIQNPK
                     SKIQNA"
BASE COUNT          887 a         1690 c         1508 g          957 t
ORIGIN      
        1 agcccatccc ttcgagctgg tcagcgtgga cgtgtccgag gaagttcgcc gccgggccgc
       61 gctgtggtcc aagattctgc ccttcgtgct catggtctgg gcgctgaccg gggcgtttta
      121 tcctgccatc gacctctgcg ccggcgaaaa ggaacgcggc acgctggaaa cgctgctttc
      181 cagcccggct gcccgcagcg agatcgtctg gggcaagctg ctgacggtga tgaccttcag
      241 catggccacg tcgctgctca acctgctcag catgacggcc accggcgcgt tcatcatctc
      301 gcaaatccag cggatgggcg cccacagcct gcagctcgat atcggcccgc cgccgctgtt
      361 tgccctgttc tggctggtgc tggccctgat tccgatttcg gccctcttcg gcgcgctttc
      421 gctggcagtc gccgccttcg cccgcagctc gaaggaaggg cagtactacc tgatgccgct
      481 gctgatgatt tcgttgccgc tgatgatgct gacgatgctc ccgaacaccg agctcgatct
      541 gggcttcgcc ctcatcccgc tcagcggcct gatgctgctc ctgcggacgc tcatcgaagg
      601 acaatacgcc gaggcggccc gctacagcgt gccggtcatc ggcgtcaccg ccggctgttg
      661 tctgttggcg atccgctggg cgatcgatca gttcaacaac gaatcggtcc tgttccgcga
      721 aagcgagcgc ctcgacctgg gcctctggct gcgacatctg gtacgcgacc gcgaggacct
      781 gcccaccgtg ggcgaagcgg tgctctgcgg tgtgctgctg ctcgtcatca agttcttcgc
      841 ctcgttcgtc gcgccgatgc ccaaggactg gaatgcgttt gtcgtcacga cggtggtgct
      901 acagatcgcc ctgattgcga ctcccgcctg cctcatggcg atcatgctca cgcgccggcc
      961 cgcaaaatcg ctctcgctcg gcagtccgtc gttcctcatc accattcccg ccgccggatt
     1021 gctggctctg ctcctccatc cggctgcggt gtggctcagc cacggcatcc agcacctcta
     1081 tccgatcaac ccgcagcttg agccgcagct tgtggagctg gaagggatca tcgcccgagc
     1141 gccgctctgg caggtgctcc tcgtcgccgc cttgacgccg gccatttgcg aggagctggc
     1201 cttccgcggc ttcatcctct ccggcctgcg gcggatgggg cacaagtggg gcgcgatcgc
     1261 ggtgaccagc ctgttcttcg gcctcgcgca cggcatcctg cagcaatcgc tctcggcgtt
     1321 tttcgtcggg atcgtgatcg gctacatcgt cgtcaagacc ggcagcctgt ggccaggaat
     1381 gctgtaccac ctggtccaca acagcctggg cgtattgctc agtcggctga cgcccgagac
     1441 gctcggcaac agcgagttgc tccagccgat cttcatgacc ggcgacgaga tcggcatcgt
     1501 ctatcgcgtg cccttcacga tcggcgctgc ccttgccgcg gcgctcatcc tgttgtggtt
     1561 caagagcctg ccctatcatc cctccgccga agagcggctg cagaaggcgc tcgatcatca
     1621 atcggtcggt tctccagcgc ggtccgccgc ctagctgcct ggacaatcgc cagctcagtt
     1681 cgcttgcccg cgcacgagaa tctcctgatc gccgttccga aagatggcga ttcgatcggt
     1741 cgcgtgcgat agcgtccagc cgcgcagtgc ttccaccggc agaccaactt catcgacgat
     1801 cgcttcgttc aagtcggcaa catagcgccg gtcatccagg cgaaaaacga cgccggaggc
     1861 tgcggcagat cgtcgccggg taagcgggtc gttcccgctc ggaccttgaa ttactccaac
     1921 cagccgaatt ttctcgctca tcaggtgcag cagcgcggac tccagcggga gatccaggat
     1981 tttcacgctt ttggaccctc ggtgccaatc gaatcccgcc tgcatcggcg ggacttccgc
     2041 attgacgaac cgcgcttcct gcgacctgcc aatgctcatt ccaagtcccg ccacgatcgt
     2101 cgaaccgtac gccgtttcga ggtcgatcca gccgcggcta acaagcaaac tgccgccgtc
     2161 cgcccccctg ggctcgccat cgtgtgttcc tgtgacgcaa caaccagaga caatgacaca
     2221 gttgcgggcg ccggagatcg acacgactcc gcgggccacg agaacgcaat ttcgcgggag
     2281 ggttgcttca atatctccgt cggccagtac gacggagttt tccaatccca gcgccttgac
     2341 gtcttttccg atgagcaacc cgttcttgcg aaacctcaat tccgctggct tcacgtcagc
     2401 tagccgaacc ggcaactgga aaccagggcg gtcggttccc ttcgcgatcg cggcaatcag
     2461 cttgtcgagc caggcttcga tcgctgtgtc cttccagccc tctttctgcc attcgtcgct
     2521 ggcggcgtga tggtgaatcc ggtcgagcac ttcctgcaat gtatccttca cgggcggcgc
     2581 caggcgcggc acttcggctt gcaccgcaca cgttccccaa acgatcaact gcacgcacac
     2641 cgccgcctga aacccacgct gcgcgaacat cggattgctc ctgatcgatg gatcgatcgt
     2701 tgccgccccc gctggtcggt tatcataacg cttcgggaaa acgccctgca atcttctcat
     2761 cgtcgcctcg gacagcccct gccattcacc cgtctatgga acgcttctcc gcaaaggacc
     2821 aacctcgcac tcgcctgggc tgtggatttc tgctgctggc gacggtcatc acctgcgtca
     2881 tgctgaccat caacgggctg attgtcacca acgcctatta cacgattgtc gtttcgacgc
     2941 cgccgggaac gattcatccc cgagtcgggc aggcgattgt gtttctgggg ccggtgctgc
     3001 tgctgtttgt cgagtggtgg gccttcgatg tggcgaccga ttggctgcac ccccggtcga
     3061 ggcaagagag ggcagccaaa agctgaatcg ccgcggcggc tgtggtaggg tgggacggca
     3121 gaggacaaga cagaggacca ggctggaggt cgtatgcgaa agccggttgt gcgaatggcg
     3181 atgatcgctg cctgctttgc agcgtggttc acggcggcgg cggctcaaga cgaggacgag
     3241 gaatcgcctt atcggtcggg tttcatcgct acctattcct cgggcgctga ccaaaaggca
     3301 gttcgcatcg acgaaacggt tgccttcgac tgggcagacg ccgcgcccga ttcgcgactc
     3361 gccgctggac ccttttctgc cgtctggagc ggccggctat ggacgcagac gccgggcgta
     3421 catcgaatcg ccgcgtacgt ccaagggcgc gtaagcgtca cgctcgccgg caaccgcatt
     3481 atcgagggca gcgctgatca gccgcagtgg ctggtctctg agccgctgga gttggaattc
     3541 gaccgccacc cgctggagat cgcgtacgaa cgcaagggcg acggcgggcg actcgccctc
     3601 ttttggtccg ggccaaactt cgtgctggaa cccatacctg aacggttcct gatgcacgac
     3661 cgcgaaagct ccccttccac cgacttcgag cgcggccggc agcttgccgc cgccctgcgg
     3721 tgcattgctt gccatcgcga cgaagcccag ccggacgacg cgatgcccgc gccggctctc
     3781 gatcgcttgt cgggaaacat cgagcgcgag tggctcgtcg aatggctctc atctcccctc
     3841 tcccctcgtg ggagaggggc aaggggtgag ggggcagcaa atgaagtaaa ggttgctcgt
     3901 cgaatgccgc acttcggcct gtcgcaagaa gaggcggaga ctgtcacaac gtggctcacg
     3961 cgcctgccgg tggaagaagg ccggcagatt gaacagaagg gcgcaaagga cgtcaggaag
     4021 gccggcaagg agaagagcag taaagaaaag aaaccccagc cgtcggccga ggagggcgag
     4081 cggctgtatt tgacgctcgg ctgcctggcg tgtcatcagc acggcgactt gggcgagagc
     4141 ggcctgttcg gcggcggcga tttgacgaac atcgccgcca agcggcctgc ggagttcttc
     4201 gcccgctggc ttaccgatcc ggcaagcctg aacaaacacc atcggatgcc ggtatttgag
     4261 ctgtccgccg atgagcggac ttcgctggcg ctgtggctcg caaagcaacg cgctgtaggg
     4321 aacgccctcc gtggcgttcc gccgggcgac gtcgcccgcg gcgaaaaact cattcagcaa
     4381 ttccgctgcg ccgcctgcca cacgctacca ggagttaaag acggctcgcg aaattccttc
     4441 gccaagcaac tggccgccgc cagcgactgg acgcgctcct gtgctggcga gtcggacgcc
     4501 aaggcccagc gaccaggcta tcggctggct gaggatgatc gttcggccct gaagacgtat
     4561 ttctcgtcgc cggctcctgc ggcggttccc gggacgccac agagggcgtt ccctacagcg
     4621 cgagcgttgc cgccatcgcc acaggccgct ggtcgcgatc tgctcgtcca gctcaattgc
     4681 ctcgcctgcc accagcggga aggaatcgac cggcaagtca actcgctgcc ggcacgactg
     4741 caggacaagc tgctcgctgt ggccgaagcg cacagcgaac tcggcaagct catccccgcg
     4801 atgacgccgc cggccctgaa cagcattggc gacaagctgc atgacgccgc cctcgccgcc
     4861 gcgatccgcc gcgaaggccc gccgcatcgg ccgtatctcc tcgcgcggat gccgaaattc
     4921 aacctcagcg acgaccaact ctcggccctg gtcgcctact tcacgacagc agatcgagtt
     4981 ccacccatcc caaacgaact cgccgccgca atccaaaatc caaaatccaa aatccaaaat
     5041 gc
//
LOCUS       DASUGB010000537         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_894466, whole genome shotgun sequence.
ACCESSION   DASUGB010000537 DASUGB010000000
VERSION     DASUGB010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894466"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFV87_10345"
     CDS             complement(<1..1219)
                     /locus_tag="VFV87_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104201.1"
                     /translation="MTAEVLQLGPRITCLPVIHGSGEFALAVRRLMLERAFDCVAVPL
                     PASFQADVERGIEQLPTPTIVTQAEPPRYVTEWSPERDDDSDLEDDLEASGGDDGGPT
                     HSYVPIDPCQPVIAALRSAISEHIPRAFIDLETARFQPNTAVLPDAYALKQVAPERFA
                     AAVLPAIERPQVSQVRERIVSMAARLRQLERKHQSILFVCSVLDWPWIREAYVEQTEP
                     AADDDLVEETNLYQPDERTLLFMLGELPFITGLYERARAELEDDENLSIDGVKELLIA
                     ARERYRADLKRRARKITPHLLRQCLKYIRNLSLFERRFTPDLYTLVIAAKQTAGDQYA
                     LHVAETAREYPLAPSADRSSRSELPAVSLGVDRARLPDGEIVRLVSRLPGPPMVWRSC
                     ELKRRPERREREQW"
     gene            complement(1341..1820)
                     /locus_tag="VFV87_10350"
     CDS             complement(1341..1820)
                     /locus_tag="VFV87_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104202.1"
                     /translation="MSNPSSRFDGVKAAFDKMWKRSHDQFSVAQQQAIQITQRIKESF
                     GDNGSIFACVFVNHHYEPVEPAGAVDYDSEKRMPYFCVGVWIQSHDCTFVRIFTIHFA
                     RLNNERWEILEIDGKQISEPDGIPKIVQHMELQLLKDAEGSVFVKDEPKRYIERKRQ"
     gene            complement(1870..2100)
                     /locus_tag="VFV87_10355"
     CDS             complement(1870..2100)
                     /locus_tag="VFV87_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104203.1"
                     /translation="MFRYQIALPMAFTGQHPVIEVDHPDDKPLYSEVVVTLGQDEHIN
                     LGGGSMHLSAGSQVSGIVVGKQALPQGGNANP"
BASE COUNT          402 a          703 c          622 g          429 t
ORIGIN      
        1 gccattgctc gcgctcccgc cgctcgggcc gccgcttgag ctcgcagctc ctccacacca
       61 tgggcggccc cggcaatcgg ctgacgaggc ggacgatctc gccgtccggc aagcgggccc
      121 gatcgacgcc gagcgacacc gccggcaact cgctccgtga gctgcgatcc gcactcggcg
      181 cgagcggata ctcccgcgcc gtttccgcca cgtgcagcgc atactggtcc ccggccgtct
      241 gcttggccgc gatcactaac gtataaagat cgggcgtaaa tcgccgctcg aagagcgaca
      301 ggttgcggat gtacttcagg cactgccgca acagatgcgg cgtgatcttc cgcgcccgcc
      361 gcttgaggtc ggcccggtat cgctcgcggg cggcaatcag cagctccttc acgccgtcga
      421 tcgacaggtt ctcgtcgtcc tccagctcgg cccgcgcccg ctcgtagagc ccggtaatga
      481 acggcagctc ccccaacatg aacaacagcg tccgctcgtc cggctgatag aggttcgtct
      541 cctcgacgag gtcgtcgtca gccgctggct cggtctgctc gacatacgcc tcgcgaatcc
      601 acggccagtc gaggaccgag cagacgaaga ggatcgactg atgcttccgc tcaagctgtc
      661 gcaggcgggc ggccatcgag acgatccgct cgcgcacctg gctgacctgt ggccgctcga
      721 tcgccggcag caccgccgcg gcaaaccgct cgggcgcgac ctgcttcagg gcgtacgcat
      781 ccggcagcac cgcggtgttc ggctgaaagc gggctgtttc gagatcgatg aatgcccgcg
      841 gaatgtgctc gctgatcgcc gaccggagcg ccgcaatcac cggctggcag ggatcgatcg
      901 gcacgtagct gtgcgtcggc cccccgtcat cgccgccgct ggcttccaag tcgtcctcca
      961 agtccgaatc gtcgtccctt tccggcgacc actcggtcac ataccgcggc ggctcggcct
     1021 gcgtcacaat cgtcggcgtc ggcaactgct cgatcccccg ctcgacatcg gcctgaaacg
     1081 acgccggcag cggcaccgcc acacagtcaa acgcccgctc caacatcagc cgccgcaccg
     1141 ccagcgcaaa ctccccgctg ccatgaatta ccggcaggca ggtgatgcgg ggaccgagct
     1201 ggaggacttc cgcggtcatt cattcatatc cgcaaaatta ttggtgctgg ctcttttcgg
     1261 cccccgaacc tctgaaatcc agccaggggt agttgggtgg cgctttttga gaaaaagcgc
     1321 ccccgaaccc gactcagagc ctactgccgt ttccgctcaa tatagcgctt cggctcgtct
     1381 ttgacgaaga cgcttccctc ggcgtctttt agcagttgca actccatgtg ctgcacaatc
     1441 ttggggattc cgtccggttc ggaaatctgc ttgccgtcta tttcaaggat ttcccatcgc
     1501 tcgttgttca atcgcgcgaa atggatcgtg aagatacgga caaaggtgca gtcgtgggac
     1561 tgaatccaga cgccgacgca gaaatagggc attcgctttt cgctgtcgta gtcaactgct
     1621 cctgctggtt caaccggctc gtaatgatga ttcacgaaaa cgcaggcgaa aattgaaccg
     1681 ttgtcgccaa aggactcctt gatccgttgt gtaatctgaa tcgcctgttg ctgtgccact
     1741 gaaaactggt cgtggctacg cttccacatc ttgtcgaaag cggccttaac gccatcgaat
     1801 cgagaactcg gattgctcat ggcttgatct ccatcgaaaa tcgcggcaga tcacgaaaac
     1861 ttgcggggct taagggttgg cgttgccgcc ctggggaagc gcttgttttc cgacgacaat
     1921 tccgctcact tgggagccag cggacaggtg catcgagccg cctcccaggt taatgtgctc
     1981 atcctggcca agcgtaacca ccacctcgct atacaatggc ttatcgtcgg ggtggtcaac
     2041 ctcaattacg gggtgctggc cggtaaacgc catcggcaag gcaatctgat agcggaacat
     2101 tttcggctat tcccaacgag gttgcgggtt agtcgggtat tctatgcgga ctgggg
//
LOCUS       DASUGB010000538         6287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_616022, whole genome shotgun sequence.
ACCESSION   DASUGB010000538 DASUGB010000000
VERSION     DASUGB010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616022"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2697)
                     /locus_tag="VFV87_10360"
     CDS             complement(<1..2697)
                     /locus_tag="VFV87_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104204.1"
                     /translation="MKTTQRPTTRSRNQRSIREARLSAARLRRRALFVERLEERMLLA
                     TIVWDGEGGDNAWGNAANWFDETNGVNNVLPAAADDVEIRAPFAGQVISLVSGALGTT
                     VSINSLQSASGLLIQAGSHAPTSLLIASTAQLDGPVSLVSGNFGGGGALTIGDRLTWS
                     GGSLAGGGTLIVAPGATLAFDSPGSPFSTGSWTIENQGTLDWIRGGLSTSDPMTINNR
                     PGGLFNVRDGNSFGSGNSGRIINNEGTLRKSEGMGRSQIGMIFNNTGSVEVQAGTLAL
                     VKGGVSSGNFSFDAGTTLELRGGDDFFNNSHTITLGPGDSIAGEGRLLIADRPITIQG
                     TGTVEIASLEISTRASSRFNLNLDASVPKVLFASGFIGGTGTLTVTESMSWTGGQLNE
                     GGTVLVAPDATLTLDATSVGITTGGGNIDNHGTFRWLRGAINASSSSRLTLHNRAGAL
                     FDIQSNAAAGAASSVVHNAGIIRKSAGTGRSELRMAVNSTGSVEVQTGTLALQGGGNS
                     TGNFSFAAGTTLELWGNFPGSPNVPNTITLDSASSITGSGRLLIGGGGVTIGGSGLMD
                     VQQLELQGNFVDLDLDLDTSVPVVLVTAGRLGGSGTLTVTENMTWSNSSLADGGTNVI
                     GPSATLTISGSTSINDNDSGASPTHTIDNHGTIQWLSGDIFIGDPLVLRNRPGAVFDI
                     RGDATLGHQGSLINEGTLRKTAGAGTTAINTAVSNTGAIEVHSGTLRLQQPVSNWSSA
                     QSALIGGTWLVSTADPLVSTATLRLDGVSPFANNRAKITLDGPGTSLINQSSVDLLRD
                     LRQIDPAASLTIENGRDYSPAAGDFTNGGTLTVGPESTVSVSGQYIQSAEARLAIGLG
                     GDAASGLFGHLAATGNANLDGTFELRLYDGFGPTTGQSWQVMS"
     gene            2932..3546
                     /locus_tag="VFV87_10365"
     CDS             2932..3546
                     /locus_tag="VFV87_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104205.1"
                     /translation="MAPDSPGAETVWSELLREARAGEQLAIGELIETWRTYLFHLVET
                     EITDDLRQKVGVSDLVQSACLDIHLRFGDFRGSTVEEWRVWLKSMMRHDLQDARRRFV
                     DAQRRDLRRERSLEAGERPTHDVSDPAPSPRASLIAAEESAALQAALARLPDDYRLVL
                     RLRNWDCLPFAEVGKQMDRTEEAARKLWSRAIVRLQEEFPNPPG"
     gene            3560..>6287
                     /locus_tag="VFV87_10370"
     CDS             3560..>6287
                     /locus_tag="VFV87_10370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5104206.1"
                     /translation="MNPAPSPQPERLDEQLVAYHEALLAGASPPPDSDALPPALEQCL
                     KLVDRVRRQKRTDDAERLFGQLSGENEERGSATARPAEGNIARDGDESPRQIGRFRIE
                     RRIGEGGFGVVFLAVDPVLNRRVALKLPRPETIISSSWRSRFLREAEAAALLDHPHIV
                     PVHEVGEAGSLCYIVQAWCDGVSLDRWLAAHGACRSPLAAAELVATLAETVQHAHSRG
                     VLHRDIKPSNILLESPASPSDEQPAGADQGEPGTVDTKSELTMGDTAAGPPAAARDIG
                     ANRSRADAIPEEQLSRVARLADFGLARLIERGAEQTRSGSVLGTPSYMAPEQAEGRLR
                     DVGAAADVYSLGAVLFELLAGRPPFREVSLLATLQAVREREPPSLRSLRGEVPIDLDA
                     ICLKCLEKNPQRRYATAGALAEDLRRLPRGEVVSARTISSAERLLRWCRRNRTLSGLA
                     AAVLLLVTALLVGTTTAAIWLAQAQQKTLDSLEAEIVAKDKATASEELALRAVFEARL
                     AEARASRTGEQPRGRTEALDALAKAAAHIERLKLDKTEILRLRNEAIAAMALVDLRLD
                     RQWPGHPPRYQFASTGFDAEVERYAALEPDGQIAIRRLKDNALVLRVGGLGRLDRKPC
                     IQFSPNGRFLAVRGINEEQALRVQLWELSRVEQPILDEPAGGELADRSIEFSPDSQRL
                     AFAGADGTVQFFGIETRKMARPLALGWSPDWLRFSPSGDRLATVTDQADVVLFLSPAT
                     GQGLALPLKHAASVHDAAWSSSGQLLATACRDWRVHVWDAAAGRRLASCDRQHGTVHH
                     VAFDPTDDLLMSSGWNGTTIWDPLSGRALVRSIEGGVRFSRDGRWLSRAIPGVVAGRW
                     EVAPRREYFTLSGHLRDPYVYALAFHPGGRLLASAAAGDGIRLWDVAGRREVA"
BASE COUNT          972 a         2022 c         2161 g         1132 t
ORIGIN      
        1 gctcatcact tgccacgatt gccccgtcgt cgggccgaag ccgtcgtaga gtcgcagttc
       61 gaatgtgccg tccagattgg cgttgcctgt cgccgcgaga tggccgaaga ggccgctcgc
      121 tgcgtcgcct cccaggccga tcgccagccg ggcctcagcc gattggatgt actggccgct
      181 cacggaaacc gtgctctcgg ggccaacggt caaggtgccg ccgtttgtaa agtcgccagc
      241 cgcgggtgaa tagtcgcggc cgttctcgat cgtgaggctc gccgccggat cgatctgccg
      301 caggtcgcgc agcagatcga cgcttgactg gttgatcagc gaagtgcccg gtccatcaag
      361 cgtgattttg gcccggttgt tcgcaaacgg gctgacgcca tccagccgca gcgtggccgt
      421 gctgacgagc ggatccgcgg tggaaacgag ccaggtgccg ccgatcaggg cgctttgggc
      481 gctgctccag ttgctcaccg gctgttgcag gcggagcgtt ccgctatgga cctcgatcgc
      541 gcccgtatta ctcaccgcag tattgatagc ggttgttccc gcgccggcgg tcttgcgcag
      601 cgtgccttcg ttgatcagcg agccttggtg gcccagggtg gcatcgccgc ggatatcgaa
      661 aacggctccg ggacgattgc ggagcacgag cggatcgcca atgaatatgt cgccggagag
      721 ccactggatc gtgccgtggt tgtcgatcgt gtgcgtgggc gatgcgccgc tgtcattgtc
      781 gttgatgctc gtgctgccgc tgatggtaag cgtggcgctg gggccgatca cgttggtgcc
      841 gccgtcggcc agagagctgt tggaccaggt catattctcg gtgacggtta gcgtgccgct
      901 gccgccgagg cggcccgccg tgaccagcac taccggtacc gacgtgtcaa gatcgaggtc
      961 cagatccacg aaattccctt gcagctcgag ttgttgcaca tccatcaatc cactgccgcc
     1021 aatcgtgacc ccgccgccgc cgatcaagag gcggccggaa cccgtgatcg aacttgcgga
     1081 atcgagcgtg atggtgtttg gcacgtttgg ggaaccggga aagttgcccc agagctcgag
     1141 tgtcgtcccg gcagcgaacg agaagttccc cgtgctgttt ccgccgccct gaagggccag
     1201 tgtgcccgtc tgcacttcga ccgatccggt gctgttcacg gccattcgca gctcgctgcg
     1261 cccggtgccc gcgctcttgc ggatgatgcc cgcgttgtgg accacgctgc tcgccgcgcc
     1321 cgccgccgcg ttcgactgga tgtcgaatag cgcgccggcc cggttgtgca gcgtcagccg
     1381 gctgctggag gaggcgttga tcgcgccgcg cagccagcgg aacgtgccgt ggttgtcgat
     1441 gtttcccccg ccagtggtga taccgacgga cgtggcatcg agcgtgagcg tcgcgtccgg
     1501 agcgacgagc acggtcccac cctcgttgag ctgtccgccg gtccaactca tgctttcggt
     1561 gaccgtcagc gttccggttc ctccaataaa cccgctggca aatagcacct tgggaaccga
     1621 ggcatccaga ttcaagttga aacgcgagct ggcgcgcgtg ctgatttcca gcgaagcgat
     1681 ctcaaccgtg ccggttccct ggatcgtgat tggccgatcg gcaatcagca ggcggccttc
     1741 gccggcgatg ctgtcgcctg ggccgagcgt gatagtgtgg gagttgttga agaaatcgtc
     1801 gccgccgcgc agctcgagcg tagtgcccgc gtcgaacgag aagttcccgc tgctgacgcc
     1861 gcctttgacg agggcgagcg tgccggcctg gacttccacc gaaccggtgt tgttgaagat
     1921 catcccgatt tgcgagcggc ccattccttc gctcttgcgg agcgttcctt cgttgttgat
     1981 gatccgcccg ctgttgccgc tgccgaacga gttgccgtcg cgaacattga acagcccgcc
     2041 tggacgattg ttgatcgtca tcgggtcgga agtgctgagg ccgccgcgaa tccagtcgag
     2101 cgtcccttgg ttttcgatcg tccacgaccc ggtgctgaag gggctgcctg gcgagtcgaa
     2161 cgccagggtc gcgcccggag cgactatcag cgtccctccg ccagcgaggc tgccgccaga
     2221 ccacgtcagc cggtcgccga tcgtcaaggc gccgccgccg ccgaaattgc ccgaaaccag
     2281 gcttaccggc ccatccaact gagcagtgga agcaatcagc agcgaggtcg gcgcatgact
     2341 gcccgcttgg atgagcagcc cggacgcact ctggaggctg ttgatgctga ccgtcgtgcc
     2401 caaggcgccg gaaacgagcg aaatcacctg gcccgcgaac ggcgcgcgga tctcgacatc
     2461 gtcggcggcg gccggcaaga cgttgttgac cccgttggtc tcgtcaaacc agttggcggc
     2521 gttgccccag gcgttgtcgc ccccctcgcc gtcccagacg atcgtggcca gcagcatccg
     2581 ctcctccagc cgctccacaa acagcgcgcg gcggcgcaag cgggcagccg agagccgggc
     2641 ctcgcggatc gatcgctggt ttcgcgagcg agtcgtgggt ctttgggtgg ttttcatcgc
     2701 gctggctccc ggcggattcg cgcccggggt ggaaaagccc gagacggtgc gcgaattcgt
     2761 ccgtaagcga aggcgcgagt tgcggcagga ccggacgaag cgtttcgtgc aattgacggg
     2821 cggccaagtt acagtaggag ggcgtcgaga actcgctggt tttcggctga ttttcctcgt
     2881 ttgggcgtcc ggttttcctt tccttcgcgc ctcgttgatg gggatcgtgc agtggcccct
     2941 gattcccccg gagcggaaac ggtctggagc gagctcctcc gcgaggcccg cgccggcgag
     3001 caattggcca ttggcgagct gatcgaaacg tggagaacct acctgtttca cctggtcgag
     3061 acggaaatca ccgacgactt gcggcagaag gtgggcgtat ccgacctggt gcagagcgcc
     3121 tgcctcgata ttcacttgcg gttcggcgat ttccgcggga gcaccgtgga ggagtggcgc
     3181 gtgtggctga agagcatgat gcggcacgat ctgcaggacg cccgccggcg gttcgtcgat
     3241 gcccagcggc gcgatctgcg gcgcgagcgc tcgctcgaag cgggcgagcg gcccacgcac
     3301 gacgtgagcg atccggctcc gtcgccgcgg gcttcgctta tcgccgccga ggagtccgcc
     3361 gccctgcaag cggcgctcgc ccggctgccg gacgactatc gcctggtgtt gcgactgcgg
     3421 aattgggatt gcctgccctt tgccgaagtg ggcaagcaaa tggaccggac cgaggaggcg
     3481 gcccgcaagc tgtggtcgcg ggcgatcgtg cgtttgcagg aggagtttcc gaacccgccg
     3541 ggataaggca acgcgcggca tgaatcccgc accttcccca caacccgaac gactcgacga
     3601 gcagctcgtt gcctatcacg aggcgctgct ggccggcgcc tcgccgcccc ccgattcgga
     3661 cgcgctgccg ccggcattgg agcagtgcct caagcttgtg gatcgcgtcc gccgccagaa
     3721 gcgcacggac gatgcggagc ggctgtttgg tcagttgtct ggagagaacg aagaacgggg
     3781 ctccgcgact gctcggcccg ccgagggcaa tatagcgcgg gatggcgacg agtcgccgcg
     3841 gcagattggc cgcttccgga tcgagcggcg aatcggcgaa gggggcttcg gcgtcgtttt
     3901 cctggcggtc gatcccgtgc tgaatcggcg cgtcgcccta aaattgccgc ggccggagac
     3961 gatcatttca tcgtcgtggc ggtcgcgctt tctacgcgag gcggaagccg ctgcgctact
     4021 cgatcacccg cacatcgttc ccgtgcatga agtcggcgaa gctggctcac tgtgctacat
     4081 cgtgcaggcc tggtgcgacg gcgtttcgct cgaccgctgg ctggcggcgc acggtgcgtg
     4141 ccgcagcccg ttggccgccg ctgagctggt ggcgacactg gccgagacgg ttcaacatgc
     4201 ccacagtcgc ggggtgttgc atcgcgacat caagccgtca aacatcctgc tggagtcgcc
     4261 cgcctctccg agcgacgagc agccggccgg cgcggatcag ggcgagcctg gcacggtgga
     4321 tacgaagagc gaactgacga tgggcgatac ggctgcgggg ccgccggcgg cggcgcgcga
     4381 catcggcgcc aatcgttcgc gggccgatgc cattccggaa gagcagctca gccgcgtggc
     4441 ccgcctggcc gatttcggtt tggcccggct gatcgagcga ggtgccgagc aaacgcgcag
     4501 cggctcggtt ctcggcacgc cctcctacat ggcccccgag caggccgagg gccgcctgcg
     4561 cgatgtcggc gccgctgccg acgtgtattc gctgggggca gtgctcttcg agttgctcgc
     4621 cgggcggccg ccgttccgcg aggtatcgtt gcttgccacg cttcaagccg tccgtgagcg
     4681 cgagccgcca agtttgcgat cgctccgtgg cgaggttccc atcgacctgg acgccatctg
     4741 cctgaaatgc ctggagaaga atccgcagcg gcgctatgca acggccggcg cgctggctga
     4801 agacctgcgg cggctgcccc gcggagaggt cgtttcggct cggacgattt ccagcgcgga
     4861 gcgcctgttg cgctggtgcc ggcggaaccg caccctgtcc ggcctggccg cggctgtgct
     4921 gttgctcgtg acggcgctcc tggttggcac gaccaccgca gccatctggc tggcccaggc
     4981 tcagcagaaa acgctcgaca gtctggaagc cgagatcgtc gccaaagaca aggcgaccgc
     5041 aagcgaggag ctggccctcc gcgcggtgtt cgaggcgcgc ctcgccgaag cccgcgccag
     5101 ccgcaccggc gagcagccgc gcggccgcac cgaagctttg gacgccctcg ccaaggccgc
     5161 cgctcacatc gaacgcctca agctggacaa aaccgagatc ctgcggcttc gcaacgaggc
     5221 gatcgccgcg atggcgctcg tcgatttgcg gctcgatcgc caatggcccg gccacccgcc
     5281 gcgctatcaa ttcgccagca ccggcttcga cgcggaagtc gagcgatatg cggcgctcga
     5341 gccggatggc cagattgcca ttcgccggct gaaggacaac gcgctggtgt tgcgcgtagg
     5401 cgggctgggc cggctcgatc gcaagccctg catccagttc agccccaatg gccgttttct
     5461 ggcggtgcgc ggcatcaatg aagagcaagc cctgcgggtg cagctttggg agttgagtcg
     5521 ggtggagcag ccgatcctgg acgaacccgc cggcggcgag cttgccgatc gcagcatcga
     5581 gttcagccct gattcgcagc gtctggcctt cgccggcgcg gacggcacgg tgcaattctt
     5641 tggaattgaa acgcggaaga tggcccggcc gctggcgttg ggatggtcgc ccgactggct
     5701 gcgctttagt cccagcggcg accgcctggc gaccgtcacc gaccaggccg acgtcgtcct
     5761 gttcctttcg ccggccacgg gccaggggct ggccttgccg ctgaaacatg ccgccagcgt
     5821 tcacgacgca gcttggagtt cctcgggtca actgctggcg accgcctgcc gcgattggag
     5881 ggtccacgtg tgggatgccg cggcgggccg gcggctggca tcgtgcgatc ggcagcacgg
     5941 cacggttcat cacgtcgcgt tcgatccgac ggacgacttg ctgatgtcgt ccggctggaa
     6001 cggcacaacg atctgggatc cgctcagtgg aagggcgctg gtgcggtcga tcgaaggagg
     6061 cgtccgcttc agccgcgacg ggcgctggtt gtcgcgggcg attcccggcg tggtcgcggg
     6121 gcggtgggaa gtcgctcccc gccgcgagta tttcacgctc agcggccatt tacgcgatcc
     6181 gtatgtctat gcgctggctt ttcatccggg cggcaggctg ctggcttcgg ccgcggcggg
     6241 cgacgggatt cgcctgtggg atgtggccgg gcgccgcgaa gtggctc
//
LOCUS       DASUGB010000539         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1866906, whole genome shotgun sequence.
ACCESSION   DASUGB010000539 DASUGB010000000
VERSION     DASUGB010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866906"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFV87_10375"
     CDS             complement(<1..402)
                     /locus_tag="VFV87_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104207.1"
                     /translation="MNALASQPAEILVVDHDPYTLIGTAAVLDMAGYACHCARDGAAA
                     LKAAARIALDLVICDVHLGEESGVEVCRQLRLAPGMQDVPVMFVSATQLTDIVRRSHE
                     AGAAYFLKKPLDPAVLVELVGKALWLPQQVRS"
     gene            complement(1312..2394)
                     /locus_tag="VFV87_10380"
     CDS             complement(1312..2394)
                     /locus_tag="VFV87_10380"
                     /inference="COORDINATES: protein motif:HMM:NF033579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX5104208.1"
                     /translation="MFPDYAHKFSPKTYTQSQLFACLVLKEFLHRDYRGIEELLKDTP
                     ELAAVIELRKVPDFTTLQKAANRLLQSAAASRLLDMTVRLGKFLGKLDTKAPLAAMDG
                     TGMESRHASAYYVRRKAKGGKSEQKLTYQRFPKAGLVSDCKSHLILAVVPQRGPGPDI
                     THFRRAFDQARRRLRFKTLAADAGYDAEHTHEYARSRGVRTLIPPLIGRPTTKPPAGY
                     WRRTMLRVLPRSRYGQRWQSETVNSMLKRLLGSALRARSYWSQSRETMLRALTLNVMI
                     LRRRQVFDRACQEPLMWKRGLPRERIVASSSCRTVCSAPPGPFERVGLHPHPRDHARR
                     SLRRGAGSGVILLRRLGPGTSRASAR"
BASE COUNT          459 a          828 c          751 g          500 t
ORIGIN      
        1 cgagcgcacc tgctgaggca gccagagggc tttgcccacc agctcgacga gcaccgcggg
       61 atcgagcggc ttcttcagga aatacgccgc ccccgcttcg tgcgagcggc gcacaatgtc
      121 ggtcagctgc gtcgccgaga cgaacatcac cggcacgtcc tgcatgccgg gggccagccg
      181 caattggcgg cagacctcca cgccgctttc ctcccctagg tgcacatcgc aaatcaccag
      241 gtccagggcg attctcgccg cggccttcag tgcggccgct ccgtcgcggg cgcagtggca
      301 ggcgtaaccg gccatatcga gcactgccgc cgtcccaatc agcgtatagg ggtcgtgatc
      361 aacgaccagg atttcggccg gctgcgaagc aagcgcgttc atcgagcgac agtcccacgg
      421 tggcgaggga atttgccgct ccatcggcgc ccgcgagcta accgcgggca ctactccatc
      481 gttgtcgtca tcggcctccg aagctttcag gacccagaaa agcttgcccc ggcgatacag
      541 gcgctagatg cagcgcatcc gcgcgacacc cgcgcaagtc acggcgtgta cgtctccggt
      601 ttgctgtttc gacgtgccct cgttcactgc ccgatcgctg ccggttgcca atattgccca
      661 atgcccgtca agtagcttgg cctggtaata cttctcaaac ctttgccgcc acgggatttg
      721 tgacattacc actggtgtgg atcgggcgtg caatagctcg ttttattagc acgccggcca
      781 gtcgcgattg gggcatttgg cggcgctgag cctcggccgg cccgcttgct ggtttacgcc
      841 ctccgcagct cgccggaggc tgtttttagc cataactcga atccctgccg gacgttgcgt
      901 tggtttatgg caaccggccc cgcgtggtaa cctgcgcgtg cgaaatggcc ccgttttctg
      961 gcacgggcga ctgcgtttgg ttgacaaaaa ccggcaacag gcgggctctt gtaaaccacg
     1021 catttttctc ccccgtccga cgactcacac cctcgcaccc tcacaccatc gcaccctcac
     1081 accatcgcac tctcacacgc tcgcactctc acacccactc gctccctagt tccgcaaaaa
     1141 gccggttgcc tgtcaagcaa ttgcgcgtct cagaacgaga cgagcccgac ttgcgccgcg
     1201 gccggaatct gcaaccgccg ccttgccatg cacttacgcc gccgccgatc gtcgcaagtc
     1261 atttcggaaa ttccgcggcg cgtgacagat caggtccctc cccccgcccg cttatctggc
     1321 agacgccctc gatgttccag gaccaagccg gcgaagtaga atgacccccg atcctgcccc
     1381 gcggcgaagg gagcgccttg catggtccct tgggtggggg tgcaggccga cgcgctcgaa
     1441 agggccgggc ggagcggagc aaaccgtgcg acacgagctg gaagcgacga tccgttcccg
     1501 cggaaggcca cgtttccaca tcaaaggttc ctgacatgct ctgtcgaaaa cctggcgtcg
     1561 tctaagaatc atcacattga gggtcagcgc tcggagcatg gtctcgcggc tttgggacca
     1621 gtaggagcgg gcgcggagtg cggagcctaa caggcgtttg agcatactat tgaccgtttc
     1681 cgactgccaa cgctgcccat agcgcgaacg gggcaggacg cgaagcatgg tccgccgcca
     1741 gtaacctgcg ggaggtttgg tggttggcct tccaatcaag ggcgggatca aagtccgcac
     1801 gccacggctg cgggcgtact catgcgtgtg ctcggcatcg tagcccgcgt cggccgccag
     1861 cgttttaaag cgcaggcgac gccgcgcttg atcgaacgcc cgacggaagt gcgtgatgtc
     1921 gggtcccggt ccgcgctgcg gcacgaccgc cagaatcagg tggctcttgc agtcgctgac
     1981 gagtcccgcc ttcgggaacc gctggtacgt cagtttctgc tcggatttcc cccctttggc
     2041 cttccgccgc acgtaataag cgctggcgtg ccgtgactcc atgccggtgc cgtccatcgc
     2101 ggccagtggc gctttggtat caagttttcc caggaacttt cccagccgca cggtcatgtc
     2161 gagcaggcgg ctggccgctg ctgattgcag caagcgattg gccgccttct ggagggtggt
     2221 gaagtcgggc accttccgca gttcgatcac ggccgcgagt tccggcgtgt ctttcagcag
     2281 ctcttcgatc ccgcgataat cgcgatgcag gaattccttg agtaccaggc aggcaaacag
     2341 ttgcgactgc gtgtaggttt tagggctgaa cttatgagcg tagtccggga acaccagctt
     2401 tccaatcaga tacccggtca ggagaatgct tcgcggagat ttcgacgtag cagccatcta
     2461 catggcgcta cgcatctcat ccaagctggc gttcacttgc tagcagcatg ttttcgacag
     2521 agcatgtcag gaaccttt
//
LOCUS       DASUGB010000540         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_755878, whole genome shotgun sequence.
ACCESSION   DASUGB010000540 DASUGB010000000
VERSION     DASUGB010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755878"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFV87_10385"
     CDS             complement(<1..925)
                     /locus_tag="VFV87_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5104209.1"
                     /translation="MTKLLTEEQEEHYGPNGIGFDPSKLVVKCGPADERDERLVIASL
                     YGATHRDKFNTDESWKRQKFREKFSSLCGCETPEFFEAVEAEIMAKADAADAISDAAS
                     GPLWTPSLVSLADIETKDVRWFWPGVMPEGAITVLDSDPGEGKSTITDDVAARYSAGN
                     SMPPHSAPDGTFAPGNVLILDGEEDVQRTRKPRLVAAGADVNRIYFLRTTTMEDDERF
                     IQLPLDLAMIEQVVVEKEIGLIIIDVLATFIQDGTSMNDDAAMRKLTGPLANMLERTR
                     ATAIVLRHLNKKENLRGMYRGGGSIAIVAAAR"
     gene            complement(922..1263)
                     /locus_tag="VFV87_10390"
     CDS             complement(922..1263)
                     /locus_tag="VFV87_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104210.1"
                     /translation="MSNYIDFNRLATAVRRNAAARGWRGDIYARSDELPDCGTYRQEW
                     EIVDIIGDVADVERGLDCRARVLVRRLARAAVLGPQSESFKLQTARPTTICPSPLDHD
                     AKLAREWRRKR"
     gene            complement(1318..1512)
                     /locus_tag="VFV87_10395"
     CDS             complement(1318..1512)
                     /locus_tag="VFV87_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5104211.1"
                     /translation="MPVVVESVPKLLLTQREAADALGVSERTLFSLTSPRGPIPAVRM
                     GKSVRYSLAALQAWIGEQTK"
     gene            complement(1612..2220)
                     /locus_tag="VFV87_10400"
     CDS             complement(1612..2220)
                     /locus_tag="VFV87_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104212.1"
                     /translation="MIVVGTTATAWLAGLAERWCIMDRSILAALADYLDQLADDVKLS
                     PRLVTEFIARRAKSLEWNLYLVSPDEPSPDDRLGHAVAEAANLCRPLCEYPGEIDDPA
                     TSERLQTAARVIRAELEPLPPPRDNEPSDCYVTLNQMAAIVQKGKRTLERLKTNGKIP
                     APDVAGGGGKHDLWRWSVVRPILETEYERPLPEIFPAARFVG"
     gene            complement(2267..3712)
                     /locus_tag="VFV87_10405"
     CDS             complement(2267..3712)
                     /locus_tag="VFV87_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5104213.1"
                     /translation="MASISTDKRGNRTVQFMGCDRKRRSVRLGPCSLRIAEKWQGHIE
                     RLAIAKKLNHSVERETANWVADLEDPMHEKLAAVGLVEPRAPKIKIGLGAYLDEFVAK
                     RIDVKPATIEVWGQIVRNLKGHFGEGRDPATITEADADDFKLYLIGEKLSPTTVSKRL
                     QFARMFFKSMLKRKLIVVNPFAEVSARAVMRTDRQRYVTPEETKLIMDVCNPEWKVIV
                     ALARYGGLRTPSEPLSLRWQDINWDTLRIVVTSPKTEHHEGKGSRVIPLFPELLKPLR
                     EAYEAAEPGAEFVLPRQRAASLGSRGWRNCNLRTQFERLVKRAGLAPWPRLFHALRSS
                     RETELAKEYPIHVVTSWLGNTPRIAMKHYLMTTDSDFERAAKSGAVSSRTEPHQQETP
                     ANPDDESSRFAGACGTMRDSAIPSCGEDRKRTSAKFAEICAEFAASAANSGAVLDGLA
                     TVVEAWPNLPAELRRDILGLVESAVAGEWVS"
BASE COUNT          687 a         1222 c         1105 g          812 t
ORIGIN      
        1 ctctggcggc cgcgacgatg gcgattgaac cgccgccgcg atacatgccc cgcaagtttt
       61 ctttcttgtt gagatggcgc agcacgatcg ccgtagcccg cgtgcgctct agcatgttgg
      121 ccagcgggcc ggtcagcttt cgcatggccg cgtcgtcgtt catgctggtg ccgtcctgaa
      181 tgaacgtggc cagcacgtcg atgatgatta ggccgatttc tttttcgacg acgacttgct
      241 cgatcatcgc caggtcgagc ggcaactgaa tgaatcgctc gtcatcttcc atcgtagtgg
      301 tgcgtaggaa gtagattcga ttcacgtcag ccccggccgc gacaagacgc ggcttgcggg
      361 tccgctgaac atcctcttcg ccatccagga tcaggacgtt tcccggcgcg aatgttccat
      421 cgggtgctga gtggggcggc atggaattgc cagcgctata gcgggcagcg acgtcatcgg
      481 taatcgtgct cttgccctcg cccgggtcag agtccagaac cgtaatcgcg ccttccggca
      541 tgacgcccgg ccagaaccag cgaacgtctt ttgtttcgat gtctgccagg ctgaccagtg
      601 aaggcgtcca cagcggccca ctcgcggcgt cggagatcgc gtcggccgcg tcggccttgg
      661 ccataatctc agcttcgaca gcctcgaaaa actctggcgt ttcgcaaccg cacaaactgg
      721 aaaacttttc gcggaacttt tggcgtttcc agctttcgtc agtattgaac ttgtcgcggt
      781 gcgttgcccc atagagagaa gcaatcacca gcctttcgtc gcgttcatcg gctggcccgc
      841 acttcacaac cagcttgctc ggatcgaagc caatcccgtt tggcccatag tgctcttctt
      901 gctcttccgt gagaagtttg gtcatcgctt ccgcctccat tcgcgggcca gtttggcgtc
      961 gtgatctaga gggctcgggc aaatcgttgt aggtcgtgct gtttgcaact tgaacgattc
     1021 agattgcggg ccaagcacag ccgcgcgggc cagccttcga acgagcacgc gagcacggca
     1081 gtcaaggccg cgttccacgt ccgcaacgtc gccgatgatg tcgacgattt cccactcttg
     1141 ccgatatgtt ccgcagtcgg gcagctcgtc gctgcgggcg tagatatcgc cgcgccagcc
     1201 tcgtgccgca gcgttgcggc gaacggctgt tgccagccgg ttgaaatcga tataattgct
     1261 catagttcga ctccaagccc gccggccgct ctctcacaat tggccgcgcg ggctttctta
     1321 tttggtttgc tcgccgatcc acgcctgaag cgccgccagc gaataacgga cgctcttccc
     1381 catccgcacc gcggggatgg ggccgcgggg ggatgtaagt gaaaacagcg tacgctcgga
     1441 tacgcccagc gcgtcggccg cttcacgctg ggtcaacaaa agcttgggga ctgactctac
     1501 gacgacgggc atggcgtgcg cctccggtgt ttgagggtgg acgcacgcaa aatacagcac
     1561 gccgccaaga atggcggcgg ttggcggggg ttggcggtta gtggcggtca gctaaccgac
     1621 aaaccgagcg gcagggaaga tttccggcag gggccgctcg tattcggttt caagaatagg
     1681 ccgcacgacg gaccaccgcc acagatcatg cttcccgccg ccgccagcaa cgtcgggggc
     1741 cgggattttc ccattcgttt tcagccgctc aagagtccgc ttgccctttt gtacgatcgc
     1801 cgccatttgg ttcaacgtca cgtagcaatc agacggttca ttgtcacgcg gcgggggcaa
     1861 cggttccaac tctgcgcgga tcacccgggc cgccgtttgc agtcgctcgc ttgtcgccgg
     1921 gtcatcgatt tcgccgggat actcgcacag cggtcggcat aggtttgccg cttcggcgac
     1981 cgcatggccg agccgatcat cggggctggg ttcgtcgggg gaaacgagat acagattcca
     2041 ttcgagagac ttggcccggc gagcgataaa ctctgtgacg agccgcgggc taagcttcac
     2101 gtcgtcggcg agctgatcca aatagtcggc aagcgccgcg agaatagatc gatccatgat
     2161 gcaccacctt tcagccaggc cggccagcca tgccgtcgcg gtggtgccga cgacgatcat
     2221 ggccagcctg tgcgggtgta cggcccgcga gcccagcaat tcgattttaa cttacccact
     2281 caccggcgac agccgactcg accagcccga gaatatcccg ccgcagttcc gccggcagat
     2341 tcggccacgc ctccaccaca gttgccaaac cgtccaaaac tgcaccggaa tttgcagcgc
     2401 ttgcggcgaa ttctgcacaa atctcggcga atttggcact agttcgcttc ctgtcctctc
     2461 cgcatgatgg aatggcacta tcacgcatag tgccgcaagc ccctgcaaat ctagatgatt
     2521 cgtcatccgg gtttgcaggg gtttcttgtt ggtgcggctc tgtgcggctg ctgactgcac
     2581 cgctttttgc agcgcgctca aagtccgaat ccgtcgtcat caggtaatgc ttcatggcga
     2641 tgcgtggcgt gttccccagc cagctcgtca cgacgtggat cgggtattct ttcgccaact
     2701 ccgtttcccg gctcgatcgc aaggcgtgga acagccgcgg ccacggcgcc agccctgccc
     2761 ttttcaccag ccgctcaaac tgggtccgca gattgcaatt gcgccaccca cgcgagccca
     2821 gggacgcggc ccgctgtcga ggcaacacaa actccgcccc tggctcggcc gcttcgtacg
     2881 cctcgcgtag cggcttcagc agttcaggaa acaacgggat gacgcgcgag cccttccctt
     2941 cgtgatgctc cgtcttcggc gacgtcacga cgattcgcag cgtatcccag ttgatgtcct
     3001 gccagcggag tgacaacggc tcggaaggcg tccgcagacc gccgtagcgg gccagggcca
     3061 cgatcacttt ccattcgggg ttgcagacgt ccatgatcag ctttgtctct tctggcgtca
     3121 cgtaacgctg cctgtcggtc cgcatgacag cccgggccga gacttcggcg aacgggttga
     3181 ccacgatcag cttccgcttc agcatcgact tgaaaaacat gcgagcgaat tgcagccgct
     3241 tggataccgt cgtcggcgat agtttctcgc cgatcaggta aagcttgaag tcgtcggcgt
     3301 ccgcttcggt aatcgtggcc ggatcacgcc cttccccgaa atggcccttc aggttgcgga
     3361 ctatctgccc ccagacctcg atcgtggccg gcttcacgtc gatgcgtttg gcgacaaact
     3421 catccagata cgccccgagg ccgatcttga tcttcggcgc ccgaggctcg accagcccca
     3481 cggccgccaa cttttcgtgc atcgggtctt ccaagtcggc aacccaattg gcggtttcac
     3541 gctccacgga gtgattcagc ttcttggcga tcgccagccg ctcgatatgc ccttgccact
     3601 tctcggcaat tcgcagactg cacggaccca gccgcaccga tcgccgcttc cgatcgcagc
     3661 ccatgaattg cacggtgcgg ttcccccgct tgtcagtcga aatgcttgcc atgatgctac
     3721 tgcccccttc ctgattgttg ctctgccacc cagctttcaa agtcgtggtg tatcttcagg
     3781 tcggcgattg gctcgccaag cacccagccg agtgcaatgg ccttgc
//
LOCUS       DASUGB010000541         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2007114, whole genome shotgun sequence.
ACCESSION   DASUGB010000541 DASUGB010000000
VERSION     DASUGB010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007114"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2941)
                     /locus_tag="VFV87_10410"
     CDS             complement(<1..2941)
                     /locus_tag="VFV87_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5104214.1"
                     /translation="MANHLKTSCTHCQSVIKVNASHIGKRVRCPSCRQPFAVSTAESP
                     PPSAVDTGDHTPAAEVGTWDNAATISRSPPAESLHAPSAESQRVGPAPLAGLGRLGRF
                     ELRAELGRGAFGVVYRAYDPQLDRELALKVPTFDLKDTPKLARFQAEAKSAAKLRHPN
                     IVPTYESGVIDGRPYIATQFIAGQTLADRLQQGPPDLRQAAEWVRQLASALAYAHSQQ
                     IIHRDIKPANIMLDERGVPQIMDFGLAKRLHDDATMTTDGSVLGTPAYMSPEQARGEQ
                     AAIGPASDQYSLGVVLYELLTGEKPYAGSAHAVLAQVLGAEPLSPRHKRRSIPLDLNA
                     ICEKAISKSPGSRYASAGNFANDLSRWLEGLETHARPTTSLDRAFRWTRRNPLTAGLI
                     ASVVVAVIGGAAFSTWFAINANQWAAAEATQRQRADKETERAGKATAKSKADTLRAVE
                     SGAQAKIEAERAKLAAADAKRESERANQATAAVVAESERAFRAFYAEQLHLVQRDAES
                     GAFRAAVKRLQATMPEHTGGVDLRGFEWHYWNRVCRILDQSITFGKPVHHFLSGFTCL
                     QWKDERTLFVGYGLFQELAYDTQQVHSGMVPGYVVALSPDGSILMSRQGLSLPVTIHD
                     TRNHGAALPALRGPQRIHRAAISHDNARIATSSEDDALIRVWDVATGDLVSTLTGHSH
                     TAEAIAFDPTGKRLATGGGGIGGENALILWDVASGQQLPPLTGPPPGIQAIRFHPARP
                     HLAAAGADGTVCVWNVDDGQRLFDWTYQPSTPGAERAIVDLAYSRDGSRLAASAFDAI
                     AVLNAADGAVIREFEGSIGRVQPIAFAPDGSALLGGCCDGTLRIWSLTTQFAVTKIVH
                     TSGLHDIAFLPSSNAIAATCADGMVRVWDCSTGTITHTMQGLRGSSPVVAGGTMDSPV
                     IASPAGNRHISLWKADGTLRQGIDPGFDRCVHLSMDEKGSRLALGDNKQLAVFDTESG
                     KKVLDVPL"
     gene            complement(2973..>3969)
                     /locus_tag="VFV87_10415"
     CDS             complement(2973..>3969)
                     /locus_tag="VFV87_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5104215.1"
                     /translation="TAADPAYRLATERAARVEATMTCDVRADKLQASEWLVFVPVLPT
                     TAGQTDSSTKMTPAGKETAELSDLARPLLLARVRADNDELKHAAKMEVVYSATLWSRH
                     LVPLTRGAKAPPTNDLSAAEREAALRSAGDLSYDSDELKTWQREHKLLRDQGESEIDF
                     ARRVFATIRSEFTYAYQPEMDRAPAAVCKAGKSDCGGLSNLLVATLRSQGIPARTLAG
                     RWAVSADPNEKLNGHPYYQYHVKAEFFAAGVGWVPADMASGILHDRSREGLRYFGHDP
                     GDFLTQHIDGNLEIDTIHFGKQQLAVLQRPAFWVTGSGSLDDVKLLESWVVTMER"
BASE COUNT          688 a         1222 c         1296 g          763 t
ORIGIN      
        1 gaagaggaac atccaggact tttttgcccg attccgtgtc gaataccgcg agctgcttgt
       61 tgtctcccag cgccaaccgc gagcctttct catccatcga caaatgcaca cagcgatcga
      121 accctggatc aattccctgt cgcagtgttc catccgcctt ccatagggag atatgtcgat
      181 tgcctgccgg gctggcgatg accggcgagt ccatggtccc gcccgcgacg acgggcgaag
      241 agccgcgaag cccctgcatc gtgtgggtga tcgttcccgt cgagcagtcc cagacgcgca
      301 ccatgccgtc ggcacacgtg gcggcaatgg cattgctcga gggaaggaag gcgatatcgt
      361 gcagcccgct ggtatgcacg atcttggtga cggcgaactg cgtcgtgaga ctccagattc
      421 gcagcgttcc gtcgcagcat ccgcccagga gagctgaacc gtccggagcg aaagcaatcg
      481 gctgtacgcg gccaatgctc ccctcgaatt cgcgaatcac tgccccatcg gccgcattca
      541 acacggcaat tgcgtcgaat gcagaggcgg ctaggcgcga cccatcgcga ctgtaggcga
      601 ggtcaacgat ggcccgctcg gccccaggag tgctcggttg ataagtccaa tcaaacaggc
      661 gctgcccatc atccacattc caaacgcaca ctgtcccatc ggcgccggcg gctgcgagat
      721 gaggtctcgc aggatggaat ctgatcgcct gaatcccggg cggcggaccg gtcagcggag
      781 gcagttgctg cccggaagcg acgtcccaga gaatcagggc gttttctccg ccgatgccgc
      841 cgccgccggt tgccagccgc ttgcctgtgg gatcgaaggc aatggcttcc gcggtgtggc
      901 tgtgccccgt cagggtgctg accaggtcgc cggttgccac atcccaaacg cgaatcagcg
      961 cgtcgtcctc gctggaggtc gcgattctcg cgttgtcgtg gctaatcgct gcccggtgaa
     1021 tgcgctgcgg tccacgcagc gcggggagcg ccgcgccatg gttgcgcgtg tcatggatcg
     1081 ttaccggcag agagagaccc tggcgcgaca tcaaaatgct gccgtcgggg ctgagcgcca
     1141 cgacataacc agggaccatt ccgctgtgta cttgttgtgt gtcgtatgcc agttcttgaa
     1201 ataacccata gccgacaaac agtgtgcgtt catccttcca ttgcaggcag gtgaagcctg
     1261 acaagaagtg atgcacgggt ttgccgaagg taatcgactg atcgaggatt ctgcagacgc
     1321 gattccaata atgccattca aatccacgca ggtcgacgcc gccggtgtgc tcgggcatcg
     1381 tcgcctggag tcgcttgacg gccgcgcgaa acgcgcccga ttctgcatcc cgctgcacga
     1441 ggtgcagttg ttcggcatag aaagcccgaa acgcccgttc cgattccgcc acgaccgccg
     1501 cggtggcttg attggcccgc tcggactctc gtttggcatc cgcggctgcg agtttcgccc
     1561 gctccgcctc gattttcgcc tgcgcacccg attccacggc gcgaagggtg tcggctttcg
     1621 acttggcggt tgccttgccg gcccgctcgg tttccttgtc cgccctttga cgctgggtgg
     1681 cctcggccgc cgcccactga ttggcattga tggcaaacca cgttgagaac gctgcgccgc
     1741 cgatcacggc gacaacgacc gatgcaatca aacctgcagt cagaggattg cggcgcgtcc
     1801 agcggaacgc gcggtccagg gaggtagtgg gacgagcatg ggtttccagg ccctcgagcc
     1861 agcgggacag atcgttggcg aaattccctg ctgacgcata gcgactgcca gggcttttgc
     1921 tgatcgcctt ttcacaaatc gcattcaggt cgagcggaat gctacgccgc ttgtggcgcg
     1981 gcgatagtgg ctctgcgcca agcacctgcg caaggactgc gtgtgcagag cctgcatagg
     2041 gtttttcccc ggtcagcaac tcatacagca cgacgcccag gctgtactgg tcgctggcgg
     2101 ggccgatcgc ggcttgctcg ccgcgggcct gttcggggga catgtaagcc ggcgtaccca
     2161 ggactgagcc gtcggtggtc atcgtggcgt cgtcgtggag gcgcttggcc aggccgaagt
     2221 ccatgatctg cggcacgccg cgctcatcga gcatgatgtt ggccggcttg atgtcgcggt
     2281 gaataatctg ttgcgaatgg gcataggcca aggcgctcgc cagttggcgg acccattccg
     2341 cggcttggcg caggtccggc gggccttgct ggaggcggtc ggccagcgtc tgtccggcga
     2401 tgaactgcgt agcgatatac ggccggccgt cgatcacgcc gctctcgtag gttggcacga
     2461 tattgggatg gcggagcttc gcagcggatt tggcttcggc ctgaaagcga gccaacttcg
     2521 gcgtgtcttt caggtcgaag gtgggaacct tgagcgccag ttccctgtcc agttgcggat
     2581 cgtaggcccg ataaacgacg ccaaacgcgc cgcgacccag ttccgcccgc aattcgaacc
     2641 ggcccaatcg accaagcccg gccagcggcg ctgggccgac acgctgtgat tctgccgatg
     2701 gggcgtgcaa tgattctgcc ggtgggctgc gcgaaatcgt ggcggcgttg tcccaggtac
     2761 ccacctcagc cgcaggcgtg tgatcgcccg tgtccacggc gctcggtggc ggcgattcgg
     2821 ccgtggagac ggcaaatggc tgccggcagg acgggcagcg cacccgctta ccgatgtggg
     2881 acgcattgac cttgatgacc gactggcagt gtgtgcagga ggttttcaga tggttcgcca
     2941 aggcggtaag tccggctgtg tttgcctgat cgttatcgct ccatcgtaac gacccacgat
     3001 tccaatagct tgacgtcgtc gagcgagccg ctgccggtga cccagaaggc ggggcgctgg
     3061 aggacggcga gctgttgctt gccgaagtga attgtgtcga tttccaggtt gccatcgatg
     3121 tgctgcgtca ggaaatcgcc cgggtcgtgg ccgaaataac gcagcccctc gcgagaacgg
     3181 tcgtgcagaa tgccggaggc catgtccgcg ggaacccagc ccacacccgc cgcaaagaat
     3241 tcggctttga cgtgatattg atagtatggg tggccgttga gcttctcgtt cggatcggcc
     3301 gaaacggccc agcggccggc gagcgtgcgt gcgggaatgc cctgcgagcg gagcgtggcg
     3361 acgagcaggt tcgagagccc gccgcaatcg gacttgccag ccttgcacac tgcggcgggg
     3421 gcgcggtcca tttccggctg gtaggcgtat gtgaattcgc tgcggatggt cgcaaacacg
     3481 cggcgggcga agtcgatctc ggactcgccc tggtctcgca gcagcttgtg ctcgcgttgc
     3541 caggtcttca attcgtcgct gtcatagctc agatcaccgg cagatcgcag ggccgcctcg
     3601 cgctcggcag cggagagatc attggtcggc ggagccttgg ctccgcgagt tagcggcaca
     3661 agatgacgcg accacagtgt cgcagaataa acgacctcca tcttcgcggc atgcttcagt
     3721 tcgtcgttat ccgcacgcac gcgggccagc agcagcggcc gcgccaggtc ggacaactcg
     3781 gcggtttcct tgccggcggg tgtcattttg gtgctggagt cggtctgccc ggcggtcgtc
     3841 ggcagtacgg gcacaaatac gagccactcg ctggcctgga gcttgtctgc gcggacgtcg
     3901 caggtcatcg tcgcctcgac ccgcgccgcg cgctcggtcg ccagccggta agcggggtca
     3961 gccgccgtg
//
LOCUS       DASUGB010000542         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1034568, whole genome shotgun sequence.
ACCESSION   DASUGB010000542 DASUGB010000000
VERSION     DASUGB010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034568"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFV87_10420"
     CDS             <1..462
                     /locus_tag="VFV87_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104216.1"
                     /translation="PMAEIAITEISLEREATANKDGSVLGTLTVGTTSFPTIERGAGY
                     VNLKTGSYVMKHSIKNTGRRIQCLRPEIVQIYTLLIHDALGDSSNNLEGCVAPGMEKK
                     ANNMGIRRSAEAMEAVWKLLGGFEEGKKVTLNVLNNVPGETRTKDTWERLK"
     gene            522..1016
                     /locus_tag="VFV87_10425"
     CDS             522..1016
                     /locus_tag="VFV87_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104217.1"
                     /translation="MPGLIKQVKIVGTPQQVNKPTRFGPTIGHQFVVEILAEDIGNCR
                     LQWWEKTERPYTPGMLPGVWCDLFALHPQSDVFAPWRGHVSMGPAEIQLHDPPGIMVS
                     DCQFRELCFEIRVREYNVVLGTNNQRRIRARQVLRSDPLSLEFHIMENDFFSNAEDGA
                     PTWG"
     gene            complement(1079..2107)
                     /locus_tag="VFV87_10430"
     CDS             complement(1079..2107)
                     /locus_tag="VFV87_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104218.1"
                     /translation="MKLRVGIVGLGAAWESRHRPALRALSDRFEVRAVCCEVAHLASQ
                     AARDLGAAEVDGFRALCGRDDVDVVLILSPEWYGPLPVLAACDFGKSVYCGAALDIED
                     PEQAQLLKRRVGEAGIAFMAEFARRLAPATLRLKELIATRLGKPKLLFCHRRSSIEPG
                     GSPRRPTRHCPTMQREMIEQIDWCRYVAALEPTSVVGIEHCCTETSDGDDYQMMSLDF
                     SPAGQLGTGPIAQISCGRYMPVAWPEAVAFRPPAALQVCCERGVAFIDLPSTLIWFDE
                     AGRHMEALDSERPVGERLMMQFYRAVTSLVRKTSDMDDAYRALSIALAARKSFETGQR
                     IQISAEPA"
     gene            2250..>2435
                     /locus_tag="VFV87_10435"
     CDS             2250..>2435
                     /locus_tag="VFV87_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104219.1"
                     /translation="MNPPTRVVFYQQGFLILTPLIICPSFTTRAKETIMLGPAVAFVL
                     QAATLVSGAQTYEQAYED"
BASE COUNT          465 a          736 c          836 g          398 t
ORIGIN      
        1 cccatggccg aaatcgcgat caccgaaatc tcgcttgaac gggaagcgac cgcgaacaag
       61 gacggcagcg tgctgggcac gcttaccgtg ggcacgacgt cgttcccgac cattgagcgg
      121 ggcgcggggt acgtcaacct caagaccggc agctacgtga tgaagcactc gatcaagaac
      181 acgggccgcc gaatccagtg cctgcggccg gagatcgttc agatttacac gctgctgatc
      241 cacgacgccc tgggcgacag cagcaacaac ttggaggggt gcgtggcgcc gggcatggag
      301 aagaaggcga acaacatggg catccgccgg tcggccgaag cgatggaggc ggtctggaag
      361 ctcctcggtg gatttgagga aggcaaaaaa gtgacgctca acgtgctgaa caacgtgccg
      421 ggcgaaaccc gcaccaagga tacgtgggag cggctcaagt agcgggcagt gcaccttcaa
      481 tcgcccgccg ggcctgcaca catcgactgg agactcaaga catgcccggg ttgatcaagc
      541 aggtgaaaat cgtcggcacg ccgcagcagg tgaacaagcc gacgcgcttt ggcccgacga
      601 tcggccacca gttcgtcgtg gaaatcctgg ccgaagacat cggcaactgc cgcctccagt
      661 ggtgggaaaa gaccgagcgc ccgtacacgc cggggatgct gccgggcgtg tggtgcgacc
      721 tgttcgccct gcacccgcaa tccgacgtgt tcgcgccgtg gcgggggcac gtcagcatgg
      781 gtccggccga gattcagctg cacgatccgc ccgggatcat ggtgagcgac tgccagttcc
      841 gcgagctgtg ctttgaaatc cgcgtgcggg agtacaacgt ggtgctgggc accaacaacc
      901 agcggcggat ccgcgcccgg caggtgctcc gcagtgaccc gctgtcgctc gagtttcaca
      961 tcatggaaaa cgactttttc agcaatgccg aggacggggc gccgacctgg ggttaggcgg
     1021 acggcacaca gcccgcgatg tacggcctgc cgctggtcgc tcatgggcct ggcaggcgct
     1081 aggccggctc ggcggaaatc tggatgcgct ggcccgtttc aaaacttttg cgggcggcca
     1141 gggcgatcga cagggcgcgg taggcgtcgt ccatgtcgct ggtcttgcgc accaggctgg
     1201 tgacggcccg gtagaactgc atcatcagcc gctcgcccac ggggcgttcg ctgtcgaggg
     1261 cttccatgtg gcggcccgct tcgtcgaacc agatgagcgt ggaggggagg tcgatgaagg
     1321 ccacgccccg ctcgcagcag acttgcaggg cggcgggtgg gcggaaggcg accgcttcgg
     1381 gccaagcgac gggcatgtag cggccgcagc tgatctgggc gattggcccc gtgccgagtt
     1441 gcccggcggg cgagaagtcg aggctcatca tctggtagtc gtcgccgtcg ctggtttcgg
     1501 tgcagcaatg ctcgatgccg acgacggacg tgggttccag ggccgcgacg tagcggcacc
     1561 agtcgatctg ctcgatcatt tcgcgctgca tggtgggaca gtggcgggtt ggccggcgcg
     1621 gcgagccgcc gggctcgatc gacgagcggc ggtggcagaa caagagcttg ggcttgccca
     1681 agcgggtggc gatgagctcc ttcaagcgga gggtcgcggg ggccaggcgg cgggcgaact
     1741 cggccatgaa ggcgatgccc gcctctccca cgcggcgctt gagcagctgg gcctgctcgg
     1801 ggtcttcgat atcgagggcc gccccgcaat agacgctttt gccgaagtcg caggccgcca
     1861 agaccggcag cgggccgtac cactcgggag agagaatgag aacgacatcc acgtcgtcgc
     1921 ggccgcacag ggcgcggaag ccatccactt cggccgctcc caaatcgcgg gcggcctggc
     1981 tggccaggtg ggcgacttca cagcaaacgg cgcggacctc gaagcggtcg gacaaggccc
     2041 gcaatgccgg ccgatggcgc gactcccagg ccgcccccag tccgacgatg cctacgcgta
     2101 atttcatgct gccccagcga gaagtagctc tcacagccgg caatcgttgc tggccgcagg
     2161 catgggggga ttatacgcgt cgcagctggt tttgtcgtcc tgacagtccg aaagtgcgga
     2221 gcggataatt tgcaggattc cgcaactcag tgaatccacc cacaagagtg gttttctatc
     2281 agcaaggctt tcttatactc acccccttaa tcatttgccc cagtttcaca actcgtgcca
     2341 aggagacgat catgttgggg cctgctgtag cattcgtgtt gcaggcggcg acgctggtaa
     2401 gtggagcgca gacttacgag caagcctacg aagat
//
LOCUS       DASUGB010000543         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2007342, whole genome shotgun sequence.
ACCESSION   DASUGB010000543 DASUGB010000000
VERSION     DASUGB010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007342"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFV87_10440"
     CDS             <1..470
                     /locus_tag="VFV87_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104220.1"
                     /translation="PIIAGDLVIGTCGFVTAQKHFVAVRPTGDGKAKEVWRIEKAVAY
                     MPTPIVKSNRIFSCTEKGIFSCIDAKSGKVIWQERLDNEFSASPVCAGNAIYCTANDG
                     QVYVVAAADSFKRLGRCQLPGATQSTPAIAADCMTFRAGGRIVAVPRAPVGSR"
     gene            867..1550
                     /locus_tag="VFV87_10445"
     CDS             867..1550
                     /locus_tag="VFV87_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5104221.1"
                     /translation="MIATLHAQPSSNILLDNISWETYETLLRDLEGERRFRITYDRGS
                     LEIMTLSHEHEFSDELLNRYVHMLTFELDIPIHSGGSTTLRRRKRAKGLEPDKCYWIQ
                     NELLMRGKKMFDIEVDPPPDLAIEMEVTRSALNRMGIYAAIRVPEVWCYDGESLRVHV
                     LGENGKYKESAKSLAFPFLPMKELERFLSKAGAQDETTLLRSFCKWVRETIAPALQTK
                     SKNGRKSVK"
     gene            1620..>2018
                     /locus_tag="VFV87_10450"
     CDS             1620..>2018
                     /locus_tag="VFV87_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104222.1"
                     /translation="MKWTLVLSACLACASLLAAQGTKPGNYRPTIDKGLAWLAAQQAK
                     DGSWSEKQGINPAANTASAGMALLADGNTLAEGKYRANVRLALNWFLALEPMNAKYGD
                     ALVDPEYPGSTGRYMMEHGLGMSFLAKLYGE"
BASE COUNT          422 a          615 c          624 g          357 t
ORIGIN      
        1 cgccgatcat cgccggcgat ctggtgatcg gcacgtgcgg gttcgtcacc gcccagaagc
       61 acttcgtcgc cgtgcgcccg accggcgatg gcaaggcgaa agaggtgtgg cgcattgaaa
      121 aagcggtcgc gtacatgccg acgccgatcg tcaagagcaa ccgtatcttc agttgcaccg
      181 aaaaggggat attcagctgc atcgacgcga agtccggcaa ggtgatctgg caagaacgcc
      241 tggacaacga gttctccgcc tcgccggtgt gcgccggcaa tgccatctat tgcaccgcca
      301 atgacggcca ggtctacgtc gtcgcagcgg cggattcgtt caagcggctg ggccgctgcc
      361 aattgccggg cgcgacgcag agcacgccgg ccatcgcggc ggattgcatg acctttcggg
      421 cgggagggcg gatcgtggcg gtgccgcggg cgcccgttgg ttcgcggtga acgtgggaca
      481 agactgactt cctatcgccg gttccggggc gacggcgtcg cgggcttgag caacgacgac
      541 ttcaggagcg cgtcgcttgg ctccagcctc ggaaaaactg aagggcgcca aacgacagta
      601 accatggtca gcgagtcctc aatctgcaag tattctgcgt aaatgcgatt cgtttgaaac
      661 ccatcgcgga acccaccggt tgccagggag caggaatgcc gcgagccgtc tacggtaacg
      721 cccggcggcc aatccggtca acgaatttcc gagccaaacg gccgggcggg cgagcagacg
      781 aaggcccggc gttccggcgc ctttgcgctt tccggacatg gccgggattg ttattctgaa
      841 ttggagaatt gccttggagt catcacatga ttgccaccct gcacgcacag ccatcgtcca
      901 acattctcct ggacaacatc tcctgggaaa cctacgagac attgctgcgc gatctcgaag
      961 gcgagcgccg ttttcgcatt acctacgatc gagggagtct cgaaatcatg acgctgtcgc
     1021 acgagcatga attctccgat gaactgctaa accgttacgt tcacatgttg acgttcgaat
     1081 tggatatccc catccacagt ggcgggtcca ccacgctaag acgccgaaaa cgcgcgaagg
     1141 ggctcgaacc cgacaaatgc tactggattc aaaacgagct gctcatgcgc ggcaagaaga
     1201 tgttcgacat cgaggtcgat ccgccgcccg atctggcgat cgagatggaa gtcacccgca
     1261 gcgcgctcaa tcgcatggga atctatgcgg cgatccgcgt tcccgaggtt tggtgctatg
     1321 acggcgaatc attgcgggtt cacgttcttg gcgagaacgg caagtacaag gaaagcgcaa
     1381 agagcctggc gtttcccttt ctgcccatga aggaactgga gcgcttcctg agcaaggccg
     1441 gcgcgcaaga tgaaacgacg ctgctgcgtt cgttctgcaa gtgggtgcgc gagaccatcg
     1501 ccccggcgct gcagaccaag agcaagaacg gacggaaatc ggtcaagtga gccgtcagcg
     1561 tttcttcccc cggtcgtcct gtctttggcc acatcaccac ctcggctgga gggattgcca
     1621 tgaaatggac gctcgttctg agcgcgtgcc tggcgtgcgc atcgctgttg gccgctcaag
     1681 gcacgaagcc gggcaattac cgtccgacca tcgacaaggg cctggcctgg ctcgccgccc
     1741 agcaagccaa ggacggaagc tggtcggaaa agcaaggcat caaccccgcc gcgaacaccg
     1801 cttccgccgg catggcgctg ctggccgacg gcaacacgct cgccgagggc aaataccgcg
     1861 cgaacgtgcg gctagcgctc aactggttcc tcgcgctcga gccgatgaat gccaagtacg
     1921 gcgacgcgct cgtcgatccg gaatacccgg gcagcaccgg ccgctacatg atggagcacg
     1981 gcctcggcat gtcgttcctg gccaagctct acggcgag
//
LOCUS       DASUGB010000544         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1449644, whole genome shotgun sequence.
ACCESSION   DASUGB010000544 DASUGB010000000
VERSION     DASUGB010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449644"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2528)
                     /gene="dnaE"
                     /locus_tag="VFV87_10455"
     CDS             complement(<1..2528)
                     /gene="dnaE"
                     /locus_tag="VFV87_10455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5104223.1"
                     /translation="MRYCELHCKTNFSFLEGASHADELAQRAAELGYSALAVTDRNSL
                     AGVVRAHTAAKELGLKLLIGAEVHPEDAQPVILWATDRKSYGRLSRLLTVGRRRAEKG
                     QCRLTVEDIAAHAEGLIAGVMPAKDEGRRTKDENLLRTSHFVLRTFPRRCYLLAELFR
                     GPDDAAELERLIALSQQTGLPLVAAGDVHYHSPARQPLHEVLTATRLGTTVDRLFEHR
                     FSNAQRHLRPTSEIAAMFARVPEALARTQEIADQCIFSLDELRYEYPEELAREGFTPL
                     EFLTYLAWKGARKRYPSGIPAKVAQLIEHELKLIEELRYEAYFLTVWDLVRFARSRGI
                     LCQGRGSAANSAVCYCLGVTSVDPSRIEVLFERFVSKERNEAPDIDVDFEHERREEVL
                     QYIYDKYGRDRAGMTAVVITYRTRSAIRDVGKALGLSAGRVDAIAKQVEGRELDRAAK
                     VPPKSDKHEDPQPERGQGSGVRGQEEDASHPQSAIRNPKSPAPTATLARRVAETGLDP
                     LSPLGQRLIYLVTELVGFPRHLSQHVGGMVMTQGPLCELAPIENAAMPGRTVIEWDKN
                     DLDDLGILKVDCLALGMLTAIRKAFDLIHLHQPGANLLPENCELTSRAPRRNQVWSRL
                     RTEKLTLATVPAEDPKVYDMICRADTVGVFQIESRAQMSMLPRLQPREFYDLVIEVAI
                     VRPGPIQGNMVHPYLRRRNGEPVPPYPTKEIEAVLQKTLGVPLFQEQAMRLAVVAAGF
                     TPGEADQLRRAMGAWRKTGVIDQFRKKLVDGMLSRGLSQEFAEQVFNQIRGFGEYGFP
                     ESHAASFALLVYVSAWIKCYYPAAFAAAIINSQPMGFYAPAQLIR"
BASE COUNT          449 a          835 c          837 g          464 t
ORIGIN      
        1 cggatgagct gcgcgggagc ataaaagccc atcggctggc tgttgatgat cgcggcggcg
       61 aaggcggcgg ggtaatagca cttgatccag gccgagacat agacgagcag ggcgaaactc
      121 gccgcgtgcg actcgggaaa gccatactcc ccaaagccgc gtatctggtt gaagacctgc
      181 tcggcaaact cctgcgagag gccacggctg agcatgccat caaccagttt cttgcggaac
      241 tggtcgatca cgcccgtttt tcgccaggcc cccatcgccc ggcgaagctg gtcggcctcg
      301 cccggcgtaa agcccgcggc gaccaccgcc agccgcatcg cctgctcctg aaagagcggc
      361 acgccgagtg tcttttgcag gaccgcttca atctccttcg tcgggtatgg cggcaccggc
      421 tcgccgttgc gccgccgcag atacgggtgc accatattcc cctgaatcgg ccccgggcgg
      481 acgatcgcca cttcaatcac caggtcgtag aactcgcggg gctgcagccg cgggagcatg
      541 ctcatctgcg cccggctttc gatctgaaag acgccgaccg tatcggcccg gcagatcatg
      601 tcatagacct tggggtcttc ggcggggacg gtggcgagcg tgagcttctc tgttcttagc
      661 cgggaccaaa cctggttccg gcgtggcgcg cgcgatgtca actcgcagtt ctccgggagc
      721 aagtttgctc ccggctgatg gagatggatc aaatcgaacg ccttccgaat cgccgtcagc
      781 atgccgagcg cgaggcaatc gaccttgagg atgccgaggt cgtcgaggtc gttcttgtcc
      841 cactcgatga ccgtgcggcc gggcatggcg gcgttttcga tcggcgccag ctcgcagagc
      901 ggcccctgcg tcatcaccat tccacccacg tgctgggaca aatgccgcgg aaagccgact
      961 agctctgtca ccaaataaat caggcgctgg ccgaggggcg agagcggatc gaggccggtt
     1021 tcggccacgc ggcgggcgag ggtcgccgtc ggcgccggag atttcggatt gcggattgcg
     1081 gattgcggat gggaggcatc ttcctcctga cccctgaccc ctgacccctg gccccgctcc
     1141 ggctgcggat cttcatgctt gtccgacttt ggcggcacct tagcggcgcg gtccagctcg
     1201 cggccttcga cttgcttggc aatcgcatcg acacgacccg ccgacaggcc gagggctttg
     1261 ccgacgtcgc ggatcgcgct ccgcgtgcga tacgtgatga cgacggcggt catgccggcc
     1321 cggtcgcggc catatttgtc gtagatatat tgcagcacct cctcgcgccg ctcgtgctcg
     1381 aaatcgacgt cgatatccgg ggcctcgttg cgctccttgc tcacgaaccg ctcgaagagg
     1441 acctcgatgc ggctgggatc gacgctggtg acgcccaggc aatagcagac ggcggaattt
     1501 gccgccgagc cgcggccctg gcagaggatg ccccgcgagc gggcgaagcg gacgaggtcc
     1561 cagacggtga gaaaataggc ctcgtaccgc agctcttcga tcagcttcag ctcgtgctcg
     1621 atgagctggg caaccttcgc ggggatgccg ctgggatagc gtttgcgggc ccctttccag
     1681 gcgaggtacg tgagaaactc cagcggcgtg aagccttcgc gggccagttc ctcaggatat
     1741 tcgtaccgca gctcatcgag cgagaagatg cattggtcgg ctatttcctg cgtccgcgcg
     1801 agggcctccg gcacgcgggc gaacatcgcg gcgatctcac tggtcggccg cagatgccgc
     1861 tgggcgttgg aaaaccggtg ctcgaagagg cgatcgaccg tcgtgcccag gcgcgtcgcc
     1921 gtcagcacct catgcagcgg ctggcgggcc ggcgagtggt aatgcacatc ccccgctgcc
     1981 accagcggca ggccggtttg ctgcgaaagc gcgatgagcc gctccagttc cgccgcatca
     2041 tccggcccgc gaaacagctc agcgaggagg tagcaacggc gggggaaagt acgaagtacg
     2101 aagtgcgaag tacgaagcag attttcgtcc ttcgtccttc gtccttcgtc cttggccggc
     2161 attacgccgg caatcagtcc ttccgcgtgt gccgcaatat cttccaccgt cagccggcat
     2221 tggcctttct cggcccggcg acggccgacg gtcagcagcc gcgacagccg gccgtaggac
     2281 ttgcggtcgg tggcccagag gatgaccggc tgggcatctt cgggatgcac ctccgccccg
     2341 atcagcagct tgaggccgag ttcctttgcc gccgtatgcg cccgcacgac gccggccagc
     2401 gaattgcgat cggtcacagc cagcgcggaa tagcccaact cggccgcgcg ctgcgccagt
     2461 tcatcggcgt gcgaagcgcc ttcgagaaag gagaagttcg ttttgcagtg cagctcgcag
     2521 tagcgcatcc gcgaggcctc cgcattacga gaaatcgccg tgcaagtgcc actggccggt
     2581 tttca
//
LOCUS       DASUGB010000545         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1449678, whole genome shotgun sequence.
ACCESSION   DASUGB010000545 DASUGB010000000
VERSION     DASUGB010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449678"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFV87_10460"
     CDS             complement(<1..181)
                     /locus_tag="VFV87_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX5104224.1"
                     /translation="MSSIRVLVGTKKGGFVLSSDAGRKKWSVSGPFFNGWEVYHLKGS
                     PVNPDRIYCSQTSGWF"
     gene            973..1422
                     /locus_tag="VFV87_10465"
     CDS             973..1422
                     /locus_tag="VFV87_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5104225.1"
                     /translation="MNKTQFLRQTEQRLLARRGALKAALAGDQRMLQTLHESGVGDEI
                     DAAIASQQAELQSQMAEVESRELVQIDQALAKIRAGQYGRCETCERQIAPLRLKYLPY
                     AADCIACARREERHDTAQPLSRPVNRIAAFIDDEQEPAVAEAELELR"
     gene            1657..2193
                     /locus_tag="VFV87_10470"
     CDS             1657..2193
                     /locus_tag="VFV87_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104226.1"
                     /translation="MRCALSLLVAILFPLGLAAEEPAKPAAPTQAELIKKLAKDLTGA
                     RLVGKFTVTGKGEVTPKDEEYTITSALKLDEPDLWLLKARVKYGKTDGVFPIPLEIKW
                     AGDTPVITLTDLEIPGLGTFSSRVVIYEGRYAGTWQHGDVGGHLFGVLKPATDDKPAA
                     DKPVPARPASAKPAADKP"
     gene            2277..>3020
                     /locus_tag="VFV87_10475"
     CDS             2277..>3020
                     /locus_tag="VFV87_10475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX5104227.1"
                     /translation="MSAAPSPIHPAPAHPAVPQPSQVVIYQTGVLTRIWTWLGWTGFA
                     ICSVLVLGQWIALYDYFDDSHGITEKYVQGAEYGSDKVAVLSIEGVIMEGDGFVKQQI
                     DRIREDDDVKAVVVRVDSPGGTVTGSDYIYHHLSKLRQEKNIPVVVSMGSIAASGGYY
                     VSMAVGDQPDSIYAEPTTTTGSIGVIIPHYDLSGLLDRFDVKDDSIASHERKQMLSMT
                     RPMPPEHREIIQKHINAMFERFKSIVKDGR"
BASE COUNT          612 a          958 c          975 g          475 t
ORIGIN      
        1 cgaaccagcc actggtttgc gagcaataga tgcggtcggg attcaccggg gagcctttca
       61 ggtgatagac ctcccagcca ttgaagaagg gaccgctgac ggaccatttt ttccggccgg
      121 cgtcggagga gaggacgaag ccgcctttct tggtgccgac gagaacgcga atagaactca
      181 tggatcgttc cttaagagtg gtggctctga atagcttggt tgccccgaaa tgggcaacga
      241 ctggcagcca tgataatgga tgcagcggcg gaacaatggc cgccggtctt ggtgaatcct
      301 cggccgcacg cttcagctcg tgaatctcaa ttctgcccac ttgcagcatg acgtggcgac
      361 gattagccac gcgagtggtg ccggcctggc gccggctgcg aagccgcaag ataagcggcc
      421 cagcccggca gggcgagccg attcgccgcg ctggtcaaaa aatcgttcca tcgtggcgat
      481 gcggcgccgc tgcgaacgag ccgccgagcg ctttgaccga ttgcggcagg gcgcaaaggg
      541 cggcggcaat tccggcgggc ggcaagatgg ccggcagccc agcgaactga cttgattgca
      601 gccagcagtc ggcggattcg gcggggcaaa acgcctcgaa aaaggcagca aaacaggggc
      661 gactcggagg atagccgcca gcgatttgcc aggcgacagg acggcgggcc ggtgcgtcaa
      721 ttgctcctag cgcaccagtc gtacaacccg catcgttttt cagaacaacc ccctgaatcc
      781 tgccgtatgg aacgtgggtt gcattacccc aagcagcccg gcggagtcgg ccaagacggc
      841 acggacgcct gcgtgaaatg agaagtgagc aggtagccgg ccgttaagac ggccaaggag
      901 aatagaccac aagatcggaa gatcgcggac cggtgcggct ctggccgccg cggttccgag
      961 ggaggcacaa tcatgaacaa gacccaattc ctgcgtcaga cggaacagcg tttattggcc
     1021 cgccggggcg cgctcaaggc ggccctggcc ggcgaccagc gaatgctgca aacgctccac
     1081 gagtcgggcg tcggcgacga aatcgacgcc gcgatcgcct cgcagcaggc cgagctccag
     1141 tcgcaaatgg ccgaagtcga aagccgcgaa ttggtgcaga tcgaccaggc cctggccaag
     1201 atccgcgccg gccaatatgg ccgctgcgaa acgtgcgagc ggcagatcgc tcccttgcgg
     1261 cttaagtacc tgccctacgc ggccgattgc atcgcctgtg cccgccgcga agagcggcac
     1321 gacacggccc agccgctgtc gcggccagtc aaccggatcg cggcgttcat cgacgacgag
     1381 caggagcccg ccgtggccga ggccgagctg gagctgcgat aggcgtcgct ggatcgccca
     1441 gatgccgagg tgactcgacg aggctgcaca gccaagggcg gcgcgaaaac ccgcgccgcc
     1501 ctgccttttg cgccgcaggc ggtataattg ctgccagccg ggaccaaact ggtcccggag
     1561 cgccgctggc agagctgctt ggccgcgctc cggaggcgcg tttgcctccg gctaaggggt
     1621 cgttatccgg gagcgtcgat atccgggagc taagtcatgc gatgtgcgct gtccctgctg
     1681 gtggcgatct tgttccccct ggggctggcg gccgaggagc cggcgaaacc ggcggcgcca
     1741 acgcaagcgg agttgatcaa gaagctcgcc aaagacctga ccggcgccag gctcgtcggc
     1801 aaattcaccg tcaccggcaa aggagaggtc acgcccaagg acgaggagta cacgatcaca
     1861 agcgccctga agctcgacga gcccgatctg tggctgctca aggctcgggt caaatacggc
     1921 aagacggacg gcgtcttccc gatcccgctc gaaatcaagt gggccggcga tacgcccgtg
     1981 atcacgctca ccgacctcga aatccccggt ctgggcacat tcagctcgag agtggttatt
     2041 tatgaaggcc ggtatgccgg aacatggcag cacggcgacg tgggcggcca cctcttcggc
     2101 gtgctcaagc ccgctacgga cgataagcct gctgcggaca agcctgttcc tgccaggcct
     2161 gcttctgcca agcccgccgc cgacaagccc tgatcgttgc ccgcccagcg gctaaccgcg
     2221 ccgtacaata gcggcagggc cggtgttccc ggcccgcttt ttctggagac ccgctgatga
     2281 gcgccgcgcc ctcgcccatc caccccgcac cggcccaccc ggctgtcccc cagccctcgc
     2341 aagtcgtcat ctaccagacc ggcgtactga cccgcatctg gacgtggctc ggctggaccg
     2401 gctttgcgat ttgcagcgtc ctggtgctgg ggcagtggat cgccctgtac gactatttcg
     2461 acgattctca cggcatcacc gaaaagtacg tgcaaggagc cgaatacggc agcgacaaag
     2521 tcgctgtcct gtcgatcgaa ggggtcatca tggaagggga cggcttcgtc aagcagcaga
     2581 tcgaccgcat tcgcgaggac gacgacgtga aggcggtcgt cgtgcgggtc gattcccccg
     2641 gcggcaccgt caccggctcg gactacatct accaccacct ctcgaagctg cggcaggaga
     2701 aaaacatccc cgtcgtcgtg agcatgggga gcatcgcggc cagcggaggc tactacgtct
     2761 cgatggccgt gggcgaccag ccggactcaa tctatgccga gccgacgacg acgaccggct
     2821 cgatcggcgt gatcattccg cactacgacc tgagcggcct gttggaccgc ttcgacgtga
     2881 aggacgactc gatcgccagc cacgagcgga aacagatgct gagcatgacg cggcccatgc
     2941 ctcccgagca ccgcgagatc attcagaagc acatcaatgc gatgttcgag cggttcaagt
     3001 cgatcgtcaa ggacgggcgg
//
LOCUS       DASUGB010000546         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1591246, whole genome shotgun sequence.
ACCESSION   DASUGB010000546 DASUGB010000000
VERSION     DASUGB010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591246"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFV87_10480"
     CDS             <1..246
                     /locus_tag="VFV87_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104228.1"
                     /translation="KPAGEWNQLKILITPEKCATWVNGKLYYEYVKGSEDWNARVAKS
                     KFAKMENFGKPTSGFICLQDHGNPVKFRNIKIRSIEK"
     gene            complement(282..743)
                     /gene="tadA"
                     /locus_tag="VFV87_10485"
     CDS             complement(282..743)
                     /gene="tadA"
                     /locus_tag="VFV87_10485"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5104229.1"
                     /translation="MTHEHYMRLALEQAQAALAEDEVPIGAVIAQGERVIAAAHNQRE
                     TLSDPTAHAEIIAITQAAAALEDWRLDGCTLYVTLEPCIMCAGAIVLARIPMVVYGAT
                     DPKAGAVQSLYHLLNDERLNHRCQVVPGVLAAPCGELLTRFFQEQRRLGKK"
     gene            complement(830..1876)
                     /locus_tag="VFV87_10490"
     CDS             complement(830..1876)
                     /locus_tag="VFV87_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104230.1"
                     /translation="MKLRVGIVGLGEAWESRHRTALRALSDRFEVRAVCCEVAHLAEH
                     AARDFHAIEVDGFRALCARDDVDVVLILAPEWYGPLPVLAACDYGKSVYCGAMLDIDD
                     PSQATQLKSRVEQAGVAFMAEFPRRLAPATLRLKELIATRLGRPKLLFCHRRSTIEPG
                     GAPRRGRYCPTMLREMIEQVDWCRYVVGTEPTSVLGIEHGCYDSADGDDYRMMSLDFS
                     EPGKMGEGPIAQISCGRYMPTSWPEAVSFRPPAALQVCCERGVAFIDLPATLIWFDEY
                     GRHMESLDSERPIGERLLMQFYRAVTSLVRRTNDLEDAYRALSVVLAARESFDNGRRI
                     FLPDNPEATAKPSA"
     gene            2261..2698
                     /locus_tag="VFV87_10495"
     CDS             2261..2698
                     /locus_tag="VFV87_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5104231.1"
                     /translation="MSAFSAGIVVHIALLAGGAQPYEQAQKQAAANGQPLLVLVGTDW
                     CPGCRTMKDGVLARMSAGGRLAKVNYTMVNADRESDLAAHLIRGNAIPQLIVFSKRPD
                     GEWHREQITGTASETQVAALIERARAAQAAKTARPTSAAVGGN"
BASE COUNT          529 a          821 c          886 g          490 t
ORIGIN      
        1 aagccggccg gcgagtggaa ccagctcaag atcctgatca cgcccgagaa gtgcgcgacg
       61 tgggtcaacg gcaagctgta ctacgagtac gtgaagggtt cggaggactg gaacgcccgc
      121 gtcgccaaga gcaagttcgc caagatggag aacttcggca agccgacgag cggattcatc
      181 tgcctgcagg accacggcaa tccggtgaaa ttccggaata tcaagattcg ctcgatcgag
      241 aaataaggcg cgccgtggga cgggtttcca acccgtctct gctacttctt ccccagccgc
      301 cgctgctcct gaaagaaccg cgtcaatagc tcgccgcaag gggcggcgag cacgccgggg
      361 acgacctggc agcggtggtt gaggcgctcg tcgtttagca ggtgatagag cgattgcacg
      421 gcgccggcct tggggtcggt cgcgccatag acgaccatcg gaatgcgggc caggacgatt
      481 gccccggcgc acatgatgca cggctccagc gtgacataga gcgtacaccc gtccagccgc
      541 cagtcttcca gggcggcagc cgcctgcgta atggcgatga tttcggcatg ggcggtcgga
      601 tcgctcagcg tctcgcgctg attgtgggcc gccgcgatga cccgctctcc ctgcgcgatc
      661 acggccccaa tcggcacctc gtcctcggcc aaagccgctt gcgcctgctc cagcgccagc
      721 cgcatgtaat gctcgtgggt catgttctac tgggcagact ccagcggaag tgcatgtgtg
      781 ccggcaggta aatcgaaaca gccagccgct gctcacaagg cagcgcggct taggcggatg
      841 gcttggcggt tgcttccggg ttgtctggga ggaaaatgcg gcggccgttg tcgaagcttt
      901 cgcgggcggc gaggacgact gagagggccc ggtaggcatc ttccaagtcg ttggtccggc
      961 ggacaaggct ggtgacggcc cgatagaact gcatcagcag ccgctcgccg atcggccgtt
     1021 cgctgtcgag cgattccatg tgccggccgt attcgtcgaa ccagatgagg gtcgccggca
     1081 gatcgatgaa cgccacgccc cgctcgcagc acacctgcaa ggctgcgggt gggcggaacg
     1141 aaacggcttc gggccaggaa gtgggcatgt aacggccgca actgatctgg gcgatcggtc
     1201 cttcgcccat ttttccgggc tccgaaaaat cgaggctcat catgcggtag tcgtcgccgt
     1261 cagccgagtc gtagcatccg tgctcgatgc cgaggacgct ggtgggttcg gttccaacga
     1321 catagcggca ccaatcgacc tgttcgatca tctcgcggag cattgtcgga caatagcgac
     1381 cgcggcgcgg cgcgccgccg ggctcgatcg tcgagcggcg atggcagaac aggagcttgg
     1441 gccggcccag gcgcgtggcg atcagttcct tgaggcggag cgtagccggg gccaggcggc
     1501 gggggaattc ggccatgaac gcgacgccgg cttgttcgac gcgggacttg agctgcgtcg
     1561 cctggctggg gtcatcgatg tcgagcatcg cgccgcaata cacgctcttg ccgtagtcgc
     1621 acgcggccag cacgggcagc gggccgtacc attcgggggc gagaatcagg acgacatcga
     1681 catcgtcccg cgcacaaagg gcgcggaagc catcgacctc gatggcgtgg aaatcgcggg
     1741 cagcgtgctc cgccaggtga gcaacctcgc aacagaccgc gcggacctcg aatcgatcgg
     1801 acagggcccg cagcgcagtg cggtgccgcg actcccaggc ttccccaagg cccacgatgc
     1861 caacacgaag tttcatggag ctggcaccgc ccagcggcgt gctgaaggag ctgtggcagc
     1921 gcgatgttgc gcgggatatg gattcggcag attatacaaa gctggcgact gaatttccgc
     1981 agtccttttg ctaaaggctt gtgtagtcgg gactttcggt gacgttctca ccgacacttg
     2041 agctcctgtc gcgacgaatc gccatgtaac aactagcaat ataacatcta tgccttatat
     2101 gcagtttcaa actcgacacg taagagttcg atcctctttc cccatcttgg cagtcaagaa
     2161 aatcttccta tactctccgc tttcgcagat cttcctgctt taacaatttg gaacgcctgg
     2221 gcaagggatt cgagctcggg cgaggattca ggagtcttcc atgtctgcat tttccgcagg
     2281 gatcgtcgta cacattgcgc ttctggccgg tggcgctcag ccttacgaac aggctcaaaa
     2341 acaagccgcc gccaacggcc agcctttgct cgttctggtg gggacggact ggtgccccgg
     2401 ctgccgcacg atgaaagacg gtgttttggc ccggatgtcg gccggtgggc ggctggcaaa
     2461 ggtaaactac acgatggtta atgcagacag ggaatcggac ctcgcggccc acttgatccg
     2521 cggcaacgcc attccgcagc ttatcgtgtt cagcaagcgc cccgatggcg aatggcaccg
     2581 cgagcaaatc accggcacgg cgtccgaaac ccaggtggca gctttgatcg agcgggcccg
     2641 cgctgcccag gcggcgaaga cggccaggcc aacgtcggct gcggtgggcg gcaactgatc
     2701 cgtcaatggc agcgtgcgac tgagcg
//
LOCUS       DASUGB010000547         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_339999, whole genome shotgun sequence.
ACCESSION   DASUGB010000547 DASUGB010000000
VERSION     DASUGB010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339999"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VFV87_10500"
     CDS             <1..1119
                     /locus_tag="VFV87_10500"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5104232.1"
                     /translation="PELAATYRAIADQGPDWFYRGPFAAAAEKWMVEHGGILSADDFA
                     AYKPIVRQPLVTKYRGRTVVGFPPPSSGGVHVAQILNILEQFDLAAIHARDPAEYQHV
                     LAEAMKLAFADRAHWLGDPAFARVPRGLVEKEYAQGLAAKINLAKATPVAGHGAPGDA
                     DERVFEKHTTHIAAADAEGNWVAITQTINTSYGSKVIVPGTGVVLNNEMDDFAIAPGT
                     PNAFGLVGAEANAVAPGKRPLSSMSPTIILEDGQPVMTLGAAGGPTIINQVVQAIVRR
                     FDLQLPLEECLAQPRIHHQWSPDLLRVESKLTEAEQKGLADRGHELTVTTAKIGVTQA
                     IEFDTTSDMFRGLADPRVPGKAAGIAEGERGRGGEGEK"
     gene            1116..2555
                     /locus_tag="VFV87_10505"
     CDS             1116..2555
                     /locus_tag="VFV87_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX5104233.1"
                     /translation="MTCATVTRCRNISLSPPLPLAPSTALALWLAHAAITFAQQPPAD
                     PPGRWQLELVTLKSGAEHQGLVQSQSEAEIDFAEIIQPPGKPMYAVIRGIPREAVAKL
                     DRLDDAAHEQLAARFRRFRHKAVIELGRMEQVELREETRDGSPHQVYDGSWFMLSSTA
                     DSERTRQCVVRIEQIFRAYRTLLPPRIDQPQPLFVTLYGSLDDYRSRLRRLDLSLDNA
                     AFYSPRERTILAGSDLNLFAERLDQVQGEHEEVKRNYARLDAEFAKTLATLSADLKAG
                     GFSDDEATAEIRQRKATWKAEMEKVLAANALQQRTNEQKFAEVTRQMFSRLYHEAFHA
                     YLDTFVYPHDSHHVPRWLNEGLAQVFESGQLDGDSLRIDAPDRDRLRRLREDLAVQPL
                     PLAQLLTAQEREFLGPHGDTSSQRHYLYAWGLAYYLAFDENLLGTPRFDAYVAGEAKG
                     LDPIVRFERLVNAPLPQFERRWREAMAVQ"
     gene            2714..>3204
                     /locus_tag="VFV87_10510"
     CDS             2714..>3204
                     /locus_tag="VFV87_10510"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104234.1"
                     /translation="MIRSLALSYAITLTATALSAVAFAADATGVRSRVFNGESLDGWH
                     VTGCEVGIENGLLVIKDGNGFVRTDGRYRDFILELDYKPRKAEMYDAGIYIRCELPPE
                     GKPWPSQYQVNLKQGDELNLIKFPKGRSTGLIKAGDWNHVKLTVTADKAAMEINGKPA
                     WET"
BASE COUNT          594 a         1066 c          993 g          551 t
ORIGIN      
        1 ccggaactgg cagctactta tcgagccatt gccgaccaag ggcccgactg gttctatcgc
       61 ggtcccttcg ccgctgccgc cgagaagtgg atggtcgagc acgggggcat cctctccgcc
      121 gacgacttcg ccgcctacaa accgatcgtc cgccagccgc tggtgactaa gtaccgcggc
      181 cggacggtcg tcgggttccc accgcccagc tccggcggcg tgcatgtggc gcagattctg
      241 aacatcctgg agcagttcga cctggcggcg atccacgccc gcgatccggc cgaataccag
      301 cacgtcctgg cggaggccat gaagctcgcc tttgccgacc gggcccattg gctgggcgat
      361 ccagctttcg cccgcgttcc gcgggggttg gtcgagaagg aatatgccca gggactggcg
      421 gcgaagatca atctggcgaa ggccacaccc gtcgccggcc acggtgctcc tggcgacgcc
      481 gacgagcggg tctttgagaa acacacgacc cacatcgctg ccgccgacgc ggaagggaac
      541 tgggtcgcaa tcacgcagac gatcaatacg tcgtatggct cgaaagtgat cgtccccggg
      601 actggtgtcg tgcttaacaa cgagatggac gacttcgcaa ttgcgcccgg tacgcccaac
      661 gccttcggtc ttgtcggcgc agaagccaat gccgtcgctc ccggcaagcg gccgctctcc
      721 agcatgagcc cgacaatcat cctggaagac ggccagccgg tgatgacgct cggagcggcc
      781 ggcggcccga cgatcatcaa tcaggtcgta caagccatcg tccgccgctt cgacctgcaa
      841 ttgccgctcg aagaatgcct cgcccagcca cgaatccacc accagtggtc gcccgacctg
      901 ttgcgggtcg aatcgaagct caccgaggcg gagcaaaaag ggcttgccga tcgcggtcac
      961 gagctgaccg tcaccacggc gaagattggc gtgacccagg cgattgagtt cgacacgacc
     1021 agcgacatgt tccgcggcct ggccgatccg cgtgtgccgg ggaaggcggc gggcattgca
     1081 gagggggaga gagggagagg gggagagggg gagaaatgac ttgtgccacc gttacacgtt
     1141 gccgaaacat ctccctctcg ccccctctcc ccctcgcccc ctcgaccgct ctcgccttgt
     1201 ggttggcgca cgccgcgatc acattcgcgc agcagccccc cgccgatccg cccggccgct
     1261 ggcagcttga attggtcacg ctcaaatccg gggcggagca tcagggtctt gtgcagtcgc
     1321 agtcggaagc cgaaatcgac tttgccgaga tcatccaacc tcccggcaag ccgatgtatg
     1381 cggtcattcg cggcattccg cgcgaggctg tggcgaagct ggaccggctg gatgacgccg
     1441 ctcacgagca gcttgccgcc cgctttcgcc gcttccgcca caaggcggtg atcgagctgg
     1501 ggcggatgga gcaagtcgag ctgcgggagg agactcgcga cggctcgccg caccaggtct
     1561 atgacggctc gtggttcatg ctgtccagca cggccgacag cgagcgaacc cggcagtgcg
     1621 tcgtccgcat cgagcagatt ttccgggcct atcgcacgct cctcccgccg cggatcgacc
     1681 agccgcagcc gctgttcgtc acgctctacg gctcactgga cgactatcgc agccgcctgc
     1741 ggcgcttgga tttgtcgctg gacaacgccg ccttctattc gccccgcgag cggacgattc
     1801 tggccggcag cgatctgaat ctgtttgccg aacgtctgga tcaagtccag ggcgagcatg
     1861 aggaagtcaa gcggaactac gcccggctcg atgcggagtt cgccaagacg ctcgccacgc
     1921 taagtgccga cctcaaagcc ggcggcttct ccgatgacga ggcgactgcg gagattcgcc
     1981 agcgaaaggc cacctggaag gctgaaatgg agaaagtcct ggccgctaat gccctgcagc
     2041 agcggactaa cgagcagaag ttcgccgagg tcacgcggca gatgttcagc cgtctctatc
     2101 atgaagcctt tcacgcctat ctcgacacat tcgtctatcc gcacgattcg caccacgtgc
     2161 cgcgctggct gaacgaaggc ctggcgcaag tcttcgagtc cggccagctc gacggcgatt
     2221 cgctgcggat cgatgcgccg gaccgcgacc ggctacggcg tctgcgggag gatctcgccg
     2281 ttcagccgct gccgcttgcg cagctcttga cggcccagga gcgcgagttt ctcggcccac
     2341 acggcgatac cagctcgcag cggcactatc tctacgcctg gggcctggcc tattatctcg
     2401 cgttcgacga aaacctcctc ggcactcccc gattcgatgc gtacgttgcc ggagaagcga
     2461 agggattgga tcccatcgtc cgcttcgagc gcctcgtcaa cgcgccgctc ccgcagttcg
     2521 agcgccgctg gcgcgaagca atggcagtac aataacccga agcgtcagcg agggagtgct
     2581 cacatcccca tggctccctc gctgacgctt cgggttggtg cgacttgcat cccccgtcgt
     2641 ggcgggccat gatgctgcat gtcggttttg cgccggcacc cgcccattcc ttcccgcctt
     2701 cggcgagccc tccatgattc gctctcttgc tttgtcgtat gcgattacct tgaccgccac
     2761 tgccttgtct gctgtggcct tcgccgccga tgcgaccggc gttcgctcgc gagtcttcaa
     2821 cggcgaaagc ctcgacggct ggcacgtgac gggctgcgag gtgggcatcg agaacggcct
     2881 cttggtcatc aaggacggca acggctttgt ccggaccgac gggcgctatc gcgacttcat
     2941 cctggaactg gactacaagc cgcgcaaggc ggaaatgtac gacgcgggaa tctacatccg
     3001 ctgcgaactg ccgccggaag gcaagccgtg gccgagccag tatcaggtca atctgaagca
     3061 gggggacgag ctgaacctga tcaagttccc gaaaggacgc agcaccggcc tgatcaaagc
     3121 gggcgactgg aaccatgtca aactgaccgt caccgccgac aaggccgcga tggagatcaa
     3181 cggcaaaccc gcctgggaaa ccga
//
LOCUS       DASUGB010000548         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1867695, whole genome shotgun sequence.
ACCESSION   DASUGB010000548 DASUGB010000000
VERSION     DASUGB010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867695"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1717
                     /locus_tag="VFV87_10515"
     CDS             287..1717
                     /locus_tag="VFV87_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104235.1"
                     /translation="MPLPADATKLAAATPGERQLEGPQQPAIALQKFSPAEIQVGKPA
                     LFEMLVRNTGAVAAQNVVVADHVPAGTQLLDAKPQPQQAGDGSLVWSLGTMQPGDEVA
                     ITLQVMPQQEGEIGSTAHVTFAAAATSRSICTRPQLAIEHTAPPKVLIGESLTVGITV
                     SNPGTGPATGIIIEEDVPDGLSHVAGAQLEYEVGTLRPGESKRLELSLKAEKAGLIQN
                     TIRVHGEGSLANQHAVQIEVVAPQLAVEVDGPKRRFLERQATYTLSIANPGTAAAREV
                     ELAAFLPRGMKFVDTDSQGQYDPAQHAVFWSLEELPASKAGAVKLTTLPIEPGEQRIR
                     IEGHANLGLAAEGEQIVQVEQSAELVHSVNDVEEVIEVGSETTYEIRVTNTGTKAATN
                     VRIAAQLPAEMQALGGEGPTRATGDAQKVIFEPLARLNPQEEVIFKIQVQGLKPGDHI
                     VRVQLASDEWPNPVTREESTRVYEDR"
     gene            1808..>3058
                     /locus_tag="VFV87_10520"
     CDS             1808..>3058
                     /locus_tag="VFV87_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104236.1"
                     /translation="MFGTLILVTGLVLGQNTEKAQAEKPAVEQQAAAANADLAKKVAA
                     LVRQLDSNQQAQREAAEKDLVELGPDVLPHLPALTPRTPAEVRNRLSRVRTALMKAEI
                     EAATKPAMVTLSGEMLFSEAAAQIAEQTGNKLIDYRERFNQEGSDPKIKVDVQGVPFW
                     EALDTVLDAANLTIYNYDEEQGALAYTSRGDNAVPRVGKASYSGLFRLEPTRVEAVRD
                     LRNSNTHSLKLVTDAIWEPRIRPIVLEFPLADVSATDESGSPIEIDRATGTLEVPADG
                     TNSAVEIEFPLVAPSRNVKQLASIKAKLTAVVLGKIESFEFADLDKLKSAELERGGVT
                     VIVESCRKNGDIYDVSMRVRFDKAANALESHRGWIYNNECYLLDPKGQRNENAGLEAT
                     LLDVNEVGLSYKFDLGEAAPAGHTF"
BASE COUNT          625 a          970 c          981 g          482 t
ORIGIN      
        1 cgagtcggcc ccgctctcat cggccgagac aactgcagta cctgctgatc ccgccgcggc
       61 ggtcgtgaat ccatattccg ccgattcgac gtacaatgca cctggcagcg acggaacggc
      121 atccggagac ggtatcgctg aatccactcc cgccgcggac gttcttgccg aacccactcc
      181 gctcggtggc gcaaagcctg cctacgattc ctcgtctgct ccaggcgcca aaccttacgg
      241 catgaggcgc agcgcgagtg aattggcctc ctcggctgac cgcgtcatgc cgctgccggc
      301 tgacgccacg aagctcgcgg ccgcgactcc tggcgagcga cagcttgaag gcccgcagca
      361 accggcgatc gccctccaaa aattctcacc ggccgaaatt caagtcggca agcccgccct
      421 gtttgagatg ctcgtccgca acacgggcgc cgtcgcggcc cagaacgtcg tcgtcgccga
      481 ccatgtcccc gccggcacgc agcttctgga tgccaaaccg cagccgcagc aagcgggtga
      541 tggctcgctc gtatggagcc tgggaacgat gcagcccggt gacgaggtcg ccatcacgct
      601 gcaagtcatg ccccagcaag agggcgagat cggcagcacg gcccacgtca ctttcgccgc
      661 tgccgccacc agccgcagca tctgcacgcg tccgcagctc gcgatcgagc ataccgctcc
      721 tcccaaggtg ctgatcggcg agtcgctcac cgtcggcatc acggtctcga acccgggcac
      781 cggcccggcg accggcatca tcatcgaaga ggacgtgccc gacgggctct cgcacgtcgc
      841 cggcgctcag cttgaatatg aagtcggcac gctccgcccc ggcgaatcga agcggctgga
      901 gctgtcgctc aaagccgaga aggccggcct gattcagaac acgattcgcg tccacggcga
      961 agggagcctg gccaatcagc acgcggtgca gatcgaggtc gtcgctccgc aacttgccgt
     1021 cgaggtggat ggtcccaagc gccgcttcct ggagcgtcag gcgacctaca cactgtcgat
     1081 cgccaatccc ggcacggctg cggcccgcga agtcgagctg gctgcctttc tgccgcgggg
     1141 gatgaagttc gtcgataccg actcgcaggg gcaatacgac cccgcgcagc acgccgtctt
     1201 ctggagcctg gaagagctac ccgcctcgaa agccggcgcg gtcaagctga ccacgctgcc
     1261 gatcgagccc ggcgagcaac ggatccggat tgagggtcac gccaacctgg gcctggccgc
     1321 cgaaggcgaa caaattgtgc aggtcgagca gtcagcggaa cttgtgcata gtgtcaacga
     1381 cgtggaagaa gtgattgaag tcggcagtga gacaacctac gaaatccgcg tgacgaacac
     1441 cggcaccaag gccgcgacca acgtccggat tgccgcccag ttgccggccg agatgcaggc
     1501 cctgggcggc gaaggcccca cccgagccac tggcgacgcc cagaaggtga tttttgaacc
     1561 gctcgcccgt ctcaatccgc aggaagaagt gatcttcaaa atccaagtcc agggcttgaa
     1621 gcccggcgac cacatcgtcc gcgtccaact cgccagcgac gaatggccga atcccgtgac
     1681 ccgcgaagag agcacgcggg tttatgaaga ccggtagacc aaagcggtag cgtctggagt
     1741 cgctgcgcgc ggggccgtag aataaggagt cggctgcaga aactgtttct tcccagggag
     1801 ccccagcatg ttcggaacat tgatcctcgt taccgggctc gtgctagggc aaaatactga
     1861 aaaggcccag gcggaaaagc cggctgtgga gcagcaagcc gctgccgcaa atgcggacct
     1921 tgccaaaaag gtggcggcgc tggtgcggca gctcgatagc aaccagcagg cgcagcgcga
     1981 agcggcggaa aaggatctgg tcgaactggg cccggacgtc ctgccgcact tacccgcgct
     2041 gacgccgcgc acgccggcgg aggtccgcaa ccggctgtcg cgcgtccgca cggccctgat
     2101 gaaggccgag atcgaagcgg cgacgaagcc cgccatggtg accctctccg gcgagatgct
     2161 gttttccgaa gccgcggcgc agattgcgga gcaaaccggc aacaagctga tcgactatcg
     2221 cgagcggttc aaccaggaag ggagcgatcc gaaaatcaag gttgatgtac agggcgtccc
     2281 gttttgggag gcgctcgata cggtgctcga tgcggccaat ctgacgattt acaactacga
     2341 cgaggagcaa ggagcgctgg cctatacttc gcggggcgat aatgccgtgc cgcgtgtggg
     2401 gaaggcgagc tacagcggcc tgtttcgtct ggagccgacg cgggtcgagg cggtccgcga
     2461 tctgcgcaat tccaatacgc actcgctgaa gctggtgacc gacgcgatct gggaaccgcg
     2521 aatacggccc atcgtgctgg aattcccgct ggccgacgtc tcggcgaccg acgaaagcgg
     2581 gagcccgatt gagatcgacc gggccaccgg caccttggaa gtgccggcgg acggaacgaa
     2641 ctcggccgtc gagattgagt ttcccttggt cgcgccgtcg cggaatgtga agcagcttgc
     2701 gtcgatcaag gcgaagttaa cggcggtcgt gctcggcaaa attgaatcgt ttgagttcgc
     2761 cgacctcgat aaactgaaat cggccgaatt ggagcgcggc ggtgttacgg tgatcgtcga
     2821 aagctgccgc aagaacggtg atatctacga cgtcagcatg cgggttcgct tcgataaggc
     2881 ggccaacgcg ctcgagtcgc atcgcggctg gatttacaac aacgagtgct acctgctgga
     2941 tccgaaggga cagcggaacg agaacgccgg gctggaggcg acgctgctgg acgtgaacga
     3001 ggttgggctg tcgtataagt tcgacttggg tgaggcggcg cccgccggcc acaccttc
//
LOCUS       DASUGB010000549         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1173681, whole genome shotgun sequence.
ACCESSION   DASUGB010000549 DASUGB010000000
VERSION     DASUGB010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173681"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(537..1277)
                     /locus_tag="VFV87_10525"
     CDS             complement(537..1277)
                     /locus_tag="VFV87_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104237.1"
                     /translation="MIQPSRILASLVVTLLLSTAGGAETDNAPFDGEWSLEFRVNTRP
                     GGASFTTTQGLAVSPYHFNFRAPIESFRFQDHRRVRLEQREDSTLSIRSTTRISTVTL
                     WDRQNHQGRIHVDGAGSGEAADRRLKLTIHWGLSSGTGFSSDNFGTSQTTRALMSEDG
                     TQITLSNEAGTRTFGFDLWKSTWELKPAAIEKRTVGEDVIEETTTFRGQRNVSITPLD
                     PGGFTPALPVIEAIELKQFRQLKLVPRG"
     gene            complement(1280..2701)
                     /locus_tag="VFV87_10530"
     CDS             complement(1280..2701)
                     /locus_tag="VFV87_10530"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5104238.1"
                     /translation="MAVGLPFVDGGLLLAQQAAMPASTLKELEKQAAAGEAAAMFALG
                     ERFRLGLDTEIDLRKAAEWYGKAAQNDHAAAMTQLGRMYRNGQGVGKNDLEAINCFRR
                     AVELEHPPAMNYLGMMYVSGRGALPDAARAAKLFRQAADKGDALAMINLADALAAGRG
                     IQADAAQAKTWYAKPRETFEKAANTGDGDAMLGLVTILRYGKGTEAHPDQAAAWCQKA
                     ADQGRADAMFLLGRMYLDGEGVAQDADQARSWWLKAAERGDPECMYLLAVGHRTGKPF
                     AKDAKQSLVWCQKAAAAGHAEALNMLGELHAAGDGVPKDDKKAHEWFLQAAQLGDGPA
                     LRHIGEFYAAGRAVTKDNQAAVSFYRSAADAGDSSGAYELARCYADGRGVTKDEAQSA
                     HWFSQAAFGGHAAAGYELGRLYADGRGVSQKAEMAAFWYRAAANQGNVPAMKALSEAL
                     RTGMGVAKDEKQAAEWRAKAEQE"
     gene            complement(2781..3407)
                     /locus_tag="VFV87_10535"
     CDS             complement(2781..3407)
                     /locus_tag="VFV87_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104239.1"
                     /translation="MRLISTILALLLCSAWHCPSARGQGNVSRTYTGTNGPEGRAPFR
                     PTGPGSTGDHFIANIYGDAIEVLSQGGDQCGRPFYFTGTIAIQPNQTKGTIGGPMLRC
                     TNEELKAACAKVGIRLTDYYEVGYTGTVERNPDQGAYLIKITYPYTIWVKDDCKEKRD
                     TQGTDTILLVYQPPREDPSWGEALHRGERRMVDGIVDQLEGGKLFQHY"
BASE COUNT          626 a         1093 c         1007 g          708 t
ORIGIN      
        1 ttgaacaccg tccccgtgtc gacgccgtag ttgaaaaaga ccaccagggc cgctcgccag
       61 tgctgatgga catgacgacg actccccggc cgtcggcatc cgcatcccgt acccggctcc
      121 gtcagccgac agcccttgga acggcctcct gcatggcctc ccgccgcttg gctgatgaag
      181 gcgcaaccca cgcaggttgc acagatgcgc ccaggttagt cagacttagg gcgaaaagcg
      241 ctgctctgca actgcatgtt cccggcgggc gatggaaccg cctgcagttc tggctggggc
      301 acactactgg cggtcttctt caccgccgca gttacacttg cggctgggcc ttccgagggc
      361 gaccgctgta tctcggcgga atcagggctg aaatgaccga ctctactctc gcagattgag
      421 cgacccgggc gcggatgcaa ggcgaatagt cggagtctgt tcgtaacaat caagacctgt
      481 tgctttccaa gtctgcgaca ataattgcga tggcgcgtcc tgcaggacga gatagtctac
      541 cctcgaggaa ccaacttgag ctgccggaat tgcttcagct cgatagcttc gatcacgggt
      601 aatgcgggcg tgaagccgcc aggatcgagg ggagtaatgc tgacattccg ctgaccgcga
      661 aaggtggtcg tctcttctat cacgtcttcg cccacagttc gcttctcaat ggcggcgggt
      721 ttaagttccc atgtcgactt ccacaaatca aatccaaatg tgcgtgtccc cgcttcattg
      781 ctgagcgtaa tctgggtgcc gtcttcggac attagcgctc gagtggtttg cgaggtgcca
      841 aagttgtcgg acgaaaaccc cgtaccgctg gacaatcccc agtgaatcgt cagcttcagc
      901 ctgcggtcgg cggcttcacc ggatcccgca ccatcgacgt gaattcggcc ctggtgattc
      961 tgacggtccc acaacgtgac cgtgctgatg cgcgtagtgg agcggatact cagggtagaa
     1021 tcttcgcgct gctcaagtct gacgcgccga tggtcctgaa accggaacga ttcaattggc
     1081 gcccggaagt tgaagtggta aggcgaaact gccaaccctt gcgtggttgt aaacgaggca
     1141 cctccgggcc tggtgttgac tcgaaattct aacgaccact ctccatcgaa aggggcattg
     1201 tccgtttcag cacctccggc ggtcgagagc aatagtgtga ccacaagcga ggcgagaatc
     1261 ctgcttggtt gtatcatgac tactcctgct ctgccttggc acgccactcg gcggcttgtt
     1321 tttcatcttt cgcgacaccc attccggtgc gaagggcctc gctgagagcc ttcatcgcgg
     1381 ggacattccc ctggttcgcc gcggcgcgat accagaacgc cgccatttcg gccttttggc
     1441 tcacgccgcg accgtcggca tagagccgac ccagttcata gcccgcggcg gcatgtccgc
     1501 cgaaggcggc ttggctgaac cagtgcgccg actgcgcctc gtccttcgtc accccgcgcc
     1561 catcggcgta gcagcgggcc agctcgtagg ctccagacga gtcgcctgcg tccgcggcgc
     1621 tgcgataaaa gctgacggcg gcctggttgt ccttcgtaac ggcgcggcca gcggcgtaga
     1681 actcgccaat gtgccgcaga gccggaccat cgccaagctg tgcggcttgc agaaaccact
     1741 cgtgtgcttt cttgtcgtcc ttcggcacgc catcacccgc ggcgtgcaat tcgcccagca
     1801 tgttcaatgc ctccgcatgt cccgccgctg ccgccttttg gcaccaaact agcgattgct
     1861 tagcatcttt ggcgaacggc ttaccggtcc gatggcccac cgccaagaga tacatgcact
     1921 cggggtcgcc gcgctcggca gccttgagcc accaggaccg agcctggtcc gcgtcttgag
     1981 caacgccttc accatcgaga tacatccgcc cgagcaggaa catggcatcc gcgcggccct
     2041 ggtccgcggc cttctgacac caggcggctg cctggtccgg atgtgcctcg gttcccttgc
     2101 catagcgaag gatcgtgacc aagccgagca tcgcgtcgcc gtcgcccgtg ttggcagctt
     2161 tctcgaaggt ttcgcggggc ttggcgtacc aggttttcgc ctgcgccgcg tcggcctgga
     2221 tgccgcggcc cgcggcgagc gcgtcggcca ggttgatcat cgccagggca tcacccttgt
     2281 cggcagcctg gcgaaacaat ttcgcggcgc gggcggcgtc gggcaaagca ccccgaccgc
     2341 tgacatacat cattcccaga tagttcatcg ccggcggatg ttccagctcc acggcccgcc
     2401 gaaagcaatt gatcgcttcc aggtcattct tgccgacgcc ttggccgttg cgatacatcc
     2461 ggccgagttg cgtcatcgcc gcggcgtgat cattctgcgc ggctttgccg taccactccg
     2521 ccgccttacg gaggtcgatc tcggtatcca atccgagccg gaatcgctcg cccagagcga
     2581 acatcgctgc cgcctcaccc gccgcggcct gtttttccag ttctttgagt gtggacgccg
     2641 gcatcgcagc ttgctgtgct aacaagagac cgccatcgac gaagggcagg ccaactgcca
     2701 taagtgctgc cgttgccaac ggaattgacc attgatgaaa catcgcgaga ttcataggtg
     2761 cgtcctccac ggtggcgctg tcaatagtgc tgaaacagct tgcccccctc gagttgatcg
     2821 acaattccat ccaccatgcg gcgctcaccg cgatgtagcg cctcgcccca ggaaggatcc
     2881 tctcgtggcg gctgatagac aagcaggatc gtgtcggtgc cctgcgtgtc gcgtttctcc
     2941 ttgcagtcgt ccttgaccca aatggtgtag ggataggtga tcttgatgag gtaggcgccc
     3001 tggtccgggt tgcgctcgac cgtgccggtg taaccgacct cgtaataatc ggtcaggcga
     3061 ataccaactt tggcgcaggc cgctttcagc tcttcattgg tacagcggag catcgggcca
     3121 ccgatcgttc ccttggtctg gttcggctgg atggcgatcg tgccggtaaa atagaacggc
     3181 cgcccgcact ggtccccgcc ctgcgagagc acttcgatcg catcgccata gatgttggcg
     3241 ataaagtgat ccccggttga gcccgggccc gttggtcgaa acggcgcccg gccctccggg
     3301 ccgttggtac ccgtataggt ccgcgagaca ttgccttggc ctcgcgccga gggacaatgc
     3361 caggcactgc acaacaacag ggccaagatc gtggatatga gtctcataaa gagttctccg
     3421 aagtcttggt agcg
//
LOCUS       DASUGB010000550         5138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_617269, whole genome shotgun sequence.
ACCESSION   DASUGB010000550 DASUGB010000000
VERSION     DASUGB010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617269"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFV87_10540"
     CDS             complement(<1..897)
                     /locus_tag="VFV87_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5104240.1"
                     /translation="MSCRPSRSARRLMLEPLEPRLALTTFYVSNLGADTGSGSQAAPW
                     ATLQKAANTVQAGDTVIVRSGNYVGFHMTRDGTATARITFQAEPGAAVTQRNATTPDG
                     INLEGADYVTIDGFAVNGMPRAGIRSVTNHDVIIRNNHLDQNYKWGILTGFSDDLLIE
                     NNVASRSQVEHGIYVSNSGDRPVIRGNTIWGNNANGIHMNGDASLGGDGIITGALVEN
                     NVIYDNGLGGGSGINGDGVQDSVFRNNLLYDNHASGISLYQIDGGGGSTGNVIVNNTV
                     IQASNGRWALNIQSGSTGNTVRN"
     gene            complement(1118..2356)
                     /locus_tag="VFV87_10545"
     CDS             complement(1118..2356)
                     /locus_tag="VFV87_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104241.1"
                     /translation="MLCLTTLLPPQWSCADDSSSADLSALAGLLELVIEADADSAKQC
                     LQTLGAKIRSGEADAATLAELKKRLAPSLEKILAGKADDPLAADAAFVAALWKDRAAL
                     ARVESIATSGDPDLTQLRIALETLVFAKDEAGMVIASFMAQNSPPEYQRIALGALSRA
                     DGPRMAQAVVAAYPTLSAENQPLAIEVLTQRASWSKLLVAEVAAGRLQPGVLNVNQVR
                     KLLSLEDQELSAAVAKHWGTVREARDPAREQLVAEMKKLIRATPGDPHRGIAVFTKLC
                     GQCHKIHGQGQEVGPDITANGRASFEQLLSNVFDPSLVIGASYQARTVRTIDGRVITG
                     LLAEDNDQRVVLKVQGGKLESIARENVEAMKVSELSLMPEGIEKQLQPQEIADLFAFI
                     TLDKHPDDKTSRLIPGTRAD"
     gene            complement(2476..4338)
                     /locus_tag="VFV87_10550"
     CDS             complement(2476..4338)
                     /locus_tag="VFV87_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5104242.1"
                     /translation="MRLLLAFLLATFIPLMAAAADSVIPHAQDKPPGPPLSPAEAMAK
                     MKVPEGFTVELVASEPNLVNPVAFTFDEKGRIWIAESLEYPRKQAGKGQDRIKILEDT
                     DADGKADKFTIFAEGLNIPSGIAVGHGGVWIANAPDILFYPDADRDGVADSEPQAIVT
                     GFGRLDTHELPNSFTWGPDGWLYGLNGVFNNSHVKYAAENPNFKTDHPGWQITCALWR
                     IHPQTREFQVFCEGTSNPWGVAIDGEGSFFVSACVIDHLWHLVETGYYHRQGGPYPPF
                     TWKIDAMPQDKHQKAAYCGIHWFDSDAYPEPYRGKLFMGNIHGNCINVDSVKRDGSTF
                     ASKGEPDFLTADDAWFMPVGQETGPDGSLYILDWYDRYHCYQDANRDPNGIDRLKGRL
                     YRIRYKDTPRASGFDLSKESDDQLLERLGAGNDFIRSTAQRLLYERQLADINSMAGIP
                     KRIGTREKLRQLIEDKTMPQRQRAHALWVLVSTWEIDEGQTMYLLKNAEPWVQAWIVR
                     LLGNVGGASPAQIETIRKLGREGSPEVKLQVAIAARKIDGVDALPVLVEVLAHAGDDK
                     MIPHIVWQNLHPLLETKSDQFLAEVKKYDLKETPSLAKIMPRVTERILAAKKSK"
     gene            4452..4940
                     /locus_tag="VFV87_10555"
     CDS             4452..4940
                     /locus_tag="VFV87_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104243.1"
                     /translation="MTDDLLPSTANDPHWTTSWEQLGQGVPTLAGLAQVASRAVASGG
                     DKTIRLSMEARCLLFAAKDRGILEIKGSNRAFESPARMLAVHVETGPDRTLIFRSRDK
                     PMMTIRFLAGFRELCASGLVMHHIFTEFSLSREGLELANSIPEAEIGSLLATAREICG
                     ME"
BASE COUNT          913 a         1553 c         1608 g         1064 t
ORIGIN      
        1 gttgcggacc gtgttgcccg tgctgccgct ctggatgttg agagcccacc gcccgttcga
       61 tgcctggatg accgtgttgt tcacgatcac gttgccggtc gaaccaccac cgccgtcgat
      121 ttggtagagc gagatgccac tggcgtgatt gtcgtaaagc agattgttgc ggaagaccga
      181 gtcctgcacg ccgtcgccgt tgattcctga cccgcctccc aagccgttgt cgtagatcac
      241 gttgttctcg accaaggctc ccgtgatgat gccgtcgccg cccagggagg cgtcgccgtt
      301 catgtgaatg ccgttcgcgt tgttgcccca aatcgtattg ccgcggatca cgggccggtc
      361 gccactgttg gagacgtaga tcccatgctc gacctgcgag cgtgacgcca cgttgttctc
      421 aatgagcaga tcatcggaga agccggtcag aatcccccac ttgtagttct ggtccagatg
      481 attgttacga atgatcacgt cgtgattcgt caccgaacgg atgccggccc gcggcatgcc
      541 attgaccgca aaaccgtcaa tggtgacgta atcggcccct tccagattga ttccgtcggg
      601 cgtggtcgca ttccgctgcg tgacggccgc gcctggctcc gcctggaagg taattcgtgc
      661 ggttgccgtg ccgtctcgcg tcatatgaaa gccgacgtag ttgccggatc ggacgatcac
      721 ggtgtcgccg gcctggacgg tattggcggc cttttgcaat gtcgcccagg gggcagcctg
      781 cgagccgctg ccggtatcag ctcctaaatt tgagacatag aacgtcgtca gcgccagacg
      841 cggctccagc ggttccagca tcaaacggcg cgcagaacga gacgggcggc aggacatggg
      901 caggtctcct gtgcctgcga ggacgaggac tctgttccag acgaggactc tgttccattc
      961 gactcgcgct accgggaatg cggcagcgag cgcgcaagtc gcaaggtcta tgccaaccct
     1021 agcggattct tgaaaacgag gcaaaccgac aagcagcttg acttcccaga ttccattcgt
     1081 tcgctagcaa gccagggatt cccagtcccc agcggcatca gtcggcccgc gtccccggaa
     1141 tcagacgcga cgttttgtcg tccgggtgct tgtcgagagt aatgaaggcg aacaagtcgg
     1201 cgatttcctg cggctgaagc tgcttctcga ttccctcggg cattagcgac agctcgctga
     1261 ctttcatcgc ctcaacgttt tcgcgggcaa ttgactccag tttgccgcct tggaccttga
     1321 ggacgacgcg ctggtcgtta tcttcggcta agaggcccgt gatgacgcgg ccatcgatcg
     1381 tgcgtacggt gcgggcctgg tacgacgcgc cgatcaccag gcttgggtcg aacacgttgg
     1441 agagaagttg ctcgaacgaa gcccggccgt tggcggtgat gtccgggccg acttcctgcc
     1501 cctggccgtg aatcttgtgg cactggccgc agagtttcgt aaagaccgcg atgccgcggt
     1561 gcggatcgcc gggcgtggcg cgaatcagct tcttcatctc ggccacaagc tgttcgcgcg
     1621 ccggatcgcg agcttcgcgg accgttcccc aatgcttcgc caccgcagcg gacaactcct
     1681 gatcttccag cgaaagcaac tttcggacct ggttcacgtt cagcaccccc ggctgcaagc
     1741 ggcctgcggc gacttccgcc accagcagct tgctccagga agcacgttgc gtgagcacct
     1801 cgatggcgag gggctgattt tccgcggata gcgtgggata ggccgcgaca acggcctggg
     1861 ccattcgcgg cccgtctgcc cgtgacaagg cgccgagggc aatccgttgg tactcaggcg
     1921 gggagttttg cgccataaag ctggcgatca ccattccggc ttcatccttc gcaaacacga
     1981 gtgtttccag cgcaattctc agttgcgtca ggtctgggtc gccactcgtc gcaatggatt
     2041 ctacgcgagc aagggcggcg cgatccttcc acagcgcggc gacaaatgct gcatcggcag
     2101 caagcggatc atctgccttg ccagcgagaa ttttctccag cgacggagcc aatcgcttct
     2161 tcaactcggc cagcgtcgcc gcatccgctt cgccgctgcg aatcttcgcg ccgagcgtct
     2221 gcaggcattg cttcgccgag tccgcgtccg cttcaatgac gagttccagc aggccggcga
     2281 gtgcggagag gtcggcgctc gacgagtcgt cggcgcaaga ccactgaggc ggcaggagtg
     2341 tggtgagaca gagcatcaca cagaggcgca gaggcacaga ggaataagtc ctccgagctt
     2401 ctgcctctct gtgcctcagt gcctctgtgt gagatttcat aaaagtgggc agcgacggca
     2461 cacggcgtgt gcctactact tggacttctt cgccgcgagg atgcgttcgg tgacgcgagg
     2521 catgatcttg gccaggctcg gcgtttcctt gaggtcgtac ttcttcactt cggccaagaa
     2581 ctggtccgac tttgtctcca agagggggtg caggttttgc cagacgatgt gtgggatcat
     2641 tttgtcatct ccggcatggg cgaggacttc gacgaggacc ggcagggcat cgacgccgtc
     2701 gatcttgcgg gcggcaatgg cgacttgcag cttcacctcg ggcgaacctt cgcggccgag
     2761 cttgcgaatc gtttcaatct gcgccgggct ggcgccgccg acattcccga gcaggcgcac
     2821 gatccaggct tggacccacg gctcggcgtt tttcagcagg tacatcgtct gcccttcgtc
     2881 aatttcccag gtgctgacga gcacccacag ggcgtgggct cgctggcgct gcggcatcgt
     2941 cttgtcttcg atcaattgcc gcagcttttc gcgcgtcccg atccgcttcg gaatgccggc
     3001 catcgagttg atgtcggcga gctgccgctc gtagagaagc cgctgagccg tgctgcggat
     3061 gaaatcattg cctgcgccga gccgctcgag gagctggtcg tcggactcct tcgagaggtc
     3121 gaagccggag gctcgcgggg tgtctttgta acggatgcga tacagccggc ccttcagtcg
     3181 gtcgatgccg ttgggatcgc ggttggcgtc ctggtagcag tgataccggt cgtaccaatc
     3241 gaggatgtag aggctgccgt cgggaccggt ttcctggccg acgggcatga accaggcgtc
     3301 atcggccgtg aggaaatcgg gctcgccctt gctggcgaac gtgctgccgt cgcgtttgac
     3361 cgaatcgacg ttgatgcaat tgccgtggat gttgcccatg aagagcttgc cgcgatacgg
     3421 ctcgggatac gcgtcgctgt cgaaccagtg aatgccgcag taggcggctt tctgatgctt
     3481 gtcctgcggc atcgcgtcga tcttccaggt gaacggcgga taggggccgc cctggcggtg
     3541 gtaatagccg gtttcgacca ggtgccagag gtggtcgatc acgcaggcgc tgacgaaaaa
     3601 cgagccttcg ccgtcgatcg ccacgcccca ggggttgcta gtaccctcgc agaagacctg
     3661 gaattctcgc gtctgcgggt gaatccgcca cagggcgcac gtgatctgcc agccggggtg
     3721 atcggtcttg aagttcggat tctccgcggc gtacttcacg tggctgttgt tgaacacgcc
     3781 attgaggcca tacagccagc catcgggccc ccaggtgaag ctgttgggca attcgtgcgt
     3841 atccagccgc ccgaaaccgg tgacgattgc ctgtggctcg ctgtcggcaa cgccgtcgcg
     3901 gtcggcgtcg ggatagaaaa ggatgtcggg ggcgttggcg atccacacgc ccccgtggcc
     3961 gaccgcgatg ccgctgggga tattcagccc ctcggcgaag atcgtgaact tgtccgcctt
     4021 accgtcggcg tcagtgtctt ccaggatttt gatccggtcc tgaccctttc ctgcctgctt
     4081 gcggggatat tccaggctct cggcgatcca aatgcggccc ttttcgtcaa atgtgaaggc
     4141 caccgggttc accaggttcg gctcgctggc gacaagctcg accgtaaatc cttccggcac
     4201 cttcattttc gccatcgcct ccgccggcga gagcggtggg ccaggcggct tgtcttgcgc
     4261 gtgggggata acactgtcgg cagccgcggc catgagcggt atgaatgtgg caagcaagaa
     4321 ggccaagagg agtcgcatgc ggattggttc ccggtgaagg cgtcgtttcg attatgcggt
     4381 ctgagcggca gagatgccag ccacacaccg caggtttgca tctcgcggag cgctgcgact
     4441 acgatagcac catgaccgac gatctgcttc cctcgaccgc gaacgatccg cattggacca
     4501 cctcctggga gcaactcggc cagggggtcc cgacgctggc tggcctcgcc caggtggcca
     4561 gccgggcggt ggcgagcggc ggcgacaaaa cgatccgcct atcaatggag gcgcgctgcc
     4621 tgctgtttgc tgccaaggac cgtgggattc tggagattaa aggctccaac cgggcctttg
     4681 aatcgccggc gcgaatgctt gccgtccacg tcgagaccgg acccgaccgc actctcatct
     4741 tccggagccg tgacaaaccg atgatgacga tccgcttcct cgccggcttt cgggaactgt
     4801 gcgcctccgg tctggtgatg caccacattt tcacggagtt ttcactgtcc cgcgagggtc
     4861 tggaactggc gaattcgatt cccgaggccg agattggcag cctgctggca acggcccgcg
     4921 aaatctgcgg aatggagtag aatgaaatcg cggatcgcat tcacttgtag ctagagcacc
     4981 caatggaagt cgcaagcacc acagcaccgt acgaactcat cgactttgcc gagcttcctg
     5041 gtaatccttg cccgtgcgga accgcgcggc gggcacttgc tgatgtcaaa gactttcctg
     5101 gtacgatcca tgtgacggaa attgccctcg acaatcag
//
LOCUS       DASUGB010000551         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_480062, whole genome shotgun sequence.
ACCESSION   DASUGB010000551 DASUGB010000000
VERSION     DASUGB010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480062"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFV87_10560"
     CDS             <1..483
                     /locus_tag="VFV87_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104244.1"
                     /translation="TLRFLRDERLARLKIVQGWDPHLARALLDGPEHDAQHRQRCGVT
                     SGPMSFAVYQYLEILAAAKSGNWDEVAKSQAAVTVLFQAMQDDPGKFADLQRAKYIMG
                     LGHPLTGQVTPQQVERVFAALASLPRLDDRSRLARSLDLMGDGPYHQRLKSMSGIPGL
                     "
     gene            541..918
                     /locus_tag="VFV87_10565"
     CDS             541..918
                     /locus_tag="VFV87_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX5104245.1"
                     /translation="MSDTTSTLAELRDLVRRFVDERDWRQFHSPKNLSMSLAIEAAEL
                     MEHFQWIDVAESRGLKDQPDNLLAVREELADVLCYALALANELEIDLSAAVRDKMVKN
                     AAKYPAELSRGRYSPPAGTHSDE"
     gene            930..2150
                     /locus_tag="VFV87_10570"
     CDS             930..2150
                     /locus_tag="VFV87_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104246.1"
                     /translation="MNLSRREMLESLGIAAAGLVTAGYTGTARGFAANEEITVGCIGT
                     GGRCRRLMQSLATIPGVKIAAVCDVWDYHLAEGKKLAQPNAFETKDYHALLARKDIDA
                     VLIGTPDHWHVPITVDACAAGKDVYVEKPLTHDLSEGQRAIDAQNKHSRIVQVGTQQR
                     SMPHLQKAYEIVKSGQLGEIHKVHLTWNRNAERSARPNLNIDPKTVDWKRFLGTAKDQ
                     PFDEYRFRQWRWFWDFGGGIFTDLMVHWIDVVHWYLDLDHPALATSIGDHYQAEGLWE
                     TPDTVQTLMKYPQAGVQAYFEGTFVNARNAAMCEFMGSEATLYIDRGRYEIHPERKSK
                     LKASELILGEGPRGADFYNQPDGELLHLSNWIECIRSRKKPACPAEAGVSSASAAHMA
                     NLALRSGNVAKWNG"
     gene            2310..3659
                     /locus_tag="VFV87_10575"
     CDS             2310..3659
                     /locus_tag="VFV87_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104247.1"
                     /translation="MLRSAWVAIGLVLAVAALAEGKERIWTNSAGQSMRAEFVREVDG
                     EVTFLKGGKIVSVPLDSLSERDQQIVRDLAAGKPVSDDAPAAPGASPFSGEGPISGPA
                     TPGAEANREPLIKKPIPIENRTWTDIHGNQITGKFVRIFGSNVVIHRASRSVTVKFFE
                     LGSEDQEYVRELLTSRGQEGLIPPKVDVEEGTGDTAGGESAPAEEPTATESLPAAAAP
                     ASPQVPRGPSFGPGFPGGAPPGPSFPGRGPGFPSPGADPPGVGAGGYTPPSYTPPTPA
                     APSSFPSSDPYAAPGSRIGSGYADAQLERMRQQNEENQRRQAESMQRMQDRMSAQHES
                     FMNRFKKQGECLNCKHSLTEAEFNRTSCPHCGVTWDYEVDEFGHKRELHNSSYSSPLS
                     RTNNGEPLIDARSARQVGMVLGIFLGLAVVVGMIIGVIYIAMTIASASSSSQQRHYR"
     gene            complement(3678..>4216)
                     /locus_tag="VFV87_10580"
     CDS             complement(3678..>4216)
                     /locus_tag="VFV87_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104248.1"
                     /translation="LKVKDELTLESTSVQPRYVAASQGGKQIGVLFQSGTLWLIDSET
                     GTARRAPISRQGDIAGFAFSGERLLIGDVSNRVVAYDARSFTRQQEYRPAMSTAELAY
                     YYGIKPLHLVSPKPRHLDNTVKYALTGKRTTDLGLFQGNLAQKRDDLHPWRPVASGLA
                     FVGLMLLVSCVYLEWHEF"
BASE COUNT          812 a         1338 c         1354 g          712 t
ORIGIN      
        1 acgctgcggt tcttgcgtga tgagcgcctg gcccggctga agatcgtgca aggctgggac
       61 ccgcacctgg cacgcgcgct gctggacggt ccggagcacg acgcgcagca ccggcagcgg
      121 tgcggcgtca ccagcgggcc aatgtcgttc gccgtctatc agtacctgga gatactcgcg
      181 gcggccaaga gcggcaactg ggacgaggtt gccaagtcgc aggccgcggt gacggtgctg
      241 ttccaggcca tgcaggacga ccccggcaag ttcgccgacc tccagcgggc caaatacatc
      301 atgggcctcg gccatccgct gacagggcag gtcacgccgc agcaagtgga gcgcgtcttc
      361 gccgccctcg cttcactgcc gcgactcgac gaccgcagcc gcctggcccg cagcctggac
      421 ctgatggggg acggccccta ccaccagcgg ctgaagtcga tgtccggcat cccggggctt
      481 tgattaggtt cgacaaaccc gcctttcgcg gaacgccaca gagggcgttc cctacagcgc
      541 atgtccgaca cgacgtccac gctcgccgaa ctccgcgacc tcgtccgccg cttcgtggac
      601 gagcgcgact ggcggcagtt ccactcgccc aagaacctga gcatgtcgct ggccatcgaa
      661 gccgccgagc tgatggagca ttttcagtgg atcgacgtgg cagagtcgcg cggcctgaag
      721 gatcagccag acaatcttct ggcggtgcgc gaagaattgg ccgacgtgct ctgctacgca
      781 ctggccctgg ccaacgaact ggagattgat ctttcggcgg cagtgcggga taagatggtc
      841 aagaacgcgg ccaagtatcc ggcagagttg tcgcgcgggc gctactctcc gccggccggc
      901 acccactcgg acgaatagga gcaaacccca tgaatctctc tcgccgtgaa atgctcgagt
      961 ccctgggaat cgccgctgcg gggctggtca ccgccggcta tacgggcacg gcccggggct
     1021 ttgccgccaa cgaggagatc actgtcggct gcatcggcac gggggggcgg tgccggcggc
     1081 tgatgcaatc gctggcgacg attccgggcg tcaagattgc ggcggtctgt gatgtgtggg
     1141 actatcacct cgcagaaggc aagaagctgg cccagccgaa tgccttcgag acgaaggact
     1201 atcacgcgct cttggcccgc aaggatatcg acgcggtgct gatcggcacg cccgaccatt
     1261 ggcacgtgcc gatcacggtc gatgcctgtg cggcgggcaa ggacgtctac gtcgagaagc
     1321 cgctgacgca cgacctgtcc gaagggcagc gggccatcga cgcccagaac aagcacagcc
     1381 gcatcgtgca ggtcggcacg cagcagcggt cgatgccgca cctgcagaag gcatatgaga
     1441 tcgtgaagtc cggccagctc ggcgagattc acaaggtcca tctgacgtgg aaccgcaacg
     1501 ccgagcgctc ggcgcggccc aacctcaaca tcgacccgaa aaccgtcgat tggaagcggt
     1561 tccttgggac cgccaaggac cagccgttcg atgaataccg cttccggcag tggcggtggt
     1621 tctgggattt cggcggcgga atcttcaccg acctgatggt gcactggatc gacgtggtcc
     1681 actggtattt ggacctcgac catcccgcgc tggcgacgag catcggtgac cactaccagg
     1741 ccgagggcct gtgggaaacg cccgatacgg tccagacgct gatgaagtat ccgcaggcgg
     1801 gcgtgcaggc gtatttcgaa ggcacgttcg tcaacgcccg caacgccgcg atgtgcgagt
     1861 tcatggggag tgaggcgacg ctctacatcg accgcggccg ctatgaaatc catcccgagc
     1921 gcaagagcaa gcttaaggcg agcgagttaa tactgggcga aggcccgcgc ggcgccgact
     1981 tttataacca gcccgacggg gagctgctgc acctgtcgaa ctggattgag tgcatccgca
     2041 gccgcaagaa acctgcctgc ccggctgaag cgggagtgag ctccgcctcg gctgcccaca
     2101 tggccaattt ggcattgcgg agcggcaacg tggcaaaatg gaacggctag ctgtggacta
     2161 gagattgcgg ctgagaatct agtcaagccg atctcaaaac gcaggctgca ctttgacaac
     2221 gcctggtagg cttgtcacac tatcagggga ttgctcgtcc cttatcggct ttcgtcatta
     2281 cattctcgtc attttcaagg acctctccta tgctgcgcag cgcttgggtt gcgattggat
     2341 tggtgctggc cgttgcggcc ctggccgaag gcaaggagcg catttggacg aattcggccg
     2401 gccaatcaat gcgggcggaa ttcgtccgcg aggtggatgg tgaggtcacc ttcttgaaag
     2461 gcggcaagat cgtcagcgtg ccgctcgatt cattgagcga gcgcgaccag cagatcgtac
     2521 gtgacctggc ggctggcaag ccggtttcgg acgatgcacc cgctgcgcct ggcgcatcac
     2581 ccttcagcgg tgaaggtccg atttcagggc cggcgacgcc tggggccgaa gcgaaccgtg
     2641 agccgctgat caaaaagccg attccgattg agaaccgcac gtggaccgac atccacggca
     2701 accagattac cggcaagttc gtgcgaatct tcggatccaa cgtggtgatc caccgcgcca
     2761 gccgctcggt cacggtcaag ttttttgaac tggggagcga agaccaggag tacgtacgcg
     2821 aactgctcac ctcgcgcggg caggaagggt tgattccgcc caaggtggat gtcgaagagg
     2881 gcactggtga tacggctggc ggcgagagcg ctcccgccga agagccgact gcgacagagt
     2941 cgttgccagc cgccgccgca ccggcctcac cgcaggttcc gaggggtcca tcgtttggcc
     3001 caggattccc gggtggtgct ccgcccggcc cgtcgttccc cggtcgtggc ccgggatttc
     3061 cctctcccgg cgccgacccg cccggcgtcg gtgcgggcgg gtacacgccg cctagttaca
     3121 ctcccccaac gcctgctgcg ccatcctcct tcccctccag cgatccctat gcagcacccg
     3181 gcagccgcat cggctccggg tacgcggatg cgcaactgga gcgcatgcgg cagcagaatg
     3241 aagagaacca gcggcgtcag gccgagtcga tgcagcgaat gcaggaccgg atgagcgccc
     3301 agcacgaatc gttcatgaac cgcttcaaga agcaggggga atgcctcaac tgcaagcaca
     3361 gcctgaccga agcggagttc aaccgcacgt cgtgcccgca ctgcggcgtg acatgggact
     3421 acgaggtcga tgagttcggc cacaagcggg agctgcacaa ttcgtcgtac agcagcccgc
     3481 tctcgcggac gaacaacggg gaaccgctga tcgacgcgag gtcggcccgc caggtcggca
     3541 tggtcctggg tatcttcctc ggcctggccg tggtcgtggg catgatcatc ggcgtgattt
     3601 acatcgcgat gaccatcgcc tccgcctcct ccagcagcca gcagcggcac tatcggtagg
     3661 cgctacgaaa gagcacatca gaactcgtgc cactccaaat agacgcacga gacgagcagc
     3721 atcaggccca caaacgccaa tccgctggcg accggccgcc aaggatgcag atcgtcgcgc
     3781 ttctgcgcca gattgccctg gaataagccg aggtcggtgg tccgcttgcc ggtcaaggca
     3841 tacttcaccg tgttgtccag atgccgcggc ttgggcgaga ccaggtgcag cggcttgatg
     3901 ccgtagtagt acgcgagctc ggccgtggac atcgcaggcc gatactcctg ctgccgcgta
     3961 aaagaccgcg cgtcataggc gacgacgcgg ttcgacacat cgccaatcag cagccgctcg
     4021 cccgagaacg caaatccggc gatatcgccc tgccgggaaa tcggcgcgcg gcgggcggtg
     4081 cccgtctcgc tgtcgatcag ccacagcgtg ccgctctgga agaggacgcc gatttgcttg
     4141 ccgccctgcg atgcggccac gtaacgcggc tggacgctgg tgctctccag cgtcagctcg
     4201 tctttcactt tcagat
//
LOCUS       DASUGB010000552         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1450188, whole genome shotgun sequence.
ACCESSION   DASUGB010000552 DASUGB010000000
VERSION     DASUGB010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450188"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFV87_10585"
     CDS             complement(<1..643)
                     /locus_tag="VFV87_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104249.1"
                     /translation="MAGTIDSAAPQTKAQAESPVHAVPWAIVREIGAVLLLVALADVT
                     IYRGAGFAGLALALVAGPIFLLLGSSRPSLKASFWLVVAMILLLAARMIWLGSALAVA
                     VGAILIIAYSVTLQGRRPYVFDLLLGTFQLSIAGGFGLDYYRRCLAGRGPRVSRLLWL
                     SVLLPLGGLAAFGTLFVLANPDLANSIADQATRIYERLVDWYTGMSENWLELGF"
     gene            complement(1066..2049)
                     /locus_tag="VFV87_10590"
     CDS             complement(1066..2049)
                     /locus_tag="VFV87_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5104250.1"
                     /translation="MLAWELHAPHQPLVRADRPIPEPGAGQIVLRVRACGVCRTDLHV
                     VDGELPDPQPLVVPGHEIVGEVTALGARATRFTVGERVGVPWLGQTCRNCRYCTSGRE
                     NLCDDAQFTGYTLDGGYAEYAVADEEYCFRLPRGLDDLHAAPLLCAGLIGFRSLKLAG
                     NALRLGIYGFGAAAHIVAQVASWQGREIYAFTRKGDTEAQQFARDFGAVWAGDSHQCP
                     PNELDAAIIFAPVGALIPAALAAVAKGGTVVCGGIHMTNIPSFEYRLLWGERVLRSVA
                     NLTRADGEEFLAMAPQVPVRTHVQPYSLADANRALDDIRHGQVQGAAILVP"
     gene            complement(2160..>2842)
                     /locus_tag="VFV87_10595"
     CDS             complement(2160..>2842)
                     /locus_tag="VFV87_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104251.1"
                     /translation="AASQGVPLSGGISMETIADEALLTDRGRLVPLYKAQVTGDISGY
                     SEYSEHAFADQRIRRADDDVHANCHGWVFTGGTRLLRGRGVEMILEDNGYHLVKEPAA
                     GDVIVYRDCMNQIIHTGLVRTALDDGLVLVESKWGIEGRYLHRPEDQYYSKTYQYYRS
                     TREPQEASAQARHLVRAVKVLPGSLDSPNQVPTALADGGPMSSIPPLADVNGIPLPKG
                     ETYSVGAE"
BASE COUNT          564 a          923 c          901 g          454 t
ORIGIN      
        1 agaacccgag ttccagccag ttctccgaca tgccggtgta ccagtcaacg agtcgttcgt
       61 agatgcgggt ggcttgatcg gcgatggagt tggccaggtc ggggttcgcc aggacaaaca
      121 gcgtgccgaa cgccgccagg ccgcccagcg gcaagaggac gctgagccac aacaagcgcg
      181 agacccgggg cccgcggccc gccaggcagc ggcgatagta atccaagccg aatccgccgg
      241 cgatcgacag ttggaaggtc ccgagcagca ggtcaaacac gtacggccgc cgaccctgca
      301 gcgtcaccga gtaagcgatg atcaggattg cgccaacggc cacggccaga gcgctgccga
      361 gccagatcat tcgggcggcc aggaggagaa tcatcgcgac gacgagccag aaactggcct
      421 tcaggcttgg ccgcgagctg ccgaggagca ggaagatcgg cccagcgacc agtgcgagcg
      481 ccaggccggc aaaaccagcg ccgcgataga tcgtcacgtc cgccagcgcg acgaggagca
      541 gcactgcgcc gatctcacgc acaatggccc agggaaccgc gtgcacgggg ctctcggctt
      601 gggccttggt ctgcggtgcc gcggagtcaa tcgttccggc cattggaagt ctcacgtgcg
      661 tcggtctcgg acgctaaatc aacagccgtg ctatcgttgg atgcctcttc cacgtcgaaa
      721 cagcaagaaa tggatatgtc aaagggtgct atggtcccag ttccgccgac atcttcccac
      781 gagatggtac tctgtcgcgc ttgataatca gccacgactc gcgccccggt gaaccaggcg
      841 actgcaagaa gtggcggcgc aagggccact acgatcagca atgagcggag acgaaagcgc
      901 atcggggcaa ttccccatca actagtcggc gcgacgatcc aggactgtgc cggcctggcc
      961 acattcagtc tagccggggg caggcgatgc cgcacgagca atattatcat gcaccctgcg
     1021 ccgattccgc cggcttggga acaggcgcgc cgattcggcg aacgatcacg gcacgaggat
     1081 cgcggccccc tgtacctgac cgtggcggat gtcgtccagg gcgcgattgg catcggcaag
     1141 cgaatagggc tgcacgtggg tgcggacggg cacctgcggg gccatcgcca ggaattcctc
     1201 gccgtccgcg cgggtgaggt tggcgacgga ccgcaggacc cgctcgcccc agagcaggcg
     1261 atactcgaac gacgggatgt tcgtcatgtg aatgccgccg cagacgacgg ttccgccttt
     1321 cgccacggcc gccagggcgg cgggaatcaa ggcgccgacc ggcgcgaaga tgatggccgc
     1381 gtcgagctcg ttgggagggc attggtgcga gtcaccggcc cacaccgcac cgaagtcgcg
     1441 ggcgaactgc tgagcctcgg tgtctccctt tcgcgtgaag gcatagatct cgcggccttg
     1501 ccaggaggct acctgcgcga cgatgtgcgc cgccgcgcca aagccgtaga ttcccagccg
     1561 caaggcattg ccggccaact tcaggctgcg aaagccaatc agccccgcac agaggagtgg
     1621 ggcggcgtgc aggtcgtcca gtcctcgcgg caggcggaag caatactcct catctgccac
     1681 tgcgtattcc gcatagccac cgtccagcgt atagccggtg aattgggcgt cgtcgcacag
     1741 gttctcacgt ccgctggtgc agtagcggca gttgcgacag gtctgtccca gccaggggac
     1801 gcccacacgc tcgccgacgg taaaacgcgt cgcccgcgca ccgagggcgg tcacttcgcc
     1861 cacgatttcg tgccccggca caaccaacgg ctgggggtca ggcagttccc cgtccacaac
     1921 gtgcagatca gtccggcaga cgccgcaggc tcgcacacgc agcacgatct gtccggcgcc
     1981 gggctcggga atcggccggt ccgcacggac gagcggctga tgcggcgcgt gcaattccca
     2041 agcgagcacg gaacggcccc catccacagt cgaccgaatc aagaagccgg accctatggt
     2101 atcgtactcg ttcccgtgac cggccgctgc ggtttaatga ctaaccgagc atcccatcgc
     2161 tattcggccc ctaccgagta tgtttcgccc ttgggcaacg ggatgccgtt cacgtcggcc
     2221 agcgggggaa tcgacgacat cggtccacca tcggcaagag cggtcggaac ctggttcggc
     2281 gaatcaagac tgccgggcaa gaccttgacg gcgcgcacga gatgtcgcgc ctgggcgctc
     2341 gcctcctggg gttcccgcgt gctgcgatag tactgatacg tcttcgaata gtattggtcc
     2401 tcggggcgat gcaagtaccg cccttcaatg ccccacttgc tttcgaccag caccagaccg
     2461 tcgtcgagag ccgtccgcac cagaccggtg tggataatct gattcatgca atcgcgatag
     2521 acgattacat cacccgcagc cggctccttg accaggtgat agccgttgtc ctccagaatc
     2581 atttctacgc cgcgaccgcg gagaaggcga gttccgcccg taaagaccca gccgtggcaa
     2641 ttggcgtgga cgtcgtcatc ggcgcgcctg atgcgctgat ccgcgaaggc gtgctccgag
     2701 tactctgagt agcctgaaat atctcccgtg acctgcgcct tgtacagcgg gaccaggcgg
     2761 ccacggtcgg tgagcaaggc ttcatccgca atcgtttcca tcgagatccc accggacaat
     2821 ggcactccct gcgaagcagc ca
//
LOCUS       DASUGB010000553         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_61684,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000553 DASUGB010000000
VERSION     DASUGB010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61684"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFV87_10600"
     CDS             <1..1022
                     /locus_tag="VFV87_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX5104252.1"
                     /translation="SLAFAVPGTILFTCCQVYGRMSAENEVVATKALGVSPLVLLAPA
                     FGLAFVLSVVGVWLNDIAFSWGHVGVQRVILQSVEEIAYGMLRTQKSYSNQRFSIIVK
                     EVRDRKLIRPIMHFQQGGDSPVWTVSAAEAELQSNLELNTLRLIMIDCEVDVAGSRSV
                     LPGRTVQEIPLAFASARGGTSEGVPAHLPLSRIGEETEAQIVAIRELEQSLAAENALA
                     LVTGRFDDLNNSTWNGRRAQLAYARTRLNKLRTEPFRRWAAGFSSLCFVLVGAPFAIQ
                     MKKSDLMSTFGYVFVPILIVYYPFFMYGFDQAKAGALPPYSVWAANLIMVAIGLFLLR
                     RVVRY"
     gene            complement(1042..1272)
                     /locus_tag="VFV87_10605"
     CDS             complement(1042..1272)
                     /locus_tag="VFV87_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104253.1"
                     /translation="MRRFSTALRPALLLALGSNCWLAAEQTASAQPILAQSGMAKTAI
                     GYVLVFLALILALLVVCRPSSRKPAEGDEKKK"
     gene            1601..1945
                     /locus_tag="VFV87_10610"
     CDS             1601..1945
                     /locus_tag="VFV87_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104254.1"
                     /translation="MSKHRSGRSIVGLVLEISVIGLVLTLLPKFGFQQQPASPDQAAP
                     PAANSWWQAAPTSWRQPEVQREVRVEQTLESASQQLLRGVDYATRAAADLAPPPAPAE
                     PLPGQPQEWRRY"
     gene            2023..>2330
                     /locus_tag="VFV87_10615"
     CDS             2023..>2330
                     /locus_tag="VFV87_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104255.1"
                     /translation="MPDPATALEIVSANYPPGPFRCVVFDFDGTLSLLRADWQGLMIP
                     MAVEILAATGTDEPRERLSEIVTEFVTRLTGRPTIVQMQALADEVRRRGQTPDDPLAY
                     L"
BASE COUNT          374 a          738 c          757 g          461 t
ORIGIN      
        1 cctcgctggc cttcgcggtg ccgggaacga tcctcttcac gtgttgccag gtctatgggc
       61 ggatgagtgc cgaaaatgaa gtcgtggcga ctaaggctct gggggtttcg ccgctggtgc
      121 ttctcgcgcc ggcgttcgga ctggcgtttg tcctgagtgt cgtcggcgtg tggctcaacg
      181 acatcgcctt ctcgtggggg cacgtcggcg tgcagcgggt gattctgcaa tcggtcgaag
      241 aaatcgcgta cggcatgctc cgcacgcaga aatcgtattc caaccagcgg ttctcgatca
      301 ttgtgaaaga ggtccgggac cgaaagctca tccggccgat catgcacttc cagcaaggag
      361 gcgattcgcc cgtctggacc gtttccgcag ccgaggccga gctgcaatcg aacctggagc
      421 tgaacacgct gcgactgatt atgatcgact gcgaagtcga tgtggccgga tcgcgatccg
      481 tgctaccggg acggacggtg caagagattc cgctcgcttt tgcttcggcc cgcggcggca
      541 cgagcgaggg cgttccggct catctgcccc tctcacggat cggggaggag accgaggcac
      601 agatcgtggc gattcgggag ctggagcagt cgctggcggc ggagaatgcg ttggcccttg
      661 tcacgggacg attcgacgac ctgaataaca gcacctggaa cggccgccgc gcgcagttgg
      721 cctatgcccg cacgcggctg aataagctcc gcaccgagcc cttccgccgc tgggccgcgg
      781 gctttagttc cctgtgcttc gtgctggtcg gcgcgccgtt cgccattcag atgaaaaagt
      841 cggacctcat gtccacgttc ggctacgtct tcgtgccgat cctgatcgtc tattacccgt
      901 tcttcatgta cggcttcgac caagccaaag ccggcgccct gccgccgtat agcgtctggg
      961 cggcgaatct gatcatggtc gcgatcggac tcttcctgct gcggcgcgtc gtgcggtatt
     1021 gattgggcgt cggcgtcagc gtcacttctt cttctcgtct ccttcagccg gcttgcggct
     1081 gctcggccgg cagacgacga gcagcgctag gatcagggcc agaaagacca ggacgtagcc
     1141 aatggccgtc ttggccatgc cgctttgggc gagtatgggc tgtgccgacg cggtctgctc
     1201 cgcggcgagc cagcagttgg agccgagcgc gagcagcagg gcggggcgaa gagcggttga
     1261 gaatcgtcgc atgaatagtc cgtcaggatt ggaactggct ccattttaaa tgcccgcgcg
     1321 gccgtcgcca gtggttgagt tggcaagcag gaatgtctgc cccacgggtg tgctcgtcct
     1381 gagaaatgtt tgcccgcggt gatacggatt tgccaaacgc gcgttgctag cggccgcgct
     1441 ggttccttca cggaagtcca gcgatgaccg ccacttagag ccctcgcgag gcgagtacgc
     1501 gtgtttgggc accgcaattg cttcctcctt ttttctgcgt ttcaaggatg ccgcccggct
     1561 gatgctcgtc agcgggctgc tcgcaaggaa gggggttctc atgtccaagc accgctcggg
     1621 ccgctcgatc gtcggcctgg ttctggaaat ctccgtcatt ggcctcgtcc tcacgttgct
     1681 gcccaaattc ggctttcagc agcaaccggc gtcgccggac caggccgctc cacccgcagc
     1741 aaattcctgg tggcaagccg ctccgacttc ctggcgtcag ccggaagtgc agcgcgaagt
     1801 tcgcgtcgag cagacgctcg aatcggccag ccagcagctt ctccgcggcg tcgattacgc
     1861 cacccgcgcc gcggccgatc tggccccgcc gcccgccccc gccgagcccc tccccggcca
     1921 gccgcaggag tggaggcgct attagtccgg catccagtcg gaaggtcgcg ccaggtatga
     1981 tggggcttgc cgcagcgtgc cgcctcaatc gtctcgccga atatgcctga ccccgcgacc
     2041 gccttggaaa tcgtcagcgc caactacccc cccggcccgt tccgctgcgt cgtcttcgat
     2101 ttcgacggca cgctctcgct gttgcgggcc gattggcagg ggctgatgat cccgatggcg
     2161 gtcgagattt tggccgcgac ggggacggac gagccgcgcg agcggctgag cgagattgtc
     2221 accgaattcg tgacgcggct gacggggcgg cccacgatcg tgcagatgca ggccctggcc
     2281 gatgaagtcc gccgccgcgg gcaaacgccg gacgatccgc tggcgtatct
//
LOCUS       DASUGB010000554         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_61698,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000554 DASUGB010000000
VERSION     DASUGB010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61698"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFV87_10620"
     CDS             complement(<1..773)
                     /locus_tag="VFV87_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5104256.1"
                     /translation="MSNRKPPPKPEPRTASPLPRPNLARAQQLVLDLMAIPGKSGEEA
                     AVAEFIRGKLLAAGAPADAVFCDNAHRHTPLRGNTGNLILKLPGTLKAPRRMLTAHMD
                     TVPICVGTRPKVDGEFVRSANPATGLGADNRAGCGTILSAALEILEDKLPHPPLTFCW
                     FIEEEVGLHGAHYVQKSLLGKPAMCFNWDGGACDKVTIGATGGYRMQIEIAGLASHAG
                     VCPERGVSAIAIASLAIADLARDGWHGLVIKGKNRGTTNV"
     gene            826..1908
                     /gene="rlmN"
                     /locus_tag="VFV87_10625"
     CDS             826..1908
                     /gene="rlmN"
                     /locus_tag="VFV87_10625"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5104257.1"
                     /translation="MPHLLEPDAWKSLKTWLAERGHKAFRAAQIRKWVFQGRAESFEA
                     MSDLPKGLRAELAAEFSLWSSKVVRHLTSADGTEKLLLELSGGGQIECVLLRDDDRRT
                     ICISTQVGCAMGCVFCASGLDGVERNLTSGEIVEQMLLLQRLLPAEERLSNIVVMGMG
                     EPLANLDNLLPALDEANADDGLGISQRRITISTVGLPKQLHKLCNLNTRYHLAVSLHA
                     PNDTLRNEIVPVNKNIGLAEILAAADRYLHVSNRRLTYEYVLLAGINDRPEHARELAE
                     LFGGRMALLNVIPYNPVAGLPYKTPSANAVNRFRDILLAAGINVKFRQRKGDAINAAC
                     GQLRRSQPPLATIDHEPHPPEAQHAG"
     gene            1898..2458
                     /locus_tag="VFV87_10630"
     CDS             1898..2458
                     /locus_tag="VFV87_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5104258.1"
                     /translation="MPAKVDPQQLQRVELFDNLTTQQAGEILAACTEVLCTAGRVIFE
                     TGQEERALYILLEGTVEVDVASPRAGERQLAQLEQGSVFGESSFFHASPHSATVKALT
                     DAKLLRLDREQFNELLKKDNVAALRVAANAAKILAAKLQEADRCIAGLLEAYAGEKIH
                     EAYSRFRESLGHSFRTPGVKIGFVAD"
     gene            2549..>2955
                     /locus_tag="VFV87_10635"
     CDS             2549..>2955
                     /locus_tag="VFV87_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5104259.1"
                     /translation="MKTSGLIVALLAAAPLAGAERPNLLIVTVDDMSADSIGAFGCKL
                     AGTSPHVDQLAKEGLRFHHAHVQVGNCMPSRNVMWSGRYPHNNRVEGFYQVKDPGYPV
                     LVDLLKEAGYFTGIRGKVSHSTPYSPYAWDVVL"
BASE COUNT          520 a          939 c          975 g          521 t
ORIGIN      
        1 acgttcgtcg tgccgcggtt cttgcctttg atcaccaggc catgccagcc gtcgcgggcc
       61 aggtcggcga tggccagcga ggcgatcgcg atggcgctca cgccccgctc ggggcagacc
      121 ccggcgtggc tggcgaggcc cgcaatctcg atctgcattc ggtagccgcc cgtcgccccg
      181 atcgtcacct tgtcgcaggc cccgccgtcc cagttgaagc acatcgccgg cttgccgagg
      241 agcgatttct gcacatagtg cgccccgtgc aggccgacct cctcttcgat aaaccagcag
      301 aacgtcagcg ggggatgcgg cagcttgtcc tccaggattt cgagggccgc ggaaagaatc
      361 gtcccgcagc cggcacggtt gtcggctccc agacccgtcg ccggattggc cgagcggacg
      421 aactcgccat ccaccttcgg cctggtgccg acgcagatcg gcacggtgtc catgtgggcg
      481 gtcagcatcc gccgcggagc cttcagggtg ccgggcagct tgaggatcag gttgcccgtg
      541 ttccccctga gcggcgtgtg ccggtgggcg ttgtcgcaaa aaactgcgtc cgcgggcgct
      601 ccggctgcca acagcttgcc gcggatgaac tccgccacag ccgcttcttc gccgcttttg
      661 ccggggatgg ccatcaggtc gaggacgagc tgctgggcgc gggcgaggtt gggacggggc
      721 agggggctcg cggttcgggg ctcgggtttc ggaggaggtt tgcgattgct catttggggg
      781 tggcttcgct gagacggagt acgaggcggg ctgtatgata cagcaatgcc ccacttgctc
      841 gaacctgacg cctggaaatc cctgaaaacg tggctcgccg agcgcggcca caaggcgttt
      901 cgcgctgcgc agatccgcaa gtgggtcttt caagggcggg ccgaaagctt tgaggcgatg
      961 agcgacctgc cgaaagggtt gcgagccgag ctggcggcgg aattctccct ctggtcgagc
     1021 aaggtcgtgc ggcacctgac gtcggccgat gggacggaga agctgctgct ggagctctct
     1081 ggcggcgggc agatcgagtg tgttctgctg cgcgacgacg accgccgcac gatctgcatc
     1141 agcacgcagg tcggctgcgc gatgggctgc gtcttttgtg ccagcggcct ggatggcgtt
     1201 gagcgcaacc tgaccagcgg cgagattgtc gagcagatgc tgcttttaca acgcttgttg
     1261 ccggccgagg agcggctttc caacatcgtt gtgatgggca tgggcgagcc gctggcaaat
     1321 ctcgacaatc tgctgccggc gctggacgag gcgaacgccg acgacgggct gggcatcagc
     1381 cagcgtagga ttacgatcag cacggtcggc ctgcccaagc agcttcacaa gctgtgcaac
     1441 ttgaatactc gctaccacct cgctgtctcg ctccacgcgc cgaacgacac gctccgcaac
     1501 gagatcgtcc cggtcaacaa gaacatcggc ctggccgaga tcctggccgc ggccgaccgg
     1561 tatcttcatg tgtccaatcg ccggctgacg tacgaatatg tgctgctggc ggggatcaac
     1621 gaccggcccg agcacgcccg cgagttggcc gagctgttcg gcggacgaat ggccctgctc
     1681 aacgtgatcc cttataatcc ggtcgctggg ctgccttaca agactccgtc cgcgaatgcg
     1741 gtgaaccggt tccgcgacat cctgctggca gccggcatca acgtgaaatt ccgccagcgg
     1801 aaaggggacg cgatcaacgc ggcctgcggg cagcttcgcc gcagccagcc gccgctggcc
     1861 accatcgacc acgagccaca tcctccggag gcccaacatg ccggctaaag ttgacccgca
     1921 gcagttgcag cgcgtcgagc tgttcgacaa cctcacgacc cagcaggcgg gcgaaatcct
     1981 cgcggcttgc accgaggtgc tctgcacggc cggccgcgtg attttcgaga ccgggcagga
     2041 agagcgggca ttgtacattc tgctggaggg gacggtcgag gtcgatgtcg cctcgccgcg
     2101 ggccggcgag cggcagctcg cccagctcga acaggggagc gtcttcggcg aaagcagctt
     2161 ctttcacgcc agcccgcaca gcgccacggt gaaggccctg accgacgcga agctgctgcg
     2221 gctggaccgc gagcagttca atgagctgct gaagaaagac aacgtcgccg ccctccgcgt
     2281 ggccgccaac gccgcgaaaa tcctcgccgc caagctgcag gaagccgacc gctgcatcgc
     2341 cggcctcctg gaagcgtatg ccggcgaaaa gatccacgaa gcctacagcc gcttccgcga
     2401 atcgctcggc cactcgttcc ggacgccagg ggtcaaaatc ggcttcgtcg ccgattagca
     2461 ggcgcgagtc ggaaaaaaat ctgcgaatct ttctgcggcc agacattgga tccgctgagc
     2521 tgcgcatcga tggagcggag gtccaaccat gaagacttct ggattgattg ttgccttgtt
     2581 ggccgccgct ccactcgccg gtgccgagcg gccgaatctg ctcatcgtta cggtggatga
     2641 catgagcgcc gattcgatcg gggcgttcgg ctgcaaactg gcgggcacgt cgccgcacgt
     2701 cgatcagttg gccaaggaag ggctgcggtt ccaccatgct cacgtgcagg tcggcaactg
     2761 catgccgtcg cggaacgtca tgtggtcggg ccgttacccg cacaacaacc gcgtcgaggg
     2821 cttttatcag gtcaaagatc ccggttatcc ggtgctcgtg gacttgctga aggaagcagg
     2881 ctacttcacg ggcatccgcg gcaaggtgag ccattcgacg ccctactcgc cctatgcttg
     2941 ggacgtcgtg ctcga
//
LOCUS       DASUGB010000555         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1312336, whole genome shotgun sequence.
ACCESSION   DASUGB010000555 DASUGB010000000
VERSION     DASUGB010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312336"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFV87_10640"
     CDS             complement(<1..394)
                     /locus_tag="VFV87_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104260.1"
                     /translation="MRKAQARKHLFEALEERQMLAGDLVYEADNAAPLTLRLSGGTVE
                     IINSNTSGVLASEALSDITTGVRIEGNEFDVRLTIDDSVPIIPGGVLFVGGTGNSTLV
                     GPEVATDWDLTGAGAGTVTFSTGVITFQD"
     gene            complement(1606..1827)
                     /locus_tag="VFV87_10645"
     CDS             complement(1606..1827)
                     /locus_tag="VFV87_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104261.1"
                     /translation="MTRLRDTCGFWLLGIRPKDVQWGVTSAPMIDGQGKGTILNDDGV
                     ATGGNKTSAAAAVDAAIEQLIFSSPMKRG"
     gene            complement(1851..4262)
                     /locus_tag="VFV87_10650"
     CDS             complement(1851..4262)
                     /locus_tag="VFV87_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5104262.1"
                     /translation="MFLLHLRSSLAVLPTLPMALAMSFTAMYWFGVDSNIMSLAGIAI
                     AIGDVADMGIIMTENIYRRLAAEPDRPHAEVVYEGATEVGSAIVTAVSNTIVSFIPVF
                     ALTDQEGKLFKPLAYTKTFAITASVILALTLVPVLCHYLLRPTQWPRRRSLVLGVLIG
                     TSAAIVTYLVTSWQLQTASVWRGWPMALGVGVMVGAAVYRMGREQLLPLEENIVARTI
                     NGVYRPCITWVLDHKLVFLTIPTAITLAGLVIWLGFARVAYPLQASLNAVEVHPESAR
                     WWRGLEQRLPGIGREFMPPLDEGSFLYMPSLLPAASLTEVQKALAAQDLAIASVPEVA
                     SVVGKAGRAETALDPAPIGMIETIILLKPESEWRMVADGRGGQRRITKDEILAELQTK
                     TNIPGVLPTWLQPIQTRIVMLQTGFRAMMGVKVFGADPQVIEQIGLQMEQILREVPGA
                     TDVVADRIVGKPYIEYEIDREAMARYGVNIRDVQDVIEIAIGGENLTTSVEGRERYPI
                     RVRYLRELRERFDDLERILVPTSDGAHVPIGQLATIRYSLGPQELKSENGLLVGYVTM
                     NTRDRDEVSVVEDAERLLQAEKRKSDELIAAGRHSEARLVVPPGYYWKWSGQFENQQR
                     ATRRLSWVVPLVLLAMFVLLFVGLGRWWLVLIVFFGIVVSASGGFLMLYLWGANLSVA
                     VWVGFIALFGVTDDSSVVMLDFLEKQFKDRTPSSISEIRAWVTEASLKRVRPLMMSTA
                     TTVVGLMPIFLTHGRGSDVMQPMAIPSVGGMAINLITLFIAPCVYGLVKEWQFRRHFD
                     SQLQI"
BASE COUNT          860 a         1326 c         1250 g          828 t
ORIGIN      
        1 catcctggaa cgtgatcaca ccggtggaga atgtcacggt gcccgcaccg gcgcccgtga
       61 ggtcccagtc ggtagcaacc tccggcccca ccagcgtgct gttgccggtt cctcccacga
      121 acagaacgcc cccagggatg atgggcacgc tgtcatcgat cgtgagccgc acgtcgaatt
      181 catttccctc aattcgtacg ccggttgtga tgtccgagag ggcctcggaa gcgagaaccc
      241 ccgacgtgtt cgaattgatg atttcgaccg tcccgccgga cagccgcagc gtcaggggcg
      301 cggcgttgtc ggcctcataa acgaggtctc ccgccagcat ctgccgctct tcgagcgcct
      361 cgaacaggtg ttttcgcgcc tgagcctttc gcaaatcaga gtgccgaggg gacgaaaaga
      421 gccttcgaat ccaggggtgc atcttcattg cgtggttatg tcgttaaggc gcactgcacc
      481 taggcaagcc cagccagttg ccgtgtttgg cttgccaaca gaaggaggaa ggaatatgag
      541 ttgtccaagt cgtacgcaac cctgtccgcg tcacggccag ggaaactctc gttactggca
      601 accgccctgt caacaaccag tgccgatttt ttggcgtgaa tctcgtgtta ggcgcgctcc
      661 tgggaattcc gaattcacgc tcatgcttgg agtagccggt cgcttggtac cccaaaatcc
      721 atgcgcgctc cgccagcgct cgactacgcc ggcagtatct agaccaattc aaaccggtaa
      781 ttaaactagt tttgagccgt tagaagtagg gcgagtctct agttgtgcgc tgatttgagt
      841 cggtccgccg aaatctcctg gccggtgtag agcggcgggg caactcaacg tgattcttcg
      901 gcggcggccg ccaccggtac tggctcaacc gcactatcca cgctttccca gcggccgccc
      961 ctgtcctaat acaagtccgt tagcgcacgt tcccactgtt ctcgagagcg agaggtcgga
     1021 aatggaacgg ggcgaacggg ctcggccgac gtccaaacaa cttctggtct accctcgcta
     1081 tcaatgcggg cgatccgcgc ggtcctccag gcatgctgcg attccggatt gacgaaaacc
     1141 tacgcctcgg gggcatcgaa gcgctggcca gcgatggccc gacggatcgc cgaagcctcc
     1201 gtgctgccgg ctgccctcac cgcttgggcc cagagatgga ctttaacata cgaggcttcc
     1261 agcggatccg acaagggccg ctgtggaccg aacttttcgc ggaagcgcga cacaaacgcc
     1321 cggtttgccg ttgaacggtg tgaccgcctt gcgactctcc gggtgcaaac tcgcagcggt
     1381 tgcatcggcc acgcgcgcaa cgaagatctt gccagttgca gcggtgtctc atcccgcagg
     1441 acaccgccgc aggttcgtgc aggccgtaac tcgcgcttgt tggtccaacc gccagcagtt
     1501 tggagattct cgatttgtcg gcggtcgaaa tgaccgccga gctttctttg ggcacggcgg
     1561 accaaaggat ttccagactc ccggggaagt ggtcgcgttc gttccctagc cccgcttcat
     1621 ggggctggag aaaatcaact gctcgatcgc cgcatcgacg gctgcagcag cggaggtctt
     1681 gttgccgccg gtggccacgc cgtcgtcgtt gaggatcgtc cctttcccct ggccgtcgat
     1741 catcggcgcg ctggtcactc cccactgcac atccttcggt ctaattccaa gcagccaaaa
     1801 gccgcaagta tcccgaagac gagtcatcgt ttcagagcaa tggtacggtt tcagatttgc
     1861 agctgcgaat cgaaatgcct tcgaaactgc cattccttaa ccaggccata cacgcaaggg
     1921 gcgatgaaga gcgtgatcag gttgatcgcc atcccgccga cgctgggaat cgccattggt
     1981 tgcatcacgt cggagccgcg gccgtgcgtg agaaagatcg gcatcaggcc aacgaccgtc
     2041 gtggctgtgg acatcatcaa cggccggacg cgcttcaggc tggcttcggt gacccatgct
     2101 cgaatctcgc taatactgct aggcgtgcgg tctttgaatt gcttctcgag aaaatcgagc
     2161 atcacaacac tgctgtcatc ggtcactccg aacagggcaa tgaatccaac ccacaccgcc
     2221 acactcaaat tcgcacccca caaatagagc atgagaaacc cacccgatgc cgagacgaca
     2281 attccgaaga agacaatgag cactagccac cagcggccga ggcctacaaa tagaagcaca
     2341 aacatcgcca gcagcaccag cggcacgacc cacgacaggc gtcgcgtagc acgctgttga
     2401 ttctcgaact ggcccgacca tttccaatag tagcccggcg ggacgaccag ccgcgcttcc
     2461 gaatggcggc cggctgcaat cagctcatcg cttttgcgtt tttccgcttg cagcaatcgc
     2521 tcggcgtctt ccacaacact cacttcatcg cgatcgcgcg tgttcatcgt gacatagccg
     2581 actaacaggc cgttctcgct cttcaattcc tggggaccga gactgtaacg gatcgtcgcc
     2641 agttgcccga tgggcacgtg cgcgccatcg gaggtcggca cgaggatgcg ctccaagtcg
     2701 tcgaaccgct cgcgcagctc gcggaggtag cggacgcgga tcgggtatcg ctcgcgccct
     2761 tccacggaag tcgtcaggtt ctcgccgccg atggcgatct cgatcacatc ctgcacgtcg
     2821 cggatgttga caccgtagcg ggccatcgct tcgcggtcga tctcatattc gatgtacggc
     2881 ttgcccacga tgcggtcggc gaccacatcg gttgcgccgg ggacttcgcg caggatttgc
     2941 tccatctgca ggccaatctg ctcgatgacc tgcgggtcgg cgccaaagac cttgaccccc
     3001 atcatggcgc ggaacccggt ttgcagcatg acgatccgcg tctgaatcgg ctgcagccag
     3061 gtcggcaaga cgccgggaat gttggtcttg gtttgcagtt cggccaggat ctcgtccttg
     3121 gtgatgcggc gctgcccgcc gcggccgtct gcgaccattc gccactcgct ttcgggcttg
     3181 agcaggatga tcgtctcgat catcccgatg ggggcggggt ccagggccgt ttcggcacgc
     3241 ccggcctttc ccacgacgct ggccacctcg ggaaccgacg cgatggccag atcttgcgct
     3301 gccagggcct tttggacttc tgtcagggag gccgccggca gcagcgatgg catgtaaagg
     3361 aatgagcctt cgtcgagtgg cggcatgaac tcgcggccaa tccccggcag acgctgctca
     3421 agtcctctcc accagcgggc cgattccggg tgaacttcaa ccgcgttcaa gctggcctgc
     3481 aggggatacg cgactcgagc aaatccgagc caaatcacca gaccggccag cgtaatcgcc
     3541 gtgggaatcg tcaagaacac cagcttgtga tcgagcaccc aggtgatgca gggccgatag
     3601 acgccgttga tcgtccgggc gacgatgttt tcttcgagag gcaaaagttg ctcgcggccc
     3661 atgcgataga cggccgctcc gaccatgacg ccgacgccga gagccatggg ccaccctctc
     3721 cagacgctgg ccgtctgaag ctgccaactc gtcacgagat aggtaacgat cgccgcgctt
     3781 gtgccgatga gtactcccag aacgagcgac cgtcttctgg gccattgcgt cggcctcagc
     3841 aggtagtgac aaaggacagg cacgagcgtc agggccagga tcaccgaagc ggtgatggcg
     3901 aacgtcttgg tataggccaa gggcttgaac agctttcctt cttgatccgt caaagcgaat
     3961 accgggatga acgagacgat cgtgttggaa accgcggtga caatggcgct gccgacttcc
     4021 gtggcacctt catagacgac ctcggcatgc gggcgatccg gttcggcagc taggcggcgg
     4081 taaatgttct ccgtcatgat gatgcccatg tcggccacgt cgccgatggc aatcgcgatt
     4141 ccggccagcg acatgatgtt gctatccacg ccaaaccaat acatcgcagt gaagctcatg
     4201 gccagcgcca tgggcagcgt tggtaataca gcgagcgaac tgcgaaggtg gagcagaaac
     4261 agaa
//
LOCUS       DASUGB010000556         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_480458, whole genome shotgun sequence.
ACCESSION   DASUGB010000556 DASUGB010000000
VERSION     DASUGB010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480458"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFV87_10655"
     CDS             complement(<1..490)
                     /locus_tag="VFV87_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5104263.1"
                     /translation="MAATDMASTPPRTSQSNDGQTSPRTATLQALANGINRLEAIGRG
                     DPIEAVIHKKVLDEEPIIQVENFSLWYSHKQALFDISMPISKGKVTAIIGPSGCGKST
                     LLRCVNRMNDLIEGVRTQGDMKVSGDSIFAKGVDVIELRKRMGMVFQKPNPFPMSIFE
                     NVV"
     gene            779..1708
                     /locus_tag="VFV87_10660"
     CDS             779..1708
                     /locus_tag="VFV87_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5104264.1"
                     /translation="MNASFPSRREFLATSAGLAAAGLLARGALAAESASAPFKISLAQ
                     WSLHKTIFGGQLDNRDFARAAKEDYGIEAIEYVNQFWKDKAKDAAYVAELKKRAEDLG
                     VKTLLIMCDGEGALGDSDDKKRTQAVENHYKWVEAAKELGCHSIRVNAQSNKMLPYEE
                     QQKLAADGLRRLSEFAAPHGLNVIVENHGGLSSNGQWLAGVMKLVDRKNCGTLPDFGN
                     FNLGDGKEYDRYLGVDELMPFAKAVSAKSHDFDAEGNETHTDYVKMLEIVVKKHDYHG
                     YCGIEYEGSKLSEPEGILATKKLLERVREKLAG"
     gene            1743..2687
                     /locus_tag="VFV87_10665"
     CDS             1743..2687
                     /locus_tag="VFV87_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104265.1"
                     /translation="MRRPSIISIVLALAIVSRAGAQPGTQQSGALQCVARANGNWIEI
                     AATVMKPVVETKTVTVVNGQGQPGNRTISETRLATESVIVRCAAGEVRAYTAARQPLT
                     TDELLQRLEKETVVFVCSSQRKADAAYASLLKPQTICLFLPTESSGLPDGATLPAENL
                     PPLKALPGKLQPTAGLATITKDGQCVVRQQFVLRADQTAMRTVESAEGSRQAPYSIKQ
                     QFTTESLLEFPASEAHLLDQSGQRLDAADAAAALANEIPVLIATDGRPVDPAFLTIVR
                     PEALVLVLPTLLTPAGQEPAAPLPASPAVRPGAAESAR"
     gene            2696..3478
                     /locus_tag="VFV87_10670"
     CDS             2696..3478
                     /locus_tag="VFV87_10670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5104266.1"
                     /translation="MFHPQGPTFWELAEQCLSSTERGYDLLAPKFDYTPFRTPDILLD
                     GMAKVLGEPQSIDAALDLCTGTGAAIRILRPLVRAAEPAGNALRGDPERHGGRSLQRV
                     VGIDFSPGMLEVARQSFPNEPNQPPVDFVLGDVLEMDFREEFDVATIAGAQGHILPKD
                     EPRFLERIRAALKPGGRFVFLTTTMPPVWSPRWVLSRGFNAAMHVRNAIKRPSFIMFY
                     LTFLLPDCLKLLDEQGFDAQVHEDVFADRWRPIQVVEATRRP"
BASE COUNT          675 a         1060 c         1245 g          665 t
ORIGIN      
        1 agacgacgtt ttcgaaaatg ctcatcggga aaggattggg cttttgaaag accattccca
       61 tccgcttgcg gagctcgatg acatcgaccc ctttggcgaa gatcgaatcg ccgctgactt
      121 tcatgtcgcc ttgcgttcgc accccttcga tcaggtcgtt catccggttc acgcaccgca
      181 gcagggtcga tttgccgcag ccggacgggc cgatgatcgc cgtcaccttc cctttggaga
      241 tcggcatgga gatatcgaag agggcctgct tgtggctgta ccagagcgag aagttctcga
      301 cctggatgat cggctcttcg tccaggacct ttttgtggat gaccgcttcg atcgggtcgc
      361 cgcggccgat cgcttccagc cggttgattc cgttggccag ggcctggagc gtggccgtac
      421 gaggcgaggt ttgcccgtcg ttggattggc tggtgcgggg cggtgtagag gccatgtcgg
      481 tcgcggccat gagagcgctc gcgggtgctg gttgtatcgt aaagtccgag cgtgccgagc
      541 gggagggcga agcgggttaa gaattcgggt agattggcga gcgccgtgcg gtgtcccccg
      601 cggccgcgcg tccggcgcgg caactatact gagatttcgt ttaacggcga gccgcaggcg
      661 atgccatttg ccgcatcttt gcggcgggcg gctacgcctc cgcttcgctg cggctacgcg
      721 ttaaacgaga ccttgatgat aataggcgaa atcctggctt tatctttgga atccgaacat
      781 gaacgcatcg ttcccttcac gtcgtgagtt tctcgcaact tccgcgggac tggccgctgc
      841 gggccttctg gctcgcgggg cgttggctgc cgaatcggct agcgcgccct tcaagatttc
      901 gctggcccag tggtcgctgc acaagacgat cttcggcggc cagctcgaca accgcgactt
      961 cgcgcgggcg gcgaaagaag attacggcat cgaggcgatc gagtacgtca accagttctg
     1021 gaaggacaag gcgaaggacg ctgcctatgt ggccgagctc aagaagcggg ccgaggattt
     1081 gggggtcaag acgctgctga tcatgtgcga cggcgaaggg gcgctgggtg attccgacga
     1141 caagaagcgc acgcaagcgg tcgagaacca ctacaagtgg gtcgaagccg ccaaagaact
     1201 cggctgccat tcgatccgcg tcaatgccca gtcgaacaag atgctgcctt atgaagagca
     1261 gcagaagctg gcggcggatg gcctgcggcg cttgagcgag tttgcggccc cgcacggcct
     1321 gaatgtgatt gtggagaatc acggcggcct ctcgagcaac ggccagtggc tggcgggcgt
     1381 gatgaagctc gtcgatcgga agaattgcgg cacgctgcca gacttcggca attttaatct
     1441 cggcgacggc aaagagtacg atcgctacct gggcgtcgat gagctgatgc cgtttgccaa
     1501 agccgtcagc gccaagagcc acgacttcga cgccgagggg aacgagacgc acaccgacta
     1561 cgtcaagatg ctggagatcg tcgtgaagaa gcacgactat cacggctact gcggcattga
     1621 gtacgaaggg agcaagctga gcgagcccga ggggattctc gcgacgaaga agctgctgga
     1681 gcgagtgcgg gagaagctgg cggggtagcc tggacgcgga gcattcaacg agagaggcgc
     1741 ttgtgagacg accgtcgatc atcagtatcg tgcttgccct ggcaatcgtt tcgcgcgccg
     1801 gcgcgcagcc gggcacgcag cagagcggag cgctgcagtg cgtggcgcgt gccaacggga
     1861 actggattga gattgctgcg acggtcatga agcctgtcgt ggagaccaag acggtcacgg
     1921 tcgtcaacgg gcaaggacag ccgggtaacc ggacgatcag cgagacgcgg ctggccacgg
     1981 agtcggtgat cgtgcggtgt gcggccggcg aagtacgggc ctataccgcc gcacgtcagc
     2041 cactaacgac agacgaattg ctgcagcggc tcgagaaaga aacagtcgtg ttcgtgtgca
     2101 gctcgcagcg gaaggcggac gcggcgtatg cgtccctcct caagccccag accatctgcc
     2161 tgttcctgcc caccgagtcg agcgggctgc cggacggcgc cacacttcct gcggagaatc
     2221 tgccgccgct gaaggccctc ccgggcaaat tgcagccgac cgcgggcctg gccacgatca
     2281 ccaaggacgg gcaatgcgtc gtgcggcagc aattcgtact gcgagcggat caaacggcga
     2341 tgcgcaccgt cgaatcggcc gagggctcgc ggcaggcgcc gtattcgatc aagcaacagt
     2401 tcacaaccga gagcctgctg gagtttcccg cgagcgaagc ccacttgttg gaccagagcg
     2461 gccagcggct ggacgctgcc gacgcggcgg cggcgctggc gaacgagata cccgtgctga
     2521 tcgccaccga cggccggccg gtcgatccgg cgttcctgac gatcgtccgc ccggaggcgc
     2581 tggtgctggt gttgccgacg ctgttgaccc cagcgggaca ggagccggcc gcgccacttc
     2641 ccgccagtcc ggctgtcagg ccaggggctg cggaatcggc tcggtaagga gatccatgtt
     2701 ccatccgcag gggccgacgt tttgggaact ggccgagcag tgcctgtcat cgactgagcg
     2761 ggggtacgac ttgctggccc cgaagttcga ttacacgccg ttccgcacgc cggacatcct
     2821 gctggatggg atggcaaagg ttctgggcga gccgcagtcg atcgacgcgg cactggatct
     2881 ctgcacgggg accggcgcgg cgattcggat tctgcggccg ctcgtgcgtg cggctgagcc
     2941 tgcagggaac gccctccgtg gcgatccgga acgccacgga gggcgttccc tacagcgcgt
     3001 cgtgggaatt gacttcagtc ccggcatgct ggaggtcgcc aggcagagtt ttcctaacga
     3061 gccgaatcag ccgccggtcg atttcgtcct gggtgacgtg ctggaaatgg acttccgcga
     3121 ggaattcgac gtggcgacga tcgccggagc gcagggacac atccttccga aggatgagcc
     3181 gcggttttta gagcggattc gcgccgccct caagccgggc gggcggtttg tgttcctgac
     3241 gacgacgatg ccgccggtgt ggtcgccgcg gtgggtcctt tcgcgcgggt tcaacgcggc
     3301 gatgcacgtg cggaacgcga tcaagcggcc gagcttcatc atgttttacc tgacgttcct
     3361 gctgccagat tgcctcaaac tgctggatga gcagggattc gacgcccaag tgcacgagga
     3421 cgtcttcgcc gacagatggc ggccgatcca ggtggtcgag gcgacgcggc ggccgtgagt
     3481 ttgcgttgca ccccctgcgg cgcgcgtgta ctcttatcca ttgacgtttg cccacggatt
     3541 cgcaggagcc actgatgagg gaatgcaaag ttcgcgatca tgcgaactgc catgcgttca
     3601 cgcacttcaa tgcgcgtgag gcggagggcg tgcgcctggt cagcc
//
LOCUS       DASUGB010000557         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1592290, whole genome shotgun sequence.
ACCESSION   DASUGB010000557 DASUGB010000000
VERSION     DASUGB010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592290"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VFV87_10675"
     CDS             complement(<1..1237)
                     /locus_tag="VFV87_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5104267.1"
                     /translation="MFRRRMPGLPQTKTFDLLESLLADRILILDGAMGTMIQRYKLSE
                     ADVRAERFRGHHKDLVRFSDILCLTHPEKITEIHRQYFAAGADIVETNTFGASFVGME
                     EFELPRELVREINTAAVRCARTAADEWNERTPHQPRFVAGSIGPTAKQMAISTRVDDP
                     AWRGTDFDAMVDSYYEQVEALVDAGVDLLLPETFIDTLNLKACLFAIQKLCEARGVQI
                     PVMASGTFGPGGVTFVSGQSVEAFWDSLANVPLLTVGMNCALGPETMRPQIEELARVA
                     TARIHAYPNAGLPNEMGQYDLGPAKMAELVGEWAENGWMNIIGGCCGTTPEHIAAIAE
                     RVRRAKPHHSAEQPHWLRLSGTLPFTQRPDSNFIMVGERTNVTGSKAFARLIRDGKFE
                     EAVEVARQQVAGGATIIDVN"
     gene            1518..>2874
                     /locus_tag="VFV87_10680"
     CDS             1518..>2874
                     /locus_tag="VFV87_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104268.1"
                     /translation="MTATLSVEPRRTGKRGAPPTRELPVLFHLMDVSSTVSRPAATIL
                     EPPPDPPAASKPPDTPPTVAAYSLPEPAMNFQPPPSPFPPPLPEPPRIVERPAVTAIV
                     EASPPIELPPTEPPAIEPLSLDLPATMEVAIARPETPIEAREPQMAEQPPIESAPDFS
                     SAIREARAAEVEANSRSVGEESACGNADSIFEETLETAPLPAVEPGTVTDSTPPGEPQ
                     ASTAKKARPRVRDTWFAAHGKYIAIGFVLALIGTILLARFGRRAPQPVADALQHSHPG
                     DAGEPFDISLPKPVTVVDEAAEDKQSGGPADGTAESSPAKLTEQPADEPKPQTDLHPP
                     TIPQLASEPSDSNQPPHDNLFPWGQPNENRLATRPEPTLQPPANSPRAQPQYPVTEQS
                     AGVPPGFPVTNSPYVEPPQAGVPPAINGGRGGQFVPPPETQPGPQWQPGPPPPGFATD
                     YR"
BASE COUNT          535 a          983 c          895 g          461 t
ORIGIN      
        1 tgttcacgtc gataatcgtc gccccgccgg ccacctgctg gcgggcgact tcgactgctt
       61 cctcgaactt gccatcgcgg atcaatcggg cgaaggcctt gctgccggtg acgttcgtcc
      121 gctcgccgac catgatgaaa ttgctgtcgg gacgctgcgt gaacggcagc gtgcctgata
      181 gccgcagcca gtggggctgc tcggccgaat ggtgcggttt ggcgcgacgg acacgctcag
      241 cgatcgcggc gatgtgctcg ggcgtcgtcc cgcagcagcc gccgataatg ttcatccagc
      301 cgttttccgc ccactcgccg acgagctcgg ccatcttcgc cgggccgagg tcgtattgcc
      361 ccatttcgtt cggcaggccg gcgttgggat aggcgtgaat gcgggcggtc gccacgcggg
      421 ccagctcttc gatctgcggg cgcatcgtct ccggaccgag ggcgcagttc atgccgaccg
      481 ttagcagcgg cacgttggcc agcgaatccc agaaggcctc gaccgactgc ccgctgacga
      541 acgtcacgcc gccggggccg aacgtgccgc tggccatcac gggaatctgc acgccgcggg
      601 cctcgcacag cttctggatc gcgaacaggc acgccttgag gttgagcgtg tcaatgaatg
      661 tttccggcag cagcagatcg acgcccgcat cgaccagggc ctcgacctgt tcgtagtacg
      721 aatcgaccat cgcgtcgaag tcggttccgc gccaggccgg gtcatcgacg cgcgtcgaaa
      781 tggccatctg cttggccgtc gggccgatcg agccggccac aaaccgcggc tgatgcggcg
      841 tgcgctcgtt ccactcgtcc gcagccgtgc gggcacagcg gacggcagcg gtgttgatct
      901 cgcggaccaa ctcccgcggc agctcaaact cctccatgcc gacgaagctg gcgccgaacg
      961 tattggtctc gacgatgtcc gccccggccg cgaaatactg ccggtgaatc tcggtgatct
     1021 tctccggatg cgtcaggcag aggatatcgg agaagcgaac caggtccttg tgatgcccgc
     1081 ggaaccgctc ggcccgcacg tccgcctcgc tcagcttgta gcgctggatc atcgtcccca
     1141 tcgcgccgtc caagatcagg atccggtcgg ccaggagcga ttcgagcagg tcgaaagtct
     1201 ttgtttgagg caggccaggc atacgtcgcc gaaacatgga gggaatccct aactatccta
     1261 gacatccggg tttaccgcaa tgcggatagt tgttgaaaca ataatttgtc cccggcaaag
     1321 ggagcgggtg caaatcggaa aggttcgcct gctggtcggc caaaggccgc aaagtttgcc
     1381 cagttctcaa gatcgacaca acccctaccg ataaagggca agcgggatcg agcgcgcgcc
     1441 gcacggacgc ggcggttaga ccgctgggcc cttcgcccga atggggaggg cgaacgaagc
     1501 tgggaaagga cgccctgatg actgcgacac tctcggtcga gccgcgccgc acgggcaaaa
     1561 gaggggcccc tcccacgcgc gagctgccgg tgctgttcca cttgatggat gtcagcagca
     1621 ccgtgtcgcg gccggcagcg acgattctcg aaccgccgcc cgatcctccg gccgccagca
     1681 agccgcccga tacgccgccg acggttgcgg cgtattcgct gcctgagccg gccatgaatt
     1741 tccaaccgcc gccttcgccc ttcccgccgc cactgccaga gccgccccgc atcgttgaga
     1801 ggcccgcggt cacggcgatc gtcgaagcat caccgccgat cgaactgcca ccgaccgaac
     1861 cacctgcgat cgagccgctg agtcttgatc tgcctgcgac aatggaagtc gccatcgcca
     1921 ggccagaaac gccgattgag gcgcgcgagc cgcagatggc cgagcagccg cccattgaat
     1981 ccgcgcctga ctttagcagc gcaattcgtg aagctcgcgc agcggaagtc gaagcgaatt
     2041 cgcgcagcgt gggtgaagag tcggcttgtg gcaatgcaga ctccatcttc gaagagactc
     2101 tggaaacggc cccattgccg gctgtagagc ccggcacggt tacggacagc acgccccccg
     2161 gagagccgca agccagcacg gcaaagaaag cccggccgcg agtacgcgac acgtggttcg
     2221 cggcgcacgg taaatacatc gccattggtt tcgttttggc gctcattggc acgattctcc
     2281 tcgctcgctt tggccgcaga gccccacagc cggtcgccga cgccctgcag cacagccacc
     2341 cgggcgacgc aggcgagccg tttgatatca gcctccccaa gcccgtgacc gtcgtggacg
     2401 aagcggccga ggacaagcaa tccggaggcc cggccgatgg aactgcggaa tcgagcccgg
     2461 cgaagcttac ggaacaaccg gccgacgagc ccaagcccca gaccgacttg catcccccga
     2521 ccattcctca actcgcaagc gaaccgtcgg actccaacca gccgcctcat gacaacttgt
     2581 ttccttgggg ccagccgaac gagaaccgcc tcgcgacgcg tccggagccg acattgcagc
     2641 cgccggccaa ttcgccgcgg gctcagccgc agtatcctgt taccgagcaa agcgccggcg
     2701 tgccgccggg ctttcccgtg acaaattcgc cgtacgtcga accaccgcag gcgggcgtcc
     2761 cgccagcgat caacggcggt cgcggcggac aattcgtgcc gccgcccgaa acccagccgg
     2821 gaccgcagtg gcagccgggt ccgccgccgc caggctttgc gaccgactat cgca
//
LOCUS       DASUGB010000558         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1730931, whole genome shotgun sequence.
ACCESSION   DASUGB010000558 DASUGB010000000
VERSION     DASUGB010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730931"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFV87_10685"
     CDS             <1..626
                     /locus_tag="VFV87_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104269.1"
                     /translation="TSAGLPTSLAPGTPLRQALDDHAAWIAIDALRPDQPGSPDSLDE
                     AAQKLAAALCDDRVLAIYVTTPRRGSPRLMSANAGIREQLAAGQLLGKTGSTEGSEVY
                     LDESSDKDEDAMPWSERRHKLRTLADDARVENAAGYAAVRVRFERGHVREDLWLNVVR
                     SRRTDYRSEEFVGQLTTDSQLWPHLRAGERLRLSFYEPLEIKPLDTP"
     gene            919..1539
                     /locus_tag="VFV87_10690"
     CDS             919..1539
                     /locus_tag="VFV87_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5104270.1"
                     /translation="MTWLFLICAVIGGTVLVCQFVLTLVGLGGDHVDLADNMPDGGDV
                     HVDASHGGDADGHDAHGHHGSSWLFGVISFRTLVAAATFFGLVGLAAESAGQGWGVQL
                     ILATAAGLGAMYGVHWIVRTMARLGEDATERVRAAVGQQGTVYIPIPAARANTGKVQL
                     KLQNRLVEYEAVTSSSQKLATGTKVRVVGVTGKTLEVEPLGVTSAV"
     gene            1684..3315
                     /locus_tag="VFV87_10695"
     CDS             1684..3315
                     /locus_tag="VFV87_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5104271.1"
                     /translation="MTFNALLLGQAGEGMLQFGIISVVIIVFLAIIFFMFIALLVKQY
                     KRCPSNRVLVIFGRTGQGASKTIHGGAAFVVPLIQDYAYLSLEPIQIEVPLRGALSSE
                     NIRVNVPSVFTVAIDTKPEVMQNAAVRLLGLSVPEIRKQAEEMIFGQLRQVIASMGIE
                     EINRDRDKFLEHVQHSLEPELAKIGLQLINVNITDITDESGYIDAIGQKAASLAIQQA
                     RGDVADNEKMGETRVASAERDKSIQVANARKEQSIGTREAQREQAVRVALLEKEQTVG
                     EKQAAFERESQVKVAEQQMRVQVAEADATAIDGENQASARVAASQAELAIKKAEAYER
                     GESRRKIAEAAVLEVQNKSLAKAALAEADKVEAEQRAKLEAPAKAQKAKQIVDAEAAA
                     EQRRIIAQAEADAIYVKLEAEARGQYEILAKKGDGLKQIVTACGGSTQAFQMLMLEHL
                     DNLADASARAISNIKFDKIVVWENGGANGRGSTADFLSGMAKTLPPMMQVMRDIGGVE
                     LPESLVKLGADTSKTEIAGNGSSPNGPGLKEKGKA"
     gene            complement(3426..3866)
                     /locus_tag="VFV87_10700"
     CDS             complement(3426..3866)
                     /locus_tag="VFV87_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104272.1"
                     /translation="MFAAVATIGEVRQLAFHEAVLSDEGLAQLAGLRHVETLTFSGSP
                     ITDASAGVLARFSELTWLDADDTQLTDAAVPHLVKLQKLEVLRIQGTQITDRGFAQLA
                     ALKNLRDLTIGDSAAHPMPVSDRCRDHVQRALPNCRLNGTFRGE"
     gene            complement(3978..4166)
                     /locus_tag="VFV87_10705"
     CDS             complement(3978..4166)
                     /locus_tag="VFV87_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104273.1"
                     /translation="MNRRCVQFGLRTMLGVITASALAGGWLADRMHRRQAAIRTIQAA
                     GGSLTSEFNRRNLIQIFQ"
     gene            complement(4351..>4679)
                     /locus_tag="VFV87_10710"
     CDS             complement(4351..>4679)
                     /locus_tag="VFV87_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104274.1"
                     /translation="RLACVWIQDRAHLETQAATERATKANGVTMQAEQFSLRRMLTAV
                     SIVAVGLALFDYIRPTDRGLADEFLGCIASAAIGCGIGTMYRHPVLGAVVGFCFVAMV
                     LFVTML"
BASE COUNT          911 a         1520 c         1518 g          730 t
ORIGIN      
        1 ctacgtctgc aggcctcccc acatcgctcg cccccggcac tcccctgcgg caggcgctgg
       61 acgaccatgc cgcctggatc gccatcgacg ccctgcgccc cgaccagccc ggctcgccgg
      121 attccctgga cgaggccgcg cagaagctgg ccgctgcgtt gtgcgatgac cgcgtgctgg
      181 cgatttacgt gacgacgccg cgccgcggct cgccgcgctt gatgtcggcg aatgccggga
      241 ttcgcgagca gcttgccgcc gggcaactgc tcggcaagac cggctcgacc gaaggcagcg
      301 aggtctatct cgacgaatcg tcggacaaag acgaggacgc gatgccctgg tccgagcgcc
      361 gccacaagct ccgcacgctg gccgacgacg cccgcgtcga gaatgcagca ggctacgccg
      421 cagtgcgcgt ccgtttcgag cggggccacg tgcgggagga cctgtggctg aacgtcgtcc
      481 gctcgcgacg caccgattac cgcagcgagg aattcgtcgg ccagctcacg acggacagcc
      541 agctctggcc ccacctgcgc gccggcgagc gcctccgcct cagcttctac gagccgctcg
      601 aaatcaagcc gctcgacacg ccgtaacctc tcgctccgcc gcaccgtagg cttctcgctc
      661 cgcgagaagc attgggccgt ccatccgaac agccgtcgcg cttcgcggac cggccgatcg
      721 cggggagttc ccgagcggcg caatgtccgt acgccgaaca cgtccattgc gactcgcgga
      781 gcgaggtcgc ctacgattgg gccgtttgac tttacgaatt gcgtgaccga agatgcgccg
      841 gcgggcaaga ttggaaggga gccagcgaat tactggcaaa tacgtgcttt tcacggtccc
      901 gccttcgggg gcaattccat gacttggctg tttctcatct gcgcggtaat cggcggcacg
      961 gtgctggtct gccagttcgt gctgacgctg gtcggcctgg ggggcgacca cgtcgatttg
     1021 gccgataata tgcccgatgg cggtgacgtt catgtcgatg cttcgcacgg tggcgatgct
     1081 gacggtcatg acgcccacgg ccatcacggc tcgtcgtggc tgttcggggt gatttcgttc
     1141 cgcacgctgg tagcggcggc caccttcttc ggcctggtgg gcctggcggc cgaatcggcc
     1201 ggccaggggt ggggcgtaca actcatactg gccaccgcag ccggcctggg cgcaatgtac
     1261 ggcgtacatt ggatcgtccg cacgatggcc cggctgggag aagatgccac cgagcgggtc
     1321 cgcgccgccg tcggccagca ggggaccgtc tatatcccga ttcccgcggc cagggcgaac
     1381 acggggaagg tgcaactgaa attgcagaat cgtctggtcg aatacgaagc ggtcacgagc
     1441 agcagccaaa agctggcgac ggggaccaag gtccgcgttg tcggcgtcac gggcaaaacg
     1501 ctggaagtcg aaccgctcgg cgtgaccagt gcggtataag gcgcgaagcg gcccgggatc
     1561 gcacgatcgt cgtttaacgc gcagccgaag gcgtagcgcg tacggaaccg ccagcggctc
     1621 gccgttcaac gagcaatggg acgaatctga cccacaagaa atcacaaaca gggggttttc
     1681 aatatgacgt tcaacgcttt gttgctcggg caagccggcg aagggatgct ccaattcggg
     1741 attatcagcg tcgtgatcat cgtctttctg gcgatcatct tcttcatgtt catcgctctg
     1801 ctggtcaagc aatataagcg ctgccccagc aaccgcgtgc tggtcatttt cggccgcacg
     1861 gggcaaggcg caagcaagac gatccacggc ggggccgcgt tcgtcgtgcc gctgatccaa
     1921 gactacgcct acctgagcct ggagccgatc cagatcgaag tgccgctccg cggggcgctg
     1981 tcgagcgaga acatccgcgt caacgtgccg agcgtcttta ccgtggcgat cgacaccaag
     2041 ccggaagtga tgcagaacgc ggccgtccgc ttgctcggtc tttcggtgcc ggaaatccgc
     2101 aagcaggccg aggaaatgat cttcggccag ctcaggcagg tgatcgcgtc gatgggcatc
     2161 gaagagatca accgcgaccg cgacaagttc ctggagcacg tccagcattc gctggagccg
     2221 gagctggcca agatcggcct gcagctgatc aacgtcaaca ttactgacat caccgacgag
     2281 tcgggctata tcgacgccat cggccagaag gccgcctcgc tggcgatcca gcaggcccgc
     2341 ggcgacgtgg ccgacaacga aaagatgggc gagacccgcg tcgcttcggc cgagcgcgac
     2401 aagtcgatcc aggtggccaa cgcccgcaag gagcagtcga tcggcacccg cgaagcccag
     2461 cgcgagcagg ccgtccgcgt ggcccttttg gagaaggagc agacggtcgg cgagaagcag
     2521 gccgcgttcg agcgggaatc gcaggtcaag gtcgccgagc agcaaatgcg cgtgcaggtg
     2581 gccgaagcgg acgccaccgc catcgacggc gaaaaccagg cttccgcccg cgtggccgca
     2641 tcgcaagccg aattggccat caaaaaggcc gaagcctacg agcgcggcga aagccgccgg
     2701 aaaatcgccg aggcggcggt gctcgaagtc cagaataaat cgctggccaa ggcggccctg
     2761 gccgaggccg acaaagtcga agccgagcag cgggccaagc tcgaagcccc cgccaaggcc
     2821 cagaaggcca agcagatcgt cgatgccgaa gcggctgccg agcagcggcg gatcatcgcc
     2881 caggccgaag ccgacgcgat ctacgtcaag ctcgaagcgg aagcccgcgg ccagtacgaa
     2941 atcctggcca agaaggggga cggcctcaag cagatcgtca ccgcgtgcgg cggctcgacg
     3001 caggccttcc aaatgctgat gctggagcat ctcgacaacc tggccgatgc ctcggcccgc
     3061 gcgatctcga acatcaagtt cgacaagatc gtggtctggg aaaacggcgg ggccaatggc
     3121 cgcggcagca cagccgactt cctcagcggc atggccaaaa ccctgccgcc gatgatgcag
     3181 gtgatgcgcg acatcggcgg cgtcgaattg cccgagtcgc tggtcaagct cggcgccgac
     3241 acttcgaaga ccgaaatcgc cggcaatggc tcctcgccca acgggccggg attgaaggaa
     3301 aaaggcaagg cctagcgcaa gttggtccgc cgctccgcga caggatccat ggacggggca
     3361 tgccgcctgc cgggcaatca gatccattgc aatcacaagg ccccgtcagc aatggcgggg
     3421 ccgactcact ccccgcggaa cgtcccgttc agccggcagt tgggcagggc gcgctggacg
     3481 tggtcgcggc agcggtcgct gacgggcatc ggatgcgccg cggagtcgcc gatggtcagg
     3541 tcccgcaggt tcttcagggc ggcgagctgg gcaaatccgc ggtcggtgat ctgagtcccc
     3601 tgaattcgca ggacttcgag cttctgcagc ttgacgaggt gcggcacggc cgcatcggtg
     3661 agctgcgtat cgtcggcgtc cagccaggtc agctcggaaa accgcgccag cacgccggcc
     3721 gacgcgtccg tgatcggaga gccggagaaa gtgagcgttt cgacatgtct caggccggcg
     3781 agctgcgcca gcccttcgtc ggaaagaacg gcctcatgaa aggcgagctg ccgcacctcg
     3841 ccaatcgtcg ccacggcagc gaacaccggg gaatccaggg gccggtcttc cgtgaaggag
     3901 atgccccact gccggtcccc cagcagttgc tcgcgccagc ttttgcggca gacgaccgcc
     3961 tgcccgctgt ccgatggtca ctgaaatatc tggatgaggt ttcgccggtt gaattcggac
     4021 gtcagcgacc cgcccgcggc ctgaatcgtg cgaatggcgg cctgccgccg gtgcatccga
     4081 tcggccagcc acccgccggc cagggcactg gcggtaatca cgcccagcat ggtccgcagg
     4141 ccaaattgaa cgcagcgacg attcatggcg gccctctcgc aaaagggaat gccagccgcc
     4201 cccgcgcacc agccgcaggc ggctgcctac tgtacgccgc ccgcggcaga aagttcgcgc
     4261 ttcgcggccg cgttcgcgcg gtatgcgcgc gaacagttcg ggccgagtgc gcgcgtgatt
     4321 gggcaggcgc tggcggccgt gcgcggtggg ctagagcatc gtcacaaaca agaccatcgc
     4381 cacgaagcaa aagccgacaa ccgcgcccaa aactggatgc cgatacattg tgccaatgcc
     4441 acacccgatg gccgcactgg cgatgcaacc tagaaattcg tcggccaaac ctctgtcggt
     4501 tgggcgaatg taatcgaaca acgccaggcc aacagccact atcgaaaccg cggtcagcat
     4561 gcggcgaagt gagaattgct cagcttgcat cgttacgccg tttgcctttg tcgctctttc
     4621 cgtcgccgct tgcgtttcca aatgcgctct gtcctgaatc caaacacaag ccaagcgat
//
LOCUS       DASUGB010000559         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_481139, whole genome shotgun sequence.
ACCESSION   DASUGB010000559 DASUGB010000000
VERSION     DASUGB010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481139"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1854)
                     /locus_tag="VFV87_10715"
     CDS             complement(91..1854)
                     /locus_tag="VFV87_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5104275.1"
                     /translation="MIRRLALIFALPFFTLHLLTSPSSAEQARPNVILVMTDDQGYGD
                     FSCHGNPVLKTPHLDQLAAESLRLTSFHVTPMCTPTRGQLLTGLDALRNGAMNVSSGR
                     TLLRRGIPTMADLFAAAGYQCGQFGKWHLGDNYPYRPQDRGFHESLWYASSHIGSAAD
                     AWNNDYFDDIYWHNGRREQFEGYTTDVFFREALRWMGEKADANEPFFCYIPTAAAHGP
                     LFVPDRYRQPYKDQPRNVASFFGMLANIDENMGRLESFLKDRKLRDNTIVVFLTDNGG
                     TAGVPVFNAGMRGRKIELYEGGHRAACFIRWPAGKLQPPGEIAELTQVQDLLPTLLDL
                     CGIPKRDDAHFDGISLAGLLRGETDKLPDRKLVIQFSRMNAPVPRRGDACVLWNRWRL
                     VNGDELYDVAADPAQRQNVAEKHPDIVAELRQHYDRWWQAVSPEINHFSRVIIGADAE
                     SPTRLSPCEWADVFLDQMAQVRRGERKNGVWHLEVEMGGKYEFTLRRWPAEADLPIVA
                     ASPEHRGTDGSYPPGVALPIAKARIKIGELDQSAPIGADDKAVTFQTALEKGPVELQT
                     WFYDADGGAICGAYLVEIRRL"
     gene            2013..>2618
                     /locus_tag="VFV87_10720"
     CDS             2013..>2618
                     /locus_tag="VFV87_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEX5104276.1"
                     /translation="MKSRFRATVAGLLALLLAGASVRADEPADRQIQKQFEQTVQPFL
                     RTYCHACHGREEPEAKLDLTAFPTLASVARGHPTWAIVLERLEAGEMPPDDADPQPTA
                     EQRQAVVRWIRAFRKYDAERNAGDPGQVLVRRLSNAEYDYTIRDLTGVDIRPAQQFPV
                     DPANEAGFDNSGESLTMSPALVTKYLGAARHVAAHVALQPQR"
BASE COUNT          458 a          823 c          862 g          475 t
ORIGIN      
        1 gtcggcctgg tcgtatgcgg cctggtcgtg ctaatgctca gcgtgctgcg ttttcgcaag
       61 cagcttgcgt gagacgcgga tcggcgcgga tcacaatctc cgaatttcca ccagatacgc
      121 cccgcagatc gcgccgccgt ccgcgtcgta aaaccaggtc tgcagttcga cggggccttt
      181 ctcgagcgcc gtctggaatg tgacagcctt gtcgtcagca ccaatcggcg ccgactggtc
      241 caattcgccg atcttgatcc gcgccttggc gatcggcagg gcgacgccgg gcgggtaaga
      301 cccgtccgta ccgcggtgct caggcgacgc ggcaacaatc ggcaagtccg cctcagccgg
      361 ccagcggcgg agcgtgaatt cgtacttgcc gcccatctcg acctccaggt gccagacgcc
      421 gttcttccgc tcgccgcggc gcacctgcgc catctggtcg agaaacacgt cggcccattc
      481 gcacggtgaa aggcgggttg gactttcggc atcggcgcca atgatgacgc ggctgaagtg
      541 gtttatttcc ggcgagactg cctgccacca ccggtcgtag tgctgccgca gttccgcgac
      601 gatgtccggg tgcttctcag ccacgttttg ccgctgcgct ggatccgccg ccacgtcgta
      661 cagttcgtcg ccattcacca gccgccagcg attccagagg acgcaggcgt cgccccggcg
      721 cgggaccggg gcattcatcc ggctgaactg gatgacgagc ttgcggtccg ggagcttgtc
      781 cgtttcgccg cgcagcaatc cggccagact gatgccgtcg aagtgcgcat cgtcccgctt
      841 gggaattccg cacaggtcca agagcgtcgg cagcaagtct tgcacctgcg tcagctcggc
      901 gatctcgccc ggtggctgca gcttgccggc gggccagcgg atgaagcagg ccgcgcggtg
      961 gccgccttca tagagttcga tctttcggcc gcgcattccg gcgttgaaaa caggtacccc
     1021 ggcggtccca ccgttgtcgg tcaggaaaac tacgatcgtg ttgtcgcgca gcttgcggtc
     1081 cttcaggaac gactccagcc gacccatgtt ttcgtcaatg ttggcgagca tgccgaagaa
     1141 gctcgccacg ttccgcggct gatccttgta gggctggcgg tatctatcgg gcacaaacag
     1201 cggtccgtgg gccgccgcgg tcgggatgta gcagaagaac ggctcgttcg cgtccgcctt
     1261 ttcgcccatc caccgcaggg cttcgcgaaa gaacacgtcg gtcgtgtagc cctcgaattg
     1321 ctcgcggcgg ccgttgtgcc agtagatgtc gtcgaagtag tcgttgttcc aggcgtcggc
     1381 ggcgctgccg atgtgcgacg atgcatacca cagcgactca tgaaacccgc ggtcctgcgg
     1441 gcgatacgga tagttgtcgc ccaaatgcca tttgccgaac tgcccgcact ggtagccggc
     1501 tgcggcgaag agatcggcca tcgtcggaat cccgcgcctg aggagcgtcc gcccgctgct
     1561 gacgttcatc gccccgttgc ggagggcgtc caggccggtg agcagttgcc cgcgcgtcgg
     1621 cgtacacatc ggcgtaacgt gaaaactcgt cagccgcagg ctctcggccg cgagctgatc
     1681 caggtgcggc gttttgagca cgggattgcc atggcaggaa aagtcgccgt agccctggtc
     1741 gtccgtcatg accaggatca cgttggggcg ggcctgctcg gcggaggaag ggctggttaa
     1801 gagatggagg gtgaaaaatg ggagggcgaa gatcagggca aggcggcgga tcatgggaga
     1861 gtcccttttg acggcgatta aagctaccgt actctaccgg ctgccggggc gagtcgcgag
     1921 caaaacccac gcgaaacggg cacggcctcg gtgtagaatc ggaggtttga acttccaggt
     1981 gttgttgacg tgttccgctt atgccgactt ggatgaaatc cagattccgc gcaaccgtag
     2041 cgggactcct cgcgctgctt ttggctggcg cgagcgtgcg agccgacgaa ccagcagatc
     2101 gccagatcca gaaacaattc gagcagacgg tgcagccctt cctgcggacg tattgccacg
     2161 cctgccacgg ccgggaagag ccggaggcga agctcgacct gacggctttt ccaacgctgg
     2221 cgagcgtcgc gcgcgggcat ccgacgtggg cgatcgttct ggagcggctg gaggcgggcg
     2281 aaatgccgcc ggacgatgcc gacccccagc caacggccga acaaaggcag gccgtagtcc
     2341 gctggattcg ggcctttcgc aagtacgacg ccgagcgaaa cgcgggcgat ccggggcagg
     2401 tgctcgtgcg gcggctgagc aatgcggaat acgactacac gatccgcgat ttgaccggcg
     2461 tcgatattcg cccggcccag cagtttccgg tcgatcccgc aaacgaagcg ggcttcgaca
     2521 actcgggcga atcgctgacg atgtcgccgg cgctcgtcac gaaatacttg ggtgcggcca
     2581 ggcacgtcgc cgcgcacgtc gccctccagc cgcagcgc
//
LOCUS       DASUGB010000560         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1175113, whole genome shotgun sequence.
ACCESSION   DASUGB010000560 DASUGB010000000
VERSION     DASUGB010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175113"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFV87_10725"
     CDS             complement(<1..545)
                     /locus_tag="VFV87_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5104277.1"
                     /translation="MSLLVTGGAGFIGSHLVQRLLAETGSRVVVLDNFNDYYNPQLKR
                     AVAAELKDNERVTVVEGDFRDQDQMAFLLLEHGVWAIVHLGASPGVPASLAHPRQYFE
                     NNVLGTLSLLEAARKYPVERFLFASSSTVYGRGAQAPFVEDAPLGIPASPYGASKRSA
                     EILGLTYHQLFGVPFTSLRLF"
     gene            680..1066
                     /locus_tag="VFV87_10730"
     CDS             680..1066
                     /locus_tag="VFV87_10730"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5104278.1"
                     /translation="MPSRPRALLIVVLAALYLALMFAATHWPARPGGTGMGLDKLVHA
                     GMYAGLAMLILAATGLLRPVTWKVAIAVVLGVALYGAIDEWTQSFVPHRTADFRDWVA
                     DVVGAAAGAAFYMAVSRRSRAANHNR"
     gene            1165..1848
                     /locus_tag="VFV87_10735"
     CDS             1165..1848
                     /locus_tag="VFV87_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104279.1"
                     /translation="MCRTSIVCVVLSIFSSGYVAQQVAAEEAPPTKPMVYVDVCVAEI
                     SLSKLRTLGFDWSHIATFKSNSLHDTVPDPIKFFDALQQYNLAQIRFRPRLATMSGRP
                     ASLHIADTLRLDVVATSQPSEKIQLEYRIDLKLPAPTTPDERRDQKHSVHQLLLDAAT
                     ELVPGETCCVSETRTRRTTENGKTSETATLVLIRADLKPPSDIRTVEGLPMSDVEARY
                     RDVEVRRPR"
     gene            complement(1869..4154)
                     /locus_tag="VFV87_10740"
     CDS             complement(1869..4154)
                     /locus_tag="VFV87_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104280.1"
                     /translation="MAQKRTSTPPASPSASTGSREKAESAPKSPAPLPLGALIGLGLL
                     ALVAAALYYYPAALVSNAALEEVGKLRRTMLGLVMTCVFVPDDVLAMWCGGKVEQFSI
                     VDRMPIALATGAILLGAWLAGALALDGLRITPLLDRLERAVFSLAVGLNLLSLYALAV
                     GLAGGLQQRWLFVMPLVGLVAYGVVKMVRLWRQRDQEPVVTAGDSRRWYWWLLAAAPF
                     ALVIVLGAMLPPSDYDVREYHLQVPKEWHQQGRIAFLPHNIYGNMPLGSELTALWGMA
                     LIGGDDAWWWGALAGKTVMGCYALITAAGLVAFGRRVHSLAAGVFAAVMYLSTPWIVH
                     VSVVGHNEGAVGLYCLTAIYALWLAWKAETPAAGRLALLAGFCAGAAVACKYPPALFL
                     VVPLTIWLAFHPFLRATAWPPRPSSATATALSIAMFLTGVSSGCGLWLAKNAIQTGNP
                     VYPLLCSIFDGRTRTPEKDQQWKRVHSPQPDASGSRFSPGNAAFQLYWLAWGTKWASI
                     ALLPLAAVAWLAREQRGLIALLALWLLFVFAAWWLMTHRLDRFLVPTLPIVASLGGIG
                     AAALPHRAWRIAVIALILWGAITQFPTASWWFQDDNRYFAPLAALRRDDPDLGPLSGK
                     RVEFAHQWLNEHVPPAGRVLLVGDAEPFDLEMPAVYNTCFDDCQFTRLFQGRTREERL
                     AALQDEHITHVFISWFHLARYRSPGNYGYTSDYIQPDLVHDELVRDQRLLRPIPIPLN
                     PDTSQPRMDPAKGELFEVVAE"
     gene            4284..>5001
                     /locus_tag="VFV87_10745"
     CDS             4284..>5001
                     /locus_tag="VFV87_10745"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5104281.1"
                     /translation="MSSSRLCGIFTPNIVPLHADGEINEAELRRYVDWLIDHGVHGLY
                     PNGSTGEFLRFTALERRRIIEIMADQTRGRVPILAGAAEANVKETIKACEHYHTLGCR
                     AVAIVAPFYYKLSPAGVYAYFKEIADHSPIDITLYNIPLFASPIDVPTVQRLAEECPR
                     VVAIKDSSGDLPHMMRMIALVRPLRPEFAFLTGWDAALMPMLLVGCDGGTNATSGVAP
                     EITRKLFDLTMAGKIDEARDL"
BASE COUNT          970 a         1657 c         1526 g          848 t
ORIGIN      
        1 ttgaacaatc gcaggctcgt gaagggcacg ccgaaaagct ggtgataggt cagtcccagg
       61 atctccgccg agcgtttgct cgcgccgtag gggctcgccg ggatgccgag cggcgcatct
      121 tcgacgaacg gcgcctgcgc cccgcggcca tagacggtcg acgacgaagc aaacaggaat
      181 cgctcgaccg gatacttccg cgcggcctcc aacagcgaca gcgtgccgag cacattgttc
      241 tcgaaatact gccgcggatg cgccaggctg gctggcacgc ccggcgaagc gccgaggtgg
      301 acgatcgccc acactccatg ctccagcagc agaaacgcca tctggtcctg atcgcgaaaa
      361 tcgccctcga ccaccgtcac acgctcgtta tccttcagct ccgcagccac cgcccgcttg
      421 agctgcggat tgtagtagtc gttgaagttg tccagcacga caacgcggct gccggtctca
      481 gccagcaggc gctgaaccag gtggctgccg ataaagccgg ctccgccggt gacgaggagg
      541 gacatggggg agggttcagg gttcaggaag gtcaggagtc agattgaatc gatgccaatg
      601 atatacttct gacgcgctca cggcagccat gtgggatcgt cgtaacatct ctgattcctg
      661 acccctgact cctggcctca tgccttcccg cccgcgagcc ttgctgatcg tcgtgctggc
      721 ggcgctgtat ctggccctga tgttcgcggc gacgcattgg ccggcccggc cgggcggaac
      781 cggaatgggc ctcgacaagc tggtccacgc cggcatgtac gccggactcg cgatgctcat
      841 cctggccgcg actggcttgc tgcggccggt aacctggaaa gtcgccatcg ccgtcgtcct
      901 gggcgtggcc ctgtacggcg ccatcgacga atggacgcaa tcgttcgttc cgcaccggac
      961 agcggatttc cgcgactggg tcgctgacgt cgtcggagcg gcagccggag cggctttcta
     1021 tatggcagtc tcgcgccgca gtcgggccgc gaatcacaat cgctagcacg gctcgggctg
     1081 ctaccaaact tgtgaccact gccgcttctt gctataagcc gcgtccctac ccgtccgttt
     1141 gcagttcgcc ttgcggagtg acaaatgtgt cgtacatcaa tagtctgcgt ggtcttgagt
     1201 attttcagct cgggatatgt cgcgcagcag gttgctgctg aggaagcacc gcccacgaag
     1261 ccgatggtct atgtggatgt ttgcgtcgca gagatttcct tatcgaagtt gcgtacgctg
     1321 ggtttcgact ggtctcacat cgcaaccttc aagtcgaatt cgctccacga tacagtaccg
     1381 gacccgatca agttctttga tgcattgcag cagtacaacc tcgcccaaat ccgctttcgg
     1441 ccgcggctgg cgacgatgag tggccggccg gcatctctgc acattgctga cacgctcagg
     1501 cttgacgtag tcgccacatc gcagccctcg gagaagattc agcttgaata tcggattgac
     1561 ctcaagctgc cggctcccac gactcccgat gagcgtcggg atcaaaagca ttccgtccat
     1621 cagcttctgc tcgatgccgc aaccgagctc gttcccggcg agacctgctg cgtcagcgag
     1681 actcgaacac gacgcacgac cgagaacgga aagacttccg agaccgcgac actcgtgttg
     1741 attcgggccg acctcaagcc gccgagcgac atcaggaccg ttgaagggct gcccatgtca
     1801 gacgtagagg cgagataccg cgacgtcgag gtccgtcgac ctcgctaagc acacctgaaa
     1861 ccacccattc attctgcaac aacctcaaac aattcccctt tcgccgggtc cattcgcggc
     1921 tggctggtgt cggggttgag cgggatgggt atggggcgca gcaggcgctg gtcgcggaca
     1981 agctcgtcgt gaacgagatc gggctggatg tagtcgctgg tgtagccgta attgccgggg
     2041 ctgcgatagc gggcgagatg gaaccaggaa atgaagacgt gcgtgatgtg ctcgtcctgc
     2101 agcgccgcca atcgctcttc gcgggtgcgg ccctggaaca gccgcgtgaa ctggcaatcg
     2161 tcgaagcagg tgttatagac ggcgggcatc tccaggtcga acggctcggc gtcgccaacc
     2221 agcaacacgc ggccggctgg cggaacgtgc tcgttgagcc actgatgggc gaattcgacg
     2281 cgtttgccgc tcaaagggcc gagatcggga tcgtcgcgcc gcagtgccgc gagcggggcg
     2341 aaataccggt tgtcatcctg gaaccaccac gaggccgtcg gaaactgcgt gatcgcaccc
     2401 cagagaatca gtgcgatgac tgcaatgcgc caggcccggt gcggtagcgc cgcggcgcca
     2461 atgccgccaa gcgatgcgac gatgggcagc gtcgggacca ggaagcgatc gagccgatgc
     2521 gtcattagcc accacgcggc gaagacaaac agcagccaca gagccaagag cgcgatcagc
     2581 ccgcgctgct cgcgcgccag ccaggccacg gccgccagcg gcaggagcgc gatgctcgcc
     2641 cacttcgtcc cccaggccag ccagtaaagt tgaaacgccg cgttgccggg cgaaaatcgg
     2701 ctgccggagg cgtccggctg cgggctgtgg acgcgcttcc attgctgatc cttttccggt
     2761 gtccgcgtgc ggccatcaaa gatgctgcac agcagcggat agacgggatt gcccgtttgg
     2821 atcgcatttt tggccagcca caacccgcag ccggaactga cgccggtcag aaacatcgcg
     2881 atggagagtg ctgtagctgt ggcactgctt ggtcgcggcg gccaagcagt ggcacgtaaa
     2941 aacgggtgga atgccagcca gatcgtcaat ggcacaacga gaaacagcgc cggcggatac
     3001 ttgcacgcta ctgccgcccc agcgcagaag ccggccagca gcgccagtcg gccggcggct
     3061 ggagtttctg ctttccaggc gagccagagg gcatagatcg cagtcaggca atacaagccg
     3121 accgcccctt cgttgtgtcc aacgaccgac acgtgaacga tccacggcgt tgaaaggtac
     3181 atcacggccg cgaagacgcc cgccgcgagc gaatggacgc gccggccgaa cgcgaccagc
     3241 cccgcagcag taatcagcgc atagcagccc atcacggtct tccccgcgag cgcgccccac
     3301 caccaggcat cgtcgccgcc aatcagcgcc attccccaca gggccgtcag ctccgaaccg
     3361 agcggcatgt tgccgtaaat gttgtgcggc aggaacgcaa ttcgcccctg ctggtgccac
     3421 tccttcggaa cttgcaggtg gtattcgcgg acgtcgtagt cgctcggcgg cagcatcgcg
     3481 ccgaggacga ttacgagcgc gaacggcgca gccgccaaga gccaccagta ccaccgccgc
     3541 gaatcgcccg cggtcacgac gggttcttga tcgcgttgac gccaaagccg aaccattttc
     3601 acaactccgt acgccaccag tccgacaagc ggcattacga acagccaccg ctgttgcagt
     3661 ccgcccgcca gcccaaccgc cagtgcatag agcgacagca agttcagccc caccgccagc
     3721 gagaacaccg cccgctccag ccggtcgagc agcggtgtga ttcgcagccc atcaagcgcg
     3781 agcgctcccg ccagccaagc gccgagcagg atcgcacccg tcgccagcgc gattggcatc
     3841 cgatcgacga tcgaaaactg ctccaccttc ccgccgcacc acatcgccag cacgtcgtcg
     3901 ggcacgaaga cgcacgtcat caccagtccc agcatcgtgc gtcgcaactt gcccacttcc
     3961 tccagcgccg cattgctcac cagggcagcc ggatagtagt agagcgccgc cgcaaccagc
     4021 gcgagcagcc ccaagccaat caacgcgccc agcggcaacg gcgcgggcga tttcggggcg
     4081 gattcggctt tctcgcgcga cccagttgat gcggatgggc tcgccggcgg cgtggaagtg
     4141 cgtttttggg ccacgtgcgt cgtttccaag cgcgagctat gattgacaag cctacctgca
     4201 ccgccggccg gttgcgacga ttctaacgcc tgtgtgcccg cacgttgtcg gccaaatcgg
     4261 tgccacctca ctcagtcccg ctcatgtcca gttcccgcct ctgtggcatc ttcaccccga
     4321 acatcgtgcc gcttcacgcg gacggcgaga tcaacgaagc cgagctgcgg cggtatgtcg
     4381 attggctgat cgatcacggc gtccacggcc tgtatcccaa cggctcgacg ggcgagttct
     4441 tgcggttcac ggccctggaa cggcggcgga tcatcgagat catggccgac cagacccgcg
     4501 gacgtgtgcc gatcctcgcc ggcgccgccg aggccaacgt caaggaaacg atcaaggcct
     4561 gcgaacacta tcacaccctc ggctgccggg cggtggcaat cgtcgcgccg ttttactaca
     4621 agctctcgcc ggcgggcgtc tatgcgtatt tcaaggaaat cgccgatcac tcgccgatcg
     4681 acattacgct ctacaacatc ccgctgttcg ccagtccgat cgatgtgccg accgtgcagc
     4741 ggctggccga ggaatgcccg cgggtcgtgg cgatcaagga cagctcgggc gacctgccgc
     4801 acatgatgcg gatgatcgcc ctcgtccgcc cgctgcggcc cgagttcgcc ttcctcaccg
     4861 gctgggacgc cgccctgatg ccgatgctgc tcgtcggctg cgacggcggc accaacgcga
     4921 ccagcggcgt ggcccccgaa atcacccgca agctcttcga cctgacgatg gcgggcaaaa
     4981 tcgacgaagc ccgcgacctg c
//
LOCUS       DASUGB010000561         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_897312, whole genome shotgun sequence.
ACCESSION   DASUGB010000561 DASUGB010000000
VERSION     DASUGB010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897312"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFV87_10750"
     CDS             <1..505
                     /locus_tag="VFV87_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5104282.1"
                     /translation="DSYFRVEQGLDEIVKSLLQIEEELEWISDLSGAMRLESRLEFVE
                     DRWDDLDSEIRERPRRRRRKLNLADMLKAASGGGGDLSQGSGVNNAVDAYAIMGVEFG
                     SSLADVTTAFRHKAKQLHPDANQGDRSSEPELRRMLEAYQFLKEYLSLSNTEPMKPPD
                     RPYSPAE"
     gene            502..1713
                     /gene="rnd"
                     /locus_tag="VFV87_10755"
     CDS             502..1713
                     /gene="rnd"
                     /locus_tag="VFV87_10755"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX5104283.1"
                     /translation="MNPSVTTRSEPGYITTSASLERLCHQLHASVDLALDTEFVGEDS
                     FVPKLELIQVASPTVSAVIDFAAVQADGPLDAFWELICNPHVTKIVHAGRQDLDLFAL
                     HAGQIPKPFFDTQIAAAMLGFGAQAAYANLVQRIIGTKLAKAHTFTNWSARPLSHDQI
                     AYAAEDVQFLLPIHAHLVKKLSMLGRLDWAYEEFARLESVVGEKGRDPLERYQRIRGW
                     EGLKPKHAAVLRDLAAWRESEAKRRNVPRGRVFRDEVLLQLARHPPKTLNEFKALRGV
                     HSSEVERNGEHIMKTVERALALPPASWPQVPTEKRAEPESIGVVELLQAVLKARAAEA
                     EIAPTLLATSADIQALVEAKEKKNEIALPLLRGWRREVAGNLILQVLNGEVAVRVSRT
                     GTLLFTPESSS"
     gene            1710..2993
                     /locus_tag="VFV87_10760"
     CDS             1710..2993
                     /locus_tag="VFV87_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104284.1"
                     /translation="MIRPEHRSTRRSPPVKRLGIGIIGSGFNARFHLQAFVGVRDADI
                     VGIWSPTRSHAASAAAMARRLSVGQATTYRSIAEMVADPAVDAIWICGPNHTRVENVE
                     EITHAIERGRAELLGIACEKPLARNVAEAKRVASLVTGAGLLHGYLENQVFAPQITRG
                     HALIWGRAAALAGRPYLARATEEHSGPHRPWFWQGRLQGGGVLTDMMCHSTLVVRHLL
                     TKPGEAVSTLRPVSVTSRIASLKWTRPKYARRLVRTMGGNVDYRKQPSEDYASMTIEF
                     ETAEKIRVLGEATTSWSFVGPGLRLSAELLGPEYSMSWNTLDSGLNLFVSRDVTTQPQ
                     EDLLEKQNAESGLMPVVADEAATYGYEAENRHVVQAFLAGRLPSLTFEDGVDVVRILM
                     AAYMSAQEGRTVAFPPRGLDKFVPAVARGNWRAFS"
BASE COUNT          618 a          901 c          995 g          618 t
ORIGIN      
        1 ggacagctat ttccgagtcg aacaaggtct ggacgaaatc gtcaagtcac tgctccagat
       61 cgaggaggaa ttggagtgga tctccgatct gtcaggggcc atgcgcctag agtcccgcct
      121 ggaattcgtg gaagaccgct gggacgacct ggacagcgaa atccgagaac gtccgcggcg
      181 gcggcggcgg aaactcaacc tggctgatat gctgaaggcc gcgagcggag ggggcgggga
      241 tctttcgcaa gggtctggag tgaataatgc cgtggatgcc tatgccatta tgggagtcga
      301 gtttggaagt tcgctggccg acgtgacgac cgcctttcgc cacaaggcca agcagcttca
      361 ccctgacgca aaccaaggcg atcgcagctc cgaaccggaa ctgcgtcgca tgttggaggc
      421 ctaccaattt cttaaagagt acctcagtct gtccaatacc gagccgatga agccgccgga
      481 tcggccatac agtcctgccg aatgaatcca agtgtgacca cacgaagcga gcctgggtat
      541 atcacgacct ccgcgtctct tgaacggctc tgccatcaat tgcatgcgag cgtggacctt
      601 gcgctcgaca cggagttcgt cggggaagat agtttcgttc caaagttgga gcttattcag
      661 gtcgcgtcgc ctaccgtctc cgcagtcatc gactttgcgg ccgtgcaggc cgacgggccc
      721 ctcgatgcgt tctgggaatt gatctgcaat ccgcacgtga ccaagatcgt tcatgccggg
      781 cggcaggatt tggatctgtt cgcgcttcat gcaggacaga taccgaaacc atttttcgat
      841 acgcaaatcg ccgccgcgat gttgggattc ggcgcccaag ctgcgtacgc caatctcgtg
      901 caacgcatca tcggcaccaa gctggccaag gctcatacct tcaccaattg gagcgcacga
      961 ccattgtcgc acgatcagat cgcctatgcg gctgaagacg ttcagtttct gctgccgatt
     1021 cacgcgcatc tggtgaagaa actctcgatg ttgggccggt tggactgggc ctacgaagaa
     1081 ttcgccaggc tggagtcggt ggtgggggaa aagggccggg atcccctcga acgctatcag
     1141 cggatacggg gctgggaagg tctgaaaccc aaacatgccg ccgtgcttcg ggatcttgcc
     1201 gcctggcgcg aaagtgaagc caaacgccgc aatgttccac ggggacgggt gttcagagat
     1261 gaagtcctgt tgcagctggc ccgtcatccg ccgaagacgc tgaatgagtt caaggctctc
     1321 agaggtgtac acagttccga ggtggaacgc aatggcgagc acatcatgaa aacggtcgag
     1381 cgcgcgctgg ccctgccgcc cgcgtcctgg ccgcaagtgc cgacggagaa acgggctgaa
     1441 ccggaatcca ttggcgtcgt cgaactcctt caggccgtcc tcaaggccag ggcagcggaa
     1501 gcggaaatcg ctccgacgct gctcgcgacc agcgccgata ttcaagcact ggtcgaggcc
     1561 aaagagaaaa aaaacgagat cgcgctccca cttttacgcg ggtggcgccg cgaagtggcc
     1621 ggtaatttga tcctgcaagt gttgaacggc gaagtggcgg tgcgtgtgag ccgcacgggc
     1681 acgttgctgt tcacgccgga atcctcttca tgatccgccc ggagcatcgg tcgactcgcc
     1741 ggtcaccgcc agtaaagcga cttggaatcg gaatcatcgg cagcggattc aacgcccggt
     1801 tccatctaca ggcgtttgtc ggtgtgcgcg acgcggacat cgtcggaatt tggagcccca
     1861 cccgttccca tgcggcgagc gccgccgcca tggccagacg actcagcgtc ggacaagcga
     1921 caacctatcg gtccattgcc gagatggtcg ctgatccggc ggttgatgcc atatggatct
     1981 gcggcccgaa tcatacgcgt gtggaaaatg tcgaggaaat cacccatgcg atcgagcggg
     2041 ggcgagccga gcttttggga atcgcctgcg aaaagccttt ggcgcggaac gtggccgaag
     2101 cgaagcgagt cgcctcactg gttaccggcg cgggcctgct ccatgggtat ctggaaaatc
     2161 aggtcttcgc gccgcaaatt acccgcggcc atgcgttgat ctgggggcgc gcggcggctc
     2221 tcgcaggacg gccgtatctg gctcgcgcga ctgaagagca cagcggcccg catcggccgt
     2281 ggttctggca agggcgtctt caaggaggcg gcgtgctcac ggacatgatg tgccattcca
     2341 cgctggttgt gcgccacctg ctcacgaagc cgggagaggc ggtctccacg ctgaggcctg
     2401 tctcggtgac gtcgcgcatc gcaagtttga aatggacgag gccgaagtac gcgcggcgct
     2461 tggttcgcac gatgggcggg aacgttgatt accggaagca accttccgaa gactatgcca
     2521 gcatgaccat cgagttcgag accgccgaga agattcgcgt cctgggggag gctacaacct
     2581 cctggagttt tgtcggtccg gggcttcgcc tctcggccga gttgctgggg ccggagtatt
     2641 ccatgtcgtg gaatacgctc gacagtgggc tcaacctgtt tgtcagccgc gacgtgacga
     2701 ctcaaccgca ggaggacctc cttgagaagc agaatgccga atcgggactg atgcccgtgg
     2761 tggccgatga agcggcgacg tacggctatg aggcggagaa ccgtcatgtc gttcaggcct
     2821 tccttgccgg ccgcctcccg tccttgacgt ttgaggacgg agttgatgtg gtgagaattc
     2881 tcatggctgc ctacatgagc gcccaggagg gccgtaccgt cgccttcccg cctcgaggtc
     2941 ttgacaagtt tgtgcctgca gtggcgcggg gaaactggag agctttctct taggtagcag
     3001 ttttcgcgtt tgtctttccc ggggggttgg tgctgttggc cgccctgggg tttctgcggc
     3061 ccaccggtct gcccgcctgg ctgcagccac caatcatggc gatccccttc ttcgtgctcg
     3121 cattcgggct cg
//
LOCUS       DASUGB010000562         5921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_62945,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000562 DASUGB010000000
VERSION     DASUGB010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62945"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFV87_10765"
     CDS             <1..671
                     /locus_tag="VFV87_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5104285.1"
                     /translation="TLLRRWPQAPLPPPPTDLPSPAEPPDADLLWQTALQQRPDLAAL
                     AWRAEAEEAALALAYKEFFPDVTAVGRYDAAWQEVELRAQVGVALNLPIYREKLRAAV
                     REAQFRLNQRRAEFDQQALEIQYEVIAAARQVEESRQTVELYSQRLVPVAEQNVEAAR
                     ANYENNKLNFLDLAAAQRQLVSILEDQQEAIVALHRRLAELDRVVGVPSLRAGELPPA
                     PPPR"
     gene            966..1235
                     /locus_tag="VFV87_10770"
     CDS             966..1235
                     /locus_tag="VFV87_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104286.1"
                     /translation="MKKLMLTVAAVGLAALMVTEAQAGIFGWLRRGRPDYVQPAPAAT
                     AQVQPGRAFSYEPTAAPTVTYRAYRSGTGGHAYESAINKSLGRVN"
     gene            1405..1947
                     /locus_tag="VFV87_10775"
     CDS             1405..1947
                     /locus_tag="VFV87_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104287.1"
                     /translation="MNAATRMLLLLAIVCGPFLGFATSALAQKGATSKSLGEHTVPFW
                     SSKASSRRLTHARDYARDFHGYIVANPKPEPAVVKEATAEIGRNLEEAKKHFAQMKKD
                     FAGDKDAVAGIEALDKQLAAAFEQHKMLGACCEKADFDAIETMSCCSDLASQLDKLIA
                     AHDELMRRIASKPAAPTAKK"
     gene            1992..2657
                     /locus_tag="VFV87_10780"
     CDS             1992..2657
                     /locus_tag="VFV87_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX5104288.1"
                     /translation="MSDLQTFRRELKARTAAAGKAPSWRSADFDHYMADLQPRRTFFD
                     DTAGTLIAVEIRPRLEALVEQFPGARIMREGQAYRCACWFAQSDRFPVTAKLEFDVEH
                     DKSLQHLYVCSEICLMPVFWNFQPHDKLTLRLDEVDQQRVADWIEQRIFEFLDVYLLH
                     DRGTDDLEEDIVSDPVCGMRLRRSEAREAAEYRGHAYFFCASDCREKFQANPEKFVTL
                     RTM"
     gene            2801..5527
                     /locus_tag="VFV87_10785"
     CDS             2801..5527
                     /locus_tag="VFV87_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX5104289.1"
                     /translation="MEALLERFGESRDVGLSDVEVQAQRARHGWNQLEESPPPPLWQK
                     LLAQFKDLVIWILIVAAVVSGALGEWVDAIAILAIVLLNGVIGFMQEERAQRALAALQ
                     KLSAPVAKVIRSGQLHSLPAKVLVPGDIIELEAGHNVPADGRLVQAFSLNVQEAALTG
                     ESVPVEKDATSILAADAPLGDRRNMVYLGTIAAAGKGTAVITATGMQTELGRIAGMLQ
                     RHEAEPTPLQRRLAGLGRVLVVICLLIVAVIFALEVAREGQFVAAFLTSVSLAVAAVP
                     EGLPAVVTIALALGVQRMVKRNALVRKLPSVETLGSVSVICSDKTGTLTRNEMTVQEI
                     VAGDRHYLVTGVGYAPHGQFLDVSLDAGSNGSTRVDARADGRNPPIDVALHDDLAMAL
                     TIGAWCNNAQLVPQGDAEEAWQVIGDPTEGALVVAALKGHIQARNRSQAVVLEIPFDS
                     ARKAMSVVVRDAGGALALYCKGAPEVILAKCVAERRSGRVEPLSDERRRALLASNAEL
                     AARALRVLALAYRPSPPDRLDQRPESELVFSGLVGMIDPPREEARLAVTKCREAGIRP
                     IMITGDHPATALAVAQKLGMTESGDRAVSGPELERMPDEELAGQVERISVYARASAEH
                     KMRIVTAWKSKGQVVAMTGDGVNDAPAIKAADIGIAMGITGTDVTKEASDMVLTDDNF
                     ASIVSAVEEGRGIYDNIQKVLQFLLSCNAGEILLMFVAGLLGWPAPLLPIQLLWINLV
                     TDGLPALALSLEPPEPDVMRRKPRRRDESILSARLGLAILWQGLLVGLVGLAAFGYSF
                     LNYGDAERARAVTFCVVVYAELLRALAARSQTLTLAQLGPLTNPYLLLAIVVSGLLQV
                     SIAVVPFTQLVFDVPAHTAAEWLIIVALALTPVTLIEVGKPILRRRRATSDL"
     gene            5544..>5921
                     /locus_tag="VFV87_10790"
     CDS             5544..>5921
                     /locus_tag="VFV87_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104290.1"
                     /translation="MLQEKILPLVGPAIGLSLILSLGWSTLAEDAPATAAQNGELQSL
                     LRERRAVLQQAVRHAEEAYRSDQAPLSSAIECQRELAQADLELAGSQEERVSIYKRLV
                     EHARVLEKDAEARFNVGKTTQFDL"
BASE COUNT         1075 a         1894 c         1860 g         1092 t
ORIGIN      
        1 acacgcttct gcggcgctgg ccccaggcgc cgctcccgcc cccgcccacc gatctgccat
       61 ctcccgccga gccacctgac gccgatttgc tttggcagac cgccctgcag cagcggcccg
      121 acctggcggc cctcgcgtgg cgagcggaag cggaggaggc tgccctggcc ctggcgtata
      181 aagagttctt tcccgatgtc accgccgttg gccgctacga tgccgcgtgg caagaagtcg
      241 agctgcgggc ccaggtcggc gtggcgctga acctgccgat ctatcgcgaa aagctccgcg
      301 ccgcggtgcg cgaggcgcag ttccgcctca atcagcggcg agccgagttc gaccagcagg
      361 cgctcgaaat ccaatacgaa gtgatcgccg ccgcccgcca ggtcgaggag agccgccaga
      421 cagtcgaact ctattcgcag cggctggttc ccgtcgccga gcagaacgtg gaagcggcgc
      481 gggcgaacta cgagaataac aagctgaact ttctggacct ggccgcggct cagcggcagc
      541 ttgtgagcat tctggaagat cagcaagaag ccatcgtggc gctccatcgg cgactggcgg
      601 aattggatcg agtcgtgggc gtgccgtcgc ttcgagccgg ggaattgccc cccgctcctc
      661 ctccgcgcta gctcgggcag tctcctgaag ggatgttcga atagcgggag cacaagcgct
      721 tgccgacgcc gccgcgcgcg gtcgcgcgct tgtgtgccaa caacgcgcgc ctgggcgtgg
      781 ccaatgagcg cggccaaccg attatttcta aaccctgtca cgaaaaggag ttacagcaac
      841 gcgatgccgc gggcgacacc cttggcacac cattcgccca atggtgcttt ggaaaccgcg
      901 agacacacgc ccgtgagatc ggcaacgtcc tgtcccgcac aacctttttt gctgggaggt
      961 gtgcaatgaa gaagctcatg ctgacggttg ctgctgtcgg actggcggcc ttgatggtga
     1021 cggaagccca ggcgggaatc ttcggctggc tgcggcgggg tcgccccgac tatgttcagc
     1081 ccgctccggc cgcgactgcg caggtgcagc cgggccgcgc cttctcgtat gagcccactg
     1141 ctgccccgac ggtgacgtat cgggcctatc gcagcggcac aggcggacac gcgtacgaaa
     1201 gtgcgatcaa caagtcgctg ggccgcgtga actagccacc cagcaggcag ttgtccggac
     1261 ggttgccaga gccccggctc attccgagtc ggggctcgcc tttgatccgt cctgcgcgag
     1321 gcggcgccag gagggatcgc gggcagagaa cgtcacgagg cccttggtcc attccacctt
     1381 tcccattttg tcttggagtt ttccatgaac gccgccactc gaatgctttt gcttctcgcc
     1441 attgtttgtg gtcctttcct cggattcgct acctcggccc ttgcccaaaa gggcgctacg
     1501 tcgaagtccc tgggcgaaca taccgtcccc ttctggtctt cgaaagcatc gtctcgccga
     1561 ctgacgcacg cacgggacta cgcccgcgat ttccacggct acattgtggc caatcccaag
     1621 cccgagccgg ctgttgtgaa ggaagccact gccgaaatcg gccgcaatct cgaggaagcg
     1681 aagaagcact tcgcacagat gaagaaggat ttcgctggcg acaaggacgc cgtggccgga
     1741 atcgaggccc tggacaagca gcttgcggcg gcgttcgagc agcacaagat gttgggcgcc
     1801 tgctgtgaga aggccgattt cgatgccatc gaaacgatgt cttgctgcag cgacctcgcc
     1861 tcgcaactcg acaagctcat cgcggcccac gacgagctga tgcgcagaat tgccagcaaa
     1921 ccagcggctc caactgcaaa aaagtaacaa acagcacccg atttgagtct ctcaactgtg
     1981 gcgaggggcg gatgagcgat ttgcaaacct tccgccgaga attaaaggcc cgcacggcag
     2041 cggcgggcaa ggcacctagc tggcgctcgg cagatttcga ccactacatg gctgatctgc
     2101 agcctcgacg tacgttcttc gacgatacgg cggggacgct gattgccgtg gaaatccgcc
     2161 cgcggctgga ggcgcttgtc gagcaatttc ctggcgcgcg catcatgcgc gaagggcaag
     2221 cctatcgttg cgcgtgctgg tttgcccagt ccgaccgctt tccagtcacg gccaaactcg
     2281 aattcgacgt cgagcacgac aaatcgctgc aacacctcta cgtctgcagc gagatctgtc
     2341 tcatgcccgt gttctggaat ttccagccgc acgacaagct caccttgcgc ctggacgaag
     2401 tcgatcagca gcgcgtcgcc gattggattg agcagaggat tttcgagttc ctcgacgtct
     2461 atctgctgca cgaccgcggt accgacgacc tggaagagga tatcgtctcc gatccagtgt
     2521 gcggcatgcg tctccgccgc tcggaggccc gcgaagcggc cgaataccgc ggccacgcct
     2581 atttcttctg cgcctcggat tgccgggaaa agttccaggc gaatcccgag aagtttgtca
     2641 cgctccgcac gatgtgaaca aagggaaata ccgccagttc caacttcctc tgcagatcgg
     2701 taaccgttag taggtttcaa cagcgaacac ttcccttacg atggctgtcg ccgaatcacc
     2761 gccggccaat tcaacaccgg aagtacccca cgcgctcacc gtggaagctc tgctggaacg
     2821 tttcggcgag agtcgggatg tcgggctttc cgacgtggaa gtacaggcgc aacgtgccag
     2881 gcacggctgg aaccagcttg aggaatcgcc tccgccgcct ctctggcaga agctgctcgc
     2941 acaattcaag gacctggtga tctggatcct gattgtggcg gctgtcgtct cgggcgctct
     3001 gggcgagtgg gtcgatgcga tcgctatcct ggccattgtg ctgctcaacg gcgtgatcgg
     3061 cttcatgcaa gaagaacggg ctcagcgagc cctggcggcc ctgcagaagc tttcagcgcc
     3121 cgtcgcaaag gtcattcgca gcggccagtt gcactcgctc cccgcgaagg tgctcgttcc
     3181 gggggacatc atcgagctgg aagccggcca caacgtcccg gcggacgggc ggctggttca
     3241 ggccttcagc ctgaacgtcc aggaggcggc cctcaccggg gaatccgtcc cggtggagaa
     3301 ggacgccacc tccattcttg ccgccgacgc gcctctcggc gaccgccgca acatggtcta
     3361 tctgggcacg atcgccgccg ccggcaaggg cactgccgtc atcacagcca ccgggatgca
     3421 gaccgagcta ggccgtattg ccgggatgct ccagcgccat gaagcggaac ccacgcccct
     3481 gcagcgccgc ctggcggggc tggggcgcgt attggtcgtg atctgcttgc tgatcgtggc
     3541 ggtaattttc gctctggagg tggcgcgcga gggtcagttc gtcgccgcct tcctgacctc
     3601 cgtcagcctg gccgttgccg ccgttcccga gggcctgccc gccgtcgtca cgatcgcgct
     3661 ggctctcggc gtgcagcgga tggtgaagcg caatgccttg gtcagaaaac tgcccagcgt
     3721 ggagacgctc ggatcggtct cggtgatttg ctccgacaag acgggcacgc tgactcgaaa
     3781 cgagatgacc gtccaggaga tcgtggcggg cgaccgccat tacctggtga cgggcgtcgg
     3841 ttatgctccg cacggccaat tcttggatgt ctcgcttgac gcgggatcga acgggagcac
     3901 aagagttgat gcccgagcag atggcagaaa tccccccatc gatgtcgcct tgcacgacga
     3961 cctcgcgatg gcgctgacga tcggcgcctg gtgcaacaat gcccaactcg ttccccaggg
     4021 cgacgctgaa gaagcctggc aggtaatcgg cgatccgacc gaaggagcgc tcgttgtggc
     4081 ggcgctcaag ggccacattc aagccaggaa ccgttcccag gcggtggtgc tggagatccc
     4141 gtttgattcg gctcgcaagg ccatgtccgt cgtagttcgc gatgccggcg gagcgttagc
     4201 gctgtactgt aagggcgccc cggaggtcat attggctaag tgcgtggcag agcgccgcag
     4261 cggccgtgtg gagccgctct cagacgagcg gcgaagggcg ctgctcgctt ctaacgcgga
     4321 attggccgcg cgggccctcc gcgtgctcgc gctcgcctat cgcccctcgc ctcccgaccg
     4381 tctggaccag cgtcccgagt cggaattggt attctcgggc ctcgtgggca tgatcgaccc
     4441 tccgcgggaa gaggcccggc tcgccgtgac caaatgtcgc gaggctggaa ttcggcccat
     4501 catgattacg ggcgaccatc ccgccacggc actcgctgtc gctcaaaagc ttggcatgac
     4561 cgagagcggc gatcgcgccg tctccgggcc ggagttggaa cgaatgcccg acgaggagtt
     4621 ggccggccag gtggaacgca tcagcgtcta tgcccgtgcc tccgccgagc acaagatgcg
     4681 gattgtcacg gcctggaagt ccaaggggca ggtcgtggcg atgaccggcg acggggtgaa
     4741 cgacgcgccc gccatcaagg ccgccgatat cggcatcgcc atggggatca ccggcacgga
     4801 tgtcacgaag gaagcttcgg acatggtgct caccgacgac aatttcgcct caatcgtcag
     4861 cgccgtcgaa gaaggccgcg gcatctacga caacatccag aaagtcttgc agtttctgct
     4921 ctcctgcaac gcgggcgaaa ttctcctgat gttcgtggcc ggtttgctcg gctggccggc
     4981 gcccctactg cccattcagt tgctctggat caatctggtc accgacggcc tgccggccct
     5041 ggcgttgtcg ctggagccgc ccgagccgga cgtgatgcgc cgcaagcccc ggcggcggga
     5101 cgagtcgata ctttccgctc gcctcggcct ggcgattctc tggcagggtt tgctcgtcgg
     5161 actcgtgggc ctggccgcgt tcggctacag cttcctgaac tacggcgacg cggagcgtgc
     5221 gcgggcggtg acgttttgcg tcgtcgtgta tgccgaattg ctgcgggccc tggccgcccg
     5281 cagtcagacg ctgacgctgg cccagcttgg tcccctcacc aatccctatt tgctcctggc
     5341 gatcgtggtg tccggactgc tccaggtcag tatcgccgtc gttcccttca cgcaactggt
     5401 cttcgatgtg ccggcccata cggccgcgga atggttgatc attgtggcgc tggcccttac
     5461 gcccgtaaca ttgattgaag tgggaaagcc aattctgcgc cggaggcgcg ccacatcgga
     5521 cctctaatcc aagagaacct gtcatgctcc aggaaaaaat actacctctt gtcgggccgg
     5581 ccattgggct atcgcttatc ctatccctcg gctggtcaac gctcgcggag gatgcaccgg
     5641 cgaccgctgc ccagaacggc gaactgcagt ctctgctgcg cgagcgccgc gcagtgctcc
     5701 aacaagcggt tcgccacgcg gaggaagcgt accggtccga ccaagccccg ctctcttcgg
     5761 cgatcgagtg ccaacgcgag ctggcgcagg cggaccttga gctggcgggg agccaagagg
     5821 agcgtgtttc catctacaag cggcttgtgg agcatgccag ggtcttagaa aaggacgctg
     5881 aggcccggtt caacgtggga aaaaccacgc agttcgatct t
//
LOCUS       DASUGB010000563         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_203296, whole genome shotgun sequence.
ACCESSION   DASUGB010000563 DASUGB010000000
VERSION     DASUGB010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203296"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(445..>2172)
                     /locus_tag="VFV87_10795"
     CDS             complement(445..>2172)
                     /locus_tag="VFV87_10795"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5104291.1"
                     /translation="KAGIGRLIVINKLDEHNVDFPALIERIRDTFGTNCVLVNVPIGS
                     GADFKGVVSTLGHAGSAAGALVNPEEIHTNLIESIVEADEVLMTKYFEGEEPSQDQLD
                     ALIEHAVAKGTLVPIICCSAKTGVGLTELLDMLIECAPPPTAVHRTGTKDGAEVEIKA
                     DPAGPLVAQVFKTRIDPFVQKLSYIRIFSGTLKKDDTVPVAGSKKGIKLGPLLEVQGG
                     ETKPVDSASAGDIIAIAKMEDLHTGTTFGGIELPKLPFPTPMVGLAVTPKSRNDEAKL
                     STSLHKILEEDSTFKLDRDPQTKELVMTGMSELHLQMIRERLHRRDKLDVETKQPKIP
                     FRETIQAPADGSYRHKKQSGGRGQFGEVHIRMYPFPRGTKPEEYCTKERFPQMKEFHF
                     DEQHNFLWVNSVVGGTIPSNFLPAVEKGFKERIDKGVIAGYTVQDVAVEVHFGKYHDV
                     DSSEAAFKKAGSMAFRNVFQEARPALLEPVVRMEITVPEANVGDVYSDMSGRGGRVQG
                     SDSAGGGFQTVRCEVPLREVTTYARTLSSMTGGQGSFTMEFAHYDVMPGNVQQEVISK
                     ATLHEEEEE"
BASE COUNT          364 a          684 c          685 g          439 t
ORIGIN      
        1 cacgatccgg tcggtcagac gcggcaattg ctctttgagt cgaaagtcgg aagccatgaa
       61 tggtgctccg tgcaggccct gggagtgctt gggtgggcgg gcgacagtgc ccgctgcggc
      121 gctggcgggt cgccgcgctc gcatcgcgcg cggcggtcag aatcacggac gcgggaagca
      181 gcgcgtcacc ccgtacagcg gcaagaagct gagccgaaat gataagagca ggcaggcatc
      241 gaaaatgggc cgcggcagag tccacttaac cggcaaatct tcccgaatcg taggcccgcc
      301 ggcccgccga ttcaagccaa ctgccaatct cttcggccgc ggaatcgacg gaaatgaagg
      361 taaaaccgcc gccgccagtg cgctccgatg ggtcgagcac aatgccagca tcgcggtgaa
      421 acactgtgac tgcgattcgg ccagctactc ctcctcttcc tcgtgcagcg tcgccttgct
      481 gatgacttcc tgctggacgt tgccgggcat tacgtcgtag tgggcgaatt ccatcgtgaa
      541 gctcccctgg ccgccggtca tgctcgacaa ggtgcgggcg tacgttgtga cttcccgcag
      601 gggaacttcg cagcggaccg tctggaatcc gccgccggcc gaatcgcttc cctgcacccg
      661 gccgccgcgg cccgacatgt cgctataaac gtcccccacg ttcgcctcgg gaacggtgat
      721 ctccatccgc acgaccggct ccaggagggc agggcgggct tcctggaaga cattgcgaaa
      781 cgccatcgag ccagccttct tgaacgctgc ttccgaacta tcgacgtcgt ggtatttgcc
      841 gaaatggacc tcgaccgcca cgtcctgcac cgtgtagccg gcgatcaccc ctttatcgat
      901 ccgttccttg aatccttttt ccaccgcggg caggaagttg ctgggaatgg tgccgccgac
      961 caccgagttg acccacagga agttgtgctg ctcgtcgaag tggaactcct tcatctgcgg
     1021 aaagcgttcc ttggtgcagt attcttcggg cttggtgccg cgcgggaacg gatacatgcg
     1081 gatgtgcacc tcgccgaact ggccccgtcc gcccgactgc ttcttgtggc ggtagctgcc
     1141 gtcggccggg gcctggatcg tctcgcggaa ggggattttc ggctgcttgg tttcgacatc
     1201 cagcttgtcg cggcggtgca gccgctcgcg gatcatttgc aggtgcagct cgctcatgcc
     1261 ggtcatcacc agctctttgg tctggggatc gcggtccagc ttgaaggtgg aatcctcctc
     1321 cagaatcttg tgcagcgacg tggagagctt ggcttcgtcg ttgcggctct tgggagtgac
     1381 cgccaggccg accatcggcg tgggaaacgg cagcttcggc agctcaatgc cgccaaaggt
     1441 ggttcccgtg tgcaggtcct ccatcttggc gatggcgatg atgtcgcccg ccgaggccga
     1501 atcgaccggc ttggtctcgc ccccctgcac ctccaagagg ggcccgagct tgatgccctt
     1561 cttcgagccg gcgaccggca cggtgtcgtc ctttttcagc gtgccgctga agatgcggat
     1621 atagctgagc ttttggacga acgggtcgat gcgggtcttg aacacctggg cgacgagcgg
     1681 cccggcggga tcagccttga tttccacctc cgcgccatcc ttcgtccccg tgcgatgcac
     1741 cgcggtcggc ggcggggcgc actcgatgag catgtcgagc agctcggtca ggccgacgcc
     1801 cgttttggct gagcagcaga tgatcggcac cagcgtccct ttggcaaccg catgctcgat
     1861 cagcgcatcg agctggtctt gcgaaggctc ctccccctcg aagtatttgg tcatcagcac
     1921 ttcatcggct tcgacgatcg actcgatcag gttcgtgtgg atttcctcgg gattgacgag
     1981 cgccccggcg gcgctgccgg catggccgag cgtgctgacg actcccttga aatccgcgcc
     2041 gctgccgatg ggcacattga ccagcacgca gttggtgcca aaggtgtcgc gaatccgctc
     2101 gatgagggcg gggaaatcga cgttatgctc gtccagcttg ttgatgacga tcaggcggcc
     2161 gattcccgcc tt
//
LOCUS       DASUGB010000564         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_481449, whole genome shotgun sequence.
ACCESSION   DASUGB010000564 DASUGB010000000
VERSION     DASUGB010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481449"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1764
                     /locus_tag="VFV87_10800"
     CDS             436..1764
                     /locus_tag="VFV87_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5104292.1"
                     /translation="MPVSETSSPPGGGLLASILSEDTFRPAEPRTIEETGLTGTVIEN
                     LLLKYLLLIGSASGRQIADNLCLPFVITEPIYQSLRQRQILVNSASAQFGDFVYTLTD
                     SGRQRAKSAMDTCSYVGAAPVPLDDYIVSVEAQTIRAEAPQKRHLLKAFSDISIDPAI
                     LDMLGPAVNSGAGLFLYGAPGNGKTTLARRITICFGSAIWIPRVITEDGQIIKVFDAA
                     YHEPVDGEKSGLLKTAEHDRRWVKVKRPTVIVGGELTLDALEIRHDPVSNVSEASLQL
                     KSNCGCLLIDDFGRQRISPVDLLNRWIVPLENRIDYLTLATGRKIQVPFDQLIIFSTN
                     LEPTDLADEAFLRRIPYKIEIGDPSREEFLSLFQLCCKSLHCEYNESAIEHLIANHYE
                     PLNRAFRRCHPRDLLSQVRNYCVYTGQPMVMRPELFDKVVPSYFTVLNGQ"
     gene            complement(1819..>2006)
                     /locus_tag="VFV87_10805"
     CDS             complement(1819..>2006)
                     /locus_tag="VFV87_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104293.1"
                     /translation="TIGDNKDVVYETGVYNLTQPETPILVHAGEGRTETYLLTRLEAP
                     EAETPPPPVPNPPGGER"
BASE COUNT          377 a          631 c          648 g          350 t
ORIGIN      
        1 tttgtagttg gtagggtgag ctgtgcccac cgccgcgcgg gcggcggcag ggcgccgcgg
       61 cgagattttc gagatgataa cgcgcaacag cgcaacatcc tcatctgggg ggtgttgcgc
      121 ggcgcaaggc attgatggga ctggacttaa gagaatgttg cgcggggcgt cgggcgtgaa
      181 aaaaacgtgg aaagttgcgc ggtcacctcc ccttggaagg cgatcggaat cgaggcggag
      241 cctcgtggaa gggcgttccc aggcaggagc ccgggaacga gaaagccggg accaggtttg
      301 gtcctggcca agaacaggac ggcggggcga cctcgccgtg acctaggctt gctaagcccg
      361 cggatgcgcg ccggaatcgg tgcgtgcggg gtgtgtcgaa tgtagcgatt gcaaccgttg
      421 ctcggagcgt gcgtcatgcc tgtttctgaa acgagttccc cgccgggcgg cggcttgctg
      481 gcgtcgatcc tctcggaaga caccttccgc cccgcggagc cacggacgat cgaggagacc
      541 ggcctgaccg gcacggtcat cgagaacctg ctgctcaagt acctgctctt gatcggctcg
      601 gccagcgggc ggcagatcgc cgacaacctc tgcctgccgt tcgtgatcac ggagccgatc
      661 taccagtcgc tgcggcagcg gcagattctg gtgaacagcg ccagcgccca gttcggcgac
      721 ttcgtctaca cgctgaccga cagcggccgc cagcgggcca agagcgcgat ggatacgtgc
      781 agctacgtcg gcgcggcgcc cgtgccgctc gacgattaca tcgtctcggt cgaggcccag
      841 acgatccggg ccgaagcgcc gcaaaaacgg cacctgctca aggccttctc cgacatctcg
      901 atcgaccctg cgatcctcga catgctcggc cccgccgtga acagcggcgc gggcctgttc
      961 ctgtacggcg cgccgggcaa cggcaaaacg actctggccc ggcggattac aatctgtttc
     1021 ggcagcgcga tctggattcc gcgcgtgatc accgaagacg gccagatcat caaggtcttt
     1081 gacgccgcct atcacgagcc ggtcgatggc gaaaaatcgg gcctgctgaa aaccgccgag
     1141 cacgaccggc ggtgggtgaa ggtaaagcgg ccgacggtga tcgtcggcgg cgaactgacg
     1201 ctcgacgccc tcgaaatccg ccacgacccg gtcagcaacg tcagcgaggc cagcctgcag
     1261 ctcaaaagca actgcggctg cctcttgatc gacgacttcg gccgccagcg gatttcgccg
     1321 gtcgatttgc tcaaccgctg gatcgtgccg ctggaaaacc gcatcgacta tctgacgctg
     1381 gcgaccggcc gcaagattca ggtcccgttc gaccagctca tcatcttctc gacaaacctc
     1441 gagccgaccg acctggccga cgaagcgttt ctgcggcgga ttccgtacaa gatcgagatc
     1501 ggcgatccgt cgcgcgagga attcctgagc ctgtttcagc tctgctgcaa gagcctgcac
     1561 tgcgagtaca acgaatcggc gatcgagcac ctgatcgcca atcattacga gccgctgaac
     1621 cgggccttcc ggcgctgcca tccgcgcgac ctcttgagcc aggtccgaaa ctactgcgtc
     1681 tataccggcc agccgatggt gatgcggccg gagctgttcg acaaggtggt gccgagctac
     1741 ttcaccgtgc tcaacgggca ataaacgcca aaagccgcga ccagtggtcg cggtttccac
     1801 gaaatcaaga agttcggact accgctcgcc tcccggcggg ttgggaacgg gcggcggcgg
     1861 cgtctctgct tccggcgctt cgagccgggt cagcaggtac gtctccgttc gtccctcgcc
     1921 ggcatggacg aggatcggcg tttcaggctg cgtgaggttg taaacgcccg tttcgtaaac
     1981 cacgtccttg ttgtcgccga tggtga
//
LOCUS       DASUGB010000565         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1175341, whole genome shotgun sequence.
ACCESSION   DASUGB010000565 DASUGB010000000
VERSION     DASUGB010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175341"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1064
                     /locus_tag="VFV87_10810"
     CDS             210..1064
                     /locus_tag="VFV87_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5104294.1"
                     /translation="MQIVITAVGPDNVRLADPIIHYVTGQGANIAEIQMYDHDEEALF
                     AMLLRIHLPGAQLPEIRKALAEIGKLKNLGVRVWSPEERQARPKLAICVTLRPETPLA
                     ILRAIRDGQIKADPAVMIGNRPACRGLAEQFGVPWEMVGDADGRADDERLVSLLDQYN
                     VDYVVLARYMRILPAATCWKYAGGRIINLHHGLLPSFPGPRPYHDAYAGRMLTYGATC
                     HFIVPELDAGNQTINQTTFTVPPGTKLDDIIRLGQEDNEPRCLVEGVRRVVDREVQLH
                     FHRVVARR"
     gene            complement(1131..1520)
                     /locus_tag="VFV87_10815"
     CDS             complement(1131..1520)
                     /locus_tag="VFV87_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104295.1"
                     /translation="MNTDASISQLQAFSFDDGRIEDVRIAGDEAWINFVDWRERRFVI
                     HFTNVAFFQTSGVGDTVGGRVRRNSPEIEQVLEAQEFPGVSEERRKLLRERLVQLEIL
                     GHGGTTDVVFENCEVKEALPSTGSSRT"
     gene            complement(1545..>2025)
                     /locus_tag="VFV87_10820"
     CDS             complement(1545..>2025)
                     /locus_tag="VFV87_10820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5104296.1"
                     /translation="DWETYDRTMRLNVDSVYWLTQRFAKHWIANKVNGRVLMIGSING
                     RLAEPTHSCYDTSKGAIEAMVRTLCVSLAPLGIRVNGLAPGLVRTPLTSIIDRDERFR
                     RWMELHTPSQQVPHSDVCGSGAVYLCSDDAWHVHGQMLLVDGGMSAWQQPDMVDNWR"
BASE COUNT          361 a          711 c          573 g          380 t
ORIGIN      
        1 atcacgtcta ttacaagccg attcaggacg cgatcaaaat gtccgagccg ggcaactttt
       61 gagcggtcgt gacgcacctg cggcctctgc gcttgcaagc cgatgaggta tggttgagcg
      121 aggcggcgct ccaaccctac cgggaggctc acgcctcccg ctcgcctgtc ccgctgtcct
      181 ctgtctcctg tcctctgtcc tcttgcccca tgcagatcgt catcaccgcc gtcggccccg
      241 acaacgtccg cctggcggac ccgatcatcc actacgtgac cggccagggg gcgaacatcg
      301 ccgaaatcca gatgtacgat catgacgaag aggccctctt cgccatgctg ctgcgaatcc
      361 atctgccggg agcgcagctt cccgaaatcc gcaaggccct ggccgagatc ggcaagctca
      421 aaaacctggg tgtccgcgtc tggtcgcccg aagagcggca ggcgcggccg aagctggcga
      481 tctgcgttac cctgcggccc gagacgccgc tggccatcct ccgggccatc cgcgacggcc
      541 agatcaaagc cgaccccgcc gtgatgatcg gcaaccgccc cgcctgccgc ggcctcgccg
      601 agcagttcgg cgtgccgtgg gaaatggtcg gcgacgctga cggccgggcc gacgacgagc
      661 ggctggtgtc gctgctcgac cagtacaacg tcgattacgt ggtcctcgcc cgctacatgc
      721 ggattctgcc cgcggccacc tgctggaaat acgccggcgg gcggatcatc aatttgcatc
      781 acggcctcct tcccagcttt cccggtccgc ggccctatca cgacgcctat gccggccgca
      841 tgctcaccta tggcgcgacc tgccacttca tcgtgccgga gctcgacgcc ggcaatcaga
      901 cgatcaacca gaccaccttc accgtgccgc ccggcacgaa gctcgacgac atcattcgcc
      961 tcggccagga ggacaacgag ccgcgctgcc tcgtcgaagg ggtccgccgc gtcgtcgatc
     1021 gcgaagtgca actgcacttc caccgcgttg tcgcccgcag atagccggga ccaaacctgg
     1081 tcccggagaa gcggcaatcg acttcgattc gcagcagcgt cgttcgttca tcatgtccgg
     1141 ctgctgccag tggatggcag cgcctccttc acttcgcaat tctcaaatac gacgtccgtc
     1201 gttccgccgt ggccgagaat ttccaactgg acgagccgtt cccgaagtag cttgcggcgc
     1261 tcttcgctca cgccaggaaa ctcctgggct tccagaactt gttcgatttc tggagagttc
     1321 cgccgaacgc gcccgccaac ggtatctcca actcccgaag tctggaagaa tgcgacgttc
     1381 gtgaaatgga tgacaaaacg acgctcccgc caatcaacga agttgatcca cgcttcatca
     1441 cccgcgatcc ttacatcctc gattctgccg tcatcgaacg agaaagcttg gagttgggaa
     1501 atactggcat ccgtattcat gtccttcccc cttttgattc ctcttcaccg ccagttgtcc
     1561 accatgtccg gctgctgcca ggcgctcatg ccgccgtcga cgagcagcat ctgcccgtgg
     1621 acgtgccagg cgtcgtcgct gcacagatag acggccccgc tgccgcagac gtcgctgtgc
     1681 ggcacctgct ggctgggcgt gtgcagctcc atccagcggc ggaaacgctc gtcgcgatcg
     1741 atgatgctgg tcagcggcgt ccgtacgagg cccggcgcca ggccgttgac gcgaatcccc
     1801 agcggcgcga gcgacacgca cagcgtcctg accatcgcct cgattgcccc cttgctcgtg
     1861 tcgtagcacg aatgcgtcgg ctccgccaag cggccgttga tcgagccgat catcaggacg
     1921 cggccgttga ctttgtttgc aatccaatgc ttcgcgaacc gctgcgtcag ccaatagacg
     1981 ctgtcgacat tcagccgcat cgtgcggtcg taggtctccc agtcc
//
LOCUS       DASUGB010000566         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_758653, whole genome shotgun sequence.
ACCESSION   DASUGB010000566 DASUGB010000000
VERSION     DASUGB010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758653"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..>2166
                     /gene="glmS"
                     /locus_tag="VFV87_10825"
     CDS             364..>2166
                     /gene="glmS"
                     /locus_tag="VFV87_10825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5104297.1"
                     /translation="MCGIVAYVGPREAQEFLLEGLRRLEYRGYDSAGLATLTPDRRFH
                     VTKAVGRIETLAARLADLPTPGSLGIGHTRWATHGMPTEANAHPHFGGRPGSRSHDGG
                     AETPVLALAHNGVIENYLPLKEKLLAEGYEFASSTDTEVVAHLIASYLKKFAQHPEEF
                     ITDDPYSLPLAAVQKAVSQLRGTYGLVILLRDWPDAIFVARQGSPLVIGVGDGEHFVA
                     SDASPLAGYASKIVYLADHQLAVVTADKLEVAHRETGRIEHAVHDLTIAAADVDLGGF
                     PHYMLKEIFEQPEALENAMRGRLSDEDATAVFGGLNLTPQELQRVQRIVLTACGTSWH
                     AALVGEYLLEEVARIPVEVEYASELRYRNPPVENGTLLFAITQSGETADTLAALRELK
                     RKGHPTLAICNVVGSTIAQEAAGGVYLHAGPEIGVASTKAYTSQCLTLTMLALYFGRM
                     RHLSFEAGQRIIAELRRLPDLLRRALEVNGEVRRLAGKYCHATNVLYLGRQYNFPTAL
                     EGALKLKEISYIHAEGYPAAEMKHGPIALVDADTPSVFIMPQGSVYDKVMSNLEEIKA
                     RGGPVIAITAEGDKDVARLADDVIYVPAVPEYLSP"
BASE COUNT          407 a          695 c          723 g          341 t
ORIGIN      
        1 tcggcgatcg cgtcctggcg cgggagtggc agggccggaa gccctggagc agaggaaggg
       61 cgagtcttcg cggaagcgcg aaacgggtgg atgacggacg gattgatccc tggcagcaaa
      121 cacgtgacgc ccggcggcgt cgagggcatt tatggcaccc cccggttggc aatcgcccgg
      181 cgccaccggt cgcattcaca aaccgttgag tccgttgggt ttaaggcgac ggacttttga
      241 ttgctgcgga aaaaaaggtt gaaaaaataa acgcgcctgg ccgagctcgc gacgtttggc
      301 atgagctgac agcttcggcg gcgtaaactt gcgtttggca acctggcggc aggagcgcgt
      361 gcaatgtgcg gaatcgtggc ttatgtcggt ccgcgggaag cccaggaatt cttgctggag
      421 gggctgcgtc ggctcgagta ccgcggctac gacagcgcgg gtctggccac gctcacgccc
      481 gatcggcggt tccacgtgac caaggcggtg ggccgcatcg agacgctggc cgcccgcctg
      541 gccgacctgc ccacgcccgg cagcctgggg attggccaca cgcggtgggc gacgcacggc
      601 atgccgaccg aggccaacgc ccatccccac tttggcggcc ggccgggcag ccgtagccac
      661 gacggcggcg cggaaacgcc cgtgctcgct ctggcccaca atggcgtgat cgaaaactac
      721 ctgccgctca aggagaagct gctggccgaa ggctacgagt tcgccagttc gaccgacacg
      781 gaggtcgtgg ctcacctgat agccagctat ttgaaaaagt ttgcacaaca tcctgaagaa
      841 ttcattaccg acgatcccta ctcgctgcca ctggccgcgg tgcagaaggc ggtgagccag
      901 ctgcggggca cgtacggcct ggtcatcctg ctgcgcgact ggccggacgc gatcttcgtg
      961 gcccgccagg ggagcccgct ggtgatcggc gtgggagacg gcgagcactt tgtcgccagc
     1021 gacgcctcgc ccctggccgg ttatgcctca aaaatcgtct atctggccga ccatcagctc
     1081 gccgtcgtca cggccgacaa gctggaagtg gcccaccgcg aaaccggccg catcgagcac
     1141 gccgtgcacg acctgacgat cgccgcggcc gacgtcgatc tgggcggctt tccccactac
     1201 atgctcaagg agattttcga gcagcccgag gccttggaaa acgcgatgcg cggccgcctg
     1261 agcgacgagg acgcgaccgc cgtgttcggc ggcctgaacc tcacgccgca ggaattgcag
     1321 cgcgtgcagc gaatcgtgct caccgcctgc ggcaccagct ggcacgcggc cctggtcggc
     1381 gaatacctgc tggaggaagt ggcgcgaatt ccggtcgaag tggaatacgc cagcgagcta
     1441 agataccgca atccgccggt agaaaacggc acgctcctgt ttgcgatcac gcaaagcggc
     1501 gagactgccg acacgctggc cgccctgcgg gaattgaagc gcaaagggca tccgacgctg
     1561 gccatctgca acgtcgtggg gagcacgatc gcccaggaag cggcgggtgg cgtctatctg
     1621 cacgccgggc cggaaatcgg cgtcgcctcg accaaggcct acacctcgca atgcctgacg
     1681 ctcacgatgc tcgccctgta tttcggccgc atgcggcacc tcagcttcga ggcggggcag
     1741 cggatcattg ccgagctgcg ccgcctgccc gacctcttgc ggcgggcgct ggaagtgaac
     1801 ggcgaagtcc gccgtctggc aggcaaatac tgccacgcca ccaacgtgct ctatctcggg
     1861 cggcagtaca actttcccac cgccctggaa ggggccctca agctcaagga gatcagctac
     1921 atccatgccg aaggctatcc ggctgccgag atgaagcacg ggccgatcgc cctggtcgat
     1981 gccgatacgc cgagcgtgtt catcatgccg caggggtcgg tgtacgacaa agtgatgtcg
     2041 aacctggagg agatcaaggc ccgcggcggg ccggtgattg ccatcacggc cgagggggac
     2101 aaggacgtgg cccgcctggc cgacgacgtg atctacgtgc cggccgtgcc cgagtacctt
     2161 tcgccg
//
LOCUS       DASUGB010000567         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_758721, whole genome shotgun sequence.
ACCESSION   DASUGB010000567 DASUGB010000000
VERSION     DASUGB010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758721"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFV87_10830"
     CDS             complement(<1..371)
                     /locus_tag="VFV87_10830"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEX5104298.1"
                     /translation="MPTFKLTLAYDGTEFTGWQRQPDKRTVQEELEAALGRITQVRPK
                     CIASGRTDAGVHALGQVVSFQSETKLDGPTLCKALNAELPEDMLVFKVAEMPDGFHAL
                     RDAVRKRYRYVIEDGRLRDLF"
     gene            492..1124
                     /locus_tag="VFV87_10835"
     CDS             492..1124
                     /locus_tag="VFV87_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5104299.1"
                     /translation="MADVLHRLGDISPERVRLRPFPASAADVVEIERCENRLFELVDG
                     ALVEKVMGHPESRLASAIIIALGKYLEENDIGTVSGEGGMFRLPDNLVRIPDVAFARW
                     ERFPDEESIEASVPEVTPDLAVEVLSEGNTRGEMSRKLREYFAAGVSLVWFVDPKAQS
                     VTVYSSPTRSKVVPLEGTLDGGKVLPGFRLPVAKIFARLKRTRKGKRNGR"
     gene            complement(1121..2251)
                     /gene="asd"
                     /locus_tag="VFV87_10840"
     CDS             complement(1121..2251)
                     /gene="asd"
                     /locus_tag="VFV87_10840"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5104300.1"
                     /translation="MKRVGLIGWRGMVGSVLMDRMQAEGDFAHFEPVFFSTSNVGGSG
                     PKVGGKETGKLLDAKSIEALKKVEVVVTCQGGDYTNEVYPQLRAAGFSGYWIDAASAL
                     RMKDDAVIILDPVNLHVVQDALAKGVKNYIGGNCTVSLMLMGLQGLFQAGLVEWMTSM
                     TYQAASGAGAQYMRELLKQMAVASGSVAEQLADPAAAILDIDRRVAETIRSPAYPTEA
                     FGVPLAGSLIPWIDKDLGNGQSKEEWKGQAETNKILGLAGEKTIPVDGVCVRVGAMRC
                     HSQAVTIKLTKDVPLAEIEGLLASANDWVKVIPNEREPSIRELTPATVTGTLSVPIGR
                     LRKLELERSAKGRYLAAFTCGDQLLWGAAEPLRRMLRILIES"
BASE COUNT          422 a          759 c          733 g          479 t
ORIGIN      
        1 tcgaacaaat cccgcagccg gccgtcttcg atcacgtagc gatagcgctt gcggacggca
       61 tcgcgcagcg catgaaagcc gtccggcatt tcggcgacct taaagaccag catgtcctcg
      121 ggcaactccg cattcagcgc tttgcacagc gtcggcccgt cgagcttcgt ctccgactga
      181 aagctcacca cctgcccgag cgcatgtacg cccgcatcgg tccgcccgct ggcaatgcac
      241 ttcggccgca cctgcgtgat tcgccccaat gccgcctcca actcctcctg cactgttctc
      301 ttgtccggct gccgttgcca cccggtgaat tccgtgccgt cgtaggcaag cgtgagcttg
      361 aaggtgggca tgggatgaac tcagagttgg taccacttac gctatactac ctagagcgaa
      421 agtggatctt ggttccttgg aaggcgaact cgatgttggc agctccgccc gtgacgtctg
      481 gcattgaaac gatggcagat gttcttcatc gattggggga catttcaccc gagcgtgtgc
      541 ggctccgccc gtttccagcg agcgccgcgg acgttgtaga aatcgagcga tgcgagaacc
      601 ggctgtttga gttggtcgat ggggcgcttg tggagaaggt catgggtcat ccggaatctc
      661 gcctcgcttc cgcgatcatc atcgcactcg ggaagtatct cgaggagaac gacatcggaa
      721 ccgtgtctgg cgaagggggt atgttccggc tgcccgacaa ccttgtgcgt attcccgacg
      781 tggcgtttgc gaggtgggaa cgatttccgg acgaagagtc gatcgaggcg agcgtacccg
      841 aagttactcc ggaccttgcg gtcgaagttc tcagcgaagg caatactcgc ggcgaaatgt
      901 cgcgcaagct tcgcgagtac tttgcggcgg gcgtgtcgct cgtctggttc gtcgatccaa
      961 aggcacaatc ggtcacagtt tactcgtcgc ccacgcggtc gaaggtcgtc ccgctggaag
     1021 gaacgctcga cggcggcaaa gtgctgcccg gttttcgcct accggtggcg aaaatcttcg
     1081 cccggctcaa gcggactcgc aagggcaagc gcaacggtcg ttaggactcg atcaaaatcc
     1141 gcagcatccg ccggagcggt tcggcggcgc cccagaggag ttggtcgccg caggtgaagg
     1201 cggcaaggta gcggcccttg gcggagcgtt cgagttcgag cttgcggagg cggccgatgg
     1261 gaacgctcag cgtgccggtg acggtggcgg gagtcagctc gcggatcgag ggctcgcgct
     1321 cgttagggat gaccttgacc cagtcgttgg ccgaggcgag caggccctcg atctcggcga
     1381 gcggcacgtc cttcgtgagc ttgatcgtca cggcctggct gtggcagcgc atcgcgccga
     1441 cgcggacgca aacgccatcg acggggatcg tcttctcgcc cgcgaggccc agaatcttgt
     1501 tcgtctcggc ttgccccttc cactcttcct tgctttggcc attgccgagg tctttgtcga
     1561 tccacgggat caggctgccg gcgagcggaa cgccgaacgc ttcggtcgga taggccgggc
     1621 tgcggatcgt ttcggcgacg cggcggtcga tgtcgagaat cgcggcagcc gggtcggcga
     1681 gttgttcggc cacgctgccg ctggccaccg ccatttgctt cagcagctcg cgcatgtact
     1741 gggcccctgc cccgctggcg gcttgatacg tcatgctggt catccattcg acgaggcccg
     1801 cttgaaacaa accttgcagg cccatcagca tcaggctgac cgtgcagttg ccgccgatgt
     1861 agttcttcac ccctttggcc agtgcgtcct gaacgacgtg caggttgacc ggatcgagaa
     1921 tgatgaccgc gtcgtctttc atccgcaggg cgctggccgc gtcgatccaa tagccgctga
     1981 agccggcggc gcgaagctgc ggatagactt cgttcgtgta gtcgcccccc tggcaggtga
     2041 cgacgacctc gactttcttc agcgcctcga tgctcttggc gtccagcagc ttgcccgtct
     2101 ctttgccgcc gaccttcggc ccgctgccgc cgacgttcga tgtactaaaa aacacgggct
     2161 caaagtgcgc gaagtccccc tccgcctgca tccggtccat caagaccgag ccgaccatcc
     2221 cccgccaacc gatcaggccc acacgcttca cgaatcaacc tcgctctgca acgaacaacg
     2281 gttcacaact ggggttcatc ctagtcccga acgatcatgt cgccaacgac ctggccaatc
     2341 accctggacc atgcgcagcc gcgatgggtt cgccacaatt gccatttgac cgc
//
LOCUS       DASUGB010000568         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_203641, whole genome shotgun sequence.
ACCESSION   DASUGB010000568 DASUGB010000000
VERSION     DASUGB010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203641"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="VFV87_10845"
     CDS             <1..1682
                     /locus_tag="VFV87_10845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5104301.1"
                     /translation="LHLGDDTAIVRLGREMAAAGRVEHEHIVYTSDAGTQDGIDYLVM
                     ELVEGVDLARLVAECGPLPIADACEIVRQAALGLAHIESCGLVHRDIKPSNLMLSLQG
                     IVKILDLGLARLRERPIDEEEATHTGFLLGTADYISPEQIDTPRQADVRSDLYSLGCT
                     LFKLLTGRAPFSDEDHLSATRKIDAHRREPPPSVRSLRPEVAFELDQLLLRLLAKEPK
                     DRPPTAIGVAEKLTGFTSGAELRKLAQQALPALPAEVLPRAEMGHTPPSPSGSTRPLD
                     SAPTPRAATLPGARRRFFRLAAGGGALAVGLLAVAVAASGWLNPPSDRGVPIVTRDGL
                     ADGGQAVAGSGVTTFDLRQPPKELKWEGHVSSPPPFFNENQQMLEVRPDSFQLLAIGK
                     YDGHPGAFEVTIRQSPWHGDAGLFFGYRHEPQPGYKQLAMFQLVRVWHYPLANATGKT
                     VSSEMQVVRERGIIWLNPLHLREEARERKLVTYPKSSDVRLRVEFDELGLKRIALDGV
                     ELPELSAPATNAKYTAADYQGVFGLYAAVSEIDMGPTWFSNFVFIPSEPSP"
     gene            1679..2608
                     /locus_tag="VFV87_10850"
     CDS             1679..2608
                     /locus_tag="VFV87_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104302.1"
                     /translation="MTCTITYPQTHGATNGSNLITACGTWNRPLGSATPSPIRVFGKL
                     YTSGPVDAKPPPDAVPATVNPLNHQDVSNGTWHFNANPLPGAVIGASRTLMIWITADN
                     ASCFDPATPQTFTCCDATSAGCPSAPCLGESTPAPAPAGCADLTVIRTIAPRSYQVGL
                     ADAVVEMIHTAKASSRFQFPNVMLDFSLEHSTKDRAVWAGRGLVADVSLFLAVTRTSC
                     GGLCAELTLQRLVPRGVPAPLIWESLKFDLVKGGTLISMHGDPSSASVLVITPDRGRD
                     RVVQPGAAPERASPERPAPKPPARRTNRRTGRR"
     gene            2686..>3162
                     /locus_tag="VFV87_10855"
     CDS             2686..>3162
                     /locus_tag="VFV87_10855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5104303.1"
                     /translation="MIVPEGVVTSETCLHQCPFVAPLEAAELSVPAGPGVGEKSPDRR
                     RSARKTARSNRPGAQADGPLVSCIMPTGDRREFAILAIRCFLEQDYGQKELVIVDDGA
                     IPLAADELNNPQIRCIRLSARASIGAKRNLACREARGAIIVQWDDDDWHGPRRLSRQ"
BASE COUNT          566 a         1026 c          970 g          600 t
ORIGIN      
        1 gtttgcatct cggcgacgat acggccattg tccgactcgg ccgcgagatg gcggcagccg
       61 gtcgagtcga gcacgagcat atcgtctata cgtccgatgc cggcacccag gacggaattg
      121 actacctggt gatggagtta gtcgaaggag tcgatctggc ccgtctcgtc gccgaatgtg
      181 gcccgctgcc gatcgccgat gcctgcgaga tcgtccgcca ggccgcattg ggccttgcgc
      241 atatcgagtc gtgcgggctg gtccaccgcg acatcaagcc ctcgaatctg atgctctcgt
      301 tgcaaggcat cgtgaagatc ctcgatctcg gcctggcccg cctgcgcgag cggcccatcg
      361 acgaagagga ggcgacgcac acgggttttc tgctggggac agccgactac atctctcccg
      421 aacagatcga cacgcctcgg caggcagacg tgcgatcgga tctctacagc ctgggctgca
      481 cgctattcaa actgctcacc ggccgagccc cattcagtga cgaagaccac ttgtccgcaa
      541 cgcgcaagat cgacgcccac cgtcgcgagc cgccccccag cgtccgctcg ttgcgaccag
      601 aggttgcgtt cgagctggat caattgctct tacgactgtt ggcgaaggag ccaaaggatc
      661 gtcccccaac agcaatcggc gttgccgaga agttgactgg tttcacttct ggcgccgagc
      721 tccgcaagct tgcccaacaa gcgctgcccg ctttgcccgc ggaagtcttg ccgcgggccg
      781 agatgggaca cactcctccg agtcccagcg gcagcacgcg gcccttggat agcgcaccca
      841 cgccccgcgc agcgacgctc cctggggcgc gcagacgttt cttccgcctg gctgccggtg
      901 gcggggcatt ggcggttggt cttctggccg tggccgtggc ggccagcggt tggctgaatc
      961 cgccgtctga tcgcggcgtt cctatcgtga cgcgtgacgg cctggccgat ggcggccagg
     1021 cggttgccgg cagcggagtc accacattcg acctccgcca accgccgaaa gaactgaagt
     1081 gggaagggca tgtttcttca ccgccgccgt ttttcaacga aaaccagcag atgctggaag
     1141 tccggcccga ttctttccag cttctggcca tcggcaaata cgatggccac ccgggcgcgt
     1201 tcgaagtcac catccggcaa tcgccgtggc acggcgacgc agggttgttc ttcggctatc
     1261 ggcacgagcc gcagccgggc tacaaacagc tcgcgatgtt ccagctcgtg cgagtgtggc
     1321 actatccgct tgccaatgcg accggcaaga ctgtgagcag cgagatgcaa gttgtccgcg
     1381 agcggggcat tatctggctg aatccactcc atctgcgcga agaggcgcgc gagcgcaagc
     1441 tcgtgaccta tcccaagtcc agcgacgttc gcctgcgtgt cgaatttgac gaactaggct
     1501 tgaagaggat cgcgctcgat ggcgtcgagc tgccggagct gagtgccccc gcgacgaatg
     1561 ccaagtacac cgcggccgat tatcagggcg ttttcgggct gtacgctgcg gtcagcgaga
     1621 ttgatatggg acccacctgg ttcagcaact ttgtcttcat tccatcggag ccatcacctt
     1681 gacatgcacc atcacctatc ctcaaaccca cggcgctacc aacggatcca acctgattac
     1741 ggcctgcggc acgtggaacc gccctcttgg ctcagcgaca ccctcgccga tccgcgtgtt
     1801 cggcaagctc tataccagtg ggcccgtcga cgccaagccg ccgcccgatg ccgtccccgc
     1861 aacggtcaat ccactgaatc atcaagacgt tagcaacggc acttggcact tcaatgccaa
     1921 tccgctaccc ggcgctgtaa tcggcgctag ccgcactttg atgatctgga tcactgccga
     1981 taatgcctcg tgtttcgatc cagcgacgcc gcagaccttc acctgctgtg acgccacatc
     2041 cgctggctgc ccctcggcgc cgtgcctcgg cgaatcgacg ccggccccag cgccggcggg
     2101 ttgtgccgat ctgaccgtca tccgcacgat cgctccgcgg tcgtatcaag tcgggctggc
     2161 tgatgccgtc gtcgagatga tccatactgc caaggccagc agccgattcc agtttccgaa
     2221 cgtcatgctg gatttcagcc tggagcattc gacgaaggat cgtgcggttt gggcagggcg
     2281 cggactggtc gcagacgtct cgctgttcct ggcggtaacg cgcacttcct gcggcgggct
     2341 ctgtgccgag cttaccttgc agcgtctggt gccgcgtggc gttccagcgc cgctaatatg
     2401 ggaatcgttg aagtttgacc tggtgaaagg gggaacgctg atctccatgc acggcgatcc
     2461 ctcgtcggcc agcgtgctcg ttatcacgcc tgatcgtgga cgcgaccgtg tagttcaacc
     2521 tggggcggcg ccagaacgtg cttccccaga gcggcctgca cccaagccac cggcacgacg
     2581 caccaaccga cggacgggac ggcgatgagc ccggccgtcc ttccatcctg tccgcaccgc
     2641 gggcgcgaag tggcagctga ccgatggctc tgctcgtctc ctcagttgat cgttccggaa
     2701 ggagtcgtca catcggagac ctgtctccat caatgcccct ttgtggcacc acttgaggcg
     2761 gccgagcttt cggtgcccgc cggtccaggg gttggtgaaa agagcccgga taggcggcgc
     2821 tcggcgcgga aaactgcgcg gagcaacagg ccgggggctc aagcggatgg cccgctcgta
     2881 agctgcatta tgcccaccgg cgaccggcgc gagttcgcga ttcttgccat ccgctgcttt
     2941 ctggagcagg actacggtca aaaagaactc gtgatcgttg atgacggggc aatcccgctg
     3001 gctgcggacg agttgaataa tccgcagatt cgctgcattc gcctgagcgc tcgggcgagc
     3061 attggcgcga agcgcaatct tgcgtgccgg gaagcgcgtg gcgcgattat cgtccaatgg
     3121 gatgatgacg actggcacgg accgcgccgc ttgtctcggc ag
//
LOCUS       DASUGB010000569         6685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_758969, whole genome shotgun sequence.
ACCESSION   DASUGB010000569 DASUGB010000000
VERSION     DASUGB010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758969"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..980
                     /locus_tag="VFV87_10860"
     CDS             459..980
                     /locus_tag="VFV87_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104304.1"
                     /translation="MQGNATVAGQQRLAAAANRWLKEYQHLAGWLEAIGVVIALLFGA
                     IQLREARIALESAAEANRIALKSEASDLLVGINAAALDHPEVAGEYAGPRRLHLMRLH
                     YFFRVFELHEQGIFDDDRFSAETRYLRWTIKQPEFRLVWNEFAEQYPTRFRGWVDGMV
                     ISATRESAGDEPD"
     gene            complement(1131..1271)
                     /locus_tag="VFV87_10865"
     CDS             complement(1131..1271)
                     /locus_tag="VFV87_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104305.1"
                     /translation="MREKLDYMHANPVKAGLVERAEDWRWSSARWYTRQQTVGVPISW
                     ID"
     gene            complement(1710..3374)
                     /locus_tag="VFV87_10870"
     CDS             complement(1710..3374)
                     /locus_tag="VFV87_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104306.1"
                     /translation="MNTSYQNRLIRQLRDQQVSVASREKKLEHVNRAEKLLSEIDESR
                     SYPYEYLCFRITEYRPESSAGVALPGKELAHDLRLFVEDVSDAADIRVEDVGEPVHTV
                     EDLSKMFNVSTKTISRWREQGLVSRRFLFDGKRKRVGFLRSSVERFVAQNQERVRRGE
                     RFSQLNEEERRDMVERARRLVRAGGCPAEVARRVAQYMNRSVETVRYTLKQFDQQNPD
                     QAVFPEQVGTLTDEVKQQIYQRSRRGESVDALAKRFCRTRTTVYRVLNEMRARRILEL
                     PLDYMYHASFDKPNIEKDILVPMPQSAVARKVRGPSGLPPYLAALYEVPLLTREQEYH
                     LFRKFNYLKHKATKLRAKLDPMQARTSLMNEIEKLYNEAVQVKNEIVQANLRLVVSIA
                     KRHVSGSEDFFGLVSDGNMSLIRAAEKFDFSRGNKFSTYASWAIMKNYARTIPDEFKR
                     RDRFRTSQDEIFSSKQDQRTDQLGQENAQRLREQQVDRILSRLDERERQIIVSRFGLD
                     HDREPLTLKEVGHEMGVTKERIRQIEARALDKIRQAAEEEHIELPD"
     gene            4011..5453
                     /locus_tag="VFV87_10875"
     CDS             4011..5453
                     /locus_tag="VFV87_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104307.1"
                     /translation="MVFAFAARTLRSLPAPFSVRCTALALVALIAGKSAICGAQDYDL
                     RAAPKAGSHQQVRVTVEIEGKLKLNAAGQEVQHVPLTARADLHYVERMLQSGTSAAET
                     RLARHYYQAEAKIRLRDSELASKLRDERRLILLDANADAATLFSPLGPLTRDELELIE
                     APASSLALTALLPKQPLKLGGQWDLEGLVVSRLLGLEAVSQQNVKCQLESAKEGIAVA
                     SLEGTVSGAVGGVSSDCELKGKLNFDLNKRVVTWLTLAIKENRAIGHAQPGFEVLTTL
                     KMVAAPCAAAVEVNDKALSGLQTTADSGQTLIDFTADKAGFQLLHDRRWRVMIERHDA
                     TILRLVDRGDLISQCNISPLPTFTKGEQLTLEGFQNDVKQALGKDFGQVIEAAQEAGE
                     DGLIIQRVIVAGVAGELPIQWTYYHVTDDKGHRASLVFTIEGNLVERFAQIDRELVSG
                     FRFLPDKLPTPAVAPKEVTARNSESAPAPK"
     gene            complement(5468..6355)
                     /locus_tag="VFV87_10880"
     CDS             complement(5468..6355)
                     /locus_tag="VFV87_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5104308.1"
                     /translation="MQRLPLGPLFYEFSRHNEPRLRIQPGETIVVESEDAFSGQIRTN
                     DDRRDKTKKPYGNPQTGPVWIEGAEPGDSLAISILDIKPTLGKCFTRTSDPKQLAEWL
                     GTECPHGTHVCPIQDGKIHWSDSLTIPLAPMLGCIGTAPDNGVPTTFPAGPHGGNMDI
                     IETAPGNIVYLPVFVPGGYLYLGDAHAAMGHGELSASGLEMPAETTITVNLNKGKKIP
                     GPRIESPTEIMTIVSGCPGERSQAEAFARLILWMEADYGWDRWRAYDLLTHVARISVG
                     YYALGTVAVKVGKEYLRGR"
BASE COUNT         1212 a         2051 c         2072 g         1350 t
ORIGIN      
        1 atatagtgac cctcccaccg cacgcaagat gagtgacagc aaaaccaaac cgcccgcaac
       61 ccctctttgt acacgacggc cccccgcccc gaaaggttcg gcacacgcaa gacttgcggc
      121 gagggcgcgg gcggccgatc caactttctc cacgacctgt cgatggtagt tgccaccgct
      181 catcggaggg gcgtataaga cccagatgtt aatctgggtg ggctgagtgc ttgcttcctc
      241 tcaatggcga ccctcaaagg ccatatttga agccaacctt ttttgggatc tggccggggc
      301 gaatacgtgg acgattgccc cgtccactgc tggtacattc gcttgcagcc aacaaatagc
      361 ctcccgtccg acctaacatc aacggcaacg aaatggcaaa ttctcatccc gcgcaagaac
      421 cggaatcact cgcatccagc gctgacggtt ccccgccggt gcaaggcaac gcaacggtcg
      481 ctggccaaca gcgtctcgcg gcggctgcca atcgctggct caaggagtat cagcatctgg
      541 ccggctggct cgaagcgatc ggcgttgtca ttgcgcttct gtttggggcc attcagcttc
      601 gcgaagccag aatcgccctg gagtctgccg ccgaggcgaa ccgcatcgca ctcaaatcgg
      661 aagcaagtga cttactggtg ggaatcaacg ccgcagcctt ggatcacccg gaggtcgccg
      721 gcgagtacgc cggcccgcgt cggctgcact tgatgcgact gcattatttc tttcgcgtct
      781 ttgaactcca tgagcagggc atctttgacg atgatcggtt ctcggcagaa actcgctact
      841 tgcgttggac gatcaagcag ccggaattcc gtctcgtctg gaatgagttt gccgagcagt
      901 acccgactcg gtttcgaggt tgggtggacg gaatggtaat aagcgctacg agagagtcgg
      961 caggcgacga acccgattag cgacaacccc ggtccattga cgaagattta gccgagaagg
     1021 gtggctttaa acgttcggga cttgtgaccg ccgcttaatc cgcgggtggc ccaggttgct
     1081 cgcaacctgg gcctgcagga ggcaatgtac ctatccgccc gccctgccgg tcagtcgatc
     1141 cacgagattg gcacgcccac ggtctgctgc cgagtgtacc accgtgcgga gctccagcgc
     1201 caatcctcgg cccgctcgac gaggcccgcc ttcaccggat tggcgtgcat gtagtcgagc
     1261 ttctcgcgca aggaggaaag gggtcaggcc tattaaacaa tgaagcctga cccctttttc
     1321 ggcctggaag ccgcacggcc atccagattt tggtcagcga atttgatttg caggggataa
     1381 gtccaataat cgtctttaat tggacttatc ccctttttct ctcttctttt tctctctttt
     1441 tctctccgta gcgctggtcg gcaaatctcc gtcgctggtc gctcatgccc gcgattgtgg
     1501 gcaggtccaa ggtgctggtt caagacgccg cgagtagtag cggggttggt agcgctggac
     1561 cttcgacgga tgcctctttc tggcccaggt tgcgagcaac ctgggccacc cgcggcaggc
     1621 gacgaacccg attagcgaca accccggtcc attgacgaag atttagccga gaagggtggc
     1681 tttaaacgtt cgggacttgt gaccgccgct taatccggca gctcgatgtg ctcttcctcg
     1741 gcggcctggc ggattttgtc cagggcgcgg gcttcgatct ggcggatccg ctccttggtg
     1801 acgcccatct cgtggccgac ttccttgagc gtcagcggct cgcgatcgtg atcgaggccg
     1861 aagcggctga cgatgatctg ccgctcccgc tcgtccagcc gcgaaaggat ccgatcgacc
     1921 tgctgctcgc gcagccgctg ggcattctcc tggccgagct ggtcggtccg ctggtcctgc
     1981 ttggagctga agatctcgtc ctggctggtg cggaagcggt cgcggcgctt gaactcgtcg
     2041 ggaatcgtcc gggcgtagtt cttcatgatc gcccagctcg cgtaggtgct gaacttgttg
     2101 ccccgcgaga agtcgaattt ctccgcggcg cggatcagcg acatatttcc gtcgctcacc
     2161 aggccgaaga agtcctccga gccgctcacg tgacgcttgg cgatcgaaac caccagacgc
     2221 agattcgcct ggacgatctc gttcttcacc tgcacggctt cgttgtagag cttctcgatc
     2281 tcgttcatca ggctcgtgcg ggcctgcatc ggatcgagct tggcccgcag cttcgtcgcc
     2341 ttgtgcttca ggtagttgaa cttgcggaac aggtggtatt cctgctcgcg cgtgaggagc
     2401 ggcacttcgt acagggccgc caggtacggc ggcaggccgc tcggtccgcg gaccttccgc
     2461 gccaccgccg attgcggcat gggcacgagg atgtccttct cgatgttcgg cttgtcgaag
     2521 ctggcgtggt acatgtagtc cagcgggagt tccagaatcc gccgggctcg catctcattc
     2581 agcacgcggt aaaccgtggt ccgggtgcgg cagaagcgct tggcgagcgc atcgaccgat
     2641 tcgccgcgcc gcgaccgctg atagatctgc tgcttgacct cgtcggtcag cgtgccgacc
     2701 tgctcgggga agaccgcctg gtcggggttc tgctggtcga attgcttgag cgtatagcgg
     2761 acggtctcga ccgagcggtt catgtactgg gcgacgcgcc gggcgacttc tgccgggcag
     2821 ccgccggccc gcaccaatcg ccgggcccgc tccaccatgt cccgccgctc ttcctcatta
     2881 agctggctga agcgctcgcc gcggcggacg cgctcttgat tctgcgccac gaagcgctcc
     2941 acgctgctcc gcaggaagcc gacgcgcttc cgcttgccat cgaacaggaa ccggcggctg
     3001 accagaccct gctcgcgcca ccgcgagatg gtcttggtcg agacgttgaa catcttgctg
     3061 aggtcttcga cggtgtggac gggctcgccg acgtcttcca cgcgaatatc ggccgcgtct
     3121 gaaacgtcct cgacgaacag gcgcaggtcg tgcgccagct ccttgccagg caaggccacg
     3181 cccgccgacg attccggacg atattccgtg atgcggaagc agaggtactc gtacggatag
     3241 ctgcggctct cgtcgatttc cgagagcagc ttttcggccc ggttgacgtg ctcgagcttc
     3301 ttttcgcgcg aagcgacgct gacctgctgg tcgcgcagtt ggcgaatcag tcgattttgg
     3361 tagctagtat tcatggttgc tcttctcgaa aattccgccc cgccggtgtt cctgcggctc
     3421 ccaccgcccg gccgagctgt ctcctggtcg cgccgcttcc agccgcacgc cgccagggcg
     3481 gacaaaccgc ccttgacgcc taactcaata cgattcgttt tcggaatagt tctttgttca
     3541 ccacccgtgt gctctgggcc aagtcggaaa atgccaaatc ggcaaaatgc tttacccgtc
     3601 aataattgac agcagtttct gtaccgaacc tcgactgggg cattgccttt gtaactccaa
     3661 gcccatagga aggttgcaac aaccgggtaa cgagttcgcg ctggccggcc cggattggtg
     3721 ctcccctact aaacatctta ccacatttcc gaccgctagc attgctgtaa atcattgaca
     3781 atcaggatgt tatgtcaatg caaactgctc aattcctctg caaatctgga tggcgttgca
     3841 cgattctgga tcgcggcgat cttttcggca ggtgctaaaa agccagttga tgaatgcatc
     3901 ttggatccgg tggacatcgc cagcgtcccg tgtcgatgat gctcgaaatc gtgtcgcttt
     3961 cgatgccgtg cgtctcaaag tgagacgcca ttctcgccac gggacgcacc atggtgttcg
     4021 cttttgctgc acggaccttg cggtcgctcc cagcgccctt ctccgttaga tgcactgccc
     4081 tcgcgctggt tgcactgatc gcaggtaaga gcgcgatttg cggtgcccag gattacgacc
     4141 tgcgtgccgc gccgaaggcg ggctcacacc agcaggtgcg cgtcacggtc gaaatcgaag
     4201 gcaagctgaa gctcaacgcc gccgggcagg aagtgcagca cgtcccgctg acggcaaggg
     4261 ccgacctgca ctatgtcgag cggatgctgc agtccggcac atctgccgcc gaaacgcgat
     4321 tggcccggca ctactaccag gccgaggcca aaatccgcct ccgcgacagc gaactggcga
     4381 gcaaactgcg cgacgagcgg cgtctgattc tcttggacgc caatgcggac gcggccacgc
     4441 tcttttcgcc gctcggaccg ctgacgcggg acgagttgga gctgatcgaa gcacctgcca
     4501 gcagcctcgc acttacggcg ctgctgccca agcaaccgct gaagcttggc ggccagtggg
     4561 atctggaggg actggtcgtt tctcgcctgc tgggcctgga agcggtcagc cagcagaacg
     4621 tgaagtgcca gcttgaatcg gccaaggagg ggatcgccgt cgcctcgctg gaaggcacgg
     4681 tttctggcgc cgtgggcggc gtcagcagcg actgcgagct caagggcaag ctgaatttcg
     4741 acttgaataa gcgagtggtt acctggctga cgctcgccat caaggaaaac cgggcgatcg
     4801 gccacgccca gcccggtttc gaggtgctca ccacacttaa gatggtcgcc gccccatgtg
     4861 ctgccgcggt ggaagtgaat gacaaggccc tgtccggcct gcaaacgacg gccgacagcg
     4921 gccagacgct catcgacttt acggccgaca aagcggggtt tcagcttctt cacgaccgcc
     4981 gctggcgggt gatgatcgag cggcacgacg ccacgatcct gcggctggtt gatcgcggcg
     5041 acctgatcag ccagtgcaac atctcgccgc tgcccacctt tacgaagggt gagcagctca
     5101 cgctcgaagg ctttcagaac gacgtgaaac aggccctggg gaaggacttc ggccaggtca
     5161 tcgaagccgc ccaggaagcg ggtgaagacg gcctgatcat ccagcgcgtg atcgtcgccg
     5221 gcgtcgccgg cgagctgccg atccagtgga cctattacca cgtcaccgac gacaagggcc
     5281 accgcgcctc gctcgtcttc acgatcgagg ggaacctggt cgagcgcttc gcccagatcg
     5341 accgcgagct agtcagcggc ttccgcttcc tgccggacaa gctgcccacg ccggcagttg
     5401 cgccgaagga agtgacggcc cgcaactccg agtcggcgcc tgcgccaaag taagacagcc
     5461 ggccagttta tctgcctcgc aaatactcct tccccacctt caccgccacc gtccccaggg
     5521 cgtagtagcc gacactgatg cgggcgacgt gggtgagcag gtcgtaggcc cgccagcggt
     5581 cccagccgta gtcggcttcc atccagagga tcaggcgggc gaaggcttcg gcttgcgagc
     5641 gctcgccggg gcagccgctg acgatcgtca tgatctcggt gggcgattcg atccgcgggc
     5701 cggggatttt cttgcctttg ttcaggttga cggtgatcgt cgtttcggcc ggcatttcca
     5761 ggccgctggc ggagagctcg ccgtgcccca tcgccgcgtg ggcgtcgccc aggtagaggt
     5821 agccgccggg gacaaagacc ggcagataga cgatgttgcc gggggcggtt tcgatgatgt
     5881 ccatgttgcc gccatgtggg ccggcgggga aggtcgtcgg cacgccgtta tccggggcgg
     5941 tgccaatgca gccgagcatc ggcgcgagcg gaatcgtcag cgagtcgctc cagtggatct
     6001 tgccgtcttg aatggggcag acatgcgtac cgtgcggaca ttcggtgcca agccactcgg
     6061 caagctgctt ggggtcgctg gtgcgggtga agcacttgcc aagcgtcggc ttgatatcga
     6121 ggatcgatat tgccagcgag tcgcccggct cggccccttc gatccagacc ggccccgtct
     6181 gtggattgcc gtagggcttc tttgtcttgt cacggcggtc gtcattggtg cggatctgcc
     6241 cgctgaaggc atcttcgctc tcaaccacga tggtctcgcc cggctggatg cgaagccgcg
     6301 gttcgttgtg ccgggagaac tcgtaaaaga ggggcccgag ggggaggcgc tgcatgggcg
     6361 tgggagactg ggtggggaaa ggaatcgagt gggactctgg aattctgatg ggtggtggag
     6421 cgaaaggcta tacgggcgct gcgcgggcga ctgcgatcgc gagtcggcgc caggaatggg
     6481 ccgggaaaag tttgtgaaat tttgtcctgt acgtttaaat aattctgtcc acttgcgcat
     6541 attaatcgat gagggcggct tcgtaccggc ttcggacaga actcggacgg ttgggggcat
     6601 gggcaaaagt cgggagcccg acgcataaac gcagggcctc ccctcctttc ggcgcaccag
     6661 ccgactgata gcgcaacagc gcaac
//
LOCUS       DASUGB010000570         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1732437, whole genome shotgun sequence.
ACCESSION   DASUGB010000570 DASUGB010000000
VERSION     DASUGB010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732437"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFV87_10885"
     CDS             <1..492
                     /locus_tag="VFV87_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEX5104309.1"
                     /translation="TSFDSFRIRTDDPAFAVSPEGEGESAAATSFATGAPAIASGYDP
                     LDVNRDGYVSPLDALMIINELNSRGEHRLLSQDLQVAGATDTNGDGYVTPLDALLVIN
                     YLNAGLAAGDAPEGESDDSAAADSAFSDTDSWWLSDDVSPDSIAELLSAETDLTPRRP
                     RRR"
     gene            577..1356
                     /locus_tag="VFV87_10890"
     CDS             577..1356
                     /locus_tag="VFV87_10890"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX5104310.1"
                     /translation="MVKQLLIYNSAVPLSSARHANVSVDVNDGYAFSASVNAVPLLAV
                     EFPRASSEYAIVFTADGDDVMPAVVLGVRNEQNLYLSPESHWKADYIPAFIRRYPFIF
                     SSGADGKSLTLCIDESHPGVNWEGRGNALFGPDAKPTPYVEKVLDFLKQFQAQFERTR
                     RFCRRIKDLQLLEPMQATVTTPTGEQLSLGGFLAVSRDKLRALSGEILEKLAKNDELE
                     LLYLHLSSLRNFNEVKERLIGALAARTESRTDAPSQKPAAE"
     gene            1417..2862
                     /locus_tag="VFV87_10895"
     CDS             1417..2862
                     /locus_tag="VFV87_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104311.1"
                     /translation="MRLLTFEEHSSVLAAWWSLASGARTLLYFDAHLDLQQVSTQRLG
                     RLEACTSPQDVAELNKPHHLCPDHGFSYSLEDFLYPSHRLGLIERIIWVAPPHVNTAY
                     THAAFERLQQMDGVQFEELLSFRRVQQSWIEGRLLGVDLTICDYRQLEKMKLPADTLV
                     DIDTDFFIALPDDAPWLRPFELFRELAALSLTPELVTISRSVNSGFTPLRYRFLADHL
                     AALYAGRTADSGHFDRLFDLDALARAGDREAAATGCRSELEWHRTCPATWHLLSLTAS
                     DLREVQAAQHQAAALCPAYHDSILREACAFPARHLPLELPALRALEERFVVGHRSDLE
                     DALTWTALGLLHGHLGRVNAVRECYRQVVRHFGAHPELAFALAKALLQAQRPAEALPL
                     LQVALADDKTAAGAHMLMGTILASAGDASQALAHWQAASALAPAWPQLLDLRSALHAA
                     MGDHREATRLRQRRHEFRQQELGLAQRLQRG"
     gene            complement(2886..3710)
                     /locus_tag="VFV87_10900"
     CDS             complement(2886..3710)
                     /locus_tag="VFV87_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104312.1"
                     /translation="MFTGESSTERSALLTSLVEAEGVPYGREDSFKMSRGSLLTQRFL
                     MGIRTEDVAAARILRWCDALGMPPRLQAEFARHLPEANMVGVGLEDSRDHGVYKTYLE
                     FWDKVRLEVRRTGRINPLLLHLGFKWRAGGDGSDGRIARYVCFPRLSIHDTLSRMAQV
                     YSGATAGTALDQATGIVRRAAVAAPTALFLYVETSEEGNSRTSFDINLYKSELTMGDI
                     DPFLRDLARHYCLPDEQFLPLIDHIGPRLLGHLSGGLDRSGHDVTTLYYETRALEA"
     gene            complement(3828..4373)
                     /locus_tag="VFV87_10905"
                     /pseudo
     CDS             complement(3828..4373)
                     /locus_tag="VFV87_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          825 a         1422 c         1369 g          914 t
ORIGIN      
        1 acttcgttcg actcgttccg catccgcacg gacgacccgg ccttcgccgt ttccccagag
       61 ggcgaaggcg aatcggccgc ggctaccagc ttcgccacgg gcgcgccggc aatcgccagt
      121 ggatatgacc cgctcgacgt caatcgcgac ggctatgtgt cgccgctgga tgccttgatg
      181 attatcaacg agctcaacag ccggggtgag caccgcttac tcagccagga tctgcaagtt
      241 gccggcgcca ccgatacgaa cggagacggc tatgtgacgc cgctggatgc tctgcttgtg
      301 atcaactatc tcaacgccgg cctcgccgcc ggtgacgccc ccgaaggcga gtccgacgac
      361 tccgccgccg cagattcggc gttttccgat acggattcct ggtggctcag cgacgatgta
      421 tctccggaca gcattgctga attgctttca gccgaaacag acctgacgcc gcggcgtcca
      481 cgccgccgct aaacaggact tttgcgcgaa cgagttgtga gccgccaacc ggcgcatcgt
      541 gtgaattccc attctcagcc ccaggagttt cggcccgtgg tcaagcagtt gttgatctac
      601 aattccgcag ttcctctatc ttccgcacgg catgccaatg tttcggttga cgtcaacgac
      661 ggttatgcct ttagcgccag cgtcaatgcc gtgcctctgt tggccgtcga gtttccgcgc
      721 gcgtcgtccg aatatgcgat cgtattcacg gccgatggcg acgatgtcat gcccgcagtc
      781 gtcctcggcg tgagaaacga acagaatttg tacctgtcgc cggagtccca ttggaaggca
      841 gattacattc cggcgtttat ccgccgttac ccgttcatct tttcgtccgg cgcggacggc
      901 aagtcgctca ccctctgcat cgacgagtcg cacccaggcg tcaactggga aggccgtggc
      961 aacgccctct tcgggcccga cgccaaaccc actccctatg tcgagaaagt gctcgacttt
     1021 cttaagcaat tccaggccca gttcgagcgc acgaggcggt tctgtcgccg catcaaggac
     1081 ctccaactgc tcgagcccat gcaggccacg gtaacgacac ccacgggcga gcagctctcg
     1141 ctcggcggct tcctggccgt cagccgggat aaactccgcg cgctaagcgg tgaaatactg
     1201 gagaaacttg ccaagaacga cgagctcgag ttgctctatc tgcacttgtc gtctctgcgc
     1261 aatttcaacg aagtcaaaga gcggctgatc ggcgcgctcg ccgcccggac ggaatcaagg
     1321 accgacgcac ccagccagaa accagccgct gagtagctgc cgcggtcaat cctgggaaag
     1381 cggcaattct ctcacgctgc aggaggtcat gtccgcatgc ggctgctgac cttcgaggag
     1441 cattcgtccg tgcttgcagc gtggtggagc ctcgcgagcg gggcgcgtac cctgctctac
     1501 ttcgacgccc atctcgattt gcaacaggtg tcaacccagc ggctgggccg gcttgaggcg
     1561 tgcacgtcgc cgcaggacgt ggctgagcta aataagccgc atcacttgtg tcccgaccat
     1621 ggattcagtt acagcctcga agactttctg tacccctcgc accgcctggg actgatcgag
     1681 cgcattattt gggtcgcgcc gccgcacgtt aacaccgcct acacccacgc ggccttcgag
     1741 cgcttacagc agatggatgg tgtgcagttc gaagaactgc tcagcttccg gcgagttcaa
     1801 caaagctgga tcgagggccg tttgctgggc gtggatttga cgatttgcga ctaccgccaa
     1861 ctcgaaaaaa tgaagctgcc cgcagatacc ctggtcgaca tcgacaccga ttttttcatc
     1921 gccttgccgg acgatgcgcc ctggctccgg ccatttgagc tgttccgaga attggctgcg
     1981 ctgtcgctta cgccggagtt ggtgacaatt agtcgctcgg tcaatagcgg gttcaccccc
     2041 ctgcgctacc gtttcctggc cgaccatctc gcggcactgt atgcggggcg cacggcagat
     2101 agcggccatt tcgatcgctt gttcgatttg gatgctctgg ctcgggccgg tgaccgcgag
     2161 gccgctgcta caggttgccg atctgagctt gaatggcatc ggacctgtcc ggctacttgg
     2221 catctgctga gcctgacagc cagcgacctg cgcgaggtcc aagccgcgca acaccaggcc
     2281 gctgcgctgt gcccagcgta tcacgacagc atcttgcgcg aggcctgtgc atttcccgcc
     2341 cgccacttgc cgctcgaact gccagctctg cgggcgctcg aagagcgctt tgtcgtcgga
     2401 catcgcagcg atctggagga tgcgctcacg tggactgcac tgggcctgtt gcacggccat
     2461 ctcggcagag tcaatgcggt gcgggagtgt tatcgccagg tcgtccgcca tttcggcgcc
     2521 catcctgaat tggcgttcgc cctggctaag gctctgctgc aggctcagcg gcccgcggaa
     2581 gcgctgccgc tgctgcaggt ggcgctggcc gacgacaaga cggcagccgg cgctcacatg
     2641 ctcatgggca caattctcgc tagcgccgga gatgcttcgc aggcgctggc ccattggcaa
     2701 gccgcatctg cgctggcgcc ggcgtggccc cagttactcg acctgcgcag tgccctgcac
     2761 gcagcgatgg gtgatcatcg agaagcaacg cgacttcgcc agcgacgtca tgaatttcgc
     2821 cagcaggaac tgggactcgc ccagcgcttg cagcgtggct gatcgttgtg attccgcggc
     2881 ggttcttaag cttccaaggc ccgcgtctca tagtacagcg ttgtgacgtc gtggccgctc
     2941 cggtcgaggc cgccagacag gtgccctagc agccgcggcc cgatgtggtc gatcaagggc
     3001 agaaattgct cgtcagggag gcaatagtgc cgtgccagat cacgcaaaaa tgggtcgatg
     3061 tcgcccatcg tgagttcgga tttgtacagg ttgatgtcga atgacgtacg cgagtttcct
     3121 tcttcgctgg tctcgacgta gagaaacaga gccgtgggag ccgccaccgc ggcgcggcgg
     3181 acaatgcccg tcgcctggtc gagtgccgtg cccgctgtgg cgccagagta aacctgagcc
     3241 atgcgtgaca gcgtgtcgtg gatcgacagc cgggggaaac agacgtagcg ggcgatccga
     3301 ccatcgctgc cgtcgccccc agcgcgccac ttgaatccga ggtgcaataa caagggattg
     3361 atccgtccag ttcgtcgaac ttcgagccgg actttgtccc agaattccag gtaggtttta
     3421 tagactccat ggtcgcggct gtcctccagg cctacgccca ccatgttggc ttcgggtagg
     3481 tgccgtgcaa actcggcctg caggcgcggc ggcatgccca gcgcgtcgca ccaccgcaaa
     3541 atgcgagccg ccgcaacgtc ctctgtccgt atccccatca ggaaacgttg cgttagcaag
     3601 ctgccgcgcg acatcttgaa cgaatcttct cggccatagg gaacaccttc tgcttcaacc
     3661 aacgaggtga gcaacgcgga tcgctcagta gaagattcgc cggtaaacat gggtcaaaaa
     3721 ggaatgggca ggccgcattc tgctgaacac gcattgattc gggcatcagt cgatcgaacg
     3781 caccgacagc ggccctgtaa tccttttcgt gtcaggccgg cagaaggtca agtgtggatt
     3841 ggttgaggca tgcggtcttc gaacaggatg gtgaagcggt ttaggagctg ctgccagtgt
     3901 ttgatgggca gggtccattt gcgggaggct tcgcggatag ccatgtacac attcttcaag
     3961 gccgactcct cgctgggatg aatctcgcgg ttcgccgccg ccggcgtttg gcgtcctgga
     4021 gttggtagct ttggcctttg gtctccaggg tggcagcggt cggccagcca gtcgagggcg
     4081 gccccggtaa ggcactcgct ccccaggacc tcggtccagt tctcgaacgg caggctagtc
     4141 gtgacgatca ggctgttccg ctcgtaggcg gaggctacca catcgaacag gagctcggct
     4201 gcggccttgc cggccggggc gtagctcagt acgtcgagga tctagaggtc cagcttggca
     4261 agttgctgcc gcagccgcaa gagttgccgc tcgtccttgg cctccaggag cagcgcgatc
     4321 agctcggtga cgcggaaaaa gcggaccttg cggccttggg cgcaggccgc catccctagc
     4381 gccgtggcca ggtgcgactt gccggtccgg cgatattctg cctcccagaa tgctttggcg
     4441 ctatccggca caaccgtctc ggcaagccac tgcaaattct ccgaccgctt cggcgcgctg
     4501 ctttctgcga tgccgctcgc gccacttatt
//
LOCUS       DASUGB010000571         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1175821, whole genome shotgun sequence.
ACCESSION   DASUGB010000571 DASUGB010000000
VERSION     DASUGB010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175821"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VFV87_10910"
     CDS             <1..1334
                     /locus_tag="VFV87_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5104313.1"
                     /translation="QVRSRLKDLDGLRVSVRNLTSLRQGAPVDIDFSITGPDIAQLAE
                     FSDKVRLAAAEIPGIVDVDTTLRLDKPELLTHIERKRAAALGVSVEEIGQTLRVAVGG
                     DDRVSRYRDDTVDDAYDVELRLVNLDRGDRASISQLYVRANPALAARPLTGGGGAEGA
                     AAEGPVLTRIDNVVTFEFGQAAARIDRLDRQRMVAVRANVAAGYALADRIAALQKAAD
                     DIGVPPGFETRVLGRGRELERTLADFRWTFLLSFVFMYIILAAQYEHLIHPFTILLSL
                     PLAVPFGLISLYWGGETLNLYSALGILVLFGVVKKASILQVDHTNALRAQGLPRLEAI
                     LQANRDRLRPILMTTISFVAGLLPLLIATGPGAEERRSIAVLAVGGQTLSLLLTLLAI
                     PVIYSYLDDIGLLWHWLFSPHPAAAAAPEDPVAALEEVAPELASEPAAAGS"
     gene            complement(1389..2768)
                     /locus_tag="VFV87_10915"
     CDS             complement(1389..2768)
                     /locus_tag="VFV87_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104314.1"
                     /translation="MKTCANTQRVTGRRAFLQRTSAGFGWLALTALHAQWSRAAEPKA
                     YQSPLAPKQPHFGARTKRVIFLFMAGGPSHVDTFDWKPELAKAGKGGARGRYLAPVFK
                     FQPSGDSGLMISEIFPHLAEHADDLCLLNGMHTAVAGHQQATVTLHTGSENFVRPSLG
                     AWCVYGLGSMADDLPGFITINPIGDLGGAQNYGSAFLPAVYQGTRLGSGQRGVPNIDN
                     RHLAAGDQRKQLDFVERANRRLLAEDPDDPNLQGLLESYELAFKMQTSVPSTLDLTRE
                     PASIQRQYGLDDPATQAFATQCLMARRLAEKGVRFIQLTSQGWDQHNNLRQAHGRNAT
                     SIDKPIAGLIADLKQRDMLRDTLIVWGGEFGRTPRDDKGDGNGRGHANKGYSMWMCGG
                     GVKGGIRYGATDALGDVAVEGRMGIRDLHATILHLLGLDHEALTFRYAGRDFRLTDTS
                     GEVAHGILA"
     gene            complement(2782..>4465)
                     /locus_tag="VFV87_10920"
     CDS             complement(2782..>4465)
                     /locus_tag="VFV87_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5104315.1"
                     /translation="KAFEALVDRLLASPRFGERWGRHWLDIVRYGDSTGRAWNAPLTY
                     AWRYRDYVIDSLNADKPLDQFVTEQLAGDLLPAKTVAQKREQQIATGMLALGALDLQS
                     LSHEQFVLDGVDDQIDVTTRSLLGLSIACARCHDHKYDPVTMHDYYALAGIFYSTEPL
                     PGTAHQREFGREGYVHPSHLRLLPISLQPKAALPERVGGIHSMSDYQEVWRQGERKIR
                     FTTDPNLAMGVREGDIRDCEIRRQGEPSDRLEAPPRGDIRIPGLPRMDAIDADSSGRL
                     ELARWIMADDNPLTARVLANRIWHKLFGTGLVRTVDGFGFNSEPPTHPELLDHLATQL
                     REDGWSLKRLIRSIVLSHTYQLSSAGQPAALAKDPQNDLHWRMNRRRLELEPLRDSLL
                     AVAGRLTTERPAGIQVAGIGGKSRLSQVASLLPLDSPYRTVYLPVIRSGLPEEYGTFD
                     FPDPCLLQGQREVTTVAPQALFFLNGDFVVRSAREAAGELLESGPTSDDQRVTWAYQR
                     VLQRPPTADETAEAIKLIHGLEPDAGERDPVRYRWSALIQALLCSAEFRYLR"
BASE COUNT          700 a         1471 c         1525 g          769 t
ORIGIN      
        1 cccaggtccg cagccggttg aaggacctcg acggcttgcg ggtgagcgtg cggaacctga
       61 cttcgctgcg gcagggagcc ccggtcgata tcgacttctc gatcaccggt ccggatatcg
      121 cccagctcgc cgaattcagc gacaaagtcc gcctggcggc ggcagaaatt cccggcatcg
      181 tggatgtcga tacgacgctc cgcctcgaca agccggagct cttgacgcat atcgaacgca
      241 agcgggcggc ggcgctgggc gtgagcgtcg aggagatcgg ccagacgttg cgggtggccg
      301 tcggcggcga cgatcgcgta tcgcgctatc gcgatgatac ggtcgatgac gcgtacgacg
      361 ttgagctgcg cctcgtgaac ctcgaccgcg gcgaccgcgc gtcgatttcg cagctttatg
      421 tgcgggccaa tccggcgctg gcggccaggc cgctgaccgg cggcggcgga gcggaagggg
      481 ccgcggcgga ggggcccgtg ctgacgcgga tcgacaatgt cgtcacattc gagttcggcc
      541 aggcggcggc ccgcatcgac cggctggacc gccagcgaat ggtggccgtg cgggcgaacg
      601 ttgccgccgg ctacgccttg gccgaccgga tcgcggccct gcagaaggcg gcggacgaca
      661 tcggcgttcc gccgggcttc gagacgcgag tcctcggccg cggccgcgag ctggagcgga
      721 cgctagccga tttccgctgg accttcctgc tctcgttcgt gttcatgtac atcattctgg
      781 ccgctcagta cgagcacctg atccacccgt tcacgatcct cctctcgctg ccgctggcgg
      841 tgccgttcgg cctgatcagc ctgtattggg gcggcgaaac gctgaacctg tattcagccc
      901 tgggcatcct cgtgctgttt ggtgtcgtca agaaggcatc gatcttgcag gtcgatcata
      961 cgaacgccct gcgcgcccag ggcctgccgc ggctggaagc gatcctgcag gccaaccgcg
     1021 accgcctgcg accaatcctg atgacgacga tctcgttcgt cgccggcctc ttgccgctct
     1081 tgatcgccac cggccccggc gcggaagaac gccgctcgat tgccgtcctc gccgtcggcg
     1141 gtcagaccct ctcgctcctc ctcacgctgc tggcaattcc ggtcatctat tcctacctcg
     1201 acgacatcgg cctcctctgg cactggctat tctctccaca cccagcagcc gctgccgcac
     1261 cggaagatcc cgtcgccgcg ctcgaagaag tcgcgcctga actcgccagc gaacccgccg
     1321 ctgccggctc gtagggtggg ctgtgcccac cctatgtcgt tgccaatgga tggtgggcca
     1381 agcccatcct acgccagaat tccgtgagca acctctccgc tggtgtcggt gaggcggaag
     1441 tcgcggccgg cgtagcggaa ggtgagggct tcgtgatcga ggccgaggag gtgcaggatc
     1501 gtggcgtgca ggtcgcggat gcccattcgg ccttccaccg cgacatcgcc cagcgcgtcg
     1561 gtcgcgccgt agcgtatgcc ccccttcacg ccgccgccgc acatccacat cgaatagccc
     1621 ttgttggcgt gcccgcggcc gttgccgtcc cccttgtcgt cgcgcggcgt gcggccgaac
     1681 tcgccgcccc aaacgatcag cgtgtcccgc agcatgtccc gctgcttcag gtcggcgatc
     1741 aggccggcga tcggcttgtc gattgaggtg gcgttgcggc cgtgggcctg gcggaggttg
     1801 ttgtgctggt cccagccctg gctggtgagc tggatgaacc gcaccccttt ctcggccagc
     1861 cggcgagcca tcaggcactg cgtggcgaag gcttgcgtcg ccggatcatc gaggccgtac
     1921 tgtcgctgga tcgacgccgg ctcccgcgtc aggtcgagcg tgctgggcac ggaggtctgc
     1981 atcttgaacg ccagctcgta cgattcgagc agcccctgca ggttcggatc gtccgggtcc
     2041 tcggccagca ggcggcggtt ggcccgctcg acgaagtcga gctgcttgcg ctggtcgccg
     2101 gcggccagat gccggttgtc gatgttgggc acgccgcgct gcccgctgcc cagacgcgtg
     2161 ccttgataga cagccgggag gaaggcggag ccgtagtttt gggccccgcc gaggtcgccg
     2221 atcggattga ttgtgataaa gcccggcagg tcgtcggcca tcgatccgag gccatagacg
     2281 caccacgcgc cgagcgacgg gcggacgaag ttctcgctgc cggtgtgcag cgtcacggtc
     2341 gcctgctggt ggccggcgac ggcggtgtgc atgccgttca agagacacag gtcgtcggca
     2401 tgctcggcca ggtgcggaaa aatctccgaa atcatcagcc cgctgtcgcc gctgggctgg
     2461 aacttgaaga ccggcgcaag ataccggccg cgggcgccgc ccttgccggc tttggccagc
     2521 tcgggcttcc agtcgaacgt atcgacgtgg ctggggccgc ccgccatgaa caggaagatc
     2581 acccgcttgg tccgcgcacc aaagtgcggc tgcttcggcg cgagggggct ttggtatgcc
     2641 ttcggctccg cggcccgcga ccactgggca tggagcgccg tcaaagccag ccagccgaag
     2701 ccggcgctgg tgcgctggag gaaggcccgg cggccggtaa cgcgctgggt attggcacag
     2761 gtcttcatgg gcagacgctc cttagcggag gtagcgaaat tcggcggaac agaggagcgc
     2821 ctggatcagc gccgaccagc gatagcggac cggatcgcgc tcgccggcgt cgggctccag
     2881 gccgtgaatc agcttgatgg cctcggccgt ttcgtccgcg gtcggcggtc gctgcaggac
     2941 gcgctgatag gcccaggtca cgcgctggtc gtcgctggtc gggccgcttt cgagcaattc
     3001 gccggccgct tcgcgggccg agcgaacgac gaagtcgccg ttcaaaaaga acagcgcctg
     3061 cggggcgacg gtcgtcactt cgcgctgccc ctgcagcagg cacggatcgg gaaaatcgaa
     3121 cgtgccgtac tcctcgggca agccggagcg aattaccggc agatagacgg tgcgatacgg
     3181 cgaatcgagc ggtaaaagac tggcgacctg cgagaggcgc gatttgccgc cgatgccggc
     3241 gacctggatt cccgcgggac gctccgtcgt cagccgcccg gccacggcca gcagcgagtc
     3301 gcggagcggc tcgagttcca gccggcggcg gttcatccgc cagtgcaggt cattctgcgg
     3361 gtctttcgcc agggctgccg gctggccggc gctactgagc tgatacgtgt ggctgaggac
     3421 gatcgagcgg attaggcgct tcagcgacca gccatcctcg cgaagctgcg tcgccaggtg
     3481 gtcgagcagc tcaggatgcg tcggcggctc cgagttgaag ccgaaaccat cgaccgtccg
     3541 caccagtccc gtgccgaaga gcttgtgcca gatgcgattg gccagcacgc gagccgtcag
     3601 cggattgtca tcggccatga tccagcgggc gagctccagc cggccgctgg aatcggcatc
     3661 gatcgcatcc atgcgcggca gtccgggaat gcggatgtcg ccgcgcggcg gcgcttcgag
     3721 gcggtcgctc ggctcaccct ggcggcggat ttcgcagtcg cgaatgtctc cctcacggac
     3781 gcccatcgcc aggttcggat cggtcgtgaa gcgaatcttc cgctccccct gccgccagac
     3841 ctcctgatag tcgctcatgg agtggatgcc gccgacgcgc tcgggcagcg ccgctttcgg
     3901 ctgcagcgaa atcggcagca accgcaagtg gctcgggtgt acgtagcctt cgcggccaaa
     3961 ttcgcgctga tgggccgtgc ccggcagcgg ctcggtgctg taaaagatgc cggccagcgc
     4021 gtagtagtcg tgcatcgtga cggggtcgta cttgtggtcg tggcagcggg cgcaggcgat
     4081 tgagaggccg agcagggacc gcgtcgtcac atcgatctgg tcatcgactc cgtcgaggac
     4141 gaactgctcg tggctgagcg attgcaggtc aagcgcacct aacgcgagca tgcccgtcgc
     4201 aatttgctgc tcgcgctttt gagccacggt cttcgccggc aagaggtcgc cggcaagctg
     4261 ctcggtgacg aactggtcca gcggcttgtc ggcgttgagc gaatcgatca catagtcgcg
     4321 ataccgccag gcatacgtca gcggagcgtt ccaggcccgc ccggtgctgt cgccatagcg
     4381 gacgatatcg agccaatgcc gcccccaccg ctcgccgaaa cggggcgaag cgagcagccg
     4441 atcgacgagc gcctcaaatg ccttc
//
LOCUS       DASUGB010000572         5328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1037364, whole genome shotgun sequence.
ACCESSION   DASUGB010000572 DASUGB010000000
VERSION     DASUGB010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037364"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFV87_10925"
     CDS             <1..695
                     /locus_tag="VFV87_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5104316.1"
                     /translation="GERPTHPELLDWLAQELIRGGWKLKPLHKQMMLSAAYMQSGEFD
                     EADAKLDPENTWLWRREPRRLEAELIRDSMLAVSGTLDPAMYGPGTLDEGHKRRSIYF
                     MIKRSKLVPMMQLFDQPEPLVSAGSRPSTTIAPQALAFLNSPHVRTAAHAFAQRLLPA
                     AENSPAEAVRQGYLASIARQPDESELSAATSFLAAQEQRYADEQKPNAKELALADFCQ
                     VLFGLNEFVYVE"
     gene            700..2172
                     /locus_tag="VFV87_10930"
     CDS             700..2172
                     /locus_tag="VFV87_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104317.1"
                     /translation="MAPPMLSRRQLLAQAGGGAGMLALASLLADEGLLIGSAGAAESA
                     AGSAAADPRLNPLAPKKSHFPAKAKSVIWLFMNGGPSQVDTWDYKPELEKRDGKALEG
                     FDKNTGFFTGQVGPVMKSPFKFARHGKCGAWVSEIFPRMAEHVDEMAFLYSCYTGSNN
                     HSPALFMINTGTTRMGFPSAGSWVTYGLGSESLSLPAFVAMTDPLGRGLPKGYSQNWS
                     AGFLPSVYQGTWLKPQGDPIDNLYRPADQGEAQQRAALDLLGQLNQHALERSPQELEL
                     AARIESFELAYRMQLAAPEAIDINDEPESIHKLYGIGDKRCDHFAKQCLIARRMVERG
                     VRFVQIYSGGMENERSWDGHQNIKANHEQFAGETDTPIGGLLADLKQRGLLDSTLVIW
                     GGEFGRLPIVQTGGTGRDHNPHAFTYWLAGGGVKGGTLYGATDELGHKAVENRVHVND
                     LHATILHLLGLDHTKLTFRLSGRDFRLTDVAGHVVQEILA"
     gene            2184..2630
                     /locus_tag="VFV87_10935"
     CDS             2184..2630
                     /locus_tag="VFV87_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104318.1"
                     /translation="MPIPALNEHGLLPPGTHDCTLAEIEARFGQFQRSDRRCVLFSRL
                     ASFIREVGKVEFAIAVVIDGSFVTAKDEPNDIDLVLILSESHDFAAELRPFEYNIVSK
                     KRILREYGFDIVIDGAGTVAMRKHLDFFSRVRERADILKGLLRVMI"
     gene            2627..2842
                     /locus_tag="VFV87_10940"
     CDS             2627..2842
                     /locus_tag="VFV87_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104319.1"
                     /translation="MIETHEGLLLSIEQMARMYCALADLHTRVAPLNFRNYQVFAEGP
                     IDEIRKLQREIDEYLGIAEPVTAVNRS"
     gene            2855..3247
                     /locus_tag="VFV87_10945"
     CDS             2855..3247
                     /locus_tag="VFV87_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104320.1"
                     /translation="MNSNGLIVDEHPNPYEAPRVAGNPSHESGQANAFRWWQFALILC
                     LAIGLNGLRLVMTWEAYGLDGYEQVGWPWVFYERGGFSYGEYFYPWLIAADVAVAIGL
                     AYFGGRFTLRDVIQAIRRFQSWGTPQSP"
     gene            3355..4767
                     /locus_tag="VFV87_10950"
     CDS             3355..4767
                     /locus_tag="VFV87_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5104321.1"
                     /translation="MIRSLLVVTAWVFLLNGASAAERLPNFVIVLADDIGSKELACYG
                     NTQHATPRLDQLAKEGMRFETCWATPLCTPTRTMLMTGQYAFRTGYFQMFGSPLSPRP
                     DSSEFQHGDKLTFADVLKAQGYATGLAGKWQLSGEHPTLIHDCGFDTYRMWAYEHNLP
                     EGVKHTGRYEGGNRTARYWHPCIVENGKYVPTQPDDYGPDLFNAFAIDFIRANQDRPF
                     CFYYPMPLTHGPHEETPDSAVVRDSSANATRKRLPAGYKSNVEYLDYLMGQLVDAIDA
                     AGLKENTVFIFVGDNGTAGSGKGQVTELGVRVPLIIRCPGTVQAGVVSRELVSVADIF
                     PTVKELGGGKLPGGHVIDGKSLVPTLRGEKVKHRDWLFSYLGRSRILRNERYVLEVPG
                     QGQPGRLFDASESRTGQGYKDVTGSDDADHAQTLQQFTLLLKELPGPEGHPGLRLSED
                     AGGKGKAKGKKAKQKAKANQ"
     gene            complement(4776..>5328)
                     /locus_tag="VFV87_10955"
     CDS             complement(4776..>5328)
                     /locus_tag="VFV87_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5104322.1"
                     /translation="VPATEYHFIARVLDMGAMGIMAPMVESAEQAQRLVAAVKYPPVG
                     RRGAAFGVSHDDYTGGDLIAKIESANRETVVFAQIETARGIEHVEAIAAVPGIDVLWI
                     GQFDLSISLGIPGQFTHPRLDEATGQVLAACRKHGKSAGIMATDVASGQAMLDQGFGI
                     MAYGGDLWIYQAALRQGIATLRG"
BASE COUNT         1002 a         1644 c         1707 g          975 t
ORIGIN      
        1 agggcgagcg acccacgcat cccgaactcc tcgattggct cgcccaggag ctgatccgcg
       61 gcggctggaa gctgaagccg ctgcacaagc aaatgatgct ctcggcggcc tacatgcaga
      121 gcggcgagtt tgacgaggcc gacgcgaagc tggacccgga aaacacgtgg ctttggcggc
      181 gcgagccgcg gcgcctggag gcggaattga ttcgcgacag catgctcgcg gtcagcggta
      241 cgctcgaccc tgcgatgtac ggccccggca cgctcgacga agggcacaag cgccgcagca
      301 tctacttcat gatcaagcgc agcaagctcg tgccgatgat gcagctcttc gatcagcctg
      361 agcccttggt cagcgccggc agccggccca gcacgacgat cgccccgcag gcgctggcct
      421 tcctcaatag cccgcacgtc cgcaccgcgg cccacgcctt tgcccagcgg ctgttgcccg
      481 ccgccgaaaa ttcgcccgcc gaagccgtcc gccaaggtta cctcgcttca atcgcccgac
      541 agccggacga aagcgagcta tccgccgcca ccagcttcct tgccgcccaa gagcagcgct
      601 acgccgacga gcaaaagccc aatgccaaag agctggcgct ggccgatttt tgccaggtgc
      661 tgtttggttt gaacgagttt gtgtatgtgg agtgacaaca tggccccccc aatgctttcc
      721 cgtcgtcagc ttcttgctca ggccggaggc ggcgccggga tgctcgcttt ggcttcgctg
      781 ttggccgatg aggggctgct gatcggctcc gcgggagccg cggagagcgc ggcaggctcg
      841 gcagcggccg acccgcggct gaatccgctg gcgcccaaga agagccattt cccggcgaag
      901 gccaagagtg tcatctggct gttcatgaac ggcgggccga gccaggtgga tacgtgggac
      961 tacaagccgg agttggagaa gcgcgacggc aaggcgctgg aaggcttcga caagaacacg
     1021 ggatttttca caggccaggt cggtccggtg atgaaatcgc cgttcaagtt tgcccggcac
     1081 gggaaatgtg gggcctgggt cagcgaaatc ttcccgcgga tggccgagca cgtcgatgag
     1141 atggcctttc tctattcgtg ttacaccggc agcaacaatc attcgcccgc cctgttcatg
     1201 atcaacacgg ggacgacgcg gatgggcttt ccctccgccg gctcgtgggt gacgtacggc
     1261 ctgggaagcg aaagcctcag cctgccggcg ttcgtggcga tgaccgaccc gctcggccgc
     1321 ggtctgccta agggctattc gcagaactgg agcgcgggct ttctgccgag cgtctatcag
     1381 ggaacctggc tcaagccgca gggcgatccg atcgacaacc tctatcgccc tgccgaccag
     1441 ggcgaggccc agcagcgggc ggctctcgac ttgctcggcc agctcaacca gcacgccctg
     1501 gagcgctcgc cgcaagaatt ggagctggcg gcccggatcg agagcttcga gctggcctac
     1561 cgcatgcagc tcgcggcccc cgaagcgatc gacatcaacg acgagccgga atcgatccac
     1621 aagctctacg gcatcggcga caagcggtgc gaccattttg cgaagcagtg cctgatcgcc
     1681 cggcggatgg tcgagcgtgg cgtacggttc gtgcagattt atagcggcgg gatggagaat
     1741 gagcgatcct gggacggcca tcagaatatc aaggccaacc acgagcagtt cgccggcgaa
     1801 accgacacgc cgatcggcgg cctgttggcc gacctcaagc agcgcgggct cctcgacagc
     1861 acgctcgtta tctggggagg cgaattcggc cgcctgccga tcgtgcagac cggcggtacc
     1921 ggccgcgacc acaatccgca cgccttcacc tactggctgg ccggcggcgg cgtgaaaggg
     1981 ggcacgctct atggcgcgac cgacgagctc ggccacaaag cggtcgaaaa ccgcgtccac
     2041 gtcaacgacc tgcacgccac cattctgcac ctgttaggcc tcgaccacac caagctgacc
     2101 ttccgcctct ccggccgcga cttccggctg acggacgtcg ccgggcatgt ggtacaggaa
     2161 atcttggcgt aggcgccatt tccatgccga ttcccgcgct caacgaacac ggcctgttgc
     2221 cgccgggcac gcacgattgc actctggccg agattgaggc gcgctttggt cagtttcagc
     2281 ggtcggatcg acggtgcgtc cttttctcga ggttggcgag ttttattcgc gaagttggca
     2341 aggtggagtt tgccatcgca gtcgtcatcg acggaagctt tgtcacggcg aaagacgagc
     2401 caaacgacat tgatcttgta ctcatcttgt ccgaaagcca tgattttgcg gccgagttgc
     2461 ggccctttga gtacaatatc gtgtcgaaga agcgaattct tcgcgagtat ggattcgaca
     2521 tcgtcatcga cggtgccgga accgtggcca tgaggaaaca tttggatttt ttttcccggg
     2581 tgcgagaacg cgcggacata ttgaaagggt tgttgagggt gatgatatga tcgaaactca
     2641 tgaaggcctg ttgctgtcga tcgagcagat ggcgcgaatg tactgtgctc ttgccgatct
     2701 acacacgcgc gtcgctcccc tcaacttccg caattatcag gtctttgcgg aaggtcccat
     2761 cgacgaaatc cgcaaattgc agcgtgagat cgacgagtac ttgggcattg ccgaaccggt
     2821 caccgcggtc aaccggtcgt aatcgacttt ctgcttgaac tcgaatggcc tgatcgtgga
     2881 cgagcatccc aacccgtatg aggcgccgcg cgttgcaggg aatccatcgc atgaatcagg
     2941 acaagccaat gcttttcgtt ggtggcagtt cgcgttgata ctgtgcttgg cgatagggct
     3001 caacggtctg cggttggtga tgacgtggga ggcgtatgga ctggatggct acgaacaagt
     3061 tggctggccc tgggtgttct acgagcgcgg cggcttcagt tacggcgagt atttctatcc
     3121 ctggctgatt gcagccgacg tcgccgtcgc gattggactg gcctatttcg gaggtcgctt
     3181 cacgctgcga gacgtgattc aggcgattcg acggtttcaa tcttggggta caccgcaatc
     3241 gccgtaagtc atggccaacg gaagcaaagg aaatcctcgc caagccgatg cggatcgact
     3301 aggatgcgcg cctgctcgcg gtggtcctgt gcgcatgtcg tgggagattc ttcgatgatc
     3361 cgctcgcttc tcgtcgtgac ggcttgggtg ttcttgctga atggggcctc cgctgccgag
     3421 cggctgccga actttgtgat cgtgctggct gacgacattg ggtccaagga gctggcctgc
     3481 tacggcaata cgcagcacgc cacaccgcgg ctggaccagc tcgccaagga ggggatgcga
     3541 ttcgaaacct gctgggccac gcccctctgc acgcccacgc gaaccatgct gatgaccggg
     3601 cagtatgcct tccgcacggg gtattttcag atgttcggca gcccgctgtc gccgcggccg
     3661 gactcgtccg agtttcagca tggcgacaag ctgacttttg ccgatgtgct gaaggctcag
     3721 ggctacgcca ccggcctggc cggcaaatgg caactctcgg gcgagcatcc cacgctgatc
     3781 cacgactgcg gcttcgatac gtaccgcatg tgggcgtacg agcacaacct gccggagggc
     3841 gtcaagcaca ccggccgcta cgagggaggc aatcgcaccg cacgctactg gcacccgtgc
     3901 atcgtcgaga acggcaaata cgtgccgacg cagccggacg attatggtcc cgatctgttc
     3961 aacgcctttg cgatcgactt cattcgcgcc aaccaagaca ggccgttctg cttttactac
     4021 ccaatgccgc tgacgcatgg cccgcacgag gagacacccg attccgccgt tgttcgcgat
     4081 tccagcgcga atgcgacccg caagcgcctg ccggctggtt acaagtccaa tgtcgaatac
     4141 ctggactacc tcatgggcca actcgtcgat gcgattgatg cggcaggtct caaggaaaac
     4201 accgtcttca tctttgtcgg cgataacggt accgccggca gcgggaaggg gcaggttacg
     4261 gagctcggcg tccgcgtgcc gctcatcatc cgctgtccgg gaaccgttca ggccggcgtc
     4321 gtcagtcgcg aattggtcag cgtggccgac atctttccca ccgtgaagga gctgggcggt
     4381 ggcaagctgc ctgggggcca cgtgatcgac ggcaagagcc tcgtccccac cctccgcggc
     4441 gagaaggtca agcaccgcga ctggctcttc agctatctcg ggcgaagccg catcctccgc
     4501 aatgagcgct acgttctgga agtgcccggc caggggcagc ccggccgtct gttcgacgcc
     4561 agcgaatcgc gcacgggcca gggatacaaa gacgtcaccg gctccgatga cgcggatcat
     4621 gcgcaaacgc tgcaacagtt cacgctgctg ctcaaggagt tgcccggtcc ggaaggccac
     4681 cccggcctgc gcctgtccga agacgcgggc ggcaaaggca aggccaaagg caaaaaggcg
     4741 aagcagaagg ccaaggcgaa tcagtaggct gccttctagc cgcggagcgt cgcgatgcct
     4801 tgccgcaggg cggcctgata gatccagagg tcgccgccat aggccatgat gccgaagccc
     4861 tggtcgagca tggcctggcc gctggcgacg tcggtggcca tgatgccggc ggacttgccg
     4921 tgcttgcggc aggcggcgag aacctggccc gtcgcttcgt ccaggcgcgg gtgcgtgaac
     4981 tggcccggaa tgccgaggga gatcgagagg tcgaactggc cgatccagag gacgtcgatg
     5041 ccggggacgg cggcgatggc ctccacgtgc tcgatgccgc gggccgtttc gatttgggcg
     5101 aagacgactg tctcgcggtt ggccgattcg atcttggcga tcaggtcgcc gccggtgtag
     5161 tcgtcgtggg agacgccgaa ggcggcgccg cggcggccga cgggcggata cttgaccgcc
     5221 gctaccagcc gctgggcctg ctcggccgat tcgaccatcg gggccatgat gcccatcgcc
     5281 cccatgtcga gcacgcgggc gatgaagtgg tattcagtcg ccgggacg
//
LOCUS       DASUGB010000573         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_63744,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000573 DASUGB010000000
VERSION     DASUGB010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63744"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFV87_10960"
     CDS             complement(<1..318)
                     /locus_tag="VFV87_10960"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5104323.1"
                     /translation="MGEPALPIFVYGTLQRGPCRERCWPRQPLRVESARVRAALYDLG
                     PYPALVASDDKIAEELWQLAEGDLPVTLAELDRVEGYAGQIDDLYRRATISCQTASGS
                     VEAW"
     gene            364..996
                     /locus_tag="VFV87_10965"
     CDS             364..996
                     /locus_tag="VFV87_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX5104324.1"
                     /translation="MNADPNPYASPAVESPPLVTAVVVEDAGWTIDYELRIDDYIVWN
                     MHFHRTSHVARRQAMLAWVLTGFLILMIALLLPSLALDWPESIVTSAVIAIPGVALFC
                     LFPVLRRWQLQRVIRRMYVEGHNYNLIGPRRLAISGEYVSVSSPWSHSLMRWAGIAEI
                     RREPEAVYFCISSNTAYIVPRRAFAGAEQFQQFAQAAADFHAKAKPAVVA"
     gene            1139..1408
                     /locus_tag="VFV87_10970"
     CDS             1139..1408
                     /locus_tag="VFV87_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5104325.1"
                     /translation="MRKFLTLAVVAALFTPAVLSAQDKKKPDPEAQFAKLDANNDKKL
                     SLEEFKGKRTGDKADKAGEQFKKMDKDSDGFLTLEEFKARGKKAK"
     gene            complement(1459..1953)
                     /locus_tag="VFV87_10975"
     CDS             complement(1459..1953)
                     /locus_tag="VFV87_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5104326.1"
                     /translation="MKTLLLLRHAKSSWKDGDLDDHDRPLNKRGKRDAPRMGRLLADE
                     RLLPDLILCSTAKRCRKTAEHVIAASGYRGDTRITGELYEADGANLRESLAGVSEQPA
                     SILLIAHNPGMEELLESLTGLYTPLTTAALAQVEMPIDHWRDLTDQTRGRLIRLWQPR
                     ELES"
     gene            complement(1950..>2888)
                     /locus_tag="VFV87_10980"
     CDS             complement(1950..>2888)
                     /locus_tag="VFV87_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX5104327.1"
                     /translation="IVELKARFDEARNIEWARNLEENGVQVIYGVKGLKTHAKVCIIV
                     RREPHGVVRYVHFGTGNYNEITARIYSDASLLTSNEELGADAVSFFNAITGYSQPQRY
                     RKIEAAPTGLRQRILELIEAETHRQQQGQKAFIDVKVNSLVDPEIITALYAASQAGVP
                     VRLNVRGVCCLRPGVAGLSENISVVSIVDRYLEHARILHFHHGGDGLVFLSSADWMPR
                     NLDRRIELLVPVEDPPLKSRLINILEAYFDDNVKARHLQADGSYARGKGKGRKKPRRS
                     QELLYEDARRRVKEAEQSQQTTFEPHRAPSEEDRAR"
BASE COUNT          532 a          865 c          891 g          600 t
ORIGIN      
        1 ccacgcttcc acggagccac tcgcagtctg gcacgagatc gtggcgcgac gatataagtc
       61 gtcgatttga ccggcataac cttcaacacg atccagctcg gccagggtga ctggcaggtc
      121 gccctcagcc agttgccaca actcttcggc aatcttgtcg tcgcttgcga caagcgccgg
      181 atacggcccc aggtcgtaga gcgccgcgcg cactctcgcc gactccacgc gcagcggctg
      241 tcgcggccag cagcgctcgc ggcacggtcc gcgttgcagc gtgccataga cgaaaattgg
      301 aagtgccggt tcgcccatat actgtcttac aatgtcttct ccaacatcgc tgcctgacct
      361 gtcatgaatg ctgatccgaa tccctacgcc tcgcccgccg ttgaatcacc gccgctcgtg
      421 acggctgtcg tggtcgagga cgccggttgg acgatcgact atgaactgag gatcgacgac
      481 tacatcgtgt ggaacatgca tttccatcgg acgtcgcatg tcgcacgccg ccaggcgatg
      541 ctggcttggg tactgaccgg attcctgatc ctgatgatcg cgctgttgtt gccttcgctg
      601 gccctggatt ggccggaatc gattgtgaca agcgccgtga ttgcgattcc cggcgtggca
      661 ttgttttgtt tgtttcccgt gctccgtcgc tggcaattgc agcgcgtcat ccgcaggatg
      721 tacgtggaag ggcacaatta caatctgatc ggccctcggc gactggccat ttctggcgag
      781 tatgtctcgg tttcgtcgcc gtggtcgcac tcgctcatgc gctgggcggg gatcgccgaa
      841 atccgccgag agccagaagc ggtctatttc tgcatttcgt ccaacacggc gtacatcgtt
      901 cccaggcgag cattcgcggg cgccgagcag ttccagcagt ttgcccaggc cgctgccgac
      961 tttcatgcga aagccaaacc agcggttgtc gcataaacgc gccgcgatgt tgcacgatcg
     1021 gagcaaggag caaatcatcg cattacaaga atgtaaggca aataattgtg ccgcctggcg
     1081 catcggacat tcaacgggca attgccctca atacatctct ttgctgagga gcgttcgcat
     1141 gcggaagttt ttgacattgg ccgtggtggc cgcgctgttt acgccggctg tcctctcggc
     1201 tcaggacaag aaaaagcctg atccggaagc ccaattcgcc aagctcgacg cgaacaacga
     1261 taagaagctg agcctggagg agttcaaggg caagcgcacg ggcgacaaag cagacaaagc
     1321 cggtgagcag ttcaaaaaaa tggacaagga cagcgacggc tttttgacgc tggaggaatt
     1381 caaggcccgc ggcaaaaagg ccaagtagga ttgcgtcaat cggcccggtc ttagttcgtc
     1441 caccaagggc ggctgacctc acgattccag ctcccgcggc tgccagagcc tgatcaggcg
     1501 gccgcgggtc tggtcggtga gatctcgcca gtggtcgatg ggcatttcca cttgcgccaa
     1561 agccgcagtc gtgagcgggg tatagaggcc ggtcaacgat tcgagcagct cttccattcc
     1621 gggattgtgg gcgatcaata ggatgctggc aggctgctcc gagacgccgg ccagcgattc
     1681 gcggaggttt gcgccatctg cttcgtacag ctcgccggta atccgcgtat cgccgcggta
     1741 gccgctggcg gcgatgacgt gctcagccgt cttgcggcag cgtttggcgg tcgagcacag
     1801 aatcagatcc ggtagcaggc gctcatcggc cagcagccgt cccatccgcg gtgcatcgcg
     1861 tttgccgcgc ttgttgagcg gccggtcgtg gtcgtccaag tccccgtctt tccagctcga
     1921 cttggcgtgc cgcaagagaa gcagcgtctt catcgcgccc ggtcctcctc gctgggagct
     1981 cgatgcggtt caaaggtcgt ctgctgggat tgctcggctt ccttcacgcg gcggcgggcg
     2041 tcttcatata gcagctcctg gctgcgacgg ggcttcttgc ggcctttacc cttgccgcga
     2101 gcataagagc cgtcggcctg caggtgacgg gctttgacgt tgtcgtcgaa ataggcttcc
     2161 aggatgttga tcaggcggct cttcagcggc ggatcctcga ccggcacgag cagctcgatg
     2221 cggcggtcga ggttccgcgg catccagtcg gcgctagata gaaacacgag tccatcgccg
     2281 ccgtggtgga agtgcaggat acgagcatgc tccagatatc gatcgacgat gctcacgacg
     2341 ctgatgttct cgctcaaacc tgccacgccc ggccgcaagc agcagacacc ccgcacgttc
     2401 agccgcaccg gcacgccagc ctgtgacgcg gcatagaggg cagtgatgat ctctggatcg
     2461 acgagcgaat tgaccttcac gtcgatgaag gccttttgcc cttgctgctg gcggtgggtc
     2521 tcggcctcga tcagctccag aatccgctgc cgcaggccgg tcggcgcggc ctcgattttg
     2581 cggtagcgct gcggctgcga atagccggtg atggcgttaa agaagctgac ggcatcggcc
     2641 cccagctctt cattgctggt gagcaggctg gcatcgctgt agatgcgggc ggtgatttcg
     2701 ttgtagttgc cggtgccgaa atgaacgtag cgcacaacgc catgcggctc gcggcggacg
     2761 atgatgcaga ccttggcgtg cgtcttcagg cccttgacgc cgtagatgac ctgcacgccg
     2821 ttctcttcta gattgcgggc ccattcgatg ttgcgggcct cgtcgaaacg ggctttcagc
     2881 tcgacgat
//
LOCUS       DASUGB010000574         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_619716, whole genome shotgun sequence.
ACCESSION   DASUGB010000574 DASUGB010000000
VERSION     DASUGB010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619716"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFV87_10985"
     CDS             <1..945
                     /locus_tag="VFV87_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104328.1"
                     /translation="RIRMTGVAIPLKRRWFQFSLRTLLVATAFVGLVLAPIVYYRQLA
                     HRQQQIKAELEVHRIVLSGGKEQSQRPQWLKKILGDDSAGLDICIHVYAASLLESSES
                     RERAIDKLNQLPSATMLVFPDQIDDQGLVQFRRLTNIQTLFLERSPITSDGLKHFRGC
                     QDLGVLNLVATPIGNRDLKEIARHKNLEILYLWGTNVTDEGMQELRPLKRLRCLHLDK
                     TRVTDAGLRELQQFQSLEMLGFGVTSVSDAGIRELRPLKNLTHLDLGGTKVTDECLSE
                     LEYHPNLQSVNLENTAVTLTGIRKLKAKLPSLRVNRNL"
     gene            1043..2581
                     /locus_tag="VFV87_10990"
     CDS             1043..2581
                     /locus_tag="VFV87_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5104329.1"
                     /translation="MLAKLQTFSLLGIEAVPVEVEVDVSPGALPKIFLVGLPEAAVKE
                     SQYRVERAMANSGYLRPQDRVVINLAPAELPKSAAAFDLPITLGMLVASGQLESERLE
                     QYAVVGELSLEGTTRPTKGALSMAMAAARQRNLRGLVVPAESAAEAAVVEEIEVIPVT
                     SLTQAVGFFAGQLEIEPTPSRIGELFDSFAKYEEDFGDVRGQEMAKRAIVVAAAGKHN
                     LLMVGPPGSGKTMLAKRMPTILPSLQAGESIETTRIYSSLGRLQPGQPLMARRPFRSP
                     HHTISDAGLVGGGNPPVPGEISLSHNGVLFLDELPEFNRRTLEVLRQPLEDATVTISR
                     ALHSTTFPADFMLVAAFNPCPCGYRGDPRRDCHCSTPQIERYMGKISGPLLDRIDIHI
                     EVPAVPFKELSAERGGTSSADMRGLVVAARAIQAQRFAGSRTRYNAQMSSRQIRNFCK
                     LTDECQDLLKDSVNNLGLSARAHDKVLRVARTIADLDNSDNITAGHINEAINYRMLDR
                     QMWT"
     gene            2633..>3065
                     /locus_tag="VFV87_10995"
     CDS             2633..>3065
                     /locus_tag="VFV87_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX5104330.1"
                     /translation="MSLTVLLIILAMLLLNGIFAAYELALASVRAERLKHMVEQRRRG
                     AVIALRMKNRMEASLAVVQLGITLVGAIAAATGGADAKDKLVPLIKDQLGLSPHLANI
                     VALTCLVIPLAAITIIVGELIPKVFALKNSELVVTLLSPLMW"
BASE COUNT          614 a          961 c          898 g          592 t
ORIGIN      
        1 cgcattagaa tgacgggcgt ggccattccg ctcaaacgcc gctggtttca gttcagcctc
       61 aggacgctgc tggtcgctac ggcgtttgtt gggctcgtgc tcgcccccat cgtctattat
      121 cgccagctcg ctcaccggca gcagcagatc aaagcggagc tagaagtgca ccggattgtg
      181 ctcagcggcg ggaaggagca atctcagagg ccacagtggc tgaaaaaaat cttgggggat
      241 gactctgcgg ggctcgacat ctgcattcat gtctatgccg cttcactttt ggagtcgtca
      301 gagtctcgtg agcgagcaat cgataagtta aatcaattac ccagcgctac gatgcttgtt
      361 ttcccggacc agatcgacga tcagggattg gtgcagtttc gacgattgac caatatccaa
      421 actctctttc tggaacggtc gccgattacg agtgacggcc taaagcactt tcgcggatgc
      481 caggatctgg gtgtgcttaa cctggtagct acaccaatcg gcaacagaga cctcaaagaa
      541 attgcccgac ataaaaattt agaaattctt tacctttggg gaacgaacgt gaccgatgaa
      601 ggcatgcaag aactgcggcc gcttaagagg ttgaggtgcc tccatctaga caagacacgt
      661 gtcacggatg cgggcctcag ggaactccag caattccaat cattggaaat gcttggtttt
      721 ggggtaactt ccgtttcaga tgcgggaatt cgagaattgc gaccgctcaa aaacctaact
      781 cacctcgatc tgggagggac caaagtcact gacgagtgtc tttcggaatt ggaataccac
      841 ccgaatcttc aaagcgtcaa cctggagaac acggcggtta cgctcacggg gatccggaag
      901 ctcaaagcaa aattgccgag tctgagggtc aatcgtaacc tgtaagcctt ggcaatcgag
      961 ttctgtaact tgaatctctc tcgccggacc atcacaatgg cggtgcccta gctccctttg
     1021 ctttcgcacc tcaggtcctc ccatgcttgc caagctccag acattttccc tgctggggat
     1081 tgaggcggtg ccggtcgagg tggaggtcga tgtctcgccg ggggcgctgc cgaagatttt
     1141 tctggtcgga ctccccgagg cggctgtcaa ggagagccag taccgggtcg agcgggcgat
     1201 ggccaacagc ggctatttgc ggccgcagga ccgggtggtg atcaacctgg ccccggcgga
     1261 gttgcccaag agtgcggctg cgttcgatct gccgatcacg ctcgggatgc tcgtcgccag
     1321 cgggcaattg gaatcggagc gattggagca gtatgccgtc gtcggagagt tgtcgctcga
     1381 agggactacg cggccgacga agggggcgct gtcgatggcg atggcggcgg cccggcagcg
     1441 gaatttgcgc gggctggtgg ttccggcgga aagtgccgcc gaagcagccg tcgtcgagga
     1501 gattgaagtc attccggtga cgagcctgac gcaagcggtc gggttcttcg ccgggcagct
     1561 cgaaatcgag ccgaccccca gccgcatcgg cgagctgttc gattcgttcg ccaaatacga
     1621 ggaagacttc ggcgacgtcc ggggtcagga aatggccaag cgggccatcg tcgtcgctgc
     1681 ggctggcaag cacaatctgc tcatggtcgg gccgcccggt tccggcaaga cgatgctggc
     1741 caagcggatg ccgacgattc ttccctccct gcaagccggc gagtcgattg aaacgacccg
     1801 catctacagc tccctcggcc gcctgcaacc gggtcagccg ctcatggccc ggcggccgtt
     1861 ccgctcgccg catcacacga tcagcgacgc cggcctggtc gggggcggca atccgcccgt
     1921 gccgggggaa atctcgctgt cgcacaacgg cgttttgttc cttgacgaac tacccgaatt
     1981 caaccgtcgc acgctcgaag tcctccgcca gccgctggaa gacgcgacgg tgacgatttc
     2041 ccgagcactc cattccacga cattccccgc cgacttcatg ctcgtcgccg ccttcaaccc
     2101 ctgcccctgc ggctatcggg gggacccgcg gcgggattgc cattgcagca cgccgcagat
     2161 cgaacggtac atgggcaaaa tctccggccc actgctcgac cggatcgaca tccacatcga
     2221 agtccccgcg gtcccgttca aggagctttc cgccgagcgc ggcggcacca gcagcgccga
     2281 catgcggggc ctcgtcgtcg ccgcccgcgc catccaagcc cagcgcttcg ccggcagcag
     2341 aacgcgatat aacgcccaga tgtcgtcgcg gcaaatccgc aacttttgca agctcacgga
     2401 tgaatgccag gacctgctca aagacagcgt caacaatctg ggcctctccg cccgcgccca
     2461 cgacaaagtc ctccgcgtcg cccggaccat cgccgatctc gacaacagcg ataatatcac
     2521 cgccggccac atcaacgaag ccatcaatta ccggatgctc gaccggcaga tgtggacgtg
     2581 acggtccgtc cctcgcctcg ccgattgcca gcgcgactgg tattctgctg gcatgtctct
     2641 cacggtcctg ctcatcattc tcgccatgct cctgctcaac ggcatcttcg ccgcctatga
     2701 gttggccctg gcctccgtcc gcgcggaacg cctgaagcac atggtcgaac aacgccgccg
     2761 cggggcggtc atcgccctgc ggatgaagaa tcggatggag gccagcctgg ccgtggtgca
     2821 gctcggcatc acgctcgttg gagccatcgc cgcggccacc ggcggagccg acgcgaagga
     2881 caagctggtc ccgctcatca aggatcagct ggggctttcc cctcacctgg caaacatcgt
     2941 agccctcacc tgccttgtga tccccctcgc cgcaatcacc atcatcgtcg gcgaactgat
     3001 tcccaaggtc ttcgccctca agaacagcga gttggtggtc acgctcctca gcccgctgat
     3061 gtggg
//
LOCUS       DASUGB010000575         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_759674, whole genome shotgun sequence.
ACCESSION   DASUGB010000575 DASUGB010000000
VERSION     DASUGB010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759674"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFV87_11000"
     CDS             <1..1351
                     /locus_tag="VFV87_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX5104331.1"
                     /translation="QGWDIKGTWNVGCPVPSVTPAQPEVPPVAGELTLLIRDNPAVCA
                     WSADPGAQFLTATSAVTGMGDGEVSYAYPPNTSATSRLAQLTVGGRPVDITQLGAGQA
                     PDDEVVYYHQDAIGSVRLVTDALGQQLRRHDFAPFGIEVGPSSSNEGFMFAGNQRDGF
                     GFDYLNARYFAPATDRFTSPDPLSITNARLFIPSRLNRYTYGNNNPLSFVDPTGLEPC
                     QEVHMLEDGTTTPVYDCNTYTYNLCDFDCSLWRWSLWTPISYEPPTPPTIPAAPPTPP
                     TPPMRPRCADVPSEVSSFNYGAQIGTGGLFGLYGNIGINWTTGQLILGGQFTVTKGGG
                     SYVGYGPGVTLLQSGGGIPSWQLNVSDWTEYYEVVGGWKGSAGLSLTGTRTGKSPRQA
                     REARGEAMPRLDSITVSARGGAGYGFVMGSGGSRTASVATSGWLGIPELLGLDYGCR"
     gene            complement(1937..2647)
                     /locus_tag="VFV87_11005"
     CDS             complement(1937..2647)
                     /locus_tag="VFV87_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104332.1"
                     /translation="MFFNRNGLPASYAYVVPPGKAVPAELLSQSQGPAMLLMAAHALL
                     PSARSPLGVEHGAMVCADWPGHGTPVRPDYIFSALDIAETMTFHGLIALLWGSQTAGG
                     IDESGRGPAQPFVTQLAQRLLAGGPNGALAVVGLTDRLLGASLGPSAVSPRQALTLAI
                     RGLARGQRVGHAMQSLDLAYSAMAAELAEIVEGVRSGLQMDPVALAQLGAATMDLRRV
                     VVLGDPAVGVPAALAGSR"
     gene            2658..>2868
                     /locus_tag="VFV87_11010"
     CDS             2658..>2868
                     /locus_tag="VFV87_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104333.1"
                     /translation="MTDFDYDSNGNLKSGPAVGSGTFTYTYRADNMMSGATVGSTVTG
                     FAYDGGASRIKKTTGGVPTYFLRGPD"
BASE COUNT          532 a          850 c          900 g          586 t
ORIGIN      
        1 acaaggatgg gacatcaagg ggacgtggaa cgtcggttgt ccagttcctt cggtgacgcc
       61 tgcgcagccg gaagtgccgc cggtggccgg ggagctgacg ctgctaattc gcgacaatcc
      121 ggcggtctgc gcgtggagcg ctgaccccgg cgcgcagttc ctgacggcta cctcagcggt
      181 cactggaatg ggggatggag aggttagcta tgcgtacccg ccgaacacga gcgcgacttc
      241 ccgtctcgcg cagcttaccg taggcgggcg gcccgtggat atcacgcaac tcggcgccgg
      301 acaggccccc gacgacgagg tggtgtacta ccaccaggac gcaattggtt cagtgcggct
      361 cgtcacagat gcgctcgggc agcaactccg ccggcacgat tttgcgccgt tcggcatcga
      421 agtcggaccg tccagttcca atgaaggttt catgttcgcc ggcaatcaac gcgatggatt
      481 cgggttcgat taccttaacg ctcgctactt tgcgccggcg acggaccggt tcacctcgcc
      541 agatcccctt tcaattacca atgccagact cttcattccg agtcggctca atcgatatac
      601 gtacggcaac aacaatcctc tatcattcgt ggatcccact ggcttggagc catgtcagga
      661 agtccatatg cttgaggacg gaacgacgac gccggtgtac gactgcaaca cttacaccta
      721 taacctctgc gattttgact gctccctttg gcgttggtcg ctttggaccc cgatcagtta
      781 cgaaccgcct actccgccca cgattcccgc agcgccccca acgcctccga cgccgccgat
      841 gaggccgcga tgcgccgatg tgccttctga agtttctagt ttcaattatg gtgctcaaat
      901 cggaactgga ggtctgttcg gtttgtatgg aaatatcggg ataaactgga cgactggtca
      961 actcattctt ggtggacagt ttacggttac gaagggcggt ggctcgtacg tcgggtacgg
     1021 ccctggcgta actcttctcc aaagtggggg cggcattcca agctggcaac tcaatgtgtc
     1081 agattggacc gaatactatg aggtcgtggg aggctggaag ggatcggctg gattgtcgct
     1141 tactggaacg cgtactggaa aatcgccgcg gcaagcgaga gaagctaggg gagaagctat
     1201 gcccaggctc gattcaataa ccgtatctgc tcgcggtgga gccggctatg gctttgtcat
     1261 gggcagcggc ggatcgcgca ctgcgagcgt cgcaacgtcg gggtggttgg gaattcctga
     1321 gttgttggga ctggactatg ggtgccggta agaagatgga tctgtttgcg gcgctgacca
     1381 tttttagttt ggtggctcta gtcgctctga ctacggcaga attcgtgcct ttacgtttag
     1441 gcttgttgag cgtagccagc attgcttggg tctactttcg gtttcgtttg gcgaaatggt
     1501 accatgccag ctttcatgta gcatggagac tgacaggcct tattgcaata gttgggtgtg
     1561 taacatcggt agccacggcg ttcaagcgag aagatcccgt ggtagcgttg atagctgttc
     1621 tggccggcgc ctatggctca tttgcagtaa tggctcggcc gcatcgcgtc gacttggggg
     1681 atcgctcttg gtggcatggg ctgttttacc acgatgctgg tctggagcct attaaacgcc
     1741 ctagaaactg gtggaccggt gggcttcgac cgacgagtcg gtcaagctga gctccccccg
     1801 tgtctatcgc tgccttgtta tttgggcgtt tgagtgaagc gtaccgggtt cgtcggagag
     1861 caagtatgtg agtcaggcca ccttggcctg ctgttaatcg ccagcgccgg gtcgtcacgc
     1921 gatcccgctc gaagcgctat cgtgatccgg cgagggccgc cggcactcct accgccgggt
     1981 cgccaaggac gaccacccgc cgcaggtcca tcgtcgccgc ccccagctgc gcgagcgcca
     2041 cgggatccat ctgcaggccg gaccgcacgc cttcgacgat ttccgccagc tccgccgcca
     2101 tcgccgaata ggccagatcg agcgactgca ttgcgtgccc gacgcgttga ccgcgggcca
     2161 ggccgcggat cgccagcgtc aacgcctggc gcggtgacac ggcgctcggg cccagactcg
     2221 caccgagcag acgatccgtg agtccgacga cggcaagtgc gccgttcgga ccaccagcga
     2281 gcaggcgctg cgcaagctgc gtcacgaatg gctgcgccgg cccgcggccg ctctcatcga
     2341 tgccgccggc cgtctgcgat ccccacagca gcgcgatcag gccatggaat gtcatcgtct
     2401 cggcgatatc gagcgccgaa aaaatatagt ccggccgcac cggtgtgcca tggccgggcc
     2461 agtcggcgca caccatcgct ccgtgttcga ctccgagcgg cgaacgagcc gacggcagca
     2521 aggcatgcgc cgccatcagc agcatcgccg gtccttgtga ctgcgacagc agttccgccg
     2581 gcaccgcctt tccgggtggg accacgtacg cgtacgacgc tggcaatccg tttcggttga
     2641 agaacatcgg cggcgcggtg accgatttcg attacgacag caacgggaac ctgaagagtg
     2701 gcccagccgt cggttcgggc acgttcacct acacataccg ggccgacaac atgatgtcgg
     2761 gcgcgacggt cgggtcgacg gtgacgggct tcgcctatga cggcggggct tcgcgcatca
     2821 agaagacgac gggaggcgtg ccgacgtact ttctccgcgg cccagatg
//
LOCUS       DASUGB010000576         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_204456, whole genome shotgun sequence.
ACCESSION   DASUGB010000576 DASUGB010000000
VERSION     DASUGB010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204456"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFV87_11015"
     CDS             <1..906
                     /locus_tag="VFV87_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104334.1"
                     /translation="LPAVQAAREASRRSQCKNNLKQIGLALQNYHDILGSFPAGWIDT
                     NNTATWECWGWGALLLPYMEQKGLHESLGVDRANLKNILETNGPAAKLLVERPLQAFM
                     CPTDSGFAGRGQVHQNRHFDDGAGTVAGGIAPLRPAVSNYVGVMGHRRVATVTENTGM
                     FYGNSYIRFSDILDGTAHTMAVGERDTRRCRSGAWVGVRNSATGTGSRGVHVVGGRNE
                     PKLNQPDPPIAWDANNGCGEGFSSFHAKGVQFVACDGSVRFLNEDINHFWVGNGANAH
                     TDPLNGVYQRLLTRNDNQPTPGYEQ"
     gene            1026..1436
                     /locus_tag="VFV87_11020"
     CDS             1026..1436
                     /locus_tag="VFV87_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5104335.1"
                     /translation="MPSSSYKRRLALALMLVLLAASSSGCGGSANVAPVKGRVTLDGS
                     PLENALVQFSPGGVGAPSSGRTNAQGEYELTYTEDVKGAVIGNHVVRVTTYQSGDPDA
                     DPPRPEVAERVPEKYAAGIPKEVKPGANVINIEL"
     gene            complement(1472..1960)
                     /locus_tag="VFV87_11025"
     CDS             complement(1472..1960)
                     /locus_tag="VFV87_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5104336.1"
                     /translation="MSTANAPRKHTRRFDSPQQEAFLSLWRTYDRLRMLEDALFSEHD
                     LTAQQYNALRLLKAEHPGSLATLELAGRLVSRAPDITRLVDRLEQRGLVARQRMADNR
                     RVVRIAITSAGLELLRRLEEPVRHCHQRQLGHLSAGELRSLLDLLRTARQPHEESDSA
                     WR"
     gene            complement(2057..2911)
                     /locus_tag="VFV87_11030"
     CDS             complement(2057..2911)
                     /locus_tag="VFV87_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5104337.1"
                     /translation="MTSRADYLRVFATFARNSLVRNMMFRTNFIVECVSSVTWTMMNL
                     GFYLLIFQYTRSIGAGTGWGQWEFFAFLATTMLVNSIVQAFFMPNCEEMSDLIRTGGL
                     DFALLKPIDTQFLISLEKVDWSSLANFAAGLVLLVVSLVQLTTRDTQPLALRSEMVVL
                     YIFYVLCGVAILYSLMIALSATSIWLGQNRSLYDFWFYITNFSRYPMEIYSGRFGTPL
                     QWTCTFIIPVLVVVNVPARILVRPIAPTGESVWPLAAFTLLATAGSLVVSRWIFQAAL
                     RSYRSASS"
     gene            complement(2939..>3651)
                     /locus_tag="VFV87_11035"
     CDS             complement(2939..>3651)
                     /locus_tag="VFV87_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX5104338.1"
                     /translation="PIVTQVFLWYAVFEARKGEALAGYKYEDFVAYYLLTMVARAFSS
                     MPGLASGIAAQIRSGEIKKFLIQPIDLLGFLLLGRVAHKLAYYLVAILPFALVFYLCR
                     DYFPGWPSLTSFGAGVLSLILAFLLGYYLEACMGLVGFWWLEVSSLLFVYMLFNFFLS
                     GHMFPLDMLPEPWHTVVALTPLQYLAYFPAAVLLGKIQGWDLARGLATEAGWVVLFVV
                     LSRVMLKFGTRRYSAYGG"
BASE COUNT          816 a         1146 c         1067 g          622 t
ORIGIN      
        1 ctgccggctg tccaggcggc gcgcgaagca agccgccgct cacaatgcaa gaacaacctc
       61 aagcagattg ggttggcgct gcaaaactac cacgatatcc tcgggagttt tccggccggc
      121 tggatcgata cgaacaatac ggccacctgg gaatgttggg gctggggagc cttgctcctg
      181 ccctatatgg aacaaaaagg attgcatgaa agtctgggcg tggatcgggc caatctgaag
      241 aacattctgg aaacgaatgg tcctgccgcc aaactgctgg tcgagaggcc cttgcaagca
      301 ttcatgtgtc ccacggattc gggcttcgcg gggcgaggtc aggtccatca gaatcgccat
      361 ttcgacgacg gtgcgggaac cgttgctggc ggcatcgccc cgctgcgccc ggctgtgagc
      421 aattacgtgg gcgtgatggg tcatcggcgc gtggcgacag tgaccgagaa cactggcatg
      481 ttctatggca atagctacat ccggttttct gacattctgg atggtacggc ccacacgatg
      541 gctgtcggcg agcgcgatac acggcgctgc cgctccggag catgggtcgg agtgcgcaat
      601 tcggccacgg gcacgggatc gcgcggcgta cacgtagtgg gcggccgcaa tgagccgaaa
      661 ttgaaccagc ccgatccgcc gattgcctgg gatgcgaaca acggctgtgg cgaaggcttc
      721 agcagcttcc acgccaaagg ggttcagttc gtcgcctgcg acgggtccgt ccggttcctg
      781 aacgaagaca tcaatcattt ctgggtgggc aacggtgcca acgcgcacac cgacccgctg
      841 aatggcgtgt atcagcgact cctcacacgc aacgacaacc agccgacgcc cggctacgag
      901 caatagcagc ccgttgcgac acctggcagg gaacgcctcg gcgggtcgcc tcaatcctat
      961 ccgtgttgcg gagcagacgt ctgcgtccag tttgattgtc gagtcttcgc cgagcggatc
     1021 tcatcatgcc cagtagttcg tacaaacgaa ggcttgccct ggccctcatg ctcgtcctct
     1081 tggccgcaag ttcgtccggg tgcggcggct cggccaacgt cgctcctgtg aaaggacggg
     1141 tgacgctgga cggaagtccg ctcgaaaacg cgctggtgca attctcgcct ggcggggtgg
     1201 gcgctccgtc cagcggacga accaacgccc agggcgaata cgagcttact tacaccgagg
     1261 acgtgaaagg ggccgtcatc ggcaaccatg tcgttcgcgt gacgacgtat caaagcggcg
     1321 accccgacgc cgatcccccg cggccggaag ttgccgaaag ggttcctgaa aagtacgccg
     1381 cgggaatccc gaaggaggtc aaacccggcg ccaacgtcat taatattgaa ctgtagttct
     1441 ctgcaccccc gcatcgccca gggaaacgcg actatcgcca cgcgctgtcg ctctcttcgt
     1501 gcggctggcg ggcggtcctc agcaagtcca agagcgatct caattcgccc gcggaaaggt
     1561 gcccaagctg ccgctggtgg caatgccgaa ccggctcttc caaccgccgg aggagttcca
     1621 gtcccgccga cgtgatcgca atccgcacga cgcggcggtt gtccgccatt cgctggcggg
     1681 caacgagtcc gcgctgctcc agccgatcga ccagccgggt gatgtccggg gctcgagaca
     1741 ccaggcgtcc ggccaattcc agcgtggcca gtgagcccgg atgctccgcc ttcaaaagcc
     1801 gcagcgcgtt gtactgttgt gccgtcaggt cgtgctcgga aaagagcgcg tcctccagca
     1861 tccgcagtcg gtcgtaggtc cgccacaagc tcaaaaaggc ttcctgctgt ggagaatcga
     1921 agcggcgggt gtgctttcgc ggggcgttgg cggtggacat tccacgatat tacttggaac
     1981 aacaagcgtt ggccagacaa ccgtcgcgcc gctcgggccc ctcttccagc ctgtcaagaa
     2041 tcgcctctgc cgctcgctac gacgacgcgc tgcgatagct ccgcagcgcc gcctgaaaga
     2101 tccagcgcga taccaccagg ctgcccgccg tcgccagcag cgtgaaggcc gccagcggcc
     2161 agacggattc ccccgtcgga gcaatcggcc ggacgagaat ccgcgcgggc acgttcacga
     2221 ccaccagcac cggaatgatg aatgtgcaag tccactgcaa cggcgtgccg aatcgaccgc
     2281 tgtagatttc catcggatag cggctgaagt tggtgatata gaaccagaag tcgtacagtg
     2341 agcgattctg tccgagccaa atgctcgtgg cagacagcgc gatcatcagg ctgtatagaa
     2401 tcgccacccc acacagcaca tagaagatat acagcacgac catctccgac cgcagcgcca
     2461 gcggctgggt gtcgcgcgtc gtcaattgca ccaggctgac caccagcagc accagccccg
     2521 cggcaaaatt tgccaggctc gaccaatcga ccttctcgag cgagatcagg aactgcgtat
     2581 cgatcggctt gagcagggcg aaatcgagac cgccggtgcg gatcaaatcg ctcatttcct
     2641 cgcagttcgg catgaagaac gcctgcacga tgctgttgac gagcatcgtt gtcgccagga
     2701 aggcaaaaaa ttcccattgt ccccagcccg tacccgcgcc gatcgaccgg gtgtactgaa
     2761 aaatcaacaa ataaaaaccc aggttcatca tcgtccaggt gacgctgctc acgcattcga
     2821 cgatgaaatt tgtgcgaaac atcatgttcc gcaccaggct gttccgggcg aacgtggcga
     2881 atacacggag atagtcggca cgcgatgtca tattcgacat tgactcacaa ctcaccactc
     2941 acccgccgta ggcgctatac cgccgagtcc caaacttcag catcacccgc gacagcacga
     3001 cgaacagcac cacccagccc gcttcggtcg ccaaaccgcg cgcgaggtcc caaccttgaa
     3061 tcttgccgag gagcaccgcg gcaggaaaat aggccaaata ctgcagaggc gtcagtgcaa
     3121 ccaccgtgtg ccagggctcc ggcaacatgt cgagcgggaa catgtgtccg gagaggaaaa
     3181 agttgaacag catgtagaca aacaaaagcg agctcacttc cagccaccag aagccgacca
     3241 gccccatgca cgcttccagg tagtagccga gcaagaatgc gagaatcagc gacagcactc
     3301 ccgcgccaaa cgacgtcagg ctcggccagc cagggaaata atcgcgacag agatagaaca
     3361 ccagcgcaaa cggcaggatc gcgaccaggt aataggccag cttgtgggcg actcgcccca
     3421 ggagcaaaaa tcccagcagg tcgatcggct ggatcaggaa cttcttgatc tctccgctgc
     3481 gaatctgcgc cgcaatgccc gaggccaggc ccggcatgct cgagaacgcg cgggccacca
     3541 tcgtcagcaa ataatacgcc acaaagtctt cgtacttgta accggccagc gcttcgcctt
     3601 ttcgtgcttc aaacaccgca taccacagga atacctgcgt cacgatcggc a
//
LOCUS       DASUGB010000577         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1037971, whole genome shotgun sequence.
ACCESSION   DASUGB010000577 DASUGB010000000
VERSION     DASUGB010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037971"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="rpoB"
                     /locus_tag="VFV87_11040"
     CDS             complement(<1..684)
                     /gene="rpoB"
                     /locus_tag="VFV87_11040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095068.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5104339.1"
                     /translation="MAIPAQRRLTPSETRRFGSGREAHFIPDLTQIQTESYAAFLQEE
                     TAPDKRKDHGLESVLREIFPIESYDKQIKLEYLYYELGKPRYTPEECRQLRLTYGRPF
                     RIWLRLNKEQPIEEEVYLGDIPIMLGGGEFIINGAERVVVSQLHRSPGVDFVMDQEGT
                     SDRKLPSCRIIPERGSWIEVNVTKKEALSIRIDQSGKFSALTLLRAMSPKYSSDTDII
                     RAFYETTTEK"
     gene            complement(1002..1394)
                     /gene="rplL"
                     /locus_tag="VFV87_11045"
     CDS             complement(1002..1394)
                     /gene="rplL"
                     /locus_tag="VFV87_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5104340.1"
                     /translation="MAETVDITSLGDQIANLTLKQAKELSDYIKEKYGIEPAAGGAVM
                     MAAPADGGGGAAAAAEQTEFDVVLTSFGDKKLDVVKVVKNLTGASLMDAKKLVEGVPA
                     KIKEKVSKEDAEKVKAELTAVGATVELK"
     gene            complement(1499..2032)
                     /gene="rplJ"
                     /locus_tag="VFV87_11050"
     CDS             complement(1499..2032)
                     /gene="rplJ"
                     /locus_tag="VFV87_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5104341.1"
                     /translation="MSKQLKELISTELRKRLEGVSDALLVNVIGLNSENTFALRKELR
                     SKDIHLLVVKNSMAKRATEGTPLAKAFDDCEGSLAVVWGAEDFVSLAKELVAIHKKPE
                     FEKCQARGGVMEGEQLTAEKVKEISKWPNRAGQISILLGQILSPGSKLLSQINAPGGK
                     LLSQVKQKSEGEEAPTA"
     gene            complement(2128..2808)
                     /gene="rplA"
                     /locus_tag="VFV87_11055"
     CDS             complement(2128..2808)
                     /gene="rplA"
                     /locus_tag="VFV87_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5104342.1"
                     /translation="MAKRSKRYKAIAGESDIKNPIPLATAVEKLKKYGTTKFDQTVEI
                     HMRLGVDPKQADQIIRGSVVLPHGIGKTQRVAVFAKGDLAEAAKSAGADVVGQEDLAQ
                     KIKDGWTDFDVCIAAPDMMGLVGPLGKVLGPRGLMPSPRAGTVTPDVAKVVKEYKAGK
                     VEFRNDDGGIVHAVVGKLSFDATMLRENTETLINFVLGLKPNSVKGQYVKSVAICGTM
                     SPSIYVAV"
     gene            complement(2816..3247)
                     /gene="rplK"
                     /locus_tag="VFV87_11060"
     CDS             complement(2816..3247)
                     /gene="rplK"
                     /locus_tag="VFV87_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5104343.1"
                     /translation="MAKAKQLTGQAKFQVPGGSATPAPPVGTSLGKFGINLGQFVSQF
                     NERTRQFNGIPIPVIVSVFSDRSFEFKCKSPPASALLKIKAGIAKGSGVPNKDKVGKV
                     TRAQCVEIVKEKWEDLNARDHEHAVSMVEGTARSMGIIVEG"
     gene            complement(3369..>4224)
                     /gene="nusG"
                     /locus_tag="VFV87_11065"
     CDS             complement(3369..>4224)
                     /gene="nusG"
                     /locus_tag="VFV87_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5104344.1"
                     /translation="PAGEPAEGPPVLPPDQPVEQSPDSLQPPAEDSAPASSGTPTEPM
                     AAWSNAQQPAAESPAAEESAEAEAEADELDEPDEPDEPEPDVPFAAPLEFIDETIAEE
                     DLKFDWFILKVQVNREDSIKDALLRRVKMNGLDRYFKEIVVPTEDVVEFTKTGKKRVV
                     KKKLYPGYILVNMSVSDESWFMVRETPGIGDFTGASGKPTPMDPKDVERILRSSKVLP
                     DEEGEPEKIGIPFKDGDRVRVKEGYFQNFEGDVDKIDQATGRVTVMVNIFGRSTPVEL
                     DHWQIEAV"
BASE COUNT          686 a         1290 c         1296 g          952 t
ORIGIN      
        1 cttctcggtt gtcgtttcgt aaaacgcccg gatgatgtcg gtgtcgctgc tgtatttggg
       61 gctcatcgcc cgcaagagcg tcagggccga aaacttgccg ctctggtcaa tgcggatgct
      121 cagcgcctcc ttcttcgtga cgttcacctc gatccagctt ccacgctccg gaatgatgcg
      181 gcagcttggc agcttgcggt cgctcgtccc ttcctggtcc atcacaaaat cgacgcccgg
      241 gctgcgatgg agctggctga cgaccacgcg ttccgccccg ttgatgatga actcgccgcc
      301 gccgagcatg atcggaatgt cgcccagata gacctcttcc tcgatcggct gctccttgtt
      361 cagccgcagc cagatgcgga acggccggcc gtaggtcagg cgaagctggc ggcactcctc
      421 gggcgtatag cgcggcttgc cgagttcgta gtagaggtat tcgagcttaa tctgcttgtc
      481 gtagctttcg atcggaaaga tctcgcgcag gaccgactcc aggccgtgat ccttgcgctt
      541 gtcgggcgcg gtctcttctt gcaggaaggc ggcgtagctt tcggtctgaa tctgagtcag
      601 gtcgggaatg aagtgggctt cgcgtccgct gccgaaacgc cgggtttcgc tgggagtcaa
      661 gcgtcgctgt gcggggatgg ccataagcgc cagttctccg agaggggtga tcgggctggt
      721 gagtcagcgt cttccttgac ggaacctagg aacaacaacg tggccagcga gcggccgggc
      781 ggggagccaa gggcaccgtg ccaatacatg aacaaatgtc tgcgccttcg gcgcgcagct
      841 tcaccccatg ccaatccagt tgcacaaaac tgctggaaag cagtggggtg gaacgcaatt
      901 cagtctttcc tgcccgcacg cgctgccgcg gggcgcacgg caacttgtgc gttggtgagg
      961 tcgtcacttc ctgtaagctt aacgaatctg cgccgttttg gctacttaag ttcgaccgtg
     1021 gcgccaactg ccgtcagttc cgccttcact ttctcggcat cttccttgga cactttttcc
     1081 ttgatcttgg cggggacgcc ttcgaccagc ttcttggcgt ccatcagcga agcgccggtg
     1141 aggttcttca caaccttcac gacgtccaac ttcttgtcgc cgaacgaagt gaggacgacg
     1201 tcgaactccg tctgctcggc agcagccgcg gcgccgccgc cgccatccgc cggggcggcc
     1261 atcattaccg cgcccccggc agcgggctcg atgccatact tttccttgat gtagtcgctc
     1321 agttcctttg cctgcttgag cgtcaggttg gcgatttgat cacccaggga ggtgatatcg
     1381 acagtctcgg ccatgggggg tcctttcgtg ggggattttg gattttggat tgccgatttt
     1441 ggattccttg ggactttggc gctcgaatcc aaaatccaaa atccaaaatc taaaactgct
     1501 aagcggtcgg cgcttcctcg ccctccgact tctgtttgac ctggctgagc agcttgccgc
     1561 cgggggcgtt gatctgcgag agcagcttgc tgccgggcga gaggatctgc cctaagagga
     1621 tgctgatctg gccggcgcgg ttcggccact tgctgatttc cttgaccttc tcggcggtga
     1681 gctgttcacc ctccatgacg ccgccgcggg cctggcactt ctcaaactcg ggctttttgt
     1741 ggattgcgac cagctccttg gcgagcgaga cgaaatcctc cgcgccccag acgaccgcca
     1801 ggctcccctc gcagtcgtcg aatgctttgg ccagcggcgt cccttcggtc gcccgcttgg
     1861 ccatgctgtt cttaacgacc agcaagtgga tgtccttgct ccgcaattcc ttccgcaggg
     1921 cgaacgtgtt ttccgaattg agcccgatca cgttcacgag cagcgcgtcg ctcacgcctt
     1981 ccaggcgctt gcgaagctcg gtgctgatca gctctttgag ttgtttgctc atggcagtta
     2041 gcgctgtagg gaacgccctc cgcggcgttc cgctgtcggt acgttgcgcg aatcacggaa
     2101 cggcacggag gccgttccct acaggtgtta gacggctaca taaatcgaag ggctcatcgt
     2161 gccgcagatg gcgacgctct tgacgtactg tcccttgacc gagttcggct tcaggcccag
     2221 gacgaagttg atcaacgttt cggtgttctc gcggagcatg gtggcgtcga agctcagctt
     2281 accgacgacg gcgtggacga tcccgccgtc gtcgttgcgg aactccactt tgcccgcctt
     2341 atattctttg acgaccttgg cgacgtcggg cgtgacggtg ccggcgcgag gcgagggcat
     2401 caggccgcgt gggccaagca ccttccccag cgggccgacg aggcccatca tatctggggc
     2461 ggcgatgcac acgtcgaaat ccgtccagcc gtctttgatc ttctgagcaa ggtcttcctg
     2521 accgacgaca tcggccccgg cggatttggc ggcttcggcc aggtcgccct tggcgaagac
     2581 ggccacgcgc tgcgtcttgc cgattccgtg cggcagcacg accgagccgc ggatgatctg
     2641 atcggcctgc ttgggatcga cccccagccg catgtggatc tcgaccgtct ggtcgaactt
     2701 cgtagtgccg tatttcttca gcttttcgac ggcggtggcc agcggaatcg gattcttgat
     2761 gtccgattcg ccggcgatcg ccttgtagcg tttggagcgt ttggccatgg cggctttacc
     2821 cttcgacgat gatccccatg ctgcgggcgg ttccctcgac catgctcacg gcatgctcgt
     2881 ggtcgcgggc gttcaggtct tcccactttt ccttgacgat ctcgacgcac tgggcgcggg
     2941 tgaccttgcc caccttgtcc ttgttaggca cgccggagcc cttggcgatg cccgccttga
     3001 tcttcaacaa agccgaagcg ggggggctct tgcacttgaa ttcgaacgag cggtcactaa
     3061 agaccgagac gatcaccggg atcggaatgc cgttgaactg ccgcgtccgc tcgttgaact
     3121 gcgagacgaa ctggcccagg ttgatgccga atttgcccag cgacgtgcca accggggggg
     3181 ccggagtggc cgagccgccg ggtacctgga acttggcctg gccggtgagt tgttttgctt
     3241 ttgccatgtg tcaaacgtat ccgtcaagag gcgaattgcg aatgggggtg gtagccgcgt
     3301 tcgccagaac gcgggcttat ttgactgaca ctcacccgca ctctggcgag tgcggctacc
     3361 agacctttct aaaccgcctc aatttgccag tgatcgagct ccacgggcgt gctgcggccg
     3421 aagatgttga ccatgaccgt cacgcggccg gtggcctggt cgatcttgtc cacgtcgcct
     3481 tcgaaattct ggaaatagcc ttccttgacg cggacgcggt caccgtcctt gaacggaatg
     3541 ccgatcttct cgggctcgcc ttcctcgtcc ggcaggactt tgctggaacg gagaatccgc
     3601 tcgacgtcct tggggtccat cggagtcggt ttgccgctgg cgccggtgaa atcgccgatg
     3661 ccgggcgttt cgcggaccat gaaccacgat tcgtcgctca ccgacatgtt caccaggata
     3721 tagcccgggt agagcttctt cttgaccacc cgctttttgc cggtcttggt gaattcgact
     3781 acgtcctcgg tggggacgac gatctctttg aaatagcgat cgagcccgtt cattttgacc
     3841 cgccgcaaga gcgcgtcttt gatcgaatcc tcgcggttca cctggacctt gaggatgaac
     3901 cagtcgaact tcagatcctc ttcagcgatc gtctcgtcga tgaattccag cggggcggcg
     3961 aaggggacat ccggctcagg ctcatccggc tcgtcgggtt cgtcgagttc gtcggcttcg
     4021 gcctccgctt ccgcggactc ttcagcagcc ggggactcgg cagccggctg ttgggcattc
     4081 gaccaggcgg ccatgggttc ggtgggggtt cccgaggagg cgggggccga atcttccgcc
     4141 ggcggctgca gagaatccgg gctctgttcg accggctgat ccggcggcag gacgggcgga
     4201 ccctcagccg gctctccagc aggc
//
LOCUS       DASUGB010000578         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1176634, whole genome shotgun sequence.
ACCESSION   DASUGB010000578 DASUGB010000000
VERSION     DASUGB010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176634"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFV87_11070"
     CDS             complement(<1..426)
                     /locus_tag="VFV87_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104345.1"
                     /translation="MMDALYEIADIPAGDRLAMHVLVIEDDPAIGKALERGLNEAGHP
                     SDLERSGTKGLEKALTQRYDAIVLDLMLPDLAGLELMRRLRASGVRTPVLMLTALGAV
                     EDRVTGLTSGADDYLVKPFAFAELLARLEAICRRTSDRPS"
     gene            806..>3319
                     /locus_tag="VFV87_11075"
     CDS             806..>3319
                     /locus_tag="VFV87_11075"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEX5104346.1"
                     /translation="MDGSNRATATLETASDDEAPPLPAPVPDRHERLAHVIEHAAHYL
                     PAQGPITVFIHHNTLHAFEELPFDAGVQKGAALFGCQPYLSEDRYRDLLRRSRIRSAD
                     LVAELLDDLDERADELLGFMGTRFNLRLAMLQYPLKTAPAAELRWFVAETDALTRFRA
                     DAPADVRQKFLEQTRRWVMRDLRNSVAPAESGKSADQLLHLRQALASLIDHYGRSSIE
                     EWSDATWEAFTLQTLWRVCREGVHGLKLPAPSQPAPLRHRDLLLDATGADSDELVHEV
                     LIGFCAAFVDQGFSRWPLPDREQGFFQAFSAVYRQAGSPLEGWLRPLGAELERLAAAN
                     VQPLDSIHESLELLGVPPAEWESFVTSTYLALRGWGGMVRQVELRADSVAHAIPQSSL
                     TEMLAVRLILDRLAVAWVAQQSLEFSGPLSEVRSAAREARSPHEMDGLDQRAFKIFQL
                     AQVLGWLPLDLARLSKMDWGRLTAEIEAFPPLEMRRVFHAAFERQYRVQSLDAIAVQS
                     QQPTGRVANPRFQVFCCLDEREESFRRHLEEIAPDVETFGAAGFYGVPMYYRGVGDAH
                     FVPLCPIVIRPQHWVCEGVNDHHDHLNTRRAKARRAVGLASHQMHLGSRGFVGGAVLG
                     VMGALASIPLVARVMFPRLTARLTQMFGSFVEPPSQTRLQLERSEEKPGIEPGNVGYS
                     LDEMANMSERLLRDLGLTSGFARLVIVIGHGSYSVNNPHISAYNCGACGGSCGGPNAR
                     ALAEILNDPRVREKLKSRGLVIPDETVFIGSWHNTCNDGMTYFDLERIPPAHREEFDR
                     ARLELEEACDRNAHERCRRFVSAPLNMSFAAARRHVENRA"
BASE COUNT          562 a         1049 c         1063 g          645 t
ORIGIN      
        1 cgaaggccgg tcgctggtcc gccgacagat ggcctccagc cgcgccagca gctcggcaaa
       61 ggcaaaaggc ttcaccagat agtcgtcggc gcccgacgtc aatcccgtca cgcggtcttc
      121 gaccgcgccc aatgccgtta gcatcagcac cggagtccgc acgccgctgg ctcgcaggcg
      181 ccgcatcagc tcgagccccg ccaggtcggg gagcatcagg tcgaggacga tggcgtcgta
      241 ccgctgcgtg agggccttct ccagcccttt cgttccgctg cgttccagat cgctcggatg
      301 tccggcttcg ttcaagccac gctccagcgc tttgccgatg gccggatcgt cttcgatcac
      361 aagcacgtgc atcgcgaggc gatctcctgc cgggatatcg gcaatttcat aaagagcgtc
      421 catcatgatc ccacgtgtga gagaaatcga gagccttcga tgtggcaacg ctgtgaccgg
      481 gcagcgaacc gatttcctcc atcgtgcggt agttcgacct tcgtgaacag tgcggattgc
      541 tgattcgtaa aacaggaagt cagggagttg aggcaaccgg cattacgaaa ttgtaacaca
      601 aggtgcgtct tctcgctgat tggcccggtg ttagagtgac agattaatct ctcccgccgc
      661 cggcctgacg aaccgaaggc gcatgcccac cagcgtcgct ccatgcccac ggcggcatgg
      721 tctcgagcaa cggacggttt gagtcgcgac cccgcgtgca ggacgcaatc gacgaacacc
      781 atgacgcccg ctcctgggaa tctacatgga cggttctaac cgcgctacgg ccacgcttga
      841 aacggcctct gacgacgaag cgccgccgct cccggctcct gtgcctgacc gccatgagcg
      901 cctggctcac gtgatcgaac acgcggccca ttatctgccc gcccaaggac cgatcacggt
      961 cttcattcac cacaacacgc tgcacgcctt tgaggaactg cctttcgatg ccggcgtcca
     1021 gaaaggcgcc gcgctgttcg ggtgtcagcc ctatttgagc gaggatcgct accgcgatct
     1081 gctccggcgg tcgcggattc gcagcgcgga tctggtcgcc gagctgctcg atgacctgga
     1141 cgagcgggcc gatgagctgt tgggctttat gggcacgcgg ttcaacctgc ggctggccat
     1201 gctgcagtac ccgttgaaga ccgcgccggc agcggaactt cgctggtttg tggcagaaac
     1261 cgatgccctg acccgctttc gcgccgatgc gcctgccgac gtgcgccaga agtttctcga
     1321 gcaaactcgc cgctgggtca tgcgcgacct gcgcaatagc gttgctccgg ccgagtcagg
     1381 caagtcggca gaccagcttc tgcacttgcg acaggctctg gccagcctca tcgatcacta
     1441 cggccgtagc tcgatcgagg agtggagcga cgccacgtgg gaagccttca ccttgcagac
     1501 attgtggcgc gtctgccgtg aaggggtcca tggactgaag ctgccggcgc cgtctcagcc
     1561 cgcgccgctg cggcatcgcg atttgctgct cgatgccacc ggcgccgaca gcgacgagct
     1621 cgtacacgaa gtgctgatcg ggttttgcgc cgccttcgtc gatcagggtt tttcccgctg
     1681 gcctttgccc gaccgcgagc aggggttctt tcaggcgttc agcgcggtct atcggcaggc
     1741 cggcagcccg ctcgaaggct ggctgcgccc cttgggcgcc gagctcgagc gtttggcggc
     1801 tgccaacgtg cagccgctgg attcgatcca cgaatcgctg gagctgctgg gtgttccgcc
     1861 cgccgagtgg gagtcgttcg ttaccagtac ttatctggcc ctccgcggct ggggcggcat
     1921 ggtgcgccag gtggagttgc gagccgacag cgtggcgcat gcgattcccc agagcagtct
     1981 caccgaaatg ctggccgtgc ggcttattct ggaccggctg gcggtggcct gggtcgcgca
     2041 gcagtcgttg gagttttccg gtccgctctc cgaggtccgc tccgcagcca gggaggcccg
     2101 ctcgccgcac gaaatggacg ggctcgatca gcgggccttc aaaatctttc aactcgcaca
     2161 ggtgctgggc tggctgcctc tggatctggc gcggctttcc aaaatggatt ggggacggct
     2221 gacggccgaa atcgaggcgt ttcctccgct ggaaatgcgg cgcgtgtttc acgcggcttt
     2281 cgagcggcaa taccgcgtgc aatcgctcga cgcgattgcg gtccaatccc agcagccgac
     2341 cggtcgcgtg gccaaccctc gatttcaagt gttttgctgt ctggatgagc gcgaggagtc
     2401 gtttcggcgt catctggaag aaatcgctcc cgatgtggag acgtttgggg ccgccggttt
     2461 ttacggcgtg ccgatgtatt atcgcggtgt gggcgacgct cactttgtgc cgctctgccc
     2521 catcgtcatt cgccctcagc actgggtttg cgagggcgtc aacgatcatc acgatcatct
     2581 caacacgcgc cgagcgaagg cccgccgtgc tgtgggcctc gcctcgcacc agatgcacct
     2641 cggcagccgg ggcttcgtcg gcggcgccgt tctgggcgtg atgggcgcgt tggcgtcgat
     2701 cccgctggtg gcccgggtca tgttccccag gctcaccgcc cgcttgactc agatgttcgg
     2761 gagctttgta gagccgcctt cacagacgcg gctgcagttg gaaaggtccg aggagaagcc
     2821 gggaatcgag ccggggaacg ttggctattc actggatgaa atggccaaca tgtcggaacg
     2881 gctcttgcgc gatctgggat tgaccagcgg tttcgcccga ttggtgatcg tcatcgggca
     2941 cggctcgtac agtgtgaaca acccgcacat ttcggcttac aactgcggcg cctgcggcgg
     3001 cagttgcggc gggccgaacg ccagggcgtt ggccgagatt ctcaacgacc cgcgcgtgcg
     3061 cgagaagctg aaatcccgcg gcctggtgat ccccgacgag acggttttca tcggaagctg
     3121 gcacaacacg tgtaatgacg gcatgacgta tttcgacctg gagcggattc ccccggcgca
     3181 ccgggaggag ttcgaccggg cgcgattgga attggaagaa gcgtgcgacc gcaatgccca
     3241 cgaacgctgc cgccgattcg tttcggcacc gctcaacatg tcgtttgccg ccgcgcggcg
     3301 gcacgtggag aaccgggcc
//
LOCUS       DASUGB010000579         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_620392, whole genome shotgun sequence.
ACCESSION   DASUGB010000579 DASUGB010000000
VERSION     DASUGB010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620392"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFV87_11080"
     CDS             <1..876
                     /locus_tag="VFV87_11080"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX5104347.1"
                     /translation="GITKWDFGPLPEHVDLNSSGLTLTKYPAVVDAGQSVNLRLVDSA
                     VEAGRLSRMGVLRLFAIAEHRELKAQIKHLPQIERIRLYAAPLSKHQPIDEQIIDLLA
                     SRAFYATDNVPHNDTSFEAQRLLGRRNILPTVQEITKLVLPLFGAYHEVRLALEQPRP
                     AAGEYALADIRQQLETLLSEGFLVRTPWTWLQHVPRYLRAIGVRLGKLVGSLPRDRQS
                     HAEIAPREAALAACALRQHWANLADPELAHFRWMLEELRVSSFAQDLGTSIPVSTQRL
                     DRQWKNSESRGKIMP"
     gene            1116..2108
                     /locus_tag="VFV87_11085"
     CDS             1116..2108
                     /locus_tag="VFV87_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5104348.1"
                     /translation="MPRPVTLFTGQWADLPLEQMARKTKEFGYDGIELACWGDHFEVE
                     KADDAYCRRKRDLLDSHGLQCHAISAHLVGQAVCDIIDERHKSILPPAVWGDGKPEGV
                     NKRAAEELMNTARAAKRFGVGVVNGFTGSSIWPLLYSFPPVPGKMIDDGFALFANRFN
                     PIFDVFAECGVKFALEVHPTEIAFDIYTAERALEAIGRRPEFGFNFDPSHLHWQGIDS
                     VEFLRTFSDRIYHVHVKDAIVTLNGKSGILASHMNFGDHRRGWDFRSPGRGTVNFEEI
                     IRELNRIGYQGPLSVEWEDSGMDREFGAREAAGFVKQKDFAASGRAFDAAFEKA"
     gene            2296..2508
                     /locus_tag="VFV87_11090"
     CDS             2296..2508
                     /locus_tag="VFV87_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104349.1"
                     /translation="MWKIIKLGIATLAALYTLGVVIGFVSNLGQHGGTRGVTHLAAGI
                     GVTAASAAITWWLFTWALKKPNPPRV"
     gene            2802..3167
                     /locus_tag="VFV87_11095"
     CDS             2802..3167
                     /locus_tag="VFV87_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104350.1"
                     /translation="MNKPAKADSIQRWPARLLVEFKAMERDIDRIIAAVKARLPTVEV
                     QQFKATHPADDDGVWWFSLPGVNADVQIDSRYGQCPFLVDHSDTAIPLEAVEVSTVIE
                     VAERIVTFLEAKQRAAIGT"
     gene            3256..3615
                     /locus_tag="VFV87_11100"
     CDS             3256..3615
                     /locus_tag="VFV87_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104351.1"
                     /translation="MDETTFAHLRPKERTRRYAARVQQFLEHCPQVGGYEWSMVATSC
                     KSIDFGDGLRWKWQTDNAQYRMQTKLRRFAVRNIVRYLTELDMYGNSELEAARDQLVG
                     DGKELIGIYDAIIAACT"
     gene            complement(4062..>4266)
                     /locus_tag="VFV87_11105"
     CDS             complement(4062..>4266)
                     /locus_tag="VFV87_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104352.1"
                     /translation="TSAADFATRTTNLTTGLAGGIALLLGQTIHDDGAADCLNGGTDP
                     DWLFLLAEDCQLYITLDYLVAGP"
BASE COUNT          814 a         1356 c         1334 g          762 t
ORIGIN      
        1 ggcattacca aatgggattt cggcccgctg cccgaacacg tcgatctgaa ttccagcggc
       61 ttgacgctca cgaagtaccc agccgtcgtc gatgccggcc aatcggtcaa cttgcgactc
      121 gtcgattccg ccgtcgaagc cggccgactc tcgcggatgg gcgtcctgcg gctctttgca
      181 attgccgagc atcgcgagct caaggcccag ataaagcatc tgccgcagat cgagcgaatc
      241 cgactttacg ccgcgccgtt aagcaagcac cagccgatcg atgagcaaat catcgatctg
      301 ctcgcctccc gcgcgttcta cgcgaccgac aacgttccgc acaacgacac gtcatttgag
      361 gcccagcgcc tcctcggccg ccggaacatc ctcccgaccg tgcaggaaat caccaagctc
      421 gtcctaccgc tcttcggggc ctatcacgag gtccgactgg ctctcgaaca gccgcgaccg
      481 gctgccggcg agtacgcgct ggccgatatt cgccaacagc ttgagacgct cctttccgag
      541 ggattcctcg ttcgcactcc ctggacctgg ctgcagcacg tgccgcggta tctgcgagcg
      601 atcggagtcc gcctcggcaa gctggtggga agcctgcccc gcgatcgcca gtcccacgcg
      661 gaaatcgcac cgcgggaagc agccctcgcg gcatgcgccc ttcgccagca ttgggcgaat
      721 ctcgccgatc cagagctcgc gcacttccgc tggatgctcg aagagttgcg agtttcctca
      781 ttcgcccagg atctgggcac gagcattccc gtctcgaccc agcggctgga caggcaatgg
      841 aagaattccg aatcgcgggg aaaaatcatg ccctgacaat cggctcgaat tcctcaagtg
      901 tgccgtggaa accaggcttc cgcttcgtca caaccagacc ggcgattctc atgcgccgac
      961 tgacgcacgg aaggacggac cgttcgccgc ggcggttgca ggtcgctgat ttcgtcgcca
     1021 caatgacggc tctcgccgcg cgggcctctc cgcttctggc gacgaacttt ctcgccattc
     1081 ccgcacctga aatcgccata gaaagggttc tctccatgcc tcgccccgtg acgctcttca
     1141 ccggccaatg ggccgatttg cccctggagc agatggcccg caaaaccaag gaattcggct
     1201 acgacggcat cgagctggcg tgctgggggg accattttga ggtcgagaag gccgacgatg
     1261 cctattgccg ccgcaagcgc gacctgctcg acagccacgg cctgcagtgc cacgcgatca
     1321 gcgcccacct cgtcggccag gcggtctgcg acatcatcga cgagcggcac aagagcatcc
     1381 tcccacccgc ggtgtggggg gacggcaagc cggaaggcgt caacaagcgg gccgccgagg
     1441 agctgatgaa caccgcccgc gccgccaagc ggttcggcgt cggcgtggtc aacggcttca
     1501 cgggcagctc gatctggccc ctgctctaca gcttcccgcc ggtgccgggc aagatgatcg
     1561 acgacggctt tgcgctgttc gccaaccggt tcaatccgat tttcgacgtc tttgccgaat
     1621 gcggcgtcaa attcgccctc gaagtccacc cgaccgagat cgccttcgac atctacaccg
     1681 ccgagcgggc cctggaagcg atcggccgcc ggccggagtt cggcttcaat ttcgatccga
     1741 gccacctgca ctggcagggg atcgattcgg tcgagttcct gcggaccttc agcgaccgca
     1801 tctatcatgt gcacgtcaag gacgcgatcg tcacgctcaa cggcaagagc ggcatcctcg
     1861 ccagccacat gaacttcggc gaccaccgcc gcggatggga cttccgcagc cccggccgcg
     1921 gcacggtcaa cttcgaggag atcatccgcg agctgaaccg catcggctac cagggcccgc
     1981 tctccgtcga atgggaagac agcggcatgg accgcgagtt cggcgcccgc gaagccgccg
     2041 gcttcgtcaa gcaaaaagac ttcgccgcca gcggccgggc gttcgacgcg gcgttcgaga
     2101 aggcctaacg tggagtaggc gtctcgcctg cctcttctcc ctccccctct gggagagggc
     2161 cggggtgagg gtgatggcgc tgtagggaac gccctccgtg gcgttccgcg ggtgagtgca
     2221 gcggtggggc ggctcgcccg cgccccaaag ccgcgaccat tggtcgcggt tcccagcacc
     2281 ggcaggacca cattcatgtg gaaaatcatc aagctgggca tcgcgaccct cgccgcgctc
     2341 tacacgctcg gcgtcgtcat cgggttcgtc tccaacctcg gccaacacgg cggcacccgc
     2401 ggcgtcacgc atctcgccgc cggcatcggc gtcaccgccg cctccgccgc catcacctgg
     2461 tggctgttta cctgggctct gaaaaagccc aacccgcccc gcgtttaacc cgcagccgca
     2521 gcgaagcgaa cgcgcagccc gccgcctgga tgccagcgca ggtgtatctc gggttggatc
     2581 gcccctccgc ctgagccttt ggctacgggt taaacgaccg cgctccgcgc tcgccgccgc
     2641 cgcagcccgc ttcagcgggc ttaccccgtc aggccccggc ttcagccggg gcaggcgcgc
     2701 aaccaacgcc ccgatttcct tcagccggct tcagccggct tgctccgcag gggcttaacc
     2761 cgcagcccgt cgctggctcg ggctaaagca acgggcctaa catgaacaag ccggctaaag
     2821 ccgactcgat acaacgctgg ccggcccggc tcctggtaga attcaaagcc atggaacgcg
     2881 acattgaccg aatcatcgct gccgttaaag cacgactgcc aacggtcgag gtccagcaat
     2941 tcaaggcaac gcatccggcc gacgacgatg gcgtgtggtg gttctcctta ccaggcgtaa
     3001 atgccgacgt gcaaatcgac tcgcggtacg ggcagtgccc gtttctggtt gatcattcgg
     3061 atacagctat cccactggag gcagttgagg tctccaccgt catcgaagtc gccgagcgca
     3121 tcgtcacgtt tctagaagca aagcaacggg cagcaattgg aacctgactt tggctgggaa
     3181 tttggacgaa gcgccggtca agcgccgatc caatactctg gattgagaaa aaagcaacct
     3241 catacaatag cgaacatgga cgagacaaca ttcgcccatc ttcgcccgaa agagcgtacg
     3301 cgccgctacg ccgcgcgcgt tcaacagttt cttgagcact gcccgcaagt tggtggctac
     3361 gagtggtcga tggtagccac ctcatgtaag agcatcgatt ttggagacgg gcttcggtgg
     3421 aaatggcaga ccgacaacgc ccaataccgc atgcagacga agttgcgccg atttgcggtc
     3481 cgcaacatcg tgcgttacct gacggagctg gatatgtacg gcaattcgga gcttgaggca
     3541 gcaagggatc agctcgtggg ggatgggaag gaattgatcg gaatctatga cgcgataatc
     3601 gcggcttgta cttaggactc cgtgacattg gtcagcgccg tcgttctgga aaacctgtac
     3661 accacccgga agggcagttg gcagggtcga ctgagttgtg tacaattgga ttgtattggt
     3721 gcacggttgc tgtgcggcct ggtgaggtcg agctgttgta gcgcgaggcg gcgggtgtca
     3781 gatgtcgtgc ttaacttgca taaaatgcat aattgcatag gagaatgaac gacgctaagt
     3841 gcttgtgggg acagtggtta ggcgatgaat gggcggcggg aaaaaagggt tggttatgca
     3901 tgcataaggt ttcggggctc ggggttcggg gataggaagt gtgggcggta ggggggcgtg
     3961 gcggtcggga gactggccgc agcggtggca ggctggaagc ctaccccacg gcggggcgcc
     4021 ctcacatgcg cggcaggctt gaaagcctgc cccacggctg actacgggcc ggctacgagg
     4081 tagtcgaggg tgatgtagag ctggcagtct tcggcgagga ggaagagcca gtcggggtcg
     4141 gtgccgccgt tgagacagtc cgcggctccg tcgtcgtgga tggtttggcc gaggaggagg
     4201 gcgatgccgc cggccaggcc ggtggtgagg ttcgtcgtgc gggtcgcgaa gtcggcagcg
     4261 ctggtc
//
LOCUS       DASUGB010000580         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_204981, whole genome shotgun sequence.
ACCESSION   DASUGB010000580 DASUGB010000000
VERSION     DASUGB010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204981"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFV87_11110"
     CDS             <1..665
                     /locus_tag="VFV87_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5104353.1"
                     /translation="SQVAFHTLVSLSDVDQIPAFRTAALRESPLVQTQFEQPIAADAP
                     RFGFPFKAIEGEPPRLVLTGVNPGSPAERSGLKTGDVLVSVDGEPIADAVRFRLRLLA
                     AAGETSLVVEREGEEEPLEIKVTPLGNPVRVGITWRMDDGEPGTALVTRVVFGSAAQA
                     AGLQLKDRIYSVGGQPFASQQDLSRLLNETPSPVELLIERSGKLQTARLDVLDLGLPA
                     AE"
     gene            824..>2099
                     /locus_tag="VFV87_11115"
     CDS             824..>2099
                     /locus_tag="VFV87_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HEX5104354.1"
                     /translation="MALTYQFQGASARVALLACAITCIAGNSQQALGQAGVAFTQQQP
                     FVIGLIPVVGRGGAVGGVSIDAQGVVARSDVDRAQRLREARLRALAEIDSRLEAASPL
                     RKISLRDLTAAIEQRMKAGLPATDEIQNLAGLTRIQYVLVYPERGDIVLAGPAEGWQV
                     NDLGCVVGVKSGQPVLQLDDLIVALRTAKEQPKTGELITCSIDPTAEGLTRFTRMLNS
                     RDLPINDATVAGLEQAVGPQQVTLTGVPPGTHFAQVLVAADFLMKRLAMNFEPAPIDG
                     LPSYMEMLQADRGPAPRNAMPRWWMTASYEPLLKDEAGLAWQLRGPGVQTLSEDGILG
                     RGGKAVAREAKGNSLAKKWADTMTASYDALADKLPVFAELRNCMDLAVVGALLVKEDL
                     PGRAGCDLSLLLEDKRVAVAEYHVPKTIDSRAS"
BASE COUNT          359 a          692 c          696 g          352 t
ORIGIN      
        1 gctcgcaggt tgccttccac acgctcgtca gcttgtccga cgtcgatcaa atcccggcct
       61 tccgcaccgc cgcgctccgc gagtcgccgc tcgtgcagac gcagttcgag cagccaatcg
      121 ccgccgacgc gccgcgcttt ggctttccat tcaaagcgat cgaaggagaa ccgccgcgac
      181 tggtgctgac gggggtcaat cctggctcgc cggctgaacg gtcggggctt aagacggggg
      241 acgtgctcgt ttcggtcgat ggcgagccga tcgccgatgc cgtccgcttc cgcctgcggc
      301 tgctggccgc cgccggcgaa acgagtttgg tggtcgagcg cgaaggcgag gaagagccgc
      361 tcgaaatcaa ggtgacgccc ctgggcaatc ccgtccgtgt gggcattacc tggcggatgg
      421 acgacggcga acccggcacg gcccttgtca cacgcgtcgt cttcggctcc gcagcccagg
      481 cagcggggct gcagctcaag gaccgcatct attctgtcgg tggccagccc ttcgccagcc
      541 agcaggacct gtcgcgtctg ctcaacgaaa cccccagccc ggtggaactc ctcatcgaac
      601 gctccggcaa gctgcaaact gcccgactgg atgtgctcga ccttggcctg ccggccgcgg
      661 aatgagttcc atactggatt tccgacagtt ctaccgctaa tctgtccatt gcagtccagg
      721 acgtcgctcg ctgttccgct tctgattggc ctcctcgaag atcgccggct cgctccggac
      781 tgttggcagg ggcgaaggct ctatttgttc gaggaggccg gcgatggcat tgacctacca
      841 atttcaggga gcttctgcgc gcgtggcact gttggcgtgc gcaatcacgt gcattgcggg
      901 aaactcgcag caggcgctgg gccaggcggg cgtggcattc acgcagcaac agccattcgt
      961 catcgggctg attccagtcg ttggccgcgg cggcgcagtc ggcggcgtgt cgatcgatgc
     1021 ccagggggtg gtggcgcgaa gcgatgtcga tcgggcgcag cgcctccgcg aagcccggct
     1081 gcgggcgctg gccgagatcg atagtcgctt ggaagccgcc agcccgctga ggaagatttc
     1141 gctccgcgac ctgacggcgg cgatcgagca gcggatgaag gccggcctgc cggcgaccga
     1201 cgagattcag aacctcgcgg gcctgacgcg gattcagtac gtcctcgtgt atcccgagcg
     1261 gggcgacatc gtcctggccg gcccggccga aggctggcaa gtcaacgacc tgggctgtgt
     1321 cgtcggagtc aagagcggcc aacccgtgct gcagcttgat gacttgatcg tcgcgctccg
     1381 caccgcaaaa gagcagccga aaaccggcga attgatcacc tgctcgattg acccgaccgc
     1441 ggaaggcctc acgcgtttca cgcggatgct caacagccgc gatctgccga tcaacgacgc
     1501 tacggtcgcc gggctcgagc aagcggttgg cccccagcag gtgacgctca cgggcgtgcc
     1561 gccgggaacg cacttcgccc aggtgcttgt cgcggctgat ttcctgatga agcgcctggc
     1621 gatgaacttt gagccggcgc cgatcgacgg cctgccgagc tacatggaaa tgctccaggc
     1681 cgatcgcggg cctgcgccca ggaacgccat gccccgctgg tggatgacgg ccagctacga
     1741 gccgctgttg aaggacgaag cgggcctcgc ctggcagctt cgcggcccag gcgtgcaaac
     1801 gctgagcgag gatggcatcc tcggccgcgg aggcaaggcc gtcgcccgcg aggcgaaagg
     1861 caactcgctc gcgaagaagt gggccgacac aatgaccgcg agctacgacg ccctcgccga
     1921 caagctgccg gttttcgccg agctgcgcaa ctgcatggac ctcgccgtcg tcggggcatt
     1981 gctcgtcaaa gaggacttgc ccggccgcgc cggctgcgat ctgagcctgc tgctggaaga
     2041 caaacgcgtc gccgtggccg agtatcacgt gcccaagaca atcgactcgc gggccagcc
//
LOCUS       DASUGB010000581         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_620579, whole genome shotgun sequence.
ACCESSION   DASUGB010000581 DASUGB010000000
VERSION     DASUGB010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620579"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VFV87_11120"
     CDS             <1..115
                     /locus_tag="VFV87_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX5104355.1"
                     /translation="DQFILRLRKIFETDPTQPRHFLTIRDLGYRFVSQPEA"
     gene            complement(172..849)
                     /locus_tag="VFV87_11125"
     CDS             complement(172..849)
                     /locus_tag="VFV87_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX5104356.1"
                     /translation="MSSIYQDVLGSDFERLHPQIQRRFGFSSADGVAATGSGVMETLW
                     HGPQYTLPFLYLGTWRRIMFPEQGTNVPFTIQNYAYRDPLGRETVTWIRTFQTSRPRR
                     FDAYMIHSRRRDRIVDYLGTHQHLAVDLELSVDERGGLRLRSGEQRFYEGPLAFRFPL
                     FFSGIADVCEWYDESLGQFRIEVNVSNRFWGPLFGYRGTFNVEWKAIQPTEIPKEVLP
                     RRIERRE"
     gene            complement(849..1742)
                     /locus_tag="VFV87_11130"
     CDS             complement(849..1742)
                     /locus_tag="VFV87_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HEX5104357.1"
                     /translation="METRIRAPLDELWRLTQSPDLHARWDLRFTRIQYLPRLDEYQPQ
                     RFLYATRIGCGWAIEGAGETVGQRCGEHGERASALKFWSDDPKSLIREGAGYWRYVPA
                     DGSVRFFTSFDYRVRFGALGQVIDTALFRPLIGWATAWSFDSLRLWLEKGLDPATSRE
                     RSLIHLMARAALAFVWIYQGTVPKLLTQNADELAMLQQTGLSAPDSLVAIQVIGWAEV
                     AFGLLILLRLRDRWPLVLTILLMLAATVSVALSSPHYLTMAFNPISLNLLMVAVAAIE
                     FLVSRDLPSASHCLRVPSEDR"
     gene            complement(2178..2720)
                     /locus_tag="VFV87_11135"
     CDS             complement(2178..2720)
                     /locus_tag="VFV87_11135"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX5104358.1"
                     /translation="MNTPFAVSTHIFPSDLGWMALAWTGKGLARFTFGHPSAAAAIAS
                     LEADGEWTTARASDLPQWVSDLAGRLAAYAAGNAEQFGDVPLDLSHLTAFQRKVVARC
                     RQISRGKTRSYGELARAIGSPGAARAVGSVMAKNRFPIIVPCHRVVGAAGSLGGFSAP
                     DGLGMKRRMLALEGVTREKP"
     gene            2935..>3339
                     /locus_tag="VFV87_11140"
     CDS             2935..>3339
                     /locus_tag="VFV87_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104359.1"
                     /translation="MKDRGSRQGRYHSFCDDYYVNMNLNTEMDLPQSRETVLHFFEQV
                     KKTYPTMRNFYGRERGEFVLEEDKDQGHYRWVSVETRRVSSGFVNPNNVEEALKQHSQ
                     ILDMIPYALSISPLDCESLNVMYGFDYTYRGNH"
BASE COUNT          673 a         1068 c         1008 g          590 t
ORIGIN      
        1 ggatcagttc atcctccgcc tccgcaagat cttcgagacc gaccccaccc agccgcgcca
       61 tttcctcacc atccgcgacc tcggttatcg cttcgtctcc cagccagaag cgtaggacgg
      121 ccgcatccgc cgatcgaagc aaagtgagca ctgcgctggt caagtgattc gctactcgcg
      181 ccgttcgatg cgccgcggca gaacttcctt gggaatctcc gttggctgaa tggccttcca
      241 ttccacgttg aatgtgccgc gatagccgaa aagtggaccc cagaagcggt tagagacatt
      301 gacttcgatg cggaattgcc ctagcgactc gtcataccat tcgcacacgt cggcgatgcc
      361 agagaagaac agcggaaagc ggaatgccag cggcccttca taaaaccgct gctcgcccga
      421 acgcagccta agcccgcccc gctcatcgac cgacagctcc agatcgaccg ccaaatgctg
      481 gtgcgtgcca agatagtcca caatccgatc gcgccggcgg ctatggatca tgtaggcgtc
      541 gaaccgtcgc ggtcggctgg tctgaaaggt gcgaatccac gtcaccgtct cgcggcctag
      601 cggatcgcgg taggcgtaat tctggatcgt aaacgggacg ttggtcccct gctccggaaa
      661 catgatccgc cgccacgtgc cgagatagag aaatggcagg gtgtactgcg ggccgtgcca
      721 gagtgtctcc ataacgcccg agccggtcgc cgccacgccg tcggcactgg aaaatccaaa
      781 acgccgctga atctgcggat gcaggcgttc gaagtccgag ccgagcacgt cttggtaaat
      841 ggaactcatc acctgtcctc cgacggcacg cgcagacaat gactcgccga cggcaagtcg
      901 cggctgacca ggaattcaat cgccgcgacg gcgaccatga gcaagttcag gctgatcggg
      961 ttgaacgcca tcgtcaggta gtgcggcgaa ctcagggcga cgctcaccgt tgccgcgagc
     1021 atgagaagga ttgtcagcac cagcggccag cggtcgcgaa gtcgcaaaag aatcagcagg
     1081 ccgaaagcaa cctccgccca gccgatgact tgaatcgcaa ccagcgagtc cggcgcgctc
     1141 aagccggtct gctgcagcat cgccagttcg tcagcattct gagtcagcag cttgggcacg
     1201 gtcccctgat agatccacac gaatgccagt gccgcgcgag ccataaggtg aatcaagctc
     1261 cgctcgcgcg acgtcgcagg atcgagccct ttttcgagcc acagccgcag gctgtcgaag
     1321 ctccacgccg tcgcccagcc tattaggggc cgaaagagag ctgtatcgat gacttggccc
     1381 agcgcgccga aacggacccg gtagtcaaat gacgtgaaaa accgcacact gccgtccgcg
     1441 ggaacataac gccaataccc cgcaccttcg cgaatcagcg atttcgggtc gtcggaccag
     1501 aacttcagtg ccgacgcccg ctcgccgtgt tcgccgcagc gctgcccgac cgtttctcct
     1561 gcaccttcga tcgcccatcc gcagccgatc cgcgtggcgt aaaggaaccg ctgtggctgg
     1621 tattcatcca gccgcggcag gtactggatg cgtgtgaacc gcaagtccca gcgagcgtgg
     1681 agatcaggcg actgtgtcag tcgccacaat tcgtccagcg gcgcgcggat tcgcgtttcc
     1741 acatagatgg caatggcacg tttccttgag cgagtactgc tctcctaccc gccggatcgg
     1801 cacaaacgta accagggcgc gcggagtttt ttcctccacg agcatttccg cgcagaatcg
     1861 gtcaggggag cattcggtga gccgccgaga cgattacccg aaggcggcct aatgcgggcc
     1921 ggaaaaggac gtcgattttg tcaccgctca acaatcaagc gcctgcccat ttatgtttct
     1981 ggcagaccgc agtttgcagt cgcaagcatt ctgcgggacc tgcctgcgag cggcgggccg
     2041 tcgataaaca gagtattcgg cctagccgcc ggatgcagca cgcttgccgc tcgccgtttc
     2101 gcgcgagcga cgagaccgcg gctgggcggg gtcggtctcg aagatcttgc ggagcccttc
     2161 cgagttttct gtgtgttcta tggtttctcc cgcgtcacgc cctccagcgc cagcatgcgg
     2221 cgcttcatgc ccaggccgtc cggcgccgag aagccgccga gcgagccggc cgcgccgacg
     2281 acgcggtggc aggggacgat gatcggaaag cggttcttgg ccatcacgct gcccacggcc
     2341 cgcgccgcgc cgggggaccc gattgctctg gccaactcgc cgtagctccg cgttttgccg
     2401 cggctgatct ggcggcagcg agcgacgact ttccgctgga aggcagtcag gtgcgaaagg
     2461 tcgagtggca cgtcgccgaa ctgctcggca ttgcccgccg catacgccgc cagccgcccg
     2521 gccaggtcgc tgacccactg gggcagatcg ctcgcgcggg cggttgtcca ttcgccgtcc
     2581 gcctccagcg aggcaatcgc cgccgcagcc gagggatggc cgaacgtgaa cctcgccagc
     2641 cccttgccgg tccaggcgag tgccatccag ccgaggtcgc tggggaagat gtgggtggaa
     2701 acggcaaatg gcgtattcat gggcggttct ccttcgttta acgcgtagcc gaagcgaagc
     2761 ggaggcgtag ccgcgattgg gatggaagcc gcattcggct acgcctgcgg ctgcgcgtta
     2821 aacgaagttt tggcatccac agcgccgctt tgacagcccg gcgcgcggcc acgtagaata
     2881 gacgaaaatt gtacaatcgc cctcagctcg cgccagcggg caggagctct ctcaatgaag
     2941 gaccgaggat cacgtcaggg tcgctatcac tcgttctgcg acgactacta cgtgaatatg
     3001 aacctcaata cggagatgga cctcccccag agccgcgaaa cggtgctgca tttcttcgag
     3061 caggtgaaaa agacgtaccc gacgatgcgg aacttctatg gccgcgagcg gggcgagttc
     3121 gtgttggaag aggacaagga ccaggggcac taccgctggg tgagcgtcga gacccgccgc
     3181 gtttcgtcgg gcttcgtcaa cccgaacaac gtcgaggagg ccctcaagca gcattcgcag
     3241 atcctggaca tgattcctta tgccctctcg atcagcccgc tcgattgcga atcgctgaac
     3301 gtgatgtacg ggttcgatta cacctatcgc ggcaatcac
//
LOCUS       DASUGB010000582         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_64850,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000582 DASUGB010000000
VERSION     DASUGB010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64850"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFV87_11145"
     CDS             complement(<1..977)
                     /locus_tag="VFV87_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104360.1"
                     /translation="MCSRESRVDFAWRLVLILASLLATARIGGADDWPLADRTSHLPQ
                     PWIVVRLSAELFEPLVEQPIDETVPVEELILGTQVAGQAHVAGRPELVLAEDATSAAF
                     TVRLAGTIKSQTTGRMGPITIFGGAETSFTASKRVVFDPAGGFVGQRPAIEVQTNVAT
                     DRIQSRRGGPVGRALERVAWSRVESTKDEVAAIVREKTECQLGAEFDRMLEERLALLN
                     RAASLRHLAELLVGGPDRVQYVCCTTPRGLQIAALVGSGQPAEAARRLALGAPRAFGL
                     PVTVCVHESMVSQRAATALRWLDIGRRMVDLPGPLHLPGTRQLDGPPLAV"
     gene            complement(1201..>2107)
                     /locus_tag="VFV87_11150"
     CDS             complement(1201..>2107)
                     /locus_tag="VFV87_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5104361.1"
                     /translation="HAVAFAAGQAKAGLRPIVDIYSTFLQRSYDQIFQEVALQNLPVV
                     FTLDRAGLTGPDGPTHHGCFDLAYMRCLPNLVVMAPGDAWDVPAMLDFALAHGGPIAL
                     RYPKANAYCITATSGREQCITATSGREEYAAATSGRADLPSDSRPMVAAMHSDRQPVE
                     LGRSEAIRPGCDGTILCLGTLLAECLKAAQSLAAEGVDVAVVNARFAKPIDREMVERA
                     IRETGFVLTVEEGCLLGGFGSAVLETASDLGLDASRVRRLGIPDQFIEHGERGELLAD
                     LHLDAAGIAAACRELAAQGQGIGHS"
BASE COUNT          358 a          753 c          671 g          325 t
ORIGIN      
        1 acggccagcg gcggcccatc aagctgccgc gtgccgggca aatgcagcgg accaggcaaa
       61 tcaaccatcc ggcgaccgat gtccagccag cgcagggccg tcgccgcgcg ctggctgacc
      121 atcgattcgt gtacgcacac cgttaccggc aggccaaacg ctcggggcgc gcccagcgcc
      181 aggcgacgag ccgcttcggc cggctgcccg cttcccacga gcgcggcgat ctgcaagccg
      241 cggggcgtgg tgcagcacac gtactgcacg cggtcgggtc cgccgaccag cagttcggcc
      301 aggtgccgca gacttgccgc ccggttgagc agcgccagcc gctcttcgag catgcggtcg
      361 aattcagcgc caagctggca ctcggttttt tcccgcacga tggcggcaac ttcgtccttc
      421 gtggactcga cgcgcgacca ggcgacccgc tccagcgccc tccccaccgg cccgccgcgc
      481 cgcgactgga tgcggtccgt cgccacgttg gtctgcactt cgattgccgg cctttggccg
      541 acgaatccgc cggccggatc gaagaccacc cgcttgctgg cggtaaagct cgtttcggcg
      601 ccgccaaaaa tcgtaatcgg gcccatccgt cccgtggttt gcgacttaat cgttcccgcc
      661 aggcgaaccg taaacgccgc gctcgtggcg tcctcggcca gcaccagctc gggccggccg
      721 gccacgtggg cctggcctgc cacctgcgtg cccaaaatca gctcctcgac cggcacggtt
      781 tcgtcgatcg gctgctcgac cagcggctca aacagttcgg ccgaaagccg caccacaatc
      841 caaggctgcg gcaagtgcga ggtgcgatcc gcgaggggcc agtcgtcggc cccgccgatt
      901 cgcgcggtgg cgagcaggct cgccagaatc agaaccagcc tccatgcgaa atccactcgg
      961 gattcgcgag agcacatgac gcccagcctt tgggccgcgg cggccagcaa ggccccaggc
     1021 cgtcaacgtg gcagtctgtt ccagccacgc cggcacgccg caggcaccag acgaagagcg
     1081 cccgcccgcc gcccgctccg cagcaggaat cgcaattcgc ctatttcaac catactttgc
     1141 aaaccgactg atggcgaatc gttttggcga tggcgtggga cttatccgct ccgttggcct
     1201 ctagctgtgc ccgatcccct gtccctgcgc ggccagctcg cggcaggcgg cggcgatgcc
     1261 ggcggcatcc aaatgcaggt cggccagcag ctctccccgc tcgccgtgct cgatgaactg
     1321 atcgggaatg cccaggcgcc tcacgcggct cgcatcgagg cccagatcgc tggccgtttc
     1381 gagcactgca ctcccgaagc cgcccagcaa gcagccttcc tcaaccgtca gcacgaagcc
     1441 cgtttcgcga atcgcccgct ccaccatttc gcggtcgatc ggcttggcaa accgggcgtt
     1501 gaccacagcc acgtccacgc cctcggccgc cagcgactgg gccgccttga gacattcggc
     1561 aagaagcgtt cccaggcaga gaatcgtgcc gtcgcagccg gggcgaattg cttccgagcg
     1621 accgagctcg accggctggc ggtcggagtg catcgccgcg accatcggtc gcgaatcaga
     1681 aggcagatcc gcgcgaccac tggtcgcggc tgcatattct tcgcgaccac tggtcgcggt
     1741 tatgcactgc tcgcgaccac tggtcgcggt tatgcagtag gcgttggcct ttggatagcg
     1801 cagggcaatc gggccgccgt gagccagggc gaagtcgagc atcgccggca cgtcccaggc
     1861 gtcgcccggg gccatgacca cgaggttcgg caggcagcgc atgtaggcca ggtcgaaaca
     1921 gccgtgatgc gtcggcccgt cggggccggt aaggccggcc cggtcgagcg tgaagacgac
     1981 cggcaggttt tgcagggcga cttcctgaaa aatctggtcg tacgagcgct gcaagaacgt
     2041 gctgtagata tcgacgatcg gccgcagccc cgccttcgcc tggccggcag cgaaggcgac
     2101 agcgtgc
//
LOCUS       DASUGB010000583         5592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_760414, whole genome shotgun sequence.
ACCESSION   DASUGB010000583 DASUGB010000000
VERSION     DASUGB010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760414"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..978)
                     /locus_tag="VFV87_11155"
     CDS             complement(175..978)
                     /locus_tag="VFV87_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5104362.1"
                     /translation="MTLVNLGCGRRIHPAWQNLDLTASVPGVIEHDLRRGLPFADNTC
                     EAVYHSHVLEHLEPEEGRGLIAECFRVLRPGGVLRVVVPDLEGITKQYLASLDAADSE
                     QAAKADHEWMTVELLDQMVRTRSGGRMRRYFDDAHLANRPFIVSRLGSEVTGEPAMAG
                     SKTLATRVKRSLSRLQRTAALAAATLVGGKQGYRDLKEVFFRRSGEVHRWMYDRVSLA
                     RLLAEAGFGRIARRTAFESAIPKFASYELDTTSDQVRKPDSLFMEGVKS"
     gene            complement(989..1894)
                     /locus_tag="VFV87_11160"
     CDS             complement(989..1894)
                     /locus_tag="VFV87_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5104363.1"
                     /translation="MGQDQSLDGFLAGGPLLAGQAELCRRRRFGYDIRMLLPSAVIRG
                     AWSRKGPAGRLLAACELKRIKRLPRYRPFRTRLLGPWLQGVDGPSFYYAFREIFEKRN
                     YEFTCTSAAPRIIDGGANIGLSVLFFKQLFPAARIVAFEPDATVFAALAENVLSFDLH
                     DVELVNQALWVRDQRLTFYSEGADAGRLRCAHERGVSVQVQACRLRDYLAEPLDMLKL
                     DIEGAEVDVLLDCADRLDGVQSLYVEYHSFAGQPQRLDELLAILRNAGFRIQIHTQFA
                     SHRPLVSRATTLGMDFQADVCAYRE"
     gene            1910..2941
                     /locus_tag="VFV87_11165"
     CDS             1910..2941
                     /locus_tag="VFV87_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5104364.1"
                     /translation="MIRGRFPSRFLLPGRIMPLETPVALLLYNRPQHTASVLAAIAAA
                     QPRKLLVVGDGPHTDREGDAERVALARAVIERVDWPCEVLTNYAPENLGCQRRVSSGL
                     DWVFGLVEEAIVLEDDCVPDPSFFAYCQALLERYRNDERIASIGGANFAEASHRTRDS
                     YFFSKYFFCWGWASWRRAWQRFDVTMRDWPAWKQNGGLASFAESSAELKYWKRALDAV
                     HGGKTDSWALPFALASFRHGGLNVVPAVNLVSNTGFGDDSTHTRCARHPLANLPVRAL
                     GTLQHPATVARNAPADRALFSRCYLPPRSSPWQRLWNSTVRRWAGVRAMWNSTRQSQR
                     PPVSPRRAA"
     gene            complement(2966..3889)
                     /locus_tag="VFV87_11170"
     CDS             complement(2966..3889)
                     /locus_tag="VFV87_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HEX5104365.1"
                     /translation="MSRGVVMMSKLGQLGRFANQLFQYMFLKTYALRHDLEVQTPPWI
                     GQQLFGLADPPIAGPLPEFVELYDKDPQRMVVPRLTEPLRNVDFSGFFQYPTRYYAPQ
                     REYIRRLFTPVPEAKQPLDAAVTRLRERGRTLVALHIRRGDYGFRYFYVTPLAWYLGW
                     LEQMWPTWESPLLIVATDEPAAVLPAFAAYRPVTAADLGLKLPIAPYFPDFYLLTQAD
                     VLAIPNSSFSFAAAMLNANLKAAWRSHLADPLGDPPFRTFDPWDADVLETGACAEDFP
                     HISGITRPPKRKWWRVERALSGLFRSRARAA"
     gene            4089..4745
                     /locus_tag="VFV87_11175"
     CDS             4089..4745
                     /locus_tag="VFV87_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104366.1"
                     /translation="MGQVRMGPPEELVLHLRDQAAVSTFVETGTFQGRTTRWAARHFQ
                     RVHTIEREPALHEAAKLRLEPYQHVTCHLGHSREVLAGLAPSLTTPCLFWLDAHWSGG
                     VTAGNDDECPLLEEIAEIDRHNQQHLLLIDDARLFLAPPPPPHRPESWPSIDAVCRAL
                     LARGEAYVCVHDDVILRVPRRWQHAVVEFFRRNTRGTPSPANPARNKGLLSWFGSRAA
                     "
     gene            4759..5466
                     /locus_tag="VFV87_11180"
     CDS             4759..5466
                     /locus_tag="VFV87_11180"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5104367.1"
                     /translation="MEDCRPATNGELRFAQLLLPSCRIVFDVGARQDDDLVRLNAGCA
                     FHLFEPSPKHCRALAEKVKDRSNVTVNPCAFGNARGTAWIYPDTESLVERAHSKSQPV
                     PVTLTRLDDYCREHNIRQIDFLKIDTEGYELEVLKGGRDIVEKGTRVIQFEYGGTYAD
                     ARITLRMVFDLLGPAWSFYRIYPDHLRPIRGYRPRLEDGRYANYVASRQPLSPAIVGP
                     GTLQRLFSGARWWRKAS"
BASE COUNT         1026 a         1799 c         1797 g          970 t
ORIGIN      
        1 cgaccccttg cttgcctcag acaacacgcg gagcgacgac gaactaccct cgccgcacac
       61 catcgatcag gccttgcagg atgaagactg gctcagcagc ctgaccgagc cgctgtgagg
      121 cccgccatac taaaccgatg cgtcagcgag ggactccctc gctgacgcgt cgggttacga
      181 tttcacccct tccatgaaca gcgagtccgg cttgcggacc tgatcgctgg tcgtgtcaag
      241 ctcgtagctg gcgaacttcg gaatggcgct ctcaaacgcg gtgcggcggg cgattcgtcc
      301 aaagccggct tccgcgagca gtcgtgccag cgagacccgg tcgtacatcc agcggtggac
      361 ttcgcccgag cggcggaaga aaacctcttt caggtcgcgg tagccctgct taccgcccac
      421 cagcgtcgcc gccgccaaag ccgccgtccg ctgcagccgc gaaagcgagc gcttgacgcg
      481 cgtggccagc gtcttggagc ctgccatggc cggttcgccg gtcacttcgc tgccgagtcg
      541 cgacacgatg aacggccgat tggcgaggtg ggcgtcgtcg aagtaccgcc gcatccggcc
      601 gccggagcgg gtgcggacca tctggtcgag caattcaacc gtcatccact cgtggtcggc
      661 cttggccgcc tgctcgctgt ccgctgcgtc caagctcgca agatattgtt tcgtaatccc
      721 ttccagatcc ggaaccacaa cccgcagcac gccgcccggc cgcaggacgc gaaagcactc
      781 ggcaatcaag ccgcgcccct cctcgggctc caggtgctcc aaaacgtgcg aatgatagac
      841 ggcctcgcac gtgttgtccg caaacggcag cccccgccgc aggtcatgct cgatcacacc
      901 cggcacgctg gccgtcaggt caaggttttg ccacgccgga tgtatccgcc ggccacagcc
      961 cagattgacg agcgtcatgc tggccctttc attcgcggta ggcacaaacg tcggcctgaa
     1021 agtccattcc cagcgtcgtc gcccggctga cgagcggtcg atgcgaggca aactgcgtgt
     1081 gaatctgaat gcggaatccc gcgtttcgca ggatggccaa cagctcgtcc agccgctgcg
     1141 gctggccggc gaacgagtgg tattcgacgt acaggctctg cacgccatcg agccgatcgg
     1201 cacaatccag cagcacatcc acctcggcgc cttcgatgtc gagcttgagc atgtcgagcg
     1261 gctcggcgag ataatctcgg aggcggcaag cctggacctg cacgctgacg cctcgctcgt
     1321 gagcgcaccg cagccgtccc gcatcggctc cttcgctata gaaagtcaag cgctggtctc
     1381 gcacccacaa tgcttgattg acgagttcca cgtcgtgcag gtcgaaagaa aggacgttct
     1441 cggcgagagc ggcaaatacg gtcgcgtccg gctcaaacgc gacgatccgg gcagcgggaa
     1501 agagctgttt gaagaacagg acgctcaggc cgatgttcgc tccgccgtcg atgatccgcg
     1561 gcgcggcgct tgtgcaggtg aattcgtagt tccgtttctc gaagatctcc cggaaggcgt
     1621 aatagaacga cggcccgtcc acgccttgca gccacggacc cagcaggcgc gtgcggaacg
     1681 ggcgatatcg cggcaggcgc ttgatgcgtt tgagctcgca agctgctagc agccgcccgg
     1741 ccgggccttt ccgggaccag gcgccgcgaa tgacagccga aggcaacaac atgcggatgt
     1801 catacccgaa gcgccgccgg cggcaaagtt cagcttggcc ggcgagcagc ggcccgccag
     1861 ccaggaagcc atccagcgac tgatcttgtc ccatgcggag cgaagggaca tgatccgagg
     1921 ccgattcccc tccagattcc tgctccccgg ccggatcatg cccctcgaaa cgcccgtcgc
     1981 cctgctcctc tataaccggc cgcagcacac ggcgagtgtc ctggcggcaa tcgcagcggc
     2041 tcagccgcgg aagctgttgg tggtcgggga tgggccgcac accgaccggg aaggggatgc
     2101 ggagcgggtc gcactggcgc gggcggtgat cgaacgtgtg gactggccgt gcgaagtgct
     2161 cacgaattac gccccagaaa acctgggctg ccagcggcgc gtttccagcg gcttggactg
     2221 ggtttttggc ctggtcgaag aggcgatcgt gctggaggac gattgcgttc ccgatccctc
     2281 gttttttgcg tattgccagg cgctgctcga gcgctatcga aacgacgagc ggattgcgtc
     2341 gatcggcggg gcaaatttcg ccgaggcctc gcatcggacg cgcgacagct atttcttctc
     2401 gaagtatttc ttctgctggg gctgggcgtc gtggcgcagg gcctggcagc ggttcgacgt
     2461 gacgatgcgc gattggcccg cctggaagca gaacggaggg ctggcgagct tcgccgaatc
     2521 gtccgccgag ttgaaatact ggaagcgtgc gctggatgcc gttcacggcg gaaaaaccga
     2581 tagctgggcg ctcccctttg cgcttgcctc gttccggcac ggcggcctga atgtcgtgcc
     2641 ggctgtcaac ctggtcagca ataccggctt tggcgacgat tcgacgcata cccgctgcgc
     2701 gcggcatccg ctggcgaact tgcccgtgcg tgcgctggga acgctgcaac atccagcgac
     2761 ggtggcccgc aacgcgccgg cggatcgggc cttgttttcc agatgttatc ttccgccgcg
     2821 ctcgtcgccc tggcagcggc tgtggaacag caccgtccgc cgatgggccg gcgttcgcgc
     2881 catgtggaat tccacccgcc aatcgcagcg cccgccggtt tcgcccaggc gtgccgcctg
     2941 acaggacgcg acggtccaga agccgctagg cggcgcgggc ccgtgagcgg aacaagcccg
     3001 acagtgccct ctccacgcgc caccacttgc gcttaggcgg cctggtgatg cccgaaatgt
     3061 gcggaaaatc ctcggcgcaa gcccccgttt ccagaacgtc cgcatcccag gggtcgaacg
     3121 tgcggaacgg agggtcgccc agcggatcgg ccaggtgcga gcgccaggcg gctttcaggt
     3181 tggcgttcag catggcagcc gcgaaggaga acgagctgtt gggaatcgcc agcacatcgg
     3241 cctgggtcag caagtagaaa tccggaaagt acggggcgat cggcagcttc aagccaagat
     3301 ccgcggcagt gaccggccgg taagctgcaa aggcgggcag caccgctgct ggttcgtcgg
     3361 tcgcaacgat aagcaggggc gactcccagg tcggccacat ttgctccagc cagcccagat
     3421 accaggccag cggcgtcaca tagaaatagc ggaagccgta atcgccgcgc cgaatgtgca
     3481 gcgcgaccag cgtgcggcct cgctcccgca agcgagtcac ggcggcatcg agcggctgct
     3541 tggcctcggg aacgggcgta aacagccggc ggatatattc ccgctgcggc gcatagtagc
     3601 gggtcggata ctggaagaac ccggagaagt ccacgttccg cagcggctcc gtcagacgcg
     3661 gcacaaccat ccgctggggg tctttgtcgt agagctcgac gaactccggc aacggcccgg
     3721 caatcggcgg gtcggccagg ccaaagagct gttggccgat ccagggcgga gtctgaacct
     3781 ccagatcgtg ccgcagggcg tacgttttca ggaacatgta ctggaacaat tggttggcaa
     3841 agcgccccaa ttggcccagc ttgctcatca tgacaacgcc gcgtgacatt tcattctttc
     3901 gtgcagtgca gaaaatgcca cgcgagatcg taattcaagc ccgcagcccc cagcaagacg
     3961 aaacggtgtg gcctctggct gcgtgagtcg gcattgttgg caattggctc tggacgataa
     4021 acttcaccgc gctgcgatca cctggaatcg cggctgcgac gagggcgttc gcaaagggag
     4081 ttgccgacgt gggacaagta cgcatggggc cgccggagga attggtcctt catctgcgcg
     4141 accaggcagc ggtttccact tttgtcgaga ccggcacctt tcagggccgc acgacgcgct
     4201 gggctgcccg acactttcaa cgcgtgcaca cgatcgagcg cgagccggcg ctccatgaag
     4261 cggcgaaact gcggctggag ccctatcagc acgtgacctg ccacttgggc cacagtcgcg
     4321 aggtgctcgc cggcctggcg ccttcgttaa caacgccctg cctgttctgg ctcgacgcgc
     4381 actggtcggg cggcgtgacc gctgggaatg acgacgagtg cccgctgttg gaggaaattg
     4441 ccgaaatcga tcggcacaac cagcagcacc tgttgctgat cgacgacgcg cggctctttc
     4501 tcgcaccgcc accgccgccg caccggccgg aaagctggcc ctcgatcgac gcggtctgcc
     4561 gggcgctgct ggcccgcggc gaggcctacg tttgcgtcca cgacgacgtc atcctgcgcg
     4621 ttccgcggcg ctggcagcac gcggtcgtcg agtttttccg gcggaatacg cgcggcacgc
     4681 cttcgcccgc caatccggcg cgcaacaagg gactgctgtc ctggttcggc agccgggccg
     4741 cctagcggag aaagcgccat ggaggattgc cgccccgcga ccaacggaga attgcgattt
     4801 gcgcagctcc tgcttccctc gtgccgaatt gtcttcgacg ttggcgcgcg gcaagacgac
     4861 gatctggtgc ggctgaacgc gggctgtgca tttcacttgt ttgagcccag cccaaagcat
     4921 tgcagggcgc tggcagagaa ggttaaagat cgctccaacg tgacggttaa cccctgcgcc
     4981 tttggcaatg cacgtggtac cgcctggatt tacccagaca ccgaatcgct cgtggagcgc
     5041 gctcacagca agtctcagcc cgtaccggtc acgctgacgc ggctggacga ctattgccgg
     5101 gagcacaata ttcgccagat cgactttctg aagatcgaca cggaaggcta cgagctggaa
     5161 gtgctcaagg gaggccgcga cattgtcgag aagggcacgc gggtcatcca gttcgaatac
     5221 ggcggcactt acgccgacgc ccggatcacg ctccggatgg ttttcgacct gctcgggccg
     5281 gcgtggtcgt tctatcgcat ctatcccgat catttgcggc cgattcgcgg ctatcgcccg
     5341 cggctggagg acggccggta tgccaactat gtggcctcgc gccagccact ttcgcccgcg
     5401 atcgtcggtc cagggacttt gcaacggctg ttctcggggg ctagatggtg gaggaaggcc
     5461 tcgtgattat cgatctggag cgcgagctgc aaaagtatgc gctgccggtg cggacggcgc
     5521 tgcacatcgg cgcccatcac ggcgaggagg acgaactcta ccgcaagctg gccatcgagc
     5581 cggtctatgt cg
//
LOCUS       DASUGB010000584         7389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_64909,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000584 DASUGB010000000
VERSION     DASUGB010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64909"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFV87_11185"
     CDS             <1..803
                     /locus_tag="VFV87_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5104368.1"
                     /translation="TWLVDASTDSGWLFCYGTNLVISARRQWCFGPLDHFWSLAVEEQ
                     FYLLWPFVILWLRRRQAMLLCASLIVGASLGRFVWILEGGDEVTRYALTVFRMDTLAA
                     GAWIALYLRGGASVASCGRIATVALCTISLLLLPLSVLHLRLLSLTDVLWTAGCAALL
                     VAMLAAQPGTILDHVGKSRVLGWFGHYSYGLYVFGNLLIAALAPLITAAEMGRLFGNN
                     YVGQVVYVAVVGAATVAVAVLSWHLYEKHFLGLKALLANAAKRRPGIG"
     gene            complement(829..2163)
                     /locus_tag="VFV87_11190"
     CDS             complement(829..2163)
                     /locus_tag="VFV87_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104369.1"
                     /translation="MFSRCSLRLVCLLSLVSAPAASADFEAERAANWHQWRGPEATGV
                     APEGNPPFEWSESKNVKWKVEIPGHGKSTPIVWGNRVFVITAIDTGKVVEGQAKPEDQ
                     PDRPFGIKYPNTLFRFVVLCLDRENGKILWERTAAEELPHEGHHGDNSHATASPTTDG
                     RYLYVSFGSRGLYCYDLAGERRWKRQLPAVETRLSFGEGSSPVISGDVLLLNRDNETD
                     SHLLALNAQTGDILWQAQRDEISAWATPLVIEHGGRTQVITNASNRVRSYDLASGEVV
                     WECGGQVSNVTPSPVRFGDHVVCMSGYKGSSAMSLPLGASGDITGTDKIAWQYERDTP
                     YVPSPLLDGERLYFTKSNSAILTCLDVRTGKPILEAARLPGLSNIYASPVAAAGRIYL
                     TGRDGTTLVLKRADKLEVLATNKLDDAIDASPAIAGNQFFLRGRRWLYCLQE"
     gene            2335..4692
                     /locus_tag="VFV87_11195"
     CDS             2335..4692
                     /locus_tag="VFV87_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5104370.1"
                     /translation="MRDRLVPPWLLLPAMLLIAVFPAPAEAGRRSRRSSHAPHGAPTA
                     SAARKSMQLTYPATRRDEQTDEYHGTQVADPYRWLEDLDSRQTRAWVQAQNEVTFAWL
                     GQIGTRERIRQRLTQLWNYERYGLPHKRGGRYFYTRNDGLQNQSAVYVIDKLGSQPRM
                     LLDPNTLSSDGTIALANWFVSDDGRYLAYGLAAAGSDWQEWHVLDVASGRKLSDHLKW
                     LKFSRVSWLPDGSGLYYSRYDEPPPGETYTGANYYQKLYFHKLGDPQAQDTLIYERPD
                     EKEWGFDGDVTEDGRYLIISIWRGTERKNQVFYKDLQRPDSPVVELIAGWDAEYHFIG
                     NDGPKFWFVTDKDAPLRRVIAVDTGEPRPMVAALAKQRGRESIAATTGRLEEALPQLT
                     PDPLPPGAPERAAPVANPGNRTGWKEVIPESNETLQSASVVGEHFVLRYLKDATSAIR
                     VHDLTGKRLRDVELPGLGSAGGFGGRKDDPETFYSFTSFTAPGSIYRYDVATGKSTLF
                     REPKVDFDPSRFETRQVFYHSKDGTRVPLMIVSRKGLILDGCNPTILYGYGGFNVSQT
                     PNFAVSTIAWLEMGGVYAVANLRGGGEYGRAWHEAGMLDRKQNVFDDFIAAAEWLIDN
                     KYTSPQKLAIRGGSNGGLLVGAAMTQRPELFAAAVPAVGVMDMLRYHKFTIGWAWVNE
                     YGSSDDSQQLPTLLAYSPLHNLKPGTHYPATLITTGDHDDRVVPAHSYKFAAALQASQ
                     AARQPTLIRIETRAGHGAGTPTTKLIDAATDVLAFLSAVLQAPAP"
     gene            4949..6097
                     /locus_tag="VFV87_11200"
     CDS             4949..6097
                     /locus_tag="VFV87_11200"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104371.1"
                     /translation="MERILRRDLDELLVPTATPCVSIYIAFHLVGRESQQDAGRLRKL
                     IDEAEEQLVDRGMFRKAARALLEPARSLKSDAAAWQKRGRWMAMFLAPGIARMFQGSG
                     KMDEALFVDAAFHLRPLLPLVSERDHFFLLALSHNSVRMFEGNSGWLEPLALPDLPTT
                     LEAALNIDEGYPGFQVHAATRGGEGGKQGVVFHGQGGKPAEASTFLEAFVRRVAAVVD
                     RHLIQEKAPLVLATVAANVPVWKSVSEYKHTMDDFIAGCPDALGPAELHAAAWPLVQP
                     VLDEERDLLHRRITHAQGNVSLGLKDVVPAAMNGRVDTLFIDCTRPRWGHYDPEQQAI
                     ELHEKRENGDADLVELAAIETLRHRGQVYALARESNAPEPSAEALLRY"
     gene            6163..6600
                     /locus_tag="VFV87_11205"
     CDS             6163..6600
                     /locus_tag="VFV87_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5104372.1"
                     /translation="MSAGRICIREVDTADVTESVQSAADRMNARNVGTLVVLNPERQP
                     LGIVTDRDLTVRVLARGKDPLATTVGDVMTLFPRTVYEETSIEDAISLMRVGPFRRVP
                     VVDREGKLVGLLSIDDVLELLAEEFRDIGQLLKAERPEVLAKV"
     gene            complement(6650..7225)
                     /locus_tag="VFV87_11210"
     CDS             complement(6650..7225)
                     /locus_tag="VFV87_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104373.1"
                     /translation="MSAIGRDHIVRPGRKSDVRRVAQALQKAVEPTEKGQIELIDAYE
                     VRSEKLSLDYGVVSYNEVVRSGRSVSHSVGVGHLIVLALPCDLGRIWIRPEGLLDKLS
                     EFVVSGDIDFAEHPRFSSRYLLHADDPELLNRRVSAAFWQAFGEQRRLVAVGRGATLV
                     IGKDGGLHSDDAADLVALGFRLLRAFGSPVS"
BASE COUNT         1334 a         2362 c         2351 g         1342 t
ORIGIN      
        1 cgacgtggct ggtcgatgcc tccaccgatt ctggctggtt gttctgttat gggaccaatc
       61 tcgtcatctc ggcacgccgt caatggtgtt ttgggccgct cgaccacttc tggtctctgg
      121 ctgtcgagga gcagttctat ctcctttggc cgtttgtcat tctctggctg aggcggcgtc
      181 aggccatgct gctttgcgcc agcttgattg tcggggccag cttgggccgg tttgtctgga
      241 ttctcgaggg gggagacgaa gtgacgcgct acgctttgac tgtcttccgg atggacacgt
      301 tggcggctgg cgcctggatc gcgctgtacc tgcgcggggg ggcttcggtc gcgtcgtgcg
      361 gccggatcgc aacagtcgcc ctgtgcacaa tctcgctcct gctcctgccg ctctccgtgc
      421 tgcacctgcg actcttatcg ctgacggacg ttttgtggac ggccgggtgc gcagccctgc
      481 tcgtcgccat gctggccgcc cagccgggaa cgatcctcga tcacgtcggc aaaagccggg
      541 tgcttggctg gtttggccat tacagctatg gcctgtacgt tttcgggaac ctgctgatcg
      601 ccgcgctcgc gccgctcatt accgcggcgg aaatggggcg tctgtttggc aacaattatg
      661 ttggacaagt ggtttatgtc gccgtggtgg gggcggccac ggtcgcggtc gccgtcttga
      721 gctggcactt gtacgagaag catttcctcg ggctcaaggc actgttggcc aatgcggcga
      781 agcgtcgccc gggcatcgga taagcgccga gaacaaaacg cgcgtccatc actcctgcag
      841 gcaatacagc caccggcggc cgcgaaggaa aaactggttg ccggcgatgg cgggcgacgc
      901 gtcgatcgcg tcgtcgagct tgttcgtcgc cagaacctcc agtttatccg cccgcttaag
      961 gacgagggtc gtgccgtcgc ggccggtcag atagatgcga cccgcggcgg cgaccggcga
     1021 ggcgtaaatg ttcgacagcc ccggtaggcg cgcggcttcc aggatgggct tgccggtccg
     1081 cacgtccaga cacgtgagga tcgccgaatt cgacttcgta aaatacagcc gctcgccgtc
     1141 taatagcggc gacggcacat agggcgtatc gcgctcgtac tgccaggcga ttttgtcggt
     1201 ccccgtgatg tcgccgctcg cccctagcgg cagcgacatg gcgctgctcc ccttgtagcc
     1261 gctcatgcag acgacgtggt cgccgaagcg gacgggcgaa ggcgttacat tgctcacttg
     1321 cccgccgcac tcccaaacca cctcgccgct ggccaggtcg tagctccgta cgcgattgct
     1381 ggcattggtg atgacctgcg tgcggccgcc atgctcaatt accagcggcg tggcccaggc
     1441 gctgatttca tcgcgctgag cttgccagag gatgtcgccg gtctgggcgt tcagagccaa
     1501 caggtgcgaa tcggtctcgt tgtcgcgatt gagcagcagc acatcgccgc tgatgaccgg
     1561 cgaactcccc tcgccgaagc tcagccgcgt ttcgaccgcc ggaagctggc gtttccagcg
     1621 ccgctcgccg gccaggtcgt agcaatacag cccccgcgag ccgaacgaaa catagagata
     1681 gcggccgtcg gtggtgggcg acgccgtggc gtggctgttg tcgccgtgat gcccttcgtg
     1741 gggaagttcc tctgcggcag tccgctccca caaaatcttg ccgttctctc ggtccaggca
     1801 gagcacgacg aatcggaaca gcgtgttggg atacttgatg ccgaacgggc ggtccggctg
     1861 gtcttcgggc ttcgcctggc cttcaacgac tttgcccgta tcgatggccg tgatcacgaa
     1921 gacccggttg ccccagacga tcggcgtcga tttgccgtgg ccggggattt cgaccttcca
     1981 cttgacgttc ttcgactcac tccattcgaa cggcggattg ccctcgggcg ccacgcccgt
     2041 cgcctccggc ccccgccact gatgccagtt cgcggcccgc tcagcctcga aatcggcgga
     2101 ggcagcaggg gcggacacga gcgagagcag acagacgagg cgcaggctgc agcgtgagaa
     2161 catggcttct gtcaatcgtt gaggcggcaa tccacgaatt gcgagactgg catgttatca
     2221 tcggcccgcc attccggcga gttctgcatc gtccggccgg agcaaaaccc gccccaccat
     2281 gagctagact tgaagatccc cgattctgca ccttgatctg gaacttccgc gtccatgcgt
     2341 gatcgacttg tgccgccttg gctgctgctg ccggcaatgc tcttgatcgc cgtttttcca
     2401 gcgccggccg aggcgggccg ccgctcgcgg cggtcctcgc acgcaccgca cggcgctccg
     2461 actgcctctg ctgcccgaaa gtccatgcaa ctgacctatc ccgccacccg ccgcgatgaa
     2521 cagaccgatg agtatcacgg cacgcaggtc gccgacccgt atcgctggct ggaagacctc
     2581 gacagccgcc agacgcgggc ctgggtccag gcccagaacg aggtcacgtt cgcctggctc
     2641 gggcagatcg gcacccgcga gcgcatccgc cagcggctga cgcaactttg gaattatgag
     2701 cgctacggcc tgccgcacaa acgcggcggg cgctacttct atacgaggaa cgacggcctg
     2761 cagaatcaga gcgccgtgta cgtgatcgac aagctcggca gccagccgcg aatgttgctc
     2821 gatcccaata cattgagcag cgatggcacg atcgcgctgg ccaattggtt tgtcagcgac
     2881 gacggccgct atctggctta cggcctggcc gctgccggct ccgactggca agagtggcac
     2941 gtgctcgacg tcgcgagcgg ccggaagctt tccgaccacc tgaaatggct caagttctcg
     3001 cgcgtctcgt ggctgccgga cgggagcggg ctttactaca gccgttacga cgagccgccg
     3061 ccgggcgaga cgtacaccgg ggcgaactac taccagaagc tctatttcca caagctgggc
     3121 gacccgcagg cgcaggacac cctgatctat gagcggccgg atgagaagga gtggggcttc
     3181 gacggtgacg tgaccgagga cggccggtat ctgatcatca gcatttggcg cggcaccgag
     3241 cggaagaacc aggtcttcta caaggacctg cagcggcccg attccccggt cgtcgagttg
     3301 atcgccgggt gggacgcgga ataccacttc atcggcaacg acgggccgaa attctggttc
     3361 gtgaccgaca aggacgcgcc gctgcgacgg gtgattgcgg tggatacagg ggaaccgcga
     3421 ccaatggtcg cggctttggc caaacaaagg ggccgggagt caattgcggc cacaactggc
     3481 cggttggaag aggcgttacc gcaattgact cccgaccccc ttccgcccgg cgcgccagag
     3541 cgcgctgcgc cggtggccaa tcccggcaac cggaccggct ggaaggaagt cattcctgaa
     3601 tcgaatgaaa cgctccaatc ggcgagcgtc gtcggcgagc actttgtgct gcgttacttg
     3661 aaggatgcca catcggcgat ccgcgttcac gacctgacgg gcaagcgcct ccgcgacgtc
     3721 gagcttccag gacttggctc ggcgggtgga ttcggcgggc gaaaagacga ccccgagacc
     3781 ttctactcgt tcaccagctt caccgccccc ggctcaatct accgctacga cgtggcgacc
     3841 ggcaagagca ccctgtttcg cgagcccaag gtcgatttcg atcccagccg ttttgagacg
     3901 cggcaggtct tttatcacag caaagacggc acgcgcgtgc cgctgatgat cgtcagccgt
     3961 aaagggctga tactcgacgg ctgcaatccg acgatcctct acggctacgg cggcttcaat
     4021 gtctcgcaga ctccgaactt cgcggtcagc acgatcgcct ggctggagat gggaggcgtg
     4081 tacgcggtcg ccaacctccg cggcggcggg gaatatggcc gcgcctggca tgaagcgggc
     4141 atgctcgacc gcaagcagaa tgtgttcgac gactttatcg ccgctgccga gtggctgatc
     4201 gacaacaagt acacctcgcc gcagaagctc gccattcgcg gcggcagcaa cggcggcctg
     4261 ctggttggcg cggcgatgac ccagcggccc gagctctttg ccgctgccgt tcctgcggtc
     4321 ggcgtgatgg acatgctccg ctatcacaaa ttcaccatcg gctgggcgtg ggtaaatgag
     4381 tacggcagtt ccgacgattc ccagcagctt ccaaccctct tggcctattc tccgctccac
     4441 aatctgaagc cgggcacgca ctatccggca acgctgatta cgacgggcga ccacgacgac
     4501 cgcgttgtcc ccgctcacag ctacaagttc gccgccgccc tgcaagcctc ccaggccgcc
     4561 aggcagccga cgctgatccg catcgagacc cgcgccggcc acggcgccgg caccccgacg
     4621 acgaagctca tcgacgcggc gaccgacgtg ctggcctttc tttctgcggt tttgcaggcc
     4681 cccgcgccgt agggcgggat tgcccaccat caggtgcgcg gaattggatg agggaaaacg
     4741 gtggcagagc ccgctcgatg ggcttccgtg ccgcgtgaat cagccgctac atccggccca
     4801 ggatttgctc ggatttgctg tgagaagggt gaatcgcaag gcctttcgcg gagtaatgaa
     4861 ccgggcggca tgagcgaatc agagcagaat gagcgaatcc gcccagcgcc cgagcaagtg
     4921 aaacagttgg tgagattgga ggtgtcccat ggaacgcatc ttacggcgag atttggacga
     4981 gcttttggtt cccacggcca cgccctgcgt cagcatctac attgcattcc atctcgttgg
     5041 gcgcgagtcc cagcaggacg ccggacggct gcggaagctg atcgacgaag ccgaagagca
     5101 gctcgtggat cgtggaatgt tccgtaaagc ggcccgcgcg ctcttggagc cggcccgctc
     5161 gctgaagtcc gacgccgccg cctggcagaa gcgcgggcgg tggatggcca tgttcctcgc
     5221 ccccggcatc gcgcggatgt ttcagggcag cgggaaaatg gacgaggcgc tattcgtcga
     5281 tgcagcgttc catctccggc cgcttttacc gctggtctcc gaacgggatc actttttcct
     5341 gttggcattg agccacaatt cggtccggat gttcgaaggc aactccggct ggctcgagcc
     5401 gctggcgctg cccgacctgc ccaccaccct ggaggcggcc ctcaacatcg acgagggtta
     5461 tcccggcttc caggtccacg cagcgacgcg cggcggcgaa gggggcaagc aaggagtagt
     5521 cttccacggc caaggcggca agccggccga ggccagcacg ttcctggaag cgttcgtgcg
     5581 ccgcgtggcc gcggtggtcg atcggcatct gatccaggag aaggcgcccc tcgttctggc
     5641 gaccgtcgcg gcgaatgtac cggtgtggaa gtcggtctcg gaatacaagc acacgatgga
     5701 tgacttcatc gccggctgcc ccgatgcgtt aggcccggct gagttgcacg ccgcggcctg
     5761 gccgcttgtg cagccggtcc tggatgagga gcgtgacctg ttgcaccggc ggatcacgca
     5821 cgcccaggga aacgtgagcc tgggcctgaa ggacgtcgtt ccggcggcga tgaacggtcg
     5881 ggtcgatacg ttgttcatcg attgcacgcg gccccgctgg gggcattatg atcccgagca
     5941 gcaagccatt gagctgcacg agaagcgcga gaacggcgat gccgacctcg tggagctggc
     6001 cgcaatcgaa acgctgcgtc accgcgggca ggtctatgcc ctcgcgcgcg aaagcaatgc
     6061 ccccgagccc agtgcggagg ccctattgcg atattgagac ttcgtagggt gggaccagcg
     6121 ccagcgccgg cccacctgcg ctttgtaaaa ggaggtgacg ccatgtctgc cggaagaatc
     6181 tgcatccgcg aggtcgatac ggctgacgtg accgaatcgg tccagtcggc cgccgaccgg
     6241 atgaacgccc gcaacgtcgg gacgctggtc gtcctcaatc ccgagcgcca gccgctgggg
     6301 attgtgaccg accgcgacct gaccgtccgc gtgctggccc gcgggaaaga cccgttggcc
     6361 acgaccgtgg gagacgtgat gacgctcttc ccgcggacgg tctatgagga aacgtcgatc
     6421 gaagacgcga tctccctgat gcgcgtcggc ccgttccgcc gggtgccggt cgtcgatcgc
     6481 gaagggaagc tcgtgggcct gctcagcatc gacgacgtgc tggagctgct cgccgaggaa
     6541 tttcgcgaca tcggccagct cctcaaagcc gaacgaccgg aggtactggc gaaggtgtga
     6601 gcgtgtaggg tgggacaagg ccgccgcggc cgccggccca ccacgagcac tacgaaaccg
     6661 gtgagccaaa cgcgcgcagc aagcggaatc ctagcgctac aaggtcagcc gcgtcatccg
     6721 aatgaaggcc tccatctttc ccaatgacga gcgtggcgcc gcgcccgacg gcgaccagac
     6781 gcctttgctc gccaaaggct tgccagaatg cggctgacac gcggcgattc agcagctcgg
     6841 gatcgtcggc atgcaacaaa taccggctgc tgaagcgcgg atgctcggca aagtcgatgt
     6901 cgcccgagac gacgaactca ctcagcttgt ccagcagtcc ttccggccga atccaaatgc
     6961 ggcccaagtc gcagggaagc gcgagcacaa tcaaatgccc gacgccgacc gagtgcgaga
     7021 cactgcgccc gctccgcacc acttcgttgt acgagacaac gccgtagtcg agcgacaact
     7081 tttcagagcg cacttcataa gcatcgatca gctcgatctg tcccttttcc gtcggttcaa
     7141 cggccttctg caacgcctgg gcgacgcggc gtacgtcgga cttgcggcca ggccggacaa
     7201 tgtgatcgcg cccgattgca ctcacgcact cgcagacagc ttcatcaaat tgccgagaat
     7261 gcggcaacat aggcccagtt gccgacttac tttcggaagc cgtctctgca ctccagcctt
     7321 gggctgccag agcttgtgca tccggcttcc ctccgtcagg cgtgaagagc ctgtagtagc
     7381 gatgaaatc
//
LOCUS       DASUGB010000585         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_483333, whole genome shotgun sequence.
ACCESSION   DASUGB010000585 DASUGB010000000
VERSION     DASUGB010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483333"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3945
                     /locus_tag="VFV87_11215"
     CDS             <1..3945
                     /locus_tag="VFV87_11215"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX5104374.1"
                     /translation="GHAHAGCMLYQGGAWPEKYRGAVFMNNIHGNRMNIDLLEAQGSG
                     FVAHHGPDFLLTRDQWSQMLSMTYGPDGQVWVIDWYDMQQCHLREPNKHDRSNGRIYR
                     IAYDNAKPVKVDLQKLSDEELVKLLAHANQWYVRHALRILQERAARKPLADAIQSRLI
                     GLAVASDGATRVRGYWALHVTRDSIAMEQAPFSINAYEYAWRVRLPNERPWRESGSDE
                     RDAVWEGLKIRNAGSTVRLGIASAIQRLPLEDRWGALAAITEHEDAADHNLPLMYWYA
                     MEPLADVDPARALALGLSAGERIPILKEYMIRKIGAGDPAKSLALLVDGLKKAQDDAT
                     RLTFLRGMNESLKGRKGVNAPDDWQTVSAGLAKSDNLDIRFQSAVLSVNFGDEQAAKS
                     LAEMILNEKAGINFRKGAIQVLAQLQSQALVPVLVEALGASRNATEGVPYSAELRTDA
                     LRGLAAFDDARVPPAILGVYGELSPAERRDALTTLCARPAFAKALLDAVAAGKVPANH
                     LTADLVTNLRNLNDEALSKRIEQVWGIVRSSPEEKQKLIADYKRLLTQARSASEGPGG
                     GSLEFGRAIFAKTCANCHTLFGSGAKIGPDITGSQRANLDYLLTNLIDPSAVMAKEYQ
                     PLVVRLLDGRVVTGLKREETAETLALQTANELVVISKADIDESKQSDKSLMPDDQLLP
                     MSPHEVRSLVAYLGAGGQTPILATADNVGSFYDGKSLAGWVGDDKLWNVENGEIVGKT
                     AGLAKNEFLISELSVGDFKLELDVKLVEDKGNSGIQIRSQPIAGGEMRGYQADIGPGW
                     WGKLYEEHGRALLEAKGGEEFVKKGDWNHYEIIAAGSRVRTWLNGHLCVDRDDALGAR
                     SGVIAVQLHSGGATEVRYKNLKLTLISAPVPARPYPASKPTADGKPAKVSFKKTTLDR
                     AFRSEGVGMGDFNNDGLLDISAGSVWYENPGIQNPKSKIQNPATSWPMHVITEKPNSF
                     DIKTYGDTFMNWAEDLDGDGRQDLIVVDFPGKQTWWFQNPGTKGTGDGGQGTGGVGEP
                     WRRNVVVPVTNDESPLYLDIDGDGRRELIFGDAAQRMSIARPQSDPLQEWKATPISTK
                     GDAEIQRFYHGLGVGDINKDGRNDVIAPSGWWENVGRRSAAGQGSEEPWIFHPAPFGP
                     AQAQMYVFDFDGDGDNDVVGSSAHQRGIWWYEQASGGHEPAEASGDSRPALAEWTQHK
                     IDDSIAQTHALILADINGDGLPDLVTGKRYYAHNGRDPGEDEPPEIAWYELSRKAATS
                     GRGSAPAWTKHVIDADSGVGTQFEVHDMNADGLLDVIVANKHGVYYFEQVRE"
     gene            complement(3921..>4186)
                     /locus_tag="VFV87_11220"
     CDS             complement(3921..>4186)
                     /locus_tag="VFV87_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEX5104375.1"
                     /translation="NTPLADQVARGEVTLMERDDYIRTLVDFLELLPPEMIVERISGD
                     APPDYFVGPSWCLDKPATLRALQHELQCRDTWQGKGYSRTCSK"
BASE COUNT          865 a         1359 c         1309 g          653 t
ORIGIN      
        1 ggccacgccc acgccggctg catgctctat cagggcgggg cctggcccga gaaatatcgc
       61 ggcgcggtct tcatgaacaa catccacggc aaccggatga acattgacct cctggaagcc
      121 cagggcagcg gcttcgtcgc ccaccacggg cccgacttcc tgctgacccg cgaccagtgg
      181 tcgcaaatgc tctccatgac ctacggcccg gacggccagg tctgggtcat cgactggtac
      241 gacatgcagc aatgccacct ccgcgagccc aacaagcacg accgcagcaa cggccggatt
      301 taccgcatcg cctacgacaa cgccaagccg gtgaaggtgg atttgcagaa gttgagcgat
      361 gaggagttgg taaagctgct ggcgcacgcc aatcaatggt acgtccgaca tgcccttcgc
      421 atcctacagg agcgggctgc gaggaagccg ctcgcggacg ccattcagtc gcgtctcatt
      481 ggactcgcag ttgcgagcga tggcgctacg cgagttcgcg ggtactgggc gctacacgta
      541 acacgcgatt ccattgcgat ggagcaagct ccattctcga tcaacgccta cgaatacgca
      601 tggcgtgtgc gccttccgaa cgagcgcccg tggcgcgagt caggaagtga cgaacgcgac
      661 gccgtctggg aaggacttaa gattagaaac gctgggtcga cagtgagact tggtatcgct
      721 agcgcgatcc aaagacttcc gctcgaagat cgttggggag cgctggctgc aatcactgag
      781 cacgaagacg ccgcggatca caacctgccg ctgatgtact ggtatgcgat ggagccgctg
      841 gcggacgtcg atccggcgcg ggcgctggcg ctgggcctgt cggctggcga gcggattccg
      901 atcctcaagg aatacatgat ccgcaagatc ggcgcgggcg acccggccaa gtcgctggcg
      961 ctcctcgtcg atggcctgaa gaaagcccag gacgacgcca cgcgcttgac gttccttcgc
     1021 gggatgaacg aatcgctcaa gggccggaag ggcgtaaacg ccccggatga ttggcagacc
     1081 gtctccgccg gcctggccaa atccgacaat ctcgacatcc gcttccagtc cgccgtcctc
     1141 tcggtcaact tcggggacga acaggcagcc aagtcactcg cggaaatgat cctgaatgag
     1201 aaggctggga tcaacttccg caagggagca attcaggttc tcgcccagct tcagtcgcag
     1261 gcattggtcc cggtgctggt tgaggccctt ggcgcttccc ggaacgccac agagggcgtt
     1321 ccctacagcg cggagctgcg taccgatgcc ctccgcggcc tggcggcgtt tgacgatgca
     1381 cgggttcccc cagcgattct cggcgtctat ggcgagttgt cgcccgccga gcggcgcgat
     1441 gcccttacca cgctctgtgc ccggcccgcc ttcgcgaagg ccctgctcga cgccgtcgcc
     1501 gccggcaaag ttccggccaa tcacctcacc gccgacctag tcacgaacct ccgcaatctg
     1561 aacgacgaag cccttagcaa acgcatcgag caagtctggg gcatcgtccg cagctccccc
     1621 gaagaaaagc agaaactcat cgccgactac aaacgcctcc tcacacaagc ccgaagcgca
     1681 agcgaggggc cgggcggggg atcgctcgaa ttcggccgcg cgattttcgc caaaacctgc
     1741 gccaactgcc acactctctt cggcagcggc gcgaagatcg gccccgacat caccggctcg
     1801 cagcgggcga acctcgatta tctgctcacc aacctgatcg atccgagcgc cgtcatggcc
     1861 aaggaatatc agccgctggt cgtccgcctg ctggatggcc gcgtcgtcac gggcctcaag
     1921 cgcgaggaga ccgccgagac gctcgccctg cagaccgcca acgagctggt cgtcatatcg
     1981 aaagccgata ttgacgagtc gaagcaaagc gacaagtcgc tgatgcccga cgaccagctc
     2041 ctgccgatga gcccgcacga agtgcggtcg ctcgtcgcgt atctgggcgc cggtggccag
     2101 acaccgatac tcgccaccgc cgataatgtc ggcagcttct atgacggcaa gtcgctcgcc
     2161 ggctgggtcg gcgatgacaa gctgtggaac gtggagaatg gcgaaatcgt cggcaaaacg
     2221 gccggcctcg ccaagaacga gttcctgatc agcgagctat ccgtcggcga cttcaaactg
     2281 gaactcgacg tgaaacttgt cgaagacaag ggtaacagcg gcatccagat ccgcagccag
     2341 ccgattgccg gcggcgaaat gcgcggctac caggccgaca tcggccccgg ctggtggggc
     2401 aagctctacg aagagcacgg ccgcgccctc ctggaagcca aaggcggcga ggaattcgtc
     2461 aaaaagggcg actggaacca ctacgaaatc atcgccgccg gcagccgggt gcggacctgg
     2521 ctcaacggcc acctgtgcgt cgatcgcgac gacgcgctcg gagcacgaag tggcgtcatc
     2581 gccgtccagc ttcactcggg cggcgcgacc gaagtccgct acaagaacct caagctgacg
     2641 ctgatttccg cccccgttcc ggcccggccg tatcccgcat cgaagcccac cgcagacggc
     2701 aagccggcca aggtcagctt caaaaagacc accctcgacc gcgccttccg cagcgaaggg
     2761 gttggcatgg gcgacttcaa taatgatggc ctgctcgaca tctccgccgg cagcgtctgg
     2821 tacgagaacc ccggaatcca aaatccaaaa tccaaaatcc aaaatcccgc cacctcgtgg
     2881 cccatgcacg tcatcacgga aaagcccaac agcttcgaca tcaagaccta cggcgacacg
     2941 ttcatgaact gggccgagga cctggacggc gacggccggc aggacctgat cgtcgtcgat
     3001 ttccccggca agcagacctg gtggttccag aatccgggaa cgaaggggac aggggacggg
     3061 ggacagggga cagggggagt tggcgagccg tggagacgta atgtggtcgt gccagttacc
     3121 aacgacgaaa gcccgctcta tctcgatatc gatggcgacg gccggcgtga gctgatcttt
     3181 ggcgatgcgg cccagcggat gtcgatcgcc cggccgcaaa gcgatccctt gcaggaatgg
     3241 aaggccacgc caatttcgac caagggggat gccgagattc agcggttcta ccacggcctg
     3301 ggcgtcggcg atatcaacaa ggacgggcgg aacgatgtga tcgctcccag cggctggtgg
     3361 gaaaatgtgg gccgccgctc cgcggcaggc caggggagcg aggaaccgtg gatttttcac
     3421 cccgccccct tcggtccggc ccaggcccag atgtacgtct tcgatttcga cggcgacggc
     3481 gacaacgacg tcgtcggcag ctcggcccac cagcggggca tctggtggta cgagcaggcg
     3541 agcggcgggc atgagcccgc tgaggcctca ggggactcac gtcccgcgct cgccgagtgg
     3601 acgcaacaca agatcgacga ctcgatcgcc cagacgcacg cgctgatcct ggccgacatc
     3661 aacggcgacg gtttgccgga cctcgtcacg ggcaagcggt actatgccca caacggccgc
     3721 gacccgggcg aagacgagcc gccggaaatc gcctggtacg agctgtcccg caaagccgcg
     3781 accagtggtc gcggttcggc gccggcctgg accaagcacg tcatcgacgc cgacagcggc
     3841 gtcggcacgc agttcgaggt ccacgatatg aacgccgacg gcctcctgga cgtgatcgtc
     3901 gccaacaagc acggcgtgta ttacttcgag caggtacgag agtagccttt gccctgccag
     3961 gtatcgcggc actgcagctc atgctgcagg gcgcggagcg tggcgggctt gtcgaggcac
     4021 cagctcgggc caacgaagta gtccggcggg gcgtcgccgc tgatccgctc gacgatcatt
     4081 tccggcggca gcagctccag gaaatcgacg agcgtgcgaa tgtagtcatc acgctccatg
     4141 agcgtgactt caccgcgtgc aacctggtcg gcgagcggcg tgttcc
//
LOCUS       DASUGB010000586         8368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2011304, whole genome shotgun sequence.
ACCESSION   DASUGB010000586 DASUGB010000000
VERSION     DASUGB010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011304"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2233)
                     /locus_tag="VFV87_11225"
     CDS             complement(<1..2233)
                     /locus_tag="VFV87_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5104376.1"
                     /translation="MLRLRLDCRCLHLFLAGLTALGGMGICALGAEGGEISFDRDVMA
                     VLSKAGCNMGACHGNLNGKGGFKLSLRGQDSAADFESLVRGADQRRVNLLDPAASLIL
                     QKPTGQAVHQGGLRFKRDSLEYQILADWIAAGAPLPNAKSPRLKRLEVKPPSAVLIEP
                     VGEVQLMVTAYFSDGTQQDVTPLACYEATSRSVSIGAGGLVTCDQLGETTVLVRFLNQ
                     QLAVQLAFLPANPQFSFRSPQQAGSIDELVFAKLQTLRTNPSALADDTTFIRRATLDA
                     TGLLPTADEARDFVADVSPDKRERLIEQLLARPEFAEHWALKWSDLLRNEEKVLDSKG
                     VDLFYTWIRDWIAANRPMDEFVRELVAARGSTYDNPPANFYRANRDPLTRGETAARLF
                     LGVRLQCARCHNHPYDSWTQDDYYNWAAVFGRIDYQILANKRRDRLDKHEFNGEQVVL
                     IKDAGEVQNPRTGGDAQPTFLGGYSPPLHPAEDRLPPLADWLAAADNGQFVRAQVNFV
                     WYHLLGRGLVEPIDDFRVTNPPSNPALLDFLATDYASCGFDLQRMVRTIMTSRTYQLS
                     AEPNETNAADEANFSRAIVRRLPAETLLDAQFQAVGAVLEFNGYPVGTRAGQVKGTIR
                     VRSRDKRPASADRFLKTFGKPERLLACECERSNETTLKQAFTLIGDEGLNELLDDDDN
                     RLAGLARSELPASEIVADLYWSALSRAPTSEELAAGESLLQSADDRFVALRDLAWALL
                     NSKE"
     gene            2399..3310
                     /locus_tag="VFV87_11230"
     CDS             2399..3310
                     /locus_tag="VFV87_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX5104377.1"
                     /translation="MRVLVTGATGFIGHRLLAKLDAPVVLSRNAAKAKTTLKEFDVTA
                     FDWDPLKGSPPQAAFEGIDTVIHLAGDPVASGRWTKAKKARIKDSREIGTRNLVNGIA
                     ALTRKPSVLVSASAVGIYGDRGDEELDERSPPGDDFLADVCQAWEREAGRAAEIGPRV
                     ATIRIGIVLGEGGGALDKMLTPFKLGLGSPLGSGRQYMPWVHRDDLAELFLFAARERG
                     VHGPINGVAPNPVTNREFTKTLGKVLGRPTFMPSVPGFALKTMLGEFAEVLLASQRVV
                     PQAALAQGFAFKFRELEPALRDVLRKE"
     gene            3349..3816
                     /locus_tag="VFV87_11235"
     CDS             3349..3816
                     /locus_tag="VFV87_11235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5104378.1"
                     /translation="MAKSFILEREQLIRRPLADVFPFFADAANLQAITPEFLDFRFVT
                     PLPIEMRAGTLIDYQIRLCGVPLKWRTRIEEFDPPHRFVDVQVRGPYKSWHHTHEFHE
                     ADGGTLMLDHVRYELPLGPLGSLAAALFVRRLLNNIFDHRRRTIDSRMSPRTG"
     gene            3931..4761
                     /locus_tag="VFV87_11240"
     CDS             3931..4761
                     /locus_tag="VFV87_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5104379.1"
                     /translation="MTTSALGTLITYNPWTVAPASSLEEVANGFASLGVHHVAVVDDD
                     RRLVGIVSETDLLRARQGCALVAVGGPGDDRLELPALVVDVMSRGVRAISAHTTQHEA
                     LRLLVENRFHALPVADGERLIGMITSRDFLREFSYGDLPASRDLIVVRLAAAPQTVES
                     DTTLEEALLAMHETGSACLAVVTGGLPLGVVSQRDIVRARLRADENGAAGSSPTRATV
                     SQIVRSSPVIRPGQRLCDAAAAMIEHELPAVTVVNQASRLLGVITEDDILQVMYDGSR
                     "
     gene            4758..5435
                     /locus_tag="VFV87_11245"
     CDS             4758..5435
                     /locus_tag="VFV87_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5104380.1"
                     /translation="MRTWETLQWRIVACRRCPRLRDYCRQIGAEKKRAFADWDYWARP
                     VPNFGSPPAPLLIVGLAPAAHGANRTGRMFTGDRSGEWLYRALHKAGFANQPSSRERG
                     DGLELRDCVITAVCHCAPPDNKPLLNEIANCREWLTTTIDLVQPRVLLALGGIAWKAI
                     VSEARARGWHSGRVPSFGHGAKHALADGRWLFGSYHPSQQNTFTGKLTEPMFDSIFAT
                     ARRLIAG"
     gene            5522..7156
                     /locus_tag="VFV87_11250"
     CDS             5522..7156
                     /locus_tag="VFV87_11250"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5104381.1"
                     /translation="MSSIRAVCLGLLGLVLAANATGHDHTKKQFELQRALAVAQAKAQ
                     SEPAPREDADEKTCRLTIELRLDDAPQPIAGLVRITNLATGKAIAFADEIQREANWCA
                     VTSPATLVVPQGKLKVEALYGLESELVSREIDLTAQEKHLLTVDLQRFYKPADRKLVG
                     GNTHLHLMKLTHAEAVRYLETVPRADGLDLVFLSHLKRVPEDVDYISNLFTDGDLGRL
                     SKGGVLFGNGEEHRHNFGPGAEGYGHVMFLNLQRLVEPVSIGPGIMKSGTDGRPLRRG
                     IDEARSQAATVIWCHNQFGHEDLPNWIAGVLDAQNIYDGNPHGTYSDTYYRYLNLGLK
                     VPFSTGTDWFIYDFSRVYLPLEGELTARRWLLELAHGRSYITNGPFLELTAGQHKIGD
                     SIAAKAGDELPITARALGRSDFRGLELIHNGKVIEHVHGDKQNGHFEAELQFTLKIDE
                     PGWIAVRIPLEAGQNEFGKPHFAHTSPIYIELAGRRIFRPEVARGLIADMEKSIEAIK
                     AEGKFADAAEEEAVLSVYREGIAVMQQRLSQASTGQ"
     gene            7212..7886
                     /locus_tag="VFV87_11255"
     CDS             7212..7886
                     /locus_tag="VFV87_11255"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5104382.1"
                     /translation="MNAMLMSLPQPPTMEDVLERLGGVSPSRIRFQPLPGEATVQDVV
                     EIESRENRLFELVDGVLVEKVMGLRESILAAALAELLRRFVVPRNLGVVSGPDGMLQL
                     FPGLVRMPDVAYVSWQRIPGGEIPAEPAPLLAPDLAVEILSRSNTPAEMSRKLGEYFE
                     AGVSQVWFVDLDSRTITVYRSPDSADVHSVPAVIDGGSLLPGFSLPLAELFAELDRRG
                     NSPPGT"
     gene            7897..8025
                     /locus_tag="VFV87_11260"
     CDS             7897..8025
                     /locus_tag="VFV87_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104383.1"
                     /translation="MLQRPTRGAQTSQLDDPEEEAVLSVTREGIAAMRERIQENAR"
     gene            8022..>8368
                     /locus_tag="VFV87_11265"
     CDS             8022..>8368
                     /locus_tag="VFV87_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104384.1"
                     /translation="MSIHSKREEPADPNAIRPLSPTIRILVIVGELLLASLFLPLVAL
                     FPGVLVEREPGPFVVIGVGCLVAAYGLGLQRRWMVPASVAMVAISGYTLLRALDAISK
                     SAGWGVVLLTIVPL"
BASE COUNT         1496 a         2652 c         2687 g         1533 t
ORIGIN      
        1 actctttgct gttgagcagc gcccaagcca ggtcccgcag cgccacaaag cggtcgtcgg
       61 ctgactggag cagcgattcg ccggccgcta actcttcaga tgtcggcgcc cggctgaggg
      121 cggaccaata caagtcggca acgatctcgg acgccggcag ttccgaccgg gccaggccgg
      181 cgaggcgatt gtcgtcgtca tcgagcaact cgttcagccc ttcgtcgccg atcagcgtaa
      241 acgcctgctt cagcgtcgtc tcgttcgagc gctcgcattc gcacgcgagc agccgctcgg
      301 gtttgccgaa ggttttcagg aatcgatcgg cagaggcggg ccgcttgtcg cgcgagcgaa
      361 cgcggatcgt tcccttcacc tgcccggccc gcgtccccac cggatagccg ttgaattcca
      421 agacggcgcc gaccgcctga aactgggcat ccagcagcgt ctcggccggc agccggcgga
      481 cgatcgcccg ggagaaattc gcttcgtccg cagcattcgt ctcgttcggc tccgcggaaa
      541 gctgatacgt acggctggtc atgatcgtgc ggaccattcg ctgcagatcg aacccgcagg
      601 aggcgtagtc ggttgccagg aaatcgagca gcgcgggatt gctcggcggg ttggtcacgc
      661 gaaaatcgtc gatcggctcg accaggccgc ggcccagcag gtgataccag acgaaattca
      721 cctgcgcgcg gacgaactgt ccgttatcgg ccgctgcgag ccaatcggct aaaggcggca
      781 ggcgatcttc agccggatgc agcggcggcg aatagccgcc cagaaacgtt ggctgggcgt
      841 cgccgccagt ccgcgggttc tgcacttcgc cggcatcctt gatcagcacg acctgttcgc
      901 cgttaaactc gtgtttgtcg agccgatcgc ggcgcttgtt cgcgagaatc tggtaatcga
      961 tccgcccgaa gaccgcggcc cagttgtaat agtcgtcctg cgtccagctg tcatacggat
     1021 gattgtggca gcgggcgcac tgcagccgca cgccgaggaa cagccgagcc gctgtttcgc
     1081 cgcgagtgag cggatcgcga ttggcccggt aaaaattggc gggcggattg tcgtacgtgc
     1141 tgccgcgggc cgcgacgagc tcgcggacga attcatccat cgggcgattg gcggcaatcc
     1201 aatcgcggat ccacgtatag aacagatcga cccctttgga atcaagcacc ttctcttcgt
     1261 tccgcaatag gtccgaccat ttcagcgccc agtgctcagc gaattctggc cgcgcgagca
     1321 actgttcaat cagccgctcg cgtttgtcgg gtgatacatc ggcgacaaag tcgcgggcct
     1381 cgtccgccgt cggcagcagg cccgtcgcat cgagcgtcgc tcggcggatg aacgtcgtgt
     1441 cgtccgcgag cgcggacgga ttcgttcgca gcgtctggag cttcgcgaag accaactcgt
     1501 cgatggagcc ggcttgctgg ggcgatcgga atgagaactg cgggttcgcc ggcaaaaacg
     1561 cgagctgcac tgcgagctgc tggttcagaa agcgaaccag gacggtcgtc tcgccgagct
     1621 ggtcgcacgt gacgagcccg cccgcgccga tcgaaacgct gcggctggtg gcctcgtagc
     1681 aggcgagcgg cgtgacgtcc tgctgcgtgc catcggagaa ataggccgtg accatgagct
     1741 ggacctcgcc gacgggctcg atcaggaccg cgctgggcgg cttcacttcc agtcgcttta
     1801 gccgcggcga cttggcattc ggcaagggag cgccagccgc gatccagtcg gccagaatct
     1861 gatattccag cgagtcgcgc ttgaacctca agccgccctg atggacggct tgcccggtgg
     1921 gcttttgcag gatcaggctg gcagccggat cgagcagatt gacgcgccgc tggtcggcgc
     1981 cgcggacaag cgactcgaaa tccgccgcag agtcctggcc acgcagcgag agcttgaaac
     2041 cgcctttgcc gttcaggttg ccgtggcagg cccccatgtt gcagccggcc ttggagagca
     2101 ccgccatcac gtcgcggtcg aaggatatct cgccgccctc cgcgccgagc gcgcaaatcc
     2161 ccattcctcc tagggcggtc agtccggcca ggaacaggtg caggcatcga caatcgagac
     2221 ggaggcgcaa catgacgtat ccaagagtat caatgagtca gccttggttt gcaacgcagg
     2281 ctggcccgaa tcggcttctc cagcgcggct tcgaaaatcg acccaattgc cagacgcgtg
     2341 aatccggcca tagtgttgcg gttatgttcg cagcggcagg ccgcggaaag ggctgttcat
     2401 gcgcgtcttg gtgaccggag cgacggggtt cattggccat cggctgttgg cgaagctcga
     2461 tgcgcccgtg gtcctcagcc gcaacgccgc caaggcgaaa accacgctga aggagttcga
     2521 cgtcacggcc ttcgattggg atcctttgaa gggctcgcca ccgcaagccg ctttcgaggg
     2581 gatcgacacg gtgatccatc tggcgggcga ccccgtggct tccgggcgct ggacgaaggc
     2641 caaaaaggcc cgcatcaagg acagccgcga gatcggcacg cggaacctgg tgaacggcat
     2701 cgccgctttg acgcgcaagc cgagcgtgct ggtgagcgcc tcggccgttg gcatctacgg
     2761 cgaccgcggc gacgaggagc tcgacgagcg cagcccgccc ggcgacgact ttctcgccga
     2821 tgtctgccag gcgtgggagc gcgaagccgg ccgggccgcc gagatcggcc cgcgcgtggc
     2881 aacgatccgc atcggcattg tgctgggcga aggaggcggg gcactcgaca agatgctcac
     2941 gccgttcaag ctcggcctgg ggagcccgct cggcagcggg cggcaataca tgccgtgggt
     3001 ccatcgcgac gacctggccg agctgtttct gttcgccgcc cgcgaacgtg gcgttcacgg
     3061 gccgatcaat ggcgtggccc ccaatcccgt gacgaatcgc gagttcacca aaacactcgg
     3121 caaggtcctc ggccggccaa cattcatgcc cagcgtgccc ggctttgcgc tgaagaccat
     3181 gctgggcgaa tttgccgaag tgctgctcgc ctcgcagcgc gtggtgccgc aggcggcgct
     3241 cgcgcagggc tttgctttca agttccgcga gctggagccg gcattgcggg atgtattgag
     3301 gaaggagtga ggggtgagcg gtgagcggtg cggaggcggg tattcttgat ggccaaatcg
     3361 ttcatcctgg agcgcgagca actcattcgt cgtccgctgg ccgacgtctt tccgtttttt
     3421 gcggacgccg cgaacctgca ggcgatcacg ccggagtttc tcgatttccg cttcgtcacg
     3481 ccactgccga tcgaaatgcg ggcaggcacg ctgatcgact accagatccg gctgtgcggc
     3541 gtgccgctga agtggcggac gcggatcgaa gaattcgatc cgccccaccg gttcgtcgat
     3601 gtgcaagtac gcgggcctta caagtcgtgg catcacacgc acgagtttca cgaggcggat
     3661 ggcggaaccc tgatgctcga ccacgtgcgc tacgagttgc cgctcggtcc actgggctcg
     3721 ctcgccgcag cgctgttcgt gcgccggctg ctcaacaaca tcttcgacca ccgccgtcgc
     3781 accatcgatt cgcggatgtc cccccgaacg ggttagtcgt ggctcgccga catcggcacg
     3841 acaagcgacc tagaattgcc gggcgagttt gccgacccag ggatgaacct tcactccgac
     3901 aatgattctc gatcgctggt gagccctccg atgaccacct ctgctcttgg cacgctgatc
     3961 acgtataacc cctggaccgt ggcccctgcg tcctcgcttg aggaggtcgc caacggcttt
     4021 gccagcctcg gagtccacca tgtcgcggtc gttgatgacg atcgccggct ggtggggatc
     4081 gtttctgaaa ccgatctgct acgcgcccgc caggggtgtg ccttggtcgc ggtgggcgga
     4141 cccggcgatg accggcttga actcccggcg ttggtcgtgg acgtaatgtc gcgcggtgtt
     4201 cgggccattt ctgcccacac gacgcagcat gaggccctca ggctgctcgt cgagaatcgc
     4261 tttcacgcgc tgccggtcgc agacggtgag cggctgatcg ggatgatcac cagccgcgat
     4321 ttcctccgcg aattctcgta cggcgatttg ccggcgtcgc gcgatttgat cgtcgtgcgg
     4381 ttggcggcag cgccgcaaac tgtcgaatcc gacacgacgc tcgaagaggc gctgctggcg
     4441 atgcatgaaa cgggctctgc gtgcctggcg gtcgtaaccg gcggcctgcc gctgggggtt
     4501 gtctcgcagc gcgacatcgt ccgcgcccgc ctgagagcgg acgaaaacgg cgcggcggga
     4561 agttcgccaa ccagggctac ggtctcccag attgtccgtt cgtctcccgt cattcggccc
     4621 ggccagcgcc tgtgcgatgc tgccgcggcg atgatcgagc acgaattgcc ggccgtgacc
     4681 gtcgtcaacc aggccagccg cctgctgggc gtcattaccg aggacgacat cctgcaggtg
     4741 atgtacgatg gttcgcgatg agaacctggg agactctgca gtggcgaatc gtggcctgcc
     4801 ggcgctgtcc gcggctgcgg gactattgcc ggcaaattgg agctgaaaag aagcgggcgt
     4861 ttgccgattg ggactattgg gccaggccgg tgccgaattt cggttcgcct cccgcgccac
     4921 tgttgatcgt cggcctcgcg ccggcggcgc atggtgcaaa tcgcacgggc cgcatgttta
     4981 ccggcgaccg gagcggcgag tggctctacc gggctttgca caaggccggt tttgcgaacc
     5041 agccatcctc gcgcgagcgc ggcgatggcc tggagctgcg cgattgcgtc atcacagccg
     5101 tctgccactg tgccccgccg gacaataagc cgctgctgaa tgaaatcgcc aactgccgcg
     5161 agtggctgac gactacgatc gacctcgtcc agccgcgagt gctgctggcg cttggcggca
     5221 ttgcgtggaa agccatcgtc agcgaagccc gcgcacgcgg ctggcacagc ggacgcgtgc
     5281 cgtcgttcgg ccacggcgcg aaacacgctc tggccgacgg acgctggctg ttcggctcgt
     5341 atcatcccag ccagcagaat acgttcaccg gcaagctgac cgagccgatg tttgacagca
     5401 tcttcgcaac cgcacggcgg ctgatcgcgg gctgaagcgt ggggcgggcg tctcgccggc
     5461 ccgtcagata cacttgtgct tgaccattcc cgagagccca atcggacctg gagtaactgc
     5521 aatgagttct atccgcgccg tctgccttgg gcttctcgga ttggtgctcg cagcaaacgc
     5581 cactgggcac gatcacacga agaagcaatt cgagctccag cgggcgctgg ccgtcgctca
     5641 ggccaaggcc caatcagagc ccgctccgcg cgaagatgct gacgagaaga cctgccggct
     5701 gacaatcgag ctgagacttg acgacgctcc gcagccgatt gcgggactgg tgcgaattac
     5761 caacctggcg acgggcaagg ccatcgcctt cgcggacgaa atccagcggg aagctaactg
     5821 gtgcgcggtc acgagtcccg cgacgctggt cgttccgcag ggcaagctga aggtggaagc
     5881 actctacggc ctggaatccg agcttgtctc gcgcgagatc gacctgacgg ctcaggagaa
     5941 acacttgctc acagtcgatc tccagcgttt ctacaaaccg gccgaccgca agctcgtcgg
     6001 cggcaataca cacctgcacc tgatgaagct gacgcacgcc gaagccgtgc ggtatctgga
     6061 aaccgtgccg cgggctgacg gcctcgacct ggtgttcctc tcgcatctga agcgcgtccc
     6121 ggaggatgtc gattacatct ccaacctgtt caccgacggc gatctgggcc ggctgtcgaa
     6181 aggcggcgtg cttttcggca acggcgagga gcatcggcac aacttcggcc ctggagccga
     6241 aggttacggc cacgtcatgt tcctcaacct ccaacggctg gtggagccgg tgagcatcgg
     6301 gccggggatc atgaaatcgg gtaccgacgg ccgcccgctg cggcggggaa tcgatgaggc
     6361 ccgctcgcaa gcggcgaccg tgatctggtg ccacaatcag ttcggccacg aggacctgcc
     6421 aaactggatc gccggtgtcc tcgacgccca gaacatctac gacggcaacc cgcacggcac
     6481 gtattccgac acgtattatc gctacctcaa cctcggcctg aaggtgccct tttcgaccgg
     6541 caccgattgg ttcatctacg acttctcgcg cgtctatctg ccgctggaag gcgagctgac
     6601 cgcccggcgg tggctgctgg agctcgccca tgggcgctcc tacatcacca acggcccgtt
     6661 cctggagctg accgccggtc agcacaaaat tggcgactcc atcgccgcca aagcgggcga
     6721 cgaattgcca atcaccgccc gcgccctcgg ccggtccgat ttccgcggcc tggagctgat
     6781 ccacaatggc aaggtcatcg agcacgtcca cggcgacaag cagaacggcc acttcgaggc
     6841 cgagctgcaa ttcacgctga aaatcgacga gcccggctgg atcgccgtcc gcatcccgct
     6901 cgaagctggc cagaatgaat tcggcaagcc gcacttcgcc cacaccagcc cgatctacat
     6961 cgagctggca ggccgccgca tcttccgccc ggaagtcgcc cgtggcctga ttgccgacat
     7021 ggaaaagagc atcgaagcga tcaaggctga gggcaagttc gccgatgccg ccgaagaaga
     7081 ggcggtcctg agcgtctacc gcgagggcat cgcggtgatg cagcagcgat tgagtcaggc
     7141 ctcgaccggc cagtaacatt cacagctcaa tatccatgac gcccgatgac acatcagcta
     7201 ggatagaaat gatgaacgcc atgctgatgt cacttccgca gccgcccacg atggaggacg
     7261 tcttggaacg tctgggcggt gtttccccgt cgcgaattcg atttcaacca ttgccgggcg
     7321 aagccaccgt gcaggatgtt gtagagatcg aatcgcgaga gaatcgcctg tttgagctgg
     7381 tcgatggcgt cctcgtggag aaagtcatgg gcctgcgcga gtcgattctg gcggctgccc
     7441 tcgcggagtt gctgcgccga tttgtcgtgc cgcgaaacct gggcgtcgtc tcgggaccgg
     7501 acggcatgtt gcagctcttc ccggggttgg tgcgcatgcc cgacgtggcc tacgtatcgt
     7561 ggcagcggat tcccggcggc gagattcccg ccgaacccgc ccccttgctg gcccccgatt
     7621 tggccgtgga aattctcagc cgcagcaaca cccccgccga gatgagccgc aagctgggag
     7681 agtactttga ggccggcgtc agccaagtgt ggttcgtcga tctggattcc cgcaccatca
     7741 ccgtttatcg ctcgcccgat tcggctgacg tgcattctgt gccggcagtc atcgacggcg
     7801 gatcgctttt gcccgggttc tcgctgccat tggccgagtt attcgcggaa ctcgatcgcc
     7861 gcgggaactc gcctcctggt acgtaaagcg gcccacgtgc tccaacgacc aacgcgaggc
     7921 gcgcagacgt cgcagcttga cgacccggag gaggaagcgg tgctgagcgt cactcgcgaa
     7981 ggcatcgcgg cgatgcgcga gcgaattcag gaaaacgccc gatgagcatt cacagtaagc
     8041 gtgaggaacc tgcggatccc aacgcgattc gaccgctgtc accgacaatt cgaattcttg
     8101 tcattgtcgg cgaattgctg ctcgcatcct tgtttctccc attggtggcc ctctttccag
     8161 gcgtgctcgt tgaacgcgaa cctggtcctt tcgtcgtcat cggagtcggg tgtcttgtcg
     8221 ccgcatacgg attggggctt cagcgccgct ggatggttcc cgcaagtgtg gcgatggttg
     8281 cgatttcggg ttatacactg ctccgcgcgc ttgatgcgat tagcaagtcc gcagggtggg
     8341 gggtggtgct gctgacgatt gttcccct
//
LOCUS       DASUGB010000587         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1038786, whole genome shotgun sequence.
ACCESSION   DASUGB010000587 DASUGB010000000
VERSION     DASUGB010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038786"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VFV87_11270"
     CDS             <1..1693
                     /locus_tag="VFV87_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5104385.1"
                     /translation="SAWMEGEALDIQRLLYTPEGKPRIAILSIAHLTDAERMFFVTIV
                     LSEVISWMRSQPGTSSLRAILYMDEVFGYFPPSANPPSKTPMLTLLKQARAFGLGVVL
                     ATQNPVDLDYKGLANCGTWLLGRLQTERDKLRVLDGLEGAQAAAGAAFDRQAMERILS
                     GLGNRVFLMNNVHEDVPVVFQTRWALSYLRGPLTRDEISRLMAERKATVTRGVSEGTP
                     TRSVSEDVISRSELTTMSAEPRPIVAAMETQRPVLPPEIVERFASRRANLPPGAKVLY
                     KPALLGQAKMHYAQATAGVDAWQDVALLLAVAGPLPPELWATVEGELESPPDLEDQPE
                     AGAGFAELPGELSRPKRYSELATALKDYLYRNRKLSLWKCAALKQTSEPGETEAEFRI
                     RLGQLAREARDEQVENLRQKYGLKIAGLQERIRKAEMKVEKEKAQAQEKTWSAFLSAG
                     TSVLGALLGRKWASTANMTRAASAARAGSRAFRERQDIADATEDVKALQEQLAALEAT
                     AQAETTKVQDGLQPDNLELSEIQIAPKKSEINVSGVTLVWQPSIVGMDGVVKWGV"
     gene            complement(1690..2931)
                     /locus_tag="VFV87_11275"
     CDS             complement(1690..2931)
                     /locus_tag="VFV87_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5104386.1"
                     /translation="MQKIIVEKPYRFIPPYRHRFWPWVFRTVHLHAYYLWRSEGVWNY
                     EIRHLDRFRKSLAAGHAILLTPNHPRTADPLAMGWLAVEAPCHFWVMASWHLFQHSWL
                     YAWAIRAMGGFSVNREGVDRQSINMAVDLLAEAKRPLVIFPEGATSRTADRLQAMLDG
                     VAFIARAAAKKRAKLSPPGKVVVHPIAVKYYYSGDIRRAADDVLTDIEQRLTWQPQRS
                     LPLLERILKVGSALLSLKEIEYLNQPQTGPLSERLGKLTDRLLGPIETEWLGRVQSGP
                     VVPRVRNLRTKILPELVAGRLDGTERSRRWVQLADIYLAQQLACYPPDYLLRPTVDRV
                     LEMIEKFEEDLTDKARLHGHLRVVLDIGEAIEVSTGRDRSATIDPLMTRIQQSLQTMI
                     DELARESPVLERADSAPSTSA"
     gene            complement(3094..>4053)
                     /locus_tag="VFV87_11280"
     CDS             complement(3094..>4053)
                     /locus_tag="VFV87_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104387.1"
                     /translation="KDHTPAAGLIPYSVNAQLWSDHAIKERFIAIPGDAKIGFDEVTY
                     PQPSPGAPPGWKFPDGTVLVKTFSMETERGNPASAKRLETRLLHFQKFPGTEEYGDQY
                     WRGYTYVWNDEQTDAELLSADGADRVLKIKDGDKFVLQNYRFPSRAECTLCHTMGSKF
                     ALGVTTMQMNRDHNYGGTLANQLATLEHIGLFDKPLPKPPAELPKLADYDDESQSLDT
                     RARAYLHSNCAHCHIKWGGGNAEFKLVSTLPLAELGIVGTPPGHGRFNLDDPKLLVPG
                     QPDRSIILHRMQLTTLGRMPHIGSRVPHESAIKLIREWIAAQE"
BASE COUNT          720 a         1194 c         1414 g          725 t
ORIGIN      
        1 ctcggcgtgg atggaaggcg aggcgctcga catccagcgg ctgctctata caccggaggg
       61 aaagccgcgg attgcgatcc tctcgattgc ccacctgacc gacgccgagc ggatgttctt
      121 cgtcacgatc gtcttgagcg aagtcatttc gtggatgcgc agccagccgg gcacgtcgag
      181 cctgcgggcg attctgtata tggacgaggt gttcggctat tttccgccgt cggccaatcc
      241 accctcgaag acgccgatgc tcacgctgct caagcaggcg cgggcgtttg gtctgggcgt
      301 cgtgctggcg acgcagaacc cggtcgatct ggactacaag gggctggcga actgcggcac
      361 gtggctgctg gggcgattgc aaacggagcg cgacaagttg cgtgtgctgg acggcctgga
      421 aggggcgcag gccgcggcgg gggcggcttt cgaccggcaa gcgatggagc ggattttgtc
      481 gggtctgggc aaccgcgtgt tcctgatgaa caacgtacac gaggatgtgc cggtcgtgtt
      541 tcagacacgg tgggcgctct cgtatctgcg cgggccgctg acgcgggacg aaatcagccg
      601 gctgatggcg gagcggaagg cgacagtaac ccgaggcgta agcgagggca caccaacccg
      661 aagcgtaagc gaggacgtca tctcgcggag cgagttgact accatgagcg cggaaccgcg
      721 accaattgtc gcggctatgg agacgcagcg gccggtgctg ccgccggaaa ttgtggagcg
      781 gtttgcatcg cggcgggcca atctgccgcc gggggcgaag gtgctctata agccggcgct
      841 cctggggcag gcgaagatgc actacgccca ggcgacagcg ggcgtcgatg cgtggcagga
      901 tgtggcactg ctgctcgcgg tcgctggccc gctgccgccg gagttgtggg cgaccgtcga
      961 aggagagctg gaatcgccgc cggatttgga ggatcaaccc gaggccgggg cgggcttcgc
     1021 cgaattgccg ggcgagcttt cgcggccgaa gcgctacagc gaactggcca cggcgctcaa
     1081 ggactatctg tatcgcaacc gcaagctgag cctgtggaag tgtgcggcgc tcaaacaaac
     1141 gtccgagccg ggcgagacag aggcggagtt ccgcatccgc ctggggcagc tcgcccgcga
     1201 ggcccgcgac gagcaggtgg agaatctgcg gcagaagtac ggattgaaaa tcgcgggcct
     1261 tcaggagcga attcgtaaag cggaaatgaa agtggagaag gaaaaagccc aggcgcagga
     1321 gaaaacgtgg tcggcgttct tgtccgccgg gacttccgtc ctgggagcgc tgctcggccg
     1381 caaatgggcc agcacggcga acatgacccg cgccgccagt gccgcccggg ccggcagccg
     1441 ggccttccgc gagcggcagg acatcgccga cgccacggag gatgtgaaag ccttgcagga
     1501 gcagctcgcc gcactggaag ccacagctca agcggagacg acgaaagtcc aggacggctt
     1561 gcagcccgac aacctggagc tgagcgaaat ccagatcgcg cccaagaaga gcgagattaa
     1621 cgtcagcggc gtcaccctcg tctggcagcc gtcgatcgtg gggatggatg gggtcgtgaa
     1681 gtggggagtt taagcgctcg tcgatggcgc actgtcggcg cgttccagca ctggcgactc
     1741 gcgagcgagc tcgtcgatca tggtttgcag cgactgctgg atgcgggtca tgagcggatc
     1801 gatcgtcgcg ctgcggtcgc ggcccgtgct gacttcaatc gcttcgccga tgtcgaggac
     1861 gacgcggagg tggccgtgca gacgggcttt atcggtcagg tcttcctcga atttctcgat
     1921 catctcgagg acgcgatcga ccgtcggccg caggagataa tcgggcggat agcaggcgag
     1981 ctgctgggcg aggtaaatat cggcgagctg aacccagcgg cgcgagcgtt ctgtgccatc
     2041 caggcggcca gcgaccagct ccggcaggat tttcgttcgc agattgcgga ctcgcggcac
     2101 gaccggcccg gattgcacgc ggcccagcca ttccgtttcg atcggcccca gcagccgatc
     2161 ggtcagcttg cccaggcgct cgctgagcgg accggtctgc ggctggttga ggtattcgat
     2221 ttccttgaga ctgagcaggg ccgaaccgac cttgaggatc cgctccagaa gcggcaggct
     2281 gcgctgcggc tgccaggtga ggcgctgctc aatgtcggtg agcacgtcgt cggcagcccg
     2341 gcggatatcg ccgctgtaat agtacttgac ggcgatcgga tggacgacga ccttccccgg
     2401 cggcgaaagc ttggcccgtt tcttggccgc cgcccgggcg atgaaggcca cgccgtcgag
     2461 cattgcctgc aggcggtccg ccgtgcggct ggtggcgcct tcgggaaaaa tcaccagcgg
     2521 ccgcttcgcc tccgcgagca gatcgaccgc catgttgatc gactgccgat cgacgccttc
     2581 gcgattgacg ctgaagccgc ccatcgcgcg gatcgcccag gcgtacagcc aactgtgctg
     2641 aaacaggtgc cagctcgcca tcacccagaa atggcacggc gcttcgaccg ccagccagcc
     2701 catcgccagc ggatcggcgg tgcgggggtg gttgggcgtg agcaggatgg cgtgccccgc
     2761 ggcgagcgac ttgcggaacc ggtccaagtg gcggatctca tagttccaaa cgccctcgga
     2821 gcgccacagg taataggcgt gcaaatggac tgtgcgaaag acccacggcc agaatcgatg
     2881 ccgatacggc ggaatgaacc ggtagggctt ctcgacgatg atcttctgca taatgccgga
     2941 tcatggtatc cgattgccgc gctggtggca gcgacgatcc cacgcgatcg gcgctggttc
     3001 gtgtttgctc aagatcttta cagcgccgaa acatggtgcg gccgtggtcg aatcttgcca
     3061 ttaccgcgtt ttttggcacc acgaatcgtc agcctattct tgagctgcga tccactcgcg
     3121 gatcagcttg atggcacttt cgtgggggac gcggctgccg atgtggggca tgcggccgag
     3181 ggtggtgagc tgcatccggt ggaggatgat cgagcggtcg ggctgaccgg ggacaagcag
     3241 cttgggatcg tccaggttga aacggccgtg acccggcggc gtgccgacga tgcccaattc
     3301 cgccagcggc agcgtgctga cgagcttgaa ctcggcgttg ccgccgcccc atttgatatg
     3361 gcagtgggcg cagttgctgt gcagataagc tcgggcccgc gtgtcgaggc tttgcgactc
     3421 gtcgtcgtag tcggccagct tcggcagctc ggctggcggc ttggggagcg gcttatcgaa
     3481 caggccgatg tgctcgagtg tggcgagctg gttggcgagt gtgccgccgt aattgtgatc
     3541 gcgattcatc tgcatcgtcg tcacgcccag cgcgaacttc gagcccatcg tgtggcagag
     3601 cgtgcattcg gcccggctgg ggaagcggta gttttgcagg acgaatttgt cgccgtcttt
     3661 gatcttcagc acccggtcgg cgccgtccgc actcagcagt tctgcatccg tctgctcgtc
     3721 gttccagacg tacgtatagc cgcgccagta ctgatcgcca tattcctccg tgccagggaa
     3781 cttctgaaag tgcagcagcc gcgtctccag ccgcttggcg ctggccggat tgccgcgttc
     3841 cgtttccatc gaaaaggttt tgacgaggac ggtcccatcg ggaaacttcc agcccggcgg
     3901 cgcgccgggc gagggctgcg gataggtcac ctcgtcgaat ccgatcttgg catcgccggg
     3961 aatggcgatg aaccgctcct tgatcgcgtg gtcgctccaa agctgggcgt tgacgctgta
     4021 cggaatcagg ccggccgccg gcgtgtggtc ctt
//
LOCUS       DASUGB010000588         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1595374, whole genome shotgun sequence.
ACCESSION   DASUGB010000588 DASUGB010000000
VERSION     DASUGB010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595374"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /locus_tag="VFV87_11285"
     CDS             <1..3034
                     /locus_tag="VFV87_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104388.1"
                     /translation="DGTIQVQLTGVPTEVTEGALARFVSDIRPTGPLTLDANLTWAEG
                     GKTHHTVIKQLSAPKLSITAPELLTSETLSLAIQSAAADIEKASSKLAIRELRVDSSL
                     LQLSGRGSAETTSLAPSELIASLNSAVGTSEIELDGQIDLAELARQLPGTLRLREGTA
                     ITSGVVKASLASKSEQGQRRWQGSVKTADLRATNAGRPIAFDQPMEVSFNIRQTNNGP
                     VIDELTGQSSFASLRGSGTLTDGTITADADLDKLATEIGRLVDLGETRLAGTLAANLK
                     WNDDGRDAWKANADARIQRLEIQAAGLAPWREDELQLTAETTGALARSSLERLDAARL
                     TVASGADRLEVELTEPVKQFSAAAPWPAKFTLRGDLAAWAARLQPFVPLGSWRIGGTV
                     DASGAGHFAAQSSELAAVKVQVEQLAVDGPGLSIREPIVKIETAGAWDSQPQTLTLPM
                     LTLASSSVAFRADNLRVVASSQPSATGLIDFRGDLARLSSWLGDAQSPRTWLLAGAMT
                     GRVEIGYRGQSLEANWMTDIENLAYFTPPSDAAGRPPLAAVSSTTGWETRWTEPRVSA
                     SGQGTYDPATAKLQLSQANLISSMATLAAAGTITELTGACVLDLAGEVTYDLEPISER
                     LRASFAASPVSTLPGRPAAPRAIDSLQLVGQEKRPFILKGPLFASAALTPAAASLAGS
                     FSPPLPSAQPLVSDQLQGEASLGWQSAQFVGLVAGKADVRSRLDRGIVQVGPIDLPLS
                     EGRLSASPRVLLNDRVPAVVLDRGPLLENVRISPEMCQLWLKYVAPVLADVTRAEGKF
                     SLSLQGAAIPLAAPKQGDAAGNLAIQSAQVGPGPLAQQYLLLAQQIRTLFDPAAGAST
                     TIDPNRGWLTLPQQDVIFEVRQGAVHHRGFTMVVKDVAITTEGSVGIDDQSLNLIANV
                     PIQESWIKKADSSGLLMGLKGKTLQVPISGTMTQPKIDSRIVENLTKQLAGAAVQGAI
                     DQQLQKGQTILQDELGKGLNRLFGPLQPKPPAPMPPTP"
     gene            complement(3138..>4086)
                     /locus_tag="VFV87_11290"
     CDS             complement(3138..>4086)
                     /locus_tag="VFV87_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104389.1"
                     /translation="IELDLALADVQKFEQQTALLRRKLTAATGQQREQLAADLSTRQQ
                     QHPLNRLWTAPGPVTRQLLKARLAIFGTWCIPSICGTDELGRDLLSRICWGSRVSLIV
                     GIVATLVSLVIGVSYGAIAGYLGGSVDAAMMRVVDILYSIPFIFIVIYIITILSEESI
                     KNRLTKIGINQIFVFYVLIGAIYWLTMSRVVRGQIISLKHEQFVDAARTIGAGTGRIV
                     FRHLVPNVLGIVIVYLTLTIPSVMLFEAFLSFLGLGVQPPDVSWGLLVNDGVKIITPI
                     QQLWWLVLFPALALALTLFALNFLGDGLRDALDPRLKNR"
BASE COUNT          752 a         1356 c         1340 g          638 t
ORIGIN      
        1 cgatggcacg attcaagtgc agctcaccgg cgtgcccacc gaagtcaccg aaggagcgct
       61 ggcccggttt gtatccgata tccgccccac cggcccgctg acgctcgacg ccaaccttac
      121 gtgggccgaa ggcgggaaga cgcaccacac tgtcatcaag caactttccg ccccgaagct
      181 ctcaatcact gcccccgaat tgctgacgag cgaaacgcta tcgctggcga ttcagtccgc
      241 cgcggctgac atcgagaaag cctccagcaa gctggcgatt cgcgagctcc gtgtcgattc
      301 cagcctcctt cagctcagcg gccgtggatc ggcggagaca acgtcgcttg cgccctccga
      361 gctcattgcg tcgctgaact cggccgttgg cacgagcgaa atcgagttgg atggccagat
      421 cgacctggct gaactggccc gacagcttcc cggcacgctg cggctgcggg aagggacggc
      481 gattacgtcg ggcgtggtca aggcgtcgct ggcaagcaaa agcgagcaag gccagcgccg
      541 ctggcagggt tcggtgaaga cagccgacct gcgggccacc aatgccggcc gcccgatcgc
      601 cttcgatcag ccgatggagg ttagcttcaa catccgccag acaaacaatg gcccggtcat
      661 cgacgagctg accggccaat cgagcttcgc cagcctccgc ggatcgggga cgctcaccga
      721 cggcacgatc accgctgatg ccgatctcga caagctggcg acggaaattg gccggctggt
      781 cgatctgggc gagacccgcc tcgcgggcac gctggcggcc aatctgaagt ggaacgatga
      841 cggccgcgat gcctggaagg cgaacgccga tgcccgcatc cagcggctgg agattcaagc
      901 cgccggcctg gcgccgtggc gcgaggacga gctgcagctc acggccgaaa cgaccggcgc
      961 gctcgcccgg tcgagcctgg aacggctgga cgcggcccgc ctcacggtcg cctccggggc
     1021 cgatcgcctg gaggtcgagc tgaccgagcc agtcaaacag ttctcggcgg cagcgccttg
     1081 gccggcgaaa ttcacgctgc gcggcgattt agccgcgtgg gccgcacgcc tgcagccgtt
     1141 tgtgccgctg gggagttggc ggatcggcgg caccgtcgat gccagcggtg ccgggcactt
     1201 cgcggctcag tcgagtgagt tggccgcggt gaaagtccag gtcgagcagc tcgccgtcga
     1261 tggcccgggc ctgtcgatcc gcgagccgat cgtcaagatc gagaccgccg gcgcgtggga
     1321 cagccagccg caaacactca cgctgccgat gctcacgctc gccagctcgt cggtggcgtt
     1381 tcgcgcagac aatctgcggg tcgtcgcttc gtcgcagccg tctgcgaccg gactcatcga
     1441 cttccgcggc gacctggccc ggctgtcgtc gtggctgggc gatgcacaat cgccgcgaac
     1501 atggttgttg gccggggcga tgaccggccg cgtggagatc ggctatcgcg ggcaatcgct
     1561 cgaagccaac tggatgactg acatcgaaaa cctggcgtac ttcacgccgc catcggatgc
     1621 cgcgggaagg ccgcccctgg ccgcggtcag ctccactacg ggctgggaaa cgcgctggac
     1681 tgagccgcgc gtgagcgcga gcgggcaagg aacgtacgat ccggccactg cgaagctgca
     1741 gctatcgcag gcgaacctga tttcgtccat ggcgacgctc gcggctgctg gcacgatcac
     1801 agagcttacc ggagcgtgtg tcctcgatct cgcgggcgag gtcacctacg acctggaacc
     1861 gatctccgag cggctgcgag cgtcgttcgc tgcgtcgccc gtcagcacgc tgccgggcag
     1921 gccggctgcg ccgcgggcga tcgacagcct gcagctcgtc ggccaggaaa agcggccctt
     1981 catcctcaag gggccgctct tcgcgtcggc cgcgctcacg cctgccgccg catcgctcgc
     2041 cggcagcttc tcgccgccgc ttccgtccgc tcagccgctc gtttccgatc agcttcaagg
     2101 cgaagccagt ctcggctggc agagcgcgca gttcgtgggc ctggtggcgg ggaaggcgga
     2161 tgtgcggtcg cggctggacc gcggcattgt ccaggtcggt ccgatcgacc tgccgctcag
     2221 cgaaggacgg ctgagcgctt cgccgcgagt tctgctgaac gaccgcgtgc cggcggtggt
     2281 cttggaccgc gggccgctct tggaaaacgt ccgcatctcg cccgagatgt gccagctttg
     2341 gctgaaatac gttgcacccg tgctggcgga tgtgacgcgg gccgagggca agttctcgct
     2401 ctcgctgcaa ggggctgcga tcccgctcgc cgcgccgaaa cagggcgacg cagccggcaa
     2461 tttggcgatc caatccgccc aggttggccc ggggccgctc gcccagcaat acctgctgct
     2521 cgcccagcag atccgcacgc tcttcgatcc cgccgccggc gcctcgacaa cgatcgaccc
     2581 caatcgcggc tggctgacgc tgccgcagca agacgtgatc ttcgaagtcc gccagggcgc
     2641 ggtccaccac cgcggcttca cgatggtcgt caaggacgtg gcgatcacca ccgaagggag
     2701 cgtcggcatt gacgatcagt cgctcaacct catcgccaac gtgccgatcc aggaaagctg
     2761 gatcaaaaag gcggatagct ccggattgct gatgggcctg aaaggcaaaa cgctgcaagt
     2821 ccccatcagc ggcacgatga cgcagcccaa gatcgacagc cgcatcgtcg agaacctgac
     2881 caagcagctc gccggggccg cggtgcaagg cgcgattgat cagcagctcc agaaaggaca
     2941 gacgattctg caggacgaac tgggcaaagg cctgaaccgg ctgttcgggc ccctgcagcc
     3001 caagccgccg gctcccatgc cgccgacgcc ctgacctgtt tttagccggg agcaaacttg
     3061 ctcccgaagt agcgggaatg gactctccat tcgtgaaccg acggcacgcc gggaccaggt
     3121 ttggtcccgg ctaggaacta cctgttcttc agccgcgggt cgagggcgtc gcgcaggccg
     3181 tcgccgagga agttgagggc gaagagggtc agcgccaggg ccagggccgg aaacagcacc
     3241 agccaccaga gctgctggat cggcgtgatg atcttcacgc cgtcgttgac gagcagtccc
     3301 caggagacat ccggtggctg cacgccgagg cccaggaacg agaggaacgc ctcaaagagc
     3361 atcacgctcg gaatcgtcag cgtcagatac acgatcacga tgcccagcac gttcggcacc
     3421 agatggcgga acacgatccg gccggttccc gcgccaatgg tgcgggccgc atcgacgaac
     3481 tgctcgtgct tgagcgagat gatctgcccg cggacgacgc gcgacatcgt cagccagtag
     3541 atcgccccga tcagcacgta gaacacgaag atctggttga tgccgatttt cgtcagccga
     3601 ttcttgatcg actcttcgct gagaatcgtg atgatgtaaa tcacgatgaa gatgaacgga
     3661 atcgaataga ggatatcgac cacgcgcatc atcgccgcat cgaccgagcc gccgaggtag
     3721 cccgcaatcg cgccgtagct gacgccgatc accagcgaaa cgagcgtcgc gacgatgccg
     3781 acgatcagcg agacccgcga gccccagcag atccgcgata agaggtcgcg gcccagctcg
     3841 tcggtgccac aaatcgaggg gatgcaccag gtgccgaaga tcgccagccg ggccttgagc
     3901 aactgccgcg tcaccgggcc gggcgcggtc cacaagcggt tgagcgggtg ctgctgctgg
     3961 cgagtgctca gatcggctgc gagttgctcg cgctgctggc ccgtggccgc ggtcagcttg
     4021 cggcgcaaga gcgccgtttg ctgctcgaac ttctgcacgt cggcgagcgc caaatccagc
     4081 tcgatt
//
LOCUS       DASUGB010000589         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1595466, whole genome shotgun sequence.
ACCESSION   DASUGB010000589 DASUGB010000000
VERSION     DASUGB010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595466"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2228)
                     /locus_tag="VFV87_11295"
     CDS             complement(<1..2228)
                     /locus_tag="VFV87_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104390.1"
                     /translation="MAKEVLPPIAAEPQLSEVHAELAPLLSDMAAGLADTAARAKNAA
                     EVDSAVAQAREALTLANDARLVPGSLRPWQRLAEAEESLAVAEYSAKRSSAQDAALAA
                     IRKAAGGNPEGAYAARSELVLKYPDLAAAPALRDAFQEVAKAEAARVKPVELKGQAET
                     VEAVPEIVASMAFAAPLTTGSAPAPGRMFFAQAGSSVWALDGASGKLLWRRPSDRQPA
                     WVSPDAGSDVLVVDWLGRSLSRVKATTGALVWRRELDSPIAGSPLGTGADVFIATRAG
                     QIIKFDAASGNVANAAQLPQPVGVAPVAAGGRLFQLADHSHLYTLSAADLSCEGAIYL
                     GHAAVGVAVPPLVMDKHLVIAENSGAENSTLHVVALGDGGLPQGVVQRLDVSGLVLTP
                     PVVLGKHLAVLTDRGAPLTFEITKGGSTPLQRVGDAAAVLQDSLARYGLPIGSRLLVG
                     ENGLRLYAFDESSGNLTETWTAFADDTLLAPPQQAGEVLFCVRREPAGVGAIAAAVQA
                     TTGKPLWQTRIAVPLADGLMVVSSLGDAIGIDPEFTGSVVHEFPAPAEAQNTLPRITT
                     AIIAPGSGGILVPAGNSSQLFVLSKDASTKQRITLPGPRASPPIVWEGKILAACSSGD
                     VVLLNPADGRPMADPLVIPVRPGSRLDGGSLAAGESGPAVISDGRRTLRLVNLETTPR
                     RHLVQQTAAELAAPVASSVAVLGQHAFIVDQTGELRSFAIEGLRPGPTWPLGTGGIEF
                     GPA"
BASE COUNT          406 a          775 c          803 g          371 t
ORIGIN      
        1 gccggtccaa actcgatgcc gcccgtcccg agcggccagg tcggtccggg cctcaagcct
       61 tcgatggcaa acgaacgcag ttctcctgtc tgatcgacga tgaaggcgtg ctggccgagc
      121 acggccacgg acgaagcaac tggggccgcc agttcggcag cggtttgctg taccagatgt
      181 cgtcgcggag tggtttcgag gttgacgagt cgcagcgtcc ttcgcccgtc gctgatgacg
      241 gccgggccgg attctcccgc ggccagcgaa ccgccgtcca gccgcgagcc aggccgcacg
      301 ggaatcacca gcgggtcggc catcggacgt ccgtcagccg ggttcaacaa tacgacgtcg
      361 ccagaagaac aagcggcgag gatcttgcct tcccaaacga tcggcgggct ggcgcgcggg
      421 ccgggaaggg tgattctctg cttcgtggaa gcatccttgg aaaggacgaa tagctgactt
      481 gaattgccag cggggacgag gatgcctccg ctgccagggg cgattatggc agtcgtaatc
      541 cgcgggaggg tgttctgggc ttcggcagga gccgggaatt cgtgaacgac cgagcccgtg
      601 aactctggat caattccgat tgcgtcgccg agcgacgaaa ccaccatgag tccgtcagcc
      661 agcggcacgg cgatccgcgt ctgccagagc ggcttgccgg tggtggcttg gacggcggcg
      721 gcgatggcgc cgacgccggc cggctcgcgg cggacgcaga agagcacctc gcccgcctgc
      781 tgcggcgggg caagcagcgt atcgtcggca aatgcggtcc acgtctcggt gagattgccg
      841 ctcgattcgt cgaacgcata caaccgcagg ccgttttcgc cgacgagcag ccggctgccg
      901 atcggcaggc catagcgggc cagcgaatct tgcagcacgg cggcggcgtc gcctacgcgc
      961 tgcaggggcg tcgagccgcc cttggtaatc tcgaacgtga gcggtgcgcc gcgatcggtg
     1021 agtaccgcga ggtgcttccc cagcacgacg gggggcgtca gaaccaggcc ggagacgtcc
     1081 aatcgctgca cgacgccttg cggcaggccg ccatcgccta gcgccaccac atgcagcgtt
     1141 gaattctcgg cgccggagtt ctcggcgatc accaggtgct tgtccatgac gaggggcggc
     1201 acggcgactc cgaccgccgc gtggccaaga tagatcgctc cttcacacga caggtcggca
     1261 gccgacagcg tgtacaggtg cgagtggtcc gccagttgaa acagcctgcc gccagccgcg
     1321 accggcgcga ctcccactgg ctgcgggagt tgggccgcat tggccacatt gccggaagcg
     1381 gcatcgaatt tgatgatttg acctgctcgc gtggcgatga aaacatccgc gccggttccc
     1441 agcggcgagc cggcaatcgg gctgtccaac tcgcgccgcc ataccagcgc tcctgttgtc
     1501 gccttcacgc gcgacaacga ccggcccaac caatcaacga ccagcacatc gcttcccgcg
     1561 tcgggcgaga cccaagccgg ctgccggtcg gagggccgcc gccaaagcag cttgccgctg
     1621 gccccatcga gggcccaaac cgaagagcca gcttgcgcga agaacattcg gcctggagcg
     1681 ggggcgctgc ccgtcgtcag tggagcggcg aacgccatgc tggcgacgat ttcgggcacc
     1741 gcttcgaccg tctcggcctg gcctttcagt tcaaccggtt tcacgcgggc cgcttcggcc
     1801 ttggcgacct cttgaaaagc atcgcgcagc gcaggagccg cggccagatc gggatacttc
     1861 agcactagct cgctgcgcgc ggcatacgcg ccctcgggat tgccgccagc agccttccgg
     1921 atagcggcga gggctgcgtc ctgtgccgag gagcgcttcg ccgagtattc ggcgactgcc
     1981 agcgattcct ccgcctcggc caaacgctgc cagggccgta gcgagccggg gacaagccgg
     2041 gcatcgttgg ccagcgtcaa ggcctcgcga gcctgagcga ctgccgagtc aacttccgct
     2101 gcgttcttcg ctctcgccgc cgtatcggcc aagcccgcgg ccatgtccga gagtaacgga
     2161 gccagttcgg cgtgaacctc cgaaagttgc ggctcggcgg cgatgggcgg gagaacttcc
     2221 ttggccaccg gcaggatctt cggccaatcg gtcggactgg aagaagccgt gtgcaggcgg
     2281 gccatcgcgc ggcggacttt tgccggcggc gcgagagggc tggagggaaa cctcgcgagc
     2341 gcggcatcgt acttc
//
LOCUS       DASUGB010000590         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1316386, whole genome shotgun sequence.
ACCESSION   DASUGB010000590 DASUGB010000000
VERSION     DASUGB010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316386"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFV87_11300"
     CDS             complement(<1..368)
                     /locus_tag="VFV87_11300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696665.1"
                     /note="catalyzes the decarboxylation of diaminopimelate
                     into lysine; pyridoxal phosphate(PLP)-binding; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5104391.1"
                     /translation="MPTAEPYSPVRTQIAGVKIADLASRFGTPTYVYDAAKIVERIND
                     LKQFDVIRYAQKACSNLAILDLVRRNGVVVDAVSAGEVRRALAAGYAVKGTGNRGQGT
                     GGHPEIVYTADIFDRESLDLV"
     gene            497..1180
                     /locus_tag="VFV87_11305"
     CDS             497..1180
                     /locus_tag="VFV87_11305"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5104392.1"
                     /translation="MNKTFKWIGAVLALIAMLVFVVPFALPSRYRVVRTVTINAPPAA
                     IHPSVNTLKEWPKWSAWTTERYPDMKITYFGPESGVGAIDEFEGDSLGVHGKFEITKS
                     DPDEGIEYRLDFDDGNYVSTGGIKYTPQGAGTNVTWSNEGELGMNPISRWCNALGMMD
                     KMMGPDFETGLANLKKKVEAEAAEQPGGEQPAGDTSAGEQSTDETPSTEESAKEPAVE
                     QPSAVTTEP"
     gene            1310..2395
                     /locus_tag="VFV87_11310"
     CDS             1310..2395
                     /locus_tag="VFV87_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5104393.1"
                     /translation="MHRCTSIAAAISLCLPLAARAADHPAPIVIADSAAQTEAEMKPY
                     TELIEHTDARIDMLPIKGGRLLMGSPEDEKGRYSDEGPQHEVELSPFWMARCEITWDA
                     YEVWMADLDIIKRQVLGIPETPRDKLAEIFQISRPTKPYTDMTFNMGKRGYPAICMTQ
                     HSCRVFCQWLSAKTGRYYRLPTEAEWEYACRAGTTTAYNFGDDPAELSEHAWWLDNSG
                     EKYQKVGKKPPNAWGLCDMHGNVSEWVLDQYTTDFYAKCKAQGVVKDPLAIPLTEYNR
                     VTRGGSWDDGPRRNRSASRVPSSDDWKQQDPQVPQSIWYFTDATFLGFRIVRPLVEPS
                     EEEKAAKWDKSEPPQIDKKELPVEGKE"
     gene            2395..3042
                     /locus_tag="VFV87_11315"
     CDS             2395..3042
                     /locus_tag="VFV87_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104394.1"
                     /translation="MPKFYCNDWADRTNPWAMTNPYQSPAFDPKQFQDSPFQAPVSAN
                     DYGWVSQVRIVAILNGVQGGLEIPMGLLYVGMAFFLPAMMQMGAANQPPGGSAPPKEF
                     FWFMTGLYLVMGIPVLLAGILRILAAWNNFHFRGRTLGILSMVIGMASAFSCYCAPTG
                     IALLVYGLVVYLNPAVRLAFDMGSQGLAASQILGSFVPYRGGPPAPAANGSPPQG"
     gene            complement(3136..3261)
                     /locus_tag="VFV87_11320"
     CDS             complement(3136..3261)
                     /locus_tag="VFV87_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104395.1"
                     /translation="MPIEFLDPLGLCPQAQQAFENLKIALEWEISSPFRLGSQHA"
BASE COUNT          908 a         1388 c         1493 g          776 t
ORIGIN      
        1 accagatcga gcgactcgcg gtcgaagatg tcagccgtat agacgatctc cggatgcccc
       61 cctgtcccct gtcctctgtt ccctgtcccc ttaaccgcat accccgccgc cagcgcccgc
      121 cgcacctccc ccgcgctcac cgcatcgacc acgaccccat tcctacgcac cagatcgagg
      181 atcgcaagat tcgagcacgc cttctgcgcg tagcgaatca cgtcgaactg cttcaggtcg
      241 ttgatccgct ccacgatctt cgccgcgtca tagacgtacg tcggcgtgcc aaaccggctg
      301 gccaggtcgg cgatcttgac tccggcaatt tgcgtgcgaa cgggcgaata cggctcagcg
      361 gtgggcatgg cagggaggag ggcgttttgc gtggaaggtt cgttctcggt tagcataatg
      421 cctaccccat ccggcggtag gggctcggcc cgccgagttc gcactccatt tcttcctgca
      481 accgtcgtcc tgctatatga acaagacctt caaatggatc ggcgcggtgc tcgcgctgat
      541 cgccatgctg gtgttcgtcg ttccgtttgc gctgccgagc cggtaccgcg ttgtgcggac
      601 ggtgacaatc aacgccccgc cagcggcgat ccatccttcg gtcaacacgc tcaaggagtg
      661 gcccaagtgg agcgcctgga cgacggagcg ctatccggac atgaagatca cctattttgg
      721 gccggaatcg ggcgtcggcg cgatcgatga gtttgagggg gattcgctcg gcgtccacgg
      781 caagttcgag attaccaaga gcgatcccga tgaggggatc gagtacaggc tcgattttga
      841 cgatggcaac tacgtttcga ccggcggcat caaatacacg ccgcagggcg ccggcaccaa
      901 cgtgacttgg tcgaacgaag gcgagctcgg catgaatccc atcagccgct ggtgcaacgc
      961 cctgggcatg atggacaaga tgatgggccc cgattttgaa acgggtcttg cgaacctcaa
     1021 gaagaaggtc gaagccgaag ccgcggagca gccgggtgga gagcagccag ccggcgacac
     1081 atcggccggc gagcaatcaa ccgatgagac gccgtccacg gaggagtcgg ctaaagagcc
     1141 ggctgtcgag caacccagcg ccgtgacgac cgagccgtga ggcaaggcgc ggcggaaagt
     1201 ccggcgtccg cgtccttgaa ccgagcatgc cggtactgag tgctccgcac tgagtacaat
     1261 gagggacgcg ccaccgaccg acgcccgcca tttacgggac agcccgccca tgcaccgatg
     1321 cacttccatc gccgccgcga tctcgctctg cctgccactc gccgcacgag ccgccgacca
     1381 ccccgcgccg atcgtcattg cggattcggc ggcccagacc gaagcggaaa tgaagccgta
     1441 cacggagctg atcgagcaca ccgacgcccg gatcgacatg ttgccgatca aaggggggcg
     1501 gttgctgatg ggtagccccg aggacgaaaa gggccgctat tccgatgagg ggccgcagca
     1561 cgaggtcgag ctgtcgccct tctggatggc ccgctgcgag atcacgtggg acgcgtacga
     1621 ggtctggatg gcggacctcg acatcatcaa gcggcaggtg ctcggcatcc ccgagacgcc
     1681 gcgcgacaag ctggccgaaa tcttccagat cagccggccc accaagccgt acaccgacat
     1741 gacgttcaac atgggtaaac gcggataccc ggcgatctgc atgacgcagc attcgtgccg
     1801 tgttttttgc cagtggctga gcgccaagac cggccgctac taccgcctgc cgaccgaagc
     1861 ggagtgggaa tatgcctgcc gggccgggac gacgacggcg tacaactttg gcgacgaccc
     1921 ggccgagctg agcgagcacg cctggtggct cgataacagc ggcgagaagt atcagaaagt
     1981 cggcaagaag ccgccgaacg cctggggcct ttgcgacatg cacggcaacg tgtcggaatg
     2041 ggtcctcgac cagtacacga ctgactttta cgccaagtgc aaggcgcagg gcgtggtgaa
     2101 ggatccgctg gcgattccgc tgaccgagta caaccgcgtc actcgtggcg gctcgtggga
     2161 cgacggcccg cgccgcaacc gcagcgcctc gcgcgtcccc tcgtccgacg attggaagca
     2221 gcaagacccg caggtgccgc agagcatctg gtacttcacc gatgccacgt tcctcggctt
     2281 ccggatcgtc cgcccgctgg tcgagccctc cgaggaagaa aaagccgcca aatgggacaa
     2341 atccgagccg ccgcagattg ataagaagga gttaccggtg gaaggaaagg agtagtgccc
     2401 aagttctact gcaatgactg ggctgatcgc acgaatccct gggcaatgac caacccctac
     2461 caatcgccgg ctttcgatcc caagcagttt caggattcgc ccttccaggc gccggtttcg
     2521 gccaacgact atggctgggt cagccaggtg cggatcgtgg cgattctcaa cggcgtacaa
     2581 ggaggcctgg agattcccat gggcctgctc tacgtcggga tggcgttctt ccttccggcg
     2641 atgatgcaga tgggcgccgc caatcagccg ccgggcggaa gcgcgccgcc gaaggagttt
     2701 ttctggttca tgaccggcct ctacctggtg atgggcattc cggtgctgct ggcgggcatc
     2761 ctgcggatct tggcggcgtg gaacaacttt cacttccgcg ggcggacgct gggcatcctc
     2821 tcgatggtga tcgggatggc ctcggcgttt agctgttact gtgcgccgac cggaatcgcc
     2881 ctcctggtgt acggcctggt cgtgtatctg aatccggccg tcaggctggc tttcgacatg
     2941 ggctcgcaag gccttgccgc cagccagatt ctgggcagct tcgttcccta tcgcggcggc
     3001 cccccggctc ccgctgccaa cgggtcgccg ccgcaggggt gaggtcgtca aaatcagcga
     3061 caagagccga atccgtgcct ttcgcgcacg ctggagtcag gatacaggca tcggctctgc
     3121 gggacgcaga cctcatcaag catgctgcga tcccaatctg aagggactcg atatctccca
     3181 ttccagggcg attttgaggt tctcgaacgc ctgctgggcc tgcgggcaga ggccgagcgg
     3241 atcgagaaac tcgatcggca tggcgatggc tcccgattgc gcgcgtgaat cctgaaacaa
     3301 tccgcgcacg ataaccgctg gcgtgcgagc gcggaagcaa ttgggtgggc gtcacgtttg
     3361 caggcggcgc gagcgccaag gggtcgggag tcgattgcgg cgacctactt tccatccgca
     3421 cgacggtgtc cgcaatcgac tcccgacccc ctcgaggttg gcgtgcgtca ccgccgggcg
     3481 ccgcctgccg atgccgtccg aacttgtaca cgccggattg gacgcacccc gaatggacca
     3541 gacgtgtcct tttcacggag gcaggcgaaa aaacttaggg cgagaggcgg cgacgtaagc
     3601 ggtgtgtgta caggagttta tgcgcgggcg cttcgccgta agtccacgca ggacaagaaa
     3661 gtcggcagaa tttgcttggg gcgcagggaa gcggtatgta agctcccgga caatcgtgta
     3721 cgaattatta gaatcgcggg aaagtgcatc gccggcggcc aggatggggt cgggagtcgt
     3781 ttttcggcca acccgctaat agaccgcgaa agaccgctgg ccgaaaaaga cgctcgcccc
     3841 ccctgtgaca tgcaacacca tccagcgcgg tgcctgcgct cgctggtccc actctgccaa
     3901 tttcctcgct gacgcgtcgg ggtgatgagg cggggggaag cgcggtgcga ggcgctgcgg
     3961 cgaaatgttc gaggctgagg gggtaagggt cgaaagggaa cgacgctgcg cgtaaccgat
     4021 cgaaaacagc gcaacagcgc aacatcctcg tgtggggggt gttgcgcggc gcaagtggct
     4081 gttgcgatag gggttaaata cgatgttgcg cggggcgagc ggcctgaaaa atgactggaa
     4141 gttgcgcgct gcgcccaacc tgcgcggggg tggagtcgtg gagccaagac aggaggcggg
     4201 agcctccagg agggcgttcc caggcagagc ctcggaacga gatcaatgga aggccagcgc
     4261 gcaaccgctt agcacagcgc aacagcgcaa catcctcaag cggggggtat tgcgcggcac
     4321 aagtggctgg tgcgatagga gttaagacaa tgttgcgcgg gttggatcgg cgggaagaaa
     4381 acttggaaag ttgcgcgctg cgccacgagc ctgcgagcgg ccacccgagt tgtggagcca
     4441 agacaggagg caggagcctc cacgaaggac gttcccaggc agagcctggg aacgagagcc
     4501 cgcactctgg cgagtgcggc tacgtttgga aacagcgttt agtagccgcg ggccagcgcg
     4561 gcgcg
//
LOCUS       DASUGB010000591         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_761290, whole genome shotgun sequence.
ACCESSION   DASUGB010000591 DASUGB010000000
VERSION     DASUGB010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761290"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFV87_11325"
     CDS             <1..305
                     /locus_tag="VFV87_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5104396.1"
                     /translation="KELLDLVGLGHRLTHRPRELSGGEMQRAAIARALVAQPKLLLAD
                     EPTGNLDQKTGGEILALLQKLNRQQGLTIVMVTHDQSIARRADRTVPLAGGKVAAA"
     gene            1225..1752
                     /locus_tag="VFV87_11330"
     CDS             1225..1752
                     /locus_tag="VFV87_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104397.1"
                     /translation="MSSETQPPRPSPWRFSIREIMLLTAAVAGFLAWANLAYQRSRPY
                     RRTPIPDTVGRFDDIQRICTEIGHRASSLSSGGGGSGDPYTTTRTYECRIDLPKPLRP
                     EFMKRYRERLRAMLDKHAENVSGAGTTSDGTGLRGFEYDYRQGRARGTVIVRTSGSDE
                     DFFLFLFIHEFDGRD"
     gene            1964..>2745
                     /gene="ilvE"
                     /locus_tag="VFV87_11335"
     CDS             1964..>2745
                     /gene="ilvE"
                     /locus_tag="VFV87_11335"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEX5104398.1"
                     /translation="MSLQIYINGKYYDKENAKVSVYDHGFLYGDGVFEGMRSYGGKVF
                     RLQQHLERLWNSAKAIWLKMPISQEEMAKAVTGTLAKNNIQDGYIRLVVTRGAGALGL
                     DPNRCSDPQVIVIADHIQLYPKEMYANGLEIITASTIRNHSAALSPRIKSLNYLNNIM
                     AKIEGLQAGCVEALMLNAKGEVAECTGDNIFIVRGGTLITPPIDAGILEGITRDAVIE
                     IAGELKIPVQQIALCRHDIFIADECFLTGSAAEIIPVVKLDSR"
BASE COUNT          504 a          996 c          761 g          484 t
ORIGIN      
        1 ccaaggagtt gctcgacctg gtcggtctcg gccaccgcct gacgcaccgc ccgcgcgagc
       61 tttccggcgg cgaaatgcag cgcgccgcca tcgcccgcgc cttggtcgcc cagcccaagc
      121 tcctcttggc cgacgagccc accggcaacc tcgaccagaa gaccggcggc gaaatcctgg
      181 ccctgctcca aaagctcaac cgccagcagg gcctcacgat cgtcatggtc acgcacgacc
      241 agtccatcgc ccgccgggcc gaccgcaccg tcccgctggc cggcggcaaa gtcgccgctg
      301 cataggcccc gccgacgtgg ggcaggcttt ccagcctgcc gtcttcgtgg ggcaggcttt
      361 ccagcctgcc actctctggc tcgtcgcctc ccgcgcacct ccgtcccttg gctccgcctg
      421 ggacggaacc tgcccgatgc tccgcctcgc gaactgggcg caactttcca gcttttttcc
      481 aagcgcaacc tcccgcgcaa cttcctccta acccacatcc gtgcaaccac ttgcgctgcg
      541 caacaccccc ctgatgagga tgttgcgctg ttgcgctgca cctccgtccc aggctcaacc
      601 tgggccggaa cttctccgag ggtccgcccc acgatccacc gagcgcaggc gcgcaacttt
      661 ccagcttttc ttcaagcgca acctcccgcg caacttcctc ctaacccaca tccgtgcaac
      721 cacttgcgct gcgcaacacc cccctgatga ggatgttgcg ctgttgcgct cctgtgatcg
      781 ttctccgcgc aacgcaaatt tcgccgggac gctcgcaccg caatccgctt gggaccctct
      841 ggtcccggcg tgtcgcgcct ccatgatttg tgcatcgcga gcattggaat tgcccgtccg
      901 atttcgtagg gtgggctgtg cccaccatcg cgtacggtat gtggaattcg tgggcagaac
      961 ccatccacgc acgattggtc gggagtttac aaactgcctc cctgcgcccc aagcaaattc
     1021 ggccgacttt cttggccagc gcgaacttgc gtcgatcccc cgaatcataa actcatgtac
     1081 agaatgtgtt tgcgccgtag tctctcgccc taagttttct tgaaccgccc gcgcgaaaag
     1141 gacacgtctg gtccatttcg gcacgaccga ttcacgcccc ttcgccgcga tacaatcgag
     1201 agcctcccaa ataccgaggt gtggatgtcc agcgaaaccc agccgccgcg gccatcgccc
     1261 tggcggttct cgatccgcga aatcatgctg ctgacggcag cggtggccgg gtttctggcg
     1321 tgggccaatc tcgcgtacca gcgcagccgg ccctatcggc ggacgccgat acccgacacg
     1381 gtcggccggt tcgacgatat tcagcggatc tgcacggaaa tcgggcatcg ggcctcgtcg
     1441 ctctcgtcag gcgggggcgg ctcgggcgat ccttacacga cgacccggac ctacgaatgt
     1501 cgcatcgacc tgccgaaacc cttacggccg gaattcatga agcgctaccg cgagcgcttg
     1561 cgggccatgc tcgacaagca cgcggagaac gtctccggcg ccggaacgac cagcgacggc
     1621 acgggcctgc ggggcttcga gtacgactac cgccaaggcc gggcccgcgg caccgtcatc
     1681 gtgcgcacct cgggcagcga cgaagatttc ttcctgttcc tgttcatcca cgagttcgac
     1741 ggccgggatt gaccaccgcg gcgcgaacct cccgccttgg cccgccgtcc tcccgcccgc
     1801 gggccttttc cgccgctgcc aaggccgctt ttggaaggta gaattgtgac cgtgacccgc
     1861 agcggcaccc atccaaagga gtcttcccat gccgagcgtc gcacctgccc ctccccagtc
     1921 tgcttcggcc ggccgcccgg ccccctcttc cgcccagccg aacatgtcct tgcaaatcta
     1981 catcaacggc aagtactacg acaaagagaa cgccaaggtc agcgtctatg accacggctt
     2041 tttgtacggc gacggcgtct tcgaggggat gcggagctac ggcggcaagg ttttccgcct
     2101 gcagcagcac ctcgagcggc tgtggaattc ggccaaggcg atctggctca agatgccgat
     2161 cagccaggaa gaaatggcca aagcggtgac tggcacgctc gccaagaaca acatccagga
     2221 cggctatatc cgcctggtcg tcacccgcgg cgccggggcc ctgggcctcg atcccaaccg
     2281 ctgcagcgat ccgcaggtga tcgtcatcgc cgaccacatc cagctctatc cgaaagagat
     2341 gtacgccaac gggctggaga tcatcaccgc cagcacgatc cgcaaccact cggctgcgct
     2401 cagcccacgg atcaagtcgc tgaactatct gaacaacatc atggccaaga tcgagggctt
     2461 gcaggccggc tgcgtggaag ccctaatgct caacgccaag ggcgaagtgg ccgagtgtac
     2521 cggcgacaac attttcatcg tccgcggcgg gacgttgatc acaccgccga tcgacgcggg
     2581 aattctcgaa ggcatcaccc gcgacgcagt gattgaaatc gctggcgaac tgaagatccc
     2641 cgtccagcag atcgccctct gccgccacga catcttcatc gccgacgaat gcttcctcac
     2701 cggcagcgcc gccgagatca tccccgtcgt caagctcgac agccg
//
LOCUS       DASUGB010000592         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_206206, whole genome shotgun sequence.
ACCESSION   DASUGB010000592 DASUGB010000000
VERSION     DASUGB010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206206"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2284
                     /locus_tag="VFV87_11340"
     CDS             <1..>2284
                     /locus_tag="VFV87_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104399.1"
                     /translation="GRLVEGQIFSEGTIDYVRITRGAVEPASSPSAEVTDATLGLWNF
                     DQQAAEAADLSPLKNAAKADLAAKAISMPPPQAPMPPPGVHLKPLDPTLKAVLIDRSE
                     NDVFMAVKCDSMGRLFVGGREAVFVFEPNDKGGYQPKQELMHFPQDSIIIGLEMRGND
                     LYVLTNSALYLLPEARVKRENLTCRRLLWGLPLNLHVSFHCLAWGPEGDLYLNHGDPM
                     LGYGDWSRPDAWSHWTYILADGTRVPYTGTGAVLRIKPEGTDLKVVATGLRGPVGLCF
                     DPDWNLFTNDNDHESQADRYSPARLLHVTPHIDFGWPRGWMASKSPERSDLIDAMQDD
                     HGRGVPCDMTWYREPLIPQLNNKLLLCRWERFSVTANTLKPSGATFRAVEEVIATGDH
                     NARPSGICVGRDGRIFVTSLYLPGNVTSPYCPSDLVMVTHIEDTEAHPFHPYDETKLS
                     KEELAKEGAKISWERRQHAQRELLRRGETPPGRATIQSLWELGEGFDQPKSLTDEQAV
                     GIAAMTAGPDPHALQVASRLLAEKGELAHLKYLGAQPESRLRLVGVLAIGMRLTTPPR
                     HGLLPPYLPLHYEADGAFFKRNVPYVGEPEPIDLSTIGRIGSFTTAEWWRAIERTPEQ
                     EELFALLVKALGDEDPRVQSQAAYYLSLLKDERSEPKVEAARRAALAKELSQRPELQI
                     GRVWLAGPFAAPPGEKVNPPELGAIDLTSEYPAEGGIIRWRELAAAEGKLPVPAAAGA
                     SANYLYFRLQSAARQPARLKI"
BASE COUNT          443 a          718 c          730 g          393 t
ORIGIN      
        1 gggcggcttg tcgaagggca aatcttcagc gaaggaacga ttgactatgt ccgcatcacg
       61 cgcggcgcgg tcgagcccgc ctcgtcaccg tccgccgaag tgactgacgc cacgctcggc
      121 ctctggaatt tcgatcaaca agccgccgaa gccgccgacc tttcgccgct gaagaacgcc
      181 gccaaagccg atctggccgc caaagccatc tcgatgccgc ccccgcaggc tcctatgccg
      241 ccgccgggcg tgcatctgaa gccgcttgat ccgacgctga aggcggtgct gatcgaccgt
      301 tcggaaaacg acgtcttcat ggcggtcaag tgcgacagca tgggccggct gttcgtcggc
      361 ggtcgcgagg cggtcttcgt ctttgagccg aacgacaaag gaggctacca gccgaagcag
      421 gagctaatgc acttcccgca ggactcgatc atcatcggcc tggagatgcg gggcaacgat
      481 ctgtacgtgc tcaccaactc cgcgctctac ttgctgccgg aagctcgcgt gaagcgcgag
      541 aacctgacct gccggcgact gctctggggc ctgccgctca atctgcacgt cagcttccac
      601 tgcctggcgt gggggccgga gggagacctg tacctcaacc acggcgatcc gatgctcggc
      661 tatggcgact ggagccggcc ggatgcctgg agccactgga cgtacatcct ggccgacggc
      721 acgcgcgtgc catacaccgg caccggcgcg gtcctccgca tcaagcccga aggcacggac
      781 ctgaaggtgg tcgccaccgg cctccgcggg ccggtcggac tgtgcttcga tcctgattgg
      841 aacctgttca ccaacgacaa cgatcacgaa agccaggccg accgctattc gccggcccgg
      901 ctgctgcacg tcacgccgca catcgatttc ggttggcccc gcggctggat ggcttccaaa
      961 tcgccggagc gctccgacct gatcgatgcg atgcaagacg accacggccg cggcgtcccc
     1021 tgcgacatga cgtggtaccg ggagccgctg atcccgcagc tcaataacaa actgctcctc
     1081 tgccgctggg agcggttttc agtcacagcc aatactttga agccctctgg cgcgacgttt
     1141 cgggcggttg aggaagtaat tgccaccggt gatcacaacg cccgccccag cggcatctgc
     1201 gtcggccgcg atgggcggat ttttgtcact agcctctatc tgccgggcaa cgtaacttcg
     1261 ccgtattgcc ccagcgacct ggtaatggtt actcacatcg aggacaccga agcgcatccg
     1321 tttcatcctt acgacgaaac gaagctgtca aaggaagaat tggcgaagga aggagccaag
     1381 atctcttggg aacgtcggca gcacgcgcaa cgagagttac tgcgacgagg tgagacgcct
     1441 cctggccgag cgacaatcca gtcgttgtgg gagcttggtg agggattcga tcaacctaag
     1501 tcgctaaccg atgaacaagc tgtggggatt gcagcaatga ccgccggacc cgatccgcat
     1561 gcattgcaag ttgcgtcaag gcttttggca gagaagggag aacttgctca tctgaagtat
     1621 cttggcgcgc agccggaaag ccgtcttcga ctggtgggag tgttggccat cggcatgcgg
     1681 ctgacaacac cgccgcggca tgggttgttg ccgccatacc tgccgctgca ctacgaggcg
     1741 gacggggcgt tcttcaagcg gaatgtgccg tatgtcggcg agccggagcc gatcgatctg
     1801 agcacgatcg gccggattgg cagctttacg accgccgagt ggtggcgggc gatcgagcgg
     1861 acgccggagc aggaagagtt gttcgcgctg ctcgtcaagg cgctgggcga cgaagacccg
     1921 cgcgtgcaat ctcaggccgc ctattacctg tcgctgctga aggacgaacg gagcgagccg
     1981 aaagtcgagg cggcgaggcg cgcggcactg gccaaggaac tgtcgcaacg accggaattg
     2041 caaatcggac gagtctggct ggcggggcca tttgccgcgc cgcctggcga gaaggtgaat
     2101 ccgcccgaac tcggcgcgat cgacctgacg agcgaatatc ccgcggaagg cggcatcatc
     2161 cgctggcggg agcttgccgc tgcggagggc aagctgccgg ttccagcagc cgctggcgcg
     2221 agcgcgaact atctctattt ccgccttcaa tccgccgccc ggcagccggc ccggctgaag
     2281 atcg
//
LOCUS       DASUGB010000593         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2012166, whole genome shotgun sequence.
ACCESSION   DASUGB010000593 DASUGB010000000
VERSION     DASUGB010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012166"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VFV87_11345"
     CDS             <1..1303
                     /locus_tag="VFV87_11345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5104400.1"
                     /translation="IVTELGTRVDPLRQEIRVDGQPLRRPQRLYFAVNKPVGVVTTNN
                     DPSGRPRVIDLVPTDERVFAVGRLDRASEGLILVTNDGEFANRITHPRYGVEKTYLVR
                     VAGSPTPADLARLTKGVYLSDGFCRVQSITVKRKQGQSTDLVMVLNEGRNRELRRILA
                     RVGHKVLRLKRIAVGSIKLADQPVGAWRRLAPDEIESLLRLAKEKRKAAKSKRKTPRL
                     ADGQQPGGMKPVGGQRQESPYRQQQALMAEPLSLADLLRDDLDEGNLAAEEVADTGGI
                     PASAAEQESYEDAELRFSKESQRGKPGGVIAYEGGEASGAEPRGRRGGKRRRRDAHGR
                     IAAEQQPEKLPRESFGKKRSGKRIFGRPHQGRGPAGQTAGNFDYRPAGQPKKKSIQKD
                     ERPSRPAKRGKQRFAKAAGGKFGKPGGGKKFVGRSKRKGRR"
     gene            1300..>2103
                     /locus_tag="VFV87_11350"
     CDS             1300..>2103
                     /locus_tag="VFV87_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5104401.1"
                     /translation="MNLQASPLHARFCPDQAVQLPAVIVENVRLARDTFRVRLHAPEI
                     ARRIVPGQFVMARIAGLNDPLIGRPLALYDTVIGTDGQPAAIDLVYLVKGKLTTRLCG
                     MQPGQLLDVWGPLGNGFGAEGSGAGDQGAEGGVQGSGIRVQGPEIGARTSDAGEGVPR
                     SIHHRLAAVGLRGKRLHAARQHLILVAGGIGQTPFLALAQEALHLKRYGEPPREVPRA
                     ERITFCYGARTADYLAGVEDFERLGIDVRIATDDGSRGHPGLVTELLEQV"
BASE COUNT          396 a          663 c          744 g          300 t
ORIGIN      
        1 gatagtgacg gaactgggca cgcgcgtcga tccgctgcgg caggagattc gcgtcgatgg
       61 ccagccgctg cgccggccgc agcggctgta tttcgccgtc aacaaaccgg tcggcgtggt
      121 cacgaccaac aatgacccca gcggccgccc gcgagtgatc gacctggtgc cgaccgacga
      181 gcgggtcttc gccgtgggcc gcctcgaccg ggccagcgag gggctgatcc tggtaaccaa
      241 cgatggggag ttcgccaacc ggatcacgca cccgcgctac ggcgtggaaa agacctacct
      301 ggtccgtgtc gcgggctctc ccacgcctgc cgacctggcg cgactcacga agggcgtgta
      361 tctgagcgac ggcttttgcc gcgtgcagtc gatcaccgtc aagcgcaagc aaggacaaag
      421 caccgacctg gtgatggtcc tcaacgaagg ccgcaatcgc gagttgcggc gaatcctggc
      481 ccgcgtcggc cacaaggtat tgcgactcaa gcggatcgcg gtcggttcga tcaagctggc
      541 cgaccagccg gtgggggcct ggcggcggct ggcgccggac gaaatcgagt cgctgcttcg
      601 cctggcgaag gaaaagcgca aggcggcaaa atccaagcgg aagacgccgc gcctggcgga
      661 cggccagcag cctggcggca tgaaaccggt cggcggccag cgccaggaaa gcccctatcg
      721 ccagcagcag gccctgatgg ccgagccgct gtcgctggcg gatttgctgc gcgacgacct
      781 tgacgaaggc aacctggcgg cagaagaggt tgccgataca ggaggcattc ccgcttctgc
      841 cgccgagcag gaatcgtacg aggacgcgga gctgcggttt tcgaaggaat cgcagcgcgg
      901 caaaccgggc ggcgtgattg cctacgaggg gggcgaagct tccggcgcgg agccccgtgg
      961 ccgtcgcggc ggcaaaaggc gtcgccgcga tgctcacggc cggatagccg ccgaacagca
     1021 gcccgaaaag ctcccccgcg agagtttcgg caagaagcgg agcggcaagc ggattttcgg
     1081 tcggccccac caggggcgcg gaccggcggg acagacggcg ggcaacttcg attatcgccc
     1141 ggcaggtcaa cccaagaaaa agtcgatcca gaaagacgag cgcccaagcc ggcccgcgaa
     1201 gcggggcaaa cagcgcttcg ccaaagcagc cggcggaaaa ttcggcaagc ccggcggcgg
     1261 aaagaagttc gtcgggcgca gcaagcggaa gggacgccga tgaacttgca ggccagcccg
     1321 cttcatgccc gattctgccc ggatcaagcg gtgcagctgc cggctgtgat cgtcgagaac
     1381 gtgcgcctgg ccagggacac gttccgcgtc cgcttacatg ccccggaaat cgcccgccgg
     1441 attgtccccg gacagttcgt aatggcgcgg attgccggcc tcaacgatcc gctcatcggc
     1501 cggccgctgg cgctgtacga cacggtgatc gggaccgatg gacagccggc agccatcgat
     1561 cttgtgtacc tcgtgaaggg caaactgacg acgcggctct gcggcatgca gcccggacag
     1621 ctgctcgatg tatgggggcc gctaggaaac ggatttggcg ccgaaggttc aggggccggg
     1681 gatcagggcg ccgagggcgg ggttcagggt tcagggatca gggttcaggg gccggagatc
     1741 ggggctcgga cctcggatgc cggtgaaggc gtgccgcgct caattcacca ccggctggct
     1801 gccgtgggtc tccgggggaa gcgattgcac gcagccaggc agcacctcat tctcgtcgcc
     1861 ggcggtattg ggcagacgcc gtttctggca ttggcccagg aagcgttgca cttgaagcgc
     1921 tatggcgaac cgccgcgcga agtgccgcgc gccgaacgca tcaccttttg ctacggcgct
     1981 cgcaccgccg actacctggc cggggtcgag gacttcgagc gactgggcat cgacgtgcgg
     2041 attgccaccg acgacggcag ccgcgggcat ccgggccttg tgacggagtt gctcgagcaa
     2101 gtc
//
LOCUS       DASUGB010000594         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1872488, whole genome shotgun sequence.
ACCESSION   DASUGB010000594 DASUGB010000000
VERSION     DASUGB010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872488"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFV87_11355"
     CDS             <1..248
                     /locus_tag="VFV87_11355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5104402.1"
                     /translation="RLYLHEPLAEPVEETWCGWRPMTPDSLPIIGRGPALANVWIAAG
                     HNMLGLSLAPGTGRLLAELITGEDPHVLASPFLPGRF"
     gene            complement(270..1337)
                     /locus_tag="VFV87_11360"
     CDS             complement(270..1337)
                     /locus_tag="VFV87_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104403.1"
                     /translation="MNIDTTLLLKKYEPAKEAQVITAEPQVRAARFSPCGKVLLAGGF
                     DGRVRRWNFAADGDPPEMPALEGHHAWIDGLAFRQDGELLFTADSWGQLCCRSGYPTD
                     QPQEKWRVEAAHDGWIRDLAVSHDGQRLASCGSDRCVRLWSVDEGTKLGEIAAGRDVL
                     RLVWMPDGSLLSGDDRGIVKLWKDDGTLVRQFDASPLYALSRLQDVGGVHALAIDREG
                     KLIAAGGTIPKNGATVVGPPTILIFDATTGEQKHKLTLGADNDCYVADMHFHEEGFLS
                     AVTYGTPGQGQLLYVLPQEGKPFFTRKLSNLHSLSWHPDGQRLAVVATNPGSNGNGRP
                     LDKDGNYRGNKSPIHIFRTGG"
     gene            complement(1334..1879)
                     /locus_tag="VFV87_11365"
     CDS             complement(1334..1879)
                     /locus_tag="VFV87_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104404.1"
                     /translation="MSIRRIAHLVCLLGLVALVQAPPLEAAEPQQVLLPAPLDAVALD
                     PGPFGEKFDVVKIEHFGPGEFTVPPNRVVAEDSLVFTLRAKVAVDKAAMNSLLANPVP
                     RVRFVKQQDGKSLDADARPQGYRLHHDGRWLSARLGPDLKAGESFQVWTHLGKEGAVA
                     LKEKGASQAVWEKNHEPAKKP"
     gene            complement(1923..>2576)
                     /locus_tag="VFV87_11370"
     CDS             complement(1923..>2576)
                     /locus_tag="VFV87_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104405.1"
                     /translation="AAANARYAKNRRRQMGEATSYTFDVARTLMRRQDLFDTSKFDPR
                     DVERYGTHEFGRHMLQARKMLEAGVTFVKVNSYGWDTHGDNFNGHLSLMPKFDQAFAA
                     MVQDLADRSMLGNVLVIAMSEFGRTPRINGHIGRDHWPEAWSVAMAGSGLVRGAVVGQ
                     TNEQGTFVKGDEHDIGALFHTWFHCLGIDSKATQYDNGGQPLPLAHEGMSAVKELLT"
BASE COUNT          422 a          851 c          797 g          506 t
ORIGIN      
        1 cacggcttta cctgcacgag cctttggccg agcccgtcga ggagacgtgg tgcggctggc
       61 gcccgatgac gcccgacagc ctgccgatta tcggccgcgg cccggcgctt gccaacgtct
      121 ggatcgccgc gggccacaac atgctcggcc tgtcgctcgc ccccggcacg ggccgccttc
      181 tcgccgagct aatcacaggc gaagatccgc atgttctggc gagtccgttt ttgccggggc
      241 gtttttgagc gctacttacg gggaaccgat cacccacctg tcctaaaaat gtgaatcggc
      301 gacttgttcc cgcggtaatt tccgtcctta tccagcgggc ggccatttcc gttgctgccg
      361 gggttcgttg ccacgaccgc cagccgctgg ccgtctggat gccacgagag gctatgcagg
      421 ttcgatagct tgcgggtaaa gaagggcttg ccctcctgcg gcagcacata gagtagctgc
      481 ccttggcctg gggtgccgta ggtcacggcc gacaggaagc cttcttcgtg aaaatgcatg
      541 tcggcgacat agcagtcgtt gtcggccccg agcgtgagct tgtgcttctg ctcgcccgtg
      601 gttgcgtcga agatcaggat tgtcgggggg cccacgacgg tggccccgtt tttgggaatg
      661 gtgccgcctg cggcgatcag ctttccttcg cgatcgatcg ccagcgcatg cacgccgccc
      721 acgtcctgca agcggctaag cgcgtacagc ggcgaggcgt cgaactgccg gactagcgtg
      781 ccgtcgtctt tccagagctt gacgattccg cggtcgtcgc cgctgagaag cgacccgtcg
      841 ggcatccaca ccaggcgtag gacgtcgcgg cctgcggcga tttcaccgag tttcgtcccc
      901 tcgtccaccg accagagccg cacgcaccga tcgctgccgc aacttgccag ccgctggccg
      961 tcgtgactga cggccagatc gcgaatccag ccgtcgtggg cggcttccac gcgccatttc
     1021 tcttgcggct ggtcggtcgg gtacccgctc ctgcagcaga gttgccccca ggaatcggca
     1081 gtaaagagca actcgccgtc ctggcggaag gccaggccgt cgatccacgc atgatgccct
     1141 tccagcgccg gcatttccgg cgggtcgcca tcggcggcaa agttccaccg ccgcacccgg
     1201 ccatcgaagc ctcccgccag gagcaccttg ccgcaggggc tgaaccgggc agcgcgcacc
     1261 tgcggctcgg cagtgattac ctgcgcctcc ttcgccggct cgtatttctt cagaagcaac
     1321 gtcgtgtcga tgttcatggc ttcttcgcag gctcgtggtt cttttcccac actgcctgcg
     1381 acgcaccttt ttctttcaag gcaacggctc cttcctttcc caaatgcgtc caaacttgga
     1441 atgactcgcc ggcctttaag tcggggccca gtcgggcgct cagccagcgg ccatcgtggt
     1501 gcaatcggta gccttgcgga cgggcgtccg cgtcgagcga cttgccgtcc tgttgtttca
     1561 caaaacgaac ccgcggcacg ggattggcca agagcgagtt catcgcggcc ttgtccacag
     1621 ccaccttggc acgcagggta aaaaccagcg agtcctcggc gacgactcga ttgggcggca
     1681 cggtaaactc gcctggcccg aagtgctcga tcttgacgac gtcaaacttc tcgccaaacg
     1741 gccctggatc gagagcaacg gcatccagag gcgccggcag cagcacctgc tgcggctcgg
     1801 cagcttccaa tggcggcgct tgtacgagcg ctaccagccc gagcagacaa accaaatgtg
     1861 caatgcgccg aatggacatt ggatagttcc cgatcgtgaa atggtcagtc gagacgtgat
     1921 cgctaggtca acaattcctt caccgccgac attccttcgt gcgccagcgg cagcggctga
     1981 ccgccgttgt cgtactgtgt cgctttgctg tcgattccca ggcagtggaa ccaggtatgg
     2041 aagagcgcgc cgatgtcgtg ctcgtcccct ttgacgaacg ttccctgctc gtttgtctgg
     2101 ccgacgaccg ccccgcgcac caggccgctg ccggccatgg ccaccgacca ggcctcgggc
     2161 caatgatcgc ggccgatatg cccgttgatc cgcggcgtgc ggccgaattc gctcatggcg
     2221 atgaccagca cgttgcccag catggagcgg tcggcgaggt cctgcaccat cgccgcgaag
     2281 gcctggtcga acttgggcat caggctgaga tggccgttga agttgtcccc gtgcgtgtcc
     2341 cagccgtagc tgttgacctt gacgaaagtg acgccggctt cgagcatctt acgggcctgc
     2401 agcatgtggc ggccgaactc gtgggtgccg tagcgctcga catcgcgcgg gtcgaacttg
     2461 ctcgtgtcga acaggtcctg ccgccgcatg agcgtgcggg ccacgtcgaa cgtgtagctg
     2521 gtcgcctctc ccatttgccg gcgacgattc ttggcatagc gggcattggc cgcggc
//
LOCUS       DASUGB010000595         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1596196, whole genome shotgun sequence.
ACCESSION   DASUGB010000595 DASUGB010000000
VERSION     DASUGB010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596196"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..218
                     /locus_tag="VFV87_11375"
     CDS             33..218
                     /locus_tag="VFV87_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5104406.1"
                     /translation="MNRNFLKHVRHFLASEDGPTAVEYAVMLALIVIVCLVSSQAIGT
                     RANATFNSIAQALPAGS"
     gene            554..1123
                     /locus_tag="VFV87_11380"
     CDS             554..1123
                     /locus_tag="VFV87_11380"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5104407.1"
                     /translation="MWESCSALAATIAENWPIWLVTIVLIVSAVIDGFELRVPNLITY
                     PFILSGWAYSFFAFGWEGLGWSLAGTLLGMSVLMLFYAIGGMGAGDVKLMGGVGAWIY
                     WTNTYYCIIGFAVIGAVLAVAMVAVRGNWKHHYHQFWVIFHEITVIRDPEKLSAIAAE
                     RKPTMMLLPYGIPIAIATIGYFLWMGMLV"
     gene            1120..1647
                     /locus_tag="VFV87_11385"
     CDS             1120..1647
                     /locus_tag="VFV87_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX5104408.1"
                     /translation="MNLKRCLSLANESSLPGWQTGQAVPSLRPTSEKLEKPCRSYRRN
                     RRAAAAVEFALVAPVFFLLILGMVEYGRMVMVQQVITNASREGARRAVLDGATTSEVQ
                     TLAKDYMTSAGVSPTGATVTVAPNPPNNAAFGEAVTVTVAIPFSQVSWLPSPMYLGGR
                     TLTASTVMRREDTVP"
     gene            1766..>2627
                     /gene="cpaB"
                     /locus_tag="VFV87_11390"
     CDS             1766..>2627
                     /gene="cpaB"
                     /locus_tag="VFV87_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX5104409.1"
                     /translation="MKSLILIFIALGCGLVASIGISQVMDRGGGGGNVEMEQILVALA
                     DIDIGSKLDAQNVKLEDWPKAKVPEGAVRRLEDVKEKFANSRFFKGEPIHTSRITDNL
                     KSVANSVPEGFRAMPVKVDEDTVMKAISPGDRVDVMVFLRKNEEIPETGVFTILKNVR
                     VFAVNTNTERNNEASGKNETANFRTVSLLVTPDQSRDLAVAAQIGKILLTLRRADEQD
                     DKSGEEATNINLLLGGHPKIASDPLKQGSTIPQFSTPKHGSSSAPANPPEFFMELWTP
                     TSMEQFNWYDR"
BASE COUNT          520 a          825 c          791 g          491 t
ORIGIN      
        1 ttcgatttcc tgcacgcatc aagggttcgt ttatgaatcg caatttcctg aaacatgtcc
       61 gccacttcct ggcgtctgaa gacggcccga ccgccgttga atacgccgtg atgttggccc
      121 tgatcgtcat cgtttgcctc gtctcgagcc aggcgatcgg cacgcgggcc aacgccacct
      181 tcaacagcat cgcgcaggcc ctgccggccg gctcgtaagc tgaagcgttc tgccaacttg
      241 ggcggcttgt ccctgcgctg ggacagccgc ccttgtcggc attcttgacc cgacccaccg
      301 agcacgaaag tccggagtta tccatgttgc cgattttgcc gggcgagctt ttcaccggcg
      361 gcctggaact cgtgtgctac ggtttcacca tcctggccgc gctggcgagc tacctgctcc
      421 tgctgcgctg accggcgggc gcatggcctg cggcgggccg ccacaggggc cgtctgccgc
      481 tgggaaatca ccgctttgag cgacgccagc ccaccagcca caacgattca agtatctcgg
      541 gagaaagcaa gtcatgtggg agtcgtgctc ggccctggcc gccacaatcg ccgaaaactg
      601 gccgatttgg ctggtgacca tcgtcctcat cgtgtcggcc gtcatcgacg gcttcgagct
      661 gcgggtcccc aacctgatca cctacccgtt catcctcagc ggctgggcct acagcttttt
      721 cgccttcggc tgggaagggc tcggctggag cctggccggc acgctcttgg gcatgagcgt
      781 gctgatgctg ttttacgcga tcggcggcat gggcgccggc gacgtgaagc tcatgggggg
      841 cgtgggcgcc tggatttatt ggaccaatac gtactactgc atcatcggct tcgccgtgat
      901 cggcgcggtc ctggcggtcg ccatggtggc cgtccggggc aactggaagc accactacca
      961 ccaattctgg gtgatcttcc acgagatcac cgtcatccgc gatccggaaa agctctcggc
     1021 catcgccgcc gagcgcaagc cgaccatgat gctcctgccc tatggcattc cgatcgccat
     1081 cgcgacgatc ggctacttcc tgtggatggg aatgctggta tgaacctgaa acgctgcttg
     1141 agcttagcga acgaatcaag tctgccgggc tggcaaactg ggcaagctgt gccgagtttg
     1201 cgccccacca gcgagaaact ggaaaaacct tgccggtcgt accgaagaaa ccgacgggca
     1261 gcggccgcgg tggaattcgc gctcgttgcc ccggtgtttt tcctgctgat cttgggcatg
     1321 gtcgagtacg gacgcatggt catggttcag caggtgatca ccaacgcctc gcgcgaggga
     1381 gctcgccgag cggtgctcga tggtgcgacg acctcggagg tccagacgct ggccaaggac
     1441 tatatgacca gcgcgggggt ttctccgacg ggcgcaacgg taacggttgc tccgaatccg
     1501 cccaacaatg cggcttttgg cgaagctgtt accgttacgg ttgccattcc tttcagtcaa
     1561 gtaagttggc tcccttcgcc aatgtacttg gggggaagaa ccctgacagc aagcactgtg
     1621 atgcgccgcg aagatacggt gccgtaggaa cctgcggccc cgggagcgac acaaacccag
     1681 cagcaggcga gaggcccagc cgctcgccgg caactgctga tcctcagtag cgagactcga
     1741 ttggcaaagg gaacgtacga tgcgcatgaa atcgctgatc ctgattttca tcgccctggg
     1801 ctgcggcctg gtggcgtcga tcggcatcag ccaggtcatg gatcgcggcg gaggcggcgg
     1861 caacgtcgag atggagcaaa tcctcgtcgc cctggccgac attgacatcg ggtcgaagct
     1921 cgacgcgcaa aacgtcaagc tcgaggactg gccgaaagcg aaggtgcccg aaggggccgt
     1981 ccgccgcctg gaagacgtga aggagaagtt cgccaactcc cgcttcttca agggagagcc
     2041 gattcacacc agccgcatca ccgacaacct caagagcgtg gccaactccg ttcccgaggg
     2101 cttccgggcg atgcccgtga aggtggacga agacacggtg atgaaagcca tctcccccgg
     2161 cgaccgggtg gacgtgatgg tcttcctccg caagaacgaa gagattcccg aaaccggcgt
     2221 atttacgatc ctgaagaacg tccgcgtttt cgcggtcaat accaacaccg aacgcaacaa
     2281 cgaagccagc ggcaagaatg aaaccgcgaa cttccgcacg gtttcgctct tggtgacgcc
     2341 cgatcaatcg cgcgacctcg cggtcgccgc gcagatcggc aagatcctcc tgacgctccg
     2401 ccgtgcggat gaacaggacg acaaatcggg cgaggaagcc acgaacatca acttgttgct
     2461 gggcggacat cccaagatcg ccagcgatcc cctcaagcag ggctcgacga tcccgcagtt
     2521 cagcaccccc aaacacggat cgtcgtccgc tccggccaat cccccagagt tcttcatgga
     2581 gctctggact ccaaccagca tggagcaatt caactggtat gaccgga
//
LOCUS       DASUGB010000596         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_484436, whole genome shotgun sequence.
ACCESSION   DASUGB010000596 DASUGB010000000
VERSION     DASUGB010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484436"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFV87_11395"
     CDS             <1..592
                     /locus_tag="VFV87_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5104410.1"
                     /translation="CAIAGGDVNSWEGPLVISVTALGEVQPGREWRRSGARPGDQILV
                     TGSFGGSILGKHFEFVPRVAEALWLAENAEIHAAIDVSDGLSLDLARLCEASGCGAEI
                     DPQKIPIAPAAAKRTANGPGGRSPLEHALSDGEDFELILAVPPECAAESLRRQPLDLP
                     LTAMGRFVERPGLYALGTSGEYEPLVPRGYEHRLDQ"
     gene            589..1053
                     /gene="tsaE"
                     /locus_tag="VFV87_11400"
     CDS             589..1053
                     /gene="tsaE"
                     /locus_tag="VFV87_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5104411.1"
                     /translation="MNELTFDAQSESDTDRLGAALAAVLPPGTTVALVGTLGAGKTRL
                     VQAIAAGCGVPREEVVSPTFVLCQEYEGARTIHHLDAYRLRDEDEFRELGVEELFHSG
                     GLTIIEWADRVAGCLPDERVEIAIEVTGETQRRFRIRTIGDRLDSAISKLAR"
     gene            complement(1389..2072)
                     /locus_tag="VFV87_11405"
     CDS             complement(1389..2072)
                     /locus_tag="VFV87_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104412.1"
                     /translation="MRFGLTLSAVVCFAPAGAALADDAPKLEIAAGAAVRFASVEEGR
                     TVLQSDDAFTRSLSPFDLQSRMKSAEKVQLEAWKDFVAQHVRPWEEEDVKAVTESVTR
                     LSKRLAKYRLPLPKEIVLVRTSGEEEGNAAYTRGTAIVLPDKVLKYSPQQLDRLLTHE
                     LLHILSRHDGALRQRLYRIVGFEVCDPIAMPESLAPRKITNPDAPLVDCVIELTADDG
                     QTYTAAPVL"
     gene            complement(2407..2658)
                     /locus_tag="VFV87_11410"
     CDS             complement(2407..2658)
                     /locus_tag="VFV87_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104413.1"
                     /translation="MFRFMIRDVLWLTVVVALAVGWWLDNRSTAAALAKTERRAEELS
                     VQNGELLQERTKFHNMARAATRELEKATGQQLIWEYRED"
     gene            complement(2668..3198)
                     /locus_tag="VFV87_11415"
     CDS             complement(2668..3198)
                     /locus_tag="VFV87_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104414.1"
                     /translation="MADHPRTRWRFSLAKLILVLTVYASVTAVVAALPVTQIAAALAV
                     VTVFVAIDLGLGTQCLGGSTRVRLIVMTIAHYSTAFAYAGAACGFVYFWTLESQQIGN
                     LPDPVLAVQGAFSELATLVVAFAFYWAVSTFFAAIGGIAALSSIRHSRRSVLLLMANV
                     PWLVFFIYALASQSTE"
BASE COUNT          637 a         1050 c         1049 g          601 t
ORIGIN      
        1 ttgcgcgatc gccggcggcg atgtgaatag ctgggaggga ccgctggtca tcagcgtcac
       61 agctttgggt gaagtccagc ctggccgcga atggcgacga agtggcgctc ggccaggaga
      121 ccagattctc gtcacgggca gctttggcgg cagcatcctg ggaaagcact tcgagttcgt
      181 tccgcgcgtc gcggaagcgc tgtggctggc cgagaacgcg gagattcacg ccgcgatcga
      241 tgtcagcgac ggactctcgc tcgatctggc ccggctgtgc gaagccagcg gctgcggggc
      301 ggaaattgac ccacagaaaa taccgatcgc ccccgcggca gcgaaacgga ccgcgaatgg
      361 accaggcgga cgatcgccct tggagcatgc cctctccgac ggcgaagatt tcgagctgat
      421 cctcgcggtg ccgcccgaat gcgcagccga atcgctccgc cgccagccgc tggacttgcc
      481 gctgacggca atgggccggt ttgtggaacg acccggacta tacgcgctgg gcacaagcgg
      541 agaatacgag ccgctggttc cccgcggcta cgagcatcgc ctggaccaat gaacgagctt
      601 accttcgatg cccaaagcga aagcgacacc gaccggctcg gggcagcctt ggccgccgtg
      661 ctgccgccgg gaacgaccgt cgcgcttgtg ggcacgctcg gggccggcaa gacgcggctg
      721 gtgcaggcga tcgcagccgg ctgcggcgtg ccgcgcgagg aggtcgtcag ccccacgttc
      781 gtcctctgcc aagagtatga aggggcacgg acgatccatc acctcgacgc ctatcgcctg
      841 cgagatgaag acgaattccg cgaactgggc gtggaggaat tgttccattc cggcggcctc
      901 acgatcatcg aatgggccga ccgcgttgcc ggctgtctgc cggacgagcg ggtcgagatt
      961 gcgatcgagg tcacgggcga gacccagcga cggttcagga ttcgaacgat cggtgaccgc
     1021 ctggactccg cgatttcgaa gctagccagg taattctggt gggcctgcgg ccgcggcggc
     1081 cttgtcccac cctacggcgc gagcgcccgc ttcatctgct cgatgatccg gggggtcatc
     1141 aggtcggcgt cgtccatcac gaggtggacg aagttgtcgg ccaggatttc gtcggggtgg
     1201 atgatgtagt tcgtgttctg gccgatcttt tccaggtaag cgggctcctt tttcgcgtcg
     1261 atgacgatcg gctcgccctt caggagccgc ggctgccagc ggttcccctt cttctcaacg
     1321 accagcaggc ggaacgtgag atactggaac agcgttccgc cgcgggcggc gtcgtagtcc
     1381 tgcgtcgcct acagcacggg ggcggccgtg tacgtctgcc cgtcgtcggc tgtcagctcg
     1441 atcacgcaat cgaccagcgg ggcgtcggga ttggtgatct tccgcggcgc gagcgattcg
     1501 ggcatcgcaa tgggatcgca cacctcaaag ccgacgatcc ggtacagccg ctgccgcagg
     1561 gcgccgtcat ggcggctgag gatgtggagc agctcgtggg tcaggaggcg gtcgagctgc
     1621 tgcggcgaat atttcagcac cttgtcgggc aggacgatcg ccgtgccgcg ggtgtaagcc
     1681 gcgttccctt cctcctcgcc gctggtgcgg accagcacga tctccttcgg cagcggcagg
     1741 cgatacttcg ccagccgctt gctcagccgc gtgaccgact ccgtcaccgc cttgacgtct
     1801 tcctcttccc agggccgcac gtgctgggca acgaagtcct tccacgcctc aagttgcacc
     1861 ttctccgccg acttcatccg gctctgcaga tcaaacgggc tcagactccg cgtaaaggca
     1921 tcgtcgctct gcaaaaccgt ccggccctcc tcgactgaag cgaaccgcac ggcagcccct
     1981 gcggcgattt ccagcttagg cgcgtcgtca gccagtgccg cgccggctgg cgcaaagcaa
     2041 acgacggcgg aaagtgttaa accgaatcgc atcaagagct attgtagccg tcgggtggtc
     2101 cgggtggctg ggttttgcgc agcataaccc agcgaatcgc cactcatgct aacatagctc
     2161 cacacggccg acgcccggcg cgtggaagta gtccggcgcc gcggccttcg aaggcgcctg
     2221 cactatcatt cctcttgatc ccattccttg ggactggctg gccgacgcct gaatcgatcg
     2281 tcgcgccggc tgggtcatgc ttcgcaagac ccggccaccc tgcccgctag cgccaccacc
     2341 accgtcagcc atagcacgtc acggatcgtg aaacggaaca tgagaatact ctttggttgt
     2401 ggacggctaa tcctcgcgat attcccaaat cagttgctgg ccggtggcct tttctagctc
     2461 gcgcgtggct gcgcgggcca tgttgtggaa cttcgtccgc tcctgcaaca actcgccgtt
     2521 ttggacggac aactcctcgg cgcgacgctc ggtcttcgcg agcgccgcag ccgtactccg
     2581 gttatccaac caccacccta ccgccagcgc gaccaccacc gtcaaccaca gcacgtcgcg
     2641 gatcatgaaa cggaacatga ttggtttcta ttcggttgat tgtgaggcaa gcgcgtaaat
     2701 aaagaatacc agccacggca cattcgccat caggagaaga acgctccggc gactatggcg
     2761 gatcgaggac aatgcggcaa ttccaccgat tgccgcaaag aatgtgctaa ccgcccagta
     2821 gaaagcaaag gctacgacga gcgtggcgag ttcactaaat gcgccttgaa cggccaaaac
     2881 cggatcgggc agatttccga tctgttgact ctctagcgtc caaaagtaaa cgaaaccgca
     2941 tgccgcgccc gcatatgcaa aagcggtgga atagtgagca atcgtcatca cgataagtcg
     3001 cacccgcgtg ctgccgccga ggcattgtgt cccaaggccc aggtcaatcg cgacgaagac
     3061 cgtaacgacc gccagcgccg ccgcgatttg cgttacggga agcgcggcga ctaccgctgt
     3121 taccgaagcg tacaccgtga gcacgaggat tagcttcgcc agtgagaatc gccaacgagt
     3181 gcgggggtga tcagccatat cgttcacgat aacagggtgc ggatgtggca ggccagaaat
     3241 gcgcgacgct gatggcgtcc taatctgaag ggtggtcgac gaattggacc cgcgttacgg
     3301 gcggtcatcg ccgatcagag tagtcgacca gttcgtg
//
LOCUS       DASUGB010000597         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_206595, whole genome shotgun sequence.
ACCESSION   DASUGB010000597 DASUGB010000000
VERSION     DASUGB010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206595"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VFV87_11420"
     CDS             <1..1332
                     /locus_tag="VFV87_11420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5104415.1"
                     /translation="FDVRQATDDRQALAAAQSARPDFLVLDTSHGDPVDLQLCRQVRR
                     IWPQGYTFALLLAERPETADITTALEAGFDDFLTAPVVFGELLARLRAGARVIEFEQR
                     LAEQSGLEPTTGLPDKGSLSTEFATRLQQATGAIGWLALFDIDYFFRFADQCGRPAAQ
                     AVLREAGQLIQQQLSPGGFVASLASDQIVILLPPGASEAIVVWADGALAKVAEHEYTL
                     GGEKVALSASCGATEISPGESLDAVLVRAQKSLQFAKSSGRNCTATSQEVDREAEAWA
                     AFAADGKLFQTTVARDVMQPCPLLLHVDETVDQASAFIQLTGLTVAPVVDGDGKLAGT
                     VTLDQLAAARLRSDIKTRESGSVRLVRHVMSTDIVKFDETTSLGRLFEFFTGENAPLA
                     IVVRDRCPRGIVHCQGLAALNEKLAAGYFAATKPRTNTSEDLLVPELAMAE"
     gene            1468..>2205
                     /locus_tag="VFV87_11425"
     CDS             1468..>2205
                     /locus_tag="VFV87_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEX5104416.1"
                     /translation="MQIVVFEDEHVSRLYPITVGKPACAISCATYRLIDWLGALSRET
                     GIVVRGVVRPHLSDVQRLDFPQIGPTVPTAGTPLLLVNARLVPSVGAYRALRQLLHGL
                     ETRAVYAGQALAAAMIGPRGPAPPPDDQVDHWHKYLRNSVTGQLPASDVLLPLIDYPH
                     DLIRHNLEQFGESLNHRLSTGSYQEIAPGVFAAEGATLGQYCVTDTSKGPVLLDTGAS
                     VGPYCLLRGPAYVGAKSRINEHSAIKDA"
BASE COUNT          381 a          719 c          728 g          377 t
ORIGIN      
        1 ttcgacgtgc gacaggcgac cgacgaccgg caggcgctgg ccgcagccca gtcggcgcgg
       61 cctgactttc tggtcctgga taccagccac ggcgatccgg tcgatctgca gttgtgccgg
      121 caggtgcggc ggatctggcc gcagggatac accttcgcgc tgctcctggc cgagcggccg
      181 gaaacggcgg acatcacgac cgcgctggaa gcgggctttg acgatttcct caccgctccg
      241 gttgtcttcg gcgaactgct tgcacgactt cgcgccggtg cacgcgtgat cgaattcgaa
      301 cagcgcctgg ccgagcagtc ggggctcgag ccaaccaccg ggctgccaga caagggctcg
      361 ctctccacgg aattcgccac ccgcctgcag caggccaccg gcgccatcgg ctggctggcg
      421 ttattcgata tcgactattt cttccgcttt gccgaccagt gcgggcggcc agccgcccag
      481 gcagtgctgc gcgaggcggg ccaactgatt caacaacagc tttcgcccgg aggattcgtg
      541 gcctcgctgg cgagcgacca gatcgtcatc ctcctgccgc cgggcgcgag cgaagcgatc
      601 gtcgtctggg ccgatggggc gctggccaaa gtagcggagc acgaatacac gctcggcggc
      661 gagaaagtcg cgctctcggc gagctgcggc gcgacggaga tttcgcctgg cgaatcgctc
      721 gacgcggtgc tcgtacgagc gcagaagagc ttgcagttcg ccaagtcctc gggacgcaat
      781 tgcacggcca ccagtcagga ggtcgaccgg gaggcggaag cctgggcggc gtttgccgcc
      841 gacggaaagc tgttccagac gaccgtggcc cgcgacgtga tgcagccttg cccgcttctg
      901 ttgcacgtgg acgagacggt cgatcaggcc agcgccttca tccagctcac gggattgacg
      961 gtcgcgccgg tcgtcgatgg cgatgggaag ctcgcgggaa ccgtcacgct cgatcaatta
     1021 gcagccgcgc ggcttcgcag cgacatcaag acccgcgaaa gcggctccgt gcggctggtg
     1081 cggcacgtga tgagtacgga catcgtgaag ttcgacgaaa cgacgtcgct cggccgcctg
     1141 ttcgaattct tcaccggcga aaacgccccg ctggcgatcg tcgtccgcga ccgctgtccg
     1201 cggggaatcg tgcattgcca gggactggcc gcgctcaacg agaagctggc cgcgggctac
     1261 ttcgccgcca ccaagccgcg gacaaatacg agcgaagatc tgctcgtgcc ggaattggcg
     1321 atggcggagt gagcggcaag tgcggaatgc ggaattcgga gtgcggaacg aagaatttcg
     1381 cgcgacgatg ctcttggtga gcccgctgat cgcgcgctag acttgctgta attccgcact
     1441 ccgaattccg cgttccgcac tcccagcatg cagatcgtcg tcttcgagga cgagcacgtt
     1501 tcacggctgt atccgattac ggtcggcaag ccggcctgcg cgatctcctg tgccacgtac
     1561 cggttgattg attggctggg cgccctcagc cgcgagacgg gcatcgttgt gcggggcgtg
     1621 gttcgcccgc acctgagcga cgtgcagcgg ctcgacttcc cgcagatcgg cccgaccgtg
     1681 ccaacggcgg gcacacccct cttgctggtc aatgcccggc tggtcccttc ggtcggcgcc
     1741 taccgagcgc tgaggcagct tttgcatggc ctcgagacac gcgcagttta tgccggccag
     1801 gcgctggctg cggcgatgat cggcccgcgc ggtccggcgc cgccgccaga cgaccaggtc
     1861 gaccactggc acaaatacct gcgaaactcc gtcaccggcc aactgcccgc cagcgatgtg
     1921 cttctgccgc tcatcgacta cccccacgac ctgattcgcc acaacctgga gcagttcggc
     1981 gagagcttga accatcgatt gagcaccggc tcgtaccagg aaattgcccc aggtgtgttc
     2041 gccgcggaag gggcgacgct tgggcagtat tgcgtgaccg acacgagcaa aggcccggtc
     2101 ttgctggata ccggcgcgag cgtcggcccg tattgcctct tgcgcgggcc ggcttatgtg
     2161 ggggcgaaat cgcgaatcaa cgagcactcg gccatcaagg acgca
//
LOCUS       DASUGB010000598         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2012562, whole genome shotgun sequence.
ACCESSION   DASUGB010000598 DASUGB010000000
VERSION     DASUGB010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012562"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1138
                     /locus_tag="VFV87_11430"
     CDS             173..1138
                     /locus_tag="VFV87_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104417.1"
                     /translation="MSATLPAATADKVQPVRMGCGLMTFDTVPGWGLRPDGTSALGPT
                     HGGVVVDKEGNVYVSAGKGVVVFTSDGQVIKEYLGDAYAQIHDLEIRDEGDAEYIYGA
                     RNNNAEGIKFNAKTGEIVLKLPFPKESGLNLMKFNPTAITVAPNGDIFLADGYASNHI
                     FKFDKTGKYLMHFGVKGNELQQFNTCHGMTLDTRYEPPRLLICDRNHTPKGRLLHYDL
                     EGNFIEEVITGLGMPTSAAVQGDYVSVPDLHGRVVILDKSNTIMAVLGHNPDPAKGRN
                     YNIPQDQWQEGIFSGTHGSYWDQQGNLYVQDWNVAGRIIKLARVK"
     gene            complement(1264..1629)
                     /locus_tag="VFV87_11435"
     CDS             complement(1264..1629)
                     /locus_tag="VFV87_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5104418.1"
                     /translation="MIRGVHTMFYSSQAQALRAFIRDVLGWTYTDVGEGWLIFDAPEA
                     DLGVHPADETQPHARAGTHAISFYCDEIEKTVADLQAKGVEFTMPIEDHGYGLVTYFK
                     MPGEVLVQLYQPHYTKRRK"
     gene            1743..>2600
                     /locus_tag="VFV87_11440"
     CDS             1743..>2600
                     /locus_tag="VFV87_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104419.1"
                     /translation="MSRINIWTAAVAVLIVVPFVAGPALGQETLEQYLERQSRERQAA
                     ALRQERWAEARGREFAAWRAAKAVAEDPLLELPGADLPPGKYSIRLPFGVTRRVVVDE
                     PATVPAEVTLSEQDYANIYSYAHRLNRQLADIQLELSRLQEDDLTGEATRVHDESLNF
                     TAAVTTRQPQTDLQRHFADFDRMWQPLAYHLATRENLGVFVRRAAGDVEALATALNQL
                     LTATAVMATYDRPVVASLTDELVRATANLVEDLQITRLQSVHRDRLEALALRAQRQAA
                     DLDATVKTNA"
BASE COUNT          504 a          759 c          884 g          453 t
ORIGIN      
        1 gatccccgcg tcagggcgag cgaggcgact agttgatttc cgccagccgc ggttggatac
       61 tgagcgcctc acacgggcga tcgattcgat ttgggagcca acatccatga agcggggtta
      121 ttcgtatccg gccagcatcg cgattctttg cacactgtcg gccgcagcca ctgtgtcagc
      181 cactttgccg gctgccaccg ccgacaaggt gcagccggtg cggatgggct gcgggctgat
      241 gacgttcgac acggtgccgg gctgggggct gcggccggac ggcacttcgg ccttgggacc
      301 gacgcacggc ggggtggtgg tcgataagga aggcaacgtg tatgtcagtg cgggcaaggg
      361 ggtcgtcgta ttcacctccg acggccaggt gatcaaggaa tatctgggcg atgcgtacgc
      421 gcagattcac gatttggaga ttcgcgacga aggggacgcg gaatacatct acggcgctcg
      481 caacaacaac gccgagggga tcaagttcaa tgcgaagaca ggcgaaatcg tcctcaagct
      541 gccgtttccg aaggaatcgg ggctgaacct gatgaagttc aatccgacgg cgatcaccgt
      601 ggcgcccaat ggcgatattt tcctggccga cggctatgcg agcaatcaca tcttcaagtt
      661 cgacaagacc ggcaagtacc tgatgcactt cggcgtcaaa ggcaacgagc ttcagcagtt
      721 caatacctgc cacggcatga cgctcgacac gcgctacgag ccgccgcggc tcttgatctg
      781 cgaccggaac cacacgccca aggggcggct cttgcactac gacctggagg ggaatttcat
      841 cgaggaggtg attacgggcc tgggaatgcc gacctcggcc gcggtacagg gggattacgt
      901 ctcggtgccc gatctgcacg ggcgggtggt gattctcgac aagagcaaca cgatcatggc
      961 ggtgctgggg cacaaccccg acccggcgaa aggccgcaac tacaacatcc cgcaagacca
     1021 gtggcaggaa ggcatcttca gcggcacgca cggctcgtat tgggatcagc agggcaatct
     1081 gtacgtgcag gattggaacg tcgcggggag gatcatcaag ctggcgcggg tgaaatgacg
     1141 tagcttgctt gcgttggtgg tagccgcact cgccggagtg cggggggcac agcggagaag
     1201 cccgcgttct ggcgaacgcg gctacgcaag ttgctaggtg gggccgggca cgacgcagcc
     1261 gcgctacttg cggcgcttgg tgtagtgcgg ctgatagagc tgcacgagca cttcgcccgg
     1321 catcttgaaa tacgtgacca ggccatagcc gtggtcttcg atgggcatcg tgaactcgac
     1381 ccccttcgct tggaggtcgg cgaccgtctt ctcgatctcg tcgcaataaa acgagatggc
     1441 gtgcgtgccg gcgcgggcgt gcggctgcgt ttcgtcggcg ggatggacgc ccaggtcggc
     1501 ttcgggggcg tcgaagatca gccagccttc gcccacgtcg gtgtacgtcc agccgaggac
     1561 atcgcggatg aacgcccgca gggcctgggc ctgcgagctg tagaacatgg tgtgaacgcc
     1621 gcggatcatt tgggtagcgc cttagaaaag caagctgtgc ccagcgggga tcgccgggca
     1681 tacggattgc agcattgtag ctctccagcg cttgaaggag acccgaggaa agggagagag
     1741 ccatgtcgcg aatcaatatc tggacggcgg ctgtagcagt cttgatcgtc gtgcccttcg
     1801 tcgccggtcc ggcgctgggg caggagactt tggaacagta cctcgagcgc cagtcgcgcg
     1861 agcggcaagc ggcggctctg cgacaagagc ggtgggccga agcgcggggc cgcgaatttg
     1921 cagcgtggcg cgcggccaaa gcggtcgcgg aggatccgct gctggaattg ccgggcgcgg
     1981 atttgcctcc ggggaaatat tcgattcgct tgccctttgg ggtgacgcgc cgcgtcgtcg
     2041 tcgatgagcc cgcgacggtg cccgcggagg tcacgttgag cgaacaagac tacgcgaaca
     2101 tttattcgta cgcgcaccgc ctgaaccgcc agctcgccga tatccagctc gaattgagcc
     2161 gcctgcagga ggacgacctg acgggcgagg cgacgcgcgt ccacgatgaa agcctgaatt
     2221 tcacggcagc cgtcaccacc cggcagccgc agacggacct gcagcggcat tttgccgatt
     2281 tcgaccggat gtggcagcct ttggcctatc acctggcgac gcgcgagaac ctgggcgtct
     2341 ttgtgcggcg ggccgcgggc gacgtcgaag cgctggcgac ggctctgaat caattgctca
     2401 cggcaacggc cgtgatggcg acctacgatc ggccggtcgt cgcgtcgttg accgacgaac
     2461 tcgtgcgggc gacagccaat ctcgttgagg acctgcagat cacgcgcctg caatcggtcc
     2521 accgcgatcg attggaagcg ctcgccctgc gggcccagcg gcaagcagcc gatttggatg
     2581 ccaccgtcaa gaccaacgca
//
LOCUS       DASUGB010000599         8621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1596486, whole genome shotgun sequence.
ACCESSION   DASUGB010000599 DASUGB010000000
VERSION     DASUGB010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596486"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFV87_11445"
     CDS             complement(<1..357)
                     /locus_tag="VFV87_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5104420.1"
                     /translation="MPTLTQPPITRPAPRGTALLQSLYAPIADELAQVETLLQSELRS
                     EHAYVDELVGYGCLLGGKRLRPALLLLAAKAVGGKVTHEHLTLATVVEMIHTATLVHD
                     DVLDEALMRRHLATVNA"
     gene            complement(439..939)
                     /gene="moaC"
                     /locus_tag="VFV87_11450"
     CDS             complement(439..939)
                     /gene="moaC"
                     /locus_tag="VFV87_11450"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5104421.1"
                     /translation="MPEDLSHFDESGATRMVDVGDKPATERRARASGRVRMQPATLQR
                     IKDRGLAKGDVLEVARLAGIMAAKRTSDLIPLCHPLPLDAVSVSFSFEGEDVLGVEAE
                     VRIVGRTGVEMEALVAVSAATLTIYDMCKAIDRGMVIEQIQLEEKHGGKSGHFRREVP
                     PPPAVT"
     gene            complement(1250..1822)
                     /locus_tag="VFV87_11455"
     CDS             complement(1250..1822)
                     /locus_tag="VFV87_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5104422.1"
                     /translation="MKVFTTGQVAKICKVAPRTVSKWFDSGRLKGYRIPGSQDRRIPR
                     EYLIKFLKEHGMPLGDLEDESMAKVLIVAQDQILIENLRRELPPERQFKVAVAASGFE
                     AGIQAESFHPDCIIADFSIGKVEALQICQNLRKNPDFGETILIALLPDDGSPLSFDRS
                     AINETFKKPFDAKLLAERLRTLIGARKELV"
     gene            2618..3946
                     /locus_tag="VFV87_11460"
     CDS             2618..3946
                     /locus_tag="VFV87_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104423.1"
                     /translation="MTLVRGGILLGLIGLALLGMAGVYLVAADSTPQAAKQKIPLDDW
                     PQWRGVDRSGVSPDSGLNTDWAARPPKLLWTAEGLGGGFASVSLASGVIYTTGNFPDG
                     QAVVAVDAEGGKLLWKQPITDEPPSHSKQGSRSTPSIDGKHVYAIASSGKIVCLNAAD
                     GSEVWSKDFKSEWGGRMMSGWGFSESPLVDGDWVLCTPGGAEAMIVALDKLTGKEIWR
                     CKAPAFGPGKEGAGYSSIVISNACGVKQYVQLVGKGLMGARASDGKFLWGYSDIANRT
                     ANCSTPLISGDYIFCSTGYRTGAALVKLVKSGDSIEAQEQYFKSGEEFQNHHGQMILQ
                     DGYVYAGHGHNNGLPICLELDSGKVVWGGTDRGVGTGSAAVACADGHLVFRYQSGEVA
                     LIEATPSEFRLKGSFKPQFVGRDPCWAQPVIIGGKLYLRDQDKLMCYDAK"
     gene            4050..5105
                     /locus_tag="VFV87_11465"
     CDS             4050..5105
                     /locus_tag="VFV87_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104424.1"
                     /translation="MRRALDEPAFNQQNVGTNSPFAPVNRILRSPAMIFQHIDNLKQQ
                     YTDKYVVVDDSQPELQRFRGLTGTVKTVNYSGKALVEFDGYANIGWYDIDPTFLKVVD
                     APLPKPEEKPAKKEKAAPAAKPAAEKPAAKAAPAKPAPAKPAAGPTAGKSVAEILAAA
                     RKPAGAVAEAPAGEPGASAPGGAAVAPAKVAPGGKGMSMADILAAARSKGGAAPVTPT
                     RSVSESTPAAKAEAPAAKAAASAKPAAAAPAAAAPSKVAPGGKGMSMDDILALARGKK
                     PAAAAPAAPAANSAPASEPLQEPEPASEEPAAEEAAAPAASAAEESAPAGGGGSKKDV
                     ITSVAEQIAYCRKVDAK"
     gene            5221..5769
                     /locus_tag="VFV87_11470"
     CDS             5221..5769
                     /locus_tag="VFV87_11470"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HEX5104425.1"
                     /translation="MSNPSPPPSSGGSSWLIILLVILGLVMLTCAGLCGGCIYLGQRA
                     AVHVGKAAEEFAGYLQLMPAFAATQQAVDNDPQVVQRLGQPIESLAMPKRQNQGDLKP
                     GGETFLYDIRGPQGTAIVSGVATAESPAGPWRVSTINVTFADGSVVTVPTPENQPAGG
                     TFEFQNTEIQLPPELHEPIETK"
     gene            complement(5801..6571)
                     /locus_tag="VFV87_11475"
     CDS             complement(5801..6571)
                     /locus_tag="VFV87_11475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5104426.1"
                     /translation="MDTTKDFAPILSDYAFFEARATEAVNDAAAYARVIAPLVAAGQP
                     LRMLDFGCGTGTFTERLLRLVQIPPPRLQLALVEPVAASPALAAERVARFSASPIEHA
                     NSLAAIQQPDFDLIVANHVLYYVPDLPATLAALIQRLAPGGRLLTAIAGNDNTLIQFC
                     RAGFAMIGRPMPYHMAEDVAAALGQLGLAYEKHEVPYDLIFDDTEANRLTILRFLFSE
                     HLTEMPRPAMLDLFTPFARGGQIQIHTACWHFSMGRPG"
     gene            7368..>8621
                     /locus_tag="VFV87_11480"
     CDS             7368..>8621
                     /locus_tag="VFV87_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5104427.1"
                     /translation="MLVSGLAAGLLSLAVAASASAQGGIDPASLAGLVQDEAAAKVEG
                     TWTKSVHTKPFLGEGYSYASGGEGQRVTFTLNVEREGDYQVLLSYTPGPNRTEKAAVL
                     IEAADGEKIVSVNQMRQPEGPYSFQPLGEFPLTAGPVKIVVSAEANKKGVVIADGVQI
                     LTPEQFKIAQEEAPKKPPKLLATLNIKPAKPAAKPDDKKPEEPASEEAPPFVRHAASK
                     PLTKITSARLDELMEEQVGGIENSIPIDDEAYIRRVTLDLIGRQPTLEESEAFLADQS
                     ADKRPKLVERLLASTEFGANWANYYSDVISYRTPEPELTFLNYTPFKVWLAEQFNADK
                     GWDETAYKILTATGKVADNPAATYVGFHQADKSRLASETTRVFLSTQIQCAECHDHKF
                     IDMPQETFHHVAAFFVRVQAKLPWND"
BASE COUNT         1629 a         2744 c         2778 g         1470 t
ORIGIN      
        1 ggcgttgacc gtggccaaat gacgccgcat gagggcttcg tcgaggacgt cgtcgtgaac
       61 gagcgtggcc gtgtggatca tctcaaccac ggtggccagc gtgagatgct cgtgcgtcac
      121 cttcccgccg accgccttag ccgccaaaag gagcagggcc ggccgcaggc gcttgccgcc
      181 cagcaggcag ccgtagccga cgagctcatc gacgtaggcg tgctcgctcc tcagctccga
      241 ctggagcagc gtttcgacct gagccaactc gtcggcgatg ggggcgtaga gcgactgcag
      301 cagggcggtg ccgcgagggg cggggcgggt gatcggcggc tgggtgagcg tgggcatcgg
      361 tgccattcac gaatcctgtt ggacgggcag gcgacacgcc tgccccactt tggcaggcga
      421 gacgcctgcc ccacggagtt acgtaaccgc aggcggcggc ggaacttcac gccgaaaatg
      481 gccgctcttg ccgccgtgtt tctcctcaag ctgaatctgc tcgatgacca tgccgcggtc
      541 gatcgccttg cacatatcgt aaatggttaa tgtggcggca cttacggcga ccagggcctc
      601 catctcgact ccggtccgcc cgacgatgcg aacctccgct tcaacgccga gcacgtcctc
      661 gccttcaaac gagaagctca ccgacactgc atccagcggc agcggatggc aaagcgggat
      721 cagatcgctg gtccgcttgg cggccatgat tcctgccagg cgggcgacct ccagcacgtc
      781 ccccttggcc aggccgcgat ctttgatccg ctgcaaggtc gcgggctgca tccgcacccg
      841 gccgctggcc cgggcccgcc gctcggtcgc cggcttgtcg ccgacatcga ccatccgcgt
      901 cgcgccggac tcgtcgaaat gggaaagatc ctctggcatg cggggattct cgcatgcgcg
      961 gcgaagtccg cctagacgcc agccgcttca gagcaagcgg ggaaagaaga ggggccgggg
     1021 gaaacggcgc gccgggaaac gcgccgcttc cataggtcga atcattcgac cgccccaaaa
     1081 gtcgcatccc gtattcgaag ccgccgcggg ggttcagccg cagcgggttg tggtttcgcg
     1141 taggtcccgt ccgctggacg ggacctggcc ggcgagtttg gaacgattgg cgattcaagg
     1201 ttccgcccgg cgggcagaac ctatcgcgga acgccacaga gggcgttcct tacaccaatt
     1261 ccttccgggc cccgatcagc gtccgcaggc gctcggccag cagcttggcg tcgaacggct
     1321 tcttgaacgt ttcgttgatc gccgagcggt caaagctgag cgggctgccg tcgtcgggca
     1381 acagggcgat caagatcgtc tcgccgaaat ccgggttctt gcgaaggttc tggcagatct
     1441 gcagggcctc gaccttgccg atcgagaagt ccgcaatgat gcaatccgga tggaagctct
     1501 cggcctgaat gccggcttcg aagccgctgg cggccacggc caccttgaat tgccgttcgg
     1561 ggggcagctc gcggcggaga ttctcgatca gaatctggtc ctgagcgacg atcagcacct
     1621 tggccatcga ctcgtcttcg aggtcgccca agggcatgcc gtgttctttg agaaacttga
     1681 tcagatactc gcggggaatg cgacggtctt gcgagccggg gatgcggtag cctttgaggc
     1741 gtcccgaatc gaaccacttg ctgaccgtgc ggggggccac tttacagatc ttcgcgacct
     1801 gtccagttgt gaagaccttc atcgcaggct ctccagttcg ctctcttgga ttcgttgcca
     1861 cgatcggtcg cggtgaggag ccgcttccga tcgcttgttg ccccggtctg cgtttcccgg
     1921 cgttctcgtt ccttctcgcc ggcacgtggg tcgttgccgg gccgtcgcaa acctctgggt
     1981 cggggcaaac gggttgtgct attggggtgg cttggtctca gttccctctg atccgcgcct
     2041 tacgaacaac atcggcaatc ggagcgttcg gctagcggcg gaatacgcgg aatgtgggca
     2101 gccttaacga gtgccccaag ctgcgtttcc ggacgcaccg actgccaaca ctcggcgttg
     2161 gcgattcagg gaagaaaccc gctatagagc ggctggcgcg gcttgggtcg gccgcgctgg
     2221 tcgctggcag gaagcggctg gcgccgctag atagctagaa tcccccctgg aattcgcgtg
     2281 tcgctcgttt gcgcggggcg ctggcgggcg acacacgtct tccgaatgtt agtttcgtat
     2341 gagcggggca agcgacttta gaaagtttga ccagtcatgc cgcgagaacg ggtcgattgg
     2401 ggcctaacgc atcgcaaacg agcgccgatt ggtgaacttg tgccgaatag tccgcccctt
     2461 ccgcagccgc gtatttggcc gtccggcgca gcaagccctc cttttgctac gatgaaatcc
     2521 gcagcttggg tcgaataatc gccacggccc gcgcatctag atctggatgg cacttgccgc
     2581 aatcactgat tcacgtctcc gcacaggaat gattcacatg acgctggttc ggggtggcat
     2641 cttgctcggc ttaatcgggc tggccttgct tggaatggca ggtgtctatc tggttgcggc
     2701 cgattcgacg ccgcaagcag cgaaacagaa gattcccctc gatgactggc cgcagtggcg
     2761 cggcgtcgat cgcagcggcg tttcgcccga ttcgggactg aacaccgact gggcggctcg
     2821 cccgccgaag ctcctgtgga cggccgaagg cctgggcggc ggctttgcca gcgtttctct
     2881 cgccagcggt gtgatttaca ccaccggcaa cttccccgac ggccaggccg tcgtcgcagt
     2941 cgatgccgag ggcggcaagc tgctctggaa gcagcccatc accgacgaac cccccagcca
     3001 ttccaagcaa ggctcgcgct ccacgcccag cattgacggc aagcacgtct atgccatcgc
     3061 ctccagcggc aaaatcgtct gcctgaacgc agccgatggc agcgaagtct ggtccaaaga
     3121 cttcaagagc gagtggggcg gccggatgat gtccggctgg ggattctcgg aatcgccgct
     3181 ggtcgatggc gactgggtcc tctgcacgcc cggcggcgcg gaagcgatga tcgtcgcgct
     3241 cgacaagctc accggcaagg aaatctggcg ctgcaaggcc cccgccttcg gcccaggcaa
     3301 ggaaggcgct ggttactcgt cgatcgtcat ctccaatgcc tgcggcgtga agcaatacgt
     3361 gcagctcgtc ggcaagggac tgatgggcgc gcgggccagc gacggcaaat tcctgtgggg
     3421 ctatagcgac atcgccaacc ggactgccaa ctgctccaca ccgctcatct cgggcgatta
     3481 catcttctgc tccaccggct atcgcacggg ggcggcactg gtcaagctcg tcaagtcggg
     3541 cgattcgatc gaggcccagg agcagtattt caagtcgggc gaagagttcc agaatcacca
     3601 cgggcagatg atcctgcaag acggatatgt ctatgccggc cacggccaca acaacggcct
     3661 gccgatctgc ctggaactcg attcgggcaa ggtcgtgtgg ggcggcacgg atcgcggtgt
     3721 gggaaccggc tcggcggcgg tcgcctgtgc cgatggccac ctcgtctttc gctatcaaag
     3781 cggcgaggtc gccctgatcg aagccacacc cagcgagttc cgcctgaaag gctcgttcaa
     3841 gccccagttc gtcggccgcg atccctgctg ggcccagccg gtcatcatcg gcggcaagct
     3901 gtacttgcgg gaccaggaca agctgatgtg ctacgacgcg aagtagccgc cgtagcacat
     3961 ctcgctccgc gagatgcgaa cgattctcta tgcgcgaaga ctgctgcgca tggaagatct
     4021 accgcgactc acggagcgag tcgcctacgg tgcgacgtgc ccttgatgaa ccggcgttca
     4081 accagcaaaa tgtcgggaca aacagcccgt ttgcgccggt caatcgcatt ctccgaagtc
     4141 ccgccatgat cttccagcac atcgacaacc tcaagcagca atatacggac aaatacgtcg
     4201 tcgtcgatga ctcgcagccg gagctgcagc gcttccgcgg tctgaccggc acggtcaaga
     4261 cggtcaatta cagcggcaag gcgctcgtcg agttcgacgg ctatgcgaac atcggctggt
     4321 acgacatcga tccgaccttt ctgaaggtcg tcgatgcccc gctgcccaag cccgaggaga
     4381 agccggccaa gaaagagaag gccgcacccg ccgccaagcc ggcagccgag aagccggcgg
     4441 cgaaggccgc gccggccaag cctgcgccag ccaagcccgc cgccgggccg acggccggca
     4501 aatctgtggc tgaaattctg gccgccgccc gcaagccagc cggcgcggtc gcggaagctc
     4561 cagctggcga gccgggggcg tcagcccccg gaggtgcggc tgttgcgccc gccaaagtcg
     4621 ctcctggcgg caagggcatg agcatggcgg acatactggc cgcagcccgc agcaaaggtg
     4681 gcgctgcccc cgtcacacca acccgcagcg taagcgagag cacgccggca gcgaaagctg
     4741 aagcccctgc tgccaaggcc gcagcatccg ccaagcccgc agccgcagcg cctgctgccg
     4801 ctgctccctc gaaggtcgct cctggtggca aggggatgag catggacgac atcctcgccc
     4861 tggcccgcgg caagaagccc gccgcggccg ctcccgccgc tccggcggca aactcggctc
     4921 ccgcttcaga gccgctccaa gaacccgagc ccgccagcga agaacctgct gcggaagaag
     4981 cggccgctcc ggccgccagc gctgccgagg aaagcgctcc ggcgggtggc ggcggctcga
     5041 agaaggacgt catcaccagc gtcgccgagc agatcgctta ctgccgcaaa gtggacgcaa
     5101 agtagggcgg gctctgccca ccacgtttcc atcgtgggct ctgcccacca cctttcgctc
     5161 ggcagttctt gatcgcactg cgaggtgggc acagcccacc ctacgcgctt ggagcaaaac
     5221 atgtccaacc cctccccgcc cccatcctcc ggcggctcaa gctggctgat catcctgctg
     5281 gtcatcctgg gtcttgtcat gctcacgtgc gccggcctgt gcggcggctg catctatctc
     5341 ggccagcgcg cggcggtcca cgtcggcaaa gctgccgaag agttcgccgg ctatttgcag
     5401 ctcatgcccg cgtttgccgc cacccagcaa gcggtggaca acgatccgca agtcgtccag
     5461 cgcctcggcc agccgatcga atcgctggcg atgcccaagc ggcagaacca aggcgacctg
     5521 aagcccggcg gcgagacatt cctctacgac attcgcggcc cccaggggac ggctatcgtc
     5581 agcggcgtcg ccacggctga atcgcccgcc ggcccttggc gagtctcgac gatcaacgtc
     5641 actttcgccg acggctccgt cgtcacggtc cccacccccg aaaatcaacc cgccggcggc
     5701 acgttcgaat tccaaaacac cgaaatccaa ctgccgcccg agctccacga gccgatcgaa
     5761 acgaagtaag cgcggcgtac ccaacaggcg ggtgcttctc tcagccaggt cttcccatcg
     5821 agaagtgcca gcacgccgtg tgaatttgga tttggccgcc gcgggcgaag ggcgtgaaca
     5881 ggtcaagcat tgccggccgc ggcatttccg tcaggtgctc ggagaacagg aaccgcagaa
     5941 tcgtcagccg gttggcctcg gtgtcgtcga agatgaggtc gtagggaacc tcgtgtttct
     6001 cgtacgcgag gccgagctgg ccgagtgccg cggcaacatc ttcggccatg tggtaaggca
     6061 tgggccggcc gatcatcgcg aagccggccc ggcagaactg aattagtgtg ttgtcgtttc
     6121 cggcgatggc ggtgagcagg cgtccgccgg gggcgaggcg ctggatgagg gctgcgagcg
     6181 tggccggcag gtcgggaacg taatagagca cgtggttggc gacgatcagg tcgaaatcgg
     6241 gctgctggat cgcggccagc gaattggcgt gctcgatggg cgaggcgctg aatcgcgcca
     6301 cgcgctctgc cgccagcgca ggcgaggcgg cgaccggctc gaccagcgcg agctgcaggc
     6361 gaggtggcgg gatttgcacc aatctcagca gacgctcggt gaacgttccc gtcccgcagc
     6421 cgaaatcgag cattctgagc ggctgccccg ccgcaacgag cggcgcgatg acgcgggcat
     6481 aagccgcggc gtcgttcacg gcttccgtgg cacgggcctc gaaaaaggcg tagtcgctga
     6541 ggatcggggc gaagtctttg gtggtgtcca tgccagtggg cgtaaagagt tggccggctg
     6601 tgggttggag cgatttccag gaattttggc gacagtagtg aataaacgta cccgttcgcg
     6661 catctaatga tgttgggctg cgcgacgccg cgcataccgg gtgggcgatt atgcataagt
     6721 tgcatgaatt gcataactac cggctcggtg cttgcgacgc aacatgtttc tccacaagca
     6781 cttacgaaca tttctacgtc aggatgtttt ttgagccgaa gatgcataga cgcgaacagg
     6841 ggtcaacgaa cgccagcgag cttcgaggcg aaggctccgg gtgggttacg gaacaaagac
     6901 tccccgcgca gggcctgttc accaattcgg ggagctggcg cgaaacggct caacccggcg
     6961 caacgaaagc gcaacagcgc aacatcctca accggggggt tgcgcgttgc gcaagcgatt
     7021 gtgccgcagc ggcttgcggc gaattcgcgc aattgcgttg cgcggcaaaa aagtcgcgaa
     7081 gttgcgcgtt gccatggtaa cccgacgcgc aagcaaggtt gggatccgac ctgttcagac
     7141 tcactggttg gtgcaagtcg ggaggatact ccgcgatgcg catcggccct ggcacatgga
     7201 gcgccgtctg ccactcgcgg gagcgaggag cctacgatgc ggcgagaagc gtttgacaac
     7261 cgccggctga ggcgctaagc tcaacgctgg caccttgcac aactgaccga acctctagct
     7321 gccaatggag ttgcgacgtg atgcgaaggc tccgaattaa ctgcccgatg ctcgtctctg
     7381 gcctggccgc gggattgcta tcgctggcgg ttgctgcctc ggcaagcgcc cagggtggca
     7441 tcgacccggc ttctctggcg ggactggtgc aggacgaggc ggcggcgaag gtggaaggga
     7501 cctggacgaa gtcggtccac accaagccgt tcctgggcga gggctatagc tacgcttccg
     7561 gcggcgaggg ccagcgggtc acgttcacgc tgaatgtgga acgagagggc gactaccagg
     7621 tgctcttgtc gtacacaccg ggaccgaacc gcaccgagaa agccgccgtg ctgatcgagg
     7681 cggctgacgg cgagaaaatc gtcagcgtca accagatgcg ccagcccgag gggccgtatt
     7741 cgttccagcc gctgggcgag tttccgctga cggccggccc ggtaaagatc gtcgtctcgg
     7801 cggaagcgaa caagaagggc gttgtgatcg ccgacggcgt gcagattctc acgcccgagc
     7861 agttcaaaat cgcccaggaa gaagccccca agaagccccc gaagctcctg gcgacgctga
     7921 acatcaagcc ggccaagccc gccgccaagc cagatgataa aaagcccgaa gagccggcct
     7981 cggaggaggc cccgccgttc gtccgccacg cagcgagcaa gccgctgaca aagatcacat
     8041 ccgcccggct tgatgagctg atggaagagc aagtcggcgg catcgagaac tcgattccga
     8101 tcgatgacga agcgtatatc cgccgggtca cgctcgacct cattggccgc cagcccacgc
     8161 tggaggagag tgaggcattc ctggcggacc agtccgccga caagcggccc aagctggttg
     8221 agcggctgct ggcgagcacg gagttcggcg ccaactgggc gaattactac agcgacgtca
     8281 tcagctatcg cactcctgag cccgagctga cattcctgaa ctatacgccg ttcaaggtgt
     8341 ggctggccga gcagttcaat gccgacaagg gctgggacga aacggcgtac aagattctca
     8401 ccgcgaccgg caaggtcgcc gataaccccg cggccaccta cgtcggcttc caccaggcgg
     8461 ataaatcgcg gctggcgtcg gagacgacgc gcgtcttcct ttcgacgcag atccagtgcg
     8521 ccgagtgcca cgaccacaag ttcatcgaca tgccgcagga gacgtttcat cacgtcgcgg
     8581 cgttcttcgt ccgcgtgcag gccaagctcc cttggaacga c
//
LOCUS       DASUGB010000600         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_761933, whole genome shotgun sequence.
ACCESSION   DASUGB010000600 DASUGB010000000
VERSION     DASUGB010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761933"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFV87_11485"
     CDS             <1..385
                     /locus_tag="VFV87_11485"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5104428.1"
                     /translation="AAKMRQMFAKIRELRAKYPDEADYSRERKRWELENPIDPGTIHD
                     VVDHIDHIVKVAGTSHVGIGSDFDGISTCPKQLEDVSTYPLITQELLNRGYQPDDINQ
                     IMSGNIFRVMQRAEEVAAELQAAAN"
     gene            390..1319
                     /locus_tag="VFV87_11490"
     CDS             390..1319
                     /locus_tag="VFV87_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104429.1"
                     /translation="MHKLKSQFPTFLASGRLVADIVSSEWVALLGGVAICVLGVFLIG
                     FVAGMVGDSNGRFMSLLKIWSCSAIAVMGAWTSLGSGAWHWRFLAGIWASLCVLAAFI
                     SVCDAPAFGIRAFPERFPVQAALIYACSAFGAQWLGSVANVRILRQPRPAVPNSLRAG
                     QFNIAALLLIMAIVAAFLGMIRIVCDLLLRWQPRVHQLVDGDIWVWVLYSSTLALVAV
                     AATLRSGWKLALLVLGVLIALWIASHWDQSISRSASRSQFVRSIAYLMWPVAPTLLIA
                     STIQWLWLRVCGFRLLPSDWFSVHLRTQHIIRS"
     gene            1330..2439
                     /locus_tag="VFV87_11495"
     CDS             1330..2439
                     /locus_tag="VFV87_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104430.1"
                     /translation="MNNPEQESPPAVELAGVSKRFGERVILDRIDLEIARGEVLVLVG
                     PSGSGKSTLLKIVSGIETPDEGRVKLAGVDCTEEPPYRRAVHTVFQNYALFPHLDVAG
                     NVAFPLAVAGIPRSKREPLVMKALAWVRMDQHARRRIDALSGGEKQRVALARALVDQP
                     QCVLLDEPLSALDPHLRSGTLDLLTRLQGELGLTYLFITHDREEALRTGHRIGVLNHG
                     RLEQVASPEEIYQRPATAFVASFLGKINWLAGEVFHDGGRPFVQTCGCRVPLVSPVLS
                     GAVQVGLRPEDVRIRTPTRSVSEGVSATVVNRLFFGDSTTLTVRTVGGSELTIDQRPA
                     SDAQPGVAVQIEWSPEAMHIFPAGEEVATGKEAAK"
     gene            2436..3218
                     /locus_tag="VFV87_11500"
     CDS             2436..3218
                     /locus_tag="VFV87_11500"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104431.1"
                     /translation="MKTKTTRLFALPAVIYLAVLFLGPTSVVLAYSFLERDIYGGVLP
                     RISWEAWRMATDWITLRILGRTVLLAGGVTALNLLLAYPTAAALARLPRERRELWVLI
                     LGFPMLTSLLLRTYGWLNVLPLQWRGTLWAVALVEACNYLPFMVLPLLKAYERADRTL
                     VLAAMDLGATPWQAFWRVTFPITLSGAGAGSALVFIPVSGEYLVPHFIGEGKVSVAGT
                     TVMEWFEHRHWPYAAAIAAWLAAIVLVPIGAGLWWKSREPAL"
     gene            3300..4100
                     /locus_tag="VFV87_11505"
     CDS             3300..4100
                     /locus_tag="VFV87_11505"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104432.1"
                     /translation="MNHRTEQLAIRWLGRAALAVVLLGMYLPVVMTFIYSFNASRIGT
                     VWTGFSLNGYLELWHEEPLWQGLEASLLIGAAASTLSVLAGTLAALGLSAWRPRTKLL
                     AQGVLALPLVTPEVILGLSLAMFFSALHVQQGYGTVVAAHCVFGISYAFVVMSGPVQD
                     FDQNLYSAALDCGATPWQAYSRVIVPILAPSLVVAWLFVFALSFDDFLITFLTKGPGA
                     DTLPIKIYSQMRFGIKPLTSALFVILFVVTLSGALVAQRLMRRSYAAE"
     gene            4155..>4544
                     /locus_tag="VFV87_11510"
     CDS             4155..>4544
                     /locus_tag="VFV87_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104433.1"
                     /translation="MPQLTPDPFTVAMPENHAMRTARPRYNRRTWLQVTGAGLAAGAL
                     SGCEGRSASSKRVLKVFNWSLYIDDKVIPEFEQQAGCRVVYDNYSSDSELEARLATGG
                     GAYDVVFPSDRAMQALLAKQLLQPLDQA"
BASE COUNT          722 a         1432 c         1508 g          882 t
ORIGIN      
        1 cgcggccaaa atgcggcaga tgttcgccaa aatccgcgag ctgcgggcca aataccccga
       61 cgaagccgac tacagccgcg agcgcaagcg gtgggagctg gagaacccga tcgatccggg
      121 cacgatccac gacgtggtcg atcacatcga ccatatcgtc aaggtcgccg gcaccagcca
      181 cgtcggcatc ggcagcgact tcgacggcat ttccacctgc cccaagcagc tcgaagacgt
      241 ctcgacgtat ccgctcatca cccaggagct cctcaaccgc ggctaccagc cggacgatat
      301 caaccagatc atgagcggca acatcttccg cgtgatgcag cgggcggagg aagttgccgc
      361 ggagctgcag gcagcggcga attgagccca tgcacaagct gaagagccag tttccgacct
      421 ttctcgcgag cggccgcctg gtcgcggaca ttgtctcgtc ggaatgggtt gccctgcttg
      481 gcggggtagc gatctgcgtg ctcggcgtct tcctgattgg gttcgtagcg ggaatggtgg
      541 gagactccaa tggccggttc atgtccctgc tgaagatctg gtcctgctcg gcgatcgccg
      601 tgatgggcgc gtggacttcg ctcggcagcg gcgcgtggca ctggcgattc ctcgctggca
      661 tctgggcttc gctgtgtgtc ctggccgcct tcatcagcgt ttgcgatgcc ccggcgtttg
      721 ggatccgagc atttccagag cgatttccag tgcaagctgc actgatctat gcctgttctg
      781 cttttggtgc ccagtggctc ggatcggtgg cgaatgtgag aatcctccgc cagccccggc
      841 ctgcagtccc aaactctctc cgcgccggcc agttcaacat tgcagcactg ttgctcatca
      901 tggccattgt cgcggcgttc ctcggaatga tcaggatcgt ctgcgacttg ctgcttcgtt
      961 ggcagccgag ggttcatcag ttggtcgatg gcgatatatg ggtgtgggtc ctgtattcga
     1021 gcacgctggc cttggtcgcg gtggcggcca cgctccgatc gggatggaaa ctggcgctgc
     1081 tggtgctcgg cgttctgatc gccctatgga ttgccagcca ctgggaccaa tccatctccc
     1141 gctctgcgag ccgatcgcag tttgttcgct ccatcgctta cttaatgtgg ccggtcgctc
     1201 caacgctgct gattgcgtcc acgatccaat ggctgtggct gcgcgtgtgc ggatttcgtt
     1261 tgctgccgag cgattggttc agcgtccatc tgcggacgca acacatcatc aggagctaac
     1321 cccactgaag tgaacaaccc agagcaagaa tctccgccgg cggtggagct ggcgggcgtg
     1381 agcaagcggt ttggggagcg agtgatcctg gaccggatcg acctggagat cgcccggggc
     1441 gaggtgctgg tcctcgtcgg accgagcggc agcgggaaga gcacgctgct gaagatcgtg
     1501 tcgggcattg agacgcccga cgagggccgt gtgaagctgg ccggcgtgga ttgcacggag
     1561 gagccgccgt atcgccgggc ggtccacacc gtctttcaga actacgccct ctttccgcat
     1621 ctggatgtgg cgggcaacgt cgcttttccg ctggcggtgg cgggaattcc gcggtcaaag
     1681 cgggagccgc tggtgatgaa ggcgctggcc tgggtgcgga tggatcagca cgcccggcgg
     1741 cggatcgacg ccctctccgg cggcgagaag cagcgcgtcg ccctggcccg ggctttagtc
     1801 gatcagccgc aatgcgtgct cctcgatgag ccgctctcgg ccctcgatcc gcatctgcgc
     1861 tcgggcacgc tcgacctgct gacgcgactg caaggcgaac tcggcctgac gtacctgttc
     1921 atcacgcacg accgcgagga ggccctgcgg accggccacc ggatcggcgt gctcaaccac
     1981 ggccgcttgg agcaggtcgc gtcgccggag gagatttacc agcggccggc gaccgctttc
     2041 gtcgcgtcgt ttctcggcaa gatcaactgg ctggccggcg aagtgtttca cgacggtggc
     2101 cggccgttcg tccaaacctg cggctgccgc gtgccgctgg tttcgccggt ccttagcggc
     2161 gccgtgcagg tcggcctgcg gccggaggac gtgcggatcc gcacaccaac ccgaagcgtc
     2221 agcgagggag tgtctgccac tgtcgtcaat cgcctcttct tcggcgattc gacgacgctg
     2281 acggtgagga ccgtcggcgg cagcgagctg accatcgacc agcggccggc cagcgacgcg
     2341 cagccgggcg tagcggtgca gatcgaatgg tcacccgagg cgatgcacat cttcccggcg
     2401 ggcgaagaag tcgccacggg aaaggaggcc gcgaagtgaa gacgaagacg acgcgcctgt
     2461 tcgcgctgcc ggcggtgatc tacctcgcgg tcctgttcct ggggccgacg agcgtggtcc
     2521 tggcctattc cttcctggag cgcgacatct acggcggcgt tctcccacgc atctcctggg
     2581 aagcgtggcg gatggcgacc gactggatca cgctccgcat cctcggccgc acggtgctcc
     2641 tggccggcgg cgtgacggcg ctgaatctac tgctggcgta tccgaccgcg gcggccctgg
     2701 cccggttgcc gcgcgagcgg cgcgagctgt gggtgctcat tctcggcttt ccgatgctca
     2761 cgtcgctgct gctgcggacc tacggctggc tgaacgtgct gccgcttcag tggcgcggga
     2821 cgttgtgggc ggttgccctc gtcgaggcct gcaactatct cccgttcatg gtgctgcccc
     2881 tccttaaggc ctatgaacgg gccgaccgga cgctcgttct cgcggcaatg gacctgggag
     2941 cgacgccgtg gcaggcgttc tggcgggtca cgtttccaat cacgctcagc ggcgcaggcg
     3001 ccggctcggc tctcgtgttc attcccgtca gcggcgagta cctcgtgccg cacttcatcg
     3061 gcgaagggaa ggtgagcgtc gccgggacga ccgtgatgga atggttcgag catcgccact
     3121 ggccgtatgc cgcggcgatc gctgcctggc tggcggcgat cgtgctcgtg ccgattggcg
     3181 cgggcctgtg gtggaaatcg cgcgagcccg cgctgtaggg aacgccctcc gtggcgttcc
     3241 gcgaactgac aatggagacc gcattcggaa cggcacggag gccgttccct acagaacgta
     3301 tgaatcaccg cacagaacaa ctggcgatcc gctggctggg ccgggcggcc ttggccgtcg
     3361 tgctcctggg catgtacttg ccggtggtga tgacgttcat ctattcgttc aatgcctcgc
     3421 ggatcggcac cgtctggacc ggcttctcgc tcaacggcta cctcgagctc tggcacgaag
     3481 agccgctctg gcaagggctg gaggccagcc tgctgatcgg ggcggcggcg agcacgctct
     3541 cggtcctggc gggaacgctg gcggcgctcg gcctgtcggc ctggaggccg cggacaaagc
     3601 tgctggccca gggggtgctg gccctgccgc tggtcacgcc ggaagtcatc ctcggcctgt
     3661 cgctggccat gttcttcagc gcgctgcacg tccagcaggg ctacggcacg gtcgtcgccg
     3721 cccactgcgt atttggaatc tcctacgcct ttgtcgtaat gagcgggcct gtgcaggatt
     3781 tcgaccagaa cctgtactcc gcggccctcg actgcggcgc gacgccgtgg caagcctatt
     3841 cgcgggtcat cgtgccgatc ctcgccccca gcctggtcgt cgcgtggctg ttcgtcttcg
     3901 ccctctcgtt cgatgatttc ctcatcacgt ttttgacgaa aggacccggc gccgatacgc
     3961 tgccaatcaa gatttattcg cagatgcggt tcggcatcaa gccgctgacc agcgccctgt
     4021 tcgtaatcct gttcgtggtg acgctgagcg gagcgctagt cgcccagcgc ttgatgcggc
     4081 ggagctatgc ggccgagtaa cctcgagggg gtcgggagtc aattgcggcc aagattatcc
     4141 atgtggaaac gtccgtgccg caattgactc ccgatccctt cacggtggcc atgccggaga
     4201 accatgcgat gcgaactgca cgaccgaggt acaatcgccg cacgtggctg caagtcacgg
     4261 gcgctggact ggctgccggc gctctcagcg gctgcgaggg cagaagcgcg agcagcaaac
     4321 gagtgctcaa ggttttcaat tggagcctct atatcgatga caaggtgatt cctgagtttg
     4381 aacagcaagc cggctgccgc gtggtctatg acaactactc cagcgattcg gaactggaag
     4441 cccggctggc gacgggcggt ggcgcgtacg atgtggtttt tccctcggac cgagccatgc
     4501 aggcgctcct ggccaagcag ctcctgcagc cgctcgatca ggcc
//
LOCUS       DASUGB010000601         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_622198, whole genome shotgun sequence.
ACCESSION   DASUGB010000601 DASUGB010000000
VERSION     DASUGB010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622198"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1792)
                     /locus_tag="VFV87_11515"
     CDS             complement(38..1792)
                     /locus_tag="VFV87_11515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5104434.1"
                     /translation="MKRRLPASTDLTPATVADTAVAAELIQRRELALLALILLAGVSL
                     RIFALSRSTVEHFDEGVYASNLYFGPPDYAYPLQRFYAPPLLPALIETGMIAGLPPNL
                     AALLPGFLAGCGTIVALWWFGRSWFSPAVGLAAATLAALSEFHITYSPTALTDVLLGL
                     WIVLAVDAIARSLAGEDFRWAIAAGIYTGLAWWTKYNGWLPLAIEAAALPLLWLVTQR
                     SIVRKLGCFVTTVAVAVVVWLPYYLSLASTGGYGPIAANHANYVVGFAGWLDSASRQI
                     AAQHVMEGPLSAAALGIAAVALWIAASNGHRPAMPWMAGLAAAIAMALALRFTSFVVL
                     GLLGGAGLAWSLIVAWSARTSGTVNVRSAVGTCLVAAWWLGLLVATPCYWPYPRLVLP
                     WLMASWLGGALFCQQCVQLANRSQKTPATQPWSGLAIVVAGLAGIMLLGSSLSPALQA
                     PSRPDAASYQRIAAEIHAGSGLPEIRAIYTCGEPALLFQLRAAGEPLVAPSRDIPHEA
                     VTIDGAAIATLLILGPHARGDLPCQQQWAERKNRWELIASYDYMPRHIVRLDLYDPRQ
                     PPALDQSALNRIEVYRLK"
     gene            1879..3270
                     /gene="asnS"
                     /locus_tag="VFV87_11520"
     CDS             1879..3270
                     /gene="asnS"
                     /locus_tag="VFV87_11520"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX5104435.1"
                     /translation="MQRISVKDARQSAAIGHNVLVQGWVRTRRDSKGGFSFLEVNDGS
                     CLANLQVIADAQLPNYESEAKHLVAGCSVSIEGEVKASGGKGQVTEVLAKKLTVHGWA
                     DPEAYPLQKKQHSLEKLREWAHLRPRTNTFGAVTRVRNCVSRSIHNFYQEEGFLYIHT
                     PIITASDCEGAGAMFRVTTLNLEKLPREGTKINHGLDFFGRPTYLTVSGQLDAEIFAC
                     SLGKVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFFDLNDNMDLAERFLKRIFADVLA
                     QCPDDMALFNEHYDKTAIETLEKIVASDFRRLPYTDAVEILQHCGEKFEFPVQWGVDL
                     QSEHERYLTEKHFQGPVILHDYPRTLKPFYMRVNDDGKTVRAMDVLVPKVGEIIGGSQ
                     REERLDVLEQRMAEQHLNAEDYWWYLDLRRFGTVPHAGFGLGLERAVQFITGMGNIRD
                     VIPFPRTPGSAEF"
     gene            3311..>3509
                     /locus_tag="VFV87_11525"
     CDS             3311..>3509
                     /locus_tag="VFV87_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5104436.1"
                     /translation="MKLKDVKIAGKSLVLAKRAEVDALESDLWITLPKGYRDYVTQLG
                     QGSLGSFVRVYPPWQIAKELTE"
BASE COUNT          729 a         1082 c         1148 g          550 t
ORIGIN      
        1 tgggcgattc tacgcgcccg gcgcgggcag ccgcaaccta cttcaggcga taaacttcga
       61 tccgattgag tgcgctctga tctagcgcag gaggttgccg cggatcgtac agatcgagcc
      121 gcacgatgtg tcgcggcatg tagtcgtagg aggcgatcaa ctcccaacga tttttgcgct
      181 cggcccattg ttgctggcag ggcagatcgc cgcgggcgtg cggaccgaga atgagcagcg
      241 tcgcaatcgc cgcgccatcg atcgtcaccg cttcgtgggg aatgtcgcgc gatggcgcca
      301 caagcggctc ccccgcagct cgcagttgaa acagcagggc cggctcgccg caggtgtaaa
      361 tggcgcgaat ttccgggaga ccggacccgg cgtgtatctc ggcggcgatt cgctgataac
      421 ttgccgcgtc cggccgggag ggcgcttgca gcgcggggct aagactcgaa cccaatagca
      481 tgatgccggc caggccagcg acaacgatcg ccaagccgct ccacggctgc gtggcggggg
      541 ttttctggct gcgattggcc agttgcacgc actgctggca gaacagggcg ccgccgagcc
      601 acgacgccat cagccagggg aggaccaaac gcggatatgg ccagtagcac ggtgttgcca
      661 cgagcaggcc cagccaccac gcggcaacca ggcacgttcc aaccgctgac cggacattca
      721 ccgtgccgga cgtgcgagcg gaccaggcga cgatcagcga ccaggcgaga cccgcgccgc
      781 ccagcaagcc cagcacgaca aagctcgtga aacgcagcgc gagtgccatt gcgatcgcgg
      841 cggccagccc ggccatccac ggcatcgctg gtcgatggcc attgctggcc gcgatccata
      901 gggccaccgc ggcgattcct agtgcagcgg cactcagcgg tccctccatg acgtgctggg
      961 cggcaatctg ccgactcgcg gaatccagcc aaccggcaaa gcccacgaca tagttggcgt
     1021 gattggcagc aatcgggcca tagccgccgg tggaggccag cgaaaggtag tagggcaacc
     1081 acacgaccac cgcaacggcc accgtggtaa cgaagcaacc aagcttgcga acgatcgacc
     1141 gttgtgtgac cagccagagc agcggcaggg cggcggcttc gatggccaaa ggcaaccagc
     1201 cgttgtactt ggtccaccag gccagaccgg tatagattcc cgcggcgatc gcccagcgga
     1261 agtcttcgcc ggcaagtgag cgggcgatgg catcgacggc gaggacgatc cacaggccga
     1321 ggagcacgtc cgtcagcgcc gtcgggctat aggtgatgtg aaactcggat agcgcagcca
     1381 gcgtcgccgc ggcgagaccg accgccgggc tgaaccagct tcgaccgaac caccacaggg
     1441 caacaatcgt cccacagccg gccaagaatc ctggcaacag cgcggcgagg ttcggcggca
     1501 gaccggcaat catgccggtc tcgatcagcg cgggcaacag cggaggcgca tagaatcgct
     1561 gcagcggata ggcatagtcc ggcggcccga aatagagatt ggaggcatag accccttcgt
     1621 cgaagtgctc gaccgtggaa cgcgagagcg cgaaaatccg gagcgacaca cccgccagga
     1681 gaatcagtgc gagcaatgcc aattcgcgtc gctggatgag ctctgccgca accgctgtgt
     1741 cggcgacagt cgcaggcgta aggtctgtcg atgcgggcag gcggcgtttc atcggcaggg
     1801 catactccca tttcccagcg caacttcagc cgccagaatg atcgctgcca agcaattcca
     1861 caaggaagga cgagggcgat gcagcgaatc tcggtgaagg acgcccggca gtcggcggcg
     1921 attgggcata acgtgctcgt gcagggctgg gttcgcacgc gccgcgacag caaaggcggc
     1981 ttcagctttc tcgaggtcaa cgacggctcg tgcctggcga atctgcaggt gatcgccgac
     2041 gcccaattgc cgaactacga atccgaggcg aagcatctcg tcgccggctg cagcgtttcg
     2101 atcgaagggg aggtcaaagc gtcgggcggc aaggggcagg tgaccgaagt gctggcgaag
     2161 aagctcaccg tacacggctg ggccgatccg gaggcctatc cgctgcagaa gaagcagcat
     2221 tcgctggaaa agctccgcga atgggcccat ctgcggccgc ggacgaacac atttggggcc
     2281 gtcacgcgcg tccgcaactg cgtcagccgc agcatccaca acttctatca ggaagagggc
     2341 ttcctctata tccacacgcc gatcatcacg gccagcgact gcgaaggggc cggggcgatg
     2401 ttccgcgtga cgacgctcaa tctggagaag ctgccccgcg agggaacgaa gatcaaccac
     2461 ggcctcgatt tcttcggccg cccgacgtac ctgaccgtca gcggccagct cgacgcggaa
     2521 atcttcgcct gctcgctggg caaggtctat acgttcgggc cgacgttccg ggccgagaac
     2581 agcaacacct cgcggcacct ggccgagttc tggatggtcg agccggagat ggcgtttttc
     2641 gacctgaatg ataacatgga cctggccgag cggttcctca agcggatttt tgccgacgtg
     2701 ctggcccagt gcccggacga catggccctg ttcaacgagc actacgacaa gacggccatc
     2761 gagacgctgg aaaaaatcgt cgccagcgac ttccgccgcc tcccctacac cgacgcggtc
     2821 gaaatcctgc agcactgcgg cgagaagttc gagtttcccg tgcagtgggg ggtcgatctg
     2881 cagtcggagc acgagcgcta cctgaccgag aagcattttc aggggccggt gatcctgcac
     2941 gactatccgc ggacgctcaa gccgttctat atgcgggtca acgacgacgg aaagaccgtg
     3001 cgggcgatgg atgtgttggt gccgaaggtc ggcgagatca tcggcggcag ccagcgcgaa
     3061 gagcggctgg acgtgctcga gcagcggatg gccgagcagc acctgaacgc cgaggattac
     3121 tggtggtatc tcgacctccg ccgcttcggc accgtgccgc acgccggctt cggcctgggc
     3181 ctggagcggg ccgtgcaatt catcaccggc atggggaaca tccgcgacgt gattccgttc
     3241 ccgcggacgc cggggagtgc cgagttttag gggaacgatt ctcaatccac aattaacaat
     3301 ggagaattcc ttgaaactca aggatgtcaa gatcgccggc aagtcgctcg ttcttgcgaa
     3361 gcgagccgaa gttgatgcgc tcgaaagcga tctgtggatc acgttgccca agggctaccg
     3421 ggattacgtg acgcaactgg gccagggctc gctgggttcg ttcgtgcggg tctatccacc
     3481 gtggcaaatc gcgaaggagt tgactgagt
//
LOCUS       DASUGB010000602         7924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_66403,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000602 DASUGB010000000
VERSION     DASUGB010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66403"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFV87_11530"
     CDS             complement(<1..820)
                     /locus_tag="VFV87_11530"
                     /inference="COORDINATES: protein motif:HMM:NF039748.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104437.1"
                     /translation="MILDQEEIRDYFAPAAGSFWRWEENGRVLAWNDGVTIAYREFLE
                     GVLARLAPHGLPPLGSVLLLLAACRDNWTEAPSRRVLLEAELAASGGQDFEEFAADVL
                     NKLTEVNWHRHRFRAETGGTAGLAEAIFEDAPGRYPREQSLALLEHLRRGLTEGDRQG
                     QSESALEELLHSLGCLRWGLMRFDPQSVQLRLQTGLDDLPRKAPVEPPPPSSARDLIT
                     ALQDDPDLGAVARLAQLLLAAVHLPRAISDPDALPVGGVSDISNRGPLDRLLLSE"
     gene            complement(817..1578)
                     /locus_tag="VFV87_11535"
     CDS             complement(817..1578)
                     /locus_tag="VFV87_11535"
                     /inference="COORDINATES: protein motif:HMM:NF039751.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104438.1"
                     /translation="MNVRWALLVPRESAAGLARLRRIPALAVSEAGEGIWLRGNALDE
                     SLERELKRIPGGERFAALDDGQLVPEGCLVPLGRLPGEGWVLLADWLQVSLPMVKLVE
                     RKNVVVRSANERPFAERKATNLSLVPTGDAREPTLLETSLSSLTQYVATAPQWRIERW
                     TFAATADGRAIVRGTPLAPIPGTQWVEVEGVATPAGFTWSPPVDAEVVRQVLQLAKDE
                     VALLRPDGTWDRIAPDQWVRASRSALRGTQEALLR"
     gene            complement(1575..2741)
                     /locus_tag="VFV87_11540"
     CDS             complement(1575..2741)
                     /locus_tag="VFV87_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5104439.1"
                     /translation="MHPPPDDCRLALARRLNAEVLASLKAAFVGKDEIIDLLGVCLAG
                     GENLFLLGPPGTAKSALVQQLARRIDGRVFDYLLTRFTEPNELFGPFDIRKLRDGELV
                     TNTEGMLPEAALVFLDELLNANSAILNSLLMVLNERVFRRGRETRRLPLLMVVGASNR
                     LPEDDALAALFDRFLLRVRCDNVPADSLGDVLAAGWKLDQGLAEAVPTLAVDDIRQVQ
                     SLVAEVDLAPVRTTYIDLVHKLRQAGIPVSDRRAVKLQRLVAASAALCGRAAANTTDL
                     WILRYIWDVDEQREVIAAIVNQAVEASPAVELEGSHPRARQSDGPDPEELAKDLKRIG
                     ERLAEAALAEAERAVLRDQLGLLSGRCQWVVNAQQREFLDQRVAELWQQLGVQP"
     gene            complement(2794..3444)
                     /locus_tag="VFV87_11545"
     CDS             complement(2794..3444)
                     /locus_tag="VFV87_11545"
                     /inference="COORDINATES: protein motif:HMM:NF039424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104440.1"
                     /translation="MKLDAFLAALFDHGRVQVLRPQAAITPADEKVALVKLQNQAAVL
                     AAEFPGQPPPLFPLAALWAATSFYRACQLAVYRDLDAQAIEELLGAVCPAGERAARHG
                     SVDIVFRYLPDLVRHASAASQQDPLVAKLRQWAADWPLSSVGMAATSPRHEDDFTSHP
                     GLVQLYVDRILAKKDWSRLAQPMVAEAVRRSLGAHGGLWPDAAPTGSGVNCDHRCP"
     gene            complement(3441..4214)
                     /locus_tag="VFV87_11550"
     CDS             complement(3441..4214)
                     /locus_tag="VFV87_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104441.1"
                     /translation="MISWNSFHAHTTPIIAACLLPGWVAAADISRLAAGLESEQFAER
                     ELAERELLELGLAVVDESLNSAVLPENATDADADQHFTKLGGVVRALLQEKLYVHLDR
                     LQGLESQFRASRVRALIEQHFEHRLGGAQVGFAKRLPPAEAEKVHGYSGGFREGTTWF
                     DTQFGNRSTFTVTSIRILVRLTDKQTGKKIERQATLGSDGAPLLPGQTVNWSVDLGIK
                     RTSDDEFFWETVAIYGTAPVVEDVSGAIPVPPAIPRIKK"
     gene            complement(4211..5143)
                     /gene="ispH"
                     /locus_tag="VFV87_11555"
                     /gene_synonym="lytB"
     CDS             complement(4211..5143)
                     /gene="ispH"
                     /locus_tag="VFV87_11555"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5104442.1"
                     /translation="MKILLAAPRGFCAGVNMAIESLDLAIRAFGTPIYVYHEIVHNKY
                     VVETFQAKGATFVDSLEEVPEGSTLLFSAHGVSPEIRRIARERKLRAIDATCPLVTKV
                     HLEAIRFAKEGYTILLIGHEGHDEVIGTMGEAPQAIVLVESVEHADRLEFPSGTKLAY
                     LTQTTLSVDDANRIIDRLKGRFPQVVGPPKEDICYATQNRQEAIRLLAREADAVLVVG
                     SQNSSNSQRLKEISLELGVPAYLVDGPNDIDDDWLTRASTIVLTAGASAPESIVEECI
                     GRLRQTHHATVEIRTIRSEDVHFPLPRELRTISV"
     gene            complement(5293..5883)
                     /locus_tag="VFV87_11560"
     CDS             complement(5293..5883)
                     /locus_tag="VFV87_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104443.1"
                     /translation="MSELSSLADHPRIVVAGTSCCGKTTFSRQLAQRLGRRHIELDAL
                     YWGPNWTPQPDFVSRVDEATAAEEWIVDGNYTMVRELTWARATALVWLNLGLPLVFWR
                     SLTRTCQRIGTGEAICGSNRETVAKSFFHWDGIPWWVLRTFRSRQRELRSLLPGPAFA
                     HLHVIELRQPAEADALLAGVRELVEQNGVTVAETAK"
     gene            complement(5876..7591)
                     /locus_tag="VFV87_11565"
     CDS             complement(5876..7591)
                     /locus_tag="VFV87_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104444.1"
                     /translation="MDSTTQSPSPHQAADAPAGGGAAPAAAALAAEVHADKTAKTAGL
                     EQTWLRFETVLDRVGDWLNPILVKEARQAMKSRQFVVTFSLLLIFGWLWTVIFIALSV
                     PDVFFAPVGPAVLTGYYIVLSIPLLIVVPYAAFRSLAGECEDGTYELLSITSLSARQI
                     VLGKLGSAVLQMMVYYSALAPCIAFTYLLRGIDVLTIGMFLTYTFLASLLLSIVGLML
                     ATVTRARHWQVLLSVVFVMALLVFTVIWDFFIIAVIVNRQTPPFDEADFWIANLCGLS
                     FYLPIAALLLQIGAGQITFASENRSGPLRVLLAGLHVLIVAWFTYYWLRTDTGELLFG
                     QIIVAAGFWAVIGAFLTGETAQLSPRAKRQLPQSFLGRMAFTWFNPGSGTGYVFAMLN
                     LLSVLAIAWIGVSAALIGGFEYYPALPEYYYFSATLVGYVLGYLGLVRLMIVALRRVT
                     HVGLLATFLCQVIAASGAVLFPYLMHVVSVWGDFSNSSYSLVQLPNWVWTLYEVHEQT
                     VFSGWELTATVASAGLVIFLVNLVVAAREVEHVRAAAPQRVIEDELAQHPELSPRKKK
                     NPWDE"
BASE COUNT         1484 a         2618 c         2528 g         1294 t
ORIGIN      
        1 gctcgctgag cagcagccgg tcgagcgggc cgcgattcga gatatcgctc acgccgccga
       61 ccggcagggc gtcgggatcg ctgattgccc gcggcagatg cacggcggcc aacaggagct
      121 gcgccagccg cgcgaccgcg cccaggtcgg ggtcgtcttg cagggccgtg atcagatcgc
      181 gggccgatga gggcggcggc ggctcgaccg gcgcttttcg cggcaaatcg tcgagacccg
      241 tctgcaggcg aagctgcacc gactgcggat cgaaccgcat cagcccccag cgcaggcagc
      301 ccaggctgtg caatagctct tccagggcgg attccgattg cccctgccgg tccccctcgg
      361 tcagtccgcg gcgaagatgt tcgagcaagg ccagcgactg ctcgcgcgga tagcggcccg
      421 gcgcgtcttc gaagatggct tccgcaagcc cggcagtgcc gccagtttcg gcccggaacc
      481 tgtggcgatg ccagttcacc tcggtcagct tgttgagcac atcagcggcg aattcttcaa
      541 agtcttgacc gccactggca gccagttccg cttccagcag aacgcgcctc gaaggcgcct
      601 cggtccagtt gtcacggcaa gcggccaaaa gcaacagcac gctccccagc ggcggcagac
      661 cgtgcggcgc gagccgtgcg agcactccct ccagaaattc gcggtacgcg atcgtcacgc
      721 cgtcattcca ggccagcacg cggccattct cctcccaccg ccagaacgag cccgcggcag
      781 gggcgaaata gtcgcgaatc tcctcctggt cgaggatcat cgaaggagcg cctcctgagt
      841 gccgcggagg gccgagcggc tggcgcggac ccattggtcg ggagcgatgc ggtcccatgt
      901 gccgtcaggc cggagcaagg ccacttcgtc tttcgcgagt tgcagcacct ggcggacgac
      961 ctccgcatcg accggcggcg accacgtgaa gccagccggc gtggcgacgc cttccacttc
     1021 gacccattgt gtgcccggaa tcggcgctag cggcgtgccg cggacgatcg cccggccgtc
     1081 cgccgtggcg gcaaacgtcc agcgctcgat ccgccactgc ggagcggtgg ccacatactg
     1141 cgtcaagctg gacagacttg tctccagcag cgtcggttcg cgcgcgtcgc cggtcggaac
     1201 gagcgacagg tttgtcgcct ttcgctccgc gaaagggcgt tcgttcgcgg agcgaacgac
     1261 gacattcttg cgttccacta acttcaccat cggcagcgaa acctgcagcc aatcggccag
     1321 cagcacccag ccctcgcccg gcagccggcc gagtggcacg aggcagccct cgggcacgag
     1381 ctgcccgtcg tccagcgcgg caaaccgctc cccgccggga atccgcttta gttcgcgctc
     1441 gagcgattca tcgagcgcat tgccgcgcag ccagattccc tcgccggctt cggacacggc
     1501 caatgccgga attcgccgca gacgggcaag tccggctgcc gactcgcgcg gcacgagcaa
     1561 ggcccagcgg acattcacgg ctgcactccc aattgttgcc ataattcggc cacgcgctga
     1621 tcgaggaatt cgcgctgctg ggcgttcacg acccattggc agcggcccga cagcaagccg
     1681 agctggtcgc gcaagacggc ccgctccgct tccgccagcg cggcctcggc caggcgctcg
     1741 ccgatccgtt tcaggtcctt cgccagctcc tcgggatcgg ggccgtccga ttgccgcgcc
     1801 cgcggatgac ttccctccag ttccacagcc gggctcgctt cgaccgcctg attgacgatc
     1861 gcggcgatca cctcgcgctg ctcatcgaca tcccagatgt accgcagaat ccacaggtcg
     1921 gtcgtgttcg ccgctgctcg cccgcacaag gccgcactcg ccgccaccag ccgctgcagc
     1981 ttgaccgccc gccggtcgct gacgggaatg cccgcctgcc gcagcttgtg gaccaggtcg
     2041 atatacgtcg tccgcaccgg cgcgagatcg acctcggcca ccagcgactg gacctgacgg
     2101 atgtcgtcca cggcgagcgt cggcaccgct tcggccagtc cttgatcgag cttccagcca
     2161 gccgccagca cgtcgccaag cgaatcggcg gggacgttgt cgcaccgcac gcgaaggagg
     2221 aagcggtcga acagcgccgc caaagcgtca tcctccggca ggcggttgct ggccccgacg
     2281 accatgagca gcggcaggcg gcgggtctcg cggccgcggc gaaaaacccg ctcgttgagc
     2341 accatcagca ggctgttgag gatcgcgcta ttcgcattca gcaactcgtc gagaaacacg
     2401 agggccgcct cgggcagcat tccctcggta ttggtcacca gctcgccatc gcggagcttg
     2461 cggatatcga acggaccgaa cagctcgttc ggctcagtga accgcgtgag caggtaatcg
     2521 aacacgcggc catcgatccg ccgggcgagc tgctggacca gggcgctctt cgcggttccc
     2581 ggcggcccaa gcaagaacaa gttctcgccc cccgccaggc agacgcccaa gaggtcgatg
     2641 atttcgtcct taccgacgaa cgcggctttg agcgaggcca gaacttccgc attcagccgc
     2701 cgggccaggg ccaggcggca gtcgtcaggc gggggatgca tggagtctgg agtgcggaag
     2761 gggtcgggag tcaattgcgg cattgattat ccattatgga caacggtgat cgcaattgac
     2821 tcccgacccc gttggggccg cgtcaggcca cagcccacca tgcgcaccaa gcgaccgccg
     2881 gaccgcttcg gcaaccattg gctgcgccag ccgcgaccag tcttttttcg ccagaatccg
     2941 atcgacatac aactggacga ggccgggatg gctcgtgaag tcgtcctcgt gtcgcggcga
     3001 cgtagcggcc attccgacgg acgacagagg ccaatcggcg gcccactggc gcagtttcgc
     3061 gacgagcggg tcctgctggc tggcggccga agcgtggcgc accaggtcgg gtaaatatcg
     3121 aaacacgata tcgaccgacc catgccgggc cgcccgttcg cctgcgggac atacagcacc
     3181 aagcagttct tcgatcgcct gggcgtccag atcgcgatac accgcgagtt gacaagcccg
     3241 gtagaacgaa gtcgccgccc acagcgccgc cagcggaaag agaggcggcg gctggcccgg
     3301 gaattccgcc gccagcaccg cggcctgatt ctgcaacttc acgagcgcca ctttctcatc
     3361 ggccggcgta atcgccgcct gcggccgcag cacctgcacg cggccatgat caaatagcgc
     3421 cgccagaaac gcgtccagct tcatttcttg atgcgcggga tggcgggcgg caccgggatg
     3481 gcgccggaca cgtcctccac caccggcgcg gtcccataga tggccaccgt ttcccagaag
     3541 aactcgtcgt cgctcgtccg cttgattccc agatcgaccg accagttcac tgtctgcccc
     3601 ggcaagagcg gcgctccatc cgagccgagc gtggcctgcc tttcgatctt tttgccggtc
     3661 tgcttatcgg tcaggcgaac gagaatgcgg atcgacgtga ccgtgaaagt cgaacggttg
     3721 ccgaactgcg tatcgaacca cgtcgtcccc tcgcggaagc cgcccgaata gccgtggacc
     3781 ttctcggctt ccgccggcgg gagacgcttg gcaaagccga cctgcgcgcc gccgaggcgg
     3841 tgctcgaaat gctgctcaat caacgcgcgg acgcggctgg cccggaattg agactccagg
     3901 ccctggagcc ggtccaaatg cacgtacagc ttctcctgga gcagcgcccg cacgactcct
     3961 cctagcttcg taaagtgctg atcggcatcc gcatcggtcg cattttccgg gagcacggct
     4021 gagttcaggg attcatccac caccgccagc cccaattcca ggagttccct ctcggccagt
     4081 tcacgctcgg cgaactgctc gctttctaag ccggctgcca gcctggagat gtcggcagcc
     4141 gccacccatc ctggcaacag gcaggcagcg atgatcgggg tagtatgcgc atgaaaggaa
     4201 ttccaggaga tcatactgaa atggtcctca attcacgcgg cagtggaaaa tgcacgtctt
     4261 cgctgcgaat cgtgcggatc tcgaccgtgg catggtgagt ctgacgcagg cggccgatgc
     4321 actcttcgac gatcgattcg ggtgcgctcg cgccggcggt cagcacgatc gtactggcgc
     4381 gggtcagcca gtcgtcgtcg atgtcgttcg gtccgtcgac gaggtatgcc ggcacaccca
     4441 gctcgagaga gatctccttc aaccgctggc tgttggaact gttctggctg cccacgacga
     4501 gcacggcgtc ggcttcgcgg gcgagcaacc gaatcgcttc ctgacggttt tgtgtggcat
     4561 agcagatgtc ctctttcggc gggccaacga cctggggaaa gcggcccttc agccggtcga
     4621 tgatccggtt ggcgtcatcg accgaaaggg tcgtctgcgt cagataggcg agtttcgtac
     4681 ctgaggggaa ttccaggcgg tctgcatgtt cgaccgattc gacgagcaca atcgcctgcg
     4741 gcgcctcgcc catggtgccg atgacttcgt cgtgtccttc gtggccgatg agcaggatcg
     4801 tgtagccttc cttcgcaaag cggatcgcct ccaggtggac cttggtcacc agcgggcagg
     4861 tcgcgtcgat ggcgcgcagt ttccgctcgc gcgcgatgcg gcggatttcc ggagagacgc
     4921 cgtgcgccga aaaaagcagc gtcgagcctt ccgggacctc ctccagcgaa tcgacgaacg
     4981 tcgccccctt ggcctgaaac gtctcgacga cgtacttgtt atggacgatc tcgtgataca
     5041 cataaatcgg cgtgccgaac gcgcggattg ccaggtcgag gctttcgatc gccatgttca
     5101 cgcccgcgca aaacccacga ggcgcagcca aaaggatttt catgcagtgg ggctcccctt
     5161 gaatgataac ttgcttccca gcaatgggtg agtggatctc gcgcttcctt aaccgcagcg
     5221 acggcacgtg ggaaacagga tgcagtgata actgtggcgc aatcgacgcg cggatgaccg
     5281 ccattggctg cgtcacttcg ctgtctctgc gaccgtgacg ccattctgtt caaccagctc
     5341 ccgcacgccg gccagcagag cgtctgcttc agcgggctga cgcagctcga tcacgtggag
     5401 atgagcaaat gcgggccctg gcagcaggct tcgcagctcg cgctgccgcg aacggaacgt
     5461 tcggagcacc caccagggaa tgccgtccca atggaaaaat gacttggcga cggtctcgcg
     5521 gttgctgccg cagatcgcct cgccggttcc gatccgctgg caggttcggg tgagcgatcg
     5581 ccagaagacg agcggcaggc cgagattgag ccagacgagt gccgttgccc gcgcccacgt
     5641 caattcgcga accatcgtat agttgccatc gacgatccac tcctcggctg ccgtcgcttc
     5701 gtccacgcgg ctgacgaaat cgggctgcgg cgtccaattc ggcccccaat agagcgcgtc
     5761 cagctcgatg tgcctccgcc ccagccgctg ggccaactgc cggctgaaag tcgtcttgcc
     5821 gcaacagctt gtgccggcaa cgacgatgcg ggggtgatcg gccagcgacg agagctcact
     5881 catcccacgg gttcttcttt ttccgcggcg aaagctcggg gtgctgggcc aattcgtctt
     5941 ctatgactcg ctgcggtgcg gcggcgcgga cgtgttccac ctcacgggcg gcaacgacga
     6001 ggttaacgag gaaaatgacc agccccgcgg aggcgaccgt ggcggtcagc tcccagccgc
     6061 tgaagaccgt ctgctcatga acttcgtaca gcgtccagac ccagttggga agctgcacga
     6121 gcgaatagga cgagttggaa aaatcgcccc acacgctgac cacgtgcatc aggtacggaa
     6181 agagcaccgc cccgctggcg gcgatcacct ggcacaggaa cgtggccagc aggccgacgt
     6241 gtgtgacccg ccgcagggcc acgatcatca gccgcaccag gcccaaatat cccagcacat
     6301 agcccacgag cgtggcgctg aagtagtaat actccggcaa ggccggatag tactcgaagc
     6361 cgccgatcaa ggcggcgcta acgccgatcc acgcaatggc aagcacggaa agcaggttga
     6421 gcattgcgaa gacatagccg gtgcccgagc cgggattgaa ccaggtgaag gccatccgcc
     6481 cgaggaagct ttgcgggagc tgccgcttgg cccgtggcga aagctgggcc gtttcgccgg
     6541 tgagaaaggc gccgatcacg gcccagaacc cggccgcgac gatgatctgc ccgaacagca
     6601 gctcacccgt atcggtccgc agccaatagt aagtgaacca ggccacgatc agcacgtgca
     6661 gcccagccaa caagactcgc aagggccccg agcggttctc gctcgcgaac gtgatctgcc
     6721 cggcgccgat ctgcaagagc agggcggcga ttggcaggta gaagctcagt ccgcagaggt
     6781 tggcgatcca gaaatcggcc tcgtcgaagg ggggcgtctg gcggttgacg atgaccgcga
     6841 tgatgaagaa gtcccaaatg accgtgaaga ccagcagggc catcacgaac acaaccgaga
     6901 gcagcacttg ccaatgcctg gcccgcgtga cggtggccag catcaggccg acgatgctca
     6961 atagcagcga cgcgaggaac gtgtacgtga ggaacatgcc gatcgtcagc acgtcgatcc
     7021 cgcgcaacag gtacgtgaac gcgatgcagg gggccagcgc cgagtaatag accatcatct
     7081 gcagcaccgc gctgccgagt ttgcccagca cgatctgccg ggcgctaagc gacgtgatgg
     7141 agagcagctc gtacgtgccg tcctcgcatt ctcctgccag cgagcggaac gccgcgtagg
     7201 gcacgacgat caggagcggg atcgacagga cgatgtaata accggtgagc accgccggcc
     7261 cgaccggcgc gaagaacacg tccggcacgc tgagggcgat gaagatcacc gtccacagcc
     7321 agccgaagat gaggagcagc gagaaggtca ccacgaactg gcggctcttc atcgcctggc
     7381 gggcttcctt gaccagaatg ggattcagcc aatcgccgac ccggtccagg accgtctcga
     7441 atcgcagcca ggtctgctcc agcccggcgg tttttgcggt tttatccgcg tgaacttccg
     7501 ccgccagtgc cgccgccgcg ggcgcagccc cgccgccagc cggcgcgtcg gctgcctggt
     7561 gcgggctcgg cgattgcgtc gtggaatcca tcggtcaggc cagacctcgc ggcaatggcg
     7621 aagcaaagag aaatcgatct taccacagcc ccgcgcccga aacggggcca agcattggga
     7681 gcgcgcccgc tggcagtccg cggccagccc ctgccgaatg ggggaggcga cttgccgggc
     7741 ccttgcgacc aggcgcgcga ggacgattac tccgaaggtt aggattagat tcttagggcg
     7801 tgagatgcgc gtggcgggcg cgcatgtgtg gagttgagtt taggcggcgt tggcgagtcc
     7861 gtggtagtag agctggaggc aggcgccgag ggtgcggtgg ccgcgggggg tgactttcca
     7921 gcgg
//
LOCUS       DASUGB010000603         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_622497, whole genome shotgun sequence.
ACCESSION   DASUGB010000603 DASUGB010000000
VERSION     DASUGB010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622497"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VFV87_11570"
     CDS             complement(<1..1110)
                     /locus_tag="VFV87_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5104445.1"
                     /translation="MKRARRRIDVNLEELDRVLDGAREAPLNEADYDKLKDALHAMAA
                     LLARSPNTEKTSVVVGDAAGGETGDGDGPDVGGPPPAGHGRNGARAFAGAERVAIPHQ
                     SLKHGDRCPECGQGNVYVQKEPKVLVRIVGQAPLAATVYSLERLRCGASGQVFTAEEP
                     EGVGSDKYDETAAAMIAQLKYGSGIPFYRLERLEGQLGIPLPAATQWEIVEELAEVIK
                     PARDELIRQAAQGEVLHNDDTGMRVLKLEREPGDQRTGVFTSGIVSTAQGRKIALYFT
                     GRQHAGENLADVLKQRAVVAGPAIQMSDALSWNAPKLPGGVELLVAHCLAHGRRQIVE
                     VAQNFPVECRYVLEQLGEVYGVDAQARDAGMTAEER"
     gene            complement(1107..1982)
                     /locus_tag="VFV87_11575"
     CDS             complement(1107..1982)
                     /locus_tag="VFV87_11575"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HEX5104446.1"
                     /translation="MATLRYRGREIHPEDILHIRALIEQHAGDSRRRLSTRLCEAWDW
                     RQSNGALRDMVCRGLLLMLERAGQIALPPVTYKRHNPLAQRARPAPVLLDQTPLEGRL
                     KDLGPIELEKARRTADEPLFNSLMEEHHYLGYEQPVGEHLKYLVWAQGRPIACLAWSS
                     APRHLGSRDRYIGWSAEARRRNIRFLAYNTRFLILPWVRVEHLASHILGRMAAEISDD
                     WQRMYGHPIYFLETFVDPDRFRGTCYRAANWVLMGKTTGRGKQSNSYVPNRSIKEVLG
                     YPLTRRFRELLEQSE"
     gene            complement(2029..2388)
                     /gene="tnpB"
                     /locus_tag="VFV87_11580"
     CDS             complement(2029..2388)
                     /gene="tnpB"
                     /locus_tag="VFV87_11580"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX5104447.1"
                     /translation="MIQITAQMRVLVAIEPVDGRKGIDSLARVCREKLAEDPFSGCVF
                     VFRSRSGTAIRLLTYDGQGYWLAQKRLSKGKFSWWPEAVDPAKPLEAYEAQLLLAAGD
                     LSRVRAAPMWRRVTALK"
     gene            complement(2385..2762)
                     /locus_tag="VFV87_11585"
     CDS             complement(2385..2762)
                     /locus_tag="VFV87_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104448.1"
                     /translation="MAEKADGMETARRRFEQWRSSRSRKSPIPDELWKLAMDAARQQG
                     VNRAAQQLRLDAGKLKRFLVAADSRSRKAQFVELIAPAVAASPECMIEFESAAGSKMR
                     VHWKTAAAPDWANLLRAWRDSER"
BASE COUNT          470 a         1101 c          850 g          555 t
ORIGIN      
        1 ccgctcctcc gccgtcatcc cagcgtcgcg cgcctgagcg tcgacgccgt acacctcgcc
       61 cagttgttcc aatacatagc ggcattccac cggaaaattc tgcgccactt ctacgatctg
      121 acgccttccg tgagcgagac agtgagccac cagcagttcc actccgcccg gcagcttcgg
      181 cgcattccac gacagcgcgt cgctcatctg gatggccggc cccgccacca ccgcccgctg
      241 cttcagcacg tccgccagat tctctccggc gtgctgccgc cccgtaaagt acaacgcaat
      301 cttcctgccc tgcgccgtgg aaacgatgcc gctggtgaac acacccgtgc gctggtcgcc
      361 gggctcgcgc tccagcttca gcacccgcat gccggtgtcg tcgttgtgca gcacttctcc
      421 ctgcgccgcc tgccggatca gctcgtcccg cgccggcttg atcacctccg ccagctcttc
      481 cacaatctcc cattgcgtcg ctgccggcag cggtatccca agctgacctt ccaatcgctc
      541 cagccggtag aacgggatcc cgctgccgta cttgagctgc gcgatcatcg ccgccgcggt
      601 ctcatcgtac ttgtccgacc ccacgccttc cggctcctcg gccgtgaaca cctgcccgga
      661 cgctccgcac cgcaaccgtt ccagcgaata caccgtcgcc gccagcggcg cctgccccac
      721 aatccgcacc aggaccttcg gctccttctg cacgtacaca ttgccttgcc cgcattccgg
      781 gcaacggtcg ccgtgtttca gactctggtg cgggatcgca accctctctg cgccggcgaa
      841 cgcccgggcg ccgttccgtc catgcccggc cggaggaggc ccgccgacgt ccggcccgtc
      901 gccatcgccg gtctcgccgc ccgccgcatc gcccaccacc acgctggtct tctccgtatt
      961 cggcgaccgc gccaacagcg ccgccatggc gtgcagcgcg tccttcagct tgtcgtaatc
     1021 ggcttcgttc aacggcgctt cgcgggcgcc gtccagcacc cggtccagtt cctccaggtt
     1081 cacgtcgatg cgtcgtctgg cccgcttcat tccgattgct ccagcagctc gcgaaaacgt
     1141 ctggtcagcg gatagcccag aacctctttg atcgaccggt tcggcacata gctgttggac
     1201 tgcttgccgc gtccggtggt cttgcccatc aacacccagt tcgccgcccg gtaacacgtc
     1261 ccgcggaatc ggtccgggtc cacaaacgtc tccagaaagt agatcggatg cccgtacatc
     1321 cgttgccagt cgtcgctgat ctcggccgcc atgcggccga gaatgtgcga agccagatgc
     1381 tccaccctga cccagggcag aatcagaaag cgcgtgttgt aggcgagaaa gcggatgttc
     1441 cggcggcgcg cctccgcgct ccagccgatg taacggtcgc ggctgcccag gtggcgcggc
     1501 gccgacgacc aagccaggca ggcgatcggc cgcccttgcg cccacaccag atacttcaga
     1561 tgttcgccca caggctgctc gtagccgagg taatgatgct cctccatcag gctgttgaac
     1621 agcggctcgt cagcggtgcg cctcgccttc tcgagttcga tcggcccgag gtccttcagc
     1681 cggccttcga gtggcgtctg atccagcagc accggcgccg gccgcgctcg ttgcgccagt
     1741 ggattgtggc gcttgtaagt gaccggcggc aaggcgatct ggccggcgcg ctccagcatc
     1801 aggaggagac cgcggcacac catgtcgcgc agggcgccat tgctttgccg ccagtcccag
     1861 gcctcacaca accgcgtcga aagcctgcgg cggctgtctc ccgcgtgctg ttcaatcagc
     1921 gcccggatgt gcaggatatc ttccgggtga atctcccggc cgcgatatcg cagcgtcgcc
     1981 attgcccccg tagtatgaac ctgttttctt tggaacgcaa ggggaaaatc acttcagcgc
     2041 ggtcacgcga cgccacatcg gcgcggcccg cacccgcgag aggtctcccg cggccagcag
     2101 caactgcgcc tcgtaagcct ctagcggctt ggccggatcg actgcctccg gccaccagga
     2161 aaatttccct ttcgagagcc gcttctgcgc cagccagtag ccttggccgt catacgtgag
     2221 gagtcggatt gccgtcccgc tgcggctgcg aaatacgaac acgcatccgg agaagggatc
     2281 ttcggcgagt ttctcccggc acacccgcgc cagcgagtcg atgcccttcc ttccgtccac
     2341 gggctcgatg gccaccagca cgcgcatctg cgcggtgatc tggatcatcg ctcggagtcc
     2401 cgccaggcgc gcagcagatt cgcccagtcc ggcgccgcgg cggtcttcca atggacgcgc
     2461 atcttacttc cggcggccga ttcgaactcg atcatgcatt cgggactggc ggccaccgcc
     2521 ggcgcgatca gttcgacgaa ttgcgctttc cgactccgag agtcggccgc tacgagaaac
     2581 cgcttcagtt tgccggcgtc cagccgcagt tgctgggcgg cccgattgac tccttgctgc
     2641 cgggccgcat ccattgcgag cttccacaac tcgtccggaa tcggcgactt ccgcgagcgg
     2701 ctgctcctcc attgctcgaa ccgcctccgc gccgtttcca ttccgtcggc cttctctgcc
     2761 atcgctctcc tcgctcaagg atgaccgtag cagtcgcgac cgggtccatt cacgcatcct
     2821 tgccgaaagg acacacacca tccgggagtt ggaggagcac agcatccgtt tcgtggccgt
     2881 gacgcaggcg ctcgataccg atacgaagaa tccagcgtcc cgcttcttgc tgcacgtgct
     2941 gggcgcggcg gcggagtttg agcgggccct gatccg
//
LOCUS       DASUGB010000604         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_485457, whole genome shotgun sequence.
ACCESSION   DASUGB010000604 DASUGB010000000
VERSION     DASUGB010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485457"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFV87_11590"
     CDS             complement(<1..477)
                     /locus_tag="VFV87_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5104449.1"
                     /translation="MLLIATAASQTPAQEPSALETAAAMERLVTDAIEKAEKSVVAIA
                     RVRREKDAPAREPEDLALPPFPLDRDVGPTDPKFVPNEFGTGVVIDAKGLIVTNYHVI
                     GDVRGADYYVWLARKPYAAKVKAADPWLDLAVLQIEAENLQPITFGDGRAVKKGQFV"
     gene            complement(1044..2183)
                     /gene="recA"
                     /locus_tag="VFV87_11595"
     CDS             complement(1044..2183)
                     /gene="recA"
                     /locus_tag="VFV87_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5104450.1"
                     /translation="MAATLVHEEKSNHRMATATKKEKPPRENGRPAKSAALSAIEDNG
                     VLKSTLQQIEKQFGEGSIMPLGGDKTLTISGIPTGSLSLDVALGGQGIPRGRIIEIFG
                     PESSGKTTLALHVAAQAQKAGGIAAIIDAEHAFDPTWGKKLGVQLETLLVSQPNNGEE
                     AMQITEMLVKSNSVDVIIIDSVAALVPRAELEGEIGDTHVGLQARLMSQSMRKLTGAI
                     AKSKTAVIFINQIREKIGVSYGSPETTPGGRALKFYSSCRIDVRRIGQLKDGEEVVGQ
                     RVRTKVVKNKVAPPFRVAEFDMMHTNGISYEGDILDLGLAQKILTRSGAWFKYGETYL
                     GQGKEKARAFLMENPQVTQELREKILSSGALDGGVASEPAGAEEE"
     gene            complement(2398..>3048)
                     /locus_tag="VFV87_11600"
     CDS             complement(2398..>3048)
                     /locus_tag="VFV87_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5104451.1"
                     /translation="AWTDCGQVGPEENTQTYAFAAYRGELYVATWRTGRVYKYRGDND
                     WLDCGRLGEELEVMGMAVHNGQLYAGTLPSAEVYRYDGETTWTKVGTLDTTPDVKYRR
                     VWTMAEFNGRLYCGTLPSGKVWSMSAGYNVTCDKELPTGWHHVAAVRCGQHLRLYLDG
                     KQVAAGQWQGLPLVNIENDAPLLIGFGPTDYFHGALRDVRLYSRALADVELAALAK"
BASE COUNT          516 a          960 c          980 g          592 t
ORIGIN      
        1 gacgaactgc cccttcttca ccgcgcggcc gtcgccgaac gtgatcggct ggaggttttc
       61 cgcttcgatc tggagaacgg ccaggtcgag ccacggatcg gcggctttga ctttggcggc
      121 gtagggcttg cgggcgagcc agacgtaata gtccgcgccg cgcacgtcgc cgatgacgtg
      181 atagttggtg acgatcagcc ccttggcatc gatcaccact cccgtgccga attcgttcgg
      241 cacgaacttc ggatcggtcg ggccgacgtc gcggtccagc ggaaacggtg gcagcgcgag
      301 gtcttccggc tcgcgagccg gcgcgtcctt ttcccgccgc acgcgagcaa tcgccactac
      361 cgatttctcg gctttttcaa tcgcgtccgt gaccagccgc tccatcgcgg ctgctgtttc
      421 gagagcggat ggttcttgag cgggggtttg gctggcggca gtcgcgatca gcagcaccgc
      481 ggccgctgtg gcgaggcagc gtcgctggct ttttaacgca aaggcgcgaa gagcgcgaag
      541 gacgcaaaga ggcagggaga gaaggggaac gaggtgagtg aatgaaaaca ccccgccgaa
      601 tgaaggcccg gagggccggc aagaagtagc ccggaccgtg aggtccgggt tagcggatga
      661 taacgaggcg ggaggtccga aggaccggta catgatcggg ccggcggtcg ggcaagtgtc
      721 ggccctccgg gcctcacgag cgcggtttgg gccgtgtacc ccgacctcac ggtcggggct
      781 actaactgtc ggccctccgg gcctgaggca ggtcgccgtc atcgctatcc gtcctcagag
      841 tttcgctgtc gaatggctcg aatctcacgc caagcaagcc agacggtgag ccgcctcaat
      901 aggaatacga cgactcccat ggtagcgatc caaatcgctg ccgaggtcca gcccacaacc
      961 agcgcgatac ctgccaaacc aaagcaggcg agtgtgaaaa tccagaacga caattcgtgg
     1021 gtgcgaattc ccattgtccg tgtctactcc tcctccgctc cagccggttc gctggcgacg
     1081 ccgccgtcga gggcgccgct gctgaggatc ttctcgcgca gctcttgcgt cacttgcgga
     1141 ttttccatca ggaacgcgcg ggccttttct ttgccttggc cgaggtaggt ctcgccgtac
     1201 ttgaaccagg cgccgctgcg ggtgaggatt ttttgggcga ggccgaggtc gaggatgtcg
     1261 ccttcgtagc tgatgccgtt ggtgtgcatc atgtcgaact cggcaacgcg gaacggcggg
     1321 gcgaccttgt tcttgaccac cttcgtccgc acccgctggc cgacgacttc ctcgccgtct
     1381 ttgagctggc cgatccgccg cacgtcgatg cggcaggacg aatagaactt gagggcccgg
     1441 ccgccggggg tcgtttccgg gctgccgtag ctgacgccga tcttctcgcg aatctggttg
     1501 atgaagatca cggcggtctt gctcttggcg atcgccccgg tcagcttccg catcgactgg
     1561 ctcatcaggc gggcctgcag gccgacgtgc gtatcgccga tctcgccttc cagttcggcc
     1621 cgcggcacca gggccgcgac cgagtcgatg atgatcacat cgaccgagtt gctcttcacc
     1681 agcatctcgg tgatctgcat tgcctcttcg ccgttgttcg gctggctgac gagcagcgtc
     1741 tcgagctgca cgccgagctt tttgccccag gtcgggtcga aggcgtgctc ggcgtcgata
     1801 atcgccgcga tgccgccggc tttttgggcc tgagcggcga cgtgcagggc cagcgtcgtt
     1861 ttgccgctcg attcggggcc aaaaatctcg atgatccggc cgcggggaat gccctggccg
     1921 cccagagcca cgtcgagcga caggctgccg gtcggaatgc ccgagatcgt cagcgtcttg
     1981 tcgccgccga ggggcatgat cgagccttcg ccgaactgct tctcgatctg ctgcagggtc
     2041 gatttgagga cgccgttgtc ttcgatcgcc gacagcgccg cggacttggc cggccggccg
     2101 ttctcgcggg gcggtttttc cttcttggtt gcagttgcca tacggtggtt cgacttttct
     2161 tcgtgaacga gggtagctgc catcacgcga actcctttat ctgaaatgga tgaacaaaaa
     2221 tcaagcagta gcccgtgggc gggcgccagc gcctgcccat ccctaaccgc acgccggtca
     2281 gcggctgctc gaactaagtg gattttcgcc gcccgatgga cacgtctggt ccatttgagt
     2341 caagcgaaaa aaagaatttc gcgatttttc ttccgtccac cacgcgagga ccgtcgatca
     2401 cttggccaag gcggctaact cgacatcggc cagcgcccga ctgtaaagcc gcacgtcgcg
     2461 gagggcgccg tggaaataat cggtggggcc gaagccgatc aggagcgggg catcgttctc
     2521 gatattgacg agcggcaggc cctgccactg gcccgcggcg acctgcttgc cgtccagata
     2581 caggcgcagg tgctggccgc agcggacggc ggcgacatga tgccagccgg tcgggagctc
     2641 cttgtcgcac gtgacgttgt aacccgccga catcgaccag accttgcccg acggcagcgt
     2701 gccgcaatac agccggccgt tgaactcggc cattgtccaa acgcggcggt atttcacgtc
     2761 gggggtcgta tcgagcgtgc cgaccttcgt ccacgtcgtt tcgccgtcat agcgataaac
     2821 ctcggctgac gggagcgtgc cggcgtagag ctggccgttg tggacggcca tgcccatcac
     2881 ctccaactcc tcgcccagcc ggccgcagtc gagccagtcg ttgtcgccgc ggtatttata
     2941 gacgcggcca gtccgccagg tcgccacgta cagctcgccc cgataagccg caaaggcgta
     3001 ggtctgcgta ttttcctccg ggccaacctg gccgcagtcg gtccaggc
//
LOCUS       DASUGB010000605         9410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2013529, whole genome shotgun sequence.
ACCESSION   DASUGB010000605 DASUGB010000000
VERSION     DASUGB010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013529"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1417
                     /locus_tag="VFV87_11605"
     CDS             290..1417
                     /locus_tag="VFV87_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX5104452.1"
                     /translation="MAPLVLDVTAAEDPSDAVHRAVQALVEGKVVALPTETVYIAAAS
                     ALSESAVQRLLNLRSGQLEGPATLAVRSTEEVLDYVPQLPPIGQRLARRCWPGPVTMQ
                     LADAHPDSAIHRLPKQSRAAIVPGGQIQVRVPAHELVLRILRLLKGPVVMVGARYAND
                     PDAVTAADVVSRLGEHVDVVLDDGRCKFAQRSSIVRVNDHGFEVLRPGVLSEANLKRL
                     ASLMIVLVCTGNTCRSPMAEVLLKKQIAERLGCRMEELDDRGVVVMSAGTSAAPGGRA
                     APEAIQAMQGRGLDLTQHESQPISSRVVRFADLILTMTKSHRDAIVGQWPDAEPRVFT
                     LSPGNDISDPVGGPLELYRRCADQLDASLADWVDKLPLGES"
     gene            complement(1492..2955)
                     /locus_tag="VFV87_11610"
     CDS             complement(1492..2955)
                     /locus_tag="VFV87_11610"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEX5104453.1"
                     /translation="MNDQWYYEQHGVPHGPLAGEQLQQLVAKGAVPPTTLVWREGLTE
                     WTTAAAAGFQPVATSPQPPPLPVPPPIIAPAARANRRAPPPTINWPLVIGGGAAALVV
                     AALGGWFANALLRERDPPRQIAAAAPSVPDTVNPAELRRPAETPRPSPRPAPAALPSA
                     KSPSPSVPASSPTAEPSVPAKPAPAQIEPPPVVAKTPTDPATQSTPPAPSTPPPQTQP
                     SSNAQTLYQEIDIQRAPKFSILGSVTAQELHYKLLSELHAAAADEQGVRKIEQYVRST
                     QLLKADELSQGMFEQSLRDLEGWQFTFKVNRRGDILEWKGGPPAGRKTARVEQPGAAG
                     FMVTSVLDEDGWKEMAQLSFFVPEEQTAGSQTWVRKMTHDFGPLGSWVGETHYTPKEA
                     SNPLQIDYVHRMTYKPPARGIGGLPFAITDAKFTPEIAAGAFLYDKQASRVIQVQERF
                     LVKGSLATELLGQAAAVEMEEDQLITVRLLEQNPWEQ"
     gene            3110..5344
                     /locus_tag="VFV87_11615"
     CDS             3110..5344
                     /locus_tag="VFV87_11615"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX5104454.1"
                     /translation="MRLLMGFIFLAMPVLAAAEEPAPVTTVWNVDRLDRLGEYPTTVL
                     GAPSLVETKDGKAVQFDGQDDALLVDANPLAGLKQFTVEVIFQPQAAGPKEQRFLHFQ
                     EAGSENRLLFETRLTDDGQWFLDTYVKSGEHDATLFAEKSLHPIGPWYHAAVVVDGQT
                     MRHFVGGQEELKADLPFAPLGPGQTSLGVRINKVFWYKGAVRQIKITPRALAPQEFVS
                     LAPAAPAIVARAVKPLANPNLDEQLDLVYAKYGERELHLDLFRPKDQGDRALPAVLVV
                     HGGGWLQGDKTRFRALGQALALRGYVAAVIEYRLGGEARFPAAVHDCNAAIRWLRANA
                     KQYGIDPERVAAVGGSAGGHLVGLVAAAPDVRELQGDGGNAGQSSRLQAAVVLAGPME
                     LATGPVAERSRQNPEISNTNRWLGKTIDEAPELYRLASPFTHWSKAAPPILFMHGELD
                     HPEQNAASRRRLQELGVPAGVLVYTAGKHGCWNQHPWFEPMVDDIDAFLTSALQRDLY
                     LQWLPKIETDWGEMRFGAESVDLLIFHKPAGGVLQIPRLNNPVKVAYVRGDAAKTPLK
                     LSPGVREWMIDVSPLPGGAIGQTVIVETVGRPHVPTIPRVVGGSADGSIVLHAHDAVT
                     HGENLRYEPQPHKNTVGYWTNEDDWCEWRCYVEQPGKYELQILQGCGKGQGGSEVEVQ
                     IGDQFVTFTVEDTGHFQNFKERSLGTVTLAAPQVNSLQIRAKTKAAAAVMDVRQVRLI
                     PVAD"
     gene            complement(5357..6778)
                     /locus_tag="VFV87_11620"
     CDS             complement(5357..6778)
                     /locus_tag="VFV87_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104455.1"
                     /translation="MAIPVPNSPPLDAIRRLSRRKLLAASVGLGALSLPAFLQTRAAA
                     AETSDGTVGTALRGVPSGKAKSCIIIYTWGGMSHLESWDLKPDAPVEIRGEFSPIATA
                     TPGIQISEHLPLLARQTEKLAIVRSIFHDDSAHGRGMYWNLTGHAPPRAGNIPPFPND
                     WPSLPAMVSKLRRAPKGVPPAVRIPYPMVDNDTLQAGEYGGWLGVKYDPIVMRPPAGE
                     PFGGVSQTLGSEVLELKNSDFDRVAARKELLATLDKPQPRRDEFDGFNHFRQLATDIL
                     LGSAVRNAYDLDREDPQIRESYGSHLGGQSMLLARRLIEAGVPVVQVCCAAGDLNGGA
                     GDMWDTHSDNFNRLKNRLLPVFDRGVSALLQDLADRGTLDETLVVMLTDFGRTPRING
                     GAGRDHYPSVYSVALAGGGIRGGQVYGSSDSNGAFPREKPCGPPDIHATIFQQLGISP
                     RAEIHDQLGRPFAVSDGHPLPLT"
     gene            7088..7696
                     /locus_tag="VFV87_11625"
     CDS             7088..7696
                     /locus_tag="VFV87_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104456.1"
                     /translation="MLRQLGIAFGVSLLLLNGEMLTAQESCSLTIPVKAVGAGTTFFQ
                     TFQFREGTPSLPGLLSGNVEIGGQVVQEVKIRIARQVQREVVEYVVVYVDGRPTVQRR
                     VSLVVETVYVYVPVLQEIVLPVFTGTWTSVDLCFYSTWNILHVGTDGMVVGVGTTVGN
                     QLYGSALYFGVAGIPNGLYSLSTGVPGDGGGGGGGGGPPVEP"
     gene            complement(7693..>9410)
                     /locus_tag="VFV87_11630"
     CDS             complement(7693..>9410)
                     /locus_tag="VFV87_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104457.1"
                     /translation="LARGRGLQSFDAAPTHRGGRLQLDRLALGEALADAEAKDGITAS
                     EFGYTANAISPQTGKLVESARALFEKATGKEAPADRSEVQRLVAKACLYDTAAANVGQ
                     SDGGIAFQAIEKLEELHAADVKDWSAKLPQLKTKLDLVIRDQSLAEALDQVATAAKID
                     INLLEGSVADATALIGPDAARVSYLDLRHATTSQALDWILQPARLTWRVDGKAVVAGS
                     ERRRAGESGWTYDVGAIALPLEKDLTDLNDYNKAVEAAKKAADEFLAAVRKELKADDE
                     TSVTWFAPGQLLVIGQPDRHAAVTKLIATLQEGKTKPTGALSAISEVTRTRYAARKES
                     VAKAAAAEALLNVAAAHDQFSWQLLSAAAGGELDLEALTELQIAWKSPQTAELLGGAG
                     RPLAMRSLWTIGQAAKALPGETELAALAAEARKQLPAAAEAALAAAEKDKNDQAALAS
                     VLYAVLANPSDETLRGKAAELFAVQSGEDSAADLRLLGRMLVGNPAATDAESLRELVA
                     KHVAGPDAVVLTALACRQAGGEAWDQFRAESRDLLGGQPLPGEVVALINRLDQSSTAL
                     ARTGD"
BASE COUNT         1534 a         3077 c         3136 g         1663 t
ORIGIN      
        1 gatccccagt cctacggcca gcccatcgac gagaagcact tcatggaggt ctggatcgcc
       61 ctgcagaacc tcaaggactt cttcgccgca gccgcggaga atatggaggc ggtagtgttt
      121 accgccaagc tgacggcata gcccggatag cacttgactc cagacgtaag tgcgtgaaaa
      181 ttaacgaatt agggcgaatt ctttgccgac cgctctgacc ccgctgggcg accccgcaca
      241 ggcgggcgcg ggacgagcgt ggatcactgc cgcttgagct ggcgatttga tggctccgct
      301 cgttctcgat gttaccgctg ccgaagaccc cagcgatgca gtccaccggg ccgtgcaggc
      361 gctggtggag ggcaaggtcg tcgcgctgcc gaccgagacg gtctatatcg ccgcggccag
      421 cgccctcagc gaaagcgcgg tccagcgcct gctgaattta cggtccggcc agctcgaagg
      481 gccggcgacg ctggccgtcc gcagcaccga ggaagtgctc gactatgtgc cgcagctccc
      541 gccgatcggc cagcggctgg cccgccgctg ctggccgggc ccggtgacga tgcaacttgc
      601 cgacgcccat ccggatagcg cgatccaccg ccttccgaag cagtcgcgcg ccgcgattgt
      661 gccgggtggt caaattcagg tccgcgtgcc cgcgcacgaa ttggtgctcc gcatcctgcg
      721 gctgctgaaa ggcccggttg tgatggtcgg cgcacgttac gcgaatgatc ccgacgcggt
      781 gaccgccgcg gacgtcgtct cgcgactggg cgagcacgtc gatgtcgtcc tggacgacgg
      841 ccgctgcaag ttcgcccagc gctcgtcgat cgtccgcgtc aacgaccacg ggtttgaagt
      901 gctgcggccc ggcgttcttt cggaagcgaa tctcaagcga ctcgccagcc tcatgatcgt
      961 cctggtctgc accggcaaca cctgccgcag cccgatggca gaagtgctcc tcaagaagca
     1021 aatcgcggaa aggctcggct gcagaatgga agagcttgac gatcgcggcg tggtcgtcat
     1081 gtcggccggt accagcgccg cccccggcgg gcgagccgcg ccggaagcga tccaggcgat
     1141 gcagggccgc ggactcgatt tgacgcagca cgagagccag ccgatctcct cccgcgtcgt
     1201 gcgatttgcc gacctgatcc tcacgatgac caagagccac cgcgatgcga tcgtcggcca
     1261 gtggcccgat gccgagccgc gggttttcac cctctcgccc ggaaacgaca tctccgaccc
     1321 ggtcggcggc ccgctggagc tgtaccgtcg ctgtgccgat cagctcgacg ccagcctggc
     1381 ggattgggtc gacaagctgc cgctgggaga atcgtagccc gcagcgcaag cgagggtaat
     1441 cggcttgcca accggactac cctcgcttgc gctacgggct aagatgcgcc gtcactgctc
     1501 ccaaggattc tgctcgagca gccgcacggt gatgagctga tcctcttcca tttcgacggc
     1561 ggcggcctgg ccgagcaatt cggtggcgag gctgcctttg acgaggaacc gctcctgcac
     1621 ctgaatgacg cggctcgctt gcttgtcgta aaggaaggcg cccgcggcga tttcgggtgt
     1681 gaacttcgca tccgtgatgg cgaagggcag gccgccgatg ccgcgggccg gcggtttgta
     1741 tgtcatccgg tgcacgtagt cgatttgcag tgggttgctt gcttccttgg gcgtatagtg
     1801 cgtttcgccg acccagcttc ccagcgggcc gaagtcgtgc gtcatcttgc ggacccacgt
     1861 ctggctgccg gctgtttgct cctccgggac gaaaaacgaa agctgggcca tctccttcca
     1921 gccgtcctcg tccaggaccg acgtgaccat gaaaccagcc gcccccggct gctcgacgcg
     1981 ggcggtcttg cgtcccgccg gcggtccgcc cttccactcg aggatgtcgc cgcggcggtt
     2041 gactttgaag gtgaactgcc agccctccag gtcgcgcagc gactgctcga acatgccttg
     2101 cgagagctcg tcggctttga gcagttgcgt gctgcgcacg tactgttcga tcttgcgaac
     2161 gccctgctcg tcggccgcgg cggcgtgcag ctccgagagc aacttgtagt gcaactcctg
     2221 ggcggtgacc gaaccgagga tcgagaactt gggcgctcgc tggatgtcga tctcttggta
     2281 gagcgtttgg gcgttggacg atggctgcgt ctgcggcgga ggcgtgcttg gagccggtgg
     2341 cgtcgactgt gtggccggat cggttggagt tttggcgacg acaggcggcg gttcgatttg
     2401 tgcgggcgcg ggctttgccg gtaccgacgg ctcggcggtc ggagacgaag ctggaacgct
     2461 cggcgagggg gactttgcgg aagggagagc tgcgggagcc ggccgcggac tcggacgcgg
     2521 cgtttcagca ggtcggcgca gctcggccgg attcaccgtg tccggcacgg agggcgccgc
     2581 ggctgcgatc tgtcgcggcg gatcgcgctc gcgcagcagg gcattcgcga accagccgcc
     2641 gagagcggcg acgacgagtg cggccgcgcc gccgccgatg accagcggcc agttgatagt
     2701 cggcggcggc gctcggcggt tggctcgcgc tgctggcgcg atgattggtg gcggcaccgg
     2761 caaggggggc ggctgcgggc tggtcgcgac tggctgaaat cccgcagcgg cggcggtggt
     2821 ccattcggtc agaccttcac gccagacgag cgtggtcggt gggaccgcgc ccttagccac
     2881 gagctgctga agctgctcgc ccgccagcgg cccgtgcgga acgccgtgct gctcgtagta
     2941 ccactggtcg ttcacagcgg gctccgccga gatgcctcaa accttgcctg cacaccgcct
     3001 ctttgaattg tcattcattc ccgcggtcct ggcaacgaaa actcgcaatt caggaggcct
     3061 gcggatagat tcggaagtac aaatcgccaa tctcgactaa ggatacgcta tgcgcctgct
     3121 catggggttc atcttccttg cgatgcccgt tctcgccgct gcggaggaac cagcgccggt
     3181 caccacggtg tggaatgtgg accggctcga ccggcttgga gagtacccga cgaccgttct
     3241 cggcgctcca agcctcgtcg aaacgaagga cggcaaggcg gtccagttcg acggccagga
     3301 cgacgccctg ctcgtcgatg ccaatccgct tgccggcctc aagcagttca cggtcgaagt
     3361 catctttcag ccgcaggccg ctggtccgaa ggagcagcgg tttctgcatt tccaggaagc
     3421 gggcagcgag aatcgcctgc tgtttgaaac acgcctgacg gacgacgggc agtggtttct
     3481 cgatacgtat gtgaaatcgg gagagcacga cgcaacgctc ttcgccgaga agtcgctgca
     3541 cccaatcggc ccctggtatc acgccgcggt cgtcgtcgat ggccagacga tgcggcattt
     3601 cgtgggaggg caagaagagc tgaaagccga cctgccattt gcccccctgg gccccggcca
     3661 aacctcgctt ggcgtgcgga tcaacaaggt cttctggtac aagggagccg tgcggcagat
     3721 caagatcacg ccgcgggcgc tcgcgccgca ggaattcgtc tcgctcgctc cggcggcgcc
     3781 agcgatcgtc gcccgcgccg tcaagccgct ggccaatccg aatctcgatg agcagctcga
     3841 tctggtctat gcgaagtacg gcgagcgcga gctgcacctg gacctcttcc ggccgaagga
     3901 ccagggcgat cgcgcgctgc cggcggtgct ggtcgtgcat ggcggcggct ggctgcaggg
     3961 agataagacg cggtttaggg cgctggggca ggcgctggcg ctccgcggct atgtggcggc
     4021 cgtgatcgag tatcgcctgg gcggtgaagc caggttcccc gcggccgttc acgactgcaa
     4081 tgctgccatc cgctggctgc gggccaatgc gaagcaatac ggcatcgacc cggagcgcgt
     4141 cgccgcggtc ggcggatcgg ccggcgggca cctcgtcggc ctcgtcgcgg cggcgccgga
     4201 cgtgcgcgag ctgcaaggcg acggcggcaa cgccggccag tccagccgcc tgcaagccgc
     4261 cgtcgtgctg gccgggccga tggaactcgc caccggcccg gtggccgagc ggtcgcgcca
     4321 gaacccggaa atctcgaaca ccaaccgctg gctcggcaaa acaatcgacg aagcgcccga
     4381 gctctaccgg ctggcttcac ccttcacgca ctggtcgaag gccgcgccgc cgatcctctt
     4441 tatgcacggc gaacttgacc acccggagca aaacgccgcc tcgcgccgcc gcttgcaaga
     4501 gctgggcgtg ccggcgggcg tgctcgtcta tacggcgggc aagcacggct gctggaatca
     4561 gcacccctgg ttcgagccga tggtcgatga catcgacgcg ttcctgacga gcgccctcca
     4621 gcgcgacctc tatctgcagt ggttgcccaa gatcgaaacc gactggggcg agatgcgctt
     4681 cggcgcagaa tcggtcgatc tgctgatctt ccacaagccg gcgggcggcg tccttcaaat
     4741 cccgcggctg aacaatcccg tcaaggttgc ctacgtgcgc ggcgatgctg ctaagactcc
     4801 gctcaagctc tcgcccggcg tccgcgagtg gatgatcgac gtctccccgt tgcccggtgg
     4861 cgcgatcggg caaacggtca tcgtcgaaac ggtcggccgg ccgcatgtgc cgacgattcc
     4921 gcgtgtcgtt ggcggttccg ccgatggatc gattgtgctg cacgctcatg acgccgtcac
     4981 gcacggcgaa aacctccgct acgagccgca gccgcacaag aacacggtcg gctactggac
     5041 gaacgaggac gactggtgcg agtggcggtg ctacgtggag cagccgggga aatacgagtt
     5101 gcaaatccta caaggctgcg gcaaagggca aggcggcagc gaagtggaag tgcagattgg
     5161 cgatcaattc gtgacgttca cggtcgagga cacgggccac ttccagaact tcaaggagcg
     5221 gtcgctcggc accgtcacgc tcgctgcgcc gcaagttaac tcgctgcaga ttcgagccaa
     5281 aacgaaggcc gccgctgccg tgatggatgt gcggcaggtg cggctgattc ccgtggcgga
     5341 ctgacgtgcg agccgatcac gtcagcggca gcgggtggcc gtcgctgacg gcgaaggggc
     5401 ggccgagttg gtcgtggatc tccgcgcggg gcgagatgcc gagctgctgg aagatcgtgg
     5461 cgtgaatgtc gggcgggccg cacggctttt cgcgcgggaa ggcgccgttg ctgtcgctgc
     5521 tgccatagac ttgcccgccg cgaatgccgc cgccggccag cgcgaccgaa tagacgctcg
     5581 ggtaatggtc gcgccccgcc ccgccgttga tccgcggcgt gcggccgaaa tcggtcagca
     5641 tcacgaccag cgtttcgtcg agcgtgccgc ggtccgccag gtcttgcagc aaggccgaga
     5701 cgccgcgatc gaacaccggc agcaggcgat tcttcagccg gttgaagttg tcgctgtgcg
     5761 tatcccacat gtctcccgca ccgccgttga ggtcgccggc cgcacagcaa acctgcacga
     5821 ccggcacgcc cgcctcgatc agccggcggg ccagcagcat gctctgcccg ccgaggtgac
     5881 tgccgtacga ctcgcggatc tgcgggtcct cgcgatccag gtcgtatgca ttacgcaccg
     5941 cgctgccgag caaaatgtcg gtcgcaagct gccggaaatg gttgaagcca tcgaactcgt
     6001 cacggcgggg ctgcggcttg tcgagcgtgg cgagcagctc cttgcgggcg gcgacgcgat
     6061 cgaagtcgct gtttttgagc tccagcactt ccgagccaag cgtctgcgac acgccgccga
     6121 acggctcgcc ggcgggcggc cgcatgacga tcgggtcgta cttcacgccc agccagccgc
     6181 cgtactcgcc ggcttgcagc gtgtcgttat cgaccatcgg atacggaatg cggacggccg
     6241 gcggcacgcc cttgggcgcc cgccgcagct tggaaaccat cgccggcagc gaaggccaat
     6301 cgttcgggaa gggcggaatg ttgccggccc gcggcggcgc gtgtcccgtc aggttccagt
     6361 acatcccgcg accatgagcc gagtcgtcgt gaaagatcga gcggacgatc gccagcttct
     6421 cggtctgcct ggcgaggagc ggcaggtgct cgctgatctg aattcctggc gtggccgtcg
     6481 cgatcggcga aaactcaccg cgaatctcga ccggcgcgtc cggcttgagg tcccacgact
     6541 ccaaatggct catcccgccc caggtgtaga tgatgatgca ggatttggcc ttgccgctcg
     6601 gaacgccacg gagggcggtc cctacagtac cgtcgctcgt ctccgcagcg gcggcccgcg
     6661 tttgcaaaaa cgccggcagc gacagggcgc caagcccaac ggacgctgcc aacagcttcc
     6721 gccgggacag gcggcggatt gcgtccagag gaggcgaatt cggcacggga atcgccatgc
     6781 ggaatttcaa aacgtgaggc gggaggggaa aggcggcttt gcggtgggcg atactatcgt
     6841 aggcatcccg cgcggcgcga tgcaagccga ttttccgcgt gctgcttctg catctcaggc
     6901 cactgcccca ggcaaagcta gcgcggccgg ccgccaactt gcaccccact cgcgctgccg
     6961 gccagccaga cctcccttgg gacaaccgac gccggcctgc aagccgagca gggaattgaa
     7021 gtctcgtctc gtggaagcta atctgcccca catcgcttgg tttcatgttt catgtggggg
     7081 acctgcgatg ttgcgccaac ttggaattgc gttcggagtt tccttgctct tgctcaacgg
     7141 cgagatgctg actgcccagg agtcgtgttc gctgacgatt ccggtgaaag cggtcggtgc
     7201 gggcacgacc ttcttccaga cgttccagtt ccgcgaggga acgccgtcgc ttcccggtct
     7261 gttgtcggga aatgttgaga tcggcggcca ggtggtgcag gaagtgaaga tccgcatcgc
     7321 ccggcaagtg caacgggagg tggtcgagta tgtcgtggtt tatgtcgatg gacgtccgac
     7381 cgttcaacgg agggtcagcc tggtcgtcga aacggtttac gtctatgtcc ctgtgctgca
     7441 ggagatcgtt ctgcccgtat tcaccggcac ctggacttcc gtggatttgt gtttctactc
     7501 gacgtggaac attctccacg tcggaacgga tggcatggtc gttggcgtgg ggacaaccgt
     7561 tggcaaccag ttgtacggca gcgccctcta cttcggagtg gcaggcattc ccaatggcct
     7621 atattctctg tcaaccggag tgccgggcga cggcggcggc ggaggaggcg gcggtggtcc
     7681 cccggtcgaa ccttagtcgc cggtccgcgc cagcgcagtg ctcgattgat cgaggcgatt
     7741 gatcagcgcg acaacctcac ccggcagcgg ctggccgccg aggaggtcgc gcgactcggc
     7801 gcggaactga tcccatgcct cgccgccggc ctggcggcac gcgagagccg tcagcacgac
     7861 cgcgtcaggg ccggccacat gcttcgcgac cagttcacgc aacgactccg cgtcggttgc
     7921 ggccggattg cccacgagca tccgtccgag cagccgcagg tcggcggcgg aatcttcgcc
     7981 tgactgcacg gcgaacagct ctgcggcctt cccgcgaaga gtttcatcgc tgggatttgc
     8041 gagtactgca tacagcacgc tcgccagtgc cgcctgatcg ttcttgtcct tctcggcagc
     8101 ggccagcgcg gcctcggcag ccgccggcaa ttgcttccgg gcctctgctg ccaacgcagc
     8161 gagctccgtt tctcccggca gcgctttggc cgcctgcccg atcgtccata gcgaacgcat
     8221 cgcgagcggc cggccggcgc cgccgagcaa ttccgccgtc tgcgggctct tccaggcgat
     8281 ctgcagttcg gtcagcgctt ccagatcgag ctcgccgccc gcggccgcgg acaagagctg
     8341 ccaactgaac tggtcgtgcg ccgcggcgac gttcaacagc gcctcggcgg cggcagcttt
     8401 cgccacggac tcttttcgcg cggcatagcg cgtccgcgtc acttcgctga tcgcggacag
     8461 cgcgccggta ggcttcgttt tgccttcctg gagcgtggcg atcagtttcg tcacggcggc
     8521 gtgccggtcg ggctggccaa tcacgagcaa ctggcccgga gcgaaccagg tgacggaagt
     8581 ctcgtcgtcg gccttcagct ccttgcgcac cgcggcaagg aactcatccg ccgccttctt
     8641 tgcggcttcg accgccttgt tgtagtcgtt cagatcggtc agatctttct ccagcggcag
     8701 tgcgatcgcg ccgacgtcgt acgtccagcc gctctcgccc gctcgtcgcc gctcgctgcc
     8761 ggctacaacc gcctttccgt caacccgcca ggtgaggcgg gccggctgca ggatccaatc
     8821 gagggcctga ctggtggtgg cgtgccgcag gtcgagatag ctcacgcggg ccgcatcggg
     8881 gccaatgagc gccgtcgcat ccgccacgct cccttccagt aggttgatgt cgatcttcgc
     8941 cgccgtcgcg acctgatcga gcgcctcggc cagcgactga tcgcggatca ccaggtccag
     9001 cttcgtcttg agctgcggca gcttcgccga ccagtccttc acgtccgcgg cgtgcaactc
     9061 ctcgagcttc tcgatcgcct gaaaggcgat gccgccatcg gactggccca cgttcgccgc
     9121 agccgtgtcg tacaggcagg ctttcgcgac aaggcgctgt acctcgctcc ggtcggccgg
     9181 cgcttccttg cccgtcgcct tctcgaagag tgcccgggcc gactccacca gcttgccggt
     9241 ttgcggcgag atcgcattgg ccgtatagcc gaactcggac gccgtgatgc cgtcctttgc
     9301 ctccgcatcg gccaaggcct ccccgagggc caggcggtcg agttgcagcc gtccaccacg
     9361 atgtgtcggc gcggcatcaa aggactgtag gccgcgcccg cgggccagtc
//
LOCUS       DASUGB010000606         5137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_622871, whole genome shotgun sequence.
ACCESSION   DASUGB010000606 DASUGB010000000
VERSION     DASUGB010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622871"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1048)
                     /locus_tag="VFV87_11635"
     CDS             complement(269..1048)
                     /locus_tag="VFV87_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5104458.1"
                     /translation="MLIPYYQVDAFASRVFGGNPAGVCLLADWLPDDVLEAIAAENNL
                     AETAFVVQRAGRYDLRWCTPTMEVDLCGHATLAPAHVLFEHLGYQGETIRFDTRSGEL
                     TVTREGDLLVLDFPARPAAECPAPPELLAGLGRQPIYVGKARDYLAVFETEQEVAGLR
                     PDFVALAKLDSLGIIATAPGATCDFVSRFFAPQAGIPEDPVTGSAHSTLIPYWSQRVG
                     KKKLHARQISTRGGELFCDDRGPRVGIGGRAVTYSTGFVRL"
     gene            1111..2421
                     /locus_tag="VFV87_11640"
     CDS             1111..2421
                     /locus_tag="VFV87_11640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5104459.1"
                     /translation="MSTITSRRQDVAPPAEVQPALTFLRELSQGFESRDFAVRFWNGW
                     TWEPDAGQEAKCTLVLKHPGAVRQMFWRPNKAAFGEAYIYDDFDIEGDVLAIFRWIRY
                     LQLHKPAGLNYLRKAAQILRMPNQSTRRQGRGSAALAGSQHSESRDKQAIQYHYDEIP
                     SEFYAVILDKYMQYTCGYFASPDDDIDTAQERKLDYICRKLRLRPGERLIDFGCGWGG
                     LITFAAKHYGVQAVGVTISKEQMAWATRLIEQEGVGDRCRLEFCDYRKVPEAEKYDKA
                     VSVGFIEHLGERMMPTFFGKVRRLLRPNGLYLHHGITLKPFSPYPDWRAFTVKYVFPD
                     GELVPINRTVDQLAKAGFEVRDVESLREHYMLTLDRWLRRLEASADQVRELTDEVTYR
                     IFRIYLAGALSGFRWGIYNLHQTLVVNSGEDVSGLPLARADWYV"
     gene            2568..3887
                     /locus_tag="VFV87_11645"
     CDS             2568..3887
                     /locus_tag="VFV87_11645"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX5104460.1"
                     /translation="MIPRLVSLDAYRGFVMLAMVSHSLGLPAVVRELANRHDQHLAFW
                     QQLAYQFDHVQWPGCVFWDLIQPSFMFMVGAAMAFSCAARRAKGQSYWVLAVHAAWRS
                     VALVLLGVFLRTEIKPDPDGGWPITNFTFVDVVSQIGLGYFFLFLLWGRRQWVLMLAA
                     VSILVSYWALFAAYPLPTPDFKYERWGVPEDWEYLYPETDFQAHWNKNSNPASAFDRW
                     FLNKFPQRDQEVPGGPLPPRRGSGFQFNPGGYATLNFIPSLATMIFGLMAGQMLLGPA
                     TYLAPRHELPHSQRDAHVRSSREKFGLLLLWGLAGLVIGYLLHISGVCPAVKRIWTPT
                     WTIYSTGWTLIVLAGFYGIIELANFRWWAFPLAVVGMNSIAIYCLDKLTTPWIVARLK
                     VHFGGDIFSLWGLVDSAYAPLVQSLFVMAILWLVMYWLYRRKIFLKI"
     gene            3945..>5137
                     /locus_tag="VFV87_11650"
     CDS             3945..>5137
                     /locus_tag="VFV87_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104461.1"
                     /translation="MAEIEFLDPQVVDAEVVAPAGQPCEACATPMEPLDRFCPACGTP
                     NPHFRPPLAGKDATDAGTSGPSKQAVDAGGQGRGAAVVAAQLVEPSPLARHFECKTCG
                     AQVATEPSQRSYVCPFCDSTYVQELPPEKTGRQRPEFVIGFAITPDQAHAEFKKWLGR
                     NSWYRPGDLAVAASVADKLKGIYLPFWSFSMLAQSAWRAQIGEYWYRTETYTTTDSKG
                     NTTTHTRQVRETEWWPCGGRHHRYYSGYLVSGSKGLPQSQSLRIQPFNLPALKRYEPY
                     FLAGWLAEEYSIARDEALVLCQQEFQRQENANVARFLPGDTHSSLEVQTQFSQVNSDL
                     CLLPIYILSYRYKEKVYRYLLNGQTGKMDGDKPVSGQRIGIAVGVALAIVLLIVLIII
                     ILANM"
BASE COUNT          909 a         1631 c         1636 g          961 t
ORIGIN      
        1 cgggatcgtg cagctcatag ttgaggggtt gggattcggt cagggccaag gcatcggctc
       61 gatatgcaat tcgccgcggg tcgtctctcc ctaagtctaa gccgagtcag cacatgggac
      121 cagccgccgc cgtgcgcctc gaaggctgtg cgagcgcggt tgatccctgt ttctaacccc
      181 ggcagagggc gggggtttcg atagcagagt gccgcggtag cgccccttcg tttaacgcgc
      241 agccgcaggc gatgccgtgc aatcagcact acaagcgaac gaagccggtc gagtaggtca
      301 ccgctctccc gccgatgccg acgcgcgggc cgcgatcgtc acagaaaagt tcgccgccgc
      361 gggtagagat ctgccgggcg tgcagctttt tcttgccgac gcgctgcgac caatagggga
      421 tcagcgtcga gtgcgccgaa ccggtgaccg gatcttcggg aatgcccgcc tgcggggcga
      481 agaaacggct gacgaaatcg caggttgcgc cgggagcggt ggcgatgatt ccgaggctgt
      541 cgagcttcgc gagggccacg aagtcgggcc tgaggccggc gacctcctgc tctgtttcga
      601 agaccgccag atagtcacgc gccttgccca catagatggg ttggcggccg aggccggcga
      661 gcaattccgg aggcgctggg cactcggctg cgggccgggc aggaaaatcg agcaccagca
      721 agtccccctc gcgcgtcacc gtcaattcgc cggagcgcgt gtcgaagcgg atcgtctcgc
      781 cctggtatcc cagatgctcg aagagcacgt gcgccggcgc cagcgtcgca tgcccgcaca
      841 gatcgacctc catcgtcggc gtacaccaac gcaggtcgta ccgcccggct cgctgcacga
      901 caaacgccgt ctccgccagg ttgttctccg ccgcaatcgc ctccagcacg tcatccggca
      961 gccaatcggc cagcaggcac acgcccgccg ggttgccgcc aaacacgcgg ctggcgaacg
     1021 cgtcgacctg gtagtaaggg atgagcatcg ctttgtcctg aggctccgtg cgaaggttat
     1081 aatcgccgct gaccacggag caagcaacgc atgtccacga tcaccagccg ccgacaagac
     1141 gtagcgcccc ccgccgaggt tcagccggcc ctgacatttt tgcgcgaact ctcgcagggc
     1201 ttcgaatcgc gcgatttcgc cgtccgcttc tggaacggct ggacgtggga gccggacgcc
     1261 gggcaagagg cgaagtgcac gctggtcctt aagcatcccg gtgcggtgcg gcagatgttc
     1321 tggcgtccga acaaagccgc cttcggcgag gcgtatatct acgacgattt cgacatcgaa
     1381 ggggacgtgc tggccatctt tcgctggatt cgctacctgc agcttcacaa gcccgccggc
     1441 ctcaactacc tgcgtaaggc cgcgcagatt ctccgcatgc ccaatcaaag cacgcggcgg
     1501 cagggacgcg gcagcgctgc gctggccggc tcgcagcact ctgaatcccg cgacaagcag
     1561 gcaatccaat accactacga tgaaatcccc agcgagttct acgccgtcat tctcgacaag
     1621 tacatgcagt acacctgcgg gtactttgcc agccccgatg acgatatcga cacggcgcag
     1681 gaacgcaagc tcgactacat ctgccgcaag ctgcgcctga ggcccggcga gcgactgatc
     1741 gattttggct gcggctgggg cgggctgatt acgttcgccg cgaagcacta cggcgtccag
     1801 gcggtcggcg tgacgatcag caaagagcag atggcgtggg ccacgcggct gatcgagcag
     1861 gagggtgtcg gcgaccgctg ccggctggag ttctgcgact atcgaaaggt gccggaagca
     1921 gagaaatacg acaaggcggt cagcgtcggc ttcatcgagc acctgggcga gcggatgatg
     1981 ccaacgttct tcggcaaagt ccgccggctc ctgcggccca acggcttgta cctgcatcac
     2041 ggcatcacgc tgaagccgtt ctcgccctat cccgattggc gggccttcac cgtcaagtat
     2101 gtcttccccg acggcgagct cgtgccgatc aaccgcaccg tcgatcagct cgccaaagcc
     2161 ggcttcgagg tccgcgacgt cgaaagcctc cgcgagcatt acatgctcac gctggatcgc
     2221 tggctgcggc ggctggaagc ctcggccgat caggtcaggg agctgaccga cgaagtgacg
     2281 taccgcatct tccgcatcta tctggccggg gccctcagcg gcttcaggtg ggggatctac
     2341 aacctgcacc agacgctggt cgtcaactcc ggcgaggacg tctccggcct gccactcgcc
     2401 cgggcggatt ggtacgtcta gcggtggaag cctacttcgc tttggctagc acgggaagaa
     2461 gaggcgcggc ggtaatgtcg ctctgcggag cgtgacggcc tggccggccg ccgggtacaa
     2521 tgctccccat ggccgagagc caatctaact ctgctgcgac ggcttccgtg attccccggc
     2581 tggtgtcgct ggatgcttat cgcgggtttg taatgctggc gatggtgtcg cacagcttgg
     2641 gattgcctgc cgtcgttcgc gaactggcga accgtcacga tcagcatctc gcgttctggc
     2701 agcaactcgc ctatcagttc gaccacgttc agtggccggg ctgcgtcttc tgggacctga
     2761 ttcaaccgtc gttcatgttc atggtcggcg cggcgatggc gttctcgtgt gcagcccggc
     2821 gggcgaaggg gcaatcctac tgggttctgg ctgtccacgc ggcgtggcgt tcggtggcgc
     2881 tggtcctcct gggggtgttc ctgcgaacgg agatcaagcc ggacccggac ggcggctggc
     2941 cgatcaccaa cttcacgttc gttgatgtcg tgtcgcaaat cggcctcggc tatttctttc
     3001 tgtttctctt gtggggccgc cgccaatggg tcctgatgct ggcggctgta tccatactcg
     3061 taagctattg ggccctcttc gccgcctatc ccttgccaac accggacttc aaatatgaac
     3121 gatggggagt tcccgaggat tgggaatact tgtatccgga aacggatttt caggcgcact
     3181 ggaacaagaa ctcgaatcct gcctccgcct ttgaccgctg gttcttgaac aagtttccgc
     3241 agcgcgatca ggaagttccc ggggggccgc tgcccccgcg aaggggttcg ggatttcagt
     3301 tcaatcccgg cgggtatgcc acactgaatt tcatcccctc gctggcgaca atgatcttcg
     3361 gcctgatggc ggggcagatg ctgctggggc ctgccaccta tctcgccccg cggcacgagc
     3421 tgccgcattc gcagcgcgac gcgcacgtcc gttccagccg cgagaaattc gggctgctac
     3481 tattgtgggg cctggcgggg ctggtgatcg gctacttgct gcacatcagc ggcgtctgcc
     3541 ccgcggtcaa gcggatctgg acgccgacct ggacgatcta cagcaccggc tggacgctga
     3601 tcgtgctggc gggcttttac ggcatcatcg agctggccaa ctttcgctgg tgggcgtttc
     3661 cgctggcggt ggtcggcatg aactcgatcg ccatctattg cctcgacaag ctgacgacgc
     3721 cgtggatcgt cgcccgcctc aaggtccatt ttggcggcga catcttctcg ctgtgggggc
     3781 tggtcgattc ggcctatgca ccgctggtgc agagcttgtt cgtgatggca atcctctggc
     3841 tggtgatgta ctggttgtac cggcggaaga ttttcttgaa aatctgacct gtcattgctt
     3901 cttcgcacct tccgtgctgc ttcccgcgca cgaagcgccc tacgatggcc gagatcgagt
     3961 ttctggatcc gcaggttgtg gatgccgagg tcgtcgcgcc cgccggccaa ccgtgcgagg
     4021 cgtgcgcgac gccgatggag ccgctggacc ggttctgccc cgcttgcggc acgccgaatc
     4081 cgcatttccg gccgccgctc gcggggaaag acgccaccga cgccggaaca tccggaccaa
     4141 gcaaacaagc cgtcgatgcg ggagggcagg gcaggggggc cgcggtcgtc gcagcccaac
     4201 tggtcgagcc ctcgccgctg gcacggcatt tcgagtgcaa aacgtgcggg gcgcaggtcg
     4261 cgaccgagcc gagccagcgg tcgtatgtct gcccgttctg cgattcgacc tacgtccagg
     4321 agttgccgcc ggaaaagacc ggccgccagc ggccggagtt tgtcatcggc tttgcgatca
     4381 cacccgacca ggcgcacgcg gagttcaaaa agtggctcgg gcggaatagc tggtaccggc
     4441 ccggagatct ggcggtggcc gcgtcggtcg ccgacaagct caaaggcatc tacctgccgt
     4501 tctggtcgtt ctcgatgctc gcccagagtg cctggcgggc gcagatcggc gaatactggt
     4561 accgcaccga gacctacacg acaaccgatt ccaaagggaa caccacgacc cacacgcgcc
     4621 aggtccgcga gaccgaatgg tggccctgcg gcggccggca tcaccgctat tactccggct
     4681 acctcgtcag cggcagcaag ggcttgccgc aaagccagtc gctgcggatc cagcccttca
     4741 atctgccggc cctcaagcgg tatgagccgt atttcctcgc cggctggctg gcggaggaat
     4801 attcgatcgc ccgggacgaa gcactggtgc tctgccagca ggaattccag cgacaggaga
     4861 acgccaacgt cgcccgcttc ctgccgggcg acacgcacag cagcctggag gtgcagacgc
     4921 agttcagcca ggtcaactcc gacctgtgcc tcttgccgat ctacatcctc agctatcgct
     4981 acaaggagaa ggtttaccgg tacctgctca acggccagac cggcaagatg gacggcgaca
     5041 agccggtgag cggccagcgg atcgggatcg cggtgggtgt cgccttggcg attgttctcc
     5101 tgattgtgtt gatcatcatc atcctggcga acatgca
//
LOCUS       DASUGB010000607         7961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2013669, whole genome shotgun sequence.
ACCESSION   DASUGB010000607 DASUGB010000000
VERSION     DASUGB010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013669"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFV87_11655"
     CDS             complement(<1..485)
                     /locus_tag="VFV87_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104462.1"
                     /translation="MSVGSRWFALLALAAFTVPLFAADWPQWRGPNRDDVSTETNLQP
                     RWPADGPSRLWLSDQAGIGYSGFSVVGDTLYTMGADDQTESVIAINVANGDVKWSTPI
                     SERLKNGYGDGPRCTPTIEGHRIYALGGRGALACLNTADGAEIWTVQMRDFGGRTPGW
                     G"
     gene            complement(615..1481)
                     /locus_tag="VFV87_11660"
     CDS             complement(615..1481)
                     /locus_tag="VFV87_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5104463.1"
                     /translation="MFSSLSRRRFLSLSAAAAAGATWFDTPRVLRAAGLADEKDPFGG
                     FPVGVQSYSLRNFETPEVIRHLQGMGVHYTEFYGKHLDPKASDEQIAETLKLLQSANI
                     KLAGHGVHGFSKNHEANKALFDFAKKAGVKVITANPEKDAFESLDKLVAEYDIRIAIH
                     NHGPNALYDKLSDVTQAVKGHDKRIGACVDCGHYLRSGEDPVKCVLELGDRVYGVHIK
                     DEKETNTPKSANVVIGKGHLDVVGLFKALKQIKFPADGALSLEYEANPMNPIDDMKAC
                     LEVAKEAIAKVA"
     gene            1628..4936
                     /locus_tag="VFV87_11665"
     CDS             1628..4936
                     /locus_tag="VFV87_11665"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX5104464.1"
                     /translation="MTNSIRNPQSAIRNWPLSLCISLAASAALAQPDPSPPEMREDAE
                     LTAVTFLNADRGWAVGDRGVIWHTIDGGRSWKQQESGVTCRLESVQFLDSDNGWAAGG
                     WTQPYTHETHGVVLRTRDGGKTWQNTPGLTLPGLARLKMLDPKWGWAVGSRSHLYPSG
                     AFFTDDGGRTWTPVVKGETAGWVTGDFADHRGGVVVGLDGSIALVTAKELRGIHSPNT
                     GLRLVRRLLLAGSKLGPGELPVDPARDAPGPGLVPAKKAEQGWLVGDGGLVLTTADGG
                     ITWSEPAGKVPEFTAANLDFRALAAFGSHIWIAGAPGTCVLHSADGGQSWDCFRTEQT
                     APLAGLCFIDEYRGWAVGALGTILHTRDSGQTWRLQHSGGKRVGMLGVFAEPERIPLE
                     LIGLVSGSDAYLSAIEIIGRQPDDLTAPARTHAALLAAGGSHADTAWQFPLAERGLPQ
                     SPASILARWNALCEGRATERLEEHIVRRIRQWQPEVIVTEDVSPRGDDPLAHLTNQIT
                     LAAVQKAGELTAYPDQVTHAGLLPWRVKKVLTLVPGAKPGLINITPAQWAPRIGRSLA
                     DVAEGGRGLIRSDVVPAARNIGLSLLVDHLPQETGRRDVMSGITLDPGSEARRQLSHP
                     PAGDLEQLGRLAQKRHNVEQLLARIDGGGANLGNWLGQVDELTRGLPARQSGEILWQL
                     GQRYHQAGRGDSAAEALELLVQKHPQHPLADRAALWLVQYYASGEMAWRERKQNGANF
                     GVAAASQPADSPTAVKPAAATQAVAIAAPIQASFDSMQRMGHVSSNMTPAQRSGRALA
                     LANQIQRTRPTLYADPALRFPLAVAMRGSEPGDSSPLSPRGRGAGGEGARLLQTLPQL
                     GPANPWTANLAAEEWLVSSRADAPKKLLSVVSASEKPKLDGRLDDALWQSAKAVSLTP
                     TRRASEATATRSVSEATVTRSVSEGGHQPTPEPADLFTAAVLAFDDEFLYVAVSCQKA
                     PGCEYPADSKPRIADEDLSQTDHVTLLLDIDRDYTTYWRLSFDHRGRPAESCFGDATW
                     NPQWYVAAAGDETYWTVEAAIPLAELAERKPEVRDVWSANIQRVIPGIGLQSSSQPAG
                     IEIRPEGFGLLVFE"
     gene            complement(4952..6508)
                     /locus_tag="VFV87_11670"
     CDS             complement(4952..6508)
                     /locus_tag="VFV87_11670"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5104465.1"
                     /translation="MRTKIRGRCVLMLALLAAVASLPGRLSAQDAAAVIAAGKALFER
                     QFVAGEPINAGGDGLGPVFNHVSCLACHHQGGAGGSGPVDVNASMLSVDLIKLETAPE
                     RSEFRRALAAIHPGFISADGELASSVILHRFGSDARYNAIRQKFLGAAVPLRPDEDER
                     GELQGTLARAPAQPVKAARPLTLVVTQRNTTALFGAGLIDQIPDATLHAIAALQSKHP
                     EVSGRVAPIDNTKVGRFGWRGQIEHLHEFNLGACSNELGLQVPTKSQPMDPLRPEYKP
                     SGLDLTIPQCLSLTAFVASLPAPKVVRPNDPDKLRAVEHGYKVFQAVGCVVCHVETVG
                     SAQGIYSDLLLHDLGPSLADPVEAQPVFVMVEQVQIPIESAHDQDGTIVENRLSVSSS
                     TTTTSNTYYGRTTTMTSTARSILGQGVFYKTQRGFMTVVPDPKQQAYGIRTKYEPLPT
                     NLTQEWRTPPLWGLQDSAPYLHDGRAATVIEAIALHGGEAEACTKRYLGLPAGDRLAM
                     LAFLECLRAP"
     gene            complement(6492..7739)
                     /locus_tag="VFV87_11675"
     CDS             complement(6492..7739)
                     /locus_tag="VFV87_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104466.1"
                     /translation="MGWLLISLSANAEEVPRWLDNRAEAAQQARKEGKLLVVVELSGD
                     FARNAADARELQLYRSLAAADPRIAAEFRSRYVVARQHVGEAESLRKLPTAPSRSKAA
                     AAERTDYAITYICLPDERVLHFVPGFVTSDELLAELAWAEKCYAKVAAVASAGEELAA
                     RQAHQAAAAKSDLALFHKRFPSRWSGEALAEGPSTVDLPMGLVAARDTVADSLAQRLG
                     GSWSRRDLPALLAALSAHGALGREIAHLLLAEFPLVALGDVERPAFEACSGQRFWEAS
                     SRREKLAQWWKESRAGGKPVLLVVADDPHAVAAASASAFMWPPENTELVPQLPLFASE
                     LVSIDELAALATDASLPAMTYRAGQAPPRYLVFSADGKPAVHLGKSTNLTRLSQALEA
                     ATGAGALATVASKREGESDADED"
BASE COUNT         1417 a         2627 c         2543 g         1374 t
ORIGIN      
        1 tatccccagc cgggagtgcg gccgccaaag tcgcgcatct gcaccgtcca tatttccgct
       61 ccgtcagccg tattcaagca agcgagtgcc ccgcgtccgc cgagtgcgta gatgcgatgt
      121 ccttcgattg tcggagtgca gcgcggaccg tcgccatagc cgttcttgag tcgttcgctg
      181 attggtgtgg accacttaac gtcgccattg gcgacgttga tcgcgatgac cgattccgtt
      241 tgatcatcgg ctcccatcgt gtagagcgtg tcgccgacaa ccgaaaatcc cgaatagccg
      301 atgcctgcct gatccgacag ccaaaggcgc gaaggtccgt ctgccggcca tcgtggttgc
      361 agattcgtct ccgtggatac gtcgtcccgg ttgggccccc gccactgcgg ccaatcggca
      421 gcgaatagcg gaactgtaaa cgcggctagg gcaagcaatg caaaccagcg ggaaccaacg
      481 gacatcggtg agactccagg cgggagagtt ttggtgggtg tgaaccttta tcgtagcgtg
      541 tgacccagga aacttcgagt cgaatgcgct gctcgctgac accccaatgc tggctcctgg
      601 ctccgatttc tgcgtcacgc caccttcgcg atcgcctctt tggcgacttc caggcaggcc
      661 ttcatgtcgt cgatggggtt catggggttg gcttcgtatt caagcgaaag ggcgccgtcg
      721 gccgggaatt tgatctgctt gagggccttg aagaggccga ccacgtcgag atggcccttg
      781 ccgataacga cgttggccga tttgggcgtg ttggtctcct tttcgtcctt gatgtggacg
      841 ccatagacgc gatcgcccag ctccagcacg cacttgaccg ggtcttcgcc gctgcggagg
      901 tagtggccgc aatcgacgca cgccccgatt cgtttgtcgt ggcctttgac cgcctgggtc
      961 acgtcgctca gcttgtcgta gagcgcgttg ggaccgtgat tgtggatcgc gatgcgaatg
     1021 tcatactcgg caaccagctt gtcgaggctc tcgaaggcgt ccttctcagg attggccgtg
     1081 atgaccttca caccagcctt cttcgcaaag tcgaacaacg ccttgttcgc ttcgtgattc
     1141 ttcgagaagc cgtggacgcc gtggccggcc agcttgatat tggctgactg cagcaatttc
     1201 agcgtctcgg cgatttgctc atcgctggcc ttgggatcga ggtgcttgcc atagaactcg
     1261 gtgtaatgca cgcccatgcc ttgcaggtgg cggatcacct caggcgtctc gaaattccgc
     1321 agcgaatagc tctgcacgcc gaccggaaag ccgccgaagg gatccttctc gtccgccagg
     1381 ccagccgccc gcagaacccg cggcgtatcg aaccacgtcg cccccgcggc agcagcggca
     1441 gaaagggaaa ggaaacgacg gcgggagagg ctggagaaca tgggcaaggt ccttgtgcgg
     1501 gtgcttgtgc agcagaaagg cagattatgt acgcagattc gcgcggtcgc aatgatcggc
     1561 ctggagacgg tcttgggcga ctcgctatca tccgcccgcg ccccgaatga ccaatgacca
     1621 ggctcccatg accaattcaa ttcgcaatcc gcaatccgca atccgcaatt ggccgctctc
     1681 actttgcatt tctctcgcgg cttccgcggc cctggcccag cccgaccctt caccgcccga
     1741 aatgcgcgag gacgccgagc taaccgcggt cacgttcctc aatgccgatc gcggctgggc
     1801 cgtgggcgac cgcggggtga tctggcacac gatcgatggc ggccgaagct ggaagcagca
     1861 ggaatccggc gtcacttgcc gcctggaatc ggtgcaattc ctcgacagcg acaatggctg
     1921 ggcggcgggg gggtggacgc agccgtacac gcacgaaaca cacggcgttg tgctccgcac
     1981 gcgggacggc ggcaaaacgt ggcagaacac gccaggactg acgctacccg gcctggcccg
     2041 actgaagatg ctcgatccca agtggggctg ggccgtcggc agccgatcgc acctatatcc
     2101 gtcgggggcc tttttcaccg acgacggcgg ccgcacttgg acgcccgtcg tgaagggcga
     2161 aaccgccggc tgggtgactg gcgattttgc cgatcaccgc ggcggcgttg tcgtgggctt
     2221 ggatggcagc atcgcattgg tcacggccaa ggaactgcgg ggaattcact cgccgaatac
     2281 agggctgcga ttggtgcgca gacttctttt agccgggagc aaacttggtc ccggggaact
     2341 gccggttgat cctgctcgcg acgcgccggg accaggtttg gtcccggcta agaaagcaga
     2401 gcagggctgg ctggtgggcg atggcggcct cgtgctgacg accgccgacg gcggcatcac
     2461 ctggagcgag cccgccggca aggtccccga attcaccgcc gccaatctcg atttccgcgc
     2521 gctggccgcc ttcggcagcc acatctggat cgccggcgcg ccaggcacct gcgtgttgca
     2581 ctcggcggac ggcggtcagt cgtgggattg cttccgcacc gagcagaccg cgccgcttgc
     2641 cggcctctgc ttcatcgacg aataccgcgg ctgggcggtc ggagcgctcg gcacgattct
     2701 gcacacccgc gacagcggcc agacctggcg cctgcagcac agcggcggca aacgggtcgg
     2761 catgctcggt gtattcgccg aaccggagcg cattccgctg gagttgatcg gactcgtctc
     2821 gggcagcgac gcctatctat cggccatcga aatcatcggc cgccaaccgg acgacctgac
     2881 cgcgcccgcg cgaacgcatg ccgccctgct cgctgccggt ggctcgcacg ccgacacggc
     2941 gtggcaattc ccgttggccg agcgcggctt gccccagtcc ccggcctcga ttcttgcccg
     3001 ctggaacgcg ctgtgcgaag ggcgcgcgac cgagcggctg gaggagcaca tcgtccgccg
     3061 aattcgccag tggcagcccg aggtgatcgt cacggaggac gtcagccccc gtggcgacga
     3121 cccgctggcg catctcacga atcaaataac actcgccgcg gtgcagaagg ccggcgaact
     3181 gaccgcctat cccgaccagg tcacgcacgc cggcttgctg ccgtggaggg tgaagaaggt
     3241 cctgacgctg gtgccgggcg caaagccagg gctcatcaat atcacacccg cccagtgggc
     3301 gccgcgaatc ggccggtcgc tggccgatgt ggccgaaggc ggccgcggct tgatccgcag
     3361 cgatgtggtt ccagcagcac gaaacatcgg cctctcgctc ctggtcgatc acctgccgca
     3421 agagaccggc cgccgcgatg tgatgagcgg catcacgctc gatccgggga gcgaagcccg
     3481 gcggcagctt tcccatccgc cggcgggcga tctggaacag cttggccgcc tggctcagaa
     3541 gcggcacaac gtcgagcagc ttctggcccg catcgacggt gggggggcaa acttgggtaa
     3601 ctggctgggc caggtcgatg agctgacccg gggccttccc gcgcggcaaa gcggcgagat
     3661 tctgtggcag ctcgggcagc ggtaccacca ggccggccgc ggcgactccg ccgccgaggc
     3721 cctggaactg ctcgtgcaga agcatccgca gcatccgctg gccgatcgcg cggcgctgtg
     3781 gctcgttcag tattacgcca gcggtgagat ggcttggcgc gagcggaagc agaacggcgc
     3841 gaattttgga gtggccgccg cgtcacaacc cgccgattcg cccaccgcgg tcaaaccggc
     3901 ggccgccact caagccgtcg ccatcgccgc gccgattcag gccagctttg acagcatgca
     3961 gcgaatgggc cacgtttcgt ccaatatgac ccccgcgcag cgctctggtc gggcgctcgc
     4021 cctggccaat caaatccagc ggaccaggcc cacgctctat gccgatccgg cactgcggtt
     4081 tccgctcgcc gtggcgatgc gcggcagcga gcctggtgat tcctctcccc tctcccctcg
     4141 tgggagaggg gccgggggtg agggggcgcg cctcctgcag acgctgccgc agcttgggcc
     4201 ggccaatccc tggaccgcga acctcgctgc cgaggagtgg cttgtctcca gcagggcgga
     4261 tgcacccaag aagctgctct cggttgtctc ggccagcgaa aagcccaaac tcgacggccg
     4321 tctggacgac gccctctggc aatccgccaa ggcggtttcc ctcacaccaa cccgacgcgc
     4381 aagcgaggca accgcaaccc gcagcgtcag cgaggcaacc gtaacccgaa gcgtcagcga
     4441 gggaggccac cagccgacgc ccgagcccgc cgacctcttc accgccgccg tcctcgcctt
     4501 cgacgacgag ttcctctacg tcgccgtgag ttgccagaag gcccccggct gcgagtaccc
     4561 agccgactcc aaaccgcgaa tcgccgacga agacctctcg cagaccgacc acgtcacgct
     4621 gctcttagat atcgaccgcg actacacgac ctactggcgg ctgtcgttcg atcaccgcgg
     4681 ccggccggcg gaaagctgct ttggcgacgc cacgtggaac ccgcaatggt acgtggctgc
     4741 cgccggcgac gaaacgtact ggaccgtcga agccgcaatt ccgctggcgg agttggcaga
     4801 gcgaaagccg gaggtccgcg acgtctggtc tgccaacatc cagcgcgtca ttcccggcat
     4861 cggcctgcaa tcgtccagcc aacccgcggg catcgaaatc cgccccgaag gctttggatt
     4921 gctcgtcttc gagtgaattc tcgtcccctg cctacggcgc tcgcaggcat tcgagaaacg
     4981 cgagcatcgc cagcctgtcg ccggccggaa gaccgaggta gcgtttggtg caagcttcgg
     5041 cttcgccgcc gtggagagca atggcttcga tcacggtcgc cgcccggccg tcgtgcaagt
     5101 agggcgcaga atcctgcagc ccccacagcg gcggcgtccg ccactcctgt gtaaggttgg
     5161 tgggaagggg ctcatatttc gtgcgaattc cgtaggcctg ttgtttcggg tctggcacaa
     5221 cggtcatgaa accgcgctgg gttttataga acacaccttg ccccaagatg ctgcgcgccg
     5281 tcgaagtcat cgtcgttgtc cggccatagt aggtatttga agtggtggtc gtcgagctac
     5341 tcacactcag tcggttctcg acgatcgtgc cgtcctggtc gtgcgcgctt tcgatcggta
     5401 tctgcacttg ctctaccatc acgaagaccg gctgagcttc caccggatcg gccagcgacg
     5461 gccccagatc gtgcagcaac aggtcgctgt aaatgccctg cgccgagccg accgtttcta
     5521 catggcacac cacgcagccg acggcctgaa aaaccttgta gccgtgctcg accgccctca
     5581 gcttgtcggg atcatttggt ctgacgactt tgggagcagg cagagaggct acaaacgccg
     5641 tcagcgacag gcattgcggg atcgtcaggt ccagcccgct cggcttgtat tcgggacgta
     5701 aagggtccat cggctgggac ttggtcggga cttgcagtcc caattcattg gaacaagcgc
     5761 cgaggttgaa ttcgtgcaga tgctcgatct gcccgcgcca gccgaagcgg ccgaccttcg
     5821 tgttatcgat cggcgcgacg cggccggaga cctccggatg cttgctctgc agcgcggcta
     5881 tcgcgtgcaa cgtggcatcg ggaatctggt cgatcaagcc ggccccgaaa agggcggtcg
     5941 tattccgctg ggtgacgacc agcgtcaaag gacgggcagc cttcacgggc tgcgcgggcg
     6001 cccgggccag cgtcccttgt agttcgccgc gctcgtcctc gtccggccgc aggggcactg
     6061 ccgcacccag gaatttctgc cggatggcgt tatagcgcgc gtcgcttccg aagcgatgaa
     6121 ggatcacgct cgaggcaagc tcgccgtcgg ccgagataaa gcccggatgg atggccgcca
     6181 gcgcccggcg gaactcgctg cgctccgggg cggtttcgag cttgatcaga tcgaccgaga
     6241 gcatcgacgc gttgacatcg accggccccg agccgccggc gcctccctgg tggtggcagg
     6301 cgagacacga aacgtggttg aagacggggc ccagcccgtc accgcctgcg ttgatcggct
     6361 cgccggcgac aaattgccgt tcaaagagcg ccttgccggc ggcgatgaca gccgcggcat
     6421 cctgggccga taaccggccc ggcaagctgg ccacggccgc cagcagcgca agcatcagaa
     6481 cgcaccgccc tctaatcttc gtccgcatcg ctctctcctt cacgtttgct ggccacggtg
     6541 gccaaagccc ctgcaccggt tgcagcctcc agcgcctgcg agaggcgcgt caggttggtt
     6601 gatttgccaa ggtgaacggc tggtttgccg tccgcgctga atacgaggta acgaggcggc
     6661 gcctgcccgg cgcgataggt catggccggc agcgaggcat ccgtcgccaa agcggccaac
     6721 tcgtcgatcg acaccagctc actcgcaaag agcggcagtt gtggtacgag ctcagtgttt
     6781 tcgggcggcc acatgaaggc gctggcggac gcagccgcca cggcatgcgg gtcgtcggcg
     6841 acgacgagca agacgggctt gccaccggcc cgcgactcct tccaccactg cgccagcttt
     6901 tcgcgccggc tggaagcctc ccagaaccgc tggccgctgc acgcctcaaa ggcggggcgc
     6961 tccacgtcgc ccagcgcaac cagcgggaat tccgccagca gcaagtgggc gatttctcgc
     7021 cccagcgcgc catgcgcgga gagcgccgcc aaaagcgccg gcaagtcccg ccgcgaccaa
     7081 cttcctccca accgctgcgc caggctatcc gcgactgtat cgcgagccgc gaccagcccc
     7141 atcggcagat caacggtcga aggaccctcg gccagcgctt cgcccgacca ccgcgaggga
     7201 aatcgtttat ggaagagcgc caggtcggat ttggcggcgg ctgcctggtg cgcctgtcgt
     7261 gcggccagtt cctcgccggc agaggcgacc gcagcaacct tggcatagca tttttccgcc
     7321 caggccagtt cggccagcaa ctcgtcggac gtgacgaaac ccggaacaaa gtgcaagacc
     7381 cgctcgtcgg gcaggcagat gtacgtaatg gcgtaatcgg tccgctcggc tgctgcggct
     7441 ttcgaccgcg atggcgctgt tggtagcttc cgcagcgatt ccgcttcgcc cacgtgctgc
     7501 cgcgcgacga cgtagcggct gcggaattcc gcggcaatgc gcggatcggc cgcggcaagg
     7561 ctgcggtaaa gctgcagttc gcgggcgtcc gcggcgtttc gggcaaaatc acccgaaagc
     7621 tcgacgacga ccagcagctt gccttccttg cgagcctgtt gggccgcttc ggcgcggtta
     7681 tcgagccacc gcggcacctc ctccgcgtta gccgatagcg agatcaacaa ccaacccatc
     7741 gcagcgcaga gggcgagcgc tatggatcga tgatgaggaa cagcgactgg caccgcttcc
     7801 tcgctggagt tttgcccctg aaaggccaca cccgcgggaa agagtgcatg gccgcgaggc
     7861 gtgtccaacg ccgttaacct aacgattgcc ggggcaaagt caatcgaatt cctgacttga
     7921 ggcgtagggt gggaccagcg agcaaagcga gcgcaggccc a
//
LOCUS       DASUGB010000608         4458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1736132, whole genome shotgun sequence.
ACCESSION   DASUGB010000608 DASUGB010000000
VERSION     DASUGB010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736132"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1411
                     /locus_tag="VFV87_11680"
     CDS             326..1411
                     /locus_tag="VFV87_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104467.1"
                     /translation="MLAKAAPAIRLIDAAQAKPKCVFITGLGIDALLPHAQAARTVTR
                     LSVLQLQQARNKGEFAQAEAAVNRSLRLSRDLEPRGPLVCQLVSIAMDGMTFSAIERL
                     VLTNPQLTAEQCDRLIALLAQHQRGLLNRTDEGLKLEYIMSRNAIEDLQSGRLTLQDL
                     LSMNGTESANPSTSTPSPAFNYDTEITALNRLYHLLLAEVNEPTAREATLGEFRQEMD
                     RLRADINRFKASPAPGQPADEDQRLINAPGFLTHFLIVAVEPFFDANRRVTAQLAGTQ
                     MLLALRRYEIAHGSLPVDLQTAAAESVLKAVPLDPYDGQPLRLATVGGQPTVYSVGKD
                     LKDDGGQADWKFGQQPGDYRFVLPPRG"
     gene            1714..3600
                     /locus_tag="VFV87_11685"
     CDS             1714..3600
                     /locus_tag="VFV87_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5104468.1"
                     /translation="MSVAVIVAGSSAKAAPTAEQRAEILALGTLMTKAGNFFKDGKFK
                     ESAEVVKEAQARLEKLGEGADEPMVGQLQPLYQRLIKAHALLELEGVALPELKPLEAK
                     PAAGKPAGDKPGAPAGARTGGISFVQQVAPILNTRCGNCHIRNARGMFSMSTYDALMK
                     GPPAGKVVIAGNVDGSDLIVKVKDREMPPNGAGIPDGELAVLTKWVEEGAKFDGADPA
                     VALPSLLPAGAGQPAAPMVKVEAATGKETVSFAMDIAPVLAKSCTGCHGTNRPRNNFS
                     LNTIESLLRGGDRGEPVLPGKPADSLLVKKLKGSADGAQMPMNQTPLDGAVIAKIEKW
                     IEEGAKFDGPDPKQPIAEVAAIAKANSQTHEELTADRAKLAGVNWNLGMPGITANRAE
                     STNFLVLGNVGENTLADIAQRAEALAPKVGDVFKAPPDQALIKGRMTVFVFGERYDYG
                     EFGKMVEKRDLPPMWRGHYRYSIIDAYGAVLTPRASDYSLDALIAQQLGAVYVASQGR
                     GVPHWFAEGSGRVVAERLGTAGDKRINAWEDELPRAISGMSAPDDFLTGKLPPEDADL
                     CAFSFVKFLMTDSSKYQAVLGALRKGDDFNAAFSAAYKGTPNQLAAAWVRKPPKPSKR
                     PAAK"
     gene            3713..>4458
                     /locus_tag="VFV87_11690"
     CDS             3713..>4458
                     /locus_tag="VFV87_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104469.1"
                     /translation="MCAPAKRRRSGGTLLELLAVIFILGLLMALLLPAIQAAREAGRR
                     IKCQNNLRQWGVGIWSYENTRGEFPPGYRMNTPTASFVPGVLPFVEQSTLPYDTNRDW
                     DDPANATAIATPLAILLCPSSPSGRRLDTSGGAPAAAGDYAPTHGVNAVYCDLAGWPH
                     YTPPNENGVLIFEPLLASAVTDGLSQTILLVEDAGRPRLWRRGTAAAGVAENGGWADP
                     NYEIALDGSDTLLTGPGQALGTCVMNCTNN"
BASE COUNT          843 a         1485 c         1399 g          731 t
ORIGIN      
        1 cgctgccccg cctgccatag gcgcagcgcc gggaccgcca cctgcgccac cgacatcacc
       61 ggccgccgga gcgggactgg tcgcagcagg ggaggtccct gcctgggccg gacagcgcag
      121 cgatgagccg ttcgacgtga aggcgtttct tgccagccgg gctgccccgc ccgacaacgc
      181 cgcaccgctc tacataactg ctctcgcccc actcagcggc gaattgggcg gcgcgtccgc
      241 cgtcccgctg gaacgggaaa ttggcgaatt ggcgaacatc gaccagctcg ccgccggcag
      301 cgtcactccg cagcagcttg accaggtgct ggccaaagcc gcgccagcga tccgactgat
      361 tgatgccgcc caagcgaagc cgaaatgcgt gtttataacc gggctgggca tcgacgccct
      421 gttgccccac gcccaagccg cccggaccgt aacgcgcctt tcggtgctac agctacagca
      481 ggcacgcaac aagggcgagt ttgcacaggc agaggctgcc gtgaaccgct cactgcggct
      541 gtcgcgcgat ttggagccaa ggggaccgct ggtttgccag ttggtgtcga tcgcaatgga
      601 cggcatgacg ttctcggcaa tcgagcggct cgtgctcacg aatccccaat tgactgccga
      661 gcaatgcgat cgcctcattg ccttactcgc ccagcatcag cgcgggctgc tgaatcgcac
      721 ggacgagggg ctgaagctcg agtacatcat gagtcgcaac gccatcgaag atttgcagtc
      781 aggtcggctg accctgcaag accttttgtc catgaacggt acagagtccg cgaatccctc
      841 cacttcaacc ccttcacccg ctttcaacta cgataccgag atcaccgctc tgaatcgcct
      901 gtaccacttg ctcctcgccg aagtgaacga gccaaccgcg cgcgaggcaa cactggggga
      961 gtttcgacag gaaatggatc gactgcgagc ggacatcaac aggttcaagg ccagtccggc
     1021 gccaggccag cccgcggacg aggatcagag actcatcaac gcgcccggat tcctgacaca
     1081 cttcctcatc gtagccgtcg aacccttctt cgacgccaat cggcgcgtca ctgcccagct
     1141 tgccggcacg caaatgcttc tcgccttacg gcgctatgaa atcgcgcacg gcagcctacc
     1201 ggtggatctg caaacagccg cggcggaatc ggtgctgaaa gcggttccgc ttgaccccta
     1261 tgacggccag ccgctgaggc ttgcgacggt tggcggccag cccacggtct attccgtcgg
     1321 caaagatctc aaggacgacg gcggtcaggc cgattggaag ttcggccaac aacccggtga
     1381 ctatcgcttc gtcctgccgc cgcggggcta aaggggcttg aggaactcga tcagagccga
     1441 tctgttggca ggcgataggt cgctctgccc gtcttgggaa gctattgcgc aggccggccc
     1501 ggcgcgcgtc gaacaggcgg ccgggtgtgc gccaatcgat ggtttcacca agttccattg
     1561 gccggcgcct ttgagtacct ttttccccgc tgggcgttta aactggaaga cttgccggct
     1621 ggggctgcgc gccggcgact cagccctaag ttcaacggaa gcagcgactt gcggagttac
     1681 cacatgcctg gcattcgtct tgcagttctc gttttgtcag tcgctgtgat cgttgccggt
     1741 tcctccgcga aggccgctcc cactgccgag cagcgggccg aaatcctggc cctgggcacg
     1801 ctgatgacca aggccggcaa cttctttaaa gacggcaaat tcaaggaatc ggccgaggtg
     1861 gtgaaggagg cgcaggcccg gctggagaag cttggagaag gagccgatga gccgatggtc
     1921 ggccagcttc agccgctcta tcagcggctg atcaaggccc atgctctgct ggagctcgaa
     1981 ggcgtcgcgc tccccgaact caagccgctg gaggccaagc cggccgccgg caagcccgcg
     2041 ggggataagc caggtgctcc ggcaggtgct cgcacggggg gcatcagctt tgtccagcag
     2101 gtcgcgccga ttttgaacac gcgctgcggc aactgtcaca tccgcaacgc ccgcggcatg
     2161 ttctcgatgt ccacctacga tgcgctgatg aagggaccgc cggccggcaa ggtggtcatt
     2221 gccggcaatg tggatggcag cgacctgatc gtcaaagtga aggaccgcga aatgccgccc
     2281 aatggcgcgg gaattcccga cggcgagttg gcggtcctca cgaaatgggt cgaagaaggc
     2341 gccaaattcg acggcgccga tcccgccgtc gcgctcccat cgctgctgcc cgccggcgcg
     2401 ggccaacccg ccgcgcccat ggtcaaagtc gaagcggcca ccggcaagga aaccgtcagc
     2461 ttcgcgatgg acattgctcc tgtccttgcg aaaagttgca ccggctgcca cggcaccaat
     2521 cgcccgcgga acaacttcag cctgaacacg atcgagagcc tcctccgcgg cggcgaccgc
     2581 ggcgagccgg tcctgcccgg taagccggct gacagcctgc tggtcaaaaa gctcaaaggc
     2641 agcgccgacg gcgcgcagat gccgatgaac cagacgccgc ttgatggcgc ggtcatcgcc
     2701 aaaatcgaga aatggatcga ggaaggggcg aaattcgacg gccccgatcc caagcagccg
     2761 atcgccgagg tcgctgcgat cgccaaggcg aatagtcaga cgcacgaaga gcttaccgcc
     2821 gaccgggcca agctggccgg agtgaactgg aacctgggga tgccgggcat tacggccaac
     2881 cgggccgaat cgacgaattt tctcgtgctc ggcaatgtgg gcgagaacac cctggccgat
     2941 atcgcccagc gggccgaggc gctggccccg aaagtcggcg acgtcttcaa agccccgccc
     3001 gatcaggcgc tcatcaaggg gcggatgacg gtcttcgtct tcggcgagcg ttacgactat
     3061 ggcgaattcg gcaagatggt cgagaaacgc gatctgccgc cgatgtggcg cggccactat
     3121 cgctatagca tcatcgacgc ctacggtgcc gtgctgacgc cgcgagccag cgactattcg
     3181 ctcgatgccc tgatcgccca gcagcttggt gcggtctatg tcgcctcgca gggacggggc
     3241 gtcccccact ggttcgccga gggctccggc cgcgtggtgg cggagcgact gggaaccgcg
     3301 ggcgacaaac gcatcaacgc gtgggaagac gaactcccgc gggccatttc cggcatgtcc
     3361 gctcccgacg actttctcac cggcaaactg ccgccggaag atgcggacct ctgcgcgttc
     3421 agcttcgtca agttcctgat gaccgacagc agcaagtacc aggcggttct cggcgcgctc
     3481 cgcaaagggg acgacttcaa cgccgccttt tccgccgcct acaaaggcac gcccaaccag
     3541 ctcgccgccg cctgggtccg caagccgccc aaaccaagca agcgcccggc cgcgaagtag
     3601 ccgatccaat cgaattcgta attctgccag gcgggcgaaa ggctgacatt cgcccgccaa
     3661 ctcgctaccc tgatgcttcg cagcggaacc acttcggcca ggggcactgg caatgtgcgc
     3721 accagcgaaa cgacggcgat ccggcggcac gctcctggag ctgctggccg tgatcttcat
     3781 tctgggcctg ctgatggctc tgctcttgcc ggcgattcag gcagcacgcg aagcgggcag
     3841 gcgcatcaag tgccagaaca acctgcggca gtggggcgtc ggtatctggt cgtacgaaaa
     3901 cacccgcggc gagttcccgc cgggctatcg catgaacacg cccaccgcgt cgtttgtgcc
     3961 gggtgtgctg ccgttcgtcg agcagtccac gctcccctac gacacgaaca gggattggga
     4021 cgatccggcc aacgccacgg cgatcgccac gcccctggcc atcctgctct gcccgtcgtc
     4081 gcccagcggc cggcggcttg atacgtccgg cggcgcgccg gctgcggcgg gcgattatgc
     4141 tccgacgcac ggcgtcaacg ccgtctattg cgatctcgcc ggctggccgc actatacgcc
     4201 gcccaatgag aacggcgttt tgattttcga gccgctgttg gcctccgcgg tgaccgacgg
     4261 cttgtcgcag acgatcctgc tggtcgaaga tgccggccgg ccgcggctct ggcggcgcgg
     4321 aacggccgcg gcgggcgtcg ccgagaatgg cggctgggcc gatccgaact acgaaatcgc
     4381 cctcgacggc tccgacacgc tgctgaccgg gcccgggcag gccctgggca cctgcgtcat
     4441 gaactgcacc aataacaa
//
LOCUS       DASUGB010000609         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_762984, whole genome shotgun sequence.
ACCESSION   DASUGB010000609 DASUGB010000000
VERSION     DASUGB010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762984"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFV87_11695"
     CDS             complement(<1..601)
                     /locus_tag="VFV87_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5104470.1"
                     /translation="MGLPKATLPFGPELMLQRVVRLLSGVVSPVVVVAAPDQALPPLG
                     DEVLLARDQREGRGPLEGLLAGLTAIAPHAQAAYATSCDVPLLVPAFVREMIRRLAQG
                     GPVVPTGQEPLIDANSLPAPQQERNNVGRNDIAVPVDGQFHHPLAAVYRTSVARFIAE
                     LLAADRLRPLFLFERAAICRVGVDELRAVDPRLDTLKNLN"
     gene            752..1570
                     /locus_tag="VFV87_11700"
     CDS             752..1570
                     /locus_tag="VFV87_11700"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HEX5104471.1"
                     /translation="MVLRLQLRNRSDVPLEVEGITADVLRDKSLADIEKLDIFQGNVK
                     LPLAEFFSVSGDPADEVHDWEGDLAGVHWIGAKMTAGRVVVHGNAGRHVGSEMAGGEI
                     HVLGDASDWLGGEMHGGLIHVRGKAGHLVGSAYRGSAKGMTKGTILVGGDAGNEIGHS
                     MRRGLVAIGGNIGDLAGLNMLAGSIFVFGTAGIRHGAGMKRGTLAFLGPESPPLLPTF
                     RRACRYRPEALLLVYRELTRHEFAVPDDLLTAACDLYNGDFLEGGRGELLVKAV"
     gene            complement(1613..>2672)
                     /locus_tag="VFV87_11705"
     CDS             complement(1613..>2672)
                     /locus_tag="VFV87_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5104472.1"
                     /translation="SSGPAAIDQDRIFARTLYELLVNSRLKVSLRPANASADSADQAA
                     AELAQSLSAYDCVVVIGDAGGVSISQVRSAAPLLIDARDHAFHPGTKQLTAYRANPRE
                     VSAVYTGVVPIVYKAQRMLLDSLIQSTFWSVITITPLLMWIARSASAGLVAMLPNVLP
                     ILMVFGGMGWLDIDVDVGSMMTASIALGVAVDDTIHYLNWFREELDRLGDRKQAILAA
                     YKHCATPTLQAATISGLGLSIFAISTFTPTQRFGTLMLVILWLGAVAELIFFPALLAG
                     PLGMVFKPRQKAGPEAATPQPAAAGPPQLQVIRHDDMAEDEAADEAEEAAVGGVPAPH
                     ARPSMPRPARRDVPHRRA"
BASE COUNT          462 a          861 c          909 g          440 t
ORIGIN      
        1 gattgaggtt tttcagcgta tccagccgcg gatcgacggc tcgcagctcg tccacgccaa
       61 cgcgacatat agccgcccgc tcgaaaagga agagcgggcg gagccgatcg gcggcgagca
      121 attcggcaat gaatctggcc acgctggtgc gataaacggc ggccaagggg tggtggaact
      181 ggccatcgac cggtacggcg atgtcgtttc gcccgacgtt gtttcgctcc tgctgcggcg
      241 cagggagaga attggcgtcg attagcggtt cttgtccggt ggggaccacc ggaccacctt
      301 gggcgagccg ccgaatcatc tcgcggacaa atgccggcac gagcagcggc acgtcgcagc
      361 tggtggcgta ggcggcctga gcgtgcgggg caatcgccgt caacccagcc agcagccctt
      421 ccagcggccc gcgtccttcg cgctggtcgc gggccagcag cacctcgtcc cccagcggcg
      481 gtagcgcttg gtcgggagcg gccaccacca caaccggcga caccacgcca gacaatagcc
      541 gcacgactcg ctgcagcatc agttccggcc cgaacggcag cgtagccttg ggcaggccca
      601 tgcgcgaact gcggccgcca cagagaacga tgccgcccac ttgagtttct gatttcggat
      661 ttcggctttc ggatttcgac atcagccggc aatcccgctg agtgtgatat acttccgctt
      721 caaatcctaa atcctaaatc cgacaacgga aatggttctc cgcctgcagc ttcgcaaccg
      781 ctctgacgtg cccctggagg tggaagggat caccgccgat gtcctgcgcg ataagtcgct
      841 ggccgacatc gagaagctcg acatatttca gggcaacgtc aagctgccgc tggccgagtt
      901 tttttcggtt agcggcgacc cggccgacga agtacacgac tgggaagggg acctggcggg
      961 cgtccactgg atcggcgcga agatgaccgc ggggcgggtc gtcgtgcacg gcaatgcggg
     1021 gcggcatgtc ggcagcgaga tggccggggg cgagattcac gtgctgggcg atgccagcga
     1081 ctggctgggg ggcgaaatgc acggcgggct gatccacgtc cgcggcaagg cggggcacct
     1141 tgtcggctcg gcctaccgcg gcagcgccaa gggcatgacc aagggaacca tcctggtcgg
     1201 cggcgacgcc ggcaacgaaa tcggccacag catgcgccgc ggcctggttg ccatcggcgg
     1261 caacatcggc gacctggccg gcctgaacat gctggccggc agtattttcg tttttggaac
     1321 ggccggcatt cgccacggcg cggggatgaa gcgcggcacg ctggccttcc tggggccgga
     1381 gtcgccgccg ctgttgccca ccttccgccg cgcctgccgc taccggcccg aggcgctgct
     1441 cctggtctat cgcgagctga cgcggcacga attcgccgtg cccgacgacc ttctcacggc
     1501 tgcctgcgac ctctacaacg gcgacttcct ggaggggggc cgcggcgaat tgctcgtcaa
     1561 agccgtctga atcccggcgt ctcaagccgc tatttcacgt tcttcggcgg gctcaggcgc
     1621 ggcggtgggg cacgtcgcgg cgcgcgggcc gcggcatgct cggccgggca tgcggggcgg
     1681 ggacgccgcc gaccgcggct tcttcggctt cgtccgcggc ttcgtcttcc gccatgtcgt
     1741 cgtggcggat cacttgcagc tgcggcggtc cggcagcggc aggctgcggc gtagcggcct
     1801 ctggacctgc cttttggcgc ggcttgaaca ccatgcccag cggcccggcc aatagtgccg
     1861 ggaagaagat cagctcggcg accgcaccca gccacagaat gaccagcatc aaggtgccaa
     1921 accgctgcgt gggcgtgaac gtgctgatgg cgaagatcga cagtcccaga ccgctgatgg
     1981 ttgcggcctg cagcgtcggc gtggcgcagt gcttataggc agcgaggatc gcctgcttac
     2041 ggtcccccag ccggtccagc tcttcgcgga accagttcag gtagtggatg gtgtcgtcca
     2101 ccgccacgcc caaggcgatg ctggcggtca tcatcgagcc gacatcgacg tcgatatcga
     2161 gccagcccat gccgccaaaa accatcagga tcgggagcac gttgggcagc atggccacca
     2221 ggccggcgct ggcgctgcgg gcgatccaca tgagcagcgg cgtgatcgtg atcaccgacc
     2281 agaacgtgct ctggatcagg ctgtcgagca gcatccgctg cgccttatag acgatcggca
     2341 cgacgccggt ataaacggcc gacacctcgc gcggattggc tcgatacgcg gtcagctgct
     2401 tggttccagg gtgaaacgcg tggtcgcggg cgtcgatcaa taggggagcg gcgctgcgca
     2461 cctgggagat gctgacgccc cccgcatcgc cgatcaccac cacgcagtcg taagccgaaa
     2521 gcgactgggc cagttctgcc gctgcctgat cggctgagtc cgcggaagcg ttggctggcc
     2581 ggagcgaaac cttgagccgc gagttgacga gcagctcata gagcgtgcgg gcaaagatcc
     2641 gatcctggtc gatggccgcg gggccgctgg ac
//
LOCUS       DASUGB010000610         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_346418, whole genome shotgun sequence.
ACCESSION   DASUGB010000610 DASUGB010000000
VERSION     DASUGB010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346418"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFV87_11710"
     CDS             <1..670
                     /locus_tag="VFV87_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5104473.1"
                     /translation="AVPSKSMLSVFQDGGPLMYPIALCSVVLLVVVFERFISLRKGRV
                     IPRPFVKRLLEQLREGQLDREKALKLCEDNRSPVAMVFAPAVKKWGRTSVEVEQAILD
                     SGERVTNQLRRYLRIMNGIAQVAPLMGLLGTVLGMISSFNGISTTAGADGQREMLAGG
                     IAQALITTAAGMVVAIPALISYMYFVGRVDQLVTEIDSLGQQVVELISADADSRSTKR
                     AKAA"
     gene            711..1130
                     /locus_tag="VFV87_11715"
     CDS             711..1130
                     /locus_tag="VFV87_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5104474.1"
                     /translation="MPLKTHQDDMPAINLTPMIDIVFNLIIFFMVGARFTELERKVDL
                     TVPQVADIKALTPTPDKRNINVYRDGRIELDSQSVSLEQLTAALASAHQEYSDLGVVV
                     RGDQQTPFQNVASVLTACRQAGITEMGISVRLGTKER"
     gene            1132..>2463
                     /locus_tag="VFV87_11720"
     CDS             1132..>2463
                     /locus_tag="VFV87_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104475.1"
                     /translation="MGLGAAIPLLRDLSDRTLAIILAVGMLGVFVILATLIWTRWGQA
                     RPVSKCIALSLLAHFLLVVYAYSTHILFGPPGKWTGQTVMVRLSDAADYQEAAPAKHS
                     DPEPWEQAGMEDVPLLEAPPLERLPTPTTEPQRAAVAVAPPKIPQPQPADIAIARPPT
                     PPLPVPAAPPLLPEKPANPTPPDQVADTPRESAPDVEATAQANSFDGVPDEVTSPRPD
                     TPRLTDLLPDNDASGDSTAAESASLPSNPLRDPGGAVATQPAEAGNPTELPAEPLPTP
                     APAANAGEQFTAAASGIPRRLGDGREVPEPLRARVAADRLQAAQPFGASPRTEAAVAA
                     ALQWLASVQSADGRWDADSLGAGRETRTLGHDRRGAGAQADTGISGLALLAFLGNGET
                     HLEGKHRETVQHGLEFLLASQASDGSLAGNSEFFAAMYSHGIATLALSEAYA"
BASE COUNT          400 a          911 c          744 g          408 t
ORIGIN      
        1 tgcggtgccg agcaagagca tgctctcggt ctttcaagac ggcggaccgc tgatgtatcc
       61 gatcgccctg tgctcggtcg tcctgctggt cgtcgtcttc gagcgcttca tctcgctccg
      121 caaggggcgc gtcatcccgc ggccgttcgt caagcggctc ctggagcagc tccgcgaagg
      181 ccagctcgac cgcgaaaagg ccctcaagct gtgcgaagac aatcgcagcc cggtcgcgat
      241 ggtcttcgcc cccgccgtca agaaatgggg ccgcacgtcg gtcgaagtgg aacaggcgat
      301 cctcgactcg ggcgagcgcg tcaccaatca gctccgccgc tatctgcgga ttatgaacgg
      361 catcgcccag gtcgcgccgc tgatggggct gctcgggacg gtgctcggga tgatcagctc
      421 gttcaacggc atctcgacca ccgccggtgc cgacggccag cgcgagatgc tcgccggcgg
      481 catcgcccag gccctcatta ccaccgcggc cggcatggtc gtcgccatcc ccgccctcat
      541 ttcctacatg tatttcgtcg gccgcgtcga tcagctcgtg accgaaatcg actcgctcgg
      601 ccagcaagtc gtcgagttga tctccgccga tgccgattcc cgctccacca aacgcgccaa
      661 agccgcgtaa tctctgtccc ctgtctcctg tcccctggct cctggcctcc atgcccctca
      721 agacccacca agacgacatg ccggcgatca acctgacgcc gatgatcgac atcgtcttca
      781 acctgatcat cttcttcatg gtgggggccc ggttcaccga attggagcgg aaggtcgatc
      841 tcacggtccc gcaagtggcc gatatcaagg ccctcacgcc gactcccgac aagcggaaca
      901 tcaacgtcta tcgcgacggc cgcatcgagc tcgacagcca gagcgtctcg ctggagcagc
      961 tcaccgccgc gctcgcctcg gcccatcagg agtacagcga tctcggcgtc gtcgtccgcg
     1021 gcgatcagca aacgccgttt cagaacgtcg cctccgtcct gaccgcctgc cggcaggccg
     1081 gcatcacgga gatgggcatt tcggtccgcc tgggcaccaa ggagcgttaa tgtgggcctg
     1141 ggcgccgcga ttcccctctt gcgcgacctc tccgaccgca cgctggcgat cattctggct
     1201 gtgggaatgc tcggcgtgtt cgtcatcctg gcgaccctca tctggacccg ctgggggcag
     1261 gcacgtccgg tttccaagtg catcgccctc tcgctgctcg cacattttct gctggtcgtc
     1321 tatgcctaca gcacgcacat cctcttcggc cctcccggaa aatggaccgg acagaccgtg
     1381 atggtgcggc tgagcgatgc ggctgactat caggaagccg cgccggcgaa gcacagcgac
     1441 ccagagccgt gggagcaggc cggcatggag gatgtgccgc tcctggaggc tccgccgctg
     1501 gagcgcttgc cgacgccgac caccgagcca cagcgggcag cggtcgccgt cgctccaccc
     1561 aaaattccgc agccgcagcc ggcagacatc gccatcgctc gtccgccgac gccgcccttg
     1621 ccggtgcccg ctgcaccgcc gctgttgccc gagaagcccg cgaatccgac gccgcccgac
     1681 caggtggccg acacgccgcg tgagtcggcg cctgatgttg aagcaaccgc gcaggcgaat
     1741 tcgttcgatg gtgttcctga cgaagtgacc tcgccgcggc ctgatactcc gcgattgacc
     1801 gacctcttgc ccgacaacga cgcctccggc gattccaccg ccgcggaatc cgcctcgctc
     1861 ccttccaacc cgctccgcga tccgggtggc gcggtagcaa cgcagccggc tgaagccggg
     1921 aatccaacgg agctgcctgc ggaaccgctt ccaacgcccg cccctgccgc gaacgcgggc
     1981 gagcagttca ccgcagccgc ttccggcatt ccgcggcgct tgggagacgg ccgcgaagtg
     2041 cccgagccct tgcgggcccg cgtcgctgcc gaccgcctgc aagccgcgca gccgttcggc
     2101 gcttcgcccc gcaccgaagc cgctgtcgcc gcggcactgc agtggctcgc ctccgtccaa
     2161 agcgccgatg gccgctggga tgccgactcc ctcggcgcgg gccgcgaaac gcggaccctc
     2221 ggccacgacc gccgcggcgc gggcgcccag gccgataccg gcatcagcgg cctggctctg
     2281 ctcgcattcc tcggcaacgg cgaaacgcac ctggaaggca agcaccgcga aacggtccag
     2341 cacggtttgg agtttctcct tgccagccag gccagcgatg gcagcctggc cggaaattcc
     2401 gaattcttcg ccgccatgta ctcgcacggc attgcgacgc tcgcgctcag cgaggcctat
     2461 gcc
//
LOCUS       DASUGB010000611         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_207905, whole genome shotgun sequence.
ACCESSION   DASUGB010000611 DASUGB010000000
VERSION     DASUGB010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207905"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VFV87_11725"
     CDS             <1..1261
                     /locus_tag="VFV87_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104476.1"
                     /translation="HETRNFWLLIVYQVVLRTGWIFKTESVVMPHAADALDHTGLARG
                     WLPLLNRLGQSIPPVLAARRIKNQRKKKRAFIGTTASMTLCFLGLTSLWLIPGLAQFR
                     FTAILFLFLYALFFAAIGINNLAYNTIQGKLIRPNWRGRLLTLADAIGATSAMICAAT
                     LLLMWLHDDGADYAAIFGFTTVLFAAASVMSWFLKEQPDNHYEPARGVAHVFRAAWRT
                     LAEDANFRRLAIVAALFSSTLVLFPHYQAIARDELQLGTRWLVWWVVAQNAGTLLFSV
                     LTGPLADAFGNRLALRMLTLLIVAGPLAAIAAIYWPEAGRVAFPLVYLFIGMTPVAQK
                     TFNNYTLEISEPENHPRYLSTLSLSMAAPLLASPLVSLALIYVRFEVVYLVVIVLLVL
                     GWLMTFGLTEPRAGGRPVVLSEDSITE"
     gene            complement(1251..2828)
                     /locus_tag="VFV87_11730"
     CDS             complement(1251..2828)
                     /locus_tag="VFV87_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5104477.1"
                     /translation="MSDEIQDASSASAGAPATAEAGDLVGAVRRGTRWVFYAQIASQL
                     VSLGVLAVMYRLVKPEEYGLFAAALPAVMLPRMAATLGLSTAVLQEPELSHEQKSGLF
                     WLSVVLGLAAAAVTAVCGPLLAAAYNQPPLAEICLALAGATIIAVLGNQHLALLEREL
                     KLREASAARLVALVSGGLAGVLTARRGAGVWALVAQQYAELAVLTVWVWMLEPWRPGW
                     PGPAARLRQFVAFSTSYSLSQLVNYAALNLDKIVIPLALGAAGERVLGLYSQAQNLMM
                     RPVSVLTSPLTGVMVAGLAKARDDQALRTELAAKFFRLAGVGLLPCAVGLAVVAPDVM
                     LVLGGQRWSEAGMLLAVLAPAIVAHGLNNLAAYVLSSAGRAGYLLLAMLLLLLLLVQG
                     MLAGLFFGRTFLAGSTADAALAPALGMAIAQTAAVTLVWLGPYLWFCLRTAQLDDATV
                     LRVLLPPLRAALLMGAVVWLLRLMLIQTPSISPAVRLLALIGAGVLSYALLAQRDVRW
                     AIGELTVERGATPAATP"
     gene            complement(2883..3485)
                     /locus_tag="VFV87_11735"
     CDS             complement(2883..3485)
                     /locus_tag="VFV87_11735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5104478.1"
                     /translation="MPGIDWDVHYQSGTPPWETGQPSAELARVIAEEKIQPGRVIELG
                     CGSGINAVWLAQQGFDVTGVDFNRLAIEKARQRAIEAEVTIRFLLADVTDWKDDAEPF
                     PFFFDRGCYHSVRDLNLPGYLRTIERVTSPGSLGLILTGNAREPSPPGQGPPVVSEEQ
                     LRAELPPLLEIVRLREFRFDVADSAGPSPLAWSCLVRRSA"
     gene            complement(3527..>3844)
                     /locus_tag="VFV87_11740"
     CDS             complement(3527..>3844)
                     /locus_tag="VFV87_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrrolo-quinoline quinone"
                     /protein_id="HEX5104479.1"
                     /translation="RAVKLDTGERLWETFQPTTGGDRRASHGTAFLTKNGDRYFLFSE
                     TGDLVIARLSPEKYEEISRANLLEPTAECFGRSVVWSHPAYANKCVFARNDKEIVCAS
                     LAK"
BASE COUNT          668 a         1387 c         1174 g          615 t
ORIGIN      
        1 ccacgagacc cgcaacttct ggctcctgat cgtctatcag gtcgtcctcc gcaccggctg
       61 gatcttcaag accgaaagcg ttgtgatgcc gcacgccgcc gacgccctcg atcacacggg
      121 gctggctcgc ggctggttgc cgcttctcaa tcgcctgggc cagagcattc cgcccgtgct
      181 ggctgcgcgg cgaatcaaaa accagcggaa aaagaagcgg gccttcatcg gcacaaccgc
      241 ctcgatgacg ctctgcttcc tcggcctgac cagcctgtgg ctgatcccgg gcctggctca
      301 attccgcttc acggcgatct tgtttctttt cctgtacgcc ctgttctttg cagcgatcgg
      361 catcaacaac ctggcctaca acacgattca gggaaagctg atccgcccca actggcgggg
      421 gcggctcttg acgctggccg atgccatcgg ggccacgtcg gcgatgatct gcgccgccac
      481 gctcctcctc atgtggctgc acgacgacgg cgccgattac gccgcgatct tcggcttcac
      541 gacagtcctg ttcgccgcag cctcggtcat gtcgtggttc ctcaaggagc agccggacaa
      601 ccactacgaa cccgcgcggg gcgtggcaca cgtgttccgt gcggcctggc ggacgctcgc
      661 cgaagacgcc aacttccgcc gcttggcgat cgtcgcggcc ctcttcagct cgacgctcgt
      721 cctgttcccg cattatcagg cgatcgcccg cgacgagctg cagctcggca cccggtggct
      781 ggtgtggtgg gtcgtggccc agaacgccgg cacgctcctg ttcagcgtgc tgaccggccc
      841 gctggccgac gctttcggca accgcctcgc gctgcgaatg ctcacgctcc tgatcgtcgc
      901 cggaccgctg gcggctatcg cggcgatcta ctggcccgaa gccggccgcg tggcctttcc
      961 gctggtatac ttgttcatcg gcatgacgcc cgtcgcccag aagaccttca acaactacac
     1021 gctcgaaatc tccgagccgg aaaatcatcc gcgctatctc agcacgctca gcctgagcat
     1081 ggccgctccg ctgctcgcct cgccgctggt cagcctggcg ctgatttacg tccgcttcga
     1141 ggtcgtctat ctggtggtga tcgtcctgct cgtcctcggc tggctgatga cgttcggcct
     1201 caccgaaccc cgcgccggcg gccgccccgt cgtgctttcc gaggattcga tcacggagta
     1261 gccgccggcg tcgccccacg ctcgacggtc aactcgccaa tcgcccaccg cacgtcgcgt
     1321 tgggcaagca gcgcgtagct gagcacgcct gcgccgatga gcgccagcag cctgactgcc
     1381 ggcgagatgc tcggtgtctg gatcaacatc aaccgcaaca gccagacaac ggcccccatc
     1441 aagagcgccg ctcgcagcgg cggcaagagc acccgcaaca cggtggcgtc gtcgagctgc
     1501 gccgtccgca aacagaacca caaatacggc ccgagccaga cgagcgtgac cgcggcggtc
     1561 tgggcgatgg ccatgcccag cgccggcgcg agggccgcat cggctgtgct tcccgccaga
     1621 aatgtccggc cgaaaaatag ccccgccagc attccctgca ccagcaatag cagcaacagc
     1681 agcatcgcca gcagcaggta ccctgcccgg ccggctgacg acagcacata agcggccaga
     1741 ttgttgagtc cgtgagcgac gatcgccggc gcgaggacgg ccagtagcat gcccgcctcg
     1801 ctccaccgct ggccgccgag gacgagcatg acatccggtg caacgaccgc cagccccacc
     1861 gcgcatggca acagtcccac acccgccagg cggaagaatt tggccgccag ctcggtgcgc
     1921 agcgcctgat cgtcccgcgc cttcgccaga cccgcgacca tcacgcccgt cagcggcgag
     1981 gtgagcacgc tcaccggccg catcatcagg ttctgggcct gcgaatagag tcccagcacc
     2041 cgctcgcccg ccgcgccgag cgccagtggg atcacaatct tatcgaggtt caaagcggcg
     2101 tagttgacga gctggctcag cgaatacgac gtgctgaacg ccacaaactg ccgcaacctt
     2161 gccgccggac ccggccagcc gggccgccac ggctctagca tccagaccca gacggtgagg
     2221 acggccagct cggcatattg ctgggcgacc agggcccaca cacccgcccc gcggcgagct
     2281 gtcagaacgc cggccagccc gcccgagact agcgccacga ggcgcgctgc ggatgcctcg
     2341 cggagtttca gctcacgctc cagcagcgct aaatgctgat tgcccagcac ggcgatgatc
     2401 gtcgcaccgg ccagcgccag gcagatctcc gccaacggcg gctggttgta ggccgcggcc
     2461 aacagcgggc cgcaaacggc cgtcaccgcc gcagccgcca gcccgagcac cacagagagc
     2521 cagaacaggc cgctcttctg ctcgtgcgag agctcgggct cctgcaagac cgcggtcgaa
     2581 aggccaagcg tcgccgccat ccgcggcagc atcaccgccg gcagcgccgc ggcaaagagg
     2641 ccatactctt ccggcttcac cagccgatac atcaccgcga gcacgcccag cgagacgagc
     2701 tgcgaggcga tctgggcata gaagacccac cgcgtcccgc ggcggacggc gccgaccaaa
     2761 tcgccggctt ccgctgtagc gggcgcaccc gcactggcgc tggaagcatc ctggatttca
     2821 tccgacattc ggccatcgta gtcggcacgc tgcgcgggcg gaatggcgaa agactattcg
     2881 ccttacgcgc tgcgccgcac caggcacgac caggcgagcg ggcttggacc ggcgctgtct
     2941 gccacgtcga agcggaattc gcggaggcgg acgatctcca ggagcggcgg cagctcggcg
     3001 cgaagctgct cttcggagac gaccggcggg ccttggccgg gcggcgaggg ttcgcgagcg
     3061 ttgccggtga ggatcagccc gagcgacccg ggcgaggtca cccgctcgat cgtccgcagg
     3121 tagcccggca ggttcaagtc gcgcaccgag tggtagcagc cgcggtcgaa gaaaaacggg
     3181 aagggttcgg cgtcgtcctt ccagtcggtc acatcggcca gcaggaagcg gatggtcact
     3241 tccgcctcaa tggcccgctg gcgggccttc tcgatcgcca gccggttgaa atcgacgccc
     3301 gtcacgtcga atccttgctg cgcgagccag accgcattga tcccgctgcc gcagcccagc
     3361 tcgatgaccc gtcccggctg aatcttctcc tccgcgatca cccgcgccaa ttccgctgac
     3421 ggctgccccg tctcccacgg aggcgtccca ctctggtaat gcacgtccca atcgatcccg
     3481 ggcatggcaa gttccttaga agcacgcgac tcaacgcagt ttgcggttac ttcgccaaac
     3541 tcgcacacac gatctctttg tcgttccttg cgaagacgca cttgttcgcg taggcggggt
     3601 ggctccagac gacgctgcgg ccgaaacatt cggccgtggg ctccaggagg ttcgcccggc
     3661 tgatttcttc gtacttttca ggcgagagtc gggcgatgac cagatcgccc gtttcgctga
     3721 acaggaaata gcggtcgccg ttcttcgtca ggaacgccgt gccgtgcgag gcccgccggt
     3781 cgccgccggt cgtcggctgg aaggtctccc acagccgctc gccggtatcc agcttcaccg
     3841 cccg
//
LOCUS       DASUGB010000612         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1179903, whole genome shotgun sequence.
ACCESSION   DASUGB010000612 DASUGB010000000
VERSION     DASUGB010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179903"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VFV87_11745"
     CDS             complement(<1..1459)
                     /locus_tag="VFV87_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104480.1"
                     /translation="MDFVNKAYAQAAELFRSLSPGTRIVAGLLLAAIVVSLVYLFQFQ
                     ITGGDEFLLGGRPFTASEMTAIEAAFAKAGLGESTIVGNRIRIPRGKKEVYLAALADH
                     GALPADFYKYLDEATAADSPFASSRSLEIRRWNAKQKELALIISRMRGIESATVQYDE
                     EIKQGLTRQKRKTAMVALQTAGGGLEEEQLKAIRNVVASAYAGLDRQDITVTDMTSGL
                     SYGGAPGPGGVPEGENVYAMHKQQQERFYQRKITDQLSMIKGVVVGVNVELSPELEHR
                     LRTVKLDPKPVTVNSRETSKESTTQAPGPGGRPGAVPNGVGNQAVAVSTSTSNGQSST
                     TETQSDIVNVPGYEETVTDKVGLIPTIVTASIEVPASYFQQVWLQRNPVPPGEDPKKA
                     DAAELAQIETETVNRIKETVRNLLPPVAQGTNPYPHIVVSTYTDVPGPAAEAPGILVT
                     TQTWLAENWRMLAMAAIGLFSLAMLRSMIRSPAAPP"
     gene            complement(1527..1874)
                     /gene="fliE"
                     /locus_tag="VFV87_11750"
     CDS             complement(1527..1874)
                     /gene="fliE"
                     /locus_tag="VFV87_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX5104481.1"
                     /translation="MTPISSIPLVPSPTPLAPIQSPGAAFGAQAAGDAAQPASGNTTQ
                     PFQNILLEALDQVNSMQHEANQAVQQLVTGGDANPAEVLTTLQKADMSFKLMLQIRNK
                     LVQAYQEVNNIRI"
     gene            complement(1928..2353)
                     /gene="flgC"
                     /locus_tag="VFV87_11755"
     CDS             complement(1928..2353)
                     /gene="flgC"
                     /locus_tag="VFV87_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX5104482.1"
                     /translation="MFPALDISTSGLVAQRVRLDAISGNIANLSSMARDANGEMQPYQ
                     ARYVLFQTDESLQTRDGAAGVKVSSIETEQVEPLYRWQPEHPFAIKDGPKKGYVAYPQ
                     VNMTAEFVDALVATRAYEANLGVMELSKNMAQQSLRILG"
     gene            complement(2374..2784)
                     /gene="flgB"
                     /locus_tag="VFV87_11760"
     CDS             complement(2374..2784)
                     /gene="flgB"
                     /locus_tag="VFV87_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX5104483.1"
                     /translation="MLPQLFASTNIPILGEVLNFAQARHTVLAGNVANVNTPGYRLRD
                     LNESEFQARLREAIEQTEAARPSSPGLVRSETGDPLREVRRSLENILYHDDTNIDLEK
                     QITEMNKNQILHSFALTVMTDQFRLLEAAISERV"
     gene            complement(3065..>4227)
                     /locus_tag="VFV87_11765"
     CDS             complement(3065..>4227)
                     /locus_tag="VFV87_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5104484.1"
                     /translation="VMVTAFASIGSAVEAMRYGAFDYIEKPFDVEQLEELVARALRHG
                     MKVGRRSSVPQATDSWEGIMVGASREMQLLRQRITQAAPTDETVLISGESGTGKELVA
                     RCLHAASRRHGRALVSLNCPALSPQLMESELFGHERGAFTSADAPRVGRFELAEGGTI
                     LLDEISEIDLQLQAKLLRVLQQRTYERVGSSETRKADVRVIATTNRDLAAEVAAGRFR
                     QDLYYRLAVVPIEVPPLRGRREDVPLLARHFAAATAARLGNRVCDLSADALELLCSYH
                     WPGNVRELENLLTRAAVLSAGRSITAGDLRSWLMEPAVGDHPGSPSSAGAIEVGTKLD
                     EMERRLIEATLEHFGGHRAKAAHALGIGIRTLTNKLRLYGYAARAKSFSGRM"
BASE COUNT          699 a         1364 c         1325 g          839 t
ORIGIN      
        1 gcggcggtgc ggccggcgag cggatcatgc tgcggagcat cgccaggctg aagagcccga
       61 tcgcggccat cgccagcatc cgccagtttt cggccagcca ggtttgcgtc gtcaccagga
      121 tgcctggcgc ttcggccgcc ggcccgggaa cgtcggtgta cgtcgaaacg acaatgtgcg
      181 gataaggatt cgtcccctga gccaccggcg gcagtagatt gcgaaccgtt tccttgattc
      241 ggttgaccgt ctccgtctcg atctgtgcca gctccgccgc gtccgccttc ttggggtcct
      301 cgcccggcgg tactggattc cgctgcagcc agacctgctg gaagtagctc gcggggactt
      361 cgatcgaggc ggtgacgatc gtgggaatca ggccgacctt gtcggtgaca gtctcctcgt
      421 agcccggtac gttgacgatg tcggactgcg tttcggtcgt gctggattgt ccgttgctgg
      481 tgctggtcga gacggccacc gcctggttgc ccacgccgtt gggcactgcc cctggccggc
      541 cgcccggccc cggcgcttgc gtggtcgatt ccttggaagt ttcgcgggag ttgaccgtca
      601 cgggcttcgg atcgagcttc accgtgcgga gcctgtgctc cagctccggg ctcagctcga
      661 cattcacgcc cacgaccacg cctttgatca tcgagagctg gtcggtgatc ttacgttgat
      721 agaagcgctc ctgctgctgc ttgtgcatgg cgtacacgtt ctcgccctcg ggcactccac
      781 ccggccccgg cgccccgcca taggacagcc cgctcgtcat atcggtgacc gtgatgtcct
      841 ggcggtcgag cccggcatat gccgaagcga ccacgttgcg aatcgccttg agctgctctt
      901 cttccaaccc gcctccggcg gtctgcagcg ccaccattgc cgtcttgcgt ttctgacggg
      961 tcaggccctg cttgatttcc tcgtcgtact gcacggtggc cgattcgatc ccccgcatcc
     1021 gcgagatgat cagggccagc tccttctgct tcgcgttcca gcggcggatt tcgagcgatc
     1081 tgctggaagc aaacgggctg tcggcagccg tggcttcgtc gagatacttg taaaagtccg
     1141 ccggcagagc tccgtgatcg gcgagagccg ccagatagac ttcttttttc ccgcgcggga
     1201 tgcgaatgcg attgcctacg atcgtgcttt cgcccagccc ggcctttgca aaggccgcct
     1261 cgatcgctgt catttcgctg gcggtgaagg gccggccgcc gagcagaaac tcatcgccgc
     1321 cggtaatctg aaactgaaac agatacacga ggctgacgac gattgccgcc agcagcaacc
     1381 ccgcgacgat tcgcgtgccg ggcgaaagcg agcggaacaa ctccgccgcc tgtgcgtatg
     1441 ctttattgac gaagtccatc cgtggatctc caccatagcc gcccgcgcga agcgcgggag
     1501 tcgcctcgtg cgatcaagcg cggctgctat atccgtatgt tgttgacttc ctgatacgcc
     1561 tggaccagtt tgttgcgaat ctggagcatc agcttgaacg acatgtcggc cttctgcagg
     1621 gtcgtgagta cttcagccgg gttcgcgtcg ccgccggtga cgagttgctg cacggcctga
     1681 ttggcttcgt gctgcatcga gtttacctgg tcgagcgcct ccagcaggat gttctggaac
     1741 ggctgcgtag tgttgccgga ggcgggctgc gcggcatcgc cggcggcctg cgcaccgaat
     1801 gctgcgccgg gcgactgaat gggcgcgagc ggcgtgggcg atggcaccag cgggatgctc
     1861 gaaatcgggg tcatgcgatt ggtcctccgt caacacacac ggcggacggg ttgggaaacc
     1921 cgtcctacta gcccagaatt cgcagcgatt gctgcgccat gtttttactc agctccatca
     1981 cgcctagatt ggcctcatag gcgcgcgtag ccacgagcgc atcgacaaac tcagccgtca
     2041 tgttgacctg cggatacgcc acgtagccct tcttcggccc gtccttgatc gcgaagggat
     2101 gctccggctg ccagcggtaa agcggctcga cctgctccgt ctcgatgctg gaaaccttca
     2161 cgccggccgc tccgtcgcgc gtctgcagcg attcgtcggt ctgaaacagg acgtaccgcg
     2221 cctgatacgg ctgcatctcg ccgttggcgt cgcgggccat ggacgagagg ttggcgatgt
     2281 tgccggaaat cgcatccagg cgcacgcgct gggcgaccag gccgctggtg ctgatatcga
     2341 gggctggaaa catggacgtt cctttcagcg tgactagacg cgctcgctga tggccgcttc
     2401 gagcagccgg aattgatcgg tcatcacggt caacgcgaag ctgtgcagga tctgattctt
     2461 attcatctcc gtgatctgct tctccaggtc gatgttcgtg tcgtcgtgat agaggatgtt
     2521 ctcgagcgaa cggcggactt cccgcagcgg atcgccggtc tcactgcgaa ccaacccggg
     2581 cgacgaagga cgcgctgcct cagtctgttc aatcgcctcc cgcaaccggg cctggaactc
     2641 gctttcgttc aggtcgcgca gccgatagcc gggcgtattg acgtttgcga cgtttcccgc
     2701 cagcaccgtg tgccgagcct gggcgaagtt gagcacctcg ccgaggatcg ggatgttcgt
     2761 ggaagcgaat agctgcggca acatcacttg gctccgcgtg ggaggcgagc atcggccatt
     2821 gcaaccgttg tgccgctagc tctgcggaac gcggcggcag gctggtctcg attgcatgcg
     2881 aaggacgcaa atcggcgagg cggcgagctt tgcgtcaaaa ggaaaaactg ggcaagactt
     2941 cggccggcct catcttcggg cgaatgcatc tgcagccggc cggcaattcg tgccgaatcg
     3001 catcggcagg atttgccggt tggtgctgcc cgaaatggat tgtggcgagg catagaccgt
     3061 ccccttacat gcgtccgctg aacgacttgg cccgtgcggc gtagccatac aaacgcagct
     3121 tgttcgtgag cgtgcggatg ccgatgccca gcgcatgggc cgctttggcc cggtgcccgc
     3181 cgaaatgctc cagcgtggcc tcaatcagcc gccgctccat ctcatcgagc ttggtgccga
     3241 cttcgatcgc gccggctgaa ctcggcgaac cgggatggtc accgacggct ggctccatca
     3301 gccaggagcg caggtcgccc gccgtgatgc tgcggcccgc tgagaggacc gccgctcgcg
     3361 tcagcaaatt ctccagctca cgcacgttcc ccggccagtg gtacgagcaa agcaattcca
     3421 aggcgtccgc cgagaggtcg caaactcggt ttcccagacg cgccgcagtc gcggcggcaa
     3481 aatgccgggc caatagcggc acgtcctcac gccgaccacg gagcggcggc acttcgatcg
     3541 gcaccaccgc cagccggtag tacaaatcct gccgaaatcg tccggcggca acttccgccg
     3601 ccaggtcgcg gtttgtcgtg gcaatgacgc ggacgtcggc cttgcgcgtt tcgctcgatc
     3661 caacgcgttc ataggttcgc tgctggagga cgcgcagcag tttggcctga agctgtaggt
     3721 cgatctcgct gatttcgtcg agcagaatcg tgcccccctc ggccaactca aaccgtccga
     3781 ctcgcggtgc atcggcgctg gtgaaggcgc cgcgctcgtg gccaaaaagt tcgctctcca
     3841 tgagctgcgg ggaaagggcc gggcagttca ggctgaccag cgccctcccg tggcggcggc
     3901 tggcggcgtg caggcagcgg gccaccaact ctttgccggt cccgctttcg ccgctaatca
     3961 gcaccgtttc gtcggtcggc gcggcctggg taatccgttg ccgcaagagt tgcatctccc
     4021 gacttgcgcc taccatgatc ccttcccagg aatccgtcgc ttgcggcacg ctcgaccgcc
     4081 gcccgacctt catcccgtgc cgcagcgctc gggcaaccag ctcttcaagc tgctccacgt
     4141 cgaacggctt ctcgatgtag tcgaacgcgc catagcgcat tgcctcgaca gcagagccga
     4201 tcgaggcaaa cgccgtgacc atcacga
//
LOCUS       DASUGB010000613         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1597819, whole genome shotgun sequence.
ACCESSION   DASUGB010000613 DASUGB010000000
VERSION     DASUGB010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597819"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1122
                     /locus_tag="VFV87_11770"
     CDS             70..1122
                     /locus_tag="VFV87_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104485.1"
                     /translation="MPRFSVSASCALLTLAALIVPGARGQGPPVPSAADAPPVAEVAP
                     AAPAPAAPVITDAAPPAVRRALIVCGLPGDAEHRELFAESVESLVTGLTTHHGFALEN
                     VALYWGDEIAEGDGPALKSSRGIATRESLAEGAESLQASLRANDTLWVFVFGHSHYDG
                     RHAWLNIAGPDLNNQEFGRFFEGLKCREQVFFITTSASGFYLKPLAAAGRVVIVATEP
                     DLEVNETLFPHTLVKALADPPPYLEFDADRDGKLTLLDAYLWSARDIAQQYVTGELLA
                     TEHSLLDDTGDGRGTELQINYLSEELGGRLKAGQDWPAPPTGDGALARQIRLAHPPAP
                     PVPEVQPDPIIPAPSN"
     gene            complement(1262..>2592)
                     /locus_tag="VFV87_11775"
     CDS             complement(1262..>2592)
                     /locus_tag="VFV87_11775"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5104486.1"
                     /translation="YLLFDFSTVFPYLAASAVVPVEVLILGGQMPDHDHVAFHWWLNK
                     VLATGIFLLALVPLIFGGKIFNSLKVVMSTKLVLVFGFLLVLGIFFSRPAAWVEIWTG
                     FFKVGNVPALARDAEGNRAAEQPKVPQVENIFVELTQRGRLPLLDFSLIASIAALAAI
                     AGNGGLSNTPVSNYARDQGWGMGDKVGAIPSMIGGRGIGLTHEGCVFEPNEKTLPRWR
                     RWYWFLVRDQLLIWLPACLIGIALPSLLSVEFLPRGTGTDQWNSAVMTAGGVRDHVTS
                     PPLGTLVHATGLTSILAGPRWGNIFWALTLLCGFLVLGTTLVSTIDGIIRRWVDVFWI
                     SSRRLREVDPQNIRYVYFGVLIGYSLFGIVMLWFFNPKQLIEWATLGYNFALGFSCWH
                     TLALNRVLLPKPLRPGLVPTLSLTLGGIFFWLLGVVTLVEKLRSVGVIKL"
BASE COUNT          501 a          871 c          801 g          419 t
ORIGIN      
        1 gatcggcacg ctgctgccgc cggaaattgt cgcagaattg aagaccccgc cggagaaacc
       61 ataatggcga tgcctcgatt ctcagttagc gcaagctgcg cgctcctgac actagccgct
      121 ctgatcgttc ccggtgctcg cggccagggg ccgcccgtcc ccagtgccgc cgatgcgccg
      181 ccggtcgcgg aagttgcgcc cgccgcgccg gcgcccgcag cgcccgtaat caccgacgcc
      241 gcgccgccag ccgtccgccg ggcgctcatc gtctgcggat tgccaggcga tgccgagcac
      301 cgcgagctgt tcgccgagtc ggtcgaatcg ctcgtaacgg ggctgacgac ccatcacggc
      361 tttgccctgg aaaacgtggc cttgtactgg ggtgatgaaa tcgccgaggg cgacggccct
      421 gcgctcaagt ccagccgcgg cattgccacc cgcgagtcgc tggccgaagg cgctgagtcg
      481 ctccaagctt cgctcagagc aaacgatacg ctgtgggtgt tcgttttcgg ccattcgcat
      541 tacgacgggc gccacgcgtg gctcaatatc gccggaccgg acctgaacaa tcaggagttc
      601 ggccgcttct tcgaagggct aaagtgccgc gagcaggtct ttttcatcac cacctccgcc
      661 agcgggttct atctcaagcc gctggctgcc gcggggcgcg tggttatcgt cgcgaccgag
      721 cctgacttgg aagtgaacga gacccttttt cctcacacac tcgtcaaggc tctggccgat
      781 ccgccaccgt acctggagtt cgatgccgac cgcgacggca agttgacgct actcgatgca
      841 tacctctggt cagctcgcga catcgcccag cagtacgtca ccggcgaatt gctggcgacc
      901 gagcactcgc tcctggacga cacgggcgac ggccgcggga ccgagctgca aatcaactac
      961 ctctccgaag agcttggcgg ccgcctgaag gccggccagg actggcccgc cccccccacc
     1021 ggcgatggcg ccctcgcccg ccaaatccgc ctcgcccatc cccctgcccc gccggtgccg
     1081 gaagtccagc ccgatcccat cattcccgct ccctcaaact agtccgccga ggcccgcggc
     1141 cccacgtagg gtcagggagt gacgcgctcc aatcccaatc aagctctgtg tggcacacat
     1201 acgtgagttg atcgcacacg cattttcgcc tcacaactga ttggcgcagt gtcatcatcg
     1261 ttcagagttt tatcaccccc accgaccgca gcttttccac cagcgtgacg acgccgagga
     1321 gccagaagaa gatgccgccg agggtgaggc tgagcgtggg gacgaggccg gggcggaggg
     1381 gcttggggag gagcacgcgg ttgagggcta gtgtgtgcca gcagctaaag ccgagggcga
     1441 agttgtagcc gagcgtggcc cactcgatga gttgcttggg gttaaaaaac cagagcatca
     1501 cgatgccgaa cagtgaatag ccgatcagca cgccgaaata gacgtagcgg atgttctgcg
     1561 gatcgacctc gcggaggcgg cggctgctga tccagaagac atcgacccag cgacggatga
     1621 tgccgtcgat cgtgctgacg agcgtcgtcc ccaggaccag aaagccgcac agcagcgtca
     1681 gggcccagaa gatgttgccc caccgcgggc cggcgagaat gctcgtcagg ccggtcgcgt
     1741 ggacgagcgt gcccagcggc gggctggtca cgtggtcgcg gacgcctccg gcggtcatca
     1801 ccgccgagtt ccattggtcc gtgccggtgc cgcgcggcag gaactcgact gagagcaggc
     1861 tcggcagggc gatgccgatc agacaggccg gcagccagat cagtagctgg tctcgaacga
     1921 ggaaccagta ccaccgccgc cagcggggaa gcgtcttctc gttcggctcg aagacgcatc
     1981 cctcgtgcgt cagaccgata ccacgtcctc cgatcatgct gggaatcgcc ccgaccttat
     2041 cgcccatccc ccagccctgg tcgcgggcat agttgctgac cggcgtgttc gacaagcccc
     2101 cgttgccagc aatcgccgcc agggccgcga tcgaggcaat gagcgagaaa tccaggagcg
     2161 gcagcctccc ccgctgcgtc agctcgacga aaatgttttc cacctgcgga accttcggct
     2221 gctcggcggc tcggttgccc tccgcatcgc gggccagcgc cgggacgttc ccgaccttga
     2281 aaaatccggt ccagatctcc acccaagccg cgggccgcga gaagaaaata ccgagcacga
     2341 gcagaaagcc gaagaccagc acgagcttcg tgctcatcac gaccttgagc gagttgaaaa
     2401 tcttgccgcc aaagatgagc ggcacgagcg ccagtaggaa tatcccggtc gcaagcactt
     2461 tgttcagcca ccagtgaaag gcgacgtggt cgtggtcggg catttggccc cccaggatca
     2521 gcacttcgac cggcaccacc gcgctggccg ccaggtaggg aaacacggtg ctgaaatcga
     2581 acagcagata aa
//
LOCUS       DASUGB010000614         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2014252, whole genome shotgun sequence.
ACCESSION   DASUGB010000614 DASUGB010000000
VERSION     DASUGB010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014252"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1127
                     /locus_tag="VFV87_11780"
     CDS             144..1127
                     /locus_tag="VFV87_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104487.1"
                     /translation="MARMLQALKNLEARSPKPAPVAGVLSHLDATRPPAEPAGAQLAH
                     SRVAYEAAAPAVSVVPTMVMVESAWQALKAPYEPMVVETPPPIAPAAPPAAAEPPRVE
                     PPTAEARPVVPATRPPTGAERAVRRTLGEPHRRQPLADLASRLRQDAHQTGSRTLLLV
                     GIGAESTTHETALHVAALMAEEGGRVLLIDADLARRQLSTDLDSLQEPGFSDLARENV
                     SEQPTAQPTAFEGLAFLPAGRQRFIDLENSRADRLARALGQLSAEYDLVLLSGGQAND
                     PAAGPLGRLSDATYIVVELGTVEAGQAQQALRELRAGGARVLGCIATGVAA"
     gene            1140..1892
                     /locus_tag="VFV87_11785"
     CDS             1140..1892
                     /locus_tag="VFV87_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX5104488.1"
                     /translation="MPTDASAANAPADERRILISIATYNEIENLPAIIEKIWQVAPRA
                     DIVVIDDDSPDGTGRWCDEKASGEPRLRCVHRPSKLGLGSAILAAMQYATEHDYDYLL
                     NMDADFSHHPRYIPALLEVMHSADVAIGSRYCHGGTTKDWPATRKLMSRAVNAYARWL
                     LWLQPKDTSGGFRCYRVALLQSLAPQAVLSRGYSFQMEVLWRLKRLGARLAETPIEFA
                     DRERGQSKISKQEALSALWIIFRLGITHWIGL"
     gene            2013..2546
                     /locus_tag="VFV87_11790"
     CDS             2013..2546
                     /locus_tag="VFV87_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5104489.1"
                     /translation="MLLPCPYCGSDVIPGADECESCGQTLTDNHLPVPATSVERALLN
                     DRVKLFDGRKPLVVSPTMPVREVLRLMVDNRVGCVTVVENKQLVGIFTERDVLLKLVE
                     NMADWSDKPVSDFMTANVQSLPPSAKIAFAVHRMDQGGYRHVPVVDEHGQPTAVFSVR
                     DILSYLTRAMAVGKQTT"
     gene            complement(2552..2866)
                     /gene="rplU"
                     /locus_tag="VFV87_11795"
     CDS             complement(2552..2866)
                     /gene="rplU"
                     /locus_tag="VFV87_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5104490.1"
                     /translation="MYAIIATDGKQYKVQEGDELQIDLRPDAKEGDQITFDKVLLLSD
                     GTSPKVGRPTVAGATVPAEVLGQEQGEKIYIQKIRRRKNYRRRTGHRQMYTRVRIGAI
                     GG"
     gene            3077..>4110
                     /locus_tag="VFV87_11800"
     CDS             3077..>4110
                     /locus_tag="VFV87_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5104491.1"
                     /translation="MATDPAPTTDSTQSPPEAAPPRAAAGPLAAFKRKAEGGESDQQQ
                     PIAVPLPTETAAASPAAPPAGRGPRPKGGGGKSRRQKEKDEDAQVDKELAAERDARRE
                     FAGRTHVPVPNLRHRSDELEAEVEAALGGMSLDEIVAGPVAPVASRLPNESRHRGQVI
                     EVHNDDVFVALGGKNQGVASLRSFSQPPEAGDMIDVVVTGFNSEDNLHELAIPGGAIV
                     AGDWTDVAEGSLVEATVTAANTGGLECTVKGARAFIPASQVAMYRVENFGDYVGQKLV
                     CVVTESNERRGNLVLSRRAVLEREKEESKKSLLASLQPGDMREGTVRKIQDFGAFVDL
                     GGVDGLIHVS"
BASE COUNT          695 a         1361 c         1346 g          708 t
ORIGIN      
        1 gctgcccgcc ccccgagagc cgcccccggc ggcaggagta tcgccagctt ttcagtacgc
       61 ttcgcaacaa gggatagcga ggcaggcgag atctgcccgt catccatcgc tgcttcgacc
      121 ccaagcgacc tgagtccggc ctcatggccc gcatgctcca agccctgaaa aacctcgagg
      181 cccggtcgcc caagccggcg ccggtggccg gggtcttgtc gcacttggat gccacgcgcc
      241 cgcctgccga gcctgccggg gcccagcttg cccacagccg cgtcgcttat gaagccgcgg
      301 cgcccgctgt atctgtcgtg ccgacgatgg tgatggtgga gtctgcctgg caagcgttga
      361 aggcccccta tgagccgatg gtggtggaaa cgccgcctcc catcgctccc gcggcgccgc
      421 ctgctgctgc cgagccgccg agggtcgaac cgccaactgc tgaggccagg cctgttgttc
      481 cggcgacccg cccgcccacc ggggcggaac gcgccgtccg ccgcacgctc ggcgaaccgc
      541 atcgccgcca gcccctggcc gatcttgcca gccgcttgcg gcaggacgct catcagaccg
      601 gcagccgcac gctgctcttg gtgggtattg gtgccgaaag caccacacac gaaacggcgc
      661 tgcacgtggc ggctttgatg gcggaggaag ggggccgggt gctgctcatc gatgccgatt
      721 tggcccggcg gcagctttcg accgatctgg actccctcca ggagccggga ttttccgatc
      781 tggcccgcga gaacgtatcc gagcagccca ccgcccagcc gacggccttc gaggggctcg
      841 ccttccttcc cgccggccgc cagcggttca tcgatctgga aaactcccgc gccgaccggc
      901 tggcgcgggc cctgggccaa ctttcggccg aatacgatct ggtgctgctc agcggaggcc
      961 aggccaacga tccggcggct ggcccactgg gccgcctcag cgatgctact tacatcgtcg
     1021 tggagctcgg caccgtcgaa gcgggccagg cccagcaggc gctccgcgag ttgcgagcgg
     1081 gcggcgcccg agtcctgggc tgtatcgcca ccggcgtcgc ggcgtaaact cgggactcca
     1141 tgcccaccga tgcttccgcc gcaaacgcgc ccgctgatga acggcggatt ctcatttcga
     1201 tcgcaacgta caacgaaatc gaaaacctcc ctgcaataat cgagaaaatc tggcaggtcg
     1261 cacctcgggc cgacatcgtg gtgatcgacg acgactcgcc cgacggcacc ggccgctggt
     1321 gcgacgagaa ggccagcggc gaaccgcggc tgcgctgcgt gcatcgcccg agcaagctcg
     1381 ggctgggatc ggcgatcctc gcggcgatgc agtacgcgac cgagcacgac tacgattatt
     1441 tgctgaacat ggacgccgat ttcagccatc atccgcgcta tattccggcg ctgctggaag
     1501 tgatgcacag cgctgacgtt gctatcggat cccgatattg tcacggcggc acgacaaagg
     1561 actggcccgc cacaagaaag ctcatgagtc gggccgtcaa tgcctatgcc cgctggctcc
     1621 tgtggctcca gcctaaagac accagcggcg gctttcgctg ctatcgcgtc gcgctgctcc
     1681 aatcgctcgc accacaggcc gtcctcagcc gcgggtactc gtttcagatg gaggtcctct
     1741 ggcggctgaa gcgcttgggc gcccggttgg cggaaacgcc gatcgaattc gccgatcgcg
     1801 agcgcggtca gtcgaaaatc tccaagcagg aggccctgtc ggccctgtgg attatcttcc
     1861 ggctgggcat cacccactgg attggcctgt agcttggctg gttcgcccct gctgaggctg
     1921 cacccatcgc agtcttccgc tagaatgcgt acgcttgtct gctcgcaatc gcgaagcgct
     1981 ggcccgtgag gcgagcgcgg cggagtttac ctatgctgct gccctgtcct tactgtggat
     2041 ccgatgtcat tccaggcgcc gacgagtgcg aaagctgcgg ccagacgctg accgacaacc
     2101 acctgccggt gccggcgacg tcggtcgagc gggccctgct caacgaccgc gtcaagctct
     2161 tcgacgggcg caagccgctc gtcgtttcgc ccacgatgcc ggtccgcgaa gttctccgcc
     2221 tgatggtcga taaccgcgtt gggtgcgtga ccgtcgtgga gaacaagcaa ctcgtcggga
     2281 tcttcacgga acgcgacgtc ctgctcaaac tcgtcgagaa catggccgac tggagcgaca
     2341 agccggtctc ggatttcatg accgccaacg tccagtcgct tcccccctcc gccaagatcg
     2401 ccttcgccgt gcatcgcatg gaccagggcg gctaccggca cgtgccggtg gtcgatgagc
     2461 atggccagcc gaccgccgtt ttctccgtcc gcgacatcct gagttatctg acgcgagcga
     2521 tggcggtcgg aaaacagacc acttgaggcg gctagccgcc aatcgcgccg attcgcacgc
     2581 gcgtgtacat ttgccggtgg ccggtgcggc ggcggtagtt cttgcggcgg cggatcttct
     2641 ggatgtagat tttctcgccc tgttcctggc cgaggacttc ggcaggcacg gtcgcgccgg
     2701 cgaccgtcgg ccggccgact ttcgggctgg ttccgtccga gaggagcagg accttgtcga
     2761 aggtgatctg gtctccttcc ttggcatcgg gccgcaggtc gatctgcaac tcgtccccct
     2821 cctgtacttt gtattgcttt ccgtcggtgg cgatgatggc gtacatggct ttccgttggc
     2881 ggcttgtggc gaacgatggg cgattgagtt gttccgtgac caaccgcgga aacctcgtag
     2941 tttaggcgac gggttaagac tcgtcgaggg gcaccttggc ccgcttctat gaggcttttg
     3001 cggcaagagg taaacttacc cgagtgaggc gaatcgtctc ccgattttaa acgaattttt
     3061 gggaacgcga cctgctatgg cgaccgatcc tgctcctacg accgactcca cccaatcccc
     3121 gccggaggcc gcgccgccgc gggccgcggc cggaccgctg gccgccttca aacgcaaggc
     3181 cgagggcggt gaatctgacc agcagcaacc gattgcggtt ccgctgccca ccgaaactgc
     3241 ggcggcttcg cccgcggccc ctccggcagg gcgcgggccg cggccgaagg gaggcggcgg
     3301 gaagtcgcgc cggcagaagg agaaggacga ggacgctcag gtcgataagg aactggccgc
     3361 cgagcgcgat gcccgccgcg aattcgccgg ccggacgcac gtgccggtgc cgaatcttcg
     3421 ccacagatcc gacgaactgg aagccgaggt cgaagcggct ctgggtggga tgtcgctcga
     3481 cgagattgtc gccgggccgg tcgctccggt cgccagccgc ctgccgaacg agtcgcggca
     3541 ccgcgggcaa gtgatcgagg ttcacaacga cgatgtgttc gtcgcgctgg ggggcaagaa
     3601 tcagggagtc gccagcctgc gcagcttcag ccagccgcca gaagcgggcg acatgatcga
     3661 cgtggtcgtg accggcttca acagcgaaga caacctgcac gaactggcga ttccgggcgg
     3721 agcgatcgtc gcgggcgact ggaccgatgt ggccgagggc tcgctcgtcg aagcgaccgt
     3781 caccgcggcg aacaccggcg gcctggagtg tacggtgaaa ggcgcgcggg cattcatccc
     3841 ggccagccag gtggcgatgt accgcgtgga aaacttcggc gattacgtcg ggcagaagct
     3901 cgtctgcgtc gtcaccgagt cgaacgagcg ccgcggcaat ctcgtcctca gccgccgggc
     3961 cgtgctggag cgcgagaagg aagaatcgaa gaagagcctg ctcgcttcgc tgcagccggg
     4021 agacatgcgc gaggggaccg tccgcaagat tcaggatttc ggggcgtttg tcgatctggg
     4081 aggcgtcgat gggctgatcc acgtcagcca
//
LOCUS       DASUGB010000615         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_486191, whole genome shotgun sequence.
ACCESSION   DASUGB010000615 DASUGB010000000
VERSION     DASUGB010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486191"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFV87_11805"
     CDS             complement(<1..409)
                     /locus_tag="VFV87_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104492.1"
                     /translation="MAIDVTCAGCKTRFQVSDKFAGKQGPCPKCKAIIKVPSKSEQVV
                     IHAPEESGPKSVTGQPVFKPIAWKETRLTTPQIIVIVASVSVVLLATVAFRIAFVGNP
                     PPIVLIVGSILLAPPLSFAGYAFLRDTELEPHRG"
     gene            638..781
                     /locus_tag="VFV87_11810"
     CDS             638..781
                     /locus_tag="VFV87_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104493.1"
                     /translation="MGTKFMKTGNRPIPSLEFPGYGPAVSKELESPRDLPPFVAIPNT
                     WVR"
     gene            complement(904..2325)
                     /locus_tag="VFV87_11815"
     CDS             complement(904..2325)
                     /locus_tag="VFV87_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5104494.1"
                     /translation="MSTLKIPVLRWGKPYESLEVDEVKHFATGEPIAKVSQANGGIIG
                     RDMRTAQKAREILRQIPISELLEKVKKAGELYEKGTLPIGDGTQTPDEFAHQQSASTG
                     LPEHMCKMNMSKNAFVLANMGKILDCLTRGLPLDIFTKGWGEEGRGVVVSYQAQTPVL
                     SAVLPSNSPGVHTLWLPAVPLQMGLVLKPGPQEPWTPYRMIAAYIQAGIPAEVFCVYP
                     GGPEAGAAVLEHCTRAMIFGGTATVERYKGNPRVQAHGPGFSKIVLGDDEVDNWPKYI
                     DLMAESIYINSGRGCINASGVWASRHTKEIAQALAEKLGPIEVKPPTDPAAGLAAFTV
                     PGQAKSVWDMIEADLKESGVTDMTSKYGPRLVEQERCGYLRPVIVHCSSPEAAIAKKE
                     YMFPFATVVECPQDQMLAKMGGTLVCTGITKNQQLIDALSNDIHIDRLNIGPIPTTKL
                     NWLQPHEGNIIDFLYRNRAYQVA"
     gene            complement(2411..2713)
                     /locus_tag="VFV87_11820"
     CDS             complement(2411..2713)
                     /locus_tag="VFV87_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104495.1"
                     /translation="MANFIPTEQLKASDLPEPMADWHSIVRFASTFDIVSEPFKEDIG
                     WGAENASPEMSLPALRAVIHAEWRRYNHRSDLPSQETMRQVWSVIEWIRAAVSQSS"
     gene            complement(2747..3190)
                     /locus_tag="VFV87_11825"
     CDS             complement(2747..3190)
                     /locus_tag="VFV87_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5104496.1"
                     /translation="MSPVLIFDTDHLVILQRESEAESSRIRDKMSRFATSDFFVTIVS
                     FHEQVAGWYDHLAKAKKQRDVVFAYGMFREVLTDFKALQVLDYYDLAAATFASFVKQR
                     IRVGTMDLRIASIAVANDMTLLTRNTVDFQRVPGLRFEDWTLQRG"
     gene            complement(3187..3405)
                     /locus_tag="VFV87_11830"
     CDS             complement(3187..3405)
                     /locus_tag="VFV87_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104497.1"
                     /translation="MSIEERLAVLEEKVESLTKRLELPRKPDWTKRVIGSFANCPEFD
                     EIVRLGREFRQRQNQIPAFEEFEDESAD"
BASE COUNT          680 a         1006 c         1047 g          712 t
ORIGIN      
        1 tgccacgatg cggctcgagt tcggtgtcac gcagaaaggc atagcccgcg aatgacaatg
       61 gcggggcaag cagaattgat ccgacgatga gcacgatcgg cggtggattg cctacaaagg
      121 cgatgcgaaa ggcaaccgtc gcaagaagta cgacggaaac tgacgcaacg ataacgatga
      181 tttgcggtgt ggtaagacgg gtttccttcc aggcgatcgg cttgaagacg ggctgtccgg
      241 taacgctttt gggaccagac tcttccggag cgtgaatgac gacctgctca ctcttcgagg
      301 ggaccttgat gatcgccttg cacttggggc aaggcccctg cttgccggca aacttgtcgc
      361 tgacctggaa gcgggtcttg cagccggcac aggtaacgtc gatagccatt tggggaggag
      421 tcaggggaca ggagtcaggg gacagcggcc ggcgaacaac accatgcgcc acgcacatca
      481 ttgcacggcc agtttcccac acggcagggc agcgtcaagg ttgcgagtct ccgccgtagc
      541 tggcggattc gaccagccgt cgttgtagct tgccctgaat gcggcagtcg tttagcctca
      601 cagcgagcca gccatctaca gtctgacgga gcaccagatg ggtacaaagt tcatgaagac
      661 cggtaaccgg ccgattccgt cactggaatt tccgggttac ggcccggcgg tcagcaagga
      721 attggaaagc ccgcgcgacc tgccgccgtt tgtggcaatt cccaacactt gggttcgctg
      781 aaggcactgg tgccgaagaa gaagcgcatg gtggggttgg gaatgcgcgt tgcgatcgaa
      841 ataccacaga gggagttccc tataggtgtc tgatttctga ccgccgacca ttgactccat
      901 ccctcacgcc acctgatacg cccgattccg gtacaggaag tcgatgatgt tgccttcgtg
      961 cggctggagc cagttgagtt tggtggtggg gatggggccg atgttgaggc ggtcgatgtg
     1021 gatgtcgttc gagagggcgt cgatgagctg ctggtttttc gtgatgccgg tgcagacgag
     1081 cgttcctccc atcttggcga gcatctggtc ctgggggcat tcgacgacgg tggcgaacgg
     1141 gaacatgtat tccttcttgg cgatcgccgc ttcggggctc gaacaatgaa cgatcaccgg
     1201 ccgcagatag ccgcatcgct cttgctcgac gaggcgcggg ccgtatttgc tcgtcatatc
     1261 ggtcacgccg gattccttca gatcggcttc gatcatgtcc cagacgctct tggcctggcc
     1321 ggggacggtg aacgccgcga ggccggcagc cggatcggtg ggcggcttga cctcgatcgg
     1381 cccgagcttc tcggccagcg cctgggcgat ttccttcgtg tgccgcgagg cccacacgcc
     1441 cgaagcattg atgcagccgc ggccggagtt gatgtagatg ctctcggcca tcaggtcgat
     1501 gtatttcggc cagttatcga cctcgtcgtc gccgagcacg atcttgctga agcccgggcc
     1561 gtgagcctgc acgcgcgggt tgcccttgta gcgctcgacc gtcgccgtac cgccgaagat
     1621 catcgcccgc gtacaatgct ccagcaccgc cgcgcccgct tcggggccgc cgggatagac
     1681 gcagaagacc tcggccggaa tgcccgcctg aatgtacgcg gcgatcatgc ggtacggcgt
     1741 ccacggctct tgcgggccag gcttgagcac caggcccatc tgcagcggaa ccgccggcag
     1801 ccagagcgta tgcacgcctg gcgagttcga gggcaagacg gcgctgagca cgggggtctg
     1861 ggcctgataa ctgacgacca cgccgcggcc ttcctcgccc cagcccttcg tgaagatgtc
     1921 gagcggcaag ccgcgggtga ggcaatccag aatcttgccc atgttggcca agacgaacgc
     1981 gtttttggac atgttcatct tgcacatgtg ctcgggcagg ccggtgctgg ccgattgctg
     2041 gtgggcgaac tcgtccggcg tctgcgtgcc gtcgccaatc ggcagcgttc ccttctcgta
     2101 caactcgccc gccttcttca ccttctccag cagctcgcta attggaatct gccgcagtat
     2161 ctcccgggcc ttctgagccg tccgcatatc gcggccaata atcccgccat tcgcctggct
     2221 gaccttggca atcggctcgc ccgtggcaaa atgcttcacc tcatccactt ccagcgactc
     2281 ataaggcttg ccccaacgga gcacggggat tttcaacgtg gacatggggc gttctcccgg
     2341 aaaggtgggc gggcggtggg caaaatcaat atggcgatta ttgttcagac aggtattcag
     2401 tggcaaggtg ctacgatgat tggctcaccg cggctcgaat ccattcgatg acagaccata
     2461 cttgccgcat cgtttcctga gatgggaggt cgcttcgatg attgtagcgg cgccattctg
     2521 catgaatgac ggctcgaagc gcgggcagcg acatttcagg ggacgcattt tctgctcccc
     2581 aaccgatgtc ctctttgaag ggctctgaca cgatatcaaa tgttgaggca aaccgcacga
     2641 tcgaatgcca atccgccatc ggttcgggaa ggtctgaagc ctttagttgc tcagttggga
     2701 tgaaattggc catctacaca tcgcgatcaa acacatgtgg cgcacgtcaa cctctttgca
     2761 aagtccaatc ctcaaaccgc aatcccggca cgcgctggaa atcgaccgta ttgcgcgtga
     2821 gcagtgtcat gtcgttcgcg acggcaatcg aagcgatccg tagatccatc gtgccgaccc
     2881 gaatccgctg cttgacgaat gaggcgaaag tggccgcggc caagtcgtaa taatcgagta
     2941 cctgcagcgc tttgaagtcg gtcagcacct cacgaaacat gccgtaggca aatacaacgt
     3001 cgcgctgctt cttggccttg gcgaggtggt cgtaccagcc ggcgacttgc tcgtggaagg
     3061 agacaatcgt gacgaagaag tcgctcgtcg cgaagcggct catcttatcg cgaatgcgag
     3121 acgattccgc ttcgctctcg cgctgcagga tgaccagatg atccgtgtcg aagataagca
     3181 cagggctcaa tccgccgatt cgtcttcgaa ttcctcaaac gccgggatct gattctgcct
     3241 ctggcgaaac tcgcggccca ggcggacgat ttcgtcaaac tcaggacagt tggcgaacga
     3301 cccgatgacg cgcttcgtcc aatctggctt gcgcggaagt tccaatcgct tcgtcagcga
     3361 ttcgactttc tcttccaaga cggccagtcg ttcttcaatg gacatggcgt gttccttgtt
     3421 cggacgacag ccaattctac cgcgc
//
LOCUS       DASUGB010000616         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1736997, whole genome shotgun sequence.
ACCESSION   DASUGB010000616 DASUGB010000000
VERSION     DASUGB010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736997"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="VFV87_11835"
     CDS             <1..1975
                     /locus_tag="VFV87_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5104498.1"
                     /translation="ALTWVHDNRNAFDNPITTVNLSLGTDWNANTLPQWATLEDELRQ
                     LASDGIFVSVAAGNSFLVYNAPGLSYPAVSQYVTPVASVDSSGNLSRFSQRHNRVLAA
                     PGERIMSTLPDHFYGGDGNKNDWGATSGTSMAAPYVAGASVLVREAMQNLGFTQITQK
                     TIYDLFHSTADTIFDAATNANYKRLNLGRALETLVGADDYASSEELAGSLGSLQTTMH
                     VGGTIGRLNDADFFRFTAASTGVATLSLTGPEHLAAAWKHVGAGRIEGNKLVLDVVAG
                     QTYTVGIEGGGAAIGKYQADLALKATPPAANSGVSIVGNTAQIVGTAGNDTFEWRGGP
                     EAAIVVNGVSYSLAGIASIQISGRGGEDSLTLIGSAATETAVLRPGRVDFAGGGVRLS
                     GEEVERIRVIGGGSDRALLYDSAGNDLLEARPQMVHQSGAEFDHVVEGFGAVSAVAFA
                     GGNDTARLYDSAGNDTLEGGPTSVTLRGTGFKNEARGFEQVFAYATAGNDRASLRDSQ
                     GSDTLDADPNFAWLRGSGFSIRAEGFASVTVLSTSEGTDLARLAGSAGSDEFTILGGT
                     RQLISGNTQIRTDGFQTVAFDGRGGDDSLEFFTASDHSLLSARRDSGAIADALFATEF
                     TNVESVLASVRQSHRLAEDLQAVDFFYRRVGRR"
     gene            complement(1999..>2486)
                     /locus_tag="VFV87_11840"
     CDS             complement(1999..>2486)
                     /locus_tag="VFV87_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX5104499.1"
                     /translation="TVPYRRALYMKARGPGMVFAWRGQEFETDVRHECTHALLHAWLP
                     SVPLWLDEGLAEYFEAARDRRASDQSHLQQVQLLVRSVRLPRLEELEAIDDLDQMGAN
                     EYRDAWAFTHFLLHGPREGTEELLRYLADLEAGENIGPLSARLRRRMLDLDRRLAEHF
                     R"
BASE COUNT          431 a          815 c          802 g          438 t
ORIGIN      
        1 ggccctgacg tgggttcatg ataaccgcaa tgcgttcgac aacccgatca cgaccgtcaa
       61 tctttcgtta ggcaccgatt ggaacgcgaa cacgctcccg cagtgggcca cgctcgagga
      121 tgaactgcgg cagctcgcct ccgacggcat cttcgtctcc gtcgccgccg gaaactcgtt
      181 tctggtgtat aacgcccccg gcctttcgta tccggcggtc agccaatacg tcacgccggt
      241 cgccagcgtc gatagctcgg gcaatctcag ccgcttcagc cagcgccaca accgcgtctt
      301 ggccgcgccc ggcgaacgga tcatgagcac gctgcccgat catttctatg gcggcgacgg
      361 gaacaagaac gattgggggg ctacgtcggg aacgagcatg gcggctcctt atgttgccgg
      421 ggcgagcgtg ttggtgcgcg aggcgatgca gaatctcgga ttcacgcaaa tcacgcagaa
      481 gacgatctac gacctgtttc acagcacagc cgatacgatt ttcgacgccg ctaccaatgc
      541 gaattacaaa cggcttaacc tgggccgcgc gctggagacg ttggtgggcg ccgatgacta
      601 tgcttcgtct gaggagcttg cgggttcgct gggctcgctg caaaccacga tgcacgtggg
      661 cggcaccatt ggccgcctga acgacgccga cttcttccgt ttcacggctg cgagcacggg
      721 cgtcgcgacg ctttccctga caggccccga gcacctggcg gccgcctgga agcacgttgg
      781 cgcgggacga atcgaaggca acaagctggt tctcgatgtc gtcgctgggc agacctatac
      841 cgtcggcatc gaaggtggcg gcgccgcgat cggcaagtac caagccgatc tggccctcaa
      901 agcgacgccg cccgccgcta actcgggcgt gtcgattgtg ggaaacacgg cccagattgt
      961 cggcacggct ggcaatgaca ccttcgagtg gcgcggcgga ccggaggccg caatcgtggt
     1021 caacggcgtg agctattcgc tggccggtat cgcgagcatc cagatctcgg gccggggcgg
     1081 tgaggattcg ctgaccctga ttggctccgc ggcgacagag accgctgtgc tgcgcccggg
     1141 acgcgtcgat ttcgctggcg gtggcgttcg attgagcggc gaggaggtcg agcggattcg
     1201 cgtgatcggc ggcgggtccg accgggccct cctgtacgac tccgccggca acgacctgct
     1261 cgaagcccgc ccgcagatgg tccaccagag cggcgcggaa ttcgaccacg tggtcgaagg
     1321 ctttggcgcg gtgtcagccg tcgcctttgc cggtggaaac gatacggccc gcctctacga
     1381 ctcggccggc aatgacacgc tcgaaggcgg ccccacgtcg gtgacgctgc gcggcacggg
     1441 cttcaagaac gaggcccgcg gatttgagca ggtgttcgcc tacgctacgg ccggtaacga
     1501 ccgggccagc ctgagagact cgcagggcag cgacacgctc gacgccgatc cgaactttgc
     1561 ctggctccgc ggcagcggat tcagcattcg ggccgagggt tttgcgagcg tcaccgttct
     1621 ttcgacctcc gaggggacgg acctggcacg gctcgctggc tcggcgggca gcgacgagtt
     1681 cacaatcctc ggcgggacgc gccaactcat cagtggaaac acacagattc gcacggatgg
     1741 cttccagacc gtcgccttcg atggccgcgg cggggacgat tcgctggagt tctttaccgc
     1801 cagcgaccac agcttacttt ccgcccgccg cgacagcggc gcgattgccg atgcactgtt
     1861 cgccacggag ttcacgaatg tcgaatccgt gctcgccagc gtgcgtcaga gccaccgcct
     1921 ggccgaagac ctgcaagccg tcgatttctt ttaccgccgc gtcggccgcc gctaagtggg
     1981 gcaggcattc ttgcccgcct accgaaaatg ctccgccagc cggcgatcca ggtctagcat
     2041 ccgccgccgc aggcgggcgc tgagcgggcc gatgttctcg cccgcttcca ggtcggccag
     2101 gtatcgcagc agttcttcag ttccttcgcg cgggccgtgc agcaggaaat gcgtaaaggc
     2161 ccaggcgtcg cgatattcgt tcgcacccat ctggtcgaga tcgtcgatcg cttccaattc
     2221 ctccaatcgc ggcagccgga ccgagcggac gagcaattgg acctgctgca ggtgcgattg
     2281 gtcgctggct cgccggtcgc gcgcggcctc gaagtattcg gccaggcctt cgtcgagcca
     2341 cagcggaacg ctcggcagcc aggcgtgcaa cagcgcgtgc gtacactcgt gccgcacgtc
     2401 ggtctcgaat tcttgcccgc gccaggcaaa caccatcccc ggcccgcggg ccttcatgta
     2461 cagcgctcgc cggtatggca ccgtag
//
LOCUS       DASUGB010000617         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_624310, whole genome shotgun sequence.
ACCESSION   DASUGB010000617 DASUGB010000000
VERSION     DASUGB010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624310"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /locus_tag="VFV87_11845"
     CDS             <1..1999
                     /locus_tag="VFV87_11845"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5104500.1"
                     /translation="VVLIISSARVLAQEQSADSWETLQREFSSEVRPLLVHYCNDCHS
                     GDEAKEKIDFESIGDPTTRSSDLATWKRVWDILQAREMPPADEAQPTSPERSKLNDWL
                     LRVLTTPAEGQRPNPGHVVARRLNQVEYNNTLFELFGFNRPPTYFDPQRGMPEQVRLV
                     LHRNFRPVIVDLPPDDVGYGYDNIGEILSLPPFLMEKYLGAAKQVTSLATLRTPPARG
                     DVPYRSSVFNRLRESGDESRGAAKLFLGSFIRRAFRRPVAEAEIERYLKLFDEARNEE
                     ESFEESIAAAIQAVLVSPHFLFRIEHGDAAREEQDIRPLTDFELATRLAYFLWSSTPD
                     DELLRLAEAGQLRDPAVLEAQARRMLRSRKAKELAENFALQWLQVKNIAGAMPDPDRF
                     PQFYRGKNLPEALRQEPLLLFETILVEDRSVLELIDADYSWVNLSLGTFYGLDPDQSG
                     LPDNSLFWVRAPLADKRRGGVLTMGATLVATSSPARTSPVKRGKWILETILGAPPPPP
                     LDNVPDLDSTPATEDGLALREKLARHRSDAACASCHRRMDPLGLSLENYDAIGRWRDH
                     EGPLPIDATGTMSDGSRFSGPAELKQLIVERRRDDFIRCLAQHMLTYALGRKLEHYDL
                     ETIHQIARRVADDDYRLSRLVVEIVCSYPFRYLKTAEVQDE"
     gene            1992..>2369
                     /locus_tag="VFV87_11850"
     CDS             1992..>2369
                     /locus_tag="VFV87_11850"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5104501.1"
                     /translation="MNSRSWKIHRRTALKGLGVTLGLPLLEAMTSVAAANAPAGKPPV
                     RLLFIFVPGGVNVDAWTPRGEGADYEASPTLAALAPVRSDVLVLSGLNGRQGELGGNG
                     HPLGTAPWLSSAPINDRDRGGYAT"
BASE COUNT          410 a          755 c          780 g          424 t
ORIGIN      
        1 cgttgtgttg atcatctctt cggcgcgggt gttggctcag gagcaaagtg ccgattcctg
       61 ggaaacgctc cagcgggagt tctccagcga ggtccgcccg ctattggtgc actattgcaa
      121 cgattgccat agcggcgacg aggccaagga gaagatcgat ttcgagagca tcggcgatcc
      181 aaccactcgc tcgtcggacc tggcgacctg gaagcgggtc tgggacatcc tccaggcccg
      241 cgagatgccg ccggcagacg aggcccagcc gaccagcccg gagcgatcga agttgaacga
      301 ttggctgctg cgcgtcctga cgacgcccgc cgaggggcag cggcccaatc cgggccacgt
      361 tgtggcccgg cggctcaatc aggtcgagta caacaacacg ctgttcgagt tgttcggctt
      421 taaccggcct ccgacctact tcgacccgca gcgcggcatg ccggagcaag ttcgcctggt
      481 gctgcaccgc aattttcggc cggtgatcgt cgatctgccg ccggatgatg tgggctatgg
      541 gtatgacaac atcggcgaga tcctgtcgct gcccccgttc ttgatggaga agtaccttgg
      601 tgcagcgaag caagtcacgt ccctggcaac gctgcggacg ccaccggccc gtggcgacgt
      661 tccctatcgc agttccgtgt ttaatcgctt aagggaatcg ggcgacgagt cgcgtggcgc
      721 cgcgaagttg tttctgggca gcttcatccg cagggctttc cgccgtccgg tcgcggaggc
      781 ggagatcgag cgctacctga agctgtttga tgaggccaga aatgaggagg agtcgttcga
      841 ggagtcgatc gcggcagcga tacaggcggt cttggtctcg cctcacttcc tgtttcgcat
      901 cgaacacgga gacgctgccc gcgaagagca ggacattcgc ccgctgaccg atttcgagct
      961 ggcgacgcgg ctggcgtatt ttctctggag cagcacgccg gacgatgagc tgctgcgatt
     1021 ggccgaggcc ggccagcttc gcgatccggc agtcctggag gcccaggcgc ggcggatgct
     1081 gcggtcgcgc aaggccaagg aactggccga gaacttcgcc ctgcagtggt tgcaggtgaa
     1141 gaacatcgcg ggagcgatgc ccgacccgga ccgcttccct cagttctatc ggggcaagaa
     1201 tttgcccgag gccctccgtc aggagccgct gctgctgttt gaaacgattc tggtcgaaga
     1261 ccgcagtgtg ctggagttga tcgatgccga ttattcgtgg gttaacctct cgctaggcac
     1321 gttttacggc cttgaccctg accagtcggg acttcccgac aatagcctgt tctgggtgcg
     1381 cgcgccgctg gccgacaaac gccgcggcgg cgtgctgacg atgggagcga cgttggttgc
     1441 cacttccagc ccggcccgca ccagcccagt gaagcgcggc aagtggatcc tggagacgat
     1501 cctgggggcg ccgcctccgc cccccttgga caacgtgccc gatctagaca gcacgcccgc
     1561 gaccgaagac ggactcgcgc tgcgcgagaa actggcgcgg caccgcagcg atgccgcctg
     1621 tgcctcgtgc catcggcgaa tggatccgct gggcctgagc ttggagaact acgacgccat
     1681 cggccgctgg cgcgaccacg aggggccctt gccgatcgac gccaccggca cgatgagcga
     1741 cggcagccgt ttctccggcc cagccgagtt gaaacaactg atcgtggaaa ggcgccgcga
     1801 cgatttcatc cgctgcctcg cccagcacat gctcacttac gccctgggcc ggaagctgga
     1861 gcattacgac ctggagacaa ttcaccagat cgcccgccgc gtggccgatg acgactaccg
     1921 gctgtcgcgg ctggtcgtgg agatcgtctg cagctatccg tttcgctacc tgaagaccgc
     1981 ggaggtccaa gatgaatagc cgctcctgga agatccatcg ccgcacggcg cttaaggggc
     2041 tgggcgtgac gctcgggctg ccgcttctgg aagcgatgac gtccgtcgcg gccgcgaatg
     2101 cgcccgccgg caagccgccc gtgcgtctgc tgtttatttt cgtcccgggt ggcgtcaatg
     2161 tggacgcttg gacgccgcgc ggagaagggg ccgactatga agcctcgccg acgctcgccg
     2221 cacttgcgcc ggtgcgcagc gacgtgctcg tcctcagcgg actcaatggc cggcaaggcg
     2281 agctcggggg caatggccac cccttgggca cggccccctg gctctccagc gcgccgatca
     2341 atgaccgcga tcgcggcggc tacgcgacg
//
LOCUS       DASUGB010000618         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2015164, whole genome shotgun sequence.
ACCESSION   DASUGB010000618 DASUGB010000000
VERSION     DASUGB010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015164"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFV87_11855"
     CDS             complement(<1..405)
                     /locus_tag="VFV87_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5104502.1"
                     /translation="MAILQRELKKKRPFDCPEQEALLNLLRTADQLQIHFARLFRRYG
                     LTPQQYNILRILRGEGRPLPILEIASRMITAVPGITGLIDRLVAAELVTRKRCDQDRR
                     IVYVSIAQKATALLAEIDEPLIELHKELLGHLD"
     gene            complement(684..1934)
                     /gene="glgC"
                     /locus_tag="VFV87_11860"
     CDS             complement(684..1934)
                     /gene="glgC"
                     /locus_tag="VFV87_11860"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5104503.1"
                     /translation="MKNTLAVILAGGKGTRLEPLTRDRAKPAVPFGGLYRIIDFTLSN
                     CLNSDIRRVLMLTQYKAMSLDRHINQGWRHYFNRELGEFIDVVPPQQRIDEQWYQGTA
                     DAVYQNIYVLEMERPEHVVILAGDHIYKMDYKAMVEYHKELGADVTVGALRVNPIEAR
                     QFGVMEIDANQRIIGFEEKVQSPKTIPGDPQHCLASMGIYIFTARFLFEQLCRDATRL
                     GSRHDFGRDIIPSLIETHKIYAFPFRDENRKSDAYWRDVGTLDAYFDANMDLTAIDPL
                     LNLYDENWPIRTFQANLPPPKFVFGSRGEADRAGRAMDSVVCQGTIVSGGLVERCILG
                     PRVRVNSYASVADSILFEGVDVGRHARIRRAIIDKGVKVPPGMEIGYDLERDRRRGLT
                     VSEGGIVVIAKGDGLEHMAEPARS"
BASE COUNT          370 a          629 c          740 g          425 t
ORIGIN      
        1 gtcgaggtgg cccagcagct ctttgtgcag ttcgatcagc ggctcgtcga tctcggccag
       61 gagggcggtg gccttctggg cgatgctgac atagacgatt cgccggtcct ggtcacagcg
      121 cttgcgggtg accaattcgg cggccaccag ccgatcgatc agcccggtga tgccagggac
      181 ggcggtgatc atccgggagg cgatttccag gatggggagt ggccgtccct cgccgcggag
      241 gatgcggaga atgttgtact gctgcggagt caggccgtag cggcggaaga ggcgggcgaa
      301 gtggatttgg agctgatcgg cggtccgcag caggttgagc agcgcctcct gctccgggca
      361 gtcgaaaggg cgtttctttt tcagttcgcg ctgcaaaatg gccatcagcg gttcattgct
      421 ccaacagcgg cccggccagg aatgtgccgg taaatcggcc gtctccggcg acggtccgtg
      481 gcggctgctc ctaataattt atacgtcaat gaatagcgag tcaaaatgcg tccgcagccg
      541 gggtatcata ggaacggccg attgccgatc agcaattccg tggactgtgg tggctgaaac
      601 gtggcggctg aaacgtggcg gctgaaacat ggcgagtgaa acgtggcgag tgaaacgtgg
      661 cgagtgaaac gtggcggctg gacttagctg cgggccggtt cggccatatg ttctaagccg
      721 tcccccttgg caatgacgac aatccctccc tcgctgacgg tcaatccgcg gcggcggtcc
      781 cgctccaagt cgtagccgat ctccatcccg ggcggcacct tgaccccctt gtcgatgatc
      841 gcccggcgga tgcgagcatg gcggcccaca tcgacaccct cgaacaggat cgaatccgcg
      901 acgctggcat agctgttcac ccgtacccgc gggcccagga tgcagcgctc gaccaggccg
      961 ccggagacga tcgttccctg gcagacgacg ctgtccattg cccggccggc ccggtccgcc
     1021 tcgccgcggc tgccgaagac gaacttgggc gggggcagat tggcctggaa ggtgcggatg
     1081 ggccagtttt cgtcatacag gttgagcagc gggtcgatgg ccgtcaggtc catgttggcg
     1141 tcaaaatagg cgtcgagcgt gccgacgtcc cgccaatagg cgtcgctctt gcggttttcg
     1201 tcgcggaacg ggaaggcgta aatcttgtgc gtctcgatca gcgagggaat gatgtcgcgg
     1261 ccgaaatcgt gccggctgcc caggcgggtg gcgtcgcggc aaagctgctc gaagaggaag
     1321 cgggcggtga agatgtaaat gcccatcgag gccaggcagt gctgggggtc gccgggaatc
     1381 gtcttggggc tttgcacctt ctcctcaaag ccgatgatcc gctggttggc gtcgatttcc
     1441 atgacgccga actggcgggc ctcgattggg ttgacgcgca gggcgccgac ggtgacgtcg
     1501 gcccccagct ccttgtggta ttcgaccatg gccttgtaat ccatcttgta gatgtgatcg
     1561 ccggccagga tgacgacgtg ctcgggccgc tccatttcca gcacgtagat gttttgatag
     1621 accgcgtcgg ccgtgccctg gtaccactgc tcgtcgatcc gctgctgggg cgggaccacg
     1681 tcgatgaact cgcccagttc gcggttgaaa tagtgccgcc acccctggtt gatgtgccgg
     1741 tccaggctca tcgccttgta ctgcgtcagc atgaggacgc ggcggatgtc gctgttgagg
     1801 cagttggaga gcgtgaagtc gatgatccgg tacagtccgc cgaacgggac cgccggcttg
     1861 gcccggtcgc gcgtcagggg ctcgaggcgc gtccccttgc caccggccag gatcaccgcc
     1921 agggtgttct tcatgctgct cctcaggtcg cggaaaatcg gctcgtgcga tttagtgaaa
     1981 gctcggcgcg tgggtcgatt ttagcaatct gccgccggac gtgcgaccgt gccaaaaccg
     2041 gcgtcgctgg ccggcgcttt ccgtcgatct tgcaggcgca atggcgcgga ggcgatgagg
     2101 cgcaaagcgg cggcgaatgt acgatgatgc cactggaaat cctcatctgc gtcgttgcgt
     2161 cttc
//
LOCUS       DASUGB010000619         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_764664, whole genome shotgun sequence.
ACCESSION   DASUGB010000619 DASUGB010000000
VERSION     DASUGB010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764664"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFV87_11865"
     CDS             complement(<1..333)
                     /locus_tag="VFV87_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5104504.1"
                     /translation="MNDIHVVILAAGKGTRMKSQTPKVLHKIGGLTIIERVIRSAAAL
                     MPASITLVVGHGADDVKRSLAKRTTLQFVTQEQQLGTGHALLQTRPLLEGKSGTVVLL
                     SGDVPLLTV"
     gene            complement(429..1325)
                     /gene="rsmI"
                     /locus_tag="VFV87_11870"
     CDS             complement(429..1325)
                     /gene="rsmI"
                     /locus_tag="VFV87_11870"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5104505.1"
                     /translation="MSFDIGPVRSERRPAGQRESKGVLYVVATPIGNLEDITLRALKV
                     LKLVELVAAEDTRRTGNLLRHFGVEASILSVHEHNERTRIDRLVSHLAKGESVALVTD
                     AGTPGISDPGAALVAAVRAAGFRIEPIPGPSAVIAAISASGITSQGFTFLGFPPIRSK
                     DRKQWFADLHAATQSRLAVFYEAPHKVRKTLQELDLLVKRPIIIARELTKIHEEFVRG
                     TPGELAVRFSDPYGEFTVLVPPRDPAEKAANVVSDEDVVATFGQIAESNQAASKRAVA
                     KRVGERLGMTAKQVYGIIERNK"
     gene            1390..>2250
                     /locus_tag="VFV87_11875"
     CDS             1390..>2250
                     /locus_tag="VFV87_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX5104506.1"
                     /translation="MHAAEPLPHFVDDFLSFLYEVHPTGATMDGVHTHDDLIEDYRRA
                     AIDTHVSALAGFARRLDGIPADRLSLREQVEHQIVSSNIRARQFELEAVRSWERNPHV
                     YAETLASSLAAQAIFNYAPETERARRVLSKLRQVPRLVQAARDNIKEPPAIYVKVGID
                     TWRGTMTFIDRDLPRAFAAVDDIHLLGDLDDACKEAVRTIGSYVGDLENDIRPKAKGT
                     FRLGRDRFEQKLRLEEGINLPVDRLLAIAMRELAATQEEFRQLAGRLNGSDPIEAWRK
                     AKHKQHPDPGA"
BASE COUNT          438 a          706 c          665 g          441 t
ORIGIN      
        1 gaccgtcagc aacggaacat ctcctgacag caggaccacg gtgccggact ttccttcgag
       61 caatggccgc gtttgcagca gcgcatggcc ggtcccgagc tgctgttcct gcgtgacgaa
      121 ttgcagcgtc gtgcgcttcg cgagggaacg cttcacgtca tcggcgccgt ggccgacgac
      181 gagcgtgatg ctggccggca tcaaggcagc ggcgctccgg atcacacgct caatgatggt
      241 gaggccgccg attttgtgca gaaccttggg tgtctgcgac ttcatccggg tgcccttccc
      301 ggcggcgagg atcaccacat ggatgtcgtt cacgcgagca gaatccaacc cttcaatgtt
      361 agtccgagag cgagccgaaa ttttgtttcc acgcgagaca cctgatcgcc tattttctgg
      421 catttaacct atttgtttcg ctcgatgatg ccgtaaacct gcttcgcggt cattccgagt
      481 cgttctccaa ctcgcttggc gactgctctt tttgaagcag cctgattcga ttcggctatc
      541 tgaccaaacg tggccaccac gtcttcgtca gaaactacgt tcgcggcctt ttcggccgga
      601 tctctgggcg gaacgagcac ggtgaattcg ccgtaggggt cgctgaagcg gacggccagc
      661 tcgcctggcg taccccggac gaactcctcg tggatcttcg taagctccct ggcgataata
      721 attggtcgtt taaccaataa atcgagttcc tgcagggtct tcctgacctt gtgaggggcc
      781 tcatagaaga ccgccagacg actctgggtg gctgcgtgaa ggtcggcaaa ccattgtttg
      841 cggtctttag acctaatcgg cgggaagccc aagaaagtaa atccttgcga ggtgatacct
      901 gacgccgaaa tcgcggcgat cacagcactg ggacccggga ttggttcgat tcgaaagcct
      961 gctgcccgga cagccgcaac aagagcggct cccggatcgg aaatcccggg agtcccggcg
     1021 tcggtgacca gggcaaccga ttcgcccttt gcaagatgcg aaaccagccg gtcaatacgg
     1081 gtgcgttcgt tatgctcgtg gacgctgaga attgaggctt cgacgccgaa atgacgaagt
     1141 agatttcctg tccggcgcgt gtcttcggca gcgaccagct cgacaagctt caggactttc
     1201 agcgcccgaa gggtgatgtc ctccagattc ccgatcggcg tcgccacgac gtaaagcacg
     1261 cccttcgact cgcgttgccc agccggccga cgctcgctcc ggaccggccc aatgtcgaag
     1321 gacatcagcg gattttaagc tacgattgca aggatggcct ctccctcaac taactgactt
     1381 taggaaggca tgcacgccgc tgaaccatta ccccacttcg tagacgactt tctttccttt
     1441 ctctacgagg tgcaccccac cggcgccacc atggatgggg tccacactca tgacgatctg
     1501 atcgaggatt accgccgcgc ggcgatcgat acccacgtca gcgcgctggc cgggttcgct
     1561 cgtcgtctcg acggcattcc cgccgacagg ctgtcgctgc gcgaacaggt cgagcaccag
     1621 atcgtctcgt cgaacattcg cgcgcgtcag ttcgagctcg aagccgtccg ctcatgggag
     1681 cgcaatcctc acgtctacgc cgagacgctc gcatcgagcc tggccgcgca ggcgatcttc
     1741 aattacgccc cggagacgga gcgcgcgcgc cgggtgttgt cgaagctgcg ccaggtgccg
     1801 cgtctcgttc aggcggcgcg cgacaacatc aaggagccgc ccgcaattta cgtgaaggtt
     1861 ggcatcgaca cctggcgcgg cacgatgacc tttatcgatc gcgatctgcc gcgggccttt
     1921 gccgccgtcg acgacatcca tctgctcggc gacctggacg atgcgtgcaa ggaagcggtg
     1981 cggacgatcg gcagctacgt cggcgacctg gagaacgaca ttcgccccaa ggccaagggc
     2041 acgttccgtc ttggccgcga ccgcttcgag cagaagctgc gcctcgagga aggcatcaac
     2101 ctgcccgtcg atcggctgct ggccatcgcc atgcgcgaac tggccgccac gcaggaagaa
     2161 ttccggcagc tggccggccg gctgaacggc tccgatccga tcgaagcgtg gcgaaaggca
     2221 aagcacaagc agcatcccga ccctggcgcg
//
LOCUS       DASUGB010000620         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_764737, whole genome shotgun sequence.
ACCESSION   DASUGB010000620 DASUGB010000000
VERSION     DASUGB010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764737"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1365
                     /locus_tag="VFV87_11880"
     CDS             241..1365
                     /locus_tag="VFV87_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5104507.1"
                     /translation="MSFVHDRRTFLAAASSLAAAGLVSRRAFADDAADRKKLQELVDK
                     AISFLQTKGQAADGSFTKQVGIGITALAATAMMRHGRSPEDPSVAKALKYLEQNVQPT
                     GGIHAANGRLKTYETCVALLAFVEANRDGKYDEIIKKAEAFIKKIPFDAEEGNSPEDF
                     QFGGAGYGGQGRPDLSNTAYLIEALKAAGAGEDDPAIKNALIFVSRCQNLESPHNTTP
                     FAAKVNDGGFYYTPLPGNDDATTASGGLRSYGAMSYAGLKSMAFAGVKPDDPRVQAVR
                     KWVAKNYDVKTHPGQGEAGLFYYYNTFAKALEANGEAEITDADGKKHNWRKDLIDELA
                     RRQKDDGSWANANQRWFETDPNLSTSFALLALSYCVPEKK"
     gene            1480..>2950
                     /locus_tag="VFV87_11885"
     CDS             1480..>2950
                     /locus_tag="VFV87_11885"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104508.1"
                     /translation="MLRILGSARQLCGGMTRREMLRVGGLGIAGLSLPQILALQEASA
                     DRPLTPGPSPTSGEGTSFGKAKAIILIHLYGSPSQLEWVDCKPEAPAEVRGTFGVIPS
                     SLPDCPVGELFPTMAKVMDRTTVLRSLTHPYPIHGVAYALTGVPAIDVNMELSPHDAK
                     HWPYFGSVVEFVESQRASKAATSGRGPALPPSVPNNIALPFRFSTQRVGEVPRAGPYA
                     AFLGSQYDPLWADFQGKANKGITKTLRDMTYTDPDPYVGIEPGSYFVVPDASSLQPEL
                     TLDRLDRRKNLLTQFNQVLPGLIESTTGRALTKHQQAALSLLESPKLAEALDLRRESV
                     ETRELYGSSLFGQSCLAARRLVEAGSKVVSVFWDEYGLAGSGWDTHWDHFNRMSKELA
                     PGFDVGWYGLITDLDRRGMLDETLVVCTSEHGRTPQINKAQGGGRDHWSRAYSSLLAG
                     GGTHRGKVIGATDKIAGDVVDRPISPKDLLATMYHLLGID"
BASE COUNT          564 a          932 c          964 g          490 t
ORIGIN      
        1 ggtaggttcc gacggcctcg gcggaaccgg atgcgcgagt gacctcagcg ccggtgccgg
       61 tcccgctcag gcggccctaa cctataacca gacggcccta gcctaaaatt cggcgcccgc
      121 gcgcagaatc tgagcgcagc cgctgagaaa acaggcgcct ggcggtacga tagcgggcgg
      181 cacgcaggat gcagcatccc aacgcacttc ccattccacg cccgagcctt ggagtttccc
      241 atgtccttcg ttcacgatcg ccgcacgttc ctcgccgccg cttcgtcgct cgctgccgcc
      301 gggctggttt cccggcgagc gtttgccgac gatgcggccg atcggaagaa gctgcaggag
      361 ctggtcgata aggcaatcag cttcctgcag accaaggggc aggcggcgga tgggtcgttc
      421 accaagcagg tggggatcgg catcacggcg ctggcggcga cggccatgat gcgccacggc
      481 cgctcgccgg aggatccgag cgttgccaag gccctcaagt acctggagca aaacgtccag
      541 ccgacgggcg gaatccacgc ggccaacggc cgcttgaaga cgtatgagac gtgtgtggcg
      601 ctattggcgt tcgtggaagc gaatcgcgac ggcaagtacg acgagatcat caagaaggcc
      661 gaggcgttca tcaagaagat cccgttcgac gccgaggaag ggaacagccc cgaggatttc
      721 caattcggcg gcgcgggcta tggcgggcag gggcggccgg acctctcgaa caccgcgtat
      781 ctgatcgagg cactcaaggc ggccggcgcg ggcgaggacg acccggcgat caaaaacgcc
      841 ctgattttcg tgagccgttg ccagaacctg gaatcgccgc acaacaccac gccgttcgcc
      901 gccaaggtga acgacggcgg cttctactac acgccgctgc cgggcaatga cgacgcgacg
      961 accgccagcg gcggcctgcg gagctacggc gcgatgagct acgccggcct caagagcatg
     1021 gccttcgccg gcgtgaagcc cgacgacccg cgcgtgcagg cggtccgcaa gtgggtcgcc
     1081 aagaactacg acgtgaaaac ccaccccgga cagggcgaag cgggcctgtt ctattactac
     1141 aacacgttcg ccaaggccct cgaagccaac ggcgaagcgg aaatcacgga cgcggacggc
     1201 aagaagcaca actggcgcaa ggatctgatc gacgaactgg cccgccgcca aaaggacgac
     1261 ggctcctggg ccaacgccaa ccagcgctgg ttcgagaccg atcccaacct ctccaccagc
     1321 tttgcgctct tggcactcag ctactgcgtg ccggagaaga agtaggctcc agagattttt
     1381 cccgaatcgg ctggaaaagt cgtcccatct tctcgataat cgagaacgtc agcggacgtt
     1441 gattggtccc cggcctgcgg gccgctcggg aggacggacg tgcttcgcat actcggctct
     1501 gccaggcagc tttgcggcgg catgacgcgg cgggagatgc tgcgcgtggg cggtctgggg
     1561 attgcagggc tgtcgctgcc gcagatattg gcgctgcagg aagcttctgc agatcgtccc
     1621 ctcacccccg gcccttctcc cacgagcgga gaggggacat cgtttggcaa ggccaaggcg
     1681 atcatcctga tccacttgta cggctcgccg agccagctgg agtgggtcga ttgcaagccg
     1741 gaggcgccgg cggaagtgcg cggcaccttc ggcgtgattc cgtcgtcgct gccggattgc
     1801 ccggtgggcg agttgtttcc cacgatggcc aaggtgatgg accggacgac ggtcctgcgg
     1861 tcgctgacgc atccctatcc gattcacggt gtggcgtacg ctctgacggg cgtaccagcg
     1921 attgacgtga acatggagct ttcgccgcac gatgcgaagc actggccgta ctttggttcg
     1981 gtcgtggaat ttgtcgaaag ccaacgcgcg agcaaagccg cgaccagtgg tcgcggtccc
     2041 gctctgcctc caagcgtccc gaataacatt gctttgccgt tccgcttcag cacgcagcgc
     2101 gtcggcgaag tgccccgggc cgggccgtat gcggcgttcc tgggcagcca gtacgatccc
     2161 ttgtgggcgg attttcaagg caaggcgaac aagggaatca ccaagacgct ccgcgacatg
     2221 acctacacgg accccgatcc gtatgttggc attgaacctg gatcgtattt cgtcgtcccg
     2281 gatgcatcga gcctgcagcc ggaactgacg ctcgaccggc tagaccggcg gaagaatctg
     2341 ttgacgcagt tcaatcaggt cttgccaggt ctgatcgagt cgacgaccgg cagggccctg
     2401 acgaagcacc agcaggccgc gctttcgctc ctggaatcgc ccaagctggc cgaggccctc
     2461 gacctgcgac gcgagagcgt agagacccgc gagctatatg gctcttcgct ctttggccaa
     2521 tcgtgtctgg ccgcccggcg gctggtcgag gcggggagca aggtcgtcag cgtcttctgg
     2581 gacgaatatg gcctggccgg gagcggctgg gatacgcact gggatcattt caaccggatg
     2641 agcaaagagc tggcgccggg cttcgatgtc ggctggtatg gcctgatcac cgatctggat
     2701 cgccgcggga tgctcgacga gacgcttgtc gtctgcacca gcgagcatgg ccgaacgccg
     2761 cagatcaaca aggctcaggg aggcggtcgc gaccattggt cgcgagccta ttcgtcgctc
     2821 ctggccggcg gcggcacgca ccgcggcaaa gtcatcggcg cgaccgacaa gatcgccggc
     2881 gacgtcgtcg atcggccgat cagccccaag gacctgctcg ccacgatgta tcatcttttg
     2941 ggcatcgacc
//
LOCUS       DASUGB010000621         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_209736, whole genome shotgun sequence.
ACCESSION   DASUGB010000621 DASUGB010000000
VERSION     DASUGB010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209736"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFV87_11890"
     CDS             complement(<1..687)
                     /locus_tag="VFV87_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate dehydrogenase family protein"
                     /protein_id="HEX5104509.1"
                     /translation="MKVSIIGGGGLVGSSAGFALQCGGVVREIALLDVNQDLAGGQAL
                     DLLHGTPSVADQVITSGGYEHIPSSDIVCITAGLRRKPDESRLDLINRNTDLFLGILS
                     EIAKAKLKHDAIVLVVSNPVDILTYVAAERLDLPPCQVLGLGTQLDTIRFRSLIAQQL
                     QAAPTQVNALILGEHGDSMVPIWSSASIGGLPLEKFPGWTNRLADELFTRTKGSGAEV
                     IKKKTGAGFAV"
     gene            complement(775..1644)
                     /locus_tag="VFV87_11895"
     CDS             complement(775..1644)
                     /locus_tag="VFV87_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5104510.1"
                     /translation="MANLHRLKQDICEIGRRIYAKGFAAANDGNITVRVGANEFLCTP
                     TMHCKGFLKPEDICLIDFTGKQLAGIKKRSSEALLHLEIYRQRDDVKSVVHCHPPHAC
                     AFAIAREPIPQCILPEVEVFLGDVPITKYETPGGQAFADTIQPFVKKTNIILLANHGT
                     VSYGTDVERAYWWTEILDAYCRMLLLSKQLGRVNFFDETKERELLELKAKWGWSDPRN
                     TEEYKNCDICANDIFRESWKNSGVERKAFPAPPPMGPAAKGGSSNGSGGGLDQESLVQ
                     MITERVMEELAKR"
     gene            complement(1755..2033)
                     /locus_tag="VFV87_11900"
     CDS             complement(1755..2033)
                     /locus_tag="VFV87_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX5104511.1"
                     /translation="MRIAKVVGTVTLNRCHPSFVGARLRLVVPLSLAELRGEGQPGAD
                     EMVAWDDNAAGVGDFVAMSEGAEASQPFRPDLKPVDAYAAAILDEVDV"
     gene            complement(2140..>2352)
                     /locus_tag="VFV87_11905"
     CDS             complement(2140..>2352)
                     /locus_tag="VFV87_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104512.1"
                     /translation="RRLGLLDQTAAQSAADLRLEERVITMDSIKGKLAGISRLVVGGG
                     SIITPAVKDELRQRNIELVRQSLRPF"
BASE COUNT          401 a          721 c          723 g          507 t
ORIGIN      
        1 gacggcgaag ccagccccgg ttttcttctt gatgacttct gctcccgagc ctttggtgcg
       61 ggtaaatagc tcgtcagcca gccgattcgt ccagccgggg aacttttcca gcggcagccc
      121 gccgatcgag gcgctcgacc aaatcggcac catgctgtcg ccgtgctcgc cgaggatgag
      181 ggcgttgacc tgtgtcggcg cggcctggag ctgctgggcg atcaggctgc ggaaacggat
      241 cgtgtcgagc tgcgtcccca ggccgaggac ctggcagggc ggcaaatcga gccgctcggc
      301 ggcgacgtac gtcaggatat cgaccgggtt gctgaccacc aggacgatcg cgtcgtgctt
      361 gagcttcgcc ttggcgattt cgctgaggat tccaaggaac aagtccgtat tgcggttgat
      421 caggtccagc cgcgattcgt ccggtttgcg ccgcaggccg gcggtgatgc agacaatgtc
      481 gctcgacgga atatgctcgt agccgccgga ggtgatcacc tgatcggcaa cgctgggcgt
      541 gccgtgcaag agatcgaggg cctgcccgcc ggccaagtcc tgattcacgt caagcagggc
      601 gatctcgcgc accacgccac cgcactggag cgcgaagcca gcgctggaac caaccagtcc
      661 gccgccgccg atgatggaga ctttcatagt cacgagtcag tgggcaggag tcaggagtca
      721 gtaacttgtt tccgcgctcc gcgtggggaa cacacgactg gacgctccgc gtctctaccg
      781 cttcgccagc tcctccatca cacgctcggt gatcatctgg actagcgatt cttgatccaa
      841 gccgccaccc gagccgttgg aggacccacc ttttgccgct ggtcccattg gtggcggggc
      901 gggaaacgcc tttcgctcga cgccgctgtt cttccagctc tctcggaaga tgtcgttggc
      961 gcagatgtcg cagtttttgt attcctccgt gttccgcggg tccgaccagc cccacttggc
     1021 cttcagctcc agcagttccc tctccttcgt ctcgtcgaag aagttcacgc ggcccagctg
     1081 tttggagagg agcagcatcc ggcaataggc gtcgaggatt tcggtccacc agtacgcccg
     1141 ctcgacatcc gtgccgtagc tgaccgtgcc atgattggcc agcaggatga tgttcgtctt
     1201 cttgacgaac ggctggatcg tgtcggcgaa ggcctgtccc ccgggcgttt cgtatttggt
     1261 gattggcacg tccccgagga agacctcgac ctcgggcagg atgcactgcg ggatcggctc
     1321 gcgggcgatg gcgaaggcgc aggcatgcgg cggatggcag tgcacgacgc ttttcacgtc
     1381 gtcccgttgc cggtaaatct ccaggtgcag cagcgcctcg ctcgatcgct tcttgatccc
     1441 cgccagttgc ttgccggtga agtcgatcag gcagatgtcc tccggcttga ggaatccctt
     1501 gcagtgcatt gtaggcgtgc agaggaattc attcgcgccg acgcggacgg tgatgttgcc
     1561 gtcgttggct gccgcaaagc ccttggcata gatgcggcgg ccgatttcgc agatgtcttg
     1621 tttgaggcga tgaaggttgg ccatcgaagg gttcagggtt cggggttcag ggtttggtgt
     1681 tcggggtttg gtgttcgggg ttcggggttc gggcgcgtga ctgcagcatt tcgtagccac
     1741 tagccactaa ccactcaaac atccacttca tccagaatcg ccgccgcgta cgcatcgacg
     1801 ggctttaagt cgggccggaa cggctgcgac gcttcggccc cttcactcat cgcgacgaag
     1861 tctcccacgc cggctgcgtt atcgtcccag gcgaccattt cgtcggcccc gggctggcct
     1921 tcgccgcgca attctgccag cgacagcggc acgaccagcc gcagccgggc cccgacgaaa
     1981 ctggggtggc agcgattgag cgtcacggtg ccgacaactt tggcgattct catgattcga
     2041 ttaacgcagt cggtttaacg caaaggcgca acgaccgcaa agttttcagc tctttgcgtc
     2101 ttggcgtttc ttggcgcctt tgcgtttcat tgatggaaat caaaatggtc ttagtgattg
     2161 gcggaccagc tcgatgtttc gctgtctcaa ttcgtctttc accgcgggcg taatgatcga
     2221 cccgccaccc acaaccaatc gacttatgcc cgccagtttt cctttgatcg aatccatcgt
     2281 gatgacacgt tcctcaagcc gcaggtctgc cgcggattgg gccgccgtct gatccagcaa
     2341 ccccagccgc cg
//
LOCUS       DASUGB010000622         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_210067, whole genome shotgun sequence.
ACCESSION   DASUGB010000622 DASUGB010000000
VERSION     DASUGB010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210067"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..424)
                     /locus_tag="VFV87_11910"
     CDS             complement(155..424)
                     /locus_tag="VFV87_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104513.1"
                     /translation="MKGFSTAIGIILAAIFLAILVVSSVPRSEQRTLPVQITHYLVTG
                     SLVAVLWAICFRRQVEKLRFSLLSLFVLAAMEAVLFAAIRLLNPF"
     gene            complement(476..1366)
                     /locus_tag="VFV87_11915"
     CDS             complement(476..1366)
                     /locus_tag="VFV87_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5104514.1"
                     /translation="MPTTTDIADSAHATEALEAAKLEALYQFAYGLSHEINNPLANIA
                     TRAQTLLLDEKDPERRRKLATINQQAFRAHEMIADLMLFARPPALRLEETDLAALADT
                     IVAELQDQAREQGTRLTRTGEPGPLLATIDPTQIAVALRAIIQNALEAVRTGGSVTVN
                     AECGMRNAEYDVSPPPDPPLSTQYSVLSTQPIPSPHFTPRTPNPEPRTPTAPTPQSAI
                     RNPQFLLTVTDTGPGIPPHIRPHIFDPFFCGREAGRGLGLGLSKAWRIINLHGGQLEV
                     ESPAEGGARFVMAIPGRSPT"
     gene            complement(1502..2107)
                     /locus_tag="VFV87_11920"
     CDS             complement(1502..2107)
                     /locus_tag="VFV87_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104515.1"
                     /translation="MVYAMSATTKTVFTTGEAAKICKVSQQTIIRCFDNGSLKGFRVP
                     GSKFRRIPRNELYAFMRDNGIPTDALESGKRKVLIVDDDEELVELMSDTFARDGRFEI
                     KTANNGFDAGMLVKEFRPDLVVLDIMLPDINGKEVCQRIRSDSTMETVKVICISGMVE
                     ADKVSDLKAAGANDFMQKPFQVERLLDRCCELLDMEKAVLA"
     gene            2657..>3218
                     /locus_tag="VFV87_11925"
     CDS             2657..>3218
                     /locus_tag="VFV87_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104516.1"
                     /translation="MLAIISDLHITDGTTGQLLPAGTMDLLCERLCDLAWRASWRPGG
                     VYRPIDRIDLVLLGDTLDLISSRRWLASPCRPWDDPQSPAVIDTIGGIVEEILRTNVD
                     AIRTLRNLATESTVSLPPATAAGQPVLEAEEIPVAVCTHYMVGDRDWPLHLAGSQYDL
                     IRHKITHHLGLVTPYNKPFPHEAGECE"
BASE COUNT          561 a          961 c         1047 g          649 t
ORIGIN      
        1 cgtcgatgtg cccgacacgc aggggaaccg cgagttcttc tccggcctga aagaacgcct
       61 caaatcccgc ttccagcagc tcgacatctg ggtcaccacg cacccgctgg agattctgta
      121 agaagccgct gaaagtcgtc tgtgcgggat catcctaaaa tggattgagc aggcgtatgg
      181 cagcaaacaa gacggcttcc atggccgcga gcacgaatag cgaaagcaag ctgaagcgaa
      241 gtttctccac ttgccgccgg aagcaaatgg cccacaggac cgcgaccaag gagccggtca
      301 cgaggtaatg ggtgatctgc accggaagtg ttcgttgttc tgagcgcggg acactcgaga
      361 ccacgagaat cgcgaggaag atcgcggcga gaatgatccc aatcgctgtc gagaagcctt
      421 tcatcgagat ggattgtaac cgcgccgatg cttttaccgt ttgcaattgg gcgggctagg
      481 tcggcgaccg acctgggatt gccatgacaa atctcgctcc accttcggct gggctttcga
      541 cttcgagttg cccgccgtgg aggttgatga tgcgccaggc tttggagagg ccgaggccga
      601 ggccgcggcc ggcttcgcgg ccacagaaga acgggtcgaa gatgtgtggg cggatatggg
      661 gcgggatgcc ggggccagtg tcggtaacgg tcaggaggaa ttgcggattg cggattgcgg
      721 attgcggagt gggggcggtg ggggttcggg gttcggggtt cggggttcga ggggtgaagt
      781 ggggcgatgg gatcggctga gtactgagta ctgagtactg agtactgagt ggtgggtcgg
      841 gcggcgggct gacgtcgtat tccgcattcc gcattccgca ttccgcattg actgtcacgc
      901 tgccgccggt gcggaccgct tccagggcgt tttggatgat ggctcggagg gcgacggcga
      961 tttgcgtggg atcgatggtg gcgaggagcg ggccgggttc gcccgtgcga gtgaggcggg
     1021 tgccttgttc gcgggcctgg tcctgcagct cggcgacgat ggtgtccgca agcgcagcca
     1081 gatcggtctc ttccagccgc agggcggggg gccgggcgaa gagcatcagg tcggcgatca
     1141 tctcgtgggc gcggaaggcc tgttggttga tcgtggccag cttgcggcgg cgctcaggat
     1201 ccttctcgtc taatagcagc gtctgcgccc gcgtggcgat gttcgccagc gggttattga
     1261 tctcgtggct caagccgtaa gcaaattggt agagcgcttc cagcttggcg gcttctaggg
     1321 cctccgtcgc atgagccgaa tcggcgatgt ccgtcgtagt tggcatgtcg gacggagaga
     1381 aggagcgaca gagagaagga gagaacatgg cgcatcgtcg ctccgtctct ctgtctctcc
     1441 gtctctcctt cttgcttcct tcttgcggcc atggcgggca ggcgagacgc ctaccccacg
     1501 gctacgccag caccgccttc tccatatcca gcagctcgca gcagcggtcg agcagccgct
     1561 cgacctggaa cggcttctgc atgaagtcgt tggcgccggc ggccttgagg tcggagacct
     1621 tgtcggcttc gaccatgccg gagatgcaga tcaccttcac ggtttccatc gtcgaatcgc
     1681 tgcgaatccg ctggcagact tccttgccgt tgatgtccgg cagcatgata tcgagcacga
     1741 cgaggtcggg ccggaactcc ttgaccagca tcccggcgtc gaagccgttg ttggcggttt
     1801 tgatctcgaa gcggccgtcg cgggcgaacg tgtcgctcat cagctcgacg agctcttcgt
     1861 cgtcgtcgac gatcagcacc ttccgcttgc cgctttccag ggcgtcggtg gggatgccgt
     1921 tgtcccgcat gaaggcgtac agctcgttcc gcggaatgcg gcggaacttg ctgccgggca
     1981 cgcggaagcc cttgagcgat ccgttgtcga agcagcggat gatcgtctgc tggctgacct
     2041 tgcagatttt ggcagcttcg cccgtcgtga acacagtctt tgtggtggct gacatggcgt
     2101 acaccatccc ttccgttacg atggctggac tagctgttgc agaagccgcc gagagcccgg
     2161 ggcgtccgaa acctccgtgt tttgccacgt cgttgctagt ccgaaaactc cctctttgtc
     2221 agaaactgcg tcttgtccgc agttcgcaca gttgtgcggg ttatcgcatc cggcagtctc
     2281 tggcgggttt gccaagcttg ccgaatttga caaccgcgtc gaattatatc ggcgtgaaaa
     2341 accgcgtcaa gattgaattc tctgtcgcaa gtccatgtag cgtcgtgact tagcgccgcc
     2401 agccccaatt gtccgcaaat tctctccagc cggcagattc cctcttatcg gcccattcct
     2461 cgcacaatcc gcagacagtt tttcctgaac aatcctcacg cccggcagaa tcgcgcgccg
     2521 ataagaaacc gaacgtgggg taggcgtctc gcctgcccgg gagattgggt tccgggaacg
     2581 caaccactgg tcgcggcttt gtcaggaacc gcgaccactg gtcgcggctt tgcacgcgca
     2641 ccacaaggac gtggccatgc tcgcgatcat cagcgatctg cacatcaccg acggcaccac
     2701 cgggcagttg cttcccgcgg gcacgatgga cctcttgtgc gaacgcctgt gcgatctggc
     2761 ctggcgggcg tcgtggcggc ccggcggcgt ctatcgcccg atcgaccgca tcgacctggt
     2821 gctgctgggc gatacgctcg atttgatctc gtcgcggcgc tggctggcct cgccctgccg
     2881 cccctgggac gatccgcagt cgcccgccgt gatcgacacg atcggcggca ttgtcgagga
     2941 gatcctgcgg accaacgtcg atgccattcg cacgctccgc aatctggcga ccgaatcgac
     3001 cgtcagcctg ccccccgcca ccgccgccgg ccagccggtg ctggaagcgg aggagattcc
     3061 cgtcgcggtc tgcacgcact acatggtggg cgaccgcgac tggccgctgc acctggcggg
     3121 ctcgcaatac gacctgatcc gccacaagat tacgcaccac ctgggcctgg tcactcccta
     3181 caacaagcct ttcccgcacg aagcaggcga gtgcgagc
//
LOCUS       DASUGB010000623         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_348607, whole genome shotgun sequence.
ACCESSION   DASUGB010000623 DASUGB010000000
VERSION     DASUGB010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348607"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VFV87_11930"
     CDS             <1..1144
                     /locus_tag="VFV87_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5104517.1"
                     /translation="DLSIQLGTPNGCVACHIVDSQLSPAQKPKSPSENRPVEYADWLR
                     DAAEEKPAVKAELERLNQWADAACEKWYGAKRKKEPHFARALLAAREMRPDAQEKLIE
                     LLGKREQPAIARATAAAELGAYVNPAPGYSAVAIRALQEALADKSPEVRAAAAESLGN
                     APPDDVQKLLLPLLADPVRMVRYRAAQALSPFTSEELFSADERTLQTVLREMEAQTTA
                     SDRAGYYLMRAGFAQRRGDLAQAGADYETAIDLEPTLSGPRQNLAALLDQLRQPAEGA
                     RLRREELDLRERDARRAPQIAALQVQVGLARHLNGWKKEAESALLQASLLEPRNPAML
                     EYLAIYYRDTGRPDDALALAERLWKLRPGHPGYERMRQELRDEAGR"
     gene            1302..>2089
                     /locus_tag="VFV87_11935"
     CDS             1302..>2089
                     /locus_tag="VFV87_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104518.1"
                     /translation="MAGGLSLCASATLGNAPENRAAQAKSVLLIYEQGGLSHIDTWDP
                     KPEIPVDHRSPHGVIATKVPGMQFTSLLSKTARVAEKLTVVRSMYHKDGGVNGHPDGT
                     QYALSGEVPGGPLEMPDIGGVVTHRLGSVCNYLPPYIMVPGNHEQAAMTRQGFLPPET
                     AVFKTQGNLADPKWQVPNLGLLAGIDQGRFLDRRDLLSNLHVGVASGAGRRLASMQSL
                     REKTVDMLTNSATTSAFRLAEEPDAMREKYGRGHRGQCYLLGRRL"
BASE COUNT          389 a          676 c          713 g          311 t
ORIGIN      
        1 cgatctgagc atccaactgg ggacgcccaa cgggtgtgtc gcctgccaca tcgtggacag
       61 ccagctttcc ccggcgcaaa aaccaaagtc tccgagcgag aaccgcccgg tcgaatacgc
      121 cgactggctg cgggacgcgg cggaggagaa gcccgcggtg aaggcggagc tcgagcgctt
      181 gaatcagtgg gccgatgcgg cctgcgaaaa gtggtacggc gcgaagcgga agaaggagcc
      241 ccactttgcg agggcgctgc tggcggcccg cgagatgcgc ccggacgcgc aagagaaact
      301 catcgagctg ctcggcaagc gcgagcagcc ggccattgcc cgggcgaccg ccgcggcgga
      361 gctgggggct tacgtcaatc ccgcgcccgg atattccgcg gttgcgattc gggctctgca
      421 ggaagcgctc gccgacaaga gtcccgaagt ccgcgccgcg gcagccgaga gcctggggaa
      481 tgctccgccc gacgacgtgc aaaagctgct gctgccgctg ctggcggacc ccgttcgcat
      541 ggtgcgttac cgggcggctc aggccctgtc tcccttcacc agcgaggagc ttttttccgc
      601 cgacgagcgg acgctgcaaa ccgtgctgcg ggagatggag gctcaaacaa cggcaagcga
      661 ccgggcgggg tattatttga tgagagcggg cttcgcccag cggcgggggg accttgctca
      721 agccggcgcc gactatgaaa ccgccatcga cctggagccg acgctcagcg gcccgcgcca
      781 gaatctagcg gcgctcctgg accagctccg gcagccggcc gagggggccc gcctgcgacg
      841 cgaggagctc gacttgcggg agcgggacgc gcgccgggct ccacaaatcg ccgcgctgca
      901 agtccaggtg ggtctggcgc ggcacctgaa cggctggaag aaggaggctg aatcggcctt
      961 gctccaggcc tcgctgctgg agccgcggaa tccggcgatg ctcgagtacc tggcgatcta
     1021 ctaccgcgac accggccggc cggacgacgc gctcgcgctg gccgagcgtt tgtggaaatt
     1081 gcggcctggg catccgggct acgagcgaat gcggcaggaa ttgcgggacg aagcagggcg
     1141 atagccggga tggatccctg ccgaggggtc ggcccctgag gaattccgct tgccgctgcc
     1201 cgggccctgg gggagaatag atccacccgc cgaatcgtcc cacctcaaac gtcccaccga
     1261 tgaccagacg catgcagcgc cgcagctttc tcggaatagg aatggccgga ggcctttcgc
     1321 tttgcgccag tgcgacgctg ggaaacgccc ccgagaatcg agcagcccag gccaaaagcg
     1381 tcctgctgat ttacgagcag gggggcctgt cgcacatcga cacgtgggat cccaaaccgg
     1441 agattccggt cgatcaccgt agcccgcacg gggtcatcgc gacgaaagtc ccggggatgc
     1501 agttcacgtc gctcctctcc aagacagccc gcgtggcgga aaagttgacg gtggtccggt
     1561 cgatgtatca caaggacggc ggcgtgaacg gtcatcccga cggaacgcag tacgcccttt
     1621 ccggcgaagt ccctggcggg ccgctggaaa tgcctgacat tggcggcgtg gtgacccacc
     1681 gccttggttc cgtctgcaac tatctgccgc cgtacatcat ggtgccggga aaccacgagc
     1741 aggccgcgat gacccggcaa ggctttctgc cgccggaaac cgccgtcttc aagacccagg
     1801 ggaacctggc ggaccccaag tggcaggttc ccaacttggg cctcctcgcc ggcatcgacc
     1861 agggtcgatt tctcgaccgg cgggacctgc tcagcaatct ccatgtcggc gtggccagcg
     1921 gtgcgggccg gcgattggca tcgatgcaat ccctccgtga gaaaacggtg gacatgctca
     1981 ccaatagcgc cacgaccagc gcgtttcggc tggccgaaga gcccgatgcg atgcgcgaaa
     2041 aatatggccg cggccatcgc gggcaatgtt acctgctggg acggcgtct
//
LOCUS       DASUGB010000624         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_625605, whole genome shotgun sequence.
ACCESSION   DASUGB010000624 DASUGB010000000
VERSION     DASUGB010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625605"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..625)
                     /locus_tag="VFV87_11940"
     CDS             complement(44..625)
                     /locus_tag="VFV87_11940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5104519.1"
                     /translation="MVQFLLVRPGSTDFDEQGRIKGTLDIPLSDTGVGQVADIVAQLR
                     GTPIDFLYASPTRCAQQTAQAIAHDRHLRFKTIDDLENLDHGLWQGKRIDEVKTSQPK
                     VFRQLQEHPETVCPPQGEPIGSAIERVREVLARLVRKHRGVTVALVVPEPLASLVRSL
                     LCRCEMPDLWKAECEGGGWELIDISAQPVALAS"
     gene            complement(647..1333)
                     /gene="rpe"
                     /locus_tag="VFV87_11945"
     CDS             complement(647..1333)
                     /gene="rpe"
                     /locus_tag="VFV87_11945"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5104520.1"
                     /translation="MTRRDRLAELREAAPVVLPSLLLCDFGNLEREIRNLEAAGVRAL
                     HLDVMDGNFVPNLTYGMPIVEACRRLTELPIDVHLMISEPQKYLRQFYEAGADNLTFH
                     VEAVADPRPILNEIHALGAGAGLALNPATPLSAVEPALDLCDVVLVMSVPAGFGGQSF
                     HPVAIDKLRQLRQIARPDILLEVDGGISGKTIAPCAQAGAQLFAVGSAIFSHPDYGRA
                     VSELTSLARI"
     gene            complement(1625..>2182)
                     /locus_tag="VFV87_11950"
     CDS             complement(1625..>2182)
                     /locus_tag="VFV87_11950"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5104521.1"
                     /translation="LIRGEGADVCEITPRSILEAILFVGHPTAEPLTSERIAALMRGV
                     RPEEIDDLVRQLNEEYAAEGAPYAIVSVGPGYQLALRPEFSPLRDHFYGRLREARLSQ
                     AAVDCLAIVAYHQPIATGEIDRLRGKPSGHILSQLVRRDLLRIDRPAERHAKPTYSTT
                     ERFLDLFGLEDLQELPRSQEIEREL"
BASE COUNT          416 a          666 c          709 g          391 t
ORIGIN      
        1 agtgcggcta cccacaatga caacaaagct accagcccgc ggcttagctt gccagcgcaa
       61 ccggctgggc ggaaatgtcg atcagctccc agccgccccc ttcgcactcg gctttccaga
      121 ggtcgggcat ttcgcaccgg cagaggagcg agcggacgag gctggcgagg ggttcgggaa
      181 cgacgagggc cacggtcact ccgcgatgtt tgcggacgag ccgggccagc acctcgcgga
      241 cgcgctcgat cgccgagccg atcggctcgc cttgcggcgg gcagacggtc tcggggtgtt
      301 cctgcaattg gcgaaagacc ttcggctggc tggttttgac ttcgtcgatg cgcttgccct
      361 gccacaggcc gtggtcgagg ttttcaaggt cgtcaatcgt cttgaaccgc agatggcgat
      421 cgtgggcgat ggcctgggcg gtctgctgcg cacagcgcgt gggcgaagcg tacaggaaat
      481 cgattggcgt gccgcggagc tgggcgacga tatcggccac ctggccgacg cccgtgtcgc
      541 tgagggggat gtcgagcgta cctttgatgc ggccctgctc gtcgaaatcg gtggagccgg
      601 gccgaaccag gagaaactga accatacggg gtgtctggaa acctctctaa atgcgggcca
      661 gcgacgtgag ttcgctcacc gcccggccgt agtcaggatg ggagaaaata gccgaaccaa
      721 ccgcaaacag ttgtgctccc gcctgagcac agggtgcaat tgtcttgccg ctgatgccgc
      781 cgtcgacttc caggaggatg tcagggcggg cgatttggcg aagttggcga agtttatcga
      841 ttgcaacggg gtggaacgac tgcccgccaa agccggcagg cacactcatc accaatacca
      901 catcgcacag gtccagtgca ggctccaccg ccgacagggg agtggcgggg ttcagcgcca
      961 agccagcgcc agcccccaaa gcgtggattt cgttgagtat gggccgcgga tcggccaccg
     1021 cctcgacgtg gaaggtcagg ttgtccgccc cggcttcgta gaactgccga aggtacttct
     1081 gcggctcgga aatcatcaaa tggacatcga tcggcaactc cgtcagtcgc cggcaggcct
     1141 ccacgatggg catgccatac gtcagatttg gcacgaaatt gccgtccatg acgtccagat
     1201 gcaaagcacg gacccctgcg gcttcgaggt tgcgaatttc ccgctccagg ttgccaaaat
     1261 cgcacagcaa gagcgacggc aagaccacag gggcggcttc gcgcagctcg gccaggcgat
     1321 cgcggcgggt cataggcggc gagtgcggct gcgcggtaat aaccactgcc gacatcctct
     1381 gtggagttcg atccgctcgt gtgcggggcc cacgcgtcgc agtcagcggc cgaaatggcg
     1441 cgtcacggtc ggtgcgacag gcttaattct acctttggtt tcggagggcg tcagtagctg
     1501 ttgtagggcc tgcgctgatg aactccccga aaggcaggcg aagaaaagcg agatccacaa
     1561 gacgacgcaa gtctatatgt ttcaatatct tatagcgaca tatttccacg agaccgcggt
     1621 tgccctacag ctcccgctcg atctcctggc tccgcggcag ctcttggagg tcttcgagac
     1681 cgaacaggtc gaggaagcgc tcggtcgtcg agtaagttgg cttggcgtgc ctttcggccg
     1741 gccggtcgat cctgagcagg tcgcgccgca cgagctgcga gaggatgtgg ccgctcggct
     1801 tgccgcgaag gcggtcgatt tcgcctgtcg cgatcggctg gtgataggca acgatcgcca
     1861 aacaatcgac cgccgcctgg ctcaggcggg cctccctcag ccgaccatag aaatggtccc
     1921 gcagcgggct gaattcgggc cgcagggcca gttgatagcc cggaccgacg gaaacgattg
     1981 cgtacggcgc accttccgcc gcatattcct cgttcaattg ccgcaccaag tcgtcaattt
     2041 cctccggtcg cacgccccgc atcagggcgg cgatccgctc gctcgtgagg ggctcagcgg
     2101 tggggtggcc gacgaacaag atcgcctcca gaatgctccg cggcgtgatt tcgcacacat
     2161 cggcgccttc accgcgaatc ag
//
LOCUS       DASUGB010000625         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_488533, whole genome shotgun sequence.
ACCESSION   DASUGB010000625 DASUGB010000000
VERSION     DASUGB010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488533"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VFV87_11955"
     CDS             complement(<1..1468)
                     /locus_tag="VFV87_11955"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5104522.1"
                     /translation="MARRWPLSLCGVVLATCFAWCSLRMAAAAEDSADGVEFFEKKIR
                     PVLVEKCYKCHSAGAEKLEGKLHLDSREGSRKGGETGSAVVPGDAEASLLLKAIRYED
                     IEMPPSGKLPAEVIADFERWINAGAVDPREDAGAPPAAKQIDFAAGRQFWSFQPPRFH
                     DLPAVSRPEWLSRKSDAFVLAKLDAAGLTPAEAADRRTWLRRVTLDLTGLPPTPEDAA
                     AFETDPSPDAHERVVDRLLAAPQHGERVARMWLDLARFAEDQAHIVGNDASLTYPNAY
                     LYRDWVIGAINRDLPYDEFVRLQLAADLVEGDESPNVPALGLLGLGPKYYSRRSLAVM
                     SDEWEDRVDIVGRGLLGLTVACARCHDHKFDPIGTEDYYALAGIFASTAMFNKPLRDD
                     VEKAGDKKDGEDKEFENGQKLAAEAKNPKDALHVVREGSPTDLNVFIRGNVENKGPLV
                     PRHFLRVLCGGEPAAFASGSGRRELAEAIASPENPLTAR"
     gene            1647..2792
                     /locus_tag="VFV87_11960"
     CDS             1647..2792
                     /locus_tag="VFV87_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5104523.1"
                     /translation="MNELEQNSETTERSSKQHPRWPLMVLAVLAILAGLYFARDVLVP
                     VAFAVMLTLLLSPLLRKLRRWRIPDLLSALLLVAGTAAIFVLGVALLAGQAQYWLAEA
                     PVTVRKVRDLLPSGLGPLSDLAATTDAVQSITNDGAAERPVKVEIQSWDLTLTVVGES
                     SHFVASALITFVLAFFLLAFSDTLLRQAASSCASFNEKRNVVELIYNIENGVSRYLRT
                     VTLINIGLGIVAGLALWMLGVPNPLLWGVMVAILNFVPHVGAFVCLVVLFFVGAVTRE
                     SITYGLLCAGIFVVLTSVESYFVTPICLSKSLQLSPLAVILAILFWGWLWGVAGGLMA
                     APLLAIVKIACDQFEPLRAWSAILAGVPTLKAPQQQEIPVNRAASVA"
     gene            2920..3480
                     /gene="frr"
                     /locus_tag="VFV87_11965"
     CDS             2920..3480
                     /gene="frr"
                     /locus_tag="VFV87_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5104524.1"
                     /translation="MAADDVLLDVDDRMDKAIQHLKQQLSGIRTGRANPGLVDTLKVE
                     VYGSLSPMKQLASVGAPEPQQIVIRPYDPGTIKEIERAIVNSGLGFAPQNDGRIIRIN
                     VPPLTTETRKKLVARIKELTEEAKVSIRNVRRDGNKAGEQGEKDGDLTEDELKTLKDD
                     IQEMTKKYEGIASDMAKKREDEVMEG"
     gene            3561..4451
                     /locus_tag="VFV87_11970"
     CDS             3561..4451
                     /locus_tag="VFV87_11970"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5104525.1"
                     /translation="MQNTGGACSLDAWLLPVFVFACVLVISDAADSAEPTPAQKLVDG
                     PRVLIVAHRGDSGAFPENTLPAFQSAVDVKADFVELDYYHSADGVPVVIHDDVLDRTT
                     NAKLLLGKEKLAVAKLPLADLQKLDAGSWFGQKFAGAKLPTLIESLDLIQTGSTTLIE
                     RKAGDAKTLVKLLDDKKLTDRVVVQAFDWEFVAECRKLSPRLVLGALCGKPADEEKIR
                     GAVATGADVIVWNHEKLEKEHIDLIHQLGKRAWVYTVDDPDRARWLLKAGIDGIITNR
                     PAEMRALRERSQGSGVRKPQ"
     gene            complement(4458..5054)
                     /locus_tag="VFV87_11975"
     CDS             complement(4458..5054)
                     /locus_tag="VFV87_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104526.1"
                     /translation="MGFSVSWLAIRGKPLDSVLDELGLRSTGEFADVPDSPIVGTLVP
                     TGWFVVYYNRIIGDGDVKALSAGCEIITCDIEEHVMYSGAAYWRNCRQIWSVVHQSVQ
                     GLDHLRTVGELPLEFVAIRDKLITESRLTRGTELAADYVFDIPIELAKAITGFRHDED
                     IEGAEEDPYQVLERKRQPRRDWNFQSILAVLGWRRSNA"
BASE COUNT         1045 a         1604 c         1573 g         1024 t
ORIGIN      
        1 cgcgggccgt cagcgggttt tcgggcgaag cgatcgcctc ggccaattcg cggcgcccgc
       61 tgccgctggc gaaggccgcc ggctcgcccc cgcacagaac ccggaggaaa tgtcgcggca
      121 cgagagggcc tttgttctcg acattgccgc gaatgaacac attcaggtcg gtcggcgagc
      181 cctcgcggac cacgtgcagc gcgtctttgg gatttttggc ctccgctgca agcttctggc
      241 cgttctcgaa ttctttgtcc tcgccatctt tcttatcgcc cgccttctcc acatcgtcgc
      301 ggaggggctt attgaacatt gccgtgctgg caaagatgcc ggccagcgca tagtaatcct
      361 cggtgccgat cggatcgaac ttgtgatcgt ggcagcgggc gcaggccacg gtcagaccca
      421 agaggccgcg gccgacgata tcgacccggt cctcccattc gtccgacatc acggccagac
      481 tccggcgact gtagtacttt ggccccaggc cgagtaaccc caacgctggc acgttcggcg
      541 attcatctcc ctcgaccaaa tcagcggcaa gctgcaggcg gacgaactcg tcgtacggca
      601 agtcgcgatt gattgcgcca atcacccagt cgcgatacag atatgcgttc ggatacgtca
      661 gcgaggcatc gttgccgacg atatgcgcct ggtcttccgc gaagcgggcc aggtcgagcc
      721 acattcgcgc cactcgctcg ccgtgctgcg gcgcggccag cagccgatca acgacccgct
      781 cgtgagcgtc gggagatgga tcagtttcga aagcagcagc gtcctccggt gtcggcggca
      841 ggcccgtcag atccaacgtc acccgccgca gccacgtccg gcggtccgcc gcttcggccg
      901 gcgtaagtcc ggctgcatcc agcttcgcca ggacgaaagc atcactcttg cggctgagcc
      961 attcaggccg gctgacggct ggtaaatcgt ggaatcgcgg cggctggaac gaccagaact
     1021 gccggccggc tgcgaaatcg atttgcttcg cggccggcgg cgcgccggcg tcctcgcgag
     1081 gatcgaccgc cccagcgttg atccaccgct cgaaatccgc gatcacttcc gccggcagct
     1141 tgccgctcgg aggcatctcg atatcttcat agcgaatcgc cttcagcagc aggcttgcct
     1201 cggcatcgcc cggtacaacc gccgagcccg tctcgccccc cttccgcgac ccctcgcgac
     1261 tgtccaagtg cagcttcccc tcgagcttct ccgccccggc tgagtggcac ttgtagcatt
     1321 tctcgacgag caccgggcgg atcttcttct cgaaaaactc aacgccatcg gccgaatctt
     1381 cagcggccgc ggccatgcgc aatgagcacc aggcgaagca cgtcgccagc actacgccgc
     1441 acaggctaag gggccaacgt cgagccatcg gtttgcggca aggcaagaaa gggggggaag
     1501 ttcaggcggg aagctatttc tagcatcccc atcgcataca ctcaagcaaa tctcatccag
     1561 cccgcgtctc gccatctgct gccattgatc tcactcgatc tcacacttcg ccggacaggc
     1621 atggcgtttg cgtgtacggc gcgtgcatga atgaacttga gcaaaactcc gaaaccaccg
     1681 agcgatccag caagcagcac ccacgctggc cgttgatggt tttggccgtc ctggcaatcc
     1741 tagctggctt gtatttcgct cgcgatgtgc tcgttcccgt agcgtttgcg gtaatgctca
     1801 cgctgctctt atcgcctctc ttgcgcaaac tgcgtcggtg gcgaatcccg gatctcctat
     1861 ctgcgctact tcttgtcgcg ggtacggcag cgatcttcgt cctgggtgtg gcgcttttgg
     1921 ccggccaggc acagtactgg ctggctgaag caccagtaac agtgcgaaag gtgcgggacc
     1981 tgctgccttc aggactcggg ccgctcagcg atttagcagc gacgaccgac gccgtgcagt
     2041 caatcaccaa cgatggtgca gccgagcgac ctgtcaaggt ggagattcag tcctgggact
     2101 taaccctgac agtcgtgggc gagagcagcc actttgtcgc gtctgccctg ataacctttg
     2161 tgctggcttt ctttcttctg gctttcagcg atacgctctt gcggcaggcg gcgagctcgt
     2221 gtgcgtcgtt caacgagaaa cggaatgtcg tcgagttgat ctacaacatc gagaacggcg
     2281 tttcgcgata cttgcggacg gtgacgctga tcaatattgg gctcggtatc gtcgcaggac
     2341 tggcgctgtg gatgttgggc gttcccaatc ccttgctgtg gggtgtgatg gtcgccatat
     2401 tgaatttcgt gcctcacgtt ggggcgtttg tctgcctggt cgtgctgttt ttcgtgggag
     2461 ccgtgacgcg cgaatcaatc acttacggcc tgctgtgcgc cggaatcttc gtcgtgctga
     2521 cgtcggtcga aagctacttc gtgacgccga tatgccttag caagtcgctg cagctctcgc
     2581 cgctcgccgt catcctggcg attttgtttt ggggctggtt gtggggcgtc gcgggggggt
     2641 tgatggcggc tccgctcctg gcgatcgtga agattgcctg cgaccaattt gagcccctgc
     2701 gcgcctggag cgctattttg gcgggcgtgc ccacgctgaa ggcgcctcag cagcaggaga
     2761 ttccggtcaa ccgcgctgcg agcgtcgcct gagggccgct gccattgcgg ctgggcggga
     2821 agcgttcggt tgcacgtatc cgtttgttaa caaacgaccg gcgagtcgct agaattgcgc
     2881 cgttgacatc agacactgat ttgacccgca ggtgctctta tggctgctga tgatgttctc
     2941 ctggacgtgg acgaccggat ggacaaagcg atccagcacc tcaagcagca gctatcgggc
     3001 atccggacgg gtcgggcgaa cccggggctg gtcgatacgc tgaaagtcga ggtctatggc
     3061 tcgctctcgc cgatgaagca gctcgccagc gtcggtgctc cggagccgca gcagatcgtg
     3121 atccgcccct atgaccctgg gacgatcaag gaaatcgagc gagcaatcgt caacagcgga
     3181 ctcggctttg ccccgcagaa cgacggacgg atcatccgca tcaacgttcc accgctgacg
     3241 accgagacgc ggaagaagct ggtggcgcgg atcaaggagc tcaccgagga ggccaaggtc
     3301 agcattcgca acgtccgccg cgacggcaac aaggccggcg aacagggcga gaaggacggc
     3361 gacctgaccg aggacgagct caagacactg aaggacgaca ttcaagaaat gaccaaaaag
     3421 tacgaaggaa tcgccagcga catggcgaaa aaacgcgaag acgaagtgat ggaaggctga
     3481 caaagccgcg accagtggtc gcggttccca acgacctgga accgacgaat cggacgaatc
     3541 aaaccaatga ggcggaagcg atgcagaata ccggtggtgc ttgttcgctc gatgcgtggc
     3601 tgcttcctgt cttcgtcttc gcctgtgtgc ttgtgataag cgatgccgct gattccgcgg
     3661 agccgacgcc ggctcaaaaa ctggtggacg gaccgcgcgt attgattgtc gcccaccgcg
     3721 gcgattcggg cgcgttcccg gaaaacacat tgccggcgtt tcaatcggct gtggatgtga
     3781 aggccgattt cgtcgagctg gactactacc actcagccga cggcgtgccg gtggtgattc
     3841 atgacgacgt tttggatcgc acgaccaacg cgaagctgct actgggcaag gagaaactcg
     3901 ccgtcgccaa gctgccgctg gccgatttgc agaagctcga cgcggggagc tggtttggcc
     3961 agaaattcgc cggtgcaaag ctgccgacgc tgatcgaatc gctcgatctt atccagaccg
     4021 gctcgacgac gctgatcgag cgtaaagcag gcgacgcaaa aacgctcgtc aagctgctcg
     4081 acgacaagaa gctgaccgac cgggtcgtcg ttcaggcttt cgactgggaa ttcgtcgccg
     4141 agtgccgcaa gctctcgccg cgcctcgttc tcggcgcgtt gtgcggcaaa ccggctgacg
     4201 aagagaaaat ccgcggcgca gtcgccacag gcgccgacgt catcgtctgg aatcacgaaa
     4261 agctggagaa ggagcacatc gacctgatcc accagctagg caagcgggcc tgggtctata
     4321 cggtcgatga tcccgaccgc gcccgctggc tgctgaaagc cgggattgac gggatcatca
     4381 ccaatcggcc ggcggagatg cgggcgctgc gcgagaggag tcagggatca ggtgtcagga
     4441 aaccgcaata gctcgaatca cgcattcgat cgtctccatc caaggactgc cagaatgctt
     4501 tgaaaattcc agtcgcgccg tggttgtcgc ttccgctcaa gtacttgata tgggtcttct
     4561 tctgctccct cgatgtcctc gtcatggcga aagccggtga ttgcctttgc aagctcgata
     4621 ggaatatcga aaacatagtc ggcagcgagt tcggttccgc gtgtgagccg cgattcagtt
     4681 atcaacttgt ctcgaattgc cacgaactcc aacggcaatt caccgactgt gcgaaggtgg
     4741 tcaagcccct ggaccgattg gtggacgacg gaccagattt gacgacaatt gcgccagtat
     4801 gccgcgccag aatacataac gtgctcctca atatcgcaag tgatgatttc acatccggct
     4861 gaaagcgctt tgacatctcc atcaccaatt atgcggttgt agtacacgac gaaccaccca
     4921 gtgggtacga gcgttcccac aatcggcgag tctggaacgt ctgcaaactc gccagtcgag
     4981 cgaagcccaa gctcgtccaa caccgaatct agtggtttgc cgcggatcgc taaccaggaa
     5041 acggagaacc ccacgcccgc gctcctcgcg ctacgtcacg cccgacaatc gctgaaagag
     5101 atcgcctacg cgacacagaa aacccgggac gacaccttct ccgtcaagag tgtcgtcggc
     5161 agcgagaata acgccccggc catcgggatg atgcacaaat acttggcgcg tcaccgggtc
     5221 gatcacccat acgagccgca cgcccg
//
LOCUS       DASUGB010000626         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_625828, whole genome shotgun sequence.
ACCESSION   DASUGB010000626 DASUGB010000000
VERSION     DASUGB010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625828"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFV87_11980"
     CDS             complement(<1..434)
                     /locus_tag="VFV87_11980"
                     /inference="COORDINATES: protein motif:HMM:NF025460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HEX5104527.1"
                     /translation="MSRLSRPSAAQVAERLVVLKFIVAHAMIAPPRDMLNEWMRSWPP
                     HEYAGFEREATARNKAFWQKALDAGLWEELSPEELELASATIVTMTQQQQVNASWRIE
                     AAQVLMWALELIPELPPYDTQASHDLLREIPKMPDFAAQARLR"
     gene            665..1639
                     /locus_tag="VFV87_11985"
     CDS             665..1639
                     /locus_tag="VFV87_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104528.1"
                     /translation="MYLRSTALTVVALLAPCCLFAADDNPAYTNAKEAGPDFAVQGEY
                     LGAETGDANNKWGAQVIALGDGKFDVVGYKGGLPGAGWQRGDESKRGTGELKDGVLSL
                     KGDDWTASVKDGVFTVVHEGQTVAELKKIERKSPTLGAQPPQGAIVLFDGTTAENFEN
                     GQIIEENLLAASNPVSKLKMGDHTLHLEFRTPFMPKSRGQARGNSGVYPQGRYEIQIL
                     DSFGLEGKNNECGGIYSISEPAVNMCLPPLSWQTYDIEFTAAKYDGDKKIANARATVK
                     HNGVVIHDNVDLKDGTPGKDQEGPTKNGLYLQNHGNPAAFRNIWVVEK"
     gene            1676..>2798
                     /locus_tag="VFV87_11990"
     CDS             1676..>2798
                     /locus_tag="VFV87_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104529.1"
                     /translation="MAAIIAGAEEPASPPVAPLRVVTDDYHGTRIDDPYRYFENQADP
                     EVVRWFKGQADHAAAVLARIPGRDSLLARLREIDQATAYRITDIQPRANGDLYYFKQL
                     ADEALPKFYHRSAAGQEALLIDPARHDGADEQHASLTYCVPSPDGQHIAYGIAQGGSE
                     ETTIYVWDVAAGKDLADVIDRIETAYTEPQWLPDGSGFYYVRRQKLPSGTPPTEIYKR
                     TQALFHRLGEDPERDVLVLAQDSSPDVPLAEADFPSLALTPGSRFAIGKIKHGDAADM
                     TLYSALLSDLGTPRVKWRRICDASHKVIDFAVRGDDVYLLTADNAPRYKIVRTSLTAP
                     DFAKAEPVVPPGEATIASLAVAQEALYVGLRFAARGEVRR"
BASE COUNT          545 a          886 c          875 g          492 t
ORIGIN      
        1 cgaagtctgg cctgcgcggc gaaatcgggc atcttgggaa tttctcggag caagtcgtgg
       61 ctcgcctgcg tgtcatacgg aggcagttcg ggaatcaact ctagcgccca catcaggacc
      121 tgtgcggctt cgatgcgcca cgaagcgttg acttgctgct gctgggtcat cgtcacgatg
      181 gtggcactgg ccaattccaa ttcctcggga gacagctctt cccacaggcc agcatctagc
      241 gctttctgcc agaatgcttt gttgcgcgcc gtggcctccc gttcaaatcc ggcgtattcg
      301 tgcggcggcc aactccgcat ccattcgttt agcatgtccc tcggtggagc aatcattgca
      361 tgcgcgacga tgaacttcag gaccactagg cgctcggcaa cttgtgcggc actgggtcgc
      421 gagagtctgg acatttatct tagggcgagg ccggtcgagg ctgacgcgtt cgcaacaatc
      481 aggatagctc atcgtacacg gctacgggca tacgggttcc tcttctctgt tgcggtgcag
      541 agaagaggaa cccgtattat tgttcgtggc cgcctcgggt ctgttgccgc accggccgat
      601 ccgcgccaca atagcatcgc tcaaacatga actttgaccg tccgcttcgc ctaggagacc
      661 cgccatgtac ttgcgctcga cggcactgac agtcgttgcc ttgcttgccc cttgctgcct
      721 gtttgccgcc gacgacaacc cggcttacac caatgccaag gaagccgggc ccgatttcgc
      781 cgtgcagggc gaatacctcg gcgcagaaac gggcgatgcg aacaacaaat ggggcgcgca
      841 ggtcatcgcg ctgggcgacg gcaagttcga tgtcgtcggc tacaaggggg gcctgcccgg
      901 cgcgggctgg cagcggggcg atgaatcgaa gcgtggcacg ggcgagctga aggacggcgt
      961 gctgtcgctc aaaggcgacg actggacggc aagcgtcaag gacggcgtgt tcaccgtggt
     1021 gcacgagggg cagacggtgg ccgagctcaa gaagattgag cgcaaaagcc ccacgctcgg
     1081 cgcccagccg ccccaagggg caatcgtgct cttcgacggc acgaccgccg agaatttcga
     1141 gaatgggcaa atcatcgaag agaacctgct ggcggcgagc aatccggtga gcaagctcaa
     1201 aatgggcgat cacacgctgc acctggaatt ccgcacgccc ttcatgccca agtcgcgcgg
     1261 ccaggcccgc ggcaacagcg gcgtctatcc gcaggggcgg tacgagattc agattctcga
     1321 cagcttcggc ctggaaggga agaacaacga gtgcggcggc atctattcca tctcggagcc
     1381 ggcggtcaac atgtgcctgc cgccgctctc gtggcagacc tatgacatcg agttcaccgc
     1441 ggccaagtac gacggcgaca agaagatcgc caatgcccgc gcgacggtta agcacaacgg
     1501 cgtcgtgatc cacgacaacg tcgatctgaa agacggcacg cccggcaagg accaggaagg
     1561 ccccaccaaa aacggcctgt acctgcaaaa ccacggcaat ccggcggcct tccggaatat
     1621 ctgggtggtg gagaagtaag ccccgcccat gaacgccctc ccaggatgga tttgcatggc
     1681 agcgatcatc gccggcgccg aagagcctgc ttcgccgccg gtagcgccgc tgcgcgtggt
     1741 caccgacgac tatcacggca cgcggatcga cgatccgtac cgctatttcg agaatcaggc
     1801 cgatccggaa gtggtgcggt ggttcaaggg gcaggccgac cacgcggctg cggtcctggc
     1861 cagaattccc ggccgcgatt cgctgctggc ccgcctgcgc gaaatcgacc aggccacggc
     1921 ctatcgcatc acggacatcc agccgcgggc caatggcgac ctgtattatt tcaagcagct
     1981 tgccgacgaa gcgctgccca agttctacca ccggtccgct gctggacagg aagcgctgct
     2041 gatcgatccg gcacggcacg acggcgcaga cgagcagcac gcctcgctga cctactgcgt
     2101 tccgtctccg gacggccagc acattgccta cggcatcgcc cagggaggct cggaagagac
     2161 cacgatctac gtgtgggacg ttgcagccgg gaaggatctg gcggatgtga tcgaccgcat
     2221 cgagacggcg tacaccgagc cgcagtggct tcctgacggc agcggctttt actacgttcg
     2281 ccgccagaag ctgccgtccg gcactcctcc gaccgagatt tacaaacgaa cgcaggcgct
     2341 ctttcaccgg cttggcgaag atcctgaaag ggacgtgctc gtgctggccc aggattcatc
     2401 gccggatgtg ccgctggccg aagccgattt ccccagcctc gcgctcactc ccggatcgcg
     2461 gttcgcaatc ggcaagatca agcacggcga tgcggccgac atgacgctgt actcggcctt
     2521 gctaagcgat ctgggcacgc cgcgcgtcaa gtggcggcgg atctgcgacg cctcgcacaa
     2581 ggtcattgat tttgccgtcc ggggtgacga cgtctatttg ctcacggcag ataacgcgcc
     2641 gcgctacaag atcgtccgca cttcgctgac cgcgcccgat ttcgcgaagg ccgagccggt
     2701 ggttccgccg ggcgaagcca cgatcgcgtc gctggctgtg gctcaggaag cgctgtatgt
     2761 cggcttgcgc ttcgctgccc gcggcgaagt ccgccgcc
//
LOCUS       DASUGB010000627         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1876387, whole genome shotgun sequence.
ACCESSION   DASUGB010000627 DASUGB010000000
VERSION     DASUGB010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876387"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFV87_11995"
     CDS             complement(<1..503)
                     /locus_tag="VFV87_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104530.1"
                     /translation="MNATCPLNRLRADYPWPSARPGVRPIEWSLDAGGKHLVIEQIRR
                     RRATLVLEIGSFLGASVRTWLAASPDVVVIALDPWPDIRRWLPELNQKGTQPAWAIEQ
                     LATGEDAAYHTFLANLWDERQRVIPMRDAAPAALYNLQKLGVQPELIYLDADKTGAEL
                     EVCRELFP"
     gene            complement(718..1521)
                     /locus_tag="VFV87_12000"
     CDS             complement(718..1521)
                     /locus_tag="VFV87_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104531.1"
                     /translation="MHVRELVELAALVAVHGPILVRSGQRVPHSAIEQYWVASKCRID
                     RWLRLLRRLADAAGQFPRPATLAWPRIGPACEEILASELLTRIWTAAAVACDEHHADE
                     NLSPIARNVWLGHLDVRRRLLQLVADGRAFTPEQATKINHLRRRLERWNDMLLAHLSS
                     LPGTAEFAFDPARAGDFAEDLDPATTFADRSFTCQLVQASLTASLGGILQGSSPNADL
                     NRQIASAIFACLPDDLLAGRQSAGSLWLDRLVRTAAHAQQMVDDLVRLG"
     gene            1846..2331
                     /locus_tag="VFV87_12005"
     CDS             1846..2331
                     /locus_tag="VFV87_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5104532.1"
                     /translation="MRIGVISDTHGHVTNTLAAVRLLESLDVEAVLHCGDIGTPEIPK
                     LLATWPTHFVFGNCDHETGELKEAISAAGLTCHGRFADLELGGRKIAVLHSDDARRFR
                     ETIFSGRYDLVCYGHTHEAESHREGQTLVLNPGALYRAQPHTLAVVNLPTMEVEIVTV
                     E"
     gene            complement(2338..2811)
                     /gene="rimI"
                     /locus_tag="VFV87_12010"
     CDS             complement(2338..2811)
                     /gene="rimI"
                     /locus_tag="VFV87_12010"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5104533.1"
                     /translation="MIRRDMPEVLAIESQSFEFPWSEEDFVRCLRQRNCIGMVAEHNE
                     RVVGFMIYELHKNRLHILNFAVNEQLRRRRVGSQMVRKLASKLSRERRNRIMLEIRET
                     NLAAQLFFRAAGFRAISVLRAFYEDTPEDAYLMQFRYSAAAAASATEAQPTRMAG"
BASE COUNT          596 a         1056 c         1096 g          555 t
ORIGIN      
        1 gggaacaact cgcggcaaac ttccagttcg gctcccgttt tatctgcatc gagataaatc
       61 agctcgggct gcacgccgag cttttgcaga ttgtagagcg ccgcaggcgc ggcgtcgcgc
      121 atcggaatca cgcgctgccg ctcgtcccac aggttggcga gaaacgtgtg gtaggcggcg
      181 tcctcgccag tggccagttg ctcgatcgcc caggccggct gtgtgccctt ctggttcagc
      241 tccggcagcc agcggcgaat gtccggccag ggatcgaggg cgatgaccac cacgtccggc
      301 gaagcagcca gccacgtccg caccgatgcg cccaggaacg acccgatctc cagcaccagc
      361 gtggctcgcc gccgccggat ctgctcgatc accaggtgct taccccccgc atcgagcgac
      421 cactcgattg gccgcacgcc cggccgcgcg ctcggccacg gatagtcggc tcgcagacga
      481 ttaagcgggc atgtagcgtt cattggcgat gcgcggggcg gagtggttgt gccgtaagca
      541 cctcgctccg cgagatgcaa tggacggttt acgcgcggcg gcccttgcga atggagaatc
      601 ttccgctact cgcggagcga gtagcctgcg atgcggccga attcacgcag ggccgaatgc
      661 tagccgacgg cacgaagccg acctagagca atccctttgt gcttgcagag tgcattgcta
      721 tcccaggcga accaggtcat cgaccatctg ctgggcatgg gcggcagtgc ggacgaggcg
      781 atcaagccac agcgagccgg cggattgcct gccggccaaa aggtcgtcgg gcaagcaggc
      841 gaaaatcgcg gaggcgatct ggcggttgag atccgcgttg gggctgctgc cctgcagaat
      901 gccgcccagc gaggccgtca gcgaggcttg cacgagctgg caggtgaacg agcggtcggc
      961 gaacgttgtg gccgggtcaa gatcctcggc gaagtcgccg gcgcgggccg gatcgaaggc
     1021 aaattcggcc gtgcccggca gcgagctcag atgagccaaa agcatgtcgt tccagcgctc
     1081 cagccggcgg cggaggtggt tgatcttcgt cgcctgctcg ggtgtgaagg ccctgccgtc
     1141 ggcaacaagc tgcaagagtc ggcggcggac gtccagatgc ccgagccaca cgttgcgggc
     1201 gatgggcgag aggttttcgt cggcgtggtg ctcgtcgcag gcgacggcgg cagcggtcca
     1261 gatgcgggtc agcagctcgc tggccagaat ttcttcgcat gccgggccga tccgcggcca
     1321 ggcgagcgtc gcgggccgcg gaaactggcc cgccgcatcc gccagccgcc gcaacaggcg
     1381 cagccagcgg tcgatgcggc acttggaggc gacccagtac tgttcgatcg cggaatgagg
     1441 cacgcgctgg ccgctgcgga ccaggatcgg cccgtgaacg gccaccagag ccgccaattc
     1501 gacgagttcg cggacgtgca tgcgtggggc agattttcca acctgccgtt caagggttcg
     1561 aagcagacaa gaatgtctgc cccacttgtc aaaccgcgag cctctcccag cccgagggga
     1621 cgggggccgg gagaggcctc gcggctggcg ggacggaatt gcagtcgcca tgccgggcga
     1681 aatcgcgggg tgcggcctcg acgtaagtgc ttaagggcac aaaggttata gatcgggcag
     1741 ttataaatgg gcccagcgcg gcttgttgcc tttccgcacc agggccgtga cagcggtggc
     1801 gacttgtggc aacatcgcca cagccggcgg aaacaggagg cggccatgag aatcggagtc
     1861 atcagcgaca cgcacgggca tgtcaccaac acgctggcgg ctgtaaggct gctggaatcg
     1921 ctcgacgtcg aggcggttct gcactgcggc gacatcggca cgcccgagat tcctaagctc
     1981 ctcgccacgt ggcccacgca ctttgtgttt ggcaactgcg accacgagac gggcgagctt
     2041 aaagaggcca tttcggctgc cgggctgaca tgccacggcc gctttgccga cctggaactc
     2101 ggcggccgga agatcgccgt gctgcacagc gacgacgccc ggcgcttccg agaaacgatt
     2161 ttcagcggcc ggtacgatct ggtctgctac ggccacaccc atgaggccga atcgcaccgc
     2221 gaaggtcaga cgttggtact caaccccggg gccctctatc gagcccagcc acacaccctg
     2281 gcagtggtga atctgccgac gatggaggtc gaaatcgtca cagtggaata gcgagctcta
     2341 cccggccatc cgcgtcggct gggcctccgt ggcgctggcg gcggctgcgg cggagtagcg
     2401 aaactgcatc agatacgcgt cctccggcgt gtcttcatag aacgcgcgga gcacggagat
     2461 cgcccggaag ccggcggccc ggaaaaagag ctgggccgcg agattcgttt cgcgaatctc
     2521 gagcatgatc cggttgcgcc gctcgcgcga gagcttgctg gccagcttgc ggaccatttg
     2581 cgagccgacg cggcggcggc gaagctgctc gttgacggca aaattcagga tgtgcaggcg
     2641 gttcttgtgc agctcataga tcatgaagcc gacgacccgc tcgttgtgct cggcgaccat
     2701 gccgatgcag ttgcgctgcc gcaggcagcg gacgaagtcc tcttccgacc aggggaattc
     2761 gaagctttgg ctctcgatgg cgagtacctc gggcatgtcg cggcggatca tccagcgaat
     2821 gtgaaccgca agtggcttgg cgactggctt ggtcatagtc ttcatgccga cactccctct
     2881 aatcgcacca ccttgcactg ccgctcattc agtcggcagc cgtgccagat tgcgtgcggc
     2941 tcgatacgct gcctcttcgt cgaggaggac caatgaggtg tcatgtttcg gcacttgcgg
     3001 ttgccggtag gtgaaccgtc aggcgtccgc accgtcgggc tgatggctat ttcgattcgg
     3061 aaacgatcac ttcaccgcgc acatgcgctt gacaagatcg acggccgact tctggaggcg
     3121 gtctcgcgtt ggattaaccg ccgaccttgc cgccgaccat gccgccgact ctgccgccga
     3181 ctctgccgcc gaccatgccg ccgaccttgc cgccgaccat gccgccgacc atgccgccga
     3241 ctctgccgcc gaccatgccg ccgaccatgc cgccgaccat gccgccgacc atgccgccga
     3301 ctc
//
LOCUS       DASUGB010000628         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1876388, whole genome shotgun sequence.
ACCESSION   DASUGB010000628 DASUGB010000000
VERSION     DASUGB010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876388"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /gene="larA"
                     /locus_tag="VFV87_12015"
     CDS             <1..935
                     /gene="larA"
                     /locus_tag="VFV87_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX5104534.1"
                     /translation="IIEMVGSEIAATYRVENHHGEERDEHTYLGDSPRGVPIWIDSHY
                     VNADLKITTGLIEPHLMAGFSGGRKLICPGIAALETVKVWHGPDFLENPKADCGILEG
                     NPVHEENTWIGRHTGCDFIVNVVIDAERRPLKWVAGDMVAAFHEGVDFVRGVVRDTVK
                     EPVDIVVTSCAGYPLDTTFYQAVKGLTGALPIVKQGGTIVMCASLTEGIGSPQFQQLF
                     KENENLEIFVERILGKDYFVMDQWQLEELAKVRRKAKVKIVSNGLSADTLSGLFVEAA
                     PSIESAVAASLAEYGPAAKIAVIPKGPYVLAQVA"
     gene            complement(939..>2196)
                     /locus_tag="VFV87_12020"
     CDS             complement(939..>2196)
                     /locus_tag="VFV87_12020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5104535.1"
                     /translation="EGQVLLSSEKQMIGDFVPSGTDEATRDLFKYIALGAFLAGGALG
                     GVIFGAVADRIGRARTMVVTILMYSLFTCLTAACQEWWQVVVLRFLVALGVGGEWAVA
                     SAMVAEVFPPRARAWSGAIFHGSSVLGTYLAVAAGYFIVNNPAFGWRAAFVVGALPAL
                     LTLWIRWKLHEPERWTKDRQLAANRLTAAEQLRALFARGIAGKTAIGFGLAVVGLATF
                     WGVHIHGKELALRRARAEVERQAGLSADAPQAAREALWQQHDRELKPYEMWGMFLTTT
                     GGGLGLLSFGPLAEWLGRRRAFMLFHVGGLIVGLATFLTYRLWSDPVLYALLAVFGFW
                     TLGMHAGYAIYFPELFPTRLRSLGSGFCFNFARLTTAVMLVVNGYLAKVHVSLETAGS
                     ALCVLFLLGAAIAWLGPETKGTELAA"
BASE COUNT          447 a          733 c          676 g          340 t
ORIGIN      
        1 agatcatcga gatggtcggg agtgaaatcg cggcgaccta tcgggtcgaa aatcatcatg
       61 gcgaagaacg cgacgaacat acctatctcg gcgacagccc ccgcggcgtg ccgatctgga
      121 tcgacagcca ctatgtcaac gccgatctga aaatcaccac cggcctgatc gagccgcacc
      181 tgatggccgg cttctccggc ggccgcaagc tgatctgccc cggcattgcg gcgctggaaa
      241 cggtcaaggt ctggcacggg ccggatttcc tcgaaaatcc caaggccgat tgcgggatcc
      301 tggagggcaa ccccgttcac gaggaaaaca cctggatcgg ccggcatacg ggctgcgatt
      361 tcatcgtgaa tgtggtcatc gacgcggagc gaaggccgct caaatgggtc gccggcgaca
      421 tggtcgccgc gttccacgaa ggggtcgatt tcgtccgcgg cgtcgtccgc gacaccgtca
      481 aagagccggt cgatatcgtc gtcacgagct gtgccggcta tccgctcgat acgacgtttt
      541 atcaggcggt gaagggcctg accggcgcgc tgccgatcgt caagcaaggg ggcacgatcg
      601 tgatgtgcgc cagtctgacc gaagggatcg gcagcccaca gtttcagcag ctcttcaagg
      661 aaaatgagaa cctggagatt tttgtcgaac gaatcctcgg caaggattat ttcgtgatgg
      721 accagtggca gctcgaagag ctggcgaagg tccgccgcaa ggccaaggtg aaaatcgtca
      781 gcaatggcct ctccgccgac acgctcagcg gcctcttcgt cgaagccgct ccgtcgatcg
      841 aatcggccgt cgctgcttcg ctggccgaat atggccccgc cgcgaagatc gcggtcattc
      901 ccaaggggcc ctacgtcctg gcgcaagttg cctagttgct acgccgccag ttccgtcccc
      961 ttcgtttccg gccccagcca ggcgatcgcc gcgccgagaa ggaatagcac gcacagggcc
     1021 gaaccggccg tctccagcga gacgtgtact ttcgccagat agccgttgac gaccagcatg
     1081 acggctgtcg tcaaacgggc gaaattgaag caaaagcccg agccgaggct ccgcagtcga
     1141 gtcgggaaca gctcgggaaa atagatcgca tagccggcgt gcatgccgag cgtccagaat
     1201 ccaaagacgg ccagcagcgc gtagagcacc gggtcgctcc agaggcgata ggtcagaaac
     1261 gtggccagcc caacgatcaa gccgcccacg tgaaacagca tgaaggcccg ccgccgcccc
     1321 agccactcgg ctagcgggcc gaacgacagc agccccaatc cgccgccggt ggtcgtcagg
     1381 aacatgcccc acatttcgta gggcttcagt tcgcggtcgt gctgctgcca gagggcttcg
     1441 cgcgcggcct gcggcgcatc ggcagaaagt cccgcctggc gctcgacttc ggcacgtgcg
     1501 cggcgcagcg ccagttcctt gccgtgaatg tgaacgcccc agaatgtcgc cagtcccacg
     1561 accgccagtc cgaatccgat tgccgtcttt cctgcgattc cgcgagcaaa cagcgctcgc
     1621 aactgctcgg cggccgtcag ccggttcgcc gcgagctggc gatccttcgt ccaccgctcc
     1681 ggctcatgca atttccagcg aatccagagc gtgagcaggg ccggcagcgc gccgacgacg
     1741 aacgccgccc gccagccgaa cgcagggttg ttgacgatga aatatccggc ggccacggcc
     1801 aagtacgtcc ccaggacgct ggagccgtga aagatggccc ccgaccacgc gcgggcccgc
     1861 ggcggaaaga cttcggcgac catcgcgctg gccacggccc attcgccgcc cacgcccagg
     1921 gccaccagaa agcggagcac gacgacctgc caccactctt ggcacgccgc ggtaagacaa
     1981 gtgaacaacg aatacatcag gatcgtgacg accatcgtgc gggcccggcc gatgcggtcg
     2041 gcgacggcgc cgaagatcac gccgcccagg gcgccccccg ccaggaaggc gccgagcgcg
     2101 atgtacttga acagatcgcg cgtcgcttca tccgtcccgc tgggcacgaa gtcgccgatc
     2161 atctgcttct cgctcgacag cagaacctgc ccctcg
//
LOCUS       DASUGB010000629         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_626016, whole genome shotgun sequence.
ACCESSION   DASUGB010000629 DASUGB010000000
VERSION     DASUGB010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626016"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1434
                     /locus_tag="VFV87_12025"
     CDS             190..1434
                     /locus_tag="VFV87_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5104536.1"
                     /translation="MTAPSPNELAASLRKLDAGSPLYATEFVDLLLAAARAGGASDVH
                     LQPTVGRNEGPSYEVRWRIDGVLQLVGEFPRGSAADVVSRLKVLAGLLTYRADVPQEG
                     RIRSEQGQPGVEMRVSTFPTLHGERAVVRLFAGESQFHFPQDLGLPEEIEAALLRLLA
                     ETSGAILITGPAGSGKTTTAYACLREVVRTAEGRRSVVSLEDPIEVAIAGVSQSQVHP
                     ASGFDLATGLRSLMRQDPEVILVGEIRDRPTAETVFQAALTGHLILSTFHAGGAAGGI
                     SRLIDMGIEPYLLRSGVRAMLHQRLVRRLCECAMPAADGDSFLGLPLVRAQESAGCEA
                     CRHTGYRGRLVLAELLPPLVGELGEAVLSRRDALELARLATAAGMTTLFQRACTAIES
                     GVTDPAEVRRVLGFAEAAGELA"
     gene            1561..2640
                     /locus_tag="VFV87_12030"
     CDS             1561..2640
                     /locus_tag="VFV87_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5104537.1"
                     /translation="MELVTITASLASQPVTLDQLAALSDEMAALARAGVPLDRGLREL
                     ARDMPGRLGKLADQMGQRLAAGQPLDKAVQELSTTLPPAYRAVLTAGQRSGKLSAALE
                     GVAHSARHISQLRQSIGLAVIYPLIVLLVAWGLLVFVLTQIAPVMLQVMQQFELKTDP
                     WDRLVGGLSGSALWWGTLIPAAALVWLAWIWYRSGRVAQGVELHPWLGLGAIPTLVRM
                     QRAGRTASLAELLALLVSNHVPLPEAIELASAAVGSPQMERGGKELAERLRRGEKMDR
                     IPAGFPPLVAWTLVSGSDASRLPHVLSRTAQTYREEVARRGQWLQLYVPLVMIVGICG
                     TVAMLYALLTLLPWILVMYRLAEPV"
     gene            2664..>3094
                     /locus_tag="VFV87_12035"
     CDS             2664..>3094
                     /locus_tag="VFV87_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5104538.1"
                     /translation="MSDHTAAADSPLSAAGAEVVLTRAGQLASAGMPLPAGLRAAADE
                     ADSPRVARGLRRLAAEVERGRPLETIVAELRLPAHLKGLLQAAQRTGHFGAVLAEWME
                     NRRAARLHWRSIEAAVAYPLIVLGMTIAVYVLFLVFLVRPFR"
BASE COUNT          460 a         1023 c         1094 g          517 t
ORIGIN      
        1 tgcggcggac gttgtccaga tggcgaactg ttgccgcaat tgactcccga ccccgtaggc
       61 tgctagcagg cgcgcaggtc gaagcgcgag cgcagcggcg ctataggtat tcttcggatg
      121 gcagactcta gcgattgagc aatcgttaac gaacgtagcg gaatgagaag ctgggcaagt
      181 aagtgacgca tgacagcacc ctctccaaat gagctcgccg cgagcctgcg caagctggat
      241 gctggcagcc cgctgtatgc gacggagttc gtggacctgc tgctggcggc cgcacgcgcc
      301 ggcggggcga gcgatgtgca cttgcagccg acggtcggac ggaacgaagg tccctcctac
      361 gaggttcgct ggcgaatcga cggcgtgctg caactcgtcg gcgaattccc gcgcggctcc
      421 gcggcggatg tggtgtcgcg gctgaaggtg ctggcaggac tgctcacgta tcgcgccgat
      481 gtgccgcagg aagggcggat tcgcagcgag cagggacaac ccggcgtcga gatgcgcgtc
      541 agcacgtttc cgacgctcca cggcgaacgg gcggtggtgc ggctctttgc gggcgaatcg
      601 cagtttcact ttccgcagga tctgggactg ccggaggaga tcgaagcggc gctgctgcgg
      661 ctgctcgccg aaacgagcgg ggcgatcctg atcaccggac ctgccggcag cggaaagacg
      721 acgacggcgt atgcgtgcct gcgggaagtg gtgcggaccg ccgaaggccg gcgaagcgtc
      781 gtgtcgctcg aggatccgat cgaagttgcg atcgccggtg tgagccagtc gcaagtacac
      841 ccggccagcg gcttcgatct ggcgaccggg ctgcggtcgc tcatgcggca ggacccggag
      901 gtaatcctgg tgggcgaaat ccgcgaccgg ccgaccgccg agacggtctt tcaggcggcc
      961 ctcacggggc atctcatcct cagcacgttc cacgccggcg gcgcggccgg cggcatcagc
     1021 cggctgatcg acatggggat cgagccgtac ctgctgcgca gcggcgtacg ggcgatgctg
     1081 caccagcggc tggtgcggcg cttgtgcgaa tgtgcgatgc ccgccgcgga cggcgattcg
     1141 tttctcggct tgccgctggt gcgggcgcag gaatcagccg gctgcgaagc ctgccgtcac
     1201 accggctatc gcggccgact ggtgctcgcc gaactgctgc cgccgctcgt gggcgagctg
     1261 ggcgaagcgg tcctcagccg ccgcgacgcg ctggagctgg cccgcctcgc cacggccgcc
     1321 ggcatgacga cgctcttcca gcgcgcctgc accgcgatcg aatccggcgt caccgaccct
     1381 gccgaagtcc gccgcgtgct cggcttcgcg gaagcggcgg gcgaactagc gtgatacagt
     1441 agggtgggct atgcccaccg tcgtggcaga aggcaaccat tactcgatga gcatcgtggg
     1501 cacagcgagc atcgtgggca cagccagcat ggtgggcaca gcccacccta caagttcgag
     1561 gtggaactcg tgacgatcac cgcttcactc gccagccagc cggttacgct tgatcagctt
     1621 gccgcgctct ccgacgagat ggcggcgctg gcccgcgcgg gcgtgccgct cgatcgcgga
     1681 ttgcgggagc tggcccgtga catgcccggc cggctgggga agcttgccga tcagatgggc
     1741 cagcgcctcg ccgccggcca gccgctcgac aaagcggtcc aagagctgag caccacgctg
     1801 ccgccggctt atcgcgcggt gctgaccgcg ggtcagcgca gcggcaagct ctccgcggct
     1861 ctcgaaggcg tcgcccactc cgcccgccac atcagccaac tgcggcaatc gattggcctg
     1921 gccgtcattt atccgctgat tgtgctgttg gtcgcgtggg gattgctcgt gttcgtgcta
     1981 acgcagatcg cgcccgtcat gctgcaggtc atgcagcagt ttgagctgaa gacggacccg
     2041 tgggaccgtt tggtcggcgg actaagcggt tcggccctgt ggtggggaac cctgattccg
     2101 gccgctgcgc tggtgtggct ggcctggatt tggtatcgct cgggccgggt agcgcaaggg
     2161 gttgagctgc acccgtggct gggcctcggc gcgattccga cgctggtccg catgcagcgc
     2221 gcgggccgca cggcttcact ggccgagttg ctggcgctcc tcgtcagcaa tcacgtgccg
     2281 ctgccggagg cgatcgagct ggcctcggct gccgtcggat cgccacagat ggagcggggg
     2341 gggaaagaac tggcagagcg cctccgtcgc ggcgagaaaa tggaccgcat ccccgcggga
     2401 ttcccgccgc tggtcgcgtg gacgctggtc agcggcagcg acgcgtcccg cctgccgcac
     2461 gtcctctcgc gcaccgccca gacctatcgc gaggaagtcg cccgccgcgg gcagtggttg
     2521 cagctctatg tgccgctggt gatgatcgtc ggcatctgtg gcacggtcgc gatgctctac
     2581 gccctgctga cgctgctccc ctggattctc gtcatgtacc gcctggccga gccggtttag
     2641 cctgggccga ccgtcaatcc cccatgtccg atcacaccgc cgctgccgat tcgccgcttt
     2701 ccgccgccgg ggccgaagtc gtgctgacgc gcgccggcca gctcgctagc gccggcatgc
     2761 cgctgccggc gggcttgcga gccgctgccg acgaggccga ttcgccccgc gtcgcccgcg
     2821 gcctgcggcg actggcggcg gaagtcgagc gcggccgccc gctggagacc atcgtcgccg
     2881 agctgcgcct cccggcgcac ctgaaaggct tgctgcaagc ggcccagcgg acgggccact
     2941 tcggagcggt cctcgccgag tggatggaaa accgccgcgc cgcccggctg cactggcggt
     3001 ccatcgaggc agccgtcgcc tatccgctga tcgtgctggg aatgacgatt gccgtttatg
     3061 tgctgtttct cgtgttcctg gtgcggccgt tccg
//
LOCUS       DASUGB010000630         3950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_904937, whole genome shotgun sequence.
ACCESSION   DASUGB010000630 DASUGB010000000
VERSION     DASUGB010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904937"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFV87_12040"
     CDS             complement(<1..373)
                     /locus_tag="VFV87_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104539.1"
                     /translation="MSSLLLVAALALSADPQPQVLDDRLQIQLVSEHPDVRTPTGIAV
                     DEKGRVLVIESHTHFPPPNYDGPKHDRILLLDDFDPATGKARQISTFFEGTTHTMAIA
                     LYHDGSFYVATRMEIFRLRDKD"
     gene            complement(529..1722)
                     /locus_tag="VFV87_12045"
     CDS             complement(529..1722)
                     /locus_tag="VFV87_12045"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5104540.1"
                     /translation="MRILVGWDSAEELETIQSFLNIGENSAAVFGDAAAFAAVFESET
                     FDVILQALSYPSEVESFALFQRLRRANPDTPVVGAWRAGDINLLAKFMMSGLHSSILR
                     DPAGEYIMLLTTCLEAAHQAVQARRAQILAEKLREEVESVRQLQESVIPNNLPQLRDY
                     KVVARYEPSQIHVLGDRPVVMAGGDYYDAFRLRDDQLVMMLGDAAGHGVKACMSIMTM
                     HTLIGMIRSQTYWDTAEFVNDVNRRLCKSSIVAGDQGGFITLLYCSLDLHKHTLQWTS
                     AGHPIPLLQDLSTNEVRAVGTADQAGLPLVVSEDWTYPSWQVELPPRCRVVLYTDGLE
                     EAFPSDDERHQQFGVQGIIGTLRETADQPLESALDELFAASHRATKGAGRLDDTSIML
                     LERGA"
     gene            complement(1928..1998)
                     /locus_tag="VFV87_12050"
     tRNA            complement(1928..1998)
                     /locus_tag="VFV87_12050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1964..1966),aa:Gln,seq:ttg)
     gene            complement(2128..2799)
                     /locus_tag="VFV87_12055"
     CDS             complement(2128..2799)
                     /locus_tag="VFV87_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5104541.1"
                     /translation="MSDAKDKPDETNFDGPVPKAVVSRLSLYLRELQHQVRDGQETTS
                     STQLGKLLGFTDAQVRKDLAYFGQFGYPGIGYRCSELIAAIKGILGTSQPWPLAIVGL
                     GNLGRALMGYKGFQQQGFQIVAAFDADPAKVGKPVEGLQVHHMDDVGRIAAERNIRLA
                     IVAVPAPSAQPVADALVAAGIEGILNFAPVTLALPKNVRVVGVDLAIELEQLSFAVVN
                     QLSKS"
     gene            complement(2982..3431)
                     /locus_tag="VFV87_12060"
     CDS             complement(2982..3431)
                     /locus_tag="VFV87_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5104542.1"
                     /translation="MKRNSHKSAFTLVEVLIVVVIMAILAATIIPQFTDSTNDAKVST
                     TKFNLHTLRSQIELYRAQHNGLVPTGTLVELTKSTDIDGNQGTGTSFPYGPYIREIPM
                     NPFTNSQTVTVITADPAAAGDVTGSGGWLYNDTTGGIWIDHADHFGE"
BASE COUNT          789 a         1179 c         1216 g          766 t
ORIGIN      
        1 cgtccttgtc gcggaggcgg aagatttcca ttcgcgtggc gacgtagaac gagccatcgt
       61 gatagagggc gatcgccatc gtgtgcgtcg tcccctcgaa gaacgtgctg atctggcggg
      121 ccttgccggt ggccggatcg aaatcgtcca gcaagagaat acggtcgtgc ttcggcccgt
      181 catagttcgg cggcgggaag tgcgtgtggc tctcgatcac cagcacgcgg cccttttcat
      241 cgacggcaat gcccgtcggc gtccgcacgt cgggatgctc cgagacaagc tggatctgca
      301 ggcggtcgtc cagcacttgc ggctgcgggt cggcggagag ggcgagggcg gcaaccagca
      361 gaagagaaga catggcgata gcgcagggcg gagggggcta agtggaattg gagacacttc
      421 cccattgtac tgagtactaa gtactcaata ctcggctacg aagcctcacc ctgattcgcg
      481 actcgtgcgg gcactcgtat cggtcctgcc gcggagcggc ggactactct aagcgccccg
      541 ttccagcagc atgatcgacg tgtcgtcgag gcgaccggcg cccttggtgg cgcggtggga
      601 ggcggcgaag agctcgtcga gggcagactc caatggctgg tcggccgttt cacgaagcgt
      661 gccgatgatc ccttggactc cgaactgttg gtgccgctcg tcatcgctcg gaaaggcctc
      721 ttccagaccg tcggtataga gcacgactcg gcagcggggg ggcaattcaa cctgccacga
      781 cggataggtc caatcttcgg acactaccag cggcaagccg gcctgatcgg ctgtgccgac
      841 tgcgcgaacc tcgttcgtcg acagatcctg caagagcggg atgggatggc cggcgctcgt
      901 ccactggagc gtgtgcttgt gcagatccag gctgcaatag agcagcgtga tgaatccgcc
      961 ctggtcaccg gcgacgatcg agctcttgca caagcggcgg ttgacgtcgt tgacaaactc
     1021 ggccgtgtcc caataggtct ggctgcggat catgccgatc agcgtgtgca tcgtcatgat
     1081 cgacatgcag gccttgacgc cgtggccggc cgcgtctccc agcatcatga caagctgatc
     1141 gtcgcgcagg cggaaagcgt cgtagtagtc gccgcccgcc atgacaaccg ggcggtcgcc
     1201 gagtacatga atttgcgaag gctcgtaacg ggcgacgacc ttgtaatcgc gaagctgcgg
     1261 caggttgttg ggaatcaccg attcctgcag ttgccggacc gactcgactt cttcgcgcag
     1321 tttttcggcc aggatctggg cgcgtcgcgc ctggacggcc tggtgagcgg cttccaggca
     1381 ggtggtgaga agcatgatgt actcgccggc tggatcgcga aggatcgacg agtgcagccc
     1441 ggacatcatg aacttcgcga gcaggttgat atcgccggcc cgccaggcgc cgacaaccgg
     1501 cgtatcggga ttggcccttc gcagccgctg aaatagggcg aacgattcga cttccgaggg
     1561 atagctaagc gcctgcagga tgacgtcaaa cgtctcgctt tcgaagaccg cggcaaacgc
     1621 tgcggcgtcg ccaaatacgg ctgcggagtt ctcaccgata ttgagaaacg actggatcgt
     1681 ttccagctct tccgcactgt cccagcccac gagaatgcgc ataccgactc ccaagcaaag
     1741 tgccacccga cgaatccgga ctagcgcctc tgccgcgagg gttggctcgc gctcaggccc
     1801 gcgaaatgcg aacgcttctt gttccaagaa gtttggatga attcccgtcc tggattattg
     1861 ccacccgctc caggaggttt caagcaaaac caggctgcgg catcccgcaa atccacaacc
     1921 tagggagttc ggggactagg attcgaacct agacaagcag aaccaaaatc tgctgtgcta
     1981 ccgttacacc atccccgaat ggttgacgag gcattgtaag cgccggctcg cgcctggcaa
     2041 tcagagggcg gccgtcgctt ggcggccaga gccctctcgt aacgccatgc tcgccaacaa
     2101 cttgtggcgt ttcccaagaa cagagactca gctcttgctg agctggttca cgacggcaaa
     2161 tgagagttgc tccagttcga tcgccagatc aacgccaacc acccggacgt tcttgggcaa
     2221 ggccagggtg accggcgcga aattaagaat gccctcaatc ccggccgcga ccagagcatc
     2281 ggccaccggc tgcgcggacg gagcggggac agctacgatc gccaggcgaa tattgcgttc
     2341 ggcggcgatg cgacccacgt cgtccatgtg gtgaacctgg agcccctcga cgggcttacc
     2401 caccttggcc gggtcggcat cgaacgcggc gacgatctgg aatccctgct gctgaaagcc
     2461 tttgtagccc atcagggccc ggccgaggtt tcccaggccg acaattgcca agggccaagg
     2521 ctgactcgta cccaggatgc ccttgatggc cgcaatcagc tcgctacagc gatacccaat
     2581 gcccggatac ccaaactggc cgaaataggc caaatccttc cgcacctgtg catccgtaaa
     2641 tcctaatagc ttcccgagtt gcgtcgagct ggtcgtttcc tggccgtcgc ggacctggtg
     2701 ctgcaattcg cgcaggtaaa ggctcaacct gctgacgact gccttgggca ctgggccatc
     2761 gaaattggtc tcgtccggct tgtccttggc atcgctcatg gctgtagtgc ccccggcgat
     2821 ggcctgggca agcaacctac tgtaaaggct cattgtcgga gccacaaggc aatcgggctc
     2881 caggcatgat ctgcgtgcgc atgaaatcgg ccctgacttc ccaggggggc cagggccgcg
     2941 cttgttggat tcaatcggaa cgacgacgtt tgacgccgat gctattcgcc gaagtgatcc
     3001 gcgtggtcga tccagatgcc gcccgtcgtg tcgttataca gccagccgcc cgaccccgtg
     3061 acatctcccg cggcagccgg atcggcggtg atcaccgtca ccgtctggct gttggtgaag
     3121 ggattcatcg gaatttcgcg gatatacggg ccgtagggaa agctcgtgcc ggtcccctga
     3181 ttgccgtcga tatcggtgct cttcgtgagt tcgacgagtg tgccagtcgg caccagcccg
     3241 ttgtgctgcg cccggtagag ctcgatctgc gatcgaagcg tgtggagatt gaacttggtg
     3301 gtgctgacct tggcgtcgtt ggtgctgtcg gtgaactgcg gaatgatcgt ggccgcgaga
     3361 atggccataa tcaccacgac gatgagcacc tcgaccaggg tgaaagctga tttgtgtgaa
     3421 ttgcgtttca tggattgttg ccttaacgac ctggggatgg tggtcagcgc ctcaaaggcc
     3481 cgttcagtcg cgcgaccgac cgcggcagcc gcctgcaaca agggccgggg agcaaccgcg
     3541 atgaaacact gcgcctctaa gtcgccgatt cgtcgctgaa tcgggtcgct agaagtacct
     3601 aggtcatagc gggaaagcgg tcaagaaatt cttgcgctaa cgtgatttac cgcatggcgg
     3661 tgcagcgtca tttgccccgc gactgacgaa tccgacaagc cggccgcccc gctaagctgc
     3721 acgtgcccta atggcggcag gtcgggcaaa atgcggattg acgcttcaat cgttcagaag
     3781 cgcacgaaca ggggatcagt cgcagcccta actcgcgccg caggaagact ttgcgcagaa
     3841 cactcggact tcctggaaga aatttcttgc gcctgactaa tccattctcc atttacccca
     3901 tttcaagcaa ccgacccagt ttgacgatgt accactacat ccggtgtctt
//
LOCUS       DASUGB010000631         7456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1601002, whole genome shotgun sequence.
ACCESSION   DASUGB010000631 DASUGB010000000
VERSION     DASUGB010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601002"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFV87_12065"
     CDS             <1..500
                     /locus_tag="VFV87_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEX5104543.1"
                     /translation="EGTPVAVVSLLGRVFMRPVDCPFAAADRVLAEIPADVKVRIVDF
                     HAEATSDKQLMGRYLDGRVTAILGTHTHVTTADEQLFPGGTAFQCDVGMTGPHHSILG
                     RRIDRVLETTVTFQPTLFDVAEGDVRLCGTIVEADPATGRATAIRRLVLTEAEADNMA
                     SSPDS"
     gene            709..1020
                     /locus_tag="VFV87_12070"
     CDS             709..1020
                     /locus_tag="VFV87_12070"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX5104544.1"
                     /translation="MGTHKQLGLPDCTIVQWFGMRCPSCGMTTSWSHMMRGHVISSFQ
                     ANAGGALLALTALACGPWLIVSGCRGRWTCTQPHEGTTLLIGVTIIVVTLADWTLRVW
                     I"
     gene            1120..1788
                     /locus_tag="VFV87_12075"
     CDS             1120..1788
                     /locus_tag="VFV87_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104545.1"
                     /translation="MQRSLTLVGHGTIALFAGCILLACTGCGSHLGLIDYWRRGILPV
                     DAKFPGLEGKRVAVVCLDGNSLHGPGSEADTIARAVTTTLGAKVADIRIVRQSEIADW
                     IDSQNEDLSDYRDVGRGVKADMVVGIDLISFNLHEGQTLLKGRAKVGVKVFDMTKGGQ
                     VVYESPPKEITWPESGARHVTENEDNFRNVFIYTILADKIARDFYAYEAISDVAIDAQ
                     SLAN"
     gene            complement(1903..3906)
                     /locus_tag="VFV87_12080"
     CDS             complement(1903..3906)
                     /locus_tag="VFV87_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5104546.1"
                     /translation="MRIIQGLRQWWQRQTGEEETPFDGDSPAFIVSLLVNLAIVVSLG
                     LIPLMIQDNQVTLTIDATAPEEIEEMKIPEEFVFSEQPAEEVGANSVAGEAVALSMAP
                     VISEISQIPNHLDVETTVDNAQIEINNAIEVATGLHYNQNLAVKGAAGVGTTGAEGAI
                     DRLTHEILLSLEERKTLVVWLFDGTASLIPQRNAIHDRFDRIYKELGIIEAAGDESFT
                     KHEDKPLLSSIVAFGSGMQLMTKEPTDDLAELKAAVKNIPNDESGMENVFSAIHEAAR
                     HYADFRYTTAEKPNPDRNVMLVVFTDEAGTDANKAEETIKMCRRWAMPVYVVGVPAPF
                     GRRETQMKWVDPDPKFDQTAQWGVVEQGPESYLPERIKLAFSGSKEDDEPIDSGFGPF
                     ALTRLCYETGGIYFAVHPNRNVTRAVSRGETAAFSSYVSHFFDPETMRKYRPDYVSVT
                     EYQKRASQNKARWALVQAAQASQLTQMDSPTLRFVKSDEAAFANALSEAQQAAAALEP
                     KINSLYEILRQGEADREKETVLRWQAGYDLAIGRALAVKVRTETYNAMLAAAKRGLKF
                     KDEKNNTWILKPSDEISVGSQYAKLGERAKMYLNRLATDHPGTPWAMLAERELKDPLG
                     WSWEEEFTDTSPRRMGDGNGNPNPNDARRMMTKPPPKRPPPKL"
     gene            complement(4368..5009)
                     /locus_tag="VFV87_12085"
                     /pseudo
     CDS             complement(4368..5009)
                     /locus_tag="VFV87_12085"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
     gene            5101..6600
                     /locus_tag="VFV87_12090"
     CDS             5101..6600
                     /locus_tag="VFV87_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX5104547.1"
                     /translation="MTGYRWLPLWISLALSTSSAESGLAQEAAPHEPAGMRSAAAAEH
                     LSAEGNHANLGARLPLWTVTPFVALLLAIAVLPLVVEHWWHHNHNKAIVAGLLASLIG
                     IYLVAAWGATGWHEVWDKLKEYASFMILLGALYVISGGIYIKGSLSGTPLANTGLMAI
                     GAALASVIGTTGASVLLIRPLLRANTSRNAKAHVVIFFIFIVSNCGGLLTPLGDPPLF
                     LGFLKGVPFLWTLENLWPHWLVVNAVLLATFHIWDQVVFAREERAREGSQLEEVLKHE
                     PLGISGWPNFLFLGGVVATIYCSGQYGWPWGAAELLMAVLAVAAYVTTPADNRKANQF
                     TFGPLVEVAVLFIGIFVTMAPALQILNAWGKGERDILGLAFGLSESWHFFWATGVLSS
                     FLDNAPTYLTLAATAAGKSGVAVEGSAYLRTFLDQGGADSAGLLAAISCGAVFMGANT
                     YIGNGPNFMVKAIAEENGVKMPGFFGYMAYSGAILIPIFIAVTLIFFRS"
     gene            6638..>7456
                     /locus_tag="VFV87_12095"
     CDS             6638..>7456
                     /locus_tag="VFV87_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5104548.1"
                     /translation="MTMLEKLLLPEIRELIHDKDLPTLREVLNRWLPADIADLLGDLG
                     PYEDIVAFQCLEVELAAKTFAHLQRTTQEALVETLPEPELARILNQLAPDDRTALLEP
                     LPHQEVERLLTLLTPENQRVARSLLSYGRGTVGRLMTPDFIGVREAWNVSLVLDHVRR
                     VGKDSETLNAVYVYDDEHHLVDDIRMREILLVAPETTVRSLMDRRYVALNVTDAEATA
                     VEMFRKYDRTVLPVIDRRGVLLGVVTIDDVLDVAEESATREIQKFGGLEALEEPY"
BASE COUNT         1283 a         2264 c         2389 g         1520 t
ORIGIN      
        1 gagaggggac gcccgtcgcg gtcgtcagtt tgctcggccg cgtgttcatg cggcccgtcg
       61 attgcccctt cgcggcagcg gaccgcgtgc tggccgagat acctgcggac gtgaaggtcc
      121 ggatcgtcga tttccacgcc gaagcgacca gcgacaagca gctcatgggc cgctatctcg
      181 acggccgcgt gaccgccatc ctcggcacgc atacgcacgt caccaccgcc gacgagcagc
      241 tctttccggg cggaaccgcc tttcagtgcg acgtcggcat gaccggcccg caccacagca
      301 tcctgggccg gcggatcgac cgcgtcctgg aaacgaccgt cacctttcag ccgacgctgt
      361 tcgacgtcgc cgagggggac gtgcgactgt gcggaacgat cgtcgaagcc gatccggcca
      421 cgggcagggc gaccgccata cgccggctgg tcctcaccga agccgaggcg gataatatgg
      481 cttcgtcccc tgactcctga cccctgtccc ttggccccct cgcttcttgc tcttgcgaga
      541 cccaaccctg tgagctattc ttcgcccccc cttgctgccg gtcccaaccg cgggcctttt
      601 gcgcctgcgc gaatcggttg gctgcaacgg ggggcgatgg tgctggcggg aaccttgtta
      661 gtggggttgc tcgtgaccgc ggctgtgctc acgcccagtc cgcgcgggat gggcactcac
      721 aagcagctcg gcttgcccga ttgcacgatt gtgcaatggt tcgggatgcg ttgcccgtcg
      781 tgcggaatga cgacctcctg gtctcacatg atgcgaggcc acgtgatctc gtcattccag
      841 gcaaatgccg gcggggcctt gctggcactg acggcactgg cctgcggtcc gtggctgatc
      901 gtttccggct gtcgcggccg ttggacgtgc acgcagccgc atgagggcac gacgctgctg
      961 atcggagtga ccatcatcgt ggtgactttg gccgattgga cgctgcgggt ttggatctga
     1021 agggtcgcac atgacgaagc acggcttcgt cctacgtagg tcgaagccgt gcttcggccg
     1081 gctggaacgg attccggcat cgcacgcaaa ggattgccca tgcaacgctc gctcactctt
     1141 gtcggccacg gaacgattgc tcttttcgcg ggctgcatcc tcctggcatg caccggctgc
     1201 gggtcgcact tgggcctgat cgattactgg cggaggggca tcttgcccgt cgatgccaaa
     1261 ttccccgggt tggaaggcaa acgcgtcgcc gtcgtctgcc tggacggaaa ctcgctccac
     1321 ggccccggca gcgaagccga caccattgcc agggcggtga cgacgacgct cggagccaag
     1381 gtagccgaca tccgcatcgt gcggcagtcg gaaattgccg actggatcga tagccagaat
     1441 gaggacctgt ccgactatcg cgacgtgggc cgcggcgtaa aagccgacat ggtcgtgggt
     1501 atcgacctga tcagcttcaa cctgcacgag gggcagacgc tgctgaaggg ccgggccaag
     1561 gtgggcgtca aagtgttcga catgaccaaa gggggccaag tcgtttatga atcgccgccg
     1621 aaggagatta cgtggccaga gagcggcgcg cggcacgtga cggagaacga ggataacttc
     1681 cgcaacgtgt ttatctacac gattctggcc gacaagatcg cccgcgactt ctacgcctat
     1741 gaggcgatca gcgacgtggc gatcgacgcc cagagcctgg cgaactagcg ctttggagcg
     1801 cggcgactcg tcgccgcttt tagacttttg ggaatgagcc gtgacggaac tgcgctttcg
     1861 gcggcgatcc caaagcggtg atgtatcacc gcactccata tcctatagct tcggcggcgg
     1921 gcgcttcggc ggcggcttgg tcatcatccg ccgggcgtcg tttggattgg ggttgccgtt
     1981 gccgtcgccc atccggcgag gcgaagtatc ggtgaactcc tcttcccacg accagccgag
     2041 cgggtccttc agctcgcgct cggccagcat agcccagggc gtgcccgggt ggtcggtcgc
     2101 caggcgattg agatacatct tggcccgctc gcccagcttc gcatactggc tgccgacgct
     2161 gatctcgtcc gacggcttga ggatccaggt gttgttcttc tcgtccttga acttcagccc
     2221 gcgcttagcg gcggcgagca tcgcattgta agtctcggtg cggactttga cggccaaggc
     2281 ccggccgatc gccaggtcat agcccgcctg ccagcgaagg accgtttcct tctcacgatc
     2341 ggcttcgccc tggcgaagga tctcgtacag cgaattgatc ttcggctcca gtgcggccgc
     2401 ggcttgttgg gcctcggaaa gagcgttggc gaaggccgct tcgtcggact tcacgaaccg
     2461 cagcgtgggg ctgtccatct gtgtgagctg cgaggcctgc gccgcctgaa ccagcgccca
     2521 gcgggccttg ttctggctgg cccgcttttg atattcggtc accgagacgt agtcgggccg
     2581 atatttccgc attgtttccg gatcgaagaa gtgcgagacg tatgacgaga acgccgcggt
     2641 ctcgccgcgt gataccgccc gcgtcacgtt ccggttggga tgcacggcga agtagatgcc
     2701 gcctgtttcg tagcacagcc gcgtgagggc gaacggtccg aagcccgaat cgatcggctc
     2761 gtcgtcttcc ttggagccgg agaaggcgag cttgatccgc tcgggtagat acgattcggg
     2821 tccctgctcg accacgcccc actgcgcggt ctggtcgaat ttcgggtcgg gatcgaccca
     2881 cttcatttgc gtttcgcggc ggccaaaggg ggccggcacg ccgacgacat acaccggcat
     2941 cgcccagcgg cggcacatct tgatcgtttc ttcggccttg ttcgcgtcgg tccccgcctc
     3001 atcggtgaag acaacgagca tcacgttccg atctggattc ggcttctcgg cggtagtata
     3061 gcggaaatcg gcgtagtgcc gggcggcctc atgaatggct gaaaagacgt tctccatccc
     3121 cgattcatcg ttgggaatgt tcttcacggc ggctttgagc tcggccagat cgtccgtcgg
     3181 ctccttcgtc atcaattgca tgccgctgcc gaaggcgaca atcgacgaga gcagcggctt
     3241 gtcctcgtgc ttggtgaacg attcgtcgcc agcggcctcg atgatgccga gctccttgta
     3301 gatccggtcg aatcgatcgt ggatcgcgtt ccgctgggga atcaggctgg cggtgccgtc
     3361 gaacaaccac acaaccaatg tttttcgttc ttccagcgag agcagaatct catgagtcag
     3421 gcggtcgatc gccccttccg cgccggtcgt gccgacgccc gcggccccct tcacggccag
     3481 gttctggttg tagtgtaggc cggtcgccac ttcgatcgcg ttattgatct cgatctgggc
     3541 gttatcgacc gtggtttcta cgtccaggtg attgggaatc tgcgaaatct cagaaatcac
     3601 cggggccatc gagagggcga ccgcctcacc cgccacgcta ttggccccca cttcctcggc
     3661 cggctgctcg ctgaagacga actcctccgg gatcttcatc tcctcgattt cttccggcgc
     3721 cgtagcatcg atcgtcagcg taacctgatt atcctgaatc atcagcggga tcaggcccag
     3781 cgaaaccacg atcgccaggt tcaccagcag gctgacaatg aaggcgggcg aatcgccatc
     3841 aaatggcgtc tcttcctcgc ccgtctgccg ctgccaccac tgccgcaatc cctggatgat
     3901 tctcacgggt gctctcctgg cgcctctggt acagttccac gtgcagaccc cgaccgcctg
     3961 gaccggctct cctgcgaatg gttcaccggt tcgcactacc taactcgttt cgcctgactg
     4021 gtttgcaaat cttgagtcaa gctcgcgcga cccattttca gcgattgtcg gcgcaaatgc
     4081 aagctttttg gcctcccgac ttgccttact tctaaacgac ttatggccgc caaagtaccc
     4141 aaaacgggcc tcgccagctt cattttgcca ttccccgaca aatctattca aattccttca
     4201 accaacacct catacctgga tggcttgccc tgccacaatc tatgtcgaac atggctgttt
     4261 ttcgctccat cttcgttaat ggccgaccac gatggaaccg acaagatcgg ggggggtaat
     4321 gagcagtcaa aacatggatt ttaggggtga gccagggccg tggcaatcta gcgcttcagg
     4381 atcttgaagg cgcagttcga gccccagcct tgcttgaagg ccaggtggat atagagcatg
     4441 gccagcggtt cgagatagcg ggcgctggcg agcgggccgg catcgaccgg gtcgaagccg
     4501 aggtcggcga taagctggcg gacggattct ttggcggcgg cgtcgtcgcc gcaatagaac
     4561 atggctgccg gatagtcgcc gaactgcgga ttcgacatca ctttggtgct ggccgtgttg
     4621 aaggctttga cgacccggct gccctgggcc caggtggcga tgtgctcggc ggcggaagtc
     4681 gtgtggccga gctcaatgcc agtgaaatcg gcggtgagcg ggttggtgca atcgatgagg
     4741 atgcggccgg cgagatcgcc caggccggtg agggtctcct gcgctgcggg ccagggcacg
     4801 gcaaggacga tcacgtcggc gccggcgatg gattccttca ggggaaccgc cgtggcgcct
     4861 tggccgcagc gctgcagaag ttcgaggacc ttttcgctct ccggcaggcg ggagccaaag
     4921 gtgatgccat gaccggcttt agcccagcgt gcgccgagga ccgaaccgac tccgccggta
     4981 cccaagatgg cgatctgcat ggctctcccc tttcggtcag gaagcgatca tcgtaccctt
     5041 tcttggcctc tgggcgaggg cggggccgag gttggccaca ggcgcacggg agaacacgcg
     5101 ttgaccggat atcgctggct gccgctctgg atttcgctcg ccctcagcac ttcgtcagcc
     5161 gaaagcggac tagcgcagga ggccgcgccg cacgagccgg ccgggatgcg gtcggcggcg
     5221 gcagcggaac atctgtccgc ggagggcaat catgccaact taggcgccag gctgccgctc
     5281 tggacggtga cgccgtttgt cgcgctattg ctggcgattg ccgtgctgcc gctcgtggtc
     5341 gagcactggt ggcaccacaa ccacaacaag gcgatcgtcg cggggctgct cgcgtcgcta
     5401 atcggcattt atctggtggc ggcctgggga gcaaccggct ggcacgaggt ctgggacaag
     5461 ttgaaagaat acgcgtcgtt catgatcttg ctcggcgcgc tgtacgtgat cagtggcgga
     5521 atttacatca agggctcgct gtcggggacg ccgctggcaa acacgggact gatggcaatc
     5581 ggcgcggcgc tggccagcgt catcggcacg acgggcgcct cggtcctgct gattcggccg
     5641 ctcctcaggg cgaacacttc gcgcaacgcg aaggcgcacg tggttatttt tttcatcttt
     5701 atcgtgagca actgcggcgg attgctcaca ccgctgggcg atccgccgct gttcctgggc
     5761 tttttgaaag gcgtgccgtt cctgtggacg ctcgagaact tgtggccgca ctggctcgtc
     5821 gtcaatgcgg tgctcttggc gacgttccac atctgggatc aggtagtctt tgctcgcgag
     5881 gaacgggcgc gcgagggctc gcagctcgaa gaggtcctga agcacgagcc gctggggatt
     5941 tccggctggc cgaatttttt gttcctcgga ggtgtggtgg cgacgatcta ttgttcgggg
     6001 caatatggct ggccttgggg cgcagccgag ctgctgatgg ccgtgctggc ggtcgctgcg
     6061 tatgtcacga cgcccgccga caaccgcaag gcgaaccagt tcaccttcgg gccgctcgtg
     6121 gaggtggcgg tgctcttcat cggcattttt gtgacgatgg cgcccgcact gcagattctc
     6181 aatgcctggg gcaagggcga gcgcgacata ttggggctgg cattcgggct gagcgaatcg
     6241 tggcattttt tctgggcgac gggcgttctt tccagttttc tggacaacgc gccgacttat
     6301 ctgacgctgg ccgccaccgc cgccggtaag agcggcgtcg cggtcgaggg aagcgcgtat
     6361 ctgaggacct ttcttgatca gggcggcgcc gactcggcgg gccttttggc cgcgatctcg
     6421 tgcggcgcgg tcttcatggg ggccaacacc tacatcggca acgggccgaa cttcatggtc
     6481 aaggcgatcg ccgaagagaa tggcgtgaaa atgcccgggt tctttggata tatggcgtac
     6541 agtggcgcga tcttgatccc gatttttatt gccgtgacgc tcatcttctt tagaagctga
     6601 tcccgcgaac tggggcttcg cagagggagt cgctcccatg accatgctcg aaaagctcct
     6661 gctgccggaa atacgggagc tgattcacga taaggacctg cccacgctgc gggaggttct
     6721 caatcgctgg ctgccggcag atattgctga cttgcttggc gacctgggtc cctacgagga
     6781 catcgttgcc tttcagtgcc tggaggtgga actggccgcc aagacgtttg cccatctcca
     6841 gcgaacgacc caggaagcac tggtcgaaac cctgcctgaa ccggagctgg cgcggattct
     6901 caaccagctc gcgcccgacg atcgcaccgc actgctcgaa ccgctgccgc atcaggaggt
     6961 cgagcggctg ctgacgctgc tgacgccgga gaatcagcgc gtggcgcgct cgctgctttc
     7021 atacggccgg gggacggtgg gacgtctgat gacgcccgat ttcatcggtg tcagggaagc
     7081 ctggaatgtg agcctggtgc tggaccacgt gcgccgcgtg ggcaaggaca gcgaaacgct
     7141 gaacgcggtc tatgtctatg acgacgagca tcatctggtg gatgatattc ggatgcggga
     7201 gatattgctg gttgcgcctg agaccacggt gcggagttta atggaccggc gctatgtcgc
     7261 gctcaacgtc accgacgccg aagcaaccgc cgtcgagatg tttcgcaagt acgaccgcac
     7321 ggtgctgccc gtcatcgacc gccgcggcgt cctgctgggc gtggtaacga ttgacgacgt
     7381 gctggacgtc gccgaggaat cggcgacgcg cgagatccag aaattcggcg gtttggaagc
     7441 gctggaagag ccctac
//
LOCUS       DASUGB010000632         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1183012, whole genome shotgun sequence.
ACCESSION   DASUGB010000632 DASUGB010000000
VERSION     DASUGB010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183012"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFV87_12100"
     CDS             <1..246
                     /locus_tag="VFV87_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX5104549.1"
                     /translation="FGTLDELFDALTLRQVNRMQPIPIILYGKEYWDRVIDFQFLADE
                     GTIRDEHLSLMTYADDPHEAWEIIRKFYRMPEHMARD"
     gene            complement(300..1010)
                     /locus_tag="VFV87_12105"
     CDS             complement(300..1010)
                     /locus_tag="VFV87_12105"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5104550.1"
                     /translation="MARFLVVGCLLTSAVAVAEDPKLSPRHVSVTGTAIARVQPDTVV
                     WNVTIRRTDQDLATAQKACDEGVRKVLALRNELKLKSEDVQTGYLSVEKVYDRDRAGN
                     ITSFRHFEVVRTVTLRQHDTTQFDDVLARLVGAADIEVSHHLESSEYHKLRAQTRLEA
                     VKAAREKAAAMTELLGAKLGRVLRIAEPRENWSSPGFASNMAFSAPRQAEPDEAPGTF
                     APGAIEVRVSIETDFEVE"
     gene            1126..2115
                     /locus_tag="VFV87_12110"
     CDS             1126..2115
                     /locus_tag="VFV87_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX5104551.1"
                     /translation="MSPKEPARSTRRHFLHAAAAASAGMLLAGCSNSGGPQAGRLEAV
                     WGRRGTSPGMLNKPRAIAIDKDDLLYIVDFTPHIHVFTGDGGYVRGWQPPVFTNGRPS
                     GLAFNNAGNLLVADTHNYRMLVYTPQGKLLDEQTIGGTCGFGDGEFQFVTDAAQDSQG
                     NYYITQYGEFDRIQKFSPEGQFLLAWGEHGSELGQFMRPQKIVIDQADLVWVTDACNH
                     RVQVFDARGSEAKLIQHWGTVGREPGQLRYPYDILLDNAALAGQPDGRVYLCEFGNHR
                     VQKFTTDGQFVGLFGENGHREGQLDQPWGIARDSQGRMFVLDSYNHRVQRFRL"
     gene            2335..>4269
                     /locus_tag="VFV87_12115"
     CDS             2335..>4269
                     /locus_tag="VFV87_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5104552.1"
                     /translation="MFGLRIAFDQPLWLLLLILVPILWIFSYRSLSGLGPYRRVFALL
                     FRTVVFTLLVLALAGVQLQKISDRVTVIYLLDQSQSIPKRTREAMLQYVVKDVATHRR
                     AGDLQSASEDKAGVIIFGREATIEHPPFADEIRSVGMLESLFELRTDATNIASALKLA
                     QASFPEDTAKRIVIVTDGNENLGDARTIARTLTDNGIGIDVVPIKLNALAEIAVEKVA
                     LPSDIRRGQPLDARVVIENYTRPTKENPTGEVKGRLRLIRRYGNQDELLGDGEVPVVL
                     KPGKNVFTFTHTIDIPAGYTYRADFIPDDADSDVQAQNNSATAFTHVRGKGRVLLIED
                     WENPREFEFLVQRLGEMNIEVEVMSSDNLFTSLAELQGFDCVVMGNVPRASGGGEATT
                     REATNFTDDQIAMLVRNTEQFGCGLIMLGGPTSFGAGGWTNTELEKAMPVDFQIKNAK
                     VKAVGALVMMMHASEMAQGNYWQKIIGQEALKVLGPSDYCGVVHWDNFTGNDNWLWRV
                     SGKGLARVEDRQKIWLAKFDRMQPGDMPAFEPAMNLALKEFIPNPASVKHMIIISDGD
                     PSPPSGTILSQYKRNSIRISTCAVGSHGPATSKVLQDIATASGGKYYAVTNPKALPRI
                     FQIEARRVARPLVKEKAAGMP"
BASE COUNT          826 a         1426 c         1277 g          740 t
ORIGIN      
        1 ttcggcacgc tcgacgagct gttcgacgcg ctgacgctcc gccaggtcaa ccgcatgcag
       61 ccgatcccga tcattcttta cggcaaagaa tactgggacc gcgtgatcga tttccagttc
      121 ctggccgatg aagggacgat ccgcgacgag cacctctcgc tgatgaccta cgccgacgac
      181 ccccacgagg cctgggaaat catccgcaag ttttaccgca tgccggagca catggcccgg
      241 gactgacacc aagcgcgacg cgcaagcgag ggagtctctt gcttacgcgt cgcgcttggc
      301 tactcaactt cgaaatccgt ctcaatgctc acgcggacct cgatcgcgcc gggcgcgaac
      361 gtgccggggg cttcgtcggg ttccgcttgc cgcggggcgg agaaggccat attgctcgcg
      421 aagccgggcg acgaccagtt ttcgcgcggc tcggcaattc ggagcacgcg ccccagtttc
      481 gcgccgagca gttcggtcat cgcggcggct ttctcgcggg ccgccttgac ggcttccagg
      541 cgagtctggg cccgcagttt gtgatattcc gacgattcca ggtggtgcga gacctcgatg
      601 tccgccgccc ccaccagccg ggccaatacg tcgtcgaatt gcgtggtgtc atgctggcgg
      661 agcgtcaccg tgcggaccac ttcgaaatgg cggaacgacg tgatgttccc cgcccggtcg
      721 cggtcataca ctttctcgac cgacaggtag ccggtctgca cgtcttccga cttgagcttc
      781 aactcattcc gtagcgcgag cactttgcgg accccttcgt cgcaggcctt ctgggccgtc
      841 gccaggtcct gatctgtccg ccggatcgtg acgttccaaa cgaccgtatc cggctggacc
      901 cgggcgattg cggtccccgt caccgaaaca tgccgcggcg aaagctttgg gtcctcggca
      961 actgcaacgg ccgaagtgag caagcaacca acgacgagaa atcgcgccat gcgagcacct
     1021 cagatcagag tgagagggag gattggcagc ctggagtgta gcctgcgtcg gcgggtcgct
     1081 gcgtaaagac ttcgtaacag ccctccgctc gcctagaatg agagcatgtc accgaaggaa
     1141 ccggcgcgat cgacccgtcg tcattttctg catgctgcgg ccgcggcgag tgccgggatg
     1201 ctgctcgccg gctgctcgaa ttccggcggc ccgcaagccg gacggctgga ggcggtgtgg
     1261 ggacggcggg gaacgtcgcc cggcatgctc aacaagcccc gggcgatcgc catcgacaag
     1321 gacgacctgc tctacattgt cgatttcacg ccgcacatcc acgtcttcac gggagacggc
     1381 ggctatgtcc gcggctggca gccgcccgtt ttcaccaacg gccgcccgtc gggcctggcc
     1441 ttcaacaacg ccggcaatct gctggtcgcc gatacgcaca attaccgcat gctcgtctat
     1501 acgccccagg gcaagctgct ggatgagcag acgatcggcg gcacgtgcgg ctttggcgat
     1561 ggcgaattcc agtttgtgac cgatgccgcc caggattcgc agggaaatta ctacatcacc
     1621 cagtacggtg agttcgaccg cattcagaag ttctcgccgg agggccagtt cctgcttgcc
     1681 tggggcgagc acggcagcga gctcggccag ttcatgcggc cgcagaaaat cgtgatcgac
     1741 caggccgacc tcgtctgggt caccgacgcc tgcaaccacc gcgtgcaggt cttcgacgcc
     1801 aggggcagcg aagcgaagct catccaacac tggggcactg tagggagaga acccggccag
     1861 cttcgctatc cttacgacat cctgctcgac aacgccgcgc tcgctggaca gcccgatggc
     1921 cgcgtgtacc tctgcgagtt cggcaatcac cgcgtccaga agttcacgac cgatggccag
     1981 ttcgtgggcc tgttcggcga aaacggccac cgcgaggggc agctcgacca gccctggggc
     2041 atcgcccgcg attcccaagg ccggatgttc gtcctcgatt cctacaacca ccgcgtgcag
     2101 cgcttccgcc tgtagcctgt agggtgggct gtgcccacca attccaaaag gcattgtcgc
     2161 agtcctggtg ggcagagccc accctacact gctcgtttaa cgcgaagccg aaggcgccgc
     2221 ggaaatcttg tcggcatcgc ctgcggctcg ccgttaaacg aatactagga aacgtggcgg
     2281 caacccccta gaatgacaat ctgtctgaaa ttcgcctaag ctgaagagat cgccatgttc
     2341 ggcctccgaa tcgccttcga ccagccgctg tggctgttgc tgctcatcct ggtgccgatc
     2401 ctgtggatct tcagctaccg gagcctttca ggactcggtc cctaccggcg cgtcttcgcc
     2461 ctgctcttcc gcacggtcgt cttcacgctg ctcgtgctgg cgctggccgg cgtgcagctt
     2521 cagaaaataa gcgaccgggt cacggtcatt tacctgctcg accagtcgca gagcattccc
     2581 aagcgcaccc gcgaggcaat gctgcagtac gtggtcaagg atgtcgccac tcaccgccgc
     2641 gccggcgacc tgcagagcgc gtcggaggac aaagccggcg tgatcatctt cggccgcgaa
     2701 gccaccatcg agcatccgcc gttcgccgac gaaatccgct cggtcggcat gctcgaaagc
     2761 ctgttcgagc tgcggaccga tgctacgaac atcgcctcgg ccctcaagct tgcacaagcc
     2821 tcattccctg aagacaccgc taagcgaatc gtcatcgtca ccgacggcaa cgagaacctg
     2881 ggcgacgccc gcacgatcgc ccgcacgctc accgacaacg gcatcggcat cgacgtggtg
     2941 ccgatcaagc tgaacgccct ggccgagatc gctgtcgaaa aggtcgcctt gcccagcgat
     3001 atccgccgcg gccagccgct cgatgcccgc gtcgtgatcg agaattacac ccggcccacg
     3061 aaggagaacc ccaccggcga ggtcaaaggc cgcctgcgct tgattcgccg ctacggcaat
     3121 caagatgaat tgctcggcga cggcgaagtc cccgtcgtcc tcaagccggg caagaacgtc
     3181 ttcacgttca cccacacgat cgatatcccc gccggctaca cctaccgggc cgatttcatt
     3241 ccggacgacg cggattcgga cgtgcaggcc cagaacaaca gcgccaccgc cttcacacac
     3301 gtccgcggca aggggcgcgt gctgctcatc gaagattggg agaatccccg cgaattcgag
     3361 ttcctcgtcc agcggctggg cgagatgaac atcgaggtcg aggtgatgtc cagcgacaac
     3421 ctcttcacca gcctggccga gctgcagggc ttcgattgtg tcgtcatggg caacgtgccg
     3481 cgggccagcg gcggcggcga agcgactacc cgcgaggcca ccaacttcac cgacgaccag
     3541 atcgccatgc tcgtccgcaa taccgagcag ttcggctgcg gcctgatcat gctcggcggc
     3601 ccgaccagct tcggcgccgg cggctggacc aataccgagc tggaaaaagc gatgccggtc
     3661 gatttccaga tcaaaaacgc caaggtcaag gcagttgggg ccttggtgat gatgatgcac
     3721 gcctcggaaa tggcccaagg caattactgg cagaagatta tcggccaaga ggcgctgaag
     3781 gtgttggggc ccagcgacta ttgcggcgtc gtccactggg acaacttcac gggcaatgac
     3841 aactggctgt ggcgcgtcag cggcaaaggg ctggcccgcg tcgaggatcg gcaaaaaatc
     3901 tggctcgcca agttcgaccg catgcagccg ggcgacatgc ccgcctttga gccggcgatg
     3961 aacttggccc tgaaggagtt catccccaat ccggcctccg tcaagcacat gatcatcatc
     4021 agcgacggcg acccctcgcc ccccagcggt acgatcctgt cgcagtacaa acgcaacagc
     4081 atccgcatct cgacctgtgc cgtcggctcg cacggcccgg caacgagcaa ggtgctgcaa
     4141 gacatcgcga ccgcctccgg cggcaagtac tacgccgtca ccaatcccaa ggccctgccg
     4201 cgaatcttcc agatcgaagc ccgccgcgtc gcccgtccgc tggtcaagga aaaggcggcc
     4261 ggcatgccg
//
LOCUS       DASUGB010000633         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_626557, whole genome shotgun sequence.
ACCESSION   DASUGB010000633 DASUGB010000000
VERSION     DASUGB010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626557"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFV87_12120"
     CDS             complement(<1..405)
                     /locus_tag="VFV87_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5104553.1"
                     /translation="MFPLPQLPPRNPDSHKGDFGRALLIGGSRGMAGAIALAGIACLR
                     SGAGLVRVAAPEDAADTVARFETSYMVTPLAQQQGGELDLSAAQREIEKLLEWATCIA
                     IGPGLGQSQPRGALVRWLYKTAKQPVVVDADGL"
     gene            complement(588..1505)
                     /locus_tag="VFV87_12125"
     CDS             complement(588..1505)
                     /locus_tag="VFV87_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5104554.1"
                     /translation="MRHLFLSLLLLLPLTSLADEPLAKKVLFIGLDGTRFDAIEAAKT
                     PHLHALMKDGIHSPQCLILGDRYQKNDTISGPGWSSILTGVWADKHGVHDNSFKGSHY
                     DEFPHFFARLKEVRPDVKTASYVTWEPIHKLIVSAADESKNFEQKEHTTADYDRYDHE
                     AAAATAKQLKEGNPDALFFYVGQIDVAGHTSGFHPSVPQYIAAIERGDALVGEVVAAM
                     KARPTFAKEDWLVIVTADHGGKGTGHGGGHKIPEILHSFLIVSGPSAQRGTFPEQVYL
                     PDAPVTALAHLGVEAKKEWKLDGRPVGLK"
     gene            complement(1602..1991)
                     /locus_tag="VFV87_12130"
     CDS             complement(1602..1991)
                     /locus_tag="VFV87_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104555.1"
                     /translation="MLHLFGVMTVVCLSAAAFYWLPSAGPSISFLFFVAIALFYRTRA
                     VVRTAVPSLRQRSISDLITFGIATSLVVCLAAFAPFWFTYTLADLYIRIMQPSHPELY
                     ILAACFPGILLAMLVLYRNWPQPPRSH"
     gene            complement(2082..>3100)
                     /locus_tag="VFV87_12135"
     CDS             complement(2082..>3100)
                     /locus_tag="VFV87_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5104556.1"
                     /translation="TVLGAGKSFFDFRPGLSLSHYRQDYELDTPNEKMKVVGHVEQMH
                     QSACYRGAKTFSCLTCHNPHGEPKGKDSAAHYLRVCTSCHEPAECRVSPAVRAKESPA
                     NDCIACHMPRTATDIPHLAFTHHRVGLHVVKKSEEHPATPRQFAGTLHPMFDLSHLSP
                     SEQKRSLGLAYFELLAGEAHDDVRRHYNREALQLLTQSREGRNVDGVADAMIARLRRQ
                     ADLPDVQRFVQAALGDSQLTGQDLCNALFLAAESQMQQKRFHDALATLDRMNRLRRNS
                     TQWLLKAECQQALGDERAREQALLAAVHINPRLWKTHAQLAEIYRRRGDAAQARFHEL
                     RGVP"
BASE COUNT          590 a          901 c         1037 g          572 t
ORIGIN      
        1 cagcccgtcg gcatcgacca ccaccggctg cttcgcggtc ttgtagagcc agcggacgag
       61 cgccccccgc ggctgggatt gccccagccc cggcccgatc gcgatgcatg tcgcccactc
      121 cagcagcttt tcaatctccc gctgggccgc ggacaagtcc aactcgccgc cttgttgttg
      181 ggccagcggc gtcaccatgt aggacgtctc aaaccgggcg acggtatcgg ccgcatcctc
      241 cggcgcggca acccgcacga ggcccgctcc gctccgcagg caggcgattc ccgccagagc
      301 aatcgctccc gccattcccc gactgccgcc aatcaggagg gcgcggccga agtccccctt
      361 gtggctgtcg ggattcctcg gcggaagctg gggcagcggg aacatgaaat cagtggatcg
      421 ggaggatcga accggaaact tgcagtcaaa cgtcgctgtt gcgcattctt cagtttagtg
      481 taagacttga ttgcaggaga tatgcgtgcc cgccttgccc catccccact gttccgccga
      541 catgcaatcc agccgcgatg ccgcggagga gcgcgggaga ctcaaaccta tttgagtcca
      601 accggccggc cgtcgagctt ccattccttt ttcgcttcga cgccgagatg ggccagcgcg
      661 gtgacggggg cgtcgggaag atagacttgc tccggaaagg ttcctcgctg ggcgcttggg
      721 ccgcttacaa tcaggaagct gtgcaggatc tctgggattt tgtggccgcc gccgtggccg
      781 gtccctttcc cgccgtggtc cgcggtgacg atcacgagcc agtcttcctt ggcgaaggtt
      841 ggacgggcct tcatcgccgc gacgacctcg ccgacgagag cgtccccccg ctcaatcgcc
      901 gcgatgtatt gcggcacgct cggatgaaag ccgctggtat ggccagcgac gtcgatctgg
      961 ccgacgtaga agaacagggc gtcggggttt ccttccttga gctgcttcgc tgtcgcggca
     1021 gccgcttcat gatcgtagcg gtcgtaatcg gcggtagtgt gctccttctg ctcgaagttt
     1081 ttggactcgt cggcggcgga gacaatgagc ttgtggattg gctcccaagt gacatagctg
     1141 gcggtcttga catcgggcct gacttccttg agccgggcga agaaatgggg gaactcgtcg
     1201 tagtgcgagc ctttgaagct gttgtcatgg acgccatgtt tgtcggccca gacgccggtg
     1261 agtatcgagc tccagccggg gccgctaatg gtgtcgttct tctggtagcg gtcgccgaga
     1321 atcagacact gtggcgagtg gatgccgtct ttcatcaggg cgtgcaggtg gggcgttttc
     1381 gccgcttcga tggcgtcaaa gcgcgtgcca tcgaggccga tgaagaggac tttcttcgcc
     1441 aggggctcgt cggccaagga ggtcagcgga aggaggagca gcagggaaag gaagaggtgt
     1501 cgcatggcgt gcgaagatgg gaaagaggct ggcggagttg tggatggcag aatcataaga
     1561 ggccgcgaca ggagatggaa tcaggcgggc gcggcgaaac actaatgcga ccggggcggc
     1621 tgcggccaat tgcggtaaag aacgagcatc gcaagtagga ttccggggaa gcaggcggca
     1681 aggatataca actcgggatg ggacggctgc atgatgcgga tgtagaggtc ggccaaggta
     1741 tacgtgaacc agaacggagc gaaggccgcc aggcagacga ccagggacgt ggcgatgcca
     1801 aacgtgatca aatcggaaat cgaacgctgc cgaagcgaag gaactgccgt gcgaacgacg
     1861 gcccgcgtcc ggtagaacaa ggcgatggcc acaaaaaaga ggaaggagat cgacggcccg
     1921 gctgagggca gccagtagaa ggcggccgcg gaaaggcaca cgaccgtcat cactccaaac
     1981 aagtgcagca acgaaaactg ccaggggcga ctttcgtcgg actggtttgt cgtttcgggt
     2041 tgtgacgctg gggccatcgt cgaacaaggg ggcggaaaaa cttatggcac tccccggagc
     2101 tcgtgaaatc tggcctgcgc cgcatctcct cgccggcgat agatttccgc gagctgcgcg
     2161 tgggttttcc agagccgggg gttgatgtga acggcggcga gcaaggcttg ctcgcgggcc
     2221 cgttcatcgc cgagggcctg ctggcactct gctttgagca gccactgcgt gctgttgcgg
     2281 cgaagccggt tcatgcggtc cagcgtggca agggcgtcgt gaaaacgctt ctgctgcatc
     2341 tgggattcag cggcgagaaa cagggcgttg cacaaatcct gcccggtgag ctgggaatcg
     2401 cccagcgccg cttggacaaa tcgctgcacg tccggtaaat ccgcctgccg gcggagccgg
     2461 gcgatcatgg cgtccgccac gccatccaca ttcctcccct cgcgagactg cgtcaggagc
     2521 tgcagcgctt cacggttgta gtggcggcga acatcgtcat gggcttcgcc ggccaagagc
     2581 tcaaagtagg ccaggccgag cgagcgcttc tgctcgctcg ggctgagatg ggaaagatca
     2641 aacatcgggt gaagcgttcc cgcaaattgc cgcggagtcg ctggatgttc ttcagacttc
     2701 ttcacgacat gaagccccac ccggtggtgc gtaaacgcca gatgggggat gtccgtcgcc
     2761 gtccgcggca tatggcaggc aatgcaatcg ttagccggac tttccttcgc ccgcaccgcc
     2821 gggctcacgc ggcactcggc cggctcgtgg cagctggtgc agacccggag gtaatgggcg
     2881 gcgctgtcct tacctttcgg ctcgccgtga ggattgtggc aggtgaggca cgaaaacgtc
     2941 ttcgcgcccc ggtagcaggc actctggtgc atctgctcga cgtgtccgac gaccttcatc
     3001 ttttcgttgg gcgtatcgag ctcgtagtcc tggcggtagt gcgatagaga cagcccgggg
     3061 cgaaaatcaa aaaaggactt tcccgctccc aggacggtcg
//
LOCUS       DASUGB010000634         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1321730, whole genome shotgun sequence.
ACCESSION   DASUGB010000634 DASUGB010000000
VERSION     DASUGB010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321730"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VFV87_12140"
     CDS             complement(<1..1662)
                     /locus_tag="VFV87_12140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5104557.1"
                     /translation="MCLFATGIESPPELPERIGRYRILRLLGEGGMGSVFEAEQEQPR
                     RTVALKVIRSGVLSAGMLQRFETESQALARLHHPGIAQVYEAGAADYGWGSQPYFAME
                     FIEGVPLDRYAGLHQLDTRQRLALMIQICEAVEHAHRRGVIHRDLKPGNILVEENGQP
                     KILDFGLARVTDSDVQATRQTDVGQLLGTLAYMSPEQVLADPSAVDTRSDVYALGVIL
                     YELLAGKLPYAMSRKLHEAVLTIREQDAAPLSAVSRIYRGDVETIVAKALEKDRERRY
                     SSAAGLAADMRRYLEDQPISAKPPSTAYQLQKFARRNRALVIGVAAVFVVLIAGVLAS
                     MREAARARSAERRALAAQQAATRDRDRAVKAEFQAVQDRNAAQVSEAQARHDRSVAME
                     EKRRADGQAAIAAAVNAFLQNDLLAQASASRQSGRREPPNPDLKVRTALDRAAERITG
                     KFDNQPEVEAAIRSTMGESYTDLGVYPEARKQLERALELQRQVLGPGNPKTINTLHVL
                     GRTALYQGKHADAESLASQALEISRRSLGAEDPTTLTTMTGLANAY"
     gene            complement(1717..2382)
                     /locus_tag="VFV87_12145"
     CDS             complement(1717..2382)
                     /locus_tag="VFV87_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104558.1"
                     /translation="MERTDALERLCGGYWYPVYAFIRRRGNSPEQAQDLTQQFFLRIL
                     DGAFFERANPEKGRFRSFLLGAVQNFLSDASDRERSQRRGGGVLPLSLDFATGELNYA
                     REPQHTETPERIFQRKWARTLLDRVMGSLSAEFAADGRLELFHRIKGYLTGDGDLKYA
                     ELGAELNLTESGVKSAIRRMRQRYRDLLRAEVISTVADLSEVDDELQFLLRAISMQNL
                     EPR"
     gene            2579..2893
                     /locus_tag="VFV87_12150"
     CDS             2579..2893
                     /locus_tag="VFV87_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104559.1"
                     /translation="MTKMLGTALYTAAVAAMLGLPASAGQAPMAALQLQATARAAKTA
                     PERTVVAKQDRLEAGVAAAEAVKLDRSFGAMAYKWPAMANSRQPGNVTSAEARRSVAE
                     NR"
     gene            complement(3060..>3427)
                     /locus_tag="VFV87_12155"
     CDS             complement(3060..>3427)
                     /locus_tag="VFV87_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104560.1"
                     /translation="VAFLGAQLGPLNELSLDHGIFLGIRLTQQVPPAPAPLAVTHADG
                     SAVEPSKPAKVGEAINIALPNAKPGPARVLIDGVEAPSKPGATLTVTVPQLKRYGAVP
                     LAVSAPGLVHDITDLPVQP"
BASE COUNT          615 a         1041 c         1133 g          638 t
ORIGIN      
        1 gtatgcgttg gccagcccgg tcatcgtcgt gagcgtggtg gggtcttcgg cacccagcga
       61 gcggcggctg atttccagcg cctgactcgc cagggactcg gcgtcggcgt gcttaccctg
      121 atatagagcc gttcgtccga ggacgtgcag ggtgttgatg gtttttggat ttcccggccc
      181 caacacctgg cgctgcaact ccagagcacg ctccagttgt ttccgcgctt ccgggtagac
      241 tccaaggtcg gtgtaggact cccccatggt gctccgaatg gcagcctcga cctccggctg
      301 gttgtcaaac tttccggtga tgcgttcggc ggcgcgatcc agggcggttc gcactttcag
      361 atcgggattg ggcggttcgc ggcgtcccga ttgccggctg gcgctagcct gcgccagaag
      421 atcgttctga agaaatgcat tcacggcggc tgcgattgcc gcctggccgt cggcgcgcct
      481 cttctcctcc atcgccacgc tgcggtcgtg gcgagcctgc gcttcggaga cctgtgcggc
      541 attgcgatcc tgaacggcct ggaactcagc ctttaccgca cgatcgcgat ctcgagtggc
      601 agcctgctgg gccgccagcg cccggcgttc cgcggagcgg gcgcgcgccg cttctctcat
      661 gcttgccagg actccggcaa tcaagactac gaacactgcc gccacgccaa tgaccagcgc
      721 tctgttccgc cgagcgaact tttgcaactg gtaggccgtg ctcggcggct tggccgatat
      781 cggctggtct tccaggtacc ggcgcatatc cgccgccaga ccggcagcgg aggaatagcg
      841 ccgctctcga tccttctcca gcgccttggc gacgatggtc tcgacatcgc cgcgatagat
      901 gcggctcacg gcactcagag gcgcggcatc ctgctcacgg atcgtcagga cggcttcgtg
      961 caatttacgg ctcattgcgt acggcagttt gccggcgaga agttcgtaga ggatcacgcc
     1021 cagggcatac acatcgctgc gcgtgtccac ggccgacgga tccgcgagca cctgctccgg
     1081 actcatgtaa gccagcgtcc cgagcagttg tcccacgtcg gtttgccgcg tggcctggac
     1141 gtcgctatcg gtcacgcgcg ccaggccgaa atccaggatc ttgggctggc cgttttcctc
     1201 caccaggata ttgccgggct tcaggtcacg atggatgacg ccgcgccgat gggcgtgttc
     1261 caccgcctcg catatctgaa tcatcagcgc gagcctttgg cgggtgtcca actggtggag
     1321 gcctgcgtac cggtccagcg gcacaccctc aatgaattcc atggcgaagt agggctgcga
     1381 cccccagccg tagtcggccg cgcctgcttc atagacctgg gcaattccgg ggtggtgcag
     1441 ccgggcgagg gcctgcgact ctgtctcaaa ccgctggagc atgccggcgc tcaagacgcc
     1501 ggaccggatg actttgaggg cgacggtacg gcggggctgt tcctgttcgg cctcgaatac
     1561 cgagcccatt cctccttcgc ccagcaagcg aaggatgcga tagcgaccga tccgctcggg
     1621 caattccggc ggcgattcga ttccggtggc aaaaaggcac actccgcaca gttcgccatc
     1681 gcccaggggt tcaccgcagg tgggacatgg tttcatttat cgaggttcca gattctgcat
     1741 gctgatggca cggagcagga actgcagctc atcgtccact tcggagaggt cggcaacggt
     1801 ggagatcact tctgcacgga gcaggtcgcg atagcgttgc cgcatccggc gaattgcgct
     1861 cttgactccc gattcggtca gattcaactc ggctcccaat tcggcgtact tcagatcgcc
     1921 gtcgccggtt agatagcctt tgatgcgatg gaaaagctcc agcctgccat cggcggcgaa
     1981 ctcagccgaa aggctgccca tcacgcggtc gagaagggtt cgtgcccatt tgcgctggaa
     2041 gattcgctcg ggagtctcgg tatgctgagg ctcgcgggca taattcaatt cgccggttgc
     2101 gaagtcgagc gagagcggaa gcactcctcc gccccgcctt tgcgatcttt cgcggtcgga
     2161 tgcgtcggag aggaagtttt ggaccgcccc gagcagaaag ctgcggaaac gccccttttc
     2221 aggattcgcc cgttcgaaga aggcgccgtc cagaatacgg aggaagaatt gctgggttag
     2281 atcctgggcc tgctcgggcg agttgccgcg gcggcgaatg aaggcgtaga cggggtacca
     2341 atagccgccg cacaagcgct ccaacgcgtc ggtgcgttcc aagctggagt ggttgccccc
     2401 cgcggcgatc acaagcgtcc aatgcgtcgt tggaaatgcg ttgtgtcctg ccgcctgagg
     2461 ccccatgtga tttgagcagt atagcccaac ctatcaaaaa ttccgcctag cgcgaaactt
     2521 ttcccggcaa atacctaagt actcatcgaa agcgaggtac ttaccaatga aaatgcaaat
     2581 gacaaagatg ctcggaaccg ccctttacac ggccgcggtc gccgcaatgc tgggattgcc
     2641 cgcctccgcc ggccaggcgc ctatggctgc actgcagttg caggcaacgg cccgggccgc
     2701 gaagaccgca cccgagcgta cagtggttgc aaaacaggac cggctggaag ccggggtagc
     2761 agccgccgag gccgtaaagc tggaccgatc gttcggcgcc atggcctaca agtggccggc
     2821 aatggcaaac agccgtcagc ctgggaatgt gacaagcgcg gaagcccgcc gctcggttgc
     2881 agagaaccga tagccggcga gcagtatgcc cgcctcgccg tgcaggcgac tccgccgaat
     2941 catctctgcg actgaccaca gccaaagaac gcaccaacac ctgctcgggg cgcagaagag
     3001 tcagcggccc gggcaggcgt tccgcaggtt acacaacgaa ccagcagcgt gacgccgttc
     3061 taaggctgaa ccgggaggtc ggtgatgtcg tggacgaggc cgggggcaga gacggcgagg
     3121 gggacggcgc cgtagcgttt gagttgaggg acggtgacgg tgagggtggc gccgggtttg
     3181 gacggggctt cgacgccgtc gatgaggaca cgcgcggggc cgggcttggc gttggggaga
     3241 gcgatgttga tcgcctcgcc tactttggcg ggcttggagg gttcgacggc ggagccatcg
     3301 gcgtgtgtga cggcaagcgg cgcgggggcg gggggaacct gttgggtgag gcggatgccg
     3361 aggaagattc cgtgatcgag cgagagttcg ttgaggggcc cgagttgcgc gccgaggaag
     3421 gcgacgg
//
LOCUS       DASUGB010000635         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_626650, whole genome shotgun sequence.
ACCESSION   DASUGB010000635 DASUGB010000000
VERSION     DASUGB010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626650"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1866)
                     /locus_tag="VFV87_12160"
     CDS             complement(67..1866)
                     /locus_tag="VFV87_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5104561.1"
                     /translation="MSQTVGWDKAAEAAGPPICKLRGGPALASLAGPTLLAIFAATAG
                     AAPPKVNYLFPAGAQRGQTASVTASGEFSNWPVQVWADRPGLTATCEKDKGKLKVEVA
                     ADAVPGTYWLRLYDSEGAAVMRPFVVGTLPEVTEDETNDLPVKPQAVDARVVVNGRLA
                     KSGDLDGYRVELKQGQTLVAAVQGHSILGSPMDSVLQICELLERRSSSAVSTKVETEA
                     FVLEQNHDAIGLDPQIAFSAPRDGQYLVRLFAFPSEPDSSIRFAGGDAFIYRLTLTTG
                     GFIDHALPLAVGAETANVRLVGWNIPEALLAFAVPSVADPLTHRIFHPDLAGAVELPR
                     LEQPCILGDDAASLDKPQTVSLPVTIGWRLEKAGDADAYAFQAAKGDKVHIEVEAHAL
                     GFPTDATLAVLDDAGRALAEADDTGREQRDPQIDFTAPEEGRYRVAIRDLAGRGGPRI
                     AYRLTIEPQAPGFSLSLAADSFVLDKDKPLEIQVNVAAQNGFREEVEIQAIGLPPGVT
                     AEPIKFTPSGERGNSGGSGRRGRRGGSGDQNSGQGVKLVLKGDPALVEAGGWPIRIEG
                     RTGSEQPLVRTAEFPLNLPLAGSHHAAWLTIRK"
     gene            complement(1863..>3013)
                     /locus_tag="VFV87_12165"
     CDS             complement(1863..>3013)
                     /locus_tag="VFV87_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104562.1"
                     /translation="SHYETFDPKPEAPAEFRGQFEAIATKIPGVTFSQHMKKLAAAAG
                     KFAVIRSIRHDQGNHGAGNHYMMTGAPPRIPVGCGAFVSFHPAMGAAAAKECPTPPGL
                     PGYFSLGQMSRSGGPNFLGARYAPFVVEENPDSSNFRVRDVAIPRELTEGRFLGRMDL
                     RQLVDRMQRINEDSAADPVRDVDQFYEQGYSLVASAEAQAAFDISREDAKLRERYGRN
                     AFGQRCLLARRLVEAGVPFITVYDGGWDHHTDLFNAFTKRMPGWDASVAALIEDLHER
                     GMLESTMVIALGEFGRTPQINKDAGRDHWSNAMSVLFAGGGTPGGQAIGATDNKGFAA
                     IERVLSPENFVSTVYTKLGINPDKMYYTHEGRPVHLVSGPEPIRELMG"
BASE COUNT          487 a         1025 c          975 g          526 t
ORIGIN      
        1 tcaattgcgg caacgactaa ccatgtggaa ggtcccgacc gcaattgact cccgaccccc
       61 tggcgttcac ttccggatcg tcagccatgc cgcgtgatgc gagccggcaa gcggcaggtt
      121 gagcgggaat tcggcggtgc gcaccagcgg ctgttcgctg cccgtgcggc cttcaatgcg
      181 gatcggccag ccgccggctt cgaccagtgc tggatcgcct ttgagtacga gtttcacccc
      241 ttggccgctg ttctggtcgc cgctgccgcc gcgtcggccg cgtctgccgc tgccgcctga
      301 atttcctcgc tcgccgctcg gcgtgaattt gatcggttcc gcggtgacgc ccggcggcag
      361 accgatcgcc tgaatctcga cttcctcacg aaatccgttc tgggccgcca cattcacctg
      421 aatctccagc ggcttgtcct tgtccaggac gaacgaatcg gcggccagcg agagcgaaaa
      481 accgggcgct tgcggctcga tcgtcagccg gtaggcgatg cgcggcccgc cgcggccggc
      541 caaatcgcga atggcgacac ggtagcggcc ttcctccggc gcggtgaagt cgatctgcgg
      601 atcgcgctgc tcccggccgg tgtcgtccgc ttcggccagc gcccggccgg catcgtccag
      661 caccgccagc gtggcatcgg tcggaaagcc gagcgcatgg gcctcgactt cgatatgcac
      721 tttgtcgccc ttcgcggctt gaaacgcata cgcatccgca tcgcccgcct tctccagccg
      781 ccagccgatc gtgacgggca acgaaaccgt ctgcggcttg tcgagcgacg ccgcgtcgtc
      841 gcccaggatg cacggctgct ccaatcgcgg cagctcaaca gcaccggcga ggtccggatg
      901 aaaaatgcga tgggtgagcg ggtccgccac gctgggcacg gcgaacgcca gcagcgcctc
      961 gggaatgttc cagccgacca gacgcacatt tgctgtctca gccccaaccg ccagcggtag
     1021 cgcgtgatcg atgaagccgc cggtcgtgag cgtcaggcga tagatgaatg catcgccgcc
     1081 ggcaaagcgg atgctgctgt cgggctcgga ggggaacgcg aagaggcgga cgagatactg
     1141 cccgtcgcgc ggggcggaga atgcaatctg cggatcgagg ccgatcgcgt cgtgattctg
     1201 ttccaataca aacgcttcgg tttcgacctt ggtactgacg gccgaggacg atcgcctctc
     1261 gagcaattcg cagatttgca gcacgctatc catcggcgag ccgaggatgc tgtgcccctg
     1321 cacagccgcc accagcgttt gcccctgctt cagctccacg cgatagccat cgaggtcgcc
     1381 cgacttcgcc agccggccgt tcaccaccac ccgcgcgtca accgcctgcg gtttgaccgg
     1441 caaatcattg gtctcgtcct ccgttacctc cggcagcgtg cccacaacga acggtcgcat
     1501 gacggccgcg ccttcgctgt cgtacagccg cagccagtac gttccgggca ccgcgtccgc
     1561 cgcgacttcc accttcagct tgcccttgtc cttctcgcaa gtcgccgtca gtcccggccg
     1621 atccgcccag acctgcaccg gccagttcga gaactcgccg ctggcggtga cgcttgccgt
     1681 ttgcccgcgc tgagcaccgg cggggaagag gtagttgacc ttcggcggcg cggcgcccgc
     1741 ggtcgcggca aagatcgcga gtagggtggg accagcgagc gaagcgagcg caggcccacc
     1801 gcgaagcttg cagattggtg ggcctgcggc ctcggcggcc ttgtcccacc ctacggtttg
     1861 actcatccca tcagctcccg aatcggctcg ggaccgctca ccaggtgcac gggccggcct
     1921 tcgtgcgtgt agtacatctt gtcggggttg atgccgagct tcgtatacac ggtcgagaca
     1981 aagttctcag gcgagagcac ccgctcgatg gcggcgaagc ctttgttgtc ggtcgcgccg
     2041 atcgcctggc cgcccggcgt gccgccgccg gcaaagagga cgctcatcgc gttcgaccag
     2101 tggtcgcggc cggcgtcctt gttgatctgc ggcgtgcgcc cgaattcgcc cagggcaatt
     2161 accatcgtcg attcgagcat gccgcgctcg tgcagatctt cgatcagcgc ggcgaccgag
     2221 gcgtcccagc cgggcatccg cttggtaaac gcattgaaca ggtcggtgtg atggtcccag
     2281 ccaccgtcgt agaccgtgat gaacggcacg cccgcctcaa ccagccggcg agccagcagg
     2341 cagcgctgtc cgaaggcatt gcggccgtag cgctcgcgaa gcttcgcatc ctcgcggctg
     2401 atgtcgaacg cggcctgggc ctcggcggag gcgacgaggc tgtagccctg ttcatagaac
     2461 tgatcgacgt cgcggaccgg gtccgccgcg gagtcctcgt tgatccgctg catccgatcg
     2521 acgagctgcc gcaggtccat gcggccgagg aagcggcctt cggtcaattc gcgggggatc
     2581 gccacgtcgc ggacgcggaa attcgagctg tctgggttct cttcgacaac gaacggagcg
     2641 tagcgggcgc cgaggaaatt cggcccaccc gagcgcgaca tctggcccag cgaaaaatag
     2701 cccggcaggc ccggcggcgt ggggcattcc ttcgccgccg cggcccccat ggccggatgg
     2761 aagctgacga acgctccgca gccgacggga attcgcggcg gcgcaccggt catcatgtaa
     2821 tggttgccgg cgccgtggtt cccttggtcg tggcggatcg agcggatcac cgcgaatttg
     2881 cccgccgccg ccgccagctt cttcatgtgc tgcgagaatg tcacgccggg gatcttcgtg
     2941 gcgatcgcct cgaactggcc gcggaactcc gccggggcct ccggcttggg atcgaaggtc
     3001 tcgtagtgcg aag
//
LOCUS       DASUGB010000636         6689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1044597, whole genome shotgun sequence.
ACCESSION   DASUGB010000636 DASUGB010000000
VERSION     DASUGB010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044597"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFV87_12170"
     CDS             complement(<1..372)
                     /locus_tag="VFV87_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104563.1"
                     /translation="MNTPVLNPQPREIQARALLVGQRIDLRALGTTQRLGGGPLAVPV
                     RGGGVAILFRYGAVVLFDVQMLEEDEFLRQLRPRVQQPATDYELEQVTVRIDPEAKEV
                     LEGAAVVLKDTSNERLEIVADV"
     gene            complement(693..3866)
                     /locus_tag="VFV87_12175"
     CDS             complement(693..3866)
                     /locus_tag="VFV87_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="organic solvent tolerance protein OstA"
                     /protein_id="HEX5104564.1"
                     /translation="MNHEFSDDGVGCKYSVLSTQYSVLIAFARCAVLRQIPLIVILGV
                     LTIGFPLVVCGEVEFPAGSKSAPVVVRADGATHWTQGEYEVWALRGHCQIEQGDMVAV
                     GDEGVLWVDRADTFSGRPSKAIAYLEGSVQVNYGRKGDPHALTGQRAQSVKDKTWIGR
                     FETTGGIELAVPVTGNDSANRPPIVQRSLAAWSPRSQPPESGDPRTPNPESPSPDARF
                     QRAQFTREEIAPPVGTPLSPLGNRVRVFPRGGGRWNLRTFVDEARNERIALINSGVQI
                     VVDGVDELGTVSVETDRIVFWLPNSNLADAALSQNLQSGEGPLEFYLEGNIVFRQGDR
                     VIYADRMYYNVRQEYGVVLNAEMLTPVPEYQGLLRLKAEVLQQVNRQRFEAYGAALTS
                     SRIGVPRYWFQTQSVAIDDLQTPLIDPFTNLPLVDPATGEPQVDHQLMATSRNNFVYL
                     GGVPVFYWPTIATDLTKPTYYFDGIKIKNDSVFGTQTLLDWDLHQLLGMENVPPGTEW
                     TLSTDFLSERGPALGTNYKYAGDTLWGVPGPYHGFIDAWGIKDDGLDNLGRGRMDIVP
                     STTLRGRVLARHQQALPNDWFLMAEAGVISDFNFLEQYYELEWDTFKDETTGVYLNRL
                     ANSHSFSFAGDVRLNDFFTQTEGGRGDWFTLGQSLLFDRLTWHQHTHAGYLHMGPANP
                     PTDPQDLAVWSPLAWEADRQGLRAATRHELDLPLQAGPTKVVPYVIGEFAYWGDDLSG
                     DAISRTYLQPGVKASLPIWSANPQVSSELLNLNGLAHKLTFEADAFYAQASQDLGNFP
                     LFDPLDDDSTEAFRRRMAVNTFGQPLGTFVPMRFDERQYALRSNLQGSVSSPVEIVDD
                     LTEIRLGLNQRWQTKRGLPGQERIIDWVVLDVSAVLFPQPDRDNFGEEIGLVDYAFQW
                     HVGDRFTLLSDGFYDIFDDGLQQTTVGGILNRPQFGSLYVGYRSTEGPFSSSLLSGSL
                     SYRMSEKWIATAGASWDLGPTGNIGQNVAVTRIGESFLLRFGVNFDVSRNNLGVNFLL
                     EPRFLSSSRLGRVGGVQIPPAGAMGLE"
     gene            4702..5058
                     /locus_tag="VFV87_12180"
     CDS             4702..5058
                     /locus_tag="VFV87_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104565.1"
                     /translation="MDADPDSADELSRSIANWGHEVRWICDSTAALRLALDHRAEVAL
                     LDVSLTEMCAYAAAHALRSEPGLRGCFLLALRWHTDRRRDPQFQPTDVDLYLAKPVDL
                     LVLETVLALEADRLSR"
     gene            5111..5473
                     /locus_tag="VFV87_12185"
     CDS             5111..5473
                     /locus_tag="VFV87_12185"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104566.1"
                     /translation="METTIYDVRNSPRIYIAADGERSVYTWDGHAVAYIVGENVFGWH
                     GKHIGWFVADILYDIKGHRIGFTATTCRADTYAEPVKYTKFTKAERFKPQVPYRRPVF
                     SQLHSNQDIDAFIRQNAP"
     gene            5497..5976
                     /locus_tag="VFV87_12190"
     CDS             5497..5976
                     /locus_tag="VFV87_12190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5104567.1"
                     /translation="MYRARIGDRVRVQCTRIKKSPPASPDTGKEFEFTVGSHAVLRGL
                     SLGVVGMAQGDHKQLRLRPKEAYGPVKENLIREVRRTRLPPQLALQVGQRLTARQRAT
                     GRRHIVRIIQVKPSSVIVDGNHPLAGKTITMEILVISVDGSSNANQSQPQFDVGGES"
     gene            6026..>6689
                     /locus_tag="VFV87_12195"
     CDS             6026..>6689
                     /locus_tag="VFV87_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5104568.1"
                     /translation="MYNPLPAAATPLNTLMAYAADLELEVDRLRRREQFLRTEAGKFL
                     LRIQHACHEHSPPPFTVNALGGISEDCRMFRGLLRDLDEPPGYHPAFDQVIDIALRPL
                     VEQVFRWQQRLHGVPGAVLRCDFQSEHLAWFPARLRHILDNLISNSLRYRDGDKGEIR
                     VGLSFQTRGSGYELQLSDNGLGMTNDRSEGALDLFFRAAPARAAGLGVGLAVVKLLVE
                     QCC"
BASE COUNT         1221 a         2140 c         2105 g         1223 t
ORIGIN      
        1 aacgtcggcc acgatctcca gccgctcgtt cgaggtgtct ttcagcacga ccgcagcgcc
       61 ttcgagcact tccttcgctt ccggatcgat gcggacggtg acttgctcca actcgtagtc
      121 ggtcgccggt tgctggaccc gcgggcggag ttggcgcagg aactcgtcct cttccagcat
      181 ttgcacgtcg aacagcacca ccgccccata gcgaaacaag atcgccacgc cgccgccgcg
      241 aaccggcacc gccagcggcc cgccgccaag ccgctgcgtc gtccccagcg cccgcaaatc
      301 gatccgctgt cccaccagca gcgcccgcgc ctgaatctct cgcggctgcg gattgaggac
      361 aggcgtgttc atcgtcggtg tagtgtggag gcggccagcg gaacaggctg gcgaggctcg
      421 cagcgaacag gctagaatca tccgctgtca gcaactgaag ctcgaatcga gagaggcagt
      481 gcgatgctgc gggcattgat catagtcctt tttgtgccgc ttgcactgcc gttggcggcc
      541 caggagcagg cggaaaagga gttccgcggg cccggctaac atcatcgcgc ggctccgcgg
      601 cgggcgggat aacgacgaat cgcactccga atgcgaccgc gacttattca tccgtcttcc
      661 ctctcgcgca gcaccgcaac ccaatcatac gcctactcca gccccatcgc cccggctggc
      721 ggaatctgca cgccgccgac gcgccccagg cggctggagg agaggaaccg cggctcgagg
      781 aggaagttga cgcccagatt gttgcggctg acgtcgaagt tgacgccgaa tcgcagcagg
      841 aacgattcgc cgatccgcgt cacggctacg ttctgcccga tgttgccggt cggccccagg
      901 tcccagctcg cgccggcggt ggcgatccat ttttcgctca tccgatagct cagcgaaccc
      961 gacaacaggc tgctgctgaa gggcccttcg gtcgagcggt agcccacgta caggctgccg
     1021 aactgcggcc tattcaaaat gccgcctaca gtcgtctgct gcagaccgtc gtcaaaaatg
     1081 tcgtaaaagc cgtcggacag gagcgtgaac cggtcaccga cgtgccactg gaacgcataa
     1141 tcgaccaggc caatttcctc gccgaagttg tctcggtccg gctgcgggaa gagcactgcg
     1201 ctgacatcca gcacgaccca gtcgatgatc cgctcctggc ccggcaggcc gcgcttcgtc
     1261 tgccaccgct ggttgagccc caggcggatt tcggtcaggt catcgacgat ctcgaccggc
     1321 gagctgaccg agccttgcag gttgctccgc agggcgtatt gccgttcgtc gaaccgcatc
     1381 ggtacgaacg tgccgagagg ttggccgaac gtgttgacgg ccatccggcg gcggaacgct
     1441 tcggtcgaat cgtcatcgag cgggtcgaag agcgggaagt tccccagatc ctggctcgcc
     1501 tgggcataga acgcgtccgc ctcgaaggtc agcttgtgcg ccaggccgtt caggttcagc
     1561 agctcgctgg acacctgcgg attggccgac cagatgggca ggctggcttt cacgccgggc
     1621 tgcagatagg tccgcgagat ggcatcgccc gagaggtcgt cgccccagta ggcgaattcg
     1681 ccgatcacgt acggcaccac cttcgtcggc cccgcttgca gcggcaggtc cagctcgtgc
     1741 cgcgtcgcgg cccgcagccc ctgccggtcg gcttcccagg ccagcgggct ccagacggcc
     1801 agatcctgcg gatcggtcgg cggattggcc ggccccatgt gcaggtagcc ggcgtgcgtg
     1861 tgctggtgcc acgtcagccg atcgaacaag agcgactgac cgagcgtgaa ccaatcgccc
     1921 cgcccaccct cggtctgcgt gaagaagtcg ttcaaacgca cgtcgcccgc gaaactgaac
     1981 gagtggctgt tggccaggcg gttcaggtac acgcctgtgg tctcgtcctt gaacgtgtcc
     2041 cactccagct cgtaatactg ctccaggaag ttgaaatcgc tgataacgcc cgcctcggcc
     2101 atcaggaacc agtcgttggg cagcgcctgc tgatggcggg cgagcacgcg gccacggagc
     2161 gtcgtcgagg gcacgatatc catgcggccg cggccgagat tatcgagccc gtcgtccttg
     2221 atcccccagg cgtcgatgaa gccatgatac gggccgggaa cgccccacag cgtatccccc
     2281 gcgtatttgt agttcgtgcc gagggccggt ccccgctcgc tgagaaaatc ggttgaaagc
     2341 gtccactccg tccccggcgg cacgttctcc atccccagga gctggtgcag gtcccagtcg
     2401 agcagcgtct gcgtgccaaa gacgctgtcg ttcttgatct tgatgccgtc gaagtagtaa
     2461 gtcggcttgg tgaggtcggt ggcgatggtc ggccagtaga agaccggcac gccgccgaga
     2521 tagacgaagt tgttccgcga ggtcgccatc agttggtgat cgacctgcgg ctccccggtg
     2581 gccggatcga ccagcggcag gttggtgaac gggtcgatca gcggcgtctg caggtcgtcg
     2641 atcgccacgc tctgcgtctg gaaccagtaa cgcggcacgc cgatgcggct gctggtgagg
     2701 gcggcgccat aggcctcgaa tcgctggcga ttcacctgct gcaagacttc cgccttcagc
     2761 cgcaacagcc cctgatactc gggcacgggc gtgagcattt cagcattgag caccacgccg
     2821 tattcctgcc gcacgttgta gtacatgcgg tcggcgtaga tcacgcggtc tccctggcgg
     2881 aagacgatat tgccctccag gtagaactcc agcggccctt cgcccgactg caggttctgc
     2941 gaaagggccg catcggccag gttcgagttc ggcagccaga agacgatgcg gtcggtctcg
     3001 accgagacgg tgcccagctc atcgacgcca tcgacgacga tctgcacgcc ggagttgatc
     3061 agggcgatcc gctcgttgcg ggcctcatcg acgaacgtcc gcaggttcca ccgcccgccg
     3121 ccgcgcggga agacgcgcac gcggttgccc agcggcgaaa gcggcgtgcc gaccggcggg
     3181 gcgatttctt cgcgcgtgaa ctgggcccgc tggaagcggg catctgggga tggggattcg
     3241 ggattcgggg ttcgggggtc gcccgattcc ggtggctgcg agcgcggcga ccaggcggcg
     3301 aggctccgct ggacgatcgg cggacggttc gcggagtcat taccggtgac gggcacggcc
     3361 agttcgatgc cgccggtcgt ttcaaagcgg ccgatccaag ttttgtcttt cacgctttgg
     3421 gcgcgctggc cggtcagggc gtgcggatcc cctttgcgcc cgtaattgac ctgcacgctc
     3481 ccttccaggt aggcgatcgc cttgctcggc cggccgctga aggtgtcggc ccgatcgacc
     3541 cacagcacgc cttcgtcgcc cacggccacc atgtcgccct gttcgatctg gcagtgcccg
     3601 cgaagtgccc agacctcgta ctcgccctgc gtccagtgcg tcgccccgtc ggcgcggacg
     3661 accaccgggg ccgacttgct gccggctggg aattcgactt cgccgcagac aaccagtggg
     3721 aagccaatcg tcaggacgcc gaggatgacg atcagcggaa tctgccgcag aacggcgcat
     3781 cgtgcgaacg caatgagtac tgagtactga gtactaagta ctgagtactt gcagccaact
     3841 ccgtcgtccg agaattcgtg attcaagttg ccgcagcctt cagaccgata ctctcgttcc
     3901 gataacggct gcccgcgcgc agcatcgaac gccgagcgca ccgcgaggcg ctcgcacgac
     3961 ggacgtcggc tgcggggaac tagcgcgggc ttcagacgag tggcttcctt gccgggaatt
     4021 catccgcccg ccggcggatg cgataagacc ttggacgaga acagcttgcg ctcgcggcgc
     4081 aggggcgcca caagcggcgg cggattatag gaacgccggg gggccgtcac aaggcaaatg
     4141 gccgcagcgc cggacgcatc gctagcaatg ctgtcgcagc gtgcaacgca gtcgggcact
     4201 acccgcccac gacgtggtgc tcctccgaga accaacgtcc tttcagggtc tgtggaggga
     4261 gaatcttctg atcggcgcac cgattgcctc gcggagtatt cagctccaaa tcgctgaatg
     4321 ggccgaattt gccattgctt tgctggttac gcggcgtagg atgctgagac aaggatcaac
     4381 ggtgccatca tcggcgctgg aaatgctctg ccaaatcgaa gcgctgcact ctgcggaagg
     4441 ctagctgcgt tgcccatagg caggctcgtt gaagcggtcg ctggcgcgga atgccccggc
     4501 cagttctctt tcttgtaggc ctttgcccgt actttctgcg ctgatgggcc aggagttcgg
     4561 gcccagggag gtcgggccat gaatctatcg cctgggagtg ctcgcaagaa cgatggtgtg
     4621 cgaatcgctc cagagcaacc agcttacgag cgcgtggata agcagtgggc tttgacaagc
     4681 cgctcgctgc gcgtactcgt cgtggacgcc gatccggact cggccgacga actgtcgcgc
     4741 tccatcgcca attggggaca tgaggtgcgc tggatttgcg acagcacggc ggccttgaga
     4801 cttgcgcttg atcatcgtgc cgaagtggcg ctgctcgacg tctcgctcac cgaaatgtgc
     4861 gcttacgccg cagcgcatgc gttgcgcagc gagccggggc ttcgcggctg ctttctgctt
     4921 gcgctccgct ggcacaccga ccgtcggaga gacccgcaat tccaacccac ggacgttgac
     4981 ttgtatctag ccaaaccggt cgatttgctc gtgctcgaaa cggtgctagc gctggaagct
     5041 gaccgcctgt cccgctgaac ctggcagcca tgccatgaat tccaacttcc tagcctgaaa
     5101 gcgaggccac atggaaacga ccatttacga cgttcgcaac agcccgcgaa tctacattgc
     5161 cgcggacggc gaacgatccg tctatacctg ggatggccac gcggtggcct acatcgtcgg
     5221 cgagaatgtc tttggctggc atggcaagca tatcggctgg ttcgtggccg acattctgta
     5281 cgacatcaag ggccatcgga tcggttttac ggccacaacg tgtcgggccg atacctatgc
     5341 cgaaccagtc aaatacacaa agttcacgaa agccgagcgg tttaagcccc aagttccgta
     5401 ccgccgcccg gtcttcagcc aattgcactc gaaccaggat atcgatgctt tcattcgaca
     5461 gaacgcgccc taagaggcgc agcgaaggga gtgcccatgt atcgcgcaag aatcggcgat
     5521 cgcgttagag tgcaatgcac tcggatcaag aagtcaccac cagcaagccc cgataccggc
     5581 aaggagttcg aattcacggt cggctcgcac gccgtcctgc ggggactgag cctgggcgtg
     5641 gtcggcatgg cgcaaggaga ccacaagcag ctcaggctcc ggccgaaaga agcctacggt
     5701 ccggtcaagg agaacctgat tcgcgaggtt cgcaggaccc ggctgccacc gcaactggcg
     5761 ttgcaagtgg gacagcggct gacggcccgc cagcgcgcca ccgggcgtcg tcacatcgtc
     5821 cgaatcattc aagtgaagcc cagctcggtc atcgtcgatg gcaaccatcc cctggctggg
     5881 aagacgatca cgatggagat ccttgtgatc tcagtcgatg gttcctccaa cgcgaaccag
     5941 tcccagccac agttcgacgt tggcggagaa agttgatcca tctccagcgt tccacacagt
     6001 cggcgaccta cttccatttc acgccatgta caacccactg cctgcagccg cgacgcccct
     6061 gaataccctg atggcctacg cggccgactt ggaactcgag gtggaccggc tgcggcgccg
     6121 cgagcagttc ctgcgaacgg aagccggcaa gttcctgctc aggattcagc acgcttgcca
     6181 tgagcattcg ccgccaccgt tcaccgtcaa tgcgctcggc ggaatctctg aagattgccg
     6241 catgtttcgc ggactgctgc gggacctgga tgaacctccg ggctatcatc cggcgttcga
     6301 ccaggtcatc gacatcgccc tgcgccctct ggtcgagcag gtatttcgct ggcagcagcg
     6361 cctgcatgga gttccaggcg ctgtgctgcg gtgcgacttc cagtcggagc acctggcctg
     6421 gtttcccgcg cggctgcgcc acattctcga caacttgatc tccaattcgc tgcgctaccg
     6481 ggacggcgat aagggtgaaa tccgggtcgg gctgtccttt cagacccgcg ggtccggata
     6541 tgagttgcaa ctctctgaca atggattggg gatgacgaat gatcgctcag agggtgcgct
     6601 ggatctcttc tttcgagctg cgcctgcccg cgccgcgggc ctgggcgtcg gactggcggt
     6661 ggtgaagtta ctggttgaac agtgctgcg
//
LOCUS       DASUGB010000637         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_626698, whole genome shotgun sequence.
ACCESSION   DASUGB010000637 DASUGB010000000
VERSION     DASUGB010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626698"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFV87_12200"
     CDS             <1..640
                     /locus_tag="VFV87_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104569.1"
                     /translation="YYPAPAATTVIPTVPAPATIRSVPLGPTVPSPPTRIPSSTTPVP
                     PAETRPSLGPPPAGSFTPSGGFGTGGSFPSGSNYPPETDPYSGPTITGPTITGSSYRA
                     GSSPSAAGGAIREPGTSGSTLAPGVRLVPDPDAQQREPAPSRAPQLLDPRDKSAASRL
                     DKWAVVPAVWPKPEPDGRLTANSDAGRYYEPVRRFVPAAAEMYDDSGWKSAR"
     gene            complement(698..1249)
                     /gene="infC"
                     /locus_tag="VFV87_12205"
     CDS             complement(698..1249)
                     /gene="infC"
                     /locus_tag="VFV87_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5104570.1"
                     /translation="MVQLAHPFLQHERGRPIAERITTRINEQIRISPVRVIGPGGEQL
                     GILPVDQALTKAREADLDLVEVAPTERPPVCRIMDFGKYKYEKKKKAHQPTHHTKIKE
                     IRLRPKTGDHDIDFKIKQAKGFLQHKDKVVVSVVFRGRELAHIDEGRKVMDRVVTELM
                     EVGKLESPPMNQGKRMICTLMPK"
     gene            complement(1376..2719)
                     /locus_tag="VFV87_12210"
     CDS             complement(1376..2719)
                     /locus_tag="VFV87_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HEX5104571.1"
                     /translation="MSRILLALAALLTLPGAIQVHAADRLRLIVETDAGGDPDDEQSL
                     VRFLLYTNEWNVEGIIANRPKAREGENKNTERTGLGIVRRQIKAYGECYGKLGQHDQR
                     FPQPEDLLQRAVAGYDDVDDGVKLVIEAVDRDDPRPVWFLNWGTDNGAAESCLKRALD
                     RVLAERGKEGYARFKRKLRLASDDQFDEHTTKLEPPFPLWVDTFRPPRDGKRWYHRFS
                     KLTREADGFDIERDVRTGHGPLGALYPSNTTHRQKEGDSPTFIYLIPTGLSDPEHPTW
                     GGWPGRYGPHDEHPGKPYYWANLEDAWQGKTNRDNTLLRWAAAIQNDFAARMDWCVRD
                     FADANHPPQPKVAGELVRSMKPGERITLDALATTDPDGDPLDFAWFFYPESTGYTGEL
                     PKIDGSDASRPSFVAPARPATAKEPAELHLILAVTDRGEPPLTRYARLMLEMNGP"
     gene            complement(2732..>3035)
                     /locus_tag="VFV87_12215"
     CDS             complement(2732..>3035)
                     /locus_tag="VFV87_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104572.1"
                     /translation="QLRQPSGQVDHRQPQLTPDPLDANPKRERGTGRAICLNAQGELI
                     LVRLSPAGLIEDSRAKIIGETWAHPAYAGNHCFARDDERIVCVRIASPGDQAEAGR"
BASE COUNT          476 a         1027 c          960 g          572 t
ORIGIN      
        1 gtactatccc gctccggccg cgacgacggt cattccgacg gttccagcgc cggcgacgat
       61 ccgatcggtg ccgctcggtc caaccgttcc ttcgccgccc acgcggattc cctcctcgac
      121 aacgcccgtg ccgcccgcag aaacgcgccc cagcctcggc ccgccaccag ccggcagctt
      181 cacgccttcc ggcggcttcg gcacgggcgg cagtttcccc tccggctcga attatccgcc
      241 ggaaacggac ccgtattccg gccccacgat caccggcccc accatcaccg gttccagcta
      301 tcgcgccggc agctccccct cggctgctgg cggggcgatt cgcgagccgg gcacaagcgg
      361 cagcaccctc gcccccggcg tgcgattggt tcccgacccg gacgcgcagc aacgtgagcc
      421 ggcccccagc cgggcgccgc aacttctgga tccgcgcgac aaatcggccg cgtcgcgact
      481 cgacaagtgg gccgtcgtgc ccgccgtgtg gccgaagcca gaaccggacg gtcgcttgac
      541 ggccaactcc gacgccggcc gctactacga gcccgtgcgg cgtttcgtcc ccgccgccgc
      601 cgagatgtac gacgacagcg gctggaaatc ggcgcggtag ggtgcccgct ggactggatc
      661 gttgctgatt caggccccga cggagtcggg ggcctaccta cttcggcatc agcgtgcaga
      721 tcatccgctt gccctggttc atcggtggcg attcgagctt gccgacttcc atcaattcgg
      781 tcacgacgcg gtccatcacc ttgcgcccct cgtcgatgtg ggccagctcg cggccgcgaa
      841 agaccaccga gaccacgacc ttgtctttgt gctgcaaaaa tcccttggcc tgcttgatct
      901 tgaagtcgat gtcgtgatcg cccgttttgg ggcgcaggcg gatttccttg atcttggtgt
      961 ggtgcgtcgg ctgatgagcc ttctttttct tctcgtattt gtacttgccg aaatccatga
     1021 tccggcagac gggcgggcgc tcggtcggcg cgacctccac cagatcgagg tccgcctcgc
     1081 gggctttcgt cagcgcctga tcgacgggca gaatccccag ttgttcacct ccaggcccaa
     1141 ttacccgcac cggagagatg cggatttgtt cgttaattcg tgtcgtaatc cgttcagcga
     1201 ttggtcttcc cctttcgtgc tgcagaaatg gatgtgccaa ttggaccact ggccgcggcg
     1261 aaacgttcgc tgcggcacgg cacaattggc gtcaatcggt ctaggaaaat atcgcctaaa
     1321 tcgggcagtt ttgcaaatgg ggaacgccca aagccgcgac cattggtcgc ggttcctacg
     1381 ggccattcat ttccagcatc agccgtgcat atcgcgtcag cggcggctcg ccccggtcgg
     1441 tgacggcgag gatcaggtgc agttcggctg gttcttttgc ggtggcgggg cgagcgggag
     1501 ccacgaacga cggccggctg gcatcgctcc cgtcgatctt cggcagctcg cccgtatagc
     1561 ccgtcgattc gggatagaaa aaccaggcaa agtcgagcgg gtccccgtcg ggatcggtcg
     1621 tggccaaggc gtcgagcgtg atgcgctccc ccggtttcat ggagcgtacg agttcgccgg
     1681 cgactttcgg ctgcggcggg tgatttgcat cggcgaaatc gcggacgcac cagtccatcc
     1741 gggccgcgaa gtcgttctgg atcgccgccg cccagcggag cagcgtgttg tcacgattcg
     1801 tcttcccctg ccaggcgtct tccagattag cccagtagta cggcttgccg ggatgctcgt
     1861 cgtgcgggcc gtatcgccct ggccagccgc cccacgtcgg atgctcggga tcggacaggc
     1921 cggtcggaat gaggtaaatg aacgtcgggc tgtccccttc cttctgccgg tgtgtcgtgt
     1981 tcgacgggta aagcgcgcca agcgggccgt ggcccgtgcg gacgtcgcgc tcgatgtcga
     2041 agccgtcggc ctcgcgcgtc agcttcgaaa agcggtgata ccaccgcttg ccatcccgcg
     2101 gcgggcggaa ggtatcgacc cacagcggaa aggggggctc aagcttcgtc gtgtgctcgt
     2161 cgaactggtc atccgaagcc agccgcagct tccgcttgaa gcgggcgtag ccttctttcc
     2221 cgcgctcagc cagtacccgg tccaaggccc gcttgaggca gctttcggcc gcgccgttgt
     2281 cggtccccca gttcaggaac cagaccggcc gcgggtcgtc gcgatccacc gcctcgatca
     2341 ccagcttcac cccgtcatcc acgtcgtcat agccggcgac ggcgcgctgg agcaagtcct
     2401 ccggctgcgg gaagcgctgg tcgtgctgcc cgagcttgcc atagcactcc ccgtacgcct
     2461 tgatctgccg gcggacgatc cccaggcccg tccgctcggt gttcttgttc tccccctcgc
     2521 gggccttcgg ccggttggcg atgatgccct cgacattcca ttcattcgta tagagcaaaa
     2581 accgcaccag cgactgctca tcatccggat cgccgccggc atcggtctcg acgatcaggc
     2641 ggaggcggtc ggcggcgtga acctgaatcg cgcctggcaa cgtgagcaaa gcagcgagcg
     2701 ccaacagaat tcgggacatg gtgcgcgctc cctaacggcc ggcttcggcc tgatcgcccg
     2761 gcgaggcaat ccgcacgcag acgatccgct cgtcgtcgcg ggcgaagcag tggttgccgg
     2821 cataggcggg gtgggcccag gtttcgccga tgatcttggc gcgggaatct tcgatcagcc
     2881 ctgccggcga gagacggacg aggatcagct cgccctgggc gttgaggcag atcgcgcggc
     2941 cagtcccgcg ctcacgcttc gggttcgcgt ccaaggggtc gggagtcaat tgcggttggc
     3001 gatgatccac ctggccagac ggttgccgca attga
//
LOCUS       DASUGB010000638         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1739909, whole genome shotgun sequence.
ACCESSION   DASUGB010000638 DASUGB010000000
VERSION     DASUGB010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739909"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..>2609
                     /locus_tag="VFV87_12220"
     CDS             262..>2609
                     /locus_tag="VFV87_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5104573.1"
                     /translation="MTLPRTFVLAGLLSLASSTGFAVAQDADKPAEASASEPQAPEAK
                     PEEKKPEEKKPEEKKPDDKPAEPVAGPLPGHSYHGEVFNEGPRQKAYLMEGMPKIDFP
                     ATTKSPEAQKFIEQGIGQIHGFWYYEAERSFRQAAMLDKDCAIAYWGMALANIDNPNR
                     GKKFMDECVKHKTGLTERETLYIDALEAWFKTDNNKRKERSEAYTRALEKLLYKYPDD
                     IEARSFLALQLWKNRDAGIPISSFLAIDAVQEQVFDKNPMHPTHHYRIHLWDLEKSDK
                     ALASAALCGQCSPGIAHMWHMPGHIYSREKRYEDACWQQEASARVDHAHMMRDRVLPD
                     QIHNFAHNNEWLIRDMMFVGRMRDAIDLAKNMISLPRHPKYNTLTKGSSNYGRMRLLE
                     ALSRFEAWDELIALAGTSYLEPTDNPDEQVKRLRALGEAHYRKGDTAGGDALLADLEQ
                     RLADQKTEQEKAGAEAETKTREEKKDDKDLEKLAEKARNDAKKRFDAKINDLQKAIDE
                     LQGHKAVLAGDFKGGLDLLRKSGGASQDYLAFIQFKSGEADKAIEAARNHVKSRKNEV
                     LPLARLVEILWLADKKDDAKQTLDQLREISNSVDPSAPFYARLAPIAADLGYGADWKV
                     AKAPLPDTGVRPPLDALGPMRWSPSPALAWTAKDCDGAGHSLADFHGQPVVLIFFLGH
                     GCLHCAEQVQSFGAMAKEYSEAGIQLIAISSDDLDGLRTSIDNYKDGPVPMPLVSDPA
                     LDIFKAYRCYDDFEQQPLHGTFFIDGSGQVRWQDISYQPFNDP"
BASE COUNT          565 a          849 c          806 g          389 t
ORIGIN      
        1 ggcccgtccg gccaacagcc ccgccatgct cccgccgatc acaatcgccc gctcgccaac
       61 tcgcctgctc atcgctgcct cccaggtcaa aggggcccgg ccgaaatgcc gatcgccccg
      121 ctctacacgt cccaagcgca aagcccggcg aggtgttaca ggccgcccag aggccatgcc
      181 gaatccgatc agtggcgatc gaaggctgtc tcacgtagaa tcggggctgt cccacctacg
      241 tttcctgcct ggaattcccc aatgactctg ccccgcacct tcgtgctggc tggactgctt
      301 tcactggcca gcagcaccgg attcgccgtc gctcaggacg cggataagcc cgccgaagcg
      361 tccgcgagcg agccacaggc gccggaggcc aagccggagg agaaaaagcc ggaagagaaa
      421 aagccggaag agaaaaagcc ggacgacaag ccggctgagc cggttgccgg gccgttgccg
      481 gggcattcct accacggcga agtcttcaac gaaggcccgc ggcagaaggc gtacctcatg
      541 gaggggatgc cgaagatcga cttccccgcc acgaccaaat cgcccgaggc ccagaagttc
      601 atcgagcaag gaatcggcca gattcacggc ttctggtact acgaggcgga gcggtcgttc
      661 cgccaggctg cgatgctcga caaggactgt gcgatcgcct attgggggat ggccctggcg
      721 aatatcgaca accccaaccg cggcaagaaa ttcatggacg agtgcgtcaa gcacaagacc
      781 ggccttaccg agcgcgagac gctgtacatt gacgctctcg aagcctggtt caagaccgac
      841 aacaacaagc gcaaggagcg gagcgaagcc tatacgcggg cgctcgaaaa gctgctctat
      901 aagtatccgg acgacatcga ggcccgctcc ttcctggccc tgcagctatg gaagaatcgc
      961 gacgccggca tcccgatcag cagtttcctg gcgatcgacg ccgtgcagga gcaggtcttc
     1021 gacaaaaacc cgatgcaccc gacgcatcac taccggattc acctctggga cctggagaag
     1081 tcggacaagg ccctcgcttc ggcggcgctc tgcgggcaat gctcgccggg gatcgctcac
     1141 atgtggcaca tgccgggtca catttactcg cgcgagaagc gctacgaaga cgcctgctgg
     1201 caacaggaag cgtcggcccg cgtcgatcac gcccacatga tgcgcgaccg cgtgctcccc
     1261 gatcaaatcc acaatttcgc gcacaacaac gagtggctga tccgcgacat gatgttcgtg
     1321 ggccggatgc gggacgcaat cgacctggcc aaaaacatga tctcgctgcc gcggcacccc
     1381 aagtacaaca cgctcacgaa ggggagctcg aattacgggc ggatgcggct gctcgaagcg
     1441 ctctcacggt tcgaggcttg ggacgagctg atcgccctgg cgggcacgtc gtacctggag
     1501 ccgaccgaca atcccgatga gcaggtcaag cggctgcggg cgctgggcga ggcccattat
     1561 cgcaagggag acacggcggg cggcgacgca ctgctcgccg atctggagca gcgcctggcc
     1621 gatcagaaga cggagcagga aaaagccggc gccgaagcag agaccaagac gcgcgaggag
     1681 aaaaaggacg acaaagacct cgaaaagctc gcggagaagg cccggaacga cgcgaaaaag
     1741 cgattcgacg ccaagatcaa cgacctgcag aaggcgatcg atgaattgca gggtcacaag
     1801 gcggtgctcg ccggcgattt caaaggcggg ctggacctgc tgcgcaagag cggcggcgcc
     1861 agccaggatt atctggcttt catccagttc aaatccggcg aagccgacaa ggcgatcgaa
     1921 gcggctcgca atcacgtcaa gtcacggaag aacgaagtcc tgccgttggc tcgcctggtg
     1981 gagatcctgt ggctcgcgga caaaaaggac gacgcgaagc agaccctcga ccaactgcgg
     2041 gagatttcga actccgtcga tccgtcggcc ccgttctatg cccgccttgc gccgatcgca
     2101 gccgacctgg gctacggggc ggattggaaa gtcgcgaagg ccccgctgcc ggataccggc
     2161 gtgcggccgc cgcttgatgc gctcgggccc atgcggtgga gcccctcccc cgcgctggct
     2221 tggaccgcga aggactgcga cggcgccggc cattcgctgg ccgatttcca cggccagccc
     2281 gtcgtgttga tcttcttcct cggccacggc tgcctgcact gtgccgagca ggtgcagtcg
     2341 ttcggcgcca tggccaagga atatagcgaa gccggcatcc agctcatcgc catcagcagc
     2401 gacgatttgg acggcctgcg gacgtcgatc gacaactaca aagacggccc cgtcccgatg
     2461 ccgctggtct ccgatccggc gctggacatc ttcaaggcct accgctgcta cgacgacttc
     2521 gagcagcagc cgctccacgg cacgttcttc atcgacggca gcgggcaggt tcgctggcag
     2581 gacatcagct accagccgtt caacgatcc
//
LOCUS       DASUGB010000639         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1183475, whole genome shotgun sequence.
ACCESSION   DASUGB010000639 DASUGB010000000
VERSION     DASUGB010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183475"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..348
                     /locus_tag="VFV87_12225"
     CDS             64..348
                     /locus_tag="VFV87_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104574.1"
                     /translation="MLRYHDFVPKQTKEWGFLSPAEYESFDAALAAANKWIKENEIKV
                     LNIETVALPNIHGRFEEGSADPALPTPHGSSQTWHQFIRVWFEVGALEDS"
     gene            complement(360..2192)
                     /locus_tag="VFV87_12230"
     CDS             complement(360..2192)
                     /locus_tag="VFV87_12230"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5104575.1"
                     /translation="MSLPADHRPEQARRFRRGVGLWPATVHCTIALIAASAFADEQPS
                     KSLTPLKDRPLLRRPIAAAWIESGKLLAVANQRSGTISIVDIENRTVLDEIAVGERLA
                     DITAGPRCSRLFAVDDKRHELIVLDGDGNSLRVVNRVAVSPFPVSVAVSPDGSRITVA
                     SLWSRKLTVFEPNVDLPAEAVQLRSLAEISLPFAPRSQCFLHGGSQLVVADSFGGKLA
                     CIDIRSLRIDGIREIDGHNIRGLAATGDEQSLMVAHSLLDQNIAITRDNSVAEELMSP
                     VLTQISLAKDGPNALTGEATTRLLEDRWSIMGGSDPQGLVRTDTGKLAFVLGGSHQIA
                     FLNADGRGEGAHSVRLNPVAVTCSDANAIVVCRLSDSLVVVPTHRKEVDEIKLGTDPE
                     FSPADRGERKFYSGNISAGRWMSCHSCHTDGHTCGLLADTLGDATYGTPKRIPTLLGT
                     RLSDPWAWNGGVRELHEQVQKSLENTMHAPKFTPQDVLDITAFLHTLPPPPPLEPATK
                     SPKDIEQFARGEKLFTGLGCGKCHVPPLTYTSAEAYDVGLEDEKGLKKFNPPSLRGVS
                     QGYSFFHDGRAKSLEAVFTEHGHQLPRPLEKAELADLLRFLRSL"
BASE COUNT          455 a          737 c          736 g          459 t
ORIGIN      
        1 ggtgggtggt aggatgaatt gacgactcac ccactcaccc actccaccac tcaccggccc
       61 cccatgctcc gctaccacga cttcgtcccc aagcagacca aggaatgggg ctttctttcc
      121 cccgcggagt acgagtcgtt cgacgccgcg ctggctgccg ccaacaagtg gatcaaggag
      181 aacgaaatca aggtcctgaa catcgagacc gtcgccctcc ccaacattca cggccgcttc
      241 gaagaaggct ccgccgatcc cgccctcccc acgccccacg gcagctcgca aacctggcac
      301 cagttcatcc gggtatggtt tgaggtgggc gccctggaag attcgtagcg acttcacggc
      361 tacaaacttc gcaggaatcg caatagatcg gccagctccg ctttttccag cgggcgtggg
      421 agctgatggc cgtgctcggt gaagacggct tcgagcgatt tcgcgcggcc gtcgtgaaag
      481 aagctgtaac cctggctgac gccgcggagc gagggcgggt tgaacttctt cagccccttc
      541 tcgtcttcca ggcccacgtc gtacgcctcg gccgacgtgt acgtcagcgg cggcacatgg
      601 catttgccgc agccgaggcc ggtgaagagt ttttcgccgc gggcgaactg ttcgatatcc
      661 ttcggcgact tcgttgcggg ctcgagcggc ggtggcggcg gcagcgtgtg gaggaaggcg
      721 gtgatatcga gcacatcctg cggcgtgaac ttcggcgcgt gcatcgtgtt ttccagcgac
      781 ttttgtacct gctcgtgcaa ctcccgcacg ccgccgttcc acgcccaagg atcggacagc
      841 cgcgtcccca agagcgtcgg aatccgcttc ggcgtgccat acgtcgcatc gcccagcgta
      901 tcggccaaga ggccgcaggt atggccgtcc gtgtggcagc tatggcagct catccagcgg
      961 ccggccgaga tattgccgct gtaaaacttg cgttcgccgc gatcggccgg cgaaaactca
     1021 gggtcagtcc ccagctttat ctcatcgact tccttacgat gagtgggcac gacgaccaac
     1081 gaatcgctaa ggcggcacac aacaatggcg ttggcatcag aacaagtgac ggcgactggg
     1141 ttgagacgca ccgaatgtgc gccttcgcct cgaccatccg cgttgagaaa cgcaatttga
     1201 tgactgcctc caagcacgaa agccagtttg ccggtatcgg tccgaaccaa gccctggggg
     1261 tcgctgccac ccatgatcga ccagcggtcc tcaagcagcc gagtcgtcgc ttctcccgtc
     1321 agtgcgttgg gcccatcttt ggctagtgaa atctgtgtca ggacgggcga catcaactct
     1381 tcggcaactg agttgtcgcg cgtgatcgcg atgttctgat cgagcaacga atgcgccacc
     1441 atcagcgatt gttcgtcgcc tgtagcggct aacccccgga tgttgtggcc gtcaatctcc
     1501 cgaatgccgt cgatgcgcag cgaccggata tcaatgcacg ccagttttcc gccaaacgaa
     1561 tcggcaacga caagctgact gccaccgtgg aggaagcatt gtgaccgtgg tgcgaatgga
     1621 agggaaatct ccgcgagcga gcgaagttgc accgcttcgg ccggcaggtc cacattcggc
     1681 tcgaaaaccg tcagtttccg cgaccaaagc gaagcgacgg tgatccggct gccgtcgggc
     1741 gataccgcca cggagactgg aaatggactg acggcgacgc gattgacgac acgcagcgag
     1801 ttgccgtcgc cgtcgaggac gatcagctcg tgacgcttat catcgacggc gaagaggcgg
     1861 ctgcagcggg gaccggctgt aatgtcggcc aagcgctcgc cgacggcaat ttcgtcgagg
     1921 accgttcgat tttcgatatc gacaatcgat atcgttccgc tgcgttggtt ggcgaccgcg
     1981 aggagcttgc cgctttcgat ccacgcggcg gcgatggggc ggcggaggag cgggcggtct
     2041 ttaaggggcg taagggactt gctcggctgc tcgtcggcga aggctgatgc ggcgataagt
     2101 gcaatggtgc aatggacagt ggcaggccag aggcctaccc cacgacgaaa gcgccgcgct
     2161 tgctctgggc ggtggtctgc gggtaaactc ataaagctcg cctgatttgt cccgcccccc
     2221 gggtcccgcc tcattgtatg cgctctccgc ccctagccac gctagtcttc ttgccgattt
     2281 tgatcggtgg catcgccctc gcgccgatgc gggtatgggg agacgacggc acacggagtg
     2341 tgcctagtac actgagcttt gagcttgacg tgcagccgat tctgacg
//
LOCUS       DASUGB010000640         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_350220, whole genome shotgun sequence.
ACCESSION   DASUGB010000640 DASUGB010000000
VERSION     DASUGB010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350220"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFV87_12235"
     CDS             <1..819
                     /locus_tag="VFV87_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX5104576.1"
                     /translation="GLTTGIKTERLYPVSRMVSTITGLTVQRNKAIVGRNAFAHEAGI
                     HQDGMLKERRTYEIMQPEDVGFQRTDLVLGKHSGRAALADRAKALGYTLSGDQLQTVF
                     DEFKKLADRKKDIYDGDIIALIEQRIHGAPAEEEWTLVSWEVTSASGTKPRAKLTLRR
                     GSEEFTEEVAAGDGPIDAAFLATEKITGIKLKCIDYQVRSATLGHDALGEVNLSVQHG
                     RETFRGRGVSTDTVESTVQAILSAVNRVALTQGSGAGVKPTQIAAGEPAGSAAT"
     gene            915..1106
                     /locus_tag="VFV87_12240"
     CDS             915..1106
                     /locus_tag="VFV87_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5104577.1"
                     /translation="MQTVKYVYWQEDGAWLGYLEEYPDYWTQGETLDDLVAHLKDLFL
                     DLSSGQIPAARRVGELVIS"
     gene            1103..1303
                     /locus_tag="VFV87_12245"
     CDS             1103..1303
                     /locus_tag="VFV87_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5104578.1"
                     /translation="MKPDDLIKTIEGFGCRLIRHGGKHDWYQNPTTGVAQAVPRHREI
                     KERLAQRIIRMLSNPDADADQG"
     gene            complement(1304..>2299)
                     /locus_tag="VFV87_12250"
     CDS             complement(1304..>2299)
                     /locus_tag="VFV87_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX5104579.1"
                     /translation="DDVIDVDPTCRKLEELTTEILGKEAALFMPSGTMTNQVGVRIHC
                     KPGDEFLCEAGCHIYNYEQGAFAQLSGVVARTIEGQAGVLTVEQLRGTIRPENDHLVR
                     TRLVCLENTHNRAGGKVQPYDDVVAICDWAHEAGLRTHLDGARLWNATAASGISEAEW
                     SKHFDTVSVCFSKGLGAPVGSALAGTKDAIKEARRHRKLFGGAMRQDGIIAAGALYAL
                     QHNRQRLVEDHVNAKHLAEAVRESAGLSLLYGDVESNIVIFRVEPQLGSAAELVAELK
                     TQGLWCLAIGPQQVRLVTHLDVSESQCRQAATILRETASRLASGKKSAKELEPAY"
BASE COUNT          421 a          750 c          741 g          387 t
ORIGIN      
        1 ggcctgacca cgggcatcaa gaccgagcgg ctctacccgg tcagccgcat ggtttcgacg
       61 atcaccggcc tgacggtcca gcggaacaag gcgatcgtcg gccgcaatgc cttcgcccac
      121 gaggcaggaa tccaccagga cggcatgctc aaggagcgcc gcacctatga gatcatgcag
      181 ccggaggacg tcggctttca gcggaccgac ctggtgctgg gcaagcacag cggccgggcg
      241 gcattggccg atcgggccaa ggccctgggc tacacgctga gtggcgacca acttcagacc
      301 gtcttcgacg agttcaaaaa gctggccgat cgcaagaagg acatctacga cggcgacatc
      361 atcgccctga tcgagcagcg aatccacggc gcgccggccg aggaggaatg gacgctcgtc
      421 tcgtgggaag tgaccagcgc cagcggcacg aagccgcggg ccaagctcac gctccgccgc
      481 ggcagcgagg aattcacgga ggaagtggca gccggcgacg gcccgatcga cgcggcgttc
      541 ctggctaccg agaagatcac cggcatcaag ctcaagtgca tcgattacca ggtccgcagc
      601 gccacgctgg gtcacgatgc cctgggcgaa gtcaacctct cggtccagca cggccgcgaa
      661 accttccgcg gccgcggcgt ttcgaccgac acggtcgaat cgaccgtcca ggcgatcctc
      721 agcgccgtca accgcgttgc tctcacccaa ggctcgggcg ccggcgtcaa gccgacgcag
      781 atcgcagcgg gcgaacctgc cggatcggct gcgacgtagc ggaatccgat tacgctgttc
      841 ttagccggga ccaagcctgg tcccggcgtg ctgcgtcgga tcgtatgatt gcactggact
      901 tccaggagcg aagtatgcaa acggtcaaat acgtctattg gcaagaggac ggcgcctggc
      961 ttggctatct ggaagagtat ccagactatt ggactcaggg cgaaacgctc gacgacctcg
     1021 tcgcccacct caaggacctc tttctcgacc tgagcagcgg gcagattccg gccgcgcgcc
     1081 gcgtcggtga attggtcatc tcatgaagcc cgacgatctg atcaagacga tcgaaggctt
     1141 cggctgccgg ctgattcgac acggcggcaa acacgactgg tatcagaatc ccacaacggg
     1201 cgttgcgcaa gccgtcccgc ggcatcgcga aatcaaagag cgacttgccc agcggatcat
     1261 tcggatgctc agcaatcccg acgcggacgc agaccagggc tagctagtac gccggctcca
     1321 gttccttggc cgacttcttc ccgctggcca gccggctggc ggtttcgcgg aggatcgtgg
     1381 cggcctggcg gcattgggat tcggagacgt cgaggtgcgt gaccaggcgc acttgctgcg
     1441 ggccgatggc gaggcaccag aggccttgcg tttttagctc agcgaccagt tccgcagcgg
     1501 agccgagctg gggctcgacg cggaagatga cgatgttcga ttcgacgtcg ccatacagca
     1561 atgaaagccc ggcggactcg cggactgcct cggcgagatg ttttgcatta acgtgatctt
     1621 caaccagccg ctggcggttg tgttgcaagg catacagcgc accggcagcg atgatgccgt
     1681 cttgccgcat cgcgccgccg aagagcttgc gatgccggcg ggcttcttta atcgcgtcct
     1741 tcgtgccggc gagcgccgag ccgaccggcg cgccgagccc tttgctgaag cagacactga
     1801 ccgtgtcgaa gtgcttggac cactccgcct cggagatgcc gctggccgcg gtcgcgttcc
     1861 ataatcgcgc cccgtccagg tgcgttcgca ggcccgcttc gtgggcccag tcgcaaatcg
     1921 cgaccacgtc gtcatacggc tgcaccttgc cgccggcccg gttgtgcgtg ttctccaggc
     1981 acaccagccg cgtgcggacg aggtggtcgt tctccgggcg gatcgtgccg cggagctgct
     2041 cgaccgtgag cacgcccgcc tggccttcga tcgtgcgggc cacgacgccg ctgagctggg
     2101 cgaaggcccc ctgctcgtag ttgtagatgt ggcagccggc ctcgcagagg aactcgtcgc
     2161 ccggcttgca gtgaatccgc acgccgacct ggttggtcat cgttcccgac ggcatgaaga
     2221 gggccgcctc cttgcccaga atctccgtcg tcagctcctc cagcttgcgg caggtcggat
     2281 cgacgtcgat cacgtcgtc
//
LOCUS       DASUGB010000641         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1877378, whole genome shotgun sequence.
ACCESSION   DASUGB010000641 DASUGB010000000
VERSION     DASUGB010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877378"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV87_12255"
     CDS             complement(<1..188)
                     /locus_tag="VFV87_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104580.1"
                     /translation="MKCQGNRLSRRNVLTIGAIGGLGLTLADFLQMREARADQKHYDF
                     LDAKAQSVIHIFLPGGMA"
     gene            369..536
                     /locus_tag="VFV87_12260"
     CDS             369..536
                     /locus_tag="VFV87_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104581.1"
                     /translation="MAKIAGVVTVVKLRNGDWQEEASLMLAINKKFGQPIEASLNMIL
                     SGGFNYSVVTL"
     gene            complement(526..720)
                     /locus_tag="VFV87_12265"
     CDS             complement(526..720)
                     /locus_tag="VFV87_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEX5104582.1"
                     /translation="MLVLSRKETERIRLGDSIVVTVVRVAGDKVRLGIEAPPDVLVLR
                     EELKPNEQASLHPPSVTPTM"
     gene            complement(825..1121)
                     /locus_tag="VFV87_12270"
     CDS             complement(825..1121)
                     /locus_tag="VFV87_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104583.1"
                     /translation="MSALPLQLDNLNDLRTYVQQTICDQNELEPGAFQVTERILVRGR
                     KPCGIFFCLHGPRSVKLTAIWESDRNTILFYSSSGERVLKTQLIQAPVLSAAAA"
     gene            complement(1354..1986)
                     /locus_tag="VFV87_12275"
     CDS             complement(1354..1986)
                     /locus_tag="VFV87_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5104584.1"
                     /translation="MTTLDAKADCYSAGERGAMGWWQDLRARWLGPLLAAMGAVGIRP
                     DHVTAASLAAGLAFCPLWLWPDGPAWAKPAALVALLLHVLLDGLDGPLARHLGTASSR
                     GSFTDSLADQIVVTATTLALMAAPQPGVLTWIGGTYIFLYATVVGFAMVRNALGNPYT
                     WLVRPRLWVYGWIAVDLALLPGWIDYVIGAFTVVLALKVATGFVALRRML"
BASE COUNT          432 a          589 c          620 g          365 t
ORIGIN      
        1 tgggccatgc cgcccggcag gaagatgtga atgacgcttt gggccttggc gtccaggaag
       61 tcgtagtgct tctggtcggc gcgggcttcc cgcatctgca ggaagtcggc cagcgtcagc
      121 cccaagccgc cgatcgcccc gatggtcagg acatttcgtc ggctcaggcg gtttccctgg
      181 catttcatag cgagaatctc catgtggtaa ccaagcaggt tgggcgattc ggtcgcccgt
      241 ggatcctgcg ggagtggttc gcgagcgctg caagtcacgc gccgcggggt gtaaggtggg
      301 atcgttaggt gggagaggac ggcgggtagc cgtgaccaag cgcaatcaca gtacccggcc
      361 taagctaaat ggcgaagata gccggtgtgg ttacggttgt caaactccgc aacggcgact
      421 ggcaagaaga ggccagcttg atgctggcca ttaataagaa gtttggccag ccgattgagg
      481 ccagcctgaa tatgatacta tcaggtggat ttaattactc tgttgtcaca ttgtaggagt
      541 cacagaaggc gggtgtaaac tcgcctgctc gttcggcttg agctcttccc taagaaccag
      601 tacgtccggg ggggcttcaa ttcccagtcg gaccttgtcg cccgcgaccc gcaccaccgt
      661 aacgactatg gaatcgccga gccggattcg ttcggtttct ttgcgtgaaa ggaccaacat
      721 gccaggaacc tccgtgtatg cactcaagtt gttggatgaa tcgttcttgc ccgaagtggt
      781 tgtctgactg acggttgcgg caatttttcc ctctgactgc aatactacgc agcggcggct
      841 gagaggactg gcgcctggat aagctgcgtc ttgagcaccc gctcgcccga cgagctgtag
      901 aacaagatcg tgttgcggtc cgattcccaa atcgccgtca atttgacgct gcggggtccg
      961 tgcaggcaga aaaagatgcc gcacggcttt cggccgcgga cgaggattcg ctcggtcact
     1021 tggaaggcgc cgggctcaag ttcattctga tcgcaaatgg tttgttggac gtaagttcgc
     1081 aggtcgttca ggttgtcgag ctggagaggt agtgccgaca ttgggggcgc agactcctcg
     1141 cggatgaatt gaagtgggcg gggaagaaac tgccagggag taagaatggg cagtttgggg
     1201 tgagtctcag cagaagtaac tatcgtctga tcgcggaggc agatttaaat gggaaggcta
     1261 cgaaatatga agagcccagg agaaatggtg ctgagtttgc ggggcaatgc ggtcgcgacg
     1321 cagacaccaa cccgacgcgt aaccgaggga cgcttagagc atccgccgca gcgcgacaaa
     1381 gcccgtcgcc actttcagcg cgagtacgac cgtgaacgcg ccgatgacat agtcgatcca
     1441 gccgggcaag agcgcgagat cgacggcaat ccagccgtag acccacaatc gcggccggac
     1501 gagccacgtg tagggattgc ccagcgcatt gcgaaccatc gcgaagccga ccaccgtggc
     1561 atacaggaag atgtacgtcc cgccgatcca cgtcagcact cccggctgcg gagcggccat
     1621 cagcgccaac gttgtcgcgg tcacaacgat ctgatcggcc agcgaatcgg tgaacgatcc
     1681 gcggctggag gccgttccca aatgccgcgc cagcggacca tccagcccat ccagcaaaac
     1741 gtgcaacagc aacgccacaa gcgccgccgg cttggcccaa gccggaccat ccggccacag
     1801 ccacagcggg caaaacgcca accccgccgc cagcgacgct gccgtcacat gatcgggacg
     1861 gatccccacg gcccccatcg ccgccaacag cggccccagc caccgcgctc gcaaatcctg
     1921 ccaccacccc atcgccccgc gctcgcccgc ggaatagcaa tcggctttgg catcgagcgt
     1981 ggtcatcgtt taacccgcag ccgata
//
LOCUS       DASUGB010000642         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1740194, whole genome shotgun sequence.
ACCESSION   DASUGB010000642 DASUGB010000000
VERSION     DASUGB010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740194"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFV87_12280"
     CDS             <1..224
                     /locus_tag="VFV87_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104585.1"
                     /translation="GTLLTGPGSWAYDVMAAQQMRGAILQRQYAIAAQKAQEDAAQKA
                     ARLATHTQRRISEQYRCQRFREFLAAAAQ"
     gene            complement(313..1158)
                     /locus_tag="VFV87_12285"
     CDS             complement(313..1158)
                     /locus_tag="VFV87_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX5104586.1"
                     /translation="MSPEKSAPPHILMCPPEHYGIEYEINPWMSRSRQADREIAYQQW
                     QGLTEILTQLGAKLSFLEPRQGLPDLVFTANAALIYGQRAILSHFRHQQRQGEEPLCA
                     AWFQSHGFVVERLPEERFFEGAGDALFCGETLIAGYRIRSDVRSQQMVGELTGSRVVP
                     LELIDEHYYHLDTCFCPLAPGVAIWFPGAFDDYGRRALAEVVPELIEVAAEEAHSFAC
                     NAVVVGQHVVTNTGCPNLHRDLASRGYSPIATPLSEFVKAGGSAKCLTLRLDGEEAAA
                     WKGEG"
     gene            1260..2282
                     /locus_tag="VFV87_12290"
     CDS             1260..2282
                     /locus_tag="VFV87_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5104587.1"
                     /translation="MQQPNRRRFLVLAAGGLGAAALGGAVWRRISAPLETTRRTTFAL
                     GTQVSVTARHADPAHANVAIDDALDELAYIEHRLLSIYQPESQISRLNRDGCLCDPSP
                     LVVYVLRHAQALSRQTTGAFDITVQPLWEAFAAAAKRGESPDVSTVAAARRKVDWRQL
                     EISDDRIRFRQPGMKLTLNGIAQGFATDRVLATLRGHGIEHALINVGEHAPLGESAAG
                     DPWTVGIQHPRNEDAYVALTDLDGRCLATSGDYATTFSPDRASNHIFDPRTGYSPTEL
                     ASVSVLAPSAMQADALSTALFVVGPDRGLELIRRTPNTDAFFVLKNGSAIATPGFPLV
                     EEGSVS"
     gene            2282..3655
                     /locus_tag="VFV87_12295"
     CDS             2282..3655
                     /locus_tag="VFV87_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX5104588.1"
                     /translation="MQIFFRCLCAAAFFAASAASVAAATIEFLNGTTIQCTVLAKDDT
                     SVTIETVTSGAKSKRTIPLTNIHKVTINTKEYVINERPAAGSSKTSSPKRANPKGSSK
                     SSGASLDDAPDQPAASTKELRSKAEIDALIDELGRTPPDWFETTPLNYPQSLDLSWPD
                     KPPGGWDNQKNVGQYVWDIINHNPSKWREGVRLMHHLLTLHKDDPAKRTKVMNELGRM
                     YHNLHQDYARAAFWWRQAGVDKANPPRGTSVHLAECYWRLGNKQMAVDMLRKMNSIPY
                     DGIKLWADMGETDKALSIAEVALKSPGAQKHFALLYAADACRVGGRTNDALKYYQQLL
                     ALSATGQQKGQIERCQKRAAATVEAIKLYDLSDVKKVADGSYQDEAIGYEGPVRVQVV
                     VKSGRIESVNVTQHREKQFYSAMTDTPAKIIAKQSVKGVDATSSATITSEAIINATAK
                     ALAKGAK"
     gene            3810..>4465
                     /locus_tag="VFV87_12300"
     CDS             3810..>4465
                     /locus_tag="VFV87_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HEX5104589.1"
                     /translation="MRLDIFLPALRKPKPGQPLRVLPLTELAQRVLPASWFSNPATKQ
                     RGLVRRALRWLGPTWLSSPVRRLSQALCFALFLWLFFFVCYPYTAQPGKIWPGWQPQA
                     IAEDGTITLSRAEPADLPPRRGQTIYSDAGPLAVATATSSELTLHPAAGFPPEKLEEM
                     RFALGGPWTLSEQPPGAWPSHYTDSLRGKELIHAESILIIDPLVAISTAIASRTWVWS
                     L"
BASE COUNT          862 a         1511 c         1376 g          716 t
ORIGIN      
        1 gcgggacgtt gctcaccggc cccggctcat gggcctatga cgtgatggcc gcccagcaga
       61 tgcgcggcgc gatcctgcag cggcaatatg cgatcgccgc gcaaaaggcc caggaagacg
      121 cggcccagaa agccgcccgc ctcgccaccc acacgcagcg gcggatcagc gagcaatacc
      181 gctgccagcg gttccgggaa ttcctggcgg cagcggctca ataggccatc gcaccgcttg
      241 tcattcgcct ccatgcggct agcccgcatg gaggcagttg gtgaacgagc ggcgaaagcc
      301 ctccccccgg ctctaccctt cccccttcca cgccgccgct tcttcgccgt ccagccgcaa
      361 cgtcagacat tttgcactgc cgcccgcttt gacgaactcg gagagcggcg ttgcgatcgg
      421 cgaatagcct cggctggcaa ggtcgcggtg caaattgggg cagccggtat tggtaacgac
      481 gtgctggccg acaacaaccg cattgcaggc gaacgagtgg gcctcctcgg cggccacctc
      541 gatcagttcc ggcacaactt ccgccaacgc ccgccggccg taatcgtcaa acgctccggg
      601 aaaccagatc gccacgcccg gcgccagcgg gcaaaagcac gtatcgaggt ggtaataatg
      661 ctcgtcgatc agttccagcg gcacgacgcg cgacccggtc agctcgccca ccatttgctg
      721 gctgcgcacg tcgctgcgaa tgcgatagcc ggcaatcagc gtctcgccgc aaaacagggc
      781 gtcgcccgct ccttcgaaga accgctcctc cggcaggcgc tcgacgacga agccgtgtga
      841 ctgaaaccac gcggcacaca gcggttcttc accctgacgc tgctggtggc ggaagtgcga
      901 aagaatcgcc cgctggccgt aaatgagcgc ggcattggca gtgaagacca ggtcgggcag
      961 gccctgccgc ggctccagaa acgagagttt cgcgccaagc tgagtcagaa tctcagtaag
     1021 cccctgccac tgttgatatg caatctcacg atccgcctgc cgcgaccggc tcatccaggg
     1081 gttgatctcg tattcaatgc catagtgctc gggcgggcac atgagaatgt gcggcggcgc
     1141 ggatttttct ggcgacatgc ggcgctccgg taacatttct aatcctgggc gcgacttcct
     1201 tgcgaggcaa gggcggtcgc gaatagactt ctctggggca gagcacgtcg gaggcagcca
     1261 tgcagcaacc gaatcgacgc agattcctcg ttctcgcggc cggggggctg ggcgccgcag
     1321 cgctgggcgg cgcggtttgg cgacgcatat ccgccccgct ggaaacgacc cgtcgcacga
     1381 catttgcgct gggtacgcag gtatccgtca ccgcccgcca cgcggatccg gcacacgcca
     1441 atgtcgcgat tgacgacgcc ctggacgaac tggcctatat cgagcaccgg ctgctgagca
     1501 tctatcagcc ggagagccaa atcagccgat tgaaccgcga cggctgcttg tgtgatccct
     1561 cgccgcttgt cgtgtatgtg ctgcggcacg cgcaggcgct gtcgcgccag acgaccggcg
     1621 ccttcgacat taccgttcag ccgctctggg aagctttcgc tgccgctgcg aagcggggtg
     1681 aatcgcccga cgtgtccacc gtcgccgccg cgcggcggaa agtcgattgg cgacagctcg
     1741 aaatcagcga cgaccgcatt cgcttccgtc agccgggcat gaagctcacc ctcaacggca
     1801 tcgcgcaagg cttcgcgacc gaccgcgtgc tcgccaccct gcggggtcac ggcatcgagc
     1861 acgccctgat caacgtcggc gagcacgccc cgctcggcga gtcggcggcc ggcgatccgt
     1921 ggacggtcgg cattcagcac ccgcggaacg aggatgccta cgtcgcgctg accgatctgg
     1981 acggccgctg cctggccacc agcggcgact atgccacgac gttttcgccc gatcgcgcga
     2041 gcaatcacat tttcgacccc cgcaccggct attcgccgac ggagctcgcc agcgtcagcg
     2101 tgctggcgcc ctccgcgatg caagccgacg ccctctcgac ggccctcttc gttgtgggac
     2161 cggaccgtgg cctggagctg atccggcgca cgccgaacac ggatgccttc ttcgtcctca
     2221 agaacggcag cgcgatcgcg acgcccggct tcccgctggt ggaagaaggg agcgtgtcct
     2281 gatgcagatt tttttccgct gcctgtgtgc cgcggcgttc ttcgccgcat cggccgcatc
     2341 ggttgccgcc gccacgatcg aattcctcaa cggcacaacg atccaatgca ccgtcctggc
     2401 aaaggacgac acgagcgtca cgatcgaaac ggtgaccagt ggcgccaaga gcaagcgcac
     2461 catcccgctc acgaatattc acaaggtcac gatcaacacc aaggaatacg tgatcaacga
     2521 gcggccagcc gctggatcgt ccaagaccag cagcccgaag agggcgaatc cgaaaggctc
     2581 cagcaaatcc agcggcgcca gcctggatga tgcccccgac cagccagccg ccagcacaaa
     2641 agaactgcga tcgaaggcgg aaatcgatgc cctgatcgac gagctcggtc gcacaccacc
     2701 cgactggttc gagaccacgc ctctcaatta tccgcaatcg ctcgacctgt cctggcccga
     2761 caagccgccg ggcggctggg acaaccagaa gaacgtcggc caatacgtat gggacatcat
     2821 caaccacaat cccagcaagt ggcgcgaagg ggtccgcctc atgcaccatt tgctgacgct
     2881 ccacaaggac gatccggcga agcggaccaa ggtcatgaac gagctggggc ggatgtatca
     2941 caacctgcac caggactacg cccgggccgc gttctggtgg cggcaagccg gcgtcgataa
     3001 ggcgaatccc ccgcgcggca cgtcggtgca cctcgccgag tgctactggc gccttggcaa
     3061 caagcagatg gccgtcgata tgctccgcaa gatgaattcg attccctacg acggcatcaa
     3121 gctctgggcc gacatgggcg aaaccgacaa ggccctctcg atcgccgaag tggccttgaa
     3181 aagcccgggc gcacagaagc attttgccct gctctatgcg gccgatgcct gccgcgtcgg
     3241 cggccggacc aacgacgcgc tcaagtacta ccagcagctc ctcgcgctct ctgcgaccgg
     3301 ccagcaaaag gggcagatcg aacgctgcca gaaacgggcc gccgcaacag tggaagcgat
     3361 caagctttat gacctctcgg atgtgaagaa ggtcgccgac ggcagctacc aggatgaagc
     3421 gatcggctac gaaggcccgg tgcgcgtcca ggtcgtggta aagtccgggc ggatcgaatc
     3481 ggtcaacgtc acgcagcacc gggagaagca gttctattcg gcgatgaccg acacgcccgc
     3541 caagatcatc gccaagcaaa gcgtcaaggg cgtcgatgcc accagcagcg ccacgatcac
     3601 gagcgaggcg atcatcaacg ccacggcgaa ggcgctggcg aagggagcga agtgaattcg
     3661 cgccgacgtc tcgtgcccaa gctctgcttg ggcacgcgcg ttctcgacgc tcctgcttcg
     3721 agaacgcgaa cgggaagctg gagcttccag tcgtgggttc ccaagcggag cttgggaacc
     3781 aggtcgataa gagagacgga ggaatccgca tgcgacttga tatcttcctc cccgccctcc
     3841 gcaagcccaa gccgggacag ccgctgcgtg tcctgccgct gacggagctt gcgcagcgcg
     3901 tgctgccggc aagctggttc tcgaacccgg cgaccaaaca gcggggcctg gtgcgccgcg
     3961 cgctgcgttg gctcggaccg acgtggctca gttcgccggt gcggcgattg tcgcaggcgc
     4021 tttgctttgc cctcttcctg tggctgttct ttttcgtttg ctacccgtac acggcccagc
     4081 cggggaagat ctggcctggc tggcagccgc aggcgattgc ggaggacggc acgatcacgc
     4141 tgtcgcgggc tgaaccggcc gatttgccgc cccgccgcgg gcagacgatt tattccgacg
     4201 ccgggccgct cgccgtcgcc acagcgacgt ccagcgaatt gactctccac ccggccgcgg
     4261 gcttcccgcc ggaaaagctc gaggaaatgc ggttcgctct cggcgggcct tggactcttt
     4321 cggagcagcc gccgggggcc tggccgtcgc actacactga cagcctgcga ggcaaggaac
     4381 tcatccacgc ggaaagcatc ctgatcatcg acccgctggt cgccatcagt acggccatcg
     4441 ccagccgcac ctgggtctgg tcgct
//
LOCUS       DASUGB010000643         5220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_767147, whole genome shotgun sequence.
ACCESSION   DASUGB010000643 DASUGB010000000
VERSION     DASUGB010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767147"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFV87_12305"
     CDS             complement(<1..1252)
                     /locus_tag="VFV87_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX5104590.1"
                     /translation="MKTLAPSASRKSRRNLRQANELHRQGRTRKMFFESLEPRQMLTY
                     TPGEIAALDQQFPGNYLPPQHAQLPTNGSLLSTAATGNRTTLALNYLSANAASFGLTA
                     NDFTHSVVTDNYISNGIRHIYVQQTFNGLPVVDAKASVHILQSTGQIVSASANFVPNL
                     PHPTNPAAPTPEIAAQDAFGYFASALGTPLSQPITLNSQSPDAARLTVITAPDYSAEP
                     INMQLHYVPRPDGSVALGWRINFFAVDHKHWWDATVGAQWNTETGKVLNLTDWLWGAS
                     YNVFARPTQDPFDGPRTIVVDPHDAAVASPFGWHDANFVAGPEFLDTRGNNVSAQEDQ
                     NADDQGGNRPTSPTLDFDPPLVLPSSPFTYTDATTTNVFYWVNLVHDVTFNHGFDEAS
                     GNYQQVNRTGQGRGNDRIIADVQDP"
     gene            complement(1496..2836)
                     /locus_tag="VFV87_12310"
     CDS             complement(1496..2836)
                     /locus_tag="VFV87_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104591.1"
                     /translation="MAILVGGFFASVAHSDERIPADAALRWWKGNLHTHSLWSDGDDF
                     PEMIAEWYRTRDYQFLALTDHNVLSQGIRWMKADDIAKRGGEDVLRKYRDRFGPAWVE
                     MRGDGQSSSLEVRLKPLDEFRALVEERGKFLLIPAEEISDKAEGVPVHINASNLRDPL
                     NPVGGATVRETIENNLRSIDEQAKTAGREILAHLNHPNFGWAITAEDLAYVVTDRFFE
                     VYNGHPVVNQPGDQHRPSVERLWDIANTIRLAKLDAPPLYGVATDDSHAYHGKPNGAR
                     PGRGWIMARSTHLTPEYLIRAIKAGDFYASSGVTLSDVRYDAAAKTLEISIEPDGDAA
                     YQTQFIGTLRGFDDSSEPTLDDDGQPLKDKSGKPLRASRKYSDEIGQVLATHTGLSPR
                     YTLSGKELYVRAVVTSNQSPRDPSFAGQKAQAWTQPVGWESPVVKPSQDVKVER"
     gene            complement(3073..3327)
                     /locus_tag="VFV87_12315"
     CDS             complement(3073..3327)
                     /locus_tag="VFV87_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane or secreted protein"
                     /protein_id="HEX5104592.1"
                     /translation="MNARRLIVALAIACVGAGGCASNGQFTLFNPGTVNQQRLRATIH
                     DPYSDQDLAPEVVGGRPRDYLQQLPEPVRNRLYVDSGYVR"
     gene            3501..3887
                     /locus_tag="VFV87_12320"
     CDS             3501..3887
                     /locus_tag="VFV87_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104593.1"
                     /translation="MCSLRKALLLGLLVWLVPFAVAFCVFPLKTSWRSLFESIMPVTL
                     AAVVTVCALLYFRRATAASVREGLLLGAIWLAISVAIDLPLMLSPPISMPPLEYAADI
                     GLTYLMIPVITVGIAVAQTNRAITGR"
     gene            4028..>5220
                     /locus_tag="VFV87_12325"
     CDS             4028..>5220
                     /locus_tag="VFV87_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5104594.1"
                     /translation="MTNFLRIVRLTLRRRLTFIGAILCSLGVAVLWGANLGLAKPIVE
                     LIFADLPPHAWVDAQVVDAQEKVAVTRARLEDLEQRIRNAAQPDRAPLVAQQEKLQQR
                     LELEEKQLNGAKWLQPLAKRYVPNSTFGALAMCIGLLVLATLLKDAMLVGNLMLVERL
                     TQLAMFDLRKQLFRRTLQMDLAHFGREHSGQTMHHLTSDLQCAFQGVSVLCGRMILEP
                     LKALACLIGAALICWKLLVLSLIVAPLAGFLVFRLTQSLKRANRRAMEEMGLLYTLLS
                     ETITNIQAVKAFGMERHERRKFHVRGKSYFRKAMRIVFYNSLGRAGTEFMGLTIICVA
                     FIAGTYLVANPQTKILGITIIDRPLSLGSLLAFYALLAGIGDPSRKMSEVLNQFQRGV
                     VAADRV"
BASE COUNT          963 a         1523 c         1668 g         1066 t
ORIGIN      
        1 tgggatcctg aacgtcggcg ataatccggt cgttgccgcg tccttgacca gtacggttga
       61 cttgctgata gttgcccgag gcctcgtcga atccgtggtt gaacgtgacg tcgtgtacga
      121 gattgaccca atagaagacg ttggttgtcg ttgcgtccgt gtatgtgaac ggggacgaag
      181 gcaagacgag cggcgggtca aaatccaagg tcgggctggt tggacgattc ccaccctgat
      241 cgtcggcatt ctgatcttcc tgcgccgaaa cgttgttgcc gcgggtgtcg agaaactcgg
      301 gtccggcaac gaaattcgca tcgtgccagc caaagggcga agcgacggcg gcatcgtgtg
      361 gatcaacaac gatcgtgcgc ggcccgtcaa acggatcctg tgtgggccgc gcaaaaacgt
      421 tgtacgatgc gccccagagc cagtccgtca gattcagcac tttgccggtc tcggtattcc
      481 attgcgcgcc gaccgtcgca tcccaccagt gcttgtgatc gacggcaaag aagttaatcc
      541 tccagcccag cgccacgctg ccatctggcc gcgggacgta gtgcaattgc atgttgatcg
      601 gctcggccga gtagtcgggc gccgtaatga ccgtgagccg tgccgcatcg ggcgactgtg
      661 aattgagcgt gatcggttgt gacagcggag tgccaagggc ggaagcaaag tagccaaacg
      721 cgtcttgcgc tgcgatctca ggcgtgggcg ccgctgggtt tgtcgggtgg gggagattgg
      781 ggacaaaatt ggcactggcg gaaacgatct gtcctgtgct ctgcaggata tgaacgctgg
      841 ccttggcatc cacgaccggc aggccgttga acgtttgctg cacataaatg tgacgaatgc
      901 cgttggaaat gtagttgtcc gtaacgacag agtgcgtaaa gtcgttcgcg gttaggccaa
      961 atgaagccgc gttggcagac aaatagttga gcgcgagagt ggttcgattg ccggttgccg
     1021 cggtactgag caaggatccg ttggtgggta attgcgcgtg ttgcggcgga aggtaattgc
     1081 ccgggaactg ctgatcgagc gcggcgatct cgcccggcgt gtaagtcaac atctgccgcg
     1141 gctcaagcga ttcaaagaac atcttgcggg tacggccctg ccgatgcaac tcattggcct
     1201 gccggagatt gcgacgagac ttccgcgatg cggagggagc gagcgtcttc acgagttcaa
     1261 ccctgccaac ggcgaataat gacgagtgga agcgcagcca tcctgccggg ccgcgtgcgc
     1321 aatagggcct caccgaatac tggcgtagtc taattgccgc ctatcggaat tgccacaatt
     1381 ttgcaggaag ttagggactt ttcacagctt tacgacagtg agagtcgctg attcccctgg
     1441 cggcggaccg ggcgtaggcg gaccccgacc cgcctcatag acgcatcttg gccaactatc
     1501 gttccacttt cacgtcttgg gacggcttta ccacgggcga ttcccaccca acgggttgcg
     1561 tccaagcctg ggccttctgt cccgcaaacg atgggtcccg gggcgattga ttcgaggtga
     1621 cgaccgctcg cacgtataac tctttgccgc tcagcgtgta gcgcgggctc agccccgtat
     1681 gggtcgccag cacttgaccg atttcatccg aatatttgcg gcttgcgcgg agtggtttgc
     1741 cgctcttgtc cttcaggggc tggccgtcgt cgtccaaggt cggctcgctg gaatcgtcga
     1801 acccgcgcaa cgtgccaatg aattgcgtct gatacgccgc atcgccgtcc ggttcgatcg
     1861 atatctccag cgtcttggcc gcggcatcgt agcgaacgtc gctcagcgtc acgccgctgg
     1921 aagcgtagaa atcgccggcc ttgatcgccc gaatgaggta ctcgggcgtt aagtgcgttg
     1981 aacgcgccat gatccagccg cgccccggcc gcgcgccgtt cggcttgccg tgataagcat
     2041 ggctgtcgtc ggtggcgacg ccgtagaggg gcggcgcatc gagttttgcc agccggatcg
     2101 tattggcgat gtcccacaaa cgctcgacgc tcggccggtg ctggtcgccc ggctgattga
     2161 cgactggatg gccgttatag acctcgaaga accggtcggt cacgacgtag gccagatctt
     2221 ccgcggtgat cgcccagccg aagttcggat ggttcagatg ggccaggatt tcgcggccgg
     2281 cagttttggc ctgctcgtcg atgctccgca ggttgttctc gatggtttcg cggacggtgg
     2341 ctcctccgac ggggttcagc gggtcgcgca ggttcgaggc gttgatgtgg accggcacgc
     2401 cttccgcctt gtcgctgatt tcttccgcgg gaatcagcag gaacttgccg cgctcctcga
     2461 ccagggcgcg gaactcgtca agcggcttga gtcggacttc cagcgaactg ctttgcccat
     2521 cgccgcgcat ctcgacccag gcggggccga agcggtcgcg atatttcctc agcacgtcct
     2581 cgccgccgcg cttggcgata tcatcggctt tcatccagcg gataccctgg ctcaggacgt
     2641 tgtgatcggt gagggccagg aactgataat cgcgcgtccg ataccactcg gcgatcattt
     2701 ccggaaagtc atcgccatcg ctccacagcg aatgcgtatg caaatttccc ttccaccacc
     2761 gcagcgcggc gtctgccgga attcgctcat cggagtgagc gacgctggca aaaaagccgc
     2821 ccacgaggat ggccaataaa gcaaacgttg cgccgctaat tcgatcgccg tgcgcacacc
     2881 gaaccgctgc ggattttggg taaatcgctg gcacttgatc gctccccgcg aagaaaaatc
     2941 ttccgcccgc tggcaagccg ccagtcggcg cagcattacc ctactcggcg gctgagtggg
     3001 agtaaaccaa gaggggctga gattcgactc tgccggtagc gggttttggc agtcaagaat
     3061 gcctgcccaa cgctaccgca cgtaaccgct atcgacgtag aggcggttgc ggacgggctc
     3121 gggtagctgc tgcaggtaat cgcggggccg gccgccgacg acttccggcg ccaggtcctg
     3181 atcggagtac ggatcgtgaa tcgtcgcccg caggcgctgc tggttgaccg tcccaggatt
     3241 gaagagcgtg aactgcccat tgctcgcgca gccgccggct cccacgcatg caatcgccag
     3301 ggcgacgatc agccggcggg cattcatgaa acgatccttg caagtgggcc gcggcaaagg
     3361 gagaatcggg ccgcggattg tacgcaagcg tccggctcgc ggccaatccg aaaaacagaa
     3421 ctgcccgtgc tatcgcaaaa gcgacagcaa cgcctggcta acggtgctgc gcagcggcag
     3481 ggtcacgaat ctgaactgtc atgtgctcgc ttcgtaaggc actcctgctc ggcttgctgg
     3541 tctggctcgt tccgttcgcg gtggcattct gcgtctttcc attgaaaacg tcctggagat
     3601 cgctgttcga gtcgatcatg ccggtaacgc ttgcggctgt cgttacggtg tgtgccttgc
     3661 tctatttccg gcgcgcgaca gcggcctcgg tccgcgaagg gttgctcctg ggggcgatct
     3721 ggctggcgat cagcgtggcg atcgatctgc cgctgatgct cagcccgccg atcagcatgc
     3781 cgcccctgga gtacgccgcc gatattggac tgacctacct gatgattccc gtaatcacgg
     3841 tgggaattgc agtggcgcaa acgaaccgcg cgataactgg acggtaagcg ttgatgcggg
     3901 aaacaagtca tcttggttga tttgccgcgg gtgtgttaca aggtccaccg ccactggcag
     3961 gttcttgctg gcgagatcgc tgctagcgtc atacacgcag ccggcccctt gatgacgtct
     4021 tctgcgcatg accaactttc tccgcatcgt ccggcttacg ctgcggaggc ggttgacgtt
     4081 cattggggcg atcctctgct cgcttggtgt ggccgtgctg tggggggcca atctggggct
     4141 ggccaagccg atcgtcgagc tgatcttcgc cgatttgccg ccgcacgcgt gggttgatgc
     4201 ccaggtcgtc gatgcccagg aaaaagtcgc ggtcacccgg gctcggctgg aggatctcga
     4261 gcagcggatt cgaaacgccg cgcagccgga cagagcaccg ctcgtcgctc agcaagaaaa
     4321 gctccagcag cggctggagc tggaagaaaa acagctcaat ggcgccaaat ggctgcagcc
     4381 gctggccaag cgatacgttc ccaacagcac cttcggggcg ctggccatgt gcattggcct
     4441 cttagtgctg gcgacgctgc ttaaagacgc aatgctggtc ggcaacctga tgctggtcga
     4501 acggctgacg caactggcca tgttcgatct gcggaagcag ctcttccgga ggacgctgca
     4561 gatggacctg gcccattttg gccgggagca ttcgggccaa acgatgcacc acctcaccag
     4621 cgacctgcaa tgcgcgtttc aaggcgtgag cgtgctgtgc gggcggatga tcctggagcc
     4681 gctcaaggcc ctggcgtgcc tgatcggggc tgcgctcatc tgctggaagc tgctcgtgct
     4741 gtcgctgatc gtcgcgccgc tggcgggctt tctcgtcttt cggctgacgc agtcgctgaa
     4801 gcgtgccaac cgccgcgcga tggaggagat ggggctgcta tacaccttgt tgagcgaaac
     4861 gatcaccaac atccaggcgg tcaaggcgtt cggcatggag cggcacgagc ggcggaagtt
     4921 ccacgtgcga ggaaagagct acttccgcaa ggcgatgcgg atcgtgtttt acaactcact
     4981 gggccgggcc ggcacggaat tcatggggct gacaatcatc tgcgtcgcgt tcatcgcggg
     5041 gacgtatctg gtcgccaatc cacagaccaa gatcctggga atcacgatca tcgatcggcc
     5101 gctgagcctc ggttcgctcc tggcctttta tgctctgctg gcggggatcg gcgatccttc
     5161 gcgaaagatg tcggaagtgc tcaaccagtt tcagcggggc gtggtggccg cagaccgcgt
//
LOCUS       DASUGB010000644         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_906287, whole genome shotgun sequence.
ACCESSION   DASUGB010000644 DASUGB010000000
VERSION     DASUGB010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906287"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VFV87_12330"
     CDS             <1..1779
                     /locus_tag="VFV87_12330"
                     /inference="COORDINATES: protein motif:HMM:NF020939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HEX5104595.1"
                     /translation="NAFGMGIDKRDLRFVVHYNIPGSLEAYYQEAGRAGRDGLDSRCL
                     LIYSARDRGTQEWFIENRYPEPEVVEQVYDYLREIDADPIELTLEEIAANLRLQVRSE
                     GVSASEKLLENCGALERLDSRQNRGGVRIDSDLPTLVDLLPRDAKSQRKVVQAVEKVV
                     GELRYERVFINPRQLAQLAGMELAAVNRALKELCKLQSFDYVPPFRGRAVHMLEREKE
                     FDELGIDFDEQLRLKDAEYAKLDRVIQYATSRRCRQLEILDYFGDPSKQKCGTCDNCL
                     ASGHQASGDKASGGQSSTRLASGGRQAPEQPARRGDSPAPNFCLPQVLDAVRIVLSGV
                     ARMKGRYGKQMIARMLVGSKSKEAARFGQLSTFGMLSALNESQAVALIDALLAARLLM
                     QVEETPRRPLVRLTPQGQEVMKGIAEPGERLHLDDSLVAKLLAIQLPTAGGSTPQRNT
                     TDLPIQKAVEVDPLEAANAELAEPAAFDDEPANSPSPTHRPDANHEARPPHYWTWRVL
                     ASGFSAEECQQIRGITADELCDHLLQAAKSGQPVEPRWVLTTEQMATLAKRFGNMPAD
                     RTRTNRAQLPPDVTDRQVQIYLLGRG"
BASE COUNT          393 a          636 c          666 g          343 t
ORIGIN      
        1 aacgccttcg gcatgggaat cgacaagcgc gacctgcggt tcgtcgtcca ctacaacatt
       61 cccggcagcc tggaggcgta ttaccaggag gcggggcggg ccggccgcga cgggctcgat
      121 tcgcgctgcc tgctcatcta ctcggccagg gaccgcggca cgcaggaatg gttcatcgag
      181 aaccgctatc cggagccgga agtcgtcgag caggtctatg actaccttcg cgaaatcgat
      241 gccgacccga tcgagctgac gctggaggaa atcgccgcga acctgcggct ccaagtgcgg
      301 agcgaggggg tgagcgcctc ggaaaagctg ctggagaact gcggggcgct ggagcggctc
      361 gattcgcggc agaaccgcgg cggcgtgcgg atcgacagcg atctgccgac gctggtcgat
      421 ctgttgccca gagatgccaa gtcgcagcgg aaggtcgtcc aggcggtgga gaaggtcgtc
      481 ggcgagttgc gatatgagcg ggtcttcatc aacccgcggc agctcgcgca gcttgccggc
      541 atggagctgg cggcggtcaa tcgggcgctg aaagagctgt gcaagctgca gagcttcgac
      601 tatgtgccgc catttcgcgg ccgggcggtc cacatgctgg agcgcgagaa ggagtttgac
      661 gagctgggga tcgacttcga cgagcagctt cgccttaagg acgcggaata cgccaagctc
      721 gaccgggtga tccaatacgc caccagccgc cgctgccggc agcttgagat tctcgattac
      781 ttcggcgatc cgtcgaagca aaagtgcggg acgtgcgaca actgcctggc gagcgggcat
      841 caggcgagcg gagataaggc gagcgggggc cagtcgagca cccgactggc gagcgggggc
      901 cgtcaggccc ctgagcaacc cgcgcggcgc ggcgactccc ctgcgccgaa cttctgcttg
      961 ccacaagtcc tcgacgccgt ccgcatcgtc ctctccggcg tcgcccgcat gaagggccgg
     1021 tatggcaagc agatgatcgc gcgaatgctc gtcggctcga aatcgaagga agccgcgcgc
     1081 ttcggccaac tcagcacgtt tgggatgctg agcgccttga acgagtcgca agcggtcgca
     1141 ctcatcgacg cgttgcttgc cgcccggctg ctgatgcagg tcgaagagac gccgcgccgc
     1201 ccgctggtcc gcctcacgcc gcaaggccag gaagtcatga aaggcatcgc cgaaccgggc
     1261 gagcggttgc atcttgacga ttcgctggtc gcgaagctcc tggcgattca gcttccgacg
     1321 gcaggtggtt ccactccaca gcgcaataca actgacctac ctattcagaa agcggtggaa
     1381 gtcgatccct tagaagctgc aaacgccgag ctcgccgagc cggctgcttt cgacgatgag
     1441 ccagccaact cgccgtcgcc cacacaccgg ccggacgcaa accacgaagc tcgcccgccg
     1501 cactattgga cctggcgggt cctcgcctcg ggcttctcgg cggaagaatg ccagcaaatc
     1561 cgcgggatca ccgccgacga actgtgcgac cacttgctcc aagccgcaaa gagcggccag
     1621 ccggtggagc cgcgttgggt gctgacgacg gagcagatgg caaccctagc caaacgattc
     1681 ggaaacatgc cagccgatcg cacgcggacg aatcgtgcgc agctccctcc cgacgttacc
     1741 gatcggcagg tgcaaatcta cttgcttggc cgagggtgaa ggaaactgcg gtaagaggtt
     1801 tcacgaatgg gctcccaatt ccgaagccgt cgccgccgtg agaaggagac ctctgagccg
     1861 gaacgtagtt ttttctattt cacgtggtgc gactcctggg gacgaccgcc gcgggtctac
     1921 ttgtgggcta tgtggctgat caatggactc atcgcactcg ccggcgttgg catgttggca
     1981 acgttcgtct tgctgaatct gcctgacgtt tggtccgacc ccctcccctg gctctcag
//
LOCUS       DASUGB010000645         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_906372, whole genome shotgun sequence.
ACCESSION   DASUGB010000645 DASUGB010000000
VERSION     DASUGB010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906372"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFV87_12335"
     CDS             complement(<1..706)
                     /locus_tag="VFV87_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104596.1"
                     /translation="MSTNLYESTPYSHSFTQPQPAPAGGLLPVALVAVGAMLVLGAAG
                     AGIVLCLGGDWMLSDAERERRDLIETIELTWGLPAHEEAAVIAQVNRIFDAHTSGQIT
                     YEQCEGIFTELMKTPHFAILSARDFDEFYVPDADLRGPARRAATRTIERALRGLLREQ
                     ISRDDFYAAMPAIYSFDDRFTEQELDEMSDEEYDELLAEEPDPTPAQIRESLVKLQAL
                     ADHAGIPDEPFHADFAL"
     gene            960..>2277
                     /gene="gltX"
                     /locus_tag="VFV87_12340"
     CDS             960..>2277
                     /gene="gltX"
                     /locus_tag="VFV87_12340"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5104597.1"
                     /translation="MPVRTRFAPSPTGYLHIGGVRTALFNWLFARQHGGQFLLRIDDT
                     DAERNVAEALQPILDGFKWLGLDWDEGPTDDGESSRGPCGSYFQSQRLPQYQAAVKSL
                     LEKGLAYRDFATPEELKAEREAAEKEKRPYLYSRGWMAETDKEAAKFAAEGRSQVVRM
                     RMPRDGQCEFQDLVRGLCVFDWDNEQDHVIQRADGTCLYHLASVVDDYDFKITHVIRA
                     VEHLSNTPRQIFIAQSLGYPLPEYAHLPYVAEPGSPQKLSKRKIGTYLKNAEFKRMYD
                     HGAEIAARIGHVTAAETFNPVIVDFYREVGYLPDAIINYLMLLGWSLDDKTEDLSRAE
                     MIQHFSLDRVTKAPASFDPKKLWAFQDRYMQRLPLKQKVAKMLPYLQKAGLISDPPPC
                     DGGPKLSRIIEASGDRLKVAGDILAYADFFFVSDDKLAHDEKDFAKR"
BASE COUNT          426 a          742 c          724 g          385 t
ORIGIN      
        1 cgagcgcgaa gtcggcgtgg aagggctcgt cggggatgcc cgcgtggtcg gccagggcct
       61 gcagcttgac gagcgactcg cggatttggg ccggcgtcgg gtcgggttcc tcggccagca
      121 gctcgtcgta ctcctcgtcc gacatttcat ccaactcctg ctcggtgaag cggtcgtcga
      181 acgaatagat agccggcatg gcggcgtaga agtcgtcgcg gctgatttgc tcccgcaaga
      241 ggccgcggag ggcccgctcg atcgtccgcg tggcggcccg ccgagcggga ccgcgcaggt
      301 cggcgtctgg cacgtagaac tcatcgaaat cgcgggccga gaggatggcg aagtgcggcg
      361 tcttcatgag ctccgtgaag atgccctcgc actgctcgta ggtgatctgg ccgctcgtgt
      421 gggcgtcgaa aatccggttc acctgcgcga tgaccgcagc ttcctcatgc gccggcaagc
      481 cccaagtcag ctcgatcgtc tcgatcaggt cccgtcgctc gcgctccgca tccgaaagca
      541 tccagtcgcc acccaggcag agcacgatcc ccgcccccgc cgctcccagc accagcatgg
      601 cgcccaccgc cacaagcgcc acgggtagca ggccgccggc cggcgcaggc tgcggctgcg
      661 taaacgagtg cgagtagggg gtggattcgt acaagttcgt ggacatgggc tcactctcct
      721 gcgactgcaa ccggacatcg gcaaggcggc tgaagcgcga atcgcttgcc cggtcgatgc
      781 gaccgttaga atcggccttg ctaataggcc aaacggccgc cgggcaagcc taggtcacgc
      841 ccgacgcgtg gcaacgtccc actcgatcga ttgtgcgtga accagcacgt gccggggctc
      901 agccggtaga atcgtggttc tgaaactcgc aatccgtaat ccgtaatccg caattgccca
      961 tgcccgtccg cacccgcttc gctcccagtc cgaccggtta cttgcatatc ggcggcgtcc
     1021 gcacggcgct gttcaactgg ctctttgccc ggcagcacgg cgggcagttc ctgctgcgga
     1081 tcgacgatac ggacgccgag cggaacgtgg ccgaggcctt gcagccgatt ctggatggct
     1141 tcaagtggct gggcctcgat tgggatgaag gtccgaccga cgacggcgag tccagccgcg
     1201 ggccgtgcgg ctcgtatttc cagtcgcagc gtttgccgca gtatcaggcg gcggtgaaat
     1261 cgctgctgga gaagggcctg gcctaccgcg atttcgcgac gcccgaggaa cttaaggccg
     1321 aacgcgaggc ggccgagaaa gaaaaacggc cgtacctcta cagccgcggc tggatggccg
     1381 agactgacaa agaggcagcg aagttcgccg ccgagggccg ctcgcaggtg gtgcggatga
     1441 ggatgccccg cgacggccag tgcgagtttc aagatctcgt ccggggcctg tgcgtcttcg
     1501 actgggacaa cgagcaggat cacgtcatcc agcgagccga cggcacctgc ctctatcacc
     1561 tggccagcgt cgtcgatgat tacgatttca agatcacgca cgtcatccgc gccgtcgagc
     1621 acctcagcaa cacaccgcgg cagattttca tcgcccagag cctcggctat ccgctgccgg
     1681 aatatgccca cctgccgtat gtcgcagagc cggggagccc gcagaagctg agcaagcgga
     1741 agatcggcac gtacctgaag aacgccgagt tcaagcggat gtacgaccac ggcgcggaga
     1801 tcgcggcgcg gattggccac gtcacggccg cggagacctt caaccccgtt atcgtcgatt
     1861 tctaccgcga agtcggctac ctgccggatg cgatcatcaa ctacctgatg ctcctcggct
     1921 ggtcgctcga tgacaaaacc gaggatctgt cgcgcgcgga gatgatccag cacttttcgc
     1981 tcgaccgcgt gacgaaagcg ccggccagct tcgacccgaa aaagctctgg gccttccagg
     2041 accgctacat gcagcgcctg ccgctcaagc agaaggtcgc caaaatgctg ccctacctgc
     2101 aaaaggccgg cctcatcagc gatccgccgc cgtgcgacgg cggccccaag ctctcgcgga
     2161 tcatcgaagc ctccggcgac cggctgaagg tcgcgggcga cattcttgca tacgcggatt
     2221 tcttttttgt gtcggacgac aagctcgccc atgacgagaa ggactttgcc aagcgcg
//
LOCUS       DASUGB010000646         6997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_627653, whole genome shotgun sequence.
ACCESSION   DASUGB010000646 DASUGB010000000
VERSION     DASUGB010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627653"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..793
                     /locus_tag="VFV87_12345"
     CDS             101..793
                     /locus_tag="VFV87_12345"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX5104598.1"
                     /translation="MRALSRAEVREVDRRAIEQHGMSGLVLMENAGRGCVDTLMSLGC
                     KGPVAVVCGKGNNAGDGFVIARHLEIRGIPVTVILLCAPAELRGDAAANYAILAKGDR
                     PLVDLSGRYSSDSFQAELVGADWIIDALLGTGATGAPRPPMDDAIRLMNASPACRLAV
                     DLPSGLDCDTGSPAEPTIRADHTCTFVAPKIGFASPKAVPYLGEVHVLDIGVPSGVVD
                     QVSGESFLASDL"
     gene            complement(801..1265)
                     /locus_tag="VFV87_12350"
     CDS             complement(801..1265)
                     /locus_tag="VFV87_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5104599.1"
                     /translation="MTVLLANLADLAHQAAIVELLDMYCRDPFGDGRPLSEEARKNLI
                     PGMARHGGALVFLAYEDEKPVGLAICFVGYSTFRGKPLVNIHDIAVRPGSRGQGVGRA
                     LLAAVERHARELGCCKVTLEVRSDNLRAMGAYRRADFEPTEPQTWFWTRKLE"
     gene            complement(1300..2400)
                     /locus_tag="VFV87_12355"
     CDS             complement(1300..2400)
                     /locus_tag="VFV87_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX5104600.1"
                     /translation="MISTKPSRAALPKQQTERIPLQAVADMDITAYDAAAASVSPLLL
                     GQYRPVITPVGMENPAFDPQFAALLDLATRLSRETEADALLVMLEARIEWAELLKRLD
                     GLKVVVAAESGEHLEGAPEAGLATIVLNMPDAPVIEKLTQALLTGVAREILAPGASVV
                     VVYSGFEVDTIDSISYIQLEEHLGRLTARDLRQLETSVPLETLKTVIDLAVEIGREGR
                     EGKAVGTMFVVGDTRKVLQLCQPAGFDPVKGYSRAERDLHDPRVREAIKEVAALDGGF
                     IVNGEAIVEKAAQLVDAPYANLTLSKGLGARHWAGAAISKVTNAIAVVVSQSSGTVRI
                     FQDGEVMLRIEPFHQAMKWKDFDYEPPPAEPE"
     gene            2428..3738
                     /locus_tag="VFV87_12360"
     CDS             2428..3738
                     /locus_tag="VFV87_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104601.1"
                     /translation="MKRSADRAVPPLSRRHFLAGGLATLAATASATADQPEGRQPATA
                     KAAGASRPLRVAAINSIFRLRSHSYHILGRMVYGFQKDGLHHQPNLQVVRMFNDQYPP
                     DDLSRSFCQRKGIELCKTAAETLGARGGLDVDAVALIVEHGDYPLNEYGQVLYPRYEY
                     FQEVVRVFEKAGKSVPVFVDKHLSYDHRHAAEMVATGKKLGFGMMAGSSLPVTWRVPQ
                     IEPPRETEFVEAVATFGFDRGAPEIYFIHALEVLQCMLERRKGGETGIKSVVCLEGDA
                     VWKAADEDRFSWPLVEEAVGRCQSANVGPIRENVTKPMAILLEYRDGTRGAVVNLIEA
                     TSEFAFAGRIRGRSEPLSCCFYLPAPPGARFFDPLTWHIEQFFHTGQPPYPVERTLLT
                     STILDLALHSRKEDGRPQASEALDIRYVPPVGSGFFRGPIADRE"
     gene            3917..5725
                     /locus_tag="VFV87_12365"
     CDS             3917..5725
                     /locus_tag="VFV87_12365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5104602.1"
                     /translation="MDFSIIVPLESHRGMAEQCLDGWVTQAFPRERFEILLAAPEKHD
                     QAHLHALANRLAAHDRIIPLPLDHDLALVAEAARQAQGEILVFTEAHCVPERNFLEQS
                     AAIFADHPDWSGFSGRSIPVTHNLLSEIEAEMYNRDITRNMERHQWLKVLDQCFVIRQ
                     DAYRACGGVEPQFGHFAEWLLAARLHRQGSRIGFDPRAAIHHYYVGDLEALEEFTLDF
                     AAGQMKFAQSAATDPCGDLFDEVPAWTARHHLNPQVVSCFLRLLANDLFQRAREHGAA
                     PHRLLTTLASWPWRTAWKWLARRNASRRASIGHMRRSISRLLWALRRDLRRGNSSRAS
                     LEFERLVNGWVRLARQQYLLDQEGEHDAYAPQPRENYSEWVPGVFETQPACGFHNFEV
                     RGRERFRWSEPSAMLWLPPLKGDWKIGIEWAQLRPFSDWGAARFYLNEQQILPANISH
                     RDFRTEIKYSGDGSTSLRLGWLCPALVTKEDDRHLGLPVSRVAWSDDDQRHKMPVTAA
                     RSHESFYFLHVPKCAGTTTRQILANAFAARDILSAYDVSLYYARQLKSCPEIYQPYTL
                     AYGHFRWELPTLVSDRRWRILTIVREPLERLLSAYD"
     gene            5801..6736
                     /locus_tag="VFV87_12370"
     CDS             5801..6736
                     /locus_tag="VFV87_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104603.1"
                     /translation="MILGHFIPLLSDTEAQGAPSVGALLAEHLTTALTNLNACELVGL
                     QEQLDDSVNLFCAATGMLPPRHVARVNITLHRTRQAEIDGRARELYETYLRPERELYA
                     AAEGIFRRQLADLHRRLAAEVGRELDTSGVRDVLRRRYFERRSREIVEAGIPSEIAWS
                     PADNFPGRNLHDRERHGGQSLRWTGPAPDTVFYFPLQPRRRVKMAIRLHPATPHTHAD
                     RARLQVNGAEISLLVERSADGYLLTGVIDPNQPPEECGVLSEFTLTTPVVRGAGEFRE
                     LGVALEQISIEPHRMVVPAPHRLGERSAAPLTASR"
BASE COUNT         1251 a         2201 c         2196 g         1349 t
ORIGIN      
        1 caggcgtatt cgtagagagg catggaattg cgaattgcga ttgtggattg cggattttgc
       61 tattgttata ggagtccgtc gtcagggaaa ggtcactgtc atgcgagcct tgagtcgtgc
      121 tgaagtgcgc gaagtcgatc ggcgggcgat cgaacagcat ggcatgagcg gcctggtgct
      181 catggagaac gccgggcgcg ggtgcgtcga tacgctgatg tcgcttggct gcaaggggcc
      241 ggtggcggtt gtctgcggca aggggaataa cgcgggggac ggcttcgtaa tcgcccggca
      301 cttggagatt cggggaatcc ccgtcacagt aattctgcta tgtgccccgg ccgagttgcg
      361 cggcgacgcg gcagccaact atgctatcct ggccaaaggc gacagaccgc tcgtggactt
      421 gtctgggcga tactcctccg attcatttca agcagaactc gttggcgcgg actggatcat
      481 tgatgctctg ctcggaacag gcgccaccgg cgctccgcgc ccgccgatgg acgatgcgat
      541 ccgactgatg aacgccagcc ccgcttgccg actggccgtc gatctgccca gtggcctaga
      601 ttgcgacacc ggatcgcctg ctgagccgac gatccgggcc gatcacacct gcaccttcgt
      661 cgcacccaag attggcttcg caagtcctaa ggcggtcccg tatctgggcg aagttcacgt
      721 cctcgatatc ggcgtgccgt ccggagtggt ggatcaagtg agtggtgagt cgttcctggc
      781 ttccgacctc tgacctcccc tcactccagc ttccgcgtcc aaaaccacgt ctggggctcc
      841 gtgggctcaa agtccgcccg ccgatatgcg cccatcgcgc ggaggttgtc ggagcggact
      901 tcgagcgtca ccttgcagca gccaagttcg cgggcgtgcc gttcaaccgc ggcgagaagt
      961 gcccggccga cgccctgacc gcggctgcca ggacggacgg cgatatcgtg gatattcacg
     1021 agcggcttgc cacgaaatgt cgagtagccg acgaagcaaa ttgccaggcc gacgggcttc
     1081 tcgtcctcat acgccagaaa aaccagcgcc ccaccgtgcc gcgccatccc cggaatcagg
     1141 ttcttgcggg cctcctccga aagcggcctc ccgtcgccga acgggtcgcg gcaatacatg
     1201 tcgagcagct cgacgatcgc cgcttgatgg gcgagatcgg ccaagttagc gagaagaacg
     1261 gtcatgtgac cttccgcgct ccgtatttgc gcgcccgctt cattccggct ctgccggcgg
     1321 cggctcgtaa tcgaaatcct tccacttcat ggcctggtgg aaaggctcga tgcggagcat
     1381 cacttcacca tcctggaaga tgcggacggt gccgctcgat tggctgacga ccaccgcgat
     1441 cgcgttggtc accttgctga tggcggcccc ggcccagtgg cgggcgccga gccccttgga
     1501 aagcgtgagg ttggcgtacg gggcatcgac gagctgagcc gctttttcga cgatcgcttc
     1561 gccattgaca atgaacccgc cgtccagggc ggccacctcc ttgatcgcct cccgcacgcg
     1621 cggatcgtgc aggtcgcgtt cggctcgcga ataacctttg acgggatcga agcccgccgg
     1681 ctggcagagc tgcaagacct tgcgcgtgtc gcccacaacg aacatggtgc cgacggcctt
     1741 gccttcacgt ccctcgcggc caatctcgac cgccaggtcg atcaccgttt tgagggtctc
     1801 caagggaacg ctcgtttcaa gctgccgcag gtcgcgggcg gtcagccggc ccaagtgctc
     1861 ttcaagctgg atgtagctga tcgagtcgat ggtatcgacc tcgaagccgc tataaacgac
     1921 gacgacactg gctccgggag ccaggatttc gcgggcgacg cccgtcagca gagcctgcgt
     1981 gagcttctcg atgaccggcg catcgggcat gttcaggaca atcgtggcca gcccggcttc
     2041 gggggctccc tccaggtgct cgcccgattc ggcggcgacg acgactttca gcccgtccag
     2101 ccgcttcagc agttcggccc actcgatcct ggcttccaac ataaccaaaa gcgcatcggc
     2161 ctccgtctcg cggctgaggc gcgtggcaag gtccagcaag gcagcaaact gaggatcgaa
     2221 ggccgggttt tccatgccga cgggcgtgat tacagggcga tattggccga ggaggagcgg
     2281 cgaaacggaa gctgcggcgg catcgtacgc ggtgatatcc atgtcggcaa ccgcctggag
     2341 cggaattcgc tccgtttgtt gcttcggcaa ggcggcgcgg ctaggtttgg tcgaaatcat
     2401 cgcgccgcat gccgtgaggg cctatctatg aaacgctccg cagatcgcgc tgttccgcca
     2461 ctttcccgcc gccacttcct ggccggtggg ctggcaacgc ttgccgcaac cgcgtcggct
     2521 accgccgacc agcccgaagg caggcaaccc gcgacggcga aggcggctgg agcgtcccgt
     2581 ccgctccgcg tggcggccat caactccatt ttccgcctcc gttcgcactc gtatcacatt
     2641 ctgggacgga tggtgtacgg ctttcagaag gacggcctgc accaccagcc caacctgcag
     2701 gttgtgcgga tgttcaacga ccagtacccg ccggacgact tgagccggag cttctgccag
     2761 cggaagggga ttgagctgtg caagaccgcg gccgaaacgc taggcgcacg aggcgggctg
     2821 gacgttgatg ccgtcgccct gatcgtcgag cacggcgact atcccctgaa tgaatatggc
     2881 caggttctct atccacgtta cgagtatttc caggaggtgg ttcgggtctt cgagaaagcc
     2941 ggcaagagtg tcccggtctt cgtcgataag cacctttctt acgatcaccg ccacgcagcc
     3001 gaaatggtcg ccacgggcaa gaagcttggg tttggcatga tggcggggtc ctcgctgccg
     3061 gtcacgtggc gggtgccaca gattgagccg ccacgggaaa cggagtttgt ggaggcggtc
     3121 gccacgttcg gatttgaccg cggagcgccg gagatctact tcatccacgc cctggaggtg
     3181 ctgcagtgca tgctggagcg gcgaaagggg ggcgaaacgg ggatcaaatc ggtcgtctgc
     3241 ctggaggggg acgccgtctg gaaggccgcg gacgaggacc gctttagttg gccgctggtg
     3301 gaagaggcgg tcgggcgatg tcagagcgca aacgtgggtc ccatccgcga aaatgtcacc
     3361 aagccgatgg caattctctt ggagtatcgg gacgggaccc gcggggcggt cgtgaatttg
     3421 atcgaagcca cgtcggagtt tgccttcgca gggcgaattc gcgggcgcag cgagccgctg
     3481 agctgctgct tctatctgcc ggccccgccc ggggcccggt tttttgaccc gctgacctgg
     3541 cacatcgaac aattcttcca caccggccag ccgccgtatc cagtcgagcg gacgcttctc
     3601 accagcacga ttctcgatct ggcattgcac tcccggaagg aggacgggcg gccccaagcg
     3661 agcgaggcgc tcgacatccg ctacgtgccg ccggtgggca gcggcttctt tcgcgggcca
     3721 attgccgatc gggaataagt ttccagctag cagggccttt ggcgcatggt tggcgtcatt
     3781 gtaagaactg cccttccttg gtttggggat tgcggcgatt attgcccgca tcttctcgaa
     3841 cctgaggaag ctagcagtcg aggcgggatc tccaggctgc tgtggacctc tctaccttcc
     3901 acggtggtgc aaggaaatgg atttctcgat catcgtcccg ctcgagtctc accgaggaat
     3961 ggcggagcaa tgtctggatg gttgggtgac gcaagccttc ccgcgcgagc ggttcgagat
     4021 tctcctggcg gccccggaaa agcacgacca ggcgcacttg cacgcccttg ccaaccggct
     4081 ggctgcacac gaccggatca ttcccctgcc gctggatcac gatctggcgc tcgtggccga
     4141 ggccgcccgc caggcccaag gcgaaatcct cgtgtttacg gaagcgcatt gcgtgcccga
     4201 gcgcaacttt ctcgagcaga gcgctgccat tttcgccgac catccggatt ggtcgggctt
     4261 cagcggccgc tcgatcccgg tgacgcacaa cttgctgtcc gagatagaag ccgagatgta
     4321 caaccgcgac atcacgcgca acatggagcg gcaccagtgg ctgaaggtgc tcgatcagtg
     4381 cttcgtgatc cgccaggacg cctaccgggc gtgcggcgga gtggagccgc agtttggcca
     4441 tttcgcggaa tggcttttgg cagcgcggct gcaccgccag ggctctcgca tcggtttcga
     4501 tccccgggcg gcaatccatc actattacgt gggcgacctc gaggcactcg aagaattcac
     4561 gctcgacttt gcagcgggcc agatgaagtt cgcgcagtca gcggcgaccg atccgtgcgg
     4621 cgacttgttc gatgaagttc ccgcttggac cgcacgccat catttgaatc cccaggtcgt
     4681 gagttgcttc ttgcgcctgc tggcgaacga tctattccaa cgagcgcgcg agcacggggc
     4741 tgcaccacac cgtctgctga cgacactcgc ctcctggcct tggcgaactg cttggaaatg
     4801 gctcgcgaga cggaatgctt cccgtcgcgc gtccatcggg cacatgcggc gcagtatttc
     4861 tcgactcttg tgggcgctga ggcgggattt gcggcgagga aactcgtcgc gcgcctcgct
     4921 ggaattcgag cgtctggtca acggttgggt tcgcctcgcc cggcagcagt acttgctgga
     4981 ccaggaaggc gagcacgatg cctacgcgcc gcaacctcgc gagaactatt ccgagtgggt
     5041 tccaggcgtt tttgagacac aacctgcctg tggcttccac aacttcgaag ttagaggcag
     5101 agagcgcttt cgatggtcag aaccgtcggc catgctctgg cttccgccgc tcaagggcga
     5161 ttggaagatc ggcattgagt gggcccaact tcgtccattt tccgattggg gcgcggcgcg
     5221 cttctacctg aacgaacagc agattctgcc cgcgaacatt tctcaccgcg atttcaggac
     5281 ggaaatcaaa tactcaggtg acggctccac gagccttcgc ctgggatggc tctgccccgc
     5341 actcgtcacc aaggaggacg accggcatct tggcctgcct gtctcacgtg tggcctggtc
     5401 ggacgatgac caacgtcaca agatgccagt cacggcagca cgatcgcacg agagttttta
     5461 tttcctgcac gtgcccaagt gcgccggcac aacgacgcgg caaattctgg caaacgcatt
     5521 tgcggcgcgc gacattctct cagcctacga cgtctcgctc tattatgcgc ggcagttgaa
     5581 gagttgtcct gagatctacc aaccctatac cctggcgtac ggccactttc gctgggaact
     5641 cccgacgctt gtctccgacc gccgctggcg aattctgaca atcgtacgcg aacctctcga
     5701 acggctgctt tcggcttatg actaatcgaa gcagcagcga cgccttacgc cgggcctcga
     5761 ctttgccgcc tggatcgagc aagagctgca gctatcgcac gtgattctgg gccacttcat
     5821 tccattgctg agcgacacgg aagcccaggg cgctccatcc gttggagcgc tgttggcgga
     5881 gcatttaacc acagctctga ctaacctgaa cgcgtgcgag ttggttggtc tgcaagagca
     5941 gcttgacgac agtgtcaatt tgttttgcgc cgccacggga atgctgccgc cccgccacgt
     6001 tgcccgcgtc aatattaccc tccaccgcac gcgccaggcg gaaatcgacg gacgcgctcg
     6061 cgaactgtac gagacctacc tgcgacccga gcgggaactg tatgcagcag cagaaggcat
     6121 ttttcgccgg cagctggccg acctccaccg gcggctggcc gcagaagtgg ggcgcgaact
     6181 cgacacatca ggcgttcgcg atgtgctgcg acgccgctac ttcgagcggc ggtcgcgcga
     6241 aatcgtcgaa gctggcattc ccagcgagat cgcctggtcg cccgccgaca actttccagg
     6301 ccgtaacctg cacgaccgtg agcgccacgg cggccaatcc ctgcgctgga cgggccctgc
     6361 cccggatacc gttttctatt tcccgctcca gccacgccgc cgggtcaaga tggccatccg
     6421 cttgcatccc gccactccgc atacgcatgc ggaccgcgct cgcctgcagg tcaacggagc
     6481 agaaatttcg ctgctcgtcg aacggagcgc agacggctac ctgctgacgg gcgtcatcga
     6541 tccgaaccag ccgccggaag aatgcggtgt cctctcggag ttcacgctga ccacgcctgt
     6601 cgtccgcgga gccggcgagt ttcgcgaact gggcgtcgcg ctggagcaaa tcagcatcga
     6661 gccgcaccgg atggtcgtgc ccgccccgca tcgattgggg gaacgctcgg ccgctccgct
     6721 gaccgccagc cgctgagcct tcgcgggcct ggtcgtagta tcatggcccg ctatggcgaa
     6781 ttgtccgcat tgcgctgaac cgttcggctg ggcggtgatc ttgcgccact ctggcgattg
     6841 cggccattgc gggctgccgc tgcgggtggg cgcgggcggc aagctggaaa cctacgccag
     6901 ccggcatgcg gcggagattc tcagtcagac gccgacctac cgctattggc tcctgctggg
     6961 gatcggcgtg gcggcgacct tcttgatcaa cctggtt
//
LOCUS       DASUGB010000647         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1460995, whole genome shotgun sequence.
ACCESSION   DASUGB010000647 DASUGB010000000
VERSION     DASUGB010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460995"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFV87_12375"
     CDS             <1..646
                     /locus_tag="VFV87_12375"
                     /inference="COORDINATES: protein motif:HMM:NF026121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HEX5104604.1"
                     /translation="DKVRALATRSGGERIEVTPEDFARLLEGLRAETFDPGKRSFIES
                     LSPTDLFTSEQVRQLLSEFDFDIDREKAAVALHPRVIDPENFYRALQVFESDWSAVTR
                     KLSLDRAAKSLKLEPGEAVSDQELNALVAAMKTAPLDQGKCAFLKVIVHGRAFTCDQA
                     AKLLSQFDFDTDREQAALAIYPRLSDPQNLYRVLAVFTFDSGRESVRNKIETVK"
     gene            complement(756..1085)
                     /locus_tag="VFV87_12380"
     CDS             complement(756..1085)
                     /locus_tag="VFV87_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5104605.1"
                     /translation="MSEYYVSRQETSHHEIFPGVHIFTCAGERMMLSLVEFEPRAIVQ
                     PHSHPHEQMGMLLEGELTFTIGGETRTLHPGEMWRIPGGVIHSAVAGEQPVKALDVFH
                     PVREDYR"
     gene            complement(1122..2480)
                     /locus_tag="VFV87_12385"
     CDS             complement(1122..2480)
                     /locus_tag="VFV87_12385"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5104606.1"
                     /translation="MKITRVHAREILDSRGKPTVEAEVHLEGGIVGRASVPSGASTGT
                     AEACELRDTSDPWYDGFGVRQAVRNIREIIAPALEGEEFPGIASINRIDRLPNHDDVY
                     VEHSGPPGIDARLLKIDGTANKSRLGANAILAVSLASMRAFTRLVEIPLYKSISYEMH
                     TVRLAGYFEGLYAEPQMPLPMVNMISGGKHAGGNLDFQDVLIIPVGARDYPTQLEWIV
                     RIYWRLGKLLTEAGYEGRLVGDEGGHGPRLNSNREAVEFVMRAIEAAKLRPGEDVTIA
                     LDVAATHFYRDRGYLLAAERGQKLTSSQMIDRLESWVNDFPITSIEDGLAENDWEGWK
                     ELTSRLGGRVKLVGDDLFATNERLMQRGIDEKVANAVLIKANQIGTLTETFRTMLLAR
                     QHGYACIVSARSGETEDDFLADLAVGTAAEYIKIGSVARSERLAKYNRLQRIAEELSQ
                     AE"
     gene            complement(2517..>3673)
                     /locus_tag="VFV87_12390"
     CDS             complement(2517..>3673)
                     /locus_tag="VFV87_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104607.1"
                     /translation="RIPTALALANRLKAMEHALSLDTRVLKPGESLGGSAKLPPDDSA
                     NARLGKTTASRTPGITTPLTPDEQGEYRLAGESPTIMTSGGSVLSSGSTQAKPKATRV
                     TGIGSAADPGTEATLAPAAPGAEYEASPVKATRFTTVSEIELRAAGLRQGGEETDWRQ
                     WLALGGIVAVVLPLIGVGIYFATRSPTADQLYARIQSAAEGGPEGLTAAGDDVARFLE
                     AFPSDARAEEIRGYQEDLELDSLERRFEFRARRAKGVESLSPVERAYLEAVQLRSTDP
                     EAALARFQALVAVFEGPADPTQNNLQQRTSEQCLQLAKKQIERLEPTVKKINQEQRLA
                     VRRQLSRAAKLAETDRGAAEEIWQGIITLYAGKDWAKDLVEQAQTQFAGP"
BASE COUNT          613 a         1219 c         1105 g          736 t
ORIGIN      
        1 ggataaggtc cgcgccctgg cgacgcgcag cggcggcgag cggattgagg ttacgccaga
       61 agatttcgcg cggctgctgg agggcttgcg cgcggagacg tttgatcctg gcaagcgctc
      121 gttcattgaa tcgttgagcc cgaccgatct tttcacgtcg gagcaagtcc gccaattgct
      181 gagcgagttc gacttcgaca tcgaccgaga aaaggccgcc gtagcgcttc atccgcgagt
      241 gatcgatccc gagaacttct accgggcgct gcaggtcttt gagtcggatt ggagcgccgt
      301 cacccggaag ctgtcgctcg atcgtgcggc aaagtccttg aaactagagc ccggcgaggc
      361 ggtttcggat caggagctca acgcgctcgt cgccgcaatg aaaaccgctc cgctcgatca
      421 aggcaagtgc gcctttctga aggtgatcgt ccacggccgc gccttcactt gcgatcaggc
      481 agccaaactg ctatcgcagt tcgacttcga tacggaccgg gaacaggccg cgctggccat
      541 ttatccgcgg ctgagcgacc cgcagaatct ctatcgcgtt ctggcggtct tcacgttcga
      601 ctccggccgg gaatccgtga ggaataaaat tgagacggta aagtgattgg ccggttcgtc
      661 ggtgcgccgg cgatctctcc aggccaagcg aatgcgcaat ccggaatagg ttcacctgct
      721 gccctcattc cgcattccgc tctccgactt ccgcattagc ggtagtcctc ccgcaccgga
      781 tgaaacacat ccagcgcctt gaccggctgc tcgcccgcga cggcgctgtg gatcacgccg
      841 ccgggaatcc gccacatctc gccgggatgc agcgttctgg tttcgccgcc gatcgtgaag
      901 gtcagttcgc cttccagcag catccccatc tgttcgtgcg gatggctgtg cggctgcacg
      961 atggcgcggg gctcgaattc gaccagcgaa agcatcatcc gctcaccggc gcaggtgaag
     1021 atgtgcacgc cggggaagat ctcgtggtgc gacgtttcct gccggctaac ataatactcg
     1081 ctcatggcac tcaaagccgc gaccagcggt cgcggttccc atcattccgc ctgtgagagt
     1141 tcctctgcga tcctttgcag ccgattgtac ttggccagtc gctcgctgcg cgcgacggag
     1201 ccgatcttga tgtactcggc ggcggtgccg acggccagat cggccaggaa atcatcctct
     1261 gtctcgccgc tgcgggcgga aacgatgcag gcataaccgt gctgccgggc gagcaacatc
     1321 gtgcgaaagg tttcggtcag cgtgccgatt tgattggcct tgatcaggac cgcgttggcc
     1381 actttctcat caattcccct ctgcatgagc ctttcgttgg tcgcaaacaa atcgtcgccg
     1441 acaagcttca cgcggccgcc gagtcgcgag gtcaactcct tccagccctc ccaatcgttc
     1501 tctgccaatc cgtcttcgat gctggtgatg ggaaagtcat tgacccagga ttccagccgg
     1561 tcgatcatct ggctgctggt cagcttctgg ccccgttcgg ctgccagaag atagccgcga
     1621 tcccggtaga aatgcgttgc ggcgacatcg agggcaatcg tcacgtcctc gcccggccgc
     1681 agtttcgccg cttcaatcgc ccgcatcacg aattccaccg cttcgcggtt cgaattcagc
     1741 cgcgggccat ggccgccttc atcgcccacc agccgtcctt cgtagccagc ctccgtcagc
     1801 agcttgccca atcgccaata gatgcgaacg atccactcca attgcgtcgg atagtctcgc
     1861 gcgccgacgg gaatgattag cacgtcctga aaatcgagat tgcccccggc gtgcttgccg
     1921 ccggagatca tgttgaccat tggcagcggc atctgcggct cggcgtacaa tccttcaaag
     1981 tagcccgcca atctgacggt gtgcatttca taactgatcg acttatacag cggaatctcg
     2041 accaaccggg taaacgcgcg catcgacgcc aacgaaacgg ccaagatcgc gttggccccc
     2101 aatcgcgatt tgttggctgt gccgtcgatc ttcagcaacc gcgcgtcaat ccccggcggg
     2161 ccgctgtgct ccacgtacac gtcgtcgtga ttcggcagcc ggtcgatgcg gttgatcgag
     2221 gcgatgccgg ggaattcctc gccctccagt gcaggcgcaa tgatctcacg gatattcctc
     2281 acggcctgcc gcacgccaaa cccgtcgtac cacgggtcgc tcgtatcccg cagctcgcac
     2341 gcttctgccg tgccagtgct cgcgccgctg gggacgctgg cccggccgac gatgccgcct
     2401 tccaggtgca cctcggcctc gaccgtcggc ttgccgcggc tgtcgaggat ttcgcgggcg
     2461 tgaacgcggg tgatcttcat gcttgtcgta ggacggacct ctggtccgtc cggcattcac
     2521 ggaccggcaa actgagtttg ggcctgctcg accaggtctt tcgcccagtc cttgcccgca
     2581 tagagtgtaa taatcccctg ccagatttcc tccgccgccc cgcggtctgt ttccgccagc
     2641 ttcgccgccc ggctaagctg ccgccgcacg gccaagcgct gctcctgatt gatcttcttg
     2701 accgtcggct ccaaccgctc gatctgcttc ttggcaagtt gcagacattg ttcgctggtc
     2761 cgctgctgca ggttgttttg ggtcggatcg gcggggcctt caaacaccgc gacgagggcc
     2821 tgaaagcggg ccagagccgc ttctggatcg gtggaccgga gctgcacggc ttcgaggtag
     2881 gctcgctcga ccggcgacag cgactcgacc cccttggccc gcctggcccg aaattcgaac
     2941 cgccgctcca ggctgtcgag ctccagatcc tcttgatagc cgcggatttc ctcggcccgt
     3001 gcatcgctgg gaaacgcttc gagaaagcgg gccacgtcgt cacccgcagc agtcagcccc
     3061 tccgggccgc cttccgcagc cgactggatg cgggcgtaga gctgatcggc ggtcggtgag
     3121 cgggtggcga aatagatgcc gacgccaatg agcgggagca ctacggccac gattccaccc
     3181 agcgcgagcc attgccgcca gtccgtttcc tcccccccct ggcgcaagcc agcggcccgc
     3241 aactcgattt cgctaacggt cgtgaatcgc gttgctttga ccggcgacgc ctcgtactcg
     3301 gcgcccggcg cggcgggagc caaggtcgct tccgttcctg ggtcggcggc agagccgatt
     3361 cctgtcactc gcgtggcctt gggcttcgcc tgcgtgctgc cggaggagag caccgagccg
     3421 ccgctggtca tgatcgtcgg cgactcgcct gccaggcgat attcgccctg ttcgtccggc
     3481 gtcagcggcg tcgtgattcc cggcgtgcgg ctggcggtgg tcttgcccag gcgggcattg
     3541 gcggaatcgt cgggcggcag cttcgccgag ccccccagcg attcgcccgg cttcagcacg
     3601 cgcgtatcga gtgagagcgc gtgctccatc gccttcagcc ggttcgccag cgccaaagcc
     3661 gtcgggatgc gct
//
LOCUS       DASUGB010000648         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_72419,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000648 DASUGB010000000
VERSION     DASUGB010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72419"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="VFV87_12395"
     CDS             <1..2021
                     /locus_tag="VFV87_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5104608.1"
                     /translation="NADKPYDRMVQEMLAADELVPGDIDAARATGYLVRSWYKFNRNK
                     WLDDAIEHTGKAFLGVTFNCCRCHDHMYDPLAQQEYYQLRAVFEPYDVRTDRVPGEAN
                     SEKSGLVRVFDAKPDAPTFLFVRGNEKDPVKDQPLLPQVPKVFSGVPFAVEQISLPPQ
                     AAYPGLLPFVREETLAADRAELEKAKTALSAAEAKWNEVNQAAVAASLPASEEGAPPQ
                     PPAPPQNVVDADSALAVARAAVAAAEAKMAQDEARLAADDANYATPKRGDAAELSKAA
                     ALAERVANLRQAQASLLQAEIALTVAQAAKKPEDMKTVKAVTEAEAALAAALKARSDA
                     LAALDQPGEAYSPLTPVYPATSTGRRLALARWITSRENPLAARVAINHIWLRHFGSPL
                     VPSVFDFGMNGKPPTNQPLLDWLAVELMDHGWQMKHIHRLIVTSRTYRLSSTAGMTND
                     QAPMTKEIQNPKSKIQNASIDPENLYYWRFNPRRLEAEAVRDATLFVAGSLDLTRGGP
                     ELDQNAGLTTPRRSLYFRSSKEKKMQFLSLFDSANPVECYRRSESIAPQQALAMANST
                     LTLAQSRLLAKKLSDSLGNVAAADSPRRFVEDAFVRILSRQPSEAELATCLEFLTEQA
                     AKLADAKSLTAFSAGPAASVAPSSDPAQRARENLVHVLLNHNDFVTVR"
BASE COUNT          432 a          722 c          689 g          345 t
ORIGIN      
        1 tcaacgccga caagccgtac gaccggatgg tgcaggaaat gctggcggcc gacgaattgg
       61 tgcccggcga catcgacgcg gcgcgggcca ccgggtacct ggtgcgaagc tggtacaagt
      121 tcaatcgcaa caagtggctg gacgatgcca tcgagcacac gggcaaggcg tttttgggcg
      181 tgacgttcaa ttgctgccgc tgccacgacc acatgtacga cccgctggcg cagcaggagt
      241 attaccagct gcgggccgtg ttcgagccgt acgacgtgcg aaccgaccgc gtgccgggcg
      301 aggcgaacag cgagaagtcc ggcctggtgc gggtgttcga cgccaagccc gatgcaccga
      361 cgttcctgtt cgtccgcggc aacgagaaag acccggtgaa ggaccagccg ctcttgccgc
      421 aggtgcccaa ggtgttttcc ggcgtgccgt ttgccgtcga acagatcagc ctgccgccgc
      481 aggctgcgta tccgggcctg ctgccgtttg tgcgcgaaga gacgttggcc gcggatcggg
      541 ccgagctcga aaaagcaaag acggcgctga gcgccgcgga ggcgaaatgg aacgaggtca
      601 atcaagcggc cgttgccgcg tcgctgccgg caagcgagga aggtgccccg ccgcagcccc
      661 ccgcgccgcc gcaaaacgtg gtggacgccg acagcgctct ggcggttgcc cgggccgcgg
      721 tggctgcggc ggaggcgaaa atggcgcagg acgaggcccg cctcgccgcc gacgatgcca
      781 attatgccac ccctaagcgc ggcgacgccg ccgagctatc caaggcggcg gccttggccg
      841 agcgcgtggc aaacctgcgg caagcccagg cgagcttgct ccaggcggaa atcgccctga
      901 ctgtagccca agccgccaaa aagccggagg atatgaaaac ggtcaaggcc gtgaccgaag
      961 ccgaagcagc gttggcggct gctctcaagg cgcggagcga cgccttggcc gccctcgatc
     1021 aaccgggtga agcctactcg cccctcacgc ccgtctatcc cgcaaccagc acgggccgcc
     1081 gcctcgcctt ggcccgctgg atcacctccc gcgaaaaccc gctggccgcc cgcgtggcga
     1141 tcaaccacat ctggctgcgg catttcggtt cgccgctcgt gccgtcggtc ttcgatttcg
     1201 gcatgaacgg caagccgccg accaaccagc cactgctcga ttggctcgcg gtcgagctga
     1261 tggaccacgg ctggcagatg aagcacattc accggctgat tgtgacgagc aggacgtatc
     1321 ggttgtcctc aaccgctgga atgaccaatg accaagcacc aatgaccaaa gaaatccaaa
     1381 atccaaaatc caaaatccaa aatgcgtcca tcgatcccga aaacctctat tactggcgat
     1441 tcaacccgcg ccggttggag gcggaagcgg ttcgcgatgc gacgctgttt gtcgccggct
     1501 cgctcgacct gacgcggggc ggcccggagc tcgatcagaa cgccggtctg actacgccgc
     1561 gccgcagcct ctatttccgg tcgagcaaag aaaagaagat gcagtttctc tcgctgttcg
     1621 acagcgccaa cccggtcgag tgttaccgcc gcagcgagag catcgcccca cagcaggccc
     1681 tggccatggc caacagcacg ctgacgctgg cccagtcgcg gcttctggcc aagaagctca
     1741 gcgattcgct gggcaacgtc gccgctgccg actcgccgcg gcgtttcgtc gaagacgcgt
     1801 tcgtccgcat cctctcccgc cagccgagcg aagccgagct ggccacgtgc ctcgaattcc
     1861 tgaccgagca ggcggcgaag ctggctgacg cgaaatcgct gacggcgttt tcggccggcc
     1921 ctgcggcgtc ggtcgcgcct tcgagcgatc cggcccagcg agcccgcgaa aacctggtcc
     1981 acgtgctgct caaccacaac gacttcgtga ccgtgcggta aacagcggag tttgcatcgg
     2041 tcaattaatg ccaaggcgca aagacgcgaa gacgcaaagg acggaaagga atagcgagga
     2101 gtttgaccat gtccatttcc cgtttccttc gcgcctctgc gtctctgcgc ctttgcgtta
     2161 acgaaagccg gcggcaattc ttgtcgga
//
LOCUS       DASUGB010000649         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1046059, whole genome shotgun sequence.
ACCESSION   DASUGB010000649 DASUGB010000000
VERSION     DASUGB010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046059"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFV87_12400"
     CDS             complement(<1..968)
                     /locus_tag="VFV87_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5104609.1"
                     /translation="MLLADSFLRFLPPLSPGQAFALAAAAIAATLLAGWLLGPANPVA
                     RRWSLWSLRAGIVGLVVLVILNPVQVDELPGPLKRPEVFYLLDASASMQMGNPRSRWD
                     QALEMIRAADRQAADSPAVVKPCRFGQRLAAIDQPDQVGVGAARAPALKITTASASSS
                     EPAGRNKRPIAPLDGDTRLQAALRQISSRFSRVPPQGIVVFSDGRVHDDTGLEQIAAG
                     FAKLKVPIHVVPVGDTEKGGDVAIAAVVAPPKVRKFTEVEVQVFVRSFGFEGKRSEVQ
                     LWELEAGREGRKLASMPITLQSGYQSVSLLFRTDLATRKLRVAIPPL"
     gene            complement(982..1887)
                     /locus_tag="VFV87_12405"
     CDS             complement(982..1887)
                     /locus_tag="VFV87_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104610.1"
                     /translation="MNVLVLAACLLAQTPAPLDRAREVEAREGEPPAQPGSAERGSAL
                     DYLHRQYRDDAAKYLFHADAERKQPLMLVEKPVLKWANDDDWSGDVFVWTHAGRPAVI
                     GCMLSGPSGEANRNAYHEFHLLAEQPIAPAGLLTRRNWEPEGGLARTPLPDAPPPANS
                     AAGRLVQMRQISRDFTAHMQADGMWELRLLPQPLFRFESDKIFAAAADKTDAIDGALF
                     AWVWTKGTDPEVLLLVECRRTEGGPAWQYAPVRFSNRTVWLTRGDKEVWRVESHREPA
                     GQSTSLIYTTAYARTFPREAVTKEP"
     gene            complement(1895..>2493)
                     /locus_tag="VFV87_12410"
     CDS             complement(1895..>2493)
                     /locus_tag="VFV87_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104611.1"
                     /translation="NLPGQVGAGKLQDAKANVGDGAERLEAAAEQLAALHRSIVAPQV
                     DELAKLEAQLTRLDEQLDQLDTPSRITAWHMDADKLLDELADAGISEELQKQFLEEMK
                     KGGWGPDLRNRGWQWARIEGGYYTAPGPYRVLLSRLLSSVRGRMQELMLGDLASSRDE
                     PIPPQYQELVDRYYQVLATEGKERLKAAPVAPADRSEK"
BASE COUNT          389 a          845 c          838 g          421 t
ORIGIN      
        1 agtggcggaa tcgccacgcg cagcttgcga gtggccagat cggtgcggaa tagcaggctg
       61 accgactgat agccggactg gagcgtgatc ggcatgctgg cgagcttccg cccctcgcgg
      121 cccgcttcga gctcccaaag ctgcacctcg ctccgctttc cctcgaagcc gaagctgcga
      181 acgaagacct gcacctcgac ctcggtgaat ttgcggacct tcggtggggc gacgacggcg
      241 gcgatggcca cgtcgccccc cttctccgtg tcgccgacag gcacgacgtg gatcggaacc
      301 ttcagcttcg caaaccccgc ggcaatctgc tccaggcccg tgtcgtcgtg cacgcggccg
      361 tcggagaaga cgacgattcc ttgcggcggc acgcggctga agcggctgga aatctgccgc
      421 agggcggctt gcaggcgggt gtcgccgtcg agcggggcaa tcggccgctt gttgcggcca
      481 gccggctcgc tgctgctggc cgatgccgta gtgattttca gggccggcgc acgggcagcg
      541 cccacgccga cctgatccgg ctggtcgatc gccgccaagc gctggccgaa ccgacacggc
      601 ttgacgacgg ccggcgaatc ggccgcctgg cggtcggcgg cgcggatcat ctcgagcgcc
      661 tggtcccacc gcgagcgggg attgcccatc tgcatgctgg ccgatgcgtc gagcaggtag
      721 aacacctccg gtcgcttgag cgggccgggt agctcatcga cctgcaccgg attgagaatc
      781 acgagcacca cgaggccgac aatccccgcc cgcaggctcc acaggctcca gcggcgggcc
      841 acaggattgg ccggacccag cagccacccg gccagcagcg tggccgcaat cgcggctgcc
      901 gccaaggcga acgcctggcc cggcgaaagc ggcggcaaaa accggaggaa tgaatcggcc
      961 aggagcatag ggaaaggtca gttaaggttc tttggtgacc gcttcgcgcg ggaaggtgcg
     1021 ggcgtaggcg gtggtgtata tcagcgacgt cgattggccc gccggctcgc ggtggctctc
     1081 gacgcgccag acttccttgt cgcctcgcgt cagccacacc gttcggttcg agaagcgcac
     1141 cggagcatac tgccacgcgg gaccgccctc tgtccggcga cattcgacga gcaggaggac
     1201 ctcggggtcc gtccccttgg tccagaccca ggcgaatagc gcgccgtcga tggcatcagt
     1261 cttgtctgcg gccgcggcaa aaatcttatc gctttcaaag cgaaacagcg gctgcggcag
     1321 caggcgcagc tcccacatgc cgtcggcctg catgtgggcc gtgaagtcgc gcgagatctg
     1381 ccgcatttgc accagccggc cggcggccga gttggccggg ggcggagcgt ccggcagcgg
     1441 cgtacgggcc aggccccctt ccggctccca gttgcggcgg gtcagcagcc cggccggggc
     1501 aatcggctgc tcggccagca ggtgaaattc atgataggcg ttccgattgg cttcgccgct
     1561 ggggccggag agcatgcagc cgatgaccgc gggccggcct gcgtgcgtcc agacgaacac
     1621 gtcgcccgac cagtcgtcgt cgttggccca cttgagcacc ggcttctcga cgagcatcag
     1681 cggctgcttt cgctcggcgt cggcatggaa caggtatttc gcggcgtcgt cgcggtactg
     1741 gcgatggaga tagtccaagg cgctgccgcg ttctgcggag cccggctggg caggaggctc
     1801 gccctcccga gcctcgacct cccgagccct gtccagcggc gccggggttt gggcgagcaa
     1861 acaagcggct aatacgagca cgttcatggc gggcctattt ctccgagcga tcggccggtg
     1921 cgaccggcgc cgctttcagc cgctctttcc cttcggtcgc cagcacctgg taatagcgat
     1981 cgaccagctc ctggtactgt ggcggaatcg gctcgtcgcg gcttgacgcc agatccccca
     2041 gcatgagctc ttgcatccta ccgcgcaccg aactgagcaa gcgagaaagc agcacgcggt
     2101 acggccccgg ggccgtgtag tagcctcctt cgatccgtgc ccactgccag ccgcgattgc
     2161 gcaggtccgg cccccaccca ccctttttca tttcttcgag gaattgcttc tgcaattctt
     2221 cggagatgcc ggcgtcggcg agctcgtcga gcagtttatc cgcgtccatg tgccaggcgg
     2281 tgatccgcga cggcgtgtcg agctggtcga gttgctcgtc cagccgcgtg agctgcgctt
     2341 cgagcttggc cagctcatcg acctgcgggg cgacgatcga gcggtgcagc gcggccaatt
     2401 gctcggccgc cgcttccagc cgctcggccc cgtcgccgac gttggctttg gcgtcctgga
     2461 gctttcccgc gccgacctgt cccggcagat tct
//
LOCUS       DASUGB010000650         5687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_351339, whole genome shotgun sequence.
ACCESSION   DASUGB010000650 DASUGB010000000
VERSION     DASUGB010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351339"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFV87_12415"
     CDS             <1..923
                     /locus_tag="VFV87_12415"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEX5104612.1"
                     /translation="DELRRQLGRMFADPKFERFLDSFPYQWLRLHQVGMFPPNPKLYP
                     DYSPWLERSMVQETKLYFREMFQQNRPLRELLDTDWTMLNPRLREHYGLPAADEASFQ
                     RVALGSSEQRGGLLTQASILMLTSDGQRHRPVHRGVWLSEAIFNRTPPAPPPNVEPLE
                     PTAEDAPKATIRAQLAAHATNATCASCHSRIDPLGFAFENYDAIGRWRTEEEGAGKGR
                     NPPVNAAGELPDGRKFDNAREFKALLIQDQDRIAEAFVEQLATYALRRVMTIDDAEQI
                     HAIAGASQNDEYRLRTVIENFVHSDLFQKR"
     gene            933..2168
                     /locus_tag="VFV87_12420"
     CDS             933..2168
                     /locus_tag="VFV87_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104613.1"
                     /translation="MPRSLRSIRFLLATAAGLVLAAGLTGPLAAQETGAAPAYGEFPA
                     ADGGKRWMGELIDVDPINRRGLIRPGGDLAPDRYNRFLGQPFAMLPYGMIYYHGAPAE
                     LKDIPLGTVLHGVLYQPPPGDDSVPRPDPQDKVDAQRAKYFVEHTHALLLEDDVSFYR
                     RRGQAWKVTGIETSEMDGRQFLVVTSVGKEAPGGLHGEQKFCIDKSTRVWQGKEFAGL
                     GEIQAGQAVHVNLTWDPGWGYGKFHVSDVWLDQQAQDVAAEVQRQTHLRYQRYHWPAG
                     WVDRVTYDPSRPNGAGSVYVTLSGGLDPSLDEEIIDAKRAEIAIAEPTLRTWRKDHDA
                     QGGQILEVKRVPDPPLGSSGIQLRVEVPKVPEGFRDGGIVRVGTTSFPAGILPPEERV
                     NGLEDRSRSLSGSVAPSQE"
     gene            2181..3566
                     /locus_tag="VFV87_12425"
     CDS             2181..3566
                     /locus_tag="VFV87_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5104614.1"
                     /translation="MANYLAQNWLLNRRHVLKGIGVSLALPFLECMRPLRAVEATPRV
                     KRSVFMYLPNGVNPADYQITSSGKDYQLSRSLQSLKRHRANITPISGLYHPHGMGKAH
                     DCSNIWLTGARHDANHRNTISVDQLMARVTEPHTRFPSLQLSSENRRCLAWTAGGVAL
                     PDDSVPSVVFRGLFEEPKSGVAVQRRELNRRGSILDSVLDEARRLDNQLGNQDSERLE
                     QYLTSVREIEIRTQRADGWLDKPRPKIDKASAAQLDRDIPLEELGEFLRTMYDLIVLA
                     FQTDSTRVVTFMTGDEGTGPAIPEIGINFGRHALSHHNGNKEMLANLSASDAFNIEQF
                     AYFLDRLAETRDAEGPLLDTTLALYGSGMAYGHSHGNANLPLVLAGGKSLGIRHGRHL
                     DYNDLASPAGHNYQADQPGALVRICHKPVNPKAHMSNLLLTMAQAMDVPAEQFGDSNS
                     EASELTYSPRF"
     gene            3581..3718
                     /locus_tag="VFV87_12430"
     CDS             3581..3718
                     /locus_tag="VFV87_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104615.1"
                     /translation="MPLPQIADKLGSNTNAIYKLLHDARKTLRRGLEASGFTVEDVRK
                     A"
     gene            3734..4252
                     /locus_tag="VFV87_12435"
     CDS             3734..4252
                     /locus_tag="VFV87_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104616.1"
                     /translation="MALNSGQVRRLLKMIRQTREAEMTCPECLDELDKYTQKTIDGTP
                     IDGVLVNVREHLEACPFCMDQFNLVLEALQAIDELYAAPVTSTSCSSRSLFVLEVGPT
                     SADYGRRKLSAVSFVKRHRCSWQDPRRASGRCNRYELAMGETAGGVLLLRTLTGASTL
                     GSCRLVGPRERA"
     gene            4289..4927
                     /locus_tag="VFV87_12440"
     CDS             4289..4927
                     /locus_tag="VFV87_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104617.1"
                     /translation="MTDVTQILSQIEQGDPNAAEQLLPLVYDELRKLAAARMAAEAPG
                     QTLQATALVHEAYLRLVAGGQGAEDLRLGAEEIRSAVLPPVPSPQPPVPLSAFNSRGH
                     FFAAAAEAMRRILVERARRRGRVRHGGEFQRQGAAEAELFEDQRQAEMLQVDAALDEL
                     AEEDPRAAELVKLRFFVGMTTAEAAEALGVSTKTVERSWVYAKAWLREALSR"
     gene            5030..>5687
                     /locus_tag="VFV87_12445"
     CDS             5030..>5687
                     /locus_tag="VFV87_12445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5104618.1"
                     /translation="MHERDILLATLEMPGTTERAEYLARVCGMDTELRGRVEALLRAS
                     EVQDSFLEHPAAETELFSRETDGIRREIAAHEELSLDFLQMADEPGALGRLGPYTITE
                     MIGRGGMGVVLKARDSKLNRIVAIKVLAPELASNPTARKRFQREAHAAAAVVHQHIVT
                     IHAVDEDRLPYLVMECIAGQSLKEKIDREGHLPLVEILRIGQQVASGLAAAHAHGLIH
                     R"
BASE COUNT         1132 a         1809 c         1778 g          968 t
ORIGIN      
        1 gcgacgagct gcgccggcag ctcgggcgga tgttcgccga cccaaagttt gaaaggtttc
       61 tggactcttt tccctatcag tggctgcggc tgcaccaggt gggcatgttc ccgccgaatc
      121 caaagctata tcccgactac agcccgtggc tcgagcggag catggtgcag gagacgaagt
      181 tgtattttcg cgagatgttc cagcaaaacc ggccgcttcg cgagttactg gacacggact
      241 ggacgatgct caacccccgc ctgcgagaac actacggcct gcccgccgcg gacgaggcga
      301 gctttcagcg ggtcgcgctc ggttccagcg aacagcgcgg tggactcctc acgcaggcct
      361 cgattctgat gctcacatcc gacgggcagc ggcatcggcc ggtgcatcgc ggcgtgtggc
      421 tctcggaagc catcttcaac cggacgccgc cggctccgcc gcccaacgtc gagcccctgg
      481 aaccgactgc cgaggacgct cccaaggcga cgatccgcgc gcagcttgcg gcccacgcga
      541 cgaacgcgac ctgcgcctcg tgccacagca gaattgatcc actgggcttc gcgtttgaga
      601 actacgacgc catcggccgc tggcgcacgg aagaggaagg ggcgggcaag ggtaggaatc
      661 cgccggtcaa cgcagcagga gaattgcccg atgggcgcaa attcgataac gcgagagagt
      721 tcaaggccct gctcattcag gaccaggatc gaatcgccga ggcgttcgtc gagcaactgg
      781 ccacgtacgc cttgcgacgg gtgatgacga tcgacgacgc cgagcagatc cacgcgatcg
      841 cgggagccag ccagaacgac gagtatcgcc tgcgaaccgt gatcgaaaac ttcgtccact
      901 cggacctgtt tcagaaaaga tagagccgga tcatgcctcg ctcgcttcgc tcaattcgat
      961 ttctgctcgc gactgccgcc gggctggtgc tcgccgccgg tctgaccggc cccctcgctg
     1021 cacaagaaac gggcgccgca cccgcgtacg gagaattccc ggctgccgac ggcggcaaac
     1081 ggtggatggg ggaactgatc gacgtcgatc caattaaccg ccgtggcctc attcggccag
     1141 gaggcgacct cgcaccagat cgctacaatc gctttctcgg ccagccgttc gcgatgctgc
     1201 cctatggcat gatttactat cacggcgcac cggctgaact gaaggacatc ccgctgggaa
     1261 cggtgctgca cggcgtgtta tatcagccgc cgccaggcga tgattccgtc ccgcggcccg
     1321 acccgcagga caaagtcgat gcacagcgag cgaaatactt tgtggagcac acgcacgccc
     1381 tgctcctgga ggacgacgtc agcttctatc gccgccgcgg ccaggcgtgg aaagttaccg
     1441 gtatcgaaac gagcgaaatg gacggccgcc agtttctggt cgtcacgtct gtcggcaaag
     1501 aggcacccgg cggactacac ggcgagcaga agttctgcat cgacaaaagc acgcgcgtct
     1561 ggcaggggaa ggagttcgcc ggcctgggcg aaatacaggc aggccaagcc gtgcatgtga
     1621 acctgacctg ggatcccggc tggggctatg gcaagtttca cgtgtccgac gtgtggctcg
     1681 atcagcaggc ccaggacgtg gcagccgagg tccagcggca aacgcacctt cgctaccaac
     1741 gttaccactg gccggcgggc tgggtcgatc gggttaccta cgatccgagc cggccaaacg
     1801 gcgcgggcag tgtctatgtc acgctgtccg gcggcctcga tccgtcgctc gatgaggaaa
     1861 tcattgacgc caaacgggcg gagattgcca ttgccgagcc gaccctccgc acctggcgca
     1921 aggatcatga cgcgcaaggg ggacagattc tcgaagtcaa gcgagttccc gatccgcctc
     1981 tggggagcag cggcattcag ttgcgcgttg aggtccctaa agtgccggag ggttttcgcg
     2041 acggcggaat cgtccgcgtg ggcaccacca gttttcccgc aggcatcctc ccacccgagg
     2101 agcgcgtcaa cggcttagaa gaccgctccc gctccctctc cggcagcgtt gcccccagcc
     2161 aagagtaagg catcaatcct atggccaact atctcgctca aaactggttg ctcaaccgcc
     2221 ggcacgtcct gaaggggatc ggcgtttcgc tggctctgcc gttcctggaa tgcatgcgcc
     2281 cgctgcgggc cgtcgaggcg acgccgcgag tcaagcgcag cgttttcatg tacctgccca
     2341 acggcgtcaa tccggccgat taccagatca ccagctccgg caaggactac cagctttcgc
     2401 ggtcgctgca gtcgttgaag cgtcaccggg cgaacatcac ccccatcagc ggcctgtatc
     2461 atcctcacgg tatgggcaag gcccacgatt gcagcaacat ttggctcacc ggcgcccggc
     2521 acgacgcgaa tcaccggaac acgatctcgg tcgatcaatt gatggcccgc gtgacggagc
     2581 ctcacacccg gttcccgtcg ctgcaactca gcagcgagaa ccgccgctgc ctcgcctgga
     2641 cagccggcgg cgtcgcgctg ccggacgact ccgtccccag cgtcgtcttc cgcgggctct
     2701 tcgaggagcc gaaaagtggc gtcgccgttc agcggcgcga attaaaccgc cgcggcagca
     2761 ttttggactc cgtgcttgac gaggcccgcc gtctcgacaa tcaactcggc aaccaggact
     2821 cggagcgcct ggaacaatac ctcacatcgg tccgggaaat cgaaattcgc acccagcggg
     2881 ccgatgggtg gctcgacaag ccgcggccga agatcgataa ggccagcgcg gcacagctcg
     2941 accgcgatat tccgctggaa gagctcggcg agtttctccg cacgatgtac gacctgatcg
     3001 tgctcgcatt tcagaccgat tcgacgcgcg tcgttacctt catgaccggc gacgaaggga
     3061 ccggacccgc cattccggag atcggcatca acttcggccg gcacgccctc tcgcaccata
     3121 acggcaacaa ggagatgctg gcgaatcttt cggccagcga cgcatttaac atcgaacaat
     3181 ttgcctactt cctcgatcgc ctcgccgaaa cgcgcgacgc cgaaggcccg ctgctcgaca
     3241 cgacgctggc cctctacggc agcggaatgg cctacggaca ttcgcacggc aacgccaacc
     3301 tgccgctggt gctggctggc ggcaaaagcc tgggaatcag gcacggccgg cacctcgatt
     3361 acaacgacct ggccagcccc gccgggcaca actaccaggc agaccagccc ggcgcgctgg
     3421 tgcgaatctg ccacaaaccc gtgaacccca aggcccacat gagcaatctg ctgctcacaa
     3481 tggcccaggc aatggacgta ccggctgagc aattcggcga cagcaactcg gaggcaagcg
     3541 agctgaccta ctcgcccaga ttctagcggc gtttggcgga atgccgctgc ctcagatcgc
     3601 cgacaagctc ggcagcaaca cgaatgcgat ttacaagctg ctgcatgatg cccggaagac
     3661 actgcggcgc ggtcttgagg catccggctt taccgtcgaa gacgttcgca aggcatgagc
     3721 gtaggatcca atcatggcgc taaactctgg acaagttcgc cgactgctca agatgattcg
     3781 acagacgcgc gaggcggaaa tgacctgccc ggaatgtctg gacgaactgg acaagtacac
     3841 gcaaaaaacc atcgacggta cgccgatcga cggcgtgttg gtgaatgtgc gcgaacacct
     3901 ggaagcatgc cccttctgca tggatcagtt caatctggtc ctggaagccc tccaagcgat
     3961 tgatgagttg tacgctgcgc cggtgacctc gacctcctgc agttcgcggt cactcttcgt
     4021 gctggaggtt ggaccaacaa gcgcagatta cggccggcgg aaactctcag cggtgtcctt
     4081 cgtgaagaga caccgctgca gctggcaaga tcctcgtcgc gcaagtggcc gatgcaaccg
     4141 ctacgagttg gcaatgggcg agactgcggg cggtgtgctt ctcctgcgca cgctaactgg
     4201 tgcctcaact ttgggtagct gccgattggt cggacctcgc gagcgcgcct aatggccgcg
     4261 accatttctc ggcagtatcc tggttgaaat gaccgacgtt acgcagattc tctcgcagat
     4321 cgagcagggc gacccgaacg cggccgagca actattgccg cttgtgtacg acgagcttcg
     4381 caagctggca gccgcccgca tggccgcgga ggcaccgggg caaacgctgc aggcgacggc
     4441 gcttgtacac gaagcctatt tgcggctggt agcaggcggc caaggggctg aggacttgag
     4501 gcttggggct gaagaaattc gttccgcggt gctcccccca gtaccgagcc cccagccacc
     4561 agtgcctctt tcagccttca attctcgcgg tcatttcttc gccgccgcgg ccgaggcgat
     4621 gcggcgaatc ctggtcgagc gggcccggcg cagggggcgg gtgcgccatg ggggcgaatt
     4681 ccagcggcaa ggcgcagccg aggcagaatt gtttgaggac cagcggcagg cggaaatgct
     4741 gcaggtcgat gcagccttgg atgaactggc cgaggaggat ccacgggcgg cggagctggt
     4801 caagctccgg ttcttcgtgg gaatgacgac cgccgaagcg gccgaggcgc ttggcgtatc
     4861 gacaaaaacc gttgagcggt catgggttta cgccaaggcc tggctgcggg aagcattgag
     4921 ccgctgaccg ccgtgagcga gaatctggtt ggtgcatgtc ggttttgcgg ctcaaatgtc
     4981 gcactgacag atagagggct catcacgacc gccaatctgc ggtgtttcga tgcacgagcg
     5041 cgacatcttg ctggctacgc tggaaatgcc cgggaccacg gagcgcgcgg agtatttggc
     5101 gcgggtctgc ggcatggaca ccgagctgcg cggccgcgtc gaggccttgc tgcgggcctc
     5161 cgaagtgcag gacagctttc tggaacatcc ggcagccgaa acggagctgt tcagccgcga
     5221 aacggatggc atccgccggg agatcgccgc gcacgaagaa ttgagcttgg actttctcca
     5281 gatggcggac gagccgggtg cgctaggcag gctcgggccc tatacgatca ccgagatgat
     5341 cgggcgcggc ggcatgggag tggtactgaa ggcccgcgat tccaagctca atcgcatcgt
     5401 ggccatcaag gtgctggccc ccgagttggc gtccaacccg actgctcgca agcggttcca
     5461 gcgcgaggcc catgccgccg cagccgtcgt ccatcagcat atcgtcacca tccacgccgt
     5521 cgatgaggac cggctgccgt atctggtgat ggagtgcatc gccggccagt cgctcaagga
     5581 aaagatcgac cgcgaaggcc acctgccgct tgtcgagatc ctgcgcatcg ggcagcaggt
     5641 cgcttcgggg ttggcagccg cccacgccca cggcttgatc catcgcg
//
LOCUS       DASUGB010000651         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1323474, whole genome shotgun sequence.
ACCESSION   DASUGB010000651 DASUGB010000000
VERSION     DASUGB010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323474"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1019
                     /locus_tag="VFV87_12450"
     CDS             12..1019
                     /locus_tag="VFV87_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5104619.1"
                     /translation="MLVRVLKVGVDATDREINDALYGNAPPGDKYLVLGHESFGVVEQ
                     VGPSVRRVKPGDYVTATVRRPGGSIYDLIGTNDMTSEETYYERGINLRHGFLTERFVD
                     DEEYIVRVPQGLKHLHVLMEPMSCAAKAVYQAYEAQRRMRVWRPQRAFVMGVGQIGLL
                     TALILRLRGIEVYCFARSKPGTLNSQIVQGIEAHYVSTTETPADEVVKEVGKADLIVD
                     ATGSSQVAFDAMRALGHNGVLVWTSITGANRQHEVPSDKINIDWVLGNKLLLGSVNAN
                     REHFEMGVRDLALGEMMFPGVIEKILTNPVSGLDNYKEMMRLLVEEKGTLKVFVNVAE
                     G"
     gene            complement(1175..1660)
                     /locus_tag="VFV87_12455"
     CDS             complement(1175..1660)
                     /locus_tag="VFV87_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5104620.1"
                     /translation="MTKPAKNTICLWYDREAEEAARFYARTFPDSSVGAVHRAPADYP
                     AGKKGDVLTVEFSVLGIPCIGLNGGPEVKPNWAFSFQVATADQAETDRYWNAIVENGG
                     QESMCGWCMDKWGISWQITPIALTAAVTGPDPAAAQRVFQAMMTMKKIDIAAIEAARR
                     G"
     gene            complement(1698..2258)
                     /locus_tag="VFV87_12460"
     CDS             complement(1698..2258)
                     /locus_tag="VFV87_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104621.1"
                     /translation="MKIKTADWVRSYSAGVWQVVREIPAHFEPRFSLQEPKQLYDGPL
                     FVVKRLVNDKWKRAFEIEAVHHSLLRPLNKADAKELATITKNSDLLQAFAEFDRPLSS
                     LLNIAFSLKRKTDIKKLQREISAALADELDAGVTSDRILEAIAASSFASACGAMPQSA
                     TIQCVNHDFEIRRRELIYRELRAFPF"
     gene            complement(2298..2918)
                     /locus_tag="VFV87_12465"
     CDS             complement(2298..2918)
                     /locus_tag="VFV87_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104622.1"
                     /translation="MAKRSMEDPLETIKRFLSGDLEPQAFRDALYSDDRFEALLSNDP
                     NVDSANYVMQDGGAFYFVIAQDYADPGGVLNAHGALCDFLDRNGVSYKKSNKYSDFYD
                     LVLNASPNWLHADPKFVAEHIMPEADGRTGEALRRWLSEKLLERFRCVDKPPEWIQSP
                     CWPIGKDGPLVFLGQLDVHHYFHDLATVYVFHDPSTGECTTIIQVF"
     gene            complement(3207..4343)
                     /locus_tag="VFV87_12470"
     CDS             complement(3207..4343)
                     /locus_tag="VFV87_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104623.1"
                     /translation="MSKLRSDAGGGLRVAVVGVGHLGIIHARLLAQVEGAELAAVVDP
                     SEPARTAAAAELRVPAHADHAPLLGQIDAAIVAAPTRHHHAASHDLLSHGIHVFVEKP
                     MTLNVGDADDLIATASEHDCVLQVGHVERFNPGFLAAAPHIQSPKYIDAARTSAYACR
                     STDIGVVLDLMIHDIDAILALVREDVTAVEALGAAVIGPNEDWAQARLTFAGGCVANL
                     TASRVAWQPQRSMHIVCRDCMAAIDFGSRTAKLMRPSTAVLAGELDVNALSAEERARV
                     KAHLFSDYLPLIDLPAAESNALLQEQREFVAAIRGERPVRVTGVDGRRALDVAERILA
                     QIATHRWGASADGPIGPRFETRRATLRGPHWSHQGAAVTRRLAG"
BASE COUNT          801 a         1323 c         1514 g          782 t
ORIGIN      
        1 acggccgcgg ggtgctggtg cgggtgctga aggtcggcgt cgatgcgacc gaccgcgaga
       61 tcaacgacgc cctctacggc aacgccccgc cgggagacaa gtacctggtg ctcgggcacg
      121 agtcgttcgg cgtcgtcgag caagtcggcc ccagtgtccg ccgcgtgaag ccgggcgatt
      181 atgtcacggc gaccgtccgc cggccgggcg ggtcgatcta cgacctgatc ggcaccaacg
      241 acatgaccag cgaggagacg tattacgagc ggggaatcaa cctgcggcac ggctttctga
      301 ccgagcggtt cgtcgatgac gaggaataca tcgtccgcgt gccgcagggg ctcaagcacc
      361 tgcacgtgct gatggagcca atgagctgcg cggccaaggc ggtctatcag gcatacgaag
      421 cccagcggcg aatgcgggtc tggcggccgc agcgggcatt tgtgatgggc gtcggccaga
      481 tcggtctctt aaccgccctg atcctgcgct tgcgagggat cgaggtctat tgcttcgccc
      541 gcagcaagcc aggaacgctc aacagccaga tcgtgcaggg aatcgaggcc cattacgtca
      601 gcacgaccga aacgccggcg gacgaggtcg tcaaagaggt cggcaaagcg gacctgatcg
      661 tcgatgcgac cggcagcagc caggtggcgt tcgacgcgat gcgggccctg ggccacaacg
      721 gcgtcctcgt ctggaccagc atcaccggcg ccaaccgtca gcacgaagtg ccgtccgaca
      781 agatcaatat cgactgggtg ctgggcaaca agctcctgct cggctcggtc aacgccaacc
      841 gcgagcactt cgagatgggc gtccgcgacc tcgccctcgg cgaaatgatg ttccccggcg
      901 tgatcgagaa gatcctcacc aatcccgtga gcggcctgga caactacaaa gagatgatgc
      961 ggctgctcgt ggaggaaaag gggacgctga aggtgtttgt gaacgtggcg gaggggtagg
     1021 gcagggtgca ggggacagac gacagaggac agcagaaaat ggcgctttga acggagtact
     1081 gagtactcag tacttggtac tcagtactgt ctaatcgccc cgccgcaagc cgctcgctgc
     1141 atcgctggac ataaactccg gtccctaccc ttaatcaacc ccggcgggcc gcttcgatgg
     1201 cggcgatgtc gatctttttc atcgtcatca tggcctggaa gacgcgctgg gcggcggcgg
     1261 ggtcggggcc ggtgacggct gcggtcaggg cgatgggcgt gatctgccag gagatgcccc
     1321 acttgtccat gcaccagccg cacatgcttt cctggccgcc gttttcgacg atcgcgttcc
     1381 agtagcggtc ggtttcggcc tggtcggcag tggcgacctg aaacgagaac gcccagttgg
     1441 gcttgacctc cggtccgccg ttgagcccga tgcacggaat gcccagcacg gagaactcga
     1501 ccgtcagcac gtcccccttc ttcccggccg gatagtccgc cggcgcgcga tgcactgcgc
     1561 cgaccgacga atcaggaaag gtccgggcat aaaaccgcgc cgcctcctcg gcctcgcgat
     1621 cgtaccacag gcaaatcgtg ttcttggcgg gtttggtcat gttagatctc gattggggtt
     1681 gccggctgcg gcgggtctca gaaaggaaat gctctcaact cgcgatagat cagctctcgc
     1741 cggcgaatct cgaaatcgtg gttgacacac tgaatcgtgg cagactgcgg catcgcgccg
     1801 caagcactgg caaaggacga ggcggcgatc gcctccagaa tgcgatccga cgtgactcca
     1861 gcatccagtt cgtcggcaag cgcagcggag atctcgcgct gcagcttctt gatatccgtc
     1921 ttgcgcttta aggagaacgc gatattgagt aatgatgaga gcggccggtc aaactcggcg
     1981 aatgcttgca gcaggtcgga gttcttggtg attgttgcca gttccttcgc gtctgccttg
     2041 ttcagcggcc ggaggagcga atggtggacg gcttcgatct cgaatgcccg cttccatttg
     2101 tcgttgacga gccgcttgac gacgaacagc gggccatcat aaagctgctt cggttcctgg
     2161 agcgagaacc gcggctcgaa gtgggccgga atttcgcgaa ccacctgcca aaccccggca
     2221 gaatacgatc gaacccagtc cgcggtcttg attttcatga ctgcgatcgt actacaggca
     2281 agtcgtgttc gccgttgtca aaagacttgg atgatcgtcg tgcactcacc ggtgcttggg
     2341 tcgtgaaaga cgtataccgt tgcaaggtcg tgaaagtagt gatggacgtc caactgcccg
     2401 aggaaaacca gggggccatc cttgccgatt ggccagcaag gtgattgaat ccactccggg
     2461 ggcttgtcca cgcatcgaaa gcgttccagg agcttttccg aaagccatct gcggagtgct
     2521 tcgcccgttc gtccatcggc ctcgggcatg atgtgttccg cgacgaactt ggggtccgca
     2581 tgcagccagt tcggcgacgc attcaatacc aggtcgtaga aatcggagta cttgttcgat
     2641 ttcttatagg aaacgccgtt gcgatcgaga aagtcacaca gggcgccatg cgcattgagc
     2701 acgccgcccg ggtcagcata gtcttgggcg ataacgaagt agaacgcgcc gccatcctgc
     2761 atcacgtagt tcgccgaatc gacgttcggg tcgttggaga gcagagcttc gaagcggtca
     2821 tccgagtaca acgcatcgcg aaaggcctgc ggctccaggt cgccggatag gaatctcttg
     2881 attgtttcga gaggatcttc catcgatcgc ttggccaact ggggaatgga cgatcccgcg
     2941 gggtcctggc cattttctgc ccactgtgct gcatggtcaa ttcagaatga cgcaggtgca
     3001 tgaggccgct gcggctgaaa tgcaccactc gcatcacgtg gtgcattgcg cttcgctgat
     3061 gcaccctacg gggctctgcc tctggagaac cggcgggccg cgcggcagac gccagcgccg
     3121 aactgcggga ctccctcgct ggcgcgtcgg gttggtgcgc ggggacgggt tggaaacccg
     3181 tcctacgatt cgagccgcgt gtccgcttag ccggcgaggc ggcgggtgac ggcggcgccc
     3241 tgatgcgacc agtgggggcc gcggagggtg gcgcggcggg tttcgaagcg cgggccgatg
     3301 gggccgtcgg cagaggcgcc ccagcggtgg gtggcgatct gggccaggat ccgctcggcg
     3361 acgtcgaggg cccggcggcc gtcgacgccg gtgacgcgca ccggccgctc gccgcggatg
     3421 gcggccacga actcgcgctg ctcctgcaag agggcgttgc tctctgccgc ggggaggtcg
     3481 atgagcggca gatagtcgct gaataggtga gctttgacgc gggcccgctc ttcggccgag
     3541 agcgcgttca catcgagctc gccggcgagg accgcggtgg aaggccgcat cagcttcgcc
     3601 gtccgcgagc cgaagtcgat cgccgccatg cagtcgcggc agacgatgtg catcgagcgc
     3661 tgcggctgcc aggcgacgcg cgaggcggtc agattggcga cgcagccgcc ggcgaacgtc
     3721 agccgggcct gggcccagtc ctcgttggga ccgatcacgg cggcgccgag cgcctcgacc
     3781 gccgtcacgt cctcgcggac gagggcgagg atggcgtcga tgtcgtgaat catcaggtcg
     3841 aggacgacgc cgatgtcggt cgagcggcag gcataggcgc tggtccgggc agcgtcgatg
     3901 tacttgggcg actgaatgtg cggggcggcg gccaggaagc cggggttgaa gcgttcgacg
     3961 tggccgactt gcaggacgca gtcgtgctcg ctggcggtgg cgatcaggtc gtccgcatcg
     4021 ccgacgttca gcgtcatcgg cttttcgacg aagacgtgaa tgccgtgcga gaggaggtcg
     4081 tggctggcgg catgatgatg gcgcgtcggc gcggcgacga tcgcggcgtc gatctggccg
     4141 aggagcggcg cgtgatccgc atgcgctggc acgcgcagct cggccgcggc ggccgtgcgg
     4201 gctggttcgc tgggatcgac gacggcggcc agctccgccc cttcgacctg cgcgagcagg
     4261 cgggcgtgaa tgatgcctaa atggccgacg ccgacgacgg caacgcgaag cccgccgcca
     4321 gcatcactgc ggagcttgct cacgcggccc tcctgcggtc gcgcccgcgg ccgttgcggc
     4381 cctcgtgctg gtgctgcagg aagctgagca ggtgattgac
//
LOCUS       DASUGB010000652         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_907205, whole genome shotgun sequence.
ACCESSION   DASUGB010000652 DASUGB010000000
VERSION     DASUGB010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907205"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..509
                     /gene="rnpA"
                     /locus_tag="VFV87_12475"
     CDS             153..509
                     /gene="rnpA"
                     /locus_tag="VFV87_12475"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5104624.1"
                     /translation="MPDERFGNEYRLRKAAEFDRVYKARVFAADDVLVVNGVASDLPH
                     CRLGLSVSRKVGGAVVRNRWKRLIREAFRTSKENLPAGIDLVVRPQKGAEPELAAIRR
                     SLPALARRIARRVRKM"
     gene            573..809
                     /gene="yidD"
                     /locus_tag="VFV87_12480"
     CDS             573..809
                     /gene="yidD"
                     /locus_tag="VFV87_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5104625.1"
                     /translation="MWLLHQIARLPARLLILLVRIYQFTLSPIFGRQCRFQPTCSHYM
                     IGAIEKYGALRGTLKGLYRILRCNPFCQGGYDPP"
     gene            905..2320
                     /locus_tag="VFV87_12485"
     CDS             905..2320
                     /locus_tag="VFV87_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104626.1"
                     /translation="MFRPCFSRVSIVLLATLVPTSLLGQATGPPIRVQTQTVPRQPPP
                     RTPPAGPTAEDLLRMLFPPGSQGDDFAPSGQYVPQPSAQVASPPAPRIPSAAEVASMS
                     SHQQRAELRQALARLDEELDRLPTGAAWKVYLQTSALGSALWSRPESEPEPDADTRDI
                     LRDIAERYEQVQKEPSYGAVSRLTGFQLARAILPEYATSQMEKSQRSLRTSLATFSSS
                     LSRLSTAAAWAEHLKTEELQRLASRSKELEPADTEIIRAIAERYDQAQRNPAYRQVTS
                     SAGFAESREFLQQLAEALAKQQSAAVETKRGDVAKARPDASHLERLVVGLLNEQQRLH
                     DHCAEEMRAREALSNHKLARDAPGAGDTLELVRKTQEAEDILRAIKERHEASARAADG
                     KLLKLLKALQAGSTKKVSGQTVMLLPLDASPPDDSEVVMLLQFKDEEPADTEYMLLPL
                     GTDPPKDKSDEESEKDKNQDK"
     gene            2486..>2828
                     /locus_tag="VFV87_12490"
     CDS             2486..>2828
                     /locus_tag="VFV87_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104627.1"
                     /translation="MTERAGLRCLFSPHVAFCVLMAGLLGGLFVACPASAQVITTTQR
                     PPQSAPHEGYFNCFGSFYEGDFLDAGRDFRDAGRGGIASTEGRWIDSICYYTMMGECY
                     YQMGKMGDALDQ"
BASE COUNT          525 a          932 c          901 g          470 t
ORIGIN      
        1 gctgacgtgg ctgggggccc gcgttgtcca gcgctaccgc aaagggactt cctatttccg
       61 ccctacgcag cgtcccggcg aacatgattg ggcccgcaag cctctttccg agcgaaaaga
      121 acctcctgaa ccctgaaccc gaaccctgcc ccatgcccga cgagcgcttc ggcaatgaat
      181 atcgcctccg caaggcggct gagttcgatc gggtttataa ggcgcgcgtt ttcgccgcgg
      241 acgacgtgct ggtcgtcaat ggcgtcgcca gtgacctgcc gcactgccgg ctggggctga
      301 gcgtttctcg gaaagtcggc ggggccgtcg tccgcaaccg ctggaagcgg ctgatccgcg
      361 aggccttccg cacctcgaag gaaaacctcc ccgccggaat cgacctcgtc gttcgcccgc
      421 agaagggagc cgagccggag ctggccgcca tccgccgctc gctccccgcg ctcgcccggc
      481 ggatcgcccg ccgcgtgcgt aagatgtagg aggggtttcc aacccgtccg cgctatcgca
      541 aaggacaggt tggaaacctg tcctacgaac ctatgtggct tctgcaccag atcgcccgcc
      601 ttcctgctcg cctgctgatc ctgctggtgc gcatctacca attcacgctc agcccgattt
      661 tcggccgcca atgtcggttt cagccgacgt gtagccacta catgatcggc gcgatcgaga
      721 aatacggggc ccttcgcggc acgctgaagg gcctctaccg cattctccgc tgcaacccgt
      781 tttgccaggg agggtacgac ccgccgtagg attcgtcgta caatgagcag gagcggaacg
      841 gggtagatgg tgcgttcgcc tatcgacagg cgcgcctccc aaactcatcc aagggagagc
      901 tccgatgttc cgtccctgct tttcgcgagt gtcgatcgtc cttttggcca cgttggttcc
      961 gacatccctg ttgggccagg caaccggccc gccgatccgc gtccagacgc aaaccgtgcc
     1021 gcggcagcca ccgccacgca cgccgcccgc gggtcctacg gcggaggacc ttctgcggat
     1081 gctctttccg cccgggtcgc agggggacga cttcgccccc agcggacagt acgtgccaca
     1141 gccctccgcc caggtggcga gcccgccggc cccacgcatc ccgtctgccg cggaggtcgc
     1201 cagcatgtcc agccaccagc agcgcgcgga gctgcgccag gcgctggcca ggctcgatga
     1261 agagttggat cgcctgccga ccggcgcggc gtggaaggtc tatctgcaga cgagcgcgct
     1321 gggcagcgcc ctgtggtccc ggccggaatc ggagccggag cccgatgccg acacgcgcga
     1381 catcctgcgg gacatcgccg agcggtacga gcaggtccag aaggagccct cgtacggagc
     1441 ggtgagccgc ctgacgggat tccagctcgc ccgcgcgatt ctgccggagt atgcaaccag
     1501 ccagatggaa aagagccagc gcagcctgcg gaccagcctg gcgacgttct ccagttcgct
     1561 gagccgcctg agcactgccg ccgcgtgggc ggagcacttg aaaacggaag agctgcagcg
     1621 cctggccagt cggtccaaag agttggagcc ggccgacacc gaaatcatcc gggccattgc
     1681 ggagcgatac gaccaggccc agcgcaatcc ggcgtatcgg caggtcacga gttcggccgg
     1741 cttcgccgaa tcgcgcgagt tcctgcagca gctggccgag gcactggcga agcagcaatc
     1801 cgctgccgtg gagacgaaga ggggtgatgt cgcgaaggcg cgacccgatg cctcgcacct
     1861 ggagcgcctg gtcgtgggtt tgttgaacga gcaacagcgg ctccacgacc actgtgccga
     1921 agaaatgcgg gccagggaag cactctcgaa ccacaaactg gcgcgcgatg cgccaggcgc
     1981 gggcgacact ctcgaattgg tgcgaaagac ccaggaagca gaagatattc tgcgtgcgat
     2041 caaggagcgc catgaggctt ccgcccgcgc ggcagatggc aagctgctca agctgctgaa
     2101 ggcactgcag gccggctcga ccaagaaggt ttcaggccag acggtcatgc tgctgccgct
     2161 ggatgcctcc ccgccagacg acagcgaggt cgtcatgctc ctgcagttca aggacgagga
     2221 gccagcggac acggaataca tgctgttgcc tctggggacc gacccgccaa aggacaagtc
     2281 ggacgaagaa tctgaaaaag acaagaacca ggacaaatag ggtgcccgcg cggtcgaatc
     2341 cagccccaaa cgcgacaatc cacccctgtt cgcggtatga taggacgtgc ggtcggcaca
     2401 accggctgcc taggggcaac gtaggtttac tggcgggctg cgcaggcgcg gcacgctggt
     2461 tttctctgat gccggaacca gaatcatgac ggaacgggct ggccttcgct gtcttttctc
     2521 gccccacgtt gccttctgcg tcctgatggc cggattgctg ggcggcctct ttgtcgcttg
     2581 tccggcgagc gctcaggtca tcaccaccac gcagcgcccg ccgcaatcgg ctccgcacga
     2641 gggctacttc aattgcttcg ggtcgttcta cgagggggat tttctcgacg caggccgcga
     2701 tttccgcgac gccggccgcg gcggcatcgc cagcaccgaa gggcgatgga tcgattccat
     2761 ctgctactac acgatgatgg gcgagtgtta ttaccagatg ggcaaaatgg gggacgctct
     2821 cgatcaat
//
LOCUS       DASUGB010000653         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1741497, whole genome shotgun sequence.
ACCESSION   DASUGB010000653 DASUGB010000000
VERSION     DASUGB010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741497"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFV87_12495"
     CDS             <1..476
                     /locus_tag="VFV87_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104628.1"
                     /translation="ELLPMREFRKELRGKMEQLRMDPEFGRRYVNDGFSGGEMKRAEI
                     LQLAMLQPKFAILDETDSGLDADAVRLASQSIAEIGREKMGLLIITHHDKLLEHNPPD
                     FTHVILGGKIVESSPGSTLANELHTQGYDRIRRTYPEADEANRKMVEKEEPAAVA"
     gene            529..1947
                     /gene="sufB"
                     /locus_tag="VFV87_12500"
     CDS             529..1947
                     /gene="sufB"
                     /locus_tag="VFV87_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5104629.1"
                     /translation="MATDILSPTLATDPVGEINKYDFHTESKPVFRARKGLDAQIVNQ
                     ISDMKNEPDWMRQFRLDALDIFFSKPMPKWGGSIDLNFQDIYYYLKPTDHQGKTWEDV
                     PEEIKKTFDRLGIPEAERKFLSGVKAQFESEVVYGSLKEDLAAKGVIFTDTDSAVREH
                     PDLVREYFATVIPSNDNKFAALNSAVWSGGSFIYVPKGVKIDFPLQAYFRINAERMGQ
                     FERTLIIVDEGASVHYVEGCTAPMYSTESLHSAVVEIIVKRGARCRYTTIQNWANNIY
                     NLVTKRAMAYGDATMEWIDGNLGSRLTMKYPAVYMMEPGARGEILSIAFAGAGQHQDA
                     GAKLVHVAPNTSGQIISKSISKNGGRSSYRGLVKVEKGAKNVKTNVVCDALILDKKSR
                     SDTYPYIEIAEQDVSVGHEASVSRIGEEQLFYLMSRGLSEDEASAMIVNGFIEPLVRE
                     LPMEYAVEMNRLIQLQMEGSVG"
     gene            2065..3417
                     /gene="sufD"
                     /locus_tag="VFV87_12505"
     CDS             2065..3417
                     /gene="sufD"
                     /locus_tag="VFV87_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5104630.1"
                     /translation="MPATLSKPAFDQQSFDAFLSARNEPAWLVGQRREAWQEFLEKDW
                     PQRNEEEWIRTDIRLFKLSQFGLPVDATPTRRASEGGGTPTRSVSEGVPALLTAGVDL
                     AGSSHSLDSRPAGSQLAAKWSEKGVLFGSLDELVHTHGDLVRKHIYRAVDPHFDKFSA
                     LQAACWSGGHMLYVPRGVVIDQPLHALSAMSHGGVDLGHTLVVLEEGAEATLMCETAS
                     DAQPDGGLSCSATEVILAPNSRLRLVTLQNWGHGVWNFGHQKAIVGRDSALQWTIAAI
                     GSRLSKVNQHVELAGDGAESQVNGVLFTEGKQHLSYHTLQHHAAPSCRSDFLYKSALQ
                     DQSRTVWRGMIKVDLAAVKTDGYQRNDNLLLSSTARADSIPGLEIQCDDVRCTHGSTT
                     GKVDEELIFYAMCRGLTRKEATRLIVSGFFQQIFDRITIESVREALGQAILRRVREYL
                     "
     gene            3581..3919
                     /locus_tag="VFV87_12510"
     CDS             3581..3919
                     /locus_tag="VFV87_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104631.1"
                     /translation="MSHFLKSLLRKYPNIWGAYEVFQRLERWPRIGEAATLAVDAAKQ
                     WQLELEELKRIQDRTTDWPFSLQSKWHEHWNKYGSKRDAESAVNDVIDRALQLCNASI
                     ASGNPIEFYW"
     gene            3926..4753
                     /locus_tag="VFV87_12515"
     CDS             3926..4753
                     /locus_tag="VFV87_12515"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5104632.1"
                     /translation="MSNFVRVAKTSDLADSGRTLLEVDDRIVALFRVGGEYFCIDDVC
                     THDGGPLSDGKLENHSIACPRHGAKFDIRTGKALTMPATVDTAAHEVKVEGENILVRL
                     CQPGELAGQRAAAPAAPASRPPGERGDETATARAPSPDALVAVVIEQALNPAAEEGKT
                     DEPSAQPPAARELSEDLVREELKKVVDPELFVNIVDLGLIYNVDLLPAADGKTNCKIE
                     MTMTSPMCPAGPQLIANSKQVLGALPGMGNVEVKIVLDPPWTPDKMTDEARDQLGIF"
     gene            complement(4750..>5356)
                     /locus_tag="VFV87_12520"
     CDS             complement(4750..>5356)
                     /locus_tag="VFV87_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104633.1"
                     /translation="ATKNFPGKIRWWAETVEVVLVLILIPSAMIFDFTAPARFQEEWR
                     ANRDAALDREIERREADHADFERRNQIQRDFLNEWANSRTSGNDDLHEGTTQVLIDRL
                     ETRLIFPTVLHDFILQTDSESRSEILDLTRSLSDPYRSLENLGDRGAEAERFLPILDR
                     IGASDPHPTVAKSAKKSAQWIRQRVQEDNKLPPSARRERFG"
BASE COUNT         1133 a         1694 c         1592 g          937 t
ORIGIN      
        1 aggaactgct gccgatgcgc gagttccgca aggagctgcg cggcaagatg gagcagcttc
       61 gcatggaccc ggagttcggc cgccgctacg tcaacgacgg cttctccggc ggcgagatga
      121 agcgggccga aatcctgcag ctcgccatgc tgcagccgaa gttcgcgatc ctcgacgaaa
      181 ccgacagcgg cctcgatgcc gacgccgtcc gcctggccag ccagtcaatc gccgaaatcg
      241 gccgcgagaa gatgggcctc ttgatcatca cgcaccacga caagctgctg gagcacaatc
      301 cgcccgattt cacgcacgtc atcctcggcg ggaagatcgt ggaatcgagt ccaggcagca
      361 cattggccaa cgaattgcac acccaaggct acgaccgcat ccgccgcacc tatcccgaag
      421 ccgacgaagc caaccggaag atggtcgaga aggaagaacc tgcggccgtc gcgtagggtg
      481 ggacaaggcc gccgcggccg caggcccacc gcacacaaca aaattaccat ggcaaccgac
      541 attctttcac cgacgctcgc caccgacccg gtcggcgaga tcaacaaata cgacttccat
      601 acggagtcta agccggtctt tcgcgcgcgg aaggggctcg atgcccagat cgtcaatcag
      661 atcagcgaca tgaagaacga gccggactgg atgcgccagt tccgcctcga cgcgctcgac
      721 atcttcttca gcaagccgat gcccaagtgg ggcggctcga tcgatctcaa cttccaggac
      781 atctattatt acctcaagcc gaccgaccac cagggaaaaa cctgggagga tgtgcccgag
      841 gagatcaaaa agacgttcga ccggctgggc attcccgaag cggaacggaa gttcctctcg
      901 ggcgtgaagg cccagttcga gagcgaagtc gtctatggct cgctgaagga agaccttgcg
      961 gccaaggggg tgatcttcac cgacaccgat tcggcggtcc gcgagcatcc ggacctggtg
     1021 cgcgagtact tcgccacggt catcccgtcg aacgacaaca agttcgcggc cctcaattcg
     1081 gctgtctggt cgggcgggtc gttcatctac gtgcccaagg gggtgaaaat cgacttcccg
     1141 ctccaggcct atttccgcat caatgccgag cggatggggc agttcgagcg gacgctgatc
     1201 atcgtcgatg aaggggccag cgtgcactac gtcgagggct gcaccgcccc gatgtattcg
     1261 acggaaagcc tgcactcggc ggtcgtcgag atcatcgtca agcgcggggc tcgctgccgc
     1321 tacacgacga tccagaattg ggccaacaac atctacaacc tggtcaccaa gcgggcgatg
     1381 gcctacggcg acgccacgat ggagtggatc gacggcaacc tcggcagccg gctgacgatg
     1441 aagtacccgg ccgtctatat gatggagccc ggcgcccgcg gcgagatttt gtcgatcgcc
     1501 tttgccggcg cggggcagca ccaggacgcc ggggctaagc tcgtccacgt cgctccgaac
     1561 accagcggcc agatcatcag caagtcgatc agcaaaaacg gcggccgcag cagctaccgc
     1621 ggcctggtca aggtcgaaaa gggggcgaaa aacgtcaaga cgaacgtcgt ctgcgacgcc
     1681 ctgatcctcg acaagaagag ccgcagcgac acttacccct atatcgagat cgccgagcag
     1741 gacgtgtccg tcggccacga ggccagcgtc tcgcggatcg gtgaggagca gctcttctac
     1801 ctgatgagcc gcggcctttc ggaggacgaa gccagcgcga tgatcgtcaa cggcttcatc
     1861 gagccgctcg tccgcgagct gccgatggaa tacgctgtcg aaatgaaccg gctgatccag
     1921 ttgcagatgg aaggttccgt ggggtaggcg tctcgcctgc tcgcgctgta gggaacgccc
     1981 tccgtggcgt tccgtgaact gagaatcgac gctccttcgc cgccgctcgg aacggcacag
     2041 aggccgttcc ctacagaaac atccatgccc gccacgcttt ccaaacccgc ttttgaccag
     2101 caatcgttcg acgcttttct gagcgcccga aacgagcccg cgtggctggt cgggcagcgc
     2161 cgcgaagcgt ggcaggagtt cctcgaaaag gactggcccc agcgcaacga agaagagtgg
     2221 atccgcaccg acatccggct gttcaagctg agccagttcg ggctgccggt cgatgccaca
     2281 ccaacccgac gcgcaagcga gggcggaggc acaccaaccc gaagcgtcag cgagggagtg
     2341 cccgctctcc tcaccgcagg cgtcgatctc gccggcagct cccattcgct cgatagccgg
     2401 ccggccggct cgcagctcgc tgcgaaatgg tccgaaaagg gcgtcctctt tggcagcttg
     2461 gacgagctgg tccacacgca cggcgacctg gtccgcaaac acatttaccg cgcggtcgat
     2521 ccgcacttcg acaagttctc cgctctccag gccgcctgct ggtcgggcgg gcatatgctc
     2581 tatgtccccc gcggcgtcgt catcgaccag ccgctgcatg ctctttcggc catgagccac
     2641 ggcggcgtcg atctgggcca cacgctggtc gtcctcgaag agggcgcgga agcgacgctc
     2701 atgtgcgaga ccgccagcga cgcccagccc gacggcggcc tgtcgtgcag cgcgaccgag
     2761 gtgatcctgg cccccaattc ccgtctgcgg ctggtgacgc tgcagaactg ggggcacggc
     2821 gtttggaact tcggccacca gaaggcgatc gtcggccgcg actccgccct ccaatggacc
     2881 atcgccgcca tcggcagccg gctgtcgaaa gtgaaccagc acgtcgagct ggccggcgac
     2941 ggggccgaga gccaggtgaa cggcgtcctc ttcaccgagg ggaagcagca cctttcgtat
     3001 cacacgctcc agcaccacgc tgccccaagc tgccggagcg atttcctcta caaatccgcc
     3061 ctgcaggacc agtcgcgcac cgtctggcgc gggatgatca aggtcgatct ggctgccgtc
     3121 aagaccgacg gctaccagcg gaacgacaac ctgctcctgt cgagcacggc ccgggccgat
     3181 tcgattccgg gattggaaat ccagtgcgac gacgtccgct gtacgcacgg ctcgaccacc
     3241 ggcaaggtcg atgaagagct gatcttctac gccatgtgcc gcggcctgac ccgcaaagaa
     3301 gccacccgcc tgatcgtcag cggctttttc cagcaaatct ttgaccggat tacgatcgag
     3361 agtgtccgcg aggcgctggg acaggcgatt ctgaggcgtg tccgagagta tttgtagtgc
     3421 aggcgatgct cccatgggca ggtgggtaga agttcgatgc agctgcccta accgcgggcc
     3481 tgtgaatcag cccgaccacg ggaatacgag ggtacttatt tacccctgtg gtcacaagga
     3541 aggaaccctg gcccaaacat cgccaaacga attctttgcg atgtcacact ttctgaagag
     3601 ccttcttcgc aagtatccaa acatctgggg cgcgtacgaa gtttttcaaa ggctggagcg
     3661 ttggccgcgg ataggtgaag ccgccacctt ggcggttgac gcagcaaaac agtggcaact
     3721 tgaacttgag gagctcaagc gcatccagga tcgaaccacg gattggccgt tctcgctgca
     3781 atcgaagtgg catgagcact ggaacaagta tggatccaaa cgcgatgcgg aaagcgcggt
     3841 gaatgacgtg attgaccgcg cactccaact ttgcaacgcc tcaattgcca gcggcaatcc
     3901 tatagaattc tattggtagt gatttatgtc caacttcgtc cgcgtcgcca aaacgtccga
     3961 ccttgccgat tccggccgca cgctgctgga ggtcgatgac cggatcgtcg cgctgttcag
     4021 ggtcggcggc gagtatttct gcatcgacga cgtctgcacg cacgacggcg ggccgctctc
     4081 cgacggcaag ctcgaaaacc attcgatcgc ctgcccgcgg catggcgcca agtttgacat
     4141 tcgcacgggc aaggccctga cgatgccggc caccgtcgat acggctgccc acgaagtgaa
     4201 ggtcgaaggc gagaatatcc tcgtccgcct ttgtcagccg ggcgaattgg ccggtcaacg
     4261 cgctgccgct cccgccgccc ctgcctctcg accaccagga gagaggggag atgaaaccgc
     4321 aaccgctcgc gcgccctcgc cagacgctct cgtcgcggtc gtgatcgagc aggctctcaa
     4381 tcccgccgcg gaagaaggca agacggacga accgtccgca cagccgccag ccgcgcgcga
     4441 gctgagcgaa gacctggtcc gcgaggagct gaagaaagtc gtcgatcccg agctgttcgt
     4501 gaacatcgtc gatctcgggc tgatctacaa cgtcgatctg ctacctgccg cggatggcaa
     4561 gacgaactgc aagatcgaaa tgacgatgac cagcccgatg tgccccgccg gcccgcagct
     4621 catcgccaac agcaagcaag ttctaggggc actccccggc atgggcaacg tcgaagtcaa
     4681 aatcgtcctc gacccgccct ggactcccga caagatgacc gacgaggcgc gggaccagtt
     4741 aggaatcttt tagccaaacc gttcgcgtcg cgcactcggt ggcagcttgt tgtcttcctg
     4801 aacccgctgc cgtatccact gtgctgattt cttagccgat ttagctacag tagggtgcgg
     4861 gtcgcttgcg ccaatccgat cgaggatggg caaaaatcgc tctgcctcgg cacctcggtc
     4921 accgagattc tcaagacttc ggtatggatc ggagagcgat cgcgtcaagt caagaatctc
     4981 gctacgagac tctgaatctg tttgcaaaat aaagtcgtga agaacggtcg gaaatatcaa
     5041 gcgggtttca agccggtcga taagcacctg tgtcgttcct tcgtggagat catcattgcc
     5101 cgacgtcctt gaattggccc attcgttgag aaagtcacgt tggatctgat tgcgtcgctc
     5161 gaaatcagca tggtcggctt cgcgccgctc gatttctctg tccaacgcgg catcccggtt
     5221 cgcccgccat tcttcttgaa atctggccgg agctgtgaaa tcaaagatca tcgccgaggg
     5281 aatgagaatc agtacgagca caacctcgac tgtctcagcc caccaacgga tttttccagg
     5341 aaagttcttg gtcgcc
//
LOCUS       DASUGB010000654         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_768264, whole genome shotgun sequence.
ACCESSION   DASUGB010000654 DASUGB010000000
VERSION     DASUGB010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768264"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFV87_12525"
     CDS             complement(<1..230)
                     /locus_tag="VFV87_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104634.1"
                     /translation="MPAASIWNQGAGRMALTFVVAVMLLAADEPSPDAAVEKSRQEQV
                     SVLAPAQARSLKMLSGEDRSTEAKLHSEPLLR"
     gene            complement(246..1085)
                     /locus_tag="VFV87_12530"
     CDS             complement(246..1085)
                     /locus_tag="VFV87_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104635.1"
                     /translation="MMTRRWTICCSLVCSAALSASPLAGQEPEPELTPVDKQQIEAAL
                     KLTKAEAAKYVFEIGKSDAKPELQPEPLLRWSNPSVGEVHGYVFLWTVNERPAVVGSL
                     FKWFTPHTHMSHEFHSLSEAPLSGKYDGAEKWKPRSGGVAFARLPNAPALAATPALRL
                     LQMKRFAKEFAATKKERDDSRQELRLLTQPIYRYADPKGEIADGGLFAFVQGTDPELF
                     LLLEARGKGEAASWQFAAARMNGVGFDLRYQDKAVWSVEIMPGREIYSHLEPYTSFMF
                     NMP"
     gene            complement(1082..3112)
                     /locus_tag="VFV87_12535"
     CDS             complement(1082..3112)
                     /locus_tag="VFV87_12535"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX5104636.1"
                     /translation="MSWLLIVGLKNAILVVPLAGLALGVGRFSKRPALAHALWLIVLV
                     KLLTPPIVDVPVGWRIDIELWLAANAETPQAIAEGDKIAPVYNCPDPAAQADVPGDVM
                     PVAAPAANMQDMVACDPPAQILPASEPRRAIAPAAVPSARSAIASPALWISLGAAVWL
                     LGSAFVVTVIAIRAWRFQRFVRLAARIDDRVAQRVARLARSVGLRCAPRVVVVEGVVS
                     PMLWGLGSRTRLVFPAQLAERLSEAEIDSLLLHELAHFARGDHWVRLLELATHVFYWW
                     HPVVWWSRREIEASEEQCCDAWVIQHQHGSRRSYAEALLTTIDFLCEEQPALPPVACG
                     LGDVPLLRSRLTQIMQGQLAAGLSRGVQALLLGIGIIVSPLQPALWATSSSPRAPQKP
                     VEAAPPVSDGPPAESGPAEPSSAPLPPEPRRAEGSPALAEPIAAAPSILAPLRWTAVS
                     PDGRFRLESRRDMSVRLVRQGAVWPMDFSTYQIRCAAFAPDSKSFITGHADGTVRWWD
                     CETGGHLNTYGDKSAPVTSVAFGPDNRIAMGSANGTLAVWNLATSEETQLTGLPPVAC
                     VRWSPESGRLAVGLGSYQSSEGTVLLWDPRTSAEPAEHWLPQPVGALNWLNESSLLVA
                     NWSGQCQMLDAASGELSPSVKLDKTLVSAAAWSPDCPLVSAWHRQQLARGAE"
     gene            complement(3189..3593)
                     /locus_tag="VFV87_12540"
     CDS             complement(3189..3593)
                     /locus_tag="VFV87_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5104637.1"
                     /translation="MPRAAQDVTDAELAILQELWDRGSATVRELAERLYQKSEPSQHA
                     TVQKLLERLEGKGCVARDRQTWPHTFSAVVERNVLIGRQLQQTADKLAGGSLQPLLTH
                     LVQAGRLSAEDRKSLRALLDQLNEPEKKQGKR"
BASE COUNT          679 a         1241 c         1251 g          619 t
ORIGIN      
        1 cagcgcagga gcggctccga atgcagcttc gcttccgtcg agcgatcctc gcccgaaagc
       61 atcttcagcg aacgggcttg cgcaggcgcg aggacggaca cttgttcttg gcgggacttt
      121 tcaacggcag cgtccggcga aggctcgtcg gctgcgagaa gcattaccgc gacaacaaac
      181 gtcaaagcca tccgtcctgc cccctgattc cagatcgatg cggcaggcac gatgcctgcc
      241 ccgggttacg gcatgttgaa catgaagctt gtatagggct ctaggtgact gtagatttcg
      301 cgaccaggca tgatctcgac cgaccagacc gccttgtcct gataacgaag gtcaaagccg
      361 acgccgttca tccgcgcggc ggcgaattgc cagcttgccg cctcgccctt gccgcgggct
      421 tccagcagca ggaacaactc ggggtcggtt ccctggacga acgcgaacag gccgccgtcg
      481 gcgatctcgc ccttgggatc cgcgtagcga taaatgggct gtgtgagcag gcgcagctcc
      541 tgccggctgt cgtcgcgctc cttcttggtc gcggcaaatt ccttggcaaa ccgcttcatc
      601 tgcagcaatc gcaaggcagg agtagcggcg agagcgggag cattgggcag gcgggcaaac
      661 gccacgccgc ccgagcgcgg tttccacttc tcggcgccgt catatttgcc gctgagcggc
      721 gcttcggaca gcgagtggaa ctcgtgcgac atgtgcgtgt gcggtgtgaa ccatttaaag
      781 agggagccga cgaccgccgg ccgctcgttc accgtccaca ggaatacgta gccgtgaacc
      841 tcgccgaccg acgggttcga ccagcgcaac agcggctcgg gttgcagttc cggcttcgcg
      901 tcggatttgc caatctcaaa gacgtatttc gcggcctcgg ctttggtcag cttgagggcc
      961 gcttcgatct gctgtttatc gacgggcgtg agctcgggtt cgggctcctg gccggcgagc
     1021 ggcgaggcac tcaaagcagc cgagcaaacg agcgaacaac agatcgtcca gcggcgtgtc
     1081 atcattccgc gccccttgca agctgctgcc tgtgccacgc ggagacgagc gggcagtcgg
     1141 gcgaccaggc ggcagcggaa acgagcgtct tatcgagctt gacggagggc gacagctcgc
     1201 cactggcggc atccagcatc tggcattggc cgctccagtt ggcgacgagg agcgacgatt
     1261 cgttcagcca attgagcgct ccgaccggct gtgggagcca atgctcggcg ggctcagccg
     1321 acgtgcgcgg atcccacagg agaaccgttc cctcgctcga ctgatagctg cccagtccga
     1381 cagccagccg gccgctttcc ggcgaccagc ggacacaggc gactggaggc aatccagtca
     1441 gttgtgtctc ttcgcttgtc gccagattcc acacggctag cgttccgtta gctgaaccca
     1501 tggcgatccg attgtccgga ccgaaggcaa ccgaggtaac gggggccgat ttgtcgccat
     1561 aggtattcaa gtgcccgcct gtctcgcaat cccaccagcg gaccgtgccg tcggcatggc
     1621 ccgtgataaa cgacttgcta tcgggcgcga acgcggcgca gcgaatctga tacgtgctga
     1681 agtccattgg ccaaaccgcc ccttggcgga cgagccgcac gctcatgtcg cggcgcgatt
     1741 ccaggcggaa gcggccatcg ggcgaaacgg ctgtccagcg gagaggagcg agaatcgatg
     1801 gggcggccgc gatcggctcc gcgagggcgg gcgagccttc cgcgcggcgt ggctcaggcg
     1861 gcagcggcgc ggaggatggc tcggccgggc cagattccgc gggcggacca tcgctcacgg
     1921 gcggcgctgc ttcaacgggc ttctgcggcg ctcgtggtga actcgaagtc gcccagagcg
     1981 cgggctgcag cggagaaacg atgatgccga tgcccagcag cagcgcttgc acgccgcgcg
     2041 agagcccggc ggcaagctgg ccctgcatga tttgcgtcag ccgcgagcgc aggagcggca
     2101 catcgcccag cccgcaggcc acgggaggca gcgcgggctg ctcttcgcag aggaagtcaa
     2161 tcgtggttaa cagcgcctcg gcgtaagacc ggcggctgcc gtgctgatgt tggatgaccc
     2221 aggcgtcgca gcactgctct tcggaggcct cgatctcgcg gcggctccac cagacgaccg
     2281 gatgccacca gtagaacacg tgcgtcgcca attccagcag ccgcacccaa tgatcgccgc
     2341 gggcaaagtg agccaattca tgcaacaaca gcgaatcgat ctccgcctcg ctcaggcgct
     2401 cggcaagctg cgccgggaac acgagccgcg tgcggctacc caggccccag agcatgggcg
     2461 agacgacgcc ttcgacgacg acgacgcgcg gggcgcaacg cagtcccacc gagcgcgcca
     2521 accgggcaac gcgctgcgcc acgcggtcgt cgatccgcgc agccagacgc acgaatcgct
     2581 gaaagcgcca ggcgcggatg gcaatgacag tcaccacgaa ggcgcttccc aggagccaga
     2641 ccgcggcgcc caaactgatc cacagtgcgg gcgatgcgat tgcgctacgc gcgctgggaa
     2701 ccgcggccgg cgcaatggcg cgccgcggct cgctggccgg caggatctgg gcgggcggat
     2761 cgcacgcgac catgtcctgc atgttggcag cgggcgcggc aaccggcatc acgtcgcccg
     2821 gcacatcagc ctgcgctgcc ggatccgggc aattgtaaac gggggcgatc ttgtcgcctt
     2881 cagcgattgc ctgtggcgtt tccgcattgg ccgccaacca caattcgatg tcaatccgcc
     2941 agccgaccgg cacatcgacg atgggcggcg tgagcagctt gacaaggaca atcagccaca
     3001 gggcgtgggc cagggccggc cgcttggaga aacgccccac gccgagtgcc agcccagcca
     3061 gcggcacgac gaggatcgcg ttcttcaggc cgacgatcag cagccaactc atggcgagcc
     3121 tccggcgcga gcacccctgg gggaatctgg ccaatcccaa cctagacgac tcgcgacgcg
     3181 cgggagttct atctcttacc ctgtttcttc tccggttcgt tcaactgatc caagagcgcc
     3241 cgcagcgact tgcggtcctc cgccgagagc cgacccgcct gtaccaggtg cgtcaacagc
     3301 ggctgcaggg agccgccggc gagcttgtcg gccgtttgct ggagctggcg gccgatgagc
     3361 acgttccgct cgaccacggc cgagaacgtg tgcggccagg tctggcggtc gcgggcgacg
     3421 cagcccttcc cctccagccg ttccagcagc ttctgcaccg tggcgtgctg cgaaggctcc
     3481 gacttttgat agagccgctc ggcgagctcg cgcacggtgg ccgacccgcg gtcccacagc
     3541 tcctgcagga tcgccagctc ggcatcggtc acatcttgtg cggcgcgtgg catgggatgt
     3601 tcgttctccg gggagattta ttgccatcga gcatgctaaa gacaatctgt cttgtttgca
     3661 agccccaggg gctatttttt ctcggcagcg ggcgctggat tggcctccgc ggcggtcagg
     3721 aacagccagt agggatcggc gggggtgcca acgacgccgg gcgtgcggtc ccgcttccag
     3781 acttccttgc
//
LOCUS       DASUGB010000655         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_628630, whole genome shotgun sequence.
ACCESSION   DASUGB010000655 DASUGB010000000
VERSION     DASUGB010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628630"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFV87_12545"
     CDS             <1..353
                     /locus_tag="VFV87_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104638.1"
                     /translation="LVDALGVLPAFEKISEIPGATVLANEPSIQFALDEECRLQCRLS
                     IETRTTAYHVRTREFPEEQISVYFTARKYGSLENGETFVSAMNKLAETCREVVDNYIV
                     ENVLRPLQQTIAIR"
     gene            complement(361..594)
                     /locus_tag="VFV87_12550"
     CDS             complement(361..594)
                     /locus_tag="VFV87_12550"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5104639.1"
                     /translation="MATATMNGVASCLGQIGDTAGEVWHALNDNGPMSLAKLFDRVGG
                     NRDVVMQAVGWLAREGKIDIVETKRGRIVSLRA"
     gene            complement(739..>2664)
                     /locus_tag="VFV87_12555"
     CDS             complement(739..>2664)
                     /locus_tag="VFV87_12555"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX5104640.1"
                     /translation="VIHGKIDKGDYTVERVYFESLPGFYVTGSLYRPKKAEGKVPAVL
                     TPHGHWANGRFHDAGEAAAKQQLASGAEKEAEAARSPLQARQVHLARMGCIAFHYDML
                     GYADSQQLSQSLVHGFKAQRPEMTANENWGFFSPQAESHLQSVMGLQTWNSIRALDFV
                     LSLPEVDPERIAVSGASGGGTQTMLLAAIDPRVAVSFPAVMVSTAMQGGCTCENASCL
                     RVDTGNIEFAALFAPKPQGMTAADDWTKEMSTKGFPELKVLYERLGAPENVMLHNRTE
                     FGHNYNLLSRLALYGWLNKHFRLGLAEPIEERSFERLTTEQMTVWDSKHPQPEGGDAF
                     ERKLARQLHEGAQKQLAAIQPTSEQNLAEWRKTTGGAIDAILGRGLPGAKELEFVQTE
                     TTDKGAYALMAGLLKNKRGEELPVAYLVPNDWNGRAIIWLSEKGKAALFNDDGSPSAE
                     AKKLVDSRAAVIGVDLLFQGEFLADGQPVTKNRTVNNTREFAGYTYGYNHTLLGQRAH
                     DVQTVVAFVKLVGEPPKSVEVVALDGTGPIAALALANCGGAVDRAAINTHGLRFGKLN
                     DYLDVNFLPGGAKYGDLPGMLALAAPTELWVAGETADSAALTKDAYAAAKAEGKLTLH
                     SGPADEAASAAAAWLAR"
BASE COUNT          438 a          891 c          803 g          532 t
ORIGIN      
        1 tcctggtcga tgccctgggc gtgctgccgg cgtttgagaa gatctccgaa attcccggcg
       61 cgaccgtgct ggccaacgag ccttcgatcc agttcgccct ggatgaggaa tgccgcctgc
      121 aatgccggct gagcatcgag acgcggacga cggcctatca cgtgcggacc cgcgagttcc
      181 ccgaggagca gatcagcgtt tatttcaccg cgcgaaagta cggcagcctg gagaatggcg
      241 aaacgttcgt ttcggccatg aacaagctgg ccgagacctg ccgcgaagtc gtcgataact
      301 acatcgtcga gaacgtgctc cggccgctgc agcagacgat tgcgatccgg tagctccgct
      361 ctacgcccgc agcgagacaa tgcggccgcg cttggtttcg acgatgtcga tctttccttc
      421 gcgagccagc cagccgacgg cttgcatgac gacatcccgg ttgccgccaa cgcggtcgaa
      481 gagtttggcg agcgacatcg gaccgttgtc gttcagggcg tgccagactt cacccgccgt
      541 atcaccaatc tgtcccaagc aagatgctac gccgttcatc gtcgctgtcg ccatgaaatg
      601 ccactccttt cccgccaggg gctgctcatg ccgagccgca cccggctcat tctcacgcca
      661 ttgttaacga tgagccgggc gttcgtaaag tcgcctatcg taggtgactc gctccgcgag
      721 tcgccctcga tagtccatct atcgggccag ccacgcggca gccgcgctag ccgcctcgtc
      781 ggccggtccg ctgtgcagcg tgagcttgcc ctcggccttt gccgccgcat aggcgtcctt
      841 cgttagcgcc gcggaatcgg ccgtttcacc cgccacccac agctcggtcg gcgccgcgag
      901 cgccagcatc cccggcagat cgccatactt cgcgccgccc ggcagaaagt tgacgtcgag
      961 gtaatcgttc agtttgccga aacggagccc gtgggtgttg atcgccgcgc gatcgacggc
     1021 gccgccgcag ttggctaagg ccaaagcggc aatcggcccc gtgccgtcga gcgctaccac
     1081 ctcgaccgac ttgggcggct cgccgacgag cttcacgaag gccaccaccg tttgcacatc
     1141 gtgagcccgc tgtccgagca gtgtgtggtt gtagccatag gtgtagccgg cgaactcgcg
     1201 cgtgttgttg accgtgcggt tcttggttac cggctggccg tccgctaaga actcgccctg
     1261 gaagagcaga tcgacgccga tcaccgccgc gcggctatcg acgagcttct tggcttctgc
     1321 cgagggcgag ccgtcgtcgt tgaaaagcgc cgccttcccc ttctcactca gccagatgat
     1381 cgcccggccg ttccaatcat tcggaacaag ataggccacc ggcaattctt cgccgcgctt
     1441 gttcttcagc agccccgcca tcagggcgta cgcccctttg tccgtcgttt cggtttgcac
     1501 gaactcgagt tccttcgcac cgggcagacc gcggcccaag atggcgtcga tcgccccgcc
     1561 agttgttttg cgccactcgg caagattctg ctcgcttgtc ggctggatgg cggcgagttg
     1621 cttctgggcg ccttcgtgca gttggcgggc gagctttcgc tcgaatgcgt cgcccccctc
     1681 gggctgcgga tgcttgctat cccacaccgt catttgctcg gtcgtcagcc gctcgaacga
     1741 ccgctcttcg atcggctcgg ccagccccag ccgaaaatgc ttgttgagcc agccatacag
     1801 cgccagccgg ctgagcaggt tgtagttgtg gccgaattcc gtgcgattgt gcagcatcac
     1861 gttttccggc gcgcccagcc gctcgtagag caccttcagt tcgggaaagc ccttggtgct
     1921 catttccttg gtccagtcgt cggcagcggt catgccttgc ggcttgggag cgaagagggc
     1981 ggcgaactcg atgttccccg tatcgacccg caggcaactc gcgttctcgc acgtgcagcc
     2041 tccttgcatg gccgtcgata ccatgacagc cggaaacgag acggccacgc gcggatcgat
     2101 cgcggccaaa agcatcgtct gcgtcccacc gccgctggcc ccgctgaccg cgatgcgctc
     2161 gggatcgact tccggcagcg aaagaacgaa atcgagcgcc cggatcgaat tccatgtttg
     2221 caggcccatg accgattgca ggtgggactc ggcctgcggg ctgaagaagc cccagttctc
     2281 gttggcggtc atctctggcc gttgcgcctt gaagccatgc accagcgact ggctgagctg
     2341 ctggctgtcc gcatagccga gcatgtcgta gtggaatgcg atgcagccca tccgcgccag
     2401 atgcacctgc cgggcctgca gtggtgagcg agcggcctct gcctcctttt cggctccgct
     2461 ggcgagttgc tgcttggcag cggcttcgcc cgcatcgtgg aagcggccat tggcccagtg
     2521 cccgtgcggc gtgaggaccg cgggaacttt gccttccgcc ttcttcggcc ggtacaagct
     2581 gccggtgaca tagaagcccg gcaagctctc gaaatagacc cgctccaccg tgtaatcgcc
     2641 cttgtcgatt ttgccgtgaa tcac
//
LOCUS       DASUGB010000656         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_72945,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000656 DASUGB010000000
VERSION     DASUGB010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72945"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFV87_12560"
     CDS             <1..935
                     /locus_tag="VFV87_12560"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5104641.1"
                     /translation="ATLLVGTMPAATACAAEPLVVALWPGKTPGDVGIEGEERSRTHN
                     SPLVGPTRLITNVTRPTLTVYRPPQDKNTHTAMIICPGGGYHDLYWELEGEEVAAWLN
                     SQGMTGIILKYRCPRRPGDVKGEPPLGPLLDAQRAISLVRSRAAEWDIDPRRIGMVGF
                     SAGGHLALAAATRFEQRTYQPTDASDQASCRPDFAVMCYSGYLKAKDKDEVWPGLRVP
                     AGTPPIFLAHANDDSEKVGGSIPENSAIMYLALKRAGVPVELHIFGNGNHDFGVRQND
                     KLPASWPQLCVHWLRSLDLLEPIGAAKDSAGKTP"
     gene            complement(953..1504)
                     /locus_tag="VFV87_12565"
     CDS             complement(953..1504)
                     /locus_tag="VFV87_12565"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5104642.1"
                     /translation="MPKVENRSMAEIQSSPANRCGFWLTLSRMTAIQTGDRAPDFTSE
                     AHDGRQVSLADYRGQVVVLFFYPSDGTPVCTAEACAFRDAYADFTQAGAVVMGVSADS
                     PESHRKFIADHRLPFVLLSDPVGALRKLFGVPRTLGIFPGRVTYVIDKQGVVRLVFNS
                     QLAAARHVTETLQAVHQLAAESA"
     gene            1562..>3285
                     /gene="dnaG"
                     /locus_tag="VFV87_12570"
     CDS             1562..>3285
                     /gene="dnaG"
                     /locus_tag="VFV87_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5104643.1"
                     /translation="MPSSPAERFLTRTAIQPAARRVILRQLAAFPRPEPMQESAVHSG
                     DAKEQVRSCTDIVDLAGRSIALRRQGRIYVGLCPWHDDTKPSLQVNPDRQTWKCWVCD
                     IGGDVFSFVMKQEGCDFREALTMLAERAGIQLSHQPQRKAEPGSPDDKNTLYSCCEWA
                     SRQFHDCLLKSDAAKAARDYVQERAITPASVERFNVGFAPDDWSWLVDRAKSTPYSPK
                     VLEAAGLVGRGERGYYDRFKGRLIFPIRDTQGRTIAFGGRVLPGNIDPRAGKYVNCPE
                     TRLYTKSDTLYALDVAKNHIAQSRQLIVVEGYTDVILAHQHGIGEAIACCGTAVTERH
                     IRTLKRFADTIYLVLDGDDAGQRRTSEVLELFVAAQVDLRIMTLPDELDPAEFVRERG
                     GEAFRTLLSAASDALEHKISTATRGIDLVRDTHRANQALEEILATIARGLPPGSIDSA
                     GLRAHQLLARLARQFQVSDGDLRSRFAQLRRSAKPRVDAAPSSLPEPEYKLSALSSCE
                     CELFEILDLHPELAPVALAEIADEDLVSAPAMEILAAYRRREEAGQALDFVAVLGDLE
                     NASLKSLLV"
BASE COUNT          604 a         1069 c         1059 g          553 t
ORIGIN      
        1 ttgccacgct gctggtcggt acgatgccag cagcgaccgc gtgcgccgct gagccgcttg
       61 tcgtcgcctt gtggccgggc aagacgccgg gcgacgtggg gatcgaaggc gaagagcgca
      121 gtcgcactca caattcgccg ctcgtcggac cgaccaggct cattacgaat gtcacgcggc
      181 cgacactcac cgtctatcga ccgccgcagg acaagaatac gcatacggcg atgattatct
      241 gcccgggagg cggctaccac gacctgtatt gggagctcga aggggaggaa gtcgccgcct
      301 ggttgaattc gcaggggatg accgggatca tcctcaagta ccgctgtccg cggcggccgg
      361 gggacgtgaa aggggagccc ccgctgggtc cactgctcga tgcccagcgg gccatcagcc
      421 tcgttcgttc gcgggcggcg gaatgggaca tcgatccgcg gcggatcggg atggtcggtt
      481 tctccgcggg cggccacctc gcgctggctg ctgcgacccg ttttgagcag cggacgtacc
      541 agccgaccga cgccagcgac caggccagtt gccggccgga ttttgccgtg atgtgctact
      601 cgggctactt gaaagccaag gacaaggacg aggtctggcc gggcctgcgc gtcccggccg
      661 gtacgccgcc aatctttctg gcccatgcca acgacgacag tgaaaaggtg gggggctcaa
      721 tcccggaaaa cagcgcgatc atgtatttgg cgctcaagcg ggccggcgtt ccggtcgagc
      781 tgcacatctt cggcaacggc aaccacgact tcggcgtccg gcaaaacgac aagctccccg
      841 cgagctggcc gcaattgtgc gtccactggc tccgcagcct tgacctgctc gagccgattg
      901 gtgccgccaa agattctgcc ggcaaaaccc cgtagcgcat ggcgatgcca cgtcaggcac
      961 tttctgccgc cagttggtgg acggcctgca gggtctcggt cacatgccgg gcggcggcca
     1021 gctgcgaatt gaacaccaga cgtacgacgc cttgcttgtc gatcacgtaa gtcacgcgcc
     1081 cgggaaaaat gcccagcgtt ctgggcacgc cgaacagttt tcgcagcgcc ccgaccggat
     1141 cgctcagcag cacaaagggc agccggtgat cggcgatgaa tttgcggtgg ctttcgggcg
     1201 aatcggcgct cacacccatc accacggccc ccgcctgggt gaaatccgca tacgcatcgc
     1261 gaaaggcgca ggcctcggcg gtgcagaccg gcgtgccatc gctggggtag aaaaagagca
     1321 cgaccacttg tccgcgatag tcggccagcg acacctgccg cccgtcgtga gcttcggaag
     1381 tgaagtccgg cgcgcggtcg ccggtttgga ttgccgtcat acgggacaat gtaagccaga
     1441 atccgcaacg attcgccggc gagctttgaa tttctgccat tgaccggttt tccaccttcg
     1501 gcatacgatt cagcctgtgc tatggagggc acactatcga ccgacccagt cgatggcatg
     1561 gatgccgagc tcgccggccg agcggttcct gacccggacc gcgatccagc ccgcagcgcg
     1621 gcgcgttata ctccggcaat tggccgcctt cccgcggccc gagccaatgc aggagtcggc
     1681 cgtgcatagc ggtgatgcca aggagcaggt gcgttcgtgc accgacattg tcgatctggc
     1741 cggcaggtcg atcgcgctcc gccgccaagg gcgaatctac gtcggcctct gcccctggca
     1801 cgacgacacc aagcccagtc tccaggttaa tcccgaccga cagacgtgga agtgctgggt
     1861 ctgcgacatt ggcggtgacg tcttcagctt cgtgatgaag caggaagggt gcgatttccg
     1921 cgaggccctg accatgctgg ccgagcgggc cggcatccag ctttctcatc agccgcagcg
     1981 gaaagcggag ccaggcagcc cggacgacaa aaacactctg tattcgtgct gcgagtgggc
     2041 gtcccgccag ttccatgact gcctgctcaa gtcagacgcc gccaaagccg cccgcgatta
     2101 cgtccaggag cgggccatca cgccggccag cgtcgagcgc ttcaacgtcg gctttgcacc
     2161 ggacgattgg tcgtggctgg tggaccgggc caaaagcaca ccctattcgc cgaaagtgct
     2221 ggaagctgcc ggcctcgtcg gccggggcga gcggggctac tacgaccgct tcaaggggcg
     2281 gctcatcttt ccgatccgcg acacgcaagg acggacgatc gccttcggcg gccgcgtgct
     2341 cccgggaaac atcgacccgc gagccggcaa atatgtcaac tgcccggaaa cgcggctgta
     2401 caccaagagc gacacgctct acgccctcga tgtggcgaaa aaccacattg cccagtcgcg
     2461 gcagctgatc gtggtcgaag gctacaccga cgtaatcctg gcccaccagc atggcattgg
     2521 cgaggcaatc gcctgctgcg ggaccgccgt caccgagcgg cacatccgca cgctcaaacg
     2581 gttcgccgac acgatctacc tggtgctcga cggcgacgac gccggccagc ggcggacgag
     2641 cgaggtgctg gagctcttcg tcgcggcgca ggtcgatctg cggatcatga cgctgcccga
     2701 cgaactcgac ccggctgaat tcgtccggga gcgcggcggc gaggccttcc gcacgctgtt
     2761 gagcgcggcg agcgacgccc tggagcataa aatcagcaca gccacccgcg gcatcgacct
     2821 ggtgcgcgac acccatcggg ccaaccaggc cctggaagag atcctggcga cgatcgcccg
     2881 cggcctgcca ccgggttcga tcgacagcgc cgggttaagg gcgcatcagt tgctcgcccg
     2941 cctggcacgc cagttccagg taagcgatgg cgaccttcgc agccgatttg cccagcttcg
     3001 ccgcagtgcc aagccgcgcg tggatgccgc gccctcttcg ctgcccgagc cggagtacaa
     3061 gctgtcggct ctttcatcgt gcgagtgcga gttgttcgag attctcgatt tgcacccgga
     3121 gctggcgcca gtggccttgg cggaaattgc tgacgaagac ttggtttcag cgccggcgat
     3181 ggagattctc gccgcctatc gccgccgcga agaggctggc caggcgctgg atttcgtggc
     3241 cgttctggga gaccttgaaa atgcgtcgct gaaaagtttg ctcgt
//
LOCUS       DASUGB010000657         6109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_213635, whole genome shotgun sequence.
ACCESSION   DASUGB010000657 DASUGB010000000
VERSION     DASUGB010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213635"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFV87_12575"
     CDS             complement(<1..462)
                     /locus_tag="VFV87_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104644.1"
                     /translation="MLLALAGCQATGSQQPIANFSPTASAPTTTAPATAQQGTSDANE
                     PAQQTMVQRVQFAEPLLAPAPPPDLPAPSTPEPVRSSGPTLADFEQMALAANPSVARA
                     AALVQAARGNYTQVGLPPNPSVGYEGQQLGSGGLAEQDGIFIQQEFVRGGKL"
     gene            complement(539..1681)
                     /locus_tag="VFV87_12580"
     CDS             complement(539..1681)
                     /locus_tag="VFV87_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104645.1"
                     /translation="MFKFAPYVFKGLWQHRSRTLLTVSGSAVALFVFCFVGAVQQGLA
                     RLTEDQEAMRTLIVFQENRFCPASSKLPEDYGRTVAKMPGVAEVVPIKVFTNNCRASL
                     DVIVFNGMPPEKLRGARDLTLLSGSWTEFDSRTDAAIVGQAVAQRRGIKTGQRFTIGD
                     VTVSVVGTFRSTIAAEDNYIYTHLDFLQLARGKSGLGTVTQLEVHVAPGADPDQIAGQ
                     IDAQFKTGQVATSTRSKGVFQRDTLSDLAELIGFAHWLGYACVGLVLALVATTTVMAV
                     QDRIKEHAVLQTLGVRPGRVFRLIVAESVIQSLAGGAIGIGTGLLLLTFGGFAVGAEG
                     VTIAFRPSLSLVVTGAVVSLVVGMLAGLAPGWQAARTEIVAALRQA"
     gene            complement(1684..2406)
                     /locus_tag="VFV87_12585"
     CDS             complement(1684..2406)
                     /locus_tag="VFV87_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104646.1"
                     /translation="MALVEIRRLTKQFRKGDEVITPLQEVDLEVERGDFVSLMGASGS
                     GKSTLLNALAGIDRPTSGEIVVNGTDITRLSRGQLADWRAANVGYIFQMHNLIPVLTA
                     YENVELPLLLLPMSSAERSKRVQIALEAVNLTDRSNHYPRQLSGGQEQRVGIARAIVA
                     NPTLIVGDEPTGDLDAVTTEQILDLVQRLNTELGMTLLLVTHDPKVAALAKRQLRLEQ
                     GKLIEKGREVSVVGVEAIERRA"
     gene            complement(2461..4041)
                     /locus_tag="VFV87_12590"
     CDS             complement(2461..4041)
                     /locus_tag="VFV87_12590"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5104647.1"
                     /translation="MSTPVDLRQLAVERPVAPSHTLVKKRAWLTRWGIPSAIAGGFLV
                     LTGWSARDWFLPAKEVTVTPVILAKAEVQQAGTPLFQAAGWIEPRPTAVMASALVEGV
                     VDKLLVIEGQEVTAGQPVAKLIDAEARLAAREANVALRLRRSELPVAEAGLEAAQATF
                     DQPVQLEAAHAEAEAELARLNTEIKNLPFLIKAAEARLTFAEQELTRKKAVADAVAAR
                     VVQLAQSEFDSATAALQELKERGPTLEIQQKAWQRKCDALHTRWKLKTEETRALAEAT
                     AQVESAKTRVEQAELVVEAANLRLERMTIRAPITGRVLALHSRPGQRLMGMNAASERD
                     ASAVVSLYDPAQLQVRADVRLEDVPQAQMGQPVQIQTAASPQPLAGKVVAITSQADIG
                     KNTLQVKVSIDEPPAVIKPDMIAQVTFLAPQIPGRSTDSENDPLRMLVPRELVEKTEN
                     EAVVWVTDPATSVARRRVVQLGQAGTSQLVEVAQGLSPLDKLIVSGREGIADGTRVRV
                     TGEDRSLGKDSPSPTPAP"
     gene            complement(4066..4593)
                     /locus_tag="VFV87_12595"
     CDS             complement(4066..4593)
                     /locus_tag="VFV87_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104648.1"
                     /translation="MSKYHLTILSALLAAIIPLTGCGASATAPANNSASAEGSAYRLD
                     VEPADAKDVKAAKEVIKDEEEITLVGRVGGDVNPWIEGQAAFLIVDRALKPCNEKDDD
                     ACPTPWDYCCDADSLPEHKAMVKVVDGSGKTVSTDARKLLGLKELQTVVVHGKAKRDD
                     AGNLTVLADGVFVRN"
     gene            complement(4617..5786)
                     /locus_tag="VFV87_12600"
     CDS             complement(4617..5786)
                     /locus_tag="VFV87_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104649.1"
                     /translation="MLRTRLLPWEYGIRNLFRRPARSALTLAALSTVVLLVLVVVAFI
                     RGLETSLAVSGEPNVVLVYSIGAAADIENSAIPAGTASLLSASLDGIDRRFGLTYTSP
                     ELYVGTKVLTDPNSPGMFGIVRGVTPTAPLLRRQVRLLEGNWPGTNEVLVGRLAASKL
                     GCDPEKLQIGNTVRFDGQEWRICGRFAAGGAAFESEIWAALADLQSALKRQDVSLVAV
                     GLANGASPSDIELFCKERFDLELQALSETSYYVSLQKHYQPVRMLGWLVVLLVAGAGI
                     FAGLNTMYGAVVGRVRELSTLQAVGFRRRAIVISLIQEATLLSMAASLLAAAVAIVLV
                     NGTAVRFTMGAFALRVDGVAILVGCGTGFLLGFVGAIPPAIKAMRASVVEGLKAV"
     gene            complement(5923..>6109)
                     /locus_tag="VFV87_12605"
     CDS             complement(5923..>6109)
                     /locus_tag="VFV87_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104650.1"
                     /translation="FDATARGPAAPLLDASLWAPAVDSAIELELTSADWLTRPAGPPP
                     PLRDGRQVRIAVQSFLL"
BASE COUNT         1110 a         1970 c         1887 g         1142 t
ORIGIN      
        1 taacttgccg cctcggacaa attcctgctg aatgaagatc ccgtcttgtt ccgcgaggcc
       61 gccgcttccc agctgttgtc cttcgtaacc aaccgacgga ttgggaggca gaccgacctg
      121 cgtgtagttt cctcgagccg cttgcactag cgctgccgcc cgagccaccg aggggttggc
      181 ggccagggcc atttgctcaa aatcagcgag cgtcggaccg ctactgcgta ccggctccgg
      241 ggttgatgga gcaggtaaat ccggtggcgg ggcgggtgcc agaagcggtt ctgcgaactg
      301 gacgcgctgg accatcgttt gctgcgccgg ctcatttgcg tccgacgtac cctgctgcgc
      361 ggtggcaggg gcagttgtag tcggcgcgct cgcggttggc gagaaattgg caatgggctg
      421 ctgggatccg gtcgcctggc atcctgccag ggcgagcagc acgagtatgt agttccaacg
      481 catgcgagcc tccgtgctat tccattctcg ttcagcggcc gatagcggcc cccagatatt
      541 acgcttgccg caatgccgcc acgatttccg tccgggccgc ctgccagcct ggggccagcc
      601 cggcgagcat cccgaccacc agcgacacga cagctccagt tacaaccaga ctcagcgatg
      661 gccgaaaggc gatcgttaca ccctccgcgc cgacggcgaa tcccccaaac gtcagcagca
      721 gcaagccggt gccgattcca atcgcaccgc ctgccaaact ctgaatcacg ctttccgcca
      781 cgatcagccg aaagactcgg ccgggccgta cgccaagcgt ttgcaggact gcatgctcct
      841 tgatccggtc ctgcaccgcc atgaccgtcg tcgtggcgac gagcgccagg accagtccca
      901 cgcaggcgta gcccagccag tgcgcaaaac caatcagctc ggcgaggtcc gaaagcgtgt
      961 cgcgctgaaa gacccccttc gagcgagttg acgtggccac ctgaccggtc ttgaactggg
     1021 catcgatctg gccggcgatc tgatcggggt cagcgccagg cgccacatgc acttccaatt
     1081 gcgtcaccgt tccgagccca ctcttgccac gcgccagttg caggaagtcg aggtgcgtat
     1141 agatatagtt gtcttccgca gcaatcgtgg agcggaaggt gccaaccaca ctcaccgtca
     1201 cgtcgccgat cgtgaaccgc tgacctgtct tgatcccgcg gcgctgtgcg accgcctggc
     1261 cgacgatcgc ggcgtcggtc cgtgaatcga actcggtcca gctccccgaa agcagcgtca
     1321 agtcgcgtgc gccccgtagt ttctccggcg gcatgccgtt aaagacgatc acatcgaggc
     1381 tggcccggca gttgttcgtg aagaccttga tcgggacgac ttctgcgacc cccggcatct
     1441 tggcgaccgt gcggccatag tcctctggca gcttgctgct ggccgggcag aaccgattct
     1501 cctggaagac gatcagggtg cgcatcgctt cctgatcttc ggtcagccgg gccagccctt
     1561 gctggaccgc gccgacgaag cagaaaacga acagggccac ggccgagccg ctcacggtca
     1621 gcagcgtgcg gctgcggtgc tgccacaatc ccttgaacac gtagggtgcg aatttgaaca
     1681 tgcttaggcc ctccgctcaa tggcttccac gccgacgacg gacacttcgc ggcccttctc
     1741 gatcaacttg ccttgctcta agcgcaattg ccgcttggcc agcgcggcaa ccttcggatc
     1801 gtgagtaacc aggagcagcg tcatgccaag ctcggtattc aatcgctgca caaggtccag
     1861 aatctgttcg gtggtgacgg cgtcgaggtc gcctgtcggc tcgtcgccga caatcaacgt
     1921 cggattggca acgattgcac gggcgatgcc cacgcgctgc tcctggccgc cggagagttg
     1981 tcttgggtag tggttggatc ggtcggtcag gttgaccgcc tccagggcaa tctgcactcg
     2041 cttgctgcgc tccgcactgg acatgggcag cagcagaagc ggaagctcga cgttttcgta
     2101 tgccgtcagc acggggatca aattgtgcat ctgaaagatg tatcccacgt tcgccgcccg
     2161 ccaatcggcg agctggccgc gcgacaaccg tgtaatgtcg gtaccgttga cgacaatctc
     2221 gccggaagtc gggcggtcga tcccggccag cgcgttgagc agcgtgctct tgcctgagcc
     2281 gctcgccccc atcagcgaca caaagtcgcc gcgctcgact tccagatcga cctcctggag
     2341 cggcgtgatg acttcgtccc ctttgcggaa ctgcttggtg agccggcgga tttccacgag
     2401 tgccatgtgc ttctccgtta tctcgtcgca gccgtgcgat cggcggcgga tgactgagtg
     2461 ttagggggcc ggtgtcgggc tgggcgaatc cttaccaagg ctgcggtcct cgcccgtcac
     2521 gcgcacgcgc gtgccgtctg cgattccctc gcgaccagac acgatgagct tgtccagggg
     2581 tgagagtccc tgggcgactt ccacgagctg actcgttccg gcctgtccga gctgcacgac
     2641 gcgccggcgg gccacgctcg tcgccggatc ggtgacccag acgacggctt cgttctcggt
     2701 cttctcgacc agttcacgcg gcaccagcat ccgcagcggg tcgttttcgc tgtcggtgct
     2761 ccggccgggg atctgcggcg cgagaaaggt cacctgggcg atcatgtccg gcttgatcac
     2821 agccggcggt tcatcgatcg aaactttgac ctgcagagtg ttcttcccaa tgtcggcctg
     2881 cgacgtgatt gccacgacct tgccggcgag cggctgaggc gaagcggctg tttgaatttg
     2941 cacaggctgg cccatttgtg cctgtggcac atcctccagc ctgacatcag cccgtacttg
     3001 caactgagcc gggtcgtaca agctgaccac ggcgctggca tcacgctccg atgcggcgtt
     3061 catccccatc aggcgttgtc ccggcctgga atggagcgcc agtacacggc cagtgatcgg
     3121 agcacggatc gtcattcgct ccaggcgcag attggccgcc tcgaccacca actcggcctg
     3181 ctcgacacgg gttttggccg attccacttg ggccgtggcc tccgcgagag cccgcgtttc
     3241 ttcggttttc agcttccagc gcgtatgcag cgcgtcgcac ttccgttgcc aggctttttg
     3301 ttggatttcc agcgttgggc cgcgctcctt caactcctgg agggcagcgg tcgcgctgtc
     3361 gaactcactt tgagcaagct gaacgacacg agccgccacc gcgtcggcca ccgccttctt
     3421 tcgggtaagc tcttgctcgg caaaggtcag acgcgcttcg gcggccttga tcagaaacgg
     3481 caggttcttg atttcggtat tcagcctggc caattccgcc tctgcttccg cgtgcgctgc
     3541 ttccagttgc acgggctgat cgaacgtcgc ttgcgcggct tcgaggcccg cctccgccac
     3601 gggcagttca gaccttcgca accgcagcgc cacgttcgcc tcgcgcgcgg cgagtctggc
     3661 ttccgcgtcg atcagcttcg ctacaggttg gcctgcggtg acctcttgac cttcgatgac
     3721 gagcaactta tccacgaccc cttcgacgag tgccgaagcc atcacggccg ttggccgcgg
     3781 ctcgatccag ccggctgcct ggaacagcgg cgtccctgcc tgctggactt cggctttggc
     3841 aaggatgacc ggcgtgaccg tgacctcctt ggcgggcagg aaccaatcgc gggccgacca
     3901 gccagtcaag accaagaatc cgcccgcgat tgccgacgga attccccacc gcgtcagcca
     3961 ggcccgcttc ttgaccagcg tgtgcgacgg agcgacgggc cgctcgactg cgagctgccg
     4021 gagatcgacg ggagtggaca tggaagtgct catggatgat cggggtcagt tgcgaacaaa
     4081 cacgccgtct gccagcacgg tcaagttgcc ggcgtcgtcg cgcttggcct tgccatgcac
     4141 cacgacggtt tgcagctcct tcagtccgag gagcttgcgg gcatcggtcg agacggtctt
     4201 gccgctgcca tcgaccacct tcaccatcgc cttgtgctcg ggcagcgagt cggcatcgca
     4261 gcaataatcc caaggtgtcg ggcaggcgtc gtcgtccttc tcgttgcagg gcttcaaggc
     4321 gcgatcgacg atcagaaagg ctgcctggcc ctcgatccag ggattcacgt cgccgccaac
     4381 gcggcccacc agcgtgatct cctcctcatc cttgatgact tccttggccg ctttgacgtc
     4441 tttggcgtct gcgggttcta cgtcgagtcg ataggcgctg ccctcggccg aagccgaatt
     4501 gttggcggga gcagtggcgc ttgcgccgca gccggtcaat ggaatgatgg cggcgagtaa
     4561 agccgacaaa atcgtgaggt ggtatttgga catgacgaac tccgggggtt agggaatcaa
     4621 acggctttca ggccctcaac gacggaagct cgcatggctt tgattgcggg tgggatcgcc
     4681 ccgacaaagc ccagcaggaa tccggtgccg cagccgacga ggatcgctac gccatccacg
     4741 cggagcgcaa acgctcccat cgtgaaccgc acggcagttc cgttgaccag aacgatggcc
     4801 acggcggccg cgagcaagga ggctgccatc gacaagagcg tggcctcctg gatcaggcta
     4861 atgactatgg cgcggcggcg gaatccaacc gcctgcaagg tggatagttc gcgcacgcgg
     4921 ccgacgaccg cgccgtacat ggtgttgagt ccggcgaaaa tccctgctcc ggccaccagc
     4981 aacacgacca gccaacccag catgcggaca ggttgatagt gcttctgcag cgagacgtag
     5041 tacgacgtct cgctgagcgc ctgtagttcg agatcaaagc gctctttgca gaatagctcg
     5101 atatcggagg gcgaagcgcc attggccagg ccgaccgcca cgaggctaac gtcctgacgc
     5161 ttaagggccg actgcaaatc ggccaaggca gcccagatct ctgactcaaa ggccgccccg
     5221 cccgcggcaa agcgcccgca gattcgccac tcctgcccgt caaaccggac cgtgttgccg
     5281 atttgcagtt tttcgggatc gcagccgagt ttgctggcgg cgaggcggcc gacgaggact
     5341 tcgttcgtgc ctggccagtt gccctccagc aatcgaacct gccgtcgcaa gagcggtgcg
     5401 gtgggcgtca cgccgcggac aatgccaaac attccaggac tgtttggatc ggtcagcacc
     5461 ttggttccga cgtacaactc gggagacgta taggtcaggc cgaagcgccg gtcgatcccg
     5521 tccaggctgg cggagagtag tgacgcggtg cccgccggaa tcgccgagtt ctcaatgtcc
     5581 gcggcggccc caatggaata gacgagcaca acgttcggtt cgccgctgac ggccagcgag
     5641 gtctccaggc cccggataaa cgccacgaca accagaacca gcaagacaac cgtcgatagt
     5701 gccgcgagcg tgagcgcgct gcgcgccggt cggcggaaca ggttgcggat gccgtattcc
     5761 cacggaagta gtcgagttct tagcactgca gttctcggga atctcaaaca gtttacggat
     5821 atcaatcgag cgaccaggca cagcaatact tcctcgcgcg cgtcatcgag aaatgcagaa
     5881 acgcacttcc aggtcgcgag gcgcagcgca agggcaaagc gtttagagca agaatgactg
     5941 gaccgcgatc cgcacctgcc gaccgtctcg cagaggaggc ggcggacctg ccggtcgcgt
     6001 cagccaatcg gcactcgtca attccagctc aatggccgaa tcgacggctg gtgcccagag
     6061 agaagcatcg agaagtggcg cggcggggcc gcgagcggtg gcatcaaag
//
LOCUS       DASUGB010000658         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_491723, whole genome shotgun sequence.
ACCESSION   DASUGB010000658 DASUGB010000000
VERSION     DASUGB010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491723"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFV87_12610"
     CDS             <1..583
                     /locus_tag="VFV87_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5104651.1"
                     /translation="ALVGVNLLREGLDLPEVSLVAILDADKEGFLRSETSLIQTIGRA
                     ARNVNAKVILYADKVTEAMRNAIDETSRRRAMQERYNAEHGITPETVKKSIRAGIEQA
                     IAAHRQANAAIGRGDDSVYITEEYVGELEAEMYKAAEELDFERAAALRDRIGQLRDSV
                     GQKLSEVEVKSYKPGPKRGRGGKGRVPRPKKNA"
     gene            complement(746..964)
                     /locus_tag="VFV87_12615"
     CDS             complement(746..964)
                     /locus_tag="VFV87_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104652.1"
                     /translation="MKKLFALVATCGLALTTIGCGETTTTTPTTSTPTPSTATPPADT
                     SKELTPPADTPAETTPPADTPAEKPATP"
     gene            1188..>2396
                     /locus_tag="VFV87_12620"
     CDS             1188..>2396
                     /locus_tag="VFV87_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5104653.1"
                     /translation="MSHPTSGQPNDTAVIPENPHALDVGEIAQLLKSDVEQGLSDTQA
                     AERLQEFGLNQLAEAPRTPLLRRLARQFQQLVIWILVVAAVIAGVMGDLVDTVAILAI
                     VLLNGLIGFFQEERAEQALAALRKMSSPQTKVTRGGRTRSVATTEIVPGDRVEIEAGD
                     NIPADARLVEAFSLRVQEASLTGESTPVEKDPKAKLAADASLGDRENMLYMGTVAAAG
                     KASAIVVGTGMSTELGHIAGLLQRQEAEQTPLQRRLEELGRVLVYICLSLVALVFVLQ
                     MMRGGKLLEVLLVSVSLAVAAVPEGLPAVVTLALALGLQRMARRHALVRKLPSVETLG
                     SVTVICTDKTGTLTRNEMTVRALITPSGEYEVTGTGYAPQGEFRAGDQTVHKPQDRPD
                     LRLLLTAGAWC"
BASE COUNT          462 a          704 c          832 g          398 t
ORIGIN      
        1 ggccctggtg ggcgtaaatt tgctgcggga agggctcgac ctgccggagg tctcgctcgt
       61 ggcgattctc gacgccgaca aggagggttt cctccgtagc gagacgtcgc tgatccagac
      121 gatcggccgg gcggcccgca acgtgaatgc caaggtgatc ctctatgccg acaaggtgac
      181 cgaggcgatg cgcaatgcca tcgacgagac cagccgccgt cgggccatgc aggagcgata
      241 caacgcggag cacggcatta cgcccgagac ggtgaagaag agcatccggg ccgggatcga
      301 gcaggcgatc gccgcgcacc ggcaggccaa tgccgccatc ggccggggcg acgattcggt
      361 ctatatcacc gaggaatacg tcggcgaact cgaagcggaa atgtacaaag cggctgaaga
      421 gttggacttt gagcgggcgg cggcgctgcg cgaccggatc ggccaactgc gcgattccgt
      481 gggccaaaag ctgagcgagg tcgaggtcaa gtcgtacaag cccgggccaa aacgtggccg
      541 cggcggtaaa gggcgcgtgc ctcggccgaa gaagaatgcc tgaggagcgg gtggtccgtc
      601 gtcagtggtc agaagacaga cgacagagga gatcgcggtg tcgcgagtca ttccgccttt
      661 gcaagaaaag acccagggag acgagccggc tggctgtctc cctggcttga acgagtcgtt
      721 ctgcttatcg gcgcctgacg ccaggttagg gcgtggcggg cttttcggcc ggcgtgtctg
      781 ccggaggagt ggtctcggcc ggcgtgtcag caggaggagt caactccttc gaagtatcgg
      841 cgggaggcgt cgccgtggac ggcgtggggg tgctcgtcgt tggcgtggtg gtcgtcgttt
      901 caccgcagcc gatcgtcgtc agcgccaggc cgcacgtagc caccagggca aacagcttct
      961 tcatggcttg gttttcctat tcgggtaagc cagacagaga ttggacagcg ggacattccg
     1021 gctgtctcag ggtctcgcag ttgagagccg ttttgttgcg gacttattcc ctgccggcgg
     1081 gatttttgca gaataagcgt gcggcatcac tgcggcagcc atccgcctga acgattcgga
     1141 tgttcgctaa cccgcaagcg tgtaaaggtt tacagcaaga caatccagtg tcccacccaa
     1201 cgagcggcca gccgaacgac accgcagtca ttcccgaaaa cccacatgcg ctggacgtgg
     1261 gtgaaattgc ccagcttctc aagtcggatg tcgaacaagg gctgagcgac acgcaggcgg
     1321 ccgagcgtct tcaggaattc ggcttgaacc aactggcaga agcgccccgc acgccgctgt
     1381 tgcggcgact ggcgcggcaa ttccagcagc tggtgatctg gatcctggtg gtggcggcgg
     1441 tgattgccgg agtgatgggg gacctggtcg ataccgtggc cattctggcc atcgtcctgc
     1501 tcaacgggtt gatcggcttt tttcaggaag agcgggccga acaggccctg gcggccctgc
     1561 ggaaaatgtc ctccccccaa acgaaagtga cgaggggcgg gagaacgaga tccgtcgcca
     1621 cgaccgaaat cgtgcccggc gaccgcgtgg aaattgaggc cggcgacaac atccccgccg
     1681 acgcccgcct cgtggaagcc ttcagcctgc gggtgcagga agcctcactg acaggcgaat
     1741 cgacccccgt cgaaaaggac cccaaggcga agctcgctgc ggatgcctcg ctgggggacc
     1801 gcgaaaacat gctctatatg ggcacggttg ccgcggcagg caaggcgagc gccatcgttg
     1861 ttggaaccgg gatgagcacc gaactggggc acatcgccgg gctgctccag cggcaagagg
     1921 ccgaacaaac cccgctgcag cggcgactgg aagaactagg gcgcgtgctc gtgtatatct
     1981 gcctgagtct ggtggccctg gtgttcgtcc tgcaaatgat gcgcggcggc aagctgctcg
     2041 aagtgctgct ggtttcggtg agcctggccg tcgccgccgt gccggaaggg ctgccggcgg
     2101 tggtcacgtt ggccctggcc ctggggctgc agcggatggc caggcggcac gccttggtgc
     2161 ggaagctgcc gagcgtcgag acgctgggat cggtcacggt gatttgcacc gataagacgg
     2221 ggacgctaac gcgcaacgag atgacggtgc gcgccctgat cacccccagc ggcgaatacg
     2281 aagtgaccgg caccggctat gccccgcagg gcgaattccg cgccggcgac caaacggtgc
     2341 acaagccgca agaccggcca gatttgcggc tgctgctcac cgccggcgcc tggtgc
//
LOCUS       DASUGB010000659         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_768713, whole genome shotgun sequence.
ACCESSION   DASUGB010000659 DASUGB010000000
VERSION     DASUGB010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768713"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /locus_tag="VFV87_12625"
     CDS             complement(<1..2241)
                     /locus_tag="VFV87_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104654.1"
                     /translation="MAPLLPDNGDTRTFNGNMLNEAIATPGSIQQATVNISGALKKAV
                     NLTPFSFDPTFNGMWDEFTTVDRKTVDDQGLPAAHNADDLIFGGLGSDWLHGGSGDDA
                     ILGGEALGEAYTQRYDGITLVGVARSDYNRPFNPVDALRYNPIDVDGWHRDSTRRAGE
                     FALYDEYDPLRKITLNVDGTANKSDAGGLFPWFLNFSTAEGVYVPAGTIPGTGQQATI
                     YAETWNDGDDRIFGDTGNDWLVGGTGRDDVYGGFGNDLLNADDDHATNLLRNDQPDTQ
                     PSFEDRAFGGAGRDVLIGNTGGDRLIDWVGEFNSYLVPFAPFGMATVSRTLQPQLAEF
                     LYTLSASDGADPTRWADTNADPALRYRNGEPEGELGVIRQKDFAWQSQTGAPADPQAG
                     NIPGGQRDVLRTANFNDGTTQALAPDSGIWLVSGGALQVAAASQKDDAVAVYQIGDAL
                     PSYFEVLAKIKVIKPTGGWNANSYVIFDYQSPTNFKYAGLNVSTNKLVMGQRTTAGWQ
                     DLRQASFPGSIKNETWYNVMLSVNGLTATLIVNNTNVFTHTFSASVVDGWSYGLNWGL
                     VGFGSNKSRGAMDNITVQVLPPQVTLDVTEDFHDGVADLFTGDQTGLWTATDGRYSST
                     TSAGQTHIDTLDFGIGHFQTTSYLELQSTFQVSTANGIGGLVFDEYAPNDFKFVALDI
                     ASQKVLVGHFEPRRGWVVDASIAKLLTANTDYAVQVTLKGSSVSVTVGGALTVSHAFH
                     APIMDDA"
BASE COUNT          385 a          695 c          757 g          465 t
ORIGIN      
        1 cgcatcatcc atgatggggg cgtggaaggc gtggctcacg gttaaggcgc ctccgaccgt
       61 gacgctgacc gaacttccct tcagagtgac ctgcacggca tagtcggtgt tcgcggtgag
      121 caatttggca atcgaggcat cgacgaccca cccgcggcgc ggctcgaagt ggcccacgag
      181 cactttttgc gacgcaatat cgagggctac aaacttgaag tcgttaggcg cgtactcgtc
      241 gaagacaagg ccgccgattc cattcgccgt tgacacctgg aacgtggact ggagttccag
      301 ataggaggtc gtttggaagt ggccaatgcc gaaatcgagc gtatcgatgt gggtttggcc
      361 ggctgacgta gtgctcgagt agcggccatc tgtggcggtc cacagtccgg tttgatcgcc
      421 ggtaaagaga tcggccacgc cgtcatggaa atcctcggtc acatccagcg tgacctgggg
      481 cggcaacacc tgcacggtga tgttgtccat cgcccctcgc gatttgttcg agccgaagcc
      541 gacaaggccc cagttcagcc cgtagctcca gccatcaacc accgaggcgc taaaagtgtg
      601 agtgaagacg tttgtgttgt tgacaatcag ggtcgccgtt aagccgttga ccgacagcat
      661 cacgttgtac caggtttcgt tcttgatgct gccggggaag gaagcctgcc gcagatcctg
      721 ccagccggcc gtggtgcgct ggcccatgac cagcttgttg gttgagacgt tcagtccggc
      781 gtacttgaag ttggtcgggc tctggtagtc gaagatcacg taactattgg cgttccaacc
      841 gcctgtgggc ttgatgacct tgatcttggc cagcacctcg aagtaggagg ggagtgcgtc
      901 gccgatctgg tacaccgcaa ccgcgtcatc tttttgggat gcggctgcca cttgcagtgc
      961 gccgccgctg accagccaga tgccgctgtc cggcgccagc gcctgcgtcg tgccatcgtt
     1021 gaagttggcc gtgcgcagca cgtcgcgctg accgccgggg atattgcccg cctgcggatc
     1081 cgccggggca ccggtctggc tttgccacgc gaagtccttt tgccggatca cgccgagctc
     1141 gccctcgggc tcgccgttgc gatagcgcaa ggcgggatcg gcgttggtgt cggcccaacg
     1201 cgtcggatcg gctccgtcgc tggcgctgag cgtgtagagg aactcggcga gctgcggttg
     1261 caaggtgcgg ctcacggtgg ccatgccgaa cggggcgaaa ggcaccaggt agctgttgaa
     1321 ctcgcccacc cagtcgatca ggcgatcgcc gccggtgttg ccgatcaata cgtcgcggcc
     1381 ggcgccgccg aaggcacggt cctcgaagct cggctgcgta tcgggctggt cgttgcgcag
     1441 caagttggtg gcgtggtcat cgtcggcgtt caagaggtcg ttgccgaagc ccccgtagac
     1501 gtcgtcgcgg ccggtgccgc cgaccagcca gtcattaccg gtgtcgccga agatgcggtc
     1561 gtcgccgtcg ttccaggttt cagcgtagat tgtggcctgc tgccccgtcc cggggatcgt
     1621 tcccgccggc acatagaccc cttcggcagt tgagaagttc aggaaccatg ggaagaggcc
     1681 gccagcatcc gacttatttg ccgtcccatc gacattgagc gtgatttttc gcaacgggtc
     1741 gtattcgtca tagagcgcga attcgccggc gcggcgggtg ctgtcgcggt gccagccgtc
     1801 gacatcgatc gggttgtagc gcagcgcgtc cacgggattg aacggcctgt tgtagtcgct
     1861 tcgcgcgacg ccgacgagcg taatgccgtc gtagcgctgg gtgtacgctt cgccgagcgc
     1921 ctcaccgccc agaatggcat cgtctcccga gccgccgtgc agccagtcac tgcccaggcc
     1981 gccaaaaatg aggtcgtcgg cgttgtgggc cgccggcagc ccctggtcgt ccaccgtttt
     2041 gcggtccacc gtggtgaact cgtcccacat gccgttgaac gtcggatcaa agctgaacgg
     2101 agtcagattg accgccttct tcagcgcgcc ggagatgttg acggtcgcct gctggatcga
     2161 gcccggcgtg gcgatggcct cgttgagcat attgccattg aacgtccttg tatcgccatt
     2221 gtcgggcaag agcggggcca cgccgtggag ggcttcgccc aggctcgaga ggtagcccgg
     2281 gctgccgggc acgctgcttt cg
//
LOCUS       DASUGB010000660         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_768837, whole genome shotgun sequence.
ACCESSION   DASUGB010000660 DASUGB010000000
VERSION     DASUGB010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768837"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..>2074)
                     /locus_tag="VFV87_12630"
     CDS             complement(102..>2074)
                     /locus_tag="VFV87_12630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5104655.1"
                     /translation="KEAARQRPITIALLATLLRQANEPTKASELLRRARAQHPAEFCL
                     NLALGIACLPGDSWESERYLHLCAIERPTNASVRTALGLVLFENEYVDDAENELREAI
                     RLAPSYAEPHLQLAGLLQAAQQPMEAEAEFRQAMRLNPWLYAPPEKLLALLAEQNKLA
                     EADRALHEAVDRTPEAAPAYLCLADLLARQSKWSEAHAVYQQAVRLDHRAAKRARSLT
                     LAFLGQDGAGEAARHLMTVLEADPDDHLLAMQTAILQLQLGNHQAHADVCRRLLARYR
                     DTKDPLEADRTAKACLCSIPPVGELEELTRLAQVAVTEGKSHSFYCYFPLVRALAAYR
                     AGDWPAALQWSAEGLHLVDSAPNLRTLKAHYLVVKAMAQHRSGDPGAAIVTLESAQSI
                     INILYPEAPKKLGVMWADWSLYDILRKEAVQFIPVLSSDGGLNEKFLAATRGDWDLAA
                     QGYSAAVRSDPEDDGSALRAAVLHLYLHHQSEYQALARQMLERSGPMSTAITAHRTSF
                     ACLLASPPVGAMPQLEELAELASNTGGETRQAVLLYGRGRGLAAYRAGRWEEAIKWCN
                     QSRGDGQGLTQSINVPMQDMLIEAMALHHLGKTADAQQRFDQAEALIQEQFPGPSTSI
                     GKAWFDWLVYQILRREAEPLLKGGDAKPPDSR"
BASE COUNT          347 a          659 c          703 g          365 t
ORIGIN      
        1 ccgccaatgc cgatgcacag ggcgaggatt gccgttcatg gagcgctccg tggtgaaaca
       61 ttgccagaca acgacgcggc cacaacatcg cggcgcggcc gctaccgaga atccgggggc
      121 ttggcatccc cgcccttgag gagcggttct gcttcccgac gaagaatctg atacaccagc
      181 cagtcgaacc acgctttgcc gatgctggta gaagggccag gaaactgctc ctggatgagc
      241 gcttcggcct ggtcaaaccg ctgctgcgcg tcggccgtct ttcccaagtg gtgcagggcc
      301 atagcctcga tcagcatgtc ctgcatcggc acgttgatcg actgcgtgag tccctggccg
      361 tcgccgcggc tctggttgca ccacttgatc gcctcctccc agcgccccgc gcgataggcc
      421 gccagcccgc ggccgcggcc gtagagcagg accgcttgcc gggtctcacc cccggtgttg
      481 gacgccaatt cggccaattc ctcgagctgc ggcattgcgc cgacaggcgg ggatgccagc
      541 aggcacgcaa acgatgtccg atgggccgtg atcgcggtgg acatcgggcc gctgcgctcg
      601 agcatttggc gggccagcgc ctggtactcc gattgatggt gcaaatacag gtggagcacc
      661 gcggcccgca acgcggagcc gtcgtcttcc gggtcgctgc gcacagctgc cgaatagccc
      721 tgggccgcaa ggtcccagtc tccgcgggtg gcggccaaaa atttttcgtt gaggcccccg
      781 tcggaggaca ggaccggaat gaactgcacc gcttcttttc gcaggatgtc gtagagcgac
      841 caatcggccc acatgacgcc gagtttcttg ggagcttccg ggtacaggat gttgatgatc
      901 gactgggctg attcgagcgt cacgatcgcc gcgccgggat cgcccgaccg gtgctgcgcc
      961 atggccttga cgaccaaata gtgggccttg agcgtgcgca ggttcggcgc cgaatcgacg
     1021 aggtgcagcc cctcggcgct ccattgtaag gccgccggcc aatccccggc gcggtaagcc
     1081 gcgagcgctc gtaccagggg aaaatagcag tagaagctgt gcgacttgcc ctcggtgacg
     1141 gcaacctgtg ccagccgggt cagctcttcc aactcgccga ccggggggat ggaacagagg
     1201 caggccttgg cggtgcggtc ggcttcgagc gggtctttcg tgtcgcgata gcgcgccagc
     1261 aagcggcggc aaacgtcggc atgggcctgg tggttgccca gctgcagttg caggatggca
     1321 gtctgcatgg cgagcagatg atcgtcggga tcggcctcca aaacggtcat caggtggcgg
     1381 gccgcttcgc ccgcgccgtc ttggcccagg aacgccagcg tcagactgcg ggctcgcttg
     1441 gcggcccggt gatcgagacg aacggcttgc tggtaaacgg cgtgggcttc gctccacttg
     1501 ctttgccggg cgagcaagtc ggccaggcag agataagccg gggccgcctc gggagtgcgg
     1561 tccacggctt catgcaaagc gcgatccgcc tcggccaatt tgttctgctc ggccagcagc
     1621 gccaggagct tctccggcgg cgcatagagc cacggattga gacgcatggc ctggcggaat
     1681 tccgcttccg cttccatggg ctgctgcgcg gcctgcagca atccggccaa ttggaggtgg
     1741 ggctcggcat agctgggcgc caagcgaatg gcttcgcgga gctcgttctc ggcgtcgtcc
     1801 acgtattcgt tttcgaacag caccaggccc agcgccgtgc ggacgctggc gttggtcggc
     1861 cgttcgatcg cgcacaggtg caaatagcgt tcagactccc acgaatcgcc gggaagacag
     1921 gcgattccca gcgccaaatt gaggcaaaac tcggcggggt gctgggcgcg ggcccggcgg
     1981 agcagctcgc tcgctttggt cggctcattg gcctgacgca acagcgtcgc caagagcgcg
     2041 atggtgatcg gccgctgccg cgccgcttcc ttgg
//
LOCUS       DASUGB010000661         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_352635, whole genome shotgun sequence.
ACCESSION   DASUGB010000661 DASUGB010000000
VERSION     DASUGB010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352635"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFV87_12635"
     CDS             complement(<1..202)
                     /locus_tag="VFV87_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104656.1"
                     /translation="MASKAFAAPGSAAAAILAVTATAIAQQPPYDDYPPADPPYYRVR
                     YEASQEPGELAYAVNYTIWIPPG"
     gene            complement(225..1358)
                     /locus_tag="VFV87_12640"
     CDS             complement(225..1358)
                     /locus_tag="VFV87_12640"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5104657.1"
                     /translation="MRTSTNLIASPPASSCDEFAPAGGCGRRSFLAGIAGAAAAFCSS
                     QAMGRDYGPGAQPVRYPDPDIVVLDKRFEAYKLGNTPIQRLYHSPNMLWAEGPAWSAV
                     GKYLLWSDIPNNVQMRWLEEDGHVSVFRNPAGNSNGNTFDYQGRQIACEHGNRRVVRY
                     EHNGKTTVLADNWQGKKLNAPNDAVVHPDDGAIWFTDPGYGSLMNYEGVKGKLEIKEA
                     VYRIDAQSGKMDLVTDEIFKPNGLCFSPDYKKLYVADTGASHYPDAPRNIKVWDVVSG
                     KALANGREFVSMQLDGFDKAGFADGIRADIDGNVWASAGWVGDGYDGVHIFAPDGDRI
                     GLIRLPEICSNVCFGGAKRNRLFMTGSTSLYAVYVETQGAHIT"
     gene            complement(1440..>2527)
                     /locus_tag="VFV87_12645"
     CDS             complement(1440..>2527)
                     /locus_tag="VFV87_12645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5104658.1"
                     /translation="QAADVDSLTDYFPPTEDQGGWRTLLPETGEPSADQKAEIRKVTG
                     CDWDKLLGAWQHNAAAPGATGLIVIRKGYVVGEWYRDGDRTKAFNIYSSSKAYTSTAL
                     GLILNDFGNGELPSGKSLTLDTKVCNADWIPESLPLSDPRKSDITVRHLLNMISGVGG
                     EPVPAKAPFETSLGHTNDSPFAKLKGAPGTVFNYSNAGVSHLVLLFNHAQGMDLFPFL
                     KERLFDRIGMQRVEWMKIGGDGNIGPYNQGYSGVLTNPREHARFCYLALHKGKWADKQ
                     IVPAAYYDFAWTPSEVKNNYGGQWWTFPELPGGPADLVITRGKDFNDGWVVPSLDLVF
                     VRLGDGQQFPKDFEKDLVLKVLAAVEK"
BASE COUNT          452 a          777 c          788 g          510 t
ORIGIN      
        1 cgccaggggg gatccagatt gtgtagttga ccgcgtaggc cagctcgccc ggctcctgcg
       61 atgcctcgta ccgcacgcga taatagggcg gatccgcggg tggataatcg tcgtacggtg
      121 gctgctgggc aatcgccgta gcggtcacgg cgaggatcgc tgcggccgca ctgcccggcg
      181 cggcaaacgc tttcgaagcc atgctcagag tcctggtgcc agaatcaggt gatgtgtgcg
      241 ccctgcgtct ccacatagac cgcatagagc gacgtgctgc cggtcatgaa cagccggttc
      301 cgcttggcgc cgccgaagca gacgttgctg cagatctccg gcagccggat gagcccgatg
      361 cgatcgccgt cgggggcaaa gatgtggacg ccgtcgtaac cgtcgccgac ccagccggcg
      421 ctggcccaga cgttgccgtc gatatcggcc cgaatgccgt cggcaaagcc cgctttgtcg
      481 aagccgtcga gctgcatcga aacgaactcg cggccgttgg cgagcgcctt cccgcttacc
      541 acgtcccaga ccttgatgtt ccgcggcgca tcgggatagt gtgaggcccc cgtgtcggcg
      601 acatacagct ttttgtaatc gggcgaaaag cagagcccgt tcggcttgaa gatttcgtcg
      661 gtcaccaggt ccatcttgcc gctctgggcg tcgatgcgat acacggcttc tttaatctcc
      721 agcttcccct tgacgccttc atagttcatc aagctgccgt agcccgggtc ggtgaaccag
      781 attgcgccgt cgtccggatg gacgaccgcg tcgttggggg cgttcagctt cttgccttgc
      841 caattgtcgg cgagcacagt ggtcttgccg ttgtgttcgt agcgcacgac gcgacgattg
      901 ccgtgctcgc acgcaatctg ccgcccttga tagtcaaacg tgttgccgtt gctgttgcca
      961 gccggattgc ggaacacgct cacgtggccg tcttcctcca gccaccgcat ttgcacgttg
     1021 ttggggatgt cgctccagag cagatacttg ccgacggcgc tccaggccgg cccctcggcc
     1081 cacagcatat tcgggctgtg atacagccgc tggatcggcg tgttgcccag cttgtaagct
     1141 tcaaaccgct tgtccaggac gacgatgtcc gggtcgggat agcggaccgg ctgggcgcca
     1201 ggtccataat cgcgtcccat tgcctgagac gagcaaaacg cggcagcggc tccggcaatg
     1261 ccggccagga atgagcgccg gccgcaacct cctgcgggag cgaattcgtc acaagaggac
     1321 gccggcggag aggcaatcag gttggtgctg gtgcgcatag gaactcccgt tgcaaggcat
     1381 gaggcagata tcgagcgtat tgttatcgag cgtattgtac tttggcggat ctcggagtgt
     1441 tacttttcga ccgctgccag caccttcagg acgaggtcct tctcgaagtc cttgggaaat
     1501 tgctggccgt cacccaggcg gacgaaaacc agatcgagac ttggcacgac ccagccgtcg
     1561 ttgaagtcct tgccgcgcgt gatgaccaga tcggcgggcc cgccgggcag ttccgggaag
     1621 gtccaccact ggccgccata gttgtttttg acctcggacg gcgtccaagc gaagtcataa
     1681 taggccgcgg gaacgatctg cttgtcggcc cacttgccct tgtgcagcgc caggtagcaa
     1741 aagcgagcgt gctcgcgcgg attggtcagg actccgctgt agccttggtt gtacgggccg
     1801 atattcccgt cgccgccgat cttcatccat tccacgcgct gcatgccgat ccgatcgaac
     1861 agccgctcct tgagaaacgg gaataaatcc atgccctgcg cgtggttgaa cagcaggacc
     1921 aggtgcgata cgccggcatt gctgtagttg aacacggtgc ccggagcgcc cttgagcttg
     1981 gcgaacggcg aatcattggt gtggcccagt gaggtctcga acggcgcttt ggccggcacc
     2041 ggctcgccgc cgactcctga aatcatgttg agcaagtgac ggacggtgat gtcggatttt
     2101 cgcgggtcag acagcggaag cgattcggga atccagtcgg cgttgcaaac cttcgtatcc
     2161 aacgtgagcg acttcccgct gggcagctcc ccattgccaa agtcgttaag aatcaagccc
     2221 agcgcggtgc tggtgtaggc tttgctgctg gaatagatgt tgaaggcctt cgtgcggtca
     2281 ccgtcgcgat accactcacc cacgacgtag cccttgcgaa tcacaatcag gcccgtcgct
     2341 cccggagccg cggcgttgtg ctgccaggcg cccagcagct tgtcccaatc gcaaccagtg
     2401 accttccgaa tctcggcctt ttgatccgcg ctgggctcgc ctgtttcggg cagcaaagtc
     2461 cgccaaccgc cctggtcctc ggtgggcggg aaatagtcgg tcagcgaatc gacgtcggcc
     2521 gcctggc
//
LOCUS       DASUGB010000662         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_769174, whole genome shotgun sequence.
ACCESSION   DASUGB010000662 DASUGB010000000
VERSION     DASUGB010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769174"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1403)
                     /locus_tag="VFV87_12650"
     CDS             complement(165..1403)
                     /locus_tag="VFV87_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX5104659.1"
                     /translation="MLEILIKSGILIGGLMTAAAYFVLLERRMAAWTQDRIGPSRVGA
                     PLSLFGFKDLRLWGLGQPAADGLKMMLKEDYTPAHVDRKLYILAPLAILAAAIATSAV
                     IPFGSVLPPMTIGGEKVGPIHLVVAPGVDVGMIYVFALSSVAVYGVILGGWASNNKYS
                     FIGGLRSSAQLIAYELPMGLGILGVVLAAGSLDLKKIMGFQANYGWFALAQPLGFVVF
                     LVAAFAEAARLPFDLPEAEQELIGGYHTEYAGMKLLLFLVAEFLHMIVASFLIVILFF
                     GGWHLPIVTGSDPDSATWFQAIARIVVLHAKIFAVIIFFMWVRWSWPRFRFDQLMNLA
                     WKIMLPLGLANFVAIAILYEFRKLYDPAERSLAWTLGIVAASWLVFGLAWIAVSLSNP
                     LVTDNRPRGNLSPAGIDPQI"
     gene            complement(1527..3161)
                     /locus_tag="VFV87_12655"
     CDS             complement(1527..3161)
                     /locus_tag="VFV87_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5104660.1"
                     /translation="MPSVLIDNIEVSLGDKERLNGIQAARRAGVEIPHYCWHAGLSVV
                     ASCRMCLVECGKKDDKTGKIAMQPRLVPACQTPATDGTVFVTNSDKVKQARAMVEEDL
                     LLRHPIDCPICDKAGECSLQDYHFEYGQSQRRADIQPFTSRRRELGDTVTLFVDRCVM
                     CSRCVRFTREITGTSELMVINRGAHEEIDVFRDEQGNIQFPLANKMSGNVCDICPVGA
                     LGDKDFLYEQRVWFLKSHANVCAGCSTGCSIHVEENQDRVYRLKPRENPHVNQWWMCD
                     EGRYGWKHVHDHHRVTQLRRKEKDGLHNIEWSHLPQELDQKLKKAGRLAAVISPHLTV
                     EEAYLLCTYVRSIDQQAVLAVGPIPTEGTDETFPSGFTIRAEKCPNRKGVEAIVGKLG
                     GGLANWGDFLGQVQNETFGAVWVAGGYRQPWHDEAVAEKFAGIKLLIVQDCFSSPLWN
                     QADYQLPGGTFAEREGSYVNASDRLQSFKWAIRPPAGAKVEGQLYWQLLNKPGLYRAS
                     EVLAEIAREILYFSAAMKGVPDVGIDLKVNQLANTS"
BASE COUNT          585 a          948 c         1107 g          617 t
ORIGIN      
        1 ggctcctcga tccacttgat gttggggtcg tcgggtttca tgcttcagtc acgttgccga
       61 gtgcggcggt gtgcgctata gggaacggcc actgtgccgt tccggaggtg gcgggcttgc
      121 gagcggttca cggaacgcca cggcgggcgt tccctacagg attcctatat ctgcgggtcg
      181 attcccgcgg gcgacaggtt gccgcggggg cggttgtcgg tcacaagcgg gttggaaagg
      241 gatacggcga tccaggccag gccgaagacc agccaggagg cggcgacgat gccgagggtc
      301 caggccagac tccgctcggc cgggtcgtag agcttgcgga attcgtacag gatggcgatg
      361 gcgacgaaat tcgccagccc cagcggcagc atgatcttcc aggccaggtt catgagctgg
      421 tcgaagcgga accgcggcca gctccagcgg acccacatga agaaaataat gactgcgaag
      481 atcttggcgt ggaggacgac gatgcgggcg attgcctgga accacgttgc cgaatcggga
      541 tctgagcccg tgacgatcgg cagatgccag ccgccgaaga acaggatcac gatcaggaat
      601 gaggccacga tcatgtgcag gaactcggcg accagaaaca gcaagagctt catgccggca
      661 tattcggtat gatagccgcc gatcagctcc tgctcggctt cggggaggtc gaacggcagg
      721 cgggccgctt ccgcgaaggc ggcgaccagg aacacgacga acccgagcgg ctgggcgagg
      781 gcgaaccagc cgtaattggc ctggaagccc atgatctttt tcagatcgag cgagccggcg
      841 gcgaggacga cgccgaggat acccaggccc atcggcagct cgtaggcgat aagctgggca
      901 ctggagcgga gaccgccgat gaagctgtat ttgttgttgc tggcccagcc gccgaggatc
      961 acgccataga cggcgacgct gctgagagcg aagacgtaga tcatgcccac atcgacgccc
     1021 ggcgcgacga cgaggtgaat cgggccgact ttttcgccgc cgatcgtcat cggcggcagg
     1081 acgctgccga agggaatgac ggcgctggtg gcgatggccg cggcaagaat ggccagcggc
     1141 gcgaggatat acagcttgcg atcgacgtgg gccggcgtgt agtcttcctt gagcatcatc
     1201 ttcagcccgt cggccgccgg ctggccgagg ccccacagcc ggaggtcctt gaagccgaag
     1261 agcgagaggg gcgcgccgac gcggctgggg ccgatgcggt cttgggtcca ggccgccatc
     1321 cgccgttcca gcagcacgaa ataagctgcc gcggtcatca agccgccgat gaggattccg
     1381 gactttatga ggatttcgag catcttagcc cgtagcgcaa gcgagagtga tggaggtcgc
     1441 ccgcggcgtt gcttgtgagt cggggtgtag tggaattgtg gagcgtttgg gggttgaaaa
     1501 tcgattaccc tcgcttgcgc tacgggctac gatgtgttgg ccagttggtt gaccttaagg
     1561 tcgataccga catcggggac gcccttcatg gcggccgaga agtagaggat ttcgcgggcg
     1621 atttccgcga ggacttcgct ggcgcggtag aggccgggtt tgttcaggag ctgccaatag
     1681 agctggcctt cgaccttcgc gccggcaggt gggcggatgg cccacttgaa cgactgcagg
     1741 cggtcactcg cgttgacgta cgagccttcg cgctcggcga acgtgccgcc gggaagctga
     1801 tagtcggcct ggttccaaag cggcgaacta aagcaatctt gtacgattag cagcttgatg
     1861 ccggcgaatt tttcggcaac cgcttcgtcg tgccacggct gccggtagcc gccggcgacc
     1921 cagactgcgc cgaaggtctc attctggact tgtcccagaa aatcgcccca gttggctagc
     1981 ccgccgccga gcttgccgac gatcgcttcg acgcccttac ggttcgggca cttttcagcc
     2041 cggatcgtga agccgctggg gaacgtctcg tcggtgcctt cggtgggaat cggcccgacg
     2101 gcaagaaccg cctgctggtc gatgctgcgg acataagtgc agagcaggta cgcttcctca
     2161 accgtcagat gcggcgagat cacggcagcc aggcggccgg ctttcttcag cttctggtcg
     2221 agctcttgcg gcaggtgcga ccactcgatg ttgtggagcc catccttttc cttgcgtcga
     2281 agttgcgtga cgcgatggtg gtcatgcacg tgcttccagc cgtacctccc ttcgtcgcac
     2341 atccaccact ggttgacgtg cgggttctcg cggggcttga ggcgatagac gcggtcctgg
     2401 ttctcctcga cgtggatcga gcagccggtg gagcagcccg cgcagacgtt ggcgtggctc
     2461 ttgaggaacc agacgcgctg ctcgtagagg aagtccttgt cgccgagcgc accgacgggg
     2521 cagatgtcgc agacgttgcc cgacatttta ttggccagcg ggaactggat gttgccttgc
     2581 tcgtcgcgga agacgtcgat ctcttcatgg gccccgcggt tgatgaccat cagctcgctg
     2641 gtgccggtga tctcgcgcgt gaaacggaca cagcggctgc acatcacgca gcgatcgacg
     2701 aacagcgtga cggtgtcgcc cagctcgcgg cggcggctgg tgaagggctg gatatcggcc
     2761 cgccgctggc tttggccgta ttcgaaatgg tagtcttgca gcgagcactc gcccgctttg
     2821 tcgcagatcg ggcaatcgat cggatggcgg agcagcaggt cctcctcgac cattgcccgg
     2881 gcttgcttga ccttgtcgct gttggtgacg aagaccgtgc cgtcggtcgc cggcgtctga
     2941 caggcgggga ccaatcgcgg ctgcatcgcg atcttgcccg tcttgtcgtc cttcttgccg
     3001 cattcgacca ggcacatgcg gcagctcgcc acgaccgaca atcccgcgtg ccagcagtag
     3061 tgcggaatct ccacgcccgc ccgccgcgca gcctgaatgc cgttgaggcg ctccttatcg
     3121 ccgagggaga cttcgatgtt gtcgatgagg accgaaggca tgtttttagc cgggagcaaa
     3181 cttgctcccg gagaactgtg cattgaattt gctcgcggcg ctccgggacc aagtttggtc
     3241 ccggctagtg ggctagt
//
LOCUS       DASUGB010000663         4018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_492466, whole genome shotgun sequence.
ACCESSION   DASUGB010000663 DASUGB010000000
VERSION     DASUGB010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492466"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFV87_12660"
     CDS             <1..397
                     /locus_tag="VFV87_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104661.1"
                     /translation="TNALSGEAVIQTFEALRCNLGLIGVSGVAPSGGLFVKHRWEAKY
                     RAAIVRTVSNEIYICTDVEKLGNHDLWQFATIDEIVADKPKSRVVLITNKPRELREHR
                     LKVRAEETLRELTAISKRLGPQRFEVVVV"
     gene            complement(417..2048)
                     /locus_tag="VFV87_12665"
     CDS             complement(417..2048)
                     /locus_tag="VFV87_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5104662.1"
                     /translation="MRVTSVLSCSFFAWALSLPLSAAEPFSFAEVSAATGLKQHLEGD
                     EKTRPWRYAHGAAWGDVDNDGRPDLFLGAFAARAWFNGDDAPLPNRLFLNQDGKLNPA
                     SDDTLTARAADSRCAGAAFLDLDNDRDLDLVVTNHVTKDQGGSRVFENLGAGKFRDVT
                     PPGAPWNEVIGMRNVAGIDFNDDGLLDLVLTDGSYGRDAATTSRLRVLQNGGQFQFAD
                     MTADYGFPLDKTPGLGLALGDMNNDGRIDIFVAGSNRLFVSQAKGGKYREVENPALQY
                     PLLDAREGLRCGAALADLDGDDLLDLVVTEHGVPTRLHVFRTTAIKDGIPRLADVSDA
                     VGLGATFPSGTKEHPVKTAHVALRDLDNDSKIDILLAVITKDAKGNFQPVALRNLSAP
                     GKPLTFSPPPFDAMYSYYAPGPVGDFDRDGRVDIFLPSWFEDVPNLLFKNTTPGGHWL
                     TVAVRGADAKHNPQGIGAIVRAYEPGHVGDPQHQITRGDIVVGAGYASGEEALAHLGL
                     GDHQEVDLRITWGAEHRDRPGVAVDRLLSVDFPVR"
     gene            2738..2926
                     /locus_tag="VFV87_12670"
     CDS             2738..2926
                     /locus_tag="VFV87_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5104663.1"
                     /translation="MKQHILQFLLDEDGATATEYAVMLALIIGVCLASIGFFGSEAGG
                     SFSSTSSQIDTFMKGASS"
     gene            3041..>4018
                     /locus_tag="VFV87_12675"
     CDS             3041..>4018
                     /locus_tag="VFV87_12675"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5104664.1"
                     /translation="MTETTSTGLVQQFIRQTERLYSLPAVAMEALRLASEPHVDAVAL
                     KQCIERDPALTARILRVVNSSLFAASRQVTDLNQALSLLGTRPLKMLVLGFSLPKELF
                     TGIEADVLARYWRRTLTKAAAARELAQRLWRLSGDDAFTAGLVQDIGVLVLVQQLGEP
                     YLHLLDHVQVHGGNLLERELDSLGFDHRVLGARLLAHWGLPAGLCAAVAVPPNLERVE
                     ALEPADRTLPQILHLAELLARMLEQPYGTALRDLLAAGAKYRGLTYEGLRPIVAVLQR
                     QVTELADVLSLELPEQQSYADLLIAAQERLANETACATAELLSADPEAEL"
BASE COUNT          680 a         1243 c         1348 g          747 t
ORIGIN      
        1 aacgaacgcc ctctcgggcg aagccgtgat tcagaccttc gaagcgctcc gctgcaacct
       61 gggattgatc ggcgtttccg gcgtagcgcc gagcggaggg ctattcgtca agcaccgctg
      121 ggaagccaag taccgggccg cgattgtgcg gacggtctcg aatgagatct acatctgcac
      181 cgacgtcgaa aagctgggaa atcacgacct ctggcaattc gccacgatcg acgagatcgt
      241 cgccgacaag cccaagagcc gcgtggtgct gattacgaac aagccccgcg agctgcgcga
      301 acaccgcctc aaggtgcgtg ccgaggagac gctccgggag ctaaccgcga tctcgaaacg
      361 gctgggcccg caacggttcg aagtggtggt ggtgtagccg ccgaaatcgc gttcggctag
      421 cgaactggaa aatcgacgct cagaagccgg tcgacagcca cgccgggccg atcgcgatgt
      481 tccgcgcccc aggtgatccg gagatcgacc tcttggtggt cgcccaggcc aagatgggcg
      541 agggcttctt cgccgctggc gtagccggcg ccgacgacga tgtcgccgcg cgtgatctgg
      601 tgctggggat cgccgacgtg gcccggttcg taggcgcgga cgatggcgcc gatgccctga
      661 gggttgtgtt tggcgtcggc gccgcggacg gcaacggtca gccagtggcc acccggcgtc
      721 gtgttcttga agagcagatt cggcacgtcc tcgaaccagc tgggcaggaa gatatcgacg
      781 cggccatcgc ggtcgaaatc gcccaccggc ccgggggcgt agtagctgta catcgcgtcg
      841 aacggcggcg gcgagaaagt aagcggtttg cccggcgcgc tcaggttacg aagcgcgact
      901 ggctggaagt tgccctttgc gtctttcgtg atcacggcga gcaggatgtc gattttgctg
      961 tcgttgtcga gatcgcggag ggcgacgtga gccgtcttga ccggatgctc ctttgtgccg
     1021 ctgggaaagg tcgcgccgag gccgacggcg tcgctcacat cggcgagccg cggaatcccg
     1081 tccttgatcg ccgtggtgcg aaagacgtgc aggcgcgttg gcacgccatg ctcggtcacg
     1141 acgaggtcca ggagatcgtc gccatccagg tcggccagcg ccgcgccgca gcgcaggccc
     1201 tcgcgggcgt cgagcagcgg atattgcagg gccggattct cgacctcgcg atacttgccc
     1261 ccctttgctt gcgagacgaa caagcgattg ctgccggcga cgaagatgtc gatccggccg
     1321 tcgttgttca tgtcgcccag cgccaggccc aggccgggag tcttatcgag cggaaagccg
     1381 tagtcggcgg tcatgtcggc gaactgaaac tggccgccgt tctgcagtac gcgtagccgt
     1441 gaagtcgtgg cggcatcgcg gccataggag ccgtcggtca gcaccaggtc gagcagtccg
     1501 tcgtcgttaa agtcgattcc ggcgacgttc cgcatgccga tcacctcgtt ccagggagcg
     1561 cccggcggcg tcacatcgcg gaacttgcct gcgccgaggt tctcaaacac gcggctgccg
     1621 ccctggtcct tggtgacgtg attggtgacg accaggtcca gatcgcgatc gttgtccaga
     1681 tcgagaaaag ccgccccggc gcagcgggaa tcggcggcgc gggcggtcaa ggtatcgtcg
     1741 ctggcgggat tcagcttgcc atcctggttg aggaacagcc ggttgggcag cggggcgtcg
     1801 tcgccgttga accaggcccg ggcggcgaac gcgccgagga ataggtccgg ccggccgtcg
     1861 ttatcgacat cgccccaggc ggcgccgtga gcgtagcgcc agggccgcgt tttctcgtcc
     1921 ccttccaggt gctgtttcag gccggtcgcg gcagagactt ctgcgaacga gaacggctcg
     1981 gcggcggaaa gcgggaggga cagcgcccac gcgaagaatg aacagctcag gacggaggtg
     2041 actcgcatgg aatgggctcc tgtagtgatt caatggttgt agcgccgcag ataggagaaa
     2101 gcaaccttgc gactctcttc ggtacgaccg gcacaacatg atttggacgc atcgtagttg
     2161 ccgcgagacg gtcccgagca cgatagctag ctgcagcagg cgcttctagc atcgttgcct
     2221 cgcgcaaggt ggttcgcaat gacaacgaaa ataacattcc gtgcaacctt aatgaccgta
     2281 gcgattctgc tgccggcggc gatggcggcg ccggcctggg ctcacggtca tgaccacttg
     2341 ggccggcatt gcggcctggg ctggggtgat ggatatcact cgcgtacggc ctgcccgacg
     2401 cggaggtcgc tctctggcga gccgtgccct acgtgtgctc ctgtcgccgg tccggccatg
     2461 tacagcggcg cgttcgaatt cgcgccgacc atcgcaccga catctgcacc gcctgcgtat
     2521 cgtatgtcga aggctcccac cggaaccctt ttgccgcatt gggacaaaat gccggtgcgg
     2581 gggcaataat cgtttgcctc cccctcgttc gcaatctagg tttgaagtgc ggcacagtgc
     2641 ccgcggcgac ttgctcctcg tcaggcttcg tagcctcgcc tgacgcgtct ccgctgctgc
     2701 tgacagtcgc ggcgcggttc gattgggggc tggttccatg aaacaacaca tcttgcaatt
     2761 cctgctagac gaagacggtg caacggcgac cgaatatgcg gtcatgctgg cgctgatcat
     2821 cggcgtgtgc ctggcgtcga tcgggttttt cggcagcgaa gccggcggca gcttctcgag
     2881 cacctcttcg cagatcgata cgtttatgaa gggcgcatcg agctagtccg cttgtccttg
     2941 gtcatttgtc gttctcattc ggttcagaca aaggacaagt gaccaatggc ccttgccgta
     3001 agctacgttt gcgcagccgc tttctgcacg gcggtgcgct atgactgaaa ccacttcgac
     3061 cggcctggtc cagcaattca tccgccagac ggagcggctg tattcgctcc ctgcggtggc
     3121 gatggaagcc ctccgcctgg cgtccgagcc gcacgtcgat gccgtcgccc tcaaacagtg
     3181 catcgagcgc gatccggccc tcaccgcccg cattctgcgg gtcgtcaaca gctcgctctt
     3241 cgccgccagc cggcaagtca ccgatctgaa ccaggcgttg tcgctgctcg gcacacggcc
     3301 gctgaagatg ctcgttctgg gcttcagcct gccgaaggaa ctgttcacgg gcatcgaggc
     3361 cgacgtgctg gcccgctatt ggcggaggac gctcaccaag gccgccgccg cccgcgaatt
     3421 ggcgcagcga ttatggcggc tgtcgggcga tgatgccttt accgcggggc tcgtgcagga
     3481 catcggcgtg ctcgtgctgg tccagcagct cggcgaacct tacttgcatc tgctcgacca
     3541 cgtgcaggtt catggcggca acctgctgga gcgcgagctc gattcgctcg gcttcgatca
     3601 ccgcgtcctc ggcgcacgct tactggcgca ttggggtttg ccggccggat tgtgtgccgc
     3661 agtggcggtg ccgccgaacc tggagcgcgt cgaggccctg gagccggccg accggacgct
     3721 gccgcaaatt ctgcatctgg cggagctgtt ggcccggatg ctggagcagc cgtatggcac
     3781 ggcgctgcgg gatttgctgg cggccggcgc caagtatcgc gggctgacct acgagggact
     3841 gcggccgatt gtggccgtct tgcagcggca ggtgaccgag ctggccgacg tcttgtcgct
     3901 ggagctgccc gagcagcaga gctatgccga cctcttgatt gccgcccagg agcggctggc
     3961 gaacgaaacg gcttgcgcca cggcggagct gctttcggct gatcccgaag cggagctg
//
LOCUS       DASUGB010000664         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1185820, whole genome shotgun sequence.
ACCESSION   DASUGB010000664 DASUGB010000000
VERSION     DASUGB010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185820"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFV87_12680"
     CDS             complement(<1..516)
                     /locus_tag="VFV87_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104665.1"
                     /translation="MTCSPAAPKAARQTVVAVLRRQVQRLEKSLPLADDRPLSTGSPA
                     LDALLPGGGLRRGSLVEYLSPGAGSGTGSLALCAAREACAEGRALVVVDRRRTFYPPA
                     AVTWGFDLANLLMLQPADDAAELWAIDQALRCPGVGAVYAPCGALDVRDFRRLQLAAE
                     AGGTLGILLRPN"
     gene            complement(710..1651)
                     /locus_tag="VFV87_12685"
     CDS             complement(710..1651)
                     /locus_tag="VFV87_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX5104666.1"
                     /translation="MVRPWVAVLTSFHNTPIFGRIWLTVAAFGLYAAGVIAIDYFFLG
                     GIVTVGPEIHGFLGLILGTLLVFRTNTSYDRWWEGRKLWGQLVNDSRNLAIKVQTCVR
                     SGEADKQRLGQWLIDFAYALRDHLRGGAKLRDRKSFPDVAAEPPHVPAYISARIYDQF
                     EAWRQDDKLGGFELLFLDQHASSLMNICGACERIQKSPISVSYRWFIRQLIAIYLITL
                     PWGLIEQFGWWTDPAVVMLAYFMIGVEMIAEEIEDPFGTSEDDLLLDDLCHTIERSVN
                     GILADPVLAPEAGPPGGWKQVPPVVLPTPDSSLSDSP"
     gene            1803..>2267
                     /locus_tag="VFV87_12690"
     CDS             1803..>2267
                     /locus_tag="VFV87_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5104667.1"
                     /translation="MVQQLVAGVHQFKHEVFRHQRELFERLAKGQTPQTLFITCSDSR
                     IDPNLITHTDPGDLFVLRNAGNIVPAYGAGSGGELATIEFAVTGLEVSDIVVCGHSHC
                     GAMKGLLHPEYLEEMPAVAEFLKHAEATRRIVKSRYADLHGEPLLDATIEENI"
BASE COUNT          477 a          734 c          681 g          375 t
ORIGIN      
        1 attcgggcga aggaggatac ccagcgtgcc gccggcttcg gcggcgagtt gcaggcggcg
       61 aaagtcgcgg acatccagtg ccccgcacgg agcatacacc gcgccgacgc ccgggcaacg
      121 cagcgcctga tcgatggccc acagctcggc cgcatcatcg gccggctgga gcatcagcag
      181 attcgccagg tcgaagcccc aggtaacagc cgcgggcgga taaaacgtgc gtcggcgatc
      241 gaccaccacc agcgccctgc cctcggcgca tgcttcgcga gcggcacaga gggcgagcga
      301 tcctgttccg ctccccgcgc ccggcgagag atattccact agcgagccgc gccgcagccc
      361 accccccggc aagagcgcat ccagcgccgg ggagccggtc gaaagcggcc ggtcatcagc
      421 cagcggcagc gatttctcca gccgctggac ttgccggcgg aggacggcga ccaccgtctg
      481 gcgggctgcc ttgggggcgg ccggagagca cgtcatggct cacaccttct gaacagtata
      541 gtatatccaa gagactacca aaactgtaca gccgatccaa aaacgcgtca agctagcatg
      601 tcactagttt gtacagaatt cagaagttta gtacttttat gtcaacgtgc tttgcgtaca
      661 gtatctaaca ggccacaaaa taccgacttt aggaggactc aatgcaccgc tacggactat
      721 cgctcagcga agaatccggc gtcggcagca ccacgggcgg aacctgcttc cagcccccgg
      781 gcggacccgc ctccggcgcc aggaccggat cggccagaat gccgttcacg ctccgctcga
      841 tcgtgtggca caggtcgtcg agcagcaggt cgtcttcact ggtcccgaac gggtcctcga
      901 tctcctcggc aatcatctcc acgccgatca tgaaataggc cagcatcacc accgccggat
      961 cggtccacca gccgaactgc tcgatcagcc cccagggcag cgtgatcagg taaatcgcga
     1021 tgagctggcg gatgaaccag cggtacgaca cggagatcgg cgatttctga atccgctcgc
     1081 aggccccgca gatattcatc agcgacgacg cgtgctgatc caggaacagc aattcaaacc
     1141 cgcccagctt gtcgtcttgc cgccaggcct cgaactgatc gtagatccgg gcggagatat
     1201 acgccggcac atgcggcggc tcggcagcca cgtccggaaa cgacttgcgg tcgcggagct
     1261 tcgccccgcc gcgtaaatgg tcccgcaggg cataggcaaa gtcgatcagc cactgcccca
     1321 accgctgctt gtcggcctcg ccggagcgca cgcacgtctg caccttgatc gccagattgc
     1381 ggctgtcgtt gacgagttgc ccccacagct tgcgtccctc ccaccagcgg tcgtacgacg
     1441 tgttggtgcg aaagacgagc agcgtgccga gaatcaggcc cagaaagccg tggatttccg
     1501 gccccacggt cacaatcccg cccaaaaaga aatagtcgat cgcaatgacc cccgccgcat
     1561 acaagccgaa cgccgccacc gtcagccaga tgcggccgaa gattggcgtg ttatgaaacg
     1621 acgtcagtac ggcgacccag ggacggacca ttcgattttg gattgcggat ttcggattgc
     1681 ggatttgggc gcgaaccgat tacccgccag gtggagagaa agacccaagc cgggtacatt
     1741 gcgggcgtcc gattcgcgga cgctcaaaat agctgatttt tctgccagac gtgaggccag
     1801 ccgtggtcca gcagctcgtt gccggcgtac accagttcaa gcacgaggtt ttccgccacc
     1861 agcgggagct attcgagcgt ttggcaaagg ggcaaactcc ccagacgctg ttcatcacct
     1921 gctccgattc gcgcatcgac ccgaacctga tcacgcacac cgatccgggc gacctgttcg
     1981 ttttgcggaa cgccggcaat atcgttccgg cttatggcgc cggcagcggc ggcgaactgg
     2041 cgaccatcga gtttgcggtg acgggactgg aggtgagcga tatcgtcgtc tgcggccatt
     2101 cacactgcgg ggcgatgaaa gggctgctcc acccagaata tctggaggag atgccggccg
     2161 tcgcggagtt tctcaagcac gccgaggcca cgcgacggat cgtgaaatcg cggtatgccg
     2221 atctgcacgg cgagccgctc ctggatgcga cgatcgagga aaacatc
//
LOCUS       DASUGB010000665         4532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_629778, whole genome shotgun sequence.
ACCESSION   DASUGB010000665 DASUGB010000000
VERSION     DASUGB010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629778"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFV87_12695"
     CDS             complement(<1..452)
                     /locus_tag="VFV87_12695"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEX5104668.1"
                     /translation="MKNHWYCGVEGQQYGPFTWEQLRAMAAEGRLIGESFVRREVDQQ
                     WFQASQIPGLLPKAHPAKKNGSSASSIVKAGAKVSESGALAAVSADPSKSSSTSRRAK
                     ARPAGTASQSSGNIPVGKPAAARSAPVAVGTATPSQGFAFTPAVNTAAS"
     gene            complement(562..1002)
                     /locus_tag="VFV87_12700"
     CDS             complement(562..1002)
                     /locus_tag="VFV87_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5104669.1"
                     /translation="MPTDPEPSRSAWPRLWLRRADQAVAAVFIAVALAAIGGYTLWQG
                     SIRSRLIEIDRAEPIAIETKIDINRADWPELCLMPGVGEVLAQRIVADREENGPFLEL
                     DDLRRVRGIGPLTLEGMKPYLLPMPELDATAEGNSGGRGAKLVN"
     gene            1119..2096
                     /locus_tag="VFV87_12705"
     CDS             1119..2096
                     /locus_tag="VFV87_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104670.1"
                     /translation="MSEHERQQQLEYPYTARPAAGALEEDSGTLPLDWLAGPTWLGLI
                     GVAILFALANLTIFALMENISSRDLAAPLVYWLFGAIAAQPAVLSAWLVWGSGSFWKR
                     LALHWGAAYGLAILWLIGAVAAEGPRDGDIRNALEIVPFSLPLLGLAIQLPLWAARMG
                     FGWRLVDSCADDPRPRPLAIRDLMVGTVIVAVSLAAAKLAEGMDRGLEGWLVWAILAP
                     SVAGISLIAVLPVAAWILRQRNLVMGMVLTAVYAAIAVAVTWVVMYVIERNFFWVNWR
                     DMLGLAVTIFSFAATLAVAALAARVAGFRLATGHSAPVQSADPHPGDRT"
     gene            2093..3082
                     /locus_tag="VFV87_12710"
     CDS             2093..3082
                     /locus_tag="VFV87_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104671.1"
                     /translation="MTEPELPAEPKSEPEQNSFADRPDAKGPLGPAFHRTAAAETNSN
                     ARQSAIVIIFILLGVFALVNCAGVPFTEEVNGGPGSGVEFVFAIGIGIVGGQIGSLTG
                     ALVWSNAPFLLRLMGLWLLALLLFGCWFAGLAIAMQENEWMSVIMYDVARGIAASLPL
                     ISLAIQLPQWVVRLYFGWRIEPRAADAAARPRQALAIRDILIGTAIVALTLTAVRLAV
                     RDRDQLGPEFWVAWAVAFGVLLVVSAVVNLPLVLLILRARSPAGAVLFLLFGPSALVV
                     VAILIAMIFEPGGPDARSVMLMMLAATTCVAVSSAPLWLYRLTGHRLKMAREG"
     gene            complement(3105..>4532)
                     /gene="mutS"
                     /locus_tag="VFV87_12715"
     CDS             complement(3105..>4532)
                     /gene="mutS"
                     /locus_tag="VFV87_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5104672.1"
                     /translation="DLCPEIHTQLQSALVEDCPLSAKEGGIIRGGYSGDLDKLRDLAT
                     GGKQWIAKYQASQAEATGIANLKVAYNKVFGYYIEITNSQKDKTPAHYIRKQTTVNAE
                     RYITPELKEYEEQVVTADERAKELEYELFVSLRDTVQREAKRLQTTAAALAQLDVLAS
                     LAELARQRGYIRPTMTAEPILQITDGRHPVLDITEPQGTFVPNDVRCGGEDGTVLLIT
                     GPNMAGKSTYIRQVALLTLLAQMGSFVPAREATIGIADRIFARVGASDELSRGQSTFM
                     VEMTETARILNTATPRSLVVLDEIGRGTSTYDGISLAWAIVEHLHDRVGCRTLFATHY
                     HELTDLAQTLAGVRNLNVAVKEWQDHVVFLHKIVPGAADKSYGIHVARLAGVPREVNE
                     RAKQILAQLEAEHLGSDGRSKIAIHGKHTRRGDVQLTLFAPPEHPALGQLRQLDLERL
                     TPLEALQRLAELQAQVEPAVSRKPR"
BASE COUNT          639 a         1453 c         1555 g          885 t
ORIGIN      
        1 gacgctgccg tattcacggc aggcgtgaag gcgaatccct gcgagggcgt cgcggtgccc
       61 acagcaaccg gcgcggatcg cgcggccgct ggctttccaa cggggatatt gcccgacgat
      121 tgcgaagccg tcccggccgg ccgcgctttg gcccgccggc tcgtgctgga tgatttcgac
      181 ggatcggcgc tgaccgcggc cagcgcgccc gattccgaga ctttggcgcc ggccttgacg
      241 atcgacgagg cgctgctgcc gttcttcttt gccggatggg ccttcggcaa caggccagga
      301 atctgcgacg cctggaacca ctgctgatcg acttcgcggc ggacgaacga ctcgccgatg
      361 agccgcccct cggcagccat cgcccgcagt tgttcccagg tgaaagggcc gtactgctgc
      421 ccttcgacgc cgcaatacca gtggtttttc acaagccgtc ctccggcaca cagtaggctc
      481 cgacggccgc ggcggaacca gatgcgtgat ggactcaatg ccgccaggtc ccgcccaggc
      541 ggtcgggacc tacaccgccc ctcaatttac tagcttcgcg ccccttccgc cagaatttcc
      601 ctccgcggtc gcgtccagct ccggcatcgg caacaggtag ggcttcatcc cttcgagcgt
      661 gagcggcccg atgccacgga cccgccgcag gtcgtccagc tccaaaaacg gcccgttttc
      721 ctcgcgatcg gcgacgatcc gctgggccag gacttcgccc acgccgggca ttaaacaaag
      781 ctccggccag tcggcccggt tgatgtcgat cttggtttcg atcgcaatcg gctcggcgcg
      841 gtcaatttcg atcagccggc tgcgaatgct cccttgccag agggtatatc cgccgatggc
      901 tgccagcgcg acggcgatga agaccgcggc caccgcctga tcggcccgcc gcagccaaag
      961 ccgcggccag gcggagcggg atggttcagg gtcggtgggc atcggataac tgtcgtcgtt
     1021 cgctccgcga acgaacgcgc cctttcgcgg agcgaaaggc gacgatagga agcggtcggg
     1081 gtaagatacg agcctgcgcg gcaccagacg ggttcattat gtccgagcac gagcgccaac
     1141 agcaattgga atacccctac acggcccgtc ccgcagccgg cgcgctggag gaggattccg
     1201 gcacgctgcc gctcgattgg ctggcggggc cgacgtggct ggggctcatt ggcgtggcaa
     1261 ttctgttcgc gctggcgaac ctgaccatct tcgccttgat ggagaacatc agctcgcgcg
     1321 acctggcggc gccgctcgtc tattggttat tcggcgcgat cgccgcgcag ccggccgtct
     1381 tgtcggcgtg gctggtgtgg gggagcggat cgttctggaa aaggttggcg ctgcactggg
     1441 gcgcggcgta cggcctggcg atcctgtggc tgatcggggc ggttgccgcc gagggacctc
     1501 gcgacggcga cattcgcaat gcgttggaaa tcgtcccctt ctcgctgccg ctcttgggac
     1561 tggcgattca gcttccactg tgggccgccc ggatgggctt cggctggcgg ctggtggatt
     1621 cgtgcgcgga tgatccacgg ccccggccgc tggcgattcg cgacctgatg gtgggcaccg
     1681 tgattgtcgc ggtctcgctg gcggcggcga agctggccga gggcatggac cgcggcttgg
     1741 aaggctggct cgtctgggcg attctcgccc ccagcgtcgc cggcatcagc ctcatcgccg
     1801 tcctgccggt cgccgcctgg attctgcggc agcggaacct cgtgatgggc atggtcctga
     1861 cggccgttta tgccgccatc gccgtcgccg tgacgtgggt cgtcatgtat gtcatcgagc
     1921 gcaacttttt ctgggtcaac tggcgggaca tgctcggcct cgccgtcacg atcttcagct
     1981 tcgccgccac cctcgccgtc gccgccctgg cagcgcgggt ggcgggattt cgcttggcaa
     2041 ccggacactc ggctccggtg cagtcagccg atccgcatcc aggggaccgc acatgaccga
     2101 gcccgagctc ccggcggagc caaaatccga gccggagcaa aattcctttg ccgatcgtcc
     2161 tgatgcgaaa ggcccgctgg gacctgcttt ccatcggacg gcggcagcgg aaacgaactc
     2221 caacgcccgc cagtcggcga tcgtgatcat cttcatcctc cttggcgtct ttgccctcgt
     2281 gaactgcgcg ggcgtgccgt ttaccgagga agtcaatggc ggccctggca gcggcgttga
     2341 attcgtgttc gcgatcggca tcggcattgt cggcgggcag atcggctcgc tgacgggcgc
     2401 gctggtgtgg agcaatgccc cgttcctctt gcgcttgatg ggcttatggt tgctggcgct
     2461 cttgctgttt gggtgctggt ttgccggtct ggcgatcgcg atgcaggaaa acgagtggat
     2521 gtccgtcatc atgtacgacg ttgcccgggg aatcgccgcc agcttgccgc tgatcagcct
     2581 ggcgatccaa ttgccgcagt gggtggtgcg gctctatttc ggctggcgaa tcgagccgcg
     2641 agccgccgat gccgctgcgc ggccccgcca ggcgctggcg atacgcgaca tcctgattgg
     2701 tacggcgatc gtcgccctca cgctaaccgc ggtgcgcttg gcggtgcgcg accgcgatca
     2761 actcggccca gagttttggg tggcgtgggc ggtcgcattc ggcgtgctgc tggttgtcag
     2821 cgccgtcgtc aacctgccgc tggtgctgct gatcctgcga gccaggagcc ccgccggcgc
     2881 cgtgctgttt ctcctgttcg gcccgtcggc cctcgtggtc gtcgcgattc tcatcgcgat
     2941 gatattcgag cccggcggcc ccgacgccag atcggtcatg ctgatgatgc tggccgcgac
     3001 cacgtgcgtt gccgtgtcat ctgcgccgct gtggctctac cgactgaccg ggcaccgcct
     3061 gaaaatggcg cgcgagggct gagcggcgcg ctagctgttt ccgttcagcg cggcttgcgt
     3121 gataccgctg gctccacctg ggcctgcaat tccgccaggc gctgcaaggc ttccagcggt
     3181 gtgaggcgct caaggtcgag ctggcggagc tgacccaagg ccggatgctc gggcggggcg
     3241 aagagggtga gctgcacgtc gccgcggcgg gtgtgcttgc cgtgaatggc gattttggag
     3301 cggccgtcgc tgcctaggtg ctcggcttcg agctgggcca ggatctgctt ggcccgctcg
     3361 ttcacttcgc ggggcacgcc cgccagccgg gcgacgtgga tgccgtagct cttgtcggcc
     3421 gcgccgggca cgattttgtg caggaatacg acgtggtcct gccattcctt gaccgcgacg
     3481 ttcagattcc gcacgccggc cagcgtctgc gcgagatccg tcagctcgtg gtagtgcgtg
     3541 gcaaagagcg tgcggcagcc cacgcggtcg tgcaggtgct cgacaatcgc ccaggcgagc
     3601 gaaatgccgt cgtaggtgct ggtgccgcgg ccgatttcgt cgaggacgac gaggctccgc
     3661 ggcgtggcgg tattgaggat gcgggccgtc tcggtcattt cgaccatgaa tgtgctttgc
     3721 ccgcgcgaga gctcgtcgct ggcgccgacg cgggcgaaga tccggtcagc gatgccgatc
     3781 gtcgcttcgc gagccgggac gaagctcccc atctgggcca gcagcgtcag cagggcgacc
     3841 tgccggatgt acgtgctctt gcccgccatg ttcggcccgg tgatcagcag cacggtgcca
     3901 tcctcgccgc cgcaccgcac gtcgttgggg acgaacgtcc cttgcggctc ggtgatgtcg
     3961 agaaccggat gacggccgtc cgtgatctgc aggatgggct cggccgtcat tgtcggccgg
     4021 atgtagccgc gttggcgggc cagctccgcg aggctcgcga gcacgtcgag ctgcgcgaga
     4081 gctgcggcgg tcgtttggag acgcttggct tcccgctgca cagtgtctcg cagagaaaca
     4141 aacagctcgt actccagctc cttggcccgc tcgtcggcgg tgacgacctg ctcttcgtac
     4201 tctttcagct cgggcgtgat gtagcgctcg gcattgacgg tcgtctgctt gcggatgtag
     4261 tgggcgggcg ttttgtcctt ctggctgttg gtgatctcga tgtagtagcc gaagaccttg
     4321 ttgtaggcga ccttgaggtt ggcgatgccg gtggcctctg cctggctcgc ctggtatttg
     4381 gcgatccact gcttgccgcc ggtcgccagg tcgcggagct tgtcgagatc gccgctgtaa
     4441 ccgccgcgga tgatcccgcc ctccttggcc gagagcgggc agtcctcaac gagggccgac
     4501 tggagctggg tgtggatttc ggggcagagg tc
//
LOCUS       DASUGB010000666         6918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1742596, whole genome shotgun sequence.
ACCESSION   DASUGB010000666 DASUGB010000000
VERSION     DASUGB010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742596"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..991
                     /locus_tag="VFV87_12720"
     CDS             200..991
                     /locus_tag="VFV87_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5104673.1"
                     /translation="MSNDRNAKTWAFGVEPGRLPYRLRQARYQALGEDVGRLAREHFD
                     RTRRALELIDVGTDEGVARRYIEAQPGGERVRYHGVDLFPEGMRNVYKRHDWKLTQID
                     LEGGMPGIAPDRYDVVICEQVLEHLHHCEEALGELVRVLRPGGMLIVGVPIFPHGIHA
                     LRQPLVNTLAALKLRTNSPHGHVQAFSKRSFLRLLEGTGELEIQEVRGFRIISGGPLR
                     GLEYCRWWWLLNRRIGRIVPSLCVEIQVVARKKLRAAEVAVRAAA"
     gene            complement(1042..1356)
                     /locus_tag="VFV87_12725"
     CDS             complement(1042..1356)
                     /locus_tag="VFV87_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5104674.1"
                     /translation="MNKADSSKECADRLKALADPDRLRIVQILRSGPKNVSEITAEMK
                     AEIANVSHHLQILKRERLVETEKQGRFVVYRLHPDVCEPTPDSGSLDLGCCRLQWPEE
                     RK"
     gene            1502..2350
                     /locus_tag="VFV87_12730"
     CDS             1502..2350
                     /locus_tag="VFV87_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX5104675.1"
                     /translation="MPSTFRTLLAETLGTFLLVLFGCGAVHAAVLTGAQSGLWQVAIV
                     WGMAVMFAVYAVGAVSGAHINPAITVAFAVWGRFPWGKVVPYIVAQLGGAMLAASALF
                     VIYGPNLAAKEREKGITRGQLGSIVTAMCYGEYYPNPAPIAALPEKWSDSAWAEYATR
                     VTFTQAFVAEVLGTAVLAFMVFAVSDGKNAGAPPSYLAPVFIGLTVAALISIIAPLTQ
                     ACLNPARDFGPRLFAFIAGWQEAAIPGPNGHGIFTVYIAAPILGGILGGGLYDWLIKP
                     AFGETK"
     gene            2347..3528
                     /locus_tag="VFV87_12735"
     CDS             2347..3528
                     /locus_tag="VFV87_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5104676.1"
                     /translation="MIKIQRTEEPRTRHLACRVLALWFFGSCFLDFSAAADLDPALPY
                     QAERSSPVTYDVDFSVVVTPPYHTHVLKVWLPLPQSDAAQDVSESRLSTFPLEVRPAI
                     ARETQFGNQFAYFEFHEPQGAQIVRHQFCVKSWELRWKLDPEKIQAVKEWPASFDRYR
                     RSESQAVQVDERFHDLLRTIVPQSRGGLQDLAAVMRWSTEHLTYDHHDASLRADSVRM
                     LEQQRGHCSDYHGFCAAMGRALGYPTRVAYGINTFPKNSPSHCKLEAFLPPYGWVSFD
                     VSETQKLVHAIRKDAKLSDDEKANLAKAATARLTSGFRDNTWFCLTRGTDYDLAPPAS
                     KRVPVVRTAYIEADGVPLPDPDPADPERREFAWMTVHKYTPDRQVPYPFKDFRTLNPE
                     P"
     gene            3537..4640
                     /locus_tag="VFV87_12740"
     CDS             3537..4640
                     /locus_tag="VFV87_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5104677.1"
                     /translation="MSIRFVMIGGFLGAGKTTTIARLARHYQDQGLKIGIVTNDQATD
                     LVDTNSLRAQGFDVGEVPGACFCCNFNKLTETVGQLRDSARPDLVLAEPVGSCTDLVA
                     TVVRPLQQLYGGTFDVAPYAVLLKPSHGSRILRGQAKGGFSPQAAYIFKKQLEEADLI
                     VINRIDELSADEADVLAGLLQTQYAGTPVLRMSAKTGNGFDALVEMLDQRGRFGQRIM
                     DVDYDTYAVGEAELGWLNSSLSVSAPKPLDLDALLLDIVARLQASLAEIDAETAHLKT
                     IGMADGLYGVANLISSFSPPELSLPSRGQVRAADVVVNARVATAPETLTEHVERAVYA
                     AAAAKGATAAFQQTQSFRPGRPVPTHRILEPTA"
     gene            complement(5028..5510)
                     /locus_tag="VFV87_12745"
     CDS             complement(5028..5510)
                     /locus_tag="VFV87_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104678.1"
                     /translation="MLRPKSSCVLWSASGVCGLLAVAAIAFAQHEGALPAAGQKSLSE
                     LTPAAGKAVQRQREGSLLTAEIGAFELVGDRVVFVPAGSRESFRVLENLALERVLREL
                     GDARDQRNWSVWGQFTEFRGANYLLVTKALVKTAPGPAASRREQSAATNPVERNDGGR
                     "
     gene            5862..6692
                     /locus_tag="VFV87_12750"
     CDS             5862..6692
                     /locus_tag="VFV87_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104679.1"
                     /translation="MSAADGELVVRVASGREFTGLFDDRSDSQSLVLRSQRGSVTIHR
                     PIEWDRIVSATKDGQAVTIDALRQSIAMPVPPAPASDVRTIIVESREPVAGDDEVVQA
                     TALEPLPPVTTITFDARIANWDWDVETDGVVLDLLPLDGDGYLAPASGTVQVELYAPQ
                     RRRFDLAPQSGGDTLELVERWTRAIYAADFTTSGVRLRLPFGAIHPEFDSDWLAWQYG
                     LVHVRFAAPGHGTFDSSQDGIRMRPWAPNRDQLELNTRRRFLPTEATGRPKESIGLHK
                     "
     gene            complement(6773..6916)
                     /locus_tag="VFV87_12755"
     CDS             complement(6773..6916)
                     /locus_tag="VFV87_12755"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104680.1"
                     /translation="MGDESISIDEVAVSSGLPIHNVLATISALEMRRLIRRVGGSQVR
                     RAY"
BASE COUNT         1186 a         2292 c         2182 g         1258 t
ORIGIN      
        1 gtcgggagtc aattgcggct aacgtcccac ggtgtggacc atcgatgacc gcaattgact
       61 cccgaccccg ttcgcgcggg cgaactatcg caaattagtt gcgatccgtc gagatgagtg
      121 ccccgctagc agcgcccgca acatcgctct tgtccgcaac gggctggcgt cggtagtctt
      181 cgcaccggga ggactgccaa tgtcgaatga tcgcaacgcc aaaacgtggg cctttggcgt
      241 cgagccaggc cggctgccgt atcgcttgcg tcaggcccgc tatcaggcgc tgggcgagga
      301 cgtggggcgg ctggcccgcg agcacttcga ccgcacgcgg cgggcgctgg aattgatcga
      361 cgtggggacc gacgaaggcg tggcccggcg gtacatcgag gcccagccgg gcggcgagcg
      421 ggtccgctat cacggcgtcg atctgttccc cgaggggatg cggaacgtct ataagcggca
      481 cgactggaag ctgacgcaga tcgacctgga aggcgggatg ccgggcattg cgcccgatcg
      541 ctacgacgtg gtgatttgcg agcaggtgct ggagcatctg caccattgcg aggaggctct
      601 gggcgagcta gtgcgcgtgc tgaggccagg aggaatgctg atcgtcggcg tcccgatttt
      661 tccgcacggc atccacgcgc tccgccagcc gctggttaac acgcttgcgg ccctgaagct
      721 ccgcaccaac agtccccacg gccacgtgca ggcgttttcg aagcggagct tcctgcgact
      781 gctggagggg acgggcgagc tggagattca ggaggtccgc ggctttcgga ttatctcggg
      841 cgggccgctg cgcgggctgg agtattgccg ctggtggtgg ctgctcaacc ggcggatcgg
      901 ccggatcgtg ccgtcgctgt gcgtcgagat tcaggttgtc gcgcggaaaa agctgcgggc
      961 agcggaagtt gcagtgcggg cagcggcata gagccaatcc gagggccctc accccggccc
     1021 tctcccaggg gagagggaga gctacttccg ttcttccggc cactgcagcc ggcagcagcc
     1081 gagatcgagg ctgcccgaat cgggcgtggg ctcgcagacg tcgggatgca ggcgatagac
     1141 gacgaaccgc ccctgctttt cagtctcgac gaggcgctcg cgcttcagaa tctgcaggtg
     1201 gtgggagacg ttggcgattt cggccttcat ctccgccgtg atctcgctga cgtttttcgg
     1261 gccgctgcgc aagatttgca caatccgcag ccggtctgga tcggccagcg ccttaagccg
     1321 atcggcacat tccttggacg aatcggcctt gttcatggcg gcggcggccc cagcagatta
     1381 ccaggcggac gactacaatt gccgtcacga ttgagaatat ccgcccggat gctgtttcgg
     1441 caaccgctgc gggcggcgct tgcgacgctg tcaaacggtc gttagagtat ctgagactcc
     1501 catgccatcc acctttcgca cactcctggc cgaaacgctc ggcacgttcc tgctcgtcct
     1561 attcggctgc ggggccgtac acgcggcggt gctgacgggc gcccagagcg gcctgtggca
     1621 agtggcgatc gtgtggggga tggcggtgat gttcgcggtc tatgccgtcg gcgccgtgag
     1681 cggagcccat atcaacccgg cgattaccgt cgccttcgcc gtgtgggggc gatttccgtg
     1741 gggcaaagtc gtgccctaca tcgtcgcgca gcttggcggg gcgatgttgg cggcgtcggc
     1801 cttgtttgtg atctacgggc cgaacctggc ggccaaggaa cgcgagaaag ggattacgcg
     1861 cggccagctc ggcagcatcg tgacggcgat gtgctacggc gagtactatc cgaacccggc
     1921 tcccatcgcg gcattgcctg agaaatggtc cgattcggca tgggccgagt acgcgacgcg
     1981 agtcacgttc acacaggcct tcgtggccga ggtgctgggg acggccgtcc tggccttcat
     2041 ggtctttgcc gtgagcgacg gcaaaaatgc cggcgctccg ccgagttacc tggcgccggt
     2101 ctttattggc ctcacggtcg cggcactcat atcgatcatc gccccgctga cacaagcctg
     2161 cctcaatccg gcccgcgact tcgggccgcg gctgtttgcg ttcattgccg gctggcagga
     2221 ggccgcaatt cccggcccca atgggcatgg cattttcacc gtgtatatcg ccgcgccgat
     2281 tctgggcgga atcctcggcg gcgggctgta cgactggctg atcaagccag cgtttggaga
     2341 gacgaaatga tcaagatcca aagaaccgaa gaaccaagaa cccggcacct ggcgtgtcgc
     2401 gtgttggctc tttggttctt cggttcttgt ttcttggatt tttcggcggc ggccgacctc
     2461 gatcccgcac tcccctacca agctgagcgg tcgagccccg tcacctacga cgtcgatttc
     2521 tcggtcgtcg tgacgcctcc ttatcacacg cacgtgctga aggtctggct gccgctgccg
     2581 cagtcggatg cggctcagga cgtttccgag agcagactct cgacctttcc gctcgaagtt
     2641 cgcccggcga ttgcccgcga aacgcagttc ggcaatcagt tcgcctactt cgaattccac
     2701 gagccgcagg gggcccagat cgtccggcac cagttctgcg tcaaatcgtg ggagctgcgc
     2761 tggaagctgg acccggagaa gatccaggcg gtgaaggaat ggcccgcgtc atttgatcgc
     2821 taccgccgca gcgaatcgca ggcggtccag gtcgatgagc ggttccatga tctgctccgc
     2881 acgatcgtgc cgcagtcgcg cggcggactt caggacctgg ccgcggtcat gcgctggagc
     2941 acggaacatc tgacctacga ccatcacgac gcctcgctcc gcgctgattc cgtcaggatg
     3001 cttgagcagc agcgcggcca ctgcagcgac tatcacggct tttgcgcggc aatggggcgg
     3061 gctttgggat atccgacgcg ggtcgcctac ggcatcaaca cctttccgaa gaattcgccc
     3121 tcgcactgca agctcgaagc cttcctgccg ccgtacggct gggtcagttt cgacgtttct
     3181 gagacgcaga agctcgtcca tgcgatccgc aaagacgcga agttaagcga cgacgagaaa
     3241 gccaatctgg caaaagccgc gacagcccgg ctgaccagcg gctttcgcga caatacctgg
     3301 ttctgcctca cccgcggcac cgactacgac ctcgcgccgc ctgccagcaa gcgcgtcccc
     3361 gtcgtccgca ccgcctacat cgaagccgac ggcgtcccgc tgcccgatcc cgatccagcc
     3421 gatcccgagc gccgcgagtt tgcctggatg accgttcaca aatacacgcc cgaccgtcaa
     3481 gtgccatatc cgttcaaaga tttccggacc ctgaaccctg aaccctgaac cctcccatgt
     3541 ccatccgctt cgtcatgatc ggcggcttcc ttggcgccgg caaaacgacc acgatcgccc
     3601 gcctggctcg ccactatcaa gaccaggggc tgaagatcgg catcgtcacc aacgaccagg
     3661 cgaccgacct ggtcgatacg aacagcctgc gggcccaggg gttcgacgtc ggcgaagtcc
     3721 ccggcgcatg cttctgctgc aatttcaaca agctgactga aaccgtcggc cagcttcgcg
     3781 attcggcccg gcccgacctg gtgctcgccg agcccgtcgg aagctgcacc gacctcgtgg
     3841 ccaccgtcgt gcgcccgctg caacagcttt acggcggcac gttcgatgtc gccccctatg
     3901 ccgtgctcct caagcccagc cacggcagcc gcatcttgcg gggccaggcg aaagggggtt
     3961 tctcgccgca ggcggcctac atcttcaaaa agcagcttga ggaggcggac ctgatcgtca
     4021 tcaaccgcat cgacgagctt tccgccgacg aagcggacgt gctggccggc ctgctgcaaa
     4081 cacaatatgc cggcactccg gtgctccgca tgtcggccaa gacgggcaac ggctttgatg
     4141 ctctggtcga gatgctcgac cagcgcggcc gcttcggcca gcggatcatg gacgtcgatt
     4201 acgacaccta cgccgttggc gaggcggagc tcggctggct gaacagcagt ttgagcgtct
     4261 cggctccgaa accgctggat ctcgacgcgc tgctgttgga cattgtagcc cgcctgcaag
     4321 cctcgctagc cgaaatcgac gccgaaacgg cgcacctaaa gaccatcggc atggccgacg
     4381 gcttgtatgg cgtcgccaac ctgatcagca gcttttcgcc gccggagctg tcgctcccgt
     4441 cgcgcggcca ggtgcgggcg gcggatgtgg tcgtgaacgc ccgcgtcgcg acggccccgg
     4501 aaacgctcac cgagcacgtc gagcgggcgg tgtacgccgc ggctgccgcc aagggggcca
     4561 cggcggcgtt ccagcagacg cagtcgtttc gccccggccg gccggttccc acgcaccgca
     4621 tcctggagcc gacggcgtga gttctgcttg atcttagcac agcaagccgg cgcaccgctt
     4681 ccacgtggtt ctaagtcccc aacgactgct gccgctcccc gcgcgtatct ggatcaaccc
     4741 agccgagccg cgtacggcct accctggaga gccacgactt tgaccttgac cgccatcctt
     4801 gcccctgcct tgctttccac tcttgaccag taccgacgcc gcaagcagca actcactcgc
     4861 tacggccaac gccccttcgg catctagccg gactgcggcc ttcgcccatg tcacgagagg
     4921 gtcgtgatcc gacccggtcg ctcgcacttg cacaacgatc tgtttcgcgt cgctgaacac
     4981 gtgcaggttg ccaccgccca gtgccgtccg gcgcatgcct ttcacgttca tcggccacca
     5041 tcgtttctct ctacgggatt cgtagcggcc gactgctcac gccgagaagc ggctgggccg
     5101 ggcgctgtct tcacgagggc cttggtcacc agcaggtagt tcgctccgcg gaactcggtg
     5161 aactgccccc acacgctcca attcctctgg tcgcgggcat cgcccagctc gcgcagcacg
     5221 cgttccagcg cgaggttctc cagcacgcgg aacgactcgc ggctgccggc gggaacgaac
     5281 acgacgcgat cgccgaccag ctcgaaggcc ccaatttcag cggtcagcag gctcccttcg
     5341 cgctggcgct gcaccgcctt gcctgcggcg ggcgtcaact ccgagagcga tttctgtccc
     5401 gccgcgggga gtgcgccctc gtgctgggca aatgcgatgg ccgcaacggc caacagaccg
     5461 caaacgccgg atgcactcca taagacgcag gacgatttcg gccgaagcat cgggcgactc
     5521 ctcgctgaga tcggggacgg cacctgcaac cctagctctg ctgacgcaag ggtgagcggc
     5581 cgcgacgcgg cgttgccaaa gcgcaacctg tggcgttcgg gcaacgcgcg cagccggttt
     5641 tctcgggacg aattgcctca tcagtggaag ctctaagccg tgatgccaat ggaacttgcg
     5701 ccccggcagt cggccaggcg gtccggcctt ggctcgccgg atgcttgcac tttctccgca
     5761 cgctgattct gatccacttg ttgctgcgga gcttatcgaa catgcggtct gtgccgctct
     5821 tgctcgtatt gccgttagtt tgctcggccg gaattggacg attgtctgcg gccgacggag
     5881 agctcgtggt gcgcgtggcc agcggacgcg agtttacagg gttgttcgac gatcgctccg
     5941 attctcagtc gctcgttctg cgctcgcagc gcgggagcgt gacgatccac cggccgatcg
     6001 agtgggaccg aatcgtgtcg gcgaccaaag atggccaggc ggtcacgatc gacgcgctcc
     6061 gccagagcat tgcgatgccc gtgccgcccg caccagcgag cgacgtgcgg acgatcatcg
     6121 tcgaaagccg ggagccggta gctggcgacg atgaagtcgt acaagcgacg gccctcgaac
     6181 cactgccgcc agtcacgacg atcaccttcg acgcccggat cgccaactgg gactgggatg
     6241 tcgaaacgga cggcgtggtg ctggacctgt tgccactcga tggcgacggc tatctcgcgc
     6301 cggcgagcgg caccgttcag gtggagctct acgcgccgca gcgccgccgt ttcgatctgg
     6361 ccccgcaaag cggcggcgac acgctggaac tggtcgaacg ctggacccgc gccatttacg
     6421 ccgcagattt cacgaccagc ggcgttcgcc tgcgcctgcc gtttggtgcg atccacccgg
     6481 aattcgattc ggattggctc gcctggcaat acggcctggt tcacgtgcga tttgccgcac
     6541 cgggccacgg cacattcgat tcctctcagg acggcatccg gatgcgtccc tgggcgccca
     6601 accgcgacca gctcgagctc aacacccgcc gccgcttctt gccaaccgaa gccacgggcc
     6661 ggccgaagga gtcgattggc ctgcacaagt agcgggtgca caccaacccg acgcgtaagc
     6721 gagggagtcc ctcacttacg taagcgagcg agaccctcac ttacgtttcg ggttaatatg
     6781 cccgccgcac ctggctgccg ccgacgcggc ggatcaggcg gcgcatctcc agggcgctga
     6841 tcgtcgccag cacattgtgg atcggcagcc cgctgctgac agcgacttcg tcgatgctga
     6901 tcgattcgtc gccgatgg
//
LOCUS       DASUGB010000667         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1324854, whole genome shotgun sequence.
ACCESSION   DASUGB010000667 DASUGB010000000
VERSION     DASUGB010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324854"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="VFV87_12760"
     CDS             complement(<1..1548)
                     /locus_tag="VFV87_12760"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX5104681.1"
                     /translation="MNLLTDSLVWCVLQITLLAVLALALCASVRRWSLVGNAALPAVA
                     LAAIVVLTVCAFLPWPAWWRFGPESKVAEANLAASNDSSPNSPSVDNAQTGATTVQTV
                     PAQTEPAAPAPQKERAGTLAPSESPTVFDPVAASPLAMPRWLGSALGVLLGIGVIAGL
                     VQIVGGLLSIRSYRRRSQRIDDAELNEQLDVLQAELGLVRCVELRECNCLTTAATCGW
                     RRPLVLLPPNWRQWSAAQRRAVLAHELAHVTSGDFLACILAQFGLALHFYHPLVHWLV
                     AHLRLEQELAADATAAALAGGRREYLTTLAELALHLQERSLGWPAHTFLPTPGTFLRR
                     IEMLRDSTPALATAPRRRWAVKWMAVGVLLLGAALIAGLRGGTTVSPFDRDTLAQDRP
                     DSAPGASPSSIDLKHVTNDARFVLIARPAQALAQEEIRTALASSGADASPVLKLLTDE
                     HLQQITLIGPAGLQLNELNDQVIAVLEFDRPMSLEDLGKTGALPQQIKPLNLGAANDT
                     DELAGCSL"
     gene            complement(1569..1982)
                     /locus_tag="VFV87_12765"
     CDS             complement(1569..1982)
                     /locus_tag="VFV87_12765"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5104682.1"
                     /translation="MARPRAKELTERELEVMRACWRKGEATATDIRDELAKDGRDLAY
                     TTVATLIRILCEKSFLAQTSQERPFTYKPIRTFDEVSGNLVWDLVERLFGGQREQLLV
                     RLLEERKLTAKERATLEEILRDAKQQDAKKPESKQ"
     gene            complement(2079..>2777)
                     /locus_tag="VFV87_12770"
     CDS             complement(2079..>2777)
                     /locus_tag="VFV87_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104683.1"
                     /translation="PPDQSVEPAEESHDEPAAAGAAAVGGSETKRETPAMGSGKGQAA
                     APGEIFVFLCPNGHKLNGPPSLKGKPGQCPHCGAKFRIPDDEDLELPEEEEVPMGEVD
                     EDEEPGQPSGSRSSFDFSQFMGGAAQDDAAVEAVVEEPPVAPPPPGPSGLGYIVGRLW
                     EQRTEGTELEIFLNEGEILAPDEFSEVLSSSDYGVFAIQEGDGTFAVSVVPWSAVRRI
                     GMRRMEDLPPQLFQ"
BASE COUNT          525 a          879 c          848 g          525 t
ORIGIN      
        1 gagcgaacag ccggcgagtt cgtccgtgtc gttggcagcg ccgaggttga gcggcttgat
       61 ttgctgaggc agtgcgccgg tcttgccaag gtcctcaagc gacatcggcc ggtcgaattc
      121 cagaaccgcg atgacctggt cgttcagctc attcaattgc aggccggcag gaccgatcaa
      181 cgtgatttgc tgcaaatgtt cgtccgtcag cagcttcagc acgggcgaag cgtcggcccc
      241 gctgctggcc agggcggttc tgatttcttc ctgggccagc gcttgagccg gccgggcaat
      301 caatacgaac ctggcatcgt tggtgacgtg tttcaggtcg attgaactgg gcgacgctcc
      361 tggcgccgaa tccggccgat cctgggccag agtgtcgcgg tcaaagggtg acacggtggt
      421 cccgccgcgc aggccggcga tcaacgcagc ccctaacagc agaacgccga ccgccatcca
      481 cttgaccgcc cagcgccggc gcggcgcggt ggccaaggca ggtgtggaat cccgaagcat
      541 ctcaattctc ctcagaaaag tgcctggggt cggcaaaaag gtatgggccg gccagcccaa
      601 cgatcgctcc tgcaagtgga gcgctagttc cgcgagagtg gtcaaatatt cccgccgccc
      661 gccggcaagc gcagccgccg tggcatcggc ggccaattcc tgctccagtc gcaggtgagc
      721 aacgagccaa tggacaagtg ggtgatagaa gtgcaatgcg agcccaaatt gggcgaggat
      781 gcaggcgagg aaatctccac tggtgacgtg cgccaactca tgggcgagca ccgctcgccg
      841 ctgtgcggcg gaccattggc gccagttggg cggcagcagg acaagcggcc gccgccagcc
      901 gcaggtggcg gccgtggtca ggcaattgca ttcgcgcagt tcgacacagc gaaccaggcc
      961 caactcggcc tgcaagacat cgagctgttc attaagttcg gcgtcatcga tgcgttggct
     1021 gcggcggcgg tatgagcgaa tcgacagcag tccgccgacg atctgcacca gtccggcgat
     1081 cactccgatc ccgagaagca cgcccagcgc cgagccgagc cagcgcggca tggcaagcgg
     1141 cgaagcagcg actggatcaa agactgtcgg cgattcggaa ggagcaagcg tcccagccct
     1201 ttccttctgc ggcgcgggag cggccggctc ggtctgcgcc gggacggtct ggacagtggt
     1261 tgcgccggtt tgtgcattgt ccaccgaggg tgaatttggc gatgaatcgt tgctcgctgc
     1321 aaggttggcc tccgcgactt ttgattccgg tccaaatcgc caccaggcgg gccacggcag
     1381 gaaggcgcag accgtgagga cgacgatagc cgccagcgca accgccggca gagccgcatt
     1441 gcctacgagc gaccagcggc gaacactcgc acataaggcc aaagccagca cggccagcag
     1501 cgtgatttgc agcacgcacc agacgagcga atcggtcagc agattcatgt tcgcctcgac
     1561 cggttgggtt attgcttcga ttcgggcttt ttcgcgtctt gttgtttggc gtcgcgcagg
     1621 atttcttcca aggtcgcccg ctctttcgcc gtgagcttgc gctcctccaa gagccgcacc
     1681 aacaattgct cgcgctggcc gccaaacagc cgctcgacca ggtcccaaac caaattgccc
     1741 gaaacctcat cgaacgtgcg gatcggctta taggtgaagg gccgttcctg gctcgtctgc
     1801 gcgagaaaac tcttttcgca aagaatgcga atgagcgtgg caacggtcgt ataggccaga
     1861 tcgcggccgt ccttggcgag ttcgtcgcgg atgtcagtcg ccgtagcttc gccctttcgc
     1921 caacaagcgc gcattacttc cagctcacgc tccgtcaatt ccttggctct gggtctggcc
     1981 atgttcagca cttgtgtttg gttatttaga tttggtttta tcgtagtcgc gtcggctcag
     2041 catgtcaagg aggaagaaat cgtggaatta tttgccagct actgaaagag ctgtggcggc
     2101 aaatcttcca tccgccgcat gccgattcgc cgtacagccg accagggaac gaccgagacg
     2161 gcgaacgtcc cgtccccttc ctggatggcg aacaccccat aatcgctgct ggaaagaact
     2221 tcggagaatt cgtcgggcgc cagaatctca ccttcgttta ggaagatctc cagttccgtc
     2281 ccttcggtcc gctgctccca caaacggccg acgatatagc ccagtccgct aggaccgggt
     2341 ggcggcgggg cgaccggcgg ttcttcgacc acggcctcaa ccgcggcatc atcttgagcc
     2401 gcgccaccca tgaactggct gaagtcaaag gagctgcgcg agcccgaggg ctgccccggc
     2461 tcttcgtcct catcgacctc gcccatagga acctcttctt cctcgggcag ttccaagtct
     2521 tcgtcatctg gaatacgaaa cttcgcccca cagtgcgggc actgccccgg cttacccttc
     2581 aggctgggag ggccattcag cttgtggccg ttggggcaca gaaatacgaa aatctcaccg
     2641 ggcgcagcag cctggccctt gccgctcccc atcgctggcg tttcgcgctt cgtctcgctc
     2701 cctcctacag ccgcagcccc ggcagccgct ggctcgtcgt gcgattcctc cgccggctca
     2761 accgactgat ccggcgg
//
LOCUS       DASUGB010000668         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1880028, whole genome shotgun sequence.
ACCESSION   DASUGB010000668 DASUGB010000000
VERSION     DASUGB010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880028"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1754
                     /locus_tag="VFV87_12775"
     CDS             30..1754
                     /locus_tag="VFV87_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104684.1"
                     /translation="MWTFFRKAIGGRSDNFRARRRLFFEPLEERRVLAGNVLAALDFA
                     GNLSLTGDGLDNDVTITFTADNTVDITGNAGTTIVGPATGVSVTGSIRASLLGGNDDL
                     TVVGGNFDLLGGGINYLGGTGDNDLTVSDLSLASNLLVQNSPNGVPPASPSETIVSNL
                     TATGVSVFNGANTSTVTTISGSTIAGSILISNPSGGSHSILLQNLLGVTSISATNGSA
                     TTSNSITLDGVDMNDAAIGGAILRNGNSGGSNFIDVRGAATDIRGSLSAANGFAGTTN
                     FTEIREGAVSGNMTLTNGNATTSAASRLGDIALVTVGGNLLATNGTSGSNSLRLDRAL
                     VAAGRSTTLLNGSSTVGENAIIIGAAPVTLGGGLIAMNGTSAANNRIEIEQATIGTAG
                     GAVDNVLLQNGNVGAGMISLVELGDVANVAIADGGLTIFDGAGNGLIDFIRATIAGGL
                     NIQAGAGNNTINLAVLAGATMSVAGTTNVSTQGGMDTLSIGTGGGIAVLDSIFASLGA
                     GNDILNIGAGAAGGSAFASTAGFLFDGGAGSDTANIEAAVNAALFGPPITPPAAARFL
                     NFETINVV"
     gene            complement(1798..2559)
                     /locus_tag="VFV87_12780"
     CDS             complement(1798..2559)
                     /locus_tag="VFV87_12780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5104685.1"
                     /translation="MTQTTPPSSGYRWNATPFAEGYDQAAEVIHPRYLEIQSVIASFL
                     PPLVAGDLVVDLGGGSGRLVERILDQFPKTSAIVLDQSEPFLALAERRLARFGQRATC
                     LLARLQDRWSTQLPARAAAIVSMSAIHHLEPAEKQSLYRQCFEALAPGGLLLNGDEVR
                     AAADDAYRAELWAWAGHMRAAMAGGTVPESFHPALEKWIDRNVVRFGEPKVSGDDCHE
                     TINAQLGYFRAAGFAFADCPWQQALWAVLRGQKAS"
     gene            2923..3330
                     /locus_tag="VFV87_12785"
     CDS             2923..3330
                     /locus_tag="VFV87_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5104686.1"
                     /translation="MAKRIYVGNLPWAYGSAELEQLFKQYGDVAAAEVIMDRETGRSR
                     GFGFVQMANDADMDAAINGLNGQDCNGRPLVVNEARERTPRSGGGGGGYGGGGGRGGG
                     GGGYGGGRGGGGGYGGGGGGYGGGGREGGGRRY"
     gene            3446..>3665
                     /locus_tag="VFV87_12790"
     CDS             3446..>3665
                     /locus_tag="VFV87_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104687.1"
                     /translation="MAGKLARRKRQKSDRAARNLADARSQAARTRGGRSPSVSAPAAA
                     IPDSSTTHKLIMGTAVVLPFLGCLAAIVL"
BASE COUNT          649 a         1143 c         1217 g          656 t
ORIGIN      
        1 aagatatgca gacaaaaagg agacctgcaa tgtggacttt tttccggaag gctattggtg
       61 ggcgttctga caatttccgc gcccgtcgca ggctgttctt cgagccgctt gaagaacgcc
      121 gcgttctcgc gggcaacgtg ctcgctgctc tggatttcgc ggggaatcta tccctcaccg
      181 gcgacggcct ggataacgac gtgacgatca cgttcacggc cgacaatacg gtcgatatta
      241 cgggcaatgc gggaacgacg atcgttggtc cggcgactgg tgtctcggtg acgggcagca
      301 tccgggcgtc gctgctgggc gggaacgacg atttgaccgt cgtgggtggc aactttgacc
      361 tgttgggcgg cgggatcaac tacctgggcg gcaccggcga caacgatttg acggtcagcg
      421 acctctcgct ggccagcaat ttgctcgtcc agaattcgcc aaatggggtc ccgcccgcgt
      481 cgcccagcga gacaatcgtc agcaacctga cagcgaccgg tgtgagcgtc ttcaatggcg
      541 cgaacaccag cacggtgacg acgatcagcg gctcgaccat tgccggcagc atcctgattt
      601 ccaaccccag cggcggcagc cattcgatcc tcctgcagaa tctgctaggt gtcacgtcga
      661 tttcggcgac gaacggctct gcgacaacga gcaacagcat cacgctcgac ggcgtcgata
      721 tgaatgacgc ggcaatcggc ggtgcgattt tacggaacgg caattcaggc ggctcgaact
      781 tcatcgatgt ccgcggagct gccaccgaca ttcgcggaag cctgtcggcc gctaacggct
      841 ttgccggcac gacgaacttc acggaaatcc gcgagggggc cgtttccggc aatatgacgc
      901 taaccaatgg caatgcaacg accagcgctg caagccgcct gggcgatatc gcactggtga
      961 ccgtcggcgg caatctgctg gcgaccaacg gcacatccgg atccaacagc ctgcggctcg
     1021 atcgggcgct cgttgcggcc ggacgcagca ctacgctcct caacggcagc agcaccgtgg
     1081 gcgaaaacgc gatcatcatc ggcgcggccc cggtgacgct cggcggtggc ttgattgcga
     1141 tgaatgggac gtcggcggcc aacaatcgca tcgagatcga gcaggctacg atcggcacgg
     1201 ccggcggggc ggtggacaac gtcctgctcc agaacggcaa cgtcggcgcc ggcatgatca
     1261 gcctggttga actcggcgat gtcgccaacg tcgccattgc cgatggcggg ctgacgatct
     1321 tcgacggagc cggcaacggt ctcatcgatt tcatccgcgc cacgatcgcc ggcggcctga
     1381 acattcaggc gggcgccggc aacaacacca tcaatctcgc cgtgctggca ggcgcgacga
     1441 tgagcgtcgc cggcacgacc aacgtttcga cgcaaggtgg catggacacg ctcagcatcg
     1501 gcaccggcgg cgggattgcg gtcttggaca gcatcttcgc atcgctgggc gcgggaaatg
     1561 acatcctgaa catcggcgcg ggcgcggctg gcggcagtgc gttcgcctcg acggcaggtt
     1621 tcctgttcga cggcggtgcg ggcagcgaca cggccaacat cgaagcggcc gtcaatgcgg
     1681 ccctgttcgg accaccgatc acgccccccg ccgcagcgcg gttcctgaat ttcgagacga
     1741 ttaacgtcgt gtgacgtcgt ggggcagaca ttcttgtctg ccagtgcgtt cgtcgcatca
     1801 actcgccttt tgcccccgca gcacggccca cagcgcctgc tgccaggggc aatcggcgaa
     1861 ggcgaagcca gcggcgcgga aatagccgag ctgtgcgttg atcgtttcgt ggcaatcgtc
     1921 gcccgagacc ttcggctcgc caaagcggac gacgttgcgg tcgatccact tctcgagagc
     1981 cggatggaag ctctcgggca ccgtgccgcc ggccatggcg gcgcgcatgt gcccggccca
     2041 ggcccacagc tcggcgcggt aggcatcgtc agcggcagcc cgcacttcat cgccgttcaa
     2101 gagcaacccg cccggcgcga gtgcttcgaa gcactggcgg tacagcgact gtttctcggc
     2161 aggttcgaga tgatggatgg ccgacatgct gacgatggcc gcggcccgcg ccggcaactg
     2221 ggttgaccac cggtcctgca agcgagctag caggcacgtc gcccgctggc cgaaccgcgc
     2281 cagtcgccgc tcggccaggg ccaggaacgg ctcggactga tcgagcacga tggcgctcgt
     2341 tttcgggaat tgatccagta tccgctccac cagccgcccc gatcctcccc ctaaatcgac
     2401 gaccagatcg ccggccacga gcggcggcaa aaacgacgcg atgacgctct ggatctccag
     2461 ataacgcggg tgaatgacct cggctgcctg gtcgtatccc tcggcaaaag gcgtggcatt
     2521 ccagcgatag ccggatgatg gaggagtggt ttgcgtcatg gcttgcaact tgctttgtag
     2581 gctggcgaaa gcaatgggcg gctggcagcc atgctctcta aacacgacgt taacagacgg
     2641 ctggcgagga aagtggttca gagccgccca ttcggtcttt cattacccgc tgctagcggg
     2701 tatagttctc ggcgtgaaca gttgccgacc tctggggagg ttggcggaac cgttgagctg
     2761 tgattgccct tgtccctctg ccgtttgccg accgggttgc ttccattcgt cgaacctagg
     2821 gtcctgtagg agcgcacgcg ccgcgcgccc cgccgctgcc ttgagctgcc gggcaactgt
     2881 ttggttttcc agtttcagtt tttggttcaa ggacctgtcg gaatggcaaa gcggatctat
     2941 gtgggcaatc tgccctgggc ttacggctct gcggagctcg agcaactgtt caagcagtat
     3001 ggcgatgtgg ccgctgcgga agtgattatg gatcgcgaga ccggtcgctc gcgagggttc
     3061 ggcttcgtgc agatggccaa cgatgcggac atggacgcgg cgatcaacgg tctgaatggg
     3121 caggattgca atggccgccc gctggtggtg aatgaagccc gcgagcgcac gccgcgttcc
     3181 ggcggtggcg gcggcggata tggcggcggc ggcggccgcg gcggcggcgg tggtggctat
     3241 ggcggcggcc gcggtggcgg tggcggctat ggcggcggcg gtggtggcta cggtggcggc
     3301 ggccgcgaag gcggcggcag gcgctactaa gccgcgacgc tttctcttgc cttgtaaact
     3361 atgaatgccg cccgcaccat tcgtgcggcg gcgctactgt ggccgcgcgc atgactccgc
     3421 gcggtttgct cgaaggggga cgttcgtggc tggcaagctt gcgcggcgca aacgccaaaa
     3481 gtccgatcgc gcggcgagga atctcgccga cgcgcggtcg caggcggcgc gcactcgcgg
     3541 agggcgatcg cccagcgtct cagccccagc cgcggcgatt cccgactctt cgaccacgca
     3601 caagctcatc atgggcaccg cggtggtcct ccccttcctc ggctgcctgg ccgccatcgt
     3661 gctgc
//
LOCUS       DASUGB010000669         6698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1880214, whole genome shotgun sequence.
ACCESSION   DASUGB010000669 DASUGB010000000
VERSION     DASUGB010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880214"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(816..1580)
                     /locus_tag="VFV87_12795"
     CDS             complement(816..1580)
                     /locus_tag="VFV87_12795"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104688.1"
                     /translation="MSESANRRRTTWSRSASPAPTWIEWLTILGILGVVGAFLWPMFA
                     VQREVDPPQRCQHLLKQIAVSLHAYHDNFGCFPPAYIADSTGRPMHSWRVLLLPYMEE
                     KPLYDQYRFDEPWDGPHNRRLASAIVRTYCGAADHAGPRSTETDYVAIVGPRTMWPEE
                     GSVAIGDLEDGPAQVVAVVEVHNSGIHWMEPRDLHANQMAPKINAPRGQGISSEHAGG
                     AYAATADGAVRFLKDDTPADVLRSWIQIDDGTLPLP"
     gene            complement(1593..2501)
                     /locus_tag="VFV87_12800"
     CDS             complement(1593..2501)
                     /locus_tag="VFV87_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5104689.1"
                     /translation="MTTSTPESPAETSPQPFQFRIRDLLLVMLLVGLAVGAVAQGSPE
                     LFLIFLLTGACCAYLWRELWMPRNPVPAIVTLGLIFLVLAGLMLPSVQTTRTPSVRGM
                     CGNNLLQIVLALENYHDVYGSFPPAYVADESGRPMHSWRVLLLPFIEHEDLYKQYRFD
                     EPWDGPNNRQLARSISRVYACPADHRDGRPETSYVAIIGPHTAWPGDRPVRRADMKDG
                     PSNVLLVFEVKDSGIHWMEPRDLHVNQMAPAINAPHGQGVSSKHPGCAQAAFADGRVR
                     QLPDNLPPSYLRSWMQIDDDTLGEPP"
     gene            2714..2890
                     /locus_tag="VFV87_12805"
     CDS             2714..2890
                     /locus_tag="VFV87_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104690.1"
                     /translation="MRCRLHTLLIVLAVAPPVLAGLWSVVSDPWPVPLTELTVFAAIA
                     AAMTGAAVLGAAAQ"
     gene            2909..3202
                     /locus_tag="VFV87_12810"
     CDS             2909..3202
                     /locus_tag="VFV87_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104691.1"
                     /translation="MFRFSLRDLLWLTLVAAILCAWWIQWRQHVLTEQRDAETIQTLA
                     LQAQQNANVRIIPIGTPPGIRRPRTPGSRSPAVDEWDLQAPPEILQRIDRPEP"
     gene            3218..3379
                     /locus_tag="VFV87_12815"
     CDS             3218..3379
                     /locus_tag="VFV87_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104692.1"
                     /translation="MRYRLRTLLIVLTVAPPILAAAWWAYASGRVRWQNWAIVIDQSP
                     PTQFEKESP"
     gene            3376..3996
                     /locus_tag="VFV87_12820"
     CDS             3376..3996
                     /locus_tag="VFV87_12820"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104693.1"
                     /translation="MTRTTRYIVALLLGVSLNPAALAAGELSEEEKAAGFVPLFNGTD
                     LTGWRFTGSDESPPKVLPENWKVEEGVIKVTGGGKPHLATAEKFADFELRLEWRGMRD
                     KYNSGLFIRSKPDLGANQLNLARGSEGAFINGKVEGARPAGDLQKAPGEWNEWRIVAV
                     GEKLTFYCNGQLAWEATGFKPAEGHIGLQAEGAPLEFRNIRLRKIE"
     gene            complement(3999..6365)
                     /locus_tag="VFV87_12825"
     CDS             complement(3999..6365)
                     /locus_tag="VFV87_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104694.1"
                     /translation="MPAQPQVAPLGPLVAEQIDLDPSITETPALPVSLPILNTAPALG
                     SPAGPLELEPLTSSSQPLIPQLPQEEPRDVELPSEEPQNLEQRNFAPEPEPQPEYPVT
                     KAWAYPAGLIEQLNELAATVPVAADWAERTKAEIDQFVSAQTLADGQTAQSLANLQRL
                     ADEARELALAQPEESEARSKLLRAGYAIVRRLAVWEQVHAIAVKGETVSAPVLDREAW
                     LAALSHIDSQLQATGAAANWREYLLIDRARQEFDSSSHPPADQRKLAQDILHRMHSTQ
                     LSEDQQAFLKSPPFAALDEQLRVRADELPDLIGLVAAIEEHEHLDTSLHARSVGGQYQ
                     ALRWSYDPAVAELADTVNTYYRNANIRVALSVELVNRLIPQQQAQVEPVRDNILGADV
                     EGRSHTRTRLRMVLLPDRHRWQIGLEAEGYVNSDTASSKGPATFYQTGDASFRARKRL
                     TVDRRGIRLFNAEANAASHTYLKEFETDFDGIPLLSNVARVVARNQYDSSQYDAKVEV
                     EGRIVGRATSQLDRQVAERLQKAKQDVQAKLVTPLQKLNLEPTAVDLETTADRLIARY
                     RVASAEQISAHTPRPQAPGDSLLSVQVHESMLNNVLEKLNLHGRRIELRELFKEITTR
                     FNREPIPIPEDLPEEVYVTFADEDPVRVDCQEGRVRLTIRLKELDSGRNQWHDFTVRG
                     YYVPNPDQRDANLVRDGVVELIGERLRFGDQVALRAIFARVLSRNRKLNLINKQIAQA
                     PELSDQQVTQFVIHDGWIGIALGPQTPGRQVAMYPRPESESEDDTRNE"
BASE COUNT         1132 a         2234 c         2103 g         1229 t
ORIGIN      
        1 ggatcaggta ttgcccgatc gacaatcccc ccgctgggcg cggcgctgtc attccagcgg
       61 cgggttgcgc ggcctttccg gcgtccgcca gcatcttgac cagttgcgac atccgcgtgc
      121 tcctcatggc tgggcctgcc ctgcaggcag gctgcggtcc tttgctgcat gccaagttta
      181 gcgctgggct ggctttttgc gcaggccaaa aagcgctaaa ccattcccca actgcttgcc
      241 gctcaacatc ttgcgacggc taaacggcgc gccccgccgc gaggcagtct agcaggttta
      301 gggcgggcca atgacccgcc atcacgctgc gagcactcgc gccgctgctg cgcaactttt
      361 cacacttttt ttgcccgccg cgccccgctc gacatcgtcc taactcctat gctgggaaaa
      421 acttgcgtag cgcaacaccc cccgcatgag gatgttgcgc tgttgcgcgt tgtcaagcac
      481 ctttcctccg cttcttggtg gccgccgatc tgtgtccctg ccaggccctg agaaccagca
      541 agccgctcga ttgaatcaat tgtacacatt acgccgccgc cgggcaaccc accttcctgg
      601 tagtggacaa attgtccagt tcgtcaagga ccgcccgcac ctcttcgacc cagagtatct
      661 tgcccgctcc aggcctgtct ccttgggcac gcctccaaga acggccgcga gcagcccggc
      721 ggtcgtctga cgccggaaca tacaagcgga acccgtattc aacgcagcca actcagtcct
      781 gcgaacgact gccgatgccc gccggtctgg gttccttacg gcagcggcag cgtgccgtcg
      841 tcgatctgaa tccagctccg cagcacatcc gccggcgtat cgtccttgag gaaccggacc
      901 gctccatccg cggtggccgc atacgctccg cctgcatgct cgctggagat tccctgcccg
      961 cggggcgcat tgatcttcgg cgccatctgg ttcgcgtgca aatcccgcgg ctccatccag
     1021 tgaatgccgc tgttgtggac ttcgacgacg gcgaccactt gggccggccc gtcctccagg
     1081 tcgccgatcg ccaccgaccc ctcttcgggc cacatcgtcc ttggcccaac gatcgccaca
     1141 tagtcggtct ctgtgcttcg cggccccgca tgatccgccg cgccgcaata cgtcctcacg
     1201 atcgcactcg ccagccggcg gttgtgcggg ccatcccacg gctcgtcgaa gcggtactgg
     1261 tcgtagagcg gcttctcttc catgtagggg aggagcaaca cccgccaact gtgcatcggc
     1321 cggcccgttg aatcggcaat gtacgccggc ggaaaacagc cgaagttgtc gtgataggcg
     1381 tgcagagaca ccgcgatctg cttgagcagg tgctggcagc gctgcggcgg atcgacttcg
     1441 cgctgtacgg cgaacatggg ccacaggaac gccccgacga cacccaaaat gcccaggatc
     1501 gtcagccact cgatccatgt cggagcaggg ctggcgctgc gcgaccatgt cgttctgcgg
     1561 cgattggccg attcgctcat gggcggtcgc ccttacggtg gttcgcccag cgtgtcgtcg
     1621 tcaatctgca tccagcttcg caggtagctg ggcgggagat tgtcgggcag ttggcgaacc
     1681 ctgccgtccg cgaaggcggc ctgagcgcag ccaggatgct tgctggaaac gccctgccca
     1741 tgcggagcat tgatcgccgg cgccatctgg ttcacgtgca aatcccgcgg ctccatccag
     1801 tggatgccgc tgtctttgac ttcgaaaacg agcagaacgt tggatgggcc gtccttcatg
     1861 tcggcccgtc gcacggggcg atcccctggc caggccgtgt gcggaccgat gatggcgaca
     1921 tagctggttt ccggtcttcc gtcgcgatgg tcagcaggac aagcatagac ccgcgaaatc
     1981 gacctggcga gttggcgatt gttcggccca tcccaaggct cgtcgaaccg gtactgcttg
     2041 tagagatctt cgtgctcgat aaatggcaac agcaacaccc gccaggaatg catcgggcgg
     2101 ccgctctcgt cggcgacgta tgccggtggg aaggagccgt acacgtcgtg gtaattctcc
     2161 agagcaagca caatctggag caggttgttg ccgcacatcc ctcggacact cggagtgcgt
     2221 gtcgtctgta cggatggcaa catcaggccg gccagaacca ggaagatcag ccccagggtc
     2281 acgatggccg gaaccggatt tctcggcatc cacaactccc gccacaggta ggcgcagcat
     2341 gcgccagtca gcaggaagat caggaacagc tcgggacttc cttgggcgac agcacccaca
     2401 gcaagcccca ccagcagcat caccaacagc aaatcccgaa tccgaaactg aaacggctgc
     2461 ggactggttt cggctggcga ttcgggcgtg ctggtcgtca tggcgaatct ccgcaccgcc
     2521 gcgaatgcgg cttcaaagca tgtcgtgcga tcgcgccctc ccattccgcc tccagcttag
     2581 cccccgccgc tccgaattgc acgccgcaag tccaggctca tccgtgttcg attcacgttc
     2641 cctccgtggc ttccctccgg cagcgggtcc aacgatggcg ctggatcgca tccgctcggg
     2701 tagaatcgcc aggatgcgtt gccgtctcca caccctgttg atcgtgctgg cggtcgcccc
     2761 gccggtcctc gccgggctgt ggtcggtcgt gagcgatccc tggccggtgc cgctgacgga
     2821 gttgaccgta ttcgccgcga tcgccgccgc gatgaccggc gccgccgtcc tcggcgccgc
     2881 tgcccaatag ccgaacaggt gccgagccat gttccgcttc tccctccgcg acttgctgtg
     2941 gctgacgctt gtggcggcga tcctctgcgc gtggtggatc cagtggcggc agcacgtgct
     3001 aacggaacag cgcgacgccg agacgattca aaccctcgcc ctacaggctc agcagaacgc
     3061 caacgtccgg atcattccca tcggcacgcc accaggaatt cgccggccgc gcacgccggg
     3121 cagccgaagt cccgcggtgg atgaatggga cctgcaagcc ccgccggaga ttctccagag
     3181 aatcgatcgg ccggaaccct gaaatccggc aaacgtgatg cgctatcgcc tccgcacctt
     3241 gctgatcgtg ctcaccgtcg ccccgccaat cctggccgcg gcgtggtggg cctatgcttc
     3301 cggccgcgtg cgctggcaga attgggccat cgtcatcgac cagtcaccgc ccacccagtt
     3361 cgaaaaggag agtccatgac tcgcacgact cgctacatcg ttgcactgct cctaggcgtc
     3421 agcctcaacc cagcggcttt ggcagccggc gagctctccg aggaagaaaa agccgccggc
     3481 ttcgtgccgc tgttcaatgg cacggacctg accggctggc gattcacggg gagcgacgaa
     3541 tcgccgccca aggtcctgcc ggaaaactgg aaagtcgaag aaggcgtgat caaagtcacc
     3601 ggcggcggca agccgcacct ggcgacggcc gagaaattcg ccgatttcga gctgcgcttg
     3661 gaatggcgcg ggatgcggga caagtacaac agcggcctct ttatccgcag caagccggac
     3721 ctcggtgcga accagctcaa cctggcccgc ggcagcgagg gcgccttcat caacgggaag
     3781 gtcgaagggg ccaggcccgc gggcgacttg cagaaagccc ccggcgagtg gaacgagtgg
     3841 cggatcgtcg ccgtcggcga aaagctcacg ttctattgca acgggcagct ggcctgggaa
     3901 gccaccggct tcaagcccgc cgaggggcac atcggcctgc aagccgaggg cgcgccgctg
     3961 gagttccgca atatccggct gcggaagatt gagtaggctt actcgttgcg cgtgtcgtct
     4021 tcgctctcgc tctccggccg cgggtacatc gccacttgcc ggccgggagt ctgcggcccc
     4081 agggcaattc cgatccagcc gtcgtgaatg acgaactggg tcacttgctg gtcggatagc
     4141 tcgggggcct gggcaatctg cttgttgatc aggttcagct tgcggttcct cgaaaggacg
     4201 cgggcgaaga ttgcacgcaa ggcgacctgg tcgccgaacc gcagccgttc gccgatcaat
     4261 tcgacgacgc cgtcgcgcac cagattggcg tcgcgctggt cggggttggg cacgtagtag
     4321 ccgcggacgg tgaagtcgtg ccactgattg cggccgctgt ccagctcctt caagcgaatc
     4381 gtgagccgca cgcgcccctc ctggcaatcc acccgcacgg ggtcctcgtc ggcgaaggtc
     4441 acatagacct cctcgggcag gtcttcggga attggaatcg gctcgcgatt gaagcgggtg
     4501 gtgatttctt tgaacagctc gcggagctcg attcgacggc cgtggagatt cagcttctcc
     4561 agcacgttgt tgagcatcga ttcgtgaacc tgcacgctca gcaggctgtc gccgggggcc
     4621 tgcggcctag gcgtatgggc cgaaatctgc tcggcggaag ccacgcggta gcgggcgatc
     4681 aagcggtcgg cggtcgtctc cagatcgacg gcggtcggct ccaggttgag cttctgcagc
     4741 ggcgtgacca acttggcttg cacgtcctgc ttggcctttt gcagtcgttc ggcgacctgc
     4801 cggtcgagct ggctggtcgc ccggccgacg atccttccct cgacctcgac tttcgcgtcg
     4861 tactggctgc tgtcgtattg attgcgagcc acgacccgcg cgacattgct caacagcgga
     4921 atgccgtcga aatcggtttc gaactctttc aggtaggtgt gcgacgcggc gttcgcttcg
     4981 gcattgaaca ggcgaatgcc ccggcgatcg accgtcagcc gcttacgggc ccggaacgac
     5041 gcatcccccg tctggtagaa cgtggccggc cccttcgacg aagccgtatc cgaattcacg
     5101 tagccctcgg cttccaggcc gatctgccag cggtggcggt cgggcaacaa caccatccgc
     5161 aggcgcgtgc gggtgtggct gcggccttcg acatcggccc caaggatatt gtcccgcacc
     5221 ggctcgacct gggcttgctg ctgcggaatc aggcggttga ccagctcgac ggacagggcc
     5281 acgcgtatgt tcgcattgcg gtaatacgtg ttgaccgtgt cagccagctc ggcgacggcc
     5341 ggatcgtacg accagcgaag ggcctggtat tgcccaccga ccgagcgggc gtgaagcgaa
     5401 gtatcgaggt gttcgtgctc ctcgatggcc gcgaccaggc cgatcaagtc cggcagctca
     5461 tcggcccgca cgcgaagctg ctcgtccagc gccgcgaacg gaggcgattt caggaaggct
     5521 tgctgatctt cggaaagctg cgtgctgtgc atgcggtgca gaatatcctg cgccagtttg
     5581 cgctggtcgg ccggcggatg gctggacgag tcgaactctt gtcgggcgcg gtcgatcagc
     5641 agatattccc gccagttcgc cgcggcgccg gtcgcctgga gctgcgaatc gatgtgtgac
     5701 agcgccgcca gccacgcctc gcggtcgagc accggcgcgc tcacggtctc gcctttcacg
     5761 gcaatcgcat ggacctgttc ccagaccgcc aatcgccgca cgatcgcata tcccgcccgc
     5821 aacagcttcg agcgggcctc gctttcctcc ggctgcgcaa gcgcgagctc cctggcctcg
     5881 tctgccagcc gctgcaggtt cgccagagac tgggccgttt gcccgtcggc cagcgtctgg
     5941 gcactcacga actggtcgat ctcggccttg gtccgctcgg cccagtcggc ggcgacggga
     6001 accgtggccg cgagttcgtt cagctgctcg atgagccctg ccggataggc ccaggccttc
     6061 gtgacggggt attcgggctg cggctcgggt tcgggcgcga agttccgctg ctccaaattc
     6121 tgcggctcct cgctgggcag ctccacgtcg cgcggctctt cctgcgggag ctgcggaatc
     6181 agcggctggc tactggacgt gagcggctcc aattccagcg gaccggcggg gctgcccagc
     6241 gcgggcgcgg tgttcagtat cggcaggctg acggggaggg ccggcgtctc ggtgatcgag
     6301 ggatcgagat cgatttgttc ggccaccaag gggcccagcg gcgcgacctg cggttgggcg
     6361 ggcacgtcgg ctgtctcgct ggtctcgctc ctggcaagcg agggccgccc gttggactgt
     6421 gaagcaagtc cgtgcctctt cccgggctcg cgggaatgct gccagaggta gggaactatc
     6481 agtcccagtc cagccaaccc cgcgagcagc accagccaac cccacaatgg cgatcgatcg
     6541 tccgcacgcc tcatccggct ttcctccttg aaagcgggcc gctcctgcta gcgggcgcgg
     6601 catccgtcca tcgagaaaca tcggacggcg aaagcgcgcg catcgaacgc tcggcggaca
     6661 atctggccaa ccggcccatc cgctgcagct tgtcagcc
//
LOCUS       DASUGB010000670         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_74409,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000670 DASUGB010000000
VERSION     DASUGB010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74409"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFV87_12830"
     CDS             <1..444
                     /locus_tag="VFV87_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104695.1"
                     /translation="APAGLIQDQLTVVTNDTKLPTVSLPVEGRVVPALAVSPSPLFFG
                     RLQPGQTATKQLVITGKQPFAITAIHAPGLAPLEFRTSPDVTKKVHLVPVILTASDQP
                     GSFDYEVRIETDLPGSATTTCRVRGSVAGSSADDESEQLSGLPRR"
     gene            complement(458..1861)
                     /locus_tag="VFV87_12835"
     CDS             complement(458..1861)
                     /locus_tag="VFV87_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5104696.1"
                     /translation="MADDLASKSADDTWREGLSSPESHSEGHAAASGEGAHPPLHIGT
                     APEHQPVHESLAVEPVAAALAALHPLDRPDRQERSSRSETAEANRIRLAWAKLLWLLS
                     FLAVLLAISYLVPYIAEQTQYAITRGRQRAEHDYAREHLEGSPLGQLSRAYQMVSQAV
                     GPSVVHISTQGSGGTDMYTRPAFGSFPRTPSDGQGSGCIVDASGYILTNYHVVRDARE
                     IKVSLADGRRVPAQMVGFDPLTDLAVVKVEADKLTAAEWGDSDDAQVGALVWAVGSPF
                     GLERTITSGILSAKHRAGLAGTPYQDFLQSDAAVNPGNSGGPLVNSDGRVIGINTAIV
                     GDAYQGVSFAIPSSVAREVFQRIRSEGLVRRGWLGVRLDLVDDTRAQEIGLPQKTGVY
                     IVEVVEQEQGGSPAAKAGILPGDVILRWNDEEVASPELLSTLVAKANIGSRAKIVLFR
                     DGREVEVEATIGQRMID"
     gene            1978..2646
                     /gene="aat"
                     /locus_tag="VFV87_12840"
     CDS             1978..2646
                     /gene="aat"
                     /locus_tag="VFV87_12840"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5104697.1"
                     /translation="MLNPRERPRFPPVEAAGPDGVLFVGGSLAPDWMLEAYRRGIFPM
                     PIKVERRRHIAWLSPDPRGILEFDRLHISRRLVRRLKRGEFQFTFNRAFAKVVEGCAA
                     PRDADDDCWLTLAMQNAYLNLHELGHAHSVEVWQAGGLVGGVFGVAIGGVFAGESMFH
                     RVTDASKAALAVLVEYLRQRGYSLLDVQWTNAHTRRLGAIDIPRSEYLRQLSEAVERR
                     VTFS"
     gene            complement(2718..>3098)
                     /locus_tag="VFV87_12845"
     CDS             complement(2718..>3098)
                     /locus_tag="VFV87_12845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5104698.1"
                     /translation="ADKAPKTVKNFEDLVGKGFYNGLKFHRVIPDFMVQTGCPQGTGT
                     GGPGYKFADEFHKDLKHSGPGVLSMANSGPNTNGSQFFITHVATPWLDGKHSVFGQVL
                     EGQDVVNAIQQGDVMKSVSVAEAE"
BASE COUNT          507 a         1034 c          970 g          587 t
ORIGIN      
        1 gcgcccgccg ggctgattca ggaccagctc accgtcgtta ccaacgacac caagctgccg
       61 acggtgtcgc tgccggtgga aggtcgcgtt gtgccggcgc tggcggtcag cccgtccccg
      121 ctcttcttcg ggcgattgca gcccgggcaa acggccacca agcagctcgt catcaccggc
      181 aagcagccgt ttgccattac ggcaattcac gctccgggcc tggcgcctct cgaatttcgc
      241 acctctcccg atgttacgaa gaaggtccac cttgtccctg taattctcac cgccagcgat
      301 cagccgggca gcttcgatta cgaggtgcgg atcgaaaccg atctgccggg cagtgcgacg
      361 acgacctgcc gcgtgcgcgg gtcggtcgcc ggctcctcgg cggatgacga gagtgagcag
      421 ctcagcggac tgccgcggcg gtgattgacg cgcagcgtca gtcaatcatc cgttggccga
      481 tggtggcttc gacttcgact tcgcgcccgt cgcgaaacag gacgatcttc gcccgcgagc
      541 cgatgttcgc cttggctaca agcgtgctga gcaattcggg gcttgcgacc tcttcgtcgt
      601 tccaccgcag aatcacgtct cccgggagaa taccggcctt ggcagccggc gagcccccct
      661 gctcctgctc gacaacttcg acgatataga cgcctgtctt ctgcggcaag ccgatctcct
      721 gggcgcgcgt gtcatcgacc aggtccaacc gcacgcccag ccacccgcgg cgaaccaggc
      781 cctccgatcg aatccgctgg aaaacctcgc gggcgacgct gctggggatg gcgaagctga
      841 ctccctgata ggcgtcgccc acgatcgccg tgttgatgcc gatcacccgc ccgtcgctgt
      901 tcaccagcgg tccgccgctg ttgcccggat tgaccgcggc gtcgctttgc aggaaatcct
      961 ggtagggtgt gcccgccagg ccggcgcggt gcttggcgct gagtatgccc gacgtgatcg
     1021 tccgctccaa gccaaacggg ctgccgaccg cccagaccag cgccccaacc tgggcgtcgt
     1081 cgctgtcgcc ccactcggca gccgtcagtt tgtccgcttc gaccttcacc acggccagat
     1141 ccgtcagcgg atcgaagccg accatttggg cgggcacgcg gcgaccgtcg gcgagagaaa
     1201 ctttgatttc ccgcgcgtcg cggacgacat gatagttggt caggatgtag ccgctcgcat
     1261 cgacaataca cccggagccc tggccgtcgg agggtgtccg gggaaaggaa ccgaaagccg
     1321 ggcgggtgta catatcggtc ccgccgctgc cttgggtgct gatgtggacg acgctcgggc
     1381 ccactgcctg cgagaccatt tgataggccc gcgaaagctg gcccagcggt gaaccctcca
     1441 agtgctcgcg ggcgtagtcg tgctcggccc gctgacggcc gcgggtgatc gcgtattgcg
     1501 tctgctcggc gatgtacggc accagatagc tgatcgccag cagcactgcc aggaacgaca
     1561 aaagccagag gagcttcgcc cacgccagcc gaatccggtt ggcttcggcc gtttcgctcc
     1621 gcgagcttct ctcttgccga tcgggccggt cgagcgggtg cagcgcggcc agcgccgccg
     1681 ccacgggctc tacagccagc gactcgtgaa ctggctgatg ttccggcgcc gttccaatgt
     1741 gcagtggcgg atgcgctccc tcgccgctgg cggcagcatg tccctcggaa tggctctccg
     1801 gcgacgaaag cccctcgcgc caagtgtcgt ccgccgattt ggaagcgaga tcgtctgcca
     1861 ttgcagcaac cgatggttca gaaatcaggc tcaattatac cccttcatcc gcgcccgtag
     1921 gagtgatttt cccgggccaa tgatttgcca cccgccgaca ggttatctta aaggaggatg
     1981 ctcaaccccc gcgaaaggcc ccggtttccg cctgtggagg ctgccggtcc ggacggcgtg
     2041 ctcttcgtgg gcgggtcgct cgcgccggat tggatgctgg aggcctatcg ccgcggcatc
     2101 ttcccgatgc cgatcaaggt cgagcggcgg cggcacatcg cctggctctc ccccgatccg
     2161 cggggaatcc tggaattcga ccgtctgcac atttcgcggc ggctggtgcg gcggctgaag
     2221 cggggcgagt tccagttcac cttcaaccgg gcctttgcga aggtcgtcga gggctgcgcg
     2281 gccccgcgcg atgccgacga cgattgctgg ctcacactgg ccatgcaaaa cgcctatctc
     2341 aacctgcacg agctgggcca cgctcacagc gtcgaagtct ggcaagccgg ggggctggtc
     2401 ggcggcgtct tcggcgtggc gattggcggc gtgtttgccg gcgaatcgat gtttcaccgc
     2461 gtcaccgacg cctcgaaagc cgccctggcc gttctcgtcg aatacctccg ccagcgtggc
     2521 tactcgctgc tcgacgtgca gtggaccaat gcccacacgc gacgcctggg cgcgatcgac
     2581 attccgcgga gcgagtacct gcgccagctt tccgaggcgg tggaacggcg cgtgacgttt
     2641 tcgtagttca aggttcaaag ttcaatgttc aaggttgcat gcaagaaact ttgaacttgg
     2701 aacattgaac attgaactca ttccgcctcc gccacgctca cgctcttcat cacatccccc
     2761 tgctgaatgg cgttgacgac gtcttgcccc tcgagcacct ggccgaacac cgagtgcttg
     2821 ccgtcgagcc agggcgtggc cacgtgcgtg atgaagaatt gcgagccgtt ggtgttcggg
     2881 ccgctgttgg ccatcgacag cacgcctggc ccgctgtgct tcaggtcctt gtggaattcg
     2941 tcggcgaact tataccccgg cccgccggtc cccgttccct gcgggcagcc ggtctgaacc
     3001 atgaagtccg ggatgacgcg gtggaacttg aggccgttgt agaagccctt gcccaccagg
     3061 tcctcaaagt tcttgacggt cttcggggct ttgtcggc
//
LOCUS       DASUGB010000671         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_493241, whole genome shotgun sequence.
ACCESSION   DASUGB010000671 DASUGB010000000
VERSION     DASUGB010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493241"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFV87_12850"
     CDS             complement(<1..990)
                     /locus_tag="VFV87_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104699.1"
                     /translation="MLLAAPKLRAQEADQPLREIFVPFEDLNVILESDKQRVFLTRQE
                     YDELIQQAKSKPQTPAPHKVVLTSAEYSGQLEEGRATIRGTLTIDVLDGGLYALPLEL
                     GGVGIRSATLDGKPAPLSKNESNQPIVLIQGTGQHKLDLQLMAPLQTAAAQQTLNVAL
                     PSHAATRLKLAVPGNIDVKSGGAVISRTYEMEANRTLLEVLPQRGWLTLVMSLNNRLL
                     QDQRVFVARSVIVDEVTQGYERIHATVSFRILHGAVDKLRLSVPAGFEVTRVESPLLA
                     RWEEKADAAGAKTLEATLREPTSEQVVLQITANRSPVADADWLAGLQDWKFPQL"
     gene            complement(1136..>2190)
                     /locus_tag="VFV87_12855"
     CDS             complement(1136..>2190)
                     /locus_tag="VFV87_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104700.1"
                     /translation="RTSIDRAFVRMVVTRGDQIAVQALFRLRSARQRVAIIIPGIDPG
                     DGEQVARALDSQALRINNQVVGLESDNKTQFFIPITGHSPDEELLVELRYSVPGNQSQ
                     LDLPEFPEDPAVQQVQLAAYLPEEQKLLAIGGPWTDESSTTWIDRPFRDEGPDDETIL
                     SQLRSGIDCGSAGEGFPVDGDRYLFSALRPAPAPKGSLKLTSLHRNAVYAGVFLLVAA
                     IGLALTPRPAGQRLWFLAGLIVLVVLIAVFSPTLAQAVLGWPLYLAIGLVLLVWTVRC
                     LAWCIPGCVNCCSAYFKQAATAATVAAAASTSPAASATPPPTPEPAAGETPFAPQTPP
                     VAPPSDERKEGGESHG"
BASE COUNT          369 a          718 c          727 g          376 t
ORIGIN      
        1 aagctgcggg aatttccagt cctgcaggcc cgccagccaa tcggcgtcgg caacgggcga
       61 gcggttggcc gtgatttgca gcacgacttg ctcgctggtc ggctcgcgga gcgtggcttc
      121 cagggtcttg gccccggcgg cgtcggcctt ctcttcccag cgggcgagga gcggcgattc
      181 gacccgcgtg acctcgaacc cggcgggcac gctcagacgc agcttatcga ccgctccgtg
      241 caggatgcgg aacgagaccg tcgcgtgaat ccgctcgtag ccctgcgtga cttcatcgac
      301 gatcacgctg cgagccacga acacccgctg atcctgcagc agccggttgt tgagcgacat
      361 caccagcgtc agccagcccc gctgcggcag aacctccagc agcgtgcggt tggcctccat
      421 ctcataggtg cggctgatca ccgcgccgcc gcttttgacg tcgatgttgc cggggacggc
      481 gagtttgagt cgggttgccg catggctcgg cagagcgacg ttgagcgtct gctgagccgc
      541 cgccgtctgc aggggagcca ttagttgcaa gtccagcttg tgctgccctg tgccttggat
      601 cagcacgatc ggttggttgc tttcgttttt cgagagtggg gcgggcttgc catccaacgt
      661 tgccgagcgg atgccgacgc ctcccagttc cagcgggagg gcatagagcc cgccatcgag
      721 cacgtcgatc gtcagcgtgc cccggatggt cgcccgccct tcctcgagct gtccgctgta
      781 ttcagccgat gtgagcacaa ccttgtgcgg agcgggcgtc tgcggcttgg atttcgcctg
      841 ctggatcagc tcgtcatatt cctgccgcgt gaggaacacg cgctgcttgt cgctttcgag
      901 gatgacgttc aggtcctcga acgggacgaa gatttcgcgg agcggctggt cggcctcttg
      961 agcgcgcaat ttcggcgcgg cgagcagcat caaagctgcg aacgcggcga gccaaagtgg
     1021 tccgccgctc cgcggcagga ccgatgcgcg acgggatcta ccattgggga atccgtgagg
     1081 cgatcgaagc tcaccagcac cgctggcctg ccgcggagcg gcggcccaca tttgactaac
     1141 catgactctc ccctccttcc ttccgctcgt cactcggcgg agcgacgggg ggcgtttggg
     1201 gggcgaacgg cgtttcgccg gctgccggtt cgggggtcgg cggcggagtg gcgctggccg
     1261 cgggggaagt gctcgcggcg gcggcgacgg tcgcggcggt cgccgcctgc ttgaaatacg
     1321 ctgagcagca attcacgcag cccggaatgc accacgccag gcaacggacc gtccacacca
     1381 ggagcacgag gccaatcgcc agatacagcg gccagccgag cacagcctga gccagcgtcg
     1441 gcgaaaaaac ggcgatcagc acgaccagca cgattaatcc agccaggaac cacagccgct
     1501 gtccagcagg ccgcggcgtg agcgccagcc cgatcgccgc caccagcagg aacacgcccg
     1561 catacacggc gttgcggtgc aggctcgtca gcttcagcga ccccttcggc gcgggagccg
     1621 gccgcagggc ggagaacaga taccgatcgc catcgacggg gaagccttcg cccgccgaac
     1681 cacagtcgat tcccgaacga agctggctca ggatcgtctc gtcgtccggg ccttcgtcgc
     1741 ggaacggccg gtcgatccag gtcgtggaag attcatccgt ccacggcccg ccgatcgcga
     1801 gcagcttctg ctcttcgggc agatacgccg ccagttgcac ctgctggacg gcggggtctt
     1861 cgggaaactc ggggagatcg agctgcgact gattgcccgg caccgagtag cgcagctcga
     1921 cgagcagctc ctcatcaggc gaatggccgg tgatcgggat aaagaactgc gtcttgttgt
     1981 cgctttccag tccaacgacc tgattgttga tccgcagggc ctgtgaatcg agcgcgcggg
     2041 ccacctgctc tccgtcgccc ggatcaatgc ccggaatgat gatcgcgacc cgctgccggg
     2101 ccgatcgcaa ccggaacagg gcctgcacgg cgatctggtc gccgcgggta acgaccatcc
     2161 gcacgaaggc ccggtcgata ctcgttcgtt
//
LOCUS       DASUGB010000672         5160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_909265, whole genome shotgun sequence.
ACCESSION   DASUGB010000672 DASUGB010000000
VERSION     DASUGB010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909265"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1018)
                     /locus_tag="VFV87_12860"
     CDS             complement(59..1018)
                     /locus_tag="VFV87_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104701.1"
                     /translation="MSQPSENRPTYPPFDPPPGQPTPVPPPAPGPVGPFPDQAPKRPR
                     WGPRLIVVVLIALLVAPCAAVLAPREIAHWYLASAQELRSSGQKDEAYNRLAEAIRWS
                     PKHAGLLLQRAAWRLEDSQAEKALEDANQAAELARDNFTVLYLRSQILQQLDRHAEAI
                     QDWKAIDRLSLTRGTPSRAMALNGLAYARAVGRLELDEALKNVAEALELEPKSAAILD
                     TRGYLRHLNGENEKAIVDMDAAVKGVEEEFSAIDNPRPERGRSVLSSDIDLTGLNKPE
                     QGVAVIHYHRALVLKTLGREKDAKKDLQRARELIGHEPDETLF"
     gene            1293..2312
                     /locus_tag="VFV87_12865"
     CDS             1293..2312
                     /locus_tag="VFV87_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104702.1"
                     /translation="MGLRLAILTLLGLLPFALAKAGGAASPASDGAKDIAELIPQLGS
                     AEFARRQAASQELTEAGKAVFPELERAALEGGREVSGRAIDILKTHFNRGDDSTKQAA
                     QGALERLAKSGQAGAAQRASEALNPPQEPDAAFNGPFGAVPIFRGANIQVQMAARNAF
                     NRRVSIRDVNGQREIEVNENGKKTKITKNANGQIEAEITEQQNGKEVTRKVEAKDLDE
                     LKKKDADAAKIFEQYGQNARGNIQIQAGFAPALPALPAIPRIAGPPAAPNADAIKRMI
                     ESVEGRIEREKAQAQNNPNAQRIVESLERTRDRLKSMLPAEANPPAEKPAEPAPQPAE
                     KPTDN"
     gene            2330..3424
                     /locus_tag="VFV87_12870"
     CDS             2330..3424
                     /locus_tag="VFV87_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5104703.1"
                     /translation="MTVFAARREALRRILIDKSLAALLVTDERNVTYLTGFTGDSSYL
                     VVSRDHELLLSDRRYSQQLEEECPGLPLAIRGPGTKMTEFTAEVAGKLELSSLGVESE
                     AVTVGAFEKFKEALTTTPLALTDGLVESLREIKDAGEIEEIREAVSIAQRAFAVIRAS
                     LRVGRTEKELADELEHQIRLFGGTCGAFPSIIGVGPRAALPHGRPIRDSKIGDDDFVL
                     IDWGARGRLYHSDLTRVLVTGRLSPELQKVYGVVLAAQKAAIAAIRPGAIMKEVDATA
                     RRVIADAGYGERFGHSLGHGIGLAVHEQPRLAPDQDRQLAAGMVVTVEPGIYLPGWGG
                     VRIEDDVLVTPDGHELLTSVPKELEESLAH"
     gene            3550..4050
                     /gene="accB"
                     /locus_tag="VFV87_12875"
     CDS             3550..4050
                     /gene="accB"
                     /locus_tag="VFV87_12875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5104704.1"
                     /translation="MSEPEAPAGSVFDTDRISRLVELMKKHDLSEIDLRESRQRIRIC
                     RGPKEAPRPAYGPPPPPPPPGPSMPAPPGQPAAAGPAADGPNIAVIKCPMVGTFYSRP
                     NPKAEPFVKVGDRVDTSTVVCIIEAMKVFNEIPAEVRGKVVAVLANDGEGVEFDKPLF
                     KVDTSG"
     gene            4141..>5160
                     /locus_tag="VFV87_12880"
     CDS             4141..>5160
                     /locus_tag="VFV87_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5104705.1"
                     /translation="MYKRILIANRGEIALRIIRACQELGIETVCVYSTADAGSQYLQL
                     ADEAICIGPPKSAQSYLKIDQIISAAEVGNVEAIHPGYGFLAENAHFNEVCRSCNIDF
                     IGPTPQAMEALGDKNAARKMARQANVPVVPGSAGLIEDEAEAVRTAHEIGFPVLIKAT
                     AGGGGKGMRVAGNDLSLKTALQQAKTEAQAAFGNAGVYLERFVERPRHVEVQIIADHH
                     GNVCHLFERECSTQRRHQKLIEESPAARLPADKRLAMCEAAVRLVKEAGYTNAGTVEF
                     IVDQQENFYFIEVNARIQVEHPVTEMITGIDLVKSQIRVASGEPLPFRQEDIPQNGAA
                     IECRIN"
BASE COUNT         1020 a         1648 c         1610 g          882 t
ORIGIN      
        1 cagcaaggct gtcacaagtc ggaccttggc ctggtcaggc tgatggcgcc tgatcgcact
       61 aaaacagcgt ctcatcgggc tcgtggccga tgagttcgcg ggcgcgctgg aggtcttttt
      121 tcgcgtcttt ttcccggccc agggtcttca gtaccagggc gcggtgataa tggattacgg
      181 caaccccctg ttcaggtttg ttcagcccgg tcagatcgat atcggaggat agcacgcttc
      241 ggcctcgttc gggacgcgga ttgtcaattg ccgagaattc ctcctcgact cctttcacgg
      301 cggcgtccat atcgacgatg gctttttcgt tctcgccgtt caggtgccgc aggtatccgc
      361 gggtgtcgag aattgccgca ctttttggtt ccagctcgag ggcctcagca acgttcttca
      421 aggcttcatc cagttccagc ctgccgacgg cccgtgcgta cgcgagcccg ttgagagcca
      481 tcgcccgaga aggcgtgccg cgagtcaagc tgaggcggtc gatcgccttc cagtcctgga
      541 tggcctcggc atggcggtcg agttgctgca aaatctgcga acgcaggtaa aggacggtga
      601 aattgtcgcg ggccagttca gcggcctgat tcgcgtcttc cagggccttt tcagcctggc
      661 tgtcttccag ccgccaggcc gccctctgga gcaataatcc ggcgtgtttg ggcgaccagc
      721 gaatcgcctc cgccagccgg ttgtacgcct cgtccttttg tcccgatgag cgaagctcct
      781 gggcagacgc cagataccag tgtgcgattt cgcgcggcgc aagcaccgcc gcgcaggggg
      841 cgactagcaa agcaatcaac acgaccacga tcagccgtgg cccccaccgc ggccgcttgg
      901 gcgcctgatc gggaaacggg cccacagggc caggggcggg cggcggcacg ggcgtcggct
      961 ggccgggagg cggatcaaat ggcggatatg taggtcgatt ttctgagggt tggctcattc
     1021 ggcgccagac ggaggtttga cgaacaggcg gctggtgaat tctactagaa cctccaaatc
     1081 gggcctacca cttgcccgat cggacaaatt gacgcccctc ggctggtgtt tccctttaaa
     1141 cacttgggcc aaggatgagg ataaagattt tgcccgtcac acggcttgct gctccacaga
     1201 tggggcgggc taagatacga taatattcac ttcgccaggg tcattcgcgc tcgggtctcg
     1261 attcacgacc gtcgtcgctg gaggtcgttg ccatgggtct tcgactagca atactaaccc
     1321 tccttgggtt gctcccgttc gcacttgcca aagccggggg tgccgccagc ccggcgtccg
     1381 atggcgccaa ggacatcgcc gagctgattc cgcagcttgg ctctgccgaa tttgcccgcc
     1441 gccaggccgc ttcgcaggag ctgacggaag cgggcaaagc ggtttttccc gaactggaaa
     1501 gggccgcctt ggaagggggc cgcgaggtct ccggccgggc gatcgacatc ctcaagacgc
     1561 atttcaatcg cggtgacgac tccaccaagc aagcagccca gggcgcactt gagcggctgg
     1621 cgaagtcggg ccaggccggc gcggcgcagc gggccagcga ggccctcaat ccgccgcagg
     1681 aaccggacgc agcgtttaac ggcccgttcg gcgcagtgcc aattttccgc ggggcgaaca
     1741 ttcaggtgca gatggccgcc cgcaacgcgt tcaatcgccg cgtgtcgatc agggacgtca
     1801 acggtcagcg cgagatcgag gtgaatgaga acggcaagaa gacaaagatc acgaaaaacg
     1861 ccaacggcca aattgaagcc gagatcaccg agcagcagaa cggcaaggaa gtgacgcgca
     1921 aggtggaagc gaaggacctg gatgagctga aaaagaagga tgccgacgcg gcaaagattt
     1981 tcgagcagta cggccaaaac gctcgcggga acatccagat ccaggccggc tttgcaccgg
     2041 cgttaccggc attgcccgcg attccacgaa ttgccggccc accggcggcg ccaaatgcag
     2101 atgcgatcaa gcggatgatc gaatcggtcg aagggcggat cgagcgggaa aaggcccagg
     2161 cccagaacaa tcccaacgcc cagcgaattg tcgaatctct cgagcgcacg cgggaccgcc
     2221 tgaagtccat gttgccggcg gaagccaacc ctcccgccga gaagcccgcg gaacctgcgc
     2281 cgcagccagc agagaaaccc actgacaact gacccgtgac aagcatcgaa tgaccgtctt
     2341 tgccgcccgc cgcgaggccc tccgccgcat tctcattgac aagtcgcttg ccgcactcct
     2401 tgtcacggac gagcgcaacg ttacgtacct gacggggttt accggcgact cgagctacct
     2461 ggtcgtcagc cgtgatcacg agttgctgct ctccgatcgc cgctattcgc agcaacttga
     2521 ggaagaatgc cccggactgc cgctggcaat tcgcggcccc ggtacgaaga tgacggagtt
     2581 caccgcagag gtggccggca agttggaatt gtcgtccctg ggtgtcgagt cggaggcggt
     2641 gaccgtcggc gcgtttgaaa agttcaaaga agccctgacg accacaccgc tggcgctgac
     2701 cgatgggctt gtcgagtcgc tccgcgagat caaggacgcc ggcgagatcg aagagatccg
     2761 cgaggcggtg agcatcgccc agcgggcctt tgccgtcatt cgcgcgtcgc tgcgggtcgg
     2821 gcggaccgaa aaggaactgg ccgacgagct ggagcatcag attcgcctat tcggtggaac
     2881 gtgcggcgcg tttccgtcga tcatcggcgt cggcccccgg gctgcactcc cgcacggccg
     2941 gccgattcgc gactcgaaga tcggcgacga tgacttcgtg ctcatcgact ggggggcccg
     3001 cggcaggctc tatcacagcg acttgacgcg ggttttagtg accggtagat tgtcgcccga
     3061 actccagaaa gtgtatggag ttgtgctcgc cgcccagaaa gccgccatcg ccgcgattcg
     3121 gcccggcgcg atcatgaagg aggtcgatgc taccgctcgt cgggtgattg ccgacgccgg
     3181 ctatggcgag cggttcggcc attcgctggg gcacggcatc ggcctcgccg tccacgagca
     3241 gcccaggctc gcgcccgatc aggaccgcca gctcgccgca ggcatggtcg tcaccgtcga
     3301 gccgggcatt taccttcccg gctggggcgg cgtgcgaatc gaagacgacg tgctcgtcac
     3361 cccggacggc catgaactat tgacgagcgt gcccaaggaa ctcgaagagt cgcttgctca
     3421 ctgactcctg tcccctgact cctgtctcct tgccgcatgc ccgaccccga caaaccccgc
     3481 tcccgccggc aagctggcaa gaagccgcct ccgccttctc cttctcgaac accgccccgg
     3541 aagaacccga tgagtgaacc ggaagctccc gctggcagcg tctttgatac ggaccgcatc
     3601 agccggctgg tcgaactgat gaagaagcac gacctgtcgg agatcgacct ccgcgaatcg
     3661 cgccagcgga tccgcatctg ccgcggccca aaggaggcgc cgcggccggc ctacggtcca
     3721 ccgcctccac cgcctccgcc cggaccatca atgcccgcgc cgcccggcca gccagccgcg
     3781 gcgggtccgg cagccgacgg cccgaacatc gcggtcatca aatgcccgat ggtcggcacc
     3841 ttctattccc gccccaaccc caaagccgag ccgtttgtga aggttggcga ccgcgtcgat
     3901 accagcaccg tcgtctgcat catcgaggcg atgaaggtct tcaacgagat tcccgccgaa
     3961 gtccgcggca aagtcgtcgc cgtcctggcc aacgacggtg aaggggtcga gttcgacaag
     4021 ccgctcttca aggtcgatac cagcggctaa tggatgcgga attcagaact cagaattcag
     4081 aatgcagaat gatgaatctg agttcccttt ctgaattctg cattctgcct tctgaattcg
     4141 atgtataagc gcattctcat cgcgaaccgc ggcgaaatcg ccctgcggat catccgcgcc
     4201 tgccaggagc tggggatcga gacggtctgc gtctatagca cggccgacgc gggcagccag
     4261 tacctgcagc tcgccgacga ggcgatctgc atcggcccgc ccaaaagcgc ccaaagctat
     4321 ctgaagatcg accagatcat cagcgcggcc gaggtcggca atgtcgaggc cattcatccg
     4381 ggctacggct ttctggcgga gaacgcgcac ttcaacgaag tctgccggag ctgcaacatc
     4441 gacttcatcg gcccgacgcc gcaggcgatg gaggccctcg gcgacaaaaa cgccgcgcgc
     4501 aagatggccc gccaggcgaa cgtgcccgtg gtgcccggca gcgccggcct gatcgaggac
     4561 gaagccgaag cggtccgcac ggcccacgag atcggctttc cggttttgat caaggcgaca
     4621 gcggggggcg gcggcaaggg gatgcgcgtc gccggcaacg acctctcgct gaagaccgcc
     4681 ctgcagcagg ccaagacaga agcccaggcg gcgttcggca acgcgggcgt ttatctggag
     4741 cggttcgtcg agcggccgcg gcacgtcgag gtgcagatca tcgccgacca tcacggcaac
     4801 gtctgccatc tctttgagcg cgagtgcagc acgcagcgcc gccaccagaa gctgatcgaa
     4861 gaaagccccg cggcgcgcct gccggccgac aagcgcctgg cgatgtgcga agcggccgtc
     4921 cgcctggtca aggaggcggg ctacacgaac gccggcacgg tcgagttcat cgtcgaccag
     4981 caagaaaact tttacttcat cgaagtaaac gcccgcatcc aggtggaaca tcccgtcacc
     5041 gagatgatta cgggcatcga cctggtaaaa tcccagattc gcgtggcctc tggcgagccg
     5101 ctgccgtttc gccaagaaga cattccccaa aatggcgcgg cgatcgaatg ccggatcaac
//
LOCUS       DASUGB010000673         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2021186, whole genome shotgun sequence.
ACCESSION   DASUGB010000673 DASUGB010000000
VERSION     DASUGB010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021186"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFV87_12885"
     CDS             complement(<1..1304)
                     /locus_tag="VFV87_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104706.1"
                     /translation="MSTTTTSSAEHQTRKASPARSSRRFPGPKTRLAIAMGLVLTGLF
                     RVLWRVESLPFPLSDRAFGNMMSFLFGALAVLLAWQWFAFHSDYSKAARRTVMFGGIG
                     ALALFFAIFKLDGFRGDMVVERFRLRWLPAADRQLDEIISLDDGKPIDLTTTTPADFP
                     EFLGPGRRCWIEDPGLATDWTAQPPKLLWKHEIGAGWSAFAAVNGYAVTMEQRGDQEW
                     VSCYEIATGKPIWGRAIEARHAHPLGGVGPRSTPTIAGGHVYALGGTGRLQCLDGSNG
                     KLIWEQDLLQLYGVTQAESEAEVLWGRAASPLVVDGLVIVPAGGKTDPANKRKPTSLV
                     AFRAEDGTKVWEAGADQISYSSPVLATLAGQRQVVIVNEMTVTGHDVETGRQLWGHKW
                     PGSSSSSASVSQAVPLAGDMLLLTKGYGGGAELLKLVAGGSGS"
     gene            complement(1416..2315)
                     /gene="lpxI"
                     /locus_tag="VFV87_12890"
     CDS             complement(1416..2315)
                     /gene="lpxI"
                     /locus_tag="VFV87_12890"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913214.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX5104707.1"
                     /translation="MTSNPQSANCNPQSLRIGLVAGWGRYPIVVAQTLKAQGKQVYCV
                     GLENHADPSLATICDDFVSRGVARIGQHIRYFRRQGVTHATMAGKVFKHKILFGRFGW
                     LGLLPDWRTIRVAFPLYILGRRDRRDDTLLSMAVQEYAKDGIIMAPATDLAPQLLARE
                     GQLTRRGPTKGQWQDIEFGWNLAKELGRLDVGQSVTVKGRAVIAVEAVEGTDACIRRS
                     GEFCPQGGFTVVKVAKPQQDMRFDVPTIGVGTIESMLAARASVLAIEASKTIIVDEPD
                     VVSLADKHGLAIVALCSDHIPAR"
BASE COUNT          430 a          794 c          745 g          451 t
ORIGIN      
        1 gaaccgctgc cgccggcaac gagcttgagc agttccgccc cgccgccata gcccttcgtc
       61 aggagcagca tgtcgcccgc cagcggcacc gcttgcgaca cgctggcgct ggagctgctg
      121 ctgccgggcc acttgtgccc ccaaagctgt cggcccgttt ccacgtcgtg tccggtcacc
      181 gtcatctcgt tgacgatcac cacctgccgc tgcccggcca gcgtcgccag caccggtgac
      241 gagtagctga tctggtcggc gcctgcctcc cagactttgg ttccgtcctc ggcccggaag
      301 gcgacgagcg atgtcggctt ccgtttgttc gccggatcgg ttttgccgcc cgccggcacg
      361 atcaccagtc catcgaccac gagcggcgat gccgcccggc cccacaggac ttcggcttcg
      421 ctctcggctt gcgttacgcc gtatagctgg agcaggtcct gctcccaaat cagcttgcca
      481 ttgctcccat cgaggcactg caacctgccg gtcccgccca gggcatagac gtggccaccg
      541 gcgatggtcg gcgtcgaacg cggtccaacg ccccccagcg ggtgcgcgtg acgggcttca
      601 atcgcgcggc cccaaatggg cttgccggtc gcaatttcgt agcacgaaac ccattcctgg
      661 tcgccgcgct gctccatcgt caccgcgtag ccgttgaccg cggcgaaagc cgaccagccc
      721 gcgccgatct catgcttcca aagcagcttc ggcggctgcg cggtccagtc ggtcgccagg
      781 cccgggtctt cgatccagca gcggcggccg gggccgagga attccgggaa gtcggcgggg
      841 gtcgtggtcg tcaggtcgat cggtttgccg tcgtctaagg agatgatttc atcgagttga
      901 cgatccgcag caggcaacca gcgcagccgg aacctttcga ccaccatgtc gcctcgaaag
      961 ccgtccaact tgaagattgc gaaaaataaa gccaaagcgc cgatgccgcc gaacattaca
     1021 gtccgccgcg cggcctttga ataatcgctg tgaaaggcga accattgcca cgccaagagc
     1081 actgccagcg ccccaaacag gaacgacatc atgttgccga acgcccgatc actgagcgga
     1141 aaggggagac tttccacgcg ccacagcaca cggaatagcc cggtgaggac cagccccatg
     1201 gcaatcgcca gccgcgtctt cggacctgga aatctccgcg acgaccgcgc gggtgacgcc
     1261 ttgcgggttt gatgctctgc agacgaagtg gttgtagtac tcatgaatgc tgcgtatcgg
     1321 cgctggttcg cgtcattccc catcagattg tatgacgcga cgcttgtttg aaaccgtctc
     1381 caatcgtgaa aaaccaagaa ctcgtcaatc gtctattatc ttgccgggat atgatcggag
     1441 caaagggcca cgatcgccag gccgtgcttg tcagccaggg acacgacatc gggctcatcc
     1501 acgataatcg tcttgctggc ttcgatggcc aggacgctgg cgcgggcggc gagcatcgat
     1561 tcgatcgtgc cgacgccgat cgtaggcacg tcgaaccgca tgtcctgctg cggcttggcg
     1621 accttgacga cggtgaagcc tccctgcggg cagaactcgc cgctgcggcg gatacaggcg
     1681 tcggtccctt cgaccgcctc gacggcaatc accgcgcggc ccttgacggt gacgctttgc
     1741 cccacgtcga gccggccaag ttctttggcc aggttccagc cgaattcgat gtcctgccac
     1801 tggcccttcg tggggccgcg gcgggtgagt tgtccttcgc gggccagaag ctgcggagcg
     1861 agatcggttg ccggcgccat gatgattccg tccttggcgt attcctgaac ggccatggat
     1921 aagagcgtgt cgtcgcgtcg gtcgcgacgg cccaggatat agagcggaaa agcgacgcgg
     1981 atcgtgcgcc aatccggcag cagtcccagc cagccgaatc gcccgaacag gattttgtgc
     2041 ttgaagactt tgccggccat cgtggcgtgc gtgaccccct ggcggcggaa ataacggata
     2101 tgttgtccga tccgggccac gccgcgggat acgaagtcgt cgcagatcgt ggcgaggctc
     2161 gggtcggcgt ggttctccag gccgacgcaa tacacttgtt ttccctgggc cttcaatgtc
     2221 tgagccacga cgatcggata ccgcccccac cccgcgacga ggccaatgcg gagggattgc
     2281 ggattgcaat ttgcggattg cggattactc gtcattccgc ctaggctgcg tcgccgtgaa
     2341 tgttgctgtc cgaactggcg gcggcggaga tgccactctc gcccgcgagc agagccaact
     2401 gccgctgcag ctcgtggatt
//
LOCUS       DASUGB010000674         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_215622, whole genome shotgun sequence.
ACCESSION   DASUGB010000674 DASUGB010000000
VERSION     DASUGB010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215622"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFV87_12895"
     CDS             complement(<1..688)
                     /locus_tag="VFV87_12895"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium uptake protein TrkA"
                     /protein_id="HEX5104708.1"
                     /translation="MLDWLLELPQTSPVGHAIGLLALVCAVGMAVGSIKWKGIGLGSS
                     GVLFVGILVGQWSDPIDAHTLNFVKEFGLVLFVFTLGLQLGPGFFASLRADGLKLNVL
                     AGLLVVFAGILTPLVGWLAGMDPAAVAGLFAGASTNTPALGAAQQSLASMPDISSQQQ
                     ALTALACAVSYPAAIAGSLGVILAVKMLFRIDPVQEAADYAAQRRQAIPPLHRRTLVV
                     DQPEMWAILCE"
     gene            complement(726..2120)
                     /locus_tag="VFV87_12900"
     CDS             complement(726..2120)
                     /locus_tag="VFV87_12900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5104709.1"
                     /translation="MKIKLVGASGGEVTGSAYVVRTKRATIMVDAGMFQGGRQSEAKN
                     RLPSGVRPDECQAVLLTHAHLDHSGRLPLLIKHGFSGPVYATAATIDLAELILKDCAR
                     IQSQDAERKTRKAARNDLPPVEPLYEPEDVEPFRSLARKVQFQKPVEVAEGISARWVE
                     AGHMLGSGCIELTVDEDGRKKVVVFSGDLGPTTLPLIREYQIFDRADAVFLESTYGDR
                     LHRPYAQTVAEFERIVKQVAKGTGKILVPTFAIGRSQQMLYHLAIMFLRKDIRPFPVF
                     LDSPMALEATKIYRKYPDLFDDELIAWQKKGLLPLNRSIFKPSVTANDSKKINDVPGP
                     CVVLAGAGMCNAGRILHHLKFNLDNPTTHVLIVGFQGHGSLGRRLVEGQKVVTIFGER
                     IPVRASVHTLNGFSGHADQGELLKWFSTLAPAKPKVILTHGEDRGRGALARLIQQRFK
                     LTAALPKQGDVIEI"
     gene            complement(2169..2972)
                     /locus_tag="VFV87_12905"
     CDS             complement(2169..2972)
                     /locus_tag="VFV87_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5104710.1"
                     /translation="MTKSNESIALDAWEDAWQKHWKKTGFTSYEMHLPDTNAANRERN
                     FLTQNRSAERETARLKRITAEFERGFKRLGKLGPAVTVFGSARFRPGHPHYELAREVG
                     RELALAGFTTITGGGPGAMEAANRGAHETGGPSYGLNIILPHEQAANPYVDESVEFQY
                     FFVRKVMLVKYSCAYIVVPGGLGTLDELYEAATLIQCGKIGPFPLILLGREFWQGMKD
                     FLLFMVEQGVFDAKEIGFARIVDSPAEAVEMVVRAIPESRREHLKPLKK"
     gene            complement(2989..>3669)
                     /locus_tag="VFV87_12910"
     CDS             complement(2989..>3669)
                     /locus_tag="VFV87_12910"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5104711.1"
                     /translation="VPKTIDNDLAATMMTFGFDSAVACATDAIDRLHSTAESHRRVMV
                     LEVMGRYAGWIAIYAGLAGGADVILIPEIPFQYESVAKKIAEREQAGKHFSIVVVAEG
                     ARASGDDFVTSAGQPQQREARLGGIGQRVSDEIQRRTGKESRCVVLGHLQRGGSPTNM
                     DRALCTAFGAKAVELVAERKFGQMVSLAGSQITGVPLAEATGKLRTVPLDGGFVTAAR
                     SMGICLGD"
BASE COUNT          679 a         1199 c         1126 g          665 t
ORIGIN      
        1 gctcgcaaag gatggcccac atttcgggct gatcgacgac gagcgtccgc cggtgcagcg
       61 gcgggatcgc ctgccgccgc tgggctgcat agtcggcggc ctcttgcacc gggtcgatac
      121 gaaacagcat cttcaccgcc agaatgacgc ccagcgaacc agcaatcgcc gccggatatg
      181 agacggcaca ggccaatgcc gtcaatgcct gctgctgact gctaatgtca ggcatcgaag
      241 ccagcgactg ctgcgcggcg ccaagcgcgg gcgtgttggt cgaagccccg gcaaacaacc
      301 ccgcgacagc cgcgggatcc atgccggcca gccatcccac cagcggcgtc aggattcccg
      361 caaatacaac caacaaacca gccagcacat tgagtttcag tccgtcggcc cgtagcgaag
      421 cgaaaaagcc cggtccgagc tgcaggccga gcgtaaaaac aaacagcacc agtccgaatt
      481 ccttgacgaa attgagcgtg tgcgcgtcga tcggatcgct ccattgcccg accagaatgc
      541 cgacgaagag cacgcccgag ctgcccagcc cgatcccctt ccacttgatg ctccccaccg
      601 ccatgcccac tgcgcaaacc agcgcgagca ggccaatcgc atggcccacc ggactggttt
      661 gcggcaattc caggagccag tccagcatgg gggtcggtcc atcgagggta ttcgcccggc
      721 cagccctaaa tctcgatcac atccccctgc ttcggcaagg cggcagtcag cttgaaccgc
      781 tgctgaatca gcctggcaag tgccccgcgg ccgcggtcct caccgtgggt gaggatgacc
      841 ttgggtttgg cgggcgccaa ggtcgagaac cacttgagca gctcgccctg gtccgcatgc
      901 ccgctgaagc cgttgagcgt gtggacgctg gcccgtaccg gaattctctc gccgaagatc
      961 gtgacgacct tctggccctc caccagccgc cggcccagcg aaccatgccc ctgaaagccg
     1021 acgatcagga cgtgcgtggt cggattgtcg aggttgaact tgaggtggtg caggatacgt
     1081 ccggcgttgc acatccccgc gccggcgagc accacgcagg gccccggcac gtcgttgatc
     1141 ttcttcgagt cgttcgccgt cacgctcggc ttgaagatcg agcggttcag cggcaagagg
     1201 cccttctttt gccaggcaat cagctcgtca tcaaagaggt cgggatactt gcggtagatc
     1261 ttggtggcct ccagggccat cgggctgtcg aggaagaccg ggaacggccg gatgtccttg
     1321 cggaggaaca taatcgccag gtgatacagc atttgctgcg atcggccgat ggcaaacgtc
     1381 ggcacgagga ttttgccggt gcccttcgca acctgcttca cgatccgctc gaactcggcc
     1441 accgtctgag catacggccg gtgcaggcgg tcgccgtagg tcgattcgag gaagaccgca
     1501 tcggcccggt cgaagatctg atattcgcgg atgagcggca gcgtcgtcgg ccccagatcg
     1561 cccgaaaaga caaccacttt cttgcggcca tcctcgtcga ccgtcagctc gatgcagccg
     1621 gagccgagca tgtgccccgc ttcgacccag cgggccgaaa tcccctcggc cacctccacc
     1681 ggcttttgga attgaacctt gcgcgcgagc gagcgaaacg gctccacatc ttcgggctca
     1741 tacagcggct cgacgggcgg cagatcgttc ctggcagcct tgcgcgtctt gcgttcagca
     1801 tcctgcgact ggatgcgggc gcagtccttc aggatcagct cggccaaatc gatcgtcgcc
     1861 gccgtggcat acaccgggcc ggaaaacccg tgcttgatca agagtggcag ccggccgctg
     1921 tgatcgaggt gggcatgtgt cagcaggacg gcctggcact cgtctgggcg aactccggac
     1981 ggcaggcgat tcttggcctc gctctgccgg ccgccttgaa acatgccggc gtcgaccatg
     2041 attgtcgccc gcttggtgcg aacgacataa gccgagccgg taacttcgcc gcccgaggcg
     2101 ccgacgagct tgatcttcat ggagtcacat cgcgagtcga ggagcaggag agtaaatgcc
     2161 agcggttact acttcttcag aggttttagg tgctcgcgtc tcgattccgg aatggcgcgc
     2221 acgaccattt cgacggcttc ggcgggggaa tcgacaatgc gggcaaagcc gatctccttg
     2281 gcgtcgaaga cgccctgctc gaccatgaac agcaagaagt ctttcatccc ctgccagaac
     2341 tcgcggccca ggaggatcag cggaaagggg ccaatcttgc cacactgaat gagcgttgcc
     2401 gcttcgtaca gctcgtcgag cgtccccagg ccgcctggca cgacgatata agcacacgag
     2461 tacttcacca gcatcacctt gcgaacaaag aaatactgga attccacgct ttcatcgacg
     2521 tacggattgg ccgcctgctc gtggggcagg atgatgttca ggccatacga cggcccgccg
     2581 gtctcgtgcg cgccgcggtt ggcggcttcc attgcccccg gcccgccgcc ggtaatcgtg
     2641 gtgaagccgg ccagggccag ttcgcgtcca acttcgcggg ccagctcata gtgcggatgc
     2701 cccggcctga agcgggcgga gccgaagacc gtcacagccg gcccaagttt gccgagccgc
     2761 ttgaacccgc gctcgaactc ggccgtgatc cgcttgaggc gagctgtttc gcgttcggcc
     2821 gagcggttct gcgtcaggaa attgcgctcg cgattggccg cattggtatc gggcaggtgc
     2881 atctcgtagg aagtgaagcc cgttttcttc caatgctttt gccacgcgtc ttcccacgcg
     2941 tccagtgcga tcgattcgtt cgacttggtc atgattttca ctctgaattc agtcgccgag
     3001 acagatcccc atcgaccgtg cggcggtgac gaagccgcca tccagcggga cggttcgcag
     3061 cttgcccgtc gcttcggcca gcggaacgcc ggtgatctgg ctgccggcga gcgaaaccat
     3121 ctggccgaac tttcgctcgg cgaccagctc gaccgctttg gccccgaacg cggtgcagag
     3181 ggcccggtcc atgttcgtgg ggctgccgcc gcgctgcaaa tggcccagca ccacgcagcg
     3241 agactctttt cccgtgcgcc tttggatttc gtcggagacg cgctgcccga tcccgcccag
     3301 gcgggcctcg cgctgctgcg gttggccggc ggacgtgaca aagtcgtcgc cgctggcgcg
     3361 cgcgccttcg gcgacgacca cgatcgaaaa atgcttgccc gcttgttcgc gctcggcgat
     3421 cttcttcgcg acactctcgt attggaacgg gatttcggga atcaggatca catcggcccc
     3481 gccggccagg cctgcgtaga tcgcaatcca gccggcatag cggcccatca cctccagcac
     3541 catgacgcgg cggtggcttt cagccgtgct gtgcagacgg tcgatagcgt cggtggcaca
     3601 ggcgacggcc gaatcgaagc caaacgtcat catcgtggcg gctaggtcgt tgtcgatcgt
     3661 cttcggcac
//
LOCUS       DASUGB010000675         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1464183, whole genome shotgun sequence.
ACCESSION   DASUGB010000675 DASUGB010000000
VERSION     DASUGB010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464183"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..173
                     /locus_tag="VFV87_12915"
     CDS             6..173
                     /locus_tag="VFV87_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104712.1"
                     /translation="MLTLARVMMQAALAREESRGVHLRTDFPKLDDAHWQKHLWFERS
                     EAADSEQWGAA"
     gene            176..>2636
                     /locus_tag="VFV87_12920"
     CDS             176..>2636
                     /locus_tag="VFV87_12920"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5104713.1"
                     /translation="MRVVCLLLPLISATTLAAESVQPAAAIFERRCVSCHGAAEPKGG
                     LSLASLKAILAGGDSGAVVVPGKPNESLLLEYVTGEKPEMPKSGPPLTAEEVAAIRDW
                     IAAGSKLPDDLTLQDRSGPDANWWSLQPLTRPPVPTIQNPQSTIRNPIDAFIVAQLAE
                     MALPQSPSADRRTLIRRLYFDLIGLPPTPEELDRLLSPSQRQEGEREREREWEKTYER
                     LVDQLLASPHYGERWARHWLDVVHYGETHGYDKDQPRPNAWPYRDYVIRAFNGDKPYG
                     RFVEEQIAGDVLYPGTADGIEALGFIAAGPWDLIGHAEVPETKIDGKVARHLDRDDMV
                     ATTIGTFNSLTVGCAQCHNHKFDPIPQADYYRLQAVFAAVDRADKLYDRDPAVAKRRR
                     ELTRQLAERSSEKQAIDSAVAKRGGTPLAELDQQIEALTKVSGGKERPEFGYHSAIET
                     KQDAVKWVQVDLSSSQPIARIEYVGCHDDFNHIGAGFGFPLRYKIEVSDDADFKTGVN
                     VVVERTQADIPNPGVTPQTVSLQDTKGRFVRVTATRLAPRLPTDFIFALAELSVFMQD
                     GENVASGAEVASLDSIEAPVRWSRKNLVDGYYFGQATGAAEKIAQLTRQRDALLEMVL
                     DEDTKARLGEIQTAIAKVEQELNALPPQQRVYAGTVHHGSGNFLGTGASGGQPRAIFI
                     LNRGNVTQPREEVEAGALSCLPVEFPLAGSKLAPGEPATDNTPGPGLVPAKSEGLRRA
                     ALAKWLADPNNPLTWRSIVNRIWQYHFGRGIVDTPNDFGHMGALPTHPELLDWLAVEF
                     RDGDQSLKRLHKLIVMSHTYRQ"
BASE COUNT          517 a          841 c          855 g          423 t
ORIGIN      
        1 agaacatgct cacgctggcc cgcgtgatga tgcaggcggc gctggcccgg gaggaatcgc
       61 gcggcgtcca cctgcgcacg gacttcccca aactcgacga cgcgcactgg cagaagcacc
      121 tgtggttcga gcggagcgaa gccgccgaca gcgagcagtg gggggcagcc tagcgatgcg
      181 agtcgtatgc ctgctgctgc cgctgatttc ggccacgacg ctggcggcgg agtccgtgca
      241 accagccgcg gcgatcttcg agcgccgctg cgtgagttgc cacggcgcag ccgaaccgaa
      301 aggcgggctg tcgctggcct cactcaaggc cattctcgct ggcggagata gcggcgcggt
      361 cgtcgtgccc ggcaagccga atgagagtct gctgctcgaa tacgtcacgg gcgagaagcc
      421 ggagatgccc aagtccggcc cgccgctgac cgcggaggaa gtcgcagcca ttcgcgactg
      481 gatcgccgcc ggctcgaagc tgccggacga tctcacgctg caagaccgca gcggccccga
      541 cgcgaactgg tggtccctgc agccgctcac tcgacctccc gtgccaacaa tccaaaatcc
      601 acaatccaca atccgcaatc cgatcgacgc gttcatcgtc gcccagctcg ccgaaatggc
      661 cctgccgcag tctccctccg ctgaccgacg cacgctcatc cgccggctgt atttcgacct
      721 gatcggcctg ccgccgacgc cggaggagct tgaccggctt ctctccccct cgcaacgcca
      781 agagggggag agggagagag agagagagtg ggagaagacg tatgagcggc tcgttgacca
      841 gcttcttgcg tcaccccact atggcgagcg ctgggcgcgg cactggctcg atgtcgtcca
      901 ctacggcgag acgcacggct acgataagga tcagccccgg ccgaatgcct ggccctatcg
      961 cgactacgtc atccgggcct tcaacggcga caagccctac ggccgcttcg tggaagagca
     1021 gattgccggc gacgtgcttt atccggggac ggccgacggc atcgaggcgc tgggtttcat
     1081 cgcggcggga ccgtgggact tgatcggcca tgcggaggtg cccgagacga agatcgacgg
     1141 caaggtggcc cggcacctcg accgcgacga catggtcgcg acgacgatcg gcacgttcaa
     1201 cagcctgacc gtcggctgtg cccagtgtca taaccacaaa ttcgatccga tcccgcaggc
     1261 cgactattac cgcctgcagg ccgtgtttgc cgccgtcgat cgggccgaca agctgtatga
     1321 ccgcgatccg gcggtggcca agcgccgccg tgagttgacg cggcagcttg cggaacgatc
     1381 cagcgagaaa caggctatcg actccgcagt cgcgaagcgc ggcggcacgc cgctggcgga
     1441 gttagaccag caaattgaag cgttgaccaa ggtgtccggc ggcaaagaac ggccggagtt
     1501 cggctaccac agtgcgatcg aaacgaagca ggacgcagtg aagtgggtcc aggtcgatct
     1561 gagctcaagc cagccgatcg cgcgaatcga atacgtcggc tgccacgacg atttcaacca
     1621 catcggggcc ggctttggct ttccgctgcg gtacaagatc gaggtttcgg acgacgctga
     1681 ttttaagacg ggcgtaaacg tcgtcgtcga acgcacgcag gccgacattc ccaatccggg
     1741 cgttactccg caaaccgttt cgctgcagga cacgaaaggg cggttcgtcc gcgtgacggc
     1801 gacgcggctt gccccgcggc tgccgacgga cttcatcttt gcgctggcgg agctgtcggt
     1861 cttcatgcag gacggcgaaa atgtcgccag cggcgcggaa gtcgcctcgc tcgactcgat
     1921 cgaagcgccc gtgcgctgga gtcgcaaaaa cctggttgat ggctattatt tcggccaggc
     1981 aaccggcgct gcagagaaga tcgcccaact cacgcgccag cgcgatgcgt tgctggagat
     2041 ggtgctggat gaagatacga aagcccggct gggcgaaatt cagacggcga tcgccaaagt
     2101 cgagcaggaa ttgaatgccc tcccgccgca gcagcgggtc tatgccggca cggtccatca
     2161 cggcagcggc aacttcctcg gcaccggagc atccggcggc cagccgcggg caattttcat
     2221 cctgaaccgg ggcaatgtca cgcagccgcg tgaagaagtt gaagcggggg cgctgtcgtg
     2281 tttgcccgta gagttcccct tagccgggag caaacttgct cccggagaac cggcaaccga
     2341 taacacgccg ggaccaggtt tggtcccggc taagagcgag ggtttgcggc gcgccgcgtt
     2401 ggcgaagtgg ttagccgatc caaacaaccc gctgacgtgg cggagcatcg tcaaccggat
     2461 ctggcagtat cacttcggcc gcggcatcgt cgatacgccc aacgactttg gccacatggg
     2521 cgcgctgccg acgcaccccg aactgctcga ttggctggcg gtcgaattcc gcgatggcga
     2581 ccaatcgctc aagcggctgc acaagctgat cgtaatgagc catacgtatc ggcagg
//
LOCUS       DASUGB010000676         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1464654, whole genome shotgun sequence.
ACCESSION   DASUGB010000676 DASUGB010000000
VERSION     DASUGB010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464654"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="VFV87_12925"
     CDS             complement(<1..1847)
                     /locus_tag="VFV87_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5104714.1"
                     /translation="MRIAAVNVLMLIGVASCALAQDEHSVPAAIIERAAAKGRTAAAE
                     STTPGRMFALAAPRTVSLADLPEGTRLSLLNGAAAVHREVPADFASQGEWSTLPDGRR
                     AWRVAIHSPGAGSIRLHFTGFDVANGQVWLGSGTDSDAISGKPFTGQGPLGDGEFWSN
                     SVFADTVWVEFVPAADQTTDAIPFSVDQISHYFDVLRAPSGRAAQTSKSAANKAAPNG
                     VAACQLEAKCYTEYAGVASGIAQLQYESNGFGYVCSGALINSTSQRGIPYLLTADHCI
                     SNDAEARTLQSNFFYEANICGGATPETPILATHVLGGKYLASGGLDVGDYSLLLISDA
                     PDKTMFLGWTTADIALDSKVVGIHHPEGSYKRISFGHRVADEELVIGSGESAEYGPAN
                     RYWKVEFTEGLVQGGSSGSPLLNSANQVVGTLTAGPAFSNPEAEDVLICRLDPFEAFY
                     GRFANGFPSFRTYLEDAVPASMTSPANGAVLPGATVDFAWSKGTSSDDFKLYIGTTAG
                     GTQLAVENAGNSSFHRVNYLPTDGSRIFVRFWYRMLGTWRFTDYTYTAARDNGKKITI
                     SVTNRLLYPVQVKANGSNLMLVGGSAVATREIAVPSELVVTYDMIRPMV"
     gene            complement(1978..2475)
                     /locus_tag="VFV87_12930"
     CDS             complement(1978..2475)
                     /locus_tag="VFV87_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104715.1"
                     /translation="MVLTKQELIEKLQHEIQILVHLCDKVTPEMIDYRPTPKQRSLIE
                     LLRYLTVMGPVLVPSIKGGTFLAEQWGAAEAKAAEMDFAAVVKSLESQASFYAESISQ
                     FTDDELRGEIELFGFKSKRGPSLVSFVVCGHAAYRTQLFCYLKACGREELNTMNLWAG
                     IDATM"
     gene            complement(2505..>2939)
                     /locus_tag="VFV87_12935"
     CDS             complement(2505..>2939)
                     /locus_tag="VFV87_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104716.1"
                     /translation="LATHIVRLGKRVELSNIVVSSGLQAESLDEAWDHQVRWARTVRV
                     SRLDGYVGLPVTFTSLWAVAALAAGLWMPAAIALAARILMAAVTSTLVLRSTLVLRWL
                     PLLPLRDLMGVAVWIAGLTSNTVYWRGRKLRLDRDGRIAAIG"
BASE COUNT          608 a          896 c          866 g          569 t
ORIGIN      
        1 tccaccattg ggcgaatcat gtcgtaggtg actaccaact cgcttggaac cgcgatctcg
       61 cgagtggcca ccgcactgcc gccgaccagc attagattgc tgccgttggc ctttacctga
      121 accggataga gaagccgatt ggttaccgag atggtaatct tcttgccatt atcacgagcg
      181 gctgtgtagg tgtagtcggt aaagcgccat gtgccgagca tgcggtacca gaatcgtacg
      241 aagatgcggc tgccgtcggt gggcaggtag ttcacgcgat ggaagctgga attgcctgca
      301 ttctcaaccg ccagttgtgt gccgcccgcc gtcgtgccga tgtagagctt gaaatcgtcc
      361 gaagaagtgc ccttcgacca agcgaaatcc acggtcgcgc cggggagcac tgcgccattg
      421 gcggggctgg tcatgctcgc gggcaccgca tcttccagat aggtgcgaaa gctcgggaag
      481 ccgttcgcaa accggccgta gaaggcttcg aatggatcaa gcctgcagat cagaacgtcc
      541 tcggcttcgg ggttgctgaa tgccggcccg gccgtcaatg ttccaacgac ctggttggcg
      601 gagttcaata gcggcgaacc ggacgagccg ccctgcacga ggccttcggt gaattccact
      661 ttccaatacc ggttggccgg cccgtattcg gcactttcgc ccgaaccgat cacgagttcc
      721 tcgtcggcca cgcggtgccc gaagctgatg cgcttgtagc ttccctccgg atggtggata
      781 ccaaccacct tcgaatccaa agcgatgtcg gcggtcgtcc aacccaggaa catcgtctta
      841 tcgggagcgt ccgagatcag cagcagcgag tagtcgccta cgtcaagccc tccgctggcg
      901 agatacttcc cgcccagcac atgcgtggcg aggatcggcg tctccggcgt cgcaccgccg
      961 cagatgttgg cctcatagaa gaaattcgat tgaagcgtgc gcgcctcggc gtcattgctg
     1021 atgcagtgat cggcggtcag cagatacgga atgccgcgct ggctggtgga attgatcaat
     1081 gcccccgagc acacataacc gaaaccgttg ctttcgtact gcaattgggc gatgccactc
     1141 gcaacgccgg cgtactccgt gtagcacttg gcctcaagct ggcaagccgc tactccgttc
     1201 ggtgcagcct tgttggcggc agacttggat gtttgggcag cgcggcccga gggtgcacgc
     1261 aatacgtcga aatagtgcga aatctgatcg acggaaaaag ggatcgcgtc cgtagtctga
     1321 tcggcggcgg gaacaaactc cacccaaacc gtgtccgcaa acaccgagtt cgaccagaat
     1381 tcaccgtcac ccaatggtcc ctgcccggtg aagggcttgc cggagattgc atcggaatcc
     1441 gtgcccgatc ccagccatac ctgaccgttg gcgacatcga atcccgtgaa atgcaggcgg
     1501 atcgagccgg cgcccggaga atggatggca acacgccagg cgcgccgtcc atcgggaagc
     1561 gtcgaccatt cgccttgcga cgcaaaatcc gccggcacct cgcggtgcac cgcggctgca
     1621 ccgttcagaa gcgagagccg tgtgccttcc ggcaggtcgg ctagcgatac cgtacgtggc
     1681 gcggccagtg cgaacattct gccgggagtc gtcgattctg cagcagccgt ccggcccttt
     1741 gccgcagccc gctcgatgat cgctgccgga actgagtgct catcctgagc cagcgcgcac
     1801 gacgccacgc caatcaacat cagcacgttt acagcggcaa tccgcatgta gtcctccctt
     1861 aggcagagaa ggattcgcac cttctccttt cccaagttcg gccatttgca gaaaattcgc
     1921 gctaagccgg aagcctgagt tgactgtaga aacggcctga ggggccttcg ctcctattta
     1981 catcgtggcg tcgatacctg cccacaaatt catcgtgttc agctcttctc ggccgcaggc
     2041 cttcagatag caaaacagtt gagtccggta ggcggcatgg ccgcagacga caaagctgac
     2101 cagcgagggt ccgcgcttgg acttgaagcc aaacagttcg atctcaccgc gaagttcgtc
     2161 gtcggtgaac tggctaattg attcggcgta aaaagatgcc tgcgattcca gcgatttcac
     2221 cacggcggcg aagtccatct cggcggcttt ggcttcagcg gcgccccact gctcggcgag
     2281 aaacgtcccg ccctttatgg aaggtacgag caccgggccc attacggtca ggtagcgcag
     2341 aagttcgatc aggctgcgct gtttcggcgt ggggcggtaa tcgatcattt ccggggtcac
     2401 cttgtcgcaa aggtgcacga gaatctggat ttcgtgttgg agcttttcga tgagttcttg
     2461 cttggtcagt accataggtc ttcccaagtg tagcgcctgc gttgctatcc aattgccgca
     2521 atacggccat cgcgatccag ccgcagtttc ctgccgcgcc agtaaactgt attgctcgtc
     2581 aatcccgcga tccagaccgc cacgcccatg aggtcacgta ggggcagtag aggcagccag
     2641 cggagtacga gagtcgatct caggacgagt gttgaggtga cagcggccat caagatgcgc
     2701 gcggcgaggg cgatggcggc cggcatccac aagccagccg caagcgccgc aaccgcccag
     2761 agagacgtga aggtaacagg cagcccgacg tatccatcaa gccgagatac ccgcacagtt
     2821 cgtgcccagc gcacctggtg gtcccaagcc tcatcgagcg attccgcctg cagccccgac
     2881 gaaacgacaa tattcgacaa ctccacacgt ttccccaggc gcacaatgtg cgtggccag
//
LOCUS       DASUGB010000677         4684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_75598,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000677 DASUGB010000000
VERSION     DASUGB010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75598"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="hisA"
                     /locus_tag="VFV87_12940"
     CDS             <1..792
                     /gene="hisA"
                     /locus_tag="VFV87_12940"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5104717.1"
                     /translation="VRYSRFSSNLNQRVEWVRAMQIWPAIDLRGGKCVRLKQGDYERE
                     TAYGDDPADMARRWIDQGADCLHLVDLDGARDGLQANLQAVESILAAVNVPCELGGGI
                     RDEAAIERMLGIGLARLVIGTRALKEPDWFRAMCRKYPGQLALGIDAKSGLVATDGWL
                     KTSTTPAIDLARQFAAEPLAAIIYTDIAKDGMLQGPNLDAMTEMNAAVDLDVIASGGV
                     TTAEDVRRLAEIGLAGCIIGRALYEGNLQLPAALSAAEATVARDS"
     gene            825..2921
                     /locus_tag="VFV87_12945"
     CDS             825..2921
                     /locus_tag="VFV87_12945"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5104718.1"
                     /translation="MSKHNVDDIRNIALCGHGGSGKTTLADKLLMQTGAVATEHSVDA
                     GTSILDFDPEEKAHKRTIESSICHFTHAGKRFNVIDTPGYADFIGQTIGALRGVDTAA
                     IVINAHSGIEVNTRRVFQEAGKAGIGRVIIINKMDEHNIDFPALIERIRDTFGTQCVL
                     VNVPIGHGPDFKGVVSTLGHAGNTAGALINPEEIHTSLIESIVEVDEELMMKYFEGEE
                     PPQEQLDSLIEHAVAHGTLVPIMCCSAKTGVGLTELLDLLVECAPPPTAVERKGSSDG
                     SEVEIKPDPAGPLVAQVFKTRIDPFVQKLSYIRVFSGTMKKDESVPTTQHKKGLKLGP
                     LLEVQGAETRPVDSASAGDIIAIAKMEDLHTGTTLGALELPKIPFPTPMVGLAVSPKS
                     RNDEAKLSASLHKIVEEDSTFKLDRDPQIKELVMTGMSELHLQIIRERLHRRDKLEVE
                     TKQPKIPFRETIQTTAEGSYRHKKQSGGRGQFGEVHIRMYPFPRGTKPEDFVTKDRFP
                     QMKDFHHDEPHNYLWINTVVGGSIPSNFLPAIEKGFMERMEKGVIAGCMVQDVAVEVH
                     FGKYHDVDSSEAAFKMAGSMCFRNVFQEARPALLEPIVKMEITVPEANVGDVYSDMSG
                     RGGRVQGSDNAGGGYQTVRCEIPLREVTTYSRTLSSMTGGQGSFTMDFSHYDVMPGNV
                     QQEIISKATLHEEEEE"
     gene            complement(2976..3632)
                     /locus_tag="VFV87_12950"
     CDS             complement(2976..3632)
                     /locus_tag="VFV87_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104719.1"
                     /translation="MNRLFVPLLTFASALTGSAFADDKPAAEGFVSLFDGKTHEGWQG
                     NKDGYAIIDGSFVCQPKGGGNLYTAKEYGDFHLKFEFKLTPGANNGIGLRTPLEGDPA
                     YVGMEIQVLDDSSEKYKGKLAEYQYHGSVYGIVPARQGHLKSVGEWNSEEIILKGKDI
                     KVILNGEAIVDANLDEVSKNGTIDKREHTGLKRDKGFICFCGHGAEVWFRNLRIKELK
                     "
     gene            complement(3730..>4684)
                     /locus_tag="VFV87_12955"
     CDS             complement(3730..>4684)
                     /locus_tag="VFV87_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104720.1"
                     /translation="GKHVYCEKPLSHTVPEARALAKLAAKNKLATQMGTQIHASDNYR
                     RVVELIQGGVIGKVTAVHNWCNKGWSDGRFVVSKNPPPKNLDWDLWLGPAKERPYCEK
                     IHPANWRRFWEYGSGTFGDMACHVMDLPFWALNLRHPVSVACEGPEVHPDGAPAWAKA
                     TYEFPGKDGQPLTMTWSDGGAHFDLVKETKDHDGKPLSSWGLGILFVGDKGMLAADYG
                     RRQLLPQDKFTDFKAPEQTIPNSIGHWHEWVQACKTGSATTCNFDYSGALAETVLLGI
                     VAFRSGEKLAWDAQNLRATNTPKAEEFLHKKYRKGFEVVGL"
BASE COUNT          928 a         1460 c         1437 g          859 t
ORIGIN      
        1 gtgcgatatt cgcggttctc atcgaatctt aaccagcggg tggagtgggt gcgggcgatg
       61 caaatctggc cggccattga ccttcgcggc ggcaagtgcg tgcgcctgaa gcagggcgat
      121 tacgagcgcg aaaccgccta tggagacgac ccagccgaca tggcccggcg ctggatcgac
      181 cagggggccg actgcctgca cctggtcgat ctcgacggtg cccgcgatgg cttgcaggcg
      241 aacttgcagg cagtcgaatc catcctggcc gccgtcaacg tgccgtgcga gctcggcggc
      301 ggcatccgag acgaagcggc catcgaacgg atgctcggca tcggcttggc ccgactcgtt
      361 attggcacgc gggccttgaa ggagcccgac tggttccgcg cgatgtgccg caagtatccc
      421 ggtcagctcg ccctgggcat cgacgccaaa agcggcctgg tcgccaccga cggctggctg
      481 aaaaccagca ccacgccggc gatcgacctg gcccggcaat tcgccgccga gccgctcgcc
      541 gcaatcatct acaccgatat cgccaaggac ggcatgctgc aggggccgaa cctggacgcg
      601 atgaccgaaa tgaacgccgc ggtcgatctg gacgtaatcg cctccggcgg tgtcaccacg
      661 gctgaagacg tccgccgcct ggccgagatc ggcttggccg gctgcatcat cggccgggcc
      721 ttgtacgaag ggaacttgca gttgccagca gcactttcgg cggccgaggc aacggtagct
      781 cgcgactcgt aaatcaaaac ctctcccaac aatagggtac gggcatgtcc aaacacaacg
      841 tggatgacat tcgcaacatt gctctgtgcg gtcacggtgg cagcggcaag accaccctgg
      901 ccgacaagct gctgatgcag accggcgccg tcgcgaccga acacagcgtc gatgccggca
      961 cgagcattct cgacttcgac cccgaagaga aagcccacaa gcggacgatc gagtcgagca
     1021 tctgccactt cacgcacgcc ggcaagcggt tcaacgtcat cgacacgccg gggtacgccg
     1081 acttcatcgg ccagacgatc ggcgccctcc gcggcgtcga tacggccgcc atcgtcatca
     1141 acgcccacag cggcatcgag gtaaacaccc gtcgcgtctt tcaggaggca ggcaaagccg
     1201 gcatcggccg tgtgatcatc atcaacaaga tggatgagca caacatcgac ttccccgcat
     1261 tgatcgagcg aattcgcgac acgttcggca cgcagtgcgt gctggtcaac gtgccgattg
     1321 gccacggccc cgatttcaaa ggggtcgtca gcacgctggg ccacgctggc aataccgccg
     1381 gcgcgctgat caacccggaa gaaatccaca cttcgctgat cgagtcgatc gtcgaagtcg
     1441 acgaagaact gatgatgaaa tacttcgagg gggaggagcc gccgcaggag cagctcgact
     1501 cgctcatcga gcatgcggtc gctcacggca cgctggttcc tatcatgtgc tgctcggcca
     1561 agacgggcgt ggggctgacg gaattgctag acctgctcgt cgagtgcgcg ccgccgccca
     1621 cggcggtcga gcgcaaaggt agcagcgatg gttccgaagt cgaaatcaaa ccggaccccg
     1681 ctggcccgct ggtggcccag gtcttcaaga cccgcatcga tccgttcgtg cagaagctga
     1741 gctatatccg cgttttcagc ggcacaatga agaaggacga atcggtcccc accacgcagc
     1801 acaaaaaggg gctcaaactt gggccgctgc tggaggtgca gggtgcggaa accaggccgg
     1861 tcgattccgc ttcggcgggc gatattatcg ccatcgccaa aatggaagat ctgcacaccg
     1921 gcacgacgct cggcgccctc gagctgccca aaatcccttt ccccacgccg atggtcggcc
     1981 tggccgtcag ccccaagagc cgcaacgacg aggcgaagct ctccgcctcg ctccataaga
     2041 tcgtcgagga agattcaacc ttcaagctcg accgcgatcc gcagatcaaa gagctggtga
     2101 tgaccggcat gagcgaactg cacctgcaga tcatccgcga gcgattgcac cgccgcgaca
     2161 agctcgaagt ggaaaccaag cagccgaaga ttccattccg cgagacgatc cagacgactg
     2221 ccgaaggaag ctaccgccac aagaagcaat cgggcggccg cgggcagttc ggcgaggtcc
     2281 acatccgcat gtatcccttc ccgcgcggga ccaagccgga ggacttcgtc acgaaggacc
     2341 gctttccgca gatgaaggat tttcatcacg acgagccgca caactacctc tggatcaaca
     2401 cggtcgtcgg cggctcgatt cccagcaact tcctgcccgc catcgaaaag ggcttcatgg
     2461 agcggatgga gaaaggcgtg atcgccggct gcatggtgca ggatgtggcg gtggaggtgc
     2521 attttggcaa gtaccacgac gtggacagct cggaagcggc gttcaaaatg gccggctcga
     2581 tgtgctttcg caatgtcttc caggaggccc ggcccgcgct gctggagccg atcgtgaaga
     2641 tggagattac ggttcccgag gccaacgtcg gcgacgtcta ttccgacatg tccggccgtg
     2701 gcggccgggt gcagggcagc gacaacgccg gcggcggcta tcaaacggtc cgttgcgaga
     2761 ttcccctgcg agaagtcacc acctattccc gcacgctctc cagcatgacc ggcggccaag
     2821 gcagcttcac catggacttc tcgcactacg atgtcatgcc cggcaacgtc cagcaggaga
     2881 tcatcagcaa agctacgctg cacgaggagg aggaagaata gcaattgaca accgaacgat
     2941 cggttctttt ccgcccgatc ggtcaaccga gagtgttact tcaactcctt aatcctcaaa
     3001 ttgcggaacc agacctccgc tccgtggccg cagaaacaga tgaatccctt gtcgcgcttc
     3061 aggccagtgt gctcgcgctt gtcgatcgtg ccattcttgc tgacctcgtc gaggttggca
     3121 tcaacgatcg cctcgccgtt caggatcacc ttgatgtcct tgcctttgag gatgatctct
     3181 tcactattcc actcaccgac cgatttgaga tgcccttgcc tggcgggcac gatgccatag
     3241 accgagccgt gatactggta ttcggcgagc ttgcctttgt atttctcgga cgaatcgtcg
     3301 aggacctgaa tctccatccc gacataggcc ggatcgccct cgagcggcgt ccgcaggccg
     3361 atgccgttgt tggcgcccgg cgtgagcttg aactcaaact tcaggtgaaa atcgccgtat
     3421 tccttggccg tgtacaggtt tccgccccct ttcggctggc agacgaacga gccgtcgata
     3481 atcgcgtagc cgtctttgtt cccctgccag ccttcgtgcg ttttgccgtc aaacaggctg
     3541 acgaatccct ccgcagcggg cttgtcatcg gcgaaggcgg atccagtcag ggcggaagca
     3601 aacgtgagca gcgggacgaa caagcggttc ataggaaggg tcctttatcg ggtaactgtt
     3661 tcgaggcact cggccattgt agtctccgga ccgatcgctc gtgcgggcta accgccaacc
     3721 tgcaatcgcc tacagcccga cgacctcgaa gcccttgcgg tatttcttgt ggaggaactc
     3781 ttcggctttg ggggtgttgg tggcgcggag gttttgggcg tcccaggcga gcttttcgcc
     3841 cgagcggaag gcaacgatgc cgaggaggac cgtttcggcg agggccccgg agtagtcgaa
     3901 gttgcaggtg gtcgcgctgc cggtcttgca ggcttgtacc cattcgtgcc agtggccgat
     3961 cgagttgggg atcgtttgct cgggggcttt gaaatcagtg aacttgtcct gtggcaggag
     4021 ctgccggcgg ccgtagtcgg cggcgagcat tcccttgtcg ccgacgaaca gaattcccag
     4081 gccccacgag ctgagcggct tgccgtcgtg gtctttggtt tccttgacga ggtcgaagtg
     4141 agcgccgccg tcggaccagg tcatcgtcag cggctggcca tccttgccgg ggaactcata
     4201 ggtcgccttg gcccaggccg gtgcgccgtc gggatgaacc tcggggcctt cgcaggcgac
     4261 tgaaactgga tgccgcaggt tcaaggccca gaacggcagg tccatcacgt ggcaagccat
     4321 gtcgccgaac gtgccagagc cgtattccca gaacctgcgc caattggcgg ggtggatttt
     4381 ctcgcaatat ggccgctcct tggccgggcc gagccacagg tcccagtcga ggttcttcgg
     4441 cgggggattt ttcgacacga cgaaacgccc gtcggaccag cccttgttgc accagttgtg
     4501 gaccgccgtc accttgccga tcaccccacc ctgaatcagc tcgacgaccc ggcggtagtt
     4561 gtccgacgcg tggatctgcg tccccatctg agtggccagc ttgttcttgg cggccaattt
     4621 cgccagagcg cgggcctcgg ggaccgtgtg cgagagcggt ttttcgcaat agacgtgctt
     4681 gccg
//
LOCUS       DASUGB010000678         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_771226, whole genome shotgun sequence.
ACCESSION   DASUGB010000678 DASUGB010000000
VERSION     DASUGB010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771226"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFV87_12960"
     CDS             complement(<1..505)
                     /locus_tag="VFV87_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5104721.1"
                     /translation="MKNILAVILAGGKGTRLEPLTRDRAKPAVPFGGLYRIIDFTLSN
                     CLNSDFRRVLLLTQYKAMSLDRHINMGWRHYFNRELGEFIDVVPPQQRIDEQWYQGTA
                     DAVYQNIYVLEKERPDYVVVLAGDHIYKMNYKSMVQYHLDVGADVTVGALRVDPVEAR
                     QFGVMEID"
     gene            complement(624..2594)
                     /gene="asnB"
                     /locus_tag="VFV87_12965"
     CDS             complement(624..2594)
                     /gene="asnB"
                     /locus_tag="VFV87_12965"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5104722.1"
                     /translation="MCGITGAIWTDPAKAIDAGVLARMTAALRHRGPDDEGQYYSQFR
                     LRPPYEAMPGVALGFRRLAIIDLATGNQPMSNEDGSVWMVFNGEIYNFQDLRHRLEGS
                     GHRFRTESDSETIIHLYEDEGPQCFSYLNGMFSVAIWDANRRRLVLGRDRLGKKPLVY
                     RQESGRMLFASELKSLLEVPGLPREIDPAAVDEYLTYQFVPHPNCILKGYRKLPPGHF
                     AVWQDDKLEVKPYWQPDFTAERRLPKATAISQLRELLESAVKLRLRSDVPLGAFLSGG
                     IDSSLIVALMQKHSAEPVKTFTIGFPVKEYDETKYAELVARHLGTEHHTLQVTPSAVE
                     ILPKLAWHYDEPFGDSSAIPTWYVSKLTREHVTVALSGDGGDELFAGYPRYRAAALGA
                     RLDWLSPVKSVLAARAWQWLPTSGRQKSKLRQFKRFSEALSQSPERRYLDWISIFNER
                     RRADLYQDDFLAQLSTDPAAFLRSAWKRCAGRDAASCASLADLVTYLPCDLMTKVDIA
                     SMAHGLECRAPFLDYRVAEFAAALPARYKLRGGRGKRILRDAFGDLLPREIWTRKKMG
                     FGVPLDDWFRHELKELTTDLLLSDNSRAREFLRPESVQLLWDEHQARRFDHSARLWAL
                     VMLESWLREWTQARRASEAVREQTDSLADASG"
     gene            complement(2618..>3116)
                     /locus_tag="VFV87_12970"
     CDS             complement(2618..>3116)
                     /locus_tag="VFV87_12970"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX5104723.1"
                     /translation="IVRTRYLTDVDNLRRLGADEIIPEELETSIEIFARVLDRYGVPR
                     NLILDLVDRLRRDHYGILRDLGHSPTKMELPLDVISKLDIELCALRSEAPAIGKSLAE
                     LNLRAQTGATVIGVRRGKEILANPGPDFHFAAGDIALILGDRPQLDRALCALDPTLED
                     SNPDS"
BASE COUNT          562 a          993 c         1001 g          560 t
ORIGIN      
        1 cgtcgatctc catcacgccg aattggcgag cctcaaccgg atcgacccgc agcgcgccga
       61 cggtcacgtc cgcgccgaca tccagatggt actggaccat cgacttgtag ttcatcttgt
      121 aaatgtggtc gccggcgaga acgacgacgt agtcgggccg ctccttttcc aggacgtaga
      181 tgttctgata cacggcgtcg gcggtcccct ggtaccactg ctcgtcgatc cgctgctggg
      241 gcgggacgac gtcgatgaat tcccccagct cgcggttgaa atagtgccgc cagcccatgt
      301 tgatgtggcg gtcgaggctc atcgccttgt actgcgtgag caagagcacg cggcgaaaat
      361 cgctgttcag gcagttcgac agcgtgaaat cgatgatgcg atacaaaccg ccgaacggca
      421 ccgccggctt ggcccggtcg cgcgtcagcg gctccagccg cgtccctttc ccgcccgcga
      481 ggatgacggc caggatgttc ttcatgaggt gctccgtgcg ggttgtatcc gtggtgtgcg
      541 atcgcgtttc gctcgatttt agcgggaatc gaggggcggc ccaaccgggc gacactaacc
      601 cgacgcgcca gcgagggagc atcctaaccc gaagcgtcag cgagggagtc cgtctgctcg
      661 cgcactgcct cgcttgcgcg tcgcgcttgg gtccactcgc ggagccacga ttcgagcatg
      721 accagcgccc agaggcgagc gctatggtcg aagcggcggg cctgatgctc gtcccacagg
      781 agctgcacgc tctcggggcg caggaactcg cgcgcgcggg agttatcgga cagcaacagg
      841 tcggtggtca gctccttcag ctcgtggcgg aaccaatcgt cgagcggcac gccaaagccc
      901 atcttcttcc gcgtccagat ttcccgcggc aagaggtcgc cgaaggcgtc gcgcaagatg
      961 cgcttgccgc ggccgccgcg aagcttgtaa cgggcgggca aggcagccgc aaactcggcg
     1021 acgcgatagt cgagaaacgg tgctcggcac tccaagccat gcgccatcga cgcaatatcg
     1081 accttcgtca tcaggtcgca cggcagatag gtcaccaggt ctgccagcga agcgcagctc
     1141 gccgcgtcgc ggccggcaca gcgtttccag gcggaccgca gaaaggccgc gggatcggtc
     1201 gaaagctggg ccagaaaatc gtcctgatat aaatccgccc ggcgacgctc attgaagatc
     1261 gagatccagt cgagataccg ccgctcgggc gactgggaaa gcgcctcgct gaaccgcttg
     1321 aactgccgca gcttcgattt ctgccggccc gacgtcggca gccactgcca cgcgcgggcc
     1381 gccagtactg acttgaccgg cgagagccag tcgagccgcg ctccgagcgc cgccgcccga
     1441 tatcgcggat aaccggcaaa cagctcgtcg ccgccgtcgc cgctcaaagc caccgtgaca
     1501 tgctcgcgcg tcaacttcga gacataccag gtcggaatcg ccgagctgtc gccgaaaggc
     1561 tcgtcgtagt gccaggcgag cttgggcaga atctcgacgg cgctgggcgt cacttgcagc
     1621 gtgtgatgtt cggtccccag gtgcctggca accagctccg cgtacttcgt ctcgtcgtac
     1681 tcctttactg gaaagccaat cgtgaacgtc ttcaccggct ccgcggagtg tttctgcatc
     1741 agggcgacga tcagcgacga atcgatcccg ccgctgagga acgccccgag cggcacatcg
     1801 cttcgcaagc gcagcttgac cgccgattcg agtagttcgc gaagctggct gatcgccgtc
     1861 gccttcggca gccggcgctc ggccgtaaag tctggctgcc agtagggctt cacttccagc
     1921 ttgtcgtctt gccatacggc gaaatgcccc ggcggcagct tgcgatagcc cttgaggatg
     1981 cagttggggt gcgggacgaa ttgataggtc aaatattcat cgaccgcggc ggggtcgatt
     2041 tcccgcggca gccccggcac ttccagcagg ctcttcagct cgctggcgaa aagcatccgg
     2101 ccgctttcct ggcgataaac gagcggcttt ttgcccagcc ggtcgcgtcc cagcaccagg
     2161 cgccggcgat tggcgtccca gattgccacc gaaaacatgc cgttgaggta gctgaagcac
     2221 tgcgggcctt cgtcctcgta gaggtgaatg atcgtctcgc tgtcgctttc ggtccgaaag
     2281 cgatgccccg agccctccag ccggtgccgc aggtcctgga agttgtaaat ctcgccgttg
     2341 aagaccatcc acacgctgcc gtcttcattg ctcatcggct gattgccggt ggccaggtcg
     2401 ataatcgcca gccgccgaaa gccgagcgcg acgccgggca tcgcctcata tggcggccgc
     2461 aggcggaact ggctgtaata ttgcccctcg tcgtcgggcc cgcggtggcg gagcgctgcg
     2521 gtcatccgcg cgagcacgcc ggcatcgatc gccttcgcag gatcagtcca gattgcgccg
     2581 gtgatgccgc acatggggag gggtcagggg tcaagagtca ggaatcaggg tttgagtctt
     2641 cgagcgtcgg atcgagggcg cacagcgcgc ggtcgagctg cgggcggtcg cccaggatga
     2701 gggcgatgtc gcccgcggcg aagtggaagt cggggccggg gttggccagg atttccttgc
     2761 cgcggcggac gccgatgacg gtcgctcccg tctgcgcccg caaattcagc tcggccagcg
     2821 acttaccgat ggccggcgcc tcgctgcgca gcgcgcacag ctcaatatcg agtttcgata
     2881 ttacatccaa aggcagttcc atcttcgttg gtgagtgccc caggtcgcgg agaattccat
     2941 aatgatcgcg ccgcaaacga tcgaccagat cgaggatgag gttccgtggc acgccgtagc
     3001 ggtcgaggac gcgggcgaag atctcgatcg aagtctccag ttcctcgggg atgatctcgt
     3061 cggcccccag gcggcgaaga ttgtccacgt cggtcagata gcgcgttcgc acgatg
//
LOCUS       DASUGB010000679         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1326506, whole genome shotgun sequence.
ACCESSION   DASUGB010000679 DASUGB010000000
VERSION     DASUGB010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326506"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..266)
                     /locus_tag="VFV87_12975"
     CDS             complement(72..266)
                     /locus_tag="VFV87_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104724.1"
                     /translation="MNEKILGAIRQRARAAVSNEDLVRAVFDSFRSQSIDLRHVSLEE
                     MKAALLEAARAAREPAVVSA"
     gene            complement(592..849)
                     /locus_tag="VFV87_12980"
     CDS             complement(592..849)
                     /locus_tag="VFV87_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104725.1"
                     /translation="MNAPADKNQPRRPPFQFSLMGLMVLMFAISAAAAPGYYMFRSGM
                     QEGPSTRDSWLIGMLMMLAGPLLIMTLLSIALSLFGRGKRQ"
     gene            complement(846..1763)
                     /locus_tag="VFV87_12985"
     CDS             complement(846..1763)
                     /locus_tag="VFV87_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5104726.1"
                     /translation="MCAPSQTAAEVAAACRTAAEANRACPSRRGNVIELSPDNAGDVM
                     IVADLHGNRLNFEKLLRIADLESHPRRHLIMQEVCHGGPEYPGEGGGCMSHLLLEDCI
                     RLKNEHPERFHFLLSNHELAELSDYPICKSRRMLNLVFRAGISEMYGAAAEEVRNAYL
                     EFLSTCPLAVRLTSGVFISHSLPDECDREPFDVSVLQRPLGCADFAAHSPAFKLVWGR
                     DFRAENADAFARQVGADVLIHGHEPCEAGFSAPNKRQVILDGCCSQASYLILPVGPKL
                     THADVVARVASLHRSACVPGPCTPVPEVV"
     gene            2022..2459
                     /locus_tag="VFV87_12990"
     CDS             2022..2459
                     /locus_tag="VFV87_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104727.1"
                     /translation="MEADHRKSFRIVSPEGQDRAALRVGLRTYNVRVTDFSAGGYALA
                     CSEALPVCRGDLLRLRTSGGWQEVQVVRQERFSDGVLLGVERVREIEDPNPAGLVRSG
                     WFDWFFPPDTMDPHGNFSGNTLRVAMLVGALLIAVVWLVVNYR"
BASE COUNT          480 a          803 c          813 g          468 t
ORIGIN      
        1 gggttcccaa gcaggagctt gggaaccaga gcgatccgtc gcgcgcagtg ggcggaccgc
       61 taaaccacga gtcaggccga aaccactgcg ggctcgcggg cggcgcgggc cgcttccagc
      121 agggcggcct tcatctcttc cagcgagacg tgacgcaggt cgatcgactg agaccggaac
      181 gaatcgaaca ccgcgcggac caggtcttcg ttgcttaccg ccgcgcgggc gcgctggcgg
      241 attgctccta atattttctc attcattttg ctttcttcct tgaatggatt cgcgtagctc
      301 atcaagtgcg acgattccga ccgccgcctg gccttacaaa aacgtagctt gcgggccaaa
      361 aaacgcagat gttgcaatcc gtgtgccccg ggcgtgggca ctgtgggtcg gcactgtgcg
      421 tcggcgacca cgctggcatt cataagactg acacgtctcg gcagttgcgg ttcgcaagga
      481 ataaaagttc tgtcccggcg ggaattctct tgactcggac cgcccgaaaa gtgcctgccg
      541 aattggcaat aagtgctgca accggccgca gtcgccggct actgtcgccg gctactgtcg
      601 cttgccgcgg ccaaacagcg agagggcaat gctcagcagc gtcataatca agagcgggcc
      661 agccagcatc attagcatcc cgatcagcca cgaatcgcgc gtgctggggc cctcttgcat
      721 tcccgagcga aacatatagt agcccggcgc agccgccgcc gagatggcga acatcagcac
      781 catcagcccc atcaggctga attgaaacgg cggacgccgc ggctggttct tatcggcagg
      841 tgcgttcaca cgacttcagg cactggcgtg caaggcccgg gcacacaagc ggaacggtgc
      901 aagctggcga cgcgggcgac gacatccgca tgcgtcagct tcggccccac cggcaggatc
      961 aagtacgacg cctgcgaaca gcagccgtcg agaatcacct gccgcttgtt cggcgcgcta
     1021 aagcccgctt cgcacggctc gtggccgtgg atcagcacgt cggcgccgac ctggcgggcg
     1081 aaggcatcgg cattttcggc gcgaaaatcg cgaccccaca cgagcttgaa cgccgggctg
     1141 tgcgccgcaa aatcggcgca gcccagcggg cgctggagca ccgagacatc gaacggctcg
     1201 cggtcgcatt cgtcgggcag gctatggctg atgaacacgc cgctcgtcag gcgcaccgcc
     1261 aaggggcagg ttgagaggaa ctccaaatac gcattgcgga cctcctcggc cgcggcgcca
     1321 tacatctcgc tgattccggc gcggaacacg aggttgagca tccgccgcga tttgcagatt
     1381 gggtaatcgc tcagctcggc cagctcgtgg ttgctgagga ggaagtggaa tcgctcggga
     1441 tgctcgttct tcaggcggat gcagtcctcc aacagcaaat gcgacatgca gccgccaccc
     1501 tcgcccggat attccggccc gccgtgacag acctcctgca tgatcagatg ccgccgcgga
     1561 tggctttcca ggtcggcaat ccgcaggagc ttctcgaagt tcagccggtt gccgtgcagg
     1621 tcggcgacga tcatcacgtc gccagcgtta tcgggcgaaa gctcgatgac gtttccgcgc
     1681 cgcgaagggc aggcccggtt tgcttcggcg gcggtacggc aagcggcggc gacttcggcg
     1741 gcggtctgtg agggagcgca catcaagcaa tccatcaagc cgctttggcc cgtgtttcgt
     1801 tgtaaactcg cgatcccgcg ctggaaggag cgcctgggat ccccaatagc cgctggtggt
     1861 aagtatatgc gccggcaggg gctcggacca accggtgctg cccgaccgaa ataacccgaa
     1921 atccagtctc cacccctata gaccgcctaa accggccagc gacctgaaag aaaatcgcaa
     1981 tgtaggcttc atctagtgtt gtttcgcgtc aaaggcggtc tatggaagcc gaccatcgta
     2041 agtcgtttcg catcgtctcg cccgaagggc aggaccgggc cgctttgcgg gttggcctgc
     2101 ggacgtacaa cgtgcgcgtc accgactttt ccgccggcgg ctatgcgctg gcatgcagcg
     2161 aggcgcttcc cgtctgccgg ggcgatctgc tgcgactgcg gacctctggc ggctggcagg
     2221 aggtgcaggt tgttcgtcag gaacgcttct cggatggtgt cctgctgggc gtcgagcggg
     2281 tacgcgaaat tgaagatccg aatcccgcgg gactcgtccg ctcaggctgg ttcgattggt
     2341 tctttccgcc ggacaccatg gacccgcacg ggaatttctc cggcaacacg ctgcgggtcg
     2401 ccatgctggt tggcgccctg ctgatcgcgg tggtttggct ggtcgtgaac taccgctagg
     2461 ccaccgtagg ctgctcgctc ccgcgagtag cggacagcct tccatgcgcg agggcggttg
     2521 cacattgcca cgtccatcgc ttctcgcgga gcgagaagcc tacg
//
LOCUS       DASUGB010000680         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1881827, whole genome shotgun sequence.
ACCESSION   DASUGB010000680 DASUGB010000000
VERSION     DASUGB010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881827"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFV87_12995"
     CDS             <1..1502
                     /locus_tag="VFV87_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5104728.1"
                     /translation="GAKSWLCHSKVDRHQPILPWNAPADVPKVSPVVASRRYLEHLVA
                     AWQHAHPEASIAEQLVVLTVPASFDAAARELTREAALAAGLPANLILLEEPQAAVYAW
                     LTTTGERWRRILKVGETLLVCDVGGGTTDLTLVGVHQEAGELSLRRIAVGEHLLVGGD
                     NMDLALAHFVAGKFGEKGVKLDPWQSVSLWHQCRSAKESLLAEGGPKKHPLSVLGRGS
                     KLIGGTVKVEIERDAASNLLLEGFFPPCAAADRPARRRQSGFQEIGLPFESDTAVTRH
                     LAAFLSAHGSGDQAVTPSHVLFNGGVFKADAFRNRLMETLTKWSSETPPQVLAGEHDL
                     DHAVARGAAYYGWAKQHGGVRIRGGTARSYYVGIESSGLAVPGAPRPLRALCVVPFGM
                     EEGTSVDVPSGEFGLVVGEPATFRFFSSAVRKQDQPGELLPSWSDEELAESDSLEALL
                     PPDDTIEDDFVPVRFQSHITELGVFELWCQSTKSSNRWKLEFSVRKDAE"
     gene            1509..>3230
                     /locus_tag="VFV87_13000"
     CDS             1509..>3230
                     /locus_tag="VFV87_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5104729.1"
                     /translation="MSADLLAEERPSRYVVGIDLGTTNSAVCYVDTHERPWRVRVLEI
                     PQLVAPGQVEGRDTLPSFHFQPLQTLEPRTPNPEPRFVVGFMARDYGAASPGRLISSA
                     KSWLCHTGVDRTADLLPWHAAADVERISPVEASSRYLAQIRTAWNGRFKSDPLEQQDI
                     VLTLPASFDEIARELTIQAAARAGLPKVVLIEEPQAAFYAWIYKHQANWEQLVSPGQK
                     ILVCDIGGGTTDFTLIRVRTSDEGKVQFHRVAVGDHLILGGDNMDLTLAKNLEEKLGG
                     QLPPRHWDVLLRLARQAKEQLLSDEAPEEIKITLPGSGSKLIGGALQAKVTRQQAHEL
                     LVEGFMPRVALTDKPAARRSGFQEFGLPYAADAAITRHLAAFLTAHRHAGEEGDRGQG
                     TGDRGGDSARPDVVLFNGGVFESALLRKRLIDCLTDWFPAGSRTPNPEPLAPQVLDND
                     RLDLAVARGAAYYGMVRRGEGVKIVASLARTYYIGVESRNATEGAPDSAEPRAPNPEP
                     RAPFALCLVPGSAEPGQEIELTERQFDLLVSEPVEFPLYTSSIRLTDQPGELVAVDPE
                     QMTALPPI"
BASE COUNT          550 a         1027 c         1083 g          570 t
ORIGIN      
        1 tcggcgccaa gtcttggctc tgccacagca aggttgatcg ccatcagccg atcttgccct
       61 ggaacgcacc ggcagacgtg cccaaagttt cgccggtcgt ggcctcgcgg cgctatttgg
      121 agcacctggt cgcggcctgg cagcacgctc atcccgaggc gtcgatcgcc gagcagctcg
      181 tcgttttgac cgtgccggct tcgttcgacg ctgcggcccg cgagctgacg cgcgaagccg
      241 ccttggcagc cggcttgccc gctaacctga tcctgctcga agagccgcag gcggcggtct
      301 atgcgtggct gacgacgacg ggcgagcgtt ggcggcgaat tctgaaagtc ggcgagacgc
      361 tgctggtgtg cgatgtgggc ggcggaacga ccgacttgac gctggtcggc gtccatcagg
      421 aagcgggaga gctgtcgttg cggcggatcg cggttggcga gcatctgctc gtcggcggcg
      481 acaatatgga tctggccctg gcccacttcg tggccggcaa gttcggtgag aaaggggtca
      541 agctcgatcc gtggcaatcg gtttcgctgt ggcaccagtg tcgctccgcg aaggaatcgc
      601 tcttagccga aggcgggccg aagaagcatc ccctcagcgt tctgggccgc ggcagcaagc
      661 tgatcggcgg gaccgtgaag gtggaaatcg agcgcgatgc cgcgtcgaac ctgctgctgg
      721 agggcttttt tccgccgtgt gcggccgcgg atcgtccggc acgccgccgg caatcgggtt
      781 ttcaggaaat cggcctgccg tttgagtcgg ataccgccgt gacacggcat ctggcggcgt
      841 tcttgtccgc tcatggcagt ggtgaccaag cggtcactcc ctcgcatgtc ctgttcaatg
      901 gcggcgtgtt taaagcagat gcgttccgca accggctgat ggaaacgctg acgaagtggt
      961 cgagtgaaac gccgccgcaa gtgctggccg gcgagcacga cctggatcac gccgtggccc
     1021 gcggtgcggc ctactatggc tgggcgaagc agcacggcgg cgtgcgaatt cgcggcggca
     1081 cggcccggtc gtactacgtt ggcatcgagt cgtccggact ggcggttccc ggcgcgccgc
     1141 ggccgctgcg ggccttgtgc gtcgtgccgt ttggcatgga ggaagggacg agcgtcgatg
     1201 ttccatcggg cgagttcggc ctggtcgtcg gcgagccggc gacgttccgc ttcttcagct
     1261 ccgcggttcg caaacaggac caacccggcg agctcttgcc gagctggtcc gacgaagagc
     1321 tggcagaatc cgattcgctg gaagccctgc tgccgcccga cgacacgatc gaagacgact
     1381 tcgtcccggt gcggttccag tcgcacatca ccgagctcgg cgtcttcgag ctgtggtgcc
     1441 agagcaccaa gtcgtcgaat cgttggaagc tggaattcag cgtgcgcaaa gacgcggagt
     1501 agggccgcat gtccgccgac ctgctcgccg aagaacgccc cagccgctat gtcgtcggca
     1561 tcgacctcgg aacgacgaat tcggcagtct gctatgtcga tacgcacgag cggccgtggc
     1621 gggtgcgggt gctggagatt ccgcagctcg tcgcgccggg acaggtcgag ggccgggaca
     1681 cgctgccgag ttttcatttc cagcccttgc agacactcga gccccgaacc ccgaaccccg
     1741 aaccccgttt cgtcgtcggc ttcatggccc gcgactacgg cgcggcttca cctggccggc
     1801 tgatttcgtc ggccaagtcg tggctgtgcc acacgggggt cgatcgtacg gccgacctgc
     1861 tgccgtggca cgcggctgcg gatgtcgagc ggatttcgcc ggtcgaagcc agcagccgtt
     1921 atctggccca aatccgcacg gcctggaacg gcagattcaa atccgatccg ctggagcagc
     1981 aggacatcgt cctcacgctg cctgcgtcgt tcgacgaaat cgcccgcgaa ttgaccatcc
     2041 aggccgcggc acgagccggc ttgccgaaag tcgtcctgat cgaagagccg caggcggcat
     2101 tttatgcctg gatttacaag caccaggcga actgggagca gcttgtttca ccggggcaga
     2161 agatcctcgt ctgtgacatc ggcggtggca cgaccgactt cacgctgatt cgcgtccgca
     2221 cgagtgacga aggcaaagtg cagttccacc gcgtcgcggt cggcgatcac ctgatcctcg
     2281 gcggcgacaa catggatttg acgctggcga agaatctgga agagaaactg ggtggtcagc
     2341 ttccgccgcg ccactgggat gtgctgctcc gcctcgcccg gcaggcgaaa gagcagttgc
     2401 tgagcgatga agccccggag gaaatcaaga tcacgcttcc cggcagcggg tcgaaattga
     2461 tcggcggagc gctccaggcg aaagtcacgc gccaacaagc ccacgagctg ctcgtggagg
     2521 gcttcatgcc ccgcgtcgcc ttgaccgaca aaccggccgc ccgccgcagc ggctttcagg
     2581 agttcggcct gccgtatgcc gcagatgcgg cgatcacgcg gcatttggcg gcgtttttga
     2641 cggcacatcg gcacgcagga gaagaagggg acaggggaca gggaacaggg gataggggcg
     2701 gagattcggc gcggccggat gtggtgttgt tcaatggcgg cgtgtttgag tcggccctac
     2761 ttcgtaagcg gctcatcgat tgcctgacag attggtttcc tgccggctcc cgaaccccga
     2821 atcccgaacc cctagccccg caggttcttg acaacgaccg cctggacctc gcggtcgccc
     2881 gcggcgcggc ctactacggc atggtccgcc gcggcgaagg ggtgaagatc gtcgcttcgc
     2941 tggccaggac ctactacatc ggcgtcgaat cgcggaacgc cacggagggc gctcccgaca
     3001 gcgccgaacc ccgagcccca aaccccgagc cccgagcccc cttcgccctc tgcctcgtcc
     3061 ccggcagcgc ggagccaggg caggagatcg agctcaccga gcggcagttc gatctgctcg
     3121 tctccgagcc ggtcgagttt ccgctttaca cgtcgagcat ccgtttgacc gatcagccgg
     3181 gcgagctggt cgcggtcgat cccgagcaga tgaccgccct gccgccgatt
//
LOCUS       DASUGB010000681         4116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_494091, whole genome shotgun sequence.
ACCESSION   DASUGB010000681 DASUGB010000000
VERSION     DASUGB010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494091"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VFV87_13005"
     CDS             <1..1485
                     /locus_tag="VFV87_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104730.1"
                     /translation="APTSLKIEYQDAVTSGNWNSVNATGGNTASGQSQGQVTFRPMVS
                     SRSINLRAEIADAGGNVAYFSQRMSLVPVRPAPSSGAAFAPPADPSATPWPSDARASS
                     ARPNQWIASETTGAVAPIRSSRNEGSEQVRIPSVVQNPYSTPAKMASSPSGANPVAEE
                     VLPPPQPMTSRSEVYPGNGFEMSSPPPSDSAADHRAFAPSVPTAQDYQSSVQPPAESQ
                     DEYGPLLRHDSQYGTTSDVPSDAPAASDFNVAPEVMPSPEPTQPSAGETVEAPYGQRP
                     RLTNSRRFSLDYDVESVGPEGVAAVELWGTTDGGRTWGKWGVDPDRASPFDVEVNNEA
                     TYGFKIVIVGRNGLATSSPQPGDAADIWVNIDGTRPAARLTGASYGQSQTAGKLDIRW
                     EAADANLGPRPITLAIGDRPDGPFAPIAAGLPNTGQYFWEYDPRSPRQMYLRLEVRDE
                     AGNVSIDQLTEPIKVEGLEPKGRIRGFNPGPETTREAFRSPLFQ"
     gene            complement(1722..2222)
                     /locus_tag="VFV87_13010"
     CDS             complement(1722..2222)
                     /locus_tag="VFV87_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEX5104731.1"
                     /translation="MTSPRDTYLETQITTATPQRLRLMLIEAAIREAKRTAEHWRINH
                     NDEALETLIRCRAIVGELIAGVSDAQSPLARRVLGLYGFLFTSLTEAQLTRDAARLDA
                     VVRILEEESQTWRQVCTSPPDLPPQRQTPGHSEETAPSRVASGWPGAYDSRGTLDSSP
                     GLSLDA"
     gene            complement(2335..>4116)
                     /gene="fliD"
                     /locus_tag="VFV87_13015"
     CDS             complement(2335..>4116)
                     /gene="fliD"
                     /locus_tag="VFV87_13015"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX5104732.1"
                     /translation="ADGKRIELTDLTTDNGGTFAVSNSVGGSTAAELGLVGPASGDSI
                     SGRRLISGLKTTLLGTLGGGNGLGTLGQLSLTDRSGASATVNLAAAETLDDVIDAINA
                     AGLGIKAQYNSARNGLELVDTTGATASHLIAADADATNTATRLGLAANVDANRINSGS
                     LDRQVVSRSTLLSAYNGGKGVSSGSFLITNSNGQQSAVNLTILKPQTVGDVIDAINSL
                     NAGVTASINASGDGILLTDTAGGSGKLTVSDVGAGRAAQDLHLAGAAEATTIDGSTTV
                     TISLEADETLDDLVAKINDLGAGVTASVFSDQSGSLRHHLSLLSGIAGQAGELVIDGS
                     GLGLTFRDIAPAQDALLQIGSGGAFGGQLVTSTTNQFHDILPGLDVTLHGQSPDPVTL
                     SVNTTADSAAGAIQTFVDQYNKLREKLDKYTAFNETDGSKGILFGSSETLRLDAELAR
                     LVTGRYSGLGDVQSLAELGVSIDDQGKLSFDKSKLQARYDADPESVQKFFTDEDRGFG
                     AKVDALIETLAGRDRSALVTRAQALQRQVETAEERVTRMTERLDRSRERLLLQFYRME
                     EAVARVQNQLTALSQLQILEPLQFTNQ"
BASE COUNT          709 a         1348 c         1302 g          757 t
ORIGIN      
        1 gctccgacgt cgctgaagat cgagtaccag gacgccgtga ccagcggcaa ctggaactcg
       61 gtgaacgcaa ccggcggcaa tacggccagc ggccagtcgc aagggcaggt aaccttccga
      121 ccgatggtct caagccgctc gatcaatctg cgggccgaga tcgccgatgc cggcggcaat
      181 gtggcctatt tcagccagcg catgtcgctc gtgcctgttc gacccgcgcc aagcagcggc
      241 gcggccttcg ctccgcctgc cgatccttcg gcaaccccct ggcccagcga cgctcgcgcc
      301 agcagcgcca ggccgaacca gtggatcgcc agtgagacga ccggcgccgt agcgccaatc
      361 cgttcgagtc gtaacgaagg cagcgagcag gtgcggattc caagcgtcgt ccagaatccg
      421 tattcgacgc cggcgaaaat ggcaagttct ccatcgggag ccaatccagt tgccgaagaa
      481 gttttgccgc cgccacagcc gatgacgagc cgttccgagg tctatcccgg caacggattt
      541 gagatgtcga gtccgcctcc ttccgattcg gcggccgacc atcgggcgtt tgccccctcc
      601 gtgccgacgg ctcaagacta ccaatcttcg gtacagccgc cggccgaatc tcaggacgag
      661 tacgggccgc ttttgcgaca tgacagccaa tacggcacta cttcagacgt gccttcggac
      721 gcacctgcgg caagtgattt caatgtcgcg ccggaagtca tgccctcgcc ggagccgacg
      781 cagccgtccg cgggcgaaac cgtcgaagcg ccgtacggcc agcgcccgag gctgaccaac
      841 tcgcggcgct tcagcctcga ctatgacgtc gaatcggttg ggccggaggg cgtcgctgcg
      901 gttgagctgt ggggcacgac cgacggcggc cgcacctggg gcaaatgggg cgtcgatccc
      961 gatcgggcca gtccgtttga tgtcgaagta aacaacgaag cgacatacgg tttcaaaatc
     1021 gtaatcgttg gccgcaatgg cctggccacc agcagcccgc agccaggcga cgccgccgac
     1081 atttgggtca acatcgatgg cacgcgtccc gcggcacgcc tgacgggcgc ctcgtacggc
     1141 cagagccaaa ctgccggcaa gctcgacatc cgctgggaag cggccgacgc caatctcggc
     1201 ccgcggccca tcacgctagc catcggcgac cgccccgacg gcccgttcgc gcccatcgcc
     1261 gccggcctgc cgaacaccgg ccagtatttc tgggagtacg atccgcgcag cccgcggcag
     1321 atgtacttgc ggctggaagt ccgcgacgaa gcgggcaacg tctccatcga ccagctcact
     1381 gagcccatca aggtcgaagg cctcgagccc aagggccgca tccgcggctt caaccccggc
     1441 cccgagacca cccgcgaagc cttccgcagc ccgctgttcc aataggctgg cttcacccgc
     1501 cgcgcacgcg tgcgcctccg cctcgatcca cccgcacgat ttgggagtgc tcgtcggttt
     1561 gtttcacggg acagctgcaa agccaagcac ttgctttgtc catatcccgt tgaattcgtg
     1621 gactaagtac ggcttgggtc tacgaacctc actaagaacc tctaacacaa cccactgcta
     1681 gctcggaatt ctccgaggat ttcgcggttt cgttttagct tctaagcatc aagagacaga
     1741 ccgggcgacg aatccaacgt gccccgagag tcgtacgcgc ctggccatcc gctggccacg
     1801 cgagatggcg ccgtttcctc gctgtggccg ggcgtctgtc gctgcggcgg caagtcggga
     1861 gggctcgtgc agacctgacg ccaggtctgt gattcctctt ccagaatccg cacgaccgcg
     1921 tcgaggcggg ccgcgtcacg cgtaagctgg gcctcggtca gcgaagtgaa tagaaacccg
     1981 tacaagccca gtacgcggcg cgcgagcggc gactgagcat cgctcacgcc cgcaatcagc
     2041 tcgccgacaa tggcccgaca gcggatcagc gtttccaagg cctcatcgtt gtggttgatt
     2101 cgccaatgtt cggccgtccg tttagcttcg cggattgccg cctcgatcag catcaaccgc
     2161 aggcgctgcg gcgtagccgt cgtaatttgg gtttccaggt aggtatcgcg gggggaggtc
     2221 atggagcagg tgagtaagtg ttggctcgat ttgggattgc ggatttcggg tgaggatggg
     2281 tgtcgatgtg ccgtggctgc cggctcctgc ctcctggccc ctggctcctg gcccctactg
     2341 gttcgtgaac tgcagcggct cgagaatctg gagctgactg agggccgtga gctgattttg
     2401 aacgcgagcg acggcttcct ccatgcgata gaattgcagc agcaatcgct cgcggctgcg
     2461 atccagacgc tccgtcattc tcgtaactcg ttcctcggcc gtttcgacct gccgctgcag
     2521 cgcctgagcc cgggtgacaa gcgccgaacg atcacgcccg gccagggtct cgatcagggc
     2581 atcgactttc gcgccgaatc cgcgatcctc gtcggtgaag aatttctgga cggactcggg
     2641 atcggcgtca taacgggcct gcaacttcga cttatcaaac gaaagcttgc cctggtcgtc
     2701 gatcgagacg cccagctcgg ccaacgactg cacgtcgccc aggccagagt atcgcccggt
     2761 caccagcctt gcgagctccg catccagccg caaggtttcc gacgagccga acagaatgcc
     2821 cttgctgccg tcggtttcat tgaacgccgt gtacttgtca agcttctcgc ggagcttgtt
     2881 gtactgatcg acgaaggtct ggatggcccc ggcggccgaa tcggctgtcg tattcacgga
     2941 caaggtcacc gggtcgggcg actggccgtg cagcgtcacg tccagacctg gcagaatgtc
     3001 gtggaactgg ttggtggtcg aagtaacgag ctgcccgcca aacgccccgc cggagccgat
     3061 ctgcaggagt gcgtcctggg cgggcgcgat gtcgcggaac gtcaggccca gccctgagcc
     3121 gtcgatgacg agttcgcccg cttggcccgc gattccgctg agcagcgaga ggtgatgccg
     3181 cagcgatccg ctctggtcgc tgaagacgct agctgtgacg cccgctccga ggtcattgat
     3241 cttggcgacg aggtcgtcga gcgtctcgtc ggcctccagc gagatcgtaa cggtcgtgct
     3301 tccgtcgatc gtcgttgcct cggctgctcc ggccaggtgc aagtcttgag cagcccgccc
     3361 tgcgcccacg tcgctgacgg tcagcttgcc gctgccgccg gccgtatcgg tcagcaggat
     3421 gccgtcgccg gaggcgttga tgctggcggt cacgccggcg ttcaggctgt tgatcgcatc
     3481 gatgacgtcg cccaccgtct ggggcttgag aatggtgaga ttgacggccg actgctgccc
     3541 gttggaattg gtgatcaaga acgagccgct gctgactcct tttccaccgt tgtacgcgga
     3601 gaggagcgtg ctgcggctga cgacctgccg gtcgagcgag ccgctattaa ttcgattggc
     3661 atcgacgttg gccgccaggc cgagcctggt agcggtattg gtcgcgtcgg catcggcggc
     3721 gatgaggtgg ctggccgtgg cgcccgtcgt atcgacgagc tcaaggccat tgcgtgcgga
     3781 attgtactgc gctttgattc cgagtccggc ggcgttgatc gcatcgatca catcgtcgag
     3841 cgtttccgcg gcggcgaggt tgaccgtcgc cgaggcgccg ctgcggtcgg tcaggctgag
     3901 ctgaccgagc gtccccaggc cattgccgcc ccccagcgtg ccgagcagcg tggtctttag
     3961 cccggaaatc agccgccggc cgctgatgct atcgccgctt gcggggccga cgaggcccag
     4021 ctcagccgcg gtgctgccac ccaccgaatt gctgacggcg aacgtgccgc cgttgtccgt
     4081 cgtcaggtcc gtcagttcga tccgcttgcc gtcggc
//
LOCUS       DASUGB010000682         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_631591, whole genome shotgun sequence.
ACCESSION   DASUGB010000682 DASUGB010000000
VERSION     DASUGB010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631591"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV87_13020"
     CDS             <1..189
                     /locus_tag="VFV87_13020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5104733.1"
                     /translation="GIVEAMDQRGQQFGASRLKSLIHEQHNAPLPELVRNIGRQVELH
                     YVGDSPPDDLTVVALRRQ"
     gene            236..730
                     /locus_tag="VFV87_13025"
     CDS             236..730
                     /locus_tag="VFV87_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5104734.1"
                     /translation="MSTLQHAITNWQFNRPRTLGLLEKIEQQPDPQKVLSWRPGPGRA
                     HLAWQFMHIGITEELFATERLVPGTQPAFADLVPRFRGGSTPDDNIPPASLIRQILTD
                     SREHLLATISRFSDGDLGSTFQIAKDRQLSLSDVLHILAWHEPHHQGQAHITFNLYKS
                     MKPA"
     gene            complement(748..2427)
                     /gene="ilvD"
                     /locus_tag="VFV87_13030"
     CDS             complement(748..2427)
                     /gene="ilvD"
                     /locus_tag="VFV87_13030"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5104735.1"
                     /translation="MAQLNKVSSHITQPKSQGASQAMLYATGLKPEDMHKAQVGIASV
                     WYEGNPCNMHLLKLGEKVKEGVTAAGLVGMRFNTIGVSDGISMGTEGMCYSLQSRDLI
                     ADSIETIMGAQWYDGLIALPGCDKNMPGCLMAMGRLNRPAIMVYGGTIKAGCTPRHPK
                     LDIVSAFQVYGEFIAGKISDEEREEIVRCSIPGAGACGGMYTANTMASAIEALGMSLP
                     YSSSIPAEDPLKQGECLAAGKAILNLLEKDLKPRDIMTRAAFENAMVIVMALGGSTNA
                     VLHLIAMARAVGVPLTIDDFQKVSDRVPYIADLKPSGKYVMEDLHHVGGTPAVMKYLL
                     EKGLLDGSCLTVTGKTIAQNLVEAKGLADGQRVIHPLEQPIKKTGHIRIMRGNFCPDG
                     AVAKITGKEGLKFTGTARCYDSEEDMLHGLERKEIQKGDVIIIRYEGPKGGPGMPEML
                     TPTSAIMGAGLGEHVALITDGRFSGGSHGFLVGHVTPEAQDGGPIALVKNGDQITIDA
                     EANRIDLAVSADDLAQRRAAFQAPPYKFTRGTLYKYIKNVKTATEGCVTDE"
     gene            2468..>2722
                     /locus_tag="VFV87_13035"
     CDS             2468..>2722
                     /locus_tag="VFV87_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104736.1"
                     /translation="MRQQNLNLVQYRGGGYDALSQAEWRATDLVVDRTNFFSHMRMSV
                     KLVSIITYGECSADAVLHQCIACMNRWPLPLADGVISRQHQ"
BASE COUNT          467 a          901 c          806 g          548 t
ORIGIN      
        1 ggcatcgtcg aggcgatgga ccagcgcggc cagcagttcg gcgccagccg gctgaagtcg
       61 ctgatccacg agcagcacaa cgccccgctg ccagaactgg tccgcaatat cggccgccag
      121 gtcgagctgc actacgtggg cgacagcccg ccggacgatc tcaccgtcgt cgccctgcga
      181 cggcagtagg tccttcgcac tttccgttgt ttcactcaat ccaatccgaa atcccatgag
      241 cactctccag cacgcgatca ccaactggca gttcaatcgc ccccgcacgc ttggcttgct
      301 cgaaaaaatc gaacaacagc cggacccgca gaaagtcctc agctggcggc cggggccggg
      361 ccgggctcat ctcgcctggc aattcatgca catcggcatc accgaagagc tcttcgccac
      421 cgagcgtctt gtgcccggca cccagcccgc ctttgccgac ctggtgccgc ggttccgcgg
      481 cggcagcacg cccgatgaca acattccgcc cgcgtcgctg atccgccaga tcctcaccga
      541 ttcgcgcgag cacctgctcg ccaccatcag ccgcttcagc gatggcgacc tgggcagcac
      601 gttccagatc gccaaagacc gtcagctctc gctgtccgat gtcctacaca tcctcgcctg
      661 gcacgagccc caccatcagg gacaagcaca tattacgttc aatctctaca aatcgatgaa
      721 acctgcatga cggcttcgcc aagctaatca ctcatccgtc acgcacccct ccgtggcagt
      781 cttcacgttc ttgatgtact tgtaaagcgt gccgcgggtg aatttgtaag gcggggcttg
      841 gaaggcggcg cgacgctggg cgagatcgtc ggccgatacg gcgaggtcga tgcggttggc
      901 ttcggcgtcg atggtgatct ggtcgccgtt tttgaccagg gcgatggggc cgccgtcctg
      961 ggcttcgggc gtgacgtggc cgacaagaaa gccgtggctg ccgccgctga agcggccgtc
     1021 ggtgatcaag gcgacgtgct cgcccaggcc ggcccccatg atggcgctgg tcggcgtgag
     1081 catctccggc atgcctgggc cgcccttggg gccttcgtag cgaatgatga ttacgtcccc
     1141 cttctgaatc tccttccgct ccaagccgtg gagcatgtct tcttcgctgt cgtagcagcg
     1201 agccgtgccg gtaaacttca gcccctcctt gcccgtgatt ttcgccaccg cgccgtccgg
     1261 gcagaagttg ccccgcatga tgcggatgtg ccccgtcttc ttgatcggct gctcgagcgg
     1321 atggatgacg cgctgcccgt ccgcgaggcc tttcgcctcg accaggttct gcgcgatcgt
     1381 cttaccagtg accgtcaggc agcttccatc gagaagaccc ttctcgagca ggtatttcat
     1441 caccgccggc gtgccgccga cgtggtgcag gtcctccatc acgtatttgc cgctcggctt
     1501 gaggtcggcg atgtacggga cgcggtcgct caccttctgg aaatcgtcga tcgtcagcgg
     1561 cacgccgacg gcgcgggcca tcgcgatcag gtgcagcacg gcgttcgtcg agccgccgag
     1621 ggccatcaca atcaccatcg cgttctcgaa cgcggcccgc gtcatgatgt cccgcggctt
     1681 gaggtctttc tccaggagat tcaggatcgc cttgcccgcc gccaggcact cgccttgctt
     1741 gagcgggtct tcggcgggaa tcgacgaact gtacggcagc gacatgccca gggcctcgat
     1801 cgcgctggcc attgtgttgg ccgtgtacat cccgccgcag gcccctgcgc cgggaatcga
     1861 gcagcggacg atttcctcgc gctcctcgtc gctgattttg cccgcgatga attctccata
     1921 gacctggaac gcgctgacga tgtcgagctt cggatgccgc ggcgtgcagc cggccttgat
     1981 cgtgccgcca tagaccatga ttgccgggcg gttcagccgc cccatcgcca tcaggcagcc
     2041 gggcatgttt ttgtcgcagc cgggcagggc gatcaggccg tcgtaccact gggcgcccat
     2101 gatcgtctcg atcgaatcgg cgatcaggtc gcgggactgc agcgagtagc acatcccctc
     2161 cgtccccatg ctgatgccgt cgctgacgcc gatggtattg aaccgcatgc cgaccagacc
     2221 cgccgccgtc accccttcct tcaccttctc gcccagcttg aggaggtgca tgttgcacgg
     2281 gttcccctcg taccacacgc tggcgatccc cacctgggcc ttgtgcatgt cctccggttt
     2341 cagaccggtg gcatagagca tggcctgcga cgccccctgg ctcttgggtt gcgtgatgtg
     2401 gctggacact ttgttgagct gggccatggc ggggtcgtaa ggtgggagaa agaacggatt
     2461 caccgtcgtg agacagcaaa acctgaattt agttcaatac cgcggtggcg gctacgacgc
     2521 actgagtcaa gctgaatggc gggcgactga cttagtcgtg gatcgcacaa attttttctc
     2581 ccacatgcga atgtccgtaa aacttgtatc cataatcact tatggcgagt gctcggcgga
     2641 tgccgtgctg catcaatgca tcgcgtgcat gaatcgttgg ccgctgccat tggcggatgg
     2701 agtcatttca cgacaacatc ag
//
LOCUS       DASUGB010000683         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_910335, whole genome shotgun sequence.
ACCESSION   DASUGB010000683 DASUGB010000000
VERSION     DASUGB010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910335"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..986
                     /locus_tag="VFV87_13040"
     CDS             228..986
                     /locus_tag="VFV87_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104737.1"
                     /translation="MSLIELRNICKTYDLGEVQVEALKDTDLTIEQGEYVALVGPSGS
                     GKSTLMNTLGCLDRPTRGSYKLAGVEIATMSRDERARLRNQRLGFVFQNFNLLARTTA
                     LENVELPLLYARGLSARERRQRATEKLELVGLAERMGHHPSQLSGGQQQRVAIARALV
                     NEPSILLADEPTGNLDTRTSQEIIALFRQLNEEQGITIILVTHEQDVAASAKRTVVLR
                     DGRIVADTLDFAQALQALHSSPQTETAAEVESTD"
     gene            1031..2971
                     /locus_tag="VFV87_13045"
     CDS             1031..2971
                     /locus_tag="VFV87_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5104738.1"
                     /translation="MRARTTRGAITAAIFALALSLAPLASAQPGIGTGPSQTRLPYRV
                     IYPGQRTVQYRDPSQFPFIPLQPSSPPPTVSRPPTGDVRYLSLDDAIRICLENDDVIR
                     VLTGVTAVASGRTIYDPAIVNTTIDEQQARFDPFLNVSQAWDRFEPPSAVFVDPNNPA
                     AGTIITGVRTDDYDLNVGLSKQNPLGGQWDFGLSVVERRFQPGIFPLNPQTTSATSLA
                     YTQPFLKGAGMRANLAPVVLARIDTERSFFQLKSAVQDQVQSLIDGYWNLVAARVDVW
                     AIEQQIEQAEFAYRLAAAEQEVGRKNASDKAQALSSLSTFRANLISAKNLVLQREAAL
                     RNLLGLPPWDEATLVPSTEPVFDHFEPDWLALVDLAAQRRPDLIELKLILEADEQQLI
                     IANNQALPQLDAVGLYRWNGLSGELPTGNGISSGPGQFTDWTLGVNFSVPLGLRQARA
                     VLRQRELVLARDRANLDQGLHAASHDLATTIRSLDQAYEQFLAFRAAREAARENLRIQ
                     EGIFRSGFRESFNFLNVLVAMSDWGNSVRAEAAAVAEYNSLLAELERQTGTILETHGV
                     RFYEERYGSIGPLGRLARPVCYPESTPPTLNYDRYPPGDRPAEQSFNLKVPVMLPGRQ
                     QPPPAGEEIPPPAPQPRLPRER"
     gene            2982..3674
                     /locus_tag="VFV87_13050"
     CDS             2982..3674
                     /locus_tag="VFV87_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5104739.1"
                     /translation="MCGRYTLRTPMTVLAQQFLFELDASLEGSGPRYNIAPTQDVAAV
                     RIREAGGKRELAFFHWGLIPPWAKDAKIAYSTINARADTVAAKPAFRAAFKKRRCLVL
                     ADGFYEWQRSGKTKLPFLYEVDGGKPFALAGLWESWRGPEKGDGPPLESCALITTDAN
                     ELASQIHDRMPVILDAADYDAWLDPENQDRDGLEALLAPFPAERMTARAVSTVVNNAR
                     NDGSECVELRST"
     gene            3816..>4255
                     /locus_tag="VFV87_13055"
     CDS             3816..>4255
                     /locus_tag="VFV87_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5104740.1"
                     /translation="MRLLAIVAMVLGAAAPCLAQGIQIWPSEVKLAGPFARQQVLISQ
                     EGNDVTHEATFASSDPSIVAIRDHGYLVPLGDGEATITVMHDGMSSAAKVTVSGFAQP
                     QTVDFQGEIVPLLSRYGCNSGGCHGKASGQNGFKLSLFGFDVAW"
BASE COUNT          795 a         1400 c         1346 g          714 t
ORIGIN      
        1 cctgctctac gaaatccaga aggcccagac cgacgtccac gagcagacca acaaagagcg
       61 ccaggagcgg atcaagcgga tcctcgaagg cgacgccgct aagccgagcg agacgcccaa
      121 acccggcgat acgccgcagc cgtagcggca actgaccggc ttggcatcga ctagcggatg
      181 agtaatctga ggaaaagaat cctcaagctg cgcatcggac gtttcccttg tccctcatcg
      241 agctccgcaa catctgcaag acgtacgacc tgggcgaggt gcaggtcgag gcgctgaagg
      301 ataccgacct gacgatcgag cagggtgagt atgtcgccct ggtcgggccg tcgggctccg
      361 gaaaatcgac gctgatgaac acgctcggct gcctggaccg gccgacgcgg gggagctaca
      421 agctggcggg cgtggaaatc gccaccatgt cgcgtgacga gcgggcccgc ctgcgtaatc
      481 agcggctggg gttcgtgttt cagaacttca acttgctggc ccggacgacg gcgctggaga
      541 acgtcgaatt accgctgctg tatgcccgcg gcctctcggc ccgcgagcgg cggcagcggg
      601 cgacggaaaa gctcgagctc gtcggcctgg cggagcggat gggccaccat cccagccagc
      661 tttccggcgg ccagcagcag cgggttgcca tcgctcgggc cctggtcaac gagccgtcga
      721 tcctgctggc cgacgagccc acgggcaacc tcgatacgcg cacgagccag gagatcatcg
      781 ccctctttcg ccagctcaac gaggagcagg gaattacgat catcctcgtc acccacgagc
      841 aggacgtggc cgccagcgcc aaacggacgg tggtgctgcg cgacgggcgg atcgtggccg
      901 acacgctcga ctttgcccag gcgttacagg cgctacattc gtcaccgcaa accgaaacgg
      961 cggctgaggt tgaatcgaca gattgagatt ccgtggcagg cccgccaaat caggccgaat
     1021 cgatctaggt atgcgtgcgc gcacaactcg cggtgctatc acggcggcaa tctttgcgct
     1081 ggcgctcagc cttgcgccac tcgcctctgc gcagcccgga atcggcacgg gtccaagcca
     1141 aacgcgcctg ccttaccgcg tcatttatcc tgggcaacgc accgttcagt accgtgaccc
     1201 aagccagttc ccgttcattc cgctgcagcc cagctcgccg ccgccgaccg tcagccgccc
     1261 gccgaccggg gatgtgcgat atctctcgct ggacgacgcg atccgcatct gcctggagaa
     1321 tgacgacgtg atccgggtgc tgacgggcgt caccgccgtc gccagcggcc gcacgattta
     1381 cgacccggcg atcgtcaaca ccaccattga cgagcagcag gcccgtttcg atccgttcct
     1441 caacgtgagc caggcgtggg accgcttcga gccgccgtcg gcggtgttcg tcgatcccaa
     1501 caacccggct gcggggacga tcatcacggg cgttcgcacg gacgattacg acctgaacgt
     1561 cggcctatcc aagcagaacc cgctgggcgg gcaatgggat ttcggcctga gcgtggtcga
     1621 gcggcgattt cagccgggca tctttccgct gaacccgcaa accacttcag cgacaagtct
     1681 ggcctacacg cagccattcc tcaagggagc cggaatgcgg gccaacctgg cgccggtcgt
     1741 gctcgcgcgg atcgacaccg agcggtcgtt ctttcagctc aaatcggccg tgcaggacca
     1801 ggtgcagagc ctgattgacg gctactggaa tctcgtggcg gcgcgggtcg atgtgtgggc
     1861 catcgagcag caaatcgagc aagccgaatt cgcctatcgc ttggctgcag ccgagcagga
     1921 ggtgggccgc aaaaacgcct ctgacaaggc ccaggcgctc tcttcgcttt cgaccttccg
     1981 ggccaatctc atttccgcga agaatctcgt cctgcagcgc gaggcggcgt tgcgaaatct
     2041 cctcggcctg ccgccatggg acgaagccac actggtccct tcgaccgaac cggtctttga
     2101 ccacttcgag ccggattggc tggcgctggt cgatctggcg gcccagcgaa gaccagacct
     2161 gatcgagctg aagctcattc tggaagccga cgagcagcag ctcattatcg ccaacaacca
     2221 ggcgctgccg caactcgacg cggtggggct ctatcgctgg aatggactgt ccggcgagtt
     2281 gcccaccggc aacggcatct cgagcgggcc ggggcaattt accgactgga cgctgggcgt
     2341 gaacttctcg gtgccgctcg gcttgcggca ggcccgcgcg gtgctgcggc agcgggagct
     2401 tgtcctggcc cgcgaccggg cgaatctcga ccaggggctg cacgccgcga gtcacgacct
     2461 ggcgacgacg atccgcagcc tggaccaggc gtacgagcag ttcctggcgt tccgcgccgc
     2521 ccgcgaagcg gcgcgcgaga acctgcggat tcaagagggt atcttccgca gcggattccg
     2581 cgaatcgttc aactttctca acgtgctggt ggcgatgtcc gactggggca actcagtccg
     2641 cgccgaagcg gcggctgtgg cggagtacaa ctcgctcctg gcggaactgg agcgccagac
     2701 ggggaccatc ctggaaacgc acggcgttcg gttttacgag gagcggtatg ggtcgatcgg
     2761 cccactggga aggctggcgc ggcccgtctg ttatccggag tcgacgccgc ccacgctcaa
     2821 ctacgaccgc taccctcccg gcgaccgccc tgccgagcag tccttcaacc tcaaagtgcc
     2881 ggtcatgctc cccggccgcc agcagccgcc tcccgccggc gaagagattc cgcccccggc
     2941 gccgcaaccc cgcttgccgc gagagcggta gaatcgaacc aatgtgcggc cgatacacgc
     3001 tgcgaacgcc gatgacggtg ctcgcccagc agttcctgtt cgagctcgac gcttctctcg
     3061 aaggctccgg cccgcgctat aacatcgccc ccacgcaaga tgtggcggct gtccgcattc
     3121 gcgaagccgg cggaaagcgg gagttggcct ttttccactg gggactgatc cccccgtggg
     3181 ccaaggacgc caaaatcgcc tacagcacca ttaacgcacg cgccgatacc gtcgccgcca
     3241 agccggcctt ccgcgcggca ttcaagaagc gccgctgcct ggtgctggcc gacggcttct
     3301 acgaatggca gcgcagtggc aaaacgaagc tgccgttcct ctacgaagtg gatggcggga
     3361 agcccttcgc gctggccggc ctgtgggaat cgtggcgggg gccggagaag ggggacggcc
     3421 cgccgctgga gtcgtgcgcg cttataacga ccgacgcgaa cgaacttgcc agccagattc
     3481 acgaccggat gccggtgatt ctcgacgcag ccgattatga cgcctggctg gatcctgaga
     3541 atcaagaccg cgacgggctc gaggcactac tcgcaccctt cccggcggag cgaatgacgg
     3601 cccgtgccgt gagcacggtc gtcaataatg cccggaatga tggatcggag tgtgtcgagc
     3661 tacggtccac ctagcgcgcc gaccgggccg agtgcctatc acgtcacatt cgccgctgtt
     3721 gcgattggca gcggccggca ctacgataga tcaagggtcg tcgatccttc ccacctttgc
     3781 ggcgcctcga tcccacctca aatcacgcaa tgcgaatgcg cttgctggct atcgtggcaa
     3841 tggtgctggg cgcagcagcg ccgtgcctgg cccaaggcat acaaatctgg ccgagcgaag
     3901 tgaagctcgc tggcccattt gcccgccagc aagtcttaat ttctcaggag ggcaacgacg
     3961 ttacacacga ggcgacgttc gcctcttcgg acccgtcgat cgttgcgatt cgcgatcacg
     4021 gttatctggt gccgctgggc gatggcgagg cgacgatcac cgtgatgcac gacggcatgt
     4081 cctctgcggc aaaagtgacc gtcagcgggt ttgcccagcc gcagacggtc gattttcaag
     4141 gcgaaatcgt tcccctcctc agtcgctatg gctgcaattc cggcggctgc catggcaagg
     4201 cgagcgggca gaacggcttc aagctctcgc tgtttggctt cgacgtggcc tggga
//
LOCUS       DASUGB010000684         5889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2022304, whole genome shotgun sequence.
ACCESSION   DASUGB010000684 DASUGB010000000
VERSION     DASUGB010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022304"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFV87_13060"
     CDS             complement(<1..608)
                     /locus_tag="VFV87_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX5104741.1"
                     /translation="MPGFEILHEDAAVLVVNKPAGMLTQAPLGIDSLEVRIKRLLHER
                     EASDDEIYLGVPHRLDRPVSGVIVFAKTLKAAQKISRQFEKRWVEKIYWALVEGSVEP
                     QAGTWTDHLKKVYGKPRSEVVEVSDPLGQIAILHYQTIQQFADKTLLQIQLETGRTHQ
                     IRIQCACRGHPLLGDELYGSGVPFGPWSDDERERLIALHARSL"
     gene            complement(650..2122)
                     /locus_tag="VFV87_13065"
     CDS             complement(650..2122)
                     /locus_tag="VFV87_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104742.1"
                     /translation="MSRRRNRREFLEDSMFAAAAAAAAGSSGRLLAGESDVQSKSPSE
                     RLHVAVVGVKGRGGSHIGAFAGRQDTLITHVVDIDSNIGPQRAKEVGKRQGGIEPQWV
                     EDVRKVLEDKSVDIVTIATPNHWHSLMAIWAMQAGKDVYVEKPVSHNVSEGRRAVEAA
                     RKYKKICQTGTQSRSNPGMIQAIEFVRSGKIGDVKVARGLCYKRRDSIGPKGTYEIPK
                     NINYDLWSGPAPILPLTRKNLHYDWHWIWAYGNGDLGNQGIHQMDIARWGLGVNTLSK
                     AAFSYGGRLGYEDAGETANTQVVLHDYDKQSLVFEVRGLVTDDYRGAKVGVIFEGTDG
                     YVVLPSYNGGAAFDKDGGKIAEFSGGGDENHFANFLKAVRSRKLADLNADIEEGHLSS
                     ALCHTGNISYQLGSLMSADELDKAVASLQTTDNAKDTLERTLAHLKDNKVDMAATKLQ
                     VGPVLQCDPQTESFPGNAAANALLTREYRKPFVVPPAGLV"
     gene            complement(2804..3022)
                     /locus_tag="VFV87_13070"
     CDS             complement(2804..3022)
                     /locus_tag="VFV87_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104743.1"
                     /translation="MAHVLARLDQTGDDMTSGPTITIVRLRREIERKDQTPVDPEQEV
                     KKSTAIRNLRYMIELLADPAFMSKAVGP"
     gene            complement(3418..4347)
                     /locus_tag="VFV87_13075"
     CDS             complement(3418..4347)
                     /locus_tag="VFV87_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104744.1"
                     /translation="MGLSKQKPARRIPQAVQAVRILLRPQNRGPVLAATVLAAALAGA
                     LYAWQRWGEPGLSSAEYLVTPDKILATPQPVWIRADVKADVVRTANLAERNLRDPRLV
                     EEVAAAFALHPWVAAVSRVEKRYPARIEVALEYRRPVAVVEISGAGEAGLLFIDAGGV
                     LLPSEDFAPGQTKDYLRIAAGGETPAGIYGTASWSERILGAARIAAAWEGAEDRGQGT
                     GDRGQGTGDSGSKLWQKLGLYRIVPVQSSAGGVTYELRTERDARVLWGSAPGSEAPGE
                     PPPEQKIAKLQHLVADKGPLDREGASSPVDLRR"
     gene            complement(4420..5298)
                     /gene="murB"
                     /locus_tag="VFV87_13080"
     CDS             complement(4420..5298)
                     /gene="murB"
                     /locus_tag="VFV87_13080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5104745.1"
                     /translation="MPIPAGFEHITRENEPLAPLTWFRLGGAAQYFAEPTSVEELAAL
                     VKACHQQGLPIRILGGGSNLLVREEGVSGAVVSLSAATFGRISVSGRRITAGGGAKLG
                     HVISTAVREGLAGLEPLVGIPGTLGGALHSNAGTMGGDIGQHTTSATVLTRKGEIVTR
                     SGAELRFGYLRSSLDELSILEATLDLEPADSALLTKQMQQAWILKRSAQPRSDQNTGL
                     VFKSPGGTSAAQLIEDAGLGSARVGEAEISDRNANFIVVGPKAKASDVLELIEVVRKG
                     VADKSGVELELALEVW"
     gene            5460..>5889
                     /locus_tag="VFV87_13085"
     CDS             5460..>5889
                     /locus_tag="VFV87_13085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5104746.1"
                     /translation="MHFARLILLGLALVTLAVAAWLTGCGDSPKTPVAEGSSTSESAP
                     ESVSTAGVAPKQTKPSTPPKPVDPIVILHTSAGQIKVQLFAEKAPQTVDNFLNNYARR
                     GFYDETIFHHIEPGMMLIAGGFTADLQPKETRAAIYNESRN"
BASE COUNT          985 a         1963 c         1787 g         1154 t
ORIGIN      
        1 aggctccggg cgtgcagggc aatcagccgc tcgcgctcgt cgtcgctcca cgggccgaac
       61 ggcacgccgc tgccgtagag ttcgtctccc aggagcggat ggccgcggca ggcgcactga
      121 atgcgaatct ggtgcgtgcg accggtttcg agctggatct ggaggagcgt tttatcagcg
      181 aattgttgaa tggtctggta gtgcaggatc gcgatctggc cgagcggatc gctcacctcg
      241 acgacttcgg aacgcggctt gccatagacc ttcttcaagt gatcggtcca cgtgccggct
      301 tgcggttcga cggagccttc gaccagcgcc caatagattt tctccaccca ccgtttctca
      361 aactgccgac tgatcttctg ggcggctttg agcgtcttcg caaagacgat cacgccggaa
      421 accggccggt cgagccggtg cggcacgcca agatagattt cgtcgtctga tgcttcccgc
      481 tcgtgcagca gccgcttgat gcggacttcc aggctgtcga ttcccagcgg ggcctgtgtg
      541 agcatccccg ccggcttgtt gacaaccagc accgccgcat cttcatgcag aatctcgaag
      601 ccgggcatgg caacgtgggg caggcatcct tgcctgccgg cgagattccc tacaccagcc
      661 ccgccggcgg cacgacgaac ggcttgcgat actcgcgagt aagcagggcg ttcgccgcgg
      721 cgttgccagg gaagctctcg gtctgcggat cgcactgcag gaccgggccg acctgcagct
      781 tggtggcggc catatcgacc ttgttgtcct tgaggtgcgc cagcgtccgc tcgagcgtgt
      841 ccttggcgtt gtcggtggtc tgcaacgagg ccaccgcctt atccagctcg tcagccgaca
      901 tcagcgagcc gagctggtac gaaatgttgc ccgtgtggca cagggcgctc gataggtgcc
      961 cttcctcgat gtcggcgttc aggtcggcca gcttgcggct gcggacggct ttcaggaagt
     1021 tggcgaagtg gttctcgtcg ccaccgccgc tgaactcggc gattttgccg ccgtccttgt
     1081 cgaacgccgc accgccgttg tagctgggca gcacgacgta gccgtcggtc ccttcaaaga
     1141 tcacgccgac tttcgctccg cgataatcgt ccgtcacgag gccgcgaacc tcgaacacca
     1201 gcgactgctt gtcatagtcg tgaagtacga cctgcgtgtt ggccgtctcg cccgcgtctt
     1261 cataacccag gcggccgccg tagctgaatg cggctttgct gagcgtgttc acgcccaggc
     1321 cccagcgagc gatgtccatc tggtgaatgc cctggttccc caggtcgccg ttgccgtagg
     1381 cccagatcca gtgccaatcg taatgcagat tcttccgcgt cagcggcagg atgggggccg
     1441 ggcccgacca gaggtcgtaa ttgatgttct tgggaatctc gtacgtcccc ttggggccga
     1501 tcgaatcgcg ccgcttgtag cacaagccgc gggccacctt cacgtcgccg atcttccccg
     1561 agcggacgaa ctcgatcgct tggatcatgc cgggattgct gcggctttgc gtgccggtct
     1621 ggcagatctt cttgtacttg cgggccgctt cgaccgcccg gcggccctcg ctcacgttgt
     1681 ggctcaccgg cttttcgaca tagacgtcct tgccggcctg catggcccaa atcgccatta
     1741 gcgaatgcca gtggttgggc gtggcgatgg tgacgatatc gaccgacttg tcctcgagga
     1801 ccttccgcac gtcctctacc cactggggct caataccgcc ttgccgcttg ccgacttcct
     1861 tggcccgctg cggcccgata ttgctgtcga tatcgacgac gtgcgtgatc agcgtgtcct
     1921 gccggccggc gaaggcgccg atgtggctgc cgccgcggcc tttgaccccc accaccgcca
     1981 cgtgcaggcg ctcgctgggg cttttgctct gcacgtcgct ttcgccggcc agcagcctgc
     2041 cggaactccc cgccgccgcc gctgccgcgg ccgcaaacat cgaatcttcc aggaattcac
     2101 gacgatttct tcggcgggac atggcattcg ctccaacgta tcaggaaggc gggaaaggca
     2161 ccaaactggc gggccgcacg gacagcgggt aatcgggcat tttacccaga ggaccagcat
     2221 ccttgccagc atccacggcc cagcgtagcc ggtagccaat actggctgag ttgaaagtaa
     2281 cccaacgcgt aagcgacggt cgaccgtaag cgcaactttc cgaactattt cgcaacccaa
     2341 cccccctgcg caacattttc gcaactcgct tcccgacaac acgttgcgcc gcccaacgcc
     2401 ccccgtccga ggatgttgcg ctgttgcgcg tcgtcaatca tttttcctcc attttctcac
     2461 cctctccgct gggagagtgc cagagtgagg gtgctgtggg caatgggccg actctcccct
     2521 cgtccgttgt aggctccaac tgcctcctga cccttcgcac gcacctcctc ccattcctat
     2581 agatgcgcac ggacgttcat ttattcccga aatctgcaaa aactttcgac ccatattgaa
     2641 gcctggccct ttgcggcgac ggtgagactg atcgcggcca gactccgctg tgttttggca
     2701 gccaaaacac agccacccaa caaacgtgtg cacgcgatga ttgtcaaacg cccgtaggcg
     2761 ctgcccgaag gtcgcgtatc gggcaaatct ccgtttctac gtgctacggg ccaaccgctt
     2821 tggacatgaa agcggggtca gcgagcaatt caatcatgta ccggagattt cggattgccg
     2881 tcgatttttt cacttcctgc tcgggatcga cgggggtctg gtccttgcgt tcaatctccc
     2941 ttcggagccg tacgatcgta atcgtcggcc cggaggtcat gtcgtcgccg gtctgatcaa
     3001 ggcgggccaa gacatgagcc atgaggtgca attggcgatc tgatgccccg actaccgcac
     3061 tcaaaatctt gcgccttgcc gggactccca cgttgatcaa ggcgttcgcc gcggtatacg
     3121 gcgaaagcgg gtcccgatgc gacaaagggg ataagtccaa ttatttgccc tgccgcgggg
     3181 agggcgacaa aggggataag tccaattatt tgccctgccg cgggcggccg cggggggcgg
     3241 aaggtggatt ctaatcccag ccgccgggcg acgcgattct gccacgcggg acctgccgta
     3301 gggccggccg tgctgcacgc tggtccgctg tgcctcggtc tgcggctggt tgactgaaat
     3361 cgcctgtcca acgcaatctt gagaaattcg gcacctcgct ggcagccgta ctgcggccta
     3421 ccggcgaaga tccaccggcg aagatgctcc ctcgcggtcg agcgggcctt tgtcggctac
     3481 gaggtgctgg agtttggcga tcttttgctc gggcgggggt tcgcccggcg cttcgctgcc
     3541 gggggcggag ccccaaagga cgcgggcgtc acgctcggtg cgcagctcgt acgtgactcc
     3601 gcccgcggat gactggaccg gcacgatccg atacaagccc agcttctgcc acaacttgct
     3661 cccgctgtct cctgtcccct gtcccctgtc ccctgttccc tgtcctctgt cctctgcccc
     3721 ttcccacgcc gcggcgatcc gcgccgcccc aagaatccgc tcgctccacg aggccgttcc
     3781 gtaaatgccc gctggcgtct ctccgccggc ggcgatacgc agatagtcct tcgtctgtcc
     3841 cggggcgaaa tcttccgagg ggagcaagac gccgcccgcg tcgatgaaga gcaggccggc
     3901 ttcgcccgcg ccggagattt cgaccacggc aaccgggcgg cgatattcga gcgcgacctc
     3961 gatgcgggcc ggatagcgtt tttcgacacg gctgacggcg gcgacccacg ggtgcagggc
     4021 aaatgccgcg gccacctcct cgaccaagcg cgggtcacgg agattgcgct ccgcgaggtt
     4081 cgccgtccgc acgacgtcgg ctttgacgtc ggcgcgaatc cagacgggct ggggcgtggc
     4141 gagaatcttg tcgggcgtca cgaggtactc ggcggagctc agccccggct cgccccaccg
     4201 ttgccaggcg tagagcgctc ccgccagagc cgcggcgagc actgttgccg ccagaacggg
     4261 gccgcgattc tgcggccgca agaggatgcg tacggcttgc acggcctgcg gaatcctccg
     4321 cgccggcttc tgcttcgata atcccatggc ccgaactcct tttcgggata ttttggagta
     4381 ctgcgactgg tcgcagccag tcgccgcact ccaaagtctt caccaaactt ccagcgccag
     4441 ttccaactcc acgccgctct tgtcggcaac ccccttgcgc acgacctcga tcagctccag
     4501 cacgtcgctc gccttggctt tggggccgac gacgatgaaa ttcgcgttgc ggtcgctgat
     4561 ttccgcttcg ccgacgcggg ctgatcccag gccggcatct tcgatgagct gcgcggcact
     4621 ggtgccgccg ggactcttga agactagccc agtgttctgg tcgcttcgcg gctgagcgga
     4681 cctcttcagg atccacgctt gctgcatctg cttcgtcaac agggccgaat cggccggctc
     4741 caggtcgagc gtcgcctcca ggatggagag ctcgtcgagg ctgctgcgga ggtagccgaa
     4801 ccgcagctcg gccccggagc gcgtgacgat ttcgcctttg cgggtcagga ccgtcgcgct
     4861 cgtcgtgtgc tggccgatat cgccccccat cgtgccggca ttgctgtgca gcgcaccgcc
     4921 cagcgtgccg ggaatcccca cgagcggttc caggcccgcc aggccttcgc ggaccgccgt
     4981 ggaaatgacg tggccaagct tggcgccgcc gccggcggtg atccggcgac cgctgacgct
     5041 gatccgcccg aacgtcgccg ccgagagcga taccaccgcc ccgctcaccc cttcctcgcg
     5101 gaccagcagg ttcgacccgc cgcccagtat gcggatcggc aagccctgct gatggcacgc
     5161 tttcaccagg gccgccagtt cctccaccga ggtcggttcc gcgaaatact gcgccgcccc
     5221 gcccagccga aaccacgtca gcggggccag cggctcattt tcgcgggtaa tgtgctcgaa
     5281 gccggcggga atgggcatgg cggaccatcg acaactggga atctggagga accttgtcgg
     5341 attctacagc cgcggtccgg acgcgtcagc atcgcaaatt gctgttgcac gaaacgtcca
     5401 gccggccgat gatgccctcc ccgtaagacc cgcgcgccgc aagcagggag gcttttgcga
     5461 tgcattttgc ccggctgata ttgctcggtt tggcccttgt cacgctcgcc gtcgccgcct
     5521 ggctgacggg ctgtggcgac tcgcccaaaa cgccggtagc agagggcagt tcgacttctg
     5581 aatccgcgcc ggaaagtgtt tccaccgcag gagttgcgcc gaaacaaacc aagccgtcca
     5641 cgccgcccaa gccggtcgat ccgatcgtca tcctgcacac ctctgccggg cagattaagg
     5701 tccagctctt cgccgaaaag gcgccccaaa cggtcgataa tttcctgaac aactatgccc
     5761 gtcgtggctt ttacgacgag acgatttttc accacattga gccgggaatg atgctgatcg
     5821 cgggcgggtt tacggccgat ttgcagccga aggagacgcg ggcggcgatt tacaacgaat
     5881 cacggaacg
//
LOCUS       DASUGB010000685         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_494313, whole genome shotgun sequence.
ACCESSION   DASUGB010000685 DASUGB010000000
VERSION     DASUGB010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494313"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..516)
                     /locus_tag="VFV87_13090"
     CDS             complement(106..516)
                     /locus_tag="VFV87_13090"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5104747.1"
                     /translation="MPDPIPDPRSPNSRPRNWSAKFRDAFRGLWLAVRSERSFSVHLP
                     MAAAVIVLAAVMRVSLVQWCLLGVCITLVLAAETFNTALERLAREIDGESNPEIAAAL
                     DMASGAVLVTAIGAAGIGAAIFLYRLGVLAGWWT"
     gene            580..1428
                     /gene="nagB"
                     /locus_tag="VFV87_13095"
     CDS             580..1428
                     /gene="nagB"
                     /locus_tag="VFV87_13095"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX5104748.1"
                     /translation="MRVIIEPDAEAASRRAARFVAELVRRKPDCVLGLATGSTPLATY
                     RELIRLHKEDGLDFSRVTTFNLDEYVGLGPSHPQSFRYFMQQHLFDALNVDPKRTHVP
                     DGRALDFEAHARQYEQRIKDAGGIDLQILGLGSDGHIAFNEPGSSLGSRTRLKTLASE
                     TIRDNARYFGGEDKVPRLAVTMGVGTILESRRCLLLAFGEHKAAAVRDTVEGPITAQV
                     TASALQLHREVVGIFDELAVSRLARRDYYADVERAQSLLEEGNFGALGIGSAREDKGQ
                     RTEDRS"
     gene            1428..2393
                     /locus_tag="VFV87_13100"
     CDS             1428..2393
                     /locus_tag="VFV87_13100"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5104749.1"
                     /translation="MRQAGVLKDGRDANRFAAWLVSQRIEAHAEQEDGGWAIWVRDED
                     QLPKAREELAKFQASPQDERYRNAQRTAEQVVREEEEQRKRVQGNIVEMRGRWSTGGA
                     VVRRCPLVLGLIGASILVTLFASNDSAPQGAGANTLYDLLLFTDPQAGRSPDGFDVWA
                     SIRRGEVWRLVTPIFIHYGITHLLFNMLVLFTLGGQIENRRGMRFMAFLVLLLAITSN
                     VGQAIELSLTSPGVQFGGMSGVGYGVFGYLLIKVKFDNRDGYMLSQFMTIILIAGFVL
                     SLARSFPEMDSVLSFMPRIANAAHTVGLFVGMALAYAPLLVRRSA"
     gene            complement(2480..2923)
                     /locus_tag="VFV87_13105"
     CDS             complement(2480..2923)
                     /locus_tag="VFV87_13105"
                     /inference="COORDINATES: protein motif:HMM:NF037241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104750.1"
                     /translation="MNKAFVRESDSTDVLCPRCGAIGIEVPRVTVEAHVSAEDRRGLA
                     SAAFFCPTPSCDVAYFDAMEATVAASALLNPVSPKVPAAPLCGCFGLTLADVEADVDE
                     GTPRRIRELLAKSKSPQARCETASPTGRCCLPEVQRAYFKLRGEH"
     gene            complement(3030..3788)
                     /gene="pyrH"
                     /locus_tag="VFV87_13110"
     CDS             complement(3030..3788)
                     /gene="pyrH"
                     /locus_tag="VFV87_13110"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5104751.1"
                     /translation="MATPAESAAVPFQRVVLKLSGESLSHAGERGIGMEEVMHIARPI
                     KQAADAGCQIALVVGGGNILRGSQFKDANAGIQEATAHYMGMLATVINALALQDALES
                     LGCETRVMTAIHMQGVAEPYIRRRARRHLEKGRIVILAAGTGSPFVTTDTAAAQRAME
                     IEADILLKATGVDGVFSDDPKKNPHAVLYSELDYNTVRERNLRVMDSTAIAHCMEHNM
                     PILVFNFRKEGNILRAVRGEKVGTKISSRPAAGN"
     gene            complement(3911..>4176)
                     /locus_tag="VFV87_13115"
     CDS             complement(3911..>4176)
                     /locus_tag="VFV87_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5104752.1"
                     /translation="PLAVCKEEIDPAIVQKEREILKEAALAEGKPASIVDKMVEGRLK
                     NFYAEVALLEQPFVKETSVSVGKHAESKGMKVKKFVHWELGRD"
BASE COUNT          726 a         1294 c         1381 g          775 t
ORIGIN      
        1 catgatcggc cagctcgcag accaagggct gtcggcgaat gcaattgcca gtcatctccg
       61 cctgcccatc ggggaagtgg aatttgccct gggaatgcga accggttaag tccaccaacc
      121 cgccaggacg cccagccggt agagaaagat tgccgcaccg atccctgccg caccgattgc
      181 cgtcaccagg acggctccgc tggccatgtc cagcgcggcg gcgatttcgg ggtttgattc
      241 gccgtcgatc tcgcgggcca ggcgctccag ggcagtgttg aacgtctcgg ctgccaggac
      301 cagcgtgatg cacacgccga gcaggcacca ctggacgagg ctcacgcgca taacagcagc
      361 cagcacgatc actgccgcgg ccatcggcag atggacggaa aagcttcgct cgctgcgcac
      421 ggcaagccac aagccgcgga aagcatcgcg aaacttggca gaccagttac gcgggcgaga
      481 gttcggggat cggggatcgg ggatcggatc gggcatctgt cgcggctggc ggtcagttgc
      541 tattctccag tttgcctgtc aaacgtggga gaatcgcagg tgcgtgtcat tatcgagccg
      601 gacgctgaag ccgccagccg ccgggcggcg cgttttgtgg ccgagctggt ccgccgcaag
      661 cccgactgcg tcctggggct ggcgaccggt agcacgccgc tggcgacgta tcgcgagttg
      721 atccgtctgc acaaggaaga cggcctcgac ttttcgcgcg tgacgacgtt caacctcgat
      781 gagtacgtcg gcctggggcc ctcgcatccg cagagcttcc gctatttcat gcagcagcat
      841 ttgttcgatg cgctcaacgt cgatccgaag cggacgcatg tgcccgatgg gcgggcgctc
      901 gacttcgagg cccacgcccg gcagtacgaa cagcggatca aggacgccgg cggcatcgac
      961 ctgcagattc ttggcctcgg gagcgacggt cacatcgcgt tcaacgagcc cggttcttcg
     1021 ctcggcagcc gcacgcgttt gaaaacactg gccagcgaga cgattcgcga caacgcccgc
     1081 tatttcgggg gcgaggacaa ggttccgcgg ctggcggtga cgatgggtgt gggaacgatc
     1141 ctcgaatcgc gccgctgcct gctcctggcg ttcggcgagc acaaagcggc tgccgtccgc
     1201 gatacggtcg aagggccgat cacggcgcag gtaaccgcct cggcgctgca gctgcaccgc
     1261 gaggtagtcg gcatattcga cgagctggcg gtcagccggc tcgcgcgccg tgactattat
     1321 gcggacgtgg agcgagccca gtcgctgctg gaagagggca atttcggagc gctggggatc
     1381 ggcagcgcgc gagaggacaa aggacagagg acagaggaca gaagctgatg cggcaagcag
     1441 gggtactgaa agacggacgc gatgccaacc gctttgcggc ctggctggtc tcgcagcgaa
     1501 ttgaggccca cgccgagcag gaagacggcg gctgggcgat ctgggtgcgc gacgaggatc
     1561 agttgcccaa ggcccgcgaa gagttagcca aatttcaggc cagtccgcag gacgaacgct
     1621 accgcaacgc ccagcgtact gctgagcaag tcgtccgaga ggaggaagag caacggaagc
     1681 gcgtgcaggg gaatattgtc gaaatgcggg gccgctggag caccggcggg gccgtcgtcc
     1741 gccgctgccc gctggtgctg gggctgattg gggcatccat cctcgtgacg ctcttcgcct
     1801 cgaacgattc ggcgccacaa ggggcggggg ccaatacgct ctatgacctc ctgctattta
     1861 ccgatcccca ggccggtcga tcgcccgatg gcttcgacgt gtgggccagc atcagacgcg
     1921 gcgaggtctg gcggctagtg acgccgatat tcatccatta cggcatcacg cacctcctct
     1981 tcaatatgct cgtgctcttc acgctcggcg gccagattga gaaccgccga gggatgcggt
     2041 tcatggcttt cctcgttctg ctacttgcaa tcacgtcgaa tgtaggtcaa gcaatagaac
     2101 ttagcctgac cagccccggc gtccagttcg gcgggatgtc aggagtcggc tatggcgtct
     2161 tcggctatct gctgatcaag gtcaagttcg acaatcgcga cggctatatg ttgagccagt
     2221 tcatgaccat catcctgatc gcgggattcg tgctgtccct ggcccgcagc tttccggaaa
     2281 tggacagcgt gctcagcttc atgccgcgga tcgccaatgc ggcccacacc gtcgggctgt
     2341 tcgtcgggat ggcgctggcc tatgcgccgc tcctggtgcg gcggtcggca tagcaaacac
     2401 agtaacccga agcgtaagcg aggaagtgag gcgcgttcga ttcatgcctc gcggaacact
     2461 ccctcgctta cgcttcgggt tagtgttccc cgcggagctt gaaataggcc cgctgcactt
     2521 cgggcaggca gcagcggccg gtgggggaag ccgtctcgca ccgggcctgg ggcgatttcg
     2581 atttggcgag cagctcgcga attcgccgcg gcgttccctc atccacgtcc gcttcgacgt
     2641 cggccagcgt cagcccgaaa cagccgcaca agggcgctgc ggggactttg ggactcacag
     2701 ggtttagcag cgcgctggcg gcgaccgtgg cttccatggc gtcgaaatag gcgacatcgc
     2761 agctcggcgt cgggcagaag aatgccgcgc tggccaaccc gcgccggtcc tcggccgaga
     2821 catgggcttc gactgtgacg cggggcactt caatcccgat cgcgccgcac cgcgggcaga
     2881 gcacgtcggt cgaatcggat tcgcggacga aggctttatt catcgccggt cgtaggacag
     2941 gtttccaacc tgtccgaacg agacagttgc cagactggca gtcacggacg ggttggaaac
     3001 ccgtcctacg tttcattgca caaagcccgt tagttccccg cggccggccg gctggagatc
     3061 ttcgtgccga ccttctcgcc gcggacggcc cgaagaatgt tcccctcctt gcggaagttg
     3121 aacaccagga tcggcatgtt gtgctccatg cagtgggcga tggccgtgct gtccatcacg
     3181 cggaggttcc gctcgcggac ggtgttgtaa tcgagctcgc tatagaggac cgcgtgcggg
     3241 ttcttcttcg gatcgtcgct aaacacgcca tcgacgccgg tcgccttcag cagtatgtcc
     3301 gcttcgattt ccattgcccg ctgggcggcg gcggtgtcgg tcgtgacaaa cgggctgccg
     3361 gtgccggcgg cgagaatgac gatgcggcct ttttcgaggt gccggcgagc gcggcgtcgg
     3421 atgtagggct cggctacgcc ttgcatgtgg atcgcggtca tcactcgcgt ctcgcagccg
     3481 agcgattcca gggcgtcctg cagggccagg gcgttgatga ccgtggccag catgcccatg
     3541 tagtgggcgg tggcttcctg gatgcccgcg ttggcatctt tgaactgcga gccgcggagg
     3601 atattgccgc cgccgacgac cagggcgatc tggcagccgg cgtcggcagc ctgcttgatc
     3661 gggcgggcga tgtgcatcac ctcttccatg ccgatgccgc gttcgccggc atggctgagg
     3721 ctttcgcccg agagcttgag gacgacgcgc tggaagggaa cagcggcgga ttcggcaggt
     3781 gtggccatag cgttagtcct cgttcccacg ctccgcgtgg gaacgcccat gctggacgct
     3841 ctgcgtcctc gcggcgcgga gcgccggtgc cttgcggtcc cacgcggagc gtgggaccga
     3901 gatgtcccgt ctagtcccgg cccagttccc agtggacgaa cttcttcacc ttcatcccct
     3961 tgctctccgc atgcttgccg acggaaacgc tggtctcctt gacgaacggc tgctcgagca
     4021 gggcgacttc ggcgtagaaa ttcttcagcc ggccttcgac catcttatcg acgatgctcg
     4081 ccggctttcc ctcggcgagc gctgcttcct tcaagatctc gcgctctttc tgcacgatgg
     4141 ccgggtcgat ttcttccttg cacacggcga gcgggc
//
LOCUS       DASUGB010000686         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1744648, whole genome shotgun sequence.
ACCESSION   DASUGB010000686 DASUGB010000000
VERSION     DASUGB010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744648"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..608)
                     /locus_tag="VFV87_13120"
     CDS             complement(48..608)
                     /locus_tag="VFV87_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104753.1"
                     /translation="MPNVSYPPPIADLITDRLSELGPGSPSEAMRPKLAALSVESLFA
                     NKRVVDQDAARCCLSALWLWHDFLDESHALSQEIDTVDGSYWHGIMHRREPDYGNAKY
                     WFRRVPQHPIHGPLAEQAAQLARANQLDTAAEFLVAQKTWDAFGFVDLCEAIARGKSQ
                     SDQLAREIARLEWRLLFEHCYRQAVV"
     gene            complement(612..3074)
                     /locus_tag="VFV87_13125"
     CDS             complement(612..3074)
                     /locus_tag="VFV87_13125"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5104754.1"
                     /translation="MRTPMLMSLVGLIAALQAGFAEDLPVISVEGQPLAANVRRVVQA
                     LDLMGQPLPSEVAAKLEAAAKERDHAVLQSALDKHVLLAVAINPESRVKVVRGPAEAK
                     LQQGGYTPVIVKIVNEGAITKRLKIVSPQAGPVYAGAAQGSLLRQQQTELGENPNAAG
                     DKERFLGVEMFAAPPLSDKLSGLEVEYMLALIYCHESGKREATIGFDVEQGNQDLGFR
                     GETAVLFDVANAYPITVVADDDFEPTIVKMLIRDEQGHVYPPQAKRLAPDLFFQPHIY
                     RASNETILLPPGKFTVSYSRGPEYRVVQRPLTVKGPGVIDIDLKRWVDPAFHGFYSGD
                     HHIHAAGCAHYTSPTEGVLPEHMFRQVKGEGLNVGCVLTWGPCYKYQRQFFQERPHDL
                     SEPLTLLKYDIEISGFGSQALGHVCLLNLKDQTYPGSEGTETKGWPSWATPAMRWCKE
                     QGGVTGYAHSASGLWIDEKSASERMLTRYDADKSGELSAGELAGALLPEPFERIDADK
                     SRQVNLSELMASHARAAEQLPNLAIPEMNGAGAMELPVSVAEGVCDFISAMDTRRIQE
                     WNTWYHVLNCGFPLKASGETDFPCMSSRNVGQGRVYVRLGDVERIDYTAWCNGIAAGR
                     SYVSDGYAHALEFAVNKVRPGEGEVRLTQPGNVSILAKVAFAEETPLAVAHGGIIPPA
                     GKSVVGDTVVLHGPRSEEMLVGGKRLVELVINGVPAASREVPADGNEHDLQFEVPIAK
                     SSWVALRQFPQLHTNPVNVVVADRPIRASADSARWCIEAIDLLWKNREKNIKPQERAE
                     AQAAFDRARERYRQIAAEASEG"
     gene            complement(3145..>4133)
                     /locus_tag="VFV87_13130"
     CDS             complement(3145..>4133)
                     /locus_tag="VFV87_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5104755.1"
                     /translation="IGSCYTTRALVEGSLQLLKEHLLGESALEPERVSEKLRQSMFWL
                     GRGGSVEHTISGIDIALWDLLGKALNQPVARLLGGYYRDCIKPYASILFDDPPVLQRK
                     LEEQTSRGFKAIKMGWRPFGRVSRQLDEQLMKVARKTVGPDVELMVDAGGSEQFWPHN
                     VGWARETARMLGDYGVTWFEEALRPDDFDGFAELKANSPVLIATGEVLTRRQSFQPLI
                     DRRAVDIIQPDLTKCGGLSEGRKLGWAAYDHGVLLVPHGWNTAVGVAADLALTAALPA
                     ARWVEYQTGVPYIEEIIERPFALDADGLLQIPTGPGLGITLNREAIARFSPK"
BASE COUNT          674 a         1389 c         1303 g          767 t
ORIGIN      
        1 tgcttcgctc ggcgaaaggg cgttcgttcg cggagcgaac gacgacatta caccaccgcc
       61 tgccggtagc agtgctcaaa cagcagccgc cattcgaggc gggcgatctc gcgggcgagt
      121 tggtcggatt gggatttgcc gcgggcgatc gcttcgcaca gatcgacgaa gccaaatgcg
      181 tcccacgttt tctgcgcgac cagaaattcc gcagccgtat cgagctggtt ggcacgggcg
      241 agttgggcgg cctgctcggc cagcggtcca tggatcggat gttgcggcac gcggcggaac
      301 cagtacttgg cgttgccata gtccggctcg cggcggtgca tgattccgtg ccagtagctg
      361 ccgtccaccg tgtcgatttc ctggctgagc gcgtgcgact cgtccagaaa atcgtgccac
      421 agccacagcg ccgagaggca gcagcgggcg gcatcctgat cgacgactcg cttgttcgcg
      481 aatagcgatt cgacgctcag tgcggcaagc ttgggccgca ttgcttcgct cggcgaaccc
      541 ggccccagct ccgacagccg atcggtaatc aagtctgcga taggcggtgg atacgatacg
      601 ttcggcattt cctatccctc actcgcctcc gccgcaatct gacgatacct ctctcgcgcc
      661 cggtcgaacg cggcttgcgc ttcagcccgc tcctggggct tgatgttctt ctcgcggttc
      721 ttccacaaga gatcgatcgc ctcaatgcac cagcgggcgc tgtcggcgct ggcgcggatc
      781 ggccggtcgg cgacgacgac gttcacggga ttcgtatgga gctgcggaaa ctgccgcaga
      841 gcgacccaac tgcttttcgc gatcggcact tcgaactgca ggtcgtgctc gttgccatcg
      901 gctgggactt cccgcgacgc ggccggcacg ccattgatga caagttcgac cagccgcttg
      961 ccgccgacga gcatttcctc cgagcgcggg ccgtggagca cgaccgtatc acccacgacg
     1021 ctcttgccgg ccggcggtat gattccgccg tgggcgaccg ccagcggcgt ctcttcggcg
     1081 aacgcgacct tggccaggat ggagacattg cctggctggg tgagcctgac ctctccttcg
     1141 cccggccgca ctttgttcac ggcaaattcc agcgcatgcg cgtagccgtc ggagacgtac
     1201 gaccgtccgg ccgcgatgcc gttgcaccag gccgtgtagt cgatccgctc cacgtcgccc
     1261 aggcggacat acacccggcc ctggccgacg ttccggctgc tcatgcaggg aaagtcggtc
     1321 tcgccgctgg ccttcagggg gaagccgcag ttcaggacgt gataccaggt gttccattcc
     1381 tggatgcggc gtgtatccat cgcgctgatg aaatcgcaga ccccttcggc cacgctcacg
     1441 ggcaattcca tcgccccggc gccgttcatt tcgggaatgg cgaggttcgg caactgctcc
     1501 gcggcgcgcg cgtgcgacgc catcagctcg ctcaagttga cctgccggct cttgtcggcg
     1561 tcgatccgct cgaaaggttc gggcaacagc gcgccggcca gctcgccggc cgaaagctcg
     1621 ccgctcttgt cggcgtcgta gcgggtgagc atccgctcac tggccgattt ttcgtcgatc
     1681 cacaggccgc tggcagaatg ggcgtagccc gtgacaccac cctgctcctt gcaccaccgc
     1741 atggccggcg tggcccagct tggccaaccc ttggtttcgg tcccttcgga gcccggatac
     1801 gtctgatcct tgagattcag caggcatacg tgccccagag cctgcgatcc gaagccgctg
     1861 atttcgatgt cgtacttgag cagcgtcagc ggctcgctca ggtcgtgcgg ccgctcctga
     1921 aagaactgcc gctgatactt gtagcacggg ccccacgtca gcacgcagcc gacgttgagt
     1981 ccctcgcctt tgacctggcg gaacatgtgc tccggcagaa cgccctcggt tgggctggtg
     2041 tagtgggcgc agccggcggc gtggatgtgg tggtcgccgc tatagaaacc gtggaaggcg
     2101 ggatcgaccc atcgtttgag gtcgatgtcg atgacccccg ggcccttcac cgtgagcggt
     2161 cgctggacga cgcgatactc gggtccacgg ctgtacgaca ccgtgaactt gcccggcggc
     2221 agcaagatgg tctcgttcga cgcccggtaa atgtgcggct ggaagaacag gtcgggcgcg
     2281 agtcgtttgg cctggggcgg ataaacgtgg ccctgttcgt cacgaatcag catcttgacg
     2341 atcgtcggct caaaatcgtc atctgccacg accgtgatcg gataggcgtt cgccacgtcg
     2401 aacagcaccg ccgtctcgcc gcgaaagccg agatcctggt tcccctgctc gacatcgaag
     2461 ccaatcgtcg cctcgcgttt gccgctctcg tggcaataga tcaaggcgag catgtactcg
     2521 acttccaggc cgctgagctt gtccgacagc ggcggcgcgg cgaacatttc gacgccgagg
     2581 aaccgctcct tgtcaccggc cgcgttcggg ttctcgccca attccgtctg ctgctggcgg
     2641 agcaagctcc cctgcgccgc gccggcatag accggtccgg cctgcgggct gacgatcttc
     2701 agccgcttgg tgatcgcccc ctcgttgacg atcttcacga tgaccggcgt gtagcccccc
     2761 tgctggagct tcgcctccgc cggcccgcgc acaactttca cgcgcgattc cggattaatc
     2821 gccaccgcca gcagcacgtg cttgtccagc gccgactgca gcaccgcatg atcccgctcc
     2881 ttcgccgccg cctccagctt tgcagctact tccgaaggca gcggctgccc catcaaatcc
     2941 agcgcctgta ccactcgccg cacattcgcc gccaacggct ggccctccac cgaaatgaca
     3001 ggcaaatctt cggcgaaccc ggcctggaga gcggcaatca gaccaacgag cgacatcaac
     3061 atgggcgtgc gcatcgggtg aacctccggc tcgattcgcg tccgaatccg cctccatgtt
     3121 aaccgctgcc agcgaccctc gcggctattt cggcgaaaac cgggcgatgg cttcgcggtt
     3181 cagcgtgatg cccaggcccg ggccggtggg aatctgcagg aggccgtcgg catccaaggc
     3241 gaagggccgc tcgatgattt cttcgatgta ggggacgccg gtttgatatt cgacccagcg
     3301 agcggcgggc agggcggcgg tgagggccag gtcggccgcg acgccgacgg ccgtgttcca
     3361 gccgtgcggc accagcaaca cgccgtggtc gtacgctgcc cagccgagct tccgcccttc
     3421 ggaaaggccg ccgcacttgg tcaggtcggg ctggatgata tcgacggccc ggcggtcgat
     3481 cagcggctgg aacgactgcc ggcgggtgag cacctcgccc gtcgcgatca gcacgggcga
     3541 atttgccttc agctccgcga agccgtcgaa atcgtccggc cggagggcct cctcgaacca
     3601 ggtcacgccg tagtcgccga gcatgcgggc cgtttcacgt gcccagccga cgttgtgcgg
     3661 ccagaactgt tcgctgccgc cggcatcgac catcagctcg acgtccgggc cgaccgtctt
     3721 gcgggcgacc ttcatgagct gctcgtcgag ctgccggctg acgcgcccga acggccgcca
     3781 gcccatcttg atcgctttga agccgcggct ggtctgctct tccagcttgc gctgcagcac
     3841 gggcggatcg tcgaacagaa tcgaggcgta tggcttgatg cagtcgcggt aatagccgcc
     3901 gaggaggcgc gcgactggct gattgagggc cttgccaagg aggtcccaca gcgcgatgtc
     3961 gatgccgctg atcgtgtgct cgacgctccc gccgcggccg agccagaaca tgctctggcg
     4021 cagcttttcg ctgacgcgct ccggctccag agccgattcg ccaagcaggt gctctttgag
     4081 caactgcagc gagccttcga cgagcgcccg cgtcgtgtag caactgccga tgc
//
LOCUS       DASUGB010000687         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_354722, whole genome shotgun sequence.
ACCESSION   DASUGB010000687 DASUGB010000000
VERSION     DASUGB010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354722"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VFV87_13135"
     CDS             <1..1571
                     /locus_tag="VFV87_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5104756.1"
                     /translation="PPHHDIYSIEDLKQLIHDLKNANPSARVSVKLVSEVGVGTIAAG
                     VAKAYADHILISGDTGGTGASPITSIKHAGLPWELGIAETHQTLVMNDLRSRVTLQTD
                     GQIKTGRDVVIAALLGAEEIGFSTGPLVTLGCIMMRKCHLNTCPVGIATQDPVLRQKF
                     AGQPEHVINFLFLVAEEARQIMAQLGFRTFNDMIGRVDCLETNAAIKHWKAEGIDLTP
                     VLTPAPKPNPDVEVYCTRKQDHGLQDALDNELIADCQRAIKRREPVVLHKTIRNINRT
                     VGTMLSHEIAKRWGDELLPDDTIRIKFTGSAGQSFGAFLAEGITMELEGDANDYVGKG
                     LSGGRIAIYPPKTSSFRPEENILIGNVALYGATSGQAFFRGRAAERFAVRNSGAHTVI
                     EGVGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGIAYVWDPEGDFLTRCNLGIVELEKV
                     ETPQDLGELLHLIELHRDYTESTVAEKILAHWPEITTQFVKVMPVDYKRVLAERAKHD
                     EEVEAGVHGVPNHG"
     gene            1564..>2295
                     /locus_tag="VFV87_13140"
     CDS             1564..>2295
                     /locus_tag="VFV87_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5104757.1"
                     /translation="MGKPTGFKEFPRKPVPYRDPLARLADFGEIFTQPPEDHLRLQGA
                     RCMDCGVPFCQSASGCPIDNLIPEWNDLVYHGRWRDALDRLHKTNNFPEFTGRTCPAP
                     CEGACVLGITDPPVTIKNIENAIIDRGFEEGWVVAQPPPKRTGKKVAIIGSGPSGLAA
                     AAQLNKVGHSVTVYERSDRVGGLLMYGIPNMKLDKGVVARRVNLLREEGVEFVTNADV
                     GNRESPNFVDPQELLAKCDALLLATG"
BASE COUNT          454 a          741 c          731 g          369 t
ORIGIN      
        1 cgccgccgca ccacgatatt tactcgatcg aggacctgaa gcagctcatc cacgatctga
       61 aaaacgccaa tccatcggct cgtgtgagcg tcaagctcgt cagcgaggtg ggcgtcggca
      121 cgatcgccgc cggcgtggcc aaggcctatg ccgatcacat cctcatctct ggcgacacgg
      181 gcgggaccgg ggcctcgccg attacgagca tcaagcacgc cggcctgccg tgggagctgg
      241 gcatcgcgga gacgcaccag acgctggtga tgaacgacct ccgcagccga gtcacgttgc
      301 aaaccgacgg ccagatcaag acgggccgcg atgtggtcat cgccgcactg ctgggggccg
      361 aagagattgg tttttcgacc gggccgctcg tgacgctcgg ctgtatcatg atgcggaagt
      421 gccacctcaa cacctgcccg gttggcattg cgacgcagga ccccgtcctg cggcagaagt
      481 tcgccggcca gccagagcat gtcatcaatt tcctgttcct ggtggccgag gaggcccggc
      541 agatcatggc gcagctcggc ttccgcacgt tcaacgatat gatcggccgc gtcgattgcc
      601 tggaaaccaa cgccgccatc aagcactgga aggccgaggg gatcgacctc acgccagttc
      661 tcacgcccgc tcccaaaccc aaccccgacg tcgaggttta ttgcaccagg aagcaggacc
      721 acggcctgca agacgccctc gacaacgagt tgatcgccga ctgccagcgg gcgatcaagc
      781 gccgcgagcc ggtcgtgctg cacaagacga ttcgcaacat taaccgcacc gtcggcacaa
      841 tgctcagcca cgaaattgcc aagcgctggg gcgacgagct gctgcccgac gacaccatcc
      901 gcatcaagtt caccggctcg gccgggcaaa gctttggggc gttcctggcc gaagggatca
      961 caatggagct ggaaggggac gccaacgatt acgtcggtaa ggggctgtcc ggcggccgga
     1021 tcgcgattta tccgcccaag acctccagct tccggccgga ggagaacatc ctcatcggca
     1081 acgtggccct ctacggcgca accagcggac aggccttttt ccgcggccgc gccgccgagc
     1141 ggttcgccgt tcgtaacagc ggggcgcata cggtgattga gggcgtcggc gatcacggct
     1201 gcgaatatat gaccggtggc cgggtcgtcg tgctggggcc gaccggccgt aatttcgcgg
     1261 ccggcatgag cggcggcatc gcttacgttt gggacccgga aggagatttc ttgacccgct
     1321 gcaatctggg catcgtcgaa ctggagaagg tcgaaacgcc gcaggatctg ggcgagctat
     1381 tgcacctgat cgagctgcac cgcgattaca ccgaatcgac ggtcgccgaa aagatcctgg
     1441 cccactggcc ggagatcacc acgcagtttg tcaaagtgat gccggtcgat tacaaacgcg
     1501 tgctcgccga acgcgccaag cacgacgagg aggtcgaggc aggcgtgcac ggagttccga
     1561 atcatgggta aaccgacagg cttcaaagaa ttcccccgca agccggttcc ttaccgggac
     1621 ccgctggcga gattggcgga tttcggcgag atcttcacgc agccgcccga ggaccacctc
     1681 cgcctgcaag gggcgcggtg catggactgc ggcgtgccgt tctgccagag cgccagcggc
     1741 tgcccgatcg acaatctgat ccccgaatgg aacgacctgg tctatcacgg ccgctggcgc
     1801 gatgcccttg accggctcca caagacgaac aacttcccgg agttcaccgg ccgcacctgc
     1861 cccgcgccgt gcgaaggggc ctgcgtgctg ggcatcacgg atccgccggt cacgatcaaa
     1921 aacatcgaga acgcgatcat cgaccgcggc ttcgaggagg gctgggtcgt cgcccagccg
     1981 ccgccgaagc ggaccggcaa aaaggtggca atcattggtt ccgggccgtc gggcctggcg
     2041 gcggcggcgc agctcaataa agtcggccac agcgtcaccg tctatgagcg ctccgaccgc
     2101 gtcggcggcc tgctcatgta cggcattccg aacatgaagc tcgacaaggg ggtcgtcgcg
     2161 cggcgcgtga accttttgcg cgaagagggt gtcgagttcg tcaccaatgc cgacgtcggg
     2221 aaccgcgagt cgccgaattt cgtcgatccc caggaactgc tggccaagtg cgacgcgctg
     2281 ctgctggcga cgggc
//
LOCUS       DASUGB010000688         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_494529, whole genome shotgun sequence.
ACCESSION   DASUGB010000688 DASUGB010000000
VERSION     DASUGB010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494529"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFV87_13145"
     CDS             complement(<1..755)
                     /locus_tag="VFV87_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104758.1"
                     /translation="MRLALLAALMGILTAIDGAIGSPCAAAAEFPFTGYVATDAAEVA
                     AGAGRRFYVTDHLPLGAEVEVYREDDAGWLAIRPPAGSFSWIPAEHVERLAGDVGKVR
                     EPTESWIGTSVEQVKEHASQVSLKAGELVQILAEKKVGEGASQEHWLKIAPPAGEFRF
                     VHSRDISREPVASPPGSGEEVVVEEDGAAAGEAAAGEAEQHEPIRQPRRFRPSEGAIA
                     LRDIDEVRSRLAAMREDLAGLASRGPFAPQAGPR"
     gene            complement(877..2148)
                     /gene="fabF"
                     /locus_tag="VFV87_13150"
     CDS             complement(877..2148)
                     /gene="fabF"
                     /locus_tag="VFV87_13150"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5104759.1"
                     /translation="MKRRVVVTGLGVVTSLSCQVEDLFRRLLRGESGIHALRIFDTAA
                     FKVKFGGDVYDWNAEPWPHAFFARPDGFTEKKEIKRLDRFSQFAMVAGWQAVQESGLD
                     FAREDAFRCGVILGSGIGGLNEIEEGCAKILTKGPDRVSPFVVPKMMLNAAGGNLSIR
                     YGLRGVNYSVATACASANNAIGDAMKAIQYNELDVVITGGTESAITPMGLAGFQNMKA
                     LSTRNDDPPRASRPFDAERDGFVLSEGAGLLILEELEHAKARGAKIYCEVLGFGASAD
                     AGHITAPDEQGTGAARAMSDALASARLRPADIGYINAHGTSTPLGDKAETQAIKRVFG
                     DHARAVSISSTKSQLGHTLGASGGIELVITVKALAEGLIPPTINLTHPDPDCDLDYTP
                     NEARERRFTHAMSNSFGFGGHNASVVIGQVR"
     gene            complement(2197..2436)
                     /locus_tag="VFV87_13155"
     CDS             complement(2197..2436)
                     /locus_tag="VFV87_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5104760.1"
                     /translation="MASVAERVIDIVAEQLGVDKEKITPETSFVNDLGADSLDTVELV
                     MELEEEFDINIPDDAAEKIQTVGQAIKFIEEAQNT"
BASE COUNT          455 a          954 c          853 g          508 t
ORIGIN      
        1 cgcgggcccg cttgcggcgc gaaaggacca cggctggcca ggccggccag gtcctcgcgc
       61 atcgccgcca ggcggctgcg gacttcgtcg atgtcgcgca gggcaatcgc cccttccgac
      121 ggccggaatc gccgtggctg gcggatcggc tcgtgctgct ccgcttcgcc cgcggcagct
      181 tcgcccgcgg ccgcgccatc ctcctcgacg accacttcct cgccgctgcc gggcggagat
      241 gcgaccggct cgcggctgat gtcgcggctg tggacgaagc ggaactctcc ggccggcgga
      301 gcgattttca gccaatgctc ctggctggct ccctcgccca ctttcttctc ggccagaatc
      361 tgcaccaact cgcccgcttt cagcgagacc tggctcgcgt gctctttgac ttgctcgacg
      421 ctcgtgccga tccacgactc ggtcggctcg cggactttgc ccacgtcgcc agccagccgc
      481 tcgacgtgct cggccggtat ccagctaaag ctgcccgccg gagggcggat ggccagccag
      541 ccggcgtcgt cctcgcgata gacctcgacc tcggcgccga ggggcaggtg gtcggtgaca
      601 tagaaccgcc gccccgcgcc ggcggcaact tcggccgcgt cggtcgcgac gtagccggtg
      661 aacggaaact cggccgccgc cgcgcaaggg ctgccaattg ccccatcaat ggccgtcaga
      721 attcccatta gcgcagcaag aagtgccaga cgcatacagg ccccgcaatg cccccgccag
      781 gtagaaggaa acgcggcgac ttgtagcgaa tacttcgcgc gggctcaaga cggaagcggc
      841 ccgaacgcta gcttgcggcg cgggttaagc ctgctgtcat cgcacctgac cgatcaccac
      901 cgacgcgttg tggccgccga agccgaagct gttgctcatg gcgtgggtga agcgccgctc
      961 gcgggcctcg ttgggcgtgt agtcgaggtc gcagtcggga tcgggatgcg tcaggttgat
     1021 cgtcggcgga atcagccctt cggccagggc tttcacggtg atcaccaact cgatgccgcc
     1081 gctggccccc agcgtgtggc cgagctggct cttggtcgaa ctgatgctca ccgcccgggc
     1141 atggtcgcca aacacccgct tgatcgcctg ggtctcggcc ttgtcgccca atggcgtgct
     1201 ggtgccgtgg gcattgatgt agccgatgtc ggccggccgc agacgggcgc tggccagagc
     1261 gtcgctcatc gctcgggctg cgccggtccc ctgctcatcg ggggcagtga tgtgccccgc
     1321 atcggcgctg gcgccgaagc ccagcacttc gcagtaaatt ttggcccccc gcgccttggc
     1381 gtgctccagc tcttccaaaa tgagcaggcc agcgccctcg ctgagcacga agccgtcccg
     1441 ctcggcgtcg aacgggcggc tggcccgcgg cgggtcgtcg ttgcgcgtgg agagggcctt
     1501 catgttctga aagcccgcca gacccatcgg cgtgatcgcc gactcggtgc cgccggttat
     1561 cacgacatcg agctcgttat attgaatcgc cttcatcgcg tcgccgatgg cgttgttcgc
     1621 gctggcgcag gccgtggcga ccgaatagtt gaccccccgc aggccgtagc ggatcgacag
     1681 gttgccgccg gccgcgttga gcatcatctt gggcaccacg aacgggctga cccggtcggg
     1741 ccctttggtc aggatcttcg cgcagccttc ctcgatctcg ttcaggccgc caatgccact
     1801 gcccaggatc acgccgcagc gaaaagcgtc ttcgcgtgcg aaatcgagcc ccgattcctg
     1861 caccgcctgc cacccggcga ccatcgcgaa ctgcgagaac cggtcgagcc gcttgatctc
     1921 cttcttctcc gtaaagccgt cgggcctggc gaaaaaggca tggggccacg gctccgcgtt
     1981 ccagtcatag acgtcgccgc cgaacttcac cttaaaggcg gcggtgtcga agatccgcag
     2041 cgcgtgaatg ccgctttcac cgcgcaggag gcggcggaac agatcctcga cctggcaact
     2101 gagggaggtg acaaccccca gacccgtgac gacaacgcgc cgcttcatga gcatggccgc
     2161 ccgcgcccgg gcgcgggcca agacggtacc gcggggctaa gtgttctgcg cttcttcgat
     2221 gaacttgatc gcctggccga ccgtttggat cttttcggcg gcgtcatcgg ggatattgat
     2281 gtcgaattcc tcttccagct ccatgaccag ctcgacggtg tcgagcgagt cggcccccaa
     2341 atcgtttacg aacgacgttt ccggcgttat tttctcttta tcgacgccca attgctcggc
     2401 gacgatgtcg atcacgcgtt ctgcgacgga tgccactgtc accgtcctcc acaaaaagga
     2461 acccggctgc cgctaggccg accggcccgc tgtgcgccca aaaatcacga agtcgtacac
     2521 cgccacggtc aaccgctgat tgaatccaac aagatagagg atttccgcaa agggggtcaa
     2581 gtacggccga cgccggcaat gacgaatgtc gaaatccgaa tgacgaatga cccgcacctg
     2641 aatgttcaac cttcaattcc gtccttcggc tttcgtcatt cggatttcgt cattcggatt
     2701 tcgtcattcg tcctcctcct accccgtcat ccccccgtcc acggtcagca cttgcccggt
     2761 gacgtagcag
//
LOCUS       DASUGB010000689         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_910728, whole genome shotgun sequence.
ACCESSION   DASUGB010000689 DASUGB010000000
VERSION     DASUGB010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910728"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VFV87_13160"
     CDS             complement(<1..933)
                     /locus_tag="VFV87_13160"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX5104761.1"
                     /translation="MKRISLLLIGLVLLVAPRLVEAREGLTGPLADYVAAKDDSYAWL
                     KRSEGSVLGCQYVELTLTSQTWRGITWKHQLFVLKPAEINPAARHAVLLIGGGNWRDE
                     LADPKTEIKMPGEAQLLAIAAQQLKTPVAILLHVPQQPIFDGKREDEIIAYTFREFLK
                     SEDGTWPLLLPMVKSAVRGMDTVQTFAKQEWDLDVQHFTVTGASKRGWTTWLTGAVDD
                     RVTAIAPMVIDMLNMSPQIALQKDSFGELSEQIDDYEGLDKQINTPRGKALRKIVDPY
                     EYRDQLTQPKLIILGTNDRYWPLDAEKNYWPDLEG"
     gene            1217..>2480
                     /locus_tag="VFV87_13165"
     CDS             1217..>2480
                     /locus_tag="VFV87_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5104762.1"
                     /translation="MFATLLALIAGHAPAAPPGTTPVEGLRENTPAVYALTNVRIVPE
                     PGKAIEKGTIVIRDGVIEAAGADIKPPADARTIDLSGKTAYPGLIDAYSEITIGAEAR
                     RAGAPHWNPSIAPQLDVALHHVPDEALHGKLRSQGITARLVAPGSRIIKGQSIVIATG
                     EGDHARSILRSGVAQHFRLSVSPGGGREQYPNSPMGAVALARQTLLDADWYAKAWAAW
                     RGNNELPRPDRNDSLEALAPCLAGNQPAIIDAANEQYFLRADRFAREFGLQAIIRGSG
                     REYRRLDEIKASGRAVIVPVNFPQPPSVATVEDALDVSLQTLMHWDHAPENAARLDAA
                     GVPIALTSHGLKDPATFLSHVRRAIERGLSADAALKALTTTPADLLGIGERHGKIAPG
                     MAASLVIADGDLFAKKSKVVETWVEGRRY"
BASE COUNT          434 a          812 c          803 g          431 t
ORIGIN      
        1 gccttccagg tccggccaat agttcttctc cgcatccagc ggccagtagc ggtcgttggt
       61 gcccaggatg atgagcttcg gctgcgtcaa ctggtcgcgg tattcgtacg gatcgacaat
      121 cttccgaagg gccttgccgc gaggcgtgtt gatctgcttg tcgaggcctt cgtagtcgtc
      181 gatctgctcc gacagttcgc cgaacgaatc cttttgcagg gcgatctgcg gcgacatgtt
      241 gagcatgtcg atcaccatcg gagcgatcgc cgtcacgcgg tcatcgaccg ccccggtcag
      301 ccatgtcgtc cagccgcgtt tgctcgcccc ggtgaccgtg aagtgctgca cgtccaggtc
      361 ccactcctgt ttggcaaagg tttgtaccgt gtccatgccg cggacggcgc tcttcaccat
      421 cggcaacagc agcggccagg tgccgtcttc gctcttcaaa aactcgcgga acgtataggc
      481 gatgatctcg tcctcccttt ttccgtcaaa gatcggctgc tgggggacat gcagcaggat
      541 ggcgacgggc gttttgagct gctgggcggc gatagccaga agctgggcct cgccgggcat
      601 cttgatttcc gtctttggat cggccagttc gtcacgccag ttcccgccgc cgatcagcag
      661 cacggcgtgc ctagccgcgg gattgatctc ggccggcttc aggacgaaga gctggtgctt
      721 ccaggtaatg ccgcgccagg tctgcgacgt gagcgtcagc tccacgtatt ggcaccccag
      781 cacgctcccc tcgctccgct tgagccacgc atagctgtcg tccttcgcgg cgacgtaatc
      841 ggccagcggg ccggtcaagc cctcgcgggc ctccaccagc cgcggggcaa caagcagaac
      901 caaaccaatc agcagcagcg agatccgttt catggggagt cctgagagcg aaaggaaagg
      961 cgactcgact atcctaatcg actttgctcg gcggtcatac cgcagccggc ccgcagtggt
     1021 ttcagccgca ttttcacggc caaaccgtgc aggcgcctgc gggccggatt agacttgcag
     1081 gaccgtgccg tgggacgaag ccgtgcttcg tccacgcaat tcgaggagcg gacgaagcat
     1141 ggcttcgtcc tacaaccctg ccccaggatc gcccgcaatg ccgctcccaa gatccgtgcg
     1201 ctctgagcaa ctgctgatgt tcgccaccct tcttgccttg attgcgggcc acgccccagc
     1261 agcgccgccg ggcacgacgc cagtcgaagg actccgggag aatacgcccg cggtctatgc
     1321 actgaccaac gtgcggatcg tgccggagcc cgggaaggct atcgagaagg ggacgatcgt
     1381 catccgcgat ggcgtgatcg aagcggctgg cgcggatatc aagccgccgg ccgatgcccg
     1441 cacgatcgac ctttccggca agacggcgta tcccggtctg atcgatgctt acagcgagat
     1501 caccatcggg gcggaagccc gccgggccgg cgcgccgcat tggaatccga gcatcgcccc
     1561 gcagctcgat gtggccctgc atcatgtgcc cgacgaagcg ctccacggca agctgcggag
     1621 ccagggcatc accgcccggc tggtggcgcc cggctcgcgg atcatcaaag ggcagagcat
     1681 cgtcatcgca accggcgaag gcgatcacgc ccggtcgatc ctgcgcagcg gcgtggccca
     1741 gcactttcgc ttgagcgttt ctcccggcgg cggccgcgag cagtatccaa actcgccgat
     1801 gggagcggtc gccctcgccc gacagacgct cctcgatgcc gattggtatg ccaaggcctg
     1861 ggcggcgtgg cgggggaata acgagcttcc gcggccagac cggaacgatt cgctggaagc
     1921 gctcgccccg tgcctggccg gcaatcagcc ggcgatcatc gatgccgcca acgagcagta
     1981 cttcctgcgg gccgatcgct tcgcccgtga attcggcctg caggcgatca ttcgcggctc
     2041 cgggcgggaa taccgccggc tggatgagat caaggcgagc ggccgggcgg tcatcgtgcc
     2101 ggtcaatttc ccgcagccgc cgagcgtggc gacggtcgag gatgcactcg atgtgtcgct
     2161 gcagacgctg atgcactggg atcacgcacc ggaaaacgcg gcgcgtctcg acgcggcggg
     2221 agtgcccatc gcgctgacca gccacggcct gaaagacccg gcgacgttcc tgtcgcacgt
     2281 ccggcgggcc atcgaacgcg gcctttctgc tgacgcggca ctgaaggcgc tgacgaccac
     2341 accggcggat ctgctgggaa tcggcgagcg gcatggcaag atcgcgccgg ggatggcggc
     2401 gagcctggtg atcgccgacg gcgacctctt cgcgaagaaa agcaaggtcg tggaaacctg
     2461 ggtggaaggc cggcggtacg
//
LOCUS       DASUGB010000690         6868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_910732, whole genome shotgun sequence.
ACCESSION   DASUGB010000690 DASUGB010000000
VERSION     DASUGB010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910732"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..646
                     /locus_tag="VFV87_13170"
     CDS             341..646
                     /locus_tag="VFV87_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104763.1"
                     /translation="MLKSFLIGMCAMVAVAVLAQTAKAAEDKTHEGLVVSVADGKLTM
                     SDKDGKNEHTHNIAATTRITLDGKAAKLTDLKKGDKVKVTVGADGKVSAIEGSRAAG"
     gene            814..1305
                     /locus_tag="VFV87_13175"
     CDS             814..1305
                     /locus_tag="VFV87_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5104764.1"
                     /translation="MKMKTLDDLLVHELKDLLSAEKQLVKALPKMAKAATNPELKDAI
                     ENHLGETHGHVERLEKVFGLLDITSRGPKCQAMEGLVEEGKTVLEEEMDDDVRDAAII
                     AAAQRVEHYEIAGYGCARAFAERLGHDKVASLLQQTLDEEKTADVKLTEIAVQSVNEE
                     ALA"
     gene            1497..1664
                     /locus_tag="VFV87_13180"
     CDS             1497..1664
                     /locus_tag="VFV87_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX5104765.1"
                     /translation="MLYWALMFLIVALVAAVLGFGGIAGTMAWMAQVLFVVFLVLFLV
                     SLVAGTRAPPA"
     gene            1891..2307
                     /locus_tag="VFV87_13185"
     CDS             1891..2307
                     /locus_tag="VFV87_13185"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5104766.1"
                     /translation="MVNQQTLQGNWNEIRGRLRSKWGQLTGDDLQTFNGNVDQLVGLI
                     QRKTGEARSSIESFLEELTSNGASSIGQAAESVREYATQFAHETSKRAGEAAQTVRES
                     YEQAEDMIRQRPGESAAVCFGAGVLVGVLLGLVLRR"
     gene            2473..2748
                     /locus_tag="VFV87_13190"
     CDS             2473..2748
                     /locus_tag="VFV87_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104767.1"
                     /translation="MVAHEGTHRFRETLQRRIRHPEAMIENYPISTMLFAFGLGMGLG
                     IVIVQACGPTLMNLAYQEPSTSEKLSRQIYEAVSSVLPESISRRMHS"
     gene            2821..3258
                     /locus_tag="VFV87_13195"
     CDS             2821..3258
                     /locus_tag="VFV87_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104768.1"
                     /translation="MSSSNPSADEIQREMQQVRVELREDMQVMVQSARELTDWTYYVR
                     TYPWACMGAAAAIGFLIVPPRTPVIRPDPKDLLELVKDQKVTVKMEEKRAAPSFAGSL
                     LRMAAGSLLQGGLAILTHQFDQYRQEQARRAASNGNTRGTVHD"
     gene            3430..3924
                     /locus_tag="VFV87_13200"
     CDS             3430..3924
                     /locus_tag="VFV87_13200"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5104769.1"
                     /translation="MVDQAQVKSTNGSAESPPRAVARSATEFIHDVLTLAELQGKLLV
                     VDFRSGVRKLLWPTIGLAVGCVLALCCVPLALAALALAIAESTTLTHSQALGLVLAGA
                     ILAAAILVAGAALYLRHSWTIFDRSRTELTRNVQWSKEMLRKLGRDAPRRATTFVAST
                     RSHN"
     gene            3956..4141
                     /locus_tag="VFV87_13205"
     CDS             3956..4141
                     /locus_tag="VFV87_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104770.1"
                     /translation="MDYRNDVGYAAEPTTKLSDYELHPLDDFVTYLREYARERPEVVA
                     LSCFAIGFILGWKLKPW"
     gene            4284..4748
                     /locus_tag="VFV87_13210"
     CDS             4284..4748
                     /locus_tag="VFV87_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104771.1"
                     /translation="MTTTAISEKILSETLARLETALLTPVIAGEFSGWVHSVQQASAT
                     LAVDLTSCLHTILHVRYREIVEADPEQSANVEKLMVGDEQLLEQLTRFHERLYALGNA
                     AEHVDKNEGKLEQQRHKVEEEGIALILHVKKQQAASSTWLEEAFYRDRGVAD"
     gene            complement(4758..5465)
                     /locus_tag="VFV87_13215"
     CDS             complement(4758..5465)
                     /locus_tag="VFV87_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104772.1"
                     /translation="MSEKLMDVLVIDVGGSSVKLWHTGHQEHRKFDSGKDLLPETMVA
                     QARATAQDWTYEALALGLPCRISAGRPVDDPQNLGPGWVGFNFAAAFELPVRIMNDAC
                     LQALGSYDGGRMLFLSLGTAVGSAFVADRLVLSLDIGRLRIGEDRLFELLGDKGFEKL
                     GPKKWQRYVLEILPVLKNALLADYLVLGGGNAKEVEELPDGVRRGHNRAVVEGGRRLW
                     EQLSDPAQPQSTEWLIL"
     gene            complement(5462..6205)
                     /locus_tag="VFV87_13220"
     CDS             complement(5462..6205)
                     /locus_tag="VFV87_13220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5104773.1"
                     /translation="MSTQHLQYRALATDYDGTLAHDSHVAEETIAALRRLRESGRKVI
                     VVTGREMPELESVFPEFGLCDGIVAENGALLVYPAEEREEILGEPVPPEFLEEVTRRG
                     IKPYSVGRVIFATWRPHEASLLEVIHNLGIEYHIVFNKRAVMVLPSGINKATGLARML
                     KRLKLSPQNVVGIGDAENDHAFLDSCAVAVAVDNALPSLKQHCDLVVSKDHGAGVVEL
                     IDRLLADDLQGLGVRRPRQQALVAHRDTE"
     gene            6376..>6868
                     /locus_tag="VFV87_13225"
     CDS             6376..>6868
                     /locus_tag="VFV87_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104774.1"
                     /translation="MDSCRTSARYLQAAVSLLLAAGFVEAADGPRKTEVAIVGEAFHI
                     NGKPTYEGRTWEGHKIEGLLLNSRMVQGIFDDLNPETAGGWAYPDTGKWDAERNTREF
                     IAAMPLWRKHGLLAFTINLQGGSPQGYSRDQPWHNSAITPQGELRPEYMARLARILDK
                     ADAL"
BASE COUNT         1405 a         2061 c         2139 g         1263 t
ORIGIN      
        1 gtgagaattg gtggattaca cccatcggga aacgaggttg gctggcctgg agcgaagctg
       61 gcgtcaggat gagcgacata ttgccgacgg ccgatgctgc tcgacagttt gtcgcctcgc
      121 ttggcagtgc aagcggccag gccaagagat agttctcaat ctgcgacgct ctgcaccact
      181 cggaatgctg gcgacaaccg ccaaatgggc gatgcggtgg cgacaaaccg acgcctcatc
      241 cggcgggggc cagttgggaa tgaaaagtgc tttcgggagg cggccgagag caagaccggg
      301 cggtcttgcg ttgtgtacct cattcggagg agagccagag atgctcaaga gtttcctgat
      361 cgggatgtgc gcgatggttg ccgttgctgt tctcgctcag acagccaagg ccgcggaaga
      421 caagacgcat gaggggcttg ttgtttccgt tgccgacggg aagctgacga tgtccgataa
      481 ggacggcaag aacgagcaca cgcacaacat cgcggcgacc acgcggatca ccctggatgg
      541 caaggccgcc aaactgaccg acctgaagaa gggcgacaag gtcaaggtga cggtcggcgc
      601 cgacgggaaa gtatctgcca tcgagggcag ccgcgcggca ggatagcttc gatcattccc
      661 gcgtcgagcc gcaccaccgg gcacggaatc gccggcccgg tggtgggcct cttcttccgg
      721 catactcttt gcctggaata ctttgcggca aatcgccccg gcaaggcatg gccttgccgg
      781 acgtctttta taacccgcag ggagttgcca gtgatgaaaa tgaaaacttt ggacgatctc
      841 ttggtccacg agctcaagga cctgttgagt gcggagaaac aactcgtcaa ggcgctgcca
      901 aaaatggcca aggccgcgac gaatcctgag ctgaaagacg cgatcgagaa ccacctcgga
      961 gaaacgcacg gccacgtcga gcgactggag aaggtattcg gcctgctcga tatcaccagc
     1021 cgcgggccca agtgccaggc gatggagggc ctcgtcgaag agggcaaaac cgttttggaa
     1081 gaggagatgg acgacgacgt tcgcgatgcc gcgatcattg ccgccgcgca gcgcgtcgag
     1141 cattacgaga ttgccggcta cggctgtgcg cgggcttttg ccgagcggct gggccacgac
     1201 aaagtcgcat cccttctgca gcagacgctg gatgaagaaa agaccgccga tgtgaagctc
     1261 acagaaatcg ccgtgcaatc ggtcaacgaa gaggcattgg cctgaggcgc cgcgacaaac
     1321 cgtcgcggca gggcgccgaa tgatgcggca tccgcggacg ccagaccgaa ttggaacgga
     1381 atatgcgtcc ttcggttctt cgggtttggc cagccaaaaa aacgaagtgt ttgttttcga
     1441 ccagcgtcgc tgtcgagttt ttcgaaatgt cggcgacgca acgaaaggag ccaatcatgt
     1501 tgtactgggc actaatgttc ttgatcgtgg cgcttgttgc ggccgtgctg ggtttcggcg
     1561 gtatcgccgg taccatggcg tggatggcac aggtgctgtt tgtcgtgttc ctcgtcctgt
     1621 tcctggtgag tttggttgcc ggaacgcggg cgccgccggc ttagctgccg gtctggtgga
     1681 gcgagaatga cggatctgga ggcgacgaac tgctgcggca gtcgtcgcct cctttttttt
     1741 ggacaggcag agcagcccac gcactcaaac gccgcctttg cgaccagagt ggcggcttgg
     1801 cacggcggtt gctaaaggtt tgcagtgatt ccggacaaca cacgaccgcg aatccaaaca
     1861 catcacccgc cgtgcaagga gcaatgtccc atggtgaatc agcagacctt acaaggaaac
     1921 tggaatgaaa ttcgaggccg gcttcgcagc aagtggggtc agcttaccgg cgacgacttg
     1981 caaacgttca atggcaacgt cgatcagctg gtaggcttga ttcaacggaa aacgggcgag
     2041 gcccgcagct cgattgagag ttttctcgaa gaactgacat ccaacggcgc ctcgtccatc
     2101 ggccaggcgg cggaaagcgt gcgggaatac gcgacgcaat tcgcccacga aacttcgaag
     2161 cgggctggcg aagcggccca gactgtccgc gagagctatg aacaggcgga agacatgatc
     2221 cgccagcgtc ctggcgaatc ggccgcggtt tgcttcgggg cgggagtgct ggtcggtgtc
     2281 ctgctgggac ttgtactccg ccgctagagg cgcgccgcag ccggcggaga caggggaact
     2341 tcaggcgtca aaacacacca tccaacctta aagattttgt gatgtcccgg gaagttcctc
     2401 tctctccgcc ggctgccgcg gcagtaatcc ccaacagaca attcaccacg tttaacgaat
     2461 taggaggctg aaatggtcgc tcatgaggga acgcaccgct ttcgcgagac gctgcaacgc
     2521 cggattcggc atccggaagc gatgatcgag aactacccca tctcgacgat gctctttgca
     2581 ttcggactgg gaatggggct gggaatcgtc atcgtccagg cctgcggtcc aacgctgatg
     2641 aacctggctt accaggagcc cagcaccagc gagaagctca gccgccagat ctacgaggcg
     2701 gtttccagtg tcttgcccga gtcaatcagt cgccgcatgc acagctagtg ctcacaacgg
     2761 agtcgttgcg ctggcacagg ccgcaggttg tgcggctact cccctggatg taccgctcgc
     2821 atgtcttcat ccaatccatc cgccgacgaa atccagcgcg aaatgcagca ggtacgcgtg
     2881 gagctgcgtg aggacatgca ggtaatggtt cagagcgccc gcgagttgac cgactggacc
     2941 tattacgtgc gaacgtaccc ttgggcgtgc atgggggcgg cggccgcgat tggctttctg
     3001 atcgtcccgc cgcgaacgcc ggtcattcgg cccgatccca aggacttgct ggaactggtg
     3061 aaggaccaga aagtcacggt gaagatggaa gagaagcggg ccgctccgag cttcgcggga
     3121 tcgctcttgc gaatggcggc cggttcgctg ttacagggcg gcttggcgat cctcacgcac
     3181 caattcgatc aataccgcca ggagcaggcc cgccgggccg cctccaacgg caacacacgg
     3241 gggaccgtcc atgattaatc gcgtcgtccc ctctctcgca gagtccgcgg ccggacgcgg
     3301 gcccgatgag cagcggcagc cggcaaattg gcgggacaac gtgccgtggt tggagacgat
     3361 cgaaaccttt attgctgagc acccaggcgc atgcctggcg tcggcctttg ttgtgggagc
     3421 agtcgtcgca tggtggatca agcgcaggtg aaatcgacca acggaagtgc ggagtctccg
     3481 ccgcgcgcag tagcccgcag cgcgaccgag ttcatccacg atgtgctcac gcttgccgag
     3541 ctgcagggca agctgctggt cgtcgacttt cgctcaggag tgcggaagct tctgtggccg
     3601 acgattggcc tggccgtcgg ctgcgtgctg gcgctgtgtt gtgtgccgct ggcgctggcg
     3661 gccctggcct tggccatcgc cgaatcgaca acgctgactc attcgcaggc gctaggactc
     3721 gtgctggcgg gcgccattct ggcggcggca atcctcgttg ccggggcggc cctgtatctg
     3781 cgccatagtt ggaccatttt cgaccgctct cgcactgagc tgacgcgcaa cgtgcaatgg
     3841 tcgaaagaga tgctcagaaa actgggtcgc gacgcgccgc gacgcgccac gacgttcgtt
     3901 gcaagtaccc gctcacacaa ctgaccctga agaaacccga ttcggagaac caaatatgga
     3961 ctatcgaaat gacgtgggct acgcggctga gccgacgacg aagctctcgg actacgagct
     4021 gcatccactg gacgacttcg tgacctacct ccgcgaatac gcccgcgagc ggcccgaagt
     4081 cgttgctttg tcctgctttg ccattgggtt catcctgggc tggaagctca agccctggtg
     4141 actggcagcc ttcacaccat cgctgctcat cgacaggatg tcattccgag gtttttggca
     4201 tgacattgtg gcacgcagat tgcaccccaa ggtattcgtt cgcaaccgat ccaatggtga
     4261 gtgccgacgg caaaagtgcc acaatgacga ctaccgcgat atccgaaaaa atcctgtctg
     4321 aaacgctggc caggctggaa acggctctct taacaccggt catcgctgga gagttttcgg
     4381 ggtgggtcca ctccgtgcag caggcatcgg ccacgctcgc cgtcgatttg acgagctgct
     4441 tgcacactat tttgcacgtc cggtatcgcg agatcgtcga ggccgatcct gagcaatcgg
     4501 ccaacgtcga gaagttgatg gtgggcgacg agcagctctt ggagcaactg actcgcttcc
     4561 acgaacggct gtacgccttg ggcaacgccg ccgaacatgt ggacaagaac gaggggaaac
     4621 tcgagcagca gcgccacaag gtcgaggaag agggaattgc cttgattctg cacgtcaaaa
     4681 agcagcaagc cgcctccagc acctggctgg aggaagcctt ttaccgcgac cggggcgtgg
     4741 ccgattagcg tttacgttta gaggataagc cattccgtgg attgcggctg ggccggatca
     4801 gaaagctgct cccacaggcg gcggccgcct tccacaaccg cccggttgtg tccgcgacgc
     4861 acgccgtcgg gaagttcctc cacctccttg gcattgccgc ctcctaatac caggtagtct
     4921 gccagcaggg cgttcttcag cacggggagg atttccagca cgtaccgctg ccacttcttg
     4981 ggcccgagct tctcgaagcc tttgtcgccc agcagctcga acaggcggtc ttccccgatc
     5041 ctcaggcgcc cgatgtcgag cgacaatacc agccggtcgg ctacgaaggc cgaacccacg
     5101 gcggttccca ggctgaggaa caacatccgc ccgccgtcat agctccccag tgcctgcagg
     5161 caggcgtcgt tcatgattcg cacgggaagc tcaaacgcag ccgcgaagtt gaaccccacc
     5221 cagccggggc cgagattctg cggatcgtcg accgggcggc cggcgctgat gcggcagggc
     5281 agccccagtg ccagcgcctc gtacgtccag tcctgcgcgg tcgcgcgggc ctgggcgacc
     5341 atcgtttccg gcaagaggtc cttcccggaa tcgaacttgc ggtgctcctg gtggcctgta
     5401 tgccagagct tgacggaact cccgccgaca tcgatcacga ggacatccat aagcttctca
     5461 ctcactcggt gtcgcgatgg gcaaccaggg cttgctggcg gggacgccgc acgcccaggc
     5521 cttgcaaatc gtcggccaac agtcggtcga tcagttccac gacccccgcg ccgtgatcct
     5581 tggacaccac cagatcgcag tgctgcttga gcgatggcag agcgttgtcc acggcaacgg
     5641 ccactgcgca cgaatcaaga aaggcgtgat cgttctcggc gtcgccgatg ccgaccacgt
     5701 tctgcggcga gagcttcaga cgcttcaaca tccgcgccag ccctgtcgcc ttattgattc
     5761 cactcggcaa gaccatgacg gcccgcttgt taaagacgat gtggtattcg attcccagat
     5821 tgtgaatcac ttccagcagc gaggcttcgt ggggccgcca cgtggcgaag atcacccggc
     5881 ccaccgagta cggcttgatg ccgcgtcgcg taacctcttc caaaaactca ggaggaacgg
     5941 gctcacccag gatttcttcc cgttcttccg ccggatacac gagtaaggct ccgttttcag
     6001 ccacgatccc gtcgcacagg ccaaactccg gaaaaaccga ctcgagctct ggcatctcgc
     6061 gccccgtcac gacgatcacc ttgcggcccg attccctcaa tcgcctgaga gcggcgatcg
     6121 tctcttcggc cacgtggctg tcgtgggcca gcgtgccgtc gtaatccgtc gccagcgctc
     6181 gatattgcag atgttgcgtg gacatgcgtg gaagtcaact cggcctggcg tccagaacaa
     6241 ctaacgcaaa cgccgtgccg tcagtcgtca gccccctcgc ggaaggacga ttgccgagca
     6301 actcgcccgg cgcgtatgct ggagcagcca ggcgaccatt gtttgacatc accgaccgaa
     6361 agggaagtgt gtgccatgga ttcttgccga acctctgcac ggtatcttca ggcagcagtg
     6421 agcctgctcc tggcggcggg attcgtggaa gcagcggacg gtccgcggaa gaccgaagtg
     6481 gcgatcgtcg gcgaggcatt tcatatcaac ggcaagccga cgtacgaagg ccgcacctgg
     6541 gagggacaca agatcgaggg gctcctgctc aacagccgga tggtgcaggg cattttcgac
     6601 gacctgaatc ccgagaccgc cggcggctgg gcctatccgg acacgggcaa atgggacgcc
     6661 gagcggaaca cgcgggaatt catcgccgcg atgccgctct ggcgcaagca cggcctgttg
     6721 gccttcacca tcaatttgca ggggggcagt ccgcaaggct attcccgcga ccagccgtgg
     6781 cacaattcgg cgatcacgcc gcagggcgaa ctgcggcccg aatacatggc ccggctcgcg
     6841 cggattctgg acaaggccga cgcgctgg
//
LOCUS       DASUGB010000691         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1049775, whole genome shotgun sequence.
ACCESSION   DASUGB010000691 DASUGB010000000
VERSION     DASUGB010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049775"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VFV87_13230"
     CDS             <1..1218
                     /locus_tag="VFV87_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5104775.1"
                     /translation="REKGKSAATCNGYLRSIKSFTRWLHRDRRFRIDPLATLEALNEA
                     TDRRRQRRELMPTELAQLIETTANRTLPEHRFIGPDRAMLYCLAVGTGFRASELRSLT
                     PGSFDLDGNPATVTVAAAYSKRRRDDVQPISRSLAGLIRSWLEGKESDERLFAHMPGD
                     TARMLRADLAAARDAWIDAAKDDDAERKRRAGSDFLAYRNAAGEVFDFHGFRHAFISA
                     VVSGGASVKVAQELARHSSPTLTIGRYSHTRLYDLEGALAALPTMRMPHGTGEPKPQT
                     LRATGTCGDAGPAHASHLPVSEANGKRLSRLEGAQQEAQHSGRESSQIATDGCDDGGR
                     GGNEDPDCEAGPKALRLAGLGEHAQGQADHCESGGHGARTRNPLRGTTFPVELAYCPL
                     PSRNVRRRTRLRS"
     gene            complement(1727..2425)
                     /locus_tag="VFV87_13235"
     CDS             complement(1727..2425)
                     /locus_tag="VFV87_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104776.1"
                     /translation="MPDTPRILVIEDEPEIRRFLRAALTANQMQADEAESGQAGIRAA
                     ASQPPDVILLDLGLPDISGFEVIAQLRGWSRVPIIVLSARGQEADKVAALDAGADDYL
                     TKPFGIGELLARLRVALRHSVRIEHAAISPIFVLGECEVDQAARRVKVSKDEVHLTPT
                     EYRLLTTLIQHAGKVVTHRHLLKEVWGPDCTHETHYLRVYVNQLRQKLGRDFRSSLIT
                     EPGVGYRLEMSVES"
     gene            complement(2458..>2823)
                     /locus_tag="VFV87_13240"
     CDS             complement(2458..>2823)
                     /locus_tag="VFV87_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5104777.1"
                     /translation="LTNLVENALRFAPPGSQIKIAAGTTSGKIWLEVMDEGPGIPPGD
                     EERIFEKFYRAPNQQPRSGTGLGLAICDGIVQLHGGTIRADNRQENGAVFRIELPQPP
                     QPTLPIELPSERAISDAAK"
BASE COUNT          577 a          811 c          889 g          546 t
ORIGIN      
        1 cgcgagaagg gcaagagcgc ggcgacctgc aacggctatt tacgttcgat caagagcttt
       61 acgcggtggt tgcaccgcga tcggcgcttc cgcattgatc cgttggccac gctggaggcc
      121 ttgaacgaag caaccgaccg ccgccggcag cggcgtgagc taatgcccac tgaactggca
      181 cagttgatcg agactacggc caatcggacg ttgcccgagc atcggtttat cgggcccgat
      241 cgcgccatgc tctactgttt ggccgtcggc actggattca gggcgagcga gctgcggagc
      301 cttaccccag ggtcgtttga cctggacggc aatccggcca cggtgacggt tgcggctgcc
      361 tattcaaagc gccgtcgcga cgacgtccag ccgatcagcc ggtcgctggc gggattgatc
      421 cgcagttggc tggagggcaa ggagagcgac gaacggttgt tcgctcacat gcctggtgat
      481 acggcgcgaa tgctgcgagc cgacttggca gccgcacgtg atgcttggat cgatgcggcg
      541 aaggacgatg acgcagagcg aaagcggcgg gcaggatctg atttcctcgc gtaccgcaac
      601 gccgccggtg aagtgttcga tttccacggc ttccgccacg cgtttatctc cgcggtggtt
      661 agtgggggag cgtcggtgaa agtggcgcaa gagctcgctc gtcactcgtc accgacgttg
      721 acaatcggaa gatactcgca cactcggctg tacgacctgg agggtgcatt ggctgccctt
      781 cccaccatgc gaatgccgca tggcaccggc gagcccaagc cgcaaacgtt gcgggcaacg
      841 ggcacctgcg gcgatgccgg accggcccat gcttcccatt tgccggtatc agaagcgaac
      901 ggcaagcggc tgagcaggct ggaaggcgcg cagcaggagg cgcagcactc ggggcgcgaa
      961 agctcgcaga tcgctacgga tggatgcgac gatggggggc gtggcggcaa tgaggacccc
     1021 gactgcgaag ccggcccgaa agccttacgg cttgcgggat tgggcgaaca tgcacaaggt
     1081 caggcggacc attgcgagag cggagggcac ggggctcgaa cccgcaaccc cttgcggggc
     1141 accacatttc cagttgaact ggcctactgt ccactaccgt ccagaaacgt ccgaaggcgt
     1201 acacggttgc ggagttaggg cgagacgcaa aatccgccaa cattcgccgc tgttggccgc
     1261 tgtccgcccg tttggctaca aattggctac agtagcaggc gcaggtgaaa tacccggagc
     1321 cgatcgcacc acctcccgcg cgaaactcga atcagcgcga agccggttag cagtgacctc
     1381 cggacgaaaa tatgatgcgg aaacaatgcc agttccgaat ggcacgcgat ctttatccat
     1441 ctatgacgtg agtgctgtaa acctctccag caacccatgc tacggcatcg gagatgcctc
     1501 ttgcattttg gccgcggcgt aaatggggac gattcagtcg attgtaaagg gccccgaaac
     1561 atgccgtaca gccaaacatt cgctaatgga tagacaggcc ggcgcgaaca ttgtcgcgtg
     1621 tgagattcgc tcccacattt gcggtcggtg tgcctcaccg aatcaagctt gaattactgc
     1681 aatacgaagt ctgccgatct cgcacgttct tgtgaagcgg gccgcactag ctttcaacag
     1741 acatctcgag tctgtacccc acaccgggtt cggtgatcaa tgacgagcga aagtcgcggc
     1801 ccagcttctg gcggagctgg ttaacgtaaa cacgcaagta gtgcgtttcg tgcgtgcaat
     1861 ccggccccca gacctccttg agtaaatgtc ggtgagtcac aaccttgcca gcgtgttgga
     1921 tcagcgtagt aagcagccga tattctgtag gtgtcaaatg aacttcgtct ttcgatactt
     1981 tgactcggcg agcggcctgg tccacttcac actcgccgag cacgaagatt ggcgagattg
     2041 ctgcgtgctc gatacgaact gaatgccgca gggcgacacg caggcgggcg agaagttcac
     2101 cgataccgaa aggcttagtc agatagtcgt cggctcccgc atcgagggcg gcgactttgt
     2161 cagcttcctg cccacgggcg gatagcacaa tgatcggcac gcgagaccag ccgcgtagct
     2221 gggctattac ttcaaagccg ctaatatcgg gcaggcccag gtcgagcagt atgacgtcgg
     2281 gcggctgaga ggccgcggca cggatgccag cctggccgga ttccgcttca tccgcctgca
     2341 tctggttggc cgtgagcgcg gcccgtagga acctgcggat ttcaggttca tcttcgatca
     2401 ctaggatgcg cggagtgtcg ggcacgggat gaagagttag gggacgggag gccggttcta
     2461 ttttgccgca tcgcttatgg cgcgctcgga cggcagctcg ataggcagag tcggttgcgg
     2521 aggttgggga agctcgatgc ggaacacagc cccattttcc tggcgattgt ctgcgcggat
     2581 cgtgccgcca tgcaattgga caatgccgtc gcaaatggcc aaaccgagtc ccgtgccgga
     2641 ccgaggttgc tggtttggtg cgcgatagaa tttctcaaag atccgctctt catcaccggg
     2701 cgggatacca ggcccctcat ccatcacttc aagccagatt tttcctgagg tcgttcccgc
     2761 agcgatcttg atctggcttc ctggcggtgc gaatcgcagc gcgttttcga cgaggttggt
     2821 caa
//
LOCUS       DASUGB010000692         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_772175, whole genome shotgun sequence.
ACCESSION   DASUGB010000692 DASUGB010000000
VERSION     DASUGB010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772175"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFV87_13245"
     CDS             complement(<1..267)
                     /locus_tag="VFV87_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104778.1"
                     /translation="MSRLETLLAARKLDGTVARPEAIRMPGPQAATGIATLDDVLQGG
                     WRRGEVSEIIGGPSSGRTGVVCATLAAATARGEIVGVVDAFDRLD"
     gene            478..1749
                     /locus_tag="VFV87_13250"
     CDS             478..1749
                     /locus_tag="VFV87_13250"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5104779.1"
                     /translation="MTLAAGSPAPEVEEPRGWHKRLGGALTHRNFRILWIAALVSTIG
                     TWMQGFAQSYLIYSLTASNFYLGLDSFLGQLPILLFMLIGGVVADRHDRRRLLTTSQY
                     IQAASALSLAALVATGHVSIAAIFMLSFMSGCGQAFGGPAYQAMIPSLVPRRDLPNAI
                     ALNSTQFNLSRVLGPVAGAVVLSWIGVVWCFTLNGLSFFVVIVALTMLHLAAHVPQVN
                     PRRIREELRSGLRYVRESSVISTLAVLAFVTTFLAAPTSTFFPAFATSVLKSDQTPAQ
                     RLSMLMAAQGLGAIVGALIIGSLGRFRHMGRALLGGQIVLGIFVAAFAASRSLPLSVL
                     LVFIYGICSMAVFSMSFSLVQLTAPDELRGRIVSIYMVALRGGFPLGGLVAGAFADRF
                     GAPRVMMVSAGLLAVMAATLLIRRHSTLFQL"
     gene            complement(1755..>2702)
                     /locus_tag="VFV87_13255"
     CDS             complement(1755..>2702)
                     /locus_tag="VFV87_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5104780.1"
                     /translation="YINGFHDAANSIATVVSARVLTPVQAVLMAAFFNFIAVLTFGTT
                     VAQTMGQGMINLSAVTYAVILAGLIGAIAWNLITWFLGLPTSSSHALVGGYAGAAVAK
                     AGFAAILVSGWTKTLLFIVLAPLIGLTVGLLVMVAVFWTFRESTPSRVDRWFRRMQLI
                     SQAAYSLMHGANDAQKTMGIITGALLTGGFIQTFEVPPWVEIACYSAIGLGTLSGGWR
                     IIKTMGTKITKLTPAGGFAAQTGAAVAVFTATQLGVPISTTHTITGAIVGVGATRRLS
                     AVRWGIAGRIVWAWILTIPASAAIGAAMYYVTRLFPALQ"
BASE COUNT          423 a          879 c          908 g          492 t
ORIGIN      
        1 atccagccga tcgaacgcat cgacgacacc cacgatttcg ccacgggctg tcgcggccgc
       61 cagcgtggcg cagacaacgc ccgtacggcc cgacgacggc ccgccgatga tttcggacac
      121 ctcgccgcgc cgccatccgc cctgcaagac gtcatcgagc gtggcgatac cggtggccgc
      181 ctgcggtccg ggcatgcgga tcgcttcggg ccgggccacg gtgccgtcga gcttgcgggc
      241 ggcaagcaag gtttcgagtc tggacaaggc cgccgacgcc attttcgcta aactttcgcc
      301 ccctcgcgga gagtataggg ggagagcaca gacgttgcaa ctggggaact ggggaactgg
      361 cgagctgggg aactggggga ctggggaaca acgggaagac gcgcacgggg aaacgacgcg
      421 aacgacagct ccccgctccc ccagttcccc agttccccag ttataaaatc tctccacatg
      481 actttggccg ccggcagccc tgctccggaa gtcgaggagc ctcgtggctg gcataagcgg
      541 ctggggggcg cgctcaccca ccgcaacttt cgcatcctgt ggattgccgc gctggtgtcc
      601 acgatcggca cctggatgca ggggttcgcg cagagctatc tgatctacag cctgaccgcg
      661 tcgaattttt atctggggct cgacagcttt ctcgggcagt tgccgatcct gcttttcatg
      721 ctcatcggcg gcgtcgtcgc cgatcggcac gatcgccggc ggttgctgac cacctcgcag
      781 tacatccagg cggcgtcggc attgagcctg gccgcgctcg tcgcgaccgg gcacgtgagc
      841 atcgccgcga ttttcatgct gtcgttcatg tccggctgcg gacaggcgtt cggcggcccc
      901 gcgtatcagg cgatgattcc gtcactggtg ccgcggcggg acttgccgaa cgccattgcg
      961 ctcaattcca cccagttcaa tctttcgcgc gtgctcggcc cggtcgccgg cgccgtcgtg
     1021 ctcagctgga tcggcgtggt ctggtgtttc acgctgaacg gcctctcctt ctttgtcgtc
     1081 atcgtcgcgc tgacgatgct gcacctggcg gcgcacgtgc cgcaggtgaa cccgcggcgg
     1141 atccgggagg agctccggag cgggctccgg tacgtgcggg agtcgagcgt gatttccaca
     1201 ctcgcggtgc tggcctttgt cacgaccttt ctcgccgcgc cgacgtccac gttctttcct
     1261 gcatttgcga ccagcgtgct gaaaagcgat cagacgcccg cccagcggct ctcgatgctg
     1321 atggcggcgc aggggctggg cgccatcgtc ggcgcgctga tcatcgggtc gctcgggcgc
     1381 ttcaggcaca tgggccgggc gctgctcggc ggccagatcg tgctgggcat cttcgtcgcg
     1441 gcgttcgcgg cgtcacgctc gctgcccctg agcgtgctgc tggtcttcat ttatggcatc
     1501 tgttcgatgg ccgtcttctc gatgagtttc tcgctggttc agctcacggc gcccgatgag
     1561 ctgcgcggcc gcatcgtgtc gatttacatg gtggcgctgc gcgggggctt cccgctcggc
     1621 gggctcgtgg ccggcgcgtt cgccgatcgt ttcggcgcgc cgcgggtgat gatggtcagc
     1681 gcgggcctgc tggcggtcat ggcggcgacg ctgctgatcc gccgccacag cacgctcttt
     1741 cagctctaga tccgttattg gagtgccggg aatagccgcg tgacgtagta catcgccgcg
     1801 ccaatcgcgg ccgaggcggg aatcgtgaga atccacgccc agacgatgcg gccggcgatc
     1861 ccccagcgga cggccgagag ccttcgcgtc gcgccgacgc cgacgatggc gccggtgatc
     1921 gtgtgcgtcg tgctgatcgg cacgccgagt tgcgtggcgg tgaatacggc gaccgccgcg
     1981 ccggtttgtg cggcgaagcc tcccgcgggc gtgagcttgg tgatcttggt ccccatggtc
     2041 ttgatgatgc gccagccgcc gctgagcgtg cccaggccga tcgccgagta gcaggcaatc
     2101 tcaacccagg gcggcacctc aaaggtctgg atgaacccgc ccgtcagcag cgcgccggta
     2161 atgatgccca tcgtcttctg ggcgtcgttc gcgccgtgca tcaggctgta ggccgcctgg
     2221 gagatcaact gcatgcgccg gaaccagcga tcgacccggc tgggtgtcga ctcgcggaaa
     2281 gtccagaaca cggccaccat gacgagcagg ccgacggtga ggccgatgag cggcgccagc
     2341 acgatgaaga ggagtgtttt tgtccagccg ctgaccagaa tcgccgcaaa tcccgccttg
     2401 gccaccgcgg cgcccgcata gccgcccacc agcgcatgtg aggagctggt cggcaggccg
     2461 agaaaccagg tgatgaggtt ccaggcgatc gcgccaatca ggccggcgag aatcacggca
     2521 tacgtcaccg cggacagatt aatcatcccc tggcccatcg tctgggccac ggtcgtgccg
     2581 aacgtcagca cggcgatgaa attaaagaag gcggccatca gcaccgcctg gacgggcgtc
     2641 agcacgcggg ccgacacgac ggtggcgatg gaattggcgg cgtcgtggaa tccgttgatg
     2701 ta
//
LOCUS       DASUGB010000693         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1188615, whole genome shotgun sequence.
ACCESSION   DASUGB010000693 DASUGB010000000
VERSION     DASUGB010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188615"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV87_13260"
     CDS             <1..204
                     /locus_tag="VFV87_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104781.1"
                     /translation="KLTAAMNGGVMFLYAMALVVMNRMRLPREIRIPNWRLAILLVTV
                     AFFGFFTVWAAYDVLAKLPAAWK"
     gene            complement(243..1979)
                     /locus_tag="VFV87_13265"
     CDS             complement(243..1979)
                     /locus_tag="VFV87_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104782.1"
                     /translation="MSHRLPTRLIMLALHGMLVACLSATARLHAQTGLPGTGQRPTLG
                     EVGKLPEDWNPGKFDRRTGRWLSESARNIKWIAELGTQTFGAPVVAGGKVFVGTNNNG
                     PAYIRRYPATTDLGCLLCFRETDGKFLWQHSSEKLPTGRVHDLPMQGIQSSPWVEGER
                     AWFVSNRGEVICLDTRGFEDEEDDGEKFVGLRVARENAQPDPHEADVVWRLDMMDKLG
                     ARQHNNSTCCVTVWKETLFVCTGNGVDDSHIQIPAPEAPSFAALDKTTGNVLWTSNLP
                     GKNIHHGNWSSPAVGTLGGVPQVIFNGGDGWTYSFHAEEFQGGQPTLLWKFDTNAKDA
                     KLELGGRGTRNEPIGVPVLHEDRVYITTGQDAEHGEGPACIWCLDPTRRGDVSAELAV
                     LSADRKTIVPYDRIQAVQPEKGQVAIANPNSAVVWKYVGYDLNSDGELDPQEQMHRTL
                     SPVVFAGELLFATDFSGIVHCLDRKTGRPHWTYDALAAIWSPPLVADDKVYVADEDGD
                     IAIFRTSADPLIAGQRPPTAEIAGRPARIAPVREINMLNSSYTPPTASKGVLFIASKD
                     HLFAIEARQPSP"
     gene            complement(2005..2835)
                     /locus_tag="VFV87_13270"
     CDS             complement(2005..2835)
                     /locus_tag="VFV87_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX5104783.1"
                     /translation="MPSPFPGMDPYLEIPHVWPDFHNSLAYAIRALLNRDMPKPFYAQ
                     LEVRPEVGIVEIDEHVERMVPDVTVLEPRTVPKHSSGAAVLESPRTDISKSYEVAAVV
                     EPIRHAYVEIRDTAQDHRLVTLIEIVSPSNKRAGKDRDAYLEKQQQVLASDASLVEID
                     LHRGGQRLLAGEGLQRSVQRHSPPADYLVDVSRAWNRDKWSFYPINIREVLPVIGVPL
                     REQIPEVPLDLQFAATRAYDEGPYARGAVDYSRSPSPPLRESDRDWARQLVVQSGLAE
                     "
     gene            2829..>3019
                     /locus_tag="VFV87_13275"
     CDS             2829..>3019
                     /locus_tag="VFV87_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104784.1"
                     /translation="MASRFSLVNFRFHSSVRRTGSLEFLPHAEKSTYPGVIMVQAGSF
                     DPGGSVFPGDVTLVPARGSV"
BASE COUNT          538 a          927 c          937 g          617 t
ORIGIN      
        1 aagctgaccg cggccatgaa cggcggtgtc atgttcctct acgccatggc cctggtcgtg
       61 atgaaccgca tgcgcctgcc gcgagaaatc cgcatcccca actggcggct ggcgatcctg
      121 ctcgtcaccg tcgcgttctt cggcttcttc acggtgtggg cggcgtatga tgtgttggcc
      181 aaactgccgg ctgcgtggaa gtgatgcacc tttgagcgtc tacctcggcg cgattccatt
      241 gctcacggtg agggctgtct ggcctcgatt gcaaacagat ggtccttgct ggcgatgaac
      301 aggactccct tggaagccgt cggcggcgtg taagacgagt tgagcatgtt gatttctcgc
      361 accggcgcga ttctcgccgg acggccggca atctccgcag tgggcgggcg ctggccggca
      421 atgagaggat cggccgaggt gcggaaaatg gcgatgtcgc catcttcgtc cgcgacatag
      481 accttgtcgt cggccaccag cggtggcgac cagatcgcgg cgagcgcatc gtaggtccag
      541 tgcggccggc cggtctttcg gtccaggcaa tgcacgatac ccgagaagtc ggtggcgaag
      601 agcagttcgc ccgcgaacac gacaggcgat aacgtgcgat gcatctgctc ctgtggatcc
      661 agttcgccgt cggaattcag atcgtagccg acgtacttcc aaacgacggc cgagttggga
      721 ttggcgatcg ccacttgtcc cttttccggc tggacggctt gaattcggtc gtaagggacg
      781 atcgttttgc ggtcggcact caaaacggcc aactccgcgc tgacatcgcc ccgcctggtc
      841 gggtcgaggc accagatgca tgcggggcct tcgccatgct cggcgtcttg gccggtggtg
      901 atatagacgc gatcctcgtg caacacgggc acaccgatcg gctcgttgcg cgtgccgcgc
      961 cctccaagtt cgagtttcgc gtctttggcg ttggtatcga acttccagag caacgtcggc
     1021 tggccgccct gaaactcctc ggcgtgaaag ctgtaggtcc atccgtcgcc gccgttgaaa
     1081 atgacctgcg gcacgccccc gagcgttccc accgcgggcg aggaccagtt gccgtggtgg
     1141 atattcttgc cgggcagatt gctcgtccac aagacgttgc ctgtcgtctt gtccagcgca
     1201 gcgaagctgg gcgcctccgg cgcgggaatc tgaatgtggc tatcatcgac gccgttgccg
     1261 gtgcagacaa acagcgtttc cttccagacc gtgacgcagc aggtggaatt gttgtgctgg
     1321 cgtgcgccca gcttgtccat catgtccagc cgccacacca cgtcggcctc gtgcgggtca
     1381 ggctgcgcgt tttcgcgggc gacacgcagg cccacgaact tttcgccatc gtcctcttcg
     1441 tcctcaaagc cgcgcgtgtc gaggcaaatc acttcgcccc gattgctgac aaaccaggcg
     1501 cgctcccctt ccacccaagg ggacgactga atcccttgca tcggcaaatc atgcactctt
     1561 cccgtcggca gtttctccga tgaatgctgc cagaggaatt tgccgtcggt ctcgcggaag
     1621 cagagcaagc agcccaggtc ggtcgtcgcc ggatagcggc gaatataagc aggcccattg
     1681 ttgttggtcc cgacgaatac cttgccgccg gctaccactg gcgcgccgaa ggtctgagtg
     1741 ccaagttcgg cgatccattt gatattgcgg gcggactccg agagccacct gccggtgcgg
     1801 cgatcgaact tgcctgggtt ccagtcctcc ggcagcttgc cgacttcgcc taacgtcggg
     1861 cgctggccgg tgccgggaag gcccgtttgg gcgtgcagac gcgcggtcgc gctgaggcat
     1921 gcaacgagca tgccatggag ggccagcata atcaggcgtg tggggaggcg atgggacatt
     1981 ggtactcgcc gaaggatccc ggcatcactc agcaaggccc gactgcacga caagctgcct
     2041 ggcccaatcg cggtcgctct cgcggagcgg cggcgaaggt gatcgcgaat aatccaccgc
     2101 gccgcgagcg taggggcctt cgtcgtaggc tcgcgtcgcg gcgaactgca ggtccagagg
     2161 cacttccgga atctgctcgc gcagcggaac gccgatcacc ggcagcactt cgcgaatgtt
     2221 gatcgggtag aagctccact tgtcgcgatt ccaggcccgg ctcacatcga ccagatagtc
     2281 ggcgggaggc gaatgacgct gaacggaccg ctgcaagcct tctcccgcca gcaatcgttg
     2341 cccgcctcga tgaagatcaa tctcgacgag actcgcgtcg ctggctaaga cctgctgctg
     2401 cttttcgagg tacgcatcgc gatccttgcc ggcgcgtttg ttggatgggc tgacgatttc
     2461 gatcagcgtc accaagcgat ggtcctgggc agtgtcgcgg atttctacat acgcatggcg
     2521 gattggttcc accaccgcgg caacctcgta ggacttcgaa atgtccgtcc gaggcgattc
     2581 gaggaccgct gctccggaag agtgcttggg aacggtacgc ggctccagca cggttacatc
     2641 gggaaccatc cgttccacat gctcgtcgat ttcgacaatc ccgacctcgg gccgcacttc
     2701 caactgggca taaaacggtt taggcatatc gcgattgagt agcgctcgaa tcgcgtaggc
     2761 cagcgagttg tgaaagtccg gccaaacatg cgggatttcc aagtacgggt ccatgcctgg
     2821 aaacggtgat ggcatcgcgc ttctccctag tcaacttccg ctttcattct agcgtgcgca
     2881 ggaccggttc actcgaattc ttgccgcatg cggaaaaatc aacataccct ggcgtcataa
     2941 tggtgcaggc ggggtcgttc gatcccggcg gatctgtttt ccctggcgac gtaactctcg
     3001 tgcctgcccg tggttctgt
//
LOCUS       DASUGB010000694         7821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2022845, whole genome shotgun sequence.
ACCESSION   DASUGB010000694 DASUGB010000000
VERSION     DASUGB010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022845"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VFV87_13280"
     CDS             <1..887
                     /locus_tag="VFV87_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5104785.1"
                     /translation="NKAAQNPGGKFPPGFGQDFSPDKFLEQLFGRAGVDLNDDAELEK
                     IEITPAEEARLGQQALDGVKNRLGQQKVKLLEKGQEVQYLSRLVELIQPQMTQAKRYR
                     KLQVYLADMDDPDACTLPGGHVIFSRGLLEAAGCEAALVCVAGHELAHLDRGHLLRRS
                     RQWKLAQQRMSAAPGEFSFDKLLQMNQTMMQLFRRPFGPEEELEADRDGITWAYRAGY
                     DPRALATIYQAFDAAGKNPPEFLPAFFRTHPLTAERRENLTQTFATLQAAEPKGDLYL
                     GRENLARRLTRAEREFAE"
     gene            891..1460
                     /locus_tag="VFV87_13285"
     CDS             891..1460
                     /locus_tag="VFV87_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104786.1"
                     /translation="MVEQPYSVDCECGKTLPVSKSDAGSEIRCRCGKSVSVPRLSLLR
                     RAKGEDALEAGIRDRIARMIREGKLPVGDCCAVSGFPTNDVMLFDVICERSYTKGARN
                     WGIALLVLGFFTPFSWFFWLLGYDMLNERTERVGRDVVLTVPLRVAKENQGKLRGRTS
                     QKRLQIVLGSVPEYELLFQEFPDARISAH"
     gene            1470..2474
                     /locus_tag="VFV87_13290"
     CDS             1470..2474
                     /locus_tag="VFV87_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5104787.1"
                     /translation="MPSRLFPLTTLSELADGQEADFFALLSAKSEATTRDGKPYWRVT
                     FRDALREVSFPLWSDSGFATACKDEWKVGECYKLRAIYAETKFGPQLELKKVRPVIES
                     DKADGFDPWSFLPRSKFDSAAMFQQLVGLAKSEIAEQSLRALTVELLEQNKEVLLRLP
                     AARRNHHPFVGGWLEHVLSVTRNAIFLADKYAADYPELQPPLARDVVVAGAILHDIGK
                     VHELSFSPAGAEYSPAGELIGHILIGRDMVREAAQRHPLSPETLLRLEHVVVSHQRLP
                     EWGSPKPPMTPEAILVHFADDVDAKFQTAMAALAEPGEGPLTSSRNPLQHRIFRGLGE
                     "
     gene            complement(2506..3771)
                     /locus_tag="VFV87_13295"
     CDS             complement(2506..3771)
                     /locus_tag="VFV87_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HEX5104788.1"
                     /translation="MTRSISIASVLGVVALLAAGASLCAQPSPSDRAPNDRGPAAEQP
                     WEEGYAASPCEYGACEYGACDCYGPPRITARAEYLMWWSRGRNVPPLVTTSPQTTPRD
                     EAGVLGFPTTTILYGDEPIGEDFRSGGRFSLSYLLATGCTWAEGRIWGLEDSSESFLA
                     ASNGDPILARPFFNALLGQEDALLVAFPGVTMGGSIQVRSKNDLFGADAWVRQVWCQD
                     CCIRLDVLAGYQFIRLDDALTINNVQTSIDPNSVFPFGTVIDNLDSFATRNEFHGGQL
                     GIAAEYCGCCWNIELLAKLALGGVHEEVAIAGRTITAEPGAAPVVTSGGLLAQPTNIG
                     RYQRGRLAIIPEVNVNLAYDVNPCWRLTLGYSFLYWSNAVLAGNQIDRAVNLSQVSGP
                     LVGPARPQFTFNRTDYWVQGMNFGLQYRW"
     gene            3992..5350
                     /locus_tag="VFV87_13300"
     CDS             3992..5350
                     /locus_tag="VFV87_13300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5104789.1"
                     /translation="MVAWVFLIGVFNIALGYLVAASLADPPFWAGWKRRWELLAARAA
                     KAGAQSPVEEAPTEPPPPAEPETASDSEANTLDPEPEPEPVVPATIAELPSHWLQQLA
                     ESGIVPKSYVEASAHVIRLEVGRYREQLLTAENRARANLATSDAGALKLVADDLRFVN
                     QDWLEKQTTAAGMLAQRSGRLGTHEQPAKAVEQSLLDQAAQIRLICTALEDLDFTEGA
                     EASGRRLLGMIGSLISSAHLLRDRMLDLLADLLRTGEQLEGLSPSVQLDYLTGLPNRV
                     GTELLFDAWWQENPERSRPLSVALIDVDRFGRVNHRMGTRGGDRAISALGRLLSELIR
                     KDRGYDRAGRVGGEKFLLLLGDTGPHNALAAAERIRQTVEATTFDDQGAEFEMTVSCG
                     VVEMGSAESLPDVLHRVEQAVTFAKRSGRNRCAIDEGAGATALPPPQLAVVGRVVHLS
                     AE"
     gene            complement(5354..7282)
                     /gene="mutL"
                     /locus_tag="VFV87_13305"
     CDS             complement(5354..7282)
                     /gene="mutL"
                     /locus_tag="VFV87_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX5104790.1"
                     /translation="MPAIRQLPPSVVNKIAAGEVIERPASVVKELLENAVDAGATRID
                     VALGQGGTDLIRIVDDGCGIARDDLLLAVSSHATSKIADADDLFHVATFGFRGEALAS
                     IAEVSQFTIRSRLQAAEAGYELEVVGGQRSEIVPCGCPAGTAIEVRNLFYNTPVRRKF
                     LRASQTEIAHCTEAFTRIALAQPQIHFTLRHNDKLLFDLPPTEHWRERIAAFFGRDLE
                     SSLIAIDNHDEGVRLLGFVADPQHSRANNRTQYLLLNGRFIRDRALQHALGEAYRGLL
                     LTGRFPIAFLRLEMPAEAIDVNVHPTKLEVRFADSGRIYSLLLGTIRKKFLATDLTAK
                     VRSPVGAEEAGAQAVDVAAAQQHRRELVDWAKGAIAALPRGEGAATESGEDASQVLLD
                     LRFERQGAAPLEITRLDRAWEAAPTSPRLQQAGEEAGRLAGHPLAGPQTPARADYSRA
                     PRSSHLGFQIHNRYLVTENDEGMVVIDQHALHERILYEQLREKVLAGEMETQRLLVPE
                     PVPLPPAEAAAALEAQETLAQLGIEVEPFGGDTVLVSSYPAMLANISPAEVLRQIVEE
                     LVSSSKQPERRDLLDELLHMIACKAAIKAGDRLAPEEITALLEQRHHYQDSHHCPHGR
                     PTALVFSRDELDRRFKRT"
     gene            complement(7315..>7821)
                     /locus_tag="VFV87_13310"
     CDS             complement(7315..>7821)
                     /locus_tag="VFV87_13310"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5104791.1"
                     /translation="TEDDSVELLEGLIVKKMTKNPLHDSTIDYLNTVLSRQLPPGWFP
                     RIQNVLRTEDSEPEPDVVIAIGDPTKFRRRHPTGEEVALVIEVAESSLEQDWRKCRIY
                     ARAGVAEYWIVDLNAARLEVFKQPNRALGKYESHVTLTAPGEAAVNLSATARLVVDLQ
                     ELLPPAGE"
BASE COUNT         1402 a         2494 c         2555 g         1370 t
ORIGIN      
        1 taaacaaagc ggcgcagaat ccaggcggca agtttccgcc gggctttggg caggactttt
       61 cgcccgacaa gttcctggaa cagctcttcg gccgcgccgg tgtggatctg aatgacgacg
      121 cggagctgga gaagatagaa atcacgcccg cggaagaggc caggctcggg cagcaggcac
      181 tggacggtgt gaagaatcgc cttggacagc agaaagtcaa actgctggaa aaagggcagg
      241 aggtgcaata cctgtcgcgc ctggtggaac tgatccagcc gcagatgacc caggcgaagc
      301 gctatcgcaa gctgcaggtc tatctggccg acatggacga tcccgatgcc tgcacgctgc
      361 cgggcgggca tgtgattttc tcgcgcgggc tgctggaagc ggccggctgt gaagcggcgc
      421 tggtctgtgt ggcaggccac gagctggcac acctcgaccg cgggcatctg ctccgccgca
      481 gcaggcagtg gaagctggcc cagcagcgga tgtccgccgc gcccggcgaa ttctcgttcg
      541 ataagctgct gcagatgaat cagacgatga tgcagctctt ccgccggccc ttcggcccag
      601 aagaggagct ggaagccgac cgcgacggca tcacctgggc ctaccgcgcc ggttacgacc
      661 cgcgggcgct ggcgacgatc tatcaggcct ttgacgccgc cggcaaaaac ccgcccgagt
      721 tcctgcccgc ctttttccgc acccatccgc tcaccgccga gcgccgcgaa aacctcacgc
      781 aaacctttgc cacgctgcaa gccgccgagc ccaaaggcga tctctacctc ggccgcgaaa
      841 acctcgcccg ccgcctgacc cgcgccgagc gggagtttgc ggagtaatca atggtcgagc
      901 agccctactc cgtcgattgc gaatgtggga agacgctccc tgtatcaaag agtgatgcag
      961 gctcggagat tcgctgtcgt tgcggcaaat cggtgagcgt cccccgatta agcctgttgc
     1021 gacgagccaa gggggaagat gcgctggagg ctgggattcg tgatcgaatc gcgaggatga
     1081 tccgtgaagg aaaactgcca gtaggtgatt gctgtgcggt ttcaggattc cccaccaacg
     1141 acgtcatgtt gttcgacgta atctgcgagc ggtcttatac caagggagcg cgaaactggg
     1201 gaatcgcatt gcttgttctg ggtttcttca cgcccttttc gtggttcttt tggttgctgg
     1261 gatacgacat gttgaatgag cgaaccgaac gagtcgggcg cgacgtcgtg ttgacagtcc
     1321 cgctgcgagt cgccaaggaa aaccagggaa agctgcgagg aaggacgtcg cagaagaggt
     1381 tgcaaatcgt gctcggcagc gtgcccgagt acgagcttct ttttcaagaa tttcccgacg
     1441 cacgaatctc cgcacattga ctccggacca tgccatcaag acttttcccg ctcacgacgc
     1501 tcagtgaact ggccgacggc caggaagccg attttttcgc gctcctgtcg gccaagagcg
     1561 aggcgacgac gcgggatggc aagccctatt ggcgggtgac gtttcgcgat gccttgcggg
     1621 aggtgagctt tccgctgtgg agcgattccg gcttcgccac ggcgtgcaag gacgagtgga
     1681 aagtgggcga gtgctacaag ctgcgggcga tttatgccga aacgaaattc ggtccgcagc
     1741 ttgagctgaa gaaggtccgc ccggtcatcg aaagtgacaa ggccgacggc ttcgatccgt
     1801 ggagctttct gccgcggagc aagttcgatt cggcggccat gttccagcag ctcgtcggcc
     1861 tggcaaagag cgaaatcgcc gagcagagcc tgcgggcgct gaccgtcgaa ctcttggagc
     1921 agaacaagga agtgctgttg cggctgccgg cggcccggcg gaatcaccac cccttcgtcg
     1981 gcggctggct ggagcacgtg ctgagcgtga cgcggaacgc gatctttctg gccgacaaat
     2041 acgcggccga ttacccggag ctgcagccgc cgctggcccg cgacgtggtc gtcgccgggg
     2101 cgattttgca cgacatcggc aaggtacatg agctgtcgtt ctcgcccgcg ggcgccgagt
     2161 acagcccggc gggcgagctg atcggccaca tcctgatcgg ccgcgacatg gtccgcgaag
     2221 cggcacagcg gcatccgctt tcgcccgaga cgctgctgcg gctggagcac gtcgtcgttt
     2281 cgcaccagcg actgcccgaa tggggctcgc ccaagccgcc gatgactccc gaggcgatcc
     2341 tcgtccactt tgccgacgac gtggacgcga aattccagac tgccatggcc gccctggccg
     2401 aacccggcga aggcccgctc accagcagcc gcaatccact gcagcaccgg attttccgtg
     2461 ggctggggga gtaactcgcg aggggtgata accgataagt cctcgttacc agcgatactg
     2521 caacccgaag ttcattccct ggacccagta atccgtccgg ttgaacgtga attgcggacg
     2581 ggccgggccg acgagcgggc cggagacctg cgaaagattg accgcccggt cgatctggtt
     2641 gccggccagc acggcgttgc tccagtagag gaacgaatag cccagcgtca gccgccagca
     2701 gggattcacg tcgtaggcga gattgacgtt gacctcggga atgatcgcca gccggccgcg
     2761 ctggtagcgg ccgatgttcg tcggctgggc gagcaggccg ccgctggtga cgacgggcgc
     2821 ggcgccaggc tcggcggtga tcgtgcggcc ggcgatggcg acctcttcgt gcactcctcc
     2881 cagagccagc ttggcgagta gctcgatatt ccagcagcag ccgcagtatt ccgcggcgat
     2941 gccaagctgc ccgccgtgga attcgttgcg cgtagcgaag gagtcgagat tgtcgatcac
     3001 ggtgccgaag gggaagacgg aattcgggtc gatcgacgtc tggacgttgt tgatcgtcag
     3061 cgcatcgtcg agccggatga actggtagcc ggccagcaca tccaagcgga tgcagcaatc
     3121 ctggcaccaa acctggcgga cccaggcgtc ggctccgaac aggtcgttct tcgaccgcac
     3181 ctggatgctg ccgcccatgg tgactcccgg aaacgcgacg agcaaggcgt cctcctggcc
     3241 gagcagtgcg ttgaagaacg gccgggccag gataggatcg ccattggacg cagccaagaa
     3301 cgattcgctg ctgtcttcca ggccccagat gcggccttcg gcccacgtgc agcccgtggc
     3361 cagcaggtag ctcagcgaga atcgcccgcc ggagcggaag tcctcgccga tcggctcgtc
     3421 gccgtacaga atcgtcgtgg tggggaaccc gagcacgccc gcctcgtcgc gcggcgtcgt
     3481 ctgcgggctg gtcgtcacca gcggcgggac attgcggccg cgactccacc acatcaggta
     3541 ttcggcgcgc gcggtaatgc ggggcgggcc gtagcaatcg cacgcaccat actcgcacgc
     3601 tccgtattcg caagggctcg cggcgtatcc ctcctcccac ggctgttcgg ccgcaggtcc
     3661 cctatcgttg ggcgccctat cgctgggcga aggctgggcg cagagcgaag cgccggctgc
     3721 caacagcgca accacgccaa gcacggaagc gatagagatc gatctggtca tgcggctttt
     3781 ccgacggcgc aagctctaac ggttgtatcg acgaaatggt atgtgaggat caacttgccc
     3841 gtccaaagcg aaaactggga agattgggaa gcatgtaacg attgagaaga cgtgggatgt
     3901 gctgtggcgg aagaccgcat gcggcgattc gaatggggcc gacccaggcg acgtaggttt
     3961 tgacgtgcca tgaccttgcg cggagaagac cgtggttgcg tgggtgtttt tgatcggggt
     4021 tttcaacatc gcgctcggct acctggtggc cgcgtccctg gctgatcctc cgttctgggc
     4081 tggctggaaa cggcgctggg aattgctggc agcgcgtgcg gccaaagccg gcgctcagtc
     4141 gccggtcgaa gaggccccca ctgagccgcc tccgcctgca gagccggaga ccgcgtctga
     4201 ttccgaggcg aacacgctcg atcccgaacc agaaccagaa cctgtcgttc ccgcgacgat
     4261 cgccgagctg ccatcgcact ggctgcagca gttggccgaa tcgggcattg tgccgaagtc
     4321 gtacgtcgag gcctcggccc acgtcatccg cctggaagtt ggccgctacc gcgagcagct
     4381 cttgaccgct gaaaaccggg cccgcgccaa tctggccacc agcgatgccg gggccctcaa
     4441 gctcgtcgcc gacgatttgc gattcgtgaa ccaggactgg ctcgaaaaac agaccactgc
     4501 agccgggatg ctcgcccagc gctcggggcg tctcggcacg cacgagcagc ctgccaaagc
     4561 ggtcgaacag tcactgctcg accaggccgc tcaaatccgc ctgatctgca ctgccctgga
     4621 agacctcgac ttcactgaag gcgccgaagc ctcaggccgg cggctgctcg gaatgatcgg
     4681 ctcgctgatc agctccgcgc atctgttgcg cgaccggatg ctcgaccttc tggccgacct
     4741 cttgcggacg ggcgagcagc tcgaagggct cagtcccagc gtgcagctcg actatcttac
     4801 ggggctgccc aatcgcgtcg gcaccgaact cttgttcgac gcctggtggc aggagaatcc
     4861 ggagcggtcg cgtccactca gcgtggcgct gattgacgtc gatcgcttcg gacgcgtcaa
     4921 ccatcgcatg ggaacccgtg gcggtgaccg ggccatctcc gccttgggac gcctgctgag
     4981 cgagctgatt cgcaaggacc gcggttacga ccgggccggg cgcgtgggtg gcgagaaatt
     5041 cctgctcctc ctgggcgata cgggcccgca caatgccctg gccgctgccg agcggattcg
     5101 ccagacggtc gaggccacga ccttcgacga tcaaggcgcc gagtttgaaa tgaccgtcag
     5161 ttgcggcgtc gtggaaatgg gcagtgccga gtctttgccc gacgtgctgc accgggtcga
     5221 gcaggcggtc acgttcgcca agcgctcggg ccgcaaccgc tgcgccatcg acgagggcgc
     5281 gggagcgacg gcgctccccc cgccgcagct cgccgtcgtc gggcgcgtcg tccatctcag
     5341 cgcggagtag gcctcacgtc cgtttgaatc gccggtccag ctcgtcacgg ctgaagacca
     5401 gcgcggtcgg ccggccgtgc gggcagtggt gcgaatcctg ataatggtgc cgctgctcga
     5461 gtagcgccgt aatctcctcc ggcgccagcc gatcgcccgc cttgatcgcc gccttgcagg
     5521 cgatcatgtg gagcagctcg tccaacaggt cgcggcgctc gggttgcttg ctcgacgaaa
     5581 ccagctcctc cacgatctgc cgcagaactt ccgccgggct gatgttggcg agcatcgccg
     5641 gatagctgga aacgaggacc gtgtcgccgc cgaatggctc gacctcgatg ccgagctgcg
     5701 ccagcgtctc ctgcgcttcc aaggcggcgg cggcttccgc cggcggcagc ggcacgggct
     5761 ccgggaccag cagccgctgc gtttccatct cgcccgccag gaccttctcg cgaagctgct
     5821 cgtagagaat ccgctcgtgc agggcgtgct ggtcgatgac gaccattcct tcgtcgttct
     5881 ccgtgaccag ataccggtta tggatctgaa aaccaaggtg ggagctgcgc ggcgctcttg
     5941 aataatcagc tcgcgccgga gtctgcgggc cggccagcgg atgccccgcc aggcggcccg
     6001 cttcctcgcc cgcctgctgc aggcgcgggc tggtgggggc cgcctcccag gcgcgatcga
     6061 ggcgcgtgat ttccagcggt gcggcgccct ggcgctcgaa ccgcaggtcg agcagcactt
     6121 gcgaggcatc ctcgccgctc tcggtggcgg cgccttcgcc gcgcggcagc gccgcaatgg
     6181 cccccttggc ccaatcgacc agctcgcggc gatgctgctg ggcggcggca acgtcaaccg
     6241 cctgcgcgcc ggcctcttcg gcgccaaccg gcgagcggac tttcgccgtc aggtcggtcg
     6301 ccaggaattt cttgcgaatc gtccccagca ggagcgaata gatgcggccg ctgtcggcaa
     6361 agcggacctc cagcttcgtc ggatgcacat tcacgtcgat cgcctcggcc ggcatttcca
     6421 gccgcaggaa ggcgatcgga aagcgccccg tgagcagcag cccgcggtac gcctcgccca
     6481 gggcgtgctg cagggcccgg tcgcggatga accggccgtt gagcagcaga tattgcgtgc
     6541 ggttgttcgc ccggctgtgc tgcggatcgg ccacgaagcc cagcagccgc accccttcgt
     6601 cgtggttgtc gatcgcgatc agcgagcttt ccagatcgcg gccgaagaac gcggcaatcc
     6661 gctcccgcca gtgctccgtg ggcggcaaat cgaacagcag cttgtcgttg tgccgcagcg
     6721 tgaaatgaat ctgcggctgg gcaagggcga tccgcgtgaa ggcctcggtg cagtgggcga
     6781 tttcggtctg cgaggcccgg aggaacttgc ggcggacggg cgtgttgtag aacaggttgc
     6841 ggacctcgat cgccgttccc gccggacagc cgcaggggac gatttccgac cgctgcccgc
     6901 cgacgacttc gagctcatac ccggcctcgg ccgcctgcag ccggctgcgg atcgtgaact
     6961 ggctgacctc ggcgatcgac gccagcgctt cgccgcgaaa gccgaacgtg gcgacgtgga
     7021 acaggtcgtc ggcatcggcg atcttgctcg tcgcgtggct cgaaacggct aagagcaggt
     7081 catcccgcgc gattccacag ccgtcatcga cgatgcgaat caggtcggtc ccgccctgac
     7141 cgagcgccac gtcgatccgc gtcgcccccg catcgacggc attctccaag agctctttga
     7201 cgacgctcgc cggccgctcg atcacttcgc ccgcggcgat cttgttgaca acgctcggcg
     7261 gcaattggcg aatggcgggc atgggcacat ccgtgtggcg agcaagcagc gagactattc
     7321 gcctgcgggc gggaggagtt cctgcaaatc cacaaccaat cgcgccgtgg cggagagatt
     7381 cacagcagct tcaccgggag cggtgagggt aacgtgactc tcgtattttc ctagcgccct
     7441 gttcggctgc ttgaacactt ccaaccgcgc ggcattcaga tcgacgatcc aatactcggc
     7501 aacgccggct cgcgcgtaga tgcggcactt gcgccaatcc tgctcgagcg acgactcggc
     7561 gacttcgatg accagcgcga cttcctcgcc cgtcgggtgc cggcggcgaa acttcgttgg
     7621 gtcgccaatt gcgataacca catctggttc gggctcgctg tcctctgtcc gcagaacatt
     7681 ctgaattctc gggaaccagc ccggcggcaa ctgccgtgac aatactgtat tgagatagtc
     7741 aatggtcgag tcgtgaagcg gattcttcgt cattttcttg acgatcagtc cctccaggag
     7801 ttcgaccgaa tcatcctcgg t
//
LOCUS       DASUGB010000695         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_355502, whole genome shotgun sequence.
ACCESSION   DASUGB010000695 DASUGB010000000
VERSION     DASUGB010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355502"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFV87_13315"
     CDS             complement(<1..926)
                     /locus_tag="VFV87_13315"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5104792.1"
                     /translation="MCNALLLLQGCDHSAAAPAAGAEAGTAAVDRVTAGPPQRKDLRL
                     TTTQPARIVAFEETALVSRISGYVAEVLVDIGDPVKKDQPLLRLSVPEMQDDVNQKLA
                     LLAQAEAEVGQAAADIEATKAAAESARAQVAKVEAGITRAGAECEYCQTEYNRVEALG
                     RSVTGEIVSAALNKLRAAQAAQQEAQAAVDAAQAAQRESEAGIRNAEADHDAAKARHK
                     VAAANLAYAQTMLAYAEIKAPYDGVVTQRNVDTGHYLQPSAVGQRPLLVVARTDQVRI
                     FVDVPELLAGYVNSGEQGDPATVNVQALPGRE"
     gene            complement(1073..>2654)
                     /locus_tag="VFV87_13320"
     CDS             complement(1073..>2654)
                     /locus_tag="VFV87_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5104793.1"
                     /translation="DAGQFRLRLRAPDGSHINFSEQVAKDALALIADEVGPDNVELTL
                     GYVGAIHSSYPINGVYQWSRGPEEAILLVDINDEIGLSIEALKERLRDRFAEKLPGVR
                     FSFEPADIVNEVMSFGSPTPVEVSVNGPNFAESRAFAQKVYQELAQVDALRDLQYGQS
                     LDYPTIEVKVDRERAGLAGLTPADVSRSLVTATSSSRFVVPNFWADPKSGIAYQVQVE
                     IPRPVVRAIDGVQMVGSTEDLGRIPLKRTAEGQVLVRDVANLAPGTMPGQYDRYNMRR
                     QVTMTANIADADLGTVGQQVAAALKRAGEPPAGMKVEVRGQIPPMQEMQNGLTIGLGL
                     AILVVFLLLAANYQSIRLSLATVSTAPAVVLGVVLMLLVTRTTLNIQSFIGAIMAIGV
                     AMANAILLVTFAEQRRRETDPDAPGVRVTAQAAAVTGAGGRLRAILMTSCAMIAGMLP
                     MALGLGEAGQQNAPLGRAVIGGLIAATAATLFVLPCVFTMIQARVKTASVSLDPGDPA
                     STYYSEGVGATTGTRGHA"
BASE COUNT          414 a          862 c          886 g          492 t
ORIGIN      
        1 atctcgcggc cggggagcgc ctggacgttg accgtcgccg gatccccctg ctcaccactg
       61 ttgacgtagc cagccagcaa ttccggcacg tcgacaaaga tgcgcacctg atcggtgcgg
      121 gcgacgacga gcagcggccg ctggccgacg gccgacggct ggaggtagtg gccggtgtcg
      181 acgttgcgct gcgtgacgac gccgtcgtag ggggccttga tctcggcgta ggcgagcatc
      241 gtctgcgcgt aggcgaggtt cgccgcggcc accttgtggc gtgccttggc tgcgtcgtga
      301 tccgcctccg cgttgcggat tccggcctcg ctttcgcgct gcgccgcctg agccgcgtcg
      361 accgccgcct gcgcctcctg ctgcgcggcc tgggcggcac gaagtttgtt cagggccgcc
      421 gacacgattt ccccggtcac cgagcggccc agcgcctcga cccggttgta ttcggtctgg
      481 caatactcgc attcggcgcc ggcgcgggta atgccggctt cgactttggc cacctgtgca
      541 cgggccgact cggcggcggc cttcgttgct tcgatatcgg ccgcggcctg tccgacttcc
      601 gcctccgcct gcgccaggag ggcaagtttc tggttgacgt catcctgcat ctcgggcaca
      661 gagaggcgca gcaggggctg gtctttcttc acggggtcgc cgatgtcgac cagcacctcg
      721 gccacgtagc cgctgatgcg ggagacgagg gcggtctctt cgaaggcgac gatccgcgcc
      781 ggctgcgtcg tggtgagccg caggtctttt cgttgcggcg gtccggcggt gacgcgatcg
      841 accgccgcag ttccggcttc agcaccggcg gccggcgcgg cagcggaatg gtcgcaaccc
      901 tggagaagga gaagtgcatt gcacattgca aattgcaaaa tgcaaattgc aaaatggtca
      961 atgccgctat cgtgaatctt tgcggaagca ggcgggcggg ccaatttgca atttgcaatt
     1021 actttcaatt gcggggaaat tctcgttccg cgcattagcc gtcgatcaag agtcatgcgt
     1081 gtccccttgt ccccgttgtt gcccctactc cctctgagta gtacgtgctg gccgggtctc
     1141 ccggatcgag cgacacggac gccgtcttga cccgcgcctg aatcatcgtg aacacgcacg
     1201 gtaagacgaa cagggtggcc gcagtcgccg cgatgagccc gccgatcacg gcccgcccca
     1261 agggcgcatt ctgctggccg gcttctccca gaccgagcgc catcggcagc atgccggcga
     1321 tcatggcgca actggtcatc aggatcgcgc ggaggcggcc gccggccccg gtcacggcgg
     1381 cggcttgagc agtgacccgg acgcctggag cgtccgggtc tgtttcgcgg cgccgctgct
     1441 cggcaaacgt caccagcaga atggcgttgg ccattgccac gccgatcgcc atgatggccc
     1501 cgatgaacga ctggatgttg agcgtggtgc gggtcacgag cagcatcagc acgaccccca
     1561 gcacgacggc cggcgcggtc gagaccgtcg ccagcgacag gcggatcgac tggtagttgg
     1621 cggccagtag cagaaatacg acgagaatgg cgagtccgag accgatcgtc agcccgttct
     1681 gcatctcctg catcgggggg atctggccgc ggacctcgac cttcatgccg gccggcggtt
     1741 cgccggctcg cttcagtgct gcggcaacct gctggccgac ggtccccagg tcggcgtcgg
     1801 cgatgttggc cgtcatcgtc acctgccgcc gcatgttgta gcggtcgtac tggccgggca
     1861 tcgtgcccgg ggccaggttg gcgacgtcgc ggacgagcac ctgtccctct gcggtgcgct
     1921 tgagtggaat gcgccccagg tcctcggttg agccgaccat ctgcacgccg tcgatcgcgc
     1981 gcaccaccgg gcgcgggatt tcgacctgca cctggtaggc aatgcccgat tttggatcgg
     2041 cccagaagtt gggcacgacg aaccggctcg acgaggtggc ggtcacgagc gagcgggaga
     2101 cgtcggccgg cgtcaatccg gcgagaccgg cccgctcgcg gtcgactttg acttcgatgg
     2161 tgggataatc gagcgactga ccgtactgca ggtcgcggag cgcgtcgacc tgcgccagtt
     2221 cctggtacac cttctgtgcg aatgcacggc tttcggcaaa attggggcca ttgaccgaga
     2281 cttccaccgg cgtcggcgaa ccgaaactca tcacctcgtt gacaatgtcg gccggttcga
     2341 acgagaaccg cacgccgggc agcttttcgg cgaacctgtc acgcagcctt tctttcaatg
     2401 cttcgatact gagcccgatc tcgtcgttga tatcgacgag caggatcgcc tcttccggtc
     2461 cgcgcgacca ctggtagacg ccgttgatgg ggtagctgga atgaatcgcg ccgacgtagc
     2521 cgagcgtcag ttcgacgttg tcgggtccga cctcgtcggc gatgagcgcc agcgcgtctt
     2581 tcgcaacctg ctcgctgaag ttgatgtgcg acccgtccgg cgcccgcagc ctgaggcgga
     2641 actgtccggc gtcc
//
LOCUS       DASUGB010000696         4324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1327707, whole genome shotgun sequence.
ACCESSION   DASUGB010000696 DASUGB010000000
VERSION     DASUGB010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327707"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1220
                     /locus_tag="VFV87_13325"
     CDS             45..1220
                     /locus_tag="VFV87_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5104794.1"
                     /translation="MAEPTKTSRRAFLTGKSATEALADAVLGPPPQLPPPQGLRPAER
                     QLLSVSREAMACLFEVVFDAARYRGLTDAAIEALELVSRLEDQLTIYRETSEVARLNR
                     RAADEAVAVEKGLFRLLQRAVALHDQTGGAFDITAGPLAKTWGFYRRQGQMPAAGEIA
                     AALAPVGSRHLQLDEPAHSVRFLRPGMELNLGAIGKGYALDRAADALVAAGISDFLLH
                     GGNSSVIARGNRILDFGLPILDSKNKGDQPLPVADQSKIQNPQSKIHRAWSVALRHPL
                     RPDVRLAEFDLCDQALGTSGSGTQFFHHQGKRFGHILDPRTGWPADKVLSVTVIAPTA
                     EQADALSTALYVMGLEEASEFCTSYAEISALLTTQSGVGQIDLHPLNLPADRWRILG"
     gene            1320..2306
                     /locus_tag="VFV87_13330"
     CDS             1320..2306
                     /locus_tag="VFV87_13330"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5104795.1"
                     /translation="MATVLTPGVKPPRGPETPCAKAESSQSLVVPEGASPLRVYWQFA
                     SIIVVVHLLSLLALVPWFFSWTGLFLCIAGHYVFGMLGITLGYHRLLAHKGFQCPKWL
                     ERCLALLGVCCLQDSPARWVAVHRMHHQHSDETPDPHSPLVAFLWGHVGWVMFRNRET
                     STLDFFQRYSRDLLSDPFYMWLERGEKRLSIYIAHAALFFLVGLAVGWISSGQYLAGL
                     QFGLSLLVWGVLARTVVVWHVTWSVNSLTHIWGYRNYNTSDNSRNNWLVGLLAHGEGW
                     HNNHHAQQRTAAHGHRWWEFDLTWHVIRALELVGLATNVVRPRNATEPTAEE"
     gene            complement(2314..3552)
                     /locus_tag="VFV87_13335"
     CDS             complement(2314..3552)
                     /locus_tag="VFV87_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5104796.1"
                     /translation="MEFRSHTLNNGLEVVAEISPSAYSAAYAFFVRTGARDETDAISG
                     VSHFLEHMVFKGSAKRSAADVNRDLDELSSSSNAFTSEEQTVYYSTTLPEDQERIVEL
                     LADMMRPVLRQEDFDTEKKVILEEIAKYDDQPPYGAHEKCMAAFFGPHPLGKSVLGTV
                     ESVGGLARDQMMAYFQERYSPRNMVLAAAGNVAYDDLIRQAEQHCGPWESFHAPRQHA
                     LPQPHRAWRVIQKPQAVQEYVIQIAAAPAAEDDERYAARLLATIVGDDSGSRLFWELV
                     DTGLAESASIGVQEFQRAGLFMTFLACSPDLAAENLHRLKDLLAQVEAEGVTEEELNQ
                     AKNKVCAHVVLSSERPTNRLFQVGNAWLQRRQYKTVREGVEAYRAVTLDDLAAVLKKY
                     PFTTSATVSTGPLAELKEPA"
     gene            complement(3554..>4324)
                     /locus_tag="VFV87_13340"
     CDS             complement(3554..>4324)
                     /locus_tag="VFV87_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5104797.1"
                     /translation="RNAQGTEETLATLTIGDVRRHWQERYSPQGAILSVAGKIDWPPL
                     KDRALSLFGNWKAGQPAAIAEEPPPRKYHHLPVESSQTHIGIALDAVPYTHPDYFQLR
                     GAVGVLSDGMSSRLFTEVREKRGLVYTVYAMMHSLKDRGAILAYAGTTAERAQETLDV
                     MLAELKLLFDGVQPAELDRLKGRIKRSLIIQQESSPSRAGSIALDWYYLSRVRTMAEL
                     SKIIDDLTPETINAYLAANRPRGFTVVTVGPEPLSFQT"
BASE COUNT          721 a         1386 c         1368 g          849 t
ORIGIN      
        1 tacttataat gtaacgagcc gaccgccgaa aaaggctcat ctgcatggcc gaaccgacga
       61 aaaccagccg ccgggccttc ctgaccggca aaagtgcgac tgaagcgctg gccgatgcgg
      121 tcctcggtcc gccgccccaa ctgccgccgc cgcaaggact tcgccccgcc gagcggcagc
      181 ttctttcggt cagccgcgag gccatggcct gcctgttcga ggtggtcttc gacgccgccc
      241 gctatcgcgg cctgacggat gccgcgatcg aggcgctgga gctggtttcg cggctggaag
      301 accagctcac gatctaccgc gagacaagcg aggtcgcccg gctgaaccgg cgggcggctg
      361 acgaggcggt ggccgtcgaa aagggcctgt tccgcctcct gcagcgggcc gtcgcgcttc
      421 acgatcagac gggcggggcg ttcgatatca cagccggacc gctggcgaaa acctggggct
      481 tctatcgtcg gcaggggcaa atgcccgccg ccggcgagat cgccgccgct ctcgcgccgg
      541 tcggcagccg gcatttgcag cttgacgagc cagcgcacag cgtccggttc ctgcgtccgg
      601 gcatggagct gaacctgggc gcgatcggca agggctatgc gctcgaccgg gcagcggatg
      661 cgctagttgc cgccggcatc agcgattttc tgctccacgg cggcaacagc agcgtcatcg
      721 cccgcgggaa caggattttg gattttggat tgccgatttt ggattcaaag aacaaaggcg
      781 atcagccgtt gcctgttgct gatcaatcca aaatccaaaa tccacaatcc aaaatccacc
      841 gcgcctggtc cgtcgccctc cgccatccgc tgcggcccga cgttcgcctg gccgagttcg
      901 acctgtgcga ccaggccctg ggcaccagcg gctctgggac gcagttcttc caccaccaag
      961 gcaagcgctt cggccacatt ctcgaccccc gcaccggctg gccggccgat aaggttcttt
     1021 cggtcaccgt catcgctccc actgccgagc aggccgatgc cctttcgacc gcgctgtatg
     1081 tcatggggct tgaggaggcc agcgagtttt gcacaagcta cgctgagatt tccgctttgc
     1141 tgacaaccca atcgggcgtt ggccaaattg acctgcatcc gctaaatttg ccagcggacc
     1201 gctggcgaat cttggggtga atagagttct cggttcttag ggcattaggc aaggcggcac
     1261 ggatgggcta caatcgccgg ccaactccgg cgatccctcg aaaggaatcg acttctcgca
     1321 tggccacagt cctgactcca ggcgtcaagc cgccccgcgg tccggagact ccctgcgcca
     1381 aagctgagag cagccagtcg ctggttgtgc ccgaaggcgc cagccccctg cgagtttatt
     1441 ggcagttcgc atcgatcatc gtcgtcgttc atttgctcag cttactggct ctcgtgccgt
     1501 ggtttttcag ttggacgggc ctgttcctct gcatcgcggg tcattacgtc tttggaatgc
     1561 tcggaatcac actcggctac catcggctgc tagcacacaa aggatttcag tgtcccaagt
     1621 ggctggagcg ttgcctggcg ttgcttggcg tctgctgttt gcaggattcg ccggcccgtt
     1681 gggtggccgt gcatcgcatg catcatcaac attccgacga aacgcccgat ccccacagtc
     1741 cactggtcgc cttcctctgg ggccacgtcg gctgggtaat gtttcggaac cgcgaaacca
     1801 gcacgctcga cttcttccag cgttattcgc gcgacctgct ttcggaccca ttctacatgt
     1861 ggctcgaacg gggcgaaaaa cgactatcga tctacatcgc ccatgccgcg ctgtttttcc
     1921 tcgttgggct ggctgtcggc tggatcagct cgggtcagta tttggccggt ttgcaatttg
     1981 gcttgagttt gctcgtgtgg ggggtcttgg cgcgaacggt tgtagtctgg cacgtcacct
     2041 ggtcggtcaa ttcgctaacc cacatctggg gttaccgcaa ctacaacacc agcgacaaca
     2101 gccgcaacaa ctggctggtg ggcctgctgg cccacggcga gggttggcac aacaaccatc
     2161 acgcccagca gcggaccgcg gcgcatgggc accgctggtg ggagttcgat ctgacctggc
     2221 acgtgattcg cgcgctggag ttggttggcc tggcgacgaa cgtcgtgcgg ccgcggaacg
     2281 cgaccgagcc gacggcggaa gagtagctcg caattacgct ggctctttca gctcggcgag
     2341 cggaccggtc gataccgtgg cgctcgtcgt gaagggatat ttcttcagga cggcggccag
     2401 gtcgtcgaga gtcactgcgc ggtaagcctc caccccttcg cggaccgtct tgtattgccg
     2461 ccgctggagc caggcgttgc caacctggaa cagccggttg gtcggccgct cggacgagag
     2521 cacgacgtgg gcgcagacct tgttcttcgc ctggttcagc tcctcctcgg tgacgccctc
     2581 cgcttccact tgggcgagca ggtctttcag ccggtggagg ttctcggcgg ccaaatcggg
     2641 cgagcaggcg aggaacgtca tgaacaaccc ggcccgctgg aactcttgca cgccgatcga
     2701 cgccgattcg gccagccccg tatcgaccag ctcccaaaac agccggctgc cgctgtcatc
     2761 gccgacaatc gtggccagga ggcgggcggc gtagcgctcg tcatcttccg ctgccggggc
     2821 ggcggcgatc tgaatgacat actcctgcac ggcttgcggc ttctgaatca cccgccaggc
     2881 ccgatgcggc tgcggcagcg catgctggcg gggggcgtga aacgattccc atgggccgca
     2941 atgctgctcg gcctggcgga tcaggtcgtc gtaagccacg ttgccggccg cggccaggac
     3001 catgttccgc gggctgtagc gctcctggaa ataggccatc atctgatcgc gcgccaggcc
     3061 gccgacgctc tccaccgtcc ccagcacgct cttgcccagc ggatgcggcc cgaagaacgc
     3121 ggccatgcac ttctcgtgcg ccccgtaggg cggctggtcg tcgtacttgg cgatctcctc
     3181 caggatcacc ttcttctcgg tgtcaaaatc ctcctgccgc agcaccggcc gcatcatgtc
     3241 ggccagcagc tcgacgatcc gctcctggtc ctcggggagc gtcgtggagt aatagacggt
     3301 ctgctcttca ctcgtgaagg cgttggagct ggagctcagc tcatccaggt cgcgattcac
     3361 gtcggccgcc gagcgcttgg ccgatccctt gaagaccatg tgctccagga aatggctgac
     3421 gccggaaatc gcgtcggtct cgtcccgcgc cccggtgcgg acgaagaacg catacgccgc
     3481 cgaataggcg ctgggactga tctcggccac aacttccagg ccgttattga gggtatggct
     3541 acggaattcc atgttacgtt tggaatgaaa gtggttccgg cccgaccgtc acgaccgtaa
     3601 accctcgcgg gcggttggcg gcgaggtagg cgttgatcgt ttcgggcgtc aggtcgtcga
     3661 taatcttgct cagctcggcc atcgtgcgaa cgcggctgag gtagtaccag tcgagggcga
     3721 tcgagccggc ccgcgagggg ctgctctcct gctggatgat cagcgagcgt ttgatgcgtc
     3781 ctttcaggcg gtcgagctct gcgggctgca cgccgtcgaa caggagcttc agctcggcca
     3841 gcatcacgtc gagcgtttcc tgggcccgct cggccgtcgt gccggcatag gccaggatcg
     3901 cgccgcggtc cttcaggctg tgcatcatcg catacacggt atagaccagc ccccgcttct
     3961 cgcgcacttc cgtgaacagc cgcgagctca tcccgtcgct gagcacgccg acggcgccgc
     4021 ggagctggaa atagtcggga tgcgtgtagg ggacagcatc gagggcgatg ccgatgtgcg
     4081 tctggctcga ttcgacaggc aggtggtggt attttcgcgg cggaggctcc tcggcgatgg
     4141 ccgccggctg gccggctttc cagttgccga agagcgacag ggcgcggtct ttgagcggag
     4201 gccagtcgat ctttccggcg acgctgagaa tcgctccttg gggcgaatag cgctcttgcc
     4261 agtggcgccg gacatcgccg atcgtcagtg tcgccagcgt ttcctcggtc ccctgggcat
     4321 tgcg
//
LOCUS       DASUGB010000697         4122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1050197, whole genome shotgun sequence.
ACCESSION   DASUGB010000697 DASUGB010000000
VERSION     DASUGB010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050197"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1371
                     /gene="mtaB"
                     /locus_tag="VFV87_13345"
     CDS             85..1371
                     /gene="mtaB"
                     /locus_tag="VFV87_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX5104798.1"
                     /translation="MPATLKTLTLGCKVNQYETELVREGFARIGYAEAAEGQPADLCV
                     VNTCTVTAEGDSKSRQAIRRLARENPQARIVVMGCYATRAPEELAKLPGVSEVLTDKR
                     EIPDLLGRFGVLDVPTGISQFGNRHRAYVKVQDGCLLRCSYCIIPHVRPHMHSRPLAH
                     IVDEVERLAGNGYREIVLTGVHLGHYGVEGNWNKPKDQWLRLSTLVRRLAELPGEFRL
                     RLSSIEATEVTRELIAVMAEYPERIAPHLHICLQSGSDAVLRRMKRRWGSRLFIDRCR
                     LVREALDNPAITTDIIVGFPGETDADFEATLRVSRECRFSKIHIFPFSPRKGTPAAEM
                     PDQVAPQVKSDRCERLAEVERELRTNYFRCLIGRELTVLVEGESNTAPGRWAGTSCRY
                     APAEIAADGLRAGSLVGVNAQRVSDCGTRIEAIGRG"
     gene            1576..>4122
                     /locus_tag="VFV87_13350"
     CDS             1576..>4122
                     /locus_tag="VFV87_13350"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5104799.1"
                     /translation="MGIVLPALADDENPAFECRWARGPIKIDGVADDADWEHAQTIDK
                     FSLPWLKEKARPAKTATKARLLWDRENLYFFAEMDDADLYADIKDHDGMTWYNDVFEL
                     FFKPADDKPGYYEFQVNAAGTVMDMFLPRRGAGGYARFIKDGEFHLEAKVKLRGTLNK
                     WTDEDQGWSVEGRIPWTDMLRTGGRPEIDETWSFALCRYDYSVAFEGPELSTCAPLGQ
                     SNFHAHEDYAPLKFLGPDSNPRKLGLQKQPLTTSKVVGSPDPPLPYRPVRAFPELKLT
                     YPIALDRVPGTDDLLVIAQGKSYGETTIYRVQDDPAVKEAVTLLETPSGGTAYGTCFH
                     PQFATNGYLYVGWNGRPPGDDKTPKRCYITRYTWDAAARKIDAGSRRDIIEWESDGHN
                     GADMEFGLDGMLYITSGDGTSDSDTNMRGQDLTQLTAKVLRIDVDHPEQGEPGGVSPR
                     RNYSVPKDNPFVGQEGIVPETWAYGLRNPWRITVDPKTGHVWVGNNGQDLWEQIFFVR
                     KGDNYGWSVYEGSHIFYAERKLGPHPHVLPAAEHHHSEARSLTGGVVYYGTKLPELVG
                     AYIYGDHSTGRIWAMKHDGEKVVWHKLLADTTFNISGFGLDSHGELLVADHRGEGNGA
                     YYYLEPTPPVTEPSMFPRKLSESGLFAEVKQHAMQPGVVPYSVNSPLWSDGAHKERFL
                     AIPHKEGEDMRIGFSTNRGWNFPDETVLIKSFALETTQGDPASRRWIETRFLTKQAGE
                     WVGYSYLWNDEQTDAELVATEGADREFEIRVPRSRENPDGLSRQKWRYPSRTECMVCH
                     SRAANFVLGLTELQMNKDHDYGHGHVENQLAVLERLGMLKVNAAADALKFL"
BASE COUNT          793 a         1271 c         1360 g          698 t
ORIGIN      
        1 actgctcgcc atcagggttt ttccaccaca ccacgtccct gaagaagttc gctacgaaat
       61 cgaaggggaa gtcgagtagc tcgaatgccc gccacgctta aaaccctcac cctcggctgc
      121 aaggtgaacc agtacgaaac ggagctggtg cgggaggggt ttgcgcggat cgggtatgcg
      181 gaagcggcgg agggacagcc ggcggatttg tgcgtcgtca atacttgcac ggtgacggcg
      241 gagggggaca gcaagagccg gcaggcgatc cggcggctgg cgcgggagaa tccgcaggcc
      301 cggatcgttg tgatgggctg ctatgcgact cgggccccgg aggaattggc gaagctgccg
      361 ggcgtgagcg aggtgctgac cgacaagcgg gagattcccg atctgctcgg ccgcttcggc
      421 gtgctcgacg tgccgacggg catctcgcag ttcggcaacc gccaccgggc gtatgtcaaa
      481 gtgcaggacg gctgcctgct gcggtgcagt tactgcatca ttccgcacgt ccgtccgcac
      541 atgcacagcc ggccgctggc gcacatcgta gacgaagtcg agcggctggc cggcaacggc
      601 tatcgcgaga ttgtgctgac gggcgtgcat ctgggacatt acggcgtcga aggcaactgg
      661 aacaagccca aggaccagtg gctgcggctg tcaacgctcg tccgcaggct ggccgaattg
      721 ccgggcgagt tccgcctgcg gctgtcgagc atcgaagcga ccgaggtcac gcgcgagctg
      781 atcgccgtga tggccgagta tcccgagcgg atcgccccgc acctgcacat ctgcctgcaa
      841 agcggcagcg acgccgtcct ccgccggatg aagcgccgct ggggcagccg gctgtttatt
      901 gaccgctgcc ggctggtgcg cgaggcgctc gacaatccgg cgatcacaac cgacatcatc
      961 gtcggctttc ccggcgaaac cgacgccgat ttcgaggcca cgctccgcgt cagccgcgag
     1021 tgccgcttct cgaagattca catcttcccc ttcagcccgc gaaaggggac gcccgccgca
     1081 gagatgccgg accaagtcgc gccgcaagtg aagagcgacc gctgcgagcg gctggccgag
     1141 gtcgaacgcg agctgcgcac taactatttc cgctgcctca tcggccgaga attgaccgtc
     1201 ctcgtcgaag gcgaaagcaa cacagcccca ggccgctggg ccggcacaag ctgccgatac
     1261 gcgcctgccg agatcgccgc cgacggcttg cgagccggga gtcttgtggg cgtaaatgca
     1321 caacgggtct cggattgcgg cacgcgtatc gaggcgattg gccgcggatg aacgcgggtt
     1381 ttcgcagatc ggcaatccgt gtttgatctg tgttccatcc gtggctcctt ccgcccgaca
     1441 gtttgcttgt atcgcccagt tcggccacgt agagtagcgg ttggtttcac atcccgcctg
     1501 gttcgcctcc cgcctggtcg ctctatgaat ggctctcggt tcgccgcctg gtttcttggt
     1561 gcaatcgttc tctgtgtggg aattgttctg ccggcgctgg cagacgacga gaatccggcg
     1621 tttgaatgtc gctgggcgcg ggggccgatc aagatcgacg gcgtggcgga cgacgccgac
     1681 tgggagcatg cccagacgat cgacaagttc tcgctgccgt ggctgaaaga gaaggcccgc
     1741 ccggcgaaga cggcaacgaa agcgcgcctc ttatgggacc gcgagaacct gtatttcttt
     1801 gccgagatgg acgatgcgga cctgtatgcc gacatcaagg accacgacgg catgacctgg
     1861 tacaacgacg tcttcgagct gttcttcaag ccggccgacg acaagccagg ctattacgaa
     1921 ttccaggtca acgccgccgg caccgtgatg gacatgttcc tgccgcggcg cggagccggc
     1981 ggctatgcgc ggttcatcaa ggacggcgag tttcacctcg aagcgaaagt gaagctccgc
     2041 gggacgctca acaagtggac cgacgaggac cagggctggt cggtcgaggg gcgaattccg
     2101 tggacagaca tgctccgcac cggcggccgg ccggagatcg acgaaacgtg gtcgttcgcc
     2161 ctctgccgct acgattattc cgtggcgttc gaaggaccag agctttcgac ctgcgcgccg
     2221 cttggccagt ccaatttcca cgcgcatgag gactacgccc cgctcaaatt cctcggcccg
     2281 gacagcaacc cgcgcaaact tggcctgcaa aagcagccat tgacgacgag caaggtcgtc
     2341 ggctcgcccg acccgccgtt gccgtaccgg ccggtgcggg cgtttccgga actgaagctg
     2401 acgtatccga tcgccctcga tcgcgtgccg ggtacggacg acttgctcgt catcgcccag
     2461 ggcaaatcgt acggcgagac gacgatctat cgcgtccagg acgatcccgc cgtaaaggag
     2521 gcggtgaccc tgctggaaac gcccagcggc gggacggcct acggcacctg ttttcatccg
     2581 cagtttgcga cgaatggcta tctgtacgtc ggctggaacg gccggccgcc gggcgatgac
     2641 aagacgccca agcgttgcta catcactcgc tatacgtggg acgctgccgc tcgcaagatc
     2701 gacgcgggat cgcgccgcga catcatcgag tgggagtccg acggtcacaa tggcgcggac
     2761 atggagtttg gcctggacgg catgctctac atcacgtcgg gcgacggcac aagcgattcg
     2821 gacacgaaca tgcgcgggca agacctgacg cagctcaccg ccaaagtgct gcggatcgac
     2881 gtcgatcatc ccgagcaagg cgagccgggc ggcgtcagcc cccggaggaa ttacagcgtg
     2941 ccgaaggaca atccgttcgt cggccaagaa ggcatcgtgc ccgaaacgtg ggcctatggg
     3001 ctgcggaacc cgtggcggat caccgtcgat cccaagacgg gccacgtctg ggtcggcaac
     3061 aacgggcagg acttgtggga gcagattttc tttgtccgca aaggcgacaa ctacggctgg
     3121 agcgtctatg aggggagcca catcttctat gccgagcgga agctcgggcc gcatccgcac
     3181 gtgctgccgg cggcggagca ccatcacagc gaggcccgct cgctgactgg cggcgtcgtg
     3241 tattacggca cgaagctgcc ggagctggtc ggggcctata tctatggcga tcactcgacc
     3301 ggccgtattt gggcgatgaa acacgacggc gagaaggtcg tctggcacaa actgctggcc
     3361 gacaccacgt tcaacatcag cgggttcgga ctcgattcgc atggcgaatt gctcgtggcc
     3421 gatcatcgcg gcgagggcaa cggggcgtat tactacctcg agccgacgcc gccggtcacc
     3481 gagccgagca tgttcccgcg gaagctgagc gagagcgggc tgtttgcgga ggtcaagcag
     3541 cacgccatgc agccgggggt cgttccctac tcggtcaatt cgcccctctg gtccgacggc
     3601 gcgcacaagg agcgattcct ggcgatcccg cacaaggaag gcgaggacat gcggatcggc
     3661 ttttcgacca atcgcggctg gaattttccg gatgagaccg tgctgatcaa gtcgttcgcg
     3721 ttggagacaa cccagggcga cccggccagc cgccgctgga tcgagacgcg gttcctcacc
     3781 aagcaagccg gcgaatgggt cggctattcg tacctgtgga acgacgagca gaccgatgcg
     3841 gaactggttg cgaccgaggg ggccgaccgc gagttcgaaa tccgcgtacc gcgctcgcgc
     3901 gaaaacccgg acggcctatc gcggcagaag tggcggtacc ctagccgcac ggagtgcatg
     3961 gtctgccaca gccgggcggc gaatttcgtc ctcggactga ccgaattgca gatgaacaag
     4021 gatcacgatt atggccacgg gcacgtcgag aaccagcttg ccgtgctgga gcgtttgggg
     4081 atgctgaagg tcaacgccgc agccgacgcg ctgaaattcc tg
//
LOCUS       DASUGB010000698         4737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1466228, whole genome shotgun sequence.
ACCESSION   DASUGB010000698 DASUGB010000000
VERSION     DASUGB010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466228"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFV87_13355"
     CDS             complement(<1..563)
                     /locus_tag="VFV87_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5104800.1"
                     /translation="MSARTIAIGDIHGCSEALRVVLSAIEPQETDTIVPLGDYIDRGP
                     DSRGAIEQLLTLEKQCRVVPLLGNHELMLIDALQNPYAVGPWRDCGGDATLASYGGSL
                     LKIPGSHLEFIRRCRRYYETATHFFIHANYQYDLPLDEQPDYLAYWEHIYVHLPLWAN
                     RSRISPQPHENGKIAVVGHTSQKNSEIL"
     gene            706..1821
                     /locus_tag="VFV87_13360"
     CDS             706..1821
                     /locus_tag="VFV87_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5104801.1"
                     /translation="MSELDFNAAASRGRQKRLLAEMQRLKLDLAIVQTIEHVQYLTGA
                     RFPWTFSPFAALRADGHLTLVAPKKPILESAADEVVFYEAQWHSTMRNDQRAASSELL
                     LKALAGKTAGIKRIGVEFSCFTSHLAALAGERVDIEPTLYQMRRIKDPDELARLKKAI
                     AGTGKMYALARQIIEPGIAEIEVFNRLQSAAVEEFGEQIAPATGNDYQCGARGGHPRN
                     GRQAQAGELYILDLGPAYRGYFSDNARTIAVNKQPTDEQHTAWTHIMGAFKHIESTAR
                     PGKSCRELFEEVQALLNEAPVGVFNHHLGHGIGLFPHEGPHLNPFWNDTFQVGEVFTC
                     EPGLYDEQHLRAGMRLENDYVVTETGVEILSPFPMEL"
     gene            1914..3977
                     /locus_tag="VFV87_13365"
     CDS             1914..3977
                     /locus_tag="VFV87_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5104802.1"
                     /translation="MSGLNPAQDEAVNTLRGPMLVLAGAGSGKTRVVTFRIANLIKHR
                     TRPERILAVTFTNKAAAEMQERISALLGKLKERPQISTFHAHCVRVLRRHANKLGYPQ
                     RFAIYDRGDQESLARGVLREIRVPSEMMRPSDLLYQISRWKTFSLRPADAAKAATSDK
                     EHLAAMGYRRYQKSLQNAGAVDFDDLLLLTEELFTQHAAARKQEAGLFDHLLVDEYQD
                     TNRSQYRIIKALAAEHRNLCVVGDDDQSIYGWRGAEVEHILRFAKDWPEAKVVRLEWN
                     YRSTAAILEIANRLIGFNKHRHDKVLRAARAGGEAPRIQQYNSETEEAEQTVADIRRR
                     IETDRLQPKDFAILFRTNEQPRAFETELRKAKIPYVILGSQSFFDRKEVKDVLAYLRT
                     IDSPRDEVSLLRIINNPPRGIGDKTVETLLKEAVTRGQPIWEVLRGVGVPPARLPANA
                     QESVAKFASLIDRYHQQLGHPHPSPLPKGEGAGGTATTGRGFGAGKSLVDLVRRLLAE
                     IGYEAELKRQYSDPNEQQARWQSVEEVVNALGIYEKSAKKPSLGGFIDEVALGQQDMG
                     YEKDKQLAKNAVALLTMHAAKGLEFPHVYLVGLEEGLLPHHRSLGADQDVDEERRLCY
                     VGVTRAQERLTLSLALTRMKWGKPRDTIPSRFLYEIMGQAEKAPSGARHKPRLAARPR
                     NTPSR"
     gene            4066..>4737
                     /locus_tag="VFV87_13370"
     CDS             4066..>4737
                     /locus_tag="VFV87_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5104803.1"
                     /translation="MWPAKQIDISWSDLAFAAATCFSPGRAAASARQLEERWAAEEGL
                     ACLSVRTGFDLLWAALDLPAGSEVLMSAVTIPDMVRIVQQHRLTPIPIDLDLETLAPR
                     QESIEEAVTPRTRAIVVAHLFGSRVPMGPILELARRHGLPVIEDLAQGFAGPNDRGHP
                     DSDMALFSFGTIKTATALGGALLRVRDRELLARMRAIAADYPLQPRRSYLQRVLKYAT
                     FKAGLI"
BASE COUNT          908 a         1515 c         1540 g          774 t
ORIGIN      
        1 aggatctcgc tgttcttctg ggaggtgtgg ccgacgaccg cgatttttcc gttctcgtgc
       61 ggctggggcg aaattcggct gcgattggcc cacagcggca gatgaacgta aatgtgctcc
      121 caataagcca gatagtccgg ctgctcgtcc agcggcagat cgtactgata gttggcgtga
      181 atgaaaaagt gcgtcgccgt ctcgtagtac cgccggcaac ggcggatgaa ctccaggtgc
      241 gacccgggaa ttttcagcag gctgccgccg tagctggcga gtgttgcgtc gccgccgcaa
      301 tcgcgccacg gaccgacggc gtatggattc tgcagcgcgt cgatcagcat cagctcgtga
      361 ttgcccaaca gcggcacgac gcggcattgc ttttcgagcg tcaggagctg ctcgatcgcg
      421 ccgcggctgt ccgggccgcg gtcgatgtag tcgcccagcg ggacgatcgt gtcggtttcc
      481 tgcggctcta tcgcggaaag caccacgcgc aacgcctccg agcagccgtg aatgtcgccg
      541 attgcgatcg tgcgtgccga catcggtcca cccgctggcg agccggaata aagcgcgata
      601 gtttagcagc caagtcgtgg ggcaggcatc ttgcctgccg aaatgcgcgt tgcgttttcc
      661 gaattcaaag tccttcttgg caggctggaa gcctgcccca cgaacatgag cgaactcgat
      721 ttcaacgccg ccgcctcccg cggccggcaa aagcggctgc tggccgaaat gcagcggctg
      781 aagctcgacc tggcgatcgt gcagacgatc gagcacgtgc agtacctgac cggggcccgc
      841 tttccctgga cgttttcgcc ctttgcggcc ctgcgggccg atggccactt gacgctcgtc
      901 gctcccaaga agccgatcct cgaatcggcc gccgacgaag tcgtcttcta cgaagcccag
      961 tggcacagca cgatgcggaa cgaccagcgg gccgcgtcgt cggagctgct gctgaaggcg
     1021 ttggccggca agacggccgg catcaagcgg atcggcgtcg agttctcgtg cttcacatcg
     1081 cacctggccg cgctggccgg cgagcgggtc gatatcgagc caacgctgta tcaaatgcgg
     1141 cgcatcaagg accccgacga gctggcacgc ctcaagaagg cgatcgccgg cacgggcaaa
     1201 atgtacgccc tggctcggca gatcatcgag ccgggcattg cagagatcga ggtcttcaac
     1261 cggctgcagt cggcggcggt cgaggaattc ggcgagcaga tcgcccccgc gaccggcaac
     1321 gactaccaat gcggcgcccg cggcggccac ccgcgcaacg gccgccaggc ccaggccggc
     1381 gagctgtaca tcctcgatct cggccccgcc tatcgcggtt atttctccga caacgcccgc
     1441 accattgccg tcaacaaaca gccgaccgac gagcagcaca cggcttggac gcacatcatg
     1501 ggggcgttta agcacattga atcgaccgcc aggcccggca agagctgccg cgagctgttc
     1561 gaggaggtgc aggcgctgct gaacgaggcc ccggtcggcg tgttcaacca ccacctcggc
     1621 cacggcatcg gcctgttccc gcacgaaggg ccgcacctca acccgttctg gaacgacacg
     1681 ttccaagtcg gcgaggtctt cacctgcgaa cccggcctct acgacgaaca gcacctgcga
     1741 gccggcatgc ggctggagaa cgactacgtg gtgacggaaa ccggagttga gatcctcagt
     1801 ccgttcccca tggagctgta gaggaaggag acgggacaga ggacagagga cagaggacag
     1861 gaatccgccg aatccctgtc ctctgtcctc tgtcttctgt cctctagccc acgatgtccg
     1921 gtctcaatcc agcccaggac gaagcggtga atacgctgcg cggaccgatg ctggtcctcg
     1981 ccggcgcggg cagcggcaag acgcgggtcg tgacgtttcg gatcgcgaat ttgatcaagc
     2041 accggactcg acccgagcgg attctggccg tcacgttcac caacaaagcc gcggcggaga
     2101 tgcaggagcg gatttcggcc cttctgggca agctgaaaga gcggccgcag atcagcacgt
     2161 ttcacgccca ctgcgtccgc gtgctgcggc ggcacgcaaa taagctcggc tacccgcagc
     2221 ggttcgcgat ctacgaccgc ggcgaccagg agagtctcgc caggggcgtg ctgcgtgaga
     2281 tccgcgtgcc gagcgaaatg atgcgcccca gcgacctgct ctatcaaatc agccgctgga
     2341 agacattttc gttgcggccg gccgatgcgg cgaaagcggc gaccagcgac aaggagcacc
     2401 tggcggcgat gggctaccgc cgctaccaga agtcgctgca aaacgccggg gcggtcgatt
     2461 tcgacgacct gttgctcttg acggaggagc tgttcacgca gcacgccgcg gcgcgcaagc
     2521 aggaagcggg cctgttcgat catctgctcg tcgatgagta ccaggacacc aaccgcagcc
     2581 agtaccggat catcaaggcg ctcgccgcgg agcatcgcaa cctgtgcgtc gtgggcgatg
     2641 acgaccagtc gatctatggc tggcgggggg cggaggtcga gcacatcctg cggttcgcca
     2701 aggattggcc cgaggccaag gtcgtgcggc tggagtggaa ctatcgctcg acggcggcga
     2761 tcctggaaat cgccaaccgg ctgatcggct tcaacaagca ccggcacgac aaggtgctgc
     2821 gggccgcccg cgccggcggc gaagcgccgc gaattcagca gtacaacagc gaaacggaag
     2881 aagctgagca gaccgtggcc gacatccgcc ggcggatcga aaccgaccgc ctgcagccca
     2941 aggatttcgc gatcctgttc cgcaccaacg agcagccgcg ggcgttcgag acggaattgc
     3001 ggaaggcaaa aatcccgtac gtgatcctcg gcagccagtc gttctttgac cgcaaggaag
     3061 tcaaggacgt gctggcgtac ctgcggacga tcgactcgcc gcgcgacgaa gtctcgctgc
     3121 tccggatcat caacaacccg ccccgcggca tcggcgataa gacggtcgag acgctcctga
     3181 aggaagcagt cacccgcggc cagccgatct gggaggtcct ccgtggggta ggcgtcccgc
     3241 ctgcccgtct tcctgccaac gcgcaggagt cggtcgccaa attcgcctcc ctcatcgacc
     3301 gctatcacca gcaactcggg caccctcacc ccagccctct cccaaaggga gagggagccg
     3361 ggggaaccgc gaccactggt cgcggctttg gagccggaaa gtccctcgtc gatctggtgc
     3421 gccgcttgct ggccgaaatc ggttacgaag ccgagctcaa acggcaatac agcgacccca
     3481 acgagcagca ggcccgttgg caatcggtcg aggaagtcgt caacgccctc ggcatttacg
     3541 aaaaatcggc caagaagcct tcgctcggcg gcttcatcga tgaagtcgcc cttggccagc
     3601 aggacatggg ctatgaaaaa gacaagcagc tcgccaagaa cgccgtcgcg ctcttgacga
     3661 tgcacgccgc caaggggctg gagttcccgc acgtttatct cgtcggcctg gaggagggcc
     3721 tgctgccgca ccatcgcagc ctgggggccg atcaagacgt cgatgaagag cggcggctgt
     3781 gctatgtcgg cgtcacgcgg gcccaggaac gcctgaccct ctcgctcgcc ctcacccgca
     3841 tgaaatgggg caaaccccgc gacaccatcc ccagccgctt cctctacgaa atcatgggcc
     3901 aggccgaaaa ggcgcccagt ggggcgcgac acaaaccgcg gcttgctgcc cgaccgcgca
     3961 acacgccttc gcgctaatgc tggacatcgc tgtcgagggc gttggatgcg aaaccaggcc
     4021 caaacacgcc gggtaggcat ttttggagac ttgcggagcg gcaacatgtg gccggcaaag
     4081 cagatcgata tcagctggtc ggatctggcg tttgccgcag cgacgtgctt ttcacctggg
     4141 cgagcggcgg caagtgcgcg ccagctggag gagcggtggg ccgccgagga ggggctggcg
     4201 tgtctgtctg tgcggaccgg ctttgacctg ctgtgggccg cgctcgacct gccggcgggg
     4261 agcgaagtgc tgatgtcggc ggtgacgatc ccggacatgg tgcggatcgt ccagcaacat
     4321 cgcttgacgc cgattccgat cgatctggat ctggagacgc tcgccccgcg gcaggaatcg
     4381 atcgaggagg cggtcacgcc ccgcacgcgg gcgatcgtgg tggcccacct gttcggatcg
     4441 cgcgtgccga tggggccgat tctcgagttg gctcggcggc acgggctgcc ggtgatcgag
     4501 gatctggccc aaggctttgc ggggcccaac gaccgtgggc atccggattc ggatatggcc
     4561 ctcttcagct tcgggacaat caagacggcg acggcgctgg gcggggcatt gcttcgcgtg
     4621 cgcgatcggg agctgctcgc acggatgcgg gcgatcgctg ccgattatcc cctgcagccg
     4681 cgacggtcgt atttgcaacg cgtcttgaag tacgcgacgt tcaaagcggg actgatc
//
LOCUS       DASUGB010000699         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_77102,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000699 DASUGB010000000
VERSION     DASUGB010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77102"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFV87_13375"
     CDS             complement(<1..1055)
                     /locus_tag="VFV87_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104804.1"
                     /translation="MGFAERRELYQKLEEARGGRPLIVYVTSTRPGVDSRILPDAVPQ
                     LLDQLDAIERPDEKDGVDLLVVSHGGDPMTAWRAMTLLHERVKHVAVLVPQVAYSAAT
                     MLAMGANDIVMHPNGNLGPVDPQIEIHPKPGEPPQRFGFEDLAGFLQFIKTEVGLTDQ
                     EQIQRMFAMFCNQVGPVPVAISARASLLTATMGEKLLMLHMGDREKAKRIAESLNKKF
                     FNHGYPVSRREAKDEIGLQIKDVSKDVEKLMWEIWLDVEKELEVRKRFNPIFQVADNA
                     EAASALFGPVLQGNAPVLPMQLMNDVQIAPGGGGGGGGGGNAASTARALLDAFTSKVK
                     VVDVKLTTAIMESTRKG"
     gene            1383..2627
                     /locus_tag="VFV87_13380"
     CDS             1383..2627
                     /locus_tag="VFV87_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5104805.1"
                     /translation="MCDSGGTVPYNLVSPIALILNLLMWIPISIALYAGFGVLILGSI
                     VPPLGDGCAWICDGCLALTESCISLGRDLPGNHCWLPAPPWWWIGGFYAVIGVLAVFP
                     ALRPRWRWCLALGSTWLAGSLFLSNPSIPHSLQDEPRPLACNFVAVGHGIGIVLELPD
                     GKTILYDAGKLGSPLGAARPVSAVLWSRGVTHLDALIISHADADHFNGVPELLDRFSV
                     GAIYVSPVMFEGRQPAVAELREAIDRSQVPLLEIHAGQRLRTSPDVRIEVLHPPRRGV
                     IGSDNANSIVLLVEYAGRRILLTGDLESPGLDDLLAEEPLDCDCILAPHHGSRRSDPA
                     GFADWSTPELVIISGGRNVEDIPAIERVKNSFRLRGAEVFHTAEDGCVRVDIDAAGTV
                     SATTFRTHVRATDAMSAALGVE"
     gene            complement(2637..3110)
                     /locus_tag="VFV87_13385"
     CDS             complement(2637..3110)
                     /locus_tag="VFV87_13385"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEX5104806.1"
                     /translation="MRRYIAVLEDNDDRIAAMERCLADKFPFFERKFFRTASDTIDWL
                     GENMGVVICLCLDHDLEPPADRPTCEPGTGRDVAEFLAGVEPCFPVVLQTTNAFAGIA
                     MEAMLGEQGWRVERVMPYEDLVWVAEAWLPTVRQVIVDAAEIRRELLESPARREV"
     gene            complement(3146..>3544)
                     /locus_tag="VFV87_13390"
     CDS             complement(3146..>3544)
                     /locus_tag="VFV87_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104807.1"
                     /translation="RRAVSIDPASLKPPLDPYPYSQDARSRPADATLADGLYAYVRDD
                     HGTIWVLPDAPHVHPRILGGGRSALYAGDFRIDKGMIEDVTNLSGTFQFDDPAGLLAA
                     ADQLIQQGLAVLPGGVRFFPPDGSPPQVLR"
BASE COUNT          636 a         1160 c         1049 g          699 t
ORIGIN      
        1 ctgccctttc gcgtgctttc cattatagct gtcgtcaatt tgacatcaac aactttgacc
       61 ttggaggtaa atgcgtcgag caacgcccgc gccgtgcttg cggcgttgcc gccgccgccg
      121 ccgccgccgc cgccgccggg cgctatctga acgtcattca ttaactgcat cggcaggact
      181 ggcgcattcc cttgcaatac cgggccgaat agagcactgg cggcctcagc gttgtcggca
      241 acctggaaga tcggattgaa acgcttccgt acctcaagtt ccttctccac gtcaagccag
      301 atttcccaca tgagcttctc cacgtccttg ctcacgtcct tgatttggag cccgatttcg
      361 tcctttgcct ctctgcgact gacagggtag ccgtgattga agaacttctt gttaagggac
      421 tccgcgatcc ttttggcctt ctcccggtcc cccatgtgaa gcatgagcag tttttcgccc
      481 atcgtcgccg tgagcagcga cgctctggcc gatatcgcga caggcaccgg gccgacttgg
      541 ttacaaaaca tcgcgaacat gcgctgaatc tgttcctgat ccgtaagccc aacctccgtt
      601 ttgatgaatt gcaagaagcc cgccaaatct tcaaagccaa agcgctgcgg aggctcgccg
      661 ggtttgggat ggatttcgat ttgcgggtcc actgggccta gattgccgtt ggggtgcatg
      721 acgatatcgt tggcccccat cgccagcatc gtcgcggcgc tgtaagccac ctgtgggacc
      781 agtacagcca catgctttac gcgctcgtgc agaagtgtca ttgcacgcca ggcagtcatt
      841 gggtcgccgc cgtggctgac aaccaacaag tcaaccccat ccttctcgtc gggcctctcg
      901 atggcgtcta gttggtcaag aagctgcgga acggcatcgg gcagaatgcg gctatctacg
      961 ccgggtcgcg ttgacgtgac gtacacaatt agaggccgac caccacgcgc ttcctctagc
     1021 ttctggtaca gctcacgacg ttcggcaaac cccatttgtc tccccctcca aagcagtgac
     1081 aatagagtac cggaatcggg tgatgccgca aggcgcagga caataaaaaa acacagcaga
     1141 acctgccacc aagaaacgcc ccaggccgcc cttgcggacg gcccgttgcg ctaggcccca
     1201 ctacaggtgg tggcgggtgt attgtggcct gccggcgctg tagaatccag tcccgccagc
     1261 gggagtgtcc acctcccacc aaaatcgaac cacgccggca ggaaacgtgt ctccaggcgc
     1321 aacgtgtcca gctcgctaaa atgcgggcca gaaagggctg cgggacaccg ggataggatg
     1381 ggctgtgtga ctctggcggg acagtaccat acaacctcgt ctctcccatc gcactcattt
     1441 tgaacttgct gatgtggata ccgatttcca ttgcgctcta cgccggcttt ggggtgttga
     1501 tcctgggttc gatcgtcccg ccgctgggcg acggctgtgc ctggatttgc gacggctgcc
     1561 tggcgctcac ggaaagctgc atttcgctcg gccgcgacct gcctggcaat cactgctggc
     1621 tcccggcgcc cccgtggtgg tggatcggcg ggttctatgc ggtcatcggc gtgctggccg
     1681 tttttcccgc gctgcgaccg cgctggcgct ggtgcctcgc gctcggatcg acctggctgg
     1741 ccggatcgct gtttctatcg aatccctcga tcccgcacag cctccaggac gagccgcgcc
     1801 cgctggcgtg caatttcgtc gcggtcggtc acggcatcgg catcgtgctc gaattgccgg
     1861 acggcaagac gatcctctac gacgccggca agctcggctc gccgctcggc gccgcgcggc
     1921 ccgtttctgc ggtgctctgg tcgcgcggcg tcacgcacct cgacgcactt atcatttcgc
     1981 atgccgacgc cgaccacttc aacggcgttc ccgaattgct cgaccgcttc tccgtcggcg
     2041 cgatctacgt ctcgcccgtg atgttcgagg gccgtcagcc ggcggtcgcc gagttgcgcg
     2101 aggccatcga ccgctcgcag gtgccgcttt tggagattca cgccggccag cgcctgcgga
     2161 cgtcgccgga cgtccggatt gaagtgctgc acccgccccg ccgcggcgtc attggcagcg
     2221 acaacgcgaa cagcatcgtg ctcctcgttg aatatgccgg ccggcggatt ctgctgaccg
     2281 gcgatctgga gtctcccggc ctggatgatc tgctggccga ggaaccgctc gattgcgact
     2341 gcattctggc cccgcaccac ggcagcagac gcagcgaccc ggccggcttc gccgattgga
     2401 gcacgccgga gctggtcatc atcagcggcg gccgcaacgt cgaggacatc ccggccatcg
     2461 agcgcgtcaa aaactcgttc cgcctccgcg gggccgaagt cttccacacc gccgaagacg
     2521 gctgcgtgcg ggtggatatc gacgctgccg gaaccgtctc cgccacgacg ttccgcacac
     2581 acgttcgcgc gacggacgca atgtccgctg ccctgggcgt cgaatagaaa tacgcctcac
     2641 acttcgcgtc gtgcgggcga ctccaagagt tctcgccgga tttcggcggc atcgacgatg
     2701 acttgcctta ccgtcggcag ccatgcctcg gccacccaaa cgaggtcctc gtagggcatg
     2761 acgcgctcga ctcgccagcc ttgctccccc agcatcgctt ccatggcaat tccggcaaat
     2821 gcattggtgg tctgcaaaac gaccgggaag cacggctcaa cgcccgcaag gaattcggcg
     2881 acatcccggc cggtacccgg ttcgcacgtc ggcctatccg ccggcggttc caggtcgtga
     2941 tcgagacaga ggcaaatgac aacgcccatg ttctcaccga gccagtcgat cgtgtcgctg
     3001 gccgtgcgaa agaacttccg ctcaaagaac gggaacttgt cggccagaca ccgctccatc
     3061 gcagcgatgc ggtcgtcgtt gtcttcgagc acggcgatgt agcggcgcat ggcttgccgt
     3121 ttggctgagt ggtggttcgg agcgatcaac gcaggacctg cggcggcgat ccgtccggtg
     3181 ggaagaaccg taccccgcca ggtagtacgg caagtccctg ctggatcaac tgatcggcag
     3241 ccgccaacag gcccgcggga tcatcgaact gaaacgtgcc ggacaagttg gtcacatcct
     3301 caatcatacc tttgtcgata cggaagtcgc cagcgtaaag cgcgcttcgc ccgccgccga
     3361 gaattctcgg atggacgtgc ggcgcatctg gcagcaccca gatcgtcccg tggtcatcgc
     3421 gaacgtaggc atacagtcca tccgccaatg ttgcgtcggc tggccggctg cgcgcgtcct
     3481 gggagtaagg gtacggatcg agcggaggct tcaggctagc gggatcaatc gacacggcac
     3541 gtct
//
LOCUS       DASUGB010000700         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1607007, whole genome shotgun sequence.
ACCESSION   DASUGB010000700 DASUGB010000000
VERSION     DASUGB010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607007"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFV87_13395"
     CDS             <1..219
                     /locus_tag="VFV87_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104808.1"
                     /translation="LRLSAVNAGVRFRGADTDPVPLAGRVVVGMAAGASSDGLVLAAS
                     DARGCVAAAPFSARVGGLSSRGCPVVSS"
     gene            505..834
                     /locus_tag="VFV87_13400"
     CDS             505..834
                     /locus_tag="VFV87_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104809.1"
                     /translation="MRQVVLIGLLAAAAVTLASCSGVILLVALVLSNTGRSGHTPTYD
                     TPIGSYSSYSRYDDFGAGTQPIYVAPANDWPDNPQWSGSISHGTRGATVQDSIYSVDG
                     KVLNLPY"
     gene            1019..3199
                     /locus_tag="VFV87_13405"
     CDS             1019..3199
                     /locus_tag="VFV87_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5104810.1"
                     /translation="MPHSQTLEQLLAHWDEAYHNGVELSAEELCASHSSPDLLLEVRE
                     QIEALKEVDGLLKTHAWTTERGRSDTDLPGVFPSTEQRYSIHSHLARGGLGTVFIAED
                     RELRREVALKFVLGRYTDDPNALASFRSEREITARMQHPGIVPVYGVGELANGRPFYV
                     MRYIKGRSLKESIAEFHQRAGERPPAERELAFRKLLSSFASACQTVAYAHNRCVINRD
                     LKPDHIILGRYGETLVVDWGLAMRVDPPQASADKDANTVLADPSTSNSSRGCAGTLMY
                     MSPEQAAGAAFLTPATDIYSLGATLYVVLAGALPFAGESSVHELRQKVIRGEFAPPRQ
                     VNPRCPKALDAICRKAMSSKPEDRYPTAEALAADVERYLADQPVSVLRESRSESALRW
                     MRRHRGAALMGLMGLISLTLLALVASLAQFRSAHVANEARKTSLHTSATFAAKMLGRE
                     VESRWLALEVAAEDPELIQLLRRWEEETDKSSGESPLHPRFDTWLAARREQLDPAHPA
                     FSWFINDRRGIQVGRHAQDGKTFHGESFAHRTYFHGGEHDLASEQLQFARPIDRPNLS
                     PVFENRSYEDNSEQQHKLTATCSVPIRDGGQIVGVLGTSIEITAFEALRLDPSQEHVA
                     LLIDSRPDWKDDRGLVLFHGDQAGKSHPAPNNQRPTIDASLLSRMLALQSGSVASDVL
                     TDFRDPDAGDAPMAMAGYAPVWVRPDRTTPRFSGWIVIVCETNH"
     gene            complement(3226..>3666)
                     /locus_tag="VFV87_13410"
     CDS             complement(3226..>3666)
                     /locus_tag="VFV87_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEX5104811.1"
                     /translation="YAIANLIGDQAKGHTKVFAILNSDVQVRPTTLMVSKVTVKSGKY
                     TNILMGTVQAAIAHAVLDSVRAGDIPKRRVNDLGIIVSVWLDPSILEAESVDYELLFK
                     TNREATAKAIHKAMRHEPTIDWLLDNQDQVPHYFHQLAVEGKLE"
BASE COUNT          677 a         1194 c         1118 g          677 t
ORIGIN      
        1 ctgcggctgt cggccgtgaa cgcgggcgtg agattccgcg gagctgacac ggacccggtt
       61 ccgctcgccg gtagagtcgt cgtggggatg gcggccggcg cgagcagcga cggcctggtg
      121 ctggcagctt ccgacgcacg cggctgcgtc gcagccgcac cattctctgc gcgcgtcggc
      181 ggactgagca gccgcggctg cccggtcgtt agctcttgac cactcagtgc agagctggcc
      241 cagagaactg cgacgatcga aaacgaactc caccagcgcg cagccatccg cgacctccct
      301 gctccccccg gctgccagaa aagaagccca atgcgggcgg gagtatagga acgcccgcga
      361 gcaagcagca agggcaaggc gactacgcat caccctttcc tgactgatga ctgctagcag
      421 ttcacttgtt gcaaacgggc ttgttctgcg ctgtgaaaac tggacccctg cttccatgct
      481 cttttgaaag gaaggatgat cgccatgcgc caggttgtct tgatcggctt actcgccgcg
      541 gcggcggtca cgctggcctc gtgcagtggt gtgatcctgc tggtcgcgct cgtgctttcc
      601 aacaccggcc gcagcggcca cacgcccacg tacgacaccc cgattggcag ctatagcagc
      661 tacagccgct acgatgattt cggcgccggt acccagccga tctatgtcgc acccgccaac
      721 gactggcccg acaatccgca gtggagcggc agcatcagcc acggcacgcg aggagccact
      781 gtccaagaca gcatctactc cgtcgatggc aaggttctca atttgccgta ctaagtgaca
      841 gccaatccgt ggactggtcc ctgaaatcgc tgccagccga atgctggggc gccattcccg
      901 gcaggaattc tcgctcccag atactcaccc cggacaactt ccgtcaaaat agtttgtcac
      961 acttgctgca tggctgcgct ctggcttggc agggactgcg cacggggagc ccaacgacat
     1021 gccccatagc caaacgctcg agcagcttct cgcccactgg gacgaagcct accacaacgg
     1081 cgtggagctt tcggccgagg agctctgcgc cagtcactcg tcgcccgatt tactgctgga
     1141 ggtccgcgag cagatcgagg ccctcaagga agtggatggg ctgctgaaga ctcatgcttg
     1201 gaccaccgag cgcggtcggt cagatacgga tttgccggga gttttcccca gcaccgagca
     1261 gcgttactcg attcacagcc atctggcacg gggcggactg ggaacggtgt ttatcgccga
     1321 ggaccgcgaa ctgcggcgcg aggtagcgct caagttcgtt ttgggacgat acaccgacga
     1381 tcccaatgcc ctggcgagct ttcgctcgga acgcgaaatc accgctcgca tgcagcatcc
     1441 cggcatcgtg ccggtctatg gcgttggcga gctcgccaac ggccggccgt tctatgtgat
     1501 gcgctacatc aaggggcgtt cgctcaagga gtcgatcgcc gaattccatc aacgagcagg
     1561 cgagcgtccg ccggccgaga gagaactggc atttcgcaag ctgctgagca gcttcgcctc
     1621 cgcttgccag acggtggcct acgcgcacaa ccgctgcgtg attaatcgcg acctcaagcc
     1681 cgaccacatc atcctcggcc gctatggcga gacgctggtc gtcgattggg gcctggccat
     1741 gcgggtggac ccgccgcaag cgagcgccga caaagacgcc aacaccgttc tggccgatcc
     1801 ttcgacgagc aactcctccc gcggctgtgc gggaacgctg atgtacatga gcccggaaca
     1861 ggcggccggc gccgcgtttc tcacgccggc aaccgacata tacagccttg gcgcgacgct
     1921 ctacgtcgta ctcgccggcg cgctgccctt cgccggtgaa agctccgtcc acgagctgcg
     1981 gcagaaggtg atccgcggcg agttcgcacc gccgcgccag gtcaatccgc gttgccccaa
     2041 ggctctcgac gccatttgcc gtaaggccat gtcgagcaag ccggaggacc gctacccgac
     2101 ggcggaagca ctggccgccg acgtcgagcg ctacctggcc gaccagccgg tctctgtgct
     2161 ccgcgaatcg cgcagcgaat ccgcactgcg ctggatgcga cgccatcgcg gggcagcgct
     2221 gatgggacta atgggcctga tttcgctgac tctgctggca ctggtcgctt cgctggccca
     2281 gttccgctcg gcacacgtcg ccaacgaagc gcgcaaaacg agcctgcata cgtcggcgac
     2341 gtttgccgcc aaaatgctgg gccgggaagt cgaaagccgc tggttggcgc tggaagttgc
     2401 cgcggaggat ccagaattga ttcagctgct ccggcgctgg gaagaggaaa ccgacaagtc
     2461 atccggcgaa agtccgctgc atccgcggtt tgacacgtgg ctcgccgccc gccgcgagca
     2521 attggaccct gcacatcctg ccttcagttg gttcatcaac gatcggcgcg ggatccaggt
     2581 cggacgacac gcccaggacg gcaaaacgtt ccatggcgag tcatttgcgc atcgcacgta
     2641 ctttcatggc ggcgagcatg acctcgcgag tgagcaactg cagttcgctc ggccgatcga
     2701 ccggcccaac ctctctcccg tcttcgagaa caggtcgtat gaagacaatt cggagcagca
     2761 gcacaagctg acggctacgt gctcagtacc gattcgcgac ggcgggcaaa ttgtcggcgt
     2821 tcttggcact tccatcgaga ttacggcgtt tgaagcgctg cgcttggacc cgtctcagga
     2881 gcatgtcgcg ctcctgatcg actcacgccc ggattggaag gacgaccgcg gtctggtcct
     2941 ctttcatggc gatcaagcgg gcaagtcgca tccggctcct aacaaccagc ggcccacgat
     3001 tgacgcctcc ctcctctcgc ggatgctggc tctgcaatcg ggcagcgtcg ccagcgacgt
     3061 cctgacagat ttccgggatc cggatgctgg agatgcgccg atggccatgg caggatacgc
     3121 accggtctgg gtgcgacccg accggacgac tccccgcttc agcggctgga tcgtcatcgt
     3181 ctgtgaaacg aaccactgac gcgcgccggg cctgacgaaa gtgagctatt ccagcttccc
     3241 ctccaccgcg agctggtgga aatagtgcgg aacctggtcc tggttgtcca ggagccagtc
     3301 gattgtgggc tcatggcgca tcgctttgtg gatggccttg gcggtcgctt cgcggttggt
     3361 cttgaatagc agttcgtaat cgacgctctc cgcctcaaga atgctcgggt cgagccagac
     3421 gctgacaatg atccccaggt cgttcaccct ccgtttgggg atatcgccgg cccgcacgct
     3481 gtcgagcacg gcgtgggcaa ttgcggcctg cacggtcccc atcagaatgt tcgtgtactt
     3541 gccgctcttg accgtgactt tgctgaccat cagcgtcgtc ggccgcacct gcacgtcgct
     3601 gttgaggatc gcaaagacct tcgtgtgccc cttcgcctgg tccccgatca ggttggcgat
     3661 cgcata
//
LOCUS       DASUGB010000701         6451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1189254, whole genome shotgun sequence.
ACCESSION   DASUGB010000701 DASUGB010000000
VERSION     DASUGB010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189254"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..629
                     /locus_tag="VFV87_13415"
     CDS             204..629
                     /locus_tag="VFV87_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104812.1"
                     /translation="MTDSFDLSATDQTTIQIAASGTLTAILDAAGNLKVTDVSADGVD
                     NDLSLIRSGQNLIVSDAHERFSIVPPGGVLSDGGRTLTLPLALVTGSIAVHLQSGTDA
                     LGIEFGDANSPSSTITETYVEGLTGFAVAPTLRTRKSST"
     gene            704..2170
                     /locus_tag="VFV87_13420"
     CDS             704..2170
                     /locus_tag="VFV87_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5104813.1"
                     /translation="MKGGDGDDTFLLDLNTADDLTNSVPGLTAVILMGDGGGDTFGQT
                     PDNAPHQGSGLPALPIPPAALPAPILTPLPFPGIQRGKIRPSLTTSIVVHGGGADDPS
                     NVASPVGNATAGNSYGVSFDLLNLDLTDRRPLSQSDDSLAVVATVSGAATFSDPDPGE
                     SFQSIQFASIEAVNVCDQGALTRTAMGDLYLRGTEAIDIVQLLPFSGIVYRARINAAF
                     YMEPVTGRMIVYGRGGNDTIQQSNVSLPLEAFGEAGDDFIMGSGSSDLLVGGPGSDRL
                     YGGEGNNELWGDNLGQADGPSGGNDQLTGGGGNETAYGGGGNDIIYLYGGNDYAFGGW
                     GNDTIDGGLGDDRLFGGEGDDYILGNSGNDLLAGNAGNDRLYGQAGHDVLIGGLGADQ
                     LIGDSGNDLLVDGTIGYDPAGAMGPGSDASTFAGDASDVALLALLADWLDNFLLDSHD
                     GLAITHDTDPDKLSGGLGFDTAAGLLPPDSGDWDFLLP"
     gene            2208..3356
                     /locus_tag="VFV87_13425"
     CDS             2208..3356
                     /locus_tag="VFV87_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104814.1"
                     /translation="MFASPASSAESAGPPLDPEVFDDEQAIGQSGRLAELLRLWNTLV
                     RGEIGSVQSFVAYDHQAAVQQRWHQWLVGTAAVTGSAAVLLAILQLASFARPELFGSL
                     IKFFEFGEYIAAVLALVAVVLGLVVAFHTRWQLLRMKTEQYRFVKFHYLLHAGGWVSN
                     SEHERSEHLLTLLAGIHALDSHDAHEWSLGVFSLVDDDPAVIASADASLAEDIVRYFR
                     EKRIGSQRTYFTGQAQKRRASERKTWMIPPVCFFLSIVCALAHFSIEIFSQPPDQEHT
                     DSAGSMEKQEQPQVHPPNALMLACLIGAATFPVVGAMVRTMRTAFEFGRNANRFEGVA
                     RILASVDQQLQATTTPAVKLELLREAEFVLQHENRSWMRLMIEAEWFG"
     gene            3508..3680
                     /locus_tag="VFV87_13430"
                     /pseudo
     CDS             3508..3680
                     /locus_tag="VFV87_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039819.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3773..5314
                     /locus_tag="VFV87_13435"
     CDS             3773..5314
                     /locus_tag="VFV87_13435"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5104815.1"
                     /translation="MAAEFDTIVQWLTTCGAYPHNPATVERIETHISCVYLAGDQVYK
                     LKKPVQYDFLDFSTCEKRKYACHEELRLNRRLAPQAYLDVVPITRSSDNSLQIGGGGE
                     PLDWLVHMWRLPTDQMLDSLHRRGELRREQIDRLAEVLIDFYHSLEPLPISADEYRAR
                     LESHVRGNLRELMAVSHHLPPRIVERTHAFQLQLLKLRPEMFDQRVAQGRIVEGHGDL
                     RPEHICLIEPPAIFDCIEFNAGFRQLDVADELAFLAAECDILGAEWVGLRLLEKYREL
                     SGDQPLEILLDFYKSYRACVRGKVAALRADQLSGKDREAAAAEALRHVQLADRCVSQW
                     ARPLVLIVGGLSGTGKSTLAGALAESLGAELLRTDVIRRETIAVEQPQNRYSAESRQR
                     VYDAMFDRASRLHAEGLSVVLDGTFGSRQSFVGAVQIAKHPRTVVFAVECECPPEVAR
                     ERISRRLARENDPSEARPDLPGRQQQNWEAWPADVAHVRIDTLQTIDEQVRIVQNELA
                     GRWTV"
     gene            complement(5366..>6451)
                     /locus_tag="VFV87_13440"
     CDS             complement(5366..>6451)
                     /locus_tag="VFV87_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104816.1"
                     /translation="EPNVPLHPDGQPLRDTTGGDWTLFAAPFPLVRVPSTAEAFRDLT
                     RYEAFTCLKPGTRLDDPQIDRDELKRARYAWRKNTPPIGPAEQARLTKKGLLKPHEGL
                     LQLRDAETSKPVAAHAGSVCWNAFRKRFVLIVCELYGTSLLGETWYAEADSPVGPWVF
                     ATKIVTHEKYSFYNPKQHPFFDQEGGRLIYFEGTYTHTFSGNEHKTPRYDYNQIMYRL
                     DLADERLVLPVPLYDAEKNGAAADFAWRDAEDARQAEFEKIAFFALDRPRQDVVPVYR
                     VNEGGTARLTTGPPPNQRDAPPLFWALADGEKDVPGTSPLSEYVSDSGKPPLYDVRRD
                     RQPFGYKRADRPLCRVWLSPYRSADGK"
BASE COUNT         1160 a         1991 c         2067 g         1233 t
ORIGIN      
        1 ccgtgagttc gaagagggaa agaagatcac gtggcgggac ggcatcaccg cgctctggac
       61 gctggtgaag ttccgcttcg tcgagtagtg cgacgcgtcg cgcgacctgc ggagccgacg
      121 aatggctgat cgcgacgccg acgataccgg ccgccgattt ggcaggtttc tcggcgggaa
      181 cagcagttct aattcgcttg cgagtgacgg acagcttcga cctgtcggcg acggatcaaa
      241 caacgattca gattgccgcg agcggtacgc tgacggccat tctggatgcg gcgggtaacc
      301 tgaaagtcac ggacgtgtcc gccgatggcg tcgataatga tctgtcgctt attcgcagcg
      361 gccagaacct gatcgtcagc gatgctcacg agcgattctc gattgtgcct cccggcggcg
      421 tcctttcgga cggcggccgg acgctcacgc tgccgctcgc tctcgtcacc ggcagcatcg
      481 cagtccattt acagagcggc acggatgccc tgggcatcga attcggcgac gccaattctc
      541 cttcttcgac gatcacggaa acgtacgtcg agggattgac gggttttgcg gtggctccga
      601 cgttacgtac acgcaaatcg agcacctaac gatcaacggc acgagcggcg acgacgtctt
      661 cgatatcctc ctgagccccg gcagcagtct gaactccgtc actgtgaagg gcggcgatgg
      721 cgacgacacg tttctgctcg acttgaacac agccgacgac ctgaccaatt ctgtgccagg
      781 gctcaccgcc gtcattctga tgggcgacgg cggcggtgac acgttcggcc aaacgccgga
      841 caacgcccca catcagggaa gcggtctgcc tgcgttgccg atcccgccag ccgcgttgcc
      901 ggcgccaatt ctcacaccgc tgccgtttcc cggcatccag cgcggcaaga ttcgccccag
      961 tctgacgacg agcattgtgg ttcatggagg cggggcagat gatccatcca acgtcgcttc
     1021 accagttggc aacgcgacgg cgggcaattc ctacggcgtc agcttcgact tgctgaatct
     1081 cgacctgacc gaccgtcgcc cgctgagcca atcggacgat tccctcgccg tggttgccac
     1141 ggtgagtggc gctgcgacgt tctccgatcc cgaccccggc gagtcattcc agtcaatcca
     1201 attcgcgagc atcgaagctg tcaacgtttg cgatcaaggc gcgctcactc gcacggcgat
     1261 gggcgatctc tatctccgcg gaaccgaggc gatcgacatc gttcagttgc tgcccttcag
     1321 cggcattgtc tatcgcgccc gcatcaatgc ggcgttctac atggagcccg tcacggggcg
     1381 gatgatcgtc tatggccgcg gcggcaacga taccatccag caatccaacg tcagcctccc
     1441 gctcgaagcc ttcggcgaag cgggtgacga ttttatcatg ggctcgggga gcagcgactt
     1501 gttggtcggg gggccaggat ccgaccggct gtatggcggc gaaggcaaca atgagctctg
     1561 gggcgacaat ctcggccagg cggatggccc gtcaggcggc aacgaccagc tcaccggcgg
     1621 cggaggaaac gaaaccgcct acggcggagg cggaaacgac atcatctatc tttacggcgg
     1681 caacgactac gccttcggcg gctggggaaa cgacacgatc gatggcggcc tgggcgacga
     1741 tcgcctcttt ggcggcgagg gggacgatta catcctcggc aattcaggga acgatttgct
     1801 cgcgggaaac gccggaaacg atcggctgta tgggcaggca ggccatgacg tgctgatcgg
     1861 cggcctgggc gccgatcaat taattggcga ttccggcaat gatttgctcg tggatggaac
     1921 catcggctac gacccggcag gcgcgatggg gcctggctcg gacgcgagca cgttcgccgg
     1981 cgacgcgagc gatgtggccc tgctcgccct tttggctgat tggctcgaca acttcctgct
     2041 ggacagccat gatggcctcg cgatcacaca cgacacagat cccgacaagc tcagcggcgg
     2101 actgggcttc gatacggctg ccggtcttct gccgccggac tcgggcgatt gggacttctt
     2161 actgccgtag cggtaaatcg cgccatcttt agccggagta cgatcccgtg ttcgccagcc
     2221 ccgcctcctc agccgaatca gccggcccgc cgctcgatcc ggaagtcttc gacgacgagc
     2281 aggcgatcgg gcaatccggc cgcctggcgg aattgctccg gctgtggaac acgctggtgc
     2341 ggggcgagat tggcagcgtg cagtcgttcg tcgcctacga ccaccaggcc gccgtccagc
     2401 agcgctggca ccagtggctg gtcggcacgg cggccgtgac gggctcggcc gcggtgctgt
     2461 tggccattct gcagctcgct tcgttcgccc ggcccgaact gttcggctcg ctcatcaagt
     2521 tcttcgagtt cggcgaatac attgccgccg tgctggcgct ggtcgcggtc gtgctgggcc
     2581 tggtcgtcgc cttccatacg cgttggcaat tgctgcggat gaagaccgag caatatcggt
     2641 tcgtcaagtt tcattacctg ctgcacgccg gaggctgggt cagcaattcc gaacatgagc
     2701 ggagcgagca cctgttaacg ttgctggcag gaattcatgc gctggacagc cacgacgccc
     2761 acgagtggtc gctaggtgtc ttttcgctgg tcgatgacga cccggcagtg atcgcctcag
     2821 ccgatgcgtc gctcgcggag gacatcgtcc gttacttccg cgagaagcgg atcggctcgc
     2881 agcggaccta cttcaccggg caggcgcaga agcgccgcgc gagcgagcgc aagacatgga
     2941 tgattccgcc cgtgtgcttc ttcctgagca ttgtctgcgc gctggcccac ttctcgatcg
     3001 agattttcag ccaaccgccg gaccaggagc acacggattc ggccggctcg atggaaaagc
     3061 aagagcagcc ccaggttcat ccgcccaacg ccctgatgct ggcgtgcttg atcggcgcgg
     3121 cgacatttcc ggtcgtgggc gcgatggtgc ggacgatgcg gactgccttc gaatttggcc
     3181 gcaatgccaa ccgctttgag ggcgtcgcgc ggatcctggc cagcgtcgat cagcagcttc
     3241 aagcgaccac gactcccgcg gtgaagctgg agctcttgcg cgaggcggaa tttgttctcc
     3301 agcatgagaa ccgaagttgg atgcggctaa tgatcgaggc ggagtggttt gggtagcggt
     3361 tgcgccacgt tcctcgccgc ccggcgttcc gggggggcca ggccggtccg cggctgagcg
     3421 ccttggtggc cctgctgacg gcttgctgct gcgcggagcg ttcgccggtt cggcctggtc
     3481 gaggaattct cgaaccatcg cgagtggctg tggaccttcg tggatgtgga aggcgtcgag
     3541 ccgaccaaca acgccggcca acgcgccttg cggcatgccg tgatctgggg gaaactctgc
     3601 ttcggcacgc aaagcgaatc cggcagccgc ttcgtggaaa cgctcctgac gtcgtggaaa
     3661 cctgccgcca gcagttccgc ggcgcattcg ccttcgtggc cacggccgtc gaaactcatt
     3721 tcacccatcg ccccgcgccg tcactcgccg gggtgtgaac ggttactgcg tcgtggcagc
     3781 cgaattcgac acgatcgtcc aatggctcac aacgtgcgga gcctatccgc acaatcccgc
     3841 taccgtggag cggatcgaaa cgcacatttc ctgtgtttac ttggccggcg accaggttta
     3901 caagttgaaa aagccggtcc aatatgattt tctcgatttc tccacgtgcg aaaagcgcaa
     3961 gtacgcctgc cacgaggagc tgcggctaaa tcgacgattg gcgccacagg cgtatttgga
     4021 cgtcgtgccg atcacgcgct ccagtgataa ctcgctccaa attggcggcg gtggcgagcc
     4081 cctcgactgg ctggtccaca tgtggcggtt gccgaccgat cagatgctcg attcgctgca
     4141 tcgccgcggc gaactgcggc gcgagcagat cgaccgcctg gcggaagtgt tgatagattt
     4201 ctatcactcg cttgagcccc tcccgatttc ggcggacgag taccgcgcgc gactcgagtc
     4261 ccacgtccga ggaaatctga gggaattgat ggcagtctcc catcatttgc caccgaggat
     4321 tgtcgaacgc acacacgcct tccagcttca acttctcaag ctgcgcccag aaatgtttga
     4381 ccagcgcgtc gcgcaagggc ggattgtcga aggccacggc gacttgcggc ccgagcacat
     4441 ctgtctgatc gagccgcccg caatcttcga ctgcattgag ttcaatgccg gattccgtca
     4501 attggatgtg gcggacgagt tggccttcct ggcagctgaa tgtgatattt tgggtgccga
     4561 gtgggtgggt ttgcggctgc tcgagaagta tcgcgagctg tccggcgatc agccgctgga
     4621 aattctgctc gacttctaca aatcgtaccg ggcttgcgtg cggggtaagg tcgccgcgct
     4681 gcgggccgac cagctcagtg gcaaggatcg cgaagcggct gctgctgaag ccctgcggca
     4741 cgtgcagctc gcagaccggt gtgtgtccca atgggccaga ccgctcgtgt taatcgtcgg
     4801 cggcctgtcg gggactggca aatcgacgct tgccggtgcg ctggctgaat ccctcggcgc
     4861 cgagctgctg cggaccgatg tgatccgccg cgagacaatc gccgtggagc agccgcaaaa
     4921 ccgctatagc gcagaatctc gccagcgggt ctatgacgcg atgttcgacc gcgccagccg
     4981 cttgcacgcg gaaggattat cggtcgtgct ggatgggaca ttcggttcga ggcaatcatt
     5041 cgtcggcgcg gtgcaaatcg caaagcatcc gcgcaccgta gtgttcgcgg tggaatgcga
     5101 gtgtccgccc gaagtcgccc gggagcgaat cagtcggcgg ttggcccgcg agaacgatcc
     5161 ctcggaagcc cgtccggatt tgcccggccg acaacaacag aactgggaag cgtggcctgc
     5221 cgacgttgct catgttcgca ttgacacgct ccagaccatc gacgagcagg tgcgaatcgt
     5281 tcagaacgaa ctggcgggtc gatggacggt ttgaggcagc gtcctgggcg agcttccgtt
     5341 cgaatccgtt cctgctacag tcccatcact ttccatcagc ggaccgatac ggcgacagcc
     5401 agacgcggca cagcggccga tcggctcgct tgtagccgaa cggctggcga tctctgcgaa
     5461 cgtcgtagag cggcggcttg ccagaatctg agacgtactc agagagcggg gaagtcccgg
     5521 ggacgtcctt ctcgccgtct gcgagcgccc aaaagagcgg cggcgcatcc cgctgattcg
     5581 gcggaggccc ggtcgtgagc ctcgcagtcc cgccttcgtt gacgcgatag acgggcacga
     5641 catcctggcg tggacgatcg agggcgaaaa acgcgatctt ttcaaactcg gcctgccggg
     5701 cgtcctcagc gtcgcgccac gcgaagtccg ccgccgcgcc gttcttctcc gcgtcgtaaa
     5761 gcgggacggg cagcaccagc cgctcgtcgg ccagatccag gcggtacatg atctggttgt
     5821 agtcgtaccg cggcgtcttg tgctcgttgc cggaaaacgt gtgcgtgtac gttccctcga
     5881 aatagatcag gcggcctcct tcctgatcga agaacgggtg ttgcttgggg ttgtaaaagc
     5941 tgtacttctc gtgggtgacg atcttcgtgg cgaagaccca cgggccgacc ggcgaatcgg
     6001 cttcggcata ccatgtttcg ccgaggagcg acgtaccgta cagctcgcag acgatcagca
     6061 caaaccgctt acgaaacgcg ttccaacaca ccgatcctgc gtgggccgcg accggcttgc
     6121 tcgtctccgc gtcgcggagt tgcagcaggc cctcgtgggg cttcaacaag ccctttttcg
     6181 taagcctggc ttgctcggca gggccaatcg gcggcgtgtt ctttcgccag gcatagcggg
     6241 cccgctttag ctcgtcgcga tcgatctgcg ggtcgtccag acgcgttccc ggcttgaggc
     6301 aggtgaaggc ctcgtagcgc gtaaggtccc gaaaggcctc ggcagtggac ggcacgcgga
     6361 ccagtggaaa gggcgcagcg aagagggtcc aatcgccgcc agtcgtgtcc cgtagcggct
     6421 ggccgtcggg atggagcggc acgttcggct c
//
LOCUS       DASUGB010000702         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_495772, whole genome shotgun sequence.
ACCESSION   DASUGB010000702 DASUGB010000000
VERSION     DASUGB010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495772"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..650
                     /gene="rsfS"
                     /locus_tag="VFV87_13445"
     CDS             264..650
                     /gene="rsfS"
                     /locus_tag="VFV87_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5104817.1"
                     /translation="MAATLAQSVVRTSPDRSLQLAQAAAHEAEDNRGTNVVVLDMREQ
                     TSIFDYFVIATGTSNRQLRAMSDAIDDVLQKQFGHARVGLEGYEDSHWILLDYGSVVI
                     HMFDEATREYYQIEDLWAGAKRVEWK"
     gene            complement(747..1316)
                     /locus_tag="VFV87_13450"
     CDS             complement(747..1316)
                     /locus_tag="VFV87_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104818.1"
                     /translation="MTRCLSFLALFSMSAILVSAGVARADHGFSTVEDLAFHVQDHSR
                     DLYYEVRNHLGQTSAGRHLMSDVVEVNRAAARIRSYAHAGYNLEVICRDARKLDELIH
                     HMEEIVDDLRHAGHDHYGHDHFGYGQFGHGQFDGRHVASLLREIEEDVHNLQSEIEWL
                     ERSAVRPGVSVGPGGIYLGGRGFSIRLAR"
     gene            complement(1690..3108)
                     /locus_tag="VFV87_13455"
     CDS             complement(1690..3108)
                     /locus_tag="VFV87_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HEX5104819.1"
                     /translation="MYCGRTHGSRWAAALVLALGLVLALGAVPAVAQNNNNQGNVNNF
                     IGTLPAGVMVSPEGVLRVKQFTDRTGQLTRTRIAEAKARLDGGLAKASELRKVSLNRL
                     EAAIAERTAAGEAPTDEMKYLAGLTQIQYVFFYPDTNDIVVAGPAEGFFPDPSGRVLG
                     INSGRAVLELQDLVAALRAFPPAGKQTQVISVSIDPTQEGLQAMQRWLASIRPGPGDA
                     DKIVEGLKEKLGLHDVSVVGVSPRTHYAQVLVEADYRMKLIGIGIEPPPVKMATYIGK
                     ASPTEVSRNAMQRWFFTPNYDCVRVAEDNLAMQLEGQGVQLIGANELVQGDGTRAAAA
                     GDNPASKAFCASFTANYPQIAAKSAVYAQLKNLIDMAIAAAFIQKQDYYGQAGWKMEV
                     LGDEQQFPIEVYEAPKSVETACTAVWKGNRLMTPVGGGVRIEPLEALQASHVLQDEQG
                     AVKGARAKVSLGGLAKGQWWWD"
BASE COUNT          722 a         1283 c         1032 g          681 t
ORIGIN      
        1 tcgaccagag ctggtcgccc aagcttcaca aatggcggtg aacgcgccgc gtaggcggga
       61 gggggtcggg agtcaattgc ggtcaagacc aaccatcatc gacgatcgtt gccgcaattg
      121 actcccgacc ccttcggtct cctgtagtct tagaccccag ccctcatcct ttcccgaacc
      181 tttccgctga cttgcccgtc ccgtctgagt agaatgcaac cggtgggcac tcgcccgata
      241 aaccccaact gaaaggagcg tgcgtggcag ccaccctagc tcaatcggtc gtgcggacca
      301 gtcccgatcg cagcctgcaa cttgcccagg ccgcggccca cgaagcggag gacaaccgcg
      361 gaaccaatgt ggtggtgctc gacatgcggg agcaaacctc gatcttcgac tacttcgtca
      421 tcgccacggg caccagcaac cggcagctcc gggcgatgag cgatgcgatc gacgacgtgc
      481 tgcaaaagca gttcggccac gcccgcgtcg gcctcgaagg ttacgaggac agccactgga
      541 tcctgctcga ctacggctcc gtggtgatcc acatgttcga cgaagccacg cgcgagtact
      601 accagatcga agacctctgg gccggcgcca agcgggtgga gtggaagtag gacgggaaca
      661 cgacaaaccc caaaatgccg agaaccaggc gatgcggcac gcccgcatcg ccaagaccca
      721 aggatgcggc tcaatgcaac gggcggctac ctggcgagcc tgatcgagaa gcccctgcca
      781 ccgaggtaga tgccgccggg gccgacagaa actcccggcc gcactgcgga gcgctccagc
      841 cattcgattt cgctctgcag gttgtgtacg tcctcttcaa tttcccgcag cagactcgcc
      901 acgtgtcgcc cgtcaaattg cccgtgccca aattgcccgt agccgaagtg atcgtgcccg
      961 tagtgatcgt gccccgcgtg ccgcagatca tcgacgatct cttccatgtg atggatcagc
     1021 tcgtcgagct tccgggcatc gcggcagatc acttccaggt tgtagccggc atgagcgtac
     1081 gaacgaatcc gcgcggccgc gcgattgact tccacgacat cggacatcaa atgccggccg
     1141 gcacttgttt ggccgagatg attacggact tcgtagtaca ggtctcggct gtggtcctgc
     1201 acgtgaaagg ccaaatcctc gacggttgaa aagccgtgat cagcacgggc aacgccggcg
     1261 ctgacgagga tggcggacat cgaaaacaac gcgaggaagg aaaggcagcg ggtcatggca
     1321 tcgtccctac gtttgttggt ttgggtcatc cggctgagcg actggctgtc gctacctctt
     1381 cgccagacgg aaaactccca aacgcaagca cgatgccagc aaatccagtc caaaccttta
     1441 gctcgcgacg caactccttt ggcataatcg ccttacgttg tcatcgagat cgcatcagcc
     1501 aatggcacag cggcccgtcg cacagtcgca cgatcgagag tgacatctta tcgactacac
     1561 cagaaagtca aagcgacttc actgagacga gcaacaacgc ggccggcaaa tgccaagcga
     1621 ccgcagccga acttcagccg cagtcgctca gtgcaaacga ctcgcaaaga ttcgcgtcgc
     1681 ccagggcgac taatcccacc accactgccc cttggccagt ccgcccaggc tcaccttggc
     1741 ccgggcgcct ttcaccgcgc cctgctcgtc ctggagaaca tggctggctt gcagcgcctc
     1801 gagcggctcg atgcggacgc cgccgccgac gggcgtcatc aggcgatttc ccttccagac
     1861 cgccgtacac gccgtctcca ccgatttcgg cgcctcatac acctcgatcg ggaactgctg
     1921 ctcgtcgccc agcacttcca tcttccagcc cgcctggccg tagtagtcct gcttctgaat
     1981 gaacgcggcc gcgatcgcca tgtcgatcaa attcttgagc tgcgcataca ccgcgctctt
     2041 ggcggcaatc tgcggataat tcgccgtgaa cgaggcacag aacgccttgc tcgccggatt
     2101 gtcgccggct gccgcagctc gcgtgccatc cccctgtacc agctcgttgg cgccgatgag
     2161 ctgcacgccc tgcccttcca actgcatcgc caggttgtct tccgccacgc ggacgcagtc
     2221 gtaattcggc gtgaagaacc accgctgcat cgcattccgc gaaacttccg tcgggctggc
     2281 cttgccaatg tacgtcgcca tcttcaccgg cggcggctcg atgccgatgc cgatcagctt
     2341 catccgataa tccgcttcca ccagcacctg ggcatagtgc gtccgcggcg aaacgccgac
     2401 caccgacacg tcgtgcagcc ccagcttctc cttcagaccc tcgacgatct tgtccgcatc
     2461 gcccggaccc ggccggattg aagccagcca ccgctgcatc gcctgcagcc cttcctgcgt
     2521 cggatcgatc gagaccgaaa tcacctgcgt ttgcttgccg gctggcggaa atgcccgcag
     2581 cgccgccacc agatcctgca attccagcac agcccggccg gagttgatcc ccagcacgcg
     2641 gcccgaagga tccgggaaaa agccttccgc cggcccggcg acgacaatgt cgtttgtgtc
     2701 cggatagaaa aagacgtact gaatctgcgt cagcccggcc aaatacttca tctcgtcggt
     2761 cggggcctct ccggcggcag ttcgctcggc aatcgcggcc tccaggcggt taagcgaaac
     2821 cttccgtagc tcgctggcct tcgccaggcc gccgtccagg cgggccttcg cttccgcgat
     2881 tcgcgtccgc gtgagctggc cggtgcggtc ggtgaattgc ttcacccgca gcaccccctc
     2941 ggggctgacc atcacgcccg cgggcagtgt gccgatgaag ttgttgacgt tgccctggtt
     3001 gttgttgttt tgcgccacag ccgggaccgc tcccagcgcg agcaccagcc ccagggcgag
     3061 caccagtgcc gccgcccacc gcgacccgtg agttcttccg cagtacatgc gccggttcct
     3121 cttcgtgaat ggcccctgtc ggctggcagc cgaatgttcc gtcttccgat tactgccgct
     3181 cgaatcgctt ctcaatcgcg ccgcaacggt ccgtgcggct tgccaaatca aatctcaatt
     3241 ctatcgcaaa aaaccggctg ccaatcgtgc gcacccggga taccttgccg gaacgctccc
     3301 gcgccaggcg tcgcctggaa gcccgcccct gcgcgggaac gctggttgat cagttcggcg
     3361 acggtcgccg cagcccaagg cgaacgcctg ccgcggcaac gtcgcataat gccacctttc
     3421 cgatcttaat tgcccacttt ccccaacgca cggaattcca aattgccctc cgcgtgcctg
     3481 gcgagcggga ggaataagcc tcccggtacg gtcgccctct cgtttaacgg cgagccacag
     3541 gcgaaggcgt tgcaggccgg ccccttcacg ctgcgcaact ttttgaccgc tcaaaaacgc
     3601 gcaacgttcg aagttttttg tctcccatcc acccccgcgc aacatcgtcg taactcattt
     3661 aatgagaaac acttgcgtag cgcaacaccc cccgcatgag gatgttgcgc tgttgcgc
//
LOCUS       DASUGB010000703         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1189404, whole genome shotgun sequence.
ACCESSION   DASUGB010000703 DASUGB010000000
VERSION     DASUGB010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189404"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /gene="hflX"
                     /locus_tag="VFV87_13460"
     CDS             <1..1139
                     /gene="hflX"
                     /locus_tag="VFV87_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5104820.1"
                     /translation="LKSLVEHHDADVVIFDNDLAPSQTRNLEKALGVKVLDRTEVILD
                     IFANSAQTYEARLAVELAQLEYSLPRLKRMWTHLSRIKAGIGMRGPGEKQLEEDRRLA
                     LKRIHDLRTELHGIERRKERLVASRDDRLTVSLVGYTNAGKSTLMNALTGAGVLAADK
                     LFATLDTRTRRWQLPSWGPVLLSDTVGFIRDLPHTLVASFKATLEEARQADLLLHVAD
                     AANPAVLQQISAVYKVLQELGIEEKDTLLVLNKVDALADRADLAPVLARYPNAVPVSA
                     RTREGFDRLHVAVSDALSRSFADLEVETNVANGRMLAYLAAHGEVLDKRYSDEQVIVH
                     VRIPHRHLGPLHEPGTRFWPHDNGVPVAPDVAAAPITATAQDVA"
     gene            1246..2025
                     /locus_tag="VFV87_13465"
     CDS             1246..2025
                     /locus_tag="VFV87_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5104821.1"
                     /translation="MARRPIAGLRTIVTGASSGIGRALVIELVRGGAKVVAMARREDR
                     LKELQAEIADNSLLRTLAGDVTAQADRAAAIELAQREFGGLDALVNNAGIGALGRFDE
                     ADEARLRQVMEVNFFAPALFLREALPALSAGNRPIVVNVSSVLGHRAVPEKSEYCASK
                     FALHGLSDALRIELASRGIDVLLVSPSTTVSEFFERAEGQSSRKHFRFGAMSAEAVAL
                     QTVAAIRRGRREIILSAGGNLLVWIDRLCPPLADWFVARWV"
     gene            2190..>2676
                     /locus_tag="VFV87_13470"
     CDS             2190..>2676
                     /locus_tag="VFV87_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104822.1"
                     /translation="MIGSNWPLALPVNARSLAAGGCCLAALFAGDLLSAAEPPKPSLI
                     QQYLQAKTGVADSPAISASEAEAAESTPASASDKSEVAPGEIPFVEARPIQSAAAGAS
                     PILPIAKAAQPDDESGLPPIVTPDMLAPPARRAPRASRTPLPELAPPRAAPAMLRPQP
                     LT"
BASE COUNT          458 a          951 c          850 g          417 t
ORIGIN      
        1 agctcaagtc gcttgtcgaa caccacgatg ccgatgtcgt aatcttcgac aacgacctgg
       61 cccccagcca gacgcgcaac ctggagaagg ccctcggcgt caaagtcctc gaccgcaccg
      121 aagtgatcct cgacatcttc gccaatagcg cccagaccta cgaagcccgc ctcgcggtcg
      181 agttggccca gcttgaatac tcgctgccac ggctgaaacg gatgtggacg cacctctcgc
      241 gcatcaaggc cggcatcggc atgcgcggtc cgggcgaaaa acagctcgaa gaggaccgcc
      301 ggctggccct gaagcggatt cacgacctgc ggaccgagct gcacgggatc gaacgccgca
      361 aggagcggct ggtcgcctcg cgcgacgacc ggctgacggt ctcgctggtc ggttatacga
      421 acgccggcaa gagcacgctg atgaacgccc tgaccggcgc gggcgtgctg gccgccgaca
      481 agctctttgc cacgctcgac acccgcaccc gccgctggca actgccgagc tggggccctg
      541 tgctgctgtc cgacaccgtt ggcttcatcc gcgatctgcc gcacactctg gtggcgagct
      601 tcaaggcgac gctggaagag gcccggcagg ccgatctgct gttgcacgtg gccgatgccg
      661 ccaatcccgc cgtgctgcag cagatcagcg ccgtctataa ggtgctgcag gagctgggca
      721 tcgaggaaaa ggacacgctg ctggtgctca acaaggtcga tgccctggcc gaccgggcag
      781 atctcgctcc ggtcctcgcc cgttatccca acgccgtgcc ggtcagcgct cgcacgcgcg
      841 agggcttcga ccggcttcac gtggctgtca gcgacgccct cagccggtcg tttgccgacc
      901 tggaggtcga gacgaacgtg gccaacggcc ggatgctggc ctatcttgcg gctcatggcg
      961 aagtcctcga caagcgctac agcgacgagc aagtgatcgt ccacgtccgc attccgcacc
     1021 ggcaccttgg cccgctgcac gaaccgggga cgcggttctg gccgcacgac aacggtgtgc
     1081 ccgtcgcgcc ggatgtcgcc gccgctccca tcaccgccac agctcaggat gtagcctgag
     1141 aggctggcat gcgtcgtagc cgcgttcgcc agaacgcggg aagcctggcc gcccgcgccc
     1201 gcactctggc gagtgcggct accgttcaga gactgctttt aggtcatggc ccgacgcccg
     1261 atagccggcc tgcggacgat cgtcaccggc gcatccagcg gcatcgggcg ggcactggtg
     1321 atcgagctgg tccgcggcgg ggcaaaggtc gtcgcgatgg cccggcgcga agaccgcctg
     1381 aaggaactgc aagcggaaat tgccgacaac agcctgctcc gcacgctggc cggcgacgtg
     1441 acggcacaag ccgaccgggc ggcagcgatc gagctggccc agcgcgagtt cggcggcctc
     1501 gacgccctgg tcaacaacgc cggcatcggc gcgctgggcc gctttgacga ggcggacgaa
     1561 gcccgcctgc gtcaggtcat ggaggtcaac ttcttcgcac cggccctgtt ccttcgcgaa
     1621 gccttgccgg ccctctcggc ggggaaccgg ccgatcgtcg tcaacgtcag ctcggtgctc
     1681 ggccaccggg ccgtgccgga aaagagcgag tactgcgcga gcaaattcgc gctgcacggc
     1741 ctgtccgacg ctctgcggat cgagttagca tctcgcggca tcgacgtgct cctcgtcagc
     1801 cccagcacca ccgtcagcga attcttcgag cgggcggagg gccagtcctc gcgcaagcac
     1861 ttccgcttcg gcgcgatgtc ggccgaagcc gtcgccctcc agacggtcgc cgccatccgc
     1921 cgcggccggc gcgagatcat cctctccgcc ggcggcaact tgctcgtgtg gatcgaccgg
     1981 ctgtgtccac cgctggctga ctggttcgtc gcccgctggg tctgagccgc tactgtccgc
     2041 acaatccgga ttacccgcac aatttctcaa ctgcagtcct ggcctctccc gataggtctt
     2101 gatgggtgcg gctgcgcagg caacgacgct ccagccgccc caaggcaggg ggcaacgtgc
     2161 cgcctgcgtg cctcgtgggg ggagtagcaa tgatcggaag caactggccg ctagctctgc
     2221 cggtaaacgc acgctcgttg gccgccggcg gctgctgtct tgcagcgctc ttcgctggcg
     2281 atctgctgag cgccgccgag ccgccaaaac cgagcctgat tcagcagtat ctgcaggcca
     2341 aaactggagt cgccgattcg ccggcgattt ctgcatccga agccgaggcc gcagaatcaa
     2401 ctccagccag cgctagcgac aagtccgaag ttgcgcccgg cgaaatcccg ttcgtcgaag
     2461 cccggccgat ccaatcagcc gcagcgggcg ccagccccat cttgccgatc gccaaggcgg
     2521 cgcagccaga cgacgaatcc ggattgccgc cgattgtcac gccggacatg ctcgcgcccc
     2581 ctgctcgccg cgcgccgcgg gcatccagaa cgcccctgcc ggaacttgct ccgcccagag
     2641 cggcgccggc catgctgcga ccacaaccac tcacgg
//
LOCUS       DASUGB010000704         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1607214, whole genome shotgun sequence.
ACCESSION   DASUGB010000704 DASUGB010000000
VERSION     DASUGB010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607214"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFV87_13475"
     CDS             <1..1173
                     /locus_tag="VFV87_13475"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104823.1"
                     /translation="QICEHLPLLAERSHLWALVRSLTHPSNDHSLGHHIMLTGRSPAP
                     VGFSPNGPQRTDFPSIAAIAGAATLVRNNLPPAVVLPERLVHYSGRVIPGQFAGQMGS
                     HRDPWFIEASPFHNTSYGAYPTYKFDHQERGNKDDRVFQAPNLSLPQGIDPPRFHDRT
                     GLLASLQQQREALDRAAQTQTFDSLRQGVISLLTDAKVKHAFDVTNAGEKTQERYGKN
                     SFGWSLLMARRLVEAGVNLVQVNLGNNESWDTHGEAFPHLKEKLLPPTDRALSALLDD
                     LNESGLLDSTLIVMAGEFGRTPKITHLPQYYKLPGRDHWGAVQTVFFAGGGVKGGTVI
                     GSSDRLGGYPASSPHTPENMAATIYRSLGIPTTAAWHDEQDRPHQIYYGEPIAGLT"
     gene            complement(1200..1958)
                     /locus_tag="VFV87_13480"
     CDS             complement(1200..1958)
                     /locus_tag="VFV87_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5104824.1"
                     /translation="MPLTISQYLLVSLILFFSSVVQGAVGFAAGLFGIPLLMLTGVSL
                     PDAVAISLVAAAVQNVAAAWQLRREIDYRLAVRPMLIRFATLPLGVWALSLIGSEQKD
                     LAGQLVGVVVLAIVAVQSALRIEPRERIHQAWEWLAFGMGGFLLGLCGMGGPAMVLWV
                     MAHNWPMNRARGFLFFIFATGIPLQALLLWLAFGNPILEAMLLGLVALPSVALGLYVG
                     LWLAHIAPDRVLRILSWAVLVLIAVSSMVMPYVT"
     gene            2037..2921
                     /locus_tag="VFV87_13485"
     CDS             2037..2921
                     /locus_tag="VFV87_13485"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5104825.1"
                     /translation="MANSPIGLIGCGLLGSALAERMMAGGLPLVGFDRDLAASDRLRQ
                     LGAEPVASAVEVARTAAAIVLCLPDSHVVVAVVDQLAAALKPGALLIDATTGDPDRTA
                     ALADRLSASGIGYIDATIAGSSDQARRGEAVVIVGGRPADVERAQPVLRTWSDRQFHV
                     GPPGSGARMKLVVNLVLGLNRAVLAEGLSLAEASGIELARALEVLHATPAYSAAMDTK
                     GPKMVSGDFSPVARLAQHLKDVRLILALAERHAAHTPLSNVHEELLKRAVELGLGEAD
                     NSAIIRAFRPARQQHGEA"
BASE COUNT          569 a         1005 c          954 g          461 t
ORIGIN      
        1 cagatttgcg agcatctgcc gctgctggcc gagcggagcc acttgtgggc gctggtccgc
       61 tcgctgacgc atccctcgaa cgatcattcg ctcgggcatc acatcatgct caccggccgg
      121 tcgcccgcgc cggtcggctt cagcccgaac gggccgcagc ggacggactt tcccagcatc
      181 gcggcgatcg ccggggcggc gacgctcgtc cgcaacaatc tgccgccggc ggtcgtgctg
      241 ccggagcggc tggtgcatta ctcggggcgc gtgattcccg gccagttcgc cgggcagatg
      301 ggttcgcacc gcgatccgtg gttcatcgag gcctcgccgt tccataacac gtcgtacggg
      361 gcgtatccga cctacaagtt cgatcaccag gaacggggca acaaggacga ccgcgtattt
      421 caagcgccga atctgtcatt gccgcagggc attgacccgc cgcgcttcca cgaccgaacc
      481 ggcctcttgg ccagtcttca gcagcaacgg gaagccttgg accgagcggc tcaaacgcag
      541 acgttcgaca gcctccgcca gggcgtgatc tcgctcttga ccgacgccaa ggtgaaacac
      601 gccttcgatg tgaccaacgc cggcgagaag acgcaggagc gctatggcaa aaacagcttc
      661 ggctggtcgc tgctgatggc gcggcgcttg gtggaggcgg gtgtgaacct ggtgcaggtc
      721 aacctgggga acaacgaatc gtgggacacg catggcgaag cctttccgca cctcaaagag
      781 aagctgctgc cgccgaccga ccgcgcactt tcggctttgc tcgacgacct gaatgaaagc
      841 ggtctcctcg atagcacgct gatcgtcatg gccggcgaat tcggccgcac gccgaagatc
      901 acgcacttgc cgcaatacta caagcttccc ggccgcgacc actggggggc cgtacagacg
      961 gtgttcttcg ccggcggtgg cgtgaaaggg ggcacggtca tcggctcgtc ggaccgcctc
     1021 ggcgggtatc ccgcttccag cccccacacg cccgaaaata tggccgccac gatttaccgc
     1081 tccttgggca tccctaccac ggccgcctgg cacgacgagc aggaccgccc gcatcagatc
     1141 tactatggcg agcccatcgc cgggctgacc tgacgtgggg cgggcgtctc gccggcccgt
     1201 caagtcacgt agggcatcac catcgagctg actgcaatca gcacgagcac agcccagctc
     1261 aaaatgcgca gcacgcgatc gggggcgata tgggcgagcc acagaccgac gtaaagccca
     1321 agggcgaccg acggcagcgc caccagcccc agcagcatcg cctccagaat gggattgccg
     1381 aacgcgagcc acaagagcag ggcctggagc ggaatgccgg ttgcaaagat aaaaaacaaa
     1441 aacccccgcg cccggttcat cggccagttg tgcgccatca cccacaggac cattgccggc
     1501 cctcccatgc cgcacaggcc gagcagaaat ccgcccatgc cgaacgccaa ccactcccag
     1561 gcctggtgaa ttcgctcgcg cggctcgatg cgcaaggcgg attgcacggc gacgatcgcc
     1621 agaacgacga caccgacgag ctggccggca agatcctttt gctccgatcc gatcaacgac
     1681 agcgcccaca cgcccagcgg cagcgtggca aagcggatca gcatcggccg cactgccagg
     1741 cgataatcga tttcccgccg caattgccag gcagcagcga cattttgcac cgcggctgcg
     1801 acgaggctga tcgcgaccgc gtccggcagc gaaacgccgg tgagcatcag gagagggatg
     1861 ccgaatagtc ccgcggcaaa gcccacggcg ccttgcacga cgctgctaaa aaacaaaatc
     1921 aggctgacca gcagatactg cgaaatcgtc agcggcatgg agaatggagg gttgcagtcc
     1981 cggcgaaccg gcagcagccg ggccgcgtag tgctggtcgc accaggatag tcccccatgg
     2041 caaattcacc aattgggctg atcggctgcg ggctgttggg ctcggcattg gccgagcgga
     2101 tgatggctgg cggcctgccg ttggtcggat tcgaccgcga ccttgccgcc agcgaccggc
     2161 tgcggcagct cggggcggag ccagtggcct cggccgtgga agtcgcccgc accgccgcgg
     2221 ccatcgtcct ctgcctgccc gattcgcacg tcgttgtggc tgtcgttgat cagctcgcag
     2281 ccgccctgaa gccgggagcg ctcctcattg acgcaaccac cggcgacccc gaccggaccg
     2341 ccgctttggc cgatcggctt tcagcgagcg gaatcggcta catcgatgcg acgatcgccg
     2401 gctcgtccga tcaggcccgc cgcggcgagg cagtcgtgat cgtcggcggg cgccccgcag
     2461 acgtggagcg cgcccagccg gtcttgcgga cgtggagcga ccgccagttc cacgtcggtc
     2521 cgcccggcag cggagcgcga atgaaactcg tcgtgaacct tgtcctgggc ttgaaccgcg
     2581 cggtgcttgc agaagggctg tcgctcgccg aggcgagcgg catcgagctc gcgcgggcct
     2641 tggaagtgct ccacgccacg cccgcctatt cggcagcgat ggacacaaag ggccccaaaa
     2701 tggtcagcgg cgatttctct ccggtcgccc gcctggccca gcacctgaag gacgtgcggc
     2761 tgatcctggc gcttgccgag cgccacgccg cccatacgcc gctttccaac gtgcatgaag
     2821 aattgctgaa gcgcgcggtg gaactgggac tgggcgaagc cgataatagc gccatcatcc
     2881 gggcctttcg gccggctcgt cagcagcatg gggaagcatg atttcggcag tcgaactaac
     2941 gcgacggttc gatacggggg ggcacgatgt gctcgcagtc gatcgcatc
//
LOCUS       DASUGB010000705         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_218147, whole genome shotgun sequence.
ACCESSION   DASUGB010000705 DASUGB010000000
VERSION     DASUGB010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218147"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFV87_13490"
     CDS             <1..563
                     /locus_tag="VFV87_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104826.1"
                     /translation="PSAYDARDANASGVRDFGKGCLLARPLVEVGVPLVEVVLQSWDT
                     HANNLPTTRRNCEALDPAWSALVEDLDERGLLDNTLIVWMGEFGRTPRLNPGANGGGA
                     GRDHYGRAWSTVLMGGGIKGGQVIGRTDKDGATVEDRPVSAADFMATICQALGIDWQK
                     LNDGPGGRTVRVTAAGAQPITELLPS"
     gene            complement(541..2226)
                     /locus_tag="VFV87_13495"
     CDS             complement(541..2226)
                     /locus_tag="VFV87_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104827.1"
                     /translation="MQVRKLIAAGYALAFAGLAAAAGASAAETPPKPPLFEGLGTVGR
                     TVTTASPEAQRYFNQGLCFLFAFNHDEAIRSFRQAAELDPSCAMAWWGVSLANGPHIN
                     NPVVPPERAKAAWEALAKARETAAKASQTEQALIAALAKRYADPQPEARKPLDEAYAA
                     AMRDVWKAHPDEADIGALFAESLMDLRPWDLWQEDGQPQPGTEEVVSTLEAVLKIAPN
                     HPLALHLYIHAVEASPQPERAVDEADRLRNLQPGLGHLVHMPSHIDVRLGTWAKAIAA
                     NQKAIAADRKYREQSPEQGFYRLYMAHNHHMLAYGAIMRGQSELAISSINDMAKGIPP
                     QWIKDNAGLADGFTAMPLEILVRFGRWDEVLAVPEPPEFLPIARALRHCSRGIALAAQ
                     GEIEKAKIEQAAFLAAQAKLPEEAHFGNNTGADILAVAEHLLAGEIRYREGKTEIAFA
                     ELREAVKREDALRYDEPPDWIHPVRHALGAILLAEGKAAEAEKVYRSDLAKLPHNGWA
                     LFGLARSLEMQGQRDAAAKVQAQFEQVWSDADVTITSSCYCQPARAVTKAATP"
     gene            2439..>3089
                     /locus_tag="VFV87_13500"
     CDS             2439..>3089
                     /locus_tag="VFV87_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5104828.1"
                     /translation="MHRLILCLFLLIAIPRATRAGEPDVQHVVLVSIDGLAASYLTDQ
                     RAELPTLRKLMAEGASAQGMITSFPSVTWPSHTSLITGVQPARHGVIGNNVWDRKNNR
                     ALTYIGDPELTKDQAVRVPTLYDHAHEAGLTCGAVIWQCVNGAKSLDWAIPDSNKPEL
                     HAKYTTPGFVAEMTKAGIDATKLGQWGWNKERSTDRDILYTKVGNYLLEKHGVNLLL"
BASE COUNT          539 a         1025 c          983 g          542 t
ORIGIN      
        1 cgcccagcgc ctacgacgcc cgcgacgcaa atgccagcgg cgtgcgcgat ttcggcaagg
       61 gctgcctcct ggcccgcccg ctggtggaag tcggcgtccc tttagttgag gtcgtgctgc
      121 aaagctggga cacgcacgcc aacaacttac ctacgacacg ccgcaactgc gaagccctgg
      181 acccggcctg gagcgcgctc gtggaggatc tggacgagcg cggccttctg gacaacacac
      241 tgatcgtctg gatgggcgaa ttcggccgca caccgcgtct gaatccgggg gccaatggcg
      301 gcggcgcggg ccgcgaccat tacggccggg cctggagcac cgtgctgatg gggggcggca
      361 tcaaaggcgg ccaggttatc ggccgcacgg ataaggacgg cgcaaccgtc gaagaccggc
      421 cagtgagtgc cgcggacttt atggcaacga tctgccaggc gctcggcatc gactggcaga
      481 agctcaacga cggtcccggc ggccgcacgg tccgcgtgac tgccgccggc gcccagccga
      541 tcacggagtt gctgccttcg tgaccgccct tgccggctgg cagtagcacg acgaggtgat
      601 cgtgacgtcg gcgtcgctcc agacttgctc aaattgagct tgcaccttgg ccgctgcgtc
      661 tcgctggcct tgcatctcca ggctgcgggc cagaccgaaa agcgcccagc cgttgtgggg
      721 cagcttcgcg aggtcgctgc gatagacctt ctcggcttcg gcagctttac cttcggcgag
      781 caggatcgcg ccgagagcgt ggcgaacggg atgaatccaa tcgggcggct cgtcgtaacg
      841 gagggcgtcc tcgcgcttca ccgcttcgcg cagctcggcg aaggcgatct ccgttttccc
      901 ttcgcgatag cgaatctcgc ctgccaggag gtgctcggcg acggccagaa tgtcagcgcc
      961 cgtgttgttg ccgaagtggg cttcctcggg cagtttggcc tgcgcggcca gaaacgcggc
     1021 ctgctcgatt ttggcctttt cgatttcgcc ctgggcggcc agggcgatcc cgcgcgagca
     1081 gtgccgcagg gcccgggcga tcggcagaaa ctcgggcggc tccggcacgg ccagcacttc
     1141 gtcccagcgg ccgaagcgga ccaggatctc cagcggcatc gccgtaaagc cgtcggccag
     1201 gccggcattg tccttgatcc actgcggggg gattccctta gccatatcgt tgatggacga
     1261 aatggccagc tcgctctggc cgcgcatgat cgctccatag gcgagcatgt gatggttgtg
     1321 ggccatgtag aggcgataga aaccctgctc gggcgactgc tcgcggtact tccgatccgc
     1381 cgcgatcgcc ttctggtttg ccgcgatcgc cttcgcccac gtccccagcc gcacgtcgat
     1441 gtgcgacggc atgtggacca gatgccccag gccaggctgc agattccgca ggcggtcggc
     1501 ttcgtccacg gcccgctcgg gctggggcga ggcttccacc gcgtggatat aaaggtgcag
     1561 cgccagcgga tgattcggcg cgatcttcag caccgcttcg agcgtgctga caacttcctc
     1621 ggtccccggc tgcggctggc catcttcttg ccacaaatcc cagggacgca ggtccatcag
     1681 cgactcggca aacagggcgc cgatgtccgc ttcgtccgga tgggccttcc aaacgtcgcg
     1741 catcgccgct gcatacgctt catccagcgg cttgcgggcc tccggctgcg ggtcggcata
     1801 acgcttggcg agcgccgcaa tcaatgcctg ttcggtctgg ctcgccttgg cagccgtctc
     1861 gcgagccttg gccagtgcct cccaggcggc tttcgcccgc tcaggcggca caaccgggtt
     1921 attgatatgc ggcccattcg ccagcgaaac gccccaccag gccatcgcac agctcgggtc
     1981 gagctccgcc gcctgccgga acgagcggat cgcctcgtcg tgattgaagg caaacaggaa
     2041 gcagaggcct tgattgaagt atcgctgtgc ttcgggcgaa gcggtcgtca ccgtgcggcc
     2101 gaccgtgccc agcccttcaa atagcggcgg tttgggcggc gtctcggcag cggaagcgcc
     2161 cgctgctgcc gccagtcctg cgaaggccaa agcatagccg gctgcaatta gtttcctcac
     2221 ctgcatctct gtctcctgaa gcgaaagacg atcccaccgg acaaaaaagg ctcgccgcgc
     2281 aaacgtcgcc cagcggccga tcgcaagtta gtttatccta aacattccca tgccaaaccc
     2341 ggggtttacc agcagtcaac caagcgctgg cggcttcgct tgaacccgat ttggcggtaa
     2401 cgtaggtcct cgcgaatccc tcgacataag gcccgactat gcatcgcctg attctctgcc
     2461 ttttcctcct gatcgcgatc ccgcgggcca cccgcgccgg ggaaccagac gtgcagcacg
     2521 tcgtcctcgt cagcatcgac ggcctggcgg ccagctattt gaccgaccag cgggctgaac
     2581 tgccgacact gcggaagctg atggctgaag gcgcctcggc ccagggcatg atcacgagct
     2641 ttcccagcgt cacctggccc tcgcacacgt cgctgatcac gggcgtgcag ccggcgcggc
     2701 acggcgtgat cggcaataac gtctgggacc ggaagaacaa ccgggccttg acctacatcg
     2761 gcgatccgga gttgacgaag gaccaggccg tccgcgtgcc gacgctttac gaccacgccc
     2821 acgaagccgg tctgacctgc ggggccgtca tctggcagtg cgtcaacggc gccaagtcgc
     2881 tcgactgggc gattcccgat tccaacaagc ccgaattgca cgccaaatac acgacgcccg
     2941 gctttgtggc ggagatgacc aaggctggca ttgatgcgac gaagctcggc cagtggggct
     3001 ggaacaaaga gcgttcgacc gaccgcgata ttctctatac caaggtgggg aactacctgc
     3061 tggagaagca cggcgtcaat ctgctgctg
//
LOCUS       DASUGB010000706         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_356347, whole genome shotgun sequence.
ACCESSION   DASUGB010000706 DASUGB010000000
VERSION     DASUGB010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356347"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="VFV87_13505"
     CDS             complement(<1..2048)
                     /locus_tag="VFV87_13505"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX5104829.1"
                     /translation="MPKSATPKQPQPQHKTTWAQLTWDELETWAGSSSLKRGKRYASA
                     GRVSDLTIAEDQSLHSKVRGTARYTVQVPAPRGGRLTDSSCSCPVGYRCKHAVATIVA
                     YLDALAGGTPVPAAKADDRRRKSVRKAHEVDEGDGWREGEIGEHAGYDDENDDDEDSG
                     EPTTWSSRRRQKTAPRAAAAQSKSQSLRAIVAAKDRGQLIDLVLEFALRHPELAKRLT
                     DESLLASGDATALIEATRRELRLLTAKQSWWHHWERRGSLPNFGPLQEKFERLRQLGL
                     ANHLLQLGAELLERGIRQVEQSDDEGHVAGEIRSCLDLAFDALPDSGLGGSEQLLFAI
                     YAMLVDDFDLCAGARQVLDRDLRAADWSQAADVLLERIRRPQELAAGGEFRRMRLVDF
                     AVRALQSAGRVGEILPLAEHEARTHHEYQRLITLLFERTDLPAAEAWLRTALQNTDPR
                     LHGLVAGYRRQLCEIATRRKDWPTVAGHAAELFFEQPSVETLQELLRSAAKAGCEGPV
                     RAGALLFLETGARPAGGSAIKTGNEPPREHSWPLTAPDYIRVSAPAKSKTNRAMRAFG
                     ANRPRPDVLLDLAMAEKRPDDALALYDRHFSQEAVRARGMDYIARHAPSVAQFVAPTH
                     PKRAAELYEALILANVNQVGESFYEQAVAYLKAMRPITAALGAPERWDSLMARIRNEQ
                     R"
     gene            complement(2082..2534)
                     /locus_tag="VFV87_13510"
     CDS             complement(2082..2534)
                     /locus_tag="VFV87_13510"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5104830.1"
                     /translation="MQAAIDLALAGVAAGRGGPFGCVIVKDDQIVGRGCNQVTSTNDP
                     TAHAEVVAIRDACARLGSFHLAGCELYASCEPCPMCLAAIYWARLDRLYFACTAADAA
                     AIGFSDEFICGQLALPAAARCVPAIPLMRDEALAAFAEWQAKPDRVPY"
     gene            complement(2682..>3836)
                     /locus_tag="VFV87_13515"
     CDS             complement(2682..>3836)
                     /locus_tag="VFV87_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX5104831.1"
                     /translation="ISTVNCSASVTRYIAQHFTPERLRDFPGVDGVLAIKHALGCGMQ
                     YQGLQHELLNRTLAGMARHPNIGGYLLVGLGCEQGAMGYLLQSQNLMQIGGNGKASGP
                     PLLSMQDLGGTAKTVAEGVRLVEQLLPQVNAARRETLPASEIVLGTNCGGSDGNSGVT
                     CNPALGIASDMIVACGGTSALAETTEIYGAEHLLTRRAVTAAVAEKLLARIQWWLWHT
                     GLYGVEIDNNPSVGNKEGGLTTIAEKSLGAVAKAGSTAMTEVYQYAEPITRKGMVVMD
                     TPGFDPPSVTGLVAGGCNMMVFTTGRGSCFGLKPCPTIKVASNTPMYERMIDDMDLNA
                     GEILAGRSVEDVGRELFEKILAVASGEQTKSEHLGYGDEEFVPWQVGPTL"
BASE COUNT          616 a         1262 c         1284 g          674 t
ORIGIN      
        1 cgctgctcgt tgcgaatgcg ggccatcagg gagtcccatc gctccggcgc gccgagggcc
       61 gcggtgattg gcctcatcgc tttgagatag gcgaccgcct gctcgtagaa cgactctcca
      121 acctggttga cgttcgcaag aatcagggcc tcgtacagtt cagccgcccg cttggggtgc
      181 gtgggagcga caaactgggc gacggacgga gcatggcggg caatgtaatc cattcctcgg
      241 gcgcggactg cttcttgcga gaagtgccgg tcgtagagtg ccaacgcgtc gtcgggccgc
      301 ttctcggcca tggccagatc gaggagcaca tcgggccgcg ggcgattggc gccaaatgcc
      361 ctcatcgcgc ggttcgtctt gctcttggcg ggcgcactga ctcgaatata gtccggcgcc
      421 gtcagcggcc aggaatgttc tcgcggcggc tcgttgcccg tcttgatggc agaaccgccc
      481 gctggtcgag cgcccgtttc cagaaagagc agggcgccgg cccgtaccgg gccttcacac
      541 ccggctttgg cagccgatcg aagcagctcc tgcaaggtct cgaccgaggg ctgctcaaag
      601 aacaactcgg cggcgtggcc ggccacggtc ggccagtcct tgcggcgagt ggcgatctcg
      661 cacagttgcc ggcgatagcc ggccaccaga ccgtgcagcc gcggatcggt gttctgcaag
      721 gctgtccgca gccaggcctc ggccgccggc aggtctgtcc gctcgaaaag cagcgtgatg
      781 agccgctggt attcgtggtg cgtgcgggcc tcgtgctcgg cgagcggcag gatttcgccg
      841 actcggccgg ccgactgcag cgcacgcaca gcgaaatcca ccaagcgcat tcgtcggaat
      901 tcgccgccgg cggctagttc ctggggacgg cggatgcgct cgagaagtac gtccgctgcc
      961 tgcgaccagt ccgccgcccg aaggtcgcga tcgagcacct ggcgagcgcc cgcgcacaga
     1021 tcgaaatcat cgacgagcat ggcgtagatg gcaaacagca gctgctcaga cccgccgagg
     1081 ccggaatccg gcagtgcgtc aaaggccagg tccaaacagc tgcggatttc gccggccacg
     1141 tgtccttcgt cgtccgactg ctcgacctgg cgaatgccgc gctcgagcag ttcagcgccg
     1201 agctgcagca agtgattggc caggccaagc tgccgcaaac gctcgaactt ttcctgcagt
     1261 ggaccaaaat tgggcaaact gccgcggcgt tcccagtgat gccaccagga ttgtttcgcg
     1321 gtgagcaagc ggagttcgcg ccgcgtggcc tcgataagcg cggtcgcgtc gccggatgcc
     1381 aggagcgatt catccgtcag ccgcttcgcc aattcgggat gccggagggc aaactccaag
     1441 accaaatcga tgagctggcc gcggtccttc gctgcgacga tcgcgcggag cgattgcgac
     1501 ttggattgtg cggcagcggc tcgcggcgcc gttttctgcc ggcgacggct cgaccaggtg
     1561 gtgggctccc cactatcctc gtcgtcgtcg ttttcgtcgt cgtacccggc atgttcccca
     1621 atttcgcctt ccctccagcc atcaccttcg tcaacttcgt gcgctttgcg caccgacttt
     1681 cgacggcgat cgtcggcctt ggctgcgggc accggcgtcc caccggccag cgcgtccagg
     1741 taggccacga tcgttgcgac tgcgtgtttg cagcgatagc caacgggaca agagcaactc
     1801 gaatccgtaa ggcgcccgcc ccggggtgca ggcacctgca ccgtatagcg cgccgtgccg
     1861 cgcaccttcg aatgcagcga ctgatcctcg gctatcgtca aatcgctgac gcggccggcg
     1921 ctggcgtatc tcttgccccg cttcagggaa ctcgagccgg cccaggtttc cagttcgtcc
     1981 cacgtaagct gcgcccaggt ggtcttgtgt tgcggctggg gctgttttgg cgtcgcggac
     2041 ttgggcatgc gcgatcctcc gtgaatgggg ctcggagccg gtcagtaggg tacccgatcc
     2101 ggcttcgcct gccactcggc aaaggcggcg agggcttcgt cgcgcatcag cgggatcgcc
     2161 gggacgcagc gggcggctgc cggcagggcg agctgcccgc agatgaattc gtccgaaaag
     2221 ccgatggccg ccgcgtcggc cgcggtgcag gcgaaataca agcggtccag gcgggcccaa
     2281 taaatggcgg ccagacacat ggggcacggc tcgcagctcg catacagctc gcaccccgcc
     2341 aggtggaacg agcccagccg cgcgcaggcg tcccgaatgg cgacgacctc ggcatgcgcc
     2401 gtcggatcat tggtcgaagt gacctggttg cagccgcggc cgacgatttg gtcgtccttg
     2461 acgatcacgc agccgaacgg cccgccgcgg ccagcggcga caccggccag cgccaggtcg
     2521 atggcagcct gcatgaacga cgcggggcta ggggctcggg attcggggct agggggcgac
     2581 tgcatggttt caagctgcgc cgaaagtctc agccccgaac tccgaatccc gatccccgat
     2641 ccccgatccc gaaccccgaa tcccgatccc cgaatcccgt tctacagcgt cggtcccacc
     2701 tgccagggca cgaactcctc gtcgccgtag cccaagtgct cgcttttagt ctgttcgccg
     2761 ctggcgaccg cgaggatttt ttcgaacagc tcgcggccca cgtcctcgac gctgcggccg
     2821 gccaggatct cgccggcatt gaggtccatg tcgtcaatca tccgctcgta catgggggtg
     2881 ttgctggcca ccttgatcgt cgggcacggc ttgaggccaa agcagctgcc gcggccggtg
     2941 gtgaagacca tcatgttgca gccgccggcc accaggcccg tgacgctggg cgggtcgaag
     3001 ccgggcgtat ccatgacgac catgcctttg cgggtgatcg gctcggcata ctggtacacc
     3061 tcggtcatcg cggtcgagcc ggccttggcg acggcgccga gcgatttttc ggcgatcgtg
     3121 gtcaggcccc cctccttgtt gccgacggac gggttgttgt cgatctcgac gccgtacagg
     3181 cccgtgtgcc agagccacca ctggatccgg gcgagcagct tttcggccac ggctgcggtg
     3241 accgcgcgcc gggtgagcag gtgctcggcc ccgtaaattt cggtcgtttc ggccagggcg
     3301 ctggtgccgc cgcaggcaac gatcatgtcg gaggcgatgc cgagggccgg attgcaggtg
     3361 acgccgctgt tgccgtcgga gccgccgcaa ttggtgccga gcacgatctc gctggcgggc
     3421 agcgtttcgc gccgggcggc gtttacttgc ggcaggagct gctcgacgag gcgcactccc
     3481 tcggccacgg ttttcgccgt gccgccgagg tcctgcatcg agagcagggg gggcccgctc
     3541 gccttgccat tgccgccgat ttgcatcagg ttttgcgact ggagcaggta gcccatcgct
     3601 ccctgctcgc accccaggcc gacgagcaaa tagccgccga tattcgggtg gcgagccatg
     3661 ccggccagcg tgcggttcag aagctcgtgc tggaggccct gatactgcat gccgcagccg
     3721 agggcgtgct tgatggccag gacgccatcg acgccgggaa aatcgcgcag ccgctccggc
     3781 gtgaaatgct gggcgatgta gcgggtgacg ctggccgagc aattcaccgt ggaaat
//
LOCUS       DASUGB010000707         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1051089, whole genome shotgun sequence.
ACCESSION   DASUGB010000707 DASUGB010000000
VERSION     DASUGB010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051089"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFV87_13520"
     CDS             <1..780
                     /locus_tag="VFV87_13520"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104832.1"
                     /translation="ACPSSDLCTALAAKLLPSLDIEALARLSCRASALPDERGQFTWH
                     SDRSRFRIAVANDEAYHGYYPETLELLEAAGAELCDFSPLASGAIPAGVDVVYFGCGH
                     PERHAAQLAGNHCLKQSLRCFAAAGGRIYAEGSGLAYLCREIVFPCGNTVPMSGLLPA
                     TARWLPHASEPEPTEVTFGATSWLVPSRTVLRGYRHPGWQIEPRGPMITYAAGARQRL
                     DVLGRGNVIGSQVLFNLAANRHLLRPFFEPYATVSASAHVE"
     gene            807..1133
                     /locus_tag="VFV87_13525"
     CDS             807..1133
                     /locus_tag="VFV87_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5104833.1"
                     /translation="MPQTRREKIEALLQDEPHDQFLRYGLAVELDNEGRFDESLAIFR
                     GLMQDQPPHVASYFRGAQLLVKLDKIDQARATLREGIEIARQQGDLHAAGEMGELLAN
                     IGALGS"
     gene            1222..1617
                     /locus_tag="VFV87_13530"
     CDS             1222..1617
                     /locus_tag="VFV87_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104834.1"
                     /translation="MHARYTRGWILIPGLILLALALVVHWFVTPLVTLQLPLMGRIMV
                     AAAFAGAALFPLLIFDAVVSEIVRQWLPATCPRCSGRIYYVPQPDPSERAQRQPVEYL
                     CRDCVQRGELKLRYRDVNPQFNNRSSHRR"
     gene            complement(1666..2415)
                     /locus_tag="VFV87_13535"
     CDS             complement(1666..2415)
                     /locus_tag="VFV87_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104835.1"
                     /translation="MWCSTCQQDVAPLASAEPGTSLRCAKCDRVLAAAPAPEIEPSKA
                     AVPPPRPRILDDWELEAELRGVQRLVGSLKASGCEAGALLPLRFDVPPQESVRPPSED
                     RSTQQTPAADRPKSHFFAWMLLSLGLATFACGGVLLGWSIAADRADLWPLGMPLALIG
                     QAGLIIGLVLQLDGLWQSNRKTTETLSQLDGELSRVRHATTLLTTTKNASAQSFYAHM
                     AEGASPQLLLADLKGQLDLLAQQMASQRPRK"
     gene            2738..>3085
                     /locus_tag="VFV87_13540"
     CDS             2738..>3085
                     /locus_tag="VFV87_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104836.1"
                     /translation="MYGGPSLAWVAGIVAVNCAAAALAQSPGETGREEQKIARLIKQL
                     DADRFEDRERASRELREIGQPALAALRRAAEHPSLEVRTRAKAITSSIVDGPRLREFI
                     AFASQPNERLDLEH"
BASE COUNT          526 a         1008 c          997 g          554 t
ORIGIN      
        1 gcgtgtccgt cgtccgattt gtgtaccgca ctggccgcga agctgctgcc gtcgctcgat
       61 atcgaggcgc tcgcgcgcct ctcgtgccgg gcgtcggcgt tgcccgacga gcgcggtcag
      121 tttacctggc actccgatcg cagccggttc cgcatcgccg tggcgaacga cgaggcctac
      181 cacggttact acccggagac gctggagttg ctcgaagcgg cgggcgccga gctgtgcgat
      241 ttttcgccgc tggcgagcgg ggcgattccc gcgggcgtcg atgtcgtcta tttcggctgc
      301 ggccatcccg agcggcatgc ggcacaactg gccggcaatc attgcctcaa gcagtcgctg
      361 cggtgcttcg ccgcggcggg cgggcggatt tacgcagaag gcagcgggct ggcttacctg
      421 tgccgcgaga tcgtgttccc ctgtggcaac acggtgccga tgagcggcct gttgcccgct
      481 acggcacgct ggctgccgca tgcttcggag cccgagccga ccgaggtaac cttcggggcc
      541 acatcgtggc tcgttcccag ccgcacggtg ctccgcggct atcgtcatcc gggctggcag
      601 atcgagccgc gcgggccgat gattacctac gccgcgggcg cccgccagcg actggacgtg
      661 ctcggccgag gcaacgtgat cggcagccag gtgctgttca atctggccgc caaccggcac
      721 ctcttgcggc cttttttcga gccttatgcg accgtgtctg cgtcggctca tgtcgagtga
      781 cgcaatcggt atttctgact atcttcatgc cgcaaacccg tcgcgagaag attgaggccc
      841 tgctacagga tgagccgcac gaccagtttc tgcgctacgg tctggcagta gaactggaca
      901 atgaggggcg gttcgacgag tcgctcgcaa tttttcgcgg cttgatgcaa gaccagccgc
      961 cacacgtcgc ctcgtacttt cgcggggcgc aactgctcgt caagctcgac aagatcgacc
     1021 aggcccgggc gacgctccgc gaagggatcg aaatcgcccg ccagcaaggc gacttgcacg
     1081 ccgctggcga aatgggggag ctgttggcga acatcggcgc cctgggttcc tagtgcagca
     1141 cgcaagggcc gctgccaact tataggtggc gcgatccgcc ggaaggttgc caattgcgtt
     1201 gtcgcgaaca tgacgcctga aatgcatgcc cgctacacgc gcggctggat tctcatccca
     1261 ggactaatct tgttagctct cgcgctggtc gttcattggt tcgtcacgcc actcgtcaca
     1321 ctacaactgc cactgatggg ccgaatcatg gtggcagccg catttgccgg cgcagcgttg
     1381 tttccattgc tgattttcga cgcagtcgta agcgaaatcg tgcgtcaatg gctgccggcc
     1441 acatgtccgc gatgcagcgg gcggatatat tacgttccgc aacccgatcc gagcgagcga
     1501 gcgcaacggc agcctgtgga gtatctatgc cgggactgcg tgcaacgtgg cgagctgaag
     1561 ctgcgctacc gtgatgtcaa ccctcaattc aataaccgga gcagccatcg tcgctaaggc
     1621 tcgagggttg gtctgaaatc cccgtggagc acgcatcgcg tatggctact tgcgcgggcg
     1681 ttgcgaggcc atctgctgcg ccagcagatc gagctggccc ttcagatcgg ccaacagaag
     1741 ctgcgggctg gcgccttcgg ccatgtgggc atagaacgac tgggcgctgg cattcttggt
     1801 ggtcgtgagg agcgtggtgg catgccgcac gcgcgacagc tcgccgtcga gttggctgag
     1861 cgtttccgtc gtcttgcgat tgctctgcca caggccgtca agctgcagaa ccaggccgat
     1921 gatcagcccg gcctgcccga tcaaagccag cggcattccc agcggccaca ggtcggcacg
     1981 gtccgccgcg atcgaccagc cgagcagcac gccgccgcag gcgaacgtcg ccaggcccag
     2041 cgaaaggagc atccaggcaa aaaagtggct cttggggcga tcggctgcgg gcgtctgctg
     2101 cgtgctgcga tcttcagacg gcggccgcac cgattcctgc ggcggaacat caaaccgcag
     2161 cggcaacagc gcccccgcct cgcagccgct cgccttgagc gaccctacca gccgctggac
     2221 gccgcgcagc tccgcttcca gctcccagtc gtcgaggatc cgcggccttg gaggcggcac
     2281 cgcggctttg ctcggctcga tttcaggtgc cggcgcggcc gccagcacgc gatcgcactt
     2341 tgcgcagcgc agcgacgtcc ccggctcggc tgatgcgagc ggggcgacgt cttgctggca
     2401 ggtcgagcac cacatcggct gaaattcgaa gaaccaaaat cgaagccaag ccgctagcag
     2461 agtccgcgcg ctggaagcgc gggcctcaca ggtcgaaatc ggcggcctgc gcgcgaaact
     2521 ataccgattt tgccgattgt gccggttttg ccgcctggcg aggttgcttg cacccctgcg
     2581 aatcaaccaa cacggggtcg ggggtccatt gcgatcacga ctctccatct gccagaacag
     2641 ctaccgcaat tgactcccga ccccttccag cccgccgggc gagtttgctt gcgtggctgc
     2701 ggcctgccct cgtatgctga gcggacgagg aacaagcatg tacggcggtc cttctctcgc
     2761 ttgggtggcc ggtatcgtgg ccgttaactg cgccgcggct gctctcgccc aatcgccggg
     2821 cgaaaccggc cgggaagagc agaaaatcgc ccggctcatc aagcagctcg atgcggaccg
     2881 gttcgaggac cgcgagcggg catcgcgaga gctccgagaa atcggccagc cggcgctggc
     2941 tgccctgcgg cgagccgcgg agcatcccag cctggaagtc cgcacgcggg ccaaagcaat
     3001 cacgagttcc atcgtcgatg gcccgcggct gcgggagttc atcgccttcg cctcgcagcc
     3061 caacgagcgg ctggacctgg agcac
//
LOCUS       DASUGB010000708         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_496256, whole genome shotgun sequence.
ACCESSION   DASUGB010000708 DASUGB010000000
VERSION     DASUGB010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496256"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV87_13545"
     CDS             complement(<1..188)
                     /locus_tag="VFV87_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5104837.1"
                     /translation="MRRVCALGMGAAADRSVLRARVSFWCPCSLGEGRGMASKSVHRR
                     GFTLVELLVVIAIIGILVA"
     gene            complement(262..1464)
                     /locus_tag="VFV87_13550"
     CDS             complement(262..1464)
                     /locus_tag="VFV87_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5104838.1"
                     /translation="MEYRFSEGAEQSRPSAAAQLPRSLTTLLPLLVLLVIVLLIVQFW
                     TMSRGGGRPIVQFDPTAKPRPIAPAGELAADEQATIDLFKQTSRSVVHVTTAQVGRDL
                     AFNVMEIEKGTGSGFMWDEQGHVVTNYHVVEEANRFRVTLADQSTHEASLVGTAPDKD
                     LAVLKIDAPVELLQPLLIGESKDLEVGQKVFAIGNPFGLDQTLTTGVISGVGRQIRSL
                     TGRVIEGVIQTDAAINPGNSGGPLLDSRGRLIGVNTAIYSPSGASAGIGFAIPVDTVQ
                     RIVPQIVRTGSVVRPSIGAQFFPDSFTRRLGVEGVLIGRVSPGGPADEVGLLPTRRDE
                     WGDISWGDLIVAVDGRAVESVDELLTVLENHNVGDSVKLKIRRGLRSTAEKALEVTIR
                     LEAEADSS"
     gene            1624..>2004
                     /locus_tag="VFV87_13555"
     CDS             1624..>2004
                     /locus_tag="VFV87_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104839.1"
                     /translation="MNRVRTALFLLVAGLFVFRPSMAGAQDAPPRVIAFERGYAQGGD
                     SVAAGQLLLGELNCTSCHAADALLAVAIQKKQAPVLDSVCSRVKPQYLLKFLADPLAA
                     KPGTTMPHVLAGVPEAERGPIAEAL"
BASE COUNT          386 a          675 c          586 g          357 t
ORIGIN      
        1 gcgaccagaa tgccaatgat ggcgatgacg acgagcaatt cgacgagagt aaaaccacgc
       61 ctgtgaactg atttagacgc cataccgcga ccctccccta aggaacacgg acaccagaac
      121 gaaacgcgag cgcgcaacac actgcgatcg gcggccgcgc ccatcccaag cgcacaaacg
      181 cggcgcatcc ccacaccaca tgcaactggg aacccagtcg cccctgaatc tagtcttggc
      241 gcgagaagaa agtcaactat ttcacgacga atccgcttct gcctccagcc gaatcgtaac
      301 ttccagcgcc ttttcggcag ttgaccgcaa tcctcgccgg attttcaatt tcacggaatc
      361 acccacattg tgattctcca agaccgtcaa cagctcatcg acgctctcca ccgctcggcc
      421 gtccaccgcc acgatcaggt ctccccagga aatgtcgccc cattcgtccc gacgcgtcgg
      481 caggaggccg acttcatcgg ccggaccgcc cggtgaaacg cgcccgatca gcaccccttc
      541 gacgcccagg cggcgcgtga acgaatcggg aaaaaactgc gcgccgatcg agggccgcac
      601 cacgctgccc gtgcgcacga tctgcggcac gattcgttgg accgtatcca cgggaatggc
      661 aaagccgatg cctgccgagg ctcccgaggg gctatagatc gcggtgttga cgccgatcag
      721 ccgtccgcga ctgtcgagca agggaccgcc ggagttgccc gggttgatgg cggcgtcggt
      781 ctggatcacc ccctcgatga cgcgcccggt gagcgagcgg atctgccggc cgacgccgct
      841 gatcacgccg gtggtcagcg tctgatcgag gccaaacggg ttgccgatgg cgaacacctt
      901 ttggccgact tccaagtcct tcgattcccc gatgagcaac ggctggagca actccaccgg
      961 ggcgtcgatc ttgagcacgg ccaggtcctt atccggcgcc gtgccgacaa gcgacgcctc
     1021 gtgcgtgctc tgatcggcga gcgtcacacg aaagcgattt gcctcctcga cgacgtggta
     1081 gttcgtcacg acgtgcccct gctcatccca catgaaaccg ctgccggtcc ctttttcgat
     1141 ctccatgaca ttgaacgcca ggtcgcggcc aacctgggcc gtcgtgacgt ggacaacgga
     1201 gcgagaggtc tgcttgaaca ggtcgatggt cgcctgctcg tcggctgcca actcgcccgc
     1261 gggagcgatt ggccgcggct tggcggtggg atcgaactgc acgatcggcc ggcccccgcc
     1321 ccggctcatc gtccaaaact gcacgatcag cagcacgatg accagcagca ccaagagggg
     1381 cagcaaagtc gttaacgacc gcggaagttg cgccgcggcg gatggacgag actgttcggc
     1441 gccttcggag aatcgatatt ccatgatgcg acctcagcgt aattagtttc ctgccagccg
     1501 aaacctgaga atagcgcgcc gtccctcgcc aggcgagagg ttgacatcca ccagcccgac
     1561 accgcataaa tgagcttccc cttactgttt cccgcctgat cgcccgccgc tggagcgagc
     1621 ttcatgaatc gcgttcgtac cgctctcttc cttttggttg cgggcttgtt cgtttttcga
     1681 ccttcgatgg caggcgctca ggatgccccg ccgcgcgtca ttgcgttcga gcgtggatat
     1741 gctcagggcg gtgactccgt cgccgccggc cagttgctcc tgggcgagct gaactgcacg
     1801 agctgccacg cagcagatgc gttgcttgcg gtggcgatcc aaaagaagca ggctccggtg
     1861 ctcgacagcg tctgctcccg cgtcaagccg cagtatctgc tcaagttcct cgccgatccg
     1921 ctggctgcca agccgggcac gacgatgccg cacgttttgg ccggcgttcc cgaggccgag
     1981 cgcgggccga ttgccgaggc actg
//
LOCUS       DASUGB010000709         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2024317, whole genome shotgun sequence.
ACCESSION   DASUGB010000709 DASUGB010000000
VERSION     DASUGB010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024317"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFV87_13560"
     CDS             complement(<1..654)
                     /locus_tag="VFV87_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104840.1"
                     /translation="MSRLTGRNFVVNVKHLPTTLASYGKCQDAATTVVANRQHVQCQT
                     NRTAVVEILLKQESILPEAVEAFYSASPLAGDNPVETWIWHAAVMSDQERSIVGGQHY
                     TIPHFEFSDCRFVDLPPAVIVAESHVYCFGSHPFARQLAGLLDPGGELGFVEGGVLVD
                     VEVADSLVLGFAGGGMGRSDVPSYRPQVNDEREVFLDVLEKLVIRRDELAPLPFCQGD
                     "
     gene            748..1164
                     /locus_tag="VFV87_13565"
     CDS             748..1164
                     /locus_tag="VFV87_13565"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5104841.1"
                     /translation="MKKTTTTAKLTDWRGELLARIRKLIQQADPEAVEEIKWRKPSNP
                     AGVPVWSHGGIICTGETYKDKVKLTFAKGASLPDPAGLFNASLEAGTRRAIDIHEGDK
                     IDEKAFKALIRAAVALNTSAPTAARPVRSRKKPNRA"
     gene            complement(1564..2163)
                     /locus_tag="VFV87_13570"
     CDS             complement(1564..2163)
                     /locus_tag="VFV87_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104842.1"
                     /translation="MTLALFNLSNSPTMFLSIHDLVQFTAGQLRLASMPPLEGAWVQV
                     PRIVLDSRLVERGDLFWRVEPQECDTQLAYLRGAIGIVTDGRQVEPWPGTFSLHVEGA
                     IDSLERLIDALQTGQMAEIPERFLLDEPIAESLAGTDEAEELSVDILPLKRAAESSED
                     FSELKVLQLSARGGIANFPLTCGQSAKGQLAARCRRRAA"
BASE COUNT          481 a          688 c          708 g          402 t
ORIGIN      
        1 atcgccctgg cagaacggga ggggtgcgag ctcgtcacgg cggatgacaa gcttctcaag
       61 aacgtccaga aagacttccc tttcgtcatt cacctgggga cggtatgagg gcacgtcact
      121 gcgtcccatc cctcccccgg cgaatccaag caccagggag tccgctacct cgacatctac
      181 gaggacgccc ccttcgacga agcccaattc accgcctggg tccagcaagc cagccaactg
      241 ccgggcgaac ggatgtgagc cgaaacaata cacatgagat tccgcaacga tcactgctgg
      301 gggcagatcg acgaagcgac agtcactgaa ttcgaaatgc ggaatggtat agtgctgccc
      361 gccgactatc gagcgttctt gatcgctcat cacggcggcg tgccagatcc aagtttctac
      421 tggattgtcc ccggcgagtg gggaagcgga atagaaagcc tctacggctt cgggaaggat
      481 ggattcttgc ttcagcagta tttcgacaac cgcggttcga ttggtttgac attggacatg
      541 ctggcgattg gcgacgacgg ttgtggcggc atcctggcac ttgccatagc tggcaagtgt
      601 tgtgggcagg tgttttacgt tgaccacgaa attgcgcccg gtgaggcggg acatgagcgc
      661 ttactggcat cgtcgttcac tgaattccta gaaggccttt gtgttgcccc ggagtactga
      721 accgggccga gtacgcgtga caaagacatg aagaaaacga caaccacagc gaaactcact
      781 gattggcggg gcgagttgct cgcgcgaatc cgcaagctca tccagcaggc cgatcccgag
      841 gcggtcgagg agatcaaatg gcgcaagccg tcgaacccgg ctggggtgcc ggtctggtcg
      901 cacggcggga tcatttgcac gggggagacg tacaaggaca aggtgaagct gacgttcgcc
      961 aagggggctt cgttgccgga cccggcgggc ctgttcaacg ccagcctgga agcgggcacc
     1021 cggcgggcga tcgacatcca cgagggggac aagatcgatg aaaaggcatt caaggcgctg
     1081 attcgcgccg ccgtggcgct caacacatcg gcaccaaccg ctgctcgccc cgtgcgctcg
     1141 cgaaagaaac caaatcgcgc ttagagccaa gcgccctcat tccggccctc tctcagagcc
     1201 agaggcggca atgcgcctgc ggccaacaag aaacgaccgc gggcgctgcg accgaccgat
     1261 cgcagcgccc gcgtggtgtt ccctgccgcc ggtgcggttt ttgaagccgc accagcgaca
     1321 gtaaaaaagc ttcaccggga agcacccggc cgtgcaattg acccagaaac tgcacggtca
     1381 ggcttaattg cttcaaccca ggagggaggg cggcgactgg ttcccgcaaa ccggccgcca
     1441 gccgatgaag cttcctcaaa tggcttgtga gtgggtgagt ggtgagtagg tgatttgcgt
     1501 tagcgcgcaa tgggtgcgag gactcatgac gcgtgagaac ccgctggcaa cggaatcggg
     1561 cggttacgcc gctcgtcgcc ggcacctggc ggccagttgg cctttggccg attggccaca
     1621 ggtgagcggg aaattagcaa tgcccccgcg ggccgacaat tgcaatactt tcaattctga
     1681 aaaatcttcc gaactttccg ccgcccgctt cagcggcagg atgtccacgg acagctcttc
     1741 ggcctcatcg gtgccagcca gggattccgc gatgggctcg tccagcagaa atcgctcggg
     1801 aatttccgcc atctggcccg tctgcaaagc atcgatcaag cgctccagcg agtcgatcgc
     1861 gccctccacg tgcaggctga acgtccctgg ccagggttcg acctgccggc cgtcggtgac
     1921 gatgccgatc gccccgcgga gataggccaa ctgcgtatcg cactcctgcg gctcgacgcg
     1981 ccagaacagg tcgcctcgct cgaccagccg cgaatcgagc acgatccgcg gcacctgcac
     2041 ccacgccccc tccagcggcg gcatcgacgc cagccgcaat tgaccggcgg tgaactgcac
     2101 gagatcgtgg atgctgagga acatggtcgg gctgttggag agattgaaga gggccagagt
     2161 caaacgcgtg gttgacgaga aggggtcggg agtcaattgc ggtagccgcc tttccattcg
     2221 tagtgccatg accgcaattg actcccgacc cccttcgtgt ggctacccga cgatgcgaa
//
LOCUS       DASUGB010000710         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_496750, whole genome shotgun sequence.
ACCESSION   DASUGB010000710 DASUGB010000000
VERSION     DASUGB010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496750"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFV87_13575"
     CDS             complement(<1..695)
                     /locus_tag="VFV87_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104843.1"
                     /translation="MHVRRTGTHVEVLTPSKLNLFLEVLARRPDGFHEIQTLIAPVSI
                     YDTLTFQPRGEPGIKFDCRWAHGLTARGLRECAGENEGAFGDLPSGPDNLAWKAVELL
                     RGRAIEKRGARLTLVKRIPAAAGLGGASSDAAAALVAANLGWNLGWKRERLAELAAEL
                     GSDVPFFLTRGAALCRGRGEAIEPLRAAPLHVTVVRPPVGLATGDVYRCCRPATQPVS
                     AQPLREALECGKAA"
     gene            complement(1026..2291)
                     /locus_tag="VFV87_13580"
     CDS             complement(1026..2291)
                     /locus_tag="VFV87_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5104844.1"
                     /translation="MICLGCLSATAEDWPRWRGPRGDGTWNEPGIVEKFPSATIARKW
                     TAEIGAGYTSPTVAEGRVYLMDRLTKPTSTERILCFDEETGRELWKVAYECEYGKIGY
                     QAGPRAAVTIDGGKAYALGATGRMHCLNAATGEIVWQRDLERDYDIQMPIWGLAGSPL
                     IVQDLVVLHIGGRGGACIVALDKNSGKEAWKALDDRGQYTTPVLVEQAKTPVLICWTG
                     DSVAGLEASTGRVLWRHPWKPRNMPIGIATPVVEKDRVFFTSFYDGSLMLRLAADKPE
                     AEVLWKLVGTSERITDALHSIISTPVFDGGHIYGVDSYGELRCLDADNGERLWEDLTA
                     TPQSRWSTIHFVKNGERYWLFNERGELIIGKLSPRGFEEISRSKLLDPTTEQLRQRGG
                     VCWSHPAFANQCVFARNDKELVCASLAEP"
     gene            2432..3391
                     /locus_tag="VFV87_13585"
     CDS             2432..3391
                     /locus_tag="VFV87_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5104845.1"
                     /translation="MAAHAIPRTGHSQEKAAVAADAIPIIDCHQHLWDLEGFKLPWIE
                     PGTLLGRSYVMQDYLAAIEGTGIKHAVYMEVDVDPAQQTAEAEHLIEICKSGKAPTIA
                     AVISGRPNSDGFKDYITPLAKSPYIKGVRQVLHGGSTPAGYCLEKPFVRGIQLLGELG
                     ESFDLCMRAKELADGAKLAAQCPDTRFIVDHCGNADVKAFFKHGDKRLEGVTPDHSAD
                     QWKRDIDTLAAKKNVICKISGIIARVPKQWSADDLAPVVSHCLDSFGPQRVIFGSDWP
                     VCLLGAPLVQWVSALRDIIASRPAEQQKRLLMSNAVAFYGLKL"
     gene            complement(3373..>3773)
                     /locus_tag="VFV87_13590"
     CDS             complement(3373..>3773)
                     /locus_tag="VFV87_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX5104846.1"
                     /translation="PLLSINRSCQTCHRYPEEELLARAEAIQDRTQALMDRAEIACVD
                     LIETIERQAAAEPMSESLAKARRLHRQAQFRLDFVAAENSMGFHAPQEAARILGEAID
                     LARQGQIAVLERSANSGAHEQASGDQSFRP"
BASE COUNT          658 a         1318 c         1131 g          666 t
ORIGIN      
        1 gctgccttcc cacattctaa cgcttcgcgc agcggctgtg cgctcacggg ctgggttgcc
       61 ggccggcagc agcgatagac gtcgcccgtc gccaacccca ccggcggccg gacgaccgtg
      121 acgtgcagcg gcgccgctcg cagcggctcg atcgcttcgc cccgcccgcg gcacagggcc
      181 gcgccgcgcg tcaggaaaaa cggcacgtcg ctcccgagct ccgcggccaa ttccgccagc
      241 ctctcgcgct tccagccgag gttccagccg agattcgccg ccaccagcgc cgccgccgca
      301 tcgctcgaag ccccgcccaa tccggcggcg gctggaatcc gtttaacgag cgtcagccgc
      361 gcgccccgtt tctctatcgc tcgcccgcgc aacagctcca ccgccttcca cgccagattg
      421 tccggtccgc tcggcaaatc gccaaacgcg ccctcgttct cgcctgcgca ttcccgcaaa
      481 ccgcgcgccg tcaatccatg tgcccaccgg caatcgaatt tgattcccgg ctcgccccgc
      541 ggctgaaacg tcagcgtgtc gtaaatcgaa acaggcgcga tcagcgtctg gatctcgtga
      601 aatccgtcgg gccgccgagc gagcacctcc aagaacaagt tgagcttgga gggcgtcagg
      661 acttcgacat gcgtgccagt tcgtcggacg tgcatccggt gcttcgccag ccacgaggat
      721 cggcgtcaac ttttcccctc gcccttgtgg gagaggggcg ggggtgaggg accgccattg
      781 cacgagagta gctacacaag atgaatccgt ctggcggttc tcagcacccg tgattgtact
      841 gcccgccccg cgcgggtcaa tctgaatcgt cgcaggcttc tcggcatcgc aggcttctcg
      901 cgccgcgaga agcaatggac tctccgggcg gaaaggcgcc gcgcttctct cacgtcccat
      961 cgagccagcc cagcgcgacg ttgaagcgac caccgacgtc gattgcgact cgcggagcga
     1021 gtcgcctacg gttccgccaa acttgcgcac accagctcct tatcgttccg tgcaaagacg
     1081 cactggttcg caaacgccgg atgcgaccag cacacgccgc cgcgctgccg caattgctcc
     1141 gtcgtcggat cgagcagctt cgagcggctg atttcttcaa agccgcgggg cgacagcttg
     1201 ccgatgatca actcgccgcg ctcgttgaac agccaatacc gctcgccgtt cttgacaaag
     1261 tggatggtgc tccaccgcga ttgcggcgtc gcggtcaagt cctcccacag ccgctcgcca
     1321 ttgtccgcat cgaggcaccg cagctcgcca tagctatcga ctccgtagat gtgcccgccg
     1381 tcaaacaccg gcgtgctgat gatcgagtgc agggcgtcgg tgatccgctc gctggtgccg
     1441 acgagcttcc acaggacttc cgcctccggc ttgtcggccg ccaggcggag catcagcgag
     1501 ccgtcgtaaa acgacgtgaa aaacacgcga tccttctcga cgaccggcgt ggcgatgccg
     1561 atcggcatgt tccgcggctt ccaggggtgc cgccagagga ccctgccggt cgaggcttcc
     1621 aaaccggcga cgctgtcgcc ggtccagcaa atcagcacgg gcgtttttgc ttgctcgacc
     1681 aagaccggcg ttgtgtactg gccgcggtca tccagcgcct tccatgcctc tttgccgcta
     1741 ttcttgtcga gcgcgacgat gcacgcgccg ccgcggccgc cgatatgcag cacgaccaga
     1801 tcctgaacga tcagcggcga gccggcgagc ccccagatcg gcatctggat gtcgtagtcg
     1861 cgttccagat cgcgctgcca gacgatttcg cccgtcgcgg cattcaggca gtgcatccgg
     1921 ccagtggcgc cgagggcgta cgccttgccg ccgtcgatcg tcaccgccgc tcgcggcccc
     1981 gcctgataac cgatcttgcc gtactcgcat tcgtaggcca ccttccacag ctcgcggccg
     2041 gtctcttcgt caaagcacaa gattcgctcg gtagatgtcg gcttcgtgag gcggtccatc
     2101 agatagacgc ggccctcggc gaccgtcggg ctcgtgtatc ccgcgccgat ctcggcggtc
     2161 cacttccgcg cgatcgtcgc tgacggaaac ttctcgacaa ttcccggctc gttccacgtt
     2221 ccatccccgc gcggcccgcg ccatcgaggc cagtcctccg cagtcgcaga caggcaaccg
     2281 aggcaaatca tggcgaacac aatcgagcgg gcgtgtatca tagggttggt tcctgttcca
     2341 atctcttctc tttggccagt tctgaggaca ttgtacgacc atggaccgac gccaattctt
     2401 agctgccgct gccgccgccg caactgcaga gatggcagca cacgcaattc cgcgaaccgg
     2461 acactcccag gagaaagccg cggtggctgc tgacgcgatt ccgatcatcg actgccatca
     2521 gcacctctgg gatctggaag ggttcaagct cccctggatc gagccgggaa cgctcctggg
     2581 ccgcagctac gtcatgcagg actaccttgc ggccatcgag ggcacgggaa tcaagcacgc
     2641 cgtctatatg gaggtcgatg tcgatccggc ccagcaaaca gccgaggcgg agcatttgat
     2701 cgagatctgc aagtcgggaa aggcacccac catcgccgcg gtcatctccg gacggccgaa
     2761 ctcggacgga tttaaggact acatcacgcc gctcgccaaa agcccctaca tcaaaggcgt
     2821 gcggcaagtc ctccacggcg gcagcacgcc cgccggctat tgcctggaga agccgttcgt
     2881 ccgcggcatc cagctcctcg gcgaactggg cgagagcttc gacctctgca tgcgcgcgaa
     2941 ggaactcgcc gacggagcga agctcgccgc gcaatgcccg gatacgcggt tcatcgtcga
     3001 tcactgcggc aacgccgacg tcaaggcgtt tttcaagcac ggcgacaagc gcctcgaggg
     3061 cgtgacgccc gaccattccg cggaccagtg gaagcgcgac atcgacaccc tcgcggcgaa
     3121 gaaaaacgtc atctgcaaaa tcagcggcat catcgcccgc gtccccaaac agtggtcggc
     3181 cgatgatctc gcccccgtcg tcagccattg cctcgatagc ttcggccccc agcgcgtcat
     3241 cttcggcagc gactggcccg tctgcctcct cggtgcaccg ctggtgcagt gggtcagcgc
     3301 cctccgtgat atcatcgcgt cgcggccggc cgaacagcag aagagactgc taatgagcaa
     3361 tgcagtcgcg ttctacggcc tgaagctctg atcgccgctg gcctgttcgt gagcgcctga
     3421 gtttgcactc ctttccagca ccgcgatctg cccctgccgg gcgaggtcga tggcttcgcc
     3481 caggatgcgg gcggcttctt gcggggcgtg aaaacccatc gagttctcgg ccgcgacgaa
     3541 gtccagccgg aactgggcct ggcggtgcag ccggcgggcc tttgcgagcg attcgctcat
     3601 cggttcggct gcggcttgcc gttcgatcgt ttcgatcaga tcgacgcagg cgatttccgc
     3661 gcggtccatc agcgcctgcg tgcggtcctg gatcgcctcg gcccgcgcaa gcagctcctc
     3721 ctcgggatag cggtggcagg tctggcacga gcggttgatc gagaggagcg ggc
//
LOCUS       DASUGB010000711        15087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1190419, whole genome shotgun sequence.
ACCESSION   DASUGB010000711 DASUGB010000000
VERSION     DASUGB010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190419"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2532
                     /locus_tag="VFV87_13595"
     CDS             <1..2532
                     /locus_tag="VFV87_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104847.1"
                     /translation="GDQGAVETAAALHQLTQMGTMIGTPDYISPEQAGDARKADIRAD
                     IYSLGCTLYTLLAGRAPFGEGSVLEKIKAHSEQEARPLADFRNDVPAEVEAIVRKMLA
                     KDPADRFQTPSEVAAALEKWATNQPLSATANQARARRRALALTALATIISAIAAGVLI
                     YLATDTGRLEINSDVEIPGAKIVLTKNGQEYASFDVEPGAKQQTIRAGDYEVSLSGAP
                     PGVDLDVRSTKRGKPRDYGAPYSPKDKIIAVYRGGGMSIEIAKQLTPATAVPIQDPNA
                     PSEFFQVLGELQAIVRKALIEEGLTVGQFNPGTQVAGPRRLIVLQAEATGERYDQNKV
                     LTALRNEFKALATQNDAFVSSLDHQMHGGVAFTNLPYTTPAREGLIRVTMRSLGTDKR
                     EITIAKWRLVIQVEEWPLTKGNIAAPDGQSDSPSQYFERLAGEPLESAVRERVGGAVG
                     RRITSERIPQPPGKLRYEAQIDGVGSKIIDQVFVDLRDVLMRGTGARIVHEGESGIDL
                     RLLRYTTPTHNGEVRVTLISRELGGQPADESTKWLLKIDASEWAKGTSQASPSVVRVE
                     GSGEQMILSLDGAPLTRAELLLRLDALATGDPGFLVYVEGDVVPVTKDVTDLLKDIGA
                     TAVGEKNVRFPSEVTGQIMDDAVRRGKAEAAERNQPPTASSASLTEGQIKELVSEAAG
                     IPNDDWTKLTSGMEVPSPNTLQSQTLSLMLFSLRALPPAGIESELPAIANLKKDFQYL
                     PEQTPKPAEIARALWISKSKGYASYIQPEYVTEVTVQVAGDTASGEVAFEKEGLYRGR
                     VRYVARRADGDWRIEEFYLPNVGLSLVRNEQGVWERMIQVQESPN"
     gene            2538..2774
                     /locus_tag="VFV87_13600"
     CDS             2538..2774
                     /locus_tag="VFV87_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104848.1"
                     /translation="MSESRCRRTIYVSVLALSAGAALTGETVSAARLLKAEIEVDGKL
                     VLQTAYSDDGTEQVATVWRYLARHLVAAHSIRGC"
     gene            complement(2866..3975)
                     /locus_tag="VFV87_13605"
     CDS             complement(2866..3975)
                     /locus_tag="VFV87_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5104849.1"
                     /translation="MSSTSPVPNQPVAGLESQATGQGASVDVASEPTPPEAASANPAE
                     TADVPEVDEQPPARYYRRRVGLPIALFVLTCGSTFLAGATSYVPHEYMFRALDFGITN
                     TGGVPTEPSLMPLRRAILANWREGLLYMGCVLAILLTHEMGHFVMTLVYRVRASLPFF
                     VPLPVSPIGTMGAVIAMDSRIANRRQIFDIGLAGPLAGLVVALPIMWIGVQHMDLTPV
                     PPSGDNLQVPLAMRLAIEYLHPGKYDPGTGFALTRANAWFMAGWVGLLVTGLNMLPVS
                     QLDGGHVAYALLGRKAHWLARGFMAVACLYVMVMFFGFGVPPAWVLMIALVLFIGTDH
                     PPTRDDSVRLGWLRTTLGYASLLIPIFCFVPTLQF"
     gene            complement(4010..5410)
                     /locus_tag="VFV87_13610"
     CDS             complement(4010..5410)
                     /locus_tag="VFV87_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5104850.1"
                     /translation="MPSLFETSEADNLRQAQPLAARMRPQSLGDFVGQQRFLGTGKLL
                     QRLLAADRLSSVIFYGPPGTGKTTLARLLATESRKRFKQLSAVTSGVKELREVLEAAR
                     DDLAGSGRRTLLFIDEMHRFNKAQQDALLPDVEEGIVTLVGATTSNPFFAVNSALVSR
                     SQIFEFQPLSPEEIKTIIRRALADKTRGLGIYDVHLHDDALEFLAQVSDGDARRALSA
                     LEVGVLSSLSVGNAVAGVPERQGRHSQPSEGTSAPIDFNRELAAESVQRKAGAYDATG
                     DEHYDHASALIKSIRGSDPDAGLYWLARMLEAGEDVRFLCRRLIILASEDVGNADPQA
                     LPLAVAAMQACEFVGLPECQLPLAQAVTYLACAPKSNASTLAIGEARRDVRDGRILPV
                     PVHLRDSHYAGAKRLGHGEGYEYAHEADGGVAAQDYLGVQREYYHPVSRGFEVELAER
                     LKAIRARLRSADDGVE"
     gene            complement(5543..6766)
                     /locus_tag="VFV87_13615"
     CDS             complement(5543..6766)
                     /locus_tag="VFV87_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5104851.1"
                     /translation="MSNVLRLAIVDPSDNSRTQLKNMLLGMDIVWLEAECSRYEFFAD
                     VVGQTKPDIGIITIDANPEKGLKLIESVRDIAPECSVLVVSSSTDGQVILKAMRAGAK
                     EFLTLPVKVEDLISALDRISSAKFGAGDGRSRTCHVIAVCGATGGVGSTSLAVNLGCL
                     LASEQRNSAVLVDLDLALGDADVFLDAIPDYTLVDVAQNISRLDFTLLKRSLTKHASG
                     LYLLPRPVQLQDGSLITAEDLTRVLGLLKASFTHMILDVSKSFSPIDVVALKSAKDIL
                     LVTQLDLPCLRNVVRLMMSFNEVEGLKDKVKIVVNRVGLDAGVISLKKAQETMGREIF
                     WQLPNDYRTMVEVRNNGVPLIEQAPRASITQAISALAENLTSDNRKSDTPADQAAAKA
                     ATGSWLAFLGGKGKK"
     gene            complement(6846..8486)
                     /locus_tag="VFV87_13620"
     CDS             complement(6846..8486)
                     /locus_tag="VFV87_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX5104852.1"
                     /translation="MSIVSYKASLALLIAFGIILATAGRLSAQAQPGGPGVTFQVRGA
                     AERLEMTVNSSRVVEFPFDVPRMLVNNPDLVRVIPISPRSIQLSAVRAGVTQLNVWDV
                     EGNVTSIDLQILGDVQELDGTLKTLFPEASLRLRQLNSSLYISGFVPKAEMVNSIVRV
                     AQDYFPQVINDMTVGGVHKVLLHVKVMEVSRTKLRSLGFDWAHISSGGFITQNVSGLL
                     QSPGTLLGPGATVRFGIIGDGGQFYGFLEALRQYDLAKLMAEPTLTTLSGRPASFNVG
                     GEVPIPIQQALGVTTVQYREFGTRIDFVPVVLGNGLVRLEVRPSVTEVDPSLRDQVTG
                     IPGFRTRTADTAVEMKAGQTLAIAGLVYDRIEATNRGLPWLADMPWAGAAFRRVSERK
                     NEVELLIFVTPEFCDAMDPGEVPPCGPGELTTSPTDCELYFRGYMEVPKGGGPGHGGY
                     DGPMYEELPPGQAIPQAPPPPVPAPSSAQRKPAPVIKSTAANSRRPTAATRTVGTGSA
                     TASPQNGYSSYQKSGQSQKPSTAAQPTLIGPLGYDDLR"
     gene            complement(8562..9611)
                     /gene="cpaB"
                     /locus_tag="VFV87_13625"
     CDS             complement(8562..9611)
                     /gene="cpaB"
                     /locus_tag="VFV87_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX5104853.1"
                     /translation="MKSLILIFIALGCGLVASIGISQVMERGGSSGPGMEMEQILVAL
                     ADIDNNKKLDATNVRLEDWPKAKVPEGAIRRLDDAKDKFTATRFYKGEPILISKLADQ
                     ASGVAQYIPEGYRAEPVKVEEDTVMKAISPGDRVDVSVFLKKDGREIPVTGVFPILRN
                     VQIFAVNSNTERTVDAKGGEAAFRTVSLLVKEKQIAELQLAKQMGRIQLSLRRPNEPD
                     DGLGEEVLKMSELMAGRSQLGGDPTPAPAPQQSAGLSQFLSSGAQGSSSGSGSEWKLD
                     IMTPGGIEQYNWAKRDGLPQKSGAFAGDTSTLVPTPTSAPAQSAPSQSNTPADGSAES
                     TTERPSDNGSALSLE"
     gene            complement(9708..10226)
                     /locus_tag="VFV87_13630"
     CDS             complement(9708..10226)
                     /locus_tag="VFV87_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX5104854.1"
                     /translation="MIHRLYQTAIANLLSIGRQSGPVGGILAAASHKLEKPCRSYRRK
                     RRAAAAVEFAIVAPVFFLLIFGMIEYGRMVMIQQVITNASREGARAAVLDGTDVAEVQ
                     NTVNGYLATGSVSGATVTVAPDPLSDAEFGDPVTVTVSIGFDQVSWLPSPMYLGGKTL
                     TASTVMRRETVQ"
     gene            complement(10223..10789)
                     /locus_tag="VFV87_13635"
     CDS             complement(10223..10789)
                     /locus_tag="VFV87_13635"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5104855.1"
                     /translation="MELMSAYASAIAEHWTVWLVTIVLIVAAVIDGFELKVPNWVTFP
                     FILSGWVYSTVAFGWEGLGWSLLGTVVGLALLLPAYAIGGMGAGDVKLLAGVGAWVYG
                     IHTAYAFAATAVAGALLAVVMVLIQRGWKKHSAQFMVILNEIMVIRNPEQLSAIAARR
                     KPSMMLLPYGIPIAIGTIGYFLWMGMLV"
     gene            complement(11022..11207)
                     /locus_tag="VFV87_13640"
     CDS             complement(11022..11207)
                     /locus_tag="VFV87_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5104856.1"
                     /translation="MNKLLCNVRSFLVNEDGPTAVEYAVMLALIVVVCLVSIQAIGTN
                     ANATFDKIATSLPAGSS"
     gene            complement(11387..11572)
                     /locus_tag="VFV87_13645"
     CDS             complement(11387..11572)
                     /locus_tag="VFV87_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5104857.1"
                     /translation="MKKLTLRVQRFLASEDGPTAVEYAVMLALIVIVCLTAIQAIGTN
                     ANATFDKVATSLPAGSS"
     regulatory      complement(11773..11864)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(12209..12292)
                     /locus_tag="VFV87_13650"
     tRNA            complement(12209..12292)
                     /locus_tag="VFV87_13650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12256..12258),aa:Leu,seq:gag)
     gene            complement(12954..14474)
                     /locus_tag="VFV87_13655"
     CDS             complement(12954..14474)
                     /locus_tag="VFV87_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5104858.1"
                     /translation="MTDSASAGLRLFVEEPPEEPAALCLPQSAALFRAFEQATGWHVG
                     LEQAPPGPGEVWSTTIDTLAGQRPSRLVLRPAAHEEQNSGTASPPIDLHLARPLALAI
                     GGFLGEFNRVQRAVWQREAELAAGVPVAIRAGDEPHLAERLEGVIKSGAEAVGCQAAG
                     LYLLDEHTSHLKLRAAWNLPRERFLAEPLPLRGAIADLEALVGHAVVLEDTSVLPHWR
                     CPEDFPAAVCVPVSSSTTPLGTLWVFSGEKRDFSPEETNLVEIIAGRLASDLEREMLL
                     AAGLQLKTTDRQLDAAARWLSARLPSVMPLAEGYEVAGWTQQAAEIGGAFHDWSVLPD
                     GRLALAAGAAAGQGAEAALGAASLHATIKSHTGYRHTAAELLSRVNQTLYTSSPGDQR
                     ASLAYALIDPDRGAVDLALAGSARAHVIGKRNRPFGNAEIPQLGESLEAVFPAKRMLL
                     GPAETLLLVSGAQRVDDSVLPAAVSANQTVERLREQLANRTNDRAAVVLKRQYRGH"
     gene            complement(14668..>15087)
                     /locus_tag="VFV87_13660"
     CDS             complement(14668..>15087)
                     /locus_tag="VFV87_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5104859.1"
                     /translation="VRELRNAIEHALILAKGGVMLPEHLPPPMPPLVQAELAASSAGA
                     IALPASDTGASRSEADQLRTLIEAWARERLRAGAQPTGTQPEDLYDQFLALVEPPFLE
                     AALRKHHNQCAAAARALGIHRTTLKKKLDQYGIAGEE"
BASE COUNT         2872 a         4719 c         4806 g         2690 t
ORIGIN      
        1 ggcgatcagg gcgcggtcga aactgcggct gccctccatc agctcacgca gatgggcaca
       61 atgattggga cgcccgacta catcagcccc gaacaggccg gcgatgcccg caaagctgac
      121 atccgggccg acatctacag cctgggctgc acgctctata cgctgctagc cggccgcgcc
      181 cccttcggtg aaggatcggt cctcgagaag atcaaggccc acagcgagca ggaagcccgg
      241 ccgctggccg acttccgcaa cgacgtgccg gctgaagtcg aggccatcgt cagaaagatg
      301 ctggccaaag acccggccga ccgctttcag actccgtcgg aagttgccgc ggccctggaa
      361 aagtgggcga caaaccagcc gctttccgcc actgccaatc aggcccgagc ccgccgccgg
      421 gcgctcgcgc tgacggcgct cgcaacgatc atttcggcaa ttgccgctgg cgtgctcatc
      481 tatctcgcca ccgacaccgg ccgactggag atcaacagcg acgtggagat tcccggagcg
      541 aagatcgttc tgaccaagaa tgggcaagag tatgcctcgt tcgacgtcga gcccggcgcc
      601 aagcagcaga caatccgcgc cggtgattac gaggtgtcac tcagcggcgc tcccccaggc
      661 gtcgatctgg atgtcagatc cacgaagagg ggcaagccac gcgactatgg cgcgccctac
      721 agccccaaag acaagatcat cgccgtttat cgcggcggcg gaatgagtat tgaaatcgcc
      781 aagcaactca caccggcgac agcggttccc atccaggatc cgaacgcgcc aagcgagttc
      841 ttccaagtgc ttggcgaact ccaagccatc gtccgcaaag ccctcatcga agaaggcctc
      901 acggtggggc aattcaatcc tggcacgcag gtcgcgggtc cgcgccggtt gattgttctt
      961 caggccgaag cgaccggcga gcgctacgac caaaacaagg tcctcacagc actccgcaac
     1021 gagttcaagg cgctcgcaac ccaaaacgat gccttcgtct ccagcctcga ccaccagatg
     1081 cacggaggcg tcgcgttcac caatctgccc tataccacgc cggcgcgaga agggctgatc
     1141 cgcgtgacga tgcgcagcct cggcaccgac aaacgagaaa tcacaattgc caaatggagg
     1201 ctggtcatcc aggtcgagga atggccgctg acgaagggca acatcgccgc tccggatggc
     1261 caatcggata gtccgagcca gtattttgag cgtctggcgg gcgaaccgtt ggagtccgcg
     1321 gtgcgggagc gcgttggcgg cgctgtgggc agacgtatca cgagcgagcg aattccgcag
     1381 ccgccgggaa agcttcgcta tgaggcgcag atcgatggcg tgggcagcaa gatcatcgac
     1441 caggtgtttg tcgatctccg cgatgtgctg atgcggggga cgggcgcccg gattgttcac
     1501 gaaggcgaga gcggcatcga tctgcggctg ctgcggtaca cgacaccgac gcacaacgga
     1561 gaggttcgcg tcaccctgat cagccgcgaa ctgggcggcc agccggccga cgagagcacg
     1621 aagtggctgc tcaagattga cgcttccgag tgggccaagg gcaccagcca ggcgtcgccc
     1681 tctgtcgtgc gcgtggaggg cagcggcgag cagatgatac tctcgctcga cggtgcgccg
     1741 ctgacgcgcg cggaattgct cttgcggctc gacgctttgg ccaccggcga tccgggcttt
     1801 ctggtctacg tcgaaggaga tgtggttcca gtgacgaagg acgtaacgga cttgctgaag
     1861 gacatcggcg ctacggccgt tggcgaaaag aatgtgagat ttccctcgga agtcaccggt
     1921 caaatcatgg acgacgccgt gcggcgcggg aaagccgagg cggctgaacg caaccaaccg
     1981 cccacggcgt cgagtgcgtc gctcacggaa ggacagatca aggagcttgt ttccgaagca
     2041 gccggcattc cgaatgacga ttggacgaag ctgacgagcg gtatggaggt gccatcgccg
     2101 aatacgctcc agagccagac attgtcactg atgctgttca gcctgcgggc gctgccgccg
     2161 gcggggattg agagcgagtt gccggcgatt gcgaacctga agaaagactt tcagtatctg
     2221 cctgagcaga cgccgaagcc ggcggagatc gcccgggcgc tgtggatcag caagtcgaag
     2281 ggttatgctt cgtatattca gcccgagtat gtgactgaag tgacggtgca ggttgccggc
     2341 gacacggcca gcggcgaggt cgcattcgag aaggaggggc tgtaccgcgg ccgagtccgc
     2401 tacgtggccc gacgagccga cggagattgg cggatcgagg agttttatct gccgaacgtc
     2461 gggctgtcgc tagtgcggaa cgagcaaggc gtgtgggagc ggatgattca ggtccaggaa
     2521 agtccgaatt gacggccatg agcgaatcgc gttgccggcg gacgatctac gtgtcggtgc
     2581 tagccctcag cgccggtgcg gcattgacgg gcgaaacggt atctgccgcc cggctgctga
     2641 aggccgagat cgaggtggat ggaaagctcg tgctccagac agcctattcc gacgatggca
     2701 ctgagcaagt ggcgacagtg tggcgctatc tggcgcgcca cctcgtggcg gcgcattcaa
     2761 tcaggggatg ctgatcgcgc tgctcgccgg ccttgcgata attgcgacgc tcgctggagt
     2821 gctggtctgg attgcctggc cgcgacgtcg catcgccgaa cggtcctaga actgtagcgt
     2881 cgggacgaaa cagaagatgg ggatcagcag cgaggcgtag ccgagtgtgg tcctcagcca
     2941 gccgagtcgg acgctgtcgt cgcgcgtcgg cggatgatcg gtgccgatga acagtacgag
     3001 ggcaatcatc agtacccatg ccggcggcac gccgaagccg aagaacatca ccatgacgta
     3061 aagacaggct acggccataa aaccccgagc tagccaatga gccttccgac cgagcagggc
     3121 ataggccacg tggccgccgt cgagctggct caccggaagc atattaagcc cggtgaccaa
     3181 gagccccacc cagccggcca tgaaccaggc gttggcacgc gtaagggcaa agccggtgcc
     3241 cggatcgtac ttgcccggat ggagatattc gatcgccagg cgcatcgcca gcggcacctg
     3301 caggttgtcg ccgcttggcg ggaccggtgt caggtccatg tgctgcacgc cgatccacat
     3361 gatcggcagg gcgacgacca gtccggcgag cggtcccgcc aggccgatat caaagatttg
     3421 cctgcggttg gcaatacgac tgtccatggc gatgaccgca cccatcgtgc cgatcggtga
     3481 aaccggcagc gggacgaaga atggcaggct ggcccgcacg cgatagacca gcgtcatgac
     3541 gaagtggccc atctcgtgcg tcagcaggat tgccagcacg caccccatgt agagcaagcc
     3601 ctcccgccag ttggccagaa tcgctcgacg cagcggcatc aagctgggct cggtagggac
     3661 gccaccggta ttcgtgatgc cgaagtccag tgcccggaac atatactcat gcggcacata
     3721 cgacgttgcc cccgccagaa acgtcgagcc gcaagtcagc acgaagagcg caattggcag
     3781 tccaacgcgg cggcggtaat accgggccgg cggctgctca tcgacttctg gcacatcggc
     3841 tgtttcagcc ggattcgccg aggcagcctc cggcggcgtc ggctccgacg ccacatccac
     3901 gctggcgccc tgccccgtcg cttgagattc caggccggct actggttggt tcgggacggg
     3961 gctggtcgaa gacatcgaat ccttgtggtt tgcggagcgt ccacccgatt cattctacgc
     4021 catcgtccgc cgagcgcaag cgggcccgaa tcgccttcag ccgctcggcc agctccacct
     4081 caaagccgcg actcaccggg tgatagtatt cgcgctggac acctagatag tcctgggcgg
     4141 caacgccccc gtcggcttcg tgggcatact cgtagccttc gccgtggccg aggcgcttcg
     4201 ccccggcgta gtgcgaatcg cggaggtgga ccggcaccgg cagaatgcgg ccgtcgcgca
     4261 cgtcgcgacg ggcttcgccg atggccagcg tcgacgcatt cgatttggga gcacacgcca
     4321 ggtacgtcac ggcttgcgcc agtggaagct ggcattccgg cagcccgacg aactcacacg
     4381 cctgcatcgc cgccacggct aagggtaagg cctgcggatc ggcgttgcct acgtcttcgc
     4441 tggccagaat aatcagccga cggcagagga accgcacatc ttcacccgct tcgagcatcc
     4501 gagccagcca gtacagcccc gcatcgggat cgctgccgcg gatgctcttg atcaaggcgc
     4561 tggcatggtc gtagtgctcg tcgccggtcg cgtcgtaggc gccggctttg cgttggaccg
     4621 actcggcagc cagttcgcgg ttgaagtcga taggcgccga cgttccttcg ctcggctgag
     4681 aatgtcgtcc ctgacgttcc gggacgccag cgacggcatt ccccacagag agactcgaga
     4741 gcactccgac ctccagtgca ctcaaggcac gccgggcgtc gccgtcggat acttgggcga
     4801 ggaattctag cgcatcgtcg tgcaggtgaa cgtcatagat gcccagcccg cgcgttttat
     4861 ccgcgagtgc tcgccgtata atggtcttga tctcctcggg cgaaagtggt tggaactcga
     4921 aaatctggct gcggctgacc agggcgctat tcacagcgaa gaacgggttg ctggtcgtcg
     4981 cgccgacgag cgtgacgatt ccctcttcga cgtcaggcag cagggcatct tgctgggctt
     5041 tgttgaagcg atgcatttcg tcgatgaaca ggagcgttcg ccggccactg ccggccaggt
     5101 catcgcgagc cgcctccaac acctcgcgta attccttcac cccgctggtg actgccgaga
     5161 gctgcttgaa tctcttgcgg ctttcggtcg ccagcagacg ggcgagagtc gttttgcccg
     5221 ttccgggggg gccatagaaa atcaccgacg ataatcgatc cgcagccagc aaccgctgga
     5281 gcagcttgcc tgtccccaga aagcgctgtt ggccgacgaa atcacccagc gactgggggc
     5341 gcattcgcgc cgccaacggc tgggcctggc ggaggttgtc cgcttcgctt gtctcaaaga
     5401 gtgaaggcat caagccattg taggcactcc gctcgtttag cggcgagcca gcggccatgc
     5461 ccccgttttg acacgcctga tcggcgtcgc atcgggtccg cgttacacga cgcctggcgg
     5521 attagagcgt cttccccacg gcctacttct tccccttgcc gcccaggaag gcgagccagc
     5581 taccggtcgc cgccttcgcg gcagcctgat cggcgggggt atcgctcttg cgattgtcgc
     5641 tggtcaggtt ctccgcgagg gcggagatcg cctgcgtgat actcgcccgc ggagcctgct
     5701 cgatgagcgg cacgccgtta ttgcggacct cgaccatcgt gcgatagtcg ttgggaagct
     5761 gccagaagat ctcgcggccc attgtttcct gggctttctt caagctgatg acgcccgcgt
     5821 ccaggcccac acggttgacg acgatcttga ccttatcctt gagcccttcc acctcgttaa
     5881 acgacatcat cagccgcaca acgttccgca ggcaaggcag atcgagctgc gtcaccaaca
     5941 ggatgtcttt ggcagacttc agcgccacga cgtcgatggg cgagaagctc ttggagacgt
     6001 cgagaatcat gtgggtgaac gacgccttga gcagaccgag gacgcgggtc aggtcttcag
     6061 ccgtgatcag cgatccatcc tggagctgca cggggcgggg caagaggtac aggcccgagg
     6121 cgtgcttggt cagcgatcgc ttgagcagcg tgaagtcgag ccgcgagatg ttctgagcga
     6181 catcgacgag tgtgtagtcg ggaatggcgt cgaggaacac gtcggcatca cccagagcga
     6241 ggtccagatc gacgagcacc gcgctattgc gctgctcgct ggcgaggagg cagcccaggt
     6301 tgaccgccag gctggtcgaa cccacaccgc ccgtcgcccc gcagacggcg atcacgtggc
     6361 acgtgcggct gcggccgtcg ccggcgccga acttcgccga gctgatgcgg tcgagagcgg
     6421 aaatcagatc ctcgaccttg acgggcaggg tcaggaactc cttggccccg gcccgcatcg
     6481 ccttgagaat cacctgcccg tcggtcgagc tgctgacgac caggaccgag cattccgggg
     6541 cgatgtcccg gaccgattcg atcagtttga gccccttctc ggggttggcg tcgattgtaa
     6601 tgatgccgat gtcgggcttc gtttgcccga ccacgtcggc aaagaattca tagcgggaac
     6661 attcggcctc cagccagacg atgtccattc ccagcagcat gtttttgagc tgcgtgcgcg
     6721 aattatcgct gggatcgacg atcgcaagtc ggagaacgtt gctcatcgcg gaggggcagt
     6781 ctcggggcgc gcggcggcag gaaacctgct gccgaatcaa cagcgctggc gctacatata
     6841 tcctcttacc ttaaatcgtc gtacccgagc ggcccaatca aggttggctg tgcggcagtc
     6901 gagggtttct gcgactggcc gctcttctgg taagagctgt aaccgttttg cggcgaagca
     6961 gttgcgctgc ccgtgccgac cgtgcgggtt gcagcggtcg gacgccgcga attggccgcg
     7021 gtgctcttga tcaccggcgc cggcttgcgc tgggcgctgg agggggcggg cacaggcggc
     7081 ggtggcgcct gcgggatcgc ctgtccaggc gggagctctt catacatggg gccgtcgtag
     7141 ccgccatgcc cgggaccgcc acctttggga acctccatgt agccacggaa gtacagctca
     7201 caatcggtcg ggctggtggt caactcgccg gggccacatg gcggcacttc tcccggatcc
     7261 atcgcatcgc agaattccgg tgtgacgaag atcagcaact cgacttcatt cttccgctcc
     7321 gaaacgcggc ggaaggcagc cccggcccac ggcatgtcgg ccagccaggg caggccgcga
     7381 ttggtcgcct caatccggtc gtacacgaga ccggcgatgg ccagcgtctg tccggctttc
     7441 atttcgaccg cggtatcggc agttcgagtc cggaagccag ggatgccggt gacctggtcg
     7501 cgaaggctgg gatcgacctc cgtaaccgaa ggccgaactt ccaggcggac caggccgttg
     7561 ccgaggacga cgggtacaaa gtcgatccgg gtgccgaact cacggtattg aacggtcgta
     7621 acacccagcg cctgctggat cggaattggc acttcaccac cgacgttgaa gctcgccggc
     7681 cggccggaga gtgtagtcaa cgtgggctct gccatcagct tcgccagatc gtattgacgg
     7741 agcgcttcca ggaagccgta gaactgtccg ccgtctccga tgatgccgaa gcgaaccgtg
     7801 gcgccgggac cgagcagcgt cccaggagac tgcagcaggc ccgatacgtt ctgcgtaata
     7861 aaaccacccg atgagatatg ggcccagtcg aagcccaggc tccgcagctt ggtgcgggag
     7921 acttccatca ctttgacgtg gagcagcacc ttatggacgc cgccgacggt catgtcatta
     7981 atgacctggg ggaagtaatc ctgtgccacg cggacgatgc tgttgaccat ttcggccttg
     8041 ggcacaaagc ccgagatata taggctgctg ttgagctggc ggaggcggag cgaggcctcg
     8101 ggaaagagcg ttttgagagt cccgtcgagc tcctgcacgt cgcccaggat ctgcaggtcg
     8161 atcgacgtga cgtttccctc cacgtcccag acgttgagct gagtcacgcc agcccggacc
     8221 gcggacagct ggatcgaccg gggcgagatt ggaatcactc ggaccaggtc gggattattg
     8281 accagcatcc gcggcacgtc gaacgggaat tcgacgaccc gactagagtt gacggtcatt
     8341 tccagacgct cggcagcacc gcgcacctga aaggtaacgc ccgggcctcc tggctgggcc
     8401 tgcgcggaaa gccgccccgc ggttgcgagg atgatgccaa aggcgataag cagggccaac
     8461 gaggccttgt aggagacgat agacatccgt gtgcttcctt gcgtcctggg taaacgcgag
     8521 taatcggtcc gtgaatatcg atccgtcgag gtgactaggg gttactccag cgacagcgca
     8581 cttccgttgt cgctgggacg ttcggtcgtc gattcagcgc tgccatcggc tggggtgttg
     8641 gactgggagg gcgcggactg agccggcgcc gatgtcggcg tgggtacgag cgtgctggtg
     8701 tcgccggcaa aggcccccga cttttgtggg agtccatcgc gcttcgccca gttgtactgc
     8761 tcgatgccgc cgggggtcat gatgtccagc ttccattcgc tgccgctccc ggaggaggag
     8821 ccctgggcgc cggagctgag gaactgagaa aggcctgcac tctgctgggg ggctggcgcg
     8881 ggcgtcgggt cgccgccaag ctgcgacctg ccggccatca actcggacat cttcaggacc
     8941 tcttcgccta agccgtcatc gggctcgttg ggccgacgga ggctgagctg aatgcgaccc
     9001 atctgcttgg cgagttggag ttctgcaatt tgcttttcct tcaccaggag ggaaacggtg
     9061 cggaaggcgg cctcaccgcc cttggcatcg accgtccgtt cggtgttcga gttgacggcg
     9121 aagatctgca cgttccgcaa aatcgggaaa acgccggtaa ccggaatttc gcggccgtcc
     9181 ttcttcagga agacgctgac atcgactcga tcgccgggcg agattgcctt catgaccgta
     9241 tcttcttcca ccttcaccgg ctctgcgcga taaccctcgg gaatgtattg ggcaacgcca
     9301 ctggcctggt cggccagctt ggagatcaga atgggctcgc ccttgtagaa ccgcgtggcg
     9361 gtgaatttgt ccttggcgtc gtcgagccgg cgaatcgccc cctcgggaac cttggcctta
     9421 ggccaatcct cgagcctgac gttggtggca tcgagctttt tgttattgtc gatgtcggcc
     9481 agggccacga gaatttgctc catctccatg ccgggaccgg acgagccgcc gcgctccatc
     9541 acctggctaa ttccgatcga cgccaccagg ccgcagccta gggcgatgaa aatcaggatc
     9601 agcgacttca ttcgcatcgg accatacctc gaccgacgag ttcagggcgc gggtgagtac
     9661 gttttggcgg tgccgagcat tccaaggtat gaatgctccg gcccgagcta ctggactgtc
     9721 tcgcggcgca tcaccgtgct ggcagtcagc gtttttcccc caaggtacat tggcgatggc
     9781 agccaactca cttgatcgaa tccaatggag accgtaaccg tcacagggtc gccaaattcc
     9841 gcatcactaa gcggatctgg cgcgaccgtt accgtcgcac cggaaaccga gccagttgcc
     9901 aggtagccgt tgaccgtgtt ctgcacctcc gccacgtcgg ttccgtctaa aacggccgcc
     9961 cgcgctccct cgcgcgacgc gttggtgatc acctgctgaa tcatgaccat gcggccatat
    10021 tcgatcatgc cgaatatcag caggaaaaac accggcgcga cgatggcaaa ctctaccgcc
    10081 gccgcggccc gtcgttttct tcggtacgac cggcaaggtt tttccaactt atgagaggcg
    10141 gccgctaaaa tcccgccaac cggcccagat tgccgcccga ttgatagcaa attcgcgata
    10201 gcagtttggt aaaggcggtg gatcatacga gcatccccat ccacaggaag taaccgattg
    10261 ttccgatcgc aatcggaatg ccgtagggca gtagcatcat tgagggcttg cgcctggcag
    10321 cgatcgccga gagctgttcc ggattgcgga tcaccattat ttcgttgagg atcaccatga
    10381 actgggcgga gtgcttcttc caaccccgct ggatcaacac catcaccacg gccagcagtg
    10441 cgcccgcgac ggcagtcgca gcaaaagcat aggcagtgtg gatcccatag acccaggccc
    10501 ccacgccagc cagcaacttc acgtcgccgg cgcccatgcc gccgatggcg tacgccggca
    10561 atagcagggc caggcccaca accgttccca gcaggctcca gcccagccct tcccatccga
    10621 aggccacggt gctgtacacc cagccgctga ggatgaaggg gaaggtgacc caattgggca
    10681 ctttgagctc gaagccgtcg atcacggccg ccacaatcag cacaatggtg acgagccaca
    10741 cggtccagtg ctcggcaatc gccgaggcat aagcggacat cagttccatg gcagtagctt
    10801 tcttccccgt gacaactagc ggatcatgaa cagatagcta aaaaacgccg ccgcgagcgt
    10861 gcaaccgcag caaatcaatt cccacccgcc gatcagcagg tcgtggggca tgaatggcaa
    10921 catggcggca cctcgctcag cggaactcta gcttgctgtg gcgctgatgg gcggctgggt
    10981 ggattcaagt ccaaacccag ccacccatag caaacggtcc actacgagct gccggccggc
    11041 aggctcgtgg cgattttgtc gaacgtggcg ttcgcgttgg tgccgatggc ctgaatggaa
    11101 accaggcaga ccacgacgat caaagcgagc atcacggcgt actcgacggc ggtcgggccg
    11161 tcttcattga ccaggaaact acgaacgttg caaagcaact tgttcatgac gaaacccttg
    11221 cgactagcgt gacgagcgaa cggaggccca cgcgggcccc tctaaggctt tctcaccctg
    11281 tccgatgtgt attgcatcga aggcgcacag ccctgggcgt tgcggatgcg ccgcctgggg
    11341 atggtgagca tgcaccgtgg ggacgattgc gggtcatacg cactcgctac gaggatcctg
    11401 cgggcaggct cgtagccacc ttgtcgaacg tggcgttggc gttggtgccg atcgcctgga
    11461 tggcggtcaa gcacacaatc acgatcagcg ccagcatgac ggcgtattcc accgcggtcg
    11521 ggccatcctc cgaggccagg aaacgttgca ccctcaaagt cagtttcttc attgcttcct
    11581 cccgtgctcg agccgcggca tccgtcgccg cagccttcac cgaatgaatc ccgcccgccg
    11641 ccgcgactgt tgccagtcgc cggcctgccc cgcgccgggt cgagattcaa actaaaagtc
    11701 gcctcgtcat cgtcgctcgt gaaaacaggc aatcacgccc cgcgggtcgc accgcgaaac
    11761 gcaatccacc ctttcggcag ccgggcgacc gccccgcggg aaacccgcca ggcggccctc
    11821 tggctttgcg ccccggcatt gctgccggtt tgcctttgtc gaggatgtga ggcggcaggc
    11881 ctgtaaacag gcaagcccaa cgcagatcga attgcggacg cgaatgcgcc ccttcatcga
    11941 aagacgagca cttgccggtt ggaaactgcg ctcggccttg cagccgaatg ccgcttccga
    12001 atgaaaccta ggtcaggcgg cgaagctgtc aaatttgcag ctttaggaaa tctggcaaga
    12061 atcctcagat ttttccgagg gcctgtcatt agggacgggc tgaccccggt tggagcgcaa
    12121 ccacgcattc tgcaacgcct gcggttcgcc gttaaacgag aaaaccccga cgattgatca
    12181 tcggggttgc aggtgcgcca attgaaagtg cggtcgagag ggcttgaacc tccacgccct
    12241 tgcgggcgcc agcccctcaa gctggtgcgt ctgccaattc cgccacgacc gcgccaaaag
    12301 gcaactttag tctaggtgat ccgcacccgc ggtcaaggga cagcgcttgc aggctcacgg
    12361 ttcgctcgcc aagtttgcgg cccgtctacg cccagcgggc atctgcagtc aagcgtcgtc
    12421 agctcaatct ttagttcgcc gagtgattgc cgccacaacc agtcgaagtc gtaatacgcg
    12481 ggaggcagtt gtcgaacaga atgatgcggt gactcgctcg ccgacacgag aatctcccca
    12541 ggtggctctg accaaccgga ggcaatgatc tcagcgggtc gatttggcgt tggaaccggc
    12601 aaagtgcccg cgacgagcaa ggtgacaatc acaccggcaa cggctgtcga cagaacgaac
    12661 gtccggaggt acggcaacac gattcgttga ggcgtggatt gcgtcgcgtg actcgaacgc
    12721 tacgggttcc tcttctctgg tcgttcagta cgatgctgcg cgcggcggac cgagctacgg
    12781 cgcagtgaag aggaacccgt agttgcctgg atagccattc gctctacaca tcaaggagta
    12841 ttccagaggg ctgcctatgc cgcttcattg tcggcgactt gatggtcgat ctcggctgga
    12901 tggactcggg cgtagatcca ggcagcggcg aggtctttgg tacgcagaag tggctagtgg
    12961 ccgcgatact ggcgcttcag cacgactgct gcccggtcgt tggttcggtt ggcaagttgc
    13021 tctcgcagac gttcaaccgt ctggttggca gagacagccg cgggtaggac gctatcatcc
    13081 acacgctgcg cgccgctaac aagcagcagc gtttctgctg gaccgaggag cattcgcttc
    13141 gctggaaaga ctgcttcgag cgattcgcca agctgcggga tttccgcgtt tccgaacggt
    13201 ctgttgcgct tgccgatgac gtgcgctcga gcgctgccgg ctagcgcgag atcgaccgcg
    13261 ccgcggtcgg gatcgatgag ggcataggcc aggcttgctc gctggtcacc gggcgaggaa
    13321 gtgtagagcg tttgattcac gcggctgagt agctcggcgg cagtatggcg atagccagtg
    13381 tgggatttga tggtcgcgtg cagcgaggcg gcgccgagcg cggcttcggc gccctggccc
    13441 gctgcggctc cggcggccag cgccaggcgg ccgtcgggaa gcaccgacca atcatggaac
    13501 gccccgccaa tttccgctgc ctgctgcgtc cagccggcca cttcataacc ctcggccagc
    13561 ggcataacgc tcggcagccg agcggaaagc cagcgagcgg cggcatcgag ctggcggtcc
    13621 gtcgtcttga gctgcagccc ggccgccagc agcatttcgc gctccaggtc cgacgccagt
    13681 cggccggcga tgatctcgac caggttcgtc tcctccggcg aaaagtcccg cttttcgccg
    13741 ctaaagaccc acagcgtgcc gagtggcgtc gtgctgctgc tgaccggcac gcacaccgcg
    13801 gcgggaaaat cttccgggca gcgccagtgc ggcaggaccg acgtatcctc cagcacgacc
    13861 gcgtggccta ccagtgcctc cagatcggcg atcgctcccc tcagcggcag cggctcggcc
    13921 aggaaccgct cgcggggcag gttccacgcg gctcgcagct tcaggtggct cgtgtgctcg
    13981 tcgagcagat acagccccgc cgcctggcac cccaccgctt cggcaccgct tttgatcacc
    14041 ccttcaagcc gttcggcgag gtgtggctcg tcgccggcgc ggatcgcgac cggaacgccc
    14101 gcggccagct ccgcctcgcg ctgccacaca gcgcgctgta cccggttgaa ttcgcccaga
    14161 aagccgccga tcgccagcgc cagaggccgg gccaggtgca agtcgatcgg tggggatgcg
    14221 gtgccgctgt tttgctcctc gtgtgccgcc ggtcgcagca ctagtcggct gggccgctgg
    14281 ccggccagcg tgtcgatcgt cgtggaccag acctcgccgg ggcccggtgg ggcttgctcc
    14341 aatccaacat gccagccggt cgcttgctcg aaggcccgaa acagagcggc agactgggga
    14401 aggcacagcg cagcaggttc ctcgggaggc tcttccacga agagccgcag tccagcggag
    14461 gcagaatcgg tcacaggcga ggctcggcgg cgtggaggag tcgttgagcc gtccgtgcac
    14521 tggggcaggg ctgcgcgctc gtcgccccgc acggcctcca tgaccgttac aacttcccat
    14581 cgtcggcaag tgctgtgcat gtcgagtcaa accgccaagc gccgtagagc cggaaccgtc
    14641 gaaacaaccc gatccgttcg cccgcagtca ctcttccccc gcgatgccgt attgatcgag
    14701 cttcttcttg agcgtcgtgc ggtgaatgcc cagggcccgg gccgcggcgg cgcactggtt
    14761 gtggtgcttg cggagcgccg cttccaggaa ggggggctcg accagcgcca ggaactggtc
    14821 gtacaagtct tccggctggg tgccagtggg ctgggcgccg gctcgcaggc gctcgcgggc
    14881 ccaggcttcg atcaacgtgc gaagctgatc ggcttcggag cgcgacgcgc ccgtgtcgct
    14941 ggccgggagg gcaattgcgc ccgcggatga cgcggccaat tccgcctgca ccagcggcgg
    15001 catcggtggc ggcagatgct cgggcaacat cacgccccct ttggccagaa tcagcgcgtg
    15061 ctcgatcgcg ttccgcaatt cccgaac
//
LOCUS       DASUGB010000712         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1746810, whole genome shotgun sequence.
ACCESSION   DASUGB010000712 DASUGB010000000
VERSION     DASUGB010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746810"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFV87_13665"
     CDS             complement(<1..283)
                     /locus_tag="VFV87_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104860.1"
                     /translation="MLVGPIFTRELVTAPRRPQHFVYRTVYGIAVFGLMCTAWLVLVQ
                     TQIIRTVGDMARFGSNLFLILAALQLALMTFLAALRSASAVSQEKDKKTI"
     gene            665..1219
                     /locus_tag="VFV87_13670"
     CDS             665..1219
                     /locus_tag="VFV87_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5104861.1"
                     /translation="MSELDDKVDPAKVDRGKLDPSIVAALYVQHAEELRRFLLGILRD
                     AQLASDVLQATFVKLAERGQETREASRKAWLFRVAYHEAMAIRRREGVGDKVLRRVAW
                     HTNGAAGPAEEPLLRLESVELVREALDELPPEQRKIVRMRIYEEKTFATIAKQLKIPL
                     GTALARMRSAVMKLRAKLDQRSQE"
     gene            1249..1854
                     /locus_tag="VFV87_13675"
     CDS             1249..1854
                     /locus_tag="VFV87_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104862.1"
                     /translation="MRNEHKHEHDCQNQLDWSAFCYLSGEMSLTDTEQFEARLADDQA
                     AREALARMVELTQVVAAAETHAADLRPAGEKRTSWTARISWMAIGGLAAAVLAMLWSG
                     AGFWNEPSGENTGISRATNALASAWFETRRELRDADDIGPLHPIPLALSETDDESAAA
                     TGNTADDLLVADAPSWMTAALQGLAGEMDQDETGGDEAVVN"
     gene            1956..2642
                     /locus_tag="VFV87_13680"
     CDS             1956..2642
                     /locus_tag="VFV87_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104863.1"
                     /translation="MFRPAKVVALALTLALVGLLLGNATGQQGGKSETNKRETGKNKP
                     QAIAVTPEREAAVTTFVKRNHAELADLLAHLKANQPQEYERAVRDLYRITERLASIQE
                     RDPTQYDLELTLWTAQSRVQLFTAKLKMGTTDELKSELRQALDAQAEARVALLKHERT
                     KVVDRLSRIDGDIAQLEGEREKLIDKQLELLVRTARQGKEVKLGPKGAVSKPVNKPSA
                     KRAGSKTSAD"
     gene            2734..>3121
                     /locus_tag="VFV87_13685"
     CDS             2734..>3121
                     /locus_tag="VFV87_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104864.1"
                     /translation="MPVDLRKIVSLAVLALVALASRAALAEGEALPPVNERYAKDDVS
                     EEPDFQRHVSPLFGRLGCNGRSCHGSFQGRGGFRLSLFGYDFKADHDELLKGEPLRAN
                     KDKPLESLILVKPTDADMHEGGLRYKQ"
BASE COUNT          652 a          948 c         1032 g          489 t
ORIGIN      
        1 ggatcgtctt cttgtccttc tcttgcgaaa cggcgctggc cgagcgaagc gcggcgagga
       61 aggtcatcag cgcgagctgc agtgcggcca ggatcaggaa caaattcgag ccgaagcggg
      121 ccatgtcgcc gacggtgcgg atgatctgcg tctggaccag cacgagccaa gccgtacaca
      181 tgagtccaaa gaccgcaatc ccatagacgg tccgatagac gaagtgctgc ggccggcgcg
      241 gagccgtcac aagttcgcgg gtgaagatcg ggccgacgag cacgggggag gcagcggggt
      301 tcggggctcg gggttgggga ccaaagagga caggagagcg gggacagact acactctgga
      361 tttgccgcgg attgtagcag tggtggtacg ggccaggcag gacgggcgtt cgggcgcggg
      421 cgagacgcag gccgccgtct atagtttaac ccgtagccag ttcgtttaac cggttagccg
      481 aaggcacccc gctggcccgt acgctgggca acaacggcat cgcctgcggc tcgccgtcaa
      541 acgaatctac gcgacgcaag gcattggcac agcataactt acaaaaatcc agaaaataat
      601 tccccgccac agcgcatcag agtctgtgag cgtccagtgc cgagcggtcg gcaggagcgc
      661 ttgagtgtca gagttggacg acaaggttga cccagcgaag gtagatcgag gcaagctcga
      721 cccgtcgatc gtggcggccc tgtacgtcca gcatgcggag gagttgcgtc ggtttctgct
      781 cggcatcctg cgggatgcgc agttggcttc cgatgtgctc caggccacgt tcgtcaagtt
      841 ggcggagcgg ggccaggaaa cgcgggaggc gtcgcgcaag gcgtggctgt ttcgcgtggc
      901 ctaccacgag gcgatggcca tccgccggcg cgagggcgtc ggagataagg tccttcgccg
      961 cgtcgcctgg cacaccaacg gagccgccgg accggcggag gagccgctcc tgcggctgga
     1021 atcggtggag ctggttcggg aagccttgga cgagcttccg ccggagcagc gaaagatcgt
     1081 tcggatgcga atttacgagg aaaagacctt tgcgacgatc gccaaacagt tgaagattcc
     1141 gctcggcacg gctctggccc gcatgcggtc agccgtgatg aagttgcggg cgaagctcga
     1201 ccagcgatcg caagaatagg cgatcgaaag aaaaataaga gtgacgaagt gaggaacgaa
     1261 cacaaacacg aacacgattg ccagaaccag ctcgactggt cggccttttg ctacctgtcg
     1321 ggtgaaatga gcttgactga cactgagcag ttcgaggccc gcctggccga cgatcaagcg
     1381 gcccgcgagg cgctggcccg aatggtcgag ctcacgcagg tcgtcgccgc ggctgaaacg
     1441 catgctgctg acctgaggcc cgccggcgaa aaacgcacaa gctggacggc tcgtatctcc
     1501 tggatggcga tcggcggcct cgccgccgca gtgctggcga tgctctggtc gggcgccggt
     1561 ttttggaatg agccgagcgg cgaaaacacc gggatttcgc gggcaaccaa tgctttggcc
     1621 tccgcctggt tcgagacccg cagggaattg agggatgccg acgacatcgg cccgctgcac
     1681 ccgattccgc tcgccttgtc cgagacggac gacgaatcgg ctgctgccac gggtaacacc
     1741 gccgacgacc tgctcgttgc cgacgctccc tcttggatga cggctgccct ccagggtctg
     1801 gccggcgaaa tggaccagga cgaaaccggt ggcgacgaag ccgttgtgaa ctaaccccgt
     1861 agccgcgttc gccagaacgc gggaaaccaa ttacctcgat cgtgaccggc ccgcactctg
     1921 gcgcgtgcgg ctaccagcga gaaagcgcgt ttgcaatgtt ccgccccgcg aaagttgtcg
     1981 ccctggcact gacgctggcc cttgtgggcc tgctcctggg caatgccacg ggccagcagg
     2041 gcggcaagag tgaaacgaac aagcgcgaaa caggcaagaa taagccgcag gccatcgcgg
     2101 tcactcccga gcgcgaggca gcagtgacga cgttcgtcaa gcgcaaccat gccgagctgg
     2161 ccgacctgct cgcccacctg aaggcgaatc agccgcagga atacgagcgg gccgttcgcg
     2221 acctgtaccg catcacggag cggctggcga gcattcaaga gcgcgacccg actcagtacg
     2281 acctggagtt gaccctgtgg acggcccaat cgcgcgtgca gcttttcacg gccaagctca
     2341 agatgggaac gaccgacgag ctcaagagcg agctccgcca ggcgctcgac gcccaggccg
     2401 aggcgcgagt cgccctgctc aagcacgaac gcaccaaggt cgtcgatcgg ctgagccgca
     2461 tcgacggcga tatcgcccag ctcgagggcg agcgggaaaa actgatcgac aagcagctcg
     2521 agttgctcgt ccgcaccgcc aggcagggaa aggaagtgaa gctcggcccg aaaggtgcag
     2581 tcagcaaacc ggtcaataaa ccttccgcga agcgcgccgg gagcaaaact tccgccgact
     2641 gaaccgctta aatgattggt tatttgggaa cccctaccaa tttcgttcca gccgctgccc
     2701 acctgcggcg ttcggacctt atggaggatc gatatgccag ttgatctaag gaaaattgta
     2761 tccctggccg tactggcgct ggtcgccctg gccagccggg ctgcgctggc cgagggcgag
     2821 gcgctgccgc ccgtcaacga gcggtacgcc aaggacgacg tgagcgagga acccgacttc
     2881 cagcggcacg tcagccccct gtttggccga ctgggctgca atggccgctc gtgccacggc
     2941 tcgttccagg gccggggcgg ctttcgtctc tcgctgttcg gctacgactt taaggccgac
     3001 cacgacgagc tcctcaaggg cgaaccgctg cgggccaaca aggacaagcc gctcgaaagc
     3061 ctgatcctcg tcaagccgac cgatgccgac atgcacgaag gcggcctgcg gtataagcag
     3121 g
//
LOCUS       DASUGB010000713         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_913026, whole genome shotgun sequence.
ACCESSION   DASUGB010000713 DASUGB010000000
VERSION     DASUGB010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913026"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..>2408
                     /locus_tag="VFV87_13690"
     CDS             86..>2408
                     /locus_tag="VFV87_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104865.1"
                     /translation="MKRTILLAAVAALFASALAAQQPAAPPVAEAPPVDPIGQQAAAL
                     EVELGKFKDTSPEAAEAMVKLVDLYHADGRLFGLVRVGQQFVAAHPADSRHQAVMLKL
                     IDGQEALSRNKELSATIRQFLARYPQAAECAPLEVRLAEALLQQEDRLRAAEACQAVW
                     QRQGANEIGRRYGAAGVPVFSAIGSGESIALAAALGEDMLDKLPPGDFARQVGHQAFY
                     EYRRISQWAKSNGVGAKLFAKGLAGDAETQRQLHVWMAENQANQGQFANAAQSLRQAR
                     AIRDDQYVHYLLLNRLYNGGAKPPEIEPLANEYLSKYPTRPDRFHGISWLAISCVQNG
                     EKPRAANLFASLLAEDPVFNGNASYFVQNNGSEAANFADSEAKLLAAVAQNKPGVHYL
                     RYVLALEVYRDRLQQPAKAKQIARELISQSPTDDGHTSGPLEWLLNNPIDDNEFKSDL
                     ALILAARQKNPHLANFREMVKNWAGAARQNKDLVARANLALEALKTANADPVLTMFVD
                     QRNNQHAPGEAIRNKLLEPAIFNTLSDGAARYVLQTQAEWFRHYSPGAKRGEQVRVYQ
                     QFCQRFPQDTQAGWYWLETAVDYGKPEEMKAAAENYLKFPPEASVADAWRRLMIVAEK
                     NNNDQTIARNSWNWMQQAQQKFGPDPTYATNIGDQLLKFGLEAEAVQHWTTYMTANRN
                     HYESRDCASRLLARIMEPPQRIAFIQAMLAPDTDFYGRYSQWLADEHLKLGDVANFEA
                     VLKAGRAKQVERPMKYADWDLWQVASWFDAIRGN"
BASE COUNT          488 a          803 c          733 g          384 t
ORIGIN      
        1 ctgagaactg cgaattgacc atccaacgcg catcaaggac cgccacagag ggcggtccct
       61 acagcgccta acgatgccga cgaatatgaa gcgaacaatt ctcctggccg cggttgctgc
      121 ccttttcgca tcggcgctcg ccgcccagca acccgcagcg ccgcctgtcg cggaagcgcc
      181 gccggtcgat ccgatcggcc agcaggccgc ggccctcgaa gtcgagctcg gcaagttcaa
      241 ggacacttcg cccgaagcag cggaggcgat ggtcaagctg gtcgatctgt atcacgccga
      301 cggccggttg ttcgggctgg tgcgcgtggg gcagcagttc gtcgccgccc acccggctga
      361 ttctcggcat caggcagtga tgctcaagct gatcgacggc caggaagccc tctcgcgcaa
      421 caaggagctg tcggccacga tccgccagtt cctggcccgc tatccgcagg cagccgagtg
      481 cgcgccgctg gaagtgcggc tggccgaagc cctcctgcag caagaagacc gcctgcgtgc
      541 cgcggaggcc tgccaggccg tctggcagcg gcagggagcc aacgaaatcg gccggcgcta
      601 cggcgcagcc ggcgtgccgg tcttcagcgc cattggcagc ggcgagtcga tcgcgctggc
      661 cgccgcactc ggcgaagata tgctcgacaa gctccctccc ggcgattttg caaggcaggt
      721 cgggcaccag gcattttacg aataccgccg catcagccag tgggccaagt ccaacggcgt
      781 cggcgccaag ctcttcgcca agggcctggc cggcgatgcc gagacgcagc ggcagcttca
      841 cgtgtggatg gccgagaacc aggccaacca gggccagttt gccaatgccg cccagagcct
      901 ccgccaggcc cgcgcgattc gcgacgacca gtacgttcac tatctgctgc tcaatcgcct
      961 ctataacggc ggagccaagc cgccggaaat cgagccgctc gcgaatgagt atctctcgaa
     1021 atatcccact cgacccgatc ggttccacgg catcagttgg ctggcgattt cctgcgtgca
     1081 aaacggcgag aagccgcggg cggccaatct gttcgcctcg ctcctggccg aagacccggt
     1141 tttcaatggc aatgcgtcgt atttcgtgca gaacaacggc agcgaggccg ccaacttcgc
     1201 ggacagcgag gcgaagctgc tcgcggccgt cgcccagaac aagccgggcg tgcattactt
     1261 gcggtacgtg ctggcgctgg aagtttatcg cgatcgcctt cagcagccgg cgaaagccaa
     1321 gcagatcgcc cgcgagctga tttcgcagtc gcccaccgat gacggtcaca cctccggccc
     1381 gctggaatgg ctgctcaaca acccgatcga cgacaacgag ttcaaaagcg acctcgcgtt
     1441 gatcctcgcc gcccgccaga agaacccgca cctggcaaac ttccgcgaga tggtgaaaaa
     1501 ctgggctggc gccgcccgcc agaacaagga tctggtcgcc cgggccaacc tcgccctgga
     1561 ggcgctcaag acggccaatg ccgatcccgt gctgacgatg tttgtcgatc agcgtaacaa
     1621 ccagcatgca ccaggcgaag cgatccgcaa taagctcctg gagccggcga ttttcaatac
     1681 gctcagcgac ggcgcagctc gttacgtgct gcaaacgcaa gccgagtggt tccggcatta
     1741 ttcgcccggc gcgaagcgcg gcgagcaggt ccgcgtctat cagcagttct gccagcgctt
     1801 ccctcaggat acccaagccg gttggtactg gctcgaaacg gccgtcgatt acggcaagcc
     1861 ggaggaaatg aaggcggcgg cggagaacta cctcaagttt ccgccggaag cgtcggtcgc
     1921 cgatgcctgg cggaggctga tgatcgtcgc cgagaaaaac aataacgatc agacgatcgc
     1981 gcgcaactcc tggaactgga tgcagcaggc ccagcagaag ttcggccccg atccgacgta
     2041 tgccacgaac atcggcgacc agttgctcaa attcggcctg gaagccgagg ccgtccagca
     2101 ctggacgacc tacatgaccg caaatcgcaa tcactacgag tcgcgcgatt gcgcctctcg
     2161 tttgctcgcc cgcatcatgg agccgccgca gcggatcgcc ttcatccagg cgatgctcgc
     2221 ccccgacacc gatttctacg gccgctacag ccagtggctg gccgatgagc acctcaagct
     2281 cggcgacgtc gccaatttcg aagccgtctt gaaagccggc cgcgccaagc aagtcgagcg
     2341 gccgatgaaa tatgccgact gggacttatg gcaggtcgcg agctggtttg acgccatccg
     2401 cggcaata
//
LOCUS       DASUGB010000714         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_78414,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000714 DASUGB010000000
VERSION     DASUGB010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78414"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFV87_13695"
     CDS             <1..275
                     /locus_tag="VFV87_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104866.1"
                     /translation="RFEGGSLNERVNVDPKSVTAADFRLRKDSAGYRAGPDGQDLGAD
                     VDLVGPGEAYERWRKTPEYQEWLKETRQKKESGDSSQESANGQSPN"
     gene            433..1554
                     /locus_tag="VFV87_13700"
     CDS             433..1554
                     /locus_tag="VFV87_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5104867.1"
                     /translation="MTTLLVAAIFPWLLIAVGMWLGYQLVRQNGRVLLRLESIEKTLG
                     SRAGKGAKKQREPAGLAIGTSAPDFELADLAGVRHRLSDFRERNALLIFFNPKCGYCT
                     KMAADLAALPPEGGAGRAMPLVVTTGDAEENRKLVEQFGIRCLVLLQEQMEVATQFLC
                     HGTPMGYRIDPAGRIASELAVGGEPLLKLADLKHAARSAANAGKKGATHKGNGKHPDP
                     SLAKSKLNRSGLKAGAVAPEFSLPKVGGGELSLADFRGRRVLLVFSDPNCGPCDELAS
                     LLQELHDERPDLAVVVISRRDAEATAAKAKSLGLTYPIVMQQQWEISKQYGMFATPIG
                     YLIDEQGILLSDVALGVEPILALAGQSQHQDARAFALST"
BASE COUNT          483 a          824 c          879 g          471 t
ORIGIN      
        1 tgcggtttga gggcggctcg ctgaacgaac gtgtcaacgt ggatccgaaa agcgtgacag
       61 ccgcagattt ccgtctccgc aaggacagcg ccggctaccg agccggcccc gacggccaag
      121 acctcggcgc cgacgtggac ctggtcggac ctggcgaagc ttacgagcgg tggaggaaga
      181 cgccggagta tcaagagtgg cttaaggaga cacgacagaa gaaggagtca ggagacagta
      241 gtcaggagtc agcaaatgga caatcaccaa actgatccct gactcctgat tcctgacccc
      301 cggaaaaaca agcggccggt gaggccctaa ccgtcaagca agctcctcac cggcccggca
      361 ccgccccggc gatcggtctg cgcgctggaa gcgatgcgtc tccatagtaa cgcaggccgc
      421 ttggagactt ccatgacgac gctcctggtc gcagctattt tcccttggct gctgattgct
      481 gtcggaatgt ggctgggata ccagctcgtc cgccagaacg gccgcgtcct gttgcggctg
      541 gagtcgatcg agaagacgct cggatcgcgc gccggcaagg gcgcgaagaa gcagcgcgag
      601 ccggctggcc tggcgattgg aacgtctgcg cccgatttcg agttggccga cctggctgga
      661 gtacggcaca ggctctcgga cttccgtgag cgcaatgcgc tgctgatctt cttcaatccg
      721 aagtgcggct attgcacgaa aatggccgcc gacctggccg ccctgccgcc ggaaggaggc
      781 gccggccggg ccatgccgct cgtcgtcacc accggcgatg cggaggagaa ccgcaagctc
      841 gtggagcagt ttggcattcg ctgcctcgtc ttgctccagg agcagatgga agttgccacg
      901 cagtttctct gccacgggac gccgatggga tatcgaatcg atcccgcagg gcggatcgcg
      961 agcgaattgg ccgtgggagg cgagccgctg ctgaagctgg cggacctgaa gcacgcggcg
     1021 cgctcggcgg ccaacgcggg aaagaagggt gcgacccaca aggggaacgg aaagcatccc
     1081 gatccgtcgc tggccaagag caagctgaac cgcagcggct tgaaggcggg agccgtcgcg
     1141 cccgagtttt cgctgccgaa ggtgggaggc ggcgagctgt cgctggccga tttccgcggc
     1201 cggcgcgtgc tcctggtctt ttccgatccg aactgcggcc cgtgcgacga actggcctcg
     1261 cttcttcagg agctccacga cgagcggccg gatctggcgg tggtggtcat cagccgccgc
     1321 gatgccgagg cgacagccgc caaggcgaag tcgctcggtc tcacgtatcc gatcgtgatg
     1381 cagcagcagt gggagatttc gaagcaatac gggatgttcg ccacgccgat cggctacctg
     1441 atcgatgagc agggcatcct gctgagcgat gttgccctag gagtcgagcc aatcctggcg
     1501 ctggccgggc aatcccaaca ccaggatgcc agggcgttcg cgttatcgac ctgaaatgca
     1561 cgttgcgatc tcgatccaaa tctcttttca cgaaaggcac caaccatgac tcatcactgg
     1621 gacgaattct cgaaatcgat ggctgcggac ccgttgccgc ggcggcagtc gctgcgcttg
     1681 ctgggagctg ctctggcggg cgctgtgctc ggtcctttcg ggctgcggac cgccttggct
     1741 gcgggagacc cttgcaaagc tttctgcaat caatgcccga agtcgcaaag gtctcaatgc
     1801 ctctccgcct gccaggcgtg taacggcaat accagccgcc tgggaggctc gtgcggaaac
     1861 tatgtctgct gctccggggc cgtttgcaac ggcgcatgca gcgacttgcg atccgacccg
     1921 aactgcgggg cctgcggaat cgactgccgc gacatcggcg aaacgtgttg cggaacctat
     1981 tgcgccgatc tggccagcga cttcgacaac tgcggcgcgt gcggccacca gtgcgcgccg
     2041 cccgggcctt acgaaaccgg cgcgtgcatc gacggcgcct gcctttacgc ctgcgtcgaa
     2101 ggcgctgtca agtgcaacgg agcctgcagc gatttggcta ccgatccgaa caactgcggc
     2161 gcgtgcggca atatctgccc ggcgtcggcg ccgcactgtg accagggcgt gtgcgtagca
     2221 actgacccca accccgacga ctgcggcggg tgcccggaag gccaggtatg cagcgacggt
     2281 tattgctgcg atccaggttg cactcccgac aatccgtcct acccgaattg cggcaccata
     2341 tgcggcgatc cctgcgcctc tttttggacc gggctgacgt ggtgtgaccc cgcttgcgtc
     2401 gacctcagct cggacccatt caactgcggc gcgtgcggca atgtctgcgg tggttcgacc
     2461 ccgtactgca gcgctggcgt gtgcattcgt ggggacgaac cgtgcccagt tggcttaacg
     2521 aggtgcaatg gcgggtgtac caacctggcc ttcgacacgg cgaattgcgg cgcgtgcggc
     2581 aatatctgca gcccgggcga gacctgctcg ggcggtgtgt gtcggttgcc gttctgagtt
     2641 ggcggactcc attcggc
//
LOCUS       DASUGB010000715         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1608744, whole genome shotgun sequence.
ACCESSION   DASUGB010000715 DASUGB010000000
VERSION     DASUGB010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608744"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..971)
                     /locus_tag="VFV87_13705"
     CDS             complement(111..971)
                     /locus_tag="VFV87_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5104868.1"
                     /translation="MDLSSTRIKTNGICLNVVQSGPVDGTLVILLHGFPEFWYGWRKQ
                     IPALANAGFRVWAPDQRGYNTSDKPPQVRDYRLDHLTDDVAGLIAGSGRQRVVVVGHD
                     WGGAVAWWLAANYPELVERLVILNVPHPLVMQRLIRTDLRQTLRSWYMFFMQIPWLPE
                     ALMRRGNWRGMARGLQSSSREGTFTETELELYRSAWSQRGAFTAMLNWYRAAFRYPPP
                     APRNERILPPTLILWGVRDKFIGREGALRSADLCDLGELVFYDRATHWLPLEEYPDVN
                     ERIIKFVRGA"
     gene            complement(1059..1628)
                     /locus_tag="VFV87_13710"
     CDS             complement(1059..1628)
                     /locus_tag="VFV87_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5104869.1"
                     /translation="MNRTRRLSGTRQLLLYDTGAALVTDFMRHVGGDFMRYGLLIDDY
                     AAGPKMLREAVAGMSREQALARPVAGKWSTLEVVCHLADFEIVYADRLKRVIAENEPT
                     MFGGDPDVFAKRLAYHQRDLAEELALIEAVRHSTARILRQLGEADFARIGRHSEAGPL
                     SLATLLERVTEHLPHHAQFIVEKRKALVM"
     gene            1778..2971
                     /locus_tag="VFV87_13715"
     CDS             1778..2971
                     /locus_tag="VFV87_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5104870.1"
                     /translation="MALSLPLLDAMAPAFAVESEAEIPRRMVAMETNMGILPQFFFPE
                     EAGAEYKASPYLERLAAHRENLTVFSGVSLPGVTGGHAAERCFLTGTPHPERGGFRNG
                     VSLDQLAAEQLGNRTRYPSLVLAISNENHTLSYTRSGAPIPAEKSPRQLFERLFFQGK
                     PEEIAANVEGLRQGRSLLDFVGEQSRRLGRQLSAGDRQRLDQYFSAVRDLEQRLHSAE
                     AWEHRDKPQVAGQPPDDITAAEEFARRTGQMLDVVKLALETDSTRIVSLFIDTTVIHS
                     ITHHGNRPEVLAELRGKEEAQFDALGTFLASLAASQEGGHSLLDRTMVLYGTCMGSAN
                     SHSNVNLPVLLAGGGFRHGQHLAFDTENNYPLSNLYVSMLQRLGLEADRFSTGTGTMR
                     GLELA"
     gene            2971..>4317
                     /locus_tag="VFV87_13720"
     CDS             2971..>4317
                     /locus_tag="VFV87_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEX5104871.1"
                     /translation="MRTGVGLSLVLVVLATPVARGDVPAFVAKYCADCHGSAEPDAGL
                     DLAALLLDLANREHFRRWVKVHDRASAGEMPPQDAPQPTAEERQTFVQSLHDALLAAE
                     KAQPEPSRLRRLTRAEYENTIRDLFDMPGIALAGNLPADGSAGGFDKHAEALDISHVN
                     LAKYMEAADHILNYAIATRPAAPSIQKRRISLVNRGGFVAHVVMNGDGVLLRDGQIEP
                     GFPPSGAQGHIDQGAHERMGSFDTGSSVGLFRHEDESFNPYFIEHVTIYPGVYRVRTS
                     LWSFRWDKGAVLPGRGTEAGRLSVVQLTGDGRGGQHPSYVLGYFNAPVGGAQEHELGV
                     WLNYNELIGFNTASLAPAANYYKKLRAMEFTGPGIVCDWLDIEGPLHEVWPPRSHKLL
                     FGDLPLVEFKADEHPGVRPPPRLKVRQLGAGQNRPDPEPGVWTAHSASPLEDADRLL"
BASE COUNT          775 a         1477 c         1375 g          690 t
ORIGIN      
        1 tccgggttat tgcttcgcaa aaacccggcc accctggggc aggagcaaga gcaggagcag
       61 gaagaagatt aggatcagga gcaggagcaa gagcaagatg aggattgcgc tcaggcccct
      121 cttacaaact tgatgatccg ctcgttcacg tccgggtact cctccagcgg cagccagtgc
      181 gtggcccggt cgtagaagac cagctcgccc aggtcgcaca gatcggcgct tcgcagcgcc
      241 ccttcgcggc cgatgaactt gtcgcggacg ccccacagga tcagcgtggg cggcagaatc
      301 cgctcgtttc gcggcgcggg gggcggatag cgaaacgccg cccggtacca gttcagcatc
      361 gcggtaaaag ccccgcgctg cgaccacgcg ctgcggtaca gctccagctc cgtttcggta
      421 aacgtcccct cgcggctgct ggattgcagc ccacgggcca tgccgcgcca gtttccgcgc
      481 cgcatcaagg cttccggcag ccagggtatc tgcatgaaga acatgtacca gcttcgcagc
      541 gtctgccgca gatcggtcct gatgagccgc tgcatcacca gcgggtgcgg cacattcaga
      601 atcaccagcc gctcgaccag ctccggataa ttcgccgcca gccaccaggc gacggccccg
      661 ccccaatcgt gaccgacgac gaccacccgc tggcggcccg agcctgcgat cagcccggcg
      721 acgtcgtcgg tcaaatgatc gagccggtaa tcccggacct gcggcggctt gtcgctggtg
      781 ttatagccgc gctggtcggg cgcccagacg cgaaacccgg cattcgccag cgccggaatc
      841 tgctttctcc agccgtacca gaactccgga aacccgtgca gcagaatcac cagcgtgcca
      901 tccaccggcc cggactggac gacattcagg caaatgccgt tggtcttgat ccgggtggag
      961 gagaggtcca tgcggggagg agagaggaca ggagacaggg gacaggagac aggggacaga
     1021 aaagacgtca caccactgac cgctcaccaa ctcaccactc acatcaccag ggcctttcgc
     1081 ttctccacga tgaactgcgc atgatgcggc aggtgctcgg tgacccgctc caggagcgtg
     1141 gccagcgaaa gtgggccagc ttcggaatgg cggccgatgc gggcgaagtc cgcctcgcca
     1201 agctgccgca ggatccgcgc ggtgctgtgg cggaccgcct cgatcagggc cagttcctcg
     1261 gccagatcgc gctggtgata cgccagcctt tttgcaaaca cgtccggatc gccgccgaac
     1321 atcgtcggct cgttctcggc aatcacccgc ttgaggcgat cggcatagac gatctcaaaa
     1381 tcggccaggt ggcacaccac ctccagcgtg gaccacttcc ccgccaccgg ccgcgccagg
     1441 gcctgctctc ggctcatccc ggccaccgct tcccgcaaca tcttcggccc ggcggcgtag
     1501 tcatcaatca gcaatccata gcgcatgaaa tcacccccga cgtggcgcat gaagtcggta
     1561 accaaagccg cgccggtatc atacagtaaa agctgccgcg tgccgctcag ccgtcgcgtt
     1621 cgattcaagg tgcgggttca atgccggatg gcagtgagat atgctgaaat gtcgatccgt
     1681 cgccgcaccc gttgcaggag ccgccatgca gtttgcagca cgaaccaggt atgcccgcgg
     1741 gggaatctca cggcggacgc tgctgcgcgg agctggcgtg gcgctgtcgc tcccgctgct
     1801 cgatgcgatg gcgcctgcat tcgctgtgga atcggaggcc gaaattcccc ggcggatggt
     1861 cgccatggaa accaacatgg gcatcctgcc ccagttcttc tttcccgagg aggccggagc
     1921 ggaatacaag gccagcccgt atctcgagcg gctggcggcc caccgggaga acctgacggt
     1981 cttttcgggc gtgagcctgc cgggcgtgac cggcggccat gccgcggagc gctgcttcct
     2041 gaccggcacg ccgcatccgg agcgcggcgg ctttcgcaac ggcgtctcgc tcgatcaact
     2101 tgcggccgaa cagctcggca accgcacgcg ctatccgtcg ctcgtcctgg ccatctcgaa
     2161 cgagaaccac acccttagct atacccgcag cggcgcgccg atcccggccg aaaagagccc
     2221 ccgccagctc ttcgagcggc tctttttcca gggcaagccg gaggaaatcg ccgccaacgt
     2281 ggagggcttg cggcaaggcc gcagcctgct cgacttcgtc ggcgagcagt cgcggcgcct
     2341 cggccgccag ctctcggccg gcgatcgcca gcggctggat cagtattttt ccgccgttcg
     2401 cgacctggag cagcgcctgc acagcgccga ggcgtgggag caccgcgaca agccgcaggt
     2461 cgccggccag cctccggacg acatcaccgc tgccgaggaa ttcgcccgcc gcacgggcca
     2521 gatgctcgac gtggtgaagc tggccctgga aaccgattcg acgcggatcg tctccctgtt
     2581 catcgacacg accgtgattc acagcattac gcaccacggc aaccggccgg aagtgctggc
     2641 cgagctgcgc ggcaaggaag aggcccagtt cgacgcgctg ggcaccttcc tggcttcgct
     2701 cgccgcaagc caggagggcg gccattcgct gctcgaccgc acgatggttc tctatggcac
     2761 ctgcatgggc agcgcgaatt cccatagcaa cgtgaacctg ccggtgctgc tggccggcgg
     2821 cggcttccgg cacgggcagc acctggcgtt cgacaccgag aacaactatc cgctctcgaa
     2881 cctgtatgtc tcgatgctgc agcggctggg gctggaagcc gaccgcttct cgaccggcac
     2941 gggcacgatg cgcggattgg agctggcgtg atgcgaacgg gcgtcggatt gagcctggtt
     3001 ttggtcgtgc tggcgacgcc cgtggcccgc ggcgatgtgc cggcattcgt cgccaaatac
     3061 tgcgccgatt gccacggctc ggctgagcct gatgcgggat tggatctggc cgcgctgctg
     3121 ctcgatctgg cgaaccgcga gcactttcgc cgctgggtga aagtccacga tcgcgccagc
     3181 gccggcgaaa tgcctcccca agacgccccc cagccgacag ccgaggagcg gcagacgttc
     3241 gtccaatcgc tgcacgatgc gctcctcgcc gcagaaaagg cccagccaga gccgtcgcgc
     3301 ctgcgtcggc tgacgcgggc cgaatatgaa aacacgatcc gcgacctgtt cgacatgccc
     3361 ggcatcgccc tggcgggcaa tttgcctgcg gatggctccg cgggcggctt cgacaagcat
     3421 gccgaggcgc tcgacatttc gcacgtcaac ctcgccaaat acatggaggc cgccgaccac
     3481 atcttgaact acgcgatcgc cacgcggccg gccgctccga gcatccagaa gcggcggatt
     3541 tcgctggtca accgcggcgg cttcgtggcc cacgtggtga tgaacggcga cggcgtgctg
     3601 ctgagggacg gccagatcga gcccggcttt cccccctccg gcgcccaggg gcacatcgac
     3661 cagggcgccc acgaacggat gggctccttc gacaccggca gcagcgtcgg cctcttccgc
     3721 cacgaagacg agtcgttcaa tccgtatttc atcgagcacg tcacgatcta ccccggcgtc
     3781 tatcgcgtgc ggacttcgct gtggagcttc cggtgggata agggcgcggt cctgccgggc
     3841 cgcgggaccg aggccggccg cctctcggtc gtgcagctca cgggcgacgg ccgcggcggc
     3901 cagcatccca gctatgtcct gggctacttc aacgcccctg tcggcggggc tcaggagcac
     3961 gagctgggag tctggctcaa ttacaacgag ctgatcggct tcaatacggc ttcgctcgcg
     4021 ccagcggcca actactacaa aaaactgcgg gcgatggagt tcaccgggcc gggcatcgtc
     4081 tgcgattggc tcgatatcga aggcccgctg cacgaagtct ggccgccgcg cagccacaag
     4141 ctgctgtttg gcgatctgcc gctcgtggaa ttcaaggccg acgagcatcc gggcgtccgc
     4201 ccgccgcccc ggctgaaggt gcgacagctc ggcgcggggc agaaccggcc cgatcccgag
     4261 cccggcgtgt ggaccgcgca tagcgcttcg cccctggagg atgccgaccg cctgctg
//
LOCUS       DASUGB010000716         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_497201, whole genome shotgun sequence.
ACCESSION   DASUGB010000716 DASUGB010000000
VERSION     DASUGB010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497201"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFV87_13725"
     CDS             <1..739
                     /locus_tag="VFV87_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5104872.1"
                     /translation="PAPRPAEGYPPGSFGDPHFDPHVAGWQDYYVHRWFAEPWFSHSD
                     PNDPYRHVGLGHPLTGTSWRNRPWFVGTFAGGVLMGDLIQDRVNASDTGFYGLRLGWD
                     FDHYWGAEGRFAFAEPQLSDVNGLPLDRDAQNYFGDVSLVCYPWGDSCWRPYLSAGVG
                     FQTLQLIDENGRYVSESPLAFPLGGGLKIFVSPWFTLRFDIVDNVAFGNDRVSLVNHF
                     ALMSGCELRFGGRPTSYMPWHGSTAYW"
     gene            854..1624
                     /locus_tag="VFV87_13730"
     CDS             854..1624
                     /locus_tag="VFV87_13730"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104873.1"
                     /translation="MQISRVTLALFAAAVAAIPLAGHAEQSPPAGAGAPSVANAIKTP
                     PKGFVALFNGKDLSGWRGGKTENPQKRLDLSPEERAKLDQESIEDIHKHWRVENGELV
                     NDGTGQYLTTDKDYADFELRLEYKTVAKADSGIYLRGHPQVQIWDTTKEGGKWDRNAD
                     KGSGGLFNNPKGGPGQLPLIHADKPFGEWNSFVITIVGDKVTVSLNDKLVVDQAPLAN
                     YFEKGEPLPASGPIQLQTHGGEIRWRNVFLREIPQSEK"
     gene            complement(1691..2632)
                     /locus_tag="VFV87_13735"
     CDS             complement(1691..2632)
                     /locus_tag="VFV87_13735"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX5104874.1"
                     /translation="MNEPVQLKTLTFGSRGAGPHLLITGGVHGDEFEPMVALRRLARS
                     LERTTLRGQVTLVPVVNEAAFARGDRVAEDGLDLARTCPGRPDGSITERTAHALSELI
                     RSADYYIDLHTGGTTLAVWPMTGYVLHSDPKILDQQRALARAFNLPVIWGTDPTLPGR
                     SLSVARDAGVPAIYAEYLGAGNVTAVGVDLYIEGCRNVLAHLGMLDQDAEADAVRWIL
                     EDPRTGSGHMQRCYPSPAAGYFEAAVFLGLPVESGQIIGSVVDPLGQIRHEIRATESG
                     LILVLRTFPSVKQGDSLAVILDTSFAIQSPRANSLTG"
     gene            complement(2629..4845)
                     /locus_tag="VFV87_13740"
     CDS             complement(2629..4845)
                     /locus_tag="VFV87_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5104875.1"
                     /translation="MKRIITGLLIVTFCAGSSPAEEGKLQAGAATSVITPPLGEKIVG
                     GFAPFPSTHIHDELHARCLVLDDGQTKLALVVCDLLGMHRIVSDEARRLIQEEAGIPK
                     ENVLISATHTHSASSALGQNRFSHEQTLDDYQKFVARRIADGVRCAVNNLRPAELGYG
                     SAEAPEHVNNRRWRLKPGTMPDNPFGEQDLVKMNPPAGSPNLVEPAGPTDPTVSFIAV
                     RGADGRPISVFTAYSLHYVGGVGNGHVSADYFAIYCDELARLLEAERLDPPFVALMAN
                     GTSGDINNINFREPRPRKEPYEQMRFVATDVAKKVHAALAGVKYKSNITLAARYREPE
                     IAWRHPSEERLAWAKKTLDAGPRVPGKVDLSVAYAERALAMADYPATTKVPLQVLRIG
                     EIGIGTMPCEVFCEIGIDFRNKCPLQPAFMVSLNHGYFGYLPTPRHHKLGGYETWLGT
                     NRLEVEASDKLLAALLEMAGECSPAAPQAVNRVDNAIREKCLQVLQAGLASNEFWPSM
                     HAAEALTYAGQTKEVLAALATKQAADDQERCGLAREAIRAGDRDKTGIMLAILAEEGS
                     IGRVHAAESLFKVGEMGDGQALRAAAAQDENLKLKLMAAAALTRGGNPSALALIRKYV
                     AHDDFEVRKVAVWILGQIGDKADIAPIQQSLAKEQDELARAYCVNALACLGDADAKKQ
                     LVANLSASNPAVKTYSADFAGHAKASAARERLIELLGDETLDVRMRAAQSLILLAK"
BASE COUNT          871 a         1585 c         1657 g          900 t
ORIGIN      
        1 ccccgctccg cggccagccg aaggctaccc tccgggcagc tttggcgatc cgcatttcga
       61 tcctcacgtc gccggctggc aggactacta cgtccaccgc tggtttgccg agccctggtt
      121 ctcgcacagc gatccgaacg atccgtaccg gcacgtgggc ctcggccatc cgctgaccgg
      181 cacaagttgg cggaaccggc cgtggttcgt gggcacgttc gccggcggcg tgctgatggg
      241 cgatttgatc caggaccgcg taaacgccag cgacacgggc ttctacggtc tgcggctggg
      301 atgggacttc gaccactact ggggcgcgga agggaggttt gcattcgcgg agccgcagct
      361 ttcggacgta aacggcttgc cgctcgaccg cgacgcgcag aattactttg gcgacgtgtc
      421 gctggtgtgc tatccatggg gcgattcgtg ctggcggccg tacctgtcgg caggcgtcgg
      481 ctttcagacg ctgcagctca tcgacgagaa cgggcgctac gtgagcgagt cgccgctcgc
      541 gttcccgctt ggcggcggcc tgaagatctt cgtgagcccg tggttcacgc tccgcttcga
      601 catcgtcgat aacgtcgcct tcggcaacga ccgcgtgagc ctggtcaatc acttcgccct
      661 gatgagcggc tgcgagctcc gcttcggcgg ccggccgacg agctacatgc cgtggcacgg
      721 gagtacggcg tattggtagc aacgtggggc agacattctt gtctgccgcg ggattgcggc
      781 cagtagtccc tcgctcacgc tgcgggttac gatggagcaa ccttccatcg ttcattgttt
      841 tgaggacaga tccatgcaga tctcacgagt tacgctggct ctcttcgccg ccgcggttgc
      901 agccattccg ttggcaggtc atgcagagca gtcgccgccg gccggcgccg gcgcgccctc
      961 agtcgccaac gcgatcaaga cgccgccgaa gggcttcgtt gccctcttca acggcaaaga
     1021 cctgagcggc tggcgcgggg gcaagaccga gaatccgcag aagcggctgg atctttcgcc
     1081 cgaagagcgg gccaagctcg atcaggagtc gattgaggac atccacaagc attggcgcgt
     1141 ggagaacggc gagctcgtca acgacggcac cggccagtat ctgacgaccg acaaggatta
     1201 cgccgatttt gagctgcggc tggagtataa gacggtcgcc aaggctgatt cggggattta
     1261 cctccgtggc catccgcagg tgcagatttg ggatacgacc aaggagggcg gcaagtggga
     1321 ccgcaacgcc gacaagggct ccggcggctt gttcaacaac cccaagggcg gtccgggaca
     1381 gcttccgctc attcacgccg acaagccgtt cggcgaatgg aattcgttcg tgatcacgat
     1441 cgtgggcgac aaagtgaccg tgtcgctcaa cgacaagctc gtcgtcgatc aggcgccgct
     1501 ggccaactat ttcgaaaaag gcgagcccct gccggcgagc gggccgattc aactgcaaac
     1561 gcacggcggc gagattcgtt ggcggaatgt cttcctgcgg gagattccgc aaagcgagaa
     1621 atagcgaagc ggcgtcgttt aacgcagagc cgcaggcgaa gccgtggcat atgcggcggg
     1681 gcgccttcgg ctacccggta agcgaattcg ccctcggcga ctggatggcg aagctggtgt
     1741 cgaggatcac ggccaggctg tcgccttgct tgacagaggg aaaagtgcgg aggacgagga
     1801 tcaagccgct ttcggtcgcg cggatctcgt gtcgaatttg accgagtgga tcgacgacgg
     1861 agccgatgat ctggcccgat tcgaccggca gccccaagaa gacggccgcc tcgaagtacc
     1921 cggcggctgg agaagggtag cagcgctgca tgtggccgct gccggtgcgc gggtcttcca
     1981 ggatccagcg gacggcgtcg gcctcggcat cctggtcaag catccccaga tgggccagga
     2041 cattgcggca gccttcgata tagaggtcaa ctcccacggc ggtgacgttg ccggcgccga
     2101 gatactccgc atagatcgcc ggcacgcccg catcgcgagc gaccgagagc gagcgtcccg
     2161 ggagcgtggg atcggtcccc caaatcaccg gcaggttgaa tgcgcgagcc agcgcccgct
     2221 gctggtcgag gattttcggg tcggaatgca ggacgtagcc ggtcatcggc cagacagcca
     2281 aggtcgttcc ccccgtgtgc aagtcgatgt agtaatcggc cgagcggatc agctcggaca
     2341 gggcatgcgc cgtccgttcc gtgatcgacc catccggccg accgggacag gtgcgggcca
     2401 gatcgaggcc atcctcggcg acgcgatccc cgcgggcgaa ggccgcttcg ttgacgacgg
     2461 gcacgagcgt gacctggccg cgaagcgtgg tcctttccaa cgatcgcgct aagcggcgca
     2521 gggcgaccat tggctcgaac tcatcgccat gcacaccacc cgtgatgagc agatgcggcc
     2581 cggcgccgcg gctgccgaac gtgagcgttt tcagttgaac cggctcgttc atttggccag
     2641 caagatcaac gattgcgcag cccgcatgcg cacgtcaagc gtttcgtcac ccagcaactc
     2701 gatcagccgc tcgcgagccg cagaggcctt cgcatgtccc gcgaaatcag ccgagtacgt
     2761 cttcactgca ggattgctgg ccgagaggtt cgcgacgagc tgctttttgg cgtccgcgtc
     2821 gcccaggcag gccagtgcgt tgacgcaata cgcgcgggcc agctcatcct gctccttggc
     2881 cagcgactgc tggatcggcg cgatgtcggc tttgtcgccg atctggccga gaatccagac
     2941 cgcgaccttg cggacctcga agtcatcgtg cgcgacgtac ttgcgaatca gcgccagcgc
     3001 cgaggggttg cccccgcgcg tcagcgcggc cgcggccatc agcttgagct tcaggttctc
     3061 atcctgagct gccgcggctc gcagcgcctg gccgtcgccc atttcgccga ccttgaacag
     3121 gctttcggct gcgtggacgc ggccaatcga gccctcttcg gccaggatcg ccagcatgat
     3181 gcctgttttg tcccgatctc ccgcgcgaat cgcctcgcgg gccaggccgc agcgctcctg
     3241 gtcgtcggca gcctgcttcg tggcgagggc cgccaggact tctttggtct ggccggcata
     3301 cgtcaatgct tcggcggcgt gcatggaggg ccagaactca ttggaggcga ggccggcttg
     3361 caggacttgc aggcactttt cgcgaatggc attgtccact cggttgactg cttgcggggc
     3421 ggcaggactg cattcgccgg ccatttccag caaggcggcc agcagcttgt cggaggcctc
     3481 cacttccagc cggttcgtgc ccagccacgt ctcgtagccc cccagcttgt gatgccgcgg
     3541 cgtcggcagg tagccgaaat agccgtgatt gagcgagacc atgaacgccg gctgcagcgg
     3601 gcacttattg cggaagtcga tgccgatctc gcagaagacc tcgcagggca tcgtgccgat
     3661 gccgatctcg ccgatccgca gcacctgcag cggaaccttg gtagtcgccg gatagtcggc
     3721 catcgccaag gcccgctcgg catacgcgac cgacaaatcg acctttcccg gaactctcgg
     3781 ccccgcgtcc agcgtcttct tcgcccaggc gaggcgctcc tcgctcggat gtcgccaggc
     3841 gatctccggc tcgcggtagc gggcggcgag cgtgatgttc gacttgtact tcacgcctgc
     3901 cagagccgcg tggaccttct tggccacatc ggtcgccaca aaacgcatct gctcataagg
     3961 ctctttccgc ggccgcggct cgcggaagtt gatgttgttg atgtcgccgc tggtgccgtt
     4021 ggccatcagg gcgacgaacg gcggatcgag ccgctcggct tccaacagcc gggccagctc
     4081 gtcgcaataa atggcaaagt aatcggccga gacatgccca ttaccgacgc cgcccacgta
     4141 gtgcagcgaa taagcggtaa agaccgagat cggccggcca tcggcgccgc ggaccgcgat
     4201 gaacgacacg gtcggatcgg tcggccccgc gggctctaca agattcgggc tgccggcggg
     4261 cgggttcatc ttcaccaggt cctgctcgcc gaacggattg tcgggcattg tccccggttt
     4321 caggcgccag cggcggttgt tgacgtgctc gggcgcctca gcagagccgt agcccagctc
     4381 ggccggccgc aggttgttga cggcacaccg cacgccgtcg gcaatccgcc gggcgacaaa
     4441 cttctgatag tcgtcgagcg tctgctcgtg gctgaagcga ttctggccga gggcgctgga
     4501 agccgagtgg gtgtgcgttg cggaaatgag cacgttctct ttgggaatgc ctgcctcttc
     4561 ctgaatcagc cgccgggcct cgtcgctgac gatgcggtgc atgccgagga ggtcacacac
     4621 gaccagcgcg agcttcgtct ggccgtcgtc gagcaccagg caccgggcgt gcagctcgtc
     4681 gtgaatgtgc gtggaaggaa acggcgcgaa gccgccgacg atcttctcgc ccaagggggg
     4741 cgtgatgacg ctcgtcgcgg cgccagcctg cagcttgcct tcttcggctg gggaggaacc
     4801 agcacagaat gtgacgatca agagaccagt gatgatgcgt ttcatgagat ggtccttggg
     4861 cgatggcaac taccgaatca gcgtagaggc gatggcggtg ccggacaact gctcgcggag
     4921 tcgccactgc tgggcctccg tcaggctggc ccgcggtgcg gcgcaagtgg cgtggtcgat
     4981 cagcccctgc cagacaagaa tctgcttcga ggc
//
LOCUS       DASUGB010000717         4950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1609041, whole genome shotgun sequence.
ACCESSION   DASUGB010000717 DASUGB010000000
VERSION     DASUGB010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609041"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFV87_13745"
     CDS             <1..948
                     /locus_tag="VFV87_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5104876.1"
                     /translation="AQRLVPSVAAALAIARALQCSVETLFGHPPAADGGTAFAWMPAS
                     FPCRYWAAELGGRILLYPLESGPQGGLLHDGIARGPGDLPANVKAAPSTLVLASCDPA
                     AGYLAEAYRRQGGFRMLVFSRTSTEALALVERRLVHVAGVHFSAGGSHEGNARALARH
                     HPATALSLVRVARWEEGLAVAPASRLRTATAAVRSKLRWIGRPAGAGARRCQDELLGK
                     NRAPRLMARDHRGIVEAIRSGWADVGVCVRLASEEGQLGFLPVCEEPYDLCFRSDAAD
                     DPRLRALVATLRSATFREIIDQLPGYRAADTGDLVTVES"
     gene            1002..1862
                     /locus_tag="VFV87_13750"
     CDS             1002..1862
                     /locus_tag="VFV87_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5104877.1"
                     /translation="MNHGSLKCREAVGMAVAVTALVASSQAAEPAQNQQVVRCAVIGG
                     LNETGFWPELAERFERATGHRAELVVTGPKPVIAEVFTSGQADVITMHASDTIVNLVA
                     DGLAENLQPWLRSDMVLVGPAADPAGIRGEKDAALALRKIIESKSKLLVHASLGAGEV
                     LHDLLAAGEMELDPEQTISLPSDKHRAMLQRAAAEGAYTLVGRIPLLNGKLDDGGMEI
                     MVQGDARLRRPYLVAVARRADDQLRLTAARDLVAFLRSVETQAWIAEYGRGKYDDQPV
                     FFPVAAPSKQ"
     gene            complement(1904..3154)
                     /locus_tag="VFV87_13755"
     CDS             complement(1904..3154)
                     /locus_tag="VFV87_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5104878.1"
                     /translation="MPIRRRAWLTREGWYYSAVLAFIVAGAILKSVNLLVVLAGVMIA
                     PLIINWRMVMASLSGLTIRRKLPEQICAGEPLTVEVVVANPRRWMSSWMTTVEDWVER
                     SGSGKQEAGVGSQESEFSRRGRWTWRGLIPRRMFRRQGVRGEAIVTHVPAGGTAIGTY
                     RLTLHRRGRYRFGPLRVSTRFPLGLVSGRFTQPADGELIVAPHIGRMTSQWLELIEAE
                     LAGDQQRHPQRGISEGDYYGLRPWQSGDSMRWVHWRTTAKLGRPIVRQFERRRNRDVA
                     IVLDPWRPEPFDERDDGLLELAISLAATAITDLASRGQSRLTFAIAGQRTQCWTGPAS
                     PMFCREVLAQLAEVQPTASGRLAVALARASEEAPYGTRLLVISPRSAEAAGKSSGSLE
                     LPYAPEDLSWIDVSSDQLDSLYVL"
     gene            complement(3166..4062)
                     /locus_tag="VFV87_13760"
     CDS             complement(3166..4062)
                     /locus_tag="VFV87_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5104879.1"
                     /translation="MGQVVLGKADVVRWCLVALLSGEHVLLEDVPGVGKTLVAKALAR
                     SVAGDFCRLQFTPDLLPSDILGSSVFDSKQGQFIFHRGPIFSNIVLADEINRAPPRTQ
                     SALLEAMSEGQVSVDGQTFTLPRPFLVIATQNPFEFEGTYALPESQLDRFLLRLSMGY
                     PGREDERRVLATHRNGEPVQDLEPVVECEQVVALQNSVRAVSFEESVSEYLLDIVHRT
                     RDSDELRVGVSTRGALSLYRAAQALALVEGRAFVVPDDVKRLAPLVFAHRVILKGFNR
                     TGLREAADGIIRRLVEQTPVPT"
     gene            4433..>4950
                     /locus_tag="VFV87_13765"
     CDS             4433..>4950
                     /locus_tag="VFV87_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104880.1"
                     /translation="MRQILDLGKKALQRVNARVAILIVVVAAGGVAVWQGFRYMAAQK
                     VDGPTKQIVTGVPGEASLDPTVRQASATVSDHPTGASPYQLTGYGQDASGSGSAANPY
                     APSADDPVGGAAGGGYGGGFSEGSSRAGYGGAARPATSLPVDPTTADAGVPDNPYRAA
                     GNGDSLYSPSSDS"
BASE COUNT          890 a         1634 c         1553 g          873 t
ORIGIN      
        1 gcgcagcgcc tcgtgccttc cgtggccgct gcgctcgcca ttgcccgcgc gctgcaatgc
       61 tccgtcgaaa cgctctttgg ccatccgccg gctgccgatg gcgggacggc cttcgcctgg
      121 atgcctgcgt cgtttccttg ccggtactgg gcggcggaac tcgggggacg aatcctcctc
      181 tatcccctgg aaagcggccc gcaaggcgga ttgctgcacg atggcatcgc ccgggggccg
      241 ggcgatctgc cggcaaatgt gaaagcagcc ccctcgacac tcgtcctcgc ctcctgtgat
      301 cccgctgcgg gctatttggc cgaggcgtat cgccggcaag gaggatttcg aatgctggta
      361 ttcagccgaa cgagcacaga ggctctagcg ctcgtggagc gaagactggt gcatgtcgcc
      421 ggcgttcact tctcagccgg cggcagtcac gaagggaatg cccgcgcgct cgcaaggcac
      481 catccggcga ccgccctctc cctcgtacgt gtcgcgaggt gggaggaagg tctggccgtc
      541 gcgcccgcga gccgactgcg aacggccacc gccgcggtcc gcagcaagct gcgctggatc
      601 gggcgaccgg ccggggccgg cgcgcggcgc tgccaggacg aactgctggg gaagaaccgc
      661 gctccgcggc tgatggctcg cgatcatcgg ggaatcgtcg aggccattcg cagcggctgg
      721 gccgatgtcg gcgtttgcgt gcgactcgcg agcgaagagg ggcaactcgg ttttctgccc
      781 gtctgtgaag agccctacga tttgtgcttc cggagcgacg cggccgacga tccgcgtctg
      841 cgagccctgg tggcgacttt gcgatcggcc accttccggg aaatcatcga tcagctcccc
      901 ggttatcgtg ccgcggacac cggcgatctg gtcacggtcg aatcctgatc tcctgtagcc
      961 gcatctacct cctgaatcca tttgtacgca tgcaattgca catgaaccac ggttcgctga
     1021 agtgccggga agcggtcgga atggcggtcg ctgtcacggc gttggttgct tcctcgcaag
     1081 ctgcagagcc agcgcagaac cagcaggtcg tccgctgcgc cgtcatcggc ggactgaacg
     1141 agactggctt ctggccggag ttggccgagc ggttcgagcg ggccactggg caccgtgcag
     1201 aattggtcgt tactggcccc aagccggtga ttgccgaagt gttcaccagc ggccaggccg
     1261 acgtcatcac gatgcacgcc agcgacacga tcgtcaatct ggttgccgac ggcctcgcgg
     1321 aaaacctgca gccatggctg agatcggaca tggtcctcgt cggcccggcg gcggatccgg
     1381 cgggcatccg cggcgaaaag gacgcggcac tcgccctgcg gaagatcatt gagtcgaagt
     1441 ccaaactgct ggtccatgcc agcctgggtg cgggcgaagt gctccacgat ctcttggccg
     1501 ccggcgagat ggagctcgat cccgagcaaa ccatctcgct ccccagcgac aaacaccggg
     1561 cgatgctcca gcgcgccgct gcggaaggcg cttacaccct ggtcggccgc attccgctgc
     1621 tcaacggaaa gctggatgac ggcggcatgg aaatcatggt gcagggcgac gcccgcctgc
     1681 ggcgaccata cctggtggcc gtcgcgcgcc gcgcagatga ccagctgcgc ctaactgcgg
     1741 cccgcgatct ggttgcgttc ctgcgctcgg tcgagacgca agcatggatc gccgaatacg
     1801 gccgcgggaa atacgacgat cagccggtgt tctttccagt tgctgcgccc tcgaagcaat
     1861 aaacgtactg cggtcgctac agctcgctcc gctgttgctt cggtcacaat acgtacagcg
     1921 aatcaagctg atcgctggag acgtcgatcc agctcagatc ctctggggca tagggcagct
     1981 caagcgagcc cgacgacttt ccggctgctt cggcggatcg ggggctgatc accagcagcc
     2041 gcgtgccgta aggcgcttct tcgctggccc gcgcgagcgc gaccgccaga cgtccgctgg
     2101 cggtcggctg gacttcggca agctgggcca gcacttcccg gcaaaacatg ggtgaagccg
     2161 ggccagtcca gcactgcgtc cgttgcccgg cgatggcgaa cgtcagccgg gattgcccgc
     2221 ggctggccag atcggtgatg gccgtggctg ccaggctgat ggctaattcc agcagcccat
     2281 cgtctcgctc atcaaacggt tcgggcctcc aggggtcgag gacgatggca acgtcgcggt
     2341 tgcgtcgccg ttcgaactgt cgcacgatcg gccggccaag cttcgcggtc gtccgccaat
     2401 ggacccatcg catgctgtcg ccgctttgcc aggggcgcaa gccatagtag tcgccttccg
     2461 atattccccg ctggggatgg cgctgctggt cgcccgcgag ttccgcctcg atcaattcca
     2521 gccactggga ggtcattcgg ccaatgtgcg gggcaacgat cagctcgcca tctgccggct
     2581 gtgtgaagcg gccgctcacc aggcccaacg gaaagcgcgt cgaaacgcga agtggaccaa
     2641 accgatagcg accccggcga tgcagcgtga ggcgatacgt gccgatggcg gtcccgcccg
     2701 ccggcacatg cgtgacgatc gcctctccgc ggacgccttg ccgtcgaaac atccgcctcg
     2761 gaatcaagcc tctccacgtc caccgtcccc gcctactgaa ttccgactcc tgactcccaa
     2821 ctcccgcctc ctgcttcccg ctgcctgacc gctcgaccca atcctccacg gtcgtcatcc
     2881 aggagctcat ccagcgtcgc ggattggcga cgacgacttc caccgtaagc ggctcgccgg
     2941 cgcaaatctg ttcgggcaat tttcgccgaa tcgtaagccc cgacagactg gccatcacca
     3001 ttcgccagtt gatgatcaag ggcgcgatca tcacgcccgc gagcacgacc aataggttca
     3061 cgctcttgag aatcgcgccg gcaacgatga acgccagcac ggcgctgtag taccacccct
     3121 cacgagtcag ccaggctcgg cgtcggatgg gcatggagca gtgatctagg tcggcacggg
     3181 tgtttgctcg accaatcggc gaatgatgcc gtcggcggct tcgcgcaggc cggtccggtt
     3241 gaagcctttg aggatgacgc ggtgggcgaa gaccagcggt gcaagccttt tgacatcgtc
     3301 gggaacgacg aacgcccgcc cctcgaccaa agccagcgcc tgagccgccc ggtagagact
     3361 aagcgccccg cgcgtcgaga cgcccacgcg caactcgtcg gagtcgcgcg tgcgatgcac
     3421 aatatcaagc agatattcgc tcaccgattc ctcgaacgag accgcacgaa ccgaattctg
     3481 cagtgccacg acctgctcgc actcgacgac cggctccagg tcttgcaccg gctcgccgtt
     3541 gcgatgcgtg gccaagacgc gccgctcgtc ctcacggccg ggataaccca tcgacaggcg
     3601 aagcaggaac cggtcgagct ggctttcggg gagggcgtac gttccctcga attcgaaggg
     3661 attctgcgtc gcgatcacca gaaacggccg cggcagcgtg aaggtctggc cgtcgaccga
     3721 tacctgacct tcgctcatcg cttccaggag ggcagactgc gtcctaggcg gcgcgcggtt
     3781 gatttcgtcg gcaagaacaa tgttcgagaa gatcggcccg cgatgaaaaa tgaactggcc
     3841 ctgtttgctg tcaaagacgg aactgccgag gatgtcgctg ggaagcaggt cgggcgtgaa
     3901 ctgcaggcgg caaaaatcac cagcaacgct gcgggccaag gccttggcca caagcgtctt
     3961 cccgacgccg ggcacgtcct ccaagagcac gtgctcacca gacagaagtg ccaccagaca
     4021 ccagcggacg acatccgcct ttcccaagac aacctgcccc atgttctctt cgagccgtcc
     4081 gacaagttga tagagccggt ccggttcaag catctcggcg ctgcgagctt cagcggcaga
     4141 aaggtccatg gtcgattcac cgcgttgcgg catggtcccc aagctgtcga ttcattcgat
     4201 ggaaagcatc tccataatac tgcctcaccc gcgggttgtc tgcttgccag actgcacaac
     4261 ttgcacaatc tgccggaaaa tttgctggtc tgccgacagc ccacaaacca caggacttcg
     4321 ctggcttacc ccgcttgtct aagcgaattg cccttccgca gcctgtgacg gtttggtaat
     4381 cacccgcgat tctttcccgc cattttctca ggcctgcgac ggggacgcga tcatgcggca
     4441 aattctcgac ctcggcaaaa aagcccttca gcgcgtgaac gcccgcgtgg cgatcctgat
     4501 cgtcgttgtg gcggccggcg gcgtcgccgt ctggcaaggc tttcgctaca tggccgctca
     4561 gaaagtggac ggtccgacga agcaaatcgt aacgggtgtt ccgggcgagg cttcgctgga
     4621 tcccacggtg cggcaagctt cggcgaccgt gagtgatcac ccgactggcg ccagcccgta
     4681 tcagctcact gggtacgggc aggacgcaag tggaagcgga tcagccgcga atccgtatgc
     4741 gcccagcgcc gatgaccctg tgggcggagc ggccggcggc ggatatggcg gtggcttttc
     4801 ggagggcagc tcgcgtgcgg gatatggcgg cgccgcacgc cctgcgactt cgctgccggt
     4861 cgatcccaca actgccgacg ctggggttcc cgacaatcct taccgcgctg ctggcaatgg
     4921 cgactcgctc tacagcccct ccagcgattc
//
LOCUS       DASUGB010000718         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1747508, whole genome shotgun sequence.
ACCESSION   DASUGB010000718 DASUGB010000000
VERSION     DASUGB010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747508"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFV87_13770"
     CDS             complement(<1..330)
                     /locus_tag="VFV87_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEX5104881.1"
                     /translation="MESSARDFLVRLLDTPSPSGYERPIQEVVRAYVREFADRVTTDL
                     HGNVIACKNPSAKLRVMFAGHCDQIGMLITHFNDNGVLHAQTIGGWDAQQLIGQRMTI
                     WTQSGPLP"
     gene            679..2250
                     /gene="cimA"
                     /locus_tag="VFV87_13775"
     CDS             679..2250
                     /gene="cimA"
                     /locus_tag="VFV87_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX5104882.1"
                     /translation="MRSIQIYDTTLRDGTQGEGVSLSLQDKLQIAQRLDEIGVDYIEG
                     GYPASNVKDAEFFQRIRELKLGHAKVCAFGMTRRRGMKPADDIGLQALVQAQTPVVTI
                     VGKTWDFHVTEVLRVSLQENLDMIRETVEYLASQSREVFYDAEHLFDGWKANPEYAAE
                     TLLAAARGGAKLVILCDTNGGSLPEVVAERTRAAIQLLEPLGVPVGIHTHNDGELAVA
                     NSLAAVDAGAVQVQGTINGFGERCGNADLISVMANLGIKKGYKVLGGRPLTHLTELSR
                     FLYETANVNHRNHQPFVGQSAFAHKGGMHVHAIARATSTYEHMDPELVGNERRILVSE
                     LSGRSNITALTAKHKMRDDKALMDAILKQVVDLEAQGWQFEAADASFDMLVRRAAGTF
                     QPHFERVKYHVGVSSEGDGVPVTEATIKLCVNGQDRHEVAEGDGPVNALDAAMRKALN
                     GDFPGLKEMRLIDYKVRVVNSEAGTAARIRVIIESSDHHDVWGTVGVSENIIEASWLA
                     LVDAIEYKLYKDENL"
     gene            2304..3089
                     /locus_tag="VFV87_13780"
     CDS             2304..3089
                     /locus_tag="VFV87_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX5104883.1"
                     /translation="MPSRFPGMDPFIEMDQWQEFHVNMVVEIQRQLVPLLSPKYVARV
                     ERRVYTEHLFDEVEIIQPDVEIVRASAKRSKKEPAGAAAAVAEVEPRLYAVPVPHQRS
                     EPFVKIIDRGTGQVVTLIEFLSPGNKARGSDGFRAYYEKREEVLQSSIHLVEIDLLRG
                     GTRLPTIDPLQETTDYCVFINRAPKRHHAEVVEWPLRHPLPAFPLPLGAADRDLRFDL
                     QRTFASVFDGAGYERFIDYRVTLKPPLHKDDMGWVRKLISKHD"
     gene            3144..>5116
                     /locus_tag="VFV87_13785"
     CDS             3144..>5116
                     /locus_tag="VFV87_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5104884.1"
                     /translation="MSKAFSPESLPNRFEFAEAQARLYAEWESHGFFHAEPPQPGEPD
                     PSKGPYCIVIPPPNVTGALHLGHALNNTLQDIQIRLHRMQGYNTLWMPGTDHAGIATQ
                     AVVERRLREEEGKTRHDLGREKLVERIWQWKEQYEARILSQLKQMGCSCDWARTRFTL
                     DEGCSRAVRQTFFDLFSKGLIYRGKRLVNWDTFLQTAVSDDEVFQETVKGKFWHFKYP
                     VVPTTPTRSVSEGEPTHVEIATTRPETMLGDTAVAVHPDPAAALDRAEAELRERLEKA
                     PAKEKDAIQAQIDNIAARRREMLPLLIKLRDMAKAGRKVMLPLLNREIPLICDEWAKP
                     ELGTGAVKITPAHDPNDYEVGKRQNLPMINILNADGTLNENAGPYKGLTIPKARDRVV
                     ADLDKLGLLGDVEDREIELPHSDRSKTPIEPYLADQWFVRMGDREQGTGDKGQGTGIV
                     ARSASEGNAASTRDFAGLAQMAMDAVSDGRVNIVPPRYSKSYLDWLSEKRDWPVSRQL
                     WWGHRIAVWSFILKVEIYRSDAVSREESLQRVVLEFEQAIADFATACGLAGHFCVGPS
                     ETSRTIYFVCARSEAAIEQIECFYRRHHRARMVAPGEPDPPFEPDLQGNDPRYEPDQR
                     IRKLYAHMDDQDEGVLDTWFSSALWPHSTLGWP"
BASE COUNT         1065 a         1575 c         1602 g          874 t
ORIGIN      
        1 cgggagcggg ccgctctggg tccagatcgt catccgctgg ccgatgagct gctgggcgtc
       61 ccagccgccg atggtctggg cgtgcaggac accgttgtcg ttgaagtgcg tgatcagcat
      121 gccgatctgg tcgcagtggc cggcaaacat cacccgcagc ttggcgctcg ggttcttgca
      181 ggcgatcacg ttgccgtgca ggtcggtcgt cacccggtcg gcgaactcgc ggacgtacgc
      241 gcgaaccacc tcctgaatcg gccgctcata gccggaggga gaaggagtat cgagcaggcg
      301 gaccaggaaa tcgcgggcgg aggattccat gggggagtgc agcgttcggg agtcgtagag
      361 gtcgttgaca gtgtcgaatt ctgaacgtag ggtttattcc agcaacattt tccgctttcg
      421 gctatggccc aggtttccta taacacgatt tcccggcggc acgctcaggc gggcgtagtc
      481 ggcgtcgtga ttattatcgg gaccctccgg ggctaacggg ccgcaagcgg tagcaaacca
      541 actacaagcc agccctgaag cccgagcggg tttcagggct tttttgttga cgcctgtagg
      601 gaacgccctc cgtggcgttc cgagacccgc cgccgcggat cgccacagag ggcgatccct
      661 acagaaatgg acctagccat gcgcagcatc caaatctacg acaccaccct ccgcgacggc
      721 acgcagggcg aaggcgtcag cctttcgctg caggacaagc tgcaaatcgc gcagcggctg
      781 gatgaaatcg gcgtcgatta catcgaaggg ggctatccgg ccagcaacgt caaggacgcc
      841 gagttctttc agcggattcg cgagctgaag ctcggccatg cgaaggtctg cgcgttcggc
      901 atgacccgcc gccgcggcat gaagccggcg gacgacatcg gccttcaggc gctcgtgcag
      961 gcacaaacgc cggtcgtcac catcgtcggc aagacgtggg actttcacgt caccgaggtc
     1021 ctgcgcgtca gcctgcagga aaacctcgac atgatccgcg agacggtcga gtatctcgcc
     1081 agccagagcc gcgaggtctt ttacgacgcc gagcacctgt tcgacggctg gaaggccaac
     1141 ccggaatatg cggccgaaac gctgctggca gcggcacgag gcggggcgaa gctcgtcatt
     1201 ctttgcgaca ccaacggcgg ctcgctgccg gaggtcgtcg ccgagcggac ccgcgcggcg
     1261 attcaactcc tggagccgct cggcgtaccg gtcggcatcc atacgcacaa cgacggagaa
     1321 ctcgccgtgg ccaactcgct cgccgccgtc gatgccggcg cggtgcaagt gcaaggcacg
     1381 atcaacggct ttggcgagcg ctgcggcaac gcggacctga tcagcgtcat ggccaatctg
     1441 gggatcaaga agggctacaa ggtgctcggc ggccggccgc tgacgcacct gaccgagctg
     1501 tcgcggttcc tgtatgagac agccaacgtg aaccatcgca atcaccagcc gttcgtcggg
     1561 caaagcgcct tcgcccacaa gggcgggatg cacgtccacg ccattgccag ggccacgagc
     1621 acttacgagc acatggaccc ggaactcgtc ggcaacgagc ggcggattct cgtcagcgag
     1681 ctgtccggcc gatcgaacat caccgccctg accgccaagc acaaaatgcg cgacgacaag
     1741 gccctgatgg acgcgatcct caagcaggtc gtcgatctgg aggcccaggg ttggcagttc
     1801 gaggcggcgg atgcgtcgtt tgacatgctc gtccgccgcg ctgccggcac gttccagccg
     1861 cactttgagc gggtgaaata tcatgtcggc gtcagctccg agggcgacgg cgttccggtg
     1921 accgaggcga cgatcaagct ctgcgtgaac ggccaggacc ggcacgaagt ggccgaggga
     1981 gacggtcccg tcaacgccct cgacgccgcc atgcgcaagg ccctcaatgg cgactttccc
     2041 ggcctcaaag agatgcggct gatcgactat aaagtccgcg tcgtcaacag cgaagcgggc
     2101 acggctgccc gcatccgcgt gatcatcgag tcgagcgacc accacgacgt ctggggcacg
     2161 gtcggcgtca gcgagaacat catcgaagcg agctggctgg cgctggtcga tgcgattgag
     2221 tacaagctct ataaggatga gaacctctaa tcatcgggac tgagacgacg cccgctgcag
     2281 ccggacgatc tggagaaatc aatatgccct cgcgatttcc agggatggac ccatttatcg
     2341 agatggatca gtggcaagag ttccacgtca acatggtcgt tgagattcag cggcagctcg
     2401 ttccgttgct gtcgcccaag tatgtggcac gcgtggaacg tcgggtctat acggagcatc
     2461 tcttcgatga ggtggagatc attcagccgg acgtcgaaat cgtgcgggcg tcggccaagc
     2521 gctcgaagaa agagccggcc ggcgctgccg cggcagtagc ggaagtcgag cctcggctat
     2581 atgccgtccc ggtgccgcat cagcgcagcg agccgttcgt gaaaatcatc gaccgtggaa
     2641 cgggacaggt cgtgacgttg attgagtttc tgtcgccggg caataaagcc cgcggcagcg
     2701 atggatttcg cgcctactac gaaaaacgcg aagaagtcct gcaaagttcg attcatctgg
     2761 tcgagatcga cctgctgcgg ggcggaacgc ggctgccgac catcgatcca ctgcaggaaa
     2821 cgacggatta ttgcgtcttc atcaatcgag cgccgaaacg ccaccatgcg gaggtcgttg
     2881 aatggccgct gcgccacccc ttgcctgcgt ttcccttgcc gcttggcgcg gccgatcgtg
     2941 atctgcgatt tgacctccaa cgaacattcg ccagcgtctt cgacggcgcc ggctacgaac
     3001 ggttcatcga ttacagggtc acgctcaaac caccgctcca caaggatgac atgggctggg
     3061 tgcgcaaact gatctcgaaa catgattgat tcgtccgatc cgctccaact ggcgcgaatc
     3121 aattcacctt tcctagctat ccaatgtcca aggctttctc ccccgaatcc ctgcccaacc
     3181 gctttgaatt cgccgaggcc caggcccgct tatatgcgga gtgggaatcc cacgggttct
     3241 tccacgccga gccgccgcag cccggcgagc cggacccgtc gaaggggccg tattgcattg
     3301 tcattccgcc gccgaatgtg accggggcgc tgcacctggg gcatgcgctc aacaacacgc
     3361 tgcaggacat ccagatccgc ctgcaccgca tgcagggtta caacacgctc tggatgccgg
     3421 gcaccgatca cgcgggcatc gccacgcaag cggtcgtcga acgccggctg cgggaggaag
     3481 agggcaaaac gcggcacgac ctggggcgcg agaagctcgt cgagcgcatc tggcagtgga
     3541 aggagcaata cgaagcccgc atcctcagcc agctcaaaca gatgggctgc tcgtgcgact
     3601 gggcccgcac gcggttcacg ctcgacgaag gctgctcgcg ggccgtgcgg cagaccttct
     3661 tcgacctctt cagcaaaggc ctcatctacc gcggcaagcg gctggtgaac tgggacacgt
     3721 tcctgcaaac cgccgtcagc gacgacgagg tctttcagga gacggtgaag gggaagtttt
     3781 ggcatttcaa gtatcccgtg gtgcccacca caccaacccg aagcgtaagc gagggcgaac
     3841 cgacgcacgt cgaaatcgcc acaacccgtc ccgaaacgat gctcggcgac acggccgttg
     3901 ccgtacaccc ggaccccgcc gccgcactcg accgggccga agccgagctg cgcgagcgtc
     3961 tggagaaagc cccggccaag gagaaggacg cgatccaggc gcaaatcgac aacatcgccg
     4021 cccggcgcag ggagatgctg ccgctgctga tcaagctgcg cgacatggcg aaggcaggcc
     4081 gcaaggtcat gctgccgctc ttgaatcgcg aaatcccact catctgcgac gaatgggcca
     4141 agccggagtt gggaactgga gcggtgaaga tcacgccggc ccacgatccg aacgactacg
     4201 aagtcggcaa gcgtcagaac ctgccgatga tcaacatcct gaacgccgac ggcacgctca
     4261 acgaaaacgc cgggccgtac aaaggcctca caattcccaa ggcccgcgac cgcgtggtcg
     4321 ccgacctcga caaactcggc ctcctgggcg acgtggaaga ccgcgagatc gagctgccgc
     4381 actccgaccg cagcaagacg ccgatcgagc cgtatttggc ggatcagtgg tttgtgcgga
     4441 tgggggacag ggaacagggg acaggggaca agggacaggg gactggcata gtagcccgta
     4501 gcgcaagcga gggtaatgcg gcctccactc gggactttgc tggactcgcc caaatggcga
     4561 tggatgccgt atcagacggc cgcgtgaaca tcgtgccgcc gcggtattcc aagagctatc
     4621 tcgattggct ctccgaaaag cgcgactggc ccgtcagccg gcagctttgg tgggggcatc
     4681 ggattgccgt gtggtcattc atcttgaagg ttgaaatcta tcgcagcgac gcggtttctc
     4741 gtgaggaaag tcttcaacgt gtagtcctag aatttgaaca ggcaatcgcc gactttgcga
     4801 cggcgtgtgg gctcgcagga catttctgcg tcggaccaag tgaaactagc aggaccatct
     4861 atttcgtatg tgcccgatca gaggcggcca ttgagcagat cgaatgcttc taccggcgac
     4921 atcatcgtgc gcgaatggta gctccgggcg aacccgatcc gccttttgaa ccggaccttc
     4981 aaggcaatga tccgagatat gaacctgatc aacgtattcg aaagctctat gctcacatgg
     5041 acgatcagga tgaaggcgtc ctcgacactt ggttctcctc cgccctctgg ccgcatagca
     5101 cgctgggatg gccgga
//
LOCUS       DASUGB010000719         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1609058, whole genome shotgun sequence.
ACCESSION   DASUGB010000719 DASUGB010000000
VERSION     DASUGB010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609058"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFV87_13790"
     CDS             <1..467
                     /locus_tag="VFV87_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104885.1"
                     /translation="DQLQPFGVQVVRQAEREVWLTKRNLDGSLEIEYEYDPERAPGAG
                     EDISLMSSASIEPSESSARESFGMTLAAYRLGFGIYDVQARELPHAMPGADQSHFALI
                     VKDGNPVGNMVVVQQGKRVHGLLLIGLYFDDPQELDDLLRPTIDRSAGIPLR"
     gene            485..1030
                     /locus_tag="VFV87_13795"
     CDS             485..1030
                     /locus_tag="VFV87_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104886.1"
                     /translation="MGGWRNILNLWNPTRKWRSDPSRLLVLDLDQHQLCGVGIGDSVA
                     TLAFLGPATGWSGRLQFPNHGLAIVEEGTVAELHFFFGHTGEPAGGTFRGEIRHLGAA
                     IPPLTSHTENDIVGRFGPPYWRDADGSETILFYEFPQREWQWEFGLDGRLKCLVIGLP
                     ILADQAQRDAYQVTRPWPPWS"
     gene            complement(1438..2139)
                     /locus_tag="VFV87_13800"
     CDS             complement(1438..2139)
                     /locus_tag="VFV87_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104887.1"
                     /translation="MHCHEFETRLNHILDDRGQPRADAQLAAHAETCDDCRQLLLGQE
                     MLLAGLRRFSPPPVSSGFSRRVVAQAASAERLSARAMPASSSRKIVWALATVLSTAAV
                     MLLIVSLIWQARRGPMPRPGDQVAKQSTPSPGTASQPRRSRPSGLAMTQPGVSQPNWI
                     RGYRGTIDNLAVTLPETVQRLDEVEHYAPGIRPIRISLGVLLDALWRAIPGSHGDEAR
                     PTRTSCLPVDLLRIA"
     gene            complement(2150..>2424)
                     /locus_tag="VFV87_13805"
     CDS             complement(2150..>2424)
                     /locus_tag="VFV87_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5104888.1"
                     /translation="IPDKEPDHRPAQHLAEEVQLALATLRDEYRQAFVLFHEQELCYA
                     DIAAAMEVPLGTIKTWVHRARRELIDCLRKRGAIPEHVALGNVAET"
BASE COUNT          446 a          686 c          867 g          425 t
ORIGIN      
        1 cagaccagtt gcagccgttt ggcgtgcagg tcgtgcggca ggcggagcgc gaggtgtggc
       61 tgacgaagcg gaacctcgac ggcagcctgg agatcgagta cgaatacgac ccggagcggg
      121 cgccgggtgc gggcgaggac atcagcctga tgtcgtcagc gagcatcgag ccgagcgagt
      181 cgtcggcccg cgagtcgttt ggcatgacgc tggccgcgta ccgcctgggt ttcgggattt
      241 acgacgtgca ggcccgcgag ctgccgcacg cgatgccggg ggccgaccag agccacttcg
      301 ccctgatcgt caaggatggg aatcccgtcg gcaacatggt agtggtccag caagggaagc
      361 gcgtccacgg cctgctcttg atcggcttgt acttcgacga tccgcaagag ctcgacgacc
      421 tcctgcggcc gacgatcgac cgctcggccg gcatcccgct gcggtaagat gcgaccaggg
      481 cgaaatgggt ggctggcgca acatcttgaa tctctggaat cccacgcgga aatggcgcag
      541 cgatccgagt cgattgctcg ttctcgactt ggatcagcac cagttgtgcg gcgtcggcat
      601 cggtgacagc gtggcaaccc tcgcgtttct gggcccggca accggctggt caggacgact
      661 gcagtttccc aatcatggac tggcgattgt cgaggagggc accgttgcag agctacattt
      721 ctttttcggc cacacgggcg agccggcggg cgggacgttc cgcggcgaaa tccgccacct
      781 gggggccgcg atcccgccgc ttacgtcgca tacggaaaac gacatcgtcg ggcgatttgg
      841 accgccgtat tggcgtgacg ctgacggtag cgagacgatc ttgttctacg aattcccgca
      901 gcgggagtgg cagtgggaat tcggcctcga tggccgcctg aagtgcctcg tcattggcct
      961 gccgatcctc gcggatcagg cgcagcggga cgcctatcag gtcaccaggc catggccgcc
     1021 gtggtcgtag gaaaaacgat ctagcgcgaa gggccaaagc ttgccaaatc taaacaaagt
     1081 tttagattta agggaataaa agtaccggtt cgcgcatcta tctccctccg gcccggtgcc
     1141 gcgttacgct ctttgacaat ttgcgatttc tgaagagctt ggagaaggtg ggagtgctgg
     1201 ccgcttcgtc gagaatgttg cgctcggagg catccaaaag cgcaacagcg caacatggct
     1261 ccgtcgcgct gtgccttgac gtaagtgctg aaatgctctg gtcttgcgag aatagttgcg
     1321 cgggacgttg cgcgaaaaaa aagttgcgcc gaaagcgcaa catcggaggg gcaatgttgc
     1381 gcgaggaggg ggaaacaaca ggttcccgat atcagatctg cctggtgaag agcgacttca
     1441 ggcgatccgc agcaggtcga ccggcaggca acttgtccgc gtggggcggg cttcgtcgcc
     1501 gtgcgagccg gggatggctc gccagagggc gtcgaggagg acgccgaggg agatgcggat
     1561 gggccggatg ccgggagcgt agtgctcgac ctcgtccagg cgctgcacgg tctcgggcag
     1621 cgtgacggcc aggttgtcga tcgttccccg gtagccgcga atccagttgg gctgcgaaac
     1681 accgggctgc gtcatggcga ggccggacgg acgcgagcgc cgcggttggg aagccgtgcc
     1741 cgggctgggc gtcgattgct tcgcgacttg gtcgccgggt cgcggcatgg ggccgcggcg
     1801 cgcctgccag atgagagaaa caatcagcag catgacggct gcggtggaca agacagtggc
     1861 caaggcccag acgatttttc gcgaggaaga agcgggcatg gcgcgagcgg acaaccgctc
     1921 ggccgacgca gcttgggcga caacgcggcg ggagaagccg gacgagaccg gcggcggcga
     1981 aaagcgccgc aggccggcga gcagcatctc ctggccaagg agcagttggc ggcagtcgtc
     2041 gcacgtttcg gcgtgggcgg caagctgggc atcggctcgc ggctggccgc ggtcgtcgag
     2101 gatgtggttc agtcgggttt cgaattcgtg gcagtgcatg tttgcgaaat caggtttcag
     2161 cgacgtttcc cagggcgaca tgttcgggga tcgcgccgcg cttgcgaagg cagtcgatca
     2221 gctcacggcg ggcgcggtgg acccaggttt tgatcgtccc cagcgggact tccatggcgg
     2281 cggcgatatc ggcgtagcac agctcctgtt cgtggaacaa gacgaatgcc tggcggtact
     2341 catctcgcag cgtggccaac gccagctgga cctcttcggc gaggtgctgg gccgggcggt
     2401 ggtcgggctc cttatcggga atga
//
LOCUS       DASUGB010000720         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2025555, whole genome shotgun sequence.
ACCESSION   DASUGB010000720 DASUGB010000000
VERSION     DASUGB010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025555"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFV87_13810"
     CDS             <1..570
                     /locus_tag="VFV87_13810"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5104889.1"
                     /translation="VANFSGQRDWQGKNLVQIAKENNKTPLEIVLTIQSRGGAAMINF
                     GMQEDEVRLFMREPFVATASDGSSMTLDSPSQPHPRAFGCFPRKIGYYSIEGHVVPLE
                     QAIRSASGLPADILGLKDRGYLKPGYHADVVVFDPATFRDTATFVKPLQWATGVKLLL
                     VNGQVAVEDEKPTGKLAGQAIRHPQGGKS"
     gene            599..1966
                     /locus_tag="VFV87_13815"
     CDS             599..1966
                     /locus_tag="VFV87_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5104890.1"
                     /translation="MPLSRRDCLTLASAGALAAGAWPAAMLQAETSPLGRSGKDLQIK
                     EVRITPIALPDPPLLAASGCHGPYFLRSVVEIDTADGITGIGETQGGQEVVGELQKAA
                     KTLVGKTALTYRKSAGSLLEANRRAYAGVELACLDAIGKAAGLRLCDLFGGPVRDDPE
                     FSSYLFFRYAADHPVLLADKRIVDRRGKGEQALDQWGEIRTPEAMAELAWLFKLKFGF
                     RVHKLKAGVLAPEVELEALRAISERCGGKDLVRIDPNGRWSLDTALKVAAGLKKLPLE
                     YYEDPVRGQEAMGQVRRDSGLKMSTNSCVVSFDDIPPAAVNKPVDVVLADHHYWGGIP
                     ACLALGKLAPAFGWSLSQHSNSHAGITMAAMIHVAATIPELTVASDTHYPWLIDTADI
                     IAGGKLPIVGGRMKIPAGLGLGVELDRDKLAQAHETYQKCGMRDRDDETTMRMVQPGW
                     ERTIL"
     gene            1988..2734
                     /locus_tag="VFV87_13820"
     CDS             1988..2734
                     /locus_tag="VFV87_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5104891.1"
                     /translation="MTKQAAKVALITGGGTGVGRSTTLQLAQRGYNVAVNYSRSQDEA
                     EATAAEAGEHGVRSIAVACDVSDDLQVRAMVEQCRREFGRLDVLVNNAGTTHFVPHTS
                     LEELTEDKWDRILAVNLKGPFFVSRAAIPLMQASGGGSIVNVASVAGVAGSGSSIAYA
                     ASKGGLITMTKSLAKAFAPQIRVNAVCPGVIISRWLEGHEDMVEAALKITPLKRASST
                     DDIADVIVFLACDAGMMTGQALVVDGGRTM"
     gene            complement(2748..>3516)
                     /locus_tag="VFV87_13825"
     CDS             complement(2748..>3516)
                     /locus_tag="VFV87_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5104892.1"
                     /translation="ALFHISLVLVLLLGLMLLPGDAAAGWRHGYGGYGYGGYRSCGYG
                     YGGYGYGAYGSCGYGYGGYGYGGYGYYGGGYWGGYAGYSPAVNYPYFYSGRTYSYGST
                     ETSPSDMRPGSGLAYGAEQNTRQPYRTASSSGGPATVVEVAAQDNYFQTNSLTVKPGT
                     TVRWTNRGTHPHTVTSNHDLFDSGEMQPGATYSATFQHPGNYYYYCRHHTGEAMRGMI
                     VVQPEDAPVASQQPAAAAAETARQPGTAGGPGPAQNY"
BASE COUNT          646 a         1139 c         1143 g          588 t
ORIGIN      
        1 gtggcgaatt tctccggcca gcgcgattgg caggggaaga atctcgtgca gatcgccaag
       61 gagaataaca aaacaccgct cgaaatcgtc ctcaccatcc aaagccgcgg cggcgcggcc
      121 atgatcaact tcggcatgca ggaagacgag gtgcggctgt tcatgcgcga gccgttcgtg
      181 gccaccgcca gcgacggcag ctccatgacc ctcgattccc ccagccagcc gcacccgcga
      241 gccttcggct gctttccgcg gaagatcggc tattactcga tcgagggcca tgtggtcccg
      301 ctagagcagg ccatccgcag cgccagcggc ttgccggccg atattctggg cctgaaggac
      361 cgcggctacc tgaagccggg ctatcacgcg gatgtcgtcg tcttcgatcc agccacgttt
      421 cgcgacaccg ccaccttcgt caagccgctg caatgggcga ccggcgtcaa gctgctgctc
      481 gtgaacggcc aggtcgccgt ggaagacgaa aagccgaccg gcaagctcgc cggccaggcc
      541 atccgccatc cgcaaggcgg aaagtcgtag tcgctcgatc cagcgcggag gtccgttcat
      601 gccactttcg cggcgcgatt gtttgacgct cgccagcgcc ggcgcgctgg ctgccggcgc
      661 gtggcccgcg gcgatgttac aggcggaaac gtcgccgctg ggccgctccg gcaaggacct
      721 gcagatcaag gaggtccgca tcacgccgat tgctttgccc gatccgccgc tgctcgccgc
      781 ttcgggctgt catgggccgt acttcctgcg gtccgtcgtc gaaatcgaca cggcggacgg
      841 catcacggga atcggcgaga cgcaaggcgg ccaggaagtc gtgggcgagc tgcaaaaggc
      901 ggccaagacg ctcgtcggca agaccgctct gacatatcgg aagagcgccg gttcgctcct
      961 ggaggccaat cgccgcgcgt acgctggcgt cgagctcgcc tgcctcgacg caatcggcaa
     1021 agcggcgggc ctgcggctgt gtgacctttt tggtggcccg gtccgagacg atcccgagtt
     1081 ctcctcctac ctgtttttcc gctacgccgc cgaccacccc gtgctgctgg ccgacaagcg
     1141 gatcgtcgat cgccgcggca agggcgagca ggctctcgac caatggggcg aaattcgcac
     1201 gccagaggcg atggccgaac tggcctggct gttcaagctg aaattcggct ttcgcgtcca
     1261 caagctcaaa gcgggcgtcc ttgctcccga agtcgaattg gaagcactgc gggcgatcag
     1321 cgagcgctgc ggcggcaaag atctcgtgcg gatcgatccg aatggccgct ggtcgctgga
     1381 cacagccctg aaagtcgccg ccgggctcaa aaagctgccc ctggagtatt acgaagaccc
     1441 cgtccgcggc caggaggcga tgggccaggt gcgccgcgat agcggcctga aaatgagcac
     1501 gaattcctgc gtcgtctcgt ttgacgacat cccgcccgca gcggtgaaca aaccggtcga
     1561 cgtcgtcctg gccgatcatc actactgggg cggcatcccg gcgtgcctgg cactcggcaa
     1621 gctcgccccc gccttcggct ggtcgctcag ccagcacagc aacagccacg ccggcatcac
     1681 gatggccgcg atgatccacg ttgccgcgac gattcccgag ctgacggtcg ccagcgacac
     1741 acactatccc tggctcattg atacagccga catcattgcc ggcggaaagc tgccaatcgt
     1801 cggcggacgg atgaagattc ccgccggact gggactgggc gttgagctcg accgcgataa
     1861 gctcgcccag gcccacgaga cctaccaaaa atgcggtatg cgtgatcgcg acgacgaaac
     1921 gacgatgcgc atggttcagc ccggctggga acgcaccatt ctgtaagtga aatacggagt
     1981 tcgcccaatg acaaagcagg cagccaaagt tgccctgatc accggcggcg gcacgggcgt
     2041 cggccgctcg accacgctgc agcttgccca gcgcggctac aacgtggccg tcaattactc
     2101 gcgctcgcag gacgaggccg aagcgaccgc ggcggaggcg ggcgagcacg gcgtgcggtc
     2161 catcgccgtt gcctgcgatg tttcggacga cttacaagtc cgggcgatgg ttgagcagtg
     2221 ccgccgtgag ttcggccggc tggatgtgct ggtcaataac gccgggacga cgcacttcgt
     2281 gccacatacc agcctggaag agctgaccga ggacaagtgg gaccgcatcc tggcggtcaa
     2341 cctcaagggg ccattcttcg tcagccgggc ggcgattccg ctgatgcagg ccagcggtgg
     2401 cggatcgatt gtgaatgtgg cgtcggtcgc gggcgtggcc ggctcgggga gttcgatcgc
     2461 ctacgcggcg tcgaagggcg gactgatcac catgaccaag tcgctggcca aggccttcgc
     2521 gccgcagatt cgcgtcaacg ccgtctgccc cggcgtgatc atctcccgct ggctggaggg
     2581 gcacgaagac atggtcgagg ccgcgctgaa gatcacgccg ctcaaacgtg cgtcatcgac
     2641 agacgacatc gccgacgtga tcgtgtttct cgcctgcgat gccggcatga tgaccggcca
     2701 ggcgttggtc gtcgatggcg gccggacgat gtagcccgtc cggcggctca gtaattttgc
     2761 gcggggcctg gcccgccggc ggtaccgggc tggcgggcgg tttcggctgc cgcggcggcc
     2821 ggctgctggc tggcaacggg cgcatcctct ggctgcacga cgatcatgcc ccgcatggct
     2881 tcgcccgtgt ggtggcggca gtagtagtaa tagttgcccg gatgttgaaa cgttgcgcta
     2941 taggttgcgc cgggctgcat ttcgccggag tcgaacaggt catggttgga agtcaccgtg
     3001 tgcgggtgcg tcccgcggtt cgtccaccgg acagtcgtcc ccggtttcac ggtgaggctg
     3061 ttcgtctgaa agtagttgtc ctgagcagcc acttcgacca ccgtcgccgg cccaccggaa
     3121 gaacttgcag tccggtacgg ctggcgggtg ttttgctctg cgccatacgc gagcccgctg
     3181 cctggccgca tatccgacgg cgacgtctcg gtcgagccat agctgtacgt ccggcctgaa
     3241 tagaagtacg gataattgac cgctggcgag tagccggcat agcctcccca atagccgccg
     3301 ccataatatc cgtagccacc gtagccatag ccgccatagc catagccgca ggagccatag
     3361 gccccatagc catagccgcc gtagccgtat ccgcaggagc gataaccgcc atagccatag
     3421 ccgccatagc catgccgcca gccggccgcg gcgtcgccgg ggagcagcat caagccgaga
     3481 agcagcacca ggaccaggga aatgtgaaag agagca
//
LOCUS       DASUGB010000721         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_358247, whole genome shotgun sequence.
ACCESSION   DASUGB010000721 DASUGB010000000
VERSION     DASUGB010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358247"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1788
                     /gene="pruA"
                     /locus_tag="VFV87_13830"
     CDS             196..1788
                     /gene="pruA"
                     /locus_tag="VFV87_13830"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX5104893.1"
                     /translation="MPITFTNQPPIDFAQADHRRAMLGALETVRDRLGVHQPLVIGGQ
                     EIESSERGQSFDPSHKSRLVGTFALATREHALQAVAAAKSALPGWAALPVERRAEYLE
                     KAADVLRDHLFEYAAWEVFECGKGWREATADIDEAIDFCEYYAAEAIALQSEQGVDVP
                     GEENRFDYLPRGVVGVIAPWNFPLAILTGMSVAALATGNTVVMKPSEQSAIVGAILMD
                     AFREAGLPAGVLNYLPGRGEIAGAALVEHPDVALIAFTGSRAVGLAINAQAAEVSKQG
                     VVNVKRVIAEMGGKNAIVVAADADLDEAVAGVVKSAFGYQGQKCSACSRAIVVGDVYE
                     PFLKRLIEATKSLKLGPAEEPATSVGPVIDEESYSRIREYIEIGKSESRLALAIDPGP
                     LADEGWFIGPHVFAEVPPTARIAQEEIFGPVLAVIRAADLTEALRIANGTDYALTGGI
                     FSRSPLALDRARREFLVGNLYLNRPITGALVGRQPFGGFKMSGIGSKAGGRDYLLQFV
                     LPRTITEHTVRRGFAPPADEGE"
     gene            1794..2534
                     /locus_tag="VFV87_13835"
     CDS             1794..2534
                     /locus_tag="VFV87_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5104894.1"
                     /translation="MKLLVLGTAGYHPSERRQTTCLMLPEAGIVLDAGTGFFRVRKYL
                     QTPTLDILLTHAHLDHVVGLTYLLTATWEKGIERVTIRGEASKLAAIRTHLLAEELFP
                     VPLPCQWQALEPGELSIGDIRVTHFPLPHLGGSVGYRLDWPGRSLAYVTDTTAAPDAA
                     YVESIRGADLLVHECNFRDGFEAWAAETGHSCTSAVAHVAQAAGVQRLVLMHFHPLDE
                     SDDPIGLEAARAIFPATDLGCDGMEIEI"
     gene            complement(2548..>4179)
                     /locus_tag="VFV87_13840"
     CDS             complement(2548..>4179)
                     /locus_tag="VFV87_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5104895.1"
                     /translation="DDGFPEGIVVTEAFRREVAEQGVRGVAFHDPRLWRPRHFPAETV
                     YLFSVPGKLDSAWNRPVEGDRGLLREAVRAGQAWRTILFAKRLLQTHSTDEEAEEALE
                     GLGPHQLTAKLLSSDNHARPQERIVPNAFLTPLDSATRLGVISACQLMERRRGQRLCQ
                     AGEPGDSMFIVLEGSVGVFRTESDAEPRYTATAGEILGELAFTLRRPRTAALLALDDA
                     SLLSIEPAALKAQAAANPVLAASVDRFLAARILEYTCNTRPYLVGPRRDGPLAELMKN
                     RSWDRLLPHSEMIVCEAREAEPVSLADPRFSGSGLYILVSGRLRSVTHADKVLDEADL
                     PLVFVDLPGRIACPDHRYRPEGGSATLLRIAPDAFLGRRPTIDAVAAEAARHIGHLYY
                     YDVFLSYTFDDHEAASRWREALEAAGFRVYMEVARSGHYFRDRIADGILDSKLLVALV
                     SANTMSRPLAQNWVRHEVAFRQTAFEMTTAKIVPIRLRGGKPELLADGYTIIESVGRE
                     SQAIDETVSAVRDTCEGRATLPYAYNRKMGLRIET"
BASE COUNT          714 a         1380 c         1374 g          711 t
ORIGIN      
        1 ctcctggaga acacgtcgaa cgattcgttc ctgcgggccg gtttcatgga cgacattccg
       61 gtcgaggagc tgctggaaaa tcccgaacag attgttctta gccgggagca aacttgctcc
      121 cggagtgcag gtgtggacga tctacccgca gttcgccggg accaggtttg gtcccggcta
      181 aatgagagtg ccaccatgcc aatcaccttc accaaccaac cccctatcga cttcgcccag
      241 gctgaccatc ggcgggccat gctgggtgcg ctcgagacgg tccgcgatcg gctcggcgtg
      301 catcagccgc tggtcatcgg cgggcaggaa atcgagtcct ccgagcgggg ccagtcgttc
      361 gatccgtcgc acaaatcgcg gctggtcggc acgtttgccc tggccacgcg cgagcacgcg
      421 ctgcaagccg tggccgcggc aaaatccgcc ctgccaggct gggccgcgct gcccgtggag
      481 cggcgggcgg agtatctgga gaaagcggcg gacgtgctgc gcgaccacct gttcgaatac
      541 gccgcgtggg aagtcttcga gtgcggcaaa ggctggcgcg aggcgacggc cgacatcgac
      601 gaggcgatcg acttctgcga gtactacgcc gccgaggcga tcgccctgca aagcgagcag
      661 ggcgtggatg tgccgggcga ggagaaccgc ttcgactatc tgccgcgggg cgttgtcggc
      721 gtcatcgcgc cgtggaattt tccgctggca atcctgaccg gcatgagcgt cgccgcgctg
      781 gcgaccggca acacggtggt catgaagccc tcggagcaat cggcgatcgt cggcgcgatt
      841 ctcatggacg ctttccgcga agccggccta cccgcgggcg tgctcaacta tctgccggga
      901 cgcggcgaaa ttgccggcgc ggcgctcgtc gagcatccgg acgtggcctt gatcgccttc
      961 accggctcgc gggcggtggg cctggcgatc aatgcgcagg cggcggaggt ttccaagcag
     1021 ggcgtggtaa acgtcaagcg cgtcattgcc gaaatgggcg gcaagaacgc gatcgtcgtc
     1081 gccgccgatg ccgacctgga cgaagcggtc gccggcgtcg tcaaaagcgc cttcggctat
     1141 caggggcaaa agtgctcggc ctgcagccgg gcgattgtcg tgggcgacgt ctatgagccg
     1201 ttcctcaagc gcctgatcga ggcgacgaag agcctgaagc tcggcccggc cgaggagccg
     1261 gcgacgagcg tcggcccggt gatcgacgaa gagtcgtaca gccgcatccg cgagtacatt
     1321 gagatcggca agagcgaatc gcgattggcc ctggcgatcg atcccggccc gctggccgac
     1381 gaaggttggt tcatcggccc gcacgtcttt gccgaagtgc ctccgacggc tcgcattgcc
     1441 caggaagaga ttttcggccc cgtgctcgcc gtcatccggg ccgccgacct gaccgaagcc
     1501 ctccgcatcg ccaacggcac cgattacgct ctgaccggcg gcatcttcag ccgcagcccg
     1561 ttggcgctgg atcgcgcccg ccgcgagttc ctcgtcggca atctctatct caaccggccg
     1621 atcaccgggg ctcttgtcgg ccgccagccc ttcggcgggt tcaaaatgtc gggcatcggc
     1681 agcaaagcgg gcggccgcga ctacctcttg cagttcgtcc tcccccgcac gatcaccgag
     1741 cataccgtcc gccggggttt cgccccgccc gccgatgagg gagagtagaa cgcatgaaac
     1801 tcctcgtcct cggcacggcc ggctatcatc ccagtgagcg gcggcagacg acttgcctca
     1861 tgctgcccga ggcggggatc gtgctcgatg cggggacggg tttttttcgc gtgcgcaagt
     1921 acttgcagac gcccacgctc gacatcctgc tgacgcacgc ccatctggat cacgtcgtcg
     1981 ggctgacgta cctcctgacg gcgacctggg aaaagggcat cgagcgggtg accatccgcg
     2041 gggaggcatc caaactggcg gcgattcgca ctcacctgct cgccgaggag ctgtttcccg
     2101 tgcctttgcc ttgccagtgg caggcgctgg agcccggcga gctgtcgatc ggcgacatcc
     2161 gcgtcaccca cttcccattg ccgcacctgg gcggatcggt tggttatcgg ctggattggc
     2221 cgggccgctc gctggcgtat gtcaccgata cgacagccgc accggacgca gcctacgtcg
     2281 aatccatccg cggggccgat ctgctcgtac acgagtgcaa tttccgcgac ggcttcgaag
     2341 cctgggccgc tgaaaccggc catagctgca cctccgcggt cgcccacgtg gcacaggcgg
     2401 caggggtcca gcgactggtg ctgatgcact tccatccgct cgacgaaagc gacgacccca
     2461 ttggcctgga agccgcccgg gccatcttcc cagcgaccga tcttggctgc gacggaatgg
     2521 aaatcgagat ctgaccgagt tgcggactca agtctcaatc cgcaggccca ttttgcgatt
     2581 gtatgcgtac ggaagcgtcg cgcggccttc gcacgtatcc cgtacggcgc tcacggtctc
     2641 gtcgatcgct tgcgactcgc gcccgaccga ttcgatgatc gtgtaaccgt cagccagcag
     2701 ttccggtttg cctcctcgca gccgaatcgg tacgattttc gcagtcgtca tctcgaaagc
     2761 tgtctgccgg aaggcgactt cgtgccggac ccagttctgc gcgagcgggc gcgacatggt
     2821 gttggccgag acgagagcca ccagcagctt ggaatcgagg atgccatcgg caatccggtc
     2881 gcggaaatag tgcccgctgc gggcgacctc catatagacg cgaaatccgg ctgcttccag
     2941 cgcttcgcgc cagcggctgg cggcctcgtg gtcgtcgaac gtgtaggata aaaagacgtc
     3001 gtagtagtac aggtggccga tgtgccgcgc ggcttcggcg gcgacggcgt cgatggttgg
     3061 ccgccggccc aggaaggcat cgggagcgat ccgcaggagc gtggcgctgc ccccctcagg
     3121 acggtaccgg tgatcggggc aggcgatccg gcccggcaga tcgacaaaga cgagcggcag
     3181 atccgcttcg tccagcacct tatcggcatg cgtgacactc cgcaaccggc cgctcacgag
     3241 gatatacagc ccgctgccgg agaaccgcgg atcggctagc gaaaccggtt cggcttcgcg
     3301 cgcctcgcac acgatcatct ccgagtgcgg cagcaggcgg tcccaactgc ggttcttcat
     3361 gagttcggcc agcgggccgt cgcgccgggg gccgacaaga tagggccgcg tgttgcaggt
     3421 gtattccagg atgcgagcgg cgagaaaccg atcgacgctc gcggccagga cgggatttgc
     3481 cgcggcctgc gccttgagcg ccgccggctc gatcgacagc agcgacgcat cgtccaaggc
     3541 cagaagcgcc gccgttcgcg gtcggcgaag cgtgaaggcc aattcgccca ggatctcgcc
     3601 cgccgtggcc gtatagcgcg gctccgcgtc gctttcggtt cgaaaaacgc cgacgctccc
     3661 ttcgaggacg atgaacattg aatcgcccgg ctcgcccgcc tggcacagcc gctggccgcg
     3721 ccgccgctcc ataagctggc acgcgctgat gacgcccagg cgcgtggcgc tatccagcgg
     3781 cgtgaggaac gcattgggca cgatccgctc ttgcggcctg gcgtggttgt cgctcgagag
     3841 aagcttcgcc gtcaactgat gtgggccaag tccctccagg gcctcttcag cttcttcgtc
     3901 cgtcgaatgg gtttgcagca accgcttggc gaagagaatc gtccgccacg cctgtccagc
     3961 gcggaccgct tcgcgcagca ggccccggtc gccttcgacg ggccgattcc aggccgagtc
     4021 gagcttgccc ggcacggaaa acaggtacac ggtttcggcc ggaaagtggc gcggccgcca
     4081 gaggcgcgga tcatgaaacg caactccgcg cacgccttgc tccgcgactt cccggcggaa
     4141 ggcctcggtc acgacgattc cttcgggaaa accgtcgtc
//
LOCUS       DASUGB010000722         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_775058, whole genome shotgun sequence.
ACCESSION   DASUGB010000722 DASUGB010000000
VERSION     DASUGB010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775058"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFV87_13845"
     CDS             <1..418
                     /locus_tag="VFV87_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104896.1"
                     /translation="LERPQPDRDELDVAAHDVQAILLNGDSQALQRACTAEGWKYVNQ
                     CYDFHLGALGFKGTFADFMRDCGERMRASDPSDVVKESFNDGTAVIYFDYSFSEGVTI
                     AFCKRKGQWRIAGFSPGRIAPESFFPAGSKYPAALK"
     gene            complement(641..>2033)
                     /locus_tag="VFV87_13850"
     CDS             complement(641..>2033)
                     /locus_tag="VFV87_13850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5104897.1"
                     /translation="PITIDAMSLGLRWIALTMGLVFTLAVSRQADADLASELLGSLML
                     VTAGVMLAASANELVLLFLGLELISIPTYVLLFLGRRDRASSEATMKYFFLSIFSSAL
                     LLYGLSMVYGLAGTTIIAGNEDTRGIRELIAGLADNGSPLAALAPLALVLLVAGLGFK
                     LAIAPLQFYAPDVYQGTTNVNAGLLAVAPKIAGVAGLVRLALVAMPAAGEFGWQLVLV
                     LSVLTMTIGNICALWQQNVRRLMAYSSIAHGGYLLIGLAAAAAATTPAGASFDGGIAS
                     MLIYVAVYAAATMGTFAALAWLGSTSREVNDVDELSGLGRSHPVAAGAIALFMFSLAG
                     IPPLAGFWGKLTLFGSAVQVAAAGAPGGLSLWFTVLAIVGVLNAAVAAAYYLRIVAVM
                     YFQPSSTTPQAAGGRGALAATLLCAGLVVLLGVLPGAALDSATEAETTLRHRAPLATA
                     KPAIRLPTTAQFR"
BASE COUNT          409 a          622 c          669 g          333 t
ORIGIN      
        1 gctagaacgg ccccagccgg atagggatga actcgacgta gccgctcacg atgtccaggc
       61 cattttgctg aatggtgaca gtcaggcatt acagcgggct tgcacagcgg agggctggaa
      121 gtacgtcaac caatgctacg actttcatct gggtgctctc ggttttaagg gtacgtttgc
      181 cgactttatg cgtgactgtg gggaaaggat gcgggcaagc gacccgtcgg atgtcgtcaa
      241 agaaagtttt aatgacggca ccgcggttat ttacttcgac tactcttttt ctgagggagt
      301 caccattgca ttctgcaaga ggaaaggaca atggcggatt gcggggtttt ctcctggtcg
      361 aattgcgccc gaatcctttt tcccagcggg atcgaaatat cctgcagcgc tcaagtgatg
      421 gcatggttcc tccgccgggt gccacataca gtaactatgt aagtggcgaa tcgcatttct
      481 atacttgggc gaaatcgatt cgaaccctgt accgcgaatc caaccgttga tccgcctgtc
      541 ggctcactct gttgttcgcc ttacggcaat cgtcaatggg aagccagttg tcgctaaatc
      601 gcaatcgctt gcgacagcat ctgatttttg gggtaaagcg ctaccggaat tgtgctgtcg
      661 tcggcaggcg aatggccggc ttggcggtgg ccagcggcgc tcggtgacgg agcgtggttt
      721 cggcttcggt ggccgaatcg agggccgcgc cgggcaggac gcccaggagc accaccaggc
      781 cggcgcagag cagggtcgcg gccagagcgc cgcggccgcc ggctgcctga ggcgtggtgc
      841 tggacggttg aaagtacatc accgccacga tccgcaggta ataggccgcg gccaccgcgg
      901 cattgagcac gccgacgatc gccagcaccg tgaaccagag cgacaggccg ccgggtgcgc
      961 ctgcggctgc cacctggacg gcgctgccaa acagcgtgag cttgccccaa aacccagcca
     1021 gcggcggaat gccggccagc gagaacatga acagggcaat cgccccggcc gccacggggt
     1081 gcgagcggcc caggccggac agttcgtcca cgtcgttcac ctcgcggctg gtcgagccca
     1141 gccaggcgag cgccgcgaac gtgcccatcg tggcggcggc atagacggcg acatagatca
     1201 gcatggacgc gatcccgccg tcgaagctgg ccccggccgg cgtggtggcg gcggccgcgg
     1261 cggccaggcc gattagcagg tagccgccgt gggcgatcga cgaataggcc atcagccggc
     1321 ggacgttctg ctgccacaag gcgcagatgt tgccgatggt catggtcaaa accgacaaca
     1381 ccaggaccag ctgccagccg aactcgcccg ccgcaggcat ggccacgagc gccaggcgga
     1441 caagaccggc caccccggca atttttggcg ccacggccag gagccccgcg ttgacgttgg
     1501 tcgtgccctg gtacacgtcg ggtgcataga actgcaaggg cgcgatcgcc agcttgaagc
     1561 ccagcccggc aacgaggagc acgagcgcca gcggcgcgag tgctgccagg ggactgccgt
     1621 tgtcggccag gccggcgatc agctcgcgaa tgccgcgggt gtcttcgttg ccggcgatga
     1681 tcgtcgtgcc ggccaggcca tagaccatcg acaggccgta cagcagcaag gcggacgaaa
     1741 agatgctcag gaaaaaatac ttcatcgtcg cttccgacga cgcccggtcg cggcggccga
     1801 ggaacaagag cacataggtc ggaatggaaa tcagctccaa gcccaaaaac agcagcacca
     1861 gctcgttcgc cgacgcggcg agcatcacgc ccgccgtcac cagcatcagg ctgccgagca
     1921 gctcgctggc caggtcggcg tcggcctgcc gcgagacggc gagcgtgaag acgaggccca
     1981 tggtcaaagc gatccaccgc aggccgaggc tcatggcgtc gatggtgatc ggc
//
LOCUS       DASUGB010000723         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_358262, whole genome shotgun sequence.
ACCESSION   DASUGB010000723 DASUGB010000000
VERSION     DASUGB010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358262"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1565)
                     /locus_tag="VFV87_13855"
     CDS             complement(123..1565)
                     /locus_tag="VFV87_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5104898.1"
                     /translation="MFARTYSVGILLGLAFLATSVTLVAAERPNIVVILCDDLGYGDL
                     GCYGHPAIKTPNLDALAAQGMRFTDCYSAAPVCSSSRAGLMTGRTPSRMGIYDWIPAN
                     HPMHLAKDETTVATLLKQAGYSTCHVGKWHLNGKFNSPEQPQPGDHGFDHWMSTQNNA
                     APSHANPRNFVRNGTPLGPQEGYSCQLVAQEAIRWLCELKDKSKPFFLFVCFHEPHEP
                     VASPPDLVATYPDAKNPDQAEYFANVTNMDAAVGKLIGKLDELKLTEHTLVFFTSDNG
                     PETLLRYPNGKRSYGSPGPLRGMKLHLYDGGFRVPGIVRYPVRVKAGQTSSEPVCSLD
                     FLPTFCELAGVKLPENRTFDGVSFLPVFDGQPVPRNVPLYWHYYRSIGEPKAAMRVGD
                     YMILGKWDGPDFEPGGSVHYGDCDVIKSAKLVGFELYNLKADLGEQTDLAAAEPEKLK
                     ELSALLVAKYNEVQAAGPVWDVPKPKGKEK"
     gene            complement(1702..3201)
                     /locus_tag="VFV87_13860"
     CDS             complement(1702..3201)
                     /locus_tag="VFV87_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5104899.1"
                     /translation="MPRTNHVLLIANPLVPMMYSARFFLCAAALLVGAVSAALAAEPA
                     SHPNIIFILADDLGYGDLGCYGQQRIKTPHIDGLAREGMRFTDFYAGCTVCAPSRCVL
                     MTGYHMGHCHVRGNAGQQNRDAQILRDQDVTIAELLKRAGYATALCGKWGLGEENSIG
                     EPTKQGFDYFFGYLNQHHAHNYYPAYLFQNEKRYPLKNVVPGGGDFGSGVATRKVEYS
                     ADLIAAEALKWVEEHKDRPFFLYFAATIPHANNEAGKEGMEVPDLGQYADKDWPPQQK
                     AHAAMISRLDADVGRLMALLKKLGLDDNTLVFFSSDNGPHREGGNDPDFNDSNGPLKG
                     IKRDLTEGGIRVPFIARWPGHIKAGTTSDFVGSFQDMLPTLAELAGASADVPNDIDGL
                     SIVPTLLGQPEKQQQHDYLYWAFYERGGAQAARAGRWKAVQQPLHTPVRLYDLSADIG
                     EEHDLAAKQPETVAKLAGFMREAYRPSENWRFPDPPPAGKAGTAKKATK"
     gene            3327..>3591
                     /locus_tag="VFV87_13865"
     CDS             3327..>3591
                     /locus_tag="VFV87_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5104900.1"
                     /translation="MLRALRLPCLVILASLIPWPAFAQQPQAKKKAPNPAYAQIEDVA
                     GLPRVLLIGDSISIGYTVAVREELKGKANVHRPATNCGPTTRGV"
BASE COUNT          637 a         1109 c         1140 g          705 t
ORIGIN      
        1 aaacggccgc gtacgatgga tcatggacgc gttgtaaatt tcaggacacc ctcaagcgga
       61 gcttgggaac gaggtccaga agagtgccag cgatactccg agcctcttga cggcatgcac
      121 gttcatttct ctttcccctt cggcttgggc acatcccaca cggggccggc ggcttgcacc
      181 tcgttgtact tggcgacgag cagggcggaa agctccttga gcttttccgg ctcggcggct
      241 gccagatcgg tctgctcccc gaggtcggcc ttcaggttgt atagctcgaa cccaaccagc
      301 ttggccgatt tgatgacgtc gcaatctcca taatgtacgc tgccgccggg ctcgaaatcg
      361 ggaccatccc acttgccgag gatcatgtag tcgccgacgc gcatcgccgc tttcggctcg
      421 ccgatcgagc ggtagtagtg ccaatacagc ggcacgttcc gcggcacggg ctggccgtcg
      481 aaaacgggca ggaaactgac gccgtcaaac gtgcggttct cgggcagctt cacgccggcc
      541 agttcgcaga acgtggggag aaagtccagg ctgcagaccg gctcgctcga ggtttgtccc
      601 gccttcacgc ggaccggata acgaacgatc cccggcacgc gaaagccgcc gtcataaagg
      661 tgcagcttca ttccccgcag cggccccggc gagccgtacg accgcttgcc attcggatag
      721 cggagcagcg tttccgggcc attatcggag gtgaagaaga ccagcgtgtg ctccgtcagc
      781 ttcagttcat cgagcttgcc aatcagcttg ccgaccgccg catccatatt cgttacgttc
      841 gcgaaatact ccgcctggtc gggattcttg gcgtccggat aggttgccac caggtccggc
      901 ggcgaagcca ccggctcgtg cggctcgtgg aagcagacga acaggaagaa cggcttgctc
      961 ttgtctttca gctcgcagag ccagcggatc gcctcctggg cgacgagctg gcacgaatag
     1021 ccctcctgcg ggccgagcgg cgtgccgttg cggacgaaat tccgcgggtt ggcgtgcgac
     1081 ggggccgcat tgttctgcgt gctcatccag tggtcgaagc cgtggtcgcc gggctgcggc
     1141 tgctcgggcg agttgaattt gccgttgagg tgccacttgc cgacgtgaca agtcgaataa
     1201 cccgcctgct tgaggagcgt agcgacggtc gtctcgtcct tggccaggtg catcgggtgg
     1261 ttggcgggaa tccagtcata aatgcccatg cggctgggcg tgcggccggt catcaagccg
     1321 gcccgcgagc tgctgcagac cggcgcggcc gagtagcaat cggtaaaccg catgccctgg
     1381 gctgcaagcg cgtcgagatt aggtgtcttg atcgccggat ggccgtagca gcccaaatcg
     1441 ccgtagccga ggtcgtcgca cagaatgacg acgatgttcg gccgctcggc agcgacgagc
     1501 gtcacgcttg tggcgagaaa cgccaggccc aggagaatgc caacagaata ggtgcgagcg
     1561 aacatggcgg agacccttcg agaagcaatg tccgagatga gactcgtggc ggcgcgcgtc
     1621 cacctccgcg caaactgccg tccctgttag cgtaatcctg cgcagccggg cttgccaatg
     1681 caaattgctt tgcggcaggc cctatttggt cgccttcttc gcggtccccg ctttgcccgc
     1741 gggcggagga tcggggaaac gccagttctc gctcggccgg taggcttcac gcatgaagcc
     1801 cgccagtttg gcaacggttt ccggttgctt tgcggccagg tcgtgctctt cgccgatgtc
     1861 cgccgacagg tcgtacagcc gcaccggcgt gtgcagcggc tgctggacgg ccttccagcg
     1921 gccggcgcgg gcggcttgtg cgccgccccg ttcgtagaac gcccagtaga gatagtcgtg
     1981 ctgctgctgt ttttcgggct ggccgaggag tgtcgggaca atcgacaggc cgtcgatatc
     2041 attcggcacg tcggctgaag cgccggccag ttccgccagc gtcggcagca tgtcctgaaa
     2101 gctgccgacg aaatcgctgg ttgtgccagc cttgatgtgg cccggccagc gggcaatgaa
     2161 cggcacgcga atgccccctt cggtcagatc gcgcttgatc cctttcagcg ggccgttgct
     2221 gtcgttgaaa tccggatcgt tgcccccttc gcggtgcgga ccgttgtcgg acgaaaagaa
     2281 gacgagcgtg ttgtcatcga ggcccagctt cttcagcagc gccatcaagc ggccgacgtc
     2341 ggcatcgagg cgcgagatca tggccgcgtg ggctttctgc tgaggcggcc agtctttgtc
     2401 ggcatattgc ccgaggtcgg gcacttccat gccctctttg ccggcttcgt tgttggcgtg
     2461 cggaatcgtc gcggcgaaat agaggaagaa cggccggtcc ttatgctctt cgacccactt
     2521 caaagcttcg gcggcgatca ggtcggcact gtattcgacc ttcctggtcg ccacgccgct
     2581 gccgaagtcg ccgccgccag gcacgacatt cttgagcgga taacgcttct cattctggaa
     2641 caagtaggcc ggatagtaat tgtgggcgtg gtgctgattc agatagccga aaaagtagtc
     2701 gaagccctgc ttcgtcggtt cgccgatcga gttctcttcg cccagtcccc atttgccgca
     2761 gagggccgtt gcatagccgg cgcgcttcag caattcggcg attgtcacat cctgatcgcg
     2821 caagatctga gcgtcgcggt tctgctgtcc cgcattgccg cggacgtggc aatgccccat
     2881 gtgatagccg gtcatcagca cgcaccgcga cggcgcacag accgtgcagc ccgcatagaa
     2941 atcggtgaag cgcatccctt cgcgagccag cccgtcgata tgcggcgtct tgatccgctg
     3001 ctggccgtag cagcccaggt cgccatagcc caagtcatcg gccaggatga agatgatatt
     3061 ggggtggctg gccggctcgg cagccaaagc cgctgagacc gcgccaacta aaagcgcggc
     3121 ggcacaaagg aagaagcgag cgctgtacat catgggaacg agcgggttgg cgatcagcag
     3181 gacgtgattc gttctcggca ctcagtactt tagcattgcc ttctcgcgac tcgcaattcg
     3241 ccggccgact ggcttcgcgg ggcgcggccg cctatcatac ggccaagcac atccaccttt
     3301 tggccgctcc tgggggggaa ttccacatgc ttcgcgctct tcggctcccg tgtctcgtca
     3361 ttctggcgtc gttgatcccc tggccggcat tcgcccagca gcctcaggcg aagaagaaag
     3421 cccccaaccc ggcttatgcc cagatcgaag atgtagctgg gctgccgcgc gtgctcttga
     3481 tcggcgattc catttcgatc ggttatacgg tggccgtccg cgaagaactc aaggggaagg
     3541 ctaacgtgca ccggccggcg accaattgcg gtccgacgac gcgcggcgtc g
//
LOCUS       DASUGB010000724         6068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1885523, whole genome shotgun sequence.
ACCESSION   DASUGB010000724 DASUGB010000000
VERSION     DASUGB010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885523"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFV87_13870"
     CDS             <1..407
                     /locus_tag="VFV87_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthetase"
                     /protein_id="HEX5104901.1"
                     /translation="GPDGIGPLPELSVINDQAPTAELRPPSAKQTDEDDLMPYELLDA
                     IERAAIRDKQSPSEILLLMEVEFPQYSRGQLVTWIERFFRLWSRNQWKRERYAPSFHL
                     DDENLDPKTWCRFPILSGGYERELAELRKLAT"
     gene            complement(429..2303)
                     /locus_tag="VFV87_13875"
     CDS             complement(429..2303)
                     /locus_tag="VFV87_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104902.1"
                     /translation="MTRNQSFIRKVVYIALIALLLLPLSALSQPATVGPKDQPELSSP
                     GGKLSQLRVRYNLAQAELGEIDPASESMKLASLGLRGVAANILWSWANYYKKVEDWDK
                     VELTVNQIIRLQPNFLRVWDFQAHNLSYNISTEFDDYRMRYAWVKKGIDFLIEGTYYN
                     REEPGLLSQVGWYVGQKIGRSDEQKEFRVMFKDDEDFHHEFRDNGVEVDQLAALGPDA
                     KPDNWLVSRLWYNKATDSVTSRGKVIRGMSEVLFYGRPAMSQINAADAMQKDGFFFEV
                     AKRAWERAAEEWRTYGDRELPSVKGYTVRMNDREPLEAHIVELRRQIEELCPGLHAEV
                     RKVRLPQLNAEQRAAVEKPAEERTVEERFIADYAELALQASTDEIMERAPRDVRPQVR
                     KLVDQMREDEEIIHEIRANRNVVNFEYWRTRVEAEKTDVARQARKDVYDADQLKDKGE
                     EFDRARQLYERAWDGWAIIFKQYPELMNNAESQDLIKSVSRYRDLLGQLDQPFPVDFK
                     LNDLLDMHFEGQQLREQIRLIQGTGKPAKEESKPEETKPEESKPEESKPEESKTEETK
                     PEVTKPGESPPERAKPDEENKPAENKPQGNAKPVSPKAGAAAPSAGNETPSKDDKPSE
                     "
     gene            complement(2300..4381)
                     /locus_tag="VFV87_13880"
     CDS             complement(2300..4381)
                     /locus_tag="VFV87_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104903.1"
                     /translation="MALEDGIPNFWTWLLGDPVKASLFRPDSWGFLPGTIAICFILLL
                     LVPFFCFVVASFKYGPSEAFYYVARAMFSAVTEDLPRFSPRRTFAVARLAVQEAIRNR
                     VLYGFGVFIVLLLFAGLFLDVSNSNPARVYLSFVLHTTNYLVLLMALFLSTFSIPNDI
                     KNRTIYTVVTKPVRASEIVLGRILGFAAVGTAMLLAMGLIGYGFVWRGLAHSHRVDLE
                     SIVEEKDDNGKLVRITGQTTLDSHHRHTFELDATGKGKTDVVAHHWHAIQGRNLPHFF
                     ELVDLDRDVKLSREELQPPPEPDPQESENVRLIAAKRRELLAQIDLDFASADKDKDGF
                     ITKEEFPAPSYRVGPPEGQLVAKAPVYGALQIFDRNGNPAEKGINVGNEWEYRGYIEG
                     GTPGVQSRAAAVWTFSDITPEKYPEGLPLEMTIRVFRSYKGDIKRGVLGEIVVCNPNR
                     SAKVKRSGPILFESKEFVSDYKFINRTLRSEIGGSGGAGTIDLFDDLVDNGQVQIEIR
                     CVDAAQYFGVAAPDLYLRPADAAFEWNFVKAYLSIWLQMLLVTGFGVTFSTFLTGSVA
                     MMATLSAIVLGFFGQFVRDVASGAMMGGGPIESLIRIITQQNVMNELEVGGIVARVLK
                     SIDWVLMGLMTAGTYILPDYTQFDTATFVADGYNIFGDLVAQQIVMAAVYFVLVTIVG
                     YFFLKTREIAA"
     gene            complement(4465..5391)
                     /locus_tag="VFV87_13885"
     CDS             complement(4465..5391)
                     /locus_tag="VFV87_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104904.1"
                     /translation="MTTQLDAPIKAEAVGPSSEVIIETRNLTKVYRDFWGRQKVRALN
                     ALDLQVRRGEIFGLLGPNGSGKSTTIKLLLGLLFPTQGQALVFGKEATEVSKNERIGY
                     LPEESYLYKFLNAEETLDFYGRLFDMPASVRKQRTNELIEMVGLNWARRRQLREYSKG
                     MTRRIGLAQALINEPELILLDEPTSGLDPIGTREMKDLILKLREQGKTILMCSHLLPD
                     VQDVCDRIAILHQGELKELGRVDELLMVQDVTQFRAQGLSKEAEEEIRAVIARHGGQT
                     IAVEHPTMSLEELFLNIVRDSEARPGRRAGDK"
BASE COUNT         1159 a         1848 c         1793 g         1268 t
ORIGIN      
        1 agggcccgga cggcatcggc cccttgccgg agctgtcggt catcaacgac caggccccga
       61 ccgctgagct gcgcccgcct tcggccaaac aaaccgacga agatgacctg atgccttacg
      121 aattgctgga cgccatcgag cgggctgcca tccgcgacaa gcagtcgccg agcgaaatcc
      181 tgctcctcat ggaggtcgaa ttcccgcagt attctcgcgg gcagctcgtc acctggatcg
      241 agcggttctt ccgcctctgg agccgcaacc agtggaagcg ggagcgctac gcccccagct
      301 tccacctcga tgacgagaac ctcgacccca aaacctggtg ccgcttcccc atcttgagcg
      361 gcggctacga gcgggagctt gccgagctgc ggaagctggc aacctgaccg ccgcatcagg
      421 ggccgtggct actctgaagg tttgtcgtct ttcgaaggcg tttcgttccc ggctgatggt
      481 gcggctgcac ccgccttcgg cgacacgggc ttggcgtttc cttgaggctt gttctctgca
      541 ggcttgttct cctcgtccgg tttcgcccgc tctggcggcg actctcctgg cttcgtaact
      601 tccggcttgg tctcctcggt tttgctctcc tcgggtttgc tctcctcggg tttgctctcc
      661 tcgggtttgg tctcctcggg tttgctctcc tccttggcgg gctttccagt gccctggatc
      721 aagcggatct gctcgcggag ttgctgccct tcaaagtgca tatcgagcag atcgttgagc
      781 ttgaaatcga ccgggaacgg ttgatcaagc tggccgagca gatcgcgata gcgagaaacg
      841 gacttgatca aatcttggga ttcggcgttg ttcatcaatt cgggatactg cttgaagatg
      901 atcgcccaac cgtcccaggc ccgctcgtaa agctggcgtg cgcggtcgaa ttcctcaccc
      961 ttgtctttga gttggtcggc gtcgtaaacg tccttgcggg cctggcgggc cacgtcggtc
     1021 ttctccgctt cgactcgagt cctccagtat tcgaaattca cgacgtttcg attggcgcgg
     1081 atttcgtgga tgatctcctc atcttcgcgc atctgatcga caagtttgcg gacctgcggg
     1141 cggacgtcgc gtggagcgcg ctccatgatt tcgtcggtgc tggcctggag tgccagctcc
     1201 gcataatccg cgataaatcg ctcctcaacg gtccgctcct cggccggctt ctcgacggcg
     1261 gcacgctgct ccgcattgag ctggggcagg cgcactttgc gcacttcggc gtgcaaaccc
     1321 gggcagagct cttcgatctg tcggcgaagt tctacaatgt gcgcctccag cggctctcga
     1381 tcattcatcc gaacggtata tcccttgacc gagggaagct cgcggtcgcc gtaggtccgc
     1441 cattcttcag cggccctttc ccaagcacgc ttggcgacct cgaaaaagaa cccgtctttc
     1501 tgcatggcgt cggcggcatt gatctgcgac atcgccgggc ggccgtagaa cagcacttct
     1561 gacatcccgc ggattacctt gccacgggat gtcaccgaat ccgtcgcctt gttgtaccac
     1621 aggcgcgaaa ccagccagtt gtccggcttt gcatccggtc ccagggcggc gagctgatcg
     1681 acctccacgc cgttatcccg gaattcatga tggaagtcct cgtcatcctt gaacatcacg
     1741 cgaaattcct tctgctcgtc ggaccgaccg atcttttgcc cgacgtacca ccccacctgg
     1801 ctgagcaggc cgggctcctc gcggttgtaa taggtgcctt cgatgagaaa gtcgatcccc
     1861 tttttcaccc acgcgtaccg catccggtag tcgtcgaatt cggtcgagat gttgtacgac
     1921 agattgtggg cctgaaagtc ccagaccctg agaaaattcg gttggaggcg gatgatctgg
     1981 ttgacggtca gctcgacttt gtcccagtcc tcgacctttt tgtagtaatt ggcccagctc
     2041 caaaggatat tcgcggccac gccgcgcagg cccagggagg ccagcttcat cgattcgctg
     2101 gcggggtcga tttctcccag ctccgcctgg gccaggttgt acctcacccg caactgcgac
     2161 agcttgccgc cgggggaact gagctcgggc tggtccttgg ggccgaccgt ggccggctgg
     2221 ctgagggccg agagcggcag gagcaacagc gcaatcagtg cgatgtaaac caccttgcgg
     2281 atgaacgatt ggttgcgtgt catgcggcga tctcccgcgt cttcaggaag aaatagccga
     2341 cgatcgtgac caggacgaaa tagaccgccg ccatcacgat ctgctgggcc accaggtcgc
     2401 cgaagatgtt atagccgtcg gccacgaagg tcgccgtgtc gaactgagtg taatcgggca
     2461 aaatgtaggt tcccgcggtc atcaagccca tcaaaaccca gtcgatggac ttcagcacgc
     2521 gggccacgat tccgccgact tccagctcgt tcatcacgtt ttgctgcgtg atgatgcgga
     2581 tcagggattc gatcggcccg ccgcccatca tggctccgct ggccacgtcg cggacgaact
     2641 ggccgaagaa acccaataca atggctgaca gtgtggccat catcgccacc gagccggtca
     2701 ggaacgtgct gaaggtgacg ccgaagccgg tcaccagcaa catctgcagc cagatactga
     2761 gatacgcctt tacgaaattc cattcgaacg cagcgtcagc cgggcggagg taaagatcgg
     2821 gcgcggcgac tccgaaatat tgggccgcat cgacgcagcg gatctcgatc tgcacctggc
     2881 cgttatcgac caggtcgtcg aacagatcga tcgttccggc gccgccgctg ccgccgatct
     2941 cggacctgag cgtgcggttg atgaacttgt agtcggagac gaattccttc gattcgaaca
     3001 ggatcgggcc gctgcgcttc accttggcgg accgattggg attgcagacg acgatctcgc
     3061 ccaggacgcc gcgcttgata tcacccttgt acgagcggaa gacgcgaatc gtcatctcca
     3121 gcggaagccc ctcgggatat ttctccgggg taatatcgct gaacgtccag acggccgcgg
     3181 cccggctttg gacgcccggc gtaccacctt cgatgtagcc gcggtactcc cattcgttgc
     3241 cgacgttgat tcccttttct gcggggtttc cgttccggtc gaaaatctgc agcgcgccat
     3301 acacgggggc tttcgcgacg agttggccct ccggaggtcc aacgcggtaa cttggagccg
     3361 ggaactcttc cttggtgata aagccgtctt tgtccttgtc ggcggacgca aaatcaaggt
     3421 cgatttgtgc caggagctct cggcgttttg ccgcgatcag cctcacgttt tccgattcct
     3481 gtggatctgg ctctggcggt ggctgcagct cttccctgct caacttgacg tcgcgatcca
     3541 gatcaaccaa ctcgaaaaaa tgcggcaagt tgcgcccctg gattgcgtgc cagtgatgcg
     3601 cgacaacgtc agtcttgccc ttgccagtag cgtccagctc gaacgtatgg cggtggtgag
     3661 aatcgagcgt cgtctggccg gtaatccgga cgagctttcc gttgtcgtct ttctcctcga
     3721 cgatcgactc cagatcgacc cgatggctgt gcgcgaggcc gcgccacacg aacccgtagc
     3781 cgatcaggcc catcgccagc agcatcgccg tgccgaccgc ggcaaagccc aggatgcgtc
     3841 cgaggacgat ttcgctggcg cgaaccggct tggtgaccac ggtgtaaatc gtgcgattct
     3901 tgatgtcgtt gggaatgctg aacgtgctga ggaacagcgc catcagcagc acgagatagt
     3961 tcgtcgtgtg cagcacgaag ctcagataga cgcgggccgg attgctgttg ctgacgtcga
     4021 gaaacagccc ggcgaacagt aacaggacga taaacacgcc aaagccgtag agcacgcgat
     4081 tgcggatcgc ctcctgcacg gccaggcggg cgacggcaaa ggtgcggcgc ggcgaaaagc
     4141 ggggcaggtc ttcggtgacc gcggagaaca tcgcccgcgc gacgtagtaa aacgcctcgc
     4201 ttggcccgta tttgaacgaa gccacaacga agcagaaaaa cggcacgagg agcagcaaga
     4261 tgaagcaaat ggcaatcgtg ccgggcagaa agccccacga gtcgggccgg aacaacgacg
     4321 ccttgactgg atcgcccagc agccaggtcc aaaagttcgg aatgccgtct tccagtgcca
     4381 ttgtcagacc agatcaattg tcagaccaga tcaattgtca gaccagatca actgcggtgg
     4441 tgaatcggtt cagtgcttgc gccgctactt gtcgccggcg cggcggccgg gacgggcttc
     4501 gctgtcgcgg acgatgttca ggaacaattc ttccaacgac atcgtcggat gctcgaccgc
     4561 gattgtctgg cccccgtggc gggcgatgac ggcgcgaatt tcctcctccg cttccttgct
     4621 gaggccctga gcgcggaact gcgtcacgtc ctgaaccatc aacagctcat cgacgcgccc
     4681 cagctccttc aattccccct ggtgcaggat cgcgatccgg tcgcaaacgt cctgcacgtc
     4741 aggcaacagg tggctgcaca tcagaatcgt cttcccctgc tcgcgaagtt tgaggatcag
     4801 atccttcatc tcccgcgtgc cgatcgggtc caggcccgag gtcggctcgt cgagcaggat
     4861 cagctccggc tcgttgatca gcgcctgagc cagaccgatg cggcgggtca tgcccttcga
     4921 gtattcgcgg agctgccggc ggcgagccca gttgaggccg accatttcga tcagctcgtt
     4981 ggtccgctgc ttgcggacgg aagccggcat gtcgaacagc cgaccgtaaa aatcgagcgt
     5041 ctcctcagca ttgagaaact tgtagaggta cgactcttcc ggcaagtagc cgatccgctc
     5101 gttcttgctg acctccgtcg cttctttacc gaatacgagg gcctgtccct gggtgggaaa
     5161 caggaggccg agcaagagct tgatggtggt cgattttccc gagccatttg gtcccaggag
     5221 gccgaagatc tcgccgcggc ggacctgcaa gtccagagca ttgagcgccc gcaccttctg
     5281 ccgaccccag aaatcgcggt aaacctttgt cagatttcga gtttcgataa tgacttccga
     5341 cgaagggccg accgcttcgg ctttgatcgg ggcatcaagt tgggtggtca tgaatggcaa
     5401 agagggatcg aaaggctggt ccaagcggca agaagcagga ccggcgcggc gatttgcaag
     5461 gcttgacccg ccaagaactt tggcgctgca ggtgaactga ctacgcccca agcccggatt
     5521 cggttcaagg ggcgttgtaa tttctgcaag ggccgcaggc gggcacactc gctccgtgcg
     5581 actgctacaa cttcgcacta taaatggacg gctagcgagt tgacaagtct cggcgcaagt
     5641 ccaagcggct gaatgttttt cccactgcca cagcgatgat ttcgcagccc gtttccgagc
     5701 gagtaacgga actgtccgac tcccctgggg agtgaaagtc ttacaagaga atcgctcatg
     5761 aaggacacca cgcgccaatg gataatgatc ccggatttat tcgacggaaa gcgattccag
     5821 aattggactt gcgtcggttt atgagacgtg ctccgtcggc tggccctgtc gatccaagcc
     5881 ctttggcacg ctccttgctt aaagacaagt cagcgacgag gcggctgacg ttcagccgac
     5941 cctggatgga acccttggtc cttaagctcg ctgcaaggag gctgatgacc tgatgttttc
     6001 catttgccct gcggggcaaa tgcctacgcc gaccgcgatt gggtagagtc tcggccggcg
     6061 cgtttccc
//
LOCUS       DASUGB010000725         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_775204, whole genome shotgun sequence.
ACCESSION   DASUGB010000725 DASUGB010000000
VERSION     DASUGB010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775204"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFV87_13890"
     CDS             <1..755
                     /locus_tag="VFV87_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX5104905.1"
                     /translation="SIAMPAASDGPPPVPRRRFQFRLRTLLIAMLLVAVIVGLVTMQM
                     REARRQRKVVAHLQTLGARIGMHDSGSLMPALLDTRYLQRVHYVEMNGAPLKDAGVFA
                     DMMNLEELYLWGTEIDDAAPLSGLKKLRVLDLAQTKVRDIRPLTGLQNLKSLALAETP
                     VEDFASLRELRGLREFYSYKSTLDDLTPLASLEHLEVLNIYSTPVRDLSPLLGLKALH
                     SLDINDTAVSEDSVTRLRASLPNCQIRYGSGR"
     gene            complement(737..1885)
                     /locus_tag="VFV87_13895"
     CDS             complement(737..1885)
                     /locus_tag="VFV87_13895"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HEX5104906.1"
                     /translation="MRTCRSFLVAVAICAAGCAPTAKLAVLRPAQLDIGGIGRIAVVD
                     FQGEGETGAIARSAVLAKLTENRHYTIVDQAELARVQPAAFTEGMPAEAAAISAARQA
                     GVDAILTAQVVSYSAEDHVQQDSHFSIGGAGGGDRSGLRVGAMGLGFDTNTLHERDAS
                     VSLAFKLIDVRTGEIRDAQQVAHTYEGRVVNGRGEMPSRERILGNLLHHCSQDVVAAV
                     APHQEWVEVVLARQYWGNGLNALRRGNALAQSGDWAAAEEAWKSALERNPQNHAAHHN
                     LALACEARGDYAAAQVHLEKALNHFAAALYHQTRERLASESREHAAAQAQIASRPRML
                     PPANSLAPTTPGWPPPNASPPPNVIPAGHTSPADPPLERLPEIHRPDP"
     gene            2120..>2352
                     /locus_tag="VFV87_13900"
     CDS             2120..>2352
                     /locus_tag="VFV87_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104907.1"
                     /translation="MLAPRLQQLAIVVLVAATVMLTNLGGPRLWDRDEPRNAGCAREM
                     LQRGDWIVPTFNGELRAQKPVFLYWCIMAGYSV"
BASE COUNT          389 a          736 c          776 g          451 t
ORIGIN      
        1 cgtcgatcgc catgccagcc gcatctgacg gcccacctcc ggttccgcgc cggcgatttc
       61 aattccggct gcggactttg ctgatcgcca tgctgctggt ggcggttatc gtcggcctgg
      121 tgaccatgca gatgcgcgaa gcccggcggc agcggaaggt agtggcgcac ctacaaacac
      181 ttggagcgcg catcggaatg cacgattcgg gctcgttgat gcccgcgctg ctggatacgc
      241 gctatctcca gcgcgtgcat tacgtggaga tgaatggagc gccgctgaag gatgctgggg
      301 tatttgccga catgatgaat ctggaggaac tctacctctg gggtacggaa atcgatgacg
      361 cagctccact tagtggcttg aagaagttgc gcgtgctcga tttggcccag accaaggtcc
      421 gggacattcg tcctctgaca gggctgcaga atctgaaatc gcttgccttg gccgagactc
      481 ccgtagaaga cttcgcatcg ctgcgtgagt tgcgtggcct gcgagagttc tattcgtaca
      541 agtcaacgct tgacgatctg acgccgctcg cgtcgcttga gcatctggaa gtcctgaata
      601 tctatagcac gcccgtccgc gatctctcgc ccctgcttgg tctgaaggct ctccattcgc
      661 tcgacatcaa tgatacggcg gtatccgaag attcggtcac gcggctgcgc gcatcgcttc
      721 ccaattgcca gatccgctac gggtctggcc ggtgaatctc cgggaggcgc tccagcggcg
      781 gatcggcagg cgacgtatgg ccggccggga tgacgttggg aggcggagag gcatttggtg
      841 gaggccaacc aggcgttgta ggcgccagcg agttcgccgg cggcagcatc cgcgggcgcg
      901 acgcgatttg ggcctgggcc gcggcgtgct cgcgactttc agacgcaagc cgctcgcgcg
      961 tctggtgata gagcgcggcg gcaaagtgat tcagggcttt ttcgaggtgg acctgggctg
     1021 cggcgtagtc gccgcgggcc tcacacgcca aggccagatt gtgatgggcc gcgtggttct
     1081 gcggattccg ctccaaggcc gatttccagg cctcttccgc ggcagcccag tcgccggact
     1141 gcgccagggc gttgccgcgg cgaagggcgt ttaagccgtt gccccagtac tggcgggcga
     1201 ggacgacttc cacccattcc tgatgcggag ccaccgccgc gacaacgtct tgcgagcaat
     1261 ggtgaagcag attgccaagg attcgctcgc ggctgggcat ctcgccgcgg ccgttgacga
     1321 cgcgtccttc gtaggtatgg gcgacctgct gggcgtcgcg aatttcgccc gtgcgaacgt
     1381 cgatcagctt gaaggccagc gaaacggacg cgtcgcgctc gtggagcgta tttgtatcaa
     1441 atccgagccc catcgctccc acgcgcaagc cgctgcggtc cccgccaccg gcgccgccga
     1501 ttgaaaagtg gctatcctgc tggacgtggt cctccgcact gtaactcaca acctgcgcgg
     1561 tcaggatcgc atcgacgccc gcctgccggg cggccgagat cgcggcggcc tcggcgggca
     1621 ttccctccgt gaaggcggcc ggctgaacgc gggcgagctc ggcttgatcg acgatcgtgt
     1681 agtgccggtt ttcggtcagc ttggccagca ccgcggaacg ggcgattgcg cccgtttcgc
     1741 cttcgccttg aaaatcgacg accgcgatcc ggccgatgcc gccgatatcg agctgggctg
     1801 gccgcaggac ggcgagtttg gcagtcggtg cgcagccggc agcgcagatg gcgaccgcca
     1861 ccaggaacga ccggcaggtg cgcattgcaa agcctttcgc gctggctcaa gttttcgcgc
     1921 ccaagtcccg caatcggggc gggatgtaca ccagccgggt tgatctgggc aagagcagtc
     1981 gcggtcgggt attcttcgcg gccaccgtag gctactcgct ccgcgagtag caatccgcct
     2041 accattggcg cccggcctgt ccagcccagt tcgcttatcg catctcgcag agcgagatgc
     2101 ctacgatgag gttcccttcg tgcttgcacc ccgcctccag cagctagcga tcgtggtcct
     2161 tgtcgccgcg acggtgatgc tcaccaacct cggcggaccg cgcctgtggg accgcgatga
     2221 gccacggaac gcgggctgcg cgcgggagat gctccagcgc ggcgactgga tcgtgccgac
     2281 cttcaacggc gaactccgcg ctcagaagcc ggtgttcctg tattggtgca tcatggcggg
     2341 gtacagcgtc tt
//
LOCUS       DASUGB010000726         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1468792, whole genome shotgun sequence.
ACCESSION   DASUGB010000726 DASUGB010000000
VERSION     DASUGB010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468792"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..568
                     /locus_tag="VFV87_13905"
     CDS             227..568
                     /locus_tag="VFV87_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104908.1"
                     /translation="MPQPKPTNPFYLALIPVGVIFGITACSYMVMAYRGLSPHNESEL
                     GLPGLMNHWGMAIMGVELGLLAVLTVAAIGSDDFWTRRFAAAQNPTPEGEREASRSEN
                     GNLEGVGSQLR"
     gene            631..1014
                     /locus_tag="VFV87_13910"
     CDS             631..1014
                     /locus_tag="VFV87_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5104909.1"
                     /translation="MKVSPYQATPQQPVEMEGSHGCQVRWLLGERDGTPNFAMRQFEV
                     APGGHTPRHSHPYEHEVFVLEGEGVVFEGDQPHPLKAGDVVFVKPDEVHQFRNPGSSP
                     LKFLCLIPNSAAGKKVTVAPECGRE"
     gene            1298..1609
                     /locus_tag="VFV87_13915"
     CDS             1298..1609
                     /locus_tag="VFV87_13915"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX5104910.1"
                     /translation="MLPHPIVRVTACTQVGPFTLRVEFDDGAAQTIDFESVLYGGIYE
                     PLRDPAFFAQYRIDPDFHTLVWPNDADFDPATLRHWPEYLPHLLEMVERWKATPSIVQ
                     S"
     gene            1680..>2056
                     /locus_tag="VFV87_13920"
     CDS             1680..>2056
                     /locus_tag="VFV87_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5104911.1"
                     /translation="MPSPAQRIEQLREEIRGHDYRYYVLARPTISDREYDKRMQELID
                     LERQHPELATPDSPTQRIGGDVQTELKPVRHAVPMMSIDNTYSEAEIRAFDERVRKLL
                     EGDEPAYVLEPKIDGTSINLRYE"
BASE COUNT          409 a          624 c          639 g          384 t
ORIGIN      
        1 gccaccagct tgtggtgcgg aatgtcgagg tccacctgcc tgaggttgtg gacgctgacg
       61 ccgcgaagct cgatctggcg gctggtcacg ctcggctgct cccgcggagg atttcgacaa
      121 tgggccaatc tggcagttta acgtcgtagg acaggtttcc aacccgtccg cgttcgcacg
      181 gacggcttgg aaagccgtgc tacaaacgcc ttgctataat tcagcgatgc cccagccgaa
      241 gccgacgaac ccgttctatc tggcgctgat cccggtgggg gtgatcttcg ggatcaccgc
      301 ctgctcgtac atggtgatgg cctatcgtgg cctcagcccg cacaacgaga gcgagctcgg
      361 tctgcccggg ctgatgaacc actgggggat ggcgatcatg ggcgtcgagc tggggctgct
      421 cgcggtcctc accgtcgccg ccatcgggag cgacgacttc tggacgcggc ggtttgcggc
      481 ggcgcagaat ccaacgccag aaggcgagcg agaggcatcg cgttctgaga acggcaacct
      541 ggaaggggtc gggagtcaat tgcggtgacg atcatccaag tggaaagtgg cagccgcaat
      601 tgactcccga ccccttgagg acgaatcacc atgaaagtct ccccctacca agcaactccc
      661 cagcagccgg tcgaaatgga aggctcccac ggctgccagg tccgttggct cctcggcgag
      721 cgcgacggca cgccaaattt cgcaatgcgg cagttcgagg tcgcccccgg cggccatacg
      781 ccgcgccata gccatcctta cgaacacgaa gtcttcgtcc tggaaggcga aggagtcgtt
      841 tttgaaggcg accagcctca cccgctcaaa gccggtgatg tggttttcgt caagccggac
      901 gaagtgcatc agttccggaa cccgggcagc tcgccgctca agttcctgtg tttgattccg
      961 aactccgcgg cggggaagaa ggtgacggtc gcgcccgagt gcgggaggga ataattcggg
     1021 agaggtagga tgccagaact gtcgcgcttt cacggcatca ttgttaggat gttttgggag
     1081 cctcaagccc ctcatcatcg tccgcatttt catgccgtct attacggaag cgcagtctcg
     1141 atggccattg atacaataga agtgttagct gggggcttgc cgagaaggca gcagcggctc
     1201 ctggaggcct gggccgaatt gcaccaccag gagttgctcg acgcctggct agccatgcaa
     1261 atacgacaac cctttcgaaa aatcgatccg atctaacatg ctcccccatc ccatcgttcg
     1321 tgtgacggcg tgtacgcaag tcggcccctt taccttgcga gtggagttcg acgacggcgc
     1381 cgcgcaaact atcgactttg agtccgtgct gtacggcggc atctacgaac cgcttcgcga
     1441 tcccgcgttc tttgcgcagt accgaatcga tcctgacttt cacacgctcg tctggccgaa
     1501 cgatgccgat ttcgacccag ccacgctgcg gcattggccc gagtatctgc cgcacctgtt
     1561 ggagatggtc gagcggtgga aggcgacgcc ctcgatcgtg cagtcgtaag gcagttttgt
     1621 cggagtcgtg gatggggcgt gcgactgcgt tcgatgggat cagaaaacta atcgactcta
     1681 tgccctctcc tgcccagcgc atcgaacagc tccgcgagga aatccgcggg cacgattatc
     1741 gctattacgt cctggcccgc ccgacgatca gcgaccgcga gtacgacaag cggatgcagg
     1801 agctgatcga cctggagcgg cagcatccgg agctggccac gcccgattcg cccacgcagc
     1861 ggatcggcgg cgacgtgcag accgagctca agccggtccg gcacgccgtg ccgatgatga
     1921 gcatcgacaa cacgtacagc gaggccgaaa tccgggcctt cgacgagcgg gtccgcaagc
     1981 tgctggaagg cgacgagccg gcctacgttc tggagccgaa gatcgacggc acgtcgatca
     2041 acctgcggta cgagca
//
LOCUS       DASUGB010000727         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_775235, whole genome shotgun sequence.
ACCESSION   DASUGB010000727 DASUGB010000000
VERSION     DASUGB010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775235"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VFV87_13925"
     CDS             <1..1178
                     /locus_tag="VFV87_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104912.1"
                     /translation="KLIQWGVLGELHHIRAQWHRGNLPGADSWQPPLPGGERSWSDGE
                     WKTVDRIAQQLKGYQSQLKNATDPTEAALLEKKVAQWEAWTKDAEVEAQNYGYQDFDL
                     GDGRTRSPLEELIRWRLWQRTGGGLMAELGSHQLDACSIFCSALRAKELGGDGKKAHP
                     LTVHAVGGRHTFPLDRDAEDHVYCTYEFPGPGYEPKFPVGYYDRAMNYPDPKTGIPPY
                     KDDKNKKIVVTYSSINGNGFGGYGETVMGTRGTLVLEREQEVLVYKDSDTTTRVGVKD
                     DKGGPTLDTQASGKGPALAKAAAATGPVSRGYTEEIEHWAWCIRNPSSENQPRCRPEV
                     ALGDAVIALATNVAMDNAKSGKGGYLVFDEKWYDLADDATPDGSDVKTTAERLGANI"
     gene            1240..2085
                     /locus_tag="VFV87_13930"
     CDS             1240..2085
                     /locus_tag="VFV87_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5104913.1"
                     /translation="MEKWPLGVFASVDAGLGVKLEVARELGVPTIQLHAPHASTRTKA
                     NADAFLKRIADYGIKLTCVFGGFTGESYADIPTVSKSVGLVPPQTRAARTKELKEIAD
                     FAHLLGCKCVGLHIGFVPHDPADPLYQQIVAVARDVCDHLAKNGQALHLETGQETADS
                     LLQFIQDVERPNLFINFDPANMILYGTGEPIAALKKVGRYVRSVHCKDGKWAELAGKE
                     WGQEVPLGEGDVGMENYLRTLKEIGYDGPLTIEREIPQEPERQKAEISHAIKLLTELR
                     KKVLV"
     gene            complement(2183..>2433)
                     /locus_tag="VFV87_13935"
     CDS             complement(2183..>2433)
                     /locus_tag="VFV87_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104914.1"
                     /translation="SWIAVRVFPSSHTNPVFVEVDGQPIRASKRSAQWCLDAVETCWN
                     QKVKQTRETEKTAARAAYDVARDAYAKVLAEAFDDRPK"
BASE COUNT          480 a          784 c          800 g          369 t
ORIGIN      
        1 tcaagctgat ccagtggggc gtgctgggcg aattgcacca tattcgcgct cagtggcacc
       61 gcggcaacct gccgggcgcc gacagctggc aaccgccact tcccggcggc gagcgctcgt
      121 ggtcggatgg cgagtggaaa accgtcgatc gcatcgccca gcagctcaag gggtaccagt
      181 cgcagctcaa gaatgccacc gacccgaccg aggcggccct gctcgaaaag aaggtcgccc
      241 agtgggaggc gtggacgaag gacgcggagg tcgaggccca gaactatggc taccaggatt
      301 tcgacctggg ggacggccgc acccgctcgc ccctggagga gctcattcgc tggcggctct
      361 ggcagcgcac cggcggcggc ctgatggcgg agctgggcag ccatcagctc gacgcgtgca
      421 gcatcttctg ctcggccctg cgagcgaagg agctgggggg cgacggcaaa aaggcccatc
      481 cgctcacggt ccacgcggtg ggcgggcggc acaccttccc gctcgaccgc gacgccgagg
      541 accacgtcta ttgcacctac gagttccccg gcccgggcta cgaacccaag tttccggtcg
      601 gctactacga ccgggcgatg aactaccccg atccgaaaac gggcatcccg ccgtataagg
      661 acgacaagaa taagaaaatc gtcgtcacct actcgtcgat caacggcaac ggattcggcg
      721 gctatggcga gacggtgatg gggaccaggg ggacgctcgt gctggagcgc gagcaggagg
      781 tgctcgtcta taaggacagc gacacgacga cgcgcgtcgg cgtgaaagac gacaaggggg
      841 gaccgacact cgacacccag gccagcggca agggtccggc gctcgccaag gcggccgccg
      901 ccaccggccc ggtcagccgc ggctataccg aggagatcga gcactgggcc tggtgcatcc
      961 gcaacccgtc atccgaaaac cagccccgct gccggccgga agtggcgctg ggagacgcgg
     1021 tcatcgccct ggcgacgaac gtggccatgg ataacgccaa gagcggcaag ggcggctacc
     1081 tggtgttcga cgagaagtgg tacgatctgg cggacgatgc cacgcccgac ggcagcgacg
     1141 taaagaccac agccgagcgc ctcggcgcaa acatctgacc gccgacaact tcgtcctctg
     1201 tccactgtcc tctgtctcct aactcctcgg agcctcccca tggaaaagtg gcccctcggt
     1261 gtatttgcca gcgtcgatgc aggactgggc gtcaagctgg aagtcgcccg cgagctgggc
     1321 gtgccgacga tccagcttca tgccccgcac gccagcacgc ggacgaaggc caacgccgac
     1381 gccttcctga aaagaattgc cgactacggc atcaagctga cctgcgtttt cggcggcttt
     1441 accggcgaga gctatgccga catccccacg gtgtcgaaat cggtcggcct ggtgccgcct
     1501 cagactcgcg ccgcccgcac caaggagttg aaggagattg ccgacttcgc ccacctgctg
     1561 ggctgcaaat gcgtcggtct gcacatcggc tttgtgccgc acgatccggc cgatccgctt
     1621 taccagcaga tcgtggccgt ggcccgcgac gtgtgcgacc acctggcgaa aaacggccag
     1681 gcgctgcacc tggaaacggg gcaggaaacg gccgattcgc tgctccaatt cattcaagat
     1741 gtcgagcggc cgaacctgtt catcaatttc gacccggcca acatgatcct gtacggcacg
     1801 ggcgagccga tcgccgccct gaagaaggtg ggccgctacg ttcgcagcgt ccactgcaag
     1861 gacggcaagt gggccgagct ggccggcaag gagtgggggc aggaggtgcc gctgggcgaa
     1921 ggggatgtcg gcatggagaa ctacctgcgg acgctcaagg agatcggcta cgacggcccg
     1981 ctcacgatcg agcgcgaaat ccctcaggaa cccgagcggc aaaaagccga gatcagccac
     2041 gcgatcaagc tgctgaccga gctgcggaag aaggttctgg tttagcgggc ccttagccgg
     2101 gaccaaactg gtcccggcga accgcgagtt gataccttcc atcgcgacgc tccgggagaa
     2161 cgtttgctcc cggctaagaa cactatttgg gccggtcgtc aaacgcttcc gccaaaactt
     2221 tggcgtacgc atcgcgggcg acgtcgtagg cggcgcgggc ggcggtcttt tcggtctccc
     2281 gggtttgctt gaccttctgg ttccagcagg tctcgacggc gtccaggcac cactgggcgc
     2341 tccgcttgct ggcgcggatc ggctggccat cgacctcgac gaacacggga ttggtgtgcg
     2401 agctggggaa gacgcgaacc gcgatccaac tcg
//
LOCUS       DASUGB010000728         6249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1747825, whole genome shotgun sequence.
ACCESSION   DASUGB010000728 DASUGB010000000
VERSION     DASUGB010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747825"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2401
                     /locus_tag="VFV87_13940"
     CDS             <1..2401
                     /locus_tag="VFV87_13940"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5104915.1"
                     /translation="PQPLAAQAKRIAEALAYLGEPLSKEQQAALEKAASNMKPEESVE
                     AIQKVLDPRCLAGVNINPESRVKVARGPAAAELAEQGWRVFLVKVHNEAGVTAALRAS
                     SPNAAPLHTRSTSSPAPKTTVKPEDVPIRWMDITTFDSQPLSPKLSGLELEYRIVQIY
                     SRDKGKREATIGFDVGQGTQDIGFRNEIAVLFECEPAVRVKLSILDDDGTPTTGQLTF
                     RDKLGRVYPSRSRRLAPDFFFHDQIYRHDGEDVILPPGEYEVTYNRGPEYLVLKKSIT
                     VPAAAEHSESFRFKRWIKLTDLGWYSGDHHVHAAGCAHYEAPTEGVTPADMMRHILGE
                     DLNIGCVLSWGPCWYHQKQFFEGQVHKLSTKDYVMRYDVEVSGFPSQHAGHLCLLRLK
                     EDDYTYPQEAEFDWAFSGQNGHFKGTKTEFIGEWPTWDLPILKWGKEQGGVVGFSHSG
                     WGLALPDYGPNGERVPFPGRNPQPGVTTRAADKLPDYAMPPFDGIGANEFVVDTVHGV
                     CDFISSVDTPAVWELNVWYHTLNCGMTTRISGETDFPCIYGDKVGLGRIYVKLDKAAT
                     SGRGSPDPKAATSARGSDQLDFDSWCEGIRDGRSYCCDGLSHLIDFSVNGLGVGEKGD
                     KDRASFLAAKSGDKLTIKVNAAAFLNEEPNTWYGTPIKQIRLDQKPYWHVERARIDGT
                     RKVPVELIVNGHAVEKQEIDADGRINELTFNYTPERSSWVAIRIFPSSHTNPVFVEVD
                     GQPIRASKKSAQWCIDAVETCWNQKVKGTRESEKAAARAAYDVARDTYKKVLAEAFDD
                     R"
     gene            2398..2769
                     /locus_tag="VFV87_13945"
     CDS             2398..2769
                     /locus_tag="VFV87_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104916.1"
                     /translation="MKHYQLASAAMNRSALVFIAGLVVGFGLGCCLMLLVSQPTPSAK
                     AVPAAIARRDPKAPIVRTSPQALLDVVNQRPESVSALLITFGGSSVELKSGVRLMAET
                     VEQQQELQDAARKAGVPFAIE"
     gene            complement(2781..3668)
                     /gene="dapA"
                     /locus_tag="VFV87_13950"
     CDS             complement(2781..3668)
                     /gene="dapA"
                     /locus_tag="VFV87_13950"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5104917.1"
                     /translation="MPRKGSAFAGLSVAIVTPFQDGQVDVKRLREQVEFQVAAGTQCL
                     CPVGTTGESPTLSHEEHERVISEVVQAARGRIKVMPGTGSNSTAEALRLTKWAAKEGA
                     DAALMVAPYYNKPTQEGFFLHYKAVAEQVGIPLCVYNIPGRTGKNIEPETIARMAELP
                     NITLVKEATGSLDQASQILCTTDLTVLSGDDSLTLPMMSVGGEGVISVVGNIVPRDML
                     AMTTAYLQGEVAAAMRWHHKLFPLCRDTLGLATNPIPIKAAMKLLGRDTGELRLPMTP
                     LDSASEAKLRKTLTGYGLL"
     gene            3760..4404
                     /locus_tag="VFV87_13955"
     CDS             3760..4404
                     /locus_tag="VFV87_13955"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5104918.1"
                     /translation="MRWFAALLVLACAACSIRAEDTNGFRPLFDAKTLDGWVNKTEDG
                     QPNADNKWTVKDGVLTAAAGSGWLSTKEMYGDFVLKLQWRVPENGNSGVFIRIPDLKP
                     GQHPHIDGIEIQVLDDKGPQYEGKLQPYQYSGSIYGAVPAKDSGYKGAGEWNSFEITC
                     RGEKISVVMNGNRVAEADVTKIDMLSQRPRRGYIGLQNHGSGVEYRNVELKVLE"
     gene            4550..5206
                     /locus_tag="VFV87_13960"
     CDS             4550..5206
                     /locus_tag="VFV87_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104919.1"
                     /translation="MASWLLVLAAACGAEGAESGARGELRGLHEYHNQISDLFKREVQ
                     AKEPGTRAVVIHEMCDLHREIVSDERFTTSDKLKEYRGKLWSRLSKIKTELKQQLARE
                     AKTNKQPLEDVALLESADPAAVAAADSLASSLSLLDQTQGGPGYLLGYGGRAQIDANG
                     QSLIELIERTINPAFWDVVGGPGTIMYYAPLQCLVVRATSEIHGNVGRLADDLRAAGP
                     "
     gene            complement(5323..>6249)
                     /locus_tag="VFV87_13965"
     CDS             complement(5323..>6249)
                     /locus_tag="VFV87_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX5104920.1"
                     /translation="RDTVRRFWKGDGGLASELATRLAGSSDLYQDDGRHPSASVNFIT
                     CHDGFTLRDLVSYNEKHNEANGENNGDGANDNDSWNCGEEGPTADPLVSELRQRQMRN
                     FLTTLLLSQGVPMLLAGDETGHSQQGNNNTYCQDNELSWIDWQLTPERQSLLDFARKL
                     VEIRRNQPALQRRRFFHGQPIFGRSVKDIYWLDNSFQEMTDEAWSAGFVKALGMVLVG
                     ANGELDAKGEPIVGDSLMLLLNAYWEPIEFQFPKLIGIVTDFTRLFDTAMPAAPPSPV
                     NLNHAYSLHPRTTALFRWMPRVILNNGVAPKV"
BASE COUNT         1167 a         1925 c         2055 g         1102 t
ORIGIN      
        1 gccgcagccg ctggccgcgc aggccaagcg gatcgccgag gccctggctt atctgggcga
       61 accgctctcg aaagagcagc aggccgcatt agagaaggcc gcgagcaaca tgaagccgga
      121 ggagtcggtg gaagcgattc agaaggtgct cgatccgcgc tgcctggcgg gcgtgaacat
      181 caaccccgag agtcgcgtga aggtcgcccg cggcccggcc gcggccgaac tggccgagca
      241 gggctggcgg gtctttctgg tgaaggtaca caacgaggcg ggtgtcactg cggctctgcg
      301 ggcgtccagc cccaatgcgg ccccgctgca cacccgctcg accagcagcc cggcgcccaa
      361 gacgaccgtc aagcccgagg acgtgccgat ccgctggatg gacatcacga ccttcgacag
      421 ccaaccgctg tcgcccaagc tctcgggcct ggaactcgag taccgcatcg tccaaattta
      481 tagccgcgac aaaggcaagc gcgaggcgac gatcggtttc gatgtcggcc aggggacgca
      541 agatatcggg tttcgtaacg agatcgccgt cctcttcgag tgcgagccgg ccgtgcgggt
      601 aaagctctcg atcctggacg acgacggcac gccgacgacc gggcagctca cattccgcga
      661 caagctgggg cgggtgtatc cctcgcggtc gcgccgcttg gcgccggact tcttcttcca
      721 cgaccagatc tatcgccacg atggcgagga tgtgatcctg ccgccgggcg aatacgaagt
      781 gacttataac cgcggaccgg aatacctcgt gctgaagaag tcgatcaccg tccccgccgc
      841 cgccgagcac agcgaatcgt tccgcttcaa acggtggatc aagctgaccg acctgggctg
      901 gtattcgggg gaccatcacg tccatgcggc cggctgtgcc cactatgagg ccccgaccga
      961 aggcgtgacg cccgccgaca tgatgcggca catcctgggc gaggacctca acatcggctg
     1021 cgtcctttcg tgggggccgt gctggtatca ccagaagcaa ttcttcgagg ggcaggtcca
     1081 caaactctcg acgaaggatt acgtgatgcg gtacgacgtg gaagtctccg gcttccccag
     1141 ccagcacgcc gggcatctgt gcctcctgcg tctgaaggag gacgactaca cctacccgca
     1201 ggaggccgaa ttcgactggg ccttcagcgg ccagaacggc catttcaagg ggaccaagac
     1261 ggagttcatc ggcgagtggc cgacctggga cttgccgatt ctcaagtggg gtaaggagca
     1321 aggcggtgtc gtcggcttct cgcatagcgg ctgggggctg gcgctgcccg attacggacc
     1381 caatggtgag cgcgttccgt tccctggccg caatccgcag ccgggcgtga caacgcgtgc
     1441 agccgacaag ctgcccgatt acgccatgcc tccattcgac ggcatcgggg cgaatgaatt
     1501 cgtcgtcgat acggttcacg gcgtgtgcga tttcatttcg tcggtcgata cgccggcggt
     1561 ctgggagctg aatgtctggt atcacacgct caactgcggc atgacgacgc ggatcagcgg
     1621 cgagaccgat ttcccgtgca tttacggcga taaagtcggc ctcggtcgca tctacgtgaa
     1681 gctcgacaaa gccgcgacca gtggtcgcgg ttcccctgac cccaaagccg caaccagtgc
     1741 tcgcggttcc gaccagctcg acttcgattc ctggtgcgaa ggcatccgcg acggccgctc
     1801 gtattgctgc gacggcctct cgcacctgat cgacttctcg gtcaatggcc tcggcgtcgg
     1861 cgagaagggg gacaaggacc gggcgagctt cctcgccgcc aaatccggcg acaagctgac
     1921 gatcaaggtc aacgccgccg ccttcctcaa cgaagagccc aacacctggt acggcacgcc
     1981 gatcaagcag atccgcctcg accagaagcc ctactggcac gtggagcggg cccggatcga
     2041 cggcacgcgc aaggtgccgg tcgagctgat cgtcaacggt cacgccgtcg aaaagcagga
     2101 gattgacgcc gacggacgga tcaacgagtt gacgttcaac tacacgccgg agcgttcgag
     2161 ctgggtggcg atccgcattt tccctagctc gcacaccaac ccggtcttcg tcgaagtcga
     2221 tggccagccg atccgcgcca gcaagaagag cgcccagtgg tgcatcgacg ccgtcgagac
     2281 ctgctggaac cagaaggtca aaggcacgcg cgaatccgaa aaagccgccg cccgcgccgc
     2341 ctacgacgtc gcccgggaca cctacaagaa ggttttggcc gaggcgtttg acgatagatg
     2401 aagcactatc agttggcgag cgcggctatg aacagatcag cgttggtatt catcgcgggt
     2461 ctcgtggtcg gtttcggatt aggatgttgc ctcatgttgt tggtcagcca accaactcct
     2521 tcggcaaagg ctgtcccggc agccattgcg cggcgtgatc ccaaagcgcc aatcgtgagg
     2581 acctcgccac aagcgctatt ggacgtggtc aatcagcggc cggagagcgt ctctgcgctg
     2641 ctgattacgt ttggagggtc gtcagtggaa ttgaagagcg gcgtccgctt gatggcggaa
     2701 acggtggagc aacagcagga attgcaggat gccgcgcgaa aggcaggagt gcccttcgca
     2761 atcgagtgag ctcgcggtcg ctacagcaac ccataccccg tcagcgtctt tcgcagcttc
     2821 gcttcgctcg cggaatccag cggcgtcatc ggcaggcgga gctcgccggt gtcgcggccg
     2881 agcagcttca tcgcggcttt gatggggatg gggttggtgg ccaggccgag cgtgtcgcgg
     2941 cagaggggga agagcttgtg gtgccagcgc attgccgcgg cgacttcgcc ctggagataa
     3001 gccgtggtca tcgccagcat gtcgcgggga acgatgttgc cgacgaccga aatgacccct
     3061 tcgccgccca ccgacatcat tggcagcgtc aggctgtcgt cgccgctgag gaccgtcagg
     3121 tcggtcgtgc agagaatctg cgacgcctgg tcgagcgagc cggtcgcttc cttgaccagc
     3181 gtgatgttcg gcagctcggc catccgggcg atcgtttccg gctcgatgtt cttgcccgtg
     3241 cggcccggaa tgttatagac gcagagcgga atgcccacct gctcggccac cgccttgtag
     3301 tgcaggaaga agccctcctg cgtcggcttg ttgtaatagg gggcgaccat cagggccgca
     3361 tcggctcctt ccttggccgc ccacttcgtc agccgcaggg cttcggctgt gctgttgctg
     3421 ccagtgccgg gcatgacctt gattcgtccg cgggcggcct ggacgacctc gctgatgacc
     3481 cgctcgtgct cttcgtgcga gagcgtgggc gattcgccgg tcgtccccac cgggcagagg
     3541 cactgcgtcc ccgcggcgac ctggaattcg acctgctcgc ggagccgctt cacatcgacc
     3601 tgcccgtcct gaaacggcgt gacgatggcg accgacaacc cggcgaacgc agaacctttg
     3661 cggggcataa acaacctgca cagagcggga gacttatcga tctgccataa tacgtgacgc
     3721 taaccacgac cgaaacccgc caagccctgg agaactacca tgcgatggtt tgccgctttg
     3781 ctcgtcctcg cctgtgcggc ctgctccatt cgcgctgaag atacaaatgg atttcggcca
     3841 ctctttgacg cgaagacgct cgatggctgg gtcaacaaga ccgaggacgg ccagccgaac
     3901 gcggacaata agtggacggt gaaggacgga gtgctgactg cggcggccgg cagcggctgg
     3961 ctgagcacga aggagatgta cggcgatttc gtgctgaagc tccagtggcg cgtgccggag
     4021 aacgggaaca gcggggtctt cattcgcatt cccgatctca agcccggcca gcacccgcac
     4081 attgacggaa tcgagatcca ggtgctcgac gacaaaggcc cgcagtacga aggcaagctg
     4141 cagccgtatc agtattcggg ctcgatctac ggcgcggtcc cggccaagga ctcaggctat
     4201 aagggggccg gcgagtggaa cagcttcgag atcacctgcc ggggtgagaa aatcagcgtc
     4261 gtcatgaacg gcaaccgggt cgccgaggcc gacgtgacga agatcgatat gctcagccag
     4321 cgtccccgcc gcggctacat cggcctgcag aaccacggca gcggggtgga atatcggaac
     4381 gtcgagctga aggtgctgga gtaggcgggg cagacattct tgtctgccgg caggcaaaat
     4441 gcctgccccc cgttaaagat tgctttgctt gccaccgcgg agtcgataca ttgagtagtg
     4501 cgtctgtcgc tgggggcagc gggcgggcgg tggtgaggag gcgactctca tggcgagttg
     4561 gctattggtg ttggcggcgg cctgtggggc cgaaggtgcg gaatccggcg ctcgcggcga
     4621 actccgcggg ctgcacgaat atcacaacca gatcagcgat ctcttcaagc gcgaggtgca
     4681 ggcgaaagag ccgggcacgc gggcggtcgt gatccatgaa atgtgcgatc tgcaccgcga
     4741 gatcgtgagc gacgagcggt tcacgaccag cgacaagctg aaggaatacc gcggcaagct
     4801 ctggagccgg ctgtcgaaga tcaagaccga gctcaaacag caactggccc gcgaggcgaa
     4861 gaccaacaag cagccgctgg aggatgtggc gctgctcgaa tcggccgatc cggcggcggt
     4921 cgcggcagcc gatagtttgg cttccagctt gtctctcctc gaccaaacgc agggcgggcc
     4981 cggctatttg ctgggctacg gcgggcgggc ccagatcgac gccaacgggc aatcgctgat
     5041 cgagctgatc gagcggacga tcaatccggc gttttgggac gtcgtcggcg gcccgggcac
     5101 gatcatgtat tacgccccgc tgcagtgcct cgtcgtgcgg gctacgtccg agatccacgg
     5161 caacgttggc cggctggcgg acgacctgcg ggccgccggg ccgtagttcg ctgtagctgc
     5221 accctccaga gtgcggcagc gcgccgataa cccgcattct ggcgaatgcg gctacgaatg
     5281 ccggtgggcc tgcgctgcgc tggtcccacc ctacatctac acctacacct tcggcgccac
     5341 gccgttattg agaatcaccc gcggcatcca gcgaaacagg gcggtggtgc gcgggtgcag
     5401 cgagtaggcg tggttcaagt tcactggcga ggggggcgcg gcgggcatcg ccgtgtcgaa
     5461 taagcgcgtg aagtcggtga caatgccgat cagcttcggg aactgaaact cgatcggctc
     5521 ccaataggcg ttgagcagca gcatcaggct gtcgccgacg atcggctcac ctttggcgtc
     5581 gagctcgccg ttggcgccga cgagcaccat tcccagggcc ttgacgaagc ctgcgctcca
     5641 ggcctcgtcg gtcatttcct gaaagctgtt gtcgagccag tagatgtctt tcacgctccg
     5701 gccgaagatc ggctggccgt ggaagaaccg ccgtcgctgc agggcgggct gattgcggcg
     5761 gatttcgacg agtttgcggg caaaatcgag cagcgactgg cgttcgggcg tgagctgcca
     5821 gtcaatccac gacagctcat tgtcctggca ataggtgttg ttgttccctt gctgtgaatg
     5881 gccggtttcg tcgccggcca ggagcatcgg cacgccctga gagaggagca gcgtcgtcag
     5941 gaagttgcgc atttgccgtt ggcgcagctc gctcaccagc gggtcggcgg tcggcccttc
     6001 ctcgccgcag ttccaggagt cgttgtcgtt ggccccgtcg ccgttgttct cgccgttggc
     6061 ctcgttgtgc ttctcgttgt agctgaccag gtcgcgcagc gtgaagccgt cgtgacaagt
     6121 gatgaagttg acgctggcgc tgggatggcg gccgtcgtct tgatacaggt cgctgctgcc
     6181 ggccagccgc gtcgccagct cgctcgccag cccgccgtcc cccttccaga agcggcggac
     6241 cgtgtcgcg
//
LOCUS       DASUGB010000729         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1330530, whole genome shotgun sequence.
ACCESSION   DASUGB010000729 DASUGB010000000
VERSION     DASUGB010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330530"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..632)
                     /locus_tag="VFV87_13970"
     CDS             complement(351..632)
                     /locus_tag="VFV87_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5104921.1"
                     /translation="MAFVVTRNCFGCKYTDCVVVCPVEAFREGEQMVYIDPDECIDCG
                     LCLPECPVEAIFYEDDVPADQREFIALNAEMAKVCPPIVERREPLAARE"
     gene            760..2244
                     /locus_tag="VFV87_13975"
     CDS             760..2244
                     /locus_tag="VFV87_13975"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104922.1"
                     /translation="MSREEVNSLSSADLLRRLHAGDEAAAEAVFERYADRLTRLAQSR
                     LTVRLASRIDPEDIVQSAYRSFFVAARAGRFRVRSGGDLWRLLVEVTLHKLYRQAAHH
                     GAQRRSVAREVRIDDSASSAGVPTGGEPTPDEALAAAEELEAIFAQLSDRVRQALELR
                     LQGYEHEEIAERLACSERTVRRSLAEARRVMAARAGGGFVPSAARRRAVPTPSPAIPA
                     APIEIASPIPWSDLVLQQQIGAGATGKVYRALRRSTGAEVAVKFLRKSLLREPAVIER
                     FLREAKTVAQIAQPGIVGMHGAGRTPSGGYFLVMDLIGGGDLDRLRRCQPVSPPDAAA
                     WVAQAARIVHSAHEQGVVHCDLKPGNLLLDEQGQIHVTDFGLAARAAAGESNLLAGTP
                     AFMAPEQVDPAWGPISPRTDIFGLGAVLFFLLFGQPPHTGATIAEVLSRVAADSPVAI
                     PDQRDDRLNLDLVAIIRRCLAKSPAGRFPTAAELAAGLRTCGVA"
     gene            2566..3897
                     /locus_tag="VFV87_13980"
     CDS             2566..3897
                     /locus_tag="VFV87_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104923.1"
                     /translation="MSELSRRSFLNQSAGFVTAAALASAAKLGLAAAGDPVNVALIGP
                     GGMGSAHLNLLAVRKDLRVKYVCDPDANRAASAAKVVEQNGGSAKAVKDMREVFDDKS
                     IEAVWIATPDHWHCPAAILAVEAGKHVYVEKPVSHNIREGRLLLEAARKHKKVVQVGT
                     QSRSTPTVARAMEIVKSGEIGEVLVAKAWNSQLRGSIGHQQPSDPPAYLDYDLWLGPA
                     PQRPYQANLLPGIWRWWYEFGCGDIGNDGVHDIDVARWGLGVTGAPSTVAALGSKFFF
                     DDDQQFPDTQYCVFEWDAPTGERRASAPRGKRQLIFEQRDWSPYVQEGYENGAAFYGT
                     KGMLVMGHTSGFKVFGPRNKPGETGSGSVDLPAHHQNFLDCIRSGGRPNADIEEGHQS
                     ALLVHLANIACRVQRTLHFDPASEQITGDEEANQLVRRQYREGHWAVPKGV"
     gene            3909..4205
                     /locus_tag="VFV87_13985"
     CDS             3909..4205
                     /locus_tag="VFV87_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104924.1"
                     /translation="MPADYRQVDPAELRLPSSRAAGADPYKLQRQIAKHGTSIVGMPA
                     LLAYEGSDGVLELVDGVTRATRVAKLLPGTKVRVEVIGKLRRPRAGYRRIGDAL"
     gene            4220..4420
                     /locus_tag="VFV87_13990"
     CDS             4220..4420
                     /locus_tag="VFV87_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104925.1"
                     /translation="MTELLERLAELRGHCPELRFGQLLATIGMLAEDDTGHGLWEVED
                     AEFAAAVERFALDLARRGSVPT"
     gene            4497..4736
                     /locus_tag="VFV87_13995"
     CDS             4497..4736
                     /locus_tag="VFV87_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104926.1"
                     /translation="MNTITVEGSLGTALSGLKSQAILCDAHGRALGVYSPLAAQPQLE
                     DLQLEPPLSIAETEELRQNRTGKPLEEILQRLGAQ"
BASE COUNT          847 a         1605 c         1507 g          799 t
ORIGIN      
        1 gtctggtcca tttcagtcaa gcgaaaaaaa gatttcctga aatttcttgc cccccacacc
       61 aacaagtcgc tccagcgagg gatgccgacg acagaccgca cctttcctcg ccgcagagcg
      121 gcggccgatg gtagccgggc ctttcaaggc ccggaacact aaggacgatt gcctgccggc
      181 gtcgcggagc gacgctggaa caccccgtgc cacggttcgc cccgttcggc tcttgcgccc
      241 ctccgatcgc gcgtcgctac gcgacgcccg gacgagcttg tcgccttgcc ccgcgggtta
      301 aaacccacgg ctaccatcgc tccgtcgccc cgcgacgatc tccagccggc ttattctcgg
      361 gccgccagcg gctctcgtcg ctcgacaatc ggcgggcaga ccttggccat ctcggcattg
      421 agggcgatga attcgcgctg gtcggcggga acgtcgtctt cgtagaagat cgcttcgacc
      481 ggacactctg gcaagcacag cccgcagtcg atgcactcgt ccgggtcgat gtacaccatc
      541 tgctcgccct ctcggaacgc ctccaccggg caaaccacaa cgcaatcggt gtacttgcag
      601 ccaaagcagt tcctggtgac gacgaatgcc atgtcttcct cctatgctcg gatttgaccc
      661 tctacctggt ggatgcaaat cgccggcggc tggcggacaa aaaatcccat ccggaagtgc
      721 tgctacccgt atgacacggc gcggcataga cttgcgccca tgtcccgcga ggaagtcaac
      781 tcgctgtcgt cagccgacct gctgcggcga ttgcatgcgg gcgacgaagc ggctgccgaa
      841 gcggtcttcg agcgctatgc cgaccgcctc acacgcctgg cccaaagccg attgaccgtc
      901 aggctggcca gccgcatcga tcccgaagac atcgtgcaat cggcctatcg cagcttcttt
      961 gtggcggccc gcgcggggcg attccgcgtc cggagcggcg gcgatctgtg gcggctgctg
     1021 gtcgaggtca cgctgcacaa gctttaccgg caagcggccc atcacggcgc gcagcggcgc
     1081 tccgttgccc gcgaagtccg catcgacgac tccgcgtcat ccgccggagt gccgaccggc
     1141 ggcgagccga cgccagacga agcgctcgcc gctgcggagg aactggaagc gattttcgca
     1201 cagctctccg accgtgtccg gcaggcgctg gagctgcgcc tgcaaggtta cgagcatgaa
     1261 gagatcgccg agcggctggc gtgcagcgaa cggaccgtgc ggagatcgct ggccgaggcg
     1321 cggcgcgtca tggctgcccg cgccggcggc ggcttcgtgc cgagcgccgc ccggcggcgc
     1381 gccgtgccga caccttcgcc agcgatcccg gcagcaccga tcgaaatcgc atcgcccatc
     1441 ccctggagcg acctggtgct gcagcagcaa atcggcgcgg gggccactgg caaggtctat
     1501 cgggccttgc gccgcagcac cggtgcagaa gtcgccgtca agttcctccg caagtcgctc
     1561 cttcgcgagc cggccgtcat cgagcgattc cttcgcgaag caaaaactgt tgcccagatt
     1621 gctcagccgg ggatcgtcgg catgcacggc gcggggcgaa cacccagcgg tggttatttc
     1681 ctggtgatgg atctgattgg cggcggcgac ctggaccgcc tcaggcgatg ccagccggtc
     1741 tcgccaccag acgcggcggc gtgggtcgct caggctgccc ggatcgtgca ttccgcgcat
     1801 gagcagggcg tcgtgcactg tgacctgaag cccggcaacc ttctcctgga cgagcagggc
     1861 cagatacacg tcaccgattt tggactcgcc gcccgcgcgg ctgccggcga atcgaacctg
     1921 ctggccggta cgcccgcctt catggcgccg gagcaagtcg atcccgcctg ggggccgatc
     1981 tcgccgcgca ccgacatctt cggtctcggc gcggtgctgt tcttcctgct ctttggccaa
     2041 ccgccgcaca cgggagcgac catcgccgaa gtcctcagcc gcgtcgccgc cgactcgccg
     2101 gtagctatcc ccgatcaacg cgatgatcgt ctgaatcttg atctagtcgc catcatccgc
     2161 cgctgcctcg ccaaatcgcc cgccggccga ttccccaccg cagccgagct tgccgctgga
     2221 ttgaggacat gtggcgtcgc atgagtctga tcagaaggaa tcgaagatga cgaaggttgc
     2281 cggcggcgag gcgcggccgg tccaggcttc acgcgttacg ctgccgcgca agcgggacga
     2341 ttgcccgcag gttgatttcc gatacgccgc gcggtcgaat cctggctgcc cgcagggggt
     2401 cgggagtcaa atgcgacatt cgtattccat gatggttgac cgtgaccgca attgactccc
     2461 gaccccttcg cggggacccg ctacgccttc ggctacgcgt taaacgaact cctgatccct
     2521 gactcctgat ccctgactcc tgatccctgc ccccggagcc ccgccatgtc cgaactctcc
     2581 cgccgctcgt ttctcaatca gtctgccgga ttcgtcactg ccgccgcgct ggcttcggcg
     2641 gcgaagctgg gcctggcggc tgccggcgat cctgtgaacg tcgccctgat cggtcccggc
     2701 ggcatgggat ccgcccacct caatctgctc gccgtccgca aggacctccg cgtcaagtac
     2761 gtctgcgatc ccgacgccaa ccgcgccgct tccgccgcca aggttgtcga gcagaatggc
     2821 ggctcggcca aggccgtgaa agacatgcgg gaagttttcg acgacaagtc gatcgaagcg
     2881 gtctggatcg ccacgcccga ccactggcac tgtcccgccg cgattctggc cgtggaggcc
     2941 ggcaagcacg tctatgtcga gaagccggtc tcgcacaaca tccgcgaggg gcgcctcttg
     3001 ctcgaagcgg cccgcaagca caagaaggtc gtgcaggtcg gcacacaaag ccgcagcacg
     3061 ccgaccgtcg cccgggcgat ggagatcgtc aagagcggcg agatcggcga ggtgctcgtc
     3121 gccaaagcct ggaacagcca gctccgcggc agcatcggcc accagcagcc cagcgatccg
     3181 ccggcttatc tcgactacga cttgtggctc ggcccggcgc cccagcggcc gtatcaggcg
     3241 aacctgctgc cgggcatctg gcggtggtgg tacgaattcg gctgcggcga catcggcaac
     3301 gacggcgtgc acgacatcga cgtcgcccgc tggggcctgg gcgtgaccgg cgctccatcg
     3361 actgtcgcgg ccctcggcag caagttcttc ttcgacgacg atcagcagtt ccctgacacg
     3421 cagtactgtg tgtttgagtg ggacgcaccg accggcgagc ggcgggcgtc agccccccga
     3481 ggcaagcggc agctcatctt cgagcagcgc gactggtcgc cgtacgtgca ggaaggctat
     3541 gagaacgggg cggccttcta cggcaccaag ggcatgctcg tcatggggca caccagcggc
     3601 ttcaaagtct tcggcccgcg gaacaagccg ggcgagaccg gcagtggcag cgtcgatctg
     3661 ccggcccacc accagaactt cctcgactgc atccgcagcg gcggccggcc gaatgccgac
     3721 atcgaagagg ggcaccaatc ggccctgctc gtacacctgg cgaacattgc ctgccgcgtg
     3781 cagcgaacct tgcacttcga tccggcgtcc gagcagatca ccggtgacga agaagccaac
     3841 cagctcgtcc gccgccaata ccgcgaaggc cactgggcgg tgccgaaggg ggtgtgagtc
     3901 atcaaaccat gcccgctgac tatcgacagg tggatcccgc ggagttgcgg ttgccgtcgt
     3961 cccgggctgc cggtgctgac ccctacaaac tccagcgcca gattgccaaa catggcacat
     4021 ccatcgtcgg gatgccggcg ttattggcct acgagggttc tgacggcgtg ttagaattgg
     4081 tggacggcgt tacgcgcgcg acgcgagtcg cgaaattgct tcccggtacg aaagtgcgcg
     4141 tcgaggtgat cggcaagctg cgccggccga gagccggcta tcgccgaatt ggggatgcac
     4201 tatgaaaaca accgattccg tgacggaatt gctcgagcgt cttgccgaac tgcgcggcca
     4261 ctgtcccgag ctgcggttcg gacagcttct ggccaccatc ggaatgctgg ccgaggacga
     4321 tacaggccac gggctctggg aagtcgagga tgcagaattc gccgcggccg tcgaacgctt
     4381 tgcattggac ctggcaagac gcggttcggt gccaacgtga gagacgcggt gaatggcccg
     4441 gcgaggggga agccaaccag tagcttcatt cagaattcga attgtcggat cgtgacatga
     4501 ataccatcac cgtcgaagga tcactgggta cggcgctaag cggtctgaaa tctcaagcca
     4561 tcctgtgcga tgcgcacggg cgggcgctgg gggtgtactc gccgctggcg gcgcagccgc
     4621 aactcgagga tctgcagctc gaacctccgc tgtcgatcgc cgaaaccgaa gagctccggc
     4681 agaatcgcac gggcaagccg ctggaggaga ttctccaacg gcttggcgcg caatgagcta
     4741 tcccggcgcg gaatagcc
//
LOCUS       DASUGB010000730         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_635255, whole genome shotgun sequence.
ACCESSION   DASUGB010000730 DASUGB010000000
VERSION     DASUGB010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635255"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..>2384)
                     /locus_tag="VFV87_14000"
     CDS             complement(323..>2384)
                     /locus_tag="VFV87_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5104927.1"
                     /translation="TDVLEDADGSLLIVDTGGWYKLCCPSSQLVKADVLGAIYRVRRT
                     DSRAVADPRGLKIDWKDLSAESRADLLGDPRPAVRKQAIERLVAKGPVSAAPLSKILV
                     DRQSAPIAARAAISAACRIDSNLARRLIHEVMRHEDETVVQAAVHAASTYREGTAVAQ
                     FVRLLRSPSLHNRRAAAEGLGRIGRDESPLLIEALADPNNDRALEHSLTYALIELADR
                     DALQRNVNHANPRVRRCVLIAADQVGVDLQPQVVLAGLQDTDPAIRDTARWIAARHPE
                     WDAELSAFLRQRLRASDLTDTEQAALVDLLAKLAKGPAIQRLLSEVAATNDSAPASRA
                     LAAMAASDVRELPAAWASILPALLAVDSPQLSSSLALVRAIPPAKADAEKLAAALTQL
                     ANSDAAAELRLTAFAAMPAEHRPISPSQLKVLLSHVHRDQPANLRGLAADALSRAELT
                     PQQLQQLAAALPAAAPLELDRLLAPFAQSTDEAAGLALLASLRAPELRASVTVDAIRQ
                     RLAKYPRAVQEQAEQLYAELDADFAAQRSRLEKTLAALPAGDVRRGQAVFNSAKTSCR
                     ACHTIGYVGGKHGPDLTRIGQIRQPRDLLESILFPSASFVRSYEPLAVRMLDGQVYSG
                     VVKSDAPMELVLTVAADKEVRIVKDEIEIAQPGKVSIMPAGLDKQLSLQELADLVEFL
                     KNCR"
BASE COUNT          431 a          734 c          796 g          423 t
ORIGIN      
        1 ggaccggtac tgtgaattcg gctggggagc ctaccgcaag cactccgaca tctgggtgac
       61 caacccgtcg ttcacaatcg ccaaattcga ggaagcgctg acaaaagcag ccgcctgaaa
      121 caactctcct gcaaaggtcg cactttgaaa acctccgagc aactcaaacg ggaactgatt
      181 gggttccacg aggtggctca cgagtcggca aatgggcggg tgttttgaaa gaccaatggc
      241 tcgctgagcc taatccctgc aggctctgcc tggtcgtgac ggcgagcgcc gcggatgttt
      301 gcccctgctt tcgccgagtc ctttagcggc aattcttcag gaattcgacg agatcggcca
      361 attcctgcag cgagagctgc ttgtcgagtc cggcgggcat gatggagacc ttgccgggct
      421 gcgcgatctc aatttcgtcc ttcacgattc tcacttcctt gtcggccgcc acagtcagca
      481 cgagttccat tggagcatcg ctcttcacga cgccgctgta gacctggcca tccagcatgc
      541 ggaccgccag cggctcgtag ctgcgcacga agctggcgct gggaaagagg attgactcca
      601 gcagatcccg tggctgacgg atttgtccga tgcgcgtcag atcgggaccg tgcttgccgc
      661 cgacatagcc gatcgtgtgg caggcccggc aggaggtctt ggcgctgttg aagacggctt
      721 gcccgcggcg gacatcgccg gctggcaagg ccgctaatgt cttctccaag cgcgaacgct
      781 gtgcggcaaa atcggcgtcg agctcggcat agagttgctc cgcttgctcc tgaacggccc
      841 gcggatactt ggcgaggcgc tggcgaatgg catcgactgt tacgctggcc cgcagctccg
      901 gtgcgcgcaa cgaggccaac agcgccaagc cggccgcttc atcggtggac tgcgcgaatg
      961 gtgcgagtag tcgatcgagc tccagtggtg cagccgcggg gagcgccgcc gcaagctgct
     1021 gcaactgctg gggcgtaagc tcggcgcgcg agagggcatc ggcagccagg ccgcgcaggt
     1081 tggcgggctg gtcgcggtga acatgcgaca acaaaacttt cagttgggac ggcgagatag
     1141 ggcggtgttc tgcaggcatg gcagcaaacg ccgtcaatcg aagctcggcc gcggcgtctg
     1201 aattggcgag ttgcgtgagc gctgcggcca gcttttcagc gtcggccttg gccggcggaa
     1261 tggcccgcac aagggccagt gacgaggaaa gctgcggcga atcgacggcc aaaagtgcag
     1321 gcaggatcga cgcccaggca gccggcaact ctctcacgtc cgatgccgcc atagccgcca
     1381 aggccctgct cgcgggggct gaatcattcg tggccgccac ttcggacaat agtcgttgaa
     1441 tggcgggtcc ctttgccagc tttgccaaca ggtcaaccag cgcggcttgc tccgtgtcgg
     1501 tgagatcact ggcccgcagg cgctggcgca ggaatgcgga tagctcggca tcccattccg
     1561 gatgccgggc cgcaatccag cgggcggtat cgcggattgc gggatccgtg tcctgaagac
     1621 cggcgagcac gacctgaggc tggaggtcca cgccgacctg gtccgccgct atcagaacgc
     1681 agcggcggac gcggggattc gcatggttca cgttccgctg cagtgcgtcg cgatcggcca
     1741 gctcgatcaa ggcataggtc agcgaatgct caagcgcgcg atcgttgttg ggatcggcca
     1801 gcgcctcgat caagagtggc gactcgtcgc ggccgatgcg ccccaggccc tcggcagcgg
     1861 ctcggcggtt gtgcagcgaa ggcgacctga gcaggcgcac gaactgagcg acggccgtgc
     1921 cttcgcggta ggtgctggcc gcatgcacgg cggcttgcac gacggtttca tcctcgtgtc
     1981 gcatcacctc atgaatcaac cggcgggcca ggttgctgtc aatgcgacaa gcggcggata
     2041 ttgccgcgcg ggctgcgatg ggggcggact ggcggtcgac caggatcttc gacagcggcg
     2101 cagcgctgac gggacctttg gcgaccagcc gctcgatcgc ctgtttccgc acggctggcc
     2161 gcggatcgcc gagcaggtcg gcccgactct ccgccgagag atccttccag tcgatcttca
     2221 ggccgcgcgg atcggcgacc gctcgggagt cggtcctgcg gacgcggtag atcgcgccaa
     2281 gtacgtcggc cttcacgagt tgcgaactgg gacaacaaag cttgtaccac ccaccggtat
     2341 cgacaatgag caggctcccg tcggcatctt ccaggacgtc ggtt
//
LOCUS       DASUGB010000731         8330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_79744,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000731 DASUGB010000000
VERSION     DASUGB010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79744"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFV87_14005"
     CDS             complement(<1..214)
                     /locus_tag="VFV87_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5104928.1"
                     /translation="MTWIALRMLMGDRAKYFGLIFGVSFATLLMTQQVSIFMGILKRT
                     ASQILDIRDAEIWVMDNQVRFIDEVPA"
     gene            complement(211..897)
                     /locus_tag="VFV87_14010"
     CDS             complement(211..897)
                     /locus_tag="VFV87_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5104929.1"
                     /translation="MTSIDVRERRKAETREKILAAGRELLLSQGFHGFSLRKLAAKVH
                     YTATAIYLHFPDKDALLGELVDREFIRFRQIFDKTSQISDPIERLRKMGLVFVDFALA
                     QPDVYKILFLNTQVEAFPRIGLIERGNPAQDCYAYLRTTITDAIAAGRFRAELSDQDL
                     VAQVFMGGVHGIVSLHIARGKDPWVNWRPVEQRAQLLIDGLIRGLTIPTSHETHSTPS
                     APATFAEDLT"
     gene            complement(1113..1751)
                     /locus_tag="VFV87_14015"
     CDS             complement(1113..1751)
                     /locus_tag="VFV87_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104930.1"
                     /translation="MIRSLAAIVLIAIACSPASAQYYGGGWGGGGWGYHSSTLEEGIQ
                     RGYADVVRSQGMANLLNSQAAINLQDADKKYLENRLTATQTYFEMRRYNQEARRAERS
                     SPLSNEQYVRLARQQAPARLSVSQLDPFTGQINWPSPLRREKYDALRGQIETLFQAQA
                     EGTILADDEIGQKCDELLAALKDDIATFKQTDYIAAKNFVTSLAYEARFPRQ"
     gene            1884..3626
                     /locus_tag="VFV87_14020"
     CDS             1884..3626
                     /locus_tag="VFV87_14020"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5104931.1"
                     /translation="MRLLLISGLASFLAIFASSVCPGIEPTVAELAAQLKSQTPAEQH
                     AAADGLADLGPKAAEAVPQLVAALESKDAELRWRAARALGVIGSPMGVDAVRKHAADE
                     NADVRAQAIFALGRLAAADKPSLEAITSHLTDPDTDVRRASVRALQMIKADRSVVIPL
                     VVKVLDDADPAVVMPALHAIAEAGPQVVPALTEALSHKEARYWACLALAEIGPDAKDA
                     VPALVKVLDDERPEVRLQAIIALGEIGPAAKPAVSELTEELGDDSREIDIAAVFALGR
                     IGDPSASAAVAKLEKSDDKLMHTLATWTLAKLNSADKTRMKDAVDHLVGNLGGEDRNL
                     AHMSARAIVELQPDPEILRPAMEAAMEKADAATAERIISAYASLGPKVVPLAIKALND
                     GDAKRRMRALAVLGRLGPEAAPAVSDLARYLKSDDPKLRTEVLFVLAAIGPKAAEATA
                     PVTAALSDSDREVALTAGFCLGKIGPAAKSAAPELQKLLKSDDKMTALTGVWALLQIG
                     PQDPSLAKVAVPLLAEALSHEREYIRVEAAMALGKLGKAAEPAVPALEQAATADGSPA
                     VRQAAAEALKQIKK"
     gene            complement(3700..4668)
                     /locus_tag="VFV87_14025"
     CDS             complement(3700..4668)
                     /locus_tag="VFV87_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5104932.1"
                     /translation="MTRTLIALSLAVLLLAAPLAAQEKNKKAPPARPMPTAADFAYGK
                     DSERQKFDFWQAKSDSPTPVVLLIHGGGWMGGDKSSYGASAIQPYLDAGISVASINYR
                     FIPQAMEHKVEPPVKGPLGDAARALQTIRSKAKEWNLDPARVGATGGSAGACTSLWLA
                     LHDDLADPKSDDPIARQSTKLQCAAVVGAQTSLDPKELRAWMPNAIYGGHAFGFTAAG
                     RSRAQEFDLLIENREKVLPWIKEYSPIELVSKDDPPIYLDYPNQKTPPVIGQEGTDPT
                     HSAIYGIKLEEKMEEAGVECVLSYPGHQDEKYGSVASFLITKLKAK"
     gene            4775..6349
                     /gene="dacB"
                     /locus_tag="VFV87_14030"
     CDS             4775..6349
                     /gene="dacB"
                     /locus_tag="VFV87_14030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5104933.1"
                     /translation="MICTMFRRLPHVLFAAIGAIAFVSYSASLPAQDKLHERVDALLA
                     EPRFQHAHWGMLFVDLETGEVVLERNKDKLFAPASVTKLFSTATALDALGADHRFVTP
                     VYRRGMLKDDGVLDGDLILVASGDLTLGGRTTEKGEIDFKDDDHIYANWSGNGELTPQ
                     DPLAGLKDLARQVAAAGIRKVNDVYIDDRLFDHTEGSGSGPGTITPIVVNDNVLDFTF
                     EPGELDQPAKAEWRPQTALFAVDAQVQTVREDQPLEITIERESRKFVIRGKLPANKKN
                     VVRIVEVPDARAFARALFVEALEASGVEVAAEAADPSRDGKLPVTEEYSKLTKVAEFT
                     SPPFAEEARLILKVSHNLHASTLPLLVAVKNGKRSVADGLKLEGEFLARAGVDLDAVS
                     FGGGAGGSRADYVTPASTVQLLRYMASRPDFAVYEKALPILGVDGTLAKSVGADSPAK
                     GKVQAKTGTLVWDNLLVGRGLCTSKALGGYLTTASGKRLAFAAFVNGVPLKDGLTTRA
                     IGTDLGRLCEIVHAER"
     gene            complement(6352..6855)
                     /locus_tag="VFV87_14035"
     CDS             complement(6352..6855)
                     /locus_tag="VFV87_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104934.1"
                     /translation="MNWRRIQFVISIAILGCILLLLCPSFGSIHYFSPDTLQSRYQVE
                     KLFPGTRIPIYRSGYRESESQLVDYLIRKGHWKPIATKSPRWVLTSHSNSQWKDGQSY
                     VHKEFFWRDEKWVDWSEKNPKRAAVLWPQVLSILRDVGRNPNEACELMFDAEYETRTP
                     AESEFRQ"
     gene            6981..7538
                     /locus_tag="VFV87_14040"
     CDS             6981..7538
                     /locus_tag="VFV87_14040"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5104935.1"
                     /translation="MIDPVELLYERVLVVRCQAGDEAAFEELVERYSPRLRYFVLKLL
                     GTERRGGRSLHAEDLLQDVWLDAFRGLPRLADPQAFRAWIYRIARDRALGLLRKSKRT
                     EQPLGEIHVPEEAADEDEFSPEDAARIHAGLDELPAEQREVLVLRFLEDMSYDEIARV
                     TSQPLGTVRSRIHYGKRALRRLLET"
     gene            7643..8119
                     /locus_tag="VFV87_14045"
     CDS             7643..8119
                     /locus_tag="VFV87_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104936.1"
                     /translation="MSENDLGRALLSLDPDKVADASDVRQQTRRILDQDRRRVWWLTA
                     LTVFLWATALLMVIGMLIAFGLVFPWQAKLRDPARIAQLPPQVLQEAQSQAQIAFQMV
                     TVGVTCSVGILAMAALATILLVLATRRATLRQINASLLEISQQLKELRDSPAPTRK"
BASE COUNT         1544 a         2556 c         2677 g         1553 t
ORIGIN      
        1 gggccggcac ttcgtcgatg aagcggacct ggttgtccat gacccagatt tcggcgtcgc
       61 ggatgtcgag aatctggctg gccgtccgtt tgaggatgcc catgaagatc gagacctgct
      121 gcgtcatgag cagcgtggcg aacgagacgc cgaagatcag gccgaagtac ttggcccggt
      181 ctcccatcaa catgcggagg gcgatccagg tcatgtcagg tcctccgcaa aggtagcggg
      241 cgcactgggg gtgctgtgcg tttcgtggga ggtgggaatt gtcagcccgc gaatcaaccc
      301 gtcgatcaag agctgggccc gctgctcgac gggccgccag ttgacccagg gatctttgcc
      361 gcgggcgatg tgcaacgaga cgatgccatg cactccgccc ataaagacct gagccaccag
      421 atcctggtcg ctcagttcgg cgcggaagcg gccggccgcg atcgcgtcgg taatcgtcgt
      481 ccgcaggtag gcgtagcagt cctgagccgg attgccccgc tcgatcaggc cgatgcgcgg
      541 gaaggcttcg acctgcgtgt tgaggaacag gattttgtac acatccggct gggcgagggc
      601 gaaatcgacg aacaccagtc ccatcttccg cagccgctcg attggatcgg agatttgact
      661 cgtcttgtcg aagatctggc gaaagcggat gaattcgcga tcgaccagct cgccgagcag
      721 ggcgtctttg tctggaaaat gcaagtagat tgcggtcgcc gtgtaatgga ccttggcggc
      781 cagtttccgc agcgagaagc cgtgaaagcc ttgagacagg agcagttctc gccccgccgc
      841 gaggatcttc tcccgagtct ccgctttgcg tcgttctcgc acgtcgatcg acgtcatggt
      901 atttccttta catgtcacct aacgacgtta ttttactgaa cagtgttaag tattcaaggc
      961 cgatcgacgt gccgccgaaa aaatattgac cgcaagtcat tcagtcgaaa tgacttacga
     1021 aagatagaat gctgccagat gcgttctgct agacagttac atcgggtgcc taactcaagc
     1081 aaagacgtcc caggaccgag gcgatgcgga cctcattgcc ggggaaagcg ggcctcataa
     1141 gccaggctgg tcacgaagtt cttggcagca atgtagtcgg tctgtttgaa ggtggcgatg
     1201 tcgtccttga gtgcggccaa cagttcatcg cacttttgcc cgatttcgtc gtcggccagg
     1261 atcgttccct cggcctgggc ttgaaagagc gtctcgattt gtccccgcaa ggcgtcgtac
     1321 ttttcgcggc ggagcgggct gggccagttg atttgaccgg tgaagggatc gagttggctg
     1381 acgctcaggc gggccggcgc ctgctgccgg gccagccgca cgtattgctc gttggaaagc
     1441 ggcgaggagc gctcggcccg gcgggcctcc tggttgtagc gccgcatctc gaaataggtc
     1501 tgcgtcgctg tgaggcggtt ttccaggtac ttcttgtccg catcctgaag gttaatggcg
     1561 gcctggctat tcagcaaatt agccatgccc tgcgagcgga cgacgtcggc atagccccgc
     1621 tgaatgccct cttccagcgt ggaagagtga taaccccaac cgccaccgcc ccagccgccc
     1681 ccataatact gggcactggc cggcgagcag gcgatcgcaa tcaagacgat ggcggcgagc
     1741 gagcggatca tgggcggcac tccaggtatt tcactcaaat ccaagttgca ggcgcaggac
     1801 cgtagaatgt ggatttaagc atactataat ggcgacaagc tcctcgcaac tgtcggcgct
     1861 cgttctcaac tgatggtgca tccatgcgac tgcttctgat ttccggactg gcaagtttcc
     1921 tggcgatctt tgcatcgtcc gtctgccctg gcattgaacc gacggtggcc gagttggccg
     1981 cacagctcaa atcgcagacg ccggccgagc agcacgccgc cgcggacggg ctggcggacc
     2041 tgggtcccaa agcggctgag gccgtgcccc agcttgtcgc tgcgctggaa tcgaaagacg
     2101 cggagttgcg gtggcgcgcg gcccgcgcgc tgggtgtgat cggaagccct atgggagttg
     2161 atgccgttcg caagcacgca gccgacgaga acgccgacgt gcgagcccag gcgatctttg
     2221 ccctggggcg cctggcggca gcggacaagc catcgctgga ggcaattacg tcgcacctga
     2281 ctgaccccga taccgatgtg cggcgggcct ccgtccgcgc cctgcagatg atcaaggcgg
     2341 accgcagcgt cgtgattccg ctggtggtca aggtactgga cgatgccgat cccgctgttg
     2401 tcatgccagc cctgcacgcc atcgccgaag ccggtccgca agtcgttccc gccctgacgg
     2461 aagccctctc gcacaaagaa gcccgctact gggcgtgtct ggccctggcc gagatcggac
     2521 cggacgccaa ggacgcggtt ccggctctgg tcaaggtttt agatgacgag cggccggaag
     2581 ttcgcctgca agccatcatc gccctgggcg agattggccc tgccgcgaaa ccggccgttt
     2641 ccgagttgac cgaggagctt ggtgacgact cgcgggagat cgacattgcc gcggttttcg
     2701 ccctcggccg gattggagac ccctcggcat cggcggcagt ggcgaaactg gaaaagtccg
     2761 acgataagtt gatgcacacg ctggccacct ggaccctggc gaagttgaac tccgcagaca
     2821 agacgcgcat gaaggatgcc gtcgatcatc tggtcggcaa tctgggtggt gaggatcgca
     2881 acctggccca catgtcggcc cgggcaatcg tcgagctcca gcccgatccg gagatcctcc
     2941 gccccgccat ggaggcggca atggaaaaag ccgatgccgc cacggccgag cggatcatct
     3001 cggcgtatgc ctcgctgggg ccgaaggtcg tacccctggc gatcaaggct ctgaacgacg
     3061 gcgacgccaa acggcggatg cgggcgctgg cggtgctcgg ccgcctgggg ccagaggcgg
     3121 ctccggcagt gtccgatctg gcgcgctatc tcaaaagcga cgatccgaaa ctgcggaccg
     3181 aggtgctgtt cgttctcgcc gcgatcgggc cgaaggcggc tgaagcgacg gcgccggtta
     3241 ccgcagccct gagcgattcc gaccgcgaag tggccctgac ggctgggttc tgcctgggca
     3301 agatcgggcc ggcggcgaag tctgctgcgc ccgagctgca aaaactcctc aaatcggacg
     3361 acaagatgac ggcgctgacg ggggtctggg cgctgttgca gattggcccg caggatccat
     3421 cgttggccaa agtcgccgtg ccgctcttgg ccgaggcgct gagccacgag cgggagtata
     3481 ttcgcgtcga agcagcgatg gccctgggca agctgggcaa agcggccgag ccagccgtgc
     3541 ccgccctgga acaagctgct actgccgacg gcagcccggc cgttcgccag gccgccgccg
     3601 aagcgctcaa gcagatcaag aaataagccg agcgcaggac aggtctttaa cctgaccgcg
     3661 cgggacgggt tggaaacccg tcctacgcgg ccacgaagct tacttggcct tcagcttggt
     3721 gatcaggaag ctcgcgaccg aaccgtactt ctcatcctgg tggccgggat acgacagaac
     3781 gcactcgacg cccgcttcct ccatcttctc ctccagcttg atgccgtaga tggccgaatg
     3841 cgtcggatcg gtcccttcct ggccgatgac cggcggcgtc ttctggttgg gatagtcgag
     3901 gtagatcggc gggtcgtcct tgctgaccag ctcgatcggc gagtattcct tgatccacgg
     3961 cagcaccttc tcgcgattct cgatcaacag gtcgaattcc tgcgctcggc tgcggccggc
     4021 agctgtgaag ccgaaggcgt gcccgccgta gatcgcgttg ggcatccacg cgcgcagctc
     4081 cttgggatcg agcgaagtct gggcgccgac gaccgccgcg cactgcagct ttgtcgattg
     4141 gcgggcgatc ggatcgtcgc tcttgggatc ggccaggtcg tcgtgcaggg ccagccacag
     4201 cgaggtgcag gcccccgccg agccgccggt cgcgccgacg cgcgccggat cgaggttcca
     4261 ctccttggcc ttggagcgga tggtttgcag ggcccgcgct gcgtcgccca gcggtccctt
     4321 caccggcggc tcgaccttgt gctccatcgc ctgcgggatg aagcggtagt tgatcgacgc
     4381 gactgaaatg ccggcatcga ggtacggctg aatggcggag gccccatagc tgctcttgtc
     4441 gccccccatc cagccgccgc catgaatcag cagcacaacg ggcgtcggcg aatcggactt
     4501 ggcctgccaa aagtcaaact tctgacgctc ggaatccttg ccgtaggcga aatccgccgc
     4561 ggtcggcatc ggccgcgccg gcggtgcctt tttgttcttt tcctgcgcgg cgagtggcgc
     4621 ggcaagcagc agcaccgcga gtgagagagc aatcagtgtg cgagtcatgc aagtctcctt
     4681 tttgcgagtg gatcggaccg ctcgatccta caacctgaga agttgccaag ctagaatgtg
     4741 gcgacggaca cgcgcccttt ctcactccac cgccatgatc tgcacgatgt ttcgccggtt
     4801 gccgcatgtg ctctttgctg ctatcggagc gatcgctttc gtttcctatt ctgcatcgct
     4861 gcctgcccag gacaagctcc acgagcgggt cgatgccttg ctcgcggagc cgcggttcca
     4921 gcatgcccac tgggggatgc tgttcgtcga tctggaaacg ggcgaggtcg tgctggagcg
     4981 gaacaaggac aagctgttcg cgccggcttc ggtgacgaag ctgttttcga cggcgacggc
     5041 gctcgatgcc ctgggcgcgg atcaccggtt cgtcacgccc gtttatcggc gggggatgct
     5101 gaaggacgac ggcgtgctcg acggcgattt gatccttgtg gcgagcggcg atctgacgct
     5161 tggcgggcgg acgaccgaga agggcgagat cgacttcaag gacgacgacc acatttacgc
     5221 caactggagc ggcaacggcg agctgacgcc gcaggacccg ctggccggcc tcaaggacct
     5281 ggctcggcag gtggcagcgg ccggcatccg caaggtgaac gacgtctata tcgacgaccg
     5341 gctgttcgac cacaccgaag gcagcggcag cgggccggga acgatcacgc cgatcgtcgt
     5401 caacgacaac gtgctcgact tcacgttcga gccgggcgaa ctcgatcagc cggcgaaggc
     5461 cgagtggcgt ccgcagacgg cgctgttcgc ggtcgatgcc caggtgcaga ccgtccggga
     5521 ggatcaaccg ctggaaatta cgatcgagcg cgagtcgcgc aagttcgtca tccgcggcaa
     5581 gctgccggca aacaagaaga acgtggtgcg gattgtggaa gtgcccgacg cgagggcttt
     5641 tgcccgggcg ctatttgtcg aggcgctgga ggccagcggc gtggaagtcg ccgccgaagc
     5701 ggccgacccg agccgcgatg gcaagctgcc ggtgaccgag gagtactcga aactgacgaa
     5761 ggtcgctgaa ttcacatcgc cgccgttcgc cgaagaggcc aggctgattc tcaaggtcag
     5821 ccacaacctg cacgccagca cgctgcccct tttggtcgcg gtgaagaacg gcaagcggtc
     5881 ggtggcggac ggtctgaagc tggaggggga attcctcgct cgggccggcg ttgatctgga
     5941 cgcggtcagc ttcggcggcg gcgccggcgg atcgcgggcc gactacgtca caccggcttc
     6001 gaccgtgcag cttctgcgct atatggcgtc gcggcccgac ttcgcggtct atgagaaggc
     6061 cctgccgatc ctgggcgtcg atggcacgct ggccaagagc gtgggcgcgg acagccccgc
     6121 caagggcaag gtgcaggcca aaaccggcac gctcgtctgg gacaacctgc tcgtcgggcg
     6181 cggcctctgc accagcaagg ccctcggcgg ctatctgacc accgccagcg gcaagcggct
     6241 ggcattcgcc gcgttcgtca acggcgtgcc gctcaaagac ggcctcacca cccgcgcgat
     6301 cggcaccgac ctcggccggc tgtgcgagat cgtgcatgcg gagcgctgag gctattgcct
     6361 gaactcgctt tccgccggcg tgcgggtttc gtattcggcg tcgaacatca actcacaggc
     6421 ctcattgggg tttcgaccga catcacgcaa gatggacagt acttgtggcc aaaggactgc
     6481 ggcgcgcttc gggttctttt cggaccaatc gacccatttc tcgtcgcgcc agaagaattc
     6541 cttgtgaaca taagattgcc cgtccttcca ctgcgaattg gaatggctgg tcagtaccca
     6601 ccgcggcgac ttcgtggcaa tgggtttcca gtgacccttg cggatcagat agtcaacgag
     6661 ttgtgactcc gactcccgat atccagagcg gtagattgga attcgcgtcc ccggaaaaag
     6721 cttctccact tggtaccgtg actgcagtgt gtcaggcgaa aagtagtgga tcgagccaaa
     6781 agatggacaa agtaacaata agatgcaccc gaggatcgcg atggaaatga cgaattggat
     6841 ccgtcgccag ttcatggttt ggcaccgttt actcgaccat gcactattat aaatgatcct
     6901 cgcgcgattc attgaacttt tccgctcccg ccgcaacctc tttcttggcg agcgaaatcc
     6961 tggactgaat tgaaagccgc atgatcgatc ccgttgagct gctttatgag cgggtgctgg
     7021 tcgtgcgctg tcaggctggc gatgaagccg ccttcgagga actggtcgag cggtattcgc
     7081 cgcggctgcg gtacttcgtg ctgaagctcc tgggcacgga acgccgcgga gggcgttccc
     7141 tacatgccga ggatttgctg caggacgtgt ggctggatgc gttccgcggg ctaccccggc
     7201 tggccgatcc gcaggcgttt cgggcctgga tttaccggat cgcccgcgac cgggcgctgg
     7261 gcctgttgcg aaagagcaaa cggacggagc agccgctggg cgagatccac gtgccggagg
     7321 aagccgcgga tgaagacgag ttttcgcccg aggacgccgc ccgcatccac gccgggctcg
     7381 atgaactgcc ggccgagcag cgcgaggtgc tcgtcctgcg gttcctcgaa gacatgagtt
     7441 acgacgagat cgcccgcgtc accagccagc cgctagggac cgtgcggtcg cgaatccatt
     7501 acggcaagcg cgccctgcgg cgcctgctcg aaacgtaggg tgggacaagg ccgccgcggc
     7561 cgcaggccca ccacgaattg acaacatcct ggtgggcctg cgcgagcttc gctcgctggt
     7621 cccaccttac aaggaaactc ccatgtccga aaatgacctt ggccgggcgc tactttcgct
     7681 cgatccagac aaagtcgccg acgcttccga cgtccgccag cagacgcgga gaattctcga
     7741 ccaggaccgc cgccgcgtct ggtggttgac ggccctcacg gtcttcctct gggcaaccgc
     7801 tcttctgatg gtcatcggaa tgctgatcgc cttcggcctg gtatttccct ggcaagccaa
     7861 gctgcgggat cccgcgcgaa tcgcccaact gccgccgcaa gtgcttcagg aagcgcaatc
     7921 ccaagctcaa atcgcattcc aaatggtcac cgtcggcgtc acctgctccg tcggcatcct
     7981 ggcgatggcc gcgctggcga cgatcctgct cgtcctcgcc acgcgccgcg ccacgctccg
     8041 ccagatcaac gccagcctgc tagagatttc gcagcagctt aaggagctgc gcgatagccc
     8101 agctcctaca cggaagtagc ccgaatcccc atgccgggct tcagaacgtc agttgccatt
     8161 gcctttccga tcgcgcactg attcaattca tgcgatagcc tttctccata tggctttgga
     8221 tggaggacaa gccaatgctg cgtcaccgga ctaacgaggc tggaatcgtt cttttgacgc
     8281 tattaaggtt ggcgacaacg gcggacggag aggacctgca gagtggcgtg
//
LOCUS       DASUGB010000732         5380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_775837, whole genome shotgun sequence.
ACCESSION   DASUGB010000732 DASUGB010000000
VERSION     DASUGB010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775837"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VFV87_14050"
     CDS             <1..1718
                     /locus_tag="VFV87_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5104937.1"
                     /translation="RQGSGKLLGEILIERGICTDDQVMECLAVVYGVPYAKLDPRISD
                     PKAVEVLPREYIEKNLVFPLFKIREALTVAVTEPTNLFLLDELHAITNLEIQVVATSA
                     KDVRRMITTLPDSKTFVIDDIIDDNAQTEVTLIETAIEDIGDATEFAGQSPVIRLVNY
                     IIYNAVKEGASDIHIEPAERAMRVRYRIDGRLYKSLEVPLNLLPAVASRIKIMASLDI
                     SERRLPQDGRVHVLLEGRKIDLRISTFPGSRGEKTVIRVLDTRSVSLNLRDLGFAEDV
                     LTPLQAAIQSPNGIVLVTGPTGSGKSTTLYAALNEIASMENNVCTVEDPIEYHLSMIN
                     QFQVQERVGLTFSKALRTLLRQDPDVIMVGEIRDDETARTAIQAALTGHLVFSTLHTN
                     DAVSAITRLVNMGVEPYLIGAALNAVLAQRLVRRICAKCREPYEPARPIRKAVERMGL
                     VVDKFYKGVGCRKCRNTGYSGRLGIHELLLINDELRDAIVAAASITELRRIADGEGMV
                     TLKHDGFRKVREGLTSLEEVIQICGDISTSAAAAGNERKTSSLAALQLAQAAAAKEQH
                     EGDDS"
     gene            1715..2914
                     /locus_tag="VFV87_14055"
     CDS             1715..2914
                     /locus_tag="VFV87_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5104938.1"
                     /translation="MTTFAYQARDALGHATSGTLEVESRDDALSRLRRDGLTVVELEE
                     DESHVSLIPRRVTATEIIYATSQLAVMVDTGITLSVALDSIAEHEKNPTLKAVLLDLR
                     ARVEGGEDFSTALARYPRYFDKTFVALIRSSEQTGTLAEMLETVSGYLRSQLEIRQKI
                     RASMAYPCVMAVLAVGVTIFLLTYILPKFEPLFSRKGVKLPRPTIIMMTASELLLDYW
                     YLWLALALALFFTWLFGRKTEPGRKILDWLKINIPVIGPMSRKVTLSRSIRTLGTMVA
                     SGVSMLDAIRLTAEVASNWYYERAWLRVLDQITEGNRICDALKPEEALFPATLVRMIG
                     AGEETGKLDKVLMKVSQFYDKEVEASLKATTSLIEPLMICAMGFVVGGIALGLLMPIF
                     SLSRPTG"
     gene            complement(3066..4214)
                     /locus_tag="VFV87_14060"
     CDS             complement(3066..4214)
                     /locus_tag="VFV87_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104939.1"
                     /translation="MARWILAIALVASSHAGPICPVSACPGCNATGATFCEEIAAMDI
                     AAIVRLAKSPPVKAGDVELPKATFEIVQILKGERLVRKGERFDALFFGEAKVGTAFLV
                     MGVGPPKIQWSTPLVLSDRGSEYLREILKLPKDGPERLAFVQSYLEDADEKLASDAYN
                     EFARAPHTDVKALKDRMDHDRLVRWINDPEIPAIRRRLYLLMLGVCGDEGDLPMLEGF
                     LRSKDRQAKAGLDMVISCYLTLKGESGLALVEELFLANTQADYADTYSAIMAIRLQAD
                     NFGGIGKPALIKALHPMLQRPELADLVIPDLARWEDWGAIDRLFVLYRDADPKNSWLR
                     VPVINYLRACPLAKAKDLLRECERIDPAAVKRANTFFPVPPSVGNTAG"
     gene            complement(4282..>5380)
                     /locus_tag="VFV87_14065"
     CDS             complement(4282..>5380)
                     /locus_tag="VFV87_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5104940.1"
                     /translation="AEVADGGTVDFGTTTVGTPRGRVITITNTGTGTLSLTPINANSL
                     PAGFLLVSNIGSTSLAPGQSTSFTVRMTASAAGTFSGSISIGNNDSDESPYDLVLTGV
                     VSGSQPQSQSEVRYIDNGSSGYSTAGTWYSSSSGRDGDANWTVNGSGSNVATWTFTGL
                     AAGQYRVSATWPSASHYASDAPYSVYNGSQFLGTSRVSQKAAPSGLSDAGSQWKDLGN
                     FTITGNTLVVKLSNDANGWVAADAIRIELIAAGSKYGHGALPGASPAQQLLASAALAE
                     GQSTGLLQQATDAVFSTDPEDAWLSVQETALSRIGAQLASQLSSEQPELEILHQTEEL
                     LDELAASLSQSREDSLGGLGARLAAILDQDA"
BASE COUNT          988 a         1708 c         1742 g          942 t
ORIGIN      
        1 agcggcaggg gagcggcaag ctgctgggcg aaatcctgat cgagcgcggc atttgcaccg
       61 acgaccaggt catggagtgc ctggcggttg tctatggtgt gccgtatgcc aagctcgacc
      121 cgcggatttc cgatccgaaa gcggtcgaag tgctgccgcg ggaatatatc gagaagaatc
      181 tcgtcttccc gctgttcaag attcgcgagg cgctgaccgt cgcggtcacc gaacccacga
      241 atctgttttt gctcgacgag ctgcacgcga tcacgaacct ggagattcag gtcgtcgcca
      301 cttccgccaa agacgtgcgg cggatgatca cgacgctgcc cgacagcaaa acgttcgtca
      361 tcgacgacat catcgacgac aacgcccaga ccgaagtcac gctgatcgag acagcgatcg
      421 aggatatcgg cgacgcgacc gaatttgccg gacagtcgcc cgtcatccgc ctggtcaact
      481 acatcatcta caacgccgtc aaggaagggg ccagcgacat tcacatcgag cccgccgagc
      541 gcgcgatgcg cgtccgctac cggatcgacg gacggctgta taagtcgctg gaggtgccgc
      601 tcaatctact gccggccgtc gccagccgca tcaagatcat ggcctcgctc gacatcagcg
      661 agcggcggct gccgcaagac ggccgcgtgc atgtcctgct cgaaggccgc aagatcgacc
      721 tccgcatcag caccttcccc ggcagccgcg gcgagaagac ggtcatccgc gtgctcgata
      781 cgcggagcgt ctcgctcaac ctgcgggacc tcggattcgc cgaggacgtg ctcacgccgc
      841 tgcaagccgc gatccaatcg cccaacggca tcgtcctggt caccggcccg acggggagcg
      901 gtaaatcgac gaccctctat gcggcgctga acgaaatcgc ctcgatggag aacaacgtct
      961 gcaccgtcga ggacccgatc gaatatcacc tctcgatgat caaccagttc caggtgcagg
     1021 agcgcgtggg gctgacgttc agcaaggccc tgcggacgct tttgcggcaa gacccggacg
     1081 tgatcatggt gggcgaaatc cgcgacgacg aaacggcccg cacggccatt caggccgccc
     1141 tcaccggcca cctcgtcttc agcacgctgc acaccaacga cgccgtcagc gcgatcacgc
     1201 ggctggttaa catgggcgtc gaaccgtatc tgatcggggc ggctttgaac gcggtgctcg
     1261 cccagcggct ggtgcggcgg atctgcgcca agtgccgcga gccgtatgag cccgcgcggc
     1321 caatccgcaa agcggtcgaa cggatggggc tcgtcgtcga taagttctac aaaggcgttg
     1381 gctgccgcaa gtgccgcaac acaggctata gcggacggct gggcatccac gaactcttgc
     1441 tgattaacga cgagctgcgc gacgcgatcg tcgcggcagc cagcatcacc gagctgcggc
     1501 ggatcgccga cggcgaaggg atggtcaccc tcaagcacga cggcttccgc aaggtgcgcg
     1561 aggggctgac gagcctggaa gaagtgatcc agatttgcgg cgacatcagc acgagtgcgg
     1621 ctgccgccgg caacgagcgc aaaacctcct ccttggccgc gttacagctt gctcaagcgg
     1681 ctgccgccaa agagcagcac gagggtgacg attcatgacg acctttgcct accaggcccg
     1741 cgacgcgctg ggccacgcga ccagcggcac gctcgaagtc gaatcgcgcg acgatgcgct
     1801 ctcgcgcctg aggcgcgacg gcctgacggt cgtcgagctg gaagaagacg agtcgcacgt
     1861 cagcctgatt ccgcgccgcg tcacggcaac ggaaatcatc tatgccacga gccaactggc
     1921 ggtgatggtc gatacgggga tcacgctctc ggtggcgctc gatagcattg ctgagcacga
     1981 aaaaaacccg acgctcaagg cggtgctgct cgacctgcgg gcgcgcgtgg aagggggcga
     2041 ggacttttcg accgccctgg cccgctatcc gcggtacttc gacaagacgt ttgtggccct
     2101 gatccgctcc agcgaacaga ccggcacgct ggccgaaatg ctggagacgg tgtcgggcta
     2161 tctgcgcagc caactcgaaa tccggcagaa aattcgggcg tcgatggctt atccctgcgt
     2221 gatggccgtg ctggccgtcg gcgtcacgat cttcctgctg acgtatatcc tgccgaagtt
     2281 tgagccgctc ttctcgcgca aaggcgtgaa gctgccccgg ccgacgatca tcatgatgac
     2341 cgcctcggaa ctgctgctcg attattggta tctctggctg gcgctggcgc tggcgctgtt
     2401 tttcacctgg ctcttcgggc ggaaaacgga gccggggcga aagattctcg actggctgaa
     2461 gatcaacatc ccggtgatcg ggccgatgtc ccgcaaggtg accctcagcc ggagcatccg
     2521 cacgctgggg acgatggtgg ccagcggcgt gtcgatgctc gacgcgatcc gcctcacggc
     2581 cgaagtcgcc tcgaactggt attacgagcg ggcctggctg cgggtgctcg accagatcac
     2641 ggagggcaac cggatctgcg acgccttgaa gcccgaggag gccctgttcc ccgcgacact
     2701 ggtgcggatg atcggcgcgg gcgaagagac cggcaagctc gacaaggtgc taatgaaggt
     2761 cagccagttc tacgacaaag aggtggaggc atcgctgaaa gccaccacca gcctcatcga
     2821 gccgctgatg atctgcgcca tgggcttcgt cgtcggcggt atcgccctgg gcctcctcat
     2881 gcctatcttc tccctcagcc gcccgacggg gtagcgcggt ggagcggccc gatcggggaa
     2941 attattcgcc gtcccagctc gcgaggcgcc ctcctcctgg ggcaacccgt ccatgacgtc
     3001 acgaactcgc ttcccggtca gaaggctgct acagcggatc caatacgcct gttctccgct
     3061 ccgcatcacc cagccgtgtt gcccacgctg ggcggcacgg gaaagaacgt gttggcccgc
     3121 ttcacggcgg cagggtcgat ccgctcgcac tcgcggagca ggtccttggc cttggccagc
     3181 gggcaggcgc gcaggtagtt gatcacgggc acgcgcagcc aactgttttt cgggtcagcg
     3241 tcgcgataga gcacgaacag ccggtcgatt gccccccagt cttcccagcg ggccaggtcg
     3301 gggatgacga ggtccgcaag ttccggtcgc tggagcatcg ggtgcagggc cttgatcaag
     3361 gccggcttgc caatgccgcc aaaattgtcg gcttgcaggc gaatcgccat gatggccgag
     3421 taggtgtcgg catagtcggc ctgcgtgttg gcaaggaaca gttcttcgac gagcgcgagt
     3481 ccggattcgc ctttgagcgt gagatagcac gaaatgacca tgtccaggcc cgccttggcc
     3541 tggcggtcct tcgatcgcag gaagccctcg agcatcggca gatctccctc atcgccgcag
     3601 acgccgagca tcagcagata caagcggcgg cgaattgcgg gaatctccgg gtcgttgatc
     3661 caccgcacca gccggtcgtg atccatcctg tctttcaggg ccttcacgtc ggtatgcggc
     3721 gcgcgggcga actcgttgta agcatcgctt gccagctttt cgtccgcgtc ttccaggtag
     3781 ctttgaacaa acgccagccg ctccgggccg tctttaggca acttcaggat ttcgcggagg
     3841 tattcgctgc cgcgatccga gaggaccagc ggcgtggacc actgaatctt gggcgggccg
     3901 acgcccatca ccagaaacgc agtgccgacc ttggcttcgc caaagaagag cgcgtcgaac
     3961 cgctcgccct tgcggacgag gcgctcccct ttcagaatct gcacgatttc gaacgtcgcc
     4021 ttcggcagct cgacgtcgcc ggctttgacc ggaggcgatt tcgccagccg cacgatcgcc
     4081 gcgatatcca tcgcggcgat ttcttcgcag aaggtggccc cggtggcgtt acagccggga
     4141 caggccgaga ccggacagat cggcccggca tgcgacgagg caaccagagc gatggcgaga
     4201 atccagcgag ccatgatgct gctcctgcaa agagcggacg gccgggttcc gctcgattgt
     4261 aggagcttgc gcccgccagt gctatgcatc ctggtcgagg attgccgcga gcctggcgcc
     4321 aaggccaccg agcgaatctt ctcgtgattg gcttaagctg gcagccaact catcgagcag
     4381 ctcttcggtc tggtgcagaa tctcgagttc aggctgctcg ctggagagct ggcttgccaa
     4441 ctgggccccg atgcgcgaca gtgcggtctc ttggaccgac agccaggcat cttccgggtc
     4501 cgtgctgaag acagcatccg tcgcttgctg cagcagtccg gtgctctggc cctcggccag
     4561 cgctgcgctc gcgaggagtt gctgggccgg ggaagcgccc ggtaaggccc cgtggccata
     4621 cttgctgccg gcggcaatca gctcgatgcg gatggcgtcg gccgccaccc agccgttggc
     4681 gtcgttggac aacttcacca ccagcgtatt gccggtgatc gtaaagttcc ccaagtcctt
     4741 ccactgcgag ccggcgtcgg acaggccgct gggagcagcc ttctggctga cccggctggt
     4801 gcccaggaac tgcgagccgt tatagaccga gtagggcgcg tcgctggcgt agtggctggc
     4861 gctcggccag gtggccgaga cgcggtactg gccggcggcc agtcccgtga acgtccacgt
     4921 ggcgacattg ctgccgctgc cgttgaccgt ccagttcgcg tccccatcgc ggccgctgct
     4981 cgacgagtac caggttcccg ccgtcgagta gccggaagag ccgttgtcga tgtaccgcac
     5041 ctcggactgg gactgcggct gcgagccgct gacgacgccg gtgagaacca gatcgtaggg
     5101 cgactcatcg ctgtcgttgt tgccgatgct gatggagccg ctgaacgtgc cggcggctga
     5161 ggcggtcatt cgcacggtga aggaggtcga ttgaccgggc gccaggctgg tcgagccgat
     5221 attcgagacc agcaggaagc ccgcagggag cgagttggcg ttgatgggcg tgaggctcag
     5281 cgtgccggta ccggtgttgg tgatcgtgat gacgcggcct cggggagtgc cgacggttgt
     5341 ggttccgaag tcgaccgtac cgccatcggc cacttccgcg
//
LOCUS       DASUGB010000733         7694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_80080,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000733 DASUGB010000000
VERSION     DASUGB010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80080"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..1948
                     /locus_tag="VFV87_14070"
     CDS             545..1948
                     /locus_tag="VFV87_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104941.1"
                     /translation="MSGNPMTGRSSRRSFLEAAGGLLAVAGATQLTTAGSQQPVRTAV
                     IGTGARGSDLLRALTTIQGCEVVAIADDYAPHLERGAKYAGPQAKTYSDYRKLLAEAK
                     PQAVVIAVPLYLHYQVASDAVAAGCDVFLEKTMCYSVAECRKLAAEVEQAKCVFQVGL
                     QRRANPIYQQAVAMVKAGVIGQVSAIKSQWHRNNNWRRPVPVPKGDAKYGELEHRLNW
                     RLYQRYSQGLMAELGSHQMDVANWVLGNTPQRVIASGGIDYWQDGREVHDNVFCIYDY
                     ELPQRETAPAGAGASAARGAGFQPAKNTAGEPSGNGESSAAPGLPRSGGPRYTVRVTY
                     SSLCNNAYEGAAELILGTKGSLFLTTTKGLFYREQLPDDPAWLKPGDAGNAAVLTAGK
                     TLKMSNSPWAHRGEPLEIDLLESDDTRDELVSFLDHVRTRDPKTICDVHEGRRDAATI
                     LMANAALESGGPVEYPA"
     gene            2067..4346
                     /locus_tag="VFV87_14075"
     CDS             2067..4346
                     /locus_tag="VFV87_14075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5104942.1"
                     /translation="MLRSILGSAAALGCIVLLAASSAPAAEAATSVGKQIASFSLEDY
                     RGKAWSLADFKDSKALVVAFVGTECPLVANYAARLQTLADQYNAAGVAFLAIDSNQQD
                     SLTELAHFARTHKIEFPVLRDAGNKVADAFGATRTPEVFLLNASRKVVYQGRIDDQFT
                     YGRQKPKVEQSYLTTAIDELVAGKPISTPHAEAVGCHIGRILTPKANSDVTYSQQIAR
                     IFQDRCVQCHRDGEIGPFTLTSYDDVVGWAQMIREVVNEQRMPPWHANPRYGHFANDA
                     SLTAEEKSLINRWVDAGAPEGDEADLPPPREFTPGWQIGEPDAIFYMAEKPYNVPATG
                     EVRYQYFMVDPGFTEDKWVKAAECRPGNRAVVHHIIVGLAAPGNQGRLKDLGGQHSEW
                     LTATAPGARPLILRDGMAKLIPAGSKLVFQMHYTPNGTVTEDRSCVGLIFADPKEVTH
                     IVATDKAATRRLDIPPQAENHHVESSHTFGSDQLMLAMFPHMHLRGKAFRYTAHYPDG
                     KQEVLLDVPAYDFNWQNSYEFAEPKLMPKGTRLECDAWFNNSESNLANPDPTATVRWG
                     DQTWEEMMIGYFDATPADGLKLPGTKNERTSQFAALLAEGKAKLNDELKALAAKSLES
                     DQGLAAFGPQLRTIAPQLDRVCWTTIEDGKLVIRRCVQEPALEKIVGGAGRKVDLRLT
                     KLATYAGETEPVVHQQLDAVRAFDLQFMSRAYASSLHIPVKIDGIAGTINFWSAEKDA
                     FPPELVSLLQEAATAMAAR"
     gene            complement(4343..5533)
                     /locus_tag="VFV87_14080"
     CDS             complement(4343..5533)
                     /locus_tag="VFV87_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5104943.1"
                     /translation="MNLARDLALALMAFVGHFALAVWLFNRLHAIPWPVRLIKILDKF
                     IILAAAAVCVVFLARWLITGQALLPRTGEVVSVSQWIWLGYAMLCWSGAIAVLPLWLI
                     PKLRERMPPALVANDSTVVDVAERIGFRPIQGLKTKILARVPGNEIFQIAVQRKTLRV
                     PSLPPALDGLSIAHLSDLHMTGHLGPEFYEVIVEETNAQKPDLIVLTGDILEKKRCLK
                     WGPPILSRLRARFGTYFIFGNHEKRLKDPGHLRQALIDAGLIDLGGRAIRTTIRDMTV
                     VLVGNELPWFGTAPMPELRTPHSAFRILLSHTPDQLPWAKSNQFDLMLAGHNHGGQIR
                     LPWIGALIVPSHYGWRYAGGLYHEPPTLLHVSRGLAGDHCIRLNCPPELALLVLTSAD
                     RQLS"
     gene            complement(5611..7320)
                     /locus_tag="VFV87_14085"
     CDS             complement(5611..7320)
                     /locus_tag="VFV87_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5104944.1"
                     /translation="MAHLRILYVCSPGDSAESLPAGIRESAEIVTVHNPLRALGKAAR
                     EKFDGVYVAADHLQSAVRLGRLLENDRILESMPDAVALLDPDQTILWANNLFLRWCNR
                     GNVVGDQFYLALGNPEVVGTDESPLRVALKTGLASGSLLKTAEGRFYQLHAAPISDPE
                     AEARHLVVSLRDVTHEALQHQKLAAIQDAGRELADLKPEEIFQMPVEERVELIKSNIL
                     HCTKDLLRFDVVEIRLLDQKTGQLVPLLEDGMDPEAAQRVLYAREQGNGVTGFVAATG
                     KSYLCEDTASDPYYLTGVAGAKSSLTVPLKLHNEVIGTFNVESPQPHAFNDADLQFLQ
                     IFCRDVAMSMNTLQLLAAQQMDAAQSCAVAIHGQVALPIDEIVTDAAFVTEHLRDIDT
                     DLRARLEKILTSARGIKEAIQQVGRTLAPTDAVPVSAGRRENLRGKRVLVVDGDRSIR
                     TSAHSLLERHDCVVETAQRGSEAESLYRHSLGDGRYHAIIAGVKLPDMSAYDLMMILK
                     PMVERLPLILTQASEWDSGHTVVKCREAGLHPAGWIIKPFKERQLLDTVETIIGWSES
                     GKV"
BASE COUNT         1487 a         2444 c         2413 g         1350 t
ORIGIN      
        1 tggcgtcgcc cgcacggtta tgttcatctg ccgcggccgc tagaaatacc tggcatcgca
       61 tatgaccacg agcgtcgatg cattcgtttt acggacgagt tcgtacagct cgacttgggc
      121 ggagtcggca agggatatgc gctcgatagc tgcgagagga ttctgactga gaatcatgtg
      181 catcacgcat tacttcatgg cggaacgagt tcggtcttgg cccggggccc ctggacaatc
      241 gcctttccag gatcggacca acggccgcgc acgctcgttg atcaatctct gtcttactct
      301 gccgtcaaca aacaagccga catcgtcgac cctgcaaccg gccagccttt gcaagcgcca
      361 gtctccgtcg ccgtcatcgc ggaatttgga atgtatgcgg agatttgttc cacagcacta
      421 ctaattgcag ggcgcgatcg cgcccacgaa atcttgtcaa gagtacacgc gcgggcggtt
      481 tggatctctg gcgatactcc gccctgttac acgagctgtt agatccccgt tttggagagt
      541 ccagatgagc ggcaatccga tgacaggtcg cagttcccgc cgttcgtttc tcgaagccgc
      601 cggcggcctc ttagccgttg ccggcgcgac tcagcttacg actgccgggt cgcagcaacc
      661 cgtgcggacg gccgtaatcg gcaccggcgc tcgcggcagc gatttgctgc gggctctgac
      721 gacgattcag ggctgcgaag tcgtggcgat cgctgacgac tatgcgccgc atctggagcg
      781 cggcgcgaag tacgccgggc cgcaggcgaa gacctacagt gactatcgca agctgctcgc
      841 cgaagcgaag ccgcaggcgg tggtcatcgc cgtgccgctg tatctgcatt atcaggtggc
      901 cagtgacgca gtggcggcgg gctgcgacgt gttcctcgaa aagacgatgt gctattcggt
      961 ggcagaatgc cgcaagctgg cggccgaagt cgagcaagcg aaatgtgtct tccaggttgg
     1021 cctgcagcgg cgggccaatc cgatctacca gcaagcggtc gcgatggtga aagcgggcgt
     1081 gatcggccaa gtctcggcga tcaagtcgca gtggcaccgc aacaacaact ggcggcggcc
     1141 ggtgccggtg cccaaaggcg acgcgaaata tggcgaactg gagcaccgcc tcaactggcg
     1201 gctttaccag cgctactcgc aggggctgat ggcggagctc ggcagccacc agatggacgt
     1261 ggcgaactgg gtgctcggca acacgcccca gcgagtcatc gccagcgggg gcatcgacta
     1321 ctggcaggac ggccgcgaag tccacgacaa tgtcttttgc atctacgact acgagctacc
     1381 gcagcgggag acggcgccgg ccggagcggg ggcgagtgcg gcccgtgggg cgggcttcca
     1441 gcctgccaag aatacggctg gcgaaccgag tggcaatgga gaatcgagtg ccgcgcccgg
     1501 cctgccgcgg agcggcggcc cacgttacac cgtccgcgtc acctactcct ccctctgcaa
     1561 caacgcctat gaaggagccg cggagctgat cctgggcacc aaaggctcgc tctttctgac
     1621 gacgaccaag gggctcttct accgcgagca actgcccgac gatccggcgt ggctcaagcc
     1681 gggtgatgcg ggcaacgccg cggtgctgac cgccggcaag acgctcaaga tgagcaactc
     1741 cccctgggcc caccgcggcg agccgctgga gatcgacctg ctggaaagcg acgacacgcg
     1801 cgacgagttg gtcagcttcc tcgaccacgt ccgcacgcgc gaccccaaga ccatctgcga
     1861 cgtccacgag ggccgccgcg acgccgccac catcctcatg gccaacgcgg ccttggagag
     1921 cggcggcccg gtcgaatatc ctgcgtaaag ctgcgtgaaa ccctctgggc atcagcgggg
     1981 tttaaggaaa gatgccagcc gctgggcttg ccggtggcgg catgacgtgc ggggacgcct
     2041 ctttacgcaa accaaagtag aaccatatgt tacggtcgat cctcggctcc gctgcggccc
     2101 tcggatgcat tgtcctattg gcggcaagct ccgccccggc agccgaggct gcaacgtccg
     2161 tcggcaagca aatcgccagc ttcagcctgg aagattatcg cggcaaggcc tggtcgctgg
     2221 cggacttcaa ggacagcaag gcgctggtcg tcgcgttcgt cgggaccgag tgcccgctgg
     2281 tggcaaatta cgcggcgcga ctgcaaacgc tggcggacca atacaacgct gccggtgtcg
     2341 cgttcctcgc gatcgactcg aaccagcagg attcgctgac tgaactggcc cacttcgccc
     2401 gcacgcacaa gatcgaattc ccggtgctca gggacgccgg caacaaggtg gccgacgcct
     2461 tcggcgcgac gcgcacgccg gaagtcttcc tactcaatgc cagccgcaaa gtcgtctatc
     2521 aaggccgcat cgacgaccag ttcacctacg gccgccagaa acccaaggtc gagcaatcgt
     2581 atctcaccac ggcgatcgac gagctcgtcg ccggtaaacc aatttcaacc ccgcacgctg
     2641 aagcggtcgg ctgtcacatc ggtcgcatac tcacgcccaa ggccaatagc gacgtgacct
     2701 acagccagca aatcgcccgc atttttcagg accgctgcgt gcagtgccat cgcgacggcg
     2761 agattggtcc ctttacgctc accagctatg acgacgtcgt cggctgggcc cagatgatcc
     2821 gcgaggtcgt caacgagcag cgaatgccgc catggcacgc caatccgcgg tacggccatt
     2881 tcgcgaacga cgccagcctg accgccgaag agaaaagcct tatcaaccgc tgggttgatg
     2941 ccggcgctcc ggaaggcgac gaagccgacc tgcctccgcc gcgcgagttc actcccggct
     3001 ggcagatcgg cgagccggac gccatcttct acatggccga aaagccgtac aacgtgccgg
     3061 cgaccggcga ggtccgctac cagtatttca tggttgaccc cggattcacg gaggacaaat
     3121 gggtcaaagc cgccgagtgc cggccaggca accgggcggt ggtgcatcac atcatcgtcg
     3181 ggctcgctgc gccggggaat caaggccggc tgaaggacct gggcgggcaa cactcggaat
     3241 ggctcaccgc gactgcgccg ggcgcccggc cgctgattct gcgggatgga atggccaagc
     3301 tgattccggc cggctcgaag ctcgtcttcc agatgcacta cacccccaat ggcaccgtga
     3361 ccgaggaccg aagctgcgtc ggcctgatct tcgccgatcc caaggaagtc acgcacatcg
     3421 tcgccaccga caaagccgcg acgcgtcgcc tcgacattcc gccgcaagcg gaaaaccatc
     3481 acgttgagtc cagccacacg ttcggcagcg atcagctcat gctggcgatg ttcccgcaca
     3541 tgcacttgcg gggcaaggcc ttccgctaca cggcccacta tcccgacggc aagcaggagg
     3601 tgctgctcga cgtgccggct tacgatttca actggcagaa cagctacgaa ttcgccgagc
     3661 cgaagctgat gcccaagggg acgcggctgg agtgcgacgc ctggttcaac aattccgaga
     3721 gcaacctggc caaccccgat ccgaccgcca cggtccgctg gggcgatcag acctgggagg
     3781 aaatgatgat cggctatttc gacgccacgc cggccgatgg cctgaagctg ccgggcacga
     3841 agaacgagcg gacgagccaa ttcgccgcgc tgctggccga aggcaaagcg aagctgaacg
     3901 acgagctgaa agctctggcg gcgaaatcac tggaaagcga tcaaggcctg gccgcctttg
     3961 gcccgcagct ccgcacgatc gccccgcagc tcgaccgcgt ttgctggacg accatcgaag
     4021 acggcaaact cgtcattcgc cgctgcgtgc aggagccggc cctggagaag atcgtcggcg
     4081 gcgcgggccg caaagtcgat ctgcggctga cgaagctggc cacctacgcc ggcgagacag
     4141 agccggtcgt ccaccagcag ctcgacgccg tccgcgcctt cgacctgcaa ttcatgtccc
     4201 gtgcctacgc gtccagcctg cacattcccg tcaagatcga cggcatcgcc ggcacgatca
     4261 acttctggag cgccgaaaag gacgcctttc cgcccgagtt ggtcagcctg ctgcaagagg
     4321 cggcaacggc gatggctgct cgctaggaca actgacgatc tgctgaggtc aggaccagaa
     4381 gcgccaactc cggtgggcag ttgagacgga tacaatgatc gcccgccaag ccgcggctga
     4441 cgtgcaggag cgtcggcggc tcgtgataga ggccgccagc atagcgccag ccgtagtggc
     4501 tggggacgat cagcgcgccg atccacggca ggcgaatctg gccgccatgg ttgtggccgg
     4561 cgagcatcag gtcgaattga ttggacttgg cccacgggag ttggtcgggg gtgtgagaga
     4621 gcagaatgcg gaaagcagaa tgcggagtgc ggagttccgg cattggcgcc gttccgaacc
     4681 agggcagctc attaccgacc aagactacgg tcatatcgcg aattgtcgtg cggatcgcac
     4741 gcccgccgag gtcgatcagg ccggcatcga tgagggcttg gcgcaagtgg ccggggtctt
     4801 tgagccgctt ctcgtggttg ccgaagatga agtacgtgcc gaagcgggcg cggagcctgc
     4861 tgagaatcgg tggtccccat ttcaagcagc gcttcttctc caaaatgtcg cccgtcaaaa
     4921 cgatcaggtc cggcttttgt gcgttggttt cctcaacgat gacctcgtag aactccggtc
     4981 ccaagtgtcc tgtcatgtgc agatcggaga ggtgggcgat cgagaggccg tccaacgctg
     5041 gcggcaggct gggcacccgc agcgttttcc gctgcacggc gatctgaaag atttcgtttc
     5101 caggcacgcg ggccaggatt ttcgtcttga ggccttggat gggccgaaaa ccgattcgct
     5161 ccgcgacatc cacgacggtc gaatcgttgg ctaccagcgc aggcggcatt cgctcgcgca
     5221 gcttcggaat caaccacagc ggcaggacgg cgatcgcccc cgaccagcag agcatcgcat
     5281 agccgagcca gatccattgg cttacggata caacttcgcc cgttcgcggc agcagcgctt
     5341 gccccgtgat cagccaccgc gcgaggaaca cgacgcaaac cgctgccgcg gccagaatga
     5401 tgaacttgtc cagaatcttg atcagccgta ccggccacgg aatcgcgtgc aggcggttga
     5461 agagccagac cgccagcgcg aagtggccga caaaggccat cagggcgagt gcgagatcgc
     5521 gagcaagatt catgtccgac gtccaacgtc cgaagtccaa agtcgcagac tccatagacc
     5581 tggcactttg gaccttggac cttggacttc ctaaaccttc ccgctctccg accagccgat
     5641 gatcgtctcg accgtgtcca atagctgccg ctccttgaac ggcttgatga tccacccggc
     5701 ggggtgcagg cccgcttcgc ggcatttgac gacggtgtgc cccgagtccc attccgaggc
     5761 ctgcgtcaga atgagcggca ggcgctcgac catcggcttg aggatcatca tcaagtcgta
     5821 agcgctcata tcggggagct tgacgccggc gatgatcgcg tgataccgcc cgtcgcccag
     5881 gctgtggcgg tagagcgatt cggcctcgct gccgcgctgg gccgtttcca cgacgcaatc
     5941 gtgccgctcc aggagcgagt gggccgacgt gcggattgac cggtcgccat cgaccactag
     6001 cacgcgcttg ccgcggaggt tttcgcggcg gcccgcgctg accggaacag catcggtcgg
     6061 ggcaagcgtc cggccgacct gctggattgc ctctttgatg ccgcgggcgc tggtcaagat
     6121 cttctccagg cgtgcccgca ggtccgtgtc gatgtcccgc aggtgttcgg tgacgaacgc
     6181 cgcatcggtt acgatctcgt cgatcggcaa cgcgacctgg ccgtgaatgg ccaccgcaca
     6241 gctctgggcg gcgtccattt gctgggccgc gaggagctgc agcgtgttca tcgacatcgc
     6301 cacatcgcgg cagaaaatct gcagaaactg caggtcagcg tcgttgaatg cgtgcggctg
     6361 cgggctttcg acattgaacg tgccgatcac ttcgttgtgc agcttgaggg ggacagtgag
     6421 cgagcttttc gcaccggcca cgcccgtcag gtaatacggg tcgctcgccg tgtcctcgca
     6481 caggtagctt ttgccggtcg cggcgacaaa tcccgtcacg ccgttgcctt gctcgcgggc
     6541 atagagcacg cgctgggccg cttccgggtc catgccgtcc tccaggagag ggacgagttg
     6601 gcccgtcttt tgatcgagca gccgaatctc gacaacgtca aatctgagca ggtccttcgt
     6661 acagtgcagg atattcgact tgatcagctc gactcgttcc tccaccggca tttgaaaaat
     6721 ttcttccggc ttgaggtcag ccagctctcg gccggcatcc tgaatggcgg ccagcttttg
     6781 atgctgcagg gcctcgtgcg tcacatcgcg gagcgatacg acgaggtgcc gggcctcggc
     6841 ttcggggtcg ctgatgggcg ccgcgtgcag ttgataaaac cgtccctccg cggttttcag
     6901 gagcgagccg ctcgccaggc cggtcttcag ggccacgcgc agcggggatt cgtcggtgcc
     6961 gaccacctcg ggattgccca aggcaagata gaactggtcg ccgacgacgt tgccgcgatt
     7021 gcaccaccgc agaaacaggt tattcgccca gagaatcgtc tggtcgggat cgagcagggc
     7081 cactgcatcc ggcatgctct ccaggatgcg atcgttctcg agcaatcgcc caagccggac
     7141 ggccgactgc aggtggtcgg ccgcaacata gacgccgtcg aacttttccc gggcggcttt
     7201 gcccagggct cgcagcgggt tgtgaacagt gacgatttcc gccgattcac gaatccctgc
     7261 cggcagcgac tccgccgagt cgccggggct gcagacgtac aagattcgca agtgggccaa
     7321 ggaattcaac tcctgccggg gcgtggagtt atgtcgctaa aattatagga cgttacatcg
     7381 catccaacaa cgcaaatggt ttgacggcta aggccgtccg attcgccctt gcgggccgtt
     7441 tgcggcgtcc aatgccctgc ctgtttccct cgctggcgac agccttcgcc gctcgaagca
     7501 ccccattcat tcgcttcctg acgatgaaat gttcgcggaa gggtggtgcg gcgcgcagct
     7561 tcgcccttcc tttgatccgg cagagcggag gtttattccc gcgttccgtt cttcgtcagc
     7621 gaggcgcaca catcgaacaa cagcggctgg ccggccaggc cacggagcag gttctcgacg
     7681 gagatttcgg ccat
//
LOCUS       DASUGB010000734         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1053978, whole genome shotgun sequence.
ACCESSION   DASUGB010000734 DASUGB010000000
VERSION     DASUGB010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053978"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFV87_14090"
     CDS             complement(<1..1077)
                     /locus_tag="VFV87_14090"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5104945.1"
                     /translation="MTSKSARRRLGGWLLASAVAATILLGLFGNHLDAVRSHQAPPHF
                     DVGYIFELLYRTLQLFVLEYNQRASQTPWPLEIARWLGAMIAGIAIFKAAEEIFHHQF
                     DDWRLRRLKEHIVVCGLGRKGLELVRQLRQRHDQVVIIEIDEGDDSLPTCRELGVTVL
                     TGDARSEHILRKAGLAKANRLLAVCKNDSDNIEIALQAREIVVSERRDRSQPLVCHVH
                     IIDLKLAELFKQHKIFTCTHDPFEARIFSFYENAARELLIRHPLELRVGETKSPGSTA
                     AMHLVIVGFGQMGESVALQAARIGHYASGQRLRLTIFDPEADQRRLNLLARYPQLEKV
                     VDLTFDSRNIEHPTVRQQLVEWADS"
     gene            complement(1188..1868)
                     /locus_tag="VFV87_14095"
     CDS             complement(1188..1868)
                     /locus_tag="VFV87_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104946.1"
                     /translation="MSSKPHADDRQFAPGATAATIPPVGWPRLLLLLVLFNAPLFFNG
                     CEFQDAKFTLGAAVPFAEFQDKLLWFSWPLMLVNGLGILAILWLATRRVSWINRAAHS
                     RWLVGALVAIALLFDSWLIWPPLWAHAVFGPQVQIAGLIAKTFEGPQGASESAVRWAW
                     LISARLYYVACVLLLGASVLGVRAFLRRYFFVRADSRWQIQLGGLMIVVVVLGTLIGL
                     VVRLIVHS"
     gene            complement(1901..>2199)
                     /locus_tag="VFV87_14100"
     CDS             complement(1901..>2199)
                     /locus_tag="VFV87_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104947.1"
                     /translation="LYRQAGGQAEFATWRLESGQESLAIFATAEAAGKYQAELPAAVE
                     YTLFQPPRDKLLAIFESCLATGIRTAALDPLAGGARTLFDLAQVIASARQQPDN"
BASE COUNT          447 a          634 c          730 g          388 t
ORIGIN      
        1 ggaatcggcc cactccacaa gctgctggcg gacggtggga tgctcgatgt tacggctgtc
       61 gaatgtcaga tcgacgactt tttcgagctg aggataacgg gccagcaggt tcaggcgccg
      121 ctggtcggct tcgggatcaa agatcgtcag gcgcaggcgc tggccgctgg catagtggcc
      181 gatgcgggcg gcctgtaagg cgacgctttc acccatttgg ccgaagccga cgatgacgag
      241 gtgcatggca gccgtcgagc cgggcgactt tgtttctccg acgcgcagct ccaagggatg
      301 tcggatcaac agttcgcggg cggcgttttc gtagaagctg aaaatgcggg cctcgaaggg
      361 gtcatgagtg caggtgaaaa tcttgtgctg cttgaacagc tcggccagtt tcagatcgat
      421 aatgtgaacg tgacaaacga gcggttgcga tcggtcgcgg cgctcgctga cgacgatttc
      481 gcgggcctgc aaggcgatct cgatgttgtc actgtcattc ttgcagacag ccagaagtct
      541 attcgctttg gcgaggccgg cctttcgtag aatgtgctcg cttctcgcgt cgccggtgag
      601 gacggtcacg cccagttcgc ggcaagtggg aagcgaatcg tccccctcgt cgatttcgat
      661 gatgacgacc tggtcgtgcc tctggcgaag ctgccgcacc agttccagtc ctttgcggcc
      721 cagcccgcag acgacgatgt gctccttgag ccgccgcagc cgccagtcgt cgaactggtg
      781 gtgaaaaatc tcctcggcgg ccttgaaaat tgcaatgccg gcgatcatcg cgccgagcca
      841 gcgggcaatt tccagcggcc agggagtctg gctagctcgc tggttgtatt cgagcacgaa
      901 aagctgcagt gtgcggtaca gcaactcgaa gatgtaccca acgtcgaagt gcggaggagc
      961 ctggtggctg cggacggcat cgagatgatt gccgaataac cccaggagga ttgtcgcggc
     1021 aaccgccgac gcaaggagcc accctcccag tcgccggcgg gcggatttcg aagtcaatgt
     1081 ttcctccagc cgcatcttgc caagggtgtt attcttgatg caggttgtgc gaattgccgc
     1141 acgccgatca cgcgcggaag gcggctattg tagcgatccg cggcacttca ggaatggacg
     1201 atcaggcgca cgacgaggcc gatcagcgtc cctaatacaa caacgacgat catcagcccg
     1261 ccgagttgga tctgccagcg cgaatcggcg cggacgaaga agtagcggcg gagaaaagcc
     1321 cgcactccga gcacgctggc gccgagaagg agaacgcaag cgacgtaata gagccgggca
     1381 ctgatgagcc aggcccagcg gacggctgat tcgctcgcgc cttgcggccc ttcgaacgtt
     1441 ttggcgatga gcccggcaat ttggacttgc ggcccaaaga cggcgtgcgc ccacagcggc
     1501 ggccagatca gccacgaatc aaagagcaga gcgatggcaa cgagggcgcc tacaagccag
     1561 cgcgagtgcg ccgcgcggtt gatccatgag acacggcgcg tagccagcca gaggatcgca
     1621 agaattccca gcccgttcac cagcatcagg ggccaagaga accacaagag cttgtcttgg
     1681 aattccgcga aggggacggc cgcgccgagc gtaaatttcg cgtcctggaa ctcgcagccg
     1741 ttgaagaata gcggcgcatt aaacagcacg agcaggagga gcaggcgggg ccaaccgacg
     1801 ggcggaatcg tggccgcggt tgcgccggga gcgaactggc ggtcgtcagc gtgcggtttg
     1861 gacgacattc cttgaatcta ccgctgcgtg cggccggcga tcaattgtcg ggctgctgcc
     1921 gggcggaggc gatgacctga gcaaggtcga acagcgttcg tgcgccacca gcgagcgggt
     1981 cgagggctgc ggtgcggatg ccggtagcca gacagctttc gaagatcgcc agcagcttgt
     2041 ctcgcggcgg ctgaaagagg gtgtactcaa cggcagccgg tagctcggct tgatatttcc
     2101 cagcggcttc cgcggtagcg aagatcgcca gcgattcctg gccgctctcc aagcgccagg
     2161 tggcgaactc ggcctgcccg cccgcctgtc gataaagca
//
LOCUS       DASUGB010000735         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_498955, whole genome shotgun sequence.
ACCESSION   DASUGB010000735 DASUGB010000000
VERSION     DASUGB010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498955"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFV87_14105"
     CDS             complement(<1..690)
                     /locus_tag="VFV87_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104948.1"
                     /translation="MSASANRREFLQAASAGAIAATTLSATAVRAANAASDKLVVGLV
                     GCGGRGTHDANLFKSVPNVELAYACDVDASRRENIAKNLGLPTSRIVSDMRRILDDKT
                     VDIVIVTTPDHWHSPASILAVGAGKHVYVEKPISHNIREGRLLVEAAARNKRHVQHGT
                     QCRSTSMMIEAVKQLREGIIGTVLVAKCWNIQKRGSIGRGQDTAPPAGLDYESWIGPA
                     TMIPYRTNRVHN"
     gene            complement(735..1721)
                     /locus_tag="VFV87_14110"
     CDS             complement(735..1721)
                     /locus_tag="VFV87_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5104949.1"
                     /translation="MQYRPLGKTGLSISAISFGAGPISQLLVGHDADRQRAVIQHAID
                     RGINWFDTAATYGNGQSEQNLGRVLNELNAVHRVHIATKVRLTPEDLADIPGAIRRSV
                     TASLERLRLPRVTLLQLHNSITKERGDEPSSITPQDVLGPAGVAKTFERLRFEGVALH
                     LGLTGLGQPAALREVVASGRFETMQVPYQLLNPTAGWEFRGREREANYGNIISDCALQ
                     GMGVLAIRVFAGGALLGNPPSPHTLKTPFFPLALYQRDGRRAELLKSILGPELPLAQA
                     AVRFALAHPFVHSALTGFCRIGEIDEALQALDAGPSRIFWDDVLAALDAQAH"
     gene            1828..2910
                     /locus_tag="VFV87_14115"
     CDS             1828..2910
                     /locus_tag="VFV87_14115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5104950.1"
                     /translation="MQTRVTRRNVLQGALGAGAALALAGSPRWSRAAGTERIKVGQIG
                     VGHAHATKLEVYRRSGDYEVVGVVEPDKELRERAAGAAAYRDLPWMTEEQLLNVSGLQ
                     AVLVETRVRDLLDTAGRCVAAGKHIHLDKPAGDSLPQYRALLDAAAKRKLVVQMGYMF
                     RYSPAVVLMRKFLNEGWLGDVFEVHTVMSKVVPDGQRRELAEFKGGIMFELGCHVIDL
                     VVGVLGRPERVHAFPRHSGPQSDGLADNMLAVLEYPEATASVKSSALEVNGGARRHFV
                     VCGRSGTFHIQPLDAPNVTYTLDRDRGEYQRGTHEIKFGNYERYVGDAADMAKILRGE
                     KECDFSYSHDLAVQETVLLAGGMPIV"
     gene            complement(3002..>3219)
                     /gene="purH"
                     /locus_tag="VFV87_14120"
     CDS             complement(3002..>3219)
                     /gene="purH"
                     /locus_tag="VFV87_14120"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680318.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5104951.1"
                     /translation="IAIQKAGERATGSVLASDAFFPFPDSIHRAAAAGIAAIIQPGGS
                     VKDEEVIAAANQRGIPMIFTGRRHFKH"
BASE COUNT          543 a          980 c         1132 g          564 t
ORIGIN      
        1 gttgtgcacg cggttggtgc ggtacggaat catcgtcgcc gggccgatcc agctctcgta
       61 atcgagaccg gccggcggcg cggtatcctg cccgcggccg atcgagccgc gcttctggat
      121 gttccagcac ttggcgacga gcacggtgcc gataatccct tcacgcaact gcttcaccgc
      181 ctcgatcatc atgctggtgc tgcggcattg ggtgccgtgc tggacgtgcc gcttgttgcg
      241 ggccgcggct tcgacgagca gcctgccttc gcggatgttg tgcgagatcg gcttttcgac
      301 atagacgtgc ttgccggcgc caacggccag gatcgatgcg ggcgaatgcc agtggtcggg
      361 cgtcgtcacg attacaatat cgaccgtctt gtcgtccagg atccgccgca tgtcggagac
      421 gatgcggctg gtgggcaggc cgaggttttt cgcaatgttc tcgcggcggc tggcatcgac
      481 gtcgcaggca taggccagct cgacgttcgg cacactcttg aacaagttcg cgtcgtgcgt
      541 gccgcgcccg ccgcagccga cgaggccgac cacgagctta tcgctggccg cattggcggc
      601 gcgaacggct gtcgccgaaa gcgtggtggc ggcaattgcc cctgcggagg cggcttgcag
      661 gaattcgcgg cgattggcgc tggcggacat ggcggttctc cggtgaaaaa gcgacgcctc
      721 gcttgcgcgt cgggttagtg tgcctgggca tcgagggcgg ccagcacgtc gtcccagaat
      781 atacgcgatg gccccgcgtc cagcgcctgg agggcttcat cgatctcgcc gatccggcaa
      841 aagccagtca gcgccgaatg gacaaacgga tgggcaagcg caaagcgaac cgctgcctgc
      901 gcgagcggca attcggggcc caggatgctc ttgagtagct cggcccgccg gccgtcgcgc
      961 tggtagagag ccagcggaaa gaagggggtc ttcagcgtgt gcgggctggg cggattgccc
     1021 agcaatgccc cgccggcaaa gacgcggatc gccagcacac ccattccctg caaggcgcag
     1081 tcggaaatga tattgccgta gttcgcttcc cgctcgcgtc cgcgaaactc ccagccggcg
     1141 gtcggattca gcagttgata gggcacctgc atcgtctcga agcggccgga cgcgacaacc
     1201 tctcgcaggg ccgcgggctg gccgaggccg gtcaggccca gatgcagtgc gacgccttca
     1261 aaccgcagcc gctcgaaggt cttcgccacg ccagccggcc ccagcacgtc ctgcggcgtg
     1321 atcgacgacg gctcgtcgcc gcgctccttg gtgatcgaat tgtggagctg caggagcgtc
     1381 acccgcggaa ggcgcaggcg ctcgaggctg gcagtgaccg agcggcgaat ggcgccggga
     1441 atatccgcca gatcttccgg cgtcagacgg accttcgtcg cgatatgcac gcggtgaacg
     1501 gcgttcagct cgttcagcac acgccccagg ttctgctcgg attgaccatt gccgtaggtg
     1561 gcggccgtat cgaaccagtt gattccccgg tcgatcgcat gctggatcac ggctcgctgg
     1621 cggtccgcat cgtggcccac gaggagctgc gagatcggcc ccgcgccgaa cgagatggcc
     1681 gaaatcgaga ggccggtctt gccgagcggg cggtattgca tggtgggacg tatctgctgg
     1741 aagccgaagg gttgagcttc ggctatgata cccgcggcca gtgccggccg gttcccgcag
     1801 ccagttcact tactcgacga ggaagccatg cagacccgag tcacacgacg taacgtgctc
     1861 cagggtgctc ttggcgctgg tgccgccctg gcgctggccg gttcgccgcg ctggtcacgg
     1921 gcggccggaa cggagcggat taaagtcggc cagatcggcg tgggtcatgc tcatgcgacc
     1981 aagctcgagg tctatcggcg ctccggcgac tacgaggtcg ttggggtcgt cgaaccggac
     2041 aaagagctgc gcgagcgggc cgccggtgcg gcggcgtatc gcgacctgcc gtggatgacc
     2101 gaagagcaac tgctgaacgt gtcgggactg caggcggtgt tggtcgagac gcgcgtgcgg
     2161 gacttgctcg atacggctgg gcggtgcgtg gcggccggca agcacatcca tctggataag
     2221 ccggccggcg attcgctgcc gcagtatcgg gcgctcctcg acgctgcggc gaaacgcaag
     2281 ctcgtcgtgc agatgggcta tatgtttcgc tatagcccgg ctgttgtgct gatgcggaag
     2341 ttcctgaacg agggctggct gggcgacgtg ttcgaagtcc acacggtgat gagtaaggtc
     2401 gtgccggacg gccagcggcg ggaactggcg gaattcaagg gcgggatcat gttcgagctg
     2461 ggctgccacg tgatcgacct ggtcgtcggc gtgctgggcc ggccggagcg agttcacgcc
     2521 ttcccgcgcc acagtggacc gcaaagcgac ggcctggccg ataacatgct ggcggtcctg
     2581 gagtatccgg aagccacggc gagcgtgaag tcctcggcgc tggaagtgaa cggcggcgcg
     2641 cggcgtcatt tcgtcgtctg cggacgcagc ggcacgtttc acattcagcc gctggacgct
     2701 ccgaatgtga cgtacaccct cgatcgcgat cgcggcgagt atcagcgcgg cacgcacgag
     2761 atcaagttcg gcaactacga gcggtatgtc ggggacgcgg ccgacatggc gaagattctg
     2821 cgcggcgaga aggagtgcga cttctcgtac tctcacgatc tggccgtgca ggagacggtg
     2881 ctactggccg gcgggatgcc aatcgtttga catcgtttaa cgcgcagccg aaggcgtagc
     2941 agattaaccg cgcgatacgg catcgcctgc ggctcgccgt taaacgagcg cccgcgctcc
     3001 gttagtgctt gaagtgccgg cggccggtga agatcatcgg gatgccgcgc tggttggcgg
     3061 cggcgataac ctcttcgtcc ttcacgctgc cgccgggctg gatgatcgcg gcaatgccgg
     3121 cagcagcggc gcggtggatc gaatcgggga aagggaaaaa ggcatcggac gccaggaccg
     3181 agcccgtggc ccgctcgccc gccttctgga tggcgattt
//
LOCUS       DASUGB010000736         4894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_80450,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000736 DASUGB010000000
VERSION     DASUGB010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80450"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFV87_14125"
     CDS             complement(<1..723)
                     /locus_tag="VFV87_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104952.1"
                     /translation="MTVFLLSMILIGQAEPAPAPDDELAKQQQAKWNALYAKIAAEYE
                     IKLGKEGKNQLQLVPEAVLNWSNPVRGGETNGSVFVWTYDGRTEAVGTIFSHLVRDDP
                     NQKVTAHSFQSLSQQPLAGQRPGRPGAWSLDSAGVSPQKIPGAPAPGRSRAARLAQMR
                     ELVREFSATTTLEGVEQELRLLTQPLHRNEKSTGDVLDGALFTFVTGTDPELMLVIEA
                     RADEGTDAPTWHYAAGRFTDLTL"
     gene            complement(1082..1168)
                     /locus_tag="VFV87_14130"
     tRNA            complement(1082..1168)
                     /locus_tag="VFV87_14130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1132..1134),aa:Ser,seq:tga)
     gene            1313..2746
                     /locus_tag="VFV87_14135"
     CDS             1313..2746
                     /locus_tag="VFV87_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5104953.1"
                     /translation="MSAAPVTLDEAAPRPQLSLVDTTSIIVGIIIGSAIYESSPGIAS
                     GAGKWAESLALSVKAAPEQAQGAAMAALVGVWVAGALIALVGALCYAELATAYPRAGG
                     TYVFLTEAFGKNVGFAFAWAEFWIVRPGNVGAIAFVLANYARQILAPQAKGDPVLEVG
                     LAVAAIVVLTLVNAAGLRAGKWTQNLLTAGKLLGLAAIVVTAFTLPRPTAAAPIVAEK
                     FQTIGFALIMVMFAYGGWTDMSFVAAEVRHPERNIFRALVLGTSVVAAIYLALTLAFV
                     WALGIGGLTQSSAVAAEVVKLRFNNAGAVAISVLVVVSCLGAINGMLFTGARVYYALG
                     TEHPVFRWLGTWDGRSGMPLRSLAVQSAVTVALVVGFGQYEGGFGRLVVFTAPFYWAF
                     IALVGVALIVLRVRSAIAAATFRAPLFPLTPAIFALSCGAMVYASLDFAYRNRAWEMA
                     WAIAVVLAGVIVGLVDHAAFRHSAAHR"
     gene            2897..>4894
                     /locus_tag="VFV87_14140"
     CDS             2897..>4894
                     /locus_tag="VFV87_14140"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5104954.1"
                     /translation="MSTAASIDSAQAAPGPAMRRGGGAARIEPGSPATGTPDPHLVAE
                     MKQEIRVLVQEIAELAAGDLSPEEFYSGFLARVVSAMAAVGGAIWITGESGKLKLACQ
                     VNLAQTGVERTPQDRTRHGLLLKNVMSGAQAVIAPPASGTAAAEAGNPTEHSLVLAPL
                     VVERKSEAVVEIFQRAGGGPSTQRGYLRFLVQMCDVACDYLKNQRLRQLASSQSLWQQ
                     IEQFTQALHRSLDVEATAYTVVNEGRRLIGCDRVSVALAYGSRCRVEAVSGLDSIDRR
                     ASEVRRLAKLAEAVLRTREPLWSQAGQEDLPEQIDEPLQAYVDQAHSRLVAVLPLVKP
                     TDGEPASAPIGALIVEQLREAKVGDSLRSRSELAAQHASAALANAIEHSSLPLLSLWK
                     AAGQATWLLRGRALPKTLLAVGLLIFGIAALALVPTDFEVAARGKLQPAERREVFAPL
                     DGVVAQVAVRHGELVEAGSILAELTNTDLELQLASLIGRQTTNAERLAALQRALLDTR
                     NGMSRLTAAEENRLAGEMLELRQEAESIERELALVRQKQQQLTIVAPQRGQVVTWKVE
                     DLLLHRPVQRGQGLLTLANPDGPWELELYLPERRIKHVQAQSQLDATFALASHPGQTF
                     RGRVVEIEQSAEVRGDEGNTVLVRVAVEKEELPPLFDQTTVT"
BASE COUNT          812 a         1492 c         1663 g          927 t
ORIGIN      
        1 aagcgtcaga tcggtgaacc ggccagcggc atagtgccac gtcggggcat cggtcccttc
       61 atccgctcgc gcttcaatca ccagcatcag ttctggatcg gtccccgtca caaacgtgaa
      121 cagcgctccg tcgagcacat ctccggtgct cttttcattg cgatgcagcg gctgggtgag
      181 cagtcgcaat tcctgctcaa caccttccaa cgtcgtggtg gccgagaact ctcgcaccaa
      241 ctctcgcatt tgtgccaggc gggcggctcg gctacgtccc ggtgccggtg cgccaggtat
      301 cttctgcgga ctgactcccg cactgtccaa cgaccaagcg ccaggcctgc ccggccgctg
      361 gccagccaac ggttgctgag agagcgactg aaaggaatga gctgtgacct tttggttggg
      421 gtcgtcgcgc acgaggtggg agaagattgt acctacggct tccgttcgac catcgtaggt
      481 ccaaacgaat acggagccat tagtttctcc tccgcgaacg ggattgctcc aattcagtac
      541 cgcctcgggc acgagctgaa gttggttctt tccctccttg ccgagcttga tctcatactc
      601 ggcagctatt tttgcataaa gtgcattcca cttggcctgc tgttgcttgg ccagttcgtc
      661 gtcgggagca ggtgcaggtt cggcctgtcc aatcaggatc attgacaaaa ggaaaacggt
      721 catgcggcac ctcggcgtgc cagtatcaga cgtaatacct taaaacgcaa tcgcaccacc
      781 acagtcagcc gcagcacgtc gcggtttgtg aaacggagca tgggcggctt atggggacgg
      841 caagcaacgc aagcggtcgt cctgggaaac gactttcgcg tcgtccgcag ccactgctct
      901 gaaccctggc ccttcggttg gcggtggcgg atgaccgcac aagtcgaatc gtaccggcgg
      961 gtaccactcg gaacatagtc tccccaagtg ccaggggcgg taattcctcg taaggatttg
     1021 caggcggcct ccagaattcc cgggaatatc gagctggcgc aaattcgtgc gacgccttta
     1081 gcggatgggg agggattcga acccccggtg cggttgcccg cacgccggtt ttcaagaccg
     1141 gtgcattcga ccactctgcc acccatccgg ctggcggcag ctcaattcta gtttgtcagc
     1201 cgttgggcga aaacatcgag gagcgaactc gacgaaggac aggcttggga atggtaaagt
     1261 tcgcgttgaa cgcggctacg tgatttagga gatcgtccgc tacactttgg gcatgtccgc
     1321 tgcacctgtt acgctcgacg aagccgcgcc caggccgcag ctttcgctgg tcgatacgac
     1381 gtcgatcatc gtggggatca ttattggctc ggccatctat gagagttcgc cggggattgc
     1441 ttccggtgcg gggaagtggg cggaaagcct ggcactctcg gtgaaggcgg cgcccgagca
     1501 ggcgcagggc gctgccatgg cggctctggt cggagtttgg gtggcggggg cgctgatcgc
     1561 gctggtgggt gcactgtgct atgcagagct ggcgactgcc tatccgcggg ccggcgggac
     1621 gtatgtgttt ctaacggaag cctttgggaa gaatgtcggc tttgcgttcg cctgggccga
     1681 gttctggatc gtgcggccgg gaaatgtggg ggcgatcgcg tttgtgttgg ccaactacgc
     1741 ccgccagatt cttgcgccac aagcgaaggg cgacccggtg ctcgaggttg gtctggcggt
     1801 cgccgcgatc gtcgtcctca cgctggtcaa cgctgcgggg ttgcgggccg gcaaatggac
     1861 gcagaacctc ctgacggcgg gcaagctgct ggggctggcg gcaattgtcg tcacggcatt
     1921 cacgcttccc cggccaaccg ccgccgctcc gattgttgcc gaaaagttcc agacgatcgg
     1981 cttcgccctc atcatggtca tgttcgccta tggaggctgg accgatatgt cattcgtcgc
     2041 tgccgaggta cggcatccgg agcggaacat tttccgcgcg ctggtgctgg ggacgtcggt
     2101 tgtggcggcg atctatctgg cgctgacgct ggcgtttgtg tgggcgctgg ggatcggcgg
     2161 gttgacacag tcgtctgcgg tcgctgccga agttgtcaaa ctgcggttca acaatgccgg
     2221 agcggtggcc atcagcgtgc tggtcgtcgt ttcgtgcctg ggggcgatca acggaatgct
     2281 cttcaccggc gcgcgagtct attacgccct cggcacggag caccccgtat ttcgctggct
     2341 gggcacttgg gatggccgca gtggcatgcc attgcgatcg ctcgctgtgc agtcggcagt
     2401 cacggtcgcc ctcgtcgtcg gctttggcca atatgagggc ggattcggcc ggctggtggt
     2461 gttcacggcg ccgttctact gggcgttcat cgcgctggtt ggcgtggccc tcatcgttct
     2521 gcgggtgcgc tcagcaattg ctgcggcgac gtttcgcgct ccgctgtttc cgctgacgcc
     2581 ggcaatcttc gcgctgtcgt gcggcgcgat ggtctatgcc tcgcttgatt ttgcctatcg
     2641 caatcgggca tgggaaatgg cgtgggctat cgccgttgtg ctggcaggcg taattgtcgg
     2701 cctggttgat catgccgcgt tcaggcactc cgctgcccac cgctagcagg ccactcgctg
     2761 cgccggtccg gcaaactctg cggcctgtgc cggcggtagc accagacgcg actctgcggc
     2821 tgccgaagaa gatgcgtggg gcaagcagga atgcctgcca cgtttgccgc gacctcccgc
     2881 gaacccgctg aggggaatga gcacagccgc ttcgatcgat tccgcgcaag cggctcctgg
     2941 tcccgcgatg cggcgcggcg gcggcgctgc gcgcatcgag ccgggcagtc ccgccaccgg
     3001 cacgcccgat ccgcacctgg ttgcggaaat gaagcaggaa atccgcgtcc tggtgcagga
     3061 aatcgcggaa ctggcggcgg gcgatctctc gccggaggag ttttactcgg gattcctggc
     3121 ccgggtcgtg tcggcgatgg ctgccgtcgg cggggcgatc tggatcaccg gggaaagcgg
     3181 caagctcaag ctggcctgcc aggtgaatct cgcacagacg ggcgtggagc ggacgccgca
     3241 agaccgcacg cgacatggcc tgctcttgaa gaacgtgatg agcggcgcgc aggccgtgat
     3301 cgcaccaccg gcgagcggca cagccgccgc cgaggcgggc aatccgaccg aacactcact
     3361 cgtgctggcg ccgctggtgg tggagcgcaa gtcggaagcg gtcgtcgaaa tcttccagcg
     3421 ggccggcggc gggcccagca cgcagcgcgg ctatctgcgg tttctggtgc agatgtgcga
     3481 cgtcgcctgc gactacctga agaaccagcg gctgcggcag ctcgccagca gccaatcgct
     3541 ctggcagcag atcgagcagt tcacgcaggc cctgcaccgc tcgctcgatg tggaggcgac
     3601 cgcctatacc gtcgtgaacg aagggcggcg actgatcggc tgcgaccgcg tttcggtggc
     3661 cctggcctat ggcagccgct gccgcgtgga ggcggtcagc gggctcgatt cgatcgaccg
     3721 gcgggcgagc gaagtgcggc ggctggcgaa gctggccgag gcggtccttc gcacgcggga
     3781 gccgctgtgg agccaggccg gccaggaaga cctgcccgag cagatcgacg agccgctgca
     3841 ggcgtatgtc gatcaggcgc attcgcggct ggtggcggtg cttccgctcg tgaagccgac
     3901 cgacggggaa cctgcgtcag caccgatcgg cgccctgatc gtcgaacagc ttcgtgaagc
     3961 gaaggtcggc gattcgctgc gttcgcgcag cgaattggcc gcgcaacatg cttcggcggc
     4021 gctggccaac gccatcgagc acagtagttt gccgctgcta tcgctgtgga aagctgcggg
     4081 gcaggcgacc tggctgctgc gcggcagagc gctgcccaag acgctgctcg cggtcgggct
     4141 gttgatcttc gggatcgctg cgctggccct tgtgccgacc gacttcgagg tcgccgcccg
     4201 cggcaagctg cagccggccg agcggcgcga agtcttcgct ccgctcgacg gcgtcgtggc
     4261 tcaggttgcg gttcgacatg gcgagctggt cgaagccggg agcatcctgg cggagctgac
     4321 gaacaccgat ctggaactgc agctcgcctc gctcattggc cggcagacca cgaatgccga
     4381 acggctggcc gccctgcaac gcgcgctcct ggacacgcgg aatggcatgt cgcgcctgac
     4441 ggcggcggaa gagaatcgcc tggcgggcga gatgctcgaa ttgcggcagg aagccgagag
     4501 tatcgagcgc gagctggcgc tcgtgcgcca gaaacagcag cagctcacga tcgtcgcgcc
     4561 gcagcgcggg caggtcgtga cgtggaaggt cgaggacttg ctgctgcatc gcccggttca
     4621 gcgcgggcag ggcctcttga cgctcgccaa tcccgatggc ccgtgggagt tggagttgta
     4681 cttgcccgag cggcggatca agcatgtcca agcgcagtct cagcttgacg cgactttcgc
     4741 cctcgccagc catcccggcc agaccttccg cggccgcgtc gtcgaaatcg aacaatccgc
     4801 ggaagtccgg ggcgatgaag gcaacaccgt gctggtccgc gtggcggtcg agaaagaaga
     4861 gctgccgccg ctgtttgacc agaccaccgt cacc
//
LOCUS       DASUGB010000737         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_776439, whole genome shotgun sequence.
ACCESSION   DASUGB010000737 DASUGB010000000
VERSION     DASUGB010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776439"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..687)
                     /gene="grpE"
                     /locus_tag="VFV87_14145"
     CDS             complement(97..687)
                     /gene="grpE"
                     /locus_tag="VFV87_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5104955.1"
                     /translation="MTEQQEKTKREAAGNGNAHAPPEAALVAGEAAERKIADLEAQLR
                     EETQRALRAQAELENYRKRVQREVSDERRYAALPLVRDLLPAIDNLQRAIDATPTRSV
                     SEDASKSTVESNAVLLEGVKMVVAQLETVLKQHHCQPIEAAGQPFDPNQHEAIAQEPS
                     SEYPAGTVTRAVQAGYKLHDRVVRPAQVFVSTGPEK"
     gene            complement(694..1860)
                     /gene="dnaJ"
                     /locus_tag="VFV87_14150"
     CDS             complement(694..1860)
                     /gene="dnaJ"
                     /locus_tag="VFV87_14150"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5104956.1"
                     /translation="MASKRDYYEVLSVKRDASDGQISAAYRKLAVKYHPDSNPGDAEA
                     VEKFKEAAEAYEILSDPEKRARYDRFGHQGVEQMGGTHFHDVEDIFEAFGDIFSEMFG
                     GGAGRRGGRRVRRGADVRADVTLDLEEAARGVVKSVEFNRSKACNTCNGTGSRPGSQR
                     ATCRRCNGHGQIVQSAGILRVQTTCPTCQGAGSVITDPCESCRGRGYVQERAKLDVTI
                     PAGIDDGMRVRLTGYGEPSPDGGPPGDCYCFVRVRPHKIFQRDGAHLILQMPITYSQA
                     ALGASIEVPTLQGPHELTLPAGTQSGAVFRIRGQGLPDPRGGAKGDLHVQTFIEVPKR
                     LTGEQERLLRELAELEQTEVAPHRKSFLERIRAYFQPSDGESSEAQPSARSAKE"
     gene            complement(2114..>3006)
                     /gene="groEL"
                     /locus_tag="VFV87_14155"
     CDS             complement(2114..>3006)
                     /gene="groEL"
                     /locus_tag="VFV87_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5104957.1"
                     /translation="KPLLIIAEDVDAEALTLLVVNKLRGVLNVCAVKAPGFGDRRKAL
                     LGDIAVLTGGTLISEDLGIQLENLTLEHLGRAKKVTVDKNNTTLVEGAGKRADIDKRI
                     QQIKNQIAQTESEYDKEKFQERLAKLAGGVAVISVGAETEADMKQKKARVEDALHATR
                     AAVEEGILPGGGVALLRCTDAVEKLRDSLKGDEKVGADIILRVLPSPLVTIVNNGGKD
                     GSVVADEVCQKSGNQGYDANSGEYVDMYKAGIIDPVKVVRTALGNAASIAALLLTTEA
                     LVTNFDKDDKAKRAVEGAVR"
BASE COUNT          418 a          949 c         1015 g          624 t
ORIGIN      
        1 ggcggcaggc gtcgcaggcg tagtcgtagg taggcattac taggttgagc gtcgaaggtc
       61 gaaggtcatc gttcggtcga aggtccttcg acagtcctat ttttctggtc ctgttgaaac
      121 aaaaacctgg gccgggcgca cgacccggtc gtgcagcttg tagccggctt gtacggcgcg
      181 ggtgaccgtt ccggcggggt attcgctgct cggttcctga gcgatggctt cgtgctggtt
      241 gggatcgaat ggctggcccg cggcttcgat cggctggcaa tggtgctgct tgaggaccgt
      301 ttcgagctgg gcgacgacca ttttcacgcc ttcgaggagg acggcattgg attccaccgt
      361 tgatttgctc gcgtcctcgc ttacgcttcg ggttggtgtg gcgtcgatcg cccgctggag
      421 gttgtcgatc gccggcaaga ggtcgcggac cagcggcagg gcggcgtagc ggcgctcgtc
      481 ggatacttcg cgctgcaccc gtttgcggta gttttcgagc tcggcctggg cgcggagggc
      541 ccgctgcgtc tcttcccgaa gctgagcttc caggtcggcg atcttccgct cggctgcttc
      601 gccagcgaca agcgcggcct cgggcggcgc gtgggcgttg ccgtttccgg cggcttcgcg
      661 tttggtcttt tcctgctgct cggtcatgtg cggttactcc ttcgcggagc gtgcggatgg
      721 ctgcgcctcg ctcgactcgc catcggaggg ctgaaaatag gcacgaatgc gttccaggaa
      781 cgacttgcgg tgcggggcga cctccgtctg ctccagttcc gctaactcac gcaaaagccg
      841 ttcctgttct cccgtcagcc gttttggcac ttcgatgaac gtctgcacgt gcaagtcgcc
      901 cttcgccccc ccgcggggat cgggcaggcc ctggccgcgg atgcggaaga cggcgccgga
      961 ctgtgtaccg gccggcagcg tcagctcgtg cgggccttgc agggtcggca cttcgatgga
     1021 ggcgcccagc gccgcctggc tgtaggtgat cggcatctgc aggatcaggt gcgccccgtc
     1081 ccgctgaaaa atcttgtgcg gccggacccg cacgaagcag tagcagtcgc cgggcgggcc
     1141 gccgtcgggg ctcggctcgc catagccggt caggcggacg cgcatgccgt cgtcgatgcc
     1201 cgcggggatc gtcacgtcga gcttggcccg ctcctgcacg tagccgcggc cgcggcagct
     1261 ctcgcacgga tcggtgatga ccgagcccgc gccctggcag gtgggacagg tggtctggac
     1321 gcggaggatg ccggccgact gcacgatttg cccgtggccg ttgcagcggc ggcaggtcgc
     1381 ccgctgtgag cctggccggc tgccggtgcc gttgcacgtg ttgcaggcct tgctgcggtt
     1441 gaattcgaca cttttgacga caccgcgggc cgcctcttcc aggtcgagcg ttacatcggc
     1501 ccggacgtcg gctccgcggc gcacccgcct gccgcctctt cggccggctc cgccgccgaa
     1561 catctcgctg aagatgtcgc cgaaggcctc gaagatgtct tccacgtcgt ggaagtgcgt
     1621 cccacccatc tgctcgaccc cctgatgccc aaagcggtcg tagcgggctc gcttttcggg
     1681 gtcggagaga atctcgtagg cctccgctgc ctctttgaat ttctcgaccg cttcggcgtc
     1741 gcccgggttg ctatcggggt gatatttcac cgccagtttg cggtacgcag ccgagatctg
     1801 gccgtcggaa gcatcccgct tcacggacaa gacttcgtag taatcgcgtt tggaggccat
     1861 cgtgggcatg gcaggaacgg tgggctcaga tcaccgggaa atgtacgtca tgcgcaattg
     1921 ttcggggatg gcaaaaaatc ggccacctcg caagcgaagt ggccgggaga ggctggtttt
     1981 ctcatgttcc tagccgggac caaacctggt cccggagaac tgccgacgcc gcgtccatcc
     2041 cgctgctccg ggacctgtta ggtcccggct aaggatcgga accgcgacca atggtcgcgg
     2101 ctttggccat ggtttaccga acggcgcctt ccacggctcg cttggctttg tcgtccttgt
     2161 cgaagttagt cacgagggcc tcggtcgtca gcaacagagc cgcgatgctg gcggcgttgc
     2221 cgagggcggt gcggaccacc ttcaccgggt cgatgatgcc ggccttgtac atatcgacgt
     2281 actcgccgct gttggcgtcg tagccctggt tgcccgactt ctggcagacc tcgtcggcga
     2341 cgacggagcc gtccttgccg ccgttgttga cgatggtgac gagcggcgag gggagaaccc
     2401 gcaggatgat gtcagctccg accttctcgt cccccttgag ggagtcgcgg agcttctcga
     2461 ccgcatcggt gcagcggagc agggcaacgc cgccgccggg aaggatgcct tcttcgacag
     2521 ccgcacgggt ggcgtgcagg gcgtcttcca cgcgggcctt cttctgcttc atgtcggctt
     2581 cggtctcggc accgacgctg atgaccgcca cgccgccagc gagcttggcc aggcgttcct
     2641 ggaacttctc cttgtcgtac tcgctttcgg tctgggcgat ctggttcttg atctgctgga
     2701 tccgcttgtc gatgtcggcc cgcttgccgg ccccttcgac gagcgtcgtg ttgttcttat
     2761 cgacggtcac cttcttggcg cggcccaggt gttccagcgt gaggttctcg agctggatgc
     2821 ccaggtcctc gctgatcagc gtgccgcccg tcaggacggc gatgtcgccc agcagggcct
     2881 tgcggcggtc gccgaagccc ggggccttca ccgcacagac gttgagcacg ccgcgaagct
     2941 tgttgaccac caagagcgta agcgcctcgg catcgacgtc ttcggcgatg atgagcagcg
     3001 gcttgc
//
LOCUS       DASUGB010000738         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_221783, whole genome shotgun sequence.
ACCESSION   DASUGB010000738 DASUGB010000000
VERSION     DASUGB010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221783"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="VFV87_14160"
     CDS             <1..1994
                     /locus_tag="VFV87_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5104958.1"
                     /translation="ARLPADGPADNNPETVRRVPKLGIEVPADRRARLEAELDFLKTS
                     IDRLRRLDKEAHPQMAELLPDVEVYYKAVHDALTYQEFFDSKEIDFAEQALATAKERA
                     DQLSAGQAPWTNATGRVVRGYVSKIDGSVQPYGLIVPESYTTKGPGRFRCDLWFHGRG
                     ETLSELNFVRDRTNNPGQFTPEDTIVLHPYGRYCNAFKFAGEIDVLEALEAVKRHYRV
                     DEDRIGERGFSMGGAAAWQFAVHYADRWYAANPGAGFSETPEFLRIFQQEELQPTWWE
                     QRLWHWYDCPDWCLNLRHCPLVAYSGELDTQKQAADIMQAALAEQGIDLLHLIGPQTR
                     HAIHPQSKIEIESRMQSLARAGRDWQPLDIEFTTYTLRYNRMHWIAIDGLQEHWQRGH
                     VSASLLAGEVVIDCGGVSDMTLSFPAGECPLDVQSPVIVTIGEQELEAPAPKSDRSWN
                     VSLHKIRGEWRLGKRPGNELRKQPGLQGPIDDAFLDSFLFVRPTGSAWHSPVGNWAKS
                     EMERAVEHWRRHFRGIARVKDDRAVTPDDIAGSNLVLWGDPASNSLLKQIAGKLPIRW
                     TEQQIEAGDEKFPATNHALIAVYPNPLNPRRYVVLNSGFTFRDYAYLNNARQVPMLPD
                     WAVVDLSTPPGSVWPGKIAAADFFDERWQLKTSPERSKH"
     gene            complement(2134..2295)
                     /locus_tag="VFV87_14165"
     CDS             complement(2134..2295)
                     /locus_tag="VFV87_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104959.1"
                     /translation="MHHKHQGRSDPADFFKLDDRHHPSEKTRKQQWLPAKRTTNEEKP
                     LDLLGMSGL"
BASE COUNT          478 a          787 c          748 g          436 t
ORIGIN      
        1 ctgcccgcct gccggctgat ggaccagccg acaataatcc ggaaacggtc cgccgggttc
       61 ccaagctcgg catcgaagtg ccggccgacc gccgggcgcg actggaagcg gagctggatt
      121 ttctgaagac ttcgatcgat cgactgcggc gtctggacaa ggaggcgcat ccgcagatgg
      181 cggagctgct gccggacgtg gaggtctact acaaagcggt tcacgacgcc ttgacctatc
      241 aggagttctt cgattccaaa gagatcgatt ttgccgaaca agcgctcgcc acggcgaaag
      301 aacgcgccga ccagctttct gccggccagg ccccctggac gaacgccacc ggccgcgtcg
      361 tccgcggcta tgtctcgaaa atcgacggct cggtgcagcc gtacgggttg attgtgccgg
      421 aaagctatac gaccaagggt cccggccgct tccgttgcga tttgtggttc cacggccgcg
      481 gcgagacgct gagcgagctg aatttcgttc gcgaccggac gaacaacccg ggccagttca
      541 cgcccgaaga cacgatcgtc ctgcacccgt acggccgcta ctgcaacgcc ttcaagttcg
      601 ccggagaaat tgacgtgctc gaggcgctcg aagccgtcaa gcggcactac cgggtcgatg
      661 aagaccggat tggagagcgc gggttctcga tgggaggggc ggcggcgtgg caattcgccg
      721 tccactatgc cgaccgctgg tatgcggcca atccgggggc gggattcagc gagacgcccg
      781 aattcctccg catcttccag caggaagagc tgcagcccac ctggtgggag cagcgcctct
      841 ggcattggta cgactgcccc gactggtgcc tcaacctgcg gcattgccca ctcgtcgcct
      901 acagcggcga gctcgatacg caaaagcaag ccgccgacat catgcaagcc gcgctggccg
      961 aacaggggat cgacctgttg cacctcattg gcccgcaaac caggcacgcc atccacccgc
     1021 agtcgaaaat cgaaatcgaa agccggatgc agtccctggc ccgcgccggt cgcgactggc
     1081 agccgctcga tatcgagttc accacctata cgctccgcta caaccggatg cactggatcg
     1141 cgatcgatgg cctgcaagag cactggcagc gcgggcacgt ctcggcttcg ctgctggccg
     1201 gggaagtcgt gatcgactgc ggcggcgtga gcgacatgac gctctcgttt cccgccggcg
     1261 aatgcccgct ggacgtgcag tcgcccgtga ttgtcaccat cggcgagcag gaactggaag
     1321 ccccggcgcc caagagcgac cgctcgtgga acgtctcgct gcacaagatt cgcggcgagt
     1381 ggcggttggg aaaacggccc ggcaacgagc tccgcaaaca gccgggattg caaggcccga
     1441 tcgacgatgc cttcctcgat tcgtttctct tcgtgcgccc gacgggctcg gcgtggcatt
     1501 cgcccgtcgg caactgggca aagagcgaaa tggagcgagc cgtggagcat tggcggcggc
     1561 atttccgcgg catcgcgcgg gtgaaggacg acagggcggt cacgccggac gacattgccg
     1621 gctcgaatct cgtcttgtgg ggcgatccgg cgagcaattc gctgctcaag cagatcgccg
     1681 gcaaactgcc gatccgctgg accgagcagc aaatcgaggc gggcgacgaa aaattccccg
     1741 cgaccaatca cgcgctcatc gccgtttatc ccaacccgct caatccgcgg cggtacgtcg
     1801 ttctcaacag cggcttcacg ttccgcgact acgcctacct gaacaacgcc cgccaggtgc
     1861 cgatgctgcc cgactgggcc gtggtcgatc tgagcacgcc gccgggcagc gtctggccgg
     1921 gtaaaatcgc cgccgcggat ttcttcgacg aacgctggca gctcaaaacc agccccgagc
     1981 gcagcaaaca ttgactactg catcgggcac aaggggctcc ccgtgaactg ctagcgagag
     2041 atgcacggca agcctggctg cgggtaggag gcggtttcag tccagagctg cgaacttctt
     2101 catgcgaatt cttggcaatg cgcccctctc tcgctacaat ccgctcattc ccagcaggtc
     2161 cagcggtttc tcctcgttcg tcgtccgctt ggcaggaagc cactgctgct tccgcgtctt
     2221 ttctgagggg tgatgacgat catctagttt gaaaaaatca gcgggatcgc tgcgcccctg
     2281 atgtttgtga tgcattttcc acgccgcgaa ctactatcaa tagctcaaga ctgtgctccc
     2341 taaaccatgc gtgccggtgc tggcagaccc gcccggcaca tagcgttttg gctcggcctc
     2401 tctcctcagg aacagagaaa tgaagagctc catttgcagt gttatcgtc
//
LOCUS       DASUGB010000739         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_499626, whole genome shotgun sequence.
ACCESSION   DASUGB010000739 DASUGB010000000
VERSION     DASUGB010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499626"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFV87_14170"
     CDS             <1..1011
                     /locus_tag="VFV87_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104960.1"
                     /translation="VQLERAREILHRAAEHRERFQAALASADGGLGLRISDGVLADLR
                     FLDSIALGCRLEALAAAEALAENEPEAALPPLATMLRAARLVGAEWNVTTRVSAANLR
                     ADALDVLQAIAAHDYATRDTHEQLLEMLTLETADWPSDAQAWIGDRAAGLAVYELVRD
                     GYYLSLLSPEEMQRLQEQRLVPATAQAVHRNLDGDELFYLQAMRRIIASCEQPYYQRA
                     AVLNEIRGELAALEQTGDYPMVAGELLLGDFATGHRRQAEDLTRCTAVSTALLAATGQ
                     PLAPAPLSCLTGQPIDIERTPLGIQVLGWSLDRAPLEIPLRPLPRAARASLQDDEVTT
                     DK"
     gene            complement(998..2164)
                     /locus_tag="VFV87_14175"
     CDS             complement(998..2164)
                     /locus_tag="VFV87_14175"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104961.1"
                     /translation="MRPTFPIDLAATIAVTLAFTGLTSCDKVHEAVQTAQTEAAKLQE
                     KAETEAKKLQDQAMQQVEESSSAGSQTAAAGNPAAASEAAPASAGSAPAASTFQLPAA
                     RPGKSGAQVIAEFLALMPSERRDEHLLALAALDPSDRETIEILELQGSTVSSTGVAVL
                     PQFPKLHTLDLDLVRLTEQDAAVVGTLQGLKRLELSYTQITDVGLAHLASLVLLQSLD
                     LSGLQITGAGLESLKDLTELKQLSIPRTPLAGGELTVLRCLPKLKHLNVSHTMFAGGG
                     DKALRQLDDLETLIAVECGMDDRLLLQLKGANQLQVLRLSGNLVSDQSARTLSGFRAL
                     TELDLDSTQVGDLTLAALRRLDKLQTLNVGRTRCTPKGVADLQKVQPDLQITYQ"
BASE COUNT          522 a          901 c          869 g          519 t
ORIGIN      
        1 gtgcagctcg agcgggcgcg cgagatcctg catcgagcgg ctgagcatcg cgagcgcttt
       61 caagcggcat tggccagcgc cgatggcggc ctgggcctgc ggatcagcga cggcgtgctc
      121 gccgaccttc gcttcctcga ctcgatcgcg ctcggctgcc ggctggaagc gctggccgcg
      181 gcggaggcac tggccgagaa cgagccggag gcggccctgc cgccgctggc gacgatgctc
      241 cgcgccgcgc gcctcgtggg agccgaatgg aacgtcacga cgcgcgtctc ggccgccaat
      301 ctacgggccg atgcgctcga cgtcctgcag gccatcgccg cccacgatta cgccacgcgc
      361 gatacgcacg agcagctcct cgaaatgctg acccttgaaa cggcggattg gccttccgac
      421 gcacaggcct ggatcggcga ccgcgcagcg gggctggccg tctacgagct ggttcgcgac
      481 ggctactacc tctcgctcct tagtccagaa gagatgcaga ggctccaaga gcagcgtctc
      541 gtcccggcca ccgcccaagc cgtgcaccgg aatctcgacg gcgatgagct gttctacctg
      601 caggccatgc ggcggatcat cgcgagctgc gagcagccgt actaccagcg tgcggcggtc
      661 ctcaacgaaa tccgcggcga gctagcggcg ctggagcaga cgggcgatta cccaatggtt
      721 gccggcgagt tgctgctggg ggacttcgcg acggggcatc gccgccaggc cgaggatctg
      781 acccgctgca cggccgtcag caccgccttg ctcgccgcga caggccaacc actggcccca
      841 gcgccgctga gctgcctcac cggccagcca atcgacatcg agcgaacacc gctggggatt
      901 caggtccttg gctggtcttt agaccgggcg ccgctggaga tccctctgcg gccgttgccg
      961 cgagccgccc gcgcgagcct gcaggacgac gaagtgacta ctgataagtg atctgcagat
     1021 cgggctgcac cttctgcaaa tcggccactc ccttgggcgt gcagcgcgtc cggcccacgt
     1081 tgagcgtttg cagcttgtcc aggcgccgca acgccgccaa tgtcaggtcg ccgacttgcg
     1141 tgctgtccag atctagctcc gtcagggccc ggaacccact tagagttcgt gccgactggt
     1201 cggaaacgag gtttcccgaa agtcgcaaga cctgtaactg gttggcgccc ttcagttgca
     1261 gaagcaggcg gtcgtccatc ccgcattcta cggcgatgag cgtctccaaa tcgtcgagtt
     1321 gacgcagtgc tttgtctccg ccgccggcaa acatcgtatg cgagacgttc aagtgcttga
     1381 gttttggcaa acaacgcaga acggtcagtt cgccgccggc taagggggtt cgggggatgc
     1441 tgagctgctt cagctcggtc aggtccttga gcgactccag gccagccccg gtgatctgca
     1501 aaccgctcag gtccaaggat tggaggagga ccagagaggc caaatgtgcc aaccccacgt
     1561 cggtgatttg agtataggac aattccaggc gcttcaggcc ctggagtgtg cccaccaccg
     1621 ctgcatcttg ttcggtgagc ctcaccagat ccaaatcgag cgtgtgcagc ttgggaaact
     1681 ggggcagcac agcgactccg gtggacgata cggtgctgcc ctgcaattcc aggatttcga
     1741 tcgtttcccg gtcgctggga tccaaggccg ccagcgccag caagtgctcg tcgcgccgct
     1801 cagaaggcat cagtgccaag aattcggcaa tcacttgcgc cccgctcttt ccaggccgtg
     1861 ccgcggggag ctgaaacgtc gaagcagcag gcgccgatcc ggcgctggca ggagccgcct
     1921 cgctcgctgc agcgggattg cccgctgctg cggtctggct ccccgcgctg gacgactcct
     1981 caacttgttg catcgcctga tcctgcagct tcttcgcttc tgtctcggcc ttttcttgaa
     2041 gtttcgcagc ctcggtctgc gcggtttgga ccgcttcgtg cactttgtcg caggatgtca
     2101 ggccagtgaa cgccagggtc accgcgatcg tcgcggcaag atcgattgga aaggtcggac
     2161 gcatagggag actcgcagga agcgatgaca acgaacgtcg acagacgcaa atcctatgat
     2221 gagcgatcgg cccggcacgg ctcaagcgaa actctaccga tagcgatctc cgctccatgc
     2281 ttttgggggg ggcattgaac acatacctga ctttgcccag cattgccctc agacgtcagt
     2341 accgcgattc cgccattcgt gctagcgagt cggcacgagc tcaagccatc acgctgaatt
     2401 ccacgaaatt ccggaacaaa ctattctcaa acccagccgc accgtcgtag aatgagggac
     2461 gtacgaagga gatactggct gggcagtttc atgtgaacca cgttctgtgg tcctctccag
     2521 ccggtgactg gatttgtctc gtcaatcagt ttccacgcgg gtgagcaagt ggggcgggag
     2581 catctcgcct tgcgtccggg tttgcgcccc gacgtggcga cgttccaatc tcgcgaacga
     2641 attcccgcaa cggatcgaat tgcacgggca acctgccgaa cctccgcggg cggcggaaca
     2701 gggcggtccc aaatcggttc aatttcataa cggagttttg aacctttctt caaaggttca
     2761 gtaagaggca atcatggtga ccttgaccac gcgccccgca aacgccgaac t
//
LOCUS       DASUGB010000740         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_499838, whole genome shotgun sequence.
ACCESSION   DASUGB010000740 DASUGB010000000
VERSION     DASUGB010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499838"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1964
                     /locus_tag="VFV87_14180"
     CDS             279..1964
                     /locus_tag="VFV87_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5104962.1"
                     /translation="MACLATVSLLALAAVPAPAAPEESKAKSAGPTVPDYGLPQVARI
                     NQEIRQVWTDNNLQPSPPASDGEWCRRVYLDVLGRIPSVQELREFITSKEADKKSKLA
                     SKLLYDETYTEEYARNWTTTWTNILIGRSGGTERNTLTSREGMQKYLRDSFARNKPYD
                     RIVYELVTATGSTMPGTPDFNGATNYLAMKINEEKAAQATAMTSKIFLGLQVQCTQCH
                     NHPFNEWKQQKFWEFNAFFRQTQVLREFTPGTRDVTSATVTDSSWAGESNDASEAILF
                     YELRNGLLQSAFPVFVDGTELPKSGYLEDCNRRVELGKLIVASPFMDKTMANRMWQHF
                     LGYGFTKPIDDLGPHNSPSHPALLDYLGEQFRQNSFNVKELMRWIVLSEPYSLSSKVT
                     GQNKSDDPLLGESPKFTHFYLRQMQAEQLYESLLVATRAHENRGSYEEQEKLKSEWLQ
                     QFTLAFGTDEGDEATTFNGTIPQALMMMNGDLVQRAINKDQGSFVNVIAGSKLSPPEK
                     IEYLFEAAMSRKPSGGEIGVANKLLVARQGDTAGAMQDIFWVILNTNEFILQH"
BASE COUNT          488 a          677 c          642 g          400 t
ORIGIN      
        1 gccactctgg accaaccgat gccgggcatc cgtctccggg cccctgaagt tgtctgacgt
       61 atcgatgcgc aattttcgtt gcaccgggcg gaaggtcgcc tacaatggcg acctgaaatc
      121 gtggaaaagt ggtacgaact gagtacatct tcaacgtgca gcgcgtttcc atagttgtgc
      181 ccgtgcggcg gggctgtcct gaattggcga ggagcaaaac ggtgatgaat cggacggcag
      241 cgaaatctct gaaatccgtg agtgggttca acctggttat ggcctgcttg gccacagtct
      301 cgctgcttgc gctagcggcc gttcccgcgc ctgcggcgcc ggaagaatcc aaggcaaagt
      361 cagccgggcc gacggttccg gattacggcc tcccgcaagt ggcccgcatc aatcaggaaa
      421 tccgccaggt ttggaccgac aacaacctgc agccatcgcc gccagcgtct gacggcgaat
      481 ggtgccgacg ggtttatctc gacgtcctcg gccgcatccc ttcggtccag gaactgcgcg
      541 agttcatcac cagcaaggaa gcggacaaga aatcgaagct tgccagcaag ctgttgtacg
      601 acgaaaccta caccgaagag tacgcccgca attggaccac gacctggacg aacattctca
      661 ttggccgcag cggcggcacc gagcggaata ctctcaccag ccgcgagggg atgcagaagt
      721 atctccgcga ttcgttcgcc cgcaataagc cctatgatcg catagtctat gagttggtga
      781 cggcgacggg gagcacgatg ccgggcacgc ccgatttcaa tggggcgacg aactacctgg
      841 cgatgaagat caacgaggag aaggccgccc aggcgaccgc gatgacctcc aagatctttt
      901 tgggcctgca agtgcagtgc acgcagtgcc acaatcaccc gttcaacgag tggaagcagc
      961 agaaattctg ggaattcaat gcctttttcc gccagacgca ggtcttgcgg gaattcaccc
     1021 cgggcacgcg cgacgtgacc agcgcgacgg tcaccgactc gagctgggcc ggtgaatcca
     1081 acgacgccag cgaagccatc ttgttctacg agctccgcaa cggactgttg cagtcggcgt
     1141 ttccggtttt cgtcgatggc acggagctgc cgaagagcgg ctacctggaa gattgcaacc
     1201 gccgcgtcga gttgggcaag ctgatcgtgg cgtcgccctt tatggacaaa acgatggcca
     1261 accggatgtg gcagcacttt ttgggctacg gcttcacaaa accgatcgac gacctggggc
     1321 cccacaattc gccgtcgcac ccggcgttgc tcgactacct gggcgagcag ttccgccaga
     1381 acagcttcaa tgtgaaggag ctgatgcgct ggattgtgct gagcgagccc tattcgctct
     1441 ccagcaaggt caccggccag aataagtctg acgatccctt gctcggcgaa tcgcccaagt
     1501 tcacccactt ctacctgcga cagatgcagg ccgaacagct ctacgaatcg ttgctcgtgg
     1561 ccacgcgggc ccacgagaat cgcgggagct acgaagaaca agaaaagctg aagagcgagt
     1621 ggctgcagca gttcacgctg gccttcggca ccgatgaggg ggacgaagcg acgaccttca
     1681 acggcacgat tcctcaggcc ctgatgatga tgaacggcga cctggtgcag cgggccatca
     1741 acaaggatca aggcagcttc gtcaatgtca tcgccggcag caagttgagc ccgcccgaga
     1801 agatcgaata cttgttcgaa gcggccatgt cccgcaagcc gtcaggcggc gagatcggtg
     1861 tcgccaacaa gctgctggtc gcccgccagg gcgatacggc cggcgcgatg caagacatct
     1921 tctgggtcat cctgaacacc aacgagttta ttttgcagca ctaaatggag acattggcat
     1981 atcgtcgggc gtttatgtat cctgacgcct gcctgctccc cccccccaat aaaatcagcc
     2041 tcactaaact ttcggagact ccctcaatga agttcgctac tcccgccggc atgacgcgcc
     2101 gccatttcat ggcccacatg gccggcagca gcgccgccgc cacagccgcc ctcagcttcg
     2161 gccacagcct gcgggcccac gctgccgacc tgaagcggaa tcacaag
//
LOCUS       DASUGB010000741         6324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1887551, whole genome shotgun sequence.
ACCESSION   DASUGB010000741 DASUGB010000000
VERSION     DASUGB010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887551"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFV87_14185"
     CDS             complement(<1..737)
                     /locus_tag="VFV87_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104963.1"
                     /translation="MAKRSRARVEDDLDDDYYEDEEFEERPARRKRREPGPRQGSFLG
                     WFASRLLVVVVLLAVLVFFAPWIVSATGLWKPILAWAAPDVAGKVEIGTLRLGWISGI
                     DLGDVTIRDAAGQPLAVAGRITSRKTLLEIVQNQEQLGTFDIQGPKINLVLREDGSNL
                     EDFLASLPKSSDEPSAVGFAVTVTSGSIQLDDQISSRQWQLDNVNFDFAWPAAASEAK
                     TGKLSAALKPAGEAAAPATAGEIAAEFS"
     gene            1041..1691
                     /locus_tag="VFV87_14190"
     CDS             1041..1691
                     /locus_tag="VFV87_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104964.1"
                     /translation="MDPLHVCIALGPVAVYLLVLGTINLSPRPFLTSGGRDAAALGIA
                     IAGFAVAGPMELFLPVEAAAYWGPWVWPMMLGAYVLGLLLVILMLRPRLVVYNATAEQ
                     LLPILDAVAKRLDPAATWAGESLTLPSLAVELHVESWPVLKNVQLVSAGPLQNFAGWR
                     QLELELAQALRSVHSTRNPAGFSLLTVGLAIALAITYWLSRDPTGVQQALNQMLRH"
     gene            1864..2967
                     /locus_tag="VFV87_14195"
     CDS             1864..2967
                     /locus_tag="VFV87_14195"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5104965.1"
                     /translation="MSSPTTLLPPQTSPLPAGGPHLRHDAAHVQAAPIGEYRHVISRL
                     TGVQVLGSGAFVPPQIVTNADLAALGYDADWIVQRSGILERRLAADDVACSDLAYEAA
                     RRCLDQAGVEADQLDLIVVATLTGDSPIPSTACQLQRRLGGRAAAFDVGAACSGFMYA
                     LVTGMQFVKTGSARRVLVVGSEIMSRIINPADTKTFPLFGDGAGAVLLGAGNEEQGLL
                     AYTLGADGSGADLLQIPGGGSREPLSAERIAGGRQYIQMEGRTVFKWAVNLLADSVRQ
                     VLAEARITTDDLSHFILHQANGRIVDSAAKHLGIRPEKVVLNIDRYGNTSAASIPLAL
                     DEIHRAGRLARGDYLLLSGFGAGLSWGTAVLRW"
     gene            complement(3025..3522)
                     /locus_tag="VFV87_14200"
     CDS             complement(3025..3522)
                     /locus_tag="VFV87_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104966.1"
                     /translation="MKRMTACVAVIACWALSSAALADYGVENRGTWPASWPKELEPLR
                     GQARTLEGPLVLLLHYAIPFTERAQFEAAWPNLVKVKSPGAPILLRRAPNFFLGGKNQ
                     AGVCIHAPPEGEQPLSAKESNGNWQKTIYLELIVDGDIVDLNRIRLPPDTPIIDERFQ
                     PAANQ"
     gene            3975..4739
                     /locus_tag="VFV87_14205"
     CDS             3975..4739
                     /locus_tag="VFV87_14205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5104967.1"
                     /translation="MLPLYVASLNSYVSSALRSARRLTGREPFEAVKRYRLAETAMDR
                     IPDGINDQEAMRAFERLRRELAQAEERLSRILKHDRLIGAQAAPLQVEHWVSGAPLTD
                     RDLKGKVVILDFFAVWCRPSIETFPELAKWREKHGEQGLMIVGLTNYYNYHWDERTQG
                     PRQTSGEVPQQTEHEMLQRFAKQHELKHSLGTLTGEDIHNFYGVTAIPQIVVVDRQGV
                     IRYIGAGNQSENIPEITALLDKLLGDPKLRDATNPR"
     gene            4977..>6324
                     /locus_tag="VFV87_14210"
     CDS             4977..>6324
                     /locus_tag="VFV87_14210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5104968.1"
                     /translation="MAVVLRLPPRANVKLEDTWDLSPMCENDASWEREFQKLDKMIAG
                     FEKFRGKLGESAKTLAACLKFDCDFDRLGERIGGYAQLKLTEDQTNSTYQGMVAKFTN
                     LATRASQAASYIRPEILAIPKKTLDQYLNAKEMQPFKLVLERIIRFRPYTLSPREEEI
                     IAMQGEMAGGVGKAFRQLLDADLKWGLVKNEKGEQVELSNATFSKLLVSPERKVRKTA
                     FHQYYTQFKSHENTLAATLAGSIHKDVYYAKVRGYKASLDAALFPDNVPHAVYDNLIA
                     AVRKHLPAVHRYYDVRRRKMRLKDIHHYDTYVPILSDLEIKRNWNQAVKLVMDALQPL
                     GEEYCRTLQAGLTSARWCDRYPNQGKSSGAFSSGSYDSPPYILMNYNPDVFNDVFTLA
                     HEAGHSMHSWHSAKHQPYQYYGYVIFVAEVASTFNEELLSHHLLEHARDNRQRAYLI"
BASE COUNT         1203 a         1988 c         1952 g         1181 t
ORIGIN      
        1 gagaattctg cggcgatctc gccagccgtg gctggcgcgg cggcttctcc cgcgggcttc
       61 agcgcggcag agagcttgcc ggtcttggct tccgaagcgg cggctggcca ggcgaagtcg
      121 aaattgacgt tgtcaagctg ccactggcgg ctggagatct gatcgtcgag ctggatcgag
      181 ccggacgtga ccgtgaccgc aaagccgaca gccgatggct catccgacga cttgggcagc
      241 gacgccagaa aatcttccag gttgctgccg tcctcgcgga ggacgaggtt gatcttcggg
      301 ccttggatat cgaacgtccc cagctgctct tgattctgca cgatttccag cagcgtcttg
      361 cggctggtga tgcgacctgc cacggcgagc ggctggccgg cggcgtcgcg gatcgtcacg
      421 tcgcccagat cgatgcccga aatccacccc agccgcagcg tgccaatctc gaccttgccg
      481 gccacgtccg gcgcggccca cgcgaggatc ggcttccaga gtccggtcgc actgacgatc
      541 cacggcgcga agaagaccag caccgccagc agcacgacga cgacgagcaa gcgcgacgcg
      601 aaccagccca agaaggatcc ctgccgcggc cccggctcgc ggcgcttgcg ccgcgccggc
      661 cgctcctcga attcctcgtc ttcgtaatag tcgtcgtcca ggtcgtcctc gacccgcgcg
      721 cgcgatcgct tcgccatggc gaatcccttc cgagtgcttt cggttcaccg caaccatctc
      781 gctccgcgag atgtgatcga ctccgccatg cgcgaaggcc ctcgcgcatg gaaaaccacc
      841 cgcgactcgc ggagcgagtc gcctacggtg tgtcgcggga ttctaggcaa tttgcgcttg
      901 tagggcctag agcgaactcc cgctcgcgca aatcttgtcg ctgtaacgac ttgcaccatc
      961 gcgttgaccg ctattgccgg gcggccttag aatcggggga accgtcgccg acgcacttta
     1021 aattgacctg aaagcacctc atggacccgc tgcacgtttg catcgcattg ggaccggtgg
     1081 cggtgtattt gctggtcctg gggacgatca atctctcgcc gcggccgttt cttacgtccg
     1141 gtggccggga tgccgcagcc ctgggaatcg cgattgcggg gtttgcggtc gccgggccga
     1201 tggagctctt cctcccggtc gaagcggccg cttattgggg cccgtgggtc tggccgatga
     1261 tgctcggagc gtatgtgctg ggcctcttgc tcgtcattct gatgctccgc ccgcggctgg
     1321 tggtctataa cgcaacggcc gagcaactgc tgccgatcct cgacgcggtg gccaaacggc
     1381 tcgaccccgc ggcgacctgg gcgggcgaaa gcctgacgct gcccagcctg gccgtggaac
     1441 tgcacgtcga gtcgtggccg gtgctgaaaa acgtgcagct cgtcagtgcc gggccgctgc
     1501 agaacttcgc cggctggcgg cagcttgaat tggaactggc ccaagcgctc cgctctgtcc
     1561 acagcacgcg caatccagcc ggcttcagcc tgctcaccgt agggctggcg atcgccctgg
     1621 ccatcaccta ctggctctcg cgcgatccga cgggcgtcca gcaagcgctg aaccagatgc
     1681 tgcggcacta gcaatggggt cgcaagtcct tttgctgtaa cgccttgcgc tagcggatac
     1741 cgttctggca tcgctccacg gcctcactat accgaattcc gcgaaatcgg tataaatcac
     1801 caacttgtct gggcgaaagg cgtctgtgcc cctccgaaag caagcaagat cagcacaccg
     1861 cgcatgtcct ctccgaccac cttgctgccc ccacaaacct ccccgctccc agcaggcggc
     1921 ccgcacctgc gccacgatgc ggcacacgtt caggcggcgc cgatcggcga gtaccggcac
     1981 gtcatttcgc ggctgaccgg cgtgcaggtc ctcggcagcg gcgcttttgt gccgccgcaa
     2041 atcgttacga atgccgacct agccgcgctc gggtacgacg ccgactggat cgtgcagcga
     2101 tcgggaatcc tggagcgccg gctggcggca gacgacgtcg cctgtagcga cctggcttac
     2161 gaagcagccc ggcgatgtct cgatcaagcg ggcgtggaag ccgatcagct cgacctgatc
     2221 gtcgttgcca cgctgacggg cgacagcccg atcccctcga cggcctgcca actgcaaaga
     2281 cggctgggcg gccgggcggc agccttcgac gtcggcgcgg cctgctcggg cttcatgtac
     2341 gctctggtca ccggcatgca gttcgtcaag accggcagcg ctcgccgcgt gctggttgtc
     2401 ggctcggaga tcatgtcgcg gatcatcaat ccggccgata cgaagacgtt tcccctgttt
     2461 ggcgacggcg ccggcgccgt cttgctgggc gctgggaatg aagagcaagg gctcttggcc
     2521 tacacgctcg gcgccgatgg cagcggggcc gatttgctgc agattcctgg cggcggttcg
     2581 cgcgagccgc tctcggcgga gagaatcgcc ggcggccggc agtatatcca gatggaaggc
     2641 cgcaccgtct ttaagtgggc agtcaacctc ttggccgatt cagtgcggca agtactggcc
     2701 gaagcccgca tcacgacgga cgatctgtcg cacttcatcc tgcaccaggc caacggccgc
     2761 atcgtggatt ccgcagccaa gcacctcggc attcgtcccg aaaaagtggt gctgaacatc
     2821 gaccgctacg gcaacacctc cgccgcctcg attccgctgg ccctcgacga gatccaccgc
     2881 gctggccgcc tagcgcgcgg cgactatctc cttctctccg gcttcggcgc gggtctctcc
     2941 tggggaaccg ccgtgctgcg gtggtaagca gggcaaatct tgctcttgct ctgagccttg
     3001 gatgcggtcg caccgcacct ggttctactg attcgccgca ggctgaaacc gctcgtcgat
     3061 gattggcgtg tcgggcggca accgaatgcg gttcagatcg acgatatcgc catcgacgat
     3121 cagttcaaga tagatcgtct tttgccaatt gccattcgat tccttggcgc tcagcggctg
     3181 ctcgccctcg ggaggcgcgt ggatgcaaac acctgcctgg ttttttccgc ccaggaagaa
     3241 atttggcgcg cggcgcagca ggatcggagc gcccgggctc ttcaccttga ccaggttcgg
     3301 ccaggcggct tcgaactgcg cccgctcggt aaagggaatc gcatagtgca atagcaggac
     3361 gagcggcccc tccagcgttc gcgcctgccc tcgcagcggc tccagttcct ttggccagct
     3421 cgctggccac gtacccctat tctcgacccc atagtccgcc agggcggcgg aactcagtgc
     3481 ccagcaagca attacagcca cgcaagcggt cattcgtttc atcgattccc cttctagcag
     3541 gcggcgacag aatgctccgg tagaaagacg aataactgcc aacgctggtt ccgaactttt
     3601 ggagttctag gctggtgttt cagaagtttt ccgcggcagc ggcttgccgc taaagacata
     3661 atgtcttatt ctgggctcca tcgctttccc cttggatggc gcaagagcca aaccacgctt
     3721 tcccaactgc ctatacgatc tcgagacacg cttcgtggga gaaacattcc atgtggagag
     3781 ccgtattgac tgtctcgttg atttgtggcc tgttttccac cgcggctgcc caaaatccga
     3841 ccgatcggcc agcgcccgat ggcgatgcgc gaaggactga gtcgcccgag agacgggacg
     3901 gcgccagtcc cgaggcgcta caacagctta ctgagctgga gcagagactc gccgagaagc
     3961 cggacgacgc gaaggtgctc ccgctctacg tggcttcgct caacagctac gtctcctccg
     4021 cccttcggtc ggcacggcgg ctgacaggca gggagccttt cgaggccgtg aagaggtacc
     4081 gcctcgccga aacggcgatg gaccgcatcc cagacgggat caacgaccaa gaggccatgc
     4141 gtgcgttcga gcgcctgcgg cgagagcttg ctcaggccga ggagcggttg agtcggattc
     4201 tcaaacacga ccgattgatt ggcgcacagg cagcgccgct ccaggtcgaa cattgggtga
     4261 gtggagctcc gctgaccgat cgtgacctga aaggcaaagt ggtgatactc gacttttttg
     4321 ccgtctggtg ccggccgtcc atcgaaacct tcccagagct ggccaagtgg cgtgaaaagc
     4381 atggcgagca gggtctgatg atcgttggcc tgacgaacta ctacaactat cactgggacg
     4441 agcggaccca aggaccccgc cagactagcg gcgaggtgcc tcaacaaacg gagcacgaga
     4501 tgctccagag atttgccaaa cagcacgagc tgaagcacag cttagggacg ttgacaggcg
     4561 aagatatcca caacttttac ggtgtgacgg ccattcccca aatcgtggtc gtcgatcggc
     4621 agggcgtcat ccgctatatc ggcgcgggca accagtccga gaacatcccg gagattaccg
     4681 cattgctgga caagttgctc ggcgatccga agctacgcga cgcgacgaat ccgcgttaga
     4741 gaatccacgg aaggccctcg attcgtcttc ccgattcagc gcaactggtt gtcgcactga
     4801 gacttgcagt gcaagtgtgc ggcgggcgcg ttggaatgaa tgtcagatgt tccgcactgc
     4861 gcggcgaaac cctatcccca gcgcagggcg gtccccctct cgcttgctgg ctatcaggat
     4921 ttctaagatg atcgccagtt acgactgatt cactcacttg gacacggaat tgtcatatgg
     4981 ctgttgtatt gcgtttgccg ccgcgggcca acgtcaagct cgaggatacc tgggatctct
     5041 cgccgatgtg cgagaacgat gcgtcttggg agcgggagtt tcagaagctg gacaagatga
     5101 tcgccggctt cgagaagttt cgcggcaaat tgggggaaag tgccaagact ttggcggcgt
     5161 gtctgaagtt cgattgtgac ttcgaccggt tgggggagcg gattggcggc tacgcgcagc
     5221 tcaagctgac ggaagaccag accaacagca cgtatcaggg gatggtggca aagtttacaa
     5281 acctggctac gcgggcctcg caggcggcca gctacatacg accggagatc ctggcgattc
     5341 cgaagaagac gctggatcaa tacctgaacg ccaaggaaat gcagccgttc aagctggttc
     5401 tcgagcggat catccgcttt cggccttaca cgctttcgcc ccgcgaggaa gagatcatcg
     5461 cgatgcaggg agagatggcc ggcggtgtgg gaaaggcgtt ccgtcagctt ttggacgcgg
     5521 acctgaagtg gggcctggtg aagaacgaga aaggggagca ggtcgagctg tccaacgcca
     5581 cgttttcgaa gctgctcgtc tcgcccgagc ggaaggtgcg gaagaccgcg ttccaccagt
     5641 actacacgca gttcaagtcg cacgagaaca ccctggccgc cacgctggcc ggctcgattc
     5701 acaaggacgt gtattacgcg aaggtccgcg gctacaaggc gtcgctggat gcggctttgt
     5761 ttcccgacaa cgtgccgcac gcagtctatg acaatctgat cgccgcggtc cgcaagcatc
     5821 tgcccgcggt gcaccggtac tacgacgtgc ggcggcggaa gatgaggctg aaggacatcc
     5881 accactacga tacgtatgtg ccgatcctca gcgatctgga gatcaagcgc aactggaacc
     5941 aggcggtgaa gctcgtgatg gacgccctgc agccgctggg cgaggagtat tgccgcacgc
     6001 tccaggcggg gctgacctcc gcccgctggt gcgaccgcta tccaaaccag ggaaagagta
     6061 gcggagcgtt ttccagcggc agctacgaca gcccgccata catcctgatg aactacaacc
     6121 cggacgtgtt caacgacgtg ttcacgctgg ctcacgaagc ggggcactca atgcactcgt
     6181 ggcactcggc caaacatcag ccgtaccagt actatggcta cgtgatcttc gtcgccgaag
     6241 tggccagcac gttcaacgag gagctgctca gccaccacct gctggagcac gcccgagaca
     6301 accggcagcg ggcgtatctg atca
//
LOCUS       DASUGB010000742         7200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1611504, whole genome shotgun sequence.
ACCESSION   DASUGB010000742 DASUGB010000000
VERSION     DASUGB010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611504"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VFV87_14215"
     CDS             <1..1102
                     /locus_tag="VFV87_14215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5104969.1"
                     /translation="VLQLYGESEVSKWFRREGPQHRVILTQPVFVSVTEVTQSHFEQV
                     MGTTPSYFSATGAGKEAVANLETDNHPVETVSWNDAAEFCAKLSQKEKLKPFYFRSSE
                     TVTPLDGTGYRLPTEAEWESACRAGTTTRFWAGDEEQDLISADWYGRNSSGRTHAAGE
                     LKANPFGLSDMHGNVWEWGQDSWDPSFDGKIEQNVVVDPSGPFSAGSKRVFRGGNWLS
                     ESPLCRSSRRWANQPSYRDYVTGFRVVLPVGAVKGSLSAPPAATTSKPHQWPADAPPP
                     AIAPFDAEQAQAHQAAWAKYLGVPVEYTNSIGMKFRLIPPGEFLMGSTPEAIEAALKS
                     FVENCTLWRDLSVRRSDEYTTVLIQPNSNFRH"
     gene            1472..1645
                     /locus_tag="VFV87_14220"
     CDS             1472..1645
                     /locus_tag="VFV87_14220"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5104970.1"
                     /translation="MSDVTQILAQIESGDSSAADKLLPLVYHELRTLVAAMPVQEKPG
                     QMFYAQPTHNARH"
     gene            1772..2509
                     /locus_tag="VFV87_14225"
     CDS             1772..2509
                     /locus_tag="VFV87_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5104971.1"
                     /translation="MTTLAAAPAESRTVVFEARGLTKVYRMGETEVQALRGIDLDLYQ
                     GEFLVLLGPSGSGKSTLLNILGGLDVPTAGHVRFRDHDLSREDDELLTHYRREHVGFV
                     FQFYNLIPSLTAWENVALVTDVAERPMDPSEALARVGLQERRDHYPSQLSGGEQQRVA
                     IARAIAKQPDVLLCDEPTGALDVKTGVVVLEVIQRINRELGTTTAIITHNAAIAGMAD
                     RVVTLSDGRIAQIRTNDKKISAREIVW"
     gene            2513..4876
                     /locus_tag="VFV87_14230"
     CDS             2513..4876
                     /locus_tag="VFV87_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5104972.1"
                     /translation="MRTINIKLLRELWQMRWQALAIALVMACGVATAVMSLSTLDSLE
                     RTREAYFDRYRFADVFAHVKRAPRSLVARIEKLPGVEAVEARIVSGVTLDVAGLSEPA
                     TGRLISVPEFGGGGLNALHIRQGRAIDPQRSGEVLVAEAFAVAHGLVPGDRIRAVING
                     RLKELQIVGIALSPEYIYSARAGELLPDDKRFGVLWMGEDDLEAAFDLDGAFNDVSLS
                     LTPDASEAEVIRLLDRLLDPYGGTGAHGRADQPSYRFLENEMMQLRAMASLPPAIFLS
                     VTAFLLHVVLARLIATQREQIATMRAFGYSRWEIGRHYLGFALAITTVGTAVGLALGA
                     RFGSDLTVLYARFFRFPEFHYVLDWRIALLAGGVSAASAVLGVWSSVWRAVSEAPAQA
                     MQPEPPARYRASFLERWGTARLLSPETRMVIRHLERQPLRAALSCLGVALAIGILVLG
                     NFVEDTVDHVMNFQFERVQRQDVTVTFVEPVTGRARHDLAHLPGVIAVEPFRAVPVRV
                     RFGPVTRRLGVLGLLDGRRLFRPLDAQERQIELPAEGVVISQKLAEVLGCGLGDRLQL
                     EVLEGERPVRDVPIAQIVSDFIDLNVYMRLDALQRLMREQDAISGAFLAVDPASADVL
                     YAELKRTPRIAGVTIKSAALESYLQTLAENVLRMKAINVIFAAVVAFGVVFNAARITL
                     AERSHELATLRVLGFTQRETARILFGELVVIVVVAIPVGWLCGYAFAGFLTASLSTEV
                     HRFPLLVSSGTYAFATLVVVVAVVVSLLVVQRRLSRLELVSVLKARD"
     gene            4877..6085
                     /locus_tag="VFV87_14235"
     CDS             4877..6085
                     /locus_tag="VFV87_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5104973.1"
                     /translation="MKKRRIDWLWVLAAIAVAAGAAYVLWPRPVEVDLAEVTRGPLVV
                     TVDEDGKTRVKERFIVTAPLAGQMLRIDLEEGDPVETGKTLLASIEPVDPALLDARTR
                     SEAEARVRSAEAAREQATAGLEEAREAHEIARHEFERVAALVKTKAVTESEYDRAEHE
                     ERLRAQSLRSAEFALRVAEFELEIAKAAFIRTQPDQDQDASQRMELRSPITGRVLRVL
                     QESATVVTPGLPLLELGDAQDMEVEVDVLSSDAARIRPGARVFLEHWGGDKPLAGRVR
                     LVEPSGFTKISSLGVEEQRVNVIADFVSPLSERQGLGDAYRVEARIVVWETLEATLVP
                     AGCLFRRGNEWAVYVVENGRARLNTVQVGHSNGVQTEVLSGLAPEAQVVLHPGDKVVE
                     GTSIQRRSSS"
     gene            complement(6131..6295)
                     /locus_tag="VFV87_14240"
     CDS             complement(6131..6295)
                     /locus_tag="VFV87_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104974.1"
                     /translation="MRQFQHLLGVSHRVANRPNRFEPRLKKQRRNHDGWLTQPRAEVK
                     RDMAKGVINI"
     gene            6839..>7200
                     /locus_tag="VFV87_14245"
     CDS             6839..>7200
                     /locus_tag="VFV87_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEX5104975.1"
                     /translation="MSISLEKVAEKYLVAKKLSTGTQKEYRSTVTKWLAWGQGVDVDQ
                     IDRRHIRDYLDWVHDKATNDGGSNAGRTANKARENLRAILSWAWEQDYVEKLPRFPKP
                     KPQRDVAGRHYLTKSDLNA"
BASE COUNT         1310 a         2094 c         2429 g         1367 t
ORIGIN      
        1 agttttgcag ttgtacggag aaagcgaggt gagcaaatgg tttcggaggg aagggccgca
       61 gcacagggtc atcctcacgc aaccagtctt tgtcagcgtg accgaggtga cacaatcgca
      121 tttcgagcag gtgatgggga ccaccccgtc gtacttctcg gccacgggtg cagggaagga
      181 agcggttgcc aacctggaga ccgacaacca tccggtggag acggtgagct ggaacgacgc
      241 ggccgagttc tgtgcgaaac tgagccagaa ggaaaaactg aaaccgttct acttccgatc
      301 ctctgaaacg gtgacgccgc tcgacgggac gggttaccga ctcccgacag aagcggaatg
      361 ggagtccgct tgccgggcgg gcaccacgac gcgcttctgg gccggggatg aagagcagga
      421 cctgatttca gccgactggt atggccgcaa ctccagcgga cgcactcatg ccgcaggtga
      481 gctgaaagcc aatccgttcg gactgtcgga catgcatggc aacgtgtggg aatgggggca
      541 ggacagttgg gatccgtcat ttgatggcaa gatcgaacag aacgttgtag ttgacccgtc
      601 cggaccattt tccgccggct ccaaacgggt gttccgcggc ggcaattggc tctcggagtc
      661 gccgttgtgc cgttcgtcgc gtcgctgggc aaatcagccg tcttaccgtg actacgttac
      721 cggtttccgt gtggtgttgc cggtgggtgc ggtgaaggga tcgctgagcg ccccgccggc
      781 ggcaacaact tccaagcccc atcaatggcc cgccgacgct ccgccgccgg cgattgcgcc
      841 gttcgatgcc gagcaggcgc aggcccatca ggccgcgtgg gcgaagtacc tcggcgtgcc
      901 ggtcgagtat accaacagca tcggcatgaa attccgcctc atcccgccgg gcgagttcct
      961 gatggggagc acgccggagg cgatcgaagc ggcgctgaaa tcctttgttg agaactgcac
     1021 actctggcga gacctcagcg tccggcgatc ggacgagtat accaccgtgt tgatccagcc
     1081 gaattccaac ttcagacact gacttccgcg catgcacagt ctgcgacaga atctgcgtaa
     1141 cgtcggtcat ttcgaccagg atactgccga gaaacagtag ttgctattgg ctggcccagt
     1201 cgcaaacgta ggcgcggagg tgaagaagac cgccagcagt ttcgcccaat acaaactcgc
     1261 agcggttgca tcggccacgg gcgccacgaa gatcttgcca gctgcagcgg tgtctcttcg
     1321 cgcaggacac cgctgcgagt ttccgccggc cgtaactcac ggttgttggt ccaaccacca
     1381 gcacgaagag tggtcacgaa ttgcaggagg tcaaagggac cgatgcggct ttcgattgcg
     1441 agatgatggt ggcccgataa caatgagggc catgtccgac gtgacccaaa tcctggcgca
     1501 gattgagtcg ggcgattcat cagccgcgga caagctcttg ccgctcgttt accacgaact
     1561 gcggacgctt gtcgctgcaa tgccggtgca ggagaagcca gggcaaatgt tctatgcaca
     1621 acctacgcac aacgctcgtc attgacgtct agcagcattc ccacgtacct cagaatggat
     1681 tcgccacaat ggtagctgca accccgatag tctgtctgaa ttcaatcgct gagccggtca
     1741 aagagcctga tcactttcgc cagtggcatc gatgacgacc ctagccgccg cacccgctga
     1801 gagccgcacc gtcgtctttg aggcccgcgg cctgacgaag gtctatcgca tgggcgagac
     1861 ggaagtgcag gccctgcggg ggatcgacct ggacctgtac cagggggagt tcctggtgct
     1921 gctcgggcca tcgggcagtg ggaaatcgac gctgctgaat atcctcggcg ggctggatgt
     1981 gcccacggcg gggcacgtgc ggttccgcga tcacgacctg agccgcgagg atgatgagct
     2041 gctcacgcac tatcgccgcg agcacgtggg cttcgtgttt cagttctata acctgatccc
     2101 cagcctgacc gcgtgggaga acgtcgcact ggtcaccgac gtcgccgagc ggccgatgga
     2161 tccgagcgag gccctggccc gcgtcggcct gcaggagcgg cgcgaccact atccctcgca
     2221 gctcagcggc ggcgagcagc agcgggtggc gattgcccgg gcgatcgcca agcagcccga
     2281 cgtgctcctt tgcgatgagc cgactggggc gctcgatgtg aagacgggcg tggtggtgct
     2341 ggaggtgatt cagcggatta accgcgagct gggcacgacg acggcaatca tcacgcacaa
     2401 cgcggcgatc gccggcatgg cagaccgggt ggtgacgctg tcggacggcc ggatcgccca
     2461 gattcgcacc aacgacaaaa agatctcggc ccgcgaaatc gtctggtgag ccatgcggac
     2521 gatcaacatt aagctgctgc gggaactgtg gcaaatgcgc tggcaggcgc tggcgattgc
     2581 cctggtgatg gcctgcggcg tggcgactgc cgtgatgtcg ctgagcacgc tcgattcgct
     2641 ggagcggaca cgggaggcct atttcgaccg ctaccgcttc gccgacgttt ttgcccacgt
     2701 caagcgagcc ccgcgctcgc tggtggcgcg aatcgagaag ttgcccggcg tggaagccgt
     2761 tgaagcgcgg atcgtttccg gcgtgacgct cgacgtggcc gggctctccg agccggcgac
     2821 cggccgcctg atttcggtgc cggagtttgg gggcggcggg ctcaacgcct tgcacatccg
     2881 gcaaggacgg gccatcgatc cgcagcgttc cggcgaggtg ctggtggcgg aggcattcgc
     2941 ggtggcccat ggcttggtgc ccggcgaccg gatccgggcg gtgatcaacg gccggctgaa
     3001 ggagctgcaa attgtgggga tcgcgctgtc gcccgaatat atttactccg cgcgggccgg
     3061 cgagctgctg cccgacgaca agcgattcgg cgtattgtgg atgggcgaag atgatttgga
     3121 agcggccttt gatttggacg gggcgtttaa cgatgtttct ctctcgctga cgccggacgc
     3181 ctcggaggcg gaagtgattc ggctgctcga ccgtttgctc gatccgtatg gcggcacggg
     3241 cgcgcatggc cgggcggacc agccgtcgta tcggtttctg gaaaatgaga tgatgcaact
     3301 gcgggcgatg gcctcgctgc cgccggcgat cttcctctca gtgacggcct tcctgctgca
     3361 cgtggtgctg gcccggctga tcgccacgca gcgcgagcag atcgcgacga tgcgggcgtt
     3421 tggttactca cggtgggaaa tcggacgaca ttacctggga ttcgcgctgg cgattaccac
     3481 cgtcggcacg gcggtgggac tggcgctcgg cgcgcgtttc ggcagcgacc tgacggtcct
     3541 ctacgcgagg ttcttccgct tccctgagtt tcactatgtt ctcgactggc ggattgcgct
     3601 gctcgcgggc ggggtcagtg cggccagtgc cgtgctgggc gtctggtcgt cggtgtggcg
     3661 ggcggtgagc gaagccccgg cccaggcgat gcagcccgag ccgccggccc gctaccgcgc
     3721 gagttttctg gagcgatggg gcacggcccg gctgctgtcg cccgagacgc ggatggtgat
     3781 ccggcatctg gagcgccagc cgctgcgggc ggcgctgtcg tgcctgggcg tcgcgctggc
     3841 aattgggatt ctcgtgctgg gcaattttgt cgaagacacc gtcgatcacg tgatgaactt
     3901 tcagtttgag cgcgtgcagc ggcaggacgt gacggtaacg ttcgtcgagc ccgtcaccgg
     3961 ccgcgcgcgg cacgatctgg cgcatctgcc gggagtgatc gcggttgaac cctttcgagc
     4021 cgtgccggtc cgggtgcggt ttgggccggt aacgcgccgc ttgggcgtgc tggggcttct
     4081 tgatggccgc cgactcttcc ggccgctcga tgcccaggag cgacagatcg aactgccagc
     4141 cgagggggtc gtcatttcgc agaagctggc cgaagtgctc ggctgcgggt tgggagatcg
     4201 gctgcaactg gaggtcctgg agggagaacg ccccgtccgt gacgtgccga ttgcgcagat
     4261 tgtcagcgac ttcatcgatt tgaatgttta catgcggcta gacgcgctgc agcgcctgat
     4321 gcgcgagcag gatgccattt cgggggcctt tttggcggtc gatccggcca gcgcggacgt
     4381 gctttatgct gagctgaagc gcacgccgcg gattgccggt gtgaccatca agagtgccgc
     4441 gctggagagt tacctccaga cgctggccga aaacgtgctc cggatgaagg cgatcaacgt
     4501 gatttttgcc gcggtcgtag cctttggtgt ggttttcaat gccgcccgga tcacgctggc
     4561 agagcgtagc cacgaattgg ccacattgcg ggtgcttggg ttcacgcagc gcgagaccgc
     4621 ccgaatcctg ttcggcgagc tggtagtgat tgtcgtggtc gcgattcccg tcggctggct
     4681 ctgcggctat gctttcgcgg ggttcctgac ggcttcgctc agcaccgaag tgcatcgctt
     4741 tccgctcctc gtcagcagcg gaacgtatgc ctttgcgacg cttgttgttg tggtggccgt
     4801 ggtggtctcg ctgctcgtcg ttcagcggcg gctgtcgcgg ttggaactgg tttccgtgct
     4861 caaggcccgc gattagatga agaaacgccg catcgactgg ctgtgggtac tggctgccat
     4921 cgcagtggca gcgggagcgg cttatgtgct ctggccgcgg ccggtcgagg tcgatctggc
     4981 ggaggtgacg cgcggcccgc tggtggtgac ggtcgacgag gatggcaaga cgcgggtcaa
     5041 agagcgattt attgtcacgg cgcccctggc cgggcaaatg ctgcgaattg atctggagga
     5101 aggcgatccc gtggagacag gcaagacgct gttggcgagc atcgaaccgg tcgatccggc
     5161 gctgctcgat gcccgcacgc ggtccgaggc ggaagcgcgc gttcgctcag ccgaagccgc
     5221 ccgcgagcaa gcaacagctg gcttggaaga ggctcgtgag gcgcacgaga tcgccagaca
     5281 cgagttcgag cgagtcgcgg cgcttgtcaa aaccaaagcc gtgaccgagt ccgagtacga
     5341 ccgggccgag cacgaagagc ggctgagggc ccaatcgctc cgttccgctg agtttgccct
     5401 gcgcgtggca gaattcgaac tggagattgc caaggcggcc ttcattcgca cccaaccgga
     5461 ccaagatcaa gacgccagcc aacggatgga acttcgttcg ccgatcactg gccgggtact
     5521 gcgagtgctc caggagagtg caaccgtagt cacgccgggc ctgccacttc tggaacttgg
     5581 cgacgcacaa gacatggaag tcgaggtcga tgtcctgtcg tccgatgcgg cgcggatccg
     5641 gcccggagcg cgcgtgttcc tggagcattg gggaggcgat aaaccactcg caggccgcgt
     5701 acgcctggtc gagccatcgg gcttcaccaa aatctcgtca ctgggcgttg aggagcagcg
     5761 ggtgaatgtc atcgccgact tcgtcagccc gctgtccgag cggcagggcc tgggcgatgc
     5821 ctatcgtgtc gaggcccgca tcgtcgtctg ggaaacgctg gaggccacgc tcgttcccgc
     5881 cggttgcctg ttccgccgcg gcaatgagtg ggccgtatat gtcgtggaaa acggccgtgc
     5941 ccggctcaac accgttcaag tcggccattc caacggcgtc cagaccgagg tgctcagcgg
     6001 gttagcgcct gaggcacagg tcgtgctaca tccgggcgac aaagtggtcg aaggaacatc
     6061 gattcaacgg cgaagctcca gctagtacgc acgttgatta cttaaattag ggcgatggga
     6121 cgaatggcac ttagatgtta ataactcctt tggccatatc gcgtttgact tcggcgcgtg
     6181 gttgtgtcag ccagccgtcg tggttgcgtc gctgcttctt caggcggggc tcgaagcgat
     6241 tggggcggtt agccacgcga tggctgacgc cgagcaggtg ctggaactgc cgcaagcgct
     6301 gcgtggcggc attagtcgct cgagttcgtt gttcctacac acgcgcgaaa caaggtcgcc
     6361 agatcttcct tggtcgtttg ttgggggtcc aggaaagttg tcacgacgac catgctctgg
     6421 gtgcgaaacc cacctgtgtg gcgcgaatcc tcgcttctcg tacaaggata tctgggtaac
     6481 gttggtcatt tcgaccagga tactgccgag aaacaatggt tatcaatcgt tgcgctcggg
     6541 agctccgacc atacggcaga taattaacga cgccagttag cgcgcggagg taagcagacc
     6601 gcctgcagtt tcgccatcgc aaactcgcag cggttgcatc gcccgctggg gattcagccg
     6661 gtcgccagca gcgcttttcg ccccaaatca ggttaacctg ggcgcatctg tgcaacctgc
     6721 gcgggttgct tcatcgtcac acgagcggcg ggaggccatg cgggaggccg ttccgaaagc
     6781 tgtcggctga cggagccggg tacgggatgc ggacgccgac ggccggggag tcgtcgtcat
     6841 gtccatcagc ctggaaaaag tcgcggagaa gtacctggtc gccaagaagc tctcgaccgg
     6901 gacgcagaag gagtaccggt cgaccgttac gaaatggctt gcttggggcc aaggagtcga
     6961 tgtcgatcaa attgacaggc ggcacattcg ggactatctc gattgggtcc acgacaaggc
     7021 aaccaacgat ggcggctcga acgccggtcg gaccgccaac aaggcccgcg agaatctgcg
     7081 ggcgatcctg tcctgggctt gggagcagga ctacgtggag aaattgccac gatttcccaa
     7141 acccaagccg cagcgcgatg tggccggccg tcactacctg acgaagtcgg acctgaatgc
//
LOCUS       DASUGB010000743         7371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1887591, whole genome shotgun sequence.
ACCESSION   DASUGB010000743 DASUGB010000000
VERSION     DASUGB010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887591"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFV87_14250"
     CDS             complement(<1..193)
                     /locus_tag="VFV87_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104976.1"
                     /translation="MRLPALFATLAVALLPLVALAEEKPAETAFTIGEGKLALKAPAG
                     WKKVQPRSRIIEAEFEIPTV"
     gene            complement(334..783)
                     /locus_tag="VFV87_14255"
     CDS             complement(334..783)
                     /locus_tag="VFV87_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5104977.1"
                     /translation="MYQVKDAMCGKVVSILPEASVEDAIHTLLNNKISGAPVIDAAGR
                     LCGIISQFQLLEIMYDPQIRTQSVKQFMTRDVVTVEESAFLGTAANLLVLHRIHRLPV
                     VNGGRVVGIISRSDLLKYFVATGEEIEAFFDTLKSAGANAAEPCGVR"
     gene            complement(934..1704)
                     /locus_tag="VFV87_14260"
     CDS             complement(934..1704)
                     /locus_tag="VFV87_14260"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEX5104978.1"
                     /translation="MGIRFRCPNGHKLNVKSFLAGKKGVCPKCGTSMRIPAASAEAEG
                     EADDVAETEVATVGTAAGAKGANGSAHGVPAGVPVGVAAAAVSTAPAGSAIPFGKPAP
                     PAAPVARAAVAMPAMPAHHPGPLPPAVAASDPIAEAPAATWYVRPPSGGQYGPARGDV
                     MRKWVAEGRVSGDSLVWREGWADWRNASQLFPNLAAANPAAAAAPAVPTTTATRSANR
                     YHAKKKSGSGLAVAALIVLALVCVGLVGVLVYVLTTST"
     gene            1863..2438
                     /locus_tag="VFV87_14265"
     CDS             1863..2438
                     /locus_tag="VFV87_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5104979.1"
                     /translation="MPEQTEAEAPLEIAICGDLSQHEHDIHEKLLSVPPGGECVLYFN
                     SPGGSAYAALSLASLIVLRGMQATGVVVGECSSAAIWPLAACRRRIVTAHSVLLFHQL
                     KWESGEHVDISEAAEWARHFAYLEQDMDGVLARLLGISPDKLAAWTRPGRYVTGRELA
                     AAGLAELVELQPVEALAPPRQPSTGKRKRKR"
     gene            2472..3905
                     /locus_tag="VFV87_14270"
     CDS             2472..3905
                     /locus_tag="VFV87_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX5104980.1"
                     /translation="MAGPALEYAYRYRRPSELLTSRAGPLLALATFRGLGEHPYFFEG
                     RVARPRLTADLLRALMDVVRARFHIPAATLAKIVAAADPVVTCNSDRLRFEAFSGCAS
                     TYARVDFLPEAVETETFGRGTTNVDFHAPMIAALAKVRDRDRLSLAVGAKSVELKTND
                     EAVVEKKVALPVRWLKGFVEVQAYQPRLVPVHELAGVEALRFLRGLPRMKTGKHAIHV
                     VPAGRGLRLSQQPAREGVKVGGLERLRVLERLAPHAKSLRVYGDEDSGTSGWELDLGV
                     ARFHLVLSPDVWRGFSGEGQVLSKLADKKWVAALPKVQKALAWQATIDEEGLSRQTKL
                     TPPQIRLALAALAARGLVGYDLATGAYFHRELPFDLSLVDQLQPRLVAAKKLLAAGGV
                     KPLTPTRSTSEGQPTPTRSVSEGVPSSDTELEFLVAGSGVEHRVRLSAAGSRCTCPWY
                     SKYQGQRGPCKHVLAVQMFADNESPTE"
     gene            3920..6967
                     /locus_tag="VFV87_14275"
     CDS             3920..6967
                     /locus_tag="VFV87_14275"
                     /inference="COORDINATES: protein motif:HMM:NF040119.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HEX5104981.1"
                     /translation="MPDLSRSDRLQAILDKADEAACAKFFDGATEAERRAVAKTAIDC
                     YKRFKKPEPGMPSYHPNPRLPAAGIAVLATASLSELKPFGWRLLPDAKTAFAVLTARR
                     PEWLSEYIDWLLGETAFCWPLARALIRRKLCRRPTHENYTLGMIISIDGWRLGHLHVD
                     EPQKSVGASLRADPDLLQDEIWELFRVEGQGELTLAAHDKYIAQKDTDYGWAGALAAL
                     SKTGEVSRDRLLDESLEALNRGFSQFRVAWFSQFHELLEPTVKERLKRQDKYLVLLAS
                     AIPPTVTFALDALDAIDKEKPLPAEALIEALQPVLLARQKGTARRAVQWLDRLATREP
                     ACASPAAGAAAQALAHESADVQKAAIEVLEKHGSPSDAGLKKQVASAASGVAASLRKR
                     LDKWLGGGAAEKPAPPAKSAAKAKAGGGLADLERQAAALPAKWSKLAGIPPLLAAAKK
                     GQLEIAALTFGPLDVPRLDPAAALQPVQTIDEWIDVCAHFLETPDEIDEGERVLEGLS
                     RLCGQSLNDLARELGPLKKRVDKLYRSHCGPFLGVSLRDDVCGIVRAWTTGETITGVR
                     KKTEHGSLAWFYSDKERPVWVGSEPPAHLVITQRSQQLAERAAQRLARPLLSAATHRG
                     GWLDPLVFADRLLAWEKLGEEPPHCDAIVALLRLAPDNRAAALKRLGKLKGELADAAR
                     YALGAEKPAIGKTVALWIAAARARDPQADDPLVEKKFPDLGPGGGTAARYEWSFRPGS
                     HTHAGKTYHFVRYRLEIEPKMPKQVESDLPTVLMNRLPEYVSRGEAAEIRWRASLWPA
                     GREAHLVRGVRLLANNVDWSEAEWGNKVFLEPLLAPDEPLGELARVALCLGLSAKEPG
                     EHTLAVDALIAAIADGRVDGQNLAPTLAKLLTTGDIKPGRWAKVFGDVARVSPLHGHI
                     VQLMLENTLAAAGQALADPPKDLHHVLELLIELHAESGTAIESKSVRDALTNITASGK
                     AGKAAKALLAVTGENSGGDDSEPIRAALAGRIERAKRWAAGSS"
     gene            7066..>7371
                     /locus_tag="VFV87_14280"
     CDS             7066..>7371
                     /locus_tag="VFV87_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104982.1"
                     /translation="MDRSGIRLAFLLILPLLGCAESSAPFTAVENPVQQQAARYAELP
                     GLASSPHAGLKAELALLTEERMTPAALDAQLARASNAMRASPPYHALGEAIPAISRPM
                     "
BASE COUNT         1280 a         2458 c         2459 g         1174 t
ORIGIN      
        1 taaccgtcgg gatctcgaat tccgcctcga taatgcggct cctgggctgg actttcttcc
       61 agccggcggg ggctttcaag gcgagcttgc cttcgccgat cgtgaaggcg gtttccgcgg
      121 gtttttcttc ggccagagcg acaagcggca gaagcgcgac ggcaagagtg gcaaacaggg
      181 caggcaggcg catagcggga ggtcctttgt tcgttgggaa aaggcgcgag agggcagcct
      241 cgctgcccag tcgccatttt acgtccccgc gggggcaaac caacacccta gcgcgcaccc
      301 aggaatcggc tccgtggaag aaaggaaggc ggtttaccgc acgccgcacg gttcggcggc
      361 attggcgccg gcgcttttga gcgtgtcgaa gaaggcttcg atctcttcgc ccgtggcgac
      421 gaagtatttc aggaggtcgc tgcgggagat gatgccgacg acacggccgc cattgaccac
      481 cggcagccgg tggatacggt gcaggacaag caggttcgcg gcggtcccga ggaaagccga
      541 ttcttcgacg gtcaccacgt cgcgggtcat gaattgcttg acggactgcg tgcggatctg
      601 gggatcgtac atgatctcca gaagctggaa ctggctgata atgccgcaca ggcggccggc
      661 cgcgtcgatg acgggagcgc cgctgatctt gttgttcagc agcgtgtgaa tggcgtcctc
      721 gacgctggcc tccggcagaa tggagaccac cttgccgcac attgcatctt tgacctgata
      781 cacgatcgaa ctccgcacga gtgattagct gattggagcc gacccccaat cctgcggaag
      841 tgtggcatac ggatgcggca ggggcaaaac gcttctggtg cagagcctgc agttgcgctt
      901 tgaacggcgc atccggcaca acccgcgcgg gtgttacgtg ctcgtagtca ggacgtaaac
      961 cagcacgccc accaggccca cgcagacgag ggccagcacg atgagcgcgg cgaccgccag
     1021 cccgctgccg ctcttcttct tggcgtggta gcggttggcg gagcgtgtgg cggtggtcgt
     1081 gggaacggcg ggagcggcgg cagcagccgg attagcggcg gcgagatttg ggaacaattg
     1141 gctggcattg cgccaatcgg cccagccttc acgccagacg agcgaatcgc cgctgacacg
     1201 gccctcggca acccacttgc gcatcacgtc accgcgagcc gggccatatt gcccaccaga
     1261 tggcgggcgg acgtaccagg tcgctgccgg agcctcggca atcggatctg atgcagctac
     1321 cgccggaggc agcggccccg gatgatgagc gggcatggcg ggcatcgcta ctgcagcccg
     1381 cgcgacaggc gccgcgggcg gggccggctt gccgaaggga atcgcgctgc cggctggcgc
     1441 tgtagaaact gcggctgcgg caactcccac gggcacgcca gccggcaccc cgtgcgccga
     1501 gccgttggcc cccttcgctc cagccgccgt gccgaccgtg gccacttcgg tctcggcaac
     1561 gtcgtccgct tccccctcgg cttccgcgga ggcggcagga attcgcatgc tggtcccgca
     1621 tttggggcaa acgccctttt tgccggccag aaacgacttg acgttgagct tgtgcccgtt
     1681 ggggcagcga aatcgaatgc ccatcttctg ccgtaattca ggcccccgcg gcccagcttt
     1741 cgggcggcga cctcgcgcgc cgcgcccggc gaaagcaatc cccattatac tcgtcccgcc
     1801 agggcacgca ttattccagt cggctgcaaa tccggcacaa tcggatctcc gtggaactcg
     1861 ccatgccaga acaaaccgaa gccgaggccc ctttggaaat cgccatttgc ggcgatctct
     1921 cgcagcacga gcacgacatt cacgagaagc tcctctcggt gccgccgggg ggcgagtgcg
     1981 tgttgtattt caactcgccc ggcgggagtg cgtacgcggc gctgtcgctg gcgtcgctga
     2041 tcgtgcttcg cggcatgcag gcgacgggcg tggtcgtcgg cgagtgctct tccgccgcga
     2101 tctggccgct ggcggcttgc cggcggagga tcgtgacggc tcatagcgtg ctgctcttcc
     2161 accagctcaa gtgggaaagc ggcgagcacg tcgatatctc cgaagccgcc gaatgggctc
     2221 gccacttcgc ctacctggag caggacatgg acggcgtcct cgctcggctc ttggggattt
     2281 cgcccgacaa gctggccgcc tggaccaggc ccgggcgcta cgtcaccggt cgcgagctcg
     2341 ccgcagccgg cctggccgag ctcgtcgagc tgcagccagt tgaagcgctt gccccgccgc
     2401 gacaacctag cacggggaag cggaaacgga agcggtagag ctgagatcgc cttgctgcga
     2461 ggggacaact gatggctgga ccggcgctgg aatacgctta ccgctaccgc cggccgtccg
     2521 agctgctgac gtcacgagcc ggaccgttgc tggcgctggc gacttttcgc ggcctgggcg
     2581 agcaccccta ctttttcgag ggccgcgtcg ccaggcctcg gctgacggcg gacctcttac
     2641 gggcactgat ggacgtggtg cgggcccgat ttcacattcc ggccgcgacg ctcgccaaga
     2701 tcgtcgccgc cgccgatccg gtcgtcacct gcaacagcga ccggctgcgg ttcgaggcct
     2761 tctccggctg tgcgagcacc tatgcccgcg tcgattttct gccggaggcg gtcgagacgg
     2821 agaccttcgg ccgcggcacc acgaacgtcg atttccacgc accgatgatt gccgcgctgg
     2881 ccaaggtgcg cgaccgcgac cgcctgtcgc tggccgtcgg ggccaagagc gtcgagctga
     2941 aaaccaacga cgaggcggtc gtcgagaaga aggtcgccct gcccgtccgc tggctgaagg
     3001 ggttcgtcga ggtgcaagcc tatcagccgc ggcttgtgcc ggttcacgag ttggcaggcg
     3061 tggaagcgct gcggttcctg cggggcctgc cgcggatgaa gaccggcaag cacgcgattc
     3121 acgtcgtccc cgcgggccgc ggcctcaggc tcagccagca gccggccagg gagggcgtga
     3181 aggtcggcgg cctggagcgg ctgcgggtgc tggaacggct cgctcctcac gcgaaatcgc
     3241 tgcgcgtcta tggcgacgaa gactctggca caagcggctg ggaattggac ctgggcgtgg
     3301 cccgctttca tctggtgctc agccccgatg tgtggcgcgg cttctcgggc gaagggcaag
     3361 tcctgtcgaa gctggccgat aagaagtggg tagcggccct gcccaaggtg cagaaggccc
     3421 tcgcctggca ggcgaccatc gacgaggagg gactctcgcg gcagacgaag ctcacgccgc
     3481 cgcagattcg cctggccctg gcggcactcg cggcccgtgg cctggtcggc tacgacctgg
     3541 cgaccggcgc gtacttccat cgcgagctgc cgttcgatct ctccctcgtc gatcagcttc
     3601 agccgcggct cgtcgccgcc aaaaaactcc tcgccgccgg cggcgtgaag ccgctcacac
     3661 caacccgaag cacaagcgag ggccagccca caccaacccg aagcgtcagc gagggagtgc
     3721 cgtcgtccga taccgaactc gaattcctcg tcgccggcag cggcgtcgag catcgcgtcc
     3781 gcctctccgc cgccggcagc cgctgcacct gcccctggta ttcgaagtac caaggccagc
     3841 gcggcccctg caagcacgtg ctcgccgtcc agatgttcgc cgacaacgaa tcgcccaccg
     3901 aataaccccg gagattcgca tgcccgacct ctcccgctcc gaccggctcc aagccatcct
     3961 cgacaaggcc gacgaagccg cctgcgcgaa gttcttcgac ggcgcgaccg aagccgagcg
     4021 gcgcgccgtc gccaagacgg caatcgattg ctacaagcgg ttcaaaaagc ccgagccggg
     4081 catgccgagc taccatccca atccccgcct gccggcggca ggaattgccg tcctggcaac
     4141 cgcgtcgctg agcgagttga agccgttcgg ctggcggctg cttcctgatg cgaagaccgc
     4201 atttgccgtg ctcaccgcgc ggcggccgga atggctttcg gaatacatcg attggttgct
     4261 tggcgagacc gcgttctgtt ggccgctggc ccgcgccctc attcgccgca agctctgccg
     4321 gcgtccgacg cacgagaact acacgcttgg catgattata tcgatcgacg gctggcggct
     4381 gggacatttg cacgtcgatg agccacagaa gtctgtcggc gcaagtttgc gcgccgaccc
     4441 cgacctgctt caagacgaga tctgggagct gttccgcgtg gaaggacagg gcgaattaac
     4501 gctggccgct cacgacaaat acatcgcgca gaaggacacc gattacggtt gggcgggagc
     4561 cctggccgcc ctgtcgaaga ccggcgaagt ctcccgcgac cgcctcctcg acgaaagcct
     4621 cgaagcgctc aaccgcggtt tctcgcagtt ccgcgtggcc tggttctcgc agtttcacga
     4681 gctgttggag ccgaccgtca aggagcggtt gaaacgacaa gacaagtatc tcgttctgct
     4741 cgccagcgcg atcccgccga cggtcacgtt cgccttggat gcgctcgacg cgatcgacaa
     4801 ggaaaaaccg ctcccggctg aagcgctgat cgaagccctc cagccggttc tgctcgcccg
     4861 gcaaaaggga acggccaggc gggccgtgca atggttggac cggctggcga cgcgcgagcc
     4921 ggcctgcgca tcgccagcag ccggcgcggc cgcccaggcg ctcgcccacg aatcagccga
     4981 cgtgcagaag gcggcgatcg aggttttgga gaagcatggc tcgccgagcg atgccgggct
     5041 gaaaaaacag gttgccagcg cggcctccgg cgtggccgca tcgctccgca agcggctcga
     5101 caaatggctg ggcggcggcg ccgcggaaaa gcccgctccg cccgcgaaat cggcggcaaa
     5161 agccaaggcc ggcggcggcc tcgcggacct ggagaggcaa gccgccgcgc tccccgccaa
     5221 gtggtcaaag ctggccggaa ttccaccgct gctggcggca gccaagaaag ggcagctcga
     5281 gatcgcggct ctgaccttcg gcccgctgga tgtcccgcgg ctcgatccgg ctgccgcact
     5341 gcagcccgtg cagacgatcg acgagtggat cgacgtctgc gcccactttc tggaaacgcc
     5401 cgacgagatc gacgaaggcg agcgggtact cgaagggctt agccgattgt gtggccaatc
     5461 gctgaacgac ctggcccgcg agctgggccc gctgaagaag cgggtcgaca agctctaccg
     5521 cagtcattgc gggccgtttc tcggcgtgag cctgcgcgac gacgtgtgcg gcatcgtgcg
     5581 ggcctggacc actggcgaaa cgataaccgg cgtccgcaag aagaccgagc acggcagtct
     5641 ggcgtggttc tattccgaca aggagcggcc tgtgtgggtc ggatcggaac cgcctgcgca
     5701 cctggtcatc acgcagcgct cgcagcagct cgccgagcgg gccgcccagc gcctggcgcg
     5761 ccccctgctg agcgccgcca cacaccgcgg cggatggctc gatccgctcg tcttcgccga
     5821 ccggctgttg gcgtgggaga agctcggcga agagccgccg cactgcgatg caatcgtcgc
     5881 gctcctccgg ctcgcgcccg acaaccgcgc ggcagccctc aagcggctgg gcaagctgaa
     5941 gggcgagctg gccgacgcgg cccgctatgc ccttggagcc gagaagccgg caatcggcaa
     6001 gactgtggcc ttgtggattg ccgcggcccg cgcccgcgat ccgcaggccg acgatccgct
     6061 cgtcgagaag aagttccccg acctcggccc gggtggcggc acggctgctc gctacgagtg
     6121 gagcttccgg cccgggtcgc acacgcacgc gggcaagacc taccacttcg tccgctatcg
     6181 tctcgaaatc gagccgaaga tgcccaagca ggtcgaaagc gatctgccga cggtcttgat
     6241 gaaccgcctg cccgagtatg tgtcgcgagg cgaagctgcc gagattcgct ggcgcgcctc
     6301 cctctggccc gctggccgcg aggcgcacct ggtccgcggc gtgcggctgt tggcgaacaa
     6361 tgtggactgg tccgaagccg agtgggggaa caaggtgttc ctcgagccgc tgctcgctcc
     6421 cgacgagccg ctgggcgaat tggcccgcgt cgctctttgc ctgggccttt ccgctaagga
     6481 gccgggcgag cacacgctgg cggttgacgc gctgatcgca gcgatcgccg atggccgcgt
     6541 cgatggccag aacctcgctc caacattggc aaagctattg acgaccggcg acatcaagcc
     6601 cggccgctgg gcgaaggttt tcggcgacgt cgcccgcgtc tcgccgctgc acgggcacat
     6661 cgtgcagctg atgctggaaa acacgctggc cgcagccggc caggcgctgg cagacccgcc
     6721 caaggacctg catcacgtgc tggaattgct catcgagctg cacgccgagt cgggcacggc
     6781 catcgaaagt aagtccgtgc gcgacgcgct aaccaatatc acagccagcg ggaaagcagg
     6841 caaagcggcc aaggcactac tggcagtgac aggcgagaat tccggcgggg acgacagcga
     6901 gccgattcgc gccgcgctgg ccggccgcat cgaacgggcc aaacgctggg ccgcggggag
     6961 cagctagaac gtacgcctcc gccgtcttga gcagccgatt gctattccgc cgccatccgg
     7021 cgacgaccta caattcggcg ccattctcgg caggagccaa tcgcgatgga tcgcagcggg
     7081 atcagactgg catttctcct tatactgccg ctgcttggat gcgcggaatc cagtgctcca
     7141 ttcacggcgg tcgagaatcc cgtccagcag caggcggcca gatatgcaga gctgccgggg
     7201 ctggcgagct cgccgcatgc ggggctgaag gcggaactgg ccctgctgac tgaggaacgg
     7261 atgaccccgg cggctctcga cgcgcagctt gctcgggcgt cgaacgccat gcgcgccagc
     7321 ccgccgtatc acgccctcgg cgaagcgatc ccggccatat cgcggccgat g
//
LOCUS       DASUGB010000744         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_222473, whole genome shotgun sequence.
ACCESSION   DASUGB010000744 DASUGB010000000
VERSION     DASUGB010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222473"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFV87_14285"
     CDS             complement(<1..765)
                     /locus_tag="VFV87_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104983.1"
                     /translation="MSWRRNLIGGGTIIALVLAVIVVWKNYRSPQDRFVEELELVGFS
                     TSMIGGPTAERLDAALSESDRLRVDLFEQYSKRVDSDRRYQLAVLLIERESLEYAEHA
                     AARIHEVPWPEVRIWRIYAKSDSLSAEYRRKIQQLLLASPTSEAKLWAAKWHIQQGNE
                     ELAEDCYYSAMTNGLFWDALDAADKLVKSPRYRHDALHHLLAAVAESESFVSRAAFTL
                     MTELGLKDELKQLLADCKMKNADPTVRRALVERLADA"
     gene            complement(777..2210)
                     /locus_tag="VFV87_14290"
     CDS             complement(777..2210)
                     /locus_tag="VFV87_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5104984.1"
                     /translation="MYPCQRYTPAPASRRTFLRQAGCGFGAVALAALWAQNGIGGAEI
                     ASTDPLAPKSPHFKPRAKNVIFLYMDGGPSQVDTFDPKPRLNKEHGQPFAVKIEPTQF
                     NNNGSTFGCPWKFQQYGQSGIPVSELFPNVAKHVDKLAVIRSMTSQFSEHTSANYFLH
                     TGTGVQGRPSMGAWFGYGLGSECRDLPGFIVINGGLIPPGGLDNFNSGFLPATYQGSI
                     FTASDPPVANIVRTEATDQLQRNKLALLKRLDAQVQERLGKVDAVESAIANYELAYRM
                     QTVVPGLMDMQDETEATQKLYGLDATTKGTASFARQCLLARRLVERGVRFVELTCPSI
                     GHDRWDQHGKLKEGHEDNARAVDQPIAALLEDLHQRGLLDETLVVWSGEFGRTPFAQG
                     SDGRDHNPFGFSLWLAGGGVKGGTIHGQTDEFGYKAIENVAEIHDLHATMLHLLGLDH
                     TKVTFRFSGRDMRLTDVHGHVIHDVIA"
BASE COUNT          504 a          789 c          833 g          565 t
ORIGIN      
        1 ggcgtccgcc agccgttcaa caagggctcg tcgcacggtt gggtccgcat tcttcatttt
       61 gcagtctgct aacaattgtt tcagttcatc tttcaagcca agttctgtca tcagtgtaaa
      121 ggctgctctt gagacgaagc tctcgctctc ggcgacggcg gcaagcaagt gatgtagggc
      181 gtcatggcgg tagcgcggtg atttgaccag cttatcggcg gcgtctagcg cgtcccagaa
      241 aagtccattg gtcattgccg aatagtagca gtcttccgcc aactcctcat tgccctgctg
      301 tatgtgccat ttggcagccc agagttttgc ctcagaagta ggtgacgcca acaacaattg
      361 ctgaatcttg cgccggtact cggctgacag ggaatccgat ttggcataga tgcgccagat
      421 tcggacctcc ggccagggaa cttcgtgaat gcgagccgct gcgtgttctg cgtattccag
      481 cgattcacgc tcgatcagca gtacggccaa ttggtatctg cgatccgagt caactctctt
      541 tgaatactgc tcaaacaaat cgactcgcag gcgatcggat tcactgagag cggcgtcgag
      601 tctttcggca gttgggccac cgatcatcga cgtactaaag ccaaccaatt ccaactcttc
      661 cacaaagcga tcttgaggtg atctatagtt cttccacaca acgatcaccg cgagcaccaa
      721 ggcgatgatc gttccaccgc cgatcaagtt gcgtcgccaa ctcatagttc gaggccctat
      781 gcaatcacgt catgaatcac atgcccgtgt acgtcggtca gacgcatatc gcggccggag
      841 aagcggaagg tgaccttcgt gtggtcgagg ccgaggaggt gcagcatcgt ggcgtgcagg
      901 tcgtggattt cggcaacgtt ttcgatcgcc ttgtagccga attcatcggt ctggccgtgg
      961 atcgtgcccc ctttgacgcc gccgccggcc agccagaggc tgaagccgaa ggggttatgg
     1021 tcgcggccgt cggagccctg cgcaaatggc gtgcggccga attcgcccga ccagacgacg
     1081 agcgtttcgt ccaggaggcc ccgctggtgg aggtcttcga gcagggcggc aatcggctga
     1141 tcgacggcgc gggcgttgtc ttcgtgcccc tccttgagct tgccgtgctg gtcccagcgg
     1201 tcgtggccga tgctggggca cgtcagctcg acgaaccgca cgccccgctc aaccagccgg
     1261 cgggccagga ggcactgccg cgcgaaggac gcggtgcctt tggtcgtggc gtccaagccg
     1321 tagagcttct gcgtggcttc ggtctcgtcc tgcatgtcca tcaggccggg gacgaccgtt
     1381 tgcatccggt aggccagctc gtagttggcg atcgccgact cgaccgcatc gaccttgccc
     1441 aaccgctctt gcacctgggc atcgagccgc ttgagcaggg cgagcttgtt ccgctggagc
     1501 tggtcggtcg cttcggtacg gacgatgttg gcgacgggcg gatcggacgc cgtgaagatc
     1561 gagccttgat acgtcgccgg caggaagccg ctgttgaagt tgtcgaggcc gccgggcggg
     1621 atcagcccgc cgttgatgac gatgaagccc ggcagatcgc ggcactcgct ccccaggcca
     1681 tagccgaacc aggcccccat gctcggccgg ccctggacgc ccgtgcccgt gtgcaggaag
     1741 taattggccg aggtgtgctc ggagaattga ctggtcatcg agcggatgac ggccagctta
     1801 tcgacatgct tcgccacgtt cgggaacaac tcgctgaccg gaatgccgct ttggccgtac
     1861 tgctggaatt tccacgggca gccgaacgtg ctgccgttgt tattgaactg cgtcggctcg
     1921 atcttcacgg cgaacggctg gccgtgctcc ttgttcagcc gcggcttggg gtcgaaggta
     1981 tcgacctggc tcggtcctcc gtccatgtag agaaagatga cgttcttggc ccgcggcttg
     2041 aaatgggggg actttggagc gagggggtcc gtgctggcga tctccgctcc gccgataccg
     2101 ttttgcgccc atagggccgc cagcgcgacc gctccaaaac cgcaaccggc ctggcggagg
     2161 aaggttcttc gtgacgcggg tgctggagtg taccgctggc agggatacat cgtaagaaat
     2221 caaatcaaaa gtctggaagt cgtgacggcg gatcttcttc gaggagggcc gctcgaagcc
     2281 tgattcgcca aggatcgttc atctgcaaga gttcggcggt tgctcgtcct tctggagtga
     2341 tgcccgcaat gatctcaccc tccatccgaa agtgcccctc ccaacggtcg tgcctggggt
     2401 caaacagtcg aaccaacgcg tccgtcagga tcgtaggaac tggcgtttgg acccttaaaa
     2461 tgattgcagc gactgcaagc ccaacacaga ctgtccaaat cgtcggaacc gcgatgctgc
     2521 cgggctcgga tatgctcgat gtgaaagggg agcaatccgc tggcagcctc cggcaatcgg
     2581 cagtattcgc agcgcatttg cgcgagagat ctgacgagtt gccgtgtcgc ttcgtccatc
     2641 aggaggattg ttcgcgaagt acctggcggg ccttggcctg caaaatcgcg a
//
LOCUS       DASUGB010000745         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2028080, whole genome shotgun sequence.
ACCESSION   DASUGB010000745 DASUGB010000000
VERSION     DASUGB010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028080"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFV87_14295"
     CDS             <1..301
                     /locus_tag="VFV87_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104985.1"
                     /translation="KKGNPRRHDGNCNRQPDHPSRQAAERFLTEWLARGYDQHAQPEA
                     DKPAGCELQQAIMNIPCKYSGGPGAWAYDQGHRHPDQPRLTDAEYEAIRPFIVTT"
     gene            330..821
                     /locus_tag="VFV87_14300"
     CDS             330..821
                     /locus_tag="VFV87_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104986.1"
                     /translation="MKIYVASSWKNQHAVELITAGLRERGHEVLSFVENNHGEVQGHK
                     ATEDGKPMALEEWVWSERGLKSFNYDTRGATESDLVIYVGPSGCDAWAEVGAAWGRGV
                     PVLGLVSKGEQVGLMRRMVSHWHENVRSLWDEIDSLKRRDTHSRSDVPCPACGDLDGC
                     HKT"
     gene            902..1078
                     /locus_tag="VFV87_14305"
     CDS             902..1078
                     /locus_tag="VFV87_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104987.1"
                     /translation="MPDTWIRTSQRLPEKNQKVEWISPGGIQERGTFAGGLIWFPEGS
                     SMYVYYTPEFWRPA"
     gene            1330..1632
                     /locus_tag="VFV87_14310"
     CDS             1330..1632
                     /locus_tag="VFV87_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104988.1"
                     /translation="MAPQDHPICHGICAAMEAEPKKGVGSLVLKLIDLFKVNMDSLRT
                     IFPGEDAIVAFAKEQFDKYIRPIRVLPSFVGEAAEKAFDDGVWWAVEQAIRALVPG"
     gene            1674..1982
                     /locus_tag="VFV87_14315"
     CDS             1674..1982
                     /locus_tag="VFV87_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104989.1"
                     /translation="MIEYLTTHWNVVLGIVIAVAVLFGGKLQPLAAQAWEWLKRQATP
                     AAPVPLPVPPPEEADPDVLDMLAIKRLRNRRCYDQCPEAKKALDVLMTHFFHETPPPA
                     "
     gene            1979..2362
                     /locus_tag="VFV87_14320"
     CDS             1979..2362
                     /locus_tag="VFV87_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104990.1"
                     /translation="MNDMPMWKVAALLLAAWLIFGGPKGCNGIPGLTTKATAATYVYE
                     KDSGGVPPAVGSALGKLNERGIIATTFEDDTTNASQQVPTQYKNVLPAARDAGLPALV
                     VSAGERVVRTLSKPTTEEQVLEAVP"
     gene            2359..2538
                     /locus_tag="VFV87_14325"
     CDS             2359..2538
                     /locus_tag="VFV87_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104991.1"
                     /translation="MTRLELDTYRLRLSEQLDAQLATIEARRDPADSSDFALILSNQT
                     LLLRAMVLLLAQPEA"
     gene            2539..>3295
                     /locus_tag="VFV87_14330"
     CDS             2539..>3295
                     /locus_tag="VFV87_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104992.1"
                     /translation="MDSFTPINPKLIDARSPQVGPHDGYPDEIKAGDTPDVLLEACGA
                     AGREFPQALYIDPSNWADKARDNDKYHTWPMNYVDRFTNQSPTHECTCHALRTVAETA
                     RNKQRGIIFPDGPKADFRYEQSAKGSVWLSPLSIYSEANPRQWGGAGCQQVLNIACNR
                     GFLPEKIQPANYGFKHDLVGTTGKGGKNQSHGSWVRLGDFPSGWQETAKYFKPQEVII
                     CDRFEQVVSLVLHGYAVEVGRSGHAIPYAFWNVQ"
BASE COUNT          678 a         1019 c         1040 g          558 t
ORIGIN      
        1 caagaaagga aatccaagga gacatgacgg caactgcaac cggcaacccg atcaccccag
       61 ccgccaagcc gccgagcggt ttctaaccga gtggcttgcg cgaggatacg accagcacgc
      121 ccagcccgaa gccgacaagc ccgctgggtg cgagttgcag caggcgatta tgaacatccc
      181 ttgcaagtat tcgggtggcc ccggcgcttg ggcatacgat cagggccacc ggcaccccga
      241 ccagccgcga ctgactgacg cggagtatga agcgatccga ccgtttattg taaccacata
      301 gccccacggg gcagaaagca ggtgagggga tgaagattta tgtagcgtca tcatggaaga
      361 atcagcacgc agtggagtta atcactgcgg ggctgcgcga gcgagggcat gaagttctgt
      421 cgttcgtcga aaacaatcac ggcgaagtcc agggccacaa ggctaccgag gacggcaagc
      481 caatggcctt ggaagaatgg gtgtggtcgg aacgtgggct caagtcgttc aattacgaca
      541 cccgcggcgc aaccgaatcc gatcttgtga tttacgtcgg cccgtccggc tgtgatgcgt
      601 gggcggaagt tggcgcggcg tggggtcgcg gcgtcccggt tctcggcttg gtgtccaagg
      661 gagaacaagt cgggctgatg cgtcgaatgg tgtctcactg gcacgagaac gtccgctcgt
      721 tgtgggatga gatcgactca ctcaaaagac gagatacgca tagccgctcc gatgtcccat
      781 gcccagcttg cggcgatttg gacggctgcc acaaaaccta aaaccgcccc aacgggcagg
      841 agcgaacgat gagagtccaa ggtcgggaac cccaaccgcc cgaagggaag gagtgatagg
      901 gatgcctgac acttggatta gaacaagcca gcggctgcct gaaaagaatc aaaaggtcga
      961 gtggatttca ccgggcggca tccaagaacg cggcacgttc gccggcggct tgatctggtt
     1021 ccccgaaggc tcgtcgatgt acgtgtacta cacgccagag ttctggcgac cagcctaacc
     1081 gccatcgcac gggcggcaac gtggggcgat agcctaacga gcgtaacgaa ttttcgcccc
     1141 tcctggccag tcggtgaagc tggccgcttt ttgacacaca gagtcgctac ccgctacaat
     1201 tcgggagcca aagataggga cagcgggcgg cgactagcta tcgccgcttc tgaatcggcc
     1261 tcagttggga ggaccggcaa ttctgccgtc ctcccttttt ctttttgagg ccctacccgg
     1321 agaaacgtaa tggcccccca agaccacccg atttgtcatg gcatctgcgc tgcgatggag
     1381 gctgaaccca agaagggagt cggctccctc gtcctgaagc tgattgatct gttcaaggtc
     1441 aacatggact ctctgcggac gatcttccct ggcgaagatg cgatcgtggc gttcgcgaag
     1501 gagcagttcg ataagtacat ccgcccgatc cgcgtgctgc catcgttcgt tggcgaggcc
     1561 gccgagaagg cgttcgacga cggcgtgtgg tgggccgtcg agcaggcgat tcgtgcgctc
     1621 gttcccggct agtccacccg ttccatttcc tccccgaact ttaggccgct gccatgatcg
     1681 agtacctgac cacgcactgg aacgtcgtcc tgggaatcgt cattgcggtg gccgtgctgt
     1741 tcggtgggaa acttcagccg ctcgccgccc aagcctggga gtggctgaag cggcaggcga
     1801 cccccgcggc gccagttccg ctgccggtcc cgccgcccga ggaagccgat cctgacgtac
     1861 tcgacatgct ggcgatcaag cgcttgcgca accggcggtg ctacgaccag tgcccggagg
     1921 ccaagaaggc gctcgacgtg ttgatgaccc acttcttcca cgaaacgcct ccgcccgcat
     1981 gaacgacatg cccatgtgga aagtcgcggc cctcctgctg gccgcctggc tgatcttcgg
     2041 cggacccaag gggtgcaacg gcatccccgg tctgacgacg aaggccacgg ctgcgactta
     2101 cgtgtacgag aaggacagcg ggggcgttcc gccggcagta gggtcggcgc tgggcaagct
     2161 gaacgagcgc gggatcatcg cgacgacgtt tgaggacgac acgaccaacg ccagccagca
     2221 ggttccaacg cagtacaaga acgtccttcc ggccgcccgt gatgccggcc tgccggcgct
     2281 ggtggtcagc gccggagagc gggtcgtgcg gaccctctcc aagccgacga ccgaggagca
     2341 agtgctggag gccgtgccat gactcgcctg gaactcgaca cgtaccgcct gcgcctgagc
     2401 gagcagttag acgcccagct cgccacgatc gaggcgcgcc gcgacccggc cgacagttcg
     2461 gacttcgcgt tgattctgag caatcagaca ctgctgctgc gggcgatggt cctgctactc
     2521 gcgcagccgg aggcataagt ggacagcttc accccgatca acccgaaact gatcgacgcc
     2581 cgctcgccgc aagtggggcc gcacgacggc tatcccgacg agatcaaggc tggggatacg
     2641 ccggacgtgc tcctggaagc ctgcggcgct gccggccggg agtttcccca ggccctctac
     2701 atcgacccgt ccaactgggc ggacaaggcg cgggacaacg acaagtatca cacctggccg
     2761 atgaactacg tggatcggtt caccaaccag agcccgacgc acgaatgtac ctgtcacgcg
     2821 ctgcggaccg tggccgagac tgcccgcaac aagcagcggg ggatcatctt cccggacgga
     2881 cccaaggccg acttccgcta cgagcagagc gccaaggggt cggtttggct ttctccgctg
     2941 tcgatctaca gcgaggccaa cccgcggcag tggggcgggg ccggctgcca gcaagtcctc
     3001 aacatcgcct gcaaccgcgg cttccttccg gagaagatcc agccggccaa ttacgggttc
     3061 aagcacgacc tggttggcac gaccggcaag ggcggcaaaa accagtcgca cggatcctgg
     3121 gtccggctgg gcgactttcc atccggctgg caggaaacgg ccaagtattt caagccgcaa
     3181 gaggtgatta tctgcgatcg cttcgagcag gtcgtgagcc tcgtcttgca cggctacgca
     3241 gtcgaggtcg ggcgctcggg acacgcgatt ccctacgcct tctggaatgt tcagg
//
LOCUS       DASUGB010000746         8834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2028138, whole genome shotgun sequence.
ACCESSION   DASUGB010000746 DASUGB010000000
VERSION     DASUGB010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028138"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VFV87_14335"
     CDS             <1..1054
                     /locus_tag="VFV87_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5104993.1"
                     /translation="LQAAGADIIDVGCDPGEPWPGVADCVRALKAEGHRVSIDSLNPA
                     EIAPAAGAGAELVLSVNSTNRHAARDWGCEVIVIPDDFASLGGLEETLELLAGAGVRL
                     RIDPVIEPIGCGFAASLGRYIEVRRRYPDAEMLMGTGNLTELTDADSAGINVLLLGFC
                     QELGVRSVLTTQVINWARTSVRECDLARRLVYHAASRRIPPKHIEERLITLRDPKVHE
                     FGVETLERLAQQIRDNNYRIYAEAGEVHLLGAGLHLADRDPLLLFERLLHPGFGGRAD
                     SHQPPTNVDPSHAFYLGYEMAKAAIALALSKEYRQDEALDWGYLTVAEKSHRLRKSKA
                     ARKSADAAGGAAEDGA"
     gene            1051..2511
                     /locus_tag="VFV87_14340"
     CDS             1051..2511
                     /locus_tag="VFV87_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5104994.1"
                     /translation="MSAAASDLPLVVELQPPPDVESALLRLASQPYSLLLDSALRDPR
                     LGRYSFLSADPFELLTVPADGADGLAQLAERLKTWPAGSRPDLPPFQGGAAGLFSYDL
                     SRSLERIASPRHDEFGVPAMAIGFYDVVLAFDHAQHRAWLISQGLPEREPSRRLQRAA
                     ARIDQFQTWLAGAATDRPNVVVRSANDRPFAERTATNLAPSFPVPGPAGLLSNFTADA
                     YRAAVQRAIDYIYAGDIFQVNLSQRLLCPAAGDALSLYLRLRQCNPAPFAGWFDLGEF
                     QIASASPERFLSVRDRQVEARPIKGTRRRTTWAEADLFAGDDLLQSEKDRAENVMIVD
                     LLRNDLSRVCTPGSVHVSQLCGLEIYQFVQHLVSVVRGELAPGRSPVDLIRAAFPGGS
                     ITGAPKVRAMEIIAELEPTARGPYCGALGYIGFDGAMDLNILIRTITAGRGWWQFPVG
                     GGIVAQSSPQREYEETWHKAEGLLRALVPMKLGPLA"
     gene            2508..3170
                     /locus_tag="VFV87_14345"
     CDS             2508..3170
                     /locus_tag="VFV87_14345"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5104995.1"
                     /translation="MILLIDNYDSFVHNLARYLRRMGQETLVVRNTEIDVAGIEELAP
                     QAVVLSPGPCTPNEAGCSLNVVRSLGASVPILGVCLGHQTIGAALGAKVVRAPQPVHG
                     RASLVRHDGAGVFAGLPSPLTVGRYHSLIVDPATLPEDLVPTAWSDEGVVMALAHRRW
                     PLVGVQFHPESILTECGYELLANFLRLAGLHAESAQHEMNGERRIVEQASPALPLQPV
                     TF"
     gene            3298..3903
                     /locus_tag="VFV87_14350"
     CDS             3298..3903
                     /locus_tag="VFV87_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HEX5104996.1"
                     /translation="MPAEPDELILEGIVVTQDAAASANIAPMGPRVDREQTRLVLRPF
                     QTAQTFRNLQATRCGVFHVTDDVELLARAAVGQLDPPSALVPVDGFSCLRLADCCRWL
                     AFRVNAIDDQSERTTIDCQVIAKGTLREFFGFNRAKHAVVEAAILATRIGIVPDAELW
                     AEMARLQSPVAKTAGEQERRAFEFLRQYVESRLGPSFPDSD"
     gene            3919..4905
                     /locus_tag="VFV87_14355"
     CDS             3919..4905
                     /locus_tag="VFV87_14355"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5104997.1"
                     /translation="MPRLVRVAAPSRLHFGLWSLGGEGRQFGGVGAMVERPGLVLEIA
                     PALEFAATGPLAARAGVFARRWAEFHRQPLPACALRVAEAPPEHVGLGTGTQLGLAIA
                     AGMSAWCGLPAQSPLELAVSVGRAERSAIGTHGFVLGGLIVEQGKLPGEPVSPLDCRI
                     ALPEAWRFVLVRPLGQAGLAGEDEAQAIAALPPISDAVRERLISEARDGLVPAAATGD
                     FRTFAASLYQYGNLAGQCFKARQGGPYNGPVLTALVERIRSLGFTGVGQSSWGPTLFV
                     ISENEASARRLAAELAADALEILVTAPSNSGARIEVRDPDPNPANPAAVQRP"
     gene            4902..6350
                     /locus_tag="VFV87_14360"
     CDS             4902..6350
                     /locus_tag="VFV87_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5104998.1"
                     /translation="MNSRLRTTALACALSTVLLGGCPFSEQSNDPSPEPKDTQPLRLV
                     VVDDPALGAAIASEWQAQTEQSLETEQMTAAELAQAKLLPGDIVLFPTGMLGHCIEGG
                     LIVPLSDDLLAAPQFDRRDIFDQIRLREMTWGNRTVAVSLGSPQILLAYRADLFEKLG
                     LQPPADWAAYQELALKLAERAALGEAAPPDDQPWHATLEPLADGWAGQVLLARAAAYA
                     MHPDQLSPLFNSDDAQPLIDRAPYVRALKELTAAAKVGGFADTRLTPQEALAELRAGH
                     AAMALGWPAPPAGKVADKETTTGTIRYALLPGAKQSYNFATKRWTTSESGEPTRVPVL
                     ACSGRLAAVTSNSAARQRAESFVLWLGGKDASRQVAPRSSATAPYRQSQLREVELWTE
                     GLDPGEARQYGVTLAAALSIPRVFPGLRLPGRLDYVAALDKAVQQAAAEQAAPDEALQ
                     HAAARWAEITNAIGADKQQQAILRSLSQADLK"
     gene            complement(6357..6677)
                     /locus_tag="VFV87_14365"
     CDS             complement(6357..6677)
                     /locus_tag="VFV87_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family transcriptional regulator"
                     /protein_id="HEX5104999.1"
                     /translation="MPIYVYELITDDDSPGEQFEIFQKMADPPLTKHPESGQPVRRVF
                     CPPAIGGQWSDSAMAKNVADDKKLDRMGFTKYVKAGDGHYEKTAGTGPDVISRDQAIS
                     AEDI"
     gene            complement(6876..7445)
                     /locus_tag="VFV87_14370"
     CDS             complement(6876..7445)
                     /locus_tag="VFV87_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105000.1"
                     /translation="MPLDITARMRLICDDMIARLPELAHIDMPRVAVAFSQARNGERH
                     GLFASLTPLRFQDGAQVGRRRGRSYRIQSLRDKSGREMLYILRFCLPRFLDLDLREKL
                     VTVLHELWHISPAFNGDIRRHAGRYHAHTHSQAEYDEQMGLLADRWLGHQPPAELWGF
                     LRSDFRELVARHERIVGIRIPRPRLMPVE"
     gene            complement(7538..8125)
                     /locus_tag="VFV87_14375"
     CDS             complement(7538..8125)
                     /locus_tag="VFV87_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HEX5105001.1"
                     /translation="MRHRKRGRVLGRAPSHRKAMLKNLASALILTERDAEDDDNTPKV
                     KGRVVTTLAKAKEVRPLVERCITLACKSLEHVEAAKEHGTDAARNSDAWKKWRESEKW
                     QSWAKAISPAVAARRRARQLLGDKVAVKVLFAELAPRFRDRKGGYTRVVKLAKPRLGD
                     AGPRAIIEFVGVRDRVVQRSEKPAFSDEGAEPAKA"
     gene            complement(8231..>8834)
                     /locus_tag="VFV87_14380"
     CDS             complement(8231..>8834)
                     /locus_tag="VFV87_14380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5105002.1"
                     /translation="HVLATLTDNVPFMMEMVVENGRGYIPATEHSTGDHEIGIIPIDA
                     VYSPVTRVRYEVEETRVGQKTNYDKLTLEMWTNGSVHPEMALVEAAKILRKHLNPFVQ
                     YAELGPRVHAPARGATLGVDGAMEAKLSMSLAELKLSVRAMNCLESENINTVRDLVQK
                     TEDQLLEVRNFGDTTLTEVKEKLTELGLYLGMRVPSSMVR"
BASE COUNT         1360 a         3051 c         2891 g         1532 t
ORIGIN      
        1 cctgcaagcc gccggcgccg acatcatcga tgtcggctgc gatccgggcg agccctggcc
       61 gggcgttgcg gactgcgtcc gcgccctcaa agccgaaggg catcgcgtat cgatcgacag
      121 cctcaatccc gccgagatcg ctcccgccgc gggggccggc gcggagctgg tgctgagcgt
      181 taattccacg aatcgccatg cggcgcggga ctggggctgc gaagtcatcg tcattcccga
      241 cgacttcgcc tcgctgggcg gcttggagga aacgctggaa ctgctggccg gcgccggtgt
      301 gcggctgcgg atcgatcccg tgatcgagcc gatcggctgt gggtttgcag cgagcctggg
      361 gcggtatatc gaggtccgcc gccgctatcc ggatgccgag atgctgatgg gcaccggcaa
      421 tctcaccgag ctgaccgacg ccgattccgc cggcatcaat gtgctgctgt tgggcttttg
      481 ccaggagctg ggcgttcgca gcgtgctgac cacccaggtc atcaattggg cccgcacgag
      541 cgtccgcgaa tgcgacctcg cccgccggct ggtctatcac gccgccagcc gccgcatccc
      601 gcccaagcac atcgaagagc ggctaataac gctccgcgat cccaaggtgc acgaattcgg
      661 ggtcgaaacg ctcgagcgcc tcgcgcagca gattcgcgac aacaactacc gcatctatgc
      721 cgaagcgggc gaagtgcatt tgctcggcgc gggcctgcac ctggccgatc gcgacccgct
      781 gctgctcttc gagcggctgc ttcatcccgg tttcggcggc cgtgccgatt cccaccagcc
      841 gccgacgaac gtcgatccgt cgcacgcgtt ctacctcggc tatgaaatgg ccaaggcggc
      901 gatcgcgctg gccctgagca aggagtatcg ccaggacgag gcactcgact ggggctatct
      961 gaccgtcgcg gaaaagagcc accggctgcg gaagtcgaaa gcggcgcgga agtcggcgga
     1021 cgccgcgggc ggcgccgcgg aggacggtgc atgagcgccg ctgcgagcga tcttccgctg
     1081 gtcgtcgagc tgcagccgcc gccggatgtg gaatcggccc tgctgcggct ggcttcgcag
     1141 ccgtacagct tgctgctcga cagtgccttg cgcgatccgc gactggggcg ctactccttt
     1201 ctgagcgcgg atccgtttga gcttctcacg gtgccggctg acggcgcgga cggcctcgcg
     1261 cagcttgcgg agcggctgaa aacctggccg gccggatcgc ggcccgacct gccgccgttc
     1321 cagggcggcg ctgcggggct gttcagctac gacctgtctc gcagtctgga gcggatcgct
     1381 tcgccccggc acgacgaatt cggcgtcccc gcgatggcga tcgggtttta cgacgtggtg
     1441 ctggccttcg atcacgccca gcaccgcgcc tggctgatct cgcaggggct gccggaacgg
     1501 gagccaagcc gccgtttgca gcgggccgcc gcccgcatcg accaattcca gacctggctc
     1561 gctggtgccg cgaccgatcg cccgaatgtc gtcgttcgct ccgcgaacga tcgcccgttc
     1621 gcggagcgaa cggcgacaaa tctcgccccg tcgtttccgg tgcccggccc cgcgggtttg
     1681 cttagcaact tcacggccga cgcctaccgc gcagccgtcc agcgggccat cgactacatc
     1741 tacgccggcg acatcttcca ggtgaacctc tcgcagcggc tgctctgtcc cgccgcgggc
     1801 gacgccctgt cgctctacct ccgcctgcgg cagtgcaatc ccgcaccgtt cgccggctgg
     1861 ttcgatctcg gggagtttca gatcgccagc gcctcgcccg agcggttcct cagcgtccgc
     1921 gaccggcaag tggaagcccg gccgatcaag ggcacgcgcc gacgcaccac ttgggccgaa
     1981 gcggacctct ttgccggtga cgacctgttg cagagcgaaa aggaccgggc cgagaacgta
     2041 atgatcgtcg atctcttgcg gaacgacctg tcgcgggtct gcacgccggg aagcgtccat
     2101 gtcagccagc tttgcggcct ggaaatctat cagttcgtcc agcatctcgt ctcggtcgtc
     2161 cgcggcgagc tcgcaccggg ccgttcgccc gtcgatttga tccgcgcggc gttccccggc
     2221 ggttcgatca ccggcgctcc gaaggtgcgg gcgatggaaa tcatcgccga gctggaaccc
     2281 accgcccgcg ggccgtactg cggcgccctc ggctacattg gcttcgacgg cgcgatggac
     2341 ttgaatatcc tgatccgtac gataacggca ggccgcggct ggtggcagtt tcccgtgggc
     2401 ggagggatcg tggcccaatc gtcgccccag cgcgaatacg aggaaacctg gcacaaagcc
     2461 gagggcctgc tccgggccct tgtgccgatg aaacttgggc cgctcgcatg atcctgctca
     2521 tcgacaatta cgacagcttc gtccacaacc tggcccgcta cctccgccgg atggggcagg
     2581 agacgctggt cgtccgcaat acggagatcg acgtggctgg catcgaggaa ttggccccgc
     2641 aggcggtcgt cctgtctccc gggccctgca ccccgaatga agccgggtgc tcgctcaatg
     2701 tcgtccggtc gctgggagcg agcgtgccga ttctgggcgt ctgcctgggt catcagacga
     2761 ttggcgccgc cctcggcgcg aaggtggtgc gagcgccgca gcccgtgcat ggccgcgcga
     2821 gtctcgttcg tcatgacggc gcgggcgtct tcgccggcct gccgagtccg ctgacggtcg
     2881 gccgatacca ttccttgatc gtcgatcccg ccacgctgcc ggaagacctc gtgccgactg
     2941 cgtggtcgga cgaaggcgtc gtcatggcgc tggcgcaccg ccgctggccg ctcgtcggcg
     3001 tgcagtttca tcccgaatcg attttgactg agtgtggcta cgagctgctg gcgaacttcc
     3061 tgcggctggc cggcttgcac gcggaatcgg cccagcacga aatgaatggt gagcgacgaa
     3121 tcgtcgagca agcctcgccg gctttgcccc tgcaacccgt tacgttctag tgtagccgac
     3181 acgcgcgatc gcgtcgttta acggcgagcc gcaggcgaag ccgttggatg ggtgacgggg
     3241 cgccttcggc tacccgttaa acgaattccc cgtcccgcgt ttctaaggaa cacgacgatg
     3301 ccggccgaac ctgatgagct gatcctggag ggcatcgtag tgacgcagga cgccgccgct
     3361 agcgccaaca tcgccccgat ggggccacgg gtcgatcgcg agcagacgcg cctcgtgctg
     3421 cggccttttc agacggctca gacctttcgc aatctccagg caacccgctg cggcgtcttt
     3481 cacgtgaccg acgatgtcga gctgctcgcc cgcgctgccg tcggccagct cgatccgcca
     3541 tcggcgctgg tgccggtcga tggcttctcg tgtctccggc tcgccgattg ctgccgctgg
     3601 ctggcattcc gcgtgaacgc catcgacgat caaagcgaac gaacgacgat tgactgccag
     3661 gtcattgcca aaggcacgct gcgcgaattc ttcggcttca accgggccaa gcacgcggtg
     3721 gtggaggcgg cgattctcgc tacgcggatc gggatcgtgc ccgatgccga gctgtgggcc
     3781 gaaatggccc ggctgcaatc gccggtcgcg aaaaccgccg gcgagcagga gcgacgggcg
     3841 ttcgagtttc tgcgacaata cgtcgaaagc cgcctcggcc cttcgttccc cgactccgat
     3901 tgacccgcga tcgcccgcat gcccaggctc gtccgcgttg ccgctcccag ccgcctgcac
     3961 ttcggcctgt ggtctttagg cggagagggc cggcagttcg gcggcgtagg ggcgatggtc
     4021 gagcggccgg gcctcgtgct cgaaatcgct ccggctctcg agttcgctgc caccgggccg
     4081 ctcgccgcgc gggccggcgt cttcgcccgc cgctgggcgg agttccatcg ccagccgctg
     4141 cccgcgtgcg ctcttcgcgt ggccgaagcg ccgccggagc atgtcggcct cggcaccgga
     4201 acgcagcttg gcctggccat cgctgcggga atgagcgcct ggtgcggact gccggcgcag
     4261 tcgccgctgg aattggccgt cagcgtcggc cgggcagagc gctcggcgat tggcacgcac
     4321 ggcttcgtgc tgggcgggct gatcgtggag cagggcaagc tgcccgggga gccggtctcg
     4381 ccgctggatt gccgcatcgc cctgcccgag gcgtggcggt tcgtcctcgt gcggccgctc
     4441 ggacaagcgg gcctggccgg cgaggatgaa gcccaggcaa tcgcggcgct gccgccgatc
     4501 tcggacgcgg ttcgcgagcg cctcatctcc gaagcgcgcg acgggctcgt gcctgcggcg
     4561 gcgaccggtg atttccgcac attcgcagcc agcctctacc agtacggcaa tctcgccgga
     4621 caatgcttca aggcccggca gggcggcccg tataacggcc ccgtgctcac cgcgctcgtc
     4681 gagcgcatcc gcagcctggg attcacaggc gtcggccaaa gctcctgggg cccgacgctg
     4741 tttgtcatca gcgagaacga agcatcggcc cgccgtttgg ccgccgaatt agccgccgac
     4801 gcgctcgaga tcctcgtgac cgcgccgtcc aactcagggg ctcggatcga ggtacgcgac
     4861 cccgacccga accccgctaa ccccgctgcc gtacaacgtc catgaattcc cgcctgcgaa
     4921 cgactgcgct ggcttgcgcc ctctcaacgg tgcttcttgg cggctgcccg ttttctgaac
     4981 aatccaacga tccgtccccc gagcccaaag acacacagcc gctgcggctg gttgtcgtgg
     5041 acgatcccgc cttgggagca gcgattgcca gcgagtggca agcccaaacc gagcagtcgc
     5101 tggaaaccga gcagatgacc gcggcagaac tggcccaggc caagctcctg cccggcgaca
     5161 tcgtgctctt tcccaccggc atgctcggcc actgcatcga aggcggcctg attgtcccgt
     5221 tgagcgacga cctgctcgcc gcgccccagt tcgaccgccg cgacatcttc gaccagatcc
     5281 gcctccgcga aatgacctgg ggaaatcgca ccgtcgcggt ctcgctcggc tcgccgcaga
     5341 ttttgctcgc gtatcgggcc gacctgttcg agaagctcgg cctgcagccg ccggccgatt
     5401 gggccgccta ccaagagctc gccctcaagc tcgccgagcg ggccgccctg ggcgaagcgg
     5461 ccccgccgga cgaccagccg tggcacgcca cgctcgagcc gctggccgac ggctgggcgg
     5521 ggcaggtatt gctcgcgcgg gcggctgcct atgcgatgca ccccgaccag ctttcgccgc
     5581 tcttcaattc cgacgatgcc cagccgctga tcgaccgggc gccgtatgtc cgcgcgctca
     5641 aggaactgac ggccgcggcg aaggtcggcg gctttgccga tacgcgcttg acgccgcaag
     5701 aggcccttgc ggaactgcgg gccggacatg cggccatggc gctgggctgg cccgcgccgc
     5761 ctgccggcaa agtcgccgat aaagagacga ccaccggcac gatccgctat gcattgctgc
     5821 ccggcgcaaa acagtcgtac aacttcgcca ccaagcgctg gaccacgagc gaatccggcg
     5881 agccgacgcg cgtgcccgtt ctggcctgct cgggccgcct ggccgccgtc accagcaatt
     5941 ccgcggctcg ccagcgggcg gagagcttcg tgctctggct cggcggcaaa gatgccagcc
     6001 ggcaggtcgc tccccgcagc tccgcgaccg cgccatatcg ccagtcgcag cttcgcgaag
     6061 tcgagctgtg gactgaaggt ctagacccgg gcgaggccag gcaatatggc gtgacgttgg
     6121 ccgctgcctt gagcattccg cgggttttcc cgggcctccg cctgcctggg cggctcgatt
     6181 acgtggctgc actcgacaag gccgtgcagc aagccgcggc tgaacaagcg gcgccggacg
     6241 aggcgctgca gcacgccgcc gcgcgctggg cggaaatcac caacgccatc ggcgccgaca
     6301 agcagcagca ggcgatcctc cgcagcctga gccaggcgga cctgaaataa tcggcttcag
     6361 atatcttccg ccgaaatggc ctggtcgcgc gaaatcacgt ccggtcccgt gccggccgtc
     6421 ttctcatagt ggccgtcgcc ggccttcacg tacttcgtaa atcccatccg gtcgagcttc
     6481 ttgtcgtcgg cgacgttctt ggccatcgcc gagtcggacc attgcccgcc aatcgccggc
     6541 gggcagaaga cgcgccgcac cggctggccg ctctccggat gcttcgtcag cggcggatcg
     6601 gccattttct ggaagatctc aaactgctcg cccggcgagt catcgtcggt gatcagctca
     6661 taaacgtaaa tcggcatggc agtctggaat aacaacccga agcgtaagcg aggggctggg
     6721 atggacacca acccgaagcg taagcgaggg ccctccatct cacttgtact cagcgccccg
     6781 caattcggca actgcgcagc cgcgtggtgc agacattctt gtctgccatt ttcgtcttcc
     6841 ctgtctgctg gcaggcaaga atgcctgccc cactttcact ctactggcat tagccgtggg
     6901 cgggggatgc gaattccgac gatccgctcg tgccgggcga ccagctcgcg gaaatcgctc
     6961 cgcaaaaagc cccacagctc cgccggcggc tgatggccca gccagcggtc ggcaagcaga
     7021 cccatctgct cgtcgtactc ggcctgcgaa tgggtgtggg cgtggtagcg gccggcgtgg
     7081 cggcggatat cgccattgaa cgccgggctg atgtgccaca gctcgtgcag caccgtgacc
     7141 agcttctcgc gcaggtccag gtccaaaaac cgcggcaggc agaaccgcag gatgtatagc
     7201 atttcgcggc cgcttttgtc gcgcaggctc tggatgcggt agctccggcc gcggcgcctg
     7261 ccgacctgcg ccccgtcctg gaaccgcagc ggcgtgagcg atgcaaacag gccgtgccgc
     7321 tcgccgttgc gggcctgcga aaacgccacc gccacgcgcg gcatgtcgat gtgtgccagc
     7381 tccggcagcc gggcgatcat gtcgtcgcag atcagccgca tccgggcggt gatgtccagg
     7441 ggcatcgcga ggtccctccc gcgctcgtag ggtgggctgt gcccaccgct gccagcggcg
     7501 cgacgcggat caccgaacgt ggtgggcaca gcccacccta cgccttcgcc ggctccgcgc
     7561 cttcgtcgct gaaggccggc ttctcgctgc gctgcaccac gcggtcgcgc acgccgacga
     7621 actcgatgat cgcccgcggt ccggcatccc ccagccgcgg cttggcgagc ttgacgacgc
     7681 gggtatagcc ccctttgcgg tcgcggaacc gcggggccag ttcggcaaac agcaccttca
     7741 cggccacctt gtcgcccaag agctgccgag cgcggcggcg ggcggcgacg gcgggcgaga
     7801 tggccttcgc ccagctctgc cacttctcgc tctcgcgcca tttcttccag gcgtcgctgt
     7861 tgcgggcggc gtcggtgccg tgctccttgg cggcttccac gtgctccagc gacttgcagg
     7921 ccagcgtgat gcaccgctcg acgagcggcc gcacttcctt ggccttggcc agcgtggtga
     7981 cgacccgccc cttgactttg ggcgtgttgt cgtcgtcctc ggcgtcgcgc tcggtcagga
     8041 tgagcgcgct ggccagattt ttgagcatgg ccttacggtg cgaaggggct cgccccagta
     8101 cgcggcctcg tttgcggtga cgcatgttct ctccacagaa tcgcatgtcg tagccgcgtt
     8161 cgccagaacg cggggaaccc ccgcactctg gcgagtgcgg ctacaacatt gaacttcgaa
     8221 ttgttgccgg ttaccggacc atcgacgagg ggacgcgcat cccgaggtac aggcccagct
     8281 cggtgagctt ttccttgacc tcggtcagcg tggtgtcgcc gaagttccgc acttccagga
     8341 gctggtcttc ggtcttctgc accaggtcgc ggacggtgtt gatgttttcc gattccaggc
     8401 agttcatcgc ccggaccgag agcttcagct cggccaggct catcgagagc ttggcctcca
     8461 tcgcgccatc gacaccgagc gtcgcgccgc gggccggagc gtgaacgcgc gggcccagct
     8521 ccgcatactg cacgaacggg ttgagatgct tgcggaggat cttggccgct tcgaccaggg
     8581 ccatctccgg atgcaccgag ccgttggtcc acatctcgag cgtgagcttg tcgtagttgg
     8641 tcttctggcc gacgcgggtt tcctcgacct cgtaccgcac acgggtgacg ggactataga
     8701 ccgcgtcgat cggaatgatg ccgatttcgt ggtcccccgt gctgtgctcg gtggccggga
     8761 tgtagccgcg gccgttttcc acgaccattt ccatcatgaa cggcacgttg tcggtcagcg
     8821 tggccagcac gtgg
//
LOCUS       DASUGB010000747         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1888175, whole genome shotgun sequence.
ACCESSION   DASUGB010000747 DASUGB010000000
VERSION     DASUGB010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888175"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFV87_14385"
     CDS             complement(<1..931)
                     /locus_tag="VFV87_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5105003.1"
                     /translation="MGWRVRLTNYAALAVGAWLAGQCAQAQEDVAETPKAKVVIDERP
                     IVERPITQADREHWAFRPIVRPPQPEVQDHAWPRNAVDRFILAKLEAEKIAPAGEAPR
                     TTLLRRLALDLHGLPPSPEEIATFENDTRPDAYERLVDRLLAGPAYGERWAQHWLDLA
                     RFAETDGFEHDKVRPQAWKYRDWVIAAFNADMPYDEFVTWQVAGKSSTERGSRNSELG
                     SSSLPAPSSALPTTFVLAGPDMPDINDQAERRHNLLNEATATAGSVLLGLQMGCAQCH
                     DHKYDPISQADFYRLRAVFEPAVPPLKRDVPVNV"
     gene            complement(1084..1446)
                     /locus_tag="VFV87_14390"
     CDS             complement(1084..1446)
                     /locus_tag="VFV87_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105004.1"
                     /translation="MLWLKSPLAQIAGLSVGMAVLQPLAAAGGANSLLFQFLAAYAMP
                     ITIALWVAADMKAGGHTPPLDLPFFVLLAFPLSLLWYCVWTRGGQGLLLALGLVFLSY
                     LPYVTTAVVWVAWAVALQ"
     gene            complement(1911..>3034)
                     /locus_tag="VFV87_14395"
     CDS             complement(1911..>3034)
                     /locus_tag="VFV87_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105005.1"
                     /translation="ENPLLGPVKPLRPRGQSGLAVSDFLPEIARHADELCVIRSLHGD
                     SVNHPQSVYQMNTGSILMGRPSVGSWVAYGLGSENRDLPAFVVLPDPGGGIKGGPPAW
                     GSGFLPATYQGVTMRPGGQPILHLAPQPQISRQQQRATLDLVLEVNRRHLAARNNDDE
                     LSARIKAYELAFRMQSAAPELVDLKDESAETLALYGLDQKHTREFGQRCLLARRMIER
                     GVRFVQVYSGDTNGWDAHNDVLKNHGQYCQATDLPVAGLLTDLARRGLWNDTLVIWGG
                     EFGRMPMSEQGTGRDHNPWGYTVWLAGGGVKAGIAYGATDPVGLRAAENPVHVHNFHA
                     TLLHLLGLDHEALSFFHNGLDERLIGPTDDVEVVRELLA"
BASE COUNT          538 a         1002 c          935 g          559 t
ORIGIN      
        1 gcacgttcac gggcacgtcc cgcttgagcg gcggcaccgc cggttcgaac accgcccgca
       61 ggcggtaaaa atcggcctgg ctgatcggat cgtacttgtg gtcgtggcac tgggcgcagc
      121 ccatttgcaa tcccagcaac accgagccgg ccgtcgccgt cgcctcgttc agcagattgt
      181 gccgccgctc ggcctgatcg ttgatgtccg gcatgtcggg gccggcgagg acgaacgtgg
      241 tgggaagcgc ggagctcgga gcgggaagcg aggaacttcc aagctccgag ttccgacttc
      301 cgcgctccgt gctcgatttc cccgccacct gccacgtcac aaactcgtcg tacggcatgt
      361 cggcgttgaa ggccgcgatg acccagtcgc ggtatttcca cgcctggggg cgcaccttgt
      421 cgtgctcaaa gccgtcggtt tcggcaaaac gggcgaggtc cagccaatgc tgagcccacc
      481 gctcgccata ggctgggccg gccagcagcc gatcgaccag ccgctcgtag gcgtcggggc
      541 gcgtgtcgtt ctcgaacgtc gcgatttcct ccggcgaggg gggcaggccg tgcaagtcga
      601 gcgccagccg ccgcaagagc gtggtgcgcg gcgcttcgcc ggccggtgcg attttctcgg
      661 cttcgagctt cgcgaggatg aatcgatcga cggcattccg cggccaggcg tgatcctgca
      721 cctccggctg cggcggccgc acaatcggcc ggaacgccca atgctcccga tcggcctggg
      781 tgatcggccg ctccacaatc ggccgctcgt cgatcacgac cttcgcctta ggcgtctcgg
      841 caacatcctc ctgggcctgc gcgcattgac ccgccagcca tgcgccaacc gcgagcgccg
      901 cgtagttcgt tagccgcacg cgccagccca ttcgacatct cgcgaaaggt ttattaccgg
      961 ctcctcttct ctattcattt tcagcggcaa cagggaagag gagcccgtag tccgctttgc
     1021 aaccgatcgg gatcaaccca gcgcccatac tgacattatg ccgccgattc gccgtattgg
     1081 gtctcattgt agcgcaaccg cccaggccac ccatacgacc gcggtcgtta cgtacggcag
     1141 atacgacagg aaaacgagcc ctagcgccaa gagcagacct tgaccgccgc gggtccaaac
     1201 acagtaccac aacagcgaaa gcgggaatgc cagcaggacg aaaaacggca gatccagcgg
     1261 cggtgtgtgg ccgccggctt tcatatcggc ggcgacccac agagcaatcg tgatcggcat
     1321 ggcatacgcc gccagaaact ggaataagag cgagttcgcc ccgccggcgg ccgccagggg
     1381 ctggagcacc gccattccga ccgataagcc ggcaatctgc gccaggggtg atttaagcca
     1441 gagcatttgc ggatgtctca gttgccgcga ctggattcgt cagatttgtc ggccaattgt
     1501 tccttttcac ccggctgtct tgccatgcaa taccgaacat tggcaacgac gcggctaggt
     1561 tcgacgtcgt atcctgtagc acgtggtcca ctccaattca caccgacgtt gaccccgtct
     1621 gattctaatc ctggcgccca aaccgtctcg aactcttccc acgaaagctc atgcggctca
     1681 aaaatggcat aagctggaac agtttttatg atgcgctgaa ctctcgacag actcgaccaa
     1741 aatggctgcg cgcgatcgcc ggtagatgtc tttggttgcg gaaagcccaa ttcatcgcgg
     1801 caagtccata gtcgctggtt cttagcaact tctcgataaa acgcggccgc ttgtgaggct
     1861 gactgaccca tcaatcgacc cttttctccg tgtagccgac gacggcagtt tcacgccaat
     1921 aattcccgca ccacttccac gtcgtccgtc gggccgatca ggcgttcgtc taaaccgttg
     1981 tggaagaagc tcaaggcttc gtggtccagg cccaataggt gcagcagcgt cgcgtggaag
     2041 ttgtggacgt gcactgggtt ctcggccgcg cggaggccga ccggatcggt cgctccgtag
     2101 gcaattcccg ccttcacgcc gccgccggcc agccagaccg tgtagcccca ggggttgtgg
     2161 tcgcggcccg tgccctgctc gctcatcggc atccggccga actcgccccc ccagatcacc
     2221 agcgtgtcgt tccacaggcc gcgccgcgcg aggtcggtga gcaaacccgc caccggcagg
     2281 tccgtcgcct ggcaatactg gccgtggttc ttgagcacgt cgttgtgggc gtcccagccg
     2341 ttggtgtcgc ccgaatagac ttgcacgaac cgtacccccc gctcgatcat tcggcgggcg
     2401 agcaaacacc gctggccgaa ttcgcgggtg tgcttttgat ccaggccata gagcgcgagc
     2461 gtctcggccg attcgtcttt caagtccacc agctccggcg cggccgactg catgcggaaa
     2521 gccagctcat aggctttgat ccgggccgat agttcatcgt cgttgtttct cgccgccagg
     2581 tggcggcggt taacctcaag gaccaggtcg agcgttgccc gctgctgctg gcggctgatc
     2641 tggggctgcg gcgccagatg gaggatcggc tggccgccgg ggcgcatcgt gacgccttga
     2701 taggtcgcgg gcaaaaagcc gctgccccag gcgggcgggc ctcccttgat gccgccgccc
     2761 ggatcgggca ggacgacgaa cgccggcaga tcgcggtttt cgctgcccag gccgtaggcc
     2821 acccaactgc cgacgctggg gcggcccatc aggatgctgc ccgtgttcat ctgatagacc
     2881 gactgcggat ggttcacgct gtcgccgtgc aggctgcgga tcacgcacag ctcgtcggca
     2941 tggcgggcaa tctcgggcag gaagtcgctg accgccaggc ccgactggcc gcgcggccgc
     3001 agcggcttga ccggcccgag cagtggattt tccg
//
LOCUS       DASUGB010000748        12297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2028419, whole genome shotgun sequence.
ACCESSION   DASUGB010000748 DASUGB010000000
VERSION     DASUGB010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028419"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFV87_14400"
     CDS             complement(<1..1023)
                     /locus_tag="VFV87_14400"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5105006.1"
                     /translation="MAEAESSPPASSPGAANPSPADPAPNRLVEACQAASACVTANFR
                     ETVCHHKRHWCIVLAIIVLFVLLGQYLEHQESLLATRYRMYRWVQNPWPRRLAFRDTI
                     VVKIDDDEYWEQASGRVPLRRDYLATLLRKVGAANPAVIGVDVKLPSPNPNGNVKSTA
                     ANGKTLLEDKLYADESAELLDVISELSSSCKIVVAKSLKRAGSDSGTQAKPGHQTVDG
                     HDRAAHPGRPVYSRVADIYDVYGIPLDQVVLAYTFLYPDRRHIPPRLLLDDGSEIDSF
                     ALAAARHFRPELVESKEWIEPRFASFVVRSETTEISAHDLMSATGPDARRIAAALQHK
                     IVLIGGD"
     gene            complement(1381..2343)
                     /locus_tag="VFV87_14405"
     CDS             complement(1381..2343)
                     /locus_tag="VFV87_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105007.1"
                     /translation="MSAQIKSILAGFVVVAAALGGFAWLGMPTSHVFAQAKAPALAAE
                     AKAREANARIAEAEAAEAQARAIAAEAAAAKNPPAPAADAGDATPLDLSKHLRMKAEN
                     FQKITRFPWPAVPTGSQAFAGIPVDIQGALMTFGQRNADNGLKYPETITGIPCGQRFE
                     TLYIMHGTFYEGDPGESAFDVVLNYPGDEQQTITILCGEDTRDWYIKPGEKSRGPTGK
                     RSTLAWTGTGKAGDRDQEIRFCPTAIDNKYPDRLVETIDLVSAKKQTAGCILAISVGK
                     KGLLKPEPENPAENPASGKPALKSALKPNALKVPPPKVKLPASP"
     gene            3112..4464
                     /locus_tag="VFV87_14410"
     CDS             3112..4464
                     /locus_tag="VFV87_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105008.1"
                     /translation="MKKETTNRDRIHESSDRPNSLTTLPVAELIRTPGRSLTPPVLAQ
                     LRAAANQLSARYHNQRQLVWFDQFQPVVNHLNTAKALAESGEIFLDGPAGGLVRVRDN
                     ALERISKGKDLSAVLSDLVDVQYLTDGKLSTAVPASKLDIALRSSVFREQLAVLDGLT
                     TVPAFAPDWSLLRPGYNGDLGDGYRYFLAGEQPRVDRKLKFLPRFLDALRFESDADRT
                     NAFASGLAPYLRLLYPGRLPVNLYLSKSSFGGKGTLFEATCGLCPRVKINLEAADWAN
                     KKNVAIAINADPTTVVVLVDNVDIDLRSAFIESLVTDARQAIHCTGSGDPIGRPYRLV
                     VVVTMNVADVSLDILHRSIPVMLSRPTIVTAWAAQAFRGTGKTVDWRTDDPILDPTVL
                     HRPSSNQRSDNPRTTTYGEQDDPRHPSRQLSGPGQGQDGSAGCRIKTPKQHDSPQLAS
                     "
     gene            4563..5111
                     /locus_tag="VFV87_14415"
     CDS             4563..5111
                     /locus_tag="VFV87_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105009.1"
                     /translation="MKSQRQPSPYHRFPAHCCYFCGSDEHATRHCDLEFCFRCLNPID
                     YEYGCTARKCRCGRRQSPTNVKEILDLICFVVAGNTGRLSRHAVARQMKRVGLFYTAK
                     EISEAFTTNKSNRSIRRLLAAAVPKPQQRLPKEFTSWLNPDPIRQRGSNETVEVLDFA
                     QLAALKDQLSRSAFLDQLRTAS"
     gene            5288..5557
                     /locus_tag="VFV87_14420"
     CDS             5288..5557
                     /locus_tag="VFV87_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105010.1"
                     /translation="MAIISGVPTRTWDKNPLPPDQMAATRDYVNELAKSQRVLTHGLI
                     RPNLGRQELDEMDRQAKELKVCAWKMYTGAEIGEKAWWLDDEEVA"
     gene            5896..6129
                     /locus_tag="VFV87_14425"
     CDS             5896..6129
                     /locus_tag="VFV87_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105011.1"
                     /translation="MIAQRGIRTSGGLPPIRYDAVRTCLEQVSRFAQENAATVHMPRI
                     GCGLAGGTWDEVEPIIVETLVTAGVEATVYDIG"
     gene            6149..6673
                     /locus_tag="VFV87_14430"
     CDS             6149..6673
                     /locus_tag="VFV87_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105012.1"
                     /translation="MDLKPLIHSILTQYALPLNGCHGIAHWARVLENGQRLAKETGAN
                     IEVVTLFAVLHDSQRLSEGGDPQHGPRAAEYALKHRGELFELPDPEFALLHRACEGHT
                     HERTHPDVTIQTCWDADRLDLGRVGVTPHPSRLCTAVAKRPDIIKWADGRACFRVVPA
                     FVIDVWGIDLSSRD"
     gene            6819..7124
                     /locus_tag="VFV87_14435"
     CDS             6819..7124
                     /locus_tag="VFV87_14435"
                     /inference="COORDINATES: protein motif:HMM:NF019248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1580 domain-containing protein"
                     /protein_id="HEX5105013.1"
                     /translation="MHVSTIDPYAIPYTPAGMKQLGLNPPPSPATLHRWRLRGLRGVR
                     LETFLRGGRRFTTRDAVENFFERITAIADRAEPLGYTRRQEDEEIARAMKELEDEGA"
     gene            complement(7207..8238)
                     /locus_tag="VFV87_14440"
     CDS             complement(7207..8238)
                     /locus_tag="VFV87_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX5105014.1"
                     /translation="MSALTGAALPDLALPPPAGQTPPAGQTPPAAVEFHYTQSDSFVA
                     LLQEFGASLLVSTYQANKLLALRAQGQGLSTLVRTFDRPMGLAVDGSRLAVGTRKEVW
                     FLRNAPDIAPQIEPAGMHDACYLPRMSHVTGDIGIHEIAWATPHPRPLSHEGRGEKEL
                     WIVSTRFSCLATLDPDYSFVPRWRPPFITALAAEDRCHLNGLALAEGERGTLVPRYVT
                     ALGTTDVRDGWRAEKPHGGCIIDVASGEFLTRGLSMPHSPRWHGGKLWVLESGKGGLV
                     LVDPSSGKVETVASLPGFTRGLALAGPFAFIGLSKIRPTSAMDGVPLAERRNQLRCGI
                     AVVDDLEAG"
     gene            complement(8273..10966)
                     /locus_tag="VFV87_14445"
     CDS             complement(8273..10966)
                     /locus_tag="VFV87_14445"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5105015.1"
                     /translation="MWFRSMVKALTRFPRRGTQRAANRKQQARRLLLESLEDRRVMAF
                     APAVSYPVGAYPDAVIAADFNNDGRPDLVTANNVGSNVSVLLSNADGTFQPAINSPTG
                     SNPLSLAVGDFDGDGKLDLATANWSDVSVLKGNGSGGFAAPTNINPGSSPSSVAVGDF
                     NSDGKLDLGVITNYYFPGGCGYYACYPGHYFGYANVLLGNGAGSFSLSSTHSLGYGYY
                     TAAASADFNGDGKSDIAASDGYLIAVSLVSGSGVLGAPTYFGGGPNLSSFIAKDATGD
                     GRVDLVTSNFYTNSVSVLAGNGAGSFAAAQSYPAGMQPTSVAVADFNGDSKPDLIAAN
                     REANTVSVLLGTASGTFKPPVNAATGPDSWSLAVGDFNGDGRADAATASNSSGQVFIH
                     LNNAIWPALDAPSISIAFAAAVTEGNTGTVNSNFTVSLSAAYSQPVSVVYSTQDGSAM
                     AGSDYQAVTAATLTFNPGETSKTISIAVFGDRIGEPTEAFSVVLTNPTNGFIANATGG
                     GAILDDEPTVSIESYVVGNEGNTDTKAFTFTVSLSNEYDVPVTVDYTTADLTPDEQWN
                     YGPGATAGTDYAYTSGTVTFPAHTTASQTITVMVNGDRDGESDELFFVNLSNPYGAKL
                     GNWRAMGNIVNDEPYVWIDNYASKPEGNSGTSNLTFMLHLTNAYDLPVTVNYTTHDGS
                     ATAGSDYVAATGSVTIPANTTDAPLDIQIKGDTLVESDEYFSVELTSATNAGLSNNWS
                     YGYILDDDTPPAIYIDDPSVVEGNSGTKLMVFTVYLSQVSGQTVKVNYATANGSAKTS
                     DNDYVAKSGTLTFAPGETSKTISITINGDTRKEQNETLYVNLSGASGATIGDSRGTGT
                     ILNDDAGGKGNGKGNAKLSTAAAFDAALIDLLTPTSKKGGK"
     gene            complement(11110..12015)
                     /locus_tag="VFV87_14450"
     CDS             complement(11110..12015)
                     /locus_tag="VFV87_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105016.1"
                     /translation="MNRREAPHNMQCISARESALCHLRNCQLLVAGHGTAYPSAQFRG
                     RPQGDFIVENCLIGQQLLFYVTHDQPMRAALNHNTVLELFLTIRHHHQLDLAARQPGA
                     GEFQIETSSNILGGPLMFVQPQEFVGSEVEFSGQACERVLRQVVTWRENGNLYSSSDG
                     SELLRLFLEPGNAPPRQRLALETPLNDLPQWKNFWALESLNSLRGEAKFQGGSLVAKA
                     KFDPEALTPDLFRLRPDSPGYRAGSDGKDLGADVDLVGPGAAYERWKRTPEYKQWLKE
                     TRQVKSQESGVGDLEPASDAKPTDS"
     gene            11992..>12297
                     /locus_tag="VFV87_14455"
     CDS             11992..>12297
                     /locus_tag="VFV87_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105017.1"
                     /translation="MGRFAAVHQEPAIDRIQPCLRAAGSDQGATRTAGLRVDPPQIQT
                     LQRQKGLTVEQTIRLLVRIGHNDRRIAFARTDLEGLLNDQDGFRNERPIAAYFNGVAV
                     "
BASE COUNT         2371 a         3677 c         3825 g         2424 t
ORIGIN      
        1 atcgcccccg atcagcacga ttttgtgctg cagtgcagcg gcgatccgcc tggcatcggg
       61 cccggtggcg ctcatcaagt cgtgggccga gatctcggtt gtttccgagc ggaccacgaa
      121 gctggcgaat ctcggttcga tccattcctt cgactcaacg agctccgggc ggaagtgccg
      181 ggccgcagcc aaagcgaacg agtcgatttc actcccatcg tcgagcagca gccgcggtgg
      241 aatgtggcgg cggtcgggat acaagaacgt ataggccaga acgacctgat cgagcggaat
      301 gccatacacg tcgtaaatgt cagccacgcg gctataaacc ggccgaccgg gatgcgctgc
      361 gcgatcgtgt ccgtcaacgg tctggtgacc cggcttcgcc tgggttcccg aatcagatcc
      421 ggctcgcttc aggctcttcg caacgacgat cttgcacgaa ctggaaagct cgctgatgac
      481 gtcgagcagt tcggccgact cgtcggcata gagcttgtct tcgagcaatg tctttccgtt
      541 ggcggccgtc gatttgacgt tcccgtttgg attgggcgac ggaagtttca cgtcgacgcc
      601 gatcacggcg gggttcgcgg cgccgacttt ccttagcaga gtcgcgagat agtcgcggcg
      661 aagcggcacg cgtcccgacg cttgttccca gtattcgtcg tcgtcgatct tgacaacgat
      721 ggtgtcgcga aaggccaacc gccgcggcca ggggttttga acccatcgat acatgcgata
      781 acgcgttgcc agcaggctct cctggtgctc cagatactgc ccgagcagca cgaagagcac
      841 gatgatcgcg agaacgatgc accaatggcg cttgtggtgg caaactgtct ctcggaagtt
      901 ggctgtgaca caggcagacg ccgcctggca ggcttcgacc aggcgattcg gcgcgggatc
      961 agcgggcgaa gggttggcgg cacccggaga gctggcgggc gggctggatt ctgcttctgc
     1021 cactagggat tccccctgaa gcgtgggccg agaggcatgc taacggtggt aattgcggat
     1081 tgcaagcatt tgccaagcta gtccctccag gcgagatcgt cctgccggcc cggccgaacc
     1141 ccaatcttca tcactcctgt ggcgacaatg ggcggagtgg cacgaaggca agtggcgacg
     1201 ccgcagcaag tcggaagatc ggccgtggcg catttcgcgc ccgaagcgga ggccgggcgt
     1261 tacgcatacc aaatgcaaga cgtagcgatc cgatggactg cggcgccagc ggccggcttt
     1321 ggacggcgcg gccgagtgca gttgatccat gggagaagtg ccggtccagc gattgctaaa
     1381 tcaggggctg gcaggcagtt tgacctttgg cggaggaacc tttagcgcat tcggctttag
     1441 cgcacttttg agggcgggct tgccggacgc cgggttttcg gcgggatttt ccggctccgg
     1501 cttgagcagg cctttcttgc cgacggagat ggcgaggatg cagccggcgg tttgcttttt
     1561 ggcggagaca aggtcgatgg tttcgaccag gcggtcgggg tacttgttgt cgatggcggt
     1621 ggggcagaag cggatttcct ggtcgcggtc gccggccttg ccggtaccgg tccaggcgag
     1681 tgtggagcgc ttgcccgtgg ggccacggct tttttcgccc ggtttgatgt accagtcgcg
     1741 agtgtcctcg ccgcagagga tggtgatggt ctgttgctca tcgcccggat agttgagcac
     1801 gacgtcgaac gccgactcgc ccggatcgcc ctcgtagaaa gtgccatgca tgatgtacag
     1861 agtctcgaac ctttgcccgc aaggtatgcc ggtgattgtc tcggggtatt tcaagccgtt
     1921 gtcggcgttg cgctggccga aggtcatgag cgcgccttga atgtcgacag gaatgccggc
     1981 gaaggcttgc gacccggtgg gcacggcggg ccagggaaac cgcgtgatct tctgaaagtt
     2041 ctcggctttc atccgcaggt gcttcgagag gtcaaggggc gtcgcgtcgc ccgcatcggc
     2101 cgccggggcc ggcgggtttt tggccgcagc agcctcggcg gcaatggcgc gggcctgggc
     2161 ttcggcggct tccgcctcgg cgatcctggc gttggcctcg cgggctttgg cttccgctgc
     2221 gagggcgggc gccttggctt gggcaaacac gtgcgaagtc ggcattccaa gccaggcaaa
     2281 tccgcctagc gccgcagcca cgacgacgaa tccggccagg atcgatttga tttgagcgga
     2341 catgtgcgaa ctcccgtgca agaccccagg gacgagcaga aaaagacatt atgtcttgtt
     2401 tctggccact cgtccagcgg aggcggcgcg ggcgccatgc acgccttccg caagaaacta
     2461 aaagagccgc ccgacgtaag tcgcttgcgg ctcaaggtac acccagcagg actcgaacct
     2521 gcaaccttcg gttccgtagt taacggagcc gcggccgccg ttatcgctgc aaacgtaaat
     2581 ggttaatgaa gttagtggcg attgcgatcg ctcgcagttc cttgaggcaa tcgccgtaag
     2641 tccttacgcg tcggtggtcg aaatcagcaa aagtggtagt agcgggtagt actcgaagca
     2701 cgtcgccacc gactcttgcc tttcgcaagc gattgcctat tcttaaaccc tgcagggaca
     2761 acttgcaaga cacacggctc gccaggtcaa ccgatcttgg cggcacatgc ggccgaccgg
     2821 acagtccgaa tcagcgggtc cgcggtcgct taaacgaacg actgagacaa tccgtgagtt
     2881 ggtgggtggc aatctttcct tagccgcctg gcgaatcgca ctgcgaaccg ccggtaacca
     2941 accccgcggc cggtagtccg cttcacccga ccaaccttgg tcggccgtgc gggctctctt
     3001 ccttttcccc cgcagttcca ttcgcttggc ccgcaagtgc cattggcata attgcaagcc
     3061 agcgaatgac tcggcggtca cgtatctgca tttttccaag ggcatgaatc catgaagaaa
     3121 gagaccacca accgcgatcg catccacgaa tcttctgacc ggccgaattc gctcaccacg
     3181 ctgccagtag cggagctgat tcgtactcca ggcaggagcc tgacgcctcc cgtcctggcc
     3241 caactaaggg ctgcggcgaa tcaactttca gcccgctacc acaaccagcg tcaactcgtt
     3301 tggtttgacc aattccaacc tgtcgtcaat cacctcaata ccgcgaaggc tctcgctgaa
     3361 tcgggagaga tcttcttgga cggtccggcg ggagggctag tacgcgtgcg agacaacgca
     3421 ctcgaacgca tctcgaaagg aaaagacctg tcggcagtgc tgagcgatct cgtcgatgtc
     3481 cagtatctga ccgatggaaa actttcaact gcggtgccag catcgaaact ggacatcgcc
     3541 ttgcggtcga gcgtgtttcg tgagcaactt gccgtgctgg acggcctaac gaccgtcccg
     3601 gccttcgcac cggactggtc gctcctacga cctggataca atggcgatct gggtgacggc
     3661 tatcgttatt ttctggcagg cgaacaaccg cgagttgacc gcaagctcaa gttccttcca
     3721 cggtttctcg atgcccttcg gtttgagtca gacgccgatc gaaccaacgc atttgcgtct
     3781 ggcttagcac cctatctgcg attgctctat ccgggtcggc tgcccgtcaa cctctacctg
     3841 tcgaagagtt cattcggcgg caagggaacg ctctttgagg caacgtgcgg tttgtgcccc
     3901 cgcgtaaaga ttaacctgga ggcagcggac tgggcgaaca agaaaaacgt cgccatcgcg
     3961 atcaacgccg acccgacgac cgtcgtggtg ttagtcgaca atgtcgacat cgaccttcgc
     4021 tccgcgttca tcgagagtct ggttaccgac gcccgccagg ccatccactg cacgggatct
     4081 ggcgatccga ttggccgtcc gtatcggctc gtagttgtcg taacgatgaa tgtggccgac
     4141 gtgtcactgg acatcttgca tcgttcgatt cccgtgatgc tcagccgccc gacaatcgtg
     4201 acagcgtggg ccgctcaagc gtttcgcggt acgggcaaaa ccgttgactg gagaacagac
     4261 gaccctatac ttgatcccac ggtgctgcat cgaccaagca gcaaccagag gtccgacaac
     4321 cctcgaacca caacatacgg cgagcaggac gacccccgcc atccatcgcg acagctgagc
     4381 ggccccggtc agggccaaga cggatcagct ggctgccgca tcaagacgcc gaagcaacac
     4441 gatagcccgc aattagcgag ctaactgtgc tcgcttcctt cgaggccacg ctcgttgccc
     4501 gccattgtgc gtgcgacccc tgtcgtgacg cggatcgaac cattttgttt cggagtatcg
     4561 ctatgaaatc ccaacgccag ccatcaccgt accaccgatt ccccgctcac tgctgttact
     4621 tctgcggcag cgacgaacat gccactcgac actgtgacct ggagttctgt ttccgctgtc
     4681 tgaatccgat cgactacgaa tacggctgta ccgcccgcaa gtgccgatgc gggcgacgcc
     4741 agagtccgac caacgtgaaa gagatacttg acctgatctg cttcgtcgtg gcgggtaaca
     4801 ctggaaggtt gagtcgacat gctgtcgctc ggcaaatgaa acgggtcgga ctcttctaca
     4861 ccgcaaagga gatctccgag gcgttcacaa ccaacaagtc caatcgaagc atccggcggc
     4921 tacttgccgc agcagttcca aagccgcagc aacggctgcc gaaggaattc acgagctggt
     4981 tgaatcctga tccgatccgg caacgcgggt cgaatgagac ggtcgaagta ttggatttcg
     5041 cacagctggc cgcgttgaag gatcagctca gccgatccgc attcctcgat cagctccgga
     5101 cggcgagtta gctcgttttc tgcggtggtc gctgccacct cgcaactgca tccagcgaag
     5161 ggataaacgt ttatagaaac ccgaccgaag gcaatctagc tggacggccg ccacgatcga
     5221 cctcagcgtc gaacggctca accgcgatca ctacgtcaaa gaggtgttct tcgacagcga
     5281 caccgtcatg gcaatcatca gcggcgttcc gacgcgtacc tgggacaaga acccgctgcc
     5341 gcccgatcaa atggctgcga cgcgggacta cgtcaacgaa ttggccaaat cgcagcgagt
     5401 cctgacccac ggcttgatcc gaccgaacct gggaaggcag gaactcgacg agatggaccg
     5461 ccaggcgaaa gagttgaaag tctgtgcctg gaagatgtac acgggggcgg aaatcggcga
     5521 gaaagcctgg tggctcgacg acgaggaggt cgcttaagcg tgattgtcat cgacggatat
     5581 gtcactcttg gcgacaagcc gggcctggga atgagactgt gggatacgat cggtcaggca
     5641 actcccatca ttggagttgc gaagactcga tttgaagggg cagttgcgtc cgaagtattc
     5701 cgcgggcaaa gtgaactgcc ccttcatgtg acggtggcag gaatcgcgtt atccgaggcc
     5761 acagcaagcg tgagcacaat ggccggtgac ttccgcattc cgacactgct taagcgggtc
     5821 gatcggctcg caagagacga acttggtcga attctgtgtc gtcgaactac atcgtagcgt
     5881 cgttgggttg ccaacatgat cgcccagcga ggcatccgca cgagcggagg tcttccgccg
     5941 atccgctatg acgccgtgcg aacgtgcctg gagcaagtct cgcgttttgc tcaggagaat
     6001 gccgccactg ttcacatgcc gcgaattggt tgtggattgg cgggaggaac ctgggatgag
     6061 gtggaaccga ttatcgtgga aaccctcgtc acggcgggcg tcgaggcgac ggtttacgat
     6121 attggttagc tgaagtaaca ggtgtgacgt ggacctcaag ccactgattc actcaattct
     6181 cacccagtac gccttgccat tgaacggctg ccacgggatt gctcattggg ctcgtgtgct
     6241 ggagaacggc cagcgactgg cgaaagagac cggagcgaac atcgaggtcg tcactctatt
     6301 cgcggtgctg cacgactccc agcggctaag tgaaggtgga gatccccagc atggcccgcg
     6361 tgctgcagag tacgcgttga aacaccgcgg cgagcttttt gagctgcccg atcccgagtt
     6421 tgcattgctc caccgggcct gtgagggaca cacgcatgag cgaactcatc cagacgtgac
     6481 aatccaaact tgctgggatg ctgatcggct cgacctgggc cgcgtcggcg tcacgcctca
     6541 cccgagccgg ctctgtacgg cagtagccaa gcgacccgac atcatcaaat gggctgacgg
     6601 gagagcgtgc tttcgagtcg tgcctgcatt cgtaatcgac gtttggggca tcgacctatc
     6661 atcacgcgac tagtctaggc gtgaccagca agacgtcgga acgttcaaca cgttcaacac
     6721 gttttcttca gtccgaagcg ttgtcttcaa cactcaattg cggtccctgc ggcagtgaca
     6781 tgtgaggcaa gtccgccctc gttacgccag gtttcgcaat gcacgtttcc accatcgacc
     6841 cctacgcaat tccctatacg cccgccggga tgaagcagct cggtctcaac ccaccaccca
     6901 gtccagcaac gctccaccgt tggcggctac gaggactgcg tggtgtgagg ctggagacat
     6961 ttctccgcgg aggacggcga tttaccacga gagatgccgt cgagaacttc ttcgagcgga
     7021 tcacggccat tgcggatcga gcggaaccgc tcggttacac tcgccgacag gaggacgagg
     7081 aaattgcccg agcaatgaag gagcttgaag acgaaggagc ctagctggct ctggaatcgg
     7141 acgaagtcgc gattgagcca gaaaacagaa ctgccccgcc ctggtccgtg aggctcaaca
     7201 actgttctag cctgcctcaa ggtcgtcaac taccgcgatg ccgcaccgga gctgattgcg
     7261 gcgctcggcc aagggcacgc cgtccatcgc ggaagtcgga cggatcttgg agaggccgat
     7321 gaaggcgaag ggaccggcaa gggccaggcc gcgcgtgaag cccgggagcg aggccacggt
     7381 ttcaaccttc ccgctgctgg gatcgacgag gaccaggccc cctttcccgg attccagcac
     7441 ccacagcttg ccgccgtgcc accggggcga atggggcatc gagaggccgc gcgtcaagaa
     7501 ttccccgctg gccacgtcga tgatgcagcc accgtgcggc ttttcggccc gccagccatc
     7561 gcggacgtcg gtcgtgccga gcgccgtgac ataccggggg actaacgtcc cccgctcgcc
     7621 ttcggcgagt gccaggccgt tcaagtgaca gcgatcctcc gcggccagtg cggtgatgaa
     7681 cggaggccgc cagcgtggga cgaagctgta atcaggatcg agcgtcgcca ggcatgagaa
     7741 ccgtgtgctg acgatccaca attccttctc ccctcgcccc tcgtgggaga ggggccgggg
     7801 gtgaggggtt gcccaggcga tctcgtgaat gccaatgtcg cccgtgacgt ggctcatccg
     7861 cggcagatag caagcatcgt gcattcctgc cggctcgatc tgcggtgcaa tatcaggcgc
     7921 gttccgcaag aaccacacct ccttgcgagt gccgacggcc agccgggatc cgtccacggc
     7981 cagccccatc ggcctgtcga acgtgcggac cagtgtggac aatccctgtc cctgggcgcg
     8041 cagcgcgagc agcttattcg cctgatacgt gctgacgagc agcgacgcgc cgaactcctg
     8101 cagcagggcg acaaagctgt cactctgcgt gtaatggaac tcgaccgccg ccggcggcgt
     8161 ttggccggcc ggcggcgttt ggccggccgg cggcggcagt gcgaggtcag gcagcgcggc
     8221 gcccgtgagt gctgacatgg attgtccctt cgtggcgttc ggactgagca gcctacttgc
     8281 cccctttctt ggaagtcggc gtcaacaagt cgatcagtgc ggcatcgaac gcggcggccg
     8341 tcgagagctt cgcattgccc ttcccgttgc ccttgccgcc tgcatcgtcg ttcaggatgg
     8401 tccctgtgcc gcggctgtcg ccgattgtgg cgccgctggc gccagagagg ttcacataga
     8461 gcgtttcgtt ttgctctttg cgggtgtcgc cgttgatcgt gatcgaaatc gtcttgctgg
     8521 tctcgcccgg tgcaaaggtc agcgtgccgg acttcgccac gtagtcgttg tcggaggtct
     8581 tggccgagcc gttggcggtg gcgtagttca ccttcactgt ttgcccactg acctgtgaca
     8641 gataaaccgt gaagaccatc agcttcgtgc cgctgttgcc ctccaccacg gacgggtcgt
     8701 cgatgtagat cgccggcgga gtgtcgtcat cgagaatgta gccgtagctc caattattgc
     8761 tgagaccggc gttcgtagcg ctcgtcagtt cgacggagaa atactcgtcc gactcgacca
     8821 acgtgtcgcc cttgatctgg atgtcgagcg gcgcatcggt cgtgttggcg ggaatcgtca
     8881 cagatcccgt cgccgccaca tagtcgctgc cggcagtcgc cgagccgtcg tgggtcgtgt
     8941 agttcaccgt cacgggcaag tcgtaggcgt tcgtcaaatg cagcatgaag gtcaggttcg
     9001 acgtgcccga gttgccctcc ggcttgctgg cgtagttgtc gatccagacg tagggctcgt
     9061 cgttcacgat gttccccatg gctcgccaat tgcctagttt ggcgccgtaa gggttgctga
     9121 ggttgacgaa gaacaattcg tccgactcgc cgtcgcgatc gccgttgacc atcacggtaa
     9181 tggtctggct ggccgtcgtg tgggccggga aggtcacggt tcccgacgtg taggcatagt
     9241 cggtgccggc ggtggcgcct ggcccatagt tccactgctc gtcgggggtg agatcggcgg
     9301 tggtataatc aaccgtcacg gggacgtcat attcgttcga gagcgacacg gtgaacgtaa
     9361 aggctttggt gtcggtgttg ccctcgttgc cgacgacgta actctcgatg ctcaccgtcg
     9421 gctcgtcgtc caggatcgcg cccccgccgg tggcattggc gatgaagcca ttcgtcggat
     9481 tcgtgagtac gacggaaaac gcctcggtgg gctcgccaat ccggtcgccg aaaacggcga
     9541 tgctgatcgt cttgctggtc tcaccaggat tgaatgtcag cgtcgccgcc gtcaccgcct
     9601 gatagtcgct gccggccatt gcgctgccgt cctgcgtgga atagacgacg ctcaccggct
     9661 ggctgtaggc ggccgacagg ctgacggtga aattggagtt gaccgtgccg gtgtttccct
     9721 cggtgacggc cgccgcaaac gcgatcgaga tcgaaggggc gtcgagcgcg ggccagatcg
     9781 cgttgttgag gtggatgaag acctggccgc tggagttgct ggccgtcgcc gcatcggcgc
     9841 ggccgtcgcc attgaagtcg ccgaccgcga ggctccacga atccggcccc gtggcggcgt
     9901 tgactggcgg cttgaacgtg ccgctggcgg tgcccaggag cacgctgacc gtgtttgctt
     9961 cccggttggc tgcgatcagg tcgggcttgc tgtcgccatt gaagtcggcg acagctactg
    10021 acgtgggctg catgcccgct ggataggact gggccgcggc gaaggagccc gcgccgttgc
    10081 ccgcgagcac gctgaccgaa ttcgtgtaga aattggacgt gacgaggtcc actcgcccgt
    10141 cgcccgtggc gtccttggcg atgaacgaag acaggttcgg tcctccgccg aagtaggttg
    10201 gcgctccaag aacaccgctg ccggagacaa gcgagacggc aatcaaatag ccatcgctgg
    10261 ccgcgatgtc cgatttgccg tcgccgttga aatccgcgct ggctgcagcc gtgtaataac
    10321 cgtagccgag ggaatgtgtg ctggataacg agaacgagcc cgcgccattg ccgaggagca
    10381 cgttggcata gccaaagtaa tggccaggat agcaggcgta atacccgcag ccgccgggga
    10441 aatagtaatt cgtaatcaca cccagatcga gcttgccgtc gctattgaag tcacccacgg
    10501 cgaccgagga cggactcgaa ccagggttaa tgttcgtcgg agcggcaaaa ccgccgctgc
    10561 cgttgccctt gagcacactt acgtcgctcc aattggcggt ggccaggtca agcttgccgt
    10621 ctccgtcgaa gtcgccgacg gcgagcgaaa gcgggttcga tccggtgggc gagttgatgg
    10681 ccggttgaaa tgtgccatcg gcattgctca gcaggacgct gacgttgctg ccgacgttgt
    10741 tggccgtgac caggtcgggc cggccatcgt tgttgaagtc ggcggcaatc accgcgtcgg
    10801 ggtaggcgcc cacgggatag ctgacggctg gggcaaacgc catcacacgg cggtcctcca
    10861 ggctttccag cagaagccgc cgcgcctgct gtttgcgatt ggcggcacgc tgcgtgccgc
    10921 gccgcgggaa acgagtcaac gccttgacca tcgaacgaaa ccacatggga aatctccaat
    10981 tcacgtgtgg cctgcgctat aatggcggcg gcgagccacc cagcgcgcag accgtgcgcc
    11041 gggcggtgcc gggccggtga ggagctttct tgacggttag ggcctcaccg gccgctttgt
    11101 tttcagaagt caggagtcag ttggcttggc gtcgcttgct ggctccagat ccccgactcc
    11161 tgactcctgc gatttcactt gtcttgtctc tttcaaccac tgcttatact ccggcgttct
    11221 cttccagcgc tcgtaagccg caccggggcc gaccaggtcc acgtcggcgc cgaggtcctt
    11281 gccatcagaa cccgcgcggt agccggggct gtccggacgt aggcggaata ggtcgggcgt
    11341 gagcgcctcg ggatcgaact tggccttggc gaccaggctc ccaccctgaa acttcgcctc
    11401 gccgcgcagg gaattgaggc tctccaaggc ccaaaagttc ttccactgag gtaagtcatt
    11461 caatggcgtt tcgagggcca accgctgacg cggcggggcg ttacccggct ccaaaaacag
    11521 cctgagcagt tcgctgccgt cggacgacga gtagaggttt ccattctctc gccaggttac
    11581 gacttgtctt aaaactcgtt cgcaggcctg gccactgaac tcgacttcac ttccaacaaa
    11641 ctcttgcggc tgcacgaaca tcagcgggcc gccgaggatg ttcgacgaag tctcaatttg
    11701 aaactctccc gcacccggct ggcgtgccgc gagatcgagc tggtgatgat gtcggatggt
    11761 caagaataat tccaaaaccg tgttgtgatt cagcgccgcc cgcatcggtt ggtcatgggt
    11821 cacatagaaa agtagttgtt gcccgatcag gcaattctcg acgatgaaat cgccctgggg
    11881 cctgcctctg aattgggcgc tgggatatgc cgttccgtgg cctgccacca acagctgaca
    11941 gtttcggagg tgacatagcg ccgactcgcg ggcgctaatg cattgcatat tgtggggcgc
    12001 ttcgcggcgg ttcatcagga accggcaatt gaccgcatac agccgtgcct cagagccgct
    12061 ggaagcgacc agggagcgac ccggaccgcc gggcttcgcg ttgacccacc gcaaatccaa
    12121 accctccagc gccagaaggg cctcactgta gagcaaacaa tccgcctgct cgtccggatc
    12181 ggccataatg accggcgaat agccttcgcc cgcacggatc ttgagggact gctgaacgat
    12241 caggatgggt ttcgtaacga acggcccatt gccgcgtatt tcaatggcgt cgccgtt
//
LOCUS       DASUGB010000749         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2028686, whole genome shotgun sequence.
ACCESSION   DASUGB010000749 DASUGB010000000
VERSION     DASUGB010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028686"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFV87_14460"
     CDS             complement(<1..297)
                     /locus_tag="VFV87_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEX5105018.1"
                     /translation="MQILVFEDDAPRLYPLTLGRAAYAISCASYRLVDWLGLLAEEAG
                     ATLGGLVRPHLRAIQKLDFPQFRGQGATSELPALMVNARVAPTVAAYRILQKLID"
     gene            complement(489..830)
                     /locus_tag="VFV87_14465"
     CDS             complement(489..830)
                     /locus_tag="VFV87_14465"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5105019.1"
                     /translation="MAYENLTEEQLKTQLAELPGWEVRDGWLRRTFHTPGWPHTLMLA
                     QTIAYLAEAGYHHPDLKLGYATCTVLLQTHKVKGLTMHDIELAKKITEVALWQPPPGS
                     ALEGNPKKWVH"
     gene            complement(869..1837)
                     /locus_tag="VFV87_14470"
     CDS             complement(869..1837)
                     /locus_tag="VFV87_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105020.1"
                     /translation="MGSFNHNFHIQSGDSDRVVKAIRDVLAKEGWRASDKPPVEESQR
                     FGQSASGRALWVSKPAAGWTSILDSDLAGSMSLAPALAAELDAYALLCLVNDSDSWRY
                     TLAHGKELLDEFDSAAASGPSEYSAAKLGQIAANLGLWQQQMADGTLQRRVAELQERF
                     IESAPPAIKEIRDQIAGGQVAPESLAQYHAWMLSQAPRMTALVQEVMGDLLGAPPVPE
                     SVEAGKSKRRTRKKAKAQRAALREHIERLRPLLAAAVTDDQLEAVLDEKATFAEETLA
                     RFLPLVGLQTFYAYLDYDHKDEASQDELAAHGIEFAHHLMFELPGP"
BASE COUNT          354 a          641 c          629 g          400 t
ORIGIN      
        1 gtcgatcagc ttctgcagga tgcggtaggc ggcgaccgtc ggcgcgacgc gggcattgac
       61 cataagcgcg ggcaactcgc tcgtggcccc ctggccgcgg aactggggaa aatccagctt
      121 ttggatcgca cgcaggtgcg gccgcacgag cccccccagc gtcgccccgg cttcctcggc
      181 cagcaaaccg agccagtcca ccagccggta gctggcgcag ctaatcgcgt acgcggcccg
      241 tccgagcgtg agcgggtaga ggcgcggggc gtcgtcttcg aagaccagga tctgcattgc
      301 ggactgcgga ctgcggatta ccggattaca gattactcag aagtttagcg gaaggctgta
      361 cgcaatgaac gggccgatgc cctgtccgcc gatcgccggc ctgcgaaaag aaggctggtt
      421 gagctaacgc cacctacgct catttaagcc gtcaatgcaa ccgaccctgg gccagccaca
      481 cgagtgcgtt aatgcaccca ttttttggga ttgccttcga gggccgaacc ggggggcggc
      541 tgccagaggg caacttcggt gatttttttg gccagctcga tgtcgtgcat cgtcagccct
      601 ttcaccttgt gcgtttgcaa cagcaccgtg caggtggcgt agccgagctt gaggtcgggg
      661 tggtgatagc ccgcttcggc caggtaggcg atcgtttggg ccagcatcag ggtgtgcggc
      721 cagccggggg tgtggaacgt gcggcggagc cagccgtcgc ggacttccca gcccggtagc
      781 tcggcgagct gggtcttcaa ttgctcctcg gtcagatttt cgtatgccat ggcagcacct
      841 cccaaggcca ggtggcgaat cgtttccatt acgggccggg cagttcaaac atcaagtggt
      901 gtgcaaactc aatgccgtgc gctgccaatt cgtcctgact tgcttcgtct ttgtgatcgt
      961 aatcgagata ggcgtagaac gtctgcagcc cgaccagtgg aaggaacctg gccaacgtct
     1021 cctcggcgaa tgtggctttc tcgtccagca ctgcttcgag ctggtcgtcg gttacagcgg
     1081 cagcgagcag cggacgcaac ctttcgatgt gttcgcgcag cgcggcgcgc tgcgccttcg
     1141 ctttttttcg cgttcgccgt ttcgactttc ccgcttcgac ggactccgga accggcggcg
     1201 cgcccagcag atcgcccatg acttcctgaa caagagccgt catgcgcgga gcctggctca
     1261 gcatccaggc gtggtattgg gccagcgact cgggagcaac ctgtccgccg gcaatctgat
     1321 cgcgaatctc cttgattgcg ggcggtgcgg actcgatgaa acgctcctgc aattcagcca
     1381 cccgccgttg cagggtgccg tcggccattt gctgttgcca caaaccaaga ttcgccgcga
     1441 tctgaccgag cttcgcggct gagtactcgc taggcccgct cgccgctgcg gaatcgaact
     1501 cgtcgagcag ctccttgccg tgcgcgaggg tatatcgcca tgaatcgcta tcattgacca
     1561 ggcacagcag ggcgtacgca tcaagctccg ctgccagcgc cggcgcaagc gacatcgagc
     1621 cggcaagatc gctgtcgagg atgctggtcc atcccgcggc gggcttggat acccagagcg
     1681 cacggccgct ggccgactgg ccaaagcgct gcgattcttc aacgggcggt ttatccgacg
     1741 cacgccatcc ctccttcgcg agcacgtcgc ggattgcctt gacaacccgg tctgaatcgc
     1801 cggactggat gtgaaagttg tggttgaaac tccccactgc gtgttctccg ccgcctggtc
     1861 gagcgcgctc gtacgtgcgg ttgagcgatc gcgcttcggt ccgtacaatt ctccgctccc
     1921 cagcgccgat tgcaattcga ccaccgccaa ctgccactcg cggagtagcc agacatggaa
     1981 cgcactctcg tcctcctgaa acccgactgc gtgcagcggc ggct
//
LOCUS       DASUGB010000750         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_223252, whole genome shotgun sequence.
ACCESSION   DASUGB010000750 DASUGB010000000
VERSION     DASUGB010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223252"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFV87_14475"
     CDS             <1..254
                     /locus_tag="VFV87_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105021.1"
                     /translation="PPSPRWQARAKGRQLAVSPAHEDFAALLAEALDTLAACDWDVGV
                     AAKRLNVTASQLVKLLQREPPALGQLNDQRRLRQLVPLK"
     gene            364..987
                     /locus_tag="VFV87_14480"
     CDS             364..987
                     /locus_tag="VFV87_14480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5105022.1"
                     /translation="MSNFDRQKWNAKYQTEPAPREPSQGLIALDCFLPRSGKAIDLAC
                     GAARHGIWLAQRGLDVTIADISAVGLSLAMERAAERGVSIKPLEIDLEHDSFPPGPWD
                     LILSVCYLQRSMFAAYPTALAPGGTLVVIQPTKKNLERHAKPPADFLLDEGELPGLIR
                     SLEVLHYQEAWSADDRHDAVLVARRPVGQAFLPAGQTRVSAPQSGIV"
     gene            1006..>2540
                     /locus_tag="VFV87_14485"
     CDS             1006..>2540
                     /locus_tag="VFV87_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5105023.1"
                     /translation="MKLLRVGAAVLNQTPLDWDGNLARIRGAIAAAKAGGVSLLCLPE
                     LCITGYGCEDAFHAPGTQSTAIDILCELRGETRDMAVSFGLPLLVNGSLYNTACLVVN
                     GQILGFVGKQNLAGEGIHYEPRWFKPWPQGQVTGVKIRDIAHDVGDLLFDVGGINVGF
                     EICEDAWVGDRPGVSLARRGVDVIFNPSASHFAFGKHEIRKRFVTEGSRAFSVGYVYA
                     NLLGNESGRAIYDGDAMIAAAGRLVVTGPRFSYADFYVTHAVIDIDAIRMSRARNASF
                     RPIIQIPTRDYIGGDFTFPKLRPVKETATHPAWESSPHIKEEEFTRAEALALFDYLRK
                     SRSHGFVVSLSGGADSAAVSYLAAKLVDFAVAEIGREAFLKKLSYIPGLAQATDNRAF
                     VRRLLSCVYQPTRNSGEVTRNAAAAVAKALGAEYLEFNVDELVQGYTSVVSQALGRKL
                     DWKTDDLALQNIQARARAPSVWMLANIKRALLLATSNRSEAAVGYVTMDGDSSGGLSP
                     IAG"
BASE COUNT          452 a          835 c          779 g          474 t
ORIGIN      
        1 agccgcctag tccgcgctgg caagcgcgtg ccaaggggcg tcaactcgcc gtcagtcccg
       61 cgcacgaaga cttcgccgct ctcctggccg aggccctcga cacgctcgcg gcctgcgact
      121 gggacgtggg cgtcgctgca aaacgtctga acgtgaccgc ctcgcagctt gtcaagctcc
      181 tgcagcgcga gccgccggct cttggccagc tcaacgacca gcgccgccta cgccagcttg
      241 tgccactcaa ataaccgtag gcttctcgct ccgcgagaag cgatcgactc gccactcggg
      301 caatctccgc gcgtctactg acctcctttg catctcgcgg agcgagatgc ctacgatgcg
      361 tacatgtcca acttcgaccg ccagaagtgg aacgccaagt accagaccga gccggcgccc
      421 cgcgagcctt cgcaggggct catcgcgctc gattgctttc tcccgcggag cggtaaggcg
      481 attgatctcg cctgtggagc ggcccggcat ggcatctggc tggcccagcg cggcctggat
      541 gtgaccatcg ccgatatctc cgccgtcggc ttgtcgctcg ccatggagcg ggcagccgag
      601 cgcggcgtct cgatcaagcc gctcgaaatc gacctggagc acgattcgtt cccccctggc
      661 ccctgggacc tgatcctcag cgtctgctac ttgcagcgat cgatgttcgc cgcctatccg
      721 acggcactgg ctcctggcgg cacgctggtc gtgattcagc cgaccaagaa gaacctcgag
      781 cgccacgcca agccccccgc cgatttcctt ttggacgagg gcgagttgcc gggcctgatc
      841 cgcagcctgg aagtcctgca ctaccaggag gcctggtcag ccgacgaccg ccacgacgcc
      901 gtccttgtcg cccgccgccc cgtggggcag gcattcttgc ctgccgggca gacgcgagtt
      961 tctgccccac aatcgggcat tgtatgatta ggtgagcgac aagccatgaa acttctccgc
     1021 gtcggcgccg ccgtcctcaa ccagacgccc ctcgactggg atggcaacct cgcgcgcatc
     1081 cgcggcgcca tcgccgccgc caaagcaggc ggcgtcagcc tcctctgcct ccccgagctg
     1141 tgcatcacgg gctacggctg cgaggacgcg tttcacgccc ccggcacgca gagcacggcc
     1201 attgatattt tgtgcgaatt gcgcggcgaa acgcgcgata tggccgtgtc attcggactg
     1261 ccgctgctgg tcaatggctc gctatacaac acggcatgct tggtcgtgaa cggccagatt
     1321 ctgggcttcg ttggcaagca gaatctcgct ggcgaaggca ttcactacga gccgaggtgg
     1381 ttcaagccgt ggccgcaggg gcaagttacc ggcgtcaaaa tccgcgacat tgcgcatgac
     1441 gttggtgacc tgctcttcga cgtaggtgga atcaacgtcg gtttcgagat ttgcgaagac
     1501 gcctgggtcg gagaccggcc gggcgtatcg ctcgccagac gtggcgtgga cgtaattttc
     1561 aatccaagtg ccagccactt tgcattcgga aagcacgaaa ttcgcaagcg gtttgtgacc
     1621 gaagggtcga gagcgttcag cgtcggttac gtctatgcca acctgttggg gaatgaatcg
     1681 gggcgtgcga tctatgacgg cgatgccatg atcgcagcgg ctggcaggct ggtcgtgacg
     1741 ggaccgcggt tttcctatgc ggacttttac gtaacgcacg ctgtcatcga cattgatgcg
     1801 attcgcatga gtcgggcgcg aaacgcgagt tttcgtccca tcattcagat tccgacacgg
     1861 gactacatcg gtggggattt cacgtttcct aagctgcgcc ccgtcaaaga gaccgccaca
     1921 caccccgcct gggaatcctc gccgcacatc aaggaggagg aattcacgcg ggccgaagcg
     1981 ctggccctgt tcgactatct ccgcaaaagc cgctcgcatg ggttcgtcgt ctccctctcg
     2041 ggcggagccg attcggctgc cgtgtcgtat ctggccgcga agctggtcga tttcgccgta
     2101 gccgagatcg gccgcgaggc gtttctcaag aagctgagct atatccccgg gctcgcccaa
     2161 gcaaccgaca acagagcctt cgtccgccgg cttttatcct gcgtctatca gccgacgcgg
     2221 aacagcggcg aggtgacccg caatgcggca gccgcggtgg ccaaggcgct tggcgcggag
     2281 tatctcgaat tcaacgtcga tgagctggtg cagggctata cttcggtcgt ctcgcaggcg
     2341 ctcggccgca agttggattg gaaaaccgac gacctggccc tgcagaatat ccaggcccgc
     2401 gcccgcgcgc cgagcgtctg gatgcttgcc aacatcaaga gggccctgtt gctggcgacg
     2461 agcaaccgct ccgaagcggc cgtcggctac gtcacgatgg acggcgacag cagcggcggc
     2521 ctctcgccga ttgccggcat
//
LOCUS       DASUGB010000751         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2028785, whole genome shotgun sequence.
ACCESSION   DASUGB010000751 DASUGB010000000
VERSION     DASUGB010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028785"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..564
                     /locus_tag="VFV87_14490"
     CDS             154..564
                     /locus_tag="VFV87_14490"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5105024.1"
                     /translation="MTLSLPSAPYLAVKVAMMPRDTNHMGTIFGGVLLSYIDQAGAIG
                     AYHEVRRVGWPARPLVTVAMNAVEFHQPVFVGDVVSFWTRVMRVGTTSITTHVSVETE
                     RDGREVRLTEAEVTYVAIDPLASGRTPQPIRGKV"
     gene            698..>2844
                     /locus_tag="VFV87_14495"
     CDS             698..>2844
                     /locus_tag="VFV87_14495"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5105025.1"
                     /translation="MANEFAIAEPPVDFSRQIRPILANNCFKCHGPDAAERQSGLRLD
                     VRDLALKAADSGELAIVPGKSAESELIQRISSADADTRMPPADEKKTLTGDEKALLAR
                     WIDEGAKYETHWAFTPPQRPSLPEVKMMGWARGELDGFILARLERAGLTRAGEANRTT
                     LIRRLTLDLTGFPPTVGEVEAFLADQRPDACERLVDRLMASPHFGERLAVDWLDAARF
                     ADTHGYHIDAGRDMTRWREYVIDAFNHNMPFDRFTVEQLSGDLLPATGDERLDLRQKL
                     ASGFNRNHMINFEGGAIPQEYLNAYIVDRVNTTGTVFLGLTVACTQCHDHKFDPLTQR
                     DFYQLYAFFNHVPEEGLDGRKGNAMPFIRVPTREQEREQAELTVKIKDAEAALAAAEP
                     EIAAAQSKWEETALAKRDQQWKTLALDAAVSKGGATLTRQDDNSVLASGENPATDTYE
                     LTASGLEFVTGIRLEALPHETFTAKGPGRSSNGNIVLTDVRLMLQPMGGQPQPVKLKS
                     ASADFSQNDFPVANAIDESPDSGWAIHPEVGKAHAAVFELERRLELPAGAKLQVILKF
                     ESKFGQHQIGRFRLSATYATNPHAAGSLPEGVVTALGAAAEQRTAEQKTEIQKYFREQ
                     VSAQSRALLADLTALKTRLEAVDQQTPTSMVMAESEKPRDTFILVRGAYDKPGEKVTP
                     EVPAFLPPLAKEAPRNRLGLARWLVSDEQPLTAR"
BASE COUNT          546 a          846 c          971 g          481 t
ORIGIN      
        1 tgtgctgatc tgcccggtcg atggggattt gatggcgagc ttagcaggcc aattggtgga
       61 accaggggaa ttctggctgc catcgcgcag ctctttaggg agcgagcgag cgcgagcgct
      121 cgtgaaattt ttcccaagca aacggggagc agcatgacac tttctttgcc gtccgcgccc
      181 tacctggccg tgaaggtcgc catgatgccg cgcgacacga atcatatggg gacaattttc
      241 gggggcgtgc tgctgagcta tatcgaccag gcgggggcga ttggcgcgta tcacgaggtg
      301 cgccgtgtcg gctggccggc gcggccgctc gtgacggtag cgatgaacgc agtcgaattc
      361 catcagccgg tgttcgtcgg cgacgtggtc agcttctgga cgcgagtgat gcgggtgggg
      421 accacatcga tcacgacgca cgtttcggtc gaaacggaac gggacgggcg ggaggttcgc
      481 ctgaccgaag cggaagtaac gtatgtcgcg attgatccgc tggcatccgg tcgaacgccg
      541 cagccgattc ggggcaaggt gtaggtggat cggttatagt gatggattac cggcagaggt
      601 gcggatgctg cgtgggaaag gtgcgaaagg gggatcccgt tcgatgactt tgttcaggcg
      661 tgcttgtgcg ggatggattg cgtgccttgc aactgtcttg gccaatgagt ttgccatagc
      721 cgaaccgccg gtcgatttca gccggcagat tcggccgatc ctggcgaaca actgcttcaa
      781 gtgccacggc ccggacgcag ccgagcggca gtcgggcctg cgactcgacg tgcgagactt
      841 ggcgctaaaa gcggccgatt cgggcgaatt agcgatcgtt cccggcaagt ccgccgaaag
      901 cgagctgatc caacggatct cgtcggccga cgcggacact cgcatgcctc cggcggacga
      961 gaagaagacg cttacgggtg acgaaaaggc tctgctcgcc cgctggatcg acgaaggggc
     1021 gaagtacgaa acacactggg cgtttacgcc gccccagcgg ccatcgctgc cggaagtaaa
     1081 gatgatggga tgggcacggg gcgaactcga tggcttcatt ctggcacggc tggagcgcgc
     1141 gggactgacg cgtgcgggtg aagcgaatcg cacgacgctg attcgcaggc tgacgctaga
     1201 cctgaccggc tttccgccga cggtgggcga agtggaggcg tttctggccg accagcggcc
     1261 ggatgcctgc gagcggctgg tcgaccgctt gatggcttcg ccgcactttg gcgagcggct
     1321 ggccgtcgat tggctcgacg ccgcccgctt tgccgatacg cacggctacc acatcgatgc
     1381 cggccgcgac atgacgcggt ggcgggagta cgtcatcgat gcgttcaatc acaacatgcc
     1441 gttcgatcgg ttcaccgtcg agcagctctc gggcgatctg ctccctgcga cgggcgacga
     1501 gcggctggac ctgcggcaga agctggccag cggcttcaac cgcaatcaca tgatcaactt
     1561 cgagggcggc gcgattccgc aggagtatct caacgcctat atcgtcgatc gggtgaacac
     1621 gaccggaacg gtcttcctcg gcctgacggt ggcctgcacg caatgccacg accacaagtt
     1681 cgacccgctt acgcagcgcg atttctacca gctctatgct tttttcaatc acgttcccga
     1741 agaagggctg gatggtcgca agggcaatgc gatgcccttc atccgcgtgc cgacgcgcga
     1801 gcaggagcgg gagcaagcgg agctgaccgt caaaatcaaa gacgccgaag ctgcccttgc
     1861 cgccgccgag ccggaaatcg ccgccgccca atccaaatgg gaagaaacgg ccctggccaa
     1921 gcgcgatcag cagtggaaaa cgctggcctt ggacgcggca gtatccaagg ggggtgcgac
     1981 actgacgcgg caggacgaca attcggtgct ggcatcgggc gagaacccgg cgaccgacac
     2041 ctacgagctg acggcgagcg ggctggagtt cgtcaccggc atccggctgg aagccttgcc
     2101 gcacgagacc ttcacggcca aaggtcccgg acgcagctcc aacggcaaca tcgtgctgac
     2161 cgacgtgcgg ctgatgctgc agccgatggg cggccagccg cagccggtga aactcaaatc
     2221 ggcttcggcc gatttcagcc agaacgattt tccggtggcc aatgccatcg acgagagccc
     2281 ggacagcggc tgggcgatcc atccggaggt tggcaaggct cacgcggccg tgttcgagct
     2341 cgagcggcgg ctggaactgc ccgctggagc gaagctgcag gtcatcctta agttcgaatc
     2401 gaaattcggc cagcaccaga ttggccggtt ccggctgtcg gcgacctatg ccacgaaccc
     2461 gcacgcggct ggctcgctgc cggaaggcgt tgtgaccgcg cttggcgctg cggccgaaca
     2521 gcgcacggcc gagcagaaga cggagatcca gaagtacttc cgcgagcagg tttcggcgca
     2581 atcgcgggcc ttgctcgccg atttgacggc gctgaagacg cggctggaag cggttgacca
     2641 gcagacgccg acgagcatgg tgatggccga gtcggaaaaa ccgcgggata cgttcatcct
     2701 ggtccgcggg gcctacgaca agccgggcga gaaggtcacg cccgaggttc ccgctttcct
     2761 gccgccgctg gcgaaggaag cgccgcgcaa ccgcctcggc ctggcccggt ggctggtcag
     2821 cgacgagcag ccgctgaccg cccg
//
LOCUS       DASUGB010000752         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_361479, whole genome shotgun sequence.
ACCESSION   DASUGB010000752 DASUGB010000000
VERSION     DASUGB010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361479"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFV87_14500"
     CDS             <1..759
                     /locus_tag="VFV87_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105026.1"
                     /translation="EVAKAYYSVPPEYLTREVWVRWDARLVRVFNHRWDQIAVHVRHE
                     QGRFSTHGQHLAKEKINGLERGASYLLNKVGLIGPQTQAWATAMLSARGIEGTRVLQG
                     LLALTKKHASEALENACETALSHDCFRLRSIRQLLQRQTARQEPLPFLDEHPLIRPLD
                     DYAQVVARALHRQADRPSVGEGFKRHDWTVISTVSAHEKSPAGVSPQGSSVRSPPRPG
                     YPLPGCTSAEPGSVSPDQPTIVPPSSPDQEHSDE"
     gene            752..1609
                     /gene="istB"
                     /locus_tag="VFV87_14505"
     CDS             752..1609
                     /gene="istB"
                     /locus_tag="VFV87_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX5105027.1"
                     /translation="MNDRLQSALHELRLSGLAQSLDVRLQEAAGHQLNHTEFLELILQ
                     DELLVRQQRQIDRRVKAAGFRELKPLDDFDWSFNPSIKRSQIFDLATCRFLREAQDVL
                     LVGPPGTGKSFLAQALGYQAIKQGHLVLYRSIFDVVRDFLHDEALEGQDRILNKYLKP
                     DLLIVDDMGMKNLPKRSGEFLFEIIMRRYETRSTMMTSNRPLEDWGKLIGDVPSATAI
                     LDRFLHHATVIPITGRSYRLRNQGRGDASPTGAEDAGNSKPAIPPTGSTTAGKRKKAA
                     AEPAETCQS"
     gene            1688..1846
                     /locus_tag="VFV87_14510"
     CDS             1688..1846
                     /locus_tag="VFV87_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX5105028.1"
                     /translation="MTTELEMTEYQDSRQARQDIASYLAYYNHDRRHSSLNYLSPAAF
                     EAQLHRSI"
BASE COUNT          403 a          689 c          569 g          355 t
ORIGIN      
        1 gaggtagcca aggcgtatta ctcggtgccg ccggaatatc tgacccgcga ggtctgggtg
       61 cggtgggacg cccggctggt gcgagtcttc aatcaccgct gggaccagat cgccgtgcat
      121 gttcggcacg agcaaggtcg cttcagcacg cacggccagc acttggcgaa ggagaagatc
      181 aacggcctgg agcgcggagc gagctacctg ctgaacaagg tcggactgat cggtccgcag
      241 acgcaggctt gggccacagc aatgctctcc gcccgcggca tcgaagggac gcgggtgctg
      301 cagggcctgc tcgcgctgac gaagaagcac gccagcgaag ctctcgaaaa cgcctgcgaa
      361 acggcccttt cgcacgattg cttccggcta cgttcgattc gccagttgct ccagcgccaa
      421 acggcccggc aagagccgct gccgttcctc gacgagcatc cgctgattcg cccgctggat
      481 gattacgccc aggtggtcgc gcgggcgctc catcgccagg cggatcgccc ctccgtgggc
      541 gaaggtttta aaaggcatga ctggacagtg atctccacgg tgagcgcgca cgaaaaaagc
      601 cccgcgggcg ttagcccaca aggctcgtcg gtgagatcac cgcccaggcc tggctatccc
      661 ttgccgggtt gcacctctgc agagcccggc tccgtttcgc cagaccagcc cacgatagtt
      721 ccgccttctt cccctgacca ggagcactct gatgaatgat cgcttgcaat cggccctgca
      781 tgagctgcgg ctctcgggcc tggcccagtc gctcgatgtc cgtctgcagg aagcagcggg
      841 ccaccagctc aaccacaccg agttcttgga gttgatcctc caggacgaac tgctcgtccg
      901 ccagcagcgg cagatcgacc gccgcgtcaa agcggccggc ttccgcgagc tgaagccgct
      961 ggacgacttc gactggtcgt tcaacccgtc gatcaagcgc tcgcagatct tcgacctggc
     1021 aacctgccgg ttccttcgcg aagcgcagga cgtcctgctc gtcggcccgc cggggaccgg
     1081 caagagcttc ctcgcgcagg ctcttggtta ccaagccatt aaacaagggc acctcgtgct
     1141 gtaccgctcg atcttcgatg tggtccgcga cttcctccac gacgaggccc tggaagggca
     1201 ggaccggatc ttgaacaagt acctcaagcc ggacctcttg atcgtggacg acatggggat
     1261 gaagaatctg ccgaagcgga gcggcgagtt cctgttcgag atcatcatgc ggcggtacga
     1321 aacccgctcg acgatgatga ccagcaaccg gccgctggag gactggggca agctgatcgg
     1381 cgacgtcccc agcgccacgg cgatcctcga ccgcttcctg caccatgcca ccgtgatccc
     1441 cattaccggc agaagctatc ggctgcggaa ccagggtcgc ggcgacgctt cgcccactgg
     1501 cgcagaggat gcaggaaact caaaaccagc cattccgccc accggctcta cgaccgccgg
     1561 caaacgcaaa aaagccgccg ccgaaccagc cgagacttgc cagtcctaac gattccgacg
     1621 atcatccacg tgacccttca ccccggctgc gaattctgcc cgcgactggc tgcaattaat
     1681 ccgcccgatg acaaccgaac tcgaaatgac cgaataccaa gacagccgtc aggcccggca
     1741 agatatcgcc agctacctcg cctactacaa ccacgaccgc cggcattcct ccctgaacta
     1801 cctcagccca gccgctttcg aggctcaact ccaccgttca atttaagcaa ctcgactgtc
     1861 cgaaatttgc caagcagctc agctcgcctc accggcgtct tcggtcaatc accagtcgga
     1921 agtcagcatc ctttcggatt ggccatcgga cctatccagc cggttatcac ttcccgttgc
     1981 ctttcggctg ctggcattcg ctttttccga catccc
//
LOCUS       DASUGB010000753         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_638427, whole genome shotgun sequence.
ACCESSION   DASUGB010000753 DASUGB010000000
VERSION     DASUGB010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638427"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFV87_14515"
     CDS             <1..263
                     /locus_tag="VFV87_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5105029.1"
                     /translation="EADFDQVVVRIVWTESGPSWLVNDAPVASLAELRGALIAIARIK
                     SDAPVILDPDQIVPLGDVIDVFDLSRLVGFEKIQFATSIGGG"
     gene            273..2582
                     /locus_tag="VFV87_14520"
     CDS             273..2582
                     /locus_tag="VFV87_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105030.1"
                     /translation="MRIVQSPKSKAQSLAVVLVGAMLALPAIVWAESSDARFLDGLRQ
                     RQLFELAEEYCSYRLARSVANGPIQADLSIELIRTLALHATNVPPAERDGPWAKARSV
                     AAEFAARKPPHPRAILVRLQDALTPLAQGELARQELEAGALTDDRLEQARAALRESRK
                     LFDELSQELAREIPFRRRTAPEKGELSADQLFNLQQHVQYQLARAQRNQALLFAKGSD
                     DRLALLLAAVETLQGLMSQIAADEPLMPQVQLDLSECQRLLGRLAEAEEIAGTLDREG
                     VPPAVRLRARAELLRSAIAEEDLSAIQKLLALGREVDGEAAADLDFAWFEAFLAQARL
                     ASEGMRAAGPKPAAEVSKQFQDQAAAAADFLEESYGPYWGRRANQLLIASLPRGGRGN
                     VQLLARAADSLYLKGEFEQAIAAYDEAAAQARQSSDLAASFDLAYKAALIEQKRERHA
                     QAAQRLRILSKSFATHAQAAPAHLLAAWNAAQAARDNAAAADSYAAILREHLATWPAA
                     ESADQARIWLGKIEESQSNWADATEAYSGVPRSSPHFAAAAVGLARCWPKRLAGLSSA
                     GKPTAGVADEAIQALQRALLDPENRLPQTWTESDRAVALALAELIVGYQPASSADAVK
                     ILEAASNTSDATAEWKAAAQIPLVLALANQPVRREEAVAALGRLAKENPNSGAIQERY
                     ADLLLAAHDAASLQQALERWRVIASRSQPRTERWYKAKLSIAQAQYLLGEKHEAAVLL
                     HYLLETQPSLKGTAWETAYRELLGKCEAR"
     gene            2741..3058
                     /locus_tag="VFV87_14525"
     CDS             2741..3058
                     /locus_tag="VFV87_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105031.1"
                     /translation="MFRAILALVALCAFTTLALAAPAIAPQQDARFEGKVMAVTADSI
                     TIMTDRQMDVLTFAVNAQTKVTYNGEQAQLIDIQMGDQATVTGKVMEQDRIATAIVAN
                     RRM"
     gene            3096..>3427
                     /locus_tag="VFV87_14530"
     CDS             3096..>3427
                     /locus_tag="VFV87_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105032.1"
                     /translation="MNGRLIMGVALIGAAGIFSLWPRASVAVPAEAFEGIVIAVAKDA
                     LTAMDALVPRKVTFEVKQNVRITRNGELARMDEIQPGDAVRIFVRPLGDKLLALSIEA
                     RSPRPTAPL"
BASE COUNT          671 a         1065 c         1104 g          587 t
ORIGIN      
        1 cggaggccga cttcgaccag gtcgtggtcc gcatcgtctg gacagaatcc ggcccctctt
       61 ggctggtcaa cgacgcgccg gtcgcttctc tggccgagct gcgcggggcg ctcatcgcaa
      121 tcgcacggat caaaagtgat gcgcccgtga tactcgatcc cgaccaaatc gtgccgctgg
      181 gggacgtcat cgacgttttt gatttgtccc ggctggtggg atttgagaaa atacagttcg
      241 cgacgtcgat cgggggcggg tgagtcaagg caatgcggat cgtccaaagt ccaaagtcca
      301 aggcccaaag tctggcagtc gtcttggtcg gggcgatgct cgctctgccg gcaattgtct
      361 gggcggagtc ctcggatgcg cggttcctgg acggcctgcg gcagcggcag ctctttgagc
      421 tagcggaaga gtactgctcc tatcgtctgg cccgcagcgt tgccaatgga ccgattcaag
      481 ccgatctgtc gatcgaactg attcgcacgc tcgccctgca cgcgaccaac gtcccaccgg
      541 ctgagcgtga tggaccctgg gccaaggccc gcagcgttgc agcagagttc gccgcccgaa
      601 aaccgccgca tccgcgggcc attctcgtcc gcttgcagga cgcgctcacg ccgctcgcac
      661 agggcgaact tgccaggcaa gagttggagg cgggagcgct taccgacgac cgactggaac
      721 aggcgcgagc cgccctgcgc gaatccagaa agctgttcga tgaactgagc caagagctgg
      781 cgcgagaaat tcccttccgc cgccgcaccg cgccagagaa gggcgagctg tctgccgacc
      841 agctcttcaa ccttcagcag cacgtccagt accaattggc tcgcgcccag cgcaatcagg
      901 ctttgctctt tgccaaggga agcgacgacc gcctggcctt gctgctcgcc gccgtcgaga
      961 cgctccaggg actaatgagt caaatcgccg ccgatgagcc gctcatgccg caggtccagc
     1021 tcgatctgtc cgaatgtcag cggctgctgg gccggttggc ggaagccgaa gagattgcgg
     1081 gaacgctcga ccgggaaggc gtccctcctg ccgttcggtt gcgggcgcgt gcagagctgc
     1141 ttcgctcggc aatcgccgag gaagacctat ccgccattca gaagctgctg gcgttgggac
     1201 gcgaagtgga cggggaagcg gcggcggatt tagatttcgc ctggttcgag gccttcctcg
     1261 cccaagcgcg cctggccagc gaaggaatga gggccgcggg gccaaaaccc gcagcggaag
     1321 tctcgaagca gttccaggac caggccgcgg ctgcggctga tttcctcgaa gagtcgtacg
     1381 gaccctattg gggccgacgg gccaaccagt tgctcatcgc ctccctcccg cggggtggcc
     1441 gaggcaacgt gcaactcctt gcccgcgcag ccgacagcct gtatctcaag ggcgaattcg
     1501 agcaggctat cgcggcctat gacgaggccg cggcccaagc ccgccaaagc agcgacctgg
     1561 ccgcaagttt cgacctggcc tacaaggcag cgctgatcga gcagaagcgc gagcggcacg
     1621 ctcaagcagc gcagcgcctg cggattctct ccaagagctt tgcaacccac gctcaggccg
     1681 cgccggcgca tttgctggca gcgtggaacg ccgcgcaggc cgcccgtgat aatgccgcgg
     1741 ccgccgattc gtatgccgca atcttgcgcg agcacctggc cacctggccg gcggcggagt
     1801 ccgcggacca ggctcgcatt tggttgggca agatcgaaga gtcgcaatcg aactgggcag
     1861 acgccaccga agcgtattcc ggcgtgccgc gcagtagtcc gcactttgct gcggccgcgg
     1921 tcggcctggc acgctgctgg ccgaagcggc tggcggggtt gtctagcgcc ggaaagccca
     1981 cggccggtgt cgccgacgag gcgattcaag ccctgcagcg ggcgctgctc gatcccgaga
     2041 accggctgcc gcaaacgtgg accgaatccg accgcgccgt cgcactggcg cttgccgagc
     2101 tgattgtcgg ctatcagccg gcaagttccg ccgatgccgt aaagatttta gaagcggcct
     2161 cgaatacttc ggacgcgaca gccgagtgga aggccgctgc ccaaattcca ctcgtcctgg
     2221 ctttagccaa tcagccggtc cgccgcgaag aagcggtggc agcactgggg cggctggcga
     2281 aggaaaaccc gaacagcggc gcgattcagg aaaggtacgc ggatctcttg ctcgcagccc
     2341 acgatgccgc gtcgctccag caggcactcg aacgctggcg agtgattgcc agccgcagcc
     2401 agccccggac cgagcgctgg tataaggcga agctttcgat cgcccaggcc cagtatcttc
     2461 tgggcgaaaa gcacgaggcc gccgtactgc tccactatct gctcgaaacg caaccgagcc
     2521 tgaagggaac ggcgtgggaa accgcctatc gcgaactgct cggaaagtgt gaagcacgtt
     2581 gacgcatcgc gcgctgtgac atgctggcac accgctgcac gcgctctgct gagaattctg
     2641 cggctttcaa ggaacaaaaa ctttggcacg cgacctgcac ggttgtggta ttggtctcta
     2701 atcggagacg gaacccacga cccgaaagga gggtgcgaca atgttccgag caattttggc
     2761 gctggttgct ctttgcgctt tcacaactct tgcgttggct gcccccgcga tcgcgcccca
     2821 gcaagacgca cgcttcgagg gcaaggtgat ggcagtaaca gccgactcga tcacgatcat
     2881 gaccgatcgg cagatggatg ttttgacgtt cgctgtcaac gcccagacaa aggttaccta
     2941 caacggcgag caggcccagc tcatcgatat tcagatgggc gaccaggcga cggtcaccgg
     3001 aaaagtgatg gagcaagacc ggattgcaac cgccattgtg gccaatcggc gtatgtagag
     3061 tcttgagaaa tggaaaaaag cgtggaggca atttgatgaa cggtcgccta atcatggggg
     3121 tcgcgctgat tggcgcggcc gggattttct cattgtggcc gcgcgcctcg gtggcggtac
     3181 cggcagaagc ttttgaaggg atcgtcattg ccgtggccaa agacgcgcta acggccatgg
     3241 acgcactcgt cccgaggaag gtcacttttg aggtcaagca gaacgtcagg atcacccgga
     3301 acggggaact ggcgaggatg gacgagatcc agccgggaga cgcggtccgc atcttcgtcc
     3361 gcccgctggg cgacaagttg ttggcactga gtatcgaggc gcgatcacca cggccgaccg
     3421 cgccgct
//
LOCUS       DASUGB010000754         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_638592, whole genome shotgun sequence.
ACCESSION   DASUGB010000754 DASUGB010000000
VERSION     DASUGB010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638592"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1075
                     /locus_tag="VFV87_14535"
     CDS             236..1075
                     /locus_tag="VFV87_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEX5105033.1"
                     /translation="MALFTRDEILARLRAKVAAGQPIVGGGAGTGISAKMSEAGGIDL
                     LVIYNSGRFRMAGRGSLSGMMPYGDANAIVLEMAREVIPVVERTPVLAGVCGTDPFRL
                     MKLFLKEIDAAGFSGVQNFPTVGLIDGTFRQGLEETGMGYGLEVDLIRTAHEMGLLTT
                     PYCFNDDEAAAMAKAGADILIPHMGLTTKGTIGASTALSLEDSAKRVQSMHDAAKRVN
                     KDILVLCHGGPIAEPADAQYVLDHTSGIVGFYGASSMERLPVEPAIRDRVREFSALRF
                     KNA"
     gene            complement(1062..1310)
                     /locus_tag="VFV87_14540"
     CDS             complement(1062..1310)
                     /locus_tag="VFV87_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HEX5105034.1"
                     /translation="MSTIRVHFDGKVFVPDEPVNVPEGTPAQVIVIPQSEASKSATGR
                     KPLAELAAILDAMPSESSLPPDFSTNLDHYLYGMPKRS"
     gene            1426..1947
                     /locus_tag="VFV87_14545"
     CDS             1426..1947
                     /locus_tag="VFV87_14545"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX5105035.1"
                     /translation="MTLNAAHIVVTGLLSADDAIRAMKVLGKWRPFRTGLLFVAFLLV
                     IAAIATTQKPDIRIHVLVVVGAIGSLVAIGWASSVRDKFTKQFCANPEIANPIVWTFS
                     GEGLLIQTANTSSLHAWNSFEKATITPTVVILAHHGGLSFNFIPRRLFASEADYSATC
                     QLISAKLPVRQLG"
     gene            2027..>3000
                     /locus_tag="VFV87_14550"
     CDS             2027..>3000
                     /locus_tag="VFV87_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5105036.1"
                     /translation="MQAVIDVSVAAERPNILWISLEDISPNLGCYGDEYAVTPNIDRL
                     AAEGCRYTQAFSHAGVCAPTRSGIITGMYPTSIGTHYMRCKGVPPSHVKCFTEYLRSR
                     GYYCTNDNKTDYNFDSPLTAWDDNRKGAHWRNRPSKETPFFCVINLTTTHESQIRLPE
                     AQLENRRKTLSADELHDPAKAPLPPYYPDTPVVRRDVANYYDNLTWTDREVGRILKQL
                     ADDGLADSTIVFFWGDHGWGMPRGKRWIYDSGIRVPLVVRWPGQLQPGSASDELVAFV
                     DLAPTMLSLAGAPIPKHLQGQAFLGEQKAPPREYIYAARDRMDETLDI"
BASE COUNT          574 a          909 c          919 g          598 t
ORIGIN      
        1 gcggtcccaa ttactggcac accacgcagg acgtccccga gcattgttcg gccctctcgc
       61 tggccaaagt cggctgggtc gtcctcgaat ggacgaagcg ggcgaagtag catggtatgt
      121 ccttagccgt agggtgggac cagcgagcgg agcgagcgca ggcccaccaa caccatcaac
      181 attgacccga tttggtgggc cagcgcccgc ggcgggcttg tcccacccta cgatcatggc
      241 tctctttact cgcgacgaaa tcctcgcccg cctccgcgcc aaagtcgccg ccggccagcc
      301 gatcgtcggc ggaggcgcgg ggacgggcat cagcgcgaag atgtcggaag cggggggcat
      361 cgaccttttg gtgatctaca actccggccg cttccggatg gccggccggg gcagcctctc
      421 cggcatgatg ccctacggcg acgccaacgc aatcgtcctg gaaatggccc gcgaggtgat
      481 tccggttgtg gaaaggacgc ccgttctcgc cggcgtttgc ggtaccgatc cctttcgcct
      541 gatgaagctg tttctcaagg aaattgacgc cgccggcttc agcggcgtgc agaattttcc
      601 gacggtcggc ctgatcgacg gcacgtttcg ccaggggctg gaagaaacgg ggatgggcta
      661 cggcctggag gtcgatctga tccgcacggc ccacgaaatg ggcctgctga cgacccccta
      721 ctgctttaac gacgacgaag ctgcggccat ggccaaggcc ggcgcggaca tcctcatccc
      781 gcacatgggg ctgacgacga aaggcacaat cggggcatcc acagcgctat cacttgagga
      841 ctcagcgaag cgcgtgcagt cgatgcacga cgccgccaag cgcgtgaaca aggacatcct
      901 cgtcctctgc cacggcggcc ccatcgccga acccgccgat gcccagtacg tcctcgatca
      961 caccagcggc atcgtcggct tctacggcgc ttcgagcatg gagcgccttc ctgtcgagcc
     1021 ggccattcgc gatcgcgtgc gggagttttc ggcactgcga ttcaagaacg cttaggcatg
     1081 ccgtagaggt agtggtcgag attcgtggag aagtcggggg gcagagacga ttcggagggc
     1141 atggcgtcca gaattgccgc cagctccgcc agcggcttgc ggccggtcgc ggacttgctc
     1201 gcctcgcttt gcggaataac gatcacttgc gccggcgtgc cttcgggtac gttgaccggc
     1261 tcgtcgggta cgaatacctt accgtcaaaa tgaacgcgga tggtggacat ggctattctc
     1321 ggtcctgtca ttgtaccata agcgtcgtcg gcttctacga cgattctggg gcggtgcgcc
     1381 ggcccatcga gccggtgatt cgagtgcgcg ggatttagaa ttacgatgac cctcaatgca
     1441 gcacatattg ttgtcacggg ccttctttcg gcagacgacg caatacgggc gatgaaggtc
     1501 ttgggcaagt ggcgtccatt tcgaaccggc ctactctttg tcgctttctt gcttgtgatt
     1561 gcggcgatcg ctacaactca gaaaccagac attcggattc atgtgctggt cgttgtgggt
     1621 gcgatcggat cactggttgc aatcggttgg gcttcgagtg ttcgtgacaa attcaccaag
     1681 cagttttgcg caaatccaga gatcgcgaat cctatcgttt ggaccttttc gggcgaaggg
     1741 ctactgatcc aaacagcgaa cacgagttcg ctacacgcgt ggaattcgtt tgagaaggcg
     1801 acgattacgc ctacggtcgt cattctcgca catcacggcg gcttgtcgtt caacttcatt
     1861 ccccgccgac tctttgcttc agaagccgat tactcagcga cttgccaatt gatctctgcg
     1921 aagttgccgg ttcgtcaact tggttaaagc tgactcatct cacgacattg aaaacacgag
     1981 gtacatgatg gcgaggcttg ccttttgcat tgtgtcgttg tttgtaatgc aagctgttat
     2041 agatgtgagc gtcgccgccg agcggccgaa catcctctgg atcagcctgg aggatatcag
     2101 cccgaatttg ggctgttatg gcgacgagta cgccgtgacg ccgaatatcg accggctggc
     2161 ggcagagggc tgccggtaca cgcaggcctt ttcgcatgcc ggcgtttgcg ctccgacacg
     2221 aagcgggatc atcacgggga tgtatcccac gtcgatcggc acgcactata tgcgctgcaa
     2281 gggcgtgccg ccgagccatg tgaagtgctt tacggagtac ctgcgctccc gcggctatta
     2341 ctgcaccaac gacaacaaga ccgactacaa cttcgattcg ccgctgaccg cgtgggacga
     2401 caaccgcaag ggggcgcact ggcggaaccg gccgagcaaa gagacgcctt ttttttgcgt
     2461 catcaatctg accacgaccc acgagagcca gattcgtctg ccggaggcgc agctcgagaa
     2521 tcgtcgtaag accctctcgg cggatgagct gcatgatccg gcgaaggccc cgctgccgcc
     2581 gtattatccc gatacgccgg ttgtccgccg cgatgtggcc aattactacg acaacctcac
     2641 ctggaccgac cgcgaagtcg gccggattct caagcagctc gcagacgacg gcctggcgga
     2701 tagcacgatc gtcttcttct ggggcgatca cggctgggga atgccccgcg gcaagcggtg
     2761 gatttacgac tcgggcattc gcgtgccgct cgtcgtccgc tggccgggcc aattgcagcc
     2821 ggggagcgcg agcgatgaac tggtcgcgtt cgtcgatctg gcgccgacga tgctctcgct
     2881 ggccggcgcc cccatcccca agcacctgca ggggcaggcc ttcctgggcg agcagaaagc
     2941 gccgccgcgc gagtacatct atgccgcccg cgaccggatg gacgagacgc tcgacatcat
//
LOCUS       DASUGB010000755         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1195091, whole genome shotgun sequence.
ACCESSION   DASUGB010000755 DASUGB010000000
VERSION     DASUGB010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195091"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..303
                     /locus_tag="VFV87_14555"
     CDS             40..303
                     /locus_tag="VFV87_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105037.1"
                     /translation="MRILIAVPLVLLAACQVSKDENSTTVSFNEDVAENAAADAGNFA
                     ENVGGMIANDVDQTADKVKNEVGDVDVDVNVSHDNDAHDGNHQ"
     gene            347..423
                     /locus_tag="VFV87_14560"
     tRNA            347..423
                     /locus_tag="VFV87_14560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Pro,seq:tgg)
     gene            complement(437..823)
                     /locus_tag="VFV87_14565"
     CDS             complement(437..823)
                     /locus_tag="VFV87_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105038.1"
                     /translation="MKTLTPLILLAAATAACTASPPPEQAVAAGGVSCLPGDQVIARH
                     LAAPNTIDFEMVGGKTYRNQLASKCVSLERLGSTAIVGVTSAADPARVCAGDRVKIFD
                     PVEVKATGLRGEPYCQLGQFTAMPTP"
     gene            complement(848..2020)
                     /locus_tag="VFV87_14570"
     CDS             complement(848..2020)
                     /locus_tag="VFV87_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX5105039.1"
                     /translation="MRESEVLDRRTAIASGFAAALASRAQPAISASRSPDSLAGLAAR
                     SGRRFGSAVAWGRPRADRGSFANPAYAAILERECTLLVPENELKWQWTRPGPTRFDFR
                     QADAIADYAKAHDFGLRGHTLLWLPQKWYPKWLVGHDFGPQPAAAAEAMLKEHVQTVC
                     RRFGSRIYSYDVVNEAVQPETGKVRDTVITKALGHEATLDFMFHTARAEAPQAQLVYN
                     DYMSWERNSDDETHIRGVLKLLEGFRKRGTPVDALGIQSHIRLLKPLSVAELVRESEG
                     PWRRFVDEVVAMGYKLVITEFDVNDRMAPANIMQRDRMVADYAKAYLDLMLSYPQLRD
                     ILAWGMVDRYSWLNNFDPRRDKLLKRGTPYDANFRPKPLRQALAAAFASATVHASA"
BASE COUNT          405 a          650 c          675 g          418 t
ORIGIN      
        1 cgggccaagc ggcgttaaga cagcaaaagg gaggacatca tgagaattct tattgccgtt
       61 ccgctggtgc tgcttgctgc ttgccaggta agcaaggacg aaaattccac gaccgtttcg
      121 ttcaatgaag acgtggctga aaacgccgcc gctgacgccg gcaacttcgc cgagaacgtg
      181 ggagggatga tcgccaacga cgtcgatcag accgccgaca aggtcaagaa cgaggtcggc
      241 gacgtggatg tcgacgtgaa tgtcagccac gacaacgacg cgcacgacgg aaatcaccaa
      301 tagcgcggtt gtccctgcgc tagccttcgt ctagcaggcg cgccgtcggg gagtagcgca
      361 gcctggtagc gcatctgctt tgggagcaga gggtcgcagg ttcgaatcct gtctccccga
      421 ccactcgtca gctcgttcag ggtgtcggca tcgccgtgaa ctggccgagc tggcagtagg
      481 gttcgccccg caacccggtg gccttcactt cgacaggatc gaaaatcttg acgcgatcgc
      541 cggcgcagac cctcgcggga tcggctgcgc tggtgacccc cacgatagct gtcgagccaa
      601 gccgctcgag actgacgcac ttgctcgcaa gctggtttcg gtaggtcttg ccgccaacca
      661 tctcgaaatc gatggtgttc ggagcggcaa gatggcgggc gatgacctgg tctcccggca
      721 ggcagctcac gccaccggca gcgacggcct gctcaggtgg gggagaagcc gtgcatgcgg
      781 ccgttgccgc cgcgagaagg atcaagggag tgagtgtctt catgactttc tccttcgccg
      841 ccgattctca cgcgcttgca tgaaccgtcg ctgaggcaaa tgctgccgcg agggcctgcc
      901 gcaacggctt agggcggaaa ttcgcatcgt aaggggtgcc gcgcttgagc agtttgtccc
      961 tgcgcgggtc gaaattgttc aaccagctgt agcgatcgac catcccccag gcgaggatat
     1021 cccgaagctg cgggtagctc agcataagat cgagataggc cttggcgtag tcggcaacca
     1081 ttcgatcgcg ctgcatgatg tttgccgggg ccatcctatc gttgacgtcg aattcggtga
     1141 tgaccagctt gtatcccata gcgacaactt catcgacgaa ccgacgccag gggccttcgg
     1201 actcacgaac aagctccgcg acgctgagcg gcttgagaag gcggatgtgc gactggattc
     1261 cgagcgcgtc gaccggcgtt ccccgcttgc ggaagccttc gagcagcttg agcacgccgc
     1321 gaatgtgcgt ctcgtcgtcg ctgttccgct cccagctcat gtaatcgttg taaacgagct
     1381 gggcctgcgg agcctcggct cgcgccgtgt ggaacataaa gtcgagagtt gcctcgtgcc
     1441 ccaatgcctt ggtgatcacc gtgtcgcgga ccttgcccgt ttcaggctgc acggcttcat
     1501 tgacgacgtc atacgaataa atgcgtgagc cgaagcgccg gcatacagtc tggacgtgct
     1561 ccttgagcat cgcttccgcg gctgccgcgg gttgcggccc gaaatcgtgc ccgacgagcc
     1621 atttcggata ccatttctga ggaagccaga gcagtgtgtg ccctcgcagg ccgaaatcat
     1681 gcgccttcgc ataatcggcg atggcatccg cctgacggaa gtcgaacctc gtcggtccgg
     1741 gtcgggtcca ctgccacttc agttcgttct caggaacgag cagcgtgcat tcacgctcga
     1801 ggattgcggc ataggccgga ttggcgaagc ttccacgatc tgcacgcgga cgaccccagg
     1861 ccaccgccga accgaagcgg cgtccactgc gcgcggccag acccgcgagt gaatctggcg
     1921 atcgcgatgc cgatatggcg ggctgagcgc gcgatgcgag cgcagcggca aagccggacg
     1981 cgatggcggt tcttctgtcg agcacttcgc tctcccgcac ttgatttcct gtgatagcgc
     2041 taacaggctc acgttcgtgc gcaagcttct gttgcttctg ctcgtcacct ttgcgtctcc
     2101 ggcagcggct gaggacggct acgagctctg gctgcggtac cgcctggt
//
LOCUS       DASUGB010000756         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_778815, whole genome shotgun sequence.
ACCESSION   DASUGB010000756 DASUGB010000000
VERSION     DASUGB010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778815"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV87_14575"
     CDS             <1..204
                     /locus_tag="VFV87_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HEX5105040.1"
                     /translation="LINVPAGTQHWFDLCEDKTIRCIRLFQDQSGWTPHYVENSVHEK
                     YLPVCLGPAYVKPAAEFQPVVKV"
     gene            complement(548..1954)
                     /locus_tag="VFV87_14580"
     CDS             complement(548..1954)
                     /locus_tag="VFV87_14580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105041.1"
                     /translation="MTQNPYEPSKIPATPKEPASPGDDLSQTGRNIILAAAFLGWMFS
                     GFQMALMTLSARAATTEFIRTGQVSASQPLDLQRLLAPLCLPTDSPPAISGEKEIMKQ
                     IGGPWFSWYQAAFLLGAAGGGLVFGWLGDHLGRVRAMGASIFCFSIFSGLGYFVASPE
                     QLLLLRVLSGMGVGGMWPTGVSLASEAWSDASRPMISGLLGTSANVGLVALNVLGYFF
                     HVVPDSWRWTQLVCGAPVFLAILVWAMVPESRRWLATRNQPHLAKQGASMATVFRPPL
                     LRLTLIGIAVGTIPLLGGWGATQWFIPWADKVGGAADPSAKALAGIMRSGGACIGGLI
                     GGWLASLAGRRLTYFLVSLASFGLGEFIYRMLTPSDAAFWPFVFVLGLVSTIYFGWLP
                     LYLPELFPTHARATGAGVSFNFGRILTAVGVLGAGAITAAFNDDYARAGSITTLIYAV
                     GMIVILFAPDTTKKRLED"
     gene            complement(2042..2794)
                     /locus_tag="VFV87_14585"
     CDS             complement(2042..2794)
                     /locus_tag="VFV87_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5105042.1"
                     /translation="MNLTDLKWSDIAPLSRDVPVVIPIAALEQHGRHMPLFTDSMLLG
                     EVIRRASEPLKDRVLFTPLMWLGNSEHHLDFPGTMSAAPRVYLDLLRDMAENFLFHGF
                     KRIVFVNGHGGNIVPSQQAVFELRQKYRSQADLLLLTTTYWTLGGKPWEADPSMVQRR
                     MGHACEFETSMMLRLNPHLVSDVTEDVDWGNPFEPANRGWITKDRTGPGHIGYPRHAT
                     ASKGEILFRTFAADVVALLERVIKWDGKSWEG"
BASE COUNT          597 a          902 c          944 g          460 t
ORIGIN      
        1 ctgatcaatg tgcccgccgg cacgcagcat tggttcgact tgtgcgagga caagacgatc
       61 cgctgcatcc ggctgttcca ggaccaatcg ggctggacgc cgcattacgt ggaaaacagc
      121 gtccacgaaa agtacctgcc cgtctgcctc ggccccgcct acgtcaagcc cgccgccgag
      181 tttcagccgg tggtgaaggt gtagggtggg ctgtgcccac caagccttgc ggttgtgaca
      241 tggttttgct gcgtttcgag tgtgaaggcc gcggatggca acactttgta agcgagtctt
      301 gggggccctg ccgtcgattc gtggaggttc tcaacgacca aagagccaac cgacagatag
      361 acgtttacga aaacggctac gcgctcaaat acgaccgaca gcatgctcgt gacgaatacg
      421 gaatgctgac cgcgcacctg ttcagtcgca aacagaaatg gcagaagtcc ttcgcacgtg
      481 tacgaacgat ttcgacttcc gaattcgaca aggcgtggac gaagtacgca cgcgttgtcc
      541 cggttagcta atcctccagc cgtttcttcg tcgtatccgg cgcgaagagg atcacgatca
      601 tccccacggc atagatcagc gtcgtgatgc tgccggcgcg ggcgtagtca tcgttgaagg
      661 cggcggtgat cgcgccggcc ccgagcacgc cgaccgcggt cagaatgcgg ccgaagttga
      721 acgaaacgcc ggcgccggtc gcccgggcat gcgtcggaaa gagttccggc agatagagcg
      781 gcagccaccc gaagtagatc gtcgaaacca ggccgagcac gaaaacgaac ggccaaaacg
      841 cggcatcact cggcgtgagc attcgataga tgaactctcc cagcccgaac gacgccaggc
      901 tgaccaggaa gtacgtcagc cgtcggccgg cgaggctagc gagccacccg ccgatgagcc
      961 cgccgatgca cgcgcccccc gagcgcatga tgccggccag ggccttggca ctgggatcgg
     1021 ccgcacctcc gaccttatcg gcccacggaa tgaaccactg cgtcgccccc cagccgccga
     1081 gaagcggaat tgtgccgacg gcgatgccga tcagcgtcag ccgcaacaat ggcgggcgaa
     1141 agacggtcgc catcgaggcg ccctgtttag ccaggtgggg ctgattacgc gtggcgagcc
     1201 agcggcgcga ttcggggacc attgcccaga cgagaatcgc gaggaaaacg ggcgcgccgc
     1261 aaacgagctg cgtccagcgc caagagtcgg ggacgacatg aaagaagtag ccgaggacat
     1321 tcagcgccac gaggcccacg ttggccgaag tgccgaggag gccggaaatc atcggccggg
     1381 aggcgtccga ccaggcttcc gaggcgagcg agacgcctgt cggccacatg cctccgacgc
     1441 ccatgccgga cagcacgcgc agcagcagga gctgttcggg cgaggcgacg aaatagccca
     1501 ggccggaaaa aatcgagaag cagaagatgc tcgctcccat cgcccgcacg cggcccaggt
     1561 gatcgcccag ccagccgaac accagcccgc cccccgctgc acccagcaga aacgcggcct
     1621 ggtaccaaga gaaccacggt ccgccgatct gtttcatgat ctccttctcg ccgctgatgg
     1681 ccgggggcga gtcggtcggc aggcacagcg gggcgagcag gcgctgaagg tcgagcggct
     1741 ggctggcgga gacctggcca gtgcggatga attccgtcgt cgccgcacga gccgagaggg
     1801 tcatcagcgc catctgaaag ccactgaaca tccagccgag gaaggcggcg gcgaggatga
     1861 tgttgcggcc tgtttgcgaa aggtcatcgc caggcgacgc cggttccttc ggagttgcgg
     1921 ggatcttcga gggctcgtag gggttctgag tcatgcgact ttctggcagg cgagatgcct
     1981 accccacgtt ggcagacaag atgcctaccc cacgttggca ggcgagacgc ctaccccacg
     2041 ttcagccctc ccacgatttg ccgtcccatt tgatgacgcg ctccaggagc gcgacgacat
     2101 ctgcggcaaa cgtgcggaag agaatctcgc ccttcgacgc cgtggcgtgc cgcggatagc
     2161 cgatgtggcc gggccccgtg cggtctttgg tgatccagcc gcgattggcg ggctcgaagg
     2221 ggttgcccca gtcaacatct tccgtcacgt cgctgaccag atgcggattc agacggagca
     2281 tcatcgatgt ttcaaactcg caggcgtgcc ccatccgccg ctggaccatc gacgggtccg
     2341 cttcccacgg cttgccgccc agcgtccagt aggttgtcgt cagcaaaagc aaatcggcct
     2401 ggctgcgata cttctgccgc aattcgaaaa ccgcctgctg cgacgggacg atgttcccgc
     2461 catggccgtt gacgaagaca atccgcttga agccgtgaaa caggaagttc tcggccatgt
     2521 cgcgcaagag gtcgagatag acgcgcggcg cggccgacat cgtgccggga aaatcgaggt
     2581 ggtgctcgga atttcccagc cacatgagcg gggtgaagag cacgcgatcc ttcaacggct
     2641 cgctggcccg gcggatcacc tcgcccagca gcatgctgtc ggtaaacagc ggcatgtgcc
     2701 gcccgtgttg ctccagcgcg gcgatgggaa tgacgaccgg cacatcgcgg ctgagcgggg
     2761 cgatgtcgga ccacttgaga tcggtgaggt tcatcggcgg ctaaggcggg acggaaatgg
     2821 agtcggcccg tagcttaggc cattgggtgg ccggtcgcaa tcaacttgcc ggcgatgaat
     2881 ggcagcgaag tgccgcggcc agg
//
LOCUS       DASUGB010000757         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_223774, whole genome shotgun sequence.
ACCESSION   DASUGB010000757 DASUGB010000000
VERSION     DASUGB010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223774"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV87_14590"
     CDS             <1..342
                     /locus_tag="VFV87_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105043.1"
                     /translation="MFILLTAISAIFAVLALALRQPLHWLGLLGVIVVCLAVIGVLEL
                     LCKVFPPKPRFIYYLPEPARPAHPLQTLDFTDGDSPFAPPVPGGESPFASRPTEPPSE
                     LPPRSPPPASR"
     gene            complement(287..1111)
                     /locus_tag="VFV87_14595"
     CDS             complement(287..1111)
                     /locus_tag="VFV87_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5105044.1"
                     /translation="MLRAAGSLVSFVVLLAALPDPVAPDPESKRPIEAVDNVFIEDLT
                     WMEVRDALRAGKTTVLVPTGGVEQNGPYLVTGKHNVVLRGTCEAIARKLGNCLVAPIV
                     PFVPEGDIDPPTVHMKYPGTISLREETFQALLTDICASLKTHGFRNIVLIGDSGGNQA
                     GLKAVAEHLSREWPADKCRVVYIPEYYDLAPVKKLLVSEGIKEVPEGLHDDFAMEATM
                     LAVDPESVRMQQRIAAGNFRINGIELAPADKSIAWGKRIIDLRAEETAAAIRKAVP"
     gene            complement(1157..>2942)
                     /locus_tag="VFV87_14600"
     CDS             complement(1157..>2942)
                     /locus_tag="VFV87_14600"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5105045.1"
                     /translation="AQQPLVPKLSGLVLEYRIVQLYSRDAGERAADLSAEYVVRGAEP
                     PRRADAPRGPGEVVTRGNSVNLAFDCAASHDVTFRVADENAKPTTAAFLIRDKQGRVY
                     PSQAKRLAPDFAFQPQIYRADGEKLKLPAGTYTVVCSRGPESIPETKTLSVGDDGAIF
                     NYQVQRWVDPTLLGWYSGDHHIHAAGCAHYSNPTQGVHAEDMIRHCLGEDLKVGCNLT
                     WGPCFDYQLQFFTGKDDEVSRFPYLLRYDIEVSGFGSHRSGHLCLLRLKERNYPGGES
                     NTHWPTLGLNTLRWAKKQGAITGPAHSGSGLAPTDQRVAEGDGPNGLPNYAIPQYNGI
                     GANEYIVDITHEVPGPDGKPVPAIDFISTMDTNRQQELNMWYHTLNCGFRVRASGETD
                     FPCISGQRVGMGRVYVKVHDKLNYEDYCEGIRDGRSYVSDGTSHLIDFVATSGNQAVP
                     VGTSGSEMKLAAPGKVRLTCKVAARLDGKTVPVEAVVNAYPVAKQEIVADGSLQEISL
                     DVPVERSSWVALRLYPSAHTNPVFVLVDGQPIRASKRSAEWCLAGVDQCWKEKQRTYA
                     ESEQEEAARAYDHAREVYRKIVSESDVP"
BASE COUNT          480 a          934 c          947 g          581 t
ORIGIN      
        1 atgttcatcc tgctcacggc gattagcgcg atcttcgcgg tgctggcgct cgcccttcgt
       61 cagccactgc actggctggg tctcttgggc gtcattgtcg tctgccttgc cgtcattggt
      121 gttctggagc tgctctgcaa agtcttcccg cccaagccgc ggtttatcta ttacctgcca
      181 gaaccggctc ggccggccca tccgctgcag acgctggact tcacggatgg cgacagccca
      241 ttcgcccccc cggttcctgg tggcgagtcg ccgtttgcat cgcggcctac ggaaccgcct
      301 tccgaattgc cgccgcggtc tcctccgccc gcaagtcgat gatccgcttg ccccaggcga
      361 tcgacttgtc ggccggcgcc agttcgatgc cgttgatccg gaagttgcca gcagcgatac
      421 gttgctgcat gcgcaccgac tccggatcga cggccagcat tgtggcttcc atggcgaagt
      481 catcgtgcag cccctcggga acttccttga tcccttcgct tacgagcagc ttcttgaccg
      541 gcgccaggtc gtagtactcc gggatataaa cgacgcggca cttatccgcg ggccattcgc
      601 ggctgaggtg ctcggcgacg gctttgaggc cggcctgatt gccgccgctg tcgccgatca
      661 gcacgatgtt tcgaaagccg tgcgttttca gcgacgcgca aatgtccgtc agcagtgctt
      721 gaaacgtctc ttcgcgaagg ctgatcgtgc cggggtactt catgtgaaca gtcggggggt
      781 cgatgtcgcc ttctggcacg aacggcacga tcggggcaac gaggcaattg ccgagtttcc
      841 gggcgatcgc ttcgcacgtg ccgcggagga cgacattgtg cttgccggtc accaggtacg
      901 gcccgttctg ctcgacgccg cccgtcggca caagcacagt cgttttgccg gctcgcaggg
      961 cgtcgcggac ttccatccag gtcaggtcct cgatgaagac gttatcgacc gcctcgatcg
     1021 gccgcttcga ttccgggtcg ggcgcaaccg gatcgggcaa cgcggcgagc aaaacgacga
     1081 acgacaccaa cgagccagcg gcgcggagca tggaaaggcc tttcaagttg tgggacagaa
     1141 tttgttcgcc aggttgctac ggcacgtcgc tttcgctcac gatcttccga tagacctcgc
     1201 gagcgtggtc gtaggcccgc gccgcttctt cctgctcgga ctcagcatac gtccgctgct
     1261 tttctttcca gcactgatcg acgcccgcca ggcaccactc ggcgctccgc ttgctggcgc
     1321 ggatcggctg gccatcgacg agcacgaaga cggggttggt atgagccgac ggatagagcc
     1381 gcagggcgac ccaactgctg cgctcgacag gcacatccag cgagatttcc tgcagcgagc
     1441 cgtcggcgac gatctcctgt ttggcgaccg gataggcgtt gacgaccgct tcgaccggca
     1501 cggttttgcc gtccaggcgg gccgcgacct tgcacgtcag ccgcaccttg cccggagcgg
     1561 ccagcttcat ttcgctgccg ctagtgccga cgggcaccgc ttgattgccg ctggtggcga
     1621 caaagtcgat caggtggctc gtgccgtcgc tgacgtagct gcggccgtcg cggattcctt
     1681 cgcaatagtc ctcgtaattg agtttgtcgt ggaccttgac atagacccgg cccatgccga
     1741 cccgctggcc ggagatgcag ggaaaatcgg tctcgccgct ggcccgcacg cggaacccgc
     1801 agttcaacgt gtgataccac atgttgagtt cctgctggcg gttggtgtcc atcgtgctga
     1861 taaagtcgat cgccggcacc ggcttgccgt ccggcccggg cacttcgtgc gtgatatcga
     1921 cgatgtactc attcgccccg atgccgttgt attgcggaat ggcgtagttc ggcaggccat
     1981 tgggaccgtc gccttccgcc acgcgctgat cagtgggcgc gaggccggaa ccagaatggg
     2041 ccgggccggt gatcgcgcct tgcttcttgg cccagcgaag cgtattgagg ccgagcgtcg
     2101 gccagtgcgt gttcgattcg ccgccgggat agttccgctc cttcagccgc agcaggcaca
     2161 agtgccccga gcgatgcgac ccgaagccgg agacttcgat gtcgtaccgc agcaggtacg
     2221 gaaaccgcga gacctcgtcg tccttgccgg tgaagaactg cagttgatag tcgaaacacg
     2281 ggccccacgt caggttgcag ccgaccttca gatcctcgcc caggcagtgg cggatcatgt
     2341 cctccgcgtg gacgccttgc gtcgggttcg aatagtgagc acaaccggcg gcgtgaatgt
     2401 gatggtcgcc cgaataccaa cccaggagcg tcggatcgac ccagcgctgg acctggtagt
     2461 tgaagatcgc tccgtcgtcg ccgacggaca gcgttttcgt ctcgggaatc gactccggcc
     2521 cgcgcgagca tacgaccgtg taggttccgg ccggcagctt cagcttctcg ccgtcggcgc
     2581 gatagatctg cggctggaag gcaaaatcgg gagcgaggcg cttggcctgc gaaggataaa
     2641 cgcggccctg cttatcgcga atgaggaagg cggcggtggt tggcttggcg ttctcgtcgg
     2701 cgacgcgaaa ggtcacgtcg tgcgaggcgg cacaatcgaa tgcaaggttg acgctgttgc
     2761 cgcgtgtgac gacctcgccg gggccgcgcg gagcatcggc ccggcgcggc ggttcggcgc
     2821 cgcggacgac atattctgcg gacagatcgg ccgcgcgctc gccggcatcg cggctgtaaa
     2881 gctgcacgat gcggtattcg aggaccaggc cgctgagctt gggcaccagc ggctgctggg
     2941 cg
//
LOCUS       DASUGB010000758         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1056557, whole genome shotgun sequence.
ACCESSION   DASUGB010000758 DASUGB010000000
VERSION     DASUGB010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056557"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFV87_14605"
     CDS             <1..1147
                     /locus_tag="VFV87_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5105046.1"
                     /translation="ILKLFELLGQYGSLSSVTLGESATNQPVGKAAEWLEALAQLPAS
                     AKRSYLGRRLCEALESVQQRGSADALVEDLKDLSDLDAGRAQDSYALVRIVIWATPML
                     GFLGTVIGITDALGDLGRELSVPATADGANALETAIPNLLSGLYVAFDTTAIALCFSI
                     GLMFIQFMLDRSETQLLAAVDERASRELAGRFELTGSQADPNVQAIQRMGQAVLRSTE
                     QLVSKQTDLWQQSISAAHEQWQRTSKHTAEQLQVALAAALSQALTQHAAQIGKIEQTS
                     SEQLAQRWEQWQMSLSHNARLLHAQQQEMVKQGEMMTQAMRAAGEVMQLEKALNQNLA
                     ALSGAKNFEDTVMSLAAAIQLLSTRLGQPVATPHVELRAPQVKGRAA"
     gene            1144..3063
                     /locus_tag="VFV87_14610"
     CDS             1144..3063
                     /locus_tag="VFV87_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105047.1"
                     /translation="MRRRARRQLAGVSLFPFLAVLICTMGSLIVLLVLLVQQARLDAH
                     TLVTGKSPDPPPADAEQFQRQIEEAQWRRELLERSRAEKSQELADTRAKLAHLEDHIR
                     RLTVQARELLARAQNIDEGKQLKDDQLATAEAELARMNDEIAKKQVELDEARKKLKSQ
                     EQWFALIPYQGPNGTRRRPIYIECTSRGVILQPEGLVLQPADFDGPLGPHNPLDAALR
                     TIREYLNRTAPGAGEPYPLLVVRPDGVMAYGAARAALKSWDEEFGYELISSEKKLTFG
                     SPDPGLAAALARSVAVARQRQAAMVAAMPRRYQEHEPLTSFAPDAFPDDPREAGAMGG
                     RGAGRAPGNGHGHGMTGTIPGGTGSASGYPLAGSGAGSPGESIFSPARGGNPGNVGNS
                     AVAGGSAGGGPDNPFGEAGRAGGQFGEAGGSGNQSATGPYGNGNTGQPSGGQPGSAQP
                     GQSSSAGGGSTSGSSGPAASSAGGQMAAASSPQSQGQAGGAPSLSLQLGNPHNSSAAG
                     GQQQNGSSNSAAGAGKGRGRDWALPGASRHATAITRPIYVAVLADRLVVVPQRGTDRQ
                     PQQLPISRSMTVAEVDAFVAAVQREMKAWGLAVDNGYWKPVLQMDVAPGAERHFADLQ
                     TALENSGFEIERKQR"
     gene            3060..>3786
                     /locus_tag="VFV87_14615"
     CDS             3060..>3786
                     /locus_tag="VFV87_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105048.1"
                     /translation="MRRRTKEQADAPGQDSFLDVVCNLVGILIVLVMLIAAQAKRGLI
                     AQETARRTQPDSGSPSPAAKEANAATSAATAVESSVRELQNRIQTQNLEIALRQQERD
                     RVQLLVTVAEQRLAEHRAELTGNDRTRYELETQLAAAHRQLADLDRAAASALQRPPEV
                     IEHLPTPMARTVFGTEIHFRLLGGRLAYVPWEETVERMKTDAPSHMQKLRDSPRVELS
                     LPVQSGFGAKYILRRAEVQTPTKI"
BASE COUNT          700 a         1292 c         1238 g          556 t
ORIGIN      
        1 gattttgaag ctgttcgaat tgctcggcca atacggcagc ctgtcgtcgg tcacgctcgg
       61 cgaatccgcg accaaccaac cagtcggcaa agcggcggaa tggctcgagg cgctagccca
      121 gctcccggcc agcgccaagc gaagctacct tggccgccgc ctgtgcgaag ccctcgaatc
      181 ggtccagcag cgcggttcgg ccgatgcact ggtcgaggac ctgaaagacc tttctgatct
      241 cgacgccggc cgcgcgcagg acagctatgc cctcgtgcgg atcgtcatct gggccacgcc
      301 gatgctcggc ttcctcggca cggtcatcgg aattacggac gccctgggcg acctgggccg
      361 cgaactctct gtcccggcca ctgccgacgg ggcgaacgcc ctggaaacgg ccattccgaa
      421 cctgctttcg ggtctgtacg tcgcgttcga cacgacggcc atcgccctgt gcttttcgat
      481 cggcctgatg ttcatccagt tcatgctcga ccgcagcgag acgcagcttc tggccgcggt
      541 cgatgagcgc gccagccgcg agctggccgg ccgcttcgag ctgaccggtt cgcaggccga
      601 tccgaacgtg caggcgatcc agcggatggg ccaggcggtg ctccgctcga ccgagcagct
      661 cgtgagcaag cagaccgatc tctggcagca gtcaatctcc gccgcccacg agcagtggca
      721 gcggaccagc aagcacacgg ccgagcagtt gcaggtcgcg ctggccgccg cgctgtcaca
      781 ggccctgacg cagcacgccg cccagatcgg gaagatcgag caaacctcct ccgagcagct
      841 tgcccagcgg tgggagcagt ggcagatgtc gctctcgcac aacgcccgcc tgctccacgc
      901 ccagcagcag gagatggtca agcagggcga aatgatgacg caggccatgc gggccgctgg
      961 cgaggtgatg cagctcgaga aggccctgaa ccagaacctg gccgccctgt ccggcgcgaa
     1021 gaactttgaa gacaccgtca tgagcctcgc tgcggccatc cagctcctca gcacgcggct
     1081 ggggcagccg gtggcaacgc cgcacgtcga gcttcgcgcc ccgcaggtga aaggccgcgc
     1141 cgcatgaggc gaagagctcg ccgacaactc gcgggcgtct cgctctttcc gttcctcgcg
     1201 gtgctgatct gcaccatggg ctcgctgatc gtgcttctgg tgctgctcgt gcagcaggcc
     1261 cggctcgatg cccacacgct cgtcacgggc aaatcgccag atccgccgcc ggccgatgcc
     1321 gagcagtttc agcggcaaat cgaagaggcc cagtggcggc gcgagctgct cgaacgaagc
     1381 cgggccgaga aatcgcagga gctggccgat acgcgggcga agctcgctca tttggaagat
     1441 cacatccggc ggctcaccgt gcaggctcgc gaattgttgg cgcgggccca gaacatcgac
     1501 gaaggcaagc agctcaaaga cgaccagctt gccactgcag aggccgagct ggcccgcatg
     1561 aacgatgaga tcgcgaaaaa gcaggtcgag ttggacgagg cccgtaagaa gctcaaatcg
     1621 caagaacaat ggttcgcctt gattccgtac caaggcccca atggcacgcg gcggcggccc
     1681 atttacatcg aatgcacgtc tcgtggcgtg attttgcagc cggaggggct cgtgctgcag
     1741 ccggcggact tcgacggccc gttgggcccc cacaatccgc tcgatgccgc cttacgaacg
     1801 attcgcgagt atctgaaccg gacggcgcca ggcgcgggcg agccgtatcc gctcctcgtc
     1861 gtgcggcccg atggcgtgat ggcctacggc gcggcccgcg cggccctcaa gtcgtgggac
     1921 gaagagttcg gctacgagct catcagcagc gagaagaagc tcacgttcgg ttcgcccgat
     1981 ccgggactcg cggccgcgct cgcgcgatcc gttgccgtcg cccgccagcg tcaggcggca
     2041 atggtcgccg cgatgccgcg ccgctatcag gaacatgagc cgctgacgtc gttcgctccg
     2101 gatgcgtttc ccgacgatcc gcgagaagcg ggagcaatgg gcggccgcgg cgccggccgc
     2161 gctccaggca atggacacgg ccacggaatg acggggacga tcccaggcgg caccggctcc
     2221 gcatcaggtt atccactcgc cggaagcgga gcgggaagtc cgggagaaag catcttctcg
     2281 ccagctcgtg gcggcaaccc agggaacgtc ggcaattcgg ccgtggccgg gggatcagcc
     2341 ggcggcggtc cagacaatcc ctttggcgaa gccgggcgag caggcggtca gttcggcgag
     2401 gccggtggat caggcaacca atctgctacc ggaccttacg gcaacggcaa tacgggccag
     2461 ccctcaggtg gccagcctgg cagtgcgcag cccggacagt ccagttctgc tgggggcgga
     2521 agtacaagcg gatcgagcgg tccagcggcg tcatccgccg gcggacagat ggctgcagcc
     2581 agctcgccgc aatcccaggg gcaggccggc ggcgctccca gcctgagctt gcagctcggc
     2641 aatccgcaca attcgtccgc ggcgggcggc cagcagcaga atggctcaag caactctgcc
     2701 gcaggcgccg gcaaaggccg cggccgcgat tgggccttgc ccggcgccag ccggcacgca
     2761 acggcaatca cgcggcccat ttacgtcgcc gtgctcgccg atcgcctggt cgtcgttccg
     2821 cagcgcggca ccgatcggca gccgcagcaa ttgccgatct cgcgctcgat gacggtggcc
     2881 gaagtcgatg cgtttgtcgc cgccgtccag cgcgagatga aagcgtgggg cctggccgtg
     2941 gacaacggct attggaaacc ggtcttgcag atggacgtcg ctcccggcgc cgagcgacac
     3001 tttgccgacc tgcaaacggc gcttgaaaac agcggcttcg aaatcgagag gaagcagcga
     3061 tgagacgccg caccaaagag caggccgacg cccccggcca ggactcgttc ctggacgtgg
     3121 tctgcaacct cgtcggcatt ctgatcgtcc tggtgatgct gatcgccgcc caggccaagc
     3181 gcgggctgat cgcccaggaa acggcccgcc gcacgcagcc cgacagcgga tcgccttcgc
     3241 ccgccgccaa ggaagccaac gcggccacat cggcagcgac cgctgtcgaa agcagcgttc
     3301 gcgagctgca aaaccgtatt caaacgcaga acctggaaat cgccctccgc cagcaggagc
     3361 gcgaccgcgt gcagcttctg gtgacggttg ccgagcagcg cctggccgag caccgtgcgg
     3421 agctgaccgg caacgaccgc acgcgctacg agctggaaac gcagctcgcc gccgcccacc
     3481 gtcagcttgc cgacctcgac cgcgccgccg cctcggctct gcaacggccg cccgaggtca
     3541 tcgagcacct gcccacgccg atggcccgca cggtcttcgg caccgaaatc catttccgcc
     3601 ttcttggcgg ccgactggca tacgttccct gggaagagac cgtcgagcga atgaagaccg
     3661 atgcgccaag tcacatgcaa aaactccgcg attcgccgcg ggtcgagctg tcgcttcccg
     3721 tccagagcgg atttggggcg aagtacatcc tgcggcgggc cgaagtgcag accccgacca
     3781 agatcg
//
LOCUS       DASUGB010000759         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1195550, whole genome shotgun sequence.
ACCESSION   DASUGB010000759 DASUGB010000000
VERSION     DASUGB010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195550"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="VFV87_14620"
     CDS             <1..2255
                     /locus_tag="VFV87_14620"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5105049.1"
                     /translation="SGRLWWSGGGSSVVHNYGWKDGMLEVGDDFPPALKPGEAAPADA
                     PPTGFRTGMCVNQETGTLYSLTILPKGDAKSFAWGDATTDKGLGGGITLYETRTEKPE
                     MKSLSQKCGKRPYDVVLAKNGLLYVSDWADRRVLVVDPKTLKTAGRISVGEHPNQLAL
                     HPKDSRLFVACASSNAVFVIDTATGTVQETIYTPLFPQSPEGSTPDALCIAPDGETLF
                     VANADNNCIAVVDIEHPRKSAVKGFIPTGWYPTAVAVTPSGKQLLVGVGKGNQTSPNK
                     PAKEKLDAELAKAPAEGGNHYFPFPYIGTTLSGALSIVDVPDEKQLAAYTDQVYRNCP
                     YSDKLLTAAPSPRKTAIPTTVGGKSPIKHCIYIIKENRTYDQVLGDMPRGNGDKSLLM
                     FGAEVTPNHHKLASEFVLLDNLYCNGHVSADGHPWSTMAYNTDYIARNWALTYSRRAG
                     IDDDDEGDLSKAPSGYIWDSCKRNGITYRSYGEYGGRVSQPDGTFRMEGRVPGLVGHM
                     SPRYGLPRKSGERVRDTDRVEVFLEEFREYEKNGNLPRFIVMSLGENHTQGTTPGAPT
                     PQACVASNDLALGRLVEAISRSMYWDKIAIFVIEDDAQNGPDHVDAHRTCGLVISPYI
                     RRGHLDSTQYSTCSMLRTMELILGLPPLSQFDASATPMFESFTDQPNLEPYTHEKARI
                     DFNKLNEPTAYGAERSKQMDFTEYDRIDDFELNEILWRAVKGKDAPLPPAVRRAIAYR
                     GIDIERELAK"
BASE COUNT          507 a          771 c          734 g          418 t
ORIGIN      
        1 gcagcgggcg gctctggtgg tcggggggcg gaagctcggt ggtgcataac tacggttgga
       61 aagacggcat gcttgaagta ggcgacgact tcccacccgc tcttaaaccg ggcgaagctg
      121 ctccggcgga tgcgccgccg acgggtttcc gcactggaat gtgcgtgaac caggaaacgg
      181 gaacgctgta ttcgttgaca atccttccca agggggacgc caagtcattt gcttggggag
      241 acgccacgac ggacaagggg ctcggcgggg ggatcacact ctatgagacg cggactgaga
      301 agccggagat gaaatcgctt tctcagaaat gcggcaaacg tccgtacgac gttgtcctcg
      361 cgaagaacgg cctgctctac gtcagcgact gggcggatcg gcgagtgctc gtcgtcgatc
      421 ccaagacact caagaccgcc ggccgtattt ccgttggcga acatcccaat cagctcgccc
      481 tccaccccaa ggacagccgg ctgtttgtgg cctgtgcttc cagcaatgcc gttttcgtca
      541 tcgacacagc aaccggcacg gtgcaggaaa ccatttacac gcccctcttt ccgcaatcgc
      601 cggaagggag cacgccggac gctctttgca tcgcccccga tggcgaaacg ctgttcgtcg
      661 ccaacgccga caacaactgc atcgcggttg tcgatatcga gcatccccgc aagagcgccg
      721 tgaaaggttt catccccacc ggttggtatc cgacagccgt ggctgtcacg cccagtggca
      781 agcagcttct ggtcggcgtt ggcaagggaa accaaacctc gcccaacaag ccggcgaaag
      841 agaagctcga tgcggaactc gcgaaggccc cggcggaagg tggcaaccat tactttccgt
      901 tcccttacat cggcaccacg ctctccggcg ctctctcgat tgtcgatgtt ccggacgaaa
      961 agcagctcgc ggcttacacc gaccaggtct atcgcaactg tccgtactcg gacaagctcc
     1021 tgaccgctgc tccgtcgccg cgcaagaccg cgattccgac gaccgtcggc ggcaaatctc
     1081 ccatcaagca ctgcatctac atcatcaagg agaaccggac ctacgaccag gttctcggcg
     1141 acatgccccg cggcaacgga gacaagagcc tgctgatgtt cggggcggag gtgacgccga
     1201 atcatcacaa actggcgagc gagtttgtgc tgctcgacaa cctgtactgc aacggccacg
     1261 tttccgcaga cggacatccc tggtccacga tggcctacaa caccgactac atcgcccgca
     1321 actgggccct gacgtattcg cggcgggccg gcatcgacga cgatgacgag ggcgacttga
     1381 gcaaggcccc cagtggctac atctgggatt cgtgcaagcg gaacggcatc acgtaccgca
     1441 gctatgggga atatggcggg cgcgtcagcc agccggacgg caccttccgc atggaaggcc
     1501 gcgtgccggg gctcgtgggc cacatgtcgc cgcggtatgg cctgccgcgg aaatctggcg
     1561 agcgggtccg cgacaccgat cgcgtcgagg tcttcctgga ggaattccgc gaatacgaaa
     1621 agaacggcaa cctgccgcgg ttcatcgtaa tgagcctggg cgagaaccac acgcagggaa
     1681 cgacgcccgg agcgccgacc ccgcaggcct gtgtcgctag caacgacctg gccctcggcc
     1741 gcctggtcga agcgatcagt cgcagcatgt actgggacaa aatcgcgatt ttcgtgatcg
     1801 aggacgacgc gcagaacggc ccggaccacg tcgatgccca ccgcacgtgc ggcctggtga
     1861 tcagccccta cattcgccgc ggccacctcg acagcacgca gtacagtacc tgcagcatgc
     1921 tgcggacgat ggaattgatt ctcggcctgc cgcccctctc gcaatttgac gcttcggcta
     1981 cgccgatgtt cgagtcgttc accgatcagc cgaacctgga gccctatacg cacgagaagg
     2041 cccggatcga cttcaacaaa ctgaacgagc cgaccgccta cggcgcggag cgctcgaagc
     2101 agatggattt cacggaatac gaccggatcg acgattttga gctcaacgag atcctgtggc
     2161 gggccgtcaa aggcaaagac gcgccgctcc cgcccgctgt gcggcgggcg atcgcctatc
     2221 gcgggatcga catcgagcgc gagctggcca agtaaggcta ttccactacc gcggcaaaat
     2281 cctcggccag ccgctccaga acttcgcggg tgacgtgggc ggggacgatt tggcgaagtt
     2341 ggcccgattt tttgctgtag cgactgagag agatatcgcc gccccggcgg acgaggagct
     2401 cataatagct ggttgcttct tcgtccttct
//
LOCUS       DASUGB010000760         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1056806, whole genome shotgun sequence.
ACCESSION   DASUGB010000760 DASUGB010000000
VERSION     DASUGB010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056806"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1571
                     /locus_tag="VFV87_14625"
     CDS             222..1571
                     /locus_tag="VFV87_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5105050.1"
                     /translation="MRVRSSALVVAFFLALAALARAADRPHIVCIMADDLGWMDLHCQ
                     GNPVLRTPHLDGLAKQGVRFTCAYAAAPVCSPTRAALITGQAPARLHITQHGADGKQF
                     WPEDRSVQPPPSKHELAHATTTLAERLKATGYATGFFGKWHLGDDKRFWPTEHGFDVN
                     LGGCGLGGPPTYFDPYRIPSLPPRKAGEYLTDRLADEAIAFMRREKDQPMFVCLWTYN
                     PHYPFEAPEDLIAQFQGQEGAGLKNPIYGGQIEATDCAIGRVLEELDRLNIAEETLVI
                     FTSDNGGWSGATDNRPLRAGKGYLYEGGLRVPLIIRWPGVTHAGAESETPVVSMDLTA
                     TMLDAAGIELRRDERLDGESVRPLFTGSGLQRHALFFHYPHFAFHKANRPGSAIRLDQ
                     YKLVLNYDDDSVELYDLANDVGEQRNLTDALPDVARKMKARLVSWLDETEAGLPEKR"
     gene            complement(1607..3037)
                     /locus_tag="VFV87_14630"
     CDS             complement(1607..3037)
                     /locus_tag="VFV87_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105051.1"
                     /translation="MSPSVRAAELTACSRRALLRDACCGFGGLAFASLLGEEIARAAP
                     ANPLAARQPHQPAKARSVIFLFMAGGPSHLETFDPKPLLNELHGQKRPAEFGEAKYQF
                     IDNDSRLLGTKRKFQKWGESGIEVSDLFPHTAKCIDDIAIVRSCYGDMVVHSAAQYEL
                     FSGRTVPGFPSMGSWTIYGLGAESDSLPAYVVMPDPKGALEAGQPMYMHGFLPAVFQP
                     TMFRPGERPVLNLDLPPGASPQQRRKTLNLIRNLNEAALDPADEELSARLSAYDLAFR
                     MQTEAPEVLDLSRESQQTLDMYGIGKEPTNDYGRRCLLARRLVEQGVRFVCVVAGGGP
                     GNMQWDAHDDIEENHLRKASETDQPVAALLTDLKRRGLLESTLVLWGGEFGRSPEAQS
                     RGGRDHHNLGFSMWLAGGGVQGGQTVGATDAIGLKAIEKPYHFRDLHATILHQLGLDQ
                     HRLTYPHLGRNERLTFVEGKLIEEIL"
BASE COUNT          598 a          979 c         1016 g          562 t
ORIGIN      
        1 ttcagccgat tgagcagtcg attcaaactg gactcccctg cggcgcaaac cggagcgatt
       61 gaaaacccat gcgaccggat cgtactgatg gcgtcagatg ctgtgtctct ggtcggggct
      121 atcctcggcc ctccgatgac acatgcgcat tgcgcatccg acgttcacaa tcggcacgtt
      181 actcaaacca gcaagcggcg gtgattcgtg agggacggcg agtgcgcgtt cgctcttcgg
      241 cacttgtcgt tgcatttttc cttgccttgg ccgcgctcgc ccgcgccgca gatcggccac
      301 acatcgtctg catcatggcg gacgacctgg gatggatgga cttgcactgc caaggcaacc
      361 cagtgctaag gactccgcat ctcgatggtc tggcaaaaca gggagtgcga ttcacgtgtg
      421 cgtatgccgc ggcgccggtc tgctcgccga cgcgggcggc attgatcacc ggtcaggcgc
      481 ccgcacggct gcacatcacg cagcacgggg ccgacggcaa gcagttctgg ccggaagacc
      541 gcagcgtaca gccgccgcca agcaagcatg aactggcgca cgctacgacg acgctcgctg
      601 agcgcctcaa agcgacgggc tacgccaccg gcttcttcgg caaatggcat ctgggcgacg
      661 ataagcgatt ctggcccaca gagcatggct tcgacgtcaa tctcggcgga tgcggattgg
      721 gaggaccgcc cacgtatttc gatccgtatc gcattccgtc gctgccgccg cgtaaggcag
      781 gcgaatacct gaccgatcgc ctggccgacg aggccatcgc gttcatgcgc cgcgaaaaag
      841 accagccgat gttcgtctgc ctgtggacct acaacccgca ctacccgttt gaggcgcccg
      901 aggacctgat cgcccaattc caaggccagg agggagcggg gctgaaaaac ccgatttacg
      961 gcggacagat tgaagcgacc gactgtgcga tcggccgcgt gctggaagag cttgaccgcc
     1021 tgaacatcgc ggaagaaacc ctcgttatct tcaccagcga caatggcggc tggtctggcg
     1081 cgaccgacaa tcgccccctg cgggccggca agggctatct gtacgaaggc gggctgcgcg
     1141 tgccgttgat catccgctgg ccgggcgtga cccacgcggg cgcagagagc gagacgccag
     1201 tggtgagcat ggacttaacc gcgacgatgc tcgacgccgc cggcatcgag ctgcggcgtg
     1261 acgagcgtct cgacggcgaa tcggtgcgtc cattattcac gggaagtggc ctgcagcgcc
     1321 acgcactgtt tttccattat ccacactttg ccttccacaa agccaatcga ccgggctcag
     1381 ccattcgcct tgaccaatac aagctcgtcc tgaactacga cgacgactcg gtggagctgt
     1441 acgatttggc aaacgacgtg ggcgaacagc gaaaccttac tgatgccttg cccgacgttg
     1501 cgcggaaaat gaaagctcgt ctggtaagct ggctggatga gaccgaggcg ggactgccgg
     1561 agaagcgctg atcccacgtg ccgcgtgtcg acactcgggt tgggggctag agaatctctt
     1621 cgatcaactt gccttcgacg aacgttagcc gttcattgcg gcccaggtgc ggataggtga
     1681 gccggtgctg gtcgaggccg agttgatgga ggatggtggc gtgcaggtcg cggaaatgat
     1741 agggcttttc gatggctttc aggccaatgg cgtcggtcgc gccgacagtc tggccccctt
     1801 gcacgccgcc gccggccagc cacatcgaga aaccgaggtt gtggtggtcg cgtccgccgc
     1861 gggactgcgc ctcgggcgaa cggccgaatt cgccccccca caagacgagc gtgctttcca
     1921 ggaggccgcg gcgcttcaga tcggtgagca gcgcggcgac cggctggtcg gtttccgacg
     1981 ccttccgcag gtggttctcc tcgatgtcgt cgtgggcatc ccactgcatg ttgcccggcc
     2041 cgccgccggc gacgacgcac acgaaccgca ccccctgctc gaccaagcgc cgcgccaaaa
     2101 ggcaccgccg gccatagtcg ttcgtgggct ccttgccaat gccgtacatg tcgagcgttt
     2161 gttgcgattc gcgcgacaga tcgagcactt ccggtgcttc ggtctgcatt cggaaggcga
     2221 gatcatacgc gctgaggcgg gctgagagct cttcatcagc cggatccagt gcggcttcgt
     2281 tcaagttgcg gatcaggttg agtgtctttc tccgttgctg cgggctggcg cctggcggca
     2341 ggtcgaggtt gagcaccggc cgttcgcccg ggcggaacat cgtcggctga aagacggccg
     2401 gcagaaagcc gtgcatgtac atcggctggc cggcttcgag ggcccctttc ggatcgggca
     2461 tgacgacgta cgccggcagc gaatcgctct cagcccccag gccgtagatc gtccacgagc
     2521 ccatgctggg aaagcccggc acggtgcggc cggagaaaag ctcgtactgg gctgcggaat
     2581 ggacgaccat gtcgccatag cacgagcgga cgatggcgat gtcgtcgatg cacttcgcgg
     2641 tatgcggaaa caggtccgaa acttcgatcc cgctttcgcc ccacttctgg aacttgcgct
     2701 tcgtccccag cagccgcgag tcgttgtcga tgaactgata cttcgcctcg ccaaactctg
     2761 ccggccgctt ctggccgtgc agctcgttaa ggagcggctt gggatcgaag gtctccaagt
     2821 ggctcggtcc gcccgccatg aacaggaaaa tgaccgatct ggccttggcc ggctggtgcg
     2881 gctgcctggc ggcgagcgga ttggccggag cagcccgggc gatctcctcg cccaggagcg
     2941 atgcaaacgc cagcccgcca aacccgcaac aggcgtcgcg cagcagtgcg cggcgcgagc
     3001 aggcggtcaa ttcggcagcg cgaacgctag gggacataag cgaattcatt cagattgaat
     3061 agagccagac aaaattcagt aagcggctga tcgcggagga aagcgagcga caggtcgcgc
     3121 tcctgagcgg tcggggcgcg ggcaaatgcc agaag
//
LOCUS       DASUGB010000761         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_362191, whole genome shotgun sequence.
ACCESSION   DASUGB010000761 DASUGB010000000
VERSION     DASUGB010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362191"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VFV87_14635"
     CDS             complement(<1..1671)
                     /locus_tag="VFV87_14635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105052.1"
                     /translation="MTSRNLRCPTPQPDEAQASSVNKGPTANSAGDTSLLRLLSARVD
                     LSPGSQLGTYKILAEIGRGGMGTVFRGFHTRLHKHVAIKVLPTSQSESREGIERFERE
                     MEIVGQLQHPHIVVAHDGGEIEGLHYLVMELIEGENLEQIARVRNRLSICDSCALIQQ
                     AAAGLHYAHSRGLVHRDVKPSNLMLDRNGIVKILDLGLARWSAPHLGISDLTASGHWL
                     GTLLYMAPEQAENAHGVGAAADLYSLGATLYRLLSGRAPIGDPASNPVQLLSVLMHGS
                     IEPLERHRPECPAELAALVHQMLARTPADRPGSAAEVAERLGAFSAGADLKWLLPSSS
                     GDSGEAAASSHSRAELPASDPVNDDLSTPLHADRASNADASSIMQRSDVKIRRLRLIV
                     CAIAGLALAAAGLITLWSSGILAPEPQAILRVSYPGDTAPEVRIDGQLVAADTRTPGM
                     LVIDVAPGVHKLSIEAAGATWRSNTGDRPLTFEDGETIDVESWPAATPEDTARWVIEF
                     GGYVATLDAEGGVRFVNSAEPALPSKPYVITRVWLPDTNIADDDLRRLA"
     gene            complement(2351..2959)
                     /locus_tag="VFV87_14640"
     CDS             complement(2351..2959)
                     /locus_tag="VFV87_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105053.1"
                     /translation="MKKVTFHGLLASLAIGCLVFSSMPVRASNPAFGGLLTYGANGGN
                     CPNSGGSATPSPDGVGGGFGGGGGFGGGGGFGDGGGFGGGGGFGGGGGFGGGDGFGVG
                     GGGGFGGGTPNCPPPDVWCPPQPPCPPQRPPCPPWTWSPPPQSPPTCQYPPTTWQQQY
                     GKQYGKRPSLPAPPIPGQQQSQHVRPYSGYPYPYLPPAVAIR"
     gene            complement(3228..>3882)
                     /locus_tag="VFV87_14645"
     CDS             complement(3228..>3882)
                     /locus_tag="VFV87_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5105054.1"
                     /translation="ARSEILAVRDSFERAFPATDAVTVLRQAHASEARIRQECSRHPF
                     IHIVTHGFVAESTPAPLAPGLSAGLALAGANEPGDDPLDDGILSAAEFADLDLRAAEL
                     VVLSACESGLGPIAAGEGVLGLQRACQMAGAKTVVASLWRVNDRATQQLMEMYYERVC
                     ATADPISRIEALRQAQLAMLRGGGQRGLGIVSRAPARATERRAPYEWAAFVLGGDWR"
BASE COUNT          753 a         1240 c         1193 g          696 t
ORIGIN      
        1 cgccaggcgc cggaggtcgt cgtcggcgat gttggtatct ggcagccaga ccctggtgat
       61 gacatagggc ttactgggga gcgcgggctc tgccgagttc acaaatcgca cgccaccttc
      121 cgcatcgagc gtggcaacgt aacctccgaa ttcaattacc cagcgggccg tatcttcagg
      181 agttgccgcc ggccaggatt ccacgtcaat tgtttcgccg tcctcgaacg taagcggtcg
      241 atcgcccgtg ttgcttcgcc atgtcgctcc tgcggcctca atcgatagct tgtgtacgcc
      301 cggcgccacg tcgatcacca gcatgcccgg agtcctcgta tcggcagcga ccaattgccc
      361 atcgattcgc acctcaggcg cagtgtctcc gggatagctc acccgcagga tcgcctgcgg
      421 ctccggcgcg aggattccgc tgctccatag ggtgataagt ccggcggcgg caagtgccag
      481 gccggcgatc gcgcatacaa tgagccttag tcgccgaatc ttgacgtcgc tacgctgcat
      541 tatcgatgaa gcgtccgcat tcgaagctcg atcagcatgg agcggtgtgg acaggtcgtc
      601 gtttacaggg tcactcgcgg gcagttctgc tcgtgagtgc gagctagccg ctgcctcgcc
      661 gctgtcgccc gagctcgatg ggagcagcca cttcagatcc gccccggctg aaaacgcgcc
      721 caaacgttca gcgacctccg ccgccgaacc aggccgatcg gcaggagttc gggcgagcat
      781 ttgatggacg agtgcagcca actcggcagg gcactccggc cgatggcgct caagcggctc
      841 gatgctgcca tgcatgagaa cggaaagcag ctgaaccggg ttggatgccg gatcgccgat
      901 gggtgcgcga ccgctcaaca gccggtagag ggtcgcgccc agggaataca ggtcggcggc
      961 agcgccgacg ccgtgcgcgt tttcggcctg ttccggcgcc atgtatagga gagtccccag
     1021 ccagtgtccg gaggccgtca gatcggagat tcccaggtga ggcgccgacc agcgcgccaa
     1081 gcccaggtcg aggattttga cgatgccgtt gcggtcgagc atcagattgg agggtttaac
     1141 gtcgcgatga accaacccgc gggaatgggc atagtgcaat cccgcggccg cttgttgaat
     1201 cagagcgcac gagtcgcaaa tcgacaagcg attccgcaca cgagcaatct gctcgaggtt
     1261 ttcgccttca atcaattcca tcaccaggta gtgcagcccc tcgatttctc ccccatcgtg
     1321 ggcgacgaca atatggggat gctgcaactg gccgacgatc tccatttcgc gctcgaatcg
     1381 ctcgatgccc tcccggcttt ccgactggct cgtcgggagg accttgatgg ccacgtgctt
     1441 gtggagcctg gtgtgaaacc cccgaaacac cgtccccatt ccgccgcggc cgatttccgc
     1501 gagaattttg tacgttccca gttggcttcc tgggctcagg tcaactcgcg ccgaaagtag
     1561 cctcagaagt gacgtatcgc ctgcagagtt ggctgtcggt cctttattca ccgaactggc
     1621 ttgcgcctca tcgggttgcg gagtcgggca cctcaggttg cgtgaagtca tcggatgacg
     1681 ttcttgcttc gcgctgctct agaacgataa tcccattcat tcttagcctt atgggaccat
     1741 agccgcaagt ttcctgccgt cctccccccc gtcctcccca agagtgccca aacgccaagc
     1801 tagccaaggg ccaggaacga cgacgccaaa ggctcgccaa cgaaaatccg gtgggtccag
     1861 cgccgcagtg tatgtcaatc gcgtctcgcg ttgttgcgag tgatccggcc gctgtaaatg
     1921 aaccgcgacg cgcggcggaa acgcaaaact cggcggggcg acgcgaaggg gcggcgtgcc
     1981 gattccggaa aagggcgggg ggccgccggc cgacgagcgc tatcgcccat atggccgcag
     2041 aggactcgac ttgtggcgcg ccaagtccag cgagccgacg ccggctgatc ttcatccatg
     2101 acggcagctt ggcagtggac aagcgcggct actggaccaa cctggtcact cgccgcttgc
     2161 atttcgggcg gcaatggccg aatacgtcgc gccaggccga tgcacttgga atctcgtgaa
     2221 gagtctagcc gggaggccgc atgtgacgag ccatggcgac acgtatccct acaatgaagg
     2281 aagaacgcca agagcggtct cttggcgttc caaggaagag agtcagtcga agcttcccac
     2341 aatgcaaggt ttaccggatg gcgacagcgg gaggcaggta gggataggga tagccgctgt
     2401 aaggcctgac gtgctgcgac tgttgctggc cagggatcgg aggggcgggc aacgaggggc
     2461 gcttgccgta ttgcttaccg tactgctgtt gccaggtcgt cggaggatac tggcacgtcg
     2521 gcggactctg cggcggcggc gaccaggtcc acggcgggca gggcggacgc tgaggcgggc
     2581 acggcggctg cggcgggcac caaacatcgg gaggagggca attgggcgtt ccgccaccaa
     2641 agccacctcc acccccgacg ccgaagccat cgcctcctcc aaaaccgcca ccgccaccaa
     2701 agcctccacc gccgccaaat ccaccgccat caccgaaacc gccgcctccg ccgaagcccc
     2761 cgccaccgcc gaagccgccg ccaacgccat cgggactcgg cgtagcgctc cctccgctgt
     2821 tggggcagtt gccgccattg gcgccgtaag tcagcagtcc gccaaaagca ggattgctgg
     2881 ccctgacggg catggagctg aaaacaaggc aaccgatcgc gagcgaagcc agcaatccgt
     2941 ggaaagtgac cttcttcatt ttggaactcc ttaggaaagt cgggtgaatt tcgtcactga
     3001 ccttacatgg ccgtcgcttc catccgcccg acactttttt tgcctgcatt gcagagacgt
     3061 tgcttgggaa cgacgacatc gttcccggaa ccaagcgcgg cgagagaacg agacgcttca
     3121 ctagtgcttt gcaatcacaa gagttgcaac ggcgacagat cagattcctg cggatgcgaa
     3181 gtcggcgctg caatccgttg ccccaggcgg ttatgaagca gcaacgctta tcgccagtcg
     3241 ccgccgagga cgaatgccgc ccattcgtaa ggcgctcgtc gctcggtcgc tcgtgcgggt
     3301 gcgcgggaaa cgatccccaa cccgcgctgc ccaccgccgc gcagcattgc gagttgggct
     3361 tgtcgtaacg cttcgatgcg actgatggga tcagccgttg cacagacgcg ctcgtaatac
     3421 atttccatca attgctgcgt agcccggtcg ttcactctcc acaagctcgc gacgacggtt
     3481 ttggcgcccg ccatctggca ggcgcgctga aggcccagaa cgccttcgcc tgctgcaatc
     3541 gggcccagcc ccgattcgca cgccgaaagc acgaccaact cagccgctcg caaatccagg
     3601 tctgcgaact ccgcggctga gagaatcccg tcgtcgagcg ggtcgtcgcc cggttcgttc
     3661 gcgccagcca gggccaaacc ggccgacaat ccgggcgcca gtggtgctgg ggtggattcg
     3721 gccacgaatc cgtgcgtcac gatgtgaatg aatggatgcc gagaacattc ctgacgaatg
     3781 cgtgcctcgc tggcatgcgc ctgccgcagg accgtcacgg catccgtggc aggaaacgcc
     3841 cgctcgaagg aatcgcgcac cgccagaatc tcgctccgcg cc
//
LOCUS       DASUGB010000762         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1613810, whole genome shotgun sequence.
ACCESSION   DASUGB010000762 DASUGB010000000
VERSION     DASUGB010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613810"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFV87_14650"
     CDS             complement(<1..419)
                     /locus_tag="VFV87_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5105055.1"
                     /translation="MPSTLIVPNRRLFLGALGASLFTTRGLFAELLQTPAQTEGPFYP
                     DKLPLDTDNDLLVINDKLTPAVGQITHVTGKILDAKGNPLKNAVVEIWQCDGNGVYLH
                     SGSDNGGKRDGNFQGFGRFLTGSTGEYYFRTIKPVPYP"
     gene            complement(731..1144)
                     /locus_tag="VFV87_14655"
     CDS             complement(731..1144)
                     /locus_tag="VFV87_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105056.1"
                     /translation="MHGRSEWTKSTTMPAYYLFSPQLPAFVMAFRSPSQMAQYMVSND
                     YEPQHWIDNSFVVGPEQLRGFVDYDKTGFFVDWTDETVPPEELREVLTKRLSVIGWRR
                     EQFTASVILVRNFDDFKRVYAQFPDRSERAGWIRG"
     gene            1225..2586
                     /locus_tag="VFV87_14660"
     CDS             1225..2586
                     /locus_tag="VFV87_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5105057.1"
                     /translation="MPRNVHSRRQFITTTAAGVVALSVPAIGRAQNTNSRINLAFIGV
                     GGRGGANLKEMTKTASENVVALCDVNGNNLDAMGERYPQARKYRDFRKLYDEAKDIDA
                     VVVSTCEHTHAYATLPALQMGKPVYCEKPLTLNVAEARLVSQAAAKAKVPTQMGTQIH
                     ASPNYHRVVELIQSGAIGKVSEAHVWVSRAWGRQSKEEAEANKDIVFVTERPAEAQDP
                     PPYLDWDLWIGPAPYRPFHSVYFPGPKWYRWWDFGNGTMSDLGSHWNDLPFWALKLDA
                     PQTIEATGPEPHPEIAPASMTAVYEYGERDGGKLPPVRLTWYQGTYKPPQWQENPAVR
                     AFGSGVLFVGEGGKMLVSDYSRHVLLPEADFKDFQRPEPFVPDSPGQQLEWLAAIRNG
                     TPTGSPFHPYAGLLTEANHLGNVAYRAGQKIHWDSANLRVTNTRAADPYLSREPREGW
                     KLG"
     gene            complement(2610..>3679)
                     /locus_tag="VFV87_14665"
     CDS             complement(2610..>3679)
                     /locus_tag="VFV87_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5105058.1"
                     /translation="MLDLEETIALVVGKTEHAATSQTNVLKNALLEARQEGAQAIGIS
                     AGEITVDSPIPYELGDPAGLDVFGKRNGTQYLTGLIGKINGQRPENQNKKDHEEFNKL
                     NRKIESLRRDVRLRFMMQPWERASNDPLPGILGQLFGKGGAVCVVDLSGVPNEVAGSA
                     SSAVARTIFAVKLWQTPEERVRNPVVLVCEEAHRYVPDRGEAQYAAAREAVQRLAKEG
                     RKYGIGLALVSQRPSEVDPTVLSQCNSWIVLRITNESDREHVRAILPDSLVGLTKVLS
                     GLRQREAIVVGQAITLPSRILIRRLEQSSLPRSHDISFTKGWQSDYLDEASLSQIAAR
                     WRFQNRSGAPAEDDDAQTSPF"
BASE COUNT          768 a         1124 c         1094 g          693 t
ORIGIN      
        1 ggatagggca ccggcttgat cgtgcggaag taatattcgc ccgtgctgcc ggtaaggaag
       61 cggccgaagc cctggaaatt gccgtcgcgt ttgccgccgt tgtcgctccc gctgtgcaga
      121 tagacgccgt tgccgtcgca ctgccagatt tcgaccaccg cgttcttcag cggattgccc
      181 ttggcatcga gaattttgcc cgtcacgtgc gtgatctggc ccaccgccgg cgtgagctta
      241 tcgttgatca cgagcaggtc gttatccgta tccagcggca gcttgtcggg ataaaacggc
      301 ccctcggtct gagccggcgt ctgaagcagc tcagcaaaca agccgcgggt cgtaaacagc
      361 gacgcgccga gcgcgccgag aaacaagcgg cggttgggaa cgatcaaggt cgatggcatg
      421 acggcagcct tgctgacgag agaaggggac tagggtgaaa caccagcctg atgggaatgt
      481 tccgcgactg tgccgctcgg caattgcaac gcacgacgca gccgcgttat tgcattctcg
      541 caggaagcgg cgttttagga aagcaacgag tttgtaaacg atatggttgg ctatccctaa
      601 tgaagcagaa gaaattgttc gccgtgttgc gcgaggacaa tccatgtccc taatggaaga
      661 tcttgaagat ccctcggaaa ctccacacga cgacgactag tatcacgaac gccacgaacc
      721 caacataaag tcatccgcgg atccaccccg cgcgctcact gcggtccgga aactgggcat
      781 agacgcgttt gaagtcgtcg aagtttcgta cgaggatcac cgacgcggta aattgctcac
      841 gtctccaacc gatcaccgaa agtcgcttcg tgagaacttc ccgaagttct tccggaggaa
      901 cggtttcgtc cgtccaatcg acaaagaatc ccgtcttgtc gtaatcgaca aagccgcgga
      961 gctgctcggg gccaacgacg aaggagttgt cgatccaatg ctgcggctcg tagtcgttgc
     1021 ttaccatgta ttgagccatc tggctcggcg accggaacgc catcacgaat gcaggcaatt
     1081 gcggcgagaa caggtaataa gctggcatcg tcgttgattt ggtccactcg gatcgtccgt
     1141 gcacggaaaa ccacgtgcag tccagatcgg gatcggcgac aatggtggct ggctagattc
     1201 tgttcatgcc tttggaggcc caccatgccg agaaatgtac attcacgccg tcagtttatc
     1261 acaacgaccg ctgcgggcgt tgttgcgctt agcgtccccg caatcggccg ggcgcagaac
     1321 accaacagcc gcatcaacct ggcgttcatc ggcgtgggcg ggcgcggcgg ggcgaatctg
     1381 aaggagatga ccaagacggc gagcgagaac gtcgtcgccc tgtgcgatgt gaacggcaac
     1441 aacctcgatg cgatgggcga gcggtatccg caggcccgaa aatatcgcga tttccgcaag
     1501 ctgtacgacg aagccaagga catcgacgcg gtcgtcgtca gcacgtgcga gcatacgcac
     1561 gcctacgcca cgctgccggc cctgcagatg ggcaagccgg tctattgcga gaagccgctg
     1621 accttgaacg tggccgaggc ccgcctcgtc agccaggcgg cggcgaaggc gaaagtgccg
     1681 acgcagatgg ggacgcagat tcacgccagc cccaactatc accgcgtcgt cgagctgatc
     1741 cagagcggag cgatcggcaa ggtcagcgag gcccacgtct gggtctcgcg ggcctggggc
     1801 cggcagagca aggaagaggc ggaggcgaac aaggacatcg tcttcgtcac cgagcggccg
     1861 gccgaagccc aggacccgcc gccatatctc gattgggatc tgtggatcgg cccggccccg
     1921 taccggccgt tccatagcgt ctatttcccc gggccgaagt ggtatcgctg gtgggatttc
     1981 ggcaacggca cgatgtcgga cctggggagc cactggaacg acctgccctt ctgggccctc
     2041 aagctcgacg ccccgcaaac catcgaagcg accggcccgg agccgcaccc ggaaatcgcc
     2101 ccggcttcga tgacggccgt ctatgaatat ggcgagcggg acgggggcaa gctgccgcca
     2161 gtgaggctca cctggtatca gggcacgtac aagccgccgc agtggcagga gaacccggcg
     2221 gtgcgggcgt tcggcagcgg cgtgctgttc gtcggcgaag ggggcaaaat gctggtctcc
     2281 gactacagcc ggcacgtgct cctgccggag gcggacttca aggacttcca gcgaccggag
     2341 cccttcgttc ccgattcccc cggccagcag ctcgaatggc tggccgccat tcgcaacggc
     2401 acccccaccg gcagcccgtt ccatccctac gcgggcctcc tcaccgaagc caaccacctc
     2461 ggcaacgtcg cctaccgcgc cggccagaag atccactggg acagcgccaa cctccgcgtc
     2521 accaacaccc gcgccgccga cccctacctc agccgcgagc cgcgggaagg ctggaagctg
     2581 gggtaggacg ggctgtgcca accaacgtgt tagaacggtg atgtttgcgc atcatcgtcc
     2641 tcagccggcg caccggaccg attctgaaac cgccatcgtg ccgcgatttg gctcagagac
     2701 gcttcgtcta agtaatcgga ctgccatccc ttcgtgaaac taatatcgtg cgaacgtgga
     2761 aggctgcttt gctcaagtcg gcgaatcaaa atccgcgaag gcagcgtgat agcttggccg
     2821 acgacgatgg cctcgcgctg gcgaaggccg gaaaggacct tcgtcaatcc tacgagcgaa
     2881 tcgggtaata tcgcccggac atgttcgcga tctgattcgt tcgtgatccg aagcacgatc
     2941 cacgaattgc attgcgacaa cacagttggg tccacctcac ttggtcgttg ggataccagg
     3001 gctagcccaa tgccgtactt ccggccttct ttcgccaggc gctgaacggc ctctcgggcg
     3061 gccgcgtact gcgcctctcc gcgatcaggc acataacggt gcgcctcttc gcatacaagc
     3121 acaacaggat ttcgcacccg ttcctctggt gtttgccaaa gtttcactgc aaaaatcgtt
     3181 ctagcgacgg ctgaactcgc cgagccggcg acctcgttcg ggacgccgga taggtctacg
     3241 acacagacag cgcccccctt gccgaaaagc tgtccgagga tacccggcag agggtcgttg
     3301 gaagcccttt cccacggctg catcatgaag cgaaggcgaa catctctacg gagcgactcg
     3361 attttgcgat tcagtttgtt gaactcctcg tggtccttct tgttttggtt ttcggggcgt
     3421 tgaccgttta tcttgccaat cagacctgtc aggtattgcg tgccgtttcg cttgccaaat
     3481 acatcgagcc cagcgggatc gccaagttcg tacggaatcg gggaatcgac cgtgatttca
     3541 ccagcgctaa tgccaatggc ttgggcacct tcctgccgag cctcaagcaa cgcatttttc
     3601 agcacatttg tttgcgaagt cgcggcatgc tcagtctttc cgacaacaag tgcaattgtt
     3661 tcttcgagat cgagcatcc
//
LOCUS       DASUGB010000763         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_918555, whole genome shotgun sequence.
ACCESSION   DASUGB010000763 DASUGB010000000
VERSION     DASUGB010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918555"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..2711)
                     /locus_tag="VFV87_14670"
     CDS             complement(375..2711)
                     /locus_tag="VFV87_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105059.1"
                     /translation="MSRVPDKSRKIIPQSGPQRPAMPPLPAGAPQGKATGGEAPAFTI
                     NVGAPAKGGAGKGSAATYRGGKKKSSNLPVVLAIVAAVLMVGGAGGVAAYVVLNDSRR
                     VEVAQGPKKPQEEPTAEELPTGAGTPAPVSTEGNSAAASAKTGGDDFQFKPMYVADDP
                     PPGEPSPPAAPAPPVSSAPPSPSSSSPSSSPTPASPNPAPPTAPAPASLQPPQATEED
                     AFNQAIVDLHAAGNLLEKKEYPALRKLYADRFARLNEAAIKQGLGADYEPMMAWFAEH
                     PEIQEELYTAIRPEDKVPAVMQLFNELRKQFPEKIVPYANLAIATAVVWDDERRGVYD
                     YGHHASRCKATMPANLLGAIENFQYLVSAEPVMQGRIQYVPWEFLVYVVNHRTPQPER
                     VWAVQNFVGKRVMYGKCYSDVPYDNEMLKSGSEIAKLNNRDYTLPNILQFGGLCAMQA
                     DFASRVGKSMGVAAEYVNGESAGGDRHAWVMWVELKQATPTGLVFSLESHGRYRGDKY
                     YVGNLDDPQTGQNITDRDMELRLQTVGLDTVAKRHAALVMQAYPMLCERASLDATQRL
                     DLLAKTIAYSPGCEEAWYATARFFREASGNKEHAKQYQAVLDRLFATFARVPDFTWKV
                     FDDLAAYQSDAKASSAMYVRLCGLYEAAERPDLACESRLKLADMLVEQAKPLDAVQGL
                     AFTIKKFPSEGRYVPKMLDKIELLLKDVPNAGQHLVGFYVEILPLVPQMRGDEPSPFA
                     ITMFERAVDVFTQCNQPQLAQAAQVELEKIKSGNGKRG"
     gene            complement(2848..3384)
                     /locus_tag="VFV87_14675"
     CDS             complement(2848..3384)
                     /locus_tag="VFV87_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5105060.1"
                     /translation="MTRSRYRICDNACPHFMTCTIVGWLPVFTRPEAVQIVFDSWTWL
                     RANRGFEIYGYVILENHLHLIASSLNLAEDMGDFKSFTARQIIDFLEERRARTLLKQL
                     AWHKARHKVDRDHQLWQEGSHPEEIQSTEMMWQKLEYAHNNPVERGYVALPEHWRHSS
                     AANYLGLPGVFPVRTDWQ"
     gene            complement(3482..>3855)
                     /locus_tag="VFV87_14680"
     CDS             complement(3482..>3855)
                     /locus_tag="VFV87_14680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5105061.1"
                     /translation="PAGIFVAVESTVGNALRGVPLPSPLLGEGPGVKGQNETIVGYIT
                     TWQDREAGIGHIPNIAIRPEYRGQGLGRILIEHALAHFRASGLTHAKIETLAQNAIGN
                     HLYRDLGFIEVARQVHFVAKL"
BASE COUNT          637 a         1297 c         1186 g          735 t
ORIGIN      
        1 ccgcgcaaca tttccgtaag tccaatacag gcatcaggtt ccatcgcata gaccccccat
       61 tcgaggatgt tgcactgttg cgcgcgctcg ctctgccgct ccgcgtggga acacacgcac
      121 cgccgcttcc gaccgatcga cgcagagcgt cgcaagcagg cgttcccacg cggagcgtgg
      181 gaacgagccg ctctcccgct cagcgcgcac cgcctcccca tccattagat gctgtactga
      241 acagatttag tccatccatc tggccggaat ttcaaaaatt cttcccagcc gattttcctt
      301 ggcgcacagc cacgatgtcg ccccgcgacc gccgggggtt gtccagcaag ctcgtcgggg
      361 agggcagcgg cgacctaacc ccgcttccca ttccccgact tgatcttctc cagctcgacc
      421 tgcgccgctt gggcgagctg cggctggttg cactgggtga acacgtccac cgcccgctcg
      481 aacatcgtga tcgcaaacgg gctcggctcg tcgccgcgca tctgcggcac cagcggcaga
      541 atctcgacat agaaacccac caggtgctga ccagcgttcg gcacgtcctt caagagcagc
      601 tcgatcttgt cgagcatctt cggcacatag cggccttcgc tggggaattt cttgatcgtg
      661 aaggccaggc cctgcacggc atcgagcggc ttggcctgct cgacgagcat gtcggccagc
      721 ttcaggcggg attcgcaagc caggtcgggc cgctccgccg cctcgtacag gccgcacagc
      781 cgcacgtaca tcgcgctgct ggccttcgcg tccgattgat aggccgccag gtcgtcgaac
      841 accttccagg tgaagtcggg cacgcgggcg aacgtcgcga acagccggtc gagcaccgcc
      901 tggtactgct tggcatgctc cttgttgccg cttgcctcgc ggaagaaccg ggccgtggcg
      961 taccacgcct cctcgcagcc ggggctatag gcgattgtct tggctaacag gtccagccgc
     1021 tgcgtagcat cgaggctcgc cctctcgcac agcatgggat acgcctgcat caccagggcc
     1081 gcgtgccgtt tggcgaccgt gtcgagaccc accgtctgca gccgcagctc catatcgcgg
     1141 tcggtgatgt tctggccggt ctgcggatcg tcgagattgc cgacgtagta cttgtcgccg
     1201 cgataccgcc cgtgcgattc gagcgagaag accagccccg tcggcgtggc ctgcttgagt
     1261 tcgacccaca tgacccaggc gtgccggtcg ccgccggccg attcgccgtt gacgtattcg
     1321 gccgctaccc ccatgctctt gcccacccgc gaagcaaagt cggcctgcat cgcgcacaag
     1381 ccgccaaact gcagaatgtt cggcagtgtg taatcccggt tgttcagctt cgcgatctct
     1441 gaaccgctct tgagcatttc attgtcgtac ggcacgtccg aatagcactt gccgtacatc
     1501 acccgcttgc cgacgaagtt ctgcaccgcc cacacccgtt ccggctgcgg cgtgcgatgg
     1561 ttcacgacat agaccaggaa ttcccagggg acgtactgaa tccgcccctg catgaccggc
     1621 tcggccgaaa ccaggtactg gaaattctcg atcgccccga gcaggttcgc cggcatcgtc
     1681 gccttgcagc ggctggcgtg atggccgtag tcatacacgc cgcggcgttc gtcgtcccaa
     1741 acgactgctg tggcgatcgc caggttcgcg tacggcacga tcttctcggg aaactgcttc
     1801 cgcagctcgt tgaagagctg catcaccgcg ggcactttgt cctcggggcg gatcgccgtg
     1861 tacagctcct cctggatctc cggatgttcc gcgaaccagg ccatcatcgg ctcatagtcc
     1921 gcgcccaggc cttgcttgat cgcggcctcg ttcaggcggg cgaaccggtc ggcatacagc
     1981 tttcgcaggg cgggatactc cttcttctcc agcagattgc cggcggcgtg cagatcgaca
     2041 atcgcctggt tgaaggcgtc ttcctcggtg gcctgcggcg gctggaggct ggccggcgcg
     2101 ggcgcggtgg gcggcgctgg atttggactg gcgggcgtcg gcgacgatga cgggctggat
     2161 gagcttggac tgggcggcgc gctggaaacg ggcggcgcag gcgctgctgg gggtgatggc
     2221 tcgccgggcg gcggatcgtc ggcgacgtac atcggcttga actgaaagtc gtcgccgcct
     2281 gtcttcgcac tcgctgcggc agagttcccc tctgtgctca ctggcgcagg agtgcctgcg
     2341 cccgttggca actcctcggc agtcggctcc tcctgcggct tcttcgggcc ttgtgcgacc
     2401 tcgaccctcc gactgtcgtt cagcacgaca taagccgcca ccccccctgc cccgccgacc
     2461 atcagcaccg ccgcaacaat cgccagcacc accggcaggt tcgaggactt cttcttcccg
     2521 ccccgatacg tcgccgcact ccccttccct gccccgccct tcgccggcgc gcccacattg
     2581 atcgtaaacg ccggcgcctc gccgccggtc gctttgccct gcggcgcacc cgccggcagc
     2641 ggaggcatcg cgggacgctg cggccccgac tgcgggatga tcttgcggct cttatcggga
     2701 actctggaca cgcgagtttc ctcaccgaat tggaacgaga acgctatctt gaccccccga
     2761 ttatgggtgt cggcacagaa gcgggtcaag ctcgttccca cgctccgcgt gggaacgcac
     2821 gcctcgccgc tccgcggcgc gagccgccta ctgccaatcc gtcctcaccg gaaaaacacc
     2881 cggcaggccg agataattgg ccgcgctcga atgccgccaa tgttccggca gtgccacata
     2941 gcctcgctcg accggattgt tgtgcgcgta ctcgagtttc tgccacatca tctccgtcga
     3001 ttgaatctct tcggggtggc tcccctcctg ccacaactgg tggtcgcggt ccactttgtg
     3061 ccgggccttg tgccaggcga gctgtttcaa gagagtccgt gcccgtcgct cctcaaggaa
     3121 gtcgatgatc tgccgagcgg tgaacgactt gaagtcaccc atgtcctccg ccagattcag
     3181 cgacgatgcg atcaggtgaa ggtggttctc cagaatcacg taaccgtaga tttcaaatcc
     3241 gcgattcgcc cgcagccagg tccacgaatc gaagacgatc tgaaccgctt cggggcgggt
     3301 gaacaccggc agccagccta cgatcgtgca ggtcatgaag tgcgggcagg cgttgtcgca
     3361 gatgcggtag cggctgcggg tcatggcagg agtatagcga atggcaggac gcggagcgtc
     3421 ggagcgtgcg ttcccacgca gagcgtggga acgagcgggt gtgcgtgcct cggtcgagcc
     3481 gctacagctt cgctacgaaa tggacctgcc gggcgacctc gatgaagccg aggtcgcggt
     3541 aaagatggtt gccgatggcg ttctgggcga gcgtttcgat cttggcgtgc gtcaggccgc
     3601 tggcgcggaa gtgggccagg gcgtgctcga tcaaaatgcg gccgagcccc tggccgcggt
     3661 attccggacg gatcgcgata ttcgggatgt ggccgatccc tgcctcgcgg tcctgccagg
     3721 tggtgatgta gccgacgata gtttcattct gccctttcac ccccggcccc tctcccagga
     3781 ggggagaggg gagcggaacg ccacggaggg cgttccctac agtactctcg accgccacaa
     3841 aaatccccgc tgggt
//
LOCUS       DASUGB010000764         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_362451, whole genome shotgun sequence.
ACCESSION   DASUGB010000764 DASUGB010000000
VERSION     DASUGB010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362451"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="dapF"
                     /locus_tag="VFV87_14685"
     CDS             complement(<1..872)
                     /gene="dapF"
                     /locus_tag="VFV87_14685"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5105062.1"
                     /translation="MSIPYYKAQAVGNDFLFSWISELPEELRKCAEAEFLAGARVKKD
                     EDSPDELARAARAVCHRHLGVGADGWYLMHETPNADLSIHLFNSDGSRAELSGNGTRC
                     AAGVLLWENRPLAEPDRVRVETGSGVKQLRLMSRKDNEFLLEMEMGKPQVAPDEVLTS
                     LPLASGSRDVTILNVGNPQCALAVKDLKFDWRAVGAEIEGHPYFPKRTNVSFFRKVGP
                     KAIEARFYERGAGPTLSSGTGSTGAAAAAILLRMVAPEVEVITEAGSMQLRWENPPEG
                     SIFVDGPAEVIARG"
     gene            complement(869..>2185)
                     /locus_tag="VFV87_14690"
     CDS             complement(869..>2185)
                     /locus_tag="VFV87_14690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5105063.1"
                     /translation="SLAVLFLFLLARRLTGSAAAGVAAVGVFALNPNLLYLQSTAMTE
                     APYFACGLAALYCLARAADESGWLWPALAGVAALCGTLTRYDGWFVLPFYALYLLLAS
                     GKPRWGATALFLAIAGIGPLVWMLHNWWWWGDPLEFYRGEWSSRAIYDRALRGGMKPA
                     PGDGDWRLAFLYYTTAVRHVCGGVLAVCGGLALATCARRRLAAPAGLLLLAPVFYVWS
                     LHSSGLPIFVPDLWPNTYYNTRYALSILPVFALAMAGLVSLTSGGKLRGLALALSLAV
                     AALPWLFYPRAAAWICWKESQVNSIARRHWTNDLAEFLKENYRGGGIAMSFGDARGAL
                     RQAGIPLREGMHEGSGVRWYGALARPEFFLQEEWAITLSGDKLSNALPRGERHGLRYD
                     CQQTIEVTGGPVVKIWRRSSRIRPPEFAPSEDEQRIHFEEALREVP"
BASE COUNT          431 a          773 c          594 g          387 t
ORIGIN      
        1 tcaccgcgtg cgattacctc ggccggacca tcgacaaata tcgagccctc cggtgggttc
       61 tcccatcgca actgcatgct gcccgcttcg gtgatcactt ccacctcggg cgccaccatc
      121 cgcaacagaa tcgccgccgc cgccgctccc gtggacccgg tgcccgagct gagagtaggc
      181 ccggctccgc gctcataaaa tcgtgcttcg atcgccttcg gccccacctt ccggaagaaa
      241 ctgacattgg tccgcttcgg aaagtacgga tggccctcga tttcagcgcc caccgctcgc
      301 cagtcgaact tcagatcctt taccgcaagg gcacattgtg ggttgccgac gttgaggatt
      361 gtgacatccc tcgatccgga ggctaatggt agcgatgtaa gtacttcatc cggcgctact
      421 tgcggtttcc ccatctccat ctccagcaag aactcgttgt cctttcgcga catcagccgc
      481 aactgtttca ctccggaacc cgtttccacc cgaactcgat ccggttcagc cagcgggcga
      541 ttttcccaca gcaacacgcc ggctgcacat cgcgtaccat ttccggacaa ctcggcccgg
      601 ctgccgtcgc tgttgaacaa gtgaatcgac aggtcggcgt tcggcgtttc gtgcatcaga
      661 taccagccgt cggctccgac tcccagatga cggtgacaga cggctcgcgc agccctagct
      721 aattcatcgg gagaatcctc gtcttttttc acccttgctc ccgcgagaaa ctccgcctcg
      781 gcgcattttc tcaactcctc cggcagttct gaaatccagc tgaacaggaa atcgttaccg
      841 actgcctggg ctttgtaata cggaatgctc atggaacctc gcgcaatgcc tcctcaaagt
      901 gaatccgctg ctcgtcctcg ctgggtgcga actccggcgg gcggattcgt gaacttcttc
      961 gccatatttt caccaccgga ccgcctgtga cctctattgt ctgctgacag tcataccgca
     1021 gtccatgccg ttcgccccgc ggcagagcgt ttgacaactt gtcgccggag agcgtgattg
     1081 cccattcttc ctgcaggaag aactctggcc gtgccagcgc gccgtaccac ctgacgccag
     1141 acccctcgtg cattccctcg cgcagcggga tgcctgcttg ccgcagcgcg ccgcgcgcgt
     1201 cgccgaacga catcgcaatg cctccgccgc gatagttctc cttcagaaac tcggccagat
     1261 cgttcgtcca atgccggcga gcaatcgaat tcacctgcga ctccttccaa cagatccagg
     1321 ccgccgcacg tggatagaag agccacggca gtgccgccac cgcaagcgac aacgccagcg
     1381 ccagcccgcg cagtttcccg ccggaggtga ggctcaccag cccagccatt gcgagggcga
     1441 ataccggcag tatggacagc gcgtagcgcg tgttgtagta ggtgtttggc caaaggtcgg
     1501 gcacgaagat cggtagtccc gaactgtgca gactccacac gtaaaacacc ggcgccagca
     1561 gcagcaaccc ggccggagct gccagccgcc gtcgcgcaca tgtcgccagg gccagtccgc
     1621 cgcataccgc caaaactccg ccgcagacgt gccgtacggc cgtcgtgtag tagaggaacg
     1681 ccagcctcca atcgccgtcc ccaggcgccg gcttcattcc tccgcgcagt gcccgatcgt
     1741 agatagcccg cgacgaccac tcgccacggt agaactccag cggatcgccc caccaccacc
     1801 agttgtgcag catccacaca agcggtccaa ttccggcaat cgccaaaaac agggcggtgg
     1861 cgccccagcg cggctttccc gatgccagca acaaatagag ggcatagaac gggagcacga
     1921 accagccgtc atatcgcgtg agcgtgccgc acagcgcggc cactccagcc aacgccggcc
     1981 acagccagcc gctctcgtcg gcggctcgcg ccaggcaata gagcgccgcc agtccgcagg
     2041 cgaaatacgg cgcctcggtc atggccgtgg actgcaagta gagtaggttc gggttgagcg
     2101 cgaagacgcc cactgccgcc acgcccgccg ccgcgctgcc cgttagccgc cgcgccagca
     2161 ggaagagaaa caggaccgcc agcga
//
LOCUS       DASUGB010000765         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1196048, whole genome shotgun sequence.
ACCESSION   DASUGB010000765 DASUGB010000000
VERSION     DASUGB010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196048"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFV87_14695"
     CDS             <1..414
                     /locus_tag="VFV87_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105064.1"
                     /translation="LLWRGRSYLYEDHVDAALADADRACRLDPQDHEARILLAICFCA
                     AGQFGESLVEFRRVRALGPDDIAAWFYRGIAHAERGQMHEAVADFSHVIELDPHDSEA
                     WYERGRCHEDLGNRTAAKADFAKARELGWLDGKTE"
     gene            551..1300
                     /locus_tag="VFV87_14700"
     CDS             551..1300
                     /locus_tag="VFV87_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105065.1"
                     /translation="MSLSRREFARQSLGGLLTYSLLQTLFSRDAFGDEMKLVAAKWLK
                     DLNDLSQEAKSGKLKQTDWQTKVDELFDKIDLAEALQFIDFEKLTANLKFRDQGELSL
                     RAKLPQVEGLPTDLVFGHQVFALTKDRSVVPHGHNNMATAFLILKGTFRGRHYDRLED
                     DGEYMIIRPTIDRAFGPTEHSTVSDYKDNVHWFTATSDQAFIFNIHVMNVDPAKKNTG
                     RVYIDPSGEKLSDNRIRARRVSAGEVYKLYG"
     gene            1352..2161
                     /locus_tag="VFV87_14705"
     CDS             1352..2161
                     /locus_tag="VFV87_14705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5105066.1"
                     /translation="MDFYLNGQVAAVIGGASGIGLAIAKEFAREGCRVAVIDKSAQTD
                     RIAGDVAAAGRTTLIGIVADVTDYAAMQDAAARAAAALDKVQHVVYAAGCGSGKFGFP
                     FWNLEPSDWERVLRVNLLGAVHTAHAFAPSFVEQKRGTMLFISSVAGQIGSQTDPPYS
                     SAKAGLINFAQVAAKDFAPHNVRVNTICPGMVQTPLNKAVWQSWNDRQPAESKLTYDQ
                     WAGAKVKQMCPLGRWQTPEDIAALTVFLASDHASNITGQTMNVDGGWVMHW"
     gene            complement(2198..3256)
                     /locus_tag="VFV87_14710"
     CDS             complement(2198..3256)
                     /locus_tag="VFV87_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105067.1"
                     /translation="MRTAILASLGVIVLIAPAQRNCISEDQFPVPVAALSDRFALIGK
                     LHVPLGKLVTVQGVVVEGPFKGYEGGPNLRVQRIGAKYTQEDIQIPLSDMTQVVPEKR
                     VEIKIGETYEFEGYETGGYVGIPSEVWKGGPAWVQTTNHYFRLDFNYVRGKQIDPIRF
                     QPYDFQGRRALFEGVARNREGVAIMDGEKWTVIVDATMPWPERLVGKHVETLGLHNPT
                     KESKVFLLVDGTWRPVSLDDQIGQRVELRGTARSFNDIWWFRFRDTDMYVEDMDQLPG
                     WSNDCWGKPIVIRGVLDKSKLPRLDQISLRSDRGLRDHYIVRKAQWEPLSTLLSPERS
                     SGEPGVADLTKLRNDDER"
     gene            complement(3310..3678)
                     /locus_tag="VFV87_14715"
     CDS             complement(3310..3678)
                     /locus_tag="VFV87_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105068.1"
                     /translation="MAARANDRHGFGNDLVRLRRSDTGSRCVISGALQRRRNSRVGRR
                     LRQRRIIAFGHDGNHDLLVRLQRAANDVFQSGPIRPGKIIDITADSLHASSLRRTASK
                     ANQIRLPMHHPAAALRIRIQ"
     gene            3872..4156
                     /locus_tag="VFV87_14720"
     CDS             3872..4156
                     /locus_tag="VFV87_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105069.1"
                     /translation="MRPIHGLSDSKIDQFVDDLVLIADLVDPPVASGISTDPDDDAVI
                     AAAKLGRAAVLCTLDRHLHEPNARAYCAALGTEVLTDIDLLSRLRGAGKS"
     gene            4259..>4783
                     /locus_tag="VFV87_14725"
     CDS             4259..>4783
                     /locus_tag="VFV87_14725"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5105070.1"
                     /translation="MGLENRDYYRESDYTRALLGCGLEYFSPVVKWLIIANVAVFLAQ
                     IFITRPMTLDDWKAQIEEYPREVRESMLKAMEAAQKQREQEKNGDSGGEFTIDEPLPE
                     EWEYEEMLFGGQQISTVQEWLELDSRKVLRGQVWRVLTYAFCHERSGVWHILFNMLGL
                     YWFGVTLESMYGQRE"
BASE COUNT          934 a         1465 c         1419 g          965 t
ORIGIN      
        1 ctcctgtggc gggggcggtc ctatttgtat gaagaccacg tcgatgcagc cctggccgat
       61 gccgatcgcg cttgccgttt ggacccccag gatcacgaag cgcgcatctt gctggcgatt
      121 tgcttttgtg cggcgggaca attcggggag tcgctcgttg agtttcgccg cgtccgggca
      181 ctcgggcccg acgacatcgc tgcctggttc taccgcggta tcgcccatgc cgagcgcggt
      241 caaatgcacg aggccgttgc cgatttttca cacgtcattg agctcgatcc tcacgatagc
      301 gaggcctggt acgagcgcgg ccgttgccac gaggacttgg gaaataggac cgctgccaaa
      361 gcggatttcg ccaaggcgcg ggaactgggg tggctcgacg gaaagacgga gtgaaggaga
      421 gagagcgaag gagagcggtc ctcgctcctt ctcaccgtct cccattctct cctgctctct
      481 gtctagaaat cgccgcgccc cgcctctatg atgttggcag gttgcatccc gccactcttg
      541 gagtcccgcc atgtcccttt ctcgccgcga gtttgcccgg caatcgctgg gcggtttgct
      601 cacgtattcg ctcttgcaga cgctcttttc gcgcgacgcc ttcggcgacg agatgaaact
      661 ggtcgccgcg aagtggctga aggacttgaa cgacctgagt caggaagcaa agagcggcaa
      721 gctcaagcaa accgactggc agacgaaggt ggatgagctg ttcgacaaga tcgacctggc
      781 agaggcgctt cagttcatcg actttgagaa gctcaccgcg aacctcaagt tccgcgacca
      841 gggagagctc agcctgcggg cgaagctgcc gcaagtcgag ggcctgccga ccgatctggt
      901 cttcggccat caggtctttg ccctcacgaa ggaccgctcg gtcgtgccgc atggtcacaa
      961 caacatggcc acagcgtttt tgatcctcaa aggcacgttt cgcggccggc actacgaccg
     1021 cctggaggac gacggcgagt acatgatcat ccgcccgacg atcgaccggg cctttggacc
     1081 cacagagcac tcgaccgtgt ccgactacaa ggacaacgtc cactggttca cagcgaccag
     1141 cgaccaggca ttcatcttca acatccacgt gatgaacgtc gatcccgcca agaagaacac
     1201 cggccgcgtc tatatcgatc cgagcggcga gaagctcagt gacaaccgca tccgcgcccg
     1261 ccgcgtcagc gcgggcgagg tgtataagct ctacggctaa tcacacaagc ccgaagcgca
     1321 agcgaggggc gctgcgtgag ggacgcgtcc catggatttc tacctcaacg gtcaggtcgc
     1381 cgcggtgatt ggcggcgcga gcggcattgg gctggcgatc gccaaggaat ttgctcgcga
     1441 gggctgccgc gtggcggtga tcgacaagag cgcgcagacc gaccggatcg cgggcgatgt
     1501 cgcggcagcg ggacgaacaa ccttgatcgg catcgtcgcc gatgtgaccg actatgccgc
     1561 gatgcaggat gccgcggccc gtgcagccgc tgcactggac aaggtccagc acgtcgtcta
     1621 tgccgccggc tgcgggtcgg gcaagttcgg ctttcccttc tggaacctgg agccgagcga
     1681 ctgggagcgc gtgctgcggg tgaacctgct cggcgcggtc cacacagcgc atgccttcgc
     1741 gccgtcgttc gtggaacaaa agcgcgggac aatgctcttc atctcgtcgg ttgccggcca
     1801 gatcggctcg cagaccgatc cgccctacag cagcgccaag gcgggcctga tcaacttcgc
     1861 ccaggtcgcg gccaaggact tcgccccaca caacgtccgc gtcaacacga tctgccccgg
     1921 catggtccag acaccgctga ataaggcggt ctggcaatcc tggaacgacc gccagccggc
     1981 tgagtcaaaa ctaacctacg accaatgggc cggcgcgaag gtcaagcaga tgtgtcccct
     2041 cggccgctgg caaacccccg aagacattgc cgccctcacc gtctttcttg cctcggacca
     2101 cgcctccaat atcaccggcc agacgatgaa cgtcgatggc ggctgggtga tgcactggta
     2161 gcagactcgg ctgacgccgc agcatttgcc aaacggctca gcgctcgtcg tcgttcctca
     2221 gtttcgtgag atcggcgacc cccggctctc ctgatgatcg ctcgggcgag agcagtgtgc
     2281 tcagaggttc ccactgtgcc tttcgaacaa tgtaatggtc gcggagaccg cggtccgacc
     2341 gtagcgagat ttggtcgagc cgaggaagct ttgacttgtc caagacgcca cgaattacga
     2401 ttggtttgcc ccagcaatca ttcgaccagc ctggcaattg atccatgtct tcgacataca
     2461 tgtctgtgtc acgaaagcga aaccaccaaa tatcattgaa acttctagcc gttccacgca
     2521 actctacgcg ttgtccaatc tgatcgtcca aactcaccgg acgccaggtg ccatccacga
     2581 gcagaaatac cttcgactcc ttcgtgggat tgtgcagtcc gagcgtttcg acatgctttc
     2641 cgacaagtcg ttccggccac ggcattgtcg catcaacgat gacggtccac ttctcgccgt
     2701 ccatgatagc gactccctct cggtttcgag cgacgccttc aaagagtgcc cttcgacctt
     2761 ggaaatcgta gggctgaaat cggataggat cgatttgctt tccgcggacg tagttgaagt
     2821 ccagtcggaa ataatggttg gtcgtctgaa cccaagccgg ccctcctttc cagacctcag
     2881 atggaatgcc gacataccct cctgtttcgt aaccttcaaa ctcataggtc tcgccgatct
     2941 tgatctccac tcgcttttct ggcacgactt gggtcatgtc gctgagcggg atctgtatgt
     3001 cttcctgcgt gtacttcgcg ccgatccgtt gcactcggag gttgggacca ccctcgtagc
     3061 ccttaaaagg accctcaaca acgacgcctt gaacggttac aagtttcccc aacggtacat
     3121 gcagtttgcc gatcagggca aaccgatcgc tcaaagccgc cacgggcact gggaattggt
     3181 cctcagaaat acaattcctc tgtgccgggg caatcaacac aattactccg aggctcgcca
     3241 agattgcagt gcgcatcggg cgctcctaca ggtgggcaag gatggactca ccgggaattc
     3301 taatgcgcac tattgaattc tgatacgtaa ggcggcggct gggtgatgca ttggtagccg
     3361 gatctgattg gctttggatg cggtgcggcg taaactagaa gcatggagac tgtcagccgt
     3421 aatgtcgatg atcttgccgg gccggatcgg gccgctttgg aacacgtcat tggccgccct
     3481 ttgcagtcgg accagcagat cgtgattgcc gtcgtgccca aaggcgatga tgcggcgctg
     3541 acgcaggcgg cggcccacgc gcgaattgcg gcgacgctgc agcgcgccgc tgatcacgca
     3601 acggctgccg gtgtcactcc gtcgcaggcg gaccaaatcg ttgccgaagc catggcggtc
     3661 gttcgcccgc gcagccattc tgacggcggg ccccgcagcg gttcgccatg aggctgctcc
     3721 tcgatacaaa catcctggca cggtctgcgg ttgggccacc tggcccggcc catgctcttt
     3781 tgttgctggc gacgcatcat gatcacacct tgattctgtc gccttttctc atcgcctcgc
     3841 cgagcttgct cgcgttctgc gttacgatcg cttgcgaccc atccatggtt tgtcagatag
     3901 caagatcgat caattcgtcg atgatttggt cctgatcgcc gacttggtcg atccgccggt
     3961 tgcatccggc atcagcaccg acccggatga cgacgcggtc atcgccgcgg cgaagctggg
     4021 gcgcgccgca gtgctttgca cgctggaccg ccatcttcac gagccaaatg cacgggcgta
     4081 ttgcgccgcc ctcgggacag aagtgctcac cgatattgac ttgctgtcgc gcctgcgtgg
     4141 ggccggcaaa tcctagcctc tctgagccga tgcctacatg agcttgagac ctgtcgggcg
     4201 gggtgcgaca atctgctgac tcctcatctc ccgtggcatt cgacccagcg gtgcaaacat
     4261 gggcctggaa aaccgcgatt actaccgcga atcggattac acgcgcgcgc tgttgggctg
     4321 tggcctcgaa tatttttcgc ccgtcgtcaa gtggctgatc atcgccaacg tggcggtttt
     4381 cctggcacag atcttcatca cgcgaccgat gacgctggac gactggaagg cccagatcga
     4441 ggaatatccc cgagaagtcc gcgagtcgat gctcaaggcc atggaagcgg cccagaagca
     4501 gcgggagcag gagaagaatg gcgattcggg tggcgagttc acgatcgacg agccgctgcc
     4561 ggaggaatgg gagtacgaag aaatgctgtt tggcgggcag cagatttcca cggtgcagga
     4621 gtggctcgag ctcgattcgc ggaaagtcct ccggggccag gtgtggcgcg ttttgaccta
     4681 cgccttttgc cacgagcgct ccggtgtctg gcacatcctc ttcaacatgc tcgggctgta
     4741 ctggtttggc gtgacgctcg aatcgatgta cggccagcgt gaa
//
LOCUS       DASUGB010000766         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_639609, whole genome shotgun sequence.
ACCESSION   DASUGB010000766 DASUGB010000000
VERSION     DASUGB010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639609"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2778
                     /locus_tag="VFV87_14730"
     CDS             <1..>2778
                     /locus_tag="VFV87_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5105071.1"
                     /translation="QRRDLLRTTLASIGDAVITTDVQGQATFLNPVAESLTGWLQAEA
                     YGRPLEALFQIVHDRTRESVENPAWRALREGAVIGPAKDALLVAKDGAERLIESSASP
                     IRNERGELAGCVLVFRDVTERRRSEQALERKERELTDFFDNANVGLHWVGPDGTILRA
                     NQAELDLFGYRREEYVGHKITEFHLDRPVIDGMLVRLLRGETLHDFAARLRCKDGSIK
                     YVLINSTALIEDGKLIHTRSVTRDITSRKRAAEARALLAAIVETSDDAIISKSLDGVI
                     LSWNAGAERLFGYTAAEAIGQSITFIIPPERQQEEPMILERLRAGRRIEQFETVRVAK
                     EGRRIDVALTISPVLDRLGRVVAASKVARDITARKRAEEALRESEERFARFMQHLPGL
                     AWIKDAEGRYVYVNHSAEKAFQKPAAELYGKTDEEVFPPATAAAFRENDRRALATKTG
                     MQTTETLEHEDGLVRHSIVSKFPILDADGSVVLVGGMAIDVTERIRAENALRESEARF
                     RLLADAAPVMIWISGIDKLCTWFNRPWLDFVGRPLEQELGDGWMAHVHPLDLQRCLQC
                     YAAAFEAREPFSLEYRLRRHDGHYRWVLDTGIPLRDAAGEFTGFIGSCLDITDRKQAE
                     VTLRESEARKTAMVQAAIDCIITMNHHGRIVEFNPAAERTFGYSREQALGREMVELLI
                     PKALRERHRRGLARFLATGEGPILDQRIEMPALRADGTEFPAELSVTRIAAEGPPVFT
                     AFLRDISERKRAEESLKESEQRLRLALDAGRMGTWEWDIASNSVAWSPGLEAIHGLAA
                     GSFPGTFEAYQQDIHPEDKEQVLGSIQRTLQEGKDYHIEYRIVWPDGSLRWVEGRGRL
                     VRDDQGQPLRMVGVCTDITSRKHAGDALKAKEAELELVAKTTPVMLTRCSRDLRFLFV
                     NHATAALFGGA"
BASE COUNT          566 a          819 c          904 g          489 t
ORIGIN      
        1 gtcagcgacg cgacttactt cgcacaacgc tcgccagcat tggcgacgcg gtcattacga
       61 ccgatgtgca aggccaggct acctttctca acccggtcgc agaatcgctg acgggctggc
      121 tgcaagctga agcttacggc cggccgttgg aagcgctttt tcagatcgtt cacgatcgga
      181 cgcgggagag cgtcgagaat cctgcctggc gggcgctgcg agagggggcc gtgattggac
      241 ctgcgaaaga tgcgctgctg gttgccaaag atggcgcgga gcggcttatc gagagcagcg
      301 cttctccgat tcgcaatgag cgcggggaat tggctggctg cgtcctcgtg ttccgcgacg
      361 tgaccgagcg caggcgcagc gagcaggccc tggagcggaa ggagcgcgaa ctaaccgact
      421 ttttcgacaa cgccaacgtg gggctgcact gggtcggccc ggacggcacg attctgcggg
      481 ccaatcaggc ggaactggat ctcttcggat atcgccgcga agaatacgtg ggccacaaaa
      541 tcactgagtt ccacttggat cggcccgtca tcgacggcat gctggtgcgg ctcctgcgcg
      601 gagaaacgct acacgacttc gcggcccgtc tgcgctgcaa ggacggctcg atcaagtatg
      661 tcctgatcaa ctccacggcg ctcatcgaag atggcaagct catccatacc cgcagcgtca
      721 cccgcgacat caccagccgc aagcgagctg ccgaggcgcg ggcgctgctc gccgcgatcg
      781 ttgaaacttc ggacgacgca attatcagca aatcgctgga cggggtcatc ctctcgtgga
      841 atgccggagc cgagcgcctg ttcggctata ccgcggcgga agcaatcgga cagtcgatca
      901 ccttcattat tccgcccgag cgacagcaag aagagccgat gattctcgag cggctgcgag
      961 ccgggcggcg cattgagcag ttcgagaccg tgcgagtggc aaaggagggc aggcggattg
     1021 acgtcgcctt gacgatctcg cccgtcctcg accgactggg ccgcgtggtt gccgcctcca
     1081 aagttgcccg cgacattacc gcccgcaaac gggccgagga ggcgctgcgc gagagcgaag
     1141 agcgatttgc caggttcatg cagcatctgc cgggcctggc atggatcaaa gacgccgagg
     1201 ggcgctatgt ctatgtgaat cactcggcgg agaaggcctt tcaaaagccg gctgccgaac
     1261 tttatggcaa gacggacgag gaggtatttc ctccggcaac ggcagccgcc ttccgcgaaa
     1321 acgatcgccg cgcgctggcg accaaaacgg gaatgcagac aacggagacg ctcgagcatg
     1381 aggacggact cgttcgtcat tcgatcgtca gcaaattccc gatactcgac gccgatggca
     1441 gcgtggtgct cgtgggcggg atggccatcg acgttaccga acggattcgg gccgaaaacg
     1501 cccttcgcga aagcgaagcg cgattccgcc tgctggccga cgccgccccg gtcatgatct
     1561 ggatcagcgg catcgacaag ctttgcacgt ggttcaatcg cccgtggctg gatttcgtgg
     1621 gacgcccgct ggaacaggag ctgggcgacg gttggatggc gcacgtgcat ccgctggatc
     1681 tgcagcggtg cctgcagtgc tatgcggcgg cgttcgaagc ccgcgagccg ttctcactcg
     1741 agtaccgcct gcggcgtcac gacggtcact accgctgggt tctggatacc ggaattccgc
     1801 tacgcgacgc ggccggcgaa ttcaccggtt tcatcggctc gtgtttggac attaccgacc
     1861 gcaaacaagc cgaggtgacg ctgcgtgaaa gcgaagcccg caaaacggcg atggtgcagg
     1921 cggcgatcga ttgcatcatc accatgaacc atcacggcag gatcgtggaa ttcaatcccg
     1981 ctgccgaacg gacgtttggc tattcccgcg agcaagcgct gggacgtgag atggtcgaac
     2041 tcttgattcc caaggcgctg cgcgagcggc atcgtcgcgg gctggcccgt ttccttgcaa
     2101 ccggcgaagg accgatcctt gatcaacgca tcgaaatgcc cgccttgcgg gccgatggaa
     2161 cggagttccc ggcggaactg tcagtcactc gcattgccgc ggaaggaccg ccggtattca
     2221 ccgcatttct ccgagacatc tccgagcgca agcgggccga agaatcgctg aaagaaagcg
     2281 agcagcgctt gcggttggct ctggatgcag gccgaatggg cacctgggag tgggatatcg
     2341 cctcgaattc agtagcgtgg tcaccgggat tggaagcgat ccacggcctg gcagcaggaa
     2401 gttttcctgg aacgtttgaa gcctatcagc aggacataca ccccgaggac aaagaacagg
     2461 tgctggggag cattcagcgg accctgcaag agggaaaaga ttaccacatc gaatatcgca
     2521 ttgtctggcc cgacggaagt ctgcgctggg tggaaggccg cggcaggctg gttcgggatg
     2581 accagggcca gccgctgcgg atggttgggg tgtgcaccga tattaccagt cgtaaacacg
     2641 ccggcgacgc tctgaaagcg aaagaggccg agttggagct agtcgccaag acgacgcccg
     2701 tgatgctcac gcgttgcagc cgcgacttgc gcttcctgtt cgttaaccac gccacggcgg
     2761 ccctgtttgg tggagcgc
//
LOCUS       DASUGB010000767         5759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1057552, whole genome shotgun sequence.
ACCESSION   DASUGB010000767 DASUGB010000000
VERSION     DASUGB010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057552"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="VFV87_14735"
     CDS             <1..1778
                     /locus_tag="VFV87_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5105072.1"
                     /translation="NILLGLSGGGFRATLFHLGVLRAFRCHRLLGSIRAISSVSGGSI
                     TSAHLVSNWHEYSNYDVDHFDRLETELFKFVSRGIRERIILRWPAALCIRRTSTDLLL
                     GEYRRLFGTDKKLAGLHSDKNVPRLFLMTTNLSRHTAHSYFSHNGYTLDRATIPIVTM
                     GLAEAVTASSAFPMIFPAFVLDYAKVGVSTSGAGVTRDYLTDGGVFENLGLTVLREHA
                     SSLQLVPPDQATIVLSNAGKTIDWDLETNFARRLFGNLNRAIDVMQYWAEKRFLSSLS
                     EGEHEISIAESIPPHPGSANMPPEIVQKAISYLRTDLDMFSMLHCRMLYDHGFTLASS
                     LIDKMGWSTVEKHANPYQPWSDDTLRQCQHPRTIHLLRAPVRIFPRLQALWDVAIVPY
                     VLAILICVCGIWALTPRISQAISAAIRWIDEQQFESHTSEDIRFNRPMVSTTLRDRLD
                     FVTTGMSREAKAAIDACRRRQISEFPGNVVVIVEQETPFSADYQSMHRWLRFSGSGGS
                     GNVRAMHGAAFVLDTNGNHEGPRYREVRMAQKVNGELQGCVDVFQPRENESIVMVVVA
                     AAIIEKEPITVMDVEQSTFTLGVSQ"
     gene            3189..4664
                     /locus_tag="VFV87_14740"
     CDS             3189..4664
                     /locus_tag="VFV87_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105073.1"
                     /translation="MHPHFAKTQFETRRQFLKQCNTGLGALALAALAGNSAPAAPLPD
                     VDNPLSPKAPPLPAKVKRVIYLHMSGAPPQHDLWDYKPKLVEHNLEPCPDDLLAILQK
                     ERLPFIDLKARRPKMLGTPYKFEKRGQSGLEMSDIWKHLPEVADDLCVVKSMTTDQFN
                     HAPAELFLYTGSPRNGGAAMGSWITYGLGSENQDLPGFVVLISGGTDPTGGKALWSTG
                     FLPSVFQGVQCRTVGEPILYANNPDGLSREVRRRSLDALKRLNELELQEFGDPETLTR
                     ISQYELAYRMQIAVPDVMDISKESKEVLAAYGAEPGKASFANNCLLARRLAEQGVRYV
                     QLYDWGWDMHGTGADNDIVTGLPKKVKDVDQPITALMKDLKQRGMLSDTLVVWGGEFG
                     RTALNEERGGSKFLGRDHHPHCFSIWLAGGGIKPGHSYGATDDLGYFVTENKVGIHDL
                     QATILHLLGFDPQRFSFPYQGLNQRLIGPSDEPRVVQEILA"
     gene            complement(4689..5141)
                     /locus_tag="VFV87_14745"
     CDS             complement(4689..5141)
                     /locus_tag="VFV87_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5105074.1"
                     /translation="MNTSKILFPTDFSTAGQAALEMATSLARDRGATLVIVHVEEPPM
                     AYGGGELYYGVQEPDREELKRMLSDVVPTDPQVPCEHKLMIGSPATAIVDLAEKENAD
                     LIVMPTHGRTGLMRVLMGSVAEEVVRKAGCPVLTVKATAEKPIKESTK"
     gene            5433..>5759
                     /locus_tag="VFV87_14750"
     CDS             5433..>5759
                     /locus_tag="VFV87_14750"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5105075.1"
                     /translation="MHAVGALWLALIPLAATDGALDSPVGRKIQNFELHDHLGTPHAL
                     KDWADKRAVVVVFLGTECPLAKQYGGRLAELAERYQSKEVQIIGINSNQQDSLAEISH
                     FVREQKI"
BASE COUNT         1164 a         1696 c         1737 g         1162 t
ORIGIN      
        1 tgaacatctt gcttggacta tccgggggtg ggtttcgagc gacactgttc caccttgggg
       61 tactcagggc atttaggtgt caccgacttc ttggaagcat tcgggcgatt tcctcagtct
      121 cgggaggctc gatcacttca gcccatctcg tcagcaactg gcacgagtat tccaactacg
      181 atgtcgatca ttttgatagg cttgaaaccg aactgttcaa gtttgtaagc cgagggatcc
      241 gcgagcgaat aatccttcgt tggcctgctg cactctgcat taggcgcact tccactgact
      301 tgctccttgg agagtatcgt cgcctatttg ggacagacaa aaagctcgcg ggactgcact
      361 ctgataaaaa tgtgccgcga ctattcctca tgacgacgaa tttgtcgcgc cacaccgcac
      421 attcgtactt ctctcacaac ggttatacgc tagaccgcgc cacaattcca atcgttacga
      481 tgggacttgc tgaggccgtt accgcgtcat cagccttccc tatgattttt ccagcattcg
      541 tccttgacta tgccaaggta ggagtgtcaa cgtcaggagc tggcgtaacg cgtgactatc
      601 tgacagatgg cggtgtattc gagaatctgg gactcacggt cttgcgcgaa cacgccagca
      661 gtttgcagct agtgccacct gaccaagcga cgattgtgtt gagcaatgca ggaaaaacca
      721 tcgattggga tttggaaacg aatttcgcgc gccgactttt tggcaacctc aaccgcgcca
      781 ttgacgttat gcagtattgg gccgaaaagc gtttcctctc atcactttct gaaggtgaac
      841 atgagatttc tatagccgag tccattccac cgcatcccgg aagcgccaac atgccgccag
      901 agatcgttca gaaggcaata tcttaccttc gaactgattt ggatatgttc tctatgcttc
      961 actgcaggat gctctacgac catggtttta cgctcgccag tagccttata gacaagatgg
     1021 gatggtcaac cgttgaaaaa cacgcgaatc cctatcagcc atggtcggac gacactctgc
     1081 gccagtgtca acacccgaga acaattcact tgctccgtgc gcccgttcga atcttcccac
     1141 gattgcaagc gctctgggac gttgccatcg tcccgtatgt acttgcgatt ctaatctgtg
     1201 tctgcggaat atgggctctg actccgagaa tatcgcaagc gatatcggcg gccatcaggt
     1261 ggatcgacga gcagcagttt gagagccata cgagtgagga tatcaggttt aatagaccaa
     1321 tggtaagtac cacgctacga gacaggctag acttcgtaac gactggaatg tcgagagagg
     1381 ccaaagcagc gattgacgcg tgtcggcggc ggcagatttc tgagtttcct ggaaacgtgg
     1441 ttgtcattgt tgagcaggag acaccgtttt cggcggatta tcagagtatg catcgatggc
     1501 taaggttttc tggttccggc ggatctggga acgtgcgggc aatgcacggg gctgcgttcg
     1561 tgctcgacac gaatgggaat catgaaggcc cacgatatcg tgaggtaagg atggcgcaga
     1621 aagtgaacgg ggaactccaa ggatgcgtcg atgtgttcca acctcgagag aacgagtcaa
     1681 tagtgatggt cgtggtcgcg gccgcaatta tagagaagga gccaataacg gtcatggacg
     1741 ttgagcaaag taccttcacc ttgggagtct cacaatgaga gtagttgctg tgtttgtcac
     1801 gatgtggttg gtcttgatcg tttccgcggt gcaggcaggt agcattgagg gtattgtgcg
     1861 agatctagat ggcacgcctc tcggtggtat cacggtagag gcatttgaag taaatgcgca
     1921 gggagttcgt aatcgaacgc cgttcaattc gacggtcagt aacagcacgg ccgatccagt
     1981 gcgcggaccc gtcgggcatt acactctcga tttgatgaat cggccgcgtg tcgtcgttct
     2041 ctattcaggt cagggtcgaa atagcgtatt cgtcccgcaa ccgcagcaac caaacattgc
     2101 cggcgttgga ggtgcgatcg caggattcaa tgacatacgc ggatttgacg tattggtgcc
     2161 ggaagtcagg ccgattgttt gtccgtgcgc ccgcgggcat tggcgtcgat gtcgacggtg
     2221 ttggtgaggc gtgtggattg ccggagcgga accacggcct cgcggcgcga aacaaccctg
     2281 gccccgctcg gcgctggccg ccaagtggtt cggccaggcc cttggccgaa cggaaccgcc
     2341 gcgcggcgtg cacatcgtag cccgtagcgc aagcgagggt aatggacgtt gctccctgcc
     2401 cgacgtgacg ccgcgcaagt gcccgcccgc gcgcctccct ccccgccttg ccgctcattc
     2461 gacgtgccac ccacaatacc ctcgcttgcg ctacgggcta cgatgccgga agccgctctc
     2521 gccgaaggcg aaccacggcc tcgcggcgcg aaacggctct ggccccgctc ggcgctggcc
     2581 gcataggggt ttggccaggc gcttggccga acggaaccgc cgcgcgaccc gccaatctta
     2641 gcccgcagcg caagcgaggg taatggacgt tgattcccgc ccggtgtggc gtcgcggaat
     2701 atgcctgatc gcgcccgcgc actccctcgc acatccgtga tcgtccgccg cgccacccgc
     2761 aattccctcg ctggcgctac gggctacgat gcggaggtga accacggccg cgcggcgcga
     2821 aacgaccctg ggccagattg agttacgcct tcggcgtatc agggtttggg tttacctggc
     2881 ctggggatcg tgccatgatg gagaatgcac aacagtctga gacgagcctg aaatgcctgg
     2941 gcaagttcct cgacgggcta gagaatctgc cgcagacgtt gctgcccaag acccctgaac
     3001 tctttgcgct ccggggcgga atccgcgttg gaggacattc gccgtctgag gcaggaatta
     3061 gtggattgct ctgccggtgc gcgggtagca tgaagtcagg agctgttcac ttgctttccg
     3121 attcgcctcc tgactttgaa gatctgcaag aggaacgaga cgccccgcgc gatcaacagg
     3181 agtaaacgat gcacccgcat tttgccaaaa cgcagtttga gacccgccgg cagtttctca
     3241 agcagtgcaa tactggcctg ggcgcgctcg cgctggccgc gctcgccggc aatagcgctc
     3301 ccgccgcgcc gctgccggat gtggacaatc cgctgtcgcc caaggcaccg ccgctgccgg
     3361 cgaaggtgaa gcgggtgatc tacctgcaca tgtccggcgc gccgccgcag cacgacttgt
     3421 gggactacaa gccgaagctg gtcgagcaca acctggagcc ctgcccggat gacctcttgg
     3481 cgattctgca gaaagagcgg ctgccgttca tcgacctcaa ggcccggcgg ccgaagatgc
     3541 tggggacgcc gtacaagttc gaaaagcgcg gccagtcggg cctggaaatg agcgacatct
     3601 ggaagcatct gccggaagtc gccgacgacc tgtgcgtcgt caagtcgatg acgaccgacc
     3661 agttcaacca cgccccggcg gagctgttcc tctataccgg cagcccgcgg aacggcgggg
     3721 cggcgatggg ctcgtggatt acctatggcc tgggcagcga gaaccaggac ctgccggggt
     3781 tcgtggtcct gatcagcggc ggaaccgacc cgaccggcgg caaagccctg tggagcaccg
     3841 gctttctgcc gtcggttttt cagggcgtgc agtgccggac agtcggcgag ccgatcctct
     3901 atgccaacaa ccccgatggg ctcagccgcg aggtccgccg ccgcagcctc gacgccctca
     3961 agcgcctcaa cgagctggaa ttgcaggaat tcggtgatcc ggaaacgctg acgcggatca
     4021 gccagtacga gctggcctac cggatgcaga tcgcggtgcc cgacgtgatg gacatcagca
     4081 aggaatcgaa ggaggtgctc gcagcctacg gcgccgagcc gggcaaggcg tcgttcgcga
     4141 acaactgcct gctggcccgc cggctggcgg agcagggcgt gcgatatgtg cagctctacg
     4201 actggggctg ggacatgcac ggcacggggg ccgacaacga tatcgtcacc ggcctgccga
     4261 agaaggtgaa ggacgtcgat cagccgatca ccgccctgat gaaggacctc aagcagcggg
     4321 ggatgctgag cgacacgctc gtcgtctggg ggggcgaatt cggccgcacg gccctcaacg
     4381 aagagcgcgg cggcagcaag ttcctgggcc gcgaccacca tccgcactgc ttcagcatct
     4441 ggctggccgg cggcgggatc aagcccggac actcctacgg cgcgaccgac gacctgggct
     4501 acttcgtcac cgagaacaag gtcggcatcc acgacctgca ggccaccatt ctgcacctcc
     4561 tcggcttcga cccccagcgc ttcagcttcc cgtaccaggg cctcaaccaa cgcctcatcg
     4621 gccccagcga cgagccgcgg gtagtgcagg agattttggc gtagggcgaa agtctgggcc
     4681 ggggagggtt acttggtact ctccttgatt ggtttttccg ccgtggcttt gacggtcaac
     4741 accgggcacc cggctttacg aacgacttct tcggcgacac ttcccatcag aacccgcatc
     4801 aggcccgtgc ggccgtgcgt cggcatgacg atcaggtccg cattctcctt ttcggccaga
     4861 tcgacgatgg cggtcgccgg cgagccgatc atcagcttgt gctcgcaggg cacctgggga
     4921 tcagtgggca cgacgtcgga gagcatccgc ttgagctctt cgcggtcggg ctcctggacg
     4981 ccgtagtaca attcgccgcc gccgtaagcc atcggcggct cttcgacgtg aacaatcacc
     5041 agcgtcgcac cgcggtcacg cgcaagcgaa gtggccattt ccagcgcggc ctggcccgct
     5101 gtcgagaaat cggttggaaa cagaatcttg gaggtattca tcgtgcgtct cctggtctga
     5161 attcgctacg ctcagcaccg accaagcggc tcttgcaccg cgagatttgt gcgccacaca
     5221 cttgcgagct aacccttctt ctcttcctaa cgcaactgca gtgccaaggc aagcaattgc
     5281 catccctgga tctgctccgg cctccagctg tctgccgttt cgctgagtcg tcgttgcggg
     5341 cgagccagac agcacctttg cgcattgcag cgccaagagg cgatagggta cgatcaggcg
     5401 cagtcactta gctctcgtac tggaacctgt ttatgcatgc ggttggcgca ctctggctgg
     5461 cgcttattcc tctggccgcg acggacggcg ccctcgattc cccggtcggc aggaagattc
     5521 agaatttcga gctgcacgat catcttggga cgccgcatgc tctcaaagac tgggccgaca
     5581 agcgggcggt cgtggtggta tttctcggaa ccgaatgtcc cctggcgaag caatacggcg
     5641 gtcggctggc cgagcttgcg gaacgatacc agtccaaaga ggtgcagatt atcggcatca
     5701 actcgaacca acaggattcg ctggctgaaa tcagtcactt cgtgcgggag cagaaaatc
//
LOCUS       DASUGB010000768         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1473619, whole genome shotgun sequence.
ACCESSION   DASUGB010000768 DASUGB010000000
VERSION     DASUGB010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473619"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..464)
                     /locus_tag="VFV87_14755"
     CDS             complement(30..464)
                     /locus_tag="VFV87_14755"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5105076.1"
                     /translation="MAEILDRLRPIAGRCVTRFHYFHYRGFFATQRKLLEKEEPKRAK
                     SLLYAYRVLLTGIHLLRIGQVEANLRRLNEQFRLPYIDDLIASKTGERTVSPALDWTF
                     HDAELARLDQQMADAFANSIMGETRDMGAVNDLLIELRLADD"
     gene            complement(1455..1871)
                     /locus_tag="VFV87_14760"
     CDS             complement(1455..1871)
                     /locus_tag="VFV87_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105077.1"
                     /translation="MAHETRTLHKLSPKFIIPVGTQVVLKAPKTLPGGEYRKPGTVGV
                     VVESPPHNEEPYVVQFADGSEAKARFPLVAGNISKGRVSSGLDSDWRRVDCRYHVRDV
                     LTPRIVAKVSAGSGNSESRFAFITLGPKNVQSYQSR"
     gene            2065..>3444
                     /locus_tag="VFV87_14765"
     CDS             2065..>3444
                     /locus_tag="VFV87_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105078.1"
                     /translation="MQRRDVLRVGSLSVAASVLPAAMLAGGRPAQAAAKSEPREGPGR
                     AKSVIFLWMAGGVTHIDSFDPKPDAPIEIRGILDDMPTNVPGTRFCETLPHLARVADK
                     LAVLRSYSHDSNDHLISQVYTLSGKKIANMTQLYSEPNIGSLVWKLQGPRNGLPGYIA
                     VPGITRPGPPPFNLFVGGWLGNAWRPYCLGGIQDIDFAVGEKLYDPPALATEDLKPQS
                     LGLPDEVTLARLSSRRQLRDSFDQALRTLDKNEKLLAADAQYENALRLLTTPQVRRAF
                     EVDDEPVAVREAYGRTKIGGRCLQARRLVEAGARFVMVDYGYDPEYGNIWDNHNAPVQ
                     NHPPIQQMVRRGYHLAGIDKAFAALINDLDERGLLQTTLVAFITEFGRTPQINGNGGR
                     DHWGAAGSLFFTGAGTKVGQVIGATDRTASKPVTHPYGPWDVAATIYQLLGVNYKSFA
                     YDIQDRPRPI"
BASE COUNT          661 a         1014 c         1122 g          647 t
ORIGIN      
        1 catggcttcc gccgcctgct gccgccgtat taatcgtcgg ccagccgcag ctcgatcagc
       61 aggtcgttca ccgctcccat gtcgcgagtt tcgcccatga tcgaattcgc gaaggcgtcg
      121 gccatttgct ggtcgaggcg cgcgagctcg gcgtcgtgga aggtccaatc aagcgcgggg
      181 ctgacggtgc gctcgccggt tttgctggca atgaggtcgt cgatgtaggg aaggcggaac
      241 tgctcgttca gccggcggag gttcgcttcg acctggccga tccgcagcag atgaatgccc
      301 gtcaggagga cacggtaagc gtacagcagc gacttggccc gcttcggctc ttccttctcc
      361 agcagtttcc gctgcgtggc gaagaagccg cggtagtgaa aatagtggaa ccgcgtgacg
      421 cagcggccgg cgatcggccg cagacgatcc agaatctctg ccagctaacc gctacgcagc
      481 gtcacctcgg atttttggga acgcataggt tcgctgtgtc cacagacgga caagcggtga
      541 gcgactggta tacttagggc cttccgggct acttgtggtg gcggagcggg cggtacgcaa
      601 cagtgaatcg aagtacgggt tcctcttctc tgctcagtcg acggagtttg gaattctggt
      661 aatcaccgtt cgggcgggcg gtcacttgca actatctccc tgacacggac cgagatcaga
      721 gaagaggaac ccgtactgga tgctgccggc gcgactgtct gggcacgaga atacgtcgcc
      781 agtccctcga accccggatg tccactgtct ggaatggcct tcgcgaggat accgtggcga
      841 ataatcagcg gacaacagca gttgtcaaga gtgccggacg caatcacaag gtttgcaaaa
      901 tctggtttgg ttctgacggc agctattacg ttaccgtgcc ctatcactcg gtgaacaagg
      961 cgctgatcac ccgggcgaca ttgaactatg cggccggtcc agtgcacacg gtccccgttt
     1021 cagacttgct cgaagtagca gcgttggacg acgaaaatcg gttaaagctg tcgcaccatc
     1081 ccgacggctt ctgtcagttt tctgggaaag gaatcctctc gggacgagat gaagatgggc
     1141 aacccaaagg cgttggaatc ttcgcgcgag cgctaaccca tgtccccaat ggaccgactt
     1201 tcggaatggt gttctacgga ttacatgaat tcgtcgcttg cgacaacgca ggagacgacg
     1261 acatcgtatt cgacgccgat ctcttggcac caatcccagg cggcaatgga tttcgcctcg
     1321 aagggtacta cttgcagcct gcacttcgaa ggttcgttga agtggacaat acgggcgcac
     1381 ggtggattag cctcatccat ccagctgctg caactctccg ccttagagtc gtactggcgc
     1441 ctgcgacgtg cgagctaccg gctttgatag gactgcacat ttttcgggcc gagtgtgatg
     1501 aaggcgaatc gggattcgct attaccggac ccggcggaaa ccttcgcgac aatgcgaggg
     1561 gtgagaacgt cgcggacatg atatctgcaa tctacccgcc tccagtcgga atccaagccc
     1621 gacgagacgc gacctttcga aatattgccg gcaactagag ggaagcgggc cttcgcctcg
     1681 ctgccgtcgg cgaattgcac gacgtacggc tcttcgttgt gcggcgggct ttcgacgacg
     1741 actccaaccg tccccggctt gcggtattcg ccgcccggca gcgtcttggg cgccttcagc
     1801 acgacctgcg tgccgacggg gatgatgaat ttcggcgaaa gcttgtggag cgtgcgcgtt
     1861 tcgtgggcca tggccagtcc tccgcaacca gattagcgga cggcaagact ggacacgcgg
     1921 ccagaggctg cggttcagcg attccttgcg gcattccgct tgatgttgca cccagatcga
     1981 ccgataatcc gtcactgatg gattgttcga ctgcggggaa cgtgccatat gattgcagac
     2041 cgtcaacctg gtctgggagg cctcatgcag cggcgggacg tgctgcgcgt gggatcgctg
     2101 agcgtggcgg cgagcgtgct gccggcggcg atgctggccg gcggcaggcc ggcacaagcg
     2161 gcagccaaga gcgagccgcg ggaagggccg ggccgggcga agagcgtcat cttcctgtgg
     2221 atggccggcg gcgtgacgca catcgactcg ttcgatccca agcccgacgc gccgatcgaa
     2281 atccgcggca tcctggacga catgccgacc aatgtgccgg gcacgcggtt ttgcgagacg
     2341 ctgccgcacc tggcgcgggt cgccgacaag ctggcggtgc tgcggagcta ttcgcacgac
     2401 agcaacgacc acctgatcag ccaggtctat acgctctcgg gcaagaagat cgcgaacatg
     2461 acgcagcttt acagcgagcc caacatcggc tcgctggtgt ggaagctgca ggggccgcga
     2521 aacgggttgc cgggatatat cgccgtgccg ggaattacgc gtcccggtcc cccgccgttc
     2581 aacctgttcg tcggcggctg gctggggaac gcgtggcggc cgtattgcct gggcggaatc
     2641 caggacatcg atttcgccgt gggcgagaag ctctatgatc cgcccgccct ggcaacggag
     2701 gacctcaagc cccagtcact cggcctgccg gacgaagtca cgctggcccg tctttccagc
     2761 cggcggcagt tgcgcgattc gttcgatcaa gcgctgcgga cgctggataa gaacgagaag
     2821 ctcctggcag ccgatgcgca gtacgagaac gccctgcgac tcttgacgac gccccaggtg
     2881 cggagggcgt tcgaggtcga tgacgagccg gtggcggtcc gcgaggcgta tggccggacg
     2941 aagatcggcg ggcggtgcct gcaggcgcgg cggctggtcg aggcgggggc ccggttcgtg
     3001 atggtcgatt acggctacga cccggaatac ggcaacatct gggacaacca caacgcgccg
     3061 gtgcagaacc atccgccgat ccagcagatg gtccgccggg gctatcacct ggcgggaatc
     3121 gacaaggcgt tcgcagccct gattaacgat ttggacgagc gcgggctttt gcagacgacg
     3181 ctggtcgcgt tcattaccga gtttggccgc acgccgcaga tcaacggcaa tggcgggcgc
     3241 gatcactggg gcgcggcagg ctcgctgttc tttaccgggg cggggaccaa agtcggccag
     3301 gtgatcggcg cgaccgaccg caccgccagc aagccggtga cgcatcccta cggcccgtgg
     3361 gacgtggcgg cgacgatcta tcagcttttg ggtgtgaatt acaagagctt tgcgtacgat
     3421 atacaagatc ggccgcggcc gatc
//
LOCUS       DASUGB010000769         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1473633, whole genome shotgun sequence.
ACCESSION   DASUGB010000769 DASUGB010000000
VERSION     DASUGB010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473633"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1418)
                     /locus_tag="VFV87_14770"
     CDS             complement(210..1418)
                     /locus_tag="VFV87_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX5105079.1"
                     /translation="MHHKGFLHSGHWPTLLASFLYFDISFMVWGLLGVLVNYIVADLP
                     LSASQKGFLIATPILGGALLRLPMGILADRWGGKRAGILGMVLTIVPLALGWLWADTY
                     GELLFAGFLLGMAGASFAVALPLASRWYPLEHQGLALGIAGAGNSGTVIAALLAPRLA
                     EVIGWQAVLAVAAACVLATLIAFCLLAKDSPDQPAPRKLVEYAAPLSRRDCWRFCAFY
                     CVTFGGFVGLANFLTLFFHEQYEMSRVTAGTLTALCVFSGSMLRPVGGYLADRLGGAR
                     LLLVVFAAVAITMGGVAVLPPLAMAVTLMVVAMGLLGTGNGIVFQLIPQRFQREIGVV
                     TGIVGAAGGVGGFILPSVLGLAKQWTGSFSLGLAGLSAVAFVSAGAVWSASRAWRLAT
                     QERLEPAMSS"
     gene            complement(1463..>3508)
                     /locus_tag="VFV87_14775"
     CDS             complement(1463..>3508)
                     /locus_tag="VFV87_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX5105080.1"
                     /translation="TPDRLTQPHLIHNGQLRPATWDRTLDFAARRLKEIIAAHGPDSV
                     AFYGSGQLDTETVYLVGKLFKGNLGTNNTDSNSRLCMASAVAGYRTSLGSDGPPTCYD
                     DIEAADVILVIGSNMAEAHPVTFDRIRDRRRKSRPPTPSGSDRTANVVPDAGRSRSER
                     PTLIVIDPRRTATAEAADLHVPIAPGGDIALLNAIGRRLLTAGRVDEVFLSDHTAGFE
                     EYAAFLIEQDEDELLAACGVSPETLEQLADAIGSAKGWLSFYCMGLGQSTVGLWKHNA
                     LINLHLLTGQIGKPGAGPFSLTGQPNAMGGREAGLLAQQLPGYRFVDDAEHRREMETH
                     WTLPAGSISTKPGLTAVEMFRALETGKLKAIWIAATNPLVSLPDLHQTRRALAKAELV
                     IVQDAYHPTETTQHAHILFPAASWAEKEWTSTNSERMVSFSEKLIAPPGSSRPDWEIV
                     AGLAKRMGFAGFEFAHRDEVWNEFIRCTAGRPCDMVGMTADRLRRAKGLQWPCPDVAH
                     PGTQRRYLDRRFPTPDGRARFWPRPHQGPRETTDHEFPLALTTGRLYAHWHSLTRTGK
                     STKLMKRDPAPYIEIHPGDAERLMIADGTLVQVTSRRGTIRLAARLCDSLRPGMVFVP
                     FHWGDLWSEGNAANYLTISAIGRIAKQPELKFCAVHVEPVSRTQEPRTEEPKNQGRG"
BASE COUNT          591 a         1110 c         1242 g          565 t
ORIGIN      
        1 gactatcggc cggcacggtc ggcggactgg tgcgaacgtc ggtccacagg tcttcgagcg
       61 cgccctggcc gcgcgacggt gcggcaagcg ccgcacatag cgcagcgctc agcagcatgg
      121 ccaataagtt gatgccccgc attccccacc tcgcggcggg aggctatcaa cctgcggccg
      181 tgcgatcaag agaaacgcgt ggtccgctgc tagctcgaca ttgctggctc caggcgctct
      241 tgcgtcgcca gtcgccacgc gcgggaagcg ctccagaccg ctccggcgga gacaaaggcg
      301 acggcggaca gcccggctaa gcccaggctg aacgagccgg tccattgctt ggcgaggccg
      361 aggacgctcg gcaggataaa cccgccgacg ccgccggccg cgccgacgat ccccgtgacg
      421 acgccgattt cgcgctggaa gcgctgtgga atgagctgaa acacgatgcc gttgccggtg
      481 ccgagcagac ccatcgcgac gaccatcaac gtgacggcca tcgccagcgg cggcagcacc
      541 gcaacgcccc ccatcgtgat ggcgactgcc gcgaatacaa ccagcagcag ccgcgctccg
      601 cccaggcgat cggccagata gccgccgacc ggccgcagca ttgagccgct gaagacgcac
      661 agggcggtga gcgtgccggc cgtcacgcgg ctcatttcgt attgctcgtg gaagaagagc
      721 gtcaggaaat tcgccaggcc cacaaagccg ccgaacgtca cacagtagaa cgcacagaac
      781 cgccagcaat cgcggcgcga gagcggagcg gcgtattcga ccagcttgcg cggcgccggc
      841 tggtcggggc tgtctttggc gagcaggcag aaggcgatga gcgtcgcgag cacgcaggcc
      901 gccgcgaccg ccagcaccgc ctgccagccg atcacttcgg ccaggcgcgg ggcgaggagc
      961 gcggcgatca ccgtcccgct gttgcccgct ccggcaatgc ccagagccag gccctgatgc
     1021 tccagcggat accagcggct ggccagcggc agcgcgacgg cgaagctggc cccggccatt
     1081 cccagcagaa atccggcgaa gagcaactcg ccgtaggtat cggcccacag ccagccgagc
     1141 gccagcggca cgatcgtgag caccatgccc aggatgcccg cccgcttgcc gccccagcga
     1201 tcggcaagaa ttcccatcgg cagccgcaag agcgcgccgc cgaggatcgg cgtggcgatc
     1261 aggaaaccct tctggctggc cgagagcggc aagtcggcca cgatgtaatt caccagcacg
     1321 ccgagcagcc cccagaccat gaagctgatg tcgaagtaca ggaacgaggc gaggagcgtg
     1381 ggccagtggc cgctgtggag gaagcctttg tggtgcatgg gagttagaaa tttcaagaac
     1441 cgaagaattc aagaaccaaa aatcatcctc ggccttggtt ctttggttct tcggttcttg
     1501 gttcttgggt tcgggacacg ggttcgacgt ggacggcgca gaatttcagt tcgggctgct
     1561 tggcgatgcg gccgatggcg ctgatcgtca ggtagttcgc agcgttgcct tcgctccaca
     1621 ggtcgcccca gtggaacggc acgaacacca tgcccggccg cagcgaatcg cagaggcgcg
     1681 ccgccaggcg gatcgtgccg cggcggctgg tgacctgaac cagcgttccg tcggcgatca
     1741 tcagccgctc ggcgtcgccc ggatggattt cgatgtacgg ggccggatcg cgcttcatca
     1801 gcttggtcga tttgccggtg cgcgtgaggc tgtgccagtg ggcatacagg cggccggtcg
     1861 tgagggccag agggaactcg tgatcggtcg tctcgcgcgg gccttggtgc ggccgcggcc
     1921 aaaagcgggc ccggccgtcc ggcgtcggga accgccggtc gaggtaccgc cgctgcgtgc
     1981 cgggatgggc cacgtcgggg cacggccact gtaggccctt cgcgcgccgc aggcggtcgg
     2041 cggtcatccc caccatgtcg cacgggcgtc cggcggtgca gcgaatgaac tcgttccaga
     2101 cttcgtcgcg gtgtgcgaac tcgaagccgg caaagcccat ccgcttcgcc aggccggcga
     2161 cgatttccca atcgggccgc gaactgccgg gaggagcgat caatttctcg ctgaagctga
     2221 ccatccgctc gctgttggtg ctggtccatt ccttctcggc ccagctcgcg gcgggaaaga
     2281 gaatgtgggc gtgctgcgtc gtttcggtcg ggtgataggc gtcctggacg atgaccagct
     2341 cagctttggc cagggcgcgg cgggtttgat gcaggtcggg caggctgaca agcggattgg
     2401 tcgccgcgat ccagatcgct ttgagcttgc cggtctccag ggcacggaac atttcgaccg
     2461 cagtcaggcc gggctttgtc gagatgctgc cggcgggcag ggtccagtgc gtctccatct
     2521 cgcggcggtg ctcggcgtca tcgacgaacc gatatccggg caactgctgg gcgaggaggc
     2581 ccgcttcgcg gccccccatc gcgttgggct ggccggtgag cgagaacggc ccggcgcccg
     2641 gtttgccgat ttgcccggtg agcaagtgca ggttgatgag ggcgttgtgc ttccacaggc
     2701 cgaccgtgct ctggccgagg cccatgcagt agaagctgag ccagcccttc gcactgccga
     2761 ttgcgtccgc gagttgctcg agcgtttccg gcgatacgcc gcaggcggcg agcagctcgt
     2821 cttcgtcctg ctcgatcagg aaggcggcat attcctcgaa gccggcggtg tgatcgctga
     2881 ggaatacctc atcgacgcgg ccggctgtca gcaggcggcg gccgatggcg ttgagcaggg
     2941 cgatgtcgcc gccgggagcg atcggcacgt gcaggtcggc tgcttcggcg gtggccgtgc
     3001 ggcgggggtc gatgacgatt aaagtaggcc gctcactccg tgagcggcca gcgtctggga
     3061 cgacgttagc ggtgcgatcg gaccccgacg gagtcggggg cctacttttg cggcggcggt
     3121 cgcggatgcg gtcgaacgtg accgggtggg cctcggccat gttcgagccg atgacgagga
     3181 tcacgtcggc tgcttcgata tcgtcgtagc acgtgggcgg gccgtcggaa ccgagactcg
     3241 tgcgatagcc ggccaccgcg gaggccatgc acagccggct gttgctgtcg gtgttgttgg
     3301 tgccgaggtt gcccttgaag agcttgccga cgagatagac cgtctcggtg tcgagctggc
     3361 cgctgccgta aaaagcgacg gagtcgggtc cgtgtgcagc gatgatctcc ttgagccggc
     3421 gggcggcgaa atcgagcgtc cggtcccacg tggcgggccg caactggccg ttatggatca
     3481 ggtgcggctg cgtgaggcga tcgggcgt
//
LOCUS       DASUGB010000770         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1752275, whole genome shotgun sequence.
ACCESSION   DASUGB010000770 DASUGB010000000
VERSION     DASUGB010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752275"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VFV87_14780"
     CDS             complement(<1..1312)
                     /locus_tag="VFV87_14780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105081.1"
                     /translation="MSEEFTEQQSPADLQRSQELSLERTRPPAEVPGYSSQRFLGSGA
                     YGEVWVGACRNTGRKVAIKFYAHKRGVDWSLLAREVEKLRFLSADRYVVQLLDVGWEA
                     DPPYYVMEYVENGSLDDLLARHGTFSVPEAVEMFREIAVGLSHAHGKGVVHCDLKPAN
                     ILLDQDHRPRLADFGQSRLSHEQKPALGTLFYMAPEQADLEASPDVRWDVYALGAILY
                     TLLVGSPPHRNDEVADRIAASQDLGDRLARYRHAIRSAPPPAEHRKVPGLDKALAEII
                     DRCLAANPDDRFANVQEVLDALTARSRARERLPMLFLGFIGPLIVLLVTALFSWRGYL
                     QAVQNAEAEYRGLAIENNQFAADLAAAKVTSEMGRYFRLAEEEANQGEFLPYFFQVVD
                     ASPTLARLSDPQVVEQEIDTLRAKFFEEVDRRTLDRYLQERLKSY"
     gene            1625..2683
                     /locus_tag="VFV87_14785"
     CDS             1625..2683
                     /locus_tag="VFV87_14785"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5105082.1"
                     /translation="MLRVPATKLTRARFPVVDVHTHFRQKFRGTADELDAWVKLMDRN
                     QIAVCVSLDGQWGELVDEHAKLLWTKHKDRFVIFGNIDWQGGGKADDPASWDCNRPDF
                     ARRVARQLAADKERGICGIKIFKGFGLEYKNADGSLIKIDDPRFDPIWQACGELGLPV
                     LIHVADPLAFFQPIDETNERWEELHRRPDWSFFGPQFPEHKELLAAFLRVVERHPQTT
                     FIGAHAVSNAEDLATLGRWLDDHPNLNLDTASRISEFGRQPFTARKFFLKHQDRILFG
                     TDGPWPEVRLQLYWRFLETEDEYFPYSEKPFPPQGLWNIYGLNLPDEVLRKVYHENAA
                     RIVPGIKERMQQWKQKDY"
     gene            2771..3322
                     /locus_tag="VFV87_14790"
     CDS             2771..3322
                     /locus_tag="VFV87_14790"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5105083.1"
                     /translation="MIQRLDRPATIADLQKVPEKAELVKGAIVIMSPASAGHGRKAGR
                     IYQSLLEYEERTGKGIALPDNVGFIVDLPNRQSFSPDAAYFLGPYHENEFIDGAPAFA
                     AEVRSPEDFGPAAEREMADKRADYFAAGTQVVWDVDMIRDKVVRVYRADTPDQPVIYG
                     TADIAEAEPILPGWRLLVAELLK"
     gene            3410..4000
                     /locus_tag="VFV87_14795"
     CDS             3410..4000
                     /locus_tag="VFV87_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105084.1"
                     /translation="MSTAPSKIPVACPQCGKNLMVPASVAGKQGRCPACQHVFVIPAP
                     PVFDEDDLEPIPDLAPLDNGPYAPLAADPFAPSAGNPFAGTTGGNNAMQPAQPQGYHP
                     NYQATAQPQHALANQYMANAAASQQYDPYAAKKSDTDSDWGINAGIGGGLLLMIGAVV
                     WFFVALLFFDVIFFYPPIMFIIGLVAFFRGIAGKFS"
     gene            4107..>4450
                     /locus_tag="VFV87_14800"
     CDS             4107..>4450
                     /locus_tag="VFV87_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5105085.1"
                     /translation="MSLLYMTQAPPPAPAPSPDDAGLHEAARRVKLQKLIDLGVDPWG
                     GRLDDRQLIGDIRARQGEIRFATESGKQVEVPSREANPELDFRGWLQDQGKGDWQGPK
                     VRAAGRIVLSRDA"
BASE COUNT          838 a         1362 c         1417 g          833 t
ORIGIN      
        1 gatagctctt taaacgctcc tgcaaataac gatccagcgt ccgccgatcg acctcctcaa
       61 aaaactttgc acgcagcgtg tcgatctcct gctcgacaac ctgcggatcc gagagccggg
      121 cgagcgtagg acttgcatcg acgacttgga aaaagtacgg caggaattca ccttggttgg
      181 cttcttcctc ggccagccga aagtaccgtc ccatttcgct ggtgaccttg gccgcagcca
      241 ggtcggcggc aaactggttg ttctcgatcg ccaacccgcg atactccgct tccgcgttct
      301 gcactgcctg gagatagccg cgccaactga aaagggccgt gacgagcagc acaatgagcg
      361 ggccaatgaa gcccaaaaag agcatcggca ggcgctcgcg ggcgcgggac cgggcggtga
      421 gggcgtcgag tacctcctgc acgttggcga aacggtcgtc cgggttggcg gccaggcagc
      481 ggtcgatgat ctcggccagg gccttgtcca ggccggggac cttgcggtgc tcggctggcg
      541 gcggggccga gcggatcgcg tgccggtacc gcgccagccg gtctcccagg tcctggctcg
      601 cggcgatccg gtcggcgacc tcatcgttgc ggtgcggcgg cgagccgacc agcaacgtgt
      661 agaggatcgc gccgagcgca tagacgtccc accgcacatc gggcgacgct tccaggtccg
      721 cctgctcggg cgccatatag aacagcgtcc ccagggccgg cttttgctcg tgcgagaggc
      781 gcgactggcc aaaatccgcc agccgcggcc ggtggtcctg gtcgagcagg atgttggccg
      841 gcttgaggtc gcagtggacg acccccttgc cgtgagcgtg cgagaggccg acggcgatct
      901 cgcggaacat ttccacggct tccggcacgc tgaacgtgcc gtgccgtgcg aggaggtcgt
      961 cgagcgaacc gttctcgacg tactccatta cgtaataggg cggatcggct tcccagccga
     1021 cgtctaaaag ctgcacgaca tagcggtcag ccgacagaaa gcggagcttt tcaacctccc
     1081 gcgccaagag cgaccaatcg acgccgcgct tgtgggcgta gaacttgatc gccaccttgc
     1141 ggcccgtatt gcggcaggcg ccgacccaga cctcgccata ggctcctgag ccgaggaacc
     1201 gctgcgagct gtagccgggc acctcggcgg gggggcgcgt ccgctccagg ctcagttcct
     1261 gggagcgctg caaatcggcg ggcgattgct gctcggtgaa ttcttcggac ataacgggca
     1321 agcgacgggc gaagcgaata tggttagcgt atctgattct atacggcggg ccgggaggcg
     1381 tcttgtgatg aataaccaag caccaagcat caagcaccaa acaagcatcg aattccaagg
     1441 ctggaatgat cgaaacgccg acgtgaatcg gtttgggata tgcatgcctt ggtgcttcgg
     1501 atttatttgg tgcttgttgc ttttgatgac gtgcttctgt ccgttgtcgc tagcaggcca
     1561 ggaattgccc ctcgatggtc gcgagggccg aaatctgctt ttggaaaatt tccgcccgcg
     1621 gtcgatgctc cgcgtgcccg cgacgaagct gacgcgagcc aggttcccag tagtcgatgt
     1681 tcacacgcat ttccgccaga agttccgtgg cacggcggat gagctggatg cgtgggtcaa
     1741 gctgatggac cgcaaccaaa tcgctgtctg cgtgagcctc gatggccagt ggggcgagct
     1801 ggtcgatgag catgcgaagc ttctgtggac gaagcacaag gaccggttcg tcattttcgg
     1861 caacatcgac tggcaaggcg gcggcaaggc cgacgatcca gcgagttggg actgcaaccg
     1921 gcccgatttc gcccgccgcg tcgctcgcca gcttgccgcc gacaaagagc gcggcatttg
     1981 cggcatcaaa atcttcaagg ggtttggcct ggagtacaag aacgctgacg gctcgttgat
     2041 caagattgat gatccgcggt tcgatccgat ctggcaggca tgcggcgagc tgggcctgcc
     2101 ggtgctgatc cacgtggccg atccgctggc cttttttcag ccgatcgacg aaaccaacga
     2161 gcgctgggag gagttgcacc gccggcccga ttggagcttt tttgggccgc agtttcccga
     2221 gcacaaagag ctgctcgccg cctttctgcg agtggttgag cggcatccgc agacgacgtt
     2281 catcggcgcg cacgccgtca gcaatgctga agacctcgcc acgcttggcc gctggctcga
     2341 cgaccatccc aacctcaatc tcgacaccgc cagccgcatt tccgaattcg gccgccagcc
     2401 gtttaccgct cgcaagttct tcctcaagca tcaggaccgc atcctcttcg gcaccgacgg
     2461 cccctggccg gaagtacggc tgcaactgta ttggcggttt ctcgaaacgg aggacgagta
     2521 cttcccctac tccgaaaagc ccttcccgcc gcagggcttg tggaatatct atggcctgaa
     2581 tctgccggac gaagtactcc gcaaggtcta tcacgaaaat gcggcgcgga tcgtgccggg
     2641 gattaaagag cgcatgcagc aatggaagca aaaagactac tgatgccccc gcgtcccgtg
     2701 gtttggccgg gcaatcgtgg cgtgatagga tacgaggaag gaacagtgag gagcgggagg
     2761 agcggttgcc atgatccaac ggcttgatcg tccggctaca atcgcggacc tgcaaaaggt
     2821 tccggagaag gcggagctgg tcaagggagc aatcgtcatc atgtcgcctg ccagcgcggg
     2881 gcatggtcgc aaggccggcc gcatttacca gagcctgctg gagtacgaag agcggacggg
     2941 gaagggaatt gctctgccag acaacgtcgg tttcatcgtg gatttgccga atcgccagtc
     3001 cttctcgcct gacgccgcct atttccttgg gccgtaccac gaaaatgaat tcatcgacgg
     3061 ggcgcccgcc tttgccgcag aagtgcgcag cccggaggac ttcggtcctg ccgcggaacg
     3121 cgaaatggcc gataagcggg ccgactactt tgccgcaggg acgcaagtcg tttgggatgt
     3181 cgatatgatt cgcgacaagg tcgttcgcgt gtaccgtgca gacactcccg atcagccggt
     3241 catctatggc actgcggata ttgctgaagc cgagccgatt ctgcctggct ggcgattgct
     3301 cgtggcggag ttgctgaagt aacggcgcgc aactgggagt tggcgttcgg cggccgctga
     3361 cggtatcatc cagcgattgg ctggacctcc ttcggcagga gcgaatcgga tgagcaccgc
     3421 cccaagcaag atccccgttg cgtgtccgca gtgcggcaag aacttgatgg tcccagcgag
     3481 cgtcgctggc aagcaggggc ggtgcccggc ttgtcagcac gtgtttgtga ttccagcgcc
     3541 gcccgtattc gacgaagacg acctggagcc aattccggat ttggcgccgc tcgataacgg
     3601 cccgtatgca ccgctggctg ccgatccctt cgccccatcc gccggcaatc cgttcgctgg
     3661 cacgacaggc ggcaacaacg ccatgcagcc ggctcagccg cagggatatc atccgaacta
     3721 ccaggcgacc gcccagccgc aacatgccct ggcaaaccag tacatggcca atgccgccgc
     3781 gagccagcag tacgatccct acgccgcgaa aaagagcgac accgatagcg actggggcat
     3841 caatgcgggc atcggcggcg ggctgctcct gatgatcggg gcggtggtct ggttctttgt
     3901 cgccctgctg tttttcgatg tgatcttttt ctacccgccg atcatgttca tcatcggcct
     3961 ggtcgccttc ttccgcggga ttgcgggcaa gttcagctaa gcggtggcgg tagtggtgta
     4021 ctgagtggcc gccctgttgg cgtttgccgc cgcgcgctag aatcgggcct tcatccactt
     4081 cccgctgaac cgccggcatt gggccggtgt ctcttctgta tatgacgcaa gctccgcctc
     4141 ccgcgcccgc cccctctccc gatgatgccg gcctgcacga agcggcccgc cgcgtcaagc
     4201 tgcagaaact gatcgacctg ggcgtcgatc cctggggagg ccggctggac gaccggcaac
     4261 tcatcggcga tatccgcgct cgtcagggtg aaatccgctt cgccaccgag agcggcaaac
     4321 aggtggaagt gcccagccgc gaggccaacc ccgagctcga cttccgcggc tggttgcagg
     4381 atcagggcaa gggggattgg caggggccga aggtccgagc cgccggacgg atcgttctct
     4441 cgcgcgatgc
//
LOCUS       DASUGB010000771         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_502821, whole genome shotgun sequence.
ACCESSION   DASUGB010000771 DASUGB010000000
VERSION     DASUGB010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502821"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1201
                     /gene="mqnC"
                     /locus_tag="VFV87_14805"
     CDS             65..1201
                     /gene="mqnC"
                     /locus_tag="VFV87_14805"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEX5105086.1"
                     /translation="MNIQSILDKAAAGQRLTPAEGLALLESRELTAIGRAADAVTRRL
                     HPKPIRTYNIDRNINYTNVCTAVCDFCAFYRKPKSPEGYVLPREELLQKVRETVELGG
                     DQILMQGGLHPDFDLAWYEELLADIKSHFPQVNLHAFSPPELHAFTKINKLPIRTVLE
                     RLKAAGLGSIPGGGAEILVDRVRKEITRGKVLTDDWLNVMRVWHELGGVSTVTMMFGH
                     VETLAERIEHLERVRQLQDETGGFTAFICWTFQPEHTDLAHIPPAGSFDYLKTQAVAR
                     LYLDSVPNIQSSWVTQGLKIGQLALLFGANDMGSLMIEENVVAEAGTVHYLTLEQIRG
                     AIEELGFTPRQRNVRYELFPLEHERRAIEANRKRQELPLVQLAG"
     gene            1231..1692
                     /locus_tag="VFV87_14810"
     CDS             1231..1692
                     /locus_tag="VFV87_14810"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5105087.1"
                     /translation="MPHGSVSEVIPASASEVFDLLHDYDRRLEWDTLLSAAYLTDGCE
                     QAELGASSVCVGRGSLGRIALKTTYVTFDRPRLAAVKMVNRPPFFACWAASIRHDELG
                     PDESRVTYSWTFTARPRWLRWLLEPVMNRIFRWETRKRLAALSRFFRAGCG"
     gene            1774..3036
                     /locus_tag="VFV87_14815"
     CDS             1774..3036
                     /locus_tag="VFV87_14815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5105088.1"
                     /translation="MTTNSYAPTVSGVVRSVQTVSAALRRLGHQVLIIAPEQDGGPEE
                     EHVVRLPSIQHITANDYAIRLPIPGYLTRTLDAFRPDVIHTHHPLLLGGTALRVAAGR
                     RLPVVFTHHTMYEHYMHYLSGDTAALERFAARLATEFANLCDHVIAPSQSVADVLQER
                     GVESPITVIPTGVDTAAFTTSRGVKTRRQLAVPEEAFVVGHVGRLAPEKNLAFLQSAV
                     IEFLQSHPHAHFVFAGSGPSSDDLLRAGADAGLADRWHALGLLEKRELTDAYTAMDVF
                     VFASHSETQGMVLAEAMAAGVPVVALDASGVREIVQDQVNGRLLVGERQATFVTALHE
                     VSNATTARRSAMRRAACKTAAEYSIDRCVEKLACVYQHVAEEKSRSRQSDAVWPAALR
                     RFDEEVRIWTGVSRAVADSMMGSQQAAQ"
     gene            complement(3071..>3405)
                     /locus_tag="VFV87_14820"
     CDS             complement(3071..>3405)
                     /locus_tag="VFV87_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5105089.1"
                     /translation="SGGERNRVHLAKLLRRGSNVLLLDEPTNDLDVDTLRALEEAILN
                     YAGCVVVISHDRWFLDRIATHILAFEGEGYVHWCEGNFQTYEEQRKQRLGIAADQPHR
                     FRYKKLAK"
BASE COUNT          630 a         1118 c         1046 g          611 t
ORIGIN      
        1 caccctcacc ctagccctct cccaaaggga gagggaactg gtcgtcggca gaaaacgtac
       61 cccaatgaat atccaatcca tcctcgacaa agccgccgcc ggccagcgcc tcacgcccgc
      121 cgaaggcctc gccctgctcg aatcgcgcga gctgaccgca atcgggcgcg cggcggatgc
      181 cgtgacccgc cggctgcatc ccaagccgat ccgcacgtac aacatcgacc gcaacatcaa
      241 ctacacgaac gtctgcacgg cggtctgcga tttctgcgcc ttctatcgca agccgaagtc
      301 gccggagggc tacgtcctgc cgcgcgagga actgctgcag aaggtgcgcg agaccgtcga
      361 gcttggcggc gatcaaattc tgatgcaagg cggcttgcac ccggacttcg acctggcgtg
      421 gtacgaggaa ttgctggccg atatcaaatc gcacttcccg caagtcaacc tccacgcatt
      481 cagtccgccc gagctgcacg ccttcaccaa aatcaataag ctgccgatcc gtacggtgct
      541 cgagcgcctg aaggcggctg ggctagggag cattcccggc ggaggggcgg aaatcctcgt
      601 cgatcgcgtc cgcaaggaga ttacccgcgg caaagtgctc accgacgact ggctgaacgt
      661 gatgcgcgtc tggcacgaat tgggcggcgt gagcacggtg acgatgatgt ttggccacgt
      721 cgaaacgctc gccgagcgga tcgagcatct ggagcgcgtc cgccaattgc aggacgaaac
      781 cggcggcttc acggcgttca tttgctggac cttccagccg gaacacaccg atctggccca
      841 catccccccg gccggctcgt tcgactacct caaaacgcag gcggtagcgc ggctgtatct
      901 cgacagcgtc cccaatattc agtcatcttg ggtcacgcaa ggactgaaaa tcggccagct
      961 cgccctcctc ttcggcgcta acgacatggg gagcctgatg atcgaggaaa acgtcgtcgc
     1021 cgaagccggc accgtccatt acctcacgct cgaacagatt cgcggcgcga tcgaggaact
     1081 cggcttcacg ccccgccagc ggaatgtgcg ctacgagctg ttcccgctgg agcacgagcg
     1141 ccgcgcgatc gaagccaacc gaaagcggca ggagcttcca ctggtgcagt tggcgggttg
     1201 atcgcacgag aattagagag gcgagtaccg atgcctcacg gctccgtctc ggaagtcatt
     1261 cccgcatcgg ccagtgaggt cttcgacctc ctgcacgact acgaccggcg gttggagtgg
     1321 gacaccctgc tctcagcggc atatctgaca gacggctgtg aacaggcaga actgggcgcg
     1381 tcgagcgtct gcgtcggccg cggttcgctg ggcaggatcg cgctcaaaac gacgtacgtc
     1441 acgttcgacc gcccgcgcct cgccgcggtg aagatggtca accgtcctcc cttctttgca
     1501 tgctgggccg cgtcgattcg tcacgacgaa cttggccctg acgagtcgcg cgtcacctac
     1561 tcctggacct tcacggcccg cccgcgctgg ctgcgctggc tcctggagcc ggtcatgaac
     1621 cgcatcttcc ggtgggaaac ccgcaagcgg ctggcagcgc tgagcagatt tttccgagcc
     1681 gggtgcggct gacttgcgcc gattccggca ccgtatttgc tgcccttctc ggtgcaatat
     1741 gttcgcgggc aaaatcgcac catgaacatc gtgatgacga ccaattcgta tgcccccacc
     1801 gtctcgggcg tcgtccgctc ggtgcagacg gtctcggcag cactccgccg gctgggacat
     1861 caggtcctga tcatcgctcc cgagcaggac ggcggacctg aggaagagca cgtcgtgcgg
     1921 ctgccatcga ttcaacacat caccgccaac gactatgcaa ttcgcctgcc gattcccggc
     1981 tatctgacgc gcacgctcga cgcgtttcgg cccgacgtca ttcacacgca tcatccgctg
     2041 ctgttgggcg gtacggcgct tcgcgtggcg gccgggcgca ggctgccggt cgtgttcacc
     2101 caccacacga tgtacgagca ctacatgcac tatttgtcgg gcgacacggc ggccctggaa
     2161 cggttcgcgg ctcgcctggc gacggagttc gccaatcttt gcgaccacgt gatcgcgccg
     2221 agccagagcg tcgccgacgt cctgcaagag cggggggtcg aatcgccgat cacggttatt
     2281 ccgaccggcg tcgataccgc cgcattcaca accagccggg gtgtgaaaac gcggcggcag
     2341 cttgcagttc cggaggaggc atttgtcgta gggcacgtcg gtcgcctggc cccggaaaag
     2401 aacctggctt tcttgcaatc ggcggtgatt gagttcttgc aatcacatcc ccacgcgcat
     2461 ttcgtgttcg ccggcagcgg cccatcgagc gacgatctcc tgcgggccgg cgcagacgcc
     2521 gggttggccg atcgctggca cgcgctgggg ctgctcgaaa aacgtgagct gaccgatgcc
     2581 tataccgcga tggacgtctt cgtttttgca tcgcactccg aaacgcaagg catggtactg
     2641 gccgaagcga tggccgccgg cgttccagtc gtcgccctcg atgccagcgg cgtccgtgag
     2701 atcgtgcagg accaggtaaa cggccggctg ctggttgggg aacgtcaagc gactttcgtc
     2761 acagcattgc acgaggtctc caacgcaacg accgcacggc gctccgcaat gcgccgggcg
     2821 gcctgcaaga ccgccgcgga atactcgatc gaccgctgcg tcgagaagct tgcctgcgtc
     2881 tatcagcacg tcgccgagga aaagtcgcgt tcgaggcagt ccgatgccgt ctggccagcg
     2941 gctctgcggc gattcgatga ggaagtcaga atctggaccg gcgtaagccg cgcggtcgcc
     3001 gattcgatga tgggctcgca acaagctgcc cagtgaatgc cgtgcgatac ttgccgttac
     3061 ccgccggacc ctacttcgcc agcttcttat accgaaaccg gtgcggctgg tcggcggcga
     3121 tgccgaggcg ttgcttccgc tgctcttcgt aggtctggaa gttgccttcg caccagtgga
     3181 cgtagccttc gccttcaaag gccaggatgt gcgtcgcgat gcgatcgagg aaccagcggt
     3241 cgtggctgat cacgaccacg cagccggcgt agttgaggat cgcctcttcc agggcccgca
     3301 gcgtatcgac atccaggtcg ttcgtcggct cgtcgagcaa cagcacgttg ctcccgcggc
     3361 ggagcagctt ggccaggtga acgcggttgc gctcgccgcc cgaca
//
LOCUS       DASUGB010000772         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2030455, whole genome shotgun sequence.
ACCESSION   DASUGB010000772 DASUGB010000000
VERSION     DASUGB010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030455"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFV87_14825"
     CDS             complement(<1..491)
                     /locus_tag="VFV87_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HEX5105090.1"
                     /translation="MKTCLLACLLSIGPAASALAQTAAPIGPSSPLPSPAPPLLTDPV
                     SEAIRGGPESWTSPQRLSSTLQVMLLLTVLSLAPAVLLMTTSFVRIIVVLGLLRQALG
                     TQQLPPSQVITALAMFMTLVVMTPTWNEIYSQSIRPYSRGEITDPEEAFNAGAQPLKE
                     FMAR"
     gene            complement(488..1120)
                     /locus_tag="VFV87_14830"
     CDS             complement(488..1120)
                     /locus_tag="VFV87_14830"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX5105091.1"
                     /translation="MRNSPLANRWALACWLLTAGAAGGSSGQEYAPPSRGELVAYPVR
                     QAAAEVPPRAVTRTPHKLAPRGSSQTHSPAQRATGSPSAAVGTVVSSLAIVLGLLLAI
                     VWCSRRFAPAGSAPLPKEAVSLLGRSPLGGRQTMQLVRVGSRLLLVAVSPSGAQTLTE
                     ITDPVEIEQLAAVCQRGRPDSASASFNRVLNQLASEPIEPASRTRTRGVQ"
     gene            complement(1249..1881)
                     /gene="fliN"
                     /locus_tag="VFV87_14835"
     CDS             complement(1249..1881)
                     /gene="fliN"
                     /locus_tag="VFV87_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX5105092.1"
                     /translation="MAGETPARQDDIEELLRQAQMAAGLAPEIAPAPASPPAPPSPAQ
                     DEPTAEAAQRAAARPVAATATATMPPLSTPIGPPPGGPVGDDIQYLLAQAEQALASVD
                     QPADPALEKLTPFEFKDLAASPPSGEKATLELLRDVDLNLRIELGRTQMYLEDVLKLK
                     RGSVVTLDKLAGDPVDVFVNGRIVARGEVLVLNDNFCVRVTELLAGNDFD"
     gene            complement(1935..2459)
                     /locus_tag="VFV87_14840"
     CDS             complement(1935..2459)
                     /locus_tag="VFV87_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX5105093.1"
                     /translation="MAKPILVCTFVALVILTECLFAWFLIPSTTDVQAWAKTQAQQTA
                     HGDSHAGGQTSKQGEASHGGSAENHEVEAELGRYNVVIHQPAANLTMRVNFHLIGTLP
                     EAEEAEFNALLAKNEHRLRDQVIFEIRNSRVDDLTDPGLALLKRKILAKSNELLGKPI
                     LRTVLFSDFSFIEQ"
     gene            complement(2652..>3495)
                     /locus_tag="VFV87_14845"
     CDS             complement(2652..>3495)
                     /locus_tag="VFV87_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX5105094.1"
                     /translation="QIRIVSNNGTGNEVEIDSSAFRLSAATGTITQPSLSYTSLQAAV
                     GESAVADFLAYDTLGVPVNVRVTAVLEQLTDTTSVYRWFAESGDNSPITGNDITVGTG
                     LITFDGEGKLVSVSNDDVTVERRNFPSLDPLTFQLNFGETTGLAAPRSELSAARQDGS
                     PPGTLNSYMIGEDGIIRGVFTSGIARDLGQIRLARFTNPSGLIQRGENMFATGLNSGL
                     PIEGNPNAVGLGSIIGGAVELSNTDIGKNLIDLVLASTQYRANARVISTAQQLFDELL
                     NLRR"
BASE COUNT          572 a         1136 c         1174 g          613 t
ORIGIN      
        1 cgggccatga actccttcag cggctgggcg cctgcattga acgcctcctc gggatcggtg
       61 atttcgccgc gcgagtaggg gcggatgctc tgcgaataga tttcgttcca ggtcggcgtc
      121 atgacgacca gggtcatgaa catcgccagg gcggtgatga cctggctggg tggaagctgc
      181 tgcgtgccca gcgcctgcct gagcaaaccc aacacgacaa tgatccgcac aaagctggtg
      241 gtcatcagca gcacggccgg cgccaggctc agcacggtga gcagcagcat cacctggagc
      301 gtcgagctga gccgctgcgg gctggtccat gactctgggc cgccgcggat cgcttcggac
      361 acgggatcgg tcaacagcgg cggggcaggg gagggcaggg gactggacgg gccgatgggc
      421 gcagccgtct gggccagcgc ggaagcggcc gggccgatcg acagcaagca ggcgagcagg
      481 caggttttca ttgaacgcct cgcgtgcggg ttcgcgaagc cggctcgatc ggctcgctgg
      541 ccaattgatt gagcacccgg ttgaacgagg cgctggcgct atccggcctg ccgcgctggc
      601 acacagcggc cagctgttcg atctctacag ggtcggtaat ctccgtcagc gtttgcgctc
      661 cactgggcga aacggccacg agcaacaggc ggctgccaac ccgcaccagc tgcatcgtct
      721 gccggcctcc taggggcgag cggccgagga gcgacactgc ttccttgggc agcggagcgc
      781 tccccgcggg tgcaaaccgc cgagagcacc agacgatcgc caagagcagt cccagcacga
      841 tcgccaggct gctgaccacc gtgccgacgg ctgcggacgg agagcccgtg gctcgttgcg
      901 ccggcgaatg cgtctggctg cttccccgcg gcgccagctt gtgaggggtt cgcgtcacag
      961 cgcggggcgg cacttcagcc gccgcctggc gcaccggata ggcgacgagc tctccgcggc
     1021 tggggggcgc gtattcctgg ccgctgctac cgcccgcagc accggcggtc aggagccagc
     1081 acgccagcgc ccagcggttt gccagcggcg agtttcgcat tcgagtgaaa ttcctcacgt
     1141 gatcgcagcc ggccgtcgat aacgagggtg caaaaccagg cgccgattcc agcgtccagg
     1201 cgacgccacc agcggcgcga catcgcccgc ttgtttcaga acaggccgct agtcgaaatc
     1261 gtttccggcc aacagctcgg tgacgcggac gcagaagttg tcgttgagca cgagcacttc
     1321 gccgcgggcc acgatccggc cgttgacaaa cacgtccacc gggtcgccgg ccagcttgtc
     1381 gagcgtcacc acgctccccc gcttgagctt cagcacgtcc tccaggtaca tctgcgtccg
     1441 ccccagctcg atccgcaggt taagatcgac gtccctcagc agctcgagcg ttgccttctc
     1501 gcccgaaggg ggcgaggccg ccagatcctt gaactcgaac ggggtcagct tttctagcgc
     1561 ggggtcggcc ggctgatcga ccgaagccaa agcttgctcg gcctgggcga gcaggtattg
     1621 gatgtcgtcg cctaccggcc cgccgggtgg agggccgatc ggcgtggaaa gcgggggcat
     1681 cgtcgccgtg gccgtggccg ctaccgggcg tgccgccgcg cgctgcgcgg cttccgcggt
     1741 tggttcgtcc tgcgcgggcg atggcggagc cggaggcgag gccggcgcgg gcgcaatctc
     1801 cggcgccagg ccggccgcca tctgcgcctg ccgcagcagc tcttcgatat cgtcctggcg
     1861 ggcaggcgtt tcgccggcca tcggattcct ttccactgtc ggctcgggga tccttccccg
     1921 atcggcgcgg gcagctactg ctcgatgaac gagaagtcgc tgaacaaaac cgtccgcagg
     1981 atcggcttgc ccagcagctc gttacttttc gccaatatct tccgtttgag caaggccaat
     2041 cccggatcgg tcaggtcatc gacacggctg ttgcggatct cgaaaatcac ctggtcccgc
     2101 aggcggtgtt cgttctttgc tagcagagcg ttgaactccg cttcctccgc ctcgggcagc
     2161 gtgccgatca ggtgaaaatt cacccgcatc gtaagattgg cggccggctg gtgaatgacg
     2221 acgttatagc ggcccagctc ggcttcgact tcatggttct cggcggaacc gccgtgcgaa
     2281 gcctcaccct gcttgctcgt ctggccgcca gcatgggagt caccgtgtgc agtttgctgg
     2341 gcctgcgttt tggcccaggc ctgcacgtcg gtcgtgctgg gaatcaggaa ccaggcaaac
     2401 aggcactcgg tcagaatcac gagggcgaca aaggtgcaaa caaggatcgg cttggccatc
     2461 gcgggctccc gagagagggg ccttccctgg ccggtgaacg tgccctgcgc gaggcattgc
     2521 gaccgcctcc gggccgcagg gccacgtcaa ccataggctt ttctgccggc cgaatcggcg
     2581 ttgccgttgc cgggaatcga ttcggccgac tctgcggttt gtagcgaagc aatcgccgcc
     2641 ttagcgccct tttaccgccg caggttgagc agctcgtcga acagctgctg ggccgtcgaa
     2701 atgacccggg cgttggcccg atactgcgtg ctggccagca ccaggtcaat caggttcttg
     2761 ccgatgtcgg tattcgacag ctcgaccgcc ccgccaatga tcgagcccaa tcccaccgcg
     2821 ttgggattcc cctcgatcgg caggccggaa ttgagccccg tggcgaacat gttttcgccc
     2881 cgctggatca ggccgctggg attggtgaac cgggccaggc gaatctgccc caggtcgcgg
     2941 gcgatgccgc tggtgaagac gccgcgaatg atcccgtcct cgccgatcat gtagctgttc
     3001 agcgtgccgg ggggcgatcc gtcctgccgg gcggccgaaa gctcgctccg cggagcggcc
     3061 aggccggtcg tttcgccgaa attgagctgg aacgtcagcg gatcgagcga ggggaaattc
     3121 cgccgttcca ccgtcacgtc gtcattgctc acggagacca gttttccttc gccgtcgaag
     3181 gtgatcagcc ccgtgccgac ggtaatgtcg ttgcccgtga tcgggctgtt gtcgcccgac
     3241 tcggcgaacc agcgatagac gctggtggtg tcggtaagct gctccagcac ggccgtcacc
     3301 cgcacgttga ccggcacccc gagcgtgtcg taggccagga agtcggccac ggcgctttcg
     3361 cccacggcgg cctgcagcga cgtatagctg aggctgggct gcgtgattgt gccggtcgcg
     3421 gcactcaggc ggaaggccga ggaatcgatt tcgacctcat tgcccgtgcc gttgttcgag
     3481 acgatgcgaa tctgg
//
LOCUS       DASUGB010000773         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_640156, whole genome shotgun sequence.
ACCESSION   DASUGB010000773 DASUGB010000000
VERSION     DASUGB010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640156"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFV87_14850"
     CDS             complement(<1..458)
                     /locus_tag="VFV87_14850"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5105095.1"
                     /translation="MSQPPGQSRLLALIGPGIVVAATGVGAGDLATGAFAGSKLGVAV
                     LWAVILGAAFKFLVNEGLARWQLATGKTLLEGTAAHLGRWAIWLFLAYLILWSYFVAS
                     ALIGACGAAMHALLPWQDAAKDKIVYGILHSALAVVLVRLGGFKLFEWLM"
     gene            716..1096
                     /locus_tag="VFV87_14855"
     CDS             716..1096
                     /locus_tag="VFV87_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105096.1"
                     /translation="MASDFGGILPVLADRGVQFIIVGGVAGIAHGAARATYDLDVVYG
                     RSRENLQRIVTALGPYDPYPRGAPEGLPFLWDEQTLSFGVNFTLRTTLGFVDLLGEVT
                     GGGTYEDLLPFTIELEVFGLASGA"
     gene            complement(1200..>2207)
                     /locus_tag="VFV87_14860"
     CDS             complement(1200..>2207)
                     /locus_tag="VFV87_14860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5105097.1"
                     /translation="SGAAAALDEYSSFLTSGQMDEVFSQIEGNFVGLGVELKTEPEGL
                     LIVSVIQNGPAAQSGIVPGDRLVEVDGKTTADCTPDELADMLRGAEGSLVEIVAKNAQ
                     GQLRQIRLNRRRVEVPSVEDVKIVDADNGIGYFKLTSFQKTTSRDVDAALWKLHGQGL
                     RYLIIDLRGNPGGLLKAAVDVADKFVYEGLIVATRGRSPREDFDHRGEQAGTWRVPLV
                     LLIDGDSASASEILAGAIRDHKRGTVVGVKSYGKGSVQGIFPLAAANVGIRLTTAKWY
                     TPSGQAISGTGIAPDVVVQNVHVAAKPATNELPVADGADQILQAGLQVARSQFAARQT
                     P"
BASE COUNT          404 a          709 c          694 g          400 t
ORIGIN      
        1 ctcatcagcc actcgaagag cttgaacccg cccagccgca cgagcactac ggccagagcg
       61 ctgtggagga tgccatagac gatcttgtct ttcgcggcgt cttgccacgg caagagcgcg
      121 tgcatcgccg cgccgcaggc gccgatcagc gcggaggcga caaagtagct ccagaggatc
      181 aggtacgcca ggaagagcca gatcgcccag cggccaaggt gggcagccgt gccttccagc
      241 agcgtttttc ccgtcgcgag ctgccagcgg gctaggcctt cgttgacgag aaacttgaag
      301 gccgctccga gaatcaccgc ccagagtacc gcgacgccca gcttgctgcc ggcgaacgcc
      361 ccggtcgcca ggtcgccagc gccgacgccc gtcgcagcga caacgatgcc cggcccaatc
      421 aaggccaaaa gacggctctg acccggcggc tgggacatgt tgcaagcctt tcatcgacgc
      481 gggccggccg gtagcgcata attcagtgca tgttctacga tttgcctccg cccgacgcgc
      541 ccgatccggt gatcgaattc tacaagaagg acgtgggaca gcacgctgct gcgcgagaat
      601 ctcaagctga cgccggagga gcggctgcgg cggtttttcg acttcatgcg aactctggac
      661 gagatacgcg aggcgggccg cgaacaacgg gaatcaggag gcgaggcggc tgaacatggc
      721 gagtgatttc ggcggaatac tgccggtgct ggccgaccgc ggagttcaat tcatcatcgt
      781 gggaggcgtg gcaggcattg cccacggcgc agcccgagca acatacgatc tggacgtcgt
      841 ctatggccga agtcgcgaaa atctgcagcg catcgtcacc gcgttggggc cgtatgatcc
      901 ctacccgcgc ggcgcgcccg aaggtctccc ctttctgtgg gatgaacaga ccctgtcatt
      961 tggcgtgaac tttacgctgc gcaccacact cggattcgtg gacctgctcg gcgaagtgac
     1021 gggcggcgga acctatgaag atttgctgcc ctttacgatc gagctggaag tgttcgggct
     1081 cgcgtccggt gcgtaacact cgagcgattg atccaattga agcgcgccgc gggacggcca
     1141 aaggatttgg aggccattgc ggagctgcag acgttatacg acgagcggcg tcggcccgcc
     1201 taaggcgtct gccgggctgc gaactgcgag cgggccactt gcaggccggc ctggagaatc
     1261 tgatccgcgc cgtccgcaac cgggagctcg ttcgtcgcag gtttggcagc gacatggacg
     1321 ttttgcacga cgacatcggg cgcgattccc gtgccgctaa tcgcctggcc gctcggcgtg
     1381 taccatttgg ccgtcgtcag gcgaatgcca acgttcgccg ccgcgagcgg aaaaatgccc
     1441 tggaccgagc ctttgccgta gctcttgacg ccaacaaccg tccctcgctt gtggtcgcgg
     1501 atggccccgg ccaggatctc gctggcgctg gccgaatcgc cgtcgatcaa tagcacaagt
     1561 ggaactcgcc acgtccccgc ctgttcgccg cgatgatcga agtcttcgcg cgggctgcgg
     1621 ccgcgcgtcg ccacgatcag tccctcgtac acgaatttgt ccgccacatc gactgccgct
     1681 ttgagcaatc cgccgggatt gccgcgaaga tcgataatca ggtaccgcag gccctggccg
     1741 tgcagcttcc acagggccgc gtccacgtcg cggctggtcg tcttttgaaa gctggtgagc
     1801 ttaaagtacc cgatgccgtt gtccgcatcg acgattttga cgtcctcgac gctaggcact
     1861 tcgacgcggc ggcgattcag cctgatctgc cgcaactggc cctgcgcgtt cttggcgaca
     1921 atttccacca ggctgccttc ggcgccgcgg agcatgtcgg ccaattcatc gggagtgcag
     1981 tcggccgttg tcttgccatc gacctcgacc agacggtctc ccggcacgat gccgctctgg
     2041 gcggcgggac cgttctgaat cacgctcacg atgagcagtc cctctggctc ggtcttgagc
     2101 tcgacgccga ggccgacaaa gttcccctcg atttgagaga agacctcgtc catctggccg
     2161 ctggtcagga agctcgaata ctcgtcgaga gcagcagccg cgccgct
//
LOCUS       DASUGB010000774         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1057756, whole genome shotgun sequence.
ACCESSION   DASUGB010000774 DASUGB010000000
VERSION     DASUGB010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057756"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2100)
                     /locus_tag="VFV87_14865"
     CDS             complement(<1..2100)
                     /locus_tag="VFV87_14865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105098.1"
                     /translation="MATLSLVESAEAFIETIERSGLISAETMAKVRQAAAKADSAMVL
                     AKALVKDGTLTRWQASQLLYGISQLTVGKFKLLDQLGSGEMGRVYLAEHAQMGRRHSL
                     KVLSRRHTSKPEVVKRFLDEAQQVAALEHPNVSRIYDVNQDGERYYVVMEYVEGQDLQ
                     RLVEKSGKLPVARAVEYVRQTAEGLAHAHEHQLVHGDLKPSNLLIDSSGTVKILDIGQ
                     ARLAEDEGSAEAKDETVEAAAMASAIHHAPEQRGSARKIDRQSDIYSLGSVLCNLLTG
                     KAAANAADAKMLLSRDSQIPAQLVKLCLQMMADKPADRPASMREVLQDLSAASGAASG
                     APDAPAKNGSAAAAIGSKDTEAVKSRESGTKPKKPPVARALKESAALSVAKTTEVQED
                     AASEATEAAAAEEEASPFAGFAIQTKGRKGVTKAAVKSAAAEPPAAVTEGGAPATVSA
                     KKSYLPLIIGGAIGGGMLAIGGLGLIVFLLFFRGGDSPQVADASAANDAAPAASAAKA
                     AESNPEANPESNPAAESNPTVEANPTVETSATSQARPEPTTVAVGAANTKPTPEEPAA
                     PPPGTPAAESKPAEETKPAEPPAPAPAPEPEPKPAPAPPKPAPKPAAPADPFVGFAKA
                     VTLPELGGTSGDVPPGALEPKVLGPCQIDARALVICRLKGGEFAARTGRQKFERAAAA
                     GGTAPQDWEVSLVSGGDS"
     gene            2744..3985
                     /locus_tag="VFV87_14870"
     CDS             2744..3985
                     /locus_tag="VFV87_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105099.1"
                     /translation="MAPVGYAPTADAYGTYGAVSPDMLGGDMGSGGDMSYGGEMGGGG
                     GCPYCGGTGCDMCSSGHGHGLCNGLLGDVFGLVAPYGDGGCGAVRWFDVAVDGVFLTR
                     DNRTADIVLSTFGQAGVNGIALTTGQLGFDFEGGFRFTGQIQVGPGSDLEFTYLGPLH
                     YHDQITVERLSPDPTDGLFSVFSAFGTVPPGGFVETDNADLHSVSYSSTLDSFELNFR
                     QRYMAPNCRYQGSWLWGARFVSLNETLAFLSLASQGLPPGDPPRSLNALTDTNNSLTG
                     LQIGGDYWVCLLPGLRLGAEAKAGVFYNHMNVDSVFIVNTQPLDGVNNPFFEEQIKGD
                     IAFVGEVNLQATYRVSYSWTLRGGVQGIFIDGVALAPDNFNPVPPAGPFGPGNTNRVP
                     FVSDDGNVFYYGFTAGVEYLW"
     gene            4213..>5467
                     /locus_tag="VFV87_14875"
     CDS             4213..>5467
                     /locus_tag="VFV87_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5105100.1"
                     /translation="MNGRIRKRLRGRAAPARSLGIALGLVSIVVFLARPLTAQEAEWI
                     WSPEQQKEGIPTGQACFFRQSITLRSPEAAQISIAADDRYELYVNAHKVGDGESTRKL
                     DEYDLARFLNRGVNIVAVKVVNESGNTAALAARLMVKERGANWRSYSTGPAWKASTST
                     LPLWNTALYNDRGWGEAQSFGKLAATVPWDRREDVPAAEVSRSERFTIDPQFEVQQVL
                     AGDDTGSLIAMTFNEFGHILASKEGGGLLLIYDANGDRIPEKVRIYCTKVENIQGILA
                     LNGEVFVTGEGPDGPGLYRLSDRDRDGVLENVRTLIKFNCSVAEHGAHGLVLGPDGLI
                     YVVLGNHAQLDGQYENGSPYRHYYEGDLLTPRYEDPGGHAQGIKAPGGAVIRTDTEGS
                     GVQIVAGGLRNPYDLAFTREGDLFIH"
BASE COUNT          903 a         1812 c         1697 g         1055 t
ORIGIN      
        1 cgaatcgcct ccgctcacga gcgaaacctc ccaatcctgc ggcgctgttc cgccggcggc
       61 ggcagcccgt tcgaacttct gccggccggt gcgggcggcg aattcgcccc ccttcagccg
      121 gcagatgacc agggctcgcg catcgatctg gcaggggccc agcaccttgg gctccagcgc
      181 gccgggcggg acgtcgccag aggtgccacc aagctccggc agcgtcaccg ccttggcaaa
      241 gccaacgaac ggatcggcgg gtgcagccgg cttgggagca ggtttgggcg gcgcgggcgc
      301 gggcttcggt tccggctccg gagcaggcgc cggcgcgggc ggctcggcgg gcttggtttc
      361 ctcagcaggt ttggattctg cggcaggagt gccgggcggc ggagcagccg gttcttcggg
      421 cgtcggcttt gtgttggctg caccaaccgc gaccgtggtc ggctcaggcc tggcctggga
      481 agtcgcgctc gtttcgacgg ttggattggc ctccaccgtc ggatttgact ccgcagccgg
      541 attgctctcc ggattggcct cgggattcga ttcggcggct ttcgcggcgc ttgcggctgg
      601 ggctgcatca ttcgcggcgg aggcatccgc gacctgcggc gaatcgccgc cgcggaagaa
      661 cagcaggaag acgatcagcc caaggccgcc gatcgccagc ataccgccgc cgatcgctcc
      721 gccgatgatc agtggcagat aggacttctt ggcgctgacg gtggcaggcg cgcccccctc
      781 ggtcacggcc gcgggcggtt cggcggcggc gctcttcacg gccgccttgg tgacaccctt
      841 tcggcccttc gtctgaattg caaagcccgc gaagggcgaa gcttcttcct ccgcggcggc
      901 agcttccgtc gcttccgagg cggcgtcttc ctgaacttcc gtcgtcttgg caacgctcag
      961 cgctgctgac tctttcaagg ccctggccac gggcggcttt ttcggctttg tcccggattc
     1021 ccgggattta acggcctccg tgtccttgct gccgatcgcc gcggccgctg agccgttctt
     1081 ggcgggggca tccggcgcac cggacgccgc gccacttgcg gcagacaaat cctgcaaaac
     1141 ttcccgcatc gatgccggcc tgtccgccgg cttgtcggcc atcatctgca ggcacagctt
     1201 cacgagctgg gcgggaatct gcgaatcccg gctgaggagc atcttcgcat cggcggcatt
     1261 cgcggcggcc ttgcccgtca gcagattgca cagcacgctg ccgagcgaat aaatatcgct
     1321 ttgccggtcg attttgcggg ccgagccgcg ctgctcgggc gcatggtgaa tggcggaggc
     1381 catggcggcg gcttcgaccg tctcgtcttt cgcctccgcc gacccttcgt cttccgccag
     1441 gcgcgcctgg ccaatatcca gaatcttgac cgtccccgac gaatcgatca gcaggttcga
     1501 cggcttcagg tcgccgtgta cgagctggtg ctcgtgggcg tgggcgaggc cctcagccgt
     1561 ctgccgcacg tattcgaccg ccctggccac cggcagcttg cccgactttt ccaccagccg
     1621 ctgcagatcc tggccttcga catattccat caccacgtaa taccgctcgc cgtcctggtt
     1681 cacgtcgtag atgcggctga cgttcgggtg ctccagggca gccacctgct gggcttcgtc
     1741 caggaatcgc ttgacgactt cgggcttgga ggtgtgccgc cgcgacagca ccttcagcga
     1801 gtgccggcgg cccatttggg cgtgctcggc cagatacacg cggcccattt cgcccgaccc
     1861 gagttggtcg agcagcttga atttgcccac cgtcaattgc gagatgccat agagaagctg
     1921 gctggcttgc cacctggtga gcgtcccgtc cttgaccagc gccttggcga ggaccatcgc
     1981 gctgtctgcc ttggcagcgg cctggcggac cttggccatt gtctcagccg atatcaaccc
     2041 gcttcgttcg atcgtctcga tgaaagcctc tgcgctctcc acgagcgata gcgttgccat
     2101 gattcccccg cagcgttccg ttctttgtgt gccgcaaaat gcctgtccga tggaccagtc
     2161 aagccctgaa accgcgcatc ccacgccagc agatccgcgg tgcggccgct aacgccaatt
     2221 ccccaacttt cgtaattttg attctggcaa tccgagcagc cgttggcaac tattttcgct
     2281 ggattccccg ggagcccacc aatgagccgc cgacgggaat gccagccagc gaggcagggg
     2341 aatggtcaac ttggtgaatt tgcgagaagg ctgccatttc cgaaaatcct gcggtttata
     2401 tcgaaagtac tgattttaac cgttatgacg cgccgatagc tcttgtcgag cgaaacagcg
     2461 ccagtggctc gcgggcggtc cgtgtttgcg gacgggcctc tgatcgccag ccggcgccgg
     2521 gcaaacgaca attctggagc gcggagaacc ttcgatgaat ctgcgatggg cctggacttt
     2581 gcccgtgctg tcatccttgg cagtgacgct ggccgcttcg ccgtcactgg ctcagggacc
     2641 ctatggaccg acgccggcag gttacccggc acccgcctat ggtcctgcga cgggatctcc
     2701 gtacgccccc cccggaatgc ttcccggtgg aatgcccccc gggatggcgc cggtcggata
     2761 cgcgcccacc gcggacgcct atggaaccta cggcgcggtc tcgcccgaca tgctgggcgg
     2821 cgacatgggc tctggcggcg acatgagcta tggcggcgaa atgggtggtg gcggcggatg
     2881 cccgtattgc ggcggcacgg gctgcgatat gtgcagcagc ggccacggac acggcctgtg
     2941 caatggcctg ctgggcgacg tttttggcct cgtggcgccc tatggcgacg gtggctgcgg
     3001 ggcggtccgc tggttcgatg tcgctgtcga tggcgtgttc ctcacacgag acaaccggac
     3061 cgccgacatc gtgctcagca cgtttggcca ggcaggcgtc aacggcatcg ctctgaccac
     3121 cggacaactg ggtttcgatt ttgagggcgg cttccggttc accggacaga ttcaagtcgg
     3181 accaggcagc gatcttgaat tcacctacct cggtcccttg cactatcacg atcagatcac
     3241 cgtcgaacgg ctgagccccg atccgacgga cgggctgttt tcggtcttca gcgccttcgg
     3301 cacagtcccg cctggcggat tcgtggaaac cgacaatgca gacctgcatt ccgtttccta
     3361 cagctcgacg ctggacagct tcgaattgaa cttccgtcag cgctatatgg cccccaattg
     3421 ccgctatcag ggctcgtggc tgtggggagc gcgcttcgtc tcgctcaatg agacactcgc
     3481 attcctgtct ctggcctcgc aaggcttgcc gcccggcgat ccgccgcgtt cgctcaacgc
     3541 cctcaccgac accaacaaca gcctcaccgg cctgcagatc ggcggcgact attgggtctg
     3601 tttgctgccg ggcctgcgat tgggcgcgga agccaaggcc ggcgtgttct acaaccacat
     3661 gaacgtcgat tcggtgttca tcgtcaacac acagcccctc gacggtgtca acaatccgtt
     3721 cttcgaagag cagatcaagg gcgatattgc cttcgtcggc gaggtcaatc ttcaggcgac
     3781 ttaccgcgtc agttacagtt ggacccttcg tggcggcgtg caaggcatct ttatcgacgg
     3841 cgtggccctg gcgccggaca acttcaaccc cgtgccgccc gccggtccct tcggtcccgg
     3901 aaacaccaat cgagtgccgt tcgtaagtga cgacggcaac gtgttctact acggcttcac
     3961 cgccggtgtc gaatacttgt ggtagcggct ggtcgctggt cacttgtcat tcgtcatttg
     4021 tttccgccgc aaatgacaaa tgacaggcga ccaatgacac tgctagcaag cccgatttcg
     4081 ccattgccct agccgccaca gctttgctat tttccgcggc aagtatgttt cgcgcgtcgc
     4141 catttgcggc ttcgcgcatc cttgcgcggc caaggcaggc cgccgcgtgg ccccttgggg
     4201 ccagaggtcg tcatgaacgg aaggatccgg aaacgactca ggggacgtgc ggcgccggct
     4261 cgcagcttgg gaatcgctct ggggttggtt tcgatcgtgg tcttccttgc caggccgctt
     4321 acggcgcagg aagctgagtg gatctggtcc cccgagcagc agaaagaagg catccccacc
     4381 ggccaggcct gctttttccg ccaatcgatc acgcttcgtt cgcccgaagc ggcgcagatt
     4441 tctatcgccg ccgatgaccg ctacgagttg tatgtaaacg cccataaagt gggcgacggc
     4501 gaatcgaccc gcaagctcga cgaatacgat ctcgcccgct ttctcaatcg cggcgtcaat
     4561 atcgtcgcgg tgaaggtcgt caacgaatcg ggcaacaccg ccgccctggc cgcccggttg
     4621 atggtcaaag agcgcggcgc gaattggcgc tcctattcca ccggccccgc gtggaaagcc
     4681 agcacgagca cgctgccgct ctggaatacc gcactttaca acgaccgcgg ctggggcgag
     4741 gcccagtcgt tcggcaagct ggcggccacc gtcccctggg accgccgcga ggacgtgccc
     4801 gccgccgaag tcagccgcag cgagcggttc acgatcgacc cgcagttcga ggtccagcag
     4861 gttctcgccg gcgacgacac cgggtcgctg atcgcgatga ccttcaacga atttggccac
     4921 atcctggcct cgaaggaagg cggcggcctg cttttgatct atgacgccaa cggcgacagg
     4981 attccggaaa aggtccgcat ttactgcacg aaggtcgaga acatccaggg aattctggcc
     5041 ctcaacggcg aggtgtttgt cacgggcgaa ggccccgacg gcccgggcct ctaccgtctc
     5101 tccgaccgcg accgggacgg tgtgctggaa aacgtccgca cgctcatcaa attcaactgc
     5161 agcgtggccg aacacggcgc ccacggcctc gtcctcggcc ctgacgggct gatctacgtc
     5221 gtgctcggca atcacgccca gctcgatggt cagtacgaaa acggcagccc ctatcgccac
     5281 tactacgagg gcgacctgct cacgccgcgc tacgaggacc cgggcggaca tgcccaggga
     5341 atcaaagcgc ccggcggcgc cgtcatccgc accgataccg aaggctccgg cgtgcagatc
     5401 gtggccggcg gcctgcgcaa tccgtacgac ctcgcattca cccgcgaagg cgacctgttc
     5461 atccacg
//
LOCUS       DASUGB010000775         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1057880, whole genome shotgun sequence.
ACCESSION   DASUGB010000775 DASUGB010000000
VERSION     DASUGB010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057880"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFV87_14880"
     CDS             <1..952
                     /locus_tag="VFV87_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5105101.1"
                     /translation="ADSATSATAAAIDAHLKAILAGTLPSGVDDLRAMQTHVQKLTDQ
                     LQKCTVGVTVGHAQGSGVIISKDGYVLTAAHVAGKANQPVTFNLSDGRELRGKTLGLN
                     RSIDAGLMKIEEAGEYPFAEMGASETLKEGQWCLATGHPGGYQDDRGVVLRLGRVLFV
                     GGQPQAITTDCTLVGGDSGGPLFDMDGRVIGINSRIANALSANMHVPVSTYKDTWDRL
                     AKGDAWGHFPGQTPSLGVRGEEGSPDAKIAFVYPRSAAESGGIQVGDIVIKFNDQDIA
                     DFQALKDAVDQHEPGQEVTLQVKRGEELKDITLNLGRKRE"
     gene            1070..2110
                     /locus_tag="VFV87_14885"
     CDS             1070..2110
                     /locus_tag="VFV87_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5105102.1"
                     /translation="MHRRLLSACAAAAIALGCGLLLADADRLDPKLLASFRDVVAQPA
                     KSTVQVYCDGYRSALGTIVREDGYIVTKASELKGKIQCQLQARPEKLEATLVGTDATL
                     DLAVLKIGAKNLPPIAWNEQVLPVGSWLATPGMEQDPLSIGILSVQARKIAAPAGALG
                     IRLGGNENAAQIEAIEPGMAADKAGLKANDIVLKVNGKSIGSPRQLGETIRSYMPGEK
                     VELLIKRGKDELTIHATLGSFSQLVHGDRAEFQNNLGGPLSERRGGFPLAIQHDSVLR
                     PADCGGPIVDLDGKAVGLNIARAGRVESYALPASLVREAVDKLLQTHLTSAPAGDKPA
                     QEPPPASSPSER"
     gene            2126..2398
                     /locus_tag="VFV87_14890"
     CDS             2126..2398
                     /locus_tag="VFV87_14890"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HEX5105103.1"
                     /translation="MPDRHEKLRATLHELEAELRELDSLDDETRQLLAEAALEITTAL
                     TKGEKSEQTQQVEGSLRERLEEFEASHPQLAMIVGRLIDGLGQLGI"
     gene            complement(2411..3859)
                     /locus_tag="VFV87_14895"
     CDS             complement(2411..3859)
                     /locus_tag="VFV87_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105104.1"
                     /translation="MTTETTHRMSVSDSDEAPSKPLVKRWWFVPAMAAGVLLAMFVLA
                     IVAIILQMWLSHHAAMKEVEAEVARIHSRGEPITAEDFYAWHKVPAGTPDVTQPWLQA
                     LASFDEEQLGQAGIKLPFIGEGDAALLDADAPGSQLAAAGQFLKDHERTLQLIHEAAA
                     LEGQCRFPIQFENGIATPLPHAQKMRTVQRLLQLEARARLQRGDVDGALESITHMLAA
                     GRSLQHQPTLIEQLVSIACLSMAMSEAEHLLNEAQLSDTQLASLQQSIAVQDFQGGLT
                     SGMIGERGVGYNAFQHIHSLADINALNGNGEGEMNAGGTVGRPADCLMYLQLMGEMVA
                     ASREPFPQARDRVALAEQKLKAIAGSSNPLERLKYVVTALMTPATSSAFGATARTLAR
                     RDALVTAIAAERFRLKSGSFPARLADLAPDFLPAVPTDPFDGKPLRMLVSGEEFVVYS
                     VSENGMDDQGVDNENSGKPDIVIRMRAVKQAP"
BASE COUNT          734 a         1209 c         1247 g          683 t
ORIGIN      
        1 tgcagattcg gccacctcgg ccacggcggc ggcaatcgac gcccatctga aggcgatcct
       61 ggccggcacc cttcccagcg gtgtagacga tctgcgggcc atgcagaccc acgtccaaaa
      121 gttgaccgac caactgcaga agtgcaccgt cggcgtcacc gtcggccatg ctcaggggag
      181 cggggtaatt attagtaagg acggttatgt gctgacagcc gcccacgtcg ccggtaaagc
      241 aaatcagccc gtgacgttca atttgtccga cggccgcgag ttgcgcggca agacgctcgg
      301 tctcaaccgc agcatcgatg ccggactaat gaagatcgaa gaggccggcg agtatccgtt
      361 tgccgaaatg ggcgcgtcgg agacgctgaa ggaaggccag tggtgcctgg cgaccggaca
      421 tccgggcggg taccaggacg atcgcggcgt ggtcttgcga ttgggccgcg tgctgttcgt
      481 cggcggccag ccgcaggcga tcacgaccga ttgcacgctc gtcggcggcg attcgggcgg
      541 cccgctgttc gatatggacg gtcgcgtgat cggcatcaac agccgcattg ccaatgcctt
      601 aagcgccaac atgcacgtgc cggtgagcac gtataaagat acgtgggacc gactggccaa
      661 gggggatgcc tgggggcatt ttccaggaca aactccttcg ctcggcgtcc gcggcgagga
      721 gggttcgcct gatgcaaaaa tcgcgttcgt ctatccccgc tccgctgccg aaagcggcgg
      781 gatccaggtc ggggacattg tgatcaagtt caacgaccag gacattgccg attttcaggc
      841 cctgaaagat gcggtcgatc aacacgaacc aggccaggaa gtcacgctgc aagtcaagcg
      901 tggcgaggag ctcaaggaca tcacgctgaa cctgggaaga aagcgcgagt agctgccggg
      961 cttgttgtcg gtcgatgcgg gcagttcgac ccggactccc gcgttctggc gaacgcggct
     1021 acctgtcgcg cacccgggga aaatctgttt cacgtttgga ggatctgcaa tgcatcggcg
     1081 attactttcc gcctgcgcgg ctgcggccat cgcattgggt tgtgggctac tgctcgcaga
     1141 cgccgatcgc ctcgatccga agctgctggc cagtttccgc gatgtcgtcg cccagcccgc
     1201 gaagagtacc gtccaggtct attgcgacgg ctatcgctcg gcgctgggga cgatcgtgcg
     1261 cgaggacggg tacatcgtca ccaaggccag cgagctaaag gggaaaatcc agtgccagtt
     1321 gcaggcccgg ccagagaagc ttgaggccac actcgtgggg accgatgcga ccctcgatct
     1381 ggccgttctg aagatcggcg cgaaaaacct gccgccaatc gcatggaacg aacaggtgct
     1441 gccggtcgga agctggctgg ccacgccggg catggagcag gatccgctgt cgatcggcat
     1501 tctgagcgtg caggcccgca agatcgccgc tcctgcggga gcgctcggca ttcggctggg
     1561 gggaaatgaa aatgccgcgc agatcgaagc catcgaacct ggaatggccg ccgacaaagc
     1621 gggcctgaag gccaacgaca ttgtgctgaa agtaaatggc aagtccattg gaagcccgcg
     1681 acagctcggc gagacgattc gctcgtacat gcccggcgag aaggtcgagc tgctcatcaa
     1741 acgcggcaag gatgagctga ccatccatgc cacgctgggc agcttcagcc agcttgtaca
     1801 cggtgatcgg gccgagtttc agaacaatct gggcggaccc ttgagtgagc gccgcggcgg
     1861 atttccgctg gcgatccagc acgattcggt gctgcggccg gccgattgcg gcggaccgat
     1921 cgtcgatctc gacggcaaag cggtcgggct gaacatcgcc cgcgccggcc gcgtggaaag
     1981 ctacgccctg ccggcttcgc ttgtccgcga agcggtcgat aagctgctcc agacgcacct
     2041 cacgtccgct cccgccggcg acaagcccgc ccaggagccg ccgcccgcct cttcaccctc
     2101 tgaacggtag tgcggtagaa ctggcatgcc cgatcgtcac gaaaagctcc gcgcgacgct
     2161 gcacgaactg gaagcggagc tgcgcgagct cgattcgctc gacgacgaga cgcggcaatt
     2221 gctggccgaa gcggctttgg agatcaccac cgccttgacg aaaggtgaga agagcgaaca
     2281 gacgcaacag gttgaaggct cgctccgcga gcgcctcgag gaattcgaag cctcgcaccc
     2341 gcagctcgcc atgatcgtcg gccgactgat cgatggcctg gggcaactgg ggatttgaga
     2401 tttcgcgacg ctatggcgcc tgttttacgg cacgcatacg gatcacgata tccggtttgc
     2461 cgctgttctc gttatccact ccctgatcgt ccatgccgtt ctcgctaacg ctgtacacta
     2521 cgaactcttc gccgctgaca agcatccgca gcggcttgcc gtcgaaggga tcggtcggga
     2581 cggcgggcag aaagtcgggg gccaggtcgg cgagccgcgc gggaaaggag ccggacttca
     2641 ggcggaaccg ctcggcagcg atcgcggtga ccaaggcatc gcggcgggcg agtgtgcggg
     2701 cggtcgcacc gaacgcactg ctggtggccg gcgtcatcaa ggccgtgacc acatacttga
     2761 gccgctccag cggattgctg gaaccggcga ttgccttcag cttctgctcg gccagggcga
     2821 cgcggtcgcg cgcctgtgga aacggctcgc gcgacgcggc gaccatctcg cccatgagct
     2881 gcaggtacat caggcaatca gccggccgac cgaccgtccc tccggcattc atttccccct
     2941 caccgttgcc gtttagcgcg ttgatatcgg ccagcgagtg gatgtgctgg aacgcgttat
     3001 agcccacccc tcgctcgcca atcatgccgc tcgtcaggcc gccctggaaa tcctgcactg
     3061 caatcgactg ctgcagcgaa gcgagctgcg tgtccgaaag ctgcgcttcg ttgagcaggt
     3121 gctctgcttc gctcatcgcc atcgacaggc aggcgatgct gacaagctgc tcgattagtg
     3181 ttggctggtg ctgtaacgac cggccggcgg cgagcatgtg ggtaatcgac tccagcgccc
     3241 cgtccacgtc gccccgctga agacgcgccc gcgcctcgag ttgcagcaat cgctgcacgg
     3301 tccgcatctt ttgcgcgtgc ggtaacggcg tggcgatgcc attctcgaac tggattggaa
     3361 aacggcactg cccttcgaga gccgcggcct cgtggatcag ctgcagcgtc cgctcgtggt
     3421 ccttcaggaa ctgcccggct gcggcgagtt ggctgccggg ggcatccgcg tcgagcaagg
     3481 cggcgtcccc ctcgccgatg aagggcaact ttatgccggc ctggccaagc tgttcttcgt
     3541 cgaacgatgc cagggcctgc agccaaggct gcgtgacgtc gggcgtacca gccggcactt
     3601 tgtgccaggc gtagaagtct tcggctgtga tcggctcgcc ccgcgagtga attcgggcga
     3661 cctccgcctc gacctccttc attgcggcat ggtgactcag ccacatctgg aggatgatcg
     3721 cgacaattgc cagaacgaac atcgccagca gcactcccgc cgccattgcg ggcacaaacc
     3781 accagcgctt caccaggggc ttggaaggcg cttcgtcact atcgctgacg gacatccggt
     3841 gagtcgtttc ggtcgtcatg atcatcacct acg
//
LOCUS       DASUGB010000776         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1890922, whole genome shotgun sequence.
ACCESSION   DASUGB010000776 DASUGB010000000
VERSION     DASUGB010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890922"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFV87_14900"
     CDS             <1..250
                     /locus_tag="VFV87_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105105.1"
                     /translation="AAFQTIGFSLADSGACGPGVEKVALYQRGGEYTHAARQLPTGKW
                     TSKLGKFVDIEHDATDDVGGGVYGEVFAYLKRPMQSGS"
     gene            258..974
                     /locus_tag="VFV87_14905"
     CDS             258..974
                     /locus_tag="VFV87_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5105106.1"
                     /translation="MKLSLMITCLGDVIRPSAGQAVVGVLRRLGHAIDFPEAQTCCGQ
                     PMFNSGYAELAREQARHTIRVFAGEQPVIVPSGSCAAMVKVEYPHLLEGDEVWHPKAL
                     DLSRRTYEFSDFLVNQLQVVDVGAKYAGMVAYHFACHLRMLGHTNEVERLIQHVAGAT
                     YVPLNRQDQCCGFGGSFAIRYPHISGNMVDDKLRCILATGADCVVSTDTGCMMNIGGR
                     MHREGHEVELLHIAELLQKR"
     gene            1093..2061
                     /locus_tag="VFV87_14910"
     CDS             1093..2061
                     /locus_tag="VFV87_14910"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5105107.1"
                     /translation="MPQAPTKVASPIHPFRRAILRGLAVILPPLLTVLVFLWAWGMID
                     TYILTPSERIAGQLIVWSVRDIKSGVPDGADVYRSEPGGPITAFDFRGSKYVPVGRTD
                     KYIPESVQAIVDANPFENKPSPQTAGGYYEYYVQLRYLRRSRTVPLFLALFLIVLYLT
                     GKFMAAGVGRFVWRVGEGIVHRMPIVRNVYSAVKQITDYFFSEHEVQFLRVVAVEWPR
                     KGLWTIAFVTGESFRDIKDAAGEPILSLLVPTSPMPATGFTCTVLKAETIELDITVDQ
                     AVQFIVSCGVVVPPHQQWASKKEAEEDIRRQVLGIAGTDEEMEQSA"
     gene            2190..2912
                     /locus_tag="VFV87_14915"
     CDS             2190..2912
                     /locus_tag="VFV87_14915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5105108.1"
                     /translation="MTRVVLMAAPLCFVLLLVAGCGEMPAAASKTPKKASAVAVRPLP
                     EPPKVEQDAYASVDAAMADVESVASAPPEEANAKLLKVETWLNMQGARVAPELEARIK
                     DPSVGLATRLTACRVLARLGPISVPTLLEAADGEPNQLRRKAIECLGRVKPTRSEVVA
                     KLVALTHDDDYEIRKGAFRALTSIGPPAKEHDPQLVALLTSILNDVNEDETVRSLAHD
                     ALKKIDPRIGLMGAEKGRMAGD"
     gene            complement(2974..3870)
                     /locus_tag="VFV87_14920"
     CDS             complement(2974..3870)
                     /locus_tag="VFV87_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5105109.1"
                     /translation="MATVEKYLKPEVINQIKRLDLRAQFVVKGFLQGLHASPFHGFSV
                     EFSEHRKYTPGDDPKDIDWLVYAKTDKYYVKKFEAETNITGYLAMDLSQSMGFTHRQE
                     LTKFDYAVCLAASLCYLMVYQQDPVGLVTFDTQLRDVIPPKSKRTQLGTILAHLTNLK
                     PTGPTDVAKSITQLAALLRHKSLVMIFSDLLQEPGPVFQAMRRLRHRGNDVILFHILD
                     EAEVSFPYDGMIEFEDPETLEKIVVDATSYRNDYKTEIDAFRAMYKQECFQTGVDYVP
                     LDTSMQFDKALLEYLVSRSARG"
     gene            complement(3883..>4575)
                     /locus_tag="VFV87_14925"
     CDS             complement(3883..>4575)
                     /locus_tag="VFV87_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5105110.1"
                     /translation="EGPVFSNILLADEINRTPPKTQAALLQAMQEREVTVGQTTYTLP
                     DPFFVIATQNPIEQEGTYPLPEAQLDRFMFNIKVGYPTSDEEEKILMQTTRTEKVEVR
                     KILSSRAIVNLQKLVFSVAVSEYIVKYVSRLVRATRPKDETAPQFVKDLVDWGAGPRA
                     GQNLINGGKALAAMEGRFSVAVDDIKRIAVPVLRHRLSANFQAQAEGMDTEAIILRLL
                     KEVKEPEVPKFA"
BASE COUNT          837 a         1395 c         1439 g          904 t
ORIGIN      
        1 ggctgccttt caaacgatcg ggttttctct ggcagacagc ggagcctgcg gacctggtgt
       61 cgagaaagtg gccctctatc agcgcggcgg agaatacacc cacgccgccc ggcagttgcc
      121 gaccggcaag tggacgagca agctgggcaa attcgtcgat atcgaacacg acgcgacgga
      181 cgacgtcggc ggaggtgttt atggcgaagt gttcgcttac ctcaaacgtc cgatgcaatc
      241 aggttcctga caatcgcgtg aagctttccc tcatgatcac ctgcctgggc gacgtcattc
      301 gcccgagcgc cggccaggcg gtggttgggg tgctgcgccg gctggggcat gcgatcgact
      361 ttccggaggc tcagacctgt tgcggccagc cgatgttcaa cagcggctat gcggaactgg
      421 cccgcgaaca ggccaggcac acgatccgcg tgtttgcggg cgagcagccg gtgattgtgc
      481 ccagcggctc gtgcgcggcg atggtgaagg tggagtatcc gcatctcctg gaaggcgatg
      541 aggtttggca tcccaaggcg ctcgacctgt cgcggcggac gtacgagttc agcgattttc
      601 tcgttaacca gctccaggtc gtcgatgtcg gcgcgaaata cgccggcatg gtcgcgtatc
      661 acttcgcctg ccatctgcgg atgctcgggc acacgaacga agtcgagcgg ttgattcagc
      721 acgttgcggg tgcgacctac gtgccgctca accgccagga ccagtgctgc ggctttggtg
      781 ggtcgttcgc gatccgctac ccgcacattt ccggaaacat ggtcgacgac aagctgcggt
      841 gcatcctggc gaccggggcc gattgcgttg tcagcaccga caccggttgc atgatgaaca
      901 tcggcggccg aatgcaccgc gaagggcacg aggtcgagct cttgcacatc gccgagctcc
      961 tgcaaaagcg gtaacacggg ccgcgcggcc tgtcctctcg ttacaggcgt cacatccagc
     1021 cgtcaggcgg gggcgcagca gcagccaaga tgtagaatcg ctagttggaa tcccacccgg
     1081 agccgtcccg ccatgccgca agcccccacc aaggtcgctt ccccgattca ccccttccgc
     1141 cgcgcgatcc tccgcggcct ggcggtgatt ctgcccccgc ttctgaccgt gctcgtcttt
     1201 ctctgggcgt ggggaatgat cgacacctac atcttgaccc ccagcgagcg aattgccgga
     1261 cagcttatcg tctggtcggt ccgcgacatc aaatcgggcg tgcccgacgg cgccgatgtc
     1321 tatcgcagcg agccaggcgg tccgattacc gcgttcgatt tccgcggcag caagtacgtc
     1381 ccggtcggtc gcaccgacaa gtacattccg gaatcggtcc aggcgatcgt cgatgccaac
     1441 ccgtttgaga ataaaccgtc gccgcaaacg gccggcggct attacgaata ctatgtgcag
     1501 ttgcggtatc tccgccgcag ccgcacggta ccgctgtttc tggccctgtt cctgatcgtc
     1561 ttgtacctga cgggcaaatt catggcggct ggcgtgggcc ggttcgtatg gcgcgtgggc
     1621 gaagggatcg tccaccgcat gccgatcgtc cgcaacgtct attccgccgt gaaacagatt
     1681 acggactact ttttcagcga gcacgaggtg cagttcctgc gcgtcgtggc cgtcgaatgg
     1741 ccgcggaagg ggctgtggac gatcgccttc gtcaccggcg agagctttcg cgatatcaaa
     1801 gatgccgccg gcgagccgat cctcagcttg ctggtgccca cgtcgccgat gccggcgacc
     1861 ggctttacgt gtacggtgct caaggccgag acaattgagc tcgatatcac ggtcgatcag
     1921 gcggttcagt tcatcgtgag ctgcggcgtc gtcgtgccgc cccaccagca atgggcaagc
     1981 aagaaagaag ccgaggaaga catccgtcgc caggtgctgg gaatcgccgg gacggatgag
     2041 gaaatggaac agtcggcgta gtcgttcgtc cgttcagcca tgccactgct tgcctgccgc
     2101 aggcaagcgg tgccacttgc accgggacga caacggggca caatacggcc tactggtccc
     2161 attccaattc ctctcctgcg gatgcggtca tgactcgcgt ggtacttatg gcagcgccgc
     2221 tctgcttcgt cctgttgctg gtcgccggct gcggtgagat gcccgccgca gcgagcaaaa
     2281 cgccgaaaaa ggcgtccgcc gtagccgttc gcccgctgcc cgagccgccg aaggtcgagc
     2341 aggacgctta cgccagcgtc gatgcggcga tggcggatgt agaaagcgtt gccagcgctc
     2401 ctccggagga agcgaatgcc aagctcctca aggtggagac gtggctcaac atgcaagggg
     2461 cacgcgttgc gcccgaattg gaagccagga tcaaggatcc cagtgtcgga ctggcgacac
     2521 gcctgaccgc ctgccgcgtg ctggccaggc tgggaccaat ttccgtcccc acgctcttgg
     2581 aagccgccga tggcgaaccc aaccagcttc gccgcaaggc gatcgaatgc ctcggccgcg
     2641 tcaagccgac caggagcgaa gtcgttgcga agctcgttgc gctgacacac gatgacgact
     2701 acgaaatccg caagggcgcc tttcgagccc tcacttccat tggcccgcca gctaaagaac
     2761 acgatccgca gctcgtcgca ttgctgacct ccatcttgaa cgacgtcaac gaggacgaaa
     2821 cggtccgctc gctggcccac gacgcgctca agaagatcga cccgcggata ggcctgatgg
     2881 gagcggaaaa ggggcgaatg gctggtgact agtggttgtt gctgattcac tagccactag
     2941 cactagccac cagcactccg cagaactacc gactcaaccc ctggcactgc ggctcaccag
     3001 gtattccagc agcgccttgt cgaactgcat gctcgtatcc agcggcacgt aatcgacgcc
     3061 ggtctgaaaa cactcctgct tgtacatggc ccggaaggcg tcgatttcgg tcttgtaatc
     3121 attccggtag ctggtcgcgt cgacgacgat tttttccagc gtctcggggt cttcgaactc
     3181 gatcatcccg tcgtagggga agctcacctc ggcttcgtcg aggatgtgga acaggatcac
     3241 gtcgttgccg cggtgccgca ggcggcgcat cgcctgaaag accggccccg gctcctgcaa
     3301 gaggtccgag aagatcatta cgaggctctt gtgccgcagc agggcggcga gctgcgtaat
     3361 gctcttggcg acgtcggtag ggccggtcgg cttgaggttg gtgaggtggg cgaggatcgt
     3421 gccgagctgc gtccgcttgc tcttgggcgg aattacgtcg cgaagctgcg tgtcgaacgt
     3481 caccagcccc accgggtcct gctgatagac catcaaatag cacagtgacg cggccaggca
     3541 gaccgcgtaa tcgaatttcg tcagctcctg ccggtgcgtg aagcccatcg actggctgag
     3601 gtccatcgcc aggtagccgg tgatgttcgt ttccgcctcg aacttcttga cgtagtactt
     3661 atcggtcttg gcatagacca gccagtcgat gtccttcgga tcgtcccccg gcgtgtactt
     3721 gcggtgctcg ctgaactcga ccgagaagcc gtggaatggg ctggcatgca ggccttggag
     3781 gaatcccttg acgacaaact gagcgcgaag atccaaccgt ttgatttggt tgatgacttc
     3841 gggcttgagg tatttttcga cggtggccaa ggggagccct tcctaagcaa acttcggcac
     3901 ttccggctct ttgacctcct tgagtaaccg gaggatgatg gcttcggtgt ccatgccttc
     3961 ggcctgggcc tggaagttgg cggagaggcg gtggcggagg acagggaccg cgatgcgttt
     4021 gatgtcgtcc acggcgacgc tgaagcggcc ttccatggcg gccagggcct tgccgccgtt
     4081 gatcaggttc tggccggcgc gggggccggc gccccaatcg acgaggtcct taacgaactg
     4141 cggcgccgtt tcgtcctttg gccgcgtggc gcggaccagg cgcgagacgt acttgacgat
     4201 gtactcgctg accgcgaccg agaagacgag tttttggagg ttcacgatcg cccgcgagga
     4261 gagaatcttg cggacctcca ccttctcggt ccgcgtggtc tgcatcagga tcttctcttc
     4321 ctcgtcagac gtcggatagc cgaccttgat gttgaacatg aaccggtcga gctgcgcctc
     4381 cggcagcgga taggttcctt cttgttcgat cgggttctgc gtcgcaatga cgaagaaggg
     4441 atcagggagc gtataggtcg tttggcccac cgtcacttcg cgttcctgca tcgcctgcag
     4501 cagcgccgct tgcgtcttcg gcggcgttcg gttgatttcg tccgccagca gaatgttcga
     4561 gaagaccggc ccttc
//
LOCUS       DASUGB010000777         6104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1615076, whole genome shotgun sequence.
ACCESSION   DASUGB010000777 DASUGB010000000
VERSION     DASUGB010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615076"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFV87_14930"
     CDS             <1..1104
                     /locus_tag="VFV87_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5105111.1"
                     /translation="QPPIAFSATENVAWKQPLPGRGVSGPIVIKDRVVVTASSGPVKE
                     DKLHVLCFDAESGKQLWHRQFWATGRPYHHPTSSNAAPTPASDGERIFAFYSSNDLVC
                     LDLDGNLLWYRGLAFDWPKAGNDVGMSSSPLVIGDTVVVQVENQGDSFAAGLDTATGE
                     TRWRVSRPNKANWVSPAAFRGNSGKDAVLLQSGDGLTAYDAKSGEQLWKYDVACAGIP
                     SVTAAGDRIYLPANGLTALDVAAGSTSPSLAWDSNRLSPGNASPVVLGDRVYLLAKAG
                     VLNCGDAATGDLLWQERLKGTFWATPVIANGHAYCVNQDGQCLVVNLAGEKGEVVHTA
                     EIGEGFFGTPAVAGNGLYLRGEKHLWKIAEPKP"
     gene            1126..2595
                     /locus_tag="VFV87_14935"
     CDS             1126..2595
                     /locus_tag="VFV87_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5105112.1"
                     /translation="MTSARQAISLIIALTVGASPLLGVASDERPNRPNIVLILLDNVG
                     QEWFGCYGSEEGCTPHIDALAKAGMRAEHCYTPPVCGPSRTVLLTGRYPHSTGFRLHH
                     DAALYSGGGLDPEREILFPRLFREAGYATCIAGKWQVNNLYDEPGVLTRHGFDEQLVW
                     PGSIDRTKIPAAEWERFLYIVRRQSYEEAVEFNRHIESRYWNPILLRNGKRKEAKGQF
                     GPDVCQAFAIDFVRRHANQPFLLYVPMMLTHGQDFSHPVVPTPLNRSADRPEQEMFAD
                     MLRYGDKLIGELIAELERLKLRDNTIVVVASDNGTEKHLHARRNGRVVQGDLYSLSEA
                     GGDVVLLANCPRLIPGGRIVPLTDFTDIYPTLCELAGIPLADRHKPDGQSLAPYLLGK
                     PGAKPPRSWILNEYHDTRVVRDERFKLYSDGRFFDANADPAEQHDLAASTDPAAVAAK
                     VRLQQVLGSLPPDQPSPFPLRSLSAFKLRAEAAAKAPPP"
     gene            2708..4018
                     /gene="aroA"
                     /locus_tag="VFV87_14940"
     CDS             2708..4018
                     /gene="aroA"
                     /locus_tag="VFV87_14940"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5105113.1"
                     /translation="MPAPDSIEITPVTRPVRGRIRPPGSKSITNRALVCAALAEGAST
                     LTGALDSEDTRVMIESLGRLGIRVDVRDSGQMLVVHGCGGKIPAKQADLFVGNSGTTI
                     RFLTALCALGHGTYRLDGIPRMRERPIGDLLDALNQLGADCRGELRTGFPPVIVRAAG
                     LRCPDEGIHVRGDISSQFLSAVLMAGPASETDIEIFVDGTLVSEPYIAMTLRVMEQFG
                     TFVDRLNDDYFHSEGNQRYAGRQYAIEPDASAASYFWAAAAITGGEVTVEGLAKDSLQ
                     GDVQFVECLAKMGCSVDYAADEITVSGRARWGIDVDMNAISDTVQTLAVVALFAEGPT
                     TIRNVAHIRHKETDRLGDLARELRQLGAEVNELPDGLRIVPPTEPSHLKAATLETYND
                     HRMAMSLALAGLRIPGVIITNPECTAKTYPRFFEDLNRLICGRI"
     gene            4304..4660
                     /locus_tag="VFV87_14945"
     CDS             4304..4660
                     /locus_tag="VFV87_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX5105114.1"
                     /translation="MLLGVWQQYSMKLDGEDPPAGISQVDTWEFKEDTVGRPESPFGT
                     WNFDPTRSPKHFQFQLFDKGPRLGLYELEGDHLTLCYGTDEGRRPSTIKAGYGTVLLK
                     FRRDTNFPNQASDPIR"
     gene            4724..>6104
                     /gene="ettA"
                     /locus_tag="VFV87_14950"
     CDS             4724..>6104
                     /gene="ettA"
                     /locus_tag="VFV87_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX5105115.1"
                     /translation="MSRQYIFTIERLLKKHGQREVLKDIWLAFYPGAKIGVLGRNGAG
                     KSTLLRIMAGLDKDFDGEARLTDGFTRGYLSQEPQLTPNLDVWGNLQEAIAPRRKQVD
                     RYNEISAKLAEPMDDDQMQKLCDEMAKLQDQIEATGAWEIDRELEIAMDAMNLPDKEA
                     DVATLSGGERRRVALCKLLLQKPDLMLLDEPTNHLDAEAINWLERTLADYHGTIVAVT
                     HDRYFLDNVAKWILELDRGMGIPWEGNYSSWLEQKKARLEREERQSQARQKTLERELE
                     WIRMSPRARQAKSKARISAYEKMSAERFEERPDEFEIQIPPGKHLGDLVVEAKKLTKG
                     YGDRVLIDNLDFRLPAGGIVGVIGPNGAGKTTLFRMIVGQEQPDSGELRVGPTVELGY
                     VDQNRDALEAEKTVYQEISGGNDHLEMGGKKINARAYVNRFNFRGPDQEKKVGVLSGG
                     ERNRVHLAKL"
BASE COUNT         1201 a         1970 c         1885 g         1048 t
ORIGIN      
        1 cagccgccta tcgcgtttag cgcgactgaa aacgtcgctt ggaaacagcc gctgcccggc
       61 cgcggcgtca gcgggccgat tgtgatcaag gatcgcgtgg tcgtcaccgc cagctcgggc
      121 ccggtgaagg aagacaagct gcacgtcctg tgcttcgacg cggaaagcgg caaacagctt
      181 tggcaccggc agttctgggc gacgggccgg ccgtatcacc atccgacgag ttccaacgct
      241 gctcccacgc cggccagcga cggtgagcgg attttcgcct tctattcgtc caacgatctg
      301 gtctgcctcg acctggatgg caacctgctc tggtatcgcg gcctggcctt cgactggccc
      361 aaggcaggca acgacgtcgg catgtccagc tcgccgctgg tgatcggcga tacggtggtt
      421 gtgcaggtcg agaaccaggg cgattcgttc gccgccggcc tcgacacggc caccggcgag
      481 acccgctggc gcgtcagccg gccgaacaaa gccaactggg tctctcccgc ggcttttcgc
      541 ggcaacagcg gcaaggacgc ggtgctgctg caatcgggcg acggcctgac cgcctacgac
      601 gccaaatcgg gcgagcagct ttggaaatac gatgtggcgt gtgccggcat tccctcggtc
      661 accgccgcgg gcgaccggat ttatctgccc gccaatggcc tgacggcgct cgacgtcgct
      721 gccggttcca ccagcccgtc gctggcctgg gattccaatc gcctctcgcc gggcaacgcc
      781 agcccggtcg tccttggcga tcgcgtctat ctgctggcca aggcgggcgt gctcaattgc
      841 ggcgatgccg cgacaggcga cctgctctgg caggagcggc tcaaaggcac cttctgggcg
      901 acgccggtca tcgccaatgg ccacgcttat tgcgtcaatc aagacggcca gtgcctcgtc
      961 gtcaacctcg ccggtgaaaa gggtgaagtc gtccacaccg ccgaaatagg cgaaggcttc
     1021 ttcggcactc ccgccgtcgc cggcaacggc ctctatctgc ggggcgaaaa gcatttgtgg
     1081 aagattgccg aaccaaagcc gtagctcaat agtcggaggt ctcacgtgac cagcgcacgg
     1141 caagcaattt cattgatcat tgccctgacc gttggtgcaa gtccgctcct tggggtcgcg
     1201 agcgacgaga gaccgaatcg ccccaacatc gtcctcatcc tcctcgacaa cgtcggccag
     1261 gagtggttcg gctgctatgg cagcgaggag ggttgcacgc cgcatatcga cgccttggcg
     1321 aaggcgggga tgcgggccga gcattgctat acgccgcccg tttgtggccc cagccgtacg
     1381 gtgctcctca ccggcagata tccgcacagc accggctttc gcttgcacca tgacgcggcc
     1441 ctgtatagcg gcggcgggct cgatccagag cgcgaaatcc tcttcccgcg gctctttcgc
     1501 gaggctggct atgcgacctg catcgccggc aagtggcagg tgaacaacct ctacgacgag
     1561 ccgggcgtcc tcacgcggca cggcttcgat gagcagctcg tctggccggg cagcatcgac
     1621 cggacgaaga ttcccgccgc cgagtgggag cggtttctct atatcgtccg ccgccaatcc
     1681 tacgaagaag cggtcgagtt caaccgtcat atcgaaagtc gatattggaa tccgatattg
     1741 ctgcgcaacg gcaagcggaa ggaagcgaaa ggtcaattcg gcccggatgt ttgccaggcc
     1801 ttcgccattg acttcgtccg gcggcatgcc aaccagccgt ttctgctcta tgtgccgatg
     1861 atgctcacac acgggcagga tttttcgcac ccggttgtgc caacgccgct caaccggtcc
     1921 gccgatcgcc ccgagcagga gatgttcgcc gacatgctcc gctacggcga caagctcatc
     1981 ggcgaattga ttgccgagct ggagcggctg aagctccgcg acaacacgat cgtcgtcgtc
     2041 gccagcgata acggcaccga gaagcactta cacgcccgcc gaaatggccg cgtcgtgcaa
     2101 ggcgacctct attcgctcag cgaagccggc ggggatgttg tcctgctggc gaattgcccc
     2161 aggctcattc caggcgggcg aattgtgccc ctgaccgact ttaccgacat ctatcccacg
     2221 ctctgcgagc tggccggcat tccgctcgcg gatcgtcaca agcccgatgg ccaatcgctg
     2281 gctccgtatc tgctcggcaa gcccggcgca aagccgccgc gatcttggat tctcaacgaa
     2341 taccacgaca cccgcgtcgt ccgggacgag cgattcaagc tctattccga cggccgcttc
     2401 ttcgacgcca acgccgaccc cgccgagcag cacgacctgg ccgccagcac cgaccccgcc
     2461 gcagtcgccg cgaaagtccg cctccagcag gtcctcggct cccttccgcc cgaccagcca
     2521 tcgccgttcc cgctccgcag cctctcggca ttcaaactcc gggcggaagc tgccgcgaaa
     2581 gcaccgccgc cctgagcgcg cgggagcagg accgcttaga atgggcgatt gtcgtcgttc
     2641 gctccgcgaa cgaacgccct ttcgcggagc gaaaggcgac gatcccatcc tccgtcaagg
     2701 tcgcatcgtg cccgcccctg attcgatcga gatcacgccc gtcacgcgac ccgttcgcgg
     2761 ccggattcgc ccgccgggct ccaaaagcat caccaaccgg gccctcgttt gcgcggccct
     2821 ggcagagggc gccagcacgc tgaccggtgc gctcgacagc gaagatacgc gggtgatgat
     2881 cgaaagcctc ggccgcctcg gcattcgcgt ggacgtgcgg gattccggcc agatgctcgt
     2941 cgtccacggc tgcggcggga aaatcccggc gaaacaggct gacctgtttg tcggcaacag
     3001 cggtacgacg atccggtttt tgacggccct gtgtgccctc ggccacggca cgtaccgcct
     3061 ggacggcatc ccgcggatgc gcgagcggcc gatcggcgac ctgctggacg cactcaacca
     3121 gctcggcgcc gattgccgcg gcgagttgcg caccggcttt ccgccggtga tcgtccgggc
     3181 agcaggattg cggtgtccgg atgagggaat tcacgtacgc ggagacatat cgagccagtt
     3241 cttgagcgcc gtgctcatgg ccggcccggc gtccgaaacc gacatcgaga tctttgtgga
     3301 cggaacgctt gtctctgaac cgtacattgc gatgacactt cgtgtcatgg agcagtttgg
     3361 cacgttcgtg gacaggttga atgacgatta ctttcacagc gaaggaaatc aacgctacgc
     3421 cggcaggcag tacgccatcg agcccgacgc ttccgccgcc agctatttct gggccgcggc
     3481 ggcgattacc ggcggcgaag tgacggtcga ggggctggcc aaggactcgc tgcaagggga
     3541 cgtgcagttc gtcgaatgcc tggcgaagat gggctgctcg gtcgattacg cagccgacga
     3601 gatcacggtt tctgggcgcg cccgctgggg catcgacgtc gacatgaacg cgatcagcga
     3661 cacggtgcag acgctggccg tcgtcgctct ctttgccgaa ggccccacca cgatccgcaa
     3721 cgtcgcccac atccgccaca aagaaaccga tcgcctcggc gacctcgccc gcgagctccg
     3781 ccagctcggc gcagaggtca atgaactgcc cgacggccta agaattgtgc ctcccaccga
     3841 accgtctcac ctcaaggcgg ccaccctcga aacgtacaac gaccaccgca tggccatgag
     3901 cctcgccctc gccggcctcc gcattccggg cgtcatcatc accaaccccg aatgcacggc
     3961 gaagacgtat ccgaggtttt tcgaagacct gaatcgcctc atttgtggaa gaatctgatt
     4021 ccacgttcga tggactcaac aagacttggg gaacacttgc atgtatccgt acaatcaatc
     4081 aacaccacgt agcgcgacag tgatttggat gactccgtca ttcggttggg cctggatcct
     4141 gaccgtactt acattgtccg gatgccagga ccatgcccag ccggcaggag gtccagccgc
     4201 gaaggctgct caacaacctg cagctccgcc accaccgcct ggcgtcgacg tgcagcccgc
     4261 accagtcgaa gcagggtcag acgcaatcgc cgcagagagg gcgatgcttt tgggagtctg
     4321 gcagcagtat tctatgaagc tcgacgggga agatccgccc gcgggaatca gccaggtcga
     4381 tacctgggaa ttcaaggagg atactgtggg ccggccggaa tcgccctttg gtacttggaa
     4441 tttcgatccg accaggtcgc cgaagcattt ccagttccag ctcttcgaca agggaccgcg
     4501 cctgggactg tatgaactcg aaggcgacca tctgaccctg tgctacggca ctgacgaagg
     4561 tcgccggccc agcacaatca aggccggcta cggaaccgtg ctgctcaagt ttcggcgcga
     4621 taccaacttt ccgaaccagg cttccgaccc cattcggtaa ttgaatgagg ggggttaaat
     4681 tgatggctgg ctatttgaaa actcttcgtc tccccggcca gccatgtccc gccaatacat
     4741 cttcacgatc gaacgcctcc tcaaaaagca cggccagcgg gaggtgctga aggatatctg
     4801 gctggcgttt tatccggggg ccaagatcgg ggtgctgggc cgcaatgggg cgggcaagag
     4861 cacgctcttg cggatcatgg cgggcctcga caaggacttc gacggcgagg cccgcctgac
     4921 cgatggcttt acgcggggat atctctcgca ggagccgcag ctcacgccga acctggatgt
     4981 gtggggaaac ttgcaggagg cgatcgcccc gcgacggaag caggtcgatc gctacaacga
     5041 gatttcggcc aagctggccg agccgatgga cgacgatcag atgcagaagc tctgcgacga
     5101 gatggccaag ctgcaggacc agatcgaggc gaccggggcc tgggaaatcg atcgcgagct
     5161 ggaaatcgca atggatgcga tgaatctgcc cgacaaggaa gccgacgtcg cgaccctttc
     5221 gggcggcgag cggcggcgag tcgccctgtg caagctcttg ctgcaaaagc ccgacctgat
     5281 gctcctcgac gagccgacga accacctcga cgccgaggcg attaactggc tggagcggac
     5341 gctggccgac tatcacggca cgatcgttgc cgtcacgcac gaccgctatt ttctggacaa
     5401 cgtcgccaag tggattctgg aactcgaccg cgggatgggc atcccgtggg aaggcaacta
     5461 ttcctcctgg ctcgagcaga aaaaggcccg cctggagcgg gaagaaaggc aaagccaggc
     5521 ccggcagaag actttggagc gcgagctgga gtggattcgg atgtcgcccc gcgcccgcca
     5581 ggccaaaagc aaggcgcgca tcagcgccta tgaaaagatg tccgccgagc ggttcgaaga
     5641 gcggcccgac gagttcgaga tccagattcc accgggaaaa cacctgggcg acctggtcgt
     5701 ggaagcgaag aagctgacca agggctatgg cgaccgcgtg ctgatcgata acctcgattt
     5761 ccgcctgccc gcgggcggga tcgtgggcgt gatcggcccc aacggggcgg gaaagacgac
     5821 gctcttccgg atgatcgtcg gtcaggaaca gcccgacagc ggcgaattgc gggtcggtcc
     5881 gaccgtggaa ctgggctacg tcgatcagaa ccgcgacgcg ctggaagccg agaagaccgt
     5941 ctatcaagaa atatccggcg gcaacgacca tctggagatg gggggcaaga agatcaacgc
     6001 ccgggcttac gtgaaccgct tcaacttccg cggcccggat caggagaaaa aggttggggt
     6061 gctttcaggc ggcgaacgca accgcgtcca cctcgcgaag ctcc
//
LOCUS       DASUGB010000778         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_780620, whole genome shotgun sequence.
ACCESSION   DASUGB010000778 DASUGB010000000
VERSION     DASUGB010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780620"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4226)
                     /locus_tag="VFV87_14955"
     CDS             complement(<1..>4226)
                     /locus_tag="VFV87_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5105116.1"
                     /translation="GLVATIDNPPFERLGTQIGPYKLLEQIGEGGFGVVYMAEQASPV
                     RRRVALKLIKPGMDSHQVVARFEAERQALAVMDHPHIAKVLDAGTTDSGRPYFVMELV
                     RGVPVTQYCDENSLSVRERLDLFMAICQAIQHAHTKGIIHRDIKPTNVLVTRQDGQPV
                     VKVIDFGIAKAMGQRLTERTLFTGFAQMIGTPLYMSPEQAELSSTDIDTRSDIYSLGV
                     LLYELLTGSTPVNKKQLKEAAFDEIRRIIREDEPPKPSTRISSAESAPSIAAQRHTEP
                     AKLARLVRGELDWIVMKALEKDRNRRYETASGFARDIERYLNDEPVLACPPSAAYRFG
                     KFARRNRGALAIASVAVLSVLLALVGLTVSNVLITQEKNLKVDALEKVKLNEKAANTQ
                     KGIAERNATKAREQEQKATVNEKKAKFSELLARRRYYAAQMNLAMQAWEAGQAARVLA
                     LLESQRPKIDEEDLRTFEWYYLWRLCQGGLRHRFPTVNHDNSAVLALSPDGLTLASGF
                     GNDVKLWDTLTGQEKKTLSGHSTMVNQLAFTSSGILISSDNVTFRIWNPATAAEQLVP
                     IAGSGNGGDRFAISGDGKILVIDLPAGITLCDLDTGQQSVVEGSAGTNRFGIVAVDAG
                     VQRVAACRDNSLIRVWERDGANWRELPSIPEHGWCPCLAFSPDGKSLAVSGTLRCYDP
                     ATGKLQLTPLGHTGGVLSVGYSADGKTLVSTGHDQTARLWDAATGRQLACLAHPVMVY
                     GAALSADGKVAATAGDGVRVWDTTPLDETVILAHTAAVLAVALSRDGKTLASAGNDGT
                     KLWNLPERKEVKSFPPSKDVAFFPDDKALALFRTSGTIELFNITGERQAELKGHTSAR
                     SLAVSPDGKCLASACYEPEAILWDLHTRQQRSKLKMSGQLPEVAFSRDGQILAAGSQF
                     GAVKLFEAATGRETATLQRFEFASTFITGLAFSRDSALVAAANGEGQVQVWEIATGRL
                     HAALRGHASTVAGLSFSPDGQTLATASADHTVKLWDVVTGQERFTLKGHKGPVTAVEF
                     MPDGNTLITASEDGTVRLWLAAVDSQAKARKQELDWDVPGTPAACHERGDAFWQYEHL
                     DQAEIAYAEADARLQKLAVAFPESAELKREMVRSLLTRSLLLDETGRTQKAGPLRDRA
                     HGIYGNLTANDQQVLIWEFCERGRKLATMGNHRQAERTYAQAIDLEPTNERARSLRRS
                     TFFAMGDWDKVVVDSSQLIELNPNEAGNWNWRGVAHSRQGQKETALADYSRAIQLNPL
                     EPIFWKNRGTCYSELGEHDKAITDYSKALELSSEDASTLNRRALAYDKLGQNEQAQAD
                     WKAALELLKKSLAKQPGDDSADWFALAIVHWRLGNKNPARNSFEQAVLRMGQSQQPTE
                     WLLQIRSETAALLELTDPPVAIASQPPMSA"
BASE COUNT          756 a         1284 c         1286 g          900 t
ORIGIN      
        1 cggccgacat aggaggctga gaagcgatcg cgacgggtgg atccgtcagc tccagaagtg
       61 cggcggtttc gctgcgaatt tggagcagcc attccgtcgg ctgttgactc tgtcccatcc
      121 gcagcacggc ttgttcaaac gaattgcgcg ccgggttctt gttgccaagt cgccaatgga
      181 cgattgcgag agcgaaccaa tcggcgctgt catctccagg ctgcttcgca agcgactttt
      241 tcaggagctc taaggcggct ttccagtcgg cttgcgcttg ctcgttttgc ccaagcttgt
      301 cgtaggccaa ggcccgccgg ttcaaagtgc tcgcgtcttc cgaactcaac tcaagagctt
      361 ttgagtaatc cgtgatggcc ttgtcatgct cgcccaactc gctgtaacag gtgcctcggt
      421 ttttccagaa tatcggctcc agcggattca attgaatggc ccgcgagtag tccgccaggg
      481 ccgtttcctt ttgcccttgc cgactgtggg cgacgccgcg ccagttccaa ttaccagctt
      541 cgttcggatt cagctcgatg agctgtgagg agtccacgac aactttgtcc caatcgccca
      601 ttgcgaagaa ggtactgcgc cgcaaagagc gggctcgctc gtttgtcggt tcaagatcaa
      661 ttgcctgggc ataggttcgt tctgcttgcc ggtggttgcc catggtcgcc agctttctgc
      721 cgcgctcgca gaattcccaa atcaaaacct gttggtcatt ggccgtgagg ttgccgtaga
      781 tcccatgagc ccgatcgcgc agcgggcctg ctttctgcgt ccgaccggtc tcgtcgagaa
      841 gcagacttcg cgtgagcaag ctgcgaacca tctcccgctt gagttcggca gactctggga
      901 aggcgacggc gagcttctgg agtcgggcgt cggcttcggc gtaggcgatt tcggcctggt
      961 cgagatgctc gtattgccag aacgcatcac cccgctcatg gcaggccgcg ggagttccgg
     1021 gaacgtccca atccaattct tgcttgcgcg ctttggcctg ggagtccacc gcggcgagcc
     1081 agagccggac tgtcccgtct tcactagcgg tgatcagagt gttcccatcg ggcatgaact
     1141 cgactgcggt gactggtcct ttgtgcccct tgagggtgaa acgttcctga cctgtgacga
     1201 cgtcccacaa cttgaccgtg tgatcggcac tggctgtcgc cagcgtctgg ccgtcaggcg
     1261 agaaactgag ccccgcaaca gtgctggcat gtccccgcag cgccgcgtgc aggcggccgg
     1321 tggcgatctc ccagacttgt acctggccct ctccgttggc ggcggcaacg agagcgctat
     1381 cgcgtgaaaa ggccagtcct gtgataaacg tcgaggcgaa ttcaaaccgc tggagcgtgg
     1441 cagtctcgcg cccggttgca gcctcgaaga gctttaccgc gccgaactgg gagccggctg
     1501 ccaggatttg gccgtcgcgc gagaaggcga cttccggcaa ctgcccggac atcttgagct
     1561 tgctgcgctg ctggcgggtg tggagatccc agagaatcgc ctccggctcg taacaggccg
     1621 aggccaggca tttgccgtcg ggggagactg ccagactgcg cgcgctagtg tgacctttga
     1681 gctcggcttg gcgctcgccc gtgatgttaa aaagttcgat ggttcccgaa gtgcggaaca
     1741 gcgccagggc cttgtcatca ggaaaaaatg caacatcctt cgatggcggg aaactcttta
     1801 cctccttgcg ttccggcaag ttccacagct tggtgccatc gttccccgcg gatgcaagtg
     1861 tcttgccatc gcgcgagagg gccacggcca acacggcggc cgtatgcgca agaatgaccg
     1921 tttcgtccag cggcgtagta tcccagactc ggactccgtc gccggcggta gcggctacct
     1981 tgccgtcagc cgatagggcg gcgccataca ccatcaccgg atgagccaga caggcgagtt
     2041 gtcggcccgt tgctgcgtcc cacaaccggg ctgtctgatc gtggcccgtt gacacgagcg
     2101 tcttgccatc ggccgagtag ccaaccgaca aaacgccgcc cgtgtgcccc aagggagtaa
     2161 gctggagttt gccggtcgcg ggatcgtagc acctgagcgt gccggaaacg gccagcgatt
     2221 tcccatcagg cgagaaagcc aggcaaggac accagccgtg ttccggaatc gacggcagtt
     2281 cccgccaatt cgctccgtcc cgctcccaga cgcgaatgag gctgttgtcg cggcaggcag
     2341 ccacacgctg gacgccggca tcaaccgcca cgatgccgaa acgatttgtt ccggcgctgc
     2401 cttcgacgac cgattgttgg ccggtatcta ggtcgcaaag ggttatgcca gcggggaggt
     2461 cgatgacgag tattttgcca tcgccggaga tagcaaagcg gtcgccaccg tttccagagc
     2521 cggcaatcgg caccaattgc tccgctgcgg tcgccgggtt ccaaatgcgg aacgtgacat
     2581 tgtcgcttga aatcaagatc ccgctggatg taaacgccag ttgattcacc atggtgctat
     2641 ggccacttag cgtcttcttt tcctgcccgg tgagcgtgtc ccaaagcttg acatcattgc
     2701 caaagccgga ggccagggtc agtccgtcgg gagaaagagc caggaccgcc gaattgtcgt
     2761 ggttcacagt cggaaaacgg tgccggagtc cgccctgaca gaggcgccac aggtaatacc
     2821 attcaaaggt gcgcaaatcc tcttcatcga tctttggtcg ctggctttcg agcagtgcca
     2881 gcacgcgagc tgcttgcccg gcctcccagg cctgcatggc cagattcatc tgggcagcgt
     2941 agtagcggcg gcgggcgagc agctcactga acttcgcttt cttctcgttg acggtggctt
     3001 tctgttcttg ctcccgggct ttggttgcat tgcgctcggc aattcccttc tgcgtgttgg
     3061 ctgccttttc gtttagcttg accttttcca gggcgtcgac cttgaggttc ttttcttgtg
     3121 tgattaacac gttgctgact gtcaggccga ccagcgccag cagcaccgac aacacagcga
     3181 cgctagcgat cgccagtgcg cctcgatttc gccgggcaaa ttttccaaat cgatatgccg
     3241 ccgaaggcgg gcaggccaac acgggctcgt cgttgaggta ccgttcaata tcgcgagcaa
     3301 acccgctagc cgtctcgtag cggcgattac ggtccttctc cagcgccttc atcacgatcc
     3361 agtccagttc gccacgcacg agcctagcca gcttggcggg ctcggtgtgg cgctgcgcgg
     3421 cgatgctggg cgcggactcc gcggaactga tccgcgtgct tggtttgggc ggctcgtcct
     3481 cacgaatgat cctgcggatt tcgtcaaacg ccgcctcctt cagttgcttc ttgttgacag
     3541 gcgtcgaacc ggtcaaaagc tcgtacagca gcacaccgag cgaatagata tcgctccgcg
     3601 tgtcgatgtc ggtgctgctc agctcggcct gctcggggct catgtagagc ggcgtgccga
     3661 tcatctgggc gaagccagtg aagagagttc gctcggtcag acgctgcccc attgctttgg
     3721 cgatgccgaa gtcaatcact ttcactaccg gctggccgtc ctgacgcgtg accagcacat
     3781 tcgttggctt gatgtcacgg tggatgatgc ctttggtatg cgcgtgctga atggcctggc
     3841 agatcgccat gaacaagtcg agccgttccc ggacggacag gctgttttcg tcgcagtact
     3901 gcgtgaccgg aacaccgcgg accagttcca tgacgaagta aggccggccc gagtcggtag
     3961 ttccggcatc cagcaccttc gcgatgtggg gatggtccat gaccgccagg gcctgccgct
     4021 ccgcttcgaa gcgggcgacc acttggtggg agtccatccc gggtttgatt agcttgaggg
     4081 ccacgcggcg acgcacgggg gacgcctgct cggccatata gacaacgccg aatccccctt
     4141 cgccgatctg ctccaggagc ttgtaggggc cgatctgcgt gcccagccgt tcgaacggag
     4201 gattgtcgat cgtcgcgacc aggccc
//
LOCUS       DASUGB010000779         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1891303, whole genome shotgun sequence.
ACCESSION   DASUGB010000779 DASUGB010000000
VERSION     DASUGB010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891303"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VFV87_14960"
     CDS             <1..1001
                     /locus_tag="VFV87_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5105117.1"
                     /translation="SAAIAHLAIEQESVARQMEDCIARRDEALAERGRVADQLAAFRR
                     RAQKLRDQQHQLELSAEQVRHERRSLADRLREDYGIDLAAATDQPSPQEEQERAAVEE
                     EIESLRRKINQIGAVNLDALDELDDLEQRFATLSGQHKDLTEAKQALERIIHKINADS
                     RRLFLETLEAIRLNFQALYRKAFGGGKADIVLEEGVDVLDCGVDIIATPPGKPSFNNS
                     LLSGGEKALTAVALLLAIFQFRPSPFCVLDEVDAPFDEANISRFMDVLKDFLSWTRFI
                     LVTHSKKTMTAATTLYGVTMQESGVSKRVSVRFDDVTDDGHIRREALDRAAGEEAA"
     gene            1067..>2928
                     /locus_tag="VFV87_14965"
     CDS             1067..>2928
                     /locus_tag="VFV87_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5105118.1"
                     /translation="MSLDLFHPLIRQWFAGRFGEPTEPQVRGWPQIAAGRHTLIAAPT
                     GSGKTLAAFLVCLDRLLRQWLEGKLPCGVEVVYVSPLKALSNDIQRNLQSPLAEIAAL
                     AAEAGLGDLPIRTAVRTGDTPQAERAAMLRHPPHILVTTPESLYLLLTSAKSREKLRT
                     VKTVIVDEIHALARDKRGSHLSLSLERLAALCDQPPTRIGLSATQKPIDEIGRFLVGN
                     CGLRIVDCGLQAAKPDSSNPKSKIQNPKSSTTPFDVPEIVDIGHARNLDLNFELPQLE
                     LEAVCTHEHWGEVYERLEQLIQSHRSTLVFVNTRRLAERVAFRLTERLGEDAVASHHG
                     SLSREIRLDAEQRLKAGRLKAIVATASLEMGIDIGYIDLVCQIGSPRAIATFLQRVGR
                     SGHAVGALPKGRLFPLTRDELLECLALIRAVRRGILDRIEIPVAPLDILAQQIVAAVA
                     ADEWDETALFDLFRRAAPYRDLQRKQFDAIVQMLSEGIKRGGKAGAYLHRDQINGRLR
                     ARRGARLAAITSGGAIPETAQYRVVTETEGTFVGTVDEDFAIESLAGDVFLLGNSSWM
                     IRAVRGDEVIVADAHGAPPTIPFWLGEAPGRTIELSQELSDLRREVGLRIADCG"
BASE COUNT          530 a          961 c          929 g          508 t
ORIGIN      
        1 catcggcagc gattgcacac ctggcgatcg agcaggaatc ggtggcccgg caaatggagg
       61 actgcattgc ccgccgcgat gaggccctgg ccgagcgggg cagagtcgcc gatcagctgg
      121 ccgccttccg ccgccgcgcg cagaagcttc gcgaccagca gcatcagttg gaactctcgg
      181 ccgagcaggt ccgccacgag cggcggtcgc tggccgatcg cctgcgcgag gactacggca
      241 tcgacctggc cgccgccacc gatcagccga gccctcagga ggagcaggag cgggcggccg
      301 tcgaagaaga gatcgaatcg ctccgccgca agatcaacca gatcggcgcg gtcaatcttg
      361 acgccctgga cgagctcgac gacctggaac agcgcttcgc cacgctctcg ggccagcaca
      421 aggacctgac cgaggccaaa caggccctgg aacgcatcat ccacaagatc aacgccgaca
      481 gccggcggct gttcttggag acgctggagg cgatccgcct caatttccag gctctctatc
      541 gcaaggcgtt tggcggcggc aaggcggata tcgtgctcga agagggtgtc gatgtattgg
      601 attgcggcgt cgatatcatc gccactcccc ccggcaagcc gagctttaac aactcgctcc
      661 tctcaggcgg cgagaaagca ctcaccgccg tcgcgctgct cctagcgatc tttcaattcc
      721 ggccaagccc cttctgcgtg ctggacgaag tcgacgcccc gttcgatgag gcgaacatca
      781 gcaggttcat ggacgtgctg aaagactttc tctcctggac gcggttcatt ctggtgacgc
      841 atagcaagaa gacgatgacc gccgccacga ccctctatgg cgtgacgatg caagaatcgg
      901 gcgtctcgaa gcgcgtcagc gtccgcttcg acgatgtcac cgacgatggc cacatccgcc
      961 gcgaagcgct cgaccgcgcc gcgggcgaag aagcggctta gtcgctggtc accctatgcc
     1021 gattgtgtac aattagcgag tacacttttc gttttggcat ggacgtatgt ccctggacct
     1081 cttccacccc ctgatccgcc agtggttcgc cggccggttc ggcgagccga cggagccgca
     1141 ggtgcgcggc tggccgcaga ttgccgcggg ccggcatacg ctgattgccg cgccgaccgg
     1201 gtcgggcaag acgctggcgg cgtttctggt ctgtctcgat cgtctgttgc ggcagtggct
     1261 ggaggggaag ctgccctgcg gggtcgaggt ggtgtatgtc tcgccgctga aggccctgag
     1321 caatgatatt cagcggaatc tgcagtcgcc tttggcggaa atcgccgctt tggcagccga
     1381 agctgggctc ggcgatttgc cgatccgtac tgcggtccgc acgggagata cgccgcaagc
     1441 cgagcgggcg gccatgctcc gccatccgcc gcacattctg gtcacgaccc ccgaatcgct
     1501 ctacctgctg ctcaccagcg ccaagagccg cgagaaactg cgcacggtca agacagtcat
     1561 cgtcgatgag attcacgccc tggcccgcga caagcgcggc tcgcaccttt cgctctcgct
     1621 ggagcgcctc gccgccctct gcgaccagcc gcccacacgg atcggccttt cggccacgca
     1681 gaagccgatt gacgagatcg ggcgattcct ggtcggcaat tgcggattgc ggattgtgga
     1741 ttgcggatta caggcagcaa agccagattc ctccaatcca aaatccaaaa tccaaaatcc
     1801 aaaatcatct accacgccat ttgatgtccc cgagatcgtt gacatcggcc acgcccgcaa
     1861 tctcgacctg aactttgagc tgccgcagtt agagctggag gcggtctgca cccacgagca
     1921 ctggggcgag gtctatgagc ggctggagca gctcatccag tcgcaccgca gcacgctggt
     1981 cttcgtcaac acgcggcggc tggcggagcg ggtggcgttt cgcctgacgg aacgcctggg
     2041 agaggatgcc gtggccagcc atcacggcag cctgtcgcgc gaaatccgcc tcgatgcgga
     2101 gcagcgtctg aaagccggca gactgaaggc aattgtggcg acggcgtcac tggagatggg
     2161 gatcgacatc ggctatatcg atctggtctg ccagattggc tcgccaaggg cgattgcgac
     2221 cttcctgcag cgcgtcgggc gctccggcca cgcggtcggc gctttgccca aagggcggct
     2281 cttcccgctg acgcgcgacg aattgctgga atgtctcgcc ctgatccgcg ccgtccgccg
     2341 cggcatcctc gaccggatcg aaatccccgt tgctccgctc gacatcctgg cccagcaaat
     2401 cgtcgccgcc gtcgcagccg acgaatggga cgaaacggcg ctcttcgacc tcttccgccg
     2461 ggccgctcct taccgcgatc tgcagcggaa gcagttcgac gccatcgtgc agatgctctc
     2521 cgaggggatc aagcgcggcg gcaaggcggg cgcctacctg caccgcgacc agatcaacgg
     2581 ccgtctgcgg gcccgccgcg gcgcgcgact ggctgcgatc acctccggcg gggcgatacc
     2641 tgagacggcc cagtaccgtg tcgtgaccga gacggaaggg acgttcgtcg gcaccgtcga
     2701 tgaagacttc gccatcgaaa gcctggcggg cgatgtgttc ctgcttggca attcctcctg
     2761 gatgatccgc gccgtccggg gcgatgaagt gatcgtggcc gacgcccacg gcgctccgcc
     2821 caccatcccc ttctggctcg gcgaagctcc cggccgcacg atcgaactgt cgcaggagct
     2881 gtcggacctg cggcgggagg tgggattgcg gattgcggat tgcggatt
//
LOCUS       DASUGB010000780         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1891378, whole genome shotgun sequence.
ACCESSION   DASUGB010000780 DASUGB010000000
VERSION     DASUGB010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891378"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VFV87_14970"
     CDS             complement(<1..1308)
                     /locus_tag="VFV87_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105119.1"
                     /translation="MTALAVPPVAEVPRAANRARSAPGKSPDRSAKLNKVEQFKLAKD
                     GLDVLPDIARFAAADDESQIDADDGQRLKWFGLFLRKQTPGHMMMRLRATCGKMNAAQ
                     WRVIADLSDEFGKSFCDLTTRQQVQMRWFTIGQVPQIWDRMLAVGLTTLQTGMDNVRG
                     VCGCPASGITPSELFDASPAAEDFTGLFLGERAYTNLPRKFNATITGCRENCCHPETQ
                     DLGLVPALAEIGGEPTPGFNVLVGGKQGSGGLRPATPLDVFVTPSEAPAICGHITRIF
                     SDHGSREARNRARLAFLIEDRGIAWFREELERRVGRDLLPAGIDQRTKAHTDHVGIYR
                     QKQPGLNYAGLLVPVGRITTKQMRGVADLAERYGNGEIRLTTSQNVLLPGIPDGRLGD
                     FEQEPLLKELPVNPSPVMRGLVSCTGNDYCHLALIETKGWAIDV"
     gene            complement(1646..>2658)
                     /locus_tag="VFV87_14975"
     CDS             complement(1646..>2658)
                     /locus_tag="VFV87_14975"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX5105120.1"
                     /translation="LLWREARRGEVIAAHEPGSTAEFLTKRVAALPGEEWGIRGGDLY
                     ADGEIVRKSLHDLREVRVLVHDNAFQPRLAKGLPPRWQPADGNSRWKASSFGFAADGN
                     ADDSAAFDWLQYHHWRCTESSAPRAQSVPILDNDSYNQNLSRQLNPVRDVLLACTVRT
                     SGEGQLAFAAMDGSRRWEARLDLGQRQMTLLDSGQPVLEKPFTVRFDRRAIPIEFGLC
                     DGQVLLSVAGRIVVVWPYKLAKTEPPDVLDPLAIGAKSLDVQVTDLKIWRDIYYLAPF
                     GTASRWQADQPLAAGEFALLGDNPPASEDSREWPGSGGISRSEFLGVVMRPFWARSRS
                     LP"
BASE COUNT          434 a          848 c          901 g          475 t
ORIGIN      
        1 gacgtcgatc gcccagccct tcgtttcaat gagcgcgagg tggcaataat cgttgcccgt
       61 acacgacacg aggccccgca tcacgggcga cggattgacc ggcagctcct tgagcagcgg
      121 ttcttgttcg aagtcgccca gcctgccgtc gggaatgccc ggcaagagca cgttctggct
      181 cgtggtcagg cggatttcgc cattgccata ccgctctgcc aggtcggcga cgccgcgcat
      241 ctgcttcgtc gtgatccggc cgaccggcac gagcaggccg gcatagttca gccccggctg
      301 cttctggcga tagatgccga cgtggtcggt gtgggctttg gttcgctgat caatgccggc
      361 cggcagcaag tcgcggccga cgcggcgctc cagttcctcg cgaaaccagg cgatgccgcg
      421 gtcttcgatc aggaatgcca ggcgggcgcg gttgcgagcc tcgcgcgagc cgtgatcgct
      481 gaagatgcgg gtgatgtggc cgcagatcgc tggcgcttcg ctgggcgtga cgaatacgtc
      541 cagcggcgtc gcgggccgca ggccgcccga gccttgcttg ccgccgacca gcacgttgaa
      601 cccaggcgtc ggttcgccgc caatctccgc cagcgccggc acgaggccca ggtcctgcgt
      661 ttccgggtgg cagcaattct cgcgacagcc ggtgattgtg gcgttgaact tgcgcggcag
      721 gttcgtatag gcccgctcgc cgaggaacag gccggtgaag tcttcggccg cgggcgaggc
      781 gtcgaacagc tcgctgggcg tgatgcctga agccgggcag ccgcagacgc cgcggacgtt
      841 gtccattccg gtttgcagcg tcgtcaggcc gacggcgagc atccggtccc agatttgcgg
      901 gacctggccg atcgtgaacc accgcatctg gacttgctgg cgggtggtca ggtcgcagaa
      961 gctcttgccg aattcgtccg agaggtcggc gatgacccgc cactgggcgg cgttcatttt
     1021 gccgcacgtc gcccgcaggc gcatcatcat gtggccgggg gtctgcttgc ggaggaagag
     1081 gccgaaccac ttcagccgct ggccgtcgtc cgcatcgatt tggctttcgt cgtccgctgc
     1141 ggcaaagcgg gcgatatcag ggaggacgtc gagcccgtcc ttggcgagct tgaattgctc
     1201 gaccttgttg agcttggcgc tgcggtccgg cgatttgccc ggcgcggagc gggcgcgatt
     1261 ggccgcgcgc ggaacttccg ccacaggcgg cactgcaaga gccgtcatac gtaggtttct
     1321 tctcccgcga gccgccgcct gtattggcgc gccgggagtt gcgagggcga atctcaggtt
     1381 ccttgcccgc gaggccgcca gaagtcttcc gccgcgcttg cgcgcagaca ggaagtcccc
     1441 gccaggcacg cgaccgccgg ctgatgtggg agagaatcag cctggcgaat agtcgcacgg
     1501 cacagctatt acaaattgcg cgccaacgtg tgatggggac ttgctgatgc tgcttataag
     1561 gtcctttagt gactgtagtt acagcgagat cgggaatctg gtcggtgaac gacgtgcgcc
     1621 gttgggctgc aggctctgcc cagtctcaag gcaacgaacg ggagcgagcc cagaaggggc
     1681 gcatcacgac gcccaagaac tccgatctcg aaatcccgcc gctgccgggc cactcgcgac
     1741 tgtcttccga agcgggcggg ttatcgccga gcagcgcgaa ctcgcccgcg gccagcggct
     1801 ggtcggcttg ccagcggctg gcggttccaa aaggcgcgag atagtagatg tcgcgccaga
     1861 tcttgaggtc tgtgacctgg acgtcgaggc tcttggcgcc aatggccagc ggatcaagca
     1921 cgtcgggcgg ttcggttttg gccaacttgt aaggccacac gacgacgatt ctgcctgcga
     1981 cggacaacag cacctgccca tcgcacaggc caaactcgat cggaatcgcc cgccggtcga
     2041 agcgcacggt gaatggcttt tccagcacgg gctggccgct gtccaggagg gtcatttgcc
     2101 gctggccgag atccagccgc gcctcccacc gtcgcgagcc gtccatggcc gcgaaagcga
     2161 gttggccctc gccactggtc ctcacggtgc aggcgagcag cacgtcgcga accggattga
     2221 gctgccgcga gagattctgg ttgtagctgt cgttgtcgag gatgggcacg ctttgcgcgc
     2281 gcggggcgga gctttcggtg cagcgccagt ggtgatactg gagccaatcg aaggcggcgg
     2341 aatcgtctgc gtttccgtca gccgcaaacc cgaagctgct ggctttccag cgggaattgc
     2401 catccgcagg ctgccaccgc ggcggcaagc ccttcgccag ccgcggctga aaggcgttgt
     2461 cgtgcacgag gacgcggacc tcgcgcaggt cgtggagcga tttgcggacg atttcgccgt
     2521 ccgcatacag atcgccgccg cgaatccccc attcctcgcc cggcagcgcc gcgacgcgct
     2581 tggtcaggaa ttccgcagtc gaccccggct cgtgagcagc aatgacctcg ccgcggcggg
     2641 cttcccgcca caaaagct
//
LOCUS       DASUGB010000781         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_920039, whole genome shotgun sequence.
ACCESSION   DASUGB010000781 DASUGB010000000
VERSION     DASUGB010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920039"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..939
                     /locus_tag="VFV87_14980"
     CDS             184..939
                     /locus_tag="VFV87_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HEX5105121.1"
                     /translation="MPIHDWTRADAGAFHHLHLSWIAQLSAVLNQGLLPPQYYALAEA
                     RAGETEPDVLTLKLRETMSFEPESPPGPARQGPPGAIALAEAPPQVEIVTDLDTDFYV
                     RRRRTLVIRHASDHRIVALIEVVSPGNKSSQHAFDQFLEKIFSALDQGINVVLIDLHP
                     PTLRDPRGLHAVITEAYGREIAGLGAAKNLTLASYLAGSERRAFAQPVAVGDHLTPMP
                     LFLEDAYYIQLPLEETYMAAWNSIPRHIQQSLT"
     gene            977..>2243
                     /gene="glnA"
                     /locus_tag="VFV87_14985"
     CDS             977..>2243
                     /gene="glnA"
                     /locus_tag="VFV87_14985"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX5105122.1"
                     /translation="MTPREVLAFCREKEVQAVDLRFCDLLGTWQHFTIPVGRLSEGLF
                     EDGLGFDGSGIQGWQGIHESDMLVIPQADTALLDPFALVPTLVLICNVQDPVTGEPYS
                     RDPRHVAIKAANYLRHTGIADVAHFGAEIQFFVFDSARFEQNAHEGYYHLGSREGQWT
                     RGEPASGAGESPNLTYKLRHQEGYFPCPPADQLMDLRSEMMQAMQGCGLDVEAHHHEA
                     ASAGQCAIDLAYQPLVTMADRVLAYKYCVRNAARRRNQTATFMPQPLFGDQGSGMHTH
                     LSLWKDDEPIFAGHGYAGLSDVGLHAIGGILKHAPAILAFSCPTTNSYKRLVPGYEAP
                     VNLAYSQRNRSASCRIPMYSPSPKAKRIEFRCPDPSCNPYLSFATLMMAALDGIQNKI
                     HPGEPLDKDIYDLAPEELAEVPKTPGSLGE"
BASE COUNT          421 a          718 c          685 g          419 t
ORIGIN      
        1 tggccgctgc cgacctacca ggagatgctg ttcattaagt aggtgagtgg tgcgtcggtg
       61 agtaggtgag tggtgctaac cagtgcggga ggccgacggg cctcccgctg ctgttttgcg
      121 acccgtttgc cggtcggact ataattggcg gtgacagttc gctgatttta tcggagcagg
      181 cgaatgccga ttcacgactg gactcgcgct gacgcggggg cgttccacca cttgcacttg
      241 tcgtggatcg cccaactgtc ggctgtgctc aaccagggac tcctgcctcc gcagtattac
      301 gcacttgccg aagcccgtgc gggggagact gagccggacg tcctgacttt gaaactgcgg
      361 gaaaccatga gcttcgagcc ggagtcgccg ccgggacccg cgcgacaagg tccgccgggc
      421 gcgattgccc tagcggaagc gccgccgcaa gtggaaatcg tgaccgattt ggatacggac
      481 ttctatgtgc gccgccgacg aacgctggtc atccggcacg cttccgacca tcgcatcgtg
      541 gccttgatcg aggtcgtttc accgggaaac aagtccagcc agcacgcctt tgaccagttt
      601 ctggagaaga tattctctgc gctagatcaa ggcatcaacg tggtgctgat cgacttgcat
      661 cctcccaccc tgcgagatcc gcgcgggctg cacgcagtga ttacggaagc gtatggacgt
      721 gaaattgccg gactcggcgc cgcaaagaat ctgaccctcg cgtcgtatct tgctggaagc
      781 gagcgccgcg ctttcgcgca accggtagcc gtcggggatc acctaacgcc gatgccattg
      841 tttctcgagg acgcctacta cattcagctc ccgctggagg aaacgtacat ggctgcctgg
      901 aattcaattc ccagacatat ccagcaatcg ttgacttgaa gatcgacggc agcctgtcac
      961 gcccgaaaaa ctcaccatga ctcctcgcga agttcttgct ttctgccgcg aaaaagaagt
     1021 gcaggccgtc gatctgcggt tctgcgatct cctgggcacg tggcagcatt tcacgattcc
     1081 cgtcggcagg ctcagcgaag gcctgttcga ggacgggctc ggcttcgacg gctcggggat
     1141 ccagggctgg caggggattc acgagagcga catgctcgtg atcccgcagg ccgatacggc
     1201 gctgctcgat ccttttgcgc tcgtgccgac gctggtcctg atctgcaacg tgcaggaccc
     1261 cgtgaccggc gagccctatt cgcgcgatcc gcggcacgtc gcgattaagg ccgccaacta
     1321 cctgcggcac acgggcattg ctgacgtggc gcacttcggc gcggaaatcc agttcttcgt
     1381 cttcgatagc gcccgcttcg aacagaacgc ccacgaaggc tattatcacc tcggcagccg
     1441 cgaggggcag tggacccgcg gcgagccggc gagtggagcg ggcgagtcgc cgaatctcac
     1501 ttacaagctg cgacaccagg aaggctactt tccctgtccg ccggccgatc agctcatgga
     1561 cctgcgcagc gaaatgatgc aggcaatgca gggctgcggc ctggacgtgg aggcccacca
     1621 tcacgaagcc gcttccgccg gccagtgcgc catcgacctg gcctatcagc cgctggtgac
     1681 gatggccgac cgcgtgctgg cctacaagta ctgcgttcgc aacgccgccc gccgccgcaa
     1741 ccagacggcc accttcatgc cgcagccttt attcggcgac caggggagcg gcatgcacac
     1801 gcacctctcg ctgtggaagg acgacgagcc gatcttcgcc gggcatggct atgcggggct
     1861 gtcggacgtg ggcctgcacg cgatcggcgg catcctcaag cacgccccgg ccatcctggc
     1921 cttctcctgc cccacgacca acagctacaa gcggttggtg ccgggttacg aagcgccggt
     1981 caatctggcc tactcacagc ggaaccgctc ggcctcgtgc cgcattccga tgtacagccc
     2041 gagccccaag gccaagcgga tcgagttccg ctgccccgac ccgagctgca atccctacct
     2101 ctccttcgcc acgttgatga tggccgcact cgatggtatt caaaacaaga tccatccggg
     2161 cgaaccgctt gataaggaca tctatgacct ggctccggag gagctggccg aggtgccgaa
     2221 gacgccgggt tcgctgggcg aag
//
LOCUS       DASUGB010000782         6433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1474853, whole genome shotgun sequence.
ACCESSION   DASUGB010000782 DASUGB010000000
VERSION     DASUGB010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474853"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFV87_14990"
     CDS             complement(<1..426)
                     /locus_tag="VFV87_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105123.1"
                     /translation="MQVFRILCVGSILSVGTLANAVVRAKSPSPDAEPVELFAAIESG
                     EIDVKLFLKDSTGGTVTIANNTKKPLTIKLPEAFAGVPVQAQFGGGMMGGGMMGGMNG
                     GMMGGGMGGMNQGFGGGMMGGGMGMMGGGMGMGRGMGGGG"
     gene            complement(684..1184)
                     /locus_tag="VFV87_14995"
     CDS             complement(684..1184)
                     /locus_tag="VFV87_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105124.1"
                     /translation="MKSTLLFTLVCGVLLLATSAEALADGGLLPNLNPFSSQRQKKAP
                     VSSRGGAPTSGWQWPRLWPTSSSSPQRKSSSPSTWQKMNAGTKNFLSQTADTLNPFND
                     AEDNKPQPGMTGSNSIFSQASQSRKKNEEKSGFSLIPSWWGGQEEEQKDSTVNEFLMR
                     KRPGFE"
     gene            1755..2501
                     /locus_tag="VFV87_15000"
     CDS             1755..2501
                     /locus_tag="VFV87_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5105125.1"
                     /translation="MSRKPLIGLNVDYRPSRKEGPALAYLMAGYFNCIQVAGGIPVIV
                     PPSDCQDDLERILDLLQGFVLVGGGDLDPRRDGFQLHSSIRPMDPRREDFDRMLMGCI
                     ADRRLPVFAIGSGLQLLNVSQGGSLFLHLPEDLPTSIPHRDPHDPEHRHGLEVVFGTL
                     MERVYGDGEIRVNSMHHQAIDEVAPGFQVTARCPDGVIEAIESTQEDWWAIGTQFHPE
                     AESASALDVRVFEEFIDGIIKPKRAMKLVA"
     gene            complement(2940..3116)
                     /locus_tag="VFV87_15005"
     CDS             complement(2940..3116)
                     /locus_tag="VFV87_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105126.1"
                     /translation="MDQSSIEPDERRLLAVVARIGARSALRNERNDAQLLDNAQQRNM
                     PSRARVGAQLVISQ"
     gene            3487..3927
                     /locus_tag="VFV87_15010"
     CDS             3487..3927
                     /locus_tag="VFV87_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105127.1"
                     /translation="MSKAGPKSRRKSSLPKLIDKYYADLADLARQNVLFEMGTRPAFH
                     ALLAAAGKEHGWTLIAEQEKKVGGKTIRPDGTFKGEMYLVRGYWEAKDTADKLDAEIE
                     KKRKAGYPLNNIIFEEPETAVLFQHGQPILAEDMMERRTDHISR"
     gene            4027..4647
                     /locus_tag="VFV87_15015"
     CDS             4027..4647
                     /locus_tag="VFV87_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105128.1"
                     /translation="MSSSGTNSSSTASPLEAAQKIVAILDGMTNEHRLLAVKFALETL
                     GLQAPTAQVHEPQALQHPPQSVQAAATPPLRRMDIKTFTNMKAPKTDTQFTAVVAYFY
                     QIESPEDQRKESINIETMKEAARLVGGRRQPPNWKFTLQNARNAGYLEAAGDGNYRLT
                     PVGENLVAITLPGNAVAGGGNGAGKKKKSSKKAGKKATRKKAAKNR"
     gene            4685..5494
                     /locus_tag="VFV87_15020"
     CDS             4685..5494
                     /locus_tag="VFV87_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105129.1"
                     /translation="MPTVHEVLDAAVNEATAARTRVNRVRTVQVRRVDDVAMLKATAQ
                     TWFHTHRPAVAIGAPHVDLTGIDELYMKVLGATAKYTAKSTYLATLKEAKEELIAIRA
                     AALVAPRPSAVANTDDLAPDFSPLAGNQEMRDILTRRWHECAKCVKAEVHLAAIVMMG
                     GLLEALFVARANKMADKTPLTSATDAPKDKTTGKTINYQEWMLDSHIKVGFELGWITE
                     SAKGVADKLKEYRNFVHPAKELRYGVALGFNDSSMFWQVTKALARQLLLSA"
     gene            5491..6213
                     /locus_tag="VFV87_15025"
     CDS             5491..6213
                     /locus_tag="VFV87_15025"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX5105130.1"
                     /translation="MRTRCAVYGLLHHPGYRTKYPDNLKRELPRIPFAPEFRPFAKVG
                     ENLAKLHLDYEQLKPYDLDFIETPGLPLSYRVEDKMRLSKDKLRLAVNPSLTLAGIPP
                     EVFDYRLGNRSALGWVIEQYQVSTDKRSGITSDPTPWSKLAASLTRSSASAWRRSRLC
                     RGCRQSLSQSRRASFASRLHGPWHSFRVRGNDATRYPGLRLLAHPGLRLLTQACPGLS
                     CVGLSGRGVQHRRCGPPAVCRA"
BASE COUNT         1365 a         2094 c         1746 g         1228 t
ORIGIN      
        1 gccaccacct cccatccctc gccccattcc catcccgccg cccatcatcc ccatgccacc
       61 gcccatcatc ccgccgccga acccttgatt catgcccccc atgccaccgc ccatcatccc
      121 gccattcatg ccccccatca tgccgccgcc catcatcccg ccgccaaact gcgcttgaac
      181 cggcacgccg gcaaacgctt ccggcagctt gatcgtcagc ggctttttcg tgttgttggc
      241 aatcgtcacc gtgccgccgg tcgaatcctt gagaaacaac ttcacgtcga tctcgccgga
      301 ctcgatcgcg gcaaacagct ctaccggctc ggcgtccggc gaaggcgact ttgcgcgaac
      361 aaccgcattc gccaaggtcc cgaccgacag tattgaaccc acgcacagaa ttcgaaagac
      421 ttgcatgaag acaccccgct tctcgcaacg acctgctgct catcgggcac acagcaaggg
      481 cgaattgcca atactctaac cgccgtcaat tggccgattc gagcaagaaa tggggagctt
      541 acgaatttgt gtgtttcatc agcgaatgcg ctcgtcaatc agacggcagc cttacggatg
      601 cgtaaggctg cgggccaaaa acctcccgta caaagccctg ctgggcggag ctggtttggg
      661 cgaaccagtt tcgctcggga cgcctattcg aatcctggcc gtttccgcat caggaactcg
      721 ttgaccgtgc tgtctttctg ctcctcttcc tggccgcccc accacgaagg aatcaacgag
      781 aagcccgact tctcctcgtt cttctttctc gactgcgatg cctggctgaa gatgctgttc
      841 gagcccgtca tgccgggctg cggtttgttg tcttcggcat cgttgaacgg gttgagcgtg
      901 tcggccgtct ggctgaggaa gttcttggtg ccggcgttca tcttctgcca ggtcgaggga
      961 ctcgaagatt tgcgctgcgg cgacgaagaa ctcgtcggcc acaggcgcgg ccactgccag
     1021 ccgctcgtcg gcgcccctcc tcgactggaa accggcgcct tcttctgccg ctgggagctg
     1081 aatggattga ggttcggcaa caggccccca tcggccagcg cctccgccga agtcgcgagc
     1141 aacaacactc cgcagacgag cgtgaacaac aaagtcgatt tcatggcgac actccctgca
     1201 caccgcggtc acttcaggaa aaaacggggg tcacttgtcc caaatgcacg cctccgcgcc
     1261 aagagcggcg aacgacgctc gctgcacgcg gctcatttgc gtcgcaccgt atgccgatcc
     1321 atttgctagc aatcccccgc atgcacaaca ccttgtgcta gcggtcccac ccgaccacaa
     1381 aatatggggc cattcttgcg gcctcccgcg accggattac attcccgcga tctcgctggc
     1441 gcgttcctgc accagcaaga gaaggggttc acacggatag ggagatcgct tcagcggcac
     1501 gttcgggagg aacttcgccg ggagcatacg cagcaggcct tgggaaaggc ctcgtcaggg
     1561 acgaccgcga ggcggcttct tctgccgcga tcgtggccac aaccccgccg agccaccagc
     1621 cgcttcccgc aagcggctcg tctgcccgtg cgctctctcg aatcttcgcc cggcgtccgc
     1681 tgcgcctggc aagccgccag gcaacgttcc cgcaagggag ttcgggtctg ttcctcgttc
     1741 ctaagggact ttgcatgtct cgcaagccac tgattggtct gaacgtcgat taccgcccgt
     1801 cgcgcaagga ggggcctgcg ctggcgtacc tgatggcagg gtatttcaac tgcattcagg
     1861 ttgcaggggg aattccggtg atcgtgccgc cctccgattg tcaggacgac ctcgagcgga
     1921 tcctcgatct gctgcaaggc ttcgtcctgg tcggcggcgg cgatttggac ccccgccgcg
     1981 acggcttcca gctccacagc agcatccgcc cgatggatcc gcgccgcgag gatttcgacc
     2041 ggatgctcat gggctgcatc gccgatcgcc gtctgcccgt cttcgccatc ggctccggct
     2101 tgcaattgct caacgtctcg caggggggct cgctctttct gcacctgccc gaagacctgc
     2161 cgacctccat cccgcaccgc gatccgcacg atccggaaca ccgccacggt ctggaggtcg
     2221 tcttcggcac gctcatggag cgcgtctatg gcgacggcga aatccgcgtc aacagcatgc
     2281 accatcaggc cattgacgaa gtcgcccccg gcttccaggt caccgcaaga tgcccggatg
     2341 gcgtcatcga agccatcgag agcacccagg aggactggtg ggccatcggc acccagttcc
     2401 accccgaagc cgaatccgcc tcggccctcg acgtccgcgt gttcgaggaa ttcatcgacg
     2461 gcatcatcaa gcccaaacga gcgatgaagc tggtggccta gttttgccgg tggaaaatca
     2521 aggattcacg tatctacgta ggatggacct cctggtccgt cctcgtgagt cgttgtgcga
     2581 cgttctcgtt cccaggctcc agcctgggaa cgcgagtgtc ccgaggctcc tgcctcgtga
     2641 gtcgccaggc acggggttgc gcgcaacttc gtgcactttt tccgcgcaac tcctcgcgaa
     2701 gcaacgttcg taagttcttg tgttctcaat agttgcgcgc cgactctcgc gcgcaactgc
     2761 atgttgcgct gttgcgcgtt gtcaagcagt tgcgcatcct tgcacctcgt tcccaggctc
     2821 gtgcctggga gcgcgagcgt cccggaggct cctgcctcgc gaatcaccag gcgaaagggg
     2881 ttgcgcgcaa cttcgggcat tttttttcgc ccaactctcc ccgaagcggt gttcgtaagt
     2941 cattgcgaaa tcaccagttg cgcgccgact ctcgcgcgcg acggcatgtt gcgctgttgc
     3001 gcattgtcaa gcagttgcgc atcgtttctc tcatttctca aagccgagcg cgccccaatc
     3061 cgcgcaacga ccgccaacag ccggcgttca tctggctcga tgctcgactg atccatgcat
     3121 gcatatcgca cgaaaaaact caccgtcaac gtccatgatc gtaactcctt tatataaaac
     3181 aacttgcgat agaatcgccg tccaggcgct tatgcaaatt atgcaccgta tgcatcgctg
     3241 cctcccacca cgaccaagtt gccaaagacc accgccgcgc gcaccgcggc ccaatcacta
     3301 agatgcgcaa gtggacagat ttatttcatc caagcgccaa attttcacaa ttttcctctg
     3361 gaccgcgttt gcatctgtca ccaccaccgg tagattgccc atccggggga acccggctcc
     3421 tcctgccgca ctgccgggca tgagtacaaa cccgcatcgt cattccgcct gaattccgcc
     3481 tgacccatgt ccaaagccgg cccgaaatcc cgccgcaagt cctcgctccc caagctcatc
     3541 gacaagtact acgccgacct cgccgacctc gcccgccaga acgtcctctt cgaaatgggc
     3601 acccgccccg ccttccacgc cctcctcgcc gccgccggca aggagcacgg ctggaccctc
     3661 atcgccgagc aggaaaagaa ggtcggcggc aaaaccatcc gccccgatgg caccttcaag
     3721 ggcgaaatgt acctcgtccg cggctactgg gaggccaaag acaccgccga caagctcgac
     3781 gccgaaatcg agaaaaaacg caaagcaggc tacccgctca ataacatcat cttcgaggag
     3841 cccgaaacgg cggtcctctt ccaacacggc cagccaatct tggctgagga catgatggag
     3901 aggcgaaccg accacatctc caggtagtcg ttatcttgtc aatagcaatc gccccattcc
     3961 actattacaa tcgcgtttgc acaggcatca tcttgcgcac caaacgtgcg agtcagcgag
     4021 tgtgaaatgt cctctagtgg cacgaattca agcagtacgg caagcccgtt ggaagccgcg
     4081 caaaaaattg tcgcgatcct agatgggatg acgaatgaac atcgcttgtt ggccgtaaag
     4141 tttgcgttag aaacgctcgg gcttcaagcg ccgacggctc aggtgcatga gccgcaagcg
     4201 ctgcagcatc cgccacaatc agtgcaggcc gcagcaactc cgcctctccg gcgaatggac
     4261 attaagacgt ttacaaacat gaaggctccg aagaccgaca cgcagtttac tgccgtagtc
     4321 gcgtacttct atcaaatcga gtctcccgag gaccaacgca aagaatcgat caacattgaa
     4381 acaatgaaag aggctgcgcg acttgtcggc ggcaggcggc aacctccaaa ctggaaattt
     4441 acgctgcaga atgcgcgaaa tgccggctac ctcgaagcag cgggtgacgg aaactacagg
     4501 ctgactccag ttggtgaaaa cctcgtcgcg atcacgcttc ctggaaatgc cgttgctggt
     4561 ggaggcaacg gcgcgggcaa aaagaaaaag tcgtcgaaga aagccggaaa aaaggcaaca
     4621 cgcaaaaagg cggccaaaaa ccgctgatgc gcaagtctcc caattaataa ggaaccttgc
     4681 gccaatgccc actgttcacg aagttcttga tgcggcggtc aatgaagcga cggccgcacg
     4741 tacccgagta aatagggtta ggaccgttca ggtgcgccgc gtcgacgacg tagctatgct
     4801 caaagcgact gcccagacgt ggttccatac gcaccgcccc gccgtagcca tcggtgcgcc
     4861 ccacgttgac ctcacaggaa tcgacgaact ttacatgaag gtgctgggtg caacggccaa
     4921 gtacaccgcc aaatccacct accttgcaac attaaaggag gcgaaggagg aactgatagc
     4981 gatacgagct gcagcgcttg ttgcgcctcg cccatccgca gtcgcaaaca ctgacgacct
     5041 tgccccagac ttttcgccgc tggctggaaa tcaggagatg cgagatattt tgacgcgccg
     5101 ctggcacgag tgcgccaagt gcgtgaaggc ggaggtgcat ttggctgcca ttgtcatgat
     5161 gggcggcctg cttgaagcgc tcttcgtagc gcgagccaac aagatggcag acaaaacacc
     5221 tctcacatca gcaactgacg ctccaaaaga caagaccacc ggaaagacga tcaactacca
     5281 ggaatggatg cttgattccc acatcaaggt cggttttgag ttggggtgga ttactgaatc
     5341 ggccaaaggg gttgctgata agttgaagga gtatcggaat ttcgttcatc cagcgaagga
     5401 attgcggtac ggggtagcgc tagggttcaa cgactcgtca atgttctggc aggtgactaa
     5461 ggcactggca cggcaactgc tattgagtgc gtgaggactc ggtgcgcggt ctatggcctg
     5521 ctgcatcatc caggctaccg caccaagtac cccgacaacc tcaagcgcga gctgccgcgg
     5581 ataccttttg cccccgaatt ccgccccttc gccaaggtcg gcgaaaatct ggccaaactc
     5641 cacctggact atgaacaact gaagccctat gacctcgatt tcatcgaaac gcccggcctg
     5701 ccgctctcct accgcgtcga agacaagatg cggctttcca aagacaagct ccggctggcc
     5761 gtgaatccct cgctcacgct cgccggcatc ccgcccgagg tgttcgacta ccgcctcggc
     5821 aaccgctcgg ccctcggatg ggtcatcgag cagtaccagg tcagcaccga caaacgcagc
     5881 ggcatcacgt cggaccccac cccctggagt aaattggccg cctcgttgac taggtcgtcc
     5941 gcgtcagcgt ggagacggtc aagattgtgc aggggctgcc ggcagagttt gagccaaagt
     6001 aggcgagcga gcttcgcctc gcgtctccac gggccgtggc actctttcag agtgcgaggc
     6061 aatgacgcga ctcgttaccc agggctgcgt ttgcttgcgc acccagggct gcgcttgcta
     6121 acgcaagctt gccctgggct gtcctgtgtc ggcctttcag gccggggagt gcagcatcgt
     6181 cgctgcggcc cgccggcggt ctgccgggcc tgaaaggccc tgacaggaca gcccagggca
     6241 agttcgcgtc agcgaacgcg gccctgggtg cgctgaaacg acgatcctcc gcactctgaa
     6301 agagtgccac gcgaggcaag atcaatccca aacataccgc tcgtcgtagg caatctcata
     6361 ggcgtcaagc aaatcacgaa actcgtcctg atacgaaacg gccgcatgct tctcctgttg
     6421 ccgccgaagg taa
//
LOCUS       DASUGB010000783         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1891656, whole genome shotgun sequence.
ACCESSION   DASUGB010000783 DASUGB010000000
VERSION     DASUGB010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891656"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFV87_15030"
     CDS             <1..931
                     /locus_tag="VFV87_15030"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX5105131.1"
                     /translation="QRTRSARYQRGDTRSWKLPHARRAVAADEEWQGKIVRCLYRPFD
                     WRYVFWHPAMIDWPRDEMTRHLLHADFKSQPRGGEGERGREGETGSSPPLPRSPSLAL
                     IARRQQLPTQPCTFFWIADGLALDGVIRSDNRGSESLFPLYSHDTDDGDQPVSRANFE
                     PRIVTKIAKTVQWSWQPLGSGDLTTNFGPEDLLAYIYALFHSPNYRQRYAEELRSNFP
                     RILPPRSAAQFRTFVNFGRELIDLHLLRTLSPSPSLHLSPSINEFRAGGYLALKKWLQ
                     PPHRSPADPQYGQIAAAIARTLEIMDQIDGGMP"
     gene            complement(942..2741)
                     /locus_tag="VFV87_15035"
     CDS             complement(942..2741)
                     /locus_tag="VFV87_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105132.1"
                     /translation="MSTASPLPKIACPHCQGTMKSPGLPAGSKVNCPKCGQAFVLGGS
                     QAAASPPRVPPVPPQKSAAPPAARPAAAVPPPKPAALPRPPTSAAGPQAPTRPRVLEQ
                     PLGPMDAIPTDALSAAMATQPAHRPPREDKLVDPNLLAPPKPKPKPQPTGITVVCRLC
                     GTRMHAPLEKAGQTIQCPDCHTVNEIKAPAPPKEEKKSGPTLDDVPEFGMSDVVERPK
                     YKPLVVPRGEDMILGNLEGADHPPGWSPPTAAPPASGGRKPSEAASHDLRGLTPPARC
                     EGAPVAEGTLTFHPPPHEVDPEISLEAPVERIEIKPELPKAYLEPEAEDKLYDGKYDD
                     DDMLGGGRVNPKSADAWKRAPFLYGLVEFLLYTTTLPSWLGLGLGAGVVLVIGNSAIF
                     AAQDSSPLMQVSAMLLSMVFAVTMGLFIAPFFGACLAIVQETANGLKEVENWPNMNVT
                     EWFFGALQIPAAAFVAGLPGFATTTFLLTGGMSLLFAPLPIVASWSMLFPIVLYSMLA
                     ENSVLSIWSSRTRESLTVASEAWLLFYMYSIGLGIIGGGAAVLAGVAFWPLTMVGAFG
                     LVTIAFLYFRVLGRLMWYAEQKGLKRAQQSPAA"
     gene            2898..>3199
                     /locus_tag="VFV87_15040"
     CDS             2898..>3199
                     /locus_tag="VFV87_15040"
                     /inference="COORDINATES: protein motif:HMM:NF028342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX5105133.1"
                     /translation="MIIWGSTGREVNVGQGDFHCPQCDSQQRFFHNRIARYFTLYFIP
                     LFQISSHGDFITCTRCSGQFTMEVLNYRPPSSSERLAAMLRGDLDSGMPLHMAQQKL"
BASE COUNT          581 a         1096 c          953 g          569 t
ORIGIN      
        1 ccagcgcacc cgctctgccc gctaccagcg gggggacacg cggagctgga agcttcccca
       61 cgcgcgccgg gcggtggccg ccgacgagga gtggcaggga aaaatcgtcc gttgcctcta
      121 tcgcccgttc gattggcggt acgtcttctg gcacccggcg atgatcgact ggccgcgcga
      181 tgaaatgacc cggcatctgc tgcatgccga ctttaagagt cagccacgag ggggagaggg
      241 ggagaggggg agagagggag aaactggatc gtctccccct ctcccacgct ctccctctct
      301 tgccctcatc gcccgccgcc agcagctccc cacgcagccc tgcacgtttt tctggatcgc
      361 ggacggcctg gcgctcgacg gcgtgatccg cagcgacaac cgcggcagcg aatcgctctt
      421 tccgctctat tcgcacgaca ccgatgatgg ggaccagccg gtgagccgcg ccaatttcga
      481 gcctcgaatc gtcacgaaaa tcgcgaaaac cgtgcaatgg agctggcagc cgctcggcag
      541 cggcgacctg acgaccaact tcggcccgga ggatctgctg gcttacatct acgccctctt
      601 ccactcgcca aactatcgcc agcgctacgc cgaagagctg cggtccaact tcccgcgaat
      661 cctgccgccc cggtcagccg cacagttccg cactttcgtc aatttcgggc gcgaactcat
      721 cgacctgcac ttgcttcgca ccctctcccc ctctccctct ctccacctct ccccctcgat
      781 caacgagttc cgcgccggcg gctatctcgc gctgaaaaag tggctccagc cgccgcaccg
      841 ctcgccggct gatcctcaat atggccagat cgccgcggcg atcgcccgca cgctggagat
      901 catggatcag atcgacgggg gcatgccctg agttggcccg cctatgccgc aggcgactgc
      961 tgcgcccgct tcagtccctt ttgctcggca taccacatca atcggccgag gacgcgaaaa
     1021 tagagaaacg cgatcgtcac caggccaaac gcccccacca tcgtgagcgg ccagaatgct
     1081 accccagcga gcacggcggc gccaccgccg ataatcccca gtccgatgga atacatgtaa
     1141 aacaagagcc acgcctccga cgcgacggtc aggctctcgc gtgtgcggga cgaccagatg
     1201 ctcaagacgg aattttcagc cagcatcgaa tagagcacga tcgggaacag catgctccaa
     1261 ctcgctacga tgggcagcgg tgcaaacagg agcgacatcc cgcccgtcag cagaaaggtt
     1321 gtcgtggcaa aaccaggtag tcccgccacg aacgctgccg cggggatctg caatgcgcca
     1381 aagaaccact cggtcacgtt catgttcggc cagttctcaa cctcttttaa tccattggct
     1441 gtctcttgga cgatcgccag acaagcgcca aagaacggag cgatgaacag gcccatcgtg
     1501 acggcaaaaa ccatcgacaa gagcatcgca gatacctgca tgagcggcga cgaatcttgg
     1561 gcggcgaaga ttgccgagtt gccgatcacc agcaccacgc ccgctcccag gcccagccca
     1621 agccaggacg gcaatgtggt cgtatagagc aggaattcga ctagcccata gagaaaaggc
     1681 gctcgcttcc aggcgtcggc gctcttggga tttacgcggc cgccacccag catgtcatcg
     1741 tcgtcgtact tgccgtcgta aagtttatcc tcggcttcgg gttccaagta cgccttcggc
     1801 aactccggct tgatctcgat ccgctcgacc ggtgcttcca gcgagatctc gggatcgacc
     1861 tcgtgcggcg ggggatggaa cgtgagcgtc ccctcagcga ccggagcacc ttcgcagcga
     1921 gcggggggcg tgagccccct gagatcatgc gacgcggcct cagagggctt acgccctccg
     1981 ctcgccggcg gcgcggccgt cggcggcgac cagccgggcg gatggtcggc cccttccagg
     2041 ttgcccagga tcatgtcctc gccacgcgga acgaccagcg gcttgtactt cggccgctcg
     2101 accacgtcgc tcatgccgaa ttccggcaca tcgtcgagcg tcggtcctga cttcttttct
     2161 tccttcggcg gagcgggagc tttgatctcg ttgaccgtgt gacaatcggg gcactgaatc
     2221 gtctgccccg ccttctcgag cggcgcgtgc atccgcgtcc cgcacagccg gcaaacaacc
     2281 gtgatccctg tgggctgcgg cttcggcttg ggtttgggcg gcgcgagcaa attcggatcg
     2341 accagcttgt cctcccgcgg cgggcgatgg gccggctgcg tcgccatcgc cgccgagagg
     2401 gcgtcggttg ggatggcgtc catcggcccc agcggctgtt cgagcacgcg cggccgcgtt
     2461 ggtgcctgcg gcccagcggc actggtggga ggtcgcggca gtgcggccgg cttgggcgga
     2521 ggcaccgctg cagcgggacg agcggctggc ggcgcggccg acttttgcgg aggtaccggc
     2581 ggcacccgtg gcggactagc agccgcttgc gacccgccca gcacgaacgc ctggccgcac
     2641 ttggggcaat tcaccttcga ccccgccggc agcccgggcg acttcatcgt cccctggcaa
     2701 tgcgggcaag caatcttcgg cagcgggctg gcggtggaca tcgacgagcg gctccgtacc
     2761 agtcattcta acgcctcaac tcaccggact cgacgcattt cgactccgtt gtcgatttct
     2821 tccgccgctc gcagtacact ctcactgatg gcaggcgtgc cgtcccaagc caacggttcg
     2881 atcacgagag actcgccatg atcatctggg gttcgacggg ccgtgaggtg aatgtcggtc
     2941 agggcgattt tcactgcccg caatgcgatt cacagcagcg cttctttcac aaccgcatcg
     3001 cccgctactt tacgctctat ttcattcctc tgtttcaaat ctcgagccac ggcgatttca
     3061 tcacctgcac gcgctgcagc gggcagttca caatggaggt cctcaactac cggccgccct
     3121 cttccagcga gcggctggcg gcgatgctcc gcggcgacct tgattccgga atgccgctgc
     3181 acatggctca gcaaaagct
//
LOCUS       DASUGB010000784         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1474897, whole genome shotgun sequence.
ACCESSION   DASUGB010000784 DASUGB010000000
VERSION     DASUGB010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474897"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /gene="hrpA"
                     /locus_tag="VFV87_15045"
     CDS             complement(<1..1508)
                     /gene="hrpA"
                     /locus_tag="VFV87_15045"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX5105134.1"
                     /translation="MSYYQNLEAQIATAMQADRHRLRNLLRAVRKAEDEGRPPDQRLE
                     KLLGQIEESNARREARQALVPKLDYDEALPIVAHREEIAVAIRDHQVVVVCGETGSGK
                     STQLPKICLEIGRGVAGLIGHTQPRRIAARSIASRLAEELGTSVGQKVGFKIRFTDAT
                     GPQTLIKVMTDGVLLAESQHDRFFDQYDTLIIDEAHERSLNIDFLLGYLKRLLAKRDD
                     LRVIITSATIDAERFATHFAQSRDQWSRSPAGAVGTATTGRGFEAAPIIEVSGRTYPV
                     DVLYRPLMQEDGEEQIDPIRGVADAVEEVCRLGGGDVLVFLPTERHILEASHKLRGRN
                     LPGGKPDILPLYARLSTAEQNRVFQPHSGRRIVLATNVAESSLTVPGIRYVVDCGTAR
                     ISRYSARSKLQRLPIERISQASADQRKGRCGRIGPGVCIRLYDEDDFLSRDKFTSPEI
                     QRTNLASVILQLLALDLGLIDEFPFLDSPRADAIHDGYKTLFELNAIDEQRNL"
     gene            complement(1515..>2067)
                     /locus_tag="VFV87_15050"
     CDS             complement(1515..>2067)
                     /locus_tag="VFV87_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5105135.1"
                     /translation="GRGENTAAILRISLPMGVSFNGHAGAIDWIQSRFKKGKPATLYY
                     DEVRTPTYTDCLNPLLESVLARPDITGLFHAGGPRRLSLYQIAQIVNRIGGYDPRLLY
                     GCPRIDAGPIPPRAGDVTMNSSKLARALGYDPFDPWPLAEEHVPTHRQWHYEGPRGSP
                     GLLKDVLYRNPRYHRTVLAAGGL"
BASE COUNT          343 a          673 c          666 g          385 t
ORIGIN      
        1 agattgcgtt gctcatcgat cgcattcagc tcaaacagcg tcttatagcc gtcgtggatc
       61 gcgtcggctc gcggcgagtc gagaaacggg aattcgtcga tcaagcccag atcgagcgcc
      121 aaaagctgca ggatcaccga tgccaggttc gtccgctgaa tttccggcga cgtgaacttg
      181 tcgcggctga gaaagtcgtc ttcatcgtac agccgaatgc atacgccggg cccaatccgc
      241 ccacagcgac cctttcgctg gtcggccgag gcctgcgaaa tccgctcgat cggcagccgc
      301 tgcagcttgc tgcgggccga atagcggctg atgcgggcgg tgccgcaatc gacgacgtaa
      361 cggatgcccg gcacagtcag cgacgactca gccacgttcg ttgccagcac gatccgccgg
      421 cccgaatgcg gctgaaacac gcggttttgc tcggccgtcg aaaggcgggc atacagcggc
      481 aggatgtcgg gcttgccacc cggcagattg cgaccgcgga gcttgtgcga cgcttccaga
      541 atgtgccgct cagtcggcag aaacaccagc acatcgccgc cccccagtcg gcagacttct
      601 tcgactgcat cggctacgcc gcggatcggg tcgatctgct cttcgccgtc ttcctgcatg
      661 agcggccggt agagcacgtc caccggatac gtgcggccgg agacttcgat gatgggggcc
      721 gcctcaaagc cgcgaccagt ggtcgcggtt cccactgcac cggccggcga ccgcgaccac
      781 tggtcgcggc tttgggcgaa gtgcgtcgcg aaccgctcgg cgtcgattgt ggcgctggtg
      841 atgatcaccc gcaggtcgtc gcgcttcgcc agcagccgct tcaggtagcc gagcagaaag
      901 tcaatgttca gcgagcgctc gtgggcctcg tcgatgatca gcgtgtcgta ctggtcgaag
      961 aaccggtcgt gctgcgactc ggccaggagc acaccgtcgg tcatcacttt gatgagcgtt
     1021 tgcggcccgg ttgcgtccgt aaagcgaatc ttgaagccga ccttctgccc gaccgacgtg
     1081 cctagttctt ctgccaatcg cgaggcaatc gagcgggccg caatccgccg cggctgcgtg
     1141 tggccgatca agccggccac tccgcggccg atttccaggc agattttcgg aagctgcgtc
     1201 gatttgccac tccccgtctc gccgcagacg acgactacct gatggtcgcg aatcgccacg
     1261 gcgatttcct cgcggtgggc cacgatcggc agcgcttcgt catagtccag cttcggaacc
     1321 agcgcctgcc gggcctcccg ccgggcattg gattcctcga tctggccgag tagtttctcc
     1381 agccgctggt caggcggccg gccttcgtcc tccgccttcc gcaccgcgcg gagcaggttc
     1441 cgcagccgat ggcggtcggc ttgcatcgcg gtggcgattt gcgcttcgag attttgataa
     1501 tacgacatca ccgcttacag accgccagcc gcgagcacgg ttcggtggta ccgcggattg
     1561 cggtagagca cgtccttaag cagcccgggc gagccgcgcg ggccctcgta gtgccactgg
     1621 cggtgcgtcg gcacgtgctc ctcggccagc ggccaggggt cgaacgggtc gtaacccagc
     1681 gcgcgggcca gtttgctact gttcatcgtc acgtcgccag cccgtggggg aatcggcccc
     1741 gcgtcaatcc gcgggcagcc gtacaggagg cgcgggtcat agccgccgat acggttgacg
     1801 atctgggcga tctgatacag cgacaatcgc cgcggcccgc cggcgtggaa caggccggtg
     1861 atatccggcc tggcaagcac gctttccagc agcggattga ggcagtcggt gtaggtcggc
     1921 gtgcggactt cgtcgtaata gagcgtcgcc ggcttgccct tcttgaagcg gctttgaatc
     1981 cagtcgatcg ctccggcgtg gccgttgaag ctgactccca tcgggagcga gatgcggagg
     2041 atcgccgcgg tgttctcccc tctcccc
//
LOCUS       DASUGB010000785         6584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_641236, whole genome shotgun sequence.
ACCESSION   DASUGB010000785 DASUGB010000000
VERSION     DASUGB010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641236"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2327)
                     /locus_tag="VFV87_15055"
     CDS             complement(<1..2327)
                     /locus_tag="VFV87_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5105136.1"
                     /translation="MFLLLASFGAIRLPAEEPSQDSLDRDYAGELPRIAPKAPGEALA
                     TFQVAPGFQVEQVAAEPLVTDPVAICFDENLRMFVCEMRGYSENRDDNVSRITCLEDT
                     DGDGKFDQSTVFAEKLAWPTALFWWKGGLIVADAPDVYYFTDTDGDGKADERQVLFTG
                     LGTSNVQGLVNSFQWSLENRIYVSLSSTGAELKHGNADQAQATTYRGRDLAIEPHTWA
                     TQPISGGAQHGMSLNDWGDRFLCSNSDHLQQVMYEDRYLGRNPYLAAPSPRRSIAADG
                     PQADVFRTSPVEPWRIVRTRLRAQKIVPGIVEGGGRPAGYFTGATGATIYRGNAWPKE
                     WRGLAVVGDVGSNLVHRKRLEAEGVGYIGRRIDEKSEFVSSSDIWFRPVQFANAPDGT
                     LYILDMYREVIEHPASLPPIIKKHLDLTSGRNRGRIYRIVPDGFRQPALTKLGDLKTA
                     ELVALLEHPNGWHCDTAARLLYERQDRSAIGPLATMASSSTSPLARMHALHALAGLNA
                     LSEDMLLPRLSDEHPRVREHAVALAEKLLASAAIRARLVVLAGDPELRVRYQTAFSLG
                     ELPTSIQRNRALAAIAGRDADDGYVRIAVLSSLGEGAGEVLSLLIADPVYRVSKAAPE
                     LLGSLAMQIGKQQRAEDVAETLKSLAALAKDNSPLLPVIVQKLAAKDGSPLAEQIAAA
                     TGGKAEAVMQALLAQASTAAADEEAPLAKRVAAAEQLRLAKFADQRELLSALLGPSVP
                     PDLQSAVLRTLASYDAEAVAGLTLSRFAGFSPRLKG"
     gene            2525..3841
                     /locus_tag="VFV87_15060"
     CDS             2525..3841
                     /locus_tag="VFV87_15060"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5105137.1"
                     /translation="MTDKTAPQPTFEGLGLSEVMRAALDKAAYQYPTPVQAGLIPHAL
                     KGVDVMGQARTGTGKTASFGIPILETIELNHDIHHPQALVMVPTRELAVQVKDEIVKL
                     SAGRRVHCVAVYGGKPIREQIEKIRKGAQIIVGTPGRVLDHLARGTVDFSKLKIVVLD
                     EADRMLDIGFRPDIERILKRCPQSRQTLLLSATVPPEIQKLAERYMRDPIALNFSSKD
                     LAVETIEQFYFTVDPQRKFDLLLRLLAREKPKQAIIFCRTKRGTEKIFQRLSKKVKDQ
                     RLVAAIHGDMAQSARDRVMSAFRAGKIRFLVATDVIGRGIDVSSISHIVNYDIPSFCD
                     DYVHRVGRTGRMGREGVAYTFVAPDEGYELTRIEERINRLLKRDEIKDFDPRARENRP
                     LVAAVAGSGAESDDDGSPAPESASPAPPPPPSSAMRRPPKKYRRAL"
     gene            complement(4242..4571)
                     /locus_tag="VFV87_15065"
     CDS             complement(4242..4571)
                     /locus_tag="VFV87_15065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5105138.1"
                     /translation="MGMVIRRFDVLLVALDPTVGSEMRKTRPCLVVSPDQFNRNLQTV
                     LVAPLTTRSRSYPFRAPCRFQGKSGQVALDQMRAVDRSRLVKRLGRFGGKAQSRVLQI
                     LQQMFAP"
     gene            complement(4559..4813)
                     /locus_tag="VFV87_15070"
     CDS             complement(4559..4813)
                     /locus_tag="VFV87_15070"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5105139.1"
                     /translation="MKAKLIKVGNSRGIRLPKAIIEQAGLGDEVELDVRGDQVVIRAK
                     TDPREGWEEACRRMHECGDDELLDGPLPSLTKFDAEEWEW"
     gene            4991..5443
                     /locus_tag="VFV87_15075"
     CDS             4991..5443
                     /locus_tag="VFV87_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105140.1"
                     /translation="MFSRMSIVLGIVALLAAVTVYAADEVKLEGVKCIVNAKAAAKAD
                     NAVEYKDGKVFFCCMNCPKAFAADKVKFATKANHQLVATGQAKQEKCPFSGEEIDANT
                     KITVEGAAICFCCDMCKAKAQESKEAVELLFNDKAFEKAGFKVAKAEK"
     gene            5674..>6584
                     /gene="lpxB"
                     /locus_tag="VFV87_15080"
     CDS             5674..>6584
                     /gene="lpxB"
                     /locus_tag="VFV87_15080"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX5105141.1"
                     /translation="MKLFFSAGEPSGDLHGANLIAALKGRHAALSAVGFGGSRMGSAG
                     CRLLHDMSDLAVMGFGPALAKLPTFYRLKRQAERSFDEHRPDGVVLIDYPGFNWHLAR
                     AAKRRGIPVIYYGLPQLWAWASWRVKKVRKYVDHALCKLPFEEAWFRGNDCNATYVGH
                     PYFDELASQRLDEQLVASLRDRPGRVLTILPGSRPQEVQNNLPMLLQAAALVRRETPA
                     VRVVVASYNKRQAAMARQLVASGPVPAEVFTSRTPELISAADCCLATSGSVSLELLYH
                     AKPAVIVDKVSWLAAQVVRRLVSTRYIT"
BASE COUNT         1168 a         2177 c         2037 g         1202 t
ORIGIN      
        1 cccttcagac gcgggctgaa cccggcgaaa cgcgagaggg tcagccctgc gacagcctcg
       61 gcgtcgtacg aggccagcgt gcgcagcacg gcggattgca gatcgggcgg aaccgacggg
      121 ccgagcaatg ccgacagcag ctcgcgctga tcggcgaact ttgccaggcg aagctgctcg
      181 gcggcggcga cgcgcttcgc gagcggcgct tcctcatccg cggctgcagt agaagcttgt
      241 gcgagcaggg cctgcatgac cgcttcggcc ttgccaccgg tggctgcggc aatctgctcg
      301 gccagcggcg acccgtcctt ggcggccagc ttctgcacga tgaccggcag gaggggcgaa
      361 ttgtccttgg cgagcgcggc cagcgacttg agcgtttcgg ccacgtcttc ggctcgctgc
      421 tgcttgccga tctgcatcgc caggctgccg agcagctctg gcgcagcctt ggaaacgcgg
      481 tacaccggat cggcaatgag cagcgagagc acttcgcccg ccccttcgcc cagcgaactt
      541 agcacggcga tccgcacgta accgtcgtcg gcgtcgcgcc cggcaatcgc ggccagcgcg
      601 cggttccgct ggatgctcgt cggcagctcg cccagcgaaa aagcagtctg atagcggacg
      661 cgcagctccg gatcgcccgc cagcaccacc agcctggcgc ggattgccgc cgaagccagc
      721 agcttctccg ccagggcgac cgcatgctcg cgcacccgcg ggtgttcgtc gctcagccgc
      781 ggcaggagca tgtcctcgct gagggcgttc agccctgcga gcgcatgaag tgcgtgcatc
      841 ctcgccagcg gcgaagtgct gctcgacgcc attgtcgcca gcggcccgat cgccgatcga
      901 tcctgccgct cgtacaacag tcgagccgcc gtatcgcagt gccagccatt gggatgttcg
      961 agcaaggcga cgagctccgc cgttttcaga tcgccgagct tcgtcaacgc aggctgccgg
     1021 aagccatccg gcacgatgcg gtagatcctc ccgcggttgc ggccgctggt caggtcgagg
     1081 tgcttcttga tgatcggtgg caggctggcc gggtgctcga tcacctcgcg gtacatgtcg
     1141 aggatgtaga gcgttccgtc gggagcattg gcgaattgca ccggccggaa ccagatgtca
     1201 ctcgacgaga cgaattcgct cttctcgtcg attcgccggc cgatgtagcc gacgccttcc
     1261 gcctccagcc gcttgcggtg gacgaggttg ctgccgacgt cgcccaccac cgctaatccg
     1321 cgccactcct ttggccaggc gttgccgcga tagatcgtcg cgcccgtggc gccggtaaag
     1381 tagcccgccg gccggccgcc tccttcgacg attcccggca cgatcttctg cgcccgcagc
     1441 cgcgtgcgga cgatccgcca cggctcgacg gggctggtgc ggaagacatc ggcctgcggg
     1501 ccgtcggctg cgatgcttcg ccgcggcgac ggggcggcca ggtacggatt gcggcccaga
     1561 taccggtcct cgtacatcac ctgctgcagg tggtcgctgt tggagcagag gaaccggtcc
     1621 ccccagtcgt tcaacgacat cccgtgctgt gccccgccgc tgatcggctg agtcgcccag
     1681 gtgtgcggct cgatggcgag atcgcgaccg cgatacgtgg tggcctgcgc ctggtccgcg
     1741 ttgccgtgct tcagctccgc tcccgtgctg ctgagcgaga cgtagatgcg gttttccaag
     1801 ctccactgga acgaattgac cagtccctga acgttgctcg ttcccaggcc cgtgaacagc
     1861 acctgacgct cgtccgcctt gccgtcgccg tcggtgtcgg tgaagtaata gacatccggt
     1921 gcatcggcga cgatcagccc ccccttccac cagaacaaag ccgtcggcca ggcgagcttc
     1981 tcggcaaaga ccgtgctctg atcgaattta ccgtcgccgt ctgtatcttc caggcacgta
     2041 atccggctga cgttgtcgtc gcgattctcc gaatatcctc gcatttcgca gacgaacatc
     2101 cgcaggttct cgtcgaagca gatcgcgacc ggatcggtca ccagcggctc cgcggcgact
     2161 tgctcgacct ggaaccccgg cgcgacctga aacgtcgcca gtgcctcgcc cggtgccttg
     2221 ggcgcaattc gcggaagctc gccggcatag tcccgatcga gcgaatcctg ggagggctcc
     2281 tcggcgggca aacgaatcgc cccaaacgag gctaacagta gaaacacaca gccaaccgtc
     2341 ctgctcattt ccctcatcgc ggcacgcacc cgattaaatg gccaaaactc aacgtgaacg
     2401 tatcattgca aggctgcaga ttgcattcaa tcacgttgac ggtttgcggc gggcggggta
     2461 ggttttccta aatcaattcc agcaaagcct ttacacgcga tcgtctcgcc aggaacctac
     2521 ggatttgacg gacaagaccg ccccccagcc caccttcgag ggcctcggac tctccgaggt
     2581 catgcgggcc gccctcgaca aggccgccta tcagtatccc acgccggtcc aagccgggct
     2641 cattccccat gcgctcaagg gcgtggacgt gatgggccag gcccgcaccg gcacgggcaa
     2701 aaccgcctcg ttcggcatcc cgatcctgga aacgatcgag ctgaatcacg acatccacca
     2761 cccgcaggcc ctggtgatgg tgccgacgcg cgagctggcc gtgcaggtca aggacgagat
     2821 cgtcaagctc tccgccggcc gccgcgtcca ctgcgtcgct gtttatggcg gcaagccgat
     2881 ccgcgagcag atcgagaaaa tccgcaaggg ggcccagatc atcgtcggca cgcccggccg
     2941 cgtgctcgac cacctggccc gcggcacggt cgatttcagc aagctcaaaa tcgtcgtcct
     3001 cgacgaagcc gaccggatgc tcgatatcgg ctttcggccg gacatcgagc ggattctgaa
     3061 gcggtgcccg caatcgcgcc agacgctgct gctctcagcc acggtgccgc cggagatcca
     3121 gaagctggcc gagcgctata tgcgcgaccc catcgcgctc aacttctcgt cgaaggacct
     3181 ggccgtcgag acgatcgaac agttctattt cacggtcgat ccgcaacgga agttcgacct
     3241 gctgctaagg ctgctcgcgc gtgaaaagcc caagcaggcg atcatcttct gccgcaccaa
     3301 gcgcggcacc gagaagatct ttcagcggct ctcgaagaag gtgaaggacc agcggctggt
     3361 ggcagcgatt cacggcgaca tggcccagag cgcccgcgac cgcgtgatga gcgccttccg
     3421 ggcggggaag attcgcttcc tcgtggccac cgatgtgatc ggccgtggaa tcgacgtctc
     3481 gagcatctcg cacatcgtca actacgacat tcccagcttc tgcgacgact atgtccaccg
     3541 cgtcggccgc acggggcgga tggggcgcga gggcgtggcg tacacgttcg tcgctcccga
     3601 cgaaggctac gagctgacgc ggatcgaaga gcggatcaac cgcctcctca agcgcgacga
     3661 gatcaaggac ttcgaccccc gcgcccgcga aaaccggccc ctcgtcgccg cggtcgcggg
     3721 gagcggtgcg gaatccgatg acgacggcag ccccgcgccg gagtccgcca gccccgctcc
     3781 gcctccgccg ccatcgagcg ccatgcgccg cccgccaaaa aagtaccgcc gggcgctgta
     3841 gctcatcaag cttacgatgc agccgaacgc accgccgatg ctcggtcaga ttgtcggcgg
     3901 gattctcgcg gtgatcttct gcggttcgtt gacggctttg gctgtttggg gagcctggcg
     3961 aaatcgggac ttgcccgcct tcgagtcctg gcggagttcc tcctcgtccc agtttcgcaa
     4021 tctcgtttgg gtttgccgaa tcaacggtcc attggttctt ctgatgtcat gctttgcgct
     4081 atttgccagt cccggcctgc tgtgggacga gtcagtccct tggctggcgg cgcgaactat
     4141 cattgcactc ttgggattac tgttgtcggc cagttgggtt tttctggctt ggcgctggac
     4201 gaggtggggt tggtcggtcc gcctctaatc cagatggaat gctacggcgc aaacatctgc
     4261 tgcaggattt gcagcacccg cgactgcgcc ttgccgccga aacgccccag gcgcttcacc
     4321 aaacgcgagc ggtcgacggc ccgcatttgg tcgagggcga cctgccccga tttgccttga
     4381 aatcggcagg gtgctcgaaa cggatacgac cggctgcggg tggtcagcgg ggccacgagc
     4441 accgtctgca gattccggtt gaattggtcc ggcgacacaa cgagacacgg ccgcgttttc
     4501 cgcatttcgc tccccacggt cggatcgagc gccaccaaca gcacgtcgaa ccgccggatc
     4561 accattccca ttcctctgca tcgaatttgg tcagggacgg cagcggccca tcgagcagtt
     4621 cgtcgtcgcc gcactcgtgc atccgccggc aagcttcttc ccagccttca cggggatccg
     4681 tcttcgctcg aataacgacc tggtcacccc gcacgtccaa ctcaacctca tcccccagcc
     4741 ccgcttgttc aatgatggcc ttgggcagtc gaatccctcg agaattgccg acttttatca
     4801 gtttcgcctt cattgcccct gctccgttgg tatgtgatta ctttgtatat acgcccgaaa
     4861 ccggccacgg tcaacgcgag cacagcgaag ccatcctgtt gacgcggcgc ccgcatcgca
     4921 gtcagaatgg ccacctgcaa cggagccagg ttcgcacgac gcggctaatt gccaatcagg
     4981 agttcttgcc atgttctcgc gcatgtcgat cgttttggga attgttgctc tgctggccgc
     5041 ggtgaccgtc tacgccgccg acgaggtcaa gctcgagggc gtcaagtgca tcgttaacgc
     5101 caaggcggcc gcgaaggcgg acaatgcggt cgagtacaag gatggcaaag ttttcttttg
     5161 ctgcatgaat tgcccgaagg cgttcgcggc cgacaaagtc aagttcgcga cgaaggcgaa
     5221 ccaccagctt gtcgccaccg gccaggcaaa gcaggaaaag tgccccttca gcggcgagga
     5281 aatcgatgcc aataccaaga tcaccgtcga gggggcggcc atctgcttct gctgcgatat
     5341 gtgcaaagcc aaggcgcagg agtcgaagga ggcagtcgag ctgctcttta acgacaaagc
     5401 ctttgagaag gcgggcttta aggtggccaa ggccgaaaag taaggaatag gccgctagcg
     5461 tcgcctgccc gcgagcaaca cgtaggacgg gtttccaacc cgtcccggac aggttgaaat
     5521 cctgtcctat gacacgctac tggcatgcca gcacccgccg tttccgcgtc taaactgccc
     5581 taggtccagc cgcaccgttt tgcaatactc cgcgcggccc aggcctgccc aacctggact
     5641 cctggagcgg gactcttgca ggacgcggcg gcgatgaagc tatttttctc ggctggcgaa
     5701 cccagcggcg atctccacgg cgcgaatctc atcgcggcgc tcaaaggccg tcacgctgcg
     5761 ctgtcggctg ttggatttgg cggctcgcga atgggctccg ctggctgtcg tctgctgcac
     5821 gatatgagcg acctggccgt gatgggattc ggcccggctt tggcgaagct gccgacgttc
     5881 taccggctca agcggcaagc cgagcgttcc ttcgacgagc accggccaga tggtgtcgtc
     5941 ctgatcgact atccgggctt caactggcac ctcgcgcggg cagccaagcg ccggggcatt
     6001 cccgtcatct attacggcct gccacagctc tgggcctggg cctcgtggcg ggtgaagaag
     6061 gtccggaagt acgtcgatca tgcgctatgc aaacttcctt tcgaggaagc atggttccgc
     6121 ggcaacgact gcaacgccac gtatgtcggc catccgtatt tcgacgagct ggcgagccag
     6181 aggctggacg agcagctcgt ggccagcctg cgcgaccggc cgggacgcgt gctgacaatc
     6241 ctgcctggct cacgacccca ggaagtgcag aacaatctgc cgatgctcct gcaggcggcg
     6301 gctctggtgc ggcgtgaaac gccggccgtg cgagtcgtgg ttgccagcta caacaagcgg
     6361 caggccgcga tggcccggca gctcgtcgcc agcggaccgg tgccggcgga agttttcacc
     6421 agccgcaccc ccgagttgat ctccgcggcc gattgctgcc tggcgacgag cggctcggtg
     6481 tcgctggagc tactgtatca tgccaagccg gcggtgatcg tcgataaggt ttcgtggctc
     6541 gcagcccagg tggtccgccg gctggtgagc acgcgctaca tcac
//
LOCUS       DASUGB010000786         5812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1753869, whole genome shotgun sequence.
ACCESSION   DASUGB010000786 DASUGB010000000
VERSION     DASUGB010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753869"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..>5812)
                     /locus_tag="VFV87_15085"
     CDS             complement(51..>5812)
                     /locus_tag="VFV87_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5105142.1"
                     /translation="GQSLSDGQEVIDFGTATAGTPQSITLTLQNLGSAPLVIPSGGLA
                     LPSGYSLAESLPESIEPGGSVAVTINLLVLAPGDYSGTLALTTNDEDESPFEIELTGI
                     VQAANPQIEILDEDDQPLASSGTLAFGATSPGVAVTRTLTIVNSGTTPLVLDLQSLSL
                     PAGYSLMLTPAATVEPGDWTPLAIQLDADELGNFSGELSLASNDAAASPFVLQLTGSV
                     NPPPPEIEVLAGSSLSALEIGQTLDLGQTLVGQPIQVRGYIRNAGTGLLTIDVGSIAI
                     PAGFALAAAPAATVEPGQWTEFTLELAATASGAFSGIVSIPNSDPDESPFQFTVQGQV
                     NPPAPVAMVRILGRAVPAMGSAVRSLGVAVVGTPLRGTFVIANEGTASLEVTNVALPS
                     GFTLVSSPAAPIPAGAQGEVVVELTAASAGTFEGDLTFATNDTAAASFAIPIRGTVVA
                     SATQIEVREGATLLASGATIDLGSIMAGTATSVTLEIRNVGSSTLVLDPAAIALPAGL
                     TLTGVPAETVAPSGATNLTVSLDTSLPASFSGTLSFSSNGQSTPFRVTLLADVLPSLT
                     GLGIGSVELENDDGASNTDRVTTDPRLRVQLVNAQAEELPQLRVEVDLDNDDLPEASA
                     AVAADSVSIVQLDERSLAAGPVAARVRLTNQTTGAATDWQAFSFTYELPDMLPTVALQ
                     LAEDTGTPGDLITSNPIVAGTVTVAAGTAANLEVAIDLNGDDKADFSTRTDALGAFSV
                     DPRQILPQLAAGQITVRAQARQFDPRRQTLAANDWVSLTFEYAIVRQLPQVQNLQADQ
                     SASDLAFGEVRITGGVVDPDAQTRFLSVEYDIDGDGTVDGEVYASPIDGSPFDFVVEA
                     KQLPAGERTIQVRGVKLVRQTAERIVGNWQSVTFQNTISQPALPLLSALAFQASQDAD
                     GSKNHAIAGHVSRSGAPAGGATVEYDLDGDGQRDGLTFSRHDADGSFQLDLSRHGLLP
                     GAHTVQLRARAWDADRRQMVASDWQEFTFTIDPIAQPVLPDLQSLTLAVDTGTPGDGI
                     TSLPKIAGTAQWADETFGDFVVEYELLGENSGSGRVIALAALDGEFVLDLSHIQFADP
                     QIELRLRARGWNASRQELVSEWEDFAFTLSSSANVLPSITAVGLLEDTGTAGDNVSTN
                     ATLTGSVSHPDGSASVVPVQYDLNSDGIADGFVNTMPDGTFSLDLNARFTTQGPRTVA
                     LRAGELNAAGQYVYGGWTSFSFTVERAGPPPTAVVTSLRLLNDTGEDDDDGITSDPRI
                     TGTVVLSDGSLAAGLPVEIDYTGDGYSDEIAVTDPTGGGFVADLRDRIHEAGPVTVRF
                     RAGVPAETVSGFQYGEWTSLTFEFELDPIAPSTTPEASLPPLDVTVIPPSPPPYAQYY
                     ADPILFAYGELYGILTLADIAGGQFDLFGSRFGPHPQGNVSTVVTVDESDTQTYPGSE
                     PDGGTLTVTTTLNSTLTTNATLGGDGTWTVSEILVSTYLIVKTYSSTSGLSYTLTQSG
                     THNYTFTASGDADDALYSLNETRLDEWSSDREDPESTSGGLTVSVVSSGESSQGFTFI
                     ASGWRLFPSGGSMTEHEDWDYTSTADGEYSITTTTGDDAVVEESSHSATGHDLIVTDL
                     VSGVRTTVRNFLHEGQSEASATDGADWSSGSTSSSWDRTVTTATDSRWQGTLTTVDDG
                     LTVSRTGSYDQDQTIIYEWDVDSAWSGSEVIAAPLGSITRLLSNTHHYDKQEKYEHQQ
                     QWQVDDELVTHTGGSTITTITVNTLDHWSADEKQNLTTASAGTTVTHNIQRLTTTDNS
                     YSWLQRNESDYTVSSSGKTESASTSALTTRHIQEQKETETENLSASGKITATGVSNYS
                     AEFSTDITTDTRSGDDNGDTWLSYSEAGERHGLWMHSLTSNISGTSGGGDAPLVSFKF
                     SAQSQSNGSGKQTYSSNAY"
BASE COUNT          950 a         1788 c         2011 g         1063 t
ORIGIN      
        1 ccttcggaat cggttcccag gccgctgtag cccttgccgg gggtgctctc ctagtaggcg
       61 ttggacgagt acgtctgctt gccggagccg ttggactgcg attgggcgct gaatttaaat
      121 gagaccagcg gggcgtcgcc accgccgcta gtgccggaga tgttgctggt gagcgagtgc
      181 atccacaggc cgtggcgctc gccggcttcg gagtaggaga gccaagtgtc gccgttgtcg
      241 tcgcccgagc gcgtatcggt cgtgatgtca gtggagaact ccgccgaata gttggagacg
      301 ccggtcgcgg tgatctttcc agaagcgctt agattctcgg tttcggtttc cttctgctcc
      361 tgaatgtgcc gcgtggtaag ggcggaggtg gaagcggatt ccgtcttgcc gctggacgag
      421 acggtgtaat ccgattcgtt ccgctggagc caggaatacg aattatcggt cgtggtgagc
      481 cgctggatgt tgtgggtgac ggtggttccg gcactggcgg tggtgaggtt ttgcttttcg
      541 tcggcggacc agtggtcgag cgtgttgacg gtaatcgtcg tgatcgtcga tccgccggta
      601 tgcgtgacca gctcgtcgtc aacctgccac tgctgctggt gctcgtactt ctcttgcttg
      661 tcgtagtggt gcgtgttgct gagcaggcgg gtgatggagc ccagcggcgc cgcgatgacc
      721 tcgctgccgc tccaggcact atcgacgtcc cactcgtaaa taatcgtctg gtcctggtcg
      781 tagctgcccg ttcgggagac ggtcaggccg tcatccacgg tggtgagcgt gccctgccag
      841 cgcgaatcgg ttgccgtagt aacggtgcgg tcccagctcg acgacgttga gccgctgctc
      901 caatcggcgc cgtcggtggc gctggcctcc gactggccct cgtgcagaaa gttgcggact
      961 gtcgtgcgaa ctccggagac gaggtcggtg acgatcagat cgtggcccgt ggctgaatgg
     1021 ctcgactcct cgaccaccgc gtcgtcgccg gtcgtcgtcg tgatggagta ctcgccatcc
     1081 gccgtggacg tgtaatccca atcctcatgc tcggtcatgc tgccgccgct ggggaatagc
     1141 ctccagccgc tggcgatgaa ggtaaagccc tgcgagcttt cgccgctgga gacgacggag
     1201 acggtcagcc cgccactggt gctttccggg tcctcgcggt cgctgctcca ttcgtccagg
     1261 cgcgtctcgt tcagcgagta gagggcgtcg tctgcgtcgc cgctggcggt gaaggtgtaa
     1321 ttgtgcgtgc cggactgcgt gagcgtgtag ctcaggccgc tggtgctgga ataggttttg
     1381 acgatgaggt aggtcgaaac caggatctcg ctgaccgtcc aggtgccatc gccgccgagc
     1441 gtggcgttgg tggtcagcgt cgagttgagc gtggtcgtaa cggtcagcgt gccgccgtcg
     1501 ggctcagagc cgggatacgt ttgcgtgtcc gattcatcga ccgtgacgac cgtgctgacg
     1561 ttgccctgcg gatgcggccc gaatcgcgag ccgaacagat cgaactggcc gccggcgatg
     1621 tcggcgagcg tcaggatgcc gtacagctcg ccgtaggcga acagaatcgg atccgcgtag
     1681 tactgggcgt atggcggcgg agaaggagga atgaccgtga cgtccagcgg cggcagagaa
     1741 gcttcgggcg tcgtgcttgg agcgatcgga tcgagctcaa actcgaacgt caaactcgtc
     1801 cattcgccgt attggaaacc ggaaacggtt tctgccggca cgccggcgcg gaatcgcacc
     1861 gtgaccgggc ccgcttcgtg aatccggtcg cgcagatcgg caacaaagcc gccgcctgtg
     1921 gggtcggtga cggcgatttc gtccgaatag ccgtcgccgg tgtaatcgat ttcgacggga
     1981 aggcccgcgg ccaggctgcc atcggagagg acgaccgtgc ccgtgattcg cgggtcgctg
     2041 gtaattccgt cgtcatcgtc ttcgcccgtg tcgttgagga gtcgcaggct cgtcaccacc
     2101 gcagtaggcg gcgggccggc tcgctcgacc gtgaagctga agctcgtcca gcctccataa
     2161 acgtactgcc ctgcggcgtt caactcgccg gcgcggaggg cgaccgtgcg cggaccttga
     2221 gtcgtgaacc gggcattcaa atcaagggag aacgtgccgt ccggcatggt gtttacgaag
     2281 ccgtcggcga tgccgtcgct gttcaggtcg tactgtactg ggacgacgga agccgaacca
     2341 tccggatggc tgacgctgcc ggtgagcgtg gcgttggtgc tgacgttgtc gccggcggtt
     2401 cctgtgtctt ccagaagtcc gacggcggtg atgctcggca ggacgttggc actggacgac
     2461 agcgtaaagg caaagtcctc ccactcgctg accaattcct ggcggctggc attccagccg
     2521 cgagcccgca ggcgcagctc gatctgggga tcggcgaatt ggatgtgtga caaatcgagg
     2581 acaaactcgc cgtccaaggc cgccagcgcg atgacgcggc cggagccgct gttttcgccg
     2641 agcaactcgt attcgacgac gaagtcgccg aacgtctcgt cggcccattg cgcggttcca
     2701 gcgatcttgg gcagagacgt gatcccgtcg cccggcgtgc ccgtatcgac ggccagcgtc
     2761 agcgactgga ggtctggcaa gaccggctgc gcaatcgggt cgatcgtgaa cgtgaactct
     2821 tgccagtccg acgcgaccat ctgccggcgg tcggcatccc atgctcgagc gcggagctgc
     2881 accgtgtgcg cgcctggtaa cagaccgtgg cgactcaagt cgagctgaaa gctgccgtcg
     2941 gcgtcgtgcc gcgagaaagt caatccgtcg cgctgtccgt cgccatcgag gtcgtattcg
     3001 accgtcgctc cgccggcggg tgcgccgctg cgggaaacgt gcccggcaat cgcgtgattc
     3061 ttcgagccgt ctgcatcttg cgaggcctga aacgccaagg cgctgagcaa cggcagggcg
     3121 ggctggctga tcgtgttttg aaacgtcacg gactgccaat tgccgacgat gcgctcagcc
     3181 gtctgccgca cgagcttgac gccgcggacc tgaatcgtcc gctcgccggc gggcaactgc
     3241 ttggcttcga cgacgaaatc gaaggggctg ccgtcgatgg gggaggcata aacctcgccg
     3301 tccacggtcc cgtcgccgtc gatgtcgtac tcgacggaga ggaaccgcgt ttgcgcgtcc
     3361 ggatcgacca cgccgccggt gattcgcacc tcgccgaagg cgaggtccga cgccgactgg
     3421 tcggcttgca agttctggac ctgcggaagc tggcgcacga tggcgtactc gaacgtcaag
     3481 ctgacccaat cgtttgccgc gagcgtttgc cggcgcggat cgaactgccg cgcctgggcc
     3541 cggacggtga tctgccccgc agcgagctgc ggcagaatct gccgcggatc gacgctgaat
     3601 gcgcccagcg cgtcagtccg cgtggaaaag tccgccttgt cgtcgccatt caggtcgatg
     3661 gcgacctcca ggttggcggc tgtgccggcg gcgactgtga cggtcccagc cacgatcgga
     3721 ttggaggtga tgagatcgcc gggtgtcccc gtgtcctcgg cgagctgcag cgccacggtg
     3781 ggcagcatat ccggcagctc gtacgtgaac gagaacgctt gccagtcggt cgcggcgccc
     3841 gtcgtctggt tcgtcagccg cacgcgggcc gcaacgggcc cggccgccag actccgctcg
     3901 tcgagctgca cgatgctcac ggagtcggcg gcgacggctg ccgaagcttc cggcaggtcg
     3961 tcgttgtcga gatcgacttc gacgcgcaac tggggcagct cctcggcctg ggcgttgacg
     4021 agttggacgc ggaggcgcgg gtcggtcgtg acgcggtcgg tattcgatgc gccgtcgtcg
     4081 ttctccagtt ccacgctccc gattccaagt cccgtgaggc tgggcaggac atccgcgagt
     4141 agcgtcacgc gaaatggcgt gctctggccg ttgctggaaa acgagagcgt gccgctgaag
     4201 ctggcgggca aggacgtatc gagcgagacc gtcaggttgg tcgcgccgct cggcgcgacc
     4261 gtttcagccg gaacgccggt gagcgtcaat ccggccggca gggcaatggc cgcaggatca
     4321 aggaccagcg tgctggaccc gacgttgcga atctcgagcg taaccgacgt ggcggtgccc
     4381 gccatgatgc tgcccagatc gatcgtagcg ccgctggcca gcagtgtcgc accttcgcgg
     4441 acctcgatct gggtcgccga ggcgacgacc gttccgcgaa tcgggatggc aaacgaagcg
     4501 gcggccgtat cgttggtcgc aaatgtcaaa tcgccttcga acgtgccggc ggaagcggcg
     4561 gtcaattcca caaccacctc gccctgggcc ccggccggaa taggcgcggc aggtgaactg
     4621 acgagcgtga aaccggacgg cagggcgacg tttgtgacct cgagcgacgc cgtgccttca
     4681 ttagcaatga cgaacgtccc gcgcagcggc gtgccgacga cggccacacc gagcgagcga
     4741 acggccgatc ccattgcggg aactgcgcgg cccaggatcc gcaccatagc cactggcgcg
     4801 ggcggattga cctggccctg gaccgtgaat tggaagggac tctcgtccgg atcgctgttg
     4861 ggaatcgata cgatgccgct gaatgcgccg ctggccgtgg ccgccagctc caacgtgaac
     4921 tcggtccatt ggccgggctc gaccgtggcg gcgggcgccg cggcgagcgc gaagccggcg
     4981 ggaatggcga tcgacccgac gtcgatcgtc aacaggcccg tgccggcgtt gcgaatatag
     5041 ccccgcacct gaatcggctg gccgacgagc gtctggccga gatcgagtgt ctgcccgatc
     5101 tcgagcgcgc tcaggctgct gccagccagc acctcgattt ccggcggggg cggattcacg
     5161 ctgccggtga gctgcaagac aaacgggctg gctgcggcat cgttcgacgc caggctcagc
     5221 tcgccgctaa aattgccgag ctcatcggca tcgagctgga tcgccagcgg cgtccagtcg
     5281 cccggttcga cggtggccgc gggcgtcagc atcaggctgt agcccgcagg cagcgagagc
     5341 gactgcagat cgaggaccag gggcgtcgtc ccgctgttga caatcgtgag cgtgcgagtc
     5401 acggcaacgc cgggagacgt cgccccgaaa gcgagcgtgc cggacgaggc cagcggctgg
     5461 tcgtcttcgt ccaggatctc aatctgtgga ttggccgcct ggacgatccc cgtcagctcg
     5521 atctcgaacg gactctcgtc ctcgtcattc gtcgtgagcg cgagcgtgcc gctgtaatcg
     5581 ccgggcgcca ggacgaggag gttgatcgtc acggccacag agccgccggg ctcgatcgac
     5641 tcgggcagcg attcggccag gctataaccg ctcggcagcg ccaggccgcc ggacggaatc
     5701 accagcggcg cggacccgag attctgcaac gtcagcgtga tcgattgcgg cgtgcccgcc
     5761 gtggccgtgc cgaagtcgat gacttcctgg ccatcgctaa gcgactgccc gt
//
LOCUS       DASUGB010000787         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_920370, whole genome shotgun sequence.
ACCESSION   DASUGB010000787 DASUGB010000000
VERSION     DASUGB010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920370"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="VFV87_15090"
     CDS             complement(<1..1901)
                     /locus_tag="VFV87_15090"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX5105143.1"
                     /translation="MPTTASTKPRSIRSLARALAAHAVQLHDELQVRLLSGDQQLQSA
                     RDSLAADLGREVSVDEAANVIAQAATCGGVIVASKPQEWNSATRWFHVQASDWERCAL
                     QEIRTEYSVLRTQYSVLDIPASSSAHFYETFLHAYQRCTRKQRGVFFTPAPIADFIVR
                     QVDERLRGEFGLAQGLAAAAGPPYREADASRSPCVLDPACGTGVFLLAVIERLHCQLG
                     DDWNAVVPKILPRLIGIEILPAAAFLAKLNIALKLAATGYSFRQPGSINIRIGDALEP
                     NLQFEICNLKSAIPVVVGNPPFSSLSTNTNPWIARLVRGDDVVCGYVRANGRSLGERK
                     TWLHDDYVKFIRLAQWHVEQAGQGIVGFVTNHGYLDNATFRLMRSELLRAFPHIQIVD
                     LHGSRKKGEQSPDGSRDENVFGLDQGTAISILTRSVSEGVTPTRSVSEGVIPRIEYAE
                     LWGSRDEKLAALGTPSLTLRVTIRPAAPDWRFVPTRKTVHPEYDAGWPLTELMPVNTP
                     APVTARDHFVVAFHEEELQRRIAEFRDLAIPDDEIRRRYFTRTRSSRYQPGDTRGWQL
                     SEARRIIAADPDWQRHITRCLYRPFDWRYVFWHPAMIDWPRTEVTRHLLGSQEGGTKR
                     RRDGETSECP"
     gene            complement(1936..>2159)
                     /locus_tag="VFV87_15095"
     CDS             complement(1936..>2159)
                     /locus_tag="VFV87_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5105144.1"
                     /translation="AGGAGRAGGVSPLSSSDLVQLTGRTHDDRIVVFDGNPRLIGEII
                     PVAIYDVSPHTLFGAVVTEHVGPGLQLIG"
BASE COUNT          381 a          638 c          748 g          392 t
ORIGIN      
        1 ggacactcac ttgtctctcc gtctctcctt ctcttcgtcc ctccctcttg gcttcccagc
       61 agatgccgcg ttacctcggt tcgcggccag tcgatcatcg ccgggtgcca gaagacatag
      121 cgccagtcga aggggcgata gaggcagcgg gtaatgtgcc gctgccagtc gggatcggcg
      181 gcgatgatcc gccgggcttc ggagagctgc cagccgcggg tgtcgcccgg ttgatagcgg
      241 ctggagcggg tgcgggtgaa atagcggcgg cggatttcgt cgtcgggaat ggccaggtcg
      301 cggaactcgg cgatccgccg ctgcagctcc tcctcgtgaa aggcgaccac gaagtggtcg
      361 cgggcggtca cgggggccgg cgtgttcacc ggcatcaatt ccgtgagcgg ccaaccggcg
      421 tcgtactcgg gatgaacggt cttgcgtgtg ggaacaaatc gccaatccgg cgcggcgggc
      481 cgaatcgtaa cccgaagcgt aagcgaggga gtgccgagcg cagcgagttt ctcatcgcgt
      541 gagccccaga gttcggcgta ttcaatgcgg ggaatgactc cctcgctgac gcttcgggtt
      601 ggtgtcactc cctcgcttac gcttcgggtt aggatgctga ttgccgtgcc ttgatcgagg
      661 ccgaagacgt tctcatcgcg gctgccgtcg ggagattgct cccccttctt acggctgccg
      721 tgcagatcga cgatctgaat gtgcggaaag gcgcggagca actcgctgcg catgaggcgg
      781 aacgtggcgt tgtcgagata gccgtggttg gtgacgaagc cgacgatgcc ttggcccgct
      841 tgctcgacgt gccattgagc cagtcggata aacttcacgt agtcgtcgtg gagccaggtt
      901 tttcgctcgc ccagcgaccg gccgttggcc cgcacatagc cgcagactac gtcatcaccg
      961 cggacgaggc gggcgatcca ggggttggtg ttggtcgaga ggctggagaa gggcgggttg
     1021 ccgacgacta cgggaattgc agatttcaaa ttgcaaatct caaattgcaa attgggctcg
     1081 agggcgtcgc cgatgcggat gttgatgctc cctggctgtc ggaagctgta gccggtcgcg
     1141 gccagcttga gggcgatgtt gagtttggcc aggaaggcgg cggcggggag gatttcgatt
     1201 ccgatcagcc gcggcaggat cttgggcacc accgcgttcc agtcgtcgcc aagctggcag
     1261 tggaggcgct cgataaccgc caggaggaag acgccagttc cgcaggccgg atcgagaacg
     1321 catggcgagc gggaggcgtc agcctcccgg taaggcggcc cggcggcggc tgccagaccc
     1381 tgtgcaagac cgaactcgcc gcgcagccgc tcatcgacct gacgcacaat gaagtcggcg
     1441 atcggcgccg gcgtgaagaa aacaccacgc tgtttgcgcg tgcagcgctg gtaggcgtgg
     1501 aggaaagtct catagaagtg ggcggacgaa ctagcgggaa tgtcgagtac cgagtactga
     1561 gtccttagta ctgagtactc ggtacgaatc tcctggagag cgcagcgttc ccagtctgac
     1621 gcctgcacgt ggaaccaccg cgtggccgag ttccattcct gcggctttga ggcgacgatc
     1681 accccgccgc aagtcgccgc ttgcgcgatc acattggctg cctcgtcaac tgagacttct
     1741 cggccaaggt cagcggcgag cgaatcacgg gcggattgca gctgctgatc gccggacagc
     1801 agccgcactt gcaattcgtc gtggagctgc accgcatgag cggccaaggc gcgtgccagg
     1861 ctgcgaatgg agcgcggctt cgtgctggcg gtagtgggca cagcgatggc aggcgagacg
     1921 cctaccccac gagtttcagc cgatgagctg caggccgggg ccgacgtgct cggtgacgac
     1981 ggcgccgaag agcgtgtgcg ggctgacgtc gtaaatcgcc acgggaataa tctcgccgat
     2041 cagccgcgga ttgccgtcga agacgacgat ccgatcgtcg tgcgtgcggc cggtgagctg
     2101 gacgaggtcg ctactgctca gggggctcac gccccccgct cgcccagcgc cccccgctc
//
LOCUS       DASUGB010000788         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1616060, whole genome shotgun sequence.
ACCESSION   DASUGB010000788 DASUGB010000000
VERSION     DASUGB010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616060"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFV87_15100"
     CDS             complement(<1..517)
                     /locus_tag="VFV87_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5105145.1"
                     /translation="MYRRLRWERFRGAFTLVELLVVIAIIGILVALLLPAVQAAREVS
                     RRTSCANNLKQIGLAAHNFHDIHERFPPGYLGPLPHDDFNNFPNQQYLGALAYLLPHM
                     EQQQLHSLIRTNQEYQVLGEPGWWNDPSTASAAMVKVKPFLCPSTNAHVSPSGVAATL
                     NVYQSSTALEFQ"
     gene            867..2432
                     /locus_tag="VFV87_15105"
     CDS             867..2432
                     /locus_tag="VFV87_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5105146.1"
                     /translation="MSATPITAADATTEPDLSGRRLGDYQMLRRLGRGGMAEVYLAEQ
                     ISLRRQVAFKVLKSSLAGDTAYVRRFHHEAQAAANLVHANIVQIHEVGCIDGIHFIAQ
                     EYVPGQNLRQLLARLTKGVDARQAANIIRQVAAALHKAAEQNIIHRDIKPENIMISTS
                     GEVKVADFGLARIAQGGEALNLTQVGMTMGTPLYMSPEQVEGKPVDPRSDIYSLGVTC
                     YHMLAGRPPFDGDTALAVALQHLRNEPKRLEAIRPDLPEGLCRVVHKMLAKKSEERYQ
                     RAVDLLRDLKGLQIPGLDEDWVSDLPGFTSADLALGAAGRLAATQQLNRVLQSQIKGR
                     PVSRPLPVIVAAIAALLLAGLALGAAIAWINRPQPLLELSESERPKIKKFESAKEQYS
                     YAQMASLDQKEDAWKAVAEYHPPEESPDNMRYSRLAMKGLAAFYLNDNRVPEALPLYE
                     KLAKAESTEREFQLIGLVGQAVCYDRLGNEIKAKESLAQVRPYLDSLPLNLGSTLRDG
                     LNKLMETYPQEGE"
BASE COUNT          539 a          814 c          732 g          481 t
ORIGIN      
        1 tctgaaactc cagcgccgtg ctgctttggt atacgttcag ggtcgccgcg acgcccgagg
       61 gtgacacatg cgcattcgtg gagggacaaa gaaaaggttt aaccttcacc atcgctgcac
      121 tagcggtcga aggatcattc caccaacccg gctcgccgag aacttgatat tcctgattgg
      181 tccgaatgag gctgtgtagt tgctgctgct ccatatgcgg caagaggtag gcaagcgcgc
      241 cgagatactg ctgattgggg aagttgttga aatcatcgtg gggaagcggc cccaggtatc
      301 ccggcggaaa cctttcgtgg atgtcatgga agttgtgcgc cgcaagaccg atctgcttga
      361 ggttattggc gcaagaggtc cgccgcgaaa cttcacgcgc ggcttgaacc gccggcagaa
      421 gcagcgctac aagaattcca atgatcgcaa ttaccaccag caattccacg agcgtaaatg
      481 cgccgcggaa ccgttcccac cgcagtcgtc gatacatgac ggattcctct cgctttagct
      541 cgagcgaaga agaactccca gcaggaagtt gcgaactccc ccccggattc tagcccagca
      601 cttcgacaat tcaatcagtt ttggacggat cgatacgcga tcatccaaac agccgcattg
      661 cgaccgcaat aatcgctgcc cccggccatt atactcgaag gtgcgttttt gcctctcgtt
      721 tggcgcttgc ggttgtcagt tgtcccgatc cgtccgatac taagattctc gacctgtcca
      781 atggctgtgt tggccccact ttcagtgacc gacatcgtcg aaatcagctc cgccctagac
      841 ggactcggat cacttcgtcc cacctgatgt ccgccacccc gatcactgcc gcggatgcga
      901 cgaccgagcc cgatttgtcg ggccgcaggc tgggcgatta tcagatgctc cgccgcctgg
      961 gccgcggcgg catggcggaa gtctatctgg ccgagcaaat ctcgctccgc cgccaggtcg
     1021 cattcaaagt actcaaatcg agtctggccg gcgacaccgc ctacgtccgc cgcttccacc
     1081 acgaagccca ggccgccgcc aacctggtgc acgccaacat cgtccagatc cacgaagttg
     1141 gctgcatcga cggcatccat ttcatcgccc aggagtatgt gccgggccaa aaccttcgcc
     1201 aactccttgc ccggctgacg aagggagtcg atgcccgcca ggccgccaac atcatccgcc
     1261 aggtcgccgc cgccctgcac aaggcggccg agcaaaacat catccatcgc gacatcaagc
     1321 ccgagaacat catgatctcg acctcggggg aggtgaaagt tgccgatttc ggcctggctc
     1381 gaatcgccca gggaggcgaa gccctcaatc tcacgcaggt gggaatgacg atgggcacgc
     1441 cgctctacat gagtcccgag caggtcgagg ggaagccggt cgatcctcgg agcgacatct
     1501 attcgctggg ggtcacttgt tatcacatgc tcgccggccg gccgccgttc gacggcgaca
     1561 ctgcgctcgc cgtcgccctc cagcatctga ggaacgagcc caagcggctg gaagcgatcc
     1621 gccccgatct ccccgagggc ctctgtcgcg tcgtccacaa gatgcttgcc aagaagtccg
     1681 aggagcgcta tcaacgagcg gtcgatctgt tgcgcgacct caagggactg cagattcccg
     1741 ggctcgacga agattgggtc agcgatttac ccggttttac aagcgccgat ttggccctgg
     1801 gagcggcggg tcgcctggca gcgacgcagc agctcaaccg cgtgcttcag agccaaatca
     1861 aaggccgtcc cgtttcgcgt ccgctgccgg taatcgtggc cgccatcgca gccctcctct
     1921 tggctgggtt ggctctgggg gccgcaatcg cctggatcaa tcggccgcag cccttgctgg
     1981 agctatccga aagcgagcgg cccaagatca agaaattcga atcggccaaa gagcaatact
     2041 cgtacgctca gatggcatct ctcgaccaga aggaagatgc ttggaaagcg gtcgccgagt
     2101 atcatccgcc agaagagagc cctgacaaca tgcgctattc gcgcctcgct atgaagggcc
     2161 tggcggcgtt ctatctgaac gacaaccgtg ttccggaagc tctgccgctg tatgagaagc
     2221 ttgccaaggc cgaatcgacg gagcgagaat tccagctcat tggtctggtt ggtcaggcgg
     2281 tctgctacga ccggcttggc aacgaaatca aggcgaagga atcgctcgcc caggtcaggc
     2341 cttacctcga cagcctgccg ctcaacctgg gcagcaccct tcgcgacgga ctcaacaaat
     2401 tgatggaaac ctatccgcag gagggcgaat gagccctgcg gtcgctgacc taggaaccct
     2461 ctcccatgcc ctgctccctt cgcggacgcg tcgcactgat caccggcaat agcacgggcc
     2521 tcggcaaagc catcggcctg gccctcggca aggcgggagc gaaagt
//
LOCUS       DASUGB010000789         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1892308, whole genome shotgun sequence.
ACCESSION   DASUGB010000789 DASUGB010000000
VERSION     DASUGB010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892308"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(672..2099)
                     /locus_tag="VFV87_15110"
     CDS             complement(672..2099)
                     /locus_tag="VFV87_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105147.1"
                     /translation="MFFLRTFQLGLKSLLLHPMRSLLTVLGIFIGVASVIWLLAIGEG
                     ISQEAQRQIEGLGADNIIVRSVKPPAEATAGFTGPVPYGLKREEYDLLVRTIPTIRSA
                     LPIREVTRTFSYSGRDVDGRLVGCTPEYFDVTRLEVDRGRRLTDADIDERQNYCVLAS
                     EIAKLLFPYEDPIGRRVYLSESKDFYQVVGILKYRTPTAAIGGSLDAQDFGSDVYIPI
                     STMRQRVGDFVITRRGSTFSTEIVELNQITIRVNTDGAPVPADVVRATADLVRQSLGV
                     RDESKRNKPVISAATNPQLAAMEAAEAASPGRRDVAVIVPEELLEQARVTKFMFMVFM
                     GLIAAISLLVGGIGIMNIMLATVTERTREIGIRRALGATRMHIIMQFLVETISLSVVG
                     GLTGILVGLLCPVVIVALRELAFVYAADMMASLPDVVRKVEPTIVPLSLPLAFGISVV
                     VGVVFGIYPAYRAAQMDPIEALRHE"
     gene            complement(2109..2882)
                     /locus_tag="VFV87_15115"
     CDS             complement(2109..2882)
                     /locus_tag="VFV87_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5105148.1"
                     /translation="MTVAARLVEIKKDYVLKSETVRALRGVTFEVPEGDYVAIMGPSG
                     SGKSTLLNLLGCLDRPTSGQFYLGPDDVSRMNDNQLSWIRATRIGFIFQSYNLIQQLN
                     VLENIGVPLHYQGKSGRERCRQLAEMVGLGERLDHRPTQLSGGQQQRVAIARSMVNDP
                     YFMLADEATGNLDSVTSEEILHLFEKLNDAGRTIIMVTHEDDVAAHAKRIIRLRDGLL
                     QSDRLNDHRVAARRNVQVPGADLVAQTADSWSPPLTTDN"
     gene            complement(2879..>4610)
                     /locus_tag="VFV87_15120"
     CDS             complement(2879..>4610)
                     /locus_tag="VFV87_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5105149.1"
                     /translation="GPQVITNVVSRAPFEFVVIEQGEVESANATELRCEVKSRGTGGG
                     GITILEVVPEGTMVQPGDVIVRLDSSALEQENVTQQIKCNSQESLVIQAKNTLEAAKI
                     ARTEYLEGTYKNEEALILNEIFVAEQALRTAETGLASAQRLAARNIITALQLEGAQFN
                     VDKARNDVDAAQNKLNALRKYTQAKMLKQFDSDIATADAKVKAEESSYQLELDKLKEI
                     EDQITKCVISAPNAGQVVYCNRYNSGRSGSTAEFVVEAGSTVREQQPIIRLPRSNEMK
                     VKATVNEARVTLVRPGLPVSIRVDALKDVSIMGEVEKVNQYAEPGSWSGGNVKKYATF
                     VKINDPPAELRNGMNAEVRIYVERQSDALQVPVQALAEHKGHFFALVKSGGKYETREV
                     SINSTNDKVAVIKDGLAEKDEVVMNPRGQPAMFHLPSLPDETTAQIADIPRAAGTPAI
                     MTASLSGNPGIPGPGAPGEGGPPGEGAGGERPKGKGKGKAGAFTPANIVDRALEESDT
                     DKDGKISMAELASMEDRRKQNFQGGDANGDGFLDRAEMMNAATAAMAARARERMEGGG
                     GGGPPAGGGQ"
BASE COUNT          802 a         1470 c         1453 g          885 t
ORIGIN      
        1 acaggtcgtt gacggcgagc ggcgatgcgc cggacaggac catgcggtcc tccaggcgct
       61 cgacgcggag aaaattccgc cgtgacgtat cgcggcggcg gctcttttgc ttccgagaaa
      121 ggtacgtgct acgcataggg aaatcagctc ctgcgaagga aaggcggcgc gccggccgcc
      181 ggcgagtgca aagcgacaga ggccggccca cgcggaccgg attgaccagt gagaacgggc
      241 agggatcggc gagccgcaat cggcccgcac tacgcggaaa gatgccagcg ccgctggccg
      301 cgaatcgcgg cagccgggcc ggaaggcgtc ccagcgccca agggtctcat cgcttctccc
      361 tgttccctga gcgatgagcg gcgggcgttc gcgccatcgc gaaaagccgc gccaagttgc
      421 aggcgtttgc ttgatcgggg caatcgggcg cacctgcgcg ccaaccaaac cagtcgttgc
      481 aacgtcaccg cccccgcggc tatccggacg agccccgcgg agcgatggtg ttaatctacg
      541 aaccagattt cccgttgcaa caagctagcc tgcagaatct cagaaatgat tcagcgcagc
      601 gactgcgcgc cggcgcaggc cgcgccagcg gcggcagcgc gaatgggtgg gtgaaaccgc
      661 cgaatgaagg gttactcgtg ccgcagggcc tcaatcgggt ccatctgcgc ggcgcggtaa
      721 gcgggataaa tgccgaacac cacgccgacg acgactgaaa tgccgaaggc cagcggcagc
      781 gagaggggca cgatcgtcgg ctcgaccttg cgaacgacgt cgggcaggct ggccatcata
      841 tcggcggcat agacgaaggc cagctcgcgc aaggcgacga tcacgaccgg gcagagcagg
      901 ccgacgagaa tgcccgtcaa tccgccgaca acggacagcg agatcgtttc gacgaggaac
      961 tgcatgatga tgtgcatccg cgtcgcgccg agggcccggc ggatgccgat ttcgcgggtt
     1021 cgttccgtga ccgtggccag catgatattc atgatgccga tgccgccgac caggagcgaa
     1081 atcgcggcaa tgaggcccat gaacaccatg aacatgaact tcgtgacgcg ggcctgttcg
     1141 agcagctcct ccggcacgat caccgccaca tcgcggcggc cgggcgacgc ggcttcggcg
     1201 gcttccatcg cggcgagctg tgggttggtc gcagccgaga tgacgggctt gttgcgtttc
     1261 gactcgtcgc ggaccccgag cgactggcgg acgaggtcag ccgtcgcccg aacgacgtcg
     1321 gcgggcaccg gtgcgccgtc ggtattgaca cgaatcgtaa tctgattcag ctccacgatt
     1381 tcggtgctga aagtgctgcc gcggcgggtg attacaaaat cgccgacgcg ctgccgcatg
     1441 gtggagatgg gaatatagac gtcgctgccg aaatcctggg cgtcgagcga gccgccgatg
     1501 gccgcggtcg gcgtgcggta ctttagaatt ccgacgacct ggtaaaagtc cttgctctcg
     1561 gagagataga cgcggcggcc gatcgggtct tcgtacggaa agagaagctt ggcgatttca
     1621 ctggccagga cgcagtagtt ctgccgctcg tcgatgtcgg cgtccgtcag ccggcggcca
     1681 cgatcgacct ccaggcgcgt tacgtcgaaa tactccggcg tgcatcctac gaggcggccg
     1741 tccacgtcgc gcccgctgta gctgaaggtg cgggtgacct cgcggatcgg cagggcggag
     1801 cgaatcgtcg gaatcgtgcg cacgagcagg tcgtattctt cgcgtttcag accgtacggg
     1861 accggccccg tgaatccggc cgtggcctcg gcaggcggct tgactgagcg gacgatgatg
     1921 ttgtcggcgc ccagaccctc aatctgccgc tgggcttcct ggctgatccc ttcgccgatc
     1981 gccaggagcc agatcacgct cgccacgccg ataaaaatgc ccaggacggt cagcagcgac
     2041 cgcatggggt gcaagagcag gctcttgagg ccgagttgga acgtgcgcag aaaaaacacg
     2101 ggctgttgtc agttgtccgt tgtcagtggg ggactccacg agtcggcggt ttgggcgacg
     2161 aggtcggcgc ctggaacttg cacgttccgg cgggccgcaa cacggtggtc attgaggcgg
     2221 tcgctttgca acaggccatc gcggaggcga atgattcgct tggcgtgcgc cgcaacgtcg
     2281 tcttcatgtg tgaccatgat aattgtgcgg ccggcgtcat taagcttctc gaacaagtgc
     2341 aggatttcct cgctggtgac cgagtcgaga ttgcccgtcg cttcgtccgc cagcatgaag
     2401 tacgggtcgt tcaccatgct gcgagcgatc gccacgcgct gctgttgacc gccggagagc
     2461 tgcgtcggcc ggtggtcgag gcgctcgccc aggcccacca tctcggcgag ttggcggcag
     2521 cgctcgcggc cactcttgcc ctggtagtgc aagggaacgc cgatgttctc cagcacattg
     2581 agctgctgga tcaagttgta cgactggaag atgaacccaa tccgggtagc gcgaatccag
     2641 gaaagctggt tgtcgttcat ccgcgaaaca tcgtctggcc ccaagtagaa ctggcccgac
     2701 gtaggccggt cgagacaccc cagcaggttc aagagcgtgc tcttgcccga gcccgacggc
     2761 cccatgatcg cgacgtagtc cccctcggga acttcaaacg tcacgccccg cagcgcccgg
     2821 accgtttcac ttttgaggac gtagtccttt ttgatttcga cgagcctggc cgcgaccgtc
     2881 attggccccc tccggctggt ggcccgccac cgccgccgcc ttccattctt tcgcgggcac
     2941 gcgcggccat ggctgctgtg gcggcattca tcatttcggc ccggtccaaa aagccgtcgc
     3001 cgttggcatc gccgccttgg aaattctgtt tgcgtcggtc ctccatcgag gccaactcag
     3061 ccatcgaaat cttgccgtcc ttgtcggtgt cgctttcttc cagggcgcga tcgacgatat
     3121 tcgccggcgt gaatgcgccc gccttcccct tgcccttgcc tttgggccgc tccccgccgg
     3181 cgccctcgcc gggcgggcca ccctcgcctg gtgctcccgg gcctgggatg ccaggattac
     3241 cgctaagcga ggcagtcatg atcgctggag tgcctgccgc gcggggaata tccgcaatct
     3301 gggcggtcgt ctcatccggc aggctcggca ggtgaaacat ggccggctgg ccgcgaggat
     3361 tcatcacgac ttcgtccttc tccgccagtc catccttgat cacagcgact ttgtcgttcg
     3421 tcgaattgat cgacacctcg cgcgtttcgt actttccacc gctcttgacc agggcgaaaa
     3481 aatgcccctt gtgctccgcc agcgcctgga ctggaacctg caaggcatcg gactgccgct
     3541 cgacgtaaat ccgcacttcg gcgttcatgc cgttgcgcag ctcagccgga ggatcgttga
     3601 tcttgacgaa cgtcgcgtac ttcttcacgt tgccgcccga ccagcttccg ggctcagcat
     3661 actggttgac cttctccacc tcgcccatga tcgacacgtc cttgagggcg tcgacgcgga
     3721 tgctgacggg caagccggga cggaccaacg tgacgcgggc ctcgttgacc gtcgccttca
     3781 ccttcatctc gttcgagcgc ggcaaacgga tgatcggctg ctgctcgcgg acggtggacc
     3841 ccgcctcgac gacgaattcc gccgtcgaac cgctacgccc ggagttgtac cggttgcaat
     3901 acaccacctg gccggcattg ggcgcgctga tcacgcactt ggtgatctga tcctcaattt
     3961 ccttgagctt gtcgagctcg agctgatagc tactttcctc cgctttgacc ttcgcgtcgg
     4021 ccgtggcgat gtcggaatcg aactgcttga gcatcttggc ctgggtgtac ttgcggaggg
     4081 cgttcagctt gttctgggcc gcgtccacgt cgttacgggc cttatcgacg ttgaactggg
     4141 cgccttccaa ctgcagcgcc gtgatgatgt tccgcgcggc cagccgctgg gcggaggcca
     4201 ggcctgtttc tgccgtgcga agcgcctgct cggccacgaa gatctcattt aagatcaagg
     4261 cttcttcgtt cttgtacgtc ccttccaagt actcggtgcg ggcgatcttc gccgcttcga
     4321 gcgtgttctt cgcctggatg accagcgact cctggctgtt gcacttgatt tgctgagtga
     4381 cattttcctg ctccagggcg ctggaatcga gccgcacgat cacatcgccc ggctggacca
     4441 tcgtcccttc gggaacgacc tccagaatcg tgattccgcc gcctcccgtg ccgcgcgatt
     4501 tcacttcgca ccgcagttcc gtcgcattgg cgctttccac ttcgccttgc tcgatcacga
     4561 cgaactcaaa cggcgcgcgg ctgacgacat tcgtaatgac ctgcggcccc
//
LOCUS       DASUGB010000790         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1336952, whole genome shotgun sequence.
ACCESSION   DASUGB010000790 DASUGB010000000
VERSION     DASUGB010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336952"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFV87_15125"
     CDS             <1..259
                     /locus_tag="VFV87_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105150.1"
                     /translation="RRSVFVELTPQGEAFVAKAPSPLQSALVDRLHKLSPKDQQRLAD
                     SLEEVVSLMQAESLDEVPVLASSRFAGSRRPRGREAAADLQ"
     gene            315..>2205
                     /locus_tag="VFV87_15130"
     CDS             315..>2205
                     /locus_tag="VFV87_15130"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX5105151.1"
                     /translation="MKHALSQLETGKCRVLIESVSPQVDQGRFAAKRILGDAVDVRAR
                     IFADGHDLLSSRIQYRHHSESSWREAELQLQVNDWWHGSFVVERLGRYFFTTLAWIDR
                     FGTWRRDLRKRVDAGQDVAVDLLVGAGIVREHLPQLPLADQPALEKLAQQLEAASDRD
                     AQLAAAFSPVLADLMSRHADRRFATKYDRELPLEVDRPLARFSTWYELFPRSCAPEAG
                     RHGALADVEARLPYVAEMGFDILYLPPIHPIGRSFRKGKNNAVTAAEGDVGSPWAIGA
                     AEGGHKAIHPDLGTLDDFRRLVAKAREHNLEIALDIAYQCAPDHPYVSAHPNWFRQRP
                     DGTIQYAENPPKKYQDIYPFDFESEDWQNLWEELKSVLLFWGKEGVRVFRVDNPHTKS
                     LLFWEWAIAEVKAVYPETIFLSEAFTRPAVMYRLAKAGFTQSYTYFTWRNTKHEIESY
                     FTELTKEPVREFFRPNLWPNTPDILNEYLQFGGRGAFLARYVLAATLGASCGIYGPTF
                     ELCVGQPREPGSEEYLDSEKYQIRHWDWNAQYSLKAFITRVNQIRRENPALHGDWSLQ
                     FHPTDNEQLICYSKQTADGSNIVIAVVNLDPQHTQSGWIELPLEEFGVETDRPFQAHD
                     LLGEGR"
BASE COUNT          419 a          681 c          700 g          405 t
ORIGIN      
        1 tcgccgctcg gtgtttgtgg aactgacgcc gcaaggcgag gccttcgtcg ctaaggcccc
       61 ctcgccgctg caatcggccc tggtggaccg cctgcacaag ctcagcccta aggatcagca
      121 gcggctggcg gattcgctgg aagaagttgt aagcttgatg caggctgaga gcctcgacga
      181 agtgccggtg ctggcgtcgt ctcggtttgc cggatcgcgc cgcccgcggg ggcgagaggc
      241 ggcagcggat ttgcagtgat ccgagccgta gtatttgagc ggcagcgtgg aatcctcgca
      301 ctcggaagtc tgcgatgaag catgcgcttt ctcaactcga aaccggcaag tgccgcgtcc
      361 tgatcgagag cgtttcgcca caggtcgacc aggggcgctt tgcggccaag cgcattctgg
      421 gcgatgccgt cgatgtgcgg gcccgcatct ttgccgacgg gcacgatctc ctcagcagcc
      481 gcatccagta tcggcatcat tccgaaagct catggcgcga agccgagctt cagttacagg
      541 taaatgattg gtggcacggc agctttgttg tggagcggct ggggcggtac ttcttcacga
      601 ccctagcctg gatcgatcgc tttgggacgt ggcggcgaga tctgcggaag cgcgtcgatg
      661 cggggcagga tgtggccgtt gatctgctgg ttggcgcggg catcgtgcgc gagcacctgc
      721 cgcaattgcc ccttgcggat cagccggcgc ttgaaaaact ggcccagcag ctcgaagccg
      781 cgagcgatcg cgatgcacag cttgcggccg cattttcgcc ggtcctggct gacctgatga
      841 gccgccacgc cgatcggcgc tttgcgacga aatacgaccg cgagttgccg ctggaggtcg
      901 atcgcccgct ggcccggttc agcacctggt atgagctgtt cccgcgctcc tgcgcaccgg
      961 aagcgggccg ccatggcgcg ctggcggatg tggaagccag gctcccctac gtcgccgaga
     1021 tgggattcga cattctctat ctaccgccga tccatccgat cggccgcagc tttcgcaaag
     1081 gaaagaacaa cgcggtgacc gccgcggaag gcgacgtcgg cagcccgtgg gcgatcggcg
     1141 cagccgaagg cgggcacaag gcgattcatc cggatctcgg gacgctggac gattttcgcc
     1201 gactggtcgc caaggcccgc gagcacaacc tggagatcgc cctcgatatc gcctaccagt
     1261 gcgctccgga ccatccgtac gtctccgccc atccgaactg gttccgccag cgcccggacg
     1321 gcacgattca atatgccgag aacccgccga agaaatacca ggatatttat cccttcgact
     1381 tcgaatcgga agactggcaa aacctgtggg aagagctgaa gagcgtgctg ctgttctggg
     1441 gcaaggaggg cgtgcgggtc tttcgcgtcg ataatccgca caccaagtcg ctcctgttct
     1501 gggaatgggc gatcgccgaa gtcaaagcgg tctatcccga gacgatcttc ctctccgaag
     1561 ccttcacccg gccggcggtg atgtaccgcc tggccaaagc cggcttcacg cagtcgtaca
     1621 cgtatttcac gtggcggaac acgaagcacg agatcgagtc atacttcacc gagctgacga
     1681 aggagccggt ccgcgagttc ttccgcccca atttgtggcc gaacacaccg gacatcctca
     1741 acgagtacct gcagttcggc ggacgcggcg cttttctggc ccgctacgtc ctggcggcga
     1801 cgctcggcgc ctcgtgcggc atctacggac cgacgttcga gctgtgcgtc ggccagccgc
     1861 gggagcccgg cagcgaagag tatctcgatt cggagaaata ccagatccgg cactgggact
     1921 ggaacgccca gtacagccta aaagccttca tcacgcgcgt gaatcaaatc cgccgcgaga
     1981 acccggcgct gcacggcgat tggagcctgc aattccaccc gaccgacaac gagcagctca
     2041 tttgctatag caaacaaacg gccgatgggt ccaatattgt gatcgcggtg gtcaatctcg
     2101 atccgcaaca tacgcagtcg ggctggattg aattgccgct ggaggaattt ggcgtggaga
     2161 ccgaccgccc gttccaggcc cacgatttgc tgggcgaagg gcggt
//
LOCUS       DASUGB010000791         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2032411, whole genome shotgun sequence.
ACCESSION   DASUGB010000791 DASUGB010000000
VERSION     DASUGB010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032411"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2445)
                     /locus_tag="VFV87_15135"
     CDS             complement(<1..2445)
                     /locus_tag="VFV87_15135"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105152.1"
                     /translation="MACVAVASAETKGRLATYDKDGEQYFALSLSPGVPAAANQANEV
                     VILFDTSASQAGNYRTDALSALDSLLGMLQATDRVHLMAVDMKAVPMMTGFAAPGSPE
                     MTAAVTKLRARAPLGATDLESGLASAAASFSEGDAARTVIYIGDGMSKAKIFSDASYA
                     RLVGDLRNGHVAVSSLVIGSERNAHLMATLANHTGGVVRVDSDAANAATTAGQALARS
                     VHAGVLWPSEVTLPANVTASYPALVPPLRTDRDSVLIGTMSNSEAGEVKISGTLDGKP
                     VDLTWNVTPEKSSDDFSFLPQLVSLASKDSGLSLPTVGSEGLREAAQVTIASADQLSK
                     LGHEALASGNFAGAAKVADAALARDPANPAALAIKDAASRRTAPATAARAAAASEPEL
                     RLASGDDRPSGSLLAEVLAEEPGFLSSVESEAAVLAGKIQAEVENGLNEARKTMSESP
                     EIAEQNLKALLEYVERVPELNAEVRAQLRQQIENAIREANRRKIEVAEIIRAAEERKA
                     QGQELQRINDDLLAEQLKLKQIMDRFDALMDEGRYDIADDEIAPEVERLVPDSIIRES
                     VTTGARLQRNYRDAAAVWEQREKNFVLTLYQVERSFIPFPDEPPIVYMPADQWEDLTL
                     RRAKYKAVDLGKQGSSEERIFKALNDQAVMEFIETPLNDAINILKDTHQIPIELDVKR
                     LEEAGVNIDTPVTKNLRGITLRSALRLLLNDLELTYVVRDEVLLITTPEEAESQLITK
                     VYPVGDLVVPIGINSNMFGLGGFGGGGFGGGGGMMGGGGGGFFAVEDELSLGTKKPAA
                     APAKPAPAVEEPQVRRP"
BASE COUNT          405 a          806 c          761 g          496 t
ORIGIN      
        1 tgggcggcgc acttggggtt cttccaccgc tggggccggt ttggccggcg ctgctgcggg
       61 ctttttcgtg ccgagcgaga gctcatcctc gacggcgaag aatccgccgc cgccgccgcc
      121 catcatgccg ccgccgccgc cgaagccgcc gccgccgaag ccgcccaggc cgaacatgtt
      181 gctgttgatg ccgatcggaa ccaccaggtc gccgactgga tacaccttcg taatgagctg
      241 gctttcggcc tcttcaggcg tggtgatcag gaggacttca tcccggacga cgtaggtcag
      301 ctccaggtcg ttcaagagca gacgcagggc cgagcggagc gtaatgccgc ggaggttctt
      361 ggtgacgggc gtatcaatat tcacgcctgc ctcttccagt cgcttcacat ccagctcaat
      421 tggaatctga tgcgtgtctt tgaggatgtt gatcgcgtca ttgagaggcg tttctatgaa
      481 ttccatcacc gcctgatcgt tgagggcctt gaaaattcgc tcttcggagc tcccctgctt
      541 gcccagatcg accgccttgt atttggctcg ccgcagcgtc aggtcttccc attgatcggc
      601 cggcatatag acgatcggcg gctcgtccgg gaagggaata aatgagcgtt cgacctggta
      661 caaggtcagc acgaagttct tttcgcgctg ttcccacacc gctgcagcat cacggtaatt
      721 gcgctgcaag cgggcgccgg ttgtgaccga ctccctgatg atggagtcgg gaacgaggcg
      781 ctcgacctcc ggagcaatct cgtcgtcggc gatatcgtag cggccttcgt ccatcagagc
      841 gtcgaaacgg tccatgatct gcttcagctt gagctgttcg gcgagaaggt cgtcgttgat
      901 ccgctggagc tcctggccct gtgcctttcg ctcttccgct gctcgaatga tctccgcgac
      961 ttcgatcttc cgacggttgg cctcgcgaat cgcgttctcg atctgctggc gaagctgggc
     1021 gcggacctcg gcattcagct ccggcacgcg ttccacgtat tccagaagcg ccttcagatt
     1081 ctgttcggca atctcggggc tttcggacat cgtcttccgg gcctcgttga gaccgttttc
     1141 aacctccgcc tgaatcttgc cggccagaac cgccgcttcg ctttccaccg agctcaagaa
     1201 tcccggctct tcggccagaa cttccgcgag caagctgccc gaggggcgat cgtcgccgct
     1261 cgctagccgc aattccggtt cgctcgcagc cgcagctcga gcggctgtcg ccggagcggt
     1321 gcgcctgctg gcggcgtctt tgatggccag ggcggctgga ttggcaggat cgcgcgccag
     1381 agccgcatcg gcgaccttgg ctgcgccggc gaagttcccg ctggccagcg cttcgtggcc
     1441 gagcttggag agctgatcgg cactagcgat cgtcacctgg gccgcttccc gcagaccctc
     1501 cgagcccacc gtcggcagcg acaggccgct gtctttggag gccaggctca cgagctgcgg
     1561 caggaatgag aaatcgtcgc tcgatttctc aggcgtcacg ttccaagtca aatcgaccgg
     1621 cttgccgtcc agcgtgccgc tgatcttcac ttcgcccgct tcgctattgg acatggtgcc
     1681 gatcagcacc gaatcccggt cggtccgcag cggcggcaca agcgccgggt acgaagccgt
     1741 cacgttcgcc ggcagcgtca cttcgctcgg ccagagcaca ccggcgtgaa cgctgcgggc
     1801 cagcgcctga cctgcggttg tggccgcgtt ggcagcgtcg gaatcgacgc gcacgacgcc
     1861 gcccgtgtga ttggccagcg tcgccatcag gtgggcgttt cgctcgctgc cgatgaccag
     1921 gctggatacg gccacgtgtc cgttgcgcaa atcaccgacg agacgggcat acgacgcatc
     1981 gctgaaaatc ttggccttgc tcatgccgtc gccgatatag atcaccgtac gggcggcatc
     2041 accttcgctg aagctcgccg cggcagacgc caggcccgat tccaggtccg tcgcgcccag
     2101 aggcgctcgg gcccgcagct tggtcacagc cgctgtcatt tccgggctgc ccggagcggc
     2161 aaagcccgtc atcatcggca cggccttcat atcgaccgcc atcaaatgaa cgcggtcggt
     2221 cgcctgcagc atcccgagca gcgaatcgag cgccgacagt gcgtcggtgc ggtagttgcc
     2281 cgcctggctg gccgaggtgt cgaacaggat taccacttcg ttcgcctgat tggcagcggc
     2341 cggtacgcca ggcgacaagc tcaaggcgaa gtattgctcg ccgtctttat cgtaggtcgc
     2401 caggcggcct ttcgtttcgg cagaagcgac cgcgacacag gccacggcca atacgggtgc
     2461 caaagcca
//
LOCUS       DASUGB010000792         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_921152, whole genome shotgun sequence.
ACCESSION   DASUGB010000792 DASUGB010000000
VERSION     DASUGB010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921152"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFV87_15140"
     CDS             <1..904
                     /locus_tag="VFV87_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5105153.1"
                     /translation="ALLMGVPGLAKTLLVSKLSETLTLKFSRIQFTPDLMPMDITGTE
                     ILEENRSTGNRERRFLEGPLFSNIILADEINRTPPKTQAALLEAMQERQVTVGRVRHP
                     LTDPFFVLATQNPIEQEGTYPLPEAQQDRFMFKVFVNYPNFNEEFEVARRTTAVQTEN
                     IQPALSGQEIIELQRTVREVPVSDHIIRYALSLVRQTRVGQNGVPDFVNDQIGWGAGP
                     RAVQFLILGGKARALLRGRTHVSTDDIQALAKPVLRHRLVVNFAAQSDGITADKIIDR
                     LIASTPTKEDELTSDARFKKIFAS"
     gene            873..1775
                     /locus_tag="VFV87_15145"
     CDS             873..1775
                     /locus_tag="VFV87_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5105154.1"
                     /translation="MPGSKRFLHPEAIKRISRLEVRARHIVEGFLSGMHRSPYFGQSV
                     EFLQHREYALGDDLRHVDWKVWARQDRLYVKQFEEETNLRSHLLVDVSNSMAYGSGPL
                     NKYEYACTLACSLAYLILRQQDGVGCITFDEKVRVTVPIRTKHNHIFSIVDSLAVQEP
                     KEKTDMYAILRQVAETAPRRGLMVLVSDLLADRPRLIKGLKLLRQRGHDVLVFHVMDD
                     EELDFPFSGPMRFEGLETPDFLNCNPRALRDGYLAALQAYLDDIRHACARNSCDYALV
                     RTGEPLDAALATFLSKRITVMNTY"
     gene            1959..4403
                     /locus_tag="VFV87_15150"
     CDS             1959..4403
                     /locus_tag="VFV87_15150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5105155.1"
                     /translation="MQFVHQILTWGFLLALVPLLIHLINMMRHRRVKWAAMEFLLASY
                     KKHRKWVWLKQLLLLLSRMAVVALIVAMLAQLKTRDQWLAIFGGRVTHHYVLVDDSYS
                     MSDRVAGATAMDAAKQVMAAIVGRATQEDSAQKLTLIRFSQARGAEERGQGTGDRGQG
                     TGDRGQGEEDRGQKTEDSVSQSAIRNPQSAIPADVADFNAEPIDSNFEVTLERKIRPF
                     EATQLALGPLDALSVVKQLLSQTKDETSIVYLLSDFRTKDWGSPTEYREALGQLRRSQ
                     AEVHLVNCAKSSEPNLGVISIEPADETRAAGVPLFVNVKVKNFGAKAASKVQLKLQSA
                     FYPADDVTASQPESLKGEVEDLATLLIESIGPGEVVDRRVQVYFPQPGKHVVEASLPE
                     DPVEADNHRWCVIDFPDGERVLVIDGSVDQQHAYFLEVAFRPLERSNTGIRPEVKPAS
                     FLRDATLETLNGYSAIYLLDVSRLDGRAADTLEAYVTGGGGVAIFAGPTMNTGYYNQA
                     LYKEGQGLLPAPLGLETDLAPALDPTEPDLELTSHPVFSFFTTETNPLIRGVKIDRYR
                     QVADQWKPGPESAVEIIGRTRDKSPLIIEKRLGQGEVLLFLTTLAPDWNDWAKNPSFV
                     VVLLKTQSYLATAKRLDDPRLVGTPVDVALEAAKYRPDMTFIVPGEKTGSRQKIDRRA
                     EPPSGSQADAATLVAALGRTPVAGKSRGETDRAGVYEAWPVTTRGEIDLKRWALNVEP
                     DEGDLTPITSTDLIAKLEPVKVNYHLADQYQQEDVASTGYNLSTLLLMGLIALLIGEQ
                     VLAYSASYHVTPGAVR"
     gene            4418..>5397
                     /locus_tag="VFV87_15155"
     CDS             4418..>5397
                     /locus_tag="VFV87_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105156.1"
                     /translation="MLAEASTNIQYKLARLQTLTEWWHWIALVAVSVAIVAFVVWMYI
                     RDSVELPRGLAITLCLLRLVAFFGILFYFFGLEKRAERKLVKNSRAILLIDTSQSMGL
                     RDSDSSNVPAAMSRVEHVAAELASGSMLEKLREKHDVIAYRFDQGENPVEIASYPRKP
                     PPEETAETTMSEQSRLNQIVDEARLLAYVAAGVLAVSVIAGLVYMAFGSRAVAGEPTS
                     WALLVSMTTLIAAVVILAVASLRSSEVGPLAVLGLREVKPLEEEQDETSTEEPAEIPT
                     VDWAAQLAPRGGETRIGDNLKYIIDKERGGPIAAVVLFTDGGNNTGIDVKV"
BASE COUNT         1031 a         1754 c         1729 g          883 t
ORIGIN      
        1 tgcgctgctg atgggcgtgc cgggccttgc gaagacgctg ctggtgagca agctctcgga
       61 aacgctcacg ctgaagttct cgcggattca gttcacgccg gatctaatgc cgatggacat
      121 caccggcacg gaaatcctcg aagagaaccg cagcacgggt aatcgcgagc ggcggttcct
      181 cgaaggtccg ctcttttcca acatcattct ggcggacgaa atcaaccgca cgccgccgaa
      241 gacgcaggcg gctctgctcg aagccatgca ggagcggcag gtgacggttg gccgcgtgcg
      301 ccatccgctg actgacccgt tcttcgtcct ggcgacgcag aacccgatcg agcaggaagg
      361 gacctatccg ctgccggaag cccagcaaga ccgctttatg ttcaaggttt tcgtgaacta
      421 tccgaatttc aacgaagagt tcgaggtcgc ccgccggacg accgccgtcc agaccgaaaa
      481 catccagccg gccctctccg gccaggagat catcgaactg cagcggacgg ttcgcgaagt
      541 gccggtcagc gaccacatca tccgctacgc cctctcgctg gtgcggcaga cgcgcgtcgg
      601 gcaaaacggc gtgcctgatt tcgtcaacga ccagatcggc tggggcgccg ggccccgcgc
      661 cgtgcagttc ttgatcctgg gcggcaaggc ccgggctctc ttgcgcggcc gcacgcacgt
      721 cagcaccgac gacatccagg ccctcgccaa gccggtcctc cgccaccgcc tggtcgtcaa
      781 cttcgccgcc cagagcgacg gcatcacagc cgacaagatc atcgaccgcc tgatcgccag
      841 cacccccacc aaggaagacg agctcaccag cgatgcccgg ttcaaaaaga tttttgcatc
      901 ctgaagcgat caagcggatc tcgcggctgg aagtccgggc gcggcacatc gtcgaagggt
      961 ttctttcggg gatgcaccgc agcccctatt tcgggcagtc ggtcgagttc ctgcagcacc
     1021 gcgagtacgc cctgggcgac gacctgcgcc acgtcgattg gaaggtctgg gcccgccagg
     1081 accggctgta cgtcaagcag ttcgaggaag agaccaacct ccgctcgcac ctcttggtcg
     1141 atgtctccaa cagcatggcc tacggcagcg ggccgctgaa caagtacgaa tatgcctgca
     1201 cgctggcctg ctcgctcgcc tacctgatcc tgcggcagca ggacggcgtc ggctgcatca
     1261 cgttcgacga gaaagtccgc gtgacggtgc cgatccgcac caagcacaac cacatcttca
     1321 gcatcgtcga ttcgctcgcc gtccaggagc cgaaagaaaa gaccgacatg tacgccatcc
     1381 tccgccaggt cgccgaaacg gcgccgcggc gcgggctgat ggtgctcgtc tcggacctct
     1441 tggcagatcg cccacggctc atcaaggggc tcaagctcct gcggcagcgc gggcacgacg
     1501 tgctcgtgtt ccacgtgatg gacgacgaag agctcgactt tcccttcagc gggccgatgc
     1561 gattcgaagg gctggaaacg cccgacttcc tgaattgcaa cccccgcgcc cttcgcgacg
     1621 gttacctggc cgccctgcaa gcctatctcg acgacatccg ccacgcctgt gcccggaaca
     1681 gttgcgacta cgccctcgta agaacgggag agccgctcga tgcggcgctg gcgacgttcc
     1741 ttagtaagcg aatcaccgta atgaatacct actagctgtt agtcctcgga cccacgctcc
     1801 gcgtgggacc gcacggttgg acgctccgcg tccgggctgc gacgcagagc gtcgaggctg
     1861 gcgttcccac gcagagcgtg ggaacgagca actgaaacaa gaagaccagt aacgctcgcc
     1921 tccaccactc accactcact gcccgctact tccgacccgt gcaattcgtc caccaaatcc
     1981 tgacctgggg cttcctgctg gcgctggtgc cgctgttgat ccacctgatc aacatgatgc
     2041 ggcatcgccg cgtcaaatgg gccgccatgg agtttttact ggcgagctac aagaagcacc
     2101 gcaagtgggt ctggctgaag cagctcttgc tcttgctctc acggatggcc gtcgttgcgc
     2161 tcatcgtggc gatgctggcg cagcttaaga cccgcgacca gtggctggcg atcttcggcg
     2221 gccgcgtaac gcaccactac gtgctggtcg atgacagcta ttcgatgtcc gaccgcgtcg
     2281 cgggcgcgac cgcgatggac gccgccaagc aggtgatggc cgccatcgtc ggccgggcca
     2341 cgcaggaaga cagcgctcag aagctgacgc tgatccggtt ttcgcaggcg cggggggcgg
     2401 aggagagggg acagggaaca ggggacaggg gacagggaac aggggacagg ggacaggggg
     2461 aagaggacag aggacagaag acagaggaca gtgtttctca atccgcaatc cgcaatccgc
     2521 aatccgcaat tcccgccgac gtcgccgact tcaacgccga gccgattgat tccaacttcg
     2581 aggtgacgct ggagcgaaag attcgcccgt ttgaggcgac gcagcttgcc ctggggcccc
     2641 tcgatgcgct gtcggtcgtc aagcagctcc tcagccagac caaggacgaa acgagcatcg
     2701 tctatctgct ctccgatttc cgaacgaagg attgggggag cccgaccgag tatcgcgagg
     2761 ccctgggtca gctccgccgc tcgcaggccg aggtgcacct ggtcaactgt gccaagagca
     2821 gcgagccaaa tctgggcgtg atctccatcg agccggctga cgagacgcgg gccgcaggcg
     2881 tgccgctgtt tgtgaacgtc aaggtgaaga acttcggcgc caaagccgcc agcaaggtgc
     2941 agcttaagct gcagtcggcc ttttatccgg cggatgacgt gaccgcatcg cagccggaat
     3001 cgctcaaggg ggaggtcgaa gacctcgcga cgctcctgat cgagtcgatc ggcccgggcg
     3061 aggtggtcga tcgccgcgtc caggtctatt tcccgcagcc gggcaagcac gtcgtcgagg
     3121 ccagcctgcc agaagacccg gtggaagccg acaaccaccg ctggtgcgtg atcgacttcc
     3181 ccgacggcga gcgggtgctc gtcatcgacg gcagcgtcga tcagcagcac gcctatttcc
     3241 tggaggtcgc cttccgcccg ctggagcggt cgaatacggg catccggccg gaggtgaagc
     3301 ccgcgtcatt cctgcgcgat gccacgctgg aaacgctcaa cggctattcg gccatctatc
     3361 tgctcgacgt cagccgcctg gatggccggg cggcggacac gctcgaggcg tatgtgacgg
     3421 gcggcggcgg cgtggcgatc ttcgccgggc cgacgatgaa caccggctac tacaaccagg
     3481 cgctctacaa ggaaggccag ggcctgctgc ccgcgccgct cggcctggaa acggacctcg
     3541 cgccggcgct ggaccccacc gagcccgacc tggagctgac cagccatccg gtcttctcgt
     3601 tcttcaccac ggaaacgaac ccgctcatcc gcggcgtgaa gatcgaccgt tatcgacagg
     3661 tcgccgacca gtggaagccc ggccccgaat cggctgtcga aattatcggc cgcacgcgcg
     3721 acaagagccc gctgatcatc gagaagcggc tgggccaggg ggaagtgctg ctgtttttga
     3781 ccacgctcgc ccccgattgg aacgactggg ccaaaaatcc gagcttcgtc gtcgtgctgc
     3841 tgaagacgca atcgtacctg gccacggcca agcggctcga tgacccgcga ctggtcggca
     3901 cgcccgtcga tgtggctctc gaagcggcca agtaccggcc cgacatgacg ttcatcgtgc
     3961 cgggcgaaaa gaccggctcg cggcagaaga tcgaccgccg agccgagcct ccctccggca
     4021 gccaggccga tgcggccacg ctggtcgccg cactcggccg cacgccggtc gcggggaaat
     4081 cgcgcggcga gaccgaccgc gccggcgtct atgaagcctg gccggtcacg acgcggggcg
     4141 agatcgacct caagcggtgg gccctgaacg tcgagccgga cgaaggggac ctgacgccga
     4201 tcacctcgac cgacctgatc gcgaagctgg agcctgtgaa ggtgaattac cacctcgccg
     4261 accagtatca gcaggaggat gtcgccagca cgggctacaa cctcagcacg ctgttactga
     4321 tgggcctgat cgccctgctc atcggcgagc aggtgctcgc ctattccgcc agttaccacg
     4381 ttacgcccgg agccgtccgc tgatcccgct cttgccggtg ctggcggagg catccaccaa
     4441 catccaatac aagctggccc gtctgcaaac gctcaccgag tggtggcact ggatcgcgct
     4501 ggtggcggtg agcgtggcga tcgtggcgtt cgtcgtctgg atgtacatcc gcgacagtgt
     4561 tgaattgccg cgcggcctgg ccatcacgct ctgcctgctg cggctggtgg ctttcttcgg
     4621 gatcctgttc tacttctttg gcctggaaaa gcgggccgag cgaaagctgg tgaagaattc
     4681 gcgggcgatt ttactgatcg acaccagcca gagcatgggg ctgcgcgaca gcgactcctc
     4741 gaacgttccc gccgccatga gccgcgtgga gcacgtcgcg gcggagttgg ccagcggcag
     4801 catgctcgaa aagctgcgcg agaagcacga cgtgatcgcg tatcggttcg accaggggga
     4861 gaacccggtc gaaatcgcct cgtatccccg caagccgccg ccggaagaaa cggccgaaac
     4921 gacaatgtcg gagcaaagcc ggctcaacca gatcgtggat gaggcccggc tgctggcgta
     4981 tgtggcagcg ggcgttctgg cggtctcggt gatcgcgggg ctggtgtaca tggcgtttgg
     5041 ctcgcgggcg gtcgccggcg agcccacttc gtgggccctg ctcgtgagca tgacgacgct
     5101 gatcgcggcg gtggtgatcc tggcggtcgc cagcctgcgc agctcggagg ttggccccct
     5161 ggccgtcctc ggactgcgcg aggtcaagcc gttggaagag gagcaagacg agacgagcac
     5221 cgaagagccg gcggagattc cgacggtcga ttgggcggcg cagcttgctc cgcgcggcgg
     5281 ggagacgcgg atcggcgaca atctcaagta catcatcgac aaggagcgcg gcggcccgat
     5341 cgccgcagtc gtcctcttca ccgacggcgg caacaacacg ggaatcgacg tgaaggt
//
LOCUS       DASUGB010000793         4447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1475859, whole genome shotgun sequence.
ACCESSION   DASUGB010000793 DASUGB010000000
VERSION     DASUGB010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475859"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..600
                     /locus_tag="VFV87_15160"
     CDS             151..600
                     /locus_tag="VFV87_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5105157.1"
                     /translation="MDVLVDTGVLLRAFDRSLPEQPTIFRAFRELWSQNHQLATTLQN
                     IAEFWNVSTRPVQARGGFGLPVAIVDARVKLIERLGDVLPFPDRAYQEWRRLVAAHQI
                     VGVAVHEAKLVATMNVAKIQHVLTMNAADFQRYPGLTIWTPDDVVQS"
     gene            606..2285
                     /locus_tag="VFV87_15165"
     CDS             606..2285
                     /locus_tag="VFV87_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105158.1"
                     /translation="MVLYAFVTAVAAAYCLLAIWVAVGSGLWFRRMCALLGALALLVP
                     IRAYEPLILFGMSSAMLIAGWGAVRLWEDRKQSSCGGRPNPLKFHLRDILLSCAVAAA
                     ASWMISVLADKGVTVHWRLAMGSAALLAGVSSLAVGVVRYRGWRWRLPLLIAGIIAAA
                     AIHVVALDCWWDRAEMLGLLTASWRRSDIPLAILGFSIQVGQFALWSLFIAAMAIAVT
                     GRWNRSIYRTIVRGTCAMLLTAAIVFTGWVYWRMLDVPRWSPSAANRPNALPRIIELA
                     TAAKRATPAQMDEIHAELFSLLDRPSFVVWPDDRPTTQELGASNELSLTRNLTRGLQA
                     RSLAVQAQGRYDEAADQCLAVLRLAPMLDAEGIGVHRWEAEAVRHMGHAWLAAFREKL
                     SPDKARESARVILTVAAGREPIEATELRDRIWNDAITWRDLLKRTLLTELQGYPLPPL
                     MLREAEQFSVQESCIARLLALDLLIRAYRQEHGQFPNSLAEVALPDFPDLATDPYSAE
                     PFVYQPSEQGFILYSVGQNGIDEGGKLTNMPTYMGQSHKAFDLDVDTMTRP"
     gene            complement(2364..2696)
                     /locus_tag="VFV87_15170"
     CDS             complement(2364..2696)
                     /locus_tag="VFV87_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5105159.1"
                     /translation="MSKTRQRALEYVEPIGARVLVRKDEPKRQTKGGIALPDQAEIPT
                     ITGRIVAISAQIEKDEDVPLRQYDKILFHPKNAIPVDFESDNQLFVVPVEDVVAVFRR
                     EPREGNET"
     gene            2957..3601
                     /locus_tag="VFV87_15175"
     CDS             2957..3601
                     /locus_tag="VFV87_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5105160.1"
                     /translation="MPTFLPVQRREKIGSANTRRLRKTGVIPAVLYGHGEATLSLSVP
                     ADALLGVIRHGQKLVTLQGDAADAAFIKAVQWDVWGKDVLHVDLLRVSETELVETTVR
                     IELRGTAPGISEGGIIEHLVHEIEIDCPAASVPDKITVMINELHLDGSIAASDVKLPE
                     GAKLLSDPDAIIVHCIKPHVAEETPAAGVAEPGAVEPELIRKEKAEEGEEAAAE"
     gene            3624..4187
                     /gene="pth"
                     /locus_tag="VFV87_15180"
     CDS             3624..4187
                     /gene="pth"
                     /locus_tag="VFV87_15180"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5105161.1"
                     /translation="MKLIVGLGNPGGQYKDTRHNVGFEVARLLAQKFGSAAPRGRFQG
                     ETVEARIAGRKALLLTPLTYMNLSGASVLAARDFYKIEHPDILVVCDDFNLPLGKLRL
                     RVKGTAGGQNGLADILQRLGTDEIPRLRIGIGSPPPGRDPAGYVLSRFAKDEQPVIAE
                     SIQRAAEAAAAWVESGIAEAMNKYNAT"
BASE COUNT          800 a         1360 c         1434 g          853 t
ORIGIN      
        1 aatcattccc gcaggcgacc cttctgctcg accaaattgg gatgaattgg aacgacaatt
       61 catcgccctc agcacaccgg gaccgtcgct cccgtccgac ttctgccggg ccgacatcta
      121 tctcaaccat gattagcgcg ggttgaccga atggatgttc tggtcgatac cggcgttctt
      181 ctacgggcgt ttgaccgatc gctgccggag cagccgacga tctttcgcgc cttccgcgaa
      241 ctctggtcac aaaaccacca actggcgacg accttgcaga acatcgccga gttctggaat
      301 gtctcgacac gtcccgtgca ggcccgtggg ggattcggct tgccggtcgc gattgtggat
      361 gctcgcgtca agctgatcga gcggctcggt gacgtgctgc cgttcccaga ccgtgcctat
      421 caggagtggc ggcgattggt cgccgcccat caaatcgtcg gcgtggcggt tcacgaggcc
      481 aagctcgttg cgacgatgaa cgttgcgaaa atccagcatg tgctcacgat gaacgcggca
      541 gactttcagc ggtatccggg cctgacgatt tggacgccgg acgatgtggt gcagagttaa
      601 cgattatggt cctctacgcc ttcgtcactg ccgtagctgc cgcctactgc ttgctggcca
      661 tctgggtggc ggtcggctcc ggcctctggt ttcggcggat gtgcgcgttg ctgggcgcgc
      721 tggcactgct cgttccgatc cgcgcttatg agccgctgat tctgtttggt atgtcctcgg
      781 cgatgctgat cgctggctgg ggcgcggtca ggttgtggga agaccggaaa cagtcgagtt
      841 gcggcggtcg gccaaatcca ctgaagtttc atttgcgcga catcctcctt tcgtgtgcag
      901 tagccgcggc cgcatcgtgg atgatcagtg tcctggctga caagggcgtg actgttcact
      961 ggcgactggc gatgggctcc gcggcgctct tggcgggcgt ttcctccctt gctgtaggcg
     1021 tcgtccgcta ccgcggctgg cggtggcggc tgccgctctt gatcgccggc atcatcgccg
     1081 ccgcagccat tcacgttgtg gcgctcgatt gctggtggga tcgtgcggag atgctagggc
     1141 tgctaaccgc gagctggcga cgctccgaca tcccgctcgc catcttggga ttctcgatcc
     1201 aggtggggca gttcgctctc tggtcgctgt tcatcgcggc catggcgatc gccgtaacag
     1261 gccgctggaa tcgttcgatc tacaggacga tcgtccgcgg gacgtgcgct atgcttctta
     1321 ctgcagcgat cgtttttaca ggctgggtct attggcgaat gctggacgtg ccaaggtggt
     1381 cgccgtccgc cgcaaatcgc cccaacgcgc tgccgcgaat catcgaattg gcgacggccg
     1441 ccaagcgtgc cactcccgct caaatggacg agattcacgc cgagctgttc tcattgctgg
     1501 acaggccaag ctttgtggtc tggccggacg atcgcccaac gacgcaggag ttgggcgcaa
     1561 gtaatgagtt gtcattgact cgcaatctga cacgcgggct gcaggcgcgc agcctcgccg
     1621 tgcaggccca aggccggtat gacgaagcag ccgaccagtg cctggcggtc ttgcgcctgg
     1681 cgccgatgct cgatgcggag gggatcggcg ttcaccgctg ggaagcagag gcggtgcggc
     1741 acatggggca tgcctggctg gcggcctttc gtgagaagct ctcgccggac aaggcgcgcg
     1801 aatcggcccg agtcatactg acggtcgcgg cgggccgcga gccgatcgag gcgacggaac
     1861 tgcgcgaccg catctggaac gatgcgatta cctggcggga cttgctcaag cgcacgctgc
     1921 tgaccgagct tcaaggttat ccgttgcccc cgctgatgct ccgcgaagcc gagcagtttt
     1981 ctgtgcaaga atcgtgcatc gcccggctcc tcgcgctgga tctgttgatc cgcgcctacc
     2041 gccaggagca cggccaattc ccgaactcgc tcgcagaagt tgctctccct gactttcccg
     2101 acttggcgac cgacccctat tccgctgagc cgttcgttta tcagccctcg gagcaaggat
     2161 tcatcctgta tagcgtgggc cagaacggca tcgacgaagg cgggaagctc accaacatgc
     2221 cgacttacat gggccaatcg cacaaggcgt tcgacctcga cgtcgatacc atgacccggc
     2281 cctgaaacac actctggggc ggggcactgc tatttgcagc caggccacca atcgtggccc
     2341 agcgacccgg ttgagtccgc cagttacgtc tcgttcccct cccgcggctc gcggcggaag
     2401 acggcgacga cgtcttcgac ggggacgacg aagagctggt tgtcggactc gaaatcgacc
     2461 ggaatcgcgt tcttggggtg gaagaggatc ttgtcgtact gccgcagggg gacgtcctcg
     2521 tccttctcga tctgggcgga aatggcgacg atgcggccgg tgatggtcgg aatttcggcc
     2581 tggtcgggca gggcgatgcc ccccttcgtc tgccgcttcg gctcatcctt gcggacgagg
     2641 acgcgggcgc cgatcggctc gacgtattcc agggcccgct gacgggtttt ggacatgaat
     2701 ggctcggagg aggcgcctaa ttggcagcta gtttgtcgac tttggcggca aaagccggca
     2761 cgtcacaacc ttactcagga caaccagttg cggcaaggga aagtcgcctg gggcagacat
     2821 tcccgtctgc cggcaggcaa gaatgcccac cccacgggcg gccttgtcag cgggcgtgga
     2881 tttgccacaa tgttgggttt cccctcaaga gacttaacgc ccattcgccg cgccgccggc
     2941 gaagagcgaa gatcctatgc ccaccttcct acccgttcag cgccgcgaga aaatcggcag
     3001 cgccaacacg cgccgccttc ggaaaactgg cgtgattcct gccgttctgt acggacacgg
     3061 cgaagcgacg ctcagccttt cagtccctgc tgatgccttg ctgggcgtca tccggcacgg
     3121 ccaaaagctc gtgaccctgc agggcgacgc cgccgacgcc gcctttatta aagcggtcca
     3181 gtgggatgtg tggggcaagg atgtgctgca cgtcgatctg ctccgtgttt ccgagacgga
     3241 gctggtcgaa acgaccgtga ggattgagct ccgcgggacc gccccgggga tcagcgaagg
     3301 gggcatcatc gagcacctcg tccacgagat tgagatcgat tgcccggctg cttccgtgcc
     3361 ggacaaaatc acggtcatga tcaacgagct gcacctggac ggatcgattg cggccagcga
     3421 cgtaaagctg cccgaagggg cgaaactgct gagcgatccg gacgcgatta tcgtccactg
     3481 catcaagccg cacgttgccg aagaaacgcc tgccgccggc gtggccgagc ccggcgcggt
     3541 cgaacccgaa ctgatccgca aggagaaggc ggaggaaggc gaggaggctg cggccgaata
     3601 gccttttcgc tggtgcggtg gccatgaaac tgatcgtggg gctgggaaat cccggaggac
     3661 aatacaaaga cacccgtcac aacgtgggat tcgaagtggc ccgcctgctg gcgcaaaagt
     3721 tcggcagcgc cgcgccgcgg gggcgattcc agggcgagac ggtcgaggcg cgcatcgccg
     3781 ggcggaaggc cctgctgctg acgcccctga cgtacatgaa tttgagcggc gccagcgtcc
     3841 tggccgcccg cgatttttat aagatcgaac atccagacat cctggtcgtc tgcgacgact
     3901 tcaacctgcc gctgggcaag ctccggctgc gggtcaaagg cacggcgggc ggccagaacg
     3961 gtttggcaga tatcctgcag cggctgggga ccgatgagat cccgcggctg cggatcggga
     4021 ttggcagtcc gccgcccggc cgcgatcctg cggggtacgt gctcagccgc ttcgcaaaag
     4081 acgaacagcc ggtgattgcc gaatcgatcc agcgggccgc agaagcggcc gccgcgtggg
     4141 tggaatcggg aatcgccgag gcgatgaata agtacaacgc aacgtaattc gacatgcaac
     4201 aaagccgcga ccagtggtcg cggttcccag cacaattccc gcgaccgatg gtcgcggctt
     4261 tgagtcccgt gaccaccggt cgcggctttg agacacgaag gagtttccct tggcacagaa
     4321 cgtctatgaa tgcatggtga tcttggaccc gaacaagtat gcccaggatc ccggcgggct
     4381 cggctcgcaa atccccaacc tcgtggcgaa gttcggcggc gaggtgctcg tcagccgctt
     4441 gtggaac
//
LOCUS       DASUGB010000794         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1337351, whole genome shotgun sequence.
ACCESSION   DASUGB010000794 DASUGB010000000
VERSION     DASUGB010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337351"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFV87_15185"
     CDS             complement(<1..512)
                     /locus_tag="VFV87_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105162.1"
                     /translation="MRCFIAGGLLGISATLVLADTPAERAASLSKLSPDQKEELLRKK
                     QRFDALDPAEQQRLRELHAALSAGADAEQLHTTAVRYANWLKGLQPGERAEVLSLPAD
                     KRIVRIREIVSQQERQRFRDLSNLPPNDQEFVYQWLDKFVADNEKAILEAIPSYRDRR
                     DIRETKDDRAR"
     gene            complement(553..1059)
                     /locus_tag="VFV87_15190"
     CDS             complement(553..1059)
                     /locus_tag="VFV87_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5105163.1"
                     /translation="MIEENTLSTEEAIDQEIVAYLDGELDPAAAARVERQMAENPRYS
                     ARLNQLQKAWDLLDTLGPSEADDNFTHSTVAMVALKAQDEVQGKDEAASRRQRLTWAA
                     LAGLAMAAAIGAYVVVDRQLNSENRELVRDLPVIERVDEYRNVESVEFLQELQKEGLF
                     AAEVDHGQ"
     gene            complement(1125..1754)
                     /locus_tag="VFV87_15195"
     CDS             complement(1125..1754)
                     /locus_tag="VFV87_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105164.1"
                     /translation="MLQVRDGNAAAFEELVARYQNRLLTVLEHLIGNREQAEDLAQEV
                     FLRVFRARERYEPEAKFSTWLFTIANNVASNARRSHSRRREVGVPERSNGSDSSPMQL
                     DQLAKAASGLMPARALDKAEQSEMVREAVKALSERQRMALLLSKFEGMSYQDIAHTMG
                     LSVQAIKSLLSRARVNLKEILTPYIEQGIRPEDAPRGANGSPEHDDEIT"
     gene            complement(2012..>2807)
                     /locus_tag="VFV87_15200"
     CDS             complement(2012..>2807)
                     /locus_tag="VFV87_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105165.1"
                     /translation="AITALNRKSKIRNPKSEICKLRSAIPFALILAAILATWWLCSHR
                     IDRFLLPAWPFAALLAAIGAASLDGLWPRRVVLAIAGLGLAWCFLADCSTLVGDNRWF
                     VALEQLRRDHPWPDGTRLRVKLEHQWLNENIQPGRAVLLVGDAAGFDLEMPVYYNTCF
                     DNCLLCDWMLDQSPRQKSPGQWRDELASRRIAWVLVDWPEIARYQSPGNYGFDPRFSP
                     ELIEELVRHRVIGPPETIRSQTEGRPPERYPVRGVAAKQPAGPGVP"
BASE COUNT          474 a          902 c          883 g          548 t
ORIGIN      
        1 cgggctcggt cgtccttcgt ctcccggata tcgcgccgat cgcggtagct gggaatagcc
       61 tccagaattg ccttctcgtt gtccgcgacg aacttgtcga gccactgata gacgaattct
      121 tgatcgttgg gcggcaggtt agataagtcc cggaatcgct gcctttcctg ttggctgacg
      181 atttcccgga tgcggacgat tcgtttgtcc gccggcaggc tgaggacttc ggcccgttcg
      241 cccggctgga ggcccttgag ccagttcgcg tatcggacgg cggtggtgtg gagctgctcg
      301 gcatcggccc cggccgagag cgcggcgtgc agctcgcgca gccgctgttg ctcggcaggg
      361 tccaatgcgt cgaaccgctg cttcttgcgg agcagctcct ccttctggtc gggcgatagc
      421 ttcgatagcg aggccgcccg ctccgccggc gtgtcagcga ggaccagcgt tgcggagatg
      481 cccaacaggc ccccggcaat gaaacagcgc atcgtcaaac atgcgaaccg caatccgcaa
      541 tcggcaattg ccttactgtc catgatccac ctccgcggca aacagcccct ctttctgcag
      601 ctcctggagg aattcaacgc tttcgacatt gcggtattca tcgacccgct cgatcaccgg
      661 cagatcgcgg accagctcgc ggttctcgct attgagctgc cgatcgacca cgacgtacgc
      721 cccgattgcc gccgccatcg ccagaccggc cagggcggcc caggtaaggc gctgcctgcg
      781 gctggcggcc tcgtccttgc cctgaacttc gtcctgggcc ttgagggcga ccatcgccac
      841 ggtgctgtgg gtgaaattgt cgtcggcctc gctggggccg agcgtatcga gcaggtccca
      901 ggccttttgc agctggttca gccgggcgct atagcgcggg ttctcggcca tctgccgctc
      961 gacgcgggcg gcagccgccg ggtcgagctc gccgtcgaga taggccacga tctcctggtc
     1021 gatcgcttct tcggtagaaa gggtgttttc ttcgatcatg gcgggctcgt ttggtctggc
     1081 atttggccgg caggctgggg aacatgcgcc gcttcgcgcc gcggctaagt gatttcgtcg
     1141 tcgtgctccg ggctgccgtt cgcgccgcgc ggggcgtctt ccggccggat tccttgctcg
     1201 atataaggcg tcaatatctc cttgagattg acgcgggccc gcgagagcag cgacttgatc
     1261 gcctgcaccg acaaacccat cgtgtgggca atgtcctggt agctcattcc ttcgaacttc
     1321 gacagcagca aagccatccg ctgccgctcg ctcaaagcct tgaccgcctc gcggaccatc
     1381 tccgactgtt cggccttgtc cagcgcgcgg gccggcatca gaccgctggc cgccttggcc
     1441 aactggtcga gctgcatcgg cgaactgtcg ctgccgttgc tccgctcggg cacccccacc
     1501 tcgcggcggc gggagtggct ccgccgggcg ttgctggcca cgttgttagc gatcgtgaat
     1561 agccaggtgc tgaacttcgc ttccggttca tagcgctccc gcgctcgaaa cacccgcagg
     1621 aaaacctcct gcgccaagtc ctcggcctgt tcgcgattgc cgatcagatg ctcgagcacg
     1681 gtgagcagcc ggttctgata gcgcgcaaca agctcctcga acgcggcagc attgccgtcg
     1741 cgcacctgca gcatgagccg cacgtcggga tcgtgcagat cgtatcggta aggctcggtc
     1801 ggcagtgcca agtcagtcag cgccaaggca ggatcgctct ggctcgagat cggatggaac
     1861 cggggattcg tgcttcgctc gtatctcctc cgttcgcctg tgctccgttc acgcagccgt
     1921 cctaagtcta tggcaattca ctacatgggg ccagggtttg caccgccgca gcggttgtgc
     1981 cgccgcgtgg gacagtacct gacaaccacc cctatggaac cccaggcccc gccggctgtt
     2041 tcgcggcgac tccccgcacg gggtatcgct cgggcgggcg gccttccgtt tggctgcgaa
     2101 tcgtttctgg gggaccgatc acacggtgcc ggaccaattc ctcgatcaat tccggcgaaa
     2161 atcgcgggtc aaagccgtaa ttgcccggcg actggtagcg ggcgatctcc ggccaatcga
     2221 ccagcaccca ggcgatgcgt cggctggcca gttcgtcgcg ccactggccg ggcgatttct
     2281 ggcgcggcga ctggtcgagc atccaatcgc agagcaggca attgtcgaag caggtgttgt
     2341 aatagacggg catttcgagg tcgaacccag cggcgtcgcc caccagcagc acggcccgcc
     2401 ccggctgaat gttttcattg agccactgat gttcgagctt gacccgcagc cgtgtgccat
     2461 ccggccaggg atgatcgcgg cggagttgtt cgagcgcgac gaaccagcga ttatcgccga
     2521 cgagcgtcga gcaatcggcc aaaaaacacc aggccagccc cagccctgcg atggccaaca
     2581 ctacgcgacg cggccaaagt ccatcgagag atgcggcccc gatcgccgcc agcaaggcgg
     2641 caaacggcca agccggcaaa agaaaacggt cgatgcgatg ggagcagagc caccaggtgg
     2701 cgaggatcgc ggccaggatg agggcaaaag ggattgcaga tcgcagcttg cagatttcgg
     2761 attttggatt tcggattttg gatttccgat tcaaggcggt gattgcc
//
LOCUS       DASUGB010000795         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_365599, whole genome shotgun sequence.
ACCESSION   DASUGB010000795 DASUGB010000000
VERSION     DASUGB010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365599"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /locus_tag="VFV87_15205"
     CDS             <1..2623
                     /locus_tag="VFV87_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5105166.1"
                     /translation="LRFLVDVGLDYLSLDRKGPTLSGGEAQRIRLASQIGSGLTGVLY
                     VLDEPTIGLHPRDNTKLLKALQKLRDQGNTLVVVEHDREVIASADHVLDFGPGAGRLG
                     GQIVADATPDKLGKLSGSVTGPYLSGKKAIAVPANRRLEGSGLGVRGSGKQKKGKLLA
                     TGNIDKTPTSPEPRAPNPCLEILGARPNNLKNINVAIPLGTLTAITGPSGSGKSTLVD
                     EILYAAIARKLHRASLVPGKHDDIKGLEHINKVIRVDQQPLGNSPTSNPATYTGCFEL
                     IRTLFAQLPDAKLRGYSGRRFSFNVPGGRCEACEGNGQRRIEMHFLPDVWVECETCRG
                     KRYNPETLAVTFHGRSISDVLDMTCGDAVKLFENIPKIRRILQTLCDVGLDYLTLGQP
                     APTLSGGEAQRVKLAAELSRPDTGRTLYLLDEPTTGLHFDDLAKLLEVLQRLVDLGNT
                     VVVIEHNLDVIKCCDWVIDLGPEAGEAGGQVVACGTPEQILDFGLPILDSRTAKKSPQ
                     SKIQNSKSKIVSHTALALAPVLAAGPHAERKKYDPAAELEKRDGDLDITEVGKTAKMP
                     WEMDGRAWHTRDRVGRTGEPCRWDGEILARVVDRIHELGEFAETDWSERSVVEITAPK
                     KSDGWFFHAITGEVWLVKLKFRVYRGTFKRDELQQRIKLKTLNELAELPIYGNEPRVK
                     VKNLQGPWQEVEIRAHSLAEIDTPEFWQFLEEAVAGFAKFTEKAAVNPEDISPWKVLG
                     QKWHFLRKGFPPGKTVAWPVEVLEELCELLQQVAPGGQFLWNNQQLVHYMVPHQREPW
                     ATIQTKKTHALLLHLAGPKGKFGLGRLLDLGADRDLDDTRPDRDIVRLQFVEEDHLHR
                     GELDKFLQEHLASVNGK"
     gene            2670..3320
                     /locus_tag="VFV87_15210"
     CDS             2670..3320
                     /locus_tag="VFV87_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105167.1"
                     /translation="MEQLIPPTMLFRFAAPCLFSAEKWTAKGCALGAEHRLPCFGELE
                     GRPSFADVRLAWNERGLLVNVLVQGKQQPPWCRDSRIEDSDGVQLFIDTRNTQNIHRA
                     GRFCHRFALLPIGAGGRLDEPVAALLAINRAKESPREIEHGALQIRATIRPDGYMLEA
                     HLPAATLTGYSPADQPALGFSYAVTDRELGWQTSAVGRELPFAEDPSLWGTLELVR"
     gene            complement(3346..>3594)
                     /locus_tag="VFV87_15215"
     CDS             complement(3346..>3594)
                     /locus_tag="VFV87_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105168.1"
                     /translation="DDGEDDDWDDDDDDELDEEEDEELDEEDDDLDDEWEEVGDDEEL
                     DDELDDDEDDDEEDDWDDDDEDDWDDDDEDAGEGEDPA"
BASE COUNT          675 a         1171 c         1148 g          600 t
ORIGIN      
        1 actccggttc ctcgtggacg tcggactcga ctacctgagt ctcgaccgca agggcccgac
       61 cctctccggc ggtgaggccc agcgcatccg cctcgcctcg cagatcggca gcgggctcac
      121 cggcgtcctc tacgtcctcg acgagccgac gatcggcctg cacccgcgcg acaacacgaa
      181 attgctcaag gcgctgcaga agctccgcga ccaggggaac acgctggtcg tcgtcgagca
      241 cgaccgtgaa gtgatcgcca gcgccgacca cgtgctcgac ttcggccccg gcgccggccg
      301 cctcggcggc cagatcgtcg ccgacgcgac gccggacaag ctcggcaagc tcagcggctc
      361 ggtcaccggg ccgtatttga gcgggaagaa ggcgattgcc gtgccggcga accggaggct
      421 cgagggttcg gggctcgggg ttcggggttc gggaaagcag aagaaaggca agttgttggc
      481 aacaggaaac atcgacaaga ctcccaccag ccccgaaccc cgagccccga acccctgcct
      541 cgagatcctc ggcgcccgcc ccaacaacct caagaacatc aacgtcgcta ttccgctggg
      601 cacactgacg gcgatcaccg gtcccagcgg cagcgggaag agcacgctgg tcgatgagat
      661 cctatacgcg gccatcgccc gcaagctcca ccgggcgagc ctcgtgccgg gcaagcacga
      721 tgacatcaag gggctggagc acatcaacaa ggtcatccgt gtcgatcagc agccgctggg
      781 gaattcgccc accagcaacc cggcgacgta caccggctgc ttcgagttga tccggacgct
      841 ctttgcccag cttcccgacg cgaagcttcg cggctattcg ggccggcggt tcagcttcaa
      901 tgtgccgggc ggccggtgcg aggcctgcga ggggaacggc cagcggcgga tcgagatgca
      961 ctttctgccc gacgtgtggg tcgagtgcga gacctgccgc ggcaagcgct acaaccccga
     1021 gacgctggcg gtcacgttcc acggccgctc gatcagcgac gtgcttgaca tgacctgcgg
     1081 ggacgcggtc aagctgttcg agaatattcc caagatccgc cgcatcttgc agacactgtg
     1141 cgacgtgggg ctcgattatc tcacgctcgg ccagcccgcg ccgacgctct ccggcggcga
     1201 agcccagcgg gtcaagctcg ccgccgagct gtcgcgtccc gacactggcc gcacgctcta
     1261 cctgctcgat gagcccacga ccggcctgca cttcgacgac ctggccaagc tcttagaagt
     1321 gctccagcgg ctggtcgatc tgggcaacac ggtcgtcgtg atcgagcaca acctcgacgt
     1381 catcaagtgc tgcgactggg taatcgacct ggggccggaa gcgggcgaag cgggggggca
     1441 ggtggtggcg tgcggaacgc cggaacagat tttggatttt ggattgccga ttttggattc
     1501 aagaacagcc aaaaagtctc ctcaatccaa aatccaaaat tcaaaatcca aaattgtgtc
     1561 ccacaccgct ctcgccctcg cccccgttct cgccgccggg ccccatgccg agcggaaaaa
     1621 gtacgatccg gcggcggagc tggagaaacg tgacggcgac ttggacatca ccgaggtcgg
     1681 aaagacggcc aagatgccgt gggaaatgga cggccgggca tggcacacgc gggaccgcgt
     1741 cggccgcacc ggcgagccct gccggtggga cggtgaaatc ctggcccgcg tcgtcgatcg
     1801 aattcacgag ctgggcgaat tcgccgagac cgattggagc gagcggagcg tcgtcgagat
     1861 cacggcgccg aaaaaatccg acggctggtt tttccacgcg atcacgggcg aagtctggct
     1921 ggtgaagctc aagttccgcg tctatcgcgg cacgttcaag cgcgacgagc tgcagcagcg
     1981 gatcaagctg aagacgctca acgagctggc cgagctgccg atctatggca acgagccgcg
     2041 agtgaaggtg aagaacctgc aggggccgtg gcaggaggtg gaaatccggg cccactcgct
     2101 ggccgagatc gatacgccgg agttctggca atttctggaa gaggcggtcg ccggcttcgc
     2161 gaagttcacg gaaaaagcag ccgtcaaccc ggaggacatc tcgccgtgga aggtgctcgg
     2221 tcagaagtgg cacttcctgc ggaaggggtt tccgccgggc aagacggtcg cctggccggt
     2281 ggaagtgctc gaagagctgt gcgagctgct ccagcaggtt gcgcccggcg ggcagttcct
     2341 gtggaacaac cagcagctcg tccactatat ggtcccgcac cagcgcgagc cgtgggcgac
     2401 catccagacc aaaaaaacgc acgcgttgct cttgcacctg gccgggccga agggcaagtt
     2461 cggccttgga cggctgctgg atctgggggc cgaccgagat ctggacgaca cgcgtcccga
     2521 ccgcgacatc gtgcggctgc agttcgtgga ggaggaccac ctgcaccgcg gtgaattgga
     2581 caaattcctg caggagcacc tggcatccgt caacggaaaa tgaccaggct tcaagaacca
     2641 aatccgcaat ccgcaatccg aaatccgcaa tggaacaact cataccgcca acgatgctct
     2701 tccgcttcgc cgcgccatgc ctcttttcgg cggaaaagtg gaccgcgaag ggctgcgcgc
     2761 tgggcgcgga gcacaggctg ccgtgctttg gcgagctgga agggcggccg agctttgccg
     2821 acgtgcggct ggcgtggaac gaacgggggc tgctggtcaa cgtgctggtg caaggcaaac
     2881 aacaaccgcc gtggtgccgc gattcgcgga ttgaagacag cgacggcgtg cagctcttta
     2941 tcgacacgcg aaacacgcag aatatccacc gggccggccg tttttgccac cgcttcgcgc
     3001 tgttgccgat cggcgccggg ggtcggctgg atgagccggt ggcggccctg ctggccatta
     3061 accgggccaa ggagagcccg cgcgagatcg agcacggcgc gctgcagatc cgcgccacca
     3121 tccgccccga cggctacatg ctggaagcac atttgccggc ggcgacgctc accggctaca
     3181 gtcccgccga tcagccggcg ctcgggttca gctacgcggt gaccgaccgc gagctcggct
     3241 ggcaaacctc tgccgtgggc cgggagctgc cattcgcgga agatccgagc ctgtggggga
     3301 ctttggagtt ggtccggtaa tcactgcaat cggccggcgg cgcggtcacg ccgggtcttc
     3361 gccttcgccg gcgtcttcat cgtcgtcgtc ccagtcgtct tcatcgtcgt cgtcccagtc
     3421 atcctcttcg tcgtcgtctt catcgtcgtc cagctcgtcg tccagttctt catcgtcacc
     3481 gacttcttcc cactcgtcgt ccaagtcgtc atcctcttcg tccagttctt cgtcctcctc
     3541 ttcgtcgagc tcgtcgtcgt cgtcatcgtc ccagtcgtcg tcctcgccgt cgtc
//
LOCUS       DASUGB010000796         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_782681, whole genome shotgun sequence.
ACCESSION   DASUGB010000796 DASUGB010000000
VERSION     DASUGB010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782681"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFV87_15220"
     CDS             <1..386
                     /locus_tag="VFV87_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105169.1"
                     /translation="LEPYNFANYLDLAKASAQLGEPEAAEATLKLAITMQPQAADGYT
                     ALAQFYLESSRAKKARWYSQEAVRRQPTAEGYRLLASTCRLLGDTASAEAALALAGQL
                     ESERRSPPQTNPNRAAPPAAAGKQP"
     gene            425..2101
                     /locus_tag="VFV87_15225"
     CDS             425..2101
                     /locus_tag="VFV87_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX5105170.1"
                     /translation="MLAVALLPALLSVLLSVLLSAPAQAQSPASCPIRLTDATAASGI
                     KFRHTDGGSGQGYIIEGMASGIATFDYDGDGLIDIYFLSGAALKGTVLDSPARDALYR
                     NNGDWTFTDVTEQAGLGDLHHSLGVAAADYDGDGDQDLYVSNFGPNVLYRNNGDRTFT
                     DVTEQAGVGNGNKVGAGCSFFDMDGDGDLDLYVGNYVDFTYENHVPIVVKGRHFQAGP
                     QYYQPVADTLYRNNGDGTFTDVSGPSGIAAVAGPSMATLAFDYDDDGDADMFVCNDGK
                     PNFLFENDGHGKFQEKALVAGLAYNFYGKAISSMGVDCGDVDNDGRLDLFVTSYQSEM
                     PVLFRNLGGGLFEDGTSTARIATDFFAHVEWGTGQVDFDNDGDRDLFLACGHFDRIEL
                     LDDRTSLKVPNYVLMNDGQGRFTDVSKQCGSGLAVVESSRGVAFDDLDNDGDVDAVVL
                     NSNAAGTLLRNESPAGNHWLELRLQGGQRNLDGIGARVKVVSGERAQIAEVHSGRGYQ
                     SHYGTRLHFGLGQQQRVDRIEVRWPGGSVEHFPGGEPDRLLVLKQGSGRQ"
     gene            2270..3382
                     /locus_tag="VFV87_15230"
     CDS             2270..3382
                     /locus_tag="VFV87_15230"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5105171.1"
                     /translation="MWRRWFRSGFTLVELLVVIAIIGVLVALLLPAVQAAREAARRSS
                     CSNNLKQHALSMHNYHDTYKVFPALGIRPENNQGNFGGTNNGNWYGWLIATMPYIEQK
                     PLYDNIMTRVADPTVSLPTAWYNLNNNSDPWANQNWKFDLGVNMCPSSPKPTIRTESP
                     ALLSYKVCVGDDYHQNHFLPIDGRDNRGIFQMDRWIGMEAITDGTSQTVMMGECVMGG
                     QPTDVLGGVAVNVQQWSPQDCWNRVDPNNRKQLLASAGVRADFRPTGGRALDGRPYYV
                     GFATLVAPNGPTCHWGGVDGNEHEGTLSSFHPGGGQVAMADGHVQFISQTINAGNPTI
                     PDDGAGWPSNNRSGPSPYGVWGALGSKNGTEAIANQ"
     gene            3519..3989
                     /locus_tag="VFV87_15235"
     CDS             3519..3989
                     /locus_tag="VFV87_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5105172.1"
                     /translation="MKRVFAVAFASLLVLAAGCGPSGPSLGAVTGKVTLDDKPLANVS
                     VVFMPVGEGGTASAITDANGNYELGHQAGKGAPVGKNKVSVTTVQQSKSTVDMSNIPS
                     DSPEYAKMAAGGSNAAEYNVQFTEPIPAKYNTQTTLEFDVKSGKQVINLDLKSS"
BASE COUNT          809 a         1340 c         1338 g          737 t
ORIGIN      
        1 agctggagcc gtacaacttt gcgaactatt tggacctggc caaggcctcg gcccagctcg
       61 gcgagcccga agcggccgaa gcgacgctca agctggcgat caccatgcag ccgcaggcgg
      121 ctgacggcta cacggccctg gcccagttct acttggagag cagccgggca aaaaaggccc
      181 gctggtacag ccaggaagcg gtccgccgcc agccgacagc cgagggctat cggctgctgg
      241 cctcgacctg ccgcctgcta ggcgacaccg ccagtgccga ggcggcgctg gctctggccg
      301 gtcagctcga atcggaacgc cgcagcccgc cgcaaaccaa cccgaaccgt gcagcgccgc
      361 ccgccgcggc agggaagcag ccatgatgtc cagtgctcca atcgcccgat cgtggcctgg
      421 ctggatgctc gccgtggccc tactgccggc gctgctgtcg gtgctgctgt cggtgctgct
      481 gtcggcaccg gcccaggcgc agtcgcccgc ttcctgcccg attcgcctga cggacgcgac
      541 ggcggcctcg ggcatcaaat tccgccacac cgacgggggc agcggccagg gctacatcat
      601 tgaagggatg gcatcgggca tcgcgacatt cgattacgac ggcgacgggc tgatcgacat
      661 ctatttcttg agcggcgccg cattgaaagg caccgtcctc gattctccgg ctcgcgacgc
      721 cctgtatcgc aacaacggcg actggacgtt taccgacgtc accgagcaag cgggtttggg
      781 cgacctgcac cacagcctgg gcgtggcggc ggccgactac gacggggacg gggaccagga
      841 cctgtacgtc agcaatttcg gcccgaacgt gctgtatcgc aacaacggcg accggacgtt
      901 taccgacgtg accgagcagg ccggcgtcgg caacggcaac aaggtcggcg cgggatgcag
      961 ctttttcgac atggacggcg acggcgacct ggatctgtat gtcggcaatt acgtcgattt
     1021 cacctatgaa aaccacgtgc cgatcgtcgt caagggacgc catttccagg ccgggccgca
     1081 gtattaccag ccggttgcgg acacgctgta tcgcaacaac ggcgacggga catttaccga
     1141 cgtgagcggc ccctcgggga ttgccgccgt cgccgggccg agcatggcga cgctcgcctt
     1201 cgactacgac gacgacggcg acgcggacat gttcgtctgc aacgacggca agccgaactt
     1261 cctcttcgag aacgacggcc acggcaaatt ccaggaaaag gcgctcgtgg cgggcctggc
     1321 ctataacttc tatggcaagg ccatttccag catgggggtc gattgcggcg acgtggataa
     1381 cgatgggcgg ctcgatttgt tcgtgacttc ctatcaaagc gagatgcccg tgctgttccg
     1441 caacctgggg ggcggcctgt tcgaggacgg gacgagcacc gcccgcatcg ccaccgattt
     1501 tttcgcccac gtcgagtggg gcaccggcca ggtcgatttc gacaacgacg gcgaccgcga
     1561 cctgtttttg gcctgcggcc atttcgaccg catcgagctg ctcgacgacc ggacttcgct
     1621 caaggtgccc aactacgtgc tcatgaacga cggccagggg cggttcaccg acgtttccaa
     1681 acagtgcggc agcgggctgg ccgtcgtcga aagcagccgc ggcgtcgcct tcgacgacct
     1741 ggataacgac ggcgacgtgg acgcggtggt gctcaattcc aacgccgctg gcacactgct
     1801 gcgcaatgag tcgccagccg gcaaccactg gctggagctg cggctgcaag gggggcagag
     1861 gaatttggac ggcattgggg cccgagtgaa ggtggtttcc ggcgaacgtg cgcagatcgc
     1921 agaagtccac agcggccgcg gataccagag ccactatggc acgcggctgc atttcggcct
     1981 ggggcagcag cagcgggtgg accggattga agttcgctgg ccggggggca gcgtcgagca
     2041 cttcccaggg ggcgaacccg atcgattgct ggtgctcaag caggggagcg gaaggcagta
     2101 gcgtgcccct tccccccctg cgctgccaac gtaactagtc aaaaaaacct gacatttcgg
     2161 ttgcttttca gcggttcgca ttctagactc tggggcgctt gtagtgattc ggctattccg
     2221 gccttgcgcc gggaaagcat tcatatcctc tctctttgga ggaacggtta tgtggagacg
     2281 ctggtttcga agcggcttta cgctggtaga gctgctggtc gtgattgcaa tcatcggggt
     2341 attggtggcc ctgctcttgc ccgcggtcca ggccgcccgc gaagcggccc gccggtcgag
     2401 ctgctcgaac aatctgaagc agcacgccct gtcgatgcac aattatcacg acacctacaa
     2461 ggtgtttccc gccctcggca ttcgcccgga gaacaaccag ggcaactttg gcgggacaaa
     2521 caacggcaac tggtacggtt ggctgattgc caccatgccg tatatcgagc agaagccgct
     2581 gtacgacaac atcatgacga gagtcgccga tcctacggtt agcttgccga cggcctggta
     2641 caacctcaac aacaacagcg acccatgggc gaatcagaac tggaaattcg atctgggcgt
     2701 caacatgtgc ccgtcatccc ccaagccgac gattcgcacc gagagcccgg cactgttgag
     2761 ctacaaggtg tgcgtgggcg acgattacca tcaaaaccac ttcctgccca tcgatggccg
     2821 cgacaaccgc ggcatttttc agatggaccg gtggattggc atggaggcaa tcaccgacgg
     2881 caccagccaa acggtgatga tgggcgagtg cgtcatgggc ggccagccga ctgacgtgct
     2941 aggcggcgtg gcggtcaacg tgcaacagtg gtcgccacaa gattgctgga atcgggtgga
     3001 tcccaacaat cgcaaacaac tgctggccag cgccggcgtg cgggctgact tccgcccgac
     3061 cggcggtcgc gcacttgacg gccgtcccta ttacgtcggc ttcgcgaccc tcgtcgcccc
     3121 gaacgggccc acttgtcact ggggtggcgt tgatggcaac gagcatgaag gaacgctctc
     3181 cagcttccat cccggcggcg ggcaagtggc catggccgac ggtcacgtgc agttcatcag
     3241 ccagacgatc aacgccggca atcccacgat tcctgacgac ggggccggtt ggccgagcaa
     3301 taaccgctcc ggcccttcgc cgtacggcgt gtggggcgct ctgggcagca agaacggcac
     3361 cgaggccatt gccaatcaat agcagccaac caataaggcc tcgcgggctt gcccgcggcc
     3421 cggcagaggg gcgcgcgcca ccttgggcgc acgccgtcat ttcgctatcg tcggccagcc
     3481 tccggcgctc ggttcttgtc gaggaagggt gtgctcttat gaaacgggtg tttgctgtgg
     3541 cgttcgcttc gctcttggtc ctcgccgccg gctgcggtcc gagcgggcct tcgctcgggg
     3601 cagtcactgg aaaggtgacg ctcgacgaca agccgctggc caatgtcagc gtggttttta
     3661 tgcctgtcgg cgaaggggga actgcctcgg caattacgga tgccaacggc aactacgagc
     3721 tcggccacca ggccggcaag ggagctcccg tgggcaagaa caaggtatcc gtgacgacgg
     3781 tgcagcagtc caaatcgacg gtggatatgt cgaacattcc gtcggactct cccgaatatg
     3841 ccaagatggc ggccggcggc agcaatgccg ctgaatacaa cgtccagttc accgagccga
     3901 ttcccgccaa gtacaacacg cagacgacgc tcgaattcga cgtcaaatcg ggcaagcagg
     3961 tcatcaacct cgacctgaag tcgtcttaag ccgcgccagg ccgatcggca gatggaaccc
     4021 atgccgcagc tgcgaaccga gtttccctgc tggtcagcat gcgccagtaa ttccgcctga
     4081 gccatttccg catggctggc ggaacgtgac gtttactggg cgaccaccag ttccccatcc
     4141 atgccgatgt cggcctgcgt cgtggtcttg ccgcccggcc agcggaccgt cacccgctcg
     4201 atacttgtcc gctcgcccag gccg
//
LOCUS       DASUGB010000797         5862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_782831, whole genome shotgun sequence.
ACCESSION   DASUGB010000797 DASUGB010000000
VERSION     DASUGB010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782831"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..4041
                     /gene="ccsA"
                     /locus_tag="VFV87_15240"
     CDS             76..4041
                     /gene="ccsA"
                     /locus_tag="VFV87_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5105173.1"
                     /translation="MSAPALPVTNVPAKSLARPAPALTVLDLVNGFMKGLASLKLTVA
                     LMAFGIFVIFVGTLAQTEADIWQVVRDYFHAWVMWVDVNILFPQSFFPHMPHLALPLI
                     PMPGGMTVGVLMVINLLAAHGWRFKIQASGMRLFAGLAVIAAGLAVALLIIWAGHNTQ
                     GFQSKPPFSWEAFWNVFLWVGGVLWLGSLAGYVYYWYSLLTKKSALTIILSVLVNIIL
                     GGGLVFLAGLLGWALYEGWVNGRRPSDEAIRIVWQLLQGGLAGAILLVGCILVFRKRG
                     GIVLLHAGIGLLFFNELFVAYNARERQVFLQEGQTTNFLRDIRTTELAIIDRSDKEND
                     THFVIPRSVLLNNLKANARERAKLIDSPAVEKEMKRLLEERNLTSAELAKDDAKFDQL
                     IDEAIDAAVDPKDRKAVYIDHALLPMKVAIVDYYTNADVRDLKPGEKTPATAGRGLKE
                     AIEKLPAAKGTDMGGGVDLAAAYVKFAEKKTGKDLGTYLLSQLASEQKNPDRFAEKVT
                     LDGKTYQLFLRFKHEYKPYTITLDDVRKDDYVASDTPRNYSSDIRIQDAKAGIDQPVH
                     IKMNDPLRYRGDTFYQSGYHPPAVTGGAEATTLQVVLNRGWMIPYIACMAVVLGMVAH
                     FLISVIRFISRRETEEIAAGDVITAEVIDDQPRIDPRTRRTLPPPKPHRSAADRQWGW
                     SYVILPLITALVFAFYVNSAASVPRAKSGEIDFYRFGQLPVAHLGRVKPLDTLARNTL
                     RAISGNGRETVKVEYEEPSKVPGGKPIEKMRRISAVQWLLEVASGTEASTEYKVIRID
                     SKEVRRLFDLPDRSGYMYSVAELKPQIGRFEKAVEAARKLSAEDLTTEQRRILELDDA
                     LKQYITLVRAFSPPDLPPFPPKEEVEANPGLGRQYIERFSMAVQRAAEEARVTKAPRL
                     IPVELGEKNAPEEVWRPYPVAWSLAYMQHQILGDEPDPTTLAFEEVLSAYRQVADGNG
                     KAADFNTAVTKYEARLRKANPPLWNSNKNGWEAYYNEFSPIYVGIALYVMSFLLGVFG
                     WLFRYKPLNWAAFTMILLTFGLHTLVLIARMYISGRPPVTNLYSSALFIGWGAVLFGL
                     VIEAIFRLGLGNIMAAVLGYATLQVAYFLGAGGDDNIAVMQAVLDTQFWLATHVVCIT
                     LGYAATYAAGLFGLLYVIFGLCTPLLNERSRKELGRMIYGITCFALLFSFYGTVLGGL
                     WADDSWGRFWGWDPKENGALIIVLWNALVLHARWDKMIADRGLAVMAIGGNIVTSWSW
                     FGVNELGIGLHSYGFTEGVLLALGLFVGSQLLLIGLGCLPKSWWWSVQADTERFGPQA
                     A"
     gene            4287..4910
                     /locus_tag="VFV87_15245"
     CDS             4287..4910
                     /locus_tag="VFV87_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX5105174.1"
                     /translation="MAYSLPALPYAYDALEPHIDKRTMEIHHTKHHQAYINNVNTAIA
                     GTDLEKLSVEDLIKDLGKVPDDKRGAVRNNGGGHANHSLFWTIMGPGAGGAPSGDLAA
                     AINSSLGSFDNFKAEFTKAATTRFGSGWAWLVVEGGKLAVGSTANQDSPLMGKATAGI
                     GGTPILGLDVWEHAYYLNYQNRRPDYITAFWNVVNWNEVAKRLAAAK"
     gene            complement(5013..5738)
                     /gene="tsaB"
                     /locus_tag="VFV87_15250"
     CDS             complement(5013..5738)
                     /gene="tsaB"
                     /locus_tag="VFV87_15250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5105175.1"
                     /translation="MLILAFDTSGFGGSVALLEGERVLVERQLDANRRSAQTLAPATA
                     EALSEAGVRPADVRLVATTIGPGSFTGLRVGVTAAKSFAYAARCDVIGLNTLDVIAAQ
                     VPADFLPSPFVGEGSGVRGSDSPDYEIHAILDAQRKELFLARFRPAAAGPERLAEDTI
                     IAAQSWLDPLRPGTIVTGPGLHKLESLLPPGVIAAPQPLREPRAATAGAAAWREYQRG
                     RRDDLWRLAPAYGRPSYAEERRP"
BASE COUNT         1088 a         1860 c         1804 g         1110 t
ORIGIN      
        1 ctggcgaaac gcgaaaacgc ccgctttcaa gaattcgtcc gctccctgcg gctcaagtaa
       61 gaggaccgcc cgcccatgtc cgctccggct ctgcccgtca cgaatgttcc cgccaaatcg
      121 ctggcccggc ccgcgccggc cctgacggta ttggatctgg tcaacgggtt catgaagggc
      181 ctggcttcgc tcaagctaac cgtcgccctg atggcgttcg gcatttttgt gattttcgtt
      241 ggcacgctgg cccagaccga agccgatatc tggcaggtcg tccgcgacta cttccacgcc
      301 tgggtgatgt gggtcgatgt caacatcctg tttcctcagt cgttcttccc acacatgccg
      361 catctcgcac tgccgctcat tcccatgcct ggcggcatga ccgtgggcgt gctgatggtg
      421 atcaacctgc tggccgcgca cggctggcgg ttcaagattc aggcgagtgg catgcggctt
      481 ttcgcgggac tggccgtcat cgccgccggc ctggcggtcg cgctgctgat catctgggct
      541 ggacacaaca cgcaaggctt ccaatcgaag ccgccctttt cgtgggaagc gttctggaat
      601 gtctttttgt gggtgggagg tgtactttgg ctgggctcgc tggcaggtta cgtctactac
      661 tggtattccc tgctgacgaa gaaatccgca ctgacgatca tcctttcggt gctggtcaat
      721 atcattctcg gcggcggcct cgtcttcttg gcgggactgc tcggctgggc cctctatgaa
      781 ggatgggtca acggccggcg tccttccgat gaagcgattc gcatcgtctg gcagctcctg
      841 cagggcggtc tggcaggcgc gatcctgctt gtcggctgca ttctggtctt ccgcaagcgc
      901 ggcggaatcg tgctgctgca cgcggggatc ggcctcttgt tcttcaacga gctgttcgtg
      961 gcctataacg cccgcgaacg gcaggtcttc ctgcaggaag gccagacgac caacttcctc
     1021 cgcgacatcc gcacgaccga gctggcgatc atcgaccgct cggacaaaga gaacgatacg
     1081 cacttcgtca tcccacggag tgtgcttctc aacaatctaa aagcaaacgc gcgcgagcga
     1141 gccaagctga tcgacagccc cgcggtagag aaagaaatga aacgcctcct ggaggagaga
     1201 aatttgacct ctgccgagct ggcgaaggat gatgcgaagt ttgatcagtt gattgatgag
     1261 gcaattgacg cagccgttga tccgaaagat cgaaaagccg tttacatcga tcacgccctt
     1321 ctgccgatga aggtagcgat tgttgactat tacacgaacg ccgacgtgcg ggacctgaag
     1381 cccggcgaaa aaacccccgc caccgccggc cgcggcctca aggaagcgat cgagaagttg
     1441 ccggccgcga aggggaccga tatgggcggc ggcgtcgatc tggccgcagc ttacgtgaaa
     1501 ttcgcggaga aaaagaccgg caaagacctt ggcacttacc tgctctcgca gcttgcttcc
     1561 gagcagaaaa atcccgatcg gtttgccgag aaagtgacgc tggatggcaa aacctaccaa
     1621 ctcttcctgc gcttcaaaca cgagtataag ccgtacacga tcacgctcga cgacgtgcgg
     1681 aaggacgact acgtcgcttc cgacacgccc cgcaactact cgtccgatat ccgcatccag
     1741 gatgccaaag ccggcatcga ccagccggtc cacatcaaga tgaacgaccc gctccgctac
     1801 cggggcgata cgttctatca gagcggctat cacccgccgg ccgtcaccgg gggagcagaa
     1861 gcgaccacgc tgcaagtggt cctcaaccgc ggctggatga ttccatatat cgcctgcatg
     1921 gcggtggtgc tgggcatggt ggcccacttc ctgatttcgg tgattcgctt catcagccgc
     1981 cgcgaaacgg aagaaatcgc cgcgggcgac gtaatcaccg cggaagtgat cgacgaccag
     2041 ccgcggatcg acccccgcac tcggcgcacg ctgccgccgc cgaagcctca ccggtccgct
     2101 gccgaccgcc aatggggctg gtcctacgtg attttgccgc tgatcaccgc gctggtgttc
     2161 gccttctatg tcaattccgc cgccagcgtg ccgcgggcca agtcgggcga aatcgatttc
     2221 tatcgctttg gccagcttcc ggtcgcgcac ctggggcgcg tcaagccgct cgacacgctc
     2281 gcccgcaaca cgctccgcgc gatctccgga aacggccgcg agacggtgaa ggtcgagtac
     2341 gaagaaccgt ccaaggttcc tggcggcaaa ccaatcgaga aaatgaggcg gatttctgcc
     2401 gtccaatggc tcctggaagt cgcctccggt accgaggctt cgaccgagta caaagtcatt
     2461 cgcatcgaca gcaaggaagt ccgccgcctc ttcgacctgc cggaccgttc gggctacatg
     2521 tattcggtcg ccgagctgaa gccgcagatc ggccgcttcg aaaaggccgt cgaagccgcc
     2581 cgcaagctta gcgctgaaga cctgaccacc gaacagcggc gaattctcga gttggacgac
     2641 gcgctcaagc aatacatcac gctcgtccgg gctttcagcc cgcccgattt gccgccgttc
     2701 cctcccaagg aagaggttga agccaatccc ggcctcggcc ggcaatacat cgagcggttc
     2761 agcatggccg tccagcgcgc cgcagaagaa gcgcgagtga ccaaggcccc gcgcctgatt
     2821 ccggtcgagc tgggcgaaaa gaacgctccc gaagaagtct ggcgtcctta tcctgtcgcc
     2881 tggtcgctgg cctacatgca gcaccagatt ttgggcgacg agcccgatcc gacgacgctc
     2941 gccttcgagg aagttctcag cgcctatcgc caggtggctg acggcaacgg caaggctgcg
     3001 gatttcaaca ccgccgtgac caaatacgaa gcccggctgc ggaaggccaa tccgccgctc
     3061 tggaattcca acaagaacgg ctgggaagcg tattacaacg agttctcgcc gatttacgtc
     3121 ggtatcgccc tgtatgtcat gtcgttcctg ctgggcgtct tcggctggtt gttccgctat
     3181 aagccgctga actgggccgc ctttacgatg atcctgctca cgttcggcct gcacacgctc
     3241 gtcctgatcg cccggatgta catctccggc cgcccgcccg tcacgaacct ctattcctcc
     3301 gccctgttca tcggctgggg ggcggtgctg tttggcctgg tgatcgaggc gatcttccgg
     3361 ctcggccttg ggaacatcat ggccgccgtt ctgggctatg ccacgctgca agtcgcctat
     3421 ttcctgggcg ccggcggcga cgacaacatt gccgtcatgc aagctgtgct cgacacacag
     3481 ttctggctgg cgacgcacgt ggtctgcatc acgctgggat atgcggccac ctacgccgcg
     3541 gggctgttcg gtcttttgta cgtgatcttc ggtctctgca cgccgctcct caacgagcgc
     3601 tcgcgcaaag aactggggcg gatgatctac ggcatcactt gctttgcctt gctgttcagc
     3661 ttttatggca ccgtgctggg gggcctctgg gccgacgatt cctggggccg gttctggggc
     3721 tgggatccca aggagaacgg cgccctgatc atcgtcctct ggaatgcgct cgtgctgcac
     3781 gcgcgctggg acaaaatgat tgccgatcgc ggcctggccg tcatggccat cggcggcaac
     3841 atcgtcacaa gctggtcctg gttcggcgtc aacgagctcg gcatcggcct gcacagctac
     3901 ggctttaccg aaggcgtgct gctggcgctg ggcctgttcg tcggctcgca actgctcctg
     3961 atcggcctgg gctgtctgcc caagtcgtgg tggtggagcg tccaagccga caccgaacgg
     4021 ttcgggccgc aggcggcgta gctactcctc tggttcccaa gctccgcttg ggaagagcgc
     4081 gtctggaagc tctgcttcct gtccggcccc ggggcgagtg attctatcgc tcgaagcgga
     4141 gcttcggaga cgtgcgtccc caagcggagc ttgggaacga gaagccccgt ccccgattgt
     4201 cgccggtcga agcgcgcggt atcgtcggct ggagcagtcg attctcttgc gcttcttgcc
     4261 catcaccctc gacaaggagt ctgacgatgg cctattccct tccggccttg ccctatgcct
     4321 atgacgccct ggagccgcac atcgataagc ggacgatgga gatccaccac acgaagcacc
     4381 atcaggcgta catcaacaat gtcaacacgg cgattgccgg gaccgatctc gagaagctga
     4441 gcgtcgagga cttgatcaaa gatctgggta aggttcccga cgacaagcgc ggggcggtcc
     4501 gtaacaacgg cggcggccac gccaatcact cgctcttctg gacgatcatg ggccccggcg
     4561 ccggcggcgc tccctcgggc gatctggcgg cggcgatcaa cagcagcctc ggcagcttcg
     4621 acaatttcaa ggccgaattc accaaagccg cgacgacccg cttcggcagc ggctgggcat
     4681 ggctggtggt cgaggggggc aagctggcgg tcggctcgac ggccaatcag gacagcccgc
     4741 tgatgggcaa ggcgacagcc ggcatcggcg gcacgccgat cctgggcttg gacgtgtggg
     4801 agcacgccta ctatctcaac taccagaacc gccggcccga ctacatcacc gcgttctgga
     4861 acgtggtgaa ctggaacgaa gttgccaaac gcttggccgc ggcgaaataa ccgtaggcaa
     4921 ctcgcggagc gagttggaat gcgagtcaac tgacgcgcga cttcggccgg ccaaagatgt
     4981 cgaccgcttc tcgcggagcg agaagcctac ggtcacggcc ggcgttcttc cgcataactt
     5041 ggccggccgt aggcgggagc gagtctccag aggtcgtcgc gccggcctcg ctggtattcg
     5101 cgccaagccg ccgcacccgc cgtcgcggct cgcggctcgc ggagtggctg tggggccgcg
     5161 attacaccgg gcggcagcag ggattccagc ttgtgcaacc cgggacccgt gacgattgtt
     5221 cccgggcgga gcggatcgag ccaggattgg gcggcgatga ttgtgtcctc ggcgaggcgc
     5281 tcggggccag ccgctgccgg ccgaaagcgg gcgaggaaga gttcttttcg ctgggcgtcg
     5341 aggatggcgt ggatttcgta atccggcgaa tcgctacctc tcacccccga tccctctccc
     5401 acaaagggag aggggagaaa atcggccggc acttgagctg cgatgacgtc gagcgtgttg
     5461 aggccgatta cgtcgcaccg cgcggcgtag gcgaatgact tcgccgcggt gacgccgacg
     5521 cgcagtccgg tgaacgagcc ggggccgatc gtggtggcaa ccaggcgcac gtccgctggg
     5581 cgcactccgg cttcagacag cgcttcagcc gtggccggcg cgagggtctg ggcactgcgg
     5641 cggttggcgt caagttgtcg ctctactagg acgcgctcgc cctcgagcag cgccacgctg
     5701 ccgccaaagc ccgaagtatc gaacgccaga atcagcatgc ctgattttcc gtcaacttag
     5761 atacaaccgg actaacgaca cccgcggacg cagccagatt gcaaagtatt tccagcaggc
     5821 gacttcggac tatgcggcca gccgccgccc gcgaccgctt gt
//
LOCUS       DASUGB010000798         4379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1199354, whole genome shotgun sequence.
ACCESSION   DASUGB010000798 DASUGB010000000
VERSION     DASUGB010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199354"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..2400
                     /gene="ligD"
                     /locus_tag="VFV87_15255"
     CDS             13..2400
                     /gene="ligD"
                     /locus_tag="VFV87_15255"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5105176.1"
                     /translation="MPKGPCLDPSEKRLAVQVEDHPLEYATFEGTIPEGEYGAGEVIV
                     WDRGKWQPPDNPAAQLRRGKLEFELAGEKLQGGWRLVRMTGERSDGGKNWLLMKRRDD
                     AARPLGRHDIVQERPESVKTGRRLEDLKAAPRKARRSSRHSSKGKPRGKGSKRAKQRS
                     PSDPLPGSIDVQLATLASAAPTGPEWLHEIKFDGYRLVARIESGLVQLLTRNHLDWTD
                     RYSAVAEALQEIPAESAILDGEVVAQLESGETSFQALQNALRDRASHKLVYYAFDLLY
                     LDGRDLRSLPLQERKGKLQALLAEGAPPQIQFSGHFADDGQRFWEECCKRGLEGIVSK
                     RRDKPYRAGRSEDWLKIKCVLREELVIGGFTISPAKHRDFGALLVGYFDDGRLTYAGR
                     VGTGFDARTLAGLRRWLDQLETSRSPFAAVPAKERAGIVRWVQPRLIAQVDFGGWTND
                     GILRHPSFQGLREDKSPSEVHRPESLSLPAAPSTSRRRMKKKSSTRRQRSTKAAEHEL
                     PVRLTHPDRLLFPDMGLTKRDLVNYYTAVADWILPHLKGRPLSLLRCPEGQGKACFFQ
                     KHAAAGTPDALQRIDIEEKDETEEYLIANDLPGLLSLAQMSVLEIHPWGSRSDRLEHP
                     DVLIFDLDPGEGVAWSEVVKAGVALRESLESIGLTTFAKLTGGKGLHLVAPLSPRRHD
                     WDEVKSFARSVAEHFAAEQPRQFVATMSKARRRGKIFIDYLRNDRGATAIAPYSTRAK
                     PGAPVACPIGWDELSARVHPNGFNVENLPKRLNSLASDPWDGFFDLKQPLPKFTE"
     gene            2662..3555
                     /locus_tag="VFV87_15260"
     CDS             2662..3555
                     /locus_tag="VFV87_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5105177.1"
                     /translation="MAKRKQIATKQPKQKASRQGGPRPRAGWSGNLTFGLVTFPVEAF
                     NAVSREQGEIHFHLLHKGCHRRIKYQKVCPVHGEISNDDIVSGYEYQKDHYVEIAKEE
                     LDELRTERERSLTIDTFIGCDAIDLRFFDGRMYYLAPDGAAAEEPYAVMTEALAREKK
                     CGVGQMIFSSKEQLALIRPQDGILHLAMLNYDEEIRDPAETVGPIEAPSGMARKVELA
                     QSLIDSWTADDFDFTHYDDPYRERVQALIEAKLQGRTFTAPKEETAPPVINLMDALKK
                     SLSTAPTGRKKTKSKAKRRSA"
     gene            complement(3561..>4379)
                     /locus_tag="VFV87_15265"
     CDS             complement(3561..>4379)
                     /locus_tag="VFV87_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105178.1"
                     /translation="ELYYSGEQKLSQYKNGFFQKLSLSAGWIGDSNSPTDLGVTEIES
                     FVTVALPAPIREWPLLITPGYNMIFLSGPTVTDLPPRLNTAYVDFMWLPNIINRYTLL
                     LSVAPSVYSDFQAHDPDMFRVTGKGLLIYDWSPGYLQVIAGLLYLGRDDIMILPAGGV
                     IWTPAPWAHYELLFPKPKLALRLNVGQGYEDWIFTTAEFGGNTWSIERDSGMDDKVTY
                     LDYRILLGMERKLNGGAGYRLEGGYVFGRQVTFASGNGDFDPQNSFLLRGAITY"
BASE COUNT          900 a         1316 c         1369 g          794 t
ORIGIN      
        1 aagagttggg ccgtgccgaa ggggccatgc ctggacccat cggagaagcg tttggcagtt
       61 caagttgagg atcatccgtt ggagtatgcc acattcgagg gcacgatccc ggaaggcgaa
      121 tacggtgcgg gtgaagtcat cgtctgggac cgcggcaagt ggcagccgcc ggacaacccc
      181 gcggcgcaac tgcgccgcgg caagctcgaa tttgagctcg cgggcgagaa gctgcagggc
      241 ggctggcggc tggtgcggat gacgggcgaa agatcggacg gcggcaaaaa ctggctcctt
      301 atgaagcgcc gcgatgatgc cgcccggccg ctcggcaggc acgacattgt gcaggagcgg
      361 ccggagagcg tgaaaaccgg tcgccggttg gaagatctga aggcggcgcc gcggaaagcc
      421 aggcgaagct cgcgtcattc ctccaagggc aagccgcgcg gaaagggttc gaagcgcgcg
      481 aagcaacgct cacccagcga ccctctgccg ggatccatcg atgtgcagct cgccacgctc
      541 gcgtccgccg caccgaccgg acccgagtgg ctgcacgaaa tcaagttcga cggctatcgg
      601 ttggtggcgc gcatcgaaag cggcctcgtg caactgctga cacgaaacca tctcgactgg
      661 accgaccgct attcggcagt ggccgaagcc ctgcaggaaa ttccggctga atcggccatt
      721 ctggatggtg aagtagtcgc gcagctcgaa agcggtgaga cgagctttca ggcgctacaa
      781 aacgccctgc gcgatcgcgc ctcgcacaag ctggtctatt acgcgtttga cctcctctac
      841 ctggatggac gcgacctccg ctcgttgccg ctgcaagagc gcaaaggcaa gttgcaggcg
      901 ctcttggccg aaggggcgcc gccccagatt cagttcagcg gccacttcgc tgacgatggg
      961 cagcgattct gggaagaatg ctgcaagcgc gggctcgaag ggatcgtctc caaacgtcgc
     1021 gataagccct accgcgcggg acgctccgag gactggctga agatcaagtg cgtcttgcgc
     1081 gaggagctgg tgatcggcgg gtttacgatc tcgccggcca aacatcgcga ttttggggcg
     1141 ctgctcgtgg gctatttcga cgacggccgg ctgacctatg ccggccgcgt ggggacgggt
     1201 ttcgacgcgc ggacattggc cgggctgcgc cggtggctgg atcagctcga aaccagccgc
     1261 tccccttttg ccgcggtccc agccaaggaa cgagccggca ttgtgcgttg ggtccagccg
     1321 cggcttatcg cccaggtcga ttttggcggc tggacgaacg acggcatcct gcggcatcct
     1381 tcgtttcagg gtcttcgcga ggacaagtcg ccaagcgagg tccatcgccc ggaatcgttg
     1441 tcgttgccag ccgcaccctc caccagcagg cgtcgcatga agaaaaagtc ctcgactcgt
     1501 cgccaacgtt ccacgaaagc tgccgaacac gagctacccg tccgcctgac ccatccggac
     1561 aggctgcttt tccctgacat ggggctgacg aagcgtgatc tcgtcaacta ctacacggct
     1621 gttgccgact ggattctgcc gcacttgaaa ggccggccgc tcagcctgtt gcgttgtccg
     1681 gaaggccagg ggaaagcctg cttctttcag aagcacgccg ccgccggcac gcccgatgca
     1741 ctgcagcgca tcgacatcga agagaaggac gagaccgagg aatatcttat cgccaacgac
     1801 ctgccagggc tgctttcgct cgcgcagatg agcgttttgg agattcaccc gtggggatcg
     1861 cggagcgatc ggctggagca tcccgacgtg ctgatcttcg atctggatcc aggcgaaggc
     1921 gtggcctgga gcgaagtcgt aaaagcaggc gtcgcgctcc gcgagtcgct ggaatcgatc
     1981 gggctgacaa ccttcgcgaa actgacaggc ggcaagggac tgcatttggt tgcgccgctt
     2041 tcgccgcggc ggcacgattg ggacgaagtg aagagcttcg cccgttccgt cgccgagcac
     2101 ttcgctgccg agcagccgcg gcaatttgtg gcgaccatgt ccaaggcccg gcgccgcggc
     2161 aagatcttca tcgattatct gcgcaacgac cggggagcaa ccgcgatcgc tccctattcc
     2221 acgcgagcca aaccgggcgc gcctgtggcc tgtcctatcg gttgggacga gttgtcggcc
     2281 cgcgtgcatc cgaacggctt taatgtcgag aaccttccca aacgcctgaa ctcgcttgca
     2341 agcgatccct gggacggatt tttcgacctc aaacagccgt tgccgaaatt cactgaatga
     2401 cgtggcttca tggagtttcg atgctcaggt gtaagttcga gcaaggtttt gacttcacgg
     2461 cgtaaatcgg ggacgcgagc aagtgccgga agaagagcgc tgagataagc gatttcgttc
     2521 ttcattaggc aggtgcccgg cgacactttg tcgcgtcaac tcccgtcgca gttgctaagc
     2581 gctctgcctc tattgggggg cagatggatc ctgccttgag tgctagcact gctggcacgc
     2641 ggtctgcaat tactgcgacg catggccaaa cgaaagcaga tcgcgaccaa gcagccgaag
     2701 caaaaagcca gtcgccaagg cggtccacgg ccgcgcgccg gctggagcgg gaaccttacc
     2761 tttggcctcg tcaccttccc ggtcgaagcc ttcaacgccg tcagccgtga gcaaggcgaa
     2821 atccactttc atcttcttca caaaggctgc caccgccgta tcaagtacca aaaggtgtgt
     2881 ccggtacacg gggagattag caacgacgac atcgtctcgg ggtacgaata ccagaaggat
     2941 cactatgtcg agattgccaa ggaggagctg gacgagctgc gaaccgagcg cgagcggtcg
     3001 ctgacgatcg acacgttcat tggatgtgac gcgatcgacc tgcgtttttt cgacggccgc
     3061 atgtactacc tggcacccga tggcgctgcc gccgaagagc cctatgccgt gatgaccgaa
     3121 gccctggctc gcgagaaaaa gtgtggcgtc ggccagatga tcttctcatc gaaggagcaa
     3181 ctggcgctca tccgcccaca agacggcatc ctgcacctgg cgatgctcaa ctacgacgag
     3241 gagattcgcg accccgccga aacggtcggc ccgatcgagg ccccctccgg aatggcgcgc
     3301 aaggtcgaat tggctcaatc gctgatcgac tcatggaccg ccgacgattt cgatttcacg
     3361 cattacgacg atccttatcg cgagcgcgtg caggcactaa ttgaggccaa gctccagggt
     3421 cgtactttta cggctcccaa agaggaaact gccccaccgg tcatcaatct tatggatgcc
     3481 ctgaagaaga gcctttcgac ggctcccact ggccgcaaaa aaaccaagtc gaaggccaag
     3541 cgccgctcgg catagcagcc tcaataggta atcgcgccgc ggagaagaaa cgagttctgc
     3601 gggtcgaaat cgccgttgcc gctggcgaaa gtcacttgcc ggccgaagac gtagccccct
     3661 tccagccggt agcccgcgcc gccgttgagc tttcgttcca taccgagcaa gatgcggtaa
     3721 tcgagatagg tcactttatc atccatcccg ctgtcgcgct cgatcgacca ggtgttgccg
     3781 ccgaactcgg ccgtcgtgaa gatccaatcc tcgtagccct ggccgacgtt cagccgcagc
     3841 gccagcttgg gtttcggaaa gagcagttca tagtgcgccc acggcgcggg cgtccagatg
     3901 acaccgccgg ccggcaggat catgatgtcg tcgcggccta ggtagagcag gcccgcaatc
     3961 acttgcagat agccgggcga ccagtcataa atcagcagtc ccttgcccgt cacgcggaac
     4021 atgtccggat cgtgggcctg gaaatcggaa tagacgctgg gagccactga caagagcagc
     4081 gtgtagcggt tgatgatgtt cggcagccac atgaaatcga cgtacgccgt gttcaggcgc
     4141 ggcggcaggt cggtgacggt tgggccgctg aggaaaatca tgttgtagcc gggcgtgatg
     4201 aggagcggcc attcgcggat cggcgccggc agggcgaccg tgacgaacga ttcgatctcc
     4261 gtgactccca ggtcagtcgg gctattcgaa tcgccaatcc agccggcgct gagcgacagc
     4321 ttctgaaaga agccgttttt gtactgagac agcttttgct cgccggaata gtagagctc
//
LOCUS       DASUGB010000799         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1893612, whole genome shotgun sequence.
ACCESSION   DASUGB010000799 DASUGB010000000
VERSION     DASUGB010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893612"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1225..2595)
                     /locus_tag="VFV87_15270"
     CDS             complement(1225..2595)
                     /locus_tag="VFV87_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5105179.1"
                     /translation="MPEHRTRIRTTVRPARLAVLFLGAAAALVTGGSFSASELRQSPI
                     VKAVQDAEPSVVSIHGRKTVRPEPVSYGQAESPRQVNGMGTGIVIDPRGYILTNFHVV
                     DGVSNIQVSLSDNRPAIARLIANDPKTDLAVIKIDAKDPLPVIRFGTSCDLMKGEAVI
                     AIGNAYGYEHTVTRGIISALHRNVQVSDDQKYNDLIQSDASINPGNSGGPLMNIDGEV
                     IGINVAVRVGAQGIGFAIPIDEALEVAARLMNVEKLEQTSHGIAGRTVCEPTRRQFVV
                     TGVREESPAAKSGVKAGDVITAIGERKIERALDVELAFLGRTSGEEVTIETTRDGLPQ
                     SSAIATSPITRGLKPALTDRVWTQLGLRLSVMDSAEFQRLGTRYRGGLTVLDVRSGSP
                     AAQQGIRRGDVLVGMHVWETISLENMAYVLDREDLARLDPVVFYIVRGSDTLYGHMQL
                     AKRTVP"
     gene            complement(2772..3209)
                     /locus_tag="VFV87_15275"
     CDS             complement(2772..3209)
                     /locus_tag="VFV87_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105180.1"
                     /translation="MLARFHLAILTSALASAWLGGCCCPPISCRYGEPGCPAGPLAPS
                     CGCGAGCTDCGCGQGASACACGDGHAMHHGLGAHFPLLARGHLHHEPVPTEQKADYVS
                     PTAKYHPVPTRPVFEPQPDYPPPYLLPGPEPRPRLHESHAIIR"
     gene            complement(3311..>3502)
                     /locus_tag="VFV87_15280"
     CDS             complement(3311..>3502)
                     /locus_tag="VFV87_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105181.1"
                     /translation="VVFVATIVEGLRLPLRDWPAWKYWPIYLLVPVVVFLLLQLLQLA
                     VKRDESDLRRLIQGEAPGK"
BASE COUNT          593 a         1031 c         1273 g          605 t
ORIGIN      
        1 cgctcgtcgc cgagcgtttc gccggccagc cctgcggcca cttcgccatg cagcgccgcg
       61 agcgagccct cgcggcagcg gctgcctaat cacggtcggc tcgcgaacca agcatcgctt
      121 ggggtggctg ggttttgcga ggcataaccc agtgaatcgc caggcatgct tccgcgctcc
      181 ctgtggctga tgcccgacgt cggctgggtc atgcttcaag acccagccac tccgccgctg
      241 tagaatcggg ctcatgcgat gcaatcaatg cggacgcgac tttccgtgga cggaggcgga
      301 acacgacacg cacagtgaga cgatcggcag gtacagcgac ggaccgaagt tgttaacaat
      361 gtgtccagcg tgcgcagcca cccgcagggg aatgctgtgg ttctggctgg tcgctggcct
      421 tgccgcggcc atcgcagcgg tgattgccgt cgccaatctt taactctggc tcaggtagcg
      481 ctcggcgagc tggaggagca tgagcgccgg cgaaatatgg gtttgtacgc caaacgcccg
      541 tatggacctg acgtgtcctt ttcatttggg gtgcggaaaa taacttacgg cgagtggcct
      601 gggcgcaagt ggtagtgcgg ctgggggttg gggcgaggcg ggtcggcgca aggggggctg
      661 gcgcggaatt ctggccggaa tttgcttgga gcgcagggaa cgccatggga tattctggac
      721 agtgtgtaca tccgattcga taaggaagcg ggggcgtgag cctcccggtg cggggggggc
      781 ttgcgtgccc tcgctccaca cacctgtccg gtgggtcggg tgcatgaggc cgccgcggcc
      841 aaaatgtaca gcagggggac gacccggcgc gatctgtagt gcaagcggcc agcaacccgc
      901 ccgcgttctg gcgaacgcgg ctacgggtgg gccggttctg gtcggacggg ttggaaaccc
      961 gtcctacggc gaaaagcgca acagcgcaac atcctcattt ggggggtgtt gcgcggcgca
     1021 aacgattgaa ctaagaggag ttaggagaat gttgcgcgga gagcgggggc gtgaaaaaag
     1081 tgcggaaagt tgcgcgttgc gcttccgggt tgcgcgaggc gggagcctcg ggagcgttcc
     1141 gtcccaggcg gagcctggga cggaggtgta atgaggtggg cacagcccac cctacaagat
     1201 ttgtctccag tcagctcgcc aacgttaggg cacggtgcgc ttggcgagct gcatgtggcc
     1261 gtagagggtg tcgctgccgc ggacgatgta gaagacgacg gggtcgaggc gggcgaggtc
     1321 ttcgcggtcg aggacgtagg ccatgttctc cagcgagatc gtttcccaga cgtgcatgcc
     1381 gaccagcacg tcgccgcggc ggatgccttg ctgggcggcg gggctgccgc tgcggacgtc
     1441 gagcaccgtc aggccgccgc gataccgcgt ccccagccgc tggaactcgg ccgaatccat
     1501 caccgagagg cgcaggccga gctgggtcca gacgcggtcg gtgagcgcgg gcttgaggcc
     1561 gcgggtgatc gggctcgtgg cgatggccga gctttgcggc aggccgtcgc gggtggtttc
     1621 gatcgtgact tcctcgcccg aggtacggcc caggaaggcc agctcgacat ccagcgcccg
     1681 ctcaatcttc cgctcgccga tggcggtgat cacgtcgccc gctttgacgc ccgacttggc
     1741 ggctgggctc tcctcgcgga cgccggtcac gacgaactgc ctccgcgtgg gctcgcagac
     1801 cgtccgcccc gcgatgccgt ggctcgtttg ctcgagcttc tcgacgttca tcaggcgggc
     1861 ggcgacttcc agggcttcgt cgatcggaat ggcaaaaccg atgccctggg cgccgacgcg
     1921 gacggcgacg ttgatgccga tcacctcgcc gtcgatgttc atcaggggcc cgccgctgtt
     1981 gccggggttg atgctggcgt cgctttggat caggtcgttg tatttctggt cgtcgctgac
     2041 ctgcacgttg cggtgcagtg cgctgatgat gccgcgggtg acggtgtgct cgtagccata
     2101 ggcgttgccg atggcgatca cggcctcgcc tttcatcaga tcgcacgaag tgccgaagcg
     2161 aatgaccggc agcggatcct tggcgtcgat cttgatcacg gccagatcgg tcttgggatc
     2221 gttggcgatc agccgggcga tggcgggccg gttgtcggag agcgaaacct ggatattgct
     2281 gacgccatcg acgacgtgga aattcgtcag gatatagccg cgggggtcga tcacgattcc
     2341 cgtgcccatg ccgttgacct ggcgcgggct ttcggcctgg ccgtaggaga ccggctcggg
     2401 gcggacggtc ttccggccgt gaatgctgac gaccgacggc tcggcatcct ggaccgcttt
     2461 gacgatgggc gattggcgca gctcggaggc ggagaacgag ccgccggtga caagcgcggc
     2521 ggctgctccg aggaagagca cggccagtcg agcgggtcgg acagtggtgc ggatgcgcgt
     2581 gcggtgctcg ggcatgtatg aactcggcgg gcctgggtcg tcgagcgtgt cgcctggtgc
     2641 ggactgcgca cggcggcagg gcgggtagcc gggggcagtc cgaagttaga tcggcgctgg
     2701 tttgcaggcc gcttcagggg gggccggacg cgcggcagat tccgcccaaa ttcacgcgca
     2761 gcgggcgcgt gctagcggat gatggcgtga ctttcgtgaa gtcgaggacg cggctcaggg
     2821 ccgggcagca aatagggcgg aggataatcc ggctgtggct cgaagacggg gcgcgtcggc
     2881 acgggatgat atttcgccgt tgggctcacg tagtcggcct tctgctcggt cggaaccggc
     2941 tcgtggtgca aatggccgcg agctagcagc gggaaatggg cgccaagccc gtggtgcatg
     3001 gcgtggccgt caccacaggc gcaggcggac gcgccttgtc cgcagccgca atccgtgcag
     3061 ccggcgccgc agccacagct tggggcgaga ggtccagccg ggcagcccgg ctcgccatag
     3121 cggcaggaga tcggcgggca gcagcagccg ccgagccatg cgctggccag tgcggaggtc
     3181 agaatcgcaa gatgaaaacg cgccagcatg ccagcactcg gttgctgcga aagagcggag
     3241 ttgatcgcgc acgtcatcgg cagaaagtgc cggcaaactt caaccaatcg gcataaccat
     3301 gaaagcttcg ctatttcccc ggggcttccc cctgaatcag ccgccgcagg tccgattcgt
     3361 cccgctttac cgcgagctgc aagagctgca ggagcaggaa cacaaccacg ggaacgagca
     3421 gatagatggg ccagtacttc cacgccggcc aatcgcgcag cggcaggcgc agcccttcga
     3481 cgatcgttgc cacgaacacc ac
//
LOCUS       DASUGB010000800         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_506012, whole genome shotgun sequence.
ACCESSION   DASUGB010000800 DASUGB010000000
VERSION     DASUGB010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506012"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFV87_15285"
     CDS             <1..561
                     /locus_tag="VFV87_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5105182.1"
                     /translation="GGQKQRLAVACALVGDPQLLFLDEPTTGLDPQSRRQLWEIIHQF
                     RTSGRTVMLTTHYMDEAERLCDRVAVVDHGKVIALGTPAKLIAELGGEHVIDFALAAG
                     SAAADEAELSRLPSVRAVRCDAERYTLSVAQPHIALPALLSLLTTQGRPLAHLTTRHA
                     TLEDVFVSLTGRHLEEPQVEEPKNGK"
     gene            551..1723
                     /locus_tag="VFV87_15290"
     CDS             551..1723
                     /locus_tag="VFV87_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105183.1"
                     /translation="MANEPDRGAAAEPQGKSAAAPPRVYWPIWHLALSRLRLFLREPS
                     AIFWVYGFPLIMITALGVAFREDPTEHIAVDLIQSRDQWSRSASNLPIEEAPLKTIPP
                     AVASLRDALAADSRFQITTQPSENWRKRLQAGKTDLVIEVLPSGTSSPFQFWDEPRRA
                     ESRLARHAVEAALLRSEQNASPLEVNHLQEPGSRYIDFLLPGMLGMSLMGGGMWGVGF
                     VIVDMRVRKLLKRFLATPMRRSDFLLAIMFSRLAFTLADIAFLLLIGYLAFGVRCYGS
                     YLDLAAATLLGGVSFCGIGLLVASRAETLETASGLMNLVMLPMWILSGVFFSSERFPA
                     EIQPLLNLLPLTALNQLLRGIMLEGSSIARFLPQVAILAAYGAITFILALRIFRWR"
     gene            1723..>2335
                     /locus_tag="VFV87_15295"
     CDS             1723..>2335
                     /locus_tag="VFV87_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX5105184.1"
                     /translation="MSVATAPLVGRLAPSPTGAQHVGNARTYLLAWLSIRSRGGRVIL
                     RMEDIDSPRVKRGAAEQAIEDLLWLGLDWDEGPDIGGPHAPYTQTLRLPLYQQALEQL
                     KAAERVYPCICTRSDVAAAASAPHVGQEGPIYPGTCSHRRGADGVSLTRSVSEGPPFV
                     WRLRTTNQPRHFNDLIAGPQSCNVAAELGDFVVAKADDSPAYQL"
BASE COUNT          378 a          855 c          672 g          430 t
ORIGIN      
        1 ggcgggcaaa agcagcggct ggcggtggcc tgtgccctgg tgggcgatcc gcagctcctg
       61 tttctcgacg agccgacgac cgggctggat ccacagtcgc ggcggcaact gtgggagatc
      121 attcaccaat ttcgcaccag cggccgcacc gtcatgctca ccacgcatta catggacgag
      181 gccgagcggc tctgcgaccg ggtggcggtc gtcgatcacg gcaaggtcat cgccctgggc
      241 acgcccgcca agctgatcgc cgagttgggg ggcgaacacg tcatcgactt cgctctggcc
      301 gcaggaagtg ccgcggccga tgaagcggag ctatcccgcc tcccgtcggt ccgcgccgtg
      361 cgctgcgacg ccgagcggta cacgctctcg gtcgcccagc cgcacatcgc cctgccggcg
      421 cttttgtcgc tgctcacgac tcaggggcgg ccgctggccc atctgacgac gcggcacgcg
      481 accttggagg acgtcttcgt ctcgctcacc ggccgccatt tggaggagcc gcaagtcgag
      541 gagccgaaaa atggcaaatg agccggaccg cggggcggcc gccgagccgc agggcaagtc
      601 agccgcggcc ccgccgcgcg tttattggcc gatctggcac ttggcgctgt cgcggctgcg
      661 gctcttcctc cgcgagccgt cggcgatttt ttgggtctat ggctttccgc tcatcatgat
      721 cacggccctc ggcgtcgcct ttcgcgagga ccccaccgag cacatcgccg tcgatctcat
      781 ccaaagccgc gaccagtggt cgcggtccgc ttcgaatcta ccgattgagg aagccccact
      841 caaaaccatt ccacccgcag ttgcttcact tcgcgatgcc ctcgctgccg actcgcgttt
      901 ccagatcacc acgcagccca gcgaaaactg gcgcaaacgc ctccaggccg gcaagaccga
      961 cctcgtcatc gaagtcctgc ccagtggcac tagttcgcca ttccagtttt gggacgagcc
     1021 gcgccgggcc gaaagccgcc tggcccgcca cgccgtcgaa gccgcgctcc ttcgcagcga
     1081 acaaaacgcc tcgcccttgg aagtgaatca cttacaagaa cccggctcac ggtacatcga
     1141 cttcctgctc cccggcatgc tcggcatgag cctgatgggc ggcggcatgt ggggcgtcgg
     1201 ctttgtcatc gtcgatatgc gcgtccgcaa gctcctcaag cgtttccttg cgactcccat
     1261 gcggcgcagc gatttcctgc tcgccatcat gttcagccgg ctcgccttca cgctcgccga
     1321 catcgcgttc ttgctcctca tcggctacct cgcctttggc gtgcggtgct atggcagcta
     1381 tctcgacctc gcggcggcca cgctgctcgg cggcgtcagc ttctgcggca tcggcttgct
     1441 cgttgccagc cgcgccgaaa cgctcgaaac ggcctcggga ctcatgaacc tcgtcatgct
     1501 gccgatgtgg attctctccg gcgtgttctt ctcctccgaa cgtttccccg ccgaaatcca
     1561 gccgcttctg aacctgcttc ccctgaccgc gctcaaccaa ctgctccgcg gcatcatgct
     1621 cgaaggctcc tcaatcgcca ggttccttcc gcaagtcgcc atcctcgccg cctacggtgc
     1681 catcaccttt atcctggccc tccgcatttt ccgctggcga tgatgagcgt agccaccgca
     1741 ccactcgtcg gccgtctggc cccctcgccg accggcgcgc agcacgtggg caatgcgcgc
     1801 acctatttat tggcatggct ctcgatccgt tcccgcggcg gacgagtaat cctgcgaatg
     1861 gaggacatcg attcgccgcg ggtgaagcgc ggcgcagccg aacaggcgat cgaagatttg
     1921 ctctggctgg ggctcgattg ggacgaaggc ccggatattg gcggcccgca tgcaccatat
     1981 acccagaccc tgcgactgcc gctctaccag caagctctcg agcaattgaa agccgccgag
     2041 cgcgtctatc cctgcatctg cacccgctcc gatgtcgccg ccgcggccag cgccccacac
     2101 gtcggccagg aaggtcccat ctatcccggc acctgcagcc atcgccgtgg cgccgacggg
     2161 gtttcattaa cccgaagcgt cagcgaggga cctcctttcg tctggcgtct ccgcacgacc
     2221 aaccagcccc gccacttcaa cgacctcatc gccggccccc aatcctgcaa cgtcgccgcc
     2281 gaactcggcg acttcgtcgt cgccaaagcc gacgactcgc cggcgtatca actgg
//
LOCUS       DASUGB010000801         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2033594, whole genome shotgun sequence.
ACCESSION   DASUGB010000801 DASUGB010000000
VERSION     DASUGB010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033594"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="leuD"
                     /locus_tag="VFV87_15300"
     CDS             <1..484
                     /gene="leuD"
                     /locus_tag="VFV87_15300"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5105185.1"
                     /translation="GPFLFFDWRYFDDGTTPRPEFELNQPVVQGASVLLARRNFGCGS
                     SREHAVWAIENYGFKAVIAPSFADIFYNNCFKNGVLPIKLSEEQVEDLFQRASKPGYK
                     LTIDLNSCRITDDSGLSLAFTVDESRRHCLLNGLDDIALTLQQEDKIAAYERSRGMAV
                     "
     gene            693..1706
                     /locus_tag="VFV87_15305"
     CDS             693..1706
                     /locus_tag="VFV87_15305"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5105186.1"
                     /translation="MPRLIPAAGLLASLCLTAGTWAAEFEIGKVPAGLKPLPVPTENP
                     LTAEKIELGKQLYFDPRLSRDDTISCASCHDPKKGWSNGAAFATGVRGQVGGRSAPTI
                     INAAYSDLQFWDGRALRLEGQALGPIQNPIEMDLTLDEVVTKLNKIAGYREQFQRVFN
                     TNVTSEGMAKAIASFERTVLSGNAPYDRFKAGDKMALSEAAQRGMKVFFGKGHCSACH
                     VGPNFSDAAFHNIGVGMEKEKPDAGRHDVTKLLGDKGTFKTPTLREIARSAPYMHNGS
                     HQTLEDVVTYYDKGGFANPQLDEEIFPLKLTAAEKADLVTFLKEGLSSPEYPDITPPA
                     LPE"
     gene            1816..2841
                     /locus_tag="VFV87_15310"
     CDS             1816..2841
                     /locus_tag="VFV87_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105187.1"
                     /translation="MKYALASLGFAALLGACLIAIAADDAKVEEVVGGLSNPCGIAIQ
                     PGTGQVFVSDSATGRVCRIVDGKLEDVIAGSPKDVYGKGPFYDIGPLGLVFTDPNTLV
                     VGDGGFKDGEEYLRVFTVPEGGKPALDYEKDAKKLGPLEKTDDLVGEGNLYGLAATKT
                     AVYVTCNGDDTKGWVSKVDIDGSNFGKLERYIATKEQVEVDAPVGITISSRGEIVVGQ
                     MGEVNKPKDSLLTFYSAKTGKKILNLETGLYDIAGLAYSPKTGLLYAVDFAWMAPEEG
                     GLFRLDAENKDGAQSLKAVKIASLDKPTALAFAPDGTLYVTVVGPKGAEENAPKEGKV
                     LKIAPGL"
     gene            complement(2944..>3936)
                     /gene="bioB"
                     /locus_tag="VFV87_15315"
     CDS             complement(2944..>3936)
                     /gene="bioB"
                     /locus_tag="VFV87_15315"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5105188.1"
                     /translation="DWTCLAERVLDDHRVTPNEALAMLRAPDDELLAILDAAFRVRRR
                     FFGKTVQLYFLMNAKSGLCPEDCHYCSQSKVSTADIPQYNLLSREKLLDGARVAAERG
                     SKTYCIVISARGPSERELHAVETIVPEIKAKYDLEVCACLGLLTSEQAGRLAACGVNK
                     VNHNLNTSPEFYEQICTTHTYGDRVETLKAVRQAGLQLCSGGIIGMGERDEDVVRMAL
                     ELRALEVESIPLNFLIDIDGTPLQRMSRLTPPYCLKALAMFRFVNPASEIRIAGGREL
                     HLGSLQALGLYAANSMFVGDYLTTKGQAPEADYKMIEDLGFTVTRHEAVPAGHS"
BASE COUNT          789 a         1223 c         1228 g          696 t
ORIGIN      
        1 tgggccgttt ttatttttcg attggcggta cttcgacgac ggcacaacgc cgcggccgga
       61 gtttgagctg aatcagcctg tcgttcaagg agcgtcggtc ctgctggcgc ggcggaactt
      121 cggctgcggc tccagccgcg agcatgcggt ctgggcgatc gagaactacg gcttcaaggc
      181 ggtgatcgcg ccgagctttg ccgacatctt ctacaacaac tgtttcaaga acggcgtcct
      241 gccgatcaag ctcagcgagg agcaggtcga agacctgttc cagcgagcga gcaagcccgg
      301 ctacaagctg acgatcgacc tgaactcctg ccgcatcacc gacgattcag gcctgtcgct
      361 cgcgttcacc gtcgatgaat cccgccggca ttgcctgctg aacggcctgg acgacatcgc
      421 cctcacgctc cagcaggaag acaaaatcgc cgcctacgaa cgcagccgcg ggatggccgt
      481 gtagcggtgt agggtgggct gtgcccacca tcgacgctca gcaacaaatc ggtgggcgca
      541 gcccacccta cggctgccga ggccgtcagg acctacgaac aggccgtcac gacctacttg
      601 ccgcaggccg tcggtgggac aagaatgtcg tttcaacatc cattgccgcc ccgcgtgtaa
      661 caccgttgcg ttaacctgcc aggagttgcc tcatgccccg cctgattccc gccgccggcc
      721 tgctggcgag cctgtgcctg actgccggca catgggctgc cgaatttgag atcggcaaag
      781 tgcccgccgg tctcaagccc ttgccagtcc cgactgaaaa tccgctaacg gctgaaaaga
      841 tcgagctcgg caagcagctc tatttcgatc cgcggctgtc gcgggacgac acgattagct
      901 gcgcgagctg ccacgatcca aagaagggct ggtctaacgg cgcggcgttc gccaccggcg
      961 tccgagggca ggtcggcgga cgcagcgccc cgacgatcat caacgcggct tattccgacc
     1021 tgcagttctg ggacggccgg gcgctccgcc tggaaggcca ggccctcggt ccgatccaga
     1081 atccgatcga aatggacctg acgctcgacg aagtcgtgac gaagctcaac aagatcgccg
     1141 gctatcgcga gcagtttcaa agggtcttca ataccaacgt gaccagcgaa ggcatggcca
     1201 aggccatcgc ctcgtttgag aggacggtcc tctcgggcaa tgccccgtac gaccgattca
     1261 aggcgggcga caaaatggcc ctctccgagg cagcccagcg ggggatgaag gtcttcttcg
     1321 gcaaagggca ctgctcggcc tgccatgtgg gaccgaattt ctctgacgcc gcgttccaca
     1381 atatcggtgt ggggatggaa aaggagaagc ccgatgccgg ccgccacgac gtgacgaagc
     1441 tgctgggcga caaaggcacc ttcaaaacgc ctacgctccg cgagatcgca cgctccgccc
     1501 cgtacatgca caacggcagc caccagaccc tggaagacgt ggtgacctac tacgacaagg
     1561 gcgggtttgc caatccgcaa cttgacgagg aaatctttcc gctcaagcta actgccgcgg
     1621 agaaagccga cctcgttaca ttcctgaaag agggcctcag cagcccggaa tatcccgaca
     1681 tcaccccacc ggcgctgccg gaataggtac actgcttgca cacacccgaa gggtctcctg
     1741 gcccgtccgg cttcggctcg gcttgagacg cggtttcgcc ctttagaacc aataacacct
     1801 ttggagatgc gaccaatgaa atacgccctc gccagtctcg ggtttgctgc cctgcttggc
     1861 gcctgcttga tcgcgatcgc ggccgacgac gccaaggtcg aagaggtcgt cggcggcctg
     1921 tcgaatccct gcggaatcgc gatccagccg ggcaccggcc aggtctttgt ctccgacagc
     1981 gcgaccggcc gcgtctgccg tattgtcgac ggcaagttgg aggacgtgat cgccggctcg
     2041 ccgaaggacg tttatggcaa gggcccgttc tatgatatcg gcccgctggg actggtgttc
     2101 accgatccga atacactcgt tgttggcgac ggcggtttca aggacggcga ggagtacctg
     2161 cgcgtcttta cggttcccga agggggcaag ccggcgctcg actatgaaaa ggatgcgaag
     2221 aaactcggcc cgctggaaaa gacggacgac ctggtcggcg aaggcaatct ctatggcttg
     2281 gccgccacca agaccgcggt ctatgtgact tgcaacggcg acgacacgaa gggttgggtg
     2341 tcgaaagtcg acattgacgg ctcaaacttc ggcaagctgg agcgatacat cgccaccaag
     2401 gagcaagttg aggtggatgc cccggtaggc atcaccatca gctcgcgcgg cgagatcgtc
     2461 gttggccaga tgggagaggt caacaagccg aaggacagcc tgctgacgtt ctatagcgcc
     2521 aagaccggca agaaaatcct caacctggag accggcctgt acgatatcgc cggcctggcc
     2581 tactcgccca agactggcct cttgtacgcc gtcgatttcg cctggatggc gccggaagaa
     2641 ggcggcctgt tccgccttga cgcggaaaac aaggacggtg ctcaaagcct taaggcggtg
     2701 aagatcgcca gcctcgacaa gccaacggcc ctggcgttcg ctccggacgg aacgctatat
     2761 gtgaccgtcg tcggcccgaa gggcgcagaa gaaaacgcac ccaaggaagg caaagtgctc
     2821 aagatcgcac cggggctgta gttcaacttg cctttagccg ggagcaaacc tgctcccgga
     2881 gaagtgcaac tggacgatcg acctgccaca caccgggacc agtttggtcc cggctaagaa
     2941 cactcaggaa tgcccggccg gcacggcttc gtgccgcgtg acggtgaagc cgagatcctc
     3001 gatcatcttg tagtcggctt cgggggcttg gcctttggtg gtgagatagt cgccgacgaa
     3061 catggagttg gcggcataga ggccgagggc ctggaggctg cccaggtgca actcgcggcc
     3121 gccggcgatg cggatctcgc tcgccggatt gacgaagcgg aacatggcca gggccttcag
     3181 acagtacggc ggagtgaggc gcgacatgcg ctgcaacggc gtgccgtcga tatcgatcag
     3241 gaaattgagc gggatcgatt cgacctccag cgcgcgcaat tccagggcca tccgcacgac
     3301 gtcctcatcg cgctcgccca tgccgataat cccgccgctg cagagctgca ggcccgcttg
     3361 ccgcactgcc ttgagcgtct cgacgcgatc gccgtaggtg tgcgtcgtgc agatctgctc
     3421 gtagaattcc gggctggtgt tcaggttgtg gttgactttg ttcacgccgc acgccgccag
     3481 ccggcccgct tgttcggagg tcaggaggcc gaggcaggca caaacttcaa ggtcgtactt
     3541 cgccttgatt tccggcacga tcgtttcgac cgcgtgcaac tcccgttcgc tcggcccgcg
     3601 ggcggaaatc acgatgcagt aggtcttgct cccgcgttcc gcagcgaccc gcgccccgtc
     3661 gagcagcttc tcgcggctga ggaggttgta ttgcgggatg tcggcggtcg aaaccttgga
     3721 ctgcgagcag tagtggcaat cctccgggca caggccgctc ttggcgttca tcaggaagta
     3781 gagctggacc gttttgccga agaaccgccg gcggacgcga aaggccgcgt cgaggatcgc
     3841 cagcagctca tcatccggcg cgcgaagcat cgccagggct tcgttcggcg taacgcggtg
     3901 gtcatcgagc acgcgctcgg cgaggcaagt ccagtc
//
LOCUS       DASUGB010000802         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1755597, whole genome shotgun sequence.
ACCESSION   DASUGB010000802 DASUGB010000000
VERSION     DASUGB010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755597"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3127)
                     /locus_tag="VFV87_15320"
     CDS             complement(<1..>3127)
                     /locus_tag="VFV87_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105189.1"
                     /translation="SFRILHGAVDKFRLSVPAGFEVTRVESPLLARWEEKADADGTKA
                     LEATLREPTSEQVVLQITANRSPSAAADWLASLQDWKFPQLTPFDTAGNVAVLGLLVE
                     DRLRPEKISPTNLLPIDSQAILGAIPASVLTAEPGAPTIRQVVSYYAPAGQYTLSASF
                     ARPPAGLKVAGNSLLVIADQGLTLQGGFALSPQAESLYGFAFTVPAGWQVTQVTAADG
                     TVLPIERYPANGGGTRISVRLPAGIGVGQTQTINYQAVSTPAGWLADWQTQKVTFPSL
                     LVENATEISGAIAAQALDDLIVRPEAPAGLVPLLDKEKDAYGLGSIPTALAYRYESGD
                     FSITLSLERTKPSLAAEVFSFFRLDRDNLIAHYELNYDVREARTRTLVFSLDKATPTE
                     ISIRGLDGVAVKEFTFNDEGTWRRWTVQLADRHVGRVRLAVDFQQQYDPQALTRSGLP
                     LIQAEEVEYQSAFFAVEGDPELEISVSTPARPVDPGELAGAEYVVGKRLLGAYGYVGN
                     RPTTSLQVARREAYTLPPALIQRAELVTKVSAASRRELKDGTAVGALAQSVARYDLLT
                     KATLLEIQLPPGSDLWSVLLDDHPTKPQIEGDSLLLSLPAQSALAIRKLQIVYESPSQ
                     PLSFSGTVAAIAPKLLIRAAGTDDEREIPQADLQWQLILPNGYQVRRAGGTVFTDEIP
                     QREMAAVKVAAVLYELAGGLNPFYSRSRGSGLSGGVTWTGAVAESKSVASHSSALLQD
                     DSRSMAYEGAAESTLSAPQTAAMPESMPAPAAEMPEDRPAETPPPAMPAEPPLAREPA
                     PQVPAPGVDVPKMDAGLAEDQAARRQGLGDLQGQLAIAQSKLWALEGVSSLKIDIQAD
                     SAPATTFVSLGESPRLEATVINRRRLDAAACGLASLVLLVGIGLTRRPARQKAAYVII
                     VLLASSVPLLLTSAFDEVGQVFDYVFYAGCLLIPYYLLAALVLRIFGWAKTKVPAGCC
                     ESAPAVQQIAAILVAVSILTLSASATAQEPADKLPEGVRITNLKDILPLLEGEQAPVA
                     VPADAVIVPYDP"
BASE COUNT          509 a          983 c         1097 g          538 t
ORIGIN      
        1 ggatcgtagg ggacaatgac cgcatcggcg ggaactgcaa cgggggcctg ctcgccttct
       61 aagagcggca ggatgtcctt gaggttcgtg atgcggacgc cttcgggaag cttatcggct
      121 ggttcttggg cggtggccga cgcgcttagc gtgaggatgc tgaccgcgac caggatcgca
      181 gcgatctgct gcacggcggg cgcggattcg cagcagccgg cggggacttt tgtcttggcc
      241 cagccgaaga tgcggagcac cagtgccgcg agcaggtaat atggaatcaa caggcagccg
      301 gcgtagaaga cgtagtcgaa cacctggccg acttcgtcga aggcgctggt gagcaagagc
      361 ggcacgctcg aagcgagcag cacgatgatc acgtaggccg ccttctgccg ggcgggccgc
      421 cgtgtgaggc cgatgccgac cagcaacacc agcgacgcca atccgcaggc cgccgcatcg
      481 agccgccggc ggttgatcac cgtcgcctcc agccgcggcg attcgcccag gctgacgaac
      541 gtggtcgccg gcgcggaatc cgcctggata tcgatcttga gcgagctgac cccttcgagg
      601 gcccagagct tgctttgtgc gatggcaagt tgcccctgca agtcgccgag gccttggcgg
      661 cgggctgcct ggtcttcggc cagccctgcg tccattttcg gtacgtctac gcccggcgca
      721 ggaacttgcg gtgctggctc cctagccagc ggtggctcgg ctggcatggc cggcggcggc
      781 gtttccgcag ggcgatcctc tggcatttct gctgccgggg caggcatgct ttcgggcatg
      841 gccgcggttt gcggagcgga gagtgtgctt tccgctgctc cttcgtaggc catcgacctc
      901 gaatcgtcct ggagaagtgc actggaatgg ctcgcgaccg acttcgactc agcaacggcg
      961 ccagtccaag tgaccccgcc agacaggcca gatccgcgcg accgcgagta gaacggattc
     1021 agcccccccg ccagctcata cagcaccgcc gcgaccttga ccgcagccat ctcgcgctgc
     1081 ggaatttcgt cggtgaacac ggtgccgccg gcgcggcgaa cctggtagcc attgggcagg
     1141 atgagctgcc actgcagatc ggcttgcggg atttcgcgct cgtcgtcggt gccggcagcg
     1201 cggatcagca gcttcggggc gatcgccgcg acggtgcccg agaagctcag cggctggctg
     1261 ggcgattcat agacgatctg cagcttccgg atcgccagcg cggattgcgc cggcaggctg
     1321 aggagcaggc tgtctccctc gatttgcggt ttcgtggggt gatcgtcgag caggacgctc
     1381 cacaggtcgg agccgggcgg aagctggatc tccaggagcg tggcttttgt gagcaggtcg
     1441 tagcgggcga ccgattgggc cagcgcgcca accgcggtgc cgtccttcag ctcacggcgc
     1501 gacgcggccg agaccttcgt caccagctcg gctcgctgaa tgagggccgg cggcagcgtg
     1561 taggcctcgc ggcgggccac ttgcagcgac gtggtcgggc gattgccgac atagccatac
     1621 gcccccagca gccgcttgcc gacgacgtac tccgccccgg cgagttcacc tggatcgacc
     1681 gggcgggccg gcgtcgaaac gctgatctcc agctccgggt ccccctcgac ggcaaaaaac
     1741 gccgactggt actcgacctc ttccgcctga atgagcggca ggccggatcg cgtcaatgcc
     1801 tgcggatcgt attgctgctg gaaatcgacc gccaggcgca cgcggccgac gtgccggtcg
     1861 gcaagctgca cggtccagcg tcgccaagtg ccttcgtcgt tgaacgtgaa ctcttttact
     1921 gccacgccat ccagcccgcg aatgctgatc tctgtcggcg tcgccttatc gagcgagaac
     1981 acgagcgtgc gcgtgcgggc ctcgcggacg tcgtagttga gctcgtagtg ggcgatcagg
     2041 ttgtcgcggt ccaggcggaa gaacgagaac acctcggctg cgagcgacgg cttcgtccgc
     2101 tccagggaca gcgttatcga aaaatcgccc gactcgtagc gataggcaag ggccgtgggg
     2161 atgctgccca ggccatacgc gtccttttcc ttgtcgagga gcggcaccag gccggcgggc
     2221 gcttcggggc gcacaatgag gtcgtcgagc gcctgggcgg cgattgctcc gctgatttcg
     2281 gtcgcatttt cgacgagcag gctcgggaac gtaactttct gcgtttgcca atcggccagc
     2341 cagccggcgg gcgtgctgac cgcctggtag ttgatcgtct gcgtctggcc gacaccgatt
     2401 cctgcgggga ggcggacgct gatccgcgtg ccgccgccgt tggccggata gcgctcgatc
     2461 ggcagcacgg tgccgtcggc cgcggtgacc tgggtcacct gccagccggc gggaacagtg
     2521 aacgcaaagc cgtagaggct ttcggcctgc ggactaagcg caaagccgcc ttggagcgtg
     2581 agcccctgat cggcgatcac caggagcgaa ttgccggcga ctttcaggcc ggccggcgga
     2641 cgggcgaagc tggcggatag cgtgtattgg ccggcaggcg cgtaatagct gacgacctgg
     2701 cggatcgtcg gcgcgccggg ctcggcggtg aggacgctgg cgggaatcgc ccccagaatc
     2761 gcctgcgaat cgatcggcag caggttggtt ggcgagattt tctccggccg caggcggtct
     2821 tcgacgagca ggcccaggac cgcgacattg ccggcggtat cgaacggagt cagttgcggg
     2881 aatttccagt cctgcaggct ggcgagccaa tcggcagcgg cggacggcga gcgattggcc
     2941 gtgatttgca gcacgacctg ctcgctggtc ggttcgcgga gcgtggcttc gagcgccttc
     3001 gtaccgtcgg catcggcttt ctcttcccag cgggcgagga gcggcgactc gacgcgcgtc
     3061 acttcgaagc cggccggcac gctgaggcgg aacttatcga ccgcaccgtg cagaatgcgg
     3121 aacgaga
//
LOCUS       DASUGB010000803         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1338253, whole genome shotgun sequence.
ACCESSION   DASUGB010000803 DASUGB010000000
VERSION     DASUGB010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338253"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VFV87_15325"
     CDS             complement(<1..1816)
                     /locus_tag="VFV87_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HEX5105190.1"
                     /translation="MSDPADQILDAQLRDVPLPDGLLARLKSSLVPSDEQLNAALVAV
                     AVPATVLARLREIPGDVAVDEAVNEVAAPPALIYALRRPTWGQRFARAGRSLARLSVA
                     ALWFVAISAALAGGLRLMIEGTYPPPKAELEIAVIYDGPLSVDAQMQRQREIGVPLAM
                     AVPSAAEEADSIPVAANAGDKTNPPIARIAQPEVDEPGAGGPVAQWVSLVSSGLRPMD
                     DAVLLRYGVLGSPHYADDHLPDLIAPRQPRAAGIQPPLVRGYDRAFFLKHRVFPPIAP
                     AANPKLADLDVPLRPGSDALARIESTLAEGRTPEPHDVRVEDLIAAMDYRLAPAPAGR
                     LALRTAAGPAIFGPPESGLLLVGVQAGGLAQRPQPVTHLVVALDLSHSMSRGGRLPIV
                     QKGLSRLLDQLSSRDRISLVVFNEEVVQVVEAASRDEADSIRRLLGELAPRGGTNLAA
                     GLQQAASLAMADAAGPAAARRLVLITDSQATMPDDTRSGIEQLLAAAGAVGVRLDVLD
                     VSQRAQVDATLQGWSLDLGGDLRPIADARQMTRSLLEVLSGREATIARDACLKLHFNP
                     KTVAAYRLVGHEANPLADLTPAAVDAEFAAGEAAAALVE"
     gene            complement(1925..>2360)
                     /locus_tag="VFV87_15330"
     CDS             complement(1925..>2360)
                     /locus_tag="VFV87_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5105191.1"
                     /translation="IAFRLTGDRHEAEDMVQEAFRSAWKSRELYQPGRGDRAWLASIL
                     RRRVIDRYRRQAPPSTFASDSPHEVEVHDTDPISHQYTDEMQHALAQLPEELRETLLL
                     VVVGELTHQEVADLMEIPLGTVLSRVSRARRRLRELLLAAKT"
BASE COUNT          373 a          778 c          833 g          376 t
ORIGIN      
        1 gttcgaccaa cgccgcggcc gcttcgccgg cggcaaactc ggcatcgacc gcggccggcg
       61 tcagatcggc gagcggattg gcctcgtgcc cgaccaggcg ataggcggcc accgtcttcg
      121 gattgaagtg cagcttgagg caggcatcgc gggcgatcgt cgcttcccgg ccgctgagga
      181 cctcgaggag cgagcgcgtc atctgccgcg catcggcgat cggccgcaaa tcgccgccca
      241 gatcgagcga ccagccctgc agcgttgcat cgacctgcgc tcgctgcgaa acgtcgagca
      301 cgtccagccg cacgcccacc gctccggcag ccgccaggag ctgctcgatg ccggatcgcg
      361 tgtcgtcagg catggtcgcc tggctgtcgg tgatgaggac caatcggcga gccgccgcgg
      421 gaccggcggc atcggccatt gccagcgaag ccgcttgctg caggccggcg gcgagattcg
      481 tgccgccgcg cggggccagc tcgccgagca gacggcgaat ggaatcggcc tcgtcacgcg
      541 aggcggcctc gacgacctgc acgacttctt cgttgaatac aaccagcgag attcgatcgc
      601 gcgagctgag ctgatcgagc aaccggctga ggcccttctg cacgatcggc agccgtccgc
      661 cgcggctcat gctgtgcgag aggtcgaggg ccacgaccag gtgcgtgacc ggctgcggac
      721 gctgagccag tccgccggcc tgcacgccga ccagcaagag accgctttcc ggcgggccga
      781 aaatcgccgg cccggcagcg gtccgcaaag cgagccgtcc cgcgggtgcg ggtgccaggc
      841 ggtagtccat tgctgcgatc aggtcttcga cgcgcacgtc gtgcggctcc ggcgtgcggc
      901 cttcggccag cgtgctttcg atccgcgcca gagcatcgct gccggggcgc agcggcacat
      961 cgagatcggc cagcttcgga ttcgcggcgg gcgcgattgg cggaaagacg cgatgtttga
     1021 ggaagaatgc ccggtcgtag ccccgcacca gcggcggctg gatgcctgcg gctcgcggct
     1081 ggcgcggagc gatcagatcg ggcaaatgat cgtcggcata gtgcggcgag ccgagcacgc
     1141 cataacgcag cagtaccgcg tcgtccatcg gccgcaggcc agacgagacg agcgaaaccc
     1201 actgagcgac ggggccgccg gcaccaggct cgtcaacttc cggttgggcg atgcgggcga
     1261 tgggcgggtt cgtcttgtcg ccggcattgg cggcgacggg tatggagtct gcttcttccg
     1321 ctgcggatgg aacggccatc gccaggggca cgcctatctc ccgctgccgc tgcatctgcg
     1381 cgtccactga gagcggaccg tcgtagatga cggcgatctc cagttccgct ttgggcggcg
     1441 gatacgtgcc ctcaatcatc agccgcaagc cgcccgccag cgccgccgag atggcgacga
     1501 accacagcgc cgcgaccgac aggcgcgcga gcgaacggcc agcgcgggcg aatcgctggc
     1561 cccatgtcgg ccggcgcagc gcatagatca gcgccggcgg ggccgcgact tcgtttaccg
     1621 cttcatcgac ggccacgtcg cccggaatct cccgcagccg cgcgagcacg gtcgccggaa
     1681 ccgccaccgc gaccagtgca gcattgagct gctcgtcgct gggaaccagc gacgacttca
     1741 gccgggcgag caggccgtca ggcagcggca cgtcgcgaag ctgggcatcg agaatttggt
     1801 cggcggggtc ggacacggta ggcgggggaa ttcagaattc agaatgcaga attgggactt
     1861 tgtttggttc ttgtctcttg tttcttggag ctttggattt cttggagctg cggagttttc
     1921 tccgctacgt tttcgcggcc agcagtaatt cccgcagtcg ccgcctggca cggctgacgc
     1981 gggagaggac ggttccgagg ggaatttcca tcaggtcggc gacctcttgg tgggtcagct
     2041 cgccgacgac gaccaagagc agcgtctcgc gcagctcctc gggaagctgg gccagggcgt
     2101 gctgcatctc gtcggtgtat tggtgggaga tcggatcggt gtcgtggacc tcaacctcgt
     2161 gcgggctgtc gctggcgaag gtgctcggcg gggcttggcg gcggtagcgg tcgatcaccc
     2221 gccgccggag gatcgaggcc agccaggccc ggtcgccccg ccccggctgg tagagctcgc
     2281 ggctcttcca cgccgagcgg aacgcttcct gcaccatgtc ctccgcttcg tggcggtcgc
     2341 cggtcaatcg aaaggcgatc
//
LOCUS       DASUGB010000804         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_922333, whole genome shotgun sequence.
ACCESSION   DASUGB010000804 DASUGB010000000
VERSION     DASUGB010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922333"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFV87_15335"
     CDS             <1..1486
                     /locus_tag="VFV87_15335"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5105192.1"
                     /translation="PSDFGHTGMAPTHPELLDYLANELTSGDWHLKRLHRMMMTSATY
                     RQSSRILDFGLPILDSTENTAAIQNPKSKIQNPQLVDPDNTLLWRQNFRRLEAEALRD
                     SILAASGELNLQMGGRGIFPTLPPEVLSKQSRPGAGWENNAPADQQARRSVYIFVKRT
                     LGVPLLESFDVATPDSPAASRNVTTVAPQALILLNSAFMQYQSAALAERVVLSAGADR
                     PQQIAAAYRLALGREPTDRELGLALAFLERETARWQAAAERGRESIADTSRPLENGAP
                     QLTPDPLASEEVQLLGWKHFGGQWRKREDGGCQVEAHPGAKVVRESLELTDGTVEAQV
                     MLLDGGGDAGLIVRVSEPQHGTNALTSYNINLRKDQLRLGKHENNWQALRTTNINIEP
                     EKWHDLKVELAGGRIRVWLGGSKEPQIDYTDPEPLKAGSVGFRTFQVGAAVRQIRIIR
                     GDKSEDVPMEYVEATDATPSSAQARALAELCKLFLNLNEFVYID"
     gene            1583..>2288
                     /locus_tag="VFV87_15340"
     CDS             1583..>2288
                     /locus_tag="VFV87_15340"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105193.1"
                     /translation="MTNPRSPCCTGHRREFLWQMGAGFAGLALTSLLDRDGFFARHAF
                     SAEASGGRQPPEASLANPLAPKPAHFPAKAKHVIFLFMYGGPSSMDTWDYKPELQKRD
                     GETIDIEIRRGSIQKQKLLASRRRFQQYGQAGLWGADCFPHINQHLDKLCLIKSLYAD
                     TFAHGSAMIQLNSGRIIQGYPSIGSWLNYGLGSQNENLPGYVVMLDPRGGPISGPANW
                     SSGFMPAAYQGTHLRHK"
BASE COUNT          456 a          797 c          669 g          366 t
ORIGIN      
        1 ccccagcgat ttcggccaca cgggaatggc acccacgcat ccggaactgc tcgactatct
       61 ggccaacgag ctgaccagcg gcgactggca cctcaaacgc ctgcaccgaa tgatgatgac
      121 ctcggcaacg taccggcagt catcgagaat tttggatttt ggattgccga ttttggattc
      181 gacggaaaac accgctgcaa tccaaaatcc aaaatccaaa atccaaaatc cccagctcgt
      241 cgatcccgac aacacgctcc tctggcggca gaacttccgc cgcctggaag ccgaggcgct
      301 gcgcgacagc atcctcgccg ccagcggcga gctgaatctg cagatgggtg gccgcggcat
      361 tttcccgacg ctgccgccgg aggttctctc gaaacaatcg cggcccggcg cgggttggga
      421 aaataatgct cccgccgatc agcaggcccg ccgcagcgtc tatatcttcg tcaagcggac
      481 gctcggcgtg ccgctcctcg aatcgttcga cgtcgccacg cccgacagcc ccgccgccag
      541 ccgcaatgtg acgaccgtcg ccccgcaagc gctcatcctg cttaacagtg cgtttatgca
      601 gtaccaatcg gccgcgttgg ccgagcgcgt tgtgctatcg gcaggcgcgg accgccctca
      661 gcaaattgcc gccgcctatc gcctcgctct cggccgcgag ccgaccgatc gcgaactcgg
      721 cctggccctc gccttcctcg aacgcgaaac cgcacgctgg caagccgccg ctgaaagggg
      781 tcgggagtca attgcggata cctctcgtcc attggaaaac ggggcgccgc aattgactcc
      841 cgaccccctc gcgtccgaag aggtgcaact cctcggctgg aagcacttcg gcggccagtg
      901 gcggaagcgc gaggatggcg gctgccaggt ggaagcccat ccgggggcga aagtcgtccg
      961 cgagagtctg gagctgaccg acggcacggt cgaggcgcag gtcatgctgc tggatggcgg
     1021 cggcgatgcc ggcctgatcg tccgcgttag cgagccgcag catggtacga acgccttgac
     1081 ctcttacaac atcaacctcc gcaaagacca gctccgcctc ggcaagcatg agaacaattg
     1141 gcaggccctg cggacgacga acatcaacat cgagcccgag aagtggcatg accttaaagt
     1201 cgagctcgcc ggcggccgca ttcgcgtctg gttgggcggc tcgaaagagc cgcagatcga
     1261 ctacaccgac cccgagccgc tgaaagccgg cagcgtcggc ttccgcacgt tccaggtcgg
     1321 tgccgccgtg cgacagattc gcatcatcag gggcgacaaa tcggaagacg tgccgatgga
     1381 atacgtcgag gccacggacg cgacgccgtc cagcgctcaa gcccgcgccc tggccgagct
     1441 ctgcaagctg ttcctgaacc tcaacgaatt tgtctatatc gactgacctg ttcctagccg
     1501 ggagcaaacg tgctcccgga gaactgccaa ttgacaatcc actcgcactg ctccgggacc
     1561 aggtttggtc ccggctaaaa gcatgaccaa tccccgctcg ccctgctgca ccggccatcg
     1621 ccgcgaattc ctctggcaaa tgggcgccgg cttcgccggc ctcgccctca cgtcgctcct
     1681 cgaccgggac ggcttcttcg cgcgccacgc cttcagcgcg gaggcgagcg gggggcgtca
     1741 gccccctgag gcatcgctcg ccaacccgct cgcccccaag cccgcccatt ttcccgccaa
     1801 agccaaacac gtcatcttcc tgttcatgta cggcggcccg tcctcgatgg acacctggga
     1861 ctacaagccc gagctgcaaa aacgcgacgg cgaaacgatc gacatcgaaa tccgccgcgg
     1921 cagcatccag aagcagaagc tgctcgccag ccggcgcagg ttccagcaat acggccaggc
     1981 cggcctgtgg ggggccgatt gcttcccgca catcaatcag cacctcgaca aactctgcct
     2041 catcaagagc ctgtatgccg acacgttcgc ccacggttcg gcgatgatcc agctcaacag
     2101 cggacggatc atccagggct atccgtcgat cggctcgtgg ctgaactacg gcctgggaag
     2161 ccagaatgag aacctgccgg gctacgtcgt gatgctcgac ccccgcggcg gcccgatcag
     2221 cgggccggcc aactggtcca gcggcttcat gccggcggcg tatcagggga cgcacctgcg
     2281 gcacaagg
//
LOCUS       DASUGB010000805         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2034197, whole genome shotgun sequence.
ACCESSION   DASUGB010000805 DASUGB010000000
VERSION     DASUGB010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034197"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2407
                     /locus_tag="VFV87_15345"
     CDS             <1..2407
                     /locus_tag="VFV87_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5105194.1"
                     /translation="LIDKQGNFGSIAGMPPAAMRYTEARLSPIAALMLAESDLDTVDF
                     VPNYDESRVEPSVLPSKFPNLLVNGAQGIAVGMATSLPPHNLREICSALIRVLDEPDV
                     SLDELLEIVPGPDFPTGGQICGTAGLRRGYMTGRGTITIRARAKIEEHKKDRYRIVVR
                     EIPYQATRDGVLEKLNALMDDERIKGISEVTDYSDLQHPVHIVIDVKRDADPEVVLNQ
                     LYQFSPLQDSFSIILLALVDGKPRTCSLKQLLEEYLRHRVTVIRRRTQFLLGKARKRK
                     HTVEGLLLALANIDEIIRLIRASKTQAEAKVALMGVECPASMMERALGDAGFKVFQEE
                     RGAADVYRLTAVQAEAILRMTLGQLVNLEQERLAGEHAELLEEIGEYLRILSDEANIR
                     AIIRDDLLEIQKKYGDDRRTEITGEEIGSVDLEDLITEETMVVSISHKGYIKRTPASV
                     YRAQRRGGKGLKGAETEEEDPIEHLFVASTHAYLLFFTNLGKVYWQKVYDLPELKRDS
                     RGRAIVNLLNLAEGEKIAECLAIRDFEAQGHYLMMATRKGLVKKTPLEDYSRPKKGGI
                     IAIKLREDDEVVDVVVTKPGDEVVLSSANGMAIRFSEADARPMGRNTSGVKGIGLVGD
                     DYVVGMVVADPEATLLTVCEKGYGKRTNFGPNEEAGDREQGTGDGEQETEPEDNLGPG
                     TLDVGPAAEAPEENADDEGPSGQRYRTQRRGGKGLRDIKTTERNGRVIGIVRIDDRDE
                     VLMMTARGKLQRIAAADLSVIGRNTQGVRIMSLDEGDTLVAVTRVPPEEEAAEPAATP
                     GIS"
     gene            2462..>2645
                     /locus_tag="VFV87_15350"
     CDS             2462..>2645
                     /locus_tag="VFV87_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HEX5105195.1"
                     /translation="MTLDNKRASLVAAIERLGSCAVAFSGGVDSAVVAQAAALAMGDK
                     AIAVTGVSASLASGELE"
BASE COUNT          558 a          806 c          857 g          424 t
ORIGIN      
        1 gctgatcgac aagcagggaa acttcggatc gatcgcgggc atgccgccgg ctgccatgcg
       61 ttataccgag gcccgcctgt caccgattgc ggctctgatg ttggccgaat cggacctcga
      121 cacggtcgat ttcgtcccca actacgacga atcgcgggtc gagccctccg ttttgcccag
      181 caagttcccg aacttgctgg tcaacggcgc ccagggcatt gctgtcggca tggcgacgag
      241 cctcccgccc cacaacctgc gcgaaatctg cagcgccctg atccgcgtcc tcgacgagcc
      301 ggacgtttcg ctggacgagc ttttggaaat cgtccccggc cccgattttc ccacgggcgg
      361 ccagatctgc ggcacggcgg ggctccgccg gggctatatg acgggccgcg gcacaatcac
      421 gatccgagcc cgggccaaga tcgaagagca caaaaaggac cgctatcgga tcgtcgtccg
      481 agaaatcccc tatcaggcca cccgcgacgg cgtgctcgaa aagctcaacg ccctgatgga
      541 cgacgagcga atcaagggca tctcggaagt caccgactac agcgacttgc agcacccggt
      601 acacatcgtg atcgacgtca aacgcgacgc cgatccggaa gtcgtcctca accagctcta
      661 tcaattctcg ccgctgcagg attcgttctc gatcatcctg ttggccctcg tcgatggcaa
      721 gccgcggacc tgctcgctca agcaactcct ggaggaatac ctgcggcacc gagtgacggt
      781 gatccgccgc cgcacgcagt tcctcctggg caaggcccgc aagcggaagc acacggtcga
      841 ggggctcttg ctcgccctgg cgaatatcga cgagatcatc cgcctgatcc gcgccagcaa
      901 gacgcaggcg gaggcgaagg tcgccctgat gggggtcgag tgcccggcca gcatgatgga
      961 gcgggccctg ggcgacgcgg gattcaaggt ctttcaggaa gagcgcggcg cggccgacgt
     1021 ttatcgcctg accgccgtgc aagccgaggc gatcctgcgg atgacactcg gccagctcgt
     1081 caacctggag caggaaaggc tcgccggcga gcatgcagag ctactcgaag aaatcggcga
     1141 gtacctgcga atcctttccg acgaggcaaa tatccgagca atcattcgcg acgacctctt
     1201 ggagatccag aagaaatatg gcgacgaccg ccgcaccgag attaccggcg aagagattgg
     1261 cagcgtcgat ctggaggacc tgatcaccga agagacgatg gtcgtctcga tcagccacaa
     1321 gggctacatc aagcggacgc cggccagcgt ctatcgcgcc cagcgccgcg gcggcaaagg
     1381 cctgaaaggg gccgagaccg aggaagaaga cccgatcgag cacctgttcg tcgccagcac
     1441 gcacgcgtac ctgctgtttt tcacgaatct gggcaaggtc tattggcaga aggtctatga
     1501 cctgccggag ctcaagcgcg acagtcgcgg ccgggcaatc gtgaacctgc tcaacctggc
     1561 cgagggcgaa aagatcgccg aatgtctggc gatccgcgat ttcgaggctc aggggcacta
     1621 cctgatgatg gccacccgca agggcctggt gaaaaagacg ccgttggagg actacagtcg
     1681 gccgaagaag ggcggcatca tcgcgatcaa gctccgcgag gacgacgaag tcgtcgatgt
     1741 ggttgtcacc aagcccggcg acgaagtcgt cctctcctcg gccaatggca tggccattcg
     1801 cttcagcgaa gcggatgccc ggccgatggg gcgcaatact tcgggcgtca aaggcattgg
     1861 cctggttggc gacgactacg tcgtcggcat ggtggtcgcc gatcccgagg cgacgttgct
     1921 gaccgtgtgc gaaaaaggtt atggcaagcg gacgaatttc gggccgaatg aggaggcagg
     1981 ggacagggaa caggggacag gggacgggga acaggagaca gagcccgaag acaaccttgg
     2041 acctgggact ttggatgttg gacctgccgc cgaagcgccc gaggaaaatg ccgacgacga
     2101 aggtccttcg ggccagcgtt accgcacgca gcgccgcggc gggaagggcc tgcgcgacat
     2161 caagacgacc gagcgcaatg gccgcgtgat cggcattgtc cgcatcgatg accgcgacga
     2221 ggtgttgatg atgaccgccc gcggcaagct ccagcggatc gccgccgctg atctcagcgt
     2281 catcggccgt aacacacagg gcgtccgcat catgagcttg gacgaaggcg acaccctcgt
     2341 cgccgtgacc cgcgtcccgc ccgaagaaga agctgccgaa cctgctgcga cgcctggaat
     2401 ctcgtagcag caaggcaaga tccgaattct gcacgatgca ctggcggcgt aagatagtga
     2461 catgacgctg gacaacaaac gtgcgtcgct ggtagctgcc atcgagcggc tgggaagttg
     2521 cgcggtggcg ttttctggcg gtgtcgatag cgccgtcgtg gctcaagcgg cggccttggc
     2581 gatgggagat aaggcgattg cggtcaccgg tgtcagtgcc agcctggcca gcggtgagtt
     2641 agagg
//
LOCUS       DASUGB010000806         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1894463, whole genome shotgun sequence.
ACCESSION   DASUGB010000806 DASUGB010000000
VERSION     DASUGB010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894463"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFV87_15355"
     CDS             <1..207
                     /locus_tag="VFV87_15355"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912808.1"
                     /note="E3 component of pyruvate and 2-oxoglutarate
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5105196.1"
                     /translation="VGIVGPGAGELIAEAVLAIEMGCEVTDITESVHAHPTLSETLMN
                     AGETYFGTATEIYKPKRKPRAEAG"
     gene            complement(396..773)
                     /locus_tag="VFV87_15360"
     CDS             complement(396..773)
                     /locus_tag="VFV87_15360"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX5105197.1"
                     /translation="MPTPIAIAVVEHEGRFLIGQRPAGVPLAGLWEFPGGKIQPGETP
                     EAAAVRECLEETGLAVQPLFRYPDHEQHYDHGSVQLHFIACLPAASALPNPRPPFRWV
                     NRQHLAEYHFPAGNRGILELLHR"
     gene            complement(867..1688)
                     /locus_tag="VFV87_15365"
     CDS             complement(867..1688)
                     /locus_tag="VFV87_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX5105198.1"
                     /translation="MGMFDGKKGLILGVANDRSIAWAIAQVILEQGGLCGFSHLPDKP
                     DDERKKNRLRVAKCVEPFGPAAKFLMPLDVQKDEDIAAIIAQAKSEFGQIDFLLHSIA
                     FATLDDLKRDTIETSREGFKLAMEISAYSLLAVCNAAKEIMSPRSSILTLTYFGGEKI
                     VSGYNVMGICKAALDACMRYAAFDLGPRGIRVNALSAGPLKTLAGSAAGVGPMQELYE
                     KMAPLGRNITHEEVGKIGAFLLSDMSDGITAEIMHVDGGYNAMGSPGRLLDAYKS"
     gene            1856..3802
                     /locus_tag="VFV87_15370"
     CDS             1856..3802
                     /locus_tag="VFV87_15370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX5105199.1"
                     /translation="MSRSITPDLANLESHPALAETASRLFYWAASESDPAAYMRQALP
                     VVVQNLGGEYLAVVHGEKGQWRTLGASGPSRPLPTEQLADALDRDEPVVRGDWYVAPL
                     VPRGGTGELIAAYRIWNTAVERGGTLETLAEWLAAGLAQVRSRQRDRQQIQRLQTILA
                     IAAQWQQTQEMGELLARMAEAATKLLAAERASIFLWDKSSRTLVGRPALGVEGGELRI
                     ADDAGVVGQVVHSGQPRRVDADIAADQREIDRRIDQKLKFQTRSLVCVPLRGRKGDLF
                     GAFEVLNKIGGNFTPADEQALTELAAHAAVALENTQEWEQLLASRKQVADSAAEGVQL
                     IGQCPPIEALRSTIRRVADTELAVLVLGENGTGKEVVAQMVHYQSRRRHEPLVAVNCA
                     ALTETLLESELFGHEKGAFTDAREMRRGKFELAASGTLFLDEIGDMSLGGQAKLLRVL
                     EEKVVVRVGGSTPIPTNTRVIAATNQNLAELVRSRKFREDLYFRLNVVTIDMPPLRER
                     GDDVVLLAEHFLKQFSTKARRKTPKFTAAARKRLVAHSWPGNVRELRNLMERLAYLAP
                     DEQDKIDAPDLAFIMSPEESGAGPLSLDAPLAEATQQFQIEYIQRHIARSRGNMTAAA
                     EKLGLHRSNLYRKMRQLGMEAVEG"
BASE COUNT          711 a         1264 c         1352 g          620 t
ORIGIN      
        1 gtcggcatcg tcggccccgg cgcgggcgaa ttgatcgccg aagcggtcct ggccatcgag
       61 atgggttgcg aagtaaccga catcaccgaa tcggtccacg cccatcccac gctcagcgag
      121 acgctgatga acgccggcga aacatacttc ggcacggcca ccgagatcta caaacccaag
      181 cgcaaacccc gcgccgaggc gggctgatgt gtgggggccc gagctacggc tcagagcgcg
      241 ccagcgcgcg ggcttgaccc agggtctggc ctttaacggt cgctgtgttt ttgccggcct
      301 cttcaagaac ggtccgactt cgcaatccaa cgcgacgacg gagaatcaac tgccgccaat
      361 atcaacgaac gacgagaatt cggacgatcc accaactaac gatggagcag ctccagtatg
      421 ccgcgattgc ctgcgggaaa atggtactct gcgaggtgtt gccggttcac ccagcggaag
      481 ggcggacggg gattggggag tgcagatgca gccggcaggc aggcgatgaa gtggagctgc
      541 accgagccgt ggtcgtagtg ctgctcgtgg tcgggatagc gaaacagcgg ctgaacggcg
      601 aggcccgttt cttccaggca ttcgcggacg gcggcggctt ccggggtttc gccgggctgg
      661 atcttgccgc cggggaattc ccacagacct gccagcggca cgccggccgg gcgctggccg
      721 atcaggaagc ggccttcatg ctcgacgacg gcgatggcga tgggcgtggg catgggtagg
      781 gtgggacaag cccgccgcgg gcgctggccc accggaattg cgacacgagt ggtgggcctg
      841 cgctcgcgct gctcgctggt ccccccctac gatttatacg cgtccagcaa tcggccggga
      901 gagcccatcg cgttgtagcc gccatcgacg tgcatgattt cggcggtgat gccgtcggac
      961 atgtccgaga gcagaaacgc accgatcttg ccgacctctt cgtgcgtgat gttgcggcca
     1021 agcggggcca ttttttcgta cagctcctgc atcgggccga cgcccgccgc cgagccggcc
     1081 agtgtcttca gcggcccggc actcagggcg ttcacgcgga tgccgcgcgg gcccaggtcg
     1141 aacgccgcgt accgcatgca ggcgtcgagc gctgccttgc aaatgcccat cacgttgtag
     1201 cccgagacga ttttctcgcc gccgaagtag gtcagcgtca ggatcgagct ccgcggactc
     1261 atgatctcct tggccgcgtt gcataccgcc agcaggctgt aggcgctgat ctccatcgcc
     1321 agcttgaagc cctcgcggct ggtttcgatg gtgtcgcgtt tgagatcgtc gagcgtggcg
     1381 aaggcgatcg agtgcaagag gaagtcgatc tggccgaatt cgctcttggc ctgggcgatg
     1441 atcgccgcga tgtcctcgtc cttttgcacg tcgagcggca tcaagaactt cgccgcgggg
     1501 ccgaagggct cgacgcactt ggcgacccgc aggcggttct tctttcgctc gtcgtccggc
     1561 ttgtcgggca aatgcgaaaa gccgcacagc ccgccctgct ccaggatgac ctgggcgatg
     1621 gcccaggcga tcgagcggtc gttggccacg ccgagaatca gtcccttttt gccgtcgaac
     1681 atgcccatcg tgtgctcctc atgccgcgtt aaggaataaa gagaagataa caattgaccc
     1741 ggcaggcagc catcgcgggg cggggcggaa tcgaactgac gacgacaggg cgctggcgat
     1801 tcgataacat ggacgaataa ctatttcaac aaatcgacga cacacgacca ttcttatgtc
     1861 gcgttccatc acacccgatc tggcgaatct cgaatcgcac cccgccttgg ccgaaacggc
     1921 ttcacggctg ttctattggg cggcgagcga gtccgatccg gcggcgtaca tgcggcaggc
     1981 gctgccggtg gtggtgcaga acctgggggg tgagtacctg gcggtcgtcc acggcgaaaa
     2041 agggcagtgg cggacgctcg gcgcatcggg acccagccgc ccgctgccga cggagcagct
     2101 tgccgacgcc ctcgaccgcg atgagccggt ggtccgcggc gactggtacg tcgcaccgct
     2161 ggtgccgcgc ggcggaacgg gcgagctcat cgcggcttac cgcatctgga acacggcagt
     2221 cgagcgcggc ggcacgctgg agacgctggc cgagtggctg gccgcgggcc tcgcgcaagt
     2281 ccgcagccgg cagcgcgacc gccagcagat ccagcggctg caaacgatcc tggcgatcgc
     2341 cgcccagtgg caacagacgc aagagatggg tgagctgctc gcgcggatgg ccgaggcggc
     2401 gacgaagctg ctcgcagccg agcgggccag catcttcctg tgggacaaga gcagccgcac
     2461 gctcgtcggc cggccggcct tgggtgtcga agggggggag ctgcggattg cggacgacgc
     2521 gggcgtcgtc ggccaggtcg ttcacagcgg ccagccgcgg cgcgtcgatg ccgacatcgc
     2581 cgccgaccag cgcgagatcg accgccgcat cgaccagaag ctgaagtttc agacgcggtc
     2641 gctggtgtgc gtgcccttgc gagggcgcaa gggggacctg ttcggcgcgt ttgaggtgct
     2701 caacaagatc ggcggcaact tcacgccggc cgacgagcag gcgctcacgg aactggccgc
     2761 gcacgccgcc gtcgccctcg aaaacacgca ggaatgggag cagcttctcg cctcgcgcaa
     2821 gcaggtcgcc gattcggctg ccgagggcgt gcagctcatc ggccaatgcc cgccgatcga
     2881 ggcgctgcgc tcgacgatcc gccgcgtggc cgatacggag ctggccgtgc tggtgctcgg
     2941 cgagaacggc accggcaagg aagtcgtcgc ccagatggtc cactaccaaa gccgccgccg
     3001 ccacgagccg ctagttgccg tgaactgtgc ggcgctgacg gagacgctgt tggagagcga
     3061 gctgtttggc cacgagaaag gggcgttcac cgatgcccgc gagatgcgcc gcggaaaatt
     3121 cgagctggcc gccagcggca cgctgtttct cgacgaaatc ggcgacatga gcctgggagg
     3181 ccaggcgaag ctgctccgcg tgctggagga gaaggtcgtt gtccgcgtcg gcggctccac
     3241 gccgattccg accaacaccc gcgtgatcgc cgcgaccaac cagaacctgg ccgagctggt
     3301 ccgcagccgc aagttccgcg aggacctgta cttccgcctg aacgtcgtca cgatcgacat
     3361 gccgcccttg cgcgagcggg gcgacgacgt cgtgctgctc gccgagcact tcctcaagca
     3421 attttcgaca aaggcccgcc ggaagacacc caaattcacc gcggcggcgc ggaagcgcct
     3481 ggtggcccat tcgtggccgg ggaatgtccg cgagctgcgg aacctgatgg agcggctggc
     3541 gtatctcgcg cccgacgagc aggacaagat cgacgcgccg gacctggcat tcatcatgtc
     3601 gcccgaggaa tcgggcgcgg gaccgctatc gctcgacgcc ccgctggccg aggccacgca
     3661 gcaattccag atcgagtaca tccagcggca catcgcccgc tcccgcggca acatgaccgc
     3721 cgccgccgaa aagctcggcc tgcaccgcag caatctctac cgcaagatgc gccagctcgg
     3781 catggaagcg gtggaaggat gagcgagaca atcgttagtg gcgccggagg gcggcattcc
     3841 aggcagtgcg gaggcgctgg caacgctcgc gggtccacca gcggaggaat tgttggcggc
     3901 gggcggcgag gtggcgggtc tcgcgcccgg tcgcggcctt caaagcg
//
LOCUS       DASUGB010000807         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_783978, whole genome shotgun sequence.
ACCESSION   DASUGB010000807 DASUGB010000000
VERSION     DASUGB010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783978"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFV87_15375"
     CDS             <1..281
                     /locus_tag="VFV87_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105200.1"
                     /translation="AWRRKRQGLPEDESSIASRHDGPAEIVEHDERLARLRTALQELR
                     PEEQEVFLLRQNGDLTYEEIAEMLGVPTGTVKTRMRLALTRLREVLAE"
     gene            377..>2108
                     /locus_tag="VFV87_15380"
     CDS             377..>2108
                     /locus_tag="VFV87_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105201.1"
                     /translation="MTSDINMGQLREKMWDLVYGLLEPAESQALISRIKSDPQAARLY
                     AEVRLQADLVGYAAQVEDSSLVLSADHAARETAPAAEKSVAAKTAAAGKPAPASLARS
                     RSAGGWLAGLVAAALLLLIGVGLMWPQMGPQTTAFVVIDIEAPRSLPGGLPNKVPVKI
                     TNLEQVGQPAEGEVAVLAADGSEQFRDRFDTDDLGWAEVEIPGHAMQRGAKLRVEAVR
                     KKLAANAMARGERDEHLAEQAGGEAVPRSLEERESLVSTLTAELPVREEPKLQYFLVE
                     NPVPEAGQTNRFALWNLRPFSATPAPAQVSGAAIESLDRKALAEPMWAADADGVVVGE
                     IPAAQTEHLGDRLLALKQADGTTYYTPIDSLAKRYYDEATRQNRHRAAGLDQLALLRD
                     KEALREQKAESKDATSARESIPAGAAGSGEADAKGSLASGSVSGKKVGKELAKSLGQQ
                     NQQDQPEAAERSLALAFENQARGENHAQVQIPAKYADKRLLVRASIWDEQIAEKEIRP
                     EDLVQREGAVERSDVAKSENETEELASRSYGFILPKDVSGPVKLQLFDLSSSTPELVD
                     EQTFVRQSSRK"
BASE COUNT          427 a          627 c          733 g          321 t
ORIGIN      
        1 cggcctggcg tcgaaagcgc cagggcctcc cggaggacga atcgagcatc gccagccggc
       61 acgatgggcc ggctgagatt gtggaacacg acgaacggtt ggcccgcctg cggacggctt
      121 tgcaggagct tcgtccggag gagcaggaag tattcctgct gcggcagaac ggcgacctca
      181 cgtacgagga aatcgccgag atgctcggcg tgccgacagg gacggtgaaa acgcggatgc
      241 ggctggcact aacgcggctg cgcgaggtgc tggcggagta agcgatacgg tcattggtca
      301 cttgtcattt gtcatttgtt caatggcgtg ggcaagcgcc gaacaaaaat cacaaatgac
      361 gaaggaccga tgaccattga caagtgacat aaacatgggc cagcttcgcg aaaaaatgtg
      421 ggacctggtg tacgggctgc tcgagcccgc ggagagccag gccctgatct cgcgcatcaa
      481 gtccgatccg caggcggctc ggctgtatgc ggaagtgcgc ctgcaggccg atctggtcgg
      541 ctatgcggcg caggtcgaag attcgtcgct cgttctgtcg gccgatcatg cggcccgcga
      601 aacggcgccc gccgcggaga agtctgtcgc ggctaagaca gccgcagcgg ggaagcccgc
      661 gccggcatcg cttgccaggt cgcgttccgc cggcggttgg ctggcgggac ttgtcgctgc
      721 cgcattgctc ctactcattg gcgtcggcct gatgtggccg caaatggggc cgcagacaac
      781 ggcgttcgtc gtgatcgaca ttgaagcccc gcggtctctc ccgggcggct tgccgaacaa
      841 ggtgccggtg aagattacga acctggagca agtcggccag ccggccgagg gcgaagtcgc
      901 cgtgctcgcc gcggatggca gcgagcagtt ccgcgaccgg ttcgacacgg acgatctggg
      961 ctgggccgaa gtcgaaatcc ccggccatgc catgcagcgg ggcgcgaagc tgcgggtcga
     1021 ggcggtccgg aagaagctcg cggccaatgc aatggctcgc ggagagcggg atgaacatct
     1081 cgcggagcag gcgggaggag aagccgtccc ccgctcgctg gaagagcgcg aatcgctcgt
     1141 ctcgacgctg actgcagaac tgccggtccg cgaagagccg aagctgcagt acttcctggt
     1201 cgagaatcct gtccccgaag caggccagac caatcgcttc gccttgtgga acctgcggcc
     1261 cttcagcgcg acccccgcgc cggcccaggt ttccggggcg gccatcgagt cgctcgatcg
     1321 caaggcgctc gccgagccga tgtgggcagc cgacgcggac ggcgtcgtgg tcggcgagat
     1381 tccggccgcc caaacggaac acctgggcga ccggctgttg gccctcaagc aggctgacgg
     1441 cacgacctat tacacgccga tcgacagcct cgccaagcgg tattacgacg aggccacgcg
     1501 ccagaaccgc cacagggcgg ctggattgga ccagttggct ctgttgcgcg acaaagaggc
     1561 gctacgggag caaaaggctg aaagcaagga cgcgacgagc gcacgggagt cgattccggc
     1621 cggcgccgct ggttcgggag aagcggacgc caagggctcg ttggcgagcg gcagtgtgag
     1681 cggcaagaaa gtcggcaagg agctggcgaa atcgctcggg cagcagaatc agcaggatca
     1741 accggaagcg gccgagcggt ccctcgcgct ggcctttgaa aatcaggccc gaggtgagaa
     1801 tcacgcacag gtgcaaattc cggcaaagta tgccgacaag cggctgctgg tccgggccag
     1861 catttgggac gagcaaattg ccgagaagga aatcaggcca gaggacctgg tacaacggga
     1921 aggcgcagta gagcggtccg atgtcgcaaa aagcgagaac gaaaccgagg agcttgccag
     1981 ccgctcgtac gggttcattt tgcccaagga tgtcagcggg cctgtcaagc tccagttgtt
     2041 cgacctctcc tcctcgacgc cggagcttgt cgatgagcag acgttcgtcc gccagtcgtc
     2101 gcgcaagc
//
LOCUS       DASUGB010000808         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_644078, whole genome shotgun sequence.
ACCESSION   DASUGB010000808 DASUGB010000000
VERSION     DASUGB010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644078"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..495
                     /locus_tag="VFV87_15385"
     CDS             229..495
                     /locus_tag="VFV87_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105202.1"
                     /translation="MAALAQGLVAAAVVLLVRALRVAVALLAVTLWPRVRLLLVAALC
                     LLAAALLLVRLLALPQAEAVAPVVEVEVAQRTRLSSAAMAGASP"
     gene            492..803
                     /locus_tag="VFV87_15390"
     CDS             492..803
                     /locus_tag="VFV87_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105203.1"
                     /translation="MTGATNVRAGTEIKAEAAASPVSISLSELESGSWVIFELPGFTT
                     ASAGTQQDSLESLRKASGTSYYKGDSSLWVKVVSTGNSGNPPGGRGGRGGAATTLQVS
                     K"
     gene            complement(855..2189)
                     /locus_tag="VFV87_15395"
     CDS             complement(855..2189)
                     /locus_tag="VFV87_15395"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX5105204.1"
                     /translation="MEAVIWGMPAVNYERMLQAAIDNGAKLNQVVYWSRPVNSRTRTL
                     TPNPDTIYLNPFYDTSTGPVVVEIPPATADHVIVGSFDVSWQNALMDVGPAGEDKGKG
                     AKYLILPPGYEGEPPEGYIAMRSETCRGFVVLRSNFKSRSDADIKSAVDHGKKVKVYP
                     FGGDAGSTVFVDVYDKPFNATIPYDATFFEYLNRFVQAEPWLTRDKVMIDSLKTIGIE
                     KGKPFEPDAKTRAILEKAVKEAHAELALKYELGFATPFFEGTRWGLPIPKETVAGLGN
                     GFADPNQYGIDGRGVVYHFAYFSPKTFGGGQFYLLNLSDRSGQPLEGNKTYRLTIPPN
                     APMEQYWSVTAYDRATHALIAGMSRPSLASSDTTLQKNADGSTDIYFGPEAPAGKEPN
                     WVPTDPKGQFELLFRLYGPKKEFFEMKWKLPDVEEDSTAARRSSNHWQARAS"
BASE COUNT          411 a          788 c          896 g          510 t
ORIGIN      
        1 gccgatggtc gttcagcggt gaattcaata gttttggagg ctggaatggc gcggtgttca
       61 aggacatcga cggctcgctc ggcggcggcc ccaattccta catcgtcatc gataacggga
      121 tcgcggccgc ggccgatgac acagactgcc gaatcgagcc gacctggggt gccgcagtgt
      181 gcaagggtga tttgggtcgc ctcacgataa acgccggcgg cggcggtcgt ggcggccctg
      241 gcgcagggcc tggtcgcggc cgcggtggtg ctcctggtgc gggcgctccg cgtggcggtg
      301 gcgctcctgg cggtaacgct ctggcccagg gtccgcctgc tgctggtggc ggcgctgtgc
      361 ctgctggcgg cggcgctcct gctggtcagg ctcctggcgc tgccgcaggc cgaggcggtc
      421 gcgccggtgg tggaggtcga ggtggcgcag cgaacccggt tgtcctcagc cgcaatggca
      481 ggagcttcac cgtgaccggg gccacgaacg tacgcgcggg caccgagatc aaggcggaag
      541 ccgcagcgtc gcccgtgtcc atcagcctga gcgagctgga gagcggctca tgggtgattt
      601 tcgagctgcc gggattcacc accgcaagcg cgggaacgca gcaggacagc ctggaatcgc
      661 tgcgcaaggc cagcggcacc tcgtactaca aaggcgacag ctcgctgtgg gtcaaggttg
      721 tctccaccgg taattccggt aatcctcctg gtggccgcgg cggtcgtggc ggcgctgcaa
      781 cgaccctcca ggtcagcaag taagctgccc tcgctataga tgcccccggc gcaagccggg
      841 ggctcgatgc gccttcagct ggcccgagcc tgccagtggt tcgatgatcg cctggctgcg
      901 gtggagtcct cctcgacatc cggcagcttc cacttcatct cgaagaattc cttcttcgga
      961 ccgtaaaggc ggaagagcag ctcgaactgt cctttcggat cggtcggcac ccagttgggc
     1021 tccttgccgg ccggagcctc ggggccgaaa tagatgtccg tcgatccgtc agcgttcttc
     1081 tgtaatgtcg tgtcgctcga cgcgaggctc gggcgggaca tgccggcgat cagcgcgtgc
     1141 gtcgcacgat cgtaggcggt caccgaccag tactgttcca tcggagcgtt cggtggaatg
     1201 gtcagccggt aggtcttgtt cccctccaac ggctggcccg agcggtcgct gagattcagg
     1261 agatagaact ggcctccgcc gaaggtcttc gggctgaagt aggcgaagtg gtaaaccacc
     1321 ccgcggccat cgatgccgta ttggttcggg tcggcaaagc cattgcccag acccgccacc
     1381 gtctctttcg gaatgggcaa tccccagcgc gtcccttcga agaacggcgt tgcgaaaccg
     1441 agttcgtatt tcagcgccag ctcggcgtgc gcttccttga cggccttctc caggatcgcc
     1501 ctggtcttcg cgtcgggctc gaagggcttt cccttttcga tgccgattgt cttgagcgaa
     1561 tcgatcatca ccttgtcgcg cgtgagccac ggctcggcct gcacgaagcg gttgaggtat
     1621 tcgaagaatg tggcgtcata cggaatcgtg gcgttgaacg gcttgtcata cacgtccacg
     1681 aacacggtcg agccggcgtc gccgccgaac ggatagacct tcaccttctt tccgtggtcc
     1741 acggcggact tgatgtcggc gtcgctgcgg ctcttgaagt tcgagcgcag gacgacgaag
     1801 ccgcggcagg tttccgatcg catcgcaatg tatccctcgg gcggctcgcc ctcgtagccg
     1861 ggcggcagga tgagatactt cgcgcccttg cccttgtcct cgccggccgg gccgacgtcc
     1921 atgagcgcgt tctgccagga gacgtcgaag ctgccgacga tgacgtgatc ggcggtggcg
     1981 ggcggaatct ccaccaccac cgggcctgtt gacgtgtcgt agaacgggtt gaggtagatc
     2041 gtgtcggggt tgggcgtgag agtccgggtc ctggaattga ccggacgcga ccagtagacg
     2101 acctggttca gtttcgcgcc gttgtcgatg gccgcctgca gcatgcgctc gtagttcacg
     2161 gcgggcatgc cccagatgac ggcctccacg gcgcggcggt ggatggcgcg atcgatgagt
     2221 tcctgtcgtg acttcatggg acgggggcta gcagaacgtg tgcctgatgg gtacgccgcg
     2281 gttgccgctg ggaagttgcc tgttggcgct gatctttcgt ccacgccatc tactcgaacg
     2341 acgtggtcac ccacaacgca ctgatactgc ccgtcgcggg aggtgagcgg ctggaaaagg
     2401 gtcgggtggt ggacgtcgag gcgaaggagg agctcgatac tcgctgatgc ggcgcctatg
     2461 aggctcgtga tgaacggccg gcccgcaact tgcggccggt gccggtcgcc cgttgcatac
     2521 gcttcttgcc tggattcggg atgtggaacg cgtagccgca ttcgaggcag cgccagtcgt
     2581 cgtgactctt ttcggtggcc atgca
//
LOCUS       DASUGB010000809         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1618795, whole genome shotgun sequence.
ACCESSION   DASUGB010000809 DASUGB010000000
VERSION     DASUGB010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618795"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFV87_15400"
     CDS             <1..812
                     /locus_tag="VFV87_15400"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5105205.1"
                     /translation="RYGLKGINYSVATACASANNAIGDAFKAIQHNDADVVITGGTEA
                     AITQMGLSAFQNMKALSTRNDDPPRASRPFDADRDGFVLSEGAGIFVLEEYERARRRG
                     AEILCEVLGFGASADAGHITQPDDQGTGAARAMSDALHDAGLNPADVGYINAHGTSTP
                     LGDKAETQAIKRVFGAHAKALSISSTKSQLGHSLGASGGIELVVTIKSLLDGVVPPTI
                     NLTTPDPDCDLDYTPNTPRERRFACAMSNSFGFGGHNASVVVGHLRNGAAR"
     gene            882..1550
                     /locus_tag="VFV87_15405"
     CDS             882..1550
                     /locus_tag="VFV87_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
                     /protein_id="HEX5105206.1"
                     /translation="MRSIRMVFICAALATCLPTGNAQNAARNKRFGAREGEVITPPAR
                     SERHQDSLGEGDAAPDFTLADPAGKSEFKLSEYRGKRPVVLVFGSFTCPPFRRRVLDV
                     DKLFEEFKDRVEFRFVYIREAHPDSVLFVKEGERESLQKIEQTETLDERGQHAKLCTE
                     TLKLAMPAVVDKADNAVNKTFAAWPIRLVIVDTEGKLAHISGPGPAGFNPSEVAAWLK
                     SNVK"
     gene            1652..3097
                     /locus_tag="VFV87_15410"
     CDS             1652..3097
                     /locus_tag="VFV87_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5105207.1"
                     /translation="MRAIRWYWFVSVGLGILAAAASAEDEFPYDAFVAVEDAEVVSGP
                     GHRYYATDRLPLGAKVEIYREEASGWLAIRPPEGSFSWIPGDAVERTNDESDVGRVTE
                     PTPCWIGTAAERVEEHRQHVELKVGEVVQILGEKQVADGDGEESTWLKIAPPAGEFRW
                     VHLRDVSRQKPEPVARRTSVIELNATTEPVDEPLSPREPERIELPREAIALRDLEPAG
                     DSRAGTPSRGIETAQFQSTSEPSGRLLSPDGFVPRKRRGSEPLQTVPEPSSRLARNGS
                     ATFTRPRLDPPPLMASVDSPRGTVKPTSSANSGDVTRKLEQLELELSLMLAQEKSTWN
                     LADLRQRIEELVPRGTDPVERGQARLLLDKVKAFEETFDVPDQPVATSASGRQPVAAT
                     GNSPLTDPRYDGEGWLVRVKSKTNRDVPPFALVDRDSNPLCFVTPAPGFNLSAYENRR
                     IGVFGRRGIMEETKKPHVLAERAIDLDKVLR"
BASE COUNT          625 a          971 c         1099 g          518 t
ORIGIN      
        1 ttcgctacgg cctgaagggt atcaattatt cggtggcgac tgcgtgtgcc agcgcaaata
       61 acgcgatcgg cgacgccttc aaggcgattc agcacaacga cgcggacgtt gtcatcactg
      121 gcggcaccga agcagcgata acgcagatgg ggctgtcggc cttccagaac atgaaggccc
      181 tttcgacacg caatgacgat ccgccgcgcg ccagccgccc gttcgacgcc gaccgcgacg
      241 gtttcgtgct gagcgaaggc gcaggcattt tcgtgctcga agaatatgag cgggccaggc
      301 ggcgcggagc ggagatcctg tgcgaagtgc ttggtttcgg cgccagcgcc gatgcgggcc
      361 acatcacgca gccggatgac cagggcacgg gcgcggcccg ggcgatgagc gatgccttgc
      421 acgacgcggg tctgaacccg gccgacgtgg gctacattaa cgctcacggc accagcacgc
      481 cattggggga caaggccgag acgcaggcga tcaagcgggt gttcggcgcg cacgcgaagg
      541 cgctatcgat cagctcgacc aagagccagc ttggccattc gctgggcgcc agcggcggaa
      601 tcgagctggt cgtcacgatc aagtcgctgc tggacggggt cgtgccgccg acgatcaacc
      661 tgacaacgcc cgatcccgat tgcgaccttg attacactcc caacacccct cgcgagcggc
      721 gattcgcgtg tgcgatgagc aacagcttcg ggtttggcgg gcataatgcg tcggtggtcg
      781 tcggccattt gcggaacggg gcggcgaggt aaacagcagg cggcgattct aaatctctcg
      841 cgctggggcg cgagaaagtg agcagaatcg tgagcggacg aatgcggtcg attcggatgg
      901 tgttcatttg cgccgcattg gccacttgcc tgccgaccgg aaacgcgcaa aacgcggccc
      961 ggaataagcg ctttggggcg cgcgaggggg aagtcatcac tccgccggct cgcagcgagc
     1021 ggcatcagga ttcgctgggc gaaggagacg cagcgcccga tttcacgctg gccgacccgg
     1081 cgggaaaaag cgagttcaaa ttgtctgagt atcgcggcaa gcggcctgta gtgctggtat
     1141 tcggcagctt cacgtgcccg ccattccgcc gccgcgtgct ggacgtcgac aagctgttcg
     1201 aggaattcaa ggaccgcgtg gaatttcgct tcgtctatat ccgcgaagcc catcccgatt
     1261 cggtgctgtt cgtgaaagaa ggcgagaggg aatcgctgca gaagatcgag cagaccgaga
     1321 ctctggacga gcgcgggcag cacgcaaagc tatgtaccga gacgctcaag ctggcgatgc
     1381 cggcggttgt cgataaggcg gacaacgccg tgaacaagac gttcgccgcc tggcccatcc
     1441 gcctggtgat cgtcgatacc gagggaaagc tggcgcacat cagcgggcct ggaccggcgg
     1501 ggttcaatcc gtcggaagtg gccgcgtggc tcaagagcaa cgtgaaatag tcgctagcga
     1561 cacatgctgg cacgcgtggt cttgagcgcg gtcagcgaaa cggctacaag tcgccccgcc
     1621 gaagtgggtt ggcgcttcgc gggaggcaac gatgcgggcg attcgctggt attggttcgt
     1681 ttctgttggc cttgggattc tcgccgcggc ggcgagcgcg gaggacgaat tcccttacga
     1741 cgctttcgta gccgtagagg acgcggaggt cgtcagcggt ccggggcatc gctattacgc
     1801 caccgaccga ctgccactag gggcgaaggt cgagatttac cgtgaagaag cctcgggctg
     1861 gctggcaatc cggccgccgg agggctcgtt cagctggata ccgggagacg cggtcgagcg
     1921 aacaaatgac gaatcggatg tgggacgcgt gaccgagccg acaccctgtt ggatcggcac
     1981 ggccgccgag cgggtcgagg agcatcgtca gcacgtggag ctgaaggtcg gcgaggtggt
     2041 ccagattctc ggcgagaagc aagttgcgga tggcgacggc gaggaatcaa cctggctgaa
     2101 gatcgctccc cccgcgggcg aattccgctg ggttcacctc cgcgacgtga gccggcagaa
     2161 gccggagccg gtcgcgcgac gaacgagcgt catcgaatta aatgcgacga ccgaacccgt
     2221 cgatgagcca ctctcgccgc gtgagcccga gcggatcgaa ctgccgcggg aagcgatcgc
     2281 cctgcgggac ttagaaccgg ccggcgacag ccgagctggc acgccaagcc gtggcatcga
     2341 gacggcccaa ttccagagca cgagcgagcc ctccggcagg ctcctttcgc cggacggctt
     2401 tgtgccgcga aagcggcgcg gaagcgagcc gctgcaaacg gtgcctgagc ccagttcgcg
     2461 actggccaga aacggatctg cgacgttcac gcgaccgcgg ctcgatccgc cgccattaat
     2521 ggcctccgtt gactcgccac gcggaaccgt caagccgacg agttcggcca acagcggcga
     2581 cgtcacccgc aagctcgaac aactcgagct ggagctgtcg ctgatgctcg cccaggagaa
     2641 atcaacgtgg aatcttgccg acctgcggca gaggatcgaa gagctggtcc cgcgcgggac
     2701 cgatcccgtc gagcgcgggc aggcccggct gttgctcgac aaggtgaaag cgttcgaaga
     2761 gacattcgac gtgccggatc agcccgttgc gaccagcgcc tccggccggc agcctgtcgc
     2821 ggccacgggg aactcgccgc tcaccgatcc gcgttacgac ggcgagggct ggctcgtccg
     2881 cgtcaagtcg aaaacgaatc gcgatgtgcc accctttgca ctcgtcgatc gggacagcaa
     2941 cccgctttgc ttcgttacgc ccgcgcccgg cttcaatctg agcgcctacg agaaccggcg
     3001 gatcggcgta ttcggacgcc gcggcatcat ggaggaaacg aagaagccgc acgtgctggc
     3061 cgagcgggcg atcgacctcg ataaggtgtt gcgctaggga tcgtccgaga tgcggatggc
     3121 cttgggcgct atgaactgac gcggcgtggc cggctgctct tggctggcgc cctccagcgg
     3181 ttctggttcc cgcggaaagg cgatcggcgg ctc
//
LOCUS       DASUGB010000810         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1061981, whole genome shotgun sequence.
ACCESSION   DASUGB010000810 DASUGB010000000
VERSION     DASUGB010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061981"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..262)
                     /locus_tag="VFV87_15415"
     CDS             complement(2..262)
                     /locus_tag="VFV87_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105208.1"
                     /translation="MYSPNEFWLNLHRLTEAYDAEGMTTAERFANITRQFQAMPPLAQ
                     QEILQELRRLTIHLPELYPVVAAEAADEPPQQPSLARREDVA"
     gene            573..2033
                     /locus_tag="VFV87_15420"
     CDS             573..2033
                     /locus_tag="VFV87_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5105209.1"
                     /translation="MNRISRRTVLRGAAAASAAFPLFTIAGTKASGKVIGANDTIRLG
                     VAGINGRGGAHISEFTGMGEKVRVTYLIDPDSRLFDSRAKAVMDRQAGLRPNTVQDIR
                     QALDDKELDAVTVATCNHWHSLITIWACQAGKDVYVEKPISHNVFEGRKCVEAAKKYN
                     RVVQHGTQSRSSPGWAKTIAAVYSGKYGKLLVSKGEASKNRWSIGFKPEVTPPATFDF
                     NLWLGPAPDQPFHGNLHPYNWHWFWDFGNGDIGNQGVHQMDIARWGIQGGTLPTKVYS
                     LGGRFLPDGPDQGQVPNQQLAVLEFGPVTLLFEVRGLTGRDKQWPNVVTNEFYTTDGV
                     IKEGGTFHPKDGGKAQKVEVDELPIAPGGAFGSFINAMRTRKPEDCNCDAEVAHFSAA
                     LIHLANASYRLGKQGTYDKARGAIGDNKEVVASLERIRDNTKAVGVPIDKTVYTIGPV
                     LTFDPASEKFTGERADEANKLITRQYRPPFIVPESV"
     gene            2249..>2914
                     /locus_tag="VFV87_15425"
     CDS             2249..>2914
                     /locus_tag="VFV87_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105210.1"
                     /translation="MSPQELSAALPEAALAFRGYNVTNLGRSGELLAHAAYGPTVERH
                     LHAISQAASQHLGRTVDLVSRVRGGVETSIESYGDAIALIIGMECAQRELMEQHFGIK
                     LRTAQFSFGYSLGEISALIGGGAIEAADALRIPLSLADDCVALAAEATLGVLFTRATE
                     LPLEDVREVCLRINAQGRGVIGISSHLAPNSVLLIGQHDTLDRFAELARGMTTERLHL
                     RKNE"
BASE COUNT          524 a          944 c          949 g          497 t
ORIGIN      
        1 ttcaggccac gtcttcgcgg cgggcgagcg acggctgctg gggcggctcg tcggccgctt
       61 cggccgcgac taccgggtac agctcgggca gatgaatcgt cagccgccgc agttcctgca
      121 gaatctcttg ctgagccagg ggcggcatcg cctggaactg ccgcgtgatg ttggcgaacc
      181 gctcggccgt cgtcatgcct tcggcgtcgt aggcctcggt cagccgatgc aggttcagcc
      241 agaactcgtt gggtgaatac acgaggagac ctccgtttca cgccagtgcg aggagtggga
      301 acttgcccat gcaattcatt tgcaaatgcg agtcgcgcct tatcttcctc cactttgggg
      361 acaatgcaag agtgcagtgg ggtattttcc acgccgatcg cttgccagtc gggtggacga
      421 atcgcccggc cgatgttgcc agcccgagcg gcacttgctg gactttggcc cagcgctgtg
      481 ctgtaatctg gctggcccat tcactacgtt tccccctccc ctacgctccc cgccagcatt
      541 ccatccctcg tttcccactt taggagcctg ccatgaaccg catttcccgc cgcaccgtgc
      601 tccgcggcgc cgcagccgcc agcgccgcct tccccctgtt tacgatcgcc ggcaccaagg
      661 cctcgggcaa agtgatcggc gccaacgata cgatccgcct gggggtggcc ggcatcaacg
      721 gccgcggcgg ggcccacatc agcgaattca ccggcatggg cgagaaggtg cgggtgacgt
      781 acctgatcga tcccgacagc cgcttgttcg attcgcgggc caaggcggtg atggaccggc
      841 aggcggggct gcggccaaac accgtgcagg acatccgcca ggctctggac gacaaggagc
      901 tggacgcggt cacggtggct acctgcaacc actggcattc gctgatcacc atctgggcct
      961 gccaggcggg caaggacgtg tacgtcgaaa agccgatcag ccacaacgtt tttgaggggc
     1021 ggaagtgcgt cgaagcggcc aaaaaataca atcgcgtcgt ccagcacggc acccaaagcc
     1081 gcagcagccc cggctgggcc aagacgattg ccgcggtcta ttccggcaaa tacggcaagc
     1141 tcctcgtgag caagggcgag gccagcaaga accgctggag catcggcttc aagccggagg
     1201 tgacgccgcc ggccacgttc gatttcaacc tctggctagg gcccgcgccc gaccagccgt
     1261 tccacgggaa cctgcaccct tacaactggc actggttctg ggactttggc aacggcgaca
     1321 tcggcaacca gggggtgcac cagatggaca tcgcccgctg gggcatccag ggcgggacgc
     1381 tgccgaccaa ggtttatagc ctgggcggcc ggtttttgcc cgatgggccc gaccaagggc
     1441 aagtgcccaa ccagcagctc gcggtgctgg aatttgggcc cgtgaccctg ctgttcgagg
     1501 ttcgcggcct caccggccgc gacaagcagt ggccgaacgt cgtcaccaac gagttctata
     1561 cgaccgacgg cgtgatcaag gaaggtggca cgttccatcc caaggacggt ggcaaggcgc
     1621 aaaaggtcga ggtggacgag ctgcccatcg ctcccggcgg ggcctttggc agcttcatca
     1681 atgcgatgcg cacgcgcaag ccggaggatt gcaactgcga cgcggaagtg gcccacttct
     1741 cggcggcgct cattcacctg gcgaatgcgt cgtatcgcct gggcaagcag ggcacgtacg
     1801 acaaggcccg cggggcgatc ggcgacaaca aggaggtggt ggcgagcttg gagcggattc
     1861 gcgacaacac gaaggcggtg ggcgtgccga tcgacaagac ggtctatacg atcggcccgg
     1921 tgctcacgtt cgatcccgcc agtgagaaat tcaccggcga gcgggccgac gaagccaaca
     1981 agctgatcac ccggcaatac cgtccgccgt tcatcgtgcc cgagtcggtg tagcgagaag
     2041 gcaactgcgc gacgacggca cagtgcgcaa agcggcagat ggaactggcg cttgcgccat
     2101 tgcgttgatc gggcactggc gacacgccag tggcacccgc ctgttgcgcc attgcgtttt
     2161 atgacgtagg cttgcgttag tggcgtgggt ccgtcggtca actgggatcc atccgtcaat
     2221 cagcccttcg caagcctccg gtcgctgtat gtctccccag gaattgtccg ccgcgttgcc
     2281 ggaagcagct ctggcgtttc gcggctacaa cgtgacgaac ctgggccgca gcggcgagct
     2341 gttggctcac gcggcctatg gtccgaccgt cgagcggcac ttgcacgcca tctcacaggc
     2401 ggccagccag catctcgggc ggacggtgga cctcgtatcc cgcgtgcggg gcggcgtcga
     2461 aacctcgatt gagtcctacg gcgacgccat cgccctcatc atcggcatgg aatgcgccca
     2521 gcgggagctg atggagcagc attttggcat caagctgcgg acggcccagt tcagcttcgg
     2581 ctacagcctg ggagaaatct cggcgctcat tggcggcggg gcgatcgaag cggccgacgc
     2641 cctgcggatt ccgctctcgc tggccgacga ctgcgtggcc ctggccgccg aggccacgct
     2701 gggcgtgctc ttcacgcggg ccacggagtt gccgctggag gacgtgcgcg aagtctgcct
     2761 gcgaatcaac gcccagggcc gcggcgtgat cggcatctcg tcgcacctgg cgccgaactc
     2821 ggtgctgctc atcggccagc acgacacgct cgaccgcttt gccgagctgg ctcgcggcat
     2881 gaccaccgag cggctgcacc tccgcaagaa cgag
//
LOCUS       DASUGB010000811         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2034861, whole genome shotgun sequence.
ACCESSION   DASUGB010000811 DASUGB010000000
VERSION     DASUGB010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034861"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFV87_15430"
     CDS             <1..457
                     /locus_tag="VFV87_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5105211.1"
                     /translation="WPSTEPILEVTLPSSVDRTIAPEGRHVMNIFVQYAPYRLAAGKS
                     WDDIKEDFADRCIELLARYAPNVPQSVLHRQILSPLDLERTFGLTGGNIMQGAMSPNQ
                     LFALRPVAGWADHRTPIRGLYLCGAASHPGGGVLGACGRNAAEEILRDG"
     gene            complement(454..834)
                     /locus_tag="VFV87_15435"
     CDS             complement(454..834)
                     /locus_tag="VFV87_15435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5105212.1"
                     /translation="MNSASSESGQLPQQLQTEREFFGKSGPLPAAWNELRRYPRFYLR
                     SFAQARIFPPGGDEPPKQCTILTCDISRGGMAFLYKKQLFPGQRIDVMLHDGAERSVE
                     VQWCRRIGDGCYWAGGRFIKEAAN"
     gene            complement(831..1790)
                     /locus_tag="VFV87_15440"
     CDS             complement(831..1790)
                     /locus_tag="VFV87_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5105213.1"
                     /translation="MLDDSPEILGYLPVATATLCPATVMGCELFIQRGTSSLVELFKG
                     ANYPLDVTDVERLRTAGIDHLYVRLEAADAYRDYLRENVLQDDALPAGARVKALREVT
                     RVVFEQALSRGNAGELVQTAVEFGRGMATMMSEKSLLFREIFRLIEHDFYTFTHVCNV
                     STYCVMLAQALGETNADRLTELAAAGLLHDVGKRHIPAAVLNKPGRLTPAEWELVVRH
                     PADGYLELKQQKLSWGQLMVVYQHHERMDGSGYPTGVRGDEIHPWAKICGLVDVFDAM
                     TCKRPYRKAIAIDDVCSHLNRQAGVTFETEIVNCWLAQVRRKS"
     gene            2031..>2291
                     /locus_tag="VFV87_15445"
     CDS             2031..>2291
                     /locus_tag="VFV87_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105214.1"
                     /translation="MNLPQAKSPAARPVAARPKLAHNPSRPWQFSLGSLLCLLVGLGS
                     MLAAALPQGMYAPVIGWAILAAIYWRQGWRDLLFVHGVLPAIS"
BASE COUNT          415 a          736 c          685 g          455 t
ORIGIN      
        1 ttggccgagc accgagccga tcctggaagt cacgctccct tcgtccgtcg atcgcacaat
       61 cgcgccggag ggccgacacg tgatgaacat cttcgtgcag tacgcgccgt ataggctggc
      121 ggcagggaag agctgggacg atatcaagga agattttgcc gatcgctgca ttgagctgct
      181 ggcccgttat gcccccaatg tgccccagtc ggtcctgcac cggcagattc tcagcccgct
      241 tgatctggag cggaccttcg gtctgaccgg cggcaacatc atgcaggggg cgatgtcccc
      301 caaccagctc ttcgcgttgc gccccgtcgc gggctgggcc gaccatcgca cgccgattcg
      361 cgggctgtac ctctgcggcg cggccagtca tcccggcggc ggagttctgg gcgcctgcgg
      421 ccgcaatgcc gccgaagaaa ttctccgcga cggttagttc gccgcttcct tgatgaaccg
      481 cccgccagcc caatagcagc cgtcgccgat ccgccggcac cattggactt cgaccgagcg
      541 ttcggctccg tcgtgcagca tcacgtcgat tcgctgtccg ggaaagagct gttttttgta
      601 caggaaggcc attccgccgc gggagatatc gcacgtcagg atcgtacact gcttgggcgg
      661 ctcgtctcct cccggcggga agattctggc ttgggcaaat gaccgcaaat agaatcgcgg
      721 gtagcggcgc agctcgttcc atgcagccgg cagcgggccg gatttgccga agaactctct
      781 ttccgtctga agttgctgcg gcaactggcc actctcgctg gaagcggaat tcacgacttc
      841 ctccgcactt gcgcaagcca gcagttcacg atttccgtct cgaacgtaac acccgcttgc
      901 cgattcagat gagaacacac gtcgtcgatc gcaatcgcct tgcgatacgg ccgcttgcac
      961 gtcatcgcgt caaacacatc gacgagtccg cagattttcg cccagggatg aatctcgtcg
     1021 ccgcgcacgc ctgtgggata gccgctgccg tccattcgtt catggtgctg atagacgacc
     1081 atgagctggc cccacgagag cttttgctgc ttcagctcca ggtagccgtc cgccggatgc
     1141 cgcaccacga gttcccattc ggccggcgtc aggcgaccgg gcttgttcag caccgcggcg
     1201 ggaatgtgcc gcttcccgac atcgtgcaac agtcccgcgg ccgccagctc cgtaagccgg
     1261 tcggcgtttg tctcacccag cgcctgggcc agcatgacgc agtaggtgct gacgttgcag
     1321 acatgcgtga acgtatagaa atcgtgctcg atcagccgga agatctcgcg gaacaagagc
     1381 gacttctcgc tcatcatggt cgccatgccg cggccgaact cgacggcggt ttgcaccagc
     1441 tcgccggcat tgccgcggga caaggcctgc tcgaagacga cgcgcgtcac ttcccgcaag
     1501 gctttgacgc gagcgccggc cggaagcgca tcgtcttgca gcacgttctc ccgcaaataa
     1561 tcgcgatagg cgtcggcagc ttccaggcgg acatagaggt ggtcgattcc ggccgtacgc
     1621 aaccgctcga cgtccgtcac atccagcgga tagttggcgc ccttgaacag ctcgaccagt
     1681 gacgaagtcc cgcgctgaat gaacagctcg cagcccatca ccgtggccgg gcagagtgtg
     1741 gccgtagcaa cggggagata acccagaatt tcgggagagt cgtccaacat caggcactcc
     1801 gctcagcgcg catggcgctc cgaaaaaacc tacgataggg gcgcggacac tttcctgctt
     1861 ttgggcggga atccgccgaa aaatgtcacc gcggaaccaa cacaggcgat gcaattgcag
     1921 cagggtagcc tcggcggcat aatcggtcct gcgatgaatt cttgctttat tctccgctca
     1981 ggtggttctg tggcagatcc gcgcgctttt ggattttggc tttgcctcgc atgaatctac
     2041 cgcaggcaaa atcgcccgcc gctcgccccg ttgctgcgcg accaaaactg gcacacaatc
     2101 cttcgcgtcc ctggcaattc tcgctcggct ctctcctgtg cctgctcgtc ggcttgggga
     2161 gcatgttggc ggcagctttg ccgcaaggga tgtatgcccc ggtcatcggc tgggcgattc
     2221 tggcagcgat ctattggcgg caaggctggc gggacctgtt gttcgttcac ggcgtcttgc
     2281 cggcgatctc a
//
LOCUS       DASUGB010000812         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1618952, whole genome shotgun sequence.
ACCESSION   DASUGB010000812 DASUGB010000000
VERSION     DASUGB010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618952"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..736)
                     /locus_tag="VFV87_15450"
     CDS             complement(278..736)
                     /locus_tag="VFV87_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX5105215.1"
                     /translation="MCRRIFIAFLALSITAPLAAEEPQPATAESEMQQLAGTWEGYAV
                     EDRGERPDRGPVHLRLVIDEGKIRAVDLGSPEKKDMGSGTYKLDPSQKIKQIDATGII
                     LPGKRERTFLGIYEIDGDTFKWCVDNRSKERPTEFRTIGGKYLLILKRQK"
     gene            complement(827..2761)
                     /gene="cysN"
                     /locus_tag="VFV87_15455"
     CDS             complement(827..2761)
                     /gene="cysN"
                     /locus_tag="VFV87_15455"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX5105216.1"
                     /translation="MSHQSALIATDINEYLAQHERKELLRFITCGSVDDGKSTLIGRL
                     LYESKMIYEDQLAAIQKDTQKYGTTGGELDLALLTDGLEDERQQGITIDVAYRYFSTD
                     KRKFIIADTPGHEQYTRNMATGASTADLAIILIDARHGVLVQTKRHSFIVSLLGIKHI
                     VVAINKMDLVDFSEEVFNKIREDYQAFAARLDLPDVHFLPMSALNGDNVVQQSQHTPW
                     YVGPTLMSLLENVYIGSDRNLEDFRFPVQSVLRPNLDFRGFAGTIASGIVRQGDEVMA
                     LPSRKTSRIERIVTFDGDLQEAFAPQAVTLTLEDEIDVSRGDMLVRPGNVPRVEQKFE
                     AMIVWMSEDPMVPGKSYWIKQTSKLTPGTISTLRYQIDVNTLHRKDAPTLRLNEIGRC
                     SLTTSQPLCFDAYRRNRSTGALIVIDRLTNSTVGAGMILDRASEGGRHDHWDDEPAAA
                     TLHGERSNVSSDEREARFGQKPVTVLFTGLTGSGKTTLAFALERKLFDQGRAVVVLDG
                     QNMRRGISKDLGFTSDDRSENLRRSAEVARLFNDAGILVIAAFLAPDEAVRQKAADCI
                     GRERFLVVHLDCPLETCRQRDTEGHYKLADSGEIALFPGVTSPYEPPAGPDLKLATDK
                     LSVAECLERVLDLLDTKGVA"
     gene            complement(3040..3945)
                     /gene="cysD"
                     /locus_tag="VFV87_15460"
     CDS             complement(3040..3945)
                     /gene="cysD"
                     /locus_tag="VFV87_15460"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX5105217.1"
                     /translation="MSGYSLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSACML
                     RLAEKAFPPPAKIPFPLLHVDTTWKFREMYELRDRVARQHKLLIHVNEEGLKKGISPI
                     THGSKVHTDVMKTEGLKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDQFHRWDPK
                     NQRPELWNLYNARVNKGESIRVFPLSNWTELDVWQYIHLENIPLVPLYFAQERPIVWR
                     DGMMILVDDDRLPLQPGEKPELRRVRFRTLGCYPLSGAVDSNATTLPEIIQEMLLTKS
                     SERQGRLIDHDQSGSMEKKKKEGYF"
     gene            complement(4065..4484)
                     /locus_tag="VFV87_15465"
     CDS             complement(4065..4484)
                     /locus_tag="VFV87_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX5105218.1"
                     /translation="MPSPEENPTADIVIYDGHCKFCTGQVRNLARWDRSGKRLAFLSL
                     HDPEVAKRFPDLTYEQLMEEMYVVDQKGRHHAGADGFRYLTTRLPLLYPLAPVMHIPF
                     TRPLWRWGYRQVAKRRYRIMGKTGECDDGACAVHFKK"
BASE COUNT          787 a         1312 c         1478 g          929 t
ORIGIN      
        1 gttagcgcgc aaaccgccag cgcaagtgca agaaccaggc gtttcgctga gaaaccgagc
       61 ataagtcacc tcccaagcga tggtgagcat tgacgggcac ggcagcgcga acatgcactc
      121 cgccaaagca agtgcggtcg atgctaattc gtggtccgcg gggcgtcaac caacaacgcc
      181 ctcagcatga atagcgccgg cgatcaccgt cgcgagttcc cgccgcctgc cggacgtttc
      241 tctcgctccc ttgctcccag ggccgtggtg gcccacttca cttctgccgc ttgaggatca
      301 gcagatactt gccgccgatc gtgcggaact cggtggggcg ctctttcgac cggttatcga
      361 cgcaccactt gaacgtgtcg ccgtcgattt catagatgcc gaggaacgtg cgctcgcgct
      421 tgcccggcag gatgatgccc gtggcgtcga tctgcttgat cttctggctg ggatcgagct
      481 tgtaggtgcc gctgcccatg tcctttttct cggggctgcc cagatcgacc gcacggatct
      541 tcccctcgtc gatgaccagc cgcaggtgga ccggcccgcg atcggggcgt tcgccgcggt
      601 cctcgaccgc atagccctcc caggtgccgg cgagctgctg catctcggat tctgccgtgg
      661 cgggctgtgg ctcttccgct gccaacggag cggtgatcga caacgcgagg aacgcaatga
      721 agatgcgacg acacatgggg aagcttcggg cacgggaaag agtcgccagg acgcgattgt
      781 acggccggat cacggccgag tcagcgcagc gatgatcgag gccagcctaa gcgacgcctt
      841 tggtatcgag cagatcgagt accctctcga ggcactcggc gacgctgagc ttgtcggtgg
      901 cgagtttcag gtcgggcccg gcgggcggct cgtaggggct ggtgacgccg gggaagagag
      961 cgatttcgcc gctgtcggcc aacttatagt gcccttcggt gtcgcgctgg cggcaggttt
     1021 cgagcgggca gtcgagatgc acgacaagga accgctcgcg gccgatgcaa tcggcggctt
     1081 tctggcggac ggcttcgtcg ggggccagga acgcggcgat gaccaggatc ccggcgtcgt
     1141 tgaagagcct ggcgacctcg gcgctgcggc ggaggttttc gctgcggtcg tcggaggtga
     1201 agccaaggtc cttgctgatg ccgcggcgca tgttctgtcc gtcgaggacg acgacggcgc
     1261 ggccctggtc gaagagcttt cgctccaggg caaacgccag cgtcgtcttg ccgctgccgg
     1321 tgaggccggt gaagaggacc gtgacgggct tttggccgaa gcgggcctcg cgctcgtcgc
     1381 tactgacgtt gctccgctcg ccgtggagcg tcgcggcggc gggctcgtcg tcccagtggt
     1441 cgtggcggcc gccttcgctg gcccggtcaa ggatcatccc cgcgccgacc gtgctgttgg
     1501 tcaatcggtc gatgacgatc agggcgccgg tcgaacgatt gcggcgatag gcgtcaaagc
     1561 agagcggctg gctcgtcgtg agcgagcagc ggccgatttc gttgaggcgc agcgtcgggg
     1621 cgtccttgcg gtgcagcgtg ttgacgtcga tctggtaccg cagcgtgctg atcgtgcccg
     1681 gcgtgagctt gctggtctgc ttgatccagt acgacttgcc gggaaccatc ggatcttcgg
     1741 acatccagac gatcatcgcc tcgaacttct gttcgacccg cggcacattg cccgggcgca
     1801 caagcatgtc gccgcggctg acgtcgattt cgtcttcgag cgtcagcgtg accgcctgcg
     1861 gggcgaaggc ttcctgcagg tcgccgtcga acgtgacgat tcgctcgatg cggctggtct
     1921 tacggctcgg gagggccatc acttcgtcac cctggcggac gatgcccgac gcgatcgtcc
     1981 ccgcaaagcc gcggaaatcg aggttcggcc gcaggaccga ttgcacggga aagcgaaaat
     2041 cctccaggtt gcggtcagag ccgatataga cgttttcgag caggctcatc agcgtcgggc
     2101 cgacgtacca gggcgtgtgc tggctctgct ggacgacgtt atcgccattc aaagccgaca
     2161 tcggcaggaa atgcacgtcg ggcaggtcga ggcgggcggc gaaggcctgg tagtcctcgc
     2221 ggatcttgtt gaacacttcc tcgctgaaat cgaccaggtc catcttgttg atggcgacga
     2281 cgatgtgctt gatgcccagc agcgacacga tgaacgagtg ccgcttggtc tggacgagga
     2341 cgccgtggcg ggcgtcgatg aggataatcg ccaggtcggc ggtcgaggcc ccggtcgcca
     2401 tattacgggt gtactgctcg tggcccggcg tatccgcgat gatgaatttc cgcttgtcgg
     2461 tgctgaagta gcgatacgcg acatcaatcg tgattccctg ctgccgctcg tcttccaggc
     2521 cgtcggtcag cagggccaaa tccaactcac cgcccgtcgt gccatatttc tgcgtatcct
     2581 tctgaatcgc cgcgagctga tcctcgtaga tcatcttcga ttcatagagc agccggccga
     2641 tgagggtgct cttgccgtca tcgacggagc cgcacgtgat gaacctgagg agttccttgc
     2701 gctcgtgctg ggcgaggtat tcgttgatgt cggtggcgat gagggcggat tggtgggaca
     2761 ttcgactact tcgttgttga ctaacgcggc tcaaagcggc tgttgatgac gtctccattg
     2821 gcgtcatcaa tcacaaccac aactcgacag ccgatacctc ggtgtgacaa caccgtccaa
     2881 gtttttcgcc gtcgatgaaa caataggcgc gtcaaccaac cggcttgctt ggcatatcca
     2941 ggtccgatcc aagttcagcc attgtcgttt gccacgcttt tggctttttc gattgcatcc
     3001 acttcggtca tagttacaca ccaacgtcga ccactgcgat cagaaatacc cttccttttt
     3061 cttcttctcc atcgacccgc tctggtcgtg gtcaatcaat cgcccctgcc gttcggacga
     3121 tttggtcagc agcatttcct ggatgatttc gggcagcgtc gtcgcgttgg aatcgaccgc
     3181 gccagaaagc ggatagcagc cgagggtgcg gaaacggacg cggcggagtt cgggcttttc
     3241 gccgggctgg agcgggaggc gatcgtcatc gacgaggatc atcatgccgt cgcgccagac
     3301 gatgggccgc tcctgggcga agtagagcgg gacgagcggg atgttttcga gatggatgta
     3361 ttgccagacg tcgagctcgg tccagttgga gagcggaaag acgcggatgc tctcgccttt
     3421 gttgacgcgg gcgttgtaga ggttccacag ctcgggccgc tggttttttg gatcccagcg
     3481 atggaactgg tcgcggaacg aatagacccg ctccttggcc cgcgattttt cttcgtcgcg
     3541 gcgggccccg ccgaaggcgg cgtcgaattg gtatttgtcg agcgcctgct tgaggccctc
     3601 ggtcttcatc acatcggtgt ggaccttgct gccgtgcgta attggactta tccccttttt
     3661 cagtccctcc tcgttgacgt ggatgagcag cttgtgctgg cgggcgaccc ggtcgcgcag
     3721 ctcgtacatc tcccggaatt tccaggtggt atcgacgtgc aacagcggaa acgggatttt
     3781 cgccggcggc gggaaggctt tctcggccag ccgcagcatg caagccgagt ccttgccgat
     3841 cgaatagagc atgaccgggt tttcgaactc cgccgccacc tcgcggatga tgtggatgct
     3901 ctcggcttcg agctgcttga ggtgggtgag ggagtagccg gacattcagg caaggggaca
     3961 ggggacaggg gacaggggac agggccctgc tcttttgaca atatagagga gttatgccgg
     4021 ttgaccaacg gcggagagat tgggcgcaaa gtgttgcggg gcaatcactt cttgaagtgg
     4081 acggcgcacg cgccgtcatc acattcgccc gtcttgccca tgatgcgata gcggcgtttg
     4141 gcgacctgcc gatagcccca gcgccagagt ggccgcgtaa aggggatgtg catgaccggc
     4201 gcgagcggat agaggagcgg caggcgcgtc gtgagatacc gaaatccgtc cgctccggcg
     4261 tggtggcggc ccttttgatc gacgacgtac atttcctcca tcaattgctc gtaagtcaaa
     4321 tcggggaaac gctttgcgac ctccggatcg tgaagcgaga gaaacgccag tcgcttgccg
     4381 gatcgatccc agcgggcgag gttccgaacc tgtccggtgc agaacttgca atgaccgtca
     4441 taaatcacaa tgtcggcggt ggggttctcc tccggcgatg gcaaatggat tttcggctga
     4501 tcggcg
//
LOCUS       DASUGB010000813         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1062136, whole genome shotgun sequence.
ACCESSION   DASUGB010000813 DASUGB010000000
VERSION     DASUGB010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062136"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFV87_15470"
     CDS             <1..549
                     /locus_tag="VFV87_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5105219.1"
                     /translation="LRPTLPGDWAATLVAVDGNVVVDVPRQLERLPPDATHLTISAGG
                     NDALGCASILGQPAGSVAEVVRELADIQDTFRREFREMLDAVLRKNLPTLVCTIYDAI
                     PDLPREALAALSFFNDVITREACRAAVPVLDLRVLCRDRADYSSLSPIEPSALGGAKI
                     AAALAHFITTHDFSRRECVLFC"
     gene            662..1507
                     /locus_tag="VFV87_15475"
     CDS             662..1507
                     /locus_tag="VFV87_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEX5105220.1"
                     /translation="MSDHSKIEWTDATWNPVRGCTKISPGCKHCYAETFAERFRGVPG
                     HPYEQGFDLRLVPEKLLEPLKWSSSKMVFVNSMSDLFHEGVSDEYIEWCAKVMVAANW
                     HTFQVLTKRSERLRDLLLGRLQFAAHHDHIWWGVSVENRKHGLPRIEHLRAAQTKVPF
                     LSIEPLLEDLGELNLNGIRWVIVGGESGHGARPIVRDWVVCIRKQCHRTGVPFFFKQW
                     GGIQKSKTGRKLDGRTYDGFPSHEFHAPLDRIARLTLVDEFERDVSEWLKIAKRAKAD
                     WRAAG"
     gene            1515..2411
                     /gene="tcmP"
                     /locus_tag="VFV87_15480"
     CDS             1515..2411
                     /gene="tcmP"
                     /locus_tag="VFV87_15480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEX5105221.1"
                     /translation="MTSSTVGPWARKKLKRLRDYLSAYAVIMAKQQRDGWLKGFHYID
                     AFAGPGSHVERPRAAPNVLQQLFAEVARYSAQYPEQRAFLAGSPRVALDIQPEFSACY
                     FVEKSDDRVASLQELKKEFPKRRIEIRQMDCNEFLRQVVNTKPSFWRRNRAVAFLDPF
                     GMQVPWNTIELLGQTQAIDILVNFPVGMAIQRLLLRSGEFTKPQRRKLDEYFGSPDWF
                     HEIYKKDRNLWGDEETVKVEKSGEALVKWYQKRLKGAFGYVSRASLIRNTRRFPLYYL
                     ILASPKPIAAKIANHILDSDED"
     gene            complement(2408..>3334)
                     /locus_tag="VFV87_15485"
     CDS             complement(2408..>3334)
                     /locus_tag="VFV87_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5105222.1"
                     /translation="EFYGTVANTPFFNPPNFIDSRVGLHLQPVSDPSFYTTDVYAERA
                     CDWIEKNKKGPFFLYLPFNAQHAPLQATEKYLSRFGNIEDEKRRTFAAMMSAKDDAVG
                     RVLAKVKEIGAEDNTLVWYLADNGGPTASTTSMNGPLRGFKAQTWEGGTRVPFMVKWP
                     GKLPAGKVYENPIIQLDILPTCLHAAAAQPDAPQKPLDGVNLLPYLTGASTDRPHQTL
                     YWRFGPQWAVRHGDYKLVKGAGQDAPGLYNLAADIGESKDLSADEPSKVTELKALWDK
                     WNAEQSEPKWQPNPNAKAKKKGAKKAKAKAVN"
BASE COUNT          655 a         1023 c          983 g          673 t
ORIGIN      
        1 ctcaggccaa cactgcccgg cgattgggcc gccaccttgg tcgccgtgga cggcaacgtg
       61 gtcgtggatg tgccacgcca gttggagcgg ttgcccccgg atgcgacgca tctgaccatc
      121 agcgccggcg gcaacgacgc gctgggctgc gccagcatct tgggtcaacc ggccggcagc
      181 gtggcagagg tggtccgcga attggccgat attcaagaca cctttcgccg cgaatttcgc
      241 gagatgctgg acgccgtgct aaggaagaac ctccccaccc tcgtctgcac gatctacgac
      301 gcgattcccg acctgccgcg ggaagccctc gcggccctct cgttcttcaa cgacgtcatc
      361 acccgcgagg cgtgccgggc ggcggtcccc gtgctcgacc tccgcgtcct ctgccgcgac
      421 cgggccgact actcctccct ttcgcccatc gagccctccg ccctcggcgg cgcaaaaatc
      481 gccgccgccc tcgcccactt catcaccacc cacgacttct cccgacgcga gtgcgtgctg
      541 ttttgctaac atccggttgg gagccgagat tggcatcggg cctgccggcg gcgattccac
      601 cttcttggcc ttgtcgctgc gactgtacac cggtttataa tcgggggagg aggcccctga
      661 catgagcgat cattccaaaa tcgaatggac cgatgccacg tggaatcccg tccggggctg
      721 tacaaagatc agcccgggct gcaagcactg ctatgccgag acatttgccg agcggtttcg
      781 tggcgtcccc ggccacccct acgaacaggg cttcgatctg cggctcgttc cagagaaact
      841 gctggagccg ctgaagtggt catcgtcgaa gatggtcttc gtcaactcga tgagcgacct
      901 cttccatgaa ggcgtgagcg acgagtacat cgaatggtgt gccaaagtga tggtggcggc
      961 caattggcac acctttcaag tcctcaccaa gcgttccgag cgcctgcgag acttgctcct
     1021 cggccggctg cagttcgccg cccatcacga ccacatctgg tggggcgtca gcgtcgagaa
     1081 tcgcaagcac ggcctcccgc gaatcgaaca cctgcgcgcc gcccaaacca aggttccatt
     1141 tctctccatt gagccgctgc tggaagacct cggcgaactg aatttaaacg gcatccgctg
     1201 ggtcatcgtc ggcggcgaaa gcgggcacgg agcgcgaccc atcgttcgtg actgggtcgt
     1261 ctgcattcgc aagcaatgcc atcgaactgg cgtgccattt ttcttcaagc agtggggtgg
     1321 aattcaaaaa tccaagacag gacgaaagct tgacggaaga acctacgacg gatttccgtc
     1381 gcacgagttc cacgctccac tcgacagaat tgcgaggctg acgttggtgg atgaattcga
     1441 acgagacgtg agtgaatggt tgaaaatcgc caaaagagcg aaggccgatt ggcgcgccgc
     1501 tgggtaacaa gctgatgacg tcgagcacag tcggaccctg ggcaaggaag aagctgaagc
     1561 ggcttcgtga ctacctttcg gcttatgcgg tcatcatggc caagcagcaa cgcgacggtt
     1621 ggctcaaggg ctttcactat atcgacgcat ttgctggtcc gggatcgcac gtcgagcgtc
     1681 cacgcgccgc acccaatgtg ctgcagcagc tattcgccga agtcgccaga tattcagctc
     1741 agtaccccga gcaacgagca tttcttgctg gatcgccgcg cgtagcgctt gacatccagc
     1801 ctgaattttc ggcctgctac ttcgtcgaaa agtcggatga ccgagtggca agccttcaag
     1861 aactcaagaa agagtttccg aaacgcagaa tcgaaatccg ccagatggac tgcaacgagt
     1921 tcctgcgaca ggtagtgaat acaaaaccgt cattctggcg gcgaaaccga gcggtcgcct
     1981 tcctcgatcc gttcggcatg caagtcccgt ggaacacgat cgaattgctc ggccagactc
     2041 aagccatcga catcctcgtg aatttccccg tcggaatggc aattcagcgg ctcctgctgc
     2101 gaagcgggga gttcacgaaa cctcagcgac gaaaactcga cgaatacttc ggttctcctg
     2161 actggtttca tgaaatctac aagaaggacc gaaacttgtg gggagacgaa gaaacggtca
     2221 aggtggagaa atcgggcgag gcactggtta agtggtatca aaagcgattg aagggcgcat
     2281 tcggatacgt ttctcgcgcg tcactgattc gcaacacgcg ccgatttccc ctgtattacc
     2341 tgatcttggc ttcgccgaag cccatcgcgg cgaagatcgc aaatcacatt cttgattcgg
     2401 acgaagacta gttcaccgcc ttcgccttcg ctttcttcgc ccccttcttc ttcgctttcg
     2461 cgttgggatt cggctgccac ttgggttcgg attgttcggc gttccattta tcccacaggg
     2521 ctttcagctc ggtgaccttg gagggctcat ccgccgagag gtctttcgac tcgccgatgt
     2581 cggcggccag gttatacagg ccaggggcgt cctggccggc gccctttacg agcttgtagt
     2641 cgccgtggcg gacggcccac tgcggaccga atcgccaata aagcgtctga tgcggacggt
     2701 ccgtgcttgc gcccgtgagg tacggcagga ggttgacgcc gtccagcggc ttctgcggag
     2761 catcgggctg ggcagcggcc gcgtgcaggc acgtcggcag aatgtcgagc tggatgatcg
     2821 ggttctcata aaccttgccg gccggcagct tgccgggcca cttgaccatg aacggcacgc
     2881 gtgtgccccc ctcccacgtc tgcgccttga atccgcggag cgggccattc atcgacgtgg
     2941 tgctggcggt gggtccgcca ttgtcggcca gataccagac gagcgtgttg tcctccgcgc
     3001 cgatctcctt gaccttggcc agcacgcggc cgacggcgtc gtccttggcc gacatcatcg
     3061 ccgcaaacgt ccgccgtttc tcatcctcga tgttcccaaa gcggctcagg tatttctcag
     3121 tcgcctgcaa gggcgcatgc tgggcgttga acggcagata gaggaagaac ggacccttct
     3181 tgttcttctc aatccagtcg caggcccgct cggcatagac gtcggtcgtg tagaagctcg
     3241 ggtcgctgac cggctgcagg tgcaggccca cacggctgtc gatgaaattc ggcgggttga
     3301 agaagggggt gttggcgacc gtgccataga actc
//
LOCUS       DASUGB010000814         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1062144, whole genome shotgun sequence.
ACCESSION   DASUGB010000814 DASUGB010000000
VERSION     DASUGB010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062144"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VFV87_15490"
     CDS             complement(<1..1435)
                     /locus_tag="VFV87_15490"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX5105223.1"
                     /translation="MNALLAPLGWWLVDFAWLATVLLAAAVVLRWVVRDPAGRVRLAW
                     GAWLAIACAAVVTAWPAWPRLRVQDVVGRPARAIPSATQAIAPPLDLPATMVLIQEPG
                     EPLVPTPAARAPASLPAPPLSLPKLAVVGWLSGVFSAIGWVLIGWWRMRRLMGRTQPA
                     ADWIAAELAGITGRGQRSPRVRTSERLETAVAVGALAPRIVLPHSTAVPANALLVRAA
                     LAHEWAHIQHGDLWLLALERSLLPLLALHPLFWWLRRGTRLDREMWADAAAAGSKPVE
                     YAEALVAWAKGSRPHAGLAALSMTERPGNLTRRVNMILQAKHIPQSRADRLVGLTALA
                     ILAAISAALSLVTVRPTTAQDEPAPELAPSDAQAADAIRSYPLIGPARLTRTLPAPPA
                     EGPAIMVELHLLSFDSQAAADAKIELPTTLQPGEQGTEGVGSAELDQREAQRLMESVL
                     GTKGAVMLSEPKLVTLSGREANLVVGTT"
     gene            complement(1432..1854)
                     /locus_tag="VFV87_15495"
     CDS             complement(1432..1854)
                     /locus_tag="VFV87_15495"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5105224.1"
                     /translation="MPMEIVPSERELQALKVLWQAGEATVREITAAINEQGESLAYTT
                     VLSLLQVMEQKGLVSHRASGKFYLYFARVRKGPTVRALARGFLDRVFDGAVDEYLVHA
                     LEAKRPSDEEIRRLEELVAQAKHGRHAARTKRKSEGLK"
     gene            complement(2011..>3186)
                     /locus_tag="VFV87_15500"
     CDS             complement(2011..>3186)
                     /locus_tag="VFV87_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105225.1"
                     /translation="KKEAEFKGAGIDALEAQNRQRESLARRGVVPAAPPAPAAAGRPG
                     DFGYLAKADEKARTLQLQQGVQSVAQASDVGEMFRYVIATPVKLARGKSAMLPIVNES
                     VQGEKVSIYNEATHPKHPLNGLKLKNSSELHLMQGPITVFDDNAYAGDAQIMDLPPGS
                     ERLISYAMDLDTEVAPTQKDDPRRLTAVRIAKGTLHSSHKYARTKSYTVKNSGKRDKT
                     VLVEYPIQQPWNLVEPKDPAEKTRSQYRFALKAEPGKPATLDVKEEYTQSEQIAMTNL
                     DSGTIAYYLNQPVVSEAVKKALQEVVTRQAAIQQVAQQRQEMERQIAVIDAEQKRIRE
                     NMAQLGKETELYRKYVTKLTEQEDSNDDLRKQITAAVAEEARLKKALDDYLLGLEIG"
BASE COUNT          511 a         1052 c         1020 g          603 t
ORIGIN      
        1 acgtggtgcc cacgacaaga ttcgcttcgc ggccgctcag cgtgaccagc ttaggttccg
       61 agagcatgac ggcccccttg gttcccagca cggattccat cagccgctgc gcttcccgtt
      121 ggtccagttc cgcactgccg accccctccg ttccttgttc accaggttgc aatgttgtcg
      181 gcagttcgat cttggcgtca gccgcggcct gcgaatcgaa tgacaacagg tgcaattcga
      241 ccatgatcgc ggggccttcc gccggcggcg cgggaagcgt gcgagtgagc cgcgcgggac
      301 caatcaaggg ataggagcgg atcgcatcag cggcttgtgc atcggaggga gccaattccg
      361 gtgccggttc gtcctgggca gtggtggggc ggacggtcac cagggaaagc gcagccgaaa
      421 tggctgccag aatggccagc gccgtcaggc cgacgagccg gtcggcgcgt gactgcggaa
      481 tatgtttcgc ttgcaggatc atgtttactc tccgcgtaag gttgcctggt cgttcggtca
      541 tggacaaggc agcgaggccc gcgtgggggc ggctgccttt cgcccaggcc acaagggcct
      601 cggcatattc gacgggcttg ctgccggcgg cggcggcgtc ggcccacatc tcccggtcca
      661 gccgcgttcc gcgccgcagc caccagaaga gcggatgcaa ggccagcagc ggcagcagcg
      721 agcgctccaa cgccaaaagc cataaatcgc cgtgctgaat gtgcgcccat tcgtgggcca
      781 gcgccgcgcg gacgagcagc gcgttggcgg ggacggcagt ggaatgcggc aggacaatcc
      841 gcggcgccag cgcgccgacc gcaaccgccg tttccagccg ctcgctggtg cggactcgcg
      901 gcgagcgctg gccacgtccc gtgatgccgg ccagctctgc ggcaatccaa tctgccgccg
      961 gctgcgtgcg acccatcagc cgccgcatcc gccaccaacc gatcaatacc cagccgatcg
     1021 ccgagaaaac tcccgaaagc caaccgacaa ctgcgagctt gggcaggctc aaaggagggg
     1081 ccggcagcga cgcaggcgct cgcgcggcag gcgtcggaac gagcggctcg cctggttctt
     1141 ggatcaggac catggtcgcc ggcaaatcga gcggaggggc gatcgcttgc gtcgcgctag
     1201 gaatggcgcg cgccggtcgc cccaccacgt cttgcacccg caaacgcggc catgccggcc
     1261 aggcggtcac gactgcggcg caggcaatcg ccagccacgc tccccacgcc agtcgtacgc
     1321 gacccgccgg atcgcgaacg acccacctca gaaccacggc cgctgccaac agcaccgtcg
     1381 ccagccacgc aaaatcgacc agccaccagc caagcggcgc cagaagagcg ttcatttcag
     1441 gccctccgat ttgcgcttcg tgcgtgctgc atgccggcca tgtttggcct gcgcgactag
     1501 ttcctccagc cggcggattt cctcgtccga tggccgcttc gcctccagag cgtgaaccag
     1561 atattcatcc accgccccgt caaacacgcg gtcgaggaat ccgcgggcca gcgcacgaac
     1621 cgtcggtccc ttgcgaaccc tggcaaaata cagatagaac ttgccgctcg ctctgtggct
     1681 caccaggccc ttctgctcca tcacctgcag gaggctgagc acggtggtgt aggccagcga
     1741 ctcgccctgc tcgttgatgg ccgcggtgat ctcgcgaacc gtggcctcgc cggcctgcca
     1801 gaggaccttg agggcctgca attcgcgctc ggaagggacg atttccatcg gcatggcggc
     1861 tcaaagtcgg gactgcggcg atctactggt ctgccagtag ataggcccgt aatcctactg
     1921 gccagccagt agatgtcaag agcgaaaaca gtgcctactc agtgccagga cctcgaacag
     1981 ggtgggcagc gcacaccctg tgcatcgcga tcacccgatc tccagcccca gcaaatagtc
     2041 gtccagggcc ttcttgagcc tggcttcctc tgctaccgcg gcggtgattt gcttccgcag
     2101 gtcgtcgttc gagtcttcct gctcggtcag ctttgtcacg tatttgcggt acaactcggt
     2161 ttctttgcca agctgcgcca tattctcgcg aatccgcttt tgctcggcgt cgatcacggc
     2221 gatctgccgc tccatttctt gccgctgttg agccacctgc tggatggccg cctggcgggt
     2281 tacgacctcc tgcagggcct tcttgaccgc ttcgctaacg accggctgat tgaggtaata
     2341 ggcgatcgtg ccgctgtcga ggttcgtcat cgcgatctgc tcgctctgcg tgtactcctc
     2401 cttcacgtcg agcgtggccg gcttgccggg ctcggccttc aaggcgaagc gatactggct
     2461 gcgggttttc tcggcggggt ccttcggctc gaccaggttc cacggctgct ggatcgggta
     2521 ttcgaccagc acggtcttgt cgcgcttgcc tgagttcttg acggtgtacg acttcgtccg
     2581 ggcatatttg tggctggagt gcagcgtccc cttggcgatt cgcacggccg tcagccgccg
     2641 cgggtcgtcc ttctgcgtcg gggccacctc cgtatcgagg tccattgcat agctgatcag
     2701 ccgctcgctg ccggggggaa ggtccatgat ctgggcgtcg ccggcatagg cgttgtcgtc
     2761 gaagacggtg atcggccctt gcatcaggtg cagctcgctg ctgttcttca gcttcaggcc
     2821 attgagtggg tgcttgggat gcgtagcttc gttgtagatc gagaccttct cgccctggac
     2881 cgactcgttg acgatcggca gcatggccga tttgccgcgg gcgagtttga cgggcgtggc
     2941 gatgacgtag cgaaacattt cgcccacgtc gctggcctgg gcgacggatt gcacgccttg
     3001 ctggagttgg agagtgcggg ccttttcgtc ggctttggcc aggtagccaa agtcgcccgg
     3061 cctgccggct gcggctggtg cggggggcgc ggccggaacg acgccgcggc gggcgagcga
     3121 ctcgcgctgc cgattctgcg cctcgagcgc gtcgatcccc gcgcccttga actccgcctc
     3181 cttctt
//
LOCUS       DASUGB010000815         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_367408, whole genome shotgun sequence.
ACCESSION   DASUGB010000815 DASUGB010000000
VERSION     DASUGB010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367408"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VFV87_15505"
     CDS             <1..1489
                     /locus_tag="VFV87_15505"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5105226.1"
                     /translation="ARMAFQPASQTQQLAAVRQQLDALSRQKEQLERQLADAVPGFRA
                     KLIADEPPPHTRLAEQLPAKTAYIDFLQFRYVPPGATGLQPSSPRLKEIAERTVCYAA
                     FLLLAGNQAKRIDLGPAQPIDEAIDRWRREIVAGRAGTAGFELRNLLWQPIEEQLSGG
                     VETIYLVPDGRLSSVPWAALPGKARGTVLLEQFAIALVPHGQFLLQRLTPYQPAADLS
                     RVLAVGNPDFGASGDASRDNWPRLPGTQQELGEIAAFAGGRATVISGTDATTDRVLRE
                     LPRASLAHLATHGFFADAELRSFLQLDAETFAERDRGVGLRTSVVERNPLLLSGLALA
                     GANQSNGDEMPQAPSGILTAETIAGLPLDKLYLAVLSACDTGLGDVAGGEGVFGLQRA
                     FHQAGAQNVVASLWKVNDESTAALMRLFYYNLWEEKLPPLEALRRAQITLYRGPDQIN
                     ALATTTRGPNFNKVVKLVEGRAVRENPERSPPRVWAAFVLSGPGR"
     gene            complement(1554..1796)
                     /locus_tag="VFV87_15510"
                     /pseudo
     CDS             complement(1554..1796)
                     /locus_tag="VFV87_15510"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
BASE COUNT          390 a          632 c          614 g          371 t
ORIGIN      
        1 ggcgcggatg gcttttcagc cggcatcgca gacgcagcaa ctcgcggcgg ttcgccagca
       61 actggacgca cttagccggc agaaggaaca gctggagcgg caacttgccg atgcggtccc
      121 tggttttcgc gcaaaattga ttgccgatga gccgccgccc cacacgcggc ttgccgagca
      181 actcccagcc aagacggcgt acatcgattt tttgcagttt cggtatgttc cgcccggcgc
      241 gacgggactg caaccatcca gcccgcgact aaaagaaatc gcggaaagaa cggtctgcta
      301 tgcggccttt ctgcttctgg cgggcaatca ggcaaaacgc atcgatctgg ggcccgccca
      361 gccaatcgac gaagcaatcg accgctggcg gcgcgagatc gtcgcgggcc gcgcgggtac
      421 agcgggcttc gagctgcgca atctcctgtg gcagccgatc gaggagcagc taagcggtgg
      481 cgtggaaacg atctatctcg tgccggacgg ccgcctcagc agcgttcctt gggcggccct
      541 gccgggcaaa gcacgcggaa cggtgctgct cgagcaattc gcgatcgctc ttgtcccgca
      601 cgggcagttc ctgctgcagc ggctcacgcc ataccaacca gccgcggatt tgtcgcgcgt
      661 gttggccgtg ggtaatccgg actttggcgc cagcggcgat gcttcgcgcg acaactggcc
      721 tcgacttccc ggaacgcaac aggaactggg cgaaattgct gcgtttgcag gcggccgcgc
      781 gacggtgatc tctggcactg acgccacgac cgaccgcgtc ttgcgagagt tgcctcgggc
      841 gagcttggcg cacctcgcga cccacggctt ctttgccgat gccgagctgc gatcgttctt
      901 gcagcttgac gcggaaacat ttgccgagcg cgaccgcggc gtcggcctcc ggacatccgt
      961 cgtagagcgg aatccattgt tgctctctgg actcgctctg gccggcgcca atcaatccaa
     1021 cggcgacgaa atgccgcaag cgccaagcgg catcctgaca gccgaaacga tcgccgggtt
     1081 gccgctagac aagctgtacc tggcggtcct ttcggcctgc gatacgggct tgggcgacgt
     1141 ggccggcggg gaaggagtct tcggcctgca gcgggccttt caccaggccg gcgcccagaa
     1201 cgtcgttgcc agcctgtgga aggtcaacga tgaatcaacg gcagcgctga tgcggctctt
     1261 ctactacaac ctgtgggagg agaaactgcc gccgctggag gcgctgcgcc gcgcgcagat
     1321 cacgctgtat cgcggccccg atcaaatcaa cgctctggcc acgaccacgc gcggaccgaa
     1381 cttcaacaaa gtggtcaagc tcgtggaagg gcgcgcggtc cgcgagaacc ccgaacgcag
     1441 cccgccgcga gtttgggctg cgtttgtatt atcgggtccg gggcggtgag ttggataact
     1501 cacaataaac cggaccggag ccttcgagcg ctccgtagag agggtttggc aggttattcg
     1561 cgaagcttcg ccagcgtgct gttggcgacc gccaaagcct gttccaatcg ccgcacgtcg
     1621 gtcacgtccc agaacatgcc ttgcgtgccc accgtctttc cattggcatc ttgaacaggc
     1681 aacttcacga cgcggacgta catcacttcc tggttcgccg tcaggtgctc ttccatactg
     1741 ctgaatgttt cgcccgtggc gatcaccgcg gcatcgtcgt cgcgatattt tgcgacagct
     1801 ccggcggaaa caaatcaaag cgcccctcgg catccttgcg ccattagtaa tgggtcggga
     1861 ctgcgcacac aatatccatt acggtgggat tcatagcagc aagagatgca tgccaattgc
     1921 cgttagcccg gattattcac ggcccatgaa atgaaacagc ctccagacgc gtctaagaag
     1981 ttgagtaaca acgacttccc aaagcgc
//
LOCUS       DASUGB010000816         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1757063, whole genome shotgun sequence.
ACCESSION   DASUGB010000816 DASUGB010000000
VERSION     DASUGB010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757063"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFV87_15515"
     CDS             <1..973
                     /locus_tag="VFV87_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105227.1"
                     /translation="IAKAVKDGWTKFAHWWNHGPATTPIKITLLVIVAIAAIANGGWL
                     VPAAAVLGAVYLTYLGIRMLTNVVNKPAGSPFAEGHPPPLPHQAAWHITWEQHGRQML
                     CEKTAGDRIGELTGSMLAAAAIAAVLAVVMTAIGGETVSASTSQYAGPVWLWLATTAG
                     SWLVLGAGKFCERTSGEQVKRRFAMLVLGLFFGAAAWASSEFLMVKLHDGFVARSIAG
                     NSFARSMYDVSGDPRITAYLAYFGAIFLMIGWWKQVDPLRSSRLRIAPILLTILAAWI
                     VQPLMPFPQPWGFMLVAAISITSQLSAPWLSPAERTAAANRRGVTPT"
     gene            987..1898
                     /locus_tag="VFV87_15520"
     CDS             987..1898
                     /locus_tag="VFV87_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105228.1"
                     /translation="MLTIIPILALTTLAAVMLVAAWPLLCLPRLRPVFAGATGTSCIA
                     AGASLIALVMSGLLPGMTPTAVAQDEATTKSEAAAPSSTEGSGASESAPSPEPRAPNP
                     SSPDPVLEVPPTDEVIIPPGRPSWVEQDPQTDSSGTHTIPVSSGPFKRHIDALRGLDA
                     ELVDETDRYIADYLGSELAPAILSYDAATIRRELVKPEDIYEEKIISPTAGEMHQIHA
                     RIDIDPSFRDRLQQQWQKVRATSRLTQMGLFAGAGLLLIGSIFSYFRLDNATRGYYTG
                     RLQFLTAAAILAVIGGSVFAARWIHWL"
     gene            1967..>2241
                     /locus_tag="VFV87_15525"
     CDS             1967..>2241
                     /locus_tag="VFV87_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105229.1"
                     /translation="MSSTHDADGLLIERIRRGEDDAWDDLISRYEGRLLAFVESRLGR
                     RGPSEDIVQESFLGFLNSLPNYDGRRPLESYLFSICAYKLTDHLRREG"
BASE COUNT          369 a          796 c          685 g          391 t
ORIGIN      
        1 gatcgccaaa gcggtcaaag acggctggac gaagttcgcc cactggtgga accacggccc
       61 ggccacgacg ccgatcaaga tcacgctgct cgtcatcgtg gcgatcgccg ccatcgccaa
      121 tggcggctgg ctcgttccgg ccgcggcggt tctgggcgcg gtttacctca cgtacctcgg
      181 gattcgcatg ctgacgaatg tggtcaacaa gccagccgga tcaccgtttg ccgaaggcca
      241 tcctccgccg ctgccgcacc aggccgcctg gcatatcacc tgggagcagc acggccggca
      301 gatgctgtgt gagaagacgg ccggcgaccg gatcggcgaa ttgaccggtt cgatgctggc
      361 tgccgcggcg attgccgccg tgctagcggt cgtgatgacc gcgatcggcg gggagacggt
      421 cagcgcgtcc accagtcaat atgctggtcc ggtgtggctc tggctggcga cgaccgccgg
      481 ttcgtggctg gtgctgggag cgggcaagtt ctgcgagcgg accagcggcg agcaggtcaa
      541 gcgccgcttt gcgatgctcg tgctgggctt gttttttggc gctgcggcct gggccagcag
      601 cgaattcctg atggtcaagc tgcacgacgg attcgtcgcc cgcagcattg cgggcaattc
      661 gttcgcccgc agcatgtacg acgtgagcgg cgacccgcga atcaccgcct atctggctta
      721 cttcggcgcg atcttcctga tgatcggctg gtggaagcag gtcgatccgc tccgctcgtc
      781 gcggctgcga atcgccccga tcctgctcac gattctggcc gcctggatcg tccagccgct
      841 gatgccgttc ccgcagccct ggggctttat gctcgtggcc gccatttcga tcacctcgca
      901 actctccgct ccctggctga gcccagccga gcgaaccgcg gccgccaatc gccgcggcgt
      961 aacacccaca taaccaaatt cgtcccatgt tgacgatcat ccccattctc gccctgacga
     1021 cgctggcggc cgtcatgctc gtcgccgcct ggccgctgct ctgcctcccg cggctgcggc
     1081 cagtcttcgc cggcgcgacg ggcacaagct gcattgccgc cggcgcctcg ctcatcgccc
     1141 tggtcatgag cggcctgctg cctggcatga ctccgaccgc cgtcgcccaa gacgaagcga
     1201 caaccaaatc agaagcggcg gccccgtcct ccaccgaagg cagcggcgct tctgagtccg
     1261 ccccgagccc cgaaccccga gccccgaatc cctcctcccc cgaccccgtc ctcgaagttc
     1321 cccccaccga cgaagtcatc attccgccgg gccggccgag ttgggtcgag caggatccgc
     1381 agaccgacag ctccggcacg cacacgattc ccgtctcgtc cggtcccttc aagcggcaca
     1441 tcgacgccct ccgcggcctg gacgcggagt tggtggacga gaccgaccgc tacattgcgg
     1501 attacctcgg cagcgagctg gccccggcca tcctaagcta cgacgctgcc accatccgcc
     1561 gcgagctggt caagcccgag gacatctacg aagaaaaaat catttcgccc accgcgggcg
     1621 aaatgcacca gattcacgcc cggatcgaca ttgaccccag cttccgcgac cgactgcagc
     1681 agcaatggca aaaggtccga gccaccagcc ggctgacgca gatgggcctg tttgccgggg
     1741 cggggctctt gctgatcggt tcgattttca gctatttccg gctggataac gcgacgcgcg
     1801 gctattacac cggccggttg cagttcctga ccgccgccgc gatactggcc gtcatcggcg
     1861 gcagcgtctt cgccgcccgg tggattcact ggctgtaacc caaagccgcg accagtggtc
     1921 gcggttcccg cccgacaacc gcgaccactt gtcgcggctt tgaggcatgt cctctaccca
     1981 cgatgccgac ggcctcctga tcgagcggat tcgccgcggc gaggacgacg cgtgggacga
     2041 cctgatcagc cggtatgaag gccgcctgct cgccttcgtc gaaagccggc tgggccgccg
     2101 cgggccgagc gaggacatcg tccaggaatc gttcctgggc tttctgaaca gcctgccgaa
     2161 ttacgatggc cgccgcccgc tggagagcta cctgttctcg atctgcgcct acaagctgac
     2221 cgaccatctc cgccgcgaag g
//
LOCUS       DASUGB010000817         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_784758, whole genome shotgun sequence.
ACCESSION   DASUGB010000817 DASUGB010000000
VERSION     DASUGB010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784758"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VFV87_15530"
     CDS             <1..1934
                     /locus_tag="VFV87_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5105230.1"
                     /translation="ENPAAAYWKIIRDPAALMENTTHLFLAVRFNCNKCHDHPFERWT
                     QDQYYQLSQYFAQVTRKEDPEFAGKKVGGSAVEGAVPLVEVIYDGKSGDVKHDRTGEV
                     VPPKFPYEHADVNPNLPDRRQQLAQWVTSPANQYFAKSYVNRLWGYLFGAGIIEPIDD
                     IRAGNPPTNPELLDALTKDFIESGFDAQHILRTICKSRTYQLSVVTNKWNEDDTLNYS
                     HNVPRRLPAEVLYDAIYLATGAQEDLPGVPAGFRAAQLPDAGLSVPFLDDFGRPVRES
                     ACECERSSGMVLGPIMKLINGPTVADALAAPGSELNQLVATQPDDAKLIEEVFVRFLA
                     RKPTEQELKLGVEALRAAAGDQAQAAAALAEYEKQIPAKQAAWEASVGRPLVWTPLDP
                     NELKSAVGATLTKQEDKSVVATGTLAKDVYTFVAPTELKGITAIRLEAINDGSLAAGG
                     PGRAQNGNFVVSELKATIAPKSDLSQVEPLELQNASADFSQENWHVSAAIDNREDTGW
                     AISPQFGKAHEAIFETKVDAGHEGGSIVTITLSQQFPDGTHLLGKFRLSVTDAQRPIG
                     GAKLPEAVAAALAVPAAGRSAEQAATIAAHYRTLDAELARLTADAQKAAEQAKNARQI
                     GIQDLGWALINSPAFLFNR"
BASE COUNT          446 a          736 c          694 g          335 t
ORIGIN      
        1 tcgaaaaccc cgcggcagcg tactggaaaa tcatccgcga cccggcggcc ctgatggaga
       61 acacgacgca cctgttcctc gccgtgcggt tcaactgcaa caagtgccac gaccatccgt
      121 tcgagcgctg gacgcaggac cagtactacc agctttcgca gtacttcgcg caggtgacgc
      181 gcaaggaaga ccccgaattc gccggcaaga aggtcggcgg cagcgcggtc gaaggcgcgg
      241 tgccgctggt cgaggtgatc tacgacggca agagcggcga cgtgaagcac gaccggacgg
      301 gcgaggtcgt gcctccgaaa ttcccctacg agcacgcgga cgtcaatccc aacctccccg
      361 accgccgcca gcagctcgcc cagtgggtca cttcgccagc gaaccagtac ttcgccaaaa
      421 gctacgtcaa ccgcctctgg gggtatctct tcggcgccgg catcatcgag ccgatcgacg
      481 acatccgtgc cggcaatccg ccgacgaatc ctgaattgct cgacgccctg acgaaagatt
      541 tcatcgagag cggcttcgat gcccagcaca tcctccggac gatctgcaag tcgcggacgt
      601 accagctttc ggtcgtcacc aacaagtgga acgaagacga tacgctcaac tactcgcaca
      661 acgtaccccg ccgtttgccc gcggaagtgc tgtacgacgc gatctatctg gccaccggcg
      721 cccaggaaga cctgccgggc gtgcccgccg gattccgcgc ggctcagttg ccggatgccg
      781 gcctgtcggt tccattcctg gacgattttg gccgcccggt ccgcgaatcg gcctgcgaat
      841 gcgagcgctc cagcggcatg gtgctgggac cgatcatgaa gctgatcaac ggaccgaccg
      901 tggccgatgc cctggcggcg cccggcagcg agttgaacca gctcgtcgcc acgcagccgg
      961 acgatgcgaa gctgatcgag gaggtcttcg tccgcttcct ggcacgcaaa ccgaccgagc
     1021 aggagctaaa gctcggcgtc gaggccctgc gggccgctgc gggcgaccag gcccaagccg
     1081 ccgccgcact ggcggaatac gagaagcaaa tcccggcgaa gcaagccgcg tgggaagcct
     1141 ccgtcggccg accgctggtc tggacgccac tcgatccgaa cgagttgaaa tcagccgtag
     1201 gcgcgacgct gacgaagcag gaagacaagt cggtcgtggc gacgggaacg ctggccaagg
     1261 acgtctatac tttcgtggcg ccgacggagc tcaaaggcat cacggcgatc cgcttggagg
     1321 ccatcaatga tgggtcgctg gccgccggcg gcccgggccg cgctcagaac ggcaacttcg
     1381 tcgtcagcga gctgaaggcg acgatcgcgc cgaagagcga cctctcccag gtcgagccgc
     1441 tggagctgca gaacgcctcc gccgatttca gccaggaaaa ctggcatgtt tcggcggcga
     1501 tcgacaatcg cgaggacacc ggctgggcca tttcgccgca gttcggcaag gcgcacgagg
     1561 cgatcttcga gacgaaggtt gatgcgggcc acgaaggcgg ctcgatcgtc acgatcacac
     1621 tgtcgcagca attccccgat ggcactcacc tgctggggaa attccggctg agcgtgaccg
     1681 atgcccagcg gcccatcggc ggcgcgaagc tccccgaagc cgtcgccgca gccctggccg
     1741 tccccgccgc cgggcgttct gcggaacagg ccgcaacgat cgccgcgcac tatcgcacgc
     1801 tcgatgccga gctggcccgc ctcaccgccg atgcccagaa agccgccgag caggcgaaaa
     1861 atgcccggca gatcggcatt caggacctcg gctgggcgct gattaacagc ccggcgtttc
     1921 tcttcaatag gtagcgccga caccaacccg aagcgtcagc gagggagaca caccaacccg
     1981 acccgtcagc gaaggactcc ctcgcttgcg cttcgggctg gtgtgcctct aggcaggctg
     2041 acgagcgtgc gttacctttg gccgctgatt cgcgtggccg tctggtcagt ggcctctggc
     2101 acgatctgtt cttcaataca ctcagccaag gacggcggca tgccagagca gcaatcggag
     2161 cgaagggtgg ttgacaagac ggccagcgca gatacaaatt gacatgaagg t
//
LOCUS       DASUGB010000818         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1339774, whole genome shotgun sequence.
ACCESSION   DASUGB010000818 DASUGB010000000
VERSION     DASUGB010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339774"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      152..405
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            592..2883
                     /locus_tag="VFV87_15535"
     CDS             592..2883
                     /locus_tag="VFV87_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5105231.1"
                     /translation="MLPSILQSVGITGLVLIVLSLKLLFLAWLTLRFIPNNYVGVVEK
                     LWSAKGSVPEGHILALDDEAGFQADLLRGGIHFGYWRWQYRVHRCPLVTVPQGKIGYV
                     YSRDGQPLPPNQTLGRTIDCNHFQDARAFLVGAVPGEATACLPQASSATASSTTGQRG
                     RQLAILREGVYAINPALFVVLTEDAVYTLRHLLSRHELASVEEWQEELQEIDGFEPVI
                     IGEPVEAIDPLHPELRTKVDSIGIITVHDGPSLAPGEIIAPAVGCDVGDKHYHSNFQD
                     PQRFLDGGGRRGRQYQPLTDGTYFINRWFATVELIPKTVVPIGYVGVVVSYYGQAGDD
                     LSGIAFRHGERVTEGRRGVWERPYGPGKYAFNTYAGNIVLVPTTNFVLHWVTGRSETH
                     RYDDSLRSIDLVTRDAYEPRLPLSVVVHIDYQRAPNVIQRFGDVKKLITQTLDPLLSA
                     YFRDIAHRKTMLELLHERDAIQAESRDELRRRFREFDIECVDVLIGKPDTDEKDGKIE
                     SLLEQLRIRQLSIEQLETYERQREASDKRRALAEAQAQADMQTSLTNARVKAEIAESE
                     GEADLKRAKKQAEKQIVMSQAELAQSRHHAEQTLVLAEADSQQAILAGKGEAQRIVQM
                     GLADAAVLMQKVASYGDPRLFALSQVGTQLAKSTQPLVPERVFIAGGPSGETGTRSVS
                     EGGNGDGHGLNGSAPTIATAGIVGLLLQSLLADRTAFAGGNGPETAALQELASRMTRE
                     TLANLHQESPTAGLPGMDNASAG"
     gene            3077..3484
                     /locus_tag="VFV87_15540"
     CDS             3077..3484
                     /locus_tag="VFV87_15540"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5105232.1"
                     /translation="MSQFHQFIEKIQADGKIGDQEVQQIREEIAADGQLDIADVKLLV
                     ELYCGAKERSPQFDDLFFEVLETVFLSDGRISSSEEFYLLKMLYSDREITEREREFLR
                     RVRKQLPERAASFESLFETAINAPNTNWCVGGR"
     gene            complement(3647..>4293)
                     /locus_tag="VFV87_15545"
     CDS             complement(3647..>4293)
                     /locus_tag="VFV87_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX5105233.1"
                     /translation="RLWQLQVGQCIEVWGPLGNGFGASHHSPLTTHHSPSAKPPPVPT
                     EHLILVAGGIGFTPFLALSQEALHKKSYGDPPRAASRALHITYCYGARSADYFADLAA
                     FEQLGVEIRLTTDDGSRGRHGLVTELLPDVLAGHHGRPHIVCCGPEKMMEAVAAIARE
                     ANVPCEVSLETPMACGIGICFTCVAKVRQADGDWDWKRTCVEGPVFDAEEIWWP"
BASE COUNT          794 a         1387 c         1370 g          742 t
ORIGIN      
        1 acctcagggc tgaggcacgc ctagtttgcg cggaaattcg gccccagcaa cctcgccgta
       61 agatgctgcg gcgattgtgt ttgcgccggg ctggtcagtt ggcgtccggt ttgcatggcg
      121 caagactcgc ggtcgccaga cgatataatt tctatcgtgc aagcctaatc ctgcgccatg
      181 tgtgcaggag cgggggaccc aatgcgttgg cggcgttcgt aggacggctt ttccaagctg
      241 cccggcacgg gttgaaaacc cgtgctacga actcccaact ccggggcgca ggttgaagcg
      301 gcccagcgag ccgcgaggca ggctggaagc ctgctccact tcgaccgggg cacctttcaa
      361 gccctagccc gtcagctaac ctcgtcggcg gatcggcggc gcgtgctgct ggtccacaag
      421 ttgaaagggc cttctcgtcc gccgctcgga cggtcaagcc cggtttcgaa tcgtagcccg
      481 tagcgccagc gagggtaatc cgcgcggcgc tgccaagatc gtcgcaccga cgcgcgggtc
      541 agcctccatt ccctcgctcg cgctacgggc tactgttcca ggaggcgttc catgctccct
      601 tccattctcc aatcggtcgg catcacgggc ctggtgctga tcgtgctctc gctcaagctc
      661 ctgttcctgg cctggctgac gttgcggttc attccgaaca actacgtcgg cgtggtcgaa
      721 aagctttggt ctgccaaagg ttccgtcccc gaaggccaca tcctcgccct cgatgacgag
      781 gcgggttttc aagcggatct gctgcgcggc ggcatccatt tcggttattg gcggtggcaa
      841 taccgcgtgc atcgctgccc gctggtcacc gtgccgcagg gaaaaatcgg ctatgtctat
      901 tcccgcgacg gccagccgct gccgccgaac cagacgctgg gccgcaccat cgattgcaac
      961 cacttccagg acgcccgcgc gtttctcgta ggagctgtgc cgggagaagc cactgcttgc
     1021 ctgccccagg caagcagtgc cacagcaagc agtaccacag gccagcgcgg ccgccagctc
     1081 gcgatcctcc gcgaaggcgt ctatgcaatt aacccggctc tgttcgtcgt cctcaccgag
     1141 gacgccgtct atacgctccg ccatctgctg tcgcggcacg aactggcgtc ggtggaagaa
     1201 tggcaagaag aattgcaaga gatcgacggc tttgagccgg tgatcatcgg cgagccggtc
     1261 gaagcgatcg acccgctcca tcccgagctg cggacgaaag tcgatagcat cggcatcatc
     1321 acggtccacg acggcccgtc gctcgctccg ggcgagatca tcgctccggc cgtcggctgc
     1381 gatgtgggcg acaagcacta ccacagcaac ttccaggacc cgcagcggtt cctcgatggc
     1441 ggcggccgcc gcggccggca gtatcagccg ctgaccgacg gcacctattt catcaaccgc
     1501 tggttcgcca cggtcgaatt gattcccaag acggtcgtac cgatcggcta tgtcggcgtg
     1561 gtcgtcagtt attacggtca ggcgggcgat gacctttccg gcatcgcgtt ccgccacggc
     1621 gagcgcgtca ccgaggggcg gcgcggcgtt tgggaacggc cgtatggccc gggcaaatac
     1681 gccttcaaca cctacgccgg caatatcgtg ctggtgccga cgacgaactt cgtgctgcac
     1741 tgggtcaccg gccgcagcga gacgcaccgc tacgacgatt cgctgcggtc gatcgatctg
     1801 gtcacgcgcg acgcctacga gccgcgcctg ccgctcagcg tggtcgtgca tatcgactac
     1861 cagcgggcgc cgaacgtgat ccagcgtttc ggcgacgtga aaaagctgat cacgcagacg
     1921 ctcgacccgc tgctgagcgc ctatttccgc gacatcgccc acaggaagac gatgctggaa
     1981 ttgctgcacg agcgtgacgc cattcaggcg gaatcccgcg acgagctgcg ccgccgattt
     2041 cgcgagttcg acatcgagtg tgtcgatgtg ctcatcggca aacctgatac cgacgagaag
     2101 gacggcaaga tcgagtcgct cctggagcag cttcgcatcc gccagctctc gatcgagcag
     2161 ctcgaaacct atgagcgaca acgcgaggcg tccgacaaac gccgcgctct ggccgaggcg
     2221 caggcccagg ccgacatgca gaccagcctg accaacgccc gcgtgaaggc cgagatcgcc
     2281 gaaagcgaag gcgaagccga cctgaagcgg gccaagaaac aggccgaaaa gcagatcgtc
     2341 atgtctcagg ccgagttggc ccagtcccgc caccacgccg agcagacgct cgtcctcgcc
     2401 gaggccgata gccagcaagc cattctggct ggcaaaggcg aggcccagcg gatcgtgcaa
     2461 atggggctgg ctgacgctgc cgtgctgatg caaaaggtcg ccagctacgg cgacccgcgc
     2521 ttgttcgccc tgtcgcaagt cggcacgcag cttgcgaaga gcacgcagcc gctggtgccc
     2581 gagcgggtct ttatcgccgg aggcccgagc ggcgagacag gaacccgcag cgtaagcgag
     2641 ggagggaatg gcgatggcca cggtttgaac ggctcggctc ccacgatcgc cactgcaggc
     2701 attgtggggc tcttgctgca gtcgctgctg gcggatcgaa cggcgtttgc gggcggcaac
     2761 ggccccgaaa cggcggcgct ccaggagttg gccagccgca tgacgcgcga aacgctcgcc
     2821 aacctccacc aggagagtcc gactgccggc ctgccaggta tggacaacgc atcggccggc
     2881 taaatgctca ttccaacggg gcggcggtgg attccctggc tggttggaac gaagattccg
     2941 gctgtgcggc ccgcggcgcg tgctacggtt gaaggagcgt cgtcgcgccg cggtttcgcg
     3001 ccggcggacg ccgcggaacc gcgaccagtg gtcgcggctt tgtgtcattg ttgccccaaa
     3061 cacgggatct caaaccatga gccagttcca tcagttcatc gagaaaatcc aggctgacgg
     3121 caagatcggt gaccaggaag tgcagcaaat ccgcgaggaa atcgcggctg acggtcagct
     3181 cgacatcgcc gacgtgaagc tgctcgtgga gttatattgc ggcgcgaagg agcgctcgcc
     3241 ccaattcgac gacttgttct tcgaagtcct ggaaacggtc tttctcagcg atggccggat
     3301 ctcgtccagc gaagagttct acctgctcaa gatgctctat tccgaccgcg aaatcaccga
     3361 gcgcgagcgg gaattccttc gccgcgtgcg gaagcagttg cccgagcggg ccgcgagctt
     3421 cgaatcgctg ttcgaaacgg cgatcaatgc ccccaatacg aactggtgcg tcggcggccg
     3481 gtaggcatcg gcgctgtagg gaacgccctc cgtggcgttc cgaggggttt cccaacccgt
     3541 ccgagtctcg atcccggata tcgtttaacg cccagccgca ggcgtagctg gacacggtct
     3601 ccacctctga aagccgctac gccttcggct gcgcgttaaa cgagcgctac ggccaccaga
     3661 tctcctcggc gtcgaagacc gggccttcga cgcaggtgcg cttccagtcc cagtcgccat
     3721 ccgcctggcg gaccttggcg acgcaggtga agcaaatccc gatgccgcag gccatcggcg
     3781 tttcgagcga gacttcgcaa gggacgttcg cctcgcgggc gatcgcggcc accgcctcca
     3841 tcatcttttc ggggccgcag cagacgatgt gcggccgccc gtgatgcccg gctagaacat
     3901 ccggcagcaa ttcggtcacc aggccatggc gcccgcggct gccgtcgtcg gtcgtcagac
     3961 gaatctcgac gccgagctgc tcgaaagccg cgagatcggc gaaatagtcc gccgagcggg
     4021 cgccgtagca atacgtgatg tgcagcgccc gcgatgcggc gcgaggcgga tcgccgtacg
     4081 acttcttgtg cagcgcctct tgcgacaagg cgagaaacgg agtaaagccg atgcctccgg
     4141 cgaccaggat gaggtgctcg gtgggaacgg gtggcggctt ggcggaaggt gagtggtgag
     4201 tcgtgagtgg tgagtggtgg ctcgcgccga atccgttgcc aagcggtccc caaacctcga
     4261 tgcactgtcc cacctgcagt tgccacaggc ggc
//
LOCUS       DASUGB010000819         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_229864, whole genome shotgun sequence.
ACCESSION   DASUGB010000819 DASUGB010000000
VERSION     DASUGB010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229864"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /locus_tag="VFV87_15550"
     CDS             complement(<1..2032)
                     /locus_tag="VFV87_15550"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5105234.1"
                     /translation="MADPISTCLIFIPALPLAAAVAVAILGARLLKEHSHLPVVLALL
                     GSFFFSIVLWREVLAAQKANPAGGFEQVVPLWTWAEVPEAYDLKANPPAVTDSEAGLR
                     NFRIDITLRADALTAMMLSMVTFVSALVAVFASGYMHGDRAYWRFFALIGLFVFSMTM
                     LVSVSNFVLLFVFWEAVGVCSYLLIGFWYEKPAAAAAGKKAFLVNRVGDFAFTIAMFL
                     IWTTYGTLNYHDTYKDGSLVPADQPVLDAIENTPSADGRFIRGVLGKSRIETGDFAAG
                     GIATAICLLLLLGACGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVYMVARCT
                     PLFAVSPTAQLVVAGIGGFTALMAALIALTQFDLKRVLAYSTISQLGYMFLALGAGTM
                     AGVSGGMYHLFTHAFFKALLFLGAGSVMHAMGGVIDMRQFGGLKRLMPHTYWTFLVGC
                     LALSGVFPLAGFWSKDTIFSAVHEKVHAIEHEIEHRAEPKPGQEPGVRGQESAASPLT
                     SWTDAQLATSRRAYQWLYWGAVFTAFLTAFYTFRAFFLTFHGPERIPAEAGHHAHESP
                     AVMLMPLAALAICAASIGLVVVLLSDPTNWGANDLTAFLTHTPSLAAGTIAATATEPT
                     FHLSIAGLSTVVAVAGILLAAYLYLGERSEVAYVQRIFDLQGLDRLTDPQWVV"
     gene            complement(2115..2558)
                     /gene="nuoK"
                     /locus_tag="VFV87_15555"
     CDS             complement(2115..2558)
                     /gene="nuoK"
                     /locus_tag="VFV87_15555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5105235.1"
                     /translation="MNETALLYNFLAVGGVLFSLGLVGFLVRRNMIVMFLCAEMMLQG
                     VSISLVGFGRYHNDWGGQMLVVFIITVAACEAGLAMALILMLAQRAGKLDSTTWQDLR
                     EEGLPAYVDHEVPEESAADQAWPHLTPAGIEPPIDRDALAHRSRV"
     gene            complement(2555..3385)
                     /locus_tag="VFV87_15560"
     CDS             complement(2555..3385)
                     /locus_tag="VFV87_15560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5105236.1"
                     /translation="MNPLLLAAADKAAGLTARDVFACPTFWALVCGVLGLWFVLPARF
                     RFGNAIGSVLLAIAGGLFAFDLPLLGPRLDQGVFWLLAGVTLAASVCTISSRSPVYCA
                     IWFALSLLGTAGLYLFQGAQFLGVATIVVYAGAIVVTFLFVIMLAQPEGHTMYDRISW
                     GWFPKVFGVVAAGTLVGILTFMLGGLKDASTRPVSADAVAAADQRLQADAGILSDHHM
                     ANLGRHLFAEHLVSVEVAGTLLLAALVGAIAIAAYGKQASGGRQPPEGFAARIEEALR
                     "
     gene            complement(3382..>3821)
                     /locus_tag="VFV87_15565"
     CDS             complement(3382..>3821)
                     /locus_tag="VFV87_15565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX5105237.1"
                     /translation="LARSLRGDVVTVSYPDEEPQIGNPQIYRGVHRLNKDAAGRVKCV
                     ACFLCATACPAHCIDIIAEPSPWPDREKYPQSFSIDELRCIYCGMCEEACPVDAIELT
                     SLYNLTGRSREEMIFDKEKLLSVFDMTKDKEPMQSPAMGGTAT"
BASE COUNT          773 a         1177 c         1286 g          585 t
ORIGIN      
        1 gcacgaccca ttgcgggtcg gtcagccggt cgaggccttg caggtcgaag attctctgga
       61 cgtacgcaac ttcactccgc tcgccaagat acagatacgc ggccagcaag attccggcca
      121 cggccacgac ggtgctgagg ccggcgatgc tgaggtgaaa cgtgggttcg gtcgccgtgg
      181 ctgcgatcgt tcccgcggcc agcgaaggcg tgtgcgtcag gaaggcggtc aaatcgttcg
      241 cgccccagtt cgtcgggtct gaaagcaaaa cgacaaccaa tccgatgctt gcggcgcaga
      301 tggccagcgc cgccagcggc atgagcatga ccgccggcga ttcgtgcgcg tggtggccgg
      361 cttcggcagg aatgcgttcc gggccgtgga atgtgaggaa aaaggcccgg aaggtgtaaa
      421 aggcggtcag aaatgccgta aacacggcac cccaatagag ccactgatac gcacgccggc
      481 tggtggcgag ctgtgcgtcg gtccacgaag tgagcggact cgcggctgac tcctgacccc
      541 tgactcctgg ctcctgacca ggcttaggct ccgcccggtg ctcgatctcg tgctcgatgg
      601 catgaacctt ctcatggacg gcggagaaaa tcgtgtcttt gctccagaag ccggccagcg
      661 ggaagacgcc ggacagcgcg aggcagccga cgaggaaggt ccagtacgta tgcggcatca
      721 ggcgcttcag gccgccaaac tgccgcatgt cgatgacacc gcccatcgcg tgcatcacgc
      781 tgccggcgcc gaggaacagg agggccttga agaaagcgtg cgtgaagagg tggtacatgc
      841 cgccgctgac gccggccatc gtccccgcgc cgagggccag aaacatgtag ccgagctggc
      901 tgatcgtgga ataggccaac acgcgcttca ggtcgaattg cgtgagcgca atcagcgcag
      961 ccatgagtgc cgtaaagcca ccgatgccag ccacgacgag ctgcgccgtc gggctgacgg
     1021 caaacagcgg cgtgcagcgg gcgaccatat agacgccggc agtgaccatc gtcgccgcgt
     1081 ggatcagcgc gctaacgggc gtggggcctt ccatcgcgtc gggaagccag acgtgcagcg
     1141 ggaactgggc gcttttgcca catgccccaa gcaggagcaa caggcagatc gccgtcgcga
     1201 tgccgccggc ggcgaagtcg cccgtttcga tgcggctctt gcccaggacg ccgcggatga
     1261 agcggccgtc ggctgagggc gtgttttcaa tggcgtcgag gacgggctgg tcggcaggga
     1321 ccaggctgcc atccttatac gtatcgtggt agttcagcgt gccataggtc gtccagatca
     1381 ggaacatggc gattgtaaag gcgaaatcgc ccacgcggtt gacgagaaag gccttcttcc
     1441 ccgccgcggc ggctgcgggc ttctcgtacc agaagccaat cagcagatag ctgcagacgc
     1501 cgaccgcttc ccaaaagacg aacagcagca cgaaattgct gacgctgacc agcatcgtca
     1561 tcgagaagac gaacagaccg atcagagcaa agaatcgcca atacgcgcgg tcgccgtgca
     1621 tgtagcccga ggcgaagacg gccacgagcg ccgaaacgaa cgtgaccatc gagagcatca
     1681 tcgcggtcag ggcgtcggcc cggagcgtga tgtcgatgcg gaagtttcgc aggcccgctt
     1741 cggagtcggt gacggcgggc ggattggctt tcagatcgta tgcctcgggc acttccgccc
     1801 aggtccagag cgggaccacc tgctcaaagc cgccggcggg attggccttt tgagccgcga
     1861 gcacttcccg ccacagaacg atgctgaaga agaacgagcc gagcagggcc agcacgaccg
     1921 gcaagtggct gtgctccttc agcagccgcg cgccgagaat cgccaccgcc acggctgccg
     1981 ccagcggcag agccggaatg aagatcaggc aggtggaaat cgggtcggcc attgggagga
     2041 tcgtagccgc gttcgccaga acgcgggttg tcgcgggttt tcccgcactc tggcgagtgc
     2101 ggctacggtg gtccttaaac gcggctgcga tgggccaggg cgtcgcggtc gatcggcggc
     2161 tcgatgccgg cgggcgtgag gtgcggccag gcctggtcgg cagccgattc ctcagggact
     2221 tcgtgatcga cgtaggccgg cagcccctcc tcgcggagat cctgccaggt ggtggaatcg
     2281 agtttgccgg cccgctgggc gagcatcagg atcagggcca tcgccagtcc cgcttcgcag
     2341 gcagcgacgg tgatgatgaa cacgaccagc atctggccgc cccaatcgtt gtgatagcgg
     2401 ccaaagccga cgaggctgat ggaaacgccc tgcagcatca tctcggcaca gaggaacatg
     2461 acgatcatgt tccgccgcac gaggaagccg acgagcccca gggagaacag cacgccgccg
     2521 acggccagga agttatagag gagagccgtc tcgttcatcg gagggcctcc tcgatccgcg
     2581 cggcgaagcc ctcagggggc tgacgccccc cgctcgcctg cttgccatag gcggcgatgg
     2641 cgatcgcacc gaccagcgcc gccaacagca gcgtgccggc gacttcgacg ctcaccaggt
     2701 gttcggcaaa caaatgccgg cccaggtttg ccatgtgatg atcactgaga atgcccgcat
     2761 ctgcctgaag tcgctggtcg gccgcggcca ctgcatctgc ggagaccggg cgggtagaag
     2821 cgtctttcag cccgcccagc atgaacgtca ggatgccgac aagtgtgccg gcggcgacga
     2881 cgccgaagac cttgggaaac cagccccagc tgatccggtc gtacatcgtg tgcccttccg
     2941 gctgggcgag catgatcacg aacaggaacg tgacgacgat cgcgccggca tagacgacga
     3001 tcgttgcgac gccgaggaac tgggcgccct ggaacaggta gaggccagcc gtaccgagca
     3061 gcgacagggc gaaccagatc gcgcaataga ccgggctgcg ggagctgatc gtgcagactg
     3121 acgcggcaag ggttacaccg gccagcagcc aaaacacacc ctgatcgagc cgcgggccca
     3181 acagcggcag gtcgaacgca aacaacccgc cggctatcgc gagaaggaca ctgccgatgg
     3241 cgttgccgaa ccggaatcgt gcggggagga cgaaccacag gcccagcacg ccgcagacca
     3301 gcgcccagaa cgtcgggcac gcgaacacgt cccgtgcggt caacccggcg gctttgtcgg
     3361 cggcagcgag caggagcgga ttcacgtggc cgtccccccc atcgccggcg actgcatcgg
     3421 ctctttgtcc ttcgtcatat cgaaaaccga taacagcttt tccttgtcga agatcatctc
     3481 ctcgcggctg cggccggtga gattgtaaag gctggtcagc tcgatggcat cgacggggca
     3541 ggcttcctcg cacatgccac aatagatgca ccgcaattcg tcgatcgaga agctctgcgg
     3601 atatttctcg cgatcgggcc atgggctggg ctcggcgatg atatcgatgc aatgagccgg
     3661 gcaggcggtc gcacagagga aacaggccac gcacttcacg cggccggcgg cgtctttgtt
     3721 caggcggtgg acgccgcggt agatctgcgg gttgccgatt tggggctctt cgtcgggata
     3781 ggaaacggtg acgacgtcgc cgcggagcga gcgggcgagg t
//
LOCUS       DASUGB010000820         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_645038, whole genome shotgun sequence.
ACCESSION   DASUGB010000820 DASUGB010000000
VERSION     DASUGB010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645038"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFV87_15570"
     CDS             <1..371
                     /locus_tag="VFV87_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5105238.1"
                     /translation="PPTTPETVNLINRQTLAQMKPGAVLVNTARGAVVDADALCEALD
                     RGQLFGAALDVFKIEPLPTTSPLLKYDSVVLCTHMGGMDHDSAIATSSLAAQCIVDLY
                     HGRWPEACAVNRELRERWKW"
     gene            complement(474..806)
                     /locus_tag="VFV87_15575"
     CDS             complement(474..806)
                     /locus_tag="VFV87_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5105239.1"
                     /translation="MSLTMTEVAAKEVGNYKTQVQAEPESFLRVRITAGGCSGHSYSL
                     TLDKNYDEHKDSKFNFHGQDIVVDKKSELYLEGATLHFYEGLEQRGFHLDIPSAKKTC
                     GCGSSFQF"
     gene            complement(947..>2019)
                     /locus_tag="VFV87_15580"
     CDS             complement(947..>2019)
                     /locus_tag="VFV87_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5105240.1"
                     /translation="ARESIAAAIGAEAREIVFTSGATESNNLAIRGLAERQRRRGNHL
                     VSVTTEHKAVLDPLRRLSRRGFEVTLLEVEQHGSPRAGWLDPQRVAEAIQEDTLLVSV
                     MLANNEIGILQPIAEIAAICQSRGVPLHCDATQAVGKVPVDVRQLGIDLLSFSGHKIY
                     GPKGVGALYVRRGGPRLEPLIDGGGQENGLRSGTLNVPGIVGFAKALELCVAEFPAEM
                     LRLAGLRHRLWEGLRQAVPDVVLNGPEFEAVPSSGPDGVGGLLCRLPANLNCSFPFVN
                     GEALMMSMKTIAVSSGSACTSANPEPSHVLAALGLGEDLTRASLRFGLGRFNTEEEID
                     YAVAAVAENVHRLRQLSSIASP"
BASE COUNT          339 a          640 c          670 g          370 t
ORIGIN      
        1 atccccccac aacgccagag acggtcaacc tgatcaatcg ccagacgttg gcccagatga
       61 agccgggggc ggtgctggtg aatacggcgc gcggggcggt ggtcgatgca gatgcgctct
      121 gcgaggcgct cgaccgcggt cagctattcg gagcggcgct cgatgtcttc aagatcgagc
      181 cgctgccgac gacgagcccg ctgctgaagt acgacagcgt cgtgctctgc acgcacatgg
      241 ggggcatgga ccacgattcg gccatcgcca ccagcagcct ggcggcgcag tgcatcgtcg
      301 atctctacca cggccgctgg cccgaagcgt gcgcggtcaa ccgggagctg cgcgagcggt
      361 ggaagtggta ggtgccttta gccgggagca atcttgctcc cggagcgggc gtgttggtgt
      421 ggcggcgcga actcgcagtt ctccgggacc aggtttggtc ccggctaaga agcctaaaac
      481 tgaaacgagc tgccgcagcc gcaggtcttc ttggcggagg ggatgtcgag gtggaagccg
      541 cgctgctcga gcccttcgta gaagtggagc gtcgcccctt cgaggtacaa ctcgctcttc
      601 ttatcgacga caatgtcctg gccgtggaag ttgaacttgc tgtccttgtg ctcgtcgtaa
      661 ttcttgtcga gggtcagcga gtagctgtgc ccgctgcagc cgccggcggt gattcgcacg
      721 cggaggaagc tctcgggctc ggcctgcacc tgggttttgt aattgccgac ttctttggcg
      781 gcgacttcgg tcatcgtaag cgacatctgg cattctctcc tgtgaaattg cgattttcgc
      841 aatcgctcat gtcaaaagta gtatatcgcc cgcaggctgc ctggaaaagt gaatcggatc
      901 ggctggcgcg ggcagcttgt aagtgaaggc ccgcaggcga gttacgctac gggctagcga
      961 tgctgctgag ctggcgaagg cggtggacat tttcagcgac ggcggcgacc gcgtagtcga
     1021 tttcctcctc ggtattgaac cgccccaggc caaaccgcag gctggcccgc gtgaggtcct
     1081 cgcccaggcc taatgccgcc agcacgtggc tcggctccgg gttggcactg gtgcaggcgc
     1141 tgccgctgga gacggcgatc gtcttcatgc tcatcatcag ggcttcgccg ttgacgaagg
     1201 ggaatgagca gttgaggttg gcgggaaggc ggcaaagtag gcccccgact ccgtcggggc
     1261 cagaacttgg aaccgcctcg aattcgggtc cgttcagcac gacgtcagga acagcttggc
     1321 gcaatccctc ccacaaccgg tgccgaagcc cggccaatcg cagcatctcc gccgggaact
     1381 cggcaacaca aagctccagg gctttggcaa agcccacaat ccccggcacg ttcagcgttc
     1441 cgctgcggag gccgttctcc tgcccgccgc cgtcgatcag cggctccagg cgcggcccgc
     1501 cgcggcggac gtagagcgcg ccgactccct tggggccgta aatcttgtgg cccgaaaagc
     1561 tcagcagatc aatgccgagt tgccgcacat cgaccgggac cttgccgacg gcctgcgtcg
     1621 cgtcgcaatg cagtggcaca ccgcggcttt ggcagatcgc ggcgatttcg gcaatcggct
     1681 ggaggatgcc gatctcgttg ttggccagca tgacgctgac cagcagcgta tcttcctgga
     1741 tcgcctcggc cacgcgctgc ggatcgagcc agccggcccg cgggctgccg tgctgctcga
     1801 cttccagcag agtcacctcg aatccgcggc gcgacagacg gcgcagcggg tcgagcacgg
     1861 ccttgtgctc cgtggtgacg ctgacgaggt gattgccgcg ccgccgctgc cgctcggcca
     1921 ggccgcggat cgccaggttg ttgctctcgg tcgccccgct ggtgaagaca atctcccgcg
     1981 cctccgcgcc gattgccgcc gcgatcgact cgcgggccg
//
LOCUS       DASUGB010000821         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1479076, whole genome shotgun sequence.
ACCESSION   DASUGB010000821 DASUGB010000000
VERSION     DASUGB010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479076"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VFV87_15585"
     CDS             complement(<1..1728)
                     /locus_tag="VFV87_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5105241.1"
                     /translation="MPFVVAQLESLATWMVNPWLFVAGAAAVSLPIIIHLLNKRKFKI
                     VDWAAMEFLLDADKKNRRRIRLENLLLLLLRCLAVLLIALLLARPFLPTSVTAGLINA
                     AQFERIVLLDDSLSMQARLGNLSAWDSAQKRLIDLTGGLAQDQSADNSLTLFLTSRPN
                     QPLFSAAHLSQDSIDEINVTIERLEAGDRPANLDAALKEVESYLSSQPGNVNRVVYLL
                     TDLRRRDWKESDTAPEAPVKVLGWLSKLVQGCFVIDAGDDEDRNLTITEVRPEGTLVQ
                     GVASRFDVGVKNHGATDARDLRVKFSAGDALPVETTIDRLGPGEETSVRFNFTFSGGE
                     EPTEDARRQLPPRQVRVELLTGRQGEDDRLQADSVAFFPARLVRGIPVLIIDGDPSAD
                     FGKAESFYLRRNLAPPGPTASGVAVQIITENELEAADLGKYQVIFLCNNYRLGDKTAE
                     NIERLRKWVENGGGLVIMPGDQIDEHFYSEQYYKDGAGLSPVRLTNILGDETERTWVN
                     LKVENANHEVFVEFAGQNNPLLDSVKVFRWWGSAVKPEQLGKEVSVLARFSDADDSLA
                     VAEKPLGKGR"
     gene            complement(1738..>2453)
                     /locus_tag="VFV87_15590"
     CDS             complement(1738..>2453)
                     /locus_tag="VFV87_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5105242.1"
                     /translation="EETNLKCTILLDASKSMRYGGHPHPGPLSRGEGGKWSKFDYAAT
                     AAASLAYLLQQQQDAVGLVTFTSRVQKNLPPSSHPNHLKLLLHELEQTEPDEKTDVAA
                     VFPELARQIRRRGMIVLLSDLFLPVPTLAESLKQFRLRRHEVIVFHVMDNDELTFPFE
                     DNTLFRGLEEPVQLHTEPRALRRSYLEAVERFLGDVRKTCATAGVDYVLMNTKDPLDA
                     VLGSYLTFRQKIRRTARHH"
BASE COUNT          422 a          777 c          797 g          457 t
ORIGIN      
        1 gcgccccttg cccagcggct tttcggcgac cgccagcgaa tcgtcggcgt cgctgaaccg
       61 ggccaaaacg gaaacctctt tgccgagctg ctccggcttg accgccgaac cccaccagcg
      121 gaagaccttc accgaatcca gcagcggatt gttctggcca gcgaattcca caaacacttc
      181 gtgattcgcg ttctccacct tcaggttgac ccaggtccgc tcggtttcgt cgcccaggat
      241 gttcgtcagc cgcacgggcg aaagccccgc gccgtccttg taatactgct cgctatagaa
      301 gtgctcgtcg atctggtcgc cgggcatgat caccaggccc ccgccgttct ccacccactt
      361 ccggaggcgc tcgatgttct cggctgtctt gtcgcccagg cggtagttgt tgcagagaaa
      421 gatgacctgg tacttgccca aatcggcggc ctccagctcg ttttccgtga tgatctgcac
      481 ggcgacgccc gaggcggtcg gtcccggcgg ggcaagattc cgccgcaagt agaacgactc
      541 ggccttgccg aagtcggcgc tcggatcgcc gtcgatgatc agcactggaa ttccccgcac
      601 cagccgggcc gggaagaaag ccacgctatc agcttgcagg cgatcgtcct cgccctgccg
      661 accggtcagc aattccacgc gcacctgcct cggcgggagc tggcggcggg cgtcctccgt
      721 cggctcttcg ccgccggaga aggtgaaatt gaagcggacc gaggtttctt cgccggggcc
      781 gagccggtcg atcgtcgttt ccaccggcag ggcgtcgccg gcgctgaatt tcacgcgcaa
      841 atcgcgggcg tcggtggcgc cgtggttctt gacgcccacg tcgaaccgcg aagcgacgcc
      901 ctggacgagc gtcccttctg ggcggacctc ggtgatcgtg aggttgcggt cctcgtcgtc
      961 gcccgcgtcg attacaaagc agccctgcac gagcttcgac agccagccaa gcaccttgac
     1021 cggtgcttca ggggccgtgt cgctttcttt ccagtcgcgg cggcgcagat cggtgaggag
     1081 atagacgacg cggttcacgt tgccgggctg cgacgagagg tacgattcga cctccttgag
     1141 cgcggcatcg aggttcgccg ggcggtcgcc cgcttccaga cgctcgatcg tgacgttgat
     1201 ctcgtcgatg ctgtcctggc tgagatgggc ggcgctgaag agcggctgat tggggcggga
     1261 cgtgagaaag agcgtgagcg agttgtcggc cgactggtcc tgtgccaggc cgcccgtcag
     1321 gtcgatcagc cgtttctgag ccgagtccca ggccgacaag ttgccgagcc gggcctgcat
     1381 gctgagcgag tcgtccagga gcacgatcct ctcgaactgg gcggcgttga tcaggccggc
     1441 ggtgacgctc gtcggcagaa agggccgggc caggagcaga gcaatcagca gcaccgccag
     1501 gcagcgcaag agcagcagga gcaagttctc cagccgaatc cgccgccggt tcttcttgtc
     1561 ggcgtccagc aggaactcca tcgccgccca atcgacgatc ttgaactttc gcttgttgag
     1621 caggtggatg atgatcggca gcgagacggc cgccgctccc gcgacgaaca gccacggatt
     1681 aaccatccag gtggcgagcg attcgagctg ggcgacgacg aagggcatga ggacggctta
     1741 gtggtgcctc gccgtgcggc gaatcttctg gcggaaggtg aggtagctgc ccagcacggc
     1801 gtcgagcggg tctttcgtgt tcatgaggac gtaatcgacc ccggcggtgg cgcaggtctt
     1861 gcggacatcg cccaggaacc gctcgaccgc ttccaggtat gagcgccgca gggcccgcgg
     1921 ctcggtatgg agctggaccg gttcttccag gccgcggaac agcgtgttgt cttcgaaggg
     1981 gaaggtcagc tcgtcgttgt ccatgacgtg gaacacgatc acttcgtgcc gccgcaagcg
     2041 aaactgcttg agcgattcgg ccagcgtagg aaccggcaaa aacagatccg acagcagcac
     2101 gatcatcccg cggcggcgaa tctgccgggc cagctcagga aagaccgcag cgacatctgt
     2161 tttctcgtcg ggctccgtct gctccagctc atgcaacagc agcttgaggt gattcggatg
     2221 cgagctggga ggcaggtttt tctgaacgcg actggtgaag gtgaccaacc ccacagcatc
     2281 ttgctgctgc tggagcagat acgccaggct cgcggccgcc gtcgccgcgt agtcgaactt
     2341 gctccacttc cctccctctc cccttgagag agggccgggg tgagggtgcc cgccgtatcg
     2401 catcgacttg ctcgcgtcga gcaggatcgt gcatttgagg ttggtctcct cct
//
LOCUS       DASUGB010000822         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_645490, whole genome shotgun sequence.
ACCESSION   DASUGB010000822 DASUGB010000000
VERSION     DASUGB010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645490"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFV87_15595"
     CDS             <1..324
                     /locus_tag="VFV87_15595"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5105243.1"
                     /translation="MMVATYASRFDEFSGERFEILQTLEQPPASKIIKTRVRQSNGKA
                     VSLSYLVRLVGAEWKVVDVFLNGTISELASRRAEFTAILKSGGPDALIISLRHQGDKL
                     LAGGC"
     gene            complement(576..866)
                     /locus_tag="VFV87_15600"
     CDS             complement(576..866)
                     /locus_tag="VFV87_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HEX5105244.1"
                     /translation="MSQRIEAIFQNGTFRPQVPVSLAEGQRVSLSVESRPAASHPAGA
                     SGLGDLQDLLDTEFMESCRAHAEPPPLEEVRQILSAVPGSLADWNSQERDER"
     gene            1104..2570
                     /locus_tag="VFV87_15605"
     CDS             1104..2570
                     /locus_tag="VFV87_15605"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX5105245.1"
                     /translation="MSIARSVCAALVAVGVVSLLAGQKVVAGETPFGTERMAATHLIA
                     ADTPYYKSGPQQGRPADGTLKAGTRVQLVKAAGSFSQVKADSGVVAYVAAQSLTPVLS
                     KEAITPDVKNIATSINGFAFDLYREVGKQEGNLFVSPASISTALAMTYAGANGQTEKE
                     MAHVLHFDRIPTQLPVERFHEAYGKLTTLLNSGGGQGGYVLSTANRLWGQESYEFRPA
                     FLKITREHYGAELAELDFRRTEAARTTINEWARQQTRNKIQDLIPSGVLTSDTRLVLT
                     NAIYFLGGWAKEFSPAATKNQPFFVKPGESVEVPLMTQKRSFQYAETDDAQLLALPYR
                     GFEISMLVLLPKERGGLERLESQLTADKVEAWLAPARPREVEVYLPKFKLTTEVSLKE
                     TLSALGMPTAFGEAADFSKMASGEQLFISEVLHKAFVDVNEKGTEAAAATAVGVAAAA
                     FVEPPQPVVFRADHPFVFLLRDNRSGNILFLGRVSDPR"
     gene            2621..>3161
                     /locus_tag="VFV87_15610"
     CDS             2621..>3161
                     /locus_tag="VFV87_15610"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105246.1"
                     /translation="MKTPQISRANSGSLSRRALLRAGSLGLGGLGLSGLLRMQDAQGA
                     DQAAAFGKAKRVILLYLYGAAAQHETWDPKPEAPLEIRGKFQPAATSVPGIQIGEHLP
                     RVAKIADRLCFVRSMSHPYNIHSAAYTLTGIDKVDIPMELNPYDSRHWPSFGSVLDYL
                     AQRGEWNDEATRRNATEGVP"
BASE COUNT          618 a          979 c         1018 g          546 t
ORIGIN      
        1 atgatggtcg caacctacgc cagccgattc gacgaattct ccggcgagcg gtttgaaatc
       61 ctgcaaacgc tggagcagcc gccggccagc aagatcatca agactcgcgt gaggcaatcc
      121 aacgggaagg ccgtttcgct cagctacctc gtgcgacttg tgggcgctga atggaaagtg
      181 gtcgacgtgt tcctcaacgg caccatcagc gagctcgcga gccgccgggc cgagttcacg
      241 gcgatcctga agtcgggtgg gcctgacgct ctgatcatct ccctgcgcca tcagggagac
      301 aagcttctgg ccggcggctg ctgaccgcgg cgacgagcag gaggccagag gccacgtcgg
      361 cttccaaccg ggcgatcaga cggtggaaat ccggctccgt cagagtccct tcccgcacga
      421 gacgggagaa acaagaatga agctcgacca agtccaggcg cgaaataaag aattgattgc
      481 ccggcgcatc gaacaatgct tccacctggg gcgtgccgct ttcccgatga tagcgcttca
      541 ccagcgcact ggagtcgaga aaataacgcg gcatcctagc gctcgtcccg ctcttgggaa
      601 ttccaatccg ccagcgagcc ggggacagca ctcagtattt gccgcacttc ttcgagcggc
      661 ggcggctccg cgtgcgcccg gcacgattcc atgaactccg tgtcgagcaa atcctgcagg
      721 tcgcccagac cgctcgcccc cgcggggtgc gatgcggcgg gtcgcgattc gacgctgagc
      781 gacacccgct gcccctccgc aagggacacg ggaacctggg ggcggaacgt tccgttttga
      841 aaaatggcct cgatgcgctg agacatatca gcattctacc gggctgaagt tgatgctgct
      901 atagagcgtc ttattcctag cgaggcggtg gccctgaagc gacgaagcaa acgactcgcg
      961 ctagggcgca aagtgggccg attgctttag caggcaaaac atcgctccgg ggaaatcgag
     1021 ccggcgctcg cacgaaagct cgccacgaaa gctggtacaa ttaccgcctc taatcttttc
     1081 ccctcagttc gcggaggctg gccatgtcga tcgcgcgatc tgtctgtgcg gcgctagtgg
     1141 cggttggagt cgtctcgctt ctggcggggc aaaaggtggt ggctggcgaa acgccttttg
     1201 gaaccgagcg gatggcggca acgcatctga tcgcggcgga cacaccgtac tacaagtcag
     1261 gcccgcagca aggccggccg gcggacggca cgctcaaggc gggaacgcgg gttcagctcg
     1321 tcaaagcagc cggcagcttc tcgcaggtga aagccgattc cggcgtggtc gcgtatgtcg
     1381 ccgcgcagtc gctcacgccc gtgctcagca aggaagcgat cacgcccgac gtgaagaaca
     1441 ttgccacgtc gatcaacggc tttgcctttg atctctaccg cgaagtcgga aagcaggaag
     1501 ggaacttgtt cgtctcgccc gccagcatct cgaccgccct ggcgatgacg tatgcgggcg
     1561 cgaacggcca aaccgagaag gagatggccc acgtgctgca tttcgaccgg atcccaacac
     1621 agctaccggt cgagcggttc cacgaggcgt acggcaagct gacgacgttg ctcaatagcg
     1681 gcggcggcca ggggggctat gtgctctcca ccgcgaatcg cctctggggg caggagtcgt
     1741 atgagttccg tcccgcattc ctgaagatca ctcgcgaaca ttacggagcg gagctggccg
     1801 aactggattt ccgccggacc gaggccgccc gcaccaccat caacgagtgg gcccgtcaac
     1861 aaacgcggaa caaaatccag gatctcattc cctccggcgt gctgacttcc gacacccgcc
     1921 tggtgctgac caatgccatc tactttctcg gcggctgggc caaggagttt tcgccggcag
     1981 ccaccaagaa tcagccgttc ttcgtgaagc cgggcgagag cgtcgaagtg ccgctgatga
     2041 cgcagaaacg gagttttcaa tacgccgaaa ccgacgacgc gcagctattg gccttgccgt
     2101 accgcgggtt cgagatttcc atgctcgttt tgctgccgaa ggaacgcggc ggcctggagc
     2161 ggctcgaaag ccaattgacg gccgacaagg tcgaggcgtg gctcgccccc gcccggcccc
     2221 gcgaagtgga ggtgtacctg ccgaagttca agctgacgac ggaggtctcg ctgaaggaaa
     2281 cgctctccgc cctggggatg cccacggcat ttggcgaggc cgccgatttc tcgaaaatgg
     2341 ccagcggcga gcagctcttc atttccgagg tgctgcacaa ggcctttgtc gatgtcaacg
     2401 agaaggggac cgaagcggct gccgccacgg cggtcggcgt cgcggcggcg gcgtttgtcg
     2461 agcccccgca gcccgtggtg ttccgcgcgg atcatccgtt cgtgttcctg ctgcgggaca
     2521 accgcagcgg gaacatcctg tttctggggc gcgtcagcga tccgagatag ctccggaaaa
     2581 cacttttccc gcctttggcc acacggctac aatgggcggc gtgaaaactc cgcagatctc
     2641 acgtgcgaac tccggcagcc tgtcgcggcg ggcgctgctc cgcgcgggga gcctggggct
     2701 gggcgggctg gggctgtccg gcctgttgcg gatgcaagac gcccaggggg cggatcaggc
     2761 ggcggcgttc ggtaaggcga agcgagtcat tttgctgtac ctgtacggtg cggcggccca
     2821 gcatgagacg tgggacccga agcccgaggc gccgctggaa atccgcggca aatttcagcc
     2881 ggccgcgacg agcgttcccg gcatccagat tggcgagcat ctgccgcggg tggcaaaaat
     2941 cgccgaccgg ctctgcttcg tgcggtcgat gtcgcacccc tacaacatcc attcggcggc
     3001 atacacgctg accgggatcg acaaagtcga tatcccgatg gagctgaacc cgtacgattc
     3061 gcggcattgg ccgagtttcg ggtcagtgct cgattacctg gcgcagcgcg gcgagtggaa
     3121 tgacgaggca acgcggcgga acgccacaga gggcgttccc t
//
LOCUS       DASUGB010000823         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1620325, whole genome shotgun sequence.
ACCESSION   DASUGB010000823 DASUGB010000000
VERSION     DASUGB010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620325"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFV87_15615"
     CDS             <1..762
                     /locus_tag="VFV87_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5105247.1"
                     /translation="AGRIGPRTRAVSVSHITSPTALVLPVGEICAAAREHGVLAVVDG
                     AHAPGQVPVDLDALGADVYAGNCHKWLCAPKGAAFLHVRRDRQENIRPLVISHGANSS
                     RTDRSRFLIEFGWMGTGDPSAALSVPEAIRVMGSLLPGGWPGVMRRNRELAVAGRRIL
                     CESLGIQPPCPDEWLGSLASLPLPDAATPTPSKSPLYLDPLQDWLMENGKIEIPVIPW
                     PAPPKRLIRISAQLYNSLPQYRHLAQALKKNLQPT"
     gene            838..2370
                     /gene="xylB"
                     /locus_tag="VFV87_15620"
     CDS             838..2370
                     /gene="xylB"
                     /locus_tag="VFV87_15620"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX5105248.1"
                     /translation="MKTLIIGVDSGTQSTKVLIVDAKNGKVLGSAARAYDLLPGLPPG
                     AKEQHPHTWRDATAAAIRQALRDARANPADVRAIGVSGQQHGFVPLDSHGDVIRPAKL
                     WCDTSTAAECDEITEALGGTRKTIKTLGNAVLPGFTASKILWLKKHEPKNYARLASVL
                     LPHDYLNFWLTGRKVMEYGDASGTALLDVRKRRWCQPALDAISPDLANKLPPLITSDT
                     SVGPLQPATAKQLDLKTDVLVSAGGGDNMMGAIGTGNTREGVITASFGTSGTIYACSE
                     SPVVDPEGEIAAFCDSTNQWLPLLCTMNVTVATEMVRQDFEWTHETFATKAASVPPGS
                     RGLLLLPYLEGERTPNIPDGTGVMIGINQRTFSASHYARAAIEGVTLGMNYGLRRLAE
                     LGVKPAQIRATGGGAKSKVWRQIMADVFNTEVVTLKVSEGAAYGAALQALWCWRREKG
                     EKVGISDITDEFVEVNSMETAVPDAKAAAVYQELQKLQDATSLALREVFKQHRKFVLA
                     AR"
BASE COUNT          502 a          728 c          833 g          494 t
ORIGIN      
        1 gcgggcagga tcggtccacg gacgcgcgcc gtgtctgtgt cccacatcac gtcgccgaca
       61 gcgctcgttc tgccggtggg cgagatctgc gccgctgcgc gggagcacgg agtgctcgcg
      121 gtcgtggacg gcgcccatgc gcccggccag gtgccggtcg acctcgacgc gctcggcgcg
      181 gacgtgtacg cggggaactg ccacaaatgg ctgtgtgctc cgaagggcgc ggcgtttctg
      241 cacgtgcgtc gggatcggca ggaaaacatc cggccgttgg tgatcagcca cggggcgaat
      301 tcatcgagaa cggaccggtc tcgttttctc atcgagttcg gatggatggg aaccggtgat
      361 ccatcggcgg cgttaagtgt gcccgaggcg attcgagtga tggggtcgct tcttcccgga
      421 ggatggcccg gagtcatgcg gcgtaatcgg gaactggctg tcgccggccg gcggatcctt
      481 tgcgagagcc tgggaataca gccgccctgt cccgatgaat ggctgggttc cctcgcatcg
      541 ctgcccttgc ctgacgcggc gacccccacg ccgtccaaat caccgctcta cctcgacccg
      601 ctgcaggatt ggctgatgga gaatggaaaa attgaaatcc cggtcatccc ctggccggcg
      661 ccgcccaagc gcctcatccg catctccgcc cagctttaca actcgctgcc acaataccgg
      721 cacctggcgc aggcgttaaa aaagaatctg cagccgactt aggcatgctt cgtcgggagc
      781 ctgagacaag gatggacagg tgcagatcgg ccagtattct tcggtcactt ttccaacatg
      841 aagacactga taatcggcgt tgatagcggg acgcagtcga ccaaggtcct gatcgtggac
      901 gccaaaaacg gcaaggtcct cggcagcgcc gcgcgcgctt acgatttgct tcccggcttg
      961 ccgcctggcg ccaaagagca gcatccgcac acctggcgcg acgccacggc cgcggcgatt
     1021 cgccaggcgc ttcgtgatgc ccgcgcgaat cctgccgacg tccgggccat cggggttagc
     1081 ggccagcagc atggttttgt tccgctcgat tctcatggcg atgtcattcg gccggccaaa
     1141 ctttggtgtg atacatccac cgcggccgag tgtgatgaga tcaccgaggc attgggcgga
     1201 acccgcaaaa ccatcaagac gctcggcaat gctgtattgc ccgggttcac cgcgtcgaaa
     1261 atcctttggc tgaaaaagca tgagcccaag aactatgccc ggcttgcctc ggtattgttg
     1321 cctcatgatt atctgaattt ctggctgacg ggtcgcaagg tgatggaata cggcgacgcc
     1381 agcggcacag cgcttctgga tgttcgcaaa cgtcgctggt gccagcccgc gttggatgcg
     1441 atcagtcccg atctagccaa caagctgcct ccgttgatca ccagcgacac ttcggtgggg
     1501 ccattgcagc ccgcgacggc caaacaactg gatctgaaga cggacgtcct ggtcagcgcc
     1561 ggaggtggcg acaacatgat gggagcgatt ggcactggca acacgcgaga gggggtcatt
     1621 actgcgagct tcggcacgag tggcacgatc tacgcctgct cggaaagtcc ggtggtggat
     1681 cccgagggtg agatcgcggc attttgtgat tccacgaacc aatggctgcc gctgctttgc
     1741 accatgaacg tcacggtggc gacggaaatg gtgcgccagg attttgagtg gacgcatgaa
     1801 acatttgcca ccaaggcagc atcggtgccg cccggctcgc ggggacttct tctgttgccc
     1861 tacctggaag gggagcgaac gccgaacatc cccgacggca cgggcgtgat gattgggatc
     1921 aatcaacgga cattcagtgc gagccactat gcgcgggcag cgattgaagg cgtgacgttg
     1981 ggaatgaatt acggcttgcg tcgactggca gagttgggag tcaagccggc gcagattcgg
     2041 gcgacgggcg gcggggccaa gtcgaaggtg tggcggcaaa tcatggccga cgtgtttaac
     2101 accgaggtcg tcaccctgaa agtgagcgaa ggcgcggcgt atggtgctgc tctgcaagcc
     2161 ttgtggtgtt ggcggaggga gaagggtgaa aaggttggca ttagtgatat cacggatgaa
     2221 ttcgtggagg tgaattccat ggagacggcg gtgcccgacg cgaaagcggc ggctgtgtat
     2281 caggaattgc agaagttgca ggacgcgacg tccctggcct tgcgggaagt cttcaagcag
     2341 catcggaaat ttgttttggc cgcaaggtag gcgactgtct caaaccaagg gggcggaccg
     2401 cggctcttcg agccgcagca acgtccgggt tcgcaacgac tcacccccgc cttaaattca
     2461 ttgcgcccag agtcgttgaa tgatccaacg ccgccttcgc gttgctggtt tttacagtag
     2521 tgccgccggc taagcgaatt tttggtgtac ggaaagg
//
LOCUS       DASUGB010000824         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_230826, whole genome shotgun sequence.
ACCESSION   DASUGB010000824 DASUGB010000000
VERSION     DASUGB010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230826"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFV87_15625"
     CDS             <1..545
                     /locus_tag="VFV87_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5105249.1"
                     /translation="ILVGEMRDLETIEAAITAAETGHIVFATLHTNSAQGTVNRMIDA
                     FPGNLQDQIRTQLSTALIGVVAQTLLPKIGGGRIAAYEILVVTPGIANLIRENKTFRI
                     NSAIQTGSKFGMQLMDDALFKLWREEKVIIEEVLAKAHNPDELAKRIVNYQRGIMEDP
                     AAAGVKPGEDLNAPPKPGGH"
     gene            complement(673..1953)
                     /locus_tag="VFV87_15630"
     CDS             complement(673..1953)
                     /locus_tag="VFV87_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5105250.1"
                     /translation="MAIRFVGLLNVAALCLLGVVAGLEGAETPATPAAVGQWPQHLGP
                     DRNGVSRETGLIDKWPAGGPREVWRMKGGAGMSGLAISRGRVLTLVQKQGQQFLVALN
                     ADTGQPIWETAIASEYRNQMGNGPRATPTIAGERAFAYTGEGILAAVNFGDGTLIWSH
                     DLPKELGGEPADYGMACSPLVVGERVIVQVGAPQAAVAALDGASGKLVWKAGDDPAGY
                     SSPALLRVSGQEQVVSFTGGSVMGLAPDKGAVLWRHPYETDFACNIATPLSIKGNVFV
                     SAGENHGSVLVGVQPDGGFAVKELWSSQGGGSVLRCEWQTPVLLDGYLYGFDNVGSAG
                     PVTHLTCIEAGTGKRMWQQLRFGKGNLIAAEGKLLISTMKGELVVARATPEKYEEIGR
                     GVVIGTTRQAPALAGGLLYLRDDKEIVCLDIRQK"
     gene            2176..>2405
                     /locus_tag="VFV87_15635"
     CDS             2176..>2405
                     /locus_tag="VFV87_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105251.1"
                     /translation="MRRWPLAALLAAALCAGCGRHEAAPAAKAGKGELPVIQAAVFRV
                     EPTAWPTIIKSQGSLVADEVTVVGAKVAGRVS"
BASE COUNT          435 a          854 c          693 g          423 t
ORIGIN      
        1 tgatcctggt cggcgaaatg cgcgacttgg aaacgatcga agcggcgatc accgctgccg
       61 aaaccgggca catcgtgttt gccaccctgc acaccaacag cgcccaggga acggtcaacc
      121 gcatgatcga cgcctttccc ggcaacttgc aggaccagat ccgcacgcag ctttccaccg
      181 ccctgatcgg cgtcgtcgcg cagacgctgc tccccaagat cgggggcggg cgcattgccg
      241 cttacgaaat cctggtcgtc actccgggca tcgccaactt gatccgcgaa aacaaaacct
      301 tccgcatcaa ttccgccatc cagaccggct cgaaattcgg catgcaactc atggacgacg
      361 ccctgttcaa gctgtggcgc gaagaaaagg tcatcatcga ggaagtcctg gccaaggctc
      421 acaaccccga cgagcttgcc aagcgaatcg tcaactacca gcgcggcatc atggaagacc
      481 ctgccgccgc aggcgtcaaa ccgggcgaag acctgaacgc ccccccgaaa ccaggcggcc
      541 actaggaatt ctggatttgc gatttcggat tttggattgc agcggctatt gatcacggac
      601 tcctgaccac tgaaacctga aaccttgaac ctgaaacctt gaacttaaac cctgcggccc
      661 ggccgattct cgtcactttt ggcggatgtc caggcagacg atttccttgt cgtcgcgcag
      721 gtaaagcaga ccgccggcca gcgccggggc ttggcgcgtt gtgccgatga ccacgccgcg
      781 gccgatttcc tcgtatttct cgggcgtcgc tcgagcaacg acgagctccc ccttcatcgt
      841 cgagatcagc agctttccct cggccgcgat caggttccct ttgccgaagc gcaattgctg
      901 ccacatccgc ttgcccgtgc ccgcttcgat gcacgtcaag tgcgtcaccg gcccggcgct
      961 ccccacgttg tcgaagccat acaggtagcc gtccagcaac accggcgtct gccattcaca
     1021 ccgcagcacg ctgccgcccc cttgcgaact ccacagctcc ttcaccgcaa aaccaccgtc
     1081 cggctgcacg cccaccagca ccgagccgtg gttttcgccg gccgacacga agacgttccc
     1141 cttgatcgac agcggcgtgg caatgttgca ggcgaaatcg gtctcgtagg gatgccgcca
     1201 gagaaccgct cccttgtccg gcgccagacc catcaccgag ccgccggtga acgatacgac
     1261 ctgctcctgc ccgcttaccc gcagcagcgc cggcgacgag tatcctgccg gatcgtcccc
     1321 cgctttccag acgagtttgc ccgaggcgcc gtccagcgcg gcaacggccg cctgcggcgc
     1381 tccgacttgc acgatgaccc gctcgcccac gaccagcggc gaacacgcca tgccgtagtc
     1441 ggccggctct ccgccaagct ccttcggcaa atcatgggac caaatgagcg ttccgtcgcc
     1501 gaaattgacc gccgccagaa ttccttcgcc cgtgtaggcg aatgcccgct cgccggcaat
     1561 ggtgggcgtc gcccgcggcc cgttgcccat ctggttgcga tattccgaag cgatcgccgt
     1621 ttcccagatc ggctgacccg tgtcggcgtt cagggccacg aggaattgct gcccttgttt
     1681 ttggacgagc gtcagcacgc ggccgcggct gatcgccagc cccgacatgc cggcgccccc
     1741 cttcatccgc cagacttccc gcgggccccc cgccggccac ttgtcgatga gtcccgtttc
     1801 gcgcgagacg ccgttgcggt ccggccccag gtgctgcggc cactggccca cggcggccgg
     1861 cgttgcaggc gtctccgccc cctcgaggcc ggcaacaacc cccagcaggc acagcgccgc
     1921 gacattcagc aggccgacaa atcggatcgc catcccggaa actcctgcgc aatgcgtata
     1981 gccgcaattc cagtccatca ccgtagcgta tccccgcgct gagcgacagg cgatccattc
     2041 gctttcgttg ttatgtagtg gtataaaaag gagggccaac agagccgcgg cggcagcatt
     2101 tcgcggcatg gacctgcctg gggtgggagt tgttcgatgc ttgatcgtcg gtgtcccgtg
     2161 gtctgtctcc attcgatgcg acgttggcct ctggcggcgc tgctggcggc tgctctttgc
     2221 gccggatgcg gccgccacga ggctgcccct gccgcaaagg cgggaaaggg agagctgccg
     2281 gtcattcaag cggccgtgtt tcgcgtcgag ccgaccgcct ggcccaccat catcaagagc
     2341 cagggcagcc tcgtggccga cgaagtgacg gtggtcgggg ccaaggtcgc cggccgcgtc
     2401 tccga
//
LOCUS       DASUGB010000825         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1063636, whole genome shotgun sequence.
ACCESSION   DASUGB010000825 DASUGB010000000
VERSION     DASUGB010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063636"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1649
                     /locus_tag="VFV87_15640"
     CDS             267..1649
                     /locus_tag="VFV87_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5105252.1"
                     /translation="MTQLPAAKIKEFKASFQGSVVCQGDADYDETRQIWNAMIDRRPA
                     AIARCTATAHVVQAVQFARQNKVLVSIRGGGHNIAGNAVCDDGLMIDLSPMKQVQVDT
                     AARRATVEPGCILSDFDAAAQAHGLATPLGINSTTGVAGLTLGGGFGWLSRKFGMTID
                     NLLSAEVVTAEGRVLRASETENADLFWGLRGGGGNFGIVTRFEFQLHPVGPNVLSGLI
                     VFPFQQAKSVISQFARFTLTMPDELNVWLVTRQAPPLPFLPADVHGKEVVVLALCYAG
                     DPEAGKKLIEPLRTFGTIVGEHVGVQPYVAWQQAFDPLLTPGARNYWKSHNFTQLSEG
                     AIDAIIEYAGQLPSPQCEIFVGTIGGQTARVAPEKMAYSSRDANYVMNVHGRWDSAAD
                     DARCIAWAREFFAKSQPFASGGAYINFLTQDETDRVAFAYSTTYNRLVELKRRYDPTN
                     FFRMNQNIQP"
     gene            complement(1693..3090)
                     /locus_tag="VFV87_15645"
     CDS             complement(1693..3090)
                     /locus_tag="VFV87_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5105253.1"
                     /translation="MKDTPKTADPSSTRLKILFADDEPALQELMHLELPRMGHEVTIC
                     PDGHTAVAALEKNTYDCIIVDLDMPGFNGIQVIGRCKESSPDTDAVVLTGKSSFDTAV
                     QALRHGAVDYLTKPCKLLDLKALLLRVARQREWMNKCRALAHQLERIEGKTELVGTSR
                     GMEQVKRLIEKVAPTNSTVLILGETGTGKELVARAVHQNSLRADRPFVAVNCGALPES
                     LIESELFGHRKGAFTGADDHRVGLFEVAHGGTIFLDEIGELPKSMQAKLLRVLESGEI
                     RRVGDNDAFEVDVRVVCATHRNLPEMVEAGNFREDLMYRINTFEIRLPSLRERTGDIE
                     ALANHLFRRFRPSLKADATLFTPDAIDLLEKHTWPGNVRELANVIEHATILCEAPPIT
                     PDHLPQRFASRRGPLPKVRITGPITLRDLEMQAIYDALDRHDGNKPEAAEELGISLKT
                     LYNKLNQTALMEKAG"
     gene            complement(3140..>4439)
                     /locus_tag="VFV87_15650"
     CDS             complement(3140..>4439)
                     /locus_tag="VFV87_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5105254.1"
                     /translation="ESKLEADIRFGDRSHERKTIAALEESLAELKALQDNHTWVKEES
                     DVIRLADRVDEMYDLAGTLPKELHDRMHDFAQQVKTEYRTWIFLEWTATIGAAIFLPV
                     ILFLFYDFLFRPLNVLITGSRRIAQQDDFDHRIHLRSRDEMAELATALNDMTARFQEI
                     RSDLDEQVQQRTKEVVRSEQLASVGFLAAGVAHEINNPLASIAWSAESLESRLYDILG
                     AGADESAGQEQSEVEILRKYLRRIQDEAFRCKGITEKLLDFSRLGDVEKQDTDLGDLV
                     QGVIDMVRHLGKYREKKIELISPEYVVAPINAQEMKQVVLNLITNSLDSLDHGGTVWI
                     SLRKNGPWAELVVRDDGCGMSPEVLRHLFEPFFTRKRDGSGTGLGLSISWRIVTDHGG
                     TIEPSSDGPGKGARMRVLLPLVAKDKFVPHRKPAIQLAAA"
BASE COUNT          725 a         1333 c         1498 g          883 t
ORIGIN      
        1 atctcgccgg ctaaccttgc gcgccttcgt ccgttcccta gagatgcgcg aactgtacga
       61 attagtcatc gcttgtaatc agtttttctc aaaatgatgc cgctgtgcta atggccgttt
      121 cgaacctgtc ggcccgccca ctgtgtttgt ccgggacgcg cggacgccaa tgagcggcgt
      181 tcctgaggcc ggtttcgaga gagacgatct gggggcataa tgcgccgagg agaccgttct
      241 gcgagaacca tccaacggag ggaattatga cgcagttgcc cgcagccaag atcaaagaat
      301 tcaaagcgag ttttcagggc agcgtggttt gccaaggcga cgccgactac gacgagaccc
      361 gccaaatctg gaatgcgatg atcgaccggc ggccggcggc catcgctcgc tgcacggcga
      421 ccgcccatgt ggtccaggcc gtccagtttg cccgccagaa caaggtgctg gtctcgattc
      481 gcggaggagg tcacaacatc gcgggcaacg ccgtctgcga cgacggtctg atgatcgacc
      541 tttcccccat gaagcaggtg caagtcgata ccgcggcacg ccgcgcgacc gtcgagcccg
      601 gctgcatcct gtccgatttc gacgccgccg cccaggcgca tggcctggcc acgccgctgg
      661 gcatcaattc gaccaccggc gtcgccggct tgacgctcgg aggcgggttc ggctggctaa
      721 gccggaaatt cggcatgacg atcgacaatc tgctgtcggc ggaggttgtc acggcggagg
      781 gccgcgtgct gcgcgccagc gaaaccgaaa acgccgattt gttctggggg ctgcggggcg
      841 gcggcgggaa ttttgggatc gtcacccgct tcgagttcca gctccatccc gtcgggccca
      901 atgtgctgag tgggttgatc gtctttccgt tccagcaggc gaagtccgtc atttcgcagt
      961 tcgctcgctt caccctcacg atgccggacg aactcaatgt gtggctcgtc actcggcagg
     1021 cgccgccgct tcccttcctg ccggcggacg tccacgggaa agaggtcgtg gtgctggcct
     1081 tgtgctacgc gggcgacccg gaggcgggaa agaagttgat cgaaccgctc cgcacgttcg
     1141 gaacgatcgt cggcgagcac gtcggcgtgc agccgtatgt tgcctggcag caggccttcg
     1201 atccgctgct gacgccgggc gcccgcaact attggaaatc gcacaatttc acgcagctca
     1261 gcgaaggggc gatcgacgcc atcatcgagt acgcgggcca gctcccttcg ccgcagtgcg
     1321 agatcttcgt cggcaccatc ggcggccaga cggcccgcgt cgcccccgag aaaatggcct
     1381 attccagccg cgatgccaac tatgtgatga acgtccacgg acggtgggac tcggctgcag
     1441 atgacgcccg ctgcattgcc tgggcgcggg aattcttcgc caaatcgcag ccctttgcca
     1501 gcggcggcgc ctacatcaac ttcctcaccc aggacgagac cgatcgcgtc gcgtttgcct
     1561 acagcacgac gtacaatcgg ctggtggaat tgaagcggag gtacgatccc accaacttct
     1621 tccggatgaa tcagaacatc cagccgtgac cgcctcccgg cggcggatct cttgaggcga
     1681 tcatgactcg tgttagcccg ctttttccat cagggcggtc tggttgagct tgttgtagag
     1741 cgtcttgagg ctgatgccga gctcttcggc ggcttcgggt ttgttgccgt cgtggcggtc
     1801 gagggcgtcg tagatcgcct gcatttccag gtcgcggagg gtgatcggcc cggtgatgcg
     1861 gaccttcggc aaggggccgc ggcggctggc gaagcgctgc ggcaggtggt cgggcgtgat
     1921 cggcggcgct tcgcagagga tcgtcgcgtg ctcgatcacg ttagccagct cgcggacgtt
     1981 gcccggccag gtgtgctttt ccagcaggtc gatcgcgtcg ggcgtgaaga gggtcgcatc
     2041 ggccttcagg ctcggccgga agcggcggaa gagatgattg gccagggcct cgatgtcgcc
     2101 tgtccgctcg cggaggctcg gcagccggat ttcgaacgtg ttgatccggt acatcaggtc
     2161 ttcgcggaag ttgccggctt cgaccatctc gggcagattg cgatgcgtgg cacagacgac
     2221 ccgcacatcg acttcgaagg cgtcgttgtc gcccacgcgg cggatttcgc cgctctccag
     2281 cacgcgaagc agtttcgctt gcatgctctt gggcaactcg ccgatctcgt cgaggaagat
     2341 cgtgccgccg tgggcgacct cgaacagacc gacgcggtgg tcgtcggccc ccgtgaacgc
     2401 gcctttgcga tggccgaaca gctcgctctc gatgagcgat tcgggaaggg cgccgcagtt
     2461 gaccgcgacg aacggccggt cggcccgcag gctgttctgg tggacggccc gggcgaccag
     2521 ttccttgccg gttcccgtct cgccgaggat cagcacggtc gaattggtcg gcgcgacttt
     2581 ctcgatcagc ctcttcacct gctccatgcc gcggctggtg ccgacgagct cggtcttgcc
     2641 ttcgattcgc tcgagctggt gggccagggc ccggcacttg ttcatccact cgcgctggcg
     2701 ggcgacgcgg aggagcagcg ctttgagatc gagcagcttg cagggcttgg tgaggtaatc
     2761 gaccgctccg tgccgcaggg cctgcacggc ggtgtcgaac gacgatttgc cggtgaggac
     2821 aaccgcgtcg gtatcgggcg acgattcctt gcacctgccg atgacctgga tgccgttaaa
     2881 ccccggcatg tcgagatcga cgatgatgca gtcgtaggtg ttcttttcga gcgcggcgac
     2941 agccgtgtgg ccgtcggggc agattgtcac ctcgtgcccc atgcggggca gttcgaggtg
     3001 catcagctcc tgcagcgccg gttcgtcgtc agcaaagagg attttgaggc gcgtggagga
     3061 aggatcggcg gttttcgggg tgtctttcat aacaacgttc gggatcgctg gagagcgttc
     3121 gtgacgagag gggaaaggtt caggcggcag cgagttggat tgcgggtttg cgatggggga
     3181 cgaatttatc cttggcgacc aggggcaaga ggacgcgcat ccgcgcgccc ttgccggggc
     3241 cgtcgctgga gggctcgatc gtgccgccgt ggtcggtgac gatccgccag ctaatcgaca
     3301 gccccaggcc cgtgccggag ccgtcgcgct tgcgggtgaa gaacggttcg aacaggtgcc
     3361 gcagcacttc gggcgacatg ccacagccgt cgtcgcgaac gaccagctcg gcccacggcc
     3421 cgttcttgcg gagcgaaatc cagacggtgc cgccgtggtc gaggctgtcg agcgagttgg
     3481 tgatcaggtt cagcacgacc tgcttcatct cttgagcatt gatcggggcg acaacatatt
     3541 ccgggctgat cagctcgatt ttcttttcgc gatacttgcc caggtggcgg accatgtcga
     3601 tcactccctg cacgagatcg cccaggtcgg tgtcttgctt ttcgacgtcc cccagccgcg
     3661 agaagtcgag cagcttttcg gtgattccct tgcagcggaa ggcttcgtcc tggattcgcc
     3721 gcaggtattt gcggaggatt tcgacctcgg attgctcctg gccggcggat tcgtcggcgc
     3781 cggcgccgag gatgtcgtac aggcgcgact cgagcgattc ggcgctccac gcgatcgacg
     3841 ccagcgggtt gttgatttca tgggccacgc cggcggcgag gaagccgacg ctggcgagct
     3901 gctcgctgcg gacgacttcc ttggtccgct gctggacctg ctcgtcgagg tcgctgcgga
     3961 tttcctggaa gcgggcggtc atgtcgttga gggccgtggc cagctcggcc atttcgtcgc
     4021 gcgagcggag gtggatgcga tggtcgaaat cgtcctgctg ggcgatccgc cgcgagccgg
     4081 tgatcagcac gttcagcggc cggaacagga agtcgtagaa caggaagagg atgaccggca
     4141 ggaagatcgc cgcgccgatc gtggcggtcc attcgaggaa gatccaggtg cgatactcgg
     4201 ttttgacctg ctgggcgaag tcgtgcatcc ggtcgtgcag ctctttgggc agggtgccgg
     4261 ccaggtcgta catttcatcg acgcggtcgg ccaggcggat gacgtcggac tcttccttga
     4321 cccaggtatg gttgtcctgg agcgctttca gctcggcgag cgattcctcc agggcggcga
     4381 ttgtcttgcg ctcgtgcgag cggtcgccga agcggatgtc ggcttcgagc ttcgattcg
//
LOCUS       DASUGB010000826         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1340820, whole genome shotgun sequence.
ACCESSION   DASUGB010000826 DASUGB010000000
VERSION     DASUGB010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340820"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1495)
                     /locus_tag="VFV87_15655"
     CDS             complement(29..1495)
                     /locus_tag="VFV87_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX5105255.1"
                     /translation="MPVAAVLGIDITTGEFALAVRSADGEEDYVAMKMQGDKYWDGDK
                     NYPAFELAAVAGMLLELLSDLERKGWTFNKAGRMSVSCRQHDMVLLGEAGQSVLPFAL
                     SWQCNAATAEADEINASRSAVKSIGTVEPRLILPKLLHVLRKQPELKAGLACVTTTGD
                     WVAYQLTGRQTINTSEAQSNGLFDLSKRKLPEKVLRSLGLKARWFPRLVKSGKSVGLV
                     TGSDVPAADGEWAALRAKLADWEFLAGMGDNHASAVGCGVKDSQSVLVSAGTSGTINW
                     VSKRFPPPKSGVLCFGFFLRNLWLLMLARCAKWYDDARDEFFPPDKFSHADLNRLALE
                     AEPTKLARVLFHDGEHYPRDWSRLPTSVQVASVQFSIVLELLLRLRQMLKAMPRKAAV
                     QRFILTGGLSQSDFFCHVFHTGVKLLVPDAEVLVSGRTGPLRYKTTAYGALVNAEFGG
                     DVAAIPDDRFPTNPCPAPDDSKRQSLEDRLRPLLTQDK"
     gene            complement(1599..2909)
                     /gene="xylA"
                     /locus_tag="VFV87_15660"
     CDS             complement(1599..2909)
                     /gene="xylA"
                     /locus_tag="VFV87_15660"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX5105256.1"
                     /translation="MPAAFPEITKIPYEGPQSKNPLAFRWYNAGEKVEGKTMAEQLRF
                     AVVYWHTFRGTGSDPFGAATMQRPWDDGSDSVENAQRRARAAFEFIEKLGAPFYAFHD
                     RDVAPEGKTLAESNKNLDAVVKVLKEEQQRTGIKLLWGTANLFSNPRYMHGAATTCNA
                     DAFAYAAAQVKKALEVTKELGGVNYVFWGGREGYQNLLNTNMKRELDHLARFFHLAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDSDAAACLNFLRAYDLLPHFKLNIETNHATLAG
                     HEMMHELEYAGHQGALGSIDANTGDLLLGWDTDQFPTNIYLTTQIMLVLLKYGLAPGG
                     VNFDAKVRRESFEPIDLFHAHIGGMDAFARGLKIAAAIRADGEFDKFVKERYSSFDGG
                     IGAEIESGKANFKTLETYMLQKGEIAPNQSGRQEMLEHLFNRYI"
BASE COUNT          536 a          969 c          999 g          607 t
ORIGIN      
        1 aagcagccat ggggctaact cctaccagct acttgtcctg ggtgagcagc ggacgcagcc
       61 ggtcttccaa actttgtcgc ttcgagtcgt cgggcgccgg gcagggattc gtgggaaagc
      121 ggtcgtccgg aatcgccgcc acatcgccgc cgaactcggc attgaccagc gccccgtatg
      181 cggtcgtctt atagcgtaac ggcccggtgc gtccgctgac cagtacttcc gcgtccggta
      241 cgaggagctt aacgcccgtg tgaaacacgt ggcagaagaa atccgactgg ctgaggccgc
      301 ccgtaaggat gaaccgttgc acggcggctt tcctcggcat ggctttcagc atttgccgca
      361 gacgcagcag cagctccagc acgatcgaga attgcacgct ggcgacctgg acgctggtcg
      421 gcaggcgcga ccagtcgcga ggatagtgct cgccgtcgtg gaacagcacg cgggccagct
      481 tggtgggctc ggcttcgagg gccaggcgat tgagatcggc gtggctgaac ttgtccggcg
      541 gaaagaactc gtcgcgggcg tcgtcatacc atttggcaca gcgggcgagc atcagcagcc
      601 acagattgcg gaggaagaag ccgaagcaca acacgcccga tttgggcggc gggaaccgct
      661 tgctcaccca gttgatcgtg ccgctggttc cggccgagac gaggacgctt tgcgaatcct
      721 tcacgccgca gccgacagcg gaagcgtggt tgtcgcccat gccggccagg aactcccagt
      781 cggcgagctt ggcccgcagc gcggcccact cgccgtccgc ggcgggaacg tcgctacccg
      841 tcacgaggcc gacggacttg ccgcttttga ccaggcgtgg aaaccagcgg gccttcagcc
      901 ccagcgagcg gaggactttc tcgggcaatt tgcgtttcga gagatcgaac aggccgttgc
      961 tctgagcttc ggacgtgttg atcgtctgcc ggccggtaag ctgataggcg acccagtcgc
     1021 cggtggtcgt cacacacgcc aacccggctt tcagctccgg ctgcttccgc aagacgtgca
     1081 gcagcttggg caggatcagc cgcggttcga ccgtgccaat ggacttcacc gccgagcgcg
     1141 aggcgttgat ttcgtcggct tcggcggtgg cggcgttgca ctgccagctc agggcgaagg
     1201 gcaagacgga ctggccggct tcgccgagca gcaccatgtc gtgctggcgg caggaaacgg
     1261 acattcgccc cgctttattg aacgtccagc ccttcctttc caggtcgcta agcagctcca
     1321 gcagcattcc cgccaccgcg gccagctcga aggctggata gttcttgtcg ccatcccaat
     1381 acttgtcgcc ctgcatcttc atggcgacat agtcctcttc gccgtcggcg ctgcggaccg
     1441 ccagggcgaa ctcgcccgtc gtaatatcga tccccaaaac cgcggctacc ggcatgtatg
     1501 gtcctccacg tcaaacctgc ccggcgacag tcgccgagat gccaaggtgt aaatcaagat
     1561 gcgagtgcgt gcaaacgggc agcagccggt tccacgcctc agatataccg gttgaacaag
     1621 tgctcgagca tctcctgccg gccgctctgg ttgggtgcga tctcgccctt ttgcagcatg
     1681 taggtttcga gcgtcttgaa attcgccttg ccggactcga tctcggcccc gatgccgccg
     1741 tcgaacgagc tgtaccgctc cttcacgaac ttgtcgaact cgccgtccgc ccgaatggcg
     1801 gcggcgattt tgaggccgcg ggcgaaggcg tccattccgc cgatgtgggc gtggaacagg
     1861 tcgatcggct caaagctctc gcggcggact ttggcgtcga aattcacgcc gccgggggcc
     1921 aggccgtact tgagcagcac gagcatgatc tgcgtcgtca ggtagatgtt cgtcgggaac
     1981 tggtcggtgt cccagccgag gaggagatcg cccgtgttgg cgtcgatcga accgagggcc
     2041 ccctgatggc cggcgtattc cagctcgtgc atcatctcgt ggccggccag cgtggcgtgg
     2101 tttgtctcga tgttcagctt gaagtgcggc aacaggtcgt aggcccgcag gaagttgagg
     2161 caggccgccg cgtcgctgtc gtactggtgc ttggtcggct ccttcggctt gggctcgatc
     2221 aggaattgcc ccgtgaagcc gatctctttg gcgtaatcga cggccaggtg gaagaagcgg
     2281 gcgagatggt cgagctcgcg cttcatattc gtgttcagca ggttctgata gccttcgcgg
     2341 ccgccccaga agacatagtt gacgccaccc agctccttgg tcacctccaa ggcttttttc
     2401 acctgtgccg cggcataggc gaaggcatcg gcgttgcagg tcgtcgccgc gccgtgcatg
     2461 tagcggggat tgctgaacag gttcgccgtc ccccatagca gcttgattcc cgtccgctgt
     2521 tgctcctcct tgaggacctt cacgacggcg tcgagattct tgttcgattc ggcgagtgtc
     2581 ttgccctccg gggcgacgtc gcggtcgtgg aaggcataga acggcgcacc gagcttctcg
     2641 ataaactcaa acgccgcgcg ggcccgccgc tgggcgttct cgacgctgtc gctgccgtcg
     2701 tcccaaggcc gctgcatcgt cgcggcgccg aaggggtcgc tccccgtccc gcggaacgtg
     2761 tgccaataga cgaccgcaaa gcgcaattgc tccgccatcg tcttcccctc caccttctcg
     2821 ccggcgttat accagcgaaa cgccagcgga ttcttcgact gcgggccttc gtaggggatt
     2881 ttggtgattt cagggaatgc ggcaggcatg ggagaggtgt gtgagtggtg agtggtgagt
     2941 ggtggccggg agctgcgcaa gtcgcggctg cctgcaatcg ttggtactga gtactcggta
     3001 ctctgcaacc actgccggtg gcaagcgagt catcgtgcca gattcagccg aaagccgcaa
     3061 cgggcggcga gcttagattc accacggcgg ctcaccccaa cctgcaatgt g
//
LOCUS       DASUGB010000827         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_786164, whole genome shotgun sequence.
ACCESSION   DASUGB010000827 DASUGB010000000
VERSION     DASUGB010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786164"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..302
                     /locus_tag="VFV87_15665"
     CDS             63..302
                     /locus_tag="VFV87_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105257.1"
                     /translation="MSPSYATALEPFVDRRNYENQAGPPQRERRQFTNSHEGLSPEAR
                     DLAQAIDSYKLMHRRRFITYEEMLAVVKSLGYRRD"
     gene            complement(337..1812)
                     /gene="gatB"
                     /locus_tag="VFV87_15670"
     CDS             complement(337..1812)
                     /gene="gatB"
                     /locus_tag="VFV87_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5105258.1"
                     /translation="MPDHPYQLIVGLETHVQLLTHTKLFCRCSTKFGAPPNTQTCPVC
                     IGLPGSLPVMNREAHRLGMVAAIALNCGIPAFTKWDRKQYYYPDLPKGYQISQYDLPM
                     SQHGWLEIHDPKSRFEPKKVGIIRAHLEEDAGKSLHDEAAGKADTRIDLNRTGTPLLE
                     IVSAPDIRSTEETRAYLNELKLLLTYLAVSDCNMQEGSLRVDANINIHIDTPQGKIAT
                     PIVEIKNLNSFRHIERALAYEASRQYQAWQETARTIKDAPKQTRGWDDAKGITFSQRE
                     KEESSDYRYFPDPDLCPVVTPPAEVEQVRGSLTELPRNIRQRLEQAYGITPYDSDVIV
                     AQGRPLVDYYEAVATASGDGKKAANWVTQEVLRTLNEQGWNLEQFPLTAAALAELVKL
                     VVAGEIEVTRAKEVFNDMVANKRSAAQSMQALGIGRVDLSELEAICQELVAANPKIVA
                     DVKSGKQQAVGALLGQAKKRNPNANPNDVRRICLELIERMP"
     gene            complement(1818..2249)
                     /locus_tag="VFV87_15675"
     CDS             complement(1818..2249)
                     /locus_tag="VFV87_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105259.1"
                     /translation="MADKSPRRWLQFRLQSVLWLMLCIALAFSAYRYGYRDGFEDQAN
                     QRSQVGGTYAKVYRVGDAVRMVKTKSGDVPDMDGLVRDVTANVLPNTWDTKGGGASIA
                     GFALQPTVSLVVSHDQDGHERITAYLERLRRKKSNQLPTSK"
BASE COUNT          394 a          725 c          784 g          518 t
ORIGIN      
        1 ggtcgagttt tgcctcggct tgacccgcct cttgcaccgg gtgtcttcct tagggcctga
       61 gtatgtcgcc ttcgtacgcc accgccttag agccgtttgt cgatcgccgc aactacgaga
      121 accaggccgg tccgccccag cgggagcggc ggcagttcac caacagccac gagggacttt
      181 cgcccgaggc ccgcgacctc gcccaggcca tcgacagcta caagctgatg caccgccggc
      241 ggttcatcac ctacgaggaa atgctggccg tcgtgaagtc gctgggctat cggagggact
      301 gaacttttcg tcggccctag acaatgccca gggtgatcaa ggcattcttt cgatcagctc
      361 gaggcaaatc cgccttacgt cgttggggtt ggcgttgggg ttgcgctttt tggcctggcc
      421 gaggagggcg ccgacggctt gttgcttgcc ggatttcacg tcggcgacga tcttcggatt
      481 ggcggcgaca agctcctggc agatcgcttc cagctccgaa agatcgaccc ttccgatgcc
      541 caaggcctgc atcgactggg cggcggagcg cttgttcgcg accatgtcgt tgaacacttc
      601 tttggcccgc gtgacttcga tttcgccggc cacgacgagc ttgaccagct cggccagggc
      661 ggcagcggtg agcgggaatt gctcgaggtt ccagccctgt tcgttgagcg ttcgcagcac
      721 ttcctgcgtg acccagttgg ccgccttctt gccgtcgccg ctggcggtgg cgaccgcttc
      781 gtagtaatcg accagcggcc ggccctgggc gacgatcacg tcgctgtcgt agggcgtgat
      841 gccataggcc tgctccagcc gctggcggat gttccgcggc agctcggtca gggagccgcg
      901 gacctgctcg acttcggctg gcggcgtgac gacggggcag aggtccggat cgggaaaata
      961 gcggtagtcg ctcgattctt ccttctcgcg ctggctgaac gtgatgccct tcgcgtcgtc
     1021 ccagccgcgg gtttgcttgg gggcgtcttt gatggtgcgg gccgtttcct gccaggcctg
     1081 gtactggcgg ctggcttcgt aggcgagggc ccgctcgatg tggcggaagc tgttgaggtt
     1141 cttgatctcg acgatcggcg tggcgatttt gccttgcggc gtgtcgatgt ggatgttgat
     1201 gttcgcatcg acccgcagcg agccctcctg catgttgcag tccgaaacgg cgaggtacgt
     1261 gagcagcagc ttcagctcgt tcagataggc ccgcgtctcc tccgtgctgc ggatatccgg
     1321 cgccgagacg atctccagga gcggcgtgcc cgtgcggttc aggtcgatcc gcgtgtcggc
     1381 cttgcccgcc gcctcgtcgt ggaggctttt gccggcgtct tcctccaagt gggcccggat
     1441 gatgccgact ttcttcggct caaagcggct tttggggtcg tgaatttcca gccagccgtg
     1501 ctgcgacatc ggcaggtcgt actggctgat ctggtagccc ttgggcaggt cggggtagta
     1561 gtactgcttg cggtcccact tggtgaacgc ggggattccg cagttgaggg cgatcgccgc
     1621 gaccatgccc aggcgatgcg cctcgcgatt catcaccggc aggctccccg gcaagccgat
     1681 gcacaccggg caggtctgcg tgttcggcgg ggcgccaaac ttcgtgctgc accgacagaa
     1741 gagtttggtg tgcgtcagca actggacatg cgtttccagg ccgacgatga gttggtaggg
     1801 gtggtcgggc atgggggcta tttggaggtc ggaagttgat tcgatttctt tcggcgcaat
     1861 cgctctagat aggcagtgat tcgttcgtgg ccgtcctggt cgtggctgac cacgagggag
     1921 actgtgggct gcaatgcgaa gcctgcgatc gacgctcctc cgcccttcgt gtcccaagta
     1981 ttgggaagca cattggcggt gacgtcgcgt acaagaccat ccatgtcagg cacgtcgccc
     2041 gatttcgttt tcaccattcg caccgcatcg ccaacacgat ataccttggc gtacgttcct
     2101 ccgacctggc tgcgctggtt cgcctgatct tcgaatccat cgcgatatcc gtaccgatag
     2161 gcgctaaagg caagggcgat gcagagcatc agccagagca cggactggag gcgaaactgc
     2221 aaccaccttc gcggagattt gtcggccatg acgtggaccc cggttagagc acaaattcaa
     2281 actgatcaaa cgggcggaaa tcgccgcgat cgagcgcagc caacttcgac acgccatgaa
     2341 ccgtgtacgt ggccgccaac atggtatcga gaacgcgatt gcgtccgagc cgatgatccg
     2401 tcatccatgc cagaaacagc g
//
LOCUS       DASUGB010000828        11479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_786194, whole genome shotgun sequence.
ACCESSION   DASUGB010000828 DASUGB010000000
VERSION     DASUGB010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786194"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFV87_15680"
     CDS             <1..490
                     /locus_tag="VFV87_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105260.1"
                     /translation="AYGGTALDEAEKLIKQMRRQFPQQAEGEREFLDRAAAEIRFHKA
                     ERLMFLARYYDRRAEYRAATHYYARTASEFDDTPHAEEANTRIAAITGLPAVPPQKMQ
                     WLVNLFPESDDVKPLLDASQEIEARDGAQLAAEQNGEIEAQQAANPDAGSGAERAIPL
                     FR"
     gene            498..1067
                     /gene="lptE"
                     /locus_tag="VFV87_15685"
     CDS             498..1067
                     /gene="lptE"
                     /locus_tag="VFV87_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX5105261.1"
                     /translation="MQNSEFRIQIERFLMVRRVVSAFCILHSAFLLTGCAGYQIGQRS
                     LYRPDIRTVHVPVVQSNSFRRYLGERLSEAIVKEIELKTPYKVVGPDAADSVLRVQLV
                     SESKRVLTENRYDEPRDIETDFFIQLSWIDRRGDLISAADIPAQPLLLSISQSASFVP
                     EAGQSISTAQQEAIQRLAEQVVGQMEIAW"
     gene            1127..2005
                     /locus_tag="VFV87_15690"
     CDS             1127..2005
                     /locus_tag="VFV87_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105262.1"
                     /translation="MTTDIPDLDAFYGDLTPARAYVYARLPRPADAEGLTLSGQVRGP
                     RCKLAQTLPTTSPLVDLGPGPTLLARALVPDPVFWSPDLPAIYDVTVNLLRGTEILAT
                     AQRQIGLRSLGVRDRDLLLNGKRWVLRGVSADSTTSRNVTDWHDAAAVYVHDDSPVAL
                     RVASESGVLAMSVVLGEHLNWRIRLLANYPAVAICVPDRLPADFRKAQAVPNLILAQR
                     LSRDATLTRQPWADVVLVTADDTDLVRQAVASIDAPIIAARYRRPPADIATARAACDA
                     LQRDLAPLGQFAGYVV"
     gene            2146..3201
                     /locus_tag="VFV87_15695"
     CDS             2146..3201
                     /locus_tag="VFV87_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5105263.1"
                     /translation="MAPNPPSPLDPQTSRYARQMRFAPLGRAGQEKLLAARALVVGCG
                     ALGAVIANTLARAGVGHLRIVDRDFLELNNLQRQVLYDEDDVAAGLPKAIAAKNRLAK
                     INSEIEIDAVVADVDHTNIERLLDGVDCLIDGTDNFETRFLLNDAAVKLGIPWVYGGC
                     LGAEGQSLTILPGETPCLRCLMPEAPPPGSTPTCDSAGILATIIGVVASLESNEALKI
                     LSGHPDAISRTWTVLDLWDNTLRQIKLEGAKVADCPCCGKREFPWLAGERGSHTAILC
                     GRNAVQLSFPGREPISLDDLAAKLATVGQVTRNKYLVRATIGERLLTVFPDGRAVIGG
                     TEDIAEAKSVYAKFVGN"
     gene            complement(3277..4152)
                     /locus_tag="VFV87_15700"
     CDS             complement(3277..4152)
                     /locus_tag="VFV87_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5105264.1"
                     /translation="MFIRWQLALIASLVLAPALLAADPKTEFLWPKGAPGALGEEAKD
                     KPTLIIYLPEKPNGCGIVVCPGGGYGGLAMDHEGHQIGQWLNENGIAAFICDYRHRGK
                     GYGHPAPLQDAQRAIRTVRARAEEFGVKPDKIGILGFSAGGHLTSTAVTHFDSGDASS
                     DDPVMKVSSRPDFGVLCYPVIAFEQPFTHKGSQKNLLGENAAPELVKSLSNEQQVTDQ
                     TPPCFLWHTYEDTGVPPQNSVVFYRALLDHKVPSELHVYEKGRHGVGLAKDIPGTSAW
                     PGACMAWLKGRGMIQ"
     gene            4236..5111
                     /locus_tag="VFV87_15705"
     CDS             4236..5111
                     /locus_tag="VFV87_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5105265.1"
                     /translation="MTTDLSQRHELVGDLFQLPQSPAEWERYRLSDEQVAFYHDQGYL
                     AGVRILTVEQIEKLRGELAAFFNPQHPGHELWYEYHTNESARPDTVLFHALGAWRITP
                     GFHDILWHPAFTMAASQLLGGAVRFWHDQLFCKPAKHGGVVAWHQDYSYWTRTRPMQH
                     LTCWIGLDESSRDNGCVHYVPGSHRWDLLPITGLAGNMDAIQEVLSPAQWEQFTHPVA
                     IELRAGECSFHHPLMVHGSFENRTDRPRRATVINAFKDGTQSDSDQPLLTGVPPVPKG
                     EQMGGQFFPLLFPCP"
     gene            5217..6230
                     /locus_tag="VFV87_15710"
     CDS             5217..6230
                     /locus_tag="VFV87_15710"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX5105266.1"
                     /translation="MFTAETPSYAIDLDLPEPERWREVIAAEQANARRLIDRAAADLE
                     RVPRTARWTFGQLYRMFGGLYQGEIAAWAGALGVSVGTATMLNCLYELSHLPQPKWLG
                     CTAGIRWLEGHGMVHVRTLDWPIDGMGEATRLFRFRRGAREFVSVAMPGQVGMLSGML
                     PGAYSVTINWAPPAALPNFDFGPGFLLRDVFETCDTYAAAVERLERERLSTSVFYTVC
                     GAERGQACVIERTQAGAAVRRFADGPLVQANHHIAQAFSANNEAIREVPPEEEVFSID
                     GSSKRVKIMQDGLAALPATCSLEAATAPLDVPTVFNSQTCQKMVFCPATGELKLWRLV
                     SEA"
     gene            6247..8553
                     /locus_tag="VFV87_15715"
     CDS             6247..8553
                     /locus_tag="VFV87_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5105267.1"
                     /translation="MQIIKRMAFSDLAVTIVLVLFAAPAASDDQWRKLPSLPGRADHI
                     SAIALEGDTLWATAGTAVAYWDGGAWQIPDGPKLLGGIYLSQFMGGGERPLYLTQPGE
                     TEGRGRIFRLSDGAAKAEADFEYEAAHMSPGIMATRAGFIRLAKGQLSSCAKRVWTKV
                     AAPEEFPLVIETADATCCYFPRAQKLLRIDLAGKIREERVALAFSKNGTGIRGRRWGE
                     DRVLLLEQFRPNLTGFRLATGEALDLGELNGKIDESALWDCISTRDGSVWLLVTNAKT
                     EKREVIRVRASGECETLADIPRTRWDQNSFSQHARAVLETKTGDLWLGGGLELLRIRE
                     GSGSLFGQETGLALHATTHLLEDSRGNLFAANSGGVYARFAEEPPAALPAPRKPDPSA
                     PPVWTFPREKLPPLKAAWKVGPNLYAITGHLTVIQKPEQRELCWLVAVAADTGERRFQ
                     RELVSDVFATPWLLEQSDEKILQIALPKRLGRFDAQTGKTLAALDLACDSRIAPLPVE
                     GGHVVIPTSRGSEAALVDQEGRTKWSCTLPGYVMLHPASFGQLALFQTRGGSYGGQET
                     ACVNLEKGTLLWTDVTDAYGCGAAFFNDEAFVVEADQFFSPQRSEGWLIGRNPQTGER
                     LWHYRQAGGIHHPPLVDRRTARVYGVFSRGEVVCLRGEDGSVVWQQTLPEGASAAGAA
                     DSYHPAWSPHTLAGGRLAVVDRNLVLHLLDAKTGEFQASIALHFDDPPMGIPAPQLVG
                     PPWISDDAVIAAFSTGLVSIPIAKKAQP"
     gene            complement(8570..9829)
                     /locus_tag="VFV87_15720"
     CDS             complement(8570..9829)
                     /locus_tag="VFV87_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105268.1"
                     /translation="MRYFRQFVSLLQAAVLLALCGPLRGEIFVLKSGGRVEGELLNPQ
                     REPGALYQLRTDEGLRLALGEAQVHRVIVKSDAEKEYEAALLATGNTVEEQWAMAEWC
                     KDVGLLAQRKRHLAEVIRLEPDHKEARAALGYSLFAGKWMTQDDYMTGRGYVRYKGGW
                     TTRQQMEIDIREDKQEVDAKDWRKQIRIWLDQLGTKRHELATAGLAQIRDVTAVPALA
                     EILADRDQPRANRIICLEILGKLPPGLASGALVKLAMDERDEDLRDRVLDELIRQGPH
                     TVLGALLRELKNERETPFSKNARINRAAYCLQRLGDTDATLPLIEALVTDHYVIVDPS
                     AGGGGGMPINFNAGGPVGPGGQGGLGGLSMGGKPKKVKGQVKNQYVLGALTSMYPGVN
                     YLYDVAEWKRWYIDTQTSTHIDLRRDE"
     gene            complement(10016..10549)
                     /locus_tag="VFV87_15725"
     CDS             complement(10016..10549)
                     /locus_tag="VFV87_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105269.1"
                     /translation="MDHPDPLFPLLLLLRYMHILGAIALMGGAIFMRFALAPTVITLE
                     GSTRAEVHERVRARWSKFVMAAAGLLLVSGLANMMLYSSRRYDMSPVPIVNASYAMFV
                     GIKFLLALPIFLFASFLAGKSATAKKFQEKAVFWMNVNLTLALVMVLMGGLLKFVQRE
                     YKEQPAAPQAASAALLP"
     gene            10795..>11479
                     /locus_tag="VFV87_15730"
     CDS             10795..>11479
                     /locus_tag="VFV87_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105270.1"
                     /translation="MRSFVAATCCLIVGLEILIGVPVAICLGFLCLGAGAAPGVYVSE
                     AQFDAPTCPPPIYSSPVYSPGPVPCGPYDPSYPPSSQSPSPMKPSVCPVPMAPGVVAS
                     LPASDSAADPLPHCTSNSTVSEYPIAATSSADGLQPQLFAHVCLEGDCPAGQSDRADS
                     TRKSSPESALAEALLATATLLYQQADGHEIEQEYALADRLRALARDLRDEASSIGRRS
                     PDYDFGPVSD"
BASE COUNT         1952 a         3828 c         3654 g         2045 t
ORIGIN      
        1 ggcatatggc ggtacggcct tggacgaggc ggaaaagctc atcaagcaga tgcgccgcca
       61 atttccccag caggccgagg gcgagcgcga gttcctcgac cgggccgcag ccgagattcg
      121 cttccacaag gccgaacggc tgatgttttt ggcccgctat tacgatcgcc gggcggaata
      181 ccgggcggcc acccactact acgcccggac ggccagcgag ttcgacgaca cgccgcacgc
      241 tgaagaggcg aacacgcgaa tcgccgcgat caccggcctg cccgccgttc cgccgcagaa
      301 gatgcagtgg ctcgtcaacc tgttccccga aagcgacgat gtcaagccgc tcctggatgc
      361 gtcgcaggaa atcgaggctc gcgacggcgc tcaactggct gccgagcaga acggcgaaat
      421 cgaagcccag caagccgcca acccggacgc cggttccggc gccgagcggg cgattccgct
      481 gtttcggtga tcttggaatg cagaattcag aattcagaat tcagattgag cgattcctga
      541 tggttcgccg cgtcgtttct gcattctgca ttctgcattc tgcatttctc ctcaccggct
      601 gcgccggcta tcagatcggc caacgctcgc tctatcggcc ggatatccgc accgttcacg
      661 tgccggtggt gcagtcgaac agcttccgcc gctatctggg cgaacggctg agcgaggcga
      721 tcgtcaagga aatcgagctg aagacgccgt acaaagtcgt cggccccgat gccgccgaca
      781 gcgtcctccg cgtgcagctc gtctcggaga gcaaacgcgt actgaccgag aaccgctacg
      841 acgagccgcg cgacatcgag accgatttct tcatccagct ctcctggatc gaccgccgcg
      901 gcgacttgat cagcgccgcc gacatccccg cccagccgct cttgctcagc atcagccaat
      961 ccgccagctt cgtccccgaa gccggccaat caatcagcac cgcccagcag gaagcgatcc
     1021 agcgcctggc cgagcaggtg gtggggcaga tggagattgc gtggtaggtc agtggtccgt
     1081 tgtcagttgt ccgtcggggg atacgatacc cactgacaac tgaccaatga caacggacat
     1141 tcccgatctc gacgccttct acggcgacct cacgccggcc cgcgcgtatg tctatgcccg
     1201 gctgccgcgg ccggcggatg ctgaggggct gacgctgagc ggccaggtgc gcgggccgcg
     1261 gtgcaaactc gcccagacct tgccaacgac ttcgccattg gtcgatctcg gccccgggcc
     1321 aacgctcctc gcgcgggcgc tcgttcccga tcccgtcttc tggtcgcccg atctgccggc
     1381 gatttacgac gtgaccgtca acctgctccg aggcacggaa attctcgcca ccgcccagcg
     1441 ccagatcggc ctccgctcgc tcggcgtccg cgaccgcgac ctccttctga acggcaaacg
     1501 ctgggtgctg cggggggttt cagcggattc gacgacgtcg agaaatgtaa ccgactggca
     1561 tgacgccgct gccgtgtacg tccatgacga ttcgccagtc gctttgcgag tcgcatcgga
     1621 gtcgggagtc ctcgcgatga gcgtcgtttt gggtgagcac ttgaattggc gcatccgact
     1681 cctagccaac tacccggccg tcgctatttg tgtgcctgac agattgcccg cagattttcg
     1741 taaggcgcag gcggtgccga atctgattct cgcacaacgc ctaagccgcg atgcaacgct
     1801 cacgcggcag ccctgggcgg acgtagtatt ggtaaccgcg gacgacacgg atctcgtgcg
     1861 ccaggcggtc gcgtcgatcg acgctccgat catcgccgcc cgctaccgcc ggccgccggc
     1921 cgatattgcc accgcccgcg cggcctgcga cgcactgcag cgcgaccttg cacccctcgg
     1981 ccaattcgcc ggctacgtcg tatagactga ataccaaccc gaagcgtaag cgagggagtg
     2041 aagcccgagc gaccgagtga attcagcgcg actgctgact ctggcctgac tccctcgctt
     2101 acgcttcggg ttagtgtgtt cgatccgtgt catctgtgtt catccgtggc tccaaatccc
     2161 ccaagcccgc tcgacccgca aaccagccgc tatgcccggc agatgcgctt cgcgcccctg
     2221 gggcgcgcag ggcaagagaa gctgctggct gcgcgggcgc tggtggtcgg ctgcggggcg
     2281 ctcggagcgg tgatcgccaa tacgctggcc cgggccggcg tcggccacct gcggatcgtc
     2341 gatcgcgatt tcctggagct caacaacctg cagcggcagg tgctctacga cgaggacgac
     2401 gtcgccgccg gcttgcccaa ggcgattgcc gccaagaatc gcctcgcgaa gatcaattcc
     2461 gaaatcgaga tcgacgcggt cgtcgccgac gtcgatcaca cgaacatcga acgcctgctc
     2521 gatggcgtgg attgcctgat cgacggcacc gacaatttcg agacgcggtt tctgctcaac
     2581 gacgcggcgg tcaagctcgg cattccgtgg gtctatggcg ggtgcctggg cgcggaaggc
     2641 cagtcgctga cgatcctgcc gggcgaaacg ccctgcctcc gctgcttgat gcccgaagcc
     2701 ccgccgccgg gcagcacgcc gacttgcgac agcgccggca tcctggccac gatcatcggc
     2761 gtcgtcgcct cgctggaatc caacgaagcc ctgaaaatcc tcagcggcca cccggacgcc
     2821 atcagccgca cgtggaccgt cctcgacctc tgggacaaca cgctgcggca gatcaagctg
     2881 gagggggcca aggtcgcgga ttgcccgtgc tgcgggaagc gagagttccc ctggctggcg
     2941 ggcgagcgcg gcagccacac ggccatcctc tgcggccgca acgccgtgca gctcagcttc
     3001 cccggccgcg agccaatctc gctcgacgac ctggccgcca agctcgccac cgtcggccag
     3061 gtcacgcgca acaagtacct cgtgcgggcc acgatcggcg agcgcctgct caccgtcttc
     3121 cccgacggcc gcgcggtcat cggcggcacc gaggacatcg ccgaagccaa aagcgtgtac
     3181 gcgaagttcg tcgggaatta gatttcgccg cagccgcgtt cgccagaacg cgggcaaccc
     3241 gggcgacccg cactctggct agtgcggcta ccagatttat tggatcattc cgcgaccctt
     3301 cagccacgcc atacacgcgc cgggccaggc gctcgtgccg ggaatgtcct tggccaggcc
     3361 gacgccgtgg cggcccttct cataaacgtg cagttcggat gggaccttgt ggtcgagcag
     3421 ggcccggtaa aacacgacgc tgttctgcgg cggcacaccg gtgtcttcat acgtgtgcca
     3481 caggaagcac ggcggcgtct ggtcggtcac ttgctgctcg ttcgagaggc tcttcaccag
     3541 ctccggtgcc gcattctcgc ccagcaaatt cttctgcgag cccttgtgcg tgaacggctg
     3601 ctcgaaagcg atcaccggat agcagagcac gccgaaatcg ggtcggctgc tgactttcat
     3661 cacggggtcg tcgctgctcg catcgccgga atcgaagtgc gtgaccgcgg tgctcgtcag
     3721 atgcccgccc gccgagaagc ccaggatgcc gatcttgtcc ggcttcacgc cgaactcctc
     3781 ggcccgcgcg cgcaccgtgc ggatcgcccg ctgagcatcc tgcagcgggg cgggatggcc
     3841 atagcccttg ccgcggtggc gatagtcgca gatgaacgcc gcgatgccgt tctcgttcag
     3901 ccactggccg atctggtgcc cttcgtggtc catcgccagc ccgccatagc caccccccgg
     3961 gcaaaccacg atcccgcagc cgttgggctt ctccggcaga tagatgatca gcgtcggctt
     4021 gtccttggct tcttcgccca gcgctcccgg cgcgcccttc ggccaaagga attctgtctt
     4081 cggatcggcg gccaaaagcg ccggagcgag aacgagcgag gcgatcagtg ccaattgcca
     4141 gcgaatgaac atggtcgtaa agtctcgtta gaggatagcg atcgcgattt gcctatgcta
     4201 actgcgccgc ctgcgctgct caattgagga ccacgatgac aacggacctt tcccagcgcc
     4261 acgagcttgt gggcgatttg ttccagctcc cgcagtcacc cgcggaatgg gaacgctatc
     4321 gcctgagcga cgagcaagtg gctttctatc acgaccaggg ctacctggcc ggagtccgca
     4381 tcctcactgt ggagcagatc gagaagctcc gtggcgaatt ggccgcgttc ttcaaccccc
     4441 agcatcccgg ccacgagctg tggtacgagt atcacaccaa cgaatcggcc aggcccgaca
     4501 cggtcctctt tcacgccctc ggcgcgtggc ggatcacgcc cggctttcac gacatcctct
     4561 ggcacccagc cttcacgatg gccgccagcc agctcctcgg cggggcggtc cgtttctggc
     4621 acgaccagct cttctgcaag ccggcgaagc atggcggcgt cgtcgcctgg caccaggact
     4681 attcgtactg gacccgcacc aggccgatgc agcacctcac ctgctggatc ggcctcgacg
     4741 aaagcagcag ggacaacggc tgcgttcact atgtgcccgg cagccaccgc tgggatctgc
     4801 tgccgatcac cggcctggcg ggcaacatgg acgcgatcca ggaggtgctc agccccgcgc
     4861 agtgggagca gttcacgcac ccggtcgcca tcgagctccg ggccggcgag tgctcgttcc
     4921 accatccgct gatggtccac ggcagcttcg agaaccgcac cgaccgcccg cggcgggcca
     4981 ccgtcatcaa cgccttcaag gacggcaccc aaagcgacag cgaccagccc ctcctcaccg
     5041 gcgtgccgcc tgttcccaaa ggcgagcaga tgggcggcca attcttcccg ctgctatttc
     5101 cctgcccgta ggctggtccc caggcgagct tttccagttt cccggcagac ccgcctcatc
     5161 ctgggcgttg atcggtgtaa attagcgttg ctactccctc ccggaactaa accgcgatgt
     5221 tcaccgccga aacaccttcc tatgcgatcg atctcgatct ccccgagccg gagcggtggc
     5281 gggaggtgat cgcggcggag caggcgaatg cccggcggct gatcgaccgg gcggcggccg
     5341 atttggagcg cgtgccgcgg acggctcgct ggactttcgg gcagctgtac cggatgttcg
     5401 gcggcctgta tcagggggag attgcggcct gggccggggc actgggagtg agcgtcggca
     5461 cggcgacgat gctcaactgt ctgtatgagc tgagccatct gccgcagccc aagtggctcg
     5521 gctgcacggc ggggatccgc tggctcgaag gacacgggat ggtccacgtc cgcacgctcg
     5581 actggccgat cgacggcatg ggcgaagcga cgcggctgtt ccgctttcgc cgtggggcgc
     5641 gggaattcgt ctcagtcgcg atgccggggc aggtgggcat gctcagcggc atgctgccgg
     5701 gcgcctattc ggtgacgatc aactgggccc cccccgccgc gctgccgaac tttgactttg
     5761 gcccgggctt tttgctgcgc gacgtgttcg agacgtgcga tacctatgcg gcggccgtcg
     5821 agcgcctgga gcgggagcgg ctttcaacga gcgtgtttta cacggtttgc ggggcggagc
     5881 gggggcaggc gtgcgtcatc gagcggacgc aagccggcgc ggcggtccgg cgattcgcgg
     5941 atgggccgct ggtgcaggcc aatcaccaca ttgcccaggc gttttccgcg aacaacgaag
     6001 cgattcgcga agtcccgccg gaggaagagg tcttttcgat cgacggcagc agcaagcgcg
     6061 tcaaaatcat gcaggacggc ctcgccgcgc tccccgcgac ctgctcgctc gaagcggcga
     6121 ccgctccgct cgatgttcct acggtcttca actcgcaaac ctgccagaaa atggtcttct
     6181 gtcccgccac cggcgagctc aaactctggc ggctcgtttc ggaggcgtag tcgttggagt
     6241 gcagcgatgc agatcatcaa gcggatggcc ttcagtgatc tggcggtgac gatcgtgctg
     6301 gtgctcttcg ccgcgccggc cgccagcgac gaccagtggc gcaagctccc gtcgcttccc
     6361 ggccgggccg accacatttc ggcaattgcc ctggaaggcg acacgctgtg ggccacggcc
     6421 ggaactgccg ttgcgtactg ggacggcggc gcgtggcaaa tcccggacgg accgaagctc
     6481 ctcggcggaa tttacctgtc gcaattcatg ggcggcggag agcggccgct ctatctcacg
     6541 cagcccggcg agacggaggg acgcggccgc attttccggc tcagcgatgg ggcggccaag
     6601 gcggaggcag actttgagta cgaagcggca cacatgtcgc cgggcatcat ggcgacgcgc
     6661 gccggcttca tcaggctcgc taaaggtcag ctctcgtcgt gcgcaaagag ggtctggacg
     6721 aaggttgccg cacccgagga atttccgctg gtcatcgaaa cggcggatgc cacctgttgc
     6781 tattttccgc gtgcccagaa gctgctccgc atcgatctcg cgggcaagat ccgcgaggag
     6841 cgggtcgccc tcgcgttttc gaagaacggc acgggcatcc gcggccggcg ctggggcgaa
     6901 gaccgcgttt tgctgctgga gcagtttcgg ccgaacctga ccggctttcg cctggcgacc
     6961 ggcgaagcgc tcgatctggg cgaattaaac ggcaagattg acgagagcgc tctttgggac
     7021 tgtatctcca cgcgcgatgg cagcgtctgg ctgctcgtga ccaatgccaa gacggaaaag
     7081 cgggaggtca ttcgcgtccg cgcgtcgggc gaatgcgaaa cgctggctga cataccgcga
     7141 acgcgctggg atcagaattc ctttagccaa catgcgcggg ccgtcctgga aaccaagact
     7201 ggcgacttgt ggctgggtgg cggccttgag ctgctccgca tccgagaggg gagtgggagc
     7261 ctgttcgggc aagaaaccgg cctggccctg cacgccacga cgcatttgct ggaggattcc
     7321 cgcgggaatc ttttcgcggc caactcgggc ggggtatatg cacgatttgc cgaggagcct
     7381 ccggccgcgc tcccggcccc gcgcaagccc gatccgagcg ccccgcccgt ctggacgttt
     7441 cctcgcgaga agcttccgcc cctgaaggcg gcttggaagg tcggccccaa cctctatgcg
     7501 atcacgggcc acctcacagt catccagaag cccgagcaac gggagctgtg ctggctcgtg
     7561 gcggttgcgg cggacaccgg tgagcgccgt tttcagcgcg agctggtgtc tgacgtcttc
     7621 gcgacgccgt ggctcctcga gcagtccgac gaaaagatcc tgcagatcgc cctgcccaag
     7681 cggctgggcc gcttcgatgc tcagacgggg aaaactctcg cggcgctgga cctcgcctgc
     7741 gacagccgca tcgctccgct gcccgtcgag gggggccatg tagtaattcc gacttcgcgc
     7801 ggcagtgagg cggcgctcgt ggaccaggag ggcaggacca aatggagctg cacgctgccc
     7861 ggatatgtca tgctgcatcc cgcatcgttc ggccagctcg ccctgttcca gacccgcggc
     7921 ggcagctacg gcgggcaaga gacggcgtgc gtcaatctgg agaaggggac gctcctgtgg
     7981 accgatgtga ccgacgctta cggctgcggg gcggcgtttt tcaacgacga ggccttcgtc
     8041 gtcgaagcgg accagttctt ttcgccccag cgcagcgagg gctggctgat cgggcgcaat
     8101 ccgcagaccg gcgagaggct gtggcactat cgccaggcgg gaggcatcca ccatccgccg
     8161 ctggtcgatc ggcgcacggc ccgcgtgtac ggcgtatttt cccgcggcga agtggtttgc
     8221 ctgcgcggag aggacggcag cgtcgtttgg cagcagacgc tgcccgaagg cgcgtctgcg
     8281 gcgggagcgg ccgattccta ccatccggcc tggtcgccgc acacgctggc gggcggccga
     8341 ctggcggtcg tcgatcgcaa tttggtgctg cacctgctcg acgccaagac gggcgagttc
     8401 caagcgagca tcgccctgca cttcgacgat ccgccgatgg gaatcccagc gccgcaactg
     8461 gtcggacctc cgtggatttc cgacgatgcg gtgatcgctg cgttttcgac ggggctcgtc
     8521 tcgattccca tcgcgaagaa agctcagccc tgaagagcac agagaatgcc tactcgtccc
     8581 gccgcaggtc gatatgggtg ctggtctgcg tgtcgatgta ccagcgtttc cattccgcga
     8641 cgtcgtagag atagttcacg ccgggataca tgctggtcag agcgcccagg acgtactggt
     8701 tcttcacctg gcccttcacc ttcttgggct tgccccccat gctcaacccg ccgaggcccc
     8761 cctggccgcc cggtcccacg gggccgcccg cattgaagtt gatcggcatg ccgccgccac
     8821 cgcccgcgct cggatcgaca atgacgtagt ggtcggtgac cagggcctcg atcagcggga
     8881 gcgtggcgtc ggtgtctccc agccgctgca ggcagtaggc cgcccggtta atgcgggcgt
     8941 tcttgctgaa gggcgtttcc cgctcgttct tcaattcgcg gagcagcgcg ccgaggacgg
     9001 tgtgcggtcc ctggcgaatc agttcatcga gaacgcggtc gcggaggtct tcgtcgcgct
     9061 cgtccatcgc cagcttcacc agcgccccgc tggccaggcc cggcggcagc ttgcccagga
     9121 tctccaggca gatgattcga ttggcccgcg gctgatcgcg gtcggccaga atttccgcca
     9181 gtgccgggac agcggtcacg tcgcgaatct gtgccaggcc ggcggtagcc agctcgtgcc
     9241 gcttggtgcc gagctggtcg agccagatgc gaatctgctt ccgccaatcc ttcgcatcca
     9301 cctcttgctt gtcctcacgg atgtcgattt ccatctgctg ccgcgtggtc cagccccctt
     9361 tgtagcgcac gtacccgcgg ccggtcatgt agtcgtcctg cgtcatccac ttgccggcga
     9421 acaggctgta gcccagggcc gcgcgggcct ccttgtggtc gggctccagg cggatgacct
     9481 cggccaggtg ccgcttacgc tgggccagca gcccgacgtc cttgcaccac tcggccatcg
     9541 cccattgctc ctccaccgta ttgccggtgg cgagcagggc ggcttcgtat tccttttcgg
     9601 cgtcggactt gacgatcacg cgatggacct gggcttcgcc cagcgcaagg cgcaggcctt
     9661 cgtcagtccg cagttgatag agggcgccgg gctcccgctg cggattgagc aactcgccct
     9721 cgacccggcc gccggatttg aggacgaaga tctcgccgcg aagtgggccg cagagcgcca
     9781 gcaggaccgc tgcctggagt agagagacga attggcgaaa atagcgcatg gtcgcagacc
     9841 cggttgctgc cgctcgcccc tgatgcccgc ccggcaagtc tccgggcaaa ccttcgccca
     9901 ttataggccg cgaaacgggg gcaggcgacc ggaatccggc gtggggcagg cattcttgcc
     9961 tgccgttgcc ctgcacttcc aagggcagac aagaatgtct gccccacttg ctcccttacg
    10021 gcagcaatgc cgcggaggct gcttggggcg ctgcaggctg ctctttgtat tcgcgctgga
    10081 cgaacttcaa gaggccgccc atgaggacca tgaccagggc taaggtcagg ttgacgttca
    10141 tccagaagac agccttttcc tgaaactttt tggccgtcgc actcttgccg gccaggaagc
    10201 tggcgaacag gaagatcggc agcgccagca ggaacttgat gccgacgaac atggcgtagc
    10261 tggcgttgac gattggaaca ggcgacatgt cgtaccgccg gctggaatag agcatcatgt
    10321 tggccaggcc gctcaccagc agcagccccg ccgcggccat gacgaacttt gaccaccgcg
    10381 cgcggacgcg ctcgtgaact tcggcccgcg tgctcccttc gagcgtaatt accgtcggcg
    10441 cgagggcgaa ccgcatgaag atcgcgcccc ccatgagcgc gatcgcgccg aggatatgca
    10501 tatagcggag cagcaaaaga agcgggaaga gggggtcggg gtgatccatg agaggacgga
    10561 ggacagaaga cagcggacag agacggcagt caggcccatc atggtagtcg gtgaggcgtc
    10621 aatctccaac gtacccgcat tgctagcgat aacactggcc cctttcctgt cctctgacct
    10681 ctgtcctctg tcctcttgtt tccaactgcc cttgggaccg cgccccgccg cggcctatac
    10741 tgctcgtccc gcttgattcc cgcaatgcat gatccacaag acaaggaggt cctcatgcgc
    10801 agtttcgttg cggcgacttg ctgcttgatt gttggcttgg agattttgat cggcgtgccg
    10861 gtcgcaattt gcctgggatt cctctgtctc ggcgcgggcg cggcgccggg cgtgtacgtc
    10921 tcggaggccc agttcgacgc gccgacgtgc ccgccgccga tttactcctc cccggtctat
    10981 tcgccagggc cggtcccctg cggaccctac gatccgtcat atccgccgtc gtcacaatcg
    11041 ccatcgccaa tgaagccgtc ggtttgtccg gtgccaatgg cgcctggcgt ggtggcgtcg
    11101 ctgcccgctt ccgattcagc cgccgacccg ctgccgcact gcacttccaa ctcgacggta
    11161 tcggaatacc cgatcgccgc cacgagcagc gccgacggcc ttcagccgca gctcttcgcc
    11221 cacgtatgcc tggagggaga ttgcccggct ggacaatccg accgtgctga ttcgacgcgg
    11281 aagtcctcgc cagagtcggc tctcgcggag gcgctgttgg cgaccgcgac gttgctctac
    11341 cagcaagccg acgggcacga gatcgagcag gaatatgccc tggccgatcg gctgcgcgcg
    11401 ctggcccgcg acctgcgcga cgaagccagc agcattggtc gccgctcgcc ggactatgat
    11461 tttggtcctg tcagcgatc
//
LOCUS       DASUGB010000829         7891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_925529, whole genome shotgun sequence.
ACCESSION   DASUGB010000829 DASUGB010000000
VERSION     DASUGB010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925529"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="VFV87_15735"
     CDS             complement(<1..1231)
                     /locus_tag="VFV87_15735"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5105271.1"
                     /translation="MSRSALALLAVLAWIPAAAAKADPTLPEPTLASPFQAIDLNVGD
                     SQEVSLAGQKIRVKLLDLKEQRDSLRGAVRRGEVRVEVAGQTVTIVSATYHLPVKVGP
                     VQIDCPITGGYTRNSTKTTGGLAPWGLEKDARLRLWPTDAPWIDTETFQYPAKQAWFA
                     SATQMANEPTYVDGGERPGAGNVYYHYGLDIGGAEGLVDVVAATDGLVVSVGKEALPG
                     YEDTPVSPRYDVIYLLDNRGWFYRYSHLHTIAAGLKPGQRVERGQALGILGKEGGSGG
                     WSHLHFDISGRQPSGKWGIIDGYAFLWEANLLEKQPKLIAVARPHHFAAVGEKVRLDG
                     SRSWSAAGPISKFAWTCTDGQTAAGPEFERTYSQPGVYSEILEVTDHEGRIDYDFAIV
                     NIVDREHPEQLPPSIHAA"
     gene            complement(1270..2442)
                     /locus_tag="VFV87_15740"
     CDS             complement(1270..2442)
                     /locus_tag="VFV87_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105272.1"
                     /translation="MPGNNAVGVKNTRCTRRLVLQSLAAGATLVSARPMHFCNADERP
                     EREAARPWISIRGIYGGYPREVLDRGETPANYGINAIWVGSGSLDATEIDRYRKLGLK
                     VFAEFNSLHAAEYLKEHPDAAPIGTDGKPSPPPQGWQGVSAFHRDYRRDRMAEFRRVL
                     QTFEIDGIWLDYHHAHASWERAEPELPDTDFSPAALNQFSEKSGITLPTDTAAAAKVL
                     LGKEREAWTNFRCDVFTDWVREYREILDDVRPKALIGTFHCPWSQDEYEGAIRHKLAI
                     DLKAQAKYLDIFSIMPYHARFGHAEDPAWISRQVQKLGELLKIQGKPDEKNRIWPIAQ
                     LSDWGSRVPFEQVAAVLDHGTRSPATGVMVFHWSGVSKEWSKVKAMQRKYLAFRGS"
     gene            2525..4441
                     /locus_tag="VFV87_15745"
     CDS             2525..4441
                     /locus_tag="VFV87_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105273.1"
                     /translation="MPRLFSIVCLAFACLVAAREVATAGEIQAQEPERSGQTLHQPSN
                     TWTKRSPLADVPVSPRLGYEGACVWDSRHQLLVRYGGHNQGGGGEQGAEVWTWDPITG
                     KWALKEPNTSPPGVCCNAQNIYDPIGGRYIRFPLFSGSHGWQWARELYLNDSSVWTYD
                     VGTNRWRNMRPLPAPRLAPYRCASWDSDEQVVVVFGGEGSHEGTLIYDPARNEWRKPK
                     PAVEPEGRSGGNMAYDAARKVHVLFGSQFTDDPHTWTYDVRKNEWRDMQPPSQPPTDK
                     NDAVLTYDPIQQRVLAIVKITTGKDEEARHELQTWAYDAGANRWERKNPMSEPQPSSN
                     RARQLIFAPELNLAILENCTSRPREQQIWTYRLGDSPAAPAARPLEARSVPPLVEDVV
                     VSVVSPNKVEVAWTAPAGAVASGYHVERAVVEVWSDDQLRRLKQRTTPLAEPAVGAIR
                     RIGPFQRLTKEPIRGTAFTDTEVDLRKPQTIDGDPVWDSSLHAEHLDESGRGYRLGVF
                     AYRIRALDRAGAEFGPSPAVLTIPSSPQHLFSREDGTTCQLKWSPNSEQGIAGYRVYR
                     MDGRYDKEPVSRLTEATLTDTKYEDATAGKSTRRYYVIAVDALGQEGFTSSPVWFQRE
                     WRDFYRPFVAEWHQ"
     gene            complement(4465..4800)
                     /locus_tag="VFV87_15750"
     CDS             complement(4465..4800)
                     /locus_tag="VFV87_15750"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105274.1"
                     /translation="MFGEFGRTLKFNGKAGRDHWSRCMSLALAGGGIRGGAVHAKSGK
                     HATEPIDAYVRPADFLATILHCLGFAPETLYDAQGRPFSLTRDRMIEDTGESSVTRSF
                     VHGRYTPRE"
     gene            5117..6580
                     /locus_tag="VFV87_15755"
     CDS             5117..6580
                     /locus_tag="VFV87_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5105275.1"
                     /translation="MKLLRPVCPEILIGITLLLASGLAAAEPLRPAQQSAVEEIDARQ
                     SELNEVNKAIWDFAEVGLEERRSSALLVEKLKAAGFKVRVGVADMPTAFVAEFGAGHP
                     IIGILAEYDALPGLSQKIAPQRDAVTADAPGHGCGHSGLGTGALGAALAVKAAMEKHS
                     LPGTVRLYGTPAEETLIGKVYMTLAGEFEQLDACLHWHPSDRNEVWNGVSKAAVSAKF
                     TFTGVTAHASGSPDKGRSALDAVELMNVGANYMREHLKEDARVHYVITNGGGAPNVAP
                     AKATVWYYVRANEHADVERNFIWLRDIADGAAKMTRTSVSVQIDTDCHEIIPNEPLAA
                     LLLVNLERVGPPKFTPDEHTFARRLQESVTQEFGTVFDLALDEEVHRTRKLGAGVGST
                     DVGDISWHVPTGGFRAACFASDSPGHSWQNVAAIGSTIGEKGTGYAAKVLACTTLDLL
                     EDPQTLAAAKLDFAERMEGRKYVTLIPPGQKAPKSIR"
     gene            7122..7394
                     /locus_tag="VFV87_15760"
     CDS             7122..7394
                     /locus_tag="VFV87_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105276.1"
                     /translation="MAKRRNKASKIRAVLEELGPDAKVAQVVETLAARRVRVRPQQVY
                     AIKAANKNGRSNAGDYNSLVKAKKLADAMGGVEKAKAALDVLAKLM"
     gene            7430..>7891
                     /locus_tag="VFV87_15765"
     CDS             7430..>7891
                     /locus_tag="VFV87_15765"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5105277.1"
                     /translation="MPGRVTRRELLRTTAAAGLATLAPSLPAAEDRERRTFVVGHRGL
                     LHSAPENTLAAFRGCLALRVGFEFDVRRTNDGQLVCLHDATLDRTTSGTGRLADRTLN
                     ELRRLDAGSWFDDSFHGERVPTVNEVLALVAERATDSTFIAVDLKETGHGID"
BASE COUNT         1498 a         2449 c         2473 g         1471 t
ORIGIN      
        1 aggccgcgtg gattgatggc ggcaactgct cgggatgctc gcgatcgacg atgttgacga
       61 tggcgaaatc ataatcgatc cggccttcgt gatcggtgac ttcgaggatt tcgctataga
      121 cgccgggctg cgagtaggtg cgctcgaatt ctggacccgc tgcggtctga ccgtcggtgc
      181 aggtccaggc gaacttcgag atcggccccg ccgctgacca cgaacggctg ccgtcgagcc
      241 gtactttctc tccgaccgcg gcaaagtggt gcggccgggc gacggcgatc aatttgggtt
      301 gcttttcgag gaggttggcc tcccacaaga aggcatagcc gtcgatgatc ccccacttcc
      361 ccgacggttg gcggccgctg atgtcgaagt gcagatgcga ccagccgccg ctgcctcctt
      421 ctttgccgag gattcccagc gcctgcccac gctccactcg ttgccccggc ttgagcccgg
      481 ccgcgatcgt gtgcaggtgg ctgtagcgat agaaccagcc gcgattgtcg agcagataaa
      541 tgacgtcgta tcgcggagaa acaggcgtat cctcgtagcc gggcaacgcc tccttgccga
      601 cggaaacgac caaaccatcg gtggcggcaa cgacatccac gagcccctcc gcaccgccga
      661 tatccagccc atagtggtaa tagacatttc cggcgccggg cctttcgccg ccatcgacat
      721 aggtcggctc gttcgccatc tgcgtcgcgc tggcgaacca ggcctgcttc gccggatact
      781 gaaacgtttc agtgtcgatc cagggcgcat ccgtgggcca cagccgcagc cgggcatctt
      841 tttccaagcc ccagggcgcc aggccgccgg tggttttcgt cgaattgcgc gtgtagccgc
      901 cggtgatggg acagtcgatt tgcacgggac cgaccttgac tggcaagtga tacgtcgccg
      961 acacgatcgt taccgtctgt ccggcaactt cgacccgcac ttccccccgg cggaccgcgc
     1021 cgcggagcga atcgcgctgc tccttgagat cgagcagctt cacccggatt ttctgccccg
     1081 ccagcgatac ttcctgcgag tcgccgacgt ttagatcgat ggcctgaaac ggtgatgcca
     1141 gcgtcggctc tggcaatgtc ggatcggcct ttgcagcggc tgcagggatc caagccaaaa
     1201 cggcgagcaa ggcaagcgcg gagcgactca tcggttgttc ctcaatttga aggcacacca
     1261 aagttggtac tacgaaccac gaaatgcgag gtatttgcgt tgcatggcct ttaccttgct
     1321 ccactcctta gagacgccac tccagtggaa aaccatgacg ccggtggcgg gcgagcgagt
     1381 gccatgatcg agcacggcag caacctgttc gaagggcacg cgtgatcccc aatcggagag
     1441 ctgcgcgatc ggccagatgc gattcttctc gtccggcttg ccctggattt tcagcaactc
     1501 gcccagcttc tgaacctggc gcgaaatcca ggcgggatct tcagcatgcc cgaagcgggc
     1561 gtggtacggc atgatgctga agatatcgag gtacttcgcc tgggccttca ggtcgatggc
     1621 cagcttgtgc cgtattgcgc cctcgtattc gtcctgcgac caggggcaat ggaacgtgcc
     1681 tatcagggct ttaggccgca catcgtccag aatctcgcga tattcacgca cccagtccgt
     1741 gaagacgtcg cagcgaaagt tggtccaggc ttcgcgctct ttgcccagca acaccttcgc
     1801 cgccgcggct gtgtcggtcg ggagcgtgat ccccgatttc tcggagaact gattcaacgc
     1861 cgcgggtgaa aaatcggtgt cgggcagctc cggctctgcc cgctcccagc tcgcatgggc
     1921 gtggtgatag tcgagccaga tgccgtcgat ctcgaacgtc tgcagcacgc ggcggaactc
     1981 cgccatccgg tcgcgccgat agtcgcgatg gaaggcgctc acgccttgcc atccctgcgg
     2041 cggcggcgac ggcttgccgt ccgtgccgat cggcgcggca tccggatgct ctttcaagta
     2101 ctccgccgca tgcagcgaat tgaactcggc aaagaccttg agcccgagct tgcgatagcg
     2161 atcgatctcg gtggcatcca ggctgccgga gccgacccag atggcattga tcccgtaatt
     2221 cgccggcgtc tctccgcggt ccagtacttc gcggggatat ccgccgtaga ttccgcgaat
     2281 gctgatccac ggccgcgcag cctcgcgttc tggtcgctca tcggcgttgc aaaagtgcat
     2341 tggccgcgca ctgacgagag tcgcacccgc ggccagcgac tgcaatacca gccgccgcgt
     2401 gcagcgcgtg ttcttaaccc cgacggcgtt gtttccgggc atcgatctcg ttcctgaagg
     2461 caaaggccta gtttcttgcg gttcctcaca catcagcata tcttcttgag cgaggagctt
     2521 cgtcatgccg cgactgtttt cgatcgtctg ccttgccttc gcctgtctgg tcgctgcccg
     2581 cgaggtggca acggccgggg agattcaggc ccaagaaccg gagcgcagcg ggcagaccct
     2641 tcaccaaccc agcaacactt ggaccaaacg atcgccactc gccgacgtgc ccgtttcgcc
     2701 gcggctgggg tatgaaggcg catgcgtgtg ggactcgcgg caccagcttc tggttcgcta
     2761 tggcggccac aatcaagggg gcggcgggga gcaaggggcg gaagtatgga cgtgggatcc
     2821 catcaccggc aagtgggcat tgaaagagcc caacacgtct ccgcccggcg tgtgctgcaa
     2881 cgcgcagaac atctatgacc cgatcggcgg ccggtatatc cgctttccgc tgttcagcgg
     2941 cagtcacggc tggcagtggg cgcgcgagct gtaccttaac gactcctcgg tttggaccta
     3001 cgacgtgggc accaatcgct ggcgaaacat gcggccgcta cccgcgccac ggctggcgcc
     3061 gtatcgctgc gcgtcgtggg acagcgacga acaggtcgtc gtcgtctttg gcggcgaggg
     3121 gagccatgag ggaacgctca tttacgatcc agcccgcaac gagtggcgca agcccaaacc
     3181 agccgtggag ccagaaggcc gcagtggcgg caacatggca tatgatgccg cgcggaaggt
     3241 ccacgtgtta ttcggctcgc agttcaccga cgatccgcac acttggacgt atgacgtgcg
     3301 gaagaacgag tggcgcgaca tgcagccgcc gtcgcagccg ccaaccgaca aaaacgacgc
     3361 ggtgctgacc tacgatccga tccagcagcg agtgctggcg attgtgaaga ttacgaccgg
     3421 gaaggacgag gaggcgcgac atgaactgca aacttgggct tacgacgccg gggccaaccg
     3481 ctgggagcgc aagaatccga tgagcgagcc tcagccctcg agtaatcggg cccggcagct
     3541 gatcttcgcc ccggagctga atttggcaat tctggagaac tgcacgagcc ggccgcgcga
     3601 gcagcagatt tggacctatc ggctgggcga ctcgcccgct gcgccggcgg cgcggccgct
     3661 tgaagcgcgg tccgtcccgc cgttggtcga ggacgtcgtg gtctcggtcg tgtcgccgaa
     3721 caaggtcgaa gtcgcctgga ccgctccggc tggcgcggtc gccagcggtt atcacgtcga
     3781 gcgggcggtg gtcgaagtgt ggagcgacga tcagcttcgc cgcttgaagc agcggacgac
     3841 tccgttggca gagccggccg tcggcgcgat ccgccgcatc ggcccgtttc agcggttgac
     3901 caaagaaccg attcgtggca ctgcgtttac cgatacggag gtggaccttc gaaagccgca
     3961 gacgatcgac ggtgacccgg tttgggactc atcgttgcat gccgagcact tggacgaatc
     4021 ggggcgcggc taccggttgg gcgtgttcgc gtatcgcatc cgggcgttgg accgtgccgg
     4081 cgcggaattc ggcccctcgc cggcggtgct caccatcccc tcctcgccgc agcacctctt
     4141 cagccgggag gacggtacga cttgccagct caaatggtcg cccaattccg agcagggtat
     4201 tgctggctat cgggtgtacc gcatggatgg ccgttatgac aaggagcccg tgtctcgcct
     4261 gaccgaggcg acgctgacgg acaccaagta cgaagacgcc accgctggaa aatcgacacg
     4321 gcgatactac gtgatcgccg tcgatgccct gggtcaagaa ggattcactt cgtcccctgt
     4381 ttggtttcag cgcgaatggc gcgacttcta caggccgttt gtcgcagagt ggcaccaatg
     4441 attcggcgtg aagggcatgg gaaatcattc acgaggcgtg taccgaccat gcacgaacga
     4501 tcgggtcacg gaagattcgc cagtatcttc aatcattcgg tcgcgcgtga gggagaaagg
     4561 ccggccttgg gcatcgtaaa gcgtttcggg cgcgaagccg aggcagtgga ggatggtcgc
     4621 caggaagtcg gccggtcgca cgtacgcatc gatcggctcg gtggcgtgct taccggattt
     4681 cgcatgcacc gcgccgccgc gaattccgcc gccggcaagt gccagcgaca tgcagcggct
     4741 ccagtggtcg cggccggcct tgccgttgaa tttgagcgtg cggccgaatt cgccgaacac
     4801 acgataagtg tcgattcgcg caagccgcga tccgacaaat cctgcacgag agccgtaaga
     4861 cactggtcgg cgatcggcgg taagcgaagg tgagacacac cgcttgcaaa ctcgtccagc
     4921 gcaaactcgc agtggttgca tcgacctacg caggctgcgg gggcagcatg aagccgattc
     4981 cccgtcctac gctccttgcg gtcgcgacgc tgctcgccct cgccttcgtt cacatagtgc
     5041 gaatgccatt acgaattgat aggatgttgc cgacaacacg tatttaaacg tttttcttta
     5101 gctcccggtt acttccatga agctacttcg accagtttgc ccggagattc tcattggcat
     5161 cacgttgctc ttggcgtcgg gcctcgccgc tgccgagccg ttaaggccgg cgcaacagtc
     5221 ggccgtcgag gaaatcgacg ctcgtcagtc ggagctgaac gaagttaaca aggccatctg
     5281 ggactttgcg gaagtcggac tggaagagcg tcgctcgtcg gcgctcttgg ttgagaagct
     5341 caaggcggca ggcttcaagg ttcgtgtcgg cgtagccgac atgccgaccg ctttcgtcgc
     5401 cgaattcgga gcgggccacc cgatcatcgg catcttggcg gagtacgacg ccttgccagg
     5461 actgtcgcag aagatcgcgc cgcagcgcga tgcggtaacc gccgacgcac cgggccatgg
     5521 ttgtggtcat agtggattgg gaaccggcgc cctcggggcc gcactagccg tcaaggcggc
     5581 gatggagaag cactcgctgc ccgggaccgt gcggctgtac ggcacgcccg ccgaggaaac
     5641 gctgatcggc aaagtctata tgacgctcgc tggtgagttc gagcagctcg atgcttgcct
     5701 gcattggcac ccaagcgacc gcaatgaggt ctggaatggc gtatcgaagg cagccgtctc
     5761 ggccaagttc acgttcaccg gggtcacggc tcacgcctcc ggaagtcccg acaaaggacg
     5821 tagcgctctc gatgccgtcg agctgatgaa tgtcggggca aactacatgc gcgaacatct
     5881 gaaggaagac gcccgcgtgc actatgtcat caccaacggc ggcggcgctc cgaacgtcgc
     5941 tccagccaag gccacggtct ggtattacgt gcgtgccaat gagcatgccg acgtcgagcg
     6001 caattttatc tggctgcgag acattgccga cggcgccgca aaaatgaccc ggacgtcggt
     6061 gagcgttcag atcgatacgg attgccacga aatcatcccc aacgagccgc tagcagcgct
     6121 cctcctggta aacctggagc gggtcggtcc accgaagttt actccagacg aacacacttt
     6181 tgcgcggcga ctacaagagt cggtcacgca ggagtttggc acggtgttcg atctggccct
     6241 ggacgaagag gtgcaccgta cgcggaaact cggcgccgga gtcggctcga ccgacgtggg
     6301 cgacatcagt tggcacgtgc cgacgggcgg atttcgggcc gcctgtttcg cctctgactc
     6361 gcctgggcac agctggcaga atgtggccgc gattggttcg actatcggcg aaaagggaac
     6421 cggttacgcc gccaaagtgc tcgcttgcac gacgctcgac ctattggaag acccccaaac
     6481 tctcgccgcg gccaaactcg atttcgccga acgaatggaa ggccgcaagt acgtcactct
     6541 gatccctccc ggccagaagg ctcctaagtc gattcgctag tatgcactgc cgcgattcct
     6601 cacctcgctt tgcgcacacc tcggtccgcc tcgccttggc gcgatagcaa ggcgggcgtc
     6661 gtgaccgaag cgcatcctgg aatccctcat tgcgccgctg aaatcagcct ttttctggga
     6721 gtcctgtagc tggcgtccct gtgtcccaac cccagcatgc gtcgtcgagt ttcgtcagcg
     6781 agcagccgtt ttccgactgt gccgcgagac ctgcttcagc aaaggcctgc accgcaggcg
     6841 ccctgatgcc gactgggact cgaatcgatg atcgacccac gacgaagccg ccaacagccg
     6901 cggccatatt gaagccaatc tttcccagag gaagactacc ccatgcagac gccttccgcg
     6961 gctgatcagc cgcggccacg atgaagcaac tatcgttgct gtcaatcgtc acggcgggcg
     7021 gtagcctccc gatctccgtc acctacaggc aacttgacac gtcgattctc cgtaacgaca
     7081 ctcggcaacg tacccgctgt actgaaatgg ggagccaacc aatggccaag cgccgcaata
     7141 aagccagcaa gatccgtgca gtcctcgaag agctgggccc tgacgccaag gtcgcgcagg
     7201 tggtcgagac gctggccgcc aggcgggtcc gcgtccggcc gcagcaggtg tatgcgatca
     7261 aggcggcaaa taagaacggc cggtccaacg ctggcgacta caactccctg gtcaaggcga
     7321 agaagctggc tgatgcgatg ggcggagtgg aaaaggccaa ggcagcactg gatgtgctgg
     7381 ccaagctgat gtaggaaacg cctctttgac cgtatgcaaa gtgatcacca tgcccggtcg
     7441 agttacccgc cgcgaactgc ttcggacgac tgccgctgcg ggattggcca cacttgctcc
     7501 gtctcttccg gccgccgagg atcgggagcg ccgcacgttt gttgtcggac atcgtgggtt
     7561 gttgcattcg gcgccggaga acacgctggc cgcgtttcgc ggctgcctgg cgctgcgggt
     7621 cggcttcgag ttcgatgtgc ggcgcacgaa tgacggccag ctggtctgtc tgcacgatgc
     7681 gactctcgac cggacgacct ccggcactgg acggctggcc gatcggacgc tgaacgagct
     7741 gcgccggttg gacgcaggga gctggttcga tgactccttc cacggcgaac gcgttccgac
     7801 cgtgaacgaa gtcctggcgt tggtcgcaga acgggcgacg gattcgacgt tcatcgccgt
     7861 tgatctcaag gaaacgggac acgggatcga c
//
LOCUS       DASUGB010000830         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1341230, whole genome shotgun sequence.
ACCESSION   DASUGB010000830 DASUGB010000000
VERSION     DASUGB010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341230"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..>2154
                     /locus_tag="VFV87_15770"
     CDS             82..>2154
                     /locus_tag="VFV87_15770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5105278.1"
                     /translation="MPLADLTTRSLQSAIRNPQSAIVLAAALLLSPVACSLSPLAAQD
                     DPFAAGVRTTPWLKPEEEQKAFKLPPGFEIELVAAEPDIQKPLNMAFDERGRIWLTCT
                     VEYPYAAPLDRPGRDCIKVLEDTDGDGRYEKVQTFADGLNIPIGIYPYKGGCIAWSIP
                     NIWHFEDADGDGVCDKRTVLYGPFDYSRDVHGMCNAFRRGFDGWVYACHGFNNDSRVK
                     GKDGHEVHMQSGNTFRFQLDGSRIEHFTHGQVNPFGMCFDELFNIFTADCHSKPLTQL
                     IRGGYYPSFGKPHDGLGFVPSMMEHSHGSTAICGVVKYSGENFPKEYHGNFLSGNVMT
                     SRINQNKPEYHGSTIRAIEQPDFLSTTDPWFRPVDLQIGAGGALYILDFYNRIIGHYE
                     VPLPHPGRDRTSGRIWRVVYQGSGVRGQGSGEDRGQKTEDSAVGNAVPGVPGAANTPK
                     PGAYDLTKMSIDELIAQLDSPQLEHRLRVLNYLADVKDGGELGSVWTKLYAPASSRQL
                     AAIVWLLHRFERLDSARVAPAITSEDREARVHAVKAIVATISPTNEQRRLVRDRLRDA
                     DAFVRVAVADALASHPEATNLDELVRIAGGAPVEDPLLRQAARISLRDHLLSLSQVEG
                     GKFMGRWASRSTDSEFGELTLVATNVESPGASEIALGCIPIAEDRKLPILINYESLAR
                     TVTGNGIER"
BASE COUNT          440 a          651 c          676 g          387 t
ORIGIN      
        1 ggtagcggcg ggccgatcgc tccacgagcc gcgccagcac ggccgaagaa tcattcccac
       61 catcctgccg gacgaatcgt aatgcctctc gccgacctca cgactcgcag tttgcaatcc
      121 gcaatccgca atccgcaatc cgcaattgtc cttgcggccg ccttgctcct gtcccctgtc
      181 gcctgttccc tgtcccctct tgccgcccaa gacgatccct tcgccgcggg tgtccgcacg
      241 acgccgtggc tgaagccgga ggaggagcaa aaggcgttca agctgccgcc gggattcgag
      301 attgaactgg tcgcggcgga gcccgatatt cagaagccgc tgaatatggc gttcgacgag
      361 cgggggcgga tctggctgac gtgtacggtc gagtatccgt atgccgcgcc gctcgaccgg
      421 ccggggcggg attgcatcaa ggttttggag gataccgacg gcgacggccg ctacgaaaaa
      481 gtccagacat tcgccgacgg cctcaacatc ccgatcggca tttatccgta caaaggcggc
      541 tgcatcgcct ggagcattcc gaacatctgg catttcgagg atgcggacgg cgacggcgtg
      601 tgcgataagc ggaccgtcct ttacggcccg ttcgattaca gccgcgacgt gcatggcatg
      661 tgcaacgcct tccgccgcgg gttcgacggc tgggtttacg cctgccacgg cttcaacaac
      721 gacagccgcg tgaagggcaa ggacggccac gaagtccaca tgcaaagcgg caacacgttc
      781 cgcttccagc tcgacggcag ccgcatcgag cacttcacgc acgggcaggt gaacccgttc
      841 gggatgtgct tcgacgagct gttcaacatc ttcacggccg attgccacag caagccgctg
      901 acgcagctca tccgcggcgg ttactacccc agcttcggca agccgcacga cggcctgggc
      961 ttcgtcccct cgatgatgga gcactcgcac ggcagcacgg cgatctgcgg cgtggtgaag
     1021 tacagtggcg agaatttccc gaaggagtac cacggcaact tcctcagcgg caacgtgatg
     1081 accagccgca tcaaccaaaa caagcccgaa taccacggca gcacgatccg cgcgatcgag
     1141 cagcccgact tcctgagcac caccgacccc tggttccgcc cggtcgatct gcaaatcggc
     1201 gcaggcggcg cactctacat cctcgacttc tacaaccgga tcatcggcca ctacgaagtg
     1261 ccgctgccgc atcccgggcg agataggacg agcgggcgga tttggcgggt cgtgtaccag
     1321 gggtcaggag tcaggggtca ggggtcaggg gaggacagag gacagaagac agaggacagc
     1381 gctgtaggga acgccgtccc tggcgttccg ggcgctgcga acacgcccaa acccggcgcg
     1441 tatgacctca cgaaaatgtc gatcgacgaa ttgatcgccc agctcgactc gccgcagctg
     1501 gagcatcggc tgcgggtgct gaattatctg gcggatgtga aggatggcgg tgaattggga
     1561 agcgtttgga cgaagctcta cgcgccggcc agcagtcggc agctggcggc gattgtgtgg
     1621 ctgcttcatc gatttgaacg tctcgactct gcccgagtcg caccagcaat tacgagcgag
     1681 gaccgcgaag cccgcgtcca tgcggtgaag gcgattgtcg ctaccattag cccaaccaat
     1741 gagcagcgga ggcttgtaag ggatcgactt cgcgacgcgg acgcgttcgt gcgggttgcg
     1801 gtggctgatg cattggcttc tcatcccgag gcaacgaacc tcgatgaatt ggtaagaatc
     1861 gccggcggtg ctccggtaga agatccactg ctacgtcagg ccgcgaggat ttccctgcgt
     1921 gaccacctgc tttcgctaag ccaggtggag ggggggaagt tcatggggag gtgggccagc
     1981 cggtctacgg attccgaatt cggcgaattg actctggttg caacgaatgt cgaatcgccg
     2041 ggcgcgagtg aaatcgcgct agggtgtatt ccgattgctg aagatcgcaa gttgccgatc
     2101 ttgatcaact atgaatcctt ggcacgaacg gtcactggaa atgggattga aaga
//
LOCUS       DASUGB010000831         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_369519, whole genome shotgun sequence.
ACCESSION   DASUGB010000831 DASUGB010000000
VERSION     DASUGB010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369519"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFV87_15775"
     CDS             complement(<1..651)
                     /locus_tag="VFV87_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5105279.1"
                     /translation="MTAASGNSRERTALLPGDYVSPGLRVTALLGPGVYRCTPEEGGP
                     ELVVKLVELSSVSPSNVARVELEATLLEGVASPSIASPLFVLRDDQRLVVAYPWAAGT
                     TLEERLQQGPLDVQQALAVFGAVFSALRELHRHKLLHRAIRPGNIVLTPEGGPARAVL
                     VDHGPIAGAAGHDSAARADLLAAARYLSPEQAGSIDHDVNDASDLYSAGVTLFHCLA"
     gene            951..2039
                     /locus_tag="VFV87_15780"
     CDS             951..2039
                     /locus_tag="VFV87_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105280.1"
                     /translation="MIERIESFFTGSVHQFTSALANLAGRSSPADCHPADDFVHELTY
                     RINESLTACAELEAEIRSDGVLLKSVQRRYREAIWPWLGQSWFMRRALEKPRGYPGDY
                     ELLTAIYNGEVKSLGLGAALDRYFLKTTLAQAVVARMQAVRDFLEEELNSRQGNVTVL
                     NVASGACREYTLNFVPTNSRRIDVTCIDNDLLALEFVRTNVAPQLPQHIQLSFTRYNA
                     LRMVRAEQNVKRFGRPDVLYSVGLCDYIPDEYLIPMLRAWRESVNDGGVVYVAFKDAR
                     RYETPEYQWLVDWFFFQRTEEDCRRLFAEAGYGDSALTTTRDTTGVILNYVARMTAAV
                     DAHRRMDSAERLPTAPQLAPSTVRETAK"
BASE COUNT          404 a          672 c          694 g          377 t
ORIGIN      
        1 cgccaggcag tggaacagcg ttacacccgc ggagtacaga tccgaagcgt cgttgacgtc
       61 gtgatcgatc gagcctgcct gctccggcga aaggtaacgc gcggcggcga gcaggtcggc
      121 acgggctgcc gagtcgtgcc ctgcggcgcc ggcgatgggg ccatgatcga ccagaacggc
      181 ccgtgcaggc ccgccctcag gcgtcagcac gatgttgccg ggacggatgg cgcggtgcaa
      241 aagcttgtgg cggtgcagtt cgcggagagc cgaaaagacc gccccaaata cggccaatgc
      301 ctgctggaca tcgagcggtc cctgctgcag gcgctcttcg agcgtcgtgc cggctgccca
      361 cggatacgcg acgaccaggc gctggtcgtc gcgaagcaca aaaagtgggc tggcgatgct
      421 gggactggca acgccctcga gcagcgtggc ctccaactcg acccgcgcca cgttcgatgg
      481 cgaaacggag cttagctcga ccagcttaac gacgagctct gggccgcctt cctccggcgt
      541 gcatcgataa accccggggc ccaagagcgc agtgacgcgc agacccggcg atacgtagtc
      601 gcccggcaac agcgcggttc gctctcggga attgccgctg gcggcggtca tgcggccgag
      661 cctcgcaggg gcgcaggtag ctaggggcag gtggggccga gaaagccgcc tctacctcaa
      721 gaaaacttag ctctgcccta gcgccgctga gacttacggc gacggcaata agacgtggcg
      781 gttttacggg ctgtaccgcc cttccccatg cgggaacccg tatttttgcg agattgccca
      841 tttttcgttt ggggtccagg actgaccagg ggcaaaatgc gagcctccgc tgggagcgga
      901 ccattgacgc acttctcctg caccaacccc tcacccctca aggtgcgaag atgatcgaaa
      961 gaatcgaatc gttcttcacc ggtagtgttc atcaatttac gtcggcactg gcaaacctgg
     1021 ccgggcggag ctctccggcg gattgccatc ctgcggacga cttcgtccat gagctgacgt
     1081 atcgcatcaa tgaatcgctg accgcttgtg cggagctgga ggcggagatc cgcagcgacg
     1141 gagtattgct gaagagcgtt caacgccgct atcgtgaagc gatctggccc tggctggggc
     1201 aaagctggtt catgcggcgg gcgctggaaa agcctcgcgg ctatcccggc gattatgagc
     1261 tgctgacggc gatctacaac ggcgaggtga agtcgctggg gctcggcgct gccctcgacc
     1321 gctatttcct caaaacgacg ctggcccagg cggtcgtcgc ccggatgcag gccgtgcggg
     1381 atttcctgga ggaagagctc aatagccgac aggggaacgt gacggtgctg aacgtggcca
     1441 gcggtgcttg ccgcgagtac acgctcaatt tcgttcccac gaacagccgg cggatcgacg
     1501 tgacctgcat cgataacgac ctcctcgccc tggagttcgt gcgcacgaac gtcgcgccgc
     1561 agcttccgca gcacatccag ctttcgttca cgcgctacaa cgcgctgcgg atggtcaggg
     1621 ccgagcagaa tgtgaagcgc ttcgggcggc cggatgtgct ctacagcgtc ggtctgtgcg
     1681 attacattcc ggacgaatat ctcattccga tgctccgtgc gtggcgcgag agcgtcaatg
     1741 acggcggcgt ggtgtatgtc gccttcaaag acgcccgccg ctacgaaacg cccgagtacc
     1801 agtggctcgt cgattggttc ttcttccagc gcaccgaaga agactgccgc cggctgtttg
     1861 cggaggcagg ctatggcgat tcggcgttga caacgacccg cgacacgacc ggcgtcattc
     1921 tcaactacgt cgcccgcatg actgccgccg tggatgcgca ccggcgaatg gacagtgccg
     1981 aaagactccc caccgcaccg cagctcgcgc cgtccaccgt tcgcgagact gccaaatagt
     2041 accgaagcgt cagcgaggga gtccctgtaa cccgacgcgt cggcgagcgg atccgtcgct
     2101 gacgcttcgg gttacattcg gctctggccg gctcctattc gggcagg
//
LOCUS       DASUGB010000832         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1202989, whole genome shotgun sequence.
ACCESSION   DASUGB010000832 DASUGB010000000
VERSION     DASUGB010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202989"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..963
                     /locus_tag="VFV87_15785"
     CDS             136..963
                     /locus_tag="VFV87_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105281.1"
                     /translation="MILPVRWWLLTFLLLVPAVDAAAESLDARLLADARDGRLDEFDF
                     LSACLIAGGVSDPAELAEATSEAADVRARLDLASLRELPPLPRARELHDRLHARILQG
                     TYRQDASDLRLALRGEAFNCLSALALYCEFGREAGLDLEIWSQPGHVYLVERASGARN
                     DPASHQSAARAESAARQLTPVQLVGKFYYNCGVLALKAGRYEEGVSLVRRSLQLDPLD
                     GEAQKNLLAGLNNWAVDLCRHQRYAAARELIEAGLAIDAGFEPLVANARYVTSKSGQ"
     gene            1067..1852
                     /locus_tag="VFV87_15790"
     CDS             1067..1852
                     /locus_tag="VFV87_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX5105282.1"
                     /translation="MPARPLRVTIWNEYIHEITSTAVGQVYPGGMHVVWSEAIQRLLG
                     DSVQIRVALLEQPEHGLPYDVLAETDVLTWWGHAGHDRVDDEIVERVQRRVWEGMGLV
                     CLHSAHNSKIFKRLMGTTCMLRWREAAEKERVWICDPGHPIVEGLADEFFEIEHSEMY
                     GEHFDIPQPDELFAISWFEGGEVFRSGCTWRRGKGKVVYFSPGHETFPIYKNASVQQI
                     LANAVRWAAPSGGLYIGQGRNVARPLSPIAAEHVVDESLHKPK"
     gene            1924..2301
                     /locus_tag="VFV87_15795"
     CDS             1924..2301
                     /locus_tag="VFV87_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5105283.1"
                     /translation="MAKRILRHTDLQVYQRAHAAAQTIFEHSKRFPKEERYSLTDQIR
                     RCSRSVCGNLAEAWRKRRYPNAFVCKLSDSEAEAAETQVWLQFAVDCKYIDRDAAAEL
                     YREYEAIIGMLVAMINRPEDWQL"
     gene            complement(2350..>3816)
                     /locus_tag="VFV87_15800"
     CDS             complement(2350..>3816)
                     /locus_tag="VFV87_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5105284.1"
                     /translation="GTIINRVTSDVQGVRAFIDGVLIQFVLLTVSLLCYLSYMLSLHV
                     GLTLACLATTPIMWLVTVIFSRLVRPMYDRNRELVDRIILRLAESIQGVQVIKGFGRE
                     REEIAQFAADNRAVRDQQRGIFWRVSIFTPMIGYMTQINLVILLAYGGWLVTQNQLAL
                     GSGLVVFAGLLQQFSSQVANLTNIANSVQQSLSGARRVFEILDAPIAIKNPKNPIRLH
                     SRDQWSRNTGASRENGEPRPMVAALSGRIDFEHVWFEYTPGNPVLQDVNLVIEPGEKV
                     AILGATGAGKSTLLALLCRFYDPTHGRILLDGHDLRDLDLDDLRRNIGLVFQESFLFS
                     NTVAANIAFGHPEATVAQVEKAAKIAAAHDFIAELPDGYDAVLGEGGLDLSGGQRQRL
                     AIARAVLLDPAILLLDDPAAAIDPHTEHEILAAMEQAMQGRTTLVIAHRLSTLRQCDR
                     VIVLDSGQIAQRGTHAELMEAEGHYRHAAQSQLAGARA"
BASE COUNT          681 a         1200 c         1258 g          677 t
ORIGIN      
        1 gccgatcgcc gctacagccg gcaaatttgg ccccgtttga cgcacgcgag ggcaattccg
       61 cgctgcattt cagccgcatg ctgcccaatc gcatcgtacg attgtgcggg tttcggtctc
      121 gctcaagtgc ccacgatgat cttgcccgtc cggtggtggc tgctgacgtt cttgttgctc
      181 gtccccgcag tcgatgcggc tgccgagtcg ctcgatgcac gcctgctggc tgacgcccgc
      241 gatggccggc tggacgagtt cgatttcctg tcggcctgcc tgatcgcagg cggcgtgagt
      301 gatccggccg agctggcgga ggcgacctct gaagccgcgg atgtgcgggc gcggctggat
      361 ctcgcttcgc tgcgcgaact gccgccgctg ccgagggccc gcgagctgca cgaccgcctg
      421 cacgcacgaa ttctgcaagg aacgtatcgc caggacgcca gcgatctgcg gctggcgctg
      481 cgcggcgaag ccttcaactg cctctcagcc ctggcgctct attgcgaatt cggccgcgaa
      541 gcgggccttg acctggaaat ctggtcgcag ccggggcatg tctatctggt cgaacgggcg
      601 agtggcgcgc ggaatgatcc ggcgtcccac cagtcggccg ctcgtgcaga atctgccgcc
      661 cggcagctca cgcccgtgca gctcgtcggc aagttttact acaattgcgg agtgctcgcg
      721 ctaaaggcgg gtcgctatga ggaaggtgtg tcgctcgtcc gcaggagcct acagctcgat
      781 cccttggatg gcgaagcaca gaagaacctg ctggccggcc tcaacaactg ggccgtcgat
      841 ctctgccggc accagcgcta tgccgcggct cgggaattga tcgaggccgg gctggcaatc
      901 gacgcgggct tcgaaccgct ggtggccaat gcgcggtatg tgacgagcaa atcgggccag
      961 tgaggccaag tgcaatcgtg ctgacgggcc gttagaatcg cggcgtcttc acgccccgct
     1021 ccgggaccag gtttggtccc ggctaagagc tctcacgagg tgactcatgc ccgccaggcc
     1081 gctgcgcgtc acgatttgga acgagtacat ccacgagatc accagcaccg ccgttgggca
     1141 ggtttatcca ggcggtatgc acgtcgtgtg gtccgaagca atccagcggc tcttgggcga
     1201 tagcgtgcag attcgcgtcg cccttctgga gcagccggag cacggcctgc cgtacgacgt
     1261 gctggccgag accgacgtgc tgacgtggtg gggtcacgcg gggcacgacc gcgtggatga
     1321 cgagatcgtc gagcgcgtgc agcggcgcgt ctgggaaggc atgggcctgg tgtgcctgca
     1381 ctcggcccac aattccaaga tcttcaagcg gctgatgggg acgacctgca tgctccgctg
     1441 gcgcgaggcg gccgagaagg agcgggtttg gatctgcgac ccggggcatc cgattgtcga
     1501 aggcctggca gatgaattct tcgagatcga gcacagcgag atgtacggcg agcacttcga
     1561 catcccgcag ccggacgaac tgttcgccat cagttggttc gagggaggcg aagtcttccg
     1621 cagcggctgc acgtggcggc gcggcaaagg aaaggtcgtc tacttcagcc ccggccacga
     1681 gaccttcccg atctacaaga acgccagcgt ccagcaaatc ctcgccaacg ccgtccgctg
     1741 ggcagcgcca tcgggcggcc tctacatcgg ccaaggccgc aacgtcgcca ggccgctcag
     1801 tccgatcgcg gccgagcatg tggtggacga gagcttacac aagccgaagt gaatctagag
     1861 ggggagaggg ggcgagtggg agagggggag acgagcgaag gcatctcgaa actgaggcat
     1921 cccgtggcga aacgtattct tagacacaca gatctccagg tgtatcaacg tgctcatgct
     1981 gccgcgcaga cgatcttcga acactcgaag aggtttccga aagaggaacg ctactcgctt
     2041 acggatcaaa tccgtcgatg ttcgcgaagc gtctgtggca atcttgccga ggcttggagg
     2101 aagcgccgct atccaaacgc gtttgtgtgc aagctcagcg acagtgaagc agaagcggcg
     2161 gaaacgcagg tctggctaca attcgcggtg gattgcaaat acattgaccg tgacgccgca
     2221 gcagaactgt atcgcgagta tgaagcaatc atcggcatgc tcgtcgcgat gatcaaccgg
     2281 cccgaagact ggcaactttg acgactgccg gtctccccct ctcccactcg ccccctctcc
     2341 ccctcttgcc tacgcccgcg cccccgccaa ctgcgactgg gccgcgtggc ggtagtggcc
     2401 ttcggcttcc atcaactcgg cgtgcgtgcc gcgctgggcg atttggccgc tgtcgaggac
     2461 gatcacgcgg tcgcactggc ggagcgtgct cagccggtgg gcgatgacca gcgtggtgcg
     2521 cccctgcatc gcctgctcca tcgcggcgag gatttcgtgc tcggtgtgcg ggtcgatcgc
     2581 cgcggctgga tcgtccagga gcaggatcgc cgggtccagc agcacggccc gcgcgatcgc
     2641 cagccgctgg cgctggccgc cggagaggtc cagcccccct tcgccgagga ccgcgtcgta
     2701 gccgtcgggc agttcggcga tgaagtcatg agcggcagcg atcttcgcgg ctttctcgac
     2761 ctgggccacg gtcgcctccg gatgcccgaa ggcgatgttc gcggcgaccg tgttgctgaa
     2821 caggaacgat tcctgaaaca ccaggccgat gttccgccgc aggtcgtcca ggtccaggtc
     2881 gcgcaggtcg tggccgtcga gcaaaatgcg gccgtgcgtc ggatcgtaga accggcaaag
     2941 gagggccagc agcgtcgatt tgcccgcccc ggtcgcgccg agaatcgcca ccttctcgcc
     3001 cggctcgatg acgaggttca catcctgcag caccggattg cctggcgtgt attcgaacca
     3061 gacgtgctcg aagtcaatcc ttccactcaa agccgcgacc atcggtcgcg gttccccgtt
     3121 ttcacgcgag gcaccggtgt tccgcgacca ctggtcgcgg ctgtggaggc ggatgggatt
     3181 cttgggattc ttaatcgcaa tcggcgcgtc aaggatctca aatacccgcc gggccccgct
     3241 caggctctgc tgcacgctgt tggcgatgtt cgtcaggttc gcgacctggc tgctgaattg
     3301 ctggagcaag cccgcgaaga ccaccaggcc gctgccgagg gcaagctggt tctgcgtcac
     3361 cagccaaccg ccataggcca gcaagatcac caggttgatc tgcgtcatgt agccgatcat
     3421 cggcgtgaag atgctgacgc gccagaagat gccgcgctgc tggtcgcgga cggcccggtt
     3481 gtcggcggcg aactgggcga tctcttcccg ctcgcggcca aagcccttga tcacctgcac
     3541 gccctggatc gactccgcca gccgcaggat gatgcgatcg accagctcgc gattgcggtc
     3601 gtacatcggg cggacgaggc gcgagaagat gaccgtgacg agccacatga tgggcgtcgt
     3661 cgccaggcag gcgagcgtca aaccgacgtg caggctgagc atgtagctga ggtagcagag
     3721 gagcgagacc gtcagcagca cgaactggat cagcacgccg tcgatgaagg cccgcacgcc
     3781 ttgcacgtcg ctcgtcacgc ggttgatgat cgtgcc
//
LOCUS       DASUGB010000833         4922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_509403, whole genome shotgun sequence.
ACCESSION   DASUGB010000833 DASUGB010000000
VERSION     DASUGB010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509403"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..497)
                     /locus_tag="VFV87_15805"
     CDS             complement(168..497)
                     /locus_tag="VFV87_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX5105285.1"
                     /translation="MPYSESLADRIRQTFGRRRGLTEKKMFGGVGFLLNGNMCVGVWK
                     TSLIVRLDPVDADESLSQPHVRPFDITGRPMKGWLIVEADGLETDDQLASWIQRSIEF
                     VAGMPRK"
     gene            complement(516..842)
                     /locus_tag="VFV87_15810"
     CDS             complement(516..842)
                     /locus_tag="VFV87_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105286.1"
                     /translation="MSLLSSLGAIFTRGGRDEALLNQAMDHAKCKRPEKALEIYDSLL
                     KSPALSGSLRARVLYNRGLTHSALRNEEQALTDIQEVLKLPDVPDNVQAAAKERLVRL
                     RKRAGN"
     gene            complement(921..1613)
                     /locus_tag="VFV87_15815"
     CDS             complement(921..1613)
                     /locus_tag="VFV87_15815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5105287.1"
                     /translation="MAKTILGIGAHYDDCIFGISGTLLGAVRKNHRVVILSLIGDYSN
                     WKPAIGREKEIVQGTIEISKQYGVEMRFLNFAGTSYEVTEESKRAVAEAVAEIEPDVA
                     FILWPHDSHSDHEAASRLSKIALSHGDRLLPRGRRFKPPQQTYQYDNGPRHTIGFEPD
                     TFVDIANDWRQASEWLGRVMALVRGQPFDPSNPDGALQAKEAIARYRGQTCGVRYAEA
                     FRAGQAYPREIL"
     gene            complement(1937..2296)
                     /locus_tag="VFV87_15820"
     CDS             complement(1937..2296)
                     /locus_tag="VFV87_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105288.1"
                     /translation="MTPMILPEPGHVDEPKQAGDYAAVLSQVAAKGQPLIVRRNGADL
                     AAVISLEHLELMREVLATQEAERLAGQLDFDRLVRESPPAAAWLEGEEPKPFGSGWSD
                     RNGRRASTGEHRVGGNR"
     gene            complement(2405..3739)
                     /locus_tag="VFV87_15825"
     CDS             complement(2405..3739)
                     /locus_tag="VFV87_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105289.1"
                     /translation="MPPPAAKPSNVRWAVFSLACGTSWLLYLHRYVFALIKPELQAEW
                     GLSKVQLGFLDSAFSLFATLFQVPLGAAADVLGVRLVLTGLILLWCLGLWMHGRAASP
                     DQLWYARITMGIGQSAVYANLSRMAQLWFPPRIRTTLQGLAGISAGRLGGLSANLLFA
                     SLLLGVLGLDWRTAIYIFAIGGIAFAGVFFLVFRNSPREHPLANAAEADLMQGPSAAT
                     NPQQPRMTISQMLGSLRPRALANLLALNVQTVLSTIADNIYSNWIPLFLAEVHRLEYK
                     TMGIYSALPLLGGAIAGLTGGVLNDWLIARTGNRRWSRSGIALCGKGTAAVVLLAALL
                     AYDSPYVFCWLLFVVKLFGDWSLATSWGVVTDIGGRATASVFAFNNTIAGFALILASP
                     MLGYVAQQFGWRTVFVLVAAMYALCALSWLLVDCTIPVIGPTKESEEASSVM"
     gene            complement(3822..4904)
                     /locus_tag="VFV87_15830"
     CDS             complement(3822..4904)
                     /locus_tag="VFV87_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5105290.1"
                     /translation="MRVTHVEVVVVEVPQTAPLAPYRSHLRSSRATQSAIVRVETDEG
                     LIGWGEHNINFLPNISARRMTGDARAWLVGRDPQNINRFHQDCPLETRLKSGVELALW
                     DICGKAVGLPVVVLLGGVLRPRVEVAACMGIQTYERAGEMARWYVEQGFGSLKTKAGA
                     NANEDFEMVRGVRDAVGDKLKLRIDPNRAYTPQQALALAKRLEPFHLEYFEQPIPAEP
                     LTDATWLRNQTSVPIALNESVTNPASVMEIINKGAAAFILPDTHTAGGILPCVTIGRI
                     GEAAGIPCIMHCGHDLGPKTAAMLHVAAACPAYSLANDTTYYGLEDDVITERLSIESG
                     TMAVPLKPGLGVECDPERLARYRIDC"
BASE COUNT          932 a         1563 c         1520 g          907 t
ORIGIN      
        1 ccggctacgt ccgcggcaag atcctctacg gtcagttccg ctcgctcaag ggccacaacg
       61 gcgaattcag gctgaccgaa acggagaagt aggcagcgcc cttttcctgg ccgacgctaa
      121 cttttcctgg tcgacgctaa gcccggtccg ctgctgccga gtatcgatca ctttcttggc
      181 attccagcca cgaactcaat cgaccgctga atccaggaag ctagttggtc atcggtctcc
      241 agaccatcgg cttcgacgat cagccagccc ttcatggggc ggcccgtgat atcgaacggc
      301 cggacgtgcg gctgactgag cgattcatct gcgtcgacgg gatcgaggcg aacgatgagc
      361 gaggttttcc atactccgac gcacatgttc ccgttcagca agaagccgac gccgccgaac
      421 attttcttct cagtgaggcc gcgccggcgg ccgaaggtct gccggatgcg atcggcgagc
      481 gattcgctgt aaggcatgcg gtttggttcg cgccgttagt tccccgcccg tttccgcagc
      541 cggacaagcc gctccttggc ggccgcctgg acattgtcgg gaacgtcggg aagcttgagc
      601 acttcttgaa tgtcggtcag ggcctgttcc tcgtttcgca gcgccgaatg cgtcagccct
      661 cggttgtaca gcacacgggc gcgcaagctg ccgctgaggg cgggcgattt cagcagcgaa
      721 tcgtagattt ccagagcctt ctcgggccgc ttgcacttgg cgtggtccat tgcctggttc
      781 agcaaggcct cgtcgcgccc gccgcgcgtg aagatcgcgc ccaaactgct caagagactc
      841 atggttttgc ccccaaaatc cacgcctagc gtagcggagc gggcgacggg atgcaaactc
      901 accttcccgg ctaacggatt ttaaagaatc tcgcgcgggt aagcctggcc cgcgcggaac
      961 gcctctgcat agcggacgcc gcaggtctgg ccccgatagc gggcgatcgc ttccttggcc
     1021 tgcagcgctc cgtcgggatt ggacggatcg aacggctggc cgcggaccag ggccatgacg
     1081 cggccgagcc actcgcttgc ctgccgccaa tcgttggcga tatcgacgaa cgtgtcgggc
     1141 tcaaaaccga tcgtgtgccg ggggccgttg tcatactggt acgtctgctg cggcggcttg
     1201 aaccgccgcc cgcgcggcaa caggcgatcg ccgtgcgaaa gggcgatctt gctcagccgc
     1261 gaagcggctt cgtggtccga atggctgtcg tgcggccaga ggatgaaggc gacatcgggc
     1321 tcgatttcgg ccaccgcctc ggcgacggcc cgcttgctct cttcggtcac ttcatagctc
     1381 gtcccggcaa agttcagaaa ccgcatttcc accccgtatt gcttggagat ctcaatcgtt
     1441 ccctgcacga tctccttctc ccgcccgatc gccggcttcc agttgctgta atcgccgatc
     1501 agcgacaaga tcaccacgcg gtggttcttg cgcacggcgc ccaggagcgt gcccgagatg
     1561 ccgaagatac agtcgtcgta gtgggcgccg atgccgagga tggttttggc catgtcaatt
     1621 ctcgggttga ttgggtttca agctggaagc gattcgacga agaatctcga ccaatacagc
     1681 accgggcgtg ataccggcca cttcgcgcac gtttccggat tggacgcgag acacccacgt
     1741 gcaaacggct gctgatctgc gattcagtcc tgcccgcgga tggcctttgg cgtgccaggg
     1801 cagcggaacg tggtcatctg gtagtgagtc aggcaagcgg ctggtgatcg caacgacgtc
     1861 gagtggcgca gactgcgaaa ttcggtcagt aggagtgatc acgatggcgg ggcacagctt
     1921 gctgactcca ttcgcgtcag cgatttccac ccacacgatg ctccccagtt gaagcgcgac
     1981 ggccatttct gtcactccat ccagatccga aaggcttcgg ctcttctcct tccagccatg
     2041 ctgctgctgg cggagactcc cgcactagtc gatcgaaatc gagttgccct gccagccttt
     2101 cggcctcttg cgtggcgagc acttcccgca tcaactccaa gtgctccagg gagatgactg
     2161 ccgccaggtc ggctccattt cggcgcacaa tcagtggctg gccttttgct gccacctggc
     2221 tcagcactgc ggcataatcc ccggcctgct taggttcatc aacatgccct ggctcgggca
     2281 agatcatggg tgtcatcgct gcactagttg gaactgagta gggacgctac aagctatggc
     2341 gttcactggg attataagga ccggagcacc gacaaccaag aaattctccg tccagttcga
     2401 gtcactacat cacactggac gcctcctcgg attccttggt cggcccgatg accggaatcg
     2461 tacaatcaac cagcagccaa gacaaagcgc acagcgcgta catggcggcg accagaacga
     2521 agaccgtgcg ccagccgaat tgctgggcga catagccgag catcggcgag gcgaggatca
     2581 gggcgaagcc ggcgatcgtg ttgttgaagg cgaagacgct ggcggtggcc cggccgccga
     2641 tgtcggtcac cacgccccag cttgtggcca ggctccaatc cccgaaaagc ttgacgacga
     2701 acagcagcca gcagaagacg tacgggctgt cgtatgccag gagcgcggcc agcaacacga
     2761 cggcagccgt gcccttgccg cacagcgcga tgccgctgcg cgaccagcgg cggttgccgg
     2821 tccgcgcgat cagccagtcg ttgagcacgc cgcctgtgag gcccgcgatt gctccgccca
     2881 ggagcggcag ggcggaatag atacccatgg tcttgtactc gagtcggtgg acttccgcca
     2941 gaaacagcgg aatccaattc gaatagatgt tgtcggcaat cgtgctcagc acggtctgca
     3001 cgttgagggc caacaaattc gcgagcgcac gtgggcgcag cgagccgagc atctggctga
     3061 tcgtcattcg cggctgctgc gggtttgttg ctgctgatgg gccttgcatc agatcggctt
     3121 cggctgcatt ggcgagcgga tgctcgcgcg gggagttgcg gaagaccaaa aagaacaccc
     3181 ccgcgaaagc aatgccgccg atcgcaaaga tgtagattgc cgtccgccaa tcgagcccca
     3241 agacgcccaa gaggagggat gcaaagagca gattggccga cagcccgccc agccgtcccg
     3301 cgctaattcc cgccagccct tgcagcgtgg tgcggatgcg cggcgggaac cacaactggg
     3361 ccatgcggct gaggttggca tagacggctg actggccgat acccatcgtg attcgagcgt
     3421 accagagctg atcgggcgac gcggcgcggc cgtgcatcca taggccgagg caccagagca
     3481 ggatcaagcc ggtcagcacc agccgcacgc ccagcacatc ggcggctgct cccagcggga
     3541 cctgaaagag cgtggcgaag agcgagaacg cgctgtcgag aaaacccaac tgaaccttgc
     3601 ttaagcccca ctctgcctgc agctccggct tgatgagcgc gaagacatag cggtgaagat
     3661 agagcagcca cgacgtgccg cacgccaggc tgaaaaccgc ccagcggacg ttgctcggct
     3721 tggcggcggg cgggggcata ccttaacccg aagcgtaagc gagggcgtgc cccagcgaaa
     3781 cgtgaaaatc ggaactccct cgcttacgct tcgggttggt gtcagcagtc gatgcgatac
     3841 cgggccagcc gctcgggatc gcactcgacc cccaaccccg gcttcagcgg cacggccatc
     3901 gtacccgact cgatcgagag ccgctcggtg atgacgtcgt cctccaggcc gtaatacgtc
     3961 gtgtcgttgg ctagcgaata ggcagggcaa gcggctgcga cgtggagcat cgcggcggtc
     4021 ttcggcccca agtcatggcc gcagtgcatg atgcagggaa tgcccgccgc ttcaccgatg
     4081 cggccgatcg tcacgcaggg caaaatgccg ccggcggtgt gcgtgtcggg caggatgaat
     4141 gcggcagcgc ccttgttgat aatttccatc acgctggccg gattggtcac gctttcgttg
     4201 agcgcgatcg gcacgctcgt ttggttccgc aaccaggtcg catcggtcag cggctccgca
     4261 ggaatcggct gctcgaagta ctccaggtga aagggctcca ggcgcttggc gagagcgagt
     4321 gcctgctgcg gcgtataggc ccgattgggg tcgatccgca gcttgagctt gtcgccgacc
     4381 gcgtcgcgca cgccgcggac catttcaaag tcttcatttg cgttcgctcc cgctttcgtt
     4441 ttcaggctgc cgaagccttg ctcgacgtac caacgggcca tttcgccggc ccgctcgtaa
     4501 gtctgaatcc ccatgcacgc tgccacttcc acgcgcggcc ttaacacgcc gcccaacagc
     4561 accaccaccg gcagcccgac cgctttgccg caaatgtccc acagggccag ttcgacgccc
     4621 gacttcagcc gcgtttccag cgggcaatcc tgatggaagc gattgatatt ttgcggatcg
     4681 cggcccacga gccaggcccg cgcatcgccg gtcatgcggc gggcactgat attcggcagg
     4741 aagttgatat tgtgctcgcc ccagccaatc agcccctcgt ccgtctccac gcgaacgatc
     4801 gcgctctgcg tcgccctgct gctgcgaaga tgcgagcgat acggcgccag cggcgcggtc
     4861 tggggcactt cgacgacgac cacttcgaca tgggtgacgc gcatttaccg gtcgccaggg
     4921 ag
//
LOCUS       DASUGB010000834         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1621347, whole genome shotgun sequence.
ACCESSION   DASUGB010000834 DASUGB010000000
VERSION     DASUGB010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621347"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(492..770)
                     /locus_tag="VFV87_15835"
     CDS             complement(492..770)
                     /locus_tag="VFV87_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105291.1"
                     /translation="MFRYKLRTLLMVLTLIVVGATLFGTAMASSQRLALGDALLERLD
                     RQTATSVLDYLDDTRASRSLGFAIVAGLSAAAGIAVDRLVGHLMASKT"
     gene            complement(1232..1486)
                     /locus_tag="VFV87_15840"
     CDS             complement(1232..1486)
                     /locus_tag="VFV87_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105292.1"
                     /translation="MGFQLWGLFLAFVLIVNAGAVGLMLSAFGIVLWRGGWDAAMQAA
                     EAGRWPIARKLMVSGASLGLVFCFLYLLLAAWGKLLLDGP"
     gene            complement(1536..2828)
                     /locus_tag="VFV87_15845"
     CDS             complement(1536..2828)
                     /locus_tag="VFV87_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5105293.1"
                     /translation="MNPARIAFTAILLVASQALAIAADWPQWRGPEGTGVSPEKDLPI
                     VWHESRSIAWKCPLPEWGTSTPAIWGNAIFVTSHTADNQLVLMRIDKKTGQIVWTQTI
                     GSGEATREAPKRSTQKFHQLHNLASPSPVTDGKTVVAHFGNGDLAAYDFDGQRLWRHN
                     LQEEYGAYSIWWGHANSPVLFGDLVISICMQDSLADLQDKPVESYLLAHDLASGEVRW
                     KIPRMTRADAEECDAYTTPLLLTVDERPQLVVMGGNQLDAYDPATGKQLWLLPGLKGG
                     RTVTGPTAAGGLIFATRGMKGDLLAVKTGLAGELNYRDIAWKYGEGTPDTCSPVVWND
                     LLFTVTDDGIARCFDAASGNLQWKERLKGKYKASPIAAEGRIFFLNTEGLCTIVSASQ
                     RFDKLVENQLEDETIASPATSDGKLYLRGRKALYCISR"
     gene            3051..3446
                     /locus_tag="VFV87_15850"
     CDS             3051..3446
                     /locus_tag="VFV87_15850"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5105294.1"
                     /translation="MTVTQEMIDALFEPEMVETLCGQPKQGPAVLPGAERGSARVPFR
                     GRAVAIVFPPPSSPQAEPVESEVVTTDISRGGLSLLHRRELHRGQQVLLQLTRGTCTV
                     EVCWCCRVWPGLYIAGCRFADMTFADAAS"
     gene            complement(3461..>4010)
                     /locus_tag="VFV87_15855"
     CDS             complement(3461..>4010)
                     /locus_tag="VFV87_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105295.1"
                     /translation="LSDAGLAHVANLDNLERLNLNFTRVSDAGLSHLQGLTSLTWLGL
                     EATAVTDDGLQHLQGMKELQYLDLNGTKVTGAGLAHLQGLQLKTLYLDLSPTNDAGVE
                     ILQDWSSLNWLGLQGTQVTDSGLKHLEGLAGLTRLNLKQTKVTAVGVKKLADALPQCR
                     IEWDGGVIEPQAADPAMPQPAP"
BASE COUNT          739 a         1280 c         1251 g          740 t
ORIGIN      
        1 ccatcgtgga ccccgcgggc gattccaccg ccccacggga tcggtttgcc atgtgtgacc
       61 tccttggggt ttcactccat acggcaaacc tccaactgag gtttagctag caaaaacacg
      121 ttttgaaata cgctctagcc agcttgccag aaaagtagaa tgtccccttt atcgccgccc
      181 cagttcccgc accgcaggca agcccagcac cagtgacgag ggccaagagg tgcttcaggc
      241 cgaagtggac tgggcggtcg gtcatcccca aattctaacg caatgtgcca acgcccgcac
      301 aatcagcacc gtgcggagga ggtagtggag cacgtgccga gttggaggac cagcactccc
      361 caaccccacg ctggcactcc tggccgctac atgtttgcaa ttctaacggc cggctcacga
      421 atatgcccag ccgatccgcc ggactgccat cacatggtca cgacctgata cctgttgctc
      481 gtcctgcagc gctaagtctt gctcgccatg aggtgcccga caagccggtc aacggcaatt
      541 cctgcagccg ccgacagacc ggcaacaatc gcaaatccga gtgagcgcga cgcccttgta
      601 tcgtcaagat aatcgagcac ggaggtggcg gtttgacgat ccaatcgctc aagcagggcg
      661 tcccccagag ccaaacgctg gctgcttgcc attgccgttc caaacagggt tgcgccgaca
      721 acgattaggg tcagcaccat cagcagcgtg cggagcttgt agcggaacat gggagtctca
      781 ccaatgaagc ggggttgtgg ccacgtaatc ccatgcctct tgtccccaga caacgaagta
      841 gattactgcg gtcagcaagc cgaccgcaat gaaagcggag tccatcagca gcgtgcgcag
      901 gcgggatcgc atttccgcga ttctatcagc cggcgcaacc agattcatag gagcgatggt
      961 gggcgtggag acttgctcgg cccctggttg ctcgctgccg tcgaaaggag caggtacatc
     1021 gcgaggacaa gtgcagccag acaacatacc aatgaggccg caaggctcga accacacacc
     1081 accgtcatca gcagtgttcg ctcccccggt aacgtaacga cggccactcc cgtaaaggcc
     1141 agcgccgcga ccgtaaagcc ggccgcagtt agcaccttcg tgtttatttt catcccgtcc
     1201 attgtaaacg ctgacgctcg catcgaattc gttacgggcc gtccaagagc agcttgcccc
     1261 atgccgcaag taataaatac aggaaacaga agaccagacc cagggaagcg cccgaaacca
     1321 taagcttgcg agctatcggc catcgaccgg cttcggcggc ttgcatcgcc gcgtcccaac
     1381 ctcctcgcca gagcacgatt ccaaatgcgg acaacatcag gccgacggca ccggcattca
     1441 cgataaggac aaacgccaga aacaggcccc agagttgaaa tcccattgta ggcctccatg
     1501 agtactttcg gcgtatgcct gatcggttcc acgctctacc ggctgatgca atacagggcc
     1561 ttgcggccgc ggaggtagag tttgccgtca ctggtggcgg gggaggcgat ggtttcgtct
     1621 tcgagctggt tctcgacgag cttgtcgaac cgctgggagg cggagacgat cgtgcagagc
     1681 ccctcggtgt tgaggaagaa gatgcgcccc tcggcggcga tcggcgaggc tttgtacttg
     1741 cccttcagcc gctctttcca ctgcaggttg ccgctggcgg catcgaagca ccgggcgatg
     1801 ccgtcgtcgg tgaccgtgaa gagcaggtcg ttccagacaa ccgggctgca ggtgtcgggc
     1861 gtcccctcgc cgtatttcca ggcgatgtca cgatagttca gctcgcccgc caggccggtc
     1921 ttcaccgcca gcaggtcccc cttcattccg cgcgtggcga agatcaggcc gcccgcggct
     1981 gtggggccgg tgacggtccg cccgcccttg agccccggca gcagccagag ctgcttgccg
     2041 gtcgccgggt cgtaggcgtc gagctggttg ccccccatca caacgagctg cggccgctca
     2101 tcgaccgtga gcaagagcgg cgtggtgtag gcgtcgcact cttcggcgtc ggcccgggtc
     2161 atccgcggaa ttttccagcg gacctccccg ctggccaagt cgtgggccag caggtagctc
     2221 tcgaccggct tgtcctgcag atcggcgagc gaatcctgca tgcagatcga gatcaccagg
     2281 tcgccgaaca gcacggggct gttggcgtgc ccccaccaga tcgaatacgc gccgtattcc
     2341 tcctgcaggt tgtgccgcca gagccgctgg ccgtcaaagt cgtacgccgc caggtcgccg
     2401 ttgccgaaat gggcgacgac ggtcttgccg tcggtcacgg gcgaagggct cgccaggttg
     2461 tgaagctggt ggaatttctg cgtggaacgc ttcggggctt cgcgcgtcgc ctcgccgctg
     2521 ccgatggtct gcgtccagac gatctgcccg gtctttttgt cgatccgcat gaggacgagc
     2581 tggttgtcgg ccgtgtggct ggtgacgaag atcgcattgc cccagatggc cggcgtgctg
     2641 gttccccact ccggcagcgg gcatttccag gcgatgctgc ggctctcgtg ccagacgatc
     2701 ggcaggtcct tctccgggct gacgcccgtc ccctccggcc cgcgccactg cggccaatcg
     2761 gcggcgatgg ccaaggcctg gctggcgacg agcaggatag cggtaaaggc aatgcgagcg
     2821 ggattcatgg gcggctgggg agggtggtcc gagggcgatt tcgcggcgca ggttccgatt
     2881 atacctccat ccccgccaac caggttcccg cagactgggg gatcgagctg gtgcaagcca
     2941 attcggctgg acggattctg ccagatgcgc cgcggcagag cggattgacc gctgggcaac
     3001 aggctgccgg ctggccgccg gcaggaaagc aagcgcagta gagttggggc atgacagtca
     3061 ctcaagaaat gatcgacgcg ctcttcgagc cggagatggt ggagaccctc tgcgggcagc
     3121 ccaagcaggg gccggcggtt ctgcccggcg cggagcgcgg ctcggcgcga gttccctttc
     3181 gcgggcgggc cgtggcgatc gtctttccgc ccccgtcgtc accacaggcg gagccggtcg
     3241 aaagcgaagt ggtgacgacc gacatctcgc gcggtggctt gagcctcttg caccgccgcg
     3301 agctgcaccg cgggcagcaa gttcttctgc agcttacccg cggcacctgc acggtcgaag
     3361 tctgctggtg ctgccgcgtc tggcccggcc tgtacatcgc cggctgtcgc tttgcggaca
     3421 tgacgtttgc ggatgcggcg agctgatcca gatcgctctc ttacggcgcc ggctgcggca
     3481 tcgcgggatc agcggcctgc ggctcgatca cgccgccgtc ccactcgatg cggcactgtg
     3541 gcagcgcgtc ggccagcttt ttcaccccga cggcggtcac cttggtctgc ttcaggttaa
     3601 gcctggtcaa gccggccaag ccctcaagat gcttgaggcc ggagtccgta acttgcgtcc
     3661 cttgcaaccc tagccagttc aggctgctcc agtcctggag tatttccacg ccggcgtcgt
     3721 tggtcggtga cagatcgagg tacagcgtct tgagctgcag tccctggaga tgggccaatc
     3781 cagcgcccgt gactttcgtt ccattgaggt ccaggtattg cagctccttc atcccctgaa
     3841 ggtgctgcaa accgtcatcc gtcacggcag tcgcttccaa tccgagccag gtgaggctcg
     3901 tcagcccctg gaggtggctc aggccagcgt ctgagacgcg cgtgaaattc agattcaagc
     3961 gctccaggtt gtccaggttg gcgacgtgcg ccagcccagc gtcggaaagg
//
LOCUS       DASUGB010000835         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1480676, whole genome shotgun sequence.
ACCESSION   DASUGB010000835 DASUGB010000000
VERSION     DASUGB010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480676"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..>4159)
                     /locus_tag="VFV87_15860"
     CDS             complement(182..>4159)
                     /locus_tag="VFV87_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5105296.1"
                     /translation="NGVLRLSLEHYNRLQMHFFHESEGISLIYYEDQNYRWAAYATTR
                     EAGKAKPATWAITATDDDRCRRTELRHGGPIELRWQPAGEGPGELVLSRGDIVLVSAP
                     LSGPPTDVFFEGRAAFQGIELVRTQGGPSPLPPSPAALVIDRPADLPWKTLPAEAPPP
                     EFLPDGGVRLAVSDAKQRVHCLTPLPKAGLFEIVVELDDISPGAAVFASRDDDQVTEL
                     VRFFRNRRNNQLYAVYRGPDDACEDDFAPPTERPVPIAQPAAGITAHAHCWVKLLFGG
                     GVLKWWLSADGQHWAQPEYPHTAQPGKITSIGLQAVANRKDVQVTLRRIELRELAGLA
                     SLADAKLREQAPPLPHSPSLGHWLVEATSQKPVGVGSDEWLRACAVRTLGDRPASELS
                     HALLEALLDDAIARDLPLDRQLAALNDATRVALDFRDGGAMRIGLPRRYVELGLRSAD
                     EQGLPAWSSVRQASSSSPIGTPLVAPFDLERPIRWQCVQLAAAGDGAAAADWAQTLKL
                     FQQQRHSPLVEWLETSSRRDLPGQSSPDSISRLKEGWREPLIEELSKEAYNAMAELQA
                     VLESEAWDDAARLVTSLDPEAAPGVAPYVNDKSLLASLPVAVQLTLAEYPQLRESLGG
                     QFAALARLRIGQAMATGDAASVELATVQFAETEAAADAHQWLGDRALSSGWFVQAIAA
                     YERAMQIEPSLAAAVSPRARLAAAMLGRDEQKPVTQQVQFGELSMSAAEFEALVTEMR
                     TRSQSPGLASPPGITTRFAPPQPSGFEVQIRSRLDGPVGDKPQEEVGRRTNQFRVPWA
                     DRQIATTVEGDVLYVSNRFQVAAYNLTNGQRLWQSQPPPGPMQRAQDWAMIAMRPLIT
                     ADRIFVRQLYSPNPLLVCLEKSSGKLLWVAEPIEREYIVSDPVIVQGQLVALSTALLQ
                     DNQAILRWCAFDPLTGELLRQRDLVQLRSTWGKRACCEVSATEDGLVAVLGGLTLAAD
                     TRGGLRWLRKHSLLPADEDPRWVLQMYHPPVIDGQRMFVAQPGVRTVECLDRATGGKI
                     WMSVMPEVVGLAGLAQDRLIVRTETDVRALDPTTGNVQWRFPATDLHSFQLADDAAVL
                     LAQREPAPNQKDQWLVRLTWLDPATGHATATTVLPTLVDPDPRLGPLVNYKDRLFTFF
                     GRGQHDPNRDVVELIPKGQADRPVPRAYVDDPWRSRILPSLAEAAFARLGPDWLLLSG
                     QAGDRTGLVEDAHGVKNVLGIRSTGAWPIAVGREVTVPTMGQPRLRLKIANDSGQIWK
                     LEVRHGSQVLRADELTDEKFPDRWKTLEIDLSPVAGKSAWLQFRAQSTSGDHVLYIER
                     AELLF"
BASE COUNT          670 a         1401 c         1389 g          699 t
ORIGIN      
        1 ggggcgtttg ccgtcggccc gatctccggc ggcgcattca accccgccgt ggcggtcggc
       61 atcacgatca tgggaaagtc cgcgctggcc aacatctgga tcttcctcgt cgccaatttc
      121 gccgccggcg ccgccgcggc ctattggttc aaagggatcc atccggggga ggagtaatcc
      181 cctagaacag cagctcggcg cgctcgatgt aaaggacgtg atcgccgctg gtggattggg
      241 cgcgaaattg cagccaggcg ctcttgccgg cgacggggga gaggtcgatt tcgagggtct
      301 tccagcggtc tgggaatttt tcgtcggtca gctcgtcggc acgcaagacc tgagagccgt
      361 ggcggacttc gagtttccag atctgcccgg aatcgttagc gattttcagt cgcagtcgcg
      421 gctggcccat cgtgggaacc gtgacctcgc gtcccacggc aatgggccag gcgccggtgg
      481 accggatacc gaggacattc ttgacgccgt gggcgtcctc gaccaggccc gtgcggtcgc
      541 ccgcctggcc gctcaacagg agccaatcgg ggccgagacg cgcaaacgcg gcttccgcca
      601 gcgacggcaa aatccgggat cgccagggat cgtcgacgta ggcgcgcggg accgggcggt
      661 cggcctggcc cttcgggatc agctcgacga cgtcgcgatt gggatcgtgc tggccgcgac
      721 cgaagaaggt gaagaggcgg tccttgtagt tgaccagcgg ccccagccgc gggtcgggat
      781 cgacgagcgt ggggagcacc gtcgtcgccg tggcatgccc agtcgccggg tcgagccacg
      841 tcagccgcac gagccactgg tccttctgat tcggcgccgg ctcgcgctgg gcgagcagca
      901 cggcagcgtc atcggcgagc tgaaagctgt gcaggtcggt tgccggaaag cgccattgca
      961 cgttgccggt cgttgggtcg agcgcccgca catccgtctc ggtccgcacg atcaatcggt
     1021 cctgcgccag gccggcgagg ccgacgactt ctggcatcac gctcatccaa atcttccctc
     1081 ccgtcgcacg gtcgagacat tcgacggtgc ggacaccggg ctgagcgacg aacattcgct
     1141 ggccgtcgat gacgggtgga tggtacatct gcaggaccca gcgcggatct tcgtctgcgg
     1201 ggagcaggct gtgtttgcgg agccagcgga ggccaccacg tgtatcggcg gcgagcgtga
     1261 gcccaccgag caccgcgacc agtccatctt cagtcgccga gacctcgcag caggctcgct
     1321 tgccccacgt gctgcggagc tgaacgaggt ctcgctgtcg caagagttcg cccgtgagcg
     1381 ggtcgaacgc acaccaacgc aggattgcct ggttgtcttg cagtagcgcc gtggagagcg
     1441 ctacgagctg cccctgcacg atgaccggat cggagacgat gtactcccgc tcaatcggct
     1501 cagccaccca gagcagcttg ccggacgatt tctccagaca cacgagcagc ggattgggcg
     1561 aataaagctg ccgcacgaaa atgcggtcgg ccgtgatcag cggacgcatg gcgatcatcg
     1621 cccagtcctg ggctcgctgc atggggccgg ggggcggctg gctctgccag agacgctggc
     1681 cattggtcag gttgtaagcc gcgacttgga agcgattcga tacgtaaagc acgtcccctt
     1741 cgaccgtcgt tgcgatctgg cggtcggccc acggcacgcg gaactggttt gtgcggcggc
     1801 ccacttcctc ttgcggcttg tcgcccacag gcccatccag ccggctgcgg atttggacct
     1861 cgaagccgga gggctggggc ggcgcgaagc gagtcgttat tcctggcgga ctcgctagcc
     1921 ccggtgattg actgcgggtc cgcatttccg tcacgagcgc ctcgaattcc gcggctgaca
     1981 tgctcagctc gccgaactgc acttgctgcg tgacgggctt ttgctcgtcg cggcccagca
     2041 tggccgccgc cagccgcgcg cggggcgata ccgccgctgc cagtgacggc tcaatttgca
     2101 tggcccgctc gtacgcggca atcgcctgca cgaaccagcc gctcgatagg gcccggtcgc
     2161 ccagccactg gtgggcgtcg gctgcggcct ccgtttcggc gaattggacg gttgccagct
     2221 ccacgctcgc ggcatcgccc gtcgccatcg cctggccgat ccgcagcctg gccagcgcgg
     2281 caaactggcc ccccagcgat tcgcgaagct gcgggtattc ggcgagcgtg agctgcacgg
     2341 ccaccggcag cgaggcgagc agcgacttgt cattcacata cggcgccacg cccggcgcgg
     2401 cctccggatc gagcgaagtc accaaccgcg ccgcgtcatc ccaggcttcg ctttccagca
     2461 cggcctgcag ttcggccatc gcgttgtagg cctccttgct caactcttcg atcagcggtt
     2521 cacgccagcc ttccttgagg cggctgatcg agtcggggct cgactgaccc ggcaaatcgc
     2581 ggcgactgga cgtctccagc cattcgacga gcggcgagtg ccgctgctgc tggaagagtt
     2641 tcaacgtctg tgcccaatcg gctgctgcag ccccatctcc cgccgcggct agctgcacgc
     2701 attgccagcg aatcggccgc tccagatcga agggcgccac gagcggcgtg ccgatgggcg
     2761 atgaactgct cgcctggcgc acgctcgacc acgccggcag gccttgctcg tccgccgagc
     2821 gcagccccag ttcgacatat ctccgcggca agccgatgcg catggcgccc ccgtcgcgaa
     2881 aatccagggc cacgcgcgtc gcatcgttca gcgccgcgag ctgccgatcc agcggcaggt
     2941 cgcgggcaat cgcatcgtcg agcagcgctt cgagcaaggc gtggctcagc tcgctcgccg
     3001 ggcggtcgcc cagcgttcgc acggcgcacg cccgcagcca ctcgtctgag ccgacgccga
     3061 ccggtttctg gctcgtcgcc tccaccagcc aatgacctag cgatggcgag tgcggcaatg
     3121 gcggcgcctg ctcgcgcagc ttggcatcgg ccagcgaggc cagaccagcg agctcgcgca
     3181 gctcgattcg ccgaagtgta acctgcacgt ccttgcgatt ggccaccgcc tgcaacccga
     3241 tcgacgtgat cttgccaggt tgggccgtgt ggggatactc gggctgcgcc caatgctggc
     3301 cgtcagcact cagccaccac ttcagcacgc cgccgccaaa cagcagcttt acccagcaat
     3361 gcgcatgcgc agtgattccg gcggcgggct gcgcaatggg cacgggccgt tcggtcggcg
     3421 gcgcgaaatc gtcttcgcag gcatcgtccg gcccccgata gaccgcataa agctggttgt
     3481 tgcgacgatt gcggaagaag cggaccagct ccgtcacctg gtcatcgtcc cggctcgcga
     3541 aaaccgcggc gccgggcgag atgtcgtcca gttccacaac gatttcgaag agacccgcct
     3601 tcggcagcgg ggtcaagcaa tgcacccgct gcttcgcgtc gctgacggcc aatcgcactc
     3661 ctccgtcggg caggaattcc ggagggggcg cttcggccgg caaggtcttc cagggcaggt
     3721 cggcaggacg atcgatcacc aacgccgccg gactgggcgg caaggggctt ggtccgccct
     3781 gagtgcggac gagttcgatg ccttgaaacg ccgctcggcc ctcgaaaaac acgtcggtcg
     3841 gcgggccgga cagcggagcg ctgacgagga cgatgtcgcc gcggctgagg acgagctcgc
     3901 cgggtccttc gccggccggc tgccagcgca gctcgatcgg cccgccgtgg cgcagctccg
     3961 tccgccggca gcggtcgtcg tcggtcgccg tgatcgccca ggtcgcgggc ttggccttgc
     4021 cggcctcgcg ggtcgtggcg tacgcggccc agcggtaatt ctggtcctcg taatagatca
     4081 acgagatccc ttcgctctcg tgaaagaagt gcatctgcag gcggttgtag tgctcgagcg
     4141 acagccgcaa gacgccatt
//
LOCUS       DASUGB010000836         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1341802, whole genome shotgun sequence.
ACCESSION   DASUGB010000836 DASUGB010000000
VERSION     DASUGB010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341802"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..709)
                     /locus_tag="VFV87_15865"
     CDS             complement(29..709)
                     /locus_tag="VFV87_15865"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEX5105297.1"
                     /translation="MIVRRGALGLAILAGGLAAAEEVRPLETVQGWGLIVDPVGDCKF
                     EHAGGNFTIKVPGAHHDLWPVKGKVNAPLVLQEVAGEFTIDVLVEEVTQAEPDTVLPG
                     MASTASFHAGSLIIWQDAKNFVRFDRTNMNKAGRATTACYLHVYQNGERTAELASVVP
                     DRPTHLRLKRKGDLVTAAYSQDGAKTWTSLAEQKAKLPDKLKAGVSALNNTSRENAVR
                     FANLKLGK"
     gene            complement(715..1827)
                     /locus_tag="VFV87_15870"
     CDS             complement(715..1827)
                     /locus_tag="VFV87_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105298.1"
                     /translation="MATAVFAQAPGGAAAPAPGGTAADTAQVPKGYVGKWKGIRGKAT
                     ANSRGRIGTAIVNKDFGHVIDKFEFEVAEDGTITGTGTATYWFDITADADLVVTRTAP
                     EAHLEGKSQAVTFHITGKMVAGRAELTATPDEDLVLINAGKRTTMGAWNVFGPGSVLV
                     RKTDKGLEIGASRVIEQLGMKLEWEARKATCPNPLDVARDLAATKYDGWTYGSNAANE
                     QVDCVQFVLATVEKLHGQPLPRATRTRILISDVTSADVPVLVPANHSRTKGVQQALVD
                     IDHGTVVSIADVQAGDLVQYWMQKRDGSWMGHAAVVAEVLKDAAGTPDRIRIYGAQSS
                     DGGIALHDDPIRLSGGGRKMYLVRYKCESSQNCCQQ"
     gene            complement(1934..>2931)
                     /locus_tag="VFV87_15875"
     CDS             complement(1934..>2931)
                     /locus_tag="VFV87_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5105299.1"
                     /translation="IPTGLNVTFVIYGHEVFSSADDPRNCQAVKVARPLGKLDDAGKA
                     ELTDLVAGLKPTGGTPIALSLKVTGEELAKDKDAFCGIVLITDGLESCKGNPNEEVAT
                     LLKTLKLSFGVNLVGLGVKEAEDAALKTLAEAGGGKYYDADDADALADSISAIAKELE
                     VKAKPVEVVNTTRRSLKFLQPTIEMPEMKEIVLIEADGPVKDATLFKKGGITKYGEEI
                     RVPSSTAKYDVVWYPKSGEAIRIMKGVQLTERKVVDVKVEDLVGLIKVNGEGAPEIIH
                     AGAVDDPGDLSFSTQRAKKFGEVMVVPVGSYDVFVDRSVIEEGLKVEPGKLYELE"
BASE COUNT          493 a          973 c          846 g          619 t
ORIGIN      
        1 cggcacaacg attccgcgaa gcatcatctc actttccgag cttcaggttc gcgaagcgga
       61 cggcgttttc gcggctggtg ttgttgaggg ccgagacgcc tgccttgagc ttgtcgggca
      121 gcttggcctt ctgttcggcc aggctcgtcc aggtcttcgc gccgtcctgg ctataggcgg
      181 cggttacgag gtcgcccttg cgtttgagac gcaggtgcgt gggccgatcg ggcacgaccg
      241 aagccagttc cgcggtccgc tcgccatttt gatagacgtg cagatagcag gcggtcgtgg
      301 ccctcccggc tttgttcatg ttcgtgcggt cgaagcggac aaagtttttg gcatcttgcc
      361 agatgatcag cgagccggca tgaaacgacg cggtcgaggc catcccgggc agcaccgtat
      421 ccggctcggc ctgggtgact tcctcgacga gcacgtcgat cgtgaattcg ccggcgacct
      481 cttgcagcac gagcggcgca ttgacctttc ccttgacggg ccacagatcg tgatgcgctc
      541 ctggaacctt gatcgtgaaa ttaccgccag catgctcgaa cttgcagtcg cccaccggat
      601 cgacgatgag tccccagccc tgaaccgtct ccagcggccg gacttcttcg gcagcggcga
      661 gcccgccggc cagaatggcc aatccgagcg cgcctcgtct gacgatcatt gagttcattg
      721 ctggcaacaa ttctgcgagc tttcgcattt gtatcgcacg aggtacatct tgcgcccgcc
      781 tccgctgagg cggatcgggt catcgtgcag ggcaatgccg ccatccgacg attgtgcgcc
      841 gtagatgcgg atgcggtccg gagttccggc cgcgtccttc aacacttccg caacgaccgc
      901 cgcatgcccc atccacgagc cgtcgcgctt ttgcatccag tattggacga gatcgccggc
      961 ctggacgtcg gcgatcgaaa cgacggtgcc gtgatcaata tcaaccagcg cctgctgcac
     1021 gcccttggtg cgtgaatgat tggccggcac gagaaccggt acatccgcgc tggtgacatc
     1081 ggaaatcagg atccgcgtcc gcgtcgcccg cggcagcggc tgaccatgca gtttctcaac
     1141 cgtcgccaac acgaactgca cgcagtccac ctgctcattg gcggcattcg acccgtacgt
     1201 ccagccgtcg tacttcgtcg ccgccagatc cctggccacg tcgagcggat tcgggcaagt
     1261 cgccttgcga gcctcccatt ccagcttcat gcccagttgc tcaatgacgc gcgaggcgcc
     1321 gatttccaaa cccttgtctg tttttcgcac gagcacgctg ccgggcccga agacgttcca
     1381 agcccccatc gttgtgcgtt tgccggcatt gatgaggacc aggtcttcgt cgggagtcgc
     1441 cgtcagctcg gcccggcccg caaccatctt gccggtgata tggaacgtca ctgcttgcga
     1501 cttcccttcc agatgagcct cgggcgccgt gcgggtgacg accagatcgg catcagccgt
     1561 gatatcgaac cagtaggtgg ccgtaccggt gccggtgatc gttccatcct cggcgacctc
     1621 gaattcaaac ttgtcgatga cgtgaccgaa gtctttgttg acgatcgccg tgccgatccg
     1681 gccgcgcgag ttggccgtcg ccttgccccg aatgcccttc cacttgccga catagccctt
     1741 ggggacttga gcagtgtcgg ctgcggttcc acctggcgcg ggcgctgcgg cgccgcctgg
     1801 cgcttgagcg aacacggcag ttgccatcag cccactcaag gcggccgtca agaccgccgc
     1861 tctgcgaaga atcggaacgc tggtcattgc ctgatctccc gcgcgccacc cggcgatcct
     1921 tctgccaaga cgtttactcc agctcgtaca gcttgcccgg ctccactttg agcccctctt
     1981 cgatcacgct gcgatcgacg aacacatcgt agctaccgac ggggacgacc atcacttcgc
     2041 cgaatttctt ggcccgctgc gtcgagaacg acaagtcgcc cgggtcatcg acggcgccgg
     2101 cgtgaatgat ctcgggcgcg ccctcgccat tgaccttgat caggcccacc aggtcttcga
     2161 ccttgacatc caccaccttg cgttcggtca gttgcacacc cttcatgatg cgaatcgctt
     2221 cacccgactt cggataccag acgacgtcgt acttggccgt cgacgacgga acgcggattt
     2281 cctcaccgta cttggtgatc ccgcccttct tgaacagcgt ggcgtccttc accggcccat
     2341 cagcctcaat gagcacaatc tctttcattt ccggcatttc gatcgtgggc tgcaggaact
     2401 tgagcgaccg gcgcgtcgtg ttgacgactt ccaccggctt ggccttcact tccagctcct
     2461 tggcgatcgc ggaaatcgag tcggccaggg cgtccgcatc atcggcgtcg tagtacttgc
     2521 cgccgccggc ttcggcgagg gttttcaggg cggcgtcttc ggcttccttc acgccgaggc
     2581 cgaccaggtt cacgccgaag ctgagcttca gcgtcttgag caacgtcgcg acctcctcgt
     2641 tcgggttgcc tttgcagctt tccagtccat cggtgatgag cacgatgcca cagaaggcgt
     2701 ctttatcctt tgccagctct tcgccggtga ctttcagcga caacgcgatc ggcgtgccgc
     2761 cagtcggctt gaggccggcg actaagtcgg tcaactccgc tttgcccgcg tcatcgagct
     2821 tgcccagcgg gcgggcgacc ttcacagcct ggcaattccg cggatcatcc gcactgctga
     2881 acacctcatg gccatagatg acgaatgtca cgttcaagcc agtgggaatc t
//
LOCUS       DASUGB010000837         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1064696, whole genome shotgun sequence.
ACCESSION   DASUGB010000837 DASUGB010000000
VERSION     DASUGB010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064696"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFV87_15880"
     CDS             <1..627
                     /locus_tag="VFV87_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX5105300.1"
                     /translation="NPFMVLNELEAGLKHHSLITNEETKQEYRDLLGVVKEEYENIVK
                     NEVQRAIAADEDALKRLCGNYIDNVKAYTQREKVRNKFTGQFDEPDERLMRSIEEKID
                     IPDSRKDDFRREIMNYIAALLMDGKTFDYKSNERLYKALQLKLFEDQKDTIKLTSLVS
                     QVVDKDTQAKIDVVKSRLIRDFGYDDESATDVLNYVASIFARGDVKKK"
     gene            731..1834
                     /locus_tag="VFV87_15885"
     CDS             731..1834
                     /locus_tag="VFV87_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX5105301.1"
                     /translation="MKIDRDSSRFKQIVRGKIRDNLRKYVTHGEMIGRKGNDLVSIPL
                     PQLDVPHFRYGQNGRGGVGQGEGEVGQPIASGDPDGDGQGQAGSDPGQHVLEVDVTME
                     ELAAILGDELQLPRIEPKGKANIEQDKVRYTSVRRVGPESLRHFRRTYVQALKRQLSE
                     GTYRLNEPRVVPYKDDRRYRSWESVPEPVANAVIIYMMDVSGSMTDDQKEIVRTESFW
                     IDAWLRSQYQGLERRYIIHDAAAKEVDEHTFYHTRESGGTRISSAYKVCQGLLKNDFD
                     PAEWNIYCFQFSDGDNWGEDNKQALAMLREELMPACNLFCYGQVESPYGSGEFYRVLE
                     EAFGAEHETLVLSEIENREAIYDSIKKFLGKGK"
     gene            1986..3527
                     /locus_tag="VFV87_15890"
     CDS             1986..3527
                     /locus_tag="VFV87_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX5105302.1"
                     /translation="MPALHRFRSLTPEMADIQGQMEAHARSYGLDFFTTIFEVVDHDQ
                     LSAVAAYGGFPTRYPHWRFGMEYEQLSKGYSYGLQKIYELVINNDPCYAYLLSSNGIT
                     DHKLVMAHVYGHCDFFKNNAWFAPTNRRMIDEMANHGNRIRDYIDRFGVEEVETFIDA
                     CLSIEDLIDIHAPHIKRRDDKSKYEFKSTVEQEPEMPTATRFQAKGYMDSFVNPRQAL
                     DLELEEKKRELERKENFPSEPQRDIMLFLIEHAPLKPWQLDVLSIIRDEAYYFAPQAQ
                     TKIMNEGWATYWHSTIMTRHGMCDSDLICYADHCAGTLAGSRTRLNPYKLGVELFRDV
                     EDRWNRGAFGEEYDHCDDALAKRDWDKQLGKGREKIFEVRRIHNDLTFIDSFLTLDFC
                     RRHKLFSFGYNENSGYYEIESREFAKVKERLLFNLTNVGRPIIYVKDANYKNRGELFL
                     EHRFSGVELQTNYAQDTLANLYKLWKRPVHIETALEEAKVVLSFDGKEHTQTGGAAKQ
                     SPRGK"
     gene            3623..>3926
                     /locus_tag="VFV87_15895"
     CDS             3623..>3926
                     /locus_tag="VFV87_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105303.1"
                     /translation="MTITADIQRELKRIQAVSGRGLLQVDTDAGGRIEADLVAVDAIG
                     CSFQTLAHSTDKLAGADLEKLKEISEALTSRLTYLLEPIGLIEADVDRCAVQLRSSP"
BASE COUNT          902 a         1239 c         1142 g          643 t
ORIGIN      
        1 aacccgttca tggtgctcaa cgagctggaa gcgggcctca agcaccatag cctgatcacg
       61 aatgaagaaa cgaagcagga gtatcgcgac ttgctcggtg tggtcaagga agagtatgag
      121 aacatcgtga aaaacgaagt ccagcgggcg attgccgccg acgaagacgc cctgaagcgc
      181 ctctgcggca attacatcga caacgtgaag gcttacacac agcgcgaaaa ggttcgcaat
      241 aaatttacgg gccaattcga cgagccggac gagcgactga tgcggtcgat cgaggaaaag
      301 atcgacatcc ccgacagccg caaggacgac ttccgccgcg aaatcatgaa ctacatcgcc
      361 gccctgctca tggacggcaa gaccttcgac tacaagtcca acgaacggct ctataaggct
      421 ctgcagctca agctgttcga ggaccagaag gacacgatca agctgacgag cctcgtcagc
      481 caggtcgtcg acaaagacac ccaggccaag atcgacgtcg tgaagagccg cctgatccgc
      541 gacttcggct acgacgacga aagcgccacc gacgtgctga actacgtggc cagcatcttc
      601 gcgcgcggtg acgtgaagaa gaaataggag cgggggttcg gggttcggga ctcgggccta
      661 ggtgaacttg gcccgtcttt tgcagccccc tcgccccgag tccccagccc cgaaccccgt
      721 ttgcatggtc atgaaaatcg accgcgactc gtcgcggttc aaacaaatcg ttcgcgggaa
      781 gatacgagac aacctgcgaa aatacgtcac gcacggcgag atgatcggcc gcaaggggaa
      841 cgacctcgtt agcatccccc tgccgcagct cgatgtgccg cacttccgct acggccagaa
      901 tggccgcggc ggcgtcggcc agggggaggg cgaggtcggc cagccgatcg cctccggcga
      961 cccagatggc gacggccagg gccaggcggg cagtgatccg ggccagcacg tcctggaagt
     1021 ggatgtcacg atggaggagc tggccgcaat tctcggcgac gagctgcagc ttccgcggat
     1081 cgagcccaag ggcaaggcca acatcgagca ggacaaggtc cgctatacga gcgtccgccg
     1141 cgtcggcccc gagtcgctcc gccacttccg ccgcacctac gtccaggccc tcaagcggca
     1201 gctctccgaa ggcacgtacc ggctgaatga gccgcgggtc gtgccctata aggacgaccg
     1261 ccgctatcgc tcgtgggaga gcgtgcccga gcccgtcgcc aacgccgtca tcatctacat
     1321 gatggatgtc agcggctcga tgaccgacga ccagaaggaa atcgtccgca ccgagtcgtt
     1381 ctggattgat gcctggctcc gcagccaata ccagggcctg gagcggcggt acatcatcca
     1441 cgacgccgcc gccaaagaag tcgatgagca caccttctat cacacccgcg aaagcggcgg
     1501 cacgcggatc agctcggcct acaaggtctg ccagggcctg ctgaagaacg acttcgatcc
     1561 ggccgagtgg aacatctact gcttccagtt ctccgacggc gacaactggg gcgaagacaa
     1621 taagcaggcc ctggccatgc tccgcgagga gctgatgccg gcctgcaacc tgttctgcta
     1681 cggccaggtc gaaagtccct acggcagcgg cgagttctac cgcgtcctgg aagaagcctt
     1741 cggcgccgaa catgagaccc ttgtcctctc ggagatcgaa aaccgggagg cgatttacga
     1801 ttcgatcaag aagttcctgg gaaaaggaaa gtagtcattg gtcagtcacg ctggttccga
     1861 cgctccgcgt gggaacccaa gttccgacgc tctgcgtcgc ggaaaggacg cagagcgtcc
     1921 atgcgtgcgt gacgacgcag agcgtcgtca cgagcgaact gaccactgac cagtgacgac
     1981 tcaccatgcc cgccctccat cgtttccgat cgctgacgcc ggaaatggcc gacatccaag
     2041 gccagatgga agcccacgcg cgcagctacg gcctcgactt cttcaccacg attttcgagg
     2101 tcgtcgatca cgaccagctc agcgccgtcg ccgcctacgg cgggttcccg acgcgctatc
     2161 cgcactggcg attcggcatg gaatacgagc agctctccaa gggctattcg tacggcctgc
     2221 agaagatcta cgagctggtg atcaacaacg acccgtgcta cgcgtacttg ctgtccagca
     2281 acggtatcac cgatcacaag ttggtcatgg cccacgtcta tggccactgc gacttcttca
     2341 agaacaacgc ctggttcgcc cccaccaatc gtcgaatgat cgacgagatg gccaaccacg
     2401 gcaaccgcat ccgcgattac atcgaccggt tcggcgtgga ggaggtcgag acgtttatcg
     2461 acgcctgcct ctcgatcgag gatctgatcg acatccacgc cccgcacatc aagcgccgcg
     2521 acgacaagtc gaagtacgag ttcaagagca ccgtcgagca agagccggaa atgcccaccg
     2581 ccacgcggtt ccaggcgaag ggctacatgg attcgttcgt caacccgcgg caggccctcg
     2641 acctggagct ggaggaaaag aaaagagagc tggaaaggaa agagaatttc ccgtccgagc
     2701 cgcagcgcga catcatgctc tttctcatcg agcatgctcc gctcaagccc tggcagctcg
     2761 acgtcctctc gatcattcgc gacgaggcct attacttcgc tccacaggcg cagacgaaga
     2821 ttatgaacga aggctgggcc acctattggc acagcacgat catgacccgt cacggaatgt
     2881 gcgattcgga cctgatctgc tacgccgacc actgtgccgg cacgctcgcc ggctcgcgga
     2941 cgcgactcaa cccgtacaag ctgggggtcg agctcttccg cgatgtcgag gaccgctgga
     3001 accgcggcgc cttcggcgag gaatatgacc actgcgacga cgcgctcgcc aagcgcgact
     3061 gggataaaca gctcggcaaa ggccgcgaaa aaatcttcga agtccgccgc atccacaacg
     3121 acctgacctt catcgactcc ttcctgacgc tcgatttctg ccgccggcac aagctgtttt
     3181 cgttcggcta caacgagaac tccggctatt acgagatcga aagccgcgag ttcgccaagg
     3241 tcaaggagcg gctgctgttt aatctgacga acgtcggccg gccgatcatc tatgtgaagg
     3301 acgcaaacta caagaatcgg ggcgagctgt tcctggaaca tcgctttagc ggcgtcgagc
     3361 tgcagacgaa ctacgcccag gacacgctcg cgaacctgta caagctctgg aagcgtcccg
     3421 tccatatcga gaccgccctg gaagaggcca aggtcgtgct ctcgttcgac ggcaaggaac
     3481 acacgcaaac cgggggagca gcgaaacagt caccaagagg caaatgacca atgaccaaat
     3541 cccaatgacc aatggtcggc aggttctact gcattggtca tttgtgcttg gacatcggtc
     3601 attcgagaac agagagggaa ctatgaccat caccgccgac atccagcgag aactcaaacg
     3661 catccaggcc gtcagcggcc gcgggctgtt gcaggtcgat accgacgccg gtggccgcat
     3721 cgaagccgat ctcgtcgcgg tcgatgccat cggctgctca ttccagaccc tggcccattc
     3781 gaccgacaag ctggccggag ccgacctgga gaagctgaag gagatcagcg aggcgctcac
     3841 gtcgcgcctg acctacctcc tggagccgat cggtctgatc gaagccgacg tcgatcgctg
     3901 tgccgtgcag ctccgcagca gcccgc
//
LOCUS       DASUGB010000838         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_91465,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000838 DASUGB010000000
VERSION     DASUGB010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91465"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /gene="tnpA"
                     /locus_tag="VFV87_15900"
     CDS             complement(<1..352)
                     /gene="tnpA"
                     /locus_tag="VFV87_15900"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX5105304.1"
                     /translation="MAQSLAKLLVHLVYSTKHRARALPHQPYRELHAFCGGVLKNHKC
                     YLVEMNNVEDHVHALLDLHRTEALSDIVRELKTSSTKWLKEQSPDYSDFEWQDGFGAF
                     SLGMSQKPRAIGYLR"
     gene            complement(508..1095)
                     /locus_tag="VFV87_15905"
     CDS             complement(508..1095)
                     /locus_tag="VFV87_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5105305.1"
                     /translation="MPKKRILMLVGDYAEDYEVMVPYQMLLMVGHDVRAVCPGKEKGD
                     KIATSIHDFEGEQTYSEKRGHNFTLNATFDEIDLEAFDALVIPGGRAPEYLRLTPRVL
                     EIVRHFAAANKPIAAICHGVQLLSAAGVIKGRKISCYPACAHEVELAGGEYVPHASTY
                     DNAHTDGPFTTAPAWLAHPAWMRQFLAALGTEIEL"
     gene            complement(1196..1468)
                     /locus_tag="VFV87_15910"
     CDS             complement(1196..1468)
                     /locus_tag="VFV87_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105306.1"
                     /translation="MSLPTANNPVPVYNCVVHVAPPDAEGIVVARVANLPGLEGRGKT
                     EREALAAAVAAFKAAVAKVASGEKIPFIEPPQPPAGQAQRLIAVHL"
     gene            complement(1479..>2220)
                     /locus_tag="VFV87_15915"
     CDS             complement(1479..>2220)
                     /locus_tag="VFV87_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105307.1"
                     /translation="RAAHNEGMPPEDSAQTERAEREQRAEPEGRDSLDEPVDATVYDV
                     HPALVQQPAYPYGRPTARDTAPVDLTDPRGENPFRMREPESHRRQAVFSFPVAQPARY
                     KVPERFGMSAILGIMTALALLFGGLRLLDAYPAIYLFFGLQAVVICIVQMFYGKTPRA
                     ASAIAGAIILPAFILLGAMSERRGPPLSGVLCLMTMGVPIGAFLGYLTGTCAAGIFLV
                     MDALEPYLQGRRPVSLGTQPTAAPPVAS"
BASE COUNT          395 a          687 c          736 g          402 t
ORIGIN      
        1 gccgaaggta accaatggcc cgcggctttt gtgacatgcc gagcgagaat gcgccaaagc
       61 cgtcttgcca ctcaaaatcg ctgtaatccg gcgactgctc cttgagccac ttggtggagc
      121 tggttttcag ctccctgacg atgtccgaca gcgcctcggt gcgatgcaag tccaaaagcg
      181 catgcacgtg atcctcgaca ttgttcattt ccaccaagta gcatttgtga ttcttcagaa
      241 ctccgccgca gaaggcgtgc agctcccgat agggctggtg cggcagcgcc cgcgcgcgat
      301 gcttcgtgct gtaaaccaga tgcacgagca atttggccaa cgactgagcc atggccttct
      361 ccttgggccg tggcactctt gcagagtgcg aggcaaatga cgcgattcgt tacccagggc
      421 tgcgtttgct aaagcaaact tgccccgggc tgtcccgttt tggccttaca ggccggaaag
      481 ttcaagcacc gccgtctggc ttgcagttca cagctcaatc tccgttccca gcgccgccag
      541 gaactgacgc atccaggcgg gatgggcgag ccaggcgggg gcggtggtga acgggccgtc
      601 ggtgtgggcg ttgtcgtacg tgctggcgtg gggaacgtac tcgccgcccg ccaattccac
      661 ctcgtgggcg caggccggat agcaactgat cttgcgcccc ttgatcacgc cggcggccga
      721 caggagctgc acgccgtggc agatcgcggc gatcggcttg ttggcggcgg cgaagtgccg
      781 cacgatttcc agcacgcggg gcgtcagccg cagatactcc ggcgcccggc cgccgggaat
      841 gacgagggcg tcgaacgcct ccaggtcgat ctcgtcgaac gtggcattga gcgtgaaatt
      901 gtgcccgcgc ttctcgctat aggtctgctc gccctcaaaa tcgtggatgg aggtggcgat
      961 cttgtccccc ttctccttgc ccgggcaaac cgcccgcacg tcgtgcccca ccatcagcag
     1021 catctggtac ggcaccatga cttcgtagtc ttccgcatag tcgccaacga gcatgaggat
     1081 tcgcttcttg ggcatgaatg ggttcctctg tagggaacgc cctctgtggc gttccggaag
     1141 cgggtaggag ttcggatacg tgcggcacgc cacggagggc gtgccctaca gcgctttaca
     1201 aatgcaccgc gatcagccgc tgcgcttggc cggcgggcgg ttgcggcggt tcgatgaacg
     1261 gaattttctc gccagatgcc actttggcga cggctgcctt gaaggcggca acggcagcgg
     1321 ccagggcttc gcgctcggtt ttgccgcggc cttccaggcc ggggaggttg gcgacgcggg
     1381 cgacgacgat gccctcggca tccggcggcg cgacgtgaac cacgcagttg tatacgggga
     1441 ccggattgtt ggcagtgggg agcgacatgg ggcgtggatt acgacgctac gggaggagcg
     1501 gcggtcggct gggttcccag agacacgggc cgcctgcctt gcagatacgg ctccagggcg
     1561 tccatcacca ggaagatgcc ggcggcgcag gttccggtga ggtagccgag gaaagcgccg
     1621 atcggcacgc ccatcgtcat caggcacagg acgccgctca ggggcggccc gcgccgctcg
     1681 gacatcgccc ccagcaggat gaatgccggc agaataatcg ccccggcaat cgccgaggcg
     1741 gcccgcgggg tcttgccgta gaacatctgg acgatgcaga tcacgactgc ctgcagtccg
     1801 aagaacaagt agattgccgg ataggcatcc agcaggcgca agccgccgaa cagcaaagct
     1861 agcgcggtca tgattcccaa aatggccgac atgccgaacc gctcggggac cttgtagcgc
     1921 gccggctggg cgacgggaaa cgagaatact gcctgccgcc ggtgggactc gggttcccgc
     1981 atccgaaacg gattttcgcc gcgcggatcg gtcaaatcga ccggcgcagt atcccgggca
     2041 gttggacgac cgtacggata cgccggctgc tgaacgagag ccggatggac gtcatagacg
     2101 gtcgcatcga ccggctcgtc aagcgagtcg cgcccttcgg gctcggcccg ttgctcgcgc
     2161 tctgcgcgtt cggtttgtgc ggaatcttcc ggcggcatgc cctcattgtg cgccgcgcgg
//
LOCUS       DASUGB010000839         5892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1759652, whole genome shotgun sequence.
ACCESSION   DASUGB010000839 DASUGB010000000
VERSION     DASUGB010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759652"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..687)
                     /locus_tag="VFV87_15920"
     CDS             complement(34..687)
                     /locus_tag="VFV87_15920"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5105308.1"
                     /translation="MAPSQPSDFDQLRAAMVEQQLARRGIRDQRVLAAMRRVPREAFV
                     SAELAGTSYADSALPIDCEQTISQPVIVAMMTEALQLAGSERVLEIGTGSGYQTAILA
                     ELAAAVFSVERHAELSRQADVRLRKLGYENVFLKVGDGALGWPEEAPFDRIIVTAAAE
                     SCPPALWEQLVEGGVLVGPFGPAYDQALYETHKIGGQPQSRLLTGCRFVLLLPGVSE"
     gene            complement(772..1929)
                     /locus_tag="VFV87_15925"
     CDS             complement(772..1929)
                     /locus_tag="VFV87_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5105309.1"
                     /translation="MKIVCIHCGGEFSIKAEDLGGSGRCPHCKGEIALPKPSRPEADE
                     RPTRVRPLAWLDGSISGLASLVIHMTIFLIVALWQIDGGTGGAGEGEDVLIGLLPTQE
                     LSESPADELSAAEVQKEQSTDLDTMIEVEVPATAAVASSNGEISLSTPSPSGGEAGAF
                     DLGNVQIGGGSMGGGSWEGMIGQLRKSGLDIVLCFDSTGSMSGEINQVKRQIERIGQT
                     LTTLVPKARISICTYRDEGDEYVTKGLPLTGSIQDVSTYLGRVSAGGGGDHPEAVDEG
                     LYWSVSQNSFRPAARKVILLFGDAPPHPEKLKRAIEIAANFRTQNKGIVSTITCQSDT
                     PMKEFYEIAAAGGGEAFLTTDQRQIMTQLMVLVFGSRHRDKVIEAFKLLEE"
     gene            complement(2028..2615)
                     /locus_tag="VFV87_15930"
     CDS             complement(2028..2615)
                     /locus_tag="VFV87_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HEX5105310.1"
                     /translation="MHPVLSRNLFFIKEHVGFFKAANNYDVLDPESNQIIMNCREERL
                     GIFTKMLRFTDYKRLTPFEVIIRTPDGQPVICVKRGVALFLSKVDVLDENGQRVGGFA
                     QKFFSLFDRFTVNGANDQPLCTLKGNWTSWDFTFMAGDQELARVSKKWAGLGKELFTS
                     ADNYMLSISPVVPPDNPIRALILGAVMCIDLVLKE"
     gene            complement(2681..5011)
                     /locus_tag="VFV87_15935"
     CDS             complement(2681..5011)
                     /locus_tag="VFV87_15935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5105311.1"
                     /translation="MPALIRFATAGVVLVIFTAMANAEDTAETLRARAKAVLAQVEGQ
                     IKAPGLKAPVEVRRDRWGVPHIYAQNQHDLFFAQGYVAAQDRLFQIDLWRRVGVGQTA
                     EIFGEEGVEADRFARLVLYRGELEPEWKSYAPDTQEIATAFTDGINACIDQMQAQNKL
                     PIEFQLLGYQPQKWQPEDVLARMSGIIMTSNWQREVARAHLISRVGVERAREIAPTDP
                     PREFAPAPGLDLAAITPDIFQGYLAAARTLKFSPVTTESNNWAIDGTLSASGKPLLAS
                     DPHRPLGLPSLRYVVHLHAPGWNAIGAGEPGLPGIALGHNERIAWGITIVGTDQADLY
                     VEETKPDDPRQYKVGDSWQPMRIIRETIRVRGRTEPLELELRFTRHGPVIYQDESRGT
                     AIALQWAGSEPGGAAYLGGIAVARAQNHEQFLKALEGWHIPCLNFMYADVEGNIGWVA
                     AAHTPIRKNWDGLLPVPGASGDYEWQGFLPVSKLPQAFNPPQHWLATANHNILPPGYP
                     HQIAYEWAGPQRFQRVQTRLTEKKQFTLDDFQSIQHDSRSIPGLALAELAKHLDLPAD
                     LHPYRSILTGWDGVLSRDAQAGPLYAAWLQELLNAFYAESIPKDPRLERADLRNVTTM
                     LGQLQGRDELLRTTFAAAVSRVKQLQGNDPAQWRWGKLHTASFTHAIAGLGPAYEQVF
                     NLGPVERTGDGHTPNNSRHDDSFQQVHGASYRQLFDLADWDRGLATSTPGQSGQLGSP
                     HYSDLLPLWAEGQYFPLAYSRAKVEAVTQYRLELQP"
     gene            5143..5628
                     /locus_tag="VFV87_15940"
     CDS             5143..5628
                     /locus_tag="VFV87_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105312.1"
                     /translation="MTDLITPEDFGAVNSSLRAIVFLWVNWSFQARHSKRTFDELLAR
                     WEHEMADVPIAAFAIDVSVQEGKTWEATRTWLQSQQQPVDELTFSGCGGLLWLRSGQV
                     VAHSMSASGYEQDELFAFTKSVFELDDIPVINSSKLSELASKYQPPQWWYEGDEENLF
                     D"
     gene            5651..>5892
                     /locus_tag="VFV87_15945"
     CDS             5651..>5892
                     /locus_tag="VFV87_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105313.1"
                     /translation="MPIALFSAFVAFAWLETTLAEEDAGTASAWGRVIEDDRAIKIET
                     EQLEAVVPKKNPKQWMTGIEKQSFLDKATGYRELGDG"
BASE COUNT         1017 a         1907 c         1879 g         1089 t
ORIGIN      
        1 agccgcaatt gactcccgac cccttctccg ccgctactcc gagacgcccg gtaagagcag
       61 cacgaagcgg cagccggtga ggaggcgcga ctggggctgg ccgccgattt tgtgcgtttc
      121 gtagagcgcc tggtcataag ccgggccaaa gggaccaacg agcaccccgc cttccacaag
      181 ctgctcccac aaggccggcg ggcagctttc cgccgcggcg gtgacgatga tccggtcgaa
      241 cggcgcttcc tccggccagc cgagcgcccc gtcgccgacc ttcagaaaca cattttcata
      301 gccgagcttc cgcaatcgta catcggcttg ccgagaaagc tccgcgtgcc gctcgacgct
      361 gaagacggcg gcagccaatt ccgccaggat cgccgtctgg tagccgctgc cggtgccgat
      421 ttcgagcacc cgctcgctgc cggcgagctg cagggcctcg gtcatcatcg ccacaatcac
      481 cggctggctg attgtctgct cgcagtcgat cggcagggcg gaatcggcgt aggaagtgcc
      541 ggccagctcg gccgaaacga acgcctcgcg cggcacgcgc cgcatcgccg ccagcacgcg
      601 ctggtcgcgg atgccgcggc gggcaagctg ctgctcgacc atggcggctc ggagctggtc
      661 gaaatcgctc ggctgggaag gtgccatgga taggttgttt aacgcggagc cggaggcgac
      721 gctcactccg caagtgctcg cctgcggctc gccgttaaac gacgcggcga gtcactcctc
      781 cagcagcttg aacgcctcga tcaccttgtc gcggtggcgg ctgccgaaga cgagcaccat
      841 gagctgggtc atgatctgcc gctggtcggt agtgaggaag gcctcgcccc caccggctgc
      901 ggcgatctcg tagaactcct tcatcggcgt atcgctctgg caggtgatcg tgctgacgat
      961 gcccttgttc tgcgtgcgaa aattggcggc gatctcgatg gcccgcttga gcttctcggg
     1021 atgtggggga gcgtcgccaa acagcaggat caccttgcgg gccgccgggc gaaagctgtt
     1081 ctggctgacc gaccaataga gcccttcatc gacggcttcg gggtggtcgc cgccgccgcc
     1141 ggccgaaacg cggccgaggt acgtgctcac gtcctggatg ctccccgtca gcggcagccc
     1201 cttggtgacg tattcgtccc cctcgtcgcg ataggtgcag atgctgatgc gggccttggg
     1261 gacgagcgtg gtgagcgtct ggccgatccg ctcgatctgc cgcttcacct ggttgatctc
     1321 gcccgacatg ctcccggtgc tgtcgaagca gaggacgatg tccaggccgc tcttgcgaag
     1381 ctggccaatc atcccttccc agcttccgcc ccccatcgag ccgccgccga tctgcacgtt
     1441 ccccaggtcg aatgcccccg cttctccgcc gctgggcgaa ggcgtgctga gcgagatttc
     1501 gccattggac gacgcgaccg cggctgtcgc cggcacctcg acctcgatca tggtatcgag
     1561 atcggtcgat tgctcctttt gcacttcggc cgcgctgagc tcgtcggcgg gcgactcgct
     1621 caactcctgc gtcggcaaga ggccaatcag cacgtcctcg ccttcgcccg cgccgccggt
     1681 cccgccatca atttgccaca gggcgacgat cagaaagatc gtcatgtgga tcacgagcga
     1741 cgccaggccg gagatcgagc cgtcgagcca ggccaggggg cggacgcggg ttgggcgctc
     1801 gtcggcttcc gggcgcgaag gcttggggag ggcgatttcc cccttgcaat gcggacagcg
     1861 tccgctgccg ccgaggtcct cggccttgat cgagaactcg ccgccgcagt ggatacagac
     1921 gatcttcatg gacgcacgcg aaggttggct ggccgccttc tattttcgcg tgattgggcg
     1981 tgggcgtcaa atctccttcc tgtcccctgt cccctgtcca ctgtccccta ctccttcagc
     2041 acgaggtcga tgcacatcac cgcgcccaga atcagggcgc ggatcgggtt gtcggggggg
     2101 acgacggggg agatcgagag catgtagttg tcggcgctgg tgaacagctc tttgcccagg
     2161 ccggcccact tcttgctcac gcgggccagc tcctggtcgc ccgccatgaa cgtgaaatcc
     2221 cagctcgtcc agttgccttt gagcgtacat agcggctggt cgttggcgcc gttgacggtg
     2281 aacctgtcga acagcgaaaa gaacttctgg gcaaagccgc cgacccgctg gccgttttcg
     2341 tccagcacat cgaccttcga caggaacagg gccacgccgc gcttcacgca aatcaccggc
     2401 tggccgtccg gcgtgcggat gatgacctcg aacggcgtca gccgcttgta gtcggtaaag
     2461 cgcagcatct tggtgaagat ccccagccgc tcctcgcggc agttcatgat gatctgattg
     2521 ctctccggat cgagaacgtc atagttgttg gccgccttga aaaagccgac gtgttccttg
     2581 atgaaaaaca aattgcggga cagcacgggg tgcatcggca gagtctccaa atcgtggtgc
     2641 cagaattcca agcgatcgcg acgggccgat tatgggcgcg ctaaggctgc aattccagcc
     2701 ggtactgggt tactgcttcg accttggctc gcgagtaggc gagggggaaa tactgccctt
     2761 cggcccagag cggcaggagg tcggaatagt gcggactgcc gagctggccg gattggccgg
     2821 gagtgctggt tgccaggccg cggtcccaat cggccaggtc gaagagctgc cggtacgacg
     2881 cgccgtggac ttgctggaag gaatcgtcat ggcgcgaatt gttcggcgta tggccgtcgc
     2941 ccgtccgctc gacggggccg aggttgaaca cctgctcgta agccgggccg aggccggcga
     3001 tcgcgtgcgt gaaggacgcg gtgtgcagct tgccccaccg ccactgggcc ggatcattgc
     3061 cctgaagctg cttcacgcgg ctgaccgcgg cagcaaaggt cgtccgcagc agttcatcgc
     3121 gcccctgaag ctggccgagc atcgtcgtaa cattccgcaa gtcggcccgc tccagccgcg
     3181 ggtccttagg aatgctttcg gcatagaagg cgttcaacag ttcttgcaac caggcggcgt
     3241 agagcgggcc ggcctgcgcg tcgcgtgaga ggacgccgtc ccagccggtc agaattgatc
     3301 ggtagggatg caagtccgcc ggcagatcga ggtgcttggc gagttccgcc agcgcgaggc
     3361 cgggaatcga ccggctgtcg tgctggatgc tctgaaagtc gtccagcgtg aactgcttct
     3421 tttccgtcag gcgcgtttgc acgcgctgga atcgctgcgg gccggcccat tcgtaggcga
     3481 tctggtgcgg ataacccggc ggcaggatgt tgtggttcgc cgtcgccagc caatgctgcg
     3541 gcggattgaa cgcctgcggc agcttgctca ccggcagaaa gccctgccat tcgtaatcgc
     3601 cgctggcgcc cggcacgggc aacaggccgt cccagttctt gcgaatcggc gtgtgggcgg
     3661 cggcgaccca gccgatgttg ccctccacgt cggcgtacat gaagttcagg caagggatgt
     3721 gccagccttc gagggccttc aggaactgct cgtgattctg cgcccgggcg accgcgatcc
     3781 cgccgaggta cgccgccccg cccggctcgc tgccggccca ttggagggcg atcgccgtgc
     3841 cgcgcgattc atcctgatag attaccggcc catgccgcgt gaaacgcagc tccagttcga
     3901 gcggctccgt tcgcccgcgg acgcggatcg tctcgcggat gatccgcatc ggctgccagg
     3961 aatcgccgac tttgtactgc cgcgggtcgt ccggcttcgt ctcttcgaca tatagatccg
     4021 cctggtcggt gccaacgatc gtgatccccc aggcaatccg ctcgttgtgg ccgagggcaa
     4081 tgcccggcag ccccggctct cccgcgccga tcgcattcca gccgggggcg tgcaggtgga
     4141 cgacgtaccg caagctcggc agccccagcg gccggtgcgg atcgctggcc agcagcggct
     4201 tgccgctggc cgagagcgtg ccatcgatcg cccagttgtt gctctcggtc gtgacgggcg
     4261 agaacttcag cgtcctagcc gccgccagat agccctggaa gatgtcgggc gtgatcgccg
     4321 ccaggtcgag ccccggcgcg ggagcgaact cgcgcggcgg atcggtgggc gcgatctcgc
     4381 gggccctttc cacgccgacg cgcgaaatca agtgcgcccg ggccacctcc cgctgccagt
     4441 tgctggtcat gatgatcccc gacatccgcg ccagcacatc ctccggctgc cacttctgcg
     4501 gctgatagcc cagaagctga aactcgatcg gcagcttgtt ctgggcctgc atctggtcga
     4561 tgcacgcatt gatgccgtcg gtaaatgccg tcgcaatctc ctgcgtatcg ggcgcatagc
     4621 tcttccactc cggttccaac tcgccgcgat acagcaccag ccgcgcgaac cggtccgcct
     4681 cgactccctc ctcgccaaag atctccgccg tctggcccac gccgacccgc cgccacagat
     4741 cgatctgaaa cagccgatcc tgcgccgcga cgtacccctg cgcgaagaac agatcgtgct
     4801 gattctgcgc gtagatatgc ggcacgcccc acctgtcccg ccgcacctcc accggcgcct
     4861 tcagccccgg cgccttgatc tggccctcga cctgcgcgag gacggccttg gcccgcgcac
     4921 ggagcgtttc cgcagtgtcc tcggcatttg ccatggccgt gaaaatcacc aggacgactc
     4981 ccgcagttgc gaaacgaatg agcgctggca tgacgaagtc cgggcaaagt tgcgactgag
     5041 catatcgcga ggtagtttcc gcgtgatggg gacgaaatgc aacagttggc gctcgtgggt
     5101 tttccgctgc ttagcgcgac gactagaatc gaggcaatga aagtgacgga cctgatcacg
     5161 ccggaagact tcggggcggt taactcgagc cttcgtgcga tcgttttctt gtgggtgaat
     5221 tggtcgttcc aggcgcgcca ctcaaaacgt accttcgacg aactcctcgc tcgttgggaa
     5281 catgaaatgg cggacgttcc catcgcggcc ttcgcaatcg atgtcagcgt tcaggagggc
     5341 aaaacgtggg aagcgacgcg gacatggctc caatcgcagc agcaaccggt cgatgagctg
     5401 acattcagcg gctgcggggg actcctttgg ctccggtcgg gtcaggtcgt cgcccactcg
     5461 atgagcgcca gcggatacga acaggacgag ctattcgcct tcacgaaatc agttttcgaa
     5521 ctggacgaca ttccggtgat caattccagt aagctgagcg aactcgcttc caagtaccag
     5581 ccgccccaat ggtggtacga aggtgatgag gagaatctct ttgactaatt ccaccgacct
     5641 ccgccactca ttgcctattg cgcttttcag cgcttttgtc gcattcgcat ggctcgaaac
     5701 gacgcttgcc gaggaagacg cgggtaccgc ttccgcctgg ggccgagtca tcgaagacga
     5761 ccgggcgatc aagatcgaaa ccgagcagct cgaagcggtg gtgccgaaga agaatcccaa
     5821 gcagtggatg accgggatcg agaagcagtc gtttctcgat aaggcgacgg gctatcgcga
     5881 gttgggcgat gg
//
LOCUS       DASUGB010000840         8641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_91544,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000840 DASUGB010000000
VERSION     DASUGB010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91544"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1304
                     /locus_tag="VFV87_15950"
     CDS             81..1304
                     /locus_tag="VFV87_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105314.1"
                     /translation="MRKCLNVKKLLPIPAVVAAFLGACFLAPPPAQARVKLITLPVRE
                     RVEIQLDNAAATLVEEERIVPLVKGENQVDFSWANTQIDPNTIVFRVVAPVGEKMLDV
                     KVLSVSYPPNEAALIWSVGSSDSGSARVRISYVLGNLTKSFNYRAVASKDEKTLVLNE
                     YMRLQNFANEEFGDTNLFAGYGKTFLKPVGLNETKEMLVQKFDKVPIKKTYTCNVAEF
                     GWLDQPQNKLRVPMHYVLKNDKANQLGAHPLPYGKVRIFIDSGGESGTAFLGEDWGRF
                     TPIDDEMKLYLGVAQDIVVKRTIERNERKHVSGNLFHQEVILKYEIENFKDQAVTLDV
                     VENLRHVRNEVRGDSGRDVEWEILKETTFPGGLDKEKSTYEQVTFHAPLPARGKDGKA
                     EKITHKLHVLIKNEW"
     gene            1355..2758
                     /locus_tag="VFV87_15955"
     CDS             1355..2758
                     /locus_tag="VFV87_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX5105315.1"
                     /translation="MRTLICSLLVLVAGAIRAENIDLSTVPTRDAVQLTIYNSEDITL
                     VRETRKVTFKKGANPLQFSWANTLIDPTSVELKFLTQADKLVVLDTTFPHAKPQMLYW
                     NVNSEIDGEATIQITYFTSGITWTADYIVIADADEKNVKLDGFVRVHNNSGEEYEDAQ
                     VRLVVGTINLVEKIAQLAQMPVEEVLKLKNQELAERKKEATYGFMQRAGAMGGMGGGQ
                     TDAKEIVKEGLSEYFIFTIEGTETVPNGWSKRLRSIEAAAAPIKIQYRYRPNEYGDQL
                     VRMYLLTNDEKSQLGTAPLPDGIVRVFRDNGREGLSYLTQQQIKYIPIGDKIELNLGP
                     DPEVIFELIKLRAFRDELWLQINGVRELRKVGGDVVVKQENASIAGWDDHEVYSQRIR
                     NYTNKEIEVEVRRTFPGHVIFKSQLEPTLFDYQTPQFLAKIPAGKRSDLLFEIVRKQG
                     TSAKQNNVTLQSAEVLP"
     gene            complement(2888..2969)
                     /locus_tag="VFV87_15960"
     tRNA            complement(2888..2969)
                     /locus_tag="VFV87_15960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2933..2935),aa:Leu,seq:tag)
     gene            complement(3412..5550)
                     /locus_tag="VFV87_15965"
     CDS             complement(3412..5550)
                     /locus_tag="VFV87_15965"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5105316.1"
                     /translation="MSLPRGAGILPAVMFAALALPARAELPFPRFDRLQPLGLAAGGS
                     VEVEINGADIEGLSALRFDQLGLSAEPIAGKERWFKITAAPDVPAGTYDVRLVGRFGV
                     SSSKLLAVQHGLAEVAEPEPNNEPSQAQAVQVNCVINGSSDGENVDLYRFTAKAGQRI
                     VLDVDAQKLDSMLDSQLVVLNAAGKQLASCGDYHGRDSLIDFIVPADGEYIAVLHDLS
                     YRGGYPYRLTISDRPHVENVFPRAVQRGQTAQLTAYGRNLGGGPAAVGPTGEPALEST
                     SFGVMADVPPGQYRFLEHPTDHSVLPTAATFRLEGMQVRPSFPAGESLTAVTLLVVDE
                     PVTLEAEPNDKQDSPQSINLPAVVSGRFDQPRDADWFEFTASENGQYAVEIAAERLAA
                     QADSYFVLFDEQGNRQQEFDDYGHRINAFDGHLRDPVGNVNMQAGKKYRLLVLDRYQR
                     GGPRFQYVLTIRKAVPEAFVGVIHRENPTPAGLNLWRGGSTYLDLVIHHRDGNSTPVT
                     ISVKGPPPGVHFLPTTIPSDSRGAFVLWTDDNAPDFLGAIELVATYESGGQKIERPVR
                     SYTRVWNDAKGSSRPTRDLAAAVVEKAPFLVVPDQERVEVKAGDKLELKVQIKRLWPE
                     FTGDLKLLPLSWPGNFQMPELPVAAGASEATVAITVQNGTAPNEYTLALLAQAQVPFH
                     KDPAAKERPNTLVSLASRPITIVVQPAVKP"
     gene            complement(5547..6881)
                     /locus_tag="VFV87_15970"
     CDS             complement(5547..6881)
                     /locus_tag="VFV87_15970"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105317.1"
                     /translation="MLNLIDTGIRHCDGISRRAVMKIGALGLGGLSLPGLLRHEALAA
                     EATGSRPRRKSIILLWLAGGPSHIDMYDLKPDAPAEFRGEFQPIGTNVPGIDISEHLP
                     LQAKIMDKLALVRSAFHTNAGHGMGSQWMLTGWQPTIEVSDNIFPSCGSVVSKMLGPN
                     EPGLPAYVNLPRQIGLGKAAYLGASYNPFSPEDDPNNDGFSVKNLKIPGRVGTDRLAR
                     RQQLLGQIDQIRRDIDTKGDLTGIDTFYRDALEMVTSDKAQKAFHIQSEDPKLREKYG
                     RNDLGQSCLLARRLVEAGVTYVTVQAGGGWDTHGDNFSQLKKNLLPKYDQALTALVSD
                     LYDRGLSNDVLVMTMGEFGRTPRINGGAGRDHWPGAMTVVYAGGGLKMGQAIGTTNDK
                     AEYPATKAYTVGCVLSTMYKALGIDYKHAFYDQAQRPLPILNEGHPIEELVG"
     gene            complement(6973..>8641)
                     /locus_tag="VFV87_15975"
     CDS             complement(6973..>8641)
                     /locus_tag="VFV87_15975"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5105318.1"
                     /translation="VTRQCNFSSNLDVVARVDDLGLVSTAENRGEAAIMARYMGQVAV
                     FTALAPHGEPLAEIPGFVPRNYVDQLCAEKWKRLGLVPSPPCDDATFIRRVTVDLCGR
                     LPTADEVRAFLADSSSDKRTQLVDRLLDSPDYPAYFAMRWGTILRNSQLAGANQASYA
                     FHNWIKDSIAKNQPYDQFVRGIVAASGEWQDAPAINWLWQNRDDQLHQTTADVAQVFL
                     GTRLQCARCHHHPYERFSQEDYYGLAGFFSRMGRKSFGEPPPYFASPNVQTGEKNPLT
                     DKTPEPKYLDGEYAKFSPEEDPRHALVDWMARPDNPYFAKALVNRLWGHFFGRGIVHE
                     VDDLRQTNPPSNQELLDALARDFIEHKFDVKHIIQKLLTSQVYQLSAEPTPQNEHDQQ
                     NFARYYARRMPAEVLLDALDQATGTKTRFSGVAASSRAVDLPHEGFGSYFLDAFDRPR
                     RVTTCECERSSGATLAQVLLLANSDEIENKLADGNGKIAKLVETKRPTKEIVEELFLG
                     AFSRPPRDAELTAALATASAAGQNNERQALEDVLWTLINSREFMFNH"
BASE COUNT         1661 a         2617 c         2774 g         1589 t
ORIGIN      
        1 ggcgaaaacg aaacccccgc ctccgccgtc ttgattcttg tcgcgagaaa ccgaatctcc
       61 ctgccatgcg gagctatccg atgcgtaagt gcttgaatgt caagaagttg ctgccgattc
      121 ctgccgttgt tgcggcgttc ctgggtgcgt gcttcctggc tccgccgccg gcccaagccc
      181 gggtcaagtt gatcaccttg cccgtccggg aacgcgtgga aatccaactc gataacgccg
      241 cggcgacgct cgtcgaagaa gagcggatcg tgcccctggt gaagggggag aaccaggtcg
      301 atttttcgtg ggccaacacg cagatcgatc cgaacacgat cgtgttccgc gttgtcgccc
      361 ccgtcggaga gaagatgctc gacgtaaagg tcctcagcgt cagctatccg ccgaacgaag
      421 ccgccctaat ctggtcggtc ggcagcagcg acagcggatc ggctcgcgtg cggatcagct
      481 acgtcctcgg caacttgacc aaaagcttca actaccgggc cgtcgccagc aaggacgaga
      541 aaacactcgt cctgaacgaa tacatgcggc tgcagaactt cgccaacgag gaattcggcg
      601 acaccaacct cttcgccggc tacggcaaga cgtttctcaa gccggtcggc ctgaacgaga
      661 cgaaggagat gctcgttcag aagttcgaca aagtgccaat caagaagact tatacgtgca
      721 acgtcgccga gttcggctgg ctcgatcagc cgcaaaacaa gctccgcgtg ccgatgcact
      781 acgtcctgaa gaacgacaag gcgaaccagc ttggcgcgca tccgctgccg tacggcaagg
      841 tgcggatctt catcgacagc ggcggtgaaa gcggcaccgc gttcttgggc gaggactggg
      901 ggcggttcac gcccatcgac gacgagatga agctctatct gggcgtggcc caggacatcg
      961 tcgtcaagcg gacgatcgag cgtaacgagc gcaagcacgt cagcggcaac ctcttccacc
     1021 aggaagtgat cctgaaatac gagatcgaaa acttcaagga ccaggcggtg acgctcgatg
     1081 tcgtcgagaa cctgcggcac gtgcggaacg aagttcgcgg cgacagtggc cgtgatgtgg
     1141 aatgggaaat cctcaaggaa accaccttcc ccggcggtct cgacaaagaa aagagcacct
     1201 acgagcaggt cacgttccac gccccgctgc ctgcccgggg caaggacggc aaggcggaga
     1261 agatcacgca caagctgcat gtgctgatta agaatgagtg gtgatgtcag ttgtcagttg
     1321 tcagtggtca gttgggtgag gggtaggaga agctatgcgt acgttgatat gtagtttatt
     1381 ggtgctcgtc gcgggcgcta ttcgtgcgga gaacattgat ctttccaccg tgccgacgcg
     1441 cgatgcggtc cagctcacga tctacaacag tgaggacatc acgctggtcc gcgagacgcg
     1501 aaaggtgacg tttaagaagg gggcgaatcc gctgcagttc tcgtgggcca atacgctcat
     1561 cgatcccacg agcgtggaac tcaagtttct gacgcaagct gacaagctag ttgtcctcga
     1621 caccaccttt ccccatgcca agccgcagat gctgtactgg aacgtaaaca gcgagatcga
     1681 cggcgaagcg acgatccaga tcacctattt cacgagcggc atcacctgga ccgcggacta
     1741 catcgtgatc gccgacgcgg acgagaagaa cgtcaagctc gacggcttcg tccgcgttca
     1801 caacaacagc ggtgaagaat atgaagacgc gcaggtgcgg ctggtcgtgg ggacgatcaa
     1861 cctggtcgag aaaatcgccc agctcgccca gatgccggtc gaggaagtcc tcaagctgaa
     1921 gaaccaggag ttggccgagc gaaaaaagga ggcaacctat ggattcatgc agcgagcggg
     1981 ggcgatggga ggaatgggcg gcggccagac ggacgccaag gaaatcgtca aggaaggcct
     2041 cagcgagtac ttcatcttca cgatcgaagg gaccgagacg gttcccaatg gctggtcgaa
     2101 gcgtctccgt tcgatcgaag cagccgcggc cccaatcaag atccagtacc gctaccgccc
     2161 caacgaatac ggcgatcagc ttgtccggat gtacctgctg accaacgatg agaagtcaca
     2221 gctcggcacc gcgccgttgc cggacggcat cgtgcgggtc ttccgcgata acggccgcga
     2281 agggctgtcg tacctcacgc agcagcagat caagtacatc ccgatcggcg acaagattga
     2341 attgaacctg gggcccgatc ccgaagtaat cttcgagctg atcaagctcc gcgccttccg
     2401 cgacgagctc tggctgcaga tcaacggcgt ccgcgagctg cgcaaagtcg gcggtgacgt
     2461 ggtcgtcaag caagagaacg cctcgatcgc cggctgggac gaccacgagg tctatagtca
     2521 gcggatccga aactacacga acaaggagat cgaggtcgaa gttcgccgca ctttccccgg
     2581 ccacgtgatc ttcaagagcc agctcgagcc gacgctcttc gactatcaaa cgccgcagtt
     2641 cctggccaag attcccgccg gtaagcggtc cgacctgctc tttgagatcg ttcgcaagca
     2701 agggacgagt gccaagcaga acaacgtcac gctgcaatct gctgaggtgc tgccttgagc
     2761 ggcaactctt tgtcgcgatc tcctatggcg acgatggcag aattgtaacc tgcggtcttg
     2821 aacgtaccgc aacttgctcc ggcatcaaac tcggaatgct tgtgccatcg cgcggccgtt
     2881 caccgaatgc gagagacagg acttgaacct gcacgccttg cggcactgga tcctaagtcc
     2941 agcgcgtctg ccaattccgc cactctcgca agtaattaac cgacttgagt ttgcgtcaac
     3001 tggttcgcat cgtcaagcgg atattttggg gcccggaatc caaggggcgt gcatagaact
     3061 gtgaaccggt gaaattgtgg gcccggtcga tcaccagcac actggctacc ctttcggcct
     3121 gctcgcaccc tctaaggact aatgagcagc caagattgtc gctgccgttg ccaagggcgg
     3181 agcgaactcg atcgagggcc gagaattccg gctgacggac gtaaccggag aggtggtccg
     3241 accggtcctg gcgtaggcgg ggcacggtgg attcgcgcag tgcttggccg ggccgccact
     3301 tgcagagcgc ttcgatcaga acgccagtgc tcggtttgcc tcagcgtccg ttcgatcagc
     3361 cccaagcgcc tcgggaggca caccggtcgg gccggcggca tcagccgaag gttagggctt
     3421 gactgcaggc tgaacgacga tcgtgatcgg acggctggcg agcgaaacga gcgtattggg
     3481 acgctccttc gcggcagggt ccttgtggaa ggggacctgc gcctgggcca ggagcgcgag
     3541 cgtgtactca ttgggcgcgg tgccgttctg gacggtgatc gcaaccgtcg cttcactggc
     3601 gccggcggcg acgggcaact ccggcatctg gaagttgccc ggccacgaaa gcggcagcag
     3661 cttgaggtcg ccggtgaatt ccggccacag ccgcttgatc tggactttca attccagctt
     3721 gtcgccggcc ttcacttcca cgcgttcttg gtccggcacg accaagaaag gggccttctc
     3781 gacgaccgcc gccgccaggt cgcgcgtcgg ccggctcgat cctttggcgt cgttccagac
     3841 gcgcgtgtag ctccgcaccg gccgctcgat tttttggcca ccgctttcgt aggtggcaac
     3901 cagctcgatc gccccgagaa aatccggggc attgtcatcg gtccacagga cgaaagcccc
     3961 gcgcgaatcg gagggaatgg tcgtcggcag gaaatggacg ccgggcggcg ggcccttcac
     4021 gctgatcgtc acgggggtgg agttcccgtc gcggtggtga atcaccaggt ccaaatatgt
     4081 cgaaccgccg cgccacaggt tcaggccggc cggcgtggga ttctcgcggt ggatcacgcc
     4141 gacaaacgcc tcgggcacgg ccttgcgaat cgtcagcaca tactgaaatc gcggcccgcc
     4201 ccgctgataa cggtctagaa ccagcaggcg atatttcttg cccgcctgca tgttcacgtt
     4261 gccaaccgga tcgcggaggt ggccgtcgaa cgcattgatc cgatgaccgt agtcgtcgaa
     4321 ttcctgctgg cgattgccct gctcgtcgaa caggacgaaa taggagtcgg cctgcgccgc
     4381 aagccgctcg gcagcgatct ccacggcgta ctggccgttt tcgctggcgg tgaattcgaa
     4441 ccagtcggcg tcccgcggct gatcgaaccg gccgctgacg accgccggca ggttgatcga
     4501 ttgcggagaa tcctgcttgt cgttcggctc ggcttcgagc gtgaccggct catcgacgac
     4561 cagcagcgtc acggccgtca gcgattcgcc cgccggaaat gagggccgca cctgcatccc
     4621 ctccaggcgg aacgtcgccg cggtcggcag aacgctgtga tcggtcggat gctcaagaaa
     4681 tcggtactga cccggcggga catcggccat gacgccgaag cttgtcgatt cgagcgccgg
     4741 ctcgccggtc ggaccgacgg ctgccggccc gccacccagg ttccggccat aagccgtgag
     4801 ctgggccgtc tgcccgcgct gcacggcccg ggggaacacg ttttcgacat gcggccgatc
     4861 gctgatcgtc agccggtagg gatagccgcc gcgatacgac aggtcgtgga gcaccgcgat
     4921 gtattcgccg tccgccggga cgataaagtc gatcaacgaa tcgcggccgt gataatcgcc
     4981 acaggaagcg agctgcttgc cggcggcatt cagcacgacc aattgcgaat cgagcatcga
     5041 atcgagcttc tgggcatcga catccaggac gatccgctgg ccagctttcg cggtgaagcg
     5101 gtagagatcg acgttctcgc cgtcggacga gccgttgatc acgcaattga cctgcacggc
     5161 ctgggcctgg gacggctcgt tgttgggctc cggctcggcg acttcggcca ggccgtgctg
     5221 gaccgcgagc agcttggagc tgctgacgcc gaaccggccg acgagccgca cgtcgtaagt
     5281 tcccgccgga acatccggtg cggcggtgat cttgaaccag cgctccttgc cggcaatcgg
     5341 ctcggccgag aggccgagct gatcgaaacg caacgcggag agaccttcga tatccgcccc
     5401 attgatctcg acctccacgc tgccgccggc ggcgaggccc agcggctgga ggcggtcgaa
     5461 gcgggggaag gggagctcgg cgcgggcggg cagcgcgagc gcggcaaaca ttacggcagg
     5521 caggatgcct gccccacggg ggaggctcat ccgacgagtt cctcgatcgg atggccttcg
     5581 ttcaggatcg gcagcgggcg ctgggcctgg tcgtagaagg cgtgcttgta atcgatgccg
     5641 agggccttgt acatcgtcga aaggacgcag ccgacggtgt aggccttggt tgccggatac
     5701 tcggccttgt cgttggttgt gccgatggcc tgccccattt tcaggcctcc gccggcatag
     5761 acgacggtca tcgcgccggg ccagtggtcg cggcccgccc cgccgttgat tcgcggcgtc
     5821 cggccgaact cgcccatcgt catcacgagc acatcgttcg agagcccgcg gtcgtacaag
     5881 tcgctgacga gggcggtcag cgcctggtcg tacttgggca gcaggttctt cttgagctgc
     5941 gagaagttgt cgccgtgggt atcccagccg ccgccggctt gcacggtgac gtacgtgaca
     6001 cccgcctcga ccagccggcg ggcgaggagg cagctttggc ccaggtcgtt gcggccatac
     6061 ttttcgcgaa gcttgggatc ctcgctctga atgtggaatg ccttctgagc cttgtcgctg
     6121 gtgaccattt ccagggcgtc gcgatagaac gtgtcgatgc cggtcagatc gcctttcgtg
     6181 tcgatatcgc ggcgaatctg gtcgatctgg ccaagcaact gctggcggcg ggcgaggcga
     6241 tcggttccca cgcggccggg aatcttgagg ttcttcaccg agaagccgtc gttgtttggg
     6301 tcatcctcgg gcgagaatgg attgtacgaa gcgccgagat acgccgcttt gcccaggccg
     6361 atctgccgcg gcaggttcac ataggcgggc aggccgggct cgttggggcc aagcatcttg
     6421 ctcaccacgc tgccgcacga cgggaagatg ttgtcgctga cttcgatcgt cggctgccag
     6481 ccggtgagca tccactgcga gcccatgccg tggccggcgt tcgtatgaaa cgccgaacgg
     6541 acgagggcca gcttgtccat gatcttggcc tgcagcggca ggtgctcgga gatgtcaatc
     6601 cccggcacgt tggtgccgat cggctggaac tcgccgcgga attcggccgg ggcgtccggc
     6661 ttcaggtcgt acatgtcgat gtggctcggc ccgccggcca gccagagcag aatgatgctc
     6721 ttccgccgcg gccgcgagcc ggtcgcttcc gcagcgaggg cttcgtgtcg caagagaccc
     6781 ggcagcgaga ggccgccgag gccgagcgcg ccgatcttca ttaccgcgcg gcggctgatg
     6841 ccgtcacagt ggcggatgcc ggtgtcgatg aggttgagca tggctgtcgc tcccgtcttc
     6901 ataccaaccc gatgcgtaag cgagggagtg cgggcttgag gctgactacc tcgctgacgc
     6961 gtcgggttgg tgttaatgat tgaacatgaa ctcgcggctg ttgatcagcg tccagagcac
     7021 gtcttccagc gcctgccgct cgttattctg gccggcggca ctggcggtgg ccagcgcagc
     7081 ggtcaactcc gcatcgcggg gcgggcggga gaacgctccc aggaacagct cttctactat
     7141 ttccttcgtc ggccgtttcg tttctaccaa tttggcaatc ttgccgttgc cgtcagccag
     7201 cttgttctcg atttcgtcag aattggccaa taagagcacc tgggccagcg tggccccgct
     7261 cgatcgttcg cattcacagg tcgtcacgcg ccgcggccgg tcgaaggcat cgagaaaata
     7321 cgagccgaaa ccttcatgcg gcagatcgac ggcccggctg ctggcagcta cgccgctgaa
     7381 ccgcgttttc gtgccggtcg cctgatcgag ggcgtcgagc aatacctcgg ccggcatccg
     7441 ccgggcatag tagcgggcga agttctgctg gtcgtgctcg ttctgcgggg tcggttcggc
     7501 agaaagctga tagacctggc tggtgagcaa cttctggatg atgtgcttca cgtcgaactt
     7561 gtgttcgata aagtcgcggg ccagcgcgtc gagcaattcc tgattggagg gcggattcgt
     7621 ttgccgcagg tcatcgactt cgtggacgat cccgcggcca aagaaatggc cccacaggcg
     7681 attgacgagc gccttcgcga agtacggatt gtcgggcctg gccatccaat cgacgagtgc
     7741 gtgccgcgga tcttcttcgg gcgagaactt cgcgtactcg ccgtccagat acttcggctc
     7801 cggcgtcttg tcggtcagcg ggttcttctc gcccgtctgc acgttgggcg aagcgaaata
     7861 agggggtggc tcgccaaagc tcttgcggcc catccgcgag aagaagccgg ccaggccgta
     7921 ataatcttcc tggctgaacc gctcgtacgg atggtggtgg cagcgggcac attgcaatcg
     7981 cgtgccgaga aagacctggg ccacgtcggc cgtcgtctgg tggagctggt cgtcgcggtt
     8041 ttgccagagc cagttgatcg ccggcgcgtc ctgccattcg ccggaagccg ccacaatgcc
     8101 gcgcacgaac tggtcgtacg gctggttctt ggcgatcgaa tccttgatcc agttgtgaaa
     8161 ggcatagctc gcctggttcg ctccggcgag ctgcgaattg cggaggatcg tgccccagcg
     8221 catcgcgaaa taggccgggt agtcgggcga gtcgagcagc cgatcgacga gctgcgtgcg
     8281 tttgtccgac gacgaatcgg cgaggaaggc acggacctca tctgctgtcg gcaagcggcc
     8341 gcacagatcg acggtcacgc ggcggatgaa cgtcgcgtcg tcgcacggcg gcgaaggcac
     8401 gagccccagg cgcttccact tctcggcaca aagctgatcg acatagttgc gcggcacaaa
     8461 gccgggaatc tccgcgagcg gctccccgtg cggagccagc gcggtgaaga ccgcgacttg
     8521 tcccatgtac cgggccatga tggcggcttc gccgcggttt tcggcggtgc tcaccagacc
     8581 cagatcatcg acgcgggcca ccacatccag attgctcgaa aaattgcact gccgcgtcac
     8641 a
//
LOCUS       DASUGB010000841         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_787174, whole genome shotgun sequence.
ACCESSION   DASUGB010000841 DASUGB010000000
VERSION     DASUGB010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787174"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFV87_15980"
     CDS             <1..272
                     /locus_tag="VFV87_15980"
                     /inference="COORDINATES: protein motif:HMM:NF015440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-alpha domain-containing protein"
                     /protein_id="HEX5105319.1"
                     /translation="HIADLAKQVIVNGKCGWYLRVLVEGEIATGVPMDLGSRPHPAWT
                     VARASELFHHRKDDLAAAHELAALPELSAAWRESLHARLSKRRGG"
     gene            complement(234..1103)
                     /locus_tag="VFV87_15985"
     CDS             complement(234..1103)
                     /locus_tag="VFV87_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105320.1"
                     /translation="MPTIFALASGRSGTHFLSQLIRRNVDSRKAGGCVVRHEPYVFNP
                     SMFGRPIYDFAMGERQYTRTLLERKARIVQRCGAKTYVETSHAFLKSYFDLAPAYFDN
                     LKVIHLVRNPQQVARSEANREELTHWLHLPLRHYRGGDRRLYFRWSLTGLEPIFQHFD
                     LAELSLFQRYAIQWIEIENRAMQFLDRFSMHDRCFTLQTPHDLNDKQRVAELIEFLEL
                     PRRGEISLAGRRNLNPKPTVISDGDQRQFVEVIAALPRDRLEIFAHPPYDRWSWTSAL
                     TSAASPLAQPSVQ"
     gene            complement(1244..2089)
                     /locus_tag="VFV87_15990"
     CDS             complement(1244..2089)
                     /locus_tag="VFV87_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5105321.1"
                     /translation="MKYGMNLLLWTGELNDGMVPVLQMLKKQGYDGVELPIFNTGLDY
                     KKWGQVLDDLGLARTAVTVRTVDDNPISADADVRQKGIEGNCKVLDCCAAVGATHLVG
                     PFHSALGHFSGQGPTESEWKWGVESMRKVAEHAAKCKVTLGVECLNRFETYLLNTHAD
                     SARFCREVNHPNCKMMYDTFHAHIEEKSIPEAIRGCKDVLCHVHISENDRSTPGQGIV
                     RWDENWNTLREINYDGWLMVEAFGLALPELAAATKIWRRMFESEEQLSRDALAFMKTQ
                     VAKRW"
     gene            complement(2144..2821)
                     /locus_tag="VFV87_15995"
     CDS             complement(2144..2821)
                     /locus_tag="VFV87_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105322.1"
                     /translation="MNYLAHGWRFADQPYFLAGTAAPDWMSVIDRRNRLRGKVAERFV
                     EDPDPQVAALARGVVQHHADDAWFHQTTAFNELSLAFAVEIRDRLPGDEGFRPSFLGH
                     ILVELLLDAALAEEQPRRLDDYYAALRQLDPAATERAISKLAMQPTGRVAILVPRFIE
                     ERFLYDYREDGKLLTRLNHVMRRVGLPQLPAEIAEAFPSMRERVRTRMAELLAEPVQS
                     PKSKVQS"
     gene            complement(2903..2975)
                     /locus_tag="VFV87_16000"
     tRNA            complement(2903..2975)
                     /locus_tag="VFV87_16000"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2939..2941),aa:Glu,seq:ctc)
BASE COUNT          564 a         1040 c         1077 g          636 t
ORIGIN      
        1 ggcatatcgc cgatctggcg aagcaggtga tcgtgaatgg caaatgcggc tggtatttgc
       61 gcgtgcttgt cgagggcgag atcgccacgg gcgtgccgat ggacctcggg tcgcgtccgc
      121 atccggcttg gaccgtcgcg cgggccagcg agctgtttca tcatcgcaag gacgatctgg
      181 cagccgccca cgaactggcc gcactgccgg agctttccgc ggcgtggcgc gaatcactgc
      241 acgctcggct gagcaagcgg cgaggcggct gatgtcaggg cggatgtcca agaccacctg
      301 tcatagggcg ggtgggcaaa gatctcgagg cgatcccgcg gcaaggcagc gatcacctcc
      361 acgaactgcc gctggtcccc gtcgctgatg accgtcggct tggggttcag gttccggcgg
      421 ccggcgagcg aaatctcccc gcgccgcggc agttccagga actcgatcag ctcggcgacg
      481 cgctgtttgt cgttcaggtc gtgcggcgtc tgcagcgtga agcagcggtc gtgcatggag
      541 aagcggtcga ggaattgcat cgcccgattc tcgatttcga tccattggat ggcgtaccgt
      601 tgaaacagcg acaattctgc gagatcgaaa tgctggaaga tcggctccag cccggtgagc
      661 gaccagcgga agtacagccg gcgatcgccg ccgcggtagt gccgcagggg caagtgcagc
      721 cagtgggtca gctcctcgcg attggcttcg ctgcgggcga cttgctgcgg gttgcgcaca
      781 aggtggatga ccttcaggtt gtcgaaatac gccggggcca ggtcgaaata cgacttgaga
      841 aacgcgtggc tcgtttcgac gtaggtcttc gcgccgcagc gctggacgat gcgtgccttc
      901 cgctccagca gcgtgcgcgt gtactggcgc tcgcccatcg cgaagtcgta gatcggccgc
      961 ccgaacatcg acgggttgaa gacgtacggc tcgtggcgga cgacgcagcc gccggccttc
     1021 cgcgaatcga cgttgcggcg gatgagctgc gaaaggaagt gtgttcccga gcggccgctg
     1081 gcgagggcga agatggttgg cacgatcccg taggacgggt ttgtaacccg tccgttccgc
     1141 gttagcgatc ctcgttccca cgctccgcgt gggaacgcac gtcctgacgc tccgcgtcgt
     1201 tcgcgttgac tcggacgggc tggaagccca tcctacggag ccactaccac cgcttggcga
     1261 cctgggtttt catgaacgcc agggcgtcgc ggctgagctg ttcttcgctc tcgaacatcc
     1321 gccgccagat tttcgtggcg gcggccagtt ccggcagggc caggccgaac gcctcgacca
     1381 tcagccagcc gtcatagttg atctcgcgga gcgtgttcca gttttcgtcc cagcggacga
     1441 ttccctggcc gggcgtgctg cggtcgttct cgctgatgtg tacgtggcag agcacgtcct
     1501 tacagccgcg aattgcctcg gggatcgact tttcctcgat gtgggcgtgg aacgtgtcgt
     1561 acatcatctt gcagttcgga tggttcactt cgcggcagaa gcgggccgaa tcggcgtgcg
     1621 tgttgagcag gtacgtctca aagcggttga gacattcgac gccgagcgtc accttgcact
     1681 tcgctgcgtg ctcggcgact ttccgcatgc tctcgacgcc ccacttccac tcgctttcgg
     1741 ttggcccttg gccgctgaag tggccgagag ccgaatgaaa cggcccgacg aggtgcgtcg
     1801 cgccaacggc ggcacagcaa tcgagcactt tgcagtttcc ttcgatcccc ttctgtcgca
     1861 cgtcggcgtc ggcgctgatc ggattgtcat ccaccgtgcg aacggtgacg gctgtgcggg
     1921 cgaggcccaa gtcgtcgagc acctggcccc acttcttgta atcgaggccc gtgttgaaga
     1981 ttggcagctc gacgccgtcg tagccctgct tcttcagcat ctgcagcacc gggaccatgc
     2041 cgtcgttgag ctcgccggtc cagaggagca ggttcatgcc gtatttcatg tcgtttacct
     2101 cgactcgata tcgttgtggt ccaaagtcca aagtcgcgta gttctaactt tggaccttgg
     2161 actttggact ttggactggt tccgccaaca gctccgccat ccgcgttcgt acccgctccc
     2221 gcatcgacgg aaacgcctcg gcgatttccg ccggcaactg cggcaggccg acgcggcgca
     2281 tcacgtggtt cagccgcgtc agcagtttgc catcctcccg atagtcgtac aagaaccgtt
     2341 cctcgataaa tcgcgggacc agaatcgcta cgcggccggt cggctgcatg gccagcttgc
     2401 tgatggcccg ctcggtcgcc gcggggtcga gctgccgcag agccgcatag tagtcgtcga
     2461 gccgccgcgg ctgttcctcg gccaaagccg cgtcgagcaa cagctccacc agaatgtgcc
     2521 ccagaaagct cggccgaaaa ccttcatccc ccggcagccg gtcgcgaatc tccaccgcga
     2581 acgccaggct caactcgttg aatgcagtag tttgatgaaa ccaggcgtcg tccgcatgat
     2641 gctgcaccac tccccgcgcc agcgccgcca cctgtggatc aggatcctcg acgaaccgct
     2701 ccgccacctt cccgcggagg cggttccgcc ggtcgatcac gctcatccaa tcgggcgccg
     2761 ccgtccccgc caggaagtac ggctgatccg caaaccgcca gccgtgagcg aggtagttca
     2821 tggagccgcg atccgcatcg cccggccgag ggacaaaaaa accctcgcac caaggtgccg
     2881 caaggcaggg tgcgagggcg agtaccccct aggggagtcg aacccctgtt accggactga
     2941 gaacccgggg tcctaggcca ctagacgaag ggggcgggta cgtcactcca tttcagcatg
     3001 aaccagggag ttgtcaagtc ggcggaaggt tcgcggattg aagcgtggtc ggggtgattt
     3061 gcgaaatcgc acggcataca ctggggcagg ttgtgcgcga cattttgggc gggcttgctg
     3121 cctgcaggtc gtgcatcggg gcagccgtgt cacctatccc cagagcgtca gccgtgccgc
     3181 gagcgggttt tgtttcgtgc gatatcgtgg gccattccgc ggcgcgggat ctggccgtgc
     3241 aaaccgcgcg tctggcgacg atcaacgcgc tggtcaagtc cgcgctcgcg gctggcgagc
     3301 ccggccgcgt ggtatgg
//
LOCUS       DASUGB010000842         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1759856, whole genome shotgun sequence.
ACCESSION   DASUGB010000842 DASUGB010000000
VERSION     DASUGB010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759856"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFV87_16005"
     CDS             complement(<1..444)
                     /locus_tag="VFV87_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105323.1"
                     /translation="MSQIGVAVCPHCGARFGAAASVVGKQVPCPSCQKPFVVRIAPPA
                     PPAPAANPLDALSSAAPAGNPLAAPAGSALRPAVYPGQGGSQLPWLWIGGGVAAAVVL
                     VACVVGAWAALSRPGGDQDPVAAAAPAAAPSGLVPTKPASPPPPGP"
     gene            754..2025
                     /locus_tag="VFV87_16010"
     CDS             754..2025
                     /locus_tag="VFV87_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5105324.1"
                     /translation="MRTFIVILVVLAVLAVGGVVGYRPAVDAWRKHSMPKWRTAEVTE
                     GEIVETVNATGTVKPVLQVSVGSFVSGPILELYCEFNQEVKKDELLAKIDPRIYQAAV
                     DGGRANLLTRQADVKRVEAQLAQARRDENRAQELRQEDSAFIAQAEIDRVTYGRMALE
                     AQLDLAKAAVEQARFQLKRSEADLDYCEIRSPVDGIVINRKIDPGQTLAAQFQTPELF
                     IVAPDMRKKMHVHASVDEADIGLISTAQKKSLPVSFTVDAYPDEFSGTIEEIRLSSTT
                     TQNVVTYPVVVAAGNPDLKLLPGMTANISFEVDRCEEVLKIPNSALRFFPTPQHVRKE
                     DRPILEGKVAADEEQEQVASETLSARDRAEARRDRNRHHVWVVDGYELRAVEVITGLS
                     DSKYTQLVSGDLKAGDKLVTGIQPAMNGWGG"
     gene            2094..3335
                     /locus_tag="VFV87_16015"
     CDS             2094..3335
                     /locus_tag="VFV87_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105325.1"
                     /translation="MGFLLTLRIAFRALRKNKMRAGLTVLGVVIGIAAVTTMVSLGQS
                     ASQLVQDQLAGIGTNVMVVFPGNQERGGVRQGLVTTLTAADSDAIAEDCPSILASSPI
                     VGAQGQIIYGNSNYRPRDVLGVGKDFPIVRSWTLKRGGFFTEREIDSYDQVCVIGHTL
                     VRKLFQTSDPIDRTIRIDRVPFRVVGVLDAKGANMVGEDQDDIIVMPYTTVQKRLHGS
                     EFDNVNAIICSARSMEAMMEAKSEINQLLAERHRIPPGEPFDFTVATTIEMANMLGIV
                     LGTLTMMISSIAGISLVVGGVGIMNIMLVSVTERTREIGIRMAVGATSWDILRQFLVE
                     AILLSFIGGAIGFALGVAASVGVTMLINSLTTGTKWPIVISLPAAMLAIVFAAAVGVF
                     FGFYPARRASLLDPIEALRYE"
BASE COUNT          654 a         1023 c         1134 g          609 t
ORIGIN      
        1 cggacctggc gggggaggcg aagccggctt cgtcgggacg agtcccgacg gtgctgccgc
       61 aggcgccgca gcggcgacag gatcttgatc accgcccggg cgcgagagcg ccgcccaagc
      121 tccgacgacg cacgccacca gcaccaccgc cgcggcgact ccgccgccga tccacagcca
      181 aggcagttgc gagccgcctt gccccggata gacggctggc ctcagagcgc ttcccgccgg
      241 cgcggccagc ggattgccgg caggcgcggc gctagatagc gcgtcgagcg gattggccgc
      301 gggcgcgggc ggcgccggcg gcgctatgcg gacaacgaac ggcttttggc aactcgggca
      361 tggcacctgc ttgccgacga cgcttgccgc ggccccgaat cgggcgccgc aatgcggaca
      421 tacggccacg ccaatctggg acatacattc ctcccctgat gacgccttcc gacctgcgcg
      481 cgggttagaa aagccttgcc gctcgccgcg aatagtctat cagatgcccc ggcattgcaa
      541 catttccacc ccaacggaac ggcgaatcgc ctggaccggc ggcggcggcg agcttttcct
      601 ctatggctca ccggggcaat ctcgcggtgt gcaggcgaga atgcagattg gccaccatag
      661 tcgaaaggca cttttcaggg aaacaccgcc tcggacaacg tccgttcgcg ggtataagat
      721 gcaaggactc tccgctcgtc cagaatccca aagatgcgaa cgtttattgt cattttggtc
      781 gtgttggccg tcctggcggt cggcggcgtc gtggggtacc ggccggcggt cgatgcctgg
      841 cggaagcaca gcatgccgaa gtggcggacg gcggaggtga ccgaggggga gatcgtcgag
      901 acggtgaatg ccaccggcac cgtgaagccg gtgctgcaag tttcggtggg ctctttcgtc
      961 tcggggccga tcctggagct gtattgcgag ttcaatcagg aggtgaagaa ggatgaattg
     1021 ctggccaaga tcgatccgcg gatttaccag gcggccgtcg atggcgggcg agcgaatttg
     1081 ctgacgcggc aggccgacgt gaaacgcgtt gaagcccagc ttgcccaggc ccgccgcgat
     1141 gaaaatcggg cccaggagct gcgccaggag gattcggctt tcatcgccca ggccgaaatc
     1201 gaccgcgtga cgtacggccg catggcgcta gaggcccagc tcgacctggc caaagccgcc
     1261 gtcgagcaag cgcgctttca gctcaagcgg tcggaagcgg acctggacta ctgtgaaatt
     1321 cgctcacccg tcgatggcat tgtcatcaac cgcaagatcg acccgggcca gacgttggcg
     1381 gcccagtttc agacgcccga gctgttcatc gtcgcccccg acatgcgaaa gaagatgcac
     1441 gtccacgctt cggtcgatga ggcggacatc ggactgatta gtacggccca aaaaaaatcg
     1501 ttgccggtga gcttcacggt cgatgcctat ccggacgagt tttcgggcac catcgaggag
     1561 attcggctga gttcgacgac aacgcagaac gtggtgacgt atcccgtggt ggttgccgcc
     1621 ggaaatcccg acctgaagct gctgccgggg atgacggcca acatttcctt tgaggtcgat
     1681 cgctgcgagg aagtactgaa gattccgaat tcggcgctgc ggtttttccc cacgccgcag
     1741 cacgtccgga aggaggaccg gccgatcctg gaaggcaagg tcgccgcgga cgaagagcag
     1801 gaacaagtgg ccagcgagac gttgtctgcc cgcgaccgcg ccgaagcccg ccgggaccgc
     1861 aaccggcatc acgtgtgggt cgtcgatggg tacgagctgc gggcggtcga agtgatcacg
     1921 ggcctttcgg acagcaagta cacgcaactc gtctcgggcg acctgaaagc cggcgacaag
     1981 ctggtcaccg gcattcagcc ggcgatgaat ggttggggtg gatagtcagt tgtcagtggt
     2041 cagttgtcag tgggagtcga aattagccaa ctgacaactg acaagaatca gacatgggct
     2101 tcctgttgac attgcgaatc gccttccgcg ccctgcgcaa aaataagatg cgcgccgggc
     2161 tgacggtgtt gggcgtggtg attgggattg cagcagtgac gacgatggtc tcgctgggac
     2221 agagcgccag ccagctcgtg caagaccagc ttgcgggaat cggcaccaac gtgatggtcg
     2281 tctttcctgg caaccaggaa cgcggcggcg tgcggcaggg gctggtgacc acgctgaccg
     2341 cagccgattc ggacgcgatc gccgaagatt gcccgtcgat cctggcctcg tcgccgatcg
     2401 tcggcgcgca ggggcagatc atttacggca actcgaatta tcgcccgcga gacgtgttgg
     2461 gcgtcggcaa ggactttccc atcgtccgca gttggaccct caagcgtggc ggcttcttca
     2521 cggagcgcga aatcgactcg tacgaccagg tctgcgtgat cggacacaca ctggtccgca
     2581 agctgttcca gacgagcgat cccatcgacc ggaccatccg catcgatcgc gtgccgttcc
     2641 gcgttgtcgg cgtgctcgac gccaagggag ccaacatggt cggcgaggat caggacgaca
     2701 tcatcgtgat gccctacacg accgtgcaga aacggctcca cgggtcggaa ttcgacaacg
     2761 tcaacgcaat tatctgctcg gcccgctcga tggaagcgat gatggaggcc aaaagcgaaa
     2821 tcaatcagct cctggccgag cggcaccgca ttcctcccgg cgagccgttc gatttcacgg
     2881 tcgcgacgac gattgaaatg gcgaacatgc tgggaatcgt gctggggacg ctgacgatga
     2941 tgatctcttc catcgccgga atttcgctgg tcgtcggcgg cgtggggatc atgaacatta
     3001 tgctggtcag cgtcaccgag cggacccgcg aaattggcat ccgcatggcg gtcggggcga
     3061 caagttggga catcctgcgg cagtttctgg tcgaagcgat cctgctgtca ttcatcggcg
     3121 gggcgattgg ttttgccttg ggcgtggcgg cttcggtggg ggtgaccatg ctgatcaact
     3181 cgctcacgac cgggacaaaa tggccgatcg tcatctcgct gccggcggcg atgctggcga
     3241 ttgtgtttgc cgcggcggtg ggggtattct tcggcttcta tccggcacgg cgggcgagct
     3301 tgctcgatcc gatcgaggcg ctgcgatacg aatgacgcga cagcgcccgt cgcggacgga
     3361 cagtttgtcg cgacgtctgc cgcttgtctg tagccgcgct tgccacaacg agagattccc
//
LOCUS       DASUGB010000843         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1759864, whole genome shotgun sequence.
ACCESSION   DASUGB010000843 DASUGB010000000
VERSION     DASUGB010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759864"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFV87_16020"
     CDS             <1..365
                     /locus_tag="VFV87_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105326.1"
                     /translation="IAMRAKMLLEDIDGGQPLSPTYPYPVQRWQLGPDLQWFFLGGEV
                     VVDFAIRIKAEQTGRPTWVAGYSNDVMAYIPSRRVLTEGGYEGGGAMVYYGLPTIWSE
                     RVEEEIIDEVRKQVAASK"
     gene            complement(451..1545)
                     /gene="pheA"
                     /locus_tag="VFV87_16025"
     CDS             complement(451..1545)
                     /gene="pheA"
                     /locus_tag="VFV87_16025"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5105327.1"
                     /translation="MSKKKSSGGSANSVRRELQRLDRDLLKGLSDRARAVQKLAKIRQ
                     GDGEPLYDLTEEQQALTELLDQNKGPLSGDAVRNVYREILGTARSLVKPIRVAYLGPK
                     YSYSHLAAIDRFGDGPDLTPVATIKAVFESIHFRQTDFGIVPIENSTDGRIVDTLDMF
                     ARLPTRITGEVQLAIHHHLLGKCSRGEVTEVYSKPQALSQCREWLAKNLSGAKQIEMT
                     STAIAAQIAADKPGAAAIASLEAGMHYGLAAIDSHIEDNKNNVTRFAVIGGEMPHRTG
                     RDKTSLMFEIAHKPGSLADAMLVFKRGRLNLTWIESFPMPSTKNEYLFFVELEGHQAD
                     SRVKKAVAALQRKTARLEVLGSYPRAATAE"
     gene            2046..4001
                     /gene="dnaK"
                     /locus_tag="VFV87_16030"
     CDS             2046..4001
                     /gene="dnaK"
                     /locus_tag="VFV87_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5105328.1"
                     /translation="MAAGEKIIGIDLGTTNSVVSVMEGSEPKVIANAEGNRLTPSVVA
                     FTEKGETLVGEPAKRQAVTNPTRTVYSIKRFMGRRHAEVETEEKMVPYKVTGGAQDYV
                     KVKVGDKEQTPQEISAKILRKLKEAAESYLGHKVNKAVITVPAYFNDAQRQATKDAGQ
                     IAGLEVARIINEPTAAAMAYGLDKKKDEKIVVFDLGGGTFDVSVLQVTPAEGEGDKQV
                     FEVISTNGDTHLGGDDFDEVLINYVAGEFQKENAMDLRKDPMALQRLQEACEKAKKEL
                     SSLPSTDINLPFITIVGGVPKHLTMTISRSKFEELADPLIERCRKPVMQALKDAKLQP
                     GQIDEVVLVGGSTRVPKVVALVKEIFGKEPHKGVNPDEVVAVGAAIQGSVLAGERKDV
                     LLLDVTPLTLGIETEGGVLTPLVEKNTTIPTEKKQVFSTAADNQSGVTVRVFQGERKM
                     ASDNRLLGEFDLSGIPPAPRGMPKIEVKFDLDANGILNVSAKDLGTGKENTVKIQQSS
                     GLNKDEIEKMRRDAESHADEDKQKFELATARNQADNMCYQLEKMMKEHADKLTDADKG
                     PLEQAIATTREKAKGTDVAAIKSAIEQLEAASHAFSKVLYEKGAAAGAAGTSNDIDTG
                     PAAGAGSHTQQAKGDDDVIEGEFEVKK"
     gene            4184..>5842
                     /locus_tag="VFV87_16035"
     CDS             4184..>5842
                     /locus_tag="VFV87_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5105329.1"
                     /translation="MAFRMDKLTVKSQEAVQAAQALAAEQGHSEIDPLHLLAAMLQDG
                     EGIARPVLEKIGVNIAQLGKQIASELNRKPSMSGGSQPQPNRLFMTVLEEAVQQATVM
                     KDEFVSVEHLLLALTRVDSPAKNLLQVNGVREKDILEALKSVRGSARVTDQNPEGQYQ
                     ALEKYGIDLVQKAQAGKLDPVIGRDNEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLALRIVDGDVPESLKNRRVVALDMGRLIAGAKYRGEFEERLKAVIREVQESAGRVIL
                     FIDELHTVVGAGASEGAADAANLLKPALARGELRCIGATTLDEYRKHVEKDAALERRF
                     QPVFVGEPSVEDTIAILRGLKPRYEAHHGVKIKDSALVAAAKLSHRYIADRFLPDKAI
                     DLVDEAASKLSMELQSVPTEIDEVQRRVRQLELASRQLAEETDEHAVQRRDELDAEIA
                     EQKRKLASLKEQWEAEKLGVGDVAKLRKDLDRAELEYKQLENTIKEKQSAGIPPTEQD
                     YQQLLKLYNQRQTLEKQLAASDEKADAESEDGAAKQKPAGKRLLS"
BASE COUNT         1227 a         1812 c         1870 g          933 t
ORIGIN      
        1 acatcgccat gcgggccaag atgctgctgg aggatatcga cggcggccag ccgctttcgc
       61 cgacgtaccc ctacccggtc cagcgctggc agcttggccc ggacctgcaa tggttctttc
      121 tcggcggaga agtggtggtc gatttcgcga ttcgtatcaa ggccgaacag acgggccggc
      181 cgacctgggt cgccggctat tccaacgacg tgatggccta cattccctcc cgccgcgtgc
      241 tgaccgaggg cggctacgaa gggggcggcg caatggtcta ttacggcttg ccgacgatct
      301 ggtcagagcg cgtcgaagag gagattatcg acgaagtgcg gaaacaggtc gcggcgtcca
      361 agtaagttcc gacggccgcg gcggaaccgg atacgccaaa caggtcaact ggcacagcag
      421 gtcccgccca ggcggtcggg acctacaatg ctactcagcc gtggccgcgc ggggatagct
      481 ccccagcact tccagccggg cggtctttcg ctgcagggcg gcaacggcct ttttgacgcg
      541 cgagtcggcc tggtgccctt ccagctcgac gaagaacaga tactcgttct tggtgctggg
      601 catcgggaat gattcgatcc aagtcaggtt cagccgcccg cgcttgaaga ccagcatcgc
      661 atcggccagc gagccgggct tgtgggcgat ctcgaacatc agcgaagtct tgtcgcggcc
      721 cgtgcggtgc ggcatctcgc cgccgatgac cgcgaaccgc gtgacgttgt tcttgttgtc
      781 ctcaatatgg ctgtcgattg ccgccaggcc atagtgcatg ccggcttcca ggctggcgat
      841 ggcggcggcc ccgggcttgt cggcggcaat ctgcgcggca atcgccgtgc tggtcatctc
      901 gatctgcttg gcccccgaaa ggttcttcgc cagccactcg cggcactgcg aaagggcctg
      961 cggcttgctg tagacctcgg tcacctcgcc gcgggagcac ttgccgagca ggtgatgatg
     1021 aatcgcgagt tgcacctcgc cggtgatccg cgtcggcagg cgggcgaaca tgtcgagcgt
     1081 atcgacaatc cgcccgtcgg tgctgttctc gatcggcacg atgccaaagt cggtctggcg
     1141 gaaatggatc gactcgaaga cggctttgat cgtggcgacc ggcgtcaggt ccgggccgtc
     1201 gccaaagcgg tcgatcgccg ccaggtggct atagctgtat ttcgggccga ggtaggccac
     1261 gcggatcggc ttgacgagcg aacgggccgt gcccaggatc tcgcgataga cgttgcggac
     1321 cgcgtcgccg ctgagcgggc ctttgttctg gtccagaagt tcggtcaggg cctgctgttc
     1381 ttcggtcagg tcgtagagcg gctcgccgtc cccctggcgg atcttggcca gcttctgcac
     1441 cgcccgggcc cggtcggaca ggcctttgag caggtcgcgg tccaggcgct gcaattccct
     1501 gcgaaccgaa tttgcggagc cgccggagga tttctttttg ctcatcgtgc gagccaagta
     1561 ggtgaagcag aaagcgaccg atcaacaaga gtccatccaa actttaggtc gctccatgcc
     1621 tgaccaactc cgccggtggc ggggttgcgg tccacttgta gcgatggcaa ttcgcagtgt
     1681 caaccaagcg gcttttgcca cttttgccga attgtggtca gggatggcca gtttggcagc
     1741 ccctagacgg ggtgcattgg gtttggcttt acccccactt tgtcatattg acagtttttg
     1801 ctgccgagta cgctcgctcg ttcgctactt cctcggctcc gctttctaca aggttctatg
     1861 tggcaatggc ttacgaaaat cagtgcgctg ccgcgacatt ctggcacggg ctttgccatc
     1921 tgtccgccta tcgcacgccg cgggtccgat ttggcccacc tcgccaaccc ggcgcgcgac
     1981 caaagaagca accaattcaa aacgagatac agctcggcag acaccggcag aaaaaggaga
     2041 ccacgatggc cgctggagaa aaaatcattg gcatcgacct gggcacgacc aactcggtgg
     2101 tgtccgtgat ggaagggagc gaacccaaag tcatcgcgaa cgcggagggc aaccgcctga
     2161 cgcccagcgt cgtcgcgttc accgagaagg gcgagacgct cgtcggcgag cccgccaagc
     2221 ggcaagccgt caccaatccc acgcgcacgg tctattcgat caagcggttc atgggccgcc
     2281 gccacgcgga ggtcgagacc gaagagaaga tggtgccgta caaggtcacc ggcggcgccc
     2341 aggactacgt gaaggtcaag gttggcgaca aagagcagac cccgcaggaa atctcggcca
     2401 agatccttcg caagctcaag gaagcggccg agtcctacct gggccacaag gtcaacaagg
     2461 cggtcatcac ggtcccggcg tatttcaacg atgcccagcg gcaagcgacg aaggacgccg
     2521 gccagattgc cggcctcgaa gtcgcccgca tcatcaacga gccgaccgcc gcggcgatgg
     2581 cctatggcct ggataagaag aaggacgaga agatcgtcgt gttcgatctg ggcggcggca
     2641 cattcgacgt ctccgtgctg caagtcacgc cggcggaagg cgagggcgac aagcaggtct
     2701 tcgaagtcat cagcaccaat ggcgatacgc acctgggcgg cgacgacttc gatgaagtcc
     2761 tgatcaacta tgtcgccggc gagttccaga aagagaacgc catggacctg cgcaaggatc
     2821 cgatggcgct ccagcgcctg caggaagcct gcgaaaaggc caagaaggag ctcagctcgc
     2881 tcccctcgac cgatatcaac ttgcccttca tcacgatcgt gggtggcgtg cctaagcacc
     2941 tgacgatgac gatcagccgc agcaaattcg aagagctggc cgatccgctc atcgagcgct
     3001 gccgtaagcc ggtaatgcag gccctcaagg atgccaagct ccagccgggc cagatcgacg
     3061 aagtcgtcct ggtcggcggc agcacgcggg tgccgaaggt cgtcgcgctc gtcaaggaaa
     3121 tcttcggcaa agagccccac aagggggtca acccggatga agtcgtggcg gtcggcgcgg
     3181 ccattcaagg ctccgtcctc gccggcgagc gcaaggacgt gctgctcctg gacgtgacgc
     3241 cgctcacgct cggcatcgag accgaaggag gtgtgctgac gccgctggtc gagaagaaca
     3301 ccaccattcc gaccgagaaa aagcaggttt tcagcaccgc tgccgataac cagtcgggcg
     3361 tcaccgtccg cgtctttcaa ggcgaacgga agatggcgtc cgacaaccgg ctgctgggcg
     3421 agttcgacct ctcgggcatt ccgccggcgc cgcgcggcat gccgaagatc gaagtcaagt
     3481 tcgacctcga cgccaacggc atcctcaatg tcagtgccaa ggatctcggc acgggcaaag
     3541 agaacacggt caagattcag cagtcgtcgg gcctgaacaa ggacgagatc gagaagatgc
     3601 gccgcgatgc cgagtcgcac gccgacgagg acaagcagaa gttcgagctg gcgaccgccc
     3661 gcaaccaggc ggacaacatg tgctaccagc tcgaaaagat gatgaaggag cacgccgaca
     3721 agctcaccga cgcggacaag ggcccgctgg agcaggcgat cgccacgacg cgcgagaagg
     3781 ccaagggcac cgatgtcgcc gcgatcaaaa gcgccatcga acagctcgaa gccgcctcgc
     3841 acgccttcag caaggtgctc tacgaaaagg gcgccgccgc gggcgctgcc ggcacctcca
     3901 acgacatcga caccggcccc gccgccggcg ccggctcgca cacgcagcaa gccaaaggcg
     3961 atgacgacgt gatcgaaggg gagttcgagg tgaagaagta gcccgtgggg taggcgtctc
     4021 gcctgcccta cattaacccg aagcgtcagc gagggagtat cgtagcccgt agcgcaagcg
     4081 agggcattgt gggttgaacc acgagcgcgc atcgacttga cgacaacgcc aacgcttcgt
     4141 gcgcctccgc gtacggagcg ttgttcattg aaggattgag aacatggcct tccgcatgga
     4201 taaactcacc gtcaaatccc aggaagcggt tcaggctgcg caggccttgg ccgcggagca
     4261 ggggcattcg gaaatcgatc cgctgcacct cttggcggcg atgctgcagg acggagaggg
     4321 catcgcccgg ccggtgctgg agaagatcgg cgtcaatatc gcccagctcg gcaaacagat
     4381 cgcgtccgaa ctcaaccgca agcccagcat gagcggcgga tcgcagccgc agccgaaccg
     4441 gctgttcatg accgttctgg aagaagcggt gcaacaagcg actgtgatga aggacgagtt
     4501 cgtctcggtc gagcacttgt tgctcgcgct gacgagggtc gattcgccgg ctaagaatct
     4561 gctccaagtc aacggcgtcc gcgagaagga cattcttgaa gcgctgaaaa gcgtgagagg
     4621 cagcgcccgc gtcaccgacc agaacccaga gggccagtat caggccctgg aaaaatacgg
     4681 catcgacctg gtgcagaagg cgcaggcggg caagctcgac ccggtgatcg gccgcgacaa
     4741 cgaaatccgc cgcgtgatcc aggtcctctc gcgccgcaca aagaacaacc cggtcctcat
     4801 cggcgagccg ggcgtcggca agacggcgat cgtcgagggg ctcgccctgc ggatcgtcga
     4861 tggcgatgtg cccgaaagcc tgaagaaccg ccgcgtcgtc gctctcgaca tgggccgcct
     4921 catcgccggg gcgaaatacc gtggcgaatt cgaggaacgc ctcaaagcgg tcattcgcga
     4981 ggtgcaggaa tcggctggcc gcgtgatcct gttcatcgac gagctgcaca cggtcgtggg
     5041 cgcgggcgct tcggaagggg cggctgatgc ggcgaacctg ctcaagccgg ccttggcccg
     5101 cggcgaattg cggtgcatcg gcgcgacgac gctcgatgaa tatcgcaagc acgtcgaaaa
     5161 agacgccgcc ctggagcgcc gcttccagcc ggtctttgtc ggcgagccga gcgtggagga
     5221 cacgatcgca atccttcgcg gtctcaagcc gcgttacgaa gcccatcacg gcgtgaagat
     5281 caaggacagc gccctggtcg cggcggccaa gctctcgcac cgctacatcg ccgaccgctt
     5341 tctgcccgac aaggcgatcg acctggtcga tgaagcggcc agcaagctct caatggagct
     5401 gcagagcgtg ccgaccgaga tcgacgaagt gcagcgccgc gtccgccagc ttgaattggc
     5461 ctcgcgccag ctcgccgagg aaaccgacga gcacgcggtc cagcgccgcg atgagctgga
     5521 tgcggagatc gccgagcaga agcgcaaact cgccagtctc aaggagcagt gggaagcgga
     5581 aaaactcggc gtcggcgacg tggccaagct gcgcaaggac ctggaccgcg ccgagctgga
     5641 atacaagcag ctcgaaaaca cgatcaagga gaaacaatcc gccgggatcc cgccgaccga
     5701 gcaggattac caacagctcc tcaagctcta caaccagcgg cagaccctcg aaaagcagct
     5761 cgccgcgtcc gacgagaagg ccgacgcgga atctgaagat ggcgcagcca aacaaaagcc
     5821 ggcgggcaaa cgcctgctca gc
//
LOCUS       DASUGB010000844         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1759954, whole genome shotgun sequence.
ACCESSION   DASUGB010000844 DASUGB010000000
VERSION     DASUGB010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759954"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFV87_16040"
     CDS             complement(<1..413)
                     /locus_tag="VFV87_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105330.1"
                     /translation="MLSPCRTSVAAASLLIATAVFAFSATDSKAQGRSGACEESNLAV
                     LASPLAPWKGAPLRVVFTSEKPLDGELSLVAPDGSVAAKSRKRFGGPPYFWFAEVNAP
                     AAGKWRASLASPDCGNVAREISVRSDQPPRPQTAAG"
     gene            complement(902..1048)
                     /locus_tag="VFV87_16045"
     CDS             complement(902..1048)
                     /locus_tag="VFV87_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105331.1"
                     /translation="MTKIELEQIDDSILNFDISDEALESAGDNAVAANYTLGACTGLS
                     VCPG"
     gene            complement(1242..>3248)
                     /locus_tag="VFV87_16050"
     CDS             complement(1242..>3248)
                     /locus_tag="VFV87_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5105332.1"
                     /translation="SEVRPFSEKQIELVGTFADQAVIAIENARLLSELRESLQQQTAT
                     ADVLKVISSSPGNLEPVFEGILESATQICQAGFGTLNLYEDNAFRSVALHNPPPQYRA
                     RLGEVIHPHPDSGLAHVARTKQIAHIEDVRTQKPYLKGSPAVVGLADLAGARTLLIVP
                     MLKEGNLLGAVSIYRQEIRPFTDKQIELVQNFAAQAVIAIENARLLSELRESLQQQTA
                     TSDVLKVISRSTFDLPTVLQTLVESAATLCDADKTNITRQRDGAFYRAEAHGFSREFM
                     DYVRNIPIAAERGSAFGRALLEGRAVHIPDVTADADYTSVDLQRLGDYRTVLAVPMLR
                     EGTAIGVLTLTRSEVRPFTAKQIELASTFADQAAIAIENVRLFDEVQGRTRELARSLE
                     DLRTTQDRLVQTQKLASLGQLTAGIAHEIKNPLNFVNNFSAVSGELIDDLQGALKGIP
                     LDGKVRAEIDELTTTLRGNLDKVVQHGKRADSIVKNMLLHSREGSGEHRIVDINAIVE
                     ESLNLAYHGARAEKQGFNVTLERSFDARAGEADLFPQEITRVLLNLISNGFYATTKRN
                     AEGSGNGYEPTLMAATKDLGDSVEIRIRDNGTGMPPEVKEKIFNPFFTTKPAGEGTGL
                     GLSLSHDIVVKQHGGSIEVDTRPGEFTEFRVILPRSGATLAKTE"
BASE COUNT          610 a          958 c          995 g          685 t
ORIGIN      
        1 cccgccgcag tttgcggccg cggcggctga tcggagcgca cggagatttc gcgggcaaca
       61 ttgccgcagt ccggcgaagc gaggctcgcc cgccattttc cggcggcggg tgcgttcact
      121 tcggcgaacc agaaatacgg aggcccaccg aaccgcttgc gcgatttggc ggcgacgctt
      181 ccatcgggcg ccaccagcga caattcgccg tcgagcggct tctccgaggt gaagacgacg
      241 cgaaggggcg cgcccttcca aggcgcgagc ggcgaagcca ggaccgcgag atttgactcc
      301 tcgcaagcgc cggagcggcc ctgcgccttc gaatctgtgg ctgagaacgc gaaaaccgcg
      361 gttgcgatca gcagcgaggc cgccgcaacg gaagttcggc acggtgacag caagcgaccc
      421 ccctgtcctg aatcgtccct gggccaagta aactatagtg ggcagacctg gccaaccaat
      481 cctccgaatt tcggtgaaat tagggagatt ccgccgctgc ccgccgggaa cgggaacttt
      541 tttgccagcc gggtacttgt ttgggcagat gggagacatg ccatgaccaa actggacatg
      601 ggtcctgata ccgctcaagc caccgacgac attgcgtggc ggcatttttg gctggccagt
      661 gtggtggtgg aaaccgacgg gcggccgcct gtgcgtgccc cccgccgtga attgcggccc
      721 cgaaaagaat cgctaagccg gcgcctgcaa aggcttcgtc atatgcgggc gatactttac
      781 gcaccgcggc actgagatcg tcgctttatc tggttttgta cccgcgccgg tcatggcgcg
      841 ggtacatttc gtatcagagc cgatcgcgca gatctcgcag cggatcggcg atggacgtcg
      901 atcagcccgg gcagaccgac aggccggtgc aggcaccgag cgtatagttg gccgcgacgg
      961 cgttatcgcc cgcggactcg agtgcctcgt cggaaatgtc gaagttcaga atgctgtcgt
     1021 cgatctgttc gagctcaatc ttggtcatgg cctatatatc cttttcctgg ctcccctgtg
     1081 gagctgtaca caatgtagga ttagagcgtc aaattgcatg tgacggaagt cacactactt
     1141 gcgtttttcc ggcgcggccg gacgtagacg tcgtgttttc gcgggaaaca gaacaacgga
     1201 tacgtcagtc gttaaccttg cttgcggtta tttcctcgag atcattctgt cttcgcaagc
     1261 gttgcgcccg agcgcggaag aatgacccgg aattcggtaa actcgccggg cctggtgtca
     1321 acctcgatgg agcccccgtg ctgcttcacg acgatgtcgt gactgaggga aagcccgagc
     1381 ccagtccctt caccggcagg cttggtcgtg aaaaacggat tgaatatctt ttccttcacc
     1441 tcgggcggca tgccggtgcc gttgtcgcgg atgcgaatct cgacgctgtc gcccagatct
     1501 tttgtcgcag ccatgagtgt cggctcgtag ccattgccgc tcccttcggc attgcgtttc
     1561 gttgtcgcgt aaaagccgtt ggagatcagg ttgagcaaca cccgcgtgat ctcctgcggg
     1621 aacagatcgg cctcgcctgc gcgcgcatcg aatgaccgtt cgagcgtgac attaaaccct
     1681 tgcttctcgg cccgcgcccc gtgataggca agattgaggc tttcctccac gatggcattg
     1741 atatcgacga tccggtgctc gccggaacct tcgcgcgagt gcagcagcat gttcttgacg
     1801 atcgaatccg cgcgcttgcc gtgctggacc accttgtcga gattgccccg cagggtcgtc
     1861 gtcagctcgt cgatttcggc gcgcactttg ccgtccaacg gaatgccttt cagcgcgccc
     1921 tgcaaatcgt cgatcagttc gccggaaaca gccgagaaat tgttcacgaa attgagcggg
     1981 ttcttgatct cgtgcgcgat ccccgcggtg agctggccaa gcgaggcgag tttttgcgtc
     2041 tgcaccaggc ggtcctgcgt ggtgcgcagg tcttcaagcg atctggctag ctcgcgcgtg
     2101 cgcccctgca cttcgtcgaa caggcgcacg ttctcgattg caatcgcggc ctggtcggcg
     2161 aaggtcgaag ccagctcgat ttgcttcgca gtaaatggcc ggacttccga acgcgtcaaa
     2221 gtcagcacgc cgattgcagt gccttcgcgt aacatcggga cggcgaggac cgttcgataa
     2281 tctcccagcc gttgtaaatc gaccgaggtg tagtccgcgt cagccgttac gtcgggtata
     2341 tgcaccgcgc ggccttcaag cagcgcccgg ccgaacgcgg agccgcgttc ggccgcaatg
     2401 ggaatattcc ggacgtagtc catgaattcc cgggaaaaac cgtgtgcttc ggcacgataa
     2461 aaagccccgt ctctttggcg cgtgatattg gttttgtcgg catcgcacag cgtcgccgcc
     2521 gactcaacga gtgtctgcag cacggttggc aaatcgaacg tcgagcggct gatgaccttg
     2581 aggacgtcgg aggtcgcggt ctgctgctgc agcgactcac gcagttcgct gagcagacgc
     2641 gcgttctcga tcgcgatgac cgcctgcgcg gcgaagtttt ggaccagctc gatctgcttg
     2701 tcggtgaagg ggcgtatctc ctggcgatag atagagactg cgccgagcaa atttccttcc
     2761 ttcaacatag gaacaatcag cagcgtgcga gcaccggcaa gatcggcaag tccgacgacg
     2821 gctggactgc ccttgaggta gggtttctgg gttctgacgt cctcgatgtg cgcaatctgt
     2881 ttggttcggg ccacatgggc caggccgctg tccgggtgcg gatgaatgac ctcgcctagc
     2941 cgtgctctgt attgaggcgg cggattgtga agtgcaacac ttcggaaggc gttgtcctcg
     3001 tacagattca aggtgccaaa cccagcctgg caaatttgag tggcgctctc aagaatccct
     3061 tcgaaaaccg gctccaggtt acctggtgaa gagctgatga ccttgagcac gtcggcggtg
     3121 gcggtctgct gctgcagtga ttcacgcaac tcgctcagca gtcgagcgtt ctcgatcgcg
     3181 atcacggcct ggtccgcgaa agtacccaca agttcaatct gtttttcgct gaacgggcgc
     3241 acttcgct
//
LOCUS       DASUGB010000845         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1342457, whole genome shotgun sequence.
ACCESSION   DASUGB010000845 DASUGB010000000
VERSION     DASUGB010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342457"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1832
                     /locus_tag="VFV87_16055"
     CDS             297..1832
                     /locus_tag="VFV87_16055"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5105333.1"
                     /translation="MLIRRFLPEEGVKLGIWTAVIWLACQSPAQAQSPALPGQAEGGG
                     LRVGVAEVDITPPDGFPMAGYYYERLAEGAIDPLKAKAIVWRGRQAEACLVVCDLIGI
                     AADLSREVRRRASEKTGIPPSHIVVSATHSHTAPDYMKELYLNLGGQRQEPSRAAYIE
                     KLISGPVEAIVKARAAAEPAFLESGSATQDQAVSFNRRFVMRDGSVRTWMNLQHPDVV
                     RPAGPIDSQIGLLSIKRMTGETVGVLSNFALHLDTVGGSKWSADYPYFIEQKLKQTRG
                     AQVVSIFATGCCGDINHADPRRPQRNKVDFIGGEIGATIDRQLGQLQRLSEPGLTVKS
                     RVVQLPLQEASAEEVAHSLQVLQAAARKEKVEFFQHVTAYKKVILDQLRHREPHANAP
                     EHITWGLSRSLAGVGDSLPVDVTVIALGPEVALVFLPGEVFVELGLAIKQGSPFATTL
                     VVELSNCVETIYIPTRAAYAGGGYEVTNSSLQAGGGELLVESALGLLREAATAQLPAA
                     AKQ"
     gene            complement(1993..>2217)
                     /locus_tag="VFV87_16060"
     CDS             complement(1993..>2217)
                     /locus_tag="VFV87_16060"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105334.1"
                     /translation="IWLAGGGIKGGRTYGQTDEFGHKAAVDVVTPSDFQATVLHLLGL
                     DHARLVYHANGRAQTLTNGQAARVVREILA"
BASE COUNT          392 a          685 c          734 g          406 t
ORIGIN      
        1 gcgatcgaag tcgtcagtgc gctgcaagaa acgcgtcgct ttgggcggta gtgaggcacg
       61 ttgaccactc gggacgaggc gcgcatgcgg tgcgtcattg cgacctggct tcctcgtcgg
      121 acgccgctgg gttctgagtg ccgtctggag ggccgaagcg gaggaaatgc cccggaccac
      181 atgttaaagt gacgcgtcga gcgtgctcgg gactgtccgc cgccctgcaa tcagccttga
      241 acggctgacc cggctcgcga ttccttgtgc ggcgaattca attgcgaggc tgtcccatgc
      301 tgatccgacg atttctacct gaagagggcg tcaagctcgg catttggacg gctgtgattt
      361 ggctggcctg ccagtcgccg gcgcaagctc agtcgccggc gctccccggc caagcagagg
      421 gtggcgggct gcgcgtcggc gttgccgaag tggacatcac gccgccggac ggattcccga
      481 tggcaggcta ctattacgag cggttggccg agggggcaat cgatccactc aaggccaaag
      541 cgattgtctg gcgaggccgg caggccgaag cgtgcctggt ggtgtgcgac ttgatcggga
      601 ttgccgcgga cttgtcccgt gaagttcgcc gccgagcgtc cgagaaaaca ggcattcccc
      661 cgtcccacat cgttgtgtcg gcgacccatt cgcacacggc gccggactac atgaaggagc
      721 tatacctgaa tctcggcggc cagcggcagg agccgtcgcg agcggcctac atcgaaaaat
      781 tgattagcgg ccccgtggaa gccatcgtca aagctcgtgc ggcggcagag ccggcgtttt
      841 tggaatccgg ttcggcaacg caggaccaag cggtgtcgtt caaccgccgt ttcgtcatgc
      901 gggacggcag cgtgcggacg tggatgaatc tccagcatcc cgacgtcgtc cggccggcgg
      961 gcccgatcga ttctcagatc gggctgttgt cgatcaagcg catgacgggc gaaacggtgg
     1021 gcgtcctcag caattttgcc ctgcacctgg atacggtggg cggatcgaag tggagcgcgg
     1081 actatccgta tttcatcgaa cagaagctga agcaaacgag gggcgctcag gtcgtctcca
     1141 tcttcgccac cggctgctgc ggagacatca accacgccga tccgcgccgc ccccagcgga
     1201 acaaggttga cttcatcggc ggcgagatcg gggcgacgat cgacaggcaa ctcgggcagt
     1261 tacagcggct ttcagaaccc ggcctcacgg tgaaatctcg tgtcgtccag ttgccgctgc
     1321 aagaggcatc ggccgaggag gtggcacatt cgctgcaggt tctccaggct gcggcacgga
     1381 aggagaaggt cgagttcttc cagcatgtca ccgcttacaa gaaggtgatc ttggaccagc
     1441 tgcgccaccg cgagcctcat gcgaacgcgc cggagcacat aacctggggc ctcagccgtt
     1501 cgctggcggg agtaggcgat tcattgcccg tcgatgtcac cgtgatcgcc cttggtcccg
     1561 aagttgcgct cgtcttcctg cccggggagg tctttgtcga actggggctg gcaatcaagc
     1621 aaggctcgcc ctttgctaca acgctcgtgg tcgagctttc caactgcgtg gaaacaattt
     1681 acattcccac ccgcgcggcc tacgccggcg gcgggtacga ggtgacgaat tcttcgctgc
     1741 aggccggcgg cggggagctg ctcgtggaat ccgcgctggg cctgttgcgc gaagcggcca
     1801 cggcgcaatt gccagctgcc gccaagcagt aggcccgcgc gccggttcgc gctccggtcc
     1861 cccacgccgg tcccgccaag gaggatcgag ttcgcggatc tcgcgaagag gcgctgcgcc
     1921 ccaaccaaac tacctcgcca tagatcggcg gcacctcggg cgcactgagt gagggctttt
     1981 aacgcctggc aatcaggcga ggatttccct gaccacccgt gcggcttgac cgttcgtcag
     2041 cgtttgagcg cggccgttgg catgatacac cagccgcgcg tggtcgagcc ccagtaagtg
     2101 caggaccgtc gcctggaagt cgctgggcgt gacgacatcg acggctgcct tgtggccgaa
     2161 ttcgtcggtc tggccgtagg tccggccccc tttgatgccg ccgccggcga gccagat
//
LOCUS       DASUGB010000846         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_787620, whole genome shotgun sequence.
ACCESSION   DASUGB010000846 DASUGB010000000
VERSION     DASUGB010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787620"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VFV87_16065"
     CDS             complement(<1..1209)
                     /locus_tag="VFV87_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5105335.1"
                     /translation="MTTHRFSRRTMLRGLGVTMALPWLESLPVWGDELPAKHTASQAP
                     VRLAVLFAGNGFHSKEWWAKGAGRDMELGQVLAPLADFREKLLFIRGLYNAEALKGNI
                     HSSQTGNLLSGAPLASGGDIRSGTSIDQLLAQSYGRSTKVPSLVLGCEKSNPSVHKNY
                     SMLYSSHISWTSPTTPTPLELYPALAFDRLFKDEAARGDKSVLDAVLADAADLRRQIS
                     TPDQRKLDEYLDSVRDVEQRIAAAGQRGELQGWRPTLDKPNIPRPPDGIPQDIAEHMR
                     LMCDILVLGFQTDTTRLTTLKLNNDHSSLRFPHLGVDYMIHHLLSHSDTADWLKVNQF
                     FTGQLAYIARKLDAIQEGERTLLDNSMLLFCSSMMTGGHDATQLPVVLVGRGGGKIQS
                     GRVLDYLGQEN"
     gene            complement(1365..>2218)
                     /locus_tag="VFV87_16070"
     CDS             complement(1365..>2218)
                     /locus_tag="VFV87_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HEX5105336.1"
                     /translation="HIHDFDHLDEKSDRHFPTFVSLRGAMYEESIQFFTDLFQHDRSV
                     LGVLDADYTFLNEQLAAHYNIPGVSGDQWRRVDGMKQYARGGILAQASTLAKQSGASR
                     TSPILRGNWISEVLLGERLPRPPKGVPPLPDDESASEGLSVRQLVERHTSDEKCSVCH
                     RRIDPLGFALEGFDAIGRRRDKDVGDRPVETSVKTIDGAEFTGLDGLRSYLAGTRREA
                     FLRQFCKKLLGYSLGRAVQLSDEPLLAKMQADLAASDYRVQTAIEDIVHSRQFREIRS
                     RDNAYDE"
BASE COUNT          360 a          690 c          756 g          412 t
ORIGIN      
        1 gttctcctgg cccaggtaat cgaggacgcg gccggactgg atttttccgc cgccgcggcc
       61 cacgagcacc accggaagct gcgtggcgtc gtgaccgccg gtcatcatgc tcgaacagaa
      121 gagcagcatc gagttgtcca ggagcgtccg ctcgccctcc tggattgcgt cgagcttgcg
      181 ggcgatgtag gccagctggc cggtgaagaa ctggttcacc ttcagccagt cggccgtgtc
      241 ggaatgcgac agcaggtggt ggatcatgta atcgaccccc aggtgcggga aacgcaacga
      301 gctgtggtcg ttgttcagct tgagcgtcgt gagccgcgtc gtgtcggtct ggaaacccag
      361 caccagaatg tcgcacatca gccgcatgtg ctcggcgata tcctgcggaa tgccgtcggg
      421 cggtcgcgga atgttcggct tatcgagcgt cggccgccag ccttgcagct cgccgcgctg
      481 gccggcggcg gcgatccgct gctccacgtc gcggaccgaa tcgagatact cgtcgagctt
      541 ccgctggtcg ggcgtgctga tctgccggcg gaggtcggcg gcgtcggcca gcaccgcgtc
      601 gagcacgctc ttgtcgccgc gggccgcttc gtccttgaac agccggtcga aggccagggc
      661 tggatacagc tccagcggcg tgggggtggt ggggctggtc cacgagatgt gcgagctata
      721 cagcatcgag taattcttgt ggaccgacgg gttcgacttc tcgcagccga gcaccaggct
      781 gggcaccttg gtggagcggc cgtagctttg ggccaggagc tggtcgatgc tcgtgcccga
      841 gcggatgtcg ccgccggagg ccagcggggc cccggagagg agattgccgg tctgcgagct
      901 gtggatgttt cccttgaggg cttcggcgtt gtagaggccg cggatgaaga gcagcttctc
      961 gcgaaaatcg gccagcgggg ccagaacctg gcccaactcc atgtcgcggc cggccccctt
     1021 ggcccaccac tccttgctgt gaaagccgtt gccggcgaat agcaccgcca ggcgcaccgg
     1081 ggcctggctg gcggtatgct tcgcgggcag ttcatcgccc caaaccggca gcgactcgag
     1141 ccacggcagg gccatcgtca cgcccaggcc gcggagcatc gtgcggcgcg aaaagcggtg
     1201 cgttgtcata gtgggccttc ggaatgccaa tgaggaacag gtctcgtcac atcacttcct
     1261 gggaggaagc cacggttgaa acaccgatgg aacacggatt tgggtaaagc tgtctgcttc
     1321 atccgtgtcg atccgtgttt catccgtggc taaattctgc ggtctcattc gtcgtacgcg
     1381 ttgtcgcggc tgcggatttc acggaactgg cggctgtgga cgatgtcctc gatggccgtt
     1441 tgcacccggt aatcgctggc cgccaggtcg gcctgcattt tcgccaagag cggctcgtcg
     1501 gacaattgca ccgcccggcc cagcgaatag cccagcagct ttttgcagaa ctggcggagg
     1561 aacgcctcac ggcgcgtgcc ggccaggtag ctgcgcaggc cgtcgagccc cgtaaactcc
     1621 gccccgtcga tcgtcttgac gcttgtctcg accggccggt cgcccacgtc cttgtcgcgc
     1681 cggcggccga tggcgtcgaa tccctccagg gcaaagccga gcgggtcgat ccggcgatgg
     1741 cagaccgagc atttctcgtc gctggtgtgc cgctcgacaa gctgccgcac gctcagccct
     1801 tcgctggccg actcgtcgtc aggcagcggc ggcacgccct tgggcggacg cggtagccgc
     1861 tcgcccagca ggacctcgct gatccaattg ccgcgaagga tggggctggt gcgcgaggcc
     1921 cccgattgct tggcgagcgt ggaggcctgg gcgagtatgc cgccccgggc gtattgcttc
     1981 atgccatcga cccgccgcca ctgatcgccg ctgacgccgg gaatgttgta gtgggcagcc
     2041 agctgctcat taaggaacgt gtagtcggcg tcgaggacgc ccagcaccga gcggtcgtgc
     2101 tggaacaagt cggtgaagaa ctggatcgat tcctcgtaca tcgctccgcg caacgagacg
     2161 aatgtgggga agtgccggtc gctcttctca tccaggtggt cgaagtcgtg gatatgca
//
LOCUS       DASUGB010000847         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_927421, whole genome shotgun sequence.
ACCESSION   DASUGB010000847 DASUGB010000000
VERSION     DASUGB010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927421"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV87_16075"
     CDS             complement(<1..183)
                     /locus_tag="VFV87_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105337.1"
                     /translation="MSDDADSPASDLTMDEILLALLIRLEAIGDEHEELFDSEVRERM
                     GNAVFHGFVKPMYDFQL"
     gene            complement(180..1607)
                     /locus_tag="VFV87_16080"
     CDS             complement(180..1607)
                     /locus_tag="VFV87_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105338.1"
                     /translation="MYRIPSFNSRREFLAQAGGGLGAIALASLLQGSEATTLTRSVSE
                     GTKPPHFEPKVKRVIWLFMHGGPSHVDLFDPKPDLIKYAGQPLPESFGMVMTRRKVAE
                     NPLLGPVKPLKPRGDSGVEVSDFLPHIAELADDLCVIRSLHGDSVNHPQSVYQMNTGS
                     ILMGHPSVGSWVAYGLGSENRDLPAFVVLPDPGGGVKGGPPAWGSGFLPATYQGVTMR
                     PGDQPILHLRPQPGISAAQQRATLELTLELSRRHQAARGDDDELAARIKAYELAFRMQ
                     AAAPELVDLKDESAETLALYGMDEKETREFGQRCLLARRMVERGVRFVQVYSGDTNGW
                     DAHNDVLDNHTKLCRSTDKAVAGLLRDLKRRGLWNDTLVVWGGEFGRMPMSEQGKGRD
                     HNPWGYCAWLAGGGVKAGFAYGATDPVGLRAAENKVHIRDFHATLLHLLGLDPLALSY
                     YHNGLDERLVGPDDKEVRVVKEVLA"
     gene            complement(1670..>2010)
                     /locus_tag="VFV87_16085"
     CDS             complement(1670..>2010)
                     /locus_tag="VFV87_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX5105339.1"
                     /translation="PAIDFHKRYNEGDETIPQMYPDPEAARQDTAILHVHPLNWPRHQ
                     FFCCDPEDYRWHESADRLRMKLYSLGIPHTCDLETTGGGHGFEYYNRMAERAIGFLAE
                     KLDEERRRIV"
BASE COUNT          320 a          642 c          675 g          373 t
ORIGIN      
        1 caattgaaag tcgtacatcg gcttgacgaa tccatgaaac acagcgttgc ccattcgctc
       61 gcgaacctcg gaatcgaata attcctcatg ctcgtcgccg atcgcctcga ggcgaatcag
      121 gagcgccaag agaatctcgt ccatggtcaa gtcgctggcc ggagagtcgg cgtcgtcgct
      181 cacgccaaga cttccttcac cacccttacc tccttgtcat ccggaccgac gaggcgttcg
      241 tcgaggccgt tgtggtagta gctgagggcg agggggtcga ggccgaggag gtgcaggagc
      301 gtggcgtgga agtcgcggat gtggactttg ttctccgcgg cgcggaggcc gacggggtcc
      361 gtcgcgccgt aggcgaagcc ggctttgacg ccgccgccgg ccagccacgc gcagtagccc
      421 caggggttgt ggtcgcggcc tttgccctgc tcgctcatcg gcatgcggcc gaattcgccc
      481 ccccagacga ccagcgtgtc gttccagagg ccgcggcgct tcaggtcgcg cagcaggccc
      541 gcaaccgctt tgtcggtcga gcggcaaagc ttcgtgtggt tgtcgagcac gtcgttgtgg
      601 gcgtcccagc cgttggtgtc gcccgaatag acctggacga accgcactcc ccgctcgacc
      661 atccgccggg ccaagaggca ccgctggccg aactctcggg tttccttttc atccatgccg
      721 tagagggcga gcgtctcggc tgattcgtcc ttgagatcga ccagctccgg cgcggcggct
      781 tgcatccgga aggcaagctc gtaggccttg atgcgggcgg ccaattcgtc gtcatcgccg
      841 cgcgccgcct ggtggcggcg gctaagctct aaagtcagct ccagtgtggc ccgctgctgg
      901 gcggcgctga tgccgggctg cggacgcaaa tgcaggatcg gctgatcgcc gggccgcatc
      961 gtgacgccct gatacgtcgc gggcaagaag ccgctgcccc aggcgggcgg cccccccttc
     1021 acgccgccgc ccggatcggg caggacgacg aacgccggca ggtcgcggtt ctcgctcccc
     1081 aggccatagg cgacccagct tccgacgctg gggtggccca tcaggatgct gccggtgttc
     1141 atctgataga ccgactgcgg gtgattcacg ctgtcgccgt gcaggctgcg gatcacgcac
     1201 aggtcgtcgg ccagttccgc gatgtgcggc aggaagtcgc tgacctccac gcccgaatcg
     1261 ccccgcggct tgagcggctt gaccggcccc agcagcgggt tctcggccac tttgcggcgg
     1321 gtcatcacca tgccgaagct ctcgggcaac ggctggccgg cgtacttgat cagatcgggc
     1381 ttcggatcga acagatcaac gtggctcggg ccgccgtgca tgaagagcca gatgacgcgc
     1441 ttgaccttgg gctcgaaatg gggtggtttg gttccctcgc ttacgcttcg ggttagtgtg
     1501 gtcgcttcgc tcccctgcag cagcgacgcc agggcaatcg ccccgaggcc accgccggct
     1561 tgggcgagga actcgcggcg ggagttgaag ctgggaattc ggtacatgag ccgccggccc
     1621 cgagatacgc ataaacggcg actctgattg tagccgaacg cggcccggac tacacaatcc
     1681 gccggcgttc ttcgtccagc ttttcggcca gaaagccgat cgcccgctcg gccatgcggt
     1741 tgtagtattc gaagccgtgg ccgccaccgg tggtttccag gtcgcaggtg tgcgggatgc
     1801 ccagcgaata gagcttcatc cgcaggcggt cagcactctc gtgccagcgg taatcctccg
     1861 ggtcgcagca gaagaactgg tgccgcggcc aattgagcgg atggacgtgc aggatggccg
     1921 tgtcctgccg ggctgcttcc gggtcgggat acatctgggg aatcgtttcg tccccttcgt
     1981 tgtagcgctt gtggaagtca atggctgggg
//
LOCUS       DASUGB010000848         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_233411, whole genome shotgun sequence.
ACCESSION   DASUGB010000848 DASUGB010000000
VERSION     DASUGB010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233411"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFV87_16090"
     CDS             <1..579
                     /locus_tag="VFV87_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5105340.1"
                     /translation="NSDIDRQRRIDFLKRAIDAAEALGSDCVSLWSGVREGDASDEAT
                     LERLAESLRTVLRHATHSGVTLGFEPEPGMFIDTMKSFSRLLQWIDAPHLQLTLDVGH
                     LWCQGELPIADYIARWGPRIVNVHLEDMRAGVHEHLMFGEGQMHFPPILEALAKTGYQ
                     GGLHVELSRHSHEAPIAAKKAYDFLKPLLPAL"
     gene            637..2019
                     /locus_tag="VFV87_16095"
     CDS             637..2019
                     /locus_tag="VFV87_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX5105341.1"
                     /translation="MYNHVILLSIPGLRSQDLAAMPNLSRLTAGGDRAMLTPSFPCVT
                     WPVQANMLTGKLPREHGVVANGFYWRETHEVEMWTAWNDKIQQPQIWDLLHTNPKRKR
                     ESEERLTSAVWFPMLSKGCGADYICMPKPVHNPDGSESLWCYTKPVEMYGTLRDTFGH
                     FPLQHFWGPLANIKSTAWIADSAAWAMREFRPNFFYIYLPHLDYAAQKTGPDSPQALA
                     AVKELDDVLGKLFAAGNAAYTPTRNVSEGVKRSASEGPLWLIASEYVITPVDHVTYPN
                     RTLREAGLLTVKNDGTGELIDFANTPAWALVDHQFSHVFVKDRDAATIQRAAELFRGQ
                     TGIAEVLAGNERGKYALDHERSGDVILISTQSSWQAYYYWLDDANAPKFARTVDIHNK
                     PGYDPVELHFDFATKSIPLDATLTKGSHGAPATTDAQRGVLLSSKAIGATGQLRDTDL
                     CKLAIDQFRD"
BASE COUNT          406 a          745 c          578 g          325 t
ORIGIN      
        1 aattccgata ttgaccggca acggcggatc gacttcctga agcgggcgat cgacgccgcg
       61 gaagccttgg gtagcgactg cgtttcgctc tggtcgggcg tgcgcgaggg cgatgcgtcc
      121 gacgaagcca cgctggagcg cctcgccgaa tcgctccgca ccgtgctgcg ccacgcgacg
      181 cacagcggcg tcacgctcgg ctttgagccc gagcccggca tgttcatcga cacgatgaag
      241 tccttcagcc gcctgctgca gtggatcgac gccccgcacc tgcagctcac gctcgacgtg
      301 gggcatctct ggtgccaggg cgagctgccg atcgccgact atatcgcccg ctgggggccg
      361 cggatcgtca acgtccacct cgaagacatg cgggccggtg tccacgagca cctgatgttc
      421 ggcgaagggc agatgcactt cccgccgatc ctcgaagccc tcgccaagac cggctaccag
      481 ggcggcctcc acgtcgagct cagccgccac agccacgaag cgccgatagc ggcgaagaag
      541 gcatacgatt tcctgaaacc gctgctgccc gcgctgtagg gaacgccctc cgtggcgttc
      601 cgcgaactgt caaccgactc ccatctttta ccttccatgt ataaccacgt catcctcctc
      661 tccatccccg gcctccgcag ccaagacctc gcggcgatgc cgaatctctc gcggttgacg
      721 gcggggggcg accgggcgat gctcacgccg agctttccct gcgtgacctg gcccgtgcag
      781 gcgaacatgc tcacgggcaa gctgccgcgc gagcacggcg tcgtcgccaa cggcttctac
      841 tggcgcgaga cgcacgaagt cgaaatgtgg accgcctgga acgacaagat ccagcagccg
      901 caaatctggg acctcttgca cactaacccg aagcgtaagc gagaaagcga ggaacgtctc
      961 acctccgccg tctggttccc catgctcagc aaaggctgcg gggccgacta catctgcatg
     1021 cccaagccgg tccacaaccc cgacggcagc gagtcgctgt ggtgttacac caagccggtc
     1081 gagatgtacg gcacgctgcg agacaccttc ggccatttcc cgctgcagca cttctggggc
     1141 ccgctggcga acatcaaatc gaccgcctgg atcgccgatt ccgccgcctg ggccatgcgg
     1201 gaattccggc ccaatttctt ctacatctat cttccccacc tggactacgc cgcccaaaaa
     1261 accggcccgg acagtccgca agcacttgcc gccgtcaaag agctcgacga cgtcctcggc
     1321 aagctcttcg ccgccggcaa cgcggcctac acaccaaccc gcaacgtaag cgagggagtg
     1381 aagcgcagcg caagcgaggg gccgctctgg ttgatcgcca gcgaatacgt catcaccccc
     1441 gtcgatcacg tcacctaccc caaccgcacg ctccgcgaag ccggcctgct caccgtgaag
     1501 aacgacggca ccggcgagct gatcgacttc gccaacacgc ccgcctgggc cctggtcgat
     1561 caccagttct cgcacgtctt cgtcaaagac cgcgacgccg cgacgatcca gcgcgccgcc
     1621 gaactcttcc gtggccagac cggcatcgcc gaagtcctcg ccggcaacga gcgcggcaag
     1681 tacgctctcg accacgagcg gtccggcgat gtcatcctga tctccacgca gagcagttgg
     1741 caggcctatt actactggct ggacgacgcc aacgccccca agttcgcccg caccgtcgat
     1801 atccacaaca aacccggcta cgaccccgtc gagctgcact tcgacttcgc caccaaatcg
     1861 atccccctcg atgccacgct caccaaaggc tcccacgggg caccggcaac caccgacgcg
     1921 cagcgcggag tgctgctcag ttcgaaggct atcggagcca ctggacaact acgcgacacc
     1981 gacttgtgca agctcgcaat cgaccagttt agggattgaa cctgtggaac tgccgacgat
     2041 tcctggcttc aaag
//
LOCUS       DASUGB010000849         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1204863, whole genome shotgun sequence.
ACCESSION   DASUGB010000849 DASUGB010000000
VERSION     DASUGB010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204863"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFV87_16100"
     CDS             complement(<1..710)
                     /locus_tag="VFV87_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5105342.1"
                     /translation="MPQSVLSRYIDPDVLGHVADRHLEPAGLVVGNLAGAHKSPLSGF
                     AVEFAGHREYVPGDDPKHVDWRVYFTREKYFVKQYELETNFVCHLLLDVSASMRYGEA
                     REQKLLYAAQMATMLGYSVIRQSDKISLLTFDDHVRGFVTPSNSMSQIVRMTEHLDQI
                     DPVEKTAMAACLNDIAARLGRREIVMLFSDFFTDLDELEPVLQRLRYNKHEVVLLQVM
                     HHDELAFELDGMVKFIGL"
     gene            complement(744..986)
                     /locus_tag="VFV87_16105"
     CDS             complement(744..986)
                     /locus_tag="VFV87_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105343.1"
                     /translation="MILYEEGTYIQFGKTPAGLCGTGLKRVAGPSAPTVSFADRDMYL
                     LNDIQQIRSIDFQLSQVSATRIAAFKQEYPNCYVAP"
     gene            complement(1145..2173)
                     /locus_tag="VFV87_16110"
     CDS             complement(1145..2173)
                     /locus_tag="VFV87_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5105344.1"
                     /translation="MTDKTHPDDVAAVQKCERAYNRLRDELSKVIVGQNEVIEQVLVA
                     MFARGHALLEGVPGLAKTLLISSLAKSLHLTFKRIQFTPDLMPSDVTGTEVIQEDPET
                     HQRAYRFLPGPVFANMLLADEINRTPPKTQAAMLEAMQERQVSAGGKMHALPAPFFVL
                     ATQNPLEQEGTYPLPEAQLDRFLLYIKVDYPSAAEEWEVARRVTTGQQGEIEAVLSGE
                     EILEMQKLVTRVPVSDQVLGYAWALVRATRPGQKEAPEFVNKWVNWGAGPRGVLTLVT
                     CAKARAILYGRYHATVADVQAIAKPALRHRIAGNYAAQANGLNSEKLIDMLFESVPAD
                     RKYERPAA"
     gene            complement(2253..>2648)
                     /locus_tag="VFV87_16115"
     CDS             complement(2253..>2648)
                     /locus_tag="VFV87_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105345.1"
                     /translation="QQIAGPQANAAAQQAMAAAQGQAGQGQGQSQQPMPGQASSTARS
                     GGAASQGDTANNQKPPPGDLETAATPEADSRGQAADQDANAARQQFEGEPWFAKLPPG
                     LRAAIQSKSRGKAPRGYEERLRRYFESVD"
BASE COUNT          436 a          832 c          847 g          533 t
ORIGIN      
        1 tccaggccga tgaatttgac catcccgtcc agctcaaacg ccagctcgtc gtggtgcatc
       61 acctggagca gcacgacctc gtgcttgttg tatcgcagcc gctggagcac gggttccaac
      121 tcgtccaggt cggtgaagaa gtcggaaaag agcatcacga tctcgcggcg gcccaggcgg
      181 gcggcgatgt cgttcaggca cgccgccatc gccgttttct ccaccgggtc gatctggtcg
      241 aggtgctcgg tcatccgcac gatttggctc atcgagttcg agggcgtgac gaagccgcgg
      301 acatgatcgt cgaacgtcag gagcgagatt ttgtcgctct ggcgaatgac cgagtagccg
      361 agcatggtcg ccatctgggc cgcatagagc agcttttgct cgcgcgcttc gccgtagcgc
      421 atcgacgcgc tgacgtccag cagcaagtgg cagacgaagt tcgtctccag ctcgtactgc
      481 ttgacgaagt atttctcgcg cgtgaaatag acgcgccaat cgacgtgctt gggatcgtcg
      541 ccaggcacgt attcgcgatg cccggcaaac tcgacggcga acccggacag cggcgatttg
      601 tgtgccccgg ccaggttgcc gacgaccaaa ccggccggct ccaagtgccg atcggcaacg
      661 tggcccagca cgtcggggtc gatgtagcgg gagaggacgg attggggcat ggcgcatgcg
      721 tcgtcacgtg ggacgtgcaa ttcctaggga gcgacgtaac agttcggata ctcttgcttg
      781 aacgcggcga ttcgagtggc ggacacttgg ctcaattgaa aatcaattga ccggatctgc
      841 tggatgtcat tcagcaggta catgtcacgg tcggcaaagc tcacggtggg agcagacggt
      901 ccagcgaccc gctttagacc cgttccgcac agtccagcgg gagtcttgcc gaactgaatg
      961 taggtccctt cttcatagag aatcacacag gcgcctttgc gggccagaag ttcaacggcg
     1021 cggtcctccc ttgcggcagc tcgcacacga tttccgtacc agccagccgc gatcgcaatt
     1081 gcgaacgaaa caaggaatag cgttgctagc cgaaactgca ttggttgctt catttgacga
     1141 gccctcaagc tgctggtctt tcgtattttc tgtccgccgg caccgattca aacagcatgt
     1201 cgatcagctt ctcgctattc aggccgttgg cctgggcggc gtagttgccg gcaatgcggt
     1261 ggcggagggc cggcttggcg atggcctgta cgtcggcaac cgtggcgtga tagcggccgt
     1321 agaggatggc ccgggccttg gcgcaagtga ccagcgtcag cacgccgcgc gggccggccc
     1381 cccagttgac ccacttgttg acgaattcag gcgcctcttt ttgcccgggg cgcgtcgccc
     1441 gcaccagggc ccaggcatag ccgagcacct ggtcgctcac cggcacgcgg gtcaccagct
     1501 tttgcatctc cagaatttcc tcgccggaca gcaccgcctc aatttctccc tgctggccgg
     1561 tggtcacgcg gcgggccact tcccattcct cggcggctga agggtaatca accttgatat
     1621 agaggagaaa gcgatcaagt tgggcctcgg gcagcgggta cgtcccttcc tgctcgagcg
     1681 ggttctgcgt cgccaggacg aaaaacggcg cgggcagcgc gtgcatcttc ccccccgcgc
     1741 tcacctgccg ctcttgcatc gcttccagca tggccgcctg cgttttcggc ggcgtgcggt
     1801 tgatctcgtc ggccagcagc atgttcgcga acaccggccc ggggaggaag cgatacgccc
     1861 gctgatgcgt ctcggggtct tcctgaataa cctctgtccc cgtcacgtcg ctgggcatca
     1921 gatcgggcgt aaactggatc cgcttgaacg tgaggtgcag gctcttggcg agcgagctga
     1981 tgagcagcgt cttggccagg ccgggtacgc cttcgagcaa ggcatggccg cgggcgaaca
     2041 tggcgacgag cacctgctcg atcacctcgt tttgcccgac gatcactttc gagagctcgt
     2101 cgcgcagccg gttataggcc cgctcgcact tctgcacggc ggccacgtcg tcgggatggg
     2161 ttttgtcggt catgttctct cgctccaatt agccgggacg taacggtttc cggatgccgg
     2221 agctgtcagc tcccggctgg ttggttcatt aactagtcca cgctctcgaa gtaccgccgc
     2281 agccgctcct cgtacccgcg gggggctttg ccgcgcgatt tcgattgaat tgcggcccgc
     2341 agccccggcg gcagcttcgc aaaccaaggc tccccctcga attgctgccg ggccgcattg
     2401 gcgtcctggt cggcggcctg gccgcggctg tcggcctcgg gcgtcgcagc ggtctctaag
     2461 tcgccgggcg gaggcttctg gttattggcc gtgtctccct ggctcgccgc gccgcccgag
     2521 cgggcagtgg aacttgcctg gccaggcatc ggctgctgcg attggccttg gccctggccg
     2581 gcctggccct gagcggcggc catcgcttgc tgggccgccg cgttggcctg cgggccggcg
     2641 atttgctg
//
LOCUS       DASUGB010000850         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1204903, whole genome shotgun sequence.
ACCESSION   DASUGB010000850 DASUGB010000000
VERSION     DASUGB010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204903"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFV87_16120"
     CDS             complement(<1..256)
                     /locus_tag="VFV87_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5105346.1"
                     /translation="MPCQPNQPSHVLLAVNPQAGARSRREMVEHLELELRRCGLTPEI
                     VADAAQLPGRAGELHAAGELRAVVAAGGDGTFRLVAENTPP"
     gene            809..1291
                     /locus_tag="VFV87_16125"
     CDS             809..1291
                     /locus_tag="VFV87_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105347.1"
                     /translation="MSFDDQLRQAIERGQRRGEHRAREAAVRAMTEEECRRLHSQLRL
                     SLSEHIEACVKRLPNYFPGFQYETMYGDRGWGGACFRDDLRLVRGTRSTDYSRLEVTV
                     RPYSAAHVLDLAAKGTVHNREVFNRNYFEPLDEVDASKFLQLIDAWVLEYAEMYAAKQ
                     "
     gene            complement(1336..3561)
                     /locus_tag="VFV87_16130"
     CDS             complement(1336..3561)
                     /locus_tag="VFV87_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5105348.1"
                     /translation="MTSDTLTSSSSRPLALAMRADLVAQRQQWQGREYWTIKDPLTLR
                     YYRFEEEEFAILSMLDGQTSAEDICERFEERFAPQRIRGSQLQQLLAMLHRSNLLVST
                     APGQGEQLHRRSQEQKNKQRLAALASFLALRFRGVDPDRFLAWLVRRAGWLFSLPAAI
                     FAALLVFSAVLLVAAEFDTFRARLPAFEAFFAVQNWFWLAAVLCVTKILHELGHGLAC
                     KRFGGECHELGVMLLVFTPCLYCNVSDSWMVPSRWRRAAIGAAGMYIELILASLATFV
                     WWFTEPGLVNHLALNVMFVCSVSTLVFNANPLMRFDGYYILADLLEIPNLRQKAQAVI
                     QRKLGAWLLGLRERPDPLLPTRHQWLFAAYSVASAVYMWLVSLSIFWFLYRVLEPYGL
                     KIVGQMLGLAMIFTLIVLPFVRLIRFLVQPARAETVNKSRAAISLGSIAAASAVVLLV
                     PLPYYVSCGFEIQPREASSVYVDVPGELKAVYSTNGTVAAGQTLAELEDVEARLTEEQ
                     LVAQGDKLAARIEGIRQRALTDDQALLELSQTEEARAAIEKQLNRLRQDLAKLTIRAP
                     AAGTIVPPPARPANEGDRTRLAAWSGRPLEVRNVGAYLEASTLLCRIAQPGQLEAILA
                     IEQEELDFVAAGQRVELLLAQLPGEKLAGTIDRIADENMQAAPARLAARSGGHLATRP
                     DALGIERPLSVVYQASVPLDDPSGQIIVGATGIAKIHAGYQPLALRLWRACCRTLRFE
                     L"
     gene            complement(3558..>3819)
                     /locus_tag="VFV87_16135"
     CDS             complement(3558..>3819)
                     /locus_tag="VFV87_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5105349.1"
                     /translation="IDRLRVEGFVSATDVAPGELAGRPVTVEVQLSRERSDRFRGQVV
                     FISPLVQAGNKYRVRAEVANRTENGQPVLRPGMSATLTIELP"
BASE COUNT          714 a         1285 c         1184 g          636 t
ORIGIN      
        1 cgggcggcgt gttttccgcg accagccgaa acgtgccgtc gcctcctgcg gctacgacag
       61 cccgcaattc gccggcggcg tgaagttccc cggcacgtcc tggcagttgt gctgcgtctg
      121 cgacgatctc cggcgtgagc ccgcagcggc ggagttcaag ctccagatgc tccaccattt
      181 cacggcggct gcgagcgccg gcctgcggat tgacggccag caggacatgc gacggctgat
      241 tcggctggca gggcatcgct gttctcacag ttttcccagt ttcaccgccg cccgcccgct
      301 tcgccaaggc cggatgacaa ccctaaccac ggcctcagat cgcgcgtgaa aggcaggtgc
      361 gcaagatcga ccacgattgc tcaatcccac accgcgtcaa ctcgcccgtt gttgcgaaac
      421 gaaacgcccg ccaaaccgca gccgcgaact gagcccatcg aaatcgtaca cggcacaatt
      481 tgacgcgtca caacctgttg tcgcacaaga cgttacagaa actgtgcaat cgtgcgtcaa
      541 cttttcttga tattggcgcg ctgcctgcta tggggaggtc atcacgccgc ggcaaaacgc
      601 ggcgcattcc cttcggctgg cccgtggggt ctctgacgac aatcgcggac gtggttgact
      661 tgcccctggc cactttcgca gcccaaaacg tttttggcgc gtcagagacc cccgtttttc
      721 ttttcttgag ccgccagatg gctgttgtcc agcctgcaag gacaagtggt acgtgctaaa
      781 acaaggattc ttccttgttt ccacggacat gagcttcgac gatcaacttc ggcaggccat
      841 cgagcgcggg cagcggcgcg gggagcaccg cgcccgcgag gcggctgtca gggcgatgac
      901 cgaggaggag tgccgccggc tgcactcgca gcttcggctg tcgctctcgg agcatatcga
      961 ggcgtgcgtc aaacgactgc cgaattactt ccccggcttc cagtacgaaa cgatgtacgg
     1021 cgaccgcggc tggggcgggg cctgctttcg cgacgacctg cggctggtcc gcgggacgcg
     1081 gagcacggac tacagccggc tggaggtcac cgtgcggccg tattcggcgg cccacgtgct
     1141 cgatctggcg gcgaagggga cggtccacaa ccgcgaggtc ttcaatcgca actacttcga
     1201 gccgctggac gaggtcgatg cgtccaaatt cctgcagctc atcgacgcgt gggtactgga
     1261 atacgcggag atgtacgcgg cgaagcagta gctccagatg atgggcctgc gctcgcttca
     1321 ctcgctggtc ccaccctaca gctcaaaccg cagcgtccgg cagcaggcac gccataggcg
     1381 cagtgcgagc ggctgatagc cggcatggat tttggcgatg cccgtcgccc cgacgataat
     1441 ctggcccgat gggtcatcca gcggcacgct ggcctgatag acgaccgaga gcggacgctc
     1501 gatgcccagc gcatcgggcc gggtggccag gtggccgccg ctgcgtgctg cgaggcgcgc
     1561 cggcgcggcc tgcatgttct cgtccgcgat gcggtcgatc gttcccgcga gcttctctcc
     1621 gggcagttgc gccaggagca attcgacgcg ctgaccggca gcgacgaaat ccaattcctc
     1681 ctgttcgatc gccagaatcg cttctagttg gcccggctgg gcaatgcggc acaggagcgt
     1741 gctggcttcc aggtacgcgc cgacgttccg cacctccagc ggccggcccg accaggcggc
     1801 caatcgcgtc cggtcgccct cgttcgcggg acgcgccggc ggcggcacga tcgttcccgc
     1861 agccggagcg cggatcgtga gcttggcaag gtcctgccgc aggcggttaa gctgcttttc
     1921 gatcgcagcc cgggcttctt cggtctgcga aagctccaag agcgcctggt cgtccgtgag
     1981 agcccgctgc cggatgccct cgatccgcgc ggccagcttg tcgccttgcg ccacgagttg
     2041 ctcttcggtc agccgggctt ccacatcttc cagttccgcc agcgtctggc ccgccgcgac
     2101 ggtgccgttg gtcgaataca cggccttcaa ctcaccaggc acatcaacat agacgctgct
     2161 cgcctcccgc ggctgaatct cgaagccgca cgagacgtag tacggcagcg gcacgagaag
     2221 cacgaccgca cttgcagccg caatcgatcc taaactgatg gcagctcgcg acttattcac
     2281 cgtttcggcc ctcgcaggtt ggacgagaaa acgaatcagc cgcacgaacg gcagcacgat
     2341 cagcgtgaag atcatcgcca gcccgagcat ttgccccacg atcttcaggc cgtacggctc
     2401 gagcacgcga tacagaaacc agaagatcga cagcgaaacg agccacatat agacggccga
     2461 agcgacgcta taggcggcaa agagccactg atgccgcgtc ggcagcagcg gatcgggccg
     2521 ctcgcgcagg ccgagcagcc acgcaccgag cttccgctgg atcaccgcct gcgccttctg
     2581 ccgcagattg ggaatctcca agaggtcggc cagaatgtag tacccgtcga accgcatcag
     2641 tggattggca ttgaacacca gcgtgctcac cgagcagacg aacatcacgt tgagggccag
     2701 atggttgacg agccctggct cggtgaacca ccagacgaac gtggccagtg atgcgaggat
     2761 cagctcgata tacatgccgg ccgcgccgat cgccgcccgc cgccagcggc tgggcaccat
     2821 ccacgagtcg cttacgttgc aatacaggca gggcgtgaag accagcagca tcacccccag
     2881 ctcgtgacat tcgccgccga agcgcttgca cgccagcccg tgccccagct cgtgcaggat
     2941 tttcgtcacg cacagcacgg ctgccagcca gaaccagttc tgcacggcga agaacgcttc
     3001 aaatgccggc agccgggcgc gaaacgtgtc gaactccgcc gccaccagaa gcacagccga
     3061 aaacacgagc agagcggcaa aaatggccgc cggtagcgag aagagccagc cggcgcgccg
     3121 caccagccac gcgagaaacc ggtccggatc gacgccgcga aagcggagcg ccagaaagct
     3181 ggccagcgcc gccagccgct gcttgttttt ctgctcctgc gagcggcgat ggagctgctc
     3241 gccctggccg ggcgcggtcg atacgagcag attgctgcga tgcagcatcg ccaggagctg
     3301 ctgaagctgg ctgccgcgaa tccgctgcgg ggcgaaacgc tcctcgaacc gctcgcagat
     3361 gtcctcggcg ctcgtttgcc cgtcgagcat cgacaggatc gcgaactcct cctcttcgaa
     3421 gcgatagtaa cgcagcgtca gcgggtcctt gatcgtccaa tactcccgcc cctgccactg
     3481 ctgccgctgg gcgaccaggt ccgcgcgcat cgccagcgcc aaagggcggc tgctgctgga
     3541 ggtgagcgta tcggacgtca tggcagctca atcgtcagcg tcgccgacat tcccggccgc
     3601 agcaccggct ggccgttctc ggtgcgattg gccacttcgg cccgtacgcg gtacttgttg
     3661 ccggcctgca cgagcggact gatgaagacg acctgaccgc ggaaacggtc gctacgctcg
     3721 cgggaaagct gcacttccac cgtcaccggc cgcccagcca gttcaccggg agcgacgtcg
     3781 gtcgcgctga cgaacccttc tacccgcagg cggtcaatg
//
LOCUS       DASUGB010000851        11502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1343353, whole genome shotgun sequence.
ACCESSION   DASUGB010000851 DASUGB010000000
VERSION     DASUGB010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343353"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="VFV87_16140"
     CDS             <1..1942
                     /locus_tag="VFV87_16140"
                     /inference="COORDINATES: protein motif:HMM:NF039750.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105350.1"
                     /translation="LFLRRESPPRTPPRQRRVLLDAGIRTWGVPRVFVAAVGLALAAQ
                     SDPQLAVSTYSAAAGEAEPVDFRTKAGLKRHLAVLDHRLHPAESLAVLSKAATSGRGS
                     QGRARKGRESIAAGRLNHGESRPQLTPDPDECDLILVTTDDTLADRDFQRWLYEAGPR
                     TLYQAAVSRDGRFQLLLRSFRGTKPICQAKFDLDEVLKPRPTSPKLLDPSRDPEFPAI
                     FSGRMPLRLSCPVDVERSWLVHPGTVASYTRDGRLLLWDSPQHGARQIAHGLPEGSLM
                     WCSSAWEDDTLRLVLGKRSQRGLVSVTYDRRQGTCETKTLKLTGYHPQHVIGYQGHGY
                     VFYSNEFDILSLQTGELVANHALRLYPETSSLFFPSNAHSQRRNWVSIANFIQPVFTE
                     TDDIKLLALHHAFGHEGPLGITDKGEILDPATSRLTPINTSLLPNRRHFAPPYSLVGV
                     SRDGLRAMIRPVQVPNLAMLVEVPSGTARECLAFDPAALERPMFEIAKPRVVRSRFSA
                     IGVGRDRQLVLVSRRNHLWPILLDVGNRTIRFPKDPHTSLLLDKLEFEPIDSERGFEL
                     HVVEWPDGSRAWLDGRGMLHLDSSNPQIPQCSLILTDGPLAGWLSDGRVFGPPYWLQG
                     QQPTSAETVYREVLQPFVAQLG"
     gene            1939..4782
                     /locus_tag="VFV87_16145"
     CDS             1939..4782
                     /locus_tag="VFV87_16145"
                     /inference="COORDINATES: protein motif:HMM:NF039752.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105351.1"
                     /translation="MNVTLQLRFSETPLRSAAAWLIPGGEPSGWLTELARWNVPLAEA
                     GLCVIHAESGEAIGVVVVLPGNAKPTPTPLARPYGRIAGRLLLPVEAALDPPLADAEV
                     VELLPAGDATFVWHPAAGLLRFDAEAALSVAELLTPPPAGDRNWGSAQPGIATNQRLL
                     SIEPAQRPSIEEVLLAGRDDIGTQPMHGADLPRAPGEPVGGAIGRAIAGAGMAAAAGA
                     AGMIAAAAQAVSAGLSGLTGGEAGAAASAAPQRRAAASGGSQGPNWIERLAQWARQKQ
                     QAMRQNIDQMRHSQIERLLSALQKSPDLGLRFALPLTSLGAARGHAPPGARLIERNIN
                     FNLNRLGGGGPVDVWDLPDDYRRKLALRYRELANREISLGRHRRAAYILAELLGDLTS
                     AAATLAEGQHFREAAQLYETRLNQPLAAARCLQRGGLLTEAVALYERLSEWETVGELY
                     EQLDQPDRAAEAFRRAVTRRISAFDHLGAAKLLETKLAVPDEALTTLLGGWNGSSQAE
                     RCLDEAFDLLARHQKNDRARSLIDELSTRPAKPVVLTGGLARLATKFPDAGVRETAAS
                     SARRLVSAHLPTAAPYESESLLRSLGSLVPADRLLERDCRRYREQQIEKDRRRIEQAS
                     RRQPRSKLPTPIRTFRLPPGEWKAAVGAGEEFYAAGIHAESLLVVRGRFDGAVQLPMG
                     DVCKLTAEQAAAPVFLAADPRGLAFTYVHVPPDKPWRDLRFAATDKYQRAMQVSPIWG
                     GEKPLEGLIYSSGGSLLLLTLPEPGIAQVTPYDPQLRLRGVVSLTYSIDALDSDDDIH
                     RPLPFFARGDSLYLAIGSRLISKRGGQESSRLYSPIRSITGSPLHTRPRIVLACEDGG
                     YVQWGESARSPATRFAQDMHQPVVGLTRGGWLAASVRGELEVHSTEGGRLSFIGRTSA
                     PTEDPLAILPTGDTSRFALVTASGLVTVYEVPG"
     gene            complement(5023..6534)
                     /locus_tag="VFV87_16150"
     CDS             complement(5023..6534)
                     /locus_tag="VFV87_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5105352.1"
                     /translation="MARHNLTILLISSVLSLVCYRQALMSRYGATLSEALSIIDREYI
                     EPVDSRILFEGAMDGMMNKLDPYSGYTPPDEYNQFQETMDGEFGGIGIVVEVNQETGR
                     LTVLSPLVGTPAYKAGLRSGDTILAIDDHDTEGMSLPDSVKLMRGGVGSKVKVRIQHA
                     SEPEPVEYELARANIPIESVLGDARRADGTWVYHLVDRPRIGYVRIINFGERTGEELK
                     TALQSFREPGQQIDGLIIDLRGNAGGLLNAAQEVCDAFLDHGVIVTTRGRNGVERERR
                     EASLGTELPDDVPLVVLIDKYSASASEIVAACLQDHERAMVVGQRSWGKGTVQNVISL
                     EGGRSALRLTIGSYWRPSGHDIHKREGAKDTDDWGVRPDPGQEVNFTNQEFETVVLAR
                     RRRDVTSYEDLIAAKTSAPRAPAELPAPESPPLPSPPTPSPDELAAPSLDVANPPVPV
                     PDAESDDPAIRQAEQRAAANALKDPATIDAQLKQAIQRLEEEIRLRAGRPQSA"
     gene            6792..8141
                     /locus_tag="VFV87_16155"
     CDS             6792..8141
                     /locus_tag="VFV87_16155"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5105353.1"
                     /translation="MWITLAILATAALALSAVLSLHKWESRRFVRRGLTNRVQRDEAI
                     QAVLIVPCKGIDIGQVENLRALFEQRQIDYQLRLVVEDERDSAAELIAALQAEFPHVR
                     SELLIAGRAIESGQKVHNLLIATENLPADVNVLAFADSDARPGPDWLAALVSRLNELQ
                     IGARTGYRWMVPERMTWPNLLVYSINCSVASMYSRHRLNAVWGGAWAIRRELFEQVGI
                     REAWAGTLSDDLVVTRVLRASGLQIEFEPRCLTASPVDMTAREAVNFLRRQFLLVRRY
                     APLHWWLALAGSSLLQGGLWGAAVLTLGSAIAGQAWWLPGLMGLTLYVLAMFRGWLGQ
                     SAFRARLAGHESKLASPRRFDILLGPLAGVCGWLTMLSSLVGSSMTWRGIRYWISPGG
                     RVLLLGRVVEAGSLSGAIRMPGRSSGDKALEIQRRALERGIRLHDAATEVPRDRKAA"
     gene            8186..9181
                     /locus_tag="VFV87_16160"
     CDS             8186..9181
                     /locus_tag="VFV87_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5105354.1"
                     /translation="MALASLTPATRPVGATDAAPLLSQNLDHESQALDEFRRANADLF
                     GKSDLWLALHLAVVLTLLGLGTWAIVVVPLVWVKILLGPVNAFLWFCLGNVTVHHHHT
                     HHNAAKSAVFKRLLDGLHFLVVPDGGRGLKRYRRAHMNHHLHPLSDSDVDHRYAQKHY
                     QKMSKSVWTRIVYFLELTFIGGHVPGQKDEAYLNRVPIAKWNLAEYEKVKEREVRKAK
                     RNSLIAWGIFLVVLFVFPPLAWAWIYPLILVRNWNGFLGQFQHYDEKLLASGRSKHNR
                     TLTFRIPGWLNYLAGGEISGHFIHHIYPDMPYYHVESARRRLLRQPELVRLVVNH"
     gene            9280..9969
                     /locus_tag="VFV87_16165"
     CDS             9280..9969
                     /locus_tag="VFV87_16165"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5105355.1"
                     /translation="MIVRSPRRPSRIHSSETFADLLQRLGDVAPERIRLDPWPGKVTD
                     EDVAESKDRFGCLCELVDGVLVEKIMGVPESALATELSRMIKNFLDEHPLGMVCGPDG
                     PVRFQSRLIRMPDISFFSWDRIPGDRMEEDAMLGIVPDLVVEVISPGNRPGEMRVKLQ
                     EYFQAGVRLAWYIHFKQRKAQIYTSIDDVTDVSLNDCLDGGDVLPGFRVRLQTVYDRA
                     FPTRPKKKRGK"
     gene            complement(9975..>11502)
                     /locus_tag="VFV87_16170"
     CDS             complement(9975..>11502)
                     /locus_tag="VFV87_16170"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5105356.1"
                     /translation="VALAAALFTVLHQMNDPASLIRGDSRQLVVGIFLAAALLAALRL
                     KTGEILTSSGLLAGWLIARKMVHKTGILAPQPDSPWFDWLLPAADPRQGPVMLLVLAA
                     AVTVVLVLVWRHGEGKPQLPPSGIDADFKRIFPFSHTNGLAPLDVWIPLLIAARFRVG
                     FKYVPRLLAVLVVSAVNTLLSLPERLLLPLILRNKRVVDPIFVVGTHRSGTTHLHNLL
                     ALDPQFCTARNYQTMNGAGFLLSGWLVTPLLCLFMPWKRPMDGVRFSMFTPQEEEFGL
                     AGCSVMSPHWGMTFPRLWPHFDRYIFPDRLPAAERQVWKHQYLLLLRKHSLWTQKRLL
                     LKNPYNTARVGILREMFPGAKFVHICRHPHVVYRSNIHLAREGHVCNQLQDPDERNSY
                     EARFLDNYRAMEQAFADDAQRLPANAVARLRFEDLERDPIGGIEAIYEQLGIEFSADY
                     RRRLERYLAGLQGYEKNRHQPLADGARAQIAEKMGPFMRQHGYVDESATPASLERRAA
                     "
BASE COUNT         1975 a         3671 c         3768 g         2088 t
ORIGIN      
        1 gctgttcctc cgccgcgaat cgcccccgcg cacgccgccg cggcagcggc gcgtgctgct
       61 cgatgccggc atccgcacct ggggcgtgcc gcgggtcttc gtcgccgcag tcggcctggc
      121 tttggcggcg cagagcgatc cgcagcttgc cgtcagcacc tacagcgccg ccgccggcga
      181 ggcggagccg gtcgatttcc gcaccaaggc cggcctcaaa cggcacctcg ccgtgctcga
      241 ccaccggctg cacccggcgg aatcgctggc cgtgctttcc aaagccgcga ccagtggtcg
      301 cggttcccag gggcgggcga gaaagggtcg ggagtcaatt gcggcgggcc gcctgaatca
      361 tggagagtca agaccgcaat tgactcccga ccccgacgag tgcgacctga ttctcgtcac
      421 gaccgacgac acgctggccg atcgcgattt tcagcggtgg ctctacgaag ccggcccgcg
      481 cacgctctat caggccgccg tcagccgcga cgggcgcttt caactgctct tgcggagctt
      541 tcgcggcacc aagccgattt gccaagcaaa attcgatctg gacgaagtcc tgaagccgcg
      601 gccgaccagt cccaagctgc tcgaccccag ccgcgatccc gaattcccgg caatcttctc
      661 cggccgcatg ccgctgaggc tgtcgtgccc ggtcgatgtc gagcgttcgt ggctcgtgca
      721 tccgggaacc gtcgcttcct acacccgcga cggccggctg ctgctctggg atagcccgca
      781 gcatggggcc cggcagattg cgcacggcct gccggaaggt tcgctgatgt ggtgttcgtc
      841 cgcgtgggag gacgacacac tgcgcttggt actcggcaag cggagccagc gtggcctggt
      901 gagcgtgact tatgaccgcc ggcagggaac ctgcgagacc aagacgctca agctcaccgg
      961 atatcatccg cagcatgtca ttggctatca aggccacggg tacgtctttt attcgaacga
     1021 attcgatatt ctcagcttgc agacgggcga gcttgtggcc aatcacgctc tgcggctcta
     1081 tccagagacg agctccctgt tcttcccgag caatgcccat tcgcagcggc ggaattgggt
     1141 gtccatagcc aactttatcc agcccgtctt cacggagacg gacgacatca aactgcttgc
     1201 tctgcatcac gccttcgggc acgaaggtcc gcttggcatc accgacaagg gcgagattct
     1261 tgatcccgcg acaagcaggc tgacgccgat caacacctcg ctcttgccga accgccgcca
     1321 tttcgctcct ccgtattcgc tcgtcggcgt ttcgcgcgat ggtttgcggg cgatgatccg
     1381 accggtgcaa gtgccgaacc tcgcgatgct ggtggaggtc ccaagcggca cggcgcgcga
     1441 gtgccttgcg ttcgacccgg ctgctctcga gcggccgatg ttcgagatcg ccaaaccccg
     1501 cgtcgtccgc agccgcttct cggcgatcgg cgtcgggcgc gaccggcagc tcgtgctcgt
     1561 ctcgcgccgc aatcatttgt ggccgatcct tctggacgtg ggcaaccgga cgatccgctt
     1621 tcccaaagac ccgcacacca gcttgctgct cgacaagctg gagttcgagc cgatcgattc
     1681 cgaacgcggc tttgagctgc atgtcgttga atggccggac ggcagccggg cctggctcga
     1741 tggccgcggg atgctgcacc tggatagctc caatccgcag attccgcagt gttcgctgat
     1801 cctgacggat ggcccgctcg cgggctggct ctccgatggc cgcgtgtttg gcccgccgta
     1861 ctggctgcaa ggccaacagc cgacgtcggc cgaaacggtg tatcgcgaag tcctgcaacc
     1921 tttcgtcgcc cagcttggat gaacgtcacg ctgcaactcc gattttccga aacgccactg
     1981 cgcagcgccg cggcctggct gattcccggc ggcgagccga gcgggtggtt gacggaattg
     2041 gcgcgttgga acgtgcctct ggccgaggcc gggctctgcg tcattcacgc ggagagcggc
     2101 gaggcaatcg gcgtcgttgt cgtgcttccc ggcaacgcga agcccacgcc cacgccgctg
     2161 gcccgccctt atggccgcat cgccggccgc ttgctcttgc cggtcgaagc agccctcgat
     2221 ccgccgctcg ccgatgcgga agtggttgag ctgttgccgg cgggcgacgc gacgttcgtc
     2281 tggcatccgg cagcgggctt gctgcgattt gacgcggagg cggcactttc ggttgccgag
     2341 cttcttacgc cgccgcctgc cggcgaccgc aactggggct ccgcccagcc cggcatcgct
     2401 accaaccagc gcctcctttc gattgagccc gcccagcggc cgtcgatcga agaggtgctg
     2461 ctcgccggcc gcgacgacat cggcacgcag ccgatgcacg gggccgatct gccgcgggca
     2521 cccggcgagc ccgtcggcgg cgcaatcggc cgagccatcg ccggcgccgg catggccgct
     2581 gccgcgggcg ctgccggcat gattgccgcg gcggcgcaag cggtctcggc tggcttgtcg
     2641 ggcttgaccg gtggcgaagc cggcgccgcg gccagcgctg cgccgcagcg ccgcgccgct
     2701 gcctccggcg gatcgcaggg ccccaactgg atcgagcgcc tcgcccagtg ggcccgccag
     2761 aagcaacagg ccatgcggca gaacatcgac cagatgcggc acagccagat cgagcggctg
     2821 ctgagcgcct tgcagaaatc gcccgatttg ggcctgcggt tcgccctccc gctcaccagc
     2881 ctcggcgccg cccgcggtca cgcgccgccc ggcgcccgcc tgatcgagcg gaatatcaac
     2941 ttcaacttga atcgcctggg cggcggcggg ccggtcgatg tctgggactt gcccgacgac
     3001 tatcgccgca agctcgccct ccgctaccgc gagcttgcca atcgcgaaat cagtttgggc
     3061 cggcatcgcc gggcggctta catcctggcc gagctgctcg gcgatctcac ctccgcggcc
     3121 gccacgctgg ctgagggcca gcactttcgc gaagcggctc agctctacga aacccgcctc
     3181 aaccagccgc tcgccgccgc ccgctgcctg cagcggggcg ggctcctcac cgaagcggtc
     3241 gccctgtacg agcgactgag cgagtgggag accgtcggtg agttgtacga gcagctcgac
     3301 cagcccgacc gcgcggcgga agcctttcgc cgggctgtga cgcgccgcat ctcggctttc
     3361 gaccatctgg gcgcggcgaa gctgctcgaa acgaagctcg ccgtgccgga cgaagcattg
     3421 acgacgctgc tcggcggctg gaacggctcg tcgcaagccg aacgctgcct ggacgaagcc
     3481 ttcgacctgc tcgcccgcca tcagaaaaac gaccgcgctc ggtcgctcat cgacgagctg
     3541 tcaaccaggc cggcgaagcc ggtagtgcta accggcggtc tggctcgcct ggcgacgaag
     3601 tttcccgacg ccggtgtccg cgagaccgca gccagctcgg cgcgccgcct cgtctccgcc
     3661 cacctgccca ctgcagctcc gtatgaatct gaaagcttgc tgcggtcgct gggaagcctc
     3721 gtccctgccg atcgattgct ggagcgagat tgccgccgct accgcgagca gcaaatcgaa
     3781 aaggaccgcc ggcggatcga gcaagcgtct cgccggcagc cgcgaagcaa gctccccacc
     3841 cccatccgca cgttccgcct cccgcccggc gagtggaaag ccgcggtcgg cgcgggcgag
     3901 gagttctacg ccgccggcat ccatgccgag tcactgctgg tggtccgcgg ccgcttcgac
     3961 ggcgcagtgc agctccccat gggcgacgtc tgcaagctca ccgccgagca ggccgccgcg
     4021 ccggtcttcc ttgccgccga tccccgcggc ctggcgttta cgtacgtgca tgttcctccg
     4081 gacaagccgt ggcgcgatct gcgctttgcc gcgaccgaca agtaccagcg ggcgatgcaa
     4141 gtcagcccca tctggggcgg ggagaaaccg ctagagggcc tgatctattc ctctggcggc
     4201 tcgctcctgc tcctgacgct ccccgagccg ggaatcgccc aggtcacgcc gtacgatcct
     4261 cagctccggc tgcggggcgt ggtgtcgttg acctattcga tcgacgccct ggactcggac
     4321 gacgacatcc atcgcccgct cccgtttttc gcccgcggcg attcgctcta tctggcgatc
     4381 ggctcgcggc tgatcagcaa gcgcggcggg caggaatcgt cgcggctata ctcgccgatc
     4441 cgctccatca ccggctcgcc actccacacg cggccgcgaa tcgtgctggc ctgtgaggac
     4501 ggcggctacg ttcagtgggg tgaatcggca cgcagcccag ccacacgctt cgcccaggat
     4561 atgcaccagc cggttgtggg cctgacccgc ggcggctggc tggccgcgtc cgtccggggc
     4621 gagctcgaag tccacagcac cgaaggcggc cggctttcgt tcatcggccg cacgagcgcc
     4681 ccgaccgaag acccgctcgc catccttccc accggcgaca ccagtcgctt cgctctcgtc
     4741 acggcaagcg gcctggttac cgtctacgaa gtgccgggtt gacgtcgggc gttcagcgcc
     4801 aatcgcctcg cgatgttcca cgtattgcga atccagtctg aagcgcccgg ttagcgagac
     4861 atagcgacaa ccctgggatc tccaatcagg tgcagattgc attggagatc attcaatgac
     4921 gtttcacagg cggaatgcgc aaccaatcgg ggcgcgaaag caaccggcag tcagtcgtcg
     4981 gcaggcagtg agttggagct tgattgcagc ccgcagaaac cactaggcac tttgtggccg
     5041 accggcgcgg aggcggattt cttcttccag gcgctggatg gcttgcttga gctgtgcgtc
     5101 gatcgtggcg gggtctttca gggcgttggc ggcggcccgc tgttcggcct ggcggatggc
     5161 ggggtcatcg gattcggcgt cgggaacggg gactggcgga ttcgctacat ccaaagaggg
     5221 cgccgccagc tcgtccgggg aaggcgtcgg cggcgatgga agcggtggag actcaggcgc
     5281 agggagttcg gccggcgccc tcggcgcaga tgttttcgcc gcgatcaagt cctcatagga
     5341 cgtgacatca cgccgccggc gggcgaggac gaccgtttcg aattcctgat ttgtgaagtt
     5401 gacttcttgg ccgggatcgg gccgcacgcc ccaatcgtcg gtgtctttgg ccccttcgcg
     5461 cttgtggatg tcgtgaccgc tcggccgcca gtagctgccg atcgtcaggc ggagtgcgct
     5521 gcgcccgcct tcgagagaga tcacgttttg tacggtccct ttaccccagg accgctggcc
     5581 gacgaccatc gcccgctcgt gatcttgcag gcaggcggct acgatttcgc tcgcgctggc
     5641 cgagtactta tcgatcagga cgacgagcgg aacgtcgtcg ggcaactcgg tgccgagcga
     5701 cgcttcgcgg cgctcgcgct cgactccgtt gcgtccgcgc gtcgtgacga tcacgccgtg
     5761 gtcgagaaac gcgtcgcaaa cctcttgcgc cgcgttgagg agtccgccgg cattgccgcg
     5821 caagtcgatg atcaaaccgt cgatctgctg gccgggctcg cggaagcttt gtagcgcggt
     5881 tttgagctcc tcacccgttc gttcgccgaa gttgatgatc cgcacatagc cgatccgcgg
     5941 ccgatcgacg agatgataca cccaggtgcc gtcggcgcgt cgggcatcgc ccaacaccga
     6001 ttcgatggga atgttcgccc gagccaactc atactcgacc ggttctggct cgctggcgtg
     6061 ttggatgcgg accttcacct ttgagccgac tcctccccgc atgagcttga ccgagtcggg
     6121 cagcgacatc ccttcggtgt cgtggtcgtc gatcgccagg atcgtatcgc ccgatcgcag
     6181 gcccgctttg tacgccggcg tgcctacgag cgggctgagc accgtcagcc gcccggtttc
     6241 ttgattgacc tcgacaacga tcccgatgcc gccgaattcg ccgtccatcg tctcctggaa
     6301 ctggttgtat tcgtcggggg gcgtatagcc cgaataggga tcaagcttgt tcatcatgcc
     6361 gtccatcgcg ccttcgaaga ggattcgcga atcgaccggc tcgatgtact ctcggtcgat
     6421 gatggacagg gcctcgctga gggtcgcgcc gtaacggctc atcagcgcct ggcgatagca
     6481 aaccagcgac agcaccgacg aaatgagcag gatcgtgaga ttgtggcgcg ccatgaaagc
     6541 acttacgcga gcaggtcaag gaaggagtac gcgattcatc ctggaggcgg ggtgggctgg
     6601 tgtcaacgcg gctgggctgc gagcgctgga gagaatcgct ggcaagaacg cgccagcagg
     6661 cactggataa tcggctgctc gattctgaat aatcggcaat ttccgcgtag attcgcaaat
     6721 tgaccaaccg atgacaccag ataccgcgac agatcgactg ctcgaattat tcttggggcg
     6781 tgctaaccgg catgtggatc acactggcca tcctggcgac cgcggcgctg gcgctcagtg
     6841 cagtgctgtc gttgcacaaa tgggaatcgc ggcggttcgt gcgaaggggg ctgaccaatc
     6901 gggtgcagcg cgatgaggcg atccaagcag tcctcatcgt cccttgcaag gggatcgaca
     6961 ttggacaggt ggagaacttg cgggcgctct tcgagcaacg ccagatcgac taccaactgc
     7021 ggctagtcgt ggaagacgag cgcgattccg ctgcggagct gattgcagca ctgcaagccg
     7081 agtttccgca tgtccgctca gaactgctga tcgccggacg ggccatcgaa agcggccaga
     7141 aggttcacaa tctgctgata gcaacggaga acctgcctgc ggatgtgaat gtgttggcat
     7201 ttgccgattc cgatgcgcgg cccggccccg attggctcgc ggcgctcgta tcgcggctga
     7261 atgagctgca gattggcgcg cggacgggct accgctggat ggtcccggaa cgaatgacgt
     7321 ggccgaattt gctcgtgtat agcatcaact gcagcgtggc gagcatgtac agccgccacc
     7381 gcttgaatgc cgtatggggc ggggcgtggg cgattcgccg cgagctattc gagcaggtcg
     7441 gcattcgcga ggcttgggcg ggaacgctta gcgacgacct cgtcgtgacg cgcgtgctgc
     7501 gggcctcggg tctgcagatt gaattcgagc cgcgctgcct gacggcttcg ccggtggata
     7561 tgaccgcgcg cgaggcggtc aatttcctgc gtcggcagtt cctgctggtg cggcgctatg
     7621 cgcccctgca ctggtggttg gcgctcgcgg gcagcagcct gttgcagggc ggattatggg
     7681 gcgctgcggt actgaccttg ggatcggcaa tcgccgggca ggcctggtgg ctgccgggcc
     7741 tgatgggcct gacgctgtac gtcttagcca tgtttcgcgg ctggcttgga caatcggcat
     7801 ttcgagcgcg cctggctggc catgaatcga aattggcctc gccgcggcgg tttgatattc
     7861 tgctcggccc gctggcgggc gtttgcggtt ggctgacgat gctcagctcg ctcgttggca
     7921 gcagcatgac gtggcggggc atccgctact ggatttcgcc cggcggccga gtgctgctgc
     7981 tgggacgcgt cgtggaggcc ggatcgctgt ccggtgcgat tcgcatgccg ggccgatcgt
     8041 ccggcgacaa ggccctggaa atccagcgcc gtgccctgga gcgaggtatt cggctgcacg
     8101 acgcagccac ggaagtgccg cgagatcgca aagccgcata ggcgcgcagc ccgcgtctaa
     8161 cccgacctct gcgccaggaa acacaatggc cttggcttcc ctgacaccgg caactcgacc
     8221 tgtcggcgcg accgatgccg cgccgctctt gtcccaaaat ctcgatcacg agtcgcaggc
     8281 gctggacgag tttcgccggg ccaatgccga cctgtttggg aagagcgatc tctggctggc
     8341 gctgcatttg gctgtagtgc tgacgctgct cggcctgggg acgtgggcga tcgtggtcgt
     8401 gccgctcgtg tgggtcaaga tcctgctggg gcctgtgaac gcgttcctct ggttctgcct
     8461 ggggaacgtg acggtccacc atcaccacac gcaccacaat gcggccaaga gcgcggtatt
     8521 caaacggctc ttggacggac tgcatttcct ggtggtgccc gacggcggca ggggcctgaa
     8581 gcgctaccgc cgcgcccaca tgaaccatca cctccatccc ctgtccgact cggacgtcga
     8641 ccatcgctac gcccagaagc actaccagaa aatgtcaaag agcgtgtgga cgagaatcgt
     8701 ctatttcctg gagctgacgt tcatcggcgg gcacgtgccg gggcaaaagg acgaagctta
     8761 tctcaatagg gtgccaatcg cgaagtggaa ccttgcagaa tatgaaaagg tgaaggagcg
     8821 cgaagtccgc aaagccaaac gcaattccct cattgcctgg ggcatttttc tggttgtgct
     8881 gttcgtcttt ccgccgctgg cttgggcctg gatttacccg ctgattctgg tgcggaattg
     8941 gaacggcttt ttgggccagt tccagcacta tgacgagaag ctgctggcgt ccggccgcag
     9001 caagcacaac cgcacgctga cttttcgcat tcccggctgg ctgaactatc tcgccggtgg
     9061 cgaaatcagc gggcatttca tccaccacat ctatccggac atgccttact accacgtcga
     9121 aagcgcccgg cggcgactgc tgcgccagcc ggagttggtc aggctggtgg ttaaccatta
     9181 ggacggccga cgctcgctac ggcaggttcg tcgattgcct tcgttggggc gcgcggtgat
     9241 acttggatca gcgaatccca cgatactacg agtccagcca tgatcgtccg ctctccccga
     9301 cgtccttcaa gaatccactc gtcggagacg ttcgccgatt tgctccagcg cctgggcgat
     9361 gttgctccag agcgaattcg cctcgatccg tggccgggaa aggtgacgga cgaggatgtc
     9421 gccgaatcga aggaccgatt cggatgcttg tgcgaactgg tggacggcgt gctggtggag
     9481 aagattatgg gagtacctga atcggcgctg gccacagaac tgtcacggat gatcaagaac
     9541 ttcctcgacg aacatccgct cggaatggtg tgcgggccgg atggcccggt ccggtttcag
     9601 tcgcggttga tccgcatgcc ggatatcagc tttttctcgt gggaccgaat cccgggcgac
     9661 cggatggaag aggacgccat gctcggaatc gtcccggacc tggttgtcga agtgatttcg
     9721 cctgggaatc gcccgggcga aatgcgtgtc aagctgcagg agtactttca ggcgggtgta
     9781 cgactggctt ggtacattca tttcaagcag cgcaaggctc agatttacac gagcatcgac
     9841 gacgtgaccg acgtgagcct gaacgattgc ctcgacggcg gcgacgtgct gccgggattt
     9901 cgcgtgcggt tgcagacggt ctatgaccgg gcctttccaa cgcggccgaa gaagaaacgc
     9961 ggtaaatgag gcggctaagc ggctcggcgt tcgagtgaag caggtgtcgc cgattcgtca
    10021 acatacccgt gctgccgcat gaaggggccc attttttcgg cgatttgtgc gcgagcgccg
    10081 tcggcgagcg gctggtggcg gtttttctcg tagccttgca ggccggcgag atagcgctcc
    10141 aggcggcggc gataatcggc gctgaactca ataccgagct gttcgtaaat cgcttcgatc
    10201 ccgccgatcg gatcgcgttc gaggtcctcg aagcggaggc gggcgacggc gtttgcgggc
    10261 aggcgctgtg catcgtcggc aaaggcctgc tccatggcgc gatagttgtc gagaaagcgg
    10321 gcttcgtaag aattccgctc gtcgggatcc tgcaactggt tgcagacgtg gccttcacga
    10381 gcgagatgga tattcgagcg atagacgacg tgcggatgac ggcagatgtg aacgaacttc
    10441 gcgccaggaa acatctcgcg gagaatgccg acgcgggccg tgttgtaagg gtttttgagc
    10501 agcaggcgct tctgtgtcca cagcgaatgc ttgcggagca gcagcaggta ctggtgcttc
    10561 cacacctggc gctcggctgc gggcaggcga tcggggaaga tgtagcggtc gaagtgcggc
    10621 cagaggcgcg ggaaggtcat accccagtgc ggagacatca ccgagcagcc ggccaggccg
    10681 aattcttcct cctgcggcgt gaacatgctg aaccgcacgc catccatcgg acgcttccag
    10741 ggcatgaaca ggcagaggag cggggtgacc agccagccgg acaacaggaa gccggcgccg
    10801 ttcatcgtct ggtagttccg cgccgtacag aactgcggat cgagggcgag gaggttgtgc
    10861 aaatgcgtgg taccgctacg gtgggttccg acgacgaaga tcggatcaac gacgcgctta
    10921 tttcgcaaga ttagcggcag cagcaggcgc tcgggcagcg agaggagcgt gttgaccgcc
    10981 gacacgacca gcaccgccaa gagccgcggc acgtacttga agccgacgcg aaagcgggcg
    11041 gcgatcaata gcggaatcca gacgtcgagc ggcgccaggc cgttggtgtg cgagaacggg
    11101 aaaatgcgct tgaaatctgc atcgatgccg gagggcggaa gctgcggctt tccctcgcca
    11161 tggcgccaga caagcaccag aacgacggtc acggcggcgg ccaggaccag gagcatgacc
    11221 gggccttgcc gcggatcggc tgccggcagg agccagtcga accacggcga atcgggctgc
    11281 ggcgcgagga ttcccgtctt gtgcaccatc ttccgggcga tgagccagcc cgcgagcagc
    11341 cccgacgaag tcagaatctc gcccgtcttc agccgcaacg cggccagcag ggcggccgcc
    11401 aggaaaatac ccacaacaag ctgccgcgaa tcgccgcgga tcagcgaggc ggggtcgttc
    11461 atctggtgca gcacggtgaa caacgccgcg gccagcgcta cg
//
LOCUS       DASUGB010000852         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1204969, whole genome shotgun sequence.
ACCESSION   DASUGB010000852 DASUGB010000000
VERSION     DASUGB010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204969"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFV87_16175"
     CDS             <1..611
                     /locus_tag="VFV87_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105357.1"
                     /translation="QAELFLSREHALLEDQERQVVSDLSDAISELDRAYELARTQYNR
                     RMAALRQLETLGVLLQQADNVEKPRLLDLQLDAQRRLADSESQFYRSLAEHEVAIKNV
                     HLEKGTLLDYNRIFLAEGPWPGKAHADAAQRARLWIGAPHLENYVRDDRIVSAGAFPQ
                     DSLPPGTAEELQRDPPTPQPAPVLESQPLQPLPPVDEGLSPQ"
     gene            898..>3373
                     /locus_tag="VFV87_16180"
     CDS             898..>3373
                     /locus_tag="VFV87_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105358.1"
                     /translation="MTRLEDRIVLDGAAPVTDLNPGTDGSNPAEQVEYNGSVYFSADG
                     IDSNGDAVGRELYRLDADGSITLVDDINAGTAGSDPSDFRILDGELYFAATTATTGRE
                     LYKLDANGNVTLVSDINPGPASSNPEFLSEFNNKLYLAATTPTSGRELYVVNRGGNAS
                     LVADINPGSASSNPSDFFEFEDNLYFVATGPSGRILYQQNPSGTSDPLAVSLGTGVTN
                     PQGFTEFNDAIYFSALDPADGRELFKLTVDNRNSSTVAKVANLDGTSASSSPDGFTVF
                     DNHLYFAATAANGCELFQLDSAGNIVQVDIAPGTASSSPAGFTEFDGHLYFAATVDGQ
                     RGLYRLDTSGAIPTAVPVPLPAGVSLLEDAVFYNLGTELYFAADGPQGRELYRLTLAG
                     VVELAADINAGPGSSNPAEVRLFGGQIYVVATEPSVGRELFVLLRSPSTLTVVGDTLV
                     FKDESGDEDNRLVIVSDGTNLTIRDENGHDIALLTPLAGATGSGTNEVTIPLASLVGV
                     SGLAINTRGGNDSVTFDLSANADAILGQFVVSTYDGGDNDPDRPGDRLRFIGDGITNS
                     TYTPDAATTGSGVVIVSSSAQTMTFEFDDLEPVEFTGMAEARLVTPITTDGDDVLIVS
                     EGVDSVDGLFDVLIVSGTVGGVAIESGYFFGNDSVVIVTGSGVDGTDQVTIVGADNNH
                     FNANLTIDTGAAGQDEVLISGDVNLAGQLTIHTFLLDVRAIVTLGGDAWLAARDDILF
                     SSAASLVAEPGAGIDVTLTADSDGVDGGAITMADGALINAGSGTIGLTATGDITLGRL
                     VTTSGSSLAVVVQSTAGRILDGGDSGG"
BASE COUNT          634 a         1057 c         1029 g          653 t
ORIGIN      
        1 gccaggccga gttgttttta tctcgtgaac atgctctcct cgaggatcag gagcggcagg
       61 tcgtcagcga cctgagcgat gcgatctccg aactcgatcg ggcctacgag ctggcgcgaa
      121 cgcagtacaa ccgccgaatg gcagcgcttc gccagctcga aacgctcggc gtcttgctgc
      181 aacaagcgga caatgtagaa aagcctcggc tactcgacct ccagctcgat gcccagcgac
      241 gcctggccga ttccgagagc caattctatc gctcgctggc ggagcatgaa gtcgccatta
      301 agaacgtgca cctcgagaag gggacgctgc ttgattacaa ccggatcttc ctcgccgagg
      361 gaccctggcc aggcaaggcg catgcggatg ccgctcaacg ggccagactg tggatcggcg
      421 ctccgcacct tgagaattac gtccgcgacg accggatcgt ttcagccggg gcatttcccc
      481 aggactcgct gccacctggc acggcagagg aactgcaacg tgacccgccg actccgcaac
      541 cggctccggt cttggaatct caacccctgc agccgttacc ccccgtcgat gaaggtctct
      601 ctccgcagta aacggagaat cgagcgccct gtttgacagg ttcgcgaaat ccaatgacaa
      661 tgactaggct attttggcct acctgtcgct tgaccacttc gccgatggat tcgcgaatcg
      721 cgcgctttgg ttgcgccccg gtagccgtgg agtttcctgc cgtctccagg gctgcgaacg
      781 atgcctctac gtgcttggac cgcgcgtctt tatcgctctt ggagtgcgcg ccggcgtcgt
      841 gttgttgcgc ggcaatcgcg gcggcaattt cttgacagcg attttatccg actgggattg
      901 acgcggctgg aagatcggat cgtgctcgat ggcgccgcgc cagtcaccga cctcaacccg
      961 ggcacggatg gatctaaccc ggccgagcag gtcgaataca acggcagcgt ctatttcagc
     1021 gccgacggca tcgactcgaa tggcgacgca gtcggccgcg aactctaccg attggatgcg
     1081 gatggcagca tcacgctggt tgacgacatc aacgcgggca cggcgggctc cgacccatcc
     1141 gatttcagga tcctcgacgg tgagctttat ttcgccgcca cgacggccac cacaggtcgt
     1201 gagttgtaca agctcgacgc caacggcaat gtgacgctcg tctcggacat caatcccggc
     1261 ccggcgagct cgaatcccga atttctcagc gaattcaaca acaagctcta tctcgctgcc
     1321 acgacgccta ccagcggccg agagctatat gtcgtcaatc gtggcggcaa cgcgtcactc
     1381 gtcgccgaca tcaaccctgg ctcggccagc tccaatccat ccgacttttt tgaatttgaa
     1441 gacaatctgt actttgtcgc aaccggtccc agcggtcgca tcctctatca acaaaatccc
     1501 agtgggactt ccgacccgct ggccgtcagt ctcggcactg gcgtgactaa cccccagggg
     1561 tttaccgagt tcaacgatgc gatctacttc agcgccctcg atcccgccga tggtcgcgag
     1621 ctgtttaagc tcacggtgga caaccgcaac agttcgaccg tcgcgaaggt cgccaacctc
     1681 gacggcacgt cggccagttc ttccccggat ggattcaccg tttttgacaa ccatctgtac
     1741 tttgccgcca cggccgcgaa tggctgcgag ctgttccaac tcgactcggc gggcaacatc
     1801 gtgcaagtcg atatcgcgcc ggggacagcc agctcttcgc cggccggctt cacggagttc
     1861 gacggacatc tctatttcgc cgcaacggtg gacggccagc gcggccttta tcgcttggat
     1921 accagtggcg cgatccccac agccgtcccc gtgcctctgc ctgctggcgt ctccctgctc
     1981 gaagatgcgg tgttttataa cctggggacc gagctctatt ttgccgcgga tggcccgcag
     2041 ggtcgcgaac tttacaggct gaccctggcc ggcgtcgtcg agttggctgc cgacatcaat
     2101 gccggacctg gaagctccaa tccggctgag gtgcggctgt tcgggggcca gatctacgtg
     2161 gttgccacgg agccgtcggt ggggcgcgag ctgtttgtgc tgttgcgaag cccctccacg
     2221 ctgaccgtcg tcggagacac gctggtgttc aaagacgaaa gcggggacga ggacaatcgg
     2281 ctcgtgattg tgagcgacgg caccaacctg acgattcgtg acgagaacgg ccacgatatc
     2341 gccctgctca cgcccctggc cggagccacg ggcagcggca ccaatgaagt gacgattccc
     2401 ctggccagtc tcgtcggggt gagcggtctg gcgatcaata cgcggggcgg caacgactcc
     2461 gtcacctttg atctgtcggc gaacgcggac gcgattctcg gccaattcgt cgtttccacc
     2521 tacgacggcg gcgacaacga tccggaccgg cccggcgatc gcctgcggtt cattggcgac
     2581 ggcattacaa actccaccta cacgcccgac gcggctacca ccggcagcgg cgtggtcatc
     2641 gtttccagct cggcgcagac gatgacattc gaattcgacg atttggaacc ggtcgaattc
     2701 acagggatgg cggaggccag gttagtcacg ccgatcacca ccgacggcga cgatgtgctc
     2761 attgtttccg agggcgtgga ttcggtcgac gggctcttcg acgtcttaat cgtgtcggga
     2821 accgttggcg gcgttgcgat cgaaagcggc tatttctttg gcaacgattc cgtcgtgatc
     2881 gtcaccggaa gtggcgtcga tgggaccgac caggtcacaa tcgttggcgc tgacaacaac
     2941 cacttcaatg ccaacctgac cattgatacg ggcgcggcgg gccaggacga ggtgctgatc
     3001 agcggagacg taaacttagc cggccagctc accattcata cgttcctgct tgatgtgcga
     3061 gcgatcgtaa cgcttggcgg cgatgcctgg ctcgcagcga gggacgatat tcttttttct
     3121 tcggcggcaa gcctggtggc ggagcccggg gcgggcatcg acgtcacgtt gaccgccgac
     3181 agcgacggcg tagatggcgg cgcgatcacc atggccgacg gtgccctgat taacgccggg
     3241 agcggcacga tcgggctgac ggccacgggg gacatcacgc tggggcggct ggttacgacg
     3301 agcggcagca gcctggcggt ggtggtccag agcacggcgg gccggatcct ggacgggggg
     3361 gacagcggcg gcg
//
LOCUS       DASUGB010000853         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1343476, whole genome shotgun sequence.
ACCESSION   DASUGB010000853 DASUGB010000000
VERSION     DASUGB010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343476"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFV87_16185"
     CDS             complement(<1..956)
                     /locus_tag="VFV87_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5105359.1"
                     /translation="MYSYGAFRWSTLMQLGLLLIVPSIALAEASKERPNVLFIAVDDL
                     NDWTGCLGGHPQAKTPNIDSLAARGVNFGKAYCACPVCNPSRTALMLGLRSSTTGVYS
                     NGNNRRDSKVAMSVTPLNTYFHEQGYYVAGAGKIYHGDGDQFGEWDDYAEIRGGDLPP
                     AAGHDGGVGGIRFAPVDAPDEDMHDYKTVTYCIEQLQKKHDKPFFLACGLHKPHMAWN
                     VPQKYYDLFPLDSIELPKTLADDLADIPAAGVRMAKPEGDHAQMLKSGRWKEAVQGYL
                     AAGAFCDAMIGRLMQAFDKSVYKDNTIVVFWGDHGWHLGEKQ"
     gene            975..1205
                     /locus_tag="VFV87_16190"
     CDS             975..1205
                     /locus_tag="VFV87_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105360.1"
                     /translation="MLPDMSDTPDDPELRKFLASQTPPAPDRDAPEMQRLFDHTRNAP
                     PRTYRLDAFAKAGIGVFVILIILRILIKFFLN"
     gene            complement(1221..>2580)
                     /locus_tag="VFV87_16195"
     CDS             complement(1221..>2580)
                     /locus_tag="VFV87_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5105361.1"
                     /translation="GNSERGEVRFERRAGLKRPEKDEDLAFLPVTDLAALIHAEQISS
                     VELTKLYLARLKRFDPLLKCVVTLTEDTAMQQAERADREFAAGKYRGPLHGIPWGAKD
                     IIAWPGYKTTWGAGVFQDQTVELKATVASRLEEAGAVLVAKLSVGSLALGDEWFGGMT
                     RNPWNPEEGSSGSSAGSTSAVVAGLVGFAIGSETLGSIVSPCKRCSASGLRPTFGRVS
                     RHGCMALSWTMDKLGPICRSIEDCALVFGAIHGYDGHDQTAVTRPFDWPAKRELSSLK
                     IGYFEDGPPLEERPETAVLRKLGVTLVPIKLPSDLPAGALTLILNTEAAAAFDELTRM
                     GIRDGIGHWATTFVKGQFVPAIEYLRANRIRTLLMRQMDEAVSSVDAYVGGDDLVITN
                     LTGHPTAVVPFGNRADGKNGQPGTITFTGRLFGETDLLALAHAFQQAGSDHQRHPPLN
                     DQ"
BASE COUNT          425 a          800 c          856 g          499 t
ORIGIN      
        1 tgctgctttt cgcccaagtg ccagccgtga tcgccccaga aaacgacgat cgtgttgtcc
       61 ttgtagacgc tcttgtcgaa ggcctgcatc aggcggccaa tcatcgcgtc gcagaacgcg
      121 ccggcggcca ggtagccttg caccgcctcc ttccagcggc cagatttgag catctgcgcg
      181 tggtcgcctt ccggcttggc catccgcacg ccggcggccg gaatgtcggc caggtcatcg
      241 gcgagcgttt tgggaagctc gatcgaatcg agcgggaaca ggtcgtagta tttctggggc
      301 acgttccagg ccatgtgcgg cttgtgcagg ccgcaggcga ggaagaaggg cttgtcgtgc
      361 ttcttctgta gctgctcgat gcagtaagtc accgtcttgt agtcgtgcat gtcctcgtcc
      421 ggcgcatcga cgggggcgaa gcgaatgccg ccgacgccgc catcgtgccc cgccgcgggg
      481 ggcaggtcgc cgccgcgaat ctcggcgtag tcgtcccact cgccgaattg atcgccatcg
      541 ccgtgataga tcttgccggc gccggcgacg tagtagccct gctcgtggaa ataggtattg
      601 agcggcgtca cgctcatcgc gaccttggaa tcgcggcggt tgttgccgtt gctatagacc
      661 ccggtcgttg agctccgcag gccgagcatg agcgccgtgc gcgagggatt gcagacgggg
      721 caggcacaat aagccttgcc gaagttcacg ccgcgggccg ccagggagtc gatgttgggg
      781 gtcttcgcct gcggatgccc gcccaggcag ccggtccagt cgttgaggtc atcgacggcg
      841 atgaagagga cgttgggccg ctctttcgaa gcctcggcca gtgcgatgga aggcacaatc
      901 aggagcaaac cgagctgcat caacgtggac caacgaaacg cgccgtagct gtacattgct
      961 ggaattctcc gtgtatgctt ccggatatgt cagatacccc cgacgatccc gaacttcgca
     1021 agttcctcgc cagccagacg ccgcccgcgc cggatcggga cgcccccgag atgcagcggc
     1081 tgttcgatca cactcgcaat gctccccctc gcacttatcg cctcgacgct tttgcgaaag
     1141 ccggaattgg tgtgttcgtt atccttatca tcctccgcat tctaatcaag ttttttctaa
     1201 actgatttgc ggcgccctat tcattgatcg ttgagcggtg gatgccgctg gtgatcgctg
     1261 ccggcttgct ggaaggcgtg ggccagggcg agcaggtcgg tttcgccgaa caggcgaccg
     1321 gtgaaggtaa tcgtgccggg ctggccgttc ttgccatcgg cgcggttgcc gaaggggacg
     1381 acagcggtgg ggtggccggt gaggttggtg atcaccaggt cgtcgccgcc gacgtaggca
     1441 tcgacgctgg aaaccgcctc gtccatctgc cgcatcagca gcgtgcggat gcggttggcc
     1501 cgcaggtatt cgatcgcagg gacgaattgc cctttgacga acgtcgtcgc ccagtggccg
     1561 atgccgtcgc ggatgcccat ccgcgtcagt tcgtcgaacg cggcggcggc ttcggtgttg
     1621 aggatcagcg tgagggcgcc agccggcaga tcgctgggca gcttgatggg gacgagcgtg
     1681 acaccgagct tgcgaaggac ggcggtttcc ggacgctctt ccagcggcgg gccatcctcg
     1741 aaatagccga tcttgagcga tgagagctcg cgtttggccg gccaatcgaa cggtcgcgtg
     1801 acggcggtct gatcatgacc gtcatagccg tggatcgccc cgaaaacgag ggcgcagtct
     1861 tcaatcgagc ggcagatcgg gccgagtttg tccatcgtcc acgagagggc catgcagccg
     1921 tgccggctga cgcggccgaa ggtggggcgg agtccgctgg cggaacagcg cttgcagggg
     1981 ctgacgatgc tgcccagcgt ttcgctgccg atcgcaaagc cgaccagccc cgccacgacc
     2041 gccgaggtcg agccggccga cgagccgctg gagccttcct ccggattcca tggattgcgg
     2101 gtcatgccgc cgaaccattc gtccccgagg gccagcgaac cgacgctcag cttggcgacg
     2161 agcacggccc cggcttcttc cagccggctg gcgacggtgg ctttcagttc gaccgtttgg
     2221 tcctgaaata cgcccgcgcc ccaggtcgtc ttgtagccgg gccaggcgat gatgtccttc
     2281 gctccccacg ggatgccgtg cagcgggccg cggtatttgc cggcggcgaa ctcgcggtcg
     2341 gcccgctcgg cttgctgcat ggccgtgtct tcggtgagcg tgacgacaca cttcagcagc
     2401 ggatcgaacc gtttcagccg ggcaagatag agctttgtca gctcgacgga tgagatttgt
     2461 tcggcgtgga tgagcgccgc cagatcggtc acgggcagga aggctaggtc ttcatccttc
     2521 tcgggccgct tgagacccgc tctgcgctcg aaccgcacct cgccccgctc cgaattgccg
//
LOCUS       DASUGB010000854         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1482708, whole genome shotgun sequence.
ACCESSION   DASUGB010000854 DASUGB010000000
VERSION     DASUGB010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482708"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFV87_16200"
     CDS             complement(<1..689)
                     /locus_tag="VFV87_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105362.1"
                     /translation="MARTCGQCGLVLAALGVCAAASGQTTIQFPRTSTPPATTTTTAP
                     GIITAPAPGATTTVNPYTGGSFNPGPFDPYSTNGASAYQFWSNTPPSNTMGVFAQPPP
                     GSLGTTSPLVTQPPVASPYGPPATAPYTPGAPQFVFPNGMGQPWDLQQSVRLIQDLRM
                     RYTWVEGGDDPTDLDINDIETAVSFTWPAFLWTNQPLFISPAFALHLWDGPKGISADL
                     PSKAYSAYLDL"
     gene            910..2559
                     /locus_tag="VFV87_16205"
     CDS             910..2559
                     /locus_tag="VFV87_16205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105363.1"
                     /translation="MTPDPNNPYSPPQAEPARPKPDYEFGPMTRVRFLVVGMCVLMSV
                     LLYIDRFATTPTTDTILSELKLSKTEFGDAIGAFFLAYALMQIPSGWITDSFGARWML
                     ALYVLCWSLATIGLGFAQGLAAVWTMRLVLGIMQAGAYPTAAGLLKRWIPYTERGLAN
                     SIVSMGGRCGLLLSLVLTVPLMRAVGGVTGWQLGNWRAVFAFYGGLGLAWAAAFVWLF
                     RDWPRDHPRCSPAERRWISGDAAEAKVKDDPGSQGRVWRLGLFYALLVGAILGLILVL
                     VAARDLLIAQFGPSLTQLTGSEAGMGAIVNTVPELGGLIGTLALCFGANLLLDRFFPA
                     DRHLQLPFRAMAASKEVWLMCAINFFVNIGWILLATWLPQYLIENHGSTLTATIGDKV
                     FVAALITAVVQVAAMCGGLSGGRATDVFVRRFGPTWGRRLPGMCAGLLVCGMYLVVPQ
                     LADLWLFVAMMIVIAYTIDFGLGATWASYQDIGGRNVAAILGVGNMCGNLGAAYFGRL
                     IGILADHNQWNAVFYIAAGAMLAAASCWMLFDASRAVVQER"
     gene            complement(2891..>4047)
                     /locus_tag="VFV87_16210"
     CDS             complement(2891..>4047)
                     /locus_tag="VFV87_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX5105364.1"
                     /translation="VEPARWYHHCDKLGLLVWQDMPSGDKHAPWRPTGEHNGEEIQRS
                     KESAENFKNEWSEIVKDLSHFPCIVVWVPFNEAWGQFDTAGITKFTQELDPTRLVNCA
                     SGGNDFPVGHIVDLHRYPGPTIPKLEVSRAAVLGEYGGLGLPLEGHTWQDKNNWGYRS
                     FKTREELNSAYEQLLFKLRPLIGQGLAAGIYTQTTDVEIEVNGFMTYDREVIKFDAER
                     VAELHQKLHQPPPKEVVVVPTSRDEPQTWRYTTAKPAEGWERPDFDDASWKEGQGGFG
                     EPTTPGSKVRTEWKTADIWLRRSVELPGNKLDGLALLIHHDEDTEVYLNGKQIAAVKG
                     FVTDYTLVPLNEKTLAATLQDGANTLAVHCHQTGGGQYIDVGFVRLIEAE"
BASE COUNT          641 a         1322 c         1276 g          808 t
ORIGIN      
        1 tgtaaatcga gatacgcgct gtacgccttc gacggcaggt cggcggagat ccccttcggc
       61 ccatcccaca ggtgcagcgc aaatgctggc gaaatgaaca gcggctggtt cgtccacaag
      121 aaggcgggcc aggtgaagct gacggccgtt tcgatgtcat tgatgtccag gtcggtcggg
      181 tcgtcacccc cttcgaccca cgtgtaacgc atccgcaggt cctggatcag ccgcaccgat
      241 tgctgcaagt cccacggctg gcccatgccg ttgggaaaga cgaattgcgg tgcgcccggc
      301 gtgtacggag ccgtcgcggg cggaccataa ggcgaggcaa ccggcggctg cgtaaccagc
      361 ggcgacgtcg tgccgaggga acctggcggc ggctgagcga agacgcccat cgtgttggac
      421 ggcggcgtat tcgaccagaa ctgataggcc gatgccccgt tggtcgagta cggatcaaat
      481 gggcccggat tgaacgagcc gcctgtgtag ggattgacgg tcgtcgtcgc gcccggcgca
      541 ggcgccgtga tgatgccagg agcggtggtc gtcgtggtcg ccggcggggt ggacgttcga
      601 ggaaactgga tcgtcgtctg cccgctggcc gcggcgcaga ccccgagcgc agccagcacc
      661 aaaccacatt ggccgcaagt tcgcgccacg attcccgcag tgcctctcgg caagtagaga
      721 gcagaacttg aggaaaattt cccgcggcga gtaagtatca agcggacccc tccacgtcaa
      781 gagaatcacc gcttgcttgc attgctgcgc tgctggcaaa gatttcgcta gcgtcgcccc
      841 cgcgaatccc gacaatgacc ggtggcgccc ggccgtgttc aaccgacctc cggctacacc
      901 gcaagagcga tgacccccga tccgaacaat ccttattcgc cgccgcaagc cgagcccgcg
      961 cgtcccaagc cggactacga attcggcccc atgacccgcg tccgcttcct ggtcgtcggc
     1021 atgtgcgtcc tcatgtcggt cctgctctat atcgatcgct tcgcgaccac gccgaccacc
     1081 gacacgatcc tctccgagct aaagctctcc aaaaccgaat tcggcgacgc cattggggcc
     1141 ttctttctgg cctatgcctt gatgcaaatc cccagcggct ggatcaccga tagtttcggc
     1201 gctcgctgga tgctggccct atatgtcttg tgctggtcgc tggcaacgat cggactcggc
     1261 ttcgctcaag gattggcggc ggtttggacg atgcggctcg tcctcggcat catgcaagcc
     1321 ggggcctacc cgaccgccgc cggcctgctc aagcggtgga ttccctatac cgagcgcggc
     1381 ctggcgaaca gcatcgtctc gatgggcggc cgctgcggcc tcctcctgtc gctcgtgctc
     1441 accgtgccgc tgatgcgggc ggtgggcggc gtcaccggct ggcaactggg caattggcga
     1501 gcggtgtttg cgttctacgg cggcttgggc ctggcttggg cagccgcttt cgtctggctc
     1561 tttcgcgatt ggccgcgcga ccatccccgc tgcagtccag ccgaacgccg ctggatttcc
     1621 ggcgacgccg ccgaggccaa ggtcaaggac gaccccggct cgcaggggcg cgtctggcgg
     1681 ctcgggctct tctatgcgct gctcgtgggc gcgattctgg gcctgattct cgtgctcgtc
     1741 gccgcacgcg acctgctcat cgcacaattc ggtccttccc tcacccagct caccggcagt
     1801 gaagcgggca tgggtgcgat cgtcaatacg gtgccggagc tcggcgggct gatcggcacg
     1861 ctcgccctgt gcttcggagc gaacctgctg ctcgatcgct tctttccggc cgatcggcat
     1921 ctgcaattgc ccttccgcgc gatggcggca agcaaggaag tctggctgat gtgtgccatc
     1981 aatttcttcg tcaatattgg ctggatcctg ctcgcgacct ggctgccgca atacttgatt
     2041 gagaatcacg gcagcaccct cacggcgaca atcggcgaca aggtgttcgt agcggccttg
     2101 atcaccgccg tcgtgcaggt cgcggcgatg tgcggcggcc tgtccggcgg ccgggcgacc
     2161 gacgtcttcg tccgccggtt tggtcccacc tggggccgcc gcctgccggg catgtgcgcc
     2221 ggattgctcg tctgcggcat gtacctcgtc gtgccgcagc tcgcggacct gtggctgttc
     2281 gtggccatga tgatcgtcat cgcgtacacc atcgacttcg gcctcggcgc gacctgggcc
     2341 agctatcagg acatcggcgg ccgcaacgtc gccgccatcc tcggcgtcgg aaacatgtgc
     2401 ggcaatctcg gcgcggccta cttcggccgg ctgatcggca ttctggccga ccacaaccag
     2461 tggaacgccg tgttctacat cgccgccggc gccatgctcg ccgccgccag ctgctggatg
     2521 ctcttcgacg cctctcgcgc cgtcgtccaa gagcggtagg acggaccccg cgctcgtctc
     2581 gctccgcgag acggccgctg gcgactcgcc agcgcgcaac tcgccttgtt cccaggctcc
     2641 tgctcctgct cctgattcct gcacggggac gtacgtcctc gaggctccag cctcgcggct
     2701 atcccgcggt atcagctgcc gcggcggaac actgaagaac caggcagcat gcgatccgtc
     2761 ccaacgtctc cctgtcgctc gctctctccg tctccctctc cacaattagc cgcccagtgt
     2821 gaaaggttct tatccggacg gcggacgaaa aaaccctaaa aagcatcaga cgtagcggca
     2881 gtttatcttt tcattctgcc tcgatgagcc gtacaaaacc aacatcgata tactgcccgc
     2941 cgccggtttg gtggcagtgg accgcgagcg tgttggcgcc gtcttggagg gtggcggcga
     3001 gcgtcttttc gttcaggggg acgagcgtgt agtcggtgac gaagcctttc accgcggcga
     3061 tctgtttgcc gttgagatag acctcggtgt cttcgtcgtg gtggatcagg agggcgaggc
     3121 cgtcgagctt gtttccgggt agttcaacgc ttcgacgcag ccagatgtcg gcggtcttcc
     3181 actcggtgcg gaccttgctg ccaggggtgg tcggctcgcc gaagccgccc tggccttcct
     3241 tccaggaagc gtcgtcgaaa tcgggccgct cccagccctc ggcgggcttg gcagtagtat
     3301 agcgccacgt ctggggctca tcgcgcgagg tggggacaac gacgacctcc ttgggcggcg
     3361 gctggtggag tttctggtgc agttcggcga cccgctcggc gtcgaacttg atcacctcgc
     3421 ggtcgtacgt catgaagccg ttgacttcga tttcaacatc cgtcgtttgc gtgtaaatgc
     3481 cggccgcgag cccctggccg atgagcgggc ggagcttgaa caggagctgc tcgtaggccg
     3541 aattcagctc ctcgcgagtc ttgaagctgc ggtagcccca gttattcttg tcctgccagg
     3601 tgtgcccttc cagcggcagg cccaggccgc cgtattcgcc gaggaccgcg gcccgcgaca
     3661 cttcgagctt gggaatcgtc ggccccggat agcggtgcag atcgacgatg tggccgaccg
     3721 gaaaatcgtt cccgccgctg gcgcagttca ccaggcgcgt ggggtcgagc tcttgggtga
     3781 actttgtgat gcccgccgtg tcgaactgcc cccacgcctc gttgaacggc acccagacga
     3841 cgatgcacgg gaaatggctt aagtctttga caatttcgct ccattcgttc ttgaaattct
     3901 cagccgattc tttcgagcgc tggatttcct cgccgttgtg ttcgccggtc ggccgccacg
     3961 gggcgtgctt gtcgccgctg ggcatatctt gccaaacgag caggcccagc ttgtcgcagt
     4021 gatggtacca gcgggccggc tcaacct
//
LOCUS       DASUGB010000855         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_788811, whole genome shotgun sequence.
ACCESSION   DASUGB010000855 DASUGB010000000
VERSION     DASUGB010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788811"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1727)
                     /locus_tag="VFV87_16215"
     CDS             complement(393..1727)
                     /locus_tag="VFV87_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5105365.1"
                     /translation="MTTRLSRRTVLKGAGCAIGLPLLEAMLPTRGIAAEVAKPPVRMG
                     FVSFPNGAIMDAWRPKRLENGNFQLGETLQPLEDVFERVTVIAGLAQDNGRAKGDGAG
                     DHARSAASLLTGAHPVKTSGSNIRVGQSVDQAAAERIGHLTRLPSLEIGLERGRDAGG
                     CDSGYSCAYSNNISWKNANTPMSKEINPRLVFERMFGNKQDAQTLARRTRVRQSVLDL
                     VAEQAADLKNRLGTTDQQKVDQYFTSVREIEQRISRAEQSGQVERPDFETPEGVPGDV
                     VEHMRLMYDLMTLAFQTDATRIVTYMLANEGSNRSYKMVGVNDGHHEISHHRNEKDKV
                     EKLKKIDLFLATEFARFVRKLQSIPEGDGTLLDNCMIMYASGLSDGNRHDHDDLPIVL
                     AGRAGGTIDSGRFIDLDREIPLNNLFLSMLDRVGAGIPSLGDSSGRLTTIDA"
     gene            complement(1724..4027)
                     /locus_tag="VFV87_16220"
     CDS             complement(1724..4027)
                     /locus_tag="VFV87_16220"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5105366.1"
                     /translation="MAILTAVATCAPAAAQTPKPPANDQAFQRDVVPFLTKHCLDCHS
                     GKEPEGEVSLDVFKGPASIAADQKTWQRVIEMLRSGAMPPEDAEQPPMPARRHMVSWI
                     EKTIYNVNCDVPDPGHVTIRRLNRAEYNNTVRDLLGITFKPADDFPSDDVGSGFDNIG
                     DVLTVPPILIEKYLTAAERIAEEAIIADPQQFVKAQRHDRRELSGSGSADYDDERRRW
                     TISSEDGAVSAEFDFPREGRYRIRATASQQAAGDEAAKMELRLGSQKLKEFVIDTFRE
                     PKTCEITTTAKAGKQRLSAHFLNDFYDPDNPNERRRDRNLRVYAFEVDGPLDVGPEDY
                     PEIHRKLINVRPGDKLSVLDAAKTNLRPLIDRAFRRPATDEEVERFAGLVKNAVEQGD
                     SFEQGMQVGLIGVLVSPHFLFRAENDKSNAVSDFELASRLSYFLWSSMPDDELFALAA
                     QKKLQDPAVIEQQVRRMLADPKSEALVQNFATQWLNLRLLDAITPDPRVYPDFDAALK
                     ADMRRETELFARAVIQEDRSILDFLDGRFTYVNERLAKHYGIPGIEGDEFLRVSFADK
                     DRRSGVLTQASILTLTSNPARTSPVKRGKWILENILGTPPPAPPMDVPDLEETAKARP
                     GASLREQLEIHRSNAVCASCHKTMDQLGFGLENFDAVGRWRDKDGRHAIDASGELPGG
                     SKFNGPLELAGVLKARQTEFAQCLAEKMLTFALGRELRVQDRCTVDKIVEEVERRDYR
                     FSALAIAIATSDPFRKRSAQASQGTQP"
BASE COUNT          743 a         1325 c         1402 g          893 t
ORIGIN      
        1 ccgcgggggc cgagcgtgct gcggacggcc cgggcgagct tcgacacgcc ggccaagagc
       61 ggctggcggc agtcgtcgtc aaagaccatt tgctttgcca cgttgagaat cctcctaatg
      121 cggatggacg attgagtgtg cggtgggaag ggcccgggtt ggctgtgttc tgccgctgcg
      181 gccgaacaca ggaggccaca gcttcagccg gtctagtcct tcagcgacca aactggcagt
      241 ctgggaatgt cgcccggcag aaatgcaagc gctgtgccaa gcccacaatg atcaccgcaa
      301 gccgttgcat agcaagatgt tacgcagtgc agcgtcgcta tcgcgatgcg cttggcattc
      361 gggattgcca atatggcaga ccccaaatca cgctacgcat caatcgtcgt cagccttcca
      421 ctgctgtcgc cgagcgaggg gatgccggcg ccgacgcggt cgagcatcga gaggaacagg
      481 ttgttgagcg gtatttcgcg gtccaggtcg atgaagcggc cggaatcgat cgtgccgccc
      541 gcccggccgg cgaggacgat cggcaggtcg tcgtgatcgt gacggtttcc gtcggagagg
      601 ccgctggcgt acatgatcat gcagttatcg agcagcgtgc catcgccctc gggaatcgat
      661 tggagcttcc ggacgaagcg ggcgaactcc gtcgccagga acaaatcgat ctttttgagc
      721 ttttctacct tgtccttttc attgcggtgg tgcgaaatct cgtggtggcc gtcgttcacg
      781 cccaccatct tgtagctgcg gttgctacct tcgttcgcca gcatgtacgt gacaattcgg
      841 gttgcatcgg tctgaaaggc cagcgtcatc aggtcgtaca tcagccgcat gtgctcgacc
      901 acatccccgg gcacgccctc gggcgtctcg aaatcgggcc gctcgacctg gcccgactgc
      961 tcggcccggc tgatgcgctg ctcgatctcg cggacgctcg tgaaatactg atcgaccttc
     1021 tgctggtcgg tcgtgcccag gcggttcttc aggtcggcgg cctgctcggc aaccaggtcc
     1081 agcacgcttt gccgcacgcg ggtgcggcgc gcgagcgtct gggcgtcctg cttgttgccg
     1141 aacatccgct cgaaaaccag ccgcgggttg atctccttgg acatcggcgt gttggcgttc
     1201 ttccaggaga tgttgtttga gtaggcgcag ctatagccgc tgtcgcagcc gccggcgtcg
     1261 cggccgcgtt ccaggccgat ttcgagcgac ggcagccgcg tgaggtggcc gatgcgctca
     1321 gcggccgctt gatcaacgct ttggccgacg cggatgttcg acccggaagt cttcaccgga
     1381 tgggccccgg tcaaaagcga tgcggcgctg cgggcgtggt cgccggcgcc gtcccccttg
     1441 gctcggccat tatcctgagc caggccggcg atgaccgtaa ctcgctcgaa aacgtcctcc
     1501 agaggctgca gcgtctcgcc gagctggaaa ttgccattct ccagccgctt cggcctccag
     1561 gcgtccataa tcgccccgtt cgggaacgag acgaagccca tccgcaccgg cggcttggcg
     1621 acctcggcgg cgatgccacg agtgggaagc atggcttcga gcaggggcag accaatggcg
     1681 catcctgcgc ccttaagaac ggtgcggcgg ctcagtctgg tggtcatggt tgggttcctt
     1741 gagatgcctg agcggatcgc ttgcggaatg gatcgctggt cgcaatggct atcgccagtg
     1801 cagagaagcg ataatcccgc ctttcgacct cctctacgat tttatcgacc gtgcagcgat
     1861 cttgcacgcg aagttcgcgg cccagggcga acgtgagcat tttttcggcc aggcactggg
     1921 caaattccgt ttgccgggct ttcagcacgc cggccagttc cagcggtccg ttgaacttac
     1981 tgccgccggg cagttcgccc gaagcgtcga tcgcgtgccg gccgtccttg tctcgccagc
     2041 gaccgaccgc gtcaaagttc tccagcccaa agccgagctg atccatcgtc ttgtggcagg
     2101 aggcacagac ggcattgctg cggtgaatct caagttgctc gcggagcgaa gcgcccgggc
     2161 gagccttggc ggtctcttcc aggtctggca catccatcgg cggcgccggg ggcggcgtgc
     2221 cgaggatgtt ctccagaatc cacttgccac gcttgacagg cgaagtgcgg gccgggttgc
     2281 tggtgagcgt gagaatgctt gcctgcgtca gcacgccgct gcgacggtct ttgtctgcaa
     2341 aggaaacgcg gaggaactcg tccccctcaa ttcccggaat gccatagtgc ttcgcgagcc
     2401 gctcgttgac gtatgtgaag cggccgtcga ggaaatcgag gatgctgcga tcttcttgaa
     2461 tgaccgcccg cgcgaagagc tccgtttcac ggcgcatgtc ggctttgagg gcggcgtcga
     2521 aatcgggata aacgcgggga tcgggcgtga ttgcgtccaa tagccgcaag ttgagccact
     2581 gcgtggcgaa attctggacg agggcctcgc tcttggggtc ggcgagcatc cggcggactt
     2641 gctgctcgat gaccgccgga tcctgcagct ttttctgcgc ggccaaggcg aacaactcgt
     2701 cgtccggcat gctgctccag aggaaatacg aaagccgcga ggcgagctca aagtcgctta
     2761 ccgcgttgct tttgtcgttt tccgcacgga atagaaaatg cggcgaaaca agcacgccga
     2821 tcagcccgac ttgcatcccc tgctcgaacg aatcgccctg ctcgacggca tttttaacga
     2881 gcccggcgaa acgctccact tcttcatccg tcgccgggcg gcggaaggcc cggtcgatca
     2941 gcggccgcag gttcgtcttg gccgcgtcga gcacggagag tttgtcgccc ggccgcacgt
     3001 tgatcaattt gcggtgaatc tcgggataat cctccggccc gacatcgagc ggtccatcga
     3061 cctcaaaggc gtacacacgc agattgcggt cgcgccgacg ctcgttgggg ttgtccgggt
     3121 cgtagaaatc gttgaggaaa tgggcgctga gccgctgctt gccggctttt gcagtagtgg
     3181 tgatctcgca cgtttttggc tcgcggaaag tgtcgatcac gaattccttg agcttttgac
     3241 tgccgagccg cagttccatc tttgcggctt cgtcgccggc ggcttgctgg ctggcggtgg
     3301 cgcggatgcg gtaccggccc tcgcggggga aatcgaattc ggcggaaacg gcgccgtctt
     3361 cgctggaaat cgtccagcgg cggcgctcgt cgtcgtaatc ggccgagccg ctgccgctca
     3421 gctcgcggcg gtcatgccgc tgggctttca cgaattgctg cgggtcggcg atgatcgcct
     3481 cttcggcgat ccgctccgca gcagtgaggt acttctcgat caggatcgga gggacggtca
     3541 gcacgtcacc gatgttgtcg aacccgctgc cgacgtcgtc ggacggaaaa tcatcagccg
     3601 gcttgaacgt gatgcccaag aggtcgcgga ccgtgttgtt gtattccgcg cggttgagcc
     3661 ggcggatcgt aacgtggccg ggatcgggca catcgcaatt gacgttgtag atcgtcttct
     3721 caatccagga gaccatgtgc cgccgggcgg gcattggcgg ttgctcggca tcttcgggag
     3781 gcattgcacc cgagcggagc atctcgatca cgcgctgcca ggttttctga tcggctgcga
     3841 tcgacgcggg acctttgaag acgtcgagcg agacctcgcc ttcgggctct ttgcccgaat
     3901 ggcaatcgag gcagtgcttc gtcaggaacg gaacgacgtc ccgctggaag gcttggtcgt
     3961 tcgctggagg tttaggcgtc tgggcggcgg caggcgcgca cgttgctacg gcggtgagaa
     4021 ttgccaccca atagatgcac gttgcccgcg gtgctgttcg cacttcggtc ccccaaatca
     4081 gcgctcaatc cgccttcaat ctcgttgcaa catctattta accatcttga tgcatcggcg
     4141 ttacgcggca agcggttatt tgcaggcaat ctccgcaagt cgttgcagtc aacggcttta
     4201 cgcagcaacg attcaggccg ttcgatgctg ccgctcggcc tccgccagca ccaggagcat
     4261 cttctggtgg acgaccgggt tggccgatgc cagaaactgg ggcgattcca aggcaactgg
     4321 ctggccgctg atggccgtca ccacgccgcc cgcctcttcc acc
//
LOCUS       DASUGB010000856         9591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_234268, whole genome shotgun sequence.
ACCESSION   DASUGB010000856 DASUGB010000000
VERSION     DASUGB010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234268"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFV87_16225"
     CDS             complement(<1..589)
                     /locus_tag="VFV87_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5105367.1"
                     /translation="MASTFLSKLQDMFKDRRVDIAERFQIVREAVSGTMSNFFMATDR
                     TTGKTVGLKLLDPEKCAAFEARFKGLKKPSEGEIALKLKHPRIVETLEYGKTKDGKQY
                     ILMEFVEGTGLHTLIKSRDLLLGNNRALLFRQMAEAIEAVHKAGFIHRDICPRNFVVD
                     PEAKTMKLIDFGLTLPATAEFMRPGNRTGTPAYMSP"
     gene            complement(769..1902)
                     /locus_tag="VFV87_16230"
     CDS             complement(769..1902)
                     /locus_tag="VFV87_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03009 domain-containing protein"
                     /protein_id="HEX5105368.1"
                     /translation="MTRRLISYALLVTLAVAGTALAQTNPGSAVPSRYLPPAGGQVQS
                     RPAQPRPAQPYTGPQPIRQVGGQEPIRQPMGPLNAVVPAAPAGPLAPEWFPLAPAQQQ
                     WVDQILAFWEQESSQVKTFECKFQRWEYDPVFGSKTDAKTYAEGVIKYAQPDKGLYRV
                     EKLSSYSPPAKPGDKPQYIEQDATFGEHWVCDGKQVFTLNARNKQMVVTPLPPEMQGK
                     AIVDGPLPFLFGAKAETIKARYWIRVLPETGNGKFWLEAVPKGRQDAQNFKMIHIVID
                     EQRFLPEMLQLFAPNYDPTTNPSRTTHVFKDVKTADANAIQAAFVKGVNHLNIFHREF
                     YEPGLPSGWKKVVESDVAVSTPVPQEAKGPADVPKRTFLPLPR"
     gene            complement(1974..2567)
                     /locus_tag="VFV87_16235"
     CDS             complement(1974..2567)
                     /locus_tag="VFV87_16235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5105369.1"
                     /translation="MISGIVFDLDGTLVDSGLDFEAMRREMELPPGEPILEALKHLQP
                     AHAARSGEVLHRHEREGLARATLLPGVRELLATLKRRRIHTAIATRNSRSITAATLAR
                     FQLTFDPVLTRDDGPVKPDPWPVLHACQQWGLGPSQVVVVGDYRFDVESGRAAGARTV
                     LLTHPQDPRQYANHEQADLLLTSLAEYPRLLAWLEAL"
     gene            complement(2564..3613)
                     /locus_tag="VFV87_16240"
     CDS             complement(2564..3613)
                     /locus_tag="VFV87_16240"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5105370.1"
                     /translation="MSSASITDAATGPAPWRLRGACRTLAGGWQTFFHAPCDARVAAA
                     VRIAFAMVMLLGLAILYPDLDLWFTDAGVLPQDASREVAPPQTWSIFWQVPATEAAVR
                     ICFWIAVTQAVCLLLGLASNLNMLCLFVWLMSIENRNFLIIDGEDRVLRMLAFMLIWA
                     PLGRCWSVDSLVRRWWKGSGVNCGQELSTLQERGPQLTEDPLVSVASRYAAPGWGLRL
                     VQIEICLVFFSAGLCKLGGDAWIDGTALYYVARLDDYFGRFPTPAWPFDSPWAVAAMT
                     WSVIAVELLAPVFIWFKETRRITLAVVVLFHLANEWTMHLFLFHWVMLTGWLAFVSPS
                     DFAWLGRLFRKSKSA"
     gene            complement(3610..4140)
                     /locus_tag="VFV87_16245"
     CDS             complement(3610..4140)
                     /locus_tag="VFV87_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105371.1"
                     /translation="MLSRLKLWAINAFIATLLALMLIDTLPQSPIALRLAIQPVLRRT
                     GLHQGPWNMFTPNPDAINLKLRAEVTYRDGQKAQWESPDWRSQPNWERWVGHRQQEFC
                     DMIATQEAAPAWRTWAQFLARTLRPDFPNAERGAEVRITYQEAPVPPAEKQPWTTWRE
                     PPPYGDTWFLTIEKFP"
     gene            complement(4224..4439)
                     /locus_tag="VFV87_16250"
     CDS             complement(4224..4439)
                     /locus_tag="VFV87_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105372.1"
                     /translation="MPSIANRNHSSASSAAPQTTVYYLVWRISPSQPWRSEEFFNRID
                     AHNRYFALLQRGVEAYLERRQSAVLSA"
     gene            4626..5966
                     /locus_tag="VFV87_16255"
     CDS             4626..5966
                     /locus_tag="VFV87_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5105373.1"
                     /translation="MKTLIRDATAVLPEGSVRINVLIEGGTIAAIDPAIHTPADEVVS
                     ARGLHLLPGVIDDQVHFREPGLTHKEDLAAATRACAKGGVTTFLEMPNTIPTTTTVER
                     LHEKLALAATKCLVNYGFYMGATLTNVAELKLAMRTPGIKIFIGSSTGEMLVDDQAAL
                     ETIFAETTLPICAHCEDESTIRANAERLKDTTNVADHSRVRDHAAAIIATRRAIDLAF
                     RHKHRFHVLHVSTGDEADLLADHRNLITAEVCPHHLFFNTDDYARLGTLVQMNPSLKT
                     ADDNRRLWQALLDGRIQVIATDHAPHTLEEKRKPYSPGHGGSPSGLPAVENSLALLLN
                     EVHRGRCTLEQVARWMCEAPARVWDIVNKGRIQVGYDADLVLVDLNKSATILNEQQET
                     KSRWSPWHGVTLTGWPVRTWVLGQEVFRDGQINASLRGREAQFDHTRGGYWVTS"
     gene            complement(5976..6614)
                     /locus_tag="VFV87_16260"
     CDS             complement(5976..6614)
                     /locus_tag="VFV87_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105374.1"
                     /translation="MLRRTFAFALAGLLVAIPALAQEEAAQEEAKEFSGPQKGEAITP
                     FKIRGLLGDKAGEEYDLVKEAKGQPLVLVFVHEVNRPSVGLARVVMTYAASRKADGLH
                     AGLIFLTADATATEEWVKRASGALPQGVPVGLSPDGAEGPGAYGLNRKVTMTVLVAKD
                     DKVTANFALVQPSIAADGPKIIEAIVGVAGGKAPTAEELQKLTAPMRPARAP"
     gene            complement(6826..7239)
                     /gene="rpsI"
                     /locus_tag="VFV87_16265"
     CDS             complement(6826..7239)
                     /gene="rpsI"
                     /locus_tag="VFV87_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5105375.1"
                     /translation="MVAVKKDKSTGDSLGTGRRKKAIARVRVRPGSGKITVNGDDLPV
                     YFPIIAQQQSVLDALDATAHREKVDLIITCSGGGKHGQAVACKMGIARALIGFDGETF
                     ATLRDGGFLTRDSRMKERKHYGLRGARRGVQFSKR"
     gene            complement(7399..7842)
                     /gene="rplM"
                     /locus_tag="VFV87_16270"
     CDS             complement(7399..7842)
                     /gene="rplM"
                     /locus_tag="VFV87_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5105376.1"
                     /translation="MAGTKTYVAKPGEVAEKWWVVDASDKVVGRLASDIAVILMGKHR
                     PQYTPHVLCGDSVIVINAEKVHFTGKKWAQKEYAWFTGYTGLRKETAERRLSRHPDRI
                     LRDAVRRMLPKNKLATKMLQRLKVYPGADHPHQAQEPQPLELGKK"
     gene            8177..8929
                     /locus_tag="VFV87_16275"
     CDS             8177..8929
                     /locus_tag="VFV87_16275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105377.1"
                     /translation="MRHLNIPTRPGNLLLAAALLVIASGCRMVSTGRNVDGVRYFQQG
                     QYPVALQRFDAAMSIDPRNPDSYYNKGAIYHKMGLAERNQNLLTQAESLYQQCLAYNP
                     DHVDAHRGLAVLLTETGRVPQSFDLLKSWVARSPQNADARVELARLYEEYGDRQTAEQ
                     ALNDALAVNMQNWRAHAALGRLREQSGDLAQALQNYERSYTLNHFQPQLEQRIADLRG
                     KVSPLAPGTTTPLPGTTIATRNAAAPAPFRRY"
     gene            complement(8945..9292)
                     /locus_tag="VFV87_16280"
     CDS             complement(8945..9292)
                     /locus_tag="VFV87_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105378.1"
                     /translation="MTEVVIRRTGKGGSGIVIAVIMLAVLIFVRYGPQLAKQDARQRE
                     NLERQRLQQHILETTTSEELSRLRKSLEESDAVEKSLERLHNAAEARSQAEPEEDSTP
                     SDVPDSEVGSPSR"
BASE COUNT         1763 a         3133 c         2993 g         1702 t
ORIGIN      
        1 cgggcgacat gtaagccggt gtcccagtgc gattacccgg ccgcatgaac tccgccgtcg
       61 ccggcagcgt caacccaaag tcgatcagct tcatcgtttt cgcttcaggg tcgaccacga
      121 agttccgcgg gcaaatatcg cggtggatga agcccgcctt gtggacggcc tcgattgcct
      181 cagccatctg gcggaacaat agagcgcggt tgttgcctag caacaagtcg cggctcttga
      241 tcagcgtatg gaggcccgtc ccctcgacaa attccatcag gatgtactgc ttgccgtcct
      301 tggttttgcc gtactccagg gtttcgacga tccgcggatg cttgagcttg agggcgatct
      361 cgccttcact cggcttcttg agccccttga agcgggcctc gaaggcggcg cacttttccg
      421 gatcgagcag tttcaggccc accgttttgc cggtcgtgcg gtcggtggcc atgaaaaagt
      481 tcgacatcgt cccgctgacc gcctcgcgca cgatctggaa ccgctcggcg atatcgacgc
      541 gccggtcctt gaacatgtcc tgcagcttgg acaggaaagt gctggccatg cgcggtcgct
      601 cagcgtggtt gttccggaga gaaaggcgcg cggccggcag aaccccaaaa ccagccgccc
      661 gcgcagccga ggcctcaagg gtcaactcta aattcgcgga agcggaaggg caagctggcg
      721 cggcgcgggg aattcatgga gcgggggaca ggggacagag gacaggggct aacgcgggag
      781 cggcaggaag gtgcgtttgg ggacgtcggc cgggcctttg gcttcctggg gaacgggcgt
      841 cgaaacagcc acatcgcttt cgaccacctt tttccagccg gagggcaatc cgggctcgta
      901 gaattcgcgg tggaagatat tcagatggtt cactcccttt acaaatgccg cctggatggc
      961 gttcgcatca gccgtcttca cgtccttaaa cacgtgcgtc gtccgcgagg gattcgttgt
     1021 tggatcgtag ttcggggcga acagttgtag catttccggc aggaaccgct gctcgtcgat
     1081 cacgatgtgg atcatcttga aattctgagc gtcctggcgc cccttcggca ccgcttcgag
     1141 ccagaacttg ccgttaccgg tttccggcag aacgcgaatc cagtagcgag ccttgatcgt
     1201 ttcggccttc gcgccgaaca gaaacggcag cgggccatcg acgatcgcct tgccttgcat
     1261 ctcgggcggc aggggagtca cgaccatctg cttgttgcgg gcgttgaggg taaagacctg
     1321 cttgccatcg cagacccaat gctcgccgaa ggtcgcgtcc tgctcgatat actgcggctt
     1381 gtcgcccggc ttcgccggag gtgaatacga cgagagcttc tccacgcggt acagcccttt
     1441 gtcgggctgg gcgtatttga tgacgccctc ggcataagtc ttggcatcgg ttttcgaacc
     1501 gaagaccggg tcatactccc accgctgaaa tttgcactca aacgtcttga cctggctgct
     1561 ctcttgctcc cagaaggcca ggatctgatc gacccactgt tgttgggcag gcgcaagcgg
     1621 gaaccactcc ggcgcaagcg ggccggcggg agcggcgggg acgaccgcat tgagcggccc
     1681 cattggctgg cggatcggct cctggccgcc aacctgccgg atcggctgcg ggccggtata
     1741 gggctgggct ggccgcggct gtgccggtcg cgactggact tgcccgccgg caggcggaag
     1801 atagcgcgag gggacggctg agccggggtt cgtttgggcc agcgcggtcc cagcaactgc
     1861 caacgttacg agcaaggcat acgaaatcag ccggcgggtc atccgtgacc tcctcgagat
     1921 gcaggactca agaatgaatg gcgcgggatc gtatcatccg tccccgccag cacctagagc
     1981 gcttcaagcc acgctagcag ccgcggatac tcagccagcg aggtcagcag cagatccgct
     2041 tgctcgtggt tggcatattg ccgcggatcc tgcggatgcg tcagcagcac agtcctggcg
     2101 ccagccgctc ggccgctttc aacatcgaac cggtaatcgc cgacaaccac gacctggctg
     2161 ggcccaaggc cccactgctg gcacgcgtgc agcacgggcc aaggatcggg tttcaccggg
     2221 ccgtcgtcgc gcgtcagcac cggatcgaac gtcaattgaa atctcgcgag cgtcgctgcg
     2281 gtaatcgacc ggctgttacg ggtggcgatg gcggtgtgaa ttctgcgccg cttgagcgtt
     2341 gccagcaact cgcgcacgcc aggcaacagc gtcgcccgtg ccaggccttc ccgctcgtgc
     2401 cggtgcagca cctcgccaga gcgcgccgcg tgggccggct gaagatgttt cagcgcctcc
     2461 aggatcggct cccccggcgg cagctccatc tcgcggcgca tggcctcaaa gtcgaggccc
     2521 gaatcgacga gcgtgccatc gagatcgaaa acgatgccgg aaatcacgcg ctcttggact
     2581 ttcgaaacag gcggccgagc caggcaaaat cgctggggct gacaaacgcc agccagccgg
     2641 tgagcatcac ccagtgaaac agaaacaggt gcatcgtcca ctcgttggca aggtggaaca
     2701 agaccaccac cgcgagcgtg atccgccgcg tttccttgaa ccagatgaag accggagcga
     2761 gcagttcgac cgcgatcacc gaccaggtca tcgcagcgac ggcccagggc gaatcgaacg
     2821 gccacgccgg cgtggggaag cggccgaaat agtcgtcgag ccgggccacg tagtacaggg
     2881 ccgtgccgtc gatccaggca tcgccgccga gcttgcacag cccggccgaa aaaaagacca
     2941 ggcaaatctc gatctgcacc agtcgcaggc cccagccggg agcggcatat cgcgacgcga
     3001 cggatacgag ggggtcctcc gtcaattgcg gtccacgctc ttgcaaagtg gacaactctt
     3061 gaccgcaatt gactcccgac cccttccacc agcgccgcac gagtgagtcc accgaccagc
     3121 atcgccccag cggcgcccag atcagcatga acgccagcat ccgcaggacc cggtcttcgc
     3181 cgtcgatgat cagaaaatta cggttctcga tcgacatgag ccagacgaac aggcacagca
     3241 tgttcaggtt ggacgccagg ccgagcagga ggcaaacggc ctgtgtgacc gcaatccaaa
     3301 agcaaatccg taccgccgct tccgtagccg gaacttgcca gaagatcgac cacgtttgtg
     3361 gcggggctac ctcgcgcgat gcgtcctgcg gcagcacgcc ggcgtcggtg aaccacaagt
     3421 caaggtcggg atacagaatc gccaggccca gcagcataac catcgcaaac gcgattcgca
     3481 cggccgcagc gacgcgcgcg tcgcaaggtg cgtgaaaaaa tgtctgccag ccgcctgcca
     3541 gcgtccggca cgcgccgcgc aagcgccacg gagccgggcc ggtggcagcg tcggtgatgg
     3601 aagcggagct catgggaact tttcgatcgt caggaaccac gtgtcgccat agggtggcgg
     3661 ctcgcgccac gtggtccagg gctgtttttc ggcgggcgga accggggcct cttgatacgt
     3721 gattcggact tccgctccgc gctcggcatt cggaaaatcg ggccgcagtg tgcgagccag
     3781 aaactgggcc cacgtccgcc acgccggcgc cgcctcctgc gtggcgatca tgtcgcagaa
     3841 ttcctgctgg cgatggccga cccaccgctc ccagttcggt tgcgaccgcc aatcgggcga
     3901 ctcccactga gctttctggc cgtcacggta agtcacttcc gctcgcagct tgagattgat
     3961 cgcgtcgggg ttcggcgtga acatattcca cggcccctgg tgcaatccgg tgcgccgcag
     4021 caccggctga atcgccagcc gcagcgcgat cggcgattgc ggcagcgtat cgatcagcat
     4081 cagcgcgagc agggtcgcga tgaacgcatt gatcgcccag agtttcaggc gggacagcat
     4141 gacgaacaac cagcggcggg attcggaccg ttccgctcaa gtgtaggtca tgccgcgcca
     4201 gccgcgaatc gcgatcgtgc cgtctatgcg gacagcacag ccgactgtcg ccgttccagg
     4261 tacgcctcca cgccccgctg cagcagcgcg aagtaacggt tatgggcgtc gatgcggttg
     4321 aaaaactctt cgctccgcca gggctggctg gggctgatgc gccagacgag gtaatagacc
     4381 gtcgtctgcg gcgcggcgga cgaggctgat gaatggttgc ggttggcgat cgagggcatc
     4441 ggcggaatct cgctgacaag aggcggttgg caatcgcttt ctgagcccgt aactctccag
     4501 cggaatcccg agccatttgt tacacccgtg gggcagacat tcctgtctgc cagcaaaaac
     4561 ccccgtcccg cgatagaatt cgagtcgctc ctgctccctg acccctgact cctgactcct
     4621 gccccgtgaa aaccctcatc cgcgacgcaa ccgccgtcct ccccgaaggt tccgtccgca
     4681 tcaacgtgct catcgaaggg ggcacgatcg cggccatcga cccggcaatc cacacgcctg
     4741 ccgacgaagt cgtctccgcc cgcgggctgc acctgttgcc cggcgtgatc gacgaccagg
     4801 tccatttccg cgagcccggc ctgacgcaca aggaggatct tgccgccgcc acgcgggcct
     4861 gtgccaaagg gggcgtgacg acgttcctgg agatgcccaa cacgatcccc acgacgacga
     4921 ctgttgagcg ccttcacgaa aagctcgctc tcgccgcaac caaatgcctg gtcaactacg
     4981 gcttctatat gggagcgacc ctcacaaacg tcgccgagtt gaaactggcc atgcgaaccc
     5041 ccggaatcaa aatcttcatc ggctccagca ccggcgagat gctcgtcgat gaccaggcgg
     5101 ccctggaaac gatcttcgcc gaaacgacgc tgccgatctg tgcccattgc gaggatgaat
     5161 cgaccatccg cgctaacgcc gagcggctga aagacacgac caacgtcgcg gaccacagcc
     5221 gggttcgcga tcacgccgcg gcgatcatcg ccacgcggcg ggcaatcgac ctcgctttcc
     5281 ggcacaagca ccgctttcac gtgctgcacg tttcgaccgg cgacgaagcc gatctgctgg
     5341 ccgaccaccg caacctgatc acggccgaag tctgcccgca ccacttgttc ttcaatacgg
     5401 acgattacgc gcggctcggc acgctcgtgc agatgaaccc ctcgctcaaa acggccgacg
     5461 acaaccgccg gctgtggcag gcgctcttgg atggccgcat ccaggtcatc gccaccgatc
     5521 atgccccgca cacgctcgaa gaaaaacgca aaccctattc tcccggccac ggcggctccc
     5581 cgtccggctt gccggccgtc gagaactcgc tggcgctgtt gctcaacgaa gtgcatcgcg
     5641 gccgctgcac gctggagcaa gttgcacgct ggatgtgcga ggccccggcc cgcgtgtggg
     5701 acatcgtcaa caaaggccgc atccaggtcg gctacgatgc cgacctcgtc ctcgtcgatc
     5761 tgaacaagtc cgcgaccatt ctgaacgagc agcaggaaac caaatcccgc tggtccccgt
     5821 ggcacggcgt cacgctcaca ggctggcccg ttcgcacctg ggtccttggc caggaagtct
     5881 tccgcgacgg ccagatcaac gccagcctcc gcggccgcga agcccaattc gaccacaccc
     5941 gcggcggcta ctgggtcact tcgtagcgct tcaatttacg gcgcacgagc aggacgcatg
     6001 ggagcggtga gcttttgcag ttcttcagcg gtgggagcct tgccgccggc aacgcccacg
     6061 atggcctcga taatcttcgg accgtctgcg gcgatgctgg gctggacgag ggcgaaattg
     6121 gccgtcactt tgtcgtcctt ggccacgagc acggtcatcg tcaccttgcg gttaagtccg
     6181 taggctcccg gcccttcggc cccgtcaggc gacaggccga ccggcacgcc ctgcggcagc
     6241 gcgccgctgg cccgcttgac ccattcttcg gtggcggtcg cgtcggctgt gaggaagatc
     6301 aggccggcgt gcaagccatc cgccttccgc gaagcggcgt aggtcatcac cacgcgggcc
     6361 agaccgaccg acgggcggtt gacctcatgc acgaagacga gcaccagcgg ctggcccttg
     6421 gcttccttta caaggtcata ttcctcgccg gccttgtcgc cgaggaggcc gcgaatcttg
     6481 aagggcgtta tcgcttcgcc cttttgcggg ccggagaatt ctttagcttc ttcctgggcg
     6541 gcttcttcct gggccagagc ggggatcgcg acgagcaagc cggcgagcgc gaaggcgaat
     6601 gtgcggcgga gcatgaggac ctccaggcgg tgaaaagggg ctcacccagc gatggaggcc
     6661 atcatagctg gtgggcggct gaattgctcc ttgtcgggat gtaggacagg ttttcaaccg
     6721 gtcctgttcg gcggacgggt tggaaacccg tcctacgatt ccgcgaccaa tggtcgtggc
     6781 tttgtggcag gcaatgggaa ccgcgaccga tggtcgcggc tttgcttagc gcttcgagaa
     6841 ctgcacgccg cggcgggcgc cgcgaaggcc gtagtgcttg cgttccttca tgcgcgaatc
     6901 gcgcgtcagg aagccgccgt cgcggagcgt ggcgaaggtc tcgccatcga agccgataag
     6961 ggcccgggcg atgcccatct tgcaggcgac ggcctggccg tgcttgccgc cgccggagca
     7021 ggtgatgatc agatcgacct tctcgcggtg ggcggtggcg tccagggcgt ccagcacgct
     7081 ctgctgctgg gcgatgatgg ggaaatagac gggcagatcg tcgccgttga cggtgatctt
     7141 gcccgagccg gggcggaccc gcacgcgggc gatcgccttc ttgcggcggc ccgtgccgag
     7201 ggaatcgccg gtggacttgt ctttcttgac tgcaaccatg gtgtcctctg aattcgctat
     7261 gttcgctatc gcattcgctg gttcccacgc tctgcgtgga aacccacgcc tggccgctcc
     7321 gcggcctgat cgcgacgcag agcgtcgatg cgtgcgttcc cacgcggagc gtgggaacga
     7381 ggggtgtgac cccctcgttt acttcttgcc caactccagc ggctgaggct cctgggcctg
     7441 atgcgggtgg tcggcgccgg gatagacctt cagccgctgc agcatcttgg tcgccagctt
     7501 gttcttggga agcatgcggc ggacggcgtc gcggaggatg cggtcggggt ggcgcgagag
     7561 ccgccgctcg gccgtttcct tccgcagccc cgtgtagccg gtgaaccagg cgtattcctt
     7621 ctgggcccac ttcttgccgg tgaaatggac cttttcggcg ttgataacga tgaccgaatc
     7681 gccgcacaga acgtggggcg tgtattgcgg gcggtgcttg cccatcagaa tcacggcgat
     7741 gtcgctggcc agccggccga cgaccttgtc gctagcatcg acgacccacc atttttcggc
     7801 gacttcgccg ggcttggcca cgtaggtttt cgttcccgcc acgttcgcat ctcccgctcc
     7861 cggcaggact tacatctcgg cacgcgcgac gcgccgattg ccgggcgaat ttgacaaaac
     7921 acgcaagata gccttctttt cgcgggcgta caagggtcgc agtggggcag acattctcgt
     7981 ctgccgcagt agccgcactc gccagagtgc gggaatccgc cgccggagcc gcattctggc
     8041 gaatgcggca gccaatcatg gcaggcaaga aagcctgccc cacttccgat cttgttgccc
     8101 gccaaaggga ttggtactgt tcgcggccct aaaaccaccc aatcccgccc cctccttgct
     8161 gggaagggaa tccgacgtga gacacctcaa cataccgacc cgacctggca acttgctgct
     8221 cgccgccgca ctgctggtca tcgcctccgg ctgccggatg gtttcgacgg ggcggaacgt
     8281 cgatggcgtg cggtatttcc agcaggggca gtatccggtc gccctgcagc ggttcgatgc
     8341 ggcgatgtcg atcgacccgc gaaaccccga cagctattac aacaaggggg cgatctatca
     8401 caagatgggc ctcgccgagc gcaaccaaaa cctgctgaca caagccgaat cgctctacca
     8461 gcagtgcctg gcctacaacc ccgatcacgt cgatgcccac cgcggcctgg cggtgctgct
     8521 gaccgaaacg ggccgcgtcc cgcaatcctt cgacctcttg aagagctggg tcgcccgcag
     8581 tccgcaaaac gccgacgccc gcgtggagct ggcccgcctg tacgaagaat atggcgatcg
     8641 acaaaccgcc gagcaggctc tcaacgacgc cctggccgtc aacatgcaga actggcgggc
     8701 ccacgccgcg ctcggccggc tgcgcgaaca atcgggcgac ctggcccagg ccctgcagaa
     8761 ctacgagcgg tcgtacacgc tgaaccattt ccagccgcag ctcgagcagc ggattgccga
     8821 tctccgcggg aaggttagcc cgctggcccc cggcactacg actccgctcc ctggcacgac
     8881 catcgccacg cgcaatgccg ccgccccggc ccccttccgg cgatactaac gccagagccg
     8941 agtcctatcg cgagggagag ccgacttccg agtctggcac gtcggaagga gtcgaatcct
     9001 cctccggctc cgcttgagat cgcgcttctg cagcgttgtg aagcctctcc agggacttct
     9061 ccacagcatc gctctcttcc agcgatttgc ggagccgact cagctcctcc gaagttgtgg
     9121 tttccagaat gtgttgttgt aggcgctgtc gctcgaggtt ttcgcgctgc cgcgcgtctt
     9181 gcttcgccaa ctggggtcca tagcgcacaa aaatcaggac cgcgagcatg atgaccgcga
     9241 tgacgatccc gctgcccccc ttaccagtcc ggcgaatgac gacttcggtc acgatgttgg
     9301 ctcctgaact ccgagccatc gtagtcgatg gcgacagcgc cgccaatgaa ctgacgctga
     9361 tgcgcgggtg cttccgtgcg cgatctgaac caacgcgccc tcatccggga agcgcagcgt
     9421 gcctcagccg cgaagcggcg tcagtcagta gccgtgggcg tgagcccacg aattgcggcc
     9481 cacacaaaaa tcgccagccc cgaagggggg gcacgaatgg atggcgagtt ggtgcgcccc
     9541 atgtcgcccc tgtggggctc gacgtgcgtg gcggttttcc ttccgtgggc t
//
LOCUS       DASUGB010000857         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1624121, whole genome shotgun sequence.
ACCESSION   DASUGB010000857 DASUGB010000000
VERSION     DASUGB010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624121"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1193
                     /locus_tag="VFV87_16285"
     CDS             192..1193
                     /locus_tag="VFV87_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5105379.1"
                     /translation="MATDALAVLSYYSLQPPLVRRLQPLGNAGGWSGSRLWRVWAADD
                     RQLCLRRHPESWRAPDRLRFIHRVLQHVHAGGLSVVPVPLVATSGATIIQHNGYLWEL
                     TPWMPGIADFHSHPTRERLRSALQTLARFHLLAVTDGVPPVDHNLAPALIVRSVLRDI
                     GRRADRLTSHLQPILGNELNARAPRLLELVRARGTEIGPAVLAAVDKPWFLQPSIRDI
                     WHDHVLFSGDEVTGIVDFGAMNVDTPLTDIARLVGSLVADDAAARNFALDAYSEIRPL
                     TDDDRQLIDLLDHSGTVLAGLNWLTWLYAERRDMGPIEPIIRRLHEILARLETMPVR"
     gene            complement(1138..1977)
                     /locus_tag="VFV87_16290"
     CDS             complement(1138..1977)
                     /locus_tag="VFV87_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105380.1"
                     /translation="MKPIRLLFAAAWLAATGLALARPVAAEEGAAEGFTPSDEFQGWI
                     SDLVREQLPQQYEKRKNWGHTTRTLDGISIQLDNGKLKTHRKYKQANDGTWQMYRVDL
                     VDPEEKFEIRVANLRELEDGRVGLRITAVASLQAFGRQTLWEHGIQIYSLSAEADARV
                     RLTAEAAIASRLDPTRFPPDVYLLPEVTAAKVEILEFKLRRISDLHGPLVRSLSHTVR
                     EELEAKLAEDNAKLVAKLNAAIDKRKEKLKLSAADLVTTKWGKFIAPASSPSEPESRA
                     AGE"
     gene            2187..2663
                     /locus_tag="VFV87_16295"
     CDS             2187..2663
                     /locus_tag="VFV87_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105381.1"
                     /translation="MPWMKHKTYAQRLKAMRPDTRVEKLTGFRARFGSGTIAIPAASE
                     VEEIMRKVPKGKLITTKGIQEIVARRHGATMGCPIITGVGIWLVSHVAYEQLKAGKKN
                     VVPFWRTIKNSGDIAPNCPGSRNEVKKLLQKDGHKVVVRGKRYRVIDYKQALAKLD"
     gene            2673..3701
                     /locus_tag="VFV87_16300"
     CDS             2673..3701
                     /locus_tag="VFV87_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5105382.1"
                     /translation="MVEAGIPLVGPYDALLLVSFGGPERPEDVLPFLENVLRGRNVPR
                     ERMLQVAEHYRHFGGVSPINAQNRALIAALKDDFVAAGLDLPIYFGNRNWHPLLPDTL
                     RDMRDRGVRRAIALFTSAFSSYSGCRQYRENIAAAQAEVGEGAPQVDRLRMFFNHPGF
                     IEAMIDRTREALLKIEPPRTPAAHLLFTAHSIPLAMAQNCKYQTHFREASRLVAEGVG
                     HAHWRLVYQSKSGPPGQPWLEPDVGDALAEIAAAGGPRDVVLVPIGFVSDHMEVLYDL
                     DEEAKLKAEQLGLNLVRAGTVGTHPRFVRMIRELVVERASGSPNRPALGTLGASHDVC
                     PPDCCKPR"
BASE COUNT          682 a         1256 c         1182 g          721 t
ORIGIN      
        1 gttagacagc cggacgacgg gagcgccggc cgcttgaggc gagccgcaaa ccgggtggga
       61 aacgagctcg tcaaatatac cccattctac cggcaatacc atcattgacg agtgttttac
      121 cggtcccgag ggttgtacca accgtccggc tgatcgcgcc tgccccgccc ttgcccggat
      181 acgattagat catggccacc gacgccctcg ccgtcctttc ttactactcg ctccagcccc
      241 cgctggttcg ccggctccag cccttgggca acgccggcgg ctggagcggc tcgcggctgt
      301 ggcgggtttg ggcagcagat gatcggcagt tgtgcctgcg gcggcatccc gagagttggc
      361 gagcacccga ccgcctgcgc ttcatccacc gcgtactgca gcacgtgcat gccggtggct
      421 tgtcggttgt acccgtcccg ctggtggcca catccggcgc gacgatcatc cagcacaatg
      481 gctacctctg ggagctgacg ccctggatgc caggaatcgc ggactttcat tcccatccga
      541 ctcgcgagcg actgcggtcc gccctgcaga cgctggcccg atttcacctc ctggccgtga
      601 ccgacggcgt cccgccggtg gatcacaatc tggctccggc tctcatcgtt cgcagcgtgc
      661 tccgtgacat cggccggcgg gcggaccggt tgacaagtca tctgcagccg atcctgggaa
      721 acgagctcaa tgcgcgggcg cctcgcctcc tggagctggt tcgtgcgcgg gggacggaaa
      781 tcggtcccgc cgtgcttgcg gctgtggaca aaccgtggtt cttgcaaccc tcgatccgcg
      841 acatctggca cgatcatgtc ttgttctccg gcgatgaagt caccggcatc gtcgattttg
      901 gagcgatgaa cgtcgatacg ccgctgacgg atatcgccag gttggttggc agtctcgttg
      961 ccgacgacgc agccgcccgc aacttcgcac tcgatgcgta ttcagaaatc cggcccctga
     1021 cagacgatga ccgccaactg atcgacctgc tcgaccactc cggaaccgtc ctcgccggcc
     1081 tcaactggct cacctggctt tatgccgagc gccgcgatat gggaccgatc gaaccgatca
     1141 ttcgccggct gcacgagatt ctggctcgct tggagacgat gccggtgcga taaacttgcc
     1201 ccacttcgtc gtcaccaaat ccgctgccga cagcttcagc ttctccttcc gcttgtcgat
     1261 cgcagcgttc agcttggcga ccagcttggc gttgtcctcg gccaacttgg cttccagctc
     1321 ttcacggacc gtgtggctca gcgagcgaac cagcggcccg tgcaggtcgc tgatccgccg
     1381 cagcttgaac tcgagaatct cgactttcgc cgccgtcact tccggcagca gatagacatc
     1441 gggcgggaaa cgcgtcgggt ccagccgcga ggcgatggcc gcctccgcgg tcagccgcac
     1501 gcgggcatcg gcttcggccg acaggctgta gatctgaatg ccgtgctccc acagcgtttg
     1561 ccggccgaag gcctgcaggc tggcgacggc ggtgatccgc aggccgacgc ggccgtcctc
     1621 caattccctc aggttggcca cacgaatctc gaatttctcc tccggatcga ccagatcaac
     1681 gcgatacatc tgccacgtgc cgtcgttggc ctgtttgtac ttgcggtgcg tcttcagctt
     1741 gccgttgtcc agttggatcg agatgccgtc gagcgtccgg gtcgtgtgcc cccagttctt
     1801 tcgcttttcg tactgctgcg gcaattgctc gcggacgagg tccgaaatcc agccctggaa
     1861 ctcatccgac ggcgtaaagc cttcggctgc gccttcctct gccgccacgg gccttgccaa
     1921 cgccaggcca gtggcggcca gccacgcagc ggcgaataac aggcgaatcg gcttcatttc
     1981 ccggccccgg caggcaaaaa gaccttggcg tccaccagga aggctacgaa actggtcccc
     2041 tccggccaag agcgaccgaa cgtggggcag gcattcttgc ctgcgaatat gtcgcatcta
     2101 ccgtaacctt ttccggcaga caagaatgtc tgccccacgc gggaccaatc tgttaatatg
     2161 aatacctccc ctggagagcc cctcgcatgc cctggatgaa gcacaagacc tacgcccagc
     2221 gactgaaggc gatgcgcccc gatacgcgcg tcgagaagct caccggcttt cgggcccggt
     2281 tcggcagcgg gacgatcgcg atcccagccg ccagcgaggt cgaggaaatc atgcggaaag
     2341 tccccaaggg gaagctgatc accacaaagg gcattcagga aatcgtcgct cgccgccacg
     2401 gagcaacgat gggctgcccg atcatcacgg gcgtcggcat ctggctcgtc tcgcacgtcg
     2461 catacgaaca attgaaggcg ggcaagaaga atgtcgtccc cttctggcgg acgatcaaaa
     2521 acagcggcga tatcgccccc aactgccccg gctcgcgaaa cgaagtgaaa aaactgctgc
     2581 agaaggatgg acacaaagtc gtcgtccgcg gaaagcggta tcgggttatc gactataagc
     2641 aggcattggc caagctcgat tgaatcccca tcttggtcga ggctggaatt cccttggttg
     2701 gtccgtacga cgccttgttg ctcgtctcgt tcggcggtcc ggagaggccg gaggacgtgc
     2761 tgccgttctt ggagaacgtc ttgcgggggc gaaatgtgcc gcgggagcgg atgctgcaag
     2821 tggccgagca ttatcggcac ttcggcggcg tcagcccaat caacgcgcag aaccgggcgc
     2881 tgattgcggc gcttaaggac gatttcgtgg cagcggggct ggatcttccg atctacttcg
     2941 gcaatcgcaa ttggcatccg ctcctgccgg atacgctgcg cgacatgcgc gatcgcggcg
     3001 tgcggcgggc gatcgccctt ttcacttccg cgttcagcag ctattcgggc tgccggcagt
     3061 atcgcgagaa catcgccgcg gctcaggcgg aggtgggcga aggggcgccg caggtcgatc
     3121 ggctgcggat gttcttcaac cacccgggtt ttatcgaggc aatgatcgac cggacgcgtg
     3181 aggccctgct caagatcgag ccgccgcgaa cgcccgcagc ccatctgctc ttcacagccc
     3241 acagcattcc gctggcgatg gcccagaatt gcaagtacca gacgcatttt cgcgaggcct
     3301 cgcggctggt cgccgagggg gtcggccacg cccactggcg gctcgtctat caaagcaaga
     3361 gcggcccgcc gggccagccc tggctggagc cggacgtggg cgatgccctg gccgaaatcg
     3421 cagccgcagg cggcccgcgg gatgttgtgc tcgtgccgat cggcttcgtt tcggaccata
     3481 tggaagtact gtacgatctg gatgaagagg cgaagctcaa ggctgagcag ctcgggctga
     3541 acctggtgcg ggccggcacg gttggcacgc atccgaggtt cgtgcgaatg atccgcgagc
     3601 tggttgtcga gcgggcgagc ggttcgccga atcgccccgc gctgggcacg ctcggcgcca
     3661 gtcacgatgt ttgcccgccc gattgctgca aacccaggta gccgcactcg ccagcgcgag
     3721 ggcacccccg tgcaagattc ccgcgttctg gcgaacgcgg ctacgacaga tatatgccgt
     3781 ccacacccga atttctcgcc gactatcacg ccctgaccgc cggctgcggg ttcttggagc
     3841 t
//
LOCUS       DASUGB010000858         5607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1624716, whole genome shotgun sequence.
ACCESSION   DASUGB010000858 DASUGB010000000
VERSION     DASUGB010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624716"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1054)
                     /locus_tag="VFV87_16305"
     CDS             complement(197..1054)
                     /locus_tag="VFV87_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5105383.1"
                     /translation="MKLAKYQLPTGQHGVARVEGDQLIPLDLSGGQYQSLFEILDSDN
                     PNEAAEFLTKSKEKIPLAQAQLLPPIDRQEVWAAGVTYKRSQQARMEESETSASCYDR
                     VYASPRPELFFKASPHRVSGHGQPLRIRADSKWNVPEPELTLVLSSKLDLVGFTVGND
                     MSSRDIEGENPLYLPQAKCYNQSCGLGPWITLPVGMPPRSEIGIRLVIHRGGKIIYDG
                     RTSIDHMARTFEDLIGWLARDNSFPTGAFLLTGTGIVPETPFTLQPRDIVEIKIDGIG
                     TLLNPVVQG"
     gene            complement(1161..2231)
                     /locus_tag="VFV87_16310"
     CDS             complement(1161..2231)
                     /locus_tag="VFV87_16310"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5105384.1"
                     /translation="MLLIDGHLDLSMNAVEWNRDLTRPLAEVRQREAGKTDKVDRGKG
                     VCTFPEMRKGEIGLCIATQIGRYVAPGNALPGWHAPEIAWAQTQGQLAYYRAMEDAGQ
                     MVQIANKAALEKHIALWQNNAPADAPIGYVLSLEGADSIVNLSYLEKAWTGGLRAIGP
                     AHYGPGRYSPGTGAEGGLTAAGRELVKEMRRIGFVLDATHLTDDAFWEALEIFDGPVW
                     ASHNNCRALVPHQRQFSDEQLKELIRRDAVIGGAFDAWMMVPGWIRGKTTPTETGVKI
                     ATIADHIDHICQLAGTSRHCAIGSDLDGGYGTEQTPMDLDSIADLQKLTGIFETRGYS
                     PEDIAGIMHGNWIRKLSEALPA"
     gene            complement(2588..3619)
                     /locus_tag="VFV87_16315"
     CDS             complement(2588..3619)
                     /locus_tag="VFV87_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105385.1"
                     /translation="MNRFTGLALLCLLPSLGSIPAAEPIEVVDTDDEIKIATPTLEAT
                     IRTKGYVSGVAAGSLVDKKTGFRDAGFGLDIVDWIMEPGSDAAYRDKLPQEMVYQFGN
                     DYHGQRPKRSIEGPQICTKAKELSPEVIRGKDFVAITQSFQYQTAAPGKKTGSTWTQT
                     IVFPEGKRYFVSSDRIDAVNGSEAMFLRIDMPGHIKHKAGDSFSEVFLSYFDQPIPSK
                     EFLTNFAPDEKFNYRRDAWEEAGKKLPGRIIRGYRLRDPATGKEGPWLLGMTLDPAIV
                     SEAWCHQRGYVCMIEEFGERPIKAGEHFSAAFIVGYFDSIEDANKVYDQHRDHSGLTV
                     DATGWRLTK"
     gene            complement(3667..4323)
                     /locus_tag="VFV87_16320"
     CDS             complement(3667..4323)
                     /locus_tag="VFV87_16320"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX5105386.1"
                     /translation="MTPGWRAADAARFHYQRRKMTANAQGLIARIKERNADRKRATDG
                     GIHPRKPIAPVNAKALQAAERAIGFKLPELLRAIYLKVGNGGFGPGYGIMGIKGGARL
                     DGCTLEKCYANMIKLETENGTWRWPRQLLPLANYGCGMWSCVDCAYKKLPMILWDPNP
                     LDDDLEGAAARRNWAYSFWDQEKSLGTWLEGWLASEPEPEPKQPSKSWINKRLRLTIR
                     "
     gene            complement(4320..5510)
                     /locus_tag="VFV87_16325"
     CDS             complement(4320..5510)
                     /locus_tag="VFV87_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX5105387.1"
                     /translation="MSAATTSTARCAAGISTAENTSAALREAAALARESLGGAPDLAL
                     LFFSAHHVPDAERIAADARELLGTANVLGVTGESIVGTACEIEEGPALAVWLARWPGA
                     SISPMHLAFQRTPEGGALEGWPEHLAGEWPAGAFLIVMGDPFTFPADFLLERMNEDRP
                     GVPVIGGMASGGHEPGMNRLLFGDQVLTEGAVAVLVSGPVRVRTVVSQGCRPIGRPLV
                     ITKAERNVIYELGGRPALIQLREIFDELPTSEQRLVQRALHVGRVVSEYRDHFEQGDF
                     LVRNVVGIDSSSGAVAIGDYIRPGQTVQFHVRDQEAADAELKQLLSAAQKENAAPLGG
                     LLFTCNGRGTRMFSQPHHDAAAISRAFGPLPLAGFFAAGEIGPIGTQNFMHGFTASVA
                     LFVG"
BASE COUNT          933 a         1802 c         1789 g         1083 t
ORIGIN      
        1 accggggcga ggggagcccg attttttgcg catagagcga tcccgctggg tcttagcgtc
       61 tcaatctaac cgcgacttat tctaaaccga cgagaatcac ccgaattttc cgtgctccct
      121 tctccccggt actccgggga gaagggctgg ggatgagggg ttgccccagg gctgctaaag
      181 gaacgcatgt cagcccttac ccctgcacca ccgggttcag cagcgtgccg atgccgtcga
      241 ttttgatctc gacgatatcg cggggctgga gggtgaaggg ggtctcgggg acgatgccgg
      301 tgccggtcaa gaggaacgcg ccggtgggga agctgttgtc gcgcgcgagc cagccgatca
      361 ggtcctcgaa cgtgcgggcc atgtggtcga tgctggtgcg gccgtcgtag atgatcttcc
      421 cgccgcggtg gatgaccagg cgaatgccga tctcgctccg cggcggcatg ccgaccggca
      481 gcgtgatcca cggccccagc ccgcacgact ggttgtagca cttggcctgc ggcagataga
      541 gcgggttctc cccctcgata tcccgcgagc tcatgtcgtt cccgaccgtg aacccgacca
      601 ggtccagctt gctgctcagg accagcgtca gctccggctc cgggacgttc cacttcgaat
      661 cggcccggat ccgcagcggc tgcccgtggc cgctgacgcg gtgcggcgag gccttgaaga
      721 acagctccgg ccgcggcgag gcatagaccc ggtcgtagca cgaggccgac gtctccgatt
      781 cctccatccg ggcctgctgg ctgcgtttgt acgtcacgcc cgccgcccag acctcctgcc
      841 ggtcgatcgg cggcagaagc tgcgcctggg ccagcggaat cttctccttc gacttcgtga
      901 ggaattccgc ggcctcgttg ggattgtcgg aatccagaat ctcgaacagc gactggtatt
      961 gcccgcccga gaggtccagc ggaatgagct gatccccctc gacgcgggca acgccgtgct
     1021 ggccggtggg aagctggtat ttggcaagtt tcatgagatc gatcgtaggg tgggaccagc
     1081 gagcgaagcg agcgcaggcc caccaagatg agcgcggtgg tgggcctgcg gcctgacggc
     1141 cttgtcccac cctacgctcg ttacgccggt aatgcctcgc tcagcttccg aatccagttc
     1201 ccgtgcatga tacctgcgat atcttccggg ctgtagccgc gggtttcgaa gatgccggtg
     1261 agtttttgca ggtcggcgat cgagtcgaga tccatcggcg tctgctcggt gccgtagccg
     1321 ccgtcgaggt cggagccgat cgcgcagtgg cgcgacgtgc cagcgagctg gcagatgtgg
     1381 tcgatgtggt cggcgatcgt ggcgatcttc acgcccgttt cggtcggcgt cgtcttgccg
     1441 cgaatccagc cggggaccat catccaggcg tcgaacgccc cgccgatgac cgcgtcgcgg
     1501 cggatcagct ctttcagttg ctcgtcgctg aattgccgct ggtgcgggac gagggcccgg
     1561 cagttgttgt ggctggccca gacggggccg tcgaagattt cgagcgcttc ccagaaggcg
     1621 tcgtcggtca ggtgcgtcgc atcgagcacg aatccgatcc gccgcatttc cttcaccagc
     1681 tcgcggccgg cggccgtgag acccccttcg gcgccggtgc cggggctgta gcggccgggg
     1741 ccgtaatgag ccgggccgat cgcccgcagg ccgccggtcc aggccttttc gaggtagctg
     1801 aggttgacga tcgaatcggc cccttccagc gagaggacgt agccgatggg agcgtcggcc
     1861 ggggcgttgt tttgccagag ggcaatatgc ttctcgaggg ccgccttgtt ggcgatctgc
     1921 accatttgcc cggcgtcttc catcgcccgg tagtaggcga gctgtccctg cgtctgggcc
     1981 caggcgatct ccggcgcatg ccaccccggc agcgcatttc ccggggcgac ataacggccg
     2041 atttgcgtgg cgatgcacag gccgatctcg cccttccgca tctccgggaa ggtgcagacc
     2101 cctttgccgc gatcgacctt gtcggtcttg cccgcctcgc gctggcggac ttccgccagc
     2161 ggccgcgtca aatcgcggtt ccactcgact gcgttcatgg agagatcaag atggccgtcg
     2221 attaatagca ttttggcacc aaacggggtt tcagggacaa ccacgaatca cacgaatcta
     2281 acgaatccag gagcacagcg acggagaatc atgttagggt tccttcttct tggctacttc
     2341 gtcgtcgatt agtgcgcggc atgcctcggc acagtcacgg cagattagaa cgccttccag
     2401 tccctccgcc agcggaagct ttttctcgcc gcaataggag catcggccgc tcggcctcaa
     2461 accgaagaaa acggcgattg tcgcgataag gctcctctgc gtaccctcga tctgaggcgg
     2521 cttgtacgga ttgatgtcca ttgccagcga ccgccgccgc attcgtttga ttcgtaagat
     2581 tcgtggttta cttcgtcagt ctccagccgg ttgcatcgac cgtgaggccg gaatggtcac
     2641 ggtgttggtc gtacaccttg ttcgcgtcct cgatgctgtc gaagtagccg acgatgaagg
     2701 cggcggagaa gtgctcgccg gctttaatgg gccgctcgcc gaattcttcg atcatgcaga
     2761 cgtagccccg ctggtggcac caggcttcgc tgacgatcgc cgggtcgagc gtcatcccca
     2821 agagccacgg cccctccttg cccgtcgccg ggtcccgcag ccgatagccg cggatgatcc
     2881 gccctggcag ctttttcccg gcttcttccc aggcgtcgcg gcggtagttg aacttctcgt
     2941 cgggggcgaa gttggtgagg aactccttcg acgggatcgg ctggtcgaaa tagctcagga
     3001 agacttcgct gaacgagtcc cctgccttgt gcttgatgtg tcccggcatg tcgatccgca
     3061 ggaacatcgc ctcactgccg ttcacggcgt cgatccggtc ggaggagacg aagtaccgct
     3121 tcccctcagg aaagacgatc gtctgtgtcc aggtcgaacc ggttttcttg ccgggggcgg
     3181 cggtctggta ctggaacgac tgcgtgatcg cgacgaagtc tttcccgcga atcacctcgg
     3241 gcgagagttc cttcgccttc gtgcagatct gcggcccctc gatcgaccgc ttcggccgct
     3301 ggccgtggta gtcgttgccg aactgataga ccatttcctg cggcagcttg tcgcgatagg
     3361 ccgcatcgct ccccggctcc atgatccaat cgacgatatc cagcccgaag cccgcatcgc
     3421 gaaagccggt ctttttatcg accagcgatc ccgccgcgac ccccgagacg tagcccttcg
     3481 tgcgaatcgt cgcctccagc gtcggtgtgg cgattttgat ctcgtcgtcc gtatcgacga
     3541 cttcaatggg ttcggccgcg ggaatggagc caagggaagg aagcaggcac aacaaagcca
     3601 aaccggtgaa tcgattcatg tggaaggcat tccgtggcag gggctggatg gagggcgatg
     3661 aacttactac cgtatcgtaa gtcgcaatcg tttgttgatc caggatttgc tgggctgttt
     3721 cggctcgggc tccggctcgg acgccagcca accttccagc cacgttccga gtgacttttc
     3781 ttggtcccag aacgagtagg cccagttgcg ccgggcggct gcgccttcga gatcatcatc
     3841 caatgggttc gggtcccaga ggatcatcgg cagtttcttg tacgcgcaat cgacgcacga
     3901 ccacatcccg catccatagt tggccagcgg caacaactga cggggccagc gccaagtgcc
     3961 gttctccgtt tcgagcttga tcatgttggc gtagcacttt tcgagcgtac acccgtccag
     4021 ccttgctcca cctttgatcc ccatgatgcc gtatccgggt ccaaagccac catttcccac
     4081 cttcagataa atggcgcgga gcaattcggg gagcttgaag ccgatcgctc gctcggcagc
     4141 ctgcagcgcc tttgcattca cgggcgcaat cggtttgcga ggatggatgc cgccatctgt
     4201 cgcacgtttg cgatcggcgt tgcgctcctt gattcgtgca atcaggccct gcgcgtttgc
     4261 tgtcatcttt cggcgttggt aatgaaatcg agcagcatcg gcagctcgcc agccgggcgt
     4321 catccaacaa acagcgcgac gctcgccgtg aagccgtgca tgaagttctg cgtgccgatg
     4381 gggccgatct cgccggcggc gaagaagccg gccagcggca gcgggccgaa agcgcgggaa
     4441 atcgccgcgg catcgtggtg cggctggctg aacatccgcg tgccgcggcc gttgcaggtg
     4501 aagagcaggc cgcccagcgg cgcggcgttt tctttctgtg ctgcggaaag aagctgcttg
     4561 agctcggcgt cggcggcttc ctggtcgcgg acgtggaact gcacggtctg gccggggcgg
     4621 atgtaatcgc cgatcgccac cgcgccgctg ctggaatcga tgccgaccac gttgcggacg
     4681 agaaagtctc cctgctcgaa gtggtcccgg tattcgctca ccacgcggcc cacgtgcaag
     4741 gcccgctgca ccagccgctg ctcgctggtg ggcagctcgt caaaaatctc gcgaagctgg
     4801 atcagcgccg gccggccgcc cagctcgtaa atcacgttcc gctcggcttt ggtgatgacc
     4861 agcggccggc cgatcgggcg gcagccctgc gagacgaccg tccgcactct caccgggccg
     4921 gaaacgagga ccgcgaccgc gccttcagtc agcacctggt cgccgaacaa caggcggttc
     4981 atccccggct cgtgcccgcc gctggccatg ccgccgatga cgggcacgcc gggccggtcc
     5041 tcgttcatcc gctccagcag aaaatcagcc gggaacgtga acggatcgcc catcacgatc
     5101 agaaacgcgc ccgcgggcca ttcgccggcc agatgctcgg gccagccttc gagcgcgccc
     5161 ccttcgggcg tccgctgaaa ggccaggtgc atgggcgaga tgctcgcccc cggccagcgg
     5221 gccagccaga cggccagtgc cggcccctct tcgatctcgc acgcggtgcc gacgatcgac
     5281 tcgcccgtca cgcccagcac attcgcggtc cccagcagct cgcgcgcgtc cgcggcgatg
     5341 cgctcggcgt ccggcacgtg atgcgccgaa aagaagagca gcgccagatc gggcgcgccg
     5401 ccaagcgact cgcgggccag cgcggccgct tcgcggagcg ccgccgacgt gttttcggcc
     5461 gtcgaaattc ccgcggcgca gcgtgcggtg gacgtggtag cggctgacat catcatgctt
     5521 tcgatagaag accccaaacg cgattcgcaa ctggaatgtt agacgctggc ccacggactt
     5581 gctatcggct ggcaagctgc cggccgg
//
LOCUS       DASUGB010000859         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1345177, whole genome shotgun sequence.
ACCESSION   DASUGB010000859 DASUGB010000000
VERSION     DASUGB010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345177"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /gene="carB"
                     /locus_tag="VFV87_16330"
     CDS             <1..1553
                     /gene="carB"
                     /locus_tag="VFV87_16330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5105388.1"
                     /translation="GIEFDYCCVQAAFAMREMGIESVMINSNPETVSTDYDTSDLLFF
                     EPLTTEDVLNIHDRIEPDGVIVQFGGQTPLNLARALANAGVPIIGTSVETIEAAEDRE
                     KFAGILSRLDLKQPPNGIARTMNQARTEAAAIGFPSLVRPSFVLGGRAMEICYDMAQF
                     ERFVAEAFVVAQGQPVLIDRFLEDATEVDVDCISDGEHVVVAGIMEHIEEAGIHSGDS
                     ACAIPPYSLAGPVIQEIRDATEAMARYLRVVGLMNVQFAVKKEEGKPVVYVLEVNPRA
                     SRTVPFVAKATGVPIAKLAAKVMAGAKLPELGLVREPIPSHVSVKESVFPFRKFAGVD
                     IVLGPEMKSTGEVMGISERFSIAFAKSQLAAGTVLPKEGKIFLSVSKRHKEAVVDLAQ
                     RLVALGHEIVATEGTAARLEEAGIAATRVKKIAEGRPNLLDYMNDGQITLVLNTPSGK
                     GARTDEGRIRAAAVQLGVPCITTLQAAEAAVKAMEALREEEMTVQALQDRFPKGADAE
                     LSSQPVGV"
     gene            1724..2026
                     /locus_tag="VFV87_16335"
     CDS             1724..2026
                     /locus_tag="VFV87_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105389.1"
                     /translation="MARIAAGLLILGLAASSSGCAMCCAPFDCYYLYQGGAWVRHNPT
                     SGRVGSAFDEAGGPAIMEGQTLQGQPTPAPAEPEVLPMPTGTRSVLPQRRGQDYLP"
     gene            complement(2106..>4689)
                     /locus_tag="VFV87_16340"
     CDS             complement(2106..>4689)
                     /locus_tag="VFV87_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105390.1"
                     /translation="PLVAALQAAGPRLVTTDGFGCTSCHQVGGVLPDKAPLNARGPDI
                     SMLEKRIRREWFDRWCFSPARIVPRMEMPSVQVPVRGVLGDNVTDQLAAVWQTLNTPG
                     FEPPEPNPIRVLRLSGVPERKESPIVLHDVVKDGDKTYLFPLVIGLPNRHNILFDLET
                     GRLAAWWLGDTARQRTKGKSWWWELGGKPILSLSNGRFGIWLERRGKEILPSKAGQSA
                     ATLLESYSGRVGAASVVGIDFPLEANDSMQRLLIRDAWSVLEQRDATRESGFQRLLMC
                     DAVPPETRLCMRLVDSQTAKKCIWEESHQKLRIPNGLEIRVQPGFQGQEVKWDPDGVI
                     KVREHQKDVSIVLDYVNTLPTIDRYVIEPSPEPIRPVGSVLIAEGILGERLPLPDSLM
                     PTALSWLADGRLVFTTLKGQLVLVEENPSQQLSWRFLDDGLATPYGIQGHRTVVDVLT
                     KTGLYRVLVPLAPGEGKYVRREMLASGWGASEDYHDWAVGLVPKGNDDYFLGFPCQQD
                     QRSDAAARFRGQLLHLKSRGGLPDWPHKPRQFDIEVLSSGHRFPMGLAMNRAGDLFVT
                     DNQGNYNPFNELNHVRPGKHFGFINANEKEAGFKPPPLEEPAINIPHPWTRSVNGICF
                     LDTPVGLGRVGSAHQDSPADAKNGKAGTAHPTIYGPLEGHLIGCEYDTRRLIRMSLQK
                     IGDTYQGAAYPLSIPPDDVEKGLLGPIVCAVKPTTGELYIGEIRDSGWGAGNNVGQIV
                     KIKIEPEKLPCGIAEMKATADGFTLDFFQPVDAKKAADVSNYSLQSYRRESTPAYGGP
                     DLDRRTEKIAAIEVSKDDKRVTLKLPELRERFVYELRLKNLAPKNAMFHPDEAHFTLR
                     QVPK"
BASE COUNT          853 a         1457 c         1516 g          863 t
ORIGIN      
        1 aggggatcga gttcgactac tgctgcgtgc aggccgcctt cgcgatgcgc gagatgggca
       61 tcgagtcggt catgatcaac tcgaacccgg aaacggtctc gaccgactac gacaccagcg
      121 acctgctctt cttcgagccg ctgacgaccg aggacgtgct caacatccac gaccgcatcg
      181 agccggacgg cgtgatcgtg caattcggcg ggcagacgcc gcttaatctg gcccgggccc
      241 tggcgaatgc cggcgtgccg atcatcggga ccagcgtcga gacgatcgaa gcggcggaag
      301 accgcgaaaa attcgccggc attctctccc ggctggacct caagcagccg cccaacggca
      361 tcgcccggac gatgaatcag gcgcgaaccg aagcggcggc gatcggcttt ccgtcgctgg
      421 tgcggccgag ctttgtgctc ggcggccggg cgatggaaat ctgctacgac atggcccagt
      481 tcgaacggtt tgtcgccgag gcgttcgtcg tcgcccaagg gcagccggtg ttgatcgatc
      541 gcttcctgga agacgccacg gaggtcgatg tggattgcat cagcgacggc gagcacgttg
      601 ttgtcgcggg catcatggag catatcgagg aagcgggtat ccactcgggc gattcggcct
      661 gcgcgattcc gccctacagc ctggccgggc ccgtgatcca ggaaattcgc gacgccaccg
      721 aggcgatggc ccggtacctg cgcgtcgtgg gcctgatgaa cgtgcagttc gccgtcaaga
      781 aggaggaggg caagccggtc gtctatgtgc tggaggtcaa tccccgcgcc agccgcaccg
      841 tgccgtttgt cgccaaggcg acgggcgtgc caatcgccaa gctcgcggcc aaggtgatgg
      901 ccggggcgaa gctgccggag ctgggtctgg tgcgtgaacc gattccatcg cacgtctcgg
      961 tgaaggaaag cgttttcccg ttccgcaagt tcgcgggggt cgatatcgtc ctcggcccgg
     1021 agatgaagag cacgggcgaa gtgatgggaa tcagcgagcg gttcagcatc gcgtttgcca
     1081 agagccagct agcggccggc acggtgctgc ccaaggaagg caagatcttt ctcagcgtca
     1141 gcaagcggca caaggaggcc gtggtcgacc tggcccagcg gctggtggcg ctggggcatg
     1201 aaatcgtggc gaccgaaggg accgcggcac ggctggagga ggcgggcatt gccgcaacgc
     1261 gggtcaaaaa gatcgccgaa ggccggccga acctgctgga ctatatgaac gacggtcaga
     1321 tcacgctcgt cctgaatacc ccgagcggca agggcgcccg caccgacgaa ggccgcatcc
     1381 gcgccgcggc ggtgcagctc ggcgtcccgt gcatcacgac gttgcaggcg gccgaagccg
     1441 ccgtcaaagc gatggaggcc ttgcgcgagg aagaaatgac cgtgcaagcc cttcaggacc
     1501 gctttcctaa gggggctgac gcggagcttt cgagccaacc ggtgggcgtg tagatcgctt
     1561 cagtcgcccg caaagtcgcc ataatccgca aagtttgcct aaagcttgca ggtggcagcg
     1621 accgaaaatc ggagggacgc cgggatgccg cgcgccaaca tctggttccc aagctccagc
     1681 ttgggaacca gcgcgacgct gaaccgtttg caaggatgct gtcatggctc gaattgctgc
     1741 cggattgctg attctgggcc ttgccgcgtc aagcagcggc tgcgcgatgt gctgtgcccc
     1801 gttcgactgc tactaccttt atcagggtgg cgcctgggtg cggcacaatc cgacgagcgg
     1861 acgcgtgggg agcgccttcg acgaagccgg cgggccggcc ataatggaag ggcagaccct
     1921 gcaaggtcag ccgacacctg ctccggccga acccgaggtg ctgcccatgc ccaccggcac
     1981 gcggagcgtg ctgccgcagc ggcgagggca agattatctg ccgtaggttc cgaccgcctg
     2041 ggcggaacct gagttgccgc tcaatcctcc acacgcccgc tgggtcctgc cgaggccgtc
     2101 aggacctact tcggcacctg ccgcagggtg aagtgggctt cgtcgggatg gaacatcgcg
     2161 tttttcgggg cgaggttctt cagtcggagc tcatagacga accgctcgcg cagctcgggc
     2221 agcttgagcg tcactcgctt gtcgtccttt gagacttcga tggcggcgat cttctccgtc
     2281 cgccggtcga gatcgggccc gccgtaggcc ggcgtcgatt cgcggcggta cgattgcagc
     2341 gaatagttgg agacgtcggc cgccttcttc gcgtccaccg gctggaagaa gtcgagcgtg
     2401 aagccatcgg ccgtcgcctt catctcggcg attccgcagg ggagcttctc cggctcgatc
     2461 ttgatcttca cgatctggcc gacgttgttc cccgcccccc aaccgctgtc gcggatttcg
     2521 ccgatataca gctcgccggt cgtgggctta accgcacaca cgattggccc gagcaggccc
     2581 ttttcgacat cgtcgggcgg aatgctcagc ggatacgccg ccccctggta cgtatcgccg
     2641 attttctgca gcgacatccg aatcagccgg cgggtatcgt actcgcagcc gatgaggtgg
     2701 ccttcgaggg ggccgtaaat cgtagggtgg gctgtgcccg ccttcccatt cttcgcatcg
     2761 gccggcgaat cttggtgggc agagcccacc ctaccgaggc ctacgggcgt gtccaggaag
     2821 caaatcccgt tgacgcttcg cgtccaggga tgcggaatgt tgatcgccgg ctcttcgagc
     2881 ggcggcggct tgaagcccgc ctccttctcg ttcgcgttaa taaacccaaa atgcttccct
     2941 ggccggacgt ggttcagctc attgaacggg ttgtagttcc cctgattgtc ggtcacaaac
     3001 aagtcccccg cccgattcat cgccaagccc atcggaaagc ggtggccgga ggagaggact
     3061 tcaatgtcga attggcgcgg cttgtgcggc caatctggca gacctccgcg agacttcaag
     3121 tgcagcaatt gaccgcgaaa gcgcgcggca gcgtcgctgc gctggtcctg ctggcacggg
     3181 aaccccagaa agtagtcgtc gttacccttt ggcaccaggc cgacggccca atcgtggtag
     3241 tcctcgctgg cgccccagcc agaggcgagc atttcacgtc ttacatactt gccttctccc
     3301 ggcgcaagcg ggacgaggac acggtacagc ccggttttgg tcagcacatc aacgacggtc
     3361 cgatgtcctt ggatgccata aggggttgca agcccatcgt cgagaaaacg ccaactgagc
     3421 tgctggcttg gattctcttc caccaggaca agttgaccct tcaatgtcgt gaacaccagc
     3481 cgcccgtcag ccagccaact aagtgcggtg ggcatcagac tgtcgggcag tggcaatcgc
     3541 tcgcccagaa tgccttccgc gatcagcaca ctcccgaccg gtcgaatagg ctcgggggac
     3601 ggctcaatca cgtaccgatc gatggtgggc agcgtgttca catagtccag aacaattgac
     3661 acgtcttttt gatgctcgcg gaccttgatc acaccatccg ggtcccattt tacctcctgg
     3721 ccctgaaacc ccggctgcac gcgaatttct aatccattgg gaatccgcag tttctgatgc
     3781 gattcttccc aaatgcactt cttggctgtc tgcgaatcga cgaggcgcat gcagaggcgc
     3841 gtttctggag gaaccgcgtc gcacattagc agtcgttgga atcctgattc tcgggtcgcg
     3901 tcgcgttgtt caaggacgga ccacgcgtcg cggatcaaga gacgctgcat tgagtcgttc
     3961 gcttcgagtg ggaagtcaat accaaccaca gatgccgcac caacccggcc ggagtacgac
     4021 tccaggagag tcgccgcgga ttgtcccgct ttggagggca aaatctcttt gccacgcctt
     4081 tcgagccaga ttccgaaccg cccgtttgaa aggctgagga ttggtttccc tcccaattcc
     4141 caccaccaac tcttcccttt cgtccgctgg cgggcggtgt cgccgagcca ccaggcggcg
     4201 aggcggcctg tttcgagatc gaacaggatg ttgtgccggt tgggcaggcc gatgacgagc
     4261 gggaagaggt aggttttgtc gccgtccttg accacgtcgt gcaggacaat cggcgattcc
     4321 ttccgctcgg gaacgccgga gaggcgcagg acgcggatcg ggttcggctc cggcggctcg
     4381 aagcccggcg tgttgagcgt ctgccagacg gcggcgagct gatcggtgac gttgtcgccg
     4441 aggacgccgc gcaccgggac ctggacgctg ggcatttcca tccgcggcac gatccgcgcg
     4501 gggctgaagc accagcggtc gaaccactcg cggcggatgc gcttttccag catcgaaatg
     4561 tccggcccgc gggcgttgag cggcgccttg tccggcagca cgccgcccac ctggtggcag
     4621 ctcgtgcagc cgaagccgtc ggtggtgacg agtcgcgggc cggccgcttg caaagccgcg
     4681 accagtggt
//
LOCUS       DASUGB010000860         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1345330, whole genome shotgun sequence.
ACCESSION   DASUGB010000860 DASUGB010000000
VERSION     DASUGB010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345330"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFV87_16345"
     CDS             <1..1041
                     /locus_tag="VFV87_16345"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5105391.1"
                     /translation="VKLSVPEMEVDVAQKQALIDQAAAEVAQAEAAKKAAESAVTTAQ
                     SKVTQAEALVARTQADIERWQSEFARYEQLVTGGSVNRQLLDETKQKLRAAEASRTEA
                     DAGIAAAKAALAQAESGVVQAESDIVAAKARQRVAEANRTYAQTLLAYAEIKAPFAGI
                     VTQRQVDPGHYVQPAGPEAAPLLVVARTNVARIFVAVPELEAGYVDLGDEVTVSVQSL
                     RGEQFPGKVTRTSVVLDDSSRSLLTIIDIDNADGRLRPGTFATAKIKLDEHQDVLTLP
                     AAAVVRQDREAFCYRLIDGKAAKTRIQLGLNVGGEWEITSGLSDGDTVILNKANALQD
                     AQPVEVLKPAAG"
     gene            1157..2128
                     /locus_tag="VFV87_16350"
     CDS             1157..2128
                     /locus_tag="VFV87_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5105392.1"
                     /translation="MLARFPNLMLPVGIVVALTAPAALAAQDIHPVAFLRLGDCPEVV
                     CPCEEEEEECLRSFGPCCCEVRKTLMQWSYGTSFSGGPDLEEPIVTDRPDFTEASVTV
                     GRGVLQLEMGYTFFSDTTGGVTTRSQSFPEALFRYGIFADWFELRLAQNIGGEDDGFL
                     LQNTGAEDLYLGVKLALTPQEGILPEMAIIPQMTVPTGHPVFTAGEVLPGVNWLYGWD
                     INDFLAMGGSTQFNRTIDEITGEAYTEWAQSWTINYSLTDRLGAYTEWFAFFPHSAST
                     ATPQHYFDGGFTYLLTDNIQWDIRGGVGLNEAADDFFVGSGLSIRWK"
     gene            complement(2145..2339)
                     /locus_tag="VFV87_16355"
     CDS             complement(2145..2339)
                     /locus_tag="VFV87_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105393.1"
                     /translation="MVWVNVDLGSSELWEESAGDPGTSWQLTECEGFGLPLVGTILGL
                     GQARDEPLDRIDAPGNRRWN"
     gene            complement(2373..2714)
                     /locus_tag="VFV87_16360"
     CDS             complement(2373..2714)
                     /locus_tag="VFV87_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105394.1"
                     /translation="MRSTAIVLIVLLAAGSLAVTASAGGIIQGHNCPHCGHHQPCPAV
                     PVTIKEKKHCWQVECKNFCIPPIKWPWDHCCEPGCGKVKTVKVLKKIEYECARCGYEW
                     EAPSVCCECGK"
     gene            complement(2739..>3051)
                     /locus_tag="VFV87_16365"
     CDS             complement(2739..>3051)
                     /locus_tag="VFV87_16365"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5105395.1"
                     /translation="ELGILDRVQPFENFFLVDTCDDGVAGGWGAWQIALRYSHANLSN
                     QDIRGGIGNELTFDMNWYWNAYSKMQFNAIYGEITDRQPVDGQTDAFFTILGTRFIVD
                     F"
BASE COUNT          611 a         1031 c          852 g          557 t
ORIGIN      
        1 gtcaagctct cagtacccga gatggaagtc gatgtggcgc agaagcaggc gctcatcgat
       61 caggccgccg ctgaagtcgc gcaggcggaa gcagcgaaga aggccgcaga atccgccgtc
      121 acaacggccc agtcgaaggt cacgcaagcc gaggccctgg tcgcccgtac gcaggccgac
      181 atcgagcggt ggcagtcgga attcgcccgc tacgaacagc tcgtcaccgg cggctcggtc
      241 aaccggcagc ttctggacga aacgaagcaa aagctccgcg ccgccgaagc gagccgcacc
      301 gaagccgacg ccgggatcgc tgccgcgaag gccgcgctgg cccaggctga gtccggcgtc
      361 gtacaggccg aatcggacat cgtcgcggcc aaggcccggc agcgcgtcgc cgaggccaat
      421 cgcacctacg ctcagacgct cctcgcctat gctgaaatca aggcgccctt tgcggggatt
      481 gtcacgcagc gtcaggtcga tccgggacat tacgtccagc cggctggtcc ggaggctgcg
      541 ccgctcttgg tcgtcgcacg aaccaatgtg gcacgcatct tcgtcgccgt gcccgagctc
      601 gaagcgggct acgtcgatct cggcgatgaa gtcacagtct ccgtccaatc gctccgcggc
      661 gagcagttcc ccggcaaagt cacacgcacc agcgtcgtcc tcgacgactc cagccgctcg
      721 cttctgacaa tcatcgatat cgacaatgcg gatgggcgcc tgcggccagg cacgttcgcc
      781 acggccaaga tcaagctcga tgagcatcaa gacgtgctga ccctccccgc tgccgccgtc
      841 gtacggcagg atcgcgaagc cttctgctac cggctcatcg atggcaaggc ggcgaaaacg
      901 cggattcagc tcggcctgaa tgtcggcggc gagtgggaaa tcaccagcgg cctctcggat
      961 ggcgacacgg tcattctcaa taaggccaat gccctgcagg atgcccagcc agtggaagtt
     1021 ctaaagccgg ctgctggctg atagcgatcc gcttgcattt tgagtcatca aatttgactc
     1081 tccaaaacct tacgctttag aaagctgcgg ctaggcgtcg aatggattag ttaggcgaaa
     1141 cgtcgccaac ttgcctatgc tcgcgcgctt tccaaatttg atgcttcccg tggggatcgt
     1201 cgtcgcgctc acggcacccg ccgcgctcgc cgctcaggac atccaccccg tggccttcct
     1261 gcgcctgggc gattgccccg aggtggtctg cccttgcgag gaagaagagg aggagtgcct
     1321 ccgctctttc ggcccgtgct gctgcgaggt ccgcaagacg ctcatgcagt ggagctacgg
     1381 caccagcttc tccggcgggc ccgatctcga ggaaccgatc gtcacggacc gcccggactt
     1441 caccgaagcc agcgtcaccg tcggccgcgg tgtgctgcag ctcgaaatgg gctacacatt
     1501 cttttccgac accactggcg gcgtaaccac tcgcagccaa tcctttcccg aggccctttt
     1561 ccgctatggc atcttcgccg attggttcga gctgcgactg gcgcaaaaca tcggtggcga
     1621 agacgacggc ttcctcctgc aaaacacagg cgccgaagac ctctacctgg gcgtcaagct
     1681 ggctctcacg ccgcaggaag gcatcctgcc cgagatggcg attatcccgc aaatgaccgt
     1741 gcccaccggc catcccgtct tcaccgctgg cgaagtgctc cccggcgtca actggctgta
     1801 cggctgggat atcaacgact tcctcgcgat gggcggcagc acacagttta accgcacgat
     1861 cgatgagatc accggcgagg cctacaccga atgggcccag tcatggacga tcaactattc
     1921 gctcaccgat cgcctcggcg cctataccga gtggttcgcc ttcttcccgc acagcgccag
     1981 taccgccacg ccgcagcact atttcgacgg cggcttcacc tatctcctca ccgacaatat
     2041 ccagtgggat attcgcggcg gcgtcggatt aaacgaggcg gctgacgact tcttcgtcgg
     2101 cagcgggctt tcgattcgct ggaaatagtt cgcgccgcag cacgtcagtt ccagcgccgg
     2161 ttgcccggcg cgtcgattcg atccaacggc tcgtcacgag cctggcccag ccccagaatc
     2221 gtccccacca gaggcaaccc aaagccctcg cactcggtca attgccagct cgtgccagga
     2281 tcgccagcag actcttccca tagttccgag ctacccagat ccacattcac ccacaccatg
     2341 cacgattcct cgcaaacgag taatgacgca acttacttac cgcattcgca acagacgctc
     2401 ggcgcttccc attcatagcc gcaccgcgcg cactcatact cgatcttctt cagcaccttc
     2461 acggtcttca ctttgccgca gcctggttcg cagcaatggt cccacggcca cttgatcggc
     2521 gggatgcaga aattcttaca ctccacctgc cagcagtgct tcttctcctt gatggtcacc
     2581 ggcacggcgg gacacggctg atggtggccg cagtgcggac agttgtggcc ctgaatgata
     2641 ccgccggccg aggccgtgac cgcgagcgac cccgccgcca gtaacactat gagaacgatc
     2701 gcagtgctcc gcatcgcaag acctccttgg cggcgagcct aaaaatcgac aatgaatcgt
     2761 gtccccaata tcgtgaagaa cgcgtccgtc tggccatcga ccggctggcg gtcggtaatt
     2821 tcgccataga tcgcgttgaa ctgcatcttc gagtacgcat tccaatacca gttcatgtcg
     2881 aaggtcagct cgttgccgat cccgccacga atgtcttggt ttgacagatt ggcgtgcgaa
     2941 taacgcagcg cgatctgcca cgcaccccac ccgccagcca ctccgtcgtc gcacgtatcg
     3001 accaggaaga aattctcgaa cggctgcaca cgatcgagaa ttccgagctc a
//
LOCUS       DASUGB010000861         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_790135, whole genome shotgun sequence.
ACCESSION   DASUGB010000861 DASUGB010000000
VERSION     DASUGB010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790135"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2031
                     /locus_tag="VFV87_16370"
     CDS             <1..>2031
                     /locus_tag="VFV87_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX5105396.1"
                     /translation="INPAAEFPACGAIDVNKDGKLDIVSGGFWYEAPKWTKHFLREVE
                     VIRGRFDDYSNLEYDVNADGWTDIISVNYRSKSLFWMEHPGEAIRANSETPWAKHIID
                     APGPMETGRLHDIDGDGRLDILPNGTDFAAWYEVVPGRQPQFLKHDLPQEIAGHGVGF
                     GDINGDGRGDIVGPKGWLEAPPDRRAGRWLWHPDWELHRDASIPILVHDVNGDGDNDL
                     VYGRGHNYGLYWVEQASHAGATAGSAGATAGSSGATAGSPSSGPRNWTRHAIDTSWAQ
                     AHSLLLVDLDGDKRLEVVAGKRYMGHEGKDPGEYDRIEHYAYTFDPHSRSFQRRLIAQ
                     DCAGFGLDPKAVDIDGDGDLDLVAADRNGLSLLVQGPPAGAAKSAPAPAERPDRFQLA
                     NWLGRPIANPADWRPRKEAIVAGMVQVMGALPDLARRVPLDVKIESREETPDYVRIKL
                     TYVPEPGDRVPALLLVPKSVPLAKDGIKRDVIATTGRGNVSGATTGRGNARAPAMLCL
                     HPTNPDGKAQTAGLVGEATRHYGHQLAQQGFVCLIPDYPSFGDYKSYDFHQQRPGSDE
                     PLYVSGTMKAIWNNVRAVDLLESLDYVDPERIGAIGHSLGGHNALYTAVFEPRIKAVV
                     TSCGFNAFHDYYKGNLKGWTSDRYMPRIRDVYHNDPDQMPFDFPEVLAAIAPR"
BASE COUNT          379 a          667 c          659 g          326 t
ORIGIN      
        1 atcaacccgg cggcggaatt cccggcctgc ggcgcgatcg acgtgaacaa ggacggcaag
       61 ctcgacatcg tcagcggcgg gttctggtac gaggccccaa agtggacgaa gcacttcctc
      121 cgcgaggtcg aagtcatccg cggccgcttc gacgactatt ccaacctgga atacgacgtg
      181 aatgccgacg gctggaccga catcatcagc gtcaactacc gcagcaagag cctgttttgg
      241 atggagcacc ccggcgaggc gatcagggcc aactcggaga cgccctgggc caagcacatc
      301 atcgacgcgc ccgggccgat ggaaaccggc cgcctgcacg acatcgacgg cgacgggcgg
      361 ctcgacatcc tgcccaacgg caccgatttc gccgcctggt acgaagtggt ccccggccgc
      421 cagccgcagt tcctcaagca cgatttgccc caggagattg ccgggcacgg cgtcggcttt
      481 ggcgacatca acggcgacgg ccgcggcgat atcgtcggcc ccaagggctg gctggaagcc
      541 ccgccggatc gccgcgccgg ccgctggctc tggcatcccg attgggaact gcaccgggac
      601 gcgagcattc cgattctcgt gcacgacgtg aacggcgacg gcgacaacga cctggtctat
      661 ggccgcggac acaattacgg gctgtactgg gtcgagcaag ccagccacgc gggtgccact
      721 gctggctctg cgggtgccac tgctggctct tcgggtgcca ccgctggctc gcccagcagt
      781 ggaccgcgga attggacccg ccacgccatc gacacgagct gggcccaggc ccacagcctc
      841 ttgctcgtcg atctcgacgg cgataaacgg ctggaggtcg tggccggcaa gcggtacatg
      901 gggcacgagg ggaaggaccc cggcgaatac gaccgcattg aacattacgc ctacacgttc
      961 gacccgcatt cgcggtcgtt tcagcggcgg ctgatcgcgc aagactgcgc cggcttcgga
     1021 ttggacccca aggcggtgga catcgacggc gatggcgacc tggacctggt ggccgccgat
     1081 cggaacgggc tctcgctgtt ggtgcaaggc cctccggccg gcgccgcgaa gtccgcgcca
     1141 gcgccggctg aacgcccaga ccgttttcaa cttgccaatt ggctcggcag gccgatcgca
     1201 aatcccgcgg attggcggcc gcgaaaggaa gcgatcgtcg cgggcatggt tcaagtgatg
     1261 ggggcgcttc ccgacctcgc gcggcgcgtg ccgctggacg tcaagatcga gtcgcgcgag
     1321 gaaacgcccg attatgtgcg gatcaagctg acgtacgtgc ccgagccggg cgaccgcgtg
     1381 ccagcgctgc tcctggtgcc gaaatccgtg ccgctcgcga aggacggcat caagcgggat
     1441 gtcatcgcga ccactggtcg cggcaatgta tctggcgcaa ccactggtcg cggcaatgca
     1501 agggcgcccg ccatgctctg cctgcacccg accaatcccg acggcaaggc gcagactgcc
     1561 ggcctggtag gagaggcgac gcggcactat ggccaccagc ttgcccagca aggcttcgtc
     1621 tgcctgattc ccgattatcc gagttttggc gactacaaga gctacgactt tcatcaacaa
     1681 cggcccggca gcgacgagcc gctctatgtg agcggcacga tgaaggcgat ttggaacaat
     1741 gtgcgggccg tcgatctgct cgaatcgctc gactacgtcg atcccgagcg aatcggcgcc
     1801 atcggccatt cgctcggcgg gcacaacgcc ctctatacgg ccgtctttga gccgcggatc
     1861 aaggcggtcg tcaccagctg cggcttcaat gcctttcacg attattacaa ggggaatctg
     1921 aagggctgga cgagcgatcg ctacatgccg cggattcgcg acgtgtatca caacgacccg
     1981 gaccagatgc cgtttgattt tccggaggtg ctggcggcca tcgccccgcg g
//
LOCUS       DASUGB010000862         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_929964, whole genome shotgun sequence.
ACCESSION   DASUGB010000862 DASUGB010000000
VERSION     DASUGB010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929964"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1043)
                     /gene="eda"
                     /locus_tag="VFV87_16375"
     CDS             complement(396..1043)
                     /gene="eda"
                     /locus_tag="VFV87_16375"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX5105397.1"
                     /translation="MPASTPLDRILDRVIVAVIRAESPDSLVDVAEALLAGGVDVMEV
                     TFTVPRAARVLESVASKLGDRILLGAGTVLDPETARVALLAGAEFIVSPAVNPQVIEL
                     CRRYSKVVMPGALTPTEVVAAWQTGADIIKIFPSELTGPRYIKLLHAPLPQVRLMPTG
                     GVNAQTAGEYLAAGACALGVGGALVDPRLVAAGDLDEISRRASQLVEAVKKARNP"
     gene            1239..>2316
                     /gene="purH"
                     /locus_tag="VFV87_16380"
     CDS             1239..>2316
                     /gene="purH"
                     /locus_tag="VFV87_16380"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5105398.1"
                     /translation="MDRVPLLCALISVSDKTGLADLACGLSAGGVEILSTGGTRKFLV
                     EHGFEVRDVAAYTGISEMMDGRVKTLHPKIFGGILARRDVPEDLHSAKEHGIELIDLV
                     VVNLYPFERTIAKPEVAPAEAIENIDIGGPSLVRAAAKNHAFVAIATQPEQYGEILQE
                     LKASGGTSLALRQKLAGAAYAHTAAYDAAIAGWFGAQAGEMFPPTMQVSLKKRMELRY
                     GENSHQRGAVYAAPGCSGANLIAARQLNGKELSYNNLLDLDAALTIVRLLDRPAAAVI
                     KHNNPCGAATASTLAEAAKKALAGDPQSAFGGVLGFNRPFDAAAAELLAAPGLFIEAI
                     VAPDFEPAALEILTTRPKWKANVRL"
BASE COUNT          464 a          709 c          732 g          411 t
ORIGIN      
        1 agatactccg tcgtccaggt cagcagccgg aggacagtcc atggatcggg ctgcgacata
       61 aaagaaatgg agtgcaggag gataccaaaa cgcggctcgg ctgaattgta caggttcgac
      121 cgtggggtat cgatcgcggt ggcgtacctc gacggtgccg ggtaaaatgg aggaggagga
      181 tattcgtgca accgatcgtc gcaacgtatg ccaacggtgt cttcacgcct gtcgagccca
      241 tccaactgcc ggagggattt cgtgtaacac tctggctcga cccacttacg gtgcagcctc
      301 ctgcagaacc cagtaacacc gatcggcaat acctcgaaga gatggccgaa aatcgaggcg
      361 aggttctgcg tcgaatgagt gagtaatcgt cttgatcacg gattgcgcgc ttttttgacg
      421 gcttcgacaa gctgcgaggc gcgacgcgaa atttcatcca gatcgcccgc ggccaccagc
      481 ctcggatcca cgagcgcgcc gccgacaccc aaagcgcagg ccccagccgc aagatactct
      541 ccggccgtct gagcgttgac gccgccggtc ggcatcagcc gcacttgcgg gagaggggcg
      601 tgcaaaagtt tgatatagcg aggcccagtc aattcgctgg gaaagatctt gatgatatcc
      661 gcgccggttt gccaggcagc gacgacttcc gttggcgtga gggccccggg catcacgacc
      721 ttgctgtatc gccggcacag ctcgatcact tgcggattca ccgcgggaga aacgatgaat
      781 tccgcgccgg ccagcagggc cactcgtgcg gtctcgggat cgagcaccgt tccggccccc
      841 agcagaattc gatcgcccaa cttgctggca acgctctcca gcacgcgtgc cgcccgtgga
      901 acggtaaacg tgacttccat cacgtccacg ccgccggcca ggagcgcttc agccacatcg
      961 acgagcgaat cggggctctc cgcgcgaatc acagcgacaa ttacgcggtc aaggattcgg
     1021 tcgagtggag tggatgctgg cataatcgac tcgtatcaca ttggtcggaa tcgacatcgt
     1081 ccggcagtat tgtactcgtt gccgctggcg tcgaaatgcg ttgtggcaaa aggcactaca
     1141 tgcaacgcaa ttcagcaaac agcttacgga ccttgaccgc ccgtcgtccg ggctgagaat
     1201 ggcacgttct ggcacttact cgtatgcaga cgattgcgat ggatcgagtt cctttactct
     1261 gcgctttgat cagcgtcagc gacaagactg gtttagccga ccttgcttgc ggactctccg
     1321 cggggggcgt ggaaatcctc agtaccggcg gcacgcgaaa attcctcgtg gagcatgggt
     1381 tcgaagtccg cgatgtggcc gcctataccg gcatttcgga aatgatggac ggccgcgtga
     1441 aaacgctgca ccccaagatt tttggcggaa tcctggcccg ccgcgacgtg ccggaggacc
     1501 tgcactcggc gaaggagcac gggatcgagc tgatcgacct cgtggtcgtc aatctgtatc
     1561 cctttgagcg gacgatcgcc aagccggaag tggctcccgc cgaggccatc gagaatatcg
     1621 acatcggcgg ccccagcctg gtgcgggccg cggcgaagaa tcacgcgttc gtcgccatcg
     1681 ctacgcagcc ggagcagtac ggcgagattc tgcaggaact aaaggcgagc ggcggcactt
     1741 cgctcgccct gcgccagaaa ttggccggcg cggcgtacgc gcacacggcg gcctacgacg
     1801 ccgcgatcgc cggctggttc ggcgcgcagg ccggcgagat gtttccgccg acgatgcagg
     1861 tgtcgctgaa gaagcggatg gagctgcgct acggcgagaa ttcgcatcag cgcggcgcag
     1921 tgtatgccgc gccgggctgc agcggcgcga acttgattgc cgcccggcag ctcaacggca
     1981 aggagctgtc gtacaacaac ctgctcgatc tggacgcggc tttgacgatc gtccgcctgc
     2041 tcgatcggcc ggccgcagcg gtcatcaaac acaacaatcc gtgcggcgca gcgaccgcct
     2101 ccacgctggc ggaagccgcg aagaaggccc tcgccggcga tccgcaaagc gccttcggcg
     2161 gcgtcctcgg cttcaatcgg ccgttcgatg cagcggctgc cgaattgctc gccgcgcccg
     2221 gcctgtttat cgaggcgatc gtcgcgccgg atttcgagcc ggcggcgctg gaaatcctga
     2281 cgacgcggcc caagtggaag gcgaacgtgc ggttga
//
LOCUS       DASUGB010000863         6176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1345753, whole genome shotgun sequence.
ACCESSION   DASUGB010000863 DASUGB010000000
VERSION     DASUGB010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345753"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFV87_16385"
     CDS             <1..674
                     /locus_tag="VFV87_16385"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5105399.1"
                     /translation="VDPPDDFRDSNPPSNAALLDALAKDFADHNYDRKHVLRSILNSR
                     TYQADFRTNDFNKDDVKYFSHYQPRLLSAEQLLDAICAMTALPETFAGLPAGMHATEL
                     PAPDIAKHEFLKIFGQPERQTVCACERTSDSNLGMAIQFFNGPLIYGKLRDANNRFRK
                     ALAAGKSNEEIITELYLAAVCRKPSPKELEASLAHIAAKGDQIVALEDIGWAILNTNE
                     FLFQH"
     gene            748..2091
                     /locus_tag="VFV87_16390"
     CDS             748..2091
                     /locus_tag="VFV87_16390"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5105400.1"
                     /translation="MHSRLSLLALFLALAAPAATQADPISFKRDIAPILLDNCLACHG
                     PKKAEGGYRIDTYERVISEGDSTVGGFIAKDVETSEAFRRITSPDVKERMPLEGDPLP
                     AEQVDLLKRWIEEGAAFDGPDPKAALASYIPPPTHPAAPDPYKGTMPVTAVEFSPDGS
                     QLLVGGYHEIVAWNPADGSLIRRIGNVGQRTYAIRYSHDGQLLAVACGAPGRLGEVRI
                     FKPDTGELVRVLGMTSDVVFDCAFSPQGDRLATAAADGAVRVFNVADGAEQLTITSHS
                     DWAFAVAWKADGSQLASASRDKTAKVFDAKTGDLLITYSGHNQPVRGVLFHPDGAEVY
                     SSGSDNKVHRWKIADAAKSAEIGFGGEVYKLQGAGEFFVTASAENKVRQFKAKDQAQV
                     REFAGPKDWVLSAAMHEGTKRVAGGTFDGQVFVWSLDDGAVVKSWFAAPGYTPPQ"
     gene            2212..3039
                     /locus_tag="VFV87_16395"
     CDS             2212..3039
                     /locus_tag="VFV87_16395"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX5105401.1"
                     /translation="MQGLYEKVEAAAQTIRSVWKTSPHAGVILGTGLGGLTQHIKEEA
                     SLEYEEIPGFPKSTATSHRGRLVCGKLGELPVVAMEGRLHMYEGYDLNQVTLPVRVMK
                     AIGAKLLVVSNACGGMNPLYALGDIMVIDDHINLMGANPLIGINDDRLGPRFPDMSQP
                     YDCKLIDRALEIARRENIVAHRGVFVAVSGPNLETRAEYRFLRMIGADVVGMSTVPEV
                     IVAAHAGLKVVGFSIVTDMCLPDALEPADVPKIIATANAAEPKLTKLVLGVLAGESV"
     gene            3107..3979
                     /locus_tag="VFV87_16400"
     CDS             3107..3979
                     /locus_tag="VFV87_16400"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX5105402.1"
                     /translation="MDLGPALAAIRRHWKGQAATGVILGTGLGEIAEEVAADAIIPYR
                     EIPGFPYSTAMAHKGRLVCGLLNGTPVVMLQGRCHLYEGYSLAAVTFPTRVLHALGIQ
                     TLIVTNAAGGINPQYAVGDVMLIDDHINLMWLPSVGQASSLPKIRHIAAANLYDDELA
                     HLSLAIARRANFLLHRGVYVAVTGPSYETRAEYRAFRRIGGDCVGMSTVPEVLTAASL
                     GLRVLGLSTITNVARPDAPQTVSAEEVVHVAASALPKVRAIIHGMLNHMHQTGANSLR
                     NPAASVPPAVANSD"
     gene            complement(3980..5443)
                     /locus_tag="VFV87_16405"
     CDS             complement(3980..5443)
                     /locus_tag="VFV87_16405"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX5105403.1"
                     /translation="MRRSPLFLAALATIVIVGSARADVRLPKIFGDHMVLQQQTEAPV
                     WGWADAGEEVTVTLGEAKASTKAGADGKWMVKIKTPAASETALELVVKGTNELKLTDV
                     LAGEVWIASGQSNMEWSVAQSTNAAEEIKNANYPQIRMIKVGRNPSDKPLEDFEFVPN
                     PKGATSGWAVCSPETAAPMSAVGLFFARKLNQELKVPIGMISTNWGGTLAEAWTSHET
                     LASDPEWFGPILERDKQFKPGNPNQAGVLYNSMIQPLIPFAIKGAIWYQGESNVSRAE
                     QYAKLFPAMIADWRKHFGQGDFPFLFVQLAPYIYDRAGMKDTGQLPELWEAQLKTLTA
                     SPNTGMAVTTDIATIRNIHPPNKQDVGARLALWALAGSYGKSDLDYSGPLYDSMAIEG
                     NKIRIKFKHAAGLKFDPSKPGEHFTIAGEDMKFVPAKAEIDGETIVVSSSDVAKPVAV
                     RFGWTDTSEPNLFNGAGLPASPFRTDSFPMVTADRRR"
     gene            complement(5540..6070)
                     /locus_tag="VFV87_16410"
     CDS             complement(5540..6070)
                     /locus_tag="VFV87_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5105404.1"
                     /translation="MAAKQPLIGKRILTFVGDSYEDLELWYPKLRLIEAGAAVTVAGE
                     KSGEKYEGKNGYPCVADAAIVEMRAADFDGLVVPGGFMPDKLRRDPDVLQLVRDFDVA
                     GKLIAAICHGGWIPISAGVYRGVRVTGSLGIKDDLVNAGAVWEDAAVVVDRHFVSSRK
                     PDDLPDFCRGILQVLG"
BASE COUNT         1121 a         1987 c         1923 g         1145 t
ORIGIN      
        1 tcgtcgatcc gccggacgat ttccgcgatt ccaacccgcc cagcaacgcc gctttgctcg
       61 atgcgctggc caaggacttt gctgaccaca actacgaccg gaagcacgtc ctgcgatcga
      121 tcctgaatag ccgcacgtac caggccgatt tccgtaccaa cgacttcaac aaggacgacg
      181 taaagtactt ctcgcactat cagccgcggc tgttgtcggc cgaacagctt ttggacgcga
      241 tttgcgcgat gaccgcgctg cccgagacct ttgccggcct gccggcggga atgcacgcca
      301 cggaattgcc ggcacctgac atcgccaagc acgaattcct caaaatcttc ggccagccgg
      361 agcgacagac ggtttgcgca tgcgagcgga cgagcgattc gaacctcggc atggcgatcc
      421 agttcttcaa cggcccgctg atttacggca agctgcgcga cgcgaacaac cgcttccgca
      481 aggccctggc agccgggaag tcgaacgaag agatcatcac cgagctctac ctcgccgccg
      541 tttgtcgcaa gccctcaccg aaggagttgg aagccagcct ggcccacatc gcggccaagg
      601 gcgaccagat cgttgccctg gaggacatcg gctgggcgat tttgaatacg aacgagttcc
      661 tgtttcaaca ttgatatctg aagggaacgc cctctgtggc gttcctcggg gcaggcgtcc
      721 agcctgccaa gattggagtc ccaccgtatg cactcccgcc tctcgttact cgctcttttt
      781 ctggccctcg ccgcgcctgc ggcgacgcag gccgacccca tcagcttcaa gagggacatc
      841 gccccgatcc tgctcgacaa ctgtcttgcc tgccacggcc cgaagaaagc cgaaggtggc
      901 taccggatcg atacctatga gcgcgtgatc tccgagggtg attcgacggt tggcggcttt
      961 atcgccaagg acgtggaaac gagcgaggcc ttccgccgga tcacttcacc ggacgtgaaa
     1021 gagcgcatgc ccttggaagg cgatccgctg ccggccgagc aggtcgatct gctgaagcgg
     1081 tggatcgagg aaggcgcggc gttcgacgga ccagatccca aagcggccct cgcctcgtac
     1141 attccgccgc cgacgcatcc ggctgcaccc gatccgtaca agggcacgat gccggtcacg
     1201 gccgtcgagt tcagccccga tggctcccaa ctccttgtcg gcggctatca cgagatcgta
     1261 gcctggaatc cggcggatgg ctcgctgatc cgccgcattg gcaacgttgg ccagcggacc
     1321 tatgcgattc gctacagcca cgacgggcaa cttttggccg tggcctgcgg cgcgccgggt
     1381 cgtttgggcg aggtgcggat tttcaagccg gataccggcg agctggtgcg ggtgctggga
     1441 atgaccagcg acgttgtctt cgactgtgcc ttcagcccgc agggggatcg gctggcgacg
     1501 gcggctgccg acggggccgt gcgcgtgttc aacgtggctg acggcgccga gcagctcacc
     1561 atcaccagcc acagcgattg ggccttcgcc gtcgcctgga aagccgacgg ctcgcagctc
     1621 gcatcggcca gccgcgacaa gacggccaag gtgttcgacg cgaaaaccgg cgacctcttg
     1681 atcacctatt ctggccacaa ccagcccgtg cgaggcgtgt tgttccatcc cgacggcgcc
     1741 gaggtctatt ccagcgggag cgacaataag gtccatcgct ggaaaatcgc cgacgccgcc
     1801 aaatcggcgg aaatcggctt cggcggcgag gtctataagc tgcaaggcgc gggcgagttt
     1861 ttcgtgactg cctccgccga gaataaggtc cgccagttca aggccaagga tcaggcccag
     1921 gtccgcgagt tcgccggacc aaaggactgg gtgctctccg ccgcaatgca cgagggcacc
     1981 aagcgcgtgg cgggaggcac gtttgacggc caggtctttg tctggagcct ggatgacggc
     2041 gcggtggtca agtcgtggtt tgctgccccg ggctacacgc cgccgcagta ggcgcgcggc
     2101 gcatcttgca ctatgaatag ccgggatcaa acttggtccc ggagtgccac cagtggatcg
     2161 tccattcgca cttctccgag agcaagtttg ctctcggcta agggcagcat tatgcaaggt
     2221 ctctacgaaa aggtcgaagc agcggcccag acgattcgct cggtctggaa aacctcgccg
     2281 catgccggcg tcatcctggg cactgggctg ggcggcctga cgcagcacat caaagaggaa
     2341 gcctctcttg aatacgagga gattccgggc tttccgaaga gcacggcgac gagccaccgg
     2401 gggcggctgg tgtgcggcaa gctgggcgag ttgccggtgg tggcaatgga aggccggctg
     2461 cacatgtacg agggctacga cttgaatcag gtcacgctgc cggtgcgggt gatgaaggcc
     2521 atcggcgcga agctcttggt cgtcagcaac gcctgcggcg gaatgaatcc gctctatgcc
     2581 ctgggcgaca tcatggtcat cgacgaccac atcaacctga tgggggccaa tccgctgatc
     2641 ggcatcaatg acgaccgcct ggggccgcgg ttccccgaca tgagccagcc ctacgactgc
     2701 aagctgatcg accgggccct ggaaatcgcc cgccgcgaga acatcgtcgc ccaccgcggc
     2761 gtcttcgtcg ccgtcagcgg gccgaacctg gaaacgcggg ccgagtatcg cttcctgcgg
     2821 atgatcgggg cggatgtcgt cggcatgtcc acggtgccgg aggtgattgt cgccgcccac
     2881 gcagggctga aggtcgtcgg tttctcgatc gtgaccgaca tgtgcctccc cgatgccctg
     2941 gagcccgccg acgtgcccaa gatcatcgcc accgcgaatg ccgcggagcc gaagctgacg
     3001 aaacttgttt tgggcgtact cgcaggtgag agcgtctagg cgggcaagcc caatgtccaa
     3061 agtccaaggt ccaaagttgt tgtcttccga ccttggactt tggactttgg atcttggacc
     3121 tgccctggcc gccatccgtc gccactggaa gggccaagcg gcgaccggcg tcatcctggg
     3181 caccggcctc ggcgaaattg ccgaagaagt tgcggccgat gcgatcatcc cctatcgcga
     3241 gattcccggg ttcccgtact ccaccgcgat ggcccacaag ggccggctgg tctgcggcct
     3301 attgaacggc acgccggtcg tgatgctcca gggccgctgc cacctttacg aaggctattc
     3361 gttggcagcc gtcacgtttc ccacgcgcgt gctgcacgcc ctgggcatcc agacgctgat
     3421 cgtcaccaac gctgccggcg ggatcaatcc gcaatacgcc gtcggcgacg tgatgctgat
     3481 cgacgaccac attaacctga tgtggctacc ttccgtgggg caggcttcca gcctgccaaa
     3541 gatccgacac atcgcagccg cgaatctcta cgacgacgaa ctagctcatc tttcgctcgc
     3601 cattgcccgc cgcgccaact tcctcctcca ccgcggcgtt tatgtcgctg tcaccggccc
     3661 cagttacgaa acgcgcgccg agtaccgcgc attccgccga atcggcggcg actgcgtcgg
     3721 catgtccacc gttcccgaag tgctgaccgc cgccagcctc ggcctgcgcg tgctcggcct
     3781 ttcgacgatc acgaatgtcg ctcgcccgga cgctccgcaa actgtttccg ccgaagaggt
     3841 cgtccacgtc gccgccagcg ccctgccgaa ggtcagggcg atcattcacg gcatgctgaa
     3901 ccacatgcac cagacgggag ctaacagcct caggaatccg gcggcaagtg tgccgccggc
     3961 tgttgcgaac agcgactgac taccgccgcc tgtccgccgt gaccatcggg aagctatccg
     4021 tccgaaacgg cgaagcaggc agtccagcgc cgttgaacag gttgggctcc gaggtatcgg
     4081 tccaaccgaa gcggacggcg acgggctttg cgacgtcgga tgagctgacg acgatcgttt
     4141 cgccgtcgat ctcggccttt gccgggacga acttcatgtc ctcgccggcg atcgtaaagt
     4201 gctcgcccgg cttgctgggg tcgaacttca ggccggccgc gtgcttgaat ttgatgcgaa
     4261 tcttgttgcc ctcaatcgcc atcgaatcgt acagcgggcc ggaatagtcc agatcgctct
     4321 tgccgtacga tcccgccaga gcccacagcg ccaggcgagc gccaacatcc tgcttgttcg
     4381 gcggatggat gttgcggatc gtggcaatgt cggtcgtgac ggccatgccc gtgttggggc
     4441 tggcggtcag cgtcttgagc tgcgcctccc acagctcagg aagctggccc gtatccttca
     4501 tgccagcgcg atcgtagatg tacggcgcga gctgcacgaa gagaaacgga aaatcgccct
     4561 gaccgaaatg cttccgccag tcggcgatca tcgcgggaaa cagcttcgcg tactgctcag
     4621 cccggctgac gttcgattcg ccctggtacc agatcgcccc cttgatcgca aacgggatca
     4681 gcggttggat catgctgttg taaaggacac cggcctgatt cggattgcct ggcttgaact
     4741 gcttgtcgcg ctcgaggatc ggaccgaacc actcggggtc gctagcgagc gtctcgtggc
     4801 tggtccaggc ttcggcgagc gtgccgcccc aattggtgct gatcatgccg atgggcacct
     4861 tcaactcctg gttgagcttg cgagcgaaga acaggccgac cgccgacatc ggcgccgccg
     4921 tctcgggcga acagacggcc cagccgctgg ttgcgccctt cgggttggga acgaattcaa
     4981 agtcttccag cggcttgtcg gatgggttgc ggccgacttt gatcatccgg atttgcgggt
     5041 agttggcgtt cttgatttct tcggcggcat tcgtcgactg ggcaaccgac cactccatgt
     5101 tcgactggcc tgaggcaatc cagacttcgc cggccagcac gtcggtcagc ttgagttcgt
     5161 tcgtgccttt aaccaccaac tccaacgcag tctcactagc tgcgggagtc ttgatcttca
     5221 ccatccactt gccgtcggcg ccggccttgg tggaagcttt tgcttcgcca agcgtcacgg
     5281 tcacttcctc acccgcatcg gcccagcccc aaaccggggc ttcggtctgc tgctggagga
     5341 ccatgtggtc gccgaagatt ttggggagcc gaacgtcggc ccgggcactt ccgacgatca
     5401 caatcgtcgc gagggcagcc aggaacaggg gcgagcgtct catgcggatg aactccaggg
     5461 gggcggtctg ggaatgccaa tgttgggctg attctacttg ccgcgagatg ggcgtgctag
     5521 gagcttcgag ccctacgacc tagcccagca cctgcagaat gccccggcag aaatcgggca
     5581 ggtcgtccgg cttgcggctg ctgacgaagt ggcggtcgac gacgacggcg gcgtcttccc
     5641 agacggcgcc ggcgttgacc aggtcgtcct tgatgcccag cgagccggtg acgcggacgc
     5701 cgcgatagac cccggccgag atcggaatcc agccgccgtg gcagatggcg gcgatcaact
     5761 tgccggcaac gtcgaaatcg cgcacgagct gcagcacgtc cggatcgcgg cggagcttat
     5821 cgggcatgaa gccgccgggc acgaccaggc cgtcgaagtc ggccgcccgc atctccacga
     5881 tcgccgcgtc ggccacgcac ggatagccgt tcttaccctc gtatttctcc cccgattttt
     5941 cgcccgccac ggtcacggct gctccggcct cgatcagccg cagcttcgga taccacagct
     6001 ccaggtcttc gtagctgtcg ccgacgaagg tgaggatgcg cttgccaatg agtggctgct
     6061 tggcggccat ggtggttctc ctgcgtagat tgtcgtcgtt cgctccgcga acgaacgccc
     6121 ccagcgcgga gcgaaaggcg acaatggttc tttgtaacgc gccggtgggg tgcttg
//
LOCUS       DASUGB010000864         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1345765, whole genome shotgun sequence.
ACCESSION   DASUGB010000864 DASUGB010000000
VERSION     DASUGB010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345765"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2178
                     /locus_tag="VFV87_16415"
     CDS             <1..2178
                     /locus_tag="VFV87_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX5105405.1"
                     /translation="GKYDAAITFFHEATISALYFDRFDVMEEGFRLGALTHSVSGQPG
                     VYPPLVPAAVASKRVRMVQTSILAALAEQLCTAGDLADAAQTIGQARANLNRREMALG
                     AIGSRVNYQAARIALQSGDARTAGSALNTALTYQKGSSKRLFQIALSDRLFTTGEVTE
                     RVADLLYSDVLREPIRTDWIVDPLDTLAVGMSAHPQPYEHWFELALSRKEIEKAIDIG
                     EQIRRHRYFATQALGARLLALRWVLEAPPEVLSKEAALQRQDLLLKYPKFAELSQQAK
                     KIRDELLALPVDPEDDAGKQQQETLLADLAKTSAAQEALLQIMALERVPAEFAFPPLR
                     ETKELQAQLPEGTLVFYYLATSRNVHAFAISNQRYGHFVVAQPAKVKADISEMLRSQG
                     HHDRTQPVGVEDLADEAWKVPAERLLGQLTNNTQPEEWAKYSELVIVPDSVLWYLPFE
                     ALPVKLGEETMPLLTQLPIRYAPTLSLAVPDRRGNRPLPRTALIAGKLLPRDSDQAAN
                     ALRDTVVDAIPGTAVLGDPLPATSGTLAATFDRLVVLADIDDGKLPFGWAPLVLDAGK
                     PGSSLAEWSTLPWAGAEQIILPGFHTPAEYGLKKGGTGEEVFLSVCGLMASGSRTVLL
                     SRWRVGGQSTANLMREFVQELPHEGAAAAWRRSVQLARESLLDPALEGRLKPSQAADG
                     LKADHPFFWSGYLLVDTGLTPVKEGAIPVAEGGAVPGAKPAGE"
     gene            complement(2213..3178)
                     /locus_tag="VFV87_16420"
     CDS             complement(2213..3178)
                     /locus_tag="VFV87_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5105406.1"
                     /translation="MQRSTAMAVVVFYLQVTLALQAAEDAVQPEQADGEAPITLKGHR
                     DGVFSVRFVRGGKVIASASRDGTIRLFDVQSGEQAAELRGHEGQVLRLALGPENLLAS
                     AGADKTVRLWDLQEQKEVARLEGHEEWVAGVAFSPNGGLLASAAADKSVKLWDVAKRS
                     LVREFTSNAAAYSVCFSPDGKHLASAFNNGTIKVWEVDSGRELLSLKAHDKEIHTLIY
                     APDGQRLATASADGTIKILRVEDGKELCSLAGHDGPVYTIAFCSADRIVSSGGDRTVR
                     LWDAASGEELQLWEGHKNEVFAVAWNAATRQFASGSQDRTVRIWK"
BASE COUNT          554 a         1083 c         1037 g          557 t
ORIGIN      
        1 ggcaagtacg acgcggcgat cacgttcttc cacgaggcga cgatcagcgc cttgtatttc
       61 gaccggttcg acgtgatgga ggagggtttc cggctggggg cgctgactca ttcagtcagc
      121 ggccagccgg gagtgtatcc gccgctggtt cccgcggcgg tggcgtcaaa gcgggtgcgg
      181 atggtgcaga cgtcgatcct ggcggccctg gccgagcagc tttgcacggc gggcgatctg
      241 gccgacgccg cgcagacgat cggccaggcc cgggcgaacc tcaatcgccg cgaaatggcc
      301 ctcggcgcca tcggcagccg ggtgaattac caggcggccc gcatcgccct gcaatcgggc
      361 gacgcacgga cggccggctc ggcgttgaac accgcactga cctatcagaa gggctcctcg
      421 aagcggctct ttcagatcgc cctgtccgac cggctgttca cgacgggcga agtgaccgag
      481 cgcgtggccg atttgctcta cagcgacgtc ctccgcgagc cgatccgcac cgactggatc
      541 gtcgatccgc tcgacacgct ggcggtcggc atgtcggccc acccgcagcc gtacgaacac
      601 tggttcgagc tggccctgtc gcgaaaggag atcgagaagg cgatcgacat cggcgagcag
      661 atccgccggc atcgctactt tgcaacgcag gcgctcggcg cacggctgct ggctttgcga
      721 tgggtgctgg aggcgccgcc cgaggtactg agcaaggagg ccgcgctcca gcggcaggac
      781 ttgctcctga agtatcccaa attcgccgag ctctcgcagc aagccaagaa gatccgcgat
      841 gagctgctgg ccctgcctgt cgatcctgag gacgacgcgg gcaagcagca gcaagagact
      901 ctgctggccg acctggcgaa gaccagcgcg gctcaagagg ccctgctgca aatcatggcc
      961 ctggagcgcg tgcctgcgga gttcgccttt ccgccgctgc gcgagacaaa ggagctgcag
     1021 gcgcagcttc ccgaagggac gctcgtcttc tattacctgg ctacctcgcg gaacgtacac
     1081 gcttttgcga tcagcaatca gcggtacggg cactttgtcg tcgcccagcc ggcgaaggtg
     1141 aaggccgaca tcagcgagat gctccgctcg cagggccatc acgaccgcac gcagccggtg
     1201 ggcgtcgagg acctggccga cgaggcgtgg aaggttcctg ccgagcgcct gctcgggcag
     1261 ctcacgaaca acacgcagcc cgaggagtgg gcgaagtaca gcgagctggt gatcgtgccc
     1321 gatagcgtgc tctggtatct gccattcgaa gcgctgccgg tcaagctggg cgaggaaacc
     1381 atgccgctct tgacgcagct tccgattcgg tacgcgccga cgctctcgct ggccgtgccg
     1441 gaccgccgcg gcaaccgtcc gctgccgcgg accgccctga tcgcggggaa gctgctgccg
     1501 cgcgacagcg accaggccgc caatgcgctg cgcgatacgg ttgtcgatgc cattccgggc
     1561 acggcggtgc tcggcgaccc cttgccggcc acctccggca cgctcgcggc gacgttcgac
     1621 cgcctggtcg tcctggcgga catcgacgac ggcaagctcc ccttcggctg ggcgccgctg
     1681 gtgctcgacg ccggcaagcc gggcagctcg ctggccgaat ggagcacgct gccgtgggcc
     1741 ggggccgagc agatcatcct gcccggcttt cacactccgg ccgaatatgg cctgaagaaa
     1801 ggcggcaccg gcgaagaagt cttcctctcg gtctgcggct tgatggccag cggcagccgc
     1861 acggttttgc tcagccgctg gcgggtcggc gggcagagca cggccaacct gatgcgggaa
     1921 tttgtgcagg agctgccgca tgaaggcgcg gccgcggcct ggcggcgcag cgtgcagctt
     1981 gctcgtgaaa gcctgctcga cccggcgctc gaaggccgcc tcaagccttc ccaagccgcc
     2041 gacggtctga aggccgacca cccctttttc tggtccggct atttgctggt cgatacggga
     2101 ctgacgccgg tgaaggaagg tgcaattccg gtcgcggagg gtggcgctgt acccggggcg
     2161 aagccagccg gcgaatagag aacgcggacg ccaccaaact cgtcaagttc ggctatttcc
     2221 aaatccgcac ggtccggtcc tggctgccgg aggcgaactg tcgcgtcgcg gcgttccagg
     2281 cgacggcgaa aacttcgttc ttgtggcctt cccagagctg aagctcttcg ccgctggcgg
     2341 catcccaaag acgaaccgtc cggtcgcctc ctgaggagac gatgcggtcg gcgctgcaaa
     2401 aagcgatggt gtaaacaggg ccgtcgtgtc ctgccaagct gcacagctcc ttgccgtcct
     2461 cgacacggag gatcttgatc gtgccgtcgg cactagccgt ggccaggcgc tggccgtccg
     2521 gagcataaat gagcgtgtgg atttccttgt cgtgcgcctt gagcgacagc agctcgcggc
     2581 cgctatccac ctcccaaacc ttgattgtgc cgttattgaa ggcgctggcc agatgcttgc
     2641 cgtcaggact aaaacagacc gaataagccg ctgcattgct cgtgaattcc cggaccaggc
     2701 tgcgcttggc gacgtcccac agcttgacgc ttttatccgc agccgccgag gccagcagcc
     2761 cgccattcgg gctgaaggcc acgcctgcaa cccactcctc gtgtccttcc agcctggcga
     2821 cttccttttg ctcctgcagg tcccacaagc gcaccgtctt atccgccccg gccgatgcca
     2881 gaaggttctc cggtcctaat gccaggcgca gcacctgccc ttcgtgtcct cgtagctcgg
     2941 cggcttgctc gcccgactgc acgtcaaaca agcggattgt tccgtcgcgc gaagccgacg
     3001 cgatcacctt cccgccgcgt acaaacctca cgctgaacac tccatcgcgg tgccccttga
     3061 gtgtgattgg cgcctcgccg tcggcttgct ccggctgcac ggcatcctcc gccgcctgca
     3121 gcgccagagt gacctgcaag taaaacacta ctactgccat cgctgtgctg cgctgcatac
     3181 gatcctcacc tttgcgaaca ctcggaagcg aatggattca cagcggcatt t
//
LOCUS       DASUGB010000865         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2041305, whole genome shotgun sequence.
ACCESSION   DASUGB010000865 DASUGB010000000
VERSION     DASUGB010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041305"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFV87_16425"
     CDS             <1..517
                     /locus_tag="VFV87_16425"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5105407.1"
                     /translation="AAQGVLMFARRIGRERGERAVRLAGQAIRGVALGVIVTALVQSL
                     FAGLGLWVSGVPRPGILLAVIFVLGIAQIGPLPVLLPAVIWLYWSGSAGWASALLVWT
                     VLVGALDNFLRPVLIRRGVDLPLLLIIPGVIGGLISFGVVGLFIGPVVLAVTYTLLES
                     WIREDKGTAVT"
     gene            complement(816..>2152)
                     /locus_tag="VFV87_16430"
     CDS             complement(816..>2152)
                     /locus_tag="VFV87_16430"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX5105408.1"
                     /translation="GEGAAMVDVLGWRLDDTVALIRGAADSVVVLDVLPADAGPDGKH
                     KLVSLVRKTISLEQQAAKASVHSIVDGKVTRRVGVITLASFYEDFAARQRGVKDYRSA
                     ARDVARLLEGLKREKIDSVLIDLRNNGGGSLAGAVELTGLFIDVGPVVQQRNANGQIS
                     VESDTRAGVAWDGPLGVLINRGSASASEIFAAAIQDYGRGLVIGEPSFGKGTVQSVVD
                     LDRIAKNSKPQYGELKMTVAQFFRINGGTTQLRGVTPDILFPAVSDAESFGESSFANA
                     LPWTQIKPADYVQAGDLKGLLPVLLALHDARVKNDKDFQYLQEDIAESRLRRKKNLIS
                     LNEAERRKERAAEEARLMARETRKDPSKNSSNDVAGKDTASDKGAALRDDGLQPGERK
                     LANELAAEKARKDAKDVLLYEAVNILGDEVAVMKTGGGLTAGIQRGPVLLLE"
BASE COUNT          344 a          691 c          658 g          459 t
ORIGIN      
        1 ggccgcacaa ggtgtactca tgttcgcccg acgcatcggg cgggagcgtg gtgagcgggc
       61 ggttcgtcta gccgggcaag ccattcgcgg tgtggccctg ggagtgatcg tcactgcgct
      121 ggttcagtcg ctgttcgccg gtctcggtct ttgggtgagc ggcgtgccgc gcccgggcat
      181 cttgctggcc gtcatttttg tgctcggcat cgctcagatc ggaccgctgc cggttctgct
      241 gcccgcggtg atctggcttt actggtcggg aagcgccgga tgggcgagcg cacttctggt
      301 atggaccgtg ttggtcggag cgctggacaa cttcttgcgg ccggtactga ttcgtcgcgg
      361 cgtggatctc ccgttactgc tcatcattcc gggggtgatc ggcgggctca tcagtttcgg
      421 ggtcgttgga ttgttcattg gccccgtggt cctcgccgtc acctatacgc tgctcgagtc
      481 ctggatccgg gaagataaag gaacggcagt gacgtaaacc gacgcctcat agcgtccgtt
      541 tattcgagcg acggcagaag ggaggcgtcc gtgggcggga gagggcggca attccgcgcg
      601 agtcggagac ctttcttcaa gagcagacgg tcgaagtacc tcgcctacgt gacgtgcagc
      661 ggcggcgaat ccgaccatcg ggccgtcgcc gctgcgccgc gtgattccct gcatggcgcc
      721 agcgattccc aggccatcga gcgcgtcgga ccacgtgctg ctgcccatgc gagcgcgccc
      781 tcgcttggtg gcggcagcca gtcctgcgcg ggtctctatt ccaacagcaa cacggggccg
      841 cgttggatgc cggctgtgag cccgccgccg gttttcatca ccgctacttc atcgcccagt
      901 atattgacgg cttcgtataa caggacgtct ttcgcgtcct tcctcgcctt ttcggccgcc
      961 agctcgttgg cgagcttgcg ttcgccaggc tgcaggccat cgtctcgaag cgcggccccc
     1021 ttatcggacg cggtgtcctt gcctgccacg tcgttgctgg aattcttgct ggggtctttt
     1081 cgcgtctcgc gagccatgag tcgagcctct tcggctgccc gttctttgcg acgctcggct
     1141 tcgttcagcg agatgaggtt cttcttgcgc cgaagcctgg attctgcgat gtcttcttgc
     1201 aggtactgga agtctttgtc gttcttcacg cgcgcgtcgt gcagtgccag gagaaccggc
     1261 agcaagcctt tcaagtcgcc ggcctgcaca tagtccgccg gcttgatctg cgtccagggg
     1321 agcgcattgg cgaagctgga ctcgccaaag ctctccgcgt cggaaaccgc aggaaagagg
     1381 atatccggag tcacgccgcg caactgcgtt gtaccgccat tgatgcgaaa gaactgcgcc
     1441 acggtcatct tcaactcacc gtactgcggc ttgctgttct tggcgattcg atccagatcg
     1501 accacgcttt gcacggtacc tttgccaaag ctcggctcgc cgataaccaa cccgcggccg
     1561 taatcctgaa tcgccgcggc aaagatctcc gacgccgacg cagaaccgcg gttgatcaaa
     1621 acgcccagag gaccgtccca ggcaacaccg gctcgcgtat cgctctccac tgagatctga
     1681 ccgtttgcgt tacgctgctg caccaccggc cccacgtcga tgaatagacc ggtcagctcg
     1741 accgctcccg ccagcgagcc gccgccgttg ttgcgcaagt cgatcagtac gctgtcgatc
     1801 ttttccctct tcagcccttc cagcaagcgg gccacatcgc gggccgcgct cctgtaatcc
     1861 ttgacgcctc tttgtcgggc tgcaaaatcc tcatagaacg aagcaagggt tatcacaccg
     1921 acgcgacgcg tgaccttccc gtcaacgatg gagtgcacgg atgctttggc ggcctgctgc
     1981 tccagactga tcgtcttgcg cacgagagaa accagtttgt gtttgccatc ggggccggcg
     2041 tccgccggga gcacgtcgag cacgaccacc gagtccgctg caccgcgaat cagcgcaaca
     2101 gtgtcgtcga ggcgccagcc caagacgtcc accatcgcgg cgccttcgcc ct
//
LOCUS       DASUGB010000866         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_930339, whole genome shotgun sequence.
ACCESSION   DASUGB010000866 DASUGB010000000
VERSION     DASUGB010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930339"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV87_16435"
     CDS             <1..342
                     /locus_tag="VFV87_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105409.1"
                     /translation="NITPYRNPLAGRTYDPGKRTSEYIDEWTQFTFDPASLGDIEQHF
                     GFPFCYREVGYTDGKQVDLFYGAEMHFWQHKLAEDLWIALAAVLACGIATEWLSRRCV
                     PRGRESIAAQS"
     gene            complement(408..1073)
                     /locus_tag="VFV87_16440"
     CDS             complement(408..1073)
                     /locus_tag="VFV87_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105410.1"
                     /translation="MRTSSACWAMGGLLVASSSVFGQAQVPTTVQLPTFRFFTVQTSV
                     SVPDGGGTYLGGMKSARDSSLTRGFGPFRNRGIGSDRGAAGMSVHATITDLKEMDDAI
                     LAEAAAGRPGAAAFDPDAARAELLSRHVGKTFTSASPAEASEPAPLPGSVAAIRAQNA
                     AAAEARAAEAAQYFAKAQTAEGEGKPAVAKYYYQMVARRDKGALQQQALERIAAIDGA
                     KGR"
     gene            1253..1876
                     /locus_tag="VFV87_16445"
     CDS             1253..1876
                     /locus_tag="VFV87_16445"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5105411.1"
                     /translation="MSTVLPPTTPFSFGPPDMPPEPVLRLSVDQYLEMVAKGIIQEND
                     PVELLEGWLVPKMTKNPDHWICATLIRDALIRLSIAGFFVHSQDPVRTSDSVPEPDVA
                     LVRGKPRDYRGGNPQPENLALVVEVADTSLRRDRAIKKRVYARAGVPVYWIVNLKDSE
                     IEVYGEPTGPGKSPTYKAMQQIGADGEIEVVVDGREVGRLKVKDLLP"
     gene            2020..2292
                     /locus_tag="VFV87_16450"
     CDS             2020..2292
                     /locus_tag="VFV87_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105412.1"
                     /translation="MIAVAGLSVAVTDERSNYFQRGTWRDSAIAVGKVLTLAAPLAIP
                     MAFASPVIILVFLRRWANGDWRYGIAAAAELAIAYANWCALLPAVQ"
     gene            2334..>3239
                     /locus_tag="VFV87_16455"
     CDS             2334..>3239
                     /locus_tag="VFV87_16455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5105413.1"
                     /translation="MVRCSGLAVFLSLLSMQALADEPAPFQQPAISVPEGYSVELAAA
                     PPLVTHPIMAGFDERGRLYVAETAGHNMQRAELEEKLPNFVTRLEDTDGDGKFDKRTT
                     FADKLTFPQGALWHEGWLYVASSGAIWRFRDKDDDGVAEIREKIVSSFGYTGNAADVH
                     GCFLGPEGRIWWCEGRHGHDIVDAAGQSVSKGKAARIFSCRLDGSDVQIHCGGGMDNP
                     VEIDWTDEGEVLGTVNIFYHKRGDCLVHWMHGGVYPRHDQPQIVGEFRRTGDLLDAVH
                     DYGHVAVSGTCRLRSESFLDLLPSHE"
BASE COUNT          568 a         1011 c         1039 g          621 t
ORIGIN      
        1 aatatcacgc cttatcgaaa tcccttagcc ggacgcacct acgatccggg aaaaaggacg
       61 tccgagtata tcgacgagtg gacgcaattc actttcgacc ccgcgtccct tggcgacatc
      121 gagcagcact tcggctttcc gttctgctac cgcgaagtgg gctacacgga cggaaagcaa
      181 gtcgatctct tctacggggc ggagatgcac ttctggcagc acaagctggc ggaagacctg
      241 tggattgccc tggcggcggt gctcgcctgt gggatcgcga ccgagtggct cagccggcgc
      301 tgcgtgccac ggggtcggga gtcaattgcg gctcaaagtt aaccatatgg atggtcgtga
      361 ccgcaattga ctcccgaccc ctgcccgcct gcgattcgcg gcaacgccta tcgtcccttc
      421 gccccatcaa tcgccgcgat tcgctctaat gcctgctgct gcagggcccc tttgtcgcgg
      481 cgggcgacca tttgatagta gtacttggcc accgccggct tcccttcgcc ttccgcggtc
      541 tgggctttgg cgaaatactg ggccgcttcg gcggcgcggg cctcggctgc ggcggcattc
      601 tgggcgcgaa tggccgccac gctgccggga agcggcgcgg gctcgctggc ttccgccggc
      661 gaagcgctcg tgaatgtctt gcccacgtgc cgcgaaagca gctccgcccg ggccgcatcg
      721 ggatcgaacg cagcggcccc cggcctgccg gcagccgctt cggccaggat cgcgtcgtcc
      781 atctccttca ggtcggtgat cgtcgcgtgg acgctcatcc cggccgcccc gcggtcgctg
      841 ccgatccccc ggttgcgaaa aggcccaaat ccccgcgtca agctgctatc gcgtgcggac
      901 ttcatacccc ccagatacgt cccgccgcca tccggcactg agaccgacgt ctgcaccgtg
      961 aagaaccgga acgtcggcaa ctgcaccgtc gtcggcacct gagcctggcc aaagacgctt
     1021 gaactcgcaa caagcagccc gcccatcgcc caacacgcag atgaagtccg catcgctcgc
     1081 ctccttgatc tgacaaccgt gattgtagcg aatctcggcc cgcaagctca acaatccggc
     1141 gcggcaatgt tcgatttcgc cgcgttcgaa ttcgattgtt gacgctgctt gccgcggggg
     1201 ctaccgtgag acaatgcctt cagggcaaaa cttcttcgcg gtgagtagcg cagtgtccac
     1261 agttcttcct ccgacgacgc cgttcagctt cggaccgccc gatatgccgc ccgagccggt
     1321 attgcgcctc agcgtcgatc agtatctgga gatggttgcc aaaggcatca tccaggagaa
     1381 cgatccggtc gagttgctgg aaggctggct ggtgccaaag atgaccaaga atcccgatca
     1441 ctggatttgc gccacgctga tccgcgacgc cttgattcgg ttgtcgatcg cgggtttctt
     1501 tgtccattcg caagatcccg tccgaacgtc ggatagcgtc cccgagcccg atgtcgctct
     1561 cgtgcgcggg aaaccgcgcg attatcgcgg cggcaatcct cagccggaga acctggcact
     1621 cgtcgtcgag gtggccgata cgtcgctgcg ccgcgaccgc gcgatcaaga agcgggtgta
     1681 tgctcgcgcg ggagtgcccg tgtattggat cgtgaatctc aaggacagcg agatcgaagt
     1741 gtatggcgag ccgaccggac cgggaaagtc gcccacttac aaggcgatgc agcagatcgg
     1801 cgcggatggc gagatcgaag tggtcgttga cgggcgcgaa gtgggccggc tgaaggtcaa
     1861 ggatttgctg ccgtgaatgg caatgcgaac cagatgacga cggacgcaat cgctggttct
     1921 gcctcaagct gcaatgcttc ggaacagacg ttgccgccgg tcgcgatcag acgccagcaa
     1981 ttctggtatt cctggtggcg cgtgtatctg ttcgtccatt tgatcgccgt cgccggactg
     2041 agtgttgcag taacagacga gcggtcgaat tattttcagc gcggtacctg gcgcgattcg
     2101 gccattgcgg tcggcaaggt cctcactctc gcggctccac tcgcgattcc gatggcgttc
     2161 gcatcgcctg tcatcattct cgttttcctg aggcgctggg cgaacggcga ttggcgatat
     2221 gggatcgccg ctgccgccga attggcgatc gcctatgcaa actggtgcgc gttgctccct
     2281 gcggttcagt agaatggcag tcgcatttct tctggcctgc agggagatct agtatggtcc
     2341 gctgctccgg tttggctgtg tttctttcgc tgctgtcgat gcaggctctt gccgacgagc
     2401 cggcaccctt ccagcagccc gcgatctccg tgccggaggg ctactccgtt gaactcgccg
     2461 cggcgccgcc gttggtgacg catccgatca tggcggggtt cgatgagcgg ggacggctgt
     2521 atgtggccga gaccgcgggg cacaacatgc agcgggcgga gctggaggag aagctgccga
     2581 atttcgtcac gcggctggaa gataccgacg gcgacggcaa gttcgacaag cggacgacgt
     2641 tcgccgacaa gctgacgttc ccgcaggggg cgctgtggca cgagggctgg ctgtatgtcg
     2701 cttccagcgg ggcgatctgg cggtttcgcg acaaggacga cgacggcgtc gccgagattc
     2761 gcgagaaaat cgtcagcagc ttcggctaca ccggcaacgc ggccgacgtc cacggctgct
     2821 ttctcggccc cgaggggcgc atctggtggt gcgaagggcg gcacgggcac gacatcgtcg
     2881 atgcggccgg ccagagcgtc agcaagggga aggcggcccg catcttctcg tgccgcctcg
     2941 acggcagcga cgtgcagatt cactgcggcg gcggcatgga caacccggtc gagatcgact
     3001 ggaccgacga aggggaggtg ctgggcacgg tcaacatttt ctatcacaag cggggcgact
     3061 gcctcgtaca ctggatgcac ggcggcgtct atccgcggca cgaccagccg caaatcgtcg
     3121 gcgagttccg ccgcacgggg gacctgctcg acgcggtaca cgattatggg cacgtcgcgg
     3181 tcagcggcac ctgccggctg cggagcgagt catttctgga cttgttgccc agtcatgag
//
LOCUS       DASUGB010000867         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_235875, whole genome shotgun sequence.
ACCESSION   DASUGB010000867 DASUGB010000000
VERSION     DASUGB010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235875"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..998
                     /gene="nadC"
                     /locus_tag="VFV87_16460"
     CDS             54..998
                     /gene="nadC"
                     /locus_tag="VFV87_16460"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5105414.1"
                     /translation="MSKEFRQLAWSSDLEEDCRRIVRLAVYEDLDRGYDWTTICLVPE
                     EAQAAASVVVRQPGVIAGLDAAPVVLDEMDIAAEFQLQSQDGDVVEAGTVVATIAGPA
                     RDLLTSERALLNLIGRLSGIATLTRKYVDAVAGTKARIYDTRKTAPGWRRLEKYAVRC
                     GGGHNHRTGLYDAILIKDNHLAFGREQSGETSFSPSAAVRRAKAFLAEQARGKTPFPG
                     SANPEMIVEIEVDSLDQLREVLPEKPDIVLLDNMSLDQLCEAVSIRDGGRWLSELEAS
                     GGVSLQTVRAIAETGVERISAGALTHSAVSLDVALDWK"
     gene            complement(1025..1489)
                     /locus_tag="VFV87_16465"
     CDS             complement(1025..1489)
                     /locus_tag="VFV87_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5105415.1"
                     /translation="MYGELVPVGGGDTIPLLRPSLMVGRRESCDICLRFDNISGQHCQ
                     LSLDSGYWFVQDLNSQNGTKVNGHRVVRKRLDPGDTLAIARHKYTIQYSPVALGASGP
                     PPSDDEALETVLGKSLLDKAGLQRRPGSDGKRYDVTNDQAGQLKNRKRSSDD"
     gene            1674..>2401
                     /locus_tag="VFV87_16470"
     CDS             1674..>2401
                     /locus_tag="VFV87_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912563.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEX5105416.1"
                     /translation="MQTRALSLSESSVAAGEQPVWRRAGLRYYAYNFFLRQKFGRRVQ
                     KVSLDAGFTCPNVDGTVAIGGCTFCDNRSFSPSRRVRRQDIWDQLEDGMRRLKRKYDD
                     VDRFIAYFQPATNTYGPVERLRELFEQAIEHPKVVGLAIGTRPDCVPDEVLGLLQELA
                     GRTYLSVEYGLQTIHDRSLDWMNRGHHHDATVDAVTRSRGRGFEICGHVILGLPGESH
                     ANMLATAREVARLQLDAVKIHNLYA"
BASE COUNT          470 a          746 c          747 g          438 t
ORIGIN      
        1 atttgaaatc tgatatttga aatgtgaaat agtgctagcg tcgaaaggtc ccaatgagca
       61 aagaattccg ccaacttgcc tggtccagcg acctcgaaga ggactgccgg cggattgtgc
      121 gcctagcggt ctatgaggac ctggaccgcg gctacgactg gacgaccatc tgccttgttc
      181 ccgaggaggc gcaggcggct gctagcgttg tcgtccggca gccgggtgtg atcgccggct
      241 tggacgcggc gccggtcgtg ctggacgaaa tggatatcgc ggctgaattc cagttgcaat
      301 cgcaagacgg cgacgtggtg gaggcgggga cggttgttgc gacgatcgcc ggccctgcgc
      361 gcgacctatt aaccagcgag cgggcgctgc tcaatctcat cggccggttg tcaggtatcg
      421 ccacgctcac gcggaagtac gtggacgccg tcgccgggac aaaggcgcgt atttacgaca
      481 cgcgcaaaac cgctcccggc tggcggcggc tcgagaaata cgcggtccgc tgcggcggcg
      541 ggcataacca ccgcacgggg ctttacgacg cgatactcat caaggacaac cacctcgcct
      601 tcggccgcga gcaaagtgga gaaacctcgt tctcgccttc cgctgccgtg cggcgggcca
      661 aggcgtttct cgccgagcaa gcccggggca agacaccgtt tcccggcagc gccaatccgg
      721 aaatgatcgt cgaaatcgaa gtcgattcgc tggatcaact gcgcgaagtg ctgccggaga
      781 agcccgacat tgtcctgctc gacaacatgt cgctcgatca gctttgcgaa gcggtgtcga
      841 ttcgcgacgg aggccgctgg ctgagcgaat tggaggcgtc cggcggcgtc agcctgcaga
      901 cggtgcgagc aatcgccgaa acgggcgtcg agcggattag cgccggcgca ctcacgcact
      961 ccgccgtgag cctcgatgtg gcgctcgact ggaagtgacg gcacacggag tgtgcctgct
     1021 actttcaatc gtccgacgat cgcttgcgat tctttagttg cccagcctgg tcgttcgtca
     1081 catcgtagcg tttaccgtca gagccgggac gccgctgcag gccggctttg tcaagcaacg
     1141 acttgccgag caccgtttcg agcgcttcgt cgtcgctcgg cggtggtcca ctcgcgccca
     1201 gggccacggg cgaatactga atcgtgtatt tgtgccgggc aatcgccagc gtgtcgcccg
     1261 gatcgagccg cttgcggaca acgcggtggc cattcacctt cgtaccgttc tgactgttca
     1321 gatcctgcac aaaccaatag ccgctatcca gagaaagctg gcaatgctgg ccagagatat
     1381 tgtcaaaccg caagcaaatg tcgcacgact cgcgacgacc gaccatcagc gatggccgca
     1441 agagcggaat cgtatcgccc cctcccacgg ggaccaattc accatacata tcgctgaccc
     1501 ttgagcgggc cggcagccgg acctatcctg attgtcggac agatctcgcc tgaatgcccc
     1561 taacttctgt ttcggacgag atggctttta ctctacgcaa ggggccgacg atcgtcaacc
     1621 aaatccagta ctttcgggct gggcaagcgc cgcggcacgc agcgagcgag agtatgcaga
     1681 caagggcgct tagtctgagc gaatcgagcg tcgctgccgg cgagcaacct gtgtggcggc
     1741 gggccggcct gcgatactat gcatacaact tctttctgcg ccagaagttc ggtcggcgag
     1801 tgcaaaaggt gagcctggac gctggattca cctgcccgaa cgtcgatggc acggttgcca
     1861 tcggcggctg cacgttctgc gacaaccgca gcttcagccc cagccgccgt gtccgccggc
     1921 aggacatttg ggaccagctc gaagacggca tgcggcggct caagcggaag tatgacgacg
     1981 tagatcgctt catcgcgtat ttccagccgg cgacgaacac ctacggcccc gttgagcggc
     2041 tacgggagct gttcgagcag gcgatcgagc atccgaaagt tgtgggcctg gcgatcggga
     2101 cgcggccgga ctgtgtgccc gacgaagtgc tcggcctgct gcaagaactg gccggccgaa
     2161 cctatctttc cgtcgaatac ggcctccaga ccatccacga ccgctcgctc gactggatga
     2221 accgcggcca ccatcacgac gccacggtcg atgctgttac ccgcagccgc ggccgcgggt
     2281 tcgaaatttg cggccacgta attctgggcc tgcctggcga atcgcacgcc aacatgctcg
     2341 ccacggctcg cgaagtcgcc cggctgcagc tggacgcggt gaagattcac aacctctacg
     2401 c
//
LOCUS       DASUGB010000868         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_374687, whole genome shotgun sequence.
ACCESSION   DASUGB010000868 DASUGB010000000
VERSION     DASUGB010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374687"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="dxs"
                     /locus_tag="VFV87_16475"
     CDS             complement(<1..1383)
                     /gene="dxs"
                     /locus_tag="VFV87_16475"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5105417.1"
                     /translation="MHPLLSKLESAEGLQSMSVAELSQLGDEIRDVLCNLLQERTAHF
                     ASNLGVVELCLALHSVFDFRTDRLIWDTGHQIYPHKLVTGRYSRFKTMRTKGGLMGYP
                     NPHESPYDLFMTGHAGSSVSTISGLRSGEDLLGNTDRKTVAVIGDGAFPSGIVFEALN
                     NIGGMKQDVLVVLNDNKMSICPRVGGVASYLDRLRTSPFYSGLKSEVVRTLEKVPLLG
                     DPVERFLAQLKEAVKAGLHGGMMFEDLGFRYLGPIDGHNIAVLRKYLQMVKGLKGPVL
                     LHVVTEKGHGYVPAAADPVYFHTPPAFKQENGQPVPKKSGGGPAYTNLVSEAIRDELL
                     KNRRVTVLTAAMCQGNKLEKIREEFPNRFFDTGICESHAVAFAAGQAKAGLRPIVDIY
                     STFLQRSYDQIFQEVTLQNLPVVFTLDRAGLTGPDGPTHHGCFDIAYMRCLPNMVVMA
                     PGDAWDVPAML"
     gene            complement(1454..>2085)
                     /locus_tag="VFV87_16480"
     CDS             complement(1454..>2085)
                     /locus_tag="VFV87_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5105418.1"
                     /translation="DDLPAMDDDDMRRGRPSCHTKFGEATAILAGDALLAQAFEVLAT
                     GTEPPAVAARCVAELAKAAGACQLVGGQEDDLKAEFANLGLEQLEAIHRRKTGAMIRV
                     SLQLGGIVGQASEQQLAGLDRYGERLGLAFQIVDDLLDREGTAAALGKRTKKDHDHGK
                     LTFPALLGIEASRRRAEELVAGAIDAVSIFGPKADSLAALAKYVLERDH"
BASE COUNT          325 a          648 c          727 g          385 t
ORIGIN      
        1 gagcatggcg gggacgtccc aggcgtcgcc gggggccatc acgaccatgt tcggcaggca
       61 gcgcatatac gcgatgtcga agcagccgtg atgcgtcggg ccgtcgggcc cggtgagccc
      121 cgcgcggtcg agcgtgaaga cgaccggcag gttctgcagc gtgacctcct ggaaaatctg
      181 gtcgtacgac cgctgcagga acgtgctgta gatatcgacg atcggccgca ggccggcttt
      241 ggcctggccg gcggcaaagg cgacggcgtg cgactcgcag atgcccgtgt cgaagaaccg
      301 gttgggaaac tcctcacgga ttttctcgag cttgttcccc tggcacatcg ccgcggtcag
      361 caccgtcacg cggcggttct tcaaaagctc gtcgcgtatc gcctcgctga ccaggttcgt
      421 ataagccggg ccgccgccgg acttcttcgg caccggctgg ccattctctt gcttgaaggc
      481 gggaggagtg tggaaataaa ccgggtcggc cgccgccggg acgtagccgt gacccttttc
      541 ggtgacgacg tgcaggagga ccgggccctt cagcccctta accatctgca ggtatttgcg
      601 gaggacggcg atgttgtggc cgtcgatcgg cccgaggtag cgaaagccga ggtcttcgaa
      661 catcattccg ccgtgcaggc cggctttgac cgcttccttg agctgggcga ggaagcgttc
      721 gaccgggtcg cccaggagcg gcactttttc gagcgtgcgg acgacttcgg acttgaggcc
      781 ggaatagaac gggctggtgc ggaggcgatc gagatagctg gcgacgccgc cgacccgcgg
      841 gcagatcgac attttgttgt cgttcaggac gaccagcacg tcctgcttca tgccgccgat
      901 gttgttcagg gcctcgaaca cgatgccgct ggggaaggcg ccgtcgccga tgaccgcgac
      961 ggtcttgcgg tcggtgttgc ccagcaggtc ttcgccgctg cgcaggccgc tgatcgtgct
     1021 gaccgagctg ccggcgtggc cggtcataaa caggtcgtac gggctctcgt gcgggttggg
     1081 atagcccatc aggccgccct tcgtgcgcat tgtcttgaag cgcgagtagc ggccggtgac
     1141 gagcttgtgc gggtagatct ggtgccccgt gtcccagatc aggcggtcgg tgcggaagtc
     1201 gaacacgctg tgcagggcca ggcacagctc caccacgccc aggttcgagg cgaagtgagc
     1261 cgtccgctcc tggagcaggt tgcacagcac gtcgcggatt tcatcaccga gctgcgacag
     1321 ctcagcgacc gacatgctct gcaggccctc ggcggattcg agtttggaaa gcagcgggtg
     1381 catcggttca tcgtggggca ggcattcttg cctgcctaat ggtgcggcag gcaagaatgc
     1441 ccgccccacg ctcctaatgg tcacgctcca atacgtactt cgccagcgcc gccagcgaat
     1501 cggctttcgg gccgaaaatg ctgacggcat cgatcgcgcc ggcgaccaac tcttctgccc
     1561 ggcggcggct ggcttcgatg ccgagcaggg cggggaaggt cagcttgcca tgatcgtggt
     1621 cctttttcgt tcgcttgccc agggcggcgg cggtcccttc gcgatcgagc aggtcatcga
     1681 cgatctggaa cgccaggccg agccgctcgc cgtagcgatc gaggccggcg agctgctgct
     1741 cgctggcctg gccgacgatc ccgcccaatt gcaggctgac gcggatcatc gcgccggttt
     1801 tgcggcggtg gatcgcctcc agttgttcga gtcccaggtt ggcaaattct gctttcaggt
     1861 cgtcctcctg cccgccgacg agttggcagg cgccggcggc tttggcgagc tccgccacgc
     1921 agcgagcggc gactgccggg ggctcagtgc cggtggcgag gacctcgaag gcctgggcca
     1981 gcagggcgtc gccggcgagg atcgcggtgg cctcgccaaa cttggtgtgg cagctcggcc
     2041 ggccgcggcg catgtcgtcg tcgtccatcg ccgggaggtc gtcgt
//
LOCUS       DASUGB010000869         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1346565, whole genome shotgun sequence.
ACCESSION   DASUGB010000869 DASUGB010000000
VERSION     DASUGB010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346565"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="VFV87_16485"
     CDS             <1..1953
                     /locus_tag="VFV87_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105419.1"
                     /translation="GKVAIGWDTYDKGDYDVFVREFDTAGKADEPRPVANSADYEARP
                     ALTYDQSGSLWVAWEQSGPTWGKDWGALVQREGIGLYRDRQIGLAVWKDGQWMEPPEL
                     VAQSLPGAQPRRRVNNQRVPAIEPGAESRAAGQEAEARRDNVHNNIARLICDTQGRIW
                     LLARSRQNDFRSQIGSLWLTHAAYYDGDHWTGPILVPHTDNLLYNSPAATPLPDGGLL
                     IAHSSDHRQDRHIARRGANALGILEGVDPFENDMYLSRLEISGKAAAPALVLSKVVPD
                     PSAQPTAATLKERDELARIRGYRANIAGQELQIVRGEFHRHTEISGDGGNDGPLEDMW
                     RYAIDVAAMDWLGCGDHDNGGHREYPWWLTQKTTDAFHLPGRFEPPFTHERSVRYPEG
                     HRNVVIIQRGVRTLPRLPITDRTKEIHAPDTQMLYKYLKLFDGVCASHTSATSMGTDW
                     RDNDPLVETMVEIYQGARQNYERPGAPRSPSEGDAIGGWEPKGFVNLALLKGYRFSFQ
                     SSSDHGSTHISYALVYAENNSREAILKAMKQRHTYAATDNIIADFRCTSGGRDYMLGD
                     EFSAKEPPTLKIKLIGTMPFANVTLIKDDVEIPLEAGSKSEIDLTWTDPSPIAGKTSY
                     YYVRGDQEDGELVWASPMWITYEPGK"
     gene            1993..3093
                     /locus_tag="VFV87_16490"
     CDS             1993..3093
                     /locus_tag="VFV87_16490"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5105420.1"
                     /translation="MSSCWLACALTILGHNPDTSYARIEISADQLHTRLTYDIFTLLT
                     VVQLDDNADRQVSRAEMERHAPAIGQFLRDHIGLAISADDDSADLGEPAGFVWPPDTG
                     DAIRQADYHAATGLIHFDFVRPLSEVPAEVAVYFGFFETFNDRHTVLGVFEIVGDEYE
                     VTFTAYEPDFTYDTSVRPTMLARLWRFFKLGVEHIFLGYDHICFLIALIIVSKFREVV
                     KVVTSFTVAHSITLILATLQVVELPTWLVETCIAATIVYVAVENIFVKEPKHRWWLTF
                     FFGLVHGFGFANVLREMGLPTHGLIRCLLAFNVGVEVGQLAIATALLPLAMVLARWRH
                     GRKVVVAISLLLAIFGTAWFIDRALGLEAMPF"
     gene            complement(3111..>3561)
                     /locus_tag="VFV87_16495"
     CDS             complement(3111..>3561)
                     /locus_tag="VFV87_16495"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105421.1"
                     /translation="LLLLTAMICIVSGISILVSIYNSMSERRHEIAVIRALGAGRGTV
                     MTIILLEATLLALGGGIVGWLLGHAGVWACSPLIEEQTGVQVGFYNWEPAFRFLNWLG
                     VSGAAAEQYTLPVESVLIPALILLAILVGLWPAISAYRTDVARSLGK"
BASE COUNT          671 a         1173 c         1125 g          592 t
ORIGIN      
        1 ggcaaggtgg ccatcggctg ggatacctat gacaagggag actacgacgt ctttgttcgc
       61 gagttcgata cggccggcaa ggcggacgag ccccgtcccg ttgccaattc ggcggactac
      121 gaggcccgcc cggctctcac atacgatcaa agtggctcgc tgtgggtcgc ctgggagcag
      181 agcggcccga cgtggggcaa ggactggggc gctctcgtcc agcgcgaggg gatcggcctg
      241 tatcgcgacc gccagatcgg cctggccgtc tggaaggacg gccagtggat ggaaccgccg
      301 gagctggtcg cccagtcgct tccgggtgct cagccgcggc ggcgcgtgaa caaccagcgt
      361 gttccggcga tcgagcccgg cgcggaatcg cgggccgcgg gccaggaagc cgaagcccgc
      421 cgcgataatg ttcacaacaa catcgcccgc ttgatttgcg acacgcaggg gcgaatctgg
      481 ctgctggccc gctcgcggca gaacgatttc cggtcgcaga tcggctcgct gtggctgacg
      541 cacgccgcgt attacgacgg cgaccactgg accgggccga tcctcgtgcc gcacaccgac
      601 aacctgcttt acaacagccc ggccgccacg ccgctgccgg atggcgggct gctgattgcc
      661 cattccagcg atcaccgcca ggatcggcac atcgcccgcc gcggcgccaa cgccctgggc
      721 attctcgaag gcgtggatcc cttcgagaac gacatgtacc tcagccgact ggagatcagc
      781 ggcaaggcgg ctgcacccgc gctagtgttg tcgaaggtcg tacccgatcc ctcggcccag
      841 ccaaccgctg ccacgctcaa agagcgggac gagctggcgc ggattcgcgg ctaccgggcg
      901 aacatcgcgg gacaggagct gcagatcgtc cgcggcgaat tccaccgcca taccgaaatc
      961 agcggcgacg gcggcaatga cggcccgcta gaggacatgt ggcggtacgc catcgacgtg
     1021 gccgcgatgg actggctcgg gtgcggcgac cacgacaacg gcggccaccg cgaatatccc
     1081 tggtggctga cgcaaaagac aaccgacgcg ttccatctgc ccggccgctt cgagccgccg
     1141 ttcacgcacg agcggagcgt ccgctatccc gaggggcacc gcaatgtcgt catcatccag
     1201 cgcggcgtcc gcacgctgcc gcggctgcca atcactgacc gtacgaagga gattcacgct
     1261 cccgatacgc agatgctcta caagtatttg aagctgttcg acggcgtctg tgcctcgcac
     1321 accagcgcca cgagcatggg caccgattgg cgcgacaacg atccactggt cgaaacgatg
     1381 gtcgagattt atcaaggggc gcgccagaac tacgagcggc ccggcgctcc ccgcagcccg
     1441 agcgaagggg acgccatcgg cggctgggag cccaagggct tcgtcaacct ggctttgctg
     1501 aagggctata ggttctcgtt ccagtcgagc agcgaccacg gctcgacgca catcagctac
     1561 gccctggttt atgccgagaa caactcgcgc gaggcgattc tcaaggcgat gaagcagcgg
     1621 cacacctacg ccgcgaccga caacatcatc gccgatttcc gctgcaccag cggcgggcgc
     1681 gattacatgc tgggcgatga gttctcggcc aaggaaccgc ccacgctcaa gatcaagctc
     1741 atcggcacga tgccgttcgc caacgtcacg ctgatcaagg acgacgtcga aatcccgctc
     1801 gaggccggca gcaagagcga aatcgacctg acctggaccg atcccagccc gatcgccggc
     1861 aagacgagct attactacgt ccgcggcgat caggaagacg gcgagctggt ctgggcctcg
     1921 ccgatgtgga ttacgtacga accggggaag tagggggtca ggagtcagta gtcaggagtc
     1981 aggagtcagt acatgtcgtc gtgctggctg gcctgcgccc tgacaatcct cggccacaat
     2041 cctgatacaa gctatgcccg cattgaaatc agcgcggacc agctccacac gcggctcacg
     2101 tacgacatct tcacgctgct gacggtcgtg cagctcgacg acaatgccga ccggcaggtc
     2161 tcgcgggcgg aaatggagcg acacgcgccg gccattgggc aattcctgcg cgaccatatc
     2221 ggcctggcga tcagtgcgga cgacgattcg gccgatttgg gcgagccggc cgggtttgtc
     2281 tggcctccgg acacgggcga cgccatccgg caagccgatt accacgccgc caccggcctg
     2341 atccacttcg atttcgtccg gcctctgtcg gaagtgccgg cggaagtggc cgtctatttc
     2401 ggcttcttcg agacgttcaa cgaccggcac accgtgctgg gcgtctttga aatcgtaggg
     2461 gacgagtacg aagtcacctt caccgcctac gagcccgatt tcacgtacga caccagcgtg
     2521 cggccgacaa tgctggcccg gctgtggcgg ttcttcaagc tcggcgtgga gcacatcttt
     2581 ctgggctacg accacatctg ctttttgatt gccctgatca tcgtgagcaa gttccgcgag
     2641 gtcgtgaagg tcgtcacgtc attcaccgtg gcgcacagca tcacgctgat cctggcgacg
     2701 ttgcaggtcg ttgagctgcc gacgtggctg gtcgaaacgt gcatcgccgc gacgatcgtc
     2761 tatgtcgccg tcgagaatat cttcgtcaag gaaccaaaac accgctggtg gctgacgttc
     2821 ttcttcggcc tggttcacgg ctttgggttt gccaacgtcc tgcgcgaaat gggcctgccg
     2881 acgcacggcc tgatccgctg cctcctggcg ttcaatgtcg gcgtcgaggt cggtcagctc
     2941 gccatcgcca ccgcgctgct gcccttggcg atggtccttg cccggtggag gcacggccgc
     3001 aaggtcgtgg tggccatctc actgctcctg gcgatcttcg gcacggcctg gtttatcgac
     3061 cgggcgctgg ggctggaagc catgcccttt taaccgccgg atgacggtgt tcacttcccc
     3121 agcgagcgag cgacgtcggt gcggtaggcg ctgatcgcag gccacagacc gacgaggatc
     3181 gccagcagga tcaaggcggg aatcagaacc gactcgaccg gcagcgtgta ctgctcggcc
     3241 gcagcgcccg agacccccag ccagttcagg aaccggaacg caggctccca gttgtaaaag
     3301 ccgacctgca cgccggtctg ctcctcgatc agcgggctgc aggcccacac gccggcgtgc
     3361 cccagcagcc agccgacaat accgcccccc agggccagca gcgtcgcctc cagcagaatg
     3421 atcgtcatca cggtgccgcg gccggcgccg agggcccgga tcacggcaat ctcgtgccgc
     3481 cgctcgctca tcgagttgta aatgctcacc aggatgctga tccccgagac gatgcagatc
     3541 atcgcggtga gcaagagcag c
//
LOCUS       DASUGB010000870         8168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1208168, whole genome shotgun sequence.
ACCESSION   DASUGB010000870 DASUGB010000000
VERSION     DASUGB010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208168"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFV87_16500"
     CDS             <1..1174
                     /locus_tag="VFV87_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105422.1"
                     /translation="LLNQAEQQAAEQLAQQQAAGGEFDNRGRLNNYWMQQDVKRSKNV
                     VSGLGSNFDGSNEVTATPPAKGKELAFNKEWLEQNRLQTEGEKAGADRPARPGTADQA
                     GGGQMGSRSRYFRGEGKPSFGDDGKRGDGAQQQSGQKAPEIANQQQLGVLQQQLQQQT
                     EQFKSALTESGEQAQKLSRYQQRLDDEIRDQQRQPKADLDLPFRQGQFNNDTPTFGGF
                     DIPGGGAHPGQSMGGGGIAPSAGPSGITADPNAPPVAVPMPPAPVVDYAEVAAGLASL
                     DVALPERGRVYSFIVPRGDLEITARSISFATITRLIGLAGVAIAIVIVWALGREKSRR
                     IWIAMFGSFAFGIGLILLGLVSILFGLFPFAGLLALVIGIALAIRARLNRPAAAGA"
     gene            1307..3280
                     /locus_tag="VFV87_16505"
     CDS             1307..3280
                     /locus_tag="VFV87_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5105423.1"
                     /translation="MRLLAPPLLAFFAAIVSGPLSAEEPAKSKPSPQHTNRLAKETSP
                     YLLQHAHNPVDWYPWGEEALAKAKKENKLIFLSVGYSSCHWCHVMERESFLDKEIAQF
                     LNEHYVCVKVDREERPDIDGIYMTALHVYQQLAGGRRGGGWPMSMFLTPEAEPVFGGT
                     YFPARDGDRYEGSTGFLTIVKTIQDLWAKSPERIREDAKTIAKFTQAELEQRRGALTV
                     PLDAALVAGVQEALAEQYDERYGGFGFDLGNPNRPKFPEPSNLVFLLVRVRRDKNEAA
                     QAMLLGTLEKMSLGGIRDHLGGGFHRYSVDRYWSIPHFEKMLYDNGQLASVYAEAYAL
                     TGREDFRRVASELCDFVLREMTDESGGFDAALDAESEHEEGKFYRWEKAEIEKALTRD
                     EFALFAFCYGLNGDPNFDEQYYAPQLPRTLAELAAQKNLTEEELEAKLVPIRKKLFDL
                     RARRPRPLTDTKILTADNGLMIGGLADAGRILKEPRYIEAAAEAAGFVLTTLRQPDGR
                     LYRTYAGGQARLNGYLNDYAFLADGLIRLYQATGDKRWLNEADAITQKQIELFADDTS
                     GAFFFTSKDHEELLARTKELADNANPAGNSVAATNLIFLAVELKKPDYLSRASKTIAP
                     TAAILQNSPGAAPWMATAIPALIEARASLTTQP"
     gene            3313..4476
                     /locus_tag="VFV87_16510"
     CDS             3313..4476
                     /locus_tag="VFV87_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5105424.1"
                     /translation="MPASSQTFHLSAQHDGLSLGQTLKRLLPERSWSQVRKLIAAQRV
                     QVNGNLCLDEGRRVKAGDVLKIAERPLARPADESDIKIVHLDEHLIVIQKPAGITTLR
                     HRAETDLPERRKQLQPTLDELLPRVLAGHLGIRLPDDTNPKRQRGSPNDTNPKRQRGS
                     ARARRGTATTGRGFDKRLQVRAVHRLDRDTSGLMVFARTPEAEQKLIRMFARHAVQRA
                     YVAVVHGQLTEPRTIATLLARDRGDGLRGSVGSNVGSDRRAEPEVPTDAAREVPTGGR
                     DLQRAITHVRPMEQLVGYTIVECRLETGRTHQIRIHLAEIGHRLCGEKTYTHAPGQQP
                     QPDTSGAPRQALHAAEIAFTHPITNQPLRFRMPLPQELKDWLLSLRQALRQRG"
     gene            4505..5116
                     /locus_tag="VFV87_16515"
     CDS             4505..5116
                     /locus_tag="VFV87_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5105425.1"
                     /translation="MSTVPKRLLTEQEYLAKERQAPFRSEFSRGEMFAMAGATREHNL
                     ITGNVARNLANQLDGRPCETYSTDMRVRITPTGLYTYPDVVVACGSPQFADNEQDVLL
                     NPTVLIEVLSPSTASYDRGDKRVHYLQLDSLRELLLVEQEFPAVEHHIRQPNGDWIER
                     QIEGPEARIELPSIGCVLTFAEIYARITFPPDARRPLRPMVDE"
     gene            5135..5860
                     /locus_tag="VFV87_16520"
     CDS             5135..5860
                     /locus_tag="VFV87_16520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5105426.1"
                     /translation="MPLSESEIVARLKDNVARVRERMAAAAARAGREPHEILLVGVTK
                     YVNTDLMRLLVEETGVNHLGESRPQDLWRKVESLGGMQIAWHLIGHLQRNKVRRTLAA
                     CWGVVIHSVDSLRLLEEINREASEIGRPADVLLEVNISGDANKHGFAPDEMTATLASI
                     AAMQNVCVLGLMTMASLEGGADQARRDFAALRNLLDCLRPHCPEQIVLDQLSMGMSGD
                     YEIAIEEGATMVRVGSALFEGIV"
     gene            6046..6930
                     /locus_tag="VFV87_16525"
     CDS             6046..6930
                     /locus_tag="VFV87_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5105427.1"
                     /translation="MRFASLLALLLVLTAATAAPARDIYVDNLNGDDRRHGETPVSQG
                     AAGGPCRSIAKALRVAKPGDRIIVANTGQPYRECVSIQGGRHSGSGDYPFEIIGNGAT
                     LDGRISLAENEWEFVAADTFRTRPPRMSYQQLFLGDVVAARRFEEGGRFPALEPLEWI
                     LLDGWIYFRVEPGKLPQSYDLSCCGHQVALTLYDVHDVIVRDLVVRGYQLDGVNCHDN
                     VRRSDLINLTCIENGRSGISIGGASRVRIDTCTAAGNGAAQLRVEGYCIVQTIENTFD
                     GTSAPAIVREGGTIIEEK"
     gene            6934..7896
                     /locus_tag="VFV87_16530"
     CDS             6934..7896
                     /locus_tag="VFV87_16530"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5105428.1"
                     /translation="MFGIRYLKVAPTTYVLHYKGGQVRREGAGLSFFYFAPTSELVLV
                     PIESTDVPFVFNEVTSDFQDATIQGELTYRIADPKRVAAVLNFSVDERQHHRSEDPQK
                     LGERLVHAARIGARAFTQKRPLKEVLVSSEPLMNETLVSLRNSPAVQMLGIEVLGLSI
                     VSITPTPEMTKALQADAREELLKKADEAIYARRNAAVAMERTIKENELQTEITVEEKR
                     RQVRETQMAAEIAVEEQRAKLVDERVHNERKEADARAHALKVTLDPLKGVDWKILMAS
                     GGGGLDPKLNIALAFRELAENAQKIGELNVSPDLLNTLLKSGKA"
     gene            7916..>8168
                     /locus_tag="VFV87_16535"
     CDS             7916..>8168
                     /locus_tag="VFV87_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105429.1"
                     /translation="MALPGIAIVTSPTRLEGLRRRWGTLGQAKFLLKQAHVHERARRA
                     GNAEAQQGARQRRAAKAATVVLPEAQQQADFDEYLEENVT"
BASE COUNT         1602 a         2706 c         2563 g         1297 t
ORIGIN      
        1 cctcttgaac caggccgagc agcaagccgc cgagcagctt gcccagcagc aggcggcggg
       61 gggcgagttc gacaatcgcg gccggctgaa caactactgg atgcagcagg acgtgaagcg
      121 gtcgaagaac gtcgtttccg gcctggggag caatttcgac gggagcaacg aagtgacggc
      181 gactccgccc gccaagggga aggagctggc gttcaacaag gagtggctcg agcagaaccg
      241 gctgcaaacg gagggcgaga aggcaggcgc cgacaggcct gcccggccgg gaacggccga
      301 tcaagcaggc ggcggacaaa tgggcagccg ctcgcgctac ttccgtggcg aaggcaagcc
      361 gtccttcggc gacgacggca agcgcggcga cggcgctcag cagcagagcg gccagaaagc
      421 ccccgaaatc gccaaccaac agcagctcgg cgtgctccag cagcagcttc agcagcaaac
      481 cgagcagttc aagagcgccc tgaccgaatc gggcgagcag gcgcaaaagc tcagccgcta
      541 tcagcagcgc ttggacgacg aaatccgcga tcaacagcgc cagccaaagg ctgatctcga
      601 cttgccgttc cgccaagggc agttcaacaa cgacacaccg acgtttggag gattcgacat
      661 tcccggcggc ggagctcatc ccggccagag catgggcgga gggggaattg ctcccagcgc
      721 cggcccttcc ggaatcaccg ccgaccccaa cgcacctccc gtcgccgtcc cgatgcctcc
      781 ggcgccggtc gtcgattatg ccgaagtcgc ggccggcctg gccagcctcg acgtggccct
      841 ccccgagcgt ggccgcgtct attcgttcat cgtgccccgc ggcgacctgg aaatcactgc
      901 ccgctcgatc tccttcgcca ccatcacccg gctgatcggc ctggcgggcg tggcgatcgc
      961 gatcgtcatt gtctgggccc tcggccgcga gaagtcgcgc cgcatctgga tcgcgatgtt
     1021 cggctcgttc gccttcggca tcgggctgat cctgctgggc ctggtcagca tcttgttcgg
     1081 cttgttcccg ttcgcgggat tgctcgccct ggtcatcggc atcgccctcg ccatccgcgc
     1141 tcgcctgaac cgccccgctg ccgcaggcgc ataagacagg aagatcaacg acaggaaaat
     1201 gtggcgaatt gtgccgtccc cattttcctg tcggcaatct tcctgtcatt gcggagtggt
     1261 acaatcgagt cgaacccgcc gctgccttgc ccgccaggaa ttgcacatga gacttctcgc
     1321 gccaccgcta ctcgcattct tcgcggcgat cgtctccggg ccactgtccg ccgaggaacc
     1381 ggctaagagc aagccatcac ctcagcacac caaccgcctg gccaaggaaa cgagcccgta
     1441 cctgctgcag catgcgcata atccagtcga ttggtatccc tggggcgagg aggccctcgc
     1501 gaaagcgaag aaggaaaaca agctgatttt tctcagcgtc ggctattcga gctgccactg
     1561 gtgccacgtg atggagcgcg agagcttttt ggacaaagag atcgcgcaat tcctgaacga
     1621 gcactacgtc tgcgtcaagg tcgatcgcga ggaacggccc gatatcgacg gcatctacat
     1681 gaccgccctg cacgtctatc agcagcttgc gggcggccgc cgcggtggcg gctggccgat
     1741 gtcgatgttc ctcacccccg aagccgagcc ggttttcggc ggcacctatt tcccggcccg
     1801 cgacggcgac cgctacgagg gctccacggg ctttctgaca atcgtcaaga cgatccagga
     1861 cctgtgggcc aaatcgcccg agcggattcg cgaagacgcc aagaccattg ccaaattcac
     1921 ccaggcagag ctggaacagc gccgcggcgc cctgaccgtg ccgctcgacg cggccctggt
     1981 cgccggcgtc caggaagccc tggccgagca atacgacgag cgctacggcg gcttcggctt
     2041 cgacctgggg aatcccaatc gcccgaaatt cccagagccg agtaacctcg tttttctgct
     2101 ggttcgcgtg cggcgcgaca agaacgaggc agcccaggcg atgcttcttg gcacgctgga
     2161 aaaaatgtcg ctcggcggca tccgcgatca tctgggcggc ggctttcacc gctatagcgt
     2221 cgatcgctac tggagcatcc cgcatttcga gaaaatgctc tacgacaacg ggcaactggc
     2281 gagcgtctat gccgaagcat atgcactcac cggccgcgag gacttccgcc gcgtcgcgag
     2341 cgagctctgc gacttcgtcc tccgcgagat gactgacgaa tctggcggct tcgacgcggc
     2401 actcgatgcc gaatcggaac acgaggaagg gaagttctat cgctgggaga aagccgagat
     2461 cgagaaagcg ctcacgcgcg acgagttcgc gctgttcgcg ttctgctatg gcctcaacgg
     2521 cgatcccaat ttcgacgagc agtactacgc cccgcagctc ccgcggactc tggccgagtt
     2581 ggctgcgcag aagaacctga ccgaagagga gctggaagcg aagctcgtcc cgatccgcaa
     2641 gaagttgttc gacctgcggg cccggcgccc gcgaccattg accgacacga aaatcctcac
     2701 cgccgacaat ggcctgatga tcggcggcct cgccgacgcc ggccggattc tcaaagagcc
     2761 ccgctacatc gaggccgccg ccgaagccgc aggattcgtc ctcaccacgc tccgccagcc
     2821 ggacggccgg ctttaccgca catatgccgg cgggcaggcc aggctcaacg gctatctgaa
     2881 cgactatgcc tttctcgccg acggcctgat ccggctctac caggcgaccg gcgacaagcg
     2941 ctggctcaat gaagccgacg cgataacgca gaagcaaatc gagctattcg ccgacgacac
     3001 aagcggggcg ttcttcttca catcgaagga ccacgaggag ctgctcgccc gcaccaaaga
     3061 actggccgac aacgccaacc cggccgggaa ctccgttgcc gcaaccaacc tgatcttcct
     3121 cgccgtcgag ctcaagaagc ccgattacct gtcgcgggcc agcaagacca tcgcccccac
     3181 tgccgccatc ctgcagaact cccctggcgc ggctccctgg atggcgacgg caattcccgc
     3241 actaatcgaa gcgcgagcgt cgctgacgac gcaaccgtga gggaaggaac agacaactct
     3301 gcgctgccgc cgatgcccgc ctcctcgcag acctttcact tgtcggcaca gcacgacggc
     3361 ctctcgcttg gccagacgct gaagcgattg ctgccggagc ggagctggtc gcaggtgcgg
     3421 aagctgattg cagcgcagcg ggtgcaggtg aatgggaatc tgtgtctgga tgaggggcgg
     3481 cgggtgaagg caggcgacgt gctgaagatc gccgagcgac cgctcgccag gcctgcggat
     3541 gaaagcgaca tcaagattgt ccatctcgac gagcatttga tcgtcatcca gaagccggcg
     3601 ggcataacga cattgcggca tcgggcggag accgacctgc ccgagcgccg caagcagctc
     3661 cagccgacgc tcgacgagct gttgccgcgg gtgctggccg gccacttggg aatccgcctg
     3721 ccggacgaca ccaacccgaa gcgtcagcga gggagcccca acgataccaa cccgaagcgt
     3781 cagcgaggga gtgctcgcgc gcgacgggga accgcgacca ctggtcgcgg ctttgacaag
     3841 cgattgcagg tccgggcggt tcaccgcctc gaccgcgata cgagcggact gatggtcttt
     3901 gcccgcacgc ccgaggccga gcagaagctg atccgcatgt tcgccaggca cgccgtgcag
     3961 cgggcgtacg tcgctgtcgt ccacggccag ctcaccgagc cgcgaacgat cgccacgctg
     4021 ctcgcccgcg accgcggcga cggcttgcgg gggagcgtcg gctcgaatgt aggttccgac
     4081 cgccgggcgg aaccagaagt gccaaccgac gccgcccgcg aggtcccgac cggcggtcgg
     4141 gacctacagc gggccatcac ccacgtccgc ccgatggagc agctcgtggg ctacacgatc
     4201 gtcgaatgcc gcctggaaac cggccgcacg caccagatcc gcatccacct ggccgaaatc
     4261 ggccaccgcc tctgcggcga gaagacctac acgcacgccc ccggtcaaca gccgcagccc
     4321 gacaccagcg gcgcaccccg ccaggccctg cacgccgccg aaatcgcctt cacccacccc
     4381 attaccaatc agcccctccg cttccgcatg ccgctgccgc aggaactcaa ggactggctg
     4441 ctctcattgc ggcaagcgct gcgccagcgc ggatagaata caatagcatt cagtgaggtc
     4501 ttcaatgtcc accgttccca agcgattgct gaccgagcag gagtacctgg ccaaggaacg
     4561 ccaggcgcca tttcgcagcg aattctcccg cggtgagatg ttcgccatgg cgggggccac
     4621 gcgcgaacac aacctgatta ccggcaacgt cgctcgcaac ctggccaatc agcttgacgg
     4681 tcgcccgtgc gagacctatt cgacggacat gcgcgtgcgc atcacgccga ccggcctgta
     4741 cacctatccc gacgtggtcg tcgcttgcgg cagtccccag tttgccgaca acgagcagga
     4801 tgtgctgctc aatccgacgg tcctgatcga ggtgctgtcg ccttccacgg ccagctacga
     4861 ccgcggcgac aagcgcgtgc attacctcca gctcgattcg ctccgagagt tgctcctggt
     4921 cgagcaggaa ttccccgcgg tggagcacca tattcgccag cccaacggcg actggatcga
     4981 gaggcagatt gagggtccgg aagcgcgcat cgaactcccg tccatcggct gcgtgctgac
     5041 gtttgccgaa atctatgccc ggattacatt tccaccggac gccagacgcc cgctgcgacc
     5101 gatggtggat gagtaacgcc gccgcgctgg caccatgccg ctctccgagt ctgaaatcgt
     5161 cgccaggttg aaagacaacg tggcccgcgt ccgggaacga atggccgccg cagcggcacg
     5221 ggccggccgc gagccgcacg agatcctcct cgtcggtgtt accaagtacg tcaacaccga
     5281 cctgatgcgg cttctggttg aggagaccgg cgtcaatcac ctaggtgaga gtcgtccgca
     5341 ggacttatgg cggaaggtcg aatcgctcgg cgggatgcaa atcgcgtggc atttgatcgg
     5401 tcatcttcag cgaaacaagg tccggcgaac gctcgctgcg tgctgggggg tcgtcatcca
     5461 ctccgtcgac agcctgcgac ttctggagga gatcaatcgg gaagcctccg agatcggtcg
     5521 gccggcagat gtgctgctgg aagtgaatat ctctggcgac gcgaacaagc acggtttcgc
     5581 gccggacgag atgacggcga ctttggcttc gattgccgca atgcaaaacg tgtgcgtgct
     5641 gggcctgatg acgatggcct cgctggaggg aggcgcagat caagcgcggc gtgactttgc
     5701 tgccctgcga aacctgctcg actgcctccg gccgcattgc cccgagcaaa tcgtgctgga
     5761 tcaactgtcg atgggcatgt cgggcgacta tgagatcgcg atcgaggagg gtgcgacgat
     5821 ggtccgggtg gggtcggcgc tgtttgaggg gatcgtttag agaacactgt gtagcggttg
     5881 tgcgggtcgc gccggcggaa gggctggcgc atggggcgtg ctacacttgc gaagcttcca
     5941 cgtccaaggg gtcgggaatc aattgcggcc acaatcttgc catctggaaa gcagtctgcc
     6001 gcaattggct cccgacccct tccgcgccgc catccccata cgcccatgcg ctttgcatcc
     6061 ctgcttgccc tgctgctcgt cctgaccgcc gcaacagccg caccggcacg ggacatctac
     6121 gtcgataact tgaacggcga cgaccgccgg catggcgaga cgccggtctc acagggtgcg
     6181 gcgggcggac cttgccggag catcgccaag gccctccgcg tcgccaagcc gggcgaccgg
     6241 atcatcgtcg ccaacacggg ccagccgtac cgcgagtgcg tctcgatcca agggggccgg
     6301 cacagcggca gcggcgatta tccgttcgag atcatcggta acggggcgac gctcgacggc
     6361 cgcatctcgc tagccgaaaa cgagtgggaa ttcgtcgccg cggacacgtt ccgcacccgc
     6421 ccgccgcgga tgagttatca gcagctcttt cttggcgacg tggttgccgc ccgccgcttc
     6481 gaggaaggcg gcaggttccc ggccttggag ccgctcgagt ggatcctgct ggacggctgg
     6541 atctatttcc gcgtcgagcc cggcaagctg ccacagtcgt acgacctgtc gtgctgcggg
     6601 catcaggtgg ccctcacgct ctatgacgtc cacgacgtga tcgtccgcga cctggtcgtc
     6661 cgcggctacc agctcgacgg cgtgaactgc cacgacaatg tccgccggtc ggacctgatc
     6721 aacctgacct gcatcgaaaa cggccgcagc gggatcagca tcggcggcgc cagccgcgtg
     6781 cgcatcgata cctgcaccgc cgcaggcaac ggcgcggccc aactccgcgt cgagggctat
     6841 tgcatcgtgc agacgatcga gaatacgttc gacgggacca gtgcccccgc gatcgtccgc
     6901 gaggggggca cgatcatcga ggagaaataa gccatgttcg gcatccgcta cctcaaagtc
     6961 gccccgacga cctacgtcct gcactacaag ggcggtcaag tccgccgcga aggggcgggc
     7021 ctgtcgtttt tctacttcgc tcccacgtcg gagctggtcc tggtgccgat cgagagcacc
     7081 gatgtcccct tcgtgttcaa cgaggtgaca tccgactttc aggacgcgac gatccagggt
     7141 gaattgacgt atcgcattgc cgatcccaag cgtgtggccg cggtcctgaa cttcagcgtc
     7201 gatgagcggc agcatcaccg cagcgaagac ccgcagaagc tgggcgagcg cctggtgcac
     7261 gccgcgcgga tcggggcgcg ggccttcacg cagaagcgac cgctcaagga ggtgctcgtt
     7321 tcgtccgagc cgctgatgaa cgagacgctt gtcagcctgc ggaattcgcc cgcggtgcag
     7381 atgctcggca tcgaagtgct gggtctgtcc atcgtctcga tcacgccgac gcccgaaatg
     7441 accaaggccc tgcaagccga cgcccgcgag gaactgctga agaaagctga cgaagcgatc
     7501 tatgcccgcc gcaacgcggc ggtcgcgatg gagcggacga tcaaggaaaa cgagctgcag
     7561 accgagatca ccgtcgaaga gaagcggcgg caggtccgcg agacgcaaat ggccgccgag
     7621 atcgcggtcg aagagcagcg ggccaagctc gtcgatgaac gcgtgcacaa tgagcgcaag
     7681 gaagccgacg cacgggccca cgccctgaag gtcacgctcg atccgctcaa gggagtcgat
     7741 tggaaaatcc tgatggcttc cggcggcggc ggactggatc ccaagctgaa catcgcgttg
     7801 gccttccgcg agctggccga gaacgcccaa aaaatcggcg agctgaatgt ttcgccggac
     7861 ctgctcaata cgctgctcaa atctggcaaa gcatagtgga agcgtggcca tgggaatggc
     7921 gctgccggga attgccatcg tcacctcgcc gacgcgcctg gaagggctgc gccgccggtg
     7981 gggcacgctc ggccaggcca aattcctgct gaagcaagcc cacgttcacg agcgggcgcg
     8041 gcgcgccggc aatgccgagg cgcagcaggg cgcacgccaa cgccgagcag cgaaagcggc
     8101 gaccgtcgtc ctgcctgagg cccagcagca ggccgacttc gacgaatacc tggaagagaa
     8161 tgtcacgt
//
LOCUS       DASUGB010000871         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2041990, whole genome shotgun sequence.
ACCESSION   DASUGB010000871 DASUGB010000000
VERSION     DASUGB010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041990"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFV87_16540"
     CDS             <1..511
                     /locus_tag="VFV87_16540"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium uptake protein TrkA"
                     /protein_id="HEX5105430.1"
                     /translation="VLVGSIPLAVPGLSNPVRLGLAGGPLIVALILGRIGHLGGMVWH
                     MPLNANHAFREFGISLFFAALGLAAGDQFFAAVFSPRGLTWLVAGLIVSLVPLLTIGI
                     WSRAVEKMNFVTLTGMLAGGTTNPPALSFATNLCRSAAPSLAYATVYPLTTLLRILVA
                     QLLVLFLCG"
     gene            complement(465..2168)
                     /locus_tag="VFV87_16545"
     CDS             complement(465..2168)
                     /locus_tag="VFV87_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5105431.1"
                     /translation="MWLSADRSIALIVLALAGGAAGCRSLQPCALHTELPAGPISWES
                     SLQAACGELARGHDLEAAGDERCVDAYFRAAHLSWQAIAEAGACGCYGEATGCLLLAA
                     QRFGRLDPECGLVIHEGGRSDFVPVVHQGFAWGASDFQRLHPPPRGHEPLLSRRYGCQ
                     GWGVPLVVERRRNGGDPLEARFFPEKAFFGATAVLRFDEMTTILEFHNPLAARCLETD
                     AGPLPLAADLTAPLAQTLEEAPRTYFAGFVEPGRAAATSRLSFLEPYQPGKVPLVLIH
                     GLFSDPLSWADLVNDLRATPGFTDRYQLWVFRYPTGQGFLQSAAALRVELAAAAQELD
                     PGCSDQALHQMVLIGHSMGGLIAKLQVTYSDELIWNRLANRPLEEIITTESTRARLAE
                     ICYFDPSPDVARVVFIASPHAGSLRSSELIGRGVSHLVEPSPQQAAMHEQLMRDNPDT
                     FNPQVERRFPTSIDMLAPQSPLLAAMQKMRLREGIKLHNIMGVSCPLSLDGPSDGVVS
                     ARSASHPCCQSVLAVGASHVKVHRSIQTGAEILRILDYQASAGPISRRETAPATAPRE
                     F"
     gene            complement(2327..>3077)
                     /locus_tag="VFV87_16550"
     CDS             complement(2327..>3077)
                     /locus_tag="VFV87_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5105432.1"
                     /translation="SQSAVNERGGAKTPLSLVFASAAICCVLLFLTGLFHTLPDAVLA
                     AVVLVAVAGLIDLPELRHLWRASRSDFAAAAVALAGVLLMGVLDGVLIAALISILMLL
                     VRNSRPPVAFLGRIPGTNRFSDLARHPDNEPIPGVLAFRVQASLLYFNVDHILETVRQ
                     RAREQTGLRRVVCDLSNVPYVDIAGARMLRRLYEELAAMGVDFKVVEAHGPVRDILRA
                     EGLEERLGAISRRTTLAQVLAEGSGPDTYAE"
BASE COUNT          559 a          987 c         1019 g          512 t
ORIGIN      
        1 tgtgctcgtc ggctcgattc ccctggccgt gccgggtttg tccaacccgg ttcgcctcgg
       61 gttggctggc gggccgttga tcgtggcgct gatcctggga cgcatcggcc acctcggcgg
      121 aatggtctgg cacatgcccc tcaatgcgaa ccacgccttt cgcgaattcg gcatctcgct
      181 cttctttgcc gccctcggcc tggcagccgg cgaccagttc ttcgccgcgg tcttcagtcc
      241 gcgcggcctg acgtggctcg tcgccggatt gatcgtttcg ctcgttccct tgctgaccat
      301 cgggatctgg tcccgcgccg tggagaagat gaatttcgtg acgctcaccg ggatgctcgc
      361 cggcggaacg acaaacccgc cggccttgag cttcgccacg aacctgtgcc gctccgctgc
      421 cccgtcgctc gcctatgcaa cggtctatcc gctaacgacc ctgctcagaa ttctcgtggc
      481 gcagttgctg gtgctgtttc tctgcggctg atgggaccgg cgctcgcctg gtagtcgagt
      541 atccgcagga tttccgcacc ggtctgaatc gaccggtgga ccttgacgtg cgacgctccg
      601 acggccagca cgctttggca gcagggatgc gaggcgctgc gagcggagac gacgccgtcc
      661 gaaggcccat ccagcgacag cgggcacgag acgcccatga tattgtgcag cttgatcccc
      721 tcccgcagcc gcattttctg catcgcggcc aagagcggcg actggggggc cagcatgtcg
      781 atgcttgtgg gaaagcggcg ctcgacctgg ggattgaacg tgtccgggtt atcgcgcatg
      841 agctgctcgt gcatggcggc ttgctggggc gagggctcga cgagatgcga gacgccacgt
      901 ccaatcagct cgctggaacg cagcgagccg gcgtgcggcg aggcaatgaa gacgacgcgg
      961 gcgacgtcgg gcgacggatc gaaatagcaa atctcggcca atcgcgcgcg ggtggactcc
     1021 gtggtgatga tttcttcgag cggacgattg gccagccggt tccagatcag ctcgtcggaa
     1081 taggtcacct ggagcttggc gatcagcccg cccatgctgt ggccgatcag caccatctgg
     1141 tgcaaggcct ggtcgctgca gccgggatcg agctcctgcg ctgcggcagc gagttcgaca
     1201 cggagtgccg cggccgactg caaaaaccct tggccggttg gatagcgaaa gacccacagt
     1261 tgatagcggt cggtgaaacc cggtgttgcc cgcaaatcgt tcaccaggtc cgcccagctc
     1321 agcggatcgg aaaacagtcc gtggatgagc acgaggggaa ctttgcctgg ctggtaaggc
     1381 tccagaaaac tcagccgcga cgtggcggcc gcccgcccag gttcgacgaa gccggcaaaa
     1441 taagtgcgcg gcgcttcctc aagcgtctgc gcgagtggcg ccgtgaggtc ggcggccagc
     1501 ggcagcgggc ctgcatcggt ctcaaggcat cgtgctgcga gtggattgtg gaattccaag
     1561 atggtcgtca tttcgtcaaa gcgcagtacg gctgtggcgc cgaagaaggc cttctcggga
     1621 aagaatcggg cttccaacgg atcgccgcca ttgcgccgcc gctcgacgac cagcggcact
     1681 ccccagccct ggcagccata tctccgcgag agcagcggtt catggcctct tggcggcgga
     1741 tggaggcgtt gaaagtcgct ggccccccag gcgaagccct gatgaacgac agggacaaag
     1801 tcacttcggc ctccttcgtg aattaccagc ccgcactccg ggtccagccg gccaaaacgc
     1861 tgagcggcaa ggagcaggca gccggtcgct tcgccgtagc agccgcaagc gccggcctcg
     1921 gctatcgcct gccaggacaa gtgcgcggcc cgaaagtagg catcgacgca ccgctcgtcg
     1981 ccggctgcct caagatcgtg cccgcgggcc aattcgccgc aagctgcctg gagcgaagat
     2041 tcccaggaga tcggaccggc aggaagttcc gtgtgcagag cgcacggctg tagcgaccga
     2101 cagcctgcgg cgccgccggc cagcgcgaga acgatgagcg cgatcgatcg atctgccgag
     2161 agccacatgg actagccagc caaggtgccg cgtcacaggc gcctccatgc cagccgtccc
     2221 cgtccactgg ttcgcctcgt ccatcatcgg ccggcgacta cgtcaccctg gagcaggaat
     2281 cctccatcga gttgaccgct ggggggattt tgattcagcg ccgttcttat tcggcataag
     2341 tgtcagggcc gctcccctca gcgagcactt gggccagggt ggttcgacgt gagatggcgc
     2401 cgagacgttc ctccagtccc tcggcgcgca ggatgtcgcg caccgggccg tgggcctcaa
     2461 cgaccttgaa atccaccccc attgccgcca gttcttcata gagccgacgc agcatccgag
     2521 cgcccgcgat atccacgtag ggcacgttgg acagatcgca gacgacgcgg cgcaggccgg
     2581 tttgctcacg tgcacgctgc cggaccgttt ccaggatgtg atcgacgttg aaatagagca
     2641 gcgaggcctg cacgcgaaag gcgagcacgc cggggatcgg ctcgttgtcg ggatggcggg
     2701 cgaggtccga gaaccgattg gtgccgggaa tgcgacccag aaaggccacg ggcggacggg
     2761 aattacggac gagcagcatc aagatggaaa tcagtgcggc gatcagcacg ccatcgagga
     2821 cgcccatgag cagcacaccg gccagggcca cggccgcggc ggcaaagtcc gaccggctgg
     2881 cgcgccacag atggcgcagt tcgggcaggt caatcagtcc cgccacggcg accaggacaa
     2941 cggcggcgag cacggcgtcg gggagcgtgt gaaagaggcc cgtcaggaat agcagcacgc
     3001 agcagatcgc cgccgaagcg aacaccaacg acagcggcgt cttggccccg ccccgctcat
     3061 tgacggcgga ttgcgac
//
LOCUS       DASUGB010000872         5614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_791484, whole genome shotgun sequence.
ACCESSION   DASUGB010000872 DASUGB010000000
VERSION     DASUGB010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791484"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFV87_16555"
     CDS             <1..765
                     /locus_tag="VFV87_16555"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5105433.1"
                     /translation="WTDGLPRAKGHDGKTLSRNTPSLLNAGYYSLYTWNGRAKSLEEQ
                     ALMPIQSPDEMNQDLGELEQELNAVPGYVEQFQRVFGTKATRDGIAKALACFQRTLVT
                     KPSPLDRYLAGDKEALSPAAQRGMELFLGDAGCLRCHQGPRLSDGKFYRLGISLQDEG
                     LAAISKKPEDRGKFRTPTLRNIAQTGPYMPDGSLKTLDDVVTFYYRGAPTGKVDGLTP
                     DIQPLLGQSFSEIPDLVAFLESLTGEPPQITRPELP"
     gene            complement(769..1521)
                     /locus_tag="VFV87_16560"
     CDS             complement(769..1521)
                     /locus_tag="VFV87_16560"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5105434.1"
                     /translation="MLSFWLALLLVLIVALAAGAIASVAGFGIGSLLTPLLGLQVEMK
                     VAVAVISIPHFIATALRYWILRKHFNWRVLWSFGLTSAAGGLAGAFLGAFLASPLLTA
                     VPGVLLVFAGVMGVTGYASKMRFSGWTAWVAGVLSGAFGGLVGNQGGIRSAALMGFDI
                     RKDAFVATATAVGLLVDGARMPVYFLTQWSDIAKAWPYVAAATVGVVAGTIAGERLLR
                     RIPETTFRKIVSAIILVLGIALLTGAAWTLWK"
     gene            complement(1534..2127)
                     /locus_tag="VFV87_16565"
     CDS             complement(1534..2127)
                     /locus_tag="VFV87_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105435.1"
                     /translation="MSRSTALFILTAFLTGGALGILIRPMSSDLGTVGSAAYGQQSAK
                     APPLDLAALAAEVERLKKIVPDQSHAMSDVDYHFSNLWFAGKAQNWPLADFYWKESLA
                     HMKWAVRIIPVRKDNAGKEVKLEDILKSIENSPYMQMGEVIKQQDAAKFEPTYKYIVE
                     GCYSCHKASDKPYLHPHIPERPASAMINFDPQADWPK"
     gene            2442..3080
                     /locus_tag="VFV87_16570"
     CDS             2442..3080
                     /locus_tag="VFV87_16570"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5105436.1"
                     /translation="MEAPSAEKSAEAISAPEDLMREHGVLNRVLLIYEEGVRRLRKGE
                     EAPPEIFQRGAQLVRRFVEDYHEQLEQNFIFPQFEKRQAHALLVRVLRQQHDAGRKLT
                     DAIQRASAPDQFVKAPAREQLARTCEAFCRMYRPHEAREDTVLFPALYKIVPATEIKD
                     LGEQFEQQEHKLFGEEGFQKTVEQVAEIEKLLGIYDLAVFTPKSPVTADGSR"
     gene            3067..3516
                     /locus_tag="VFV87_16575"
     CDS             3067..3516
                     /locus_tag="VFV87_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5105437.1"
                     /translation="MALVNKLLVTDTPAAAILVRVLVGAVFLSEGIQKFLLPAAVGAG
                     RFAKIGIPSPEFTAPFVGCFEIACGVLLILGLFTRLGAIPLIVIMLVAISTTKIPILM
                     NEGFWKAAHESRTDWSMLLGSIFLLIVGAGPWSLDAWLGANARARSR"
     gene            complement(3503..3883)
                     /locus_tag="VFV87_16580"
     CDS             complement(3503..3883)
                     /locus_tag="VFV87_16580"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5105438.1"
                     /translation="MASSVLKHLFTGFVRLHILYHADKQPVCGVELMEELQHHGYKIG
                     PGTLYPVLHGLEESGFVKAKDEVVGGKRRKNFRTTARGRKLLAEAREKLVELASEIVD
                     DKDAMAERKKGRKEEGKVIGFIEI"
     gene            4020..>5614
                     /locus_tag="VFV87_16585"
     CDS             4020..>5614
                     /locus_tag="VFV87_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5105439.1"
                     /translation="MNLIAAALRRPLTVMVAIAAIAFGFTLAVGKSVCDQAGIPYPAG
                     LPKGMEVDIFPALNLPVVYVCQPYGGMDPAQMEGYLTNFYEYHFLYISNIHHVESRNV
                     QGVALMKLHFHPGTNMAQAMAETISYVNRSRAFMPPGTVSPFVMRFDTGSVPVGYLVL
                     RSDTRTVAEINDLALFSVRPTFAGLPGVSAPPPFGAGARSLVVTVDPQRLQSYGMSPD
                     EVIVAVTKGNTLGPSGNVPIGDKFAIVPVNSVVKDVNQMLSIPIRTGPDAVYLRDIGT
                     VADAADAPTGYALINGRRAVYILATKRADASTLSVVNTIKRALPEMQAALPPDIKVSF
                     EMDQSPYVTRAVVGVLTEGTLGAVLVGLMVLIFLWDWRSALVVVLNIPLALMASVLAL
                     WITGYTINVMTLGGLALAIGVLVDEATVEVENIHTQMRHTGSIARAVRLGNAVTAVPR
                     LLAMLCILAVFVPAFFMEGAAQALFAPLALAVGFAMVASYLLSSTFVPVVSVWLLRHA
                     HGGQGRGNSSPLSPRGRGAGGEGA"
BASE COUNT         1024 a         1820 c         1698 g         1072 t
ORIGIN      
        1 tggaccgacg gattgccgcg ggcgaagggc cacgacggca agaccctcag ccgcaatacg
       61 ccctcactcc tcaatgcggg ctactattcg ctctacacct ggaacggccg cgccaagtcg
      121 ctcgaagagc aggcgctgat gccgattcag tcgcccgacg aaatgaatca ggacttgggc
      181 gagctcgaac aagaactgaa tgccgtgccg ggctatgtcg agcaattcca gagagtcttc
      241 ggcacgaagg cgacgcgcga tggcattgcc aaggcgctgg cctgcttcca gcggacgctg
      301 gtcaccaagc cgtcgccgct cgatcgctat cttgccggcg ataaagaggc cttgtcgcct
      361 gccgcccagc gcggcatgga gctgttcctg ggcgacgccg gctgcctgcg ttgccaccag
      421 ggaccgaggt tgagcgacgg caagttctat cgcctcggta tttcgctgca ggatgaagga
      481 ctcgcggcta tctcgaagaa gcccgaagac cgcggcaagt tccgcacgcc gacgctgcgc
      541 aacatcgccc agaccggtcc ctacatgccc gacggctcgc tcaagacgct cgatgatgtc
      601 gtgacgtttt actaccgcgg cgcgccgacc ggcaaggtgg acggtctgac tcccgacatc
      661 cagccgctgc tcggccaaag cttctccgag attcccgatc tcgtggcctt cctcgaatcg
      721 ctgacgggag aaccgccgca gatcacgcgg cccgagctgc cctagaagct atttccaaag
      781 tgtccaagcg gctccggtca gcagcgcgat gcccaagacc aggatgatcg ctgagacgat
      841 cttgcggaac gttgtttccg gaatccgccg cagcaagcgc tcgccggcga ttgtccctgc
      901 gacaacgccg acggtcgccg ccgccacgta gggccaggct ttggcgatgt cgctccactg
      961 cgtgagaaaa tagacgggca tccgcgcgcc atcgaccagc aggccgaccg cggtggcggt
     1021 ggcgacgaag gcgtctttgc ggatatcgaa gcccatcagc gcggcagagc ggatgccgcc
     1081 ttgattgccc accaggccgc caaacgcgcc agatagcact ccggcgaccc atgcagtcca
     1141 gccggagaac cgcatcttgc tggcgtagcc tgtcacgccc atcacgccgg caaaaaccaa
     1201 cagcacgccc ggcaccgcgg tgagcagcgg gctcgccagg aaggcgccga ggaacgcgcc
     1261 ggccaatccg cccgccgcgc tcgtcaggcc gaagctccac agcacgcgcc aattgaagtg
     1321 cttgcgcagg atccagtaac gcagcgcggt ggcaatgaag tggggaatcg aaatcaccgc
     1381 aacggcgacc ttcatctcga cctgcaggcc gagcagcggc gtcagcagac tgccgatgcc
     1441 gaatcccgcg accgacgcaa tggctccggc ggccagcgcc acgatcagca caagcagcag
     1501 ggccagccag aagctgagca ttgccacaac tcgttacttt ggccaatccg cttgcggatc
     1561 gaagttgatc atcgccgaag cgggccgctc gggaatatgc gggtggaggt acggtttatc
     1621 cgacgcctta tggcacgaat aacatccctc gacgatgtac ttatatgtcg gctcgaactt
     1681 tgccgcgtct tgctgcttga tcacttcgcc catttgcatg tagggtgagt tttcgatcga
     1741 tttcagaatg tcttccagct tcacctcttt gccggcgttg tctttccgga ccgggatgat
     1801 ccgcaccgcc cacttcatgt gcgccagcga ttccttccag tagaagtcgg ccagcggcca
     1861 gttctgggct ttgccagcga accacaggtt ggagaaatgg taatccacgt cggacatcgc
     1921 gtgtgattga tctggcacga tctttttgag gcgctcgacc tctgccgcca gcgcagctaa
     1981 atcaaggggc ggagccttcg ccgactgttg gccgtaggcc gcggagccaa ccgtgcccag
     2041 atcggaactc attggacgaa tgagaatgcc gagcgcgccg ccggtcagaa acgccgtgag
     2101 gatgaaaaga gctgtggagc gactcatgct gacctcctcc gaaatgtggc aggccgaaag
     2161 ataacgccct cgctatcgac tttcaatatc gaaagtcgat attatggccg gtcgatttga
     2221 ccgtggaacg gacaaagtgt cccgtgctcc tgcgcttggc tgctgccgtc catccgccga
     2281 acctgaaggg tacgagaatt gctgattcgc attccgtgca tcagccgcga atcgcgaggt
     2341 gacgtatgcc agctccgcgc cgcgagttca tgacacactc tgccgccctc gccgccgcag
     2401 cgttagcgcc gaggagtcca atttggggaa aggagccagt tgtggaagcc ccttcagcgg
     2461 agaaatcggc tgaagcgatc tccgcgcccg aagacttgat gcgcgaacac ggggtgctga
     2521 accgcgtgct gttgatttac gaggaaggcg ttcgccgcct gcggaagggc gaggaagccc
     2581 cgccggagat ttttcagcgc ggcgcgcagc tcgtgcggcg attcgtcgag gactaccacg
     2641 agcaactcga gcagaacttc atctttccgc agttcgagaa gcgccaagcg cacgccctgc
     2701 tggtgcgtgt cctccgccag cagcacgatg cgggccgcaa gctgacggat gccatccagc
     2761 gggcttccgc cccggaccag ttcgtcaaag ctcctgcccg cgaacagctc gcgcggacct
     2821 gcgaggcttt ttgccgcatg taccgtccgc acgaggcgcg ggaggacacc gttctgttcc
     2881 ccgcgctcta caagatcgtg cccgcgacgg agatcaagga tctgggcgag cagttcgagc
     2941 agcaggaaca caagctcttc ggtgaagagg gttttcaaaa aacagtcgaa caggtcgccg
     3001 aaatcgaaaa gctgctcggg atttacgatc tggccgtctt cacgcccaag tcgccggtga
     3061 ctgccgatgg ctctcgttaa caaactcctc gtcacggaca cccccgctgc cgcgatcctg
     3121 gtccgtgtgc tggtcggcgc ggtcttccta tcggaaggaa ttcagaaatt cctcttgccc
     3181 gctgccgttg gcgccggacg gttcgccaag atcggcatcc cctcgccaga gttcacggcg
     3241 cctttcgtcg gctgcttcga aatcgcctgc ggtgttctgc tgatcctcgg gctgttcacg
     3301 cggctgggtg cgataccgct gatcgtcatc atgctcgttg ccatctcgac gacaaagatc
     3361 ccgatcctga tgaacgaggg tttctggaag gcggcccacg agtcgcggac tgattggtcg
     3421 atgctgctcg gctcgatctt tctgctgatc gtcggcgctg ggccgtggtc gctcgatgcc
     3481 tggctggggg cgaacgcgcg ggctagatct cgatgaagcc gatgaccttg ccctcttcct
     3541 tccggccttt cttccgctcg gccatcgcgt ccttgtcatc gacaatctcg gaggccagct
     3601 cgaccagctt ctcgcgggct tcagcgagca gcttccgtcc gcgggcggtg gtccgaaagt
     3661 tcttccgccg cttgccgcca accacctcgt ccttggcctt cacgaagccg gattcctcca
     3721 ggccgtgcag cacgggatag agcgttcccg ggccgatttt atagccgtgg tgctgcaatt
     3781 cctccattaa ttcgacgccg caaacgggct gtttatcggc gtgatacaga atgtgcaggc
     3841 gcacgaagcc ggtaaacagg tgcttcagga cgctgctggc cataatatcg aaatccgata
     3901 ttgattatcg atcggaggaa agtagaatca agcctccgtc gcgatttcgt tctcagcagc
     3961 atcttggatt ccgcgacgac aattgacaag cccgcccgca ccgtaattct cgacagccga
     4021 tgaatctcat cgccgccgct ctccgccggc cgttgaccgt gatggtcgcc atcgcggcca
     4081 tcgccttcgg cttcacgctg gcggtgggca agagcgtttg cgaccaggcg ggaattccgt
     4141 atcccgcggg cctgcccaag gggatggagg tcgatatttt cccggccctt aacctgccgg
     4201 tggtctatgt ctgccagccc tatggcggca tggacccggc ccagatggaa ggctacctga
     4261 ccaacttcta cgagtaccac ttcctctaca tcagcaacat tcaccacgtc gagtcgcgaa
     4321 acgtgcaggg cgtggccctg atgaagctgc acttccatcc gggcacgaac atggcccagg
     4381 cgatggccga gacgatcagc tacgtcaacc gctcgcgggc cttcatgccg ccggggaccg
     4441 tctcgccgtt cgtgatgcgg ttcgacaccg gcagcgtgcc ggtcggctat ctcgtgctgc
     4501 ggagcgacac ccggaccgtc gccgagatca acgacctggc cctgttctcc gtgcggccga
     4561 cgttcgccgg cctgccgggc gtttccgccc cgccgccctt cggcgcgggc gcgcgcagtc
     4621 tcgttgtcac cgtcgatccg cagcggctgc agtcgtatgg catgtcaccc gacgaagtga
     4681 tcgtcgccgt cacgaaaggg aacacgctcg gtccgtcggg caatgtgccg atcggcgaca
     4741 agttcgctat cgtgcccgtg aattcggtcg tcaaagacgt caaccagatg ctcagcatcc
     4801 cgatccgcac cgggccggat gccgtttatc tgcgcgacat cggcaccgtt gccgacgcgg
     4861 ccgatgctcc caccgggtac gccctgatca atggccggcg ggccgtctat atcctggcca
     4921 ccaagcgggc cgatgcttcg accctctcgg tcgtgaacac gatcaagcgg gccttgcccg
     4981 agatgcaggc cgccctgccg ccggacatca aagtcagctt tgagatggac cagtcaccgt
     5041 acgtcacccg cgcggtcgtc ggcgtgctga ccgaagggac gctcggcgcg gtgctcgtcg
     5101 gtctgatggt gctcatcttc ctgtgggact ggcgcagcgc cctggtcgtc gttctcaata
     5161 ttccgctggc cctcatggcc tcagtcctcg ccctctggat caccggctac acgatcaacg
     5221 tcatgacgct gggcggcctg gcgctggcga tcggcgtgct ggtcgatgaa gcgaccgtcg
     5281 aagtcgagaa catccatacg cagatgcggc atacgggctc gattgcccgc gcggtgcgcc
     5341 tgggcaatgc cgttaccgcc gtgccgcggc tcttggcgat gctttgcatc ctggcagtct
     5401 ttgtgccggc cttcttcatg gaaggcgcgg cccaggcgct ttttgccccg ctggccctcg
     5461 ccgtcggctt cgcaatggtc gcctcctacc tcctcagcag cacgttcgtg ccggtcgttt
     5521 cagtctggct gttgcgtcac gctcatggcg ggcaaggcag agggaattct tctcccctct
     5581 cccctcgtgg gagaggggcc gggggtgagg gggc
//
LOCUS       DASUGB010000873        13793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1208372, whole genome shotgun sequence.
ACCESSION   DASUGB010000873 DASUGB010000000
VERSION     DASUGB010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208372"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFV87_16590"
     CDS             complement(<1..1007)
                     /locus_tag="VFV87_16590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105440.1"
                     /translation="MEQTTVKPKGSAAPAAAAADASSPLVSHPSMPLGANGVIWTLWL
                     TYGAFYFCRTNISSAAAGMKLPLAEGGLALSGEEVGWILASLKIAYGLGQLLNGQLSE
                     RISPRVMLAIGMFGSAALNVLFGLSAGFWFLLFVWATNGFCQSLGWTPCIRVAANWIP
                     VQRRGHAIGIIGTGYQITLGLTYFIASQAAEHLGWRGALYVPATLLAAAGVVMLLFLR
                     EMPPDHEQLEHGQPRPARGESRISFIDGMYWTLYNPALWLMGLALGLLNACRYGFVDW
                     GVTHLMETQHVGVGKAGLQYVVIAIGATAGAYLAGWATDRFFGSRRAPVICLLMVLLG
                     AL"
     gene            1229..1351
                     /locus_tag="VFV87_16595"
     CDS             1229..1351
                     /locus_tag="VFV87_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105441.1"
                     /translation="MADYVEVPEGTMVRDLLQREAPSGMAENYVMAGAGFNCAA"
     gene            1587..2339
                     /locus_tag="VFV87_16600"
     CDS             1587..2339
                     /locus_tag="VFV87_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5105442.1"
                     /translation="MKIVVIGGSGLIGKKLIPLLRARRHEAVSASPSSGVNTITGEGL
                     ATALGGADVVVDVTNSPSWEDKAVLEFFETSTRNILAAEASVGVKHHVALSVVGADCI
                     PDSGYMRAKVAQEKLIQASRVPYTILRATQFYEFLAAIAGEGAGTVRVSTAPMQPMAA
                     DDVAAALADVAVGTPAGGILEVAGPESLSIAAFVRKALAASGDSRRVVADPLASYYGA
                     ALDDLGLRPRGANPRIAPTRFEAWLDRSVARA"
     gene            2432..2911
                     /locus_tag="VFV87_16605"
                     /pseudo
     CDS             2432..2911
                     /locus_tag="VFV87_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
     gene            complement(3000..3872)
                     /locus_tag="VFV87_16610"
     CDS             complement(3000..3872)
                     /locus_tag="VFV87_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5105443.1"
                     /translation="MNRRRFLRDGSLLLLGAGVTGSPLASHAATEPQHRVRIGLLTDL
                     HYADKPPVGSRHYRETLTKFAEAAGEFQKAKPDFVVELGDLIDAADSAKAEKKYLATI
                     NKEFAALPGDKHYVLGNHCVATLTKDEFLEGVARSKPHYSFDAGGFHFVILDACYRSD
                     GESYSRGNFQWTDTSIPVEQIEWLKADLQAAAGKAVVFVHQRLDVKNDYGVKNADQVR
                     QVLEESGKVLAVFQGHSHKNDLKDIGGIHYCTLVAMVEGSGPENSGFSLMDICDGGTI
                     RLTGFRKQSSYNWQ"
     gene            complement(3889..4311)
                     /locus_tag="VFV87_16615"
     CDS             complement(3889..4311)
                     /locus_tag="VFV87_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX5105444.1"
                     /translation="MAALATADDAKDEAIKKDRQLIEGTWRVVALEVDGNKASDDDAK
                     KITVVNGPDGTWTLFSEGKEVGKGTNSFDPTKKPKTIDFTQTEGEGKGNLYLGIYELG
                     ENTRKLCFAPPGKDRPREFISMPGTDHILVTFERVRDK"
     gene            4491..5324
                     /locus_tag="VFV87_16620"
     CDS             4491..5324
                     /locus_tag="VFV87_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5105445.1"
                     /translation="MRLQIRHLACLAIGLSIGELRASGEEPASAPIKKLILPGEAFLV
                     QDRPAFVLLPPEKERSTPQPWIMYAPTLAGYPDEHEKWMHEQFLAAGVAVAGIDIGES
                     YGSPPGRKLFTALHDELTKNRGFANKPCLLGRSRGGLWVTSWACDHPEQVAGIAGIYP
                     VFDLRTYPGVAKAAPAYGLKADELEAKLSENNPIERVGVLAKAKVPVFIIHGDDDKVV
                     PLKENSAKLAARYEAAGAKDTVTLVVAKGQGHNYWEGFFRCPELIEFAIARARSGAAV
                     P"
     gene            complement(5441..6619)
                     /locus_tag="VFV87_16625"
     CDS             complement(5441..6619)
                     /locus_tag="VFV87_16625"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105446.1"
                     /translation="MQVFLCGGPSQHDTFDPKPDGPAECRPFESIQTVLPGVRVTEFF
                     PRLAAIFDEFSVVRSVAHGDGSHNHSVHWLQTGRYPDKLVFGQNQFPATGAVIAKYRG
                     ANSPGVPPNVAIPEGFYYSTASYLGQRYNPMEVGDPNQPDFRVSNLQLIDGITTNRLD
                     DRLRILRGLDRFRRDVDTHGRFAAMDAFQISAYDMISGPAVRRAFDISGEDSRLRDRY
                     GRTRLGQACLLARRLVEAGVTFVNVKDYEFFEWDLHGTVGGPGIEGTKTKGPHLDAAL
                     SSLILDLKERGLLDRVLVQVFGEFGRTPNINATAGRDHWGSVFTALFAGGGLRHGQVI
                     GSSDAKGAMPQDRPLAPTDLLATMYRFLGISTDLAPIDLSGRPIPLLPSGEPILEMFG
                     "
     gene            6584..6751
                     /locus_tag="VFV87_16630"
     CDS             6584..6751
                     /locus_tag="VFV87_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105447.1"
                     /translation="MLAGTTAQEDLDDGGVFASILRWGALPQRIDQARSAGCQRTKSQ
                     KLPTIDVVAAS"
     gene            complement(7539..8339)
                     /gene="nuoB"
                     /locus_tag="VFV87_16635"
     CDS             complement(7539..8339)
                     /gene="nuoB"
                     /locus_tag="VFV87_16635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX5105448.1"
                     /translation="MFNVIRERLQQGYRTAAYPDQPPPPLPERFRGLPLIDSSKCPAP
                     CEECCSVCPTDALQFRDKQLQLDMGRCLFCTDCLEVCPAGAIQFTNDHHLASRTREGL
                     VRTDGDLQQLAAALDERLKGLFGRSLKLRQVSAGGCNACEADVNVLNTVVFDLSRFGI
                     QFVASPRHADGLLITGPVTENMRIALQKTYEAVPAPKLVIAVGACAISGGPYIDHAEA
                     HNGADSVLPVDLYVPGCPPSPWTILDGLLRLLGRIAENERGPALALRR"
     gene            complement(8358..9827)
                     /locus_tag="VFV87_16640"
     CDS             complement(8358..9827)
                     /locus_tag="VFV87_16640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5105449.1"
                     /translation="MPHLDFTRFRETIVSRANGTTRLVALFGQPREGNGVRLYAVLAD
                     ETEGLLSPLTCDRDSDEFPSLTPDCPQAHNFEREIAEQWGLVPRGHPWLKPLRFHHSY
                     RPGHDAWGRAVGEQILPCVTEHFQVAGSEIHEVAVGPVHAGVIEPGSFRFQCHGEHVF
                     HLEIHLGYQHRGVERALVGGPNKRTIHYMETLAGDTSIGHALAYCQAVEGLAGVPPSL
                     RAQAIRGIALELERLACHTGDLGALADDVGYLPTANYCGRLRGDFLNASGAICGSRFG
                     RGLVRPGGVTFDIEPAMATQLSDRLKPALRDVGGAAKLLWESQTVMARFENTCTLKPA
                     DAAALGIVGPAQRACGIERDVRQDFPAGIFRSAQIPVATWPTGDVFARAYVRGLEIER
                     SGAFVLEHLHTLHPGPLRASMGPLAANSLVVSLVEGWRGRICHVAITGPDGRFVRYQV
                     VDPSFHNWIGLMMALRDQQISDFPLCNKSFNLSYCGHDL"
     gene            complement(9900..11342)
                     /locus_tag="VFV87_16645"
     CDS             complement(9900..11342)
                     /locus_tag="VFV87_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5105450.1"
                     /translation="MAELLVCLPVAWAALAFAVPSNRWRPWVLPAGGLVQLVLAARAL
                     QETNLTGWDNWLNLDPLGRIFLGLISVLFFLCACYAPGYLAQRSQRDNRVLCACLLLS
                     LSMMTLIILSHHLGLMWVAMEATTLISAPGLYFNHNPRSLEATWKFLLICSVGIALAL
                     MGSLFLAYAALHSGLKTSLLFDDLVRQAPLLSRPWMHAAFVFLFVGYGTKMGLAPMHT
                     WKPDAYGEAPGMIGALLAGGITNCAFLCILRFVHITNAAHDAQFAHRMMVFVGLLSIG
                     VGAAFMVRQRDFKRMLAYSSVEHMGILVLGIGIGGAATWAALLHAINNGLTKGILFLS
                     AGNIHRAYETKLTADVRGVIRRLPVSGWLFLLGFLAITGSPPFAPFVSEFSLLAAAFR
                     QGRFVEGALFAIMLLVVFIGFGATVTSMVFGAPSPAATSTAIHDSAATCWPIVAFAVL
                     VLVLGVYLPPPLATMLRDAAMFLEGAPSPG"
     gene            complement(11360..12028)
                     /locus_tag="VFV87_16650"
     CDS             complement(11360..12028)
                     /locus_tag="VFV87_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105451.1"
                     /translation="MNLTTIDPFLVAVVLLNFFLLGTGRLKALIFAVALQGAILGIIY
                     PLTHQGLHAAGTSVVSLRLLALAAAMIAIKGLVMPRMLTYAMREARVSWRIEPFLGLT
                     TSLLLGAIGTGLALALSRTLPLRSEHASHLLVPASLSTVFTAFLILTTRREALTQVLG
                     YLVLENGIFIFGLLLVDALPLIVEVGVLLDLFVGVFVMGIIIHHVNRQFTASTSDHLS
                     ALKE"
     gene            complement(12042..12965)
                     /locus_tag="VFV87_16655"
     CDS             complement(12042..12965)
                     /locus_tag="VFV87_16655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5105452.1"
                     /translation="MLTILHNVLHLLLVGTLPPLLLGIINRTKAFFGGRAGPPLLQPY
                     YDLWRLLRKGSVQSTTTTWIFRFGPVVSLVTALLAALFIPLGHNTAPLGFAGDLLLFA
                     YLLALGRFFLVLSALDTGSAFEGMGAAREVTFACFAEPAMFFCLLVLAKLTGSLQMAE
                     LFRGSLFSGTFAAAAPLLLVLMSWFLLMLAENCRIPFDDPNTHLELTMIHEVIVLDHS
                     GPMLALLQEAAAIKLFVFASLLVRLAVPLDPQNAWVSYPAFAASVCVVAVLLGIVESV
                     MARLRMTLVPHVLTAAAVIAAFGFVLLVTLP"
     gene            complement(12976..>13793)
                     /locus_tag="VFV87_16660"
     CDS             complement(12976..>13793)
                     /locus_tag="VFV87_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105453.1"
                     /translation="ATAICGLPPLNGFVSEFVIYTGLLHTLGLKQEPGFPVVAFAVPA
                     LALMGALAVACFVKVFGVVFLGVGREPHPQAACESPWTMLLPQFALAGCCFAIGLGST
                     LVAPLLDKVTAIWGGQSLAEAQPLVQAAALPQVSVLAVALIAALAIGTAVLNWRMRTQ
                     PLGWTETWGCGYTRPTPRMQYTASSFAQFLVALFGWALQPTAHQPRLTSLFPQSAHFE
                     SHVPEVVLDRLVMPAFHLAARILFWFRLMQQGSVQIYLLYVFAILVLLLLVFR"
BASE COUNT         2849 a         4211 c         4393 g         2340 t
ORIGIN      
        1 agcgcgccaa gcagaaccat cagcaggcaa atgacggggg ctcggcggct gccaaaaaac
       61 ctgtcggtcg cccaaccggc caaataggcg cccgcggtgg cgccgatcgc gatgacgacg
      121 tattgcaggc ctgctttgcc cactcccacg tgctgcgtct ccatcagatg cgtgacgccc
      181 caatcgacaa acccataccg gcaggcgttg aggagcccca gcgccagacc catgagccac
      241 agggccgggt tgtagagcgt ccaatacatg ccgtcgatga aggagatccg cgactcgccg
      301 cgcgctgggc gcggctggcc gtgctccagt tgttcgtgat ccggcggcat ttcccgcagg
      361 aagagcagca tcaccacccc ggcggctgcc agcagggtcg cgggtacata tagcgcgccg
      421 cgccagccga gatgctccgc cgcttggctg gcgatgaagt acgtgaggcc gagggtaatc
      481 tgatagcccg tgccaatgat gccaattgcg tgcccacggc gctggacggg aatccaatta
      541 gcggcgacgc gaatgcacgg ggtccagccg agcgactggc agaagccgtt cgtggcccag
      601 acaaagagca ggaaccagaa tccggcactg aggccgaaca gcacgttgag ggcggccgag
      661 ccgaacatgc cgatcgccag catgacccgc ggactgatgc gctccgacaa ctggccgttg
      721 aggagttgtc ccaggccata ggcgatttta agcgacgcca gaatccagcc gacctcctcc
      781 ccggaaaggg ccaagccgcc ttccgccaga ggcaatttca ttcccgctgc cgccgaggag
      841 atgttggtgc ggcagaagta gaaggcccca tacgtcagcc acaaagtcca aatcaccccg
      901 ttggcgccga gcggcatcga gggatgggac accagcggag acgacgcgtc agcagcggcc
      961 gcggcaggtg cggcgctacc ctttggcttg acagtcgtct gttccatcac gtcatccagg
     1021 ccaggcggaa cccgtgtagg cgacgggtca gtgtatcatc ggggccggag gattgctccc
     1081 cggcaaggta gaacacgtgc ttcccgtcct ggatcagccg ccgattcgcg gcaggaaccg
     1141 gtgatccgaa ggtatcggtc cggcctgcgt ggaccaataa cagctcaggt caagcagctc
     1201 tcggatactg cggcagaacg gcggcggatt ggccgactac gtcgaggttc ccgaaggcac
     1261 aatggtccgc gacttgctcc agcgcgaagc gccctcaggt atggccgaaa actacgtgat
     1321 ggccggcgct ggattcaatt gtgccgcgta gttttcgtgc cttctctcgc ggtcagacac
     1381 ctctgatgaa acgtgccgcg gagctccagc accgggtgta aaatcctggg ctgggtgact
     1441 cattcttgag gagtaccgga gcacgagcaa aaagcgcatg tggaagcggt acgaggcttc
     1501 gttcaaagcg cgtttgcccg agaaatagaa agcgatggaa ggcttgacct tcacccatta
     1561 tccctcacag gaaaaggatc gcaaacatga agatcgtggt aatcggcggc agcggactca
     1621 tcggcaagaa actgatccca ttgctgcgtg cgcggcggca cgaggcggtg tcggcctccc
     1681 cttcgtcggg cgtcaatacc atcaccgggg aagggctggc cacagccctt ggcggtgccg
     1741 acgttgtcgt cgatgtgacg aattcgccgt cctgggagga caaggcggtg ctggagtttt
     1801 tcgagacatc cacgcgcaac atcctcgccg cggaagcgtc cgtcggcgta aagcatcacg
     1861 ttgccctgtc ggtcgtcggg gccgactgca ttcccgacag cggctacatg cgggccaagg
     1921 tcgctcagga aaagctgatc caggccagcc gcgttcccta cacgatcctc cgcgcgacgc
     1981 aattctatga gttcctcgcc gcgatcgccg gggagggcgc tggtaccgtc cgagtctcca
     2041 ccgcccccat gcagccaatg gcggcagacg atgtcgcggc tgctctggcc gatgtcgccg
     2101 tgggaacgcc ggccggcggc attctggagg tggccgggcc tgagtcgctg tcgattgctg
     2161 cattcgtaag aaaagccttg gccgcgagtg gagacagcag aagggtggtc gccgatccac
     2221 tggccagcta ctacggcgcg gcactcgatg acctcggcct ccgaccccgg ggagccaacc
     2281 cgcgcattgc gccgacgcga ttcgaagcct ggctggatcg ctccgtggct cgggcgtgat
     2341 tcggccgttc gtcggtggac ggaggctgac acggcgttgc ggagaaccat aataggatcg
     2401 cagcgcgatt attgctcccg gaaagtccgc aatgccgagt cagcccaagc acagcgcaga
     2461 gtcacttcgc ctggaacaag ccgcgaagcg cgagaacaac tggcagcggc gggggcctta
     2521 tctcgccgag cggcagtggg gcagggtgcg cgaggattac tccgcaattg gcgacgcctg
     2581 ggactacttc tcgcacgatc aggcccgcag ccggtcgtat cgctgggggg aagatggcct
     2641 cttcggcatc tgcgatcgcc actgccggct ttgcttctcg gttgcccttt ggaacggtcg
     2701 cgacccgatc ctcaaggagc ggctcttcgg actgacgggc cctgagggca atcacggcga
     2761 agacgtgaag gagtgctact actatctcga ttcgacgccg acgcactcct acatgaaggc
     2821 cttgtacaag tacccgcagg gggagtttcc ctacgcgcgg cttgtcgagg agaatcggcg
     2881 tcgcggcaag gccgacctgg agttcgagct caccgtcacc tggatgcgat attcgggctc
     2941 agcagccgac gcattcacgg caacgaggcc cagaatcaga agcggaattg agcgaggcat
     3001 cattgccagt tgtagctgct ctgcttgcgg aaacctgtca gccggatggt accaccatca
     3061 cagatgtcca tcagcgaaaa gccgctgttc tccgggcccg atccttcgac catcgcgacc
     3121 agcgtgcagt agtgaatgcc gccaatatcc ttgaggtcgt tcttgtggct atggccctga
     3181 aagactgcca aaaccttgcc tgattcctcc aggacctggc ggacctgatc cgcgttcttt
     3241 acgccgtagt cgtttttcac atccagccgc tgatggacga acacgacggc cttcccggcc
     3301 gcggcctgga gatcggcctt gagccattca atctgctcta ccgggatgct ggtgtccgtc
     3361 cattggaagt tgccacggct gtaggactca ccgtctgaac ggtaacaggc atccaggatg
     3421 acgaagtgga atccgccggc atcaaaggag tagtgaggtt tgcttcgggc cacgccttcg
     3481 agaaactcgt ccttggtgag cgtggcaaca cagtgattgc ccaggacgta atgcttgtcg
     3541 cctggcaggg cagcaaactc tttgttgatc gtggccagat acttcttctc cgccttggcg
     3601 gaatcagcag catcgatcaa atcgccaagc tcaacgacaa aatctggctt ggccttttgg
     3661 aactcgccgg ctgcttctgc aaacttcgtc agtgtttccc ggtagtgccg tgatccaact
     3721 ggcggtttat cggcgtagtg caggtcggtg agcaggccga ttcgtacgcg atgttgcggc
     3781 tccgtggcag cgtggctggc gagcggactg cccgtaacac ccgctcccaa gagcagaagc
     3841 gaaccatcgc ggagaaatcg gcgtcgattc atgtcctaac tctcgcaatt acttgtccct
     3901 gacccgctca aatgtcacga ggatatgatc cgttcccggc atggaaatga actccctggg
     3961 acggtccttg ccgggcggcg caaagcacag tttgcgcgtg ttctcgccca gttcataaat
     4021 ccccaggtac agattgccct ttccttcgcc ctcggtctga gtgaagtcga tggtcttggg
     4081 ctttttggtc ggatcgaagg aattggttcc cttgccgact tccttgcctt ccgagaacag
     4141 ggtccaggtt ccgtccgggc cgttgacgac ggtgatcttc ttggcatcgt cgtccgacgc
     4201 cttgttgccg tccacttcca gggcgacgac acgccaggtt ccctcgatca actggcggtc
     4261 cttcttgatc gcttcgtcct tggcgtcatc agcggtcgca agcgcagcca ccgcgaacaa
     4321 cgccaggcct agcgccgatg ggccaattgt cctgtttatc attttgaatc tcccgtgctg
     4381 attttgcggt tggccagggg ctgtcgagca cgcgcttgcc aagccacaat gcagtggctc
     4441 acaagaacca gtctatcgca tcgtcttcgt tccaagtgct ggaggcaatc atgcgactgc
     4501 aaatcaggca ccttgcctgt ctggcgattg gtttgtcgat cggtgagctg cgcgccagcg
     4561 gtgaagagcc tgctagtgct ccgatcaaga agctgatttt gccgggcgag gcatttctcg
     4621 tgcaggatcg gccggctttc gtcctgctcc cgccggagaa ggaacgcagc actccgcagc
     4681 cgtggatcat gtatgcaccc accttggccg gatatcccga cgagcacgag aagtggatgc
     4741 acgagcagtt cctggcggct ggagtcgccg tggcgggaat cgatatcggc gagtcgtacg
     4801 gcagcccgcc gggacgcaag ctcttcactg ccctgcacga tgaactgacc aaaaaccgcg
     4861 gctttgccaa caagccctgt ctcctcggtc gcagccgcgg tggattgtgg gtcaccagtt
     4921 gggcctgcga ccatcccgag caggttgcgg gaatcgccgg gatctatccg gtgttcgatc
     4981 tgcgaactta tccaggcgtc gccaaggcag ctccggccta cgggctgaag gccgacgagc
     5041 tagaagcaaa actcagcgag aacaatccca tcgaacgcgt aggcgtattg gccaaggcca
     5101 aagtgcccgt gttcatcatc cacggcgacg atgacaaggt cgtgccgctc aaggaaaact
     5161 cggccaagct tgccgctcgc tatgaggctg ctggcgcgaa ggacaccgtg acgctcgtcg
     5221 tggccaaggg ccagggccac aactactggg aaggcttctt ccgttgtccc gaactgatcg
     5281 agtttgccat cgctcgggcc cgcagtgggg ccgccgttcc atgatcctgc acatcgacat
     5341 ggacgcgttc tatagctccg ttgattcgat gcgcgcccaa ccaaaaatag gatcttcgac
     5401 cggcggctag tgctagtcgg tgctgacgtt cattcatttg tcagccaaac atctcgagga
     5461 tgggctcgcc gctgggcagc aatgggatgg gcctgccgga cagatcgatt ggagccaggt
     5521 cggtcgaaat gcccaggaag cggtacatcg ttgccagtaa atcggtcggt gcgagcggac
     5581 gatcctgcgg catggccccc ttggcatcgc tggaaccaat gacctggcca tggcgtaacc
     5641 cgccaccggc gaacagcgcc gtaaagacgc tcccccagtg atcgcggccg gcggtcgcat
     5701 tgatgttcgg cgtgcgccca aactctccaa acacttgcac caggacgcga tccagaagac
     5761 cacgttcctt cagatcaaga atcagactcg acaacgccgc gtcgagatgc gggcctttgg
     5821 ttttcgtccc ctcgatgcca ggcccgccga ctgttccatg caaatcccat tcaaagaact
     5881 cgtagtcttt cacgttgacg aatgtgacac cggcttcgac cagccgtcta gctagcaagc
     5941 acgcctgccc cagccgcgtg cgtccatagc ggtcgcgcag ccgcgaatct tcgcccgaga
     6001 tatcgaacgc gcgccgcaca gcggggccgg aaatcatgtc gtaggccgaa atttgaaaag
     6061 catccatcgc tgcaaaccgg ccatgcgtgt cgacgtcccg tcggaagcgg tccaggccgc
     6121 gcagaattcg caaccgatca tcgaggcgat ttgtcgtgat gccgtcgatc aactgcaaat
     6181 tcgagacgcg gaagtcaggt tgattcgggt cgccgacttc catcgggtta tagcgttgac
     6241 ccagatagga agcggtgctg tagtagaacc cttcaggaat ggccacattc ggtggaacgc
     6301 ctggggaatt ggctccgcgg tatttggcga tcacggcgcc ggttgcagga aattggtttt
     6361 ggccgaagac cagtttgtct gggtagcggc cggtctgcag ccagtggacg ctgtgattgt
     6421 ggctgccgtc gccatgcgcg acggatcgga ctaccgagaa ctcatcgaag atggcggcca
     6481 atcgcgggaa gaattccgta acgcggaccc ccggcaacac ggtctggatt gactcgaacg
     6541 gccggcattc cgctggaccg tccggcttgg gatcaaatgt gtcgtgctgg ctgggaccac
     6601 cgcacaggaa gacctggatg acggcggtgt ctttgcgagc atcctgcgct ggggagcgct
     6661 gccgcaacgc atcgaccagg ctcgctccgc cggctgccag cgaaccaagt cgcaaaaact
     6721 cccgacgatt gacgtcgttg cggccagctg agaagagact caacattgca gcagctattc
     6781 ccttgacgtt gcgcgatcag gtcgttctgc agccgattct gtgcgatgga cacgtgaaaa
     6841 gatagccatc gacgggccgg caacggggaa tctggcagcg cggaaaccgc tccggacgtc
     6901 gttgagcgcg ggagattcgg ccgaccccac tcccgaacct cgcaatgaaa tgtgcgaagc
     6961 agggtgtcct gctcctcgac gtaggtcgac tgcgtcaaga tccagccggc gtatgccttc
     7021 gcgatctgga aatgccgatc gagccgacgc tcagctgtcc gcacccactc tccagattcg
     7081 gaagtgtggc tggtcagaaa attgagggcg gcctcgtgca acctccttcg gtgtgcctca
     7141 ccgaagcgca ccgggcaacg agccgatctg cgtcgaggag agcgacggtc ggccgtgttt
     7201 ggatcgcggt attgactcag ggatcgcgcc ttgagagggt acgattccac cagtccgcaa
     7261 acgatttgac ccatcgttcc caccgggatt tgtgtggacg atgcccggcg ccttgggcgc
     7321 gcagctcgct ggcagccgcg accaaagcgt catcaattgt catctcccca tgccatccac
     7381 agtggcaggt gatatgcatc cgttttcgcg gcgcatgctt cttggcttta agtgcacggc
     7441 cgcaagcagg gcaagtacca aggagaacgt gtggcaagtt ggcctcctgg tcgcgctgtc
     7501 gcaggaccgc attgccgaaa tggaccggcg aggagccgtt accggcggag agcgagagct
     7561 gggccgcgtt cattttcggc gatacgccct aatagccgca acaatccatc cagaattgtc
     7621 caagggctag gcgggcaacc cgggacatac agatcgaccg ggaggacgct gtccgcccca
     7681 ttgtgggcct ccgcgtggtc gatataggga ccgcccgaaa tagcgcacgc tcctacagcg
     7741 atgaccagct tgggcgcggg cacggcctcg taggtcttct gcagcgcgat ccgcatgttt
     7801 tccgttaccg gaccggtaat gagaagtcca tcggcgtgac gcggcgaagc gacgaactga
     7861 attccgaacc ggctaaggtc gaagaccacg gtattgagaa cgttgacgtc ggcttcgcag
     7921 gcgttacagc cccccgcgct gacctgccgt agcttgagag agcggccgaa gagacccttg
     7981 agccgctcgt cgagggccgc ggcaagctgc tggagatcgc cgtcggtgcg gacgagtccc
     8041 tcgcgagtcc gtgaggcgag gtgatgatcg tttgtaaact gaatcgcacc ggcgggacaa
     8101 acctccaggc aatccgtgca gaacaggcac cgccccatgt cgagttgcag ctgtttgtcc
     8161 cggaactgga gggcatcggt gggacagacc gagcaacact cttcacaggg tgcagggcac
     8221 ttcgacgagt cgatgagcgg caggccacga aatcgctccg gcagcggggg gggcggctga
     8281 tcgggatagg cggcggtgcg atagccctgc tgcagccgtt cgcgaatgac attgaacata
     8341 ggagtaccga cgcggagtta caggtcgtgt ccgcagtacg agagattgaa gctcttgttg
     8401 cagagtggaa agtcggaaat ctgctggtcg cgaagggcca tcatcagacc gatccagtta
     8461 tggaaggacg gatcgacgac ctgatagcgg acgaagcggc catccgggcc ggtgatcgcg
     8521 acgtggcaaa tcctgccgcg ccaaccttct accagtgaga ccaccaggga attggccgcc
     8581 agcggcccca tgctggcacg gagcgggccg ggatgcaggg tgtgcaggtg ttccagcacg
     8641 aacgcgccgg accgctcgat ctccagtccg cggacatacg cccgggcaaa tacatcgccc
     8701 gttggccagg tggcaaccgg aatctgagcc gagcggaaga tgcccgcggg aaaatcttgg
     8761 cgcacgtcgc gctcgatgcc gcaggcccgc tgcgccgggc cgacaatccc cagggccgcg
     8821 gcgtcagccg gcttcagggt gcaggtgttt tcgaaacggg ccatgaccgt ctggctctcc
     8881 cacagcagct tcgccgcgcc gccgacgtct cgcagcgcgg gcttcaggcg atcgctcaac
     8941 tgcgtggcca tagccggctc gatgtcgaac gtcactccac cgggacgcac cagcccgcga
     9001 ccaaaacggc tgccgcagat tgcgcccgaa gcattcaaaa aatcgccgcg caagcgcccg
     9061 cagtagttgg cggttggcag gtagccgacg tcgtcagcca gggcgccgag atcacccgtg
     9121 tggcacgcca ggcgttccag ttccagagca atcccgcgaa tcgcctgggc ccgcagtgac
     9181 ggcgggacgc ccgcgagccc ttccacggcc tggcagtagg ccagggcgtg accgatcgag
     9241 gtgtcgccgg ccagcgtttc catgtagtgg attgtgcgtt tgtttggccc gccgacgagc
     9301 gcccgctcca cgccgcggtg ctgataaccg aggtgaatct ccaggtgaaa cacgtgctcg
     9361 ccgtggcact gaaagcggaa gctgcccggc tcgatgacgc cagcgtggac cgggcccacc
     9421 gcgacctcgt gaatctcgct cccggcgacc tggaaatgct ccgtcacgca gggcaggatc
     9481 tgctcgccca ccgcgcggcc ccaggcgtcg tggccgggcc ggtacgagtg gtgaaagcgg
     9541 agcggcttca gccagggatg cccgcgggga accagacccc attgctcggc gatttcgcgc
     9601 tcgaaattgt gggcctgcgg gcaatcgggc gtcagcgaag ggaactcgtc gctgtcccga
     9661 tcgcacgtca gcggcgaaag cagcccctca gtctcgtcgg ccagcacggc atacaggcgg
     9721 acgccgttgc cctcccgcgg ctggccgaac agagccacca ggcgcgttgt gccgttggcg
     9781 cgcgagacga tcgtttcgcg gaaccgggtg aagtcgagat gcggcacctt cgccaaggaa
     9841 atagcctgac cgtttgtcaa gcgcggaagg cggtgggcgg cggacggcat tgggaaacct
     9901 catcccggtg agggcgcgcc ttcaagaaac attgccgcgt cgcgcaacat cgttgccagt
     9961 ggcggcggca gatacacgcc gagcaccagc accagcacgg cgaaggcgac gatgggccag
    10021 caggtcgcag cgctgtcgtg aatcgcggtg ctcgtggcgg cagggctcgg cgcgccaaag
    10081 accatcgatg tgaccgttgc gccgaacccg ataaagacca ccagcagcat gatcgcgaac
    10141 agcgctccct caacaaaccg cccctgccga aatgcggccg ccaggagcga aaactcgctg
    10201 acaaacggtg caaatggcgg cgagccggtg atcgccagga atccgaggag gaacagccaa
    10261 ccagaaaccg gcagccggcg gatcacgccg cgcacatcgg cagtgagctt ggtctcatac
    10321 gcccggtgga tattgccggc cgagaggaac aagatgccct tggtcaagcc gttgttgatc
    10381 gcgtgcaaca gcgcggccca cgttgcggcg ccgccgatgc cgatgcccag tacgagaatt
    10441 cccatgtgct cgacgctgga ataggcgagc atgcgtttga agtcgcgctg gcggaccatg
    10501 aacgccgccc ccacgccgat cgagagcagg ccgacgaaga ccatcatgcg atgggcaaac
    10561 tgggcgtcgt gcgcggcgtt ggtgatgtgg acgaatctca ggatgcagag aaacgcacag
    10621 ttggtaatgc caccggccaa gagtgcgccg atcatgcctg gggcctcgcc gtaggcatcg
    10681 ggcttccagg tatgcattgg cgccaagccc atttttgtgc cgtaaccgac gaacaggaac
    10741 acgaacgcag cgtgcatcca ggggcgcgac aacaacggcg cctggcgaac cagatcgtcg
    10801 aagagcaggc tcgttttcag cccggagtgc agcgccgcgt aggccaggaa cagcgagccc
    10861 atcaaggcca gggcgatgcc cacggaacag atcagcagaa acttccacgt ggcttcgagc
    10921 gaacgcgggt tatgattgaa atacagcccc ggcgcgctga tgagcgtggt cgcttccatc
    10981 gcaacccaca tcaggcccaa gtggtgggac aggatgatca gcgtcatcat cgacagcgac
    11041 agcagcaggc aggcgcagag cacgcggttg tcccgctggc tgcgctgggc gaggtagcct
    11101 ggcgcgtagc aggcgcacaa gaagaagagc acgctgatca gtcccaggaa gattctgccg
    11161 agcgggtcga gattgagcca gttgtcccag ccggtcagat ttgtctcctg gagggcgcgg
    11221 gcagcgagca cgagctggac gagtcctcca gccggcagca cccagggccg ccagcggttc
    11281 gacggcacgg caaaggcgag ggccgcccag gcgacgggta ggcagaccag cagttcggcc
    11341 atgcgtcatt ccttgcgggt cactctttga gggccgacag atggtcgctc gtcgaagcgg
    11401 tgaactgccg attcacgtga tggatgatga ttcccatcac gaagacgccg acgaacaggt
    11461 cgagcagcac gccgacctcg acgatcagcg gcagcgcatc gaccagcagc aagccgaaga
    11521 tgaaaatccc gttctccaac accaggtatc ccagcacctg cgtcagggcc tcgcggcggg
    11581 tggtgagtat caagaaggcc gtgaatacgg tcgagagcga tgccgggacg aggaggtggc
    11641 tggcgtgttc cgaccgcagg gggagcgtcc gcgacagggc cagggccagc cccgtgccga
    11701 tcgcgccgag caacagcgag gtcgtcaatc cgaggaaggg ttcgatgcgc cacgagacgc
    11761 gcgcctcgcg catggcatag gtgagcatcc gcggcatgac gagccctttg atagcaatca
    11821 tcgccgccgc caatgccagg aggcggaggc tgacgacgct ggttcctgcg gcgtggagcc
    11881 cttgatgagt cagcggataa atgatcccca aaatggcccc ttgcaaggcg accgcaaaga
    11941 tcagggcctt gagccggccc gtcccgagca gaaaaaaatt cagcagcacg acggctacca
    12001 gaaacgggtc gatggtggtg aggttcatag cgcgaatcgg ctcaaggtaa agtgaccagc
    12061 agcacgaaac caaaagccgc gattacggcg gcggcagtca gcacgtgcgg caccagcgtc
    12121 atccgcaggc gggccatgac cgattccacg atgccgagga ggacggccac aacgcagaca
    12181 ctagcggcga acgccgggta actgacccag gcgttctgag ggtccagcgg cacggccagc
    12241 cgcacgagca gcgaggcaaa cacaaacagc ttgatcgcgg cggcctcttg caagagggcc
    12301 agcatcggcc cgctgtgatc gaggacgatc acttcgtgga tcatggtcaa ttccaggtgc
    12361 gtgttgggat cgtcgaaggg aatacggcag ttttcggcca gcatgagcag gaaccagctc
    12421 atcagcacga gcagcagggg cgcggcggcc gcgaaggtgc cggaaaacag agacccgcga
    12481 aatagctcgg ccatctgcag cgagccggta agcttggcca ggacgagcag gcagaagaac
    12541 atcgccggct ccgcgaaaca ggcaaacgtc acttcgcggg cagcgcccat cccctcaaac
    12601 gctgagcccg tgtcgagtgc cgacagcacg aggaaaaacc gtcccaaagc gagcaggtag
    12661 gcgaacaaca gcagatcacc ggcaaaccca agcggcgctg tgttgtgtcc cagcggtatg
    12721 aatagcgccg ccagcagcgc ggtcaccagg ctcaccacgg gaccgaagcg aaagatccac
    12781 gtcgtcgtgg tgctctgcac ggaacccttc cgcagcagac gccagaggtc gtagtacggt
    12841 tgcaggagag gcggtcccgc gcgaccgccg aagaaggcct tggtgcgatt gatgatgccg
    12901 agcagcaacg gcggcagcgt gcccactaac agcaggtgca gcacgttgtg aaggatggtt
    12961 agcacggctt gcggcctagc gaaagacgag cagcagcaat acgaggatgg caaagacata
    13021 caggagataa atctggacgc tgccttgctg cattaggcgg aaccagaaca gaattcgcgc
    13081 cgccaaatga aatgccggca tgaccagccg atccagcacg acttccggaa cgtggctctc
    13141 aaaatgggcc gattgcggaa aaagactggt cagtcgcggc tgatgcgcgg tcggctgtag
    13201 tgcccagccg aacagcgcga cgagaaactg ggcgaaactc gaagccgtgt actgcattcg
    13261 gggcgtcgga cgggtgtagc cacagcccca ggtttctgtc cagccgagcg gctgagtccg
    13321 cattcgccag ttgaggaccg ccgtgccgat ggccagtgcc gcgatgaggg caacggccaa
    13381 aacgctgact tgcggtagcg ccgctgcctg gaccaagggc tgcgcctccg ccagagactg
    13441 gccgccccaa atcgcagtga ctttgtcgag cagcggcgcg accagcgtgg acccgagtcc
    13501 gattgcaaag caacacccgg ccagtgcgaa ttggggcaag agcatcgtcc acggcgactc
    13561 gcacgccgcc tgtggatgcg gctcgcgacc gacgcccaga aagaccacgc caaagacctt
    13621 cacgaagcag gcgaccgcca gcgcgcccat aagagccagc gccggcacgg caaaggccac
    13681 cacgggaaat cccggctcct gtttcagccc cagcgtgtgg agcaggcccg tgtagatcac
    13741 gaactcgctt acgaaaccat tgagcggtgg caggccgcag atggctgttg ccc
//
LOCUS       DASUGB010000874         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_791757, whole genome shotgun sequence.
ACCESSION   DASUGB010000874 DASUGB010000000
VERSION     DASUGB010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791757"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV87_16665"
     CDS             <1..204
                     /locus_tag="VFV87_16665"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909338.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5105454.1"
                     /translation="VATAQQRGCRLADLTPHDFQTAHVHLTDEVYNILGVEKAVAAFK
                     SYGSTAPAEVRKQIDQWKARLAS"
     gene            351..2681
                     /locus_tag="VFV87_16670"
     CDS             351..2681
                     /locus_tag="VFV87_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105455.1"
                     /translation="MALCAASASAQDPFAAPPAGGRPMPMGGEVDPFAPGAARLGEAP
                     PPVDTSDPTKREPLVIQQLRDSNPTRPAELMQVAQSAFQFGRPDETRRYLAKLLASMP
                     AEDALAPLPGQFGDFLLLLTREEQVQPEGKQAADLVLAAAQKQAQDPDRIDALIAQLS
                     NDDLAVRQEALYELSKSGTHVVTPALRVLADASREKEHRFIRVALAHLKTSTELPLLG
                     ALDVPNDYLKAQVILILGRMGSGKALMHLVRYAVDPGVPAEVRDSALAALTKISGPIP
                     DRYEAERYLAQQTARLLQGQIPYRADHENLVELWTWDESKREVVPRKLPIGDASVLLS
                     TRTANDLYALQASGAGESASGGRQPPETAADADAYNRALRLKLLTNLELAKVLAGLGQ
                     PLPMGEGTAGGLAAAAGPQIVSQVLDDALENDRIPAAIAAAEVLGQLGDPFILLTKDG
                     RASPLAGALIHTDRRVRLAAALAAIKLDPGESFPGAGRVAPALGWIAGTGGESLVLVG
                     HPRGEDAQTLVGFANELGMGGQAAYIGRTLAEEAMANPDYELILISDAIDLPPVKELV
                     QWLRRDYRTAQIPVGVMIRGETLDAARFAFRDDPYTTVFPRIHTTEAAAFELHRLQEL
                     AGRNLVGRDERIDQARTALAALAQLASKPSNFARFELLRQEPAVIQALNHPALTADAA
                     AVLAAFATPKGQTALVDFASQHTRDLAQRQAAAAAFAAAVKNRGLLLTQTQIAQQYAR
                     YNASERLDKPTQDLLGSILDAIEAPAIGRGDLTRAE"
     gene            3113..>3858
                     /locus_tag="VFV87_16675"
     CDS             3113..>3858
                     /locus_tag="VFV87_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5105456.1"
                     /translation="MEAVYRLTRKVARTGASVLLLGETGTGKEMIATAIHRLSHRASG
                     PFVRVNCGALSESLLESELFGHVRGAFTGAVNNRTGRFEAAHTGTIFLDEINSTSLYL
                     QVKLLRVLQEREFERVGDTTTNRVDVRVIAASNRDLYGQVEAESFREDLYWRLNVVPI
                     IIPPLRMRREDIPALVAFFLNQYSEKNDKNVVHIQPEAMTALQDYHWPGNVRELQNYV
                     ERAVAMAEGDELTMELLPDAVLGRAERRAGR"
BASE COUNT          682 a         1353 c         1185 g          638 t
ORIGIN      
        1 gtcgccaccg ctcaacagcg cggctgccgg ctggcggacc tgacgccgca cgacttccag
       61 accgcccacg tccacctgac tgacgaggtc tataacatcc tgggcgtcga aaaagccgtc
      121 gccgcgttta agagctacgg ctcgaccgcc ccggcagaag tccgcaagca gattgaccag
      181 tggaaagcgc gactcgcatc atgaccaccc acgccattca cctcgttccc aagctccgct
      241 tgggaccgca cgtaccgaag ctccgcttcg agagcagccc aggaagcaga gcttccagac
      301 ttgcgttccc aagcggagct tgggaacgag gactctctgc gctgtttgcc gtggcgctct
      361 gcgccgcatc cgcctccgcc caagacccct tcgcggctcc gcccgctggc gggcggccga
      421 tgccgatggg gggcgaggtt gatccgttcg ctccgggcgc cgcgcggctg ggcgaagcgc
      481 ccccgccggt ggacacgtcc gatcccacga agcgcgagcc gctggtcatc cagcagcttc
      541 gcgacagcaa tccgacgagg cccgccgaat tgatgcaggt ggctcaatca gcctttcaat
      601 ttgggcggcc ggacgagact cgccgctacc tggcgaagtt gctggcctcg atgccggccg
      661 aggatgctct cgcgccgctt cccggacagt tcggcgattt tcttcttctg ctcacccgcg
      721 aagagcaggt ccagcccgag ggcaagcaag ccgccgatct ggtgctggct gcggctcaga
      781 agcaggccca agacccggac cgcatcgacg cgctcatcgc ccagctttcc aacgacgacc
      841 tcgcggtccg gcaggaggcc ttgtacgagc tgtccaaatc cggcacgcac gtcgtcacgc
      901 cggcgctccg cgttttggcc gacgccagcc gcgagaagga acaccgcttt attcgcgtgg
      961 ctctcgccca tctgaaaacc tctaccgagt tgccgctctt aggggcgctc gacgtgccga
     1021 atgactatct caaggcgcag gtcatcttga ttctggggcg catggggagc ggcaaagcat
     1081 tgatgcacct ggtgcggtac gcggtcgatc cgggcgtgcc cgcggaagtg cgcgattccg
     1141 ccctggccgc gctgaccaag atctccggtc ccatccccga ccgctacgag gccgaacgct
     1201 atctcgccca gcagacggcc cgcctcttgc aagggcagat tccgtaccgg gccgatcatg
     1261 agaatctggt ggagttgtgg acctgggacg aatccaagcg cgaggtagtt ccccgcaagc
     1321 tgccgatcgg cgacgccagc gtcctcctct ccacccgcac ggcaaacgac ctttatgccc
     1381 tgcaggcgag cggggcgggc gaatcggcga gcggggggcg tcagccccct gagactgccg
     1441 cagacgccga cgcctacaac cgcgccctgc gcctcaagct cctcaccaac ctggaactgg
     1501 ccaaagtcct cgccggcctg ggccagccgc tgccgatggg cgaagggacc gccggcggat
     1561 tggccgccgc ggccggtccg cagatcgttt cgcaggtatt agacgacgcc ctggaaaacg
     1621 atcgcattcc tgccgccatt gctgccgcgg aagtccttgg gcaattgggc gatccgttca
     1681 ttctcttgac caaggacggc cgcgccagcc cgctggctgg cgccctgatc cacaccgatc
     1741 gccgcgtccg cctggcggcc gcgctggcgg cgatcaagct cgatccgggc gaatcgttcc
     1801 ccggcgccgg ccgcgtcgct cccgcgctgg gctggatcgc cggcactggc ggcgaatcgc
     1861 tcgtcctggt cggccatccg cgcggcgaag atgctcagac gcttgtcggc tttgcgaacg
     1921 agctgggcat gggcgggcag gcggcctaca tcggccgtac tctcgccgaa gaagccatgg
     1981 cgaatcccga ttacgagctg attctgatca gcgacgcgat cgacctgccg ccggtgaaag
     2041 agctcgtgca atggctgcgg cgcgactacc gcacggccca gattcccgtc ggcgtgatga
     2101 ttcgcggcga aacgctcgac gcggcccgtt tcgcgttccg cgacgatccc tacaccaccg
     2161 tctttccgcg catccacacg accgaggcgg cggctttcga gctgcatcgg ctgcaggaac
     2221 tggcggggcg gaacctggtg gggcgagacg agcgcatcga tcaggctcgc acggcactgg
     2281 cggcactggc tcagttggcc tccaagccgt cgaatttcgc ccgctttgag ctgctccgcc
     2341 aggagccggc ggtcatccag gccctgaatc accccgccct gacagcggat gcagccgccg
     2401 ttctggcggc tttcgccacg cccaagggcc agaccgcctt ggtcgatttc gccagccagc
     2461 acacccgcga cctggcccag cgccaggccg cagccgcggc tttcgccgcc gccgtgaaaa
     2521 accggggcct gttgctcacc cagacccaaa tcgcccagca gtacgcacgc tataatgcca
     2581 gcgagcggct cgacaagcca acgcaggacc tcctcggctc gattctggac gcgatcgaag
     2641 cacccgcgat tggccgcggc gatttaacgc gggcagaatg acgaaatcca aggaccaagc
     2701 cccgaagtca aaatgaccga aactcggtac gatgcaggtg gtgcgaaagt tttgagcatt
     2761 tgtgccttca gtagtcccac tcgtggctgt gaattgcttc ttcgacttcg ccgaccaaca
     2821 gaatatccac ctcgccctcg cgcccctgca tgataagtaa ccctactgcc taacattccg
     2881 gcaactgctg agcccaggcg tttgacgcgt gggcttgcgt cgtttaatcg catccccatt
     2941 cccgtcgtag ccgcactcgc cagagtgcgg gaaacaggat caccagcgtt gctgcccgcg
     3001 ttctggcgaa cgcggctaca cgcgaacgga ctctaccatg tcgatctatc gctctcatcc
     3061 cgccgctgaa accgggccgc cgatccccgg cctcatcggc gccagccccg ccatggaggc
     3121 tgtctatcgg ctgacgcgga aagtggcccg gacgggcgct tccgtcctct tgctgggcga
     3181 gaccggcacg ggcaaggaga tgatcgctac ggccatccac cgcttgtcgc accgcgccag
     3241 cggcccgttc gtgcgtgtca actgcggcgc gctgagcgag agtctgctcg aaagcgagct
     3301 ctttggccac gtccgcggcg cgttcaccgg cgcggtcaac aatcgcaccg gccgattcga
     3361 ggcggcccac accggcacga tcttcctcga cgagatcaac agcacgtcgc tctatctgca
     3421 agtcaagctg ctgcgcgtcc tgcaggagcg cgagttcgag cgcgtcggcg ataccacgac
     3481 caaccgcgtc gatgtccgcg tcatcgccgc cagcaatcgc gatctctacg gccaggtcga
     3541 agccgaatcg ttccgcgaag acctctactg gcggctgaac gtcgtgccga tcatcattcc
     3601 gcccttgcgg atgcggcgcg aggacatccc ggctctcgtc gccttcttcc tcaatcaata
     3661 cagcgagaag aacgacaaaa acgtcgtcca cattcagccc gaggcgatga cagccctgca
     3721 ggattatcac tggccgggca acgtccgcga gctgcagaac tatgtcgagc gggccgtggc
     3781 gatggccgag ggcgacgagc tgacgatgga gctactgcct gacgccgtgc tggggcgggc
     3841 ggagcgccgc gcaggccg
//
LOCUS       DASUGB010000875         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1069682, whole genome shotgun sequence.
ACCESSION   DASUGB010000875 DASUGB010000000
VERSION     DASUGB010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069682"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..574
                     /locus_tag="VFV87_16680"
     CDS             191..574
                     /locus_tag="VFV87_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105457.1"
                     /translation="MRFRLRTLLIALAVLPPILAVVGVALFDLKQEPPSVVGRVTFRG
                     MPADAVAVVFSPPDAPARSYAAKTDAAGMFRVTADSKGRPLRAGSYRVSISGQALPAR
                     YQNPTTSGLAVEVSGPETTLDFELQ"
     gene            594..1172
                     /locus_tag="VFV87_16685"
     CDS             594..1172
                     /locus_tag="VFV87_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105458.1"
                     /translation="MSKYHNDNSHASDPGQFALAPHRFRRLEPAARGLTGWLWRQFWD
                     GRSFEELLDEHLNLGDTRAAMVVATQPLVVAAYTDELDCVALLGFPEWLVGEFALRPG
                     TRLLTVNTYETGDQVMSDLVPGPEDTQNYVNFFPVIADFFSDDQDRIVPRKAQIDERE
                     WRRCQAMASEALERCQGRWRNGSPLYSFRPAT"
     gene            complement(1289..>3020)
                     /locus_tag="VFV87_16690"
     CDS             complement(1289..>3020)
                     /locus_tag="VFV87_16690"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5105459.1"
                     /translation="SERRALQEQLATLQTKLQKDQEAIDKLTNVAPAIPAPDPAIAPP
                     TAAANPAAVPSAESQAAPRPAAESSPPPATAVEAVPQAMSVLPANPLTSPIEPAGESI
                     TPIVPGAAGTANAALTAQKAPTKELLEATEKARVKEEAAQAAEEAARSITERIELLRA
                     NVKIGRQLRDTTRQKVDNADQTVRILEGQLEAKISGGAPPFEIADLKQRLAEASGRLR
                     QFRAESRERVDYLDAQQSELAALQGEQITSLRLAEARRQEADQAQAFVEQLQNPLSPQ
                     NMLAWGTDHAPRLALILLATIACLWFSRRLEGRIVKFVASRGHRGSAEDSENRVRTLV
                     GVVHNAASLTVITAGVLLISDELGVPVGPLMGGAAVVGLAIAFGAQSLIKDFFTGFMI
                     LMEQQYMVNDVVKIGGISGQVERITLRTTVLRDLEGCVHFIPHGTITSTSNLTHGWSR
                     ALFEIGVAYKENVDQVMDLLLDIGRELRRDREFGPLILDDPVMLGVDSFGDWSVNIKF
                     YIKTRPLRQWPVKREMLRRIKRAFDRAGIEIPFPHRTLFLHSGHAGDGIHSPENMGLA
                     PPGFRDSEAA"
BASE COUNT          480 a          958 c         1009 g          573 t
ORIGIN      
        1 ggcagatatt tgccatcttg aaacgggtca accgtgggca gccgcggtgg caacaagtca
       61 gacactattt ccgcaagcgt ttcgctttga aatcgcccag cgggtcctcg gcgttgattt
      121 tgctccgata ggtggactca aattcgattg aacaggtcgt tcggccactc cgcccggcta
      181 caatcaggcc atgcgcttcc gcctccgcac gctactcatc gcgctggccg tgctgccgcc
      241 aattctggcg gtagtgggag tcgcgctgtt tgacctgaag caagagccgc cgtcggttgt
      301 gggccgcgtg acctttcgcg ggatgccagc cgatgcggtc gctgttgtct tttctccgcc
      361 ggatgctcct gcacggagct acgctgccaa gacggacgca gcgggcatgt tccgcgtaac
      421 cgccgattcc aaaggccgcc cgctccgagc gggcagctac cgggtcagca tttcaggcca
      481 ggccttgccg gccagatatc agaatccaac gacatcggga ctcgccgtcg aggtctccgg
      541 gccggaaaca acgctggact tcgaattgca atagctcgcc cgagtgctgc gcgatgtcca
      601 agtatcacaa cgacaacagc catgcctccg acccgggcca gttcgcgctg gcgccgcacc
      661 gctttcggcg cctggagccg gcggctcggg gattaaccgg ctggctgtgg cgacagttct
      721 gggatggccg cagcttcgag gaattgctcg acgagcatct gaacctgggc gatacgcggg
      781 cggcgatggt cgtcgccacg caaccgctgg tcgtggccgc ctataccgac gaactggatt
      841 gcgtggccct gctcgggttt ccggagtggc tggtcggcga gttcgccctg cgtccaggca
      901 cgcggctgct cacggtgaat acctatgaga ccggcgatca ggtgatgtct gacctcgtgc
      961 cagggccgga agacacgcag aactacgtca acttctttcc ggtgatcgca gatttcttct
     1021 ccgacgacca ggaccgcatc gtcccgcgga aggcccagat cgacgagcgc gagtggcggc
     1081 gctgccaggc aatggcgagc gaggccctgg aacgctgcca aggccgctgg cgcaacggct
     1141 caccgctata tagcttccga ccggcgacgt aacgggaccg tgaagctgct gcagctccgt
     1201 agccgcgctc gccagagtgc gggcgaaaag gccggtgttc ccgcgttctg gcgaacgcgg
     1261 ctacgatgcg cgttgttgat tccgccgctc acgccgcttc cgaatcgcgg aatcccggcg
     1321 gggccagccc catgttttct ggcgagtgga tgccgtcccc ggcgtggccg ctgtgcagga
     1381 agagcgtgcg gtgcgggaag gggatttcga tcccggcgcg gtcgaaggcg cgcttgatcc
     1441 gccgcagcat ctcgcgcttc acgggccatt gccgcagcgg acgcgtcttg atgtagaact
     1501 tgatgttcac gctccagtcg ccgaaggaat cgacccccag catcaccgga tcatccagga
     1561 tcagcgggcc gaactcgcgg tcgcgccgca gctcgcggcc gatgtccagc aagaggtcca
     1621 tcacctgatc gacgttttcc ttgtacgcca cgccgatctc gaacagtgcc cgcgaccagc
     1681 cgtgcgtcag gttcgaggtc gaagtgatcg tgccgtgggg gatgaaatgg acgcagcctt
     1741 ccagatcgcg gaggacggtg gtccgcagcg taatccgctc gacctgtccg ctgatgccgc
     1801 cgattttgac cacgtcgttc accatatatt gctgctccat gaggatcatg aagccggtga
     1861 agaagtcctt gatcaggctc tgggcgccga aggcgatcgc caggccaacg accgccgcac
     1921 cgcccatcag cgggccgacc ggcacgccga gctcatcgga gatcaagagc acgccggcgg
     1981 tgatgaccgt caggctggcc gcgttgtgga cgacgccgac gagcgtccgc acgcggtttt
     2041 cgctgtcctc cgccgagccg cgatggccgc ggctggccac gaatttcacg atccgccctt
     2101 ccagccgtcg cgagaaccag aggcaggcga tcgtggccaa gaggatcagc gccaggcgcg
     2161 gggcatggtc ggttccccag gcgagcatat tttgcggcga gagcggattc tgaagctgct
     2221 cgacgaaagc ctgggcctgg tcggcctcct ggcggcgggc ctcggccagt cggagcgacg
     2281 tgatctgctc gccctggagg gcggccagtt cgctttgctg ggcatcgagg taatccacgc
     2341 gctcgcgcga ttcggcgcgg aattgccgca agcggccgct ggcttccgcc agccgctgtt
     2401 tgaggtcggc gatttcgaac ggcggggcgc cgccggagat cttggcttcg agttggcctt
     2461 cgagtatgcg gaccgtttgg tcggcgttat cgaccttctg ccgcgtggtg tcgcgcaatt
     2521 gccggccaat cttcacgttg gcccgcaaca gttcgatacg ctcagtgatc gaacgggccg
     2581 cctcctcggc agcttgggcg gcttcttcct tgacgcgggc tttctcggtc gcttcgagca
     2641 gctctttggt gggcgctttt tgggcggtca gcgcggcgtt ggccgtgccg gcggctcccg
     2701 ggacgatagg cgtgattgac tcgccggccg gttcaatggg gctcgtgagc ggattggcgg
     2761 gcagcaccga catcgcctgc ggcaccgctt ctacagcggt agccggcgga ggactgcttt
     2821 cagcggcggg ccgtggagcc gcttgcgact cggccgatgg gacggctgcc ggattcgccg
     2881 ctgcagttgg cggtgcaatg gccggatccg gggcggggat cgccggagcg acgttcgtca
     2941 gcttgtcgat cgcttcctgg tccttttgca gcttggtttg gagcgtggca agctgctcct
     3001 gcagcgccct ccgctcgcta
//
LOCUS       DASUGB010000876         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1208832, whole genome shotgun sequence.
ACCESSION   DASUGB010000876 DASUGB010000000
VERSION     DASUGB010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208832"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2201
                     /locus_tag="VFV87_16695"
     CDS             105..2201
                     /locus_tag="VFV87_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105460.1"
                     /translation="MLASLAVWAVMLNLGGDSAPLPEARSPASPTQPTPPAPNAAKPI
                     SPQPQQNVAATQDSGPSAAPDRRPFPVQALKPVTPEPAEPEPKRYTWTEIEKLMDDLR
                     EQRKTNPRAVHELHDWYQHLSDPKFTQSVDYPQHFERLATWRTEVPDSSTRLVVLARA
                     YIDYAWRARGSGFAYTVTDQGWELFHVRVSEARRLLDEAVKLGVKDGEAYSAYITVAK
                     AEGFDTAQARLWLKEGQRVDPTYYEMYASMAEYLLPRWHGGPGDVERFAAEVAAELPG
                     DDGAEAFGQIAFRTNLYENPYSPTLFWGGYDRRLLARSADVIASRYPDSRKFVNFAAL
                     CAYVAQDREVARRIRPLIGEFDQNDGIWLWKNSHDGFLHWCEGTEPGGEECWLWAPPS
                     GTMGMAFHPDGKRLWIAAQFGSSAVDLLDLGSGRIEGSLPSPGGTLNQVAFDDKRQWI
                     VTSFWREYQGFILWDNSTFDIVRHPTAEQVRALAIHPEKPEIAWAESKTVRFMNTTSG
                     EVLREWSLPGYVHELRFSDDGSLLAVKTNKLTVFDAASGEVKHELSPRNDMSRSEWAL
                     ESLLRIDSEGRLWATQQLRTDASRPHRLARSTPDGKSWEVVIPEIGAGIAVLSPDRRI
                     LAVRTTPLSGKEEVTPVDIWDVPSARRIKQLPGHWNDISKMVFSADGKRLATVAGMSD
                     IVKIWLLQDDAKVAAP"
     gene            complement(2242..>3216)
                     /gene="menC"
                     /locus_tag="VFV87_16700"
     CDS             complement(2242..>3216)
                     /gene="menC"
                     /locus_tag="VFV87_16700"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX5105461.1"
                     /translation="VFATAKEWLAPALVGQDIQRGDDLQARLSHFKGNPFAKAALDCA
                     WWSLESQRSGKPLHVLLGDAAGAVGNALRGVPQMRDEVPVGADFGVMDTIDELLPLVA
                     GAVQDGFPRIKLKFRPGWDLPMLRAVRKRHPDHTFHIDCNSGYKLEDTPLFREVDELN
                     LAMIEQPLAHDDLVDHAALQAQIRTPVCLDESITGLRQTEQAIRLKSCRYVNIKPGRV
                     GGLTNAIKIHNVCRAAGIPCWVGGMLESAVGASHCVALAMLDNFTYPADIFPSSRFYR
                     EDLADPPLELIRTAEGLPGVRPFAIMPTPHPQRLESLSVARAAIRATA"
BASE COUNT          639 a          966 c         1019 g          592 t
ORIGIN      
        1 tggacctcat ttcgacggcc aggagcgtct aaccccgcgc cgcgggctcc cgatctgtgg
       61 aggtatatat ggcgaatcga gtattgacgg ggatcgcgtg tgtcatgctc gcgtccttgg
      121 cggtgtgggc ggtcatgctc aacctcggcg gcgattcagc accactgcca gaggctcgtt
      181 cgccagcatc gccgacacag cctacgcctc cagcgccaaa cgccgcgaag ccaatctcac
      241 cgcagccaca gcaaaacgtc gctgcgacgc aagattccgg gccgtcggct gcaccggatc
      301 ggcggccctt ccccgtccag gccctgaagc cggtcacacc ggagcctgcc gaaccagagc
      361 cgaagcgcta tacctggacc gaaatcgaaa agctgatgga cgaccttcgc gagcagcgca
      421 agaccaatcc gcgggcggta cacgaactcc atgattggta ccagcatctc agcgatccga
      481 aattcacgca gtccgtcgat tacccgcagc actttgagcg gctagcaacg tggcgaactg
      541 aagtccccga ttcatcgact cggctcgttg tgctggcccg cgcgtacatc gactacgcct
      601 ggcgagctcg gggatcagga ttcgcctaca cagtgaccga ccagggctgg gagctgttcc
      661 atgtccgcgt ctccgaggcc aggcggctct tggacgaagc ggtcaaacta ggggtcaagg
      721 atggcgaggc atatagcgcc tatatcaccg tggccaaagc cgaggggttc gacacagctc
      781 aagcccgatt gtggctcaaa gaaggtcaga gggtcgatcc cacgtactat gagatgtacg
      841 cgtcgatggc cgaatacttg ctcccgcgct ggcacggcgg gccaggcgat gttgagcgat
      901 ttgctgccga agttgccgcg gaacttccgg gcgatgacgg agcggaagcc tttggccaga
      961 ttgcgttccg cacgaatctc tacgagaatc cgtattcgcc cacgttattc tggggcgggt
     1021 acgatcggcg cttgctggca aggtccgccg acgtgatcgc cagccgctat ccggattcgc
     1081 ggaagttcgt caactttgcg gcactctgcg cgtatgtcgc tcaggatcgg gaagttgcca
     1141 gacgcattcg cccgctcatt ggggaatttg accaaaacga cggaatctgg ctctggaaaa
     1201 actcgcacga cggctttctc cattggtgtg agggaacaga gccaggcggt gaagaatgtt
     1261 ggctctgggc gccgccatcc ggcacgatgg gaatggcatt ccacccggat ggcaaacgcc
     1321 tctggatcgc ggcgcaattc ggcagctcag ccgtggattt gctcgatctg ggcagcggcc
     1381 ggatcgaggg ctcactgccc agccccggcg gaacgctgaa tcaagtggcg ttcgacgaca
     1441 aacgacagtg gattgtcacg tccttctggc gggaatatca aggattcatc ctgtgggaca
     1501 atagcacctt cgacatcgtc cgccatccca ccgcggagca ggttcgagcc ctcgccatcc
     1561 accccgagaa acctgagatt gcctgggccg agtccaagac agttcgtttc atgaatacga
     1621 ccagcggcga agtattacga gagtggtcgc tgcccggata tgtccacgag ctgcggttct
     1681 cggacgatgg atctctcttg gccgtcaaaa cgaacaaact gaccgtattc gatgctgcgt
     1741 caggagaagt gaagcatgag ctttcaccgc gcaacgatat gtcacgatcg gaatgggcct
     1801 tggagtccct attacggatc gactctgagg gccggttatg ggccacgcag caactcagaa
     1861 ctgacgcctc caggccgcac cgcctcgcgc gttccacacc ggacggcaaa tcgtgggagg
     1921 ttgtgattcc cgagatcgga gcgggaattg cagtactttc acccgaccgt cgcattctgg
     1981 cagtcaggac cacgccactc tcgggcaagg aggaggttac acccgtagat atctgggacg
     2041 tcccaagtgc caggcgcatc aaacaattgc ctggtcactg gaatgatatc agcaaaatgg
     2101 ttttctccgc cgacggtaag cgattggcga cggtcgcggg aatgagcgac atcgtcaaga
     2161 tctggttatt gcaggatgat gccaaggttg cggcgcccta gccccagtag aacctggcgc
     2221 ctgacgaagt ggcgctcgga tttatgctgt agcgcgaatt gccgcgcgtg cgacggacag
     2281 actctccagc cgttgcggat gcggtgtcgg cataatggca aacggccgca cgccaggcag
     2341 gccttccgcc gtgcggataa gctccagcgg cgggtcggcc aggtcttcgc gatagaagcg
     2401 cgagctgggg aaaatgtcgg cgggataagt gaagttgtcc agcatggcca gcgcgacgca
     2461 gtggctggcg ccgacggcgc tttcgagcat cccgccgacc cagcagggaa ttccggcagc
     2521 gcggcagaca ttgtgaatct tgatggcgtt ggtcaggccg ccgacgcggc ccggcttgat
     2581 gttgacatag cggcagcttt tgaggcgaat cgcctgttcg gtttggcgga ggccggtgat
     2641 gctctcgtcg aggcagacgg gtgtgcggat ctgagcctgc aacgcggcgt gatcgaccag
     2701 gtcgtcgtgc gccagcggct gctcgatcat ggcgaggttc agctcatcga cctcgcgaaa
     2761 cagcggcgtg tcttccagct tgtagccgct gttgcagtcg atgtggaacg tgtggtccgg
     2821 atgccgcttg cggacggccc gcagcatcgg caggtcccag ccggggcgga atttgagctt
     2881 gatccgggga aagccgtctt ggacggcccc ggcaacgagc gggagcagct cgtcgatcgt
     2941 gtccatcacg ccgaaatcgg cgccgactgg gacttcgtcg cgcatctgcg gaacgccacg
     3001 gagggcgttc cctacagcgc ccgccgcatc gcccagcagc acgtgcagcg gcttgccgga
     3061 gcgctgcgat tccaggctcc accaggcgca gtcgagcgcc gccttggcga acggattgcc
     3121 cttgaaatgc gaaagccgcg cctgcaaatc gtcaccgcgt tggatgtcct gccccacgag
     3181 cgccggcgcg agccactcct tggcggtggc aaagac
//
LOCUS       DASUGB010000877         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_237467, whole genome shotgun sequence.
ACCESSION   DASUGB010000877 DASUGB010000000
VERSION     DASUGB010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237467"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFV87_16705"
     CDS             complement(<1..795)
                     /locus_tag="VFV87_16705"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5105462.1"
                     /translation="MPPVTKVALIGMGTVGTGVARLLIDHGDRTARHAGRTLWVEAVV
                     VQDVKKTRDYELPQGVLTDDLNKVLKNKEIKLAAMIVGGVEPARTITLKLLESGKDIV
                     TANKLLLAEHGPELFDRARELGRSIAFDACVAGGIPITTNIAQCFSANQIQSLRGILN
                     GTTNFIVSQMEEQGATYKAALQEAQRLGFAEPDPTMDVSGGDAAQKLAILAHLAFGAR
                     VHWSEIPCVGIDALDPADLKYAKELGYRIKLIAVAQLHDGGLELHVS"
     gene            1047..>2048
                     /gene="lpxD"
                     /locus_tag="VFV87_16710"
     CDS             1047..>2048
                     /gene="lpxD"
                     /locus_tag="VFV87_16710"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX5105463.1"
                     /translation="MSLALSDIARLVEGRLTGDGDLLITGAATLAVAGPGDVTLADDP
                     KYAAMLSRCGASAVIVPSSFQPTDRPTVTVANVHQAFSRIVELFYPPRKTSRPGISPA
                     ACIDASARLADDVEVHPQVFIGADVEIGAGCLIHSGVRILDGCKIGEHCTLFPNAVLY
                     ENTVVGNRVIIHAGAVLGAYGFGYSLAQGKHKLCAQLGFVEIGDDVEIGAATTIDRGT
                     YGPTVVGEGTKLDNLVQIGHNCRLGKHNLICSQVGIAGSCSTGDYVVMAGQVGIADHL
                     HMADRSVLGAKGGVMFDTEPGKRYIGLPAVEEREAFRNAALVRQLPQMKKQIHELQRQ
                     "
BASE COUNT          381 a          620 c          671 g          376 t
ORIGIN      
        1 tgagacgtgt agctccaggc cgccatcatg caattgtgcg acggcgatca gcttgatccg
       61 gtaacccagc tccttcgcat acttgagatc agccggatcg agtgcgtcga tgcccacaca
      121 ggggatttcg ctccagtgaa cgcgggcacc gaacgccagg tgggcgagga tcgccagctt
      181 ctgagccgca tcgccgccgg agacgtccat cgtcgggtct ggctccgcga agccgagccg
      241 ctgagcttct tggagggccg ctttgtaggt cgccccctgc tcctccattt gggagacgat
      301 aaagttcgtc gtgccgttca ggatgccgcg gaggctctgg atttgattgg ccgagaaaca
      361 ctgggcgata ttggtggtga tcgggatgcc gccagcgacg caggcatcga aggcaatcga
      421 gcggcccagc tcccgcgccc ggtcgaacag ctccggcccg tgctcggcga ggagcagctt
      481 attggcggtg acgatgtcct tgccgctctc gagcagcttg agcgtaatcg tgcgggctgg
      541 ctcgactcca ccaacgatca tcgcggccag cttgatctcc ttgttcttca gcaccttgtt
      601 caggtcgtcg gtcagaaccc cctggggcag ctcgtagtcg cgggtttttt tgacatcctg
      661 cacgacgacc gcttcgaccc aaagcgtccg cccggcgtgc cgggcggttc ggtcgccgtg
      721 atcaatcaac aggcgcgcca cgccggtgcc aacagtgccc atgccgatga gtgcgacttt
      781 ggtgacgggg ggcatggggg agtcggggtc aggaatcagg agccagcaaa gccgcgcaac
      841 acccagcgag gtaacggttt acgccgatgg ccggaaactc gggcgccgac aattcgagca
      901 tcatcacagt aatcgacact cgccaggctg accactggtc gcgttagccg tccgcgttcg
      961 ctcttgcccc actttctgcg ggcggagtac gatttgcagc gacgcggcag gcgctccatc
     1021 ggccgattca ggaaaggatt ccgcacatga gcctggctct ctctgatatt gcgcgtttgg
     1081 tggaagggcg gctgaccgga gacggggacc tgctgatcac cggcgcggcg acgctggcgg
     1141 tggccgggcc gggcgacgtc acgctggcgg acgatcccaa gtatgcggcc atgttatctc
     1201 gctgcggggc gtcagcggta attgtgccgt caagcttcca gccgaccgat cggccgacgg
     1261 taacagtggc caacgtccac caggcatttt cgaggattgt cgaacttttc tatccgccgc
     1321 gaaagacaag ccgtccggga atcagtcccg ctgcttgtat cgacgccagc gccagacttg
     1381 ctgacgacgt agaagtgcat ccgcaggtgt tcatcggtgc agacgtggag atcggcgccg
     1441 gctgcttgat tcacagcggc gtgcggattc tcgatgggtg caaaatcggc gaacactgca
     1501 cactctttcc caacgccgtg ctctacgaaa atacggtggt gggtaaccgt gtgatcatcc
     1561 acgccggcgc agttctcggg gcgtatggct ttggttattc gctcgcgcag ggcaagcaca
     1621 aactctgcgc gcagctcggc ttcgtggaga tcggcgatga cgtcgagatc ggtgcggcga
     1681 cgacgatcga ccgcggaact tacggcccga cggtcgtcgg cgaaggcacg aagctcgaca
     1741 acctcgtgca gatcggccac aactgccgcc tcggcaagca caacctgatc tgttcgcagg
     1801 taggcatcgc gggaagctgc tcgacgggcg actacgtggt gatggccggc caggtgggaa
     1861 ttgccgacca cctgcacatg gccgaccgga gcgtgctcgg ggccaagggg ggcgtgatgt
     1921 tcgacaccga gccaggaaag cgatacatcg gcctgccggc ggtcgaggag cgggaggcct
     1981 tccgcaacgc ggccctagtg cggcagcttc cgcagatgaa aaaacaaatc cacgagctgc
     2041 agcggcag
//
LOCUS       DASUGB010000878         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_515447, whole genome shotgun sequence.
ACCESSION   DASUGB010000878 DASUGB010000000
VERSION     DASUGB010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515447"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="speA"
                     /locus_tag="VFV87_16715"
     CDS             <1..964
                     /gene="speA"
                     /locus_tag="VFV87_16715"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX5105464.1"
                     /translation="HHLGTVCDEAGVPHPTIISESGRAVAAYHSALVFSVLGVASQGN
                     GDESLLDIPPDREQPLHDLVDTHKNVTARNVLESFHDAQQAMDVTMTLFSTGYLTLDQ
                     RALAEDLFWAICKKIKRLTEQLDFVPEELEGLDTLLSDTYFCNFSLFQSMPDSWAIKQ
                     LFPLMPIHRLAERPNRHAVLGDITCDSDGKIDQFIDRRDVKRTLRLHAFNDQPYYLAT
                     FLIGAYQEILGDLHNLFGDTNTVHIDLTPAGEVVIEAMVKGDSVREVLDYVEFEPSDL
                     ITRLQTAVETSVREGRLSHQEAGRFLKFYEEALNGYTYLEEPGD"
     gene            complement(993..2219)
                     /locus_tag="VFV87_16720"
     CDS             complement(993..2219)
                     /locus_tag="VFV87_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5105465.1"
                     /translation="MPHLAPDDDSAWARFKSLWPLREGTTYLNHGSFGPPPEPVRAAR
                     RKWMEALESQPMDFFVRQLEPAWRAARDKLAAFVGASAGDLIFVENATVGMNIVADSF
                     PLTGGDEVLLTDHEYGAVQRIWQRACAKAGAAAKIVELPLPFRTAGETCDAIFAAAND
                     RTRLVVVSHITSPTAVILPVAEICRRARERGIALAIDGPHAVAQLPLAIDPLGCDFYT
                     ASCHKWLSAPFGSDFLRVAPRWQSLVRPPVLSWGRLPPTKIDSWSDEFVWSGTRDPSA
                     YLSIPAAIDFLETVGLDAFRQRTHWLAQYARRRLVELTGLEPIMPDDPAWYGSMAHVP
                     LPPRSHPGSARPSDDTSSVSNPLQHTIWQCYDIEVPVIDFRGHRYIRVSCHLYNDTQQ
                     IDRLVAAMHELLQQGN"
BASE COUNT          453 a          794 c          795 g          390 t
ORIGIN      
        1 tcaccacctg ggcacggtct gcgacgaagc cggcgtgccg caccccacga tcatcagcga
       61 aagcggccgg gcggtcgctg cgtatcacag tgccctcgtc ttcagcgtgc tcggcgtcgc
      121 cagccagggg aacggcgacg aaagcctgct cgacatcccc cccgaccgcg agcagccgct
      181 gcacgacctg gtcgatacgc acaagaatgt cacggcccgc aacgtcctag agagctttca
      241 cgatgctcag caggcgatgg acgtgaccat gacgctgttc agcaccggct acctgacgct
      301 cgatcagcgg gccctcgcgg aagacctgtt ctgggcgatc tgcaaaaaga tcaagcggct
      361 gacggagcag ctcgacttcg tgccggagga actggaaggg ctcgatacgc tcctttcgga
      421 cacgtatttc tgcaatttct cgctcttcca atcgatgccc gacagttggg ccatcaagca
      481 gctctttccg ctgatgccga tccatcggct ggcggagcgg ccgaaccggc atgcggtgct
      541 gggcgacatc acctgcgaca gcgacggcaa aatcgaccag ttcatcgacc gccgcgacgt
      601 gaagcggacc ctgcggctgc acgctttcaa cgaccagccg tattacctgg cgacgttttt
      661 aatcggcgcg taccaggaga tcctgggcga cctgcacaac ctgttcggcg acacgaacac
      721 ggtacacatc gacctgacgc ccgccggcga ggtggtcatc gaggcgatgg tcaaggggga
      781 cagcgtccgc gaggtgctcg actacgtcga attcgagccc agcgacctga tcacgcgctt
      841 gcagaccgcc gtcgaaacga gcgtccgcga aggccgcctc tcccaccagg aagccggccg
      901 cttcctcaag ttctacgagg aagccctcaa cggctatacg tatctcgaag aacccggcga
      961 ctgacaaagc cgcgaccagt ggtcgcggtc cctcaatttc cctgctgcag caattcgtgc
     1021 atggctgcca ccagccggtc gatctgctgg gtgtcgttat agagatggca cgagacgcgg
     1081 atgtagcggt ggccgcgaaa gtcgatgacc gggacttcga tgtcgtagca ttgccagatc
     1141 gtgtgctgca gcgggttgga gacggaggac gtgtcgtctg acgggcgcgc ggagcctggg
     1201 tgcgaacgcg gcggcaacgg cacatgtgcc atgctgccgt accacgcagg atcatccggc
     1261 atgatcggct ccagaccagt cagttcgacc agccttcgcc gggcatattg ggccagccag
     1321 tgcgtgcgct ggcggaaagc gtcgaggccg acggtttcga ggaagtcgat cgcggcgggg
     1381 atcgagaggt atgccgaggg gtcgcgcgtg ccgctccaga cgaattcgtc ggaccaggag
     1441 tcgatcttcg tcggcggcag gcggccccaa ctcagaacgg gcgggcggac gagcgattgc
     1501 cagcgcggag cgactcgcag gaagtccgag ccgaacggcg cgctgagcca cttgtggcag
     1561 ctcgcggtgt agaaatcgca gccaagcggg tcaatcgcca gcggaagctg ggcgacggcg
     1621 tgcggaccgt cgatcgccag ggcaatgccg cgctcgcggg cccggcggca gatctcggca
     1681 accggcagga tcaccgcggt cggcgaggtg atgtggctga cgacgaccag ccgcgtgcgg
     1741 tcgttcgccg cggcaaagat ggcgtcgcat gtttcgcctg cggtccgaaa cggcagcggc
     1801 agttccacaa tcttcgccgc cgcgccggct ttagcgcaag cccgctgcca gatccgctgg
     1861 acggcgccgt attcgtggtc ggtgagcagc acttcgtcgc cgccagtcag cgggaacgaa
     1921 tcggcgacga tgttcatccc gaccgtcgca ttctcgacaa agatcaaatc gcccgccgat
     1981 gccccgacga acgccgccag cttgtcccgc gccgcccgcc acgccggctc aagctgccgc
     2041 acgaagaaat ccatcggctg cgactccagc gcctccatcc acttccgccg cgcagcccgc
     2101 accggctccg gcggcggccc gaacgagccg tgattgaggt acgtcgtgcc ttcgcgcagc
     2161 ggccacagtg acttgaagcg cgcccaggcg gaatcgtcgt cgggagcgag gtgtggcatc
     2221 gaaggggtcg ggagtcgatt gcggcggcgc tgtgcattat ggccacggtc gttggcgcaa
     2281 tcgactcccg accccccagc ctcagtacca caggcgcacg cctgcgatga ggccggccac
     2341 ttgcgttggg ccgatgtcgt agtagtcgta gccgacgtag gcctcgatcg cgccaatggc
     2401 cgcaccaccg gtggctcgca ggtgatagac gt
//
LOCUS       DASUGB010000879         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1765120, whole genome shotgun sequence.
ACCESSION   DASUGB010000879 DASUGB010000000
VERSION     DASUGB010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765120"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFV87_16725"
     CDS             complement(<1..566)
                     /locus_tag="VFV87_16725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5105466.1"
                     /translation="MQSTSRRKFLAAVSAAAAVSTSGLVPFSPRVPGFLLESAAYGAE
                     KGGERILVVVQLSGGNDGLNTVVPYADEFYRKERPSLAIASGGVIKIDGSIGLHPSLS
                     GFNTLLENRQLAIVQGVGYPNPNRSHFESMDIWHSARSNAGDRKLGWLGRAFDARAAE
                     LNAAGDPPAMHLGAEVQPLALAARDVPSP"
     gene            complement(609..1976)
                     /locus_tag="VFV87_16730"
     CDS             complement(609..1976)
                     /locus_tag="VFV87_16730"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX5105467.1"
                     /translation="MANLLANLDVDAAWQPFEPTRDQPFDRRAAAHLFRRAGFAATSR
                     ELDEAVKQGPQAAVQQLLAAPAAHRAFDDEMRKFAQVTLATGNPELLTGWWLHRMRHT
                     PAPLLEKTTLFWHGHFATSAEKVRDPKLMFEQNELLRTHALGKFEELVRGIARDPAML
                     LYLDSATNRKIHPNENFAREVMELFALGVGNYSEKDIQELARCFTGWEIQYGVFKFNS
                     YQHDYGKKTVLGASGPMDGDDGLRIILDQPATPEFICSKLVRYFVTDDDELPPEFIAP
                     LAKQFRDAGLTTAPVLQTIFTSRLFYSDAAIGRKIRSPVELGVGLLRSLEATANMVQL
                     GGQLRELGQMVLYPPNVKGWAGGRAWINSSTLLGRANVVRALVENPQTQFGGGNLEAY
                     FDRLNLKSSAALVDFLDELLLAARLPVAVRTQLVASLDGEQDRPRRFNRLLHLFGSLP
                     EMQLA"
     gene            complement(1990..>3523)
                     /locus_tag="VFV87_16735"
     CDS             complement(1990..>3523)
                     /locus_tag="VFV87_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105468.1"
                     /translation="PQYEQFLAVVKRFEEQLGMPWREAARSLTNGGTYIAFDLPTQGV
                     ISLSQAADEQLAAKAQTTSLELIRQIATAQGQPDPIKEEEHRGVKIYKVGEAYSVVLG
                     RWLISSNKQVLISMLLENHFTDGETLAEDQQFRDVLKSRTGQPSAWLYVDLRVLRLTG
                     ALKQALNNKKSDNPPAEILAGGILGAIPDAAYVTASLDIVSSGIKLTASLPAKTQEIA
                     KAREFYFGPEAAGRAPPLLKPAGTLLTISTYRDFASLWRNAPDLFDEGINAKFAEAES
                     NLTTLFAGRNFRDDILGNVEPGMQIVVAKQEFPQAGITPQIKLPAGALVVRMKNPAET
                     SRMFKITFQSLIGFLNVVGGMNGLDPLEQNTEKIGDATVISSVYLPPIKEETKTEAPL
                     HFNASPTVAFVGDRFILASARPLAVALVDLVSKDEPSAGNVNTELALDGKTALAALAD
                     NRGPLIAQNMLQKGHDRAAAEKEIDTVLKAAKQIQSSSLRLTTEPDHLQLSLEVVLSD
                     TK"
BASE COUNT          620 a         1054 c         1181 g          668 t
ORIGIN      
        1 ggcgaaggca cgtcgcgggc ggccagcgcc agcggctgaa cttccgcgcc gaggtgcatg
       61 gcgggtggat cgccggcggc gttcaactcc gcggcacgag catcaaacgc ccgccccagc
      121 cagccgagct tgcggtcgcc ggcgttcgag cgggcggaat gccagatgtc catcgattcg
      181 aagtgcgagc gattcggatt cggatagccg acgccctgga cgatggcgag ctggcggttc
      241 tcaagcagcg tattgaagcc ggacaggctc ggatgcaggc cgatcgagcc gtcgatcttg
      301 atcacgccgc cgctggcgat ggcgaggcta ggacgctcct tgcggtagaa ctcgtcagcg
      361 tagggcacga ccgtgttgag gccgtcgttc ccgccgctta gttggacgac aaccagaatc
      421 cgctcgccgc ccttctcagc cccgtaggcg gccgattcga gcagaaaacc gggcacgcgc
      481 ggcgaaaagg gcaccaggcc cgaggtggat acggctgcgg cggcagagac agcggccaga
      541 aacttgcggc gggaagtgga ttgcatgatt ccctcgctta tgcgtcgggt tggtgtgctg
      601 ggataaagct atgccaactg catttcgggt aaggatccaa acaggtgcag gaggcggttg
      661 aaccggcgcg ggcggtcctg ctcgccgtcc aaggatgcga ccaattgagt gcgaacagcc
      721 acgggcaggc gggctgccag cagcagctcg tccaggaaat cgaccagcgc ggcactggat
      781 ttgaggttca ggcggtcgaa gtacgcttcg agattgccgc cgccaaactg cgtttgcggg
      841 ttctccacga gcgcgcggac gacgttggca cgacccagca gcgtcgaaga attaatccaa
      901 gcgcgtccgc ccgcccaacc cttcacattc ggcggataca ggaccatttg ccccagctcg
      961 cgaagctggc cgccaagctg aaccatgttg gccgtggcct cgagcgagcg caagaggccc
     1021 acgcccagct cgacgggcga gcgaatcttg cgaccgatcg cggcatcgga atagaacagc
     1081 cggctggtga agatcgtctg cagaacgggg gcggtcgtca ggcccgcatc gcggaattgt
     1141 ttcgccagcg gggcgatgaa ttccggcggc agttcatcgt cgtcggtgac gaagtagcgg
     1201 acgagtttgc tgcagatgaa ttcgggcgtc gcgggctggt ccaaaatgat gcgcaggccg
     1261 tcgtcgccat ccattgggcc gctcgcaccg agcaccgtct tcttgccgta gtcgtgctgg
     1321 tacgaattga acttgaagac gccgtactgg atctcccagc cggtgaagca gcgggcgagc
     1381 tcctgaatgt ctttctcgga gtagttgccg acgcccaggg cgaacagctc catcacctcg
     1441 cgagcaaagt tttcgttggg atggatcttg cgattggtcg ccgagtcgag gtagagcagc
     1501 atcgccggat cgcgggcgat gccacggacc agctcctcga atttgccgag ggcgtgcgtc
     1561 cgcagcagct cgttctgctc gaacatcagc ttgggatcgc gcaccttctc ggcactggtt
     1621 gcaaaatggc cgtgccagaa gagtgtggtt ttttccaaga gcggcgcggg agtgtgccgc
     1681 atccggtgca gccaccagcc cgtgagcagc tccgggttgc cggtggccag cgtcacttgc
     1741 gcgaacttcc gcatctcgtc gtcaaacgcc cggtgcgcgg cgggtgcggc cagaagctgc
     1801 tgcaccgcgg cctgcgggcc ttgtttcacg gcctcatcca gctcgcgact ggtggcggcg
     1861 aagccagccc ggcggaacag gtgggcagcg gcccgccggt cgaacggctg atcgcgggtc
     1921 ggctcgaagg gctgccaggc cgcgtccaca tccaagttgg caagcaggtt cgccatcttg
     1981 attcctcgct tacttcgtgt ccgagagaac gacctccagc gaaagctgca ggtgatcggg
     2041 ctccgtcgtc agccgcagcg aagacgactg gatttgcttc gccgccttga ggacagtatc
     2101 aatctctttc tccgcggcgg cgcggtcgtg ccctttttgg agcatgttct gcgcgatcag
     2161 tgggccgcga ttgtcggcca gcgcggccag agccgtcttg ccgtcgagag ccagctcggt
     2221 gttgacgttg cctgcggagg gctcgtcctt gctaactaga tcgaccagcg cgacggccag
     2281 cggccgagcg ctggcgagga tgaaccggtc gccgacgaag gccacagtgg gcgaagcgtt
     2341 gaagtggagc ggggcctcgg tcttcgtctc ttccttgatg ggcggcagat agacgctgga
     2401 gatgaccgtt gcgtcgccga tcttctccgt gttctgctcg agcggatcga ggccattcat
     2461 cccgccgacc acgttcagga agccgatgag gctttggaac gtgatcttga acatccgcga
     2521 cgtttctgcg ggattcttca tgcggacgac gagggcccca gccggcagct tgatctgtgg
     2581 cgtgatgccc gcctgcggga actcttgttt ggccacgaca atctgcatgc cgggctcgac
     2641 gttgcccaaa atgtcgtcgc ggaagttgcg gccggcaaag agcgtcgtga ggttgctctc
     2701 ggcttcggcg aatttggcgt tgatgccttc gtcgaacagg tcaggggcgt tccgccacag
     2761 cgaggcgaaa tcgcggtagg tcgaaatcgt cagcagcgtg ccggcgggct tcagcagcgg
     2821 cggtgcgcgg cctgcggctt ccggtccaaa gtagaattcg cgcgccttgg cgatttcctg
     2881 tgtcttggcc ggaagcgagg ccgtgagctt gatgcctgac gaaacgatgt cgagcgatgc
     2941 cgtaacgtac gcagcgtcgg gaattgctcc gaggattccg cctgccagaa tctcagccgg
     3001 cgggttgtcg ctcttcttat tattcagcgc ctgcttgagc gcgcccgtca ggcgcaggac
     3061 gcgaaggtcg acatacagcc aggcggaagg ctgcccggtg cggcttttga gcacgtcgcg
     3121 aaactgctgg tcctcggcca gcgtctcacc atcggtgaag tggttctcca aaagcattga
     3181 gatcagcact tgcttgttgg acgaaatcaa ccagcggccg aggacgacgg aatacgcctc
     3241 gccgaccttg taaatcttga cgccccggtg ctcttcttcc ttgatcgggt ccggctgacc
     3301 ctgagccgtg gcgatctgcc ggattagctc gagcgacgtt gtttgcgcct tggccgcgag
     3361 ttgctcgtcg gctgcttgcg aaagcgagat cactccctgc gtcggcagat cgaaggcgat
     3421 gtacgtgccg ccgttggtga gcgagcgggc cgcttctcgc cagggcatgc cgagttgttc
     3481 ctcgaagcgc ttaacgactg ccagaaactg ctcgtactgc ggc
//
LOCUS       DASUGB010000880         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1765173, whole genome shotgun sequence.
ACCESSION   DASUGB010000880 DASUGB010000000
VERSION     DASUGB010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765173"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFV87_16740"
     CDS             complement(<1..1217)
                     /locus_tag="VFV87_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105469.1"
                     /translation="MLSIYGKPRRLCTSVTRRELLTIGALGLGGLALPDLLRAEAEQG
                     LRQSKKAIIMIYLCGAPPHQDMFDLKPEAPSEVRGEFQPIPTKVPGIQVCEHLPRLAR
                     IMDKLVPIRSVYGSPNGSHDSFICYTGRSFVQQPPGGWPSVGSTVSKLLGPTHAAMPP
                     FVGLAPRAGHPPYGSPGHPGFLGSAHSAFRPNGEGQADLTLNGISAERLADRRQLLSS
                     VDRIRRSLDSKLGGQDAFTQQALGVLTSSKLAEALDVSREDPQVRERSGRGDTRNYGD
                     GAPRNQEHFLIARRLVEAGARCVTLNFGRWDFHSNNFSEFKNTHGPQFDQGMSALVED
                     LHLRGLDRDVSVVAWGEFGRTPIINKDAGRDHWPQVGCALLAGGGLKTGQIIGKTDRL
                     GGEIAERPVHFGEV"
     gene            complement(1337..2623)
                     /locus_tag="VFV87_16745"
     CDS             complement(1337..2623)
                     /locus_tag="VFV87_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5105470.1"
                     /translation="MPRRGRSTLQAEVLICGGGCAGTAAALAAARQGAKTLLVEKAPF
                     AGGIITSVGLPYFDGIASIRDHRVVVRGIALELLSKSGVCAADAKTVAKHNPTISNTF
                     EFKLLLDQLLREQKDSLTVLFNSFACGVETSGQRITGVQIANKDGLVTILAQVVVDCT
                     GDGDVAAWSGAPFEQNAEVQPLTLHFRIGRVKKSPDVSKNCRTALAAAQQRGELPYYY
                     GPGVMFLFGEDEVYVHGVRVPANPTSAADLSRAEMQGRADAHAMFRAWKRDVPGFEEA
                     YFLEAYPWIGVRESRRLVGQHVLSEDDLMQGRRFDDAIATGCWYLDLHPNKTTVGSAN
                     DFQPEKVQPEPYDIPYRSLLPRQIENLIVAGRCHSATRGAHASTRVTATAMAMGEAAG
                     TAAALAAAARHSPQELSGTKLREVLAMQGAGPFTDA"
     gene            complement(2802..3722)
                     /locus_tag="VFV87_16750"
     CDS             complement(2802..3722)
                     /locus_tag="VFV87_16750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5105471.1"
                     /translation="MTIPLSRRQVLQGAAAGSAAAAFRSVSAETSRRAPRIDTHLHCF
                     AGKDDRRFPYHARAPYGPDKPATPEHLLDCMEKAGVDFAIVVHPEPYQDDHRYLEHCL
                     SVGKGKLRGTLLLFADREGSLDKMPELCRKLDVVSVRVHAYAPDRLPPFGKPELRRLW
                     TMATDHGLAVQLHFEPRYAPGFEPLIREFSKTRVIIDHLGRPFQGTPSEHAVVVRWAR
                     FENTVMKFSAIPDKTTYPHRDIAPVIRELTDAWGAERMIYGGGFSAEATGESYRQAFD
                     RARSYLTHLSAADQDKILGGTGAKLFGFET"
     gene            complement(3755..4924)
                     /locus_tag="VFV87_16755"
     CDS             complement(3755..4924)
                     /locus_tag="VFV87_16755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5105472.1"
                     /translation="MPTANCADEKGLVASIEKVVLRRGRDGSGPTWFHPRACIVPTAD
                     GPLALMTLQTIAGSDYFGPVHWMSSRDLGQTWTDPQLVPALGRVKRQDGWEEGVCDVV
                     PEFHPATGAVLAMGHSVCYRGPRFEREQPPRWPVYAVWKDGRWGERRKLEWDDPRGSL
                     IYTNNCGQRVVLPSGDIALVMSFGAKPTSRSAAGVRCSFDGQTLTIQKVGLEMRHAAG
                     RGLLEPSLVHWRDKFYVTLRAEDSRGYVAASDDGLDFSDKQPWSWDDGEPLSMSSTQQ
                     HWLAHSEALFLVYTRKDASNINVPRWRTPLFMAQVDPDKLRLLRDTERTVLPLVGDGV
                     NAPDDVAYSGNFHPVNVSPDESWVTDGEMLPKHGYKGDLLLARIRWTRPNGLTPE"
     gene            complement(5002..>5395)
                     /locus_tag="VFV87_16760"
     CDS             complement(5002..>5395)
                     /locus_tag="VFV87_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105473.1"
                     /translation="GIDEMLVHSAEDHKVLDRLVFIGAAIESAMVREHLQAASARCHA
                     ARLAAELDQIPDAINDKTADWVYLRFLPPRETVERLHRAGKRIFLAGPLVAGNQGKNW
                     AKAVELGIDAILTDYPLELRRQLRSGNR"
BASE COUNT          987 a         1758 c         1696 g          954 t
ORIGIN      
        1 acttcgccga aatgtacggg gcgctcggca atctctcctc ccaagcgatc cgtttttccg
       61 atgatctgac cggtcttcag ccccccgccg gccagcaagg cacagccaac ctgtggccag
      121 tggtcgcggc cggcatcctt gttgatgatc ggggtgcggc caaattcgcc ccaggccacg
      181 accgaaacat cgcggtccag gccccgcagg tgcaagtcct caaccagggc gctcattcct
      241 tggtcaaatt gcggtccatg cgtgttcttg aattcggaga agttattgct gtgaaagtcc
      301 cagcgaccga agttcagcgt cacgcagcgc gcgcctgcct cgacaaggcg tcgggcaatc
      361 aggaaatgct cctgatttcg cggcgcgccg tcgccataat tacgtgtgtc cccccggccg
      421 gaccgctcgc ggacctgggg atcctctcgc gatacgtcga gtgcttccgc cagcttactc
      481 gacgtcaaca cgcccagcgc ctgctgggtg aacgcgtctt gcccgccgag tttgctgtcc
      541 aggctacgac ggatccggtc cacgctggaa agcaattgcc ggcggtccgc cagacgctcg
      601 gcgctgatcc cgttcagcgt caagtcggct tggccctcgc cattggggcg aaaagcactg
      661 tgcgccgaac ccaggaaacc gggatgccct ggggagccgt acggcggatg gcccgcacgc
      721 ggagccaggc caacgaaggg aggcattgct gcgtgcgtcg gccccagcag cttcgatacg
      781 gtcgatccga ccgacggcca tccgccggga ggctgctgaa cgaacgaccg gccggtgtaa
      841 cagatgaacg agtcgtggct gccattgggc gagccataga cactgcgaat tgggaccagc
      901 ttgtccatga tccgagccaa acgcggcaga tgttcgcaga cctgaatgcc gggaaccttg
      961 gtggggatgg gctgaaactc gccacggact tcggacggcg cctccggctt gaggtcgaac
     1021 atgtcctggt gcggcggcgc gccgcacagg tagatcatga tgatcgcctt cttcgattgc
     1081 cgcaggccct gctcagcctc ggcccgcaac agatccggca gggcaaggcc gcccaacccc
     1141 agggcgccaa tcgtcagcag ctcgcggcgc gtaacactcg tgcagagacg gcggggtttt
     1201 ccgtagatcg aaagcatgat gcaggcggga gatggggata ggcagggcag caaaggcgag
     1261 gagtgcagta tatcaacgct cgccgatgct tgctacaccg accggtactg tccccgatcg
     1321 ggatgcaata ggaagctcac gcgtcggtaa acggcccggc gccttgcatg gccagcacct
     1381 cgcgaagttt cgtaccactc agctcctgtg gactgtgcct ggccgcggcg gcgagcgccg
     1441 cggcagttcc ggcggcttca cccatcgcca tcgcggtggc ggtgacgcgg gtcgaggcat
     1501 gggcgccgcg tgtggccgaa tggcagcggc cggcgacgat caggttctcg atctgccgcg
     1561 gcagcaggga tcgataaggg atatcgtagg gctcgggctg cactttctcg ggctgaaagt
     1621 cgttcgcact cccgacggtg gtcttgttcg gatgcagatc gagataccag cagccggtgg
     1681 caatggcgtc gtcgaatcgc cggccctgca tcaaatcgtc ttcgctcagc acgtgctggc
     1741 cgaccaggcg gcgggattcg cgcacgccaa tccagggata ggcttccaga aagtacgcct
     1801 cctcaaaccc cggcacgtca cgcttccacg cccggaacat cgcatgcgcg tcggcgcggc
     1861 cctgcatctc ggcccgactg agatccgcgg cgctggtggg attggcgggg acgcggacac
     1921 cgtggacgta cacctcgtct tcgccgaaca ggaacatgac gcctgggcca tagtaatatg
     1981 gcaattcgcc ccgctgctgg gccgcggcca gggcagtccg gcagttcttg ctcacgtccg
     2041 gcgacttctt cacccgccca atgcggaagt gcagcgtcaa cggctgaacc tcggcattct
     2101 gctcaaaggg agcgccggac caggcggcca catcgccgtc gcccgtgcag tccacgacga
     2161 cctgggccag gatcgtcacc aggccatcct tgttggcgat ttggacgccc gtgatgcgct
     2221 gcccgctcgt ttccacgcca cacgcgaacg aattgaacag gaccgtcagc gaatcctttt
     2281 gctcccgcag caactggtca agcagcagct tgaactcgaa cgtgttagag atcgtgggat
     2341 tgtgcttcgc tactgtcttg gcatcggcgg cacacacgcc ggattttgac agcagctcca
     2401 aggcgatccc acggacaaca acccggtgat cgcgaatgct ggcaatgccg tcgaagtaag
     2461 gcagcccgac gctggtgatg atcccgcctg cgaagggtgc cttttcgacc agcaaggtct
     2521 tcgcgccttg cctggccgcc gccaaggcag ccgcggttcc cgcacatccg ccaccgcaaa
     2581 tgagcacctc ggcctggagt gtgcttcgcc cccggcgcgg catttcagcc tggactacgg
     2641 cggctgaacc aacggttgcg gctgcggccg ctgtggttgc tgcctggatg aattggcgtc
     2701 gagattcgcg attccggcgc atgagaacct gccgttaatg gcgtggggca ggaagttgga
     2761 cgctcagtct acccctatca ggggctgctg gggcgcggca atcaagtctc aaatccgaac
     2821 aacttcgccc ccgttccgcc gagaatcttg tcttgatctg cagcggaaag gtgcgtcagg
     2881 tacgagcgag ctcgatcaaa agcctgccgg tagctttcgc cggtcgcctc ggcactgaac
     2941 ccgccgccat agatcatccg ctcggcgccc caggcgtcgg tcaactcgcg aatcacgggg
     3001 gcgatatcgc ggtgcgggta cgtcgtcttg tcgggaatgg ccgagaactt catcaccgta
     3061 ttctcaaagc gcgcccagcg gaccacgacg gcgtgctcgc tcggcgtccc ctgaaatggc
     3121 cgcccgagat ggtcgatgat gactcgcgtc ttggagaatt cgcggatcag cggctcaaag
     3181 cctggtgcgt accgcggctc gaagtgcagc tgcacggcca ggccgtgatc ggtggccatc
     3241 gtccaaagcc gccgcaactc cggcttgcca aagggcggca gacggtccgg agcataggcg
     3301 tggacgcgga cgctgacgac atccagcttc cggcacagct cgggcatctt gtcgagcgat
     3361 ccctcacggt cggcaaacag caacagcgtc cctctgagct tccccttgcc gaccgacagg
     3421 caatgctcca ggtagcggtg gtcgtcctga tacggctcgg gatgaacgac aatcgcaaag
     3481 tccacaccgg ccttctccat gcagtcgagc aagtgctcgg gtgtggccgg cttgtcgggc
     3541 ccgtaaggcg cccgcgcgtg gtaggggaaa cggcgatcgt ccttcccggc aaagcaatgc
     3601 aggtgggtgt cgatacgtgg agcgcgcctt gatgtttcgg cagaaacgga gcggaaggcc
     3661 gcggcggcgg acccggcggc agcgccttgg agaacctgac ggcgggaaag cgggattgtc
     3721 atacgtggag tccggtcgaa gtcatgcccc acgctcactc cggagtaagc ccgttcggcc
     3781 tggtccagcg gatgcgggcc agcagcagat cgcctttgta accgtgtttg ggcagcattt
     3841 caccatccgt cacccacgat tcgtccggac tcacgttcac cggatgaaaa ttgccgctat
     3901 aggccacgtc atcgggggcg ttgacgccat cgcccaccag gggcaaaaca gtcctctccg
     3961 tatcccggag cagacggagt ttgtccggat cgacctgcgc catgaagagc ggcgttcgcc
     4021 agcgcggcac gttgatgttg ctggcgtcct tgcgggtata caccaggaac agggcctcgg
     4081 aatgcgccag ccagtgctgc tgcgtcgaag acatgctcag cggttcgcca tcgtcccagg
     4141 accacggctg cttgtcggag aagtcgaggc cgtcgtcgct tgccgccaca tagccgcggc
     4201 tgtcttccgc ccgcagcgtt acatagaact tgtcccgcca atgaaccagc gaaggttcta
     4261 agaggccgcg gccggccgcg tgccgcattt ccagtccaac cttttggatc gtcagcgtct
     4321 ggccgtcgaa tgagcaccgc acccccgccg ccgaacggct ggtaggcttc gcgccgaacg
     4381 acataaccaa ggcgatgtcg ccgctgggaa gtaccacccg ctggccgcag ttgttggtat
     4441 agatgagtga cccgcgaggg tcgtcccatt ccagtttccg ccgctcgccc caccggccgt
     4501 ccttccagac ggcgtagacg ggccagcgcg gcggctgctc ccgctcgaac cttggaccgc
     4561 ggtagcagac gctgtgtccc atcgccagca ccgcaccggt cgccggatga aactcaggga
     4621 cgacgtcgca aacgccctct tcccagccgt cctggcgctt aacgcgcccc agcgcaggaa
     4681 cgagttgcgg atcggtccag gtctgcccca ggtcgcgaga cgacatccaa tgcactggtc
     4741 cgaagtaatc cgacccagcg atcgtttgca acgtcatcag cgccagcggg ccgtctgctg
     4801 tcggcacgat gcacgcccgc ggatgaaacc acgtgggccc gctgccgtcc cggccgcggc
     4861 gcagcacgac cttctcgatg ctggcgacca gtcccttctc gtcagcgcaa ttggccgtag
     4921 gcatcgtcat tgcggcggca gacaatccag cggccgacga ggcaaggaac ttccgacgcg
     4981 agggcctgca ggtgcgcata gctagcgatt ccctgaccgg agttgccgcc gcaactccaa
     5041 cgggtagtcg gtgagaatgg catcgatccc caactctacc gccttggccc agtttttgcc
     5101 ctggttgccg gcaaccagcg gtccggcgag gaagatccgc ttgcctgccc gatgaagtcg
     5161 ctccacagtt tcccgcggcg gaagaaatcg cagatagacc caatcagccg ttttgtcgtt
     5221 gatggcgtct gggatctggt cgagttctgc cgccagccgg gcggcatggc atcgagcgct
     5281 ggcagcctgc agatgctccc gcaccatcgc cgattcgatc gccgcgccga taaagaccaa
     5341 ccggtcgaga accttgtggt cttctgccga gtggaccagc atctcgtcga tcccg
//
LOCUS       DASUGB010000881         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394 k141_97301,
            whole genome shotgun sequence.
ACCESSION   DASUGB010000881 DASUGB010000000
VERSION     DASUGB010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97301"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFV87_16765"
     CDS             <1..359
                     /locus_tag="VFV87_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105474.1"
                     /translation="RTQEGKFKLAALAKIDRWYLEQYVYLLQKLKATQEGGSSLLDNS
                     MIMYGSGLAWGRLHNRENLPILLAGGGGGTIEAGRHVRYGNLPLANLFLSLLDRVGVQ
                     LDRVADSTGRLPHLTA"
     gene            416..913
                     /locus_tag="VFV87_16770"
     CDS             416..913
                     /locus_tag="VFV87_16770"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5105475.1"
                     /translation="MKNHVSLRPTVELSQIGFLLGRAYDSYMGFLEHLLEESGLGDHI
                     KPGMGSLLFALFREDDRTISEVARELQLARSTMTGMVARMKKARLINVVRCRQDSRAL
                     RLRLTPLARSLEPRCQQLASRVERILSGDLSAEESRNLRRLLTIVIGTINAGLGSPRR
                     EPVAP"
     gene            complement(966..2009)
                     /gene="hypE"
                     /locus_tag="VFV87_16775"
     CDS             complement(966..2009)
                     /gene="hypE"
                     /locus_tag="VFV87_16775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX5105476.1"
                     /translation="MNWSPICPVGIDDSERILLAHGEGFRLSRRLVRERIVSRFDNRL
                     LAPLADAACLPQIAGVPAFTTDSFVVTPLFFPGGDIGTLAVFGTANDLAVVGARPRWL
                     ALSLILEEGLPLVTLERVLDSIASAARSIEIEIVTGDTKVVPRGAADQLFLNAAGVGE
                     LIDPVPPGPQAIGVGDVLLVSGPIGRHGIAVLAARDNLGFEPPPRSDCGSLFGSVEAL
                     RSSGIACCALRDATRGGVAAVLHEWAAACQLTFEIDEGNLPVTDEVRGACELLGLDPL
                     FVACEGTMMIAVAPALADRTLAVLQQRPESAAASVIGRVTQRGIAPVVVRRMLGRLQP
                     LDEPAGAPLPRIC"
     gene            complement(2006..3124)
                     /gene="hypD"
                     /locus_tag="VFV87_16780"
     CDS             complement(2006..3124)
                     /gene="hypD"
                     /locus_tag="VFV87_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX5105477.1"
                     /translation="MKYLDEFRNSVLAQHLVRAIRRSCTRRWTIMEVCGGQTHGLLRY
                     GIEEELQNVVELVHGPGCPVCVTPREAIDLACRLALEPKVLLASFGDMLRVPGSRESL
                     QAIAAAGGNVQPVYSPADAVDLARRNPRRDVVFFAVGFETTAPATALAILQAEQLGLT
                     NFSLLVSHVRVQPAMEAIAEDPVSRVEGFLAAGHVCTVAGCESYKSFVMRHGLPVVVT
                     GFEPLDLLQGIRACIRQLEAGTAAVVNQYARSVLPQGNLAAQQIVERVYEVCDRPWRG
                     LGVIPAGGLRLREEWARFDAEQRYGRQFADAEECSDCRSGDVLSGRIKPPECPHFATL
                     CNPDNPLGAPMVSSEGACAAYYRYRRQPLALRLEGAGA"
     gene            complement(3121..>3362)
                     /locus_tag="VFV87_16785"
     CDS             complement(3121..>3362)
                     /locus_tag="VFV87_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX5105478.1"
                     /translation="GKVVRWVDRDPVLAQAEVEFGGVRRVCLMACVPEAAEGDYVIVH
                     AGVAISRVDAAAAERTLVELAELSEDDGWRGEDVA"
BASE COUNT          659 a         1097 c         1031 g          575 t
ORIGIN      
        1 cacgcacaca ggaagggaag ttcaagctgg cggcattggc gaagatcgac cgctggtatc
       61 tcgagcagta cgtttatttg ctgcagaagc tgaaggccac gcaggagggc ggttcctcgc
      121 tcctggacaa ctcgatgatc atgtacggca gcggcctggc ctggggccgg ctgcacaatc
      181 gcgagaacct gccgatcctc ttggccggcg gcggaggcgg cacgattgaa gccggccggc
      241 acgtgcgcta tggcaacttg cccctggcca acctgttcct atcgctgctc gaccgtgtcg
      301 gcgtccagct cgaccgcgtc gccgacagca ccggaaggct gccgcacctg actgcttgaa
      361 acgcgaatcc agtgcgcggc agttcttcgg atggccagca atttaggatt tctccatgaa
      421 gaaccacgtt tcactgcggc ccacggtcga actctctcag atcggctttc tgctcggtcg
      481 cgcgtacgac tcctacatgg gcttcctcga gcaccttctc gaggagtccg gactaggaga
      541 tcacatcaaa ccgggcatgg gcagcctgct attcgccctg ttccgcgaag acgatcgcac
      601 gatttcggag gtcgcccgcg agctgcaact ggcgcgctct acgatgacag gcatggtggc
      661 gagaatgaag aaggcacgcc tgatcaacgt cgtcagatgc cggcaggact cccgggcact
      721 gcggttgaga ttgactccct tagcaaggtc gctggagcct cgctgccagc agctggctag
      781 ccgcgtcgag cgcattctct caggcgatct cagtgcagaa gagagccgga acctgcgtcg
      841 tttgctgacg atcgtgatcg gcacaatcaa tgcggggctt ggctcgccgc gccgcgaacc
      901 agtggcgccc tgatatgctg ccggtgtgga atgtcctaaa tgccagcctc cagaccggcc
      961 gctactcagc agattcgcgg cagcggcgcg cccgcgggtt catcgagcgg ctgcaagcgg
     1021 cccagcatcc ggcgaacgac gactggcgcg atcccgcgtt gagtcacgcg accaatgacg
     1081 ctggctgctg cagactcggg gcgctgttgc aatacggcaa gcgtacgatc ggcaagtgcc
     1141 ggcgcgacgg cgatcatcat ggtcccctcg caagcgacga atagcgggtc aagcccgagc
     1201 agttcgcacg cgccgcgaac ttcgtcggtc acgggcaagt tcccctcatc gatttcgaat
     1261 gtcagttggc aggctgcggc ccattcgtgc aggacggcgg ctacgccccc gcgggtcgcg
     1321 tcacgcagcg cgcagcaagc gatgccactg cttcgcagcg cctcgacact gccgaacaac
     1381 gaaccgcaat cgcttcgggg cggaggttcg aacccgaggt tgtcacgggc ggccagcacg
     1441 gcaattccgt gtcggccgat tggcccactg accagcaaca cgtctccgac gccaatcgcc
     1501 tgcggaccag gcgggacggg atcaatgagc tcgcccacac cggctgcgtt caggaagagc
     1561 tgatcggcag ctccccgcgg gacgaccttc gtgtcgccgg tgacgatttc aatctcgatg
     1621 ctccgagcag ccgaggcaat gctatccaag acgcgttcga gcgttaccag cggcaaccct
     1681 tcttcgagaa tgagcgacag ggccaaccac cgcggccgag cgccaacgac cgccaggtcg
     1741 ttggccgttc cgaacaccgc cagggtgccg atgtcgccgc caggaaaaaa gagcggcgtt
     1801 accacgaagc tgtcggttgt gaacgctggg acgccggcaa tttgcggcag gcaggctgca
     1861 tcggcgagcg gcgcgagcag gcggttgtcg aaccgagaaa cgattcgctc gcggaccaag
     1921 cggcgcgaca gtcggaatcc ttctccatgt gccagaagaa tccgctccga atcatcgata
     1981 cccacgggac aaattggcga ccagttcatg caccggctcc ttccagtcgg agcgccagcg
     2041 gctgccggcg gtagcgataa tacgccgcac aagccccttc gctggaaacc attggcgcac
     2101 caagcggatt gtctggattg cacaacgtgg cgaaatgtgg gcattcaggc ggcttgatgc
     2161 gtccagacag gacatccccg ctgcggcagt cggagcactc ttcggcatct gcaaactgcc
     2221 ggccatagcg ttgttcggcg tcgaacctgg cccactcttc gcgcaggcgc agaccgccgg
     2281 ctggaatgac acccagtccg cgccacgggc ggtcgcaaac ttcatagacg cgttcgacaa
     2341 tctgctgcgc ggccaagttt ccctggggca ggacgctccg cgcgtattgg ttgacgaccg
     2401 cggcggttcc cgcttcgagc tgcctgatgc aggcccggat accttgcaac aaatcgagcg
     2461 gttcaaagcc tgtcacgacg accggcaatc cgtgacgcat aacgaacgac ttgtacgact
     2521 cgcagccagc gaccgtgcaa acatgtcccg ccgccaggaa gccctcgacc cgggagacgg
     2581 gatcttcggc aatggcttcc atcgccggct gcacgcgaac gtggctcaca agtaggctga
     2641 aatttgtcag gccgagttgc tcggcctgca atatggcaag ggctgtggcg ggcgccgttg
     2701 tctcgaagcc gacagcaaag aagacgacgt ctcgacgtgg attgcgccgt gccagatcga
     2761 cggcatcagc gggtgagtaa accggctgca cgtttccgcc tgcggccgcg atggcttgaa
     2821 gcgactcgcg gcttcctggc acgcggagca tgtctccaaa gctcgcgagc agcaccttcg
     2881 gttcgagcgc caggcggcaa gccagatcaa tcgcttcgcg cggcgtcacg caaaccgggc
     2941 accccggacc gtgaaccaat tcgacgacgt tctgaagctc ctcctcgatc ccataccgca
     3001 ataagccgtg tgtttgccca ccgcaaacct ccatgatcgt ccagcgccgg gtgcaagagc
     3061 ggcggattgc gcgcaccaga tgttgggcca agacagaatt gcggaattca tccaggtact
     3121 tcatgcgacg tcttcccctc gccagccgtc gtcctcgctt agctctgcca gttcgaccag
     3181 cgtgcgctcc gctgcggcgg catccacgcg gctgatggct acgccggcat ggacgatcac
     3241 atagtcgccc tcggcagcct ctggtacgca ggccatcagg cagacgcgcc tcactccccc
     3301 aaactcaact tccgcctgag cgaggaccgg atcgcgatcg acccaacgca cgactttgcc
     3361 gg
//
LOCUS       DASUGB010000882         3910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1486399, whole genome shotgun sequence.
ACCESSION   DASUGB010000882 DASUGB010000000
VERSION     DASUGB010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486399"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFV87_16790"
     CDS             complement(<1..244)
                     /locus_tag="VFV87_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5105479.1"
                     /translation="MSSSAALDARQAVAAVYREESRRVLATLIRLLGDFDLAEEALHE
                     AFVSAVEQWPQEGVPANPRAWLVSAGRFKAIDAIRRR"
     gene            406..2136
                     /locus_tag="VFV87_16795"
     CDS             406..2136
                     /locus_tag="VFV87_16795"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5105480.1"
                     /translation="MQRGFISWVLLIVGFVVLQWPPLSRGQEAPAWPKGEVTKHAFEA
                     SKIFPGTFRDYWVYVPKQYDPAKPACVYVNQDGIQYKAPEVFDELIHKGEMPVTIGVF
                     VMHGRVKAQNDQALDRFNRSYEYDGLGDNYARFLLEELLPEVEKLKTEDGREIRLSKD
                     GNDRCIAGSSSGAICAFTAAWERPDAFRRVFSAIGTYVGLRGGNNYPTLVRKYEPKPI
                     RIFLQDGSNDQNIYGGDWWMANQELERALAFAGYEVNHEWGDGGHNGQHAAKLFPDAM
                     RWLWKDWPQPVKAGLGSPQLQDILIPGEDWKLVAEGYKFTEGPAVNAQGEVFFNDVPS
                     SKTYKIDLKGQLSEFLANSNKGDGQRFGPGGQLYAAAGGIEQILAYDAEGKSTQIADG
                     FRGNDLVVRHDGSIYVTDPFGTPNNSKVWYISKSGEKRVVDSGLKFANGICLSPDQSL
                     LYVADSRTHWVYSYQVQPDGALAHKQKYYHLHLPDTADDSGADGLRVDRAGRLYVATS
                     LGLQVCDQAGRVNCIIPTPNGKISNLTFGGEKMDTIYATCGDRVYSRRVKVAGAPSFL
                     PPHKPAAPRL"
     gene            2262..2648
                     /locus_tag="VFV87_16800"
     CDS             2262..2648
                     /locus_tag="VFV87_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105481.1"
                     /translation="MARVFGIAAVGLVYLALVNVGQAVEDPTGTWKWSVTRGDQTREQ
                     TLKLKLEGDKLTGSMPGRNNTETAIEDGSFKDNVVSFTITREFNGQKVTSKYSGTVSG
                     DEIKGKIESDRGGKTNSRDWTAKREK"
     gene            2763..3101
                     /locus_tag="VFV87_16805"
     CDS             2763..3101
                     /locus_tag="VFV87_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105482.1"
                     /translation="MPTVNELYKEHEQLKRDGKLDEAIGKLEELLKQDEKHVLAHLAL
                     AVLYGKVGKHDLAVAHGEKAVEIEPQDTFNWTALSTTYQRAFEGTQDRQYIYRAEDAK
                     MRSAQMQWQR"
     gene            3143..3577
                     /locus_tag="VFV87_16810"
     CDS             3143..3577
                     /locus_tag="VFV87_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105483.1"
                     /translation="MTWYQDLAPCDYFGPEATDSLRAVGWLAKEQDFPIGRVKLAVFR
                     RLQELLRDPWQPGTFRGFHDCELCSYEAEGRGWKNLFVPGDGVLYVTPDSILHYMNAH
                     GYRPPDEFCNAVLACPHMGTQEYRRALVKAGCGKLLKRHVDD"
     gene            complement(3596..>3910)
                     /locus_tag="VFV87_16815"
     CDS             complement(3596..>3910)
                     /locus_tag="VFV87_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5105484.1"
                     /translation="WVARDLFREHYRRYAEEAARHVYDPAAIGCVLLYTSHATMLDDL
                     ISRPLDEGDVLSNANTLIYMGKIREGDRIGRGLYIAKHRGSACDERIVAYRIDDGGVR
                     ID"
BASE COUNT          812 a         1173 c         1264 g          661 t
ORIGIN      
        1 cccgtcggcg gatggcgtcg atggccttga accgcccggc ggaaacgagc caggcccgcg
       61 gattggccgg cactccctcc tggggccatt gctcgacggc cgagacaaac gcctcgtgca
      121 gggcctcttc cgccaggtca aaatcgccca gcaggcgaat gagcgttgcc aggacgcggc
      181 gcgactcttc gcgatagacg gcagcgaccg cctggcgggc gtcgagggca gcagaggagc
      241 tcatacggca ccggcgcgag tggtgttcgt gtgattagca tcgattatag caattgcacc
      301 cctgcggtcc agcgggcggc tgtggcgagt ggcaagactc gctgcgggag ggtacatata
      361 ggctcgaatc tttggaccgg acatttcttg agctggagaa ttcctgtgca acgcggcttc
      421 atttcctggg tcctgctgat cgtcggcttc gtggtgctgc aatggccgcc gctttcccga
      481 gggcaagagg cgccggcgtg gccgaaaggc gaagtgacca aacacgcgtt cgaggccagc
      541 aaaattttcc cgggaacttt ccgcgactac tgggtctatg tgcccaagca gtacgacccc
      601 gcgaagcccg cctgcgtgta cgtcaatcag gatggcattc aatacaaagc gccggaggtg
      661 ttcgacgagc tgattcacaa gggcgaaatg ccggtgacga ttggcgtgtt cgtgatgcac
      721 ggccgggtga aagcacaaaa tgaccaggcg ctcgaccgct tcaaccgcag ttacgagtac
      781 gacggactgg gcgacaacta cgcccgcttc ctgctggagg agctgctgcc ggaggtggag
      841 aagctgaaga cggaggacgg gcgcgaaatt cgcctctcca aagacggcaa cgatcgctgc
      901 attgccggca gttccagcgg ggcgatttgc gcgttcacgg cggcctggga gcggcccgac
      961 gcttttcgcc gcgtgtttag cgcgattggc acctatgtcg gcctccgcgg cgggaacaac
     1021 tacccgacgc tcgtccgcaa gtacgagccc aaaccgatcc gcattttcct gcaggacggc
     1081 agcaacgacc agaacatcta cggcggcgac tggtggatgg cgaatcagga actggaacgg
     1141 gcgctggcct tcgccggcta tgaggtaaac cacgagtggg gcgacggcgg ccacaacgga
     1201 caacacgcgg cgaagctctt tcccgacgca atgcgctggc tgtggaagga ctggccccag
     1261 ccggtgaagg cgggcctcgg ttcgccgcag cttcaggaca ttctgattcc gggcgaagac
     1321 tggaagctcg tcgccgaagg ctacaagttc accgaaggtc ccgcggtgaa cgcccagggc
     1381 gaagtctttt tcaacgacgt gcccagcagc aagacgtaca agatcgacct caagggccag
     1441 ctcagcgagt tcctcgcgaa cagcaacaag ggggacggac agagattcgg tccaggcgga
     1501 cagctttacg cggctgccgg cggcatagag cagattctgg cctacgacgc cgaaggcaag
     1561 tccactcaaa tcgccgacgg ttttcgcggg aatgatctcg tcgtgcggca cgacggaagc
     1621 atctatgtga ccgacccgtt cggcacgcca aataacagca aggtctggta catcagcaaa
     1681 agcggcgaga agcgggtggt cgattcgggg ctaaaattcg ccaacggcat ctgcctctcg
     1741 cccgatcagt cgctgctcta cgtcgccgac agccgcacgc actgggtcta tagctaccag
     1801 gtgcagccgg acggcgcgct ggcccacaag cagaagtact atcacctgca cctgcccgac
     1861 acggccgacg acagcggcgc cgatggcctg cgcgtcgatc gggccggccg gctgtatgtc
     1921 gccacgagcc tcggcctgca ggtctgcgac caggcgggcc gcgtgaactg cattatcccg
     1981 acgcccaacg gcaagatttc caacctgacg tttggcggcg agaagatgga cacgatctac
     2041 gccacctgcg gcgaccgcgt ctattcccgc cgcgtgaaag tcgccggcgc gccttcgttc
     2101 ctgccgccgc acaagcccgc cgccccgcgc ttgtagagca gaacgtgacg gaacattgct
     2161 atcgccgcca ggtcggctac gatacgtgct ggcaaaccag cggccctccg gcgagtagcc
     2221 gagaatacgg gccgctcgca attacacacg ggggaattgt catggcaagg gtattcggaa
     2281 tcgcggccgt tggcttggtc tatctggcgc tggtgaacgt gggccaggcg gtggaagatc
     2341 cgaccggaac gtggaagtgg tcagtcacgc gcggcgatca gacgcgcgag caaacgctca
     2401 agctgaagct ggaaggcgac aagctcaccg gctcaatgcc cggacgcaac aacacggaaa
     2461 cggcgatcga ggacgggtcg ttcaaggaca acgtcgtctc gttcacgatc acgcgcgaat
     2521 tcaacggcca gaaagtgacg agcaagtaca gcggcaccgt cagcggcgac gagatcaagg
     2581 ggaagatcga gtcggatcgc ggcggcaaga cgaattcccg cgattggacc gccaagcgcg
     2641 agaagtaggc cttctgtcgc aggcgctatc actagacgcc gggaggccgc acccctcccg
     2701 gcgttgctat tggtatgatg agagccaatc aactgccccg ctcgccaaat aggtgccatg
     2761 ccatgcccac ggtcaacgaa ctctacaaag agcacgagca gctcaagcgc gacgggaagc
     2821 tggacgaggc gatcggcaag ctcgaagagc tgctgaagca ggacgagaag cacgtcctgg
     2881 cccatttggc tttggcggtg ctctacggca aagtcggcaa gcacgacctg gcggtggccc
     2941 acggcgagaa ggcggtcgaa atcgagccgc aagacacgtt caactggacg gctctcagca
     3001 cgacctacca gcgggcattc gaagggacgc aggaccgcca atacatctat cgtgccgagg
     3061 acgccaaaat gcgctcggcc cagatgcagt ggcagcgcta acgtggggta ggcgtctcgc
     3121 ctgcccgtca gtagaggctg cgatgacttg gtaccaggat ctcgctcctt gtgattattt
     3181 tggaccggaa gccaccgatt cgcttcgcgc cgttggctgg cttgctaaag aacaggactt
     3241 ccccataggt cgcgtaaagt tggcggtctt tcggcgactg caggaactcc ttcgcgatcc
     3301 ttggcagcct ggcacgtttc gcggattcca cgactgtgag ttgtgttcct atgaagctga
     3361 ggggcgcggg tggaagaacc tgttcgtacc gggcgacggc gtcctttacg tgacgcccga
     3421 ctcgatcctg cactacatga acgcgcatgg gtatcgtcca cctgatgagt tctgcaatgc
     3481 tgtgctggcc tgcccacaca tgggaactca agagtatcgc cgagcgcttg tgaaggcagg
     3541 atgcggaaag cttctaaagc gacatgtgga cgactgatcc agccgcgaag ctgagtcaat
     3601 cgatccgcac gccgccatca tcaatccgat acgccacaat ccgctcgtcg caggcgctgc
     3661 cgcggtgctt ggcgatgtag aggccgcggc cgatgcggtc gccttcgcgg atcttgccca
     3721 tgtagatcag cgtgttggcg ttggagagaa cgtccccctc gtccagcggc cggctgatga
     3781 ggtcgtcgag catcgtggcg tgcgaggtgt agagcaggac gcagccgatt gcggcggggt
     3841 catagacgtg ccgggccgct tcttcggcgt agcggcggta gtgctcgcga aacaggtcgc
     3901 gggcgaccca
//
LOCUS       DASUGB010000883         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_237829, whole genome shotgun sequence.
ACCESSION   DASUGB010000883 DASUGB010000000
VERSION     DASUGB010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237829"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VFV87_16820"
     CDS             complement(<1..940)
                     /locus_tag="VFV87_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX5105485.1"
                     /translation="MAEQTGNSRSGRRLGYFLVFAATAAATAGVMYLWQNISQRKEEA
                     REHVFQVVELDESAVDPAVWGRNFPRQYDSYRRTVDTVRTRHGGSDAFQKLDEDPRWR
                     TIFAGYAFGVDYREERGHAYMLSDQDMTERVKDFKQPGACLQCHASIIPAYRETGRKA
                     GIPDDQPQEQIQKGFELVCAMPYAEARALTDGGGHKLIEHPVACIDCHDPQSVELRVT
                     RPGFLSGIKALAESNDAVPHLPSIGRWRSGDRKEPYDPNTMAPRQELRSLVCGQCHVE
                     YYFKGSEKLLTYPWHRGLKVEQIESYYEEESPQTDWK"
     gene            complement(968..1441)
                     /gene="nrfH"
                     /locus_tag="VFV87_16825"
     CDS             complement(968..1441)
                     /gene="nrfH"
                     /locus_tag="VFV87_16825"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEX5105486.1"
                     /translation="MRQSLTRTGTILAMMLGVLAGSGLYTARYAEGLSYLSDDPKACV
                     NCHIMRDHYDGWQKASHHGFATCNDCHLPHDNLIHKYYIKAENGFWHSKGFTLQDFPE
                     PIRIRPHNRAVLNNNCVHCHHDLVGQITSHAASAETTDAWNCIHCHGQVGHGPTR"
     gene            complement(1526..>2871)
                     /locus_tag="VFV87_16830"
     CDS             complement(1526..>2871)
                     /locus_tag="VFV87_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105487.1"
                     /translation="TDRSKVLEAVAERIHRGTSYREVVAALLLAGVRNVQPRPSVGFK
                     FHAVLVVNSAHLASISSPDEHRWLPIFWAIDSFKGSQARDVEEGNWTMAPVDESRVPP
                     SHLAKRAFIEAMDNWDEAAADAAVASLARNAGANELFDIFAHYGCRDFRSIGHKAIYV
                     ANAWRTLNCVGRQYSEPVLRSLAYALLNHEGGGNPARSDLDADRPGRENVARAKKLRD
                     DWQTGTVDPAATHDLLATIYGGSSSDLCDQIVETINRGVSPQSVWDAMLVGAGELLMR
                     QPGIVGLHTLTTSNATRYAFGACGDDETRKLLLLQNAAFLPLFRGAMTGRGRVGEQKV
                     EELQAAEIPADSVAALDSIFADVSGNRLDAARKTLAYARANPQPHDFVNRARTLIFLK
                     GRDAHDYKFSSAVLEDYENVSPAWRDKFLATSVFNFKGSGDGDNNLVKRTQAALA"
BASE COUNT          447 a          923 c          944 g          557 t
ORIGIN      
        1 gcttccaatc ggtttgcggc gactcctcct cgtagtacga ctcgatctgc tcgaccttca
       61 gcccccgatg ccacggatag gtcagcagct tctccgagcc cttgaagtag tactcgacgt
      121 ggcactggcc gcagacgagc gaccgcagct cctgccgcgg ggccatcgtg ttcggatcgt
      181 acggctcctt tcgatcgccg ctgcgccagc ggccgatgct cggcaggtga ggtacggcgt
      241 cgttgctttc cgccagcgcc ttgatcccgc tgagaaaccc cggccgcgtg acgcggagct
      301 caaccgactg tgggtcgtgg cagtcgatgc aggccactgg atgctcgatc agcttgtggc
      361 caccgccatc ggtgagcgct cgagcctcgg catacggcat cgcacacacc agctcgaagc
      421 ccttctgaat ctgctcctgc ggctggtcgt cgggaatgcc ggctttgcgg ccggtttcgc
      481 gataggcggg gatgatggag gcgtggcact gcaggcaagc gcccggctgc ttgaaatcct
      541 tgacccgctc ggtcatgtcc tgatcggaga gcatgtaggc gtggccgcgc tcctcgcggt
      601 agtcgacgcc gaaggcatag ccggcaaaga tcgttcgcca ccgcgggtct tcgtccagct
      661 tctgaaaggc gtcgctgccg ccgtgccgcg tccgcaccgt atcgaccgtt cggcggtaac
      721 tgtcgtactg gcgtggaaaa ttgcggcccc agacggctgg atcgacggcg ctctcatcga
      781 gttcgaccac ctgaaagacg tgctcgcggg cttcctcttt tcgctggctg atgttctgcc
      841 agaggtacat cacccccgcc gtggcggcgg ccgtggcggc gaacacgagg aagtatccca
      901 gtcgccggcc cgagcgcgag ttgcccgtct gctccgccat cttcgtctcc tgttgtgaat
      961 cgtgctttca gcgcgttggg ccgtgaccga cctggccgtg gcaatggatg cagttccagg
     1021 catcggttgt ctccgcactt gctgcatgcg aagtgatctg tccgaccagg tcgtgatggc
     1081 aatgcacgca gttgttgttg agcacggccc ggttgtgcgg ccggatgcgg atcggctccg
     1141 ggaaatcctg cagcgtgaag cccttgctgt gccagaagcc gttctcggcc ttgatgtaat
     1201 acttgtggat gaggttgtcg tgcggcaggt ggcagtcgtt gcaggtggcg aagccgtggt
     1261 ggctcgcctt ctgccagccg tcgtagtggt cgcgcatgat gtggcaattg acgcaggcct
     1321 tggggtcatc gctcagatac gacagcccct cggcataccg ggccgtatag agcccgctgc
     1381 cggcgagcac gccgagcatc atcgcgagga tcgttccagt ccgagtgagc gactgcctca
     1441 tgcttgtgct tgtagccgcg ttcgccagaa cgcgggaaac cggttcgccg gcccgcactc
     1501 tggcgagtgc ggctacggct ggaggctagg ccagggccgc ctgcgtgcgt ttgacaagat
     1561 tgttgtcgcc gtcgccgctc cccttgaaat tgaagacgct ggtggccagg aacttatcgc
     1621 gccaggcggg cgaaacgttc tcgtaatcct ccagcacagc cgagctgaat ttgtagtcgt
     1681 gcgcgtcgcg tcctttgagg aagatcagtg tccgggcccg gttgacgaaa tcgtgcggct
     1741 gcggattcgc gcgggcgtag gccagtgtct tcctggcggc atccagtcga ttgccgctga
     1801 cgtcggcaaa gatgctgtcc agggccgcca ccgaatcggc ggggatttcc gcggcctgta
     1861 gctcttcaac cttctgctcg ccgactcggc cgcggccggt catcgcgccg cggaagagcg
     1921 gcaggaaagc ggcgttttgc aagagcagca gcttgcgcgt ttcgtcgtcg ccgcacgcgc
     1981 cgaaggcgta gcgcgtggca ttgctggtgg tgagcgtgtg caggccgacg atccccggct
     2041 ggcgcatcaa gagctcaccg gcacctacca gcatcgcgtc ccagaccgat tgtggcgaca
     2101 cgccgcggtt gatcgtctcg acgatctgat cgcacaggtc gctgctgctg ccgccgtaga
     2161 tggtggcgag caggtcgtgc gtcgcggcgg gatcgacggt gcccgtttgc cagtcgtcgc
     2221 gcagcttctt ggcgcgggcc acgttctcgc ggccgggacg atcggcgtcg aggtcgcttc
     2281 tcgcggggtt gccgccccct tcatgattga gcagggcgta ggccagcgac cgcaacaccg
     2341 gctcggagta ttgccggccg acgcaattca gcgtccgcca ggcgttggcc acgtagatcg
     2401 ccttgtggcc gatcgagcgg aaatcgcggc agccgtaatg ggcgaaaatg tcgaacagct
     2461 cgttcgcgcc ggcattgcgg gcgaggctcg cgaccgcggc gtcggctgcc gcttcgtccc
     2521 agttgtccat cgcctcgatg aaggcccgct tggcgaggtg cgacggcggg acgcggcttt
     2581 catcgacagg ggccatcgtc cagttgccct cctccacgtc gcgggcctgc gagcccttga
     2641 aagaatcgat cgcccaaaag atcggcagcc agcgatgctc gtcgggcgac gaaatgctcg
     2701 ccaggtgcgc cgagttaacc accagcaccg catggaactt gaaccccacg ctcggccgcg
     2761 gctggacatt ccgcacgccg gccaagagca gcgccgcgac cacctcgcga tagctcgtcc
     2821 cccggtgaat ccgctccgcc accgcctcca agaccttgct ccgatccgtc t
//
LOCUS       DASUGB010000884         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1348245, whole genome shotgun sequence.
ACCESSION   DASUGB010000884 DASUGB010000000
VERSION     DASUGB010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348245"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VFV87_16835"
     CDS             complement(<1..1288)
                     /locus_tag="VFV87_16835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105488.1"
                     /translation="MVGIGIFEILCILVFGLMFLAVAAAVTIVIVNRATAGKPAVATP
                     ASSPAAAVAPPPAQASHLDPAVQASLVARLTQRFCPQCRSPLAADSPEGLCPACLMAG
                     AIGGFSHPRPGSLPEGEGVINGLAATTPPSGSKPPLDSAALAEIGELQQHFPQLEIVE
                     LLGRGGMGAVYKARQKNLDRMIALKVIPPDAAKDPAFAERFSREARAMARLNHPNIVT
                     VYDFGQIGELYYLLMEYVDGVNLRHTLRTGKLAPQEALAIVPAVCDALQYAHDQGVVH
                     RDIKPENVLLDRLGRVKIADFGLAKLLGKGPDDFTLTRTQQVMGTPRYMAPEQIERPS
                     AVDHRADIYSLGVVLYEMLTGELPLGRFEPPSHKVQVDVRIDQVVLRSLEKDPQRRYQ
                     RASEVKTELASAVSWPTLAASAANLPSPLVGEGPGVR"
     gene            complement(1414..2148)
                     /locus_tag="VFV87_16840"
     CDS             complement(1414..2148)
                     /locus_tag="VFV87_16840"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105489.1"
                     /translation="MPDTDQRADPQFHTTHWSLIAAAAEPSETSQAALEDLCQAYWYP
                     VYAFIRRRGRSAEDARDLTQEFFATLLEKGYLADADPERGRFRSFLLTAVARFVSKQH
                     EKAAAQKRGGGRPPLSLNFDDGETRYQHEPSHDWTAERIFERRWALTLLDRTLARLRQ
                     EHETSGKLPQFEALKVFLTGEAGTPALRQVAENLGTTEGAVKVAIHHLRQKYRDSLRE
                     EIAQTVAAQEDVDSELQALLAALRGN"
     gene            2330..3151
                     /locus_tag="VFV87_16845"
     CDS             2330..3151
                     /locus_tag="VFV87_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5105490.1"
                     /translation="MQLGLINSAWVQANQPTVYGLRKTKEIGFDTVDIFVDPLDIDIK
                     ERRLIKDECDRLELPIVSIACVAVGLIDFNPSVQRFHIDRVKKYLDLAYEYEAKNVLL
                     VLGEYIWNQEVIPPAEQWKTGVENCRKLGDYAADLGVQIALELEPFHLSLVNSALTMV
                     RFLDDCNHPAVQANIDVSHLLLANVAPEELRRLKGKAIHVHISDCDGKKHGDLPPGRG
                     VVPFEPYLREIKALGIDGAISIELEYSPEPSRITEWVTEAYESTNRLMQQAGLRG"
     gene            complement(3157..>3381)
                     /locus_tag="VFV87_16850"
     CDS             complement(3157..>3381)
                     /locus_tag="VFV87_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105491.1"
                     /translation="VALALLIVVTILNSITGYLGPTTAEQFNDSFDEETFHRFIVLHM
                     VFLPTFFVALLAVWAWWFRPTRSPVVESRR"
BASE COUNT          634 a         1088 c         1058 g          601 t
ORIGIN      
        1 ccctcacccc cggcccctct cccacgaggg gagaggggag atttgcggcc gaggccgcga
       61 gcgtcggcca agaaacggcg gaagccagct cggttttcac ctcgctggcc cgctggtagc
      121 gccgctgcgg atctttttcc agcgagcgga gcacgacctg gtcgatccgc acatcgacct
      181 gcaccttgtg cgacggcggc tcgaagcggc ccagcggcaa ttcgccggtg agcatctcgt
      241 acagcacaac accaagcgaa taaatgtcgg cccggtgatc gacggcgctg ggccgctcga
      301 tctgctccgg ggccatgtag cgcggcgtcc ccatcacttg ctgcgtgcga gtcagcgtga
      361 agtcgtccgg tcccttgccg agcagcttag ccaggccaaa atcggcgatc ttgacgcggc
      421 ccaggcggtc gagcagcacg ttctccggct tgatgtcgcg atgcacgacc ccttgatcgt
      481 gggcgtattg cagggcgtcg cagacggccg ggacgatggc aagggcttcc tgcggcgcga
      541 gcttgccggt ccgcagcgtg tgccgcaggt tcacgccatc gacgtactcc atcagcaggt
      601 aatacagctc gccgatctgg ccgaagtcat acaccgtgac gatgttcgga tgattcagcc
      661 gggccattgc ccgcgcttcg cggctgaagc gctcggcgaa tgcggggtcc ttggccgcat
      721 cgggcggaat gaccttcagg gcgatcatgc ggtcgagatt tttctgccgg gctttataga
      781 ccgcgcccat gccgccgcga cctagcaatt caacgatctc aagctgtggg aaatgctgct
      841 gcagctcgcc aatctcggcc agcgccgcgc tatccagcgg cggtttggag ccggaagggg
      901 gcgtggtcgc cgccaggccg ttgattaccc cctctccctc tggcaaagag ccggggcgag
      961 ggtggctgaa cccgccgatc gcccctgcca tcaagcacgc cgggcagagt ccttccggcg
     1021 aatcagccgc caagggcgag cggcactgcg ggcagaaccg ctgcgtcagg cgagcgacca
     1081 gactcgcctg gaccgccgga tcgaggtgcg acgcttgcgc tggcggcgga gcaacggccg
     1141 cggccggcga gctggccggc gtcgccacag cgggtttgcc cgcggtcgcg cggttcacga
     1201 tgacgatcgt caccgccgcg gccacggcca ggaacatcag cccgaagacc agaatgcaca
     1261 ggatttcgaa gatgccaatg ccgaccataa atcacctgct gcgttatgcg cgcaatccca
     1321 gcgggaatca agcaatcgcc tcaaagccgc gaccattggt cgcgtttaca ccttggactt
     1381 acgaatgtcg cccatgaggt tacaacgtgc ggtctagttt ccccgtagcg cggccaggag
     1441 cgcttgcagc tcgctatcca catcttcttg cgcagcaacc gtttgcgcga tttcttcgcg
     1501 gagcgaatcg cggtacttct gccgcaggtg gtggatggcg actttgaccg ccccttcggt
     1561 cgtgccgagg ttctccgcaa cttgccgcag ggccggcgtt ccggcttcgc cggtgaggaa
     1621 gaccttgagg gcctcgaact gcggcaactt gccggacgtt tcgtgctcct ggcggaggcg
     1681 ggcgagcgtc cggtcgagga gcgtgagcgc ccagcggcgc tcgaagatgc gttcggcggt
     1741 ccaatcgtgc gacggctcat gctggtagcg ggtttcgccg tcgtcaaagt tgagcgacag
     1801 cggcggccgt ccgccgcccc gcttctgggc ggccgccttc tcgtgctgct tgctcacgaa
     1861 gcgggccacc gccgtcagca gaaacgagcg gaagcggccc ctctctgggt cggcatcggc
     1921 caggtagccc ttttccagca gcgtggcgaa aaactcctgc gtcagatcgc gggcatcctc
     1981 agccgagcgc ccccgccggc ggatgaaggc atagaccggg taccaatacg cctggcacag
     2041 gtcttcgagg gccgcctgcg acgtctcgga aggctccgca gccgccgcga tcaagctcca
     2101 atgcgtcgtg tggaactgag gatcggcgcg ttggtcggtg tcaggcataa ccggcagtat
     2161 agtcaatcgc tccacgcagt atccggcatt cctggcgctc actttgcaat ttgcaaattg
     2221 catttaccaa tttgcatttg ccggcccgct cgattgcaaa ttgacaattg cagattgcaa
     2281 atcgacaatc tgaacttgcc tgtcctctgt ccactgtccc ctgtctccta tgcaactcgg
     2341 cctcatcaac tccgcctggg tccaagccaa ccagccgacc gtctatggcc tgcgaaagac
     2401 caaggagatc ggctttgaca cggtcgatat cttcgtcgat ccgctggaca tcgacatcaa
     2461 ggagcggcgg ctgatcaagg atgagtgcga ccgcctggag ctgccgatcg tcagcatcgc
     2521 ctgcgtggcg gtagggctga tcgacttcaa cccgagcgtc cagcggttcc acatcgatcg
     2581 ggtgaagaag tatcttgacc tggcgtacga gtacgaagcg aagaacgtgc tgctcgtcct
     2641 gggcgagtac atctggaacc aggaggtcat cccgcccgcc gagcagtgga aaacgggggt
     2701 cgagaactgc cgcaagctgg gcgactatgc ggcggacctg ggcgtgcaga tcgccctgga
     2761 gctggagccg ttccacctgt cgctggtcaa cagcgcccta acgatggtcc gcttcctgga
     2821 cgactgcaac caccctgcgg tgcaggccaa catcgacgtc tcgcacctcc tgctcgccaa
     2881 cgtcgctccc gaggaactgc ggcggctgaa ggggaaggca atccacgtcc acatcagcga
     2941 ctgcgacggc aagaagcacg gcgacctccc ccccggccgc ggcgtcgtcc ccttcgaacc
     3001 ctacctccgc gaaatcaaag ccctgggcat cgacggcgcc atctcgatcg agctggaata
     3061 ctcccccgag cccagcagaa tcaccgagtg ggtcaccgaa gcctacgaat cgacgaatcg
     3121 cctgatgcag caggcgggcc tgcgcggcta gcggcgttat ctgcgggatt cgacaaccgg
     3181 cgagcgagtt ggtcgaaacc accatgccca cacagccaac agggcaacaa aaaatgtggg
     3241 cagaaatacc atgtgcagta cgatgaaccg gtgaaatgtt tcctcgtcaa acgagtcgtt
     3301 gaactgctct gccgtcgttg ggccaaggta gccggtaatg ctgttgagta tggtgacgac
     3361 gattagaagc gctagcgcga c
//
LOCUS       DASUGB010000885        11000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1628048, whole genome shotgun sequence.
ACCESSION   DASUGB010000885 DASUGB010000000
VERSION     DASUGB010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628048"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1784
                     /gene="guaA"
                     /locus_tag="VFV87_16855"
     CDS             177..1784
                     /gene="guaA"
                     /locus_tag="VFV87_16855"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5105492.1"
                     /translation="MSALPSAALAPPAPAAATTAPSTAAERVLVLDFGAQYAQLIARR
                     VRENNVYCEIVRHDITPDQIRRHKPRGLILSGGPSSVYEAGAPKCDPAIFTLGIPVLG
                     ICYGMQLACETLGGKVDSAPAREYGRARCSVTRAEGLLAGVPEHFDVWMSHGDQVSRV
                     SDDWLALAKTATCPIAAVKHKTLPVFCVQFHPEVTHTPLGSHMLHNFLTAVCGCTGTW
                     KLGDFAKETIGRLRERIGKDRVICGLSGGVDSSVTAALLYQAIGLQLSCILVDNGLLR
                     KDEQAAVLAEFTNHFKADLHVVKGESRFLATLKGIVEPQEKRRRIGHAFIECFKEEAL
                     KIKDVKYLAQGTLYPDVIESGAAADGPAATIKLHHNVGGLPAELGFELVEPLRDLFKD
                     EVRKLGVELGLPEDLVWRHPFPGPGLAVRCLGEVTKERLDILREADAIMIEEIKAANL
                     YRHTSQAFAVLLPVQSVGVMGDARTYENVAALRCVNTDDFMTADWTHLPYEVLASMST
                     RIINEVKGVNRVCYDISSKPPATIEWE"
     gene            1962..2363
                     /locus_tag="VFV87_16860"
     CDS             1962..2363
                     /locus_tag="VFV87_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5105493.1"
                     /translation="MLFQVDSASRTPIYRQLTQQIREAVARGRLAPEERLPSVRDLSR
                     QLVINPNTVARVYTELEREGVLNTRPGLGVFIAQPRSDLTKKARKDRLTALADQFLTE
                     AVHLGFSADEALALVSERMAKFQFPQPIGSE"
     gene            2364..3302
                     /locus_tag="VFV87_16865"
     CDS             2364..3302
                     /locus_tag="VFV87_16865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5105494.1"
                     /translation="MPAVISTRGLTKYYGRRCVVNSLDLAVPAGSVYGLLGRNGSGKS
                     TTLKMLLGFVRPDRGSASLLGEDALNLTPETRAKVAYIAEGHPFPRWMSVDEAVRFTR
                     SFYPHWNGDLLDQILDHFALPRRAKIRKLSNGQRAQVSLALAVAPDPELLILDDPTLG
                     LDTVVRRDFLESLIQIIQREGRTILISSHILADVERVADRIGILVDGVLRVDCPTDYF
                     KTSLRKVMLEFTASPPDFPACDGLVSTWRVGNRLEVVLVGFGTRHEQLIESLEPASVD
                     VLELNLEDAFIEYTRGPRRSLPLLNVEARHDERAYA"
     gene            3280..3936
                     /locus_tag="VFV87_16870"
     CDS             3280..3936
                     /locus_tag="VFV87_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105495.1"
                     /translation="MTSALTLKELRESAGVAVLGLAGLVLVALTAIGLSPLIGMLRPS
                     RAGSIPFVDDSFGWQFPLAAGALAMALGFKQSLGDLWGDAQLFLLHRPISRRTFYVTK
                     IVVGLSLYFALTGLAILIYAVWAASSGTHASPFAWSMTVPVWITWLAVSLVYLGALLS
                     GIRPAAWMGTRLMPLAAACAIATLAAALPPLAGLAVIIAAAVALVALILFMAETRDFA
                     "
     gene            3975..5384
                     /locus_tag="VFV87_16875"
     CDS             3975..5384
                     /locus_tag="VFV87_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105496.1"
                     /translation="MLLAAVVAFGLTTVWFVLVAWLFMLMEQSRIDFDHYEQLYVRLD
                     GVPVIAKGARSGARHELLTLDRQPAKGDTYQILSPAYVAPPAKGASYVSSRDWSVRLA
                     SISDGGSPAVYWYLVHDGRTNGRAYGVGYHAVTKQVAGYFSRAGFSERLPPREAWFHV
                     KGDAALAYAVPERQYYEPYWSTWFTQPSQFLLANGKLWRIDMRKRTVAPLVDCPDAEG
                     IGWAWRTADALPQPTPGAPQQASAPRSLFVRTPETALIVDPQSGEVARYPLPAQLQGD
                     SLGVFQLADGRLLIAAYPLSLGKDQLVAWIDPAGKTTKRHVAINAPSRTSDDATVAAL
                     SAVAAPAFVWQIVMAAMLAWQKLALGQATSTSNAVGQALAVCAPGMAIVLALSLAGAV
                     AAYRRQRRFGLGGAIGWAVMVFIFGIPGWIAYRLHRTWPVLEECPACDQSSPRDRDAC
                     LECGATFPPPELKGVEVFA"
     gene            complement(5788..8385)
                     /locus_tag="VFV87_16880"
     CDS             complement(5788..8385)
                     /locus_tag="VFV87_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HEX5105497.1"
                     /translation="MAAKANKAEATPMDAAAEASRLIDELTKQASKYTYRFEEIAKLP
                     ATEELLAAAPDVQAAAVREAIERTAALRDQLGPPRPDDFRYMNLHHESGFPMAAALAA
                     TRLLRRKLPLSDDDLLFLVRRTADLEMYSGWALPQLAPLVTLLARRAADGPFNDEMQV
                     QLQRLAAGMQWCENAPERKLRKEIEDLYGDPKSLPIEAGEAWSDAAIADLAAMKKKPQ
                     ATWHALLGHCLQATGSQPAKKWSEAAAPLLAAIGEAEFRQYLARWLPLVDKPRTQLIE
                     TWSQYQPNPNLLIGEANADVLKGLVWCCAGSGEREVARLLSTLAVSAYKKVPGIGPRA
                     VKIGNACIYALGAMPGTEGLAQLALLKVKVKFGTAQKGIEKSLTAVAERLGLPRDEID
                     ELAVPTYGLEEVGLRREEMGEFTAELIVDGSGSTELRWRKADGKPQKSVPAAVKTQFA
                     ADLSELKAAAKDIQKMLPAQRDRIDSLFLAARQWPVAAWRERYLDHPLTGLVARRIIW
                     QFTSGKKRQDGIWLDGKLVDTDDQPLKLADDATVELWHPIARPTAEVLGWRDWLERHQ
                     VRQPFKQAHREIYVLTDAELRTASYSNRFAAHILKQHQYNALCAIRGWKNKLRLMVDD
                     SYPPTTRELAPWGLRAEFWVEGAGDAYGVDTNETGTFLYLATDQVRFYQVGAPQLYAH
                     AGGGGYGTGWQQGDPAEPVPLENVPPLVFSEIMRDVDLFVGVASVGNDPNWTDGGREV
                     RYGNYWSSYSFGELSATAQTRKAVLEKIIPRLKIANRCSFAERFLNVQGNLRTYKIHL
                     GSGNILMEPNDQYLCIVPGRASDVAEDKVFLPFEGDRTLSIVLSKALLLADDDKISDP
                     SITRQIKGS"
     gene            complement(8398..8793)
                     /locus_tag="VFV87_16885"
     CDS             complement(8398..8793)
                     /locus_tag="VFV87_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105498.1"
                     /translation="MPATLTIRDETLGGASFREWSLEVLSERMTVRELIRSRVYQEVQ
                     DYNQRQPAEYHGLVQPTDAERTLNGYRLKKARQIDFREQLAKAIAAFGANQVLVLIND
                     KQAESLDEEFDVAPGTEVSFLKLTLLVGG"
     gene            complement(8864..9163)
                     /locus_tag="VFV87_16890"
     CDS             complement(8864..9163)
                     /locus_tag="VFV87_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5105499.1"
                     /translation="MIEISDHPAGVVVPVRAQPGASSSGIRGEQGGALKVAVTQTAEK
                     GKANEALIDTLAEKLRLKRSQIELVSGATQRSKKVLFRSMSREELQQRIARALEE"
     gene            complement(9183..10220)
                     /locus_tag="VFV87_16895"
     CDS             complement(9183..10220)
                     /locus_tag="VFV87_16895"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5105500.1"
                     /translation="MARVLVTGATGFIGIHLTRALVNRGDRVRCMVRSPERAAVLREM
                     GVGLVTGDLDDTAGLAEATTGIDVVYHLAALTKSLGSKEMFHVNRDGTANLMRACADQ
                     PRPPVVVLISSVAAAGPVPRGDIRTEADPPMPISVYGQSKLAGEEAGEWVADQVPLTI
                     VRPGAVFGPEDTGFLQVCRVLRLLRAHGAVGFWPPPMTWIYVRDLVELLLLAAERGQR
                     VPPRGGGSPGQGRYFAAAPEYPTWAEMGRIVRPMLNRPFAPVLLIPSPIAWCVAGVNE
                     VTGRLRGKAYWVNFDKVREALATSWACSGEAARRDLGFTPQWPLAKRFEETIAWYRIQ
                     GWLGDGFWSLY"
     gene            10302..>11000
                     /locus_tag="VFV87_16900"
     CDS             10302..>11000
                     /locus_tag="VFV87_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5105501.1"
                     /translation="MPLAIKPVQTNREKLQFLHFPWEHYRGDPNWIPPLRTNQKELVG
                     YKHHPFYDDALGQTFLALRDGQPVGRVMAIVNHAHNRIHNEKRGFFGFFETIDDPEVA
                     AGLLAAARGWLRERGMTALRGPVNPSMNYEIGLLIEGFDQQPTFMMTYNKPYYQRLFE
                     EVCGLTKTQDLYAFWGHIDMVQKLDQKLYYIWEECRTRFNVTLRRMNRKRFNQEVRLF
                     LDIYNRSMAGTWGFV"
BASE COUNT         1853 a         3706 c         3402 g         2039 t
ORIGIN      
        1 agtggatgcc gggctcgcag ttctccggga ccaggtttgg tcccggctat tgcgatggta
       61 tggccttccg ccgccattcg ccaacccccg tgctggcctc gggtgttagc ggtacaatcc
      121 acgattcgcc gtggcgggcc tgatgccacg gctttttcct ttgatggatc gccaccatgt
      181 ccgctctgcc ttccgctgcc ctggcgccgc ccgcccccgc tgccgcgaca accgccccat
      241 cgaccgctgc cgaacgagtg ctcgttctcg atttcggagc gcaatacgcc cagctcatcg
      301 cccgccgcgt ccgcgagaac aacgtctatt gcgagatcgt ccgccacgac atcacgcccg
      361 accagatccg ccgccacaag ccgcgggggc tgatcctgag cggcggcccg tccagcgtct
      421 atgaagccgg ggcaccgaag tgcgacccgg cgatctttac gctcggcatt ccggtgctcg
      481 gcatctgcta cggcatgcag ctcgcctgcg agacgctggg cggcaaggtc gattcggccc
      541 ccgctcgcga atatggccgc gcccgctgca gcgtgacgcg agccgaaggg ctcttggccg
      601 gcgtgcccga gcatttcgac gtctggatga gccacggcga ccaggtttcg cgagtcagcg
      661 acgactggct cgccctcgcc aagaccgcga cctgcccgat cgccgccgtc aagcacaaga
      721 cgctgccggt cttttgcgtg cagttccatc ctgaagtcac gcacacgccg ctcggcagcc
      781 acatgctgca caacttcctc accgccgttt gcggctgcac ggggacgtgg aagctcggcg
      841 actttgccaa ggagacaatc ggccgcctgc gcgagcggat cggcaaggat cgcgtgatct
      901 gcggcctgtc gggcggcgtc gattcgtcgg tcaccgcggc cctgctctat caggcgatcg
      961 gcctgcagct ttcgtgcatc ctggtcgata acggcctgtt gcgaaaagac gagcaggcgg
     1021 ccgtgctggc ggaatttacc aaccacttca aggccgacct gcacgtggtg aagggcgagt
     1081 cgcggttcct ggccacgctc aaagggatcg tcgagccgca ggagaagcgt cgccgcatcg
     1141 gccacgcctt catcgagtgc ttcaaggagg aggccctgaa aatcaaggac gtgaaatacc
     1201 tcgcccaggg cacgctctat cccgacgtga tcgaaagcgg cgccgcagcc gacggtccgg
     1261 ctgccacgat caagctgcac cacaacgtcg gcggcctccc cgcggagctc ggctttgagc
     1321 tggtcgagcc gctgcgggac ctgttcaagg acgaagtccg caagctgggg gtcgagctcg
     1381 gcctgccgga agacctcgtc tggcggcatc cgttccccgg gccgggcctg gcggtccgct
     1441 gcctgggcga ggtgaccaaa gagcgactgg acatcctccg cgaagccgac gcgatcatga
     1501 tcgaggagat caaggcggcg aatctctatc gccacacgtc gcaagccttc gcggtgctgc
     1561 tgccggtgca aagcgtcggc gtgatgggag acgcccgcac gtacgagaac gtcgccgccc
     1621 tgagatgcgt caacaccgac gacttcatga ccgccgactg gacgcacctg ccttacgaag
     1681 tgctcgccag catgagcacg cggatcatca acgaagtcaa aggtgtcaac cgagtctgct
     1741 acgacatcag cagcaaaccg ccggccacga ttgagtggga gtgacgcgga atccgtaggg
     1801 tgggacaagg ccgccgcggc tgccggccca ccatgactcg gcgggtgggc ctgcgcttcg
     1861 ctggtcccac ccctacaact acgcattccc gaattctgca tttccctctt gatccgcctc
     1921 aaccgtacta gtagtattag tacggtaacg cgaggctcgt catgctcttt caggtcgatt
     1981 ccgccagccg tacgccgatt taccggcaat tgacgcagca aatccgcgaa gcggtcgcgc
     2041 ggggacgcct cgcgccggaa gagcggctgc cgagcgtccg cgacctgtcg cggcagctcg
     2101 tgatcaatcc caacacggtg gcccgcgtct atacggagtt ggagcgggaa ggcgtgctca
     2161 acacccggcc gggcctcggc gtcttcatcg cccagccgcg cagcgatctg acaaaaaagg
     2221 cccgcaagga ccggctgacg gccctcgccg accaatttct caccgaagcg gtacacctcg
     2281 gtttttccgc agatgaggcc ctggccctgg tttcggagcg gatggcaaag tttcagttcc
     2341 cacaaccgat cgggagcgag taaatgcccg ctgtgatctc cacgcgtgga ttgacgaaat
     2401 actacggccg gcggtgcgtc gtgaattcgc tcgacctggc cgtgcccgcc ggctcggtct
     2461 atggcctgct cggccgcaat ggctcgggca aatcgaccac gctcaagatg ctgctcggct
     2521 tcgtgaggcc cgatcgcggc tcggcgagcc tgctgggcga agacgcgctg aatctcacgc
     2581 cggaaacgcg ggccaaagtc gcctatatcg ccgaagggca cccgttcccc cgctggatga
     2641 gcgtggacga agcggtccgc ttcacgcgct cgttctatcc gcactggaac ggcgatttgc
     2701 tcgaccagat tctcgaccac ttcgccctgc cgcggcgggc caagatccgc aaactcagca
     2761 acggccagcg ggcgcaggtg tcgctggccc tcgccgtcgc gcccgatccg gaattgctga
     2821 tcctggacga cccgacgctt ggcctcgata cggtcgtccg acgcgatttc ctcgaatcgc
     2881 tcatccagat cattcaacgg gaaggacgca caatcctgat cagctcgcac attctggccg
     2941 atgtggagcg cgtcgccgac cgcatcggca tcctggtcga tggcgttctg cgggtcgatt
     3001 gcccgaccga ctatttcaag acctcgctgc ggaaggtcat gctcgaattc accgccagcc
     3061 cccccgattt tcccgcttgc gatggcctcg tcagcacctg gcgggtcggc aaccgtctgg
     3121 aggtggtgct ggtcggcttt ggcacgcggc acgagcagct catcgagtcg ctggagccgg
     3181 cgtcggtcga tgtgctggag ctgaacctgg aagacgcctt catcgaatac acccgcggcc
     3241 cgcggcggtc gctgccgctc ttgaatgtgg aggcccgtca tgacgagcgc gcttacgctt
     3301 aaggaactgc gcgaaagcgc cggcgtggct gtcctggggc tggccggtct ggtgctcgtc
     3361 gcgctcaccg cgatcggcct cagcccactg atcggcatgc tccgcccgtc gcgggcaggc
     3421 agcatcccgt ttgtggacga ctcgttcggc tggcaatttc ccttggccgc gggagcgctg
     3481 gcaatggctt tgggcttcaa gcagtcgctg ggcgatctgt ggggcgatgc gcagctcttc
     3541 ctgctgcacc ggcccatcag ccggcggacg ttctacgtca cgaaaatcgt cgtcggcctg
     3601 tcgttgtatt tcgcgctgac ggggctggcg atcctgatct atgccgtctg ggccgccagc
     3661 agcggcacac acgccagccc gttcgcctgg tcaatgaccg tgcccgtctg gatcacctgg
     3721 ctggccgtca gcctcgtcta tctcggggcg ctgctctcgg gaattcggcc ggcggcctgg
     3781 atgggcacgc ggctgatgcc gctggccgcc gcgtgcgcga tcgcgacgct ggccgccgcc
     3841 ctgccgccgc tggcgggcct ggccgtcatc atcgcagcag cagtggcgct ggtcgctctc
     3901 attctgttca tggccgaaac tcgtgacttc gcctagaggc gcctatgtcc gcgtcgcaca
     3961 attccctgcg atccatgctg ctggctgccg tcgtggcttt cggcctcacg acggtctggt
     4021 tcgtgctcgt cgcgtggctg ttcatgttaa tggagcagtc gcgcatcgat ttcgatcatt
     4081 acgagcagct ctacgtgcgc ctggacggcg tgccggtgat cgccaaaggc gcccggagcg
     4141 gcgcgcggca cgagctattg acgctcgatc gccagccggc caaaggcgat acataccaga
     4201 tcctttctcc ggcctatgtt gcgccgcccg ccaaaggcgc gagctacgtc tccagccgtg
     4261 actggtcggt ccgtctggcc tcgatcagcg acggcggctc gccggccgtg tattggtatc
     4321 tggtacacga cgggcggacg aacggccggg cctacggcgt cggctatcac gccgtcacga
     4381 agcaagtggc gggctacttc agccgcgccg gcttcagcga gcgcctgccg ccgcgagagg
     4441 cgtggtttca cgttaaggga gatgcggccc tggcctacgc cgtgcccgag cggcagtatt
     4501 acgagcccta ttggtccact tggttcacgc agccatcgca atttctactg gccaacggca
     4561 agttgtggcg gatcgatatg cggaagcgga cagtcgcgcc gctagtcgat tgccccgatg
     4621 ccgaagggat cggctgggcg tggcgaacgg ccgacgcact gccgcagccg acgccgggcg
     4681 cgccgcagca agcgagtgcc ccgcggtcgc tgttcgtccg cacgccggaa acggccttga
     4741 tcgtcgatcc gcaatcgggc gaggtggcgc ggtatccgct gccggcacag ttgcaaggtg
     4801 attcgctcgg ggtgtttcaa ctggctgacg gccgcctgct gatcgccgca tatccgctaa
     4861 gtctgggcaa agaccagctt gtcgcatgga tcgatccggc gggaaagacc accaaaaggc
     4921 atgtcgcgat caacgcgccg agccgcacca gcgatgacgc taccgtggct gccctcagtg
     4981 ccgtcgccgc gccggctttc gtgtggcaga tcgtcatggc cgcgatgctg gcttggcaga
     5041 agctcgccct gggacaggcg acttccacat ccaatgccgt tggccaggcg cttgccgtct
     5101 gtgcgccagg catggcgatc gtcctggcgc tgagcctcgc gggcgccgtc gcggcctatc
     5161 gccgccaaag gcgcttcggc ctaggcggtg cgatcggctg ggcggtgatg gtcttcattt
     5221 tcggcatccc cggatggatc gcctatcgct tgcaccgcac ctggccggtc ctggaggaat
     5281 gtcccgcttg cgaccagtcc tcgccgcgcg accgcgacgc ctgcctcgaa tgcggcgcaa
     5341 cattcccgcc gccggagctg aagggcgtcg aagtcttcgc ctgaaccgcg cggtgcaagc
     5401 accgcggcga aatgggcgat ccaaagcaga aagaaaaagg cccagccgcc gggagcgact
     5461 gggcctgata gcttgtcgcc cgcgtcgcga gcacggcgga ggatcgccgg ctgatagcag
     5521 ccgatggctg ccgcgctgcg ggcactggtg ggcgttgata ggcggggctt ggccgccacg
     5581 aggagcagcg gccagtgcgt ttgtgatcgg cccctccctg gcagcataag caccatttcc
     5641 cgcttcccgc gcacggcccg cactaccgca accgcttcac aagccgttac accttgccgg
     5701 atcggtccgc gactcgccgc tcgacgataa gttgtaacgg ccgggcaatt gctgccgccg
     5761 gtcgcactcg cgacggatcc gtgccgctca acttcccttg atctgccgcg tgattgaagg
     5821 gtcgctgatc ttgtcgtcgt cggccaggag cagggctttg ctcaggacga tcgacagcgt
     5881 gcggtcgcct tcgaaaggga gaaagacctt gtcttcggcc acgtcgctcg cccggccggg
     5941 cacgatgcac aggtactggt cattgggctc catcaggata ttgccgctgc cgaggtggat
     6001 cttgtacgtt cgcaggttgc cctgcacgtt caggaaccgt tcggcgaacg agcagcggtt
     6061 ggcgatcttc agccgcggga tgatcttttc gagcacggcc ttgcgggtct gggcagtcgc
     6121 cgagagttcg ccgaaggaat agctgctcca gtagttgccg tagcggactt cgcggccgcc
     6181 gtcggtccag ttggggtcgt tccccacgct ggccacgccc acgaacaggt ccacgtcgcg
     6241 catgatctcc gaaaagacca gcggcggcac gttctccagc ggtaccggct ccgctggatc
     6301 tccctgctgc cagccggtcc catagccgcc gccgccggcg tgggcataca actgcggcgc
     6361 gccgacttga taaaagcgga cctggtccgt cgcgagatag aggaacgtgc ccgtttcgtt
     6421 ggtatcgacg ccgtacgcgt cgcccgctcc ttcgacccag aactcggccc gcaatcccca
     6481 cggcgcgagc tcgcgcgtcg tcggcggata cgaatcatcg accatcagcc gcagcttgtt
     6541 cttccagccg cggatcgcgc agagggcgtt gtactggtgc tgcttgagga tgtgagcggc
     6601 gaagcggttc gagtagctgg cggtccgcag ctcggcatcg gtgaggacgt agatttcgcg
     6661 gtgggcttgc ttgaacggct ggcggacttg atgccgctcc agccagtcgc gccagcccag
     6721 cacctcggcg gtggggcgcg cgatcggatg ccacagctcc accgtggcgt cgtccgcgag
     6781 cttcagcggc tggtcgtcgg tatcgaccag cttgccatcc agccagatgc cgtcctgccg
     6841 ttttttcccc gacgtgaact gccagatgat ccgccgcgca accaggcccg tcagcggatg
     6901 gtcgagatac cgctcgcgcc aggccgccac cggccattgc ctggcggcca ggaacaggct
     6961 gtcgatccgg tcgcgctggg ccggcagcat tttctggatg tctttggctg cggctttcag
     7021 ctcgctcaga tccgcggcga actgcgtctt gacggccgcc ggcacgctct tctgtggttt
     7081 gccgtcggcc ttgcgccagc ggagctcggt ggagccgctt ccgtccacga tcagctcggc
     7141 ggtgaattcg cccatttcct ctctgcggag gccaacctcc tccaggccat acgtgggaac
     7201 ggccaactcg tcgatttcgt cgcgcggcag gcccaggcgc tcggccacgg cggtcagcga
     7261 cttctcgatg cccttctgag ccgtgccgaa cttcaccttc actttgagga gcgcgagctg
     7321 cgccaatcct tcggtcccag gcatcgctcc gagcgcatag atgcatgcat tgccgatctt
     7381 gaccgcccgc ggcccgatgc cggggacttt cttgtaagca ctcacggcca aagtcgagag
     7441 caggcgggcc acttcccgct cgccgctgcc ggcgcagcac cagaccagcc ccttcagcac
     7501 gtccgcgttg gcctcgccga tcagcaggtt cgggttcggc tgatactgcg accaggtttc
     7561 gatgagctgc gtgcgcggct tatcaaccag cggaagccac cgcgctaagt actggcggaa
     7621 ttccgcctcg ccgatcgccg ccagcagcgg ggcagccgct tcgctccact tcttcgcggg
     7681 ctgtgatccg gtcgcttgca ggcagtggcc gagcagcgcg tgccacgtgg cctgcggctt
     7741 ctttttcatc gcggcaagat cggcgattgc ggcatcggac caggcttctc cggcctcgat
     7801 cggcagcgac ttcgggtcgc cgtacaaatc ttcgatttcc ttccttagtt tgcgctccgg
     7861 ggcgttctcg caccattgca tcccggcggc gagacgctga agctgcacct gcatttcgtc
     7921 attgaagggg ccatccgccg ctcgcctggc gagcagcgtc accagcggcg caagctgcgg
     7981 cagcgcccag cccgaataca tctccagatc ggccgtgcgt cgcaccaaaa acagcaggtc
     8041 gtcgtcgctc agcggcagct tgcgccgcag cagccgcgtt gccgccaatg ccgccgccat
     8101 cggaaagccc gactcatggt gcaggttcat gtaacgaaaa tcgtcgggcc tgggcggacc
     8161 gagctggtcc cgcagagccg ccgttcgctc gatcgcttcc cggacagccg ccgcctgcac
     8221 gtccggcgcg gcggcaagta actcttccgt cgcagggagc ttggcgattt cttcaaagcg
     8281 atacgtgtac ttgctggcct gcttcgtcag ctcatcaatc agcctcgagg cttcagccgc
     8341 cgcgtccatc ggcgtcgctt cagccttgtt cgctttcgcg gccatcgctc cctccgccta
     8401 gccgccgacg agcagcgtca gcttcaggaa cgagacctcg gtgccggggg cgacgtcgaa
     8461 ctcctcgtcc agcgattccg cctgcttgtc gttgatcagc accagaacct ggttcgcccc
     8521 aaacgcggcg atcgccttgg ccaactgctc gcgaaagtcg atctgccgcg ctttcttcag
     8581 ccgataaccg ttgagcgtcc gctcggcgtc ggtcggctgg acgagtccgt ggtattccgc
     8641 cggctggcgc tggttatagt cctgcacttc ctgataaacc cggctgcgga tcagctcccg
     8701 cacggtcata cgctccgaga gcacctccag cgaccactcg cgaaaagagg cgccgcccag
     8761 cgtttcgtcg cgaatggtca atgtggcagg catggattca ctcggaaaat gctcagatgc
     8821 cttcgctgga atattgataa ccgaccgcgg atgcaaaagc cactcattct tccaatgccc
     8881 gggcaattcg ctgctgcaac tcctcgcgcg acatgcttcg gaagagaacc ttcttcgacc
     8941 gttgggttgc accgctcacg agctcgatct gcgaacgttt caacctaagc ttctcagcca
     9001 atgtgtcgat cagcgcctcg ttcgcctttc ccttttctgc ggtctgcgtg acggcgacct
     9061 tcagcgcgcc gccttgttcc ccgcgaatgc cgctgctgct tgctccaggc tgggcgcgga
     9121 ctggaacgac gacgcctgcg ggatggtcgc tgatttcaat cattggccca ctgccagtcg
     9181 tctcagtaca aactccagaa cccgtcgccc aaccatcctt ggatgcggta ccaggcgatg
     9241 gtttcctcaa agcgcttggc cagtggccat tgcggagtga agcccaggtc gcgccgtgcc
     9301 gcctcgcccg agcaggccca gctagtagca agcgcttcgc gaaccttatc gaaattgacc
     9361 cagtaagcct tgccgcgcag gcggccggtg acctcgttca caccggccac acaccaggcg
     9421 atgggactgg ggatcaacag gactggggca aacggccgat ttagcatcgg tcgcacgatg
     9481 cgccccatct cggcccaggt cggatactcg ggcgcggcgg cgaagtaccg gccttgtccc
     9541 ggcgaaccgc ctccgcgcgg cggcacgcgc tggccgcgct cggccgccag gagcagtagc
     9601 tccacgaggt cgcggacata aatccaagtc attggcggcg gccagaatcc aaccgcacca
     9661 tgtgccctca acaaacggag aacgcgacag acttggagga atcctgtatc ctccgggccg
     9721 aacacggctc ctggccggac gatcgtcaaa ggcacttggt cggcaaccca ttcgccagcc
     9781 tcttctccag ccagcttgct ctggccataa accgaaatcg gcatcggtgg gtcggcttcg
     9841 gtccggatat ccccgcgggg aactggaccg gcggcggcga ccgaggaaat cagcacgacg
     9901 accggcggcc gcggctggtc ggcgcaggcc cgcatcaggt tggccgtgcc atcgcgattg
     9961 acgtgaaaca tctccttcga accaaggctc ttcgtgagtg ctgccaggtg atagaccacg
    10021 tcgatgcctg tggtcgcttc tgccagcccc gccgtgtcgt cgaggtcgcc ggttaccaac
    10081 cccacgccca tctcgcgcag cactgcggcc cgctctggcg agcgcaccat gcagcgcacc
    10141 cggtcgcccc ggtttacgag cgcccgcgtc agatgaatgc caataaagcc cgtggccccg
    10201 gttacaagaa ctcgcgccat ttctcccatc agccccaata gatgccgctt tgtagctatg
    10261 atcggcagct tgtcaacacg tcgccgatat actggattcg catgccgctc gccatcaagc
    10321 ccgttcaaac gaaccgcgaa aaattgcagt tcctccactt cccctgggaa cattatcgcg
    10381 gcgatcccaa ctggattccg ccgctgcgca ccaatcagaa ggaacttgtc ggctacaaac
    10441 accatccctt ctatgacgac gccctgggac aaaccttcct ggccctgcgg gacggccaac
    10501 ccgtcggccg cgtgatggcg attgtcaacc acgcccacaa ccgcatccac aacgagaaac
    10561 gcggcttctt cggtttcttc gagacaatcg atgatcccga agtcgccgcc gggctgctcg
    10621 ctgccgcccg cggttggctc cgcgagcggg gcatgacggc ccttcgcggc ccggtcaacc
    10681 catccatgaa ctacgaaatc gggcttctga tcgaaggctt cgatcagcag ccgacgttca
    10741 tgatgaccta taacaagcct tactatcagc ggctgttcga ggaggtctgc gggctgacga
    10801 agacgcagga cctgtacgct ttctggggcc acatcgacat ggtccaaaag ctcgatcaaa
    10861 agctctacta catctgggag gaatgtcgaa cgcgattcaa cgtcaccctc cgccggatga
    10921 accgcaagcg gttcaatcag gaggtgcggc tgtttctgga tatttacaac cggtcgatgg
    10981 ccggaacctg gggctttgtg
//
LOCUS       DASUGB010000886         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_933184, whole genome shotgun sequence.
ACCESSION   DASUGB010000886 DASUGB010000000
VERSION     DASUGB010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933184"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VFV87_16905"
     CDS             complement(<1..1640)
                     /locus_tag="VFV87_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5105502.1"
                     /translation="MTARTLIPRWTVLFAVVLPVLSAAAADSSLRPNVVVILADDQGW
                     GDLSVHGNTNLSTPHIDSLAKDGALFDRFFVCPVCSPTRAEFLTGRYHLRGGVRGVST
                     GQERLDLDETTIAQVFQAAGYATGAFGKWHNGLQSPYHPNDRGFDEYYGFCSGHWGDY
                     FDPPLEHNGQFVRGQGYITDDLTSHAIAFIEQNLGQPFFCYLPFNTPHSPMQVPDEYW
                     NRFKDKELAMRNREPDREDVPKTRAALAMCENIDWNVGRVLAKLDELQLAENTIVVYF
                     SDNGPNGVRWNEGMKGQKGSTDEGGVRSPLLIRWPGKIEMGKRIEQIAGAIDLLPTLA
                     DLAGIPVTSDKPLDGRSLKPLLVGSTTKWLDRTLVSHWAGRFSLRSQRYRLDNNGQLF
                     DMQADPGQRRDISKERTEVVAQLAQELSRWKKEFRDDLGPDERPFTVGYSSSTPLPAR
                     DGAGHGNVKRSDTAPNCSYFTNWTSTDDRITWDIEVGQAGRYEAVVYYTCPAADVGST
                     IELSLGDSRVQSKVTEAHDPPLYGIEHDRVPRRAESFM"
     gene            complement(1718..3133)
                     /locus_tag="VFV87_16910"
     CDS             complement(1718..3133)
                     /locus_tag="VFV87_16910"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5105503.1"
                     /translation="MRLRFRRDFLKLSSAAAMTTIFAGPQILGQESPEGSANGMWKVG
                     LAKAVITPEKAVWLAGYGSKRVPDGKLHDLWMKALALEDHRGNRAVLITSDFQGVPRS
                     MSDRVLAQLRERFGLERSQVMFTFSHNHCGPRLGDDLIDYYPVEPDQEELVAEYTDKM
                     VTLAVEMVGKALAALAPARLQFGEGRATFAVNRRNNKEAEIPALLAAGTPLKGPVDHS
                     VPVMTVTRPSGEIAAILFGYACHPTTLSFLTWCGDYPGFAQLELEKKHPGAMAMFVNT
                     CGGDQNPLPRRSVELCQKYGHQLAAGVEQALQQPLTPVSNGLATAFELIDLPYLAVMK
                     RDDLHGFLQDTNAIKARWAARMLQRLNAGKKFEASYPYPLHAWRLGSELLWIGMGAET
                     VVDYALRFKQEFGKGAWVLGYADDMIAYIPSRRVWEEGGYEGGSNLYEYGRPALRWSG
                     EIEDRIAESVHKLVAQVRKES"
     gene            complement(3149..4039)
                     /locus_tag="VFV87_16915"
     CDS             complement(3149..4039)
                     /locus_tag="VFV87_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX5105504.1"
                     /translation="MSRCLALLIASLALVVPATLVAAEPPLSALIVDGQNNHKNWPQT
                     TQMMKKYLEDTGLFHVEVATTAPQGMDETFKPDFAKYNVVVSNYNGAPWPAQTREKFI
                     DYVKGGGGFVVVHAANNAFGDWNEYNAMIGLGGWGGRNEKSGPYVYLDDSGKLVRDEA
                     KGGGGHHGPQHPFQVIVRDPDHPVTKGMPRAWMHVNDELYDLLRGPGDNMHILATAYA
                     DKDKGGSGRHEPMIFTIEYGKGRVFHTPMGHGNDSQECVGFITTLVRGAQWAATGKVT
                     LPIPEDFPTEDKTSQRKFAH"
     gene            4324..4524
                     /gene="csrA"
                     /locus_tag="VFV87_16920"
     CDS             4324..4524
                     /gene="csrA"
                     /locus_tag="VFV87_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX5105505.1"
                     /translation="MLVLSRKRDERIVINDNIVITVVDIRGDKVRLGIEAPMDMPVHR
                     QEVFDALRREQSASSKQPPGNT"
     gene            4758..>5768
                     /locus_tag="VFV87_16925"
     CDS             4758..>5768
                     /locus_tag="VFV87_16925"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105506.1"
                     /translation="MASRALAGEAGERSFGRAKSCILLFMWGGPAHQDTWDLKPDAPA
                     EIRGQFKPISTKVPGIQIGEHFPRLAQRCDQLAIVRSMTHTNVDHTPSTHFLLTGQPP
                     PPVNDRRNHWPHMGAVLSRLGRGTSALPPFVSMRPKLENDVPRFVEESQGQFGGWMGP
                     VWDPLTIDADPSKPEYRVGELTLQEGLTKERLDERRELRQGLDGVDSAAAFAAQDANI
                     QKAYDLLTSAAEQRSAFDLTQEQQKVRERYGLNPHGQSVLQARRLVERGVPLVTIFWP
                     NDGIKNVSVYWDTHSRNFIDLKDRLMPVADQAFSALLDDLSEHGMLDETLVLWTGEFG
                     RTP"
BASE COUNT         1034 a         1826 c         1808 g         1100 t
ORIGIN      
        1 ttcatgaagg actcggcccg gcgggggacg cggtcgtgct cgattccata gagcggcggg
       61 tcgtgtgcct cggtcacctt cgactgtacg cgggaatcgc ccagcgagag ctcgatggtc
      121 gagccgacgt cggccgcggg gcaggtgtaa tagacgacgg cttcgtagcg gccggcctgg
      181 ccaacctcga tgtcccaggt gatccggtcg tcggtgctgg tccagttggt gaagtacgag
      241 cagttcggag ccgtgtcgct gcgcttgacg tttccgtgcc cggcgccgtc gcgggccggc
      301 aggggcgtgc tgcttgagta cccgacggtg aacggccgct cgtccgggcc gaggtcgtcc
      361 cgaaactcct tcttccaccg cgacagttct tgtgcaagct gggcgacaac ttcggtccgc
      421 tctttcgaaa tgtcgcggcg ctggccggga tcggcctgca tgtcgaagag ctgtccgtta
      481 ttgtccaggc gataccgctg cgaccgcaaa ctgaagcggc cggcccaatg cgagaccagt
      541 gtccggtcga gccacttggt cgtgctgcca acgagcagcg gcttgaggct gcggccatcc
      601 agcggcttat cgctcgtgac ggggatcccc gcgaggtcgg cgagcgtcgg caacaggtcg
      661 atcgccccag cgatttgctc gatccgtttt cccatttcga tcttgcccgg ccagcggatc
      721 aaaagcggtg agcgaacgcc cccttcgtcg gtcgagcctt tttgcccctt catcccctcg
      781 ttccaccgaa cgccgttggg gccgttgtcg ctgaaataga cgacgatcgt gttctccgca
      841 agctgcagct cgtcgagctt cgccaggacg cgtccgacgt tccaatcgat gttttcgcac
      901 atcgccagcg ccgcgcgcgt tttgggaacg tcctcccggt ccggctcgcg gttccgcatc
      961 gccagctctt tgtccttgaa ccggttccag tattcgtccg gcacttgcat cggcgaatgc
     1021 ggcgtattaa acggcaaata gcagaagaac ggctggccga ggttttgctc gatgaacgcg
     1081 atcgcgtggc tggtcaagtc gtcggtgatg tacccctggc cccgcacgaa ttggccgttg
     1141 tgctccagcg gcgggtcgaa atagtcgccc cagtgccccg agcaaaaccc gtagtattcg
     1201 tcgaaaccgc ggtcgttcgg gtgatagggc gactgcaagc cattgtgcca tttgccgaat
     1261 gccccagtcg catacccggc ggcttggaag acctgggcga tggtcgtttc gtccaggtcc
     1321 agccgctcct ggcccgtgga aacgccccgc acgccgccac gcaggtgata gcggccggtc
     1381 agaaactccg cccgcgtcgg cgagcaaacg gggcagacga agaaccggtc gaagagcgcc
     1441 ccgtccttgg ccagcgagtc gatgtgcggc gtcgaaaggt tcgtattgcc atggacgctc
     1501 agatcgcccc aaccctgatc atcagccagg ataacgacga cgttgggccg cagcgacgaa
     1561 tcagcggccg ccgcagaaag cacgggcagg acgacggcaa acagcaccgt ccatcgcggg
     1621 atgagcgttc gagcggtcat cggggggcag actccggctc agaaaacacg gctggacgcg
     1681 gcgattgtcc tggatacgga cgacccagtc aaatacttca agactccttg cgaacttggg
     1741 cgacgagctt gtggacgctt tcggcaatgc ggtcttcgat ctcgcccgac cagcggaggg
     1801 cggggcggcc gtattcgtac aggttcgagc cgccctcgta gccgccttct tcccagacgc
     1861 gccgcgaggg gatgtaggcg atcatgtcgt cggcatagcc caggacccac gcgcctttgc
     1921 cgaactcctg cttgaaccgc agggcgtaat cgacgaccgt ctcggctccc atgccgatcc
     1981 acagcagctc gcttcccagc cgccaggcgt gcagcggata gggataagac gcctcgaact
     2041 tttttccggc gttaagccgc tggagcatcc gggcagccca gcgggctttg atggcgttcg
     2101 tgtcctggag gaacccatgc aggtcatcgc gcttcatgac ggccaggtag ggcaggtcaa
     2161 tcagctcaaa tgccgtcgcg aggccgttgg agaccggcgt cagcggctgc tggagggcct
     2221 gttcgacgcc ggcggcgagc tggtggccat atttctggca caattcgacg ctccgccgcg
     2281 gcagcgggtt ttgatcgccg ccgcaggtgt tgacgaacat agccatcgcg cccggatgct
     2341 tcttttcgag ctcaagctgc gcaaagccgg gataatcgcc gcaccaggtg agaaagctaa
     2401 gcgtcgtcgg atggcaggca tagccgaaga ggattgccgc gatttcgccg ctgggccgcg
     2461 tgacggtcat taccggtacg gagtgatcga cgggcccttt gagcggcgtt cccgcggcca
     2521 agagcgcggg aatctccgct tccttgttgt tgcggcggtt gaccgcaaat gtcgcccggc
     2581 cttcgccgaa ctgcagccgg gccggcgcca aagcggcgag cgctttgccg accatctcga
     2641 cggcgagcgt aaccattttg tccgtatatt cggcgaccag ttcctcttga tccggctcga
     2701 cggggtaata gtcgatcaga tcatcgccca gccgcgggcc gcagtgattg tgcgagaagg
     2761 tgaacatcac ctgcgagcgc tccaatccga aacgctcgcg gagctgcgcc agcacgcgat
     2821 cgctcatcga ccgcggcacg ccctggaaat cgctcgtgat cagaacggcc cggttcccgc
     2881 ggtggtcttc cagcgccagg gctttcatcc acagatcgtg cagcttgccg tcgggcacgc
     2941 gctttgagcc atagccggcg agccagaccg ctttctcggg cgtgatgacg gccttggcca
     3001 ggccgacttt ccacattcca ttcgcactgc cctcgggcga ctcttggccc aggatctgcg
     3061 ggccagcgaa gatggtcgtc atcgcggcag cggacgagag cttcagaaaa tctcggcgaa
     3121 atctgagcct cacaaaacgc tccatgcctt aatgggcaaa cttccgctgg ctcgtcttgt
     3181 cctccgtcgg aaagtcctca ggaatcggca gcgtgacttt gccggtggcg gcccactgcg
     3241 cgccgcggac gagcgtcgtg atgaagccga cgcactcctg cgaatcgttg ccgtgcccca
     3301 tcggcgtatg gaagacgcgc cccttgccgt actcgatggt gaaaatcatc ggctcgtgcc
     3361 ggccggaacc gcccttgtcc ttgtcggcat aggcggtcgc caggatgtgc atgttgtcgc
     3421 cgggaccgcg cagcaggtcg taaagctcat cgttcacgtg catccaggcc cgcggcatcc
     3481 ctttcgtcac gggatgatcg ggatcgcgga cgatgacctg gaacggatgc tgcgggccgt
     3541 gatggccgcc gccccccttc gcttcgtccc ggacgagctt gccgctgtcg tcgagataga
     3601 cgtagggccc gctcttctcg ttccgcccgc cccagccacc gaggccgatc atcgcgttgt
     3661 actcgttcca gtcgccgaag gcgttgttcg ccgcatggac gacgacgaat ccgccgcccc
     3721 ccttcacata gtcgatgaac ttctcccgag tctgggccgg ccagggcgca ccgttgtaat
     3781 tggagaccac gacgttgtac ttcgcgaagt ccggcttgaa agtttcgtcc attccctgcg
     3841 gggcggtcgt cgccacctcg acatggaaca ggcccgtgtc ttccagatac ttcttcatca
     3901 tctgcgtcgt ctgcggccag ttcttgtgat tgttctggcc atcgacgatc agcgcgctca
     3961 ggggcggctc agccgccaca agtgtggcgg gaacgaccag cgctagtgat gcaatgagca
     4021 gtgcgagaca gcgagacata gatcggctcc ttggcaggca aaatgaccag gtggagattc
     4081 taaccggccc ggaagccaaa aaatgctgca aacttgggat cgcagcggcc gatgtatccg
     4141 caagttgttg tgccgcaatg gcttatagag aatctcgcta acctgcatcg gacccaaatt
     4201 tcacgttagc agcaccggct cgcaagcgga tgcccgcaag aacttaactt tttccacgcc
     4261 cgccctaaaa ttcgagagcc taagggttta ccgacgcgag gtctctacaa gaggcatgct
     4321 ggcatgctgg tgctatcacg gaagcgggac gaacggatcg tcatcaacga caacattgtg
     4381 atcaccgtgg tcgatatccg cggagacaag gtccgtttgg ggatcgaagc gcccatggac
     4441 atgccagtgc atcggcagga agtgtttgac gcgctccgtc gcgagcagag tgcttcttcc
     4501 aagcagccgc ctggcaatac ttgattattg ccccttggtc ggcgcctgcg ctgcggtggc
     4561 ctctttgccg cttgcgtttg attcaggcag caatctacgc taggcattag ctgctttctc
     4621 cctttgtcgc cagcgatcgg gtagatcacg tgaggccgtc aggctgccat caattccgca
     4681 catcgacgag gctctctcgg cgccagacgc tgcgcgcggg ggcgctgggc ttgttcggcc
     4741 tttcgttgcc gcaactcttg gctagccgtg cgttggccgg cgaggccggt gagcggtcgt
     4801 tcggccgggc caaatcgtgc atcctgctct tcatgtgggg cggaccggcg catcaggaca
     4861 cgtgggacct caagccggat gcccccgcgg aaattcgggg ccaattcaag ccgatctcga
     4921 ccaaagtgcc cggaatccag atcggcgagc atttcccgcg gcttgcccag cggtgcgatc
     4981 agctcgcaat tgttcggtcg atgacgcaca ccaacgtcga tcacacaccc agcacgcatt
     5041 tcctgctcac cggtcagccg ccgccgccgg tgaatgatcg gcggaatcac tggccgcaca
     5101 tgggagcggt gctttcgcgg ctgggccgcg gcacgagcgc gctgccgccg ttcgtttcga
     5161 tgcggccgaa gctcgaaaac gacgtgccgc gattcgtcga agagagccag ggccagttcg
     5221 gcggctggat ggggccggtc tgggatccgc tgacgatcga cgccgatccc agcaagccgg
     5281 agtatcgcgt cggcgagctc acgctgcagg aagggctgac gaaggagcgg ctcgatgagc
     5341 ggcgtgaact gaggcagggg ctcgatggag tggattctgc agcagccttt gccgctcagg
     5401 acgccaacat tcaaaaagct tacgacctcc tcacgtccgc tgccgagcaa cgcagcgcgt
     5461 tcgacctgac gcaagagcag cagaaggtcc gcgagcgcta cggcctcaat ccgcacggcc
     5521 aatcagtcct ccaggcccgc cggctggtcg agcgcggcgt gccgctggtc acgatcttct
     5581 ggcccaacga cggcatcaag aatgtcagcg tctattggga tacccacagc cgcaatttta
     5641 tcgacctgaa agaccgcctg atgccggtcg ccgaccaggc attctcggcc ttgctcgacg
     5701 acctgagcga gcacggaatg ctcgacgaaa cgctcgtcct ctggaccggc gagttcggcc
     5761 gcactccc
//
LOCUS       DASUGB010000887         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_377065, whole genome shotgun sequence.
ACCESSION   DASUGB010000887 DASUGB010000000
VERSION     DASUGB010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377065"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFV87_16930"
     CDS             complement(<1..180)
                     /locus_tag="VFV87_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105507.1"
                     /translation="MQRNAPPGGAGSPSADAVSKYQSKRQSLAPIVIASWGVVAILLV
                     AAGVLMYLLRQRTLAD"
     gene            complement(414..539)
                     /locus_tag="VFV87_16935"
     CDS             complement(414..539)
                     /locus_tag="VFV87_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105508.1"
                     /translation="MIVWLQAELRSRWDTPVDAQDPEKYGGVFVAPSRGIADNLN"
     gene            complement(536..2014)
                     /locus_tag="VFV87_16940"
     CDS             complement(536..2014)
                     /locus_tag="VFV87_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5105509.1"
                     /translation="MLPRSLTTIVRSLRFRLLLWNAGAVLLTGACILLAVRQGVRYTL
                     VLDLDQVLREDLKEIELHFQPGEQIDWPVLQEELNLKAQGHDYHGWFVQFYDAQDQPI
                     WSSVNTPSLPPLTAPQKRQGIFSVGDYRLSYRPLPRDGEQAVAATVGVREAFITRDMA
                     RIDRLVTTVFGALLIASPLAGWFLTNRTIQPLASMIRTTSRLRPGELEDRMPLRGTGD
                     ELDRLAQTINGLLDRISSYLEQEHDFLAHAAHELRTPLAAIRSSVEVALNGDRSETEY
                     RELLSLVIEQCSALQTLVNQLLLLAETDADRLKTDAEPVALDQVVQRAVEMFQGVAEF
                     HGITLQTKPLPPVAVAGNRHHLRQVLNNLLDNAIKFTAAKASEVGAENCSLPLPPEIT
                     VTLSSDQASGTATLTVADQGIGIDSKSLPHIFDRFYRADQVRSREGMSAGTGLGLSIC
                     RAIVEAHHGTISAESTIGRETKFIIVLPQVRSSSRKHEITAP"
     gene            complement(2035..>2354)
                     /locus_tag="VFV87_16945"
     CDS             complement(2035..>2354)
                     /locus_tag="VFV87_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5105510.1"
                     /translation="TSDRPSTVLTVGPLTLDLTTRRATRGECEINLTPTEFSLLEFLM
                     RYAGQVVTRRMLCEHLWESDWEGVTNVVEVHINRLRGKVDRGFDEPLIHTVRGRGYLL
                     KAL"
BASE COUNT          465 a          720 c          732 g          437 t
ORIGIN      
        1 gtccgcgagc gttctctgcc gcaacaagta catcagcacg ccagccgcaa cgaggaggat
       61 tgcgacgacg ccccagctcg cgatgacaat cggcgccagc gactgacgct tcgattggta
      121 cttggaaacg gcgtccgccg acggtgaccc cgccccgccc ggcggcgcat tacgctgcaa
      181 gctcggcgcc gcggtcggct gctgcggaaa tggagccggt ccaatctgcg ccccgccagg
      241 tattgccgga gacgtcgcgg aacctgcaag ctgaatcact tgccggcatg ccgggcaagc
      301 gatcgcctgc ccgaccatct ccggcgttat cgaaagtggc gcctggcacc cgggacacgc
      361 aatgacaacc gcagacatcg acatctcccg ttaagccagg aagcgtgcga ttctcaattc
      421 agattgtcgg cgatcccccg cgacggtgca acaaatactc ctccatattt ctccggatcc
      481 tgcgcatcga ctggtgtatc ccagcgactt cgcagctctg cctggagcca gacgatcatg
      541 gagctgttat ctcgtgcttg cgggaactac ttcttacttg aggcaggaca atgataaact
      601 tcgtctcgcg gccaatagtg ctctcagccg aaatagtgcc gtgatgtgct tcgacgatgg
      661 cccgacagat gctgagtcct agtcccgtgc cagcagacat cccctcgcgg gagcggacct
      721 ggtccgcgcg atagaagcgg tcgaaaatgt gcggcaggct tttggaatca atgccaattc
      781 cctgatcggc aactgttagc gtggccgtgc ccgatgcctg gtcggacgac agcgtaacgg
      841 tgatttccgg tggaaggggc aacgagcaat tctctgcacc gacttccgag gccttagcgg
      901 ccgtaaattt gatcgcgttg tcgagtagat tgttcaaaac ctgacgcagg tgatggcggt
      961 tgccagcgac ggccacgggt ggaagcggtt tggtctgcag ggtgatgcca tggaattcgg
     1021 cgacgccctg aaacatttcc acggcccgct gtacgacctg gtccagcgcc acgggctcgg
     1081 cgtcggtctt caaacggtcg gcgtcggttt ccgccagaag cagcaactga ttgacgagcg
     1141 tctgcagggc agagcactgc tcgatcacca aactcagcag ctcgcggtac tcggtttcgc
     1201 tgcggtcgcc gttcaaggcc acttccacgc tgctgcggat ggcggctagc ggcgtgcgca
     1261 attcgtgggc ggcatgggcg agaaaatcgt gctcctgctc caggtagctg gatattcgat
     1321 cgagcaggcc gttgatcgtc tgggccagcc gatccagttc gtcgcccgtc ccccgcaggg
     1381 gcatgcgatc ctccaattcg cccggccgca agcgagaagt cgtgcggatc atgctcgcca
     1441 gcggctgtat ggtgcggttg gtaagaaacc acccggccag cggagaagca atcaacagcg
     1501 ctccaaaaac cgttgtcacc agtcggtcga tgcgggccat atcgcgcgtg atgaaagcct
     1561 cgcggacgcc gacggtggcg gccaccgctt gctcgccgtc gcgcggcagc ggtcgatacg
     1621 acagacgata gtcacccacg gaaaaaatcc cctggcgctt ctgaggagcg gtcaatggcg
     1681 gcagcgacgg agtattcacg ctggaccaga tgggctggtc ttgggcgtca tagaactgta
     1741 cgaaccagcc gtgatagtcg tgcccctgcg ccttaaggtt cagttcctcc tgcaggacgg
     1801 gccagtcaat ttgctcgccc ggctgaaagt gcagctcgat ttccttgaga tcctcgcgca
     1861 ggacctggtc gaggtcgaga accagcgtat agcgcacgcc ttgccgcacc gccagcagga
     1921 tgcaggctcc cgtgagcaat accgccccgg cattccagag cagcaaccga aaacggaggg
     1981 agcgaacgat cgtggtgagc gaacgaggaa gcatgtcgct ccgctagcgc tctgtcacag
     2041 cgccttgagc agatagccgc ggccgcggac cgtgtgaatc agcggttcat caaagccgcg
     2101 atccacttta ccgcgcaggc ggttgatgtg tacctcgacc acgttggtga cgccttccca
     2161 atccgattcc caaaggtgct cgcagagcat gcggcgcgtg acgacctggc cggcgtagcg
     2221 catcagaaac tcgagcaggc tgaattcggt gggggtgaga ttgatttcgc attcgccgcg
     2281 cgtggcccgg cgggtcgtca agtcgagcgt gagcgggccg accgttaata cagtcgaagg
     2341 ccggtcgctg gtcc
//
LOCUS       DASUGB010000888         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1487330, whole genome shotgun sequence.
ACCESSION   DASUGB010000888 DASUGB010000000
VERSION     DASUGB010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487330"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..819
                     /gene="fliW"
                     /locus_tag="VFV87_16950"
     CDS             376..819
                     /gene="fliW"
                     /locus_tag="VFV87_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEX5105511.1"
                     /translation="MQIQTTHFGVLEIEVDDIIVFPRGLIAMEDCHHWILLADDDNPA
                     LAWLQCVTQANLALPVVSPRRFKADYQVHVARGQLTPLELSQFDQAYVLAVISHSDGD
                     LTLNLKAPLIINLGRRIGRQVITSDDQPVAMELASRPTAPLRKIA"
     gene            1057..1290
                     /gene="csrA"
                     /locus_tag="VFV87_16955"
     CDS             1057..1290
                     /gene="csrA"
                     /locus_tag="VFV87_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX5105512.1"
                     /translation="MLVLSRHRDESIMIGDDIVVTIVDIRGDKVRLGIEAPTAIPVHR
                     QEVYEAIQRENRKATQLKPEDTRDLGRPNPSGK"
     gene            1820..>2397
                     /locus_tag="VFV87_16960"
     CDS             1820..>2397
                     /locus_tag="VFV87_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX5105513.1"
                     /translation="MSRINTNVSSLIAQKTLGRTNVQLQESLSRLSTGLRINSGKDDP
                     AGLIASENLRRDITSINKAISNTERANQLIATTDSALAQVSNLLNDIRGLVVEAANTG
                     VLSEEQIAANQLQVDSSLEAIDRIAQVTTFQGRKLLDGSLDFQVAYTAGGTTVRDLDI
                     QQANLGTAGSVSVQVDITTAATQALISNNGFNP"
BASE COUNT          459 a          778 c          711 g          449 t
ORIGIN      
        1 ataccgacct ggccgacgcc atctcgcagc taacggcgcg ccaggccgcg ctggaagcct
       61 cgctgcagct cgccgcgcag accttccgcc tctcgctgct cgacttcctg tagtgcgggc
      121 ggacgtctcc actggcggca cgccccgagg gggtcgggag tcaattgcgg taacggtcgt
      181 ccaagtggaa cgtcgtaacc gcaactgact cccgacccct ttggcgctcc accgcctaca
      241 accgtgacgc ctgcccttcc tgtagtgcag cttttgctga ttgtgacgct cgtcaactgc
      301 cgatgacgat tgacggggct gtgccgcgcg agcgcggcaa acagagcgca ttcgtgttgc
      361 cgtgaaagtg ccagcatgca gattcaaacg acacattttg gcgtcctcga gattgaggtg
      421 gacgacatca tcgtctttcc tcgcggcttg atcgcgatgg aagattgcca ccactggatc
      481 ttgctcgccg atgacgacaa cccggccctg gcgtggctgc aatgtgtcac gcaggcaaat
      541 ctcgcgctgc cggtcgtgag tccgcgccgc ttcaaggccg actatcaggt gcatgtcgct
      601 cgcgggcagc tcacgccgct ggaattgtcg cagttcgatc aggcgtacgt gctggccgtc
      661 atcagccaca gcgacggcga tctcacgctc aatctcaagg cgccgctgat tatcaatctc
      721 ggccgccgca tcgggcggca agtgattacg tccgacgacc agcccgtggc gatggaactg
      781 gcgtcccggc ccacggctcc gcttcgcaag attgcctgat acaagcggtg cggtgcgaaa
      841 aaatgtgacg gctgcgcaaa gaggtgattc tgggcaatgc ttgcttccga tagatcacct
      901 gtgctggcag accctggcag catcaccgtg ttctccgccg gggccagccg ctgaaatccc
      961 tcccaccacg tgcggtccct gctgcacttc agccccaatc ctccgcgtcc agccccatac
     1021 gtgcaagctg gcgcggttgc aaggaaggag ccaacgatgt tggttttgtc gagacaccgt
     1081 gacgagagca tcatgattgg cgacgacatc gtggtcacga tcgtcgatat ccgcggcgat
     1141 aaggtccgcc tcgggattga agcccctacc gcgattccgg tccaccgcca ggaggtctac
     1201 gaggccatcc agcgcgaaaa ccgcaaagcg acgcagctca agccggaaga tacgcgcgat
     1261 ctgggccggc ccaatccgtc gggcaagtag atctgcagcc agcccttctt tgctgcatct
     1321 ttctaggcgg gggtcggcgt ttggccgccc cgccatctga cttaccaatt cgcgtgcgac
     1381 aatcacccga agcgtaagcg acggactccc tcgggtacgc ttcgggttga tgtgcctgtc
     1441 ggcttcaggc tgggagaagg ttccgtgcat tccggatatg ccgcgtcgca atgtcgcgtt
     1501 ctgtcggaaa tcgccgcacc ttgcctaaag catccatttt tcggcgtcga tggtgttggt
     1561 aagcgaaggc cgtttccagg cttcgtctcc ggtcgtgtga agtgccaagg atgccggaca
     1621 tgacgatccg tctcgccacc caccgggttg gcgggacgag gcgatcccaa cgatcgcagc
     1681 ggccgcggga ggtcgctggc ggctacttaa ctgtggccta gagttccagg cagtggggcc
     1741 gacgttggat ctcgtttcct gctcagtccg aatccgccgc ctccgttcac atcaccgatt
     1801 ctaaaggggt cgaaagcaca tgagccgtat taacacgaac gtcagctcgc tgatcgctca
     1861 gaagactctc ggtcgcacca acgtccagtt gcaggaatcg ctgagccgtc tgagcacggg
     1921 tctgcgaatc aattccggca aggacgaccc ggccggcctg atcgcgagcg aaaacctccg
     1981 ccgcgacatt accagcatca acaaggccat ctcgaacacc gaacgggcca atcagctcat
     2041 cgcgacgacc gacagcgccc tggcccaggt gagcaacctg ctcaacgaca tccgcggcct
     2101 ggtggtcgaa gcggccaaca ccggtgtgct cagcgaagag cagatcgccg ccaatcagct
     2161 ccaggtcgat tcgtcgctgg aagccatcga ccggatcgcc caggtgacca cgttccaggg
     2221 ccgcaagctg ctcgacggca gcctggactt ccaggtggcc tacacggccg gcggcacgac
     2281 cgtccgcgat ctggacatcc agcaggccaa tctcggcacc gccggctcgg tcagcgtgca
     2341 ggtggacatt acgactgcgg ccacgcaggc gctgatttcg aataacggct tcaatcc
//
LOCUS       DASUGB010000889         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_239231, whole genome shotgun sequence.
ACCESSION   DASUGB010000889 DASUGB010000000
VERSION     DASUGB010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239231"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..>2808)
                     /locus_tag="VFV87_16965"
     CDS             complement(132..>2808)
                     /locus_tag="VFV87_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105514.1"
                     /translation="ASKLVARILLDIQIAAAGATAAALALWAAWAIFSEPGNPPPEIA
                     TLGNLLSYLSVGIVLAVLIWVAWGRETKLSLDYAGDLIAAFLIGLAALIAASASRYDP
                     GGQWIAYHTLTAGWMLVGAAACSSVVFRSAKERADRGAIGDNLLPRHFTAAAIAGLIT
                     LLAIRGNASDPQQPWWSLAATLASFAIATALGLACRSQLYAYASTLLAGLAVILYWLA
                     PVTGPWHVAAFGNMPAVAEALVLALVAAASLWLWREIATQRRQNQSLDTWFLGPPVHV
                     AAVMAILPSYFLLRLIYAVFYNHPGWFDSLSGFSAYAALAMTATVTLGALVAAMLWDR
                     RAVVTISAAYVWGGIGWLLAIGLASHWLPEIEHRIVAIFAALALHIALTGQIWSYGAN
                     LSVIGARLGISDPVGGLARTALWLPVVSALFALAVCVVDLIFVLTLKQDYLRNVAALG
                     PAACAWGVLCLAQDRRRDAFQLGALLLAGLAAVYLAWAQIDPDHTRAVWLTRVFRLVM
                     VLAALTFVYGLALPRWLLTSGSWNAATRKAGYITAAAAVAAFVATLALEVALFQPGVN
                     TAAVDDLQVAAVAVVLLLLIAGLVSLALLPGRDPLMLSETNRQAYVYAAQAIAALLFA
                     HLYVCRPLWFDGILRPYWPFIVMGLAFVGVGTSEIFQRWNIRVLAEPLVRTGALLPIL
                     PVIGMWVVGRTDDAEILFVVGLLYLAVSFTRKSWAAMVAAALAGNGALWALLLDLNFN
                     FAAHPQFWLIPPAISVLLAAQVNRHRLQPHVLTAIRYAATVVIYVSSTSEIFIRGIGD
                     SLWPPMALLVLAVLGALAGIALRVRAFLFLGAAFTVLALVAMVAHAAQAIDHVWPWWV
                     FGISVGIAILILFGIFEKKRPEVLALISRLKQWEL"
BASE COUNT          576 a          874 c         1001 g          357 t
ORIGIN      
        1 ggacttcatc ggccgcgctc cgcagcaggt cgatgaattc ctggccgaga tcgccgagcc
       61 catccgcgag cgctaccccg gcagcgtcgc cggcggaccc tcagcggagg tgaaggtgta
      121 acggacttcc ctcacagctc ccactgcttg aggcggctga tgagggcgag gacttcgggg
      181 cgttttttct cgaagatgcc gaagaggatg aggatggcga tgccgacgct gatgccgaag
      241 acccaccagg gccagacgtg atcgatggcc tgagcggcgt gggcgaccat tgcgacgagt
      301 gccagcacgg tgaacgcggc ccctaggaac aggaatgctc gcacgcggag ggcgatcccg
      361 gcgagcgcgc cgagcacggc caaaacgagc agggccatcg gcggccagag actgtcgccg
      421 atgccgcgaa tgaagatttc gctcgtcgag ctgacgtaga tcacgaccgt cgcggcatag
      481 cggatggcgg tcagtacgtg gggctgcagg cgatggcggt tcacctgcgc ggcgagcagc
      541 acggaaatgg ccggcgggat cagccagaac tgcggatggg cggcgaaatt gaaattcagg
      601 tccaggagca aggcccacaa cgccccgttg ccggccaggg cggcggcgac cattgcggcc
      661 cagcttttgc gcgtgaagct gacggcgaga tagagcaggc cgacgacgaa caggatttcc
      721 gcatcgtcgg tgcggccgac gacccacatg ccgatgacgg gcagaatcgg caacagcgcg
      781 ccggtgcgca cgagcggctc ggccaggacg cggatgttcc agcgctggaa gatctcgctc
      841 gtgccgaccc cgacaaacgc cagccccatc acgataaacg gccagtacgg ccgcaaaatg
      901 ccgtcaaacc acagcggccg gcagacgtag aggtgggcga agagcagcgc cgcaatcgct
      961 tgagcggcat agacgtacgc ttgccgattg gtctcggaga gcatcaaggg atcgcggccc
     1021 ggcagaaggg ccagcgaaac gagaccggcg atcagaagca gcaaaacgac cgcgaccgcg
     1081 gcgacctgca ggtcatcgac cgcagccgtg tttacgcccg gctggaagag ggcgacttcc
     1141 agggccagcg tggcgacgaa ggccgccacc gccgccgctg ccgtgatgta gcccgccttg
     1201 cgggtcgctg cgttccagct tccgctggtc aacagccacc gcggcagggc caggccatag
     1261 acgaacgtga gggccgccaa aaccatcacc agccggaaga cgcgcgtcag ccacacggcc
     1321 cgcgtgtgat cgggatcgat ctgcgcccag gcgagataga cggctgccag cccggccagc
     1381 aagagcgccc caagttgaaa cgcatcgcgc cgccggtcct gagccaggca gagcacgccc
     1441 cacgcgcaag cggcggggcc gagcgcggcg acattccgca gatagtcctg cttgagcgtc
     1501 aggacaaaga tcagatcaac gacacagacg gccagcgcga atagcgcgct gacgaccggc
     1561 agccagagag ccgtgcgcgc cagtcctccg acgggatcgg agatgccgag ccgagcgccg
     1621 atcacggaga gattcgcgcc gtagctccag atctggccgg taagtgcgat gtgcagggcc
     1681 aaggcggcga atatcgcgac gatgcgatgc tcgatctccg gcagccaatg cgaggccagc
     1741 ccgatggcca agagccagcc gattccgccc caaacgtacg cggcggaaat tgtgacgacg
     1801 gctcgtcgat cccagagcat cgctgccacg agcgcgccga gcgtgactgt ggcagtcatt
     1861 gccaacgcag cataggcgga gaagccgctg agtgaatcga accagccggg atggttgtag
     1921 aacacggcat aaattagccg cagcaggaag taagacggca ggatcgccat gacggctgcg
     1981 acgtggacgg gcggtcccag gaaccaggtg tccaaagatt ggttctgacg gcgctgcgtg
     2041 gcgatctcac gccaaagcca gaggcttgcg gccgcaacca gggccagcac cagcgcctcg
     2101 gcgaccgccg gcatgtttcc aaacgccgca acatgccagg gccctgtaac cggcgccagc
     2161 cagtaaagga tgaccgccag ccccgccagc agcgtcgagg cgtaggcgta gagctggctg
     2221 cggcaggcga ggccgagagc ggtggcgatg gcgaagctgg cgagcgtggc ggccagcgac
     2281 caccagggtt gctgcggatc ggaggcgttg ccgcggatag cgaggagggt gatgaggccg
     2341 gcgatggcgg cggcagtaaa gtggcggggg aggagattgt cgccgatcgc tccgcgatcg
     2401 gcacgttctt tcgcggagcg aaagacgaca ctgctgcacg ccgccgcgcc gacgagcatc
     2461 cagccggcgg tgagcgtgtg ataggcgatc cattggccgc cggggtcgta gcggctggcg
     2521 gaggcggcga tgagggcggc cagtccgatg aggaatgcgg cgatcagatc gccggcgtag
     2581 tcgaggctga gcttcgtctc gcgcccccag gcgacccaga tcagcaccgc caggacgatg
     2641 ccgacggaca ggtagctgag caggttgccc agcgtggcga tctcgggcgg cgggttgcct
     2701 ggctcgctga agatcgccca cgcggcccac agggcgaggg ccgccgcggt cgctccggct
     2761 gcggcgatct ggatgtcgag caaaatgcgg gcaacgagct tggacgca
//
LOCUS       DASUGB010000890         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_933795, whole genome shotgun sequence.
ACCESSION   DASUGB010000890 DASUGB010000000
VERSION     DASUGB010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933795"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFV87_16970"
     CDS             complement(<1..761)
                     /locus_tag="VFV87_16970"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5105515.1"
                     /translation="MKSTLLVLGLIAGPLIAGLIPTIQAAGPERPNIVFIFGDDCGID
                     SFGLYGSDRAKALTPHIDALAKTGTRFDRCFATPLCGTSRCLIMTGRHGFRTGGLTNQ
                     TAKNPSFRDEPALAKILKSAGYATGMAGKWRQMSDSPGDWGFDEFITDPTAAGWYWKQ
                     SYTKNGQEVATDKEIYYPDAASDFALDFIRRHRGGPFFFYLAEHLVHGPIVRTPDSKE
                     GAQPHQLYDDNIAYLDKTVGKVVAELDKLGLREKTV"
     gene            962..2203
                     /locus_tag="VFV87_16975"
     CDS             962..2203
                     /locus_tag="VFV87_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105516.1"
                     /translation="MARFEPETSALASAGAPPSAPRKFRSPRPFTLAVIACALTAAFL
                     ANFPAIETPHAGLAEETYGRHFGIVGQLEHGWPLRYVRRSPPAHGATDEPLSPWRPWQ
                     EVQSLSLASLALDLVLWTAIVVAIAAATHYWRSRRWAIWQLDLRDLLLLTGALAAAFA
                     WLAATRADRLREQTLLAEFRQRNGNPQMQHEVATSVPAWWPDTLQGHWRQAFDRTSYY
                     RSSGDTDLASQHRHVLTLRETAFHRDFPKHLRQMPGLEALDLSYTRLPYFDATGQATI
                     LRDLAPLANLRGINLYGTNATDADMAWLAACRNLEMIDLSGTRIGDHGLKQLAALPRL
                     RTLVIDSNRISDDGCRSIARLGSLEELSLASRNIHDQGALALASLTGLRRLKISAAAG
                     DAAFAALREQLPRCEVKAHAY"
BASE COUNT          429 a          820 c          806 g          418 t
ORIGIN      
        1 accgtcttct cgcggaggcc cagcttgtcg agctccgcca ctaccttgcc caccgtcttg
       61 tcgaggtagg cgatgttgtc gtcgtacagt tggtgcggct gggcgccctc cttcgaatcg
      121 ggcgtccgca cgatcggccc gtgaaccagg tgctcggcca gataaaagaa gaacggcccg
      181 ccgcggtggc ggcggataaa atctagggcg aaatcggacg ccgcgtcggg atagtaaatc
      241 tccttgtcgg tcgcgacttc ctggccattt ttggtgtagc tctgcttcca gtaccagccg
      301 gcggcagtcg ggtcggtaat gaattcgtcg aagccccagt cgccgggcga gtcggacatt
      361 tgccgccact tgcctgccat gccggtggca tagccggcgg atttcaaaat tttggccagc
      421 gccggctcgt cccggaacga cgggttcttg gccgtctggt tggtcaggcc gccggtgcga
      481 aagccgtggc gtccggtcat gatcaggcag cgcgacgtac cgcacagcgg ggtggcaaag
      541 cagcggtcga accgcgtgcc cgttttggca agggcgtcga tatggggcgt aagcgccttg
      601 gcccggtccg agccgtagag gccaaaactg tcgatgccgc agtcgtcacc aaagatgaac
      661 acgatgttgg ggcgctcggg ccccgcggcc tggatggttg gaatgaggcc tgcgatcagt
      721 gggccagcga tcaaacccaa gacgagcagc gtcgatttca tgattgtgat tcctgcaaag
      781 agaagcgatg ccctcgtgcc ccagcaatgt aacaagcgga aggacgagcg tgcgatgcgc
      841 ggccgcacgg gattattgtc aagcgcaggg tgaatttcat ccaaattgtc gccaaccggt
      901 tgccgcccct ctgatcgacc gggtcggacc acagcctggc ggaaaaaaaa tggcgggcca
      961 aatggccagg tttgaacctg aaacgagcgc cctagcatcc gccggcgcgc cgccgagcgc
     1021 ccctcgaaag ttccgctcgc cgcggccgtt tacgctggcc gtgattgcct gcgccctgac
     1081 ggctgcattt ctcgcgaatt ttccggccat cgaaacgccg cacgcgggcc tggcggagga
     1141 aacgtacggg cggcatttcg gcatcgtcgg ccaactcgag cacgggtggc cgctccgcta
     1201 tgtgcggcga tcgccgccag cgcatggtgc aaccgacgag ccgctttcgc cgtggcggcc
     1261 ctggcaggag gtgcagtcgc tgagcctcgc cagcctggcg ctcgacctgg tcttgtggac
     1321 cgcgatcgtc gtcgccatcg ccgccgccac ccactactgg cgatcgcggc ggtgggcgat
     1381 ctggcaactg gacctgcgcg atctgctctt gctgaccggc gcactggccg ccgcatttgc
     1441 ctggctggcg gcgacccggg ccgaccgcct gcgtgagcaa accctgctgg ccgaattccg
     1501 ccagcgcaac ggcaaccccc agatgcagca cgaagttgca accagcgtcc ccgcctggtg
     1561 gcccgacacg cttcagggcc attggcgcca ggctttcgac cggacgagct actaccgatc
     1621 cagcggcgac accgacctgg cgagccagca tcgccatgtg ctcacgctgc gagaaaccgc
     1681 gttccatcgc gactttccca agcacctgcg gcaaatgccc ggcctggaag cgctcgacct
     1741 gagctacacg cggctgccgt atttcgacgc caccggccag gcgacgattc tccgcgacct
     1801 cgcgccgctg gccaacctgc gcggcatcaa cctctacggc acgaatgcca ccgacgccga
     1861 catggcctgg ctggccgcct gccggaacct ggaaatgatc gacctttcgg gcacgcggat
     1921 cggcgatcac ggcctgaaac aactggccgc tttgccccgc ctgcggacgc tggtcatcga
     1981 ttcgaaccgc atctcagacg acggctgccg ctcgatcgcc cggctcggct cgctggaaga
     2041 actgtcgctg gccagccgca atatccatga ccagggggcg ctggccctgg cgagcctgac
     2101 cgggctgcgg agattgaaga tcagcgccgc ggccggcgat gcggccttcg ccgctctgcg
     2161 agaacagctg ccgcggtgcg aggtgaaggc ccacgcctat tgacggcggg cgggcgggcg
     2221 ggaatcatcg gcccaggtaa gggtggctgg ggttgaacgg agttcaaccc cggcattccg
     2281 gggtctggct tcgctcgacc ccagccaccc cccgcatcct gcatttcacg aaagaggcga
     2341 ctcatggcgt tttcgcttcg agggcacgtg gcggtcgtca ccggcaacag caccggcctg
     2401 gggaaggcaa tcggcctggc cttgggccgg gccggggcca aggtgccggt caacttcgcc
     2461 cacaacgagg acc
//
LOCUS       DASUGB010000891         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_377582, whole genome shotgun sequence.
ACCESSION   DASUGB010000891 DASUGB010000000
VERSION     DASUGB010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377582"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="VFV87_16980"
     CDS             complement(<1..1699)
                     /locus_tag="VFV87_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5105517.1"
                     /translation="MKLNQPLCVLCWILSLPLALAFMALMAQGADTPAEKLPEGLKVV
                     AIEVSPAAVELKHKFDYRQLLISGKLETGETVDLTRIAQPSSQGAAASISNDGLVRAN
                     ADGSEEIAFTYNSLSAKVAVTVSGITQPHTVSFVRDVQPALSRMGCNAGTCHGAKEGK
                     AGFKLSLRGYDALYDHRAFTDDIGGRRFNRSAPDQSLMLLKATGSIPHVGGVRTNVGD
                     PYYDLVRDWITQGVKLDLNATRVAKIEVAPVNPLVPRPGMKQQVTVMATYTDGSVRDV
                     TREAFIESGNIEIIEANTTGVLTTLRRGEAPVLVRYEGAYAATTIIVMGDRTGFAWEQ
                     RPQYNYIDQHVDHKLQFVKIVPSELCSDGEFVRRVYIDLTGLPPTSQQVREFLDDPTE
                     SRAKRDALVDRLVGSREYVEHWTNKWADLLQVNRKFLGEPGAKALRGWIKNAVATNMP
                     YDEFAHAVLTASGSNVANPPASYYKVLRTPDEVMENTTQLFLAIRFNCNKCHDHPFER
                     WTQDQYYEMAAFFAQVSRTEDPKYKGQKVGGTAVEKPLPLVEVIADGKFGEVKHDRTG
                     "
     gene            complement(1802..3217)
                     /locus_tag="VFV87_16985"
     CDS             complement(1802..3217)
                     /locus_tag="VFV87_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX5105518.1"
                     /translation="MLLRTFAGASLLTMVAAGPAWAEEPAPAEGPVSYFKQVLPIFRT
                     KNCTGCHQQAKKGGEYVMTEFADLVKGGETGEPAIVPGDPAKSNLIAQITPSNGKAEM
                     PKDAPPLTEAEIATITRWIEQGAKDDTPASNKPQYDAEHPPMYMAPPVINSIEYSPKG
                     DLLAIAGYHEVLLHNADGSGLVARLVGQAERIESARFSPDGKFLAVTGGSPGRFGELQ
                     IWDVEKREMTLSLMIGYDTCYGASWAPSGKLVAVGCPDNTVRAFDVETGKQVLFSGAH
                     SDWVLDTSFSVNSDHLVSVSRDMSMKVVEVATQRFIDNVTSITPGALKGGLMAVERHP
                     TKDEFLAGGSDGQPKIFRMLRTSARQIGDNANLIREFGALPGRVFGVAFSPGGDRIAA
                     GSSSNGTGEVRVYNAADGAVVWQMKVPEGGIFSVDFSPDGNTVAATGFDGDVRLYSAA
                     DGKLIKQFTPVQVTGPAVAAN"
     gene            3466..>3885
                     /locus_tag="VFV87_16990"
     CDS             3466..>3885
                     /locus_tag="VFV87_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105519.1"
                     /translation="MPVMTAADESPLAGAGPAIDWARSLSEHEAWLRRVILARTSEPQ
                     AVEEVWQQVALAAIEQRWPLTDPAKVAPWLHRLAVIAAARYCRSAGRRRRATATLASL
                     AAANGSPAASDPLVLLMRRERLELTRQAMARLAPRDAE"
BASE COUNT          614 a         1208 c         1320 g          743 t
ORIGIN      
        1 ccccggtgcg gtcgtgcttg acctcgccga acttcccgtc cgcgatcacc tccacgagtg
       61 gcagcggctt ctcgactgcg gtgccgccga ccttctggcc cttgtacttc gggtcctcgg
      121 tccggctgac ctgggcgaag aacgccgcca tctcgtagta ctggtcctgg gtccaccgct
      181 cgaacgggtg gtcgtggcac ttgttgcagt tgaaccggat cgccaggaac agttgggtgg
      241 tgttctccat cacctcgtcc ggcgtccgca gcaccttgta gtacgacgct ggcgggttgg
      301 cgacgttcga cccggacgcg gtcaggaccg cgtgggcgaa ctcgtcgtaa ggcatgttgg
      361 tggcgacggc gttcttgatc cacccgcgca gcgccttcgc ccccggctcg ccgaggaact
      421 tgcggttcac ctgcaacagg tccgcccact tgttcgtcca gtgctcgaca tactcgcggc
      481 tgccgacgag gcgatcgacg agggcatcgc gcttggcgcg gctttcggtg ggatcgtcca
      541 ggaactcgcg gacctgttgg ctggtgggcg gcaggccggt gaggtcgata tagacgcggc
      601 ggacaaactc cccgtcgctg cacaactcgc tcggcacgat cttgacgaac tgtagcttgt
      661 gatcgacgtg ctgatcgatg tagttgtatt gcggccgctg ctcccaggcg aagccggtgc
      721 gatcgcccat cacgatgatc gtggtggcgg cataggcccc ttcgtagcgg acgagcacgg
      781 gggcttcgcc gcggcggagc gtggtgagga cgccggtggt gttcgcctcg atgatctcga
      841 tgttgccgct ttcgatgaaa gcttcgcggg tgacatcgcg gaccgagccg tcggtatagg
      901 tcgccatgac ggtgacctgc tgcttcatgc cggggcgggg cacgagggga ttgaccggcg
      961 cgacttcgat cttggcgacg cgcgtggcgt tcaggtcgag cttgaccccc tgcgtaatcc
     1021 aatcgcggac caggtcgtag tagggatcgc cgacgttcgt tcgcacgcca cccacgtgtg
     1081 ggatcgagcc ggtcgccttg agcagcatca agctttgatc gggcgccgaa cggttgaacc
     1141 gccggccgcc aatgtcatcg gtgaaggccc gatgatcgta tagggcgtca tagccccgca
     1201 gcgacagctt gaagcccgct ttgccttcct tggccccgtg gcacgtgccg gcgttgcagc
     1261 ccatgcgcga gagggccggc tgtacgtcgc ggacgaagct gaccgtgtgc ggctgcgtga
     1321 tgccagagac cgtgacggcc acctttgccg agaggctgtt gtacgtaaag gcaatctcct
     1381 cgctgccgtc ggcgttggcg cggaccaggc catcgttgga aatgctcgcc gcggcgcctt
     1441 ggctcgaagg ctgggcgatg cgcgtcagat cgaccgtttc gcccgtctcg agcttgccgc
     1501 tgatcaggag ttggcggtaa tcgaacttgt gctttaattc gaccgcggcc gggctgactt
     1561 cgatcgccac gaccttcagc ccctccggca gcttctccgc cggggtatct gcgccctgtg
     1621 ccatcagggc catgaacgcc agtgccagcg gcaaggaaag aatccagcag aggacgcaga
     1681 gaggttggtt cagcttcatc agactcttcc atttagccgg gaccaaacct ggtcccgggg
     1741 cgctgcaagt tgactgtgat ttgggactcg ccgggaccag gtttggtccc ggctaaggaa
     1801 gttaattggc cgcgaccgcg gggccggtga cttggacggg agtgaattgc ttgatcagct
     1861 tgccgtcggc tgcggaatac agccgcacgt cgccatcgaa gccggtggcg gcgacggtgt
     1921 tgccatcggg gctgaaatcg accgagaaaa tgcccccttc gggcaccttc atctgccaga
     1981 cgacggcacc gtcggcagcg ttatagacac ggacttcgcc cgtgccgttg ctggagctgc
     2041 cggcggcgat gcggtcgccg cccgggctga aggcgacgcc gaacacgcgg ccgggcaaag
     2101 cgccaaattc gcgaatcagg ttcgcgttgt cgccgatctg ccgggccgac gtgcggagca
     2161 tccggaagat tttcggctga ccgtcgctgc cgccggcgag gaattcatcc ttcgtcggat
     2221 gccgctcaac ggccatgagg ccccccttca gcgcgccggg cgtgatgctg gtcacgttgt
     2281 cgatgaatcg ctgcgtcgcg acttccacga ccttcatcga catgtcgcgg ctgacgctga
     2341 cgaggtggtc ggaattgacc gagaagctgg tgtcgagcac ccagtcgcta tgagccccgc
     2401 tgaacaggac ctgtttgccc gtttcgacat cgaatgcccg caccgtgttg tccgggcagc
     2461 cgacggcgac cagcttgccg ctgggggccc agctcgcgcc gtagcaggtg tcgtagccga
     2521 tcatcagcga gagcgtcatc tcgcgttttt cgacatccca gatctgcaat tcgccgaagc
     2581 ggccgggcga gccgcccgtg accgccagga acttgccgtc gggcgagaag cgggccgatt
     2641 cgatccgctc tgcctggccg acgagccggg cgacgaggcc gctgccgtcg gcattgtgca
     2701 ggagcacttc gtgatagccg gcgatggcca gcaagtctcc cttgggtgag tactcgatcg
     2761 aattgatcac cggcggggcc atgtacattg gcggatgctc ggcgtcgtat tgcggcttgt
     2821 tgctggccgg tgtgtcgtcc ttggcgcctt gttcgatcca cctggtgatc gtggcgatct
     2881 cggcttcggt cagcggcggc gcgtccttgg gcatttccgc cttgccattg gaaggcgtga
     2941 tctgggcaat gaggttgctt ttggcgggat cgcccggcac gattgccggt tcgcccgtct
     3001 cgcccccttt gacgaggtcg gcgaactccg tcattacgta ttcgcccccc ttcttcgcct
     3061 gctggtggca gccggtgcag ttcttggtgc ggaagatcgg caggacctgc ttgaaatagc
     3121 tgaccggacc ctcggccggg gcgggctctt cggcccaggc gggcccagcg gcaaccatcg
     3181 tcagcaatga agcaccagcg aacgtgcgga gaagcatgtc gcgttccttg gggagcgata
     3241 acgctccgcg tgaatcgaag caccttgcca gagcaagacg cctgcgtcgt cgaggcggga
     3301 aaaaccaacg gcgggctcag cctcgaatat agccgcaggc cccctgcatt gcaatcattg
     3361 agcggccaaa atgaaggggc taatcccttg cgctgattga ggttaggaat gccttgccgt
     3421 gatagccgat cgggctgagg gattttcgat cagggaggaa cttctttgcc agtgatgacc
     3481 gctgctgacg aatcgccgct tgcgggtgcc ggaccagcga ttgactgggc gcgcagcctg
     3541 agcgaacacg aggcgtggct gcggcgggtc attttggccc gcaccagcga gcctcaggcg
     3601 gtcgaggagg tgtggcagca ggtggccctg gcggcgatcg agcagcgctg gccgctgacg
     3661 gacccggcca aggtcgctcc ctggctgcac cgcctggcgg tcatcgccgc cgcccgctac
     3721 tgccgctccg ccggccggcg aaggcgggcg accgcaacgc tggcgtcact ggcggcggcg
     3781 aacggttcgc cagccgcaag cgatccgctg gtgctgctga tgcggcgcga gcggctggag
     3841 ctgacgcggc aggcgatggc tcgcctggcc ccgcgggacg ccgag
//
LOCUS       DASUGB010000892         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_517121, whole genome shotgun sequence.
ACCESSION   DASUGB010000892 DASUGB010000000
VERSION     DASUGB010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517121"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..967)
                     /locus_tag="VFV87_16995"
     CDS             complement(137..967)
                     /locus_tag="VFV87_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105520.1"
                     /translation="MRTLPRWSIWLLWSLSIVAASIVAAVLAHETGTNHPALFGIGFG
                     ALCAIGTIHAIWTVFGPLALITRVPLALLIQSAVVVAVAAVEGGVHYGIEFVGLFGGF
                     LFGQYFLAQLPLWFIALRWRARIASGTMQPGTLAQPDLQFQIRHVMILTAGVAIALGA
                     ARGLIQIGDLRTSWPEMQLVRVIAFLVLCNTTIVLPIAVAALIRRRALLASAVAWTLV
                     FLATIVEVPTFLLVRGVGATTHELWFVFWAMNLTQATWVFLSMGILRLGGYRLEIRPP
                     "
     gene            complement(970..1866)
                     /locus_tag="VFV87_17000"
     CDS             complement(970..1866)
                     /locus_tag="VFV87_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5105521.1"
                     /translation="MPLSRRSLLAGSAAAASAALIAAPPTAGEEQDSAKIASLGKTKN
                     TKFAVNCEMWWNKLPFLERLRKAAEFGFSAVEFWPYQGKDIAAVKKLKDELQLDIAQF
                     TAWGFEPGMNDPKQEDSFVGRIEEACQVAHQLDCQKMCVVAGNNQKGMTKDQMHAQVI
                     KALKRAAPVAEREKVMLILEPMNGRVDHPGHCLYGSEDAVKICRAVDSPMVKINWDLY
                     HMQIAEGDLCGRLKEGFDQLGYLQLADHPGRNEPGTGEIYYSRVLRQAKELGYTGYVG
                     LELRPKTTELNAAMAVNKADEW"
     gene            2039..>2945
                     /locus_tag="VFV87_17005"
     CDS             2039..>2945
                     /locus_tag="VFV87_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105522.1"
                     /translation="MIVSGRFFAGLAAAAVLTAAGCLPPVPGGNLNLPAPATPLESAW
                     VELIEVQEAQFQKLASIHNESQAEAAISGAIEYQKKRHAALKKIAELGGQKPPTGVPE
                     KYREEYKAVEDRVYARDEVVSQMISPEKREQLNQQLRQALQSDPELAQDPFALSAEIR
                     AAGAGNRATVTLVNNQSLQGSAHQQMIARLQQLAGASNAESVIENDGTYNLVLAPVTD
                     FGGFVAKIDFGDISNRDDSAKTFTLTIEPQRFQAVAGNPADPADPRTAGPPGFGRPGG
                     AAPASGGPGRPPFGAPAAGQNDAERQ"
BASE COUNT          588 a          922 c          903 g          532 t
ORIGIN      
        1 cagccacgac aagccgagca gcagcccaag cgggacgagg acggcgtagc gaccgcgttg
       61 catgtgtaat cctcccagca gtttcgctga ccactgatct tcgtcgccag ttgcacgaag
      121 tgcttgttgt ggttctctaa ggcggtcgaa tctccaggcg atagccgccc agtctcagga
      181 ttcccatgga taggaagacc caagtcgcct gcgtcagatt catcgcccaa aagacgaacc
      241 agagttcgtg ggtcgttgcg cctacgccgc gcaccaaaag aaatgtcgga acctcgacga
      301 tcgtcgccag gaaaacaaga gtccacgcca cggctgatgc taatagggcg cgcctacgaa
      361 tcagcgctgc cacggcaatg ggcagcacga tggtggtatt acacaagacc aggaaggcga
      421 tgacccggac caactgcatt tctggccacg acgtgcgcag gtccccgatc tggatcaagc
      481 ctcgtgccgc tcccagcgcg attgctacgc cagccgtcag gatcatcacg tggcgaattt
      541 gaaactgcag gtccggctgg gcaagtgtgc ccggttgcat cgtcccgctc gcgattcgcg
      601 cacgccacct tagtgcgatg aaccaaagcg gaagttgcgc aagaaagtat tgaccgaaca
      661 gaaaaccacc gaacaggcca acgaattcga tcccatagtg aacgcccccc tcgactgccg
      721 cgacggcaac aacgacagcc gattgaatca gcaacgcgag cggcactctc gtgattaacg
      781 ccaacggacc aaataccgtc cagatcgcgt ggatggtgcc gatggcacac aatgcgccga
      841 atccaatacc gaataacgcg ggatgattgg tccccgtctc gtgggccaac accgccgcca
      901 caatcgacgc cgccacgatc gatagcgacc aaagcaacca gattgaccag cgtgggaggg
      961 tccgcatcgc taccactcat ccgccttgtt caccgccatc gcggcattca gctcggtcgt
     1021 cttcggccgc agctccagac cgacgtagcc ggtgtagccc agctccttcg cctgacgcag
     1081 gacgcggctg tagtagatct cgccggtgcc gggctcgttg cggccgggat ggtcggcaag
     1141 ctgcaggtag ccgagctggt cgaagccctc cttcagccgg ccgcagaggt cgccttcggc
     1201 gatctgcatg tggtagaggt cccagttgat cttgaccatc gggctatcga cggcccggca
     1261 gattttgaca gcgtcctcac tgccgtagag gcagtggccg ggatgatcga cgcggccgtt
     1321 catcggctcg aggatcagca tgaccttctc tcgctcggca acgggggcgg cgcgcttcag
     1381 ggccttgatg acctgggcgt gcatctggtc tttcgtcatc cccttctggt tattgccggc
     1441 gacgacgcac atcttttggc aatcgagctg gtgggccacc tgacaggcct cctcgatgcg
     1501 gcccacgaac gagtcctcct gtttcgggtc gttcatgccc ggctcgaagc cccaggccgt
     1561 gaactgagcg atgtcgagtt gcagctcgtc cttgagcttc ttcaccgccg cgatgtcctt
     1621 cccctgataa ggccagaatt cgaccgccga aaagccgaac tccgccgcct ttcgcaggcg
     1681 ttcgagaaac ggcagcttgt tccaccacat ttcgcagttg acggcgaact tcgtgttttt
     1741 ggtcttgccg agcgatgcga ttttggcact gtcttgttcc tcgcctgcag tcggcggagc
     1801 agcaatgagt gcggccgaag cggcagcagc agaaccggca agaagcgaac ggcgggagag
     1861 aggcatggtg gtgctcctgg atttcgaaga gtcgagcgag tgcgattata gcctgccttg
     1921 gcgcgcaggc gggttgcgaa acgccaccgc gtttcgtacg ttcgcatgag gcgctttatc
     1981 ccgtctgaga ttactcaaat tcgtgagggg tgtgatggtg ggttcgttgc aacactgtgt
     2041 gatcgtttcc ggtcgctttt tcgctggcct ggcggccgcc gccgtgctga cggctgctgg
     2101 ctgcttgccg ccggtgccgg gaggcaattt gaatctgccg gcgccggcca cgccgctgga
     2161 aagcgcctgg gtcgagctga tcgaagtgca ggaggcccag ttccaaaagc ttgcgtcgat
     2221 ccacaacgag tcgcaggcgg aagcagccat cagcggggct atcgagtatc agaaaaagcg
     2281 ccacgcggct ctcaagaaga tcgccgagct tggcggccag aagccgccga ccggtgtgcc
     2341 ggagaagtat cgcgaagagt acaaggcggt cgaggacaga gtgtatgccc gcgacgaggt
     2401 cgtctcgcag atgatcagcc ccgaaaagcg cgagcagctc aatcagcagc tccggcaggc
     2461 gctgcaatcc gatccggagc tggcccagga cccgttcgcc ctctcggcgg aaatccgcgc
     2521 tgccggggca ggtaacaggg ccacggtgac cctcgtcaac aaccagtcgc tccaaggctc
     2581 ggcccaccag caaatgatcg cccggctgca gcaactggct ggagcgagca acgccgagtc
     2641 ggtgatcgag aacgacggca cgtacaacct tgtgctcgcg ccggtaaccg attttggtgg
     2701 cttcgtggcc aagatcgact tcggcgacat ttcgaatcgc gacgattccg ccaagacctt
     2761 caccctgacg atcgaaccgc agcggttcca ggcagtggcc gggaatcccg cggatccggc
     2821 tgatccacgc accgccggtc cgccgggctt tggcagaccc ggcggcgctg ctccagcctc
     2881 cggcggtccg ggccggccgc cgtttggcgc tccggcggct ggccagaatg acgccgagcg
     2941 acaaa
//
LOCUS       DASUGB010000893         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_933893, whole genome shotgun sequence.
ACCESSION   DASUGB010000893 DASUGB010000000
VERSION     DASUGB010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933893"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1535)
                     /locus_tag="VFV87_17010"
     CDS             complement(108..1535)
                     /locus_tag="VFV87_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5105523.1"
                     /translation="MAEERENDAAEPAVPPIRLLVVDNDEALARAMEESLAKVGYDCV
                     VATSGPEGARRIDTDSFDIVVTDLVMNDVDGMEILARSREKLPDAEVILVTGHATVPK
                     AVEAMQQGAFNFLEKPITTTRLRAVCEKAADAIRLKRQNVELRERLDERFGFEGIIYS
                     SSPMQAVIDRLKRIAPTDATVLIHGESGTGKELIAQAIHQNSPRKGKRIVAINCAAVA
                     ENLVESELFGHVKGAFTDAHADRIGAFEFANGGTLFLDEVGDMPMSTQIKLLRVLEEQ
                     TITRVGDNKSIRVNVRVVSATNRPLEDLVAAGKFRNDLYFRLKVVTVNLPPLRERRED
                     IVPLADHFRRQFAKRHHKQVKGMSPSATKRLFAYEWPGNVRQLRNAVETMVVLDVDGT
                     LDTDDLPPELSEIAEPSGPVAVSGGPMELIGRTMDDIERWAIEETLKLTNGNREEAAK
                     ILGIGARTLYRKLEKYAGGEGEREG"
     gene            complement(1644..2381)
                     /locus_tag="VFV87_17015"
     CDS             complement(1644..2381)
                     /locus_tag="VFV87_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5105524.1"
                     /translation="MTDHQPLSTEQALEQLRERYAELAALAGSLAHEIKNPLSVIRMN
                     MDLLGEDFAQPETPRERRALAKIEMVGRQCTRLENLLNDFLRFNKVSQLDLHVGSLNE
                     QITRVLDLFEPQAQEINVEIVRYLDPDLPSIQLNAETLQASLVNLVKNALEAMPDGGQ
                     LIARTRVTRHGVALDLIDTGVGMDERTAMKMFDAFFSTKSGGSGLGLPTARRIIEAHG
                     GRIGVHSEAGRGTQFTLEFPTPARIGE"
     gene            2604..3065
                     /locus_tag="VFV87_17020"
     CDS             2604..3065
                     /locus_tag="VFV87_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105525.1"
                     /translation="MAKPIARRQLFVNGPIQGALVTRVAVYWLLCMFVQVLLLMFVSV
                     GSLGSDGLFQPTPQIGWQLKLMAFSTLLTLPLLTYDIIKLSHRWVGPIYRLQAAMHAL
                     AMGEAVEPVVFRAGDYWKDLGDDFNAVLTRVEHLQRNYEPAECEEAGPVAG"
     gene            complement(3101..5446)
                     /gene="pnp"
                     /locus_tag="VFV87_17025"
     CDS             complement(3101..5446)
                     /gene="pnp"
                     /locus_tag="VFV87_17025"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5105526.1"
                     /translation="MKVRVEKQIGASILSFETGLLAKQAHSAVIAQYGESVVINAVTT
                     GSPRPGIDFFPLTCDYRERTAAAGKFPGGFIKREGRPTTKETLTSRLCDRPIRPLFPK
                     GFKDEVQCQMIVLASDKQNDADVIAMNGIAAALFVSPLPFSGPIASVRVGRVGGQFVA
                     FPTHDDLEDSDLDLIMSGTQESVAMIEGFSREMSENDMFEAVLFGHGIIREIIALQRE
                     LYEKIGVQKTPFEAPADTGLYDRLKRSYYDAFKTAKSTSGKQNRAEAVKALKEKALAE
                     VIPDPLAADAIKPEVFSSAWHDLEERVVRDQILSGTRPDGRDYRSLRNIDCLVDVLPR
                     VHGSAMFQRGETQALVTVTLGTSRDEQRVDGLFDEYSKKFMLDYNFPPFSVGETKPIR
                     GPGRREIGHGALAERSVNPVLPSPEEFPYTIRVISDILESNGSSSMASVCGATLGLMA
                     AGVPISNPVAGISVGLVKESETQWVLLTDIIGDEDHYGDMDFKIAGTQNGITGIQLDL
                     KIEGISPEIIRATFDQSREARIQILRKMLTAISRPKEDISPWAPRLIRTQIDPEKIGL
                     LIGPGGKNIRGIQEATGTVIEVEDDGTVTIASSNREWAEAALAQVEACTATVQIGKIY
                     TGRVTSIKDFGAFVEILPGRDGLVHVSELSSGYVSAVHDVCNIGDEMKVLVIDVDEHD
                     RVKLSRRRAYEELGLPDEMAAVPAGERPPRGEGEGDYGGGDRGGDRGDRGGYRGGDRG
                     GRGGGRGGDRGGRGGGRGGRGGGGGRGYGDRGGGGGYGDRR"
BASE COUNT          867 a         1827 c         1795 g         1096 t
ORIGIN      
        1 gggaggcacg tcaaagcccg caggcagaat ggagttggtg aagccgatgc tcatggttgg
       61 aaaccaccaa gatacctcat tctaacatcg ctggacaaag ccgcaattca cccctccctt
      121 tcgccttcgc cgccggcata cttctccagc ttccgataca gcgtccgcgc tccaataccc
      181 aggatcttgg cggcttcttc gcggttgccg ttggtgagct tgagcgtctc ttcgatggcc
      241 cagcgctcga tgtcgtccat cgtgcgtccg atcagctcca tcggcccgcc gctgacggcg
      301 acggggccgc tcggctcggc aatttcggac agctccggcg gcagatcgtc cgtatcgagc
      361 gtgccatcga cgtcgagcac gaccatcgtt tcgaccgcgt tgcgaagctg gcggacgttg
      421 ccgggccatt cgtaggcgaa cagccgcttc gtggccgagg gagacatccc tttgacttgc
      481 ttgtggtgcc gcttggcgaa ctggcggcgg aagtggtcgg ccagcggcac gatgtcctcg
      541 cgccgctcgc ggagcggcgg cagattgacc gtgaccacct tcaggcggaa gtacagatcg
      601 ttgcggaact tgcccgctgc caccaggtct tccagcgggc ggttggtcgc ggacaccacc
      661 cgcacgttga cgcggatcga cttgttgtcg cccacgcggg taatcgtctg ctcttccagc
      721 acgcgcagca gcttgatctg cgtgctcatt ggcatgtcgc cgacttcgtc cagaaagagc
      781 gtgccgccgt tggcgaactc gaacgcgccg atccggtcgg cgtgggcgtc ggtgaaggcg
      841 cccttcacgt ggccgaacag ctcgctttcg accaggtttt cggccaccgc ggcgcagttg
      901 atggccacga tccgctttcc cttccgcggg ctgttctggt ggatcgcctg ggcgatcagc
      961 tccttgcccg tgccgctttc gccgtggatc aggacggtcg cgtcggtcgg ggcgatccgc
     1021 ttcagccggt caatgaccgc ctgcatcggg ctgctggagt agatgatccc ctcgaaaccg
     1081 aaccgctcat ccagccgctc ccgcagttcg acgttctgcc gcttgaggcg gatcgcgtcg
     1141 gccgcctttt cgcagacggc ccgcaggcgc gtcgtcgtga tcggcttttc cagaaaattg
     1201 aacgccccct gctgcatcgc ctcgacggct ttggggacgg ttgcgtggcc ggtgacgagg
     1261 atcacttcgg cgtccggcag cttttcgcgt gagcgggcca ggatctccat cccatcgacg
     1321 tcgttcatca ccaggtcggt gacgacgatg tcgaacgaat cggtatcaat ccgccgcgcc
     1381 ccttccggcc cgctggtcgc cacgacgcag tcgtagccca ccttcgccag gctctcttcc
     1441 atcgcccggg ccagagcctc atcgttgtcg acgaccagca gtcgaatcgg cggcacagcc
     1501 ggctcggcag cgtcgttttc acgttcctca gccatgcagg cgatgatagc gaagggcctc
     1561 gcgagcggcg agaggcggtg ctatctccgc cgcccacctg tgcatctcat tccgcaatcc
     1621 gcattcagaa ctccgcactc gattcactct ccaatccgcg ccggcgtggg gaattcaagc
     1681 gtgaattgcg tgccgcggcc ggcttcagag tggacgccga tgcggccgcc gtgggcttcg
     1741 atgatccggc gggcggtggg cagccccagg ccgctgccgc cgcttttggt gctgaagaag
     1801 gcgtcgaaca ttttcatcgc ggtccgctcg tccataccga cgcccgtgtc gatcaggtcc
     1861 agggccacgc cgtgccgcgt cacccgcgtg cgggcgatga gctggccgcc gtcgggcatc
     1921 gcctccaggg cgttcttcac caggttgacg agcgacgcct gcagcgtctc agcattgagc
     1981 tggatgctcg gcaggtcggg atcgaggtag cggacaatct cgacgtttat ctcttgggcc
     2041 tgcggctcga agagatcgag gacccgggtg atctgctcgt tcaggctgcc gacgtgcagg
     2101 tcgagctggc tgaccttgtt gaaccgcaga aagtcattga gcaggttctc cagccgcgta
     2161 cactggcggc cgaccatctc gatcttggcc agggcccggc gttcgcgcgg cgtttccggc
     2221 tgggcgaagt cctcgcccag caggtccatg ttcatccgga tgaccgacag cgggtttttg
     2281 atctcgtggg ccagcgagcc ggccagcgcc gccagctccg cgtagcgctc gcggagctgc
     2341 tccagggcct gttcggtcga gagcggctgg tggtcggtca tgcgatttat tgtgacacgg
     2401 acaagcccag gcagggcata cgcacccgcg aggccggacc tctcattgca aaacgaataa
     2461 tccccccagc cgggacgact ggcgcttctg ggcgcagcaa cccctccgac atttcgtggc
     2521 cgaatggact aggcttgtac gggcaattct ggcgatcacg ccgcgagttg tcgctccgcc
     2581 aattcgtccc aggggcagaa ccgatggcca aaccaatcgc gcgacgccag ctcttcgtga
     2641 acggcccgat tcaaggcgcg ctggtcacgc gggtggcagt gtactggctg ctctgcatgt
     2701 tcgtccaagt gctgctgctc atgttcgtct ctgtcgggtc gctgggaagc gacggactct
     2761 ttcagccgac gccgcaaatc gggtggcaac tgaagctcat ggcgttctcc actctattga
     2821 ccctgccgct cctgacatac gacattatca agctcagcca tcgttgggtc gggccgatct
     2881 accggctgca ggccgccatg cacgccttgg ccatgggcga ggccgtcgag cctgtggtct
     2941 ttcgcgcagg cgactactgg aaggacttag gcgacgactt caatgccgtg ttgacgcgtg
     3001 tcgagcacct ccagcgcaat tacgagccgg ccgaatgtga ggaagccggg cccgttgcgg
     3061 gctaacgacg aaccggccag ttcgggcaac gccggcagcc ctaccggcga tcgccgtaac
     3121 cgccgccgcc gccacggtcg ccatagccgc ggccaccgcc gccgccgcgt ccgccacgtc
     3181 cgccgccgcg tccgccgcgg tcgccgccac gtccaccgcc gcgtccgccc cggtcgccac
     3241 cgcggtagcc gccgcgatcg ccgcggtcgc cgccacgatc gccgccgccg taatcgcctt
     3301 cgccttcgcc gcgcggcggc cgttcgccgg ctggcacagc ggccatctcg tcgggaaggc
     3361 cgagctcttc ataagcccgc cgccggctga gcttcacgcg gtcgtgctca tcgacgtcga
     3421 tcaccagcac cttcatctcg tcgccgatgt tgcacacgtc gtgcaccgcg ctcacatagc
     3481 cgctcgaaag ctcgctgacg tgaaccaggc cgtcgcggcc gggcaggatt tcaacgaacg
     3541 cgccgaaatc cttgatgctc gtcacgcggc cggtgtaaat cttgccgatc tgcacggtcg
     3601 ccgtgcaagc ctcgacctgg gccagcgccg cctcggccca ttcgcggttg gagctggcga
     3661 tcgtcaccgt gccgtcgtcc tcgacttcga tcaccgtgcc ggttgcttcc tggatgccgc
     3721 ggatgttctt gccgccgggg ccgatcaaga ggccgatctt ttccggatcg atctgcgtgc
     3781 ggatcagccg cggcgcccac ggcgagatgt cttccttggg ccgcgaaatc gcggtcagca
     3841 ttttccgcag gatctggatg cgggcctcgc gcgactggtc gaacgtggcc cggatgatct
     3901 cggggctgat cccctcgatc ttcaggtcga gctggatgcc ggtgatgccg ttctgcgtgc
     3961 cggcgatctt gaaatccatg tcgccgtagt ggtcttcgtc gccgatgatg tcggtgagca
     4021 ggacccactg cgtttcggat tccttgacca ggccgaccga gatgccggcc accggattgc
     4081 tgatcggcac gccggcggcc atcaggccga gcgtcgcgcc gcacacgctg gccatgctgg
     4141 agctgccgtt ggactcaagg atgtcggaga tgacgcggat cgtgtaaggg aattcttcgg
     4201 ggctgggcag gaccgggttc acgctccgct cggccagggc gccgtggccg atctcccgcc
     4261 ggccggggcc gcggatgggc ttcgtctcgc ctacgctgaa gggcgggaag ttgtagtcga
     4321 gcatgaactt tttcgagtat tcgtcgaaca ggccatccac ccgctgctcg tcgcgcgagg
     4381 tgccgagcgt gacggtcacc agggcctgcg tttcgccgcg ctggaacatc gccgagccgt
     4441 gtacccgcgg caggacatcg accaggcagt cgatgttccg cagcgagcga tagtcgcggc
     4501 cgtcggggcg cgtgccgctg aggatctggt cgcggaccac tcgctcttcc aggtcgtgcc
     4561 aggcggagct gaaaacctcc ggcttgatgg catcggcggc gagcggatcg ggaatgacct
     4621 cggccagggc cttctctttg agcgccttga ccgcttcggc ccggttctgc ttgccggagg
     4681 tcgatttggc ggttttgaat gcgtcgtaat aactgcgctt gaggcggtcg tagagtcccg
     4741 tatcggccgg cgcttcgaag ggcgtttttt ggacgccgat tttttcgtag agctcgcgct
     4801 gcaaggcgat gatctcgcga atgatcccgt ggccgaagag cacggcctcg aacatgtcgt
     4861 tctcgctcat ttcgcgcgag aagccttcga tcatggcgac ggattcctgc gtgcccgaca
     4921 tgatcaggtc caggtcgctg tcttccaggt cgtcgtgcgt gggaaaggcg acaaactgcc
     4981 cgcccacgcg gcccacgcgg accgaggcga tcggccccga gaacggcaag ggcgaaacga
     5041 acagcgccgc ggcgatgccg ttcatggcga tcacgtcggc gtcgttctgc ttgtcgctgg
     5101 ccagcacgat catctggcac tgcacttcgt ctttgaagcc cttgggaaag agggggcgga
     5161 tcggccggtc gcacagtcgt gaggtgaggg tttccttggt cgtcggccgg ccttcgcgct
     5221 tgatgaagcc gccgggaaac ttgcccgccg cggcggtccg ctcgcgatag tcgcaagtga
     5281 gcgggaagaa atcgatcccc ggccgcgggg aaccggtggt cacggcgttg atgaccacgc
     5341 tttcgccata ttgggcaatg accgccgaat gggcctgctt ggcgagaagt cctgtttcga
     5401 acgagaggat gctggccccg atttgctttt cgactcttac tttcacttcg ttttcctgcc
     5461 tttacgttgg gtcccgtaat gcaccgagag gagagatgta ggacaggttt cccaacctgt
     5521 gtgtaggaca ggtttcccaa cccgtccggg acgggttgca atcccggcct gctcggacgg
     5581 gttgg
//
LOCUS       DASUGB010000894         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_933922, whole genome shotgun sequence.
ACCESSION   DASUGB010000894 DASUGB010000000
VERSION     DASUGB010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933922"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFV87_17030"
     CDS             complement(<1..973)
                     /locus_tag="VFV87_17030"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX5105527.1"
                     /translation="MQFSIGGTCSVIIYGMRTTESTLGQGLFACPNCRTQQACRHVVM
                     NRWFTLYFIPVIPLGNIGEQFECQGCYARFTADAPGMTPGMAPALTPGSPTTPQPAAG
                     PAWSGQPYAPQAAGPPTAPFATLSLVLGLISPVLLCACGLSALTSLGAIISGHIALAK
                     IAKSKGVLTGRGMAISGLVLGYVLLAFSILFWVRLGPSIFRAGDRAERGAANADVAQT
                     PEDRLLETELRVLTASTEGVATGNTRAAKEIAGEYAESLKSMRDDLFTKDRERVLSLT
                     DGHFIVHCELHADRCALIVHVPGYRDFTSDAKKTLEELAWILAQKAAQ"
     gene            complement(1042..1899)
                     /gene="metF"
                     /locus_tag="VFV87_17035"
     CDS             complement(1042..1899)
                     /gene="metF"
                     /locus_tag="VFV87_17035"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX5105528.1"
                     /translation="MPFYPARPFTLSFELFPPKTAAGEASLYDNVGHLMAFQPDVITC
                     TFGAGGSTRDKTLEITTQVRRRFGVRVASHLTCVGSTTDQLRGYLREARGAGIENIVA
                     LRGDPPKGETAFKPVEGGLRYASELVELIRREFPSFGIAVAGYPETHQEATSPQADLE
                     NLARKVKAGGEVVITQLFYSNADFFRFRDRCQELGITAPIVPGILPVTNLAQIQRITS
                     LCKACLPADFVAELSQKDDPDWQFQAGVCQATRQVQELIDAGIPGIHFYVLNKSPATA
                     AVLDGIRRP"
BASE COUNT          367 a          626 c          705 g          358 t
ORIGIN      
        1 cttgagcggc cttctgggcc aggatccagg cgagctcttc cagcgtcttc ttggcatcgc
       61 tcgtgaagtc ccgatacccc ggcacatgga cgatcagggc gcagcggtcg gcgtgcagct
      121 cgcagtgaac gatgaagtgt ccgtcggtca acgacaggac ccgctcccga tctttcgtga
      181 acaggtcgtc ccgcatcgac ttgagcgact cggcatattc ccccgcgatc tcctttgcgg
      241 cccgcgtgtt gccagtggcc acgccttcgg tgctggccgt cagcacgcgc agctccgtct
      301 ccagcagccg gtcttcgggc gtctgggcga cgtccgcatt cgccgcgcct cgctccgcgc
      361 gatcgccggc gcggaaaatc gacggaccca ggcggaccca aaagagaatc gagaaggcga
      421 gcaggacgta gcccagcacc aggccggaaa tggccatccc gcggcccgtc agcacgccct
      481 tggacttcgc aatcttggcc agagcgatat gcccgctgat gatcgcaccg agggaagtca
      541 gcgccgacag cccgcacgcg cagagcagca cgggcgaaat gagccccagc accaggctca
      601 gcgtggcaaa cggcgcggtg ggagggcccg cggcctgcgg cgcatacggc tgcccggacc
      661 aggcaggacc ggctgcaggt tggggggtcg tgggactgcc cggcgtcaag gcaggcgcca
      721 tgccaggcgt catgcccggc gcatcagccg tgaaccgcgc atagcatccc tggcattcga
      781 attgctcgcc aatgttcccc agcggaatga cgggaatgaa gtagagcgta aaccagcggt
      841 tcatcaccac gtgccggcag gcctgctgcg tgcggcagtt cggacaggcg aacaggccct
      901 gccccagggt cgattccgtc gtccgcatgc cgtagatgat cacgctgcac gtccccccga
      961 tcgaaaactg cacctggcga tctgctccag gataagaaac acgcgagcca ggtgcgagcg
     1021 ttcgatcgac cggtttccag gttatggccg gcggatgccg tcgagcaccg ccgccgtcgc
     1081 gggggatttg ttgaggacgt agaagtggat gccggggatg cctgcgtcga tcagctcctg
     1141 gacctggcgg gtggcctggc aaacgccggc ttggaactgc cagtcgggat cgtccttctg
     1201 cgagagctcg gcgacgaagt cggctgggag gcaggctttg cagagggagg tgatgcgctg
     1261 gatctgggcg aggttcgtca cgggcaggat gccggggacg atcggggccg tgatgcccag
     1321 ctcctggcag cggtcgcgaa agcggaagaa gtcggcgttg ctatagaaga gctgcgtgat
     1381 gaccacctcg ccgcccgctt tcactttgcg ggcgaggttt tcgaggtccg cttgcgggct
     1441 cgtcgcttcc tggtgcgtct ccggatatcc ggcgaccgcg atgccgaacg aggggaattc
     1501 gcggcgaatc agctcgacca gttcgctggc gtagcgcaag ccgccttcga ccggcttgaa
     1561 agccgtttcg cctttgggcg gatcgccgcg gagggcgacg atgttctcga tccccgcgcc
     1621 gcgggcctcg cgcaaatagc cgcgaagctg atcggtcgtc gagccgacgc acgtgaggtg
     1681 cgaggcgacg cggacgccga accgccgacg gacctgcgtc gtgatttcca gcgtcttatc
     1741 gcgcgtcgag ccccccgcgc cgaacgtgca ggtgatcaca tccggctgaa aggccatcag
     1801 gtggccgacg ttgtcgtaca gcgacgcctc gcccgccgcg gttttgggcg ggaagagctc
     1861 gaaggagaga gtgaacgggc gagcggggta aaagggcatt gtggaatgcg gaattcggaa
     1921 tgcggaatgc ggagtggttg cgattcatat tagaggttct aacacaacct ggaattgaca
     1981 ttattcgcgt ggtcagattt tggtattctt cgcggtggga ttagcttcgt ggtctctgtc
     2041 atggaggaca ctgcga
//
LOCUS       DASUGB010000895         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1211404, whole genome shotgun sequence.
ACCESSION   DASUGB010000895 DASUGB010000000
VERSION     DASUGB010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211404"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..>2368)
                     /locus_tag="VFV87_17040"
     CDS             complement(163..>2368)
                     /locus_tag="VFV87_17040"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105529.1"
                     /translation="LLTGRLQATYFNATVMPIAYDFERNTQGQVYTIRKELKRIDPGE
                     RIIVEVVDYDADQSADRDTIQIEVAVNDGQPVVLEATETEAYSGIFTKEVDTSAKSEK
                     DKLQVKAGDRVAIRYFDEHNTFPGHSVPREQVVYVAQSTPALVRVLETRVIPPPKDSK
                     APPQITVLPPKKEQETSGVAFEAPLTVEVIDPDAAKDSRSSAVVKLQTTDGAIVEVEC
                     RVSPMFSDIVGDNPERQALEEGRFVGQVILQLGGKASPAVVPITSEMPRNLIGKVRLG
                     EEAGEDTLGANLVTRVLSVSGKDIVTATYNDARRPEGKPADIAAKGRLISNGVLAVTD
                     RDYENEMTQLHVGERLYLQVTDPDLDASDARDTAKIEITTELGDKETVQLEETLSHSG
                     AFTGSLQLKAADTPTPGNFDPANPAVESYFGDNVTVRYVDPAASTETGTLELTVTLPV
                     VVGTDGLVAAFSKTFNDENLAVETKFRIAESYFELFKSHKELQRDEEKKIDLEAGRRV
                     LREVMEDYPDPKYAPRISYLLGQFAQELEQWNEAIRSYDLILRQYPDHTLAPDAQYKL
                     AQCYEQAGDFDQALEAYVTLAATHPKSPLIPNVMIRISDYFYKNEQFEVAAQVGEKFL
                     ERFQEHQHAPRMAFRVGQCYFKSKKYTIAGKAFDNFAEIFPKDALGADAFFWAGESYR
                     MANNNRQAFISYNNCRWKHPDSEAAKYARGRLALPEMLQQFEAEANSVDNDN"
BASE COUNT          381 a          736 c          733 g          518 t
ORIGIN      
        1 gccaggatgt catcggcctg accggcgatc agcagaggac tcaggatcca catggcgcaa
       61 gttccgatct atcgagtttc actcaccact cacctactca cctgggaacc gcgactctgg
      121 tcgcggaatt gtgcctgacc gcgaccactg gtcgcggttt tgtcaattgt cgttatccac
      181 gctgttcgcc tcggcctcga actgttgcaa catttcgggc agggcaagcc ggccgcgggc
      241 atatttggcc gcttcgctgt cggggtgctt ccagcggcag ttgttgtagc tgatgaacgc
      301 ctggcggttg ttgttcgcca tgcggtagct ttcgccggcc cagaagaacg catccgcgcc
      361 cagggcgtcc tttgggaaaa tctcggcgaa gttgtcgaac gccttgccgg cgatcgtgta
      421 cttcttgctc ttgaagtagc actggccgac gcgaaaagcc atccgcggcg cgtgttgatg
      481 ctcctggaac cgctccagga acttctcgcc gacctgggcc gccacctcga actgctcgtt
      541 cttatagaag tagtccgaaa tgcggatcat cacattgggg atcagcgggc tcttgggatg
      601 cgtagcggcc agcgtcacgt acgcctccag cgcctggtca aaatccccgg cctgctcgta
      661 gcactgggcc agcttgtact gggcgtcggg cgcgagcgtg tggtcgggat actgccgcag
      721 aatcaggtcg tacgagcgga tcgcctcgtt ccactgctcc aattcctggg cgaactggcc
      781 gagcaggtac gaaatccgcg gggcgtactt cggatcggga taatcctcca tcacctcccg
      841 cagcacgcgg cgcccggctt cgaggtcgat cttcttttcc tcgtcgcgct gcaactcctt
      901 gtggctcttg aacagctcga agtagctctc ggcgatgcgg aatttcgtct cgaccgccag
      961 gttctcgtcg ttgaaggttt tgctgaacgc cgcgaccagg ccatcggtgc cgacaaccac
     1021 cggcagcgtc acggtcagtt ccagcgtccc cgtttccgtg ctggcagccg gatcgacgta
     1081 acgcacggtc acgttgtcgc cgaaatagct ctcgactgcc ggattggccg ggtcgaaatt
     1141 gcccggcgtc ggcgtatcgg ctgcttttag ttgcaaggag ccggtgaaag cgccgctgtg
     1201 ggagagcgtt tcttcaagct ggactgtttc cttgtcgccc agctcggttg tgatctcgat
     1261 tttcgccgtg tcgcgggcgt ccgacgcatc cagatcggga tcggtcactt gcagatagag
     1321 ccgctcgccg acgtggagct gcgtcatctc gttctcataa tcccgatcgg tcaccgccag
     1381 cacgccgttg ctgatcaagc ggcccttcgc ggcaatatcc gccggcttgc cttcgggacg
     1441 gcgggcgtcg ttatacgttg ccgtgacgat gtccttgccc gacacgctca gcacgcgcgt
     1501 cacgagattc gcgccgagcg tgtcttcgcc cgcttcctcg ccgaggcgga ccttgccgat
     1561 cagattgcgg ggcatctcgg acgtaatcgg cacgactgcg gggctggcct tgccgccgag
     1621 ttgcaggatc acctgaccga cgaagcggcc ctcttccagc gcctgccgct cggggttgtc
     1681 gccgacgata tcggaaaaca tgggcgaaac gcggcattca acctcgacaa tcgcgccgtc
     1741 ggtcgtttgc agcttgacca cggccgagct gcggctgtcc ttggcggcat cggggtcgat
     1801 cacttcgacg gtaagcggcg cctcaaaggc gacgccgctc gtttcctgct ctttcttggg
     1861 cggcaggacg gtgatctgcg gcggggcctt ggagtccttg ggtggcggga tgacgcgcgt
     1921 ttccaggacg cggaccaagg ccggcgtcga ttgggcgaca taaacgacct gctcgcgcgg
     1981 cacactgtgg ccggggaacg tgttgtgctc gtcgaaatag cgaatggcga cgcggtcgcc
     2041 cgctttcacc tgcagcttgt ccttctccga cttggccgaa gtatccactt ccttggtgaa
     2101 gatgccagaa taggcttcgg tttccgtcgc ttcgagcacg accggctggc cgtcgttgac
     2161 cgcgacctcg atctgaatcg tgtcgcgatc ggccgactgg tcggcgtcgt aatcgaccac
     2221 ttcgacgatg attcgctcgc ccggatcgat ccgcttcagc tctttgcgga tcgtatagac
     2281 ctggccttgg gtgttccgct cgaagtcgta ggcgattggc atgaccgtcg cgttgaaata
     2341 ggtcgcctgc agccgtcccg tgagcagg
//
LOCUS       DASUGB010000896         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_934792, whole genome shotgun sequence.
ACCESSION   DASUGB010000896 DASUGB010000000
VERSION     DASUGB010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934792"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="VFV87_17045"
     CDS             <1..1370
                     /locus_tag="VFV87_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105530.1"
                     /translation="NPLVGWSVVVMMFLKAYSLGAGTYTGIEAVSNSMAVMREPRVAT
                     AQRTMFYMAVSLAVMAGGLMLAYMFFELELRQRATDLLTLKHRTGRTMNDLVMEWFTS
                     RAFPAPIARLFRWIAVLSEATLLLVAAQAGFIGGPKCLATMAHDSWVPHWFGSLSERL
                     ASHNGIILIGLSALAALWANDGNVSSLLIMYSINVFLTFSLSMIGMTLHWYHHRGKHP
                     RWRQRMALFIFGAVLCVSILIVTVAFKFAEGAWMTVLATGLITLLALLVNQYYTSVRQ
                     RLKGLDESLGTIEIKGEPNLAPPDPEQHTAVILVGGYSGLGVHTLLNSVRFVPNHFKN
                     MIFISVGVVDSGNFKGAEEVEHLRSYTESALERYVELARRLGFPSRAYMAIGTDVVDE
                     LEQLCRTVHRDFPKAVVFAGQLVFQRETWYGALLHNQTAYSLQRRLQWDGVPMVILPT
                     RVRER"
     gene            complement(1426..>2145)
                     /locus_tag="VFV87_17050"
     CDS             complement(1426..>2145)
                     /locus_tag="VFV87_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5105531.1"
                     /translation="EDGDSLDATLVGDDPATDLALVRLAARDLPVAELGDSNALQVGQ
                     LVIAMGHPLGFRSTVSTGVVSALGRAMRSEQGQPIENVIQHTAPLNPGNSGGPLVDSH
                     GRVVGINTAIIAMAQGLGFAVPATTARWVADELLTHGRVRRASLGIVAAVVDLPRQLV
                     RDLDLLADQAVAVAEVIAGGAAAGAGLAEGDLIVAAAGRLIVGTDDLHRVLAALPAGQ
                     HVVLEVVRHARLLQIDVQPQM"
BASE COUNT          346 a          746 c          662 g          391 t
ORIGIN      
        1 acaacccgct cgtcggctgg agcgtcgtgg tcatgatgtt cctcaaggcc tactcgctgg
       61 gcgcgggcac gtacacgggc atcgaggccg tgtccaacag catggccgta atgcgcgagc
      121 cgcgggtcgc cacggcacag cggacgatgt tctacatggc ggtctctttg gcggtcatgg
      181 ccggcggatt gatgctggcc tatatgtttt tcgagctgga actgcggcag cgggccaccg
      241 acttgttgac gctcaagcac cgcacgggcc ggacgatgaa cgacctcgtc atggagtggt
      301 tcaccagccg cgcgtttccc gcgcccattg cccgcctgtt ccgctggatc gcggtgctgt
      361 cggaggcgac gttgctgctc gtggccgccc aagccggttt tatcggcggc cccaaatgcc
      421 tggccaccat ggcccacgat tcctgggtcc cgcactggtt cggcagcctc tcggagcggc
      481 tcgcgtcgca caacggaatc attctcatcg gcctctccgc tctggctgcc ttgtgggcaa
      541 acgacggcaa cgtctcctcg ctcttgatca tgtactcgat caacgttttc ctgacgttct
      601 cgctgtcgat gatcggcatg acgctgcact ggtatcacca ccgcggcaaa catccccgct
      661 ggcggcagcg gatggcgctg ttcatctttg gggccgtgct gtgcgtttcg attctcatcg
      721 tcaccgtcgc gttcaagttc gccgaagggg cctggatgac cgtgctggcc accggcctga
      781 tcacgctcct ggccctgctc gtgaaccagt actacacgag cgtccgccag cggctcaaag
      841 ggctcgacga atcgctcggc accatcgaaa tcaagggcga gccgaatctc gccccccccg
      901 accccgaaca gcacaccgcg gtcatcctgg tcggcggcta cagcggcctg ggcgtccaca
      961 cgttgctcaa ctcggtccgt tttgtgccca atcatttcaa gaacatgatc ttcatctcgg
     1021 tcggcgtcgt cgattcgggc aatttcaagg gggccgagga ggtcgagcac ctgcggagct
     1081 acaccgaaag cgccctggag cgctatgtcg agctggctcg ccgcctgggt tttccctcgc
     1141 gggcctacat ggcaatcggc accgacgtgg tcgatgagct cgagcagctg tgccgaacgg
     1201 tccaccgcga ttttcccaag gcggtcgtct tcgccggcca gctcgtcttt cagcgcgaga
     1261 cgtggtacgg cgcgctattg cacaaccaga cggcctactc gctccagcgc cgcctccagt
     1321 gggacggcgt gccgatggtc atcttgccga cgcgcgtccg agagcggtaa gtgcagcctc
     1381 tgactgcttc ggcccaagcg gcctttcggg caagatcgcc tctggctaca tctgcggctg
     1441 cacgtcgatc tgcaacagcc gcgcgtggcg gacgacttcc agcaccacgt gctgaccagc
     1501 gggaagcgcc gcgagcaccc ggtgcaaatc gtcggttcca acgatcaggc ggcctgccgc
     1561 cgctacgatc aagtcccctt cggccaggcc ggcgccggcg gcggctccgc cggcgatcac
     1621 ctcggcgacc gcgaccgcct ggtccgcaag caggtccagg tcgcgcacca attgccgggg
     1681 caaatcgacc actgcggcca caatgcccaa cgatgcccgc cgcacgcggc catgggtcag
     1741 caactcgtcc gcgacccagc gggccgtcgt tgccggcacg gcaaagccaa gcccctgagc
     1801 catcgcaatg atcgccgtat tgatgccgac cacccgtccg tggctatcga ccagcggccc
     1861 gccggaattg ccgggattca gcggcgcggt gtgctggatt acgttctcga tcggctgtcc
     1921 ttgctcgctg cgcatcgccc gccccagcgc cgagacgacg ccggtcgaaa ccgtcgagcg
     1981 aaatcccagc gggtgcccca tcgcgatgac aagctggccg acctgcaggg cattgctgtc
     2041 gcccagctcg gcgaccggca gatctcgggc ggccaggcgg acaagcgcca agtcggtcgc
     2101 cggatcgtcg cccacgagcg ttgcgtccag gctgtcgccg tcctc
//
LOCUS       DASUGB010000897         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_934861, whole genome shotgun sequence.
ACCESSION   DASUGB010000897 DASUGB010000000
VERSION     DASUGB010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934861"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1113
                     /locus_tag="VFV87_17055"
     CDS             58..1113
                     /locus_tag="VFV87_17055"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5105532.1"
                     /translation="MAEHVVIIGSGPAGWAAAIYASRANLQPLVFEGAETEENRISGT
                     LPLGQLNLTTEVENYPGFPAGLLHHFLRSALPEDRLQTFLEPLHEHKHAVTGPELMHL
                     MRQQAVNFGTRIVTDDVLEVDLSRQPYKLKTLGGETHQSHTLIIATGARANYLGLPSE
                     ERYKNRGVSACAVCDGALPRFRNKPLVVVGGGDSAVEEATYLTKYASTVHMLMRRDVF
                     RASKIMQDRALSNPKINIQYFREVDEVLGDDKQGVTGIRMKDNQTGGREDIPASGLFL
                     AIGHTPNTTFLKGQLELNDKGYIKYTTLFRTYTSKEGVFAAGDVADDYYRQAVTSAGT
                     GCMAALDAERYLAAKGL"
     gene            complement(1182..1853)
                     /locus_tag="VFV87_17060"
     CDS             complement(1182..1853)
                     /locus_tag="VFV87_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5105533.1"
                     /translation="MTELIDFVLHFDDKLAMIIAEYGAWTYAILFAIIFCETGLVVTP
                     FLPGDSLLFAVGVFAGKPGQPLDIWTATILLTIAAILGDTVNYHVGKYLGSRILTGKL
                     SRWLNPKHLERTHWFFERYGGKTIVLARFVPIVRTFAPFVAGVGSMSYTKFLTYNVLG
                     GIAWVVICTQAGWWLAGNAFVEEHFEIVVLIVVAISLLPALVEILIHRYSRKPSVVVS
                     ESTVP"
     gene            complement(1924..>3482)
                     /locus_tag="VFV87_17065"
     CDS             complement(1924..>3482)
                     /locus_tag="VFV87_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105534.1"
                     /translation="AALLGWMFDGAEMGLFSMVGRPAIKDLLQTTDEAVVGLWFSVIT
                     ASFLVGAATGGVLFGWLGDRIGRVRAMALSVLTYALFTGLCGIAGTPIQVGVLRFIAS
                     LGMGGEWSLGVALVMEVWPNRSRAFMAGLIGAAANVGYLLVGLVGFVLLDVIGALQDG
                     MLAIGLRESWVEMLVRNNGWRLMMIMGTLPAILTFFIRMFVPESAKWQKEHAAGSTGH
                     WATQDLIAVVIGLVGPAMIVTIWAWPVKSPSLTWTLIQAAVTLVGLAIAVVGYTYPVV
                     RYFQRLHLAGSGEDWQPTLRRMLLGACLSGVALLGTWGSTQWAPSWADSLTEKKHQAQ
                     QKALIDDGKEEEAQALVRPRAKEFTQISLAIGAIVGTILAAMMGDWIGRRAAYCLLCA
                     LSLGSVYALFLGNSEYGPMMLFWTFIAGTLTASFYGWLPLYLPELFRTKVRATGQGFG
                     FNFGRILAAIGVLQTGNLMGLFKDGVTIGGIDIPGGYPLACSTMSLVYLIGIGIIWLA
                     PETRGQPLPD"
BASE COUNT          728 a         1126 c         1086 g          542 t
ORIGIN      
        1 cgctgctaga attcgggcga ctttggacct tggacttcgg actgaaagga ctgacggatg
       61 gccgagcatg tggtgatcat cggcagcggg ccggcgggat gggcggcagc gatttacgcg
      121 tcgcgggcca acctgcagcc gctggtgttt gaaggggcgg agaccgagga gaaccggatt
      181 tccggcacgc tgccgctggg gcagctcaac ctgacaaccg aggtcgagaa ttatcccggc
      241 ttcccggctg gcttgctgca ccattttctt cgcagcgctc tgccggagga ccggctgcag
      301 acgttcctgg agccgctgca cgagcacaag cacgccgtca cgggccccga gctgatgcac
      361 ctgatgcggc agcaggcggt gaacttcggc acgcggattg tcaccgacga cgtgctggag
      421 gtcgatctca gccgccagcc gtacaagctg aagacgctgg ggggcgagac gcaccaatcg
      481 cacacgctga tcatcgccac cggcgcgcgg gcgaactacc tcggcctgcc gtcggaagag
      541 agatacaaga accgtggcgt cagcgcctgc gccgtctgcg acggggccct gccgcggttc
      601 cgcaacaagc cgctggtcgt ggtcggcggc ggcgattccg cggtggagga ggcgacgtac
      661 ctgaccaagt atgcttcgac cgtgcatatg ctgatgcgcc gcgacgtgtt ccgggccagc
      721 aagatcatgc aggaccgggc gctatccaac cccaagatca acattcagta tttccgcgag
      781 gtcgatgagg tgctgggcga cgacaagcaa ggggtcaccg gcatccgcat gaaagacaat
      841 cagaccggcg gacgcgagga tattcccgcc agcggcctgt tcctggcgat cggccacacg
      901 cccaacacga cgttcctcaa gggacagctt gaattgaacg acaagggcta catcaagtac
      961 acgaccctct tccgcaccta caccagcaaa gagggcgtct tcgccgccgg cgacgtggcc
     1021 gacgactact accgccaggc cgtcacctcc gccggcaccg gctgcatggc ggcgctggat
     1081 gcggagcggt atttggccgc taaaggcctg tagggaacgg cctctgtgcc gttccggagc
     1141 gcggcgggca cggaacgcca cggagggcgt tccctacagc gctatggaac cgtcgattcc
     1201 gagacaacca cgctcggctt gcggctgtag cggtggatca ggatttcaac gagcgcgggc
     1261 aagagcgaga tcgcgacaac gatcagcacg acgatctcga agtgctcctc gacgaaggcg
     1321 ttgccggcca gccaccagcc ggcttgcgtg cagatgacaa cccaggcgat gccgccgagg
     1381 acgttgtacg tcaggaactt cgtgtaggac atgctgccga cgccggcgac gaacggggcg
     1441 aatgtccgga cgatcggcac gaagcgtgcc aggacaatcg tcttgccgcc gtagcgctcg
     1501 aaaaaccaat gcgtccgctc caggtgcttg ggattcagcc agcgcgagag cttgccggtc
     1561 agaattcgcg aaccaagata cttgccgacg tgatagttga ccgtgtcgcc caggatcgcg
     1621 gcaatcgtga gcagaatcgt cgccgtccag atatcgagcg gctggccggg cttgccggca
     1681 aagacgccga cggcgaacag cagcgaatcg cccggcagaa agggggtcac caccagcccc
     1741 gtttcgcaaa agataattgc gaacaggatc gcgtacgtcc aagcgccata ctcggcgatg
     1801 atcatcgcca gcttgtcgtc gaagtgcagg acgaaatcga tcagctcggt catgacggct
     1861 atcgcggcgc agggtcgttc tcccgccaac tatagctcat gtcgcgaaat tgccccaggg
     1921 cgattaatcc ggcagcggct ggccgcgggt ctccggggcg agccaaataa tgccgatgcc
     1981 aatcagatag accaggctca tcgtggagca ggccagcgga tagccgccgg gaatatcgat
     2041 tccgccgatg gtcacgccgt ccttgaacag gcccatcagg ttgccggttt gcaacacgcc
     2101 gatggccgcc agaatccggc cgaagttgaa accgaagccc tgaccggtcg cgcgtacctt
     2161 ggtgcggaaa agctccggca gatagagggg cagccagccg taaaacgagg cggtcagcgt
     2221 gccggcaata aaggtccaga agagcatcat cggcccgtat tcggaattgc caagaaacag
     2281 ggcgtaaacc gatccgagcg acagtgcgca caacaagcaa tatgcggctc gacgtccgat
     2341 ccaatcgccc atcatggcgg ccaggattgt tcccacgatg gccccgatcg ccagcgagat
     2401 ttgcgtgaac tctttggccc ggggacgtac cagcgcctgg gcttcctcct ccttgccatc
     2461 gtcgatcagg gccttttgct gagcctgatg cttcttttca gtgaggctgt cggcccagga
     2521 gggcgcccac tgcgtggagc cccacgtgcc gagcagcgcc acgccgctca ggcaggcgcc
     2581 cagaagcatc cggcggagcg tcggctgcca gtcctctccg ctgccggcca ggtgcagccg
     2641 ctgaaagtag cggacgacgg gatacgtgta gcccacgacg gcaatcgcca aaccaacgag
     2701 cgtcacggca gcttgaatca gcgtccaagt gagcgaaggc gacttcaccg gccaggccca
     2761 gatcgtgacg atcatcgccg ggccgacgag cccaatgact acggcaatca ggtcttgggt
     2821 cgcccagtgg ccggtgctgc cggcagcatg ttccttctgc cactttgccg attcggggac
     2881 gaacatgcgg atgaagaaag tgaggatcgc cggcagcgtg cccatgatca tcatcagccg
     2941 ccagccgttg ttgcggacga gcatttcgac ccaggactcc cgcaggccga tcgccagcat
     3001 tccatcctgc aaagccccaa tcacatcgag cagcacaaaa ccgacgaggc ccacgagcag
     3061 gtagcctacg ttggcggctg ctccgatcag gccggccatg aacgcccgcg agcggttcgg
     3121 ccagacttcc atcaccagcg ccacgccaag cgaccattcc ccgcccattc ccagcgaggc
     3181 gatgaatcgc aacacgccca cttgaatcgg ggtgccggcg atcccacaca gcccggtgaa
     3241 caaggcatac gtcagcacgc tgagggccat tgcccgcacg cggccgatcc ggtcgcccag
     3301 ccagccgaac agcacccccc ccgtggccgc ccccaccaga aaactggcgg tgatgaccga
     3361 gaaccacagt ccgacgaccg cttcatccgt tgtttgcagc aagtccttga tcgccgggcg
     3421 accgaccatc gagaacaggc ccatctcggc tccgtcgaac atccacccca gcagcgcggc
     3481 cg
//
LOCUS       DASUGB010000898         6766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1906234, whole genome shotgun sequence.
ACCESSION   DASUGB010000898 DASUGB010000000
VERSION     DASUGB010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906234"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /locus_tag="VFV87_17070"
     CDS             <1..2016
                     /locus_tag="VFV87_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX5105535.1"
                     /translation="FDLAELESQAATLVWNSGQERFAWKHDDLAGLPLVVESPPDDDR
                     FTQIVRSVGRLAKDTSRVEVPFETVLPSAGQWWTADSRAEIEVPLGRAGAMKLQHLRL
                     GKGTSQHVLIAGKTGSGKSTLLNAMITNLAAHYSPDELEFYLIDFKKGVEFKAYAANR
                     LPHARVIAIESEREFGLSVLERLDLELKRRGDLFRERGVQDLKGYRTANPAARMPRIL
                     LIIDEFQEFFTSDDKISHDAALLLDRLVRQGRAFGIHVLLGSQTLAGAYSLARSTIGQ
                     MAVRIALQCSESDAHLILSEDNTAARLLGRPGEAIYNDANGLFEGNHPFQVVWLSDHE
                     REAHLQRVAELAAERTVNVPAPVVFEGNVPASIAENTALCELLQSPPPNSQPLAPRAW
                     LGAAVAIKEPTEVAFRRQGGSNLLIVGQHEELALGVLASCLVSLGAQAPRVGQVSNLS
                     EDKLETHPTTVDGLETRPTNRTRFWILDGTRPESPEAGFWRRVTGELPLDAEVSLPRD
                     AASVVTQLAEEVDRRLANSDLALAPIFLLVSNLSRFRDLKKPDDFASFDDDGSTVAGK
                     HLTTILREGPGVGVHAVVWCDSYNNLNRWLDRQSLRDLELRVLFQMSPTDSSNLMDSP
                     AASRLGVHRAIFYSEEQGQAEKFRPYGLPSAEWLDWAGQQLGRSQES"
     gene            2406..4241
                     /locus_tag="VFV87_17075"
     CDS             2406..4241
                     /locus_tag="VFV87_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5105536.1"
                     /translation="MSLLRFATLFLLVILSCAPSRAASPLSELEPAQRPRLKSVYSTI
                     HDLTEFTKGDTRAVVVVFLGTECPVSLQYVPRLNELSEEFRAQNIKFLGVYPDVGVNL
                     HEMATHSHDADIVFPVFKDVDLHLADWLGAQVVPEVVVLDKDLKKVYQGAIDDQYKRG
                     GRRAEANDEYLHDALAALLGGKEVETEYIPASGCPIERTVPARAPRDLSFHKDVEPIF
                     QRHCQECHRDGGPGPFELLTFDDIAYNTEKIREVVIDRRMPPWHGFLNPKYGKIANDQ
                     SLTPEELETIVAWIDGGAKQGNAADAPPPVVWPAPEAWGIGQPDFVFKMNEPFRVPKT
                     GIIEYQFFRVKLDYDDDRWFRGIEIKPGNREVVHHVTLHLAPSLRDDKFKGFASMAQL
                     YGLSGENAHLINDYVPGDSYNAKVYPPHQAVKIPKHSDLIFEVHYTPNNREAVSDQSM
                     VAFQWGAGTPAEEVLTRVFRKPTGRFKVPPYAGHYVVEDTYYFENDVVIEAIRPHMHL
                     RGKKWRLERIERNPETDEIESRETILSVPIFDPGWQRTYELALPFVLPAGTELVATAV
                     FDNTRLNPDNPDPSAEVVWGQQTTDEMFSTRFKYSLAKKPAGQEH"
     gene            4244..6103
                     /locus_tag="VFV87_17080"
     CDS             4244..6103
                     /locus_tag="VFV87_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105537.1"
                     /translation="MRWLANLGLALLLASIVPLAADGRSPQPAAVVQALTQAAQNAPQ
                     PQESVGEALGRLKNSLQELDAVLLRNDEAIRRGWSEYLDLPAIQAELTRPQPDTALLE
                     ARSKRFYQNHVGLERSQFIDVRSELRGYLIAREYAVMDQPLEEYRQRVTELSDCLARI
                     DASFDEADAHRAGVLANWLAPLSDEGANLADAVRENYCRTNGVGHVSSRAINLLMERS
                     VKEQNYIQETMMGALTRGTAYMNGQVTMAVVPNSTQASLEIRLAGQVSVPSTVSQRGQ
                     ITAYSSSNSGLYATKQVNIDRNGLSLRPAATASNTNMQIQDVVAPSRIIERLAWRRAN
                     RMLPEAQTAANRRTQTEINTNLDEQGAAMLSGVNDTFLNKVCWPMLRANTLPPEMRFW
                     TDSTHLRLAFSQWNDRQLAPASPVPQLLDEYDMACGAHESMINNFTEVLLGGLTSKDE
                     GWAHVMEIMTGSVPRPLWVHDRAPRWTATFEKERPFIAHFDGGRCSFTLRLTKVTQGD
                     KVYEHPVEVEAHMIPRITKDGPALFRDGDLDIRVAPGMQSQAEAELRKLLARKVGAVF
                     PPELYFDGLAPPAGGTIGRLRQVQISEMRCEDGWMTLGYKLQTAPVEVAGGGS"
     gene            complement(6134..>6766)
                     /locus_tag="VFV87_17085"
     CDS             complement(6134..>6766)
                     /locus_tag="VFV87_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5105538.1"
                     /translation="LVLQEEMRERAAKLWDCKPDEVEVVGGTYSNNGNRLAFKELAAK
                     ISQKNLPPVVGQASVAPEGSTNGFGTHICDVEVDPETGKVTILRYTAIQDAGKAVHPS
                     YVEGQMQGGAAQGIGWALNEEYWLDKEDRMRNASFLDYRMPTCLDLPMIETIIVEVPN
                     PSHPFGVRGVGETPIVPPPAAVANAIHAATGKRLTELPMSPPKVWKALSS"
BASE COUNT         1263 a         2179 c         2092 g         1232 t
ORIGIN      
        1 ttcgatctgg ccgaactcga atcgcaagcc gcgacgctcg tctggaactc gggccaagag
       61 cggttcgcct ggaagcacga cgatctggcc ggcttgccgc tcgtggtcga atcgccgccc
      121 gatgacgacc gctttaccca gatcgtgcgc tcggtcggcc gcctggcgaa ggacacttcc
      181 cgtgtcgaag tgccgttcga gaccgtcctc ccctccgccg gccagtggtg gaccgccgat
      241 agccgggccg agatcgaagt gccgctgggc cgcgccgggg cgatgaagct gcagcacctg
      301 cggctgggga aggggacatc gcagcacgtg ttgatcgcgg gcaagaccgg ttcaggcaaa
      361 tctacgctgc tcaacgcgat gatcaccaat ctcgcggccc actattcgcc ggacgagttg
      421 gagttctacc tgatcgactt caagaagggg gtcgaattca aggcctatgc tgccaatcgc
      481 ctgccacacg cccgcgtgat cgcgatcgag agcgagcggg aattcgggct ctcggtgctg
      541 gaacgactcg acctggagct gaagcgccgc ggcgatctgt tccgcgaacg tggcgtgcag
      601 gacctgaagg gctatcgcac ggcaaatccc gctgctcgaa tgccgcggat tctgctgatc
      661 atcgacgagt ttcaggagtt cttcaccagc gacgacaaga tctcgcacga tgcggctttg
      721 cttctcgatc ggctggtgcg gcaggggcgg gcgtttggca ttcatgtgct gctcggctcg
      781 caaacgctgg ccggggccta ttctttggcc cgcagcacga tcggccagat ggcggtccgc
      841 attgccctgc agtgcagcga atccgatgct cacttgatcc tcagcgaaga caacacggca
      901 gcccgcctgc tcggccggcc gggcgaagcg atttacaacg acgccaacgg cctgtttgaa
      961 gggaatcacc cgtttcaggt cgtctggctg tcggaccacg agcgcgaagc gcatctgcag
     1021 cgggttgcgg agttggccgc cgagcgaacg gtgaatgttc ccgcaccggt tgtcttcgaa
     1081 ggcaatgtgc cggccagcat cgcggagaac acggccttgt gtgagctgct ccaaagtccg
     1141 ccacccaatt cgcagccact cgccccgcgg gcatggctcg gcgccgcggt cgccatcaag
     1201 gagccgaccg aagtcgcctt ccgccggcag gggggcagca atctgctgat cgtcggtcag
     1261 cacgaggaac tggcgctggg cgtgctggcg agctgcctgg tttcgcttgg cgcccaggcg
     1321 ccgcgcgtag gacaggtttc caacctgtcc gaagacaagc tagaaaccca tcctacgaca
     1381 gtggacgggt tggaaacccg tcctacgaac cgcacgcgct tctggatcct cgacggcacg
     1441 cggcccgaat cgcccgaggc gggtttttgg cgacgagtaa cgggcgaatt gccgctggat
     1501 gcggaagtct cgctgccacg cgatgcggca agcgtcgtaa cgcagctcgc cgaggaagtc
     1561 gatcgccgat tggccaatag cgatctggcc ttagcaccaa tcttcctcct ggtcagcaat
     1621 ctctcccgtt tccgcgatct caagaaacct gacgactttg caagcttcga cgatgacggt
     1681 tcgaccgttg caggaaagca tctgacgacg atccttcgtg aaggtcctgg tgtcggcgtt
     1741 cacgcagtcg tctggtgcga ttcgtacaac aacctgaacc gctggctcga ccggcagtcg
     1801 ctccgcgatc tcgaattgcg cgttctcttt cagatgagcc caaccgattc gagcaacctc
     1861 atggacagcc ctgctgccag ccgtttgggc gtccaccgcg ccatctttta cagcgaagag
     1921 cagggccagg ccgagaaatt ccggccctac ggcctgccct ctgcggagtg gctggattgg
     1981 gccggccagc agcttggccg cagccaggaa tcgtagcggc gaactctccg gaagcaggga
     2041 aagtcttgcc ggtcaggcaa tcctgccacg acgcgcgccg gaaattgacg ccggtaaatc
     2101 cggtgaggac ggacctgccg tagaagttgc acttgccagc aggacgtggg gcgatactca
     2161 aggaccgccg tcagccgata tttcaaatgc ttcttcccct tcgccgcagc gcgccggctg
     2221 aggggcacgc gggttgcgaa atgccacttt tcaggcgggg caaaaccact ctccccttgc
     2281 acacccgccg tcgatagtac gtaattatca tggcttttat ggttgttttt gaccgtgtga
     2341 ctttatgcgg accccgaccg gccggatcca tcggcttggc tctgggactc gttgggaggg
     2401 ccggcatgtc tctgcttcgt ttcgcaacat tgtttttgct ggtgatcctc tcatgtgcgc
     2461 ccagccgtgc tgcctcgccc ctgtccgaac tggaacccgc ccagcgcccg cggctgaaga
     2521 gcgtctattc cacaatccac gacctgaccg agttcacgaa gggcgacacg cgggcagtcg
     2581 ttgtcgtgtt cctggggacg gaatgtccgg tctcgctgca gtacgttccg cgactgaatg
     2641 agctctccga ggagtttcgc gcgcagaaca tcaagttcct gggtgtttat ccggacgtcg
     2701 gcgtgaacct gcacgagatg gccacgcact cccacgacgc cgacatcgtc ttcccggtct
     2761 tcaaggacgt cgatctgcac ctggctgatt ggctcggcgc gcaggtcgtg cccgaggtcg
     2821 tcgtgctcga taaggacctg aaaaaggtct atcaaggcgc gatcgacgat caatacaagc
     2881 gcgggggccg ccgcgccgag gccaacgatg agtacctcca cgacgcgctc gccgccctcc
     2941 tcggcggcaa ggaagtggaa accgagtaca tccccgcctc cggctgcccg atcgagcgca
     3001 ccgtgccggc acgcgcaccg cgcgatctga gcttccacaa ggatgtcgag ccgatcttcc
     3061 agcgccactg tcaggaatgc catcgcgacg gcggccccgg cccgttcgag ctgctgacct
     3121 tcgacgacat cgcctacaac accgagaaaa tccgcgaagt cgtgatcgac cgccgcatgc
     3181 cgccgtggca cggcttcctg aatcccaagt acggcaaaat cgccaacgac cagagcctga
     3241 ctcccgaaga gctggagacg atcgtggcct ggatcgacgg cggcgccaaa caggggaatg
     3301 cggccgacgc cccgccgccc gtcgtgtggc ctgcgcccga ggcctggggc atcggccagc
     3361 cggactttgt cttcaagatg aatgagccgt tccgcgtgcc gaagacgggc atcatcgagt
     3421 accaattctt ccgcgtgaag ctcgactatg acgacgaccg gtggtttcgc ggcattgaga
     3481 tcaagccggg caaccgggaa gtcgtacacc acgtcacgct gcacctggcg ccgtcgctcc
     3541 gggacgacaa attcaagggc ttcgcttcga tggcccagct ttacgggcta agcggtgaaa
     3601 acgcccatct gatcaacgac tatgtgcccg gcgattccta caacgccaag gtctatcctc
     3661 cgcatcaggc ggtgaagatt cccaagcact ccgacctgat ctttgaggtg cactacacgc
     3721 cgaataaccg cgaggcggtg agtgaccagt cgatggtggc cttccagtgg ggcgcaggca
     3781 ctcccgccga agaggtgctg acccgcgtct ttcgcaagcc gactggccgc ttcaaagtgc
     3841 ctccctacgc cggccactac gtcgtcgaag acacctatta cttcgagaac gacgtggtga
     3901 tcgaggcgat tcgcccgcac atgcacctcc gcggaaagaa gtggcgcctg gagcggatcg
     3961 agcggaatcc cgaaaccgac gagattgaaa gccgcgagac gatcttatcc gtgccgattt
     4021 tcgatcccgg ctggcagcgg acctatgagc tcgctttacc ctttgtgctt cccgcgggga
     4081 ccgagctggt cgcgaccgcc gttttcgaca atacccggct gaatcccgac aatcccgatc
     4141 cctcggccga agtcgtgtgg ggccagcaga caaccgacga gatgttcagc acccgcttca
     4201 agtacagtct ggcgaaaaag cccgccggcc aggagcacta gacatgcgat ggttggcaaa
     4261 tctcgggctg gctttgctgc tggcgtcgat tgtgccgctg gcggccgatg gccgctcgcc
     4321 ccagccggct gcggtcgtgc aggcgcttac gcaagccgcc cagaacgcac cgcagccgca
     4381 ggagtcggtc ggtgaggccc tcggacgact gaagaactcc ctgcaggagc tggatgcggt
     4441 cttgctccgc aatgacgagg cgatccgccg cggctggagc gagtatctcg acctgccggc
     4501 gattcaggcc gagctgacca gaccgcagcc cgatacggct cttttggaag ccaggtccaa
     4561 gcggttctat cagaaccacg tcggcctgga gcggtctcaa ttcattgacg tccgtagcga
     4621 gctgcgcggc tatctgatcg cccgcgagta cgcggttatg gaccagccgc tcgaggaata
     4681 tcggcagcgt gtaaccgagc tttccgattg cctggcccgc atcgacgcct cgttcgacga
     4741 agccgacgcc caccgcgcgg gcgtgctggc caactggctg gctccgctga gcgacgaggg
     4801 ggcgaacctg gcggatgctg tccgcgagaa ttattgccgc accaatggcg tcggccatgt
     4861 ctcctcgcgg gcgatcaatc tgctcatgga gcggagcgtg aaggagcaga actacattca
     4921 agaaacgatg atgggcgcct tgacgcgggg caccgcctat atgaacggcc aggtgacaat
     4981 ggccgtcgtc ccgaattcaa cgcaagcgtc actcgaaatt cgtctcgccg gccaggtcag
     5041 tgttccttcc accgtttcgc agcgcggcca gatcactgcg tatagttcct cgaactccgg
     5101 cctgtatgcg accaagcagg tgaatatcga caggaacggc ctctcgctcc gtcccgccgc
     5161 aacggccagc aacacgaaca tgcagattca ggacgtcgtc gccccctcgc ggatcatcga
     5221 gcgtctggcc tggcggcggg cgaaccggat gctgcccgag gcccagacgg ccgccaatcg
     5281 ccgcacccag acggaaatca acacgaacct cgacgagcag ggggccgcaa tgctctcggg
     5341 cgtgaacgac accttcctca acaaggtctg ctggcccatg ttgcgggcga acaccttgcc
     5401 cccggaaatg cggttctgga ccgacagcac ccatctgcgg ctggcatttt cgcagtggaa
     5461 cgatcggcaa ctggccccgg ccagccccgt cccgcagctt ctggacgaat acgacatggc
     5521 ctgcggggca cacgaatcga tgatcaacaa cttcaccgag gtgctgcttg gtgggctaac
     5581 cagcaaggac gaaggttggg cccacgtcat ggaaatcatg accggctcgg tgccccggcc
     5641 cttgtgggtc cacgaccggg ccccacgctg gaccgcgacg tttgaaaaag agcgaccctt
     5701 cattgcccac tttgacggcg ggcggtgcag ctttaccctg cggctgacga aggtgacgca
     5761 gggcgacaaa gtgtacgagc acccggtcga ggtcgaagcc cacatgatcc cgcggatcac
     5821 taaggacggg ccggcgctct ttcgcgacgg cgacctggac atccgcgtcg cccccggcat
     5881 gcagtcgcaa gccgaagccg agctgcggaa actgctggcc cgaaaagtcg gcgccgtctt
     5941 cccgccggaa ctctatttcg acggcctcgc gccacctgcc ggcggcacta ttgggcggct
     6001 ccgccaggtg cagatctccg aaatgcgctg cgaggatggc tggatgaccc tcggctacaa
     6061 gctgcaaacc gccccggtcg aagtcgcggg cggcggatcc taacggtccg cgtttgggca
     6121 tgaatcgctg gcgttacgac gacagtgctt tccacacctt cggcggcgac atgggtagct
     6181 cggtgagccg cttgcccgtg gcggcatgga tcgcgttggc gacggcagcg gggggcggaa
     6241 caatcggcgt ctcgccgacg ccgcggacgc cgaaggggtg cgaggggttc ggcacttcga
     6301 cgataatcgt ctcgatcatt ggcagatcga ggcaggtcgg catgcggtag tcgaggaagc
     6361 tcgcgtttcg catccggtcc tccttgtcga gccagtattc ctcattcagc gcccagccga
     6421 tgccctgcgc cgcgccgcct tgcatctggc cttcgacata gctcggatgc acggctttgc
     6481 cggcgtcttg aatggccgtg tagcggagga tcgtcacctt gccggtttcg ggatcgacct
     6541 ccacgtcgca gatatgcgtg ccaaagccgt tggtggaacc ttcgggggcg acgctcgcct
     6601 ggccgacgac tggcggcagg ttcttctgcg agatcttcgc ggccagttcc ttaaacgcaa
     6661 gcctgttgcc gttgttgctg tatgtgccgc cgacaacctc cacttcgtcg ggcttgcagt
     6721 cccacagttt cgcggcccgc tctcgcatct cttcctgcag caccag
//
LOCUS       DASUGB010000899         4324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_935127, whole genome shotgun sequence.
ACCESSION   DASUGB010000899 DASUGB010000000
VERSION     DASUGB010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935127"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1023
                     /locus_tag="VFV87_17090"
     CDS             31..1023
                     /locus_tag="VFV87_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5105539.1"
                     /translation="MSDDRAARRWVGFDLGGTKMLAAAFSSDFRALGRKRRKTKGSEG
                     VKAGLDRIVETINDALEEAQIKPNQLSGIGVGCPGNIDLEKGTLIDSANLGWKNFPIR
                     DALEKTFGCPVVVLNDVDAGVYGEYRFGAGKNARCVVGVFPGTGIGGACVYEGKIIRG
                     KNMSCFEIGHVQVNPQGIPCGCGRIGCLETEASRLAISAAAAMAAFRGEAPHLLSVAG
                     TDLANIRSGVLADAIRAGDAIIEQIVQRAARLIGWAVGDVVNLLLPDVIVLGGGLVEA
                     MPGIFVEGVEEAARQRAAPPFAKSFKVAPAKLGDDAVVRGAAAWAEATTATGGD"
     gene            complement(1048..1953)
                     /locus_tag="VFV87_17095"
     CDS             complement(1048..1953)
                     /locus_tag="VFV87_17095"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5105540.1"
                     /translation="MFRSLSFAAVLVACASAATASAADPPTDDGKLRIVVFGAHPDDA
                     EYKAGGCGAKWAKLGHHVLLCSVTNGDIGHWKMAGGPLAERRTAEVAACSKILGTTSR
                     VLDIHDGELEPTLENRKKIIRVIREWKADIVIAHRPWDYHPDHRYVGVLVQDAAYMVT
                     VPFICPDTPVLTKNPVFLYSSDAFKKPYPFDADVAISIDDVFDQKLEAIHELESQHYE
                     GGANGSEAHVRSVPPASDVAGRKAWLREKWEQRQSNEANRFRAALTERYGEEQGKEVK
                     YAEAFEICEYGRQPSREELKKLFPF"
     gene            2061..3245
                     /locus_tag="VFV87_17100"
     CDS             2061..3245
                     /locus_tag="VFV87_17100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105541.1"
                     /translation="MNEPKTPLPRNVVLLGWASLLNDVASEMLYPLLPTFLITVLGGN
                     KAWLGIIDGLAETASSLLKLWSGARSDVAASRKRFVIAGYGLAAIARPLIGLAGYPWQ
                     MLSLRVLDRVGKGIRTAPRDALIAEAAGERTRGRAFGFHRAMDHLGAAIGPLMAFGFL
                     WLWPGQLRLLFLLAILPAIPILILVAFGVREGRRASPATTHSELKLSLKPFDGRFRLY
                     LVALAIFTLGNSSDGFLLVRAGELGVPTAWLPVLWGVFHIAKSGLSYAAGPWIDRLGA
                     RPLIWAGWLIYAATYLAFAFATSAWHAWALFLVYAAFYALTEPSEKALVAGLVGEEHR
                     GLAYGWFNLTIGIGALPASLAFGFIYQYLGALTAFGMGAALALVAALLLIGVRERSKP
                     AS"
     gene            complement(3253..>4324)
                     /locus_tag="VFV87_17105"
     CDS             complement(3253..>4324)
                     /locus_tag="VFV87_17105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5105542.1"
                     /translation="TADKQYIGSPSIAVLPNGSYVASHDFFSPGNMGDRTHVFRSTDR
                     GQSWTKAAELTGQWWSGLFVHRGKLYLMGTSKAYGACIIRRSDDGGSTWTEPKVEASG
                     VILGAGKYHTSSMPVVVHNGRLWRAMEDGDGPGGWGSHFRAFMLSVPEDADLLVAKNW
                     TASSRLGRDPAWLDGKFGGWLEGNAVVTPEGGIVDILRVDTKTQPEFAAMIEISDDGK
                     KATFDPAQGFIEFPGGAKKFNIRYDAPSKHYWSLVNIALPQHASEVAPRVRNAVALVR
                     SPDLRKWETRCILFYHPDVKFHGFQYADWLIEEGDIIAAIRTAFDEPAGQAHNQHDAN
                     YLTFHRWKNFRELTMGDSVVKP"
BASE COUNT          722 a         1349 c         1404 g          849 t
ORIGIN      
        1 gaccgcctcc atttgctaag atttggcccc atgagcgacg accgcgctgc acgacgttgg
       61 gtgggcttcg atctgggggg cacgaagatg ctagccgcgg ccttcagcag cgatttccgc
      121 gcgctgggac gtaagcgccg caagaccaag ggctcggaag gcgtcaaagc cggtctggac
      181 cggatcgtcg agacgatcaa cgacgccctg gaagaggcgc agatcaaacc caatcagctt
      241 agcggtatcg gcgtgggttg cccgggcaat atcgacctgg aaaaagggac cctgatcgac
      301 tcggccaatc tcggctggaa gaattttccg attcgggacg ccctcgaaaa aacgtttggc
      361 tgcccggtgg tcgtgctcaa cgacgtcgat gcgggcgttt atggcgagta ccgcttcgga
      421 gccggcaaga acgcccgctg cgtcgtcggt gtctttccag gcaccggcat cggcggcgcc
      481 tgtgtctatg agggaaagat cattcgcggc aagaacatgt cgtgcttcga gattggccac
      541 gtgcaggtca atccacaagg cattccgtgc ggctgcgggc ggattggctg cctggagacg
      601 gaggccagcc gcctggccat ttccgccgcc gcggccatgg ccgccttccg cggcgaagca
      661 ccgcacctgc tttcggtcgc aggcacggac ctcgccaaca tccgcagcgg cgttttggcg
      721 gacgcgattc gcgcgggcga cgcgattatc gagcaaattg tacagcgagc cgcccgattg
      781 atcggctggg cggtggggga cgtagtcaat ctgcttttgc ccgacgtgat cgtcctcggc
      841 ggcggcctgg tcgaagcgat gcctgggatc tttgtggaag gcgtcgagga agcggctcgc
      901 cagcgggccg ccccgccgtt cgcaaaatcg ttcaaggtcg ccccggctaa actgggcgat
      961 gatgccgtcg tccgcggcgc ggcggcctgg gcggaggcga cgacagcgac aggcggcgat
     1021 tgagccgttc ggctcggccg cagcccatca gaacggaaat agcttcttca gctcctcgcg
     1081 cgaaggctgg cggccgtatt cgcagatttc gaaggcttcg gcgtatttga cctccttgcc
     1141 ttgctcctcg ccgtaccgtt cggtcagagc ggcgcggaag cggttagcct cattgctctg
     1201 ccgctgctcc cacttctcgc ggagccaggc tttgcggccg gcgacgtcgc tggccggcgg
     1261 caccgagcgg acgtgggctt cgctcccgtt ggcgccccct tcatagtgct gcgactccag
     1321 ctcatgaatc gcttccagct tctgatcgaa cacgtcgtcg atcgaaatcg ctacgtccgc
     1381 gtcgaacgga tacggcttct tgaaagcgtc gctggagtac aggaagacgg ggttcttcgt
     1441 gagcaccggc gtgtccgggc agatgaaggg aacggtcacc atgtaagcgg catcctggac
     1501 cagcacgccg acgtagcggt ggtcgggatg atagtcccac ggccggtggg cgatcacgat
     1561 gtcggctttc cactcgcgga tgacgcgaat gatcttcttg cggttttcga gcgtcggctc
     1621 cagttcgccg tcgtggatgt cgagcaccct ggaggtggtg cccaggattt tggagcaagc
     1681 ggcaacttcc gccgtccgcc gctcggccaa tgggccgccg gccattttcc agtgtccgat
     1741 gtcgccgttg gtcaccgagc acaagagcac gtgatgcccg agcttcgccc acttcgcccc
     1801 gcagccgccg gccttgtatt ccgcatcgtc cgggtgggcg ccgaagacga cgatccgcag
     1861 cttgccgtcg tcggtgggcg ggtcggcggc actcgcagtc gcggcgctag cgcaagcgac
     1921 gaggacagcg gcaaaagaga gggagcgaaa catggcgcgg ctcctgtagc agtagtgttg
     1981 gaatgtgcgt gagttcgaag tacggagtat aaatcaccaa ctcgtgcagc tctcgcaata
     2041 ctccggaatt tgcgattctc ttgaacgaac ctaagacacc gctgccgcga aatgtcgtcc
     2101 tgcttggctg ggcgagtcta ctcaacgacg tcgcgagcga gatgctctat ccgctcttgc
     2161 cgacgttctt gattaccgtt ctcggcggga acaaagcctg gctggggatc atcgacggcc
     2221 tggccgaaac tgccagcagc ctgctcaaac tctggtcggg cgcgcggtcg gacgtggcag
     2281 ccagccgcaa gagattcgtc attgccggct acggcctggc ggcaatcgcc cggccgctga
     2341 tcggcctggc cggctatccg tggcaaatgc tttccctgcg cgtgcttgac cgggtcggca
     2401 aaggaattcg caccgctccg cgcgacgcgc tgatcgccga agcggcaggt gaacggactc
     2461 gcgggcgagc ctttggcttt caccgggcga tggaccatct tggcgcggcg atcggcccgc
     2521 tgatggcgtt tggatttctc tggctttggc ccggacaatt gcgcctgctg tttctgctgg
     2581 caatcttacc ggcgattccc atcctgattc tcgttgcatt tggtgtgcgc gaagggcgcc
     2641 gggcctcgcc agcaaccacc cacagcgagt tgaagctctc gctaaagccg ttcgacggcc
     2701 gcttccgcct gtatcttgtc gcgttggcga ttttcacgct gggcaactcc agcgacggct
     2761 tcctcctggt gcgagcgggg gaactgggcg tgccaacagc ctggctgccc gtgctctggg
     2821 gcgtgtttca catcgcaaaa agcgggctga gctatgccgc cggaccgtgg atcgaccgtc
     2881 tcggcgcacg gccgctgatc tgggccggat ggctgatcta tgcggccacg tatctcgcct
     2941 ttgccttcgc gaccagcgcc tggcacgcct gggcattgtt cctggtgtat gccgccttct
     3001 atgccctgac cgagccgtcc gagaaagccc tggtggccgg attggtcggc gaagaacacc
     3061 gcggcctggc ctacggctgg ttcaacctga cgatcggcat cggggcgctg ccggcaagcc
     3121 tggcttttgg ctttatctat caatacctgg gagcgttaac agcgtttgga atgggggccg
     3181 cgctggcgct cgtcgcggct ttgctgctca tcggcgtgcg cgagcgtagc aagcctgctt
     3241 catgaaaagt catcagggtt tcaccaccga atcgcccatc gtcagctcgc gaaagttctt
     3301 ccagcggtgg aaggtgaggt agttcgcgtc gtgctggttg tgggcctggc cggcgggctc
     3361 gtcgaaggcg gtgcggatgg cagcgatgat gtccccctct tcaatcagcc agtcggcgta
     3421 ctgaaagccg tggaacttga cgtccggatg atagaacagg atgcaccgcg tctcccattt
     3481 ccgcagatcg ggcgaacgga cgagggccac ggcgttgcgg acgcgcgggg cgacttcgct
     3541 cgcatgctgc ggcagcgcga tattgaccag cgaccagtag tgtttgctcg gcgcgtcgta
     3601 gcggatgttg aacttcttcg cgccgcccgg gaattcgatg aatccctgcg ccggatcaaa
     3661 cgtggccttc tttccatcgt cgctgatttc aatcatcgcc gcaaattccg gctgggtctt
     3721 cgtatcgacg cggaggatgt cgacgatgcc tccctcgggc gtcacgacgg cattgccttc
     3781 cagccagccg ccgaatttgc cgtccagcca tgccggatcg cggcccaggc ggctgctagc
     3841 ggtccagttc ttcgcgacga gcaaatcggc gtcctccggc accgagagca tgaacgcccg
     3901 gaagtgcgat ccccagccgc cgggaccgtc gccgtcctcc atcgctcgcc agaggcggcc
     3961 gttgtggacg acgaccggca tcgacgacgt gtggtatttg cctgcgccca ggatcacacc
     4021 actcgcttcg accttcggct ccgtccaggt gctgccaccg tcgtcggagc ggcgaatgat
     4081 gcacgcgccg taggccttgc tggtgcccat caggtacagc ttgccgcggt ggacgaacag
     4141 gcccgaccac cactgcccag tgagctccgc ggctttcgtc cacgactggc cgcgatcggt
     4201 cgagcggaaa acgtgcgtcc ggtcccccat attgcccggg ctgaagaagt cgtgcgaggc
     4261 gacatacgat ccattcggca gcacagcaat gctcggcgag ccgatgtatt gcttgtccgc
     4321 cgtc
//
LOCUS       DASUGB010000900         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_795554, whole genome shotgun sequence.
ACCESSION   DASUGB010000900 DASUGB010000000
VERSION     DASUGB010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795554"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFV87_17110"
     CDS             <1..437
                     /locus_tag="VFV87_17110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5105543.1"
                     /translation="LRYFNVYGPGESHKGKFASMIWQLSLQMKAGKRPRVFRAGEQKR
                     DFVSIEDVLHANLLAMKADHPPLAAVFNVGCGRAGTFNEVIAGLNAALKTNLEPEYFE
                     NPYAFFQNHTEADISETTRVLGYKPRYVNVGEGIGAYMAAVK"
     gene            526..1254
                     /locus_tag="VFV87_17115"
     CDS             526..1254
                     /locus_tag="VFV87_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX5105544.1"
                     /translation="MWIAVLAGGLASAGGETPPADGGGLIALKALLDEMRSVQTLSTS
                     FRCEKRMESLDVPLLSQGRLLVARREERVSMRLTTESPYFSELILADGKSRARSQHEA
                     SWSSSDQSSRPGLTAVMVLVAGCSVGELGNLADYFSVRPAETAAAPPSPPDPVPKTWF
                     VLEPTNKDMAIAVREVRLGFAGPGVKSAPGIRSLTDAVIVTAQGDVTHYEFFSPAFNA
                     ALPADAFRPVTLRPEGVFGAEVSP"
     gene            1251..1931
                     /locus_tag="VFV87_17120"
     CDS             1251..1931
                     /locus_tag="VFV87_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105545.1"
                     /translation="MIGTRRNFRSLLAGIALVGAAGPLASCSATPGGKPVSELSPEAA
                     RVAAALQRMPQPSRGEGITRTFFATFHAAGQRTTASGVLQYYGPRDFRITAATELGVL
                     LFDGRVDWAGVHVIRHMSGLDEGIVATLITDLSRAFDMPDDLEGLAASNDKMLLKRKR
                     GDGHTTTWTFDRSTGRLMRTDIDMGFLDTLHIEYRAYAAQGWPSDLYVQRRARLYDIS
                     LTFTKSQS"
     gene            complement(1932..>2564)
                     /locus_tag="VFV87_17125"
     CDS             complement(1932..>2564)
                     /locus_tag="VFV87_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105546.1"
                     /translation="RGTYMVPVSEGAAGAQQLVSTGGGVLSYPQEYRDLEPNIALLRN
                     LADASGGEYLNDVGNPFEPKPQPVKTFWPLWQLLLLLVTSALFFDIAWRRLNLSDWYR
                     PASTPVAAVTRTDATVGAMKTVKSGRRDVDTQRKTMRERVEALAATSAPASDPPAPLQ
                     NNTSDVSGPMTATKREAAPAPQPGEGYANRLMSAKRRAKEQIREQSKEDV"
BASE COUNT          425 a          801 c          859 g          479 t
ORIGIN      
        1 gccttcgcta cttcaacgtc tatggcccgg gcgagtccca caagggaaag ttcgccagca
       61 tgatctggca actgagcctg cagatgaaag ccggcaaacg cccgcgtgtt ttccgcgccg
      121 gcgagcagaa acgcgatttt gtctccatcg aagatgtcct ccacgcaaac ctgctggcca
      181 tgaaggccga tcacccgccg ctggccgcgg tcttcaacgt cggctgcggc agggcgggga
      241 cgttcaatga agtgattgcg ggtctgaatg ccgcgctcaa gacgaatctc gagccggagt
      301 attttgagaa cccctatgcc ttcttccaga accacacgga ggcggacatt tccgaaacca
      361 cgcgcgtgct cggttacaag ccaagatatg tgaacgtcgg cgagggaatc ggggcgtaca
      421 tggcggcggt gaagtgaagg cggaaacaac cacgcagtgg ctgcgactcg gcgaaggggt
      481 aacttggtga cgtgtgccag tcagaagaaa agggcggcgg cagcggtgtg gatcgccgtc
      541 ctggcgggcg ggttggccag cgcgggagga gagacgccac cggccgatgg cggcgggctt
      601 atcgccctga aggcgctgct ggacgagatg cgcagtgttc agacactctc cacctccttt
      661 cgctgcgaaa agcgcatgga gtcgctggat gtgccgctcc tgtcgcaggg gcgactgctc
      721 gtggcgcggc gcgaggagcg cgtgtcgatg cggctgacca ccgaatcgcc ctatttctcg
      781 gagctgattc ttgccgacgg caagtcgcgc gcgcggtcgc agcacgaggc ctcctggagc
      841 agctccgatc agtcgtcgcg ccccgggctg acggcagtga tggtgcttgt cgccggctgc
      901 tccgtgggag agctgggaaa tcttgccgac tatttctctg tccgcccggc ggagaccgcg
      961 gcggcccctc cctctccgcc cgatccggtc ccgaaaacct ggttcgtgct ggagcccacg
     1021 aacaaggaca tggcgatcgc cgtgcgcgag gtgcggcttg gctttgccgg cccgggcgtc
     1081 aaatccgcgc cgggtatccg cagcctcacg gacgccgtga ttgttacggc acagggagat
     1141 gtcacgcact acgaattttt cagccccgcc ttcaatgccg cacttcccgc cgacgccttt
     1201 aggccggtga cgctccgtcc tgagggtgtt tttggcgccg aggtgagccc atgatcggca
     1261 cgcgacgcaa ctttcgctcg ctgctcgcgg gtattgctct tgttggcgct gccggtccgc
     1321 tggcgtcgtg cagtgcaacc cccggtggca agccggtctc ggagctttcg ccggaagccg
     1381 cgcgcgttgc cgcggcactc cagcggatgc cccagccatc gcggggcgag ggaatcaccc
     1441 gcacgttctt cgccacgttt cacgcggccg gccagcggac gacggcatcg ggcgtgctgc
     1501 aatactacgg cccgcgcgat ttccgcatca ccgccgccac ggaactgggc gtgctcctct
     1561 tcgatggccg cgtggactgg gccggcgtcc acgtgatccg ccacatgagc ggcctcgatg
     1621 aaggaatcgt cgcgacgctg atcacggatc tctcgcgcgc tttcgacatg cccgatgacc
     1681 tggagggtct ggcggccagc aatgacaaga tgctccttaa gcggaagcgc ggcgacggcc
     1741 acacgaccac gtggactttc gaccgctcga ccgggcgcct gatgcgcacg gatatcgata
     1801 tggggttcct cgatacgctc catatcgagt atcgcgccta cgcggcccag gggtggccca
     1861 gcgaccttta cgtgcagcgc cgcgcacgcc tgtatgacat ttctttgacg tttacgaaat
     1921 cacaatcgtg attaaacgtc ctccttgctc tgttcgcgaa tctgctcttt ggcgcggcgt
     1981 ttggcgctca tcaggcggtt ggcgtagccc tcgcccggtt gcggcgcggg cgcggcctcg
     2041 cgtttcgtgg ctgtcatggg gccggaaacg tcggaggtgt tattttgcag gggcgcgggc
     2101 gggtcgctgg ctggcgcact ggtggccgcg agagcctcga cgcgttcgcg catggtcttg
     2161 cgctgggtgt cgacgtcgcg gcggccgctc ttgaccgtct tcatagcccc cacagtcgcg
     2221 tccgtgcggg tgaccgccgc caccggtgtg gacgcgggcc ggtaccagtc cgagagattc
     2281 agtcgccgcc acgcgatgtc aaagaacagt gcgctggtta caaggagcag cagaagctgc
     2341 cagagtggcc agaaggtctt cacgggttgc ggcttgggtt caaaggggtt ccccacgtca
     2401 ttcaggtact ctccaccgga cgcatcagcg aggttccgga gcagtgcgat gttcggctcg
     2461 aggtcccggt attcctgggg gtatgacagg acgccgccgc cggtggagac gagttgctga
     2521 gcgccggcag caccttcgga gacaggaacc atgtaggtgc cgcg
//
LOCUS       DASUGB010000901         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1073485, whole genome shotgun sequence.
ACCESSION   DASUGB010000901 DASUGB010000000
VERSION     DASUGB010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073485"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..522
                     /locus_tag="VFV87_17130"
     CDS             268..522
                     /locus_tag="VFV87_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105547.1"
                     /translation="MNNDSTSALEGIPGLLKMAGTLAIASAIPLGAGVAVQEIKHGGL
                     WAIVVCFSVIAAMSALGFFLIVTSGLITKRIERMRSDSEP"
     gene            824..>2902
                     /locus_tag="VFV87_17135"
     CDS             824..>2902
                     /locus_tag="VFV87_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5105548.1"
                     /translation="MITKRLVFAFAVLTSPAVALAQDKADKALPPEQAAAAWELPPGF
                     TAELFAGEPDIVQPMAFTFDDRGRVWVVECLSYPTWKEPGPGVTGSDRVTILEDTDGD
                     GRHDKRTVFYDQGLNVSGIEVGFGGVWLTAVPYLLFIPDKNADDKPDGEPQILLDGWD
                     LKARHNVVGNLAWGPDGWLYGCNGILSNSKIGKPGTPDNQRVEMNCGVWRYHPVRHQF
                     EAYAHGTTNPWGIDWDERGQMFITNCVIKHIFHVVPGAHFQRMFGQDINPHSYGLIES
                     CADHFHWAGGHWTSSRRGQEANYPAHSDAGGGHAHSGCMIYLGDNWPKEYRGDAFMVN
                     IHGQRLNRDHLKREGSGYVAQHGPDEFFSKDPWFRGVAVKYGPDGGVYVADWSDTGEC
                     HDHTEEECDKSGGRIFKIEYGEPKRREATDLATLADDQLVALQFQPNEWQARHCRRLL
                     QERAAKGGLNRDAAEPLFYHVVRPTTNRDFDDDTSLIYGLRALFTLHAINLLDDDTLT
                     RSLHSPFDVVRSAAMLIAAESRRKPVAALSTLALQDDSPFVRLHIASALQRLPLEERW
                     PIAAALLSHAEDADDHYLPLMDWYAVEPLVPHNVRKAIEMLPQVKIPLVRQYMTRRIV
                     AVYEDGGAEKGSAAWVLDELLRTLAGADDAVRGDMLRGLADVYRGRKSVVTPAAWNAC
                     YAALEESPNGL"
BASE COUNT          597 a          861 c          919 g          525 t
ORIGIN      
        1 caggaacttt tgatattttg tacccttgga tgcaataata ctgtccactt gcgcatctat
       61 aggaagcggg cgtttgcgcg cgacgatcgc cgctgctccg ggcaggtcca cggaagaaag
      121 ccgccgaacg gccaggccag atcatccagt tcagacggat agcggaagag cgcaaaaggt
      181 gcgagttggc ggccaaaaaa gccggtggaa agccctccga gtggattcgt gctactctta
      241 tagaagcggc agagaagggc caaggcaatg aataatgatt cgacaagtgc tctggaagga
      301 attcctggcc tgctaaaaat ggccggtacg ctggcgatcg cttcggccat tcctctgggt
      361 gctggcgtcg cagttcaaga aattaagcac ggtggcttat gggcgattgt tgtctgcttc
      421 tccgttattg cggccatgtc agcactcgga ttctttctca ttgtgaccag cgggctaatc
      481 acgaagcgca tagaacggat gcgaagtgat tcggagccgt agagccagct aactagcttg
      541 ggttggcaaa gtagcatgtt ccgtcgcctg aatatgcagg cagccgaatt gaggcgttgc
      601 ttcccgcgcg ggttgcgtcc agaatgtccc ccgcctgggg actgttcagc ggttcgcgga
      661 aaagtttagc gcgggctaaa ctcagccaga ggcatcgcat ttacaagtac tatcaacgca
      721 acgacttgca gccgagttga gcttcaaaca atcgaaaagc ttagcgtcaa tggcgatcgg
      781 ctgcgacata ttcaacggcc agtgacggaa ggaaatggct gaaatgatta caaagcgact
      841 ggtgtttgct tttgcggtcc tgacgagtcc ggcggtcgcg ctggctcagg acaaggccga
      901 caaggccctg ccgcccgaac aggccgcggc agcttgggag ctcccgccgg gattcaccgc
      961 ggagctcttt gccggcgagc cggatatcgt ccagccgatg gcgtttacgt tcgacgaccg
     1021 cgggcgggtg tgggtcgtcg aatgcctctc gtacccgacc tggaaggagc ccgggccggg
     1081 cgtcaccggc agcgaccgcg tgacgatcct ggaagacacc gatggcgacg gccgccacga
     1141 caagcggacg gtgttttacg accaggggct gaacgtcagt gggatcgagg tcggcttcgg
     1201 cggcgtgtgg ctgacggcgg tgccctacct gctattcatt ccggacaaga acgcggacga
     1261 caaacccgac ggcgagccgc aaatcctgct cgacggctgg gatctgaagg ccaggcacaa
     1321 cgtggtcggc aacctcgcct ggggccccga cggctggctg tatggctgca acggcattct
     1381 cagcaacagc aaaatcggca agccgggcac gccggacaac cagcgcgtgg agatgaactg
     1441 cggcgtgtgg cgctatcacc ccgtgcggca ccagttcgag gcctatgccc acggcacgac
     1501 caacccgtgg gggatcgact gggacgaacg cggccagatg ttcatcacga actgcgtcat
     1561 caagcacatt ttccacgtcg tgccgggcgc tcacttccag cgaatgttcg gccaggacat
     1621 caacccgcac agctacggcc tgatcgaaag ctgcgccgat cacttccatt gggccggcgg
     1681 acactggacc agttcgcggc gcgggcagga agccaattac cccgcccata gcgacgccgg
     1741 cggcggccac gcccacagcg gctgcatgat ctacctcggc gacaactggc ccaaggaata
     1801 tcgcggcgac gccttcatgg tcaacattca cggccagcgg ctcaatcgcg accacctaaa
     1861 gcgcgaaggg agcggctacg tcgctcagca cggcccggac gaattcttca gcaaagaccc
     1921 ctggttccgc ggcgtcgccg tcaaatacgg ccccgacggc ggcgtctatg tcgccgactg
     1981 gtccgacacc ggcgagtgtc acgaccatac ggaggaggaa tgcgataaga gtggcgggcg
     2041 gatcttcaag attgaatatg gcgaaccgaa gcgccgcgag gcgacggacc tggcgacgct
     2101 agccgatgac caactggtcg cactgcaatt tcagccgaac gagtggcagg ctcgccattg
     2161 ccgccgattg ctgcaggaga gggccgcgaa gggcggcctc aatcgagatg cggcagagcc
     2221 gctattctat catgtcgttc gacctactac gaatcgcgat tttgatgacg acacctctct
     2281 gatttacggc ctgcgcgcgc tattcactct ccacgcgatc aacttgctcg atgacgacac
     2341 tctaactcgg agcctccatt cgccattcga cgttgtacgt tcagccgcga tgttgatcgc
     2401 tgctgaatcg cggcgcaagc cagtggcagc gctatcgacg ctggcgcttc aggacgattc
     2461 acccttcgtg cgccttcaca tcgcttccgc cctgcagcgg ctgccgttgg aagagcggtg
     2521 gccgattgcc gctgcgctgc tatcgcacgc cgaggatgcc gacgatcact atctgccgct
     2581 gatggactgg tatgccgtcg agccgctggt gccgcataac gtgcgcaagg cgatcgagat
     2641 gctcccgcag gtgaagattc cgctggtgcg gcagtacatg acgcggcgga tcgttgccgt
     2701 ctatgaggat ggcggggcgg agaagggaag cgcggcctgg gtgctcgacg aactgttgcg
     2761 gacactggcc ggcgcggacg atgccgtgcg cggcgacatg ctccgcggcc tggccgacgt
     2821 ctatcgcggc cgcaagagcg tcgtcacgcc ggctgcgtgg aacgcctgct atgcggcgct
     2881 cgaagaaagc ccgaacgggc tg
//
LOCUS       DASUGB010000902         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1212406, whole genome shotgun sequence.
ACCESSION   DASUGB010000902 DASUGB010000000
VERSION     DASUGB010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212406"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VFV87_17140"
     CDS             complement(<1..1381)
                     /locus_tag="VFV87_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105549.1"
                     /translation="MSHADPMTEVLANYRQQEATDFGQAWVGMEPTFQSEKSVRKWIK
                     MSAEPGGEDAYFEDDYMLKTQKKVVKKIKKRYEEQREEGQPHCMFARVELDDDLDQWQ
                     VRRQNLRFFFGEDEGLEPLEVRLSLDPETFEYSIKPVPLAWFYDERFVTFLEEFLWKT
                     PRKLGLSFAIAHGGGQFSLSAKTVMTGSLLADDIAARLNHPELATWIMDWPNPDDRAF
                     RATGRRKAAFQEVLSAYWAGRFHPRALGALTAENALLDRGFGPACVAPEGLMESGRGP
                     VGDAREVFQTNFAFGRTVRWNAQNVDPGYWQSAHPDEDGYRPDQIMRYSEGNLNRLQI
                     AGELHVKSGKVLDPEQAPEMDAPLELALLTTEASWENRAQMSRTSARDYVEAQLLYVH
                     SLRRLQQHPHVAVTESLLQDQILGDAEQTLRHHGGTATLDQLRRAARQINLDESRGRL
                     NTDWIEPETL"
     gene            complement(1441..2478)
                     /locus_tag="VFV87_17145"
     CDS             complement(1441..2478)
                     /locus_tag="VFV87_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HEX5105550.1"
                     /translation="MREDVEIIPADEADDIQRVVQALEILLRRSQVKTGQFRADVHVK
                     THGYAQGEFRVLPNLPNELAQGLFEHAGGYPAVVRFSNAASQPQPDAIPDGRGMSIKV
                     LEVAGDLVQTDEQSGPAQDFVMINHPVFFARNVKDYLRLEQVLVQADDNSLATLQGAL
                     TGGDWNPLHWHWREMLTAARIAGQLPAHPASNTYFSMAPIRFGDYVAKYRAKAAGDRL
                     DSYLDLVQRLGSEADALRLALEEMLHTQEVLFEFQVQLRTSAQTMPIEDATVEWPESE
                     SPYRTVAHLLLPRQEIAALRQQSAYQNLAFNVWHALAVHRPLGGINRVRRWAYALSSA
                     WRRQPAAPKSE"
     gene            complement(2541..3353)
                     /locus_tag="VFV87_17150"
     CDS             complement(2541..3353)
                     /locus_tag="VFV87_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5105551.1"
                     /translation="MLIRLAMRLCEVSPLFKRFLWRQWYQYLAGYQRADWTFMNYGFT
                     SLTSDGPALELQPDDEPNRYSIQLYHHVASAVPLAGLDVLEVGSGRGGGASFVKRYHR
                     PEQLTGVDLSPKAVRFCRDQYRLEGLSFVPGDAESLPLDDKSFDAVLNVESSHCYGSM
                     PKFLEQVRRVLRPGGHFLFADLRSAADRDRLHRDIEECGMILLEQEDISANVLAALRC
                     ESARKLALIEHAVRQPMQNAFRQFAAVEGSEVYDGFKMGATVYLRYLLQRAK"
BASE COUNT          681 a         1082 c         1068 g          694 t
ORIGIN      
        1 acagtgtctc gggttcgatc cagtcagtat tcaatcgccc gcgcgattcg tcgagattga
       61 tctggcgggc cgcgcggcgc aactggtcga gcgtcgccgt gccgccgtgg tgccggagcg
      121 tttgttcggc gtcgccaaga atctggtctt gcaacagcga ctcagtgact gcgacgtgcg
      181 gatgttgctg aagacgccgc aaggaatgca cgtacagcaa ttgggcctcg acgtagtcgc
      241 gagcgctggt gcggctcatc tgggcgcgat tttcccaact ggcttcggtg gtcagcaggg
      301 ccaactctaa aggggcgtcc atctcgggcg cctgttccgg gtccaagacc ttgccgcttt
      361 tgacgtgcaa ttcgccggcg atttgcagac gattgagatt gccttcgctg tagcgcatga
      421 tttggtcggg gcgatagccg tcctcatcgg gatgcgccga ctgccaatag ccgggatcga
      481 cgttctgggc gttccagcgc accgtccgcc caaaggcgaa gttggtctga aacacctcgc
      541 gggcgtcccc cacaggaccg cgtccggact ccatcagccc ctcgggcgct acacacgccg
      601 gtccaaaccc gcggtcgagc aaagcgtttt cggcggtcaa tgcgccaaga gcgcggggat
      661 ggaaccggcc agcccagtac gcgctcaaga cctcctgaaa cgcggctttg cgtcgccctg
      721 tggcgcggaa ggcgcgatcg tcggggttgg gccagtccat gatccaggtg gccaactcgg
      781 gatggttcaa cctggcggcg atgtcgtcgg ccaacaggct tccggtcatg accgtcttgg
      841 cggagaggga aaactgtccc ccgccgtgtg caatggcgaa cgacagcccg agtttgcgcg
      901 gcgttttcca caagaattct tcgaggaacg tcacgaaccg ctcgtcgtag aaccaagcca
      961 gcgggacggg cttgatgctg tactcgaagg tctcagggtc gagcgacagc cggacctcca
     1021 gcggctccag cccctcgtcc tccccaaaaa agaagcgcaa gttttgccgc cgcacctgcc
     1081 attgatcgag gtcgtcgtcc aactccactc gcgcgaacat gcagtggggc tgcccctcct
     1141 cccgctgctc ctcgtacctc tttttgatct tcttgacgac tttcttctgg gtcttgagca
     1201 tgtagtcgtc ttcgaaatac gcgtcctcgc cgcccggctc agcggacatc ttgatccact
     1261 tgcgaaccga cttctcactc tgaaaggtcg gctccatccc cacccacgcc tgaccaaagt
     1321 cggttgcctc ctgttggcga taattggcga gcacctccgt catgggatcg gcatgagaca
     1381 tagtggcagg atggaagact ttttttgcaa tatgagaaga agtgaaccag ccgtcaccgg
     1441 ctattctgac ttcggcgcgg ctggctgacg acgccaggcg gacgagaggg cgtaggccca
     1501 gcggcggacg cggttgatgc ctccgagggg acgatgtacc gcgagtgcgt gccacacatt
     1561 gaacgcgagg ttctggtaag cagattgctg ccgcagcgct gcgatctcct gccgcggaag
     1621 caacaggtgg gcgaccgtac gatacgggga ttcgctttca ggccactcca cggtggcgtc
     1681 ttcgatcggc atcgtttgtg cggaggtgcg cagttgcacc tgaaactcga acagcacctc
     1741 ctgcgtatgc agcatttctt caagcgccaa gcgcagcgcg tcggcctcgg accccaatcg
     1801 ctggaccaaa tccaaatacg aatccagtcg atctccggct gccttggccc ggtatttggc
     1861 cacatagtct ccaaaccgaa tcggagccat gctgaagtac gtgttgctcg ccgggtgcgc
     1921 ggggagttgt cccgcgatgc gagcagcggt cagcatctcg cgccagtgcc agtgcagcgg
     1981 gttccaatcc cctccagtca gggcgccttg caacgtcgcg agcgagttgt cgtccgcttg
     2041 cacgagaacc tgttccaagc gcaggtagtc cttcacgttc cgggcgaaaa acaccggatg
     2101 attgatcatc acaaagtcct gtgccgggcc gctctgctcg tcggtctgca ccaggtcgcc
     2161 tgccacctcg agcaccttga tggacattcc gcgaccgtcg ggaatcgcat cgggttgggg
     2221 ctggctggct gcgttggaaa accgaactac tgccgggtag ccgccagcgt gctcaaacag
     2281 cccctgggca agttcattcg gcagattcgg cagcacccgg aattccccct gggcgtagcc
     2341 gtgcgttttg acatgcacgt ccgcacggaa ttgtccagtc ttcacctgac tccgcctgag
     2401 taggatctcc aaggcttgga ccacccgctg tatatcgtcg gcctcgtcgg ccgggatgat
     2461 ttcaacgtct tctcgcatgg tgtattagac ggttggcaat ggtcattgga ggcggtttga
     2521 tggtcgtgcc tgggcagcgg ttacttcgcc ctctgcagga gataacggag atagacggtc
     2581 gctcccatct tgaatccgtc atagacttcc gacccttcga cggccgcgaa ttggcgaaag
     2641 gcgttttgca tgggttgcct gaccgcatgt tcgatgagcg ccagcttgcg cgcactttcg
     2701 cagcggagcg cggcgagcac gttggcggaa atgtcctcct gctccagcag aatcattccg
     2761 cactcctcga tgtcccggtg caagcgatcg cgatccgcgg cgctgcgcaa gtcggcaaag
     2821 agaaaatgcc caccagggcg caacacgcgg cgaacctgct ccagaaattt tggcatcgat
     2881 ccgtagcaat gcgacgattc gacattcaag acggcatcga acgacttgtc gtccagcggc
     2941 aacgactcgg catcgcccgg cacgaatgac aggccttcca ggcgatactg atcgcggcaa
     3001 aagcgaacgg ctttgggtga aagatcgaca ccggtcagct gctcgggccg gtggtagcgt
     3061 ttgacaaacg aggccccgcc gccgcggcca gagccgacct ccaagacgtc gagacccgcc
     3121 agcggaaccg cgctggccac gtggtgatac agctgaatgg aatatcgatt aggttcgtcg
     3181 tccggctgca actccaaggc cggcccatca ctcgtgaggg aggtgaagcc atagttcatg
     3241 aacgtccagt cggcccgctg gtagcccgcc aggtactgat accactggcg ccacaagaac
     3301 ctcttgaata gcggcgaaac ttcacagagc cgcatggcca ggcgaatcag catgacaacc
     3361 tcaggcctcg gatcgtttgg acttgacccg ccgccgatgg tgccagcgcc tcggcgcctc
     3421 gctccgccca ggccacgtaa aaaacagata atcggtcaac gggaaaacgg tgccgtaatt
     3481 cgcgttggcg tggacgtggt gcctgaggtg gtgattgcga aagaa
//
LOCUS       DASUGB010000903         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_378979, whole genome shotgun sequence.
ACCESSION   DASUGB010000903 DASUGB010000000
VERSION     DASUGB010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378979"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFV87_17155"
     CDS             complement(<1..880)
                     /locus_tag="VFV87_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105552.1"
                     /translation="MPAPTLFPTELTVADRQPKTDRLVLKDLLNRAAGDKRLEEPVLR
                     AAHKVLIKWAELDSAKKLTTLNETQMQGDFLAEVFGQALGYAGATDGTEVFHRQQHHE
                     IAGETPDAVLGFFQHDQPLDPLAVVELKGPKVHLDRDRSNGRTAVGQCWDYLINSPPT
                     CRWGIVSNYVSFRLYERSSTKQAYEHFKLQDLRNFDEFRRFYALFHRQGLIDKYLGGL
                     PRAVALLKESSEQQRKVGDELYDNYSRNRLALIEVLHFKEKHPLHLAIEMAQRLFDRI
                     MFIAFCEDRHLLPEKTI"
     gene            1194..>2213
                     /locus_tag="VFV87_17160"
     CDS             1194..>2213
                     /locus_tag="VFV87_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5105553.1"
                     /translation="MLAKLFTFALSGIEALPVEVEVDVSAGALPKTTLVGLPEAAVKE
                     STHRVERAMANSGFVRPKDRIVINLAPAELPKHAASFDLPISLGMLAGSGQLQSELFE
                     QYAVVGELALEGTTRPVKGALSMAMAAARQKGLRGLVVPAESAAEAAVVQEVEVIPVA
                     SLTQAVGFFTGSLAIEPTPSQIEQLFEQLARYDDDFGDVRGQEMAKRAIVVAAAGSHN
                     ILMLGPPGSGKTMLAKRVPTILPALTAGESIETTRIYSSVGRLLPGQPLMARRPYRSP
                     HHTISEPGLVGGGNPPVPGEISLSHNGVLFLDELPEFNRRTLEVLRQPLEDGTVTISR
                     ALNSTT"
BASE COUNT          353 a          696 c          765 g          399 t
ORIGIN      
        1 ggatcgtctt ctccggcagg aggtgcctgt cttcacagaa ggcgatgaac atgatccggt
       61 cgaagagccg ctgggccatc tcgatggcca ggtggagcgg gtgcttctcc ttgaaatgca
      121 gcacctcgat cagggccagg cggttgcggg agtaattgtc gtacagctcg tcgccaacct
      181 tccgctgctg ctcgctcgac tccttgagca gggcgactgc acggggcagg ccgccgagat
      241 acttgtcgat caggccttgg cggtggaaga gggcgtagaa ccggcggaat tcatcgaagt
      301 tgcggaggtc ctgcagcttg aagtgctcgt aggcctgctt ggtgctggag cgctcgtaga
      361 ggcggaagct gacgtagttg gagacgatgc cccagcggca ggtcggcggc gagttgatca
      421 gatagtccca gcactggccg acggcggtgc ggccattgct ccgatcgcgg tccaagtgga
      481 ccttggggcc tttcagctcg accacggcca gcggatccag cggctggtcg tgttggaaga
      541 accccagcac ggcatcgggc gtttcaccgg cgatttcgtg atgttgctgg cgatgaaaaa
      601 cttcggtccc atcggtcgcg ccggcgtagc ccagcgcctg gccgaacact tcggccagga
      661 agtcgccctg catttgcgtt tcgttcagcg tggtcagctt cttggcgctg tcgagctcgg
      721 cccatttgat gagcaccttg tgggcggctc ggaggacggg ctcttccagg cgcttatcgc
      781 ccgccgccct gttgagcagg tccttgagga ccaggcggtc ggttttcggc tgacggtcgg
      841 cgaccgtcaa ttcggttggg aagagcgtgg gcgcgggcat ggtccgagga tgtcgtcctg
      901 acggctcggg gaggaaagac ttgggatgcg cagcggtcgc aattttttac ccaatttgaa
      961 ctacctgtga cacggggttg ccaaccgtgg gggcgtcgtg gacgaccctg atccgtcaaa
     1021 gaggtgaggt ccggcccctc actcctgacc tctgacctct gatctctgat ctctgacctc
     1081 tgccctctgc cctccgaacc ctgagccgca acttgcgatg acgcattcgc tcggccacaa
     1141 tcggggcact tccttgccat tcgccgagac ggactttccg ccccgcacgc gccatgctcg
     1201 ccaagctgtt caccttcgcg ctgtcgggca tcgaggcgct gcccgtcgag gtggaagtgg
     1261 acgtgtcggc cggcgccctg cccaagacga cgctcgtcgg ccttcccgag gcggccgtca
     1321 aggagagcac ccatcgcgtc gagcgggcga tggccaattc cgggttcgtg cggcccaagg
     1381 accggatcgt catcaacctg gccccggccg agctgcccaa gcacgccgcg tcgttcgacc
     1441 tgccgatcag cctgggcatg ctcgccggca gcgggcagct gcagtcggaa ctgttcgagc
     1501 aatacgccgt ggtgggcgaa ctggcgctgg aagggacgac ccggcccgtg aaaggcgcgc
     1561 tgtcgatggc gatggccgcc gcccggcaga aggggctgcg ggggctggtc gtcccggccg
     1621 agagtgcggc tgaggcggcg gtcgtgcagg aggtggaggt gattcccgtg gccagcctga
     1681 cccaggcggt cggctttttc accggatcgc tggcgatcga gccgaccccc tcgcaaatcg
     1741 agcagctgtt tgagcagctg gcccggtacg acgatgactt cggcgacgtc cgcgggcagg
     1801 aaatggccaa gcgggcgatc gtcgtggcgg cggccggctc gcacaacatt ctcatgctgg
     1861 ggccgcccgg cagcggcaag acgatgctgg ccaagcgggt gcccacgatc ctgcccgcgc
     1921 tgacggccgg cgaatcgatc gagacgacgc ggatttacag ctcggtcggg cgattgctgc
     1981 ccgggcagcc gctgatggct cgccggccgt atcgctcgcc gcaccacacg atcagcgagc
     2041 cgggcctggt ggggggcggt aatccgccgg tgccgggcga aatttcgctc agccacaacg
     2101 gggtgctgtt cctcgacgag ctgcccgagt tcaaccgccg cacgctggag gtcctccgcc
     2161 agccgctgga ggacggcacc gtgaccatct cgcgggccct caacagcacg acg
//
LOCUS       DASUGB010000904         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_518873, whole genome shotgun sequence.
ACCESSION   DASUGB010000904 DASUGB010000000
VERSION     DASUGB010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518873"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFV87_17165"
     CDS             complement(<1..736)
                     /locus_tag="VFV87_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105554.1"
                     /translation="MKLGSFLLSACAVAGLASVALAQIPATQLTSIFPPGAKPGSTLE
                     VTVAGADMDDVEKLTFSHPGLTAAAKMSTPTDFEPTPKPVPGVFNLTIAGDVPPGQYE
                     VRAFGRFGISNARTFVVGSAEEIVEAGNNNSPENAQEVPVGSTLSGRVEQNTYDYLKL
                     NLKQGERVLIDLAAQRIDSRLDGTLVLLGSNGRELERVRDSIGTDPVLDFTAPAEGAY
                     LLKLQDAVYGGGGDYVYHLTVSASPFV"
     gene            complement(1016..>2485)
                     /locus_tag="VFV87_17170"
     CDS             complement(1016..>2485)
                     /locus_tag="VFV87_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEX5105555.1"
                     /translation="VDELVAKKLKALGLPASQIADDATFLRRVSIDLAGRLPTLEEAE
                     AFIADQAPNKRENLVNRLLDSTDYADYFANKWSAILRNKRRQDTEKLATFSFHEWIRQ
                     SLHENKPYDQFVREILTATGTPGQNPPVGWYREVKDSSAQLEDTAQLFLGLRIQCARC
                     HHHPFEKWSQQDYYGFSAFFSQVGRKKGYIQNEERIYHRRGVASASNPKTGQAVKPTG
                     LGAAPLELGPDQDPRHAMVDWMAAKDNPFFAKSLVNRYWKHFFGRGLVDPEDDMRVTN
                     PASNPELLETLAKDFVDHNFDLKHLCRTICNSTTYQLTAEPNEWNQDDKQNFSRYYPK
                     RLNAEVLLDAIDQVTGTQTGFGGIPAGTRAVQLPDNGFNSYFLTVFGRPEASSACECE
                     RSSEANLAQSLHLLNSSEIQGKLTAGNGHAARLANDKERPHELKVRELYLLAFARVPT
                     PEEMTIAIAHINKNEMDPKRAYEDIVWALINTKEFLFNH"
BASE COUNT          437 a          728 c          809 g          511 t
ORIGIN      
        1 cgacgaatgg cgacgcactc accgtcaagt gataaacata gtccccgccg ccgccataga
       61 cggcgtcctg cagcttgagc aggtaggccc cttcggccgg cgcggtgaaa tcgagcaccg
      121 gatcggtgcc gatgctgtcg cgcacccgct ccaactcccg gccgttggag ccgagcagca
      181 ccagcgtgcc atccagtcgc gaatcgatcc gctgggcggc gaggtcaatc agcacgcgct
      241 cgccctgctt gaggttcagt ttcagataat cgtaggtgtt ctgctcgacg cggccgctga
      301 gcgtcgagcc gaccggcacc tcctgggcgt tctccggcga gttgttgttg cctgcctcga
      361 caatttcctc cgccgagccg acgacgaacg tgcgggcgtt ggaaatcccg aatcgcccaa
      421 aagcccggac ctcgtactgt ccgggcggca cgtcgccggc gatcgtcaaa ttgaagacgc
      481 cgggcaccgg cttcggcgtc ggttcaaagt cggtcggcgt gctcatcttc gccgcggcgg
      541 ttaggcccgg atggctgaag gtcagctttt ccacatcgtc catgtcggcg ccggcgaccg
      601 tcacctccag agtggagcct ggcttggcac cgggcggaaa gatgctcgta agctgcgtgg
      661 cgggaatctg ggccagcgca accgaggcaa ggccagcgac cgcgcaggct gacaggagaa
      721 atgagccgag cttcatgact ggccgctcct gggagggaga ggagagttgg cgggtgggaa
      781 tctgtaggga acggcctctg tgccgttccg gatgcgtcgg tgtgcgattc gcggaacgcc
      841 gcggagggcg ttccctacag gttggttttc aggcgggagg tatcagttca gtctgattgg
      901 tgggtgaagc gtttccaata gccgggacca aacttggtcc cggagagttg ctagaagcac
      961 tgcctctggc ggcatggaag actcactccg ggacctaggt cccggctaaa agccgctaat
     1021 ggttgaacag gaactccttc gtattgatga gcgcccaaac gatgtcctcg taggcccgtt
     1081 tggggtccat ctcgttcttg ttgatgtggg cgatggcaat cgtcatttcc tcgggcgtcg
     1141 gcacgcgggc gaaggccagc aggtacagct cgcggacctt cagctcatgc ggccgctcct
     1201 tgtcgtttgc gaggcgagcc gcatggccat tgccggcggt cagcttgccc tgaatttcac
     1261 tcgaattgag caagtgcagg ctctgggcga gattcgcttc gctggagcgc tcgcattcgc
     1321 acgcgctcga agcttccggg cggccgaaga ccgtcagaaa gtacgagttg aaaccgttgt
     1381 cgggaagttg cacggcccgc gtgccggcgg gaatgccgcc gaaacccgtc tgtgtgccgg
     1441 tcacctggtc gatcgcgtcg agcagtactt cggcattcag gcgcttggga tagtagcgcg
     1501 agaagttctg tttgtcgtcc tgattccact cgttcggctc ggcagtgagc tggtacgtcg
     1561 tagagttgca gattgtgcgg cagaggtgct tgaggtcaaa gttgtgatcg acaaaatcct
     1621 tggccagggt ttcaagcagc tccggattgc tcgccggatt agtcacccgc atgtcgtctt
     1681 ccggatcgac caggccgcgg ccgaagaagt gcttccaata ccggttgacc agcgacttgg
     1741 cgaagaacgg gttgtcctta gccgccatcc aatcgaccat cgcgtgccgc ggatcctggt
     1801 cgggacccag ttccagcggc gcagcgccca ggcccgtcgg ctttacggcc tggccggtct
     1861 tgggattgct cgcgctggca acgccgcggc ggtgataaat ccgctcttcg ttctggatgt
     1921 atcccttctt gcggccgacc tgcgagaaga acgccgagaa gccgtaataa tcctgctggc
     1981 tccacttctc gaacggatga tggtggcagc gggcacactg aattcgcaag cccaggaaaa
     2041 gctgagccgt gtcttccaac tgggccgacg agtccttgac ttcgcgatac cagccgacgg
     2101 gcgggttctg cccaggcgtg ccggtcgcgg tgaggatttc gcgcacgaac tgatcgtacg
     2161 gcttgttctc gtggaggctc tggcgaatcc actcgtggaa cgaaaacgtt gcgagctttt
     2221 cggtgtcttg ccgccgcttg ttgcggagaa tggccgacca cttattcgcg aaatagtcgg
     2281 cgtaatccgt actgtcgagc agccgattga cgaggttctc gcgcttgttc ggggcctggt
     2341 cggcgatgaa cgcctcggct tcttcgagcg ttggcaatct gcccgcgagg tcgatcgata
     2401 cgcgtcggag gaacgtcgcg tcgtcggcga tttgcgaagc gggcaggccc aaagccttga
     2461 gtttcttggc taccagctca tcgac
//
LOCUS       DASUGB010000905         3776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_656193, whole genome shotgun sequence.
ACCESSION   DASUGB010000905 DASUGB010000000
VERSION     DASUGB010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656193"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFV87_17175"
     CDS             complement(<1..187)
                     /locus_tag="VFV87_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5105556.1"
                     /translation="MSVETARAALRSAKLRSTTSRVAVLQHLASASQPLSHAEMADAL
                     VPEGYDKSTLYRCLVELA"
     gene            complement(348..2321)
                     /locus_tag="VFV87_17180"
     CDS             complement(348..2321)
                     /locus_tag="VFV87_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5105557.1"
                     /translation="MNKINRRTAIQATAAGAGALALSPAALFAQEIAPQPFGTEFPNL
                     ESLTTGEWWTKGVAAAAGKAKGKAKAKAAAGGQPPPMDVPRSEVVAFAVYTHHDGVLK
                     ITAQLFPLKPGEERRAQLEFKRAGKWTPAGQAEVLYPGWDAHFRVEQWDNTQDVPYRV
                     RHGAEAVFEGLIRRDPRDKDEIVVANMSCNSSRTAGLRPEIIENLQRQNPDLLFFAGD
                     QTYRHTEHTAGWIEFGLQFRDILRDRPAICIPDDHDVGHGNLWGESGKRSTIPGDADG
                     GYRYPVEYVNQVQRQQSTHLPDPVDPAPVDRGITVYFTRLTVGGVDFAILEDRKFKTG
                     PAGKIPQMGPRPDHINDPNYDPKTVDVPGLELLGPRQEKFLETWSQDWTGAELKAVLS
                     QTAFCGAVHLHGNPNNRLLADLDCNGWPQTPRNRALALIRRAWAVHLCGDQHLAVVVK
                     HGIDEFNDGPYGFTSPALVNTIYGRWWHPLDEKPGPNPVAGSPLPWTGEFKDGLGNRM
                     SMLAYANPEDAGDEKKRADGYGLARFHKKSRTVTFECWPRFSDSTQGAMAQFPGWPIT
                     VAAEANDGRQVAGYLPELVFEEGVNPVVQVVEESSGEVLYTVRAHNSRFQPRVYSPGK
                     HAVKIGQNKPDSQTMAGLEPRPKEEAGQRTVKL"
     gene            2396..3433
                     /locus_tag="VFV87_17185"
     CDS             2396..3433
                     /locus_tag="VFV87_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5105558.1"
                     /translation="MLQIIAHGGDRRRAPPPRHPAHHESKKEPRADQVNSGHFYYDPL
                     FLEHETGRHPENAGRISALGSRVDDLVAQYRCTRGSWEPLSASDLTAVHDAAYVETVQ
                     RVAERGGGMLDPDTVVSPRSYEVARSAAGAVASAVDNVLAGEARRAFCLLRPPGHHAL
                     RDRGMGFCLFNNIAIGARRAIDKHGLARVLVVDWDVHHGNGTQELFWEDPQVGFFSIH
                     RYPFYPGTGAADETGGGAGLGTKLNVPVAYGTSRREYLDLFRGGLESLAEKMRPELIL
                     LSAGFDAHRLDPIGSLGLESEDFVTLTQSVVQIADSYSGGKIVSVLEGGYNPAAVAQS
                     VQLHLEQLFLD"
     gene            3594..>3776
                     /locus_tag="VFV87_17190"
     CDS             3594..>3776
                     /locus_tag="VFV87_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HEX5105559.1"
                     /translation="MTIDKSLRVKAGAIQNRNVLSRAERIAKLKEAERWKEGQSVLGL
                     PKVRVQKLALKKKKKAK"
BASE COUNT          680 a         1158 c         1208 g          730 t
ORIGIN      
        1 cagccaattc aaccaggcag cggtagaggg tcgatttgtc gtacccctcc ggcaccaggg
       61 cgtcggccat ttccgcgtgg ctcagcggct ggctggccga ggccaaatgc tgcagcaccg
      121 ccacccgcga cgtcgtgctg cgtaactttg cgctccgcaa cgccgcacgg gcggtttcga
      181 cagacattcc agccgaggtt cgactcacat caactccgtc gtcaaagccc atctgcctat
      241 tcaatgcgat tgcaatcgag ttgctgacgc ttgtccaatt tacgaccaca attcgccgcc
      301 agcaaggact ttcaagctcc cggcgacgcg ccacgcgcaa ggccgggcta caacttgaca
      361 gtccgttgcc ctgcttcttc cttgggccgt ggttccaggc cggccattgt ttggctgtcc
      421 ggcttgttct ggccgatttt gaccgcgtgc ttgcccggcg aatatacgcg gggctgaaag
      481 cggctgttgt gggcacgaac ggtatagagc acctcgccgc tgctttcttc gacaacttgc
      541 accacaggat tgaccccctc ctcgaacacg agttctggca ggtagcctgc cacctgtcgg
      601 ccgtcgttgg cttcggcagc caccgtaatc ggccagccgg ggaactgcgc catggctcct
      661 tgcgttgaat cgctgaaacg tggccagcat tcgaacgtca ccgtgcggct cttcttgtgg
      721 aagcgggcga ggccgtatcc gtcggcccgt tttttctcgt cgcctgcgtc ttcgggattg
      781 gcataggcca acatcgacat tcggttacca aggccatcct tgaattcgcc ggtccagggg
      841 agggggctgc cggcgaccgg attgggtccc ggcttttcgt cgagcgggtg ccaccagcgg
      901 ccgtaaattg tgttcaccag cgccggactg gtaaagccgt aaggaccatc gttgaattca
      961 tcgattccgt gcttcacgac gacagccaga tgctgatcgc cgcagaggtg aacggcccac
     1021 gcgcggcgga tcagtgccag cgcacgattc cgcggcgttt gcggccagcc gttgcaatcg
     1081 agatcggcga ggagcctgtt gttgggattt ccgtgcaggt gtacggctcc gcagaaggcg
     1141 gtctgggaga ggacggcttt caattccgcc cctgtccaat cctgcgacca ggtttcgagg
     1201 aacttctcct gccgcgggcc gagcagttcg aggccgggaa catcgacggt cttgggatcg
     1261 taattcgggt cattgatgtg gtcggggcgc ggccccatct gcgggatctt ccccgccggg
     1321 ccggtcttga acttgcggtc ctccagaatg gcgaaatcga cgccgccgac ggtgaggcgc
     1381 gtgaaataga cggtgatgcc gcgatcgacc ggcgccggat cgacggggtc cggcaagtgc
     1441 gtagattgtt gccgctgcac ctgattgacg tactccacgg gatagcgata gccgccgtcg
     1501 gcgtcgccgg gaatggtcga gcgcttgccg ctttcgcccc acaggttgcc gtggccgacg
     1561 tcgtgatcgt cgggaatgca gatggccggg cggtcgcgga ggatatcgcg aaactgcagt
     1621 ccgaactcga tccagccggc cgtgtgctcc gtgtgccgat aggtctgatc gcccgcaaag
     1681 aagagcaaat ctgggttttg ccgctgcaag ttctcgatga tctcaggccg taggccggcc
     1741 gtgcggctgg aattgcacga catgttggcg acaacgattt catccttgtc acgcggatcg
     1801 cggcggatga gaccctcgaa caccgcttct gcgccgtgcc gaacgcggta ggggacatcc
     1861 tgcgtgttgt cccactgctc cacgcgaaag tgcgcgtccc agccgggata gagaacctca
     1921 gcctggccgg ccggcgtcca tttgccggcc cgcttgaatt ccaattgtgc gcggcgctct
     1981 tcgcccggct tcagcggaaa aagctgcgcg gtgattttga gcacgccgtc gtgatgcgta
     2041 tacacggcga atgcgacgac ctcactgcga ggcacgtcca tcggcggcgg ctggcccccg
     2101 gctgctgctt tggccttcgc cttccccttc gccttgcctg ctgccgcggc gactcccttg
     2161 gtccaccact cgcccgtcgt tagcgactcc aaattgggaa actcggttcc gaacggctgg
     2221 ggagcgatct cttgagcgaa gagcgccgca ggcgagagag cgagcgctcc tgccccggcg
     2281 gcggtggctt gaatggcggt ccggcgattg atcttgttca tggggtacgg ctcggaatga
     2341 gaatttgcga gcgtcgatga tcgccatggt actgcgaaat tgccggagat tctctatgtt
     2401 gcaaataatc gcgcatggcg gcgatcggcg acgagccccg ccaccccgcc accccgcgca
     2461 ccacgaatcg aagaaggagc cgcgtgcgga tcaagtgaat tccggccact tctattacga
     2521 ccccctgttt ctcgaacacg agacgggccg gcatccggaa aatgccgggc gaatcagcgc
     2581 gctcggcagc cgcgtggatg acttggttgc gcaatatcgc tgcacgcggg gaagctggga
     2641 accgctttca gcgagcgacc ttaccgcggt tcacgatgcg gcgtacgtcg aaacagtcca
     2701 gagagtggcg gagcgcggcg gcgggatgct cgacccggat acggtcgtct cgccgcggtc
     2761 gtacgaggtg gcccgctcgg ccgcgggggc ggtcgccagc gcggtggata acgtgctggc
     2821 aggcgaagcc cggcgggcat tttgcctgct gcgtccaccg gggcatcacg ccttgcgcga
     2881 ccgcggcatg gggttctgcc tgttcaacaa catcgccatc ggtgcgcggc gggcgatcga
     2941 caagcatggc ttggcgcggg tgctcgtcgt cgattgggac gtgcatcacg gcaacggcac
     3001 gcaggagctg ttttgggagg atccgcaagt cggcttcttt tccattcacc gctatccgtt
     3061 ttatcccggc accggcgccg ccgacgaaac ggggggcggc gcgggcttgg ggacgaagct
     3121 caacgtgcca gtcgcgtatg gaacatctcg ccgcgaatac ctggacttgt tccgtggcgg
     3181 gctggaaagc ctggctgaga aaatgcggcc ggaattgatc ctgctgtctg ccggattcga
     3241 cgcccaccgg ctcgatccca ttggctcgct gggcctggaa agcgaagact tcgtgaccct
     3301 gacgcaatcg gtcgtgcaaa tcgccgattc ttattccggc ggcaagatcg tcagcgttct
     3361 cgaagggggc tacaacccag cggccgtggc ccagagcgtc cagttgcatc ttgagcagct
     3421 ctttttggac tgaacccgca accgacccag cgttgccgac cacgcaatcg aggcatatac
     3481 tacgggactt gatcgattga aaagggacaa gtctcgcttc gccggatttc cgtaaagcca
     3541 ttccggaaaa cgggttagag tggcaggcca gccctgctcg tggagccatt gaaatgacca
     3601 tcgacaaaag cctgcgggtc aaagcgggcg cgatccagaa ccgcaacgtg ctctcccgtg
     3661 ccgagcggat cgccaagctg aaagaagcgg aacgctggaa ggagggccag agcgtactcg
     3721 ggctgccgaa ggtccgcgtc caaaagctcg cgctcaagaa gaaaaagaag gccaag
//
LOCUS       DASUGB010000906         6335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1907308, whole genome shotgun sequence.
ACCESSION   DASUGB010000906 DASUGB010000000
VERSION     DASUGB010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907308"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFV87_17195"
     CDS             complement(<1..540)
                     /locus_tag="VFV87_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5105560.1"
                     /translation="MNVGLRLRDTAARDPAGIAIAMPRGRDADGKRIYQTLTFRELED
                     DSNLLAEGLAWMGVRPGMRLALMVPPSVDFISLVFAMFKAGVVSVLIDPGMGRRNMIR
                     CLADSRPDGFVGIPLAQAVRSLLRSKFPQAKYNVTVGRRWFWGGRTLRQLRARSRSAG
                     FQPAPTNADDPAAIIFTTGS"
     gene            741..1514
                     /locus_tag="VFV87_17200"
     CDS             741..1514
                     /locus_tag="VFV87_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105561.1"
                     /translation="MTTTQHCLSKIALGWTGAALIALAGCDITGEYEKRFQETLTTAG
                     HRAAFDAVLFAAETEITGEANASSGVKLRLPSLFDNSSKSLATEPRAQPPFVKLPGFG
                     YALERALPDDANQFAPAYIYFAAVPKADLKPDQAQAPIAVAVAAAFPGATWSDVQVST
                     PQGGSITHKVIRGDGPQDFDNAGTGGAVQKLDGRFALYFIEGPNHYALVGFRAPKAQA
                     DKWNFDAAVQAAMGTVTVASAPAAPADAGGAAAATPAGA"
     gene            1798..3003
                     /locus_tag="VFV87_17205"
     CDS             1798..3003
                     /locus_tag="VFV87_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5105562.1"
                     /translation="MSQPSNRPPREPGPSTLAVHAGEERQKAVNSITDPIVCASTYTF
                     ASTQAVIDYIEQKQNREEYGRYGNPGEKVVERKLAAMEGGEEALLFATGMAAIVGLLM
                     AKLNAGDEVVFFDECYHRSREFCAKHLSRFGVVTRQVPACDYDAMSAAISDKTKLLIS
                     ESPTNPHLSVVDLARFAELGKKHGVETLIDATLATPYNVQPLSYGVDYVLHSATKYLG
                     GHNDLLAGVIIGSREKLEPVRKLRGIMGAINSPHNIYLLERGLKTFELRMQRHNENGQ
                     RIAEFLAGHRRVERVYYPGLPSHPYHAIASQQMRGFGGLVTFLVKDADWKATAAIVDR
                     AQIARIAPSLGGVESLIEQPLVMSYYEVPPEDRRRFGIPDNMIRLSCGIENAEDLIED
                     LRQALGTGA"
     gene            3129..3257
                     /locus_tag="VFV87_17210"
     CDS             3129..3257
                     /locus_tag="VFV87_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105563.1"
                     /translation="MYAETPSGRRIWVIWRYDREDDEIPDVFGELGDAPIFVITAY"
     gene            3268..3519
                     /locus_tag="VFV87_17215"
     CDS             3268..3519
                     /locus_tag="VFV87_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105564.1"
                     /translation="MAEPSTDQRSPARRALDEEIAAEAPLSHPQLPLQAAPAPMEAHL
                     AQLRRPVAISGIVQNGAVYPVDPSIRLPEHSRVIIVAAE"
     gene            complement(3587..5074)
                     /locus_tag="VFV87_17220"
     CDS             complement(3587..5074)
                     /locus_tag="VFV87_17220"
                     /inference="COORDINATES: protein motif:HMM:NF025642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HEX5105565.1"
                     /translation="MSSTADTAQAIYVRDADFREHLKALGLASPDEYVPWCARRGFSM
                     RLDKHWRERARERYFDARERVHQRIQQSKAERRNPLLTWDRVLQGQQHSEALPPPWRL
                     IYERFSFLDDPAVKTALSGLLVQCHAQSRLITLQRAIPALRDQPGNTFAEGLIELAKT
                     CAAWIRPISAWVPRTRNKASQFASLAAHLLARYPVPPVMHCVWFRGPSLAACQQRAWY
                     LALAAGKSPRTLDFPLAFTRLMASHFQSSPKELLVEEALRRAQVLALGGNMRLARSIL
                     DSRLGTNFANNEFWTTVIRWFAKNPDLEQRHVAPLIDYIHQQKFEPQILPAGPAGGEH
                     VPPPPAPEFGMQGRTAGALLHQMHQWHARLRNESEASRSRLEWSACGVAPFESCEGRL
                     AAGDLRRWTIVELLSRQALFEEGRQLRHCVAIYAGSCAGGGTSIWSLGVERNACPRKR
                     VLTIELSVNRQVIRQVRGKANRLPTQKEIEIVQAWALQERLTYSP"
     gene            5319..5525
                     /locus_tag="VFV87_17225"
     CDS             5319..5525
                     /locus_tag="VFV87_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105566.1"
                     /translation="MKTRSRPTRDQDPATQPDVDDGETLAEKISRYCTDDFADKLTRH
                     FHRAKRRALAELDTATTKDENRSA"
     gene            5534..6175
                     /locus_tag="VFV87_17230"
     CDS             5534..6175
                     /locus_tag="VFV87_17230"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5105567.1"
                     /translation="MSVPELWIVAGPNGAGKTTLASLEPIASLLRSNQVLNPDDRCLS
                     LLQQQGLPGFSSAPLDVLQATFLKAADEVLAELQAAIAANQAVLVETVLSTPKYRAIV
                     DEVRSRGGFFGLIYVALKSPELAFERVQSRARLGGHDVPGDRVATRWRRSLEHLPWFA
                     ARSQRLLVYDNSNSVPTAAPLLVADGIGGRVTIHHPTAIPELTAALRQVSPAQ"
     gene            6184..>6335
                     /locus_tag="VFV87_17235"
     CDS             6184..>6335
                     /locus_tag="VFV87_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5105568.1"
                     /translation="MHFRTRAIHVGQERDPATGAVVPPIHVASTFVQPGAGVWSEFDY
                     SRSGNPT"
BASE COUNT         1207 a         1956 c         2010 g         1162 t
ORIGIN      
        1 gctgccggtt gtgaagatga ttgctgccgg atcgtccgcg ttagtcggcg cgggctggaa
       61 gcccgcgcta cgcgaacggg cgcggagctg gcgaagcgtt ctgccccccc agaaccagcg
      121 gcggccgacg gtgacgttgt acttcgcctg cgggaacttg ctgcggagga gcgaccgcac
      181 ggcctgcgcc agcggaatgc cgacaaaacc gtccggccgg ctgtcggcca ggcaacggat
      241 catgttccgc cggcccatgc cggggtcgat cagcacactg acgacgcccg ccttgaacat
      301 cgcgaacacc agcgagatga agtcgacgct cggcgggacc atcagcgcca ggcgcatccc
      361 gggccgcaca cccatccagg ccagcccctc ggccaggagg ttgctgtcgt cttcgagctc
      421 gcggaacgtc agcgtttgat agatccgctt cccgtccgca tcgcgccccc gcggcatggc
      481 gatggcgatg cccgccggat cgcgggccgc cgtgtcacgc agccggagac cgacgttcac
      541 aaccgtacgt ctggaaacaa gcgccaggtc agtcattccg atcaagcggt atcgggtggc
      601 tcgcggaatg cagttcattc tacctgcccg aatggctgtc gttcgcggca aacgctcgtg
      661 gattgcgacg attcattgtg caaggcaaga tagagtgttc cctggtgctc ccttcacgca
      721 actggctctg aattcttgcc gtgactacca cacaacattg tctgtcgaaa atcgcccttg
      781 gttggacggg ggcagcctta atcgctctgg ccggttgcga catcaccgga gaatacgaga
      841 agcggtttca ggaaacgctg acgaccgccg gccaccgagc ggcttttgac gccgtgttgt
      901 tcgccgccga aacggaaatc accggagaag cgaacgcgtc gtccggcgtc aagctgcggc
      961 tgccgtcgct gtttgacaat agcagcaagt cgctggcaac cgagccccgc gcccagccgc
     1021 cgtttgtcaa gctgcccggc ttcggctacg cgctggagcg agccttgcca gacgacgcca
     1081 accaattcgc ccccgcgtac atttacttcg cggccgtgcc caaggcggac ctgaagcccg
     1141 accaggcgca ggcgccgatt gcagttgccg tggcggctgc atttccaggt gctacgtgga
     1201 gcgacgtgca ggtcagcacg ccgcagggcg gaagcatcac gcacaaggtg atccgcggcg
     1261 atggcccgca ggacttcgac aacgctggga ccggcggcgc cgtgcagaag ctcgacggcc
     1321 ggttcgccct gtatttcatc gaggggccga atcactatgc cctcgtcggc ttccgcgcgc
     1381 ccaaggctca ggccgataaa tggaacttcg atgccgcggt ccaggccgcg atgggaacgg
     1441 tgacggtcgc atccgccccg gcggccccgg ccgacgccgg tggggctgcc gccgcaactc
     1501 ccgcaggcgc ttgatgccga gcaagtcaat cggagcgctt gttccgggtc cgggaacaag
     1561 gtatagtttc ctcaacaagg gatttgcggc cgcttgcagc cttacctgct aaagagccat
     1621 tcacctgtcc cgatggagtt gaattcgcgc accggcacat gaaagtgact gcgcggatgt
     1681 gaaacgggac ttctgattgg aggctcgccc aagacgggca gagccgctct cagcccattg
     1741 catgctcgcg agctggacgc ggttccctgc gcttttcgcc ttggagctcg gttagccatg
     1801 tcgcaaccat cgaaccgccc cccgcgcgag cccggtccat ccacactcgc ggttcacgcc
     1861 ggagaggagc ggcagaaagc cgtaaactcg atcaccgatc cgatcgtctg cgcttcgacc
     1921 tatacgttcg ccagcacgca ggcggtgatc gactacatcg agcagaagca gaaccgtgag
     1981 gagtatggcc gctacggcaa tccgggcgag aaggtcgtcg agcggaagct ggcggcgatg
     2041 gaaggaggcg aggaggctct cctctttgcg accggcatgg cggcgattgt cggcctgctg
     2101 atggccaagc tgaacgccgg cgacgaggtg gtattcttcg acgaatgtta ccaccgcagc
     2161 cgcgagttct gcgccaaaca cctttcgcgg ttcggcgtgg tcacgcggca agtgccggcc
     2221 tgcgattatg acgcgatgtc cgcggcgatc agcgataaga cgaagctgct gatcagcgaa
     2281 tcgccgacca atccgcattt gagcgttgtc gatctggctc gcttcgcaga attgggaaag
     2341 aagcacggcg tggaaacgct gatcgacgcg acgctggcca cgccgtacaa cgtccaaccg
     2401 ctgagctatg gcgtcgatta cgttctgcat tcggcgacca agtatctggg cggccataac
     2461 gatctcttgg ccggcgtgat catcggcagc cgcgagaagc tggagccggt tcgcaagctg
     2521 cgcgggatca tgggggcgat caattcgccg cacaacattt acctgctgga gcgggggctc
     2581 aagacgttcg agctgcgtat gcagcggcac aacgaaaacg gccagcggat tgccgagttc
     2641 ctggcgggcc atcgccgagt cgagcgggtc tattaccccg gcctgccgtc gcacccctat
     2701 cacgccatcg ccagccagca gatgcgcgga ttcggcggcc tggttacgtt cctggtgaag
     2761 gacgccgatt ggaaggcgac cgcggcgatc gtcgatcggg cccagattgc ccgcatcgcg
     2821 cccagcctcg gcggcgtcga atcgctcatc gagcagccgc tggtgatgag ctattacgag
     2881 gtcccgcccg aggaccgccg ccgcttcggc atcccggaca acatgatccg cctctcctgc
     2941 ggcatcgaga atgcggagga tttgatcgaa gacctgcggc aggcgctggg aaccggcgca
     3001 tgagaagctt caagtggata gaatggaacc tgcagaagat cgacgcgcac gctctgtcgg
     3061 ctgaagaagt cgaagctgcg tttgaccgtg tgttcacgct gcaggagtga aatgacggct
     3121 cgttcgagat gtatgccgag acgccgtcgg gtcgccgaat ctgggtgatc tggcgctacg
     3181 accgcgagga cgacgaaatc ccggatgtct ttggcgaact gggcgacgcg ccgatcttcg
     3241 tcattaccgc ttactgagag aatcgccatg gccgaacctt cgaccgatca acgcagcccc
     3301 gcccgccggg cactcgacga agagatcgcc gctgaggcgc ctctgtcgca tccgcagctt
     3361 ccgctgcagg cggcgcctgc cccaatggag gcgcatctgg cacaactgcg ccgccccgtg
     3421 gcgatttccg gcatcgtgca aaacggcgcc gtctatcccg ttgatccaag tatccgactg
     3481 ccggaacact cgcgagtcat tatcgttgcg gcagaatgaa tttacctttc ctatactcac
     3541 gaccgacacc gaaccaggtg ccggaggcgt gaggtctcgc cgactgtcac gggctgtacg
     3601 tcagtcgctc ctggagcgcc cacgcttgaa cgatttcgat ctctttctga gtgggtagcc
     3661 gattggcctt gccgcgcacc tgccgaatca cctggcggtt gacggagagt tcgatcgtca
     3721 gcacccgttt gcgggggcat gcgttgcgtt ccaccccgag cgaccagatg gaggtgccgc
     3781 cgccggcgca cgagccggcg tagatcgcaa cacagtggcg caactgtcgc ccctcctcga
     3841 atagcgcctg ccggctcagg agctccacga tcgtccagcg ccgcagatcg ccagccgcga
     3901 gcctgccctc gcacgattcg aacggcgcga ccccacaagc gctccattcc agcctgctgc
     3961 gcgatgcttc gctttcgttt cgcagtcgtg cgtgccactg gtgcatctga tgcagtagcg
     4021 cgccggcagt tcggccttgc atgccgaatt ccggcgcggg aggcggcggc acgtgctcgc
     4081 cgcccgcagg accagcagga agaatctgcg gctcaaactt ctgctggtgg atgtagtcga
     4141 tgagcggagc gacatgccgc tgctccaagt ccggattctt ggcaaaccag cggatcacgg
     4201 ttgtccagaa ttcgttgttg gcgaagttcg tccccagccg cgagtccaga atcgagcgcg
     4261 ccagccgcat gtttccaccg agtgccagga cttgggctcg gcgcaaggcc tcttccacca
     4321 gcagctcctt cggcgagctc tgaaagtggc tggccatcag ccgcgtgaaa gcgaggggaa
     4381 agtcgagcgt gcgcgggctc tttcccgctg ccagcgccag ataccaggct cgctgctggc
     4441 acgccgcgag cgaaggaccg cgaaaccaca cgcagtgcat gacgggcggt acgggatacc
     4501 tggccaggag atgggccgcg agtgaggcaa actggctggc tttgttgcga gttctcggca
     4561 cccacgcgct gatgggtcga atccacgcgg cgcacgtctt ggccagctcg atcaggcctt
     4621 cggcaaatgt gttccctggc tggtcgcgca gagctggaat cgcgcgttgc aacgtgatga
     4681 gtcggctctg ggcgtggcat tggactagca ggccgctgag cgcggttttc actgcgggat
     4741 cgtccaagaa gctgaatcgc tcatagatca gccgccacgg cgggggcaat gcttccgaat
     4801 gctgctggcc ctgaagcacg cggtcccacg tcagcagcgg atttcgccgt tcggccttgc
     4861 tctgttggat tcgttggtgg acccgctcgc gggcgtcgaa gtagcgttct cgggctctct
     4921 cgcgccagtg cttgtcgagg cgcatgctga agccgcgcct ggcacaccag gggacgtact
     4981 cgtctggcga ggctaaccca agggccttga ggtgttctcg aaaatcagcg tcgcgaacgt
     5041 agatggcttg tgcggtgtct gcggtcgagg acatgcggat ggagaatggt cgtgtacaag
     5101 gcgctggttt gttgggaatg gagtgcgaaa cggtaactgg cttgggaacg caacacgctc
     5161 ttgtctccta cgggaaagtg aagtcaaatg ccgactcgaa ggacagagac tgagacgcaa
     5221 ggttcacggc tgaggaactg ccagaattcc tcgtctaagc ccgccagcgc gtgcctggga
     5281 gtaagcggtg gagtagaatt caagaacgag gacaagtgat gaagacgcgc tccaggccaa
     5341 caagagatca agatccggca actcagcccg acgtggacga cggcgaaact ctcgccgaaa
     5401 agatcagccg ctattgcacc gatgactttg ccgacaaatt gactcgccac tttcatcgcg
     5461 ctaaacgcag ggcattggct gagcttgaca ctgcgaccac aaaggacgaa aaccgctcgg
     5521 cgtagtgcta ttcatgtcgg tccctgaact ttggatcgtc gccgggccga atggggccgg
     5581 caaaacaacg ctggcaagtc tcgagccgat tgcgagtttg cttcgctcga atcaggtgct
     5641 gaaccccgac gaccgctgcc tgagtctgct gcaacagcaa ggtttgccag ggttctctag
     5701 cgcaccactg gacgtacttc aggccacttt cttgaaagca gccgatgaag tgctggcgga
     5761 attgcaggcg gcgattgccg cgaaccaagc cgtgctggtc gaaaccgtcc tcagcacgcc
     5821 caagtatcga gccattgtgg acgaagtgcg atcgcgaggc ggtttcttcg gactgatcta
     5881 cgtcgctctc aagtccccag agctggcttt tgagcgcgtc caatcgcgag cacgtctggg
     5941 aggacatgat gtgcctggcg atcgcgtcgc tacacgctgg cgccggtcgc tggagcattt
     6001 gccctggttc gctgcccgtt cacagcgtct cttggtttat gacaactcaa attctgttcc
     6061 gactgcagca ccactactcg tggcggatgg gattggaggg cgagtgacga ttcatcatcc
     6121 gactgccatt cccgagctga cggcagcttt gcggcaggta agcccagccc aatgacttct
     6181 caaatgcact ttcgcacacg cgcaattcac gtcggtcagg aacgcgatcc cgcgacgggg
     6241 gcggtggtgc cgccgattca tgtggcgagc acgttcgtgc agccgggggc gggagtgtgg
     6301 tccgagttcg attattcgcg gagcggcaat ccgac
//
LOCUS       DASUGB010000907         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_241618, whole genome shotgun sequence.
ACCESSION   DASUGB010000907 DASUGB010000000
VERSION     DASUGB010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241618"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1837)
                     /locus_tag="VFV87_17240"
     CDS             complement(314..1837)
                     /locus_tag="VFV87_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HEX5105569.1"
                     /translation="MNHPLPLPMNRSRLQPACVRLLLAIFVVHLLAASVSAQSTLPDT
                     TAPRDELLAQRPATLKRPPPELSAPAAKTASRSASAGRSASDRTAQAEVLPPATRAVP
                     AAPSGPTIILPGMPEQGYVMDDPDGYGALDGYPAYDYDYGLAGPAGGRFWVRGEYLLW
                     WVEGMRAPPLVTTSTAGTPQADAGILGLPSTSILFADRRLLEQYRHGVRLTAGGWFGD
                     AHAFGAEASAFWLMTDHDDFSANSGDFPILARPFFNVEPGFEGPDAEIIAYPGLFVGQ
                     IDVASDTSLFGGDVLLRACLCQDCYCRVDGLLGARYARLEDTLQISDNRTVIGAGAGV
                     AIGTTFRELDLFETRNEFIGGELGFSSQMKFRRFWLEGVLKLALGVNRSRVQISGQST
                     ATIPVPGGAPIVVNTPAGLLAQQTNIGVYKDDSFSVIPEVGLTVGFEITPRLRATAGY
                     TFFYWTDVLRAGDQIDTNLNLSQLAPGGLVGIPRPQFTGVTDDLWVQGLSLGLDFRF"
     gene            complement(1834..2940)
                     /locus_tag="VFV87_17245"
     CDS             complement(1834..2940)
                     /locus_tag="VFV87_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105570.1"
                     /translation="MSRRIRRSRNSQNSRPLQVERLEERSLLAVAAFTINLREDLGGT
                     PGEILAADEVTAGECFFVEITAQEFDPLAHGLNGVALNLAWDPAALELEGSFDPAEII
                     TDNLPMFRHGTLNAAAGTIRDLSGAAAEIWGAGRPIGDGTAETFAMLHFRATGDTSAA
                     SIRMSEGVSRIVTTPAATFTSAQLSFEQQTIAVAPANPAAVASTAEPPAPEPEFVPPP
                     SQPVVEPQPEPLPPPAVELPTQTPIIATPPASEFTLEVPAAAPIGPFVDSTLDPPSSD
                     EPAGEPIAFAPPSPAPASFAPAANACPAESPPPAAARLAAATDVAVALMGPLTPEQFH
                     GPLLRLEQVIALIAADIAERRRHTALNSPSPLGP"
BASE COUNT          491 a          959 c         1077 g          482 t
ORIGIN      
        1 agccgccccg aacttacaac gacacgactc cctataacct cctgccccac catcacttac
       61 agcgccaccc ctcgccctaa cttttctcca ccccgccggc caagaaggac aagtctggtc
      121 cattccgccc accgctcatc cgggtggtac tggaagaatc tcgtcgtacc gggcttcgtt
      181 ttgaaaaccc ctgaagacat tggggtgccc gggggttggc gatagctcta ccccagcaaa
      241 tccgggttat tgcttcgcaa aaaccggcca ccctgggcca ggagcaagag acgcctactt
      301 gcgagtccgc tactcagaaa cggaagtcga ggccgaggct gaggccctgg acccagaggt
      361 cgtcggtgac gccggtgaac tgcggccggg ggatgccgac caggccgccg ggagcgagtt
      421 ggctgagatt caggttggtg tcgatctggt cgccggcccg cagcacgtcg gtccagtaga
      481 agaaggtgta gcccgcagtg gcccgcagcc gcggcgtgat ctcgaagccg accgtcaggc
      541 cgacttcggg aatgacggag aacgagtcgt ccttatagac gccgatgttg gtttgctggg
      601 cgaggagccc ggccggggtg ttgacgacga tcggcgcgcc gccgggaacg ggaatggtgg
      661 cggtcgattg gccgctgatc tggacccgcg agcggttgac gcccagggcc agcttcagca
      721 ccccttccag ccagaagcgg cggaacttca tttgcgagct gaagccgagt tcgccgccga
      781 tgaattcgtt tctggtttcg aacaggtcga gctcgcggaa ggtcgtgccg atggcgaccc
      841 ccgcgccggc gccgatcacc gtgcggttgt cgctgatttg cagcgtgtct tccaggcggg
      901 catagcgcgc cccgaggagg ccatcgacgc ggcagtagca atcctggcag aggcaggccc
      961 gcagcagcac gtcgccgccg aacaggctcg tatcggaggc cacgtcgatc tggccgacga
     1021 acaagccggg gtaggcgatg atttccgcgt cggggccttc gaagcccggt tcgacgttga
     1081 agaacgggcg ggccaggatc ggaaaatcgc cgctgttggc ggagaagtcg tcgtgatcgg
     1141 tcatcagcca gaacgcgctg gcttcggcgc cgaacgcgtg ggcatcgccg aaccagcccc
     1201 ccgcggtcaa ccgcacgccg tgccgatatt gctccagcag ccggcggtcg gcaaagagga
     1261 tgctggtcga ggggaggccg aggattccgg cgtcggcctg cggcgtgccg gccgtgctgg
     1321 tcgtgaccag cggcggggcc cgcatgcctt cgacccacca caacaggtac tcgccgcgga
     1381 cccagaatcg cccgccggcc gggcccgcca gcccgtagtc gtagtcgtac gccggatagc
     1441 cgtcgagcgc gccgtagccg tcgggatcgt ccatcacgta gccctgctcc ggcatgcccg
     1501 gcaggatgat cgtcggtccg ctcggcgcgg ccggcacggc ccgcgttgcc ggaggcagca
     1561 cttcggcctg ggccgttcgg tcggaagcag atcggccagc ggatgcggag cggctggcgg
     1621 ttttcgcggc gggagcggaa agctccggcg gcggacgctt gagcgtggcc ggccgctggg
     1681 cgagcagctc gtcgcgcggg gccgtcgtgt cagggagcgt gctctgagcg gagacgctgg
     1741 ccgccagaag atgcacgacg aaaatcgcca acagcagccg gacgcaggcc ggctgcaggc
     1801 gcgagcggtt catcggcagt ggcagcggat ggttcacggt ccgagggggc tgggtgaatt
     1861 gagggcggtg tgcctgcggc gctcggcgat gtccgcagcg atcagggcga tcacctgctc
     1921 caggcggagc aatggcccgt ggaattgttc gggcgtcaac gggcccatca gtgcgacggc
     1981 aacatcggtc gcggcggcga gcctggccgc ggccggcggc ggactctccg ccgggcaggc
     2041 gttggctgcg ggcgcaaagc tcgccggggc cggcgatggc ggcgcgaagg cgatcggctc
     2101 gccagccggc tcatcgctgc ttggcggatc aagtgttgaa tcaacgaatg gtccgatcgg
     2161 cgcggccgca ggcacttcca gcgtgaattc cgaagcggga ggcgtggcga tgatcggcgt
     2221 ttgggtgggc aattcgacgg ccggcggcgg caacggctcg ggctgcggct caacgaccgg
     2281 ctggcttggc ggcgggacga actccggctc tggcgcgggg ggctctgcag tcgatgcgac
     2341 tgctgcgggg ttcgcaggcg ctacggcgat cgtctgctgc tcaaagctca gctgggcgct
     2401 cgtgaaggtc gcggccggcg tggtgacgat tcgcgagacg ccttcgctca tccgaatcga
     2461 agcggccgac gtatcgcccg tcgcgcggaa gtgcagcatc gcaaaggttt ctgccgtgcc
     2521 gtcgccgatc gggcggcctg cgccccaaat ctcggcggca gcgccgctca ggtcgcgaat
     2581 cgttcccgcg gcggcgttga gtgtgccgtg ccggaacatc ggcaagttgt cggtgatgat
     2641 ctccgccgga tcgaacgacc cttcgagctc cagcgccgcg ggatcccagg cgagattgag
     2701 cgcgacgccg ttgagtccgt gcgccagcgg atcgaattct tgcgcggtga tctcgacgaa
     2761 gaaacattcg ccggcggtga cttcgtcggc ggccaggatt tcccccggtg taccgcccag
     2821 atcctcgcgc aggttgatgg tgaacgccgc gaccgccagg agcgaacgct cctccagccg
     2881 ctcgacctgc aaggggcgcg agttctgcga attccgggac cgccgaatcc ttcggctcat
     2941 cggggggcat cctgccgcga gtgagtcctt ccctttgccg cgtgcgagtg cggctctggc
     3001 tcaagaaaa
//
LOCUS       DASUGB010000908         4948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1907527, whole genome shotgun sequence.
ACCESSION   DASUGB010000908 DASUGB010000000
VERSION     DASUGB010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907527"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VFV87_17250"
     CDS             complement(<1..1225)
                     /locus_tag="VFV87_17250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5105571.1"
                     /translation="MLALALPVLAEESLNLLVGYTDWFLAGRFLPGDESLAAMNFVAY
                     FLWILPSLFAGVGIGALAVIARLVGAGQSREAAHVARQALYLGCGVALLGMAAVALLG
                     GSFVEALQLRGEAAVLALRYVRILTPAIPFIMLEQVGSACLRGAGDTVTGMFARIVLN
                     VVNVAVSTSLVLGLGPLPKLGFDGLAIGTACGHAVGGLIVLYRLTRSSAGLSLLQPES
                     DSSRRLRWDGPTIRRILRIGLPGGIDVLAVLTCHLIYVSIINRLGTLAQAAHGLGVQI
                     EAMSYLPGSAFQVAAATLAGQSLGAADQRRATRGVLLCVASALAIMTAAGLVLFFFGE
                     PVARVFTGESTDTTRLTGRLLRIVALSCPSLAILMVLSGALRGSGDTRASLAITFVGL
                     AGIRIPAACLLAWNEV"
     gene            1402..2640
                     /locus_tag="VFV87_17255"
     CDS             1402..2640
                     /locus_tag="VFV87_17255"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5105572.1"
                     /translation="MLKKTSHRAGRTRTQPTGYSAFIPEKLPPAPPLIVDLTMQQLLQ
                     NASLELGRLDGLASILPNPDGFVTAFVRKEALLSSQIEGTQASLDEVLAFEAAERTRT
                     SHERTGAPEVYRYVKAMKYGLGRLNELPLSLRLIKEIHGVLLDTGRGSDKSPGEFRKS
                     QNWIGSPGCTLKEATFVPPPPLEMNAALSELEKYLHKDNGLPLLVQIALVHAQFETIH
                     PFLDGNGRVGRLLITLLLCHAKVLRRPLLYLSYFFKQRRSEYYERLDAVRFAGAWEGW
                     VMFFLQGIVEVARQAVQAATQIIELRERHFALIHRKLPRASTKAIQLLDYCFQHPYLT
                     ATDVTTLLRVSGPTARSQIAKLASLGVLEEITRRNWGRVFAYREYIDILREGTELPSE
                     NRPTQKAATARKQVTNPASK"
     gene            2776..3876
                     /locus_tag="VFV87_17260"
     CDS             2776..3876
                     /locus_tag="VFV87_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5105573.1"
                     /translation="MAHEVTLITGDGTGPELAAAAKMCIDATGVRINWDEQEAGVDVM
                     ARTGTPLPDSVLASVRRTKCALKAPITTPVGKGFRSINVHLRQELGLFACIRPCKYYP
                     GVRSYFSEVGVNIVIVRENTEDLYAGVEFEAGKPATAELIDFINKIATDKKIKTNAEE
                     TGVSIKPISKSGTERIVRCAFDYARKNGRKKVTAVHKANIMKFSDGLYLHTATAVAKD
                     YPDIEFEERIVDNMCMQLVQKPELYDVLVLPNLYGDIVSDLGAGLVGGLGVAPGANIG
                     PGGAVFEATHGSAPKYKGLNKVNPTAVILSGMLMLRHLGESEAGDRLERAVADVIAEG
                     KNVTYDLKPDRNDPTAVGTREMAQAICAKLKK"
     gene            3898..4368
                     /locus_tag="VFV87_17265"
     CDS             3898..4368
                     /locus_tag="VFV87_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105574.1"
                     /translation="MSGNFGDFNPYASPPFAPEPPTFRRQVEGKVKPPAIVLIVAAVL
                     GLGFSLFNVAFSFTEPQVDPNAPEFLREMQQGAVGPVAGAIQGAFAAINLLVLVGAVQ
                     MMRLKMWGLAVAASVVAMVNFGSCCCVLGLPVGIWSLVVLLSPEVKAAFAAPSR"
     gene            complement(4509..>4948)
                     /locus_tag="VFV87_17270"
     CDS             complement(4509..>4948)
                     /locus_tag="VFV87_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5105575.1"
                     /translation="WAFYQSMLDFFVKSPYLLTRRGLAHMKRQKWGRIINITSEVFQR
                     SVAPFSAYVAAKGGQIGWSRSMSQELAPFGITVNMVAPGWIPVERHEKDPQSEKDAYQ
                     ALIPCGRWGTPQDVADAVLYFASDEASFVTGQTLCINGGMTPW"
BASE COUNT         1027 a         1468 c         1606 g          847 t
ORIGIN      
        1 gaacctcgtt ccacgcgagc aggcaggcgg caggaatgcg gataccggcg aggccgacga
       61 acgtgatcgc gaggctggcc cgcgtatcgc ccgagccgcg gagcgcgccg ctgagcacca
      121 tgagaatggc gagcgacgga caagagaggg cgacgatcct gagcagccgg ccggtgaggc
      181 gcgtcgtgtc ggtcgattcg ccggtgaaca cgcgggccac gggctcgcca aagaaaaaga
      241 gcaccagacc cgcggcagtc atgatcgcca gggccgaggc gacgcacagg agcacgccgc
      301 gcgtggcccg ccgctggtcg gctgcgccga gcgattggcc ggcaagggtc gcggcggcga
      361 cttgaaacgc cgagccgggg aggtacgaca tcgcttcgat ctgcacgccc aggccgtggg
      421 ccgcctgggc gagcgtccca aggcggttga tgatcgagac gtagatcagg tggcaagtga
      481 gcacggcgag gacatcgata ccgccgggca ggccgatacg gaggatgcgg cggatggttg
      541 ggccgtccca gcgcaggcgg cgcgagctgt cgctttcagg ctgcaggagg ctcaatcccg
      601 cgctggagcg cgtcagtcgg taaaggacga tcagtccccc aaccgcgtgt ccgcaagcgg
      661 tgccgatcgc cagcccatcg aacccgagct tgggcaacgg gccgaggccc aggacgagcg
      721 atgtgctgac ggcgacattc acgacgttga gcacgatgcg ggcaaacatg cccgtgacgg
      781 tatcgcccgc gccgcggagg caagccgagc cgacctgctc cagcatgata aaggggatgg
      841 cgggcgtaag gatgcgcacg tagcggaggg cgagaacggc cgcttcgccg cggagttgca
      901 gcgcttcgac aaacgagccg ccaagcagcg cgaccgccgc catgccgagc agcgcgacgc
      961 cgcagccgag atagagcgct tgccgggcga cgtgggcggc ctcgcgcgat tgtccggcgc
     1021 cgacgaggcg ggcgatgacc gccagcgccc cgatgccgac gccggcgaag aggctcggca
     1081 gaatccagag gaagtaggcg acgaagttca tcgccgcgag gctttcgtcg ccgggaagaa
     1141 agcggcccgc cagaaaccag tccgtatagc ccaccagcag gttgagcgac tcctccgcca
     1201 gaaccggcag agcgagggcc agcatggcgc gggtggtttc tttcaagtcg gcctcggcag
     1261 atcgacggaa gtcggccagt ataggcgact ggttggcacg gctacgttga cttggaaata
     1321 aaacaggcat attctttcca agtgtctgaa ggatggtaag ttatcttgcg ttctgcgcac
     1381 ccccttaaag agtgtgtaca agtgctcaag aaaacttcgc atcgcgccgg tcgaacgcgc
     1441 acgcagccaa ccggctattc cgccttcatt ccagaaaaac tgcctccggc gcctccgctg
     1501 atcgtggatc tgacgatgca gcaattgctg cagaacgcct ctttggaact gggccggctc
     1561 gatggcctgg cgtcaattct gccaaatccc gacgggtttg tgaccgcctt cgtgcggaaa
     1621 gaagccctgc tgagctcgca aatcgaaggg acgcaagcat cgctcgacga agtgctggcc
     1681 tttgaggcag cggaaaggac aagaacgagc cacgagagaa ccggcgcacc cgaagtttac
     1741 cggtacgtca aggcgatgaa gtacggtctg ggcagattga acgaacttcc gctgtctttg
     1801 cgcttaatca aagaaatcca cggcgttctg ctcgacacgg ggcgggggag cgacaaatcg
     1861 cccggagaat tccgcaagtc acagaactgg attggatcgc cgggatgcac actcaaagag
     1921 gctaccttcg ttccaccgcc gccgttggag atgaacgcgg ctctctctga actcgagaag
     1981 tacctgcaca aggacaacgg acttcccttg ctcgttcaga tcgcgctcgt ccacgctcaa
     2041 tttgaaacga ttcacccgtt cttagacggc aatggacgcg tcggccggct gctgattacc
     2101 ctcctgcttt gccatgcgaa agttcttcgc cggccgctcc tatatctcag ctacttcttt
     2161 aagcaaaggc ggtcggagta ctatgaacgc cttgatgccg ttcgttttgc aggggcctgg
     2221 gaggggtggg tcatgttctt tctgcaaggc atcgtcgaag tggcgaggca ggcggtgcaa
     2281 gcggcgacgc agattatcga gcttcgcgag cgccacttcg ccctgatcca ccgtaaactg
     2341 ccgcgcgctt ccaccaaggc gatccagctt ctggactact gttttcagca tccctatctc
     2401 acagcaaccg acgtcacaac tttgttgcga gtcagcggcc ccacagccag aagccagatc
     2461 gccaaactgg cgtcgctcgg cgtcctggaa gaaatcacca ggagaaactg gggaagagtt
     2521 tttgcgtacc gggaatacat cgatatcctg cgagaaggca ctgagttgcc ctcggaaaac
     2581 aggccgaccc aaaaggccgc gactgcgcgc aagcaagtca ccaaccccgc atcaaaatag
     2641 gcggtgcaga ttgcacctcc tggttcccaa gcagagcttg ggaccgagga actccagata
     2701 caggtggctt gtcgcggttg gcggcgcgtg ttacttttgc cagccagacc aattctctgc
     2761 gaggagcgat ttgcgatggc ccatgaagtg acgttaatca ccggcgacgg caccggcccc
     2821 gaattggccg cggccgccaa gatgtgcatc gatgcgactg gcgtgaggat caattgggac
     2881 gagcaggagg cgggcgtcga tgtgatggcc cgcactggga cgccgctgcc ggatagcgtg
     2941 ctggccagcg tccgtcgaac caagtgtgcg ctgaaagccc cgattaccac gccggtcggc
     3001 aaaggctttc gctcgatcaa cgtgcacctg cgacaggagc tgggtctgtt cgcctgcatc
     3061 cgcccgtgca aatactatcc cggcgtgcgg agctacttca gtgaagtcgg cgtcaatatt
     3121 gtcatcgtcc gagagaacac cgaagacttg tatgccgggg ttgagttcga agcgggcaag
     3181 ccggcgaccg ctgagttgat tgatttcatc aacaagatcg cgaccgacaa gaagatcaag
     3241 acgaacgccg aggagacggg agtaagcatc aagccgatca gcaagagcgg caccgagcgg
     3301 atcgtccgct gcgcattcga ttatgcccgc aagaacggcc gcaagaaggt caccgccgtc
     3361 cacaaggcga acatcatgaa gttctccgac ggcctctatc tgcacacggc cacggcggtc
     3421 gccaaggatt atcccgacat cgaattcgag gagcggatcg tcgataacat gtgcatgcag
     3481 ctcgtgcaaa agccggagct gtatgatgtg ctggtgctgc cgaatttata tggcgacatc
     3541 gtgagcgacc ttggcgcagg gctggtcgga ggactgggcg tcgctccggg agcaaacatc
     3601 ggccccggcg gcgcggtctt cgaagcgacg cacggcagcg cgccgaaata taagggtctg
     3661 aacaaggtca atccaaccgc ggtgatcctg agcggcatgc tgatgctgcg gcacctgggt
     3721 gaatcggagg cgggagatcg cctggagcgg gctgtggccg acgtgatcgc cgaaggcaag
     3781 aacgtgacct acgacctgaa gcccgatcgc aacgacccga cggcggtggg cacgcgcgag
     3841 atggcccagg caatttgtgc gaaactgaag aagtgacagc ggggcgcggc cagcaccatg
     3901 agcggcaact tcggcgattt caatccctat gccagcccgc cgttcgctcc cgaaccgccc
     3961 actttccggc ggcaagtcga aggcaaggtc aagcccccgg cgatcgtact gatcgtggct
     4021 gccgtgctgg gattggggtt ctcgctgttt aatgtcgcgt tctcgttcac ggagccacaa
     4081 gtcgatccca acgcgcccga gttcctccgc gagatgcagc aaggcgccgt cggccccgtt
     4141 gccggagcaa ttcaaggtgc attcgccgcg atcaacctac tggtcctggt tggcgctgtg
     4201 cagatgatgc ggctgaagat gtgggggctg gcggtggcgg cgtcggtcgt cgcgatggtc
     4261 aatttcggca gctgctgctg cgtactgggg ttgcccgtag gcatctggag cctggtcgtg
     4321 ctgctgtcgc cggaagtcaa ggcagcgttt gctgcaccaa gtcgttaggg cgcagcaaag
     4381 accttgcgat gagatcggcg attgcctatc tggaggcagc tgcgattcgg ggaatccggc
     4441 tgcgggcgat catgtaccgc tcccaaccga cccgtggaga gaatttcgcc cgccgtttgg
     4501 cctgccgcct accacggcgt catgccgccg ttgatgcaga gggtttggcc ggtgacgaac
     4561 gaggcctcgt cgctggcgaa atagagaacg gcatcggcca cgtcctgcgg cgtgccccag
     4621 cggccgcagg ggatcagggc ttgataggcg tccttttcgg actgcgggtc cttctcgtgc
     4681 cgctcgacgg gaatccaacc cggggcgacc atgttcaccg tgatgccgaa gggtgctagt
     4741 tcctgagaca tactccgcga ccagccgatc tggcctcctt tcgccgcaac ataggcgctg
     4801 aacggagcaa ccgaccgctg aaagacctcg ctcgtgatgt tgatgatgcg gccccacttc
     4861 tgccgcttca tgtgggccag cccgcggcgc gtgagcaagt aggggctctt gacgaagaaa
     4921 tcgagcatcg actggtaaaa agcccagt
//
LOCUS       DASUGB010000909         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1351799, whole genome shotgun sequence.
ACCESSION   DASUGB010000909 DASUGB010000000
VERSION     DASUGB010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351799"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..>2071
                     /locus_tag="VFV87_17275"
     CDS             131..>2071
                     /locus_tag="VFV87_17275"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105576.1"
                     /translation="MATTVQDFPGTERFVIQQRLGAGSMGVVYQAYDRQQGDRVALKT
                     IKHLDPAAIYYIKQEFRALADVSHPNLVSLYELFSEGEQCFFTMQLLEGVDFLSYVCG
                     YGPATDAANGSEPTSAPVGAAETVAFVPGSKVDDRIQLTGGPSAPRPGSPVSLDRLRG
                     ALQQLVEGVCALHRAGKLHRDIKPSNVFVTRDHRVVLLDFGLATEATQQADETSAPHG
                     VIGTAIYMSPEQAAGLPATSASDWYCVGVVLYQALTGRLPIEGSKGRVLWYKQFSDPL
                     PPRELAPEIPEDLNALCVDLLCRDPAGRPTDEQVRERVGSAPGELAASIEPARKRASR
                     LVGRQQHLDALADAYASLQQGRTTVVYVHGRSGMGKSSLFERFLNDLRREDAVILSGR
                     CYEQESVPYKALDSLIDALSHYLVRLPDAQVEAVLPRDVASLARVFPVLRRVEAVAYA
                     PRRMAETPDQQELRRRALAALRELLLRLGDRRRLVLAIDDLQWGDADSAHLITELLRP
                     PDPPLLLLLGCYRSEYAETSHCLQVLLRPQEQASSSVERRELAVEPLSADEAGELALA
                     LLGSDSPAAQAAAQSIARESRGNPYFVNELVRYVQAGASLAEHGSSSVEPTLGLVLWK
                     RIHKLPEAAYRLLEVVAVAGQPL"
BASE COUNT          325 a          660 c          731 g          355 t
ORIGIN      
        1 gggcaaccgc tttgccggcc tcagccgcga ggtcgaagac ctgcggcagg cttgatggtg
       61 caaggctcgg aatgcggagg agagctttcg cgccgcggaa ggggtatcat agacccacca
      121 cgagcgggac atggccacca ccgtccaaga tttcccgggc accgagcggt ttgtcatcca
      181 gcagcggctt ggcgccggga gcatgggggt cgtctatcag gcctacgatc gccagcaggg
      241 cgatcgcgtc gcgttgaaga cgatcaagca tctggatccg gcggcgatct actacatcaa
      301 gcaggagttc cgcgcgctag ccgacgtgag ccatccgaac ctggtttcgc tttacgagct
      361 gttctcggag ggagagcagt gcttctttac gatgcagctg ctggagggcg tcgattttct
      421 gtcgtacgtc tgcggatacg ggccggcgac agacgccgcc aacgggagcg agccgacctc
      481 ggctccagtc ggtgcggccg agacggtggc gttcgttccc ggttcgaagg tggatgaccg
      541 catccaactc acgggcggac cgtcagcgcc tcgtccaggt tcgcccgtca gtctggaccg
      601 cttgcgaggc gccttgcagc agcttgtgga aggcgtctgt gcattgcatc gcgcgggcaa
      661 gctgcaccgc gacatcaagc cgagcaatgt cttcgtgacg cgcgaccacc gcgtggtgct
      721 gctcgatttc ggcctggcca cggaggcgac ccagcaggcc gacgaaacct ccgcgccgca
      781 cggcgtgatc ggcacggcca tctacatgtc gccggagcag gctgcgggtt tgccggcgac
      841 cagcgccagc gattggtact gcgtcggcgt cgtgctctat caggcgttga ccggtcggct
      901 gcccatcgaa gggagcaagg ggcgcgtgct gtggtacaag cagttctccg atccgctgcc
      961 gccgcgggag ctggcgcccg aaattccgga ggacctgaat gccctgtgcg tcgatctctt
     1021 gtgccgcgat ccggctgggc ggccgaccga cgagcaggtg cgagagcgcg tgggcagcgc
     1081 gccgggcgag ctggccgcat cgatcgagcc cgcccgcaag cgagcgagtc gcctggtagg
     1141 acgccagcag cacctggatg cgctggccga cgcctacgcc tcgcttcagc agggccggac
     1201 gaccgtggtc tatgtccatg gccggtcggg catgggcaaa agctcgctgt tcgagcgatt
     1261 cctcaacgat ctgcggcggg aagacgccgt catcctgagc ggccgctgtt acgagcagga
     1321 atcggtgcct tacaaggctc tcgacagcct gatcgatgcg ttgagccact accttgtgcg
     1381 cctgcccgat gctcaggtgg aagcggtgct gccgcgggac gtggcgtcgc tggcccgcgt
     1441 tttccccgtg ctgcggcgcg tggaagcggt ggcctatgcg ccgcggcgga tggccgagac
     1501 gcccgatcag caagagctgc gccggcgggc cctcgcggcg ctgcgggagc tgctgctgcg
     1561 attgggcgac cgccgaagac tggtgttggc gatcgacgac ctgcagtggg gcgatgccga
     1621 cagcgcccat ctgatcaccg agctgttgcg gccgcccgat ccgccgctgc tgttgctcct
     1681 gggctgctac cgcagcgaat atgccgaaac gagccactgc ttgcaggtcc tcttgcggcc
     1741 gcaggagcag gcgtcctcgt cggtcgagcg gcgcgagctg gcggtcgagc cactctcggc
     1801 tgacgaagcc ggcgagctcg cgctggcact gctcggcagc gattcgcccg ccgcgcaagc
     1861 cgccgctcag tcgatcgcgc gggaatcgcg cggcaatccg tatttcgtca atgagctggt
     1921 ccgttatgtc caagccgggg cgagcctggc cgaacatggg tcgtcgtccg tggagccgac
     1981 gctggggctg gtattgtgga agcgcattca caagctgccc gaagcggcgt atcggctgct
     2041 ggaagtcgtc gccgtcgccg gccagccgct g
//
LOCUS       DASUGB010000910         9253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2047378, whole genome shotgun sequence.
ACCESSION   DASUGB010000910 DASUGB010000000
VERSION     DASUGB010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047378"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFV87_17280"
     CDS             <1..265
                     /locus_tag="VFV87_17280"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX5105577.1"
                     /translation="VLRLIGFRAVESPGDELRGPCPIHGSSSAHSRSFAANVRKNTLH
                     CFKCGAKGNQLDLWVAVSKLPLFDAARDLCEQAGIDVPEVRRW"
     gene            378..869
                     /locus_tag="VFV87_17285"
     CDS             378..869
                     /locus_tag="VFV87_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105578.1"
                     /translation="MAESLPIIVCRVSTSDGVRDYVTCVSQEAAITRGLVPAAIIGVL
                     LRPVDEVASITPAVFAGNRVFIDFMHGVIARRAPSLPGLVAEARRQKDGWVYVIDQRT
                     ATPLGKVPTEDIIGAFEVHGGHVVPDSYQPNPKHLILSDSGFFGLGAELQACLLEELT
                     MEM"
     gene            1320..2567
                     /locus_tag="VFV87_17290"
     CDS             1320..2567
                     /locus_tag="VFV87_17290"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5105579.1"
                     /translation="MSDFASILPIKKTHTPADQGELASIVADAHRTGTPLYPIGGGTS
                     LDFGLPAKAAGEGLSLAGLNRIVDYPVRDMTVTVEAGVTMQVLADLLAKERQRLPIDV
                     PQSGQATIGGVIATNWNGPRRFGQGSLRDYVIGISAVDGRGMPFKGGGRVVKNVAGYD
                     FCKLLTGSLGTLGVITQTTLRLKPIPEQSVLVATALPDAAAVEKLLAALVSSKTVPAA
                     IEVLAGPAWESDPAWQMLAKPAAAPLYVVVGLEGSAAEVDSMTHQLAREWDELGIEEP
                     LVVGQSAELWRRLIEFPAAGDSPLVIKASVVPSGATAFVDAARRVEPECSIQAHAGNG
                     IVIVRFAKFPASGLSRALIGELLPAATTHQGQVVVLSNTTGSEMTHVSAWGTANPSFD
                     LMAAVKRKFDPRDILNRRRFVYS"
     gene            2570..4054
                     /locus_tag="VFV87_17295"
     CDS             2570..4054
                     /locus_tag="VFV87_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5105580.1"
                     /translation="MDTVAIESQPHQAAAPSAGGNGVPANLAGRETPAQAPAPPLMQI
                     GQPSGQPGGNIDYELFLDCVHCGLCTASCPTYVETGNENDSPRGRIYLMRAVRDERLE
                     LNHEVRRHLELCLDCRACETACPSGVQYGKLIEPFRVSMEQPASAGVQKTKTDDWFHR
                     YILFGLFPYPDKLRLSLRPARVAQTLGLDKLMINTGLWRLLPPRLGRLVTLLPKLEKP
                     APPLPEFLAAKTRRRARVALFTGCVGDVMFRQTNWATARVLQENGCDVVVPQNQVCCG
                     AIHFHAGSSDPARKMADQNAAAFDPSSVDAIIVNIAGCGSMLKDYGHHWDVGRASRLP
                     EGDGQRTEDRGQEAREQFAAKVRDVSQFLDELGLLAPPGEIRLTATYHDACHLAHAQK
                     VREQPRNLLKKVPGLKLVELPESELCCGAAGTYNLTEPEMADRLGKRKLDNIRRTGAR
                     LVVTANAGCLLQIAREARLQGEALKIMHPMDLLDLSYRREQVRF"
     gene            complement(4251..5171)
                     /locus_tag="VFV87_17300"
     CDS             complement(4251..5171)
                     /locus_tag="VFV87_17300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105581.1"
                     /translation="MPPSSGIARPPSLRARASPLPSLPSAAKPSESRLGPWHLTQVLR
                     RGALTTLYRARPAEAKGELGCYVVKTFAASGAQRDLARAMLAREAAVGQAVNHPHLAT
                     VLAADLVADQPYVVRPYLDGITLRQFLDRQRSLPLASALWIARQVAESLTVLHRAGWL
                     HGQVQPEHVIVAPSGHATLIDTSLCRRLETTECEGGDVRPGSIAYAAPETFLHRGRLT
                     TASEIYSLGISLYEMLASRPPFQAADAAQLARLHRTIAPPDLRAARPDLPPEAAYIIR
                     LMLTKEPLRRPSVAELVRWLAELEIAALAP"
     gene            5462..6598
                     /locus_tag="VFV87_17305"
     CDS             5462..6598
                     /locus_tag="VFV87_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5105582.1"
                     /translation="MFRRHSRLVSAACIASLGLLLAGCPSPSGETGGGNGASTSASGT
                     KRIVFLTNGSDPFWDACRSGLEEGAKQFEMEAAGYTVAMEVNNGKPQGQIDKLRQFAS
                     QGDVAAVAISVIQADNAAIIEEMKNLQSKGIKIITVDGDVNREKFRDARSYYIGTDNI
                     VGGRTLGTTAKAILEARSKASGGYVQFAGFTDNDNARSRMNGFKEAIGDSYTELDRMA
                     DGMDKSKARENVRNAIANHQDELAALVGIWAYNAPAIAQVVEETNTRDKYTVVTFDAQ
                     AVAIEEMAAGKIDAMVVQNPFDMGVQTVRLLKAMLADDQATIAEMFPNADQQGGDIYT
                     TGLRVVVPAEGSPVHKDLFDEKVVEYMLLPDFKAWLAKYKLTSS"
     gene            6595..7848
                     /locus_tag="VFV87_17310"
     CDS             6595..7848
                     /locus_tag="VFV87_17310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105583.1"
                     /translation="MSDSTARAWRPSLPFNELALLVAILVVVLITAAVDSNHSYLRDP
                     YTSVRDIARNVALLGIFALGAAVVIIAGGIDLSSGSMIAFSSTICASTMMLLAPQTMM
                     NMQPVGPLVAAAGIIAAICSGFLVGSLHAWLITSVRLPPFVATLATLVGLRSFARAYC
                     GYITAKTLGGSGNTEINVYDPYFKFMRDEVWIVFVPTFLVLAVFTFILLNHTVLGRHI
                     YALGGNEQAARLSGIRTESVKWFAYCFSAITASIAGVFYMADQSVASPVNQGVGYELN
                     AIAAAVVGGCSLQGGIGTVPGVIFGVIFLRVVIDSVAKLIKSGSDIYEGMIVGIVVVI
                     AVTFSQLRQIRASGGELFPGKLGWFAIPTLSAMIGLLTAMATANMPYLKGRDIWIGAA
                     AGIAALVALGAVKLLEFQRQRRSEA"
     gene            7845..>9253
                     /locus_tag="VFV87_17315"
     CDS             7845..>9253
                     /locus_tag="VFV87_17315"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX5105584.1"
                     /translation="MTAAPQPIISIQHVTKRFPGVVALEDVTLDVLPGELHAICGENG
                     AGKSTLMKILSGVLTDYEGELLLAGKPVRFKGTRDAEAAGISIIHQELNLVEQLSAAA
                     NIFLGREIRGWLVLRDDRAMEAAAARLLRELECDIHPRTLAGSLRIGDQQLIEIAKAL
                     SLQSDILIMDEPTSALTEAEVSRLYRVIERLRKRGVTILYISHKMDEIFHLADRITVL
                     RDGRLVKTLVTKETTPKEVTHLMVGREIEAVDFGSQRQAGEVLLKVENLSLPWPGHAR
                     GWRLKDVSFELRRGEVLGIAGLMGAGRTELLECLFGSAADRHEGRIELLGKPVAFRHP
                     REACRAGIALVTEDRKRLGLFAAMNVGENISICTLDKLGAAGFISRTAEWRRAADTSN
                     KLAVKSAGLRAPITSLSGGNQQKCIIGRWLLTNPKVLLLDDPTRGVDVGAKAELYRLM
                     DALCRQGLGILVTSSELPEL"
BASE COUNT         1665 a         3058 c         2994 g         1536 t
ORIGIN      
        1 cgtgctccgg ttgatcggat ttcgtgcagt tgagtcacca ggcgacgaac ttcgcgggcc
       61 atgcccgatc cacggctcgt catcggcgca cagccgctcc tttgccgcga acgtccgcaa
      121 gaacacgttg cactgcttca agtgcggcgc aaaggggaac cagctcgacc tgtgggtcgc
      181 cgtctccaag ctgccgctgt tcgatgcggc ccgcgacctg tgcgagcaag caggcattga
      241 cgtgcccgag gtccggcggt ggtagagaac gcggatagca atgaaacaat ccagttcaag
      301 ttggagcgtt ccgcatctca gccaagtacg ggctcctctt ctctgattac cgacggtgtt
      361 gcccaaagga tttacaagtg gcggagtcgt tgccaatcat cgtgtgccgc gtctcgacgt
      421 ctgatggcgt gagggactac gtcacgtgcg tatcccagga ggcggcaatc acgcgaggct
      481 tggtgcccgc ggcgatcatc ggcgtgctgc tacgccccgt cgacgaggtg gcttccatca
      541 cgcccgccgt gtttgctggc aatcgcgtat tcatagactt tatgcacggg gtcatcgccc
      601 gtcgcgcacc cagcttgccg gggctggttg ccgaagcgcg gcggcaaaag gacggctggg
      661 tgtacgtcat cgaccagcgg accgcgacgc cccttgggaa agtcccgacc gaggacatca
      721 tcggggcgtt tgaggtgcat ggcggacatg tcgtgccaga ctcttatcag cccaacccca
      781 aacacctgat actgtcagac agcggcttct tcggtttggg agccgaattg caggcatgtt
      841 tgctggagga actcacgatg gagatgtagc cgaagtattc gcgaccggtt cctcgtatcc
      901 atgaagctga gtggtaagca tcagagaaga ggagcccgta ttcggctggg cgagatgcca
      961 atgcttggaa cggccgggga ctttaccgtc tcctacagcg gcttcacgtc gtcgattaga
     1021 atcgagacga tgagcacgtc ttccgaatca tcgaagacgc acaggataag tcctgacgat
     1081 ccgcgccacc cggaatatgg cgcttacctg gcgtggctcc ggagactgac gatggaggag
     1141 cgcggcagga tgatcctgga gaaatgccgc gaagccgccg aggaggaaca cgcgcgaatc
     1201 gcatcgggct tgccgcccac aaccccccca gccgtggccg gcatcgacgt gggacctgtt
     1261 gcgccgcctg gctcgccagt cgcgacacga ctccagccgc gcaagtggcg tgcagcccca
     1321 tgagtgactt cgccagcatc ttgcctatca agaaaacgca cacgcccgcc gatcagggcg
     1381 agctggcgtc gatcgtcgcg gatgcgcacc gaaccggcac gccgctctat ccgattggcg
     1441 gcgggacgag tctggatttt ggcttgccgg cgaaggcggc gggggaggga ttgtcgctgg
     1501 cggggctgaa tcggatcgtc gattatccgg tgcgcgacat gacggtcacc gttgaagcgg
     1561 gcgtgacgat gcaggtgctg gcggacctgc tcgccaagga gcgccaacgg ctgccgatcg
     1621 atgtgccgca atccggacag gcgacgatcg gcggcgtgat tgccacgaac tggaacgggc
     1681 cgcgacgatt cggccagggc tcgctgcgcg actacgtgat cggcatcagc gccgtcgatg
     1741 gccgcggcat gccgttcaaa gggggcgggc gcgtcgtcaa gaacgtcgcc ggctacgact
     1801 tctgcaaatt gttgaccggc tcgctcggga ccctcggcgt gattacgcag acgacactgc
     1861 ggctcaagcc gattccggaa cagtcggtgc tcgtcgcgac tgccctgccc gatgccgcgg
     1921 cggtcgagaa gctgctggcg gcgctcgtgt cgtcgaagac cgtccccgcc gccatcgaag
     1981 tgctggctgg tcccgcgtgg gaaagcgatc ctgcttggca gatgctcgcc aagcccgccg
     2041 ccgcgccgct gtacgtcgtg gtcggcctcg aaggcagcgc ggccgaagtc gattcgatga
     2101 cgcaccagct cgcgcgcgaa tgggatgagc tgggcatcga agaaccgctc gtggtcggcc
     2161 aatcggctga actgtggcgg cggctgatcg agtttccagc cgcgggcgat tcgccgctgg
     2221 tgatcaaggc cagcgtcgtt cccagcggcg cgaccgcgtt tgtcgacgcc gcccgacgcg
     2281 tagagcccga gtgctcgatc caggcccacg ctggaaacgg catcgtgatc gtgcggttcg
     2341 ccaaattccc cgccagcggc ctgtcgcggg ccctcatcgg cgagttgctc cccgcggcca
     2401 ccacgcacca aggtcaagtt gtcgtgctct ccaacaccac cggctccgaa atgacgcacg
     2461 tcagtgcctg gggaacggcc aatccgtcgt tcgatctgat ggctgccgtc aaacgcaagt
     2521 tcgatccgcg cgatatactg aaccgccgga ggtttgttta cagctagtca tggacaccgt
     2581 cgccatcgaa tcgcagccgc atcaagctgc cgctccatcg gctggtggca acggtgtgcc
     2641 cgcgaacctc gccgggaggg aaacgccggc acaggcgccc gccccgccgc tgatgcagat
     2701 tggtcagccc agtggccagc cgggcgggaa catcgactac gagctgttcc tcgactgcgt
     2761 ccactgcggc ctctgcactg cgtcgtgccc gacgtacgtc gagacgggga atgaaaacga
     2821 cagcccgcgc ggacgcattt acctgatgcg ggccgtccgc gacgagcggc tggagctgaa
     2881 tcacgaagtc cgccggcatc tggagctctg tctcgactgc cgggcctgcg aaaccgcgtg
     2941 cccctccggc gtgcaatacg gcaagctgat cgagccgttc cgcgtgagca tggagcaacc
     3001 tgcgtcggcc ggcgtgcaga agacgaagac cgacgactgg ttccaccgct acatcctgtt
     3061 cgggctgttt ccctatcccg acaagctgcg gctgtcgctg cggccggccc gcgttgccca
     3121 gacgctgggc ctcgacaagc tcatgatcaa cacgggcctg tggcggctct tgccgccgcg
     3181 actgggccgg ttggtcactc tgctgccgaa gctcgaaaag cccgccccgc cgctgccgga
     3241 gtttctcgcc gccaagacgc gccggcgggc ccgcgtggcc ctcttcacgg gctgcgtggg
     3301 cgatgtgatg ttccgccaga cgaattgggc caccgcccgg gtcctgcaag agaacggctg
     3361 cgacgtcgtc gtcccgcaaa accaggtctg ctgcggcgcg atccactttc acgccggcag
     3421 cagcgatccg gcccgcaaaa tggccgacca gaacgccgcc gctttcgatc cgtcgagcgt
     3481 cgatgccatc atcgtcaaca tcgccggctg cgggtcgatg ctgaaggact atgggcatca
     3541 ttgggacgtg gggcgggcgt ctcgcctgcc cgaaggagat ggacagagga cagaggacag
     3601 aggacaggag gctcgcgagc agtttgccgc caaggttcgc gatgtgagcc agttcctgga
     3661 tgaactcggc cttctcgcgc cgccgggtga aatccgcctc acggccacgt atcacgacgc
     3721 ttgccacctg gctcacgccc aaaaggtccg cgagcagccg cggaacttgc tgaagaaagt
     3781 tcccggcctc aagctcgtcg agctgccgga atcggagctg tgctgcggcg cggcgggcac
     3841 gtacaacctg actgagccgg aaatggccga tcgcctcggc aagcggaagc tcgacaacat
     3901 ccgccgcacc ggcgcgcggc tggtcgtcac tgccaacgcc ggctgtctcc tgcaaattgc
     3961 ccgcgaagcc cgcctgcaag gcgaagccct caagatcatg caccccatgg acctgctcga
     4021 cctcagctac cgccgcgagc aggtgcggtt ctagtcaggc atcacgttcc gcgtgatgac
     4081 gcgactcgcg cagcgagtcg ccaaccgtag gcttctcgct ccgcgagaag cgatcgactc
     4141 gccttacgaa gcagcgccgc gcactaggaa agccgactag gtagcagtta tcgtggtggc
     4201 gtgaaggccg cgcacgaaat gcgcctattg cgactcgcgg agcgagtcgc ctacggtgcg
     4261 agcgccgcga tctccagttc agccagccag cgcaccaatt ccgcgacgct cggccgccgg
     4321 agcggttcct tcgtaagcat cagacgaatt atgtacgcgg cttcgggcgg caggtcgggc
     4381 cgcgcggcgc gcaggtcggg cggtgcgatg gtgcggtgta gcctcgcgag ctgcgccgcg
     4441 tctgctgctt ggaacggcgg ccggctggcg agcatttcgt aaaggctgat gccgagcgag
     4501 tagatttcgc tggcggtggt cagtcgcccg cggtgcagga atgtctcggg cgcggcgtag
     4561 gcgatcgagc cggggcgcac atcgccgccc tcgcattctg tcgtctccag cctccggcag
     4621 agggaggtgt cgatgagcgt cgcgtggccg gaaggcgcaa cgatgacgtg ctcgggctgg
     4681 acttgcccgt gcagccagcc ggcgcgatgc agcaccgtga gcgattcagc aacttgccgg
     4741 gcaatccaga gcgcagaggc aagcggcaat gatcgctggc gatcgaggaa ctgccggagc
     4801 gtgatgccgt ccaggtaggg ccgcaccacg tacggctgat cggcgacgag atcagcggcc
     4861 aggacggtcg ccaaatgcgg atggttcacg gcttggccga cggctgcttc gcgggcgagc
     4921 atcgcacggg ctaggtcccg ctgtgcgccg ctagccgcga aggtcttgac gacgtagcag
     4981 ccgagttcgc ccttggcttc cgccggccgc gcgcggtaca gagtcgtcag cgcaccgcgg
     5041 cgcagcacct gcgtcagatg ccacggcccc aaccggcttt ccgacggctt cgcggcactt
     5101 ggcagagagg gcaggggact agcgcgagcg cggaggctcg gcgggcgggc gatgccggaa
     5161 gagggtggca tgatggggta gaaaggagtg cgccatttcg ccttatcggg ccgcagtacg
     5221 ctccgcgatg aagcctccgc cgccagccgc cacagcttgt catgtcgcgc aagcagaaaa
     5281 gagcgaacca gccgcgactt gcaagcccct cgcggcaaaa ctagaatccg aactttcgac
     5341 agcgcaacac cttgcgcctc ctccggctgt aggatggacc tctggtccgt ccgtgttggt
     5401 aggtaggatg gacctcctgg cccgtccgtg ttgaactgtc ctttatcagg ggttcctctc
     5461 catgttccgc cggcactccc gcctcgtttc cgcggcctgt atcgcttcgc tgggactcct
     5521 cctcgccggc tgccccagcc ccagcggcga aacgggcggc ggaaacggcg cgtcaacgag
     5581 cgccagcgga accaagcgca tcgtcttcct caccaatggc agcgacccct tctgggacgc
     5641 ctgccgcagc ggcctggaag aaggggccaa gcaattcgag atggaagccg ccggctacac
     5701 cgtcgccatg gaggtcaaca acggcaagcc gcagggccag atcgacaagc tccgccagtt
     5761 cgccagccag ggcgatgtgg ccgctgtcgc catctcggtc attcaggccg acaacgcggc
     5821 gatcatcgaa gaaatgaaaa acctgcagag caaggggatc aagatcatca cggtcgatgg
     5881 cgacgtgaac cgagaaaaat tccgcgacgc ccgctcgtac tacatcggca cagacaacat
     5941 tgtcggcggc cgcaccctcg gcacgacggc caaggccatt ctcgaagcgc gcagcaaggc
     6001 cagtggcggc tacgtccaat tcgccggctt caccgacaac gacaacgccc gcagccgcat
     6061 gaacggcttt aaagaggcga tcggcgactc ctacaccgag ctcgatcgca tggccgacgg
     6121 gatggacaaa tcgaaggccc gcgaaaacgt ccgcaacgcc atcgccaatc accaggatga
     6181 gctggccgcc ctcgtcggca tctgggctta caacgccccg gccatcgccc aggtcgtcga
     6241 agagaccaac acgcgcgaca agtacacggt cgtcacgttc gacgcccagg ccgtcgccat
     6301 cgaagaaatg gctgccggca agatcgacgc gatggtcgtg caaaacccgt tcgacatggg
     6361 cgtgcagacc gtccgcctgc tgaaggcgat gctggcggac gatcaggcga cgatcgccga
     6421 aatgttcccg aatgccgatc agcagggcgg cgacatctac acgaccggcc tccgcgtcgt
     6481 cgtccccgcc gagggctcgc ccgtgcacaa ggatctgttc gacgagaaag tcgtcgagta
     6541 catgctgctc cctgatttca aggcctggct ggccaaatac aaactcacca gctcatgagc
     6601 gactcgacag ccagggcctg gcgcccttcg ctccctttca atgagctggc cctcctggtg
     6661 gcgattctcg tcgtcgtgct gatcacggcg gcggtcgatt ccaaccacag ctacctgcgc
     6721 gacccgtaca ccagcgtccg cgacatcgcc cgcaacgtgg ccctgctcgg catcttcgcc
     6781 ctcggggcgg cggtcgtcat catcgccggc ggcatcgacc tctcgtccgg ctcgatgatc
     6841 gccttcagca gcacgatctg cgccagcacg atgatgctgc tcgcgccgca gacgatgatg
     6901 aacatgcagc cggtcgggcc gctcgtggcg gcggccggca tcatcgcggc gatctgctcc
     6961 gggtttctcg tcggctcgct gcacgcctgg ctcatcactt cggtgcgcct gccgccgttt
     7021 gtggccacgc tcgccacgct cgtcggcctg cgcagctttg cccgggccta ctgcggctat
     7081 atcacggcca aaacgctcgg cggcagcggc aacaccgaga tcaacgtcta tgacccgtac
     7141 ttcaagttca tgcgcgacga ggtgtggatc gtcttcgtcc cgacattcct cgtcctggcg
     7201 gtcttcacgt tcatcctgct caaccacacg gtcctcggcc ggcacattta cgccctgggc
     7261 ggcaacgagc aggcggcccg cctgtcgggc attcgcaccg aatcggtcaa gtggttcgcc
     7321 tattgcttca gtgcgatcac ggcatcgatc gccggcgtct tttacatggc cgatcaaagc
     7381 gtcgcctcgc ccgtgaacca gggcgtcggc tacgagctga acgccatcgc tgcggcggtc
     7441 gtcggcggct gcagcctgca agggggcatc ggcacggtgc cgggcgtgat tttcggcgtc
     7501 atcttcctcc gcgtcgtcat cgactcggtc gccaagctga tcaaatcggg ctccgacatc
     7561 tacgaaggga tgatcgtcgg catcgtcgtc gtgattgccg tcacgttcag ccaactccgc
     7621 cagattcgcg ccagcggcgg ggaactcttc cccggcaagc tcggctggtt cgcgatcccc
     7681 acgctctcgg ccatgatcgg cctgctcacc gcgatggcca cggccaacat gccgtacctc
     7741 aaaggccgcg acatctggat tggcgctgcc gccgggatcg ccgccctcgt cgccctcggc
     7801 gcggtcaagc tcctggaatt ccagcgccag cgcaggagcg aagcatgaca gccgctccgc
     7861 agcccatcat ctcgatccag cacgtcacca agcgcttccc cggcgtggtg gcgctggaag
     7921 acgttacgct cgatgtgctg ccgggggaac tgcacgcgat ttgtggcgag aacggcgcgg
     7981 gcaagagcac gctgatgaag atcctctcgg gcgtgctgac cgattacgag ggcgaactgc
     8041 tcctggccgg taagccggtg cggttcaagg gcacgcgcga cgccgaagcg gcaggcatca
     8101 gcatcattca ccaggagttg aacctcgtcg agcagctctc ggcggcggcc aatatcttcc
     8161 tgggacgcga aatccgcggc tggctggtcc tgcgcgacga ccgggcgatg gaagcggccg
     8221 cggcgcgcct gctgcgcgag ctggaatgcg acattcatcc gcggacgctc gccggcagcc
     8281 tgcgcatcgg cgaccagcag ctcatcgaaa tcgccaaggc gctctcgctg caaagcgaca
     8341 tcctcatcat ggacgagcca accagcgccc tcaccgaagc cgaggtcagc cggctgtatc
     8401 gcgtgatcga gcggctgcgg aagcggggcg tgacgatcct ctacatctcg cacaagatgg
     8461 acgagatctt ccacctggcc gaccggatca ccgtcctccg cgacggccgg ctcgtcaaaa
     8521 cgctcgtcac caaggagacg acgccgaagg aagtcacgca cctgatggtc ggccgcgaga
     8581 tcgaggccgt cgatttcggc agccagcggc aggcgggcga ggtgctgctc aaggtcgaaa
     8641 acctctcgct cccctggccg ggccacgccc gcggctggcg gctgaaggat gtgtcgttcg
     8701 agctgcggcg gggcgaagtg ctgggcatcg ccggcctgat gggggccggc cgcaccgagc
     8761 tgctcgaatg cctgttcggg tcggcagccg accggcacga ggggcggatc gagctgctcg
     8821 gcaagccggt cgcgttccgc cacccgcgcg aggcctgccg ggccggcatc gccctggtca
     8881 ccgaggaccg caagcggctg gggctcttcg ccgcgatgaa cgtgggcgaa aacatctcga
     8941 tctgcacgct cgacaagctc ggggccgccg gcttcatcag ccgcaccgcc gagtggagaa
     9001 gagcggcgga cacgtcgaac aagctcgccg tcaaatcggc cggcctgcgg gcccccatca
     9061 ccagcctcag cggcggcaac cagcagaagt gcatcatcgg ccgctggctg ctgaccaatc
     9121 cgaaagtgct gttgctcgac gatcccacgc gcggcgtcga cgtgggcgcc aaggccgagc
     9181 tgtaccgcct gatggatgcg ctgtgccgcc agggcctggg catcctggtc acttcgagcg
     9241 aactgcccga gct
//
LOCUS       DASUGB010000911         3711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_796531, whole genome shotgun sequence.
ACCESSION   DASUGB010000911 DASUGB010000000
VERSION     DASUGB010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796531"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFV87_17320"
     CDS             <1..759
                     /locus_tag="VFV87_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX5105585.1"
                     /translation="LEIGGQTIRLAGMCKGAGMIGPHMATMLAILTTDAALTPADAQA
                     VLKAAASESFNCISVEGHMSTNDTVLLLASGAASGTEDSGQRTADNPQSAIRNPQLLA
                     AFQAALTDLSIELARQIPDDGEGASHLIVLDVKGCKTRDDARRIAQTMANSALVKTAV
                     SGGDPNWGRIVSAAGYAGVPFDPAAVDLAVNGCTLYERGAPVPFDAKTVSTSIKSHRE
                     TSLVLTLREGPASIRFWTSDLNADYVRFNADYTT"
     gene            848..>3711
                     /locus_tag="VFV87_17325"
     CDS             848..>3711
                     /locus_tag="VFV87_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105586.1"
                     /translation="MQRFSSEPLQAVILPDPPPQPPPSGAWKTWCLAIGAFGLFIVCS
                     GAAFWGLGKLVNFGVDASPKGSLVYSWETPEEHRVNVTAAMNVKTTGCTAAELRELNR
                     FFTRVIDALAASDKDEFRELVDLSAFAHRASLHPAAVAAGGLDRGQLKDQLEYDLEGP
                     SGWSRFSIIRVEHGKIPDEALVYVVFESDEQQPAPYRWWLRRSARVWNICDWELIDLG
                     YSETARWALVRAIEDDPDNGSYWQAAAAIERSHDKAAAGQRGAAQAELRDAQTKRLPA
                     AIHDMTQIELAIAWDYAGRPDLALTACDRVKNASSEIGVAYLRAHSQVALDVPDKALT
                     ALAEYRQMAGCHPELLALEADALGAQGKRANAAECWWEVLRLMPENHEALFDFCRLAD
                     DSRRKAIKEILARSRRPTDRALEIGIAAIQRDEYAAAEAILDYLRQTAPGSAAIEQLT
                     AQQLDDDEQYVAAAEHYLAAFKLESDPDNKRQLLREYLGSMALGGKAVEGYLAADDKA
                     EAFDLLTDGYEYDEAYVRDDQLSALIAEHRQHVAEDATARLDFLEGRRLLNQGDFDAA
                     EQAFLKIETSDVEPDLVEAIRSGRLEALYRQGKLKEAYEADDQATAETFRELAWLAVG
                     DDNWDALGKLLELHLTSNSGDPWLDYFTTRREQAYRNYTTALAAAVRGEASADEQLKP
                     LLMRLKTELFVQSDNISQAYASGGPPQEAFLRIAGQLTDQGDWNRLLQAAELHSAAAP
                     GEIAGLYYATKARWNLGRHQQLVQNLTPWPKERLQNADQAWNVEIADLLVRGWLRLGN
                     IEEARKAAEQARDDLGLELPLVAVELAAGDREKVKTLLANPLIGRELFQRQLQHDSDF
                     AALLSDSEFAEIRRRYAHQRPNEYGQRNASIVLLLRKPAEESRVKEDFEHEVASAMGG
                     PVRSLGQSAGETHRQSLIADLPAGTLAFTYASRPYSTSAGL"
BASE COUNT          690 a         1236 c         1182 g          603 t
ORIGIN      
        1 ctggaaatcg gcgggcagac gatccgcctc gccggcatgt gcaaaggagc cggcatgatc
       61 ggcccacaca tggcgacgat gctggcgatc ctcacgaccg acgccgcgct cacccccgcc
      121 gacgcccaag ccgtcctcaa agccgccgcc agtgaaagct tcaactgcat cagcgtcgaa
      181 ggacacatga gcaccaacga tacggtgctg ctgctggcca gcggagcggc aagcggaaca
      241 gaggacagcg gacagaggac agcggacaat ccgcaatccg caatccgcaa tccgcaattg
      301 ctcgccgctt tccaagcggc gctcaccgac ctctcgatcg agctcgcccg ccagattccc
      361 gacgacggcg aaggcgcctc gcatctgatc gtcctcgatg taaaaggttg taaaactcgc
      421 gacgatgccc gccgcatcgc ccagacgatg gccaacagcg ccctcgtcaa aacggctgtt
      481 tccggcggcg accccaactg gggccgcatc gtctccgccg cgggctatgc cggcgtcccg
      541 ttcgatcccg ccgccgtcga tctggcggtc aacggctgca cgctctacga gcgcggcgcg
      601 ccggtgccgt tcgacgccaa gaccgtctcc acctcgatca aatcccatcg cgaaaccagc
      661 ctcgtgctca cgctccgcga aggtcccgcc tcgatccgct tctggaccag cgacctcaac
      721 gccgactacg tccgcttcaa tgcggactac acgacatagg ccgcgtgggc gttgaggatg
      781 ccgggtactt tggcgataat cgcggctgtc gtgtttcgcg tctcttcccg cgccgtctga
      841 ggcccccgtg cagcgttttt cgtccgagcc gcttcaggcc gtcattctgc cggacccgcc
      901 gccgcagccg ccgccgagcg gggcgtggaa gacctggtgc ctggcgatcg gtgcattcgg
      961 cctgttcatc gtctgctcgg gggcggcctt ctggggcctg ggcaagctcg tgaattttgg
     1021 cgtggacgcc agccccaaag gctcgctcgt ctattcctgg gaaacacccg aggagcaccg
     1081 cgtcaacgtt acagccgcga tgaacgtcaa aacgaccggc tgcacggcgg cggagctgcg
     1141 cgagctgaac cgctttttca cgcgggtcat cgatgcgctg gccgcgtcgg acaaggacga
     1201 gttccgcgag ctggtcgatc tcagcgcttt cgcccaccgg gccagcctgc accccgccgc
     1261 ggtcgctgct ggcgggctcg atcgcggcca actgaaagac cagctcgaat acgatttgga
     1321 aggcccgagc ggctggtcgc ggttctcgat catccgcgtc gagcacggca agattcccga
     1381 cgaggccctg gtgtatgtcg tcttcgaaag cgacgagcag cagccggcgc cctaccgctg
     1441 gtggctgcgg cgtagcgcgc gcgtctggaa catttgcgac tgggaattga tcgacctggg
     1501 ctacagcgag actgcccggt gggccctggt ccgggccatt gaagatgatc ccgacaatgg
     1561 atcgtactgg caggccgccg cggcgatcga gcgttcgcac gacaaggccg ctgctggaca
     1621 gcgtggcgcc gcccaagccg aactgcgcga tgcccagacc aagcgcctgc cggctgcgat
     1681 tcacgacatg acgcagatcg agctggcgat tgcctgggat tatgccgggc ggcccgattt
     1741 ggccctgacg gcctgcgatc gcgtcaaaaa tgccagcagc gagattggag tcgcgtatct
     1801 ccgggcccac tcccaggttg cgctcgatgt gcccgacaag gcgctcaccg cgctggccga
     1861 atatcgccag atggccggct gtcatccgga gttgctggcg ctcgaggcgg acgcgctcgg
     1921 agcccaggga aagcgggcca acgccgctga gtgctggtgg gaggtcttgc ggctgatgcc
     1981 ggagaatcac gaggcgctgt tcgatttttg ccgcctggcg gacgactcgc ggcgcaaggc
     2041 gatcaaggag attctcgcca ggagccgccg gccgacggac cgggcgctgg agatcggcat
     2101 cgcggcaatt cagcgggacg aatacgccgc ggcggaagcg attctcgact acctccgcca
     2161 aaccgcgccc ggttccgccg caatcgagca gctcactgcc cagcagctcg acgacgacga
     2221 acagtacgtc gccgcggccg aacattatct cgcggcgttc aagctcgaaa gcgatccgga
     2281 caacaaacgg cagcttctgc gggagtattt gggttcgatg gccctgggtg gcaaggccgt
     2341 ggaaggctat ctcgcggcag acgacaaggc ggaggcgttc gacttgctca cagacggcta
     2401 cgagtatgac gaggcctacg ttcgcgatga ccagctcagc gccttgatcg ccgagcaccg
     2461 ccaacacgtg gcggaagatg cgacagcccg gcttgatttt ctggagggcc gccgtttgct
     2521 caatcagggc gattttgacg ccgccgagca ggcgtttctc aaaatcgaaa ccagcgacgt
     2581 cgaacccgac ctggtcgagg cgatccgctc aggccgactc gaggcgctct accggcaggg
     2641 gaagctgaag gaagcgtacg aggctgacga ccaggccacg gccgaaacat ttcgcgagct
     2701 cgcctggctg gcggtgggcg acgacaactg ggacgcgctt ggcaagctcc tcgagctgca
     2761 cctgacatcg aactccggcg atccctggct cgattatttc acgactcgcc gcgagcaggc
     2821 ttatcgcaac tacaccacgg ccctcgccgc ggccgtacgg ggcgaggcca gcgccgacga
     2881 gcagctcaaa ccgctgctaa tgcggctgaa gacggagttg ttcgtccagt cagacaacat
     2941 cagccaggcc tatgccagcg gcggcccgcc gcaggaagca ttcttgcgga tcgccggcca
     3001 attgaccgac cagggcgatt ggaaccgctt gctgcaggct gccgagctgc attccgctgc
     3061 ggcgccgggc gagatcgcgg gactttacta tgcgaccaag gcccgctgga acttgggccg
     3121 ccaccagcag ctcgtgcaga acctcacacc ctggccgaaa gagcgcctgc aaaatgccga
     3181 tcaagcctgg aatgtcgaaa tcgccgacct gcttgtccgc ggctggctgc ggctgggtaa
     3241 tatcgaagaa gcccgcaaag cagccgaaca ggcgcgggac gatctgggcc tggagctgcc
     3301 gctggtggcg gtcgaactgg ccgcgggcga tcgcgagaag gtcaaaacgc tgctcgccaa
     3361 cccgctcatc ggccgcgagc tcttccagcg gcagcttcag cacgattccg attttgcggc
     3421 gctgctttcc gattcggagt ttgccgaaat ccgccgccgc tacgctcacc agcgtccgaa
     3481 cgaatatggc caacgaaatg cctcgatcgt gctgctactc cgcaagccgg cggaggaatc
     3541 gcgtgtaaag gaggatttcg agcacgaggt tgcgtcggcc atgggcgggc cggtgcggtc
     3601 gctcggccag agcgcgggcg aaacccaccg gcagagcctc atcgccgatc ttccggccgg
     3661 cacgctcgcc ttcacgtatg ccagccgccc ctattctacg tctgcaggcc t
//
LOCUS       DASUGB010000912         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_936369, whole genome shotgun sequence.
ACCESSION   DASUGB010000912 DASUGB010000000
VERSION     DASUGB010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936369"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFV87_17330"
     CDS             <1..1171
                     /locus_tag="VFV87_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105587.1"
                     /translation="IITEEYERTEPAKAEAQSSIAEAAAPSLLANGGFEKIGSVSTDP
                     DDWFATRLPTTAGHYVLAASQSVSRSGKCSVVVEIADNHPETPVHYNWTAVAKGWKSG
                     ETYELSGWIKTENAKSPAIIMAQLWSEGSKPKIIGGATTQLVQPVTGTKDWTRVSTQL
                     KVPEGTAELRIRAILSSQENRGAKAWFDDISLVKIGRSPVESLKDGPGKSRAANAGTA
                     SLISNGGFEVVQANSSDPQEWFGTRIPQTAGHYLVASSADSHRGNRSAVVEIGDNHPD
                     RPVAYNWTTEANGWRAGETYELSGWIKTESAKNPAFIMAQFWAGSGTEKKMLGVAAAQ
                     RAFPVTGTSDWTRVSTRLTVPEGTDVVRIRAGLSSQDNRGAKAWIDDISLVKVPN"
     gene            complement(1416..1970)
                     /locus_tag="VFV87_17335"
     CDS             complement(1416..1970)
                     /locus_tag="VFV87_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105588.1"
                     /translation="MKKKPAGRRPTPGKAEATAGTQSVAQSVAARRTAEAQVRALIAR
                     LAPQHVLLISAMRRSLRKRLPTAHEVVYEYRDWFVISFSPSERGYEGVLAIRGSASGI
                     KLFFNRGKELPDPENLLQGSGSAQARFIGIEAASTLARPAIARLIDQSIARNRVPFAP
                     TGRGSVVIRATTAKNKQSRPRRPA"
BASE COUNT          417 a          567 c          679 g          373 t
ORIGIN      
        1 catcatcacg gaggaatatg agaggacgga gccagccaag gccgaggctc agtcgagcat
       61 cgccgaggca gccgctccat cgctattggc gaacggcgga tttgagaaga tcggcagcgt
      121 ttcgactgac cctgacgatt ggttcgccac gcgtttgccg acgacggcag gccactatgt
      181 gctggccgcg tcgcagtcgg tctctcgtag cggtaagtgc agcgttgttg tggagatcgc
      241 cgacaatcat cctgaaactc cagttcacta caattggaca gcggttgcca agggctggaa
      301 gtccggtgaa acgtatgagt tgtcaggttg gattaaaacg gaaaacgcca aaagtcccgc
      361 gatcatcatg gcgcagcttt ggagcgaagg atcaaaacca aagatcatcg gcggcgcaac
      421 aacccaattg gttcaaccgg tgacaggcac aaaggattgg acgcgggtga gcacgcagtt
      481 gaaggtccct gaaggcacag ccgagttgcg gattcgtgcc attttgtcct ctcaagagaa
      541 tcgaggagcg aaggcctggt tcgatgacat ttcgctcgtc aagattgggc ggagccctgt
      601 tgaatcgttg aaggatggac ccggcaagag tcgggcggct aacgcgggaa ccgcatcgct
      661 catttcaaac ggagggtttg aggtggtcca ggcgaattcg agcgacccgc aggaatggtt
      721 cggcacgcgg attccgcaga cagccggcca ttatctggtc gcgtcgtctg cggactccca
      781 ccgcggcaat cggagcgcgg tcgtggagat cggagataac caccccgaca ggcccgtcgc
      841 ttataactgg actaccgagg ccaacggatg gagagccggc gaaacgtacg agttgtcggg
      901 ctggataaag actgagagtg ctaagaatcc agcgttcatc atggcgcagt tctgggcggg
      961 cagtggaact gaaaagaaaa tgctcggtgt cgccgcggcc cagcgggcct ttccagtcac
     1021 cggcactagt gactggacac gagtaagcac tcggctgaca gtccctgaag gcaccgatgt
     1081 agtacggatt cgagctgggc tatcgtctca ggacaatcga ggagcgaagg cttggatcga
     1141 cgacatttcg ctggtcaaag tgccgaattg agtgcctcag aaacgcagtt cctggtggtt
     1201 catgcgggag ctcgagcagt cggcgactcg ccagggaatg ttcctacgct gggaaccctt
     1261 gacggagaac gcggcagcgc gaccttaatt gctgggcgct agcgaccggc ctgctggaca
     1321 cgggcacgat gcccgggcca cacggccgga gaagtgattg gcggcgcgag ccgtggtttg
     1381 cggcgcgaag caccgccagc cggccgtggt tcgcttcagg cggggcgacg cggccgactc
     1441 tgcttgttct tggccgttgt cgcacgaatg accaccgacc cgcggccggt gggcgcgaac
     1501 ggcacgcgat ttcgagcgat ggactggtcg atcaggcgcg cgatcgccgg gcgagcgagg
     1561 gtggatgcgg cctcgatgcc aataaaccgc gcttgggcgc tgccggaccc ctgcaacagg
     1621 ttctcaggat ccggcagttc cttcccacga ttgaagaaca gctttatgcc gctggcgctc
     1681 ccgcggatgg caagaacgcc ctcgtacccg cgctcgctgg gcgaaaagct gatgacgaac
     1741 cagtcgcggt attcgtacac gacctcatgg gcagtgggca gacgcttgcg cagtgaccgc
     1801 cgcatggcgc tgatgagcag cacgtgctgg ggcgcgagcc tggcgatcaa cgcgcgcacc
     1861 tgggcctctg ccgttcgccg cgcggcaaca gactgagcaa ctgactgagt gcctgcggtg
     1921 gcttcggcct taccgggggt gggacggcgg ccagcgggct ttttcttcat gctcgtgatt
     1981 gctccatggg attgtccttg attaacgacg gggccctcgc tgacgcttcg ggttgg
//
LOCUS       DASUGB010000913         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1632043, whole genome shotgun sequence.
ACCESSION   DASUGB010000913 DASUGB010000000
VERSION     DASUGB010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632043"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2236
                     /locus_tag="VFV87_17340"
     CDS             <1..2236
                     /locus_tag="VFV87_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5105589.1"
                     /translation="MPPMQDKLADAKLALISKWIEGGAPENAGSKVAIKKNPLAAVAS
                     GSIGKPEGPPPMPESLLKQPVLYTDKPGQITALACSPWAPLVAVAGQKQVAIYNSDNA
                     QLVGILPFPEGIPHVVRFSRNGAVLLVAGGRGGHSGCVVLFDVKTGKRMARLGEETDA
                     ILAADINATHTLVALGGPSKVVRIFSVQTGEMVGEIRKHTDWIYGVEFSPDGVLLATS
                     DRSGGLFVWEADTAREYLNLRGHNGAVYCVSWRMDSNILASGGEDTTVKLWEMNDGNA
                     IKSWGAHGGGAFCVSYAHDGRLVTAGRDNTVKSWNGDGAALKTFPGFPEAGMRCAFTY
                     DGKRVVGGDWLGNVKVWDAEQAVEAYTLAANPPTLVMRVDAAKADLAARQAAAAAAAG
                     EYDAAVKLAAEKDAALKAAGEKHTAAVAAAQKTEADRVAAVKASEVAAAAVKPAMDAA
                     AAAQTAAVTAGNEKAAADKAAGDKAAATKVVADQAASERAAADKLAAEKSAEAPAAAE
                     KAAATAVVAAQADAERAQLAVVAAQKTEAATKTSNALTAANAKLAEAQNAATTAAKQV
                     ETQTAAAKAAADAVPVAKAEVDKATQEKAAADAAAAAKKQAADAAAAAAAASQAALAQ
                     AEADKVAFEQMAGKLSAALDEAGKKITAQQAALTQAEAESKTLGDAVAAKTAVSKDLT
                     DKIAALQAELAKAQADQKAAEEAAAAKAKAAADAGAALAEAQAAAEAAAAEQKAFAEA
                     YAAK"
     gene            complement(2319..2549)
                     /locus_tag="VFV87_17345"
     CDS             complement(2319..2549)
                     /locus_tag="VFV87_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105590.1"
                     /translation="MERCKNVFEAILKYGHDEEWSPDPHQKFEATDLPAGSPEKIEIL
                     RKRLELGLPLFHENDRYDYNGLVGAIRPRDAM"
     gene            3034..3453
                     /locus_tag="VFV87_17350"
     CDS             3034..3453
                     /locus_tag="VFV87_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5105591.1"
                     /translation="MTWIQRQMSLPARKRGFHLVTREVVEALPEIRMYRVGLLHVFIQ
                     HTSAALTINENADADVRHDLESSLNAIAPEEFPYVHTIEGPDDMPAHVKASLMGSSVS
                     IPIADGQLLLGTWQGIYLCEHRNHGGNRKLVLTLWGE"
     gene            complement(3527..>4110)
                     /locus_tag="VFV87_17355"
     CDS             complement(3527..>4110)
                     /locus_tag="VFV87_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105592.1"
                     /translation="LPTSRSRCQIVPFRPLTQSDVEELLLQCGLCEDRAVAQQAAARS
                     GGSVAHAAQWCEPELIEFRSQLLDALANPEMELLQAAKLVGQFVEGAGKESAAKRDRL
                     RLAVSLAEEFYRSLLLGDAALCDAQDKPLTSAIARASRWIAGQESAAACLDVCLDAYA
                     HIDSNVNQATFIEWWLDELSQAARRGAGFQPAR"
BASE COUNT          813 a         1280 c         1355 g          662 t
ORIGIN      
        1 gatgccgccg atgcaggaca agctggccga cgccaagctg gccctgatca gcaagtggat
       61 cgaaggcggt gcgccagaaa acgccgggtc gaaggtcgcg atcaagaaaa acccgctcgc
      121 cgcggtcgct tccggcagca tcggcaagcc ggagggcccg ccgccgatgc ccgagagcct
      181 gctcaagcaa ccagtgctct ataccgacaa gcccgggcag atcacggccc tggcttgcag
      241 cccctgggcg cccctggtgg cggttgcggg ccagaagcag gtcgcgatct ataactccga
      301 caacgcccag ctcgtgggca ttctgccgtt ccccgaaggc atcccgcatg tcgtccgctt
      361 cagccgcaac ggcgccgtgc ttctggttgc cggtggccgt ggagggcatt caggctgtgt
      421 ggtgctgttc gatgtgaaga cgggcaagcg gatggcaagg ctgggcgagg agaccgacgc
      481 catcctggcc gcggacatca acgccacgca cacgctggtt gctctgggcg gaccgagcaa
      541 ggtggtcagg atcttctccg tgcaaacggg cgagatggtc ggcgaaattc gcaagcacac
      601 ggactggatc tacggcgtcg agttcagccc ggacggggtg ttgctcgcca ccagcgaccg
      661 tagcggcgga ctgttcgtct gggaagcgga tacggctcgc gagtatctga atcttcgcgg
      721 acacaacggg gcggtctatt gcgtgagctg gcggatggat tccaacattc ttgccagcgg
      781 gggcgaagac accaccgtca agctgtggga aatgaacgac ggcaacgcga tcaagtcgtg
      841 gggcgcgcat ggcggcggcg cgttctgcgt cagctatgcg cacgacggac ggctggtgac
      901 ggccggccgc gacaatacgg ttaagagctg gaacggcgac ggcgcggcac tgaagacgtt
      961 ccccggattt cccgaagcgg ggatgcgctg tgcgttcacc tacgacggca agcgcgttgt
     1021 ggggggcgac tggcttggca acgtgaaggt ctgggatgcc gagcaggcag tcgaagccta
     1081 tacgctggct gccaatccgc cgacgctcgt gatgcgggtc gatgccgcga aggccgatct
     1141 ggccgccagg caagcggcgg cagccgccgc ggcaggggag tatgacgcgg cagtgaagct
     1201 ggcggctgag aaggacgcgg ccctgaaggc ggccggcgaa aagcatacgg ccgcggttgc
     1261 tgcggctcag aagacggaag ccgatcgcgt cgctgccgtg aaggcttcag aagttgccgc
     1321 cgctgccgtc aagccggcga tggatgcggc tgcggcggct caaaccgctg ctgtgacagc
     1381 cgggaatgag aaagcggccg cggataaggc tgccggagat aaagctgcgg cgacgaaggt
     1441 cgtcgccgat caggccgcgt cggaaagagc cgctgccgac aagctcgccg ctgaaaagag
     1501 cgccgaggcc ccggcggctg ccgagaaggc cgcagccact gcggttgtgg cagctcaagc
     1561 cgatgccgag cgggcgcagc tcgccgtcgt tgccgcccaa aagaccgaag cggccacgaa
     1621 gacgtccaac gcgctgacgg ctgccaacgc gaagctcgcc gaagcccaga acgcggccac
     1681 gacggcagcc aagcaggtgg aaactcaaac cgcggccgcc aaagccgctg cggacgccgt
     1741 tccggtcgcc aaggccgagg tcgataaggc cacgcaggaa aaggcggccg ccgatgccgc
     1801 cgcagcggcg aagaaacagg ccgccgacgc cgccgcggct gccgcggctg cgtcgcaagc
     1861 cgctctcgcc caggcggaag ccgataaggt cgccttcgag cagatggccg gcaagctctc
     1921 tgcggccctc gacgaagccg gcaagaaaat caccgctcag caagccgccc tcacgcaagc
     1981 cgaggccgag agcaagaccc tgggcgacgc tgtggctgcc aagaccgccg tcagcaagga
     2041 cctgaccgac aagatcgccg ccctgcaggc ggaactggcc aaggcccagg ccgaccagaa
     2101 ggccgccgaa gaagctgccg ccgccaaggc caaggccgcc gctgacgccg gtgccgctct
     2161 agccgaagcc caagccgccg cagaagcggc cgcggccgag cagaaagcgt ttgcggaagc
     2221 gtacgcggcg aagtagtggc gcaacaaaat gggcgtcccc ctccagggcg cccattttct
     2281 cgtttcaagc tgagtggcag gcaagatgcc tgccccactt acatcgcgtc gcgcgggcgg
     2341 atggcgccga ccaggccgtt gtagtcgtag cggtcgttct cgtggaacag cggcaagccg
     2401 agttccagcc gcttccgcag aatttcgatc ttctccggcg agccggcagg aaggtcggtg
     2461 gcctcgaact tctggtgcgg gtccggcgac cactcttcgt cgtggccata cttcagaatt
     2521 gcttcaaaca cattcttgca gcgttccatg ttctctccag tggaagatat gctcagccgg
     2581 gcaatggatg caaaagggcc gccacgctcg gcagaaggtt tcgtaagagg cgtcgttttc
     2641 gggctctcaa gcgggggaga gccggcagcg ggagatccgc aatgctcgtc gtctcaggtt
     2701 cgagcaatta tttttatttg accctgctgc gtcaagcttt ttgaagcgaa attttcgaag
     2761 tcgggaggga aattcatctt ccccaagcga aaatgtgccg ccttgggaag tcctgcggac
     2821 tgagcattgt cgctttttcg caaagcacat agttacaatg ctttacgatg aatttccaag
     2881 cagatcatcg atactcgttg atgtttgtga aatcaagaac gaagttttat cttccctaat
     2941 cacaatgcca atggcggccc tattgacagc cgttgctctg tcgctgaaag aacagaattg
     3001 agaccggcga gtctctctgg gcgaggaaac acgatgacct ggatccagcg acaaatgtcc
     3061 ctgcccgctc gcaaacgcgg cttccatttg gtgacccggg aggtcgtcga ggccctcccg
     3121 gaaattcgga tgtatcgcgt cggattgctg cacgtcttca ttcagcacac gtccgccgcg
     3181 ctcacgatca atgagaatgc cgatgcggac gtccggcacg atctggagag ctcgctgaat
     3241 gcgatcgcgc cggaggagtt tccgtacgtc cacacgatcg aaggcccgga cgacatgccc
     3301 gcgcacgtca aggcatcgct gatgggctcg tccgtttcaa tccccatcgc cgatggacag
     3361 cttttgctcg gcacctggca gggcatctac ctctgcgagc atcgcaacca cggcgggaat
     3421 cggaagctcg tgctgacttt gtggggtgaa tagtggggca ggcatcctgc ctgccgcgag
     3481 caccctcacc ccggccctct cccaagggga gagggagatc acgcggtcac ctggcaggct
     3541 ggaagcctgc cccacgacgt gccgcctggc tcaactcatc cagccaccac tcgatgaacg
     3601 tcgcctggtt cacgttgctg tcgatgtgag cgtaggcgtc gaggcacacg tcgaggcaag
     3661 cggcggcgga ttcctggccg gcgatccagc ggctggcgcg ggcgatggcg ctcgtcaatg
     3721 gcttgtcctg cgcgtcacac agggcagcat cgccgagaag cagcgagcga taaaactcct
     3781 cggcgagcga gacggccaat cgcagccggt cgcgcttggc ggccgattcc ttaccggctc
     3841 cttcgacaaa ttggccgacg agcttcgccg cctgaagcag ctccatctcg ggattggcga
     3901 gcgcgtcgag cagttggctg cggaattcga tcagctcggg ctcgcaccac tgggccgcgt
     3961 gggccacgga gcccccgctg cgcgccgccg cttgctgtgc aaccgcccga tcctcgcaca
     4021 agccgcattg cagaaggagt tcttcgacgt cgctctgcgt cagcggccta aacggcacaa
     4081 tctggcaccg cgagcgactc gtgggaagct
//
LOCUS       DASUGB010000914         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_657154, whole genome shotgun sequence.
ACCESSION   DASUGB010000914 DASUGB010000000
VERSION     DASUGB010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657154"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFV87_17360"
     CDS             complement(<1..194)
                     /locus_tag="VFV87_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105593.1"
                     /translation="MSMKVTCARCGARLVYKGTATLVRPCPKCQAPIAFIPTELQPAP
                     SGAVTPVAEAPITAVAVEET"
     gene            385..2706
                     /locus_tag="VFV87_17365"
     CDS             385..2706
                     /locus_tag="VFV87_17365"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HEX5105594.1"
                     /translation="MGRMPACFLVFASLAAFLPAASGADDGVIRSAKNGPWSAAETWA
                     GGKVPAAGARVHVLPGHTVTYDVQSDDVIRAINVAGTLRFARNRDTLLNTGLIKIEMN
                     EKFSEEGFDCDHFSEEPDKNMPRATLEIGTPNEPIPANHKAVIRLHHVEGLDPQSCPA
                     IVCCAGQMDFHGAPLSRTWVKLGATAKKDTAEVVLADEVTGWQVGDQVVITSTNWDDQ
                     VDDASEPRTIQAIEGTKITLDQPLKYDHLGEGDERGEIANLSRNVIVESAEPAGVRGH
                     TMYHRGSAGAISYAEFRHLGKKDTLGRYSLHFHLCGNSMRGSYVVGASIWDSHNRWLT
                     IHGTNYLVARDNVGYQSVGHGFFLEDGTEIYNLLDRNLAIAARRGKRLPKQVLPFDQN
                     EGAGFWWSNSLNTFTRNVAADCGEYGFRLEATPSSSFDIASLEVMQPDGSFRKTDIRT
                     LPFVRFEGNEVHSSSGLYGINLGEGVRGVGPDTKHPFIVRSTKIWNVHYGLRPQVPSL
                     LVENISIKSHYGVYHPNFDNHVYRHVTIRHSHTEPFNRGHDDDSRQDGVLAVDGLVFD
                     SCGNSGMPLIQISDDNSNGTAESHFRGVQTVNWSENNRRRQAALVNLGGGPRPQPKTA
                     KGVPVYLHDYYGEGRTALVVSTRSPEYKADPGKFREDAPLTGDESRVAEVSGVEFPEL
                     LAPVDDLPPQTVITHVIRQKDGKVLVRGTASDNGEIKRVVVNGQNAKLDVVTGQWETL
                     VTISDDDGRLSAYTEDIMGNVERMPHVVNVAGR"
     gene            complement(2724..>2903)
                     /locus_tag="VFV87_17370"
     CDS             complement(2724..>2903)
                     /locus_tag="VFV87_17370"
                     /inference="COORDINATES: protein motif:HMM:NF014761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105595.1"
                     /translation="VMEIIINGKDLAAIIRATQAAIAASVNTPGLLRISAGNYNGRLG
                     KSFIYLHPSKQPPAE"
BASE COUNT          581 a          919 c          904 g          499 t
ORIGIN      
        1 tgggtctctt caactgcaac cgcggtgatg ggcgcttctg cgacgggcgt aacggcccca
       61 gaaggcgccg gctgaagctc ggtcggaata aaagcgatcg gcgcctggca tttcgggcag
      121 ggccgaacca gcgtggcggt ccctttgtag accagccttg cgccgcatct ggcgcaagtc
      181 actttcatcg acatcgtcac ctcgccgata gcgctttcaa aggctcattc agactatgac
      241 tgcttccgcg cgcctgtcaa cccaaaagga actcgggcgc ctgcgtccgg catccagcag
      301 gccgcgaaac atttgctgcc cgcgtggccc ttggtgtatg ctgactacac tgcctgccca
      361 ctttcccacc ttggagaccc cgccatgggt cgaatgcccg cctgtttcct tgtattcgcg
      421 tcgctcgccg cgttcttgcc cgctgccagc ggcgcggacg acggcgtcat ccgctcggcc
      481 aagaacgggc cgtggtcggc cgcggaaacg tgggccggcg gcaaagtgcc cgcggcaggc
      541 gccagagtgc acgtgctgcc agggcatact gtcacgtacg acgtccaatc tgatgacgtc
      601 atccgggcga tcaacgtcgc cggcacgctg cggttcgccc ggaatcgcga cacgctcctg
      661 aacacggggc tcatcaagat cgagatgaac gagaaattca gcgaggaggg cttcgattgc
      721 gatcacttct ccgaggagcc ggataaaaac atgccgcggg caacgctcga gatcggcacg
      781 ccgaacgagc cgattccggc caaccacaag gcagtgatcc gcctgcacca cgttgaaggg
      841 ctcgatccgc agtcgtgccc ggcgatcgtc tgctgtgcgg gacagatgga cttccacggc
      901 gcgcccttga gccgcacgtg ggtcaagctg ggagcgacgg cgaagaaaga caccgccgag
      961 gtcgtgctgg ccgacgaagt gaccggctgg caggtgggcg atcaggttgt gattacctcc
     1021 accaattggg acgaccaggt ggatgacgcc tccgagccgc ggacgattca agcgatcgaa
     1081 ggcacgaaga tcacgctcga ccagccgctg aagtacgacc atctcggcga aggggacgaa
     1141 cggggcgaaa tcgccaacct gtcgcggaac gtcatcgtcg aatcggctga accggcgggc
     1201 gttcgcgggc acacgatgta tcaccgcggc tcggccggcg cgatcagcta cgccgaattc
     1261 cgccacttgg gcaagaagga cacgctcggc cgctacagcc tgcacttcca tctgtgcggc
     1321 aactcgatgc gggggagcta cgtcgtcggc gcctcgatct gggacagcca caaccgctgg
     1381 ctcacgatcc acggcaccaa ttacctcgtg gcccgcgata acgttggcta ccagagcgtc
     1441 ggccacggtt tcttcctgga agacgggacg gagatctaca acctcctcga ccgcaacctc
     1501 gccatcgccg cccgccgcgg caaacggctg cccaagcagg tgctgccgtt cgatcaaaat
     1561 gaaggcgccg gcttctggtg gtccaacagc ctgaacacgt ttacgcggaa cgtcgcggcc
     1621 gactgcggcg aatacgggtt ccggctggaa gcgaccccct catcgtcgtt cgacatcgcc
     1681 tcgctcgaag tgatgcagcc ggatggcagc ttccgcaaga ccgacatccg cacgctgccg
     1741 ttcgtgcgct tcgaagggaa tgaggtccac agcagctcgg gcctgtatgg catcaatctg
     1801 ggcgagggcg tgcggggcgt tggtcccgac acgaagcacc cgttcatcgt ccgcagcacg
     1861 aagatctgga acgtgcacta cggtcttcgc ccgcaggtgc cgtccctgct ggtcgagaac
     1921 atctcgatca agtcgcacta cggcgtgtat cacccgaatt tcgacaacca tgtctatcgc
     1981 cacgtcacga tccgccacag tcacacggaa ccgttcaacc gcggccacga tgatgacagt
     2041 cggcaagacg gcgtactggc cgtcgatggt ctggtgttcg atagctgcgg gaacagcggc
     2101 atgccgctga tccagatctc ggacgacaac agcaacggca cagcggaatc gcatttccgc
     2161 ggcgtgcaaa cggtgaactg gagcgagaac aatcgccgcc ggcaggcggc gctggtgaac
     2221 cttggcggcg gcccccgccc gcagccgaaa acggcgaaag gcgttccggt ctatttgcac
     2281 gattactacg gcgagggccg gaccgccctg gtcgtcagca cgcgcagccc cgaatacaaa
     2341 gccgatcccg gcaagttccg cgaagatgcc cccctgaccg gcgatgaaag ccgcgtggcc
     2401 gaagtcagcg gcgtcgaatt ccccgagctg ctcgcacccg tggacgatct cccgccgcaa
     2461 accgtgatca cccatgtcat tcggcagaag gacggtaagg ttttggtgcg cggcacggcc
     2521 agtgataacg gcgaaatcaa acgggttgtc gtcaacggcc agaatgcgaa gctcgacgtt
     2581 gtcaccggcc agtgggaaac gcttgtgacg atttccgatg acgacggtcg tctctcggca
     2641 tacaccgaag acattatggg taacgtggaa cggatgccgc acgtggtcaa tgtcgccggc
     2701 agataacatg ccgaggatac ttttcactct gccggcggtt gcttgcttgg atgcaagtag
     2761 ataaagctct tgccgaggcg gccgttgtag ttgccggcgg agatacgcag caggcccggc
     2821 gtgtttacgc tggcggcaat agcggcttgc gtggcacgga tgatggcggc caggtccttg
     2881 ccgttgatga tgatctccat cac
//
LOCUS       DASUGB010000915         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1213686, whole genome shotgun sequence.
ACCESSION   DASUGB010000915 DASUGB010000000
VERSION     DASUGB010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213686"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFV87_17375"
     CDS             <1..612
                     /locus_tag="VFV87_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5105596.1"
                     /translation="EEGRLTDSFGRRVDFRNAILIMTTNAGAEAIKNESQFGFAKADA
                     DSSYEGMKERVQERIERAFRPEFINRLDDIIVFRHLTTEDLKQVIDLELKKVRERLLE
                     RGFTLQLTDSAKEFIIKKGSNTDFGARPLRRAVENYIEDPLAEEILKGEFQGKDTIVV
                     DAIKDDEDKPRRLDFKGQVTGNQPPVQPEPVPAGAAAGEAEGK"
     gene            802..1701
                     /locus_tag="VFV87_17380"
     CDS             802..1701
                     /locus_tag="VFV87_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5105597.1"
                     /translation="MPRPAALPYIWMLLGAASFSVMAALTAGLRDQCDWQWIAIARTG
                     LAMIFAASLAVGGGARLVFFRPARLWMRSLAGSISLVCGFYAMTHYPVSEVLTLTNMF
                     PLWVAVLSWPLLGQSPPLDIWPAMLVSLVGIVLMQQPDFQGHSTYLPIGAAVVSSLTS
                     AVAMIGLHQIKQIDPRAIVAHFSAVALAASVAALFVFPTSRPLALDLKTGGLLLGVGV
                     SATIGQLFLTKAFAAGPPARVSVVALAQVGFTMLLEIAFWHRPFGMLTLVGIALVVAP
                     TAWTLLRSAGRPRLAIHDGERVA"
     gene            complement(1698..>3079)
                     /locus_tag="VFV87_17385"
     CDS             complement(1698..>3079)
                     /locus_tag="VFV87_17385"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5105598.1"
                     /translation="FLRRVSLDLTGAIPRVADVRAFLADQAPDKRARMIDRLLSSPAH
                     ATHLANTFRHIMLPGGMDLEQINNIVGVQNWLRQRFVENLRYDNLVGDLLTASSGDDV
                     GPALYYTSLELAPEKLAASTARIFLGLQIECAQCHDHPHDHWKQKDFWGYAAFFARLR
                     RPDENQPRMQVALVDLDEGEARLPDSGEVVPPRYPSGGAPGKDELGTRREQLAIWMAS
                     RDNPYLPRAAANRGWSILFGRGIVEPVDDLGPQNPPSHPELLDELTQYFIDSGFDFRE
                     LLRTLAGTEAYQRTSEWSGEKQPPPELYACMAVKTLTADQLYDSVNRVLNRRGQGMMP
                     GANVQSPLLDPQRQAFIAKMEIKGLPMLEYQAGVLQALTLLNGADLTEASDPQRSPLI
                     GALEFPLLSDDERIQTLFLATLSRPANDDEVKLVQAQLASHSQSDKAKVWSDMLWALL
                     NSAEFALNH"
BASE COUNT          486 a         1028 c          986 g          579 t
ORIGIN      
        1 gaagaaggcc ggctgaccga cagcttcggc cgccgggtcg atttccgcaa cgcgatcctg
       61 atcatgacca ccaacgcggg ggccgaggcg atcaagaacg aatcgcagtt cggctttgcc
      121 aaggcggacg cggattcgtc ctacgaaggg atgaaggagc gcgtgcagga gcggatcgag
      181 cgggcgttcc ggccggagtt catcaaccgc ctggacgaca tcatcgtctt ccgccacctc
      241 accaccgagg acctgaagca ggtgatcgac ctggagctga agaaagtgcg cgagcggctg
      301 ctcgagcgcg gcttcacgct gcagctcacc gacagcgcca aggagttcat catcaagaag
      361 ggatcgaaca ccgatttcgg cgctcgcccg ctccgccggg ccgtcgagaa ctacatcgag
      421 gacccgctgg ccgaggaaat tctcaagggc gagttccaag gcaaggatac gatcgtcgtc
      481 gatgccatca aggacgacga agacaagccc cgccggttgg acttcaaagg ccaggtgacg
      541 ggcaaccagc cgcctgtcca acctgagccg gtccccgccg gggcggctgc cggcgaagcg
      601 gaagggaaat agcttgccac gccttttgta aatgcgtcct gaccccgggc ggcttgtccg
      661 gggtcttttt ttgctgtagc gaaagagcgc gaacctcgtt tctcaggcgc tctctttgca
      721 atcttcatta gctccgcctg ggcgcttaca atgggcactc ccgcccccaa atccgcgctt
      781 cgagttccgc gcttctgccg tatgccccgc cccgccgccc tgccctacat ctggatgctc
      841 ctgggagccg cctcgttctc cgtcatggcg gcgctgacgg caggcctgcg ggaccagtgc
      901 gattggcagt ggattgcgat cgcccgcacc ggcctggcca tgatcttcgc cgcgtccctg
      961 gcggtcggcg gcggcgcgcg gctggtcttt tttcgtcccg cccggctctg gatgcggagc
     1021 ctcgcgggca gcatcagcct ggtctgcggc ttttatgcca tgacgcacta cccggtgtcg
     1081 gaagtgctga cgctgaccaa catgttcccg ctgtgggtcg cggtgctttc gtggccgctc
     1141 ttggggcagt cgccgccgct ggacatctgg cccgccatgc tggtcagcct ggtgggcatc
     1201 gtgctcatgc agcagcccga ttttcaaggg cacagcacct acctgccgat cggagcagcg
     1261 gttgtcagct cgctgacgag cgccgtcgcc atgatcggcc tgcaccagat caagcagatc
     1321 gacccgcggg cgatcgtggc ccatttttcc gccgtcgcgc tggccgccag cgtcgctgcc
     1381 ctcttcgtgt tcccaaccag cagaccgctg gccctggacc tcaagaccgg cgggctgctg
     1441 ctgggggtcg gtgtgtcggc gacgatcggc cagctattcc tcaccaaggc cttcgccgcc
     1501 gggccgcccg ctcgcgtttc ggtggtggcc ctggcccagg tcggcttcac gatgctcctg
     1561 gaaatcgcgt tctggcaccg ccctttcggc atgctgacgc tggtgggcat cgccctggtg
     1621 gtcgccccca cggcctggac gctccttcgc agcgcaggcc gcccgcgact agcgatccac
     1681 gatggtgaac gggtcgctta gtgattcagc gcgaattcgg cgctgttcaa tagggcccac
     1741 agcatatcgc tccagacttt cgccttgtcg ctttggctgt ggctggcgag ttgggcctgt
     1801 accagcttca cttcatcgtc gttcgccggg cgggagagcg tcgccagaaa gagcgtttgg
     1861 atccgctcgt cgtcgcttag cagcggaaat tcgagggccc cgatcagcgg gctgcgctgc
     1921 gggtcgcttg cctcggtcag gtccgcccca ttgaggagcg tgagggcttg gagcacgcct
     1981 gcctggtatt ccagcatcgg cagccccttg atttccatct tggcgatgaa cgcctgacgc
     2041 tgcggatcca ggagcgggct ttgcacgttg gcccccggca tcatcccctg cccgcggcga
     2101 ttgagcacgc ggttcacgct gtcgtaaagc tgatcggcgg tcagcgtttt taccgccatg
     2161 caggcataca gttccggggg cggctgcttc tcgccgctcc actcgctggt ccgctggtag
     2221 gcctcggtac cggccagtgt tcgcagcagt tcgcggaaat cgaacccgct gtcgatgaaa
     2281 tactgcgtca gctcgtcgag caattccggg tggctcggcg ggttctgcgg ccccagatca
     2341 tcgaccggct cgacgatgcc gcggccgaac aggatggacc agccgcgatt ggcggccgcc
     2401 cgcggcaggt acgggttgtc gcgggaggcc atccagatcg ccagttgctc gcgacgggtg
     2461 cccagctcgt ccttgcccgg cgctccgccc gaaggatacc gcggcggcac cacctcgccc
     2521 gaatccggca gccgggcctc cccctcgtcg agatcgacga gggccacctg cattcgcggc
     2581 tggttctcgt cgggccgccg gaggcgggcg aagaatgcgg cgtagcccca aaaatccttc
     2641 tgcttccaat ggtcgtgcgg gtgatcgtgg cactgggcgc attcgatttg cagccccagg
     2701 aaaatccgcg ccgtgctcgc cgccagcttc tccggcgcca gctccagcga cgtgtaatac
     2761 agtgccgggc cgacgtcgtc tccagagctg gccgtcagca ggtcgcccac caggttgtcg
     2821 taccgcaggt tttcgacgaa ccgttgccgc agccagttct gcacgccgac aatgttattg
     2881 atctgctcca ggtccatgcc gccggggagc atgatgtggc ggaacgtatt ggccagatgc
     2941 gtcgcatgcg ccggcgacga gagcagccga tcgatcatcc gggcccgttt gtcgggcgct
     3001 tggtcggcca gaaaggcccg cacgtcagcc acgcgcggga tcgccccggt caaatcgaga
     3061 ctcacccgcc gcaagaatt
//
LOCUS       DASUGB010000916         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1632184, whole genome shotgun sequence.
ACCESSION   DASUGB010000916 DASUGB010000000
VERSION     DASUGB010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632184"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="VFV87_17390"
     CDS             complement(<1..1863)
                     /locus_tag="VFV87_17390"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5105599.1"
                     /translation="MDRRVLLGCAVLLAAMVGCAKEGKPPDTAGSPATHADARSPGEG
                     GGAAQTHRPVIVGPETKSTTIEFPDDVVEGDLIRPPVDFQPAPETAGPLSAEPPVVAP
                     RSLSNPLRTSDVPREAPNPLRSFSTLPQMAEPREAAAPQFAPSAAPFAPMSIEPSAGT
                     AAGGSDAEAAQRAAEAALAEARRAMEAEAAAKAAAEEGALRARATELAPSRETTQPTE
                     PAQPFDVVQVFYGTDRAAIEPQAGGIAAHVMRFLPTAFCALVTLCLVLIASSRKSVAL
                     WSLAIVGTAACIGLGYQATAGTLFAIRWEGKLGPQYTVDRAPGGEVQLGVCEISIPKI
                     HKPGELEAPSILRLEVREDAAQHVVLTKTERLADEKFYALLRERVQASPRRELFVFIH
                     GFDTQFEDAARRTAQIHHDLKFQGAPIFFSWPANNKSVLTYPADENTIGWSAPHFKQF
                     LLSIVKESGAQSINLIAHSMGNRALSVALREIDLEFRGQSRLFNQVVLAAPDIDADDF
                     RTNIAPAMQHTANHVTLYASSRDDALRVSQLLHRGARAGDSGRGLVVLPGMDTIDVTA
                     IDTSPWGHIYLGASDPVLQDLKHLFLYGFPPQERRWLSPAQREGLTYWIFEATQT"
     gene            2258..>2846
                     /locus_tag="VFV87_17395"
     CDS             2258..>2846
                     /locus_tag="VFV87_17395"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105600.1"
                     /translation="MHDYELNRRQALLVGGLGALSLGMPGAVIGSDKVDAYGNATRSE
                     KSCIFILLCGGPSHIDTWDMKPDAPASYRGPYKPIATKVPGMRINEMHTELAKLTDEF
                     LLINSMTHPGAISNHFEAMHNLLSGQSAKRVQEGMPDDQPYLGSFVAKHKPSKTNLVS
                     NAWLIKCVGPPVFCAPNIGIGGYLGSAYAPVFVGSA"
BASE COUNT          493 a          931 c          927 g          495 t
ORIGIN      
        1 cgtttgggtg gcttcgaaga tccagtaggt cagcccttcg cgctgcgcgg gggagagcca
       61 gcggcgctct tgcggcggga agccgtagag gaacaggtgc ttgaggtctt gcagcacggg
      121 gtcgctggcg ccgaggtaga tgtggcccca ggggctggtg tcgatcgccg tgacgtcgat
      181 cgtgtccatg ccgggcagca ccaccaggcc gcggccgctg tcgccggccc gggcgccgcg
      241 gtgcaaaagc tgcgaaacgc gcagggcgtc gtcgcggctg gaggcataca gcgtcacgtg
      301 attggccgtg tgctgcatgg cgggggcgat gttcgtgcgg aaatcgtcgg catcgatatc
      361 cggcgcagcc agcaccactt gattgaacag acgcgactgg ccgcggaact ccagatcgat
      421 ttcgcggagc gcgaccgaga gggcccggtt gcccatgctg tgggcgatca ggttgatcga
      481 ctgggcgccg ctctccttga cgatcgacag caggaactgc ttgaagtggg gcgcgctcca
      541 accgatcgtg ttctcgtcgg ccggataggt gaggaccgac ttattgttgg cgggccaact
      601 gaagaagatc ggcgccccct ggaacttcag gtcgtgatga atctgggccg tgcggcgggc
      661 agcgtcttcg aactgtgtat cgaacccgtg aatgaagacg aatagctccc ggcggggcga
      721 ggcttgcacg cgctcgcgca aaagggcgta gaacttctcg tccgccagcc gctccgtttt
      781 cgtcaacacc acgtgctgcg cggcgtcttc gcggacttcc aggcggagga tcgacggcgc
      841 ttccaattcg ccgggcttgt gaatcttggg aatcgaaatt tcgcagacgc cgagctgcac
      901 ttcgccgccc ggcgcgcgat cgaccgtgta ttgcgggccg agcttgcctt cccagcggat
      961 tgcgaacagc gtgccagctg tcgcttgata gcccaagccg atgcacgccg ccgtgccgac
     1021 gatggccagc gaccagagcg cgacactctt gcggctggag gcgatcaaga cgaggcagag
     1081 tgtgacgagg gcacaaaaag ccgtagggag gaagcgcatg acatgcgccg cgatgccgcc
     1141 cgcctgcggc tcgatcgcgg cccggtcggt gccgtagaag acctggacga cgtcgaaggg
     1201 ctgagccggt tcggtgggct gagttgtttc tcgggacggg gcgagttcgg tcgcccgggc
     1261 acgaagcgcg ccttcctcgg cggcggcttt ggccgcagct tctgcttcca tcgcccggcg
     1321 ggcctccgcg agagccgcct cggctgcgcg ttgcgccgct tccgcgtcgg agcctccggc
     1381 tgctgtgccg gcggaaggct cgatcgacat gggcgcaaac ggagcggctg atggagcgaa
     1441 ctggggcgcg gcagcttctc gaggttcggc catctgcggc aaggtggaga acgaccgcag
     1501 cgggttgggc gcttcgcgcg gcacgtccga cgtccgcagc ggattcgaca agctgcgcgg
     1561 cgcgacaacc ggcggctcag cggacaaggg gccggccgtt tccggtgcgg gctgaaaatc
     1621 gacgggcgga cgaatcaggt cgccttccac cacgtcgtct ggaaactcga tcgttgtgct
     1681 cttcgtttct ggcccgacga tcaccggacg atgcgtctgg gccgccccgc ccccctcgcc
     1741 gggcgatcgt gcgtcggcgt gggtcgccgg cgatcccgcg gtatctgggg gcttcccctc
     1801 cttcgcacag cccaccatcg ccgcgagcaa tacggcacaa ccaagcagca ctcgccgatc
     1861 catggcagca ctcccgacca agaagtcgat catcaggcag gcgcacagca gcacaccttc
     1921 cggggccagg atgatagcgg ccgattgcgt cgcgggccag agcgaagcgt cgctttgcct
     1981 tggaatcgtg ccgcaagtcg ctacccactc cgatcgcttc ttcccaggca tccgccgtgc
     2041 aaacatcgct gctagctaac ccgcccctcg ccacgcatct tcttggctct tggctcagca
     2101 tcggattcta cggcaacgac tgcacaacaa gggtcctcgt ctcttctttg acaaatccag
     2161 ctcaaccgtg cagaattggc acatgccttc ggccctccga gcagcgccgc cggctcccgc
     2221 ctgtttcctc gccctaccct ccaaccccag ccctcccatg cacgattacg aactcaatcg
     2281 ccggcaagcc ctcctcgtcg gtggactcgg cgcactcagc ctcggcatgc ccggagccgt
     2341 catcggcagc gacaaggttg atgcatacgg gaatgcaacg cgttcggaaa agtcgtgcat
     2401 tttcatcctg ctctgcggag gcccgagcca catcgacacc tgggacatga agcccgacgc
     2461 gccggcgtcg taccgcggtc cgtacaagcc gattgccacc aaggtgccgg gcatgcggat
     2521 caacgaaatg cacaccgagc tcgcgaagct caccgacgag ttcctgctga tcaattcgat
     2581 gacccatccg ggggcaatca gcaaccactt cgaagcgatg cacaacctgc tcagcggcca
     2641 atcggccaag cgcgtgcagg aaggcatgcc cgacgaccag ccgtacctcg gctcgttcgt
     2701 cgccaagcac aagccgagca agacgaatct cgtttccaac gcgtggctga tcaagtgcgt
     2761 tggcccgccc gtcttttgtg ccccgaatat cggcatcggc gggtatctgg gctcggccta
     2821 cgccccggtg tttgtcggct cggctg
//
LOCUS       DASUGB010000917         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1908233, whole genome shotgun sequence.
ACCESSION   DASUGB010000917 DASUGB010000000
VERSION     DASUGB010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908233"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="VFV87_17400"
     CDS             complement(<1..1956)
                     /locus_tag="VFV87_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX5105601.1"
                     /translation="MTPEITTQRQRELIGRLTRLVARRAKDEVEIRRLYAERDARLER
                     EYSRDRQQRATDFERQHETLVREFQDTREAVYRQYESAGCELAEEEHQKLEALSERNS
                     ESLEDAKHLWQHRKDDVESTYTMRQGQPQRDFNQFQQQCEYRETELQGLIDQAQKIVR
                     RRCGWPSEEAPPPLPPAGLTKAQHVERINAAIARAKAKIHELQHAPAAAFLDSGWPAL
                     IFFLSACVTGPVAWFFLSHVEWYWIVSAAAALSLLLSLGSYYVALPLARRQMLRILPE
                     FQLAVEQARGDLAAVLAIAKAESEKKSRKLLRRREKGLKAAQAEWEQVGQQQNEQHQA
                     EVRKVSGEIEARRQRVTQPFERQLDEIAVKYPPLITRLERDFEDQAAALHQRYGEART
                     ASREQYQREWTDLVTSWTAGLAEFQEVIGAMNDYCDSRFPDWDEIAGDSSQLGTRSAN
                     SSPRNGDSAVSVPALRFGRFDLELGSLEGGLPQDAELELPPARFQLPAVLSYPECPSL
                     LIEAEGDGRDAATRLIQNVMLRLLTTFPPGKVRFTIIDPVGLGQNFSAFMHLADYDQR
                     LVTSRIWTETPHINQRLADLTEHMENVIQKYLRNEFASIQEYNQQAGEVAEPFQILVV
                     ANFPANFSDESARRLVSIASSGARCGVY"
     gene            complement(2011..2250)
                     /locus_tag="VFV87_17405"
     CDS             complement(2011..2250)
                     /locus_tag="VFV87_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105602.1"
                     /translation="MRICDLNTGLGQLAQAMANLKARWAEAKTHWHDESSRQFEKTHL
                     SELPARMQHVVQAAQRLSAALEAAERELSDRPDEG"
     gene            complement(2247..>2583)
                     /locus_tag="VFV87_17410"
     CDS             complement(2247..>2583)
                     /locus_tag="VFV87_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105603.1"
                     /translation="LNEARLALQRCELTIDEDHRRSCYDERKALERAKRRLHQCEDKV
                     QAVRRWLPQIRKEVEEFEVQVARLKQFLESDFPQAAAALERMAAALDQYVQQQAAAAD
                     GIAGAKEPS"
BASE COUNT          412 a          872 c          791 g          508 t
ORIGIN      
        1 ataaactccg catcgcgcgc cgctggaagc gatgctcacc agccggcggg ccgattcgtc
       61 ggagaaattc gccggaaaat tcgcgacgac gagaatttgg aacggctcgg cgacctcgcc
      121 ggcctgctgg ttatactcct ggatcgaggc gaactcgttg cggaggtatt tctggatcac
      181 gttctccatg tgctcggtca ggtcggccag ccgctggttg atgtgcggcg tttcggtcca
      241 gatgcgactt gtaaccagcc gctggtcgta atcggccagg tgcatgaagg ccgagaagtt
      301 ctgaccgagg cccaccgggt cgatgatcgt gaagcggacc ttgcccggcg gaaacgtcgt
      361 aagcagccgc agcatgacgt tctgaatcag gcgcgtggcg gcgtcgcggc catccccttc
      421 ggcttcgatc agcagcgacg ggcactcggg atacgagagc accgcgggca actgaaagcg
      481 ggccggcggc aattccaatt cggcatcctg cggcaggccg ccctccaggc tgcccagttc
      541 caaatcgaag cggccgaaac gaagggcggg aacgctcacg gcagaatcgc cgttgcgcgg
      601 agagctgttt gcggatcgtg tcccgagctg cgacgaatca cccgcaattt catcccagtc
      661 gggaaaccgc gaatcacagt aatcgttcat tgcgccgatg acttcttgaa attcagccag
      721 gccagcggtc cagcttgtta ccaagtcggt ccattcgcgc tggtactgct cgcggctggc
      781 ggtccgggct tcgccgtatc gctgatgcag tgcggcggcc tggtcttcaa agtcgcgctc
      841 taagcgcgta atcagcggcg gatacttgac ggcgatctcg tcgagttgcc gctcgaacgg
      901 ctgcgtgacc ctttgccggc gggcctcgat ttcccctgac actttgcgaa cttccgcctg
      961 atgctgctcg ttttgctgct ggccaacttg ctcccactcg gcctgggccg ccttcagccc
     1021 cttttcgcgg cggcggagca gctttcgcga cttcttttcc gactcggcct tggcaatcgc
     1081 cagcaccgcc gccagatcgc cgcgggcctg ctcgaccgcg agttgaaact ccggcagaat
     1141 ccgcagcatc tgccgccggg ccagcggcag ggcgacgtaa taggaaccca acgaaagcaa
     1201 cagcgaaaga gcggctgcgg ccgacacgat ccaataccat tcgacgtggc tcaaaaagaa
     1261 ccaggcgaca ggacccgtga cgcaggcaga cagaaagaaa attagcgccg gccagcccga
     1321 atccaggaaa gccgccgccg gcgcgtgctg cagctcgtga atcttggcct tggcccgggc
     1381 gatggccgcg ttgatccgct cgacgtgctg cgctttggtc aagcccgccg gcggcaaggg
     1441 aggcggcgct tcctcgctcg gccagccgca gcgtcggcgg acaatcttct gtgcctggtc
     1501 gatcagcccc tgcagctccg tctcgcgata ttcgcactgc tgctggaact ggttgaagtc
     1561 tcgctgcggc tgcccctgcc gcatggtgta cgtgctctcg acgtcgtcct tgcggtgctg
     1621 ccagaggtgt ttggcatcct cgagcgattc gctgtttcgc tcgctcaggg cttcgagctt
     1681 ttgatgctct tcttcggcca gctcgcaccc ggccgattcg tactggcgat agaccgcttc
     1741 gcgcgtgtct tgaaactcgc ggaccagcgt ctcatgctgc cgctcgaaat cggtggcccg
     1801 ttgctggcgg tcgcggctgt actcgcgctc caggcgggcg tcgcgctcgg catagaggcg
     1861 gcgaatctcg acctcgtcct tggcccgccg ggcgaccagg cgcgtaaggc ggccaatcag
     1921 ctcgcgctgc cgctgggtcg tgatctctgg cgtcatcagt tcctgcgtcc catttgcccc
     1981 ctccattctt gcattctaca gccggcacgc ttacccctca tccgggcggt cgctcaattc
     2041 ccgctcggcc gcctccagcg ccgcggatag ccgctgggcc gcctgcacga cgtgctgcat
     2101 cctcgccggc agctccgaca agtgcgtctt ctcaaattgc cggctgcttt cgtcgtgcca
     2161 gtgcgtcttc gcctcggccc agcgagcctt caggttcgcc atcgcctgag caagctgccc
     2221 caggcccgtg ttcagatcgc agattctcat gagggctcct tcgcgccggc aatgccgtcg
     2281 gctgcggccg cctgctgctg gacgtattga tccagcgccg cggccatccg ctccagtgcc
     2341 gccgcggcct gcgggaaatc gctctccaga aactgcttga gcctggcgac ctgcacctca
     2401 aactcctcga cctccttgcg aatctgcggc agccagcggc ggacggcctg caccttgtcc
     2461 tcgcactggt gcagccgccg cttggcccgc tccagggcct tccgctcgtc gtagcacgag
     2521 cggcgatggt cctcgtcgat cgtcagctca cagcgctgca atgccagccg ggcttcgttc
     2581 agc
//
LOCUS       DASUGB010000918         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_380468, whole genome shotgun sequence.
ACCESSION   DASUGB010000918 DASUGB010000000
VERSION     DASUGB010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380468"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1263
                     /gene="fba"
                     /locus_tag="VFV87_17415"
     CDS             247..1263
                     /gene="fba"
                     /locus_tag="VFV87_17415"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650067.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX5105604.1"
                     /translation="MPLVPLRKVLDHAAANDYGVAAFNVNNMEQIQAIMEAAKETDSP
                     VIVQASRGARSYSQDNYLRHLMLAAAELYPQIPVVMHQDHGNSPATCMSAIDNGFTSV
                     MMDGSLKEDGKTPADYDYNVRVTTEVVKAAHARGVSVEGELGCLGSLEHGEGEQEDGH
                     GAVGKLSREQLLTDPDQAEDFVKKTNVDALAVAIGTSHGAYKFTKKPTGEILAMDRIV
                     AIHKKLPNCHLVMHGSSSVPQEIQDELNRYGGKMKQTWGVPVEEIQRGIKHGVRKINV
                     DTDNRMAMTAAIRRVLMETPEKFDPRDYLKPARDAMKKVCIARMVSFGQAGNASKMRS
                     AGLI"
     gene            1388..1531
                     /locus_tag="VFV87_17420"
     CDS             1388..1531
                     /locus_tag="VFV87_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105605.1"
                     /translation="MPQGKEVFLGLNQTGVIVFIVAGLICFPIGFIVPFFVDSCKSDP
                     NVK"
     gene            1666..2751
                     /locus_tag="VFV87_17425"
     CDS             1666..2751
                     /locus_tag="VFV87_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5105606.1"
                     /translation="MAALPVPALEARSPFEQLRLARQIVRAEGQTLLALAERLGDEFC
                     HAIGVLSAAQGSVIVSGMGKAGLIGQKIAATLASTGTRSHFVHPAEAIHGDLGRIHAD
                     DVALVLSFSGNTEEIVRILPALTQLGVPVVAVTGQPDSPLGRAAAVTLDLGPIREACP
                     HGLAPTASTTAMLALGDALALVLSRMRGFTPDDFARFHPGGNLGRKLARVEDAMRPLG
                     QCRVAKQTQTLRETLVHQSRPGRRTGAIMIVDDAGKLAGIFTDSDLARLLEANRDAAI
                     DGPLSAVMTQSPSTVLAGQPLSHACDLLAQRKISELPVVDSVGQPVGLIDITDVLGAE
                     PEAGEVQSPKSKVQSRTGAFKLLTPDS"
     gene            2776..3297
                     /locus_tag="VFV87_17430"
     CDS             2776..3297
                     /locus_tag="VFV87_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5105607.1"
                     /translation="MSLADLCRPIELILSDVDGVLTDGGIWYDNQGVELKGFHIRDGL
                     GIKVWQRAGFKFGVLTARTSHIVKLRAGELGIDLVRQGFEDKLPVAQEILRERGLSPE
                     QVCYIGDELTDLPVIRFVGLGVAVADAAEEVRAAAAHTTRTSGGHGAVRELVETILKA
                     KGRWDDVIQRYLA"
     gene            3376..>3972
                     /locus_tag="VFV87_17435"
     CDS             3376..>3972
                     /locus_tag="VFV87_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105608.1"
                     /translation="MLHFRRYIAALAVLLAAFGLYYVLVAPWLEPPPIGRPPVADIGP
                     VQPSPGVESELAELFPPDAWERDPQTKVVETSQCILLVKDYLPTPDGRLELNPCTLVF
                     YTEGDTASLAGGTAMAGGGTNRRPIVLQAPEGAVLIFDRPLEIGRGQFGQIVGGKLEG
                     EVTISSPPSQPDGDDALRLVTRNVQLAKNRIFTPGDVAI"
BASE COUNT          741 a         1338 c         1238 g          655 t
ORIGIN      
        1 atgccccagc tcatgcacca cctgcaacag cagccaggcc aggagtgcga cggtaacgat
       61 cagcacgact gccgaaagtc gcgggacacg ccagcctgct cgtggaagat cattcacctt
      121 agaagccacc tttgtggagc gcgccttgcc gctatcaagc gtacccagcc ggtcattgcg
      181 gctgtggcgg ggccagacgt atcattcaca acatcaggcg tttccgcgag cccgctggag
      241 tctcccatgc cccttgttcc tctccgcaaa gtcctcgatc acgctgccgc caacgactac
      301 ggcgtggcgg cgttcaacgt caacaacatg gagcagatcc aggcgatcat ggaggcggcc
      361 aaagagaccg attcgccagt gatcgtccag gcctcgcgcg gggcccggtc gtattcgcag
      421 gacaattacc tgcggcatct gatgctcgca gcggcggagc tctatccgca gattccggtc
      481 gtcatgcacc aggaccacgg caacagcccg gccacctgca tgtcggccat cgacaacggc
      541 ttcacgagcg tgatgatgga cggctcgctc aaggaggacg gcaaaacgcc ggccgactat
      601 gactacaacg tccgggtcac gacggaagtc gtcaaagccg cccatgcccg cggcgtctcc
      661 gtcgaagggg agctgggctg cctgggctcg ctggaacacg gcgaagggga gcaggaagac
      721 ggccacggcg cggtcggcaa gctctcgcgc gaacagctcc tgaccgatcc cgaccaggcc
      781 gaggatttcg tgaagaagac gaacgtcgac gccctggcgg tcgccatcgg caccagccac
      841 ggggcctaca aattcacgaa aaagccaacc ggcgagatcc tggcgatgga tcgcatcgtg
      901 gcgatccaca agaagcttcc caattgccac ctcgtgatgc acggcagctc cagcgtgccg
      961 caggaaatcc aggacgagct gaatcgctac ggcggcaaaa tgaagcagac ctggggcgtg
     1021 ccagtcgagg aaatccagcg cgggatcaag cacggcgtgc ggaagatcaa cgtcgatacc
     1081 gacaaccgca tggccatgac cgccgccatc cgccgggtgc tgatggaaac cccggaaaag
     1141 ttcgatccgc gcgattacct caagcccgcc cgggacgcga tgaaaaaggt ctgcatcgcc
     1201 cggatggtct cgttcggcca ggccggcaac gccagcaaaa tgcggtcggc ggggctgatt
     1261 taatccaaga ttctcatgca gttgacgaaa ttacacacgt cgccggcagt tgcacgcacc
     1321 ggcacgcgtt gatagcttat tggcacttct cccgttcggc ttcacgactt tgataaggaa
     1381 tcttgccatg ccccagggta aagaagtatt cttgggccta aatcagaccg gcgtgatcgt
     1441 gtttatcgtt gccgggctga tctgcttccc gatcggcttc atcgtgccgt tcttcgtcga
     1501 ttcttgtaag tcggacccga acgtaaagta gcggtgcagg aacgagccca agccttgcga
     1561 tccggcatgc tagcgggccg cgattcctgt tggtgaatcc gcccgcccgg ccgtagaatc
     1621 cgccgcgggg ttttaccgca ttcgccccgt cacggatgac gacccatggc cgcactccct
     1681 gtgcccgcgc tggaggcgcg cagccccttc gagcagcttc gcctcgcccg gcagatcgtg
     1741 cgcgccgagg gccagacgtt gctcgcgctg gccgagcgcc tgggcgacga gttctgccac
     1801 gccattggcg tactaagtgc cgcccaagga agcgtcatcg tcagcggcat ggggaaggct
     1861 gggctgatcg gccagaagat cgccgccacg ctcgcttcga ccggcacgcg gagccatttc
     1921 gtccacccgg ctgaggccat tcatggcgac ctggggcgga ttcacgcgga cgacgtcgca
     1981 ctcgtcttgt cgttcagcgg caatacagaa gaaatcgtcc gcatcctgcc ggccctaacg
     2041 cagctcggcg tgccggtcgt ggccgtcacc ggccagccgg acagtccgct cggcagagcc
     2101 gcggcggtga cgctcgacct gggcccgatt cgcgaggcct gtccgcacgg cctcgcgccg
     2161 accgccagca cgacggcgat gttggccctg ggcgacgcac tggccctggt cctcagccgg
     2221 atgcggggct tcacgcccga cgacttcgcc cgcttccatc ccggcggcaa tctgggccgc
     2281 aagctggccc gcgtggaaga cgcaatgcgc ccgctaggcc agtgccgcgt cgctaagcaa
     2341 acccaaacgc tccgcgagac cctcgtccac caaagccgtc ccggccgccg caccggcgcg
     2401 atcatgattg ttgatgacgc aggcaaactc gccggcatct tcaccgacag cgatctggcc
     2461 cgcctcctgg aagccaaccg cgacgcggcg atcgacggcc cgctcagcgc ggtgatgacg
     2521 cagagcccgt ccaccgtcct cgccggtcag cccctttcgc acgcctgcga cctgctggcc
     2581 cagcggaaga tcagcgagct tcccgtcgtc gattccgtcg gccagccggt aggattgatc
     2641 gacatcaccg acgtgctggg cgccgagccg gaagccggcg aagtccaaag tccaaagtcc
     2701 aaagtccaaa gtcgcaccgg cgccttcaaa ctcctgactc ctgattcctg acccctgaac
     2761 cctgacccct cccccatgtc cctcgccgac ctttgccgcc cgatcgagct gatcctctcc
     2821 gacgtggatg gcgtgctgac cgacggcggc atctggtacg acaaccaggg ggtcgagctc
     2881 aagggctttc acatccgtga cggcctgggg atcaaagtct ggcagcgggc cgggttcaag
     2941 ttcggcgtgc tgaccgcgcg gacttcccac attgtcaagc tgcgggccgg cgagctggga
     3001 atcgacctcg tgcggcaggg ctttgaagac aagctgccgg tggctcagga aattctccgc
     3061 gagcggggct tgtcgcccga gcaggtctgc tacatcggcg acgagttgac cgacctgccg
     3121 gtgatccgct tcgtcggctt gggcgtggcg gtggccgacg ccgccgagga agtgcgggcc
     3181 gccgcggccc atacaacccg cacctccggc ggccacggcg ccgtccgcga gctggtcgag
     3241 acgatcctca aggccaaagg ccggtgggac gacgttattc aaaggtacct ggcgtaaacg
     3301 tcagtggtca gttgtcagtt gtcagtgggg actcctgctg actacggacc actgacaact
     3361 gaccactgac aaatcgtgct tcacttccgc cgttacatcg ccgcgctggc cgtgctcctg
     3421 gcagcattcg gcttgtacta cgtgctggtg gcgccgtggc tggagccgcc gcctatcggc
     3481 cggccgccgg tcgcggatat tggaccggtg cagccttcgc ccggcgtgga atccgagctg
     3541 gcggaactct tcccgcccga tgcctgggag cgcgacccac aaacgaaggt cgtcgaaacg
     3601 agccaatgca tcctgctcgt gaaggactat ctgccgacgc ccgacggccg cttggagctc
     3661 aatccctgca cgctcgtttt ctacaccgag ggcgacaccg ccagcttggc cggcggcact
     3721 gccatggcgg gcggggggac gaaccggcgg ccgatcgtcc tgcaagcgcc ggagggagcg
     3781 gtcctgatct tcgaccggcc gctggaaatc ggccgcgggc agttcggcca gattgtcggc
     3841 ggcaagctgg agggcgaggt gaccatcagc agcccaccct cgcagccgga cggagacgac
     3901 gccctgcgcc tggtgacgcg caacgtgcag ctcgccaaaa accgcatttt cacgccgggc
     3961 gacgtcgcta tt
//
LOCUS       DASUGB010000919         4860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_797266, whole genome shotgun sequence.
ACCESSION   DASUGB010000919 DASUGB010000000
VERSION     DASUGB010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797266"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFV87_17440"
     CDS             <1..184
                     /locus_tag="VFV87_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105609.1"
                     /translation="DITALVCQGLSPFDAAVLGAHVHGLAGDLAAAELGQVSLIASDL
                     LKYLPKAFQAVGERGT"
     gene            251..1201
                     /locus_tag="VFV87_17445"
     CDS             251..1201
                     /locus_tag="VFV87_17445"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5105610.1"
                     /translation="MQLLHSLDNLPADLRQGAVSIGNFDGVHRGHAKLVERLLAKARE
                     VGGPAIVFTFDPHPVRLLRPDRVPPPLTWTDRKAQLLDELGVTAIIAYPTDLDLLQLP
                     PEGFFDKIIRQQLAARAMVEGPNFYFGSGRTGNIDTLTKLCQQASISLEVVQPTLIGG
                     QIVSSSRVREAIAAGDVDLARQLLTRPYRVRGMVTHGAGRGAKIGFPTANIEAIDTLL
                     PAQGVYAGQALTPGGNWPAALNIGPNPTFGEHAQKVEVHLIDFAGSLYGQPLEVDFFA
                     RLRSIQPFESVAALKSQLARDIAAARQIAAAEPAEDEDAD"
     gene            1282..2262
                     /locus_tag="VFV87_17450"
     CDS             1282..2262
                     /locus_tag="VFV87_17450"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX5105611.1"
                     /translation="MIDWPRFAEIIRSHERFLLVSHIRPDCDALGSELGMAGVLEALG
                     KTVRIVNGQATPPNLAFIDPEQRIGVIGQTVQPTDLADSEVIIILDTSAWAQIGPMSD
                     FIRAFSGKKLVVDHHVSEDDLGAEPFKNTQAEATGRLVVEAAEALGVKLTPEMAVPLF
                     AAVATDTGWFRFASASSGTYRVAAKLIDAGADPAAIYKALYEQDTLGRMKLRGRILSR
                     IETDLSGRLAHTFVLKSDFAETGSLPSDTEDMVNFCLAIKGTEVAVILVEQQSGGFKI
                     SFRSRSQVDCSKLAEVYGGGGHKAAAGAFIKGPLAEVQPLVLDAVRRAMG"
     gene            2378..2995
                     /locus_tag="VFV87_17455"
     CDS             2378..2995
                     /locus_tag="VFV87_17455"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5105612.1"
                     /translation="MLKSLKPTTTPQVSPTPAPPEPRRRGFLAGAAALIIGGLVGLIP
                     MGAGLFVFLDPILKRKAKGDGKGQEDSRPYRRVASLDALPMDGTPVQVPVIADLTDAW
                     NREPNQPIGAVYLRRTGKESVECFNAICPHAGCFVGYAGDRKCFQCPCHTSSFQLNGS
                     RIMPSPSPRDMDALKVDDEKLKNDGEVWVQFVNYYPGHAEREAKP"
     gene            2992..>4860
                     /locus_tag="VFV87_17460"
     CDS             2992..>4860
                     /locus_tag="VFV87_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX5105613.1"
                     /translation="MIGRLRDWINHRIGLQDIVQHALYEPIPSGARFRYITGSVLVFC
                     FATQAITGIFLWMAYSPSSQTAYESTYYIQHEMAGGWLVRGIHHFTAQAMVVVMALHM
                     LQVVWDGAYRAPREVNYWLGLVLLQLVLGLGLTGYLLPWDQKGYWATNVATNLATLVP
                     VVGKDIQQLAVGGNEYGHHTLTRFFALHAGVLPALLVIFLALHIAIFRRHGIMAKITP
                     GRADDTFWPRQVLFDAIGCLVLLAVVLSLTIHFDFGGALSGNLPPEHRGAELGAPADP
                     SEAYSAARPEWYYLFLFQLLKYFPGSLEIIGALVIPGVVMTILFLLPFIGRSYWGHQF
                     ARAFMVLLLVGAAVLTGMALADDYYAALGPKLGLKDEKKMQASHEFMQAREEAEHDAR
                     RTDELIKRFKLNEDGEPTDPLMIPKHGAVYLLRNDPKTRGPRLFKQHCASCHSYYNPA
                     DPEGKDGPQFLITQGPQTQADPKNKDRQIVVRGADEKVPYDPVPDAAGAPNLYDFASR
                     AWIRGLLETAVDKSNPDKKLPPNISAPVVIGEPRPSSNPSVAADPENPQNYNRPLFSD
                     YFGNTNHKAGRMAAWVAKHADLLNANADAIAAALSAQAMLPDQAEEDQADTALIQKG"
BASE COUNT          874 a         1570 c         1524 g          892 t
ORIGIN      
        1 cgatatcacg gcgctcgttt gtcagggcct gtcgccgttt gacgccgcgg tgctcggggc
       61 acatgttcac ggcctcgcgg gcgatctggc agcggctgaa ctgggccaag tgtccctgat
      121 tgcgagcgac ttgctaaagt acttgccgaa ggcgtttcag gcagttggag agcgggggac
      181 atgagtcccc tggggccagc cgcctccacg atctcagcgg gctgacgccc gccgctcgcc
      241 agaatcaatc gtgcaactac tccattctct cgacaacctg ccagccgatc ttcgccaggg
      301 agcggtttcg atcggcaatt tcgacggcgt gcaccgcgga catgcgaagc tggtcgagcg
      361 attactggcg aaggcccgcg aggtgggcgg gccggcgatt gtgtttacgt tcgatccgca
      421 cccggtgcgg ctgctacggc ccgatcgcgt gccgccgccg ctgacgtgga ccgatcgcaa
      481 ggcgcagctt ctggatgagt tgggggtgac ggcgatcatc gcctatccaa ctgatctgga
      541 tttgctgcag cttccgccgg agggcttttt cgacaaaatc atccgccagc agctcgcagc
      601 ccgggcgatg gttgaagggc cgaatttcta ctttggcagc ggccggacgg gaaacatcga
      661 cacgctcacg aagctctgcc aacaggccag tatctctctc gaggtcgttc agccgacgct
      721 gatcggcggg cagatcgtct ccagcagccg cgtccgtgag gcgattgcgg cgggtgacgt
      781 cgatctggct cggcagcttc tcacgcggcc gtatcgcgtc cgcggcatgg tgacgcacgg
      841 cgccggccgc ggggcgaaaa tcggctttcc gaccgccaat atcgaggcca tcgacacgtt
      901 gctgccggcc cagggcgtct atgcggggca ggcgcttacg cccggcggca attggccggc
      961 tgctttgaat atcggtccaa acccgacttt tggcgagcat gcccagaaag tcgaggttca
     1021 cctgatcgac ttcgccggct cgctttatgg ccagccgctg gaggttgatt tcttcgctcg
     1081 cctccgcagc atccagccct tcgagtcggt tgccgccctc aagtcccaac tcgcccgcga
     1141 catcgccgcc gcccgccaaa tcgcagccgc cgagcctgcg gaggacgagg acgcggattg
     1201 acagaaaccg gtccaaagtc caaagtccaa ggtcatgcga ttgccgcctc ggactttgga
     1261 ccttggactt tggacctgcc catgatcgat tggccccgct tcgctgaaat catccgctcg
     1321 cacgagcgat ttctgcttgt cagccatatc cgccccgatt gcgatgcgct gggcagcgag
     1381 ctgggcatgg ccggcgtgct ggaggcgctc ggcaagaccg tgcgaattgt caacggccag
     1441 gcgacgccgc cgaacctggc gttcatcgac ccggagcagc gcatcggcgt gattggccag
     1501 accgtgcagc caacggattt ggcggacagt gaagtcatca tcattctcga caccagcgcc
     1561 tgggcccaga ttggtccgat gagcgacttc atccgcgcgt tctccggcaa gaagctggtc
     1621 gtcgatcatc acgtcagcga agacgatctg ggcgcggagc cgttcaaaaa tacgcaagcc
     1681 gaagcgaccg gtcggctggt ggtggaggcg gccgaggcgc tcggcgtgaa gctgacgccg
     1741 gaaatggcgg tgccgctgtt cgcggcagtg gccaccgaca ccggctggtt ccggtttgcg
     1801 tcggcttcga gcggcacgta ccgcgtggcg gcgaagctga ttgacgccgg tgccgatccg
     1861 gcggcgattt acaaggcgct ctatgagcag gacacactcg gccggatgaa actccgcggg
     1921 cggattctgt cgcggatcga aaccgacctc tccggccggc tggcgcatac gttcgtgctg
     1981 aaatcggact tcgcggaaac cggctcgctc cccagcgaca ccgaggatat ggtcaacttc
     2041 tgcctcgcga tcaagggcac cgaagtcgcc gtgatcctcg tcgagcagca atccggcggc
     2101 ttcaagatca gcttccgcag ccgcagccaa gtcgattgca gcaagctggc cgaagtctat
     2161 ggcggcggcg gtcacaaggc ggcagccggg gcgttcatca aagggccgct cgccgaggtg
     2221 cagccgctcg tccttgacgc cgttcgcagg gcgatgggat aatcgagcgt cgggattggc
     2281 gggcatgact gctcgcggac ccgatcccgc cccagctccc gcctctgact ctgttatcat
     2341 cggcaagagg ccgagcctct cacccggagt cccgatcatg ttgaagtcgc ttaagccgac
     2401 gacgacacct caagtttcgc ccacccccgc cccgcccgag ccgcggcggc gcggcttcct
     2461 cgctggcgcg gcggcactga tcatcggcgg cctggtcggg ctgattccga tgggcgcggg
     2521 actgttcgtc tttcttgatc cgatcctcaa gcgcaaagcc aaaggggacg gcaaaggcca
     2581 agaagattcc cgcccgtacc gccgcgtggc gtcgctcgac gccctcccca tggacggaac
     2641 gcccgtgcaa gtgccggtca ttgctgacct gaccgacgcc tggaaccgcg agcccaacca
     2701 gccaatcggc gcggtctatc tccgccgcac cggtaaggaa tcggttgagt gcttcaacgc
     2761 catctgcccg cacgctggct gctttgtggg ctatgcgggg gaccggaagt gctttcagtg
     2821 cccctgccac accagcagct tccagctcaa cggcagccgg atcatgccca gccccagccc
     2881 gcgcgacatg gacgcgctca aagtggacga cgagaagctc aagaacgacg gcgaagtctg
     2941 ggtccaattc gttaattatt atcccgggca tgccgaacgg gaggccaagc catgatcggc
     3001 cggctgcgcg attggatcaa ccaccggata ggcctccagg acatcgtgca gcatgcgctg
     3061 tacgagccga ttcccagcgg ggcccgcttc cgctacatca ccggcagcgt gctcgtgttt
     3121 tgcttcgcga cgcaggcgat caccggcatc ttcctctgga tggcctacag cccttcgagc
     3181 cagaccgctt acgagagcac ctactacatt cagcacgaaa tggccggcgg ctggctggta
     3241 cgcggcattc atcacttcac ggcccaggcg atggttgtcg tgatggccct gcacatgctg
     3301 caggtcgtct gggacggggc ttaccgcgcg ccgcgcgagg tgaattactg gctgggcctg
     3361 gtgctattgc agctcgtcct cggcctgggc ctcacggggt atctcttgcc gtgggaccag
     3421 aagggctact gggcaacgaa cgtcgcaacc aatctcgcca cgctggtgcc agtggtcggc
     3481 aaagacatcc agcagctcgc cgtcggcggc aatgagtatg gccatcacac gctgacgcgg
     3541 ttcttcgccc tgcacgctgg cgttttgccc gcgctgctcg tgatttttct cgccctgcac
     3601 atcgcgatct tccgccggca cggcatcatg gccaagatca cccccggccg ggctgacgac
     3661 acgttctggc cgcggcaagt gctgttcgac gccatcggct gcctggttct gctggcggtt
     3721 gtgctgtcgc tgacgattca tttcgacttc ggcggagcgc tgagcggcaa tctgccgccg
     3781 gaacatcgcg gggcggagct cggcgcccca gccgatccgt cggaagcgta ttccgccgcc
     3841 aggccggaat ggtattacct gttcctgttc cagcttctga aatatttccc cggctcgctg
     3901 gagatcatcg gggcgctggt cattcccggc gtcgtgatga cgatcctgtt cctgctgccg
     3961 ttcatcggcc ggagttactg gggccatcag ttcgcgcggg cgttcatggt cctgttgctc
     4021 gtcggcgccg cggtcctcac cgggatggct ttggcggacg attactacgc cgccctcggt
     4081 ccgaagttgg gactgaaaga cgagaaaaag atgcaggcct cgcacgagtt catgcaggcc
     4141 cgcgaagagg ccgagcacga cgctcgccgc accgacgagt tgatcaagcg attcaaactg
     4201 aatgaagatg gcgaacctac cgatccgctg atgattccca agcacggcgc cgtctatctg
     4261 ctgcggaacg atcccaagac ccgcggtccg cggctgttca agcagcactg cgcgagctgc
     4321 cacagctatt acaacccggc cgatcctgag ggaaaggacg gcccgcagtt tctcatcacg
     4381 caagggccgc agacgcaggc agacccaaag aacaaagacc ggcaaatcgt cgtgcgtggt
     4441 gccgatgaga aggttccgta cgatcccgtc cctgacgcgg ccggcgctcc gaatctttac
     4501 gatttcgcgt cgcgggcgtg gattcgcggg ttgttggaaa cggctgtgga caagtcgaac
     4561 ccagacaaga agctcccgcc gaacatctca gctccggttg ttatcggcga gccgcggcct
     4621 tcgagcaatc ccagcgtcgc cgccgatccg gagaatcctc agaactacaa ccggccgctt
     4681 ttttccgatt acttcggcaa cacgaatcac aaagccggcc gaatggccgc gtgggtcgcg
     4741 aaacacgccg atctgctgaa tgcgaatgcg gacgcgattg ccgccgcgct gtccgcccag
     4801 gccatgctgc cggaccaggc ggaagaagac caggctgata ccgcgctgat tcagaaaggg
//
LOCUS       DASUGB010000920         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_102107, whole genome shotgun sequence.
ACCESSION   DASUGB010000920 DASUGB010000000
VERSION     DASUGB010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102107"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VFV87_17465"
     CDS             complement(<1..1567)
                     /locus_tag="VFV87_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105614.1"
                     /translation="MNSSVLQACHRSVFVLGTWVLAWAACQAAPVPGEGRVQLMLDLQ
                     GIRGPVADVAFSSDEKHVAACGGKAVRVWDLETGQLHYTLRGQIDVGPFGDCLALAFS
                     PDNCFLAVGISNHTEWGSIRIYDTHDFTQIKELLPGHRLPVTRLAFSHDSRYLAAADS
                     SGAVSIFDWPLRRIVATVRPTDHANGEYAAFHFPTDELFLLVRGKAEEAVIAVPSGKL
                     LDDRRHEPAAVTAWRRSHAQSEAPLGVAGRFDLRLTRQTWMAAGIGREGSGSAFWAAC
                     WNSAGERPRCMYRGHQRRITALALSQSCRWAASGQDDGAVHVWDASTGQPRYVFRNTG
                     QPCYRAGLDLATRTIGLAPTPLQGANWAFNSFGRIDRVFDFNRRSVTGMPENAVFPAE
                     FTEHAHGRLTLTFEQQKYGIQLAGANGATASFELPTDASPTCFTLLRHQQMGIANPVV
                     VGDKSGRLVCYDPATRQPRRQFTGHTDFVTSLSETPDGQYLISSSTDGTIRVWSLAQR
                     GERAWIDFQLTGDV"
     gene            complement(1631..>2780)
                     /locus_tag="VFV87_17470"
     CDS             complement(1631..>2780)
                     /locus_tag="VFV87_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105615.1"
                     /translation="AGCQLPLVDVDSAELMRLMVVQALAAEIQVRILDAIRSGDLAAA
                     QQLWETHCPGAAAPFRRSRFCTRFASFCSRLEAGACTPQEAVDMEREMLALGLAEHPC
                     CGAPSLLEEIRECVLIGQRIGAARPGVQTQAAILPPGEIDLIYNPGVADGEAIVLDAG
                     SVMLGTGGIGTMHVQHGNIAQQQGLPLGTGISVAEDDTKLVNTGFLIENTTQSPVNFV
                     LANQQFELAPLTRQSYSAGPTEISFYTGASRQLARHSVKPGTLRFVVERGGFAVRAVT
                     AFQATIDNSANDSPFHYIFQGADAVCPPRSKQSHESLYPLVFRFDRGNGSVTKQVVSR
                     ERQTVLAVGINPSDNLWDLFAPGDLPASRPVILPQGDTSDSVPLFLNL"
BASE COUNT          521 a          876 c          898 g          485 t
ORIGIN      
        1 cgacgtcgcc tgtgagttga aaatcgatcc acgccctctc gccgcgctgg gcgaggctcc
       61 acacgcgaat cgttccgtcc gtggagctgc tgatgagata ctggccgtcc ggagtttccg
      121 acagcgacgt aacgaagtcg gtgtggccgg tgaattgccg ccgcggctgg cgcgtggccg
      181 gatcgtagca caccagtcgg ccgcttttgt ctcccacgac aacagggttc gcaattccca
      241 tctgctgatg acgcaagagc gtgaagcaag tgggagaagc gtcagtcggc agctcaaacg
      301 acgccgttgc cccgttggcg ccggcaagct ggatcccgta tttctgctgc tcaaacgtca
      361 gtgtgagccg gccgtgggcg tgctcggtaa attcagcggg aaagaccgcg ttttctggca
      421 ttccagtcac gctgcggcga ttgaagtcga agacgcggtc gatcctgcca aagctgttga
      481 atgcccagtt cgcgccttgc agcggcgtcg gcgcaaggcc gatcgtgcgc gtggccaggt
      541 ccaggccggc ccgatagcac ggctgaccgg tgttgcggaa cacgtaccgc ggctggccgg
      601 tggacgcatc ccacacgtgg acagcgccgt catcctggcc tgaagccgcc cagcggcagc
      661 tttggctgag cgccagcgcg gtaatccgcc gctggtgccc gcgatacatg cagcgggggc
      721 gttctccagc actgttccag caggctgccc aaaaggcgct gcccgaacct tcccgtccga
      781 tgcccgcggc catccacgtt tgccgcgtaa gccggagatc gaagcgacct gcgacgccca
      841 gcggagcctc ggattgggcg tgtgacctgc gccaggcggt gacggccgct ggctcatgtc
      901 ggcggtcatc gagcagcttc cccgacggca cggcgatgac tgcttcctca gcctttccgc
      961 gcaccaggag aaagagctcg tcggtgggaa agtggaaggc ggcgtactcg ccgttggcat
     1021 gatctgttgg ccgcacagtg gcgacaatcc gtcgcaaggg ccagtcgaag atcgagactg
     1081 caccagacga atcggctgcg gcgagatagc gcgaatcatg tgagaacgcc aggcgcgtca
     1141 cgggcaggcg atgaccggga agcaactcct tgatctgcgt gaagtcgtgg gtgtcgtaaa
     1201 tgcggatcga gccccattcg gtgtggttgc tgatgccaac cgcgagaaag cagttatctg
     1261 gagaaaatgc caaggcgagg cagtcgccaa acggccccac gtcgatctgt ccgcggaggg
     1321 tatagtgcag ttgacccgtt tcgaggtccc aaacgcggac cgcctttccg ccgcacgctg
     1381 ccacgtgctt ctcgtcggaa ctaaaagcga cgtcggcgac cggcccacgg attccctgca
     1441 ggtcgagcat caactgcact cgtccctcac ccgggacagg agccgcctgg cacgccgccc
     1501 aggccaggac ccacgttcca agtacgaata ccgagcgatg gcaggcctga aggacggagg
     1561 aattcatggc attctcggct gggttacaag aggcttgtga acgggcttcc gcctggatgg
     1621 cgatcagcgg ctagaggttc aggaagagcg gcacgctgtc cgatgtgtcg ccttgcggca
     1681 gaatgaccgg tcgcgatgcg ggcaagtcgc ccggtgcgaa caagtcccag aggttgtcgc
     1741 tggggttgat gcccacggcc aagaccgttt ggcgctcgcg cgagacgact tgcttggtga
     1801 cggagccgtt tccccggtca aagcggaaga cgagcggata caagctctcg tgcgactgct
     1861 tcgagcgcgg cggacagacg gcatctgcgc cttggaagat gtagtggaag ggggagtcgt
     1921 tcgcgctgtt gtcgatcgtc gcctggaacg ccgtgaccgc ccggaccgca aatcccccgc
     1981 gctcgacgac aaaccgcagc gttccgggtt tgacggagtg ccgcgccagt tggcgactgg
     2041 cgccggtgta aaacgagatc tcggtcggac cggcggaata actctgtcga gtcaagggcg
     2101 ccaattcgaa ctgctggttg gccaatacga aattcaccgg cgactgcgtc gtgttttcaa
     2161 tcagaaatcc ggtgttgacc agcttcgtgt cgtcttccgc cacactgatt cccgttccca
     2221 gcggaagccc ctgttgctgc gcaatgttgc cgtgctgcac atgcattgtc ccgatgccgc
     2281 cagtgcccag catcacgctc ccagcgtcca ggacgatcgc ttcgccgtcg gccacgccag
     2341 ggttgtagat cagatcgatt tcgccgggcg gaagaattgc ggcctgcgtc tggactcccg
     2401 gccgagccgc gccgatccgc tggccgatga gcacgcattc gcgaatctcc tcaagcagac
     2461 tgggcgcccc gcagcacggg tgttcggcca accccagcgc gagcatctcg cgctccatgt
     2521 ccacggcttc ctgcggcgtg caggcgcccg cttccaaccg agagcagaag ctggcgaagc
     2581 gcgtgcagaa cctggaccgg cgaaacggcg ccgcggcgcc tggacaatgc gtctcccaaa
     2641 gctgctgggc cgccgcgagg tctccgctgc gaatcgcatc cagaatgcga acttggatct
     2701 cagcggccag ggcctggacg accatcagcc gcatcaattc tgcggaatcg acgtccacca
     2761 acggaagctg acaaccagcc
//
LOCUS       DASUGB010000921         6151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2048453, whole genome shotgun sequence.
ACCESSION   DASUGB010000921 DASUGB010000000
VERSION     DASUGB010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048453"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3400)
                     /locus_tag="VFV87_17475"
     CDS             complement(<1..3400)
                     /locus_tag="VFV87_17475"
                     /inference="COORDINATES: protein motif:HMM:NF020855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105616.1"
                     /translation="MKRYALSLPVLLITLASAAAIGGLLFGLHQWQLSRISAGLLRHA
                     EQAEAKEDWDRSAEFLDRYLRVHPGDAVVRGQLALTYSKGAQSADDKRMAVELCYRAL
                     GAGNLPDEPKIRSELAGLLIDTGRFLEAEAEAAKVLQFGDASSRPAAQRALALALFGQ
                     LQQGSVSAERTKSLGIVAATESACRINPQDAILAEVAATLFREHAAIVQAELTELQEA
                     ERIQRADGFLDELVLQAPSDPAAYLARYRYRMTYGVPGAAEDLEKAVTLGPDDATVLL
                     TAAVDAMRVARAGETSGNSQQAADNYAAATAYYNKLLEKNFFPQSPEPHLGLGDASLK
                     SASLNDALKAWRDGLKRFAQPTAKAAFHSRIADALIEAGRDREASESLSAIDEMLAAI
                     GSDSSDEQLALLQAQDLRRAALFMKQEQYQAAIPLLQSVLTRESKGKPAAELASFCWN
                     QLGRALAACDQWGDAAVAFDRAVALHPQKAETRLAAALAWFNAGRPDLAAERGDAAVA
                     ASSTFEAWMLTAAVQLQLQITAPLSERTWRRLDAALKALDQAPADTPRRWRIDLLKAE
                     YLAFRARTEGDAEAGKQQAKALLEAAVARQTDAPELLSRACLALENLGFSADADRMLD
                     RMRDAGAPLVEVAVTRSRLLAVRKQFGPAREALEKVALATGAADRARIQRELLRIADA
                     SGDVPLVTRLLESEIAAQPTDLRALRQRCELAVEQKDLSGLKTWRGKLAALGPVGQLW
                     SQYYDVLGLWMLAKDRPNELPTARAACEQLASAKPGWGPSLVLRGMIEQSAGNLDEAA
                     WAYEGAIESGIADTSVFERLIAVLERRNRYADAERYLSRLESKLPFSQRLTELAGGRE
                     LRRDRPERAIEIARDAVAERPEEAAARVWLGRLLLLSKQLTEAEQQFRRAVELAPGDI
                     RSWNGLFSFYVRTGDLDRARLTLDELIKKANLEPRERDIVVAQGLEQLGEFEQAAQKY
                     EATAKRFPENVEIQIRLARLYLRTDPDRAIKCLELALEWDPGSAAARRMLAALLASQG
                     TEESLQRAESLLSQQTGNGADLSLEDRRLHALLLAQNGNNERLQQAIELLEEIIASGK
                     DNAAGDNLVLAQLYEQQSRRMTDEQQAAARMKAARQQLVAVAARP"
     gene            complement(3404..5593)
                     /locus_tag="VFV87_17480"
     CDS             complement(3404..5593)
                     /locus_tag="VFV87_17480"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX5105617.1"
                     /translation="MSDPTQKSAAAAAEGGSQFRVQTIFIALRCWWKIAAPLGIVLAV
                     SAAAGVYFFSKPQYTASAWLIIRDRPPFLLESQFAEDPKKAVENELELMRSPPVIDPV
                     ASIPAVASTPEIARERHPAQALRKLLKIRALGRSDYFVIEFTSEDPERAALIVNEVAK
                     AYLRLHQSTQSERQSLTVKLLRDEQAVQQKWVEQYRANVRELTKQLTGTEAFPAAPNA
                     NVVQIRDATSELQSKLVSAEVDQVILKAQVEAEKELLSKPAPEPLDSAIDQQVEASQP
                     VLALRTRIAESKALMRQHEESSANLPKNVLYQTLKADLAKDEAELEKLRVNLRDEAKE
                     YVKAHTKAQSEAALRDLEQRLANASFTVDVLKERLQNETKDRRTQNGETVDLEFVRAD
                     YERASRVYEAISNKILSMQMEQRAPSPVEIFQEADPPPSPDGEEPFKKMAIFALGALF
                     VPFGLAVFVEHLYRRVSSRDQLETGSGIHVVGEVTSLPRRHSRKSQAKSASRELQLFE
                     ESIDGLRTYLKLVHSVQGMQVLAVTSAVSREGKTSLASQLAISVARSTGETTLLIDGD
                     MRSPDIHRAFDIDRGPGLVDVLKGTATLQQAINTDFSDKLHLLPAGSLSSSPHKVLGT
                     NEFQAILEGLKGSYRHILIDTPPLLPASEALLLARAADAAILCVRRDYSRVDQTVEAF
                     GRLRDAGIKVAGAVLNGIPARVYVYRYGSYYAESQRLDGSPAEDLAT"
     gene            complement(5604..>6151)
                     /locus_tag="VFV87_17485"
     CDS             complement(5604..>6151)
                     /locus_tag="VFV87_17485"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX5105618.1"
                     /translation="SVPAIGFLFFMIPLPHALAGEMSYPLQKIATRLSCLGLQTLGQP
                     AFADGNVILLNDQQFEVAQACSGLRLFVSILALTYAYVAIISRPWWEKLGLCLIAIPV
                     AILSNAARIIATALLYELTTSETLRQWAHDSAGWGTILFAGMLLAAALYYFRWLIREE
                     ELMDLASLVRRSDVPRLGAES"
BASE COUNT         1112 a         1921 c         1903 g         1215 t
ORIGIN      
        1 tgggacgcgc cgctacggcg acaagctgct ggcgcgccgc tttcatgcga gcggcagcct
       61 gctgctcgtc agtcatgcga cgagactgct gctcataaag ctgagcgagg acgagattgt
      121 cccccgccgc attgtctttt ccgctggcaa taatctcttc cagcaactca atggcttgct
      181 ggagccgctc gttgttgcca ttctgagcca acaaaagtgc atgaaggcgg cggtcttcca
      241 gcgacaagtc cgctccattg ccagtctgct gactcaacag cgattcggcc cgctgcaagg
      301 actcctcggt cccttgagag gccaacagcg ccgccagcat tcggcgggct gccgcggagc
      361 ccggatccca ttcgagggct agttccaggc acttgatggc tcgatccgga tcggttcgca
      421 aatacaggcg cgccaggcga atctgaatct ccacattttc cgggaagcgc ttggcggttg
      481 cttcgtattt ctgcgcggct tgttcgaact ccccgagctg ctccagtcct tgcgcgacta
      541 cgatgtcgcg ctcccgcggc tccaggtttg ccttctttat cagttcatcg agcgtgagcc
      601 gcgccctgtc gagatcgcca gttcgaacgt aaaaggagaa cagaccattc caactgcgga
      661 tgtcacccgg agccaattct acagcgcggc ggaattgctg ctctgcttcc gtcaactgct
      721 tgctcagcag caataaccgg ccgagccaga cgcgagctgc ggcttcttcc ggacgttcgg
      781 cgacggcatc gcgggcgatt tcgatggccc gctcagggcg atcgcggcgc aattcccggc
      841 cgccagccaa ctctgtcagt cgttgcgaaa acggcagctt cgactccagc cgggaaagat
      901 atctttccgc gtccgcatag cggttccttc gctccagaac ggcgatcagc cgctcgaaga
      961 cgctcgtgtc tgcaattcca gattcgatcg caccctcgta cgcccacgct gcctcgtcca
     1021 gatttccggc actctgttcg atcattccgc gaagcaccag cgaaggcccc cagccgggtt
     1081 tcgcggacgc cagttgctca caggctgcac gcgcggtggg caattcgttg gggcgatcct
     1141 tcgccagcat ccataggcct aggacgtcgt agtattgaga ccacagttgt ccgactggcc
     1201 ccagagcggc cagcttgccg cgccaagtct taagccccga aaggtctttc tgctcgacgg
     1261 ctagttcaca gcgctgccgc aatgcccgca ggtcggttgg ctgggcggcg atttcacttt
     1321 ccaacaggcg cgtcacgagc ggcacgtcac cggatgcatc ggcaatccgg agcagttcgc
     1381 gctgaattct ggcccgatcg gccgcgcccg ttgccagggc taccttctcc agcgcctcgc
     1441 gcgccgggcc gaattgcttg cgcaccgcga gcagccgtga acgggtaacg gcaacctcaa
     1501 ccagcggcgc cccggcatcc cgcatgcgat cgagcattcg gtccgcatcg gccgaaaagc
     1561 ctaggttctc cagagccagg caagcgcgcg acaaaagctc cggcgcgtct gtctgtctgg
     1621 cgacagctgc ctcaagaagc gctttggctt gttgtttgcc cgcctccgca tcgccttcgg
     1681 ttcgcgcgcg aaacgcgaga tattctgctt tcaacagatc aatccgccag cggcgcggtg
     1741 tatcggcagg cgcctgatcg agcgccttca gggcggcgtc taagcgtctc caggtgcgct
     1801 cggaaagcgg cgcggtaatc tgaagctgca gttgcaccgc tgccgtgagc atccaggcct
     1861 cgaatgtgct gctggcggcg acggcggcat cgcctcgctc ggcagccagg tcgggccgac
     1921 ctgcgttgaa ccatgctagg gctgccgcca ggcgtgtttc tgccttctgc ggatgcagcg
     1981 cgaccgcgcg gtcaaacgcg accgcagcat caccccactg gtcgcaggca gccaaggcac
     2041 ggccaagctg gttccagcag aacgatgcca attcggcggc tggtttgccc ttcgactctc
     2101 gcgtcaatac gctctgcaac agtggaatgg ctgcttggta ctgctcctgc ttcatgaaca
     2161 aagctgcacg gcgtaggtcc tgagcttgca ggagcgccaa ttgctcgtct gaagaatcgc
     2221 tgcctatagc agccagcatt tcatcaatcg cgctgagcga ctcactcgct tcgcgatcgc
     2281 gcccggcctc gatgagagcg tcggcgatgc ggctgtggaa cgcggctttg gctgtcggct
     2341 gggcaaaccg tttaagtcca tcgcgccacg ccttcagcgc atcgttcagg ctggcactct
     2401 tgaggctggc atcgcccagg cccaaatgcg gctcggggct ctggggaaag aagttctttt
     2461 cgagcaactt attgtaataa gctgttgccg cggcgtagtt gtctgccgct tgctgagaat
     2521 tgccgctggt ctcgccggcg cgggcgactc gcatggcatc cacggcagcg gtgagcagca
     2581 ccgtggcatc gtccggaccc agggttacgg ccttctcaag gtcctcggct gcgcccggca
     2641 ctccgtacgt catgcgatag cggtaacgag ccaaataagc agcgggatcg ctcggtgctt
     2701 gaagcacgag ttcgtcaagg aatccgtcgg ctcgctgaat tcgctcggcc tcctgcagtt
     2761 cagtaagctc agcttgcacg atcgccgcgt gctcccgaaa gagcgtggca gcaacctccg
     2821 ccaggatggc atcctgcgga ttgattctgc aggccgattc cgtggcagca acgatgccga
     2881 gcgacttggt ccgctcggcc gacacactcc cctgctggag ctggccgaac agcgcgagcg
     2941 ctagggcccg ctgggcagcg ggacgcgagg acgcatcgcc gaactgaagg accttggcag
     3001 cttcagcttc ggcttcgagg aaccggcccg tatcgatgag caagcctgcc aactcagacc
     3061 gaatcttggg ctcatcgggc aaattgccgg cgcccagcgc gcggtagcac aattcgaccg
     3121 ccatgcgttt gtcgtcagcc gattgcgctc ccttggagta cgtcagggca agctgtccgc
     3181 gaacaactgc atcgccggga tgcacccgca aataccggtc gaggaattcc gcggagcggt
     3241 cccagtcttc cttggcttcc gcctgctcgg cgtggcggag caatccggcg gagatgcgcg
     3301 aaagctgcca ctggtgcagg ccaaaaagca acccgccaat ggcggctgcc gatgccagcg
     3361 tgatcaacag gacggggaga ctaagtgcgt agcgcttcac gaattatgtt gccagatctt
     3421 cggcgggtga cccgtccagc cgctgcgact cggcgtagta actgccgtag cgatagacat
     3481 agacgcgggc ggggatgccg ttgagcacgg cgccggcgac cttgatgccg gcatcacgga
     3541 gccggccgaa agcttcgacg gtctgatcga cgcggctgta gtcgcgccgc acgcacaaga
     3601 tggcggcgtc ggcggcgcgg gcgagcagca gagcctcgct ggcgggcaac agtggcggcg
     3661 tatcgatcaa gatgtgccga tagctgccct tgaggccttc gaggatggcc tgaaactcgt
     3721 tcgtcccgag tactttgtgc ggactggagc taagcgagcc ggccggcagc aaatggagct
     3781 tgtcgctgaa atcggtgtta atcgcctgct gcagcgtagc cgttcctttg agcacatcga
     3841 ccaggcccgg gccgcggtcg atatcgaagg cccggtggat atccggcgag cgcatatcgc
     3901 catcgatcag cagcgtcgtc tcgccggtgg atcgggcgac gctgatggcg agctgcgatg
     3961 ccaggcttgt ttttccctcg cggctgacgg cgctggtcac ggcgaggacc tgcatccctt
     4021 gcacggaatg aaccagcttc aagtaggttc gcagtccgtc gatgctctcc tcgaaaagtt
     4081 gcagctcacg gctggcagac ttcgcctggg attttcggga atgccgccgt ggcaacgatg
     4141 tcacctcgcc cacgacgtga atgccgctgc cggtctcgag ctggtcgcga gagctcacgc
     4201 ggcggtagag gtgctcgacg aacaccgcca ggccaaacgg tacaaacagc gcacccaacg
     4261 cgaaaattgc cattttcttg aatggctcct cgccatcggg gctgggtggc ggatctgcct
     4321 cttggaaaat ctcgacggga ctcggcgccc gctgctccat ctgcatcgaa aggattttgt
     4381 tcgaaatcgc ttcgtaaacg cgggacgcgc gctcgtagtc ggcacggaca aactccaaat
     4441 cgacggtctc gccgttttgc gttcgccggt ccttggtttc gttttgcagc cgctccttca
     4501 gcacatccac cgtgaagctt gcattcgcca gtcgctgttc gagatcgcgg agcgccgcct
     4561 cactctgcgc cttcgtgtgg gccttgacgt actccttcgc ttcgtctcgg agattgacgc
     4621 gaagtttttc cagctcggct tcgtctttcg ccaggtccgc cttgagcgtc tggtagagca
     4681 cattcttcgg aagattggca gacgactcct cgtgctgacg catgagggcc ttcgattcgg
     4741 cgatccgcgt ccgcaaagcg agtacaggct ggctggcctc gacctgctga tcgatggcgc
     4801 tgtccagagg ctccggcgcc ggcttgctga gaagctcttt ttcggcctcg acctgcgcct
     4861 tgaggatgac ctgatcgact tcggccgaaa caagcttcga ttgcagctcc gacgtggcgt
     4921 cgcgaatttg gacgacattt gcgttcggcg ctgccggaaa tgcctccgtc ccggtcaact
     4981 gctttgtcag ttcgcgaacg ttggcgcgat attgttcgac ccacttttgc tggactgcct
     5041 gttcgtcgcg cagcagcttg acggttagcg actgccgctc agattgtgtt gattggtgca
     5101 gccgcagata ggccttggcg acctcgttga cgattagggc ggcccgctcg gggtcttcgc
     5161 tggtgaattc gatcacaaag taatcggatc ggccaagagc tcgaatcttg agcagcttgc
     5221 gaagcgcctg cgccgggtgc cgttcgcggg cgatttcggg tgtgctggcc acggcgggaa
     5281 tgctggccac cgggtcgatg acaggcgggc tgcgcatcag ctccagctcg ttttcgaccg
     5341 ccttcttggg atcttcggca aactggcttt ccagcaaaaa cggcggccga tcgcgaatga
     5401 tcagccaggc ggaggccgtg tattgcggct tggaaaagaa atacacgccg gctgctgcgg
     5461 agacggccag gacgattccc agcggtgcgg cgatcttcca ccagcagcgc agggcgatga
     5521 agatcgtctg cacgcggaat tgcgagccgc cttcggccgc cgcggccgcg gatttttggg
     5581 tggggtcgct catgatcgac gcgtcagctc tcagccccca gccgcggaac gtctgaccgc
     5641 cggacgagcg acgcaaggtc catcagttcc tcttcgcgga tcagccagcg gaagtaatat
     5701 agcgcggcgg caagtagcat gccggcaaac aaaatcgttc cccagcccgc cgaatcgtgg
     5761 gcccactggc gaagcgtctc gctggtcgtc aattcgtaaa gcaacgcagt ggcgatgatc
     5821 cgagcggcgt tggaaaggat ggcgacggga attgcaatca ggcacagccc caatttttcc
     5881 caccacgggc ggctgatgat ggcgacgtag gcatacgtca gcgccaaaat gctgacgaac
     5941 agccgcaatc ccgagcaggc ctgggccact tcgaattgtt ggtcgttaag caggatgacg
     6001 ttaccgtcgg caaacgccgg ctggccgagc gtttgcaggc cgaggcagct tagccgcgtc
     6061 gcgatcttct gcagcgggta gctcatttcg ccggcaaggg cgtgcggtag cggaatcatg
     6121 aagaacaaga agccgatcgc gggcacgctc c
//
LOCUS       DASUGB010000922         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2048554, whole genome shotgun sequence.
ACCESSION   DASUGB010000922 DASUGB010000000
VERSION     DASUGB010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048554"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV87_17490"
     CDS             <1..225
                     /locus_tag="VFV87_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX5105619.1"
                     /translation="LDPIATNKVEDLVRELRGDYSIVIVTHNMQQASRVSDYTAFMYL
                     GRLIEYGPTSEIFTRPKLKETESYVTGRFG"
     gene            complement(241..726)
                     /locus_tag="VFV87_17495"
     CDS             complement(241..726)
                     /locus_tag="VFV87_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105620.1"
                     /translation="MNKQRIAAGTLTVTVALAVALAGDIARAQVNGTESDAEIETLKR
                     RVDQFFGNLTDSAIGPEYAVRDLVGKGPLKDRNDDINKLIEQASGLPQRYGEYTGHEA
                     VGQKAAGKDLVFLRYLYKGEKFPVVWYFTFYRTGPSGSLTRTWQLIGMRFDTKVDGLE
                     K"
     gene            complement(826..>2119)
                     /locus_tag="VFV87_17500"
     CDS             complement(826..>2119)
                     /locus_tag="VFV87_17500"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX5105621.1"
                     /translation="IKAVHLAGTKGKGSTAAMIAAVLTAAGYRTGLYISPHLERIEER
                     IAIDGAICPPERLVVLAARLQAAEACLREGLSPDDVANYPTFFEITTALAFLHFAEAK
                     VDVAVLEVGLGGRLDSTNVCSPEVAVITSISFDHMRQLGNTLAAIAGEKAGIIKPGVP
                     VISGVMTEEPRQVIANRATDLGAQLYQRGGDFDFTRHAGVHIAGAPGSGEVLDYREPG
                     GTAAVELPGLALGMTGEHQAANAACAVAALLRMRERGWTIPEAAIRAGLAAARCPARI
                     EVVPGPPTAVIDVAHNPASIAALVEVLAEQMAGRRKVLIFASSRDKDYAAMLRLLVPR
                     FEAVVLTQYVHNPRAMEAEELASLTRTIAGEQSTALSHPQIHVAQDPPQAWRLAQQLA
                     GPGDAICITGSFFLAAELRGLVLQTSVERCDRPTPSAA"
BASE COUNT          341 a          761 c          629 g          388 t
ORIGIN      
        1 ctcgacccca tcgcgactaa caaggtcgag gacctcgtcc gcgagctccg cggcgactac
       61 tccatcgtca tcgtcaccca caacatgcag caggcctctc gcgtcagcga ctacaccgcc
      121 ttcatgtacc tcggccgctt gatcgagtac ggcccgacga gcgagatctt cacgcgtccg
      181 aagctgaagg aaaccgagtc gtacgtcaca gggcggtttg ggtaagctgc gtgcgcccac
      241 ctacttctcc agcccgtcca ctttcgtatc gaaccgcatg ccgatcagtt gccaggttcg
      301 cgtcaatgag ccgcttggac cggtgcggta gaacgtgaag taccacacga ccgggaattt
      361 ctcgcctttg tacaagtagc ggaggaagac caggtccttt cccgcggcct tctgcccgac
      421 cgcttcgtga cctgtgtatt cgccgtagcg ctgcggcagg ccggacgcct gctcgatcag
      481 cttgttgatg tcgtcgttgc ggtccttcag cggccctttg ccgacgaggt cccgcacggc
      541 gtactccggg ccgatcgcgc tgtcggtcag gttgccgaag aactgatcca cgcgcctttt
      601 gagcgtctcg atctccgcgt cggattccgt gccgtttacc tgcgcccgcg cgatgtcgcc
      661 cgccagcgcc acagcaagcg ccaccgtcac cgtcagagtt ccagccgcga ttcgctgctt
      721 gttcatgatg ctgctcgctt gtagccgcag tcgccagaat gcgggaatcc ggcgacaacc
      781 cgcgttctgg cgaacgcggc taccgaatcc tcaccgagat tctacttacg ctgccgacgg
      841 agtcggcctg tcgcatctct ctacactcgt ctgcagcacg agcccacgca actctgccgc
      901 caggaagaac gagccggtga tgcaaatcgc atcgcccggc cccgccagtt gctgcgccaa
      961 tcgccacgcc tgcggcgggt cttgagcgac gtgaatctgc ggatggctga gcgcagtgct
     1021 ctgctcgccg gcgattgtgc gcgtcaggct tgccaactcc tccgcctcca tcgcccgcgg
     1081 attgtggaca tactgcgtca gcacgacggc ttcgaaccgc ggcaccagca gccgtagcat
     1141 ggccgcgtag tccttgtcgc ggctgctggc gaagatcagc accttgcggc ggccggccat
     1201 ttgttcggcg agcacctcca cgagcgctgc gatcgacgcc gggttgtgtg ccacatcgat
     1261 caccgcggtc ggcggtccgg gcacgacctc gatccgcgcc gggcagcggg ctgccgccag
     1321 ccccgcgcga atcgccgcct cgggaatcgt ccagccgcgc tcgcgcatcc tcagcaatgc
     1381 cgctacggcg cacgccgcgt tcgccgcctg atgctcgccc gtcatgccga gcgccaggcc
     1441 cggcaattcg actgccgcgg tgccccccgg ctcgcgatag tcgagcactt cgcccgaccc
     1501 tggggctccg gcaatatgca ctccggcgtg ccgcgtgaaa tcgaaatcgc ccccgcgctg
     1561 atagagctgc gctcccagat cggttgcgcg attcgcgatc acctgtcgcg gctcctcggt
     1621 catcacgccg gaaatgacgg ggacgccggg cttgatgatc cccgctttct ctcccgcgat
     1681 cgcggccaat gtgttgccta gctgccgcat gtggtcgaag ctgatgctcg taatgaccgc
     1741 gacctcgggc gagcagacgt tggtcgaatc gagccgcccg cccaggccca cctccagcac
     1801 cgcgacatcc actttcgctt ccgcgaagtg caggaaggcc agcgccgtcg tgatctcgaa
     1861 gaatgtgggg taattggcaa cgtcgtcggg cgaaaggcct tctcgcaagc aggcttccgc
     1921 cgcttgcagc cgcgcggcga gcaccaccag ccgctccggc gggcagatcg ccccgtcgat
     1981 cgcgattcgc tcctcgatcc gctccagatg cggcgagata tagagccccg tgcgatagcc
     2041 ggctgcggtc agcaccgcgg cgatcatcgc cgcggtcgag cccttcccct tcgtccccgc
     2101 cagatgcacc gccttgatc
//
LOCUS       DASUGB010000923         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_243324, whole genome shotgun sequence.
ACCESSION   DASUGB010000923 DASUGB010000000
VERSION     DASUGB010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243324"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFV87_17505"
     CDS             complement(<1..225)
                     /locus_tag="VFV87_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5105622.1"
                     /translation="MRFDVLTLFPAIFEGYFGQSLLKKAIAAGLVQVELHDIRRWSRD
                     KHHKVDDRPFGGGPGMILQVEPVVECVEAVR"
     gene            complement(310..753)
                     /gene="rpsP"
                     /locus_tag="VFV87_17510"
     CDS             complement(310..753)
                     /gene="rpsP"
                     /locus_tag="VFV87_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5105623.1"
                     /translation="MAVRLRMKKLGRNKRPFFRICAMDSRAPRDGKVIEELGHYDPLV
                     KDADARAILNGERIAYWLSVGAQPSENVQVLIKKYGKDGTRLAEQQVALERIKTTRPM
                     APAPYIPPPKPAEPPAAEEAPAAEAPAEQPAEEPAADAAAEAPAG"
     gene            complement(883..2391)
                     /gene="ffh"
                     /locus_tag="VFV87_17515"
     CDS             complement(883..2391)
                     /gene="ffh"
                     /locus_tag="VFV87_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5105624.1"
                     /translation="MFESLQTGLRAALKTLQGKARLSEGNMREGLALVEQSLLDADVS
                     YSVAKDFTERVAEKALGEKVLLALNPTQQLVGIIHQELINVLGPVDPAIPLRKDVTVL
                     MMCGLQGSGKTTTCGKLAKLLQQSKKTPMLVAADLQRPAAIEQLHVIGRQLEVPVYSE
                     PGAQDPIAVCTNAIEKARQQQIPVVVLDTAGRLAIDQELMDQLVRIDKKVHPDQVYLV
                     VDGMTGQDAVNSAKAFNEALELDGVIMTKLDGDARGGALLSVKHVTGVPIKFIGTGEH
                     LDALEPFRPEGMAGRILGMGDIVGLFDTVQRSVDLEQQKALEAKLQAGEFTLDDFKNQ
                     LKQVLQPGLMTKMLSLMPGMGQIAEMLNGADAEGEMKRKLGIIDSMTPQERRSPKIIE
                     PGRRNRIARGAGVQVQEVNALLKEYDVMAPLIKSMAGKGIGQRMKALDDLQKSGAFNP
                     GAKAPKIKGDTGKRLTSKERAELKKKREKELRKKKREQKDRDRRQDGDDNMA"
     gene            complement(2582..>3329)
                     /locus_tag="VFV87_17520"
     CDS             complement(2582..>3329)
                     /locus_tag="VFV87_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105625.1"
                     /translation="AASVLESLAQKLPPSERDFSVFEHVTVDGGTTRSAALEFGISQT
                     RVCQILDRVRAWLDEVLPDCDKAATQRQLDLAVALAGDRLDALYGRAVEAWEISAGKI
                     IRTRTTPMGSQVTTTTTSQGDPRYLAAAGKLANLRSKLATSGVLIRGMAAAGDAEEVL
                     ASADYENPPVGQCSGNPAGEAHPAASDADCNVVSPVNEMTSDPITHEEKAARRAFFGP
                     VQELEMSDLAQSALRSKGGPNAAKAARAMA"
BASE COUNT          500 a         1109 c         1030 g          690 t
ORIGIN      
        1 ccggactgct tcgacgcatt cgacgacggg ttccacctgt agaatcatgc cggggccgcc
       61 cccgaagggg cgatcatcga ccttgtggtg tttgtcgcgc gaccagcggc ggatgtcgtg
      121 cagctcgacc tgcaccaggc ccgcggcgat cgctttcttc agcaggctct ggccgaaata
      181 gccctcaaat atcgcaggaa acagggtcag gacgtcgaaa cgcatggacg gtcattggtc
      241 gtagccgcgt tcgccagaac gcgggatttc ggccgattcg cccgcactct ggcgagtgcg
      301 gctactgctc taaccggccg gcgcctcggc agccgcatct gcggccggtt cctccgcggg
      361 ctgctcagcc ggcgcttccg cagcgggggc ctcttccgca gccggcggct cggcgggctt
      421 gggcggcgga atgtacgggg caggggccat cgggcgggtc gttttgatcc gctccagtgc
      481 cacctgctgc tcggccagcc gcgtgccgtc cttgccgtat tttttgatca gcacctggac
      541 attttcggac ggctgcgcgc cgaccgagag ccagtaggcg atccgctcgc cgttcaggat
      601 cgcccgggcg tcggcgtcct tgacgagcgg gtcataatgc ccgagctctt cgatcacctt
      661 gccgtcgcgc ggggcgcgcg aatccatcgc acaaattcgg aagaaggggc gcttgttgcg
      721 gcccagcttc ttcattctca gtcgaactgc cacgggggtc tccttgggtt tggtcagtag
      781 tcagttgggt gagtggtgag tggtgagtgg tgagtggtcg ctggttccca cgctctgcgt
      841 gggaacccac ggccccgacg ctctgcgtcg tctggacgtc gcttacgcca tattgtcgtc
      901 accatcctgc cgccggtcgc gatccttctg ctcgcgtttc ttcttgcgga gctccttctc
      961 gcgcttcttt ttcagctcgg ctcgctcctt cgaggtcaat cgtttgccgg tatccccctt
     1021 gattttcggc gcttttgcgc cggggttgaa agccccgctc ttttgcagat cgtccagcgc
     1081 cttcatccgt tggccgatcc ctttgcccgc catactcttg atcagcggag ccatcacgtc
     1141 gtattccttg agcagcgcgt tgacctcctg cacctgcacg ccagccccgc gggcaatccg
     1201 gttgcggcgg cccggctcga tgatcttcgg actacggcgc tcctgcggcg tcatcgagtc
     1261 gatgatcccc agcttccgct tcatctcgcc ctcggcatcc gcgccgttga gcatctcggc
     1321 gatttgcccc atgccgggca tcaggctgag cattttggtc atcagacccg gctgcagcac
     1381 ctgcttgagc tggttcttga aatcgtcgag cgtgaactcg ccggcttgca gcttcgcttc
     1441 gagcgccttc tgctgctcca gatcgacgga ccgctgcacg gtgtcgaaca ggccgacgat
     1501 gtcgcccatc cccaggattc gccccgccat gccttcgggg cggaacggct ccagggcgtc
     1561 gagatgctcg ccggtgccga tgaatttgat cggcaccccg gtgacgtgct tgacgctcaa
     1621 gagggcgcca ccgcgggcgt cgccgtcgag cttggtcatg atcacgccgt cgagttccag
     1681 cgcctcgtta aacgctttgg cgctgttgac ggcgtcctgg ccggtcatgc catcgaccac
     1741 cagatagacc tggtcgggat ggaccttctt gtcgatccgc acaagctggt ccatcagctc
     1801 ctggtcgatc gccagccggc ctgccgtatc gagcaccacg accggaatct gctgctggcg
     1861 ggccttttcg atggcattgg tgcagacggc gatcgggtcc tgcgcgccgg gctcggaata
     1921 gaccggcact tcgagctgcc ggccgatcac gtgcaactgc tcgatggccg cgggccgctg
     1981 caggtcagcc gcgacgagca tcggcgtctt tttgctctgc tgcaggagct tggcgagctt
     2041 gccgcaggtg gttgtcttgc ccgacccctg caagccgcac atcatcagga cggtcacgtc
     2101 cttgcggagc ggaatcgccg gatcaaccgg ccccaggacg ttgatcagct cctgatggat
     2161 gatgccgacg agctgctgcg tcggattgag ggcgaggaga accttttcgc cgagggcctt
     2221 ctcggccacg cgctccgtga aatccttggc aacgctgtag ctgacgtcgg catcgagcag
     2281 cgattgctcg accagcgcta gcccctcgcg catgttcccc tcgctcagcc gggccttgcc
     2341 ttgcaaggtt ttgagcgccg cgcgcagacc ggtctggagc gattcgaaca tggcgagctt
     2401 gctggtcggc ccggtgtcag gccggagtgt attgccctaa gtggttgatg ccattcaagt
     2461 tgcgctgtga cattagtagc catcccgctc cgcgagatga acgcacatcc agcgcatgcc
     2521 gagccaaacc gtggattcta gcggcaatgt ccgcaacctg taaatggccg gaacggccgc
     2581 gtcacgccat cgcccgtgct gccttcgccg cgtttgggcc gcccttagag cggagagcgc
     2641 tctgggcgag gtcgctcatc tccagctctt gaacaggtcc gaaaaacgct cggcgggctg
     2701 ccttttcctc gtgggtgatt ggatcggaag tcatttcatt gactggacta acgacgttac
     2761 aatctgcgtc agacgccgct ggatgggcct cgccggccgg gtttcctgaa cattgcccaa
     2821 ccggggggtt ttcataatcc gccgatgcca acacttcctc cgcgtccccc gcagccgcca
     2881 tgccgcggat caagacgccg ctggtagcca gcttcgagcg gagattcgcc agcttgcccg
     2941 ccgcggccag ataccgcggg tcgccctgcg aggtggtcgt ggtcgtcacc tggctgccca
     3001 tgggcgtcgt ccgcgtccgt atgattttgc ccgctgaaat ctcccaggcc tccaccgccc
     3061 ggccatacag tgcatccagc cggtcgccgg ccagagccac cgccaggtcg agttgccgct
     3121 gggtcgccgc cttgtcgcag tccggcagca cctcatcgag ccaggcccgc acgcggtcga
     3181 gaatctggca cacccgcgtc tgcgaaattc cgaattccag ggccgcgctc cgcgtcgtcc
     3241 cgccatcgac cgtgacgtgc tcgaagacgg aaaaatcccg ttcgctgggc ggcagctttt
     3301 gagcgagcga ctccagaacc gacgcagca
//
LOCUS       DASUGB010000924         8419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1076199, whole genome shotgun sequence.
ACCESSION   DASUGB010000924 DASUGB010000000
VERSION     DASUGB010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076199"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VFV87_17525"
     CDS             <1..1426
                     /locus_tag="VFV87_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5105626.1"
                     /translation="TRNGEISLLDAKGREIEKYEVPNGAILRVDENQTVKAGQVLCEW
                     DPHSIPILSEVAGKVRYEDVVEGETLRIEKDASGHLRRLIMDHKGELHPQIVLEDAEG
                     KPLDVYYLPERAHIVVEEGKVVSAGTVLGKTPREATGITDITGGLPRVTEIFEARKPK
                     DPAVMAEIDGVVEILQEKRRGKRTIIVKSESGIEREHLVPHGKRFLVHSGDHVKAGQA
                     LVDGPLVPHDILRISGEEALQQYLLHEIQSVYRSQRVDINDKHIEIIVARMLRKVRIE
                     TAGDTSLLPGLVMDRFDFRQINNNLSKCLKIAEKGDSEYADGSIVPKEALEQTNAQIE
                     ALGGTGSKGVKPKSATASTQLLGITKAAVQSNSFISAASFQETTKVLTEAALAGKVDH
                     LVGLKENVILGHLIPAGTGFRQFQESEVRYRPEALQAMQAEKDKVLEAAFPLLQQGDG
                     NGKEGQQPAKPAPASLEKLLEGKE"
     gene            1553..1921
                     /gene="rpsL"
                     /locus_tag="VFV87_17530"
     CDS             1553..1921
                     /gene="rpsL"
                     /locus_tag="VFV87_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5105627.1"
                     /translation="MPTINQLVAKPRRPKRKFSKSPVLEKNPFKQGVCLIVRTMTPKK
                     PNSALRKITRVRLSNGREVTVYIPGEGHNLQEHSIVLVRGGRIRDLPGVRYQVVRGVR
                     DTLGVQNRKQSRSRYGAKKS"
     gene            1970..2440
                     /gene="rpsG"
                     /locus_tag="VFV87_17535"
     CDS             1970..2440
                     /gene="rpsG"
                     /locus_tag="VFV87_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5105628.1"
                     /translation="MGKITASSTSLRPDPKFKSILAGKFINCLMHDGKKSVAQDVFYS
                     ALDAIHEKLPDKDPVEVFTTAVENVKPYIEVRSRRVGGAAYQVPMQVNKNRQQSLAIR
                     WILGACREKKGRPMYLKLSDELLAAYNREGTAYTKRENVHRMADANKAFAHFAW"
     gene            2624..4726
                     /gene="fusA"
                     /locus_tag="VFV87_17540"
     CDS             2624..4726
                     /gene="fusA"
                     /locus_tag="VFV87_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5105629.1"
                     /translation="MSREISKIRNIGIIAHIDAGKTTVTERMLFLSGSKHRVGKVDDG
                     TTETDSDAEEQERGITIYAACVTFPWKTSADTITVNLLDTPGHVDFTAEVERCLRVLD
                     GAVVVFSAREGVEAQSETVWRQADRYKVPRIAFINKLDREGADFESVFEEMRTRLNAN
                     PVAIQIPVGQGPPHVDRPFRGVIDLIEMQLLTFPADRDSRDYTRENVPTDLKSEAEAW
                     RQEMLETLYNYSNELMELALQEQPIPPELIRKVLRETTVHMQVQPVLCGSALHGMGVQ
                     PVLDAVQHYLPSPLDVPPVEGESVGEKQPRKLRRKPSASEPFCGLVFKVLPAKTGDIH
                     WVRVYSGVLKANSRVLNPGKDTKENVAQLWQIHATKVERDGQVESVEAGDIVGIIGLR
                     HSITGDTICDTREPILLETIKFPETVISMAIEPESSTERKKLQDTLEMLKRQDPTLHV
                     QGSETGQTLISGMGELHLEIIKNRLLRDFKLAVKFHKPQVSYRESIARAVEVTGECHR
                     MIAGQQLFAKLTIRMEPAPQSAIPVMLLSAVPPDGLPPDLLTAALEELKGCGEGGGRI
                     AGYPLMKLKVTVLRGEWNPEQTDVRAIKIAAADAFEKGLEQGGKVLLEPIMKLDIVTP
                     EEYLGDFVGDLQQRRAIIAKTENRGKMTAIEAHAPLKELFGYSSAMRSLSQGRAGCSI
                     EPLKYEPAPPEVLKAFDI"
     gene            4878..5201
                     /gene="rpsJ"
                     /locus_tag="VFV87_17545"
     CDS             4878..5201
                     /gene="rpsJ"
                     /locus_tag="VFV87_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5105630.1"
                     /translation="MAAAKEVIRIRMEAYDHAILDQSAQEIVDTAKRTHSEVHGPIPL
                     PTRIERYTVLSGPNIDKKARQQFEIRTHKRLIDIVQATAKTIEALNKLSLPAGVDIKI
                     KASTR"
     gene            5384..6082
                     /gene="rplC"
                     /locus_tag="VFV87_17550"
     CDS             5384..6082
                     /gene="rplC"
                     /locus_tag="VFV87_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5105631.1"
                     /translation="MSKGILGRKVGMTQIYDEAGNCIPVTVVEAGPCRVLQIRTTDRD
                     GYEAIQLGFLDKPRRLARRSERGHVAKLESKRSKKLASAGVQVADKADCEPKRFIREL
                     RGPAGDLKIGQDVTVEALAGVKAVDVTGLTKGRGFSGAMKRHNFGGQRATHGVKKVHR
                     HMGGTGALAANRGGGRMKKGKKMPGQYGHERVTIRNMKLARVDKDNNLLLIHGALPGP
                     NGSYVIVRETNKVG"
     gene            6110..6766
                     /gene="rplD"
                     /locus_tag="VFV87_17555"
     CDS             6110..6766
                     /gene="rplD"
                     /locus_tag="VFV87_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5105632.1"
                     /translation="MVSLTVFNKSGKEVGTYEIDPAQIAPKISQQLLHDVVVMYQANR
                     RQGTHRTKDRGEVAGTTKKMYRQKGTGNARAGSRRAAQRRGGGHVFALRPRDYSYRLP
                     KKAIQAATRMAIARKLQSEDVVVIDELALSEPKTKEIAAILKALGLKDTSTLVATAAL
                     DPVVYKSARNIDKVTISPVAELNALAVLTPRKLLVTKAALDAIKDRAAESNSKKSTSA
                     "
     gene            6797..7114
                     /gene="rplW"
                     /locus_tag="VFV87_17560"
     CDS             6797..7114
                     /gene="rplW"
                     /locus_tag="VFV87_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5105633.1"
                     /translation="MAETTTQPAAEPQLDSYQVILRPLVTEKGMHRSTRHNQYAFEVA
                     TQASKEDIRRAVEDLFNVKVEKIRTQSRRGKPRKHRFKAGQTKAWKKAIVTLNEEHRI
                     DFF"
     gene            7130..7987
                     /gene="rplB"
                     /locus_tag="VFV87_17565"
     CDS             7130..7987
                     /gene="rplB"
                     /locus_tag="VFV87_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5105634.1"
                     /translation="MGIKQYNPVTAGRRGASVSDFAELTKGAAPEKSLLRPLRKKGGR
                     NNQGRITVRHRGGGHKQRYRLVDFKREKDGVPAKVASIQYDPSRNARLALLHFVDGEK
                     RYIIAPEGLKVGQMVENGPDAPPNVGNCLPLSRIPLGVTIHAIEMQPGRGARLCRSAG
                     SNATLMAREADWAQITLPSGEIRRVPAACRATIGQVGNSEAMNVVIGKAGRNRWKGIR
                     PYVRGTAMNPIDHPHGGGEGRTKGGRHPVSPTGVPAKGGSTRQRRKSSNKAIVRRRRS
                     RRYGVLKLK"
     gene            8024..8323
                     /gene="rpsS"
                     /locus_tag="VFV87_17570"
     CDS             8024..8323
                     /gene="rpsS"
                     /locus_tag="VFV87_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX5105635.1"
                     /translation="MSRSSKKGPFVDFKVFKKVQAAQQTGSKEPIKTWARACTIVPEF
                     VGLTFMVHNGKTHVKVFVTEDMVGHRLGEFAPTRTFKGHGGKGKKEAAAAPAPAK"
BASE COUNT         1852 a         2585 c         2654 g         1328 t
ORIGIN      
        1 cacgcggaat ggagaaatct cactgctcga cgccaagggc cgggagattg agaagtatga
       61 ggttcccaac ggcgccattc tccgcgtcga tgagaaccag acggtcaagg cgggtcaggt
      121 tctctgcgaa tgggacccgc acagcattcc gattctgtcg gaggtggccg gcaaggtccg
      181 ttacgaggac gtggttgaag gcgagacgct gcggatcgag aaagacgcca gcggccacct
      241 tcgccgtttg atcatggatc acaaaggcga attgcacccg caaatcgtgc tggaagacgc
      301 cgaaggcaag ccgctcgacg tttattacct gccggaacgg gcccacatcg tggtcgagga
      361 aggcaaggtc gtctcggctg gcacggtgct cggcaagacg cctcgcgagg cgacgggcat
      421 caccgatatc accggcggtc tgccgcgggt gacagagatc ttcgaggccc gcaagccgaa
      481 agatccggct gtgatggccg agatcgacgg ggtcgtcgaa atcctgcagg aaaagcgccg
      541 cggcaagcgg acgattatcg tcaagagcga aagcggcatc gagcgcgagc acctggtgcc
      601 gcacggcaag cggttcctcg tccactcggg cgaccacgtc aaggcggggc aggccctggt
      661 cgatggtccc ctggtgccgc acgacatcct gcgaatttcg ggcgaagagg ccctgcagca
      721 gtacctgctg cacgaaatcc agagtgtgta tcgttcgcag cgcgtcgata tcaacgacaa
      781 gcacatcgag atcatcgtcg cccgcatgct ccgtaaagtg cgcatcgaaa cggcgggcga
      841 tacgagcctc ctgccggggc tggtcatgga ccgcttcgac ttccggcaga tcaacaacaa
      901 cctgagcaag tgcctcaaga tcgccgagaa aggggacagc gaatacgccg acggctcgat
      961 cgttcccaaa gaggccctgg agcagacgaa tgcccagatc gaagccctcg gcggcacggg
     1021 atcgaaggga gtcaaaccga agtccgctac ggccagcacg cagcttttgg gcatcaccaa
     1081 ggcggccgtg cagtccaaca gcttcatctc ggcggccagc ttccaggaaa cgaccaaggt
     1141 gctcaccgag gcggccctgg ccggcaaggt cgatcatctg gtcggcctga aagagaacgt
     1201 gatcctcggc cacctgattc ccgcgggcac cggcttccgc cagttccagg aatcggaagt
     1261 ccgctaccgc ccagaagcct tgcaggccat gcaggccgag aaggacaagg tgctcgaagc
     1321 cgcctttccg ctcttgcagc agggcgatgg gaacggcaag gaaggtcagc agcctgccaa
     1381 gcccgccccg gcttcgctgg agaagctgct ggaaggcaag gaatagaagg actgttctta
     1441 gccgggacca aacctggtcc cggtgcgcgt ggaaacgagc gactgccttg acggtcgccc
     1501 gcattccgct aagctactgg attccctcca ccggaatcga atcgttcact gcatgcctac
     1561 gatcaatcaa ctcgttgcca agccgcggcg gccgaaacgc aagttcagca agtcgccggt
     1621 cctggagaaa aaccccttca agcagggggt ctgcctcatc gtccgtacga tgacccccaa
     1681 gaagccgaac tcggcattgc ggaagatcac gcgcgtgcgt ctttccaacg ggcgcgaagt
     1741 gacggtgtac attcccggcg aagggcacaa tctgcaggaa cactcgatcg tgctggttcg
     1801 cggcggccgt atccgcgatc tgccgggtgt gcgttatcag gtcgtccgcg gcgtccgcga
     1861 cacgctgggc gtgcagaacc gcaaacagtc ccgcagccgc tacggcgcga agaagtcata
     1921 gtcaccagtg cggtgcgagc atcgcagcga attagaagac atctgcaaca tgggcaagat
     1981 aaccgccagt tcaacttctc tgcggcccga tcccaagttc aaatcgatct tggccggcaa
     2041 gttcatcaat tgcctgatgc acgacggcaa gaagagcgtg gcccaggacg tgttttactc
     2101 cgccctggac gccatccacg agaagctgcc cgataaggac ccggtggagg tcttcaccac
     2161 ggcggtcgag aacgtcaagc cgtacatcga ggtccgttcc cgtcgcgtcg gcggcgctgc
     2221 ctaccaggtg ccgatgcagg tgaacaagaa ccggcagcag tcgctggcga ttcgctggat
     2281 tttgggcgcc tgccgcgaaa agaagggccg cccgatgtat ctcaagctct ccgacgagct
     2341 cctggccgcg tacaatcgcg aaggcaccgc ctataccaag cgcgaaaacg tgcatcgtat
     2401 ggccgatgcc aacaaggctt tcgctcactt cgcctggtag ctggaaccaa tcatgggccg
     2461 cggtgcgagg agcatcgcgg ctttttcatg cgcggaggaa ggggttcggg actcggggtt
     2521 cgggactggg gagactgacg atgaatgaag acgacgaccc gccttagcca ccgccaaagt
     2581 cgcacccgag ccccgagtcc ccagccccga accccttgct cccatgtccc gcgaaatctc
     2641 gaaaatccgg aacatcggca tcattgccca catcgacgcc ggcaagacga cggtaaccga
     2701 gcggatgctc tttctcagcg ggtcgaagca ccgcgtgggg aaggtggacg acggcacgac
     2761 cgagaccgac agcgacgcgg aggagcagga gcgcggaatc accatctacg ccgcgtgcgt
     2821 cacgtttccg tggaagacca gtgccgacac catcacggtc aacctgctcg atacgcccgg
     2881 gcacgtcgat ttcaccgccg aagtcgagcg ctgtctgcgc gtgctagatg gcgccgtggt
     2941 tgtgtttagc gcccgcgagg gcgtcgaagc gcagagcgag accgtctggc ggcaagcgga
     3001 ccgctacaag gtgccgcgca tcgcgttcat caacaagctg gaccgggaag gcgccgattt
     3061 tgaatcggtc tttgaggaaa tgcggacgcg gctgaatgct aacccggtgg ccattcagat
     3121 tccggttggc caggggccgc cgcacgtcga tcggccgttc cggggcgtga tcgacctcat
     3181 cgaaatgcaa ttgctgacgt ttccggccga cagggacagc cgtgactata cgcgcgagaa
     3241 tgtgccgacc gacctgaagt cggaagccga agcctggcgg caggagatgc tcgaaacgct
     3301 gtacaactac agcaacgagt tgatggagtt ggccctgcag gagcagccaa tcccgccgga
     3361 gctgatccgc aaggtcctgc gcgaaacgac agtccacatg caggtccagc cggtcttgtg
     3421 cggttcggcc ttgcacggca tgggcgtgca gcccgtgctc gacgcggtgc agcactatct
     3481 gcccagcccg ctggacgttc cgccggtcga aggggaaagc gtcggcgaga aacagccgcg
     3541 aaagctccgc cgcaagccga gcgcgtcgga gccgttctgc gggctcgtgt tcaaagtcct
     3601 gccggccaaa acgggcgata tccactgggt ccgcgtctat tccggcgtgc tcaaggcgaa
     3661 cagccgcgtg ctcaaccccg gcaaggacac gaaggaaaac gtcgcccagc tttggcaaat
     3721 tcatgccacc aaggtggagc gcgacggcca ggtcgaaagc gtcgaggctg gcgacattgt
     3781 cggcatcatc ggcctgcggc actcgatcac cggcgacacg atctgcgata cgcgcgagcc
     3841 gattttgctg gaaacgatca agtttcccga aacggtcatt tcgatggcga tcgagcccga
     3901 gtcgagcacc gagcggaaaa agctgcagga cacgctggag atgctcaaac ggcaggaccc
     3961 gacgctgcac gtccagggga gcgaaacggg gcagactcta attagcggca tgggcgaatt
     4021 gcacctggaa atcatcaaga atcgcttgct gcgggatttc aagctggcgg tcaaattcca
     4081 caagccgcag gtcagctatc gcgagtcgat cgccagggcg gtggaagtaa cgggcgaatg
     4141 ccaccggatg atcgccggcc agcagctttt cgcgaaactt acgatccgca tggagcccgc
     4201 gccgcagagc gcgattcccg tgatgctgct cagcgccgtt ccgcccgacg gcttgccgcc
     4261 ggatttgctc acggcagccc tggaggagct caaaggctgc ggcgaagggg gcggccggat
     4321 cgcgggctat ccgctgatga agctcaaggt gacggtgctt cgcggcgagt ggaatccgga
     4381 gcaaaccgac gtgcgggcga tcaagatcgc cgcggccgac gccttcgaaa aaggcctgga
     4441 gcaaggcggc aaggtgctgc tggagccgat tatgaagctc gacatcgtca cgcccgaaga
     4501 gtatctgggc gatttcgtgg gcgacctcca gcagcggcgg gcgatcatcg ccaagaccga
     4561 gaaccgcgga aaaatgaccg ccatcgaagc ccacgccccc ctgaaggaac tgttcggcta
     4621 ttccagcgcg atgcggagcc tcagccaggg ccgcgctggc tgttcgatcg agccgctcaa
     4681 gtacgaaccg gcgccgccgg aagtcttgaa ggcgtttgac atctgacacg ccgctccacg
     4741 taacttacga tccgaggcgt tgacggtcct aatcgcctcc agtaggattt ggagtttcca
     4801 atcttcggat tgggattcac tggaaacgag attcccgcac cgattccaac gcgaatcgct
     4861 gctgtggagc agatatcgtg gctgctgcga aagaagtcat cagaatccgg atggaggctt
     4921 atgaccacgc aattctggac cagagcgccc aggaaatcgt ggacaccgcc aagcggactc
     4981 atagcgaagt gcatgggccg attccgcttc ccacgcggat cgaacggtac accgtgctct
     5041 ccggtccaaa catcgacaag aaagcgcgcc aacagttcga gattagaacc cacaagcgcc
     5101 tgatcgacat cgtgcaggcg acggcaaaga cgattgaagc gctgaacaag ctcagcttgc
     5161 cggcgggtgt ggatatcaaa atcaaagcgt caacgaggta agtgaatggt tggtatctga
     5221 atggtgagtg gttggtggtg agtggtgttc gcgtcaaaca ctactcacca cttaccactc
     5281 actgggttcg accaaaggtc acctatcgac tggaagccac gcggggaatg cgaaagcgtg
     5341 ctttcgccat cccgtcgggc gactgaccac aaggtacatc gcgatgagca agggaatact
     5401 cggccgcaag gtcgggatga cgcagattta cgacgaagcc gggaattgca tcccggtcac
     5461 cgtcgtcgaa gccggtcctt gccgggtgct gcagattcgc accaccgacc gcgatggtta
     5521 cgaggcgatc cagctcggct ttctcgataa gccgcggcgt ctggctcgcc ggagcgaacg
     5581 tggtcatgtc gcgaagctgg agagcaagcg gtcaaagaag cttgcctccg ccggcgtgca
     5641 ggttgccgac aaggcagatt gtgagccgaa gcgctttatt cgcgagctgc gcggacctgc
     5701 gggcgacctg aagatcggcc aggacgtgac ggtcgaggct ttggcagggg tcaaggcggt
     5761 cgatgtcacg ggactgacca agggccgcgg gttttccggc gcgatgaagc gtcacaactt
     5821 tggcggccag cgggcgacgc acggcgttaa gaaggtgcat cgccacatgg gcggcaccgg
     5881 cgcactggca gccaaccgcg gcggcggccg catgaagaag ggcaagaaaa tgcccggcca
     5941 atacggccac gaacgagtga cgatccgcaa catgaaattg gcccgggtgg acaaggacaa
     6001 caatttgctt ctcattcacg gcgccctgcc cggtcccaac ggcagctacg tgatcgtccg
     6061 cgaaacgaat aaggtgggat aggcgtgagc cttccgatgg aaatgagtca tggttagcct
     6121 cacggttttc aacaagagcg gcaaagaggt cggcacctac gagatcgacc cggctcagat
     6181 cgcgcccaag atcagccagc agcttctgca cgacgtggtc gtgatgtatc aggcgaatcg
     6241 ccgccagggg acgcatcgca cgaaggaccg cggcgaagtc gccggcacga ccaagaaaat
     6301 gtaccgccag aagggcacgg gcaacgcccg cgccggctcg cggcgggccg cgcagcgccg
     6361 gggtggcggc catgtctttg cgcttcgccc gcgggactac agctatcggc taccgaaaaa
     6421 ggccatccag gccgcaacgc ggatggcgat tgcccgcaag ctgcagtccg aggatgtggt
     6481 cgtgatcgac gagttggccc tgagcgagcc caagaccaag gaaatcgctg cgattctcaa
     6541 ggcgctcggc ctcaaggaca caagcaccct cgtggcgacg gctgctctcg atccggtcgt
     6601 gtataagagc gcccgcaaca tcgacaaagt cacgatttcg cccgtcgccg agctcaacgc
     6661 tctggcggtg ctcacgccgc gcaagctgct cgtcaccaaa gccgccctcg acgcgattaa
     6721 agaccgggcg gccgaatcga actccaagaa atcaactagc gcctgaccgc tgagcttcgc
     6781 gctcagtaaa catcgcatgg ccgaaacgac cacacaaccc gcagccgagc cgcagctcga
     6841 ttcgtaccag gtcatcctgc gaccgctggt gacggaaaag ggtatgcacc gctcgacgcg
     6901 gcacaaccag tacgcattcg aagtggcgac ccaggcgagc aaggaagaca tccgccgggc
     6961 ggtcgaggat ctgttcaatg tgaaggtgga aaagattcgc acccaaagcc gccgcggcaa
     7021 gccgcgaaag caccgcttca aggcgggcca aacgaaggcc tggaagaaag cgatcgtgac
     7081 tctcaacgag gaacatcgga tcgacttctt ctagtcaaga gctgatacca tgggaatcaa
     7141 gcaatacaac cccgtcacag ccggccgccg cggcgcgagc gtcagcgatt ttgccgagct
     7201 gaccaaaggc gcagcgccgg agaagtcgct cctgcgaccg ctgcgcaaga agggcggacg
     7261 gaacaatcag ggtcgcatca ccgtccgcca tcgcggcggc ggccacaagc agcgctatcg
     7321 cctggtcgat ttcaagcgcg agaaggacgg cgtgcccgcc aaggtcgctt cgattcaata
     7381 cgatccgagc cgcaatgcac gcctcgcgct gctccacttc gtggacggcg agaagcgtta
     7441 catcatcgct ccggaggggc tcaaggtcgg acaaatggtc gagaacggcc cggacgcccc
     7501 gccaaacgtc ggcaactgcc tgccgcttag ccgcattccg ctgggcgtca cgatccacgc
     7561 cattgaaatg cagcccggcc ggggcgctcg actgtgccgc tcggccggct caaatgcgac
     7621 gctgatggcc cgcgaggccg attgggctca gatcacgctt cccagcggcg agattcgccg
     7681 cgtgccggct gcgtgccggg cgacaatcgg ccaggtcggc aacagcgagg cgatgaacgt
     7741 cgtgatcggc aaggccggcc gcaaccgttg gaaggggatc cggccatacg tccgcggcac
     7801 cgcgatgaac ccgattgatc acccgcacgg cggcggcgaa ggccgcacga agggcggtcg
     7861 ccatccggtc agccccacgg gcgtgcccgc caaaggcggc tcgacccgcc agcgccgcaa
     7921 gtcctcgaac aaagccatcg tccgccgccg ccgcagccgg cggtatggag tgttgaaact
     7981 gaaataactg tagggaacgc cctccgtggc gttccgaagc cgcatgagcc gttccagcaa
     8041 aaaaggtccg tttgtcgatt tcaaggtctt caagaaggtc caggctgcgc agcagaccgg
     8101 gtcgaaggag ccgatcaaga cttgggcccg cgcctgcacc atcgtgcccg agttcgtcgg
     8161 cctcacgttc atggttcaca acggcaagac gcacgtgaaa gtgttcgtga ccgaagacat
     8221 ggtcggccat cggctgggtg agttcgcccc gacccgcacg ttcaaaggcc acggcggcaa
     8281 aggcaagaag gaagccgcgg cagcgcccgc ccccgccaag taaccgcagg tacatcatgc
     8341 cctatcgagc ctcccatcgc ttcgcccgga tcagcgcccg caaggtgcgc ccgatcgccg
     8401 atatgattcg cggcaaatt
//
LOCUS       DASUGB010000925         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1771242, whole genome shotgun sequence.
ACCESSION   DASUGB010000925 DASUGB010000000
VERSION     DASUGB010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771242"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VFV87_17575"
     CDS             complement(<1..1158)
                     /locus_tag="VFV87_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5105636.1"
                     /translation="MGGYRSLKRVLGESNLERKFRWLFGLSIGTLIFAAFLGVWYVAK
                     NLIEDTAQHKGSDLVRVVLFDLHWKRWNAGDVAQEKLRQQMSDALSQEQFSSAILKLD
                     NTPEPMLTGTNVIVPSTEYERNLVADLKRRAESEFASTVAPESSSPAGADVETILPTI
                     ATARVSPDRRAYYYYQVVHWSDRCMSCHDGMHGRFAESAATFDGGVFDPLSKPFRVVR
                     VTMPYDETRTAINFTIAVLITVGFLTVFLSMVAFYFVVKYIVIKPLTHLRDVSDEVAS
                     GDLEQRAEIHTGDEFEELAESFNKMLRHLIETQAELRGANDELDQKVDELARLNMQLH
                     EMNRMRGEFLANMSHELRTPLNSIIGFAEVLQGLDSLSEKQKRYAQNIQKSG"
     gene            1347..2438
                     /locus_tag="VFV87_17580"
     CDS             1347..2438
                     /locus_tag="VFV87_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105637.1"
                     /translation="MIPNRRDVLAAGAALAVPALLPRWICGEETPQPESKTPGAETAS
                     FKEFTPQSERAVARGDEWLMKTMHRDGGCGVDIGQPTDIGCTAMVGLALMAQGNTPVE
                     GPKSREVQRILSFILRTTENMPTDDITPQVGTQLQNKIGRHAHSFFAATFLAEVIGEG
                     WDTELVREGLKRVVTAIVGSQTQQGHWGDQSWAPMLGTVMGWVSLRSAHYAGIKVGGS
                     PELVASYIEQQMQMNLAQQQGSWMHTLYKNATGIRVLYALGKEDDPIAKKAFVDVKQL
                     VNKDNTAFSQAGGEEYLAFHLITETMLQKGGDDWATWFPTLRDKIVGVQNSDGSWTGH
                     HCITSRTFCTAAAILVLTAPNRYLPISQA"
     gene            2526..3635
                     /locus_tag="VFV87_17585"
     CDS             2526..3635
                     /locus_tag="VFV87_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5105638.1"
                     /translation="MKAEIEKSLTRGCEWLIVQQADDGGWHSKTYGQLKDGAAVTALA
                     LHTLSLCSKEIRQQHAGAIERGFAFLDSGIEKRGTLASPDGSLDFPTYAAALWLSAVN
                     RLWHPRDSRKVVEYLVGAQVVEARGFDKSSPSYGGWDFLGPDDAKGITTGTNISVTRH
                     ALEALAIQRDRNQKGGLSREATEALKDPIQGTGKAYVLRCQQADGGFAFTCEPASLNN
                     KAAYSESELTKPRSYGTATCDGVLALVACIKATAPGGLPDFDKPVTIEDPAITKALAW
                     LAKRPGLELVPGFEGFPPEAGWDRGLRFYYYAALSRVLPLFPLSERDSRSAALARHII
                     GLQQEDGRWENESDRMRENDPLIATALAVVALANH"
BASE COUNT          810 a         1153 c         1164 g          691 t
ORIGIN      
        1 gccggatttc tggatgttct gggcgtaccg cttctgcttc tcggaaagcg agtcgagtcc
       61 ctgcagcacc tcggcaaagc cgatgatgct attgaggggc gtgcggagct cgtggctcat
      121 gttggcaagg aattcaccgc gcatgcggtt catttcatgg agctgcatgt tcaagcgggc
      181 caattcatca accttctgat ccagctcgtc attggccccg cgcagctccg cctgcgtttc
      241 aatcaggtgc cgcagcatct tgttgaacga ctcggccagc tcctcaaact cgtcgcccgt
      301 atggatctcg gcccgctgct ccagatcgcc gctcgcgact tcgtcgctca cgtcgcgcaa
      361 gtgcgtgagc ggcttgatga cgatgtattt cacgacgaag tagaaggcca ccatcgagag
      421 gaacaccgtc aggaagccca cggtgatgag cacggcaatc gtgaaattga tggcagtccg
      481 cgtttcgtcg taaggcatgg tcacgcgaac gacgcggaag ggcttactca gcggatcgaa
      541 cacacctcca tcaaatgtcg cggccgactc ggcgaagcgg ccgtgcattc cgtcatggca
      601 gctcatacag cgatcggacc aatgaacgac ctgatagtag tagtaggctc ggcgatcggg
      661 gctcacacgg gccgtagcga tggtgggaag aatggtctcc acgtccgctc cagcaggact
      721 ggagctttcc ggagccacag tactggcgaa ttcagattcg gctcgacgct tcagatcggc
      781 aaccagattc cgctcatact cggtcgaggg gacgattaca ttcgtgcccg tgagcattgg
      841 ctcgggtgta ttgtcgagct tgagtattgc cgacgagaat tgctcctgag agagcgcatc
      901 ggacatttgc tggcgaagct tctcctgggc cacgtctcca gcattccacc gcttccaatg
      961 cagatcgaac agaacgacac gcaccagatc gcttcccttg tgctgcgccg tatcttcgat
     1021 caggttcttc gcgacatacc aaacccccag aaacgccgcg aagatcagcg tcccgatgct
     1081 gaggccgaag agccagcgga acttgcgttc cagattgctc tcgccgagca cgcgtttgag
     1141 ggatcgatag ccgcccatcg caccaggttt gcccagagac cgctaagtgt gcaaattcat
     1201 tgtagggacc gtggaaacag gccactaggc caatcgcccg catgttgatt gacgcctggg
     1261 gcttccgcac ttcacaatac aagactggcc gtgccgcgaa gagtggcgct ctagcggaga
     1321 ggttacggca gagagaagga gaagcgatga ttcccaaccg acgcgacgtg ctggcggcag
     1381 gcgccgctct ggcggtgccg gcattgctgc cgcgctggat atgtggcgag gagacgcccc
     1441 agcccgagtc caagacgcct ggcgcagaga cggcgtcgtt caaggaattc acgccgcaga
     1501 gcgagcgggc cgtggcccgc ggcgacgagt ggctgatgaa gaccatgcac cgcgatggcg
     1561 gctgcggcgt cgatatcggt cagccgaccg acatcggctg cacggcgatg gtcggcctgg
     1621 ccctgatggc ccaggggaac acgccggtcg aaggccccaa aagccgcgag gtgcagcgaa
     1681 ttctgtcgtt tatcctgcgg accacggaga acatgccgac cgacgacatc acgccgcagg
     1741 tcggcacgca gttgcagaac aagatcggcc gccacgccca cagtttcttc gccgccacgt
     1801 tcctggcgga agtcatcggc gaaggctggg acacggaact cgtccgtgag gggctgaagc
     1861 gcgttgtcac ggcgatcgtc ggttcgcaaa cgcaacaggg acactggggc gatcaatcgt
     1921 gggcgccgat gctgggcacc gtgatgggct gggtctcgct acggtcagcg cactatgcgg
     1981 ggatcaaggt gggcggctcg ccggagctcg ttgcgagcta catcgagcag cagatgcaaa
     2041 tgaatctcgc ccagcaacag ggaagctgga tgcacacgct ctataagaac gccaccggta
     2101 ttcgcgtact gtatgctctg ggtaaagaag acgacccgat cgcaaaaaag gcgtttgtcg
     2161 atgtcaaaca actcgtgaac aaggacaaca cggcgttctc gcaagcgggg ggtgaggagt
     2221 atctggcgtt ccacctgatc actgaaacga tgctgcaaaa ggggggtgac gactgggcca
     2281 cgtggttccc gacgctccgc gacaagatcg tcggcgtgca gaactccgac ggctcatgga
     2341 ccggccacca ttgcatcacc agccgtacgt tctgcaccgc cgcagcgatc ctggtgctga
     2401 cggccccgaa ccggtatttg ccaatctcgc aggcgtaagg tcgagttgat tcgtttaacc
     2461 cggagccgca ggcgacgctg caccgtcggg caagcgaaga accgcggatc acgcggagac
     2521 cgcggatgaa ggcagaaatc gaaaaatccc tcacccgcgg ctgcgaatgg ctgatcgtcc
     2581 agcaagccga cgacggcggc tggcattcga agacctatgg gcagctcaag gacggagcag
     2641 ccgtcacggc cctggcacta cacaccctta gtttgtgctc caaagaaatt cgccagcagc
     2701 atgcgggagc aatcgagcgc ggattcgcct tcttggacag cggtattgaa aaacggggaa
     2761 ctttggccag tccagatggt tcgcttgact tcccgaccta tgccgcggct ctgtggctgt
     2821 cagccgttaa tcggctgtgg cacccgcgag acagtcggaa agtcgttgaa tacctggtag
     2881 gcgcccaggt tgtggaggcg cgcggtttcg acaagagttc gccaagttac ggcggatggg
     2941 attttctagg gccagatgac gcgaagggca tcacgaccgg aaccaatatt tcagtgacgc
     3001 gccacgctct ggaagcgctg gcaatacaac gcgatcgtaa tcaaaagggc gggttgtcgc
     3061 gcgaagcgac cgaggcactt aaggacccga tccaaggcac agggaaagcc tacgtcctcc
     3121 gctgccaaca ggccgatggc gggttcgcat tcacctgcga accggcgtcg ctcaacaaca
     3181 aagccgcata ttccgaatcg gagctgacca aaccccgctc ctacggcacg gcaacgtgcg
     3241 acggagtcct ggcgctggtt gcttgcatca aagccactgc tccaggcggc cttcccgatt
     3301 ttgacaagcc cgtcaccatc gaggatccag cgattaccaa ggccctcgcc tggctcgcca
     3361 agcggccggg cctcgaactc gttcccggct ttgaaggctt tccgcccgag gccggctggg
     3421 accgcggctt gcggttctac tactacgccg cactcagccg ggtcctgccc ctcttcccac
     3481 tctccgaacg cgattcccgt tcggccgcgc tcgccaggca catcattggc ctgcaacagg
     3541 aagacggccg ctgggagaac gaatcggacc ggatgcgcga aaacgacccg ctgatcgcca
     3601 cggccctggc ggttgtggcg ctagcgaatc actgacggag tgaccggctc aatccagatc
     3661 aaaatgccct cgttgggatt gggcgcagga gatgctacta cccctccggg acctccccct
     3721 atccatgaag tttggatgga gcgcctctgc ttgagcagaa tcaccggtcg atcgcccggg
     3781 aaagattcgc ttctcgcgaa atgcacacca ttggaacc
//
LOCUS       DASUGB010000926         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1353880, whole genome shotgun sequence.
ACCESSION   DASUGB010000926 DASUGB010000000
VERSION     DASUGB010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353880"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFV87_17590"
     CDS             <1..565
                     /locus_tag="VFV87_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105639.1"
                     /translation="KARDAKIEEQAKTIAGLAEKLTELEAKLARVSDESSVVNQPQGS
                     ANPACISQSLMHPTPGVSSRVHGAERNARMSDASSVVNQSQGSANPACISQSLMHPTP
                     AAPATLDESRIAQTTPAQNQTSPDSQVAKPIAEAPSGDALDLPPAVSQRPESDESRPA
                     SPTRKPPTWVTVLGPGGRLIWLKKPEK"
     gene            946..1950
                     /locus_tag="VFV87_17595"
     CDS             946..1950
                     /locus_tag="VFV87_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5105640.1"
                     /translation="MTSRPSAVLVCQPLLFNKPGAYRAILESAGMEVRFPRTGGTVLS
                     EAQLAAELDGVTATIASTEPYTVAILQTAPGLRVISRTGVGYDSIDVGAATERGIVVA
                     CTPGTNHDAVAEHTFALLLAVAKRVVPYHAEVAQGGFRRKPSAALRGKTLGLVGLGRI
                     GRAVARRAAAFDMRVLAHDPFLTAVLPDARGVELVPFDELLAQSDFISLHAAATDGTS
                     RLICRESLAQMKQGVVILNTARGTLIDEGALAEALQSGKVAAAGIDVFDREPPADSPL
                     LSAPNVVLSPHIAGIDEQAFEDMVTMAAQTIADLYQRKWPAERLVNGSQLPQPWKWPA
                     "
     gene            complement(1958..>2650)
                     /locus_tag="VFV87_17600"
     CDS             complement(1958..>2650)
                     /locus_tag="VFV87_17600"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5105641.1"
                     /translation="VWIDLEHNAMTTESMLGHLMAARAGGCASIVRIPSGDVAWIKRV
                     LDSGAEGIILPRAYSAKEVEAFVSACRYPPLGTRGFGPRRPMQYGRLEQQAYLNQANR
                     DVFVVAQVETVELLAQLDEVLKLEGLDSLVLGPQDLSGSMGRLGETTHPEVVAAMKTV
                     ATRARAAGKFIGSGLGANPKFAKVLIDCGVQWMQAGNDFEYMIRSCERTFAEIRGDAP
                     GSSAVDSYGQKL"
BASE COUNT          502 a          827 c          864 g          457 t
ORIGIN      
        1 gaaagcccgc gacgcgaaaa tcgaggagca agccaagacg atcgcggggc tggcggaaaa
       61 gctgacggag ctggaagcca agctggcacg cgtgagcgac gaatcgtcgg tggtgaatca
      121 accccagggg agcgcgaatc ccgcgtgcat ttcgcaaagc ctcatgcacc ctacgccggg
      181 cgttagcagt agggtgcatg gagcggagcg gaatgcacgc atgagcgacg catcgtcggt
      241 ggtgaatcaa tcccagggca gcgcgaatcc cgcgtgcatt tcgcaaagcc tcatgcaccc
      301 tacgccggct gcgccggcaa ctttggatga atcaagaatc gcccaaacga cccccgctca
      361 gaatcaaacg tcgcccgatt cacaagtcgc aaagccaatt gccgaggcac cttccggcga
      421 cgcgcttgat ttgccgccgg ccgtttcgca gaggcctgaa tccgatgaat cacggcccgc
      481 cagcccgacc cgcaaaccgc cgacgtgggt caccgttctg ggtcccggcg ggcggctcat
      541 ctggctgaag aaacctgaaa agtgaaatct gaaaactgcc ctccaccgga gaatccctca
      601 tgtcgactcc ttccgcttgt tccgagcaat tccttcgtcc cgatctggcg tcagccggaa
      661 cgacgctggc cgaaaaccat ctgcatgaat cacactactc gccgcacact ccttccacga
      721 ggcagcagca gtttctcgac ctggactgcc tggaggcttt ctatggcggg gcggccggcg
      781 gcgggaagag cgaggcgctt ggcgcccggg aaggtacggg tgtaacgcgc gtccgttatg
      841 atgatagcgt gcgttttggt cggcggtcct tatcctggga gcatcagcgc ccgctgtcaa
      901 gaacgaagct gctgcggcag ccaaatagaa ccgccggagc catccatgac cagtcgaccg
      961 tccgccgtgc tggtctgcca gcccttgctc ttcaacaagc ccggagctta tcgcgcgatt
     1021 ctcgaatcgg ctggaatgga ggttcggttt ccgcggacgg gaggcactgt gctgagtgag
     1081 gcacaactgg cggccgagct cgacggagtc acggcgacga tcgccagcac cgagccgtat
     1141 acggtggcaa tcctgcagac ggctccaggg ctgcgggtga tttcccggac gggggtcggc
     1201 tacgacagca tcgacgtggg ggcggcgacg gagcggggca ttgtggtggc ttgcactccc
     1261 ggcacgaatc acgacgcggt tgccgagcac acgtttgcct tgctgctcgc ggtggcgaag
     1321 cgagtcgtcc cctatcacgc cgaagtcgcc cagggaggat ttcgccgcaa gccatcggcg
     1381 gctctgcggg ggaagacgct gggactggtc gggctgggcc ggattggccg ggcggtcgct
     1441 cgccgcgcgg cggccttcga catgcgggtc ctggcgcacg atccgttttt gacagcggtg
     1501 cttccggacg cacgcggagt cgaactggtc cccttcgacg aattactcgc ccaatccgat
     1561 ttcattagcc tgcatgccgc ggcaaccgac ggaacgtcgc gactgatttg ccgggagagt
     1621 ctggcccaga tgaaacaggg ggtggtgatt ctcaataccg cgcggggaac gctcatcgac
     1681 gagggggcgc tggcggaagc cttgcagtcg ggaaaagtcg cggcggcggg gatcgacgtt
     1741 tttgatcgcg aaccgccggc ggactcgccc ctcctatccg cgcccaacgt cgtgctttcc
     1801 cctcatatcg ccgggatcga cgagcaggct ttcgaggaca tggtaacgat ggcggcgcag
     1861 acgattgccg atctgtatca gcggaagtgg ccggcggagc ggctcgtcaa tgggagccaa
     1921 ttgccacagc cgtggaagtg gccggcgtag gcggccgcta cagcttctgg ccgtaggagt
     1981 cgactgccga cgagccggga gcgtcgccgc ggatttcagc gaaggtccgc tcgcaactcc
     2041 ggatcatgta ttcaaaatcg ttgccggctt gcatccattg cacgccgcag tcgatcagga
     2101 ccttggcgaa ctttggatta gcacccaggc cagagccaat gaactttcca gccgccctgg
     2161 ctctcgtggc gacggttttc atggccgcga caacctcggg gtgagtcgtc tctccgagcc
     2221 gtcccatcga acccgacaaa tcctgcggcc cgagaacgag ggagtcgagt ccctcgagct
     2281 tcaggacttc atcgagctgg gccaggagct ccacggtctc aacctgggcc acgacgaaca
     2341 cgtcgcggtt cgcctggttc agataggcct gctgctcgag ccggccatat tgcattgggc
     2401 ggcgagggcc gaacccgcgg gtgccgagcg gcggatagcg acacgccgag acgaatgcct
     2461 cgacctcttt cgccgagtag gcccgcggca ggatgattcc ttccgcgccg gaatcgagca
     2521 ctcgcttgat ccaggccacg tcgccggacg ggatgcggac gatggaagcg cagccgccgg
     2581 cgcgtgccgc catcaagtgg cccagcatcg actcggtggt catggcgttg tgctccaggt
     2641 cgatccagac
//
LOCUS       DASUGB010000927         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1633572, whole genome shotgun sequence.
ACCESSION   DASUGB010000927 DASUGB010000000
VERSION     DASUGB010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633572"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFV87_17605"
     CDS             complement(<1..577)
                     /locus_tag="VFV87_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105642.1"
                     /translation="MNVFRSSQFALGLIIAAALALAGCAAGRGARAQRSDGSAPPVPA
                     IYGREESRKSADGLAARTVVPPAIAASQRMPPPPQDAPPGAVQPAAFHAALVEPAVDL
                     VQPPARPPAQQPAATPAEPLPIAPAAPTAERLPAERPTVPIDLATALALTHGQNPRIA
                     FAQAQIAQSLAQYDAARVLWLPSLRAGMNYNK"
     gene            complement(1012..1605)
                     /locus_tag="VFV87_17610"
     CDS             complement(1012..1605)
                     /locus_tag="VFV87_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105643.1"
                     /translation="MNRSAAFFILAAFLAGGLLGVLLCAASSDLGTAGSAAYGQQPAN
                     APPVALATLAQEVERLKRVVPDQSHAMSDVDYHFSNLWYAGKARNWPLADFYWKESLA
                     HMKWAVRIIPVRKDNAGKEVKLEDILKSIENSPYMQMGEVIKRQDAEKFEPTYKYIVE
                     GCYSCHKASDKPYLRPHIPERPASAMINFKPQADWPQ"
     gene            complement(1727..>2203)
                     /locus_tag="VFV87_17615"
     CDS             complement(1727..>2203)
                     /locus_tag="VFV87_17615"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5105644.1"
                     /translation="MLRGTGKAGDRLILLRRERQSISRFDIMANNVLKHLFTGFVRLH
                     ILYHADKQPVCGVELMEELQHHGYSIGPGTLYPVLHGLEEAGFVKAKDEVVGGKRRKN
                     FRTTARGRKLLVEARGKLIELASEIVDDQDEMTERRKHSQKHAEIAGEGELSRSAG"
BASE COUNT          401 a          699 c          689 g          414 t
ORIGIN      
        1 gcttgttgta gttcatcccg gcccgcaggc tcggcagcca cagcacgcgg gccgcgtcgt
       61 actgggccag cgactgggca atctgagcct gggcaaatgc gatccgcggg ttctggccat
      121 gcgtcagggc cagggcggtt gcgaggtcaa tcggcaccgt gggccgctca gccggaagcc
      181 gctcagccgt gggtgccgcc ggtgcaatgg gcagcggttc ggccggagtt gcggcgggct
      241 gctgagcggg cggccgagcg ggcggctgaa ccagatcaac ggcgggctcg accagcgccg
      301 catgaaacgc ggcgggctgg acggcgcccg gcggcgcgtc ctgcggcggc ggcggcatgc
      361 gctggctggc tgcgatcgcc ggcgggacga cggtccgcgc ggccagccca tcggcactct
      421 tccggctttc ttcacggccg tagattgcag gcaccggcgg cgcggagccg tcggatcgct
      481 gcgcgcgtgc tccgcgcccc gctgcgcatc cggccagcgc cagggccgcc gcaattatca
      541 gccccagggc gaattgcgag ctgcgaaata cgttcatcga tgaagcaagt tcaaactttg
      601 ccgcctgccg aatatgcgca gtgtggggca ggtttgttgc cgtaatcgag cgcagagcga
      661 tcgtaaggta ctccgataat atcgaacttc gatattgtag ggcttgaatc gaagtcagcg
      721 gctgcaccgg tcagagcgtc caaagcgaag tgccctgaag acgatttcct catcgatttc
      781 gcgacggcaa tcagcagcgc gacgcccgga agaagaatga ttgctgaccc gagcgggcca
      841 aaggcggctt ctgtcaccgg ccgccgtaac cgctggccgt aaagtgtccc cgcggcgacc
      901 cccccaattg ctgccgcgac aacccgcgac cgacgcaacc gtcgcggcga ccagcgccac
      961 gacaagtacc ctcagcaggg ccggcaagaa gccgagcatt gccacaactg gctactgcgg
     1021 ccaatccgct tgcggtttga aattgatcat cgccgaggcg ggccgctcgg gaatgtgcgg
     1081 gcggaggtac ggcttgtcag aagccttgtg gcaggaatag cacccttcga cgatgtactt
     1141 gtacgtcggt tcgaacttct ccgcgtcttg ccgcttgatc acctcaccca tctgcatgta
     1201 cggagagttt tcgatcgatt tcagaatgtc ttccagcttc acctctttgc cggcgttgtc
     1261 tttgcggacg ggaatgatcc gcaccgccca cttcatgtgc gccagcgatt ccttccagta
     1321 gaagtccgcc agcggccagt tgcgggcctt gccggcgtac caaagattgg agaagtggta
     1381 atcgacgtcg gacatggcgt gcgattgatc gggcacgacc cgtttgaggc gctcgacctc
     1441 ctgcgccaac gtggccagtg cgacaggcgg agcgttcgcc ggctgctggc cataggccgc
     1501 cgaaccggcc gtgcccaagt ccgaactcgc tgcacagagg aggacgccga gcagaccgcc
     1561 ggccagaaac gccgcgagga tgaaaaatgc cgccgagcga ttcatagaca tgtcctccga
     1621 aacgtggcag ggcaaaagat aacgtcccct acgatctggc cgccttcacc ccgatcggcg
     1681 gccttccagc tcgtcacttg caatgagctt ggccacacgc tgcttctcaa ccggcgctcc
     1741 gcgatagttc gccctcgccg gcgatctctg cgtgcttttg cgagtgcttt cttcgctcgg
     1801 tcatttcgtc ctgatcatcc acgatttcag aggccaattc gatcagcttg ccgcgcgctt
     1861 cgacaagcag ctttcgcccg cgggcagtcg tccgaaagtt ctttcgtcgt ttgccgccga
     1921 cgacttcgtc tttcgccttg acgaagccgg cttcttcgag gccatgcagc acggggtaca
     1981 gcgtcccggg gccgatgctg tagccgtggt gctgcaactc ctccatcaat tcgaccccgc
     2041 acacgggctg cttatcggcg tgatacagaa tgtgcaaacg cacaaagccg gtaaacaggt
     2101 gcttgaggac gttgttggcc ataatatcga aacgcgatat tgattgtcgt tcgcggcgta
     2161 gtaaaatcaa gcggtcgcct gctttgcccg taccccgcag cat
//
LOCUS       DASUGB010000928         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_938488, whole genome shotgun sequence.
ACCESSION   DASUGB010000928 DASUGB010000000
VERSION     DASUGB010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938488"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFV87_17620"
     CDS             <1..333
                     /locus_tag="VFV87_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105645.1"
                     /translation="ARVLRDGRPDLVLLSRRQEPSVAVMQSHEDEWVLLYQDELAQLW
                     GRATKYDDPASVYYLPPKKREVGDVVQRGWVHWPALPDYQPKPTPDTQSVPPDTQHLS
                     QAHVHGHR"
     gene            314..1204
                     /locus_tag="VFV87_17625"
     CDS             314..1204
                     /locus_tag="VFV87_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5105646.1"
                     /translation="MSTATADSTARPESLAAAPAPPDPIRVEDPITRVNYSLDQLQRL
                     ADLLAVEDGYEPAVREFEIPKHFKLSVVIPVYNEERTIQTVLQRVAALPVPKEIVIVD
                     DASTDGTRELLNQFECAADVHVIFKPQNEGKGAALRTGFRRVTGDVVVVQDADLEYDP
                     RDILAVIEPIVRGEADVVYGSRFLNVDGTRSVPTTFVHRLGNRMLTWASNLLTGLAIT
                     DMETCYKAFRREALRSFEIQQNRFGFEPEVTAKIARRKLRVEEVPIRYAARSYAEGKK
                     IGIKDLLNALWCIVRYGLWD"
     gene            complement(1624..2433)
                     /locus_tag="VFV87_17630"
     CDS             complement(1624..2433)
                     /locus_tag="VFV87_17630"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX5105647.1"
                     /translation="MNASWVSAVSLLALLSAVEVACADEKTPTKILFIGKQPDHPYGS
                     HMYLHTCGVLAKCAELTPGVEAVVSDGWPEDAEQFKDVKAIVVYTSPAADLLLGGPHR
                     DEVEKLLKSGVGLVTIHWASSVNKDNFERLGPAWLSYLGGTWVSNVGLSGGKSTLKQL
                     VPDHPICRGWKEYEFEDEYYLNPTIEKAKPLLAVTERGGKEVIVGWAHERDGGGRAFA
                     TTLGHPYRNFQDERFRRMIVNGILWAAHVEVPEEGASVDIGEDALALPPQK"
     gene            2598..3416
                     /locus_tag="VFV87_17635"
     CDS             2598..3416
                     /locus_tag="VFV87_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX5105648.1"
                     /translation="MHRISFFALAVTFTLAALAQAEGTCKVRGSVSSTEYEKLEGKLE
                     RSIVWVLADPDRTRLADTASTENKFEFDLKPGNYILQCTGNGSRGATFTPTYKKFTVT
                     EGAETLDLGTIDMPASETTKLFGQPAPELAGIVEWKNTDPRSLKELRGNVVILDFWSY
                     SCSICIHHMPDLAKLVEKHKGEKLVALTLHDNSAASLAEVDGILTVASKKKLGSLPVA
                     LDGQGADSVFRAYGIRATPAMILIDAEGNVVRRFHHAGDPQLDKEVTALLRAGR"
     gene            complement(3436..3705)
                     /locus_tag="VFV87_17640"
     CDS             complement(3436..3705)
                     /locus_tag="VFV87_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5105649.1"
                     /translation="MSLIYSVISWAVFGLVIGAIARFILPGKQSMSLLMTMLLGVVGS
                     FVGGGIAHLFGSGTGYVQPAGWIMSIVGAFILLAVYSYSKTKKAA"
     gene            complement(3834..4562)
                     /locus_tag="VFV87_17645"
     CDS             complement(3834..4562)
                     /locus_tag="VFV87_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105650.1"
                     /translation="MKRHWIWSGCAILALAVGCTQSSTLEDMQKAAENRMDEATEAVE
                     KATEDAVTKVEEAGAEAKTGLEEGTAAIEGAVKEGKAAIEGATEEGKAAVEGAAADAK
                     AGLEGSLGDLSALKVGDVEVGKECSAIAEKLKTTLASIKDVETAKEALPNLEEVNLGL
                     DKLTGLVEQIPEAVRPALATMIKTHSAKLTESIQRVVSIEGVGEIVKPVLDQIVAKLE
                     KVTGGAAPAEPAAGEAPAEAAPAP"
     gene            complement(4796..6361)
                     /locus_tag="VFV87_17650"
     CDS             complement(4796..6361)
                     /locus_tag="VFV87_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105651.1"
                     /translation="MASTSRRTFLQSVGSGMLVAGLGAGLVQDLGFSTAFANEGPETL
                     KFGKYDSLVDLMQSTPPDKLQALLIEKLNCGEADLKQLTVAAALANAETFGGEDYVGF
                     HTAMAMLPAWGMVNALPADRQPLPVLKVIYRNSDQIQKYGGADKKTLRERIAAEEATV
                     ENAGSVNTGLAIRDACRVADVEKGEQLFAAFENAPLEDAFNVLQPVMQDDINVHRFVF
                     AHRTYGLANMLGTDHAYTILRQCVRFCCSHEKNRIERKGQESPIRTVLPKLMDQYKLA
                     GKKLGKRDPGDAAVEELANAIYNGPPEKSAEAVAAALAEGMDVEVVGEAISLASNLLV
                     LRQGPDVWRAHGDSAGVHSSDATNAFRNMARHGDPQFAKSGLIVAAYHSAAHNAFKGE
                     MYPTTEHRSMIKMTDPEGLLAEAEDAVRHNDQGRAAAAIALYGEGGGSPEAVYERMLK
                     YTISEDGRLHGEKYFQTVQEEYKTTRPALRWRQIVGLARVTASAYGYDRQDKHGSRAP
                     GYEDACKLLKIEV"
BASE COUNT         1268 a         2286 c         1992 g         1285 t
ORIGIN      
        1 gcgcgcgtgc tgcgcgacgg ccggccggac ctggtgctcc tatcacgccg ccaggagccg
       61 agcgtggccg tgatgcaatc ccacgaggat gaatgggtgc tgctgtatca agacgagttg
      121 gcccagcttt ggggccgggc caccaaatac gacgatcccg cgagcgtgta ttacctgccg
      181 ccaaagaaac gcgaagtcgg cgacgtcgtg cagcgcggct gggttcactg gcccgccctg
      241 cccgactatc agccgaagcc gacgcccgac acgcaatccg tgcctcccga cactcaacat
      301 ctgagccaag ctcatgtcca cggccaccgc tgattccacc gctcgtcccg aatcgctcgc
      361 cgccgcgccc gcgccgcccg atccgattcg cgtggaagac ccgatcaccc gcgtcaatta
      421 ctcgctcgat cagctccagc gcctggccga tctgctcgcc gtcgaagacg gctacgagcc
      481 ggcggtccgc gaattcgaga ttccgaagca cttcaagctc tcggtcgtga ttccggtcta
      541 taacgaagag cggacgattc agacggtgct gcagcgcgtc gccgcactgc cggttcccaa
      601 ggagatcgtc atcgtcgatg acgccagcac ggacggcacg cgcgagctgc tgaatcaatt
      661 cgaatgtgcc gccgacgtgc atgttatctt caagccgcag aacgaaggca aaggcgccgc
      721 cctgcggacc ggctttcgcc gcgtgaccgg cgacgtggtc gtggtgcagg atgccgacct
      781 ggaatacgac ccgcgcgata tcctggcggt gatcgagccg atcgtgcggg gcgaggccga
      841 tgtggtttat ggctcgcgat tcctgaacgt agacggcaca cggagtgtgc ctacgacttt
      901 cgtgcatcgg ctgggcaatc ggatgctgac gtgggcttcc aacctgctga ccggcctcgc
      961 aatcaccgac atggaaacgt gctacaaagc gttccgccgc gaagccctgc ggagcttcga
     1021 gatccagcag aatcgcttcg gcttcgagcc ggaagtgacg gccaaaatcg cccgccgcaa
     1081 gctccgcgtc gaggaagtgc cgatccgcta cgccgcccgc agctatgccg aagggaagaa
     1141 gatcggcatc aaggacctgc tcaacgccct ctggtgcatt gtccgctacg gcttgtggga
     1201 ctgagtggcc cggccggatg acgaaggggt agaagccggg tcggtcatcg gcttattcgg
     1261 ctggggtatg ggagtggttg tggtgccggg gtcgaggcgc agctccctgg gctccgagcg
     1321 gacggcgttg gtcgcctgcg tgtctgcggg aatgaggttg gtgtggcaag atgggcaagt
     1381 ccaccaagga tgggagatct gcatgcgcac cttcaacttg cacgccggac agacgtagct
     1441 gtttggcgtc agtgctggat gcaaatttgg gacgttgctg tccatcgccg accacaattt
     1501 gggaaggcgc cctgctgggg cgcaccattc cccattgaat agtcggaatc ggcgcgcaaa
     1561 aacgggccga ggatcttctt ctttcttgtg aatttctcga attgaggcca attcgggcct
     1621 ggcttacttc tgtggcggaa gggcgagtgc atcttcgccg atatcgacgc tggctccttc
     1681 ctccggcacc tcgacgtgcg ccgcccacag gatcccgttg acgatcatcc gccggaatcg
     1741 ctcgtcctga aagttgcggt acggatggcc gagcgtcgtc gcaaacgccc gtccgccccc
     1801 gtcgcgctca tgcgcccagc cgacgatcac ctccttgccg ccccgctcgg tcaccgcgag
     1861 cagcggcttc gccttctcga tcgtcggatt caggtagtac tcgtcctcga attcgtactc
     1921 cttccagccg cggcagatgg gatggtctgg caccaactgc ttgagcgtcg atttgccgcc
     1981 gctgaggccg acgttgctga cccacgtgcc gccgagatag ctgagccacg ctggccccag
     2041 ccgctcgaag ttgtccttgt tcaccgacga ggcccaatgg atcgtcacca ggccgacgcc
     2101 gctcttcagc agcttctcga cctcgtcacg atgcggcccg ccgaggagca agtccgccgc
     2161 ggggctcgta tagaccacaa tcgccttcac gtccttgaac tgctccgcat cttccggcca
     2221 gccgtccgac accacggcct cgacgcccgg cgtcagctcc gcgcacttcg ccagcacgcc
     2281 gcacgtgtgc aaatacatgt gcgagccgta cggatggtcc ggctgcttgc cgatgaagag
     2341 gatcttcgtt ggcgtcttct cgtctgcaca ggcgacctca accgccgaca atagtgcgag
     2401 cagcgacaca gctgataccc acgatgcgtt cattcgcgga ccctcgcttc aggattagta
     2461 ggtgcaacag gtaacagaca cgtaacgtgg actctcgccg atcttgaagc atcgccggct
     2521 tccgacgaaa accaaacaca gcggctatcg tctcattgtc agtccctgag cgtcaaagaa
     2581 aaaccaggag ttcgattatg caccggattt cgttcttcgc tctagctgtc actttcaccc
     2641 tcgcggcact ggcgcaggcc gaagggacct gcaaggttcg cggcagcgta agcagcaccg
     2701 aatacgaaaa actggagggc aagctggagc gttcgattgt gtgggtgctc gccgatccgg
     2761 accggacgcg tctggccgat acggcatcga ccgagaacaa attcgagttc gatctcaagc
     2821 cgggcaatta catcctgcag tgcacaggca acggcagccg cggggcaacg ttcacgccga
     2881 cctacaaaaa attcacggtc accgaaggcg ccgagacgct ggacctcggc acgatcgaca
     2941 tgccggccag cgaaaccacg aagctgttcg gccagccggc gcccgagctg gcgggaatcg
     3001 tcgaatggaa aaacacggac ccgcgctcgc tgaaggagct ccgcggcaac gtcgtcattc
     3061 tcgatttctg gtcgtactcc tgctcgatct gcatccatca catgccggac ctggcgaagc
     3121 tcgtcgagaa gcacaagggc gaaaagctcg tcgccctcac gctgcacgac aactccgccg
     3181 ccagcttggc cgaagtggac ggcattctaa ccgtcgcctc gaagaagaag ctcggcagcc
     3241 tccccgtcgc cctcgacggc caaggcgccg acagcgtctt ccgcgcctac ggcatccgcg
     3301 ctacgcccgc gatgatcctg atcgatgccg aggggaacgt cgtccgccgg tttcatcatg
     3361 ccggcgatcc gcagctcgac aaggaagtca ccgcgctgct ccgtgcaggt cgctagcgtt
     3421 cagccgcgcg ctcgtttacg ccgccttctt cgtcttcgaa tacgaataga cggccaggag
     3481 gataaaggcg ccgacgatcg acataatcca gcccgccggt tgaacgtagc cggtcccgga
     3541 gccgaacagg tgcgcaatcc cgccgccgac aaacgagccg acgacgccca ggagcatcgt
     3601 catcagcagg ctcatcgact gtttgccggg caggatgaac cgggcgatcg cgccgatcac
     3661 cagcccaaac acggcccaac tgatcacaga atagatgagc gacatgtgca tttctcccga
     3721 acgggaaatg gaagcgagat aggtcgaacc cggtgacagc ggcgctcacc cgccgcctgg
     3781 gccgcgggag ccggctgctc ccgctacgat tcgctcggcg attcttcaaa cgcttaagga
     3841 gccggcgcgg cctctgcggg cgcctcgccc gccgccggtt cggccggcgc ggcgccgccc
     3901 gtgaccttct ccagcttggc gacaatctgg tcgagtactg gcttgacgat ctcgccgaca
     3961 ccttcaatcg acacaaccct ctggatcgac tcggtaagct tcgccgagtg ggtcttgatc
     4021 atcgtcgcca gcgccggtcg gactgcctcg ggaatctgct cgaccaggcc ggtgagctta
     4081 tcgagtccca aattcacttc ttccagattc gggagcgcct ccttggccgt ttccacgtcc
     4141 ttgatgctgg ccagcgtcgt cttcagtttc tccgcaatcg cgctgcactc tttgcccact
     4201 tcgacatcgc ccaccttcag cgccgacaaa tcgccgagag acccttcgag cccggctttc
     4261 gcgtccgcag ccgcgccttc cacggccgcc ttgccttcct ccgtggcgcc ttcgatggcg
     4321 gccttgcctt cctttaccgc accctcgatg gcggccgtcc cttcctccag acccgtttta
     4381 gcttccgcgc cggcttcctc caccttcgtt acggcatctt cagtggcctt ttcgaccgcc
     4441 tcggtcgctt cgtccatcct gttttccgcc gccttctgca tatcctccag cgtggacgat
     4501 tgcgtgcagc cgactgcgag cgccagaatt gcgcacccgc tccaaatcca atgacgtttc
     4561 atggccagaa ctccgagcaa agagaagcgc accccacgac cagcacggcc gcgcgaggtt
     4621 gacagccgct cattatacga cttgggcgcg tctcgcaacg gtaggcttct cgctccgcga
     4681 gaagcgatgc aacctccatc cggatggact ttgcgcaacg aacagccggc gcaatcgcgc
     4741 cgggcctggt tcgtcaaaca gatcgatcgc ctctcgcgga gcgagaggcc tacgatcaca
     4801 cctcgatctt cagcagcttg caggcatcct catagcccgg cgcccggctg ccatgcttgt
     4861 cttgccggtc gtagccatag gcactggccg tgacgcgggc caggccgaca atctgccgcc
     4921 agcggagcgc tggccgtgtg gttttgtact cctcctgcac ggtctggaag tatttttcgc
     4981 cgtgcaagcg gccatcctcg ctgatcgtgt acttgagcat tcgctcgtac acggcctcgg
     5041 gcgagccgcc tccttcgcca tacaaggcaa tcgccgcggc ggctcgcccc tggtcgttgt
     5101 ggcgaacggc gtcctccgct tcggcgagca agccttccgg atcggtcatc ttgatcatcg
     5161 accgatgctc ggtggtggga tacatctccc ccttgaacgc gttgtgagca gccgagtgat
     5221 aggcagccac aatcaggccg ctcttggcga actgcggatc gccgtggcgg gccatgttgc
     5281 ggaaggcgtt cgtcgcgtcc gacgaatgca cgccggccga atcgccgtgc gcccgccaca
     5341 cgtccggccc ttgccgcagg acgagcaggt tcgacgccag cgaaatcgcc tcgccgacca
     5401 cctccacgtc cattccttcg gccagggcgg cggcgacggc ttcggcggac ttctcgggcg
     5461 ggccgttgta gatggcattc gccagttctt cgaccgctgc gtcgcccgga tcgcgcttgc
     5521 cgagcttctt gccggccagc ttgtactggt ccatcaactt gggcaggacc gtacgaatcg
     5581 gcgactcttg gcctttccgc tcgatcctgt tcttttcgtg cgagcagcag aaccgcacgc
     5641 actgccgcag gatggtgtag gcgtggtcgg tgccgagcat gttggccagg ccataggtgc
     5701 gatgggcgaa tacaaaccgg tggacgttga tgtcgtcctg catgaccggc tgcagcacgt
     5761 tgaacgcgtc ttccagcggg gcgttctcga aagccgcgaa gagctgctcg cccttttcga
     5821 cgtcggccac gcggcaggcg tcgcgaatcg ccaggccggt attaacgctg ccggcgttct
     5881 cgacggtcgc ctcttccgcc gcaatccgct cgcggagcgt cttcttatcc gcgccgccgt
     5941 acttctgaat ctggtccgaa ttgcgataga tgaccttcag caccggcagc ggctggcgat
     6001 cagccggcag cgcattgacc atcccccagg ccggtagcat ggccatcgcc gtgtggaagc
     6061 cgacgtagtc ttcgccgccg aacgtctcgg cattggccag ggccgccgcg accgtgagct
     6121 gcttcaggtc cgcctcgccg cagttgagct tctcgatcag cagggcctgc agcttatccg
     6181 gcggcgtgct ctgcatcaga tcgaccagcg aatcgtactt gccgaacttg agcgtctccg
     6241 gcccttcatt ggcaaaggcg gtcgagaacc cgaggtcctg caccaaaccg gcgcccaacc
     6301 cggccaccag catgccgctg ccgaccgact gcaagaacgt gcggcgagaa gtagaagcca
     6361 tgattcgtct ccgagcgaag aaggggagag ggataacggt tagcctaacg gttcgacagc
     6421 caaaagtcac acaaacccga cgcgcaagcg agggcccaca caaacccgca gcctcagcga
     6481 gggctcacag aaacccgaag cgtcagcgag ggaatcaatg cacgcctcga ttgcctcacc
     6541 acttacaacg cattcgcgtt acagccccgc tcgcgtctcc ccactttcag tcgctccttc
     6601 tacagttctc catgttgact gaacagtccc gctttcaagt cacgaggtgc ttcttaggcc
     6661 taaactttgg cgtgttcttt cgccacacga acccaatcgg aatagccgta aaccactaat
     6721 caacaaaggc ttccacgcca aactcgatac gccattctac acatgcggtt tagcgcggtt
     6781 tagcgccaga ctccgacgca atcgaaatcg ttgaacattc cctaatcagg g
//
LOCUS       DASUGB010000929         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1076594, whole genome shotgun sequence.
ACCESSION   DASUGB010000929 DASUGB010000000
VERSION     DASUGB010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076594"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFV87_17655"
     CDS             complement(<1..216)
                     /locus_tag="VFV87_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105652.1"
                     /translation="MHDVGTGDDDGSEKLGPKYDTPTLLGVYRTAPYLHHGQAATLRE
                     VLANNPGDRHGTTSHLSATQIDDLVEFL"
     gene            complement(194..871)
                     /locus_tag="VFV87_17660"
     CDS             complement(194..871)
                     /locus_tag="VFV87_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5105653.1"
                     /translation="MLDISRDGTLLACANRDSGTVTIVELVSNTKVAETAVGKHPEGV
                     SFLGESHRLAVAVYDDDRVVLLNAETGAITHRIDVFDEPYGVVSNRDGTRAFVSLDYP
                     GQVVEIDTAAEKIVRTFEAGEFARGIALSPDQSRLFVTQYYSGSVLAIDLVSGKIVDQ
                     WLGISNDNLARQIAIHPLRDKAERCCSARQVALLLGRNQVRHVPQWAAVYRLGSQGDA
                     DARRGHG"
     gene            1372..>2469
                     /locus_tag="VFV87_17665"
     CDS             1372..>2469
                     /locus_tag="VFV87_17665"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105654.1"
                     /translation="MVPIICSRRGFLQAGALGVGGLTLAELSRARACGAVPSRAAGTS
                     VILFWLSGGPGHMETWDPKPSAPAEYRGPLLAIDTSVPGIQFSELMPEQARLMDRLAV
                     LRTVSHGSGDHTKGNHWMLTGFEGPAFNAPDNRQQRRPALGSAAARVRGANQPGMPPY
                     VAVPHLRGGTDNLFHYAAYLGGGWNPFVVNSDPNERDFAVRNLTLQSDLSLQRIADRR
                     TLREELDRWQQSAERDMRDLDEHQQSALDLLSSRRVRDAFDISQEPDTLRDQYGRHTF
                     GQSALLARRLVESGVTFVTVNCVPWDHHGSPGQYKTEEGAKLLIPPLDRAIAGLIRDL
                     IDRGLYDKTLVVAMGEFGRTPQLGTQGSTDGR"
BASE COUNT          448 a          764 c          797 g          460 t
ORIGIN      
        1 caggaactcg accaggtcat caatctgagt ggcggaaagg tggctggttg tgccgtggcg
       61 gtcgccgggg ttattggcga gcacctctct cagcgtggct gcctggccgt gatgcagata
      121 gggggcggtt cgatagacgc cgagcaacgt gggcgtatcg tacttcggac cgagtttctc
      181 gctgccatca tcgtcacccg tgcccacgtc gtgcatcagc gtcgccttga cttccgagtc
      241 ggtaaacagc ggcccactgt ggcacgtggc gcacttggtt tctgccgaga agaagagcga
      301 cttgccgcgc tgagcagcag cgctcagcct tgtcgcggag tggatggatg gcgatctggc
      361 gggccagatt gtcgttgctg atgccgagcc actgatcgac aatctttccg ctgaccaggt
      421 cgatcgccag caccgagccg ctgtaatact gcgtcacgaa gagccgcgac tggtcgggcg
      481 acaaggcgat gccccgtgcg aattcgccgg cctcgaacgt ccgcacgatc ttttctgccg
      541 ccgtatcgat ctcgacaacc tgtcccggat aatcgagtga gacgaaggcc cgcgtaccat
      601 cgcgattgct gaccacgccg tacggctcgt caaagacgtc gatccggtgc gtgattgcgc
      661 ccgtctcggc attgaggagg acgacgcgat cgtcgtcata aacggcgact gccagccggt
      721 ggctttcgcc gagaaaactg actccctcgg gatgtttgcc aacggcggtc tccgcgacct
      781 tggtgttcga aaccaactca acgattgtga cagtgccgct gtcgcggttg gcgcaggcca
      841 ggagcgtgcc gtcgcgagag atgtcgagca agctgttgct gtcgcctgtc gaaacaggcg
      901 cggacaagcc ttgagtgctg aacgatctgc gcaaaacatg tgcaacatat tacgacgctc
      961 acttgccgga atcttcgaac gaaaccctgg ggcacactag tccaagcttc gccatggtca
     1021 tcgtccgcac tgaagacaaa attcgcgtcg ccaagcaggc ccgagtagaa gggatgccca
     1081 cttcctctcg ttatcgctgt gcaacagagg cacgatggtg cagcgctgtg ttgggcgagg
     1141 gttccttcgt ggcctgcttg ttgcaattgc tcgtattcga ctgactctgg agtcggcacg
     1201 ccgctttcag cggaagtgca tgccgcggtg ctcgggacgt gcgacgcgaa tgttggcacg
     1261 cgggtcgtcg cggtgcgaag gtgcattgtt tcttgccacg gttaatccat taggatacgt
     1321 tgatggtacc agccggctgg tcagaattcc tgtatgcgat cggattgctc catggtgccg
     1381 atcatctgtt ctcgccgagg atttctccaa gccggcgcgc tgggggtggg cggcctgacg
     1441 ctcgccgaac tatcgcgggc gcgcgcttgc ggagccgtgc catcgcgagc cgccggcaca
     1501 agcgtcatct tgttctggct cagcggcggc cccggtcaca tggaaacctg ggatcccaag
     1561 cccagcgccc ccgccgagta tcgcgggccg ttgctggcaa tcgacacgtc cgttccgggc
     1621 atccagttca gcgaactgat gcccgagcag gcgcggctga tggaccggct ggcggtgcta
     1681 cggacggtga gccacggctc gggcgaccac accaagggga accattggat gctcacaggg
     1741 tttgaagggc cagccttcaa cgcccccgac aaccgccagc agcgccggcc ggcgctgggt
     1801 tccgcagccg cgcgggtgcg cggggcgaat cagcccggaa tgccgcccta cgtggcagtg
     1861 ccgcacctgc gcggcggaac cgacaatctg tttcattacg ccgcgtacct gggcggcggc
     1921 tggaatccgt ttgtggtcaa ttcggacccc aacgaacgag atttcgccgt ccgcaacttg
     1981 acattgcaga gcgacctttc cctgcagcgc atcgccgacc ggcgcacgct ccgcgaggag
     2041 ctcgatcgct ggcagcagtc ggcggagcgg gatatgcgcg atctcgacga gcaccagcag
     2101 tcagctctgg acctgctctc cagccgccgg gtacgggacg ccttcgacat ctcacaagag
     2161 ccggacacgc tgcgcgacca gtacggccgg catacttttg gccaaagcgc cctgttggcc
     2221 cgccggctgg tggaaagcgg cgtgacgttc gtcacggtga attgcgtgcc gtgggaccat
     2281 cacggatcgc cgggccaata caagacggaa gaaggggcga aactgctcat tccgccgctc
     2341 gaccgcgcga tcgccggact gattcgcgac ctgatcgacc gtggactgta cgacaagacg
     2401 ctggtggtcg cgatgggcga gttcggccgc acgccgcaac tcggcacgca gggcagcacc
     2461 gacggccgc
//
LOCUS       DASUGB010000930         7272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1215583, whole genome shotgun sequence.
ACCESSION   DASUGB010000930 DASUGB010000000
VERSION     DASUGB010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215583"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFV87_17670"
     CDS             complement(<1..694)
                     /locus_tag="VFV87_17670"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX5105655.1"
                     /translation="MTSTQRIGVLTAGGDCPGLNAVIRGVVKSANTHGYEVVGFLRGY
                     EGLVDPVSFIPLTHKNTTGILSQGGTILGSTNRGRFAATVGVNDRLELDPELVAGVKT
                     TVEQLNLSGLICVGGDGSLAVAQQFHENGIPVVGVPKTIDNDLSSTAFTFGFFSAVFC
                     ATDALDRLHTTAASHERIMVLEVMGRHAGWIALYAGIAGGGDVILIPEIPWSFDNVCQ
                     AIISREQRGKRFT"
     gene            1076..2119
                     /locus_tag="VFV87_17675"
     CDS             1076..2119
                     /locus_tag="VFV87_17675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5105656.1"
                     /translation="MPQPTPAEELIQTDVFTSGSEGYHTYRIPALVVTKKGTLLAMCE
                     GRKTGRGDHGDLDLIQKRSTDGGQTWGKLERIHEEGGDAKITIGNPCPVVDQETGVIW
                     LPFTRDNDKVFVTSSSDDGQTWTAPREITADVKSPDWNWYATGPGNGIQLVHGKHKGR
                     LVIPCDHRVQGASNEWNHAGRSHVIFSDDHGQTWKLGGSTDFAMNECAVVELPGATLM
                     LNSRSYRGKSRRGVSLSKDGGQTWEATTDDPALFEPVCQASLIRYSPDVLLFSNPADA
                     KGRHHLTVRASNDQGKTWPRSRMICEGSSAYSSLAALPGGGIGLLYERDNYQHLTFVR
                     FPLDSIQTNAAAK"
     gene            2248..4023
                     /locus_tag="VFV87_17680"
     CDS             2248..4023
                     /locus_tag="VFV87_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5105657.1"
                     /translation="MLPRIFALFFLSSIFLSASAADERDRSPVDLVLAPDESWLATVN
                     QTSNTVSLVRTSDGQVLDEVAVGEHPIAISLAPDSKTLLVSGHYSGDVSLLEVQGEKL
                     AKIGSIDVGFHPHDIAVTADGKSAFVARTASAEIAIVDLIDRKVIGAIPVGRWPRHLA
                     LSPDGTRLAVGTSGDRGVTVVDIPNRTALYQEQFVGLNIGHMVITKDGQHVYFPWMVY
                     RNNPIAAGNIRLGWVLASRVARMRLDIAARREAMSLDPQGKAIADVTGIGLTSDDSHL
                     VVSASGTHELLVYRTEGLPLKDYGGTDHIDPELLKDQDRFFRIETGGRPMGLKIAQDD
                     RTVYVADYLGNCVQVVDLNDRALSRTISLGGPAAPSLARQGEAIFYDARRSLDQWYSC
                     HTCHYEGGTTSVVTDTTNDGSAYTFKTVLQLYNLHETAPWTWHGWQTDLRAGIRKSLT
                     ETMLGPQPTEEDVDAMLAYFKTLPEPPNPFRNRDGSLSAAAERGKSIFESDKAACASC
                     HNGPHFTDGQIHDVGLGARTDRYKGYNTPSLRGLYRRVKLLHNGQVDSLEALLSGPHA
                     PENVAGTDKLSEQEVRDLVEYLKTL"
     gene            complement(4096..4614)
                     /locus_tag="VFV87_17685"
     CDS             complement(4096..4614)
                     /locus_tag="VFV87_17685"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEX5105658.1"
                     /translation="MIPLLVHNWQIVLFGVIAAALFVVAMLALRDKLPPYERRGGLLT
                     PAGINFFRTLQLAAREDWVIFSMVRLADIIKVRPKTRKSLLWNNRIQGKHIDFVVCDY
                     ETLEVKLAIELDDDLVPRSQRRARDNFLTVALTAAGVPLLRVKVREKYETAAVRKDIE
                     DALGIVRKKKRA"
     gene            5028..6683
                     /locus_tag="VFV87_17690"
     CDS             5028..6683
                     /locus_tag="VFV87_17690"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5105659.1"
                     /translation="MLDTAEIDSFVARPRPPVDRSTELSRCAAALHQTFGVTFSVWDG
                     ETGELLHASTGQPASNDPIRGQLARAIQGREPQFLADEDCVLLLAVPLVLDEERTIVA
                     TAAFATRRVEGGESPNGATRLLGLSPAAAVIWIRQQTIWPPDALLRLAAAVQAQLAAE
                     ERARRAEREVEEISDNLASTYEEICLLHGVTQNLRISSDEEQLSNLVLRWLLDCLPVA
                     GVAIQLLPVAKEGNVTYKARTRSSLVTAGQCPLDNDAFTQLVESLGLEAGCGPLVANG
                     AVTGGEDWAFPTVRQLIVVPMAEGDRVFGWLAAINHAEDAEFGTIEASLLNSIGAMLG
                     IHASNRDLYRQQSEFLASVVRALTSAIDAKDPYTCGHSDRVARIAVRLAKELGCEQEA
                     LQTLYMAGLLHDIGKIGIDDAVLRKPGQLTFEEFEHIKQHPELGYRILADIKQLADVL
                     PAVLHHHEQWDGRGYPCKLAAEQIPLIARIVAVADAFDAMTSDRPYRPGMPVHKVDEI
                     FKSGGGKQWDPEVIAAFFAARQDILDISENERANLTLDVQQWT"
     gene            6707..7192
                     /locus_tag="VFV87_17695"
     CDS             6707..7192
                     /locus_tag="VFV87_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105660.1"
                     /translation="MSRSESPRDDVFAEAKALVERAELTIPGHADSIVVGFRRDGSAS
                     FFFGGDPVYHFNTAGELRRAYIGDLLYKAEHGRLIALRRERSAWVTALVRHELTPAET
                     VSILSAMRQRLLQLLDALNGNDAALLRQAPSDVDLAGRLRDWLSQMPAVIEVARVAGL
                     R"
BASE COUNT         1335 a         2376 c         2259 g         1302 t
ORIGIN      
        1 gcgtgaaccg cttgccccgc tgttcgcggc tgatgatcgc ctggcagacg ttatcgaacg
       61 accagggaat ttcgggaatc aagatcacat cgccgccgcc ggcgattccc gcatacagcg
      121 cgatccagcc cgcgtgccgg cccatgacct ccagcaccat gatccgctcg tggctggcgg
      181 cggtagtgtg caggcggtca agggcgtcgg tcgcacaaaa gacggcactg aagaagccga
      241 atgtgaaggc cgtgctcgag aggtcgttat cgatcgtctt cgggacgccg acgaccggga
      301 tgccattttc gtgaaactgc tgggccaccg ccagcgagcc gtcgccgccg acgcagatca
      361 gcccggacag attaagctgc tcgacagtcg tcttcacgcc cgccaccagc tccggatcca
      421 gctccagccg gtcgttcacg ccgacggtcg cggcaaagcg gcccctgttc gttgagccga
      481 ggatcgtccc cccctggctc agaatgcccg tcgtgttctt gtgcgtcagc gggatgaagc
      541 tgaccggatc gaccagccct tcgtatcccc gcaaaaagcc caccacctca tagccgtgcg
      601 tgttggcgct tttgacgacg ccgcgaatga cggcgttgag cccggggcag tcgccgccgg
      661 cggtgaggac gccgatgcgc tgagtactgg tcatgtgctc tctccggtag gccggggcgg
      721 ggcgtggcgc cgcacggcgg tcagggcgtt ggtcggtcac ttgcgcacct atggccaggg
      781 tcgatttgat ctggcaaatc taggttacgc ctcccggccg agccaattgg cgcttgcgtc
      841 gtaacccgcc cagccggaat atggagaggc tgactgtcac tctatccgtt tcggcagaga
      901 acgcaaagac gccaagaagc gccaaggcgc ggggaggaac gaagtgaaag gcattccatt
      961 gggccgtggc caatcaagca gacccctttc cctgccttgc ctctctgcgc ctttgccttt
     1021 cctttgcgcc tttgcgtcga atcgaacgat cctagcgctg cttctcacgc tcgccgtgcc
     1081 gcagcccacc ccggcggagg agctgatcca gaccgacgtc tttacctccg gcagcgaggg
     1141 ttatcacacc tatcgcatcc cggcgctggt ggtgacgaag aaagggacgc tcttggccat
     1201 gtgcgaagga cgcaagaccg gccgcggcga ccatggcgat ctcgacctga tccagaagcg
     1261 cagcaccgac ggcggccaga cctggggcaa gctggaacgg atccacgagg aagggggcga
     1321 cgcgaaaatc acgatcggca atccgtgccc ggttgtcgat caagaaaccg gcgtcatctg
     1381 gctccctttc acgcgcgaca acgacaaggt tttcgtcacc tcgtcgagcg acgacggcca
     1441 gacgtggacc gcgccgcgcg agattacagc cgacgtaaaa tcgcccgact ggaactggta
     1501 cgccactggg cccggaaatg ggatccaatt ggttcacggc aagcacaaag gccggctggt
     1561 gatcccctgc gatcatcgtg tgcaaggcgc cagcaacgaa tggaaccatg ccggccgatc
     1621 gcacgtgatt ttctccgacg atcacggcca gacgtggaag ctcggcggct cgaccgactt
     1681 cgccatgaac gaatgcgcgg tcgttgagct tcccggcgcc accctcatgc tcaacagccg
     1741 cagctatcgc ggcaaatcgc ggcgcggagt ttctttgagc aaggacggcg gccaaacctg
     1801 ggaggcgaca acggacgatc cggcgctctt tgagcccgtc tgccaggcca gcctgatccg
     1861 ctattcgcct gacgttttgc tgttcagcaa tccggccgac gccaaaggcc gccaccattt
     1921 gaccgtccgc gccagcaacg accagggaaa gacctggccc cgctcgcgaa tgatctgcga
     1981 aggctcgtct gcttattcct cgctcgcggc cctccccggt ggaggaatcg gcctgctgta
     2041 cgagcgcgac aactaccagc acctcacgtt cgtccgcttc ccgctggatt cgattcagac
     2101 gaatgctgct gcgaaatagc ccgcatccct tacaatagcc gggagcgctt tgtcgccttt
     2161 cgctccgcga aagggcgttc tttcgcggag cgaaagacga cattcagcga agagccattc
     2221 cccccacctg ccacgcaagc ccacctcatg ctcccacgta tcttcgccct ctttttcctg
     2281 tcttcaatct tcctgtccgc ctccgcggct gacgagcggg atcgctcgcc ggtcgatctg
     2341 gtgctggcgc ccgatgaatc gtggctggcg acggtcaacc agaccagcaa cacggtttct
     2401 ctcgtccgca cgagcgacgg gcaggtgctg gatgaagtcg cggtcggcga gcaccccatc
     2461 gccatctcgc tcgcgccgga cagcaagacg ttgctcgtca gcgggcacta ttcgggcgac
     2521 gtgtcgctgc tggaggttca gggggagaag ctcgccaaga tcggctcgat cgacgtcggc
     2581 ttccacccgc acgacattgc cgtgaccgcc gacggcaaat cggcatttgt ggcccgcacc
     2641 gctagtgccg aaatagcgat cgtcgatctg atcgatcgca aggtaatcgg cgccatcccc
     2701 gtcggccgtt ggccccggca tctggccctg tcgccggacg gcacgcggct ggccgtcgga
     2761 accagcggcg accgcggcgt gacagtcgtc gatatcccca accgcaccgc gctttatcag
     2821 gagcagttcg tcgggctgaa tatcggtcac atggtgatca ccaaggacgg gcagcacgtc
     2881 tatttcccct ggatggttta tcgcaacaac ccgatcgccg ccggcaatat ccgcctgggc
     2941 tgggtcctcg ccagccgcgt ggcccgcatg cggctggata tcgcagcccg gcgggaggcg
     3001 atgtcgctcg atccccaggg gaaggcgatc gccgacgtca ccggcatcgg cctgaccagc
     3061 gacgattcgc atctggtcgt ctcggcttcc ggcacgcatg aattgctggt gtatcgaacc
     3121 gaagggctgc cgctaaagga ctacggcggc accgaccaca tcgaccccga gctgctcaag
     3181 gaccaggacc gctttttccg gatcgagacc ggcggccggc cgatgggcct gaaaatcgcc
     3241 caggatgatc ggaccgtcta tgtggccgat tatctgggca actgtgtcca agtcgtcgat
     3301 ctgaacgacc gggccctcag ccgcacaatt tccctcggcg gcccggcggc gccctcgctc
     3361 gcccgtcaag gcgaagcgat cttctacgat gcacgccgca gtctcgacca gtggtatagt
     3421 tgccacacct gccactacga agggggcacg accagtgtcg tcaccgacac gacaaacgac
     3481 ggctcggcct acacgtttaa aacggtgctg cagctctaca atctgcacga aacggccccc
     3541 tggacctggc acggctggca gacagacctg cgtgccggca ttcgaaaaag cctcaccgag
     3601 actatgctcg gcccacagcc caccgaggaa gacgtggacg caatgttggc ctatttcaaa
     3661 acgctgccag agccgccgaa tccgtttcgc aaccgggatg gctcgctcag cgcagcagcc
     3721 gagcgcggca agtcgatctt cgagagcgac aaggccgcct gcgcgagctg tcacaatggc
     3781 ccgcacttca ccgacgggca gattcacgac gtggggctcg gcgcgcggac ggaccggtac
     3841 aagggctaca acacgccgag cctccgcggc ctttatcgcc gcgtcaagct gttgcacaac
     3901 ggccaggtcg attcgctcga ggcgctgctc tccggcccgc atgctccgga aaacgttgcc
     3961 ggcacggaca agctcagcga gcaggaagtg cgggatttgg tcgagtatct gaagacgctg
     4021 tgatccgcgt aggacgagtt tccaacccgt ccctgccgcc tatgtggacg ggttggaaac
     4081 ccgtcctacg tggctttacg cccgtttctt cttccgcacg atgcccaggg cgtcctcgat
     4141 gtccttgcgc acggcagccg tttcgtattt ttcgcggact ttcacgcgga gcagggggac
     4201 gccggcggcg gtgagggcga cggtcaagaa attgtcgcga gcccgccgct gcgagcgggg
     4261 caccaaatcg tcgtctaatt cgatcgccag ctttacctcc agcgtctcat agtcgcagac
     4321 cacgaagtcg atgtgcttcc cctggatgcg gttgttccac agcagcgatt tgcgcgtctt
     4381 gggacggact ttgatgatgt cggccaggcg aaccatcgag aagatgaccc agtcctcgcg
     4441 agccgcgagc tgcaacgtgc ggaagaaatt aatccctgcc ggcgtcagca gcccgccccg
     4501 ccgctcatag ggtggcagct tgtcgcgcag ggcgagcatg gccacgacga acagcgccgc
     4561 ggcgataacg ccaaagagga cgatctgcca gttgtgaacg agaagcggaa tcatgggccg
     4621 tcggacagtt cttaagctgc cgtccgggac gggttggaaa cccgtcctac gtttcatcgc
     4681 gggagtagcg ctttatctgt catcggagat gcgctgccag gggctgcacc ggatgctggg
     4741 gcggtcaatc cgggcggtcg gcatcggacg cctgtgacgg tgctgacgat ggtaacggac
     4801 ttgtcgaatc taccactggc gagagtcgcg ccggatcgcg gtccgttccc gcccgccgcg
     4861 ctttgcgcag gccgccaact cgcagcctcc ccgcgaacta ggctttctca gaattcccac
     4921 cggtccgcgc cgacggccgg aatgtcgtcg ttcgctccgc gaacgaacgc cctttcgcgg
     4981 agcgaaaggc gacattttga tttggccaaa aaactccgtc gtctccaatg ctggacaccg
     5041 cagaaatcga ttccttcgtc gccaggccgc gtccgccggt ggaccggagc acggagctgt
     5101 cgcgctgcgc cgccgccttg caccaaacgt ttggcgtcac tttcagcgtc tgggatggcg
     5161 aaacagggga gctgctgcac gcttcgaccg ggcagccggc gagcaacgac ccgatccgcg
     5221 ggcagttggc ccgcgcgatt caggggcgcg agccgcagtt cctggccgac gaagactgcg
     5281 tcctcttgct cgcggtgccg ctggtcctgg atgaggagcg gacgatcgtc gccacggctg
     5341 cctttgcgac ccgccgcgtg gaaggaggcg agagtcccaa cggcgcgacc aggttgcttg
     5401 gcctgtcgcc cgccgcagcc gtgatctgga tccgccagca gacgatttgg cctcccgatg
     5461 ctttgctgcg gctggcggcc gcagtgcaag cgcagcttgc cgccgaagag cgtgcccgcc
     5521 gcgcggagcg cgaggtcgag gagatttccg acaatctggc ctcgacctac gaagaaatct
     5581 gcctgctgca cggcgtcacc cagaatctgc ggatcagcag cgacgaagag cagctctcca
     5641 atctcgtcct gcgctggctg ctcgattgcc tgccggtcgc tggcgtggca attcagcttc
     5701 tgcccgtcgc caaggaaggg aatgtcacgt acaaagcccg cacccgctcg tcgctggtca
     5761 cagccgggca gtgcccactc gataacgacg cctttacgca gctcgtcgag agcctgggtc
     5821 tggaagccgg ctgcgggccg ctggttgcca acggtgcggt cacaggcggc gaggactggg
     5881 ccttccccac ggtgcggcag ctcattgtcg tgccgatggc cgaaggggac cgtgtgttcg
     5941 gctggctggc ggcgatcaac cacgctgaag acgccgaatt cggcacgatc gaggccagcc
     6001 tgctgaattc gattggggcg atgctcggca tccacgcctc caaccgcgat ctgtaccgcc
     6061 agcaatcgga gtttctggcc tccgtggtgc gggctctcac cagcgcgatc gacgccaaag
     6121 acccctatac ctgcggtcat agcgaccgcg tcgcccgcat tgccgtgcgg ctggccaagg
     6181 agctgggctg cgagcaggaa gcgctgcaaa cactgtacat ggcgggcctc ttgcacgata
     6241 tcggcaagat cggcatcgac gacgcggtgc tccgcaagcc cggccagctc acgttcgagg
     6301 aattcgagca catcaagcag cacccagagc tgggctaccg cattttggcg gatatcaagc
     6361 agcttgcgga cgtccttccc gcggtgctgc accaccacga gcagtgggac ggccgcggct
     6421 atccgtgcaa gctggccgcg gagcagattc cgctcatcgc ccgcatcgtg gcggtggccg
     6481 acgccttcga cgccatgacc agcgaccgtc cctaccgccc tggcatgccg gtccacaagg
     6541 tcgatgagat cttcaaatcc ggcggcggca agcaatggga tcccgaggtc atcgccgcct
     6601 tcttcgccgc tcgccaggac atcctcgaca tctccgaaaa cgagcgggcc aatctgacgc
     6661 tcgacgttca gcagtggacg tagcgcaggc gcgttaaaat taggggatgt cccgttccga
     6721 gtctccacgc gacgacgtct ttgctgaagc caaggcgctg gtcgagcgag cggagttgac
     6781 aattcccggc catgctgact cgatcgtagt gggttttcgt cgagatggtt cggcgagctt
     6841 cttctttggc ggagatcccg tctatcattt caacaccgcc ggcgaattgc ggcgggccta
     6901 catcggcgat ttgctctaca aagccgaaca tggccgcctg atcgctttgc gccgcgagcg
     6961 gagcgcgtgg gtaacagccc tggtccggca cgagctgacg cccgcggaaa cggtgtcgat
     7021 cctgtccgct atgcggcagc gcctgcttca attgctcgac gcgctgaatg gcaacgacgc
     7081 ggctttgctg cggcaagctc cttccgatgt cgatctagca ggtcgacttc gcgactggct
     7141 gagtcagatg cccgcagtga ttgaggtggc gcgtgtggcc ggcctgcggt gagccgaaag
     7201 tgggcggcga gcgtgcccgc gctgacgtcg cgcggtacac tacagacagc ttgtctcgac
     7261 tattcgtttc ca
//
LOCUS       DASUGB010000931         8789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1354306, whole genome shotgun sequence.
ACCESSION   DASUGB010000931 DASUGB010000000
VERSION     DASUGB010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354306"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2488
                     /locus_tag="VFV87_17700"
     CDS             <1..2488
                     /locus_tag="VFV87_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105661.1"
                     /translation="NKLGKPMEASAIFNELLANPDSPEAFRTLKVKVMALALEAWNPQ
                     GLYLEIIDKGGKLVDSARPTEDKTDEIMGMRLDVARASKAYSEELAKKNPRDPQIKQL
                     MTNGRKLVTYVTRFPGAYQDEARRLLPEFAGGDAESLGNRKEPKNFLEARTAAKEAID
                     AMQAANLLIKTLPGRIATLKDPAEKAELQKQLEEAQTQVKTAQDDALFYCRLSNKFVA
                     PDTDANEVNLIRYLMCYLLYAKEDFNEAVILGEFLARRYPDSQGARPGAKIAMASYLK
                     LYTENETDDKDYETRKIISIADYIVKKWPDQPEAAEALNTLIPFMIREKKLQEAQDYL
                     AQIPAESPHRGNAELKTGQALWASYLENSQQIRDWENDPTLMPEGTDLAARKAELEPL
                     KTKAKQTLVDGVGRMQAAGDVSNVMATAVLSLVQIYVDTNEAVKAIKLMEDPKIGVLT
                     LVQNNDPSVGAPGFADETYKTALRAYISALASKEGDAADAVKKAEGIMDLLEQQMNAT
                     PQGQAKLIAIYVSLARDLQRQMEIAEPEVKKGLGQGFEAFLKQVASGATELNVLNWVA
                     ETYRGMGESFGNLRNQPKDVQDTARQYYGQAVATYQKILDMDKKDANFLSDAMATQLQ
                     LQTAKTKRSTGDYVGAMDLFESILKANSMLLPVQIEAARTYQDWGSFKDSQDNYMRAI
                     VGGRPDKNNPDPKKKNKNVIWGWGEIARLTAPTAASSQFKDQFHEARFNLALCRYNYA
                     VAQQDADKKKLNFQRAKTDIALIAGFYPDLGGEKWKAQYDALLKRIQSALQERPLGLK
                     ALETASSAASSAAGGGASTKTVPTSTPANK"
     gene            2547..3593
                     /locus_tag="VFV87_17705"
     CDS             2547..3593
                     /locus_tag="VFV87_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105662.1"
                     /translation="MLRKVALLALSALLFALSGGFDAAFGQFDQVYQRQGVPSKGNIS
                     AMSKDEVTLDMSGVARNFAVNEIARITYADEPNELNNARNAVLQQNWGQAEIELKKLD
                     GQSFARDLIRQDVEYYKALCMAKKAMTEGGDKNAAATAMLNYVKLAPNNYHFYAAAEV
                     LGDLAVSSGAFDKAATYYKPIAGAPWPDYQMRAGNAVGRALLAQKSFPEAQKQFDDVL
                     AIDLSTPEAAREKLFANVGKAQCLAETGQAEAGIALLQDIISKNDPADQVLFARTYNA
                     LGRCYLKTNKPKDARDAYLFTEMLFTADSDAHAEALYHLSKLWTDLNKSDRAVAARNT
                     LRERYSGSIWNTLE"
     gene            3919..4767
                     /locus_tag="VFV87_17710"
     CDS             3919..4767
                     /locus_tag="VFV87_17710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5105663.1"
                     /translation="MSRTSPSNGFDRGLRRQAIVESLLRDVFAGRLRAGQRLVTEDLA
                     ERYGVSHTPIREALIALAGVGIIDLVPNRGAVVREVSARDVREVCQVRRVLECEAVRR
                     ACGRIDLRALEKLAADFRELEERPEHADPKYIERARAVDSRLHDTIAASCGNQFLAQE
                     LARLKTLFRSFRDVSWAYAQARSDFRRLAGEAREHLAIVEALLAGDGKAAARAMSRHI
                     RSGVKYWTRALPAVREAGILPAIRGAGVSPAKSNGKHNSNSNGHSKTIQSNGKRPKST
                     SRSLHA"
     gene            4760..7942
                     /locus_tag="VFV87_17715"
     CDS             4760..7942
                     /locus_tag="VFV87_17715"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5105664.1"
                     /translation="MRNATKFLSFLANAALICGTTFAQEKLQYNRDIRPILAENCFAC
                     HGPDSAARKADLRLDKRDAAVEYGAIAPGKTDDSALVARIFATDEMEVMPPPATKKKL
                     TAQQKETLKRWIAEGAEYEPHWSLIPPTRPEPPAVKNAAWVKNPIDAFILAKLEAAGL
                     SPAPEADRRSLARRLSLDLTGLPPEPALVEAFVNDQDPNAYGKLVDELMSKPQWGEHR
                     GRYWLDAARYADTHGIHFDNYREIWAYRDWVIDAFNRNLPFDQFTIEQLAGDLLPNPT
                     LDQKVATGFVRCNITTNEGGIIDEEYKVLYARDRTETFGQIFLGSTVGCCVCHDHKFD
                     PFTMKDFYSLTAFFDNTTQPVRDGNIKDPPPVIPVPVDQDRERFKALQGEISGVQGQI
                     AARKQAAGSEFDAWLAGFKPEELAKYVPQEGVALRAGLNEGEGKSLKFAIGGAEREIS
                     LEDGFTWGAGRDGKGKKAFHLKAEGPAIELADVGDFEADQGFAVSCWLSLGKRGQTGA
                     VLARMDVGSDYRGWDVWLEGDKIGMHIINKWMDDALKVVCQTPLQPRQWTHVAVTYDG
                     SKKAAGVKVFYNGVPQPVSVQADALKSTIKTAVPLKIGQRSTGQRLAAMGYQDLRIYS
                     RGLSPQEVEQLAKADELEDFFAKAADQRKPEEKSKVFDWWLATVDQTSRDLDGRLKAL
                     QQEEVQIKARGTIAHIMNERNEEAMAYILFRGEYDKRRDQVKAATPEILHAMPAGAPQ
                     NRLGLAQWLFAAENPLTARVTVNRFWQELFGTGLVRSAGDFGVTGELPSHPELLDWLA
                     IEFRSPSDGSPGWDIKQFFKLLVMSNTYRQATVVTPEKLLKDAENRLLSRGPRYRMDA
                     EMIRDYALAASGLLVKKLGGPSVKPYQPDNVWEAVAMIGSNTRDYKRDTGESLYRRSM
                     YTFWKRAAPPASMEILNAPNRETCVVRRERTDTPLQALVTLNDEQFVEAARHLAQRAI
                     KEGGEKDDDRLDFVARRLLARPFRVEELPIVKASLTELSAYYTANVEDAIKLIAVGES
                     KADPALDPAQLAAWTMLVNELMNLDEVLNK"
     gene            8170..8760
                     /locus_tag="VFV87_17720"
     CDS             8170..8760
                     /locus_tag="VFV87_17720"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEX5105665.1"
                     /translation="MAKSSAIVVDQRELERKIFIVRGQRVMLDSDLAKLYAVPTKRLN
                     EQVQRNADRFPEDFAYQLTAQEFTDLKSQIATSSVGHGGKRKLPWVFTEHGVAMLSSV
                     LRSPRAVQVNIAIMRAFVRMRRLIGTPGELVTQLNELAKTVQLHDDQIAAIADVLRKL
                     MEPPSTPPKRKIGFLPPESRLSQNSPTAHSQNGARK"
BASE COUNT         1840 a         2789 c         2732 g         1428 t
ORIGIN      
        1 gaacaagctc ggcaagccga tggaagcttc ggcgatcttc aacgagctgc tcgccaaccc
       61 cgattcgccc gaggccttcc gcacgctcaa ggtcaaggtc atggccctgg cgctcgaagc
      121 ctggaaccct caaggtctat acctggaaat catcgacaaa ggaggcaagc tcgtcgattc
      181 cgcccgcccc accgaggaca agaccgacga aataatgggg atgcggctgg acgtggcccg
      241 cgcctccaag gcctattcgg aggagctggc caagaagaat ccccgcgatc cgcagatcaa
      301 gcaattgatg accaacgggc gcaaactggt cacttacgtc actcgatttc ctggcgcgta
      361 tcaggatgaa gcccgccgcc tgctgcccga gttcgccggc ggcgacgcag agagtctggg
      421 caaccgcaag gaaccgaaga actttctgga ggcccgcacg gcggccaaag aagcaatcga
      481 cgcgatgcag gcggcgaact tgctcatcaa aacgcttccc ggccggattg ccaccctgaa
      541 agaccccgcc gagaaggccg agttgcaaaa gcaacttgaa gaggctcaga cgcaggtcaa
      601 aacggcgcag gacgacgcgc tcttttactg ccgactgtcg aacaagttcg tcgcgccgga
      661 taccgacgcc aacgaagtaa atctgatccg ttacctaatg tgctatctgc tctatgcgaa
      721 ggaagacttc aacgaagcag tcatcctggg cgagttcctg gcccgccgct atcccgacag
      781 ccaaggcgct cggcccggcg cgaaaatcgc catggcctcc tatctcaagc tctatacgga
      841 gaacgagacc gacgacaagg attacgagac ccggaagatc atttcgatcg ccgactacat
      901 cgtcaagaaa tggcccgacc agcccgaagc ggctgaagcg ctgaacacgc tgatcccctt
      961 catgatccgc gagaagaagc tgcaggaggc gcaggattac ctcgcgcaga ttcccgcgga
     1021 atcgccccac cgcggcaacg cggagctgaa gaccggccag gccctgtggg ccagctattt
     1081 ggagaactcg cagcagattc gcgattggga aaacgacccc acgctgatgc ccgaggggac
     1141 cgacctggcc gcccgcaagg cggaattgga gcccctcaag accaaggcca agcagacgct
     1201 ggtcgatggc gtcggccgca tgcaggctgc cggcgatgtg agcaacgtga tggcgaccgc
     1261 cgtcctctcg ctcgtgcaga tttacgtcga tacgaacgag gccgtgaagg cgatcaagct
     1321 tatggaggat ccgaagatcg gcgtcctgac gctcgtgcag aacaacgacc cgtcggtcgg
     1381 cgctccgggc tttgccgacg aaacgtacaa gaccgccctg cgggcctaca tctcggccct
     1441 ggcttccaaa gaaggcgatg cggctgatgc ggtcaaaaag gccgaaggga ttatggattt
     1501 gctcgaacag cagatgaacg cgacgccgca agggcaggcc aagctgattg ccatctatgt
     1561 cagcctggcc cgcgacctgc agcggcagat ggaaatcgcc gagccggagg tgaagaaggg
     1621 cctcggccag ggcttcgagg cgtttctgaa gcaggtcgcc tccggcgcga ccgagctgaa
     1681 tgtcctcaat tgggtcgccg agacctaccg cggcatgggc gagagcttcg gcaatctccg
     1741 caaccagccg aaggacgtgc aggacacggc caggcagtac tacggccagg cggtcgccac
     1801 gtaccagaag attctcgata tggacaagaa ggacgccaac ttcctttcgg acgcgatggc
     1861 cacgcagttg caactgcaga cggccaagac caagcggagc acgggggatt atgtcggcgc
     1921 gatggacctg ttcgagtcga tcctcaaggc caattcgatg ctactaccgg tgcagatcga
     1981 ggcggcccgg acctatcagg attgggggag cttcaaggac tcgcaggaca actacatgcg
     2041 ggcgatcgtc ggcggccggc cggacaagaa caaccctgac cccaagaaga agaacaagaa
     2101 cgtcatctgg ggctggggcg aaattgctcg cctgaccgcg cccaccgccg ccagcagcca
     2161 gttcaaggat cagtttcacg aagcccggtt caacctggca ctctgccgtt acaactacgc
     2221 cgtggcccaa caggacgcgg acaagaagaa gctgaacttc cagcgggcca agaccgacat
     2281 cgcgctgatc gccgggttct atcccgacct gggcggcgag aaatggaagg cccaatacga
     2341 cgcgctgctc aagcggatcc agtcggcgct gcaggagcgg ccgctggggc tcaaggcgct
     2401 ggaaaccgct tcctctgctg catccagtgc cgcagggggc ggggcctcga ccaagaccgt
     2461 gccgaccagc acgcccgcca acaaatagac ctcctttcct atccctgaac cgaacacata
     2521 cgccagccat tgcttgagga tcaaacatgc ttcgcaaagt cgccctgctc gctctttcgg
     2581 ctctgttgtt cgcgctcagc ggcggattcg acgccgcctt cggccagttc gaccaggtct
     2641 atcagcgcca gggcgtgccc agcaagggca acatctcggc gatgagcaag gacgaggtga
     2701 cgctcgacat gagtggcgtg gcccgcaact tcgcggtcaa cgagatcgca cggattacgt
     2761 acgccgacga gcccaacgag ttgaataacg cccgcaacgc cgtcctgcag cagaactggg
     2821 ggcaggcgga aatcgagctg aaaaagctcg acgggcagtc cttcgcgcgc gacctgatcc
     2881 gccaggatgt cgagtattac aaggccctgt gcatggccaa gaaggcgatg accgaagggg
     2941 gcgataagaa cgccgcggct acggccatgc tgaactacgt caagctggcc ccgaataact
     3001 atcacttcta tgctgccgcg gaagtgctcg gcgacctggc cgtctcgtcc ggcgcgttcg
     3061 acaaggccgc cacctactac aagccgatcg ccggcgcccc ctggcccgat taccagatgc
     3121 gggccggcaa cgccgtcggc cgggccttgt tagcccagaa gagttttcct gaagcgcaga
     3181 agcaattcga tgacgtcctg gcgatcgacc tttccacccc cgaagccgcc cgcgaaaagc
     3241 tcttcgccaa cgtcggcaag gcccagtgcc tggccgaaac aggccaggcc gaagcgggca
     3301 tcgccttgtt gcaggacatc atcagcaaga acgatccggc tgatcaggtg ctgttcgccc
     3361 ggacgtacaa cgccctgggg cggtgctacc tgaagacgaa caagcccaag gatgcccgcg
     3421 acgcctattt gttcaccgag atgctcttta cagccgattc cgacgcccat gccgaagcac
     3481 tctatcacct gagcaagctg tggaccgacc tcaacaagtc ggaccgggcc gtcgccgccc
     3541 gcaacacgct ccgggagcgc tattccggaa gcatctggaa cacgctggag taaaaccgca
     3601 ggcttctcgc tctgtgagaa gcggacgact ctccatttgg agggcctccg cgccccgcgc
     3661 gagccggccc tggcggcgcg cggagtcgtt ccgccaaaac acccgtactg ccgctcgcgg
     3721 agcgagcggc ctacggtggg cggagcccac cctgcgtttt cgcgcgcggg gcttgcattg
     3781 cggcctggga ttctatagaa agaatactag gttctgcaga actacggcga accagaacct
     3841 cggcgacgga gcgccgcccg cccataatca tcctgccttc cgccttgtcc gccgctttga
     3901 tggacaggcc tgcctgccgt gtctcgaact tctcccagca acggctttga ccgcggcttg
     3961 cgccgccagg cgatcgtgga gtcgctcctg cgcgacgtct tcgccggccg cttgcgggcc
     4021 ggacagcgcc tcgtgacgga ggacctggcc gagcgctacg gcgtcagcca cacgcccatc
     4081 cgcgaggccc tgatcgccct ggccggcgtc ggcatcatcg atctggtgcc caatcgcggg
     4141 gcggttgtcc gcgaggtctc ggcccgcgac gtccgcgagg tctgccaggt ccgccgagtc
     4201 ctcgaatgcg aagccgtccg ccgcgcctgc ggccggatcg acctgcgggc gctcgaaaag
     4261 ctcgccgctg acttccggga gctcgaagag cggcccgagc acgccgaccc gaaatacatc
     4321 gagcgggccc gcgccgtcga tagccggctg cacgatacga tcgccgcttc gtgcggcaac
     4381 cagtttctgg cccaggagct ggcgcggctc aagactttgt tccgctcgtt ccgcgatgta
     4441 tcgtgggctt acgcccaggc ccgcagcgac ttccgccgcc tggcgggcga ggcccgcgag
     4501 cacctggcga tcgtcgaggc gctgttggcc ggtgacggca aggctgcagc ccgggcaatg
     4561 tcgcggcaca tccgctccgg cgtgaaatac tggacgcggg ccctccccgc cgtccgtgag
     4621 gcaggcatct tgcctgccat acgtggggcg ggcgtctcgc ctgccaagag caacggcaag
     4681 cataactcga acagcaatgg ccactccaag accatccaat ccaacggcaa acgaccgaaa
     4741 agcacgtcga ggtctctcca tgcgtaacgc gacgaagttt ctctccttcc tggcgaatgc
     4801 ggctttgatc tgcggaacaa cattcgcgca ggaaaagctg cagtacaacc gcgacatccg
     4861 cccgatcctg gcagagaact gcttcgcctg ccacggcccg gacagtgcgg cccgcaaggc
     4921 cgacctgcgg ctggataagc gcgatgcggc agtcgaatac ggagcgattg cgccgggcaa
     4981 gaccgacgat agcgccctgg tcgcccgcat tttcgcgacc gacgagatgg aggtgatgcc
     5041 gccccccgca acgaagaaga agctcaccgc ccagcaaaaa gagacgctga agcgctggat
     5101 cgccgaggga gccgagtacg agccgcactg gtcgctgatt ccgccgacgc gtcccgagcc
     5161 acccgccgtg aaaaacgccg cctgggtcaa gaatccgatc gacgccttca ttctggccaa
     5221 attggaagcc gccggcctct cacccgcccc ggaagccgat cgccgttcgc tggctcgccg
     5281 tctgtcgctg gacctgacgg gcctgccgcc ggagccggcg ctggtggagg cgttcgtcaa
     5341 cgatcaggat ccgaacgcct acggcaaact cgtcgatgaa ttgatgtcga agccgcagtg
     5401 gggcgagcat cggggccgct attggctcga cgccgcccgc tatgccgaca cgcacggcat
     5461 tcatttcgac aactaccgcg aaatctgggc ctatcgcgat tgggtgatcg acgccttcaa
     5521 ccgcaatttg ccgttcgacc agttcacgat cgagcagctt gccggcgatc tcttgccgaa
     5581 tccgaccctc gatcagaagg tcgccacggg cttcgtccgc tgcaacatca cgacgaacga
     5641 ggggggcatt atcgacgagg aatacaaggt gctctatgcc cgcgatcgga cggaaacgtt
     5701 cgggcagatc ttcttggggt caactgtcgg ttgctgcgtc tgccacgatc acaagtttga
     5761 tcccttcaca atgaaggatt tttattcgct gacggcgttc ttcgacaaca cgacgcagcc
     5821 ggtgcgggac ggcaacatta aggacccgcc gccggtgatt ccggtgccgg tcgatcagga
     5881 ccgcgagcgg ttcaaggcgc tgcaaggcga aatcagcggc gtgcaaggtc agatcgccgc
     5941 ccgcaagcag gctgccggca gcgaattcga cgcctggctg gcggggttca agcccgaaga
     6001 gctcgcgaaa tacgttcccc aggaaggggt cgcgctgaga gccggactta acgaagggga
     6061 gggaaagtcg ctcaagttcg cgatcggcgg cgcggagcgc gagatctcgc tggaagacgg
     6121 cttcacgtgg ggtgcgggcc gcgacggcaa ggggaagaag gccttccatc tcaaggcgga
     6181 ggggccggcg atcgaattgg ccgatgtcgg cgatttcgaa gccgatcagg gctttgccgt
     6241 gagctgctgg ctctcgctgg ggaagcgcgg gcagaccggc gcggtcctcg cgcggatgga
     6301 tgtcggcagc gattaccgcg gctgggatgt ctggctggaa ggcgacaaga tcggcatgca
     6361 catcatcaac aagtggatgg acgacgcctt gaaggtcgtc tgccagacgc cgctgcagcc
     6421 gcggcagtgg acgcacgtgg ccgtaaccta cgacggctcg aagaaagccg cgggcgtcaa
     6481 ggtcttttac aacggcgtcc cgcagccggt gagcgtgcag gccgatgcgc tgaagagcac
     6541 aatcaagacc gcggtgccgc tgaagatcgg ccagcggagc accggccagc gactggcggc
     6601 gatgggctac caggatctgc ggatctacag ccgcggcctt tcgccgcagg aggtcgagca
     6661 gctcgccaag gccgacgagc tggaggactt cttcgccaaa gcagccgacc agcgcaagcc
     6721 agaggaaaag agcaaggttt tcgactggtg gctggccacg gtcgatcaaa ccagccgcga
     6781 tctggacggc aggctcaaag cgctgcagca ggaggaggtg cagatcaagg cccgcggcac
     6841 aatcgcccac atcatgaatg agcggaacga agaggcaatg gcctacatct tgttccgcgg
     6901 cgaatacgac aagcgccgcg atcaggtcaa agcggccacg cccgagattc tgcacgccat
     6961 gcccgcaggc gcgccgcaga accgattggg cctggcccaa tggctgttcg ctgccgagaa
     7021 cccgctgacc gcccgcgtga cggtcaatcg cttctggcag gagctattcg gaaccgggct
     7081 ggtccgctcg gcgggcgact ttggggtcac gggcgaattg ccctcgcatc ccgagctgct
     7141 cgactggctg gcgatcgaat tccgctcgcc gtcggacggc tcgcccggct gggacatcaa
     7201 gcagttcttc aagctgctgg tgatgagcaa tacctaccgg caggcgacgg tggtgacgcc
     7261 cgagaagctg ctgaaggatg ccgagaaccg cctgctttcg cgcggcccgc gctaccgcat
     7321 ggatgccgaa atgatccgcg actatgccct ggccgccagc ggcctgttgg tgaagaagct
     7381 cggcggtccc agcgtcaagc cgtatcagcc ggacaacgtt tgggaggcgg tggcgatgat
     7441 cggcagcaac acccgcgatt acaagcggga cacgggagag agcctttatc gccggagcat
     7501 gtacacgttc tggaagcggg cggccccgcc ggcttcgatg gaaattctta acgctcccaa
     7561 ccgcgaaacg tgcgtcgtcc gccgcgagcg gaccgacacg ccgctgcagg ccctggtgac
     7621 gctgaacgac gagcagttcg tcgaggcggc ccggcatttg gcgcagcggg ccatcaagga
     7681 aggtggcgaa aaggacgacg atcgattgga cttcgtcgcc cgccgcctcc ttgcccgccc
     7741 gttccgagtg gaggagctgc ccattgtaaa agcgtcgctc accgagctgt ccgcctatta
     7801 cacggccaac gtcgaagatg ccataaagct catcgccgtg ggcgaatcca aagccgaccc
     7861 ggccctcgat cccgcgcagc tcgccgcctg gacgatgctg gtcaatgagc tgatgaacct
     7921 ggatgaggta ctgaataaat gaacgtagca acccgcctcg cgcgcaagcg ccgcgtgatt
     7981 gcgccggcca gcgctccgct tcctcagcca gacgccctgc tggccgtgac ggtggacgat
     8041 gacgaggatg ttgagtggat ttggacctac catgctgatg gcagcaggac ggttagcggt
     8101 taccacattc gactgcgctt gcaggattta gagtcgattc tgaaatcaag ctcgtgcagc
     8161 aggtgattca tggcgaagag ttcggcaatc gtcgtcgacc agcgcgagtt ggagcggaag
     8221 attttcattg tccgcggcca gcgtgtcatg ctggattccg atttggcgaa gctctacgca
     8281 gtgccaacga agcgtcttaa cgagcaagtt cagcgaaacg cagatcgctt tcctgaggat
     8341 ttcgcgtatc agcttacggc gcaagagttt acggacttga agtcgcaaat tgcgacttca
     8401 agtgtggggc acggtggcaa gcgaaaactc ccctgggtct tcaccgagca tggtgtcgcc
     8461 atgctctcca gcgtcctccg ctccccgcga gccgtgcagg tcaatatcgc cattatgcga
     8521 gctttcgtcc gcatgcggcg gctgattggg acgccgggtg agttggtcac gcaactgaac
     8581 gagttggcta agaccgtcca gcttcacgac gaccagattg cggccatcgc cgacgtgttg
     8641 cgcaagctga tggagccgcc gtcgactccg cccaaacgca agattggctt cctgccgccc
     8701 gagtcccgcc tctctcaaaa ctctcccacc gcacattctc agaacggagc cagaaaatga
     8761 tttcctacaa cgaattcaag cagaacatc
//
LOCUS       DASUGB010000932         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1909916, whole genome shotgun sequence.
ACCESSION   DASUGB010000932 DASUGB010000000
VERSION     DASUGB010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909916"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFV87_17725"
     CDS             complement(<1..369)
                     /locus_tag="VFV87_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105666.1"
                     /translation="MPEVRGTRGFKKLMVSVACPTVSMIPLSRSLLTAWVCVGVVLWG
                     SPALGQDRAQDNPTRFEIGAGFATLWQHSESGAFARSPGLSVSFGVPLARHLALETEL
                     AWFPGSAPGGFKDQGGQALQF"
     gene            complement(531..1382)
                     /locus_tag="VFV87_17730"
     CDS             complement(531..1382)
                     /locus_tag="VFV87_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105667.1"
                     /translation="MTAHVRRRLKLVAALAFVGTAACIPTSSFNGNWRIQTRQSVWPF
                     PQFAARNVWVIGNWVADQAGAVGSATVFDNYPGTTENGLSVCLNCRANATWYFVFHSG
                     PCNTESAGTHVMGYQQTVQLDCTSFNNEWFAYQSNSSGYLATSVPEEWESGSAEPPEA
                     GGPGTLIPAEGRDSDTALQPDGDGIDSPSGQYHLQYQGDGNLVLYDTWNSWTAVWATM
                     TNDNNPGAVVMQGDGNLVIYNGDSEAVWATGTDGNSNAYLAVTDAGMLVILREDGFAL
                     WWTPPPS"
     gene            complement(1415..2158)
                     /locus_tag="VFV87_17735"
     CDS             complement(1415..2158)
                     /locus_tag="VFV87_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105668.1"
                     /translation="MLVVFFVQGLIAQGAATLLAQKSVAPHENAFQSLNREAAAARKG
                     EQAAIHTVVQEIFDTFSPFDVPVSSPLRSRIERAEKAYRVGAHPPVKAGALVDGINEM
                     VAALGAPGWLRTSSEQVQLFRSGLKPVVPQLVGQKPSGKGMFGISDEMSPAEAAFIGL
                     YLMSAKMWTDGYRVPPEDWVRMMKTERVFLQESGPASMTAPAARATVQRPPKFVSDLE
                     SFVSAGLRDGNSPTTARAGQFLDRLGIAR"
     gene            complement(2561..2887)
                     /locus_tag="VFV87_17740"
     CDS             complement(2561..2887)
                     /locus_tag="VFV87_17740"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5105669.1"
                     /translation="MSYDYQAIAADVKAALEADPKISLLSICKDRQIDRHTINRALRG
                     CLGVVFRELKARVQAEQIQDVLQKAKLKPIKQAASDAGYGSSSALAKHARRMLGRPPT
                     AVRRGG"
     gene            complement(3143..>3395)
                     /locus_tag="VFV87_17745"
     CDS             complement(3143..>3395)
                     /locus_tag="VFV87_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105670.1"
                     /translation="ACVRHIAVRHSLRSPSWEWPMRHRVSFLSVHHARRLSTLALIVA
                     GTAVASSHAPFSPGSGVFGVVSARPERGHFSTPLDRWTW"
BASE COUNT          678 a         1062 c         1019 g          636 t
ORIGIN      
        1 aaactgcagc gcttgtccgc cttgatcttt gaagccaccg ggtgccgatc ccggaaacca
       61 ggccagctcg gtctcaaggg ccagatgacg cgcgagcggg acgccaaacg aaacggacaa
      121 cccaggcgac cgagcaaacg cacccgactc gctgtgttgc cacagcgtcg cgaatcctgc
      181 accgatttcg aagcgtgtgg gattgtcctg agcgcgatcc tggcccaggg ccggactgcc
      241 ccaaagaacg acacccacac agacccaagc ggtcaggagc gagcgggata gagggatcat
      301 cgagacagtt ggacatgcaa ccgacaccat gagttttttg aaaccacggg tgccgcgaac
      361 ctcaggcact cgccttctgc tggcgtgacc tccggtttgg ttgacgtcgc ggtaaagggg
      421 gtgccgcacg cgacaatatt gcacacaatc gtacacggtg ggcgaatcca aacccggcgg
      481 tgagccgctt ctcgggattc ccgaggagcg actcacggcc ggcgcagtct ctaggacgga
      541 ggcggcgtcc accagagcgc aaacccgtcc tcgcgaagga tgaccagcat gcccgcgtcc
      601 gtcaccgcga gataggcatt cgaattgccg tcggtgcctg tcgcccagac cgcttcgctg
      661 tcgccgttat agatcaccaa attcccatcg ccctgcatca ccacggcgcc gggattattg
      721 tcgttcgtca tcgtggccca cacggcggtc cagctgttcc aggtgtcata cagcactaaa
      781 ttgccgtcgc cctggtactg caagtgatac tggccgctgg gagagtcgat gccatcgccg
      841 tccggctgga gagcggtgtc ggaatctcgt ccttcggctg ggatgagggt gccaggcccg
      901 ccggcctctg gcggttctgc agacccggac tcccactcct cggggaccga cgtggccaga
      961 tacccactgg aatttgactg atacgcgaac cattcgttat tgaaagaggt gcagtcgagc
     1021 tgaacggtct gctggtatcc catcacgtgc gttccagcgg actccgtatt gcaggggccc
     1081 gaatggaaga cgaagtacca cgtggcgttc gctcggcagt tcaaacagac tgaaagcccg
     1141 ttttccgtgg tgccaggata gttgtcaaac acggtggcgc ttccaactgc gccagcctgg
     1201 tcggccaccc aattaccgat cacccagaca tttctcgcgg caaactgcgg gaagggccag
     1261 accgattgcc gcgtttggat tcgccagttg ccgttgaaac tgctggtggg aatgcacgcg
     1321 gccgtgccca caaaagcaag agccgccact aacttgagcc ttcgtcgcac gtgtgctgtc
     1381 atgctttgac tccctgtctt ggtgtgacgc gttgctaccg ggcgatgccc aatcgatcta
     1441 ggaattgccc tgcccgcgcc gtcgtgggac tgtttccgtc gcggagtcct gcggagacga
     1501 aactctccaa atcagacaca aacttcggag gcctctgcac cgtcgcccta gccgctggcg
     1561 ccgtcatgct ggctgggccg ctctcctgca gaaacacacg ctcggtcttc atcatgcgca
     1621 cccagtcctc gggtggcacg cgatacccgt cggtccacat cttggcgctc atgaggtaca
     1681 ggccaatgaa cgccgcttcg gccggcgaca tctcatcaga gatcccgaac atgcctttgc
     1741 cggacggctt ttggccgacg agctgcggca ccacaggctt gaggccactt cgaaacagct
     1801 ggacttgttc cgagctggtt ctcaaccaac cgggcgcacc gagcgcggca accatctcat
     1861 tgattccgtc aaccagtgcc cccgccttga caggcggatg tgccccaacc cgatacgcct
     1921 tctcggcccg ttcgatccga gatcgcagag gactcgacac cggaacatca aagggcgaaa
     1981 acgtgtcgaa gatttcctgg acgaccgtat gaatcgccgc ttgttcgcct ttgcgggcgg
     2041 cggcagcctc gcggttcaag gattggaagg cattctcgtg aggggcgacg gatttctgag
     2101 cgagtagcgt agcggccccc tgtgcaatga gcccttggac gaagaacacg acaagcacgc
     2161 tggttccgcg gcccaggccc ggtcttcgac gcgtcatatt cacctcctga aagtacgact
     2221 actcgatcgc gcgatccatc acgatcgctc ctgctgcgtc ctcggacact tgtgagccgc
     2281 tgcggtcgga ataggtgtcc gcggcggtgt cctcgtcctg gttcgaagag attcatagat
     2341 caacaccgct gccgcgacaa tcatcgccag agacgcgaga aggagcgtct gtcgtcgcgc
     2401 cgcttccgag cattgtcagc acctcaagga acgcgagcgg ctcaatcaca accgagcgcg
     2461 agcacgctcg gtcgggccaa attcttcgtc agcccgacta gagacaacaa gggccaaatg
     2521 cggcccgttt ggtgcattcg ttgtccagtt ggagcaagtc tcaacctcct cgcctgacag
     2581 ccgtcggcgg ccggccgagc atccggcgag catgcttggc cagtgccgaa ctcgatccgt
     2641 agccagcatc ggacgctgcc tgcttgatcg gtttcagttt ggctttctgc agaacatcct
     2701 gaatttgttc ggcctgcacg cgagctttga gctcccgaaa aaccacgcca aggcagccgc
     2761 gtaacgcgcg attgattgtg tgtcgatcga tttgtcggtc cttgcagatg gagagcaggc
     2821 taatcttcgg atccgcctca agcgccgcct tcacgtctgc cgcgattgcc tggtagtcat
     2881 agctcatggc gcaccgaact tccgatgacc agtggctcgg tggtgatgaa ctgaagttgc
     2941 cctggtttcg gtgtgcagcg ccagcgtcga tacgtccgac ataaacagac ccggggccgc
     3001 tgaggtgggc atgatggcgg ccagcggcgc gccggtaagc ccaaaggtcc ggtttggtgg
     3061 ttgtagctgc gcgtgatggc caggcccatg ccggcgttgc ttggcagcac gagatcggcg
     3121 aacgtcatga ccaggttgcc gttcaccatg tccaccgatc cagtggcgtc gagaagtggc
     3181 cgcgctcagg acgtgcgctg acgacgccaa acacgccgct gccggggctg aacggcgcgt
     3241 gtgaactcgc gacagcggtg cccgcaacaa tcaacgcgag cgtgctcagg cgacgagcat
     3301 ggtggacgga aagaaacgac acacgatggc gcatcggcca ctcccaggaa ggcgaacgca
     3361 gagagtgtcg gaccgcgatg tgacgaacgc aagcc
//
LOCUS       DASUGB010000933         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_382116, whole genome shotgun sequence.
ACCESSION   DASUGB010000933 DASUGB010000000
VERSION     DASUGB010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382116"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFV87_17750"
     CDS             <1..662
                     /locus_tag="VFV87_17750"
                     /inference="COORDINATES: protein motif:HMM:NF044858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105671.1"
                     /translation="TPEARSTEEFALSPAGALAAAIGRAVSSRGQMSPRQYFRVVLEL
                     LERGPCNLLVVGAGRDTELYVRANAGGRTVVLERHARWIDEVRHLDCEVVQVAYTTRI
                     ADGPQEPCSLPEGLPAGLLDEKWDVILIDGPEGNRGESPGRQQSVLLARELAHTGTTV
                     FLHDYDRPAERAVASRYLKAADERHGGSPALAVFQYRNTAEGAPAPDMRADLYGGIRC
                     R"
     gene            775..1650
                     /locus_tag="VFV87_17755"
     CDS             775..1650
                     /locus_tag="VFV87_17755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5105672.1"
                     /translation="MALLLGVQIILYVLPHLRPGPVGWLCWMIGSDGLLWLGLAGIFL
                     LVALAFSLWRRPLLNRWRVLGLAAIFALAGSTFMFRVYPSSHDGTPSAVHFRLPLDGP
                     ILVGWGGDTPEENYHVAYPDQRYAYDLLVARDGSTHQGEGRELADYYCYGLPVLAPAD
                     GKVIAVLDGRPDQPIAVLGGEPAGGNQVVIEVAPREFLFLCHLSRGSILVKEGDPVSV
                     GQPLAKVGNSGNTSEPHLHIHLQDTPRLHVGEGIPLLFWGYRADGRWIDRGMPTGGFS
                     LDGPIGQTVEHEAGR"
BASE COUNT          292 a          632 c          699 g          381 t
ORIGIN      
        1 agacgccaga agccaggtca acggaggaat tcgccctttc gccggctggg gcgctggcgg
       61 cggcgattgg gcgggcggtg tcttcgcggg ggcagatgtc gccgcggcag tattttcgcg
      121 tagtgctgga attgctggag cgcggtccgt gcaacttgct ggtggtcggc gccggccgcg
      181 ataccgagct ttatgtgcgg gccaatgccg gcgggcggac ggtagtgctt gaacgccacg
      241 ccaggtggat cgacgaggtg cggcacttgg actgcgaggt ggtgcaggtc gcttatacga
      301 cccgcattgc ggacgggcca caggagccgt gttcgctgcc ggaaggcctg ccggcgggct
      361 tgctggacga gaagtgggac gtgatcctga tcgacggtcc cgaaggaaac cggggcgaaa
      421 gtcccggccg ccagcagagc gtgctcttgg cgcgcgagct cgcccacacc ggtacgacgg
      481 tctttctgca cgactacgac cgccccgccg agcgggcggt agccagtcgg tatttgaagg
      541 ccgccgacga gcggcatggc ggctcaccgg ccctggcggt attccagtac cgcaacactg
      601 cggagggagc gccggccccg gatatgcgcg ctgaccttta cggcggcatc cgctgtcgct
      661 agtaaatcgg ttgtggaaaa aacttccggt cccgcaatca acggtcaggc gacttcctcc
      721 ggcctcccgc aaccgagcag gcgttcgccg cggccggatt ggtggctata tggcgtggcg
      781 ctgttgctgg gcgtgcagat tattctgtac gtgctgccgc atctgcggcc tgggccggtg
      841 ggctggctgt gctggatgat cggcagcgac ggcttgttgt ggctgggcct ggcgggaata
      901 tttctgctcg tggctctggc gttctcgctg tggcggcggc cgctcttaaa ccgctggcgg
      961 gtgctcgggc tggcggccat cttcgccctg gccggctcga cgttcatgtt ccgcgtctat
     1021 ccgtcgtcgc acgacgggac gccgagcgcg gtccactttc gcctgccgct cgacgggcca
     1081 attcttgtgg gctggggagg cgacacgccg gaggaaaatt accacgtcgc ttatcccgac
     1141 cagcggtatg cgtatgactt gcttgtggcc cgcgacggca gcacccacca gggtgaaggc
     1201 cgcgagctgg ccgactatta ctgctacggc ctgccggtgc tggctccggc cgacgggaag
     1261 gtcatcgcgg tgctggacgg ccggcccgac cagcccatcg ccgttctcgg cggcgagccg
     1321 gccggcggga atcaggtcgt catcgaagtg gcgccgcgcg agtttctctt cctctgccac
     1381 ctgagccgcg gctcgattct agtcaaggaa ggggacccgg tctccgtcgg ccagccgctg
     1441 gccaaggtcg gcaattccgg caacaccagc gagccccacc tgcacattca tttgcaggac
     1501 accccgcggc tgcacgtagg cgaaggaatt ccgctcctct tttggggcta tcgggcggac
     1561 gggcggtgga tcgaccgcgg catgccgacc ggtggcttct cactcgacgg gccgattggc
     1621 cagacggtcg agcacgaggc gggccgctga aaaatcgcgg ctgagtcgcc cgcctcagcc
     1681 gcggcgtatc cgaggtctgg tccgcggaaa ctgccgattt tccgggcagt tttccgggtg
     1741 caaccccgct cgcggctagt taagatggaa ttggtcgtac ggctaccctc gttggccctt
     1801 atcggccacg tgcgatcgct tctatcgctt agcgagtctg ccatgattcg tcgcttgctt
     1861 tttgcgcttg gcattgttat tgccctcact ctggccgccc tcccgccgac ggcccaggcc
     1921 caggtaagcg cgacgggcct gatcgagcct gccgccgccc ggcaactggg cctggagcgg
     1981 atgtggttca cgcagatcgg cctg
//
LOCUS       DASUGB010000934         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_938858, whole genome shotgun sequence.
ACCESSION   DASUGB010000934 DASUGB010000000
VERSION     DASUGB010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938858"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VFV87_17760"
     CDS             complement(<1..1502)
                     /locus_tag="VFV87_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105673.1"
                     /translation="MDDGTIRTTIDPSVQPPPVSGVAEPPPLEPPVSSPPPIAAPPIA
                     AEPPIASQPPIAAEPPAIDAQPQPVTLAGLIRSAVPWATSLCVNLVVVLVLALLVLPL
                     LVKEQTVLDITFHVLDPEQQTEQVQTLTITSWEHAPDTMTEQVQQLAAVLEQQVEIQP
                     DQPVEVATEVAAALVDPTGQVPLLPSRETLNTPVSIGATAAPPTEKELNEAGSVEEAV
                     DRLGTQISEMLGQGDLLVVWLFDQSLSLVDDRQRVAARLETVYSQIEQEQQTIASRTG
                     GKPPLLTNAVVAYGSGAGEIVGPTSRAGRAIEAIRNLPVDPSGLENVFSAVALAARRY
                     GGSVKRQLLLVVWTDESGDDILLLEPTIALCKKEQVVVSAIGPTAVLGAEEGTHSYVH
                     PPNGQLYFLPVKKGPDAAFPERLRWPYWWSTSLPAWHNDIGTRARFEGWYGGRQLAAM
                     SSGFPPYALTRLTRETGGSLTIFDRGLDSGPFKLSVMRPYAPDYRSAAEIL"
     gene            2409..>2840
                     /locus_tag="VFV87_17765"
     CDS             2409..>2840
                     /locus_tag="VFV87_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105674.1"
                     /translation="MLRWEGRREKTWKVARCAATSPCALRRAFDHPFADDCPQAVLFG
                     EFVPGVSWSVCELGRHKLQIPFRAGEVALDIANSGVQHLAKTGARSQLADQSSAGLAA
                     HHVVAVAVPTERSQGEHIQRDVHVELPSELKFPWDNWMLMGC"
BASE COUNT          502 a          868 c         1003 g          467 t
ORIGIN      
        1 aggatctccg cagccgagcg gtagtcgggc gcatagggcc gcatgaccga gagcttgaac
       61 gggccgctat cgaggccgcg gtcgaagatg gtgagtgagc cgcccgtttc gcgcgtcagc
      121 cgcgtgaggg catagggcgg aaagccggac gacatcgcgg cgagctgccg gccgccgtac
      181 cagccctcga agcgggcccg cgtgccgatg tcgttgtgcc aggcggggag gctggtgctc
      241 caccagtacg gccagcggag gcgctccggg aacgcggcat cggggccttt cttcaccggc
      301 aggaaataga gctggccgtt cggcggatgg acgtacgagt gcgtcccttc ttccgcaccc
      361 agcaccgcgg tcgggccgat cgccgagacg acgacctgct ctttcttgca gagggcgatc
      421 gtcggctcca ggaggagaat gtcgtcgccc gattcgtcgg tccagacgac gagcagcaat
      481 tgccgcttga ccgagccgcc gtagcggcgg gccgccaggg cgacggcgga gaaaacgttc
      541 tccaggccgc tgggatcgac cggcaaattg cgaatcgcct cgatcgcccg accggcgcgg
      601 gaagtcggcc cgacgatctc gcccgccccg ctgccgtaag caacgaccgc gttggtcagg
      661 agcggcggct tgccgccggt gcgcgaggcg atcgtctgct gctcctgctc gatctgggaa
      721 tagacggttt cgaggcgcgc ggcgacgcgc tggcggtcat cgaccaggct cagcgactgg
      781 tcgaacagcc agacgacgag cagatctccc tggcccagca tctcgctgat ttgcgtgccg
      841 agtcgatcga ccgcttcctc gacgctgccc gcctcattca gctctttctc ggtcggcggg
      901 gcggcagtgg cgccgatcga aaccggcgtg ttcagcgtct cgcggctggg gaggagcggg
      961 acctggcccg tgggatcgac cagcgcggcg gcgacttccg tggccacctc caccggctga
     1021 tcgggctgga tctcgacctg ttgctcgagc accgccgcga gctgctggac ctgctcggtc
     1081 atcgtgtcgg gcgcgtgctc ccaggaggtg atcgtcagcg tctgcacctg ctcggtttgc
     1141 tgttccggat cgagaacgtg aaacgtgatg tccaggacgg tctgttcttt caccagcagc
     1201 ggcaggacca agagcgccag gaccaggacc acgacgaggt tcacacacag cgacgtcgcc
     1261 cacggaacgg cggagcgaat caggcccgcc agcgtgacgg gctgcggctg ggcatcgatc
     1321 gccggcggct ctgcagcgat tggcggctgc gaagcgatgg gtggttccgc tgcaatcggc
     1381 ggcgcggcga tcggaggcgg actggaaacc ggcggctcaa gtggcggcgg ctcggcgacg
     1441 ccactcacgg gaggcggttg cacgctggga tcaatcgtgg tgcggattgt gccgtcgtcc
     1501 ataccaaggc cacgcgaagg acaactgccg ccaggaacgg cccattatca acgccctcag
     1561 gcgcgcgggt caagcgcgac ggggagacgg cgtccagccc ggcgacaact tccaggtcgt
     1621 cgatggctgg atcgaatcga aggcggtcga ggacgacgtc gtgacgggcg gcgagcgcta
     1681 ccagcgctac cgcgttgtcg ccaagatcag caagtcgagc gcctcggcgc cgagcgcctc
     1741 tcacgccggt tcgatcagct ctcgttcgcc cccttcgcgc tcggctacgt cggccttaac
     1801 gctgcgctgc cgctggtggc agggtaggcg agcgtcgcgc caaaatggcg atgcagttct
     1861 ccacggtgct tcatggctgg aaggaagaac ggtcaaacga cgcactgccg aaatggacca
     1921 gacttgtcct tttcaccgag gcggtcaaaa aaacttaggg tgcggggcgg gggcgcaagg
     1981 cgttggtgga caggagttta taagcaggcg ggtcgtcgca agtcggcgcg gggcaaaaaa
     2041 accggcagaa ttcgtttgga gcgcagggag gcgctgtgta aactctcgga caatagtgta
     2101 catgtaatta cttagcgccc agcgtttggc ctgtcggccg ggtgacccgc cgcgaccacg
     2161 gcgtggagat cgactcgcgc cggcggagct ccctcgctca ggctgcgcgc ttgggtgcgg
     2221 cagggagagc gcagtgtgag gcgccgcggc gaaattttcg gggggcaatg cggcacgaat
     2281 tgggtggtga aggcgaggaa gaaatgaaaa cgacatcgag aaagtgctgg acaacgcgca
     2341 acagcgcaac atcctcatgt ggggggtgtt gcgcggcgca agtgaatgct gggatgggag
     2401 ttaggaggat gctgcgctgg gaaggtaggc gcgaaaaaac gtggaaagtt gcgcgctgcg
     2461 cggccacctc accatgcgcg ctgcgccgcg cattcgacca tccattcgcg gacgattgcc
     2521 ctcaagccgt gctgtttggt gagtttgttc ccggagtttc ctggtcggtc tgcgaactcg
     2581 gacgccacaa gctccagatc ccgtttcgcg ccggcgaggt cgccctggac atcgcgaatt
     2641 cgggcgtgca gcaccttgcg aaaaccggcg cccggtccca gctcgcggat cagagcagtg
     2701 ccggtctggc cgcccaccat gtggtcgccg tagccgtccc caccgagcga tcgcaaggcg
     2761 agcacattca gcgcgacgtt cacgtagaac tgccttccga gctgaaattc ccgtgggaca
     2821 actggatgtt gatgggttgc
//
LOCUS       DASUGB010000935         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1354635, whole genome shotgun sequence.
ACCESSION   DASUGB010000935 DASUGB010000000
VERSION     DASUGB010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354635"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFV87_17770"
     CDS             <1..729
                     /locus_tag="VFV87_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5105675.1"
                     /translation="AERLAAMGQTIATLSHHIKNILQGVRGGSYLIDEGLKEGDNDVV
                     RRGWRIVERNQERISNLVMDMLSFSKDREPELMPSDLNETITDVLELMKTRAAEAEVE
                     IAFQPRPDLPQFLYDPDLLHRAVLNVVTNAIDACEKREGARVEVALEWTPGDRLIHIV
                     VSDNGEGIAPDDIKRIFSVFESRKGARGTGLGLPVSQKILREHGGEIRVESAPGSGSR
                     FYLELPANFPQLSGDTLAAESPST"
     gene            complement(730..1638)
                     /locus_tag="VFV87_17775"
     CDS             complement(730..1638)
                     /locus_tag="VFV87_17775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5105676.1"
                     /translation="MSSAEHDTVEIPLPGVRSTARPQLPSIQVAVELGAKTHPGLVRP
                     INEDSYLVARADRSLETLLTNLKPGEIPAWEAERSYGLVVADGMGGHAAGEVASHVAL
                     RAVVEHVLATADWVMRDVAMHHAVIEERLAQRFAAADEAVQDEASRNPRWAGMGTTMT
                     LAVSSGANLFLGHIGDSRAYLLRGGNMQVLTRDHSYAQALADAGFISQSEVAAHRMRN
                     VLLRSLGGGSVQADVRHLPLHSGDRLLLCTDGLTDMVAEPVIAEILQSAATAQAACDL
                     LIASALAAGGRDNVTVVLARYAWQQV"
     gene            complement(1678..3192)
                     /locus_tag="VFV87_17780"
     CDS             complement(1678..3192)
                     /locus_tag="VFV87_17780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5105677.1"
                     /translation="MRVQPEIMEELNPCSPPQTPTEAPRAERTGWWSRPCGGREVLAI
                     ALPLVVQTAFWSILWFVDRMFLTWHSADEMAAALPAGMVFWTMIGLPQGVASFVNTFV
                     AQYYGAGRHDRIGRAVKQGIWFGWLVTPLFLLAIPLAPGIFRGIGATEQIAGLEALYF
                     QTLALGAGGLVISAAMSSFYTGRGLTRVVMLVDVAGTLVNIFLDWVLIFGKFGFPELG
                     IAGAGIATAIANWSLVLIYWLLMRRSPDRQKFGLAVNIIDWGLLKRLLRYGVPSGLPM
                     LVEALAFTFLMKQITSIGRAEAAATSLALTVNSVAFVPLIGLSIAVSTLVGQKLGENK
                     PDLAARATWTALTLGLAYTAIFAALYLGLPGWFLVAHEAQADPKAFVPIRELTLVLLH
                     FVALYCLFDALQIVFVGALKGAGDTRFILLNTLTVSTIALLTGNWCAGHFQWAKHGWQ
                     LWGWWWVFTGWIFTLGVVYLARFLQGRWRTMRVIEPDVALEDASAEPVLESVCG"
     gene            complement(3265..>3773)
                     /locus_tag="VFV87_17785"
     CDS             complement(3265..>3773)
                     /locus_tag="VFV87_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5105678.1"
                     /translation="LKAATSGRGSDGEQPRPLVAALQREPIELGRSETLRPGQDGTIL
                     CLGTLLSDCLAAADLLAADGLEVGVVNMRFAKPIDTGAIERALRDTDFVLTVEEGCLM
                     GGFGSAVLETASDLGVDTSRLKRLGLPDRFIEHGERSELLSDLGLDPTGIATACRELA
                     ERGEGVNA"
BASE COUNT          703 a         1208 c         1268 g          594 t
ORIGIN      
        1 gccgagcgcc tggccgcgat gggtcagacg atcgccaccc tctcgcacca catcaagaac
       61 atcttgcagg gcgtacgcgg cggcagctac ctgatcgacg aaggactcaa ggaaggcgac
      121 aacgacgtgg tccgccgcgg ctggcggatc gtcgagcgga accaggagcg gatcagcaac
      181 ctggtgatgg acatgctgag cttcagcaag gaccgcgagc cggagctgat gccgtccgac
      241 ctcaatgaaa ccatcaccga cgtgctggag ctgatgaaga cgcgcgccgc cgaagccgag
      301 gtggagatcg ccttccaacc gcggccggac ctgccgcaat tcctgtacga tcccgatctc
      361 ttgcaccggg cggtgttgaa cgtcgtgacc aacgccatcg acgcctgcga gaagcgcgaa
      421 ggggcgcggg tcgaagttgc cctggaatgg acccccggcg atcggctgat tcacatcgtg
      481 gtctcggata atggagaggg gatcgccccg gacgacatca aacgcatttt ttctgttttt
      541 gaatcccgca aaggggcccg cggcacggga ttggggctgc cggtcagcca gaaaatcctc
      601 cgcgagcacg gcggcgaaat ccgcgtcgaa agcgccccgg gttccggcag ccgtttctat
      661 ctggagttgc cggccaattt tccccaactg tccggcgata cgctggccgc ggaatcgccc
      721 tccacgtaac tagacctgct gccaggcata gcgggccagg acgacggtca cgttatccct
      781 accgcccgcg gcgagcgccg aggcgatgag cagatcgcag gcggcctgcg cggtcgctgc
      841 cgattgcagg atttccgcga ttaccggctc ggccaccatg tcggtcaggc cgtcggtgca
      901 gaggaggagc cggtcgccgc tgtgcagcgg cagatggcgg acatcggcct ggactgagcc
      961 gccacccaag ctgcgcagca gaacgttccg catccggtga gcggcgacct ccgactgcga
     1021 aataaagccc gcatcggcca gagcctgggc atagctatga tcgcgggtga gaacctgcat
     1081 gtttccgccc cgcagcaagt acgcccgcga atcgccaatg tgccccagga acaagttcgc
     1141 gccgctgctc accgccagcg tcattgtcgt ccccattcct gcccagcggg ggttgcggct
     1201 ggcctcgtcc tgcacggctt catcggcagc agcgaaacgc tgggccagcc gctcctcaat
     1261 gacggcgtgg tgcatggcga cgtcccgcat cacccagtcg gcggtcgcca gcacgtgctc
     1321 gacgactgcc cgcaaggcga cgtgactggc gacttccccc gcggcgtgac ctcccatgcc
     1381 gtcggccacg accagtccat aggatcgctc ggcttcccag gcgggaattt cgcccggttt
     1441 gagattcgtc agcaaggttt ccagcgagcg atcggcgcgg gccacgaggt agctgtcctc
     1501 gttgatcggc cgcaccaggc cggggtgtgt cttcgcacct aactcaacgg cgacctggat
     1561 cgaaggtagc tgcggacggg ctgtgctccg cacacccggc aacgggattt cgacggtatc
     1621 gtgttcggcg gaagacatgg tcgtgtcgcc gttcgctgcc actgacctgg tcaccactca
     1681 cccacaaacg gattctaaca ccggctcggc ggacgcgtct tccagtgcga catccggctc
     1741 gatcacccgc atcgtccgcc agcgcccttg caggaaacga gccagataga ccacgcccag
     1801 ggtgaagatc cagcccgtaa agacccacca ccagccccag agctgccagc cgtgcttggc
     1861 ccactggaag tggcccgcgc accagtttcc agtgaggagg gcgattgtgg aaacggtcag
     1921 cgtgttgaga aggatgaagc gcgtgtcgcc cgcccctttg agcgcgccga cgaagacgat
     1981 ctgcagcgcg tcgaacagac aatacagggc gacgaagtgg agcagcacga gcgtcagctc
     2041 gcggatcgga acgaacgctt tgggatcggc ctgtgcctcg tgggcgacga ggaaccatcc
     2101 cgggaggccg agatagagcg cagcgaagat tgccgtatag gccaggccga gcgtcagagc
     2161 cgtccaggtg gcgcgggccg ccagatcggg tttgttttct cccaacttct ggccgacgag
     2221 cgtcgagacc gcgatgctca agccgatgag cggcacgaag gcgacggaat tcacggtcag
     2281 cgccagcgac gtggcggcgg cttcggcccg gccgatcgag gtgatctgct tcatcaggaa
     2341 agtgaaggcg agggcctcga cgagcatcgg cagcccgctc ggcacgccat agcgcagcag
     2401 ccgcttgagc agcccccaat caatgatgtt gaccgccagc ccgaacttct gtcggtcggg
     2461 cgagcggcgc atcaggagcc agtaaatcag caccagcgac cagttggcga tggccgtggc
     2521 gattcccgcc ccggcgatgc ccagctccgg aaagccgaat ttgccgaaga tcagaaccca
     2581 gtcgaggaag atgttcacga gcgttccggc gacatcgacg agcatgacga cgcgcgtcag
     2641 accgcggccg gtgtagaagc ttgacatggc ggccgagatg acgagcccgc cggcgccgag
     2701 ggcgagcgtc tggaaataga gggcttccag gccggcaatc tgctccgtcg cgccgatgcc
     2761 gcggaagatc cccggcgcca gaggaattgc cagcagaaag agcggcgtca ccagccagcc
     2821 aaaccagatc ccttgcttca ccgcccggcc aatgcggtcg tggcggccgg cgccgtaata
     2881 ttgggccacg aacgtgttga cgaaggaggc caccccttgc ggcaggccga tcatcgtcca
     2941 gaagaccatg ccggccggca gcgccgccgc catttcgtcg gctgaatgcc aggttaggaa
     3001 catccggtcg acgaaccaga ggatcgacca gaaagcggtc tgcacgacga gcggcaaggc
     3061 gatcgccagc acttcccggc cgccgcaggg gcgcgaccac cagccggtgc gctccgcccg
     3121 cggcgcctct gtgggggtct ggggcggcga gcagggattg agctcttcca tgatttctgg
     3181 ttgcacgcgc attgttccca gcctgtgcgc agagttgccg cagtccgtga cggtcggggc
     3241 tatttcttgc cggccgtccg ggcctcaggc atttacaccc tcaccccgtt cggccagttc
     3301 ccggcaggcg gtggcgatgc cggtggggtc gaggccgagg tcggagagga gttcggagcg
     3361 ttcgccgtgc tcgatgaagc ggtcagggag gccgagacgc ttgaggcgag aggtgtcaac
     3421 gcccaggtcg ctggcggttt cgaggacggc gctgccgaag ccgcccatca ggcagccttc
     3481 ttcgacggtg aggacgaagt cggtgtcgcg gagggctcgc tcgatggcgc cggtgtcgat
     3541 cggcttggcg aagcgcatgt tgacgacgcc gacttccagg ccgtcggctg cgagcagatc
     3601 cgcggcggcg aggcagtcgg acaggagtgt gccgaggcag aggatcgtgc cgtcctggcc
     3661 ggggcggagg gtttcagagc ggccgagttc aatgggctct cgctgcaaag ccgcgaccag
     3721 tggtcgcggt tgttcgccgt cggaaccgcg accactggtc gcggctttga ggg
//
LOCUS       DASUGB010000936         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1354640, whole genome shotgun sequence.
ACCESSION   DASUGB010000936 DASUGB010000000
VERSION     DASUGB010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354640"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..960
                     /locus_tag="VFV87_17790"
     CDS             112..960
                     /locus_tag="VFV87_17790"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5105679.1"
                     /translation="MTSNHEHILQSTPLQLGRHTLTSRLIVGTGKYDTFETMQRSLAL
                     SGADCITVAVRRERLYDGRGQNILDFLDLARYTLLPNTAGCYNAADAVRVARMGREIL
                     LSLDNPGADWIKLECLGDAKTLLPDPIETLNATEQLVAEGFSVLVYTSDDPVIARRLK
                     KAGATAVMPAGSPIGSGQGVLNPNAIRIILEYLKDDDPDYPVIIDAGVGTASDVTIAM
                     ELGADGVLLNTGIAHARDPLGMARAMKAACEAGRLAYLSGRIPKRLYAQASSPEEGVI
                     STRPYR"
     gene            978..1619
                     /locus_tag="VFV87_17795"
     CDS             978..1619
                     /locus_tag="VFV87_17795"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEX5105680.1"
                     /translation="MAIEVSCPSCAGQFRVPDTAAKKKIRCPKCKGAMEVPAAPSRSP
                     PTAVETQPTPERWFLKTEDGEDFGPAARAELDEWKAEGRITVDCQLLREGSDQWQWAE
                     DLYPDLHSTPAAAEPGRGGPARSLATTPSKATARPVAAPIECGPTSSRSRRVAAMLGV
                     FLGPLGTHRFYLGYWVLGLLMLATGGGCGIWSLIDAVLVFLGKVPDADGRRLS"
     gene            2164..>2531
                     /locus_tag="VFV87_17800"
     CDS             2164..>2531
                     /locus_tag="VFV87_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5105681.1"
                     /translation="MFYLLANNGPQQGRRYELKGEKYVLGRHPDCDIHIEVGAVSRNH
                     CQIVRDGSGYALEDLGSRNGTFVNDEQPKVEGRRPLRLGDVLRVCEVSFTFLNDTAPK
                     PNTGMRTEEFDGAGLGAFIA"
BASE COUNT          464 a          759 c          841 g          467 t
ORIGIN      
        1 tccactcctt gaaaaattct tcactgattc atccgtgcca ttctgcggtt tgtcagcgca
       61 gactcaaggc gcggagtcgt cctcttcgtt tacgaggact aaactgcgtt gatgacttcc
      121 aatcacgagc atatcctcca atccacccca ctccagctcg gccggcatac gctgacgagc
      181 cggctgattg taggcacggg gaagtacgac acgttcgaga cgatgcagcg gtcgctggca
      241 ctgtcgggcg ccgactgcat cacggtcgcc gtccgccgcg agcgcctgta cgatggccgc
      301 gggcagaaca tcctcgattt tctcgacctg gcacgctata cgttgctgcc gaacactgcc
      361 gggtgctaca acgcggccga cgcggtgcgc gtggcccgaa tggggcgcga gattctgctg
      421 agcctggaca accccggcgc agattggatc aagctcgaat gcctggggga cgcgaagacg
      481 ctgctgcccg atccgatcga aaccctcaat gccaccgaac aactcgtcgc cgagggtttt
      541 tccgtgctcg tctataccag cgacgatccg gtcatcgctc gccgcttgaa aaaagccggc
      601 gccaccgccg tgatgcccgc cggcagcccg attggctctg gtcagggtgt gctcaacccg
      661 aatgccatcc ggatcattct cgaatatctc aaggacgacg acccggacta tccggtgatc
      721 atcgatgcgg gcgttggcac cgccagcgat gtgacgattg cgatggagct gggcgccgat
      781 ggtgtgctac tcaatacggg catcgcccac gcccgcgatc cgctcggcat ggcccgcgcc
      841 atgaaggccg cctgcgaagc cggccgtctc gcctaccttt ccggtcggat ccccaagcgg
      901 ctatatgccc aggcgagcag ccccgaggag ggggtgatca gcacgcggcc gtacaggtag
      961 ggaattaggg ccgtcccatg gcgattgaag tctcgtgccc cagttgtgcc ggccagtttc
     1021 gcgtgccgga tacggctgcg aagaagaaga tccgctgccc gaagtgcaaa ggggcgatgg
     1081 aggttccagc ggccccatcc cgatcacccc ctacggccgt ggagacgcaa cctacgccag
     1141 agcgctggtt ccttaagacg gaagacggcg aggatttcgg cccggcagca cgcgcggaat
     1201 tggacgagtg gaaggccgag ggccggatca cggtcgattg ccagcttctc cgggagggtt
     1261 cagatcagtg gcagtgggcg gaggatttgt acccggacct gcactccacg ccggccgcgg
     1321 cagagccagg gcggggtgga cctgcaagat cgctggcaac cactccgtcc aaagcaactg
     1381 ccaggccagt cgccgcgcct atcgaatgcg gcccgaccag cagccgctcg cgcagggtcg
     1441 cggccatgtt gggcgttttc ctcggaccgc tgggaacaca ccggttttat ctgggatact
     1501 gggtgctagg ccttctgatg ctcgccacgg gtggcggctg tggcatttgg tcgctgattg
     1561 acgccgtgtt ggtcttcctc ggcaaggtgc cggacgccga tggccggcgg ctgtcttagg
     1621 ctcgccactc cgatcgatcg ggatcggctg ggagttttgg ggaacgcggg tggaggccag
     1681 ggagcaggag tcatagggag gagagagacg aattgacgtg caacagctca ttgtcctcga
     1741 gtgggcgcgc ggtgctgtgg aagcgattgc agggaaaaga gttgtggatc gatggtgcga
     1801 ggaggttgcg cggtaagaaa tttgcggatg tcgcacgagg acgcgcaccg gtgatcgggc
     1861 aagtcgccgc cgattcgtca cgaatctgca gtgctttggg cgcggcggga ccgggctgga
     1921 cttgatttaa gggcggccgg gggttgattc gaaaagtgcg gacggcacgc aagatggctg
     1981 cgccgtagcg ctcatttcac tgagcgcgat ggctatttgc ggaatttgcg gttggttgac
     2041 gatgcggatg gacccccctt acaatcgacc cgatacctct gacggccgga tttcgcgcat
     2101 tcggcccgtt tcctccggcg gtgcgccgtt atccgcgtta tcgcctccgc aggatccgcg
     2161 gctatgttct acttgctcgc caacaacggt ccgcagcaag gccggcggta cgagctgaag
     2221 ggcgagaagt acgtcctcgg ccggcatccc gactgcgaca ttcatatcga agtcggcgcg
     2281 gtcagccgca accactgtca gattgtccgc gacggctcgg gctacgccct cgaggatctc
     2341 ggcagccgca acggcacgtt cgtcaacgac gagcagccca aggtggaggg ccggcggccg
     2401 ctccgattgg gagacgtgct gcgggtttgc gaggtcagct ttaccttcct gaacgacacc
     2461 gcgccgaagc cgaatacggg aatgcggacg gaggaattcg atggggcggg gctgggtgca
     2521 ttcatcgccg a
//
LOCUS       DASUGB010000937         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_799033, whole genome shotgun sequence.
ACCESSION   DASUGB010000937 DASUGB010000000
VERSION     DASUGB010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799033"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2365
                     /locus_tag="VFV87_17805"
     CDS             <1..>2365
                     /locus_tag="VFV87_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105682.1"
                     /translation="QFARVSTPPKPSAFPTLPGLMVGDTIVGISGQPLFGGDRGPARV
                     ESMLTILAQEGKPITLTISRGLPNPYTSHPRLDLFISDNSPHPLQTFACTVCHEGQGS
                     ATDFKWASHTPNDLIEADEWIEEHGWFDNHHWIFPMHPKRFAESTCLKCHHEVVELEA
                     SEKFPVAPAPKVTHGYHLIRKYGCYGCHEINGFDGPAKRVGPDLRSEPNYFAVAQQIQ
                     AELNASQRRLSTARPAGETASPSLSEELERLNVWKTRLQPLAARLASHPDEDATRSEL
                     KQTIEDGVADAGEDALPLDRQLGVLANMLKDVETPGDLRRTGPSLRYLGQKVDRVFLY
                     DWLANPTHFRPTTRMPRFFGLWDHLQNAQGEMTDPHAPELEPIEIRSLIEYFASYSQD
                     QQFELEQRPEGIDDWTDDEKIARGKQQFQVRGCLACHTHKDFPEVAEYRAAGEIVQGP
                     DLSAVGSKFAKQRNPAGPDWLYSWIKNPARYHVRTVMPNLFLEPEMVPGPDPMKPDEG
                     AKRFDPADDVATYLLAASVSDWQPIPAAASATQPLPEGDPALKALMLEYLNEAFYKDA
                     AADYFVNGIPPELEGELKGAEKDLIIQPGEHLTDAQRLKYLGRKTIAKYGCFGCHDIP
                     GFEDAKPIGTGLADWGRKDPSRLAFEHITHYIEHAHGHAASAGHAAESEPPGEIYTAE
                     TQPQIDEQRTVDYFRHALIAGNRIGFIYQKLKEPRSYDYEKTENKRYNERLRMPQFNF
                     TVEEREAIITFVLGLVAEPPREKYLFQPDERQAALMAGQKALEKYNCG"
BASE COUNT          481 a          816 c          727 g          341 t
ORIGIN      
        1 agcagtttgc ccgcgtttcg actccgccaa agccgagcgc ctttcccacg ctccctggcc
       61 tgatggtcgg cgacaccatc gttggcatca gcggccaacc cctgtttggc ggcgaccgcg
      121 gcccggcccg cgtcgaatcg atgctgacga tcctcgccca ggaaggcaag ccgattacgc
      181 tcacaatcag ccgcggcctg cccaatccct atacgtctca tccgcggctg gacctgttca
      241 tcagcgataa cagcccgcac cccttgcaga cgtttgcctg caccgtctgc cacgagggcc
      301 aggggagcgc caccgatttc aaatgggcct cgcacacgcc caacgatctg atcgaggccg
      361 acgaatggat cgaggagcac ggctggttcg acaatcacca ctggatcttc ccgatgcacc
      421 cgaagcgctt cgcggagagc acgtgcctga agtgccacca cgaagtcgtc gagctggaag
      481 ccagcgaaaa gtttccggta gcgcccgcgc ccaaggttac gcacggctac cacctgatcc
      541 gcaagtacgg ctgctacggc tgtcatgaga tcaacggctt cgacggtccg gcgaagcgcg
      601 tcgggcccga tctccgcagc gagccaaact acttcgccgt cgcccagcag attcaggcgg
      661 agctgaacgc ctcgcagcgg cggctttcga ccgcccgccc cgccggcgaa accgcatcgc
      721 cgagcctgag cgaagaactg gaacgcctga acgtctggaa aacccgcctc cagccgctgg
      781 ccgcccgcct ggcttcgcat cccgatgagg acgcgacccg cagcgagcta aagcagacga
      841 tcgaggacgg cgtcgccgac gcgggcgagg acgccctgcc gctcgaccga cagcttggcg
      901 tgctggcgaa catgctcaaa gacgtggaaa cgccgggcga cctgcgccgc accggcccga
      961 gcctccggta tttgggccag aaggtcgatc gggtcttcct gtacgactgg ctcgcgaacc
     1021 ccacgcattt ccgcccgacg acgcgaatgc cccggttctt cggcctgtgg gatcacctgc
     1081 agaacgccca gggcgaaatg accgatccgc acgctcccga gctggagccg atcgagattc
     1141 gcagcctgat cgaatacttc gcctcgtatt cccaggacca gcagttcgag ctggagcagc
     1201 gccccgaggg gatcgacgac tggaccgacg acgaaaaaat cgcccgcggc aagcagcaat
     1261 tccaggtccg cgggtgcctg gcgtgtcata cgcacaagga ttttcccgag gttgcggagt
     1321 accgagcggc tggcgagatc gtgcaggggc cggacctgtc cgccgtgggc agcaaattcg
     1381 ccaaacagcg caatccagcg ggccccgact ggctctatag ctggatcaag aacccggcgc
     1441 ggtatcacgt ccgcacggta atgcccaacc tgttcctcga gccggaaatg gtaccggggc
     1501 ccgacccgat gaagcccgat gagggcgcga agcggttcga cccggccgac gacgtcgcca
     1561 cgtacctgct ggcagcgtcg gtcagcgact ggcagccgat ccccgccgcg gccagcgcca
     1621 cgcagccgct gccggaaggc gatccggcgc tgaaggccct gatgctggag tatctgaacg
     1681 aagcgttcta caaggacgcg gccgccgatt acttcgtcaa cggcatcccg ccggaattgg
     1741 agggcgagct gaagggggcc gagaaggacc tgatcatcca gcccggcgag cacctgaccg
     1801 acgcccagcg gctcaagtac ctgggacgta agacgatcgc caagtacggc tgcttcggct
     1861 gccacgacat ccccggcttc gaggatgcca aaccgatcgg caccggcctg gccgattggg
     1921 gccgcaagga tccctcgcgg ctggcgttcg aacacatcac gcactacatc gagcacgcgc
     1981 acgggcacgc cgccagcgcc ggccatgcgg ccgagtcgga gccgccgggc gagatctaca
     2041 ccgccgagac gcagccgcaa atcgacgagc agcggaccgt cgattacttc cgccacgccc
     2101 tgattgccgg caaccggatt ggcttcatct accagaagct caaggagccg cggagctacg
     2161 actacgaaaa aaccgaaaac aagcgctaca acgagcgcct gcggatgccg cagttcaatt
     2221 tcacggtcga agagcgcgag gccatcatca cgttcgtgct gggcctcgtc gccgagccgc
     2281 cgcgcgagaa atacctgttc cagccggacg agcggcaggc cgcccttatg gccgggcaga
     2341 aagcgctgga gaaatacaac tgcgg
//
LOCUS       DASUGB010000938         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1910212, whole genome shotgun sequence.
ACCESSION   DASUGB010000938 DASUGB010000000
VERSION     DASUGB010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910212"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFV87_17810"
     CDS             complement(<1..734)
                     /locus_tag="VFV87_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105683.1"
                     /translation="MASWSSAQSPSQASSQPSAATEADWILLHEDAVLDELKLSPTQR
                     SELRSALDPLDLAVFQLRNRPPKESSAQFANVVAQAKAAATQVLKADQRARLDKLVAR
                     YLGTRAFQLESVRQRLNLAENQRTAIDAAIAQSKTALAKLRNESQGKTPEELNMQAAA
                     IQKEEQSAVRAALDDAQKRKFATLSASDFDLSRLGRTRFKVPELIGQVADWRNSPPLS
                     LAQQRGRVVVIHFFACGCINCIRNYPT"
     gene            1209..>2279
                     /locus_tag="VFV87_17815"
     CDS             1209..>2279
                     /locus_tag="VFV87_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105684.1"
                     /translation="MYRAVPAQEVARSRRELAAAVRDVEAWLNRSGADRAAGWKRYLH
                     WSELENIVAQEGAPEADSVQTILRKLSASENGLENRRMVRMRQALKAYADVATAAADT
                     RLQETYAQNLDELAKHLEAYQQNPAAGDEALAVAKGLDWLARTGQASDLQSDIREQFG
                     QPNLFASVSERFAAVGVEQNVDQLTGVRDNILGTDIHGTARMTGHTSLAFLDNPRAAT
                     FNIFLAGTAVSNNVGYNGPVTVYTSGVTSISASKQIQMTADGLVGYRSGARCATRTNI
                     RDLCANCGLIEKIAWRRAGEQKAEAEAIASSHAASRVAGQLDGETGGLIAEQNANYQR
                     RFKQPLVRRNAFPERLVFSSTSD"
BASE COUNT          431 a          728 c          726 g          394 t
ORIGIN      
        1 gtcggatagt tgcgaatgca gttgatgcag ccgcaggcga aaaagtgaat gacgaccacc
       61 ctgccacgct gctgggcaag agacagcggg ggggaattgc gccagtccgc aacctggccg
      121 atcagctcgg gcactttgaa ccgggtgcgt cccaggcggc tcagatcgaa atcggaggcc
      181 gagagagtgg caaacttccg tttttgcgcg tcgtcaagcg cggcgcgcac ggcggattgc
      241 tcctccttct gaatcgccgc agcttgcatg ttgagctctt ccggcgtttt cccctggctc
      301 tcattccgaa gcttggccag cgccgttttc gactgggcaa tggcggcgtc gatcgcagtc
      361 cgctgattct cagcgagatt gagccgctgg cggacgcttt ccagctgaaa agccctcgtg
      421 ccgaggtatc gggcgacgag cttgtccaga cgcgcccgct gatcggcttt caacacctgg
      481 gtcgcagccg ccttcgcctg agcgacgaca ttggcaaact gcgcgctgga ctccttcggc
      541 ggccgattgc ggagctgaaa cacggccagg tcgagcggat ctagcgccga tcgcaattcg
      601 ctgcgctgcg tgggcgagag cttcagctcg tccaggacgg cgtcttcgtg cagcaggatc
      661 caatcggctt cagtcgcggc ggatggctga gacgacgcct gagacggcga ttgagcggac
      721 gaccatgatg ccaccccacc aaccactaac aagcataagg ccggcacgac cacacgaggc
      781 aacgacattg ccagcgactc cagaattcat gccctgaggc attgtatccc cgccctgagg
      841 tcatcgccac gagcggcgac gtatgaatcc attgcgtccg gcagcgtggc tcagccggct
      901 tggataccta ggccgacttt gccgccggcg ttgattggat tgattggggt gattgttcgg
      961 cttgcgaaat gcagccattc ggcggaagat tgggcgattc acggatgcgc cgcagccggc
     1021 agggcgaaat cgctgtttcc ggctgtcttt tcccgaagtc aggagctggg ttcatgttgc
     1081 gacattttac gaccttgctt gccggtgccc ttttggcaag tgccgcttgc ttcccggcgc
     1141 gcgggcagga aacatccact gccaaggtgc cggtttcgtt ggctgaggcg gcccgccagg
     1201 ccaaacaaat gtaccgagca gttcctgcgc aggaagtcgc ccggtctcgc cgcgagctag
     1261 ccgcggcggt gcgtgatgtc gaagcctggc tcaaccgcag cggcgccgat cgggccgccg
     1321 gctggaagcg ctacctgcac tggagcgagc tcgagaatat tgtcgcccag gaaggcgcac
     1381 ccgaggccga cagtgtccag accatcctgc gaaagctatc ggccagcgag aacggcctgg
     1441 agaaccgccg catggtgcgg atgcgacagg cgctcaaggc ctatgccgac gtcgccacgg
     1501 cagcggccga tacccggctg caagaaacct atgcgcagaa cctcgacgag ctggccaaac
     1561 acctcgaagc ctatcagcag aacccggcgg cgggagacga ggcgctggca gtggccaagg
     1621 ggctcgattg gctcgcccgc accggtcagg cgagcgacct gcagtcggac attcgcgagc
     1681 agttcggcca gcccaacctg ttcgcctcgg tgtccgagcg cttcgccgcg gtgggcgtgg
     1741 agcagaacgt cgatcagctc accggcgtgc gcgacaacat ccttggcacc gacatccacg
     1801 gcacggcccg catgacgggc cacacgtcgc tcgcctttct cgacaatccg cgagccgcga
     1861 cattcaacat cttcctggct ggcacggccg tatcgaacaa cgtcggctac aacggtcccg
     1921 tcacggtcta tacgtcgggc gtcacgagca tctcggccag caagcaaatc cagatgacgg
     1981 ccgatgggct cgtcggttat cgctccggcg ctcgctgcgc tacgcggacg aatattcgcg
     2041 acctgtgcgc caactgcggc ctgatcgaaa aaatcgcctg gcggcgggca ggcgagcaga
     2101 aagccgaggc cgaggcgatc gcctccagtc atgcagccag tcgcgtggct ggccagctcg
     2161 atggtgagac cggcggcctg attgcagagc agaacgccaa ttaccagcgc cgcttcaaac
     2221 agccgctcgt ccgccgcaac gccttcccgg agcggctggt gttcagctcg accagcgac
//
LOCUS       DASUGB010000939         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_522230, whole genome shotgun sequence.
ACCESSION   DASUGB010000939 DASUGB010000000
VERSION     DASUGB010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522230"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..531
                     /locus_tag="VFV87_17820"
     CDS             145..531
                     /locus_tag="VFV87_17820"
                     /inference="COORDINATES: protein motif:HMM:NF025716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEX5105685.1"
                     /translation="MSRTSLNFILDSLLLTAFSVLVWSAVVVRFVFPPGTSAAGWLLW
                     GLSYDQWINVQFAMVAILALGILIHVMLHWTWVCGFVAARLGRGKKARLDDGTQTLYG
                     VGLLIMIFNLLGLAIASAALSIQSPP"
     gene            1475..2026
                     /locus_tag="VFV87_17825"
     CDS             1475..2026
                     /locus_tag="VFV87_17825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105686.1"
                     /translation="MADAEAWEVFVEIYEPLLFRLARGRGMQSADAEDYVQEVLAAIV
                     RNINRWVASENRGSFRAWLFRIAFNLGINFLTRPKHQRLGAGGSVSARMLAEAPDASA
                     DSSEQFLKEYRRELFRWAAERVREQVSERQWMVFWQTTVEERPISEVAEESAMSVGSI
                     YIARSRITKRIREMIREHEEQSQ"
     gene            2023..>2948
                     /locus_tag="VFV87_17830"
     CDS             2023..>2948
                     /locus_tag="VFV87_17830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105687.1"
                     /translation="MTTGSDSCQDVHLDVLLDGDEDSDLFRRAAGHVESCPECQRRLT
                     ELSGDAEHWSVQREMLRPVSGDRPEAGLLCRYRSAESAPWRRHVIDESTVKELLTAPS
                     HPEMLGRLGRYEIERIIGTGGMGIVLKGFDSELNRPVAIKLLAPHLARVGAARQRFAR
                     EARAAAAVVHEHVVPIHNVESEHATPFLVMQFIPGESLQARVEREGPLSVTEILRIGM
                     QAAAGLAAAHTQGLVHRDVKPANILLENGVERVYLTDFGLARASDDASLTYTGVVAGT
                     PHYMSPEQADGQPLDHRSDLLSLGSVLYFMATG"
BASE COUNT          531 a          879 c          944 g          594 t
ORIGIN      
        1 cgtcggctct ttgccgccgc ggcctggtgc ctgatactga cgggccttgt gtccgtcgcc
       61 cgcggcgtct cgtttttgtc catcgatggc caaccgccag ccggctgccc tatgtgccag
      121 aattagccct gcccacccag ccgcatgtcg cgcacgagcc tcaatttcat tctcgactca
      181 ctgctcctga cggctttttc ggttctggtc tggtcggcgg ttgtcgtgcg atttgtcttc
      241 ccgccgggaa ccagcgcggc aggctggctc ctatggggtt tgagctatga ccagtggatc
      301 aacgtgcagt tcgcaatggt tgcgatcctc gccctgggca tcttgatcca cgtcatgctg
      361 cattggactt gggtctgcgg cttcgtcgcc gcccgcctgg gccgcggcaa gaaagccagg
      421 ctcgacgacg gcacgcaaac gctctacggc gtcggcctct tgatcatgat cttcaacctc
      481 ctcggcctcg ccatcgcctc cgcggcgctt tcaattcaat ctccaccgtg atgccaagtc
      541 catgttgagc cggttcagcc cgattgaacc tcatgatgat gtgcgacatc tgcagaaaag
      601 cgagccgcgg cgctgcggtc acatcgcccg aagggcttaa gaacgcccga aaactcggca
      661 catgatagtg gcgcgggaga atagctcgtc gaatcagcgt agatccggtt gatgccatgg
      721 cgacgtgggc gcccccgacg atggacgcca ccgagatcga gaggctcaag attaattcac
      781 aggggaaccc ggtcgaagtg accgaaacag cgtgctagac acaccatggg cgtcggcgtg
      841 agaatgggcg gagcgaccgc caggactttt agactgggca acatggggac gctcttatgc
      901 aagtcggtcg gcgagttatc gttgcgggca ttctgctggt gccggggctt ttttcctgca
      961 atctctcgcg tgccgacgaa ggcgaggacg atcagaaacg cgcggaagca ttgtcgttgt
     1021 taaagagcat cgcggcggag tatgacatcc gcttggctca cgcccgtacg gccgcgcgcg
     1081 agtcgcagcc cgcaattcgc tggacgaatc cgatcagcgg cgtcaccgac gcggctcttt
     1141 tcatctggac gtacgagtac cgtcccgttg cggtgggaag catggtttgg ctcggggact
     1201 cgggcgtcag tcgtgagttt cagtccttgt cgtccgagcc gctggaggct cgtcgcggag
     1261 gaaacgtttc ctggagtcct ggcgcgaacg ctggagcgtc cgttattagg acgcactgcg
     1321 gttgcaatct ggcttgcaac catcggtaat cgaatgaaag ctttttgcga ttgccgtgtc
     1381 ttactcaata aaggaattct ctctccacca gtcgcactga cctgtgccac aaccaccaga
     1441 aacccgcgag acgctgattc gacggctgcc gaatgtggcg gatgccgagg cgtgggaagt
     1501 gtttgtggag atctacgagc cgctgctgtt tcggctggct cgggggcggg ggatgcagtc
     1561 ggcggacgcc gaggattacg tccaggaggt gctggcggcc atcgtcagaa acatcaatcg
     1621 ctgggtggcc agcgaaaacc gcggctcctt tcgagcgtgg ctctttcgaa ttgccttcaa
     1681 tctcggcatc aatttcctga ctcgccctaa gcaccaacgg ttgggtgccg gaggcagcgt
     1741 gagcgcacgg atgctcgcgg aagcgccaga cgcgtcggcg gactcgtcgg agcagttcct
     1801 gaaggaatac cggcgcgagc tgtttcgctg ggcagccgag cgggtgcgcg agcaggtttc
     1861 cgagcgacaa tggatggtct tctggcaaac cactgtcgag gagcggccaa tttccgaggt
     1921 cgcggaggaa tctgccatga gtgtcggcag tatttatatc gcgcgcagtc ggatcacgaa
     1981 gcgcattcgc gaaatgattc gagagcatga ggagcaatcg caatgaccac tggctccgac
     2041 tcttgccaag acgtccatct cgacgtcctg ctggacggcg atgaggattc ggacctgttt
     2101 cgccgcgcgg caggccacgt cgagtcttgt cccgagtgcc agcggcggct cacggagctg
     2161 tcaggcgacg cggagcactg gtccgtccag cgcgagatgc tgcggcccgt ttcaggcgac
     2221 cggccggaag ccggattgct ctgtcgctat cgctccgccg agtccgcacc atggcgtcgc
     2281 cacgtgatcg atgagtccac ggtgaaggaa ttgctcacgg cgccgtcgca tcctgaaatg
     2341 ctcggtcggc tgggacgcta cgagatcgaa cggatcatcg gcacgggggg catggggatt
     2401 gtgctgaagg ggttcgactc ggaattgaac cggcccgtgg cgatcaagct gctcgcgccg
     2461 cacttggcgc gcgtgggcgc ggcccggcag cggtttgcgc gcgaagcgcg cgcggcggca
     2521 gcggtcgtgc acgagcatgt cgtgccgata cacaatgtcg agtcggagca cgcgactcca
     2581 ttcctggtga tgcagttcat tcccggcgag tcgctgcagg cccgcgtcga acgcgaggga
     2641 cctctcagcg tcacggagat tcttcgcatc ggcatgcaag cggcagcggg tctggcggcc
     2701 gcacacactc aaggactcgt gcatcgcgat gtcaagccag ccaacatcct gctggaaaac
     2761 ggcgtcgagc gggtttacct gaccgatttc ggcctggccc gagcttccga cgatgccagc
     2821 ctcacctaca ccggtgtcgt cgctggaacg ccgcattaca tgtcacccga acaggccgat
     2881 ggccagccgc tcgatcatcg gtcggacttg ttaagtctcg gctcggtgct ctatttcatg
     2941 gctacggg
//
LOCUS       DASUGB010000940         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2050745, whole genome shotgun sequence.
ACCESSION   DASUGB010000940 DASUGB010000000
VERSION     DASUGB010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050745"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..871)
                     /locus_tag="VFV87_17835"
     CDS             complement(134..871)
                     /locus_tag="VFV87_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX5105688.1"
                     /translation="MSPTLVTFLFEAANFLLLAAVLSWLLFKPVRKALADYRARLDAQ
                     AIKAQEKLAASEKLQADIEQERQAFRDESARLRNDTLAAARTQAEQTLAQARERARTV
                     KEAAEQHVTRLDAAQKEALAQAAAMAAAAAVAKLLEQIGGPELETALRQAACRQLAGL
                     QLDGQRIIIESAAPLGDSERVELEHALGPAAVHVTYRVVKELRGGIRVTTSRGVIDAS
                     VAGLADYARIALTSELGEAASTGATHA"
     gene            complement(878..1141)
                     /locus_tag="VFV87_17840"
     CDS             complement(878..1141)
                     /locus_tag="VFV87_17840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5105689.1"
                     /translation="MTDESVIAIVSISAAALTMALGAIGAALGESRVGAAAMDALARQ
                     PDEAGSITRTLFVSLAMVESTAIFCLVIALIVLFANPFITAPK"
     gene            complement(1138..1812)
                     /gene="atpB"
                     /locus_tag="VFV87_17845"
     CDS             complement(1138..1812)
                     /gene="atpB"
                     /locus_tag="VFV87_17845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5105690.1"
                     /translation="MTLHIFGEPVLFYVGSVPVTETMATSVGVSLLIVFATAVLRVAV
                     VRAPQHWLSTLALMTFEWLDSLVRDVLGREHPLVTALVGSLFLFIAGCNLAGQLPGTH
                     PVTASLATTSALAAVVFFAVPIAGIASHGVWGYVRHYFRPNPLFIPLHVISELSRTLA
                     LSVRLFGNVMSGHLVVALLVALGGFLVPTPMMALDVLIGLLQAYIFMVLSTVYIGAAI
                     SVGENA"
     gene            complement(2057..2341)
                     /locus_tag="VFV87_17850"
     CDS             complement(2057..2341)
                     /locus_tag="VFV87_17850"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5105691.1"
                     /translation="MNRGFQRRDVRGALARDASRRTRRERGHGSFWRSLSVLGMVGWP
                     IALAASGGALLGRFLDQRLDTGVQLTLMLLTAGTILGSYIAWYTIQQQKQ"
     gene            complement(2338..2760)
                     /locus_tag="VFV87_17855"
     CDS             complement(2338..2760)
                     /locus_tag="VFV87_17855"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105692.1"
                     /translation="MDNAVLHVVVRTPQRVVVEREVTSLRFPTETGQVGMRPRSESCV
                     LAFEPGLVLLRRGERTEFVGTAGGLLRCDGIIANLMTPLAVVGDRLDDVLTELDRALE
                     TPTAEHEVRTMLSRLEQNILQELQTDGESHSGRARSPS"
     gene            complement(2753..>3254)
                     /locus_tag="VFV87_17860"
     CDS             complement(2753..>3254)
                     /locus_tag="VFV87_17860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5105693.1"
                     /translation="VSVQAVYVPADDYADPAITHTFWHMDSALVLSRDIAAEGIYPAV
                     DPLASSSKALDSAIVGEQHYDVAQEARRVLGRYDELKDLISMLGIDELSAEDRQLVGR
                     ARKLRNFFTQPFFVAEPFTGTPGRSVTPAATVAGVAAILDGQCDQLPEQSLFMIGELK
                     EAMGHG"
BASE COUNT          666 a         1079 c          943 g          566 t
ORIGIN      
        1 gtcgagaccg gaaaccagag ccacgccatc accgacgcgt cgcacgaccc cgcgcggctc
       61 gatccgctgg gccagcgaca caccgctgga gaacagccgc cctgcgagcc gctggacgat
      121 atcttccggc ggtttaggca tgggttgctc ccgtcgaagc tgcttcgccc aactcggaag
      181 tcagagcaat tcttgcgtaa tccgccagtc ccgccacgct ggcgtcgatc actccgcggc
      241 tggtagttac gcgaattccg ccacgtagtt ccttgaccac gcgatatgtg acatggaccg
      301 ccgctgggcc gagcgcatgt tcaagctcca cccgttcact atcgcctagc ggcgcagcag
      361 attcgatgat gattctttgc ccatcgagtt gcagtccagc cagttggcgg caggcagctt
      421 gacgcagtgc ggtttccaat tcgggcccgc cgatctgctc cagcagcttt gccactgcgg
      481 ccgcggcggc cattgcggcg gcctgcgcca aggcctcctt ttgcgcggcg tcgagccgcg
      541 ttacgtgctg ctcggcagcc tctttgacgg tgcgggccct ttcgcgcgct tgagcgaggg
      601 tttgttcggc ttgggtccga gcggcagcca gcgtgtcgtt gcggagtctg gccgactcgt
      661 cgcgaaacgc ctggcgctcc tgctcgatat cggcttgcaa cttttcggat gcggcgagct
      721 tctcctgagc cttgatggcc tgcgcatcga gccgcgcgcg atagtcggcc agggccttgc
      781 ggactggctt gaacaacagc caggacagca cggcagcgag taggaggaaa ttcgccgcct
      841 cgaacaggaa ggtaacgagc gttggcgaca caattgttta cttgggtgcc gtgatgaacg
      901 gattagcgaa aagcacgatc agcgctatca ccaggcaaaa gatcgcggtc gattccacca
      961 tcgccaagct gacaaacagc gtccgcgtga tcgaacccgc ttcatccggc tggcgagcca
     1021 gggcatccat cgcagccgcg ccgacgcggc tctcgcccaa cgctgcgcca atcgcaccta
     1081 gcgccatcgt cagcgcggcg gcagaaatgg aaacgatggc aatcaccgat tcgtcggtca
     1141 tgcattttct cccacgctga ttgcggcgcc aatgtacacc gtcgacagca ccataaaaat
     1201 gtaagcttgt aataagccga tgagaacatc gagggccatc atcggcgttg gcactaggaa
     1261 tccgccaagg gccaccagca gtgcgaccac caaatgcccc gacatgacgt tgccgaacaa
     1321 tcgcaccgac agcgcgagcg tgcgcgatag ctcgctgatc acatgcagcg gaataaagag
     1381 cggattgggg cggaaatagt gccgcacgta gccccaaacg ccgtgactag cgattccggc
     1441 aatcggtacg gcgaagaaca ccaccgccgc cagcgccgac gtcgtcgcca ggctggcggt
     1501 caccggatgc gtccccggta gttgacccgc caaattgcag cccgcgatga acaggaacaa
     1561 acttcccacc agcgccgtca ctagcggatg ttcacgtccc agaacatcgc ggacgagcga
     1621 atcgagccac tcgaacgtca tcagagccag cgtcgacagc cagtgctgtg gcgctcgaac
     1681 cactgccacg cgcaggacgg cggtcgcgaa aacgatcagc aacgaaacgc caaccgaggt
     1741 ggccatggtc tcagtcaccg gcaccgagcc aacgtagaac agcactggct cgccaaaaat
     1801 gtgcagcgtc atgcaggtct gctccttgcg aggaccgctg ccgcggtgac aacaaatccc
     1861 gctccccaac ccatgccagc gggcatcaaa ccgcccgtga tcgcactcgc aacaagaacg
     1921 cctgccatga tgattagtcg caacataccc agtgctgcat gccaggatgc aaactgccgc
     1981 gcggaccgcc agagcgatgc cgcgtgcagc gaccccgcgc acaggcctgt gagcgcccaa
     2041 agtatttgcc agagcgtcat tgcttctgtt gctggatggt gtaccaggcg atgtacgagc
     2101 ctagtatagt cccggcggtg aggagcatca gggtcagctg aacgccggtg tccaaacgtt
     2161 ggtcgagaaa acgtcccaac agcgcgcccc cgcttgcggc cagtgcaatg ggccagccga
     2221 ccatccccag cacgctcagc gatcgccaaa aactgccatg cccccgttcg cggcgtgtcc
     2281 gccgcgaggc atcgcgcgcc agcgcacccc gcacatcgcg ccgctggaag cctcgattca
     2341 tgacggactc ctcgcgcggc cggaatgcga ttcgccatcg gtctgcaatt cctggaggat
     2401 gttctgttca agccgcgaga gcattgtccg cacttcgtgc tcggcggtcg gcgtttctaa
     2461 agctcgatcg agctcggtga gcacgtcgtc caaacgatcg ccgacaaccg caagcggtgt
     2521 catcaagttc gcgattatgc cgtcgcagcg caagagccca cctgcggttc ctacgaactc
     2581 tgtccgttcg cctctgcgca gaaggacgag ccccggctcg aaggccagta cgcacgactc
     2641 gctccgtggt cgcattccaa cctggccggt ctcggtggga aaacgtagcg acgtcacctc
     2701 gcgttccacc accacgcgct ggggtgtgcg gacgacgacg tgcaggaccg cgttatccat
     2761 gccccatcgc ctccttcagt tcgccgatca taaacaggct ttgctcagga agttgatcgc
     2821 actggccatc cagaattgcc gccacgccgg ccaccgtggc cgccggcgtc acgctgcgtc
     2881 ccggcgtgcc ggtgaatggc tcggcgacga aaaacggctg cgtgaaaaag tttcgcaact
     2941 tccgcgcccg tcccaccaat tgacgatctt cggccgataa ttcgtcgatc ccgagcatcg
     3001 agatcaaatc ctttaattca tcgtaccttc ccagcactcg gcgggcctct tgggccacgt
     3061 cgtaatgctg ctcgcccaca atcgccgaat cgagcgcctt cgacgacgag gccaaaggat
     3121 cgaccgccgg atagatccct tcggccgcaa tatcgcgcga caggaccagc gcgctatcca
     3181 tgtgccagaa ggtatgtgtg attgcgggat cggcgtaatc gtcggccggt acatacactg
     3241 cctgcaccga cacc
//
LOCUS       DASUGB010000941         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_799884, whole genome shotgun sequence.
ACCESSION   DASUGB010000941 DASUGB010000000
VERSION     DASUGB010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799884"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2450
                     /locus_tag="VFV87_17865"
     CDS             <1..2450
                     /locus_tag="VFV87_17865"
                     /inference="COORDINATES: protein motif:HMM:NF024906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105694.1"
                     /translation="IGFPGDDAAWKAFGEYQKSEIGPLFFRNKQPVNADGTFTIENML
                     PGSYQFFVDGTHVAYRQFTVEPEVAGQAPGALDLGEIAVKVAAVPKPDNGAAAAPAEP
                     GAKPTKLDEFLQRVRQAQRGPFGGNPVTMSQNGQIVGLGLAEFELRPGDAAIVGQLPE
                     LSRLSLQRSNVTDEDLQQFKALKKLTTLNLWDTKISDAGIAAMSELTSLERLQLGGTD
                     VTDAGLDSVARLKSLQELDLARTKITDAGLKKLAPLWKLQGLKLAETSVSDAGLAALD
                     RLPALRGITLDETSVTATGLERLAMRAPLNYMGSDKLVAQELAERVSKGDVAGVEAML
                     SIGVDLPHAGKFKTRSVTAHPVTARDAEEQRKRFRIEWDWSNNGKDEGLFAEIAVRQG
                     TASVIEAGVLEADVKTTAKAEAKTVAIRGKAVDDETGRPVAPLIVQAGKFDPADPTKV
                     TWGFSEGRSSAMDGSFSTTIRWADGWTARIIADGYLPQPVLAKAPPEGQEEIEVLVRL
                     KRGKLVRGQVLDHKDQPVKGAAVFAVGPTGVNLAGGKAWNSWGGDDNVPKSAITDEKG
                     HFELPAGEAKKVAVSGSVIDAWPAEIAAEGETIVKLPEPAKVEIRLDIEGADKESEVF
                     FQLLTSNMKGFDGVRIERTLPIANGGKLTLPALPPGKYQFCRQVMNRLGEIGTGAMLQ
                     REFIELKAGESRAVNYARPKGARVRGKIGVPAGVELMGIVVSVEAEKPEKSPFDDHEW
                     TTTYASQTAAADGSFLTERIAPGTYVLKVEAYVPLTPEQRVRTGIIGPTYHAKAKIEV
                     PESGELMVPDLKLEAKR"
     gene            complement(2460..3686)
                     /locus_tag="VFV87_17870"
     CDS             complement(2460..3686)
                     /locus_tag="VFV87_17870"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX5105695.1"
                     /translation="MDRRLFLATTMSTGMIAATNLPETQLAAAEKYTVRGLTKSNIPT
                     PALVVDLEALEANLRKMAEHARQAKVGLRPHAKTHKCPEIARRQIAAGAIGICCATVP
                     EMEAMAAAGIPGLLVTSPIVDPGKIGRVAALASGGNKVMLSVGHVREAELLAEAAEAA
                     KAELDVLVDLDVGDKRTGCLPGEPAVELARQMARFQRLHIRGVQAYAGHASHTVGFEA
                     RQRVSREAMSKAVETKALLLKAGFDAQILSGGSTGTYNIDSAIEGVTELQVGSYVFMD
                     VDYRRIGGQDGNATYADFQPSLTILATVVSATHAGRVTIDAGTKAVDTTTANLPQPKN
                     WPGLVYSKAGDEFGALTPSEPGGKLPRLGERLEFIVPHCDPTVNLYDRLYACRGENVE
                     EIWPVVARREVPGKPA"
BASE COUNT          747 a         1198 c         1293 g          654 t
ORIGIN      
        1 acattggctt tccaggcgat gacgccgcct ggaaggcctt cggcgagtac cagaagagcg
       61 aaatcggccc gctcttcttc cggaacaagc agccggtcaa cgccgacggc acgttcacga
      121 tcgagaacat gctgccgggc tcgtaccaat tcttcgtcga tggcacgcat gtcgcctacc
      181 ggcagtttac cgtcgagccg gaagttgccg gccaggcgcc aggcgcactc gacctcggtg
      241 aaattgcggt caaggttgcc gccgtgccca aaccggacaa cggcgccgcg gcagcgccgg
      301 cggaaccggg cgcgaagcca acgaagctcg atgaattcct gcagcgcgtg cggcaagcgc
      361 agcgcgggcc gtttggcggc aatccggtca cgatgagcca gaacggccag atcgtcgggc
      421 tggggctggc tgaatttgaa ttgaggccgg gcgacgcggc gattgttggt cagctgcctg
      481 agctgtcgcg gctcagcctg cagcgttcca acgtgacgga cgaggatttg cagcagttca
      541 aggcgctgaa gaagctgacc accctcaatc tctgggacac caagatcagc gatgcgggca
      601 tcgcggcaat gagtgagctg acatcgctcg agcgcctgca attgggtgga acagacgtga
      661 cggacgccgg gctggacagc gttgccaggc tcaagtcgct gcaagagctg gatctggcgc
      721 gaacgaaaat cacggacgca ggcctgaaga aactcgctcc actctggaag ctgcagggat
      781 tgaagctggc ggaaacctcg gtaagcgatg cgggccttgc cgcgctcgac cgattgcccg
      841 ctctccgcgg catcacgctc gacgaaacga gcgttactgc gacgggtctg gaacgcctgg
      901 cgatgcgggc gcccctgaat tacatgggat ccgacaagct ggttgcgcag gagctcgccg
      961 agcgagtgtc gaaaggggac gtggcgggcg tggaggcgat gctctcaatc ggcgttgacc
     1021 tgccgcacgc aggcaagttc aagacccgct cggtcacggc ccatcctgtc acggccaggg
     1081 acgccgagga gcagcggaag cgattccgca tcgaatggga ttggtccaac aacgggaaag
     1141 acgaaggctt gttcgccgaa atcgccgtgc ggcagggaac cgcatccgtg atcgaggcgg
     1201 gagttctgga ggcagacgtg aaaaccaccg cgaaagccga ggccaagacc gtcgccatcc
     1261 gcggcaaggc ggtcgatgac gaaacgggca ggcccgtcgc cccgctgatc gtacaggcag
     1321 gcaagttcga cccggctgat ccgacgaagg taacgtgggg ctttagcgag ggccgctcca
     1381 gcgcaatgga tggctcgttc tcgacgacca ttcgttgggc cgacggctgg acggcgcgga
     1441 tcatcgccga cggctacctg cctcagccgg tattggccaa agcgccgccg gagggccagg
     1501 aagagatcga ggtgctcgtg cgcctgaagc gtgggaagct ggtgcgtggg caggtgctcg
     1561 atcacaagga ccagccggtg aaaggcgcgg cggtgttcgc cgtcggccca accggcgtga
     1621 acctggctgg gggcaaggct tggaacagct ggggcggcga cgacaacgtg cccaagtctg
     1681 cgattacgga tgagaagggt catttcgagc tgccagccgg agaggcgaag aaggtggcgg
     1741 tgtctggcag cgtcatcgac gcctggccgg ccgaaattgc cgcggaaggc gaaaccatcg
     1801 tcaagctgcc cgaaccggca aaagtcgaga ttcggctcga tatcgaaggg gccgacaagg
     1861 agagcgaggt tttctttcaa ctgctcacgt ccaacatgaa aggtttcgac ggcgtgcgga
     1921 tcgagcgcac gctgcccatc gccaacggcg gcaagctgac gctccccgcc ctgccgcccg
     1981 gcaagtacca gttctgccga caggtaatga accggctggg tgagattggc accggcgcga
     2041 tgctccagcg ggaattcatt gagctgaaag cgggcgagtc gcgggcggtc aactacgccc
     2101 gtcccaaagg cgcgcgggta cgtggcaaga tcggcgtccc ggcgggagtg gaactcatgg
     2161 gcatcgtcgt ctcggtcgaa gccgaaaagc cggagaaaag cccgtttgac gaccacgagt
     2221 ggaccacaac ctacgcttcg cagaccgccg ctgccgacgg cagcttcctg accgagcgga
     2281 tcgccccggg aacctacgtg ctcaaagtgg aagcctacgt gccgctcacg cccgagcagc
     2341 gcgtgcggac cggcatcatc gggccaacgt accacgcaaa ggcaaagatt gaagtccccg
     2401 aatcgggcga gctgatggtg ccagacctga agctggaggc gaaacggtag agcgtcgccc
     2461 tatgccggtt tgcccggaac ctcgcggcgg gcgactacag gccaaatttc ttcgacgttt
     2521 tcgccccggc aggcgtagag gcggtcgtag aggttcacgg tggggtcgca gtgggggacg
     2581 atgaattcca ggcgctcgcc gagcctgggg agcttgccgc cgggttcgct cggcgtcagc
     2641 gcgccgaatt cgtcgccggc tttggagtag acgagtccgg gccagttttt tggctgcggg
     2701 aggttggcgg tggtggtgtc gacggctttc gtgccggcgt cgatcgtgac gcgaccggcg
     2761 tgcgtggcgc tgacgaccgt ggcgaggatc gtcaggctgg gctggaagtc ggcataggtc
     2821 gcgttgccgt cctggccacc gatccggcgg taatcgacgt ccatgaacac atacgagccg
     2881 acctgcaatt cggtgacgcc ttcgatcgcg gagtcgatgt tgtacgtgcc cgtgctgccg
     2941 ccgctcaaaa tctgggcgtc gaagcctgcc ttgagcagca gagccttcgt ttcgacggct
     3001 ttggacatgg cctcgcggct gacgcgctgg cgggcctcga agccgacggt gtgcgaggca
     3061 tggccggcat aggcctgcac gccgcggatg tgcagacgct ggaaacgggc catctgccgg
     3121 gccaattcca ccgccggctc accgggcagg caaccggtgc gcttatcgcc gacatccaga
     3181 tcgacgagca cgtccagttc tgccttcgcc gcctccgccg cttcagccag cagttccgcc
     3241 tcgcggacgt ggccgacgga gagcatcact ttgtttcctc cgctagcgag tgctgccaca
     3301 cggccaatct tgcccggatc gacgatcggc gacgtgacca acagtcccgg aataccagcc
     3361 gcggccatcg cttccatctc cggcaccgtc gcgcagcaga tgccaatcgc ccccgccgct
     3421 atctgccggc gggcgatctc cgggcacttg tgcgtcttcg catgcggccg caagccgact
     3481 tttgcctgcc tggcatgctc cgccatcttt cgcagattgg cctccaacgc ctctaaatca
     3541 accaccagcg ccggcgtcgg aatattggac ttcgtcagcc cgcggacggt gtatttctcc
     3601 gccgccgcga gctgcgtttc aggcaggttg gtcgctgcga tcatgccagt gctcatcgtc
     3661 gttgccaaaa acaagcggcg gtccataggt gcaatctccg ctatccgtcg cagcatcaac
     3721 ccttctgcaa tgctgtccgc tattccttca gaagcgtttc gaccacgcgg tcaagctcgg
     3781 agttggcaag gtccgccaca cgaaggttgc cgctccgatc gatgacgtag tagtcgggat
     3841 agctatcgac gtggaaagcc gacaccgtct tcttctccac atccacggca ac
//
LOCUS       DASUGB010000942         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_799966, whole genome shotgun sequence.
ACCESSION   DASUGB010000942 DASUGB010000000
VERSION     DASUGB010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799966"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFV87_17875"
     CDS             <1..1177
                     /locus_tag="VFV87_17875"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEX5105696.1"
                     /translation="ISTPICQRLQKEGHEQIIRERTGLLIDPYFSGTKIAHLLESISG
                     LRQRAERGEILFGTVDSFLIWRLTGGQTHATDVSNASRTLLFNIHTLQWDDELLRILN
                     IPRAMLPEVCPSSHVFGKCDRDLLGEPIPIAGVAGDQQAATFGQACFEPGSAKNTYGT
                     GCFLLLNIGETPRLSENKLVTTVGWQVDGKTTYCLEGSVFIAGAVVQWLRDGLGIIKT
                     SSEVQQLATSVPDSDGVVVVPAFVGLGAPYWDPHARGAIFGISRGTTAAHIARAAVES
                     MAFQSRDLLEAMRADAGLPLSVLKVDGGASVNDGLMQFQADILDAAVRRPVVAETTAL
                     GAAYLAGLAVGYWSDLADVAKNWALDREFIPAMPYAERQSRCQLWQEAVRRTLGWVK"
     gene            complement(1195..1584)
                     /gene="rbsD"
                     /locus_tag="VFV87_17880"
     CDS             complement(1195..1584)
                     /gene="rbsD"
                     /locus_tag="VFV87_17880"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEX5105697.1"
                     /translation="MLQTGILNPHVQSLLARVRHTNTLVIADRGFPFWPAIETIDLSL
                     VDNVPTVLQVLASIRANFVVGHAWMAHEFQQHNSPDVQSTFAAALQGISLTCEPHSEF
                     KRRVPQAIGLIRTGDTIQYANIILESG"
     gene            1714..2886
                     /locus_tag="VFV87_17885"
     CDS             1714..2886
                     /locus_tag="VFV87_17885"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5105698.1"
                     /translation="MSIERLTRRIAVGFLAVVTSHIVAAEPNLRRADLENQLAPHYHP
                     PAEFAGQFGDYKSPLTFADGSAIKTKQEWARRRAEILVLWRERLGPWPPLVEKSKLET
                     LDTVERDGYVEHHVHVQVSNEGQVADGYLLIPPGKGPFPAVLVPFYEPLTSIGRGQKG
                     RGTHDYGLQLVKRGFVTLSIGTPGSIENIGKETRELLTEAGKQRRIQPLTLLAYVSAN
                     CHTALSRMPEVDPERIGIIGLSYGGKWSMFSSCLYDKFACAVWSDPGIVFNEKNSNVN
                     YWEPWYLGYDPNEQRQPGVPSNSNPRTGLYKDLVASGDDLVDLHALMAPRPVLVSGGT
                     EDPPRNWVALNHLVAVNDLLGVKNRVAMTARKSHVPTADALELELAFLEYWLGKNE"
     gene            3032..>3685
                     /locus_tag="VFV87_17890"
     CDS             3032..>3685
                     /locus_tag="VFV87_17890"
                     /inference="COORDINATES: protein motif:HMM:NF015916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HEX5105699.1"
                     /translation="MAAIMSAQEPATEKPQFKEGESVEVWWAGSYLPGEIVGLEENGW
                     VRVQFAVNGREQTIKQPGDGQWVRKRKAPPTAPAAPAAGRPRPAAEPANPFATAAETK
                     RRTWTDSTGKFKIEASFSGKEGNDVLLTRADGKTIKLPLAKLSKEDQEHVAKISSQPP
                     PAENPFAAAPEVADNTVDRTADWSTVEQVLPQPVDGWPLAPDVAAPPAEPLAGRAIGL
                     "
BASE COUNT          713 a         1131 c         1179 g          662 t
ORIGIN      
        1 catcagcacg ccgatctgcc agcggctgca gaaagagggc cacgagcaga tcatccgcga
       61 gcgcacggga ttgctcatcg atccctactt ctctggcacg aagatcgccc acttgctgga
      121 gtcgatctcc ggcctccgcc agcgggccga gcgcggcgaa atcctgttcg gcacggtcga
      181 ttcgttcctg atctggcggc tgaccggcgg ccaaacgcac gcgaccgatg tcagcaacgc
      241 cagccgcacg ctactgttca acattcacac gctccagtgg gacgacgagc tgctgcggat
      301 cctgaatatc ccgcgggcca tgctgccgga ggtctgcccg tcgagccacg tcttcggcaa
      361 gtgtgaccgc gacctcctgg gcgagccgat cccgatcgcg ggcgtcgccg gcgaccagca
      421 agccgccact tttgggcaag cttgctttga accggggtcg gcgaagaaca cctacggcac
      481 cggctgcttc ctgttgctga acatcggcga gacgccgcgg ctgagcgaga acaagctggt
      541 gacgaccgtt ggctggcagg tggacggcaa gacgacgtac tgcctggaag gctccgtctt
      601 catcgcgggg gccgtcgtgc aatggctgcg cgatgggctc ggaatcatca aaacctcgtc
      661 cgaggtccag cagctcgcga cgagcgtgcc cgattcggac ggggtagtcg tcgtgccggc
      721 gttcgtcggt ctcggcgccc cctactggga cccgcacgcc cgcggggcga tcttcggcat
      781 tagccgcggt acgacggcag cccacatcgc ccgggccgcg gtcgaatcga tggcctttca
      841 gtcgcgcgat ctcttggaag ccatgcgggc cgacgctggg ctgccgctct cggtgctcaa
      901 ggtcgatggc ggcgcctcgg tcaacgacgg cctgatgcaa ttccaggccg acattctcga
      961 cgccgccgtc cgccggcccg tcgtcgccga aaccacggcg ctcggtgcgg cctatcttgc
     1021 cggcctcgcc gttggctatt ggagcgacct ggccgacgtc gccaagaact gggccctgga
     1081 ccgcgagttc atccccgcca tgccctacgc cgagcgccag tcccgctgcc aactctggca
     1141 agaagccgtc cggcgcacgc tcggctgggt gaaatagcca acgcgctgtg caggtcaccc
     1201 ggattccaga atgatatttg cgtattgaat tgtgtccccg gttcggatca agccgatcgc
     1261 ctgcggcaca cggcgcttga attcggagtg gggctcgcag gtgagcgaga taccttgcag
     1321 agctgcggca aaggtggatt gcacatctgg cgaattgtgc tgctggaact cgtgagccat
     1381 ccaagcgtgg ccgaccacaa aatttgcacg gatcgacgcg agcacctgca gaacagtcgg
     1441 aacattatca accaacgaca aatcgatagt ctcgattgcc ggccaaaatg gaaatccgcg
     1501 atcggcaatc accagcgtgt tcgtatgcct cacgcgggca aggagcgatt gcacgtgtgg
     1561 attgaggatg ccagtttgaa gcatgacgtg gtttgactcg ctattttgcg attgtcgcgg
     1621 acttctgttc caattccttc aaaggattga gcagaagaac gcatcggacg ctaagatgcc
     1681 ggagcgtcaa tttcaagcat ggggaggtgt gtgatgtcga tcgaacggct gacgcgaagg
     1741 atcgccgtgg ggtttctggc ggtcgtcaca agccatattg ttgctgccga gcccaacctg
     1801 cggcgcgccg atctcgaaaa tcagctcgct ccgcactatc atccgccagc ggagttcgca
     1861 ggccaattcg gcgactacaa gtcgccactg acgttcgccg acggctcagc gatcaagacg
     1921 aagcaggagt gggcgcggcg gcgggcggag atactggtgc tctggcgcga gcggctcggg
     1981 ccttggccgc cgctcgtcga gaaatcgaag ttggagacgc tcgacaccgt cgagcgcgac
     2041 ggctacgttg agcaccacgt acacgtgcaa gtgtcgaacg aaggccaggt ggccgacggc
     2101 tacctgttga ttccgcccgg gaaaggtcca tttcctgccg tgctcgtgcc attctacgag
     2161 ccgctgacga gcatcggccg cggccagaaa gggcgcggca cgcacgacta cggtctgcag
     2221 ctcgtcaagc gcggtttcgt aacgctctca atcggcactc cgggctccat cgaaaacatt
     2281 ggcaaggaaa cgcgggaact cctcaccgag gcgggcaagc agcgacgcat tcagccgctg
     2341 acgctcctgg cctatgtctc tgccaactgc catactgctc tatctaggat gccggaggtt
     2401 gatcccgagc ggatcggaat catcgggctc tcctatggcg gcaagtggtc gatgttttcg
     2461 tcatgtctgt acgacaaatt cgcttgcgcg gtctggtctg atccaggcat cgtcttcaac
     2521 gagaagaaca gcaacgtcaa ttattgggag ccgtggtacc tgggttacga cccgaatgaa
     2581 cagcgccagc ccggagtgcc ttccaattcc aatccgcgga cggggctgta caaggatctg
     2641 gtcgccagtg gcgacgacct cgtcgatctg cacgccctga tggctccgcg gcccgtcctc
     2701 gtcagtggcg gcaccgaaga tccgccccgc aattgggtcg cgctcaacca tctcgtcgcg
     2761 gtcaacgacc tgcttggcgt caagaatcgc gttgcgatga cggctcgcaa atcgcacgtg
     2821 ccaacggccg atgcacttga attggaactg gcattcttgg agtactggct gggcaagaat
     2881 gagtgaaccg acgaatcagt tgccaacggc tgcgatggca atttatattc gagccactgc
     2941 cttcttcgat cgcatattca gcggagaccg cctgatgcac aattccccat cactgagcct
     3001 tcgttgtgga accttcctcc tggtgatatt gatggcggcc attatgtcgg cccaggagcc
     3061 agcgacggag aagccacagt tcaaagaagg agagtcggtc gaggtgtggt gggccgggag
     3121 ctatctgccg ggtgagatcg ttgggctgga agagaacggc tgggtgcggg tgcagttcgc
     3181 tgtcaacgga cgcgagcaga cgatcaagca gccgggcgat gggcagtggg tacgcaaacg
     3241 gaaggcgccg ccgacggctc cggcggctcc ggcagcgggt cgtccacggc ctgcggcaga
     3301 gccggcgaat cccttcgcga ccgcagccga gacgaagcgc cgcacgtgga ccgacagcac
     3361 gggcaagttc aagatcgagg cgagcttctc cggcaaagag gggaacgacg tgctactgac
     3421 gcgggccgac ggcaagacga tcaagctgcc actggccaag ctgagcaagg aagatcagga
     3481 gcacgtcgcg aagatctcga gccagccgcc gcccgctgag aatccatttg ctgctgctcc
     3541 ggaagtggcc gataacacgg tcgatcgcac tgctgactgg tcgacggtcg agcaggtcct
     3601 gccgcagccg gtcgatggct ggccgctggc gcccgatgtg gctgccccgc ccgcggagcc
     3661 gctggcgggg cgggcgatcg gcctg
//
LOCUS       DASUGB010000943         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_383770, whole genome shotgun sequence.
ACCESSION   DASUGB010000943 DASUGB010000000
VERSION     DASUGB010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383770"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFV87_17895"
     CDS             <1..1182
                     /locus_tag="VFV87_17895"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HEX5105700.1"
                     /translation="PPPNIAAIEPDVRGATTIREQLAKHREHAVCASCHSRIDPPGFA
                     LEAFDVIGGFRTRYRSIGEGDNAPRGSIDPFIGISFKLGQPVDASGQLSDGTAFENVR
                     EYQALLAQDTERLLWNLARQLVVYATGRENRFGDRPALDDIVSRTQARGGGLRTLLHE
                     VVQSPLFTGRNSDRSRTDQPPAFAAASADERDNSRMMLTGPLPEVGSTANYVATVHPA
                     PEPPPAKPTTIDGNSTITLRVIGLFEPARGDDLEAALKQIPEVKLVQLDRDLAEVTLA
                     YDPNCDLLKNATPEQLIERIGNRLRDLTHHTLKLAARSDIPRDKLQRVEIPILGLDCQ
                     ACCLAAYEAIAQVEGVEQATASFRESQAIAWIHPDKTDRAALEQALLRRGVTLDQPKS
                     E"
     gene            1315..2418
                     /locus_tag="VFV87_17900"
     CDS             1315..2418
                     /locus_tag="VFV87_17900"
                     /EC_number="4.2.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901605 - alpha-amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cis-3-hydroxy-L-proline dehydratase"
                     /protein_id="HEX5105701.1"
                     /translation="MKIARIFAHRVELPLIEGSYKWSGGKSVSVFDSTIVGVETECGL
                     VGYGEVCPLGPFYLPAYAEGVRAGLRELAPHVLGFDPRELAKLNQRMDAALKGHPYVK
                     SGIDIACWDILGKATELPVCTLLGGRFGESVRLYRAISQQSPEAMAKNVQGYRDQGYT
                     RFQLKVGGDPDTDIERIRAVRQLLLPTDRLVADANTGWTQHEALRVVRAVRDVDVYIE
                     QPCLTYEECLAVRRHTDHPFVLDENIDSLDMLLRGKADLAMDVVNLKISKLGGLAKTR
                     QARDLCVSMGIAMTLEDSWGGDITTAAIAHLAHSTPEEFRFTSTDFNSYVTLSTAEGA
                     PRRENGFMSASKAPGLGISPRMEVLGQRALDIS"
     gene            2478..>3393
                     /locus_tag="VFV87_17905"
     CDS             2478..>3393
                     /locus_tag="VFV87_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5105702.1"
                     /translation="MDATIFQGGIPALMTPCRPDRTPHHPALVRKGQELIAAGMNAVV
                     YCGSMGDWPLLSDEQRQEGVRQLTEAGVPVIVGTGAQSPAQAAAHAAHAQQVGATGLM
                     VIPRVLSRGTSPAAQRAHFAGVLGAAPKLPAVIYNSPYYGFETKADLFFDLRREFPNL
                     VGFKEFGGAKSLSYAAEHITHADSDVRLLVGVDTQVCHGFLRCGARGAITGIGNCLPR
                     EVLHLLALCQQAAGGDATARRLAHELEDALGELSRFDEGPDLVLYYKHLLVLLGEKDY
                     ELNLNPTDRLSPSQQQFAAAQLRLFQKWW"
BASE COUNT          613 a         1151 c         1078 g          551 t
ORIGIN      
        1 ccaccgccga acatcgcggc cattgagccc gacgttcgcg gcgcgacgac gatccgcgag
       61 cagctggcca aacatcgcga gcacgccgtc tgcgcctcgt gccattcgcg gatcgatccg
      121 ccgggcttcg cgctggaagc ctttgacgtg atcggcggct tccgcacgcg ataccgctcg
      181 attggcgagg gggataatgc cccgcgcggc tcgattgacc cgttcattgg catcagcttc
      241 aagctcgggc agccggtcga tgccagcggc cagctcagcg acggcacggc gttcgagaat
      301 gtgcgggaat accaggcgct tttggcccag gataccgagc ggctgttgtg gaatctggcc
      361 cggcagctgg tggtttatgc gacgggccgc gaaaaccgct ttggagatcg cccggcgctg
      421 gacgacatcg tcagccgcac gcaggcccgc ggcggcggcc tgcggacgct cttgcacgag
      481 gtcgttcaga gccccctctt caccggccgc aattccgatc ggtctcgcac cgaccagccg
      541 ccggcgtttg ccgcggcctc ggccgacgag cgagacaatt cgcggatgat gctgaccggc
      601 ccgctgcccg aagtcggctc cacggcgaac tacgtcgcca cggtccatcc cgcccccgag
      661 ccgccgccgg ccaaacccac gacaatcgat ggcaatagca caatcacgct gcgcgtgatc
      721 ggactgtttg agcccgcccg cggcgacgac ctcgaagccg ccctcaagca aatcccggaa
      781 gtgaagctgg tgcagctcga ccgcgacctg gccgaagtga cgctggctta cgatccgaat
      841 tgcgatctgc tgaagaacgc gacccccgag cagctcatcg agcggatcgg caaccggctg
      901 cgagacctga cgcatcacac gctgaagctg gccgccagga gcgacatccc gcgcgacaag
      961 ctccagcgcg tcgaaatccc cattctcggc ctcgattgcc aggcttgctg cctcgcggct
     1021 tacgaggcca ttgctcaggt ggaaggcgtc gaacaggcca ccgccagctt ccgcgagagc
     1081 caagcgatcg cctggatcca tccggacaaa accgaccgcg cggcgctcga acaggcgctg
     1141 ctgcgccgcg gcgtcacgct ggaccagccc aagtcagaat agacaagcga cgcctggcct
     1201 gcatcgcccg cgcggcttga cacggcgatg ccctcgcacg acactcggca gcatcgcgtc
     1261 gcctcagcgg atcaagattc tgcgccttga ggatgcaacg gcagtcgcaa ttccatgaaa
     1321 atcgcccgta tcttcgccca tcgagtcgag ctgccgctga tcgaaggctc gtacaagtgg
     1381 tcgggcggca aatcggtttc ggtgttcgac agcacgattg tcggggtcga aaccgagtgc
     1441 gggctggttg gctatggcga ggtctgtccg ctcgggccgt tctatttgcc cgcgtatgcc
     1501 gaaggggtcc gcgccggcct gcgggagctc gcgccgcacg tcctggggtt cgatccgcgc
     1561 gagctggcca aactcaatca gcgcatggat gccgccctca aggggcatcc gtacgtgaaa
     1621 tccggcatcg acatcgcctg ctgggacatc ctcggcaaag cgaccgaact gccggtttgc
     1681 acgctcctgg ggggccgctt cggcgagagc gtaaggctgt accgggccat ctcgcagcaa
     1741 agccccgagg cgatggcgaa aaacgtccaa ggctaccgcg accagggcta cacgcggttt
     1801 cagctcaagg tgggcggcga cccggatacc gacatcgagc gcattcgtgc cgtccggcaa
     1861 ctgctgctgc cgaccgatcg gctggtcgcc gacgcgaaca ccggctggac gcagcacgag
     1921 gccctgcgcg tcgtgcgggc cgtccgcgac gtggacgtct atatcgaaca gccgtgcctg
     1981 acctatgaag agtgtctggc cgtccgccgg cataccgacc atccgttcgt cctcgacgag
     2041 aacatcgact cgctcgacat gctgctgcgg ggcaaggcgg atctggcgat ggacgtcgtg
     2101 aacctgaaga tcagcaagct gggcgggctc gccaaaaccc ggcaggcccg cgacctgtgc
     2161 gtgtcgatgg ggatcgcgat gacgctggaa gacagttggg gcggcgacat caccacggcg
     2221 gcgatcgccc atctcgcgca cagcacgccc gaggaatttc gcttcaccag caccgatttc
     2281 aacagttacg tcaccctcag cacggccgaa ggcgccccgc ggcgggagaa cggcttcatg
     2341 tccgccagca aggccccggg gctgggcatc tcgccgcgga tggaagtcct cggccagcgc
     2401 gccctcgata tttcgtgacg tccgcgacga atggctacga ccacaacgct tcattggcca
     2461 cagtgaaagg ccctcccttg gacgcgacga tctttcaagg cggcattccg gcactgatga
     2521 ccccctgccg ccccgaccgc actccccatc atccggctct cgtaaggaag gggcaggaat
     2581 tgatcgcggc gggaatgaat gccgtggttt attgcggttc gatgggcgat tggccgctgc
     2641 tatcggacga gcagcgtcag gaaggagtac gccagctcac agaggcaggc gtgccggtca
     2701 ttgtgggcac gggtgctcag agtcccgccc aagccgccgc tcacgccgct cacgcacagc
     2761 aggtcggcgc cacgggcctg atggtcattc cccgcgtcct ttcgcgcggc acgtcgccgg
     2821 cggcccagcg ggcacacttt gccggagtgc tcggggcggc ccccaagctg ccggccgtca
     2881 tctacaacag tccctattac ggctttgaga ctaaggcgga cctgttcttc gacctgcggc
     2941 gcgaattccc gaacctggtc ggcttcaagg aattcggcgg agcgaagtcg ctgagctacg
     3001 ccgcagagca catcacgcac gctgattccg acgtgcggct cctggtgggc gtggatacgc
     3061 aagtgtgcca cggcttcctt cgctgcggcg cccgcggggc gatcacgggc atcggcaact
     3121 gcctgccgcg agaggtgctg catttgctgg ccctctgcca acaggccgct ggcggcgatg
     3181 ccacggcccg gcggctggcc catgaactgg aggacgccct gggggagcta tcccgcttcg
     3241 acgaaggccc cgacctggtg ctctactaca agcacctgct cgtcttgctg ggcgaaaaag
     3301 actacgagct aaacctgaac cctaccgacc gcctgtcgcc aagtcagcag cagtttgccg
     3361 ccgctcaact ccggctcttc cagaagtggt gga
//
LOCUS       DASUGB010000944         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1356400, whole genome shotgun sequence.
ACCESSION   DASUGB010000944 DASUGB010000000
VERSION     DASUGB010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356400"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VFV87_17910"
     CDS             complement(<1..1708)
                     /locus_tag="VFV87_17910"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105703.1"
                     /translation="MFSLQGSAARLCDGVSRRELLRVGGLSMLGLSLSRVLAARETAA
                     LGVLPTDKTFGKAKSIIFLWLAGGPPQHETFDPKPAAPLEVRGPFKPISTNVPGIQFC
                     ELLPRTAALADKLAVIRSLSTNDNTHDSSGYWLWTGYKYTGSNSRTIQPNDWPYFGSL
                     VKRLKPSDTLPPLSTVWLPDMARLNENVTPAGQTGGFLGQEWDVDRFVGDPSAPNYQV
                     EGLRLSELPPLRISQRQNLLKQVEKHFAQVERGSAVKLFDRYQQQAFDLMTSGAAREA
                     FAIDKEPDKLRERYGKNRWGQCVLLARRLVEAGVRLVHVQWPREPGDNAVDNPLWDTH
                     AQNAERVEDVLCPMFDVGYTALIEDLDQRGLLDETLVVAVGEFGRTPKINGNGGRDHW
                     GSVFCATFAGAGIAGGQVYGSSDKEGGYPATDKVEPPDFTATIFHQLGIPHTSTFADR
                     EGREHRLTLGQPLYKLLGIEPATAERCQCTGDASRVPPFDEKEMLFDPGFASPATLMP
                     AESPARPRGWKAGPLAGSDGLAPPGASPLIVRKLPGEVLLGIATNGAAGLKIDKGELA
                     ILA"
     gene            complement(1811..1984)
                     /locus_tag="VFV87_17915"
     CDS             complement(1811..1984)
                     /locus_tag="VFV87_17915"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX5105704.1"
                     /translation="MAKYGKKAQKEVKKAMHEKKEGTLRSGKSGRKVKSRKQAIAIGL
                     SKARKKGDKVPKK"
     gene            2077..>2301
                     /locus_tag="VFV87_17920"
     CDS             2077..>2301
                     /locus_tag="VFV87_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5105705.1"
                     /translation="MSLAAYRHKRRFDKSPEPQGKPASSRRSVPSKRKQGGSGKRQFV
                     VQKHAARSLHYDFRLELDGVLKSWAVPKGPC"
BASE COUNT          396 a          766 c          708 g          431 t
ORIGIN      
        1 gggcaaggat ggccagctct cccttgtcga ttttcagtcc cgcggcccca ttggttgcaa
       61 tgccgagcag cacctcgccc ggcagtttgc gaacgatcag cggcgacgca cctggaggag
      121 cgagaccatc cgatcccgcc agcggccccg ccttccagcc gcgaggccgc gcggggcttt
      181 cggctggcat cagcgtggcg ggcgaggcaa agcccggatc gaacagcatc tctttctcgt
      241 caaacggcgg cacgcggcta gcgtcgcccg tgcattggca gcgttccgcc gtggccggct
      301 caatgccgag aagcttgtag agcggctgac ccagcgtaag gcggtgctcg cggccttcgc
      361 ggtcggcgaa ggtgctggtg tgcgggatgc cgagctgatg gaaaatcgtg gcggtgaaat
      421 caggcggctc gactttgtcg gtcgccgggt agccgccttc cttgtcgctg gagccataaa
      481 cctgcccgcc ggcgatcccc gcgccagcga aagtcgcaca aaagacggat ccccagtgat
      541 cgcggccccc attgccattg atcttcggcg tgcggccaaa ttcgccgacc gcgacgacga
      601 gcgtttcatc gaggagcccg cgctggtcca ggtcttcgat cagcgccgtg tagccgacat
      661 cgaacatcgg gcagagcacg tcctcgaccc gctccgcgtt ctgggcgtgc gtatcccaga
      721 gagggttatc gaccgcgttg tcgcccggct cgcgcggcca ttggacgtga acgagccgca
      781 cgcccgcctc gaccagccgg cgggcgagca gcacgcactg cccccagcgg ttcttgccgt
      841 atcgctcgcg gagcttgtcg ggctccttgt cgatggcaaa cgcctcgcgg gccgcgccgc
      901 tggtcatcag gtcgaacgcc tgctgctggt agcggtcgaa gagcttgacc gcgctgcccc
      961 gctcgacctg ggcgaaatgc ttctcgactt gcttgagcag gttctgccgc tggctgatcc
     1021 gcagcggagg cagttcgctc agccgcagac cttcgacctg gtaattcggc gccgaaggat
     1081 cgccgacgaa ccgatcgacg tcccattcct ggccgaggaa cccgcccgtc tgccccgccg
     1141 gcgtgacgtt ctcgttcagc cgcgccatgt cgggcagcca gacggtcgaa aggggcggca
     1201 gcgtgtcgct cggcttcagc cgcttgacga gcgagccgaa gtagggccag tcgttcggct
     1261 ggatcgtccg ggaattcgac ccggtgtatt tgtagccggt ccacaaccaa tagcccgagc
     1321 tgtcgtgcgt gttgtcgttg gtcgagagcg agcggatgac ggccagcttg tcggccagcg
     1381 ctgcggtccg cggcaacagc tcgcagaatt gaatgcccgg cacattcgtc gagatcggct
     1441 taaacggccc tcgcacctcc agcggtgcag ccggcttcgg atcgaacgtc tcatgctgcg
     1501 gcgggccgcc ggccagccac agaaagatga tgctcttggc cttgccgaac gtcttgtcgg
     1561 ttggcaatac gcccaaggcg gccgtttctc ttgctgccaa tactctcgac agcgaaagcc
     1621 ccagcatcga cagcccgccg acccgcagca attcccgccg cgaaactccg tcgcacagcc
     1681 gagctgccga tccctgaagt gaaaacatgc catgctcctg cgtggaaccg gcggcacctg
     1741 ccagctcagc cgcaatccgg ccgattgtac cacgccctgc gggcacgaat ccacatgcgt
     1801 tgatggttca ctacttcttc ggcaccttgt cgcctttctt acgagccttg gacagaccga
     1861 tcgcaatcgc ctgcttacgg ctcttaacct ttcttcccga cttgccgctg cggagagtac
     1921 cctccttctt ctcgtgcatc gccttcttga cttccttctg tgccttcttg ccgtactttg
     1981 ccatggaaat tctcctttgc ccagagtcga ggagcaaagc atgtaccagc ggagcgcgag
     2041 ttgcacacag cgacccagta ccagaggttt ttcccgatgt ccttggcagc ttatcggcac
     2101 aaacgccgat tcgacaaatc gcccgaaccg caaggcaagc ccgcttcatc gcggcgcagt
     2161 gtgccgagca agcggaaaca aggcggttcg ggcaaacgtc aattcgtcgt ccagaagcac
     2221 gcggcccgga gtctgcacta tgactttcgg ctggagctgg acggcgtgct gaagagttgg
     2281 gccgtgccga aggggccatg c
//
LOCUS       DASUGB010000945         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_800724, whole genome shotgun sequence.
ACCESSION   DASUGB010000945 DASUGB010000000
VERSION     DASUGB010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800724"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="VFV87_17925"
     CDS             complement(<1..2154)
                     /locus_tag="VFV87_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105706.1"
                     /translation="MSSTVTDGGNIVIDVRNLTRTFGDLKAVDNVSFQVRKSAIFGLL
                     GPNGSGKSTIIRMLLGILPPTAGEAYVFGHDAFHDAERVKPRVGYMSQRFTLYADLTV
                     RENVQFYARIYGLDRERLQRRYRDVLELTSLTDHAGQLAGTLSGGWQRRLALACSLVH
                     EPDLLFLDEPTAGIDPVARRYLWDLLFELSGRGVTLFVTTHYMDEAERCSDVGYIFLS
                     KLLVLGKPDELKRLPNVTPPGTRRYELRVPAANERLAELRKLPEVRDATLFGETIHAL
                     VDETLAPPKMLQHLGLSADAAEAREIPPSLEDVFVTLTAAAEAQSEQSAPAAAADVPQ
                     EKPPPAPPAERPAPRPAKPGNAVSGLWAILVKEFFHIQRQPITLFFMLVVPVMQTIIF
                     GYAIDTQIENIPTVVLDLDGRQSAREMVAAFENTRRFRIIEYEHDDDSFRRALTSGRA
                     KVGLRIPPEYSDRLLRGEQVQIQVLIDGSDSQVATTAQSTAQLLGLNLSIQKARTKVE
                     ALQAAPARDPMGHPALPIESRTRLLYNPDLQSSHFFVPGLVGIILQLVTLFLTSFAIV
                     RERELGTLEQLFVTPVSRMGLLLGKLLPYALAGFVELLTVLTVMIYVFGVPIHGSVWL
                     LLALSMLFMVCSLSLGLLVSTIARTQLEAVQLAFVIMLPSVLLSGFVFPRSEMPLPIY
                     ILTFGIPATYFIEILRGIVLRSADLWDLVQPVVGLV"
     gene            complement(2161..3288)
                     /locus_tag="VFV87_17930"
     CDS             complement(2161..3288)
                     /locus_tag="VFV87_17930"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5105707.1"
                     /translation="MTTRIIAIAVVAVCLVGLIAYSQFRPQPNFVSGFIEADEIRLGS
                     RVGGRVQAVYAEEGDRALPGQVLIQLEPFDLLERENEARQTLAARTAEYERLTAGLRP
                     EEVAQAKARLDQLQARLDLLEAGPRKQEIEAARGRVRVAEAEVLLAKRIFERRAELFQ
                     KSAIAREEYDAASEDLSSAQATLDVRKNELELLEIGTREEEIREARAKVEEARQAWQL
                     AEGGYRREEVEQAKASRDAAQAALEAIREQKKELVVTSPIHGVVESLDLRKGDLVPAG
                     APVLSVLDNSHMWVRAYVPENRVGLQTGQHIDVTVDSLGSQRFPGEITFIAREAEFTP
                     SNVQTPEDRSKLVFRVKVTLTQHLDKLRPGMSADLWLDSAR"
     gene            complement(3394..>4078)
                     /locus_tag="VFV87_17935"
     CDS             complement(3394..>4078)
                     /locus_tag="VFV87_17935"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5105708.1"
                     /translation="GLTPEATGARNEGPWRHVLGHDGDPLTAQLAFDPGTKCGYSTFG
                     LFHASLVCENVTGKPYDQFAIEALFQPIGVERWTFEYFEGGGDESESKRYGRHPSHSL
                     GMPARDLARIAYCMLREGRWGQKQVIPKWFVDETAAPTHDVKEPELRFQRAAQSFSHG
                     WELPARLTDGRGAGIPQDARFKPGSGGQLMAFVPSLDLVITRQTGSSGSWEYEEYLRR
                     ACAAVVQEE"
BASE COUNT          717 a         1314 c         1329 g          718 t
ORIGIN      
        1 gaccaggccg acgaccggct gaacgaggtc ccacaggtcg gcactgcgga gcacgatgcc
       61 gcgcaggatc tcgatgaagt aggtcgcggg aatgccaaag gtcaggatgt agatcggcag
      121 cggcatctcg ctgcgcggaa acacgaatcc cgagagcagc acggaaggca gcatgatcac
      181 gaatgcaagc tgcacggctt ccagttgcgt ccgggcgatc gtcgagacga gcagcccgag
      241 gctcagcgag cacaccatga acagcatcga cagcgccaag agaagccaga cgctgccatg
      301 aatcggcacg ccgaagacat aaatcatcac cgtcaggacg gtcagcagtt ccacgaagcc
      361 cgccagcgcg tagggaagca gcttgccgag caagagaccc atgcggctga ccggcgtgac
      421 aaagagctgc tcgagcgtgc ccagctcgcg ctcgcggacg atggcgaacg acgtgagaaa
      481 cagcgtcacc agttgcagga tgatgccgac caggcccggt acgaagaagt gggaactttg
      541 caaatccggg ttgtaaagca gccgcgtgcg cgactcgatg ggcaaggcgg gatgtcccat
      601 cggatcgcgg gcgggcgcgg cctgcagggc ttcgactttg gtgcgggcct tttggatgga
      661 tagattgagc ccgagcaatt gcgcggtgct ctgggcggtc gtggcgacct gcgaatcgct
      721 gccgtcgatg agcacctgga tctggacttg ctcgccgcgg agcaggcgat cggagtactc
      781 gggcggaatc cgcaggccga cctttgcccg gcccgacgtc agcgcgcggc ggaacgagtc
      841 gtcgtcgtgc tcgtattcga tgatgcggaa ccggcgcgtg ttttcgaacg ccgccaccat
      901 ctcgcgcgcc gactgccggc cgtccaggtc gagcacgacg gtggggatgt tctcgatttg
      961 cgtatcgatg gcgtagccaa aaatgatcgt ctgcatcacc ggcacgacca gcatgaaaaa
     1021 cagcgtgatg ggctgccgct gaatgtggaa gaattccttg acaagaatcg cccagagtcc
     1081 gctgacggcg ttcccgggtt tcgccggccg tggagctgga cgctcggcgg gcggcgcggg
     1141 cgggggcttt tcctgcggta cgtctgcagc cgccgccggc gcggattgct cggactgcgc
     1201 ttcggccgca gcggtgaggg ttacgaagac atcttccagc gatggcggga tttcccgggc
     1261 ctcggccgcg tccgcggaaa gtcccaggtg ctgaagcatt ttcggaggcg cgagcgtttc
     1321 atccaccagc gcatggatcg tttcgccaaa cagcgtcgcg tcgcgcactt cgggaagctt
     1381 ccgcagctcc gccagccgct cgttcgcggc gggaactcgc agctcgtagc ggcgcgtccc
     1441 cggcggcgta acgttcggca gccgcttcag ctcgtcgggc tttcccagca cgagcagctt
     1501 ggacaggaag atatagccca cgtcgctgca gcgttccgcc tcgtccatat agtgcgtagt
     1561 gacgaacagc gtgacgccgc ggccggagag ctcaaacagc aggtcccaca ggtaacgccg
     1621 ggcgaccggg tcgatgccgg ccgtcggctc gtcgaggaag agcaggtccg gctcatggac
     1681 cagggagcag gccagggcca atcgccgctg ccagcccccg gagagcgtgc cggcgagctg
     1741 gcccgcgtgg tccgtcagcg aggtcagctc cagcacgtcg cgataccgcc gctgcaatcg
     1801 ctcgcggtca agcccataga tgcgggcgta gaactggacg ttttcgcgaa cggtcaggtc
     1861 cgcatacagg gtgaatcgct gcgacatgta tcccacgcgc ggtttgacgc gctccgcgtc
     1921 gtggaaggcg tcgtgtccaa atacgtacgc ttcgcccgcg gtgggaggca gaatgccgag
     1981 cagcatgcga ataatcgtcg atttgccgct gccgttgggc cccagcagcc cgaaaatggc
     2041 agactttcgc acctggaaac tgacgttatc gaccgccttc aaatcgccaa acgtgcgcgt
     2101 caaattccgg acgtcgatga caatgttccc gccgtcggtg acagtgctgc tcatggcggt
     2161 tcaccgggcg gaatcgagcc acaaatcggc cgacatgccg ggccgcagct tgtccaagtg
     2221 ctgggtcagc gtgaccttta cgcgaaatac cagtttcgac cggtcttcgg gcgtctggac
     2281 gttgctgggg gtgaactccg cttcacgggc gataaaggtg atctcccccg gaaaacgttg
     2341 gcttcccagg ctatcgacgg tgacatcgat gtgctgcccc gtctgcaagc cgacgcgatt
     2401 ttccggcacg taggcccgca cccacatgtg gctgttgtcc agaaccgaga gcaccggggc
     2461 gcccgccgga accaggtccc ccttccgcaa gtcgagcgac tcgaccaccc cgtggatcgg
     2521 gctggtcaca accagctcct tcttttgctc gcgaatcgcc tccagagcgg cctgggcggc
     2581 atcgcgggac gccttggctt gctcgacctc ttcccgccga tagccgccct cagcgagctg
     2641 ccatgcctgc ctggcttctt ccaccttggc gcgtgcttcg cggatttctt cctcccgcgt
     2701 gccgatttcg agcagttcca gttcgttctt ccgcacgtcc agggttgctt gcgccgagga
     2761 caggtcttcg ctggcggcat cgtactcttc gcgggcgatc gcacttttct gaaacagctc
     2821 ggctcgtcgc tcaaagatcc gcttggccaa cagcacctcc gcctccgcga cccgcacccg
     2881 cccgcgggcc gcttcgatct cctgcttgcg cggcccggct tccagcaggt cgagacgggc
     2941 ctgaagctga tcgaggcggg ccttggcctg ggcgacctct tccggccgca ggccggccgt
     3001 gagtcgctcg tattccgccg tccgggcggc cagagtttgc cgtgcctcgt tttccctttc
     3061 gagcaggtcg aacggttcca gttgtatcag gacctggccc ggcagggcgc ggtccccttc
     3121 ctccgcatac accgcctgca cgcggccccc tacgcgcgaa cccaagcgga tttcgtccgc
     3181 ttcgatgaac ccggagacga aatttggctg cgggcgaaac tggctgtacg caatcaatcc
     3241 cacgaggcag acggcaacga ccgcaatggc aatgatgcgt gtggtcattc ttcccccgaa
     3301 ccgatctccg tcggcacgca ggataaggga tgtgagacgg caagttggcg gcgatccgag
     3361 cgtgtcattg tagccagaca gagcgaatcc ggctcactcc tcttggacca cggcggcgca
     3421 cgcgcggcgc aggtactctt cgtattccca gcttccgctg ctgccggtct ggcgggtgat
     3481 gaccaggtcc agactaggaa cgaacgccat gagctggccg ccggagcccg gtttgaagcg
     3541 ggcgtcttgc gggattcccg ccccgcggcc atcggtcagc cttgccggca gctcccagcc
     3601 gtgcgagaac gactgggcag cgcgctggaa ccgcagctcg ggctctttga cgtcgtgcgt
     3661 gggggcggcg gtttcgtcca cgaaccattt cggaatgacc tgtttctggc cccagcggcc
     3721 ctcgcggagc atgcaatacg caatgcgcgc caaatcgcgg gccggcatcc ccaggctgtg
     3781 cgacgggtgg cggccgtagc gcttcgactc gctttcgtcc ccgccgcctt caaaatactc
     3841 gaacgtccaa cgctcgacgc cgatcggctg gaagagcgct tcgatggcga actggtcgta
     3901 cggcttgccg gtgacgttct cgcagacgag cgacgcgtga aagaggccga acgtggaata
     3961 gccgcatttg gttccggggt cgaaggcgag ctgcgccgtg agcgggtcgc cgtcgtgccc
     4021 cagcacgtgc cgccaggggc cttcgttgcg agccccggtc gcctcgggcg tcaggccg
//
LOCUS       DASUGB010000946         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_105294, whole genome shotgun sequence.
ACCESSION   DASUGB010000946 DASUGB010000000
VERSION     DASUGB010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105294"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFV87_17940"
     CDS             <1..826
                     /locus_tag="VFV87_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5105709.1"
                     /translation="CDQETAFAFETAGGKPEVVHLNRLLESPRLAEGFQILAIPGGFS
                     YGDDLSAGRIFGNQMRHHLRGCLEEFKAAGKLIIGICNGFQILIKSGVLLPDRADEPI
                     ATLTLNEGGRFEDRWIHLQVASDKCVFLRGIERMYLPIAHAEGKFVARDEATLERLQA
                     AGQLVLRYCMAEGTGDRGQETEDRGQKTEHHSPLSIHHSPLTTIRNPQLLPFPHNPNG
                     AQVNVAGLCDETGRVFGLMPHPERHIDPTQHPRWTREQRERGDGLAVFENAVRFFA"
     gene            complement(943..1749)
                     /locus_tag="VFV87_17945"
     CDS             complement(943..1749)
                     /locus_tag="VFV87_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5105710.1"
                     /translation="MAQWQQQIVLVTGGSSGLGFAIARAFFQAGAEVILSSRDESRLR
                     SAAAALSMPERACTWLTADLTQQSQAESLIEQAHRKHGRLDVLVNCAGKSTRGEVAST
                     TAERFAEFLEINFLSAVRCTQAALPHLLQQRGHVVNIGSLAAKTVGPYLGAYPASKFP
                     LAAYSQQLRLELGPQGLHVLLVCPGPIRRTDAGQRYTQHEGVVPAEALQPGGGVKMKG
                     IDPDWLARRIVRACERREAELIVPSYVRALLTIAAISPSLADWLVRRWTQ"
     gene            1902..>3994
                     /locus_tag="VFV87_17950"
     CDS             1902..>3994
                     /locus_tag="VFV87_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX5105711.1"
                     /translation="MANVRIPPAHQPAPSRRRRNKRLNLERLEPRLLLAADTSASAGI
                     LTHGGICSCPICSGVGLEQFQPEPAQVVSGGGTTSAASIAGLPQLSSRPGAAATIFLD
                     FDGNTEALWGSYTNVVTRVFDRDGDETSFSASEISAIQEIWARVAEDFAPFNINVTTV
                     EPPSFANKVAIRVAIGGNYSDWFCPAAGGVAYVGAFYNSSSNVAYVFEDALGNGNPKY
                     VAEAASHEAGHTFGLYHQAVWNGNQLVEEYNSGDSNWAPIMGVGYYSTRTTWNSGPTS
                     NSPSQIQDDLAVLANSSNGFGYVADDYGGTTATATDLVTSGGNVNTTGLIGNNSDWDL
                     FKFTTDGGALNLSLNVAQFGANLDSALDLLNAAGQTILSSSPSASLGSSLATTLAAGT
                     YYVAVHSSGGYGNLGTYTLSGSVPGGGGGSQTQNPEIEVTVGSVSIADGATVNYGTRT
                     VGTAITRMFVVRNAGTGTLNLTPLNANSIPAGFTLVANLGATSLAPGESTSFKIQMTS
                     TAAGTYSGVISLGNNDSDESPFEITIRGVVEAPEPQAVVRYIDNGASGFSTSGTWYRV
                     TGTGRESDIHRALPGSGTTVATWTFSGLDAGQYRVSATWPGASYYASNAPFSIYNGSQ
                     ALKTVSINQKAASSGFSDAGSQWADLGTVTITGNTLVVRLTNAANNWVVADAIRIESV
                     GSSGTTGGGGGQVEPA"
BASE COUNT          760 a         1274 c         1267 g          693 t
ORIGIN      
        1 ttgcgaccag gaaacggcct ttgcgtttga gacggccgga ggaaagccgg aggtggtgca
       61 tctgaaccgg ctcttggaga gtccgaggct ggccgagggc tttcagattc tggcgattcc
      121 ggggggcttc agctacggcg acgacctgtc ggccggccgc atcttcggca accagatgcg
      181 gcatcacctt cgcggctgcc tggaggaatt caaggcggcg gggaagctga tcatcgggat
      241 ctgcaatggc tttcagatcc tgatcaagtc gggcgtcctg ctgccagacc gggccgacga
      301 gccgatcgcg accctgacgc tgaacgaagg gggcaggttc gaggatcggt ggattcatct
      361 ccaggtggcc agcgacaagt gcgtcttcct ccgcggcatc gagcggatgt atctgccgat
      421 cgcccatgcc gaggggaagt tcgtcgcacg cgacgaggcg acgctcgagc ggctgcaagc
      481 ggcaggacag ttggtgctgc ggtattgcat ggcggagggg acaggggaca ggggacagga
      541 gacagaggac aggggacaga agacagagca ccactcacca ctcagcattc accactcacc
      601 actcaccaca atccgcaatc cgcaattgct tccctttccc cacaacccca acggggcaca
      661 ggtcaacgtg gccgggctgt gcgacgagac gggccgcgtc ttcggcctga tgccgcatcc
      721 agagcggcat atcgatccca cgcagcatcc gcgctggacg cgcgaacagc gcgagcgcgg
      781 cgacggcctg gcggtgtttg aaaatgccgt gcgtttcttc gcgtagggtg ggacaaggcc
      841 gccgcggccg caggcccacc aaggaggctg ggatttgaga tttcgtcctt ggtgcttcaa
      901 caccgtggtg ggcctgcgtt cgctgcgcga gctggtccca ccctactgcg tccagcgcct
      961 gacgagccag tccgccagcg agggcgagat cgcggcgatc gtgagcagcg cgcggacgta
     1021 cgacggcacg atcagctccg cttcgcgccg ctcgcaggcc cggacgattc gccgggccag
     1081 ccaatcgggg tcgattcctt tcatcttcac gccgccgccg ggttgcagcg cttcggcagg
     1141 aacgacgccc tcgtgctgag tatagcgctg gccggcgtcg gtccggcgga tcggccccgg
     1201 gcagacgagc aagacgtgca agccctgggg tccgagctcc aggcgaagct gctgcgaata
     1261 ggcggccagc gggaacttgc ttgccggata ggcgccgagg tacggcccca cggtcttggc
     1321 cgccagcgag ccgatgttga cgacgtgccc ccgctgctgg agcaagtgcg gcagggcggc
     1381 ctgcgtgcag cggacggccg acaaaaaatt aatttccaga aactcggcga atcgctcagc
     1441 cgtcgttgat gcgacttcgc cgcgcgtgct cttgccggcg cagttcacga gcacgtccag
     1501 acgcccatgt ttacggtggg cttgctcgat cagagactcg gcctggctct gctgggtgag
     1561 gtcggcagtc aaccaggtgc aggcccgctc gggcatcgaa agcgccgccg cagccgatcg
     1621 caggcgcgac tcgtcgcgag acgagagaat gacctcggct ccggcctgga aaaaggcccg
     1681 cgcaatggcg aaacccaggc cgctggaacc gcccgtaact agcacaattt gctgttgcca
     1741 ctgtgccatt acaactatgc tggtcgtatc gccgctggcg tcacaattgg tcttttcggt
     1801 tgacagaaac ggttgataac ctaggtttgc tttgctcatc tcgcaagcgg cgctgcagtc
     1861 ccccgtcaga ttctgccgtt gccgcaccca ggagtgccat gatggccaat gtgcgtatcc
     1921 cgcccgccca ccagcctgcg ccgtcccggc gacgccgcaa caagcgtctg aatctcgaac
     1981 ggctggagcc caggctgctt ttggcggcag ataccagcgc cagtgcagga atcctcacgc
     2041 acggcgggat ttgcagttgt ccgatttgct ccggcgttgg gctggaacag ttccagcccg
     2101 agccggcgca ggtcgtctcg ggcggcggta cgacgtcggc cgcctcgatc gccggtctcc
     2161 cgcagctctc cagccggccc ggggcggcag ccaccatttt ccttgatttc gacggcaaca
     2221 ccgaagcgct gtggggaagc tacacgaacg tcgtcacgcg ggtctttgac cgcgacggcg
     2281 atgagacttc atttagcgcg tcggaaatct ccgcgattca ggagatctgg gcccgcgtgg
     2341 ccgaggactt cgccccgttc aacatcaacg tcacgacggt cgagccaccc tcgtttgcca
     2401 ataaggtggc aatccgcgtg gcgatcggcg gcaattactc ggactggttc tgcccggctg
     2461 cgggcggtgt cgcgtacgtt ggggcgttct acaacagctc ctccaacgtc gcctacgtgt
     2521 tcgaagacgc cctgggcaac ggcaatccga aatacgttgc cgaagccgcc agccacgaag
     2581 ccggccacac attcggcctg tatcaccagg cggtctggaa tggcaaccag ctcgtcgaag
     2641 aatacaactc gggagattcg aactgggccc cgatcatggg cgtgggctac tactcgacgc
     2701 gtaccacctg gaacagcgga ccgacgagca attcgcccag ccagattcag gacgatctgg
     2761 cggtcctcgc gaattcctcc aacggctttg gttatgtcgc cgacgactac ggcggcacca
     2821 cggcgaccgc cacggacctg gtgacgagcg gcggcaacgt gaacaccacg ggcctgatcg
     2881 gcaacaacag cgattgggac ctgtttaagt ttacgactga cggcggcgcg ctcaatctat
     2941 cgctgaacgt ggcccaattt ggagccaacc tcgatagcgc gctcgacctg ttgaatgcgg
     3001 caggccagac gatcctgtcg tcaagcccgt ccgcgtcgct cggctcttcg ctggcgacga
     3061 cgctggccgc cggaacctac tatgtcgccg tccattccag cggcgggtat ggaaacctgg
     3121 ggacatacac gctgagtgga tcagttcccg gcggtggggg aggatctcaa acccagaatc
     3181 cggaaatcga agtcacggtg ggcagcgttt cgattgcgga tggcgccacc gtgaactacg
     3241 gaacgcgaac tgtaggcaca gcgatcacac ggatgtttgt cgtccgcaat gccggcacgg
     3301 gaaccctcaa tctcactccg ctcaatgcca acagcatccc ggccggattc acactcgttg
     3361 ccaacctggg cgccacttcg ctggctcccg gtgaatcaac ttctttcaaa atccagatga
     3421 cctccacggc tgcgggcacc tacagtggcg tcatcagtct tggcaacaac gacagcgacg
     3481 aatcgccctt cgagattacc atccgcggcg tggttgaggc ccccgagcct caagccgtcg
     3541 tccgctatat cgacaacggt gcgagcggct tttcaacctc gggaacctgg tatcgcgtga
     3601 ccggaacggg gcgggagagc gacattcacc gcgcacttcc cggcagcggg acgacggtcg
     3661 ccacctggac cttctccgga ctggatgcgg gacaataccg cgtctcggct acctggcccg
     3721 gcgccagcta ctacgccagc aatgctccct tctcaatcta taacggctcg caagctctca
     3781 aaaccgtcag catcaatcaa aaggcagcca gcagcggctt ctccgatgcc ggctcgcagt
     3841 gggcagatct ggggacggtc acgatcaccg gcaatacgct cgtcgtgcgg ctgacgaacg
     3901 ccgcaaacaa ctgggtggtg gccgatgcca ttcgcatcga gtcggtcggt tccagcggca
     3961 ccacgggggg gggcggcgga caggtggagc cagc
//
LOCUS       DASUGB010000947         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1911756, whole genome shotgun sequence.
ACCESSION   DASUGB010000947 DASUGB010000000
VERSION     DASUGB010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911756"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFV87_17955"
     CDS             complement(<1..818)
                     /locus_tag="VFV87_17955"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEX5105712.1"
                     /translation="MFATQACTRGEKGAGRRNRPARRGNIIVLSAFLMVIMMCFVALA
                     VDVGYIYVMQAQLQRAVDAAALAGAGELINGIPEAEAAAKEYLIRNPVGSDVGTIDEA
                     VFANAVVEFDTDHSEDYETKIGNWNPATQAFEETQVMPSGIKVSMTYPNLPFFFARVI
                     GYDDFTLYAQSTAQYQPRDIVLVLDFSASMNDDSTFDAIGKLSQSTVEQSLQNCWNDL
                     GVSYGNLPVVPNWATAQGVPENTSQLIPHVTVQYRYSSVYVTSTKVLTQVKAEFS"
     gene            complement(1027..2334)
                     /locus_tag="VFV87_17960"
     CDS             complement(1027..2334)
                     /locus_tag="VFV87_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX5105713.1"
                     /translation="MPWFTESDTAISRQRVEALVERALAEAKRRICASPRRVLLLPPD
                     ITRMHSGSGWITECFYRLLADSAEVHVIPTLGQHEPHTREQNAQMFGHIPHERIHPHD
                     WRGGCTYLGDVPAEVVKEATGGAADWKFPVWLNSMLLREPWDLVINIGHVVPHEVLGF
                     ANHNKNYFIGLGGKELICAAHMAAACCGIENNLGNLLTPVRQCFNWAEERFLSGLPDF
                     YAQVVLARDAAGQLVHTGVFVGDDLETYLAAARQAREQNITALDEPLKKVVCVMQGDE
                     FFSTWVANKAVYRTRMALADGGELVIIAPGLKRFGEQPDVDALIRKYGYCGTPRVMEL
                     YRQNADLQDLAHATAHLIHGSSEGRFRITYAPGMLSKSDIEQVHFQYADLAETLRRYP
                     IDKLQEGFNEVAGERIYFIPTPSAGLWATKENLYSRASGFGAG"
     gene            complement(2457..>4011)
                     /locus_tag="VFV87_17965"
     CDS             complement(2457..>4011)
                     /locus_tag="VFV87_17965"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX5105714.1"
                     /translation="RAEDVRQLQSLAVQQGSATWGLWGPNPDKYSSWTSHSNRLIPLY
                     AFGMDLSTVRGANSLYRDLDRIRALYGYEPEGTLNPSAEYFDQTDVYRLQRKAADEGK
                     SCIVLIVFDGMDWQTSWAAAIWKGKKVGYREGRGTGLQFQDYRGCSTDFGYFVTSPFV
                     EGTSVDVNQQQIATAGNLRGGYNAGFGGSAPWSSIRDPIYPIGKGANLKHAYTDSAAS
                     ATSLTCGIKTYNDAINVDAFGRQATPIARQLQQEGFAVGVVTSVPISHATPACAYANN
                     VHRDDYQDLSRDLLGLPSISHYPAPLPGVDVLLGAGWGETKDKDGSQGRNFVPGNRYL
                     TASDLEEIGAADQGKYHVCQRTAGKPARSILTEAVASATSNHRRLFGFFGVKGGHLPF
                     QTADGKFDPTISVGQNPQAEVYSPADISENPTLAELSVAALDVLSSRANRMWLLVEAG
                     DVDWANHQNNIDNSIGAVLSGDAAFQAVAAWIEKNVGWEKSVVLLTADHGHYLVLEQP
                     EVLAESSAE"
BASE COUNT          771 a         1246 c         1190 g          804 t
ORIGIN      
        1 gagaattcgg ctttgacttg cgtcaggacc ttcgttgagg tcacgtacac cgacgagtac
       61 cgatactgca ccgtcacgtg gggaatcaat tgagatgtgt tctcgggaac tccctgggcg
      121 gtggcccagt tgggcaccac aggcagattg ccgtacgaca cgcccagatc attccagcag
      181 ttctgcaggc tctgctccac cgtcgattgc gagagcttgc caatggcgtc aaaagtgctg
      241 tcgtcgttca tcgaagcgga gaaatccagc acgagcacaa tatcgcgggg ctgatattgg
      301 gccgtcgatt gagcgtacag cgtgaagtcg tcgtagccga tcacgcgggc gaagaagaac
      361 ggcaggtttg gataggtcat cgagaccttg atgcccgacg gcatgacctg cgtctcctcg
      421 aaggcctgcg tcgcggggtt ccagttgccg atcttcgtct cgtagtcctc ggagtggtcg
      481 gtgtcgaatt cgaccaccgc gttggcaaac accgcttcgt cgatcgtgcc cacgtccgag
      541 ccgaccggat tgcggatcaa atactccttg gccgcggctt cggcttcggg aatgccgttg
      601 atcaattcgc cggcgccggc gagagcagcc gcatcgaccg cccgctggag ctgcgcctgc
      661 atcacgtaga tgtagccgac gtccaccgca agtgccacga agcacatcat gatgaccatc
      721 aggaacgcgc ttagcacgat gatattgccg cgacgggccg ggcgattccg gcggcctgca
      781 cccttttcac cccgcgtgca tgcctgtgtt gcgaacatga gatgtctccc ctttgccggg
      841 aaatgccgtg ggcccgatcg gggccgatat ggacgagagt tgaacgtaat ggctcccaag
      901 agcactgcgg attgccgcaa gggctttggt cagccaatag caaccatagg tcaagaaacg
      961 ccccccggca aaaaaatctg cacctcccct gtgaccagcg aaatccggct gatgcgtgcg
     1021 agtttttcag cccgcaccaa acccggaggc ccggctgtag aggttctcct tggtcgccca
     1081 cagacccgcg ctcggcgtgg gaatgaaata aatccgctcg cccgcgactt cgttgaatcc
     1141 ttcctggagc ttgtcgatcg gataccgccg cagcgtttcg gccaggtccg cgtattggaa
     1201 atggacctgc tcgatatcgg atttcgataa catgccgggg gcgtacgtga tgcgaaaccg
     1261 cccctcgctg ctgccgtgga tcaggtgcgc ggtggcgtgg gccaggtcct gcaggtcggc
     1321 attctgccgg tacaactcca tcactcgcgg cgtcccgcaa tagccatatt tgcggatcaa
     1381 agcgtccacg tcgggctgct cgccgaagcg tttgaggccc ggcgcgatga tgacgagctc
     1441 gccgccatcg gccagggcca tccgcgtgcg atagaccgcc ttgttcgcaa cccacgtgct
     1501 gaaaaactcg tccccctgca tcacgcaaac gaccttcttc agcggctcgt cgagcgcggt
     1561 gatgttctgc tcgcgcgcct gccgcgccgc ggccagatac gtctccagat cgtcgcccac
     1621 gaagacgccg gtatggacga gttggccggc tgcatcgcgg gccagcacaa cctgcgcgta
     1681 aaagtccggc agtccgctca agaaccgctc ctcggcccag ttgaagcact gccgcacggg
     1741 cgtcagcagg ttccccaggt tgttttcgat cccgcagcag gcagccgcca tgtgcgcggc
     1801 acaaatcagc tcctttccgc ccagcccgat gaaatagttc ttgttgtgat tcgcaaagcc
     1861 cagcacctcg tgcggaacga cgtggccaat gttgatcacc aggtcccacg gctcccgcag
     1921 gagcatcgaa ttcagccaca ccgggaactt ccaatccgcc gctccgcccg tcgcttcctt
     1981 gacgacctct gccggcacgt cccccaggta tgtgcagccg ccgcgccagt cgtgcggatg
     2041 aatccgctcg tgcggaatgt gcccaaacat ctgggcgttc tgctcgcgag tgtgcggctc
     2101 gtgctggccg agcgtcggaa tcacgtggac ctcggccgaa tctgccagca gcctgtaaaa
     2161 gcactccgta atccatccgc tccccgagtg catccgcgta atatcggggg gcaggagcag
     2221 gacgcgccgc ggagaggcac aaattcgccg cttcgcctcc gccagcgccc tctccactag
     2281 cgcctcaacc cgctggcgcg agatcgccgt gtctgactct gtaaaccaag gcatatctag
     2341 ttctgctgta ctaaagcggt tgccgctggg actctcgtgg tgccaggaac ttgcggcgtt
     2401 tgtggtgata atggcgggcg aactgtgcaa aatgtgatct cgcccgcgga ggccattcat
     2461 tccgcagacg actccgcgag cacctctggc tgttccagca ccaggtagtg gccgtggtca
     2521 gcggtgagca gcacgactga cttttcccat cccacgttct tctcgatcca ggccgcgacg
     2581 gcctggaagg ccgcatcacc gctaagcaca gcgccgatcg agttgtcgat gttgttctga
     2641 tgattggccc aatcaacatc cccggcttcg accaagagcc acattcgatt ggcacgtgag
     2701 gagagcacgt cgagtgccgc aacggatagc tcggccagtg tgggattctc agaaatgtcg
     2761 gccggcgaat agacctcagc ctggggattc tggcccacgc taatcgtcgg atcgaacttt
     2821 ccgtctgccg tttgaaaggg caggtgccct cctttgacgc cgaagaatcc aaacaggcgg
     2881 cgatgattgg acgtggcgga tgcgacggct tcggtcagga tggagcgagc cggcttcccc
     2941 gcggtccttt gacatacgtg gtatttccct tggtccgccg cgccgatttc ctccaggtct
     3001 gatgccgtca ggtagcggtt tccaggcacg aagttgcggc cttgtgaccc atccttgtcc
     3061 ttcgtctcgc cccagcccgc ccccaaaaga acgtccacac cagggagcgg tgcggggtag
     3121 tgtgaaatag aaggcaagcc gagcaggtcg cgggagaggt cctgatagtc gtctcggtga
     3181 acattgttcg cataagcgca ggctggggtt gcatgactga tcggcacgct ggtcacgact
     3241 cctacggcaa atccttcctg ctggagctgc cgggcaatcg gcgtggcctg acgaccgaag
     3301 gcgtcaacat tgatcgcatc gttgtaagtc ttgatgccgc aggtgagcga tgtcgcagaa
     3361 gcggccgagt cggtgtaggc gtgtttcagg ttggcccctt ttccaatcgg gtagatcgga
     3421 tcacggatgg aactccacgg agcgcttcct ccgaatccgg cgttgtaacc gccgcggagg
     3481 ttgcccgcag tcgcaatctg ttgttgattg acatcgaccg aggtcccttc gacgaaggga
     3541 ctcgttacga aataaccaaa atcggtgctg cagccacgat aatcctggaa ctgaagcccc
     3601 gtgccccggc cctctcgata gccaaccttc ttccccttcc agattgccgc cgcccacgat
     3661 gtttgccagt ccatgccgtc aaacacgatc aggacaatgc aggactttcc ctcgtcggcg
     3721 gccttccgct ggaggcgata tacatccgtc tggtcgaagt actcggcgga gggattcaat
     3781 gtcccctccg gctcgtagcc gtaaagggcc cgaatccggt cgagatcacg ataaaggctg
     3841 tttgccccgc gcacggtgga aagatccatg ccgaacgcat agagcggaat gagtcgattc
     3901 gagtgcgacg tccaggatga gtatttatcg ggatttggtc cccaaaggcc ccaggtggcg
     3961 ctaccctgtt ggacggccag gctttggagc tgccgaacgt cttccgctcg c
//
LOCUS       DASUGB010000948         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_246323, whole genome shotgun sequence.
ACCESSION   DASUGB010000948 DASUGB010000000
VERSION     DASUGB010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246323"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFV87_17970"
     CDS             <1..266
                     /locus_tag="VFV87_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105715.1"
                     /translation="PLRSATFARLGCGFLLLSVLISCVLLVFNGLIITNVYAASAATL
                     PEPLRDRRLSQAIVFLGPVLLLVVQWWAFDVTVDWLLPTRRRK"
     gene            complement(291..1196)
                     /locus_tag="VFV87_17975"
     CDS             complement(291..1196)
                     /locus_tag="VFV87_17975"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5105716.1"
                     /translation="MSRALVLSCFAVLVAMPALPADPPADDGKLRIVVFGAHPDDAEY
                     KAGGTAAKWARLGHHVKLVSVTNGDIGHWQMAGGPLAQRRAAESAAVAKKLGVASQVL
                     DIHDGELVPSLENRRLITRVIREWKADIVIAHRPWDYHPDHRYVGVLVQDAAFMVTVP
                     FICPDTPVLKRNPVFLYSSDGFQKPYPFQPDVAVSLDDVFDQKLDAIHELTSQIYEGG
                     ASGSEEYVRRIPPASDDAGRRAWLKDLWTDRQSREATRARAALTQWYGEERGKAVKYA
                     EAFEICEYGRRPSPDEIRKLFPFFD"
     gene            1377..1742
                     /locus_tag="VFV87_17980"
     CDS             1377..1742
                     /locus_tag="VFV87_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105717.1"
                     /translation="MDSRAPTTTDVSRALAAEDLRCGDYVAILHEVVEWPSFFWCAAD
                     PHLLPPDQPVRLATRSSEAGTPLKVKAISLPFVLVKKPCGEHLALDVRQHRLVRLSAD
                     YAREAWKTMSKQRPRRSLI"
     gene            complement(1811..>3214)
                     /locus_tag="VFV87_17985"
     CDS             complement(1811..>3214)
                     /locus_tag="VFV87_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105718.1"
                     /translation="GAGFTGVALAGLLGDDFFARQSLAADGQTRFVNPLAPKQPHAPP
                     KAKSVIFLYMYGGPSHIDTFDYKPTMVGMDNKTIAVKTFGRGGHKNQGRIVEPRWKFK
                     QYGECGKWVSDLFPNLSRHVDDIAFVHSMTADSPIHGSAMLQMNTGKVLSGSPCLGSW
                     VNYGLGSENENLPGFVVMLDPRGGPISGAKNWSSGYMPAHFEGTIFRSQGEPILDLAR
                     PGGMSEAAQRRLLDTLREYNDDHAAAHGHNQALASRIASYELAFKMQATAPEATNLAD
                     EPASIQELYGLNDQRCATVARQCILARRLVERGVRFVQIYSGGAHNDDNWDAHGDLVA
                     NHNLHAGETDRPVAALLADLKQRGLLDSTLIVWGGEFGRQPTAEYAEGTGRDHNAYGF
                     TMWLAGGGIRGGISVGKTDEIGSAAVERPFHVKHLHATMLQQLGLDPNRLTYFYGGLD
                     QKLVGVEGAEPIHELIA"
BASE COUNT          476 a         1054 c         1080 g          604 t
ORIGIN      
        1 cccctctccg ttctgccacg ttcgcccggc tcggctgcgg ctttttgctg ctttcggtgc
       61 tgatttcgtg cgtgctgttg gtgttcaatg gactgatcat caccaacgtg tacgccgctt
      121 ccgccgcgac tctgcccgag ccgctccgcg accgccgcct gtcgcaagcg atcgtcttcc
      181 tggggccggt cctgctcctg gtcgtgcaat ggtgggcctt cgacgtgacc gtcgattggc
      241 tactccccac ccgccgccgc aagtagcggg ccgcggcggc cgagccccgt tcaatcgaag
      301 aacgggaaca gcttgcgaat ctcgtcgggg gacgggcggc ggccgtactc gcagatttca
      361 aatgcctcgg cgtatttcac cgctttgccc cgctcctcgc cgtaccactg cgtgagggcg
      421 gcgcgggccc gcgtcgcttc gcggctttgg cggtcggtcc acaggtcctt gagccaggcg
      481 cggcggccgg cgtcgtcgct ggccggagga attcgccgca cgtactcctc gctgccgctg
      541 gctccgcctt cgtaaatctg agacgtcagc tcgtggatcg cgtcgagctt ctggtcaaac
      601 acgtcgtcca gcgagacggc gacgtccggc tggaacgggt acggcttctg aaagccgtcg
      661 ctggaataga ggaagaccgg gttccgcttg agcaccggcg tatcggggca aatgaagggc
      721 acggtgacca tgaaggccgc gtcctgcacc agcacgccga cgtagcggtg gtcgggatga
      781 tagtcccagg gccggtgcgc gatgacgatg tcggccttcc actcgcggat gacgcgggta
      841 atgagccggc ggttttcgag cgatgggacg agctcgccgt catgaatgtc gagcacctgc
      901 gacgctacgc ccagcttttt cgccaccgcg gccgattccg cggcccgccg ctgggccagc
      961 ggcccgccgg ccatctgcca gtgcccgatg tccccgttgg tgacgctcac cagcttcacg
     1021 tgatgcccga gccgggccca cttggccgcc gtgccccctg ccttgtactc ggcgtcatcg
     1081 ggatgtgccc caaacacgac aatccgcagc ttgccgtcgt ccgccggcgg atcggcggga
     1141 agggcgggca tagcaaccag cacagcaaaa cacgataaca ccagggcgcg cgacatggca
     1201 ggactcccat cgaagaagca tgaatcagcc gattcgtcgt cctgattcaa cttggacggc
     1261 ggggggttgt caatgaatgc gttgaatgcc gcaggcgaaa tggatttttg tccgaaccct
     1321 tcgggcgact ggcgcgtcgc tgccatttgc ggccgatttt gtttcgccgc tgccttatgg
     1381 attctcgtgc cccaacgacg acggacgtat ccagggccct cgcggccgag gacctgcggt
     1441 gcggagacta cgtggccatt ttgcacgagg ttgtcgagtg gccgtcgttc ttttggtgcg
     1501 ccgccgaccc gcacctgctg ccgccggacc agccagtgcg gctggcgacg cgctcgagcg
     1561 aggccgggac gccgctcaag gtgaaggcga tctcgctgcc tttcgtgctg gtcaaaaagc
     1621 cctgcgggga gcacctggcg ctcgacgtgc ggcagcaccg gctggtgcgc ctgtcggccg
     1681 actacgcccg ggaggcctgg aagacgatga gcaaacagcg gccgcgtcgc tcgctgattt
     1741 agcgcggcag gacagccctg cgcgaccact ggtcgcggct gtgcatttcc tgcgcgacca
     1801 ctggtcgcgg ttaggcaatt agctcgtgga ttggctcggc cccttcgacg ccgacgagtt
     1861 tctgatccag gccgccgtag aaatacgtca ggcggttggg atcgaggccc agttgctgca
     1921 gcatcgtggc gtgcaggtgc ttcacgtgaa agggcctctc gactgcggcg ctgccgatct
     1981 cgtcggtctt gcccacgctg atcccgccgc ggatgccgcc gccggccagc cacatcgtga
     2041 agccgtaggc gttgtggtcg cggccggtcc cctcggcgta ttccgcggtc ggctggcggc
     2101 cgaattcacc cccccagacg atcagcgtgc tatcgaggag gccccgctgc ttgagatcgg
     2161 ccaagagcgc ggcgacgggc cggtcggtct cgccggcgtg caggttgtgg ttggccacca
     2221 ggtcgccatg ggcgtcccag ttgtcgtcgt tgtgcgcccc gccggagtag atctgcacga
     2281 accgcacgcc tcgctcgacc agccgtcggg ccaggatgca ttgccgggcg acggtggcgc
     2341 agcgctggtc gttcaggccg tacagttcct ggatgctggc cggctcgtca gccaggttgg
     2401 tcgcctccgg ggcggtggcc tgcatcttga acgccagctc gtagctggcg atccgcgagg
     2461 cgagggcctg gttgtggccg tgggccgccg cgtgatcatc gttgtactcg cgcagtgtgt
     2521 cgaggagccg gcgctgggcc gcctcgctca tgccgccggg ccgggccagg tcgagaatcg
     2581 gctcgccttg cgagcggaag atcgtcccct cgaaatgagc cggcatgtag ccgctgctcc
     2641 aattcttggc cccgctgatc ggcccgccac ggggatcgag catgacgacg aatcccggca
     2701 ggttctcgtt ttcgcttccc aggccgtaat tgacccacga acccaggcag ggactgccgc
     2761 tgagcacctt gcccgtgttc atctgcagca tggcggagcc gtggatcggc gagtcggcgg
     2821 tcatcgagtg gacgaacgcg atgtcgtcca cgtggcgcga caagttggga aacaggtcgc
     2881 tgacccactt gccgcactcg ccgtactgct tgaatttcca ccgcggctcg acgatgcggc
     2941 cctggttctt gtgcccgccg cggccgaacg tcttgaccgc gattgtcttg ttgtccatgc
     3001 cgaccatcgt cggtttgtag tcgaacgtgt cgatgtggct cgggccgccg tacatgtaca
     3061 ggaagatcac gctcttggcc ttgggcggag catggggctg cttgggcgcc agcggattga
     3121 cgaatcgcgt ctgcccatcg gcggccagcg actggcgggc gaagaagtcg tcgcccaaca
     3181 ggccggccaa ggcgacgccg gtgaacccgg cgcc
//
LOCUS       DASUGB010000949         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1912000, whole genome shotgun sequence.
ACCESSION   DASUGB010000949 DASUGB010000000
VERSION     DASUGB010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912000"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VFV87_17990"
     CDS             complement(<1..2056)
                     /locus_tag="VFV87_17990"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5105719.1"
                     /translation="MSTAAFPIPQTVPAARRDLLACAVEFRGQRYWTIKDPVSLRYYQ
                     LRDEEHFVLGLLDGRHSLAEIVERFERRFAPRRLRSGDLAGFLLMLHREGLVVASAWG
                     QGEHLIERRQQKRREKILAQWMNVLAIRLPGVNPNRLLDFLVPRFAWLFSLWTLLIVT
                     TLIGTALLVAAVNFGTLIARLPTFHEFFGPGNILWLAAALALVKVLHELGHAVACKSF
                     GGECNRLGVMLLVFVPALYCDVSDAWMFARPLRRIAVSAAGMAVELVLAAVAAVLWAV
                     TEPGWFNALCLNILFVCSVGTLFINGNPLLRYDGYYILADLLGTPNLSQQASAALRRW
                     SARLFAGVDFDQPKLLAEPGWVLLLGYGLASTIYRGLVILAVLWLIQAALAPLGLAVV
                     AQLVTLLVLGALASGPARAVVRFLRDPGKRSRIQPGRALASMMVLAGVSAAVLTIPLP
                     SRVTAPVVLRPEGARQVYVTTAGMLESSVAGGDVVSEGQTLARLHNRTLEVEAVELQS
                     RLKQQRLHVQQLEIRRHDVPALGDQLPAAQEALQDLQEQFELKRRDLDRLVLTAPIAG
                     VVIPPPAVAGPSDQDELPGLTGTPLDAENQGCRLEAGSSFCQIGDPERLQAVIIVNEA
                     DVQRLRPGQLVRLNLQQTPGEIRTGRVREVARVNAGDVPPELITANLLPIRRGASGQP
                     GPI"
     gene            complement(2069..2953)
                     /locus_tag="VFV87_17995"
     CDS             complement(2069..2953)
                     /locus_tag="VFV87_17995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5105720.1"
                     /translation="MQRFVKSWLMAGCWGLCGAVAAADIEVESVVVNLVADVEVPAKE
                     AGALVEIPAAEGHRVEAGAVLARIDDRDAALEVTRAEIALEHARQQAANDVKLRLARQ
                     SLKLAELELKRAEDANRELEKVVSATQIEKLRIEVEKSQLEVELALKDLAAAERAVAA
                     AKNELLIAQRAVERRTVVAPMGGTVVDVRRRPGEWLEPGQTVVRLVRDDRLRAEGFVL
                     VDTLTTEVIHQPAELIVALPGEKSATFRGTVTFVSPEVNPINRQVRVWAEFDNADGRL
                     RSGIPAKLILKPATSATP"
     gene            complement(2985..4319)
                     /locus_tag="VFV87_18000"
     CDS             complement(2985..4319)
                     /locus_tag="VFV87_18000"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5105721.1"
                     /translation="MLRFDQSRAKSLAVSGVDQLDRRSAQVASLERLASAVARSGEPV
                     EWPRPPLDLPPQIDQALQEHLDIAHCRRLIVAPLCPRAAKDSGFEPAIRGVLIAESFD
                     ERKEELGFRERTGDVARHGGAALLAALEHHDLPLLPLQVKLGRLFRRLQQRRIAVALF
                     LVIALAVIGLLVAIPADLTIEARGTLQPQVRKHLFAPVEGIVEKVQADHGAHVAQGDP
                     LLLLRDPRLELDDSRVSGELQTAQARLIAVQTQRSSRSTSPDTREQGRELAAEEEQLK
                     EQVRGLEAQHALLRKLRGELSIASPIDGVVLTWNTTELLQDRPVKQGQRLVTVADPTG
                     PWVVELRIPDADLGHVLRAQEQSGRELPVSFLLATDPAITYRGQIERVAVATDVAEDS
                     PAALTIVRFTGQPPAGALAGTSVTARIHCGAKPIGYVWLHDLIDAIRTQVLF"
BASE COUNT          862 a         1449 c         1427 g          756 t
ORIGIN      
        1 cgataggacc tggttggccg ctcgccccgc ggcggatggg cagcagattg gccgtgatca
       61 actcgggagg cacatccccc gcattcacgc gcgcgacctc ccgcactcgg cccgtgcgga
      121 tctcgccggg cgtttgctgc aggttcaaac gcaccaactg gccgggccgc aaacgttgca
      181 cgtccgcctc gttgacgatg atcaccgctt gcagccgctc cggatcgccg atctggcaga
      241 atgaggagcc ggcctcgaga cggcagcctt gattttcggc atccagtggt gtgccggtca
      301 aacctgggag ctcgtcctga tccgacggcc cagcgacggc cggcggtgga atgacgacgc
      361 cagcgatcgg cgcggtgagc accaggcggt ccaggtcgcg ccgtttcagt tcgaattgct
      421 cctgcagatc ctggagggcc tcttgagccg ccggcagttg atcgcccaga gccggcacat
      481 cgtgacgccg aatttccagt tgctgcacgt gcaggcgctg ctgcttgagc cggctctgca
      541 attccaccgc ttcgacttct aatgtgcgat tgtggagccg ggccagcgtt tgcccctccg
      601 atacgacgtc accgccggcc acggacgatt ccaacatgcc ggccgtggtg acatagacct
      661 ggcgcgctcc ttccggacgc agcaccaccg gcgcggtgac gcgcgaggga agtggaatcg
      721 tgaggacagc agcgctcacc ccggccagca ccatcatcga agccagcgcg cggcccggct
      781 ggatgcggga gcgtttgccg ggatcgcgga gaaatcgcac gacggctcgc gccggcccgc
      841 tggccagcgc gccaaggacg agaagggtga cgagctgggc gacaaccgcc agccccagcg
      901 gcgccagggc tgcctggatc agccacagca ctgccagaat aaccagccca cgataaatgg
      961 tcgaggcaag tccataaccc agcaacagca cccatccggg ttcagccagg agctttggct
     1021 ggtcgaaatc tacgccggca aagagccgtg ccgaccagcg gcgcagcgct gccgacgctt
     1081 gttggctcag gttcggggta ccgagcaaat cggcgaggat gtaatagccg tcgtagcgca
     1141 ggagcggatt tccgttgatg aacagcgtgc cgacagaaca gacaaacaaa atgttcaggc
     1201 acagcgcgtt gaaccatccg ggctccgtca ctgcccacag caccgcggca acagcagcca
     1261 ggaccaattc caccgccatc cccgcggcac tcaccgcgat tcgccgcaag gggcgggcga
     1321 acatccaggc gtcggacacg tcgcaataca gcgcgggaac gaacaccagc agcatgaccc
     1381 ccaggcgatt gcactcgccg ccgaaagact tgcacgccac ggcatgaccc aattcatgca
     1441 gtaccttgac gagtgccagc gcggcagcca gccaaagaat gttgcccggc ccgaagaatt
     1501 cgtgaaaggt tggcagccgg gcaatgagcg tgccgaagtt gactgctgcg accaacagcg
     1561 ccgtgccgat cagcgtcgtc acgatcaata gtgtccacag cgagaacagc cacgcgaacc
     1621 tcgggacaag gaaatcgagc agccgattcg gattgactcc cggcaaacga atcgccagga
     1681 cgttcatcca ttgggccagg atcttctcgc ggcgcttctg ctggcggcgc tcgatcagat
     1741 gctctccctg accccaggcc gaagcgacga ccaatccttc gcggtggagc atcagcagga
     1801 acccggccag atcgccggag cgaagacgcc gcggagcgaa tcgccgctca aagcgttcga
     1861 cgatttccgc cagcgaatgt cggccgtcga gcagccccaa cacgaagtgc tcctcatcac
     1921 gaagctggta atagcgtagc gatacaggat ccttgatcgt ccagtagcgc tggccgcgga
     1981 attccaccgc gcaggccagg aggtcgcgcc gggcggcagg aaccgtctgc ggaatcggga
     2041 atgcggctgt ggacattaag gcagaaggtc agggtgtggc cgaagtagcg ggcttgagga
     2101 tgagcttggc ggggatgccg gagcgcagcc ggccgtcggc attgtcaaat tccgcccaca
     2161 cgcgcacctg gcgattgatg ggattgacct ccggcgaaac gaacgtgacg gtgccgcgga
     2221 atgtcgccga tttctcgccg ggaagtgcga cgatcaactc ggccggctga tgaataacct
     2281 cggtcgtcag cgtatcgaca agaacaaagc cctcggcccg caaacggtca tcgcgcacca
     2341 gccgcacgac ggtctgtcct ggttcgagcc actctcctgg ccgccgccgg acatcgacaa
     2401 ctgtgccgcc catcggcgcg acaacggttc ggcgctccac ggcccgctgg gcaatgagta
     2461 actcattttt cgcggcagcc accgcccgct cggcagccgc gaggtctttg agggccagtt
     2521 cgacttcgag ctgagatttc tccacctcga tgcgaagttt ttcaatctgc gttgctgaga
     2581 cgaccttttc cagttcgcga ttggcgtctt ccgcccgttt cagctccagc tccgcaagct
     2641 ttagcgattg ccgggcaaga cgcagcttca catcgttggc ggcttgctgc ctggcatgtt
     2701 caagggcgat ttcggcgcga gtcacttcca aggctgcatc gcgatcgtcg atccgcgcga
     2761 ggaccgctcc ggcttcgaca cggtgtccct cagccgcagg tatttcgacg agcgcgccgg
     2821 cttccttggc gggcacttcg acatcggcca cgagattgac cacgacggac tccacttcga
     2881 tgtccgccgc cgccactgcg ccgcacagac cccagcagcc ggccatcagc cagctcttaa
     2941 cgaatcgctg cattgcagag gctcctgttt gtggtgcgca gatttcaaaa gaggacttgg
     3001 gtgcgaatgg cgtcgatcag gtcgtgcagc caaacatagc cgatcggttt ggcgccgcag
     3061 tgaatgcggg ccgtgacgct ggttccagcc agagcgcccg cggggggctg gccagtgaag
     3121 cggacaatcg tcagagctgc cggcgagtct tccgccacgt cggtcgccac ggcgacgcgc
     3181 tcgatctggc cgcggtacgt aatggctgga tcggtcgcca acaaaaatga gacgggtaat
     3241 tcccgcccgg actgttcttg cgctcgcaag acgtgcccca gatcggcatc gggaatccgc
     3301 agctcgacaa cccagggacc cgttgggtcg gcaacggtaa caaggcgttg cccctgcttc
     3361 acgggacggt cttgcaagag ttcggtcgta ttccaggtca ggacaacgcc gtcgatcggg
     3421 ctggcgatgc tcagctcgcc gcgcagcttg cggagcaacg cgtgttgtgc ttccaggccg
     3481 cggacctgtt ctttgagctg ctcctcctcg gcggccaatt cgcgaccttg ttcgcgggtg
     3541 tccggagagg tactgcggct ggaacgctgc gtttggacgg cgatcaaccg agcctgggcc
     3601 gtttgcagct cgccgctgac gcggctgtcg tcgagttcga ggcgcggatc tcgcagcagg
     3661 aggaggggat cgccctgcgc gacatgggcg ccgtggtcgg cctggacctt ctcgacaatg
     3721 ccttccacgg gggcgaagag gtgcttacgg acttgaggtt gaagcgtgcc gcgggcttcg
     3781 atcgtcaggt cggcgggaat cgcgaccagc aagccaatca cggcaagggc gattaccaga
     3841 aacagcgcaa cggcgatccg ccgttgctgc agacgccgga acaatcgccc cagcttgacc
     3901 tgaagcggca acagcggcag atcgtggtgc tccagtgccg ccaagagcgc cgcaccgccg
     3961 tgccgggcga cgtcgccggt gcgctcgcga aaacccagct cttccttccg ttcatcgaac
     4021 gactcggcaa ttaagacgcc acgaatcgcg ggctcgaaac ctgaatcctt cgcggcccgc
     4081 gggcagagcg gcgcgacgat gagcctgcgg cagtgggcga tgtcgaggtg ctcttgcagg
     4141 gcctggtcaa tctggggcgg caggtcaagc ggcggacgcg gccactccac cggctcgccg
     4201 cttcgagcga cggcgcttgc caggcgctcc aggctggcaa cctgcgcact gcggcgatcg
     4261 agttgatcga cgccgctgac ggccagcgat ttcgcccggc tctgatcaaa gcgaagcacg
     4321 cttagccgat cgcaaccaat ccaattccga ccgtcattgg ctatcgcata cgcggtggcc
     4381 gcgagatcca ggctgtcgtg aattcgcaga acgaattcat cgaacagctg ccggtcggcg
     4441 ccatgttgcc ggagtgcggt caattcgcgc ttccggttga agtcagtcgc tatt
//
LOCUS       DASUGB010000950         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1495182, whole genome shotgun sequence.
ACCESSION   DASUGB010000950 DASUGB010000000
VERSION     DASUGB010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495182"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="VFV87_18005"
     CDS             <1..2269
                     /locus_tag="VFV87_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105722.1"
                     /translation="DSVLGTPLYMSPEQAQLNNLDVDTRSDIYSLGVLLYELLTGTTP
                     LEKQRFREAAWDEVRRIIREEEPPRPSTRLSSTDTLPSLAACRQTEPASLTRLIRGEL
                     DWIVMKALEKDRTRRYETANGFAMDVQRYLAGEPVLAVPPSTGYRLLKFARRNKRGLT
                     TAACVVVGALIAVGAVGWAVRDRVARQQAAFEEQEARQAKLEDEIARALEDVASSYQQ
                     DKLPDAMAAMKRAEALLVSGDSREELRQRVRRWRVDLDTVDRLEALYLERYEVKKWYG
                     DWKGADRAYLEAFRRYGLDLEAAEPASAIMTIRASAVRDRLVAGLDDWLGVARGVNRT
                     VGERLLVVVRDADPDPWRNRLRVVFWRNDIQAVRRLAEDPSVSSQPPANVLLLVALLN
                     REDDNPLAIEVLRKAQRRFPANFSINYVLGSKIMASDDPTLFSDAAGFLRAAAALRPR
                     SAIVYRSLGSSFRLLEKADDAEAALREAVRCDPDYALGRNNLADILMFRDRLDEAETE
                     VRRAINLDPDLGIAHFTLGEIMERSGKPAEAAAQFRQALRLDLNVAACFYCTARLLLL
                     DGDTDEYRPACDRLQKRYAQDQRRWTLYFLARTCVLAPHATPDPAQAVRLAELAVSAE
                     PKWAHNLHTLGLAHYRAGQFEEAVRRFHEAIDANPGWTGQVVNWLGLALAYHCQGQTD
                     EARRWYDKALEWIDRVAPNGNRDSLTPPPGVHDHEWLASRLLRREAELLMKVSMPIDI
                     KAKLGADRPNTREKR"
     gene            2301..>2402
                     /locus_tag="VFV87_18010"
     CDS             2301..>2402
                     /locus_tag="VFV87_18010"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5105723.1"
                     /translation="MSDITQLLDAARGGDKQAVAELLPLVYEELRRLA"
BASE COUNT          443 a          735 c          784 g          440 t
ORIGIN      
        1 cgattcggtg ctgggcacgc ccttgtatat gtcgcccgag caggcgcagc tcaacaacct
       61 cgatgtggac acgcgctcgg acatctactc gttgggcgtg ctcttgtatg aactcttgac
      121 gggaacaacg ccactggaga aacagcgatt cagggaggcg gcgtgggatg aagtccgccg
      181 cattatccgc gaggaggagc cgccgcgccc gagcacgcgt ctcagttcaa ctgacaccct
      241 gccctctttg gccgcttgcc ggcagacaga accggccagt ctgaccaggc tgattcgcgg
      301 cgagctcgac tggatcgtga tgaaggccct ggagaaggac cgcacgcgcc gctacgagac
      361 cgccaacggc ttcgccatgg acgtgcagcg ctacctggcg ggggagcccg tgctggccgt
      421 gccgcccagc acgggttatc ggctgctcaa attcgcccgg cggaacaagc ggggcttaac
      481 aactgccgcc tgtgtcgtcg tgggggcact cattgccgtt ggcgccgtcg gctgggctgt
      541 tcgggatcga gtagcgcgcc agcaagcagc attcgaggaa caggaggcgc gacaggcgaa
      601 actcgaggat gagatcgcaa gagcccttga ggacgtcgca agctcgtacc agcaggacaa
      661 gcttcccgat gcgatggcgg caatgaagcg cgccgaggcg ctcttggtca gcggcgactc
      721 tcgcgaggag ctgcgacagc gtgtgcgccg ctggcgggtc gatctcgata cggtggatcg
      781 cctggaggcg ctttaccttg agcggtacga agtcaagaag tggtatggtg attggaaagg
      841 cgcggaccga gcttacctgg aagcgttcag gcgctacggt ttggacctgg aggcggcgga
      901 accggcctcg gccatcatga ccattcgggc atcggccgtc cgcgatcggc tcgttgccgg
      961 gctcgatgac tggttagggg ttgcacgagg tgtcaatcgt accgtcggcg aacgattgct
     1021 ggtcgtcgtc cgtgacgccg acccggaccc ctggcgcaac cgcctccgcg ttgtgttttg
     1081 gcgaaacgac attcaggctg tccggcgcct ggccgaggac ccaagcgtct caagccagcc
     1141 gccggccaac gtattgctct tagtggccct cttgaaccgg gaagatgata atccgctggc
     1201 aatcgaggtc ttgcgcaagg cgcaacggcg ctttccggcc aatttttcga tcaactatgt
     1261 attaggcagc aagatcatgg cgagcgacga cccgacgctt ttcagcgacg cggccggctt
     1321 tttacgcgct gccgcagcgc tgcgcccccg cagcgccatc gtctatcgtt cgctggggtc
     1381 gagctttcgt ctgctggaaa aggctgatga cgcggaagcg gccttgcgcg aagcggtgcg
     1441 ctgcgatccg gattatgctc tgggccgcaa caatctagca gacattctta tgtttcggga
     1501 ccgactggat gaagcggaaa ctgaggttcg ccgggcgatc aacctcgatc ccgacctggg
     1561 catcgcccat ttcaccttgg gcgagatcat ggaacgaagc ggcaaaccag ccgaagccgc
     1621 agcccagttc cggcaggctc tccgcctcga cctcaacgtt gccgcttgct tctactgcac
     1681 ggcccgtcta ttgctcctgg atggggacac cgacgaatac cgccccgcct gtgatcggct
     1741 ccagaagcgt tacgctcagg accaacggcg atggacgttg tactttttgg cccggacgtg
     1801 tgtattggcc ccccatgcga ctcctgatcc ggctcaggcg gttcggctcg cggagctggc
     1861 cgtcagcgcc gagccgaagt gggcccataa cctgcacact ctcggtcttg cacattaccg
     1921 cgccggtcaa ttcgaagaag ctgttcggcg attccacgag gccatcgatg ccaatcccgg
     1981 atggaccggc caggtggtga actggctggg gttggcgttg gcgtaccatt gccagggcca
     2041 gaccgacgaa gcgcgtcgct ggtacgataa agccctggag tggatcgacc gggtcgcccc
     2101 gaacgggaac cgcgactcac tgacgccgcc gccgggtgtg catgaccacg aatggctggc
     2161 gagtcgtctt cttcgccgcg aggcggagct cctgatgaaa gtgtctatgc cgatagacat
     2221 taaggccaag ctgggggccg accgccccaa cacgcgggag aagaggtgac gacggttgac
     2281 ggatcggcta gggtctcact gtgtccgaca tcacccaact tcttgacgcg gcccgcggtg
     2341 gcgacaagca ggccgtcgcg gagttgctcc cgctcgtgta cgaggaactg cgccgactcg
     2401 cg
//
LOCUS       DASUGB010000951         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_662062, whole genome shotgun sequence.
ACCESSION   DASUGB010000951 DASUGB010000000
VERSION     DASUGB010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662062"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFV87_18015"
     CDS             complement(<1..585)
                     /locus_tag="VFV87_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105724.1"
                     /translation="MFIAATTGLLMSACANMGGLGNIGLGTPRADFTKAQADMAGCRG
                     AARSPGELTACSAKFGPALAQIAQDSFDQAGRAQDRATRINLHATAANAGWDSGTDAG
                     LQVADAAIQAGGGECDKIPPNEFTPARDCALLRVGPGFVAHMRTTALVDRIAAKPPAS
                     VTAAEKRQLNDASLRYVRNTFDFIESRRRQFEADP"
     gene            complement(599..2026)
                     /locus_tag="VFV87_18020"
     CDS             complement(599..2026)
                     /locus_tag="VFV87_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105725.1"
                     /translation="MDFVGSNPGILGVGGKGEKLIVALSANFRALLPDVDKKDGWKGR
                     EATHFFVERALAITLHAGLKTISENPDLLVEEEHHRELLQNVVEPFVTGFDKNPDNRP
                     QWIDVRDTLLGPVAEAALKTVQRHQDAFLGGKLSSNTAVRAVTAALLDTATAGDTGEL
                     FTSEGVVRLYRTALDLGVKNPRLFITATGEKSDVARDLLVRIGSAIRDAPPQFDKALV
                     ADVAIAALESLKGNVPGLLRIKDDAWRNVAGDLTTKVLGGLQKGIGTGDARAAFETMF
                     TPEETVALLSIVMKQVVQTPGMITGAQVSDEVQALMATIAGAISTRGSELLTPDDWIA
                     VAQTIAVEAARNPGRIIPLAGPPEQQLAGKILQVLLDAAAQSFPDGKRTNASLLFGQT
                     LREAAVIAIQAAAGNSGKAARNLEAFRNFVQILNNAAATNAGQIGAREWLRLFDNRIA
                     RVFAIGIPAGLTVASLVSELEQSHA"
BASE COUNT          330 a          670 c          697 g          385 t
ORIGIN      
        1 gggatcggct tcgaattgcc ggcgccggct ttcgatgaag tcgaatgtgt ttcggacgta
       61 ccgcaagctg gcgtcgttca actgcctctt ctcggccgcc gtcaccgagg cgggcggctt
      121 ggccgcgatc cggtcgacca aagccgtcgt tcgcatatga gcgacgaagc ccggtccgac
      181 ccggagcagg gcgcaatcgc gggccggcgt gaattcattc ggcggaatct tgtcgcattc
      241 gccaccgccg gcctggatcg cagcgtcggc gacctgcagg ccggcgtcag tgccgctgtc
      301 ccagccggcg ttggccgccg tggcgtggag attgatgcgc gtcgcccggt cttgcgcccg
      361 gcctgcctga tcgaagctgt cctgcgcgat ctgggcgagc gccggcccaa atttcgccga
      421 gcacgccgtc agttcgccgg gggagcgggc cgcgccgcgg cagcctgcca tgtccgcctg
      481 cgccttggtg aaatcggcgc gcggcgtgcc gagcccgatg ttgccgaggc cgcccatgtt
      541 ggcgcaggcc gacatcagca agcctgtcgt cgcggcaatg aacaagatcg gcagaggtct
      601 aggcatggct ctgctccaat tcggacacca gcgatgcgac ggtgagcccg gctggaatgc
      661 cgatcgcaaa gacccttgcg atgcggttgt cgaacagtcg tagccactcg cgagcgccaa
      721 tctgtccggc gttcgttgcg gcagcgttgt tgaggatctg aacgaaattc cgaaacgcct
      781 ccagattgcg cgcggccttg ccggaattgc cggctgcggc ctgaatggcg atgacggctg
      841 cttcgcgaag cgtctgcccg aacaggaggc tcgcattggt gcgcttgccg tccgggaacg
      901 attgagcagc cgcgtcaagc agcacctgga gaatctttcc ggcgagctgc tgctccggcg
      961 ggccggcaag cgggatgatc cggcccggat tgcgggctgc ctccaccgca atggtctgcg
     1021 cgacggcgat ccagtcgtcg ggcgtcagaa gctcggagcc gcgggtcgag attgcgcccg
     1081 cgatcgtcgc catcagcgcc tgcacctcgt cgctgacctg ggcgcccgta atcatgccgg
     1141 gcgtctggac cacctgcttc atgacgatcg aaagcagagc gacggtttct tcgggcgtga
     1201 acatcgtctc gaacgccgcc cgagcgtccc ccgtaccgat gcccttctgc agcccgccga
     1261 gtaccttggt tgtcagatcg cccgcgacat tgcgccaggc gtcgtccttg atgcgaagca
     1321 ggcccggcac gtttcccttc agcgactcca gggcggcaat cgcaacgtcg gcaacgagcg
     1381 ccttgtcgaa ttgaggcggg gcgtcccgga ttgcactgcc gatgcggacc agcaggtcgc
     1441 gcgcgacatc cgacttctca cccgttgctg tgatgaacag ccgtggattc ttgacgccga
     1501 ggtcgagggc cgtgcggtag agacgcacga caccttcgga tgtgaacagt tcgccggtgt
     1561 cgcctgctgt cgcggtgtcg agcagcgccg ccgtaaccgc gcgcactgct gtgttggacg
     1621 agagcttgcc gcccaggaac gcgtcctgat gccgctgcac tgtcttgagc gccgcttccg
     1681 cgaccggacc cagcagcgtg tcgcgaacgt cgatccattg cggacggttg tcggggttct
     1741 tgtcgaagcc cgtgacgaac ggctcgacga cattttgcag aagttcgcgg tggtgctctt
     1801 cctcgaccag caggtcgggg ttttcgctga tggtcttcag tccggcatgc agcgtgatgg
     1861 cgagcgcgcg ttcgacgaag aagtgcgttg cctcgcgccc tttccagccg tctttcttgt
     1921 caacgtcggg caagagcgcc ctgaaattgg cgctgagcgc aacgatcagc ttttcgccct
     1981 tgccgccgac gccgagaatc cctggattgg agccgacgaa gtccaaagca acgtcggtga
     2041 atgccaggac aaggcggagc gccggcgtgg gcggtccggt cc
//
LOCUS       DASUGB010000952         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_662080, whole genome shotgun sequence.
ACCESSION   DASUGB010000952 DASUGB010000000
VERSION     DASUGB010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662080"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFV87_18025"
     CDS             <1..780
                     /locus_tag="VFV87_18025"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105726.1"
                     /translation="SDDPNTRANLALEDLAPPRDRVGETQQNQFEIQRFLQQGFANKF
                     SGDAAKAHAASYAKAMRMVETQARKAFQLEEEKPELRDAYGRNRFGQGCLLARRLVER
                     GVAFCEVALSGTPNNGAGWDTHGNNFDQVRQLSEVLDPAWSTLMTDLRERGLLESTLI
                     IWMGEFGRTPKINQNQGRDHFPNAWSVVLGGAGIKGGQAIGDTGPSGEDVKDRPVKTN
                     DLYATIWAALGLDYTKENVSPEGRPIGIVDSGGTPVRELVG"
     gene            844..>2245
                     /locus_tag="VFV87_18030"
     CDS             844..>2245
                     /locus_tag="VFV87_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105727.1"
                     /translation="MTSTKRQSTARCLFLAACIAAAVLLAASAASAQPRSAVSTTTSG
                     TRAAPADQADGQRDVLVMLDNGPLHMRLHVALGGVALAQTRDEYVARLIESFDENKDG
                     KLSRDEAQKSPILRTKERPGAAQFLQSLKAQSNLIARDVRQRIDALGGELIAYRQDLA
                     SSQNDVEVFKLLDTDSSGLLDAAEMEAAAALIMLKDTDNDQCVSFEEFFPPPPPPDPM
                     LVAAGLAEQAPAPPMATIADMIRSATEPLIAHRLVKRYDKPDGATRRRDAMLSASELG
                     WSEERVAAIDADGNRLLDLGELANISQGAPDIEMSVDLRALDAAGGLLHVLSTVGERL
                     DDATRLDYAKISFDSAVVTFSHRNLDPVADAIDDAMQQFNLLDADANGYLSRDEVAER
                     IRFQRELFDLMDGDGDDKVFVDEMKAYVKARAEPAAGTCRVNVYDAGNGYFMALDTNA
                     DGRVSEREKRKAAQSLA"
BASE COUNT          451 a          701 c          706 g          387 t
ORIGIN      
        1 agcgacgatc ccaacacgcg agctaatctc gcgctggaag accttgcccc gccgcgcgac
       61 cgcgtcggcg agacgcagca gaaccagttc gaaatccagc gcttcctgca gcagggcttc
      121 gcgaacaaat tttccggcga tgcggccaag gcccatgcgg ccagctatgc gaaagcaatg
      181 cggatggtcg agacgcaagc ccgcaaggcg tttcagctcg aagaggaaaa gcccgaactc
      241 cgcgacgctt atggccgtaa ccgtttcggc cagggctgtt tgctcgcccg ccgtctggtt
      301 gagcgcggag tggcattttg cgaggtagcg ctatccggca cgcccaacaa tggcgccggc
      361 tgggatacgc acggcaacaa tttcgaccag gtgcgtcagc tttccgaagt gctcgatccc
      421 gcctggtcca cgctcatgac ggatttgcgc gagcgcgggc tcttggaatc aacgctcatc
      481 atctggatgg gtgaatttgg ccgcacgccg aaaatcaatc aaaaccaggg gcgcgatcat
      541 ttcccaaacg cctggagcgt cgtgctcggc ggcgccggca tcaagggagg gcaggcgatc
      601 ggcgacaccg gtcctagcgg cgaggatgtc aaggatcggc ccgtgaagac gaacgacctg
      661 tacgccacaa tttgggcggc actcggactc gattacacca aggaaaacgt ctcacccgaa
      721 ggccggccga tcgggatcgt cgatagcggt ggaacgccgg ttcgagagct tgtcggttag
      781 cacaggagtg gcccattcac gcattcgcag gatgggtcaa acgggatcgt ggggagtttt
      841 gccatgacca gcacgaaacg ccagtcaact gcgcgctgcc tgttcttggc ggcttgcatt
      901 gccgccgccg tgctgttggc ggcgagcgct gccagcgccc agccgcggtc agccgtttcg
      961 accacgacct ctggcacgcg ggcagcccca gccgaccaag cggacggcca gcgcgatgtg
     1021 ttggtgatgc tcgacaacgg gccgctgcac atgcgcttgc acgtcgcact gggcggcgta
     1081 gcgctcgcgc aaactcgcga cgaatatgtc gcccgattga tcgaatcgtt cgacgaaaac
     1141 aaagacggca agctatcgcg cgacgaggct cagaaatcgc cgatcctccg caccaaggaa
     1201 cgaccgggcg ccgcccagtt cttgcagtcg ctcaaggctc aatccaatct gatcgccagg
     1261 gatgtacgcc agagaatcga tgcgttgggc ggcgagctaa tcgcttatcg ccaggacttg
     1321 gcgtcgtcgc agaatgacgt cgaagtcttc aagctgctcg atacggattc gtccgggctg
     1381 ctcgacgcgg ccgagatgga agccgcagcc gcgctcatca tgttgaagga caccgataac
     1441 gatcagtgcg tcagcttcga agagttcttc ccgccaccgc cgccgcccga tcccatgctg
     1501 gttgccgccg gtctcgccga gcaggctcca gcgccgccga tggccacgat cgccgatatg
     1561 atccgcagtg cgacggagcc actcatcgca caccggttgg tgaagcggta cgacaagccg
     1621 gacggcgcga cgcgccgtcg cgacgccatg ctcagcgcct ccgagctcgg ctggtcagag
     1681 gagcgagtcg ctgcgatcga cgcggatggg aaccgcctgc tcgacctcgg ggagttggca
     1741 aatatcagcc agggcgcccc cgacatcgaa atgtcggtcg atctgcgtgc tctggatgca
     1801 gccggtggtc tgttgcacgt gctgtcaacc gtcggagagc ggctcgatga tgccacgcga
     1861 ctcgattatg cgaagatctc gtttgattcc gccgtggtga ccttctcgca ccgcaatctc
     1921 gatccggtcg ccgacgccat cgacgacgcg atgcagcaat tcaacctgct cgacgccgac
     1981 gccaatggct acctcagccg cgacgaagtg gccgagcgaa ttcgctttca gcgggagctg
     2041 ttcgacctga tggacggcga cggcgacgac aaagtatttg tcgatgaaat gaaggcctat
     2101 gtgaaagccc gtgccgagcc ggctgcaggc acttgccggg tgaatgttta tgatgccggc
     2161 aacggctact ttatggccct cgatacgaac gcggatggcc gcgtctcaga gcgcgagaag
     2221 cgcaaggcgg cccagtcgct cgccc
//
LOCUS       DASUGB010000953        10066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1912481, whole genome shotgun sequence.
ACCESSION   DASUGB010000953 DASUGB010000000
VERSION     DASUGB010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912481"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFV87_18035"
     CDS             complement(<1..1346)
                     /locus_tag="VFV87_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX5105728.1"
                     /translation="MAPTDYLRETHHEFLAASEQALADPGLQQILGQLGDTLGKRNRD
                     AWAALPNSDLIRERARAIKDQTLAELDKHLATLEQSVVARGGRVHWAEDGAAARRIVL
                     EIAERRKARLVVKSKSMTSEEIHLNAALEHAGIETVETDLGEYIIQLAGHRPSHIVAP
                     AIHLSAQQIADILSKPAGERLPVEREILAGFARQALRQKFAEADIGLSGANFAIAETG
                     SIVLVTNEGNGRLTCALPGCHIAIMGMEKVIPRLADLPYFLKVLARGATGQKLSVYTQ
                     IITGPRQAGEIEGPDEFHLVILDNGRSKILGSPLRESLFCIRCGACLNACPVYRSVGG
                     HAYGGVYAGPIGAVLTPLYDGLAPNHHLPHASSLCGACQAACPVKIQIPEMLIRLREQ
                     LHHTPEHRSRLEALAYGLWARSLRMPWLYRLSTWLATRTIGRFKRADPWLKHLPGKL"
     gene            complement(1365..1748)
                     /locus_tag="VFV87_18040"
     CDS             complement(1365..1748)
                     /locus_tag="VFV87_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105729.1"
                     /translation="MSARKTKGRNSKRTESRPIDAGESRSAVALTVVWMLTLMSTTVA
                     LVMTAIAWLVVLAFPVAAGRAHPLGFLPGLLVFVAATTGSLCLLLALLVHRVRQIPPP
                     LAITVAAALIGVAPLVTIAILAFLG"
     gene            complement(1837..2265)
                     /locus_tag="VFV87_18045"
     CDS             complement(1837..2265)
                     /locus_tag="VFV87_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105730.1"
                     /translation="MTCWSKLMTLAAIALVVALGAPQKAMASSAAVSAAAAGGGCCTP
                     TEICKDPRCISYKSHRFLRCKWDPCHTQKTVLQVPDYCCCCLVEVPVCIPACCSGAPT
                     VCSHCGHFGHDVVEYSWCCGYHLKVVFDKCGNVVVHSYGL"
     gene            2617..3603
                     /locus_tag="VFV87_18050"
     CDS             2617..3603
                     /locus_tag="VFV87_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105731.1"
                     /translation="MPTSVASNSLTDVVRPLEQTAAEFRHECQALDQMSETLFAEIEQ
                     LRQELEHRANALEDGRRRLAERGRQLAEQRKETGRLTQLLEQQDSHLTEALSQLKSLR
                     EQIDRERSEAHQREAEHVAALEKRLQESQSQREELNHQLQVVQATAASGGGGGESLAP
                     LLAELGDLRRQISETHGELAETRTQLSEAIERSAAARASSSEGGPSVGASGDFSALER
                     ERTELESELELVRTRATELQETVNTQRRELAEQRADFASELRMLRELIEQNQSDGSFS
                     RPREESLTLVGAPNGGRIQGESEDAPPDPVISSVMAQFARLQKDVAQRRKKK"
     gene            3655..4875
                     /locus_tag="VFV87_18055"
     CDS             3655..4875
                     /locus_tag="VFV87_18055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5105732.1"
                     /translation="MRLSLKNLNFNWLCLGAVPVCLLLGWATGYWMLGAGGAAAALCV
                     LAIRQPQFPWRLVRQSARPKPVEEPESPLPPRKAVRRGGDGSLVDRMVASNRVPLLLR
                     PQIASSLGPSDLAAAQAALDETMAIVPQGPVAVRARCYESLDEEHAPRAERLIQVEGF
                     FLDRFTVTNQEFQQFVEEGGYEQMALWDESIWPAVLGFVDRTGHPGPRYWENGCYPRG
                     TEDHPVVGVSWYEASAYSRWTGKRLPTDPEWVKAGGWPVMSECGRPVQRKFPWGDAMD
                     RRRANMWGSGWDGTVAVHALPEGVSVGGVQQLIGNVWEWTSSTFGAWEPSNRKIETHL
                     PLKSIRGGAFDTYFDTQANCQFQSGESPLSRKHNVGFRCALGFCDVVHMADSVDADAT
                     DELPGIPAGEEVHA"
     gene            4872..5777
                     /locus_tag="VFV87_18060"
     CDS             4872..5777
                     /locus_tag="VFV87_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105733.1"
                     /translation="MRAIGNLELDSYRLAAYTVPCPCYICGGGNNFDAELCRHCHAPM
                     ALAHQATTQKVHPKMVAAIGPSGAGKTVYMGMLADLLSRQDENMQLLARGAFSIKLQQ
                     SAMASLARCEFPQKTPNEPDRWNWVHCQVLRKKHRPAELIMPDLAGEALLEEVDHPNT
                     FPVIRAFLQKCAGVLVLIDAPRAECGESDEDFHTMKLISYLCELQSDKKTGWQTRPVA
                     LVFTKADQCEHCFDDPQAFARRHTPGLLQQCRERLKKHEFFAVGVAGACGFRNEPHGK
                     VQVPLRIEPRGIVEPFKWLLEQFGT"
     gene            5864..6634
                     /locus_tag="VFV87_18065"
     CDS             5864..6634
                     /locus_tag="VFV87_18065"
                     /inference="COORDINATES: protein motif:HMM:NF040416.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105734.1"
                     /translation="MRLEQAIFTSLRSQRLDGYQLAAHSSGISEEIAKELATWGPAHD
                     SLWSHERDAASVNFHRLAGGLFCISQTSLAGAEYSGRGGGRVYTQMLVVPREALARFA
                     DDPFQILQAVAASGRLLVHEQVPDSLPTIPLLGRGRELGRDAMQPLIDVIGAETLAEL
                     TEVATSNWAATVITNVPVPRLFQVLLHQLAPDERLEVSFTTGLRPSSRRPFKLSIVPD
                     DPALARQSQRMSGGRIIEARADDPTILKFSPPRTPDVV"
     gene            complement(6678..7892)
                     /gene="carA"
                     /locus_tag="VFV87_18070"
     CDS             complement(6678..7892)
                     /gene="carA"
                     /locus_tag="VFV87_18070"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5105735.1"
                     /translation="MTTDKPAKLALEDGTVYSGTSFGAEGEVDGEVCFNTSMTGYQEI
                     LTDPSYRGQIVTMTYTEMGNYGVNPEDVESAKPHLAGFIVKEHAERPSNFRSQESLAA
                     YLKRYGIPGLAGIDTRALVRRLRTRGAMKGVLSSVDLDDASLVAKAKASPGLVGRDLV
                     REVVPTQARQWDAGLSKWAKLPAEANPAFPAEEFHVVALDYGMKWNIARHLKDEGCRI
                     TILPGTATAEEVLSHQPDGVFLSNGPGDPEPLVYAQDCIRGVLGKVPVFGICLGHQLL
                     GLACGAKTFKLKFGHRGANQPVLNVDAGKVEITAQNHGFAVDEDSMPSCLEVTHRNLN
                     DNTVAGLRHKEVPAFSVQYHPESSAGPHDSRYLFRQFRQAMEAERAGAYARGQRAGDG
                     RRQRARLLNGEL"
     gene            8415..>10066
                     /locus_tag="VFV87_18075"
     CDS             8415..>10066
                     /locus_tag="VFV87_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105736.1"
                     /translation="MSLDAYQPCPCGIDKKIKFCCGAEIIDDLGKIEAALDGDQRLGA
                     LDLCNKLLAQKPDRPCLLMHKSMVQMALRELGPCRETVDHLLEVAPGNPAGLGMGAML
                     DCQEGKPDEAVLKLQQALEAQQGMLIPTVYQAIGIVARTLSVIGEPLAAQAHAAFQAA
                     ASQGQDRNAVMTLLEVESGGQIPLAVQGINHLVRIDSDCKLTAAGIAAFNEALKQADL
                     GCWLAGAKKFEALAAREPNEPAVWRNIGVLRTRVLDHPAAIAALRKYASFASVPRDDA
                     VESEALAQYLSEPAEVDTLPELTVTFSVTDISALQEHLLSSKRVQSVPYNPEQFREEN
                     QPPPMSAFLLFDREIPATSQNLTRDNVPKVLGELWLYGKETDRLARVEFTSIKTADFA
                     AKLKHLEEALGPHAGKQEKEEETGRIAAAAAALAINWRFPDDTPVELRKKLIEEHRTQ
                     TLLSIWPNLPMGVLDGKSPRQVVGDPAGAIRVQAIILQMDLAEPVENPDLNKLRRSLG
                     LPTIEPLDPSGVRVDTLSPARQTRLMVEKLTDEQLVAVYRRAA"
BASE COUNT         1877 a         3240 c         3205 g         1744 t
ORIGIN      
        1 agcttgccgg gcaggtgctt gagccacgga tccgcacgct tgaagcggcc gatcgtccgc
       61 gttgccagcc aggtggacag gcgatagagc caaggcatac gcagcgagcg ggcccagagg
      121 ccataggcca gtgcctccag acggctcctg tgctcgggcg tgtgatgaag ctgctcgcgc
      181 aggcggatca gcatctccgg aatctgaatc ttcacggggc acgccgcctg gcacgcgccg
      241 cacaggctcg acgcgtgcgg cagatggtgg ttcggcgcca ggccgtcata gagcggcgtc
      301 aggaccgcgc cgatcgggcc ggcatagacg ccgccatacg cgtgtccgcc gacgctgcga
      361 tagacggggc aagcgttcag gcacgcccca cagcgaatgc agaacaggct ctcccgcagc
      421 gggctgccga gaatcttcga gcggccgttg tcgagaatca ccagatggaa ctcgtccggc
      481 ccttcgatct cacccgcttg tcgcggcccc gtgatgatct gcgtatagac cgacaacttc
      541 tgccccgtcg cgccgcgggc gagcaccttc aggaaatacg gcagatcggc cagccgcgga
      601 atgaccttct ccatccccat gatcgcgatg tggcacccag gcaacgcaca cgtcagccgg
      661 ccgttgcctt cgttggtcac cagcacgatc gagccggtct cggcgatggc gaaattcgcc
      721 ccgctgaggc cgatgtcggc ttcggcgaac ttctgccgca gggcctgtcg cgcgaagccg
      781 gccaggattt cgcgttccac gggcagccgc tcgcccgcgg gcttgctgag gatgtcggcg
      841 atctgctgag ccgaaaggtg aatcgccggg gcgacgatgt gcgacgggcg atgcccggca
      901 agttggatga tgtactcgcc caggtcggtt tcaaccgttt caatgccggc gtgctccagg
      961 gccgcgttca gatggatttc ctcgctcgtc atcgacttcg acttgaccac cagccgggct
     1021 ttccgccgtt cggcgatttc cagcacgatg cggcgggccg ccgcgccatc ttcagcccaa
     1081 tggacgcggc cgccgcgggc gacgacgctc tgctccagcg tcgccaggtg cttgtcgagc
     1141 tcggcaagcg tctggtcctt gatcgcgcgg gcccgctcgc ggatcaggtc ggaattcggg
     1201 agcgcggccc aggcgtcgcg gttgcgcttg cccagcgtat cgcccaactg gccgagaatc
     1261 tgctgcaggc cgggatcggc gagcgcttgc tccgacgcgg cgagaaactc gtgatgggtt
     1321 tcgcgaaggt agtcggtagg agccacgaag gtgcgccgcg gtgctcagcc caggaatgcc
     1381 aggatggcga ttgtaacgag cggagcgacg ccgatcagcg ccgcagcgac ggtgatggcc
     1441 aaggggggcg gaatctggcg cacgcgatgg accagcagcg ccaagagcag gcagaggctg
     1501 cccgtcgtcg ccgcgacgaa gaccagcaac ccgggcagaa agccgagcgg atgtgcccta
     1561 cctgcagcca cggggaaggc gagcaccacg agccaggcga tggccgtcat gaccagggcc
     1621 accgtcgtgc tcatgagcgt gagcatccaa acgacggtga gggcaacggc cgagcggctt
     1681 tcgcctgcgt cgatgggccg tgattcggtg cgtttcgagt ttcggccttt ggtcttgcgg
     1741 gcggacaatt ggagtcggct gtctcgtggg gtggaaatgc aacggggcgg caaccttgcc
     1801 accccgctca ttctagcccg gcgtcaacct gttcgttcag aggccgtagg aatgcacgac
     1861 cacattgccg cacttgtcga agaccacctt gaggtgatag ccgcagcacc aggagtattc
     1921 gaccacgtcg tgtccaaagt ggccgcagtg cgagcacacc gtcggcgcgc cgctgcagca
     1981 ggcaggaatg caaacgggga cttccacgag acagcagcag caatagtccg gaacctggag
     2041 gacggtcttc tgcgtgtgac aaggatccca cttacaccgg aggaagcggt ggctcttgta
     2101 cgaaatgcag cgcgggtctt tacagatttc ggtcggcgtg cagcagccac cgccggcagc
     2161 agcggcactc acggcggcgc tgctggccat ggccttctgc ggcgccccta gggcgacaac
     2221 caaagcgatt gccgcaagcg tcatcaattt cgaccagcaa gtcataaggg aatctccaag
     2281 ttgggaaggc tggcggattt ggaacgcatc aacagccata aacatgacac atcttctcca
     2341 atccgcaagg gcgctacaga gcccccattt tatgtcggcg caacctattt gcggtcaaga
     2401 aattatgcga tccgactacg ctatcccccg caaagcggca cgcctgctcc gcctgtttgc
     2461 caatgcccca cgggtgacct aggttttcgc tggcggaccg cctcatttgg tcacgttccg
     2521 ccgaccgcgc ggtactaccg gcatgttcat tgtttggagc cggctgaccg tggggcgacc
     2581 cgtggagggt tggactcgct agcgaaaggc cattccgtgc ccacttcggt tgcatcgaac
     2641 tcgctcacgg acgtcgtccg tccgctggaa cagaccgcag cggagttccg gcatgagtgt
     2701 caggcactcg accagatgag cgagacgctc tttgcggaaa tcgagcaact gcggcaggag
     2761 ctcgagcacc gagcgaacgc cctggaagac ggccgccgcc ggttggcgga acgcgggcgc
     2821 cagctcgccg aacaacgcaa ggaaacaggc cggctgacgc aattgctaga gcaacaggat
     2881 tcgcacctga ccgaagcgct ctcgcagctc aagtccttgc gggaacaaat cgaccgcgaa
     2941 cgcagcgaag cccaccagcg ggaagcggaa cacgtcgcgg cgctcgagaa gcgcctgcag
     3001 gaatcccagt cgcagcgcga ggaattgaat catcagctac aggttgtgca ggccaccgct
     3061 gcatcgggcg gcggcggggg cgaatctttg gccccgctcc tggcggagtt gggcgatctc
     3121 cgccgccaga tttccgaaac acacggcgag ctcgccgaaa cgcggactca actgagcgag
     3181 gcgatcgagc gctccgctgc cgcccgcgcg tcgtccagtg aaggcggacc atccgttggc
     3241 gcttccggcg acttctccgc cctagagcgc gaacggaccg agttggaatc cgagctggaa
     3301 cttgtccgga cccgcgcgac agagctgcag gaaacggtca atactcagcg ccgcgaactg
     3361 gccgaacagc gggcggattt cgcctccgag ctccgcatgc tgcgggagct gatcgaacag
     3421 aaccagagcg acggcagctt cagccggccg cgcgaagaat cgctgacgct ggtcggggcg
     3481 cccaatggcg gccgcattca gggcgagtcg gaagacgccc cacccgatcc cgtcatcagc
     3541 tcggtcatgg cgcagttcgc ccggctgcag aaggacgtgg cccagcggcg gaagaaaaaa
     3601 taactccaca cctgcggaac gttctgcggg atcgaccctc gcgaggtaat cgtcatgaga
     3661 ctgagcctca agaatctgaa cttcaattgg ttgtgcctgg gcgccgtccc ggtttgcctg
     3721 ttgctcggtt gggcgacggg ctattggatg ctcggcgccg gcggagcagc tgccgcgctc
     3781 tgtgtgcttg ccattcgcca accgcaattc ccctggcgtc tcgtgcggca atcggctcga
     3841 ccgaagcccg tcgaggagcc cgaatcacca ctgccgccca ggaaggcggt ccggcgcggc
     3901 ggcgatgggt cgctcgtcga tcgcatggtc gccagcaacc gtgtgccgct actgcttcga
     3961 ccgcagatcg cgtcgagcct cggccccagc gacctggccg ccgcccaggc ggcgctggac
     4021 gaaacgatgg cgatcgttcc ccaaggtcca gtcgccgtgc gtgctcgatg ctacgaatcg
     4081 ctcgatgaag aacacgcgcc acgggcggaa cgactcatcc aggttgaggg gttctttctc
     4141 gaccgcttca ccgtgaccaa tcaagagttt cagcaattcg tcgaggaagg cggctacgag
     4201 caaatggccc tctgggacga atcgatctgg ccggcagtct tgggctttgt cgatcgaacg
     4261 ggacatcccg gaccgcgata ctgggagaac ggctgctatc cgcggggaac ggaagaccat
     4321 cccgtggtcg gcgtctcctg gtacgaagcc agcgcctact cccgttggac cggcaagcgt
     4381 cttccgaccg atccggaatg ggtcaaggcc ggcggctggc cggtgatgtc cgaatgcgga
     4441 cggcccgtgc agcgaaagtt tccctggggc gatgccatgg accgccgccg ggccaacatg
     4501 tgggggagcg gctgggatgg aaccgtcgcg gtccacgccc tgcccgaggg ggtcagcgtc
     4561 ggcggcgtgc agcagctcat cggcaacgtc tgggaatgga ccagcagcac cttcggcgcc
     4621 tgggaaccat cgaatcgcaa gatcgagaca cacctgcccc tgaagagcat ccgcggcgga
     4681 gcgttcgaca cgtatttcga cacgcaggcc aactgccaat tccaaagcgg agagagtccg
     4741 ctgtcgagga aacacaacgt cggctttcgc tgtgccctgg gcttttgcga cgtggtccat
     4801 atggccgaca gcgtggatgc cgacgcgacc gacgagttgc ccggcattcc cgcgggtgag
     4861 gaggttcacg catgagagcg atcggaaacc tggagctcga ctcctatcgg ctggcggcct
     4921 atacggtccc ctgcccgtgc tacatctgcg gcggcgggaa caacttcgac gctgagctgt
     4981 gccgccattg ccatgcgcca atggccttgg cccatcaagc gacgactcaa aaagtccatc
     5041 ccaagatggt cgccgccatc ggcccgtcgg gagcgggcaa gacggtctat atgggcatgc
     5101 tcgccgacct gctttcgcgg caagacgaga acatgcagct tctggcccgc ggggccttct
     5161 cgatcaagtt gcagcagtcc gcgatggcct cgctcgcccg ctgcgagttt cctcagaaga
     5221 cgcccaacga gcccgatcgc tggaattggg tccattgtca ggtgctccgc aagaagcatc
     5281 gacccgccga attgatcatg cccgacctgg ccggcgaagc gctcctggag gaagtcgatc
     5341 atccaaacac ctttcccgta atccgcgcct tcctgcagaa gtgcgccggc gtgctggtcc
     5401 tcatcgacgc tccccgcgcc gaatgcggcg aaagcgatga agacttccac acgatgaaac
     5461 tcatcagcta cctctgcgag ctgcaatccg acaagaaaac cggctggcaa acccggccgg
     5521 tggccctcgt ctttacgaag gccgatcaat gcgaacattg cttcgacgat ccgcaggcct
     5581 tcgctcgccg ccacactccc ggtttgctcc aacagtgccg cgagcgactg aaaaagcacg
     5641 agttcttcgc cgtcggcgta gcaggagcgt gcggcttccg caacgagccg catggcaaag
     5701 tccaagtgcc gttgcggatc gagccgcggg gaatcgtcga gccgttcaag tggctgctgg
     5761 aacagttcgg gacgtagcaa cacgaacccg aagcgtcagc gagggtctag cctcctgttc
     5821 gcgatcggtg actccctcgc tgacgcgtcg ggttaatatc gccatgcgat tggaacaagc
     5881 catcttcacg tcgctccgca gtcaacggct cgacggttac caactcgccg cccacagctc
     5941 gggaatcagc gaggagattg ccaaagaact ggcgacgtgg ggaccggctc acgattcgct
     6001 ttggagccat gagcgcgacg cggcaagcgt gaactttcat cggctcgccg gcggtctgtt
     6061 ctgcatctcc cagacgtcgc tggccggtgc tgaatacagc ggccgcggcg gcggccgcgt
     6121 ctatacccag atgctcgtcg tgccgcgcga ggcgctcgcg cgcttcgccg acgatccatt
     6181 tcagatcctg caagccgtag ccgcttccgg aaggctgctt gttcacgaac aggttcccga
     6241 ttctctgccg acgatcccgc ttctgggccg gggccgtgaa ttgggccgtg atgccatgca
     6301 gccgctgatc gacgtcatcg gcgccgaaac actggccgag ctgaccgaag tggctacgtc
     6361 gaactgggcc gccaccgtca tcacgaacgt gccggtccca cgccttttcc aagttctcct
     6421 gcaccaactc gcacccgatg agcgcctgga ggtttcgttc accaccgggc tgcggccttc
     6481 ctcccgccgc cccttcaagc tctcgatcgt ccccgacgac cccgccctcg cccggcaatc
     6541 ccagcgcatg agcggcggcc ggatcatcga agcccgggcg gacgacccaa cgatcctcaa
     6601 attctcaccg ccgcgcacgc ctgatgtcgt gtagcgcgaa gccgaaggcg ccgcgccgga
     6661 gtagcgatcg acatcgacta cagctcgccg ttcaataacc tcgccctctg tcgtcgcccg
     6721 tctcctgccc tctgtcctct tgcgtacgct cccgcccgct ccgcttccat cgcctgtcgg
     6781 aactgccgga agaggtaacg gctgtcgtgc gggccggcgg aggattcggg atgatattgg
     6841 acgctgaagg cggggacctc cttgtgccgt aggccggcga cggtgttgtc gttcaaattg
     6901 cggtgtgtca cttccaggca ggatggcatc gaatcctcat cgaccgcaaa tccgtgattc
     6961 tgggcggtaa tctcgacctt tcccgcatcg acgttcagta ccggctggtt ggcgccgcgg
     7021 tggccgaact tgagcttgaa ggtcttcgcc ccgcaggcca ggcccagcag ttgatgcccg
     7081 aggcaaatgc caaacacggg cactttgccg aggaccccgc ggatgcaatc ctgggcatag
     7141 accagcggct ccgggtcgcc ggggccgttg ctcaggaaaa cgccgtccgg ctggtgcgat
     7201 agcacttcct ccgccgtcgc tgtgcccggc aggatcgtga tccggcagcc ttcgtccttc
     7261 aggtgccggg cgatgttcca cttcatgcca tagtcgagag cgacgacgtg gaattcttcg
     7321 gcagggaacg ccgggttcgc ctcagccggc aactttgccc actttgacag gccggcatcc
     7381 cactggcgtg cttgggtggg cacgacctcc cgcaccagat cgcggccaac gagtcccggg
     7441 ctggccttgg ccttggcgac caggctggca tcatccagat cgacgctcga cagcacccct
     7501 ttcatcgccc cccgcgtccg cagccggcga acgagggccc gcgtatcgat gccggcgagc
     7561 ccagggattc cgtaccgctt caggtacgca gcgagcgact cctgcgagcg gaaattgctc
     7621 ggtcgctcgg catgctcctt gacgataaaa ccggccaggt ggggcttggc gctttcgaca
     7681 tcctcgggat tgacaccgta attgcccatc tcggtgtagg tcatcgtgac gatctgcccg
     7741 cgatagctcg gatcggtcag gatctcctga tagccggtca tcgaggtgtt gaagcagact
     7801 tcgccatcca cctcgccttc cgcgccgaat gaggtgccgg aatagacggt gccgtcttcg
     7861 agggcaagct ttgcgggttt gtcagttgtc attggtcagt cgtgggacta agcgatgctt
     7921 cgtccttgcg ggattggttc gtggatgaag cccggcttcg tccgatgcgc cctggcaatc
     7981 tgtggcggat tctccggcgc gatagcacgc cctcaaggcc gcccaggcgg gcttccgata
     8041 cccaactaac ggctgtgggt cttcggactt tgggcaacca gaatccgaaa cagccaaact
     8101 tgggaggcct cttgttgcgg cgagcaagag acctcctttt tttgttgcgc cgcaagcgaa
     8161 gctactactg tcctatccgc caaacccgaa acgtctcgga ggccgtccct acatgcgaag
     8221 cgctcatgcg cattgtatcg ttcctaccaa tcgagggaac gacctcgatt ccgttggcag
     8281 ggcgctgcca cctaggtggg cctgcgaacg accggcgcta cgatgctgcc gattgcaagc
     8341 gcgaagcctc ctaccagccg gctgcgtgga aacttagact ctagggttcc acttaccccc
     8401 gaaaggcggt tggcatgtcg ctggatgctt atcagccctg cccgtgcggc atcgataaga
     8461 agatcaagtt ctgctgcggc gccgagatca ttgacgacct gggcaaaatt gaagcggccc
     8521 tcgatggcga tcagcgattg ggcgcgctcg atctgtgcaa caagctcttg gcccagaagc
     8581 cggaccggcc gtgcctcctg atgcacaagt cgatggtgca gatggctttg cgcgagctgg
     8641 ggccgtgccg cgagaccgtc gatcacctgc tggaggtggc gccgggcaat cccgccgggc
     8701 tgggcatggg ggcaatgctc gactgccagg aaggcaagcc ggacgaggcg gtcctcaagc
     8761 tgcagcaggc cctggaagcc cagcagggga tgctcattcc gacggtctat caggcgatcg
     8821 gcatcgtcgc ccgcacactt tccgtgatcg gcgagccgct ggcggcgcag gcccatgccg
     8881 catttcaggc tgctgccagc caaggccagg accggaatgc ggtgatgacg ctcctcgagg
     8941 tggaatcggg cgggcagatt ccactcgccg tgcagggcat caatcacctc gtgcggatcg
     9001 attccgactg caagctcaca gccgcgggca ttgccgcatt caatgaagcc ctgaagcagg
     9061 cggacctggg ctgctggctg gccggggcga agaagttcga ggctctcgcc gcccgcgagc
     9121 cgaacgagcc cgcggtctgg cgaaatattg gagtgctgcg gacgcgggtg ctcgatcatc
     9181 cggctgcgat cgcagccctg cgaaagtatg cctcgttcgc ctcggtgccg cgcgatgacg
     9241 ccgtcgaatc cgaggcgctg gcccaatatc tcagcgagcc agccgaagtc gatacgctgc
     9301 cagagctgac ggtcactttt agtgtgacgg acatttcggc gctgcaggag cacctgctct
     9361 ccagcaagcg cgtgcagtcg gtgccataca accccgagca gttccgcgag gaaaaccaac
     9421 cgccgcccat gtcggccttc ctgctgttcg atcgggagat tccggccacg tcgcaaaacc
     9481 tcacgcgcga caacgtgccg aaagtgctgg gcgaactgtg gctatatggc aaggagaccg
     9541 accgactggc acgcgtcgaa ttcacatcga tcaagacagc cgatttcgcc gccaagctca
     9601 agcacctgga agaggccctc ggcccgcacg cggggaagca ggagaaggaa gaagaaaccg
     9661 gccgcatcgc ggccgcggct gcggcgctgg cgatcaactg gcgattcccg gacgatacgc
     9721 ccgtggagct gcgcaagaag ctcatcgaag agcatcgcac gcaaacgctg ctcagcatct
     9781 ggcccaatct gccgatgggc gtgctcgacg gcaaaagccc gcggcaggtg gtcggagatc
     9841 cggctggggc gattcgcgtc caggcgatca tcctgcagat ggatctggcc gagccggtgg
     9901 agaacccaga cttgaacaag ctccgtcgct cgctcggcct gccgacgatt gagccgctcg
     9961 atccttcggg cgtgcgcgtc gatacgctgt ccccggcgag gcaaacgcgg ctgatggtgg
    10021 agaagctgac cgacgagcag ctggttgccg tttatcggcg ggcggc
//
LOCUS       DASUGB010000954         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_662501, whole genome shotgun sequence.
ACCESSION   DASUGB010000954 DASUGB010000000
VERSION     DASUGB010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662501"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VFV87_18080"
     CDS             <1..1416
                     /locus_tag="VFV87_18080"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5105737.1"
                     /translation="VGRRNQPSVAVLQFEKALDFPAGTQLKVAWRQTDMLGCCRWSIT
                     RKASPSAPPIDHGAVLAVQTPPAERSTEQQAAIFAAWRSSLADAKATSDEIAAIWKEY
                     PQAATSILHLIEREPKNHRDTHRLDRGNWDQPMQVIEPHTPAAFHPFPADAPRNRLGL
                     AKWLTDKRSPLTARVAVNRAWQAMFGAGLVETPEDFGTRAPIPEYRELLDWLAVDFME
                     HGWSQKHLIRTIVTSGVYRQTSVTSPKLHERDPRNVWLARGPRFRAEAEVVRDIALSV
                     SGLITQKLGGPSVIPPVPQNVLDYNFTYPTFWKPAEGPERYRRTLYGFRKRSMPDPVM
                     ANFDGPNGDFACARRARSNTPLAALTGLNETIFVESARAFALRVLREGGGSDADRADY
                     AFFLCTSRKPTADERQAILDLLASRRKRLAEGWLNPREIATGDPAKLPALPGDATPQD
                     AAAWTLAARVLLNLDETISKN"
     gene            1521..>2564
                     /locus_tag="VFV87_18085"
     CDS             1521..>2564
                     /locus_tag="VFV87_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105738.1"
                     /translation="MNLSHQLHSARAQYLSRRWFLRDCGIGLAGITASSLLSAEAAAN
                     APATDNPLAPRAPHFAGKAKRVIYIFQAGAPSHLELFDNKPELTKRNGQLPPAELLKG
                     YRAAFINPNSALLGPKFKFEKHGKCGMELSEVLPHTAKIADELCLIRTMQTEAVNHAP
                     AQIMMNSGSQQFGRPSFGSWTLYGLGSESANLPGFVVLTSAKGTSGGASNYGNGFLPT
                     IYGGIPFRSSGDPVLYLSNPAGVDADTQRASLETLNKLNDLSLKAAGDPEIAARIQSY
                     EMAYRLQTSAPELMDLSQEKQQTLDRYGIKSPKDANFARNCLLARRLIERGVRFVQVW
                     HGQGQPWDNHDDIE"
BASE COUNT          485 a          877 c          771 g          431 t
ORIGIN      
        1 gttggccgcc gcaatcagcc ctcggtcgcc gtgctgcaat tcgagaaagc gctcgatttt
       61 cccgccggga cgcagctcaa agtcgcctgg cgtcaaaccg atatgctcgg ttgttgccgc
      121 tggagcatca cccgcaaggc atctccttcc gcgccgccca tcgatcacgg ggccgttctg
      181 gcggtgcaaa cgccgcctgc cgagcggtcc acggagcagc aagcggccat tttcgcggcc
      241 tggcgaagca gcctggccga cgcaaaagcg acgagcgacg aaatcgcggc gatctggaag
      301 gaatacccgc aggccgccac ctccatcctg catcttatcg agcgggaacc gaagaatcac
      361 cgcgacacgc accgcctcga ccgaggcaac tgggatcagc ccatgcaggt gatcgagccc
      421 catacgccgg ccgcgtttca tccgttcccg gcggacgcac cgcggaaccg tttgggcctg
      481 gcgaaatggc tgaccgacaa gcgttccccc ctgacggctc gcgtcgccgt caaccgcgcc
      541 tggcaggcga tgttcggcgc aggcctcgtc gagacgccag aggactttgg gacgcgagcg
      601 ccgattcccg agtatcgcga gctgctggac tggctcgccg ttgatttcat ggagcacggc
      661 tggagccaga agcatttgat tcggacgatc gtgaccagcg gcgtctatcg ccagacttcc
      721 gtcacgtcgc cgaagctgca cgagcgggac ccgcgcaacg tctggctggc tcgcgggccg
      781 cggtttcgcg ccgaagccga ggtcgtgcgc gacatcgccc tctcggtttc cggcctgatc
      841 acgcagaagc tcggcgggcc gagcgtcatt ccgcccgtgc cacaaaacgt cctggactac
      901 aacttcacgt atccaacatt ctggaagccg gcagaggggc cggagcgcta tcgccgcacc
      961 ctgtacggct ttcggaagcg ttcgatgccc gatccggtga tggcgaattt cgacgggccc
     1021 aacggtgatt ttgcttgtgc ccgccgcgcc cggtccaata cgccgctggc cgccctcacc
     1081 gggctcaacg agacgatctt tgtggaatcg gcgcgggcct tcgcgctccg cgtcctccgc
     1141 gagggaggcg gcagcgatgc cgaccgggcc gactatgcct ttttcctgtg cacgtcacgc
     1201 aagccgacgg cggacgagcg gcaggcgatt ttggatctgc tggcgtcgcg ccgaaaacgg
     1261 ttggcggaag ggtggctcaa tccgcgggag attgcgacgg gggacccggc caaactgccg
     1321 gcgctccccg gcgatgccac tccccaggat gcagccgcct ggaccctggc agcccgcgtg
     1381 ctgctgaacc tcgacgagac catttccaag aactagcacg aacccacaca ctaacccgaa
     1441 gcgccagcga gggagatgag ccccactggc ccgccgccct cgctcgcgct tcgggttagt
     1501 ggaaagaatc gagacctgct atgaatctct cccaccaact ccactccgcc cgcgcccaat
     1561 atctcagccg ccgctggttc ctgcgggatt gcggcattgg cctggccggc attaccgcca
     1621 gttcgctcct ttccgccgaa gccgccgcaa atgcaccggc gaccgacaat ccgcttgctc
     1681 ctcgcgcacc ccattttgcc ggcaaggcca agcgggtgat ttacatcttc caggccggcg
     1741 cgccgagcca cctggagctg ttcgacaaca agccggaact taccaagcgc aatgggcagt
     1801 tgccgccggc cgagctgctc aagggttatc gggcggcctt catcaatccg aactccgctc
     1861 tgcttggccc gaaattcaag ttcgaaaagc atggcaagtg cgggatggag ctgagcgaag
     1921 tcctgccgca taccgccaaa atcgcggacg agctctgcct catccggacg atgcaaaccg
     1981 aagcggtgaa tcacgcgcca gctcagatca tgatgaacag cggctcgcaa cagttcggcc
     2041 ggcccagttt tggatcctgg acgctgtacg gcctcggcag cgagtcggca aatttgcccg
     2101 gttttgtcgt tctcacgagc gctaagggaa cgagcggcgg ggcgagcaac tacggcaacg
     2161 gattcctgcc gaccatttac ggcggcattc ccttccgcag ctcgggcgat cccgtcttgt
     2221 atctctccaa tccggcggga gtcgatgccg acacgcagcg ggcgtccctc gaaacattga
     2281 acaagctcaa cgacctttcg ctgaaagctg ccggcgatcc ggaaatcgcg gcccgcatcc
     2341 agagttacga gatggcctat cgcctgcaaa ccagcgcccc cgagctgatg gatctctcgc
     2401 aggaaaagca gcagacgctc gaccgttacg gcatcaaatc gcccaaggac gcgaacttcg
     2461 cccgcaattg cctgctggcc cgccgcctca tcgagcgcgg cgtccgcttt gtccaagtct
     2521 ggcatggcca gggccagccc tgggacaacc acgacgacat cgag
//
LOCUS       DASUGB010000955         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1495656, whole genome shotgun sequence.
ACCESSION   DASUGB010000955 DASUGB010000000
VERSION     DASUGB010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495656"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..731
                     /locus_tag="VFV87_18090"
     CDS             252..731
                     /locus_tag="VFV87_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5105739.1"
                     /translation="MTVTTTTGTVTVNAAFLQEIKEVNQELWQLLRDLRHRCQRPVAP
                     GACRLLIDKLSTLRDQLALHFSLEEAYGYFDEPLDVAPQLCRAADQLRAEHKTLYIDF
                     CDLVDHAERMFYEEQHAALALWISPQFLALDLRLREHEERENELIYEAYDDDIGVGD"
     gene            complement(728..1033)
                     /locus_tag="VFV87_18095"
     CDS             complement(728..1033)
                     /locus_tag="VFV87_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5105740.1"
                     /translation="MPQWVRVAAASDLAPGTCGEYVAGERIVALFNVDGQFYALDGIC
                     PHQGGPLGKGRLAGCIVTCPWHGFQFDVRTGQHQTSKSLVHPTFPVKVEGGGVFVEV"
     gene            complement(1212..2174)
                     /locus_tag="VFV87_18100"
     CDS             complement(1212..2174)
                     /locus_tag="VFV87_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5105741.1"
                     /translation="MRRLRHLAEYLAVRVSLSLIQAVSLKTCQAYIRIVAWLAADVLK
                     IRRRVVDENLRISFPGLTEGERKRLARAMWEHLLLMVCEVAHVQRKLHDTNWREHVTD
                     YNSRELLRLAFSRRPTVAVAGHFGNFEICGFMAGFWGVRVYTVARPLDNPYLDRLVLR
                     FRQSMGHRILPTSDSANQADEVLQTGGMLVLLGDQHAPGRIGCVVNFMGRPASCHKAL
                     ALFSLLNRAPMLVVNCTRSGGPMQLRMGLDASIDPLEDRPETAGVKELTQWYNDILEA
                     QIRRQPEQYWWLHNRWKEIPASRPRKRKPQAAAAPSDQVQRPAA"
     gene            2336..>2682
                     /locus_tag="VFV87_18105"
     CDS             2336..>2682
                     /locus_tag="VFV87_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5105742.1"
                     /translation="MPSPSRSVLIVDPSAENREVLRTALERRGLRIFEADQADQGLQM
                     AREHHPHVIVLDLESDEPHASDVEDQYGAEARELHTSLVILGKACRRQRLDAARHVAK
                     PYHFAPLVHTIEQL"
BASE COUNT          476 a          909 c          826 g          471 t
ORIGIN      
        1 tccacaaaaa aagtgtttga ctcgcgccgg ggcaagcctt agcatggcgc gctgcggcgt
       61 gaagcacagc atgaggttgg ttcctttccc cggcttcccc ccatttctgg ggcgagcggg
      121 attcgcgtgt cctggcgctc agtccgcgcc gcgggacaag gggcattcgc cctccgcgga
      181 ctcaggctga gtgccccaaa aaaactcgca agggctttac ttatctgccc gctgcgagca
      241 cggaggtcgt catgactgtg actaccacga cgggaacggt gaccgtcaat gccgcgttcc
      301 tgcaggaaat caaagaggtc aaccaggagc tctggcagct cctcagggat ttgcggcacc
      361 gctgccagcg accggtcgcc cccggcgcct gccgcctgtt gattgacaaa ctcagcaccc
      421 tgcgcgacca gctcgcgctg cacttctccc tggaagaggc ttacggctat tttgacgagc
      481 cgctggatgt cgccccgcag ctgtgccgcg ccgccgatca gcttcgcgcc gagcacaaga
      541 ccctgtacat cgatttttgc gatctggtcg atcatgccga gcgaatgttc tacgaagagc
      601 agcacgcggc cttggccctc tggatcagcc cgcagttcct ggcccttgac ctgcgcctgc
      661 gcgagcacga ggagcgcgag aacgagctga tctacgaggc gtacgacgac gatatcggcg
      721 tcggagatta gacctccaca aacaccccgc cgccttcgac cttcaccggg aacgtcggat
      781 gcaccagcga cttgctcgtc tggtgctggc cggtacgcac gtcgaactgg aagccgtgcc
      841 agggacaggt gacgatgcag ccggcgagcc tccccttgcc gagcggccca ccctgatgcg
      901 ggcagatgcc gtccagggca tagaattggc catctacgtt gaatagcgcg acgatccgtt
      961 cgcccgcgac gtactcgccg caggttcccg gcgctagatc actcgcagcg gcgacgcgga
     1021 cccattgagg catgtcacac gctcctggga acaatcggag gcgactcgct ccgcgagtcg
     1081 cgaaggacgt gtttggctcg cggacgctgc gccccagcgg cgctgcagtc cacgagtccg
     1141 ctctaaggag cgcacagccg gttcgcttgc aatgtcgttc gcttctcgcg gagcgagaag
     1201 cctacggtgg cttacgccgc cggccgctgg acttgatcgc tcggcgcggc ggcggcttgc
     1261 ggcttgcgct ttctgggccg gctggcgggg atttctttcc agcggttgtg cagccaccag
     1321 tactgctcgg gctggcggcg gatttgcgct tccaggatgt cattgtacca ctgcgtcagc
     1381 tccttgacgc ccgccgtttc gggccggtcc tcgagcggat cgatcgacgc gtcgaggccc
     1441 atccgcaact gcatcggccc gccgctccgc gtgcagttaa ccacgagcat cggcgcccgg
     1501 ttgagcagcg aaaacaaggc cagtgccttg tggcacgaag cgggccgccc catgaagttc
     1561 accacgcagc cgatgcggcc gggagcgtgc tgatcgccca agagcaccag catgccgccc
     1621 gtttggagca cttcatcggc ctggttggcg ctgtcgctcg tcggcaggat gcggtgcccc
     1681 atcgactgcc ggaaccgcag caccagtcgg tcgaggtagg gattatcgag cggccgcgcc
     1741 accgtataaa cccgcacccc ccagaatccc gccatgaagc cacagatttc aaaattgccg
     1801 aagtgccccg ccacggctac cgtcggccgg cgcgagaacg ccagccgcaa tagctcgcgc
     1861 gagttgtagt cggtcacgtg ctcgcgccag ttcgtgtcgt gcagctttcg ctggacgtgg
     1921 gcgacctcgc agaccatcag gagcaagtgc tcccacatcg cccgcgccag ccgttttcgc
     1981 tcgccctcag tgaggccggg aaacgagatt cgcaggttct catcgacgac gcggcggcga
     2041 attttcagca catcagcagc cagccaggcg acgatgcgaa tgtacgcctg gcaggttttc
     2101 aggctgaccg cctggatcag cgacagcgaa acccggacag ccagatactc ggccagatga
     2161 cgcaagcgtc gcatccctgc actccaaacg ggcgatccct cggcgcggca aatcggctgc
     2221 gtattttgcc cggcttatcg cacaggtcca agagcgattt tcggctttga cggcggcccg
     2281 gccggcgggt attcttcgcc cccgcgcccg cccgtccttt cgttggccca tacccatgcc
     2341 cagcccctcc cgcagtgtgc tgattgtcga tccctcggcc gaaaatcgcg aagtgctgcg
     2401 gacggcactg gagcgtcgcg gcctgcgcat tttcgaggcc gaccaggccg accaggggct
     2461 gcagatggcc cgcgagcacc atccgcacgt gatcgtgctc gatctggaat cggatgagcc
     2521 gcacgccagc gacgtggaag accaatacgg ggccgaggcc cgcgagctgc atacttcgct
     2581 ggtaattctc ggaaaggcct gccgccgaca gcggctggac gccgcccgcc acgtggccaa
     2641 gccctaccac ttcgctccgc tggtccatac aatcgaacag ct
//
LOCUS       DASUGB010000956         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1775246, whole genome shotgun sequence.
ACCESSION   DASUGB010000956 DASUGB010000000
VERSION     DASUGB010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775246"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFV87_18110"
     CDS             complement(<1..382)
                     /locus_tag="VFV87_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5105743.1"
                     /translation="MKYKVTHTTIYNYTEPVPICHNEVHLTPRDHRRQTCLSHRLRIR
                     PEPARLDTRVDYFGNFAGAFSIQEAHDRLAVTAHSHVQVAPPEIPDPAATPPWEEIRD
                     RFALGQPPWWLEARQFVYESPHVPI"
     gene            complement(556..>2825)
                     /locus_tag="VFV87_18115"
     CDS             complement(556..>2825)
                     /locus_tag="VFV87_18115"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX5105744.1"
                     /translation="AAEWSRVSAGLAQRAMLLNMVLADLYGPQRLLAEGLLPPELVYL
                     NPGFLRSCHGWMVPRGRYLYLYAGHLARDQAGQWLVLADKARGPTGAGYAIENRIVIS
                     RMIPDVFRDCQVQRLAAFFLSMRDTLQELVPTRGTPRIVLLSPGPTSPTYFEDAYLAR
                     YLGFALVEGGDLTVRDNCVYLKTLGGLLQVDVILRRVRDEDCDPLEQRSDSTHGVSGL
                     THAARSGNVALVNALGCSLLESPAVIPFLPTLAKFFLREDLLLPSVPSWWCGDDAARE
                     FVLSNLETLVIKPSFPQPAIGPIYGARLTGQQRDDLRARIRHQPQDYVAQELIAGSTA
                     PAWNSGAVQPTYVALRTFAFVKDEHYDVMPGGLARVSLSPDALGESALGGQGSKDVWV
                     LSEGPVADVTLLHPAGAPISLQRSTSDLPSRVADHLYWLGRHAERTEGVARLVRSLVA
                     RLTSEGATGSSPSARALLRALSDQGRVRPEFAVHLGGQKIPQIEHEILAAIFDPTRTG
                     SLRAMLTALHRAAAVVRDRISIDSWRILNSLALELAPPSSADAQVALADVLSLLNGLI
                     LDLSAFSGMGTESMTRGPGWRFLDMGRRIERGQQGLSLLRSTLVEPQADEDPVFEGLL
                     EIADSSMTYRNRYLTNLQLPPLLDLLLTDETNPRSVAYQASALNDHVEALPRGASQLL
                     LASEQRVALALLTDLRLSDVYALAKLDASGERTMLHEFLENCAAHFRTLSDEITTHYL
                     IHAGPSRQIGEIRPG"
BASE COUNT          509 a          881 c          969 g          466 t
ORIGIN      
        1 tgatcggcac gtgcggcgat tcatacacga actgccgggc ctccagccac cacggcggct
       61 ggccgagggc gaagcggtcg cggatttcct cccagggggg cgtggcagcg gggtcgggaa
      121 tctccggcgg ggcgacctgc acgtggctgt gggccgtcac cgccagccgg tcgtgagctt
      181 cctgaattga gaaggcgccc gcgaaattgc cgaagtaatc gacccgcgta tcgagccgcg
      241 ccggctccgg ccgaatccgc agccggtgcg aaaggcacgt ctgccgccga tgatcgcgcg
      301 gcgtgagatg cacctcgttg tggcagatgg ggaccggctc ggtgtagttg taaatcgtcg
      361 tgtgggtgac tttgtatttc atcggctgcg catcctgctc gtaatcttgc tcttgctcta
      421 aatcgtcgtc ctaatcgtaa tcctgctctt tctcttactc ccgatccggc caggccggcg
      481 acaggagtaa gagcaagagc aggagcaaga ttaggaagag gattaagatt aggagtagga
      541 gtaggattag gacttttatc cgggccggat ctccccgatc tgccgtgaag gaccagcgtg
      601 gatcaggtag tgcgtcgtga tttcgtccga gagcgtgcgg aagtgggccg cacagttctc
      661 taggaactcg tgaagcatgg tgcgctcgcc gctggcatcg agtttcgcca gggcatagac
      721 atctgacagc cgcaggtcgg tcaagagcgc cagggcgacg cgctgctcgc tcgccaggag
      781 gagctgcgaa gcgccgcggg ggagcgcttc gacgtggtcg ttcagggccg aggcctggta
      841 ggcgaccgat cgcgggttcg tttcatccgt gagcagcaag tccaagagcg gcggaagctg
      901 caggttcgtg agatagcggt tgcggtaggt catcgagctg tcggcgattt cgagcagccc
      961 ctcgaagacc ggatcttcgt cggcttgcgg ctcgacgagc gtgctccgca ggagcgacag
     1021 cccttgctgg ccgcgttcga tccggcggcc catatcgagg aaccgccagc cgggaccgcg
     1081 ggtcatgctc tcggtcccca tgccgctgaa ggcggacagg tcgagaatca gcccgttgag
     1141 gagcgacagc acgtcggcca gcgcgacctg ggcgtcggct gatgaaggcg gcgccagttc
     1201 gagcgccagg ctgttgagaa tccgccaact gtcgatcgaa atccggtcgc gcactaccgc
     1261 ggctgcccgg tgcagagcag taagcatcgc ccgcaagctg ccggtgcggg tgggatcgaa
     1321 aatagctgcc agaatctcat gctcgatctg cggaattttc tggccgccga gatggaccgc
     1381 gaactcgggc cgcacgcggc cttgatcgga cagcgcccgc agcaaggccc gggcgctggg
     1441 cgagctgccg gtcgctcctt cgctcgtcag ccgggccacg aggctccgca ccaggcgggc
     1501 cacgccctcg gtccgctccg catggcggcc gagccaatag agatggtcgg ccacgcggct
     1561 ggggaggtcg ctggtgctcc gctgcaggct gatcggcgct ccggcgggat gcagcagggt
     1621 cacatccgcg acgggcccct cgcttagcac ccacacgtcc ttgcttccct gcccgccgag
     1681 agccgattcg ccgagcgcat cgggcgaaag cgacacgcgg gccaggccgc cgggcattac
     1741 gtcgtagtgc tcgtccttga caaaggcgaa cgtgcgcaaa gcaacgtacg ttggctgcac
     1801 cgctccactg ttccaggccg gcgctgttga gccggcgatc agctcctgtg cgacatagtc
     1861 ctgcggctgg tggcggatgc gggcccgcag gtcgtcacgc tgctgcccgg tcagccgggc
     1921 gccgtaaatg ggcccgatcg ccggctgagg aaagctgggc ttgatgacga gcgtttcgag
     1981 gttcgagagc acaaattcac gagccgcatc gtcgccgcac caccagctcg gcacggaggg
     2041 cagcagcaga tcctcgcgca ggaagaattt cgccaaggtc ggcaggaatg gaatcaccgc
     2101 cggcgattcg agcaggctgc agccgagcgc gttcaccagg gccacattgc cgctgcgggc
     2161 ggcgtgagtc agtccggaga cgccatgcgt cgaatcgctg cgctgctcca gcgggtcgca
     2221 gtcttcgtcc cgaacgcggc ggaggatcac atcgacctgc aatagtccgc ccagcgtctt
     2281 cagatagacg cagttgtcgc ggaccgtcag atcgcctcct tcgaccagcg caaagccgag
     2341 atagcgggca agataggcgt cctcaaagta ggtcgggctg gtcgggccgg ggctgagcag
     2401 cacgatccgc ggcgtgccgc gcgtcggaac gagctcctgc agcgtgtcgc gcatcgacag
     2461 gaagaacgct gccagccgct gcacctggca atcacggaac acgtcgggaa tcatccgcga
     2521 gatgacgatg cgattttcga tcgcatagcc cgcgccggtc ggcccgcggg ccttatcggc
     2581 gagcacgagc cattggccgg cctgatcgcg ggcgagatgg ccggcgtaca ggtagaggta
     2641 acgcccacgc gggaccatcc acccatggca cgatcgcaga aagcccggat tcagatagac
     2701 cagctccggt ggcagcagcc cttcggccaa gaggcgctgc gggccataaa ggtccgccag
     2761 aaccatgttg agcagcatcg cccgctgtgc aaggccggcg ctgacgcgcg accattcggc
     2821 agccg
//
LOCUS       DASUGB010000957         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1219381, whole genome shotgun sequence.
ACCESSION   DASUGB010000957 DASUGB010000000
VERSION     DASUGB010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219381"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFV87_18120"
     CDS             <1..386
                     /locus_tag="VFV87_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105745.1"
                     /translation="LLCVLITLFSVGLMGEAQRQQICNSKSGYYIAVLLDRADAVIPG
                     ELHQLLEPYIDQLDRSVAHPHTEEGWQIPREIQDLIPQPGQTSGQPPAGGSFPTGGFG
                     LPAEEPQLPRSAGPIEARPAQPFNY"
     gene            complement(511..1533)
                     /locus_tag="VFV87_18125"
     CDS             complement(511..1533)
                     /locus_tag="VFV87_18125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5105746.1"
                     /translation="MSNDAPPTVTTPGLDFEQPLLDLEGRRRALEQITDRSSEQADQL
                     RQLRRELADKTREIYAGLTPWQIVQVARHKDRPYTSDYLSLVFEDFVELHGDRRYGND
                     RAILTGFARLDQHKVLVVGHQKGRTYKERSACYFGCAHPEGYRKAILKMKLAEKYGLP
                     VICLIDTPGAYPGIGAEERGQSQAIAENMYEMSRLKTPIICVVIGEGGSGGALGIGVG
                     DRVAVLEFSYYSVISPEGCAGILWKSHEFAERAATALKFTSKDLLRLGVVDHVIEEPL
                     GGAHRDQHQTAMRVKQYLLRSLRELLAQPTDKLLTGRYEKFRRMGVFLDDPAASAKSN
                     GQSVLS"
     gene            complement(1605..2498)
                     /locus_tag="VFV87_18130"
     CDS             complement(1605..2498)
                     /locus_tag="VFV87_18130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105747.1"
                     /translation="MSRPRDFLGPYRLVRLIRVGHTCQVWEAAKTDDTTRYVLKVLRP
                     DLRKDRHELAAMKNEFECGKALKHPNIIRIYDHKTDGEAPYLVMEHFEHNNLKLWLRN
                     GPQPVAHLSPKIIEQSAESLFYMHSEGWIHCDVKPDNFLVSDEGEVKLIDFAISQRQK
                     KGLSAILSFGKKVQGTRSYMSPEQIRGKTLDPRSDIYSYGCVLFELLTGKPPFTGTTA
                     DELLNKHLTAPIPSVQVYNDNTTSEMANLLRKMMAKTREERPNTTWEFLKEFRSIRAF
                     KVVPKAPVTKPDLKEERKGFI"
     gene            complement(2624..>3841)
                     /locus_tag="VFV87_18135"
     CDS             complement(2624..>3841)
                     /locus_tag="VFV87_18135"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5105748.1"
                     /translation="HSAFCILHSEFCISSMRIAHLITRMIVGGAQENTLYNCEDLIRL
                     HGDEVLLVTGPAIGPEGDLLQQGRGGEGSVEVPKRIIRSLRRAIVPWQDTSSYFALKQ
                     VIREFQPDVVHTHSAKAGFLGRLAAWKLRVPAIVHTVHGAPFHAYQSAAARSLFRACE
                     RYAAKRCHALISVADAMTDLMAQANVAPREQFTTIYSGIDVEPFLRADERREQTRREL
                     GFAPEHVVVGKIARLFHLKGHADVIRAAAEVIASQPNVRFLFIGDGILRERLQSQIDA
                     AGLHEHFRFAGLVPPSDIPQHIGAMDLLVHASLREGLARALPQALIAGKPVVSYDVDG
                     AREVAIADQTGFLVAPQWQNLVQPLIALAADADLRKRLGSEGRRRFTDQFRHEFMTQR
                     IRELYERVLNQPR"
BASE COUNT          688 a         1166 c         1245 g          742 t
ORIGIN      
        1 tgctactgtg cgtgctgatc acgctgtttt cggtggggct gatgggcgag gcccagcggc
       61 agcaaatctg caactcgaaa tccggttatt acatcgctgt gctgctcgac cgggcggacg
      121 cggtcattcc cggcgagttg caccaactgc tggagccgta catcgaccag ctcgaccgca
      181 gcgtggcgca tccgcacacg gaggaaggct ggcagattcc gcgcgagatc caggacctga
      241 ttccgcagcc cggacagact tccggccagc caccagcagg gggtagcttc ccgaccggcg
      301 gcttcggact cccggccgag gagccccagt tgccccgctc cgccggcccc atcgaagccc
      361 ggccagccca accgttcaac tactgaagcc agcgaaagtg cgcgcagcag cgaaaacgcc
      421 cagtcgcacc ttccagcggg aaaacccgcc gcgcaaccga acataacaaa cattatcgga
      481 cgttgggggg aggcctctct acgggcccaa tcagctcaat acggactggc cgttggactt
      541 cgcactggct gctggatcat ccaggaagac gcccatccgg cgaaatttct cgtagcggcc
      601 cgtcagcagc ttatccgtcg gctgggccag cagctctcgt aacgagcgga gcagatactg
      661 cttgacccgc atggccgtct ggtgctggtc gcggtgggct ccgcccagcg gctcttcgat
      721 gacgtgatcg accacgccca gccgcaatag atccttggac gtgaatttga gtgccgtggc
      781 ggcccgttcg gcgaattcgt ggctcttcca gaggatgccc gcgcagccct cgggactgat
      841 gaccgagtaa taggagaact ccaggacggc cacacggtcg cccacgccga tgccgagcgc
      901 tccaccgctt cccccttcgc cgatgacgac gcaaatgatc ggcgttttca gccgcgacat
      961 ctcgtacatg ttctcggcaa tggcttgcga ttggccgcgc tcttcggcgc caatacccgg
     1021 ataggctccg ggtgtatcga tcaggcagat gaccggcaac ccgtacttct cggccagctt
     1081 catcttgagg atcgccttgc gatatccctc cgggtgtgcg cagccgaagt agcaagcgct
     1141 ccgctccttg taggtcctcc ccttttgatg gccgacgacg agcaccttgt gctggtcgag
     1201 ccgcgcgaag cccgtgagaa ttgcccggtc gttgccatag cgccggtcgc cgtgcaactc
     1261 gacaaagtct tcgaacacga gcgacaggta gtccgaagtg taggggcgat ccttgtgccg
     1321 ggcgacttgg acgatttgcc agggcgtcag gcctgcgtag atctcgcgag tcttgtccgc
     1381 caactcgcgc cgcagttgcc gcagctggtc ggcctgctcg gacgagcggt cagtgatttg
     1441 ctctagtgcg cggcggcggc cttccaggtc caagagcggt tgctcgaaat cgaggccggg
     1501 cgtggtgacg gtcggtggtg cgtcgttcga catagggacg gagccgggac caaagaatcg
     1561 gaccaactgc ggtagtgtgc ctcaacctcg cgcggccagc aacctcaaat gaaacccttc
     1621 cgctcttctt taagatcggg cttcgtgacg ggcgctttgg gaacgacctt gaaagcgcgg
     1681 atgctgcgga actccttgag aaactcccaa gtcgtgtttg gacgttcctc gcgggtcttg
     1741 gccatcatct tccgcagcag gttggccatc tcggacgtgg tgttgtcgtt ataaacctgg
     1801 accgacggaa tcggggcggt gaggtgcttg ttgagcagct catccgcggt tgtgccggtg
     1861 aacggcggct tgcccgtcag cagctcgaac agcacgcagc cgtagctgta gatatccgag
     1921 cgcgggtcga gcgtcttgcc gcgaatctgt tcgggcgaca tgtagctgcg ggtcccctgc
     1981 actttcttcc cgaacgacag gatggcggac agcccctttt tctgccgctg ggagatggcg
     2041 aagtcgatca gcttgacttc accttcgtcg ctgacgagga agttgtcggg cttcacgtcg
     2101 cagtgaatcc agccttcgct gtgcatgtag aacagcgatt cggcgctctg ctcgatgatc
     2161 ttgggcgaca agtgggcaac gggctgcggg ccgttccgca gccagagctt gaggttgttg
     2221 tgctcgaagt gctccatcac caggtagggc gcttcgccgt cagtcttgtg atcgtaaatg
     2281 cggatgatgt tcggatgctt cagcgccttg ccgcactcga actcgttctt catcgcggcc
     2341 agttcgtggc ggtcctttcg caagtcggga cgcagcacct tgaggacgta ccgcgtcgtg
     2401 tcgtcggtct tggcggcttc ccacacttgg catgtatggc cgacgcgaat caatcgcacg
     2461 aggcgataag gaccgagaaa gtcgcggggt cgggacacgt tagcgcctgg tcagtgaacg
     2521 gtgtcgatgc aaggcattag ggacgaggtg gttgggccgg ttgtagttta agcggactgg
     2581 cagcggggcg aaagagtcgg ctcgcggcgc tcttgacttg atcttaacgc ggctgattca
     2641 gcacgcgctc gtacaattcg cggatccgct gggtcatgaa ctcgtggcgg aactggtcgg
     2701 tgaatcgccg gcggccttcg gacccgagac gcttgcgcag gtcggcatcg gcggccagtg
     2761 caatcagcgg ctggacgagg ttttgccact gtggagcgac gagaaaaccc gtctggtcgg
     2821 caatggccac ctcgcgggcg ccgtccacgt catagctgac gaccggcttg ccggcgatga
     2881 gcgcctgcgg caaagcgcgg gcgaggcctt cgcgcagact tgcatggacg agcaggtcca
     2941 tcgcgccgat gtgctgggga atgtcgctcg gcggcaccaa gccggcgaag cggaagtgct
     3001 cgtgcagtcc agccgcatca atctgcgact gcagccgctc gcgcaggatg ccgtcgccga
     3061 tgaagagaaa gcgcacattc ggttgcgacg cgatcacttc cgcggcggcc cggatgacat
     3121 cggcgtgccc tttcaggtga aacagccggg cgatcttgcc gacgacgacg tgctcgggcg
     3181 cgaaccccag ttcgcggcgg gtttgctcgc gacgctcgtc ggcgcggaga aacggctcga
     3241 cgtcgatgcc gctgtagatt gtcgtgaatt gctcgcgcgg agcgacattc gcctgggcca
     3301 tcaagtcggt catggcgtcg gcgacgctaa ttagcgcgtg gcaccgcttt gccgcatagc
     3361 gctcgcacgc gcgaaacagg ctccgcgccg ccgcactttg gtaggcgtgg aacggtgccc
     3421 cgtggaccgt gtggacgatc gccggcacac gcagcttcca cgcggccagc cggccgagaa
     3481 agccggcttt ggcgctgtgc gtgtggacga catcaggttg gaactcgcgg atgacttgct
     3541 tcaaggcgaa gtagctggac gtgtcttgcc agggaacgat ggcgcggcgc aggctgcgga
     3601 tgattcgctt cggcacctcc acgctgccct cccctcccct gccctgttgg agcaagtcgc
     3661 cctccggtcc aatcgccggg ccggttacga ggagcacttc atcgccatgc agacgaatca
     3721 aatcttcgca gttgtaaagc gtgttttcct gcgcgccgcc gacgatcatg cgggtgatca
     3781 ggtgggcgat gcgcatggag gaaatgcaga attcggaatg cagaatgcag aatgcagaat
     3841 g
//
LOCUS       DASUGB010000958         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_802463, whole genome shotgun sequence.
ACCESSION   DASUGB010000958 DASUGB010000000
VERSION     DASUGB010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802463"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFV87_18140"
     CDS             complement(<1..288)
                     /locus_tag="VFV87_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105749.1"
                     /translation="MKIRFLVAAIILLGPAVPVGTCLARGFGGGGFGGGFHGGGLGGG
                     LGGGGFGGARMGGGFGGGLGGVGLGGLGGGGFDRGGLGGLGGGGLGGLGGGG"
     gene            complement(363..1271)
                     /locus_tag="VFV87_18145"
     CDS             complement(363..1271)
                     /locus_tag="VFV87_18145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5105750.1"
                     /translation="MILPHLTVIAALLLTAGVAGQAVAQAPPVTKATTAEAALRQSSQ
                     AFAEAFNQGDANAVAALWTEDGDYIDEAGRTFAGRKAIEKSYAAFFAAHPGVKIKVVV
                     DSIKLLGDSAAIEDGRAFLEPAPGAPAISKYTAAHVKIGSQWLMATVRDSRIELPSGY
                     SKVADLEWLIGQWIAEEHGTKTESHCRWVANKSFVERKYTTTSHDGIASAGVQLIGWN
                     AHGGHVQSWNFSPDGGHAIGIWTPREGGWEAEIRGATGDGVNTTATNILTKLDDNAYA
                     WQSINRTAGGQPLPDSDEVIVKRNQQ"
     gene            complement(1430..2671)
                     /locus_tag="VFV87_18150"
     CDS             complement(1430..2671)
                     /locus_tag="VFV87_18150"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5105751.1"
                     /translation="MFVVTACGFSLSAQEIAATGDAVPELSGFDEWMKAFMAEHEIPG
                     GQLAIVRHGKLIYARGFGWADRDANESVQPESLFRIASVSKPITAVAILKLVDQGKLK
                     LDDRILDHLTYEPHLEEGGQFDERWNQVTIRHCLSHAGGWDREKSYDPMFQAVRMAKS
                     MQVDLPILPEHVIRYQLGQPLDFDPGERYAYSNFGYCLLGRVIEKVTGQPYEKYVQEE
                     VLKPLGISRARIGGSLEDQRAEGEVKYYDVKGEPGKAIVGPGAGEKEVPVSYGTWRQE
                     TLDSHGGWIASAPDLAKFAGAFDFNDSGDGTRSGLLKRDSVHDMIAAHVQISPATDDK
                     PARSYGLGWFIEQLPGENAVIVRHGGALPCTAASLMHFPDGTNLAVLFNLGQDKDRKF
                     LGRSIEGPLTDLVRSVGTWPQ"
     gene            2793..>3483
                     /locus_tag="VFV87_18155"
     CDS             2793..>3483
                     /locus_tag="VFV87_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX5105752.1"
                     /translation="MSQYPHIFRVRQTFNSPTLPVKEIPATVAAELTRLKLNAVVKPG
                     QSVAITAGSRGIANIALVIKAVVEHFQSLGAKPFIVPAMGSHGGGTAEGQRGLIEGYG
                     ITEEYCGCPIRASMETVIVCQAAEGFPVHFDKQAFGADHVVVVGRVKPHTNFVGDIES
                     GLMKMMLIGLGKHEGAKIYHRAIMDFNFGQIVRSVAREVLAKCRIVAGLGIVENAYDQ
                     TAKIQAVAPHEF"
BASE COUNT          661 a         1091 c         1079 g          652 t
ORIGIN      
        1 gcccccgccg cccaaaccgc cgaggcctcc gccgcctagg ccgccaagcc ctccgcggtc
       61 aaatcctccg ccgcctaagc caccaaggcc gacgcccccg aggccccctc caaatcctcc
      121 acccatcctt gcgccgccga agccgccccc acccagtccg ccgcccaagc cgccgccgtg
      181 aaatcctcca ccaaaaccac caccgccaaa gccgcgcgcc aggcaggtgc caacggggac
      241 ggcagggcca agcaaaatga ttgcggcaac aaggaatcga attttcatcg ggcggctcac
      301 attcgggcag gaggatttgg tgctcaggaa acgtcctcgg acgaacgggt tggaagcccg
      361 ttctactgtt gattgcgttt gacgatgact tcgtccgagt ccggcaatgg ctggccgccg
      421 gcggtgcggt tgatcgattg ccaggcgtag gcgttgtcgt cgagcttcgt caggatgttc
      481 gtcgcggtcg tgttgactcc atcaccggtg gcgccgcgaa tctccgcttc ccagccccct
      541 tcgcggggcg tccagatgcc gatcgcgtgg ccgccgtccg ggctgaaatt ccaagactgc
      601 acatggccgc cgtgggcatt ccagccaatg agctgcacgc ccgctgaggc gattccgtcg
      661 tggctggtgg tcgtgtactt ccgctcgacg aagctcttgt tggcaaccca gcggcagtgg
      721 gattcggtct tcgtaccgtg ctcctccgcg atccactggc cgatgagcca ttccaaatcg
      781 gcaactttgc tgtagccgga ggggagttcg atgcgcgagt cgcgaacggt cgccatcagc
      841 cactggctgc cgattttgac gtgagccgcc gtgtacttgc tgatggccgg agcgccggga
      901 gcaggctcga ggaaggcccg gccatcttcg atcgcggcac tatcgcccag gagcttgatc
      961 gaatcgacga cgaccttgat cttcacgccg ggatgggcgg cgaagaaggc ggcgtacgat
     1021 ttctcgatcg ccttgcgacc ggcaaaggtg cgacccgcct catcgatata gtcgccgtct
     1081 tcggtccaga gtgccgcgac tgcatttgcg tcgccttggt tgaatgcctc cgcaaacgcc
     1141 tgggaggact ggcgaagggc ggcttccgca gtagttgcct tggtgaccgg gggggcctgg
     1201 gctacggctt gtccggccac ccccgcggtc agcaggagcg cagcaattac cgtcagatgt
     1261 gggagaatca tggtgcgtgc tcacaagatg cgtggggggc cgacgaccgt cagccaagtg
     1321 acaaagggta gtatcccacg aaaacagctc gaatgaagtg cccgtccaga caaattgcgc
     1381 agaatttaac gtcccgctaa cgaaagccag ttcctgacaa gcgaacgcat tattgcggcc
     1441 aggttcccac cgatcgaacc agatccgtga gcgggccttc gatgctgcgg ccaaggaatt
     1501 tgcgatcctt gtcctggccc aaattgaaga gcacggccag attcgtgccg tcaggaaagt
     1561 gcatcagcga agcggctgtg caggggagcg cgccgccgtg acggacgatg acggcgtttt
     1621 cgcccggcag ttgctcgatg aaccagccca ggccatagga gcgagccggt ttgtcgtcgg
     1681 tggcgggaga gatttggacg tgcgcggcga tcatgtcgtg aactgaatcg cgtttcagca
     1741 acccggaacg cgtgccgtcg ccggaatcgt tgaaatcgaa ggccccggcg aacttcgcca
     1801 ggtctggcgc gctggcaatc cagccgccgt gggaatcgag cgtctcctgc cgccaggttc
     1861 catagctgac cggcacttcc ttttcgcccg cgcccgggcc gacgatcgcc ttgcctggct
     1921 cgcctttgac gtcgtagtac ttcacttcgc cctcagcccg ctgatcctcc agcgagccac
     1981 caattctggc ccgagagatg cccagcggct ttagcacttc ctcctgcacg tacttctcat
     2041 agggctgccc cgtgaccttc tcgatcacgc gccccagcag gcagtagcca aaattggaat
     2101 aggcatatcg ctcgccggga tcgaaatcga ggggctggcc gagttgatag cggatgacgt
     2161 gctccggcag gatcggcagg tcgacctgca tcgactttgc cattcgaacg gcctggaaca
     2221 tcggatcgta cgacttctcg cggtcccagc cgccagcgtg cgagaggcag tgacggatcg
     2281 tgacttggtt ccagcgctcg tcgaattgtc ccccttcctc gaggtgcggt tcgtaggtga
     2341 gatggtcgag aatcctgtca tcgagcttca gcttgccctg atcgaccagc ttcagaatcg
     2401 ccaccgccgt gatcggcttg ctgacgctgg cgatgcggaa cagggactcc ggctgtacgg
     2461 actcgttcgc gtcgcgatcg gcccagccaa acccgcgggc atagatcaat ttgccgtgcc
     2521 gcacgatcgc caactgcccg ccgggaatct catgctccgc catgaaggcc ttcatccact
     2581 cgtcgaagcc gctcagctca ggcacggcat ccccggtggc ggcgatttct tgtgcggaca
     2641 gtgagaaccc acatgctgtg acgacaaaca tgacggtcag ttggataagc ggcttcatgg
     2701 tcgggtgagc tggcggtaaa tggtcctgac gttggattgg actccaatct ataatccagg
     2761 caagtgattg ggaaccattt tcgcgaatcg ccatgtcaca atatccccac atcttccgag
     2821 tccgccaaac cttcaactcg cctacgctgc cggtcaagga gattccggcg accgtcgctg
     2881 cggaactcac tcggctgaaa ctcaatgcgg tcgtgaagcc gggacagagc gtcgcgatta
     2941 ccgccggcag ccgggggatt gcgaacatcg ccctggtcat caaagcggtg gtggagcatt
     3001 ttcagtcgct gggggccaag ccgttcattg tgccggcgat ggggagccac ggcggcggca
     3061 cggcggaagg gcaacgcggg ctgatcgagg gctacggcat taccgaggaa tactgcggct
     3121 gcccgatccg ggcgagcatg gagacggtga tcgtctgcca ggcagccgaa ggctttccgg
     3181 tgcatttcga caagcaggcg ttcggggccg atcacgtggt ggtcgtcggc cgcgtcaagc
     3241 cacacacgaa cttcgtcggc gatatcgagt cgggcttgat gaagatgatg ctgatcggcc
     3301 tgggcaaaca tgaaggggcc aagatctatc accgggcgat catggacttc aacttcgggc
     3361 agatcgtccg cagcgtcgcc cgcgaagtgc tggccaagtg ccgcatcgtc gccggcctcg
     3421 ggatcgttga gaacgcttac gatcagacgg cgaaaatcca ggcggtagcc ccgcacgaat
     3481 tcg
//
LOCUS       DASUGB010000959         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1219623, whole genome shotgun sequence.
ACCESSION   DASUGB010000959 DASUGB010000000
VERSION     DASUGB010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219623"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFV87_18160"
     CDS             <1..882
                     /locus_tag="VFV87_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX5105753.1"
                     /translation="VLFTPHWPEVEAKFGQRDWESADGVVNWCREKNVRLCCGPLVLL
                     DRHALPDWVFLDNDEEEVQTSVLSWLEAVVERYRGKVQIWHVAARLNQDGAFDFSEEQ
                     RLRLLVEAVDRVRALDARTPLVVSFDQPWGEYIARKDQELTPLHFADTLVRGELGLAG
                     VGLEIHYGYWPGGTLPRDPLDVSRQLDRWSQLGVPLMVTLSAPSSLAADLRALHSAKP
                     LPDLSAEKLSPKWQQDLIQWLLPMIASKPMVQAIVWDAWQDNAPHEFAQSGLLDAKGK
                     SKPALQTMIDLRRDSIG"
     gene            994..2013
                     /locus_tag="VFV87_18165"
     CDS             994..2013
                     /locus_tag="VFV87_18165"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5105754.1"
                     /translation="MRISTAVFFLLFLLSSIAWAQPKAAVTDPALADADFFLQGEYVG
                     WVSGTSVGLHVVALGKGQFEATGYLGGLPGEGWDKLGRNAYRGTADDGALILEGPAGR
                     FIVAGGFATPIDTSGQTLGRLRKVIRISPTLGLQPPSSATVLFDGSSPEHLQGAKITD
                     DGLLQAGVLTKMPVGPFRLHLEFRTPYMPQAKGQARGNSGVYIQQRYEVQILDSFGLE
                     GVENECGGLYKQQRPDVNMCLPPLSWQTYDIWFTPPRFDEAGKKTANARLTVLLNGYL
                     IHHHREITAKTGGGKPEGPQDLPINLQDHGNPVTFRNIWIVPGDGDENYSLSRYLTRN
                     LRNWN"
     gene            complement(2020..3030)
                     /gene="trpD"
                     /locus_tag="VFV87_18170"
     CDS             complement(2020..3030)
                     /gene="trpD"
                     /locus_tag="VFV87_18170"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5105755.1"
                     /translation="MITSALGRLAAGDNLSLEEMAEVIDQVMSGRVPENEIAVLLTAL
                     RHKGETIDEIAGAALAMRRHMTPIRTARTGLVDTCGTGGDASGTFNISTAAAIVASAA
                     DVPVAKHGNRSITSKSGSADVLAALGVNVGAPVPVVERCLSELGICFCFAPHLHPAMK
                     HVAPVRKKLGVPTIFNLLGPLCNPAGAPFQVLGVGKAELHQTLADVLARIGIRRAVVV
                     HGLDGLDEVSLSGPTDVIEITGHTTRRFVWHPSDFGLAPAGRESMLIDGPAASAALIR
                     RILDGERGPPRDIVLLNAAAALWTAGISESPLECANRAAETIDSGAAGKLLGRWGALS
                     NE"
     gene            3157..>3456
                     /locus_tag="VFV87_18175"
     CDS             3157..>3456
                     /locus_tag="VFV87_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5105756.1"
                     /translation="MPRKIIIDCDPGIDDLVALTLALFDPRLEVVAITAVAGNVSAEQ
                     ATINVQAIVERLDPPRYPRLGAATPAGDFAYLDSRRLHGPDGLGGTGLAVSQLARQ"
BASE COUNT          622 a         1143 c         1077 g          614 t
ORIGIN      
        1 gtcttgttca cgccgcattg gccggaagtc gaagccaaat tcggacagcg cgattgggag
       61 tcggcggatg gcgtcgtgaa ctggtgccgc gagaagaacg tgcggctgtg ctgtggtccg
      121 ctggtgctgc tcgatcgcca cgccttgccc gactgggtct ttctcgacaa cgacgaggaa
      181 gaggtgcaga catccgttct ctcctggctg gaggcggttg tcgagcgcta tcgcggcaag
      241 gtgcaaatct ggcatgtggc ggcgcggctc aatcaggacg gggcgttcga cttctcggag
      301 gagcagcgac taaggctgct ggtggaagcc gtcgatcgcg tgcgggccct cgatgcccgg
      361 acgccgctgg tcgtcagctt cgatcaacct tggggcgaat acatcgcccg caaagaccag
      421 gagctgacgc cgctgcactt tgccgacacc ctcgtccgcg gcgagttggg gctggcgggc
      481 gtcgggctgg aaatccacta cggctactgg ccaggcggca cgctcccgcg cgacccgctg
      541 gacgtcagcc gtcagctcga ccgctggtcg cagcttggcg tgccgctgat ggtgacgctc
      601 tccgctccca gcagcctggc agccgatctc cgggcgctgc attctgccaa gccgctgccc
      661 gacctgtcgg ctgagaagct ctcgcccaag tggcagcagg atctgatcca atggctgctg
      721 ccgatgatcg cctctaagcc gatggtgcag gcgatcgtct gggacgcctg gcaagacaac
      781 gccccgcatg aattcgccca aagcggcctg ctcgacgcca aaggcaagtc caagccggcc
      841 ttgcagacga tgatcgacct ccgccgcgat tcaatcggct agctgaggcc ttttccactg
      901 cccggcaagc agagcggatc gactggaagt atgcttgtac tggcacaaaa ccgcgacaaa
      961 accagcgccc cactatcgac cacgagcgac accatgcgca tctctactgc cgtgttcttc
     1021 ttgcttttcc tgttgagttc gatagcctgg gctcagccca aggcagccgt caccgatccg
     1081 gcgttggccg atgccgactt tttcctgcag ggcgagtacg tcggctgggt aagcggcacg
     1141 tcggtcggct tgcacgttgt agctctcggc aagggccaat tcgaagcgac gggctatctc
     1201 ggcggcttgc ccggcgaagg ttgggacaag ctgggcagga atgcctaccg tggcacggcc
     1261 gacgacggcg cgctcattct cgaaggaccc gcaggacgct tcatcgtggc cggaggattt
     1321 gccacgccga tcgacaccag cggccaaacg ctcggacgac tccgcaaagt catccgcatc
     1381 agcccgacgc tggggctgca accgccgtcc agcgccacgg ttctctttga cggcagctcg
     1441 cccgaacatc tgcagggcgc gaagatcact gacgatggcc tcctccaagc cggcgtgctg
     1501 acgaaaatgc cggtcggtcc cttccgcctg cacctggaat tccgcacgcc ttatatgcca
     1561 caggccaagg gccaggcccg cggcaacagc ggcgtctata tccagcagcg ctacgaggtc
     1621 cagattctcg attcattcgg cctggaaggc gtcgagaatg aatgcggcgg gctgtataag
     1681 caacagcggc cggacgtcaa catgtgcctc ccgcctcttt cttggcagac ttacgacatc
     1741 tggttcaccc cgccgcggtt tgacgaagca ggcaaaaaga ccgccaacgc ccgccttacg
     1801 gtcctcctca acggctatct gattcatcat caccgcgaga tcactgccaa aaccggtggc
     1861 ggcaaacccg aaggcccaca ggacttgccg atcaacctgc aagaccacgg caacccggtc
     1921 acgttccgca atatctggat cgtacccggc gacggggatg agaattacag cctctcgcgc
     1981 tatttgacgc gcaacttgcg aaactggaac taagcgctct tactcattgc tgagtgcgcc
     2041 ccagcgacca agcaatttcc cggccgctcc ggaatcgatc gtttcagcgg cgcgattggc
     2101 gcattcgagt ggcgactcgc tgattcccgc ggtccataga gccgccgccg cgttgaggag
     2161 gacgatatcg cgcggcgggc cacgctcgcc gtccaggatt cggcggatca gcgcagcgct
     2221 ggcggcgggg ccgtcgatca gcatgctttc gcggccggct ggcgcgagac caaagtcgga
     2281 ggggtgccat acgaaacgcc gtgtcgtgtg gccggtgatc tcgatcacat cggtcggacc
     2341 gctgagcgaa acctcatcga ggccatctag gccgtggacg acgacggccc gcctgatgcc
     2401 gattcgcgct agcacgtccg ccagcgtctg gtgcagttcc gccttgccga cgccgagcac
     2461 ctgaaacggc gcaccggctg gattgcagag cggtccgagc aggttgaaga tcgtcggcac
     2521 gccgagcttc ttccgcaccg gcgcgacgtg tttcatcgct ggatgcaggt gcggagcaaa
     2581 gcaaaagcag atgcccaact cgctgagaca gcgctcaacg accggcacgg gcgcaccgac
     2641 gtttactccg agcgccgcca gcacgtccgc cgagccgctc ttgctcgtga tgctgcggtt
     2701 gccgtgcttg gcgacgggca catccgccgc ggaggcgaca atcgccgcgg ccgtgctgat
     2761 attaaacgtg ccgctggcgt cgccgccggt cccgcacgta tcgaccaggc ccgtccgcgc
     2821 ggtgcggatc ggcgtcatgt gccgccgcat ggccagggcc gcaccggcga tctcatcgat
     2881 tgtctctccc ttgtgccgca gtgccgtgag cagcaccgca atctcgttct ccggcacacg
     2941 gccgctcatc acctggtcga tcacttcggc catttcttcg agcgacagat tgtcgccggc
     3001 tgccaggcgg ccgagagcag aagtgatcat gcggcttcgg cgaaagggag gaaagcaact
     3061 cattcgattc tatcagcccg gccaatccct tgttgggcgg gccgagcagt cctaaaatcg
     3121 tttgagcgct aacgatctgc actcgccagc cacaccatgc cgcgcaaaat catcatcgac
     3181 tgcgatccgg gcatcgacga cctggtcgcg ctgaccctgg cactcttcga tccgcggctg
     3241 gaggtggtgg ccatcaccgc ggtggcgggc aacgtctcgg ccgagcaggc caccatcaat
     3301 gttcaagcga tcgtcgagcg gcttgatccg ccgcgttatc cgcggctggg ggcggcgacg
     3361 cctgccgggg acttcgccta ccttgactcg cgccgcctgc acggtcccga tggcctcggc
     3421 ggcacgggcc tggccgtctc gcagcttgcc cgccag
//
LOCUS       DASUGB010000960         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1776474, whole genome shotgun sequence.
ACCESSION   DASUGB010000960 DASUGB010000000
VERSION     DASUGB010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776474"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFV87_18180"
     CDS             <1..862
                     /locus_tag="VFV87_18180"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5105757.1"
                     /translation="GYGTGLIVSPQGDILTAQGVYLAGDSLRVTLPDGTTHYATVQRR
                     SDALQAAVIKIEAETPDYFDLPAQPADGSAARPAAEQGDWILAVSNAFKVADGTEPLS
                     VNIGVLSLRMPLDARRGFQDFPYEGDVYLYDAITANPGAAGGAVITAEGKLIGMIGRV
                     IESKSTGTRLNYAVPADLLAAFVRGEEIKPMVAATPGQKADLGIRLFALGGRKAPAYI
                     DRVAPGGPAAASGLRSDDLIVAIGGQVVRDASDFRRLVEGLPVGTEVVIEVKRKNELI
                     SVRLMPVAEK"
     gene            933..2006
                     /locus_tag="VFV87_18185"
     CDS             933..2006
                     /locus_tag="VFV87_18185"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX5105758.1"
                     /translation="MFAVPGIAQESHSPLSRGTLLAPKAFRAAAAKVQPSVVRIEGFG
                     GLTAGAVGGGYQAPGEGPTTGLILSSDGYIVTSTFNFIRKPPVITVVLESGERHIANL
                     LGRDETRRICVLKVGGVSDLPVPKIAPRSELKVGQWAVAIGVGFGGNQPALSTGIISA
                     TSRISTKAVQTDANTSPANYGGPLVDLDGRVIGICVPLSPGGKGDAAGSEWYDSGIGF
                     AASLDGLESILDRLKAGETLKAGFLGVQVEPAGKPAEGKFESGVVVKEVLPDSPAAKA
                     GLEKGDKLVSIAGAKILDPAHLVSLVGRHLSGDNVELDIQRGEESKKLEVTLADAPPP
                     MPMMPPMPAAEGQPKDDEKPPPK"
     gene            2163..>2351
                     /locus_tag="VFV87_18190"
     CDS             2163..>2351
                     /locus_tag="VFV87_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5105759.1"
                     /translation="MPKITKLLAANRSEIAIRVFRAGHELGVRTVAIYSHEDRYALHR
                     FKADEAYLVGQPGEPIKSY"
BASE COUNT          411 a          759 c          785 g          396 t
ORIGIN      
        1 gggctatggc acgggattga ttgtctcgcc gcagggcgac atcctcacgg ctcaaggcgt
       61 gtatctggcg ggcgacagcc tccgcgtgac gctgccggac ggcacaacgc actatgccac
      121 cgtccagcgc cgcagcgacg ccctccaggc ggcggtcatc aagattgaag cggaaacgcc
      181 cgactatttc gaccttcccg cgcagcccgc cgacggaagc gctgcgcggc cggctgccga
      241 gcagggcgac tggatcctgg cggtcagtaa cgctttcaaa gtggccgacg gcacggagcc
      301 actgagcgtc aacatcggcg ttctcagcct gcgaatgccc ctcgacgccc gccgcggctt
      361 ccaggatttc ccttatgaag gcgatgtgta cttgtacgac gcgatcacag ccaaccccgg
      421 tgcggccggc ggcgcggtca tcaccgcgga agggaagctg atcggcatga tcggccgcgt
      481 gatcgagagc aagagcaccg gcacgaggct gaattatgcc gtgccggcgg atttgctggc
      541 ggcgtttgtt cgcggcgagg aaatcaagcc gatggtcgcc gccacgccgg ggcagaaggc
      601 cgaccttggg attcgcctgt tcgctctcgg cggacgcaaa gcgccggcgt acatcgaccg
      661 cgtcgccccc ggcggtcccg cagccgcgtc gggactgcgc agcgacgacc tgattgtggc
      721 gatcggcggc caggtcgtgc gcgacgccag cgacttccgg cggctggtcg aggggctgcc
      781 ggtggggacc gaagttgtca tcgaagtgaa acggaagaat gagttgatca gcgtgcggct
      841 gatgccggtg gcggagaagt agcgggcagg agcaggtaaa agattgtggg tgaaatatga
      901 gtgcaatggg attagctgtg cttgctggac tcgtgtttgc tgtgccaggc atcgcgcagg
      961 aatcgcattc gccactctcc cgcggcacgc tcctcgcccc caaggcgttt cgcgcggcgg
     1021 cggccaaggt gcagccgtcg gtggtgcgga tcgagggctt cggcggcttg acggccggcg
     1081 cggtcggcgg cgggtatcaa gcgccgggcg aagggccgac gacggggctg atcctttcct
     1141 ccgacggcta catcgtcacg agcacgttca acttcattcg caagccgccg gtcatcacgg
     1201 tcgtcctcga gagcggcgag aggcacattg cgaacttgct cggtcgcgac gaaacgcgcc
     1261 gcatttgcgt gctcaaggtc ggtggcgtca gcgatctgcc ggtgccgaag attgctccgc
     1321 gaagtgagct gaaggtcggc cagtgggccg tcgccattgg tgtcggcttt ggcggcaatc
     1381 agccggcgct ttccaccggc attatctccg ccaccagtcg gatcagcacc aaggcggttc
     1441 agaccgatgc caatacgagc cccgcgaatt acggcggacc gctcgtcgat ctggacggcc
     1501 gcgtgatcgg catttgcgtt ccgcttagcc ccggcggcaa gggagacgct gccggctccg
     1561 aatggtacga cagcggtatc ggcttcgccg cttcgctgga cgggctggaa agcattctcg
     1621 accgcctcaa agcgggcgag acgctcaaag cgggtttcct tggcgtgcag gtcgagcctg
     1681 ccggcaagcc cgcggaaggc aagtttgaat ctggcgtcgt tgtgaaagag gtcctgcccg
     1741 attcgcccgc cgcgaaggcc ggcttggaaa agggcgacaa gctcgtttcg atcgccggcg
     1801 ccaagatcct cgacccggcg cacctggtca gcctcgtcgg ccgacacctg tcgggtgaca
     1861 acgtcgagct ggacattcag cggggcgaag aatccaaaaa gctggaagtc acgctggccg
     1921 acgctccgcc gccgatgccc atgatgccgc ccatgccggc ggctgagggc cagccgaaag
     1981 acgatgagaa gccgccgccg aaatagtcgt cgttcgctcc gcgatcgaac gcgccctttt
     2041 gcggagcaaa aggcgacatt ccgcccctga cagtccgccg cctgctgcgg taaactcggc
     2101 ttgcctctcc ccgatgcgcc gctcgctgcc ggcattgttt ctgcccgttc gtgctgcttc
     2161 ccatgccgaa aatcaccaag ctgctggccg ccaaccgcag cgagatcgcc atccgcgttt
     2221 tccgcgccgg ccacgagctc ggcgtgcgga ccgtcgccat ctactcgcac gaagaccgct
     2281 acgccctgca ccgcttcaag gccgacgagg cctatctcgt cggccagccg ggcgagccga
     2341 tcaagtcgta c
//
LOCUS       DASUGB010000961         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_803257, whole genome shotgun sequence.
ACCESSION   DASUGB010000961 DASUGB010000000
VERSION     DASUGB010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803257"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1182)
                     /locus_tag="VFV87_18195"
     CDS             complement(232..1182)
                     /locus_tag="VFV87_18195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5105760.1"
                     /translation="MPLPLVSVVIPTYNYGHLVGEAVQSVLAQNYSMVEIIVVDDGST
                     DDTREQLARFEGRIRYHYQPNAGLSAARNTGIGLAKGQLIALLDSDDAFHPQKLAIQE
                     TYLAANPDVGLVATTSFSGEPIRWQEIPAGDVPDRRVRLEDVVLKSRFAPSSVLVRRE
                     CLDWAGPFDPELKSVEDREMWIRIATHDRMALIDLPLTWYRQTPGSMSRNAAKMEDFE
                     SLVLDRAFAMSEVRSNWTLRRKAKALAALAASRRYQGAMQFGTAFRRLWSSFIWWPLP
                     FGLAESVPLLGRVRLAVAMTRQLIGSYTRSAVARPKPAAA"
     gene            complement(1163..>2288)
                     /locus_tag="VFV87_18200"
     CDS             complement(1163..>2288)
                     /locus_tag="VFV87_18200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5105761.1"
                     /translation="ARNLALGLAGAGHHMSIVTPGGSYRVSDGSVSVQQVRIHHLPLV
                     DRVFPGAGACWRIARAMRRVVRDEGIHVVEFPNWEGLGLLFQSLTRTPVVVRLHTSSR
                     ETQRIDELTSTRRLEWDVRRERWQCRRADALVTHSRAHRRTMSEELEIPEERIELIPH
                     GVLVNPHFQRPRRAKGPPMIVYLGRMEKRKGTLDLLKAVPTVLAKHPQAKFIFIGGDR
                     PHCPAGRTHAQYLAEKFPAAVQQQVTLAGRLPQDEVDRWLQTADIFVAPSLYESFGLI
                     FPEAMRWGTPVIGTRVGGIPEIITDNETGLLVDPQSPEQLASTIVRLLENRELRERLG
                     SAGRRHVETHFTVERMARQTAELYREIIVKRSAKRALATR"
BASE COUNT          452 a          730 c          664 g          442 t
ORIGIN      
        1 ccaagccccc agccacaccg ccgccagccc caccggcgct agccacgccc accgcgggcc
       61 aaaggccacc agcgtgccca gcccatagcc atccgccagc caaatcgcgg cccaagccgc
      121 gcaagctgcg gccataggaa ggacggctgc tagtgacgct aatcgacgcg gccggcttcg
      181 ctcgttggct attcgctgag tcaaacatta cctgtgaagg tggcgtgacg atcaagccgc
      241 agcgggcttc ggccgagcaa cggcacttct agtgtaggag ccgattaact gtcgggtcat
      301 cgcaacggcg agtctgacgc gtcccagcag cggcacgctt tccgccaagc cgaaaggcag
      361 tggccaccag ataaatgagc tccaaagtcg gcgaaacgcc gttccgaatt gcattgcccc
      421 ttgataccgc ctggaagcag ccaatgctgc cagtgccttc gccttgcgtc tcagcgtcca
      481 gttgcttcgc acctcactca tcgcgaaagc gcggtccaga accagcgact cgaaatcctc
      541 catcttggct gcgttgcgcg acatgctgcc gggagtctgg cgataccatg tcagcggcag
      601 gtcgatcagc gccatgcggt cgtgggttgc aatccgaatc cacatttcgc ggtcttcaac
      661 cgatttcagc tcaggatcga acggtcccgc ccagtcaagg cattcacgac gcaccaacac
      721 gctgctgggg gcaaagcgac tcttgaggac gacatcttcc aatctgacgc gccgatctgg
      781 aacgtcacca gcaggaattt cctgccaccg gatcggttcg ccgctgaagg aggtcgtcgc
      841 caccaacccc acatcgggat tggctgccaa gtacgtctcc tggatcgcca gcttttgcgg
      901 atgaaacgcg tcgtccgaat ctagcaatgc gatcagttgg cccttggcga ggccaattcc
      961 cgtgttccgg gccgccgaca ggccggcatt cggctgatag tgataccgaa ttcggccttc
     1021 aaacctggca agctgctcac gcgtatcgtc ggtgctaccg tcatcgacga cgatgatctc
     1081 gaccatcgaa tagttctgcg cgagcacgct ctggaccgcc tcccccacaa ggtggccata
     1141 gttgtaggtt ggaatgacga cgctaacgag tggcaagggc acgtttagcg ctccgtttga
     1201 caatgatttc gcgatacagc tcggcggttt gccgggccat gcgctcgacg gtgaaatgcg
     1261 tctcgacgtg tcgacggccg gcggatccca agcgctcgcg caactcccga ttttcgagca
     1321 gccggacgat ggtactggcg agttgctctg gcgattgggg atcgacgagc agacccgttt
     1381 cgttgtcggt gatgatctcc ggaattcctc cgacgcgcgt cccgatcacc ggcgtgcccc
     1441 accgcatcgc ttccggaaag atcaagccga acgattcgta aagcgacggg gcgacgaaaa
     1501 tatctgccgt ttgcagccag cggtccacct cgtcctgggg caatcgcccg gcaagcgtca
     1561 cctgctgttg cacagctgcc gggaattttt cagctagata ctgcgcatgt gttcgtccgg
     1621 cagggcagtg gggccgatcg ccgccgatga agatgaactt tgcctgcggg tgttttgcca
     1681 agaccgtagg cacggccttc agcaggtcga gggttccttt gcgcttctcc atgcgtccga
     1741 gatagacgat catgggcggg cctttggctc gccgcggtcg ttggaagtgg ggattaacca
     1801 gcacgccgtg tgggatgagc tcgattcgct cttcgggaat ctccaactct tcagacatcg
     1861 tccgtcggtg cgcccgggag tgcgtcacca gggcatcggc ccggcggcac tgccaacgtt
     1921 cgcggcgcac atcccattcc aagcgacgcg tggatgtaag ttcgtcaatt cgctgcgttt
     1981 cccgagacga cgtatgaaga cggacaacta ccggtgtacg tgtaaggctc tgaaatagaa
     2041 gtccaagacc ctcccaattt gggaactcca cgacatggat tccttcgtct cgcacgaccc
     2101 gccgcatcgc ccgtgcaatg cgccagcagg cccccgctcc cgggaagact cggtccacga
     2161 gcgggagatg gtgaatacgt acttgctgaa cgctaacgct gccatcactc accctgtacg
     2221 atccgcctgg cgtcacgatt gacatgtgat gccctgcgcc ggcaagcccg agagcaaggt
     2281 ttctcgcg
//
LOCUS       DASUGB010000962         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1081337, whole genome shotgun sequence.
ACCESSION   DASUGB010000962 DASUGB010000000
VERSION     DASUGB010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081337"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFV87_18205"
     CDS             complement(<1..715)
                     /locus_tag="VFV87_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105762.1"
                     /translation="MNTALDRWPAIVGLAAIFVAAPLQAPASGQDAAEPPAVATPAKP
                     ADVSPTAADELALSQSRLADKYAKLEQLMLKMAELEGASNPKRSALLTRAVEQSKERL
                     TRTQLETLVKLLGQKQLKRAVDGQTAVQTDLKALLELLMSEDRSDRLKSEQQRIREYI
                     KEVERLIRLERSIQGQTEGGSDEKRLAKEQERVAGQTGDLARKIKENEEGAPSDAQQN
                     EGQPKEGEANEGKPNEGKSN"
     gene            complement(724..>3155)
                     /locus_tag="VFV87_18210"
     CDS             complement(724..>3155)
                     /locus_tag="VFV87_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105763.1"
                     /translation="CMATTLPATALTPYPRSLLAGLRWRIRLYVWLEGLAVAVIWLGL
                     MFWLSLGLDYLPVVVGASEMPAAARGVLLAATGIVLAWLFYQFVLRRTFVRLADRSMA
                     LLLERKYTGFHDSLVTAVEMSQVPAHASHFSRDLLAKTADEAFDGAAEVRYRRVINYG
                     RLNLRLLSAVLVAGTVAAFWVTDRQAFATATSRLYLLSDQPWPRSAHIEVEGIEVLRA
                     AVPGQEAPPVTLTFGPERILKVAKGSNVRLKVRAAMAPAAKVVPQQCTIYYHEVQAED
                     GSRGKRGSVPMSSFRDAEGFRHFWFENKPFKGVLSTIVFDVVGYDHRASGYRLEVVES
                     PAVVETLVDLEYPPYMVDEATSNFLPVTGQRYLPAGTFVPLGTKVSLHFRSSKPLVRA
                     EIVATDTLARTVIDVDATGASDDRFSYTIPALKNSLTLEISLQDRDGVATERPYRVFL
                     TGVEDQPPQIDARLRGIGSAVTPDVAIPVRGKVSDDYAVAKAWFDIQVNDSGDPQKRE
                     FALGKAGAVDQTVDFRDERSQPGGLEIKPGDKLFVALKASDKYDLAPEPHVGSGDRYE
                     LDVVTPEQLLAQLEVREVGLRRRFELIIDEMTQMRDSLLRVKASLSPGAAASAEPEDL
                     RDDEGGQPLTPQKKAERVASLRLLRAQRAVQQSLKSVEEVRGVAAGFLDIRDELINNR
                     VDTEDRKNRLKEQIADPLNRTCADEFPLLDQRLAAIETTLRETASAQAADIMAAEKSA
                     AEKGAPLADESIEQANVVLSQLEGVLAKMQDLETYNELLEIVRDLLNDQGKLIDQTQL
                     ERKRQTLEELK"
BASE COUNT          502 a         1088 c          961 g          604 t
ORIGIN      
        1 catttgactt gccttcgttc ggcttgcctt cgttggcctc gccctccttt ggctggcctt
       61 cgttttgttg cgcgtcggac ggagcgcctt cttcgttctc tttgattttg cgggccaggt
      121 ctcccgtctg tccggcgacg cgctcctgct ccttggccag ccgcttctcg tcgcttcccc
      181 cttcggtctg tccctggatc gagcgctcca gccggatcag ccgctcgacc tctttaatgt
      241 attcgcgaat ccgctgctgc tcgctcttga ggcggtccga gcggtcctcg ctcatcagca
      301 gttcgaggag ggccttgaga tcggtctgca cagcggtttg gccatcaacg gcccgcttga
      361 gctgcttctg gcccaataat ttgacgagcg tttccagctg cgtccgggtc agccgctctt
      421 tcgattgctc gacagcccgc gtgagcagcg ccgaccgctt gggattgctg gcaccttcca
      481 gctcggccat tttgagcatg agctgctcga gtttggcata cttgtcggcc agacggcttt
      541 gcgagagggc cagctcatcg gccgcggtag gcgagacatc ggccggcttg gccggcgttg
      601 ccaccgcggg cggctccgcg gcatcctggc ccgaggcggg ggcttgaagc ggagcggcga
      661 cgaaaattgc agccaagccg actattgccg gccagcggtc caatgcagtg ttcatgggcg
      721 cctctacttc agctcctcca gcgtttgccg cttcctctca agctgcgtct ggtcgatcag
      781 cttcccttga tcattgagca aatcccggac gatttccagc aattcgttgt aagtttccag
      841 gtcctgcatc ttggccagca ctccctcaag ctgcgacagc acgacgttgg cctgctcgat
      901 cgactcgtcg gccagcgggg cccccttctc ggccgccgac ttctccgccg ccatgatgtc
      961 ggccgcctgg gccgaggccg tctcgcggag cgtcgtttcg atcgccgcca gccgctggtc
     1021 caggagggga aactcgtcgg cacacgtccg gttaagcggg tcggcgatct gctccttgag
     1081 ccggttcttg cggtcctcgg tatcgacccg gttgttgatc agctcgtcgc ggatgtcgag
     1141 aaacccggcg gccacgcccc gcacttcctc gaccgacttg aggctctgct gcacggcccg
     1201 ctgcgcccgc aggagccgca aactagccac ccgctccgcc ttcttttgcg gcgtcagcgg
     1261 ctggcccccc tcgtcgtcgc gcaggtcctc gggctcggcg ctcgcagccg cccccggcga
     1321 aaggctggcc ttcacccgca gcagcgaatc gcgcatctgg gtcatttcgt cgatgatcaa
     1381 ctcgaatcgc cgccgcaagc cgacctcccg cacttcgagc tgcgccaata actgctcggg
     1441 cgtgaccacg tccagttcgt atcggtcgcc cgagccgacg tgcggctctg gggccaggtc
     1501 gtacttgtcg ctcgctttga gcgcgacaaa aagcttgtca cccggcttga tctccaggcc
     1561 ccccggctgg ctgcgctcgt cgcggaaatc gacagtctgg tccactgcgc cggccttccc
     1621 cagcgcaaac tcccgcttct ggggatcgcc gctgtcgttc acctggatgt cgaaccaggc
     1681 tttggccacg gcgtagtcgt cgctcacctt gccccgcacg ggaattgcca cgtcgggcgt
     1741 caccgccgaa ccgatgcctc gcaggcgggc gtcgatctgc ggcggctggt cctcgacgcc
     1801 agtcaaaaac acgcggtacg gccgctcggt ggcgacgccg tcccggtcct gcagcgagat
     1861 ttccagcgtc aggctgtttt taagcgcggg aatcgtatag ctgaagcgat cgtccgacgc
     1921 gccggtggcg tcaacgtcga tcaccgttcg cgcgagcgta tcggtcgcca cgatttcggc
     1981 ccgcaccagc ggtttgctgg accggaaatg gaggctcacc ttggtcccca gcggcacgaa
     2041 cgtacccgcg ggcaaataac gctgccccgt gaccggcagg aagttcgacg tcgcctcatc
     2101 gaccatgtac ggcgggtact ccaggtccac caaagtctcg acgacggccg ggctctcgac
     2161 cacttccagc cgatagccgc ttgcccggtg gtcgtagccg accacgtcga acacgatagt
     2221 cgagagcact cctttgaatg gcttgttctc gaaccagaaa tgacggaagc cttcggcgtc
     2281 gcggaagctg ctcatgggga cgcttccccg cttgccccgc gatccgtcct cggcttgcac
     2341 ctcgtggtaa taaatcgtgc actgctgggg aacaaccttg gcagcgggcg ccatggcggc
     2401 ccgcaccttg agccggacgt tggacccctt ggccactttg agtatgcgct ccgggccgaa
     2461 ggtgagcgtg accggcggcg cctcctgacc gggaacagca gcccgcagca cctcgatccc
     2521 ctccacttca atgtgggcgc tgcgcggcca cggctggtcg ctgagcaagt acagcctgct
     2581 ggtggccgtg gcgaacgcct ggcggtccgt cacccaaaat gccgccaccg ttccggcgac
     2641 cagcacggcc gagagcagac gcaggttcaa gcggccataa ttgatcacgc ggcgatacct
     2701 gacctctgcc gccccgtcga aggcctcatc cgcggtcttg gcgagcaaat ctcggctgaa
     2761 gtgcgatgcg tgggcgggaa cctgggacat ctcgacggcg gtgaccagac tgtcgtgaaa
     2821 accggtgtat ttccgctcca ggagcagggc catgctccgg tcggccaggc gcacgaacgt
     2881 ccgccgcagg acgaactgat aaaacagcca ggcgagcacg ataccggtgg ccgccagaag
     2941 cacgccgcgg gcggccgccg gcatttcgct tgcccccacg accaccggca agtagtccag
     3001 ccctaggctg agccagaaca tcagccccag ccagatgacc gccacggcca gcccctccag
     3061 ccagacatag aggcgaatcc gccaccgcag ccccgccaaa agcgagcggg gataaggagt
     3121 taaagcggtc gcaggcaagg tggtggccat gcagt
//
LOCUS       DASUGB010000963         4842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_943177, whole genome shotgun sequence.
ACCESSION   DASUGB010000963 DASUGB010000000
VERSION     DASUGB010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943177"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..882
                     /locus_tag="VFV87_18215"
     CDS             88..882
                     /locus_tag="VFV87_18215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5105764.1"
                     /translation="MPLVQLADISLHVIDRGSGTPLVLVHGFPLDHTMWRGQIDGLAD
                     VCRVIAPDLRGFGRSGVTAGTVTMAQFADDLAALLDTLAVMEPVVLCGLSMGGYIAWQ
                     FALRHRRRLSKLILCDTRAIADTPDGAAGRIKTAERVLAEGSSVVAEPMLGKFFAPAT
                     VSQRPELIEETRQVILRTAPEGIAAALRGMAERPDVSGRLADFDTPALVVGGEHDVIS
                     TPSEMHDFTGKMPQARFVKISDAGHMAPLEQPAAVNQVLRAFLDFV"
     gene            907..1110
                     /locus_tag="VFV87_18220"
     CDS             907..1110
                     /locus_tag="VFV87_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105765.1"
                     /translation="MLMHLESAGPTTIGFRLIVVAGGMWATGFGAAEYGWISWLMTPI
                     AIGVFGSGLCDLAASFRSLEGQK"
     gene            1265..2401
                     /locus_tag="VFV87_18225"
     CDS             1265..2401
                     /locus_tag="VFV87_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5105766.1"
                     /translation="MRWWRPAINRTLRRDQKIVELDIGGVQHVQRALAEGAGVLVTPN
                     HSFHYDSYVLIEASHRVGTPFHFLTAWQVFAMSRWYEQLVLQWHGCFSVNREASDLAA
                     FKTAVDILREKREPLVIFPEGDIYHNNDRVTPFRDGAAAIAISAAKKAERPIVCVPCA
                     LKCFYLDDPTDVLKELMSRLEQALHWRPRPDLPLPERIYRFAEGSLALKEYEYLGQPQ
                     SGSVPERATRLADQVLSRLELRHGLKNKGGIIPERVKEVRRAVIKAIEQDGATSEGRE
                     RLQADMEDLFFVIQLFSYPGDYVSQRPSIERIAETLDKFEEDVLQVDYPAIRGTRKAV
                     LRFGEPIPVPKEREAKNSVEKWTDLVESRVQGLLDEINCNVSGE"
     gene            2465..3253
                     /locus_tag="VFV87_18230"
     CDS             2465..3253
                     /locus_tag="VFV87_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5105767.1"
                     /translation="MLDLIDVSKSFGDLQVVKPTTLRLEPGRTTVLIGPSGCGKSTLL
                     RLMIGLIDPDQGQVLFDGQPVTPANVMEVRRKIGYVIQDGGLFPHLSAEGNVGLLARH
                     LGWESSRVSSRIEELADLTRLPHEALSRYPAQLSGGQRQRLGIMRALMLDPEVILLDE
                     PMGALDPLVRFDLQEDLRKIFTSLKKTVAMVTHDMGEAGFFGDRVILLGSGRIVQEGT
                     LDDLIRDPADDFVRRFINAQRLPGVNEEGSAFSRQETGVRSETE"
     gene            3349..4803
                     /locus_tag="VFV87_18235"
     CDS             3349..4803
                     /locus_tag="VFV87_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5105768.1"
                     /translation="MRIGSKNYTENVILGEMLTLLASAAGARAEHKAELGGTQIAFES
                     LRSGKIDAYPEYTGTLVMEILLHEAPKSDADIEAALARQGIKISRRLGFSNAYAIGLK
                     RTLAAELELTKISDLAERQSDPAVARLKLGFSDEFMNRGDGWPGLSYAYGLPDRARGL
                     DHNLAYRGIRSGTIHITDVYTTDPEIKAYDLTTLDDDRGYFPQYHCVLLYRADLAERA
                     PRVVESWLRLEGVIDEPTMIALNGQARIDRASEGRVAAEFLNEKLQANVPVPPDDRWR
                     RMLRNLAINTRQHLLLVSSSLLAAIVIAVPLGILAYRVPASRHVILGSVGVLQTLPSM
                     AVLMFLLPLLGLGAWPTIVALFLYSLLPIVRNTFNGLQEIPGNLRESATVLGLEPRAR
                     LWLVELPMASRSILSGIKTAAVINVGTATIGGLIGAGGYGQPIITGTRLNDITIIMQG
                     AIPAALMALAAQWLFDFAERLVVPKGLRIAAGKA"
BASE COUNT          855 a         1484 c         1609 g          894 t
ORIGIN      
        1 tagcagtaca atgtcccgtg cggtcgccgc gcgtgcggtt ggccgagaag atcgctgcgt
       61 gagaatgggc gccgcggagg cgaagacatg ccgcttgttc aacttgctga catttcgctc
      121 cacgtgatcg atcgcggaag cggcacgccg ctggtgctgg tgcatgggtt tccgctggat
      181 catacgatgt ggcgggggca gatcgacgga ctggccgacg tgtgccgggt gatcgcaccc
      241 gatttgcggg gctttggaag gagcggcgtc acggcgggga cggtcacgat ggcccagttt
      301 gcggacgatc tggcggccct gctggacacg ctcgccgtta tggagcccgt ggtgctgtgc
      361 ggactgtcga tgggcggcta catcgcctgg cagttcgcgc tccgccatcg ccgccggctg
      421 agcaaactga tcctgtgcga cacgcgggct attgccgaca cgcccgatgg agcggcggga
      481 agaatcaaga ctgccgagcg ggtgctggcg gaagggtcga gcgtcgtcgc cgagccgatg
      541 ctgggcaaat tcttcgcgcc ggcgaccgtc agccaacggc cggaattgat cgaggaaact
      601 cggcaagtga ttctgcgtac cgcgccggag ggcatcgccg ccgccctgcg cgggatggcg
      661 gagcggccgg acgtgagcgg gcggctggcc gatttcgata cgccggcact ggtggtcggc
      721 ggcgagcacg acgtgatttc aacaccgagc gaaatgcacg atttcaccgg caagatgcca
      781 caagcccgct ttgtaaagat cagcgacgcc ggtcacatgg cgccgctgga gcagcccgcg
      841 gcagtgaatc aagtgctgcg ggcttttttg gactttgttt aatgcgtagc cgaagacgca
      901 gcgtcgatgt taatgcactt ggaatccgcc ggaccgacca ccatcggctt tcgcctgatt
      961 gtggtagccg gcggaatgtg ggccacgggg tttggcgcgg ctgagtacgg ctggatatcg
     1021 tggctgatga cgcccattgc aatcggcgtt ttcgggtcgg gactttgcga tttggcggcc
     1081 agtttccgct cgctggaagg gcagaaatga ttgccaacga gcccggcagt ttctacaaca
     1141 ttcagcagac tcttgcgacg tttgtattcg ccgaaagcga gttcgtttcg ccgggctgct
     1201 agtgaaccgt caaccctttc aaactccgcc gcgggtctgg tcgccgaaga tgacgccttg
     1261 gctggtgcgt tggtggcgac cggcgatcaa ccgcacgctg cgccgcgacc agaagatcgt
     1321 ggagctggat atcggcggcg ttcagcacgt gcagcgggcg ctcgccgagg gcgcgggcgt
     1381 gctcgtcacc cccaaccatt cgtttcacta cgattcgtac gtcctgatcg aggcttcgca
     1441 ccgcgtcggc actccgttcc acttcctcac ggcctggcag gtctttgcca tgagccgctg
     1501 gtacgagcag ctcgtgttgc agtggcacgg ctgcttcagc gtcaaccgcg aggcgagcga
     1561 cctggcggcc ttcaagacgg ccgtcgatat cctccgcgag aagcgcgagc cgctggtgat
     1621 ctttccagaa ggggatatct accacaacaa cgaccgcgtg acccccttcc gcgacggagc
     1681 ggcggcgatc gccatatcgg ctgcgaagaa ggccgagcgg ccgatcgtgt gcgtcccctg
     1741 cgcgctgaag tgcttctatc tggatgatcc gacggacgtc ttgaaggagc tcatgtcccg
     1801 cctggagcag gctctgcact ggcggccgcg gcccgatctg ccactgccgg agcggattta
     1861 tcgctttgcc gaaggatcgc tggcgcttaa ggaatatgaa tacctcggcc agccgcagag
     1921 cggcagcgtg cccgagcggg cgacgcgctt ggccgatcag gtgctctctc gcctggaact
     1981 gcggcacggc ctgaagaaca agggcgggat catcccggag cgcgtaaaag aagtccggcg
     2041 cgctgtaatc aaggcaattg agcaggacgg cgcaaccagc gagggccgag agcgtctgca
     2101 ggccgacatg gaggacctgt tttttgtcat ccagctcttc agctatcctg gcgactacgt
     2161 ttcccagcgg ccttcgatcg agcggatcgc cgagacactc gacaagtttg aggaagatgt
     2221 gctccaggtg gactatcccg ccatccgcgg cacccgcaag gcagtcctgc gcttcggcga
     2281 gccgattccc gtccccaagg agcgggaagc caagaacagc gttgagaagt ggaccgacct
     2341 ggtcgaaagc cgcgtgcagg gattgctgga cgaaataaat tgcaatgtga gtggtgagta
     2401 ggtgagtggt gtatccgtga tacaactcac tactcacccc tctcaccact cacctgcctc
     2461 ccccatgctc gacctcatcg acgtctccaa gtcgttcggc gacttgcagg tggtcaagcc
     2521 cacgacgctg cgcctggagc cgggccggac gaccgtgctg attggcccca gcgggtgcgg
     2581 gaaatcgacg ctgctgaggc tgatgatcgg cctgatcgat ccggatcagg ggcaggtcct
     2641 cttcgacggg cagccggtga cgccggccaa cgtgatggag gtgcggcgga agatcggcta
     2701 tgtgatccag gacggcgggc tctttccgca tctgtctgcc gaggggaacg tgggccttct
     2761 ggcgaggcat ttgggctggg aatcatcgcg cgtgtcttct cggatcgaag agctggccga
     2821 tctgacgcgc ctgccgcacg aagccctcag ccgttatccg gcccagcttt ccggcggcca
     2881 gcggcagcgg ctgggaatca tgcgggcgct gatgctcgat ccggaagtga tcctgctcga
     2941 cgagccaatg ggcgccctgg atccgctcgt gcggttcgac ttgcaggaag acctccgcaa
     3001 gatctttaca tcactcaaga agacagtggc gatggtcacg cacgacatgg gcgaagccgg
     3061 ctttttcggc gaccgtgtaa tccttctcgg cagcgggcgg atcgtccagg aagggacgct
     3121 cgacgatctg atccgcgatc ctgccgatga cttcgtccgc cgcttcatca atgcccagcg
     3181 gctgccgggc gtaaacgaag aaggatcggc gttcagccgt caggagacag gggtcaggag
     3241 cgagacggaa tgaatatgcg cgatgctggg actgccgccc ttctcgcagc gctagtcgct
     3301 gtcgccacgg ttagctcgcg cgcggcttgc gcgcagcagc ccccgcgcgt gcggatcggc
     3361 tcgaagaact acaccgagaa cgtgatcctc ggcgagatgc tgacgttgct cgcctccgcc
     3421 gccggcgccc gcgccgagca caaggcggag ctgggcggca cgcaaatcgc cttcgaatcg
     3481 ctccggtccg gcaagatcga tgcgtacccc gaatacaccg gcacgctggt gatggagatc
     3541 cttctgcacg aggccccgaa gagcgacgcc gacattgagg cggcgctcgc ccgccaggga
     3601 atcaagatca gccggcgact ggggttcagc aatgcctatg cgattggctt gaagcggacg
     3661 ctcgctgccg agctagagct tacaaagatt tcggacctgg cggagcggca aagcgatccg
     3721 gctgtggccc ggctgaagct gggtttcagc gacgagttca tgaaccgcgg cgacggctgg
     3781 ccgggattgt cgtacgccta cggactgccc gaccgggccc gcggactcga tcacaacctg
     3841 gcctaccgcg gcatccgcag cgggacgatt cacatcacgg atgtctacac gaccgatccg
     3901 gaaatcaaag cttacgatct gacgacgctc gacgatgacc gcggctattt cccgcagtac
     3961 cactgcgtcc tgctctatcg ggccgatttg gccgagcgag cgccgcgagt cgttgagagc
     4021 tggctgcggc tggagggagt gatcgacgag ccaacgatga tcgccctcaa tggccaggcg
     4081 cggatcgacc gtgccagcga agggcgcgtc gcagccgagt ttctgaacga gaagctgcag
     4141 gcgaatgtgc cggttccgcc cgacgaccgc tggcggcgaa tgcttcgcaa cctggcgatc
     4201 aacacgcggc agcatctgct gctggtttca tcgtcgctcc tggccgcgat cgtcattgcc
     4261 gtgccactgg gcattctggc gtatcgcgtg cccgcctccc gccatgtaat cctgggcagc
     4321 gtcggcgttc tgcagacgtt gccgtcgatg gcggtcctga tgttcctgct gccgctgctg
     4381 ggccttgggg cctggccgac gatcgtcgcc ctgttcctct acagcctgtt gccaatcgtg
     4441 cgcaacacgt tcaacggctt gcaggagatt cccggcaacc tccgcgaatc ggcaaccgtc
     4501 ctgggcctgg agccgcgggc ccgcctgtgg ctggtcgaac taccgatggc ttcgcgctcg
     4561 attctctcgg ggatcaagac ggccgcggtg atcaacgtcg gcacggcgac gatcggcggc
     4621 ctgatcggcg cgggcggcta tgggcagccg atcatcaccg gcacccgcct gaacgacatt
     4681 acgatcatca tgcagggggc gattcccgcc gccctgatgg cgctggccgc gcagtggctg
     4741 ttcgactttg cggaacgctt ggtggtgccc aagggactgc gaatcgcggc cggcaaggca
     4801 tgagccgccg gccgctcgac tattgctttt tcctctggtt gg
//
LOCUS       DASUGB010000964         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1638827, whole genome shotgun sequence.
ACCESSION   DASUGB010000964 DASUGB010000000
VERSION     DASUGB010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638827"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..786)
                     /locus_tag="VFV87_18240"
     CDS             complement(160..786)
                     /locus_tag="VFV87_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105769.1"
                     /translation="MIRTLNRPILGALCLLTGSGWASAADRVILGSSGKLNSIVVMQQ
                     PFKPEVLPAADPAASDRLRPIDQVELTLGARQEILPPDLAAERFGPDEALFQPTGSSR
                     LWGDSHYSWDAPALCHRPLYFEEENLERYGRSYGLLQPAVSVAHFSGRVAALPYLMGA
                     YPPHECIYTLGRTPPGSYEPYHWYRPPASARGAVLQAGAVTGLIFLVP"
     gene            1587..1778
                     /locus_tag="VFV87_18245"
     CDS             1587..1778
                     /locus_tag="VFV87_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105770.1"
                     /translation="MQSESVALQPAAEEACWDEMRRRWADGEPLLAEEAFSRWPQLMV
                     EETTAVDVIRRVCTSSAVR"
     gene            complement(2213..3343)
                     /locus_tag="VFV87_18250"
     CDS             complement(2213..3343)
                     /locus_tag="VFV87_18250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX5105771.1"
                     /translation="MSAIADAALEQIVSSSSRPAPPAPVEFRYSQSDSFVAVLYELGA
                     TLLVSTYQANKLLAVRASGQGLSTLVRTFDKPMGLAVDGSRLAVGTRKEVWFLRNAAD
                     IAPQIEPAGMHDACYLPRMSHVTGDIGIHEIAWATPHLQTRSHNGRGEKELWIVSTRF
                     SCLATLDPDYSFVPRWRPPFISAIAAEDRCHLNGLAIANGQPRFVTALGTTDVRDGWR
                     AEKARGGCIIDISGEEFVARGLCMPHSPRWHDGKLWVLDSGTGGVVLVDSATGKRETI
                     TQLPGFTRGLAFAGTYAFVGLSKIRPTSAMDGVPLADRRDELRCGIAVVDLRSGQTSA
                     FLEFQTAVEEIFDVQLLAGSRFPEVVGFQKDALHHTFVIPPN"
     gene            complement(3436..>3619)
                     /locus_tag="VFV87_18255"
     CDS             complement(3436..>3619)
                     /locus_tag="VFV87_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105772.1"
                     /translation="VQLSGAYGASISDSQGTGTIVNDDTGGKGNGKGNAKLSSAAAFD
                     AALTDLLTPGSKKRGR"
BASE COUNT          721 a         1144 c         1101 g          653 t
ORIGIN      
        1 ccggtcctga tcggcgccgt cggctgggcc aaagttggcg tcgatccaca gccattcggt
       61 ttgcatctct atcgggttct gcggagcacg ttccgcctgc cgtggtaacg accacggtgt
      121 gccgtgggac gtcggatacc ggccgctaac taccagcctc tacggcacca ggaagatcag
      181 ccccgtcacc gctccggctt gcagcaccgc cccgcgcgca cttgcgggcg gccggtacca
      241 gtgatacggc tcgtagctac ccggcggcgt gcggcccagc gtgtaaatgc attcgtgggg
      301 aggataggcg cccatcaggt acggcaacgc cgcgacgcgc ccggagaagt gggcgacgga
      361 aacagccggc tgaagcaggc cgtacgaacg tccgtagcgc tccaggttct cttcctcgaa
      421 gtacaacggc cgatggcaca acgcaggcgc gtcccacgaa taatgagaat ctccccacaa
      481 tcggctgctg ccggttggtt ggaacagcgc ctcgtcgggg ccgaatcgtt cagccgccag
      541 gtcgggcggc aagatttcct gtcgcgcccc cagtgtcaac tcgacctgat cgatggggcg
      601 caatcgatcg ctggcagcag ggtccgcagc cggaagtact tcgggtttga acggctgctg
      661 catcacgacg atggaattca gcttgccaga cgagccaagt atcacccgat cggcagcaga
      721 ggcccagccc gatccagtca gcagacacag cgccccgagg atggggcgat taagagtacg
      781 gatcaaggag agagtcacag cagcaccccg aagtggcttc tatccgtagg tcggtcacaa
      841 cgggctctat cggcgcaaaa cctacgaccg gtccattgct acccgaatag aacaggcacg
      901 aatccgatgg aagggtccga cagcgatgcc tttgcgggac gtagtgaagt acgacgtcgt
      961 tatttctcat gcgttgcgaa aggccgctac aaccggcacc atcggcccga ctagccccca
     1021 cgtgttggcg tcataagcta ggtgagacgc attccgtcga atcccgtttc gaagcaatgc
     1081 attccgaatc gctcggatag ctcgcggcac gtggcgacgc cccggacgct gttcccttta
     1141 gcatcgagca gaggagaaaa gatgccgatt ccgaattggc caggtacaac gccgacgatc
     1201 ccctcccacg ccgcttttgg acgggatgcc aacgcgaaag tccattcgcc ggcgtagtcg
     1261 tacatcccgc aggaggcctg tcacactgag caggtacttc acatattttt gctcaatcgc
     1321 ccgttgacca gtgacgggat cgatgccgcc gccagccagc gtcgccccca tgaccgcgag
     1381 atcgtgacaa ttaacgacca ccgaacactg ctggaaatag agctccaacg attcatccat
     1441 gcgctcgcca accatcccga aattccgcat caagcggccg atcgcccggt tccgatgtcc
     1501 cgtcatccgt taccaagccg cgaaggcgct tggtttggcg cgggagtgaa gaggctcaaa
     1561 gaaccatcag ccaagcgaga cacaccatgc aatccgaatc cgtggcactg cagccggcgg
     1621 cggaggaggc ctgttgggac gagatgcggc gccgctgggc cgatggcgag ccgctgctgg
     1681 ccgaagaggc cttttcgcgc tggcctcagc taatggtcga agagacaact gcagtcgatg
     1741 tgatccggcg agtatgtact agctcagcag tacggtaagg ctgcagagtt tgcagactat
     1801 ctccagcgat tccccaccgt ggcgaccgcc cttgtgcgac agctgcattt gcactaagcg
     1861 ctgtcctcga ccatcgcccc gcccgacccg ctggcgttca aggcaatctt gtcgcttccc
     1921 cagccgaccg cgttcaacgc cctgctgcat ggattcgacg gcgaatgccc gtgcggtcgg
     1981 agaaagttca gtcaagcgta gtcaacctgg ccaaccggta ggccgacacc tctgctgcca
     2041 atctgcagag gtgtcatcac cgcccgcagt taggcgaggc gccaagctgc ggcggttggt
     2101 ccaaccgcct gcagtctgat ctacggcgaa ctagcgtcgg tgtgtttgat cgacgtggtt
     2161 ttgtggtgct ggcgctcgtc gttcgttact cgcacagtaa cgcgaaacaa cctcaattgg
     2221 gtggaatgac gaacgtatga tgcagcgcgt ctttctggaa tccgacgacc tcggggaagc
     2281 ggctgccggc caagagctgc acatcaaaga tttcttccac ggcggtctga aactccagga
     2341 aggcactggt ctggccgcta cgaagatcga ccacggcgat tccacatcga agctcatctc
     2401 gtcggtcagc cagaggcacg ccatccatgg cggacgtggg gcggatcttc gagaggccga
     2461 caaaggcgta ggtgccggcg aaggccaaac cgcgggtaaa gccggggagc tgcgtgatcg
     2521 tttcgcgttt gccggtggct gagtcgacga gcactacgcc gccggttccc gagtcgagca
     2581 cccacagctt gccgtcatgc caccttggcg aatggggcat gcacaaacca cgggcgacga
     2641 attcttcgcc ggagatgtcg ataatgcagc cgcccctcgc cttttccgca cgccacccgt
     2701 cgcgaacatc ggttgtgcca agtgcggtaa cgaatcgcgg ctgtccattc gctatcgcca
     2761 ggccattcaa gtggcagcga tcttcggccg cgatcgcgct aatgaacggt gggcgccagc
     2821 gcgggacgaa gctgtagtcg ggatcaagcg tcgccagaca tgagaaccgc gtgctgacga
     2881 tccacagttc cttctcccct cgcccattgt gggagcgggt ttggaggtga ggggttgccc
     2941 aagcaatctc gtgaatgcca atgtcacccg tgacatggct catccgcggc aggtagcaag
     3001 catcgtgcat gcctgccggc tcgatctgcg gcgcaatatc ggccgcgttt cgcagaaacc
     3061 acacttcctt gcgcgtgccg acggccaggc gggatccgtc cacggccagc cccatcggct
     3121 tatcgaacgt gcgaaccaac gtggacaatc cctgtccgct agctcgcacg gcgagcaact
     3181 tattggcctg ataggtgcta accaagagcg tcgcgccgag ttcgtacaag accgcgacga
     3241 agctgtcgct ctgcgaatag cggaattcga ccggcgccgg cggcgcgggc cgcgagctgg
     3301 agctgacaat ctgttcgagt gcggcgtcgg cgatggcgga catgggtact ccaatctgta
     3361 ggccccgtac ggtggccctc ccgggcccac gaggacggcc ccgaagtgcc gccccctgcg
     3421 atgccgcgcg acacgctatc ggccgcgctt ctttgacccc ggtgtcaaca agtcggtcag
     3481 cgcggcatcg aaagccgcgg ccgaggaaag cttcgcattg cccttcccgt tgcctttgcc
     3541 gccggtgtcg tcattgacga tcgttccggt gccctggctg tcgctgatcg aagcgccgta
     3601 ggccccggaa agttgcacg
//
LOCUS       DASUGB010000965         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1081717, whole genome shotgun sequence.
ACCESSION   DASUGB010000965 DASUGB010000000
VERSION     DASUGB010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081717"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1718)
                     /gene="tig"
                     /locus_tag="VFV87_18260"
     CDS             complement(216..1718)
                     /gene="tig"
                     /locus_tag="VFV87_18260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5105773.1"
                     /translation="MSTTEVDDREQTAGEGQGEVQKEKPRLSLEVKVEKPSACQRHVV
                     VSIAKEDVQRYVKEAFDELMPKAEIRGFRPGHAPRKLIERQFKEHVGDQVKGKLLMDS
                     LTQMGDDHEFTAISEPDFDMNSIQLPDDGPMVFEFDIEVRPEFDLPRWKGLKLNKPVH
                     EYSDAEVDERLRLLLAKYGRLEAVERPIQAGDFVTLTLRATSGGEQISQLTEETIEVR
                     PTLSLLDARLEGFDKLLIGKNVGDTVETKITVSEESDSEAYRGKEVDLSMEIVALEER
                     RLPELSQAFLEKIGGFKDEDELRAEVRKELERQLRYYQQKQVREQITAQLTITATWDL
                     PKELLRKQAGREMERAIMELQSAGFPDEAIRAHQNELRQNAHASTARALKEHFILERI
                     AEDEKIEANDDDYDREVELIAEQADESPRKVRARLEKRGLMDTLRNQIVERKAIELIE
                     QNADFIEKPYTPPKETVVAVHYAVAGHAAEAIPNAEHDEGDSPTQPGKTA"
     gene            complement(1873..1945)
                     /locus_tag="VFV87_18265"
     tRNA            complement(1873..1945)
                     /locus_tag="VFV87_18265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1910..1912),aa:Gly,seq:gcc)
     gene            complement(2253..2615)
                     /locus_tag="VFV87_18270"
     CDS             complement(2253..2615)
                     /locus_tag="VFV87_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105774.1"
                     /translation="MKSTKASLILPVLLVFLGIGWLLTELKILPPVNWAWSLGLAAIG
                     VLAFIVSGFDKVSIVIGPFFLVAAGLSLLRQQGWLAEDVELPILVTLAGALLLTARLP
                     AIPLPSWLIQDGGSVQKA"
     gene            complement(2664..>2923)
                     /locus_tag="VFV87_18275"
     CDS             complement(2664..>2923)
                     /locus_tag="VFV87_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5105775.1"
                     /translation="NRSQLARAYFRLAESTRFVGSLQILPFSEAAITRFEQLKALKLG
                     VKSPDLRIAAIALESGDIVVTRNLRDFQLVPGLRVEIWSSP"
BASE COUNT          518 a          882 c          895 g          628 t
ORIGIN      
        1 ggaatatgaa tcgaagatcg ggggaggcag atgcgggttc atcgagtgtc cttacgttgc
       61 gaggtctagg cgcattattc ccctgccagt cgattcgggc cagaccggat aagaatgacc
      121 aatgaccaaa tcccaatgac caacggaatt tcgcgatccg ttactcctca tttgtcattg
      181 gtgcttggtc attggtcatt tccgaacgag cgggactaag cggtcttccc aggttgcgtg
      241 gggctgtcgc cttcgtcgtg ttcggcgttg ggaatcgctt cggcggcatg cccggcgacg
      301 gcgtagtgca cggcaacgac ggtttccttg ggcggcgtgt agggcttttc gatgaagtcc
      361 gcgttctgct cgatcagctc gatggccttc cgctcgacga tctggttgcg gagcgtgtcc
      421 atcaggcccc gcttttccag ccgggcgcgc accttgcggg gcgattcatc ggcctgctca
      481 gcgatcagct cgacctcgcg gtcgtaatcg tcgtcattcg cctcgatttt ctcgtcctca
      541 gcgatccgct ccaggatgaa atgctccttg agggcccggg ccgtggaggc gtgggcgttc
      601 tggcgcagct cgttctgatg ggcccggatg gcttcgtccg ggaagccggc cgactgcagc
      661 tccatgatgg ctcgctccat ctcgcggccg gcctgcttgc gcaacagctc cttgggcagg
      721 tcccaggtgg cagtgatcgt gagctgcgca gtgatctgct cgcggacctg cttctgctgg
      781 tagtagcgaa gctgccgttc cagctccttg cggacctcgg cccgcaactc gtcttcgtcc
      841 ttgaagccgc cgatcttttc caggaaggcc tgcgagagct cggggagccg gcgttcctcg
      901 agggcgacga tctccatcga cagatcgacc tccttgccgc gataggcttc gctatccgat
      961 tcctccgaca ccgtgatctt tgtttcgacc gtgtcgccga cgttcttccc aataagcagc
     1021 ttgtcgaaac cttccaggcg ggcatcgagc aggctgagcg tggggcggac ttcaatggtt
     1081 tcttcggtga gctgcgagat ctgctcgccc ccggaggtgg cccgcagggt caatgtcacg
     1141 aagtcgccgg cctggatcgg ccgctcgacc gcctccagcc ggccgtattt cgccagcagc
     1201 agccgcaggc gctcatcgac ctcggcatcc gaatactcgt gtaccggctt gttgagcttc
     1261 agtcccttcc aacgcgggag gtcgaactcg ggccggactt cgatgtcgaa ctcgaagacc
     1321 atcggcccgt cgtcgggcag ttggatggag ttcatgtcga aatcgggctc gctgatagcg
     1381 gtgaactcgt gatcgtcgcc catttgggtc aggctgtcca tcagcagctt gcccttgacc
     1441 tggtcgccga cgtgttcctt gaactgccgc tcgatcagct tgcgcggcgc gtgccccggc
     1501 cggaagccgc ggatctcggc tttgggcatg agttcgtcga aggcttcctt gacgtagcgc
     1561 tggacgtctt ccttggcaat gctgacgacg acgtgccgct ggcaggcgct gggcttttcg
     1621 accttgactt ccaggctcaa gcggggcttt tccttttgta cctcgccttg tccttcgccg
     1681 gcggtctgtt cgcgatcatc gacttcggtg gtactcatgc gttctgattc ctgacgagcg
     1741 atgttggctg attccacaaa gcaaaaaccc gaagcgtcag cacgtgtccc tggcgcttcg
     1801 ggttgctgcg ccgattcgcc gctcggctgg ttgaccagcc tggaggggag cgattcgtct
     1861 gccgcgatca agagcgggtg atgggactcg gacccacgac ctacacgttg gcaacgtgtc
     1921 gctctaccaa ctgagctaca cccgcaaatg tcagaccgtc attttgccaa aaccgctcct
     1981 cttgtaaagc ccgcgccagc gtcagagctt acctgaccgg caggggtcgc aacccgttgc
     2041 tggccaacgt gttggcgcgg acgagcgaag cggcggattc tgccgacaga tgcctgctct
     2101 actatcgacc gttcaggcgc tggaaggttc gtttcaaccc acgccaatcc cggcaattac
     2161 gcaagcgacc gccagccgga taccgggagc tagctgtggg tcgatggctg aaatgattgc
     2221 tcccatgatc cagttactgc tagggaacat tctcaggctt tttgaaccga cccgccgtct
     2281 tggattagcc aacttggcag gggaatggcc ggaagacggg cggtgaggag caatgcgccg
     2341 gccagcgtta cgaggatcgg caattcaacg tcctcagcga gccatccttg ttgacgcagg
     2401 agcgaaaggc cggcggccac gagaaagaac gggccgatca cgatactgac cttgtcgaag
     2461 ccgctgacga taaacgccaa cacgccgatc gccgccaggc ccagcgacca ggcccagttc
     2521 accggcggca agattttcag ttcggtgagt agccagccga tgccgaggaa gacgagcaac
     2581 accggcagga tcagcgaagc ctttgtcgat ttcacgttga ggtctccacg gttgggcttg
     2641 gaccatcttt gcaagggagc aaattacgga ctcgaccaga tctcgaccct caatccggga
     2701 acaagctgga agtcccggag attgcgggtc accacgatgt cgccgctctc cagcgcgatg
     2761 gcggcaattc gcaggtcggg cgatttgacc ccgagtttca gcgccttgag ttgttcaaag
     2821 cgtgtaatgg ctgcttcgga gaagggcaga atctgcaacg agccgacaaa ccgcgtgctt
     2881 tccgcgagtc gaaagtacgc gcgagcgagc tgcgacctgt tcc
//
LOCUS       DASUGB010000966         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1081820, whole genome shotgun sequence.
ACCESSION   DASUGB010000966 DASUGB010000000
VERSION     DASUGB010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081820"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VFV87_18280"
     CDS             complement(<1..819)
                     /locus_tag="VFV87_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5105776.1"
                     /translation="MPARDPILAGPPGTSHVPQPYAGPPKDEVLALRAQYLTPGLITY
                     YREPLMLVEGHMQYVWDERGKQYLDAFAGIVTVSVGHCHPEIVERVREQVGRLQHTTT
                     IYLHPTIGQFAQKLADHMPAGSGLSVSYFTNSGSEANEVAILSAREFTGNQEVISLRN
                     GYHGGTQATMGLTGHATWKFRSSPTIGVKNALPGYCYRCPLGLEYPSCNVQCARDVET
                     LIRTETSGEIACFIAEPIQGVGGAVTPPPEYFQIVYDIVRKHGGLCIADEVQTGF"
     gene            1059..>2374
                     /locus_tag="VFV87_18285"
     CDS             1059..>2374
                     /locus_tag="VFV87_18285"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HEX5105777.1"
                     /translation="MSPSSIAHCAAIWLALFGSTYAQSQGPAPGRQPAPVRTSPPAAQ
                     QAEKTPAQRPVQPAAHQEVLPEVAPLPGEQMYYEGEGPVFADDWGTCGPGGCNFCGQR
                     GPGCGPFGQGCLSGVYVRAEYLLWGAKGATLPPLVTSSPPGTAQNVAGILGEPDTVIL
                     FGDETVNNDLRSGGRLVLGWWFDPCRRLGIEGDYFSLDDESTDFRASSTGTTIIGVPF
                     YDVVQGIENVNLVAFPNLIQGTIAADYRTTFQGAGIRAMYNLACGEGGGCSWITGCPV
                     HTGFRFDMLLGYRFVRLDDNFTLVENSVSLSTANPGSFNIRDQFDTENQFHGIDLGTS
                     LSFCKGCFSLEMLSKIALGNTHSVTTINGSTIITQNGISETFPGGIFAQRTNSGTFED
                     DQFAVVPELGVMAGYQINPCWRVTVGYTFIYWSQVARAGDQIDREL"
BASE COUNT          416 a          776 c          744 g          438 t
ORIGIN      
        1 aaagccggtc tgcacctcgt cggcaatgca caggccgccg tgcttccgca caatgtcata
       61 gacgatctga aagtattccg gcggcggcgt gacggcgccc ccgacgccct gaatcggctc
      121 ggcgatgaag caggcgattt cgcccgatgt ttccgtgcgg atgagcgtct ccacatcgcg
      181 ggcgcattgc acgttgcagc tcggatactc cagccccagc gggcagcgat agcaatagcc
      241 gggaagggcg ttcttgacgc cgatcgtcgg gctggacctg aatttccagg tcgcatggcc
      301 ggtaagcccc atcgtggcct gcgttccgcc gtggtagccg ttgcggaggc tgatcacctc
      361 ctgattgccg gtgaattcgc gggccgagag aatcgcgacc tcgtttgctt cactcccgga
      421 gttcgtgaag tagctcaccg acaggccgct gccggcaggc atgtggtcgg ccagcttctg
      481 ggcgaactgg ccgatcgtcg gatgcaggta gatcgtcgtg gtgtgctgca agcggccaac
      541 ctgctcgcgg acgcgctcga cgatctccgg atggcaatgt ccgaccgaga ccgtcacgat
      601 gccggcaaag gcgtcgagat actgcttgcc ccgctcgtcc cacacatact gcatgtgtcc
      661 ttcgacgagc atcagcggct cgcgatagta cgtgatcaga ccgggagtga ggtactgggc
      721 gcgtaatgcc agcacttcgt ccttgggcgg accggcatag ggctgcggaa cgtgcgaagt
      781 cccgggtggg ccggcgagga tgggatcgcg agcgggcatt cgacgtaaaa gctgtgggat
      841 tcgggcgata tgcacggacg cggcaccgct ggtcattgta gcccagccgc atgctgctag
      901 cgacatcgct cgcctggcgg ttcgcgaaga tttgcccttc gcgccgcctg ccgtattcag
      961 tacaagccca cgctcgtctc atcaccgacg tgccgggcga ttgtgcgcaa agcgggaact
     1021 ttgttgcgcc gccgggccca tccaggaggg agttggacgt gagtccctca tccatcgcgc
     1081 attgcgcggc catttggctg gcgctgttcg gttcgacgta cgcgcagagc cagggccctg
     1141 cgccgggcag gcagccggct ccggttcgca cctcgccgcc cgccgcacaa caggccgaaa
     1201 aaacgcctgc ccagcgcccg gttcagccgg cggcgcatca ggaggtcctg ccggaggtcg
     1261 ctccgctgcc gggcgaacag atgtattacg aaggtgaagg gcccgttttc gccgacgact
     1321 ggggaacctg cggccccggc ggctgcaatt tctgcggcca gcgcggcccc ggctgcgggc
     1381 cgtttggcca aggctgcttg agcggcgtct atgtccgggc ggaatacctt ctctggggcg
     1441 ccaaaggggc gacgctgccc ccgctggtga cctccagccc gcctggcacc gcgcaaaacg
     1501 tcgccggtat tctcggcgag cccgacacgg tgatcctctt tggcgacgaa actgtcaaca
     1561 acgacctccg ttccggcgga cgcctggtcc tcggctggtg gttcgatccc tgccgccggc
     1621 tgggcatcga aggcgattac ttttcgctcg acgatgaatc gaccgacttc cgggctagtt
     1681 ccaccggcac aacgatcatc ggcgtgccgt tctacgacgt ggtgcaaggc atcgagaatg
     1741 tcaacctggt cgccttcccc aacctgatcc aaggcaccat tgctgccgac taccgcacga
     1801 ctttccaggg cgcaggcatc cgcgccatgt acaacctcgc ctgcggcgag gggggcggct
     1861 gtagctggat caccggctgc cccgtgcata ccggattccg gttcgacatg ctcctgggct
     1921 accgctttgt gcggctcgac gacaacttca cgctggtcga aaactccgtc tccctcagca
     1981 cggccaatcc cggctcgttc aacatccgcg accagttcga caccgaaaac cagttccacg
     2041 gcatcgatct gggcacgtcg ctcagcttct gcaaaggctg cttctcgctc gagatgctct
     2101 cgaaaatcgc cctcggcaat acgcacagcg ttacgacgat caacggcagc acgatcatca
     2161 cgcaaaacgg catctcggaa acgttccccg gcggcatttt tgcccagcgg acgaacagcg
     2221 gcacgttcga ggacgaccag ttcgccgtcg tgcccgaact gggcgtgatg gctggctatc
     2281 agatcaatcc ttgctggcga gtgaccgtcg gctacacgtt catttactgg agccaggtcg
     2341 cccgcgccgg cgatcagatc gaccgcgagc tgaa
//
LOCUS       DASUGB010000967         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_526984, whole genome shotgun sequence.
ACCESSION   DASUGB010000967 DASUGB010000000
VERSION     DASUGB010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526984"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFV87_18290"
     CDS             <1..999
                     /locus_tag="VFV87_18290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5105778.1"
                     /translation="SPLSPRGRGAGGEGAAFPLSPAPCPPSPPAWQPGDPVALLLHGL
                     GGCHQSGYMQRCAAKLNERGYRVFRMDLRGYGAGFPLARHPVHAGRSEDAAAVLNYVI
                     ELCPDSPIHLVGFSMGANIVLKLAGELGQLAPANLASVMAVAPPIDLVECSRNMQRRL
                     NHFYDRRFVRSLLALIERRRQLVPGALHRPLDPPPRRLVDFDERFTAPLSGFESAHDY
                     YTRASSGPLLCHIAVPTLIVTAANDPIIPVGPFERASYSPTTNLVVTPCGGHLGFIAR
                     PGLDPDRRWLDWRVLEWIESHARVPRAAHTKATALTSKTGDCFKTAAYAPGPAGQP"
     gene            complement(956..1447)
                     /locus_tag="VFV87_18295"
     CDS             complement(956..1447)
                     /locus_tag="VFV87_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5105779.1"
                     /translation="MGQKERLRRQLVAVRQNSERLLADFRAPEEWTFQVHPSCNHALW
                     FAGHMAISDNFFLSLVAPDRSQPSPEYQAKFGMGSQPTNQPEAYPAPEVVLQAMRERR
                     QALLEALDRLSDADLARKTPAGAPDFLPDVASVFELAIWHEGQHSGQVTVARRVLGHK
                     PLF"
     gene            2044..>2890
                     /locus_tag="VFV87_18300"
     CDS             2044..>2890
                     /locus_tag="VFV87_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5105780.1"
                     /translation="MQATSPWRDLFEESYKPMNLRGCSPLTPGGHLAAINGLEAYWRS
                     LEAGRPGKTLRIGDVSETLIAKYLDHLLVVKRRRRATHNKHLRHLTAQWNWALSEGLV
                     PRKLRLTQIKEEERDPDCWSQEEFTRLLFVAAELRGYIGDVPACYWWPALILMVYSTG
                     VRIDAIMRIRSADLDLEQRVVRVQAETQKDKAEQRFTLLPDVCGVLGRIRPERLPCVF
                     DDWPLDRTAKARARNGWDALRRAFRKLLAKAELPTTSRDLWHKLRRTFATEVTLSSGL
                     KVAQAL"
BASE COUNT          473 a          996 c          930 g          491 t
ORIGIN      
        1 tctcccctct cccctcgtgg gagaggggcc gggggtgagg gggctgcttt tcccctgtcc
       61 cctgccccct gtcccccgtc ccctcctgcc tggcaacccg gcgatcccgt cgccctgctc
      121 ctgcacggac tcggcggctg ccaccagtcg ggctacatgc aacgctgcgc cgcgaagctc
      181 aatgagcgcg gctaccgcgt cttccggatg gacctccgcg gctatggcgc gggattcccg
      241 ctggcgcggc atccggtgca cgccgggcgc agcgaggatg cggccgcggt gctgaactac
      301 gtcatcgagc tgtgccccga ttcgccgatc catctggtcg gcttttcgat gggcgcgaac
      361 atcgtcctca agctggcggg cgagctgggg cagctcgcgc cggccaatct ggcgagcgtc
      421 atggccgtcg ccccgccgat cgatctcgtc gaatgttcgc gaaacatgca gcggcggctg
      481 aaccatttct acgaccgccg gttcgttcgc tcgctcctcg cgctcatcga acgccgcagg
      541 caactggtgc ccggcgctct gcaccgcccg ctcgatccgc cgccgcgccg cctggtcgat
      601 ttcgacgaac gcttcaccgc cccgctctcg ggcttcgaga gcgcccacga ctattacacc
      661 cgtgccagca gcggcccgct cctctgccac atcgccgtac ccacgctcat cgtgacggcc
      721 gccaacgacc cgatcatccc ggtcggcccg ttcgagcggg ccagctactc gccgacgacg
      781 aacctggtgg tcacgccctg cggcgggcat ctcggcttca tcgcccgccc cggcctcgac
      841 cccgaccgcc gctggctcga ctggcgggtc ctcgagtgga tcgagtcgca cgcccgcgtc
      901 ccacgcgctg cgcataccaa agccacagcg ctgacatcga aaactggcga ctgcttcaaa
      961 acagcggctt atgccccagg acccgccggg caaccgtgac ttggccgctg tgctggccct
     1021 cgtgccagat cgccaattcg aagaccgagg ccacatcggg cagaaagtcc ggcgctccgg
     1081 cgggggtctt gcgggccaaa tcggcgtccg acagtcgatc gagcgcttcc aggagcgctt
     1141 gccgccgctc ccgcatcgcc tgcaggacga cttccggcgc aggataggcc tccggctggt
     1201 ttgtcggctg cgagcccata ccgaatttgg cctggtattc cggcgacggc tgcgaccgat
     1261 ccggcgccac gagggaaagg aaaaagttgt cgctgatcgc catgtggccg gcgaaccaca
     1321 acgcgtggtt gcaactcgga tgcacctgaa acgtccattc ctccggcgcg cgaaagtcgg
     1381 ccagcagccg ttcgctgttc tgcctcaccg cgacaagttg cctccgcagt cgttccttct
     1441 gtcccatcac gtcactttct gcattttgga agtccacgct ccgcctgatc tgagttgcca
     1501 ggtcacgtcg aaaactcggc acagcgagaa agcattaggg ccgcccggac tcgaaccggg
     1561 aaccaaagga ttatgaggcc cacgtgtttt gccgatccat tctcacctcg gctcgagtgc
     1621 agaatcaatc ggcttgtgag ggtttgaaca cttgcggttc gcgggatgcg agcgagtcgc
     1681 gcttgacaag tcagggtggg aaagtatctt ggcactcgct gattcaaaaa ttcggtagag
     1741 ccctctctcg cgatcttccc tagcccagct cttctgggcg cgaaaggccg cggccagtgg
     1801 ttctacccct ggccggcagg tgcctaccga cacctggaca gccggtcttg cgtgatccag
     1861 ttccgctcgg cgcgctagcg ccggtcgtac cgtttgcggt gcgccggtcg ctggccgctg
     1921 cgcgacgact gaggtgatcc atgcccagag cgggaaggcc tccgcgacgc aacggacgcg
     1981 agctgcggcg cgtgcgcgcg tcggaagcgg gagccgtcgc atcgcgccgc cggagaaccg
     2041 gcaatgcaag cgacctcacc ctggagagac ctcttcgaag aatcctacaa gccgatgaac
     2101 ctccgcggct gctcgcccct gacgccgggc ggacacctgg cggcgatcaa cgggctggaa
     2161 gcgtattggc gatcgctcga agcggggcgg cccggcaaaa cgctgcggat cggcgacgtg
     2221 agcgagacgc tgatcgccaa gtacctggac catctcctgg ttgtcaaacg ccgccggcgg
     2281 gcgacgcaca acaagcacct gcggcacctg accgcccagt ggaactgggc gctctccgaa
     2341 gggctggtgc cgcggaagct gcggctgacg caaatcaagg aagaggagcg ggatcccgac
     2401 tgctggtcgc aggaggaatt cacgcggctg ctgtttgtcg cggcggaact gcgcggctac
     2461 atcggcgacg tgcccgcctg ctattggtgg ccggccctga tcctgatggt gtattcgacg
     2521 ggggtgcgga tcgacgcgat catgcggatc cgctcggccg acctggacct ggagcagcgg
     2581 gtcgtgcgcg tgcaggcaga aacgcagaag gacaaagccg agcagcgatt cacgctgctg
     2641 ccggacgtgt gcggggtgct cggccggatt cggccggagc ggctcccgtg cgtcttcgac
     2701 gactggccgc tcgatcgcac ggccaaggcc cgcgcgcgaa acggctggga tgctttgcgg
     2761 cgcgcctttc gcaagctgct ggccaaggcg gagttgccga cgaccagccg cgatctgtgg
     2821 cacaagctgc ggcggacctt cgcgaccgaa gtgacactgt cgtcgggcct caaggtggcc
     2881 caggcattac
//
LOCUS       DASUGB010000968         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_527014, whole genome shotgun sequence.
ACCESSION   DASUGB010000968 DASUGB010000000
VERSION     DASUGB010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527014"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..2634
                     /locus_tag="VFV87_18305"
     CDS             232..2634
                     /locus_tag="VFV87_18305"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5105781.1"
                     /translation="MEVKSRVFRPFAEWVIAHRIAVIIGIFVITAFLVTRIGTLQLDT
                     NPDSWAPQKHVYVETTKQLKKIFGGGNVVLIGITPKQGDIYQPAVLAKIKRIQEGIEQ
                     IPHAVRHNVVSLAARKVKHIKGGPEGMEVREMLETMPQTPAEVEKLKAAVASMPIYIN
                     SLVSPDGKSAAIVADFKQDATVPNFVAMLEGMRKVVERERDATVDIHYGGSPVIGEAA
                     DLQFMKMPMFFGGALLVIMLILLWSFRSVQGMLLPMVTGILSVIWSLGLMAVLGVHMD
                     PLNTTTPILILAVAAGHAIQILKRYYEEYNRLLAAGSTPREANRTAVVESMVRVGPVM
                     ITAGLIASITLFSLAGTGIPMVQHFGVFAGCGVLATMVLEMTIIPSLRSLLRAPKKRE
                     TANERKAGILDRFLIRVADNLVGGRAPRLVIGGLVALAIVGAGTLYLRVDNNFRLYHR
                     PDSVLRIDDRILNDTFGGTNTIQFLVQTPTADSLKDPKVLQGISDLQAFFERQPNVGK
                     TQSIADLIKRMNQAMHGDDPNYYTIPESRELVAQYLFLYSLSGDPQDFDSFVDNDYQR
                     ADVWTYFKDDSTTYADSVAKKAQAIVAQSFPPGVTVSMGGTLPDAVALNEVIVKDKFR
                     NMVQMALVVFVLGAIAFRSLAGGLFVVTPLLAVALANFGVMGWTNTPLDISAMISAAL
                     AIGIGADYEIYLLYRFREELARSGDILTATRDSLLTSGKAVLFVAISIMGGYAVLQFS
                     DFAFFNQISNMVMATMAVSAFFALFFLRALMMIFKPRFVFGNGRDTQLGQPVAVGQGA
                     AK"
     gene            2685..3443
                     /locus_tag="VFV87_18310"
     CDS             2685..3443
                     /locus_tag="VFV87_18310"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX5105782.1"
                     /translation="MSVDARATGAEPNAHEIMEKNFFVSKLKSMKAEAYMVLTNDKGQ
                     KRERKSTSLVKLQPNGIDSKILVKFSTPNDIKGTSVLQIEHSEGDDDLWIYLPALKKS
                     RRLVASNKKDSFVGSDFSYGDITLPKVDLYRHALLRSEAIDAQDCYVVESVPANETVK
                     TNSGYSKKITWVRKDNFLETKVEYYDVAGRLLKTQRVKDHILIEPDTQRWFAKDREMT
                     NHQTGHQTDFRFDKLEPGAKVDEALFSIRYLERE"
     gene            complement(3575..4792)
                     /locus_tag="VFV87_18315"
     CDS             complement(3575..4792)
                     /locus_tag="VFV87_18315"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105783.1"
                     /translation="MFIDITPLRNNRDFRLLFAGQLISFLGSMVSYMAVPYQVYQLTQ
                     SNAMVGALGLAQFGPVVVFGLLGGAYADRINRRRLMLVCESLMAFLLLLLLANSLLPQ
                     PSLVAIFVLVAALQGVAGFHTPAMEALTQKLVTPTEYAAVGALASFRFSTGAIAGPLL
                     GGVLIALFGLQGAYLFDLLSFTGAVICIALMRKTPDPETTPHSPLADAAIGFKFALSK
                     PELVGTYFIDIAAMLFAFPVALFPAIAAQWGDARMVGLLFSSMAIGALLATLFSGWSG
                     RVQRQGRMVVIAAVLWGLFVVAAGLVSTPWLLVLFLILAGAADMVSALFRGIIWNYSV
                     PNAMRGRLAGIAMISYMSGPLLGNTRAGWVAAKSSVTFSLWSGGLACVLAVFMTSLLL
                     PKFWAYRASQDAG"
     gene            complement(4907..4983)
                     /locus_tag="VFV87_18320"
     tRNA            complement(4907..4983)
                     /locus_tag="VFV87_18320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4947..4949),aa:Pro,seq:cgg)
     gene            complement(5135..>5468)
                     /locus_tag="VFV87_18325"
     CDS             complement(5135..>5468)
                     /locus_tag="VFV87_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105784.1"
                     /translation="VTGRLGNVNGAIRLDAAHVGGGITTVNGNIDIGANSRVEGGIVM
                     EKSSSSFFNFSWGKRSMPRVVIGPGATVDGTLRFEREVELYVSETAKIGPVQGAEPVK
                     FSGNEPPR"
BASE COUNT         1196 a         1601 c         1668 g         1003 t
ORIGIN      
        1 atcccgttgc ggatgtgctc atgaaaaaga ccacgttcgc tcgcgaggac tcgatccggt
       61 gcagctgtaa gttcgtaaat gcaagtttga ttatgtaact tgcaaatacg aaggcacaaa
      121 tgtaaatatt tggatcgacg ctaacggctc ggcggggcgg cagagtgcaa ggtgcgtctg
      181 ttctgggtga cgcgtcgctt tttggaaagc tactagatcc gggagagttg aatggaagtc
      241 aagtcgcgcg tgtttcgtcc gttcgccgag tgggtcatcg cccatcggat cgcggtgatc
      301 atcggcattt tcgtgatcac ggcgtttctc gtcacccgca tcggcactct gcagctggat
      361 accaatccgg attcctgggc accgcagaag cacgtttacg tcgagacgac caagcagctg
      421 aaaaagatct tcggcggcgg caacgtcgtg ctcatcggga tcacgcccaa gcagggcgac
      481 atctatcagc cggcagtgct ggccaagatc aagcgcattc aggagggtat tgaacagatc
      541 ccgcacgccg tgcggcacaa cgtggtgagt ctggccgcgc gcaaggtcaa gcacatcaag
      601 ggcggaccgg agggcatgga agttcgcgag atgctggaga cgatgccgca gaccccggcg
      661 gaggttgaaa aactcaaggc ggcagtcgcc tcgatgccga tctatatcaa ctctctggtc
      721 tcgccggacg gcaagtcagc ggcgattgtc gcggacttca agcaggacgc gacggtgccg
      781 aatttcgtcg cgatgctgga ggggatgcgc aaagtcgtcg agcgcgaacg cgatgcgacc
      841 gtggacatcc actacggcgg atcccccgtg atcggggaag ccgccgatct tcagttcatg
      901 aagatgccga tgttctttgg tggggcgcta ctggtgatca tgctcattct gctgtggtcc
      961 ttccgcagcg tgcagggaat gctcctgccg atggtcacgg gcatcttgag tgtgatctgg
     1021 agcctgggac tgatggcagt gctgggggtc cacatggacc cattgaacac caccacgccg
     1081 attctgattc tggcggtggc cgcgggccat gccattcaga ttctcaaacg gtattacgag
     1141 gagtacaacc ggctgctcgc cgccgggtcg accccgcgtg aagcgaatcg cacggcggtg
     1201 gtggaatcga tggtccgggt gggtccggtg atgatcaccg cagggttgat cgcgtccatc
     1261 acgctcttct ccctggcggg caccggcatc ccgatggtgc agcacttcgg agtgtttgcc
     1321 ggttgcggcg tgctcgccac gatggtcctg gagatgacga tcattccgtc gctgcgttcg
     1381 ctgctgcgtg ccccaaaaaa gcgggaaacc gccaacgagc gcaaggccgg tatcctggac
     1441 cgctttttaa tccgggtcgc ggacaatctg gtgggcgggc gtgcgccgcg gctcgtgatc
     1501 ggcggactcg tcgcccttgc gattgtggga gccggaacgc tgtatctgcg cgtggacaac
     1561 aacttccggc tctaccaccg gcccgatagc gtgctgcgca tcgatgaccg gatcctgaac
     1621 gacaccttcg gcggcaccaa cacgatccaa ttcctggtgc agacccccac agcggattcc
     1681 ttgaaagatc cgaaagtgct gcaggggatc tcggatctgc aggccttctt cgagcgccag
     1741 ccgaacgtgg gcaagaccca gtcgatcgcc gacctgatca agcgcatgaa tcaggccatg
     1801 cacggcgatg atcccaacta ctacaccatc cccgagagcc gcgagctggt cgcgcagtat
     1861 ctgttcctgt actcgctgtc gggggatccg caggatttcg acagctttgt ggacaatgac
     1921 tatcagcgcg cggatgtgtg gacgtacttc aaggatgaca gcaccacgta tgccgactcg
     1981 gtggcaaaga aagcccaggc gatcgtcgcc cagagctttc ccccgggggt gacggtgagc
     2041 atgggaggca ccttgcccga tgcggtcgcg ctcaacgaag tgatcgtaaa agacaagttc
     2101 cggaatatgg tgcagatggc cctggtggta ttcgtgttgg gcgccattgc gttccgctcc
     2161 ctggcgggag ggctcttcgt ggtcacgcca ttgctggcgg tagccctggc gaacttcggg
     2221 gtcatgggct ggacgaatac gccgctggat atcagcgcga tgatatcggc ggccctcgcg
     2281 attggtatcg gcgcggacta cgagatctat ctgttgtacc ggttccgcga ggagctggcc
     2341 cgcagtggag acattctcac ggcgacgcgc gattcgttgc tgacctccgg caaggcggtg
     2401 ctgttcgtgg cgatttcgat catgggcggc tacgcggtgc tgcaattctc cgacttcgcg
     2461 ttcttcaacc agatctccaa catggtgatg gcgacgatgg cggtcagtgc gttcttcgcg
     2521 ctgttcttcc tgcgcgcgct gatgatgatc ttcaagccgc ggttcgtctt cggcaatgga
     2581 cgggatacgc agctcggtca gccggtggcg gtgggacagg gggctgcaaa gtgagccgga
     2641 gccgcggtac aacgctcttt gccgttctgc tggcagtggc cctggtgtct gtagacgcca
     2701 gggccacagg ggccgagcct aacgcgcacg agatcatgga gaagaacttc tttgtctcga
     2761 agctcaaatc catgaaggcc gaagcctata tggtcctgac caacgacaag gggcagaagc
     2821 gcgagcgcaa aagcacgagt ctggtgaaat tgcagcccaa cgggatcgat tcgaagatcc
     2881 tggtgaagtt cagcacgccc aacgacatca agggcacgag cgtcctgcag atcgagcaca
     2941 gcgagggtga cgatgatctg tggatctatc tgccggcgtt gaagaagagc cggcgcctgg
     3001 tggcgagcaa caaaaaggac agcttcgtcg gctcggattt ttcctatggg gacattacgc
     3061 tgccgaaggt ggatctctat cgccacgccc tgttgcgttc cgaagcgatc gacgctcagg
     3121 actgttacgt cgttgagtcg gtgccggcga acgagacggt caagacgaac agcggctata
     3181 gcaagaaaat cacgtgggtc cgcaaggata attttctaga gaccaaagtg gagtactacg
     3241 acgtggcggg gcggctgctc aagacgcagc gggtgaagga ccacatactg atcgagccgg
     3301 acacccagcg ctggttcgcc aaggaccggg aaatgacgaa tcatcagacc ggccaccaga
     3361 ccgacttccg cttcgacaag ctggagcccg gtgcgaaggt cgacgaggcg ctgttctcga
     3421 tccgctatct tgaacgcgaa tgaccggcga gcgcgaatga cacacacgtc cacgcgaacg
     3481 atgcctccgg caagtgtcgg actcgtcaac tcaggcgcgg tatccgtgta aacacggatc
     3541 gcagaatccg tagatctacg gatatacaca tgtctcagcc ggcatcctgg cttgcgcgat
     3601 aagcccagaa cttgggcaat aacaggctgg tcatgaacac ggccagcacg cacgccagac
     3661 cgccgctcca gaggctgaac gtaacgctgc tcttcgcggc gacccatccc gcgcgcgtgt
     3721 tgcccagcag tggaccactc atataggaaa tcatcgcgat gcccgccaga cgtccgcgca
     3781 tggcattggg cacagagtag ttccagatga tgccgcgaaa gagcgcgctc accatgtcgg
     3841 ccgctccagc aagaatcaga aacaacacga gcagccaggg agtcgacacc agtccggctg
     3901 cgacaacaaa gaggccccat aacactgcag cgatcactac cattcgcccc tgccgttgca
     3961 cgcggccgct ccagccactg aaaagcgtgg ccagcagagc gccaatcgcc atggatgaga
     4021 acaacaaccc caccatccgt gcatcgcccc attgcgcagc aatggcaggg aacaacgcga
     4081 ctgggaacgc aaacaacatc gcggcgatgt cgatgaaata agtgcccacg agctcgggct
     4141 tcgaaagcgc gaacttgaat ccgatagcgg cgtcagccag gggcgagtgc ggcgtagtct
     4201 cgggatctgg cgtttttctc atgagcgcga tgcatatcac ggcgcccgtg aacgagagca
     4261 aatcgaacaa ataggccccc tgcaaaccaa agagggcaat cagtacaccg cccagcaagg
     4321 gccctgcaat cgcgcctgtc gaaaagcgaa agcttgcaag agcgcccacg gctgcgtact
     4381 cagtcggtgt gacgagcttt tgcgtcaatg cttccatggc gggtgtgtga aagcccgcaa
     4441 caccctgcag agccgccacc aacacaaaaa tcgccaccag cgacggctgc ggcagcaagc
     4501 tgttcgccaa cagcagcagc agaagaaatg ccatgagcga ctcgcacacc agcatcaagc
     4561 ggcggcgatt gatgcgatcc gcgtaagcgc cgccgagcag gccgaacacg accaccggcc
     4621 cgaactgcgc gagacccagt gcgcccacca tcgcattgga ttgcgtcaac tgataaacct
     4681 ggtacggcac ggccatgtaa ctgaccatgc tgcccagaaa ggagatcagc tggccggcga
     4741 agagcaggcg aaagtcgcga ttgttccgaa gcggtgtgat gtcaatgaac atgaggcaga
     4801 gccaccgcct gatttgaccc ggtaactaag gaccagcaac cccgcgggac cacaccgact
     4861 gaagcccgcg aaatcaaatg gcgggaggac agtagcttct atgagatggt cgggatgaga
     4921 ggatttgaac ctccgactcc tacgtcccga acgtagtgct ctaccaggct gagctacatc
     4981 ccgatcatat tggcgggtgc accctctggc ctttttcccc ggcggccggg acgcgcaaaa
     5041 acaacgcgat acgccggaat ggaccagaag cttgcgtggg ccgcgcagtc tactcgaagg
     5101 ctccggggag attcaatcga ccggcaaggg gccactacct gggtggctca tttcccgaaa
     5161 acttcaccgg ctcagccccc tgtacggggc cgattttggc tgtctcgctt acgtacagct
     5221 cgacctcgcg ctcaaaccgc agagtgccgt ccaccgttgc gcccggaccg atcacgaccc
     5281 ggggcatgct ccgctttccc caggaaaaat tgaaaaaaga cgaggacgat ttctccatga
     5341 cgatgccgcc ttccacgcgg gaattcgcac cgatatcgat gttcccattg accgtcgtga
     5401 ttccgccgcc tacgtgggca gcgtccaggc gaatggctcc attcacgtta ccgagccggc
     5461 ccgtgaca
//
LOCUS       DASUGB010000969         6273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_108073, whole genome shotgun sequence.
ACCESSION   DASUGB010000969 DASUGB010000000
VERSION     DASUGB010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108073"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFV87_18330"
     CDS             <1..318
                     /locus_tag="VFV87_18330"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5105785.1"
                     /translation="GLCHEIDAKGQYLLASPLHPVATATSVRIRDGKRLVTDGPFAET
                     HEQLGGFYLIDVPDIETAIEIAGRHPGAPHGTVEIRPMLELPGLPAAATASRESLQID
                     YGG"
     gene            352..993
                     /locus_tag="VFV87_18335"
     CDS             352..993
                     /locus_tag="VFV87_18335"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEX5105786.1"
                     /translation="MNAKYRMVAASLALLALGTIAFAEPSKEAKPAAAAEPKLPPGWT
                     EADLKAFMASATPGDMHKRLVGDAGTWQGKNTMWMAPGAEPLETQATTIITPILDGRF
                     IRLEMKGEMPGMGPYHGLAVHGYDNITKKFISTWIDNMSTSMAHGEGELSKDGKTLTW
                     TYTCNCPLQDKPMTMRQIETTTGPGKKTIVMFGAEPKSGEEYKMMQIELTKVK"
     gene            1153..1512
                     /locus_tag="VFV87_18340"
     CDS             1153..1512
                     /locus_tag="VFV87_18340"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX5105787.1"
                     /translation="MPYVDGFVLPVPKKKLKVYQKMAEMGGRIWKEHGALEYRECIGD
                     DLDVMFGLPFSKSVKLKKGETVVFAWIVYKSRAHRDKVNAKVMKDPRLGQGMESKEMP
                     FDMKRMYYGGFKTLVDM"
     gene            1532..2074
                     /locus_tag="VFV87_18345"
     CDS             1532..2074
                     /locus_tag="VFV87_18345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5105788.1"
                     /translation="MVTNILLGTAAALVVLLIGLVLIVAMRPADFRISRQLALTAKPE
                     AVFPHVNELAKWRAWSPWEELDPGLRRTYEGPAAGEGASYAWSGNNKVGEGRMTITES
                     QPSDLIRLRLEFIKPFQAINTTEFKFERNERGTLVIWSMLGRNSFMAKAFGLMMDMDK
                     LIGKDFEKGLAKLKSVAETT"
     gene            2094..2516
                     /locus_tag="VFV87_18350"
     CDS             2094..2516
                     /locus_tag="VFV87_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5105789.1"
                     /translation="MSTATPTPPANPKWMTWLGWVLTILPSLLLVFSAVMKFVGPPEV
                     KDGFTKAGWNPNLLLPLGLTELACTILYLVPQTSTLGAVLLTGYMGGAIAHHLRLGEP
                     IAFQVAFGVVIWLGLFLREPRLRAILPIRSPNATTTRP"
     gene            2553..3740
                     /locus_tag="VFV87_18355"
     CDS             2553..3740
                     /locus_tag="VFV87_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX5105790.1"
                     /translation="MSSVRVLIGTKKGAFIATADGKRQKWTISTPQFGGWEMYHLKGS
                     TVDPNRIYASQSSGWFGQVISRSDDGGKTWTTPGSKPEELQSPEGMPKGESNKFVYNT
                     EPPTGKPLTTHQWYDGTQHAWEFKRVWHLEPSLTDPDTVYAGVEDAALFRSTDGGKTW
                     QEIASLRGHGTGPKWSPGAGGMGLHTIVQDPKNPQRMYIAISAAGAFRTDDGGQSWKA
                     INKGLKSPYLPEMTPEVGFCVHRIALHPSNPDVLFMQLHQGGGVYRSDNAGDQWQEVN
                     GNLPTDFGFPIDVHAHEPKTIYSIPMDPYLRVPPGGKLRVWRSTAGGNEWEPLTKGLP
                     QENCYVNVLRDAMCVDSLDDCGIYFGTTGGQVYCSPDGGNNWHAIARDLPYVQSVEVQ
                     TLS"
     gene            3737..4033
                     /locus_tag="VFV87_18360"
     CDS             3737..4033
                     /locus_tag="VFV87_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5105791.1"
                     /translation="MIRVVIPYHLRLLSGVTGEIALEVEPPVTQRAVLDALEARYPML
                     KGTIRDHVTFKRRAFLRYYACEQDLSLEEPDAQLPAAVASGKEPFLVIGAIAGG"
     gene            4101..5450
                     /locus_tag="VFV87_18365"
     CDS             4101..5450
                     /locus_tag="VFV87_18365"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105792.1"
                     /translation="MTAQENATDALAASASPSADAPIHPNHFPLRLIVAGIVGNVMEW
                     YDFAVYGYFATSIGQHFFPSDNPSTSLISAFGAFAAGFLMRPLGGLVFGHIGDHFGRK
                     AALTLSVLAMAIPTFLIGVLPGHQVLGTGSAVLLVVLRMIQGLSVGGEYTTSIVFLFE
                     GAGQKHQGFASSWSGFGAVGGILLGSAVGAVVTGLLPEETVHNWAWRLPFLVGLAVGL
                     AGLYVRGHIPEPPRQPREKMGRSPVWDALQTEWRTILRLALLNTVNGIGFYMAFVFLV
                     TYMKSVGGLTEAMALEINTVNMAVLLAMFPLAGWLSDRIGRIPLLVVGIGGMLLFSWP
                     LFWALHHPSAAWSFVGQLGFAVCIGLFGGVIPVAMVEMTPAAVRCSAISIGYNLCVGV
                     LGGTTPMVAAWLMKETRDVLAPAWYLMVAAAISLGVVVVPLLFSRKHTATKSCNESG"
     gene            complement(5442..5516)
                     /locus_tag="VFV87_18370"
     tRNA            complement(5442..5516)
                     /locus_tag="VFV87_18370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5479..5481),aa:Pro,seq:tgg)
     gene            complement(5612..>6273)
                     /locus_tag="VFV87_18375"
     CDS             complement(5612..>6273)
                     /locus_tag="VFV87_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5105793.1"
                     /translation="RLRAAKRAGWTTIPARVRDADDRLVAELAIVENLQRKDLNPVEK
                     ALSFKRYLDEHRCTQDDLAKRLKIDRSTIANLMRLLELPPSILSGLSTGSITAGHARA
                     LLPLGDEHEQVALAKRIEAEGLSVRDVERIVGEQVGDEDGEDVEILPGPGSKARTRSE
                     HVASLEQEIRHALGLKVELRANHRGRGKLVIHFQNHDEFDHLREQLTDAAPPATRRKS
                     A"
BASE COUNT         1186 a         1961 c         1932 g         1194 t
ORIGIN      
        1 ggcctgtgcc acgagatcga cgccaagggg caatacctcc tcgcgtcgcc gctgcatccc
       61 gtcgccacgg ccaccagtgt ccgcattcgc gatggcaagc ggctggttac cgacggcccg
      121 tttgccgaga cgcacgaaca actcggcggc ttttacctga tcgacgtgcc ggatatcgag
      181 acggcgatcg aaatcgccgg ccggcatccc ggcgcgccgc acggcaccgt cgagatccgg
      241 cccatgctcg aattgccggg cctgcccgca gcggccactg ccagccgcga atcattgcaa
      301 atcgactacg gcggctagac gccgccgcta cttcacttcc cggaggttcc tatgaacgcc
      361 aaatatcgaa tggttgccgc ttcgcttgca ttgctggccc tcggcacgat cgccttcgcc
      421 gagccgtcta aggaagccaa gccggccgcg gctgccgagc cgaagctccc gcccggctgg
      481 accgaggcgg acttgaaggc cttcatggca tcggcgacgc ccggcgacat gcacaagcgg
      541 ctcgtcggcg acgccggcac ctggcaaggc aagaacacga tgtggatggc ccccggcgcc
      601 gagccgctcg aaacacaagc cacgacgatt attacaccga ttctggacgg ccggttcatc
      661 cgcctggaga tgaaaggcga aatgcccggc atggggccgt atcacggcct ggccgttcac
      721 ggctacgaca acatcaccaa gaaattcatc tccacctgga tcgacaacat gagcaccagc
      781 atggcccacg gcgaagggga gctgtcgaag gacggcaaga cgctcacttg gacgtacact
      841 tgcaattgcc cgctgcagga caagccgatg accatgcggc agatcgaaac gacaaccggc
      901 cccggcaaaa agacgatcgt catgttcggc gccgagccca agtcgggcga agagtacaag
      961 atgatgcaga tcgaactgac caaagtgaag tagcctgggt tgctccacag aaagcaatgg
     1021 aagccgcgga tggaacacag atgaaacacg gatcgagcaa acaatgagtc gattccccgt
     1081 ttgcatccgt gttcgatccg tgtttcatcc gtggctacaa atccgccctt ccaacattcc
     1141 tggaaattca ccatgcctta cgtcgatggt ttcgtcctgc cggtcccgaa gaagaaattg
     1201 aaggtctatc aaaaaatggc cgagatgggc ggcaggatct ggaaggagca cggcgcgctc
     1261 gagtatcggg agtgtatcgg ggacgatctg gatgtaatgt tcggtttgcc gttcagcaag
     1321 agcgtcaagc tcaagaaagg ggagacggtt gtcttcgcct ggatcgtcta taaatcgcgc
     1381 gcccaccgcg acaaagtgaa cgccaaggtg atgaaagacc cgcgcctcgg ccaggggatg
     1441 gaaagcaagg aaatgccctt cgacatgaag cggatgtact acggcggctt caagacgctc
     1501 gtggacatgt gattcacccg gattcccacc catggtcacc aacatcctgc tcggaacggc
     1561 cgcggcgctt gtcgtgctct tgatcgggct cgtcctgatc gttgcaatgc ggccggcaga
     1621 ttttcgcatc tcgcggcagc ttgcgctgac agccaagccc gaggccgtgt ttccgcacgt
     1681 caacgagctg gccaaatggc gggcctggtc gccgtgggaa gagctggatc cgggcctgcg
     1741 gcgaacctac gaagggccgg cagcgggcga aggggccagt tatgcctggt cgggcaacaa
     1801 taaggtcggt gaagggcgta tgaccattac cgaaagccag cccagcgacc tgatccgctt
     1861 gcggctcgaa ttcatcaagc cctttcaggc gatcaatacg actgaattca agttcgagcg
     1921 gaacgaacgc ggcacgctcg tcatctggag catgctcggc cgcaacagct tcatggccaa
     1981 ggccttcggc ctgatgatgg atatggacaa actgatcggc aaggatttcg agaaaggcct
     2041 cgcgaagctg aagagcgtcg ccgagacaac gtaaccgccc ggaattcccg cgtatgtcaa
     2101 ccgcaacacc aactcccccc gccaatccca agtggatgac ctggctcggc tgggtcctga
     2161 cgatcttacc atcgctgctg ctcgtattca gcgccgtgat gaaatttgtc ggcccgccgg
     2221 aagtgaaaga tggatttacc aaggcaggtt ggaacccgaa cctgctcctt ccgctcggac
     2281 tcacggaact ggcgtgtacc atcctctatc ttgttccgca gacaagtaca ctcggtgcag
     2341 ttctgctcac gggatacatg gggggcgcga tcgcccatca cctccgcttg ggcgagccga
     2401 tcgcgttcca agtcgcgttc ggggtcgtga tctggctggg actcttcctc cgcgagccgc
     2461 gactgcgggc gattctgcca atccgctcgc caaacgcaac aaccacaaga ccgtagcctt
     2521 caattctcat tgcacttcat aaggaccatc ccatgtcttc cgtccgtgtt ctcatcggca
     2581 ctaagaaagg cgcgttcatt gccacagccg acggcaaacg ccagaagtgg accatcagca
     2641 cgccgcaatt cggcggctgg gagatgtatc acctcaaggg ctcgaccgtc gatccaaacc
     2701 ggatttatgc ctcgcagtcc agcggctggt tcgggcaagt catctcgcgc tccgacgacg
     2761 gcggaaaaac gtggaccacg cccggcagca agccggagga actgcaatcg ccggaaggca
     2821 tgcccaaagg cgagagcaac aagttcgtct ataacaccga gccaccaacc ggcaagccgc
     2881 tgaccacgca ccagtggtac gacggcactc agcacgcctg ggaattcaag cgcgtctggc
     2941 acctcgaacc ctcgctgacc gatccggaca ccgtgtatgc gggcgtcgag gacgcggccc
     3001 tcttccgctc gaccgacggc ggaaagacgt ggcaggaaat cgcctccctc cgcggccacg
     3061 ggaccggacc gaagtggtcg cccggcgccg gcggcatggg gctgcacacc atcgttcagg
     3121 acccgaagaa tccccagcgg atgtacattg caatctccgc agccggcgcc ttccgcaccg
     3181 acgacggcgg ccaatcgtgg aaggcaatta acaaagggct gaaatcgcct tatctgcccg
     3241 agatgacgcc cgaagtcggc ttttgcgtcc atcggatcgc gctccacccg tcgaatcccg
     3301 atgtgctgtt catgcagttg caccagggcg gcggcgtcta tcgcagcgac aacgcaggcg
     3361 accagtggca ggaggtcaac ggcaacttac cgaccgactt cggcttcccg atcgacgttc
     3421 acgcccacga gcccaagacc atttattcga ttccgatgga tccgtacctc cgcgtaccgc
     3481 ctgggggcaa gctccgtgtc tggcgcagca ccgccggcgg caacgagtgg gaaccgctca
     3541 ctaaggggct gccgcaggag aattgctacg tcaacgtcct gcgcgatgcg atgtgcgtcg
     3601 attcgctgga cgactgcggc atctatttcg gcacgaccgg cggacaggtt tattgctcgc
     3661 ccgacggcgg caacaactgg cacgccatcg cccgcgacct gccgtacgtc cagtcggtgg
     3721 aggtccagac gctgtcatga ttcgcgtcgt cattccttat catctccggc ttctttctgg
     3781 cgtgacgggc gaaatcgcgc tcgaagtcga gccgcccgtc acgcagcgcg ccgtgctaga
     3841 cgccctggaa gctcgctatc cgatgctcaa ggggaccatt cgcgaccacg tcacgttcaa
     3901 gcgccgcgcg ttcctgcgct actacgcctg cgagcaggat ctttccctcg aagagcccga
     3961 tgcacaactt cctgccgcag ttgcgtcggg caaagagccg ttcctggtca tcggcgccat
     4021 cgccgggggt tagtgctcgg ctttcgcgat gtgtgataca ctccgctatc cgcaacgtat
     4081 tctggcggta acggaatcgg atgacggcac aggaaaacgc cactgacgcg ctggctgcct
     4141 cggcttctcc atctgccgat gcgccgatcc accccaacca cttccctctc cgtctgatcg
     4201 ttgccggaat cgtcggcaat gtgatggaat ggtacgactt tgcggtgtac ggctattttg
     4261 ccacatcgat cgggcagcat ttctttccgt ccgacaatcc atcgacgtct ttgatttccg
     4321 cgttcggcgc atttgccgcg gggttcctga tgaggcccct gggcggtctc gtgttcggcc
     4381 acatcggcga ccacttcggc cgcaaggcgg cactgacact ctctgtgctg gcgatggcca
     4441 tcccgacgtt ccttatcggc gtgctgccgg ggcatcaggt cttgggaacg ggatccgcgg
     4501 tgttactggt ggttctgcgg atgatccagg ggctctccgt cggcggagag tacacaacgt
     4561 cgatcgtgtt cctctttgag ggtgcggggc aaaaacacca gggcttcgcc tcgagctgga
     4621 gcggcttcgg tgccgtcggc gggattttgc tcggctccgc ggtgggtgcc gtggtgacag
     4681 gtctgctgcc ggaagagacc gttcacaact gggcctggcg actgccgttc ctggtggggc
     4741 tggcggtcgg cctggcgggg ctctacgtcc gcgggcacat tcccgaacct ccccgtcagc
     4801 cgcgggagaa aatgggacgt tcgcccgtct gggacgcctt gcagaccgag tggcgcacga
     4861 ttttgcgtct ggccctgctc aacacggtca atggcatcgg tttctacatg gcgtttgtgt
     4921 ttctcgtcac ctacatgaag tcggtgggag ggctgaccga ggccatggcg ctggagatca
     4981 atacggtcaa catggccgtg ctgctggcga tgtttccgct ggcaggctgg ttgtcggacc
     5041 gcatcggccg cataccgctg ctcgtcgtgg gcatcggcgg catgctcctg ttttcgtggc
     5101 cgctcttctg ggccttgcac catccgtcag ccgcctggag cttcgtcggg caactcggct
     5161 tcgccgtctg catcggcctg tttggcggcg tgatcccggt ggccatggtg gaaatgacgc
     5221 ctgccgcggt gcggtgcagc gccatttcga tcggctacaa cttgtgcgtc ggggtcctcg
     5281 gcggaacgac gcccatggtg gcggcctggt tgatgaagga gacgcgcgac gtcctggccc
     5341 ccgcctggta tctcatggtc gccgccgcaa tctccctcgg cgtcgtcgta gtcccgctgc
     5401 tattcagccg caaacacacc gccaccaaat cctgcaacga gtcggggtga taggatttga
     5461 acctacgacc tcttggtccc aaaccaagcg cactagccag gctgtgctac accccggttt
     5521 ttgtcagttg tcattggtca gtgggcagtt gtcaatcgcg ggtcgcgcgc gattggcact
     5581 ggatcagtcc caagtagcag gtgatcgcag ctcaggcgga cttgcggcgg gtagcaggcg
     5641 gtgcagcatc ggtgagttgc tcgcggaggt ggtcgaattc gtcgtggttc tggaagtgga
     5701 tgacgagctt gccgcggccg cggtggttgg cccgaagttc gaccttgagg cccagggcgt
     5761 ggcggatctc ctgctccaga gacgcgacgt gctcgctgcg ggttcgggcc ttggagccgg
     5821 ggccgggcag gatttccaca tcctcgccgt cttcgtcgcc gacttgctcg ccgacgatac
     5881 gttccacgtc gcggacgctg aggccctcgg cttcgatacg cttggcaagc gcgacctgct
     5941 cgtgctcgtc gcccaggggc agcagggctc gggcgtggcc ggcggtgatc gagcctgtgc
     6001 tgagcccgct caggatcgac ggcggtaact ccaagagccg catcaggtta gcgatcgtgg
     6061 aacggtcgat tttcaggcgt ttcgccaggt cgtcctgcgt gcagcggtgc tcgtcgagat
     6121 aacgcttgaa cgagagggct ttttcgaccg ggttcaggtc cttacgctgc aggttctcga
     6181 cgatggccag ctcggcgacc aggcggtcgt cggcgtcgcg gactcgggcg ggaatcgtcg
     6241 tccagccggc gcgtttggcg gcacgcagac gcc
//
LOCUS       DASUGB010000970         4612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_664357, whole genome shotgun sequence.
ACCESSION   DASUGB010000970 DASUGB010000000
VERSION     DASUGB010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664357"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VFV87_18380"
     CDS             <1..1004
                     /locus_tag="VFV87_18380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5105794.1"
                     /translation="EEWKNVLKELRSLKVKYQSAPEAEQAKLQDEWNTQIVKGNALLG
                     SLQAAALKAYEESPNTDPQLTRFLVKLASDQIEHDRYTDAQEITTVLIANECPDKSIY
                     DSAAIAAYVLNDFDKAEEYFKAAQDAGALSPYGQELVNTVSDYKELWKEEKKLREEEA
                     KKDDLPRVKLETTQGDIIVELFEDQAPDTVGNFISLVEGQDGKGFYDGIAFHRVLKNF
                     MAQGGDPKGDGTGGPGYNIYCECYNEPFRRHFAGTLSMAHAGRDTGGSQFFLTFRPTP
                     HLNGKHTAFGRVIEGLDVLPKLTRRNPDDAAAPEPDKIIKATVLRKRDHAYAPKKVQ"
     gene            1119..2267
                     /locus_tag="VFV87_18385"
     CDS             1119..2267
                     /locus_tag="VFV87_18385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5105795.1"
                     /translation="MRVLLPLALLALLAVATAPLLAQKPDEPKPAETPAPPAAEPLSP
                     AQQFQAAHREWTALDKRLTELQQMYAKETAAAARAEIKKQYQELVDQSELLLPKLQAA
                     AIAAYEAEPNKDAEVTRTLIGLVAYAYRSDDYETALKLARLMEAQNCDEAVLYGLAGA
                     AAFQSDDYETAEAYLTRADKAGKLDTEGKELLAALPQQKKAWAKEQEIRKAEATADDL
                     PRVKLETSKGPIVIELFENEAPQAVGNFVSLVESKFYDGLTFHRVLPGFMAQGGDPTG
                     DGTGGPGYEIYCECYKENARQHFRGTLSMAHAGRDTGGSQFFLTFGPTTHLNGKHTAF
                     GRVIEGLDVLAKLQRRDPTRANPPPPDKIVKAEVLRKRDHKYEPTKVK"
     gene            2326..3093
                     /locus_tag="VFV87_18390"
     CDS             2326..3093
                     /locus_tag="VFV87_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5105796.1"
                     /translation="MQVADRTFVITGGASGLGAGCVRRLAGLGASIVIADLNAAAGEA
                     LAKELGNRVAFVFTDVTSAESGQAAVDTAIKQFGGLHGLVNCAGILGASRIVGREGPH
                     DLALFQKVIAVNLVGTFNMLRLAAAAMTNNEPDAEGERGIIVSTSSVAAYEGQLGQAA
                     YAASKGGVASLTLPAARELAKFGIRVVAIAPGIFDTAMMQAAPDAVRDSLTEQCVFPP
                     RFGRAGEFAQLVQHILENPMLNGCILRLDGAVRMGAK"
     gene            complement(3103..>4612)
                     /locus_tag="VFV87_18395"
     CDS             complement(3103..>4612)
                     /locus_tag="VFV87_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEX5105797.1"
                     /translation="AENPATREPLEEYPVSTWQDCEEALSAAAGAFQQGRAISAEKLA
                     AFLEAFAGRIEARKDEIVELASAETALPISPRLADNELPRTTGQLRQAAAAAREGSWA
                     QPTIDTKNNIRSMLAAVGPVVVFGPNNFPLAFNSASGGDFAAAIATGNPVIAKANTSH
                     PGTTRLLAEEAHAAAEAAGLPPGWVQLLYRTSHEDGERLVADHRIGATGYTGSRAAGL
                     KLKAAADKAGKPIYLELSSVNPVVILPGALDEKGDDIAGQFATSCLMGTGQFCTNPGL
                     VILLAGERTEQFIDQLVEKFKAAPVGTLLSAGVAKSLESGISFLIRAGAHTLVGGKHA
                     AGKGYCHANTLLRASGRQFLEAPEAMQTEAFGNASLLVVAADLDEACRVGEQLEGNLT
                     GCVYSHGGGADDAAYERLAPILRQKVGRLLNDKMPTGVAVSPAMNHGGPYPATGHPGF
                     TAVGIPAALRRFAALACYDGVRDHRLPAALQNKNPSGQMWRLIDGKWTTGDV"
BASE COUNT          866 a         1572 c         1434 g          740 t
ORIGIN      
        1 tcgaagagtg gaaaaacgtc ctcaaagagc tgcgctcctt gaaggtcaag taccaatccg
       61 cccccgaagc cgagcaggcc aagctgcagg acgagtggaa tacgcaaatc gtcaagggga
      121 acgccctcct gggcagcctg caagctgctg cattgaaggc ctacgaggaa tcgcccaaca
      181 ccgatccgca gctcacgcgc tttctcgtca agcttgccag cgaccagatc gagcacgacc
      241 gctacaccga cgcccaagag attacgaccg tcctgattgc caatgagtgc cccgacaagt
      301 cgatctacga ctcggctgcc attgccgcct atgtcctgaa tgacttcgac aaagccgagg
      361 agtatttcaa ggcagcccag gacgcgggcg cgctgtcgcc gtacggccag gagctggtga
      421 atacggtcag cgattataaa gagctgtgga aagaggagaa aaaactccgc gaagaggaag
      481 caaagaagga cgatctgccg cgcgtgaaac tggaaaccac gcaaggcgac attatcgtcg
      541 agctgttcga ggaccaggcg cccgataccg tcggcaattt catcagcctc gtggaagggc
      601 aggacggcaa aggcttctac gacgggatcg cgtttcaccg cgtgctgaag aacttcatgg
      661 cccaaggggg cgacccgaaa ggcgacggca cgggcgggcc gggttacaac atctactgcg
      721 aatgctacaa cgagcctttt cgccgccact tcgccggcac gcttagcatg gcccatgccg
      781 gccgcgatac gggcggctcg cagttcttcc tcacattccg gccgacgccg cacttgaacg
      841 gcaagcacac ggcttttggg cgcgtgatcg aaggcctgga cgtgctgccg aagctcaccc
      901 gccgcaatcc cgatgacgcc gccgctccgg aaccggacaa gatcattaag gccacggtcc
      961 tgcgcaagcg ggaccatgcg tatgcgccga agaaagtcca ataagcggcc tggcggtgga
     1021 agaaccagga actgaagaat caagtaccca tctcgttccc acgctctgcg tgggaacgca
     1081 caccccgacg ctccgcgtcc tcgaatgtga gtccttccat gcgagtactc ctccctctcg
     1141 cactgctcgc cctgctggct gtggcgactg ctcctctcct ggcgcagaaa ccggacgagc
     1201 ccaagccggc ggaaacaccc gcgccgcccg ccgccgagcc gctgtccccg gcccagcaat
     1261 tccaggcggc ccatcgcgaa tggacggcgc tcgacaagcg gctcaccgag ctgcagcaga
     1321 tgtacgccaa ggaaaccgct gcggcggccc gggcggagat caagaagcaa taccaggagc
     1381 tggtcgatca aagcgagctt ttgctgccga agctgcaggc cgccgcgatc gccgcctatg
     1441 aggccgagcc gaacaaggac gccgaggtca cccgcacgtt gatcggcctg gtcgcctatg
     1501 cctatcgcag cgacgactat gaaactgccc tcaagctggc ccgcctgatg gaagcccaga
     1561 actgcgacga ggcagtgctc tatggtctgg cgggcgcggc ggcgttccag tcggacgatt
     1621 acgaaacggc agaggcgtac ctgacgcggg ccgacaaggc cggcaagctc gacacggaag
     1681 gcaaggagct gctcgccgcc ctcccccagc agaaaaaggc ctgggccaag gaacaggaaa
     1741 tccgcaaggc cgaagccacg gccgacgacc tgccgcgcgt caagctggaa acgagcaagg
     1801 ggccgatcgt gatcgagctg ttcgagaacg aagcgccgca ggcggtcggc aacttcgtca
     1861 gcctggtcga gagcaagttc tatgacggcc tgacctttca ccgcgtcctg ccgggattca
     1921 tggctcaggg cggcgaccca acaggcgacg gcacgggcgg tccgggctac gaaatctact
     1981 gcgaatgcta caaggagaac gcccgccagc atttccgcgg cacgctcagc atggcccacg
     2041 ccggccgcga taccggcggc tcgcagttct ttctcacctt cggccccacg acgcatttga
     2101 atggcaagca cacggctttc ggccgcgtaa tcgaggggct ggacgtgctc gccaagctgc
     2161 agcgccgcga tcccacgcga gccaatccgc cgccgcccga taagatcgtc aaagccgaag
     2221 ttctgcggaa gcgcgatcac aagtacgagc cgacgaaggt gaaatagccg taggacgggt
     2281 ttcccaaccc gtccgtgaag ctgcgagaca ggggacggga cacgtatgca ggtcgctgac
     2341 cgcactttcg tgatcaccgg cggcgcgtcc ggtctcggcg ccggttgtgt gcggcggttg
     2401 gctggtttgg gcgcgagcat cgtgattgcg gatctgaatg ccgccgcagg cgaggcgctt
     2461 gccaaggagc tcggaaaccg cgtcgcgttc gttttcaccg atgtcacctc tgccgaaagc
     2521 ggccaggccg cggtggatac ggcgatcaag cagttcggcg gtctgcacgg gctggtcaat
     2581 tgtgcgggca tcctcggcgc gagccggatc gtcggccgcg aaggcccgca tgacctggcg
     2641 ctcttccaaa aggtgattgc cgtcaacctg gtcggcacgt tcaacatgct ccgcctcgcg
     2701 gcagccgcga tgacgaacaa cgagccggac gcggaaggtg agcggggcat catcgtcagc
     2761 acctcatcgg tcgcggccta cgaaggacag ctcggccagg cggcttatgc ggcatcgaag
     2821 ggcggcgtcg cttcgctcac gctgccggcg gctcgcgaac tggcgaagtt cggcatccgc
     2881 gtcgtcgcga tcgcgccggg catcttcgat acggccatga tgcaggccgc tcccgatgcc
     2941 gtccgcgatt ctctgaccga gcagtgcgtc ttcccgccgc gcttcggccg cgcgggcgag
     3001 ttcgctcagc tcgtgcagca catcctcgaa aatcccatgc tcaacggctg catcctgcga
     3061 ctcgacggcg cggtgcggat gggtgccaag tagccggcta catcacacat cccccgtcgt
     3121 ccacttccca tcaatcagcc gccacatctg gccgctgggg ttcttattct gcagcgccgc
     3181 cggcagccgg tggtcgcgga cgccgtcgta gcacgccagc gcggcgaagc ggcgcagggc
     3241 ggcgggaatc ccgaccgccg taaagccggg atgacccgtc gccggatacg gcccgccgtg
     3301 attcatcgcc gggctgacgg ccacacccgt cggcatcttg tcgttcaaga gccggccgac
     3361 cttctgccgc aagatcggcg cgagccgctc ataggccgcg tcatccgccc cgccgccgtg
     3421 cgaataaacg cagccggtca ggttcccttc cagctgctcg ccgacgcggc aggcttcgtc
     3481 gagatcggct gcgacgacga gcagcgacgc attgccgaat gcctcggtct gcatcgcctc
     3541 gggcgcttcg aggaactgcc gcccgctggc ccgcagcagc gtgttcgcgt ggcaatagcc
     3601 cttacccgcc gcgtgcttgc cgccgaccag cgtgtgcgcc cccgcgcgga tcaaaaagct
     3661 gatgcccgat tccagcgact tcgccacgcc cgccgagagc agcgtgccga ccggcgcggc
     3721 cttgaatttc tcgaccagtt ggtcgatgaa ctgctccgtc ctctcgcccg ccagcaggat
     3781 gaccagcccc ggattcgtgc aaaactggcc cgttcccatc aggcagctcg tcgcgaactg
     3841 accggcgatg tcgtccccct tttcatcgag cgcgcccggc agaatcacca ccggattgac
     3901 gctcgatagc tccaggtaaa tcggcttgcc ggctttgtcg gcggctgctt tcagtttcag
     3961 tccggctgct cggctgccgg tgtagccggt cgcgccgatg cgatggtccg caaccagccg
     4021 ctcgccgtct tcgtggctcg tgcgataaag gagctgaacc cagcccggcg gcaggccggc
     4081 cgcttccgcc gcggcgtggg cttcctcggc cagcagccgc gtcgtgccgg ggtgcgacgt
     4141 gttcgccttg gcgatcaccg ggttgcccgt cgcaatcgcc gcggcaaaat cgccgccgct
     4201 ggcgctgttg aaggccaacg gaaaattatt cggcccgaag acgacgaccg ggcctacggc
     4261 cgcgagcatc gaccggatgt tgttcttcgt atcgattgtc ggctgcgccc acgagccctc
     4321 gcgggccgcc gcggctgcct ggcgaagctg ccccgtcgtc cgcggcaatt cgttatccgc
     4381 cagccggggc gaaatcggca gcgccgtctc cgcgctggcc agctcgacga tctcgtcctt
     4441 gcgggcctcg atccgcccgg caaacgcctc cagaaacgcc gccagcttct ccgccgaaat
     4501 tgccctgcct tgctgaaacg ctcccgccgc cgcgctcaag gcctcctcgc aatcctgcca
     4561 cgtgctgacc ggatactcct ccagcggctc ccgcgtcgcc ggattttccg cc
//
LOCUS       DASUGB010000971         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_527112, whole genome shotgun sequence.
ACCESSION   DASUGB010000971 DASUGB010000000
VERSION     DASUGB010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527112"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFV87_18400"
     CDS             <1..1080
                     /locus_tag="VFV87_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5105798.1"
                     /translation="QGINRWFGYYCQGVAHNHYPTYLWSDDERYPLDNPAFSAHQKFP
                     ADAEPTDPAAYKRYEGKHFAMDLITEQAVKFVQDNKDKPFFLYYPTIIPHMAMQVPAD
                     SLAEYKDAFPEKPYLGEGSYLPHQTPHAAYAAMITRMDRHVGQIIKAVQDAGLDERTI
                     FIFSSDNGPAYSYGGTDDAFFNSAGGLRGRKGSLYEGGIRVPTVVRWKGHVAAGTSSD
                     FVSGLEDWLPTLLALAGASDSAPKDVDGIDISPTLLGRSQPPRPFLYREFPAYGGQQS
                     VRVGDWKGIRQNLLPAGGGGKAKKAKVAREPDMHIELYNLKTDPHEEKDVSAEQPAVV
                     TLMARLMLTQHAPSKEFPFPALDGQ"
     gene            complement(1097..1495)
                     /locus_tag="VFV87_18405"
     CDS             complement(1097..1495)
                     /locus_tag="VFV87_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5105799.1"
                     /translation="MEAENPQLLQLAEQWANTVLIWVGFGTIVGLLAKAIMPGKDPGG
                     AVATLAMGIAGTIIGCAVLSYFWQGHLVSPISPLGFCVATFGAFVLLFFYKLLGGYYF
                     KEDQGPLVARRRVGFRRSRQPATVVYDDEH"
     gene            complement(1640..1846)
                     /locus_tag="VFV87_18410"
     CDS             complement(1640..1846)
                     /locus_tag="VFV87_18410"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX5105800.1"
                     /translation="MNEPASSAAASSPPAATLAPSPAIAAADSRESWRSEELLGDKTE
                     VQILHGTEVYRLRRTRQGKLILYK"
     gene            complement(1969..>3171)
                     /locus_tag="VFV87_18415"
     CDS             complement(1969..>3171)
                     /locus_tag="VFV87_18415"
                     /inference="COORDINATES: protein motif:HMM:NF023294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HEX5105801.1"
                     /translation="LDKIAATNARSRTADGNSAALKLTKHVEGNGGAWIASVSADTKA
                     FSASIKYGVLTRREQTFLLNYYANYLDASAGEFNALARDNVLPLDIVPEKNQKGVTLQ
                     VLFNGNPAVGSEVVIFDPTAQEIEAKTDEAGKVELETAKPGLYSIRAKWVVMQAGKEA
                     DQEYPQVNHYSTLALRVSGGEAAAKTTATAKQLTAAQLLHAAREGRAVWDDFPGFEAD
                     LKIFAEGREQTGRITITADGGVSLSGIELKDEKTVLAMLRSLIGHRMPGGESDDNVSF
                     ADEQDDHPLGRLIKLDYDSAMASAYRIKDGVIREVNRQMDGGRFTISVFEVQRNPEGK
                     YLPGYYTVSFWNKDGALRNTTVTHETWTRVGRFDLPATHASVATGKDDHRNTSLIFSN
                     HKLLERQN"
BASE COUNT          594 a          929 c         1045 g          603 t
ORIGIN      
        1 cagggcatca atcgctggtt cggctattac tgccagggcg tggcccacaa ccattatccg
       61 acctatctgt ggagcgacga cgagcgatat ccgctcgaca acccggcgtt ttcggcacat
      121 cagaagtttc ctgcggacgc cgaaccgact gatccggccg cttacaagcg ctacgaaggc
      181 aagcacttcg cgatggacct gattaccgag caggccgtca agttcgttca ggacaacaaa
      241 gacaagccgt tcttccttta ttacccgacg atcatcccgc acatggcgat gcaagttccc
      301 gcagattcac tggcggaata caaggacgcc tttcccgaga agccgtacct gggcgagggg
      361 agctatctgc ctcaccagac gccgcacgcg gcgtatgccg cgatgatcac gcggatggac
      421 cgacacgtag ggcagatcat caaggcggtg caagatgcag ggctcgatga gcggacgatc
      481 ttcatcttct ccagcgacaa cggcccggct tacagctatg gcgggacgga tgacgcgttt
      541 ttcaattcgg ccggcgggct gcgcgggcga aagggctcgc tctacgaagg agggattcgc
      601 gtgccaacgg tagtccggtg gaaaggacac gtcgctgctg gcacgtcgag cgatttcgtg
      661 agcggcctgg aagattggct gccgacgctg ctggctttgg ctggggccag cgacagtgcg
      721 ccgaaagacg tggacggcat cgacatttcg ccgacgctcc taggccggtc gcagccgccg
      781 cggccgttcc tctatcggga attccccgct tatggcggcc agcaatcggt gcgcgtcggc
      841 gattggaagg gcatccgcca gaacctgctg cccgctggcg gcggcggtaa agccaagaag
      901 gcgaaagtgg cccgcgagcc tgacatgcac atcgagttgt acaacctcaa gaccgatccg
      961 cacgaagaaa aggacgtctc cgccgagcag cccgcggtcg tgaccctgat ggcccggcta
     1021 atgctcacgc agcacgcgcc gagcaaagaa ttccccttcc cggcgctgga cggtcaatag
     1081 ccgaaggatg ccgcattcaa tgctcgtcgt catagacgac ggtcgccggc tggcgggagc
     1141 ggcgaaagcc gacgcgccgg cgagcgacga gcgggccttg atcttccttg aagtagtagc
     1201 cgccgaggag cttgtagaag aacaggagca cgaacgcgcc gaacgtggcg acgcaaaagc
     1261 caagcgggct gatgggcgag acgagatgcc cttgccagaa gtaagagagg accgcgcagc
     1321 cgatgatcgt gccggcgatt cccatcgcga gcgtggcgac ggctccgcca gggtccttgc
     1381 cgggcatgat tgccttggcg agcaggccca cgatcgtgcc gaagccgacc cagatcagga
     1441 ccgtattggc ccactgctcg gcgagctgga ggagttgcgg gttttcggct tccatggggc
     1501 cctcctgtgc aacggacatc cttgaaggaa tcgaccagga gcgtgcggca acttcggtcc
     1561 ggttgtgcag aagggagccg gcttggcgca tgggcgattt gaagctgttg ctagcgagac
     1621 gcaagcgtta gcacgctagc tacttgtaga gaatcagctt gccctggcgt gtgcgacgga
     1681 ggcgataaac ctcggtgccg tggaggattt gcacttcggt tttgtcgccg agcagttcct
     1741 ccgatcgcca cgactcgcgc gagtcggccg cagcaattgc gggtgaaggc gccagcgtgg
     1801 cggcgggcgg gctggaagca gcagcggatg acgcgggctc gttcatgatc gggagccgga
     1861 gttgccgcgg acccggctgg cagggagcgg agaagcgcct gccagccgaa gccgcgactg
     1921 cacggttggt tatgttggcc tgcggcgact cgacgcacgc caaatgagtt agttttgcct
     1981 ttcgagcaac ttgtggttgc tgaagatcag cgacgtgttg cgatggtcgt ccttgccagt
     2041 ggcgaccgaa gcgtgagtcg ccggcaggtc gaaccggccg acgcgggtcc acgtctcgtg
     2101 tgtcacggtt gtattgcgga gcgcgccgtc tttgttccag aaactgacgg tgtagtagcc
     2161 ggggaggtac ttgccctcgg gattgcgctg gacctcaaag acggagatgg tgaagcggcc
     2221 gccatccatc tggcggttga cttcgcggat gacgccgtcc ttgatgcgat aggcgctggc
     2281 catcgccgag tcgtagtcga gtttgatcag ccggcccagc ggatgatcgt cctgctcgtc
     2341 ggcgaaggag acattgtcgt ccgactcgcc gccgggcatt cgatggccga tcagcgaccg
     2401 cagcattgcc aggactgtct tctcgtcctt cagctcgatg ccgctgaggg aaacgccgcc
     2461 atcggcggtg attgtgattc tgccggtctg ctcgcggcct tcggcgaaga tcttcaggtc
     2521 ggcttcaaag ccgggaaagt cgtcccacac ggcgcggcct tcacgggcgg cgtgtagcaa
     2581 ctgcgctgca gtgagctgct tggcggttgc agttgtcttg gctgctgcct cgcccccaga
     2641 gacccgcagg gcgagcgtgc tgtagtgatt cacctgtgga tactcctggt ccgcctcctt
     2701 gccggcttgc atcaccaccc acttggcgcg aatcgagtag agaccgggtt tagcggtctc
     2761 tagctcgacc ttgccggctt cgtcggtctt ggcttcgatc tcctgagcgg tcgggtcgaa
     2821 aatcacgacc tcgctgccga ctgccgggtt gccattgaac agcacctgga gcgtgactcc
     2881 cttctggttt ttttcgggaa cgatatccag tggcaaaacg ttgtcgcggg ccagagcatt
     2941 gaattcgcca gcagaagcgt cgagataatt ggcgtagtaa ttcaacagaa acgtctgctc
     3001 gcggcgggtg aggacgccgt atttgatcga ggcgctgaag gccttcgtgt cggcggaaac
     3061 cgaggcgatc cacgcgccgc cgttgccttc aacgtgcttg gtcagcttca aggccgccga
     3121 attgccatca gccgtgcggc tccgggcgtt ggttgcagcg atcttgtcaa g
//
LOCUS       DASUGB010000972         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1777261, whole genome shotgun sequence.
ACCESSION   DASUGB010000972 DASUGB010000000
VERSION     DASUGB010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777261"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFV87_18420"
     CDS             <1..416
                     /locus_tag="VFV87_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5105802.1"
                     /translation="VGDWTTDEGRKFLESRSPLFFVEKIKRPLLIGQGANDPRVKQAE
                     ADQIAKAMSEKKIPVTYVLFHDEGHGFARPENRFAFYAITEAFLAENLGGRFEPIGSA
                     FEGADFSVPNGKDQVPGLSEGLKAMKTGTTGAARE"
     gene            452..694
                     /locus_tag="VFV87_18425"
     CDS             452..694
                     /locus_tag="VFV87_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX5105803.1"
                     /translation="MNSTMIPVTAMDELLSLSAPQKLALIGALWDSIEAEGQPVPVSD
                     ALVAELDRRKANAAKNPDSLVPLEAILKRLGRTDGA"
     gene            684..989
                     /locus_tag="VFV87_18430"
     CDS             684..989
                     /locus_tag="VFV87_18430"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5105804.1"
                     /translation="MAHKAPVRFVPEADAEFEGSRAWYEGRRAGLGEEFVDAGLACLD
                     RIRESPKLYARYKEEYRRASVRRFPYGIYYEFENGVVVVYAVFHESQDPVKLDRRLL"
     gene            1050..1556
                     /locus_tag="VFV87_18435"
     CDS             1050..1556
                     /locus_tag="VFV87_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105805.1"
                     /translation="MSPPNFEMLTLAQFPQGNGPGGWICLAFAVFAGLAIAIRIVAGA
                     FDQSRIAEYIQSRGGRVVSVQWSPFGRGWFGEKNDRIYEVVYYDADGLQHFATCKTSL
                     FSGVYWTEDRVSHAKPQWFDKANATSQEPFIQGIEFLDDGDLRDENRRLKEEVARLKS
                     ELEGRDRI"
     gene            1587..2681
                     /locus_tag="VFV87_18440"
     CDS             1587..2681
                     /locus_tag="VFV87_18440"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5105806.1"
                     /translation="MHEISAATVADYLRTSGRATAAEPIEARELSGGVSNVVLLVELS
                     SRGERFVVKQARGKLRVQEEWLCPIERIWREVETLQICGELLKALPQSAIRNPQSAMM
                     SPPSIVAAFQSGVPSVLWEDRTNYCFAMTAAPAGHKTWKELLLAGELPLSLGIATGCG
                     EMLAALHAGSWGNAEIAARLDDRQFFDQLRIDPYYRHVAKVHPDLAPQLQQLIDSVWS
                     HRRCLVHGDFSPKNLLVWQGQTMLIDFEVGHYGDPAFDLGFFLTHLVLKAIWSGARRE
                     RYLGLATAFWNTYRLRMEQSVSPAEIDDLESRAMFNLAGCLLARIDGKSPVEYLGDTQ
                     RQAVRERGRQWLMRPPSTWTDTADSLSPEY"
     gene            complement(2686..>3771)
                     /locus_tag="VFV87_18445"
     CDS             complement(2686..>3771)
                     /locus_tag="VFV87_18445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5105807.1"
                     /translation="RLRKDLSAARAHLVVEQAELRERARDKFSAAEQMFFTRKGLEQA
                     TDEQIATYKASRFPAGAIADLCCGIGGDTISLLGNGPIAAVDVDPIACLLTEANAAEC
                     KSSFNARTENADAVTFPVGEFSTWHIDPDRRPEGRRTSRVELFQPPLVVIQSHLASNP
                     HGAIKVAAAAELPAAWRETAELQWLGSRGECRQQVAWFGSLARHSGQRSAMIVDAPGG
                     PRTIVGTGDDSIPLATRLGRYLFVPDNAVLAARLTGALCRELGLAAIAPGIAYLTADA
                     FVSDGALAAFEVQDVLPFDRKQLKAWCRERHIGRLEVKKRGIDVSPESLRKAVIGTGD
                     REATLVVTRLESRAAAIVVRRISATDS"
BASE COUNT          726 a         1144 c         1211 g          690 t
ORIGIN      
        1 gggtcggcga ctggacgacc gatgaaggtc gcaagttcct cgaatcgcgc tcgccgctgt
       61 tcttcgtcga gaaaatcaaa cggccgctgt taatcggcca aggggcgaac gacccgcgcg
      121 tcaagcaagc cgaagccgac cagatcgcca aggcgatgag cgagaaaaaa attccggtga
      181 cgtacgtgct gttccacgac gaggggcacg gctttgcgcg gccggagaac cgcttcgcgt
      241 tctacgcgat caccgaagcc ttcctcgccg agaacctcgg cggccgtttc gagcccatcg
      301 gcagcgcatt cgaaggcgcc gatttcagcg tgcccaacgg caaagaccag gtgccgggat
      361 tgtcggaggg gctgaaggcc atgaaaaccg gcacaacggg cgcggcgagg gagtagaatg
      421 gttgagtgcc gacacccagg agaccgaatc gatgaattcg acgatgattc cagtgactgc
      481 gatggacgaa ttgttgtccc tcagcgcgcc gcaaaagctg gccctgatcg gtgcattgtg
      541 ggacagcatc gaagcggagg gccagcccgt tcccgtgtcc gatgctctcg tcgccgagtt
      601 ggatcgaaga aaggccaacg cagcgaagaa tcccgattcg cttgtcccct tggaagccat
      661 tttgaaacga ctggggcgca cggatggcgc ataaggctcc ggttagattc gttcccgagg
      721 cggatgcaga atttgaaggt tcccgggctt ggtatgaggg ccgtcgcgca ggcctaggcg
      781 aggaattcgt cgatgctggg ttggcgtgtc tggaccgcat tcgcgaatcg cccaagctat
      841 atgcacgcta caaggaggaa taccgccggg cctccgtacg gcgctttccg tacgggatct
      901 attacgagtt tgaaaacggc gttgtagtcg tctatgccgt ctttcatgaa tcgcaggatc
      961 cagtgaagtt ggatcggcga ttgctctaga ggaaaggctt ccacccacct agcgcagcgg
     1021 cgaccaggcg acgcctgagc tcgatgatga tgtcaccgcc gaactttgaa atgctgacgc
     1081 tggcgcaatt cccgcaaggg aatggcccgg gtggctggat ttgcctggcg ttcgccgtgt
     1141 ttgcgggcct ggcgatcgcc atccgaatcg tagcaggcgc gttcgaccag tcgcggattg
     1201 ccgagtacat ccaaagccgc ggcggtcgcg tggtgagcgt gcaatggtcg ccgtttggca
     1261 gaggctggtt cggcgagaag aacgaccgca tttacgaagt cgtgtactac gacgcggatg
     1321 gcctgcagca ctttgccaca tgcaagacgt cgctgttttc cggcgtgtat tggacggagg
     1381 atcgggtctc gcatgccaaa ccgcaatggt tcgacaaggc gaatgccacg agccaagagc
     1441 cgttcattca ggggatcgag tttttggacg acggggacct gcgcgacgag aatcgccgcc
     1501 tcaaggagga agtcgcccgg ctgaagagcg agctcgaagg ccgcgatcgc atctagctgg
     1561 tccgcgacac gctgccaggt gcttccatgc atgaaatcag tgctgcgact gttgccgatt
     1621 acctgcggac ctccggccgg gcaacagccg ctgagccaat cgaggcgcgc gaactttccg
     1681 gcggcgtgtc gaatgtggtg ctgctggtcg aactgtccag ccgcggcgag cggttcgtcg
     1741 tcaagcaagc ccgcggcaag ctgcgcgtgc aagaggagtg gctctgcccg atcgaacgga
     1801 tctggcgcga agtcgaaaca ctgcaaatct gcggcgaatt gctcaaagcg ctcccccaat
     1861 ccgcaatccg caatccgcaa tccgcaatga tgtccccgcc gtccatcgtc gccgccttcc
     1921 agtcaggcgt tccctcagtg ctgtgggaag accgcacgaa ctattgcttc gcgatgacgg
     1981 ccgccccggc cggtcacaag acgtggaagg agctgctctt ggcgggcgaa ctgccgctga
     2041 gcctgggcat cgcgaccggc tgcggcgaga tgctcgccgc gctgcacgcc ggctcgtggg
     2101 gaaatgcaga gatcgctgcc cgactggatg accggcagtt tttcgatcag ctccggatcg
     2161 atccctatta ccggcacgtg gccaaagtcc atcccgatct ggcgccgcaa cttcagcagc
     2221 tcatcgactc cgtctggtcg caccgccgct gcctcgttca tggcgatttc agccccaaga
     2281 acctgctcgt ctggcaaggg cagacaatgc tgatcgattt cgaagtgggc cactacggcg
     2341 acccggcgtt tgatctgggg tttttcctga cgcacctggt gctcaaggcg atctggtcgg
     2401 gagcgcgccg cgagcgctac ctcggactcg ccacggcatt ctggaacacg tatcggctgc
     2461 gaatggaaca gagcgtttcg cctgccgaga tagacgactt ggaatcccgc gcgatgttca
     2521 acctcgccgg ctgcctgctc gcgcggatcg atgggaagag cccggtggag tacttgggtg
     2581 acacgcaaag gcaggcggtg cgcgagcggg gacggcaatg gttgatgcgg cctccgagca
     2641 cttggaccga caccgcggat tcattgagtc ctgagtactg agtactcagc tatccgtggc
     2701 cgagattcgc ctgacaacga tcgccgcagc tcgactttcc agtcgtgtaa caaccaaggt
     2761 tgcctcacga tcgcccgttc cgatcacggc cttccgcagc gattccggac tgacatcgat
     2821 gccccgtttt ttgacctcca aacgaccaat gtgtcgctcg cggcaccagg ctttgagctg
     2881 cttgcggtcg aagggcagca cgtcctggac ttcgaacgcg gctagggcgc cgtcgctcac
     2941 gaaagcatcg gcagtcagat acgcgatgcc cggcgcgatt gcagccaagc caagttcgcg
     3001 gcagagtgcg ccggtgaggc gagcggccag cacggcgttg tccggcacaa acagataccg
     3061 gccgagccga gtggcgagcg ggatcgaatc gtcgcccgtg ccgacgatcg tccgtgggcc
     3121 gccgggcgca tcgacgatca tcgcactgcg ttgccccgaa tgcctcgcca gtgagccgaa
     3181 ccacgcgact tgctgccggc attccccgcg gctgccgagc cattgcaact cggccgtttc
     3241 gcgccaggcc gcgggaagct cggcggcggc ggcgactttg atcgcgccgt gcggattgga
     3301 agccaggtgc gactggatca ccacgagcgg cggctggaag agttcgacgc gcgacgtccg
     3361 ccggccttcc gggcggcggt cgggatcgat gtgccaggtc gagaattcgc cgacggggaa
     3421 cgtaaccgcg tccgcgttct cggttcgcgc attgaacgag cttttgcact cagcggcgtt
     3481 cgcttccgtc aacagacagg cgatcggatc gacatccacg gcagcgatgg gtccattgcc
     3541 aaggagcgaa atagtatcgc ccccaatccc gcagcagaga tcggcgatcg ctccggctgg
     3601 aaaccggctg gccttgtacg tcgcgatttg ctcgtcggtc gcttgctcga ggcccttgcg
     3661 ggtgaagaac atctgctccg cagcggagaa tttgtcgcgt gcccgctcgc ggagttcggc
     3721 ctgctcaaca accagatgcg cccgagcagc cgacaagtcc ttccgcagcc g
//
LOCUS       DASUGB010000973         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_664495, whole genome shotgun sequence.
ACCESSION   DASUGB010000973 DASUGB010000000
VERSION     DASUGB010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664495"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFV87_18450"
     CDS             <1..574
                     /locus_tag="VFV87_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105808.1"
                     /translation="KKVLDVPLQDGVSIDGLDLSDDGAKLVVIQTTRNPLAALPRGLI
                     SIYDVSTGEKLIAKPQNSSWLRLPSFSHDGRLIAANVSRPNVGSKSVVILGADDGEEL
                     RECSGHADTVSGIAWNQAGTRLTTVSQDGILKFWDASTGLELMSLELQGQRPVPVEFS
                     PDGLSIATIVDGNTISIWRAPVVNVPFPPN"
     gene            complement(627..2303)
                     /locus_tag="VFV87_18455"
     CDS             complement(627..2303)
                     /locus_tag="VFV87_18455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5105809.1"
                     /translation="MIPYTVSNYVMDRLKEQGVEHVFGVPGDFVLGVFHAVEKSGIEC
                     VCTCNELNAAYAADAYARIRGIGALLTTYVVGELSAINGVAGAYAERVPVVQITGCPA
                     TRHYQNRPLLHHTLGDYNIPVEMYKKITCATALLESAESAPAEIDRILRECIRQSLPV
                     YLGIPADIAMQACPKPTQPLAIPPRKTSDPGQLAEAVREAEAMLAEANKPVIVVDGAI
                     QRFGLTGDLRKLIDASGYPFASMMLGKSVIEESHPQFIGLYEGERCREYVRNRVEQAD
                     AILLAGVMLTDFNSGGFTTHLDEGRSIGLNVDRLRIRHHYYDRVDASEFLRELSARLT
                     KREPATLDLKHASGACRHRATQAYQAEAKKPLKLERFFHRISHFLNDKSTMIVETGSA
                     LFAGAEVLLPAGCRFMSQTFFGSIGYTVGATLGASLAAPERRTCLFVGDGSFQVTAQD
                     LSTMIRYETNPIIFLLNNDGYLVERVISDGPFNDLQRWDYARLPEIFGGSYGVKVRTE
                     EDLETALAEANKRTGEVVFVEVVLGKWDSPESMTKAGATMARNNHLTAAV"
     gene            2631..>3610
                     /locus_tag="VFV87_18460"
     CDS             2631..>3610
                     /locus_tag="VFV87_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX5105810.1"
                     /translation="MPTIQAFRGLRYNLGHVGSLSDVITPPYDVISPAFQDELYKKHP
                     ANFIRLELNREEPGDNERSSKYARAAKFLKQWRQEGVLQVDPDPALYVYHQSFQHEGR
                     EVTRRGFMGRVRLERFGEGKIYPHEETHSGPKADRLLLTKACRANLSQIFGLYPDPSN
                     AAQNLLEDAVRGQTPLTATDHLGVVHRMWPVTHVKTIGDVAAILDPLPMFVADGHHRY
                     ETACNYRDWLAEQGPLDPEHPANFVLTQCVSMNDPGLLVLPTHRIFRGVSPMTVHELT
                     TKLGEHFTVRSAGKGPPQADQIWTEIELAGEQGQLAFYTAQDDAWTLARIT"
BASE COUNT          684 a         1163 c         1083 g          680 t
ORIGIN      
        1 caaaaaagtc ctggatgttc ctcttcaaga tggagtcagc atcgacggcc tggatctgag
       61 cgacgatggg gcaaaactcg tcgtcatcca aacgaccaga aacccgttgg cggcactccc
      121 tcgcggcttg atttcgatct acgacgtctc gaccggcgag aagctgatag caaagcccca
      181 gaattcgtct tggctgcgcc tccccagctt tagtcatgac ggtcggttga ttgcggccaa
      241 cgtaagccga ccgaacgtgg gcagcaaaag cgtcgtcatc ctcggcgcgg acgacggaga
      301 ggagctgcgc gaatgcagcg gacatgcgga tacggtttca gggattgcct ggaatcaggc
      361 cggaacgcgg cttacaacgg tcagccagga cggcatcctc aaattctggg acgcgagcac
      421 gggtctggag ctgatgtcgc ttgaacttca gggccagcgt ccggtcccgg tcgagttcag
      481 tcctgacggc ctgtcgatcg ccacgattgt ggatggcaac acgatctcca tttggcgcgc
      541 cccggtggtg aacgtgccgt ttcctcccaa ctgagcagtc attggtttgg ccccgcgcgc
      601 aatacgccgc cgcggattgg ctgacgctag accgcagccg tcaggtgatt gtttcgggcc
      661 atcgtggccc cggctttggt catcgattca ggactgtccc atttgcccaa gacgacttcg
      721 acgaacacga cctcgccggt tcgcttgttg gcttcggcaa gggccgtttc gaggtcttcc
      781 tcggtgcgga ccttgacgcc gtaactgccg ccgaagattt ccggcagcct ggcatagtcc
      841 caccgctgca ggtcgttgaa tgggccgtcg ctgatgaccc gctcgacgag atagccgtca
      901 ttgttcaaga ggaagatgat cgggttcgtc tcgtagcgga tcatcgtcga gaggtcctgg
      961 gccgtgacct ggaacgaccc gtcgcccaca aacaggcacg tccgccgctc tggcgcggcc
     1021 agcgaagcac ccagcgtcgc cccaaccgtg tagccgatcg agccgaagaa cgtctgcgac
     1081 atgaaccggc agcccgccgg cagcagcact tcggctccgg cgaacagggc cgagccggtc
     1141 tcgacgatca tcgtcgattt gtcgttgagg aaatggctga tccgatggaa gaaacgctcc
     1201 aatttcagcg gcttcttggc ctcggcttgg taagcctgcg tggcccggtg gcggcaggcg
     1261 ccgctggcgt gcttgaggtc gagcgtcgct ggttctcgct tcgtcaaacg cgccgacagc
     1321 tcgcggagaa actcgctcgc gtccacgcgg tcgtaatagt gatggcgaat ccgcagccga
     1381 tcgacgttca gcccgatcga ccgcccctcg tcgagatgcg tcgtgaagcc gcccgagttg
     1441 aagtcggtca gcatcacgcc cgcgagcaaa atcgcatccg cctgttcgac gcgattgcga
     1501 acatactcgc ggcaccgctc gccttcatac aggccaatga actgcgggtg cgattcctcg
     1561 atcacgctct tgcccagcat catgctggca aacggatagc cgctggcgtc gatcagcttg
     1621 cgaaggtcgc ccgtgagccc gaaccgctgg atcgcgccat cgaccacaat caccggcttg
     1681 tttgcttcgg ccaacatggc ttccgcctcg cgcactgcct cggcaagttg gcccgggtcg
     1741 cttgtcttcc gtggcggaat agccaaaggc tgcgttggct tcgggcacgc ctgcatcgcg
     1801 atgtcagccg ggatgcccag ataaaccggc aggctctggc gaatgcactc gcggaggatg
     1861 cggtcgatct cagccggcgc ggactcggcc gattcgagaa gcgccgtcgc gcaggtgatc
     1921 tttttgtaca tctcgaccgg gatgttatag tctccaagcg tgtggtgcag gagtggccga
     1981 ttctggtagt gccgagttgc cgggcagccg gtgatttgca cgacgggcac ccgctcggca
     2041 tacgcccccg ccacgccgtt gatcgcgctc agctcgccga cgacgtaggt cgtcagcagc
     2101 gcccctatcc cgcgaatccg cgcataagca tccgccgcat acgctgcatt cagctcgttg
     2161 caggtgcaga cgcactcgat gccgctcttc tcaaccgcgt ggaaaacgcc caggacgaaa
     2221 tcgcccggca ctccgaaaac gtgctccacg ccctgctcct tcaggcggtc catgacgtag
     2281 ttgctgaccg tgtacggaat catcggtggg cctttgcaaa agagcgggcg gcaggcaggc
     2341 gagtcgcccc aggtgggatg gcctcgggac agaatgaggc actttctcat ttaccacacg
     2401 cttcacagct gcgccgtcaa agtttcgcac gcacttcgca actcgttgcc aggccagagg
     2461 ttattacccg ctgcctgtgg cctgctttgg cggtggcagg atagttagaa ttgcaccgat
     2521 tcgcgtaact cattccttag ccacggatga aacactaatg gaacacggat ctcagcacct
     2581 ctgaatccca tccgtgttga ttcgtgttcc ctccgtggct cccaagaaaa atgcccacca
     2641 tccaagcctt ccgcggactt cgctacaacc tcggccacgt cggctcgctc agcgacgtga
     2701 tcacgccgcc gtacgacgtg atctcgccgg ccttccagga tgaactgtac aaaaagcatc
     2761 ctgcgaactt catccgcctg gagctcaacc gcgaagagcc aggcgacaac gagcgctcaa
     2821 gcaagtacgc ccgcgccgcc aagtttttga agcagtggcg gcaggagggc gtgctgcagg
     2881 tcgatcccga tccggcgctt tacgtctatc accagtcgtt ccagcacgag gggcgcgaag
     2941 tcacccgccg tgggttcatg ggacgcgtgc gactggagcg gttcggcgag ggcaagatct
     3001 atccgcacga ggaaacgcat tccggcccca aagccgatcg gctgctgctg acgaaggcct
     3061 gccgggcgaa tctgagccag atttttggac tgtatcccga cccgagcaat gccgctcaga
     3121 acctgctgga ggatgcggtt cgcgggcaga cgccactgac ggcgaccgac catttgggcg
     3181 tggtccaccg catgtggccg gtgacccatg tgaagacgat cggcgatgtc gcggcgattc
     3241 tcgatccgct gccgatgttc gtggccgacg gccatcaccg ctacgagacg gcttgcaact
     3301 atcgcgactg gctcgccgag caaggcccgc tcgatcccga gcatccggcc aacttcgtcc
     3361 tcacgcagtg cgtgagcatg aacgatcccg gcctgctcgt cctgccgacg caccgcatct
     3421 tccgcggcgt ttcgcccatg accgttcacg aattgacgac gaagctcggc gagcacttca
     3481 ccgtccgctc cgccggcaaa ggcccgccgc aagccgatca gatctggacc gagatcgagc
     3541 tggccggcga gcagggccag ctcgccttct acaccgccca ggacgacgcc tggacgctgg
     3601 cccgcatcac
//
LOCUS       DASUGB010000974         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_249451, whole genome shotgun sequence.
ACCESSION   DASUGB010000974 DASUGB010000000
VERSION     DASUGB010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249451"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFV87_18465"
     CDS             complement(<1..1062)
                     /locus_tag="VFV87_18465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5105811.1"
                     /translation="MSLTVVLTILAMLLANGVFAAYELALASVRLDRLRVLAEQKRRG
                     AATALRMKNRMEGSLAVVQLGITLVGAIAAAAGGASIDDSLAPELDKLPGISAHLANV
                     LALILFVIPLSAVTIVVGELIPKVLAIKNAEAVCLWLSPFMRAFALFVYPAVLLFEWL
                     TTTFVRLIERLLPIRRTDDHQTHLHELRAQVNLLRASQVIGMQEERIILQASRLSSMR
                     VQEIMMPEDDIVMVVADAPLSENLVVAHMDLHTRFPVTAKKGDPQAIIGYVTFKDMVL
                     LAKTHPGNPVIREIVRQIISLPQELTLSEALRRMTLEHHHLALVRDKAGAIVGMITQE
                     DIFEELVGDIQDEFDRLPRH"
     gene            complement(1183..2664)
                     /locus_tag="VFV87_18470"
     CDS             complement(1183..2664)
                     /locus_tag="VFV87_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5105812.1"
                     /translation="MRIGILALLQESNTFISQPTRFEQFEQDLLACGEEVRERLAGTH
                     HEVAGMFHALEEEGALAVPIFAARAYPFGVIEGDAIERLLKMMFGELEKAGKLDGVLV
                     APHGATVSRSVPDVDGHWLTEVRRRVGPDVPIVGTLDPHANLSLAMVAATDTLIAYRT
                     NPHLDQRARGIDAGRHICRTIRGDVRPTMAAAYPPLAINIEKQHTAEEPCHTLYKLAD
                     QQLLDERVRFNSIILGFPYADVEEMGSATIVVTDNDAELAQQLANDLAQHIWTHREDF
                     AGEFITIDEALDKAAVMSGPVCLLDMGDNVGGGSPGDGTLLAIAMHERKLPRCFVCIC
                     DSDAVAQAEAMGEWGTGAFRVGGKADRLHGPPLECQATVVGLFDGRFEEPHPRHGGFS
                     HFDQGRTAVIRTDAGLTIMLTSRRLPPFSLRQLTSCGVEPREQHVLVAKGVNAPVGAY
                     KEVAKHFIRVNTPGVTTADMQQLTYQHRRRPMFPFERDFGWKP"
     gene            3082..>3797
                     /locus_tag="VFV87_18475"
     CDS             3082..>3797
                     /locus_tag="VFV87_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEX5105813.1"
                     /translation="MASDVRLKEQLPRLTDRIVAGYKQAGSIHYLGHCPLPNYEEIVC
                     CLEDLKEILYPGYRRREGLHIGNVTYHVGDLIDGLHDKLTQQIARALQHEDRVVTKTS
                     TCENQQDYEAKGQAMAIAFLEKIPALREILATDAKAAFDGDPACKSIDEVVFCYPGFE
                     AITVHRIAHELRRLGVPFIPRMMSEWAHKETGIDIHPGATIGHHFFIDHGTGVVIGET
                     CEIGNHVKIYQGVTLGALSFA"
BASE COUNT          704 a         1237 c         1192 g          664 t
ORIGIN      
        1 gtgccgcggc aggcggtcga attcgtcctg gatgtcgccg accagctcct caaagatgtc
       61 ttcctgcgtg atcatgccga cgatggcccc cgccttgtcg cgaaccagcg ccaggtgatg
      121 gtgctcgagc gtcattcgcc gcagcgcctc gctgagcgtc agctcctgcg gcaggctgat
      181 gatctgccgc acgatctccc ggatgaccgg attgcccggg tgcgtcttgg ccaggaggac
      241 catgtccttg aacgtgacgt agccgatgat cgcctgcgga tcgccctttt tggcggtgac
      301 gggaaaccgc gtgtgcaggt ccatgtgggc gacgaccagg ttctcgctga gcggggcatc
      361 ggccaccacc atcacgatgt cgtcctccgg catcatgatc tcctgcaccc gcatcgacga
      421 gagccggctg gcctgcagga tgatccgctc ttcctgcatg ccgatcacct ggctggcgcg
      481 gagcaggttg acctgggccc gcagctcgtg gaggtgggtc tggtggtcgt cggttctgcg
      541 aatggggagc agccgttcaa tcagccggac gaaggtcgtc gtcagccatt caaacagcag
      601 caccgcggga taaacgaaga gggcgaaggc ccgcatgaac gggctgagcc agaggcagac
      661 ggcttcggcg tttttgattg ccaggacttt gggaatcagc tcgccgacga cgatggtgac
      721 ggccgaaagc gggatgacga acaggatgag cgcgaggacg ttcgccagat gagctgagat
      781 gccgggaagc ttgtcgagct ccggcgcgag tgaatcatca atgctggcgc cccccgcggc
      841 agcggcaatg gccccgacga gcgtgatgcc gagctgcacc accgccagcg agccttccat
      901 gcggtttttc atccgcaggg cggtggcggc cccgcggcgt ttttgctcgg ccaaaacccg
      961 caagcggtcg agccgcaccg aagccagcgc cagctcgtag gcggcgaaca cgccgttggc
     1021 caggagcatg gcgaggatgg tcaggacgac ggtcaggctc atcgcaattc caaggcagac
     1081 aagacgttac gcgaatcggc acccaccccg cgccggcatt gcccgccagt tagagaatac
     1141 cggcggcaga atcgtccgcc aagtcgagga aggggccgca aatcacggct tccagccgaa
     1201 gtcccgctcg aagggaaaca tgggccggcg gcgatgctgg taggtgagct gctgcatgtc
     1261 ggcggtggtg acgccgggcg tgttgacgcg gatgaagtgc ttggcgacct ctttgtaagc
     1321 cccgaccggc gcattcaccc ctttggccac cagcacgtgc tgctcgcgcg gctccactcc
     1381 gcagctcgtg agctgccgca ggctgaaagg aggcaggcgg cggctggtga gcatgatcgt
     1441 caggccggca tccgtccgga tcaccgcggt gcggccctgg tcgaaatgcg aaaagccgcc
     1501 gtggcgcggg tgcggctcct caaatctgcc gtcaaagagg ccgacaaccg tcgcctgaca
     1561 ctccagcggc ggcccgtgca gtcgatcggc cttgccgccg actcggaacg cgccggtccc
     1621 ccattcgccc atcgcctcgg cctgggcgac cgcgtcggaa tcgcaaatgc agacgaaaca
     1681 ccgcggcagc ttgcgctcgt gcatcgcaat ggccagcagc gtgccgtcgc ccggcgagcc
     1741 gccgccgacg ttgtcgccca tatcgagcag gcagaccggc ccgctcatca ccgccgcctt
     1801 atcgagtgcc tcgtcgattg tgatgaactc gccggcaaag tcctcgcggt gcgtccagat
     1861 gtgctgggcc aggtcgttgg ccaactgctg ggccagttcc gcgtcattat cggtcacgac
     1921 gatcgttgcg ctgcccatct cctctacatc ggcataggga aaccccagaa tgatactgtt
     1981 gaagcggacc cgctcgtcta aaagctgctg atcggccagc ttgtagagag tgtgacaagg
     2041 ttcttccgcc gtatgctgct tctcgatgtt gattgccagc ggcggatagg ccgccgccat
     2101 cgtcggacgt acgtcgccgc gaatggtgcg gcagatgtgc cggccggcat cgatcccgcg
     2161 ggcccgctgg tcgagatgtg gattggtgcg gtaggcaatc agggtgtccg ttgccgcgac
     2221 catcgccaat gagaggttcg cgtgcggatc gagcgtgccc acgatcggca cgtcgggccc
     2281 gactcgccgc cggacctcgg tgagccagtg gccgtcgaca tccggaacgg accggctgac
     2341 cgtcgcccca tgcggcgcaa cgagcacgcc gtcgagcttt ccggccttct ccagctcgcc
     2401 aaacatcatc ttcaggagcc gctcgatggc gtccccctcg atcacgccaa acggatacgc
     2461 ccgggcggcg aagatcggca ccgccagagc gccctcttcc tccagggcgt gaaacatgcc
     2521 ggccacttcg tgatgcgtcc ccgccagccg ttcgcggacc tcttcgccgc acgccagcag
     2581 atcctgctcg aactgctcaa accgcgtcgg ctgcgagatg aacgtgttcg actcctgcag
     2641 cagggcgaga atgccaatac gcatgacggc gagcggcggc agctagggaa cgatgcgaag
     2701 atgcaggttg ccattgtaag gcattcgccc caggtcgggg ttatgttgcg aagattggaa
     2761 ttcgccgcga ggccaagcgc ccgcccccgc gaattcccct caacttccgc tgctgcggct
     2821 tccgaagatt ctggcagacg gcttgaatcg agggcctagc gcgcctagga ttcggctaga
     2881 ttttgaagtt gacggcacac gcagtgcgcc acttacttat gcccgcccgc tgcttccatt
     2941 tcggctcctc cttatgccga tgtaacggtt gacgcgattc tgcgcgtcaa tgtcttactg
     3001 gctggcagcg tcattccccc gctagcacgc acccgccggc agttctgcgc gccggcacct
     3061 tcccaaaccg gagtcgagac catggcctcc gacgttcgcc tgaaagaaca gctcccgcgg
     3121 ctgaccgacc ggatcgtcgc cggctacaaa caggccggtt ccatccacta cctcggccac
     3181 tgcccgctgc ccaactacga ggaaatcgtg tgctgcctgg aggatctgaa ggagatcctc
     3241 tatcccggat atcgccgccg cgaagggctg cacatcggaa acgtgaccta ccacgttggc
     3301 gacctgatcg acggcttgca cgataagctc acgcagcaga tcgcccgcgc cttgcagcac
     3361 gaggaccgcg tcgtcacgaa gaccagcacg tgtgaaaacc agcaggacta cgaggccaag
     3421 gggcaggcga tggcgatcgc cttcctggaa aaaatccccg cgcttcgcga aatcctggcg
     3481 accgatgcca aagccgcctt cgacggcgac ccggcctgca agagcatcga cgaagttgtt
     3541 ttttgctatc cgggctttga ggcgattacc gtccatcgca tcgcccatga actccgtcgg
     3601 ctgggagtgc cgttcattcc ccgcatgatg agcgaatggg cccacaaaga aacgggcatc
     3661 gatatccacc ccggagccac gatcggccac cacttcttca tcgatcacgg cacgggagtc
     3721 gtgatcggcg agacatgcga gatcggcaac cacgtgaaga tctatcaggg cgtcacactc
     3781 ggcgcactga gctttgc
//
LOCUS       DASUGB010000975         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_249503, whole genome shotgun sequence.
ACCESSION   DASUGB010000975 DASUGB010000000
VERSION     DASUGB010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249503"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..>2957)
                     /locus_tag="VFV87_18480"
     CDS             complement(228..>2957)
                     /locus_tag="VFV87_18480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5105814.1"
                     /translation="KVFCDNVQRLMSVEVGLGGVWLICLPHLLFIPDRNGDDVPDGSP
                     EVVLDGFGISPENYHNCANGLRWGPDGWLYGRCGASSPGRIGPLGARDEARVPIFGGL
                     WRYHPTRKVFETLCHGTTNPWGHDWDAHGELFFTNTVTGHLFHMLPGAHYTRSATIAV
                     NRRVYEPIDTHADHLHWDTGSGLRDEHAHESLGGGHAHCGAMIYLADQWPEEYRGRLM
                     TLNFHGRRINVERLEREGCGYAARHEPDIAFAEDQWFRGIDLSYGPDGAVYLLDWSDT
                     GEYHEHEGIHRLSGRVFRIAYGDAKPPATLDLTKLSAEELVGLHSHSNEWYVRQARRE
                     LANRPAAGGDSAAAIGGLRPLLDKQENDARLRLQALWTLYALGAVEPVALDKLLDDPD
                     EHVRVWALRLLTDFWPLDSVHGLVRSTPQSIDPRLAERLPEIAKNDSSGLMRLTLAST
                     LQRLPLDMRPPLAAALAAHEENLSDHNLPKLIWYGLAPLAERDPAALVPIAADGKLPL
                     TRQWIARALAEDIAKSGEALDALLAATSSKEEAVRGDVIRGLIAGLAGRHKVAPPIHW
                     QNFQASCESAPEEVKEQVRNLSVTFGDGRALDEVRRVALDDQSRIDQRRLALEALIDA
                     RPDDLRDTCQKLLGVRFLNVTALKGLTLFDDVEIGRELAKNYNRFHPSERAAVLETLV
                     ARPSFAAGLLDQIEAGKIPREELTALHARQIRSFGDESLAARLSEVWGELRDSPQDKQ
                     ELIARLKEQLSGDRLASADKRQGRAVFHATCANCHKLYGAGGAIGPDLTGSGRQNLDY
                     VLSNIVDPSAVVNKDFRMSLVRHIDGRVLSGLMVSQDDERVVLQTVKERLTIPREEIE
                     ELSLSTLSAMPDGLLQPLKDEQIRDLVAYLMSPGQVELPPGFQEVSGTTVSGK"
BASE COUNT          497 a          955 c          969 g          536 t
ORIGIN      
        1 ctcgcgaatc tccacatgcg gctgcacggg gacgccggcc gagtggtcga tcagtttcca
       61 ggtcttggtg ggcattttcg gattcctgat tcgttgagta ttccttacgc aacttcgcag
      121 cgaacttgtc gagtggggcc gataggtcgg gcgtcagcag acacgagcca attgtataac
      181 gacttcgtcc gcgcttgtga cagacgggtt ggaaacccgt cctgcgctca tttgcccgaa
      241 accgtagtcc cgctgacttc ctgaaagccc ggcggcagct cgacctgccc cgggctcatc
      301 aaataggcga ccaggtcgcg aatctgctcg tccttcagcg gctgcagcag gccatcgggc
      361 atggcggaca gggtcgacag ggagagctct tcgatttcct cgcgcgggat cgtcagccgc
      421 tctttgaccg tctgcagcac gacgcgctcg tcgtcctggc tgaccatcag gccgctgagg
      481 acgcggccgt cgatgtggcg gacgagcgac atgcgaaagt ccttgtttac cacggcgctg
      541 ggatcgacga tgttgctcag gacgtagtcg agattctgcc gccccgagcc agtgaggtcc
      601 ggaccgatcg cgccgccggc accgtatagc ttgtggcagt tggcacaggt cgcatgaaag
      661 acggcccggc cttgccgttt gtcggcggat gccagccggt cgccgctgag ctgctctttg
      721 aggcgggcga tcagctcttg cttgtcctgg ggcgaatcgc gcagctcgcc ccagacctcc
      781 gagaggcggg ccgcgagcga ctcgtcgccg aagctgcgaa tctgccgggc gtgcagggcg
      841 gtcagctctt cgcgcggaat cttgcccgcc tcaatctggt cgagcaggcc ggctgcgaac
      901 gacggccggg cgacaagcgt ttccagcacg gcggcccgct ccgagggatg gaaacggttg
      961 tagttttttg ccagctcgcg gccgatttcg acgtcgtcga agagcgtcag ccccttaaga
     1021 gccgtgacgt tcaagaagcg gacgccgagc agcttctggc aggtgtcgcg tagatcatcg
     1081 ggccgggcgt cgatcaaggc ttccaacgcg agccgccgct ggtcgatgcg ggattgatcg
     1141 tccaaagcga cgcggcgaac ctcgtcgagc gcccggccgt cgccaaaagt cacactcaaa
     1201 ttgcggacct gttccttcac ctcctccgga gcggattcgc agcttgcttg aaagttctgc
     1261 cagtgtattg gcggcgcgac tttgtgccgg ccggccaggc cggcgatcag gccgcgaatg
     1321 acgtcgcccc gcacggcttc ttccttggac gaggttgccg ccagtagcgc gtcgagtgcc
     1381 tcgccgctct tggcgatgtc ttctgccagg gcccgcgcga tccactgccg cgtcagcggc
     1441 agcttgccgt cggcagcgat tggtaccaga gcggcaggat cgcgctcggc cagcggcgcg
     1501 aggccatacc aaatcaattt cggcaggttg tggtcgctta gattttcctc atgggcggcg
     1561 agcgccgccg ctagcggcgg ccgcatgtcc aacggcaagc gctggagcgt cgatgccagc
     1621 gtcaggcgca tcagtccgga cgagtcgttc ttggcgatct ccggcagccg ctcggccagc
     1681 cgcggatcga tcgactgcgg ggtcgaacgc acgaggccgt gaaccgaatc cagcggccag
     1741 aagtcggtga gcagccgcaa tgcccagacg cggacgtgct cgtctggatc gtccaggagc
     1801 ttgtcgagcg ccaccggttc gaccgcaccc agtgcataga gcgtccacag cgcttgcagt
     1861 cgaagccgcg cgtcgttctc ctgcttgtcg agaagcggtc gcaggccccc aatggctgct
     1921 gctgaatcac cgccggcagc gggccggtta gccaactccc gccgcgcctg ccgcacgtac
     1981 cactcgttcg aatgcgagtg cagtcccaca agctcctcgg cggagagctt cgtcaagtcc
     2041 aacgtcgccg gtggcttcgc gtcgccatac gcgatgcgaa acacgcggcc ggagagccgg
     2101 tggatacctt cgtgctcgtg gtattcgccc gtgtcgctcc agtcgaggag atagaccgcg
     2161 ccgtccggcc cgtaagacag gtcgatcccg cggaaccact ggtcctccgc gaaggcgata
     2221 tccggctcgt gccgggcggc atagccgcag ccttcccgct ccagccgttc gacattgatc
     2281 cgccggccgt ggaagttcag cgtcatcagc cggccgcgat attcctcggg ccactggtcg
     2341 gccagataga tcatcgcccc gcagtgggcg tggccgcctc ccagcgattc atgggcatgc
     2401 tcgtctcgca ggccgctgcc ggtgtcccag tgcagatggt cggcatgcgt gtcgattggc
     2461 tcatagacgc ggcggttgac ggcgatcgtc gcactgcggg tgtaatgggc cccgggaagc
     2521 atgtggaaga ggtgaccggt gaccgtgttc gtaaagaaca actcgccatg tgcgtcccag
     2581 tcgtgccccc agggattcgt tgtgccgtgg cagagcgttt cgaagacttt gcgcgtcgga
     2641 tgatagcgcc agaggccacc gaagatggga acacgtgctt cgtcccgcgc gccgagcggg
     2701 ccgatccggc cagggctcga agcgccgcag cggccgtaca gccagccatc aggcccccac
     2761 cgcaggccat tcgcgcagtt gtggtagttc tcgggcgaga ttccaaagcc gtcgagaacg
     2821 acctccggcg agccgtcggg cacgtcgtcg ccgttgcggt cgggaatgaa cagcaagtgc
     2881 ggcaggcaga tcagccagac gccgccgagt ccgacctcga cactcatcag ccgctgcacg
     2941 ttgtcgcaaa agacctt
//
LOCUS       DASUGB010000976         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1359717, whole genome shotgun sequence.
ACCESSION   DASUGB010000976 DASUGB010000000
VERSION     DASUGB010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359717"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFV87_18485"
     CDS             <1..783
                     /locus_tag="VFV87_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5105815.1"
                     /translation="FKKPLTIEDLIRFGSITKEIAEFMRACIISRLNTVVSGGTGSGK
                     TTLLNTLSSFIQNDQRIVTIEDAAELQLQQDHVVRLETRPPNIEGKGAITATDLVKNA
                     LRMRPERIIIGECRGPETLDMLQAMNTGHDGSLTTLHANTPRDALARLETMVMMAGFE
                     LPIKAMRQQISSAVQVMIQANRLQGGIRRVTSITEVVGMEQDTIVMQDIYKYVQEGID
                     ETGRARGHFEATGIRPTFMNKLESAGVRLPSSAFKQRTMLAD"
     gene            811..1776
                     /locus_tag="VFV87_18490"
     CDS             811..1776
                     /locus_tag="VFV87_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5105816.1"
                     /translation="MLTILIIAASFIGVACLVGGVASLMFRGESATAMEERLDVLTGA
                     AAPSAAKKQAETKLLASPLNDVPSVLEEFVLKFFNLRAFLQQADTHLSPTKFIIISVG
                     LAVGTTFVLPLARIPLALAPVGVLAGIIPFAYLYLKRKRRLGRFAKQLPESLELISRA
                     LRAGHSLASGFKLVADEQDYPINVEFERCYESQNLGVPLEEAIEQMTERVPNLDLRFF
                     ATAVILQRQTGGDLAEILDKIGHLVRERFRIWGQIQALTGEGRLSGVVLLALPPVLFV
                     VMWRLNPDYCMALFTDPMGHQMLAGAIVMQIIGALVIRKIVNIKV"
     gene            1833..>2377
                     /locus_tag="VFV87_18495"
     CDS             1833..>2377
                     /locus_tag="VFV87_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5105817.1"
                     /translation="MADLLTNPWVVQIAVFGGIAALAWIAVEWLARSNSRAEQRLEEF
                     RDPMARKKREEGAGPKKDAAVSRMLKAAAPTLAKPLQAKTEKEQSKLKLKLGYAGFRG
                     EAAPQIFLMMKVIMLCIGLFVGGGVTFLMYGLTQGALIRLVLIAGGSFYFPDLIVMWL
                     GKKRKEAIFLGLPDALDLMVV"
BASE COUNT          451 a          739 c          742 g          445 t
ORIGIN      
        1 ttcaagaagc cgctgacgat tgaagacctg atccgtttcg gatcgattac gaaggaaatc
       61 gccgagttca tgcgcgcctg tatcatctcg cgtctgaaca cggtcgtatc gggcggcacg
      121 ggctccggca aaacgacgct gctcaacacg ctctccagct tcattcagaa cgaccagcgc
      181 atcgtcacca tcgaggacgc ggccgaactg cagctccagc aggaccacgt ggtgcggctg
      241 gaaacccgcc cgccgaacat cgaaggcaaa ggggcgatca ccgccaccga cctggtgaaa
      301 aacgccctgc gtatgcgtcc cgaacggatc atcatcggcg agtgccgcgg acctgaaacg
      361 ctggacatgc tgcaggccat gaacaccggc cacgacggct cgctgaccac cttgcacgcc
      421 aatacgcccc gcgacgccct ggcccgtctg gaaacgatgg tcatgatggc cggcttcgag
      481 ctgccgatca aagccatgcg gcagcagatc agctcggccg ttcaggtcat gatccaggcc
      541 aaccgcttgc agggcggcat tcgccgcgtg acgagcatta cggaagtcgt cggcatggaa
      601 caggacacga tcgtcatgca agacatttac aagtacgtcc aggaaggcat cgatgaaacc
      661 ggacgggctc gcggccactt cgaggccacg ggcattcggc ccacctttat gaacaagctg
      721 gagtcggccg gcgtgcggct gccgtccagt gccttcaaac agcggaccat gttggccgac
      781 tgagtctcgt acccattccc cgcattcgtc atgcttacga ttctgatcat tgccgcgtcg
      841 ttcatcggcg tcgcctgcct ggtgggcggc gtggccagcc tcatgttccg gggcgaatcg
      901 gccaccgcga tggaggaacg cctggatgtg ctgaccggcg ccgccgcgcc gtccgccgcc
      961 aagaagcagg cggagaccaa gctgctggcc agcccgctca acgacgtgcc aagcgtgctg
     1021 gaagaattcg tcctcaagtt cttcaacttg cgggccttcc tgcagcaagc cgacacgcac
     1081 ctttctccga caaaattcat catcatctcc gtgggcctgg ccgtgggcac gacgttcgtc
     1141 ctgccgctgg cccgaattcc cctggcgctg gcgccggtcg gcgtgctcgc ggggatcatc
     1201 ccgttcgcct acctgtactt gaaacgcaaa cggcggctgg ggcggtttgc caagcaactg
     1261 ccggaatcgc tggagctcat ttcccgggct ctgcgggccg ggcacagcct cgcgtccggc
     1321 ttcaaactcg tggccgatga acaggactac ccgatcaacg tggaattcga gcgctgctac
     1381 gaatcgcaga atctcggcgt gccgctggaa gaagcgatcg agcagatgac cgaacgggtg
     1441 ccgaacctcg acctgcggtt ctttgccacg gcggtcatcc tgcagcggca gacgggcggc
     1501 gatctggcgg aaattctcga caagatcggc cacctcgtcc gcgagcggtt ccggatctgg
     1561 ggccagattc aggccctcac cggcgaaggc cgcttgtcgg gtgtggtgct tttggctttg
     1621 ccgccagtgc tgttcgtcgt gatgtggcgg ctgaatccgg actattgcat ggccttgttc
     1681 accgatccga tggggcacca gatgctggcc ggcgccatcg tcatgcagat cattggggcg
     1741 ctggtgattc ggaagatcgt caatatcaag gtttagtggt gagtgcgtga gtcgtgagtg
     1801 gtgagtagta ctcgcagatt aaggttccgt tcatggctga ccttttgaca aatccctggg
     1861 tcgtgcaaat cgcggtgttt ggcggcatcg ccgccctggc gtggattgcc gtcgagtggc
     1921 tcgcgcgctc caattcgcgg gccgagcagc ggctcgagga gttccgggac ccgatggccc
     1981 gcaaaaagcg cgaggaaggc gccggtccca agaaagacgc agccgtctcc cgcatgctca
     2041 aggcggccgc gcccacgctg gccaagccgc tccaggccaa gaccgaaaag gagcagagca
     2101 agctcaagct gaagctgggc tatgccggct tccggggcga agctgcgccg cagatcttcc
     2161 tgatgatgaa ggtcatcatg ctctgcatcg gcctgttcgt cggcggcggc gtcacgttcc
     2221 tgatgtacgg cctgacacaa ggggcgctga tccgcctggt gctgatcgcc ggcggcagct
     2281 tttatttccc cgacctgatc gtcatgtggc tcggcaaaaa gcggaaagaa gcgatctttt
     2341 tgggcctgcc ggacgcgcta gacctgatgg tcgtctg
//
LOCUS       DASUGB010000977         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1359790, whole genome shotgun sequence.
ACCESSION   DASUGB010000977 DASUGB010000000
VERSION     DASUGB010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359790"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFV87_18500"
     CDS             <1..638
                     /locus_tag="VFV87_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105818.1"
                     /translation="NSIAPNIWRVRADKLERLVSTAIAKHLERVQGGCPISGATLQDA
                     SSTELLGLVKGVHLRQGLVEIILESSSLHKYLPQVEATPSKSITHISAPFQIRRANQE
                     ACLLLGNHPGTVDRRLLEFVSRAAAWWQTILEGENIIEIARRYQVSARQIRMHLPSAF
                     LAPDIIEAIVDGRQPPSLTVGALRSGKVPMDWAEQRKLFSIGSSPRRSLSI"
     gene            1132..1401
                     /locus_tag="VFV87_18505"
     CDS             1132..1401
                     /locus_tag="VFV87_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105819.1"
                     /translation="MSTPQRMRKRAASPPASGGEHDARPRRPPLFKRLPDMSDAQLTS
                     LQKAATRISLDVEHPKRANATIALPLIDAEIGRRATALAEGGPVR"
     gene            1443..2165
                     /locus_tag="VFV87_18510"
     CDS             1443..2165
                     /locus_tag="VFV87_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105820.1"
                     /translation="MLLAIFFMAPARAQTMRVDDIPENAITTTYGRGWECAHGFIERD
                     AACRKVELPTNAYLSAIGSGWSCERGYRQAEEQCVRVVIPENANPTNDIYTKGWACSR
                     GFIERDGRCDGIVVPANAFLTGQAFGPGWECERGYTVVSDQCRAVLLPKHGYLDRSGK
                     TWVCDFGYRRVGEACLAVQVPANGYLNASGSEWSCDRGFARQDNACIGFTTPENAHLD
                     TFGERWECNRGFVRKDAACVRR"
BASE COUNT          511 a          741 c          737 g          450 t
ORIGIN      
        1 cgaactcaat cgcgccgaac atttggcgcg ttcgggctga taagttggaa aggttggttt
       61 ccacggcgat cgcaaaacac ctggaaagag tgcagggcgg ttgcccaatc agcggcgcca
      121 cattgcagga tgcctcatcg actgagctcc ttggtctcgt gaagggggtc cacctccgtc
      181 aaggattggt cgagatcatt ttggagagct ccagccttca caagtacttg cctcaggtag
      241 aggcaacacc atcgaagtcg atcactcaca tctcggcgcc gttccaaatt cgtcgcgcaa
      301 atcaggaagc ctgcctgctg ctcgggaatc accccggtac cgtcgaccgg cggctcctcg
      361 aatttgtttc gcgagccgcc gcttggtggc agaccattct agaaggtgag aacatcatcg
      421 aaatcgcgag gcgttaccaa gtgtcggcac gccagatcag gatgcatcta ccttccgctt
      481 tcttggcgcc ggacatcatc gaagcgatcg tcgatggtcg acagccacca tcgctgactg
      541 ttggagcatt gcggagcgga aaagtgccga tggattgggc agaacaacga aagcttttca
      601 gtattgggtc gtcgccccga cggtcattga gcatctgaag cgcccacaat tgcaagttat
      661 ccgcacgtcg aacaaaaaga gcgccgttga tcggtcagac tgcgccgcac aggtgcatcg
      721 ggcggcatat aggggtaaga aacctgatgc caaacgaaag cgaactgcaa agacgggcgc
      781 gaagcttgcg cggagaaatc gcaggtctag agcgtgcgat cgccgtgatg tccgcataca
      841 atgaggaaga ccggcgcgag cagctggaaa cgctacgtac agtgcgcggt cttcgtgagc
      901 gcgagttgca cgatgtcgaa ggccaattgt cggaacagca atagggcggt cgtcgtgcgt
      961 tagggcggtc tggcgcggaa aaaacaaaca cctcgcgcgc caggtgcaaa tcgggtatac
     1021 gaactccagc ttttcagaaa aatttggtcc gttctggaat tcgtcgtgcc ttctggcccg
     1081 cacccggcga agattcgccg gggcgtcccg gccatgccga aaggaagtcc catgagcacc
     1141 ccccaacgga tgcgcaaacg cgctgccagc cctcctgcgt ccggcggtga acacgacgca
     1201 cggccccgtc ggccgccgct gttcaaacgt ctgcctgaca tgagcgatgc gcagctgacg
     1261 tcgcttcaga aagccgccac gcgaatctct ctggacgtcg agcacccgaa gcgcgccaat
     1321 gcgaccatcg ccctgccgct gatcgacgca gagatcgggc gccgcgccac cgctctcgca
     1381 gagggtgggc cagtgcgatg aacaactgct tcatgcgcac atgccttgga agtctcgccg
     1441 ccatgcttct cgccatcttt tttatggcgc cggcgcgggc ccagacgatg agggttgacg
     1501 atattcctga aaacgcaatc accaccacct atggccgtgg atgggagtgc gcccacggct
     1561 tcattgaacg ggacgctgct tgccggaagg tcgagctgcc gaccaatgct tatctcagtg
     1621 cgattggcag cggctggagc tgcgaacgtg gataccgcca ggcagaagag cagtgtgtcc
     1681 gcgttgtgat tcccgaaaac gccaatccca cgaacgacat ctacaccaag ggctgggcct
     1741 gctcccgcgg tttcatcgag cgcgacggcc gctgcgatgg aattgtcgtg ccggccaacg
     1801 ccttcctcac cggccaagca ttcggtcccg gctgggagtg cgaacgcggc tatacagtcg
     1861 tcagcgacca gtgccgggct gtccttctcc ccaagcacgg ctatctcgac cgctcgggca
     1921 agacgtgggt ctgtgatttc ggttaccggc gcgtcggcga ggcttgcctc gctgtccagg
     1981 ttcctgccaa tggctatctc aatgcctcgg gaagcgaatg gtcctgcgac cgtggattcg
     2041 cccgacagga caacgcctgc atcggcttca cgacaccgga gaacgctcat ctcgacacgt
     2101 tcggcgagcg ctgggaatgc aatcgcggat tcgtgcgcaa ggatgccgcc tgtgtccggc
     2161 gctgaaagcc tcacctgaaa gcattggccg gctccggcca gcacggccgg acgcccagca
     2221 ttgcccaatg cagccagcgc gcgatcgtcg atcccacgca tgcgccagaa tgaggagcca
     2281 cgacaagaga cctcgggcca agcgcctggt tgctttccgg aggtcgtgcg ctatttcggc
     2341 ggatatcgct ggcattgaaa gatggggcgc ctgcggctga gagacgttgt gctcccgctc
     2401 gacagccttc tgagtgcgaa tgcccccgga aaccacacg
//
LOCUS       DASUGB010000978         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_249709, whole genome shotgun sequence.
ACCESSION   DASUGB010000978 DASUGB010000000
VERSION     DASUGB010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249709"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VFV87_18515"
     CDS             complement(<1..1386)
                     /locus_tag="VFV87_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105821.1"
                     /translation="MYPCHRISPLPLDRRSFLRQAGGGFGAVALAALLADEELGGGRM
                     LAADETRESDPLAPRKPHFAAKAKSVIYLYMDGGPSQVDTFDPKPRLAADNGKPFAMK
                     IEPTQFNNNGATLGSPWKFQQYCQSGIPVSELFPGVAQHVDKLAVIRSMTSQFPEHTS
                     ANYFLHTGTGIQGRPSMGAWFGYGLGNDCRDLPGFVVINGGLIPPGGLDNFNSGFLPA
                     SYQGSIFTAANPPVANINRNEATDQLQKNKLALLKKLDEERLAVTGKNSAVESAIANY
                     ELAYRMQTVVPGLTDLKDESAATQAMYGLDAKFPHTASFARQCLLARRLVERGVRFIE
                     LTCPNCGHDRWDQHSGLKKGHEDNALAVDQPIAALLTDLCQRGLLDSTLVVFSGEFGR
                     TPFAQGSDGRDHNPFGFSLWLAGGGIKGGTIYGQTDEFGYKAVEDKSEIHDLHATMLH
                     LLGIDHTRLTFR"
     gene            complement(1404..>2588)
                     /locus_tag="VFV87_18520"
     CDS             complement(1404..>2588)
                     /locus_tag="VFV87_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEX5105822.1"
                     /translation="ADYLQEFEAKATKLGAKVHWAKDAAEHNEIVLAILQGHSVKRLV
                     KSKSMLTEECHLNPFLERHGIEVVDTDLGERIVQLAGKPPSHIVLPAIHLKKEDVGQL
                     FHEHLGTKEGATDPQYLAEVARQHLRDKFMEADAGLTGVNFAVAETGGFVICTNEGNA
                     DLGVSLPKLHIACMGIEKIIPRAKDLGVFLRLLARSATGQPITTYSSHFHGPRDGAEL
                     HIVLVDNGRSNILGSDDFRRSLNCIRCGACMNTCPVYRRSGGYSYESTVPGPIGSILA
                     PARDAKAYATLPSACSLCGSCTDVCPVKIDIHHQLLTWRREIAAHGHLALSKRLAMQF
                     ASFVFRRTWLYSLAGKLARFLGRWTPRFLIYNPLNRWGKQRDLPPIPKRSFREEFKRR
                     RK"
BASE COUNT          448 a          762 c          861 g          517 t
ORIGIN      
        1 gcggaaggtg aggcgggtgt ggtcgatgcc gagcaagtga agcatcgtgg catgcaggtc
       61 gtggatttcg gacttgtctt cgaccgcctt gtagccgaat tcgtcggtct ggccgtagat
      121 ggttcctccc ttgatgccgc cgccggcgag ccagaggctg aagccgaagg gattgtggtc
      181 gcggccgtcg gagccttggg cgaatggagt gcggccgaat tcgccggaga agacaacaag
      241 cgtgctatcg agcaggccgc gctggcagag gtcggtcaag agggcggcga tgggctggtc
      301 cacggcgagg gcgttgtcct cgtgcccttt cttgaggccg ctgtgctggt cccagcgatc
      361 gtggccgcaa ttggggcagg tgagctcgat gaagcgcacg ccccgttcta ctaatcgccg
      421 cgccagcagg cattgccggg cgaaggaggc cgtgtgggga aacttggcgt cgaggccgta
      481 catcgcttgc gtggcggcgg attcgtcctt gagatcagta aggcccggaa cgacggtttg
      541 catgcgatag gccagctcgt agttggcaat tgccgattcc accgcgctat tttttcccgt
      601 caccgccagg cgttcttcat cgagcttctt gaggagggcg agcttgttct tctggagctg
      661 gtccgtcgcc tcgttgcggt taatgttggc gacaggcgga ttggccgcgg taaatatcga
      721 gccttgatag cttgccggca gaaagccgct gttgaaattg tcgagaccgc cgggggggat
      781 cagcccgcca ttaatcacga caaaccccgg caggtcacgg cagtcattgc cgagtccata
      841 gccgaaccag gctcccatgc tgggccgccc ttggatgccg gtgccggtat gcaggaagta
      901 gttcgcgctc gtgtgctcgg ggaactggct ggtcatactg cggatgaccg ccaacttatc
      961 gacatgctgg gcgacgccgg gaaacagctc gctgaccgga atgccgctct ggcagtactg
     1021 ttggaacttc cacggagaac cgagcgtggc cccattgttg ttgaactgcg tcggctcgat
     1081 cttcatggcg aacggcttgc cgttatccgc cgccagccgc ggcttgggat cgaaggtatc
     1141 gacctggctg ggcccgccgt ccatgtagag atagatgacg ctcttggcct tggcggcgaa
     1201 atggggctta cgcggagcga gagggtcgct ttctctcgtc tcatccgccg ccagcattcg
     1261 tccgccgcca agttcttcgt ccgccaaaag cgccgccagt gccaccgcgc caaatccgcc
     1321 ccccgcctgc cgcagaaagc tgcggcggtc gagaggaaga ggggaaatac ggtgacacgg
     1381 gtacatacca gcagtatatc cttctacttc ctccgccgct tgaactcttc ccgaaagctt
     1441 cgctttggaa tcggcggcag atcccgctgt ttgccccagc ggttgagcgg attgtagatc
     1501 aggaatcgcg gtgtccagcg gccgaggaag cgagcgagct ttcccgcgag tgagtagagc
     1561 caggtccggc ggaagacgaa actcgcaaat tgcatcgcca accgcttgga gagggcgagg
     1621 tggccgtggg cggcaatttc ccgccgccag gtgagaagct ggtggtggat gtcgatcttc
     1681 acggggcaga cgtccgtgca ggagccgcat aggctgcagg cactgggcag tgtggcgtag
     1741 gcctttgcgt cacgggcggg ggcgagaatg ctgccgatgg ggccggggac agtgctctca
     1801 tagctgtaac ccccgctacg gcggtagacc gggcaggtgt tcatgcaggc cccgcagcgg
     1861 atgcagttca gcgagcggcg gaagtcgtcg ctccccagga tattgctccg gccgttgtcc
     1921 accagcacga tgtggagctc ggccccatcg cggggaccat gaaaatgcga cgagtacgtc
     1981 gtaatcggct ggccggtggc ggagcgggcg agcaggcgca ggaagacgcc gaggtccttg
     2041 gcccgcggaa tgattttctc gattcccatg caggcgatgt gcagcttcgg cagcgagacg
     2101 ccaagatccg cgttcccttc gttcgtgcag atgacaaagc cgccggtttc cgccacggcg
     2161 aagttgacgc cagtcagccc ggcatccgcc tccatgaatt tatcccgcag atgctggcgg
     2221 gccacttcgg ccaggtactg cgggtcggtt gccccttcct tggttcccaa atgctcgtgg
     2281 aagagctggc ccacgtcctc ctttttcaag tgaatcgccg gcagcacgat gtggctcggc
     2341 ggcttgccgg cgagttgcac gatccgttcg ccgaggtcgg tatcgacaac ctcgatcccg
     2401 tgccgctcca gaaacggatt gaggtggcat tcctcggtca gcatcgactt gctcttcacc
     2461 agccgcttga cgctgtgacc ttgcaggatg gcgagcacga tctcattgtg ctcggctgcg
     2521 tccttggccc agtggacctt cgccccgagc ttcgtcgcct tcgcctcgaa ctcttgcagg
     2581 tagtccgc
//
LOCUS       DASUGB010000979         3385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_387567, whole genome shotgun sequence.
ACCESSION   DASUGB010000979 DASUGB010000000
VERSION     DASUGB010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387567"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFV87_18525"
     CDS             complement(<1..858)
                     /locus_tag="VFV87_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105823.1"
                     /translation="MAKERRQPPAQPPAEPAGPVARWIGPLRRLPGWSSQHPLRAGLV
                     AGGLLVAMIGLGAAAALVFRVPDEQDREELLAQALEALDLHDFRQARHLALQMGDAKS
                     LPYGDQAGPLLIHGAVTAHEAEGQTDPTKRRVLYLVAARYLEEARSHGWPQGQEPRGL
                     VLLGRALHHSGRYGESLPILREALAADPASAPQLHWLLTDSHLQSSPPRLDAALTHNR
                     EYLASPELTAHERHRGQLLQGDILLRQGQFEAAYVAAGEVPETASHHPDSVVLQGRVA
                     LAALAKSQPL"
     gene            complement(1006..1491)
                     /locus_tag="VFV87_18530"
     CDS             complement(1006..1491)
                     /locus_tag="VFV87_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HEX5105824.1"
                     /translation="MSTYRVRLEKEHHVFSAAHFITFNGDVCERLHGHNYRVAVEVDG
                     PLDENQYVIDFIALRDELQAITDELDHHVLLPTGHSLLRVTVGDQQVEVDFTPDGRRW
                     VFPRGDCVLLPVANTTAELLANYIGQRLLAAIERRTGQRPARLQVAVDENHGQWGVCE
                     L"
     gene            1602..3176
                     /locus_tag="VFV87_18535"
     CDS             1602..3176
                     /locus_tag="VFV87_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5105825.1"
                     /translation="MRSVSLLPVFFAVCASFCCTSPAPAADTRPNILFIMSDDHAYQA
                     IGAYGSNRNETPNIDRIAKEGMRFDRAFVTNSICGPSRAVILTGKYSHLNGYPDNAGN
                     ARFNGRQQTVAKLLQAAGYNTAMVGKWHLNSDPTGFDYWHILIGQGPYYNPRMKTNGG
                     FVNHDGYTTDIITDVALDWLKNKRDPDKPFFLMYHHKAPHRNWQPAPRHLDKYKDVEV
                     PEPATLFDDYSGRTKPAAENQMSVARHLTPNDLKLVPQGGLKPDQQAAFDKAYGEENE
                     KLKAAKLTPEQQTKWNYQRFVKDYLRCVDAVDENVGRVLDYLDESGLAKNTIVFYTSD
                     QGWYLGEHGWYDKRWMYEESFRTPLLVRWPGKAKPGSVSDKLVMNLDFPETFLDIAGV
                     EIPDDMQGKSIVPILEGQDTPDWRKSVYYHYYEFPQPHYVHPHTGVRTERYKLIHFYT
                     IDAWELFDLEKDPNELKSVYDDPEYAKIVTDLKHELQRLKDKYQDDGTVVKFEDEAPQ
                     PMPKGKAKGKAKAKQA"
BASE COUNT          673 a         1084 c         1061 g          567 t
ORIGIN      
        1 gagcggctgg gatttggcca gcgccgccag cgccactcga ccttgcagaa ccacggaatc
       61 ggggtggtgc gaagccgtct ccgggacttc gccagcggct acgtaggcag cctcgaattg
      121 tccctgtcgc aggagtatgt cgccctgcaa taattggccc cgatggcgct cgtgcgccgt
      181 caattcaggg ctggcgaggt actcgcgatt gtgcgtcagc gccgcatcca gtcgcggcgg
      241 cgagctctgc aaatggctgt cggtcagcag ccagtggagc tgcggcgcgg acgccggatc
      301 cgccgccagg gcctcgcgca ggatgggcag gctctcgcca taacggccgc tgtggtgcag
      361 cgcgcggccc agcaacacca gtccccgcgg ctcctggccc tggggccagc cgtggctgcg
      421 ggcttcttcc aagtaccggg cagcgacgag atacagtacc cggcgcttgg tcgggtcggt
      481 ctgcccttcg gcttcgtgcg cggtgaccgc tccgtgaatc agcaggggcc cagcctgatc
      541 gccgtaagga agggatttcg catcgcccat ctgcagggcc aggtggcggg cctgacggaa
      601 gtcgtgcagg tccagcgctt ccagcgcctg tgccagcagc tcctcgcgat cttgctcatc
      661 aggcacccga aagactaaag ccgcagccgc gcctagtccg atcatggcga cgagcaaacc
      721 gccggccacg aggcccgccc gcagtggatg ctggctggac cagcctggca atcggcgtag
      781 cggaccgatc cagcgcgcaa ccggtccagc cggctctgcc ggcggctgag cagggggttg
      841 tcgccgctcc ttggccattg tctgccttcc tggcaatcct gcgtgcgcgc gaagcctctg
      901 tcgcgcggaa aatgagcggc gggtatgtac tcgaaccgac gggctgctgt caatcggcgt
      961 tcggtgctag ctgccttttg cgatccgatc gcgcccacct gatggctata gctcgcacac
     1021 accccactgc ccatggttct catcgacagc cacctgcagt cgcgccggac gctggccggt
     1081 gcggcgctcg atggccgcca ggagacgttg gccaatgtaa ttggccaaga gctcagcagt
     1141 cgtgttggcc acgggcaaaa ggacgcagtc gccccgcgga aagacccacc ggcgtccatc
     1201 cggcgtaaag tcgacctcga cctgttggtc tcccaccgtg acccgcaaaa gcgaatggcc
     1261 ggtcggcaag agcacgtggt ggtcgagttc gtcggtgatc gcctgcagct cgtcgcgcag
     1321 ggcgatgaag tcgatcacgt actgattctc gtcgagcggg ccgtccacct cgaccgccac
     1381 gcgatagttg tgtccgtgca atcgctcgca cacatcaccg ttaaacgtga tgaaatgcgc
     1441 ggcgctgaag acgtgatgct ccttctcgag tcggacgcgg taggtggaca tgcgtgagcg
     1501 gtgaatagtg gtgggcgagt agtgagtgta ctcagctgtc cggaattagt agtctatacg
     1561 ggtcaatact ccgattcctc gcaacgcgtg agggcctctc catgcgctcc gtttccttgt
     1621 tgcccgtctt cttcgcagtc tgtgcgtcct tctgttgtac cagcccggcc cccgctgccg
     1681 acacccgccc caacatcctc ttcatcatgt ccgacgatca tgcctatcag gcgattggcg
     1741 cctatggcag caatcggaac gagacgccga atatcgaccg cattgcaaaa gaggggatgc
     1801 ggttcgaccg ggccttcgtc accaactcga tctgcggccc cagccgggcc gtgatcctga
     1861 ccggtaagta cagccatctg aacggctatc cagacaacgc cggcaacgcc cgcttcaatg
     1921 gccgccagca gaccgtggcc aagctgctgc aggccgccgg ctacaacacg gcgatggtcg
     1981 gcaaatggca cctcaacagc gatccgacgg gattcgacta ctggcacatc ctgattggcc
     2041 aggggccgta ctataacccg cgaatgaaga ccaacggcgg cttcgtcaac cacgacggct
     2101 acaccaccga catcatcacc gatgtcgcgc tcgactggct gaagaacaag cgtgacccgg
     2161 acaagccgtt cttcctgatg tatcaccaca aggcgccgca tcgcaactgg cagcccgcgc
     2221 cgcggcacct ggacaaatac aaggatgtgg aggtgcccga gccggcgacg ctcttcgacg
     2281 attactccgg ccgcaccaag ccggctgccg agaaccagat gagcgtcgcc aggcacctga
     2341 cgccgaacga cctgaagctc gtcccgcaag gcggcctgaa gcccgaccag caagcggcct
     2401 tcgacaaggc ctacggcgag gaaaacgaaa agctgaaggc cgcgaagctg actcccgagc
     2461 agcagacgaa gtggaactac cagcggttcg tcaaggatta cctccgctgc gtcgatgcgg
     2521 tcgatgagaa cgtcggccgc gtgctcgatt accttgacga atcgggcctg gcgaagaata
     2581 ccatcgtgtt ttacacgtcc gaccagggct ggtacctggg cgagcacggc tggtacgaca
     2641 agcggtggat gtatgaagaa tcgttccgca cgccgctgct cgtgcgctgg cccggcaaag
     2701 cgaaacccgg cagcgtgagc gacaagttgg tgatgaatct cgactttccg gagactttcc
     2761 tcgacatcgc cggcgtcgaa atcccggacg acatgcaggg caagagcatc gtgccgattc
     2821 tagaagggca ggacacgccc gactggcgga agagcgtcta ttaccactac tacgaattcc
     2881 cgcagccgca ctacgtccat ccgcacacgg gcgtgcggac cgagcgctac aagctgatcc
     2941 atttctacac gatcgacgcc tgggagctgt tcgacctgga gaaggatccc aacgagctga
     3001 agagcgtcta tgacgacccg gaatacgcca agatcgtgac cgacctcaag cacgagctgc
     3061 agcggctgaa ggacaagtac caggacgacg gcacggtggt gaaatttgaa gacgaggcgc
     3121 cgcagccgat gcccaagggc aaggccaagg gcaaagccaa ggccaagcag gcctgaagga
     3181 agtccgtcgc cgcctccccg tcgcgagggt tcagcggtta tcctcacgga atggcttctg
     3241 gctcgaagaa actgcgtctg ggcactcgca gcagcccgct ggcccgttgg caggccgaat
     3301 gggtggcgag cgagctgcgc tcacgcggcg tggaaatcga gctggtgccg atcacgacgc
     3361 agggggacgt gaaaagcgga ccgct
//
LOCUS       DASUGB010000980         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1915144, whole genome shotgun sequence.
ACCESSION   DASUGB010000980 DASUGB010000000
VERSION     DASUGB010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915144"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1132
                     /locus_tag="VFV87_18540"
     CDS             155..1132
                     /locus_tag="VFV87_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX5105826.1"
                     /translation="MLRTTRHLLEMIRFSHTLFALPFALLAALMAWTTPGTPQRAFPT
                     DSHSPLTTHSSPFTAHHPAPPFRLQDLTGILLCMVAARSAAMAFNRLADRRIDAENPR
                     TKSRHLPAGTLSVASVVLFTLISAGVFVAGTLLFLPNRLPIILSIPVLLLLLGYSYTK
                     RFTALAHFWLGAALMLAPVCVWIALRGEVLLVNPWDILPAVMLGGAVLAWVSGFDIIY
                     ACQDVEFDRTQRLRSVPASLGVAGALRLAAVCHLVTLGLLAALPLVCPQVPLGWIYGT
                     GVGVIAALLAYEHLLVRPDDLTRVNIAFFHINAIISIGLFVVGAIDLLT"
     gene            1179..2279
                     /locus_tag="VFV87_18545"
     CDS             1179..2279
                     /locus_tag="VFV87_18545"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5105827.1"
                     /translation="MFRSCLLAAGCFALAIGCNSSNQQPAPAAGTSSFSQPDQATQPS
                     ESSVESSGQESATHTSIQGPPTKAEMSLRELAARLVVSDGKGGWRIAEQPATELEKLG
                     PQASADLLPLLKDTSAPVRRGAAFYLLGQFDPAQADQVAAFSALLDDDDQTLRGFGLT
                     AVKQMREKDQIAALPRLTAMLTSMREDKAENRASIARLIGSLKEDASSAVDSLATAAA
                     DDPDAKVRAACLAALVQVAQPALCLLPLANGLSDADPAVRLVAAARLRLMGPVAAPAA
                     KQLAAALGDSDARVGENAAEALIRIGAPAVEPAMAQLASQNIEAKKLALACLAKIGPD
                     AKSALPAIEKCRSDADPVVRQLAEAAIKRITM"
     gene            complement(2269..3147)
                     /locus_tag="VFV87_18550"
     CDS             complement(2269..3147)
                     /locus_tag="VFV87_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105828.1"
                     /translation="MGSTAGAIGRWRTGRIGIGPLALRQALGLTALGLLVFLAVLFCT
                     RRLSGALAVPLGGAGLIAAAVAVGGLAAVGRVLALAARRGSALPKTSSTEYSVLSTQY
                     LAANPGEHPFHASLLRLAAHVVVPSLAATVFLASLTSREAPAWAVAIAWFLLVASEGT
                     SWLVLSRRRSAARARSRWETEQTPVAEPDESIPANIVQQLTRTREGEGEALHALVRAR
                     IPAGDTQAVVHLAFCPPLASPPELTAHAIDADDIAVRITTAETYGLRLEVRSAAKASA
                     DKSVLVEVIGRAGVTS"
     gene            complement(3184..>4165)
                     /gene="metK"
                     /locus_tag="VFV87_18555"
     CDS             complement(3184..>4165)
                     /gene="metK"
                     /locus_tag="VFV87_18555"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5105829.1"
                     /translation="KAQVDYEKVARRVIREVGYTDNDIGFCADTCEVLVKLHSQSPDI
                     AMGVDKDGAGDQGLMFGYACDETPELMPLPIALAHRILNRLTKARQAGEVKWLRPDSK
                     SQVTVEYDGDRAVRIDTVVVSTQHAPDVTQKQIYDFVVGKIIGPELPKELVSGDIKYH
                     INPTGQFIVGGPHGDSGLTGRKIIVDTYGGWGRHGGGAFSGKDPTKVDRSAAYMARHV
                     AKNIVAAELAERCEVQLAYAIGVADPVSVHVDTQGTGRVDDERLCEVVRQVFPLTPRG
                     IINYLDLRRPIYQKTAAGGHFGRSDPDFRWESTDKAAALAEAAGATLAAR"
BASE COUNT          640 a         1492 c         1316 g          717 t
ORIGIN      
        1 cgccgccctc ggagagcttg ccgtcgtcca cggggcgatt gccctggcgg cagacgaaca
       61 aggcgagcgg ggggcgtcag ccggcgagcg gggggcgtca gccccctgag accacgcagg
      121 tgaaggaagc ccccgtattg cggttgaaac aaccatgctc cgcacgaccc gccacctcct
      181 ggagatgatc cgtttcagcc acacgctgtt tgcgctcccc tttgcgctcc tggcggcgct
      241 gatggcctgg acgacgcccg gaacgccaca gagggcgttc cctacagatt cccactcacc
      301 actcaccact cactcctcac cattcaccgc tcaccaccca gctccgccct tccgcctcca
      361 ggatctcacc ggcatcctcc tctgcatggt cgccgcccgc agcgcggcga tggctttcaa
      421 ccggctggcc gaccggcgaa tcgatgccga aaacccgcgc accaaatcgc gccatctgcc
      481 ggcgggaacc ctctcggtcg ccagcgtcgt gctgttcacg ctgatctcgg ccggcgtctt
      541 cgtcgccggc acgctcctgt ttctgcccaa tcgcttgccg atcatcctgt ccattcccgt
      601 gctgctgctg ctgctcggct acagctacac gaagcggttc acggcgctgg ctcatttctg
      661 gctgggcgcg gcgctcatgc tcgctccggt ctgcgtctgg attgcgctgc ggggcgaagt
      721 cctgctggtc aatccgtggg acatcctgcc tgccgtcatg ctgggcggcg cggtgctggc
      781 ctgggtatcg ggtttcgata tcatctacgc ctgccaggac gtcgagttcg accgcacaca
      841 gcgcctgcgc agcgtgccgg cgtcgctggg cgtggccggc gctctccgcc tggcagccgt
      901 ctgccaccta gtcacactcg gcctgctcgc cgcgctgccg ctcgtctgcc cgcaggtgcc
      961 gctgggctgg atctatggga cgggtgtcgg cgtcatcgcg gcgctgttgg cgtacgagca
     1021 cctgctggtg cggcccgacg acctgacgcg cgtcaatatc gctttcttcc atatcaatgc
     1081 gatcatcagc atcggcctgt ttgtcgtcgg cgcaatcgac ctgctgacgt agaatgcagg
     1141 gttgcggggc attcctctcc acgatggtcc catttcgcat gtttcgatct tgccttctgg
     1201 cggccggctg cttcgctctg gcgatcggct gcaattcctc gaaccagcag cccgcccccg
     1261 cagccggcac gtcttcgttt tcccagccgg accaggcgac gcagcccagc gagtcctcgg
     1321 tcgaatcctc cgggcaagag agcgccacgc acacttcgat tcaaggcccg cccaccaaag
     1381 ccgagatgtc gctgcgcgaa ttggcggccc ggttggtcgt cagcgacgga aaaggcggct
     1441 ggcgaatcgc cgaacagccg gcgacggagc tggagaagct cggaccccag gccagcgccg
     1501 acttgttgcc gctcttgaag gacacgtccg ccccggtccg ccgcggcgcg gcgttctacc
     1561 tgctcggcca gttcgacccg gcacaggccg atcaggtggc cgccttctcg gcgctgttgg
     1621 acgacgacga ccagacgctc cgcggcttcg ggctgaccgc cgtcaagcag atgcgcgaaa
     1681 aggaccagat cgctgccctg ccgcggctga cggcgatgct cacttccatg cgggaagaca
     1741 aggcggagaa ccgcgcttcc atcgcccggc tgatcggctc gctcaaagag gacgcgtcgt
     1801 ccgccgtcga ttcgctggca accgcggctg ccgacgatcc cgatgccaag gtccgcgccg
     1861 cgtgcctggc ggcgctggtg caggtcgccc agcccgccct gtgcctgctg ccgctggcca
     1921 acggcctgag cgatgccgac ccggcggtgc gactggtcgc cgccgcccgg ctgcggctga
     1981 tggggccggt cgctgcaccg gccgcgaagc agctcgccgc tgccttgggc gacagcgacg
     2041 cgcgcgtcgg cgaaaacgcc gccgaagcgc tgattcgcat cggcgcaccg gctgtcgaac
     2101 cggcgatggc gcaattggct tcgcaaaaca tcgaggcgaa aaagctcgcc ctggcctgcc
     2161 tcgccaagat cggccccgac gcaaagtccg ccctgccggc gatcgaaaag tgccgcagcg
     2221 atgccgaccc ggtcgtgcgc cagctcgccg aagccgcgat caagcggatc acgatgtgac
     2281 gccggcccgc ccgatcactt cgaccagcac gctcttgtcg gcgctcgctt tcgccgccga
     2341 acgcacttcc aagcggaggc cgtaggtctc cgctgtcgta attcgcacgg cgatgtcgtc
     2401 cgcatcgatc gcatgagccg tcagctccgg cggcgacgcg agcggcgggc agaacgccag
     2461 gtgcacaacg gcctgggtgt cgcccgcagg aattcgggcc cgcaccaggg cgtgcagggc
     2521 ctcgccttcg ccctcgcgcg tccgcgtgag ctgctggacg atgttggccg ggatcgattc
     2581 gtcgggctcg gcgactggcg tctgctccgt ttcccagcgg cttcgtgcgc gagcggcgga
     2641 tcggcggcgt gagagcacga gccaactcgt cccctcactg gcgaccagca ggaaccaggc
     2701 gatcgcgacg gcccacgccg gcgcctcgcg tgatgtgagc gacgccagaa agaccgtagc
     2761 ggcaaggctg ggcactacaa catgtgccgc gagccgtagc agcgacgcat ggaacggatg
     2821 ctcgccagga tttgctgcca agtactgagt acttagtact gagtactcag tactggatgt
     2881 cttaggcaac gcagatccac gacgcgccgc taacgccagc actcgcccca ctgccgccag
     2941 cccgcccacc gcaacggccg ccgcaatcaa tcccgcaccg cccagcggca ccgcgagagc
     3001 tcccgacagc cgtcgcgtgc agaacaagac ggccagaaag accagcagcc cgagcgccgt
     3061 cagcccgagt gcctggcgaa gcgccagcgg gccgattccg atccggccag ttcgccagcg
     3121 accgattgct cccgccgtgg agcccatgcg atttcggcag gctggaagcc ttgccccaca
     3181 cggttagcgt gctgccagtg tcgcccctgc ggcttcggcc agggcggcgg ccttgtcggt
     3241 gctttcccag cggaaatcgg ggtcgctgcg gccaaaatgc ccgccggcgg cggtcttttg
     3301 atagatcggc cgccgcaggt cgagatagtt gatgatgccg cgcggcgtga gcgggaacac
     3361 ctgccgcacc acctcgcaca gccgttcgtc gtccacgcgg ccggtcccct gcgtatcgac
     3421 gtggacgctc accggatcgg caacgccgat cgcataggcg agctgcactt cgcaccgctc
     3481 ggccagttcg gccgcgacga tgttcttggc cacgtgccgg gccatgtagg cggcgctgcg
     3541 atcgaccttc gtcgggtcct tgccgctgaa cgccccgccg ccgtgccgcc cccagccgcc
     3601 ataggtatcg acgatgatct tccgccccgt caggccgctg tcgccgtgcg gaccgccgac
     3661 gatgaattgg ccggtcgggt tgatgtgata tttgatgtcg ccgctcacca gctccttcgg
     3721 caactccggg ccgataatct tgccgacgac gaagtcgtaa atctgtttct gcgtgacgtc
     3781 cggagcgtgc tgcgtcgaga cgacgaccgt gtcgattcgc accgcccggt cgccgtcgta
     3841 ttccaccgtc acctggctct tgctgtcggg ccgcagccac ttcacttcgc ccgcctgccg
     3901 ggctttcgtc agccggttca gaatgcggtg ggccagcgcg attggcagcg gcatcaattc
     3961 gggcgtctcg tcgcacgcat agccgaacat cagcccctgg tcgccggcgc cgtccttatc
     4021 gacgcccatg gcgatgtccg ggctttggct gtgcagcttg accagcacct cgcacgtgtc
     4081 ggcgcaaaag ccgatatcgt tgtccgtata gccgacctcg cggatgacgc ggcgggcgac
     4141 tttctcgtaa tcgacctggg ccttg
//
LOCUS       DASUGB010000981        12228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1360247, whole genome shotgun sequence.
ACCESSION   DASUGB010000981 DASUGB010000000
VERSION     DASUGB010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12228
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360247"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFV87_18560"
     CDS             <1..525
                     /locus_tag="VFV87_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5105830.1"
                     /translation="VKNYLKGKAEVLRPAENCQHTGHGLARIKTWLGDGKWDVIHFNF
                     GLHDLKFVDNEGKNAPPDKGHPQVSAEEYQKNLEALVARMKKSGAKLIFATTTPVPEG
                     AQARIAGDEKRYNAIAAAVMKQNGVAIDDLYAFALPKLAEIQLPANVHFKPEGSKELA
                     KQVAGEILKALETK"
     gene            589..1398
                     /locus_tag="VFV87_18565"
     CDS             589..1398
                     /locus_tag="VFV87_18565"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5105831.1"
                     /translation="MPTRRRFLQTTAAATASLPLAPAFAYAAPSRDAWIELFDGQTLK
                     GWHKNPTRIGHGDGGRWQVEEGTITGEQDPPGSGNGGILLTDRKFGDFELLIDLKPDW
                     GICSGLFLRGNDKGQCLQMMVDYHDAGNVGQLYGEGLGGFNTRTFDVNGKLEGEKLAA
                     FTTDKHHTAAEVGLVSACTPEEWIKAWKIDDWNTARVIVSGKYAHITTYLNDQQVCVF
                     DGSTSTAAKYDKDKVVEQLGAEGSIAVQVHGGKNWPSGAKCRWKNIKIKPA"
     gene            1509..2696
                     /locus_tag="VFV87_18570"
     CDS             1509..2696
                     /locus_tag="VFV87_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105832.1"
                     /translation="MRWYFADPANPLEAAHKQRTTAAMDRWWQAFQAKAKDLSALFSR
                     KSEWDIVSWMQDYLGSVDPRLCWEYGPAIRQEGHRLVITPESKFWLRPMVRTLLERAP
                     QVPGWEIYPYRLPEDASQTIRAVQGRVGVNITGALVQAQMTDRRKIELLWGFPSLPGA
                     DRDLLMNAAFVASEVLMGEQVLDTWIGVIDLLDGQNVGQTRPLPMERAQATVGALIRS
                     AHEQLPATRAIDISFEEGWYNVQLEPEPAEDYLRRDDLLVASTHAIEVFQAAHSSGIF
                     VSSCHSRMGEYFCYLKLDADEVPSEHRVEFRSQFEDALNPQLLQAGVGGCIGGGSGLR
                     YSYIDLALTDVNRAVPIIRQVLAHHQAPLRSWLLFFDDELAREWVGVYQHTPEPPLPA
                     EEE"
     gene            complement(2736..3989)
                     /locus_tag="VFV87_18575"
     CDS             complement(2736..3989)
                     /locus_tag="VFV87_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5105833.1"
                     /translation="MRCHLSRLALSAAVVFAITASTCGHPLAEWPQFLGPSRNGNSAD
                     MGLLEKWPAGGPKELWRVAGGIGMSGLAIKDGKLLTLVQRDGQQWVVAHDAKTGAAQW
                     QTPVASEYENQMGNGPRATPAIAGDRAFVFTGEGILAAVALADGRLLWSHNVLKELKG
                     KEADYGMASSPLVVGDAVIVIAGAPNATIAAYNVQSGKLAWIAGDDPAGYSSPALLKL
                     GGKEQIVAYTGAAAIGLSPQGALLWRYPYETDFNCNIATPLAVDGKVFLSSGENHGSV
                     LLSLESDGEEFAAAEIWASQGPQSVLRNEWQTSILRDGHLYGFDNVGSAGPVTHLTCI
                     EAATGKRVWQQTRFGKGNLIAADGKLFISTMKGELVVVRATPDKYDELGRATVIGSTR
                     QAPSLAGGLLYLRDDKEIVCLDVRK"
     gene            4190..5365
                     /locus_tag="VFV87_18580"
     CDS             4190..5365
                     /locus_tag="VFV87_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5105834.1"
                     /translation="MSPAPELHPPVRVLMGPGPSDTHPRVLQALAKGTVGHLDPYYLE
                     VMNGLQEMLRGLFKTKNPMTMAISGTGSAGMEAAVVNLIEPGDSMVVCVNGVFGARMA
                     DVAERAGAKVTKVEKPWGEVYSPNDLKAALAAKPKVVGIVMAETSTGARQPIPEISKA
                     VHDAGAMLLVDAVTALGGIPVEVDAWNIDAIYSGTQKCLSCPPGLSPVSFSPRAMETI
                     QSRKTKVQSWYLDVSMLANYWGQNRVYHHTGPINMTYALYEALRLIHEEGLENCHARH
                     LRNHQALKAGLAALGIQYATAEHCTLPQLNAVLVPAGVDDAAVRSGLLNRFGIEIGAG
                     LGAYKGKVWRIGLMGFGSRPANVLLFLSALEQLLAEQGLKFEHGASIAAANASYAGK"
     gene            5388..7946
                     /locus_tag="VFV87_18585"
     CDS             5388..7946
                     /locus_tag="VFV87_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HEX5105835.1"
                     /translation="MSANPPSRPELLAPAGDRECIRAAIENGADAVYFGLDAGFNARA
                     RAKNFDPAELPEIMAELHRRGLKGYVTLNTLVFPSELPAVEQLVRQVAAAGVDAVLVQ
                     DLGLVRLIREICEDLPIHASTQMTLTSSQSIAAALELGASRVVLARECSLKEIRRIAG
                     ETTMPLEVFVHGALCVAYSGQCLTSESLGGRSANRGQCAQACRLPYDLVCDGRDIELG
                     EQKYLLSPQDLAAYDLIPDLIAAGVCSFKIEGRLKTPEYVANITRHYRQAIDAALAGS
                     QAQFTPRDVEEMELSFSRGFSPGWLGGCDHKMLVPAISSAKRGVLIGIVRAVRNGRVV
                     LEGTGRHVKRGDGVSFDSGQAVDDEQGGRVYDVAIHGDALELAFGHGAIDFAQIQPGM
                     RVWKNDDPELNGRLRKSFTAADPQRRTAVDLEIRAAARQPLHITVSTETGLQFEFAGQ
                     QPCAEAQKHPLTAESLREQFRRLGGTPFTLRELTAKIEGRPMLPFSILGQLRHELVRR
                     LEEQSTTSAPRRIAAEPVLPRLRAAIVARSASEGSAGGMPITLACATGYDPLAAAPQA
                     APTLHVLCRSLAQLRDVLAAGITSLYAEFQDIREYREAVRIACERQAAIFLATPRIQK
                     PDEMGIFHALAKHRADGIVVRNLAGLRFYRERGIPFVADFSLNTANDLTFQYLIEQGA
                     ARVTPSYDLNREQLLELAHAVPTERLEVVVHQHMPMFHMEHCVFCAVLSPGTNKTNCG
                     RPCDTHEVQLRDRIGMQHPLKADVGCRNTLFNAVPQSGAEIVPQLLAAGVRHFRVELL
                     DAAGDDVAAVLDLYRLLLAGDVTGKEVWTRLKAANRVGVTRGTLEERRNPLAIV"
     gene            8013..8534
                     /locus_tag="VFV87_18590"
     CDS             8013..8534
                     /locus_tag="VFV87_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105836.1"
                     /translation="MSSDPQGMSSDTKKLIPILIIIGVLMCGCIAVPVVGGIAMLGFV
                     SARHDAAAHEALQQADAVDFTMPGEIAPPMIPQLPQPIGEFSPSGGGKEAAQARMLAA
                     EADLQLAQAQYNAAQAVYEQQRNFANAQAGRLGAQGVSIPPPQPPDPSLYQAVLAAQQ
                     NYDAAKAEYEAFP"
     gene            8658..9725
                     /locus_tag="VFV87_18595"
     CDS             8658..9725
                     /locus_tag="VFV87_18595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX5105837.1"
                     /translation="MHSPLGKLAVTLAAIACLPGLTRADDLADARDALEKLGLRAIST
                     AVLLPAESDLAKELSKSALLRKNLTLAAKDQQNVENQAILLQKTLTGLRQQHVVLNAQ
                     LANVNPNDVTTNNRLVGALNAIAGQFDLLKEKQGEMEEAQTAARAKVNEAREAYVQMV
                     LSSRKLADQIAADYELKAVDPAVKAAIDKLNAATGKPVALGPSTAFAGNVRRLKALED
                     TVLSESIPLRDSGGGTFHVSVVVNGKEQQEMVLDSGASLISLPLALAERFGLKPSDKD
                     PTIVLQLADGREIEGRLMKLKSVRVGKFSVEDVECAVLGEDAIRAEPLLGMSFLGNFK
                     FEVDATAKELTMVKVAGADGK"
     gene            9890..10774
                     /locus_tag="VFV87_18600"
     CDS             9890..10774
                     /locus_tag="VFV87_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5105838.1"
                     /translation="MITPNRRQFLQTTAALGTAAVLPAAARAAAEPPAEAAIAFGLVT
                     YMWGADWDLPTLLENCAKTNCKGVELRTTHAHKVEPDLSDKQRAEVAARFADAGVTCV
                     GIGSNERFDDPNPEVVKKAIEASKQFVRLSHDIGGSGVKVKPDRFWPDVPREKTIEQI
                     GKALNELGEYAAGFGQQIRLEIHGQCGELPTIKAIMDVATDDNVALCWNSNKTDLAGD
                     GLAANFQLVRPRFGQTCHIHELESPDYPHQDLLGLLVKSKWEGWLLMENATKPADRVA
                     AMAHQRKLFDEMLKRAQA"
     gene            10892..>12228
                     /locus_tag="VFV87_18605"
     CDS             10892..>12228
                     /locus_tag="VFV87_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105839.1"
                     /translation="MPDDQQGLFPELQPARNGNGRKGQRLFIPKLLTDASRSTWLNDR
                     IDEAHAIFVKWADLETSGKLASKSEKTLHGEFFADVLKGALGYTQFSDGLENWDLEAE
                     LAVAGGHADGAIGLFSAADRPPPRVLLELKGPTCNIDRDRSNGRTPVQQVWDYLNDTP
                     ACPWGIVCNYVSFRLYHRTKTTRTYEHFTLQELREKSKFREFYCLFERGGFLPALAGQ
                     KPRCDDLLERSEHQQQQVGGKLYNFYHDQRIGLIHCLRRPPHKLPLDQAIHAAQKLLD
                     RIIFIAFCEDRKLLPSGIIEYAWDIKGITDYENPRWRSFLGLFKRVDKGDSRLLITGY
                     NGELFKHDLLLDKLELDDEWTNVFKEIADYDFDTEVNVEVLGHLFEQSITDLELLREN
                     PDAEPAQVTQRRPGRRKREGVYYTPPFITAYIVRQAIGPCLQERYAALATQLKL"
BASE COUNT         2384 a         4037 c         3813 g         1994 t
ORIGIN      
        1 gtcaagaact acctgaaggg gaaggcggag gttttgcgcc ccgctgagaa ctgtcagcat
       61 accggccacg ggctggcgcg gatcaagacc tggctcggcg acggcaagtg ggacgtgatt
      121 catttcaact tcggcctgca cgatctgaag ttcgtcgata acgagggcaa gaacgcgccg
      181 cccgacaaag gtcatcccca ggtgagcgcc gaggagtatc aaaagaacct cgaggcactg
      241 gtcgcccgca tgaagaaatc gggggccaag ctgatcttcg cgaccaccac gcccgtcccc
      301 gaaggggccc aggcgcggat tgcgggagac gaaaagcgat acaacgcgat tgccgcagca
      361 gtgatgaagc agaacggcgt ggccatcgac gacctatatg ccttcgctct gccgaagctg
      421 gctgagattc aattgccggc gaacgttcac ttcaagccgg agggctcgaa ggaactggcc
      481 aaacaggtcg ccggtgaaat cctgaaggcg ctcgaaacga aataggtcaa gccagatcca
      541 ctcacagtcc ttcgggagca agtgtgctcc cggccaggag cattgatcat gcccacccgc
      601 cgccgattcc tccaaacaac cgctgccgcg acagcttcgc tgccactggc ccctgcattc
      661 gcctacgccg ccccttcgag agacgcgtgg attgaactat tcgacggcca gacgctgaaa
      721 ggctggcaca agaatccgac caggatcggc cacggcgacg gcggccgctg gcaggtggaa
      781 gagggcacaa tcaccggcga gcaggacccg cccggcagcg gcaacggcgg cattctgctg
      841 acggaccgca aattcggcga tttcgagctg ctgatcgacc tcaaacccga ttggggcatc
      901 tgctccggct tgtttctccg cggcaatgac aaggggcaat gcctgcagat gatggtcgat
      961 tatcacgatg cgggaaacgt cggtcagctt tatggcgaag ggctgggcgg cttcaatacg
     1021 cgcacgttcg acgtcaacgg caagctggag ggcgagaagc tggctgcgtt cacgaccgac
     1081 aagcatcaca cggctgccga agttggtctt gttagcgctt gcacccccga agagtggatc
     1141 aaggcctgga agatcgacga ctggaatacg gcacgcgtga tcgtcagcgg caaatatgcc
     1201 cacatcacga cgtacctgaa cgatcagcag gtttgcgtgt tcgacggcag cacgtcgact
     1261 gccgccaaat acgacaagga caaggtcgtg gagcagctcg gcgcggaagg gtcaatcgcc
     1321 gtgcaggttc acggcgggaa gaactggcca tccggggcca agtgccgctg gaagaacatc
     1381 aagatcaagc cggcctgatt tggggcagac atccttatct gccaccatca aattcgcagc
     1441 aaccgttcca cgacaggcca atcaagggct ttagctacgg cagacaagaa tgtctgcccc
     1501 acttgcttat gcgttggtac ttcgccgatc ccgccaatcc gctcgaagcc gctcacaagc
     1561 agcggacaac cgctgcgatg gatcgttggt ggcaggcgtt ccaggcgaaa gccaaagacc
     1621 tttccgccct cttttcgcgg aagagcgagt gggacattgt ctcctggatg caggactacc
     1681 tcggcagcgt cgatccgcgg ctgtgctggg aatatggccc ggcaatccgc caggagggac
     1741 accggctggt gatcacgccg gaaagcaagt tctggttgcg gccgatggtg cgaaccttgc
     1801 tggagcgagc gccgcaggtt cccggctggg aaatctatcc ctaccggctg ccggaagatg
     1861 catcgcaaac aattcgtgcg gtgcaaggcc gcgtgggcgt aaacatcacc ggagcgctcg
     1921 tgcaggcaca gatgacggac cgccgaaaga tcgagctgct ctggggcttc cccagcctcc
     1981 cgggtgcgga ccgcgatctg ctgatgaacg cggcgttcgt cgcctcggaa gtgttgatgg
     2041 gggaacaggt gctcgacacc tggatcggcg tcatcgatct tttggacggt cagaacgtcg
     2101 gccaaacgcg gccgctgccg atggagcggg ctcaagcgac ggtcggcgcg ctcattcgca
     2161 gcgcccacga gcagttgccg gccacgcggg ccatcgacat cagcttcgag gagggctggt
     2221 acaacgtaca actcgagccg gaaccggcgg aggactatct tcgccgcgac gacctgctcg
     2281 tcgcctcgac gcacgcgatc gaggtcttcc aggcggctca cagcagcggg atcttcgtct
     2341 cgagctgtca ttcgcggatg ggcgagtact tctgctacct caagctcgat gccgacgaag
     2401 tgccgagcga acaccgcgtc gaattccgct cgcagttcga ggacgcgctc aatccgcagc
     2461 tcctgcaggc cggggtcggc ggctgcatcg gcggcggcag cgggctgcgc tattcgtata
     2521 tcgacctggc attgaccgac gtcaaccgcg ccgtaccgat catccgacag gtcctcgcgc
     2581 accaccaggc gcccttgcgt agctggctgc tcttttttga cgacgagttg gcgcgggaat
     2641 gggtcggcgt atatcagcac acgcccgaac cgcccttgcc agcagaggaa gagtaacgag
     2701 tggcagacat tctagtccgc cacaatccac gtgacttact tccttacgtc cagacacacg
     2761 atctccttgt cgtcgcgcag atacagcagg ccgccggcca gggatggcgc ctggcgggtg
     2821 ctgccgatga cggtcgcccg tccgagttcg tcgtatttgt cgggcgtggc gcggacgacg
     2881 accagctcac ctttcatggt ggaaatgaag agcttgccgt cggcggcgat gagattccct
     2941 ttgccgaagc gagtctgttg ccagacgcgt ttgccggtgg cggcttcgat gcaggtcaga
     3001 tgcgtcaccg gccccgcgct gccgacgttg tcaaagccgt acaggtggcc gtcccggagg
     3061 atcgacgtct gccactcgtt ccgcagcacg ctctgcggcc cctggctggc ccagatttcg
     3121 gcggcagcga actcttcgcc atctgactcc agggaaagca gaacgctgcc gtgattctcg
     3181 ccggaggaca ggaaaacctt gccatcgacc gccagcggcg tagcgatatt gcagttgaag
     3241 tccgtttcgt agggatagcg ccaaagcaga gcgccctgtg gtgaaaggcc aatcgccgcg
     3301 gcgccggtgt aggcgacgat ctgctccttg ccgccgagtt tgagcagcgc cggcgacgaa
     3361 taacccgcgg ggtcgtcacc cgcgatccag gcaagttttc cgctctgcac gttgtatgcc
     3421 gcgatcgtgg cgttcggcgc tccggcgatg acgatcaccg cgtcgccgac aacgagcggc
     3481 gagctggcca taccgtaatc ggcctctttg cccttcagtt cctttaagac gttgtgcgac
     3541 cagagcagtc tgccgtcggc cagagcaacc gccgccagga ttccttcgcc ggtgaagaca
     3601 aaggcccgat ccccggcaat cgccggcgtt gcccgcgggc cgttgcccat ctgattctcg
     3661 tattcggacg ccacgggcgt ctgccactgc gctgcgccgg tcttggcgtc atgagccacg
     3721 acccattgct gaccgtcgcg ctgcaccagg gtcagcagct tgccgtcctt gatcgccagc
     3781 cccgacatac cgatgccgcc ggccacccgc cagagctcct tcggtcctcc cgcaggccac
     3841 ttctcgagca agcccatgtc agccgaattg ccgttgcggc tggggcccaa aaactggggc
     3901 cattcggcga gcgggtgacc acaggtcgaa gccgtgatgg cgaaaacgac tgccgcgctg
     3961 agcgcaagcc gtgacaggtg acaacgcatc gatccgatcc ttctccaaaa agggaccgct
     4021 acactccagg cgtatgatac gcccgatagg acggcaatct caatgcacgt ttgatggctg
     4081 gtgtagcgga acgcacaacg gtagaacaat cgggcgagcg cgacgatgat tcgacacgaa
     4141 accagaactg ctttcactca ggaacgcgat caccgatcgc ggctttgaaa tgtcacccgc
     4201 tcccgaactt cacccgcccg tccgcgtcct catgggaccc ggcccgagcg atacgcatcc
     4261 ccgcgtgctg caagcgctcg ccaaggggac ggtcggacat ctcgatccgt attacctgga
     4321 ggtgatgaac ggcttgcagg aaatgcttcg cgggctcttc aagacgaaga acccaatgac
     4381 gatggcgatc agcggcacgg gctcggcagg gatggaagcg gcagtcgtca atctgatcga
     4441 gcccggcgac tcgatggtgg tgtgtgtgaa cggcgtcttc ggcgcgcgga tggccgacgt
     4501 cgccgagcgc gccggggcga aggtgacgaa ggtcgaaaag ccgtggggcg aagtctattc
     4561 gcccaacgat ttgaaggcgg cgctggcggc aaagcccaag gtcgtcggca tcgtgatggc
     4621 cgaaacctcg acgggcgccc ggcagccgat tccggagatc tcgaaagccg tccacgacgc
     4681 gggggcgatg ctgctggtcg atgcggtcac ggctctcggc ggcattccgg tcgaggtcga
     4741 tgcctggaac atcgatgcca tctacagcgg cacgcagaag tgcctctcgt gcccacccgg
     4801 cctgtcgccc gtctcgttca gcccgcgggc gatggagacg atccagagcc gcaagacgaa
     4861 agtccaaagc tggtatctcg acgtctcgat gctcgccaac tattggggcc aaaaccgcgt
     4921 ctatcatcac accggcccga tcaacatgac ctacgccctg tatgaggcgc tgcggctgat
     4981 ccacgaagag gggctggaga attgccacgc ccggcatctg cggaaccacc aggcgctcaa
     5041 agcgggcctg gccgcgctcg gcattcaata cgccaccgcg gagcattgca cgctgccgca
     5101 gctcaacgca gtgctggtgc cggcgggcgt cgatgatgcc gccgtgcgct ccgggctgct
     5161 caaccgcttc ggcatcgaga tcggcgcagg gctcggtgcc tataaaggca aggtctggcg
     5221 aatcggcctg atgggctttg gctcgcggcc ggcgaacgtg ctgctgttcc tgtcggcact
     5281 cgagcagctc ctggccgagc aggggctcaa gttcgagcac ggagcaagca ttgctgcggc
     5341 gaatgcctct tacgccggaa aataggccgc cgtaggatgg acggacgatg tccgccaacc
     5401 ctccatcccg ccccgaattg ctcgcccccg ctggcgaccg ggagtgcatt cgcgcggcga
     5461 tcgaaaatgg agcggacgcg gtctatttcg ggctggatgc aggcttcaac gcccgggccc
     5521 gggccaagaa cttcgatccg gctgagctgc cggagatcat ggcggagttg caccgccgcg
     5581 ggctgaaggg ctacgtcacg ctcaacacgc tcgtctttcc gagcgagctg ccggcggtcg
     5641 agcaactggt gcggcaggtg gccgcggcgg gcgtcgatgc ggtcctggtg caggatttgg
     5701 gcctcgttcg cctcatccgc gaaatctgcg aggatttgcc gatccacgcc agcacgcaaa
     5761 tgacgctgac cagtagccag tcgatcgcgg ccgctttgga attgggcgcc agccgcgtcg
     5821 ttctggcccg cgagtgctcc ctcaaggaaa tccgccggat cgccggcgaa acgacgatgc
     5881 cgctggaggt gttcgtgcac ggcgcgctgt gcgtcgccta ttccggtcag tgcctgacca
     5941 gcgagtcgct cggcggccgc agcgccaacc gcggccagtg cgcccaggcc tgccgcctgc
     6001 cgtacgatct ggtctgcgat gggcgggaca tcgagctggg cgagcagaag tacctgctca
     6061 gcccgcagga cctggcggct tacgacctga tccccgacct gatcgcggcc ggcgtgtgca
     6121 gcttcaagat cgaaggccgg ctgaagacgc cggagtacgt cgcgaacatc acgcggcatt
     6181 accgccaggc gatcgacgcg gccctcgccg gcagccaagc gcaattcacg ccccgcgacg
     6241 tcgaggaaat ggagctgtcg ttctcgcgcg ggttctcgcc cggctggctg ggcggctgcg
     6301 atcacaagat gctcgtgccg gccatcagct cggcaaaacg cggcgtgttg atcggcatcg
     6361 tgcgagccgt gcggaatggt cgagtcgtcc ttgaaggcac aggtcgccat gtgaagcgcg
     6421 gcgatggcgt ctcattcgat agcggccagg ccgtggatga cgagcagggc ggtcgggtgt
     6481 atgacgtggc gattcacggc gacgcgctcg agttggcgtt cggccacggc gcgatcgact
     6541 ttgcgcagat tcagcccgga atgcgcgtct ggaaaaacga cgatccggag ctgaatggcc
     6601 gcctgcggaa gtcgtttacg gccgccgatc cgcagcggcg gacggcagtc gatctggaga
     6661 tccgcgccgc cgcccgtcag ccgctgcata ttaccgtttc gaccgagacc ggcctgcaat
     6721 tcgaattcgc cggccagcag ccctgcgccg aagcccagaa gcatccgctc acggctgaat
     6781 cgctccgcga gcagttccgt cggctgggcg gcacgccgtt caccctccgc gaattgaccg
     6841 ccaagatcga aggccggccg atgctcccct tcagcatcct cggccagttg cggcacgagc
     6901 tggtgcgacg tctggaggag cagtcaacga caagcgcccc gcgtcgaatc gccgccgaac
     6961 ccgtcttgcc gcggctgcgg gccgccatcg tagcccgcag cgcaagcgag ggtagtgcgg
     7021 gtggaatgcc gattaccctc gcttgcgcta cgggctacga ccccctcgcg gccgcccccc
     7081 aagcagcccc aaccctccac gtcctctgcc gctcgctcgc ccagcttcgc gacgtgctcg
     7141 cggccggcat caccagcctg tatgcagaat tccaggacat ccgcgagtac cgcgaagcgg
     7201 tcagaatcgc ttgcgagcgg caagcggcca tcttcctcgc cacgccgcgg atccagaagc
     7261 ccgacgaaat gggcatcttc cacgcgctgg ccaaacaccg cgcggatggc atcgtggtcc
     7321 ggaatctcgc cggcctccgg ttctatcgcg agcgggggat acccttcgtc gccgatttct
     7381 cgctaaacac ggccaacgac ctgacgtttc aatacctgat cgagcagggc gcggcccgcg
     7441 tcacgccgtc gtacgacctc aaccgcgagc agctcctgga actcgctcac gccgtgccaa
     7501 ccgagcggct ggaagtcgtc gtccaccagc acatgcccat gtttcacatg gagcactgcg
     7561 tcttttgcgc agtcctctcg cccggcacca acaagaccaa ctgcggccgg ccctgcgaca
     7621 cccacgaagt ccagctccgc gaccgcatcg gcatgcagca tccgctcaag gctgacgtcg
     7681 gctgccgcaa tacgctgttc aatgccgtgc cgcaaagcgg ggcggagatc gtgccgcagc
     7741 ttctggccgc cggcgtgcgg catttccgcg tggaattgct cgatgcggcc ggcgacgacg
     7801 tggctgccgt gctcgacctc taccgcctcc tcctcgcggg cgacgttacc ggcaaggaag
     7861 tctggacgcg cctcaaggcg gccaaccgcg tcggcgtcac gcgcggcacg ctggaagagc
     7921 gccgcaatcc gctcgccatc gtgtagttgc cttccggcgg ccgagttatg atgttccctg
     7981 gctggcgcta acatccgtaa cctggaggac ctatgtcgtc cgatccccaa ggcatgtcgt
     8041 ccgatacgaa aaagctcatc cccatcctca tcattatcgg cgtgctgatg tgcggctgca
     8101 tcgccgtgcc ggtcgtcggc ggcatcgcga tgctgggctt cgtcagcgcg cgtcacgatg
     8161 cagccgccca tgaagccctg cagcaagccg acgcagtcga tttcaccatg ccgggcgaaa
     8221 tcgcgccgcc gatgattcca cagctcccgc agccgatcgg cgaattcagc ccctccggcg
     8281 gcggcaagga agcggcccag gcccggatgc tcgcggcaga agccgacttg cagcttgccc
     8341 aggcccaata caacgccgcc caggccgtgt acgagcagca gcggaacttc gccaatgccc
     8401 aggccggccg cttgggcgcg cagggcgtct ccatcccgcc accccagcct cccgatccgt
     8461 ccctctacca ggccgtcctc gccgcgcagc aaaactacga cgccgccaaa gccgagtacg
     8521 aagcctttcc ctaggcgtga gatttcactg gctttttcgc cggagcaaac atacaaccgt
     8581 tagtcgcctt tggctccgcg aaagggcgtt cgttcgcgga ccgaacgacg acaccaccaa
     8641 ttccggaggg atcacgcatg cactcgccgc ttgggaaact cgccgtcacc ctcgccgcca
     8701 tcgcctgcct gccgggactg acccgggccg acgacctggc cgacgcccgc gacgccctcg
     8761 aaaagctcgg cctgcgggcc atttcgacag ccgtcctgct gccggctgaa agcgacctgg
     8821 cgaaggagct ctcgaaatcg gccctgctgc gaaagaacct cacgctcgcg gcgaaggacc
     8881 agcagaacgt cgaaaaccag gcgatcctgt tgcaaaagac gctcaccggc ctccgccagc
     8941 agcacgtcgt gctcaatgcc cagctcgcga atgtcaatcc gaacgacgtg acgaccaaca
     9001 accgcctcgt cggcgcgctc aacgccatcg ccggccagtt cgacctgctc aaggaaaagc
     9061 aaggcgagat ggaagaggcc cagaccgcgg cccgggccaa ggtgaacgaa gcccgcgagg
     9121 cctacgtgca gatggtcttg agcagccgca agctggccga ccagatcgcc gccgattacg
     9181 agctgaaggc ggtcgatccg gctgtcaaag cggccatcga caagctgaac gccgccaccg
     9241 gcaagccggt cgccctcggc cccagcaccg ccttcgccgg caacgtgcgg cggctgaagg
     9301 ccctggaaga cacggtcctc tccgaatcga tcccgctgcg cgattccggc ggcggcacgt
     9361 ttcacgtcag cgtcgtcgtc aacggcaaag agcagcaaga gatggtgctc gattccggcg
     9421 ccagcctgat cagcctgccg ctggccctgg ccgagagatt cggcctcaag cccagcgaca
     9481 aagacccgac gatcgtgctc cagcttgccg acgggcggga aatcgaaggc cgcctgatga
     9541 agctgaagtc ggtgcgggtc ggcaagttca gcgtcgaaga cgtcgaatgt gccgtcctcg
     9601 gcgaagacgc catccgggcc gagcccctcc tgggcatgag cttcctcggc aatttcaagt
     9661 tcgaagtcga cgccaccgcc aaagagctga ccatggtgaa agtcgccggg gcggatggca
     9721 agtagccacg gaccaaacac ggatgaaacc cagcgcggcc cagccgtaac caaatccgcc
     9781 aggcgggagc cacagatttg cacagatttg cacggataag gcacgaggta tgtctgatcc
     9841 gcgtcatccg tgtcatccgc ggcttccttt ccctatcgag gacagttcca tgatcacccc
     9901 caatcgccgc caattcctgc aaaccaccgc tgccctgggc acagccgccg ttttgcccgc
     9961 cgcggcgcgg gccgccgccg aaccgccggc ggaggcggcg atcgccttcg gcctggtgac
    10021 gtacatgtgg ggggcggatt gggatttgcc gaccctcctg gagaactgtg ccaagacgaa
    10081 ctgcaagggc gtggaattgc ggacgacgca cgcccacaag gtcgagccgg acttaagcga
    10141 caagcagcgg gccgaagtgg ccgcccgctt cgccgatgcg ggggtcacct gcgtcggcat
    10201 cggcagcaac gagcggttcg acgaccccaa ccccgaggtc gtgaagaagg cgatcgaggc
    10261 gagcaagcag ttcgtccgcc tcagccacga catcggcggc agcggcgtga aggtcaagcc
    10321 cgaccgcttc tggccggatg tgccccgcga aaagacgatc gagcagatcg gcaaggcgct
    10381 caacgagctg ggcgaatacg ccgccggctt cgggcagcag atccgcctgg aaatccacgg
    10441 ccagtgcggc gagctgccga cgatcaaggc gatcatggac gtcgcgacgg acgacaacgt
    10501 ggccctctgc tggaacagca acaagaccga cctggccggc gacggtctgg cggcgaactt
    10561 ccagctcgtc cgcccgcgct tcggccagac ctgccacatc cacgagctcg aatcccccga
    10621 ctacccccac caggacctgc tgggcctgct cgtcaaatcc aaatgggaag gctggctcct
    10681 catggaaaac gccaccaaac ccgccgaccg agtcgccgcc atggcccacc agcgcaagct
    10741 cttcgacgag atgctgaagc gggcgcaggc gtaggtaggg cccggccccg ccacggcagc
    10801 gcgttgcgtg ccacggcgaa cctgttatcc tgctctcctc ggacgaattc tctcccaagg
    10861 acggccagga ccactgggcc gcaacatcac ggtgccggac gatcaacagg gactgtttcc
    10921 agagctccag ccggcccgca acgggaacgg acgcaaaggg cagcggctct ttatcccgaa
    10981 gctgctgacc gatgcctcaa ggagcacctg gctgaacgac cggatcgacg aagcccatgc
    11041 gatcttcgtc aaatgggccg atctggagac gagcggcaag ctagccagca agtcggaaaa
    11101 gacgctccac ggcgaattct tcgcggacgt gctgaaagga gcgctcggct atacgcagtt
    11161 ttccgatggc ctggaaaact gggacctcga agccgagttg gcggtcgccg gcggccatgc
    11221 cgacggggcg atcggcctgt tctcggctgc cgatcgcccg ccgccgcgcg tgctcttgga
    11281 gctgaaaggc ccgacgtgca acatcgaccg cgatcgatcg aacggccgca cgccggtgca
    11341 gcaggtctgg gactatctca acgacacccc cgcctgtccc tggggcatcg tctgcaacta
    11401 cgtcagcttc cggctctacc accgcacgaa gaccacgcgg acttacgagc atttcacgct
    11461 gcaggagctg cgcgagaaga gcaagtttcg cgaattctat tgcctcttcg aacgcggcgg
    11521 cttcctcccc gccctcgccg gccaaaaacc gcggtgcgat gacctgctgg agcggagcga
    11581 gcaccagcag cagcaagtcg ggggcaagct gtacaacttc taccacgacc agcggatcgg
    11641 cttgattcat tgcctccggc ggcccccaca caagctgccg ctggaccagg cgattcacgc
    11701 cgcgcaaaaa ctgctcgacc ggatcatctt catcgccttt tgcgaagacc gcaagctgct
    11761 cccgagcggg atcatcgagt acgcctggga tatcaagggg atcaccgact atgagaaccc
    11821 gcggtggcga agttttcttg gccttttcaa gcgcgtggac aagggcgatt cccgtctgct
    11881 cattactggc tacaacggcg agctgttcaa gcacgacctg ctgctggaca agctggagct
    11941 ggacgacgag tggaccaacg tgttcaagga aattgccgac tacgacttcg acacggaagt
    12001 caacgtcgag gtgctcggcc acctcttcga gcagtcgatc accgacctgg agctattgcg
    12061 cgagaacccc gacgccgagc cggcgcaggt cacgcagcgg cggcccggcc gccgcaagcg
    12121 cgaaggggtc tattacacgc cgccgttcat caccgcctac atcgttcgcc aggcgatcgg
    12181 tccctgcctg caggagcggt atgccgcgct cgccacacaa ctgaagct
//
LOCUS       DASUGB010000982         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1639922, whole genome shotgun sequence.
ACCESSION   DASUGB010000982 DASUGB010000000
VERSION     DASUGB010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639922"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFV87_18610"
     CDS             complement(<1..617)
                     /locus_tag="VFV87_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5105840.1"
                     /translation="MGDAAYSKTSPLSPRHREADTSRSPPRSAYIHVPFCRHHCGYCN
                     FTLVAGRDDLIKAYLEALAKELSWLDSPNEVDTLFLGGGTPTHLPPDELVELLKLALR
                     WFPLAAEHELSVEANPVDLDDARCEVLVAAGVTRVSLGIQSFQAEKLQALERDHRQAE
                     IFAALAKARKLARSVSLDLIFAAPGETLKQWRQDLETAISLQPDH"
     gene            complement(664..1800)
                     /locus_tag="VFV87_18615"
     CDS             complement(664..1800)
                     /locus_tag="VFV87_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5105841.1"
                     /translation="MHIAAVESYPVRIPLKADHRMISALGRHDESKFVVVRVLSDTGL
                     EGAGEATATPRWSGETVWGVQAMIDRVLGPALIGMELSAGDPFVSIRAIDARMDALAI
                     HNWFAKSALEMACWDLLGKQAGKPVYELLGGECRSLVIPSRFSMGAYDLETAKRRAKE
                     LVAQGFDTVKVKVGGEVNQDIARVRTVHKEIGHERKLVIDANCGWSAETAIHALHELR
                     DCRVLIMEQPTPDGDYEAIARVRRETGVKVMADDMCFNLTHARELLRNEACDLISVYP
                     GKNGGIRKSREIVEFAARHDIGCTVGSNLEWDIATAAMGHLIVATPNLLVEEYPGDIH
                     GPAYYEFGIAKNPLRVDGPETEITDRPGLGVDVDWEVVRGNPCS"
     gene            complement(1820..>2838)
                     /gene="rlmN"
                     /locus_tag="VFV87_18620"
     CDS             complement(1820..>2838)
                     /gene="rlmN"
                     /locus_tag="VFV87_18620"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5105842.1"
                     /translation="LSSWSIAPIHAARVMKYIYREGAGSAQEMSELPASLRRRLAEEF
                     SWDDLEQCSEAVSSDGFTRKLLLGMADGQRVEAVLMQFRGRWTACVSSQVGCAMGCVF
                     CATAQMGLVRQLTTGEIVQQVVHANRLLHQPGLALRNVVLMGMGEPLHNYDAVMKAVA
                     ILCEPTGLALAPRHITLSTVGVIPGIVRLADERQRVNLAISLHAATQEERAKLVPPAR
                     AWPLDELMAACRYYAGKLQRRIFFEWALIDGQNDSAEHAAALGQLLDGLPSHVNLIPL
                     NPIAGYDGVPTPPASAKRFQAILAAHVIPSTIRQRRGIDIAAGCGQLAARNVPKPEAA
                     CLGD"
BASE COUNT          526 a          931 c          846 g          535 t
ORIGIN      
        1 atgtgatccg gctggagaga aatcgcagtt tccaggtcct gccgccactg cttcagcgtt
       61 tcgccgggag cagcaaagat gaggtccagc gagacggaac gggcgagttt tcgcgccttc
      121 gccagagcgg caaatatctc ggcctgtcga tggtctcgct ccagggcttg cagtttctcg
      181 gcctgaaagg actgaatccc caggctgacg cgcgttacgc cggcggcgac caggacttcg
      241 cagcgagcgt cgtccagatc aaccggattg gcttcaacgc tcagttcgtg ttcagccgcg
      301 agcgggaacc agcgcagcgc gagcttgaga agttccacga gctcatcagg cggcagatgc
      361 gtcggcgttc cgccgccgag gaacagcgtg tctacttcat ttggactatc cagccaggag
      421 agttctttcg cgagcgcctc caagtaggcc ttgatcaggt cgtcccgccc cgccaccagt
      481 gtgaagttgc aatagccgca gtgatgacgg cagaagggga catggatgta ggcggagcgg
      541 ggaggcgagc gggaggtgtc agcctcccgg tgacggggcg ataacggcga ggtcttgcta
      601 tacgcggcat ctcccattgg ccaacctacc ggagggctta cgccctccgc tcgccaagaa
      661 tacttacgaa cacggattgc cccgcaccac ctcccaatcg acatcgaccc ccagccccgg
      721 ccggtcggtg atttccgttt ccggcccgtc cacgcggagc ggattcttgg cgatgccgaa
      781 ttcgtaatag gcgggaccgt ggatgtcgcc gggatattcc tccacgagca agttcggcgt
      841 ggcgacaatc aggtgtccca tcgcggcggt cgcgatgtcc cattccagat tggagccgac
      901 ggtgcagccg atatcgtggc gggccgcgaa ctcgacgatc tcccgcgact tacgaatacc
      961 tccgtttttg ccgggataga cgctgatcag gtcgcacgcc tcatttcgca gcagctcgcg
     1021 ggcgtgcgtc aggttgaagc acatatcgtc ggccatcacc ttcacccccg tctcccgtct
     1081 tacgcgggcg atagcttcat aatcgccgtc cggcgtcggc tgctccataa tcaggacgcg
     1141 gcagtcgcgg agctcgtgca gggcgtggat cgccgtttcc gcgctccagc cgcaattggc
     1201 gtcgatgacc agctttcgct cgtggccgat ttccttatgg acggtgcgga cacgggcgat
     1261 gtcctgattg acttcgccgc cgaccttcac tttgacggtg tcaaagccct gggcgaccag
     1321 ctccttcgcg cgccgcttcg ccgtctccaa gtcgtaggcc cccatcgaga accggctggg
     1381 gataacgagc gagcggcact cgccgccaag cagttcgtag accggcttac ccgcctgttt
     1441 gcccagcaaa tcccagcagg ccatttcgag cgccgacttg gcgaaccagt tgtgaatggc
     1501 aagcgcgtcc atccgggcgt cgatagctct aatgctgacg aaaggatcgc cagcgctcag
     1561 ttccatgccg ataagtgccg gcccgaggac ccggtcgatc atcgcctgca cgccccagac
     1621 cgtctcgccg ctccagcggg gcgtcgccgt cgcttcgccg gctccttcca agccggtatc
     1681 ggaaaggact cgcacgacga caaacttcga ttcatcatgc cgccccaagg ccgagatcat
     1741 ccggtgatcg gctttgagcg ggatgcgaac gggatacgat tcgacggcgg cgatatgcat
     1801 gtctttatct tctgccgcat caatcgccaa gacaagccgc ctcaggtttc ggcacgttcc
     1861 tggccgccag ctgcccgcac ccggccgcga tatcaattcc ccgcctctgg cgaatcgtgc
     1921 tgggaatgac gtgcgcagcg aggatagcct gaaagcgttt ggccgaggct ggcggcgtcg
     1981 gaacaccgtc gtagcctgca atcggattga gggggatcag attgacgtgc gatggcaatc
     2041 catccagcaa ttgacccaag gcggcagcgt gctcggccga gtcgttttgt ccgtcgatga
     2101 gggcccactc gaagaagatc cgtcgttgga gcttgcctgc atagtatcgg caggcggcca
     2161 tcaattcatc caggggccag gcccgcgcgg gcggaacgag cttcgcccgc tcttcctgcg
     2221 tggcggcgtg gagcgaaatg gccagattca cccgctgtct ctcatccgcc agccgcacga
     2281 tgccggggat gacgccgacc gtgctcagcg tgatatgccg cggcgcgaga gcgagtccgg
     2341 tgggctcgca gagaatcgcc accgccttca tcaccgcatc atagttatgc agcggctccc
     2401 ccattcccat gaggacaaca tttcgcaggg ccaggcctgg ctggtgcaat agccgattcg
     2461 cgtgcaccac ctgctgcacg atttcccccg tcgtgagctg acgcaccagc cccatctggg
     2521 ccgtggcgca aaacacacat cccatcgcac aacccacctg cgagctaacg caggccgtcc
     2581 accttccccg gaattgcatc agcactgctt ccactcgctg cccatccgcc atgcccagca
     2641 aaagcttccg cgtgaagcca tcggaggata ctgcctcgct gcattgctcc aggtcgtccc
     2701 aggagaattc ttcggccagc cttcgccgca gcgatgctgg aagctcactc atttcctgcg
     2761 ccgagcccgc tccctcgcgg tagatgtact tcatcacccg cgcggcatgg atgggagcga
     2821 tgctccacga cgacaaga
//
LOCUS       DASUGB010000983         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1915522, whole genome shotgun sequence.
ACCESSION   DASUGB010000983 DASUGB010000000
VERSION     DASUGB010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915522"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFV87_18625"
     CDS             complement(<1..234)
                     /locus_tag="VFV87_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105843.1"
                     /translation="MPFDPKLIHPDQAPLFPDGEIDLPPDLAALGEQLRDDARHLAAC
                     YPADRDAANSLPAGKQRSRRWKRIAVMATSAAAT"
     gene            complement(224..787)
                     /locus_tag="VFV87_18630"
     CDS             complement(224..787)
                     /locus_tag="VFV87_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5105844.1"
                     /translation="MQNLNIDETTRQYFARIQRAALVLCGNPWDADDLAQETFLVLAR
                     QGGKFEGRSSIYTWLYGILLNLDRRERRRYGMRRRKLRILWDNQPAGKELSPGANAAA
                     EVAEWKKSLWARVAKLPDGQRQSLVLRFSEGLRYEEIAAVLECPLGTVKSRIFHGLAA
                     LRRVLEEEGEESQQLPAFPGEDLSHAV"
     gene            complement(1041..1682)
                     /locus_tag="VFV87_18635"
     CDS             complement(1041..1682)
                     /locus_tag="VFV87_18635"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEX5105845.1"
                     /translation="MLLRPIRSSSRPKPGVPSDDSSLWTWLVGCWQWPSAALFAAAFL
                     FALLPLIAGLDGAIGLVFLQLPIYMLHQWEEHTGDRFRQYANRVIGGGREVLTPTATF
                     WINSLGVWGVDLAALYLAWAVTPAAGLIAAYLALVNSLLHIGPALVRREYNPGLVTAA
                     ILFPLAGGWCVLTIGATCGPAPHLIALGGAIGIHLLIVAYVASRLRRLCRAAS"
     gene            1861..3366
                     /gene="glgA"
                     /locus_tag="VFV87_18640"
     CDS             1861..3366
                     /gene="glgA"
                     /locus_tag="VFV87_18640"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX5105846.1"
                     /translation="MNIVFASSEVAPFAKTGGLADVCGSLPVELQRLGHNVAVITPAY
                     RQVKNSGLPIEPLGVKFDIPIGNKIVRGRLLRGQLPGSDVPVFFVEQDEYFDRPELYR
                     AKGEDYKDNCERFVFFCRAALESIRLLHLTVDVVHCHDWQTSLIPAYLHIEYAHTLGY
                     ESVASLLTIHNLAYQGIFWHWDMLLTGLDWKYFNWHQMEFYGKLNLLKTGLAFADWLT
                     TVSPQYAKEIQNDPLGCGLDGVLRQRADSLTGIVNGVDYSIWDPALDPHIAQQYDAST
                     WQAGKAACKAALQRELGLPEYPRTPVVALIGRLADQKGWDLVLDVMRRWATDMDVQWA
                     ILGTGEPYYHEALGSLARDFPQRIAAHMEFSDELAHRIEAGADLFVMPSRYEPCGLNQ
                     LYSLKYGTVPVVRATGGLLDTVTDATPESLAAGTATGFQFEAYNASVLEATLRRACDT
                     CRHDAPVWQQIALAGMNQDWSWTASAHRYLEVYAHACRRRQEERERQKLGR"
     gene            3448..>3845
                     /locus_tag="VFV87_18645"
     CDS             3448..>3845
                     /locus_tag="VFV87_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105847.1"
                     /translation="MPEYRQDPLSRRWVIVGGDRADRPNEFVEAANRQDSLPCPFCAG
                     NEAETPEAVVTYPAKGTAGWLVRVVPNKYPAVSTDEALCPTCQPLTPQPAGDAVPGFG
                     RHEVIIESPRHVASLSELTAEEAEAVFAVYR"
BASE COUNT          715 a         1272 c         1200 g          658 t
ORIGIN      
        1 tgttgccgcg gcgctggtgg ccatcacggc gatccttttc caccgccgcg agcgttgctt
       61 acccgcaggc agtgagttcg ccgcatcgcg atcggccgga tagcaggccg ccaggtgccg
      121 cgcgtcgtcg cgcaactgct cgccgagtgc cgccaaatcc ggcggcaggt caatctcgcc
      181 atccgggaag agcggcgcct ggtcgggatg aatcaatttc ggatcaaacg gcatgggaga
      241 ggtcctcgcc tggaaaggcg ggaagttgct ggctttcttc gccttcttcc tccaggacac
      301 gccgcagcgc ggccagccca tggaagatgc gcgattttac ggtgcccagc gggcattcga
      361 gcactgcggc aatctcttcg tatcgcagac cttcgctgaa ccggagcacc agcgactgcc
      421 gctgaccgtc cggcaacttc gccacgcggg cccacaggct ttttttccat tcggccactt
      481 ccgccgcggc gtttgctccc ggcgagagtt ccttcccggc tggctggttg tcccacagga
      541 tccgcagctt gcggcgacgc atcccatagc ggcggcgttc gcggcggtcg agattcagca
      601 aaatgccata cagccacgtg taaatgctgc tgcggccttc aaacttgccc ccctggcgag
      661 ccagcacaag aaaggtctcc tgggccagat cgtccgcgtc ccaggggttg ccgcagagca
      721 cgagcgcagc ccgctgaatt cgggcaaagt attgccgcgt tgtttcgtcg atgttcaggt
      781 tctgcacaca gccaccccag ttcccgtcgc cccgattcta acgcgcgggc gtcagtcaaa
      841 gcgacgaatc gccggtgggc ctgactcgaa ctaccctttt gtgccctggc gaagctcaga
      901 agttcagacc agtcgttcat cgcactttgc tcgccgaccg cggaggcaca ggtctcctca
      961 acttccttct gcacaaagac ttacagcgca atcctgcgct tgcaattgtg taccgcgatc
     1021 cacgacgcgc gttctgggct tcaggaagcg gcccgacaca atcgcctcag ccgactggcg
     1081 acatacgcca caatcagcag gtggattcca atggcgcctc cgagagcgat caggtgcggc
     1141 gccgggccgc aggtcgcgcc gatcgtcagc acgcaccagc cgccggccag gggaaagaga
     1201 atcgccgcgg tgacaaggcc cgggttatat tcgcggcgaa ccagcgcggg cccgatgtgc
     1261 agcagcgagt tgaccagagc gaggtaggcg gcaatcaggc cggcggcggg agtgaccgcc
     1321 caggctagat acagggccgc tagatcgacg ccccacacac ccaggctgtt gatccagaac
     1381 gttgctgtcg gcgtgagcac ctcacggcca ccgccaatca cgcggttggc atactgccgg
     1441 aagcggtcgc cggtgtgctc ttcccactgg tggagcatgt aaatcggaag ctgcagaaac
     1501 accagcccga ttgcaccgtc caggcccgcg atcaggggca gcagggcgaa cagaaaagcc
     1561 gccgcaaaca gggctgccga gggccactgc cagcagccga cgagccaagt ccaaagggaa
     1621 ctgtcatctg acgggacgcc aggcttcgga cgggagctgg agcggattgg tcgtaggagc
     1681 atgatggggc tcgcgggggg aggaagttcc caacccgcaa atattcccgc gcgccgatgg
     1741 cgcggtaagc tataccggcc gcccggtgcc cctcgccgct ggccatcagg ccggccatac
     1801 gttataacct gcgccgccga cacaccttgt gacgacaccc ttcgccggga gtacaccgct
     1861 gtgaacatcg tcttcgccag cagtgaagtc gctccattcg ctaaaacggg ggggttggcg
     1921 gatgtttgcg gctctctccc cgtggaactt cagcggctgg ggcacaatgt cgctgtcatt
     1981 acgcccgctt atcgccaggt caagaactcc ggtctgccga tcgagccgct gggggtcaaa
     2041 ttcgacatcc cgatcggcaa caagatcgtc cggggccgcc tcctgcgcgg ccagttgccg
     2101 ggctcggacg tcccggtgtt cttcgtcgag caggacgagt atttcgaccg gccggagctc
     2161 tatcgggcca agggcgagga ttacaaggac aactgcgagc ggtttgtctt cttctgccgg
     2221 gcggcgctcg aatcgattcg cctgctgcac ctcacggtcg atgtcgtcca ttgccacgac
     2281 tggcagacct cgctgatccc ggcctacctg cacattgaat atgcccacac gctgggctac
     2341 gagagcgtgg cctcactgct cacgattcac aatctggcgt accagggcat cttttggcac
     2401 tgggacatgc tgctcacagg cctggactgg aagtacttca actggcacca gatggagttt
     2461 tacggcaagc tcaacctgct caagacgggc ttggccttcg ccgactggct gacgaccgtc
     2521 agtccgcaat acgccaagga aatccagaac gatccgctcg gctgcgggct ggatggcgtg
     2581 ctgcgacagc gggccgactc gctcacgggc atcgtcaacg gcgtcgatta cagcatttgg
     2641 gacccggccc tcgatccgca cattgcccag cagtacgacg cctccacctg gcaggccggc
     2701 aaagccgcct gcaaagccgc cctgcagcgc gaactgggcc tgccggaata tccccgtacg
     2761 ccagtggttg cgctgattgg ccggctggcc gatcagaagg gctgggacct ggtgctcgac
     2821 gtgatgcgcc gctgggcaac cgacatggac gtgcaatggg ccattctcgg caccggcgag
     2881 ccctactacc acgaggccct tggcagcctg gcccgcgatt ttccacagcg gattgccgca
     2941 cacatggagt tcagcgacga gttggctcac cgcatcgaag cgggggcgga tctctttgta
     3001 atgcccagcc ggtacgagcc ttgcgggctc aatcagctct acagcctgaa gtacggcacc
     3061 gtaccggtgg tgcgggccac gggcgggctg ctcgacacgg taaccgacgc cacacccgaa
     3121 tcgctcgctg ccggcacggc aaccggcttc cagttcgagg catataacgc gagtgtgctg
     3181 gaggcgacac ttcgccgcgc ctgcgacacc tgccggcacg acgcccccgt ctggcaacaa
     3241 atcgccctgg caggtatgaa ccaggattgg tcctggaccg ccagcgccca ccgctacctg
     3301 gaggtctatg cccatgcctg ccgacggcgg caggaagaac gcgagcggca gaagctggga
     3361 cgctaagccc gccgtagccg cactcgccag agtgcgggcc attccgacag cccgcgttct
     3421 ggcgaacgcg gctacacgcc atttctcatg cccgaatacc gccaagatcc gctctcgcga
     3481 cgctgggtga tcgttggcgg cgaccgtgcg gaccggccga acgaattcgt cgaggcggcc
     3541 aatcggcagg attcgctgcc gtgcccgttc tgcgcgggga atgaggcgga aacgcccgag
     3601 gcggtcgtca cctatccggc aaaaggcaca gcgggctggc tggtccgcgt tgtgccgaac
     3661 aagtaccccg ccgtatcgac ggacgaagcc ttgtgcccca cctgccagcc gctgacgccg
     3721 caaccggccg gcgacgccgt gcccggtttc ggccggcacg aagtgatcat cgaatcgccg
     3781 cggcacgtcg ccagcctgag cgagttgaca gccgaagaag cggaggctgt gttcgctgtc
     3841 tatcg
//
LOCUS       DASUGB010000984         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_250330, whole genome shotgun sequence.
ACCESSION   DASUGB010000984 DASUGB010000000
VERSION     DASUGB010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250330"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..752)
                     /locus_tag="VFV87_18650"
     CDS             complement(18..752)
                     /locus_tag="VFV87_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5105848.1"
                     /translation="MTVSSERRVASANHSNQPAPTMDVLVIEDDEVLGKALRRGLEDA
                     GHRCTWAKNGEKGLDLALAQKCDAIVLDLMLPDRPGLEVLREIRAQGIRTPVVILTAL
                     GSVEERVDGLNAGADDYIIKPFSFPELLARLSAVSRRSLHLNSQQLRVGPLSLDLSTR
                     RVTRDSTEIDLTPTEFSLLEFLMRNAGQVLTRKMLSEHLWDSNWEGVTNVIEVHITRL
                     RGKIDRDFNPPLLQTIRGRGYVLRVV"
     gene            1009..>2375
                     /locus_tag="VFV87_18655"
     CDS             1009..>2375
                     /locus_tag="VFV87_18655"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEX5105849.1"
                     /translation="MASTAARLRTDAAPQRAVEATELQEFIDHAAHYLPSQGPLTVFV
                     HHNTIHALEDRRFPLAVKLALELYGCQPYLSEDRYREEVARGRIRAEDLAAVLEEDLG
                     DEADELVGLLGTRFHLRLAMLQHSLHAAPSAELRWVIAETDALRKFRYDVPLEMSKRV
                     IDSTRRWIMRDFRNGGAREVADSYSAELRGIIAELFGEFERSTVEDWTEAAWETFYLQ
                     LLWRISLSGVQNARRVPHPTVEQDRHRDLLLEATGHDSDELVNDVLIRFTSAFLDQGF
                     AHWTLPDRDAGFYKSFLELYSQPGGVLPAWMHGVRQELDRLKEGNVTPLECIEESLEL
                     LGVGVEERESFLMATLLAMRGWAGMIWQMETNAEWTVHPAPKGSLTEFLAIRLLLDCY
                     AVAFMAREQFGYSGTLGELRESIGERRGSSGDDLQMQRAFLFFQLAQFLGLTPEQLYR
                     QPPKT"
BASE COUNT          439 a          753 c          709 g          474 t
ORIGIN      
        1 atctgcggaa gaatctttca gaccacccgc agcacgtaac cgcgaccgcg gatcgtctgc
       61 aagagcggcg gattgaaatc gcgatcgatc ttcccccgca gccgcgtgat gtgcacttcg
      121 atcacattgg tgacgccttc ccaattcgag tcccacagat gctcgctgag catcttccgg
      181 gtgagcacct ggccggcgtt gcgcatgaga aactccagca gactgaactc cgtgggcgtg
      241 agatcgatct cggtgctatc gcgcgtcacc cggcgggtcg aaagatcgag cgacaacggt
      301 ccgacgcgta gttgctgcga attcaagtgc agcgaccgcc gcgagaccgc cgacagccgc
      361 gcaagcagct cgggaaacga gaagggcttg atgatgtagt cgtccgcccc ggcgttcagg
      421 ccatcgacgc gctcttccac cgagcccagc gccgtgagaa tgacgaccgg cgtccgaatg
      481 ccttgagcgc gaatctcgcg cagcacctcc aggcccggtc gatccggcag catcaagtcg
      541 agcacgatgg cgtcgcactt ctgcgcgagg gccagatcca gacctttttc gccgtttttc
      601 gcccaggtgc atcgatgtcc ggcgtcttcc aatccgcgtc gcagggcctt tcccaacact
      661 tcgtcgtctt caattaccag cacatccatc gtcggggctg gttgattcga gtggtttgcc
      721 gaggcgacgc gccgttcgct gctgactgtc acggccatga cttcctatgg ttggttcgag
      781 tgcggggatt ggtacatgga gctccgcggc tggaaactct gccttctatt ctacgtggcg
      841 gagaatgtta aagaattgta atattgcctc ggtgttccct tgccgccgga aaaagtatac
      901 caaatccaga tcgcacatga ccgaagcgtg ccgcgaactt gcgacgtact gccgcgctca
      961 ttcgttacaa attcgtacgt cgccccaccc ccctctcgag ctgcccccgt ggcatccact
     1021 gcggcgcgat tgcgcaccga cgccgctcct cagcgagcgg tcgaggcgac cgaactacaa
     1081 gagttcatcg accacgcggc ccactacctg ccttcgcaag gtccgctgac ggtctttgtc
     1141 caccataaca cgatccacgc gctggaggat cgccgctttc cgctggcggt gaagctggcg
     1201 ctggagcttt acggctgcca gccctatcta tctgaagacc gctatcgcga ggaagtcgcc
     1261 cgcggccgca ttcgagccga agatctcgcc gccgtcctcg aggaagatct cggggacgag
     1321 gccgatgagc tggttggact cttaggcact cgattccacc tgcggctggc aatgctgcag
     1381 cattcgctgc acgcggcgcc gagcgccgag cttcgctggg taatcgcgga aacggacgcg
     1441 ctgcgcaagt ttcgttacga tgtgccgcta gaaatgagca agcgcgtgat cgacagcacg
     1501 cgtcgctgga tcatgcgcga cttccgcaac ggcggcgcaa gggaagtcgc cgactcttat
     1561 tccgcggagc tgcgcggaat cattgccgag ctgtttggcg agtttgaacg atcgacagtc
     1621 gaggattgga ccgaagcggc gtgggaaacg ttctatttgc aactcctgtg gcgcatctca
     1681 ttgagcggcg tgcagaacgc ccgccgggtt cctcacccga ccgtcgagca agatcgccat
     1741 cgcgatttgc tcctcgaagc gaccggccat gactccgacg agctggtgaa cgacgtcctg
     1801 atccgcttca cgtcggcatt cctcgatcag ggctttgccc actggacgtt gcccgaccgc
     1861 gacgcgggct tctataaatc gttcctggaa ctgtacagcc agcccggcgg cgttctgccg
     1921 gcatggatgc acggcgttcg ccaggagctt gaccggctca aggaaggcaa cgttacgcct
     1981 ctggaatgca tcgaagagtc gctcgagctt ctgggcgtcg gcgtcgagga gcgcgagtcc
     2041 ttcctcatgg ccacgttgct ggccatgcgc ggctgggccg gcatgatctg gcagatggaa
     2101 acaaacgccg aatggactgt tcatccggcg ccgaagggct cgctcaccga gtttcttgcc
     2161 atccggctgt tgctcgactg ctatgccgtc gccttcatgg cccgcgagca gtttggttat
     2221 agcggcacgc tcggcgagct gcgcgagtcc atcggcgagc ggcgcggttc ttcgggcgac
     2281 gacttgcaaa tgcagcgggc atttctcttt ttccaactgg cgcaattcct cgggctgacg
     2341 cctgagcagc tctaccggca accccccaag acctg
//
LOCUS       DASUGB010000985         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1778133, whole genome shotgun sequence.
ACCESSION   DASUGB010000985 DASUGB010000000
VERSION     DASUGB010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778133"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFV87_18660"
     CDS             complement(<1..341)
                     /locus_tag="VFV87_18660"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5105850.1"
                     /translation="MQSQINSLQKQVEQANAAAASAKKASSDDLDLKVKWRGAPEFSS
                     ADGNFKMKLRGRLHADWNAINQDESITGRPNVDAFEIRRARLGIEGVVWGDVKYIVEA
                     DFAQDAVSLKDA"
     gene            complement(739..1875)
                     /locus_tag="VFV87_18665"
     CDS             complement(739..1875)
                     /locus_tag="VFV87_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX5105851.1"
                     /translation="MDARVENEVNYTARGTLPSTDSAGLDKPVHVSVENVVRTFGTTP
                     ALHGVSLDIRPGELVALLGPSGSGKTTLLRILAGLDFPTSGRVLFDGSDALKLTVQER
                     NIGLVFQSYALFKHMNVMDNIGFGLRVRPSSRRPSRRAIKERALQLLDFVQLTGLDKR
                     YPAQLSGGQRQRVAFARALAIEPRVLLLDEPFGALDAKVRRDLRRWLREIHDKTGHTT
                     VFVTHDQEEALELADRVVVMSQGRIEQVGTPDEVYDKPNSPFVFSFIGESNTLPVKVD
                     GGQIWHEGHQLNLDAKNAATGEAQFYFRPHDVDVAHELPGGIPGVVSGLRRHGASRRV
                     EIAVGDQSDRIEVDVPANLEPRIGDRLAILPHHWQLFHDSHANA"
     gene            complement(1885..2778)
                     /gene="cysW"
                     /locus_tag="VFV87_18670"
     CDS             complement(1885..2778)
                     /gene="cysW"
                     /locus_tag="VFV87_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX5105852.1"
                     /translation="MSDLSLPLGLDFIGASDRARSVTTERRLVRIALIGIAVIFLTLF
                     LLLPLISVFVEAFRAGLRAYWDAIVEPDALAAIRLTLLVAAIAVPLNVVFGIAASWAI
                     AKFEFPGKSFLNTLIDLPFSVSPVISGLVFVLLFGLQGYFGAWLREHDIQIIFALPGI
                     VLATIFVTFPFVARELVPLMQEQGTQDEEAALSLGASGFQTFWRVTLPNVKWALLYGV
                     ILCNARAMGEFGAVSVVSGHIRGKTNTLPLHVEILYNEYNFAAAFAVASLLALLALVT
                     LVIKVTLEWRYADQIAGSNRH"
BASE COUNT          615 a          828 c          873 g          555 t
ORIGIN      
        1 gcatccttga gcgagaccgc gtcctgcgcg aagtcggcct cgacgatgta tttcacatcg
       61 ccccagacga ccccttcaat accgagacgc gcccgcctaa tctcgaaggc atcgacatta
      121 ggccggccgg tgatcgactc gtcctggttg atcgcgttcc agtcggcatg cagacggccg
      181 cggagtttca tcttgaaatt gccgtcagca ctggagaatt ccggcgcgcc acgccatttc
      241 accttcagat cgagatcgtc gctgctggcc tttttcgccg aggcagcggc tgcgttggcc
      301 tgttcgacct gtttttgcag gctgttgatc tgcgactgca aggcctggat ttgggcctgg
      361 atcgccgcat tgtcttgtgc gctcgccgga gcgctcagtg ccagaacggc aacgacgcta
      421 gatcccgcga aaagatatcg tgctttgtcc cttatcagtc ccccgaccat gacccctctc
      481 cattgttttg gttagtccct gcttttcaga caataagtcg ataaactcta cctagtttat
      541 gtagaatagc aatagcttta gatttcaaag ttttagcgtt ccttggaacg ccattctcag
      601 gccgataatg gggacgtaat gggagggtcg ctaacacgtt gggcgcgcgg atggagagcg
      661 cgtccatgcg atgttgccgg ctgaaagcgg caagatctac ggcgcggacc taatcgaaat
      721 cgagctattg cctgcgggtc aggcgttcgc gtgactatcg tggaaaagct gccaatgatg
      781 cggcaggatg gccagccgat ctccgatcct cggctcaaga ttagctggca catcgacctc
      841 gatccggtcg gactggtcgc cgaccgcaat ctccacgcgc cgcgaagcgc catgacggcg
      901 cagtcccgac accacgcccg gaatgccgcc cggcagttca tgggcgacgt cgacgtcatg
      961 cgggcggaaa taaaattgcg cctcaccggt ggcagcattc ttggcatcga gattgagttg
     1021 atggccctcg tgccagattt gacctccgtc gactttcacg ggcagcgtgt tggactctcc
     1081 gatgaaggaa aagacgaaag gtgagttggg tttgtcatag acctcatcgg gagtgccgac
     1141 ctgctcgatc cggccttgac tcatcaccac cacgcgatcg gccagctcga gcgcttcttc
     1201 ctgatcgtga gtgacgaaga cggtggtgtg tccggtcttg tcgtggattt cgcgcaacca
     1261 gcggcgcagg tcgcggcgca ccttcgcatc cagcgcgccg aaaggttcgt cgagcaacag
     1321 cacgcgcggc tcgatggcaa gcgcccgggc gaaggccacg cgttggcgct gaccacctga
     1381 aagttgcgcc gggtaacgct tgtccagacc ggttagctgt acgaaatcga gcaattgcag
     1441 cgcgcgttct ttaattgccc ggcggctcgg acgacgcgat gacggccgca cgcgcaagcc
     1501 aaagccgata ttgtccatca cgttcatgtg cttgaagaga gcgtagcttt ggaacaccag
     1561 gccgatattg cgttcctgca cggtgagttt cagggcatcc gagccatcga acaggacccg
     1621 ccccgatgtc gggaaatcga ggccggcaag aatcctgagc agtgtggtct tgcccgagcc
     1681 ggatggtccc agcagggcca cgagttcgcc gggacggatg tcgagcgaca ccccgtgcaa
     1741 agcaggtgtc gtgccgaagg tcctgacaac gttctcgacg gatacatgca cgggtttatc
     1801 caaaccggca ctatcagtgg atggcaacgt gcccctagcc gtgtagttca cttcattttc
     1861 cacccgggcg tccatggttt tctcctagtg acggtttgaa ccggcaattt gatcggcgta
     1921 gcgccactcc agcgtgactt tgatgacaag cgttacgaga gccagtagag ctaggaggga
     1981 agccacggcg aaggcggccg cgaagttata ttcgttgtag agaatctcga catgcagagg
     2041 cagagtgttg gtcttgcctc ttatatgtcc ggacaccact gacacggcgc cgaattcacc
     2101 catggcgcgc gcgttgcaga ggatcacgcc gtaaagcagc gcccatttca cattgggcag
     2161 cgtcacccgc cagaaagtct ggaatccgga agcgccgagc gacagcgccg cctcctcatc
     2221 ctgcgtcccc tgctcctgca tcagcggtac gagctcgcgc gccacgaagg gaaaggtgac
     2281 gaagatcgtg gcaagcacga tgccgggcag ggcgaagatg atctggatat cgtgctcgcg
     2341 cagccaggcg ccgaaataac cctggagacc gaacagcagc acgaagacca agccggaaat
     2401 caccggcgag accgagaagg gaaggtcgat gagcgtattg aggaagcttt tgcccgggaa
     2461 ttcgaatttg gcgatggccc aggacgcggc gatgccgaac acgacgttta acggtacggc
     2521 gatcgcggcg acgagaagcg taagcctgat tgccgccagc gcgtcgggct cgacgatggc
     2581 gtcccaatag gcccgcaacc cggcacggaa cgcttcgacg aataccgaga tcagtggtag
     2641 cagcaggaac aacgtcagga agatgacggc aatgccgatc aaggcgatcc gcaccaggcg
     2701 ccgttcggtg gtgacggacc gggcacgatc cgaggcgcca atgaaatcga gaccgagagg
     2761 aagagaaagg tcgctcattg agcaaatctc cgtctctgcc atgcctggat gaggttgatc
     2821 gcgagaatga cgaggaaggc aatgaccagc atcgccgtgg cgatcgccgt g
//
LOCUS       DASUGB010000986         4932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_109156, whole genome shotgun sequence.
ACCESSION   DASUGB010000986 DASUGB010000000
VERSION     DASUGB010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109156"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFV87_18675"
     CDS             complement(<1..859)
                     /locus_tag="VFV87_18675"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5105853.1"
                     /translation="MIRNWQLIACGAIFILVVGAWQAFSIQSQPPAGSRTLPGARPTI
                     EAAAYSTLQAAIDSLPSEGGVVRLPAGTFEISQPLLITQEDVLLEGSGTATHIRNMNA
                     DGQPAIVIQSPKIKEDPRAVLWRVQIANLRVTGNEKSGPGILARHINEVFLHGVTVSY
                     HGGDGIQLDNCYEDPRICDCLLTYNKANGLNLVGCHDIVVVGNQFEENLDALRCTDSF
                     NLCMTGNNLDDHLRDGVVIENTYGSVVSGNMIEECNGRAIVLNRDCYGITLSANVIAH
                     NGGGIHLVDA"
     gene            1132..2415
                     /locus_tag="VFV87_18680"
     CDS             1132..2415
                     /locus_tag="VFV87_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105854.1"
                     /translation="MLTIDLGKSARYCDGMSRRSMLQLGVLGFGAACGGLSLPQLLRA
                     EAAAGVGSSNKAIINIHLGGGPSHQDMFDLKPNAPREFRGEFNPIATNVPGIEICEHM
                     PLLAQMADKYAVIRSLVGMFDDHSNFHTQTGFGRDSLRNIGGRPSIGSVVSKLLGPTG
                     SGAPCYIAYNDGPEGFLGPVHKPYRPQGGDLKLVGGMDESRLSTRTSLLGSLDKIRRD
                     VDASHKMEALDSFTQRAVGVVTSGKVADALDLDKEDKSVRERYGRNDGDSFLRARRLI
                     EAGVRVVTFDWGGWDTHGDNFNHLRNQLPKLDRAMSALLQDLSDRGMDKDVTVVMWGE
                     FGRTPRVNGGAGRDHWSRVAMGFLAGGGMRLGQAIGTSTANAEEAKDRPVHLQEIFAT
                     LYHNLGIDVQRTQIIDPAGRPQYLVEIREPIAELI"
     gene            2501..3544
                     /locus_tag="VFV87_18685"
     CDS             2501..3544
                     /locus_tag="VFV87_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5105855.1"
                     /translation="MPLIVLRIVCVAVAMGIATLLIGLVRSAGGPGWLPWVAFLTCAV
                     VAASVIAADIFLPRKRIDVISAIYFGLAIGFFLTFVAMLALTPLLQLLDDQPIISDSH
                     LFIIRTAMQLLLGLVLCYTCISVLMQTKDDFRFIIPYVEFSKEVKGLKPYVLDTSVVI
                     DGRIADLVETNVLDNQLIMPRFVLSELQAIADSGDKLRRARGRRGLDILNRLRNNQHI
                     DLKIHDHELPEMSGQPVDMKLVLLAKHLEGKIVTGDYNLNKVARLHNVAVINLNDIAN
                     SLKPVFLPGETLDVRIVKPGEEMGQGVGYLDDGTMIVVEGGREHIGKEVKVNVTSVLQ
                     TSAGRMIFGKAIA"
     gene            complement(3560..4327)
                     /locus_tag="VFV87_18690"
     CDS             complement(3560..4327)
                     /locus_tag="VFV87_18690"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5105856.1"
                     /translation="MFRSCLALVFIMPSTLSAADADHRDRFEARTFDDRGFTLPYRLL
                     KPQDLGARQKYPLVLFLHGAGERGNDNVKQLVHGMNDFASDEIMAKHPAFVVAPQCPE
                     DRKWVEVDWTLDAHTLPESPSVSLEATIRLIESLQKEFTIDASRIYITGLSMGGYGTW
                     DALARWPELFAAAAPICGGGDPAVAARFKDVPIWVFQGDEDTAVKPKRSREMVEALKA
                     AGGQPKYTEYPGVAHDSWTQTYADPTLHEWLFSQRKP"
     gene            4426..>4932
                     /locus_tag="VFV87_18695"
     CDS             4426..>4932
                     /locus_tag="VFV87_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5105857.1"
                     /translation="MSARASLALTALLLVARIAAAAEPAPNTLTDEEKAAGWKLLFDG
                     KTTEGWRNYKKPEIGEGWKVEDGALVRAAGGAGDILTKDKYGAFELSLEFNISKGGNS
                     GLMFHVTEEGGAPWHTGPEIQIQDNQDGHDPQKAGWLYQLYQPEGDLDAARPAGEWNQ
                     IRILITPEK"
BASE COUNT          954 a         1539 c         1510 g          929 t
ORIGIN      
        1 gggcgtcaac caggtggatt ccgccgccgt tatgggcgat tacgttggcc gagagggtga
       61 tgccgtagca atcgcggttc aagacgatcg cccggccatt gcactcctcg atcatgttgc
      121 cgctgacgac cgaaccgtac gtgttctcaa tgacgacgcc atcgcgcaag tgatcgtcca
      181 ggttgttgcc ggtcatgcac aggttgaagc tgtcggtgca gcgcagcgcg tcgagattct
      241 cctcgaactg gttgccgacc acgacaatat cgtggcaccc gaccaggttc aggccgttgg
      301 ccttgttgta cgtgagcagg caatcgcaaa ttcgcgggtc ttcatagcag ttgtcgagtt
      361 ggatcccgtc gccgccgtga tagctcaccg tcacgccgtg caggaaaacc tcgttgatgt
      421 gacgggccag aattcccggg ccgctcttct cgttgcccgt cacgcgcaaa ttggcgattt
      481 gcacccgcca cagcacggcc cggggatctt ccttgatctt cggcgactga atcacaatcg
      541 ccggctggcc gtcagcgttc atgttcctga tgtgggtggc ggtgccactc ccttccagca
      601 gcacgtcttc ctgcgtgatc aagagcggct ggctgatctc aaaggtcccc gccggcaatc
      661 ggaccacgcc cccctcggat ggcagcgagt caattgccgc ttgcagggtc gaatacgcag
      721 ccgcttcgat cgtcggcctt gcgcccggca gagttctcga accggcaggc ggctgcgatt
      781 gaatcgagaa agcctgccag gcgccgacga caaggatgaa gatggcgccg cacgctatga
      841 gctgccagtt gcgaatcatg agcttgatct ccaagtcggc caaggaccat tttcgaaagg
      901 gcacgtcatc gcgcgatatt gccctgaggc atgtaattct gacacgcgga catgcgattc
      961 tcaatttagg cacgtttgca gtttgatata aaaatccatt gattggtcct ggcttaaagc
     1021 cctacaatgg gagccagctc gccgggtgta acgactcccg tgcggccggc catatcaaat
     1081 tggcccaata agagattgcg gcctccgctt tcacgtccaa ggaaatgcca catgttgacg
     1141 atcgacctcg gcaagtcggc ccggtattgc gatggaatgt cccgccggag catgctccaa
     1201 ctgggtgtgc tgggatttgg agccgcgtgt ggcgggctgt ctctgccgca gcttctgcgg
     1261 gcggaggcgg cagcgggcgt aggctcgtcg aacaaggcga ttatcaatat ccacctgggc
     1321 ggcgggccga gccaccagga catgttcgac ctgaagccga acgccccgcg cgagttccgc
     1381 ggcgagttca acccgatcgc gacgaacgtg ccgggcattg aaatctgcga gcacatgccg
     1441 ctgctcgctc agatggccga caaatacgcc gtcatccgct cgctggtcgg catgttcgac
     1501 gaccacagca atttccacac gcaaacgggc tttgggcgcg attcgctgcg gaatatcggc
     1561 ggccggccga gcatcggcag cgtcgtcagc aagctcctcg gccccactgg cagcggcgcg
     1621 ccgtgctaca tcgcctacaa cgacgggccc gaagggttcc tcggtccggt ccataagccc
     1681 taccgcccgc agggtggcga cctgaagctg gtcggcggca tggacgaatc gcggctcagc
     1741 acgcggacga gcctgctcgg ctcgcttgac aagatccgcc gcgacgtcga tgcgtcgcac
     1801 aagatggaag ccctcgattc cttcacgcag cgggcggtcg gcgtggtgac cagcggcaaa
     1861 gtggccgatg ccctcgacct cgacaaagaa gacaagtcgg tccgcgagcg ctacggccgg
     1921 aatgatggcg actcgttcct ccgcgctcgc cgcctgatcg aagccggcgt gcgcgtcgtc
     1981 acgttcgatt ggggcggctg ggacacgcac ggcgacaact tcaatcacct gcgaaatcaa
     2041 ctgcccaagc tcgatcgggc gatgagcgcg ttgctgcaag acctctccga tcgaggcatg
     2101 gacaaggacg tgacggtcgt aatgtggggc gagttcggcc gcacgccgcg cgtcaacggc
     2161 ggcgccggac gcgatcactg gtcgcgcgtc gcgatgggct ttctcgccgg cggcggcatg
     2221 cggctggggc aggcgatcgg cacttcgacg gccaatgccg aagaagccaa ggatcgcccg
     2281 gtccatctgc aggagatctt tgcgactctc tatcacaacc tgggaatcga tgtccagcgc
     2341 acgcagatca tcgaccccgc cggccggccg cagtacctgg tcgagattcg cgaaccgatc
     2401 gccgaactga tctgaaggag gcaattccgg ctcgggcggc atccgctata ctaatcccag
     2461 cgcggaagat cctcgtgtcc ttgcaatccg gccggtcctg atgccgctca tcgtcctgcg
     2521 aatcgtgtgc gtggcggttg ccatgggcat cgccacgctc ttgatcgggc tggtgcgctc
     2581 tgccggtggt ccgggctggc tgccgtgggt ggcgtttttg acctgcgcgg tcgtggcggc
     2641 cagcgtcatt gccgccgaca tctttctgcc ccgcaaacgc atcgacgtca tctcggcgat
     2701 ctacttcggc ctggcgatcg gcttttttct cacgttcgtc gccatgttgg ctctgacgcc
     2761 tctgttgcag ctcctggacg accagccgat catcagcgat tcgcacctgt tcatcatccg
     2821 cacggccatg cagctcctgc tggggctcgt cctctgctac acgtgcatca gcgtcctcat
     2881 gcagacgaag gacgatttcc gcttcatcat cccctacgtc gagttctcca aggaagtgaa
     2941 gggcctgaag ccctacgtgc tcgacacgag tgtcgtgatc gacggccgca tcgccgatct
     3001 cgttgagacg aacgtcctcg acaaccagct catcatgcct cgcttcgtcc tttccgagtt
     3061 gcaggcgatc gccgattcgg gcgataagct ccgccgcgcc cgcggccgac gtggcctgga
     3121 catcctcaac cgtctccgca acaaccagca catcgacctg aagattcacg accacgagct
     3181 gcccgaaatg tccggccagc cggttgatat gaagctcgtg ctgctcgcca agcacctgga
     3241 ggggaaaatc gtcacgggcg attacaacct caacaaagtg gcccggctgc acaatgtggc
     3301 agtaattaac ctgaacgaca tcgccaattc gctgaagccg gtctttttgc ctggcgaaac
     3361 gctcgacgtc cgcatcgtca agccgggcga ggaaatgggc cagggcgtcg ggtatctcga
     3421 cgacggcacg atgatcgtcg tcgaaggggg ccgcgagcac atcggcaagg aggtcaaggt
     3481 caacgtgacc agcgtcttgc agaccagcgc cgggcgaatg atttttggga aagccattgc
     3541 gtagcacagg ttgccgacgt tacggcttcc gctgcgagaa cagccactcg tgcagcgtgg
     3601 gatcggcata ggtttgcgtc cagctatcgt gggccacgcc cggatattcg gtgtactttg
     3661 gctgcccgcc ggcggccttc agggcttcga ccatttcgcg ggagcgcttg ggtttgacgg
     3721 cggtatcctc gtcgccctga aagacccaaa tgggaacatc cttgaagcgc gccgctacag
     3781 ccggatcgcc cccgccgcaa atcggcgccg ctgccgcgaa gagctccggc cagcgggcca
     3841 aagcatccca tgtgccgtaa ccgcccatcg agaggccggt gatgtagatc cggctggcgt
     3901 cgatcgtgaa ttccttctgc agcgattcaa tcagtcgaat cgttgcctcc aggctgacgc
     3961 tgggcgattc cgggagcgtg tgcgcgtcca gcgtccaatc gacctccacc catttgcggt
     4021 cttccgggca ttgcggagcg accacgaatg ccgggtgctt cgccatgatc tcgtcggagg
     4081 caaagtcatt catgccgtgg acgagctgct tgacgttgtc atttccgcgc tcgccggcgc
     4141 cgtgcaaaaa gagcaccagc ggatatttct gccgggcgcc caagtcctgg ggcttcagca
     4201 ggcgataggg gagcgtaaac ccgcgatcgt cgaacgttcg ggcctcaaag cggtcgcggt
     4261 gatccgcatc cgcggcggaa agggtggaag gcatgatgaa caccagggca agacaggaac
     4321 gaaacatggt caaactccaa ccgaggacgt gcagaccgcc tgccggcgtc gtaggattgt
     4381 aatctcccat gtttggaatt tccccggtat tttaaggaga atcgaatgtc cgcccgcgcg
     4441 tccctcgcat taactgcctt gttacttgtc gctcgcatcg cggccgccgc cgagccggcg
     4501 cccaataccc tcaccgatga agaaaaagcc gccggctgga agctgctgtt cgacggcaag
     4561 accaccgaag gctggcggaa ctacaaaaag cccgagatcg gtgaaggctg gaaggtggag
     4621 gacggggcgc tagtgcgggc cgctggcggc gcgggcgaca ttctcaccaa ggacaaatac
     4681 ggcgccttcg agctgtcgct ggaattcaac atttccaaag gcggcaacag cggcctcatg
     4741 tttcacgtca ccgaggaagg cggcgcaccc tggcacaccg gccccgaaat ccagatccag
     4801 gacaaccagg acgggcacga tccgcaaaag gcaggatggc tctaccagct ttaccagccg
     4861 gagggcgatc tcgacgccgc caggccggct ggggagtgga atcaaattcg cattctgatc
     4921 acgcccgaga aa
//
LOCUS       DASUGB010000987         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2055950, whole genome shotgun sequence.
ACCESSION   DASUGB010000987 DASUGB010000000
VERSION     DASUGB010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055950"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..>2617
                     /locus_tag="VFV87_18700"
     CDS             257..>2617
                     /locus_tag="VFV87_18700"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5105858.1"
                     /translation="MTGIAHMALLTLALAGQVQRAEAPVAQEAPTAEEIQAAVGKLGA
                     DDFRVRQAASDLLWRAGRAAEAALREALKSNDPEVRFRAAAVLDKVRFGILPDTPPDI
                     LLLIDQFRHGSSSTVKRQSLLELQAKGQWSTILALLRGEENEQRRRELATAVAGEASK
                     LVRPMIEKGELDQAIEVLELTAVHDPGVAQLTAALLLSGRLDDEMARLAGQLAEKPRE
                     EDWRRLAMYQRAKGDLAAAAAAAEKTNDKYLVVNLLAEGREWANVASLMEELQSQNDS
                     QLDHAAFAAAFYHLAGDQAGYDRVLDRLQTAAGMNPEMGAQTPGFPADAVQVAKSWFL
                     IEALLVTEHLDEALAILKKTHPHNAHVLLWRQHRHREALELAGVAEGKALDRAWFDAL
                     PAPPGESRSQVAVRMALAGQVARELRELGRLDDIATIVETLRGLAAAAGDQGQRWTQV
                     AALNWQLGRYEEAFHDASQALAAKAAPPTVFGTLLKQQGQLATIWYEMTTAQDPLMER
                     DKAVERAVWLAVPNPPKGKLPADWKEILNREADAAGKLDPQLKLQRLIVLADTARVRG
                     DRDLMRKLLTAAVAVTEPTEPDPFATPGSITAMQRAAGLKLADLSAEDGDWAAAEQQY
                     LAVAKAAPADPLPLYLQGLALTKSKHGEEGEKLMRLAELTALAPESRAALAAGLLERG
                     HKELAAAQFEIVHRTATPDSSLVASAAQQIGNLINGQEPRRAAERWQELHLHVLNANS
                     NFVEVEGYLTLPHVMHKVQAKAALAEGKTDELMAELARCEKVLPGDI"
BASE COUNT          482 a          829 c          940 g          366 t
ORIGIN      
        1 cgccgaaaac gagcctccgc tcgccagtgc cagaagcgtc accagcagca cgtgcagtac
       61 gatcaggccg gcgaagacca ggaacaaccg ccagaaccac tgtgcaaatc ccatgtccca
      121 ttctccgcac gacaaaccgg cgggcgcacg atacccgccc cgcggactgc gccaaagggg
      181 gcagtcgcga tttggccgtc gtgtgccgga tcggctatgc tgaaggcgag attcagggct
      241 cgcaaggaag gaacatatga ctggcatcgc gcacatggct ctgctgactt tggctctcgc
      301 aggccaggta cagcgggcag aagctcctgt tgcccaggag gcgccgacgg cggaggagat
      361 tcaggctgcg gtgggcaagc tgggggcgga cgactttcgc gtccgccagg cggccagcga
      421 cctcttgtgg cgggccgggc gggcggcgga ggctgcgctc cgcgaggcgc tcaagagcaa
      481 cgatccggag gtccgcttcc gggcggcggc cgtgctggac aaggtccgct tcggaattct
      541 acccgacacg ccgccggata ttttgctcct gatcgatcag ttccggcacg gcagctcgtc
      601 cacggttaag cgccagtcac tcttggagct gcaggccaaa ggccagtgga gcacgatcct
      661 ggccctgctt cgcggagaag agaacgagca gcggcggcgc gaactggcca ctgccgtcgc
      721 gggcgaagcg agcaagctcg tccgcccgat gatcgagaaa ggcgagctcg accaggcgat
      781 cgaggtgctg gagctgacgg ctgtccacga tccgggcgtc gcgcagctca cggcggcgct
      841 gctgctctcg gggcgattgg atgacgagat ggcgcgcctg gcggggcagc ttgccgaaaa
      901 gccgcgcgaa gaggattggc ggcggctggc gatgtatcaa cgggccaagg gggacctggc
      961 ggctgcggct gccgcggcgg aaaagacgaa cgacaagtat ctcgtcgtaa atctgcttgc
     1021 cgaggggcgc gagtgggcca acgtcgcatc gctgatggag gagctgcaga gccagaacga
     1081 ttcgcagctc gatcacgccg cctttgccgc cgcgttttac cacctggcgg gcgaccaggc
     1141 gggctacgac cgggtgctcg accgcttgca gaccgcggcg ggcatgaatc cggagatggg
     1201 ggcccagacg cccggattcc cagccgacgc ggtgcaggtg gccaaaagct ggttcctgat
     1261 cgaggcgctg ctggtgactg agcatctcga cgaggcactg gccatcctga agaagacgca
     1321 tccgcacaac gcccacgtgc tcttgtggcg gcagcaccgg catcgcgaag ccctggagct
     1381 ggcgggcgtc gccgaaggca aagcgctcga ccgggcgtgg ttcgatgcgc tgccagcgcc
     1441 gccgggcgaa tcacggtcgc aagttgccgt gcggatggca ctggcgggcc aggtggctcg
     1501 cgagctgcgc gagctgggcc ggctggacga catcgccacg atcgtggaga cgctccgcgg
     1561 gctggcggcc gcggctggcg accagggaca gcgctggacg caggtcgctg ccctcaattg
     1621 gcagcttggc cggtacgaag aggcgttcca cgatgcttcg caggccctcg cggccaaggc
     1681 cgcgccgccc acggtcttcg gcacgctact caagcagcag ggacagctcg cgacgatctg
     1741 gtacgagatg acgacggccc aggacccgct gatggagcgc gacaaagccg tggagcgcgc
     1801 ggtgtggctg gccgtgccca atccgccgaa aggcaagctc cccgccgatt ggaaggagat
     1861 cctcaaccgc gaagccgacg ccgccggcaa gctcgacccg cagctcaagc tgcagcggct
     1921 gatcgtgctg gccgatacgg cccgtgtgcg gggcgatcgc gacttgatgc ggaagctact
     1981 gaccgcggcg gtggcggtta ccgagccgac cgagcccgat ccgttcgcta ctccgggcag
     2041 catcaccgcc atgcagcggg ccgccgggct caagctcgcc gacttgtcgg ctgaagacgg
     2101 cgactgggcc gcggccgaac agcaatatct ggcggtggcg aaagcggcgc cggccgatcc
     2161 gctgccgttg tatctgcagg gcctggcgct gacgaagagc aagcacggcg aggaggggga
     2221 gaagctgatg cgcctggcgg agctgacggc tcttgcgccg gaatcgcggg cggcactagc
     2281 cgccggactg ctagaacgcg ggcacaagga gttggcggca gctcagttcg agatcgtgca
     2341 tcgcacggcg acgcccgatt cctcgctcgt ggcaagcgcc gcccagcaga tcggcaacct
     2401 catcaacggg caagagccgc ggcgggccgc ggaacgttgg caagagctgc acctgcacgt
     2461 gctcaacgcc aacagcaatt tcgtggaggt cgagggctac ctcacgctgc cgcacgtgat
     2521 gcacaaggtg caggcgaagg ccgcgctggc cgagggcaag acggatgagc tcatggccga
     2581 actggcccgc tgcgagaaag tgctgccggg cgatatt
//
LOCUS       DASUGB010000988         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_250438, whole genome shotgun sequence.
ACCESSION   DASUGB010000988 DASUGB010000000
VERSION     DASUGB010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250438"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VFV87_18705"
     CDS             complement(<1..1554)
                     /locus_tag="VFV87_18705"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HEX5105859.1"
                     /translation="MWAADPLDARLRAGEFGPALEAAATEKDPAVRDAALGRIAAAQA
                     AAGAARASIGTVAEMNSDLARAEALKGVAGRGGFFGAGGGVQADFDPLIDLIQTTIAP
                     ESWTDVGGPGAIREFEAGVIVDPSGLLKKLAPDTDRSLALARRSAADVLNSGDPRRSS
                     PLRKISLTRLERQVQLLHALGREPDESMAVLAGLQRIKYVFFYPESGDIVLAGPAGDW
                     RRDPEGRIVSAEKGRPVVQLDDLVVVLRNAMSDDPRFGCSIDPTREGLAAAQAVNDKW
                     SARSLRPGQRDKWLEELRAAMGQQSISIHGIDPRTRAARVIVEADYRMKLVGMGLEEG
                     TLGVTSYLDSIELAKGQAPPPMSVLRWWFTLNYDALRGTAERDAFELRGPGVRVLSED
                     ELLTERGERMHTGKSNELNARFAASFTKHFETLAAKYPVYADLRNIFDLALVAAVIRS
                     HDLPEQGNWHLTHFGPDGGYAAELGIAPAKVDSVINHRVINGKHVVAGVSGGVRVDAT
                     SLAARAAVQT"
     gene            complement(1788..2504)
                     /locus_tag="VFV87_18710"
     CDS             complement(1788..2504)
                     /locus_tag="VFV87_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5105860.1"
                     /translation="MNLSLMITCLGDCLRPAAGKAVVRILRRLGHVIDFPGDQTCCGQ
                     PMFNSGYADLARQQAKHTIGVFAGEQPVIVPSGSCAAMVKVEYPHLLEGDDVWHPRAL
                     DLARRTFEFSDFLVNQLGVLDLGAKYDGKVAFHYACHLRMLGQTSEVERLVRHVQGAT
                     YVPLARQDQCCGFGGSFAVRYPLVSGNMVDDKMRCILASGANCVVSTDAGCMMNIGGR
                     LHREGHAVELLHIAELLERR"
     gene            complement(2501..2803)
                     /locus_tag="VFV87_18715"
     CDS             complement(2501..2803)
                     /locus_tag="VFV87_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105861.1"
                     /translation="MKVIFIAAARQELQSAIVHYNAQRPTLGDECKEEVDAPIDRIEF
                     WPNAWSQAPPNARVCKTKRFPYGIVYLVDPEIIIIAKAHLHRCQGYWKKRLKDLGP"
     gene            complement(2800..3024)
                     /locus_tag="VFV87_18720"
     CDS             complement(2800..3024)
                     /locus_tag="VFV87_18720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX5105862.1"
                     /translation="MTAQAIAAAALALPPESRVTLAEVLLNSVKGDSVSLSSEWLAEI
                     EDRIAAYRRGELATYSREEVMQSLEADSGR"
     gene            complement(3097..>3357)
                     /locus_tag="VFV87_18725"
     CDS             complement(3097..>3357)
                     /locus_tag="VFV87_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105863.1"
                     /translation="LGEGLAVAIRLIDEGGDADVMDSQGSGEIMVRNFDLREAEAHLR
                     RNLGSDAFDKPQSLRAVLDGVRQRHKQEQKVYHWDFSESDIR"
BASE COUNT          574 a         1134 c         1070 g          579 t
ORIGIN      
        1 ggtctggacc gcggcgcggg cggccaggct cgtggcatcg actcgcacgc cgccgctcac
       61 ccccgcgaca acgtgcttgc cgttgatgac ccggtgattg atgaccgaat cgaccttggc
      121 gggggcgatg cccagctcgg cagcatagcc cccgtcgggg ccgaaatggg tcaggtgcca
      181 gttcccctgt tccggcaggt cgtggctgcg gatcaccgct gcgacgaggg ccaggtcgaa
      241 aatgttgcgc aaatcggcat agaccgggta tttggccgcc agcgtctcga agtgcttggt
      301 gaacgaggcg gcgaagcggg cattgagctc gttgctcttg ccggtgtgca tgcgctcgcc
      361 acgctcggtg agcagctcgt cctcgctgag cacgcgcacg cccgggccgc gaagctcgaa
      421 tgcgtcgcgc tcggcggtgc cgcggagggc gtcgtagttg agcgtgaacc accaccgcag
      481 cacactcatc ggcggcgggg cctggccctt ggccagctcg atcgagtcga ggtagctggt
      541 cacgcccagc gtcccctcct ccaggcccat gccgacgagc ttcatccggt aatcggcttc
      601 gacaatcacg cgggcggccc gcgtccgcgg atcgatgccg tggatcgaaa tcgattgctg
      661 gcccatcgcg gctcgcaact cttccagcca cttgtcgcgc tggccggggc ggagcgagcg
      721 ggccgaccac ttgtcgttca cggcttgggc ggcggccagc ccctcgcgcg tcgggtcgat
      781 cgagcagccg aagcgcgggt cgtcgctcat cgcgttgcgc agcacaacga ccaagtcgtc
      841 cagctgcacg accggtcggc ctttctcggc tgagacgatg cgcccttcgg ggtcgcgccg
      901 ccaatcgccc gccgggccgg ccagcacgat gtcgcccgac tcgggataga aaaagacgta
      961 cttgatccgc tgcaggccgg ccagcacggc catcgactcg tcgggctcgc gccccagggc
     1021 atggagcaat tgcacctggc gctccaggcg ggtgagcgag atttttcgca gcggcgagct
     1081 gcggcgcggg tcgccgctgt tgagcacgtc ggccgcgctg cggcgggcca gtgcgagcga
     1141 acggtcggtg tcgggcgcga gcttcttcag aaggccgctg ggatcgacga tcacgccggc
     1201 ctcgaattcg cggatggcgc cggggccgcc gacgtcggtc caggattcgg gcgcgatcgt
     1261 ggtctggatc aggtcaatga gcgggtcgaa gtcggcttgc acgcccccac ccgcgccgaa
     1321 gaagccgccg cggccggcga cccccttgag ggcctcggcc cgagccaggt cgctgttcat
     1381 ttcggccacg gtgccgatcg aagcgcgggc agcgcccgcg gcggcctggg cggcggcgat
     1441 ccggcccagc gctgcgtcac ggacggctgg gtctttctcg gtagccgccg cctccaaggc
     1501 ggggccaaat tcgcccgccc gcaagcgcgc atccagggga tcggccgccc acacgacgga
     1561 ggttgacaat agcggcccga ccaaggtcgc aacaaggaga acaaggttcg gtgcgacgca
     1621 ctcaatcatg cgcgccgcgc aaaggcgaag cgttcgccgg gacataaacc acctcttctc
     1681 actgcccctg agaagatctt cctgtggctt cgagtgtcgc acatcgcggc gggccgcgtc
     1741 aagcaacaaa tccaaaacgc cgatttgcac tgactcttcg ccgccaatta ccgccgctcg
     1801 agcagctcgg caatgtgcaa tagctcgacg gcgtgccctt cgcggtgcaa gcggccgccg
     1861 atgttcatca tgcaccccgc gtcggtcgaa acgacgcaat tggccccgct cgccaaaatg
     1921 caccgcatct tgtcatcgac catgttgccg gaaaccagcg ggtagcgcac ggcgaacgac
     1981 ccgccgaacc cgcagcactg gtcctggcgg gccagcggca cgtaggtcgc cccctggacg
     2041 tgccgcacca gccgctcgac ttcgctcgtc tggccgagca tccgcaggtg gcaggcgtag
     2101 tgaaaggcga ccttgccgtc gtatttggcc cccaaatcga ggacgccgag ctgattcact
     2161 aaaaaatcgc tgaattcaaa cgtccgccga gccaggtcca gcgcccgcgg atgccaaacg
     2221 tcgtcccctt ccaagaggtg tgggtattcc accttgacca ttgccgcgca cgagccgctg
     2281 ggaacgatca cgggctgctc gcccgcgaat acgccaatcg tatgtttcgc ttgctggcgg
     2341 gccaggtccg cgtagccgct gttgaacatg ggctgtccgc agcacgtctg gtccccggga
     2401 aagtcgatca cgtgcccgag ccgccgcagg atgcgcacca ccgccttgcc ggcggcgggc
     2461 cgcaggcagt cgcccaggca ggtgatcatg agggagaggt tcacggaccc aaatccttga
     2521 gccgcttttt ccaatacccc tggcaccgat gcagatgcgc tttggcaata atgatgatct
     2581 ctggatcgac gagatagacg attccatatg gaaatcgctt ggtcttgcag acccgagcgt
     2641 tgggcggcgc ttgtgaccag gcgtttggcc agaattcaat gcggtcgatc ggcgcatcca
     2701 cctcctcctt gcattcatcg cccaaggtgg ggcgctgcgc attgtagtgt acgatcgcgc
     2761 tctggagctc ttggcgcgcc gcggcgataa agatgacctt catcgcccgc tgtctgcctc
     2821 aagcgactgc atgacttctt cacgcgaata cgtcgccagc tcgccgcggc ggtatgcggc
     2881 gatgcgatct tcgatttccg ccagccattc gctcgaaagg gaaacggaat cccctttgac
     2941 actgttgaga agaacctcgg cgagcgtaac acgcgactcg ggaggcaagg cgagggccgc
     3001 agcagcaatc gcttgggcag tcatggcgta actcctttga ttaacctctc aattatacac
     3061 cggcgtgggc cgtccagccg atcgcccgga aactcttcag cgaatgtcgc tctcgctgaa
     3121 gtcccagtgg tacaccttct gctcctgttt gtgccgctgc cgcacaccgt ccagcacggc
     3181 ccggagcgac tgcggcttgt cgaaggcgtc gctgcccagg ttgcggcgca agtgagcttc
     3241 tgcctcgcgc agatcgaagt tccgcaccat gatctcgccc gagccctgcg agtccatcac
     3301 gtccgcgtct cccccttcat cgatcagccg aatcgccacc gccagccctt cgcccag
//
LOCUS       DASUGB010000989         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1221712, whole genome shotgun sequence.
ACCESSION   DASUGB010000989 DASUGB010000000
VERSION     DASUGB010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221712"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..722)
                     /locus_tag="VFV87_18730"
     CDS             complement(168..722)
                     /locus_tag="VFV87_18730"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5105864.1"
                     /translation="MSIAASPLLTADAFARMPDPGHPQELVRGAIIDMPPPKPRHGQV
                     CVNVCFYLRSFVEAHRLGHVVANDTGVITGQDPDTVRGMDVAFISFTKFPPGPLPASY
                     FTVAPDAVFEVRSPGDRRSEIYRKICEYHDIGVPAVYVLDPETSRVHCYFPDRPEEIL
                     NVTDQFAGIGPLADFSVPVAKFFE"
     gene            983..1621
                     /locus_tag="VFV87_18735"
     CDS             983..1621
                     /locus_tag="VFV87_18735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105865.1"
                     /translation="MDSLPRTFAAHQAGALPLAAGEVRPAVAVEHEGHRGLVAAVQRG
                     DHRAFTALIELYQNTVYGFLRARLIEPADAEDLCQEVFLRCYLGREKLSRAAAVGPWL
                     IGIARNLLREHVRRVQRRKEVAWTELCLELDALASEHDERPDDALAHLPGCLDSLGQS
                     AREAIDLRYRHQLRMVQIGERLRRSEGAVKLLVHRARAALRHCLETKFKNPR"
     gene            1641..>2555
                     /locus_tag="VFV87_18740"
     CDS             1641..>2555
                     /locus_tag="VFV87_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105866.1"
                     /translation="MTDQQLVELLEHKTPEELTPEEVELLRSRLDESAELRERLMNQL
                     QMETYLATALARVEITPEKIVARAQQQTPASSGGVFLVLVILICLPLLVLGAAALRYA
                     VRQQGTSEVATTAHAAPAGPANIDPTGKEKSATQNEAPAKNEATAGTHGADPARETSG
                     KMPPPAAAGDRSPRTPPKPPSPADVQTTEGTKPPAAKSPPAPPPAPWQPALDQSGEPP
                     PFAEIAFQPLDTAKSIPQREDLSAWFEKGPDSNLQLHRVDTRYGPCGAIEGVARLKSP
                     WPQDSALRLALENYKDLMIHCYHGQQGVT"
BASE COUNT          471 a          805 c          867 g          412 t
ORIGIN      
        1 tagtgcgaat ggcagtgcag gtggacgaag gggcgggttt ctgcggcggc ggtcatgcgg
       61 caatccttgc aagcgagcgt agcgggcaag cagcgtcgtg ccgccccgag aaaaatctgg
      121 ccaagggaga ggccgattat atcgcggccc agagatcgcc gaggccgcta ctcaaagaac
      181 ttcgccacgg gcacagaaaa atcggccagc ggcccaatgc ccgcgaactg atcggtgacg
      241 ttgaggatct cttccggccg gtcagggaaa tagcaatgca cgcggctggt ttccggatca
      301 aggacgtaca cggcaggtac gccgatatcg tgatactcac agatcttgcg atagatctcg
      361 ctgcgtcgat cgcctggaga acggacttcg aacactgcgt ccggtgcgac cgtgaaatag
      421 ctggcaggca gcggaccagg aggaaacttg gtgaagctga taaacgccac atccatgccg
      481 cgaacggtat ctggatcctg gccggtaatg acgccggtgt cgttggcaac aacgtgtccc
      541 agacggtgcg cctcgacgaa cgaacgcaga tagaaacaga cgttgacgca aacctgacca
      601 tggcgtggtt tcgggggtgg catatcaatg atcgctccgc gcacgagctc ttgcgggtgg
      661 ccggggtcgg gcatccgcgc gaaggcgtcg gccgtcagca ggggtgaagc ggcaatcgac
      721 atcgagcacc tggtgcgttt gcggggagcg tcgcattgac gccctggtat cctgattctg
      781 aattttacca caagcgccaa cggcggccaa attggctctg ccgcgcccgc gcgtgtaacg
      841 attgccctga acggggacca ctgctgtttg cctgcgcttc ggccggcggg ctatgatgga
      901 ggctctcgcc cgtggccagc cgtcgtctcc ccaccggtct gacttccaag ctctattccg
      961 cccgcctgcc ctggcccatc gcatggattc gctcccccgt acttttgccg cccaccaagc
     1021 cggcgcgctg ccgttggcgg cgggggaggt tcgcccggcc gtagcggtcg agcacgaggg
     1081 gcatcgcggt ctggtggcgg cggtgcagcg cggggatcac cgggcgttta ccgcgctgat
     1141 cgagctgtat cagaacacgg tctatggctt tctgcgggcg cgcctcatcg agccggccga
     1201 tgccgaggac ctttgccagg aggtcttcct tcgctgctac ctggggcgcg aaaagctcag
     1261 ccgggcggcc gcggtggggc cgtggctgat cggcatcgcc cgcaatctgc tgcgcgagca
     1321 tgtgcggcga gtccagcggc gcaaggaggt ggcctggacc gagctgtgcc tggagctgga
     1381 cgccttggcg agcgagcacg atgagcggcc cgatgacgcg ctggcccact tgccgggctg
     1441 cctcgattcg ctggggcaat cggcccgcga agcgatcgac ctgcggtacc gccaccagct
     1501 gcggatggtc cagatcggcg agcggctgcg gcgcagcgag ggggccgtga agctgctggt
     1561 acaccgggcg cgggcggctt tgcggcactg cctggaaacg aaattcaaga acccgcgctg
     1621 agacgccagg cggcctgacg atgaccgacc aacagctcgt cgaattgctc gagcacaaga
     1681 cgccggagga gctgactccg gaggaggtcg agttgttgcg ctcgcggctg gacgagtcgg
     1741 ccgagctgcg cgagcgattg atgaaccagc tgcagatgga gacgtacctg gcgaccgcct
     1801 tggcccgggt cgagattacg ccggaaaaaa tcgttgcccg cgcccagcag caaacgccgg
     1861 ccagttccgg cggcgtattt ctggtgctcg tgatcctcat ttgcttgcca ttgttggtgc
     1921 tcggggccgc ggcgctgcgg tatgcggtgc ggcagcaggg cacgtccgag gtcgcaacga
     1981 ctgcgcacgc ggcacccgct ggtcctgcga atatcgaccc cacgggcaaa gagaagtcag
     2041 caacacaaaa cgaggcgcca gcgaaaaacg aggcgacagc cggcactcac ggcgctgatc
     2101 ctgcgcggga gacttccggg aagatgccac cgccggcggc ggcaggcgat cgctcacctc
     2161 ggacgcctcc aaagccgcca tccccggccg acgtccaaac gacggagggg accaagcccc
     2221 ccgcggccaa gtcgcccccc gctccgcctc ctgctccttg gcagccggct ttggatcaat
     2281 cgggcgagcc gccgccgttc gccgagatag ccttccagcc gctcgacacg gcgaagtcga
     2341 taccgcagcg cgaggacctg agcgcctggt tcgagaaagg cccggacagc aatctgcagc
     2401 tgcaccgggt tgatacgcgc tacggccctt gcggcgcgat cgagggagtg gcgcggctca
     2461 aatctccctg gccgcaagat tcggcacttc gcctggccct ggagaactac aaggacctga
     2521 tgatccactg ctaccacggc cagcaggggg tgacc
//
LOCUS       DASUGB010000990         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_665656, whole genome shotgun sequence.
ACCESSION   DASUGB010000990 DASUGB010000000
VERSION     DASUGB010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665656"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..1054)
                     /locus_tag="VFV87_18745"
     CDS             complement(383..1054)
                     /locus_tag="VFV87_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5105867.1"
                     /translation="MDLGPSAARPRAVVFDLDGLMFNTEELYVEVGTELLRRRGFEFS
                     HELLDKMMGRPSHVALQIMIDTHTLQATVEELLAETDELFPEILRQRLAPMPGLIDLL
                     AALERHHIPKGIATSSRRSFVERVLGKFSFQPRFSPILTSEDITHGKPDPEIYLKAAA
                     ALGIAPGEMLVLEDSQNGCRAAIAAGAIAVAVPSGHSHRHDFTGAALVADSLADRRIY
                     KLLGL"
     gene            1270..>2520
                     /locus_tag="VFV87_18750"
     CDS             1270..>2520
                     /locus_tag="VFV87_18750"
                     /inference="COORDINATES: protein motif:HMM:NF023990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HEX5105868.1"
                     /translation="MTSTEQRRGDSPPADTTAREHFIPLRPAELVQKLADDPALTIPE
                     RQQFRQFCQLVEATIHHEYWTRLQALKAAYAPFDPDSDAYGNAATEGVSCTAAGGPPS
                     AQALFEQFDELLMRANYRRLPREELERAVEEPNQTGLHLHLNLDIFERLEIYVRGNAT
                     TTKTCRTWRSLWQPRSVSMPVHRRLALIFRLQQVTPATDRLDTRAVVLKLFKDIPHQD
                     VETLLPGACIRIGLIEQAKIVLPTLSGVGLTIIKLLKGAAAVAFVSLYGLIAFLALIS
                     GAIGYGVRSFFGYLRTREKHQLSLTRHLYFQNLDNNSGVIYHLLNEAEEQEFREMVLA
                     YWLLWRGRQSGATTEQLDTAAETWLRERCSLEVDFEISDALAKLQRLGLAQSTLTRSV
                     SKGVGSRWQAAPIESALETLDRAWD"
BASE COUNT          474 a          757 c          840 g          449 t
ORIGIN      
        1 ggtgttgcgc ggtgcaaacc attggaacaa cgggagttag gagaatgagg cgcccgcgag
       61 gggcggcgaa aaaaaaattg ggtaagttgc gggccgcgcc cggcggaaag cgaaggatcg
      121 gttcgcaggc gaatcgaggc cggtgggcgg tccaaagctc attaacggct gcaagtcgaa
      181 accgcgcaac agcgcaacat cctcatgcgg ggggtgttgc gcggcgcaag ccaatggaat
      241 gacaggagtt gaggcaatga attgcgggct gtggcggcgt gaaaaaactg aaaaaattgc
      301 gcgcagcgcc ctgggttgcg cgcggccgga gcttcgggaa cattccgtcc gcggtagccg
      361 gttttcccgc acattgcgga gactacaggc ccagcagctt gtaaatccgc cggtcggcga
      421 gcgaatcggc cacgagtgca gcgccggtga agtcgtggcg gtggctgtgg ccgctgggaa
      481 cggcgacggc gatcgcgccg gcggcgattg cggcccggca gccgttctgg ctgtcttcca
      541 ggacgagcat ctcgccggga gcgatgccga gcgccgcggc ggctttgagg tagatctcgg
      601 ggtcgggttt gccgtgcgtg atgtcttcgg atgtcaggat cggcgaaaac cgcggctgga
      661 agctgaattt gcccaggacg cgctcgacga acgaccgccg gctgctggtg gcgatgccct
      721 tggggatgtg gtgccgctcc agtgccgcga ggaggtcgat cagaccgggc atgggtgcga
      781 gccgctggcg gaggatttcc ggaaacagct cgtcggtctc ggccagcagc tcctcgacgg
      841 ttgcctggag cgtgtgcgtg tcgatcatga tctgcagggc cacgtggctc ggccggccca
      901 tcattttgtc gagcagttcg tgcgagaact caaagccgcg gcggcgaagc agctccgtgc
      961 cgacctccac gtacagctct tcggtgttga acatcaggcc gtcgaggtcg aagacgacgg
     1021 cgcgggggcg ggcggcagat ggtccaaggt ccaaggtcca aagtccaaag taggggtggg
     1081 tgtcgggatc agtggtcgga gatcggaatt cagcggtcaa acggccgtac cgaccgagta
     1141 ctcaacccat tcaattctgc cgcgaattgt gattctgcgc aagggcggca aggcagcgag
     1201 cctgctggcc gtcggcggat cgattgctag ctggagtgct tctcgaagaa aaaagccgat
     1261 gaacttaaga tgaccagcac cgagcagcgc cgcggggatt cgccaccagc cgacaccacc
     1321 gcgcgcgagc atttcattcc gctgcggccg gcggagctgg tgcagaagct ggcggacgat
     1381 ccggccctca cgattcctga gcgccagcaa ttccggcagt tttgccagct tgtcgaagcg
     1441 acgatccatc acgaatactg gacgcggctt caggcgctga aagctgctta tgcgcctttc
     1501 gatccggata gcgacgcgta tgggaacgcg gccacagagg gtgtttcctg caccgcggcg
     1561 ggcggtcccc ccagcgccca ggcgctcttc gagcagttcg atgagctgct tatgcgggcc
     1621 aactaccgcc ggctgccgcg cgaggagctg gagcgggccg tcgaggagcc gaatcagacg
     1681 gggcttcacc tgcatctgaa tctcgacatc ttcgagcggc tggagatcta tgtccgcggc
     1741 aacgccacaa cgacgaaaac ctgtcgtacc tggcgatcgc tttggcagcc gcgatcggtg
     1801 tcgatgccgg tccatcgccg cctggcgctc atctttcgtc tgcagcaggt cacgccggca
     1861 accgatcgcc tcgatacgcg ggccgttgtg ctcaagctct tcaaggatat tccgcaccag
     1921 gacgtcgaaa ccttgctccc gggtgcgtgc atccgcattg gcttgatcga gcaggccaag
     1981 atcgttctgc ccacgctctc cggcgtcggc ctgacgatca tcaagttact gaagggggcc
     2041 gccgccgtcg cgttcgtcag tctgtacggc ctgatcgcct tcctggccct gatcagcggc
     2101 gcgatcggct acggcgtccg ctcgtttttc ggctatttgc ggacccgcga aaagcatcag
     2161 ctcagcttga ctcggcatct ctattttcag aacctcgaca acaattccgg tgtgatctat
     2221 cacctcctga acgaggccga agagcaggag tttcgcgaga tggtgctggc gtactggctg
     2281 ctctggcgcg gccgccaatc cggcgctacc accgagcaac tcgacaccgc agccgaaacc
     2341 tggctccgcg aacgctgctc cctggaagtc gatttcgaga tcagcgacgc cctcgcgaag
     2401 ttgcagcggc tgggcctcgc tcaatccaca ctaacccgaa gcgtcagcaa gggagtcggc
     2461 agccgctggc aggccgcgcc gatcgaatcc gccctcgaaa cgctcgaccg cgcctgggat
//
LOCUS       DASUGB010000991         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_665738, whole genome shotgun sequence.
ACCESSION   DASUGB010000991 DASUGB010000000
VERSION     DASUGB010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665738"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3732)
                     /locus_tag="VFV87_18755"
     CDS             complement(<1..>3732)
                     /locus_tag="VFV87_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5105869.1"
                     /translation="AAPRLPALGVAVPRALDEVIQRLLRKDPRDRYQSAEAVLADLNA
                     IAAAVARGEDDPAIVVGAHDTRQSLSEPAFVGRREELHELERQVEQAQEGRGGLTLLE
                     GESGGGKTRLLTEAANRALGRGGWFLWGQGTSDVAPQPFSLLAGVVQGFLAAARSQPG
                     LVASIRERMGDQLSALTGALPGLAEILGQSSSYSTAPEAAGEMRTLSALHRFLSALGT
                     PARPLLLVLDDCQWADELTYRLLRRWLAPAESETANRHVHVIVAFRSEEVAEGHPLRR
                     LRTESHLRLAGMTADELRQLLESMAGRLPDDVVLAIVRCAENSPFMASAVLRGLVETG
                     KLVQDSGGWRSEALDLDEMQSSSRAAEILARRLELLPAETQKLLATGAVLGKEFDLDM
                     AVSLAELDPATALRQLDVARRRQLVWLRSGGAQCVFVHDKIRETLLARLPQAERQSLH
                     ARAAQYLQSRDDEFAQVAFHFDAAGQPHAALPYALQAAKTARARYALEAAQQQYEICE
                     RGAFWADATTRYHIALGLGEVLLLRGRYDEAGKKYNAAVDYAEGTLAQAEIRGKLGEL
                     AFKRGDMEQAIACYEHGLRSLGKYVPVRWPILLTLVAWQGFKQVLHTLLSCWLLHRLG
                     RLPNEEERLTLRLLSNLAHGYWYCRSLVHVLWAHLSGMNLAERFLPTPELAQCYAEHA
                     PGLTLVGYLSRAERYAQKSLAIRRELGDWWGQGQSLHYWGVVLYTGGRFQACIEKCRE
                     GIRLLHRTGDYWQVHIARYQIAASWYRLGELARAVEESQQNYESGIELGDEQASGIIL
                     DVWVRAALGEVPPRIFQEELQRPRHDAQGQAQVLFAEGVRQLFAENWHQAEQFLAEAI
                     ETADRAGVRNAYTLPYRPWLATALRRQAAELHDLMPDRRAALLRRAWTVARRARNGVW
                     LCRNDLPHVLREQGLIAAMQGKLQQARRYLDRSLAIAHKQHARYELAQTLEARAQLAR
                     EAGWKGADKDHFEAQAILASLLAARRQHQAQDDAPPTLSLADRFDGVLDWGRRIASAL
                     SAEVIYDVARIAALRLLRAEHCVVLSVDQSEGQPQFSRLVGEIPGEWDEDHVNESIAA
                     RHAIGFLEDVPASQRGAEDTKRSALCVPLYVRGELAACLYVTHEHVRGLFGPVEERLA
                     DYIVTIAGAALENAAGFSQLQSLNETLEIRVADRTAAAESRARELATSNQQLEQLTGE
                     LLLTQDELKGAKQIAESASEAKSRFLATMSHEIRTPLNGV"
BASE COUNT          584 a         1277 c         1253 g          618 t
ORIGIN      
        1 acgccgttga gcggcgtgcg gatctcatgg ctcatcgtcg ccaggaagcg gctcttggct
       61 tcgctggccg attcggcgat ctgcttggcc cccttgagct cgtcctgcgt cagcagcaat
      121 tcgccggtaa gctgctcgag ctgctggttg gaagtcgcca gttcgcgggc tcgcgactcg
      181 gctgccgcgg tgcgatcggc cacgcggatt tccagcgttt cgttcaggga ctggagctgc
      241 gagaacccgg ctgcattctc cagcgccgcg ccggcaatgg tgacgatgta gtcagccagc
      301 cgctcctcca cgggtccgaa cagtccccgg acgtgctcgt gcgtgacgta caggcaggcg
      361 gccagttctc cgcgaacgta caacggcacg cagagcgccg agcgcttcgt gtcttcagcg
      421 ccacgttgac tggctggaac atcttcgaga aatccgatgg cgtgcctggc cgcaatcgac
      481 tcgttcacgt ggtcttcatc ccattcacca ggaatctctc cgaccaggcg cgagaactgc
      541 ggctgtccct cgctttgatc gacgctcagg acgacgcaat gttcagcccg cagcaggcgc
      601 agggcggcga tgcgggcgac gtcgtaaatc acttcagccg acagggccga ggcgatgcgg
      661 cggccccagt cgaggacgcc gtcgaagcgg tccgccaggg agagagtcgg cggcgcatcg
      721 tcctgggcct ggtgctgccg gcgagcggcc agcagggaag ccaggatggc ctgagcctcg
      781 aagtggtcct tgtcggcccc cttccagccg gcttcgcggg cgagctgcgc gcgggcttcc
      841 aaggtctggg ccaactcgta gcgggcgtgc tgtttgtggg cgatcgccag gctgcgatcc
      901 agatagcgcc gcgcctgctg cagcttgccc tgcatggccg cgatcagtcc ctgttcgcgc
      961 aggacgtgcg gcagatcgtt gcggcacagc caaacgccat tgcgggcgcg gcgggcaacc
     1021 gtccaggccc gccgcagtag cgccgcgcgg cggtccggca tcaggtcgtg cagctctgcc
     1081 gcttgtcggc gaagcgccgt cgccagccac ggccgatacg gaagcgtata ggcattgcga
     1141 acgccggccc gatcggcagt ctcgatcgcc tccgcgagaa actgctcggc ctgatgccag
     1201 ttttcggcga acagttggcg gactccttcc gcaaacagca cttgggcctg cccctgcgca
     1261 tcgtgccgcg gccgctgcag ctcctcctgg aagatgcgcg gcggcacttc gcccagcgcc
     1321 gcccgcaccc agacgtccag aatgatgccc gaggcctgtt cgtcacccag ctcgatgccc
     1381 gattcgtaat tctgctgtga ttcctccacc gcgcgggcca actcgccgag ccgataccac
     1441 gaagcggcga tctggtagcg ggcgatgtgt acctgccaat agtcgcccgt gcggtgcaac
     1501 aggcggattc cctcgcggca cttttcgatg caggcctgga agcggccgcc ggtatagagg
     1561 acgacgcccc agtagtgcag cgattgcccc tgcccccacc agtcgcccaa ttcgcggcgg
     1621 atggcgagcg acttctgggc gtagcgctcc gcccggctga ggtagccgac gagcgtgagg
     1681 cccggcgcat gttcggcata gcactgggcc agctcggggg tcggcaggaa tcgttcggcg
     1741 aggttcattc cgctcagatg ggcccacagc acgtggacca ggctgcggca ataccagtag
     1801 ccgtgggcca ggttgctgag cagccgcagc gtcagccgct cttcctcatt gggcaaacgc
     1861 cccagccggt gcagcagcca gcaggacagc aacgtgtgca ggacctgctt gaatccctgc
     1921 caggcgacga gcgtcaacaa gatgggccag cggaccggga cgtatttgcc gagcgaccgc
     1981 aagccgtgct cgtagcaggc aatggcctgc tccatatcgc cccgtttgaa ggccagttcg
     2041 cccagcttcc cgcggatttc ggcctgggcg agcgtgccct cggcgtaatc gacggcggcg
     2101 ttgtattttt ttcccgcttc gtcatagcgg ccgcgcagca ggagcacttc gcccagcccc
     2161 agtgcgatgt ggtaccgcgt cgtcgcgtcg gcccagaaag cgccccgctc gcagatttcg
     2221 tactgctgct gggcagcttc tagggcgtag cgcgcgcggg cagtttttgc ggcttgcagg
     2281 gcgtagggaa gcgctgcgtg cggctggccg gcggcgtcga agtggaaggc gacctgcgcg
     2341 aactcgtcat cccggctttg caggtactga gcagcccggg cgtgcagcga ctgacgctcg
     2401 gcctgcggca gtcgcgcgag gagcgtttcg cgaatcttgt cgtggacgaa gacgcactgt
     2461 gcgccgccgg agcgcagcca cacgagctgc cgccgccggg ccacgtcgag ctgccgcagc
     2521 gccgtggcgg ggtcgagctc ggccagcgac accgccatat cgagatcgaa ctcttttccc
     2581 agaacagcgc ccgtggcgag tagcttctgc gtttctgcgg gcagcagttc caagcggcga
     2641 gccaggatct cggcggcccg gctggaggac tgcatttcgt ccaggtccag ggcttcgctc
     2701 cgccagccgc cgctgtcctg gaccagcttg cccgtttcga ccaggccccg cagcacagcc
     2761 gaggccatga acggactgtt ttcggcacag cggacaatgg ccaagaccac atcgtccggc
     2821 aggcggccgg ccatcgattc cagaagctgc cgcagctcat cggcggtcat gccggcgagc
     2881 cgcaggtggc tttcggtccg caggcgccgc agcggatgcc cctcagcgac ctcctcggac
     2941 cggaaggcca cgatgacgtg gacgtggcgg ttggccgttt cgctctccgc cggcgcgagc
     3001 caccgccgca gcaggcgata agtcaactcg tcggcccatt ggcagtcgtc cagaacgagc
     3061 agcagcggcc gagcgggcgt gcccaaagca ctcaggaagc ggtgcagagc gctgagcgtc
     3121 cgcatttcgc ccgccgcttc gggcgcggtc gagtaggagc tcgactggcc cagaatttcg
     3181 gccaggccgg ggagggcacc ggtcagggcg gaaagctgat cccccatccg ctcccggatg
     3241 ctggcgacaa gtcccggctg cgagcgggcc gccgccagga agccttggac gactcctgcc
     3301 aggagcgaga atggctgcgg ggcgacgtcg ctcgtgcctt gcccccacag gaaccagccg
     3361 ccgcgaccga gcgcgcgatt ggccgcctcg gtcaagaggc gcgtctttcc gccgccgctt
     3421 tcgccttcca ggagcgtcag tccgccgcgg ccctcctgcg cctgctcgac ttggcgctcc
     3481 agctcatgca gctcctcccg gcggccgacg aaggcgggct ccgagagtga ttgtcgtgtg
     3541 tcgtgcgctc cgacgacgat ggccggatcg tcctcgccac gggccacggc ggccgcaatc
     3601 gcattcaaat cggccagcac cgcttcggcc gactgatacc ggtcgcgcgg atccttccgc
     3661 agcagccgct gaatcacttc gtcgagtgct cgcggcacgg cgacgcccag cgccggcagc
     3721 cttggcgccg cc
//
LOCUS       DASUGB010000992         4143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1916053, whole genome shotgun sequence.
ACCESSION   DASUGB010000992 DASUGB010000000
VERSION     DASUGB010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916053"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFV87_18760"
     CDS             <1..1410
                     /locus_tag="VFV87_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HEX5105870.1"
                     /translation="VVTDGNGYYEFNGLRAGNYSVFEVHPQGFIDSIDTEGTTSGLAV
                     NIGTFVSPLVVQQFALLGVSFKFDAILQVPLAAGQHSQLNNFSEVQVTRIFIPPPEEP
                     PIEPPPPQIVLPQVPPEFLAPPIVIIPPKPAPLVTGGSGDWTWHLSVVDAGLPRISRK
                     GTRLTDIAWRPATSLEKIAWRPENLRAGQWTLPGSDPTDPPMLFGLPGAIPVVGDWNG
                     DGQDEIGIFYKGEWYLDLNGNGQWDASDLWAKLGSEADRPVVGDWDGDGKDDIGIYGP
                     EWPGDPRHLEHEPGLPDTENQRLHKPDPKNVPPDPEKATDGERLMRLTAKGKERADLI
                     DHVFRFGAPADLPIAGDWNGDGIRSVGIFRKGKWHFDMDGDGRWSEGDKIAHFGQEGD
                     IPVVGDFNGDGIEEIGVFRGGQWIIDTNGNHQIDAADQRIDLGAAGDQPIVGDFNGDG
                     LDDPALYRETAKDPEAPVE"
     gene            complement(1422..2135)
                     /locus_tag="VFV87_18765"
     CDS             complement(1422..2135)
                     /locus_tag="VFV87_18765"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HEX5105871.1"
                     /translation="MDRRKVLQLFSAGIGLPLAAAAGGDHHGAAAGASSTPLSEPHAH
                     FCGIHVAKRDPKFQLITQHYCCAHSQRGHEDLFQCVLFDRAGTGAKLLGVEYIVSDKV
                     YRELPDAEKRFWHAHTYEVLGGGLIAPGMSADEETQFMKSLLTTWGKVWHTWPDPASP
                     VPMGEPLLIWSLMGDGQVDEWLVAERDKQFGCSVDEIRQRRGRAIGYEAPKVAFPRSL
                     DTIGRQWTDQGDDRPTKRS"
     gene            2273..3457
                     /locus_tag="VFV87_18770"
     CDS             2273..3457
                     /locus_tag="VFV87_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105872.1"
                     /translation="MTRFIALWMLFASFPQVGGILAADESPLDAWQGNVKVSLVSPAE
                     RHSIHAYFNTSPESPDGRWVLFYTSALPEGHRGEIRIRERANGEEILLAGNVNVEDAH
                     RAACQQWLSGGKRVAFHTVSDDGECLVHVVEVASRETRVVARNRQLGFGQPLHDLLPL
                     YGPHWNPGECRGLELLNVATGELRSTPLTPDAIVAAYPEYIAKQFGERPVSLFFPLLS
                     PDASHVIFKLATPAGGSFRSAQASQREGLIGYDLKESRFLFQQDKWGHPAWHPNSRDV
                     LNTNGRVTDSDTGQVRLLPGRHRFPGSHPSYSPDGKLFTTDVQTSGEPFDGPAGSWAV
                     VVGDVATGEFVKLHQFDNAHGARSWRVSHPHPAFSPDGRRVYFNVSDGRWTRLFVAEG
                     SL"
     gene            3605..>4143
                     /locus_tag="VFV87_18775"
     CDS             3605..>4143
                     /locus_tag="VFV87_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5105873.1"
                     /translation="MDVSPQQDSPTLLAGGTLTSAGFIGLLATQFLTALNDNIFRWLV
                     IGIAKDHVTEDQVGNVLMGGTLGFVLPYLVLAAPAGYLADRHPKRSVILGCKVAEIVI
                     MALGIGAILCGGWGAAWALGLLMTTVALMGGQSAMFSPARAGSIPEVLKPELISKANG
                     LFTLATVIASVIGMVVGNAL"
BASE COUNT          725 a         1351 c         1320 g          747 t
ORIGIN      
        1 gtggtgaccg atggcaatgg ctactacgaa ttcaacggcc tccgagcggg caattactcc
       61 gtcttcgagg tccacccgca gggatttatc gacagcatcg acaccgaagg cactaccagc
      121 ggcctggcgg tcaacatcgg cacgtttgtc agcccgctcg tcgtccagca atttgctctg
      181 ctcggcgtga gcttcaagtt cgatgcgatc ctgcaggtgc cgctggcggc gggacagcac
      241 tcgcagctca acaacttcag cgaagtgcag gttacgcgaa tcttcattcc accgcccgag
      301 gagccgccga tcgagccgcc cccgccgcag atcgtcctcc cgcaagttcc gccggagttc
      361 cttgccccac cgattgtgat cattccgccc aaaccagcgc cgctggtaac cggcggctcg
      421 ggcgactgga cctggcactt gagcgtcgtg gacgccggcc ttccccgcat cagccgcaag
      481 gggacgcgcc tgaccgacat cgcctggcgg cccgccacgt cgctggaaaa gatcgcctgg
      541 cgtcccgaga atctccgtgc cggccagtgg acgctgcccg gcagcgaccc gaccgatccg
      601 ccgatgctgt tcggcctgcc tggggcgatt cccgtcgtcg gcgattggaa cggcgacggc
      661 caggatgaga tcggcatctt ctacaagggc gagtggtacc tcgatctcaa cggcaacggc
      721 cagtgggacg ccagcgacct gtgggccaag ctcggcagcg aagccgaccg gcccgtcgtc
      781 ggcgattggg acggcgacgg caaggacgac atcggcattt acgggcccga atggcccggc
      841 gacccgcggc acctggagca tgagccggga ttgcccgata ccgaaaacca gcgcctccac
      901 aagcccgatc cgaagaacgt gccccccgat cccgagaagg cgaccgacgg cgagcggctg
      961 atgcggttga cggccaaggg gaaggaacgg gccgacctga tcgatcacgt cttccgcttc
     1021 ggggccccgg ccgatctgcc gatcgccggc gactggaacg gcgacggcat ccgcagcgtt
     1081 ggcattttcc gcaaggggaa gtggcacttc gacatggacg gcgacggacg ctggtcggaa
     1141 ggggacaaga tcgcccactt cggccaggaa ggcgacatcc cggtcgttgg cgatttcaac
     1201 ggcgacggca tcgaggaaat cggtgttttc cgcggcggtc aatggatcat cgacaccaac
     1261 ggcaaccacc agatcgacgc cgccgaccag cggatcgacc tcggcgccgc cggcgaccag
     1321 cccatcgtcg gcgatttcaa cggcgacggc ctcgacgacc cggcactcta tcgcgagacg
     1381 gctaaggacc cggaagctcc cgtcgaatag ccccaggttg cctaagatcg cttcgtcggc
     1441 cggtcgtcgc cctggtcggt ccattggcgt ccgattgtgt cgagcgagcg tgggaaggca
     1501 accttcggcg cttcgtaacc gatggcccgc ccgcggcgct ggcggatttc gtccaccgag
     1561 caaccgaatt gcttgtcgcg ctcggcgacc agccactcat ctacctgccc atcacccatg
     1621 agcgaccaga tcaagagcgg ttcgcccatg ggaacgggcg aagccggatc gggccaggta
     1681 tgccagactt tcccccatgt tgtgagaagc gacttcatga attgcgtttc ttcgtcggcc
     1741 gacatacctg gcgcgatgag cccgcccccg agcacttcgt acgtgtgggc atgccagaac
     1801 cgcttctccg catcgggcag ctcgcgatag accttgtcgc tgacgatgta ttccacgccc
     1861 agcagcttcg cgcccgtgcc cgcacggtcg aagagcacgc actggaacag gtcctcgtgc
     1921 ccgcgctgcg agtgggcgca gcagtaatgc tgcgtgatga gctggaattt cgggtcgcgc
     1981 ttggcgacgt gaattccgca aaagtgcgcg tgcggctcgg acagcggcgt gctggacgcc
     2041 cccgctgcgg ccccgtgatg atctcccccg gccgccgcgg ccaaaggcag cccgatcccg
     2101 gcgctgaaga gttgcagtac cttgcggcgg tccatagcga gcctcctgga agtgacgaag
     2161 tcttcgcctg gaactagatg tcgccttaac ggggcgacct ttcgcagaat tctaggcagc
     2221 aacgggttag aatcacctgg gattgaattc ctcgccgcga ggtagccgtt tcatgacgcg
     2281 atttattgcc ctatggatgt tgttcgcctc gtttcctcaa gtgggaggga tcttagccgc
     2341 agatgaaagc ccgctggacg cctggcaggg caatgtgaag gtctcgctgg tttcaccggc
     2401 tgagcgccat tcgatccacg cgtacttcaa cacgtcgccc gagagcccgg acggccgctg
     2461 ggtgctcttc tatacgtccg ccctgcccga ggggcaccgt ggcgagattc gcatccgcga
     2521 gcgggcgaac ggcgaggaga ttctgctggc cgggaacgtg aacgttgagg acgcccaccg
     2581 cgccgcttgc cagcagtggc tcagcggcgg caagcgcgtc gcgtttcaca cggtctccga
     2641 cgatggcgag tgcctggtcc acgtagtcga agtcgcctcg cgcgagacgc gggtcgtggc
     2701 tcgcaaccgg cagctcggct ttggccagcc gctgcacgac ttgttgccgc tgtacggccc
     2761 gcactggaat ccgggcgagt gtcgtggcct ggaactactg aatgtcgcaa ccggcgaact
     2821 ccgctcgacg ccgctgacgc cggacgcgat cgttgcggca tacccggagt acatcgcgaa
     2881 gcaattcggc gagcggcccg tctcgctctt cttcccgctg ctcagccccg acgccagtca
     2941 cgtgatcttc aagctggcga cgcccgccgg cggcagcttt cgcagcgcgc aagcgagcca
     3001 gcgtgaaggg ctgatcggct acgacctgaa agagtcccgg ttcctcttcc agcaagacaa
     3061 gtggggccat cccgcctggc acccaaattc gcgtgatgtg ctcaacacca atggccgagt
     3121 aacggattcc gatacgggcc aggtccgctt gcttcctggt cggcaccgct tccccggcag
     3181 tcacccgtcg tatagcccgg acggcaagct gttcacgacg gacgtgcaaa cgagcggcga
     3241 gccctttgac ggccctgctg gctcgtgggc cgtcgtagtc ggcgacgtcg cgaccggcga
     3301 gttcgtcaag ctccatcaat tcgacaacgc ccacggcgcg cgctcctggc gcgtcagcca
     3361 tccgcatccc gccttcagcc ccgatggccg gcgcgtttac ttcaacgtca gcgacggccg
     3421 gtggactcgg ctgttcgtgg ccgaaggttc gctctgagct atctttacgt ggatgcgtgg
     3481 cgcacacgtc ctggcgagta aagccgcgca gatcgcagag tcgcgctgtg ttggttgcag
     3541 ttgctgggct atattttagc cagtctacgc tccgctcagg ccaatcttct cgacgtgcag
     3601 cttcatggat gtttctccac agcaggactc gcccacttta ttggctggcg ggactttgac
     3661 gagcgccggc ttcatcgggc tgctggcgac gcagttcctc acggcactca acgacaacat
     3721 cttccgctgg ctggtgatcg ggatcgcgaa ggatcacgtc acggaggacc aggtcggcaa
     3781 cgtgctgatg ggcggcactt tgggatttgt gctgccctat ctggtgctgg ccgctcctgc
     3841 gggatacctg gccgaccggc atcccaaacg aagcgtgatt ctgggctgca aagttgccga
     3901 gatcgtgatc atggcgctgg gcattggggc gatcctttgc ggcggctggg gtgcggcgtg
     3961 ggcattgggc ctgttgatga cgacggtggc cctgatgggc ggccaaagcg ccatgttctc
     4021 tccggcgcgg gctggcagca ttccggaagt cctgaagccg gaactgatca gcaaggccaa
     4081 cggcctgttc acgctcgcga cggtcattgc cagcgtcatt ggcatggtgg tcggcaacgc
     4141 cct
//
LOCUS       DASUGB010000993         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1083229, whole genome shotgun sequence.
ACCESSION   DASUGB010000993 DASUGB010000000
VERSION     DASUGB010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083229"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(468..746)
                     /locus_tag="VFV87_18780"
     CDS             complement(468..746)
                     /locus_tag="VFV87_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX5105874.1"
                     /translation="MYIYGTAHVHAPQPINAPHRTAPAAAPTSSPYAGGVDQLDLSPE
                     AEFVAQARDLPEIREDRVAAIRAQIEAGTYETPEKLDIALSRMLDEIA"
     gene            1764..>2911
                     /locus_tag="VFV87_18785"
     CDS             1764..>2911
                     /locus_tag="VFV87_18785"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5105875.1"
                     /translation="MRNFVLGLFAAAVACGAGPLLAQEEADLFTKLDANKDGFVTADE
                     VGEDNQAKFERQLRLADKDGDKKLSKEEFAASLRPDDAPRQPLGAGGQPGRPGRPGGF
                     PQFNPRAIFAQMDANKDGKLAKDEVRGPLAEGFDRADANKDGFISEEEFAEVSRRFGG
                     GRPGADPRQVEELFARWDANSDGKITKDEVPEPRQQAFAQVLERLSADSVSKEQFARV
                     AQFLAPPGGPGDRRPDGRPGETRPEGRPGEPRPDARPGEPRPDGRPGEPRPGGPGFRP
                     PLVAALDADGDGELSAEEIEAAGKSLLKLDRNGDGKLTTDEFGPPRPDARPGEGRPDA
                     RPGEGRPNGLVEFLRASDTNKDGKISKDEAPDRVREAFDRFDANSDGF"
BASE COUNT          536 a          890 c          967 g          518 t
ORIGIN      
        1 catccagacg tgggagagtt gggtatcgat gacgtgcatg aggtcgatag gggacatgct
       61 tgcattgtcg ttccgtcgag cgaagattcc agccgaagac gtgacaacca gcagctcaga
      121 gcacctcgct cactacaata agccccaccg gttgtactgg cggtgtcggt gccagcgcgg
      181 agggcgttcc aggcgaggac catgtcgtac acgcgttgga agccgcgatg ggggtagttg
      241 gtgaatccgc cgagctgcgc gatgagggtt ccgcctgcca gcggcacttc ctcgaccggt
      301 gcgagttcct cggccaagaa tgcccgccgc cgttaatccg gaaggaccat agtatcggta
      361 ttactaaggc caagctgctc gcgctgaatt tcgcgcgagc tgcagaagga tcctattcct
      421 cctcctgctc ctcagtgggc cgatcccggg aacaggagaa ggacaaatta cgcaatctca
      481 tccagcatgc gcgagagcgc gatgtccagc ttctcaggcg tctcgtacgt ccccgcttcg
      541 atctgggcgc ggatcgcggc gacgcggtcc tcgcggatct cgggaagatc gcgggcctgg
      601 gcgacgaatt cggcttcggg cgaaaggtcc aactgatcga ctccgccggc gtacggcgag
      661 ctggttggcg cggcagccgg cgccgtgcgg tgcggcgcgt tgatcggctg gggggcgtga
      721 acgtgggcag ttccgtaaat atacattgca ggtctctcct tggcctgcca ggcgggcgat
      781 tgtcggccgg ccacaggcat tctcccttgg atcgaaaaac tggagaagag cgatcctgcc
      841 cgccagccgt cggaccgtac actgcccgag cgggctaagg aagtgcacgc ggctggcgtc
      901 aagctggact gcttcatccc cgaaacgtga aaccaaaccc aatccgagcg tcgcgatccg
      961 tgtcggggga gcgtccgtga gtagcgaacg gcgaccttgc gactgctagc acgttagcac
     1021 cccatacatc ggcccgcgga tggcggtcaa tcaagccaaa atggcccggc aacctcctgt
     1081 gccggtttct tatgtcttaa gcgcctgtgc cgggcgtgcc tgccgggaat ttgctgcggc
     1141 tggcggcagt tgtgcggtcg gctttggagg ggttctccgt tgccggcggg caactgcggt
     1201 gcatccgctt ccaggcatgt tgctttgggg caacattggc tgaatgaggt caggtgaagg
     1261 actccttccc ggctggtcct cagcggaccg agactcctgt ttgggccgag gcttcattcc
     1321 agattccaac gctgtggcgg tagctggcgc ctctgtgcca cagtcaccct acgcggggac
     1381 atggccgatg gtttgggctg gcaaagattt tttgccgccg gccacctgct gctgcgcaaa
     1441 ccggtgccgg cggcggagta taggccagaa gctcaccgcg cgacaacgtc gacgtaaggc
     1501 gagaattcac aacgccttgg gccgtctggt agcatgctgg ctggcgtatt gcggtaacag
     1561 gctgctacgg cgcggctaat cctaggaggt gcgccgtccg caagactgaa ctgcattaaa
     1621 tccgcacgct ggagcgttga gttgcgagcg ggaatcgccg cctgccgcgg aacatgtgcc
     1681 gtccacaacg gtttacaccg tagccatacg aacacgtttc tttgctggcg aagttcgccg
     1741 gccacccttt cggagactct cccatgcgca atttcgttct gggtctattt gcggccgctg
     1801 tcgcgtgcgg cgctgggccg ttgctcgcac aggaagaagc cgacctgttc accaaattgg
     1861 atgccaacaa ggacggcttt gtgacggctg acgaggttgg cgaggacaac caggccaagt
     1921 tcgagcggca gctccgcctg gccgacaagg acggcgataa gaagctgagc aaagaggaat
     1981 ttgccgccag cctgcggccc gatgatgccc cccgccagcc gctgggcgcc ggcggccagc
     2041 ccggaaggcc aggccggccg ggcgggttcc cgcagttcaa cccgcgggcg atcttcgcgc
     2101 agatggatgc aaacaaggat ggcaagctgg ccaaggatga ggtgcgcggg ccgctcgccg
     2161 agggcttcga ccgcgcggac gccaacaaag acggattcat tagcgaagaa gagttcgctg
     2221 aggtttcgcg tcgattcggc ggcggccgtc ctggggccga cccgcggcag gtcgaagagt
     2281 tgttcgcccg ctgggacgcg aatagcgacg gcaaaatcac gaaagacgaa gttcccgagc
     2341 cgcggcaaca ggcgttcgcg caggttctcg agcggttgag cgccgattcg gtcagcaaag
     2401 agcagtttgc acgtgtggca cagtttttgg cgcctcccgg aggccctggt gatcgccgcc
     2461 ccgatggccg tcccggcgaa actcgtcccg agggtcgccc cggtgagccc cgaccagacg
     2521 cacgtccggg tgaaccccgg cctgacggtc gccccggtga gccgcggccc ggtggtcccg
     2581 gcttccgtcc gccgctggtc gctgctctcg acgccgacgg cgatggcgaa ctttcggctg
     2641 aggaaatcga agctgcgggc aagtctctgc tcaagctcga tcgcaacggc gacggcaagc
     2701 tgacaaccga cgagtttggc ccgccgcgac ccgatgcccg ccccggcgaa ggacgtcccg
     2761 acgcccgacc cggcgaaggc cggcccaatg ggcttgtgga gtttctgcgg gcctccgaca
     2821 ccaacaaaga tggcaagatc agcaaggacg aagctcccga tcgcgtgagg gaggcctttg
     2881 accgcttcga tgccaacagc gacggattca t
//
LOCUS       DASUGB010000994         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_805131, whole genome shotgun sequence.
ACCESSION   DASUGB010000994 DASUGB010000000
VERSION     DASUGB010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805131"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFV87_18790"
     CDS             <1..1055
                     /locus_tag="VFV87_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5105876.1"
                     /translation="SRLKSSFFQDSPDHRILNTALALQKREHPRPVMLVSKDTNLRMK
                     AKSLGVPSQDYTSDKIESFDKLYTGKRVIEDAPGETISRFYENGGHVPASEVACVSHP
                     LPNENFILRSGSKSVLATYRAAEEKFVRVEKVNTYGVSPRNAEQSFALKALTNDDIKL
                     VTLTGTAGTGKTILALAAALECRQRYRQILLARPVVPLGNRDLGYLPGDIEAKLDPYM
                     QPLYDNLTVIRHQFNDSDPAAQRIIEMRENEKLLITPLAYIRGRSLQRIFFIIDEAQN
                     LTPHEVKTIITRAGEGTKIVLTGDIKQIDQPYLDALSNGLSYLINRMVGQPIFAHITL
                     EKGERSALADLASELL"
     gene            complement(1125..1637)
                     /locus_tag="VFV87_18795"
     CDS             complement(1125..1637)
                     /locus_tag="VFV87_18795"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX5105877.1"
                     /translation="MDEPRKERLRPLVASCFGLGYSPIMPGTCGALIGPVIYIPLALL
                     VPGWVLQTALIGVALVVWSWITVALGSWAEDYYQKKDSQTFVTDEVAGFLLTVLLFHL
                     PEQPVLTTLWAFPVTRIIDMIKVPPARQLEKLPGGWGVLADDLLGSVYAAGLLWMVWL
                     AMPAWFGGSA"
     gene            complement(1679..2980)
                     /locus_tag="VFV87_18800"
     CDS             complement(1679..2980)
                     /locus_tag="VFV87_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105878.1"
                     /translation="MSHQMHIQSDVSIRRGLVQRRDFLRAIPAAALAAGALSWQDALI
                     ASAADLRRSGKACILLFMQGAPSQFETFDPKPGHANGGETKAIATSVPGIEICENLPQ
                     TAQVMEHLCLIRSMTSREGAHPRAQYLMHTGYLPTATVKYPTLGSIVAHELGDPASEL
                     PSFVRIGGRGLGNDGAGLLGVAYDPLAMGRAGQPPDNTALTTPAARYQRRLNLLTRLE
                     ADYANQGGEQEVADHQKIYGQAAKMVTSPKMAAFSIDKEPAKVREAYGSGAFGQGCLL
                     ARRLIESGVTFVEVGLGNWDTHQDNFNRTKTLCGQLDQPLAALIGDLKDRGMLGSTLI
                     VWTGEFGRTPRINANSGRDHYPRAFNAFLAGGGVKGGQVIGGTSAGGDTPTGRTVSVN
                     DLLRTVCTSLNIDPSKENMSPIGRPIKIVDGGEVVKEVFGA"
BASE COUNT          573 a         1088 c          858 g          498 t
ORIGIN      
        1 acagccggct caagtcctcg ttctttcagg attcgcccga ccaccgcatt ctcaacacgg
       61 ctctggccct gcaaaagcgc gagcatccgc ggccggtaat gctcgtcagc aaggacacca
      121 acctgcggat gaaagccaag agcctgggcg tcccgtcgca ggactacacc agcgacaaga
      181 tcgaatcgtt cgacaagctc tacaccggca agcgcgtcat cgaggatgcg ccgggcgaga
      241 cgatttcgcg gttctatgaa aacggcggcc atgtgccggc ttccgaagtg gcgtgtgttt
      301 cccatccgct gccgaacgag aacttcatcc tgcgctcggg cagcaaaagc gtgctggcga
      361 cctatcgcgc cgcggaggag aagttcgtcc gcgtcgagaa ggtcaatacg tacggagtct
      421 caccgcggaa cgccgagcaa tcgttcgctt tgaaggccct gaccaacgac gacatcaagc
      481 tcgtcacgct caccggcacc gcgggcaccg gcaagacgat cctcgcgctc gccgccgccc
      541 tcgaatgccg ccagcgctac cggcagattc tgctggcgcg gcccgtggtg ccgctgggca
      601 atcgcgatct cggctacctg cccggcgaca tcgaggccaa gctcgatccc tacatgcagc
      661 cgctgtacga caacctcacc gtgatccgcc accagttcaa cgacagcgat ccggccgccc
      721 agcggatcat cgagatgcgc gagaacgaga agctgctcat cacgccgctg gcctacatcc
      781 gcggacgcag cctgcagcgg atcttcttca tcatcgacga ggcccagaac ctcacgccgc
      841 acgaagtgaa aacgatcatc acgcgcgccg gcgaagggac gaagatcgtc ctcaccggcg
      901 acatcaagca gatcgatcag ccgtacctcg acgccctctc gaatggcctc tcctatctca
      961 tcaaccgcat ggtcggccag ccgatcttcg cccacatcac gctggagaag ggcgaacgca
     1021 gcgcactggc cgacctggcg agcgagctgc tgtagggcgg gaccagcccg catagcgggc
     1081 gcagttttgc cggtgggcat gcggcctgac ggccttgtcc caccctacgc ggaaccgcca
     1141 aaccacgccg gcatcgcaag ccacaccatc cacagcaacc ccgccgcata caccgagccg
     1201 agcaggtcat cggcgagcac gccccagccg ccgggcagct tctcgagctg cctggccggc
     1261 ggcactttga tcatgtcgat gatccgggtc acggggaatg cccacagcgt cgtcagcacg
     1321 ggctgctcgg gcaggtgaaa cagcagcacg gtgagcagga acccggcgac ctcgtcggtc
     1381 acgaacgtct ggctgtcttt cttctgatag tagtcctccg cccaactgcc cagcgcgacc
     1441 gtaatccagc tccacacgac gagcgccacg ccgatcagcg ccgtctgcag gacccagccc
     1501 ggcacgagca gcgccagcgg aatgtagatc accggaccga tcagcgcccc gcacgtgccg
     1561 ggcatgatcg gcgaatagcc caatccgaaa cagctcgcaa ccagcgggcg caatcgttcc
     1621 tttcgcggct catccattcg gccagcgtac cactcaccac tcactactga ccactcacct
     1681 acgccccaaa aacctccttt accacctccc cgccatcgac aatcttgatc ggccggccga
     1741 tcgggctcat gttctctttg ctcggatcga tgttcaggct cgtgcaaacc gtccgtagca
     1801 agtcgttgac gctgaccgta cggccggtcg gggtatcgcc gccggcgctg gtgccgccga
     1861 tcacttgccc gcctttcaca ccgccgccgg ccaggaaagc attgaacgcc cgcggatagt
     1921 ggtcgcggcc gctgttggca ttgatccgcg gcgtgcggcc gaattcgccc gtccacacga
     1981 tcagcgtgct gcccaacatt ccgcggtcct tcaggtcgcc aatcagcgcg gccagcggct
     2041 ggtcgagctg gccgcatagc gttttggtgc gattgaagtt gtcctggtgc gtgtcccagt
     2101 tgcccaggcc cacctcgacg aacgtcacac ccgattcgat aagccgccgc gcgagcaggc
     2161 acccctggcc aaacgccccg ctgccgtagg cttcgcggac tttggcaggc tccttgtcga
     2221 tcgagaaggc agccatcttc ggactggtaa ccatcttcgc cgcctggccg tagatcttct
     2281 gatgatcggc gacctcctgc tcgcccccct ggttcgcgta gtcggcctcc agccgcgtca
     2341 gcaaattcaa ccgccgctgg tagcgggcag ccggcgtcgt caaagcggtg ttgtccggcg
     2401 gctgacccgc gcggcccatc gccaacgggt cataagcgac tcccagcagg cccgccccat
     2461 cgttccctag tccccggccg ccaatccgca cgaagcttgg cagctcgctc gccggatcgc
     2521 ccagctcatg ggccacgatc gagcccagcg tcggatactt caccgtcgcc gtcggcagat
     2581 agcccgtgtg catcaggtac tgcgcccgcg gatgcgcccc ctcgcgactg gtcatcgagc
     2641 ggatcaggca caagtgctcc atcacctggg cggtttgggg cagattttcg caaatctcaa
     2701 taccgggcac cgaagtggcg atcgccttcg tctcgccgcc attggcatgc cccggtttgg
     2761 gatcgaacgt ctcgaactgg ctcggcgcgc cctgcatgaa cagcaggatg cacgccttgc
     2821 cgctccggcg caggtccgcc gcgctggcaa tcagcgcgtc ctgccagctc agcgctcccg
     2881 cagcgagcgc cgcagccgga atcgcgcgca gaaaatcccg ccgctgcacc agcccgcggc
     2941 gaatggaaac gtcggactga atatgcatct gatgcgacat ctcaaccgct ccttacttac
     3001 accactcacc gactcac
//
LOCUS       DASUGB010000995         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1916462, whole genome shotgun sequence.
ACCESSION   DASUGB010000995 DASUGB010000000
VERSION     DASUGB010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916462"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV87_18805"
     CDS             complement(<1..336)
                     /locus_tag="VFV87_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105879.1"
                     /translation="MTRRLARQLRTVIRKLLGNVRPDVLVRTGDFGLLAQVQGYEHAV
                     QYRDPAPRRLAELMVPFAVLDDVQGPKIEPVVLTKPKKDVLAASWEDRGVAQSIQYDL
                     PQARSDAFPT"
     gene            complement(432..620)
                     /locus_tag="VFV87_18810"
     CDS             complement(432..620)
                     /locus_tag="VFV87_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105880.1"
                     /translation="MTQQQLDREVASKLGESVGTIRRHGFSLVTPLTIFDPDAEELAQ
                     PQVLDWDQVQAERCARAA"
     gene            complement(722..967)
                     /locus_tag="VFV87_18815"
     CDS             complement(722..967)
                     /locus_tag="VFV87_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105881.1"
                     /translation="MQLVIEPSGGVRCLYGETIDLAALGALTISRGSHVEPTAGGQWL
                     CDLSPVTGPVLGPFDCRSQALAAEVAWLEAHWLAPAD"
     gene            complement(971..1942)
                     /locus_tag="VFV87_18820"
     CDS             complement(971..1942)
                     /locus_tag="VFV87_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105882.1"
                     /translation="MPTLLDPPAPVAGNSTHLTQAAAERLRSRMAAVRLAFVWFGVRK
                     TLTQEQKSQAADTFGAEGEYLSAAKKLLDTSHPAYRAVTGVKSRIVAYWRGISLPYPE
                     PGIRLIRQDDVASFSVQLTTLQAELVEAVEQLSSQYQELKAAARQRLGRLFNGADYPA
                     TLVGLFEASYDFPSFEPPDYLRQLSPQLYAQEAQRVAARFEEAVQLAEQAFTEELSKV
                     VSHLTDRLSGSEDGKPKVFRDSAVENLQEFFQRFRHLNVRSSQQLDDLVDQAQRIVLG
                     VQPQQLRDNTDLRQQIAAQLSGVQSVLDGLLVDRPRRNILRRQRQDG"
     gene            complement(2037..2570)
                     /locus_tag="VFV87_18825"
     CDS             complement(2037..2570)
                     /locus_tag="VFV87_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105883.1"
                     /translation="MSEPIPACISIGGKLPRHLVPQLCESIQADGLSLEWGDAHFRPK
                     TAEDLLDACRDERGVSILWLCDDQADWGRLPNLEDFLTKVGKLPFDLRSEGKMSFDPD
                     LVRHRPGKRPVQITTNAQGDPVVIAQLLAPVVKTLAKAADAKSRQDCQADVQRALRRL
                     RSVLPPKIAPLPPFEIG"
     gene            complement(2598..3254)
                     /locus_tag="VFV87_18830"
     CDS             complement(2598..3254)
                     /locus_tag="VFV87_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5105884.1"
                     /translation="MSISAPRFERQADLVPRDKLAKLTVTVIGVGAIGRQVALQLAAL
                     GVRQLQLIDFDQVEPTNITTQGYLEANLGWRKVEATASAARQIDPEITVTLVEDRYRP
                     QHKLGEAVFCCVDSIDARSAIWRTAGRRCRFWCDGRMLGEVMRILTVAEEQGRAHYPT
                     TLFQQAEAQTGACTSRGVIYTAAIAAGLMLHQFTRWLRELPTDADLSLNLLASELAAG
                     "
     gene            complement(3229..>3428)
                     /locus_tag="VFV87_18835"
     CDS             complement(3229..>3428)
                     /locus_tag="VFV87_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105885.1"
                     /translation="EPVVVESRRNRLLLGPADELENRAPQVWWNHPLWDGLPQLASPF
                     DDEFSFHPHPETEDEYFGAPF"
BASE COUNT          607 a         1088 c         1164 g          569 t
ORIGIN      
        1 cgtgggaaac gcgtcgctcc gggcctgggg caggtcgtat tgaatcgact gggcgacgcc
       61 gcggtcctcc cagcttgccg ccaagacatc cttcttgggt ttggtcagaa cgacgggttc
      121 gatcttgggg ccctggacat cgtccaggac ggcgaagggg accatcagct cggccaatcg
      181 gcggggcgcc gggtcgcggt actgcacggc gtgctcgtag ccctggacct gggcgagcag
      241 cccgaagtct ccggtgcgga ccagcacgtc ggggcgcacg ttgccaagca gcttgcgaat
      301 gaccgtccgc agctgcctgg ccaatcgtct cgtcatctca atcaaggcga acgctcctat
      361 agggaaggga caaaagaact gggcggcaac ccttgctgcc gcccctagga ttttgctgaa
      421 cagacaatcg gtcaggccgc ccgcgcgcag cgctcggctt ggacctgatc ccagtcgagg
      481 acctgcggct gggccagttc ctcggcgtcg ggatcaaaga tcgtcagcgg cgtgacgagc
      541 gagaacccgt gccggcggat cgtgccgacc gattcgccca gcttggaggc gacttcccgg
      601 tccagttgct gttgggtcat tgcagacatt cctttctgta gcaagaacta aaaacggccg
      661 gccaagcgct ggcgcgcagg gccggccgga aaactcactc aagtggactc agcgagcggc
      721 gctaatcggc tggagccagc cagtgcgctt cgagccaggc aacttcggcg gccagcgcct
      781 ggctgcggca gtcaaagggc ccgagcacgg ggccagtgac cggcgacaag tcgcaaagcc
      841 actggccacc ggccgtgggc tccacatgcg agccgcggga gatggttagc gcgcccaggg
      901 cggccaggtc gatcgtttcg ccgtagaggc agcggacccc gccgctcggt tcaatgacca
      961 gttgcatggc tcagccatcc tgccgctggc ggcggaggat attgcggcgg ggccgatcga
     1021 ccagcaggcc atccaggacc gactgcacac cggaaagctg agcggcgatc tgctgacgga
     1081 gatccgtatt gtcacgaagt tgctgcggct ggacccccag cacgatccgc tgcgcctggt
     1141 cgaccaggtc atcaagctgc tggctggagc ggacattcag gtgccggaac cgctggaaga
     1201 actcctgcag gttctcgacc gccgagtccc gaaagacctt gggcttacca tcctcgctac
     1261 cggacaggcg gtcggtcagg tggctcacca ccttggataa ctcttcggtg aaggcctgtt
     1321 cggccaactg caccgcctct tcgaaccggg cggccacgcg ctgggcctcc tgggcgtaca
     1381 gctgcgggct gagctgccgc aggtaatccg gcggctcgaa ggagggaaag tcgtaactgg
     1441 cctcaaagag gccgacgagc gtggccgggt agtcggcgcc attgaagagc cgccccaggc
     1501 gctggcgggc agccgctttc agttcctgat actggctgct cagctgctcg accgcttcga
     1561 ccagttccgc ctgcagcgtc gtgagctgga cggagaagct ggccacgtcg tcctggcgga
     1621 tcaggcggat gccgggctcc gggtaaggaa gcgagatccc gcgccagtag gcgacgattc
     1681 ggctcttgac gccggtgacg gcccggtagg ccggatggga cgtatcgagc agcttcttgg
     1741 cggccgagag gtattccccc tcggcgccga acgtatcggc ggcctgggat ttttgttctt
     1801 gggtgagcgt cttgcgcact ccaaaccaga caaacgccag ccgtacggcc gccatccgcg
     1861 agcggagccg ctcggcggcg gcctgggtca ggtgtgtcga attgccggcc acgggagccg
     1921 gcggatcaag gagtgtgggc atgcagggct ccgaaaggag atgggggacg ggattcacga
     1981 aatcagcgga gccgggagcg ccgctggtaa caaatccgaa cgactattga gcgcgcctag
     2041 ccaatctcga acggcggcag cggcgcgatc ttaggaggca gaaccgaccg caaacgcctc
     2101 aaggcacgct ggacgtcggc ctggcaatcc tggcggctct tggcgtctgc cgccttggcg
     2161 agcgttttga cgaccggcgc caagagctgg gcgatcacca cggggtcgcc ctgggcattc
     2221 gtcgtgatct gcacgggccg tttgcccgga cggtgtcgga ccaggtccgg gtcaaagctc
     2281 atttttcctt ccgatcgcag gtcgaagggg agtttcccga ccttcgtcag aaaatcctcc
     2341 aggttgggca ggcggcccca gtcggcctga tcgtcacaga gccagaggat gctgacgccc
     2401 cgctcgtcgc ggcaggcgtc cagcaggtct tccgccgtct tcggtcggaa gtgcgcgtcg
     2461 ccccattcca gggatagtcc gtcggcctgg atcgattcgc acaactgcgg aacgaggtgg
     2521 cgagggagct tgcctccgat cgagatacaa gcgggaatcg gctcggacat ttttcttggg
     2581 aatctccgga acgagggtca accagccgcc agttcgctgg ccagcaggtt cagggacaga
     2641 tcggcatcgg tcggcaactc gcggagccag cgggtgaact ggtggagcat gagccccgcc
     2701 gcaatggccg cggtgtagat caccccgcgg ctggtgcagg cgccggtctg ggcttcggcc
     2761 tgctggaaaa gagtcgtcgg atagtgggcg cggccttgct cctcggccac ggtcagaatc
     2821 cgcatcactt cccccagcat ccggccgtcg caccagaacc ggcagcggcg gccggcggtg
     2881 cgccagatgg ccgagcgggc gtcgatcgaa tcgacacagc agaagaccgc ctcgcccagc
     2941 ttgtgctgcg gccggtagcg atcttccacc agcgtgacgg tgatctcagg atcgatctgg
     3001 cgcgcggcgc tggccgtcgc ctcgaccttg cgccagccca agttcgcttc gagatagccc
     3061 tgcgtcgtga tattggtcgg ttcgacctga tcgaagtcga tcagctggag ctggcgcacg
     3121 cccagggcgg ccagctgcag ggccacctgc cggccgatcg ccccgacgcc gatcacggtc
     3181 acggtcagct tggccagctt gtcacgcggc accaagtccg cctggcgctc aaaacggggc
     3241 gccgaaatac tcatcttcgg tctccggatg gggatggaag gaaaactcat cgtcaaacgg
     3301 gctggccagc tgcgggaggc cgtcccacag cggatgattc caccaaacct gcggcgcccg
     3361 attctccaac tcgtccgccg ggccgaggag cagccggttg cggcgggact cgacaaccac
     3421 tggctctg
//
LOCUS       DASUGB010000996         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_805393, whole genome shotgun sequence.
ACCESSION   DASUGB010000996 DASUGB010000000
VERSION     DASUGB010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805393"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VFV87_18840"
     CDS             <1..1385
                     /locus_tag="VFV87_18840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5105886.1"
                     /translation="LVVYTDPDRKLVRAFAVDRDGETFKVTSQFDLMTSDDPLFRPCQ
                     AVQGPDGAIYIADWRTDSPRGDRLTGDGQQGRIYRLSWGGSDDYAEIPLAPLDAWQQI
                     AAAGEDQLTSLLSSDEFELRRRAALELVRRGQLDESAGPAIAARAATIAYDDSRRPVV
                     RAAAIAVAGQLTDATAFEALLAMTSDENPDIQRLAATALGENLPKSDEHRRRLIEALR
                     EHLFAQKPGVPRAMFLAHGKAARSVDDAEWAFEATSVTYQPSMGPHLFDAHVRALEMT
                     KDAARELLLGNLDVALNFEVADTKERERLKLFVASNAEAMRTRELAVFLDALFTGEDD
                     LLAKLAPPLQARLIACYRNVQVEPPINTDALATWLEKHPGLPAEVEIAALETMSLVRP
                     SQPEPLTKLAERLLANADHTKEIARRLIAGSLDRKLQPGVIEALRRHSERDSTGECKT
                     LLNQLLTPDS"
     gene            complement(1408..2007)
                     /locus_tag="VFV87_18845"
     CDS             complement(1408..2007)
                     /locus_tag="VFV87_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HEX5105887.1"
                     /translation="MIRIDEITTLQRECVSRWHQEPVDQPHEGLLGLVCQQHAFNFRL
                     WHEEDKARSREAGDSQIAAVKRAIDQLNQQRNDWIERLDDWITEALANQCILAPAVAP
                     QNTETLGSTIDRLSILALRIYHLAEERDRPETTPEHREAVGRKLAVALAQLDDLSLAA
                     QQLADDLFAGRKRHKTYRQLKMYNDPALNPYLYRALKVA"
     gene            2168..>2991
                     /locus_tag="VFV87_18850"
     CDS             2168..>2991
                     /locus_tag="VFV87_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5105888.1"
                     /translation="MSRTAEPRSTSRAPRPSGTVWFYVLLLAAGGLALAGIATINSGS
                     SAQRPAPSNLTLDDIPVNGERAYGYLKEICAIGPRVSGTEGMRLQQEYLQAHFEKLGG
                     KVSRQEFAARHPQTGARALMANLIVEWHPERTDRILLCAHYDTRPFPDQDPVDPKGLF
                     LGANDGASGTALLCELAHDMADVKGKLGVDFVLFDGEELVYDGQRDPYFLGSEHFSRE
                     YLANPPGHRYKYGVLLDMVADSNLAIYREVNSMRTRETRQLVNDVWDVARGLGVRE"
BASE COUNT          512 a         1010 c          953 g          516 t
ORIGIN      
        1 gccttgttgt ctataccgat cccgaccgca agctggtccg cgcgtttgcg gtcgatcgcg
       61 acggcgagac gttcaaggtc accagccagt tcgacctgat gacgagcgac gatccgctct
      121 tccggccgtg ccaggcggtg caaggccctg atggcgcgat ttacatcgcc gattggcgca
      181 cagatagccc gcgcggcgac cggctgacgg gcgacggcca gcagggccgc atctatcgct
      241 tgtcgtgggg cgggagtgat gactacgcgg aaattccgct ggcgccgctc gatgcgtggc
      301 agcagattgc agccgcgggc gaggatcagc tcacgagcct gctcagcagc gacgaattcg
      361 agctccgccg ccgggctgcg ctggagttgg ttcgccgcgg ccagcttgat gaatcagccg
      421 gcccggccat cgccgcccgc gcggcaacga tcgcctacga cgacagccgc cggcccgtcg
      481 tccgcgccgc ggccatcgcc gtggcgggcc agctcacgga cgccacggca ttcgaggcct
      541 tgctggcaat gacgagtgac gagaatccgg acattcagcg gctggcagcg acggccctgg
      601 gcgagaattt gcccaagagc gatgagcatc gccgccgcct gatcgaagct ttgcgcgagc
      661 acctcttcgc ccagaagccg ggtgtgccgc gggccatgtt tctggctcac ggcaaggccg
      721 cccgcagcgt ggatgacgcc gagtgggcct ttgaggcgac cagcgtgacc tatcagccga
      781 gcatgggtcc gcacctcttc gacgcgcacg tgcgggcctt ggaaatgacc aaagacgccg
      841 cccgcgaact gctgctcggc aacctcgacg tagcgctaaa tttcgaggtc gctgacacca
      901 aggaacgcga gcggctgaag ctgtttgtcg ccagcaatgc cgaggcgatg cggacgcgcg
      961 agctggccgt ctttctcgat gcgctcttca ccggcgaaga cgacctgctg gccaagctcg
     1021 ccccgccgct gcaggcccgc ctgatcgcct gctatcgcaa cgtgcaggtc gagccgccga
     1081 tcaacaccga cgccctcgcg acgtggttgg aaaagcatcc cggtctgccg gcggaagtcg
     1141 agatcgccgc tctggaaacg atgtcgctcg tccgaccttc ccagccggag ccgctgacga
     1201 agctggccga gcgcttactg gccaacgcgg atcatacgaa ggaaatcgcc aggcggctga
     1261 ttgccggttc cctggaccga aagctgcaac ccggcgtgat tgaagccctc cgccgtcaca
     1321 gcgaaaggga ttccaccggc gaatgcaaaa cactcctaaa ccaactcctg acgcctgact
     1381 cctgacccct gactcctgac tcctgcccta cgccaccttc agcgcccggt acagatacgg
     1441 attgagcgcc ggatcgttgt acatcttgag ctgccgataa gtcttgtgcc gcttgcggcc
     1501 ggcgaacaaa tcgtcggcca gttgctgggc ggcgagcgac agatcgtcaa gctgcgccag
     1561 ggccaccgcc agcttgcggc cgaccgcttc gcggtgctcg ggcgtcgttt ccggccggtc
     1621 gcgctcctcg gccaggtgat aaatccgcag cgcaaggatc gacagccggt cgatcgtgct
     1681 gccgagcgtt tcggtgttct gcggggcaac ggcgggtgcc agaatgcact ggttggcgag
     1741 cgcctcggtg atccagtcgt ccagccgctc gatccagtcg ttccgctgct ggttgagctg
     1801 gtcgatggcc cgcttgacgg cggcgatctg gctgtcgcct gcctcgcgcg agcgggcctt
     1861 gtcctcttcg tgccagaggc ggaagttgaa cgcgtgctgc tggcaaacga ggccgagcag
     1921 cccctcgtgc ggctggtcca ccggctcctg atgccagcga ctgacgcact cgcgctgcag
     1981 ggtcgtgatt tcgtcgatgc ggatcatcag ggactccgtt cccgtggggt aggcctctgg
     2041 cctgcccgga ttctagcccg attctcgcca ccgcgcaaac gtcaatttcc ccgcccaaag
     2101 cggggaactg tggcagacaa gaatgtctgc cccacgatat gctgaaccta tcagccggag
     2161 aatcgccttg tcccgcactg ccgaaccacg ttccaccagc cgagccccgc gccccagcgg
     2221 cactgtctgg ttctatgtct tgctcctggc cgccggcggc ctggccctgg cggggattgc
     2281 cacgatcaac agcggctcgt cggcccagcg gccggccccg tcgaacctga cgctcgacga
     2341 cattccggtc aacggcgagc gggcgtatgg ctatctgaaa gaaatctgtg cgatcggccc
     2401 gcgcgtttcc ggcaccgagg gaatgcgcct ccagcaggaa taccttcaag cccacttcga
     2461 gaagctcggc ggcaaggtca gccggcaaga gttcgcggcc cgccatccgc agaccggcgc
     2521 ccgcgccctg atggcgaacc tgatcgtcga gtggcatccc gagcggaccg atcgcatttt
     2581 gctctgtgcc cactacgaca cgcgtccctt ccccgaccag gacccggtcg atcccaaggg
     2641 actcttcctc ggcgccaacg acggcgccag cggcacggcc ttgctctgcg agctggctca
     2701 cgacatggcg gacgtgaagg ggaagctggg cgtcgatttc gtcctcttcg acggcgaaga
     2761 attggtctat gacggccagc gcgatccgta cttcctcggc agcgagcact ttagccgcga
     2821 gtatctcgcc aatccgcccg ggcaccgcta caagtacggc gttttgctcg acatggtcgc
     2881 cgattccaac ctggcgattt accgcgaggt gaatagcatg cggacgcgcg aaacgcggca
     2941 gcttgtcaac gacgtgtggg atgtcgcccg cggcctgggc gtgcgggagt t
//
LOCUS       DASUGB010000997         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_805413, whole genome shotgun sequence.
ACCESSION   DASUGB010000997 DASUGB010000000
VERSION     DASUGB010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805413"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFV87_18855"
     CDS             <1..220
                     /locus_tag="VFV87_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105889.1"
                     /translation="VEELLATDKLRPAFLFDRVKTCRVSVEELRRVDPQLATLQNLNR
                     PEDYLAALAAAGFEPDPAILAQLEAPRT"
     gene            complement(126..1004)
                     /locus_tag="VFV87_18860"
     CDS             complement(126..1004)
                     /locus_tag="VFV87_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105890.1"
                     /translation="MDTPEFLVWAIEYTCMVRAQQDGSDPERTERQLRAARAVSDARC
                     EGRVIDGVCVDPPLGFRIDEALQVYGGLQAVENACRGCAANALAQEHSEALAGCYGDV
                     PLPADPRPFHDAIERAIELAGSRQEFRTQPRWYSLWLDSPLSAEVLLDTILILQAAPI
                     ADPRCRAATDELMAGLNAAFNAGCSVHVQLYPPGRVEGAEWTLNSHCPVCRAQWPDKT
                     SHACRVCGFEGHPAPEKRRKARGQRPYIPLERLLGQPQAAEFLVRYEALRAGPKSPDQ
                     AQNQPPPAPPGNPPAG"
     gene            complement(1014..>2055)
                     /locus_tag="VFV87_18865"
     CDS             complement(1014..>2055)
                     /locus_tag="VFV87_18865"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX5105891.1"
                     /translation="DIHWGLLMSNDLRWLEVDSVLAGGKELYKGAHMHPLGEAGRGGG
                     DPGGVDVAADGMVCVAISGVNQVAIGKEGDFSMYRINVGRRPTAVRIAAGGQTAFVAN
                     TFDDSISVIDLVGREVAATIPLGPLPELSLAQQGELLFHNAKLSHDGWMSCQSCHTDG
                     HANGQMNDNFSDRSFGAPKRVLSLLGVKDTLPLAWSGQVTTLERQIHNSVEGTMQREE
                     TLAKPDVQAIAAYVNSLELPPPLDVLRGTQDAASMERGRLVFEKHDCARCHAPPTYTT
                     PEAYDVGLKDSQGATEFNPPSLRGLSHRGPYFHDNSAATLEEVFSKQRHPGDGEFSES
                     EVTDLAAFLRSL"
BASE COUNT          339 a          701 c          663 g          352 t
ORIGIN      
        1 tgtcgaggag ctactcgcga cagacaagct gcggccggcg ttcctattcg atcgcgtgaa
       61 gacctgccgc gtgagcgtgg aggagctgcg gcgcgtcgat ccgcagctcg cgacgctgca
      121 gaacctcaac cggccggagg attacctggc ggcgctggcg gcggctggtt ttgagcctga
      181 tccggcgatt ttggcccagc tcgaagcgcc tcgtacctga ccaggaattc ggccgcttgc
      241 ggctggccga gcagccgctc caacggaatg tagggccgct gtccgcgcgc tttacggcgt
      301 ttttccggcg cgggatgtcc ctcgaatccg cagacgcggc atgcatgcga agtcttgtcc
      361 ggccactgcg ctctgcatac cgggcagtga gaattgagcg tccattcggc cccctcgacg
      421 cgacccggag gatagagctg cacatgcacg ctgcagccgg cattgaacgc ggcattcagt
      481 cccgccatca gctcgtcggt tgccgcacgg cagcgagggt ctgcgattgg cgcagcctgg
      541 agaatcagaa tcgtatccag cagcacttcc gccgacaggg gcgaatcgag ccacaggctg
      601 taccaccgcg gctgcgtgcg gaattcctgg cgggagccgg ccagttcgat cgcccgctcg
      661 atggcgtcat ggaacggacg cggatcggcc ggcagcggca cgtccccgta gcagcccgcc
      721 agcgcctcgg aatgctcctg cgccagagca ttcgccgcac aaccgcggca ggcattctcg
      781 acggcctgca gcccgccata gacctgcagc gcctcgtcaa tccgaaagcc aagcggcgga
      841 tcgacgcaca ctccatcaat cacgcgccct tcgcagcggg cgtcgctcac cgcccgtgcc
      901 gcccgcagtt gccgctccgt ccgctccggg tcgctgccgt cctgctgggc ccgaaccatg
      961 caggtgtact cgatggccca gaccaggaac tcgggcgtgt ccatccaaaa tcgctacaaa
     1021 ctgcgcagga aagcggccag atccgtgact tccgactcag aaaactcacc atcgccggga
     1081 tgccgctgct tgctgaacac ctcttccagc gtggcggcgc tgttgtcgtg aaagtatggc
     1141 ccgcgatgcg agaggccgcg gaggctgggc gggttgaact cggtcgcgcc ttgcgaatct
     1201 ttcagtccca cgtcgtaagc ctcgggtgtg gtataggtcg gcggcgcgtg gcagcgagcg
     1261 cagtcgtgct tctcgaacac cagccggccc cgctccatgc tggcggcgtc ctgcgtgccg
     1321 cggagcacgt ccagcggcgg aggcaactcc agcgaattga cgtaggcggc gatcgcctgt
     1381 acatcgggct tggccagcgt ctcttcgcgc tgcatcgtcc cttcgaccga gttgtgaatc
     1441 tgccgctcca aggtcgtcac ctgaccgctc caggccaagg ggagcgtgtc tttcacaccc
     1501 agcagcgaga gcacgcgctt gggcgcgccg aacgagcggt cgctgaagtt gtcgttcatc
     1561 tggccgttgg cgtggccgtc ggtgtggcag ctctggcagc tcatccagcc gtcgtgcgag
     1621 agcttcgcgt tgtgaaacag cagctcgcct tgctgggcca gcgagagctc cggcaggggc
     1681 ccgaggggga tcgtcgcggc cacttcgcgg cccaccagat cgatgaccga gatcgaatcg
     1741 tcgaacgtgt tggccacgaa tgccgtctgc ccgccggcag cgatgcggac cgcggtcggc
     1801 cggcggccga cgttgatgcg gtacatgctg aaatcgcctt ctttgccgat cgccacctgg
     1861 ttgacgccgg aaatcgccac gcacaccatc ccatcggccg ccacatcgac gccgccggga
     1921 tcgccgcccc ccctgccggc ttcgcccagc gggtgcatgt gggccccctt gtacagctcc
     1981 ttgccgccgg ccaggaccga atcgacctcg agccaacgca ggtcgttcga cattaagagg
     2041 ccccagtgga tgtcg
//
LOCUS       DASUGB010000998        10651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1499269, whole genome shotgun sequence.
ACCESSION   DASUGB010000998 DASUGB010000000
VERSION     DASUGB010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499269"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFV87_18870"
     CDS             complement(<1..239)
                     /locus_tag="VFV87_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105892.1"
                     /translation="MNGKKRNWLVTLAASLALLAGCAPRPAANSSPNAKPAGSGGEAT
                     SVDDSSNAKPAADYTASEILQRLLATYRGAKSYQD"
     gene            complement(284..2266)
                     /locus_tag="VFV87_18875"
     CDS             complement(284..2266)
                     /locus_tag="VFV87_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5105893.1"
                     /translation="MLRAALLPLCGALLLTGVVVAEEKPKPVPILLDTDIGSHVDDAF
                     ALGLALADERVELVGVTTVGEGAEDRAWIVCRLLTHLNRQDIPVAFGRGEQPKGPLDW
                     QIQYRRHPAVVWDRTAKPVKQTAVELMYEKLKTRPGEITIVGIGPLTNVAQLLKEHPD
                     AKSLIKRIVFLGGSLQVGYDGKPPAVAEWTVKQDIEASMKVFHHFAEEVPLVMVPLDA
                     AAGLDLTEEKRRSLFATGTPLASQLHNLWELCETPENALVDSAAVVVAVHEQLAKVRP
                     GRISVGVSGRLSGGILVGGGEPNVQLVTTLDRVGLIDHCFHTLSNHGESAWPAEPKNR
                     SSIVSQGRFPSRVHVFEDYDTDIEKRWWMCGKLETKDVPEGGRRACRAVLTQDFDDRQ
                     GDRGVSYRAVIFNPVPGPPMGPSTRLSFRYKLHGTDTLRVQLFSLSNGYHRYLSVSDL
                     PQDEWQSATVDMTQMRRPDGTGGPLSADERIDDIQFYIDPRAELLIDDVILYDAAAEG
                     EQRPFPKRILFTGWFDTGKQGQEWPGEFEIVPHEKPRTWKFARSITDRETGQPKLVVD
                     LRGPRRLAAVTELTFKYRLTGADSLTMELVDREGNHTVDLSEVKTGEWTEATVPFVVK
                     GAESQDRFASAIRFQPPAGGVLEIDDVLLYEPSAGP"
     gene            complement(2312..3487)
                     /locus_tag="VFV87_18880"
     CDS             complement(2312..3487)
                     /locus_tag="VFV87_18880"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5105894.1"
                     /translation="MPSYLLAARRTPIGKLLGGLSAVPAPQLAAAAIKAALADASLDP
                     ATIDEVVLGNVLSAGIGQAPARQAALAAGIPPSVPALTINKVCGSGLKAVMLADQSIR
                     AGEARVIVAGGMENMSLAPHLLMGVRSGWKFGHQQVLDSMQHDGLWCSTENQGMGSLA
                     DYIAAKCHISREDQDAFAAESHRRAAAARVAGLFEAEIVPVAVKAGKTETIVHQDEGP
                     RPGCTIADLARLRPAFAADGTATAGNASQISDGAAALVVADEATAREHHSPLKARIVA
                     SATHAVAPKELFIAPVGAIEKVLAKAKLKIGDIDLFELNEAFASQCLACMRPLGIDHD
                     KLNVHGGAIALGHPIGASGARVLVTLLHALAARNLHYGLAALCLGGGEAVAMVVERV"
     gene            3594..4568
                     /locus_tag="VFV87_18885"
     CDS             3594..4568
                     /locus_tag="VFV87_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5105895.1"
                     /translation="MINPELRHLCATTVYVSPVALGCWPISGMTSLDVNEADSLATLH
                     AAIEAGVNFFDTAYAYGAAGESERLIARAVAGRRDKVVIATKGGIEWDSAGQRVIDGR
                     PQTLRRQCEESLRRLATDRVELLYLHAPDPQTPLAESAGELKRLMEEGKTLAIGVSNF
                     SLPQFKEFHAACPISAFQPPYSMLQRDIERDTLPWCRERSISVCVYWPLLKGLLAGKL
                     PRDHTFQPGDGRAKYPMFQGQEWQRNQDLLDELRRIAAEVEKTVAQLVINWTIHQPGI
                     TAALCGAKRPEQIRETADAMGWRLSPEHSARIDAALARRGQPVTKGAV"
     gene            4568..5905
                     /locus_tag="VFV87_18890"
     CDS             4568..5905
                     /locus_tag="VFV87_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5105896.1"
                     /translation="MPHELPPGPGSPAWWQLVRYSLWPLGFLERGRRRFGETFTTRMA
                     GYGKIVILSNPEAVKGVFRGDPRVLHSGEGNEFLSTSVGKHSVLVLDDEPHERQRRVL
                     LPPLKGERMRAFFAAIQEATLETIGSWKVGNRVKLEEPMRRITARVILQAVLGWPVDE
                     RLKEMEAKVHHLLSYTRSRWSLILMKLFSPQRFKPSQKVPFYRALSQLDAGVYAAMFR
                     ARQEPAGSRPECVLTDLLSATHADGTPLADEEIRDAVVTLLFAGHETTSVALAWAFAE
                     IVPHENVVGEIQHELHRVCGEALPTAEQMPQLEYLDAAIREVLRLRTVLPFVVRKTKA
                     PFVAGGREYPEGVLLAPCSHLVHQRPDLFPDPGKFRPERFLERKFTPYEWFPFGGGNR
                     VCLGMAFALYEMKVVLATVLATCRLSRPRPEGRYVRRGLTLAPSDGAWVVREK"
     gene            6068..6439
                     /locus_tag="VFV87_18895"
     CDS             6068..6439
                     /locus_tag="VFV87_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX5105897.1"
                     /translation="MLYGTIKNVAPNRGFGFIEPSGSGLDVYFHATVIGDDVFQRLRP
                     GQAVKYELVKLTEAERERRRELKEKPKPKAGKIELIDKLPGGILPRPTQDIAPRHHPK
                     ARQRKATWKRKIDLSEKNKKS"
     gene            6534..7076
                     /locus_tag="VFV87_18900"
     CDS             6534..7076
                     /locus_tag="VFV87_18900"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5105898.1"
                     /translation="MGADDSIPEFLGRTPEQWLARLQSAQPQERTEAAWAIAELAVRD
                     PLLAVRLAHNDDSIVRYWGVQGLQRSALATGESNSTRKMALRAVVPLLADKTPAVRIV
                     AAETLGLLGDPDRALPVLVAAMSHPQDAVRIQAIAGLERLGEAARPAEKTLQAATTDT
                     SEYVKRISTRALQKLETTRK"
     gene            7185..9422
                     /locus_tag="VFV87_18905"
     CDS             7185..9422
                     /locus_tag="VFV87_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX5105899.1"
                     /translation="MLRYLSLAAFCFVSGLASAADESAPQLSVKDLATKVKNSIVVIS
                     HGGREGGQQGLGTGFVIDKAGLIATNLHVIGEARPISVQTADGKSLAVKAVHASDRAL
                     DLAIIQVEATDLPALELGNAAKLAQGEPVVMMGNPQGLKHSVVSGVVSGTREIDGRDM
                     LQLAIPVEPGNSGGPVVDMQGRVLGIMTMKSLVTENLGFAVSVSDLVPLVEKPNPVPL
                     DRWLTIGQIDRSQWEPLFGARWQQRAGKILVDGLGSGFGGRSLLLASEMPPELPYEVA
                     VNVKLGDESGAAGLVFHADGRDQHYGFYPTNGKLRLSRFEGPDVFSWQVLVEKPSEHY
                     KPGDWNRIKVRVEKDKLSCFVNDELVIESTDDAFSRGRVGLAKFRNTAAEFRRFAVAK
                     ELPSNRPDEAALAKLAEQIGSLPPLAETGDATVASLAHADGRDARGALLAKAAELEAR
                     AGELKRLAADVRTATVVAEFEALAGPKVEKIDLLRSALTVARLDEEDLEVEAYARHVE
                     RMADEIQKKLPANATEGQKVSALNDYLFKDNGFHGSRTDYYHRANSYLSRVIDDREGL
                     PITLSVLYLELGSRLGLKIEGVGLPAHFVVRHVPAEGDSQLIDVFEGGEFLSREDAEK
                     RIRTLTGEPPLESHFAAVTERQILMRILTNLIGNAQQPQKGPDREALIRYESAMLALD
                     PSLVRDRGLRAVCRWETGRRDAAVADLQFILDAKPEGIDLQELRNMQEYFRTTRPPQL
                     QSSSR"
     gene            9585..>10651
                     /locus_tag="VFV87_18910"
     CDS             9585..>10651
                     /locus_tag="VFV87_18910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105900.1"
                     /translation="MAGSKRREKLEKEKRRRKKLEARRAAQPAHRSIAEQASRLIEDG
                     EWGEAREILEAYDRAHAPRSDVLKLLLDVCHEQEDMGGICSAAGRLLKIEPDNPPLHL
                     MFASACMCEARPASALLGFRRYVERWPDDPLAAGVRDSIAELEPLVEELLREAPFPAK
                     QRLELAALHEETLAALGSGNYERCIEAGERLLARCPAFINVRNNLSQAYFLLGRTEQA
                     LAASRAVLRQQPNNPHALANLARHLLLLGQNEEAQAACQRLREVETDNPDIWSKKAET
                     FAFFGDDEAVLETFAAAEQAGHLREKSPDVALLYHLAAVALARQGNHRQARKHWQSAL
                     QLAPGFELAEENLADSRKPVG"
BASE COUNT         1924 a         3368 c         3490 g         1869 t
ORIGIN      
        1 tggtcctggt agctctttgc gccgcggtag gttgccagca ggcgctgcag aatctcgctg
       61 gccgtgtaat cggctgcggg ctttgcgttg gacgaatcgt ccaccgaggt ggcttcgccg
      121 cccgatcctg cgggcttcgc attcggcgag gaattggccg ccggccgcgg tgcgcagccc
      181 gcgagcagag ccaggcttgc cgccaacgtg accaaccagt ttcgcttctt gccgttcatt
      241 cttgcattgt gccagtagcg gtgggtttca caaaggcgag ttgctacggc cccgcgctgg
      301 gctcgtaaag caacacatcg tcaatctcca gcacgccgcc ggccggcggc tggaagcgga
      361 tggcggatgc aaacctgtcc tggctttcag cgcctttgac cacaaacgga acggtagctt
      421 cggtccattc gcccgtcttg acctcggaca gatcgaccgt gtggttgcct tcgcgatcga
      481 ccaattccat cgtcagcgag tccgcgcccg tcagccgata cttgaacgtc agctccgtca
      541 ccgccgccag ccgccgcggg ccgcggagat cgacgacgag cttcggctgg cccgtttcgc
      601 ggtcagtgat tgagcgggcg aatttccagg ttcgcggctt ctcgtgcggg acgatttcga
      661 attcgcccgg ccattcctgg ccttgcttgc ccgtgtcgaa ccagccggtg aagaggattc
      721 gcttggggaa ggggcgctgt tcgccctcgg ctgcggcgtc gtagaggatc acgtcgtcga
      781 tcaggagttc tgcccgcggg tcgatgtaga actggatgtc gtcgatccgc tcatcggccg
      841 agagcggccc gcccgtgcca tcgggcctcc gcatctgggt catatcgact gtggccgatt
      901 gccactcgtc ctgcggcaag tccgagacgc tcaggtagcg gtgatagccg ttcgacagac
      961 tgaagagctg cacgcgcagc gtgtcggttc cgtgcagctt gtagcggaac gacagccgcg
     1021 tgctgggccc catcggcggg ccgggcactg ggttgaagat cacggcccga tagctcacgc
     1081 cgcggtcccc ctgccggtcg tcaaaatcct gcgtgagcac cgcccggcac gcccggcggc
     1141 ccccttccgg cacgtccttc gtctccagct tgccgcacat ccaccagcgt ttctcgatgt
     1201 ccgtgtcgta gtcctcgaac acgtgtacgc ggctgggaaa gcggccttgc gaaacgatcg
     1261 aagacctgtt cttcggttcg gcgggccaag cggattcgcc gtggttggac agcgtgtgaa
     1321 agcagtgatc gataagcccg acccgatcga gagtggtcac cagctgcacg tttggctcgc
     1381 cgccccccac gagtatgccc ccgctgagcc ggccgctcac acctacggat atccgaccag
     1441 ggcgaacctt tgctagctgc tcgtggaccg cgacgactac ggcagccgaa tcgacgagtg
     1501 cgttttcagg tgtctcgcag agttcccaga gattgtgcag ttgcgaggcg agcggcgtcc
     1561 ctgttgcaaa tagactccgg cgtttctcct cggttaaatc caggccagcc gcggcgtcga
     1621 gcggcaccat gaccagcggc acttcttcgg cgaaatgatg gaaaaccttc atggacgctt
     1681 cgatgtcttg ctttacagtc cattccgcca ccgctggcgg cttgccgtcg tagccgactt
     1741 ggagcgagcc gccgagaaag acgatccgct tgatgagcga cttggcgtcg ggatgttcct
     1801 tgagtagctg cgcgacgttc gtcagcgggc cgatgccgac gatcgtgatc tcccccggcc
     1861 gcgtcttgag cttttcatac atcaactcga ccgccgtctg cttcaccggc ttggcagtcc
     1921 ggtcccagac gacagccgga tggcggcggt attgaatctg ccagtcgagc ggccccttcg
     1981 gctgctcgcc gcggccaaag gcgacgggga tgtcctgtcg attcaagtgc gtcagcagcc
     2041 ggcagacgat ccacgcccgg tcctccgccc cttcgccgac ggtcgtcacg cccaccaact
     2101 ccacccgctc gtccgcgagc gccagtccca gtgcgaacgc gtcatcgaca tgcgagccga
     2161 tgtccgtgtc gagcaggatg ggaaccggct ttggcttctc ttctgcaacc acgacgcccg
     2221 tgagaaggag cgcaccacat agcggcaaca aagcggctct gagcatcgtt cacctccgct
     2281 ggcgattggt tagcaagcat gctcgccaac tctacacccg ctcgacaacc atcgccaccg
     2341 cttcgccgcc ccccaggcag agagccgcca gaccgtagtg caagttgcgg gcggccagcg
     2401 cgtgcagcag tgtgacgagc acccgcgcgc cgctggcgcc gatcggatga ccgagagcga
     2461 tcgccccgcc gtggacgttg agcttgtcgt gatcgatgcc cagcggccgc atgcacgcca
     2521 ggcactgcga ggcaaatgct tcgttcagct cgaagagatc gatgtcgccg atcttcagct
     2581 tcgccttcgc cagcaccttt tcgatcgcac cgaccggagc gatgaacaac tccttgggag
     2641 cgactgcgtg cgtcgcgctg gcaacgatcc gcgccttcag cggcgagtgg tgctcacggg
     2701 cggttgcttc atcggcgacc accagtgcag ccgcgccgtc gctgatttgc gaagcattgc
     2761 cggccgttgc cgtgccgtcc gccgcgaatg ccgggcgcaa tctggccaga tcggcgatgg
     2821 tgcagccggg ccgcggacct tcgtcttgat ggacgatcgt ctccgttttg ccggctttca
     2881 ccgccacagg cacgatttca gcctcaaaca ggccggccac gcgggccgcc gccgcccgcc
     2941 ggtggctttc cgcggcgaat gcgtcctggt cctcgcggct gatgtggcac ttggcggcga
     3001 tatagtcggc cagcgacccc atgccctggt tttcggtcga gcaccacaga ccgtcgtgct
     3061 gcatcgaatc gagcacctgc tggtggccga atttccagcc ggagcggacg cccattagca
     3121 aatgcggcgc caggctcatg ttttccatac caccggcgac gatgacccgc gcctcgcctg
     3181 ctcggataga ctgatcggcg agcatcaccg ctttcagccc gctgccgcag actttgttga
     3241 tcgtgagcgc gggcacgcta ggcggaattc cggccgccag cgccgcctgc cgcgcgggcg
     3301 cctggccaat gcctgcagaa agcacgttgc cgaggacgac ctcatcgatc gtcgctggat
     3361 cgaggctcgc gtcggccagc gccgccttaa tcgccgccgc tgcgagctgc ggggcgggga
     3421 cggccgaaag cccgccgagc agcttgccga tcggcgtgcg gcgggcagcg aggaggtagc
     3481 tgggcatggg caggttatag atcgggggct atagatgggc cggcgaaatt gcaaaataca
     3541 aggcagtaca attctacggc ggagattcac gctacgctga ctcgcaacgt ccgatgatca
     3601 atcccgaatt gcggcatctg tgtgcgacga ccgtttatgt atcgcccgtg gccctgggct
     3661 gctggcccat ttcgggcatg accagcctgg acgtgaatga ggcggacagt ctggcgacgc
     3721 tccacgccgc gatcgaagcc ggcgtcaact tcttcgacac ggcctatgcc tacggcgcag
     3781 cgggcgagag cgagcggctg attgcccgcg cggtcgccgg ccggcgcgat aaggtcgtca
     3841 ttgcaaccaa aggaggcatc gagtgggatt ccgctggcca gcgggtgatc gacggccgcc
     3901 cgcagacgct ccgccgccaa tgcgaagaaa gcctgcggcg gctcgcaacc gatcgcgtcg
     3961 aactgctgta tctgcatgct ccagatccgc agacaccact ggccgaatcg gcgggcgaat
     4021 tgaagcgcct gatggaggaa ggcaagacgc tggcaatcgg cgtctcgaac ttcagcctcc
     4081 cccagttcaa agaattccac gccgcctgcc cgatctccgc gttccagccg ccatacagta
     4141 tgctgcagcg ggacattgag cgggacaccc tcccgtggtg ccgcgaacga tccatttcgg
     4201 tttgcgtcta ttggccgctg ctcaagggcc tcctcgccgg caagctgccg cgcgaccata
     4261 ccttccagcc gggcgacggc cgggcgaaat atccgatgtt ccagggccag gaatggcagc
     4321 gcaatcagga cctgctggac gaattgcgac gcatcgcggc ggaagtagag aagaccgtcg
     4381 cccagcttgt catcaactgg acaatccacc agccgggaat taccgcggcc ctctgcggcg
     4441 ccaagcgccc cgagcaaatc cgcgaaaccg ccgacgcgat gggctggcgg ctttcgcccg
     4501 agcactcggc gcgcattgac gccgccctcg cccgccgtgg ccagcccgtg acgaaggggg
     4561 cggtctaatg ccgcacgagc ttcctcccgg ccctggttct cctgcctggt ggcagctcgt
     4621 gcggtattcg ctttggccac tgggctttct ggagcgcggg cgaaggcggt ttggcgagac
     4681 gttcacgacg cgaatggccg gctatggcaa aatcgtgatc ctctcgaacc ccgaggcggt
     4741 gaagggcgtg tttcgcgggg atccgcgcgt gctgcactcg ggtgagggga acgaattcct
     4801 cagtacgtcg gtcggcaagc attcggtgct ggttctcgac gatgagccgc acgagcgtca
     4861 gcggcgcgtg ctcctgccgc cgctcaaagg cgagcggatg cgggcgttct tcgccgcgat
     4921 acaagaggcg acgctggaaa cgattggctc gtggaaggtc ggcaacaggg tcaagctcga
     4981 agagccgatg cggcgaatca ctgcccgcgt aatcttgcag gcggtcctcg gatggccggt
     5041 cgatgagcgg ctgaaggaga tggaggccaa ggtccaccac ttgctgtcgt acacccgcag
     5101 ccgctggtcg ctgattttga tgaagctctt ttcgccacag cggttcaagc cctcgcagaa
     5161 agtgccgttt tatcgggcct tgagccagct cgatgcaggc gtctatgccg cgatgtttcg
     5221 cgcccgccag gaaccggccg gttcgcggcc cgagtgtgtg ctgaccgacc tcctttccgc
     5281 cacgcacgcc gatggcacgc cgctggcgga cgaggagatt cgcgacgccg tcgtgacgct
     5341 cctctttgcg gggcacgaaa cgacctcggt cgccctcgcc tgggcgtttg ccgagatcgt
     5401 gccgcacgag aacgtcgtcg gcgaaattca gcacgagctg caccgcgtct gcggcgaagc
     5461 cctgccgacc gccgagcaga tgccacagct cgagtatctc gacgccgcca tccgcgaagt
     5521 gctgcggctg cggactgttc ttccgtttgt cgtacgcaaa acgaaggcgc cgtttgtggc
     5581 aggcggacga gagtatcccg agggggtgct gctcgcgccc tgctcgcacc tggtgcatca
     5641 gcggccggac ctgtttcccg acccggggaa attccgcccc gagcgtttcc tggagcgcaa
     5701 gttcaccccc tacgagtggt tcccctttgg cggcggcaac cgggtctgcc tgggcatggc
     5761 gtttgcgctg tacgagatga aggttgtcct ggcgaccgtg ctggccacgt gccgcctctc
     5821 gcgcccccgt cccgaagggc gctacgtccg ccgcggcttg acgctcgcgc ccagcgatgg
     5881 cgcgtgggtg gtccgagaaa agtaggttcc gacggccgcg gcggtaccgg aagtacctgc
     5941 caacttcgac cgccacccca ggtcccgccc aggcggtcgg gacctacaag agcggcgcaa
     6001 agtgaagtaa aagaggggtc ggcgagcggg gggcgtcagc cccctgagaa ttctgagaaa
     6061 tcgaaacatg ctctacggca cgatcaaaaa cgtcgccccc aatcgcggct ttgggttcat
     6121 tgagcccagc ggcagcgggc tggatgtcta tttccacgcg acggtcatcg gcgacgacgt
     6181 cttccagcgg ctgcggccgg ggcaggcggt gaagtacgag ctcgtcaagc tcaccgaggc
     6241 cgaacgcgag aggcgccgcg agctgaagga aaagcccaag cccaaagccg gcaagatcga
     6301 gctgattgac aagctccccg gcggcattct tccgcgaccg acccaagaca tcgccccgcg
     6361 gcaccatccc aaagcccggc agcggaaagc gacgtggaag cgcaagatcg acttgtcgga
     6421 gaagaacaaa aagtcgtgag gtcagcatga gtgctatgtg tcgtacgccg ggatggctga
     6481 gtactcagta cttcatactc agtactttgc ttttgatcgg gagccctgtc tgtatggggg
     6541 ccgacgattc cattcccgag tttcttggcc gcacgcccga gcaatggctc gcccgactgc
     6601 aatccgcgca gccacaagag cggactgaag ccgcttgggc catcgcggag ctcgcggtgc
     6661 gcgatccgct actcgctgta cgactcgccc acaatgatga ctcgatcgtg cgctactggg
     6721 gcgtgcaggg cttgcagcga tccgcgctcg ctaccggcga gtcgaactca acgcgaaaaa
     6781 tggccctccg tgctgttgtg ccgcttctgg ccgacaagac gccagccgtc cgcatcgtcg
     6841 cagcggaaac gctcgggctc ttgggcgatc ccgatcgggc gctgcccgtg ctcgtcgcag
     6901 ccatgagcca cccgcaagac gccgtccgca ttcaggccat cgccgggctg gaaagactcg
     6961 gcgaagcggc ccgccccgcc gaaaagacgc tgcaagcggc aaccaccgac acgagcgagt
     7021 acgtcaagcg catctcgacg cgggcgctgc agaagcttga gacgacaaga aaataggaca
     7081 ttaggtcgca aagacgcggg aacttggatg aatcgtgccg ctgccggtca tagaatcgag
     7141 attgcctttt gttcgcaatt tgttcctcgc ccggctgagc cttcatgttg cgttatcttt
     7201 cgcttgcggc tttctgtttt gtctctggtt tggccagcgc ggctgatgag tccgcgccgc
     7261 agttgtcggt gaaagacctc gcgaccaagg tcaaaaattc gatcgtcgtc atcagccacg
     7321 gcgggcgtga aggcggccag caggggctgg gaacggggtt tgtgatcgac aaggcggggc
     7381 tgattgcgac gaatctgcac gtgatcggcg aggcgcggcc gatcagcgtg caaacggcgg
     7441 acggcaaatc gctggcggtg aaggcggtgc atgcttcgga ccgggcgctc gacctggcga
     7501 tcattcaggt cgaggcaacg gatttgccgg cactggagtt ggggaacgcg gccaaactcg
     7561 cgcagggcga gccggtcgtg atgatgggca acccgcaagg gctcaaacac agcgtggtca
     7621 gcggcgtcgt cagcgggacg cgcgaaatcg acggccgcga tatgctgcag ctcgcgattc
     7681 cggtcgagcc gggaaacagc ggcgggccgg tcgtcgatat gcaaggccga gtgctgggca
     7741 tcatgacgat gaaatcgctg gtcacggaaa atctggggtt tgcggtctcg gtcagcgatc
     7801 tggtgccgct cgtcgaaaag cccaatcccg tcccgctcga tcgctggctg acgatcgggc
     7861 agatcgatcg ctcgcagtgg gagccgctgt ttggcgcccg ctggcagcag cgcgccggga
     7921 aaatcctcgt cgatggcctc ggctcggggt tcggcggccg gtcgctcctt ttggccagcg
     7981 aaatgccgcc ggaattgccg tatgaagtgg ccgtcaacgt caagctgggc gacgaatcgg
     8041 gcgctgcggg gctggtgttc catgccgatg gccgcgacca gcattacggc ttttatccca
     8101 caaatggcaa gctgcgcctg agccggtttg aagggccgga cgtgtttagc tggcaggtgc
     8161 tcgtcgagaa gccgtcggag cattacaagc cgggcgattg gaaccgcatc aaggtccgcg
     8221 tggagaagga caagctgagc tgcttcgtga atgacgagct ggtaatcgaa tcgaccgacg
     8281 acgccttcag ccgcggccgc gtcggtctgg caaaattccg taacacggca gccgagtttc
     8341 gccggttcgc ggtggcgaag gaattgccga gcaaccgccc cgacgaggct gctttggcga
     8401 agctggccga gcagatcggc agcttgccgc ctctagccga gacgggcgat gcgaccgttg
     8461 cctcgctggc gcatgctgac ggacgcgacg cccgcggggc gctgctcgcc aaagcggccg
     8521 agctcgaggc tcgcgccggc gaactgaaac gcctggcggc tgatgttcgc acggccaccg
     8581 tcgtcgcgga attcgaggca ctggccggtc ccaaggtgga gaagatcgac ctgctgcgct
     8641 cggcattgac tgtcgcccgg ctggacgaag aagacctgga ggttgaggcg tacgccaggc
     8701 acgtcgagcg gatggcggac gaaatccaga agaagctgcc ggccaatgcc acggaaggcc
     8761 agaaggtgag cgccctgaac gactatctgt tcaaggacaa cgggtttcac ggcagccgga
     8821 ccgattatta ccaccgggcc aacagctact tgagccgcgt gattgacgat cgcgaagggc
     8881 tgccgatcac gctttcggtg ctgtacttgg aactcggttc gcggctggga ctgaagattg
     8941 agggggtcgg cttgccggcg cactttgtcg tccgccacgt accggcggag ggcgattcac
     9001 agctcatcga cgtcttcgaa gggggcgaat ttctgtcgcg cgaggacgcg gaaaagagaa
     9061 tccgaacact gacgggcgag ccgccgctgg aatcgcactt cgccgcggtc acggagcggc
     9121 aaatcttgat gcggatcctg accaacctga tcggcaacgc ccagcagccg cagaaaggcc
     9181 ccgatcgcga ggccctgatc cgctacgaga gcgcgatgct ggcgctcgat ccgtcgctgg
     9241 tgcgcgatcg cggcctccgc gccgtctgcc ggtgggaaac gggccggcgc gatgcggctg
     9301 ttgccgacct gcagttcatt ctcgatgcca agcccgaggg aatcgacctg caagagctgc
     9361 ggaacatgca ggaatacttc cgcacgaccc gcccgccgca gctccagtca tcttcgcgat
     9421 aggagcagag agcgaccccg cggcggcaag ttcggattga cggcgcacgg catcccggat
     9481 tgaataatcg ctgtcagtct gcacaccgcg atcgggaatg acgcaagcgt cgagtgcgcg
     9541 ctcccaggtt ttggacggaa tcgcgatcca cggaggtagg cacggtggct ggaagcaagc
     9601 gacgcgaaaa gttggaaaaa gaaaaacggc ggcggaagaa gttggaggcg cggcgtgccg
     9661 cccagccagc gcatcgaagc atcgctgagc aggcgtcgcg gctgatcgag gacggggaat
     9721 ggggggaggc tcgggagatt ctcgaagcct acgaccgtgc gcacgcgccg cgatcggacg
     9781 tcctcaaact gctgctcgac gtctgtcacg agcaagagga tatgggaggc atctgcagcg
     9841 cggccgggcg gctgctcaag atcgaacccg acaacccgcc gctgcacctg atgtttgcca
     9901 gcgcctgcat gtgcgaagcg cggcccgcct ccgcgctcct ggggtttcgc cgctacgtcg
     9961 aacgctggcc cgacgatccg ctggcggcgg gcgtgcgcga ttccatcgcc gagctggagc
    10021 cgttggtcga ggagctcctg cgagaagcgc cgtttcctgc caaacagcgg ctggaactgg
    10081 cggcgctgca cgaagagaca ctggcggctt tgggctcggg aaattacgaa cgatgcatcg
    10141 aagcggggga gcggctgctc gcgcggtgtc ccgcgttcat caacgtgcgg aacaacctta
    10201 gccaggccta ttttctgttg ggacggaccg aacaagccct ggcggcgtcg cgggcggtgc
    10261 tccggcagca gcccaacaac cctcacgccc tggccaatct ggcgcgtcac ctcctgttgc
    10321 tggggcagaa cgaggaggcg caggcagcgt gccagcggct gcgtgaagtt gaaaccgata
    10381 atcccgacat ctggtccaag aaagctgaga cgttcgcctt cttcggcgac gacgaggcgg
    10441 tgcttgaaac ctttgcggca gcggagcagg caggacacct gcgcgaaaag tcaccggatg
    10501 tagcgctcct ctatcacctg gcagccgttg ctctggcgcg ccagggcaac catcggcaag
    10561 cgcgaaagca ttggcagtca gcacttcagc ttgcgcccgg gtttgagttg gcggaggaga
    10621 atctggccga ctccaggaag cccgtgggcg a
//
LOCUS       DASUGB010000999         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_109924, whole genome shotgun sequence.
ACCESSION   DASUGB010000999 DASUGB010000000
VERSION     DASUGB010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109924"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2295
                     /locus_tag="VFV87_18915"
     CDS             <1..2295
                     /locus_tag="VFV87_18915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105901.1"
                     /translation="ADVGRLFALETLPPAGVGRTIEISYPDFRDYRDSSQSLAGAIAF
                     RERALSLGDGIQAERVWAQFVSPNFFDVLGVMPVTGRFFQTEEQEDAPGKYPVAVISE
                     RVWQRRFHTDPEIIGKAVRLNGRDLTVIGVAPAEFLGTIVGLSYDFWLPVSMESLLSG
                     GGSGLESRNARRLHVLARFRPGVSLPQARAEMQTIAQRLARSYPQSNTGIGTTLVPIS
                     QANTGAQELMAPVLKTLLGAGAVLLLIVCANVANLMLLRATARQKELDIRLALGASGS
                     WVIGQLLLESLLLAGLGGAAGAALAGRMAGWLQFFTPATHLPVTLNFPLDATVLAFNL
                     TISLVAGLLCGLIPALQIVRRGKFGSLRDSGRGAIGDARAHRLRGGLVISEVALALVV
                     LIGASLFFKSVQYAEHTDPGFDPSRMLLAGLDLSEQGYSAESGKLFMRQLRDRLAVLP
                     GVQAVSFNQTPPLGFERPNSTPVQIEGYLPREGEDMRTSWDQVWPGYFDLMRIGLIEG
                     RDFTDSDDGQALPVVIVSEAFASRFFAGQEPVGRKLRLGGGGERTVIAVAKDINYHSL
                     GESPQPYFYLPMQQAWQPNTAFALSVRTEGPPEQILPALQRELKSVDPRANLTAVFAF
                     SDFIAASWFTQKIAATLLGVLGTLALLLAALGLFSVMAYTVRQRTQEIGIRMALGAQV
                     MDVFRPVLGQALRLTMVGVGIGLVVSFALTRLVASQLLGVSATDPLTFVGVSCLLCAV
                     ALAASYLPARRATRVDPLVALRAE"
BASE COUNT          404 a          691 c          791 g          509 t
ORIGIN      
        1 gcggatgttg ggcgtctctt cgcactcgag accctcccgc cagccggggt cggcagaacc
       61 atcgagattt cgtatcctga cttccgggat tatcgcgact catcgcaatc gcttgccggc
      121 gcaatcgcat tccgcgagcg ggctttgagt ctcggagatg gaatccaagc ggaacgcgtc
      181 tgggcacagt tcgtgtctcc gaatttcttc gacgtgctcg gcgtcatgcc ggtgacgggc
      241 cggttcttcc agaccgagga gcaggaagac gcgccgggca agtatccggt ggcggtcatc
      301 agcgagcgcg tttggcaacg gcgtttccac acggaccccg agatcatcgg gaaggcggtt
      361 cgcctgaacg gccgggacct gacggtgatc ggcgtcgccc cggcggaatt cctgggaact
      421 atcgttggcc tttcatacga cttctggctg ccggtttcga tggagtcgtt gctgagcggc
      481 ggcggcagcg ggcttgaaag tcgcaacgct cgacggttgc atgtcttggc tcggtttagg
      541 cctggcgtgt ccctgccgca ggcccgggca gaaatgcaga ccattgcgca gcgtttggca
      601 cggagctatc cgcagagcaa cacaggtatc ggcacgacgc tggtgccgat ctctcaagct
      661 aacactgggg cgcaagaatt gatggcgcct gtgctcaaga ccctgctcgg ggctggggcc
      721 gttctcctgc tgatcgtctg cgccaacgtg gcgaatctga tgttgcttcg tgccacggct
      781 cggcagaagg agctcgatat ccggctggcg ctgggcgcct cgggctcgtg ggtgatcggt
      841 cagctgctgc tcgagagcct attgctcgct ggccttgggg gcgcagcggg cgccgcattg
      901 gccgggcgga tggcggggtg gctgcagttc ttcactcccg cgacgcacct tcccgtgact
      961 ctcaatttcc cgctggacgc tacggtacta gcattcaact tgacgatttc gctcgtggcg
     1021 ggacttctgt gtggcctgat tccggcgctg cagatcgttc ggcgcggcaa gttcgggagc
     1081 ttgcgggata gtggacgcgg agccatagga gatgcacgcg cgcatcgctt gcggggaggg
     1141 ctggtcattt ccgaggtcgc cctggcgctg gtggtgttga tcggcgcgag cttgtttttc
     1201 aagagtgtgc agtacgccga gcacactgac ccgggattcg atccctcacg catgcttctg
     1261 gccgggttgg atctatcgga gcaaggatat agcgccgagt cggggaagct cttcatgcgg
     1321 caactgcgcg atcgtctcgc agtcttgcct ggagtgcagg ccgtgagttt caaccagacc
     1381 ccgccactcg gcttcgagcg ccccaattca acgccggtgc agatcgaggg gtatctgccc
     1441 cgcgagggcg aagacatgcg gacctcctgg gatcaggtgt ggcctgggta cttcgacctc
     1501 atgcggatcg ggctcattga aggccgggat ttcaccgata gtgacgatgg ccaagcgctg
     1561 ccggtggtca ttgtcagcga ggctttcgcg agtcgatttt tcgcaggaca agaaccggtt
     1621 gggcgcaagc tgcggcttgg aggaggaggc gagcgcacgg tgattgcggt cgccaaggac
     1681 atcaactacc acagtctcgg tgagagtccg cagccgtact tctatttgcc gatgcaacaa
     1741 gcctggcagc ccaacaccgc ctttgcgctg tctgtgcgaa cagagggtcc gccagagcag
     1801 atcttgcccg ccctgcagcg tgaattgaaa tccgtcgacc ctagggcgaa tctcactgcg
     1861 gtatttgcgt tcagtgattt catcgctgcc tcctggttta cacagaagat tgcggcaact
     1921 ttattggggg tcttgggcac tctggcacta ctgctcgccg cgctgggcct tttcagcgta
     1981 atggcctaca ccgtgaggca gcgcacccag gaaatcggca tccggatggc cctcggcgcc
     2041 caggtgatgg atgtgtttcg gcccgtcctt ggtcaggcgc tcaggctgac gatggtggga
     2101 gtcggtattg gactcgtagt ctccttcgcg ctcacacggc tcgtagcgag ccaattgctc
     2161 ggcgtaagtg cgacggaccc gctcacgttc gtcggcgtgt cctgcttgct ctgcgcagtc
     2221 gcgctggcag cgagctatct accggctcga cgcgcaactc gagtcgatcc attggtcgcg
     2281 ctgcgagcgg agtagaaggt ttccctgaat cgacgaacca ctgcagcgcg aatttgaacg
     2341 agacgaagta cgggaaatgc gtgccgtgta ttggttgaag cggcctgagc cgagc
//
LOCUS       DASUGB010001000         6977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_805576, whole genome shotgun sequence.
ACCESSION   DASUGB010001000 DASUGB010000000
VERSION     DASUGB010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805576"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFV87_18920"
     CDS             complement(<1..1452)
                     /locus_tag="VFV87_18920"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5105902.1"
                     /translation="MTSSSHRVSLTIAAILALALLPGCHDVGGNRLRDEAQRQADFKN
                     RKIARPKLPDQPKQQDAQDELAVIARAYQATRSGSINIGRAEDVAVKKVGLAIKASRE
                     IGPTLVVWIVDRTPSSQKIVAQATSAVKNYYDSQDVRQWSLSEDDPLLTAVIAYDETP
                     ELLLDPPTGDWQKVKAAWDGIQPSTAGRENTFAAIKQALEKYLTFRTDQRRELLLLVI
                     TDEAGDDPQLAEELAESTRRQAIPIYVIGSPAPWGQVNPFAADPKAIDTTKMDDSAPV
                     HGPESLFSERVDIQGWAASYGFDSGQRPDFALIDSGFGPFALERLCRASRGQYFAIRP
                     EAGSSAYSYRGETYQYWPPGNELRFTTESQSRYAPDYVSEAEYRQLLAENKARLALHE
                     AARLPKVVVEDYPSLSFPKGTEAQNARNLNSAQQFAAKYAPAVDRLYNVLAPGEGDRE
                     KLTSPRWQAEFDLAMGRVLALKARLDGYNSMLAA"
     gene            1627..3024
                     /locus_tag="VFV87_18925"
     CDS             1627..3024
                     /locus_tag="VFV87_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105903.1"
                     /translation="MRRILMTAMIGCLALAWAAPARACGSYLRMQVLQALADGANAAT
                     IEELRAAGPAALDELFQLRDQMTKSLGEKTSQPTPTADEVIQIERLQQRIQRLDEMID
                     QVGMQRYCSRSRLYWYTDFDKAVTAARESGKPILSLRMLGNLNEDFSCANSRFFRTTL
                     YANAEISKTLRENFVLHWKSVRPVPRVTIDFGDGRKLERTVTGNSAHYILTPDGEVVD
                     CLPGLYGPKAFLRKINEGLSMARQVAALSPSERGERLAAFHAKELADLQSAFGRDLIL
                     VSAANDRALTAAPQPSQQTRAAPPANVPTSLARPKARIETRLVAAAIPTAADSLSLDD
                     DAVWQRIAALHAEDAQLDSASRELIKSENPTAAVAGRLAMTKALVENPLVRLVRSLQE
                     SIGLDTVKNEYRFHRQIHGWLADGNYRPGIDELNERVYAELFLTPSSDPWLGLAPPDA
                     YTALPNGGVVANRGP"
     gene            3165..3866
                     /locus_tag="VFV87_18930"
     CDS             3165..3866
                     /locus_tag="VFV87_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5105904.1"
                     /translation="MPKTILTVEDDAAIRRGIVDALKYVGYGVLEAGNGNLGHEMAVQ
                     QAYDLLLLDLVLPGRGGLEILRAVRTTRPTQPVIILTARGEENDRVEGLKAGADDYVV
                     KPFSVKELLARVEAVLRRTPSRPADLAEVGIPGGTVDFARRELRFQDGGREELSEREV
                     ELLRYLANCSDRAVSRDELLANVWRISPDGLPTRTIDMHVARLREKLRDDPAQPHVLL
                     TVRGKGYMFQTTNDK"
     gene            3911..5917
                     /locus_tag="VFV87_18935"
     CDS             3911..5917
                     /locus_tag="VFV87_18935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5105905.1"
                     /translation="MKRPWQIWLLFLLGLAAVVPAFAWLTVKALELDRAESLARRQAA
                     LEKDISVALWRMDVLLTPLLAEEAARPDFVYRAVYPTDDAPEPAKGKPAEPALSLSPL
                     LANPPSFVLLHFEVHPDGSVSSPQNPTGRENTWAVDNGISPQFIGQAANRLQELEPVL
                     APAVLLAQLPDEPQTLAEPPGTTFAASVPPPAGQNTVISNTYEDLNQQLAQSEGPPPQ
                     PNPPPQSDSGQQAVRPVAPPNDGEQAPNQASDFSAQPQGGNQLAYSQNTEPSRQQQRL
                     QSQIANNDLANRGAALQAYALRSAAEQRGNYKAPPKTVRVVEGVSRRFWLGERLIQAR
                     RVQIGDETIIQGCWLDWNALQTRLKAEVADLLPAVELRPVSIDDPEALEGHLLATLPV
                     QLAVTSPLLLDAGWTPIRISLVVAWACLLVTVLAAAMMLHSVITLSERRGAFVSAVTH
                     ELRTPLTTFRMYAEMLAGGMVPEADQRQRYLETLRVEADRLAHLVENVLQYARLERGR
                     PGKRREEVSIASLLDRCESRLAERAAQAEMRLEVEAAEADREVTLATDSAAVEQIVFN
                     LVDNACKYAGQANDKRIHLEIAADARRVRIAVRDHGPGISRAGRKKLFRPFSKSVHEA
                     ASTAPGVGLGLALCRRLAADLGGRLDLESPAESGAAFVLTLPLS"
     gene            complement(6448..>6977)
                     /locus_tag="VFV87_18940"
     CDS             complement(6448..>6977)
                     /locus_tag="VFV87_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5105906.1"
                     /translation="LVASPHLSDPNFYKSVVLMIKHDDEGAFGLILNRPTGSTVREIW
                     QAVGEQDLECMDPIYLGGPVSGPLVCLHRIKSCGEAEVLKGVYFSAHKEQLLKIASQS
                     SKPFRLFSGYAGWAGGQLEGELKVGGWLSLPATKDLIFQDSSTLWDEVVRTIGDEILG
                     PAIKTKHVPQNPSLN"
BASE COUNT         1286 a         2231 c         2239 g         1221 t
ORIGIN      
        1 ggcggcaagc atcgagttgt agccgtccaa tcgggctttc agcgcgagga cgcgccccat
       61 cgcgaggtcg aactccgctt gccagcgggg actagtgagc ttctcgcggt cgccttcgcc
      121 cggcgccagc acgttgtaga gccgatcgac ggcaggcgcg tacttggccg cgaattgctg
      181 ggcgctgttc agattgcggg cattctgggc ttcggtcccc ttggggaagg agaggctcgg
      241 atagtcttcg acaacgacct tcggcagcct ggccgcttcg tggagggcca ggcgggcctt
      301 gttttccgcg agcaactggc ggtactccgc ttcgctgacg taatcggggg cgtaacggct
      361 ctggctttca gtggtaaacc gcagctcatt tcccggcggc caatattgat acgtctcgcc
      421 gcggtacgaa taggcggaac tccccgcctc gggacgaatc gcaaagtatt gcccgcggct
      481 ggcgcggcac aggcgctcca gggcgaaggg cccgaagcct gagtcgatca aggcgaaatc
      541 gggccgctgg ccggaatcaa agccgtagct ggccgcccag ccctggatat cgacgcgttc
      601 cgagaagagc gattccggac cgtgcaccgg agcggaatcg tccatcttgg ttgtatcgat
      661 cgccttggga tcggcagcga acggattgac ttgcccccac ggcgctggcg agccaatgac
      721 atagatcgga atggcctgcc gccgcgtgct ttcggccagt tcttcggcaa gctgcggatc
      781 gtcgcccgct tcgtcggtga tgacgaggag gagcaattcg cggcgctgat cggtgcggaa
      841 cgtcaggtat ttttccagcg cctgcttgat tgctgcgaac gtgttttcgc ggccggcggt
      901 ggacggctga atgccgtccc acgcggcttt gactttttgc cagtcgcccg tcggcggatc
      961 gaggagcagt tctggcgtct cgtcataggc gatgactgcc gtcaacagcg gatcatcctc
     1021 ggacagcgac cactgccgca cgtcctgcga atcgtagtaa ttcttcaccg cggaagtggc
     1081 ctgcgcgacg atcttctggc tgctgggggt gcgatcgacg atccagacga cgagcgtcgg
     1141 cccgatttcg cggctggcct tgatcgccag gccgaccttc ttcacggcta cgtcttcagc
     1201 ccggccgata ttgatcgagc cgctccgcgt cgcctgataa gcccgcgcga tcacggccaa
     1261 ctcatcctgc gcgtcttgct gcttgggctg atccggcagc ttgggccggg cgatcttgcg
     1321 gttcttgaag tcggcctgcc gctgggcctc gtcgcgcagc ctgttgccgc cgacgtcgtg
     1381 gcagccgggc agcagagcta gggccagaat cgctgcgatg gtgagcgaaa cgcgatgtga
     1441 cgatgaagtc atcggatcat tttaacttgg ctacttaaac agagcacgcg ggattggctg
     1501 gcagacaaga atgtctgacc cacgcgcctg gttacaaacg ctttacacgc ggcgggaggc
     1561 ttcgcgagta ggattgccga ggatgaaggt gggcccagtt tcacgtattg aagaggagca
     1621 tcggccatgc ggcgaattct aatgacggca atgatcgggt gcctggcact ggcctgggcg
     1681 gcaccagcgc gggcctgcgg cagctacctg cggatgcaag tcctccaggc gctcgcagac
     1741 ggcgcgaatg ccgctacgat cgaggagttg cgggcagccg ggccggcagc gctcgatgaa
     1801 ctatttcagc tccgcgacca gatgacgaaa tcgctgggcg aaaaaaccag tcagccgacg
     1861 ccaacagcag acgaagttat tcagatcgag agactgcagc agcggatcca gcgcctggac
     1921 gagatgatcg accaggtcgg catgcagcgc tattgctcgc ggtcgcgact ctactggtac
     1981 accgacttcg acaaagccgt aaccgccgcc cgtgaaagcg gcaagccgat cctcagcctg
     2041 cggatgctgg gcaacctgaa cgaggacttc tcgtgcgcca acagccgctt cttccggacg
     2101 acgctgtatg ccaatgcgga gatttccaaa acgctgcgcg agaactttgt cttgcactgg
     2161 aagagcgtgc gtcccgttcc gagagtgacc atcgacttcg gcgacggccg caagctggaa
     2221 cgcaccgtca ctggcaatag cgcccactac atcctcacgc ccgatggcga agtggtcgat
     2281 tgcttgcctg ggctgtatgg gccgaaggcg ttcctgcgaa agatcaacga ggggctctcg
     2341 atggccaggc aagtcgcggc cttgtcgccg agcgagcgcg gcgagcgact ggcggctttc
     2401 catgccaaag agctcgccga cctgcaatcg gccttcgggc gagatctaat tctcgtgtca
     2461 gccgccaatg accgggcgct gacagcggcg ccgcagccgt cgcagcagac gcgggctgcc
     2521 ccgccggcaa atgtccccac ctccctcgcg cggcccaaag cacgcatcga aacgcggctt
     2581 gtcgctgccg ccatcccgac cgccgccgat tcgctgtcgc tcgacgatga cgccgtctgg
     2641 cagcggatcg ccgcgctgca tgccgaagac gcccagcttg atagtgccag ccgcgagctg
     2701 atcaagagcg aaaaccccac cgccgcggtc gccggccgcc tggccatgac caaggccctg
     2761 gtcgagaacc cgctggtgcg gctggtgcgg tcgctgcagg aatcgatcgg cctcgacacg
     2821 gtgaagaacg agtaccgctt ccaccggcag attcacggct ggctggcgga cgggaattac
     2881 cggcccggca tcgacgagct gaacgagcgg gtctatgccg aactttttct cacccccagc
     2941 agcgatccgt ggctcggcct ggcgccgccc gatgcctata cggccctgcc caatggcggc
     3001 gtcgtggcca atcgcggccc gtagcgcaag cgagggaatt gtgggtgcat aagcaattcg
     3061 ggcaggactc tttggtcgtt agaatgattg gaggtgtgcc gagcaccagg cgaacgggaa
     3121 atgggaagtc cattaccctc gcttgcgcta cgggctaaga ttcgatgccc aagaccattc
     3181 tcactgtcga agacgatgcg gccatccgcc gggggatcgt cgatgcgctg aagtacgttg
     3241 gctacggcgt actggaggcg ggaaatggga acctcgggca cgaaatggcg gtgcagcagg
     3301 cgtatgattt gctgctgctc gatttggtgc tgcccggccg cggcggcttg gagatcctgc
     3361 gggccgtgcg gaccacgcga ccgacgcagc cggtgatcat tctgaccgct cgcggcgaag
     3421 agaacgaccg ggtcgagggc ctgaaagccg gggcggacga ttacgtcgtc aagccgttca
     3481 gcgtgaagga attgctggcg cgggttgagg cggttctgcg gcggacgccc agccggccgg
     3541 ccgatctggc cgaggtcggc attcccggcg gaacggtcga tttcgcccgc cgcgagttgc
     3601 gcttccagga cggcgggcgg gaagaactgt cggaacgcga ggttgagctg ctgcggtatc
     3661 tcgccaactg ctccgaccgc gccgtcagcc gcgatgagct gctcgcgaac gtctggcgga
     3721 tcagccccga cggcctgccg acgcggacga tcgacatgca cgtcgcccgc ctccgcgaaa
     3781 agctccgcga cgacccggcc cagccgcacg tgctgctgac ggtgcggggg aagggatata
     3841 tgtttcagac tacaaatgac aagtgacgag tgacaaatga caacgcggtg attggtcact
     3901 ggtatttgca atgaagcgac cgtggcaaat ctggctcctc tttctcctcg gcctggcggc
     3961 tgtggtgccg gcgtttgcct ggctgacagt caaggcgctg gagctcgacc gggcggaatc
     4021 gctggcccgg cggcaggcgg cgctggagaa ggacatcagc gtcgcgctgt ggcgaatgga
     4081 tgtgctcctg acaccgcttt tggcggagga ggcagcccgg cccgacttcg tttatcgggc
     4141 cgtctatccc accgacgatg ctccggagcc ggccaaaggg aagcccgcgg agccggcgtt
     4201 gtcgctttcg ccgctgttgg cgaatccgcc gagcttcgtc ctgttgcact ttgaggtcca
     4261 tccggacggc agcgtcagct cgccgcagaa cccgacgggc cgagagaaca cctgggcggt
     4321 cgataacggc atctcgccgc agttcatcgg ccaggccgcg aatcgactgc aagagcttga
     4381 gccggtactc gcgcccgcag tgcttctggc ccagcttccc gatgagccgc agactttggc
     4441 cgagccgccg gggactacgt tcgccgccag cgtccctccg cccgccggac aaaacacagt
     4501 catctcgaac acctacgagg acctcaatca gcagctcgcc cagagcgaag gtccgccgcc
     4561 acagccgaac ccgccgccgc agagcgacag cggccagcag gcggtgcggc cagtcgctcc
     4621 gccgaatgac ggcgagcaag cgccgaatca agccagcgat ttttcggcgc aaccgcaggg
     4681 cgggaaccag ctcgcgtaca gtcagaacac cgaaccttcc cgccagcagc agcggctgca
     4741 gtcgcagatc gccaacaacg acctggccaa ccgcggggcg gctttgcagg cctatgccct
     4801 ccgctccgca gccgagcagc ggggcaacta caaggccccg cccaagaccg tccgcgtcgt
     4861 cgaaggcgtc agccgcaggt tctggctggg cgagcggctg attcaagccc gtcgcgtgca
     4921 aatcggcgat gagacgatca tccaagggtg ctggctcgat tggaacgcac tacaaactcg
     4981 tttgaaggca gaagtcgccg acctcttgcc ggccgtcgag ttgcgaccgg tctcgatcga
     5041 cgatccggaa gccttggagg ggcatttgct cgcgacgctg ccggtgcagt tggctgttac
     5101 atcgccgctg ttgctcgacg ccggctggac gccaatccgt atttcgctcg ttgttgcctg
     5161 ggcgtgcctg ctggtgaccg tgctggccgc ggcgatgatg ctccacagtg tcatcacgct
     5221 cagcgagcgc cgcggggcct tcgtctcggc ggtgacgcac gaattgcgga cgccgctaac
     5281 gacgttccgc atgtatgccg aaatgctcgc tggcggcatg gttcccgaag ccgaccagcg
     5341 gcaacgctac ctggaaacgc tccgcgtcga agccgaccgg ctcgcgcacc tggtcgagaa
     5401 cgtgctgcaa tacgcccggc tggagcgtgg ccggcccggg aagcggcggg aagaggtatc
     5461 catcgcctct ttgctcgacc gctgcgaatc gcgcctcgcc gaacgcgccg cacaagccga
     5521 aatgaggctc gaagtcgaag ccgccgaagc cgaccgcgag gtcacgctgg ctacggattc
     5581 cgccgcggtc gagcagatcg tgttcaatct ggtcgataac gcctgcaaat acgcgggcca
     5641 ggcgaacgac aagcggatcc acctcgaaat tgccgccgac gcgcgccgcg tgcgaatcgc
     5701 cgtccgcgat cacgggccgg gcatctcccg cgccggtcgc aagaagctct tccgcccctt
     5761 ctccaaatcg gtccacgaag cggccagcac ggcccccggc gtcggcctgg gcctggccct
     5821 ctgccgccgc ctcgccgccg atctcggcgg ccgactcgat ctcgaatcgc ccgccgaaag
     5881 tggcgcggcc tttgtgctca cgcttccgct ctcttagccg ggagcaaacc tgctcccggc
     5941 gtgcagcatg ttcagcatgg tcgcacgtgg cctggccaac tttgaggagc aacctcaatt
     6001 tcgacatcgt cgagccagat gggttcagct tcgcgcgatc cagataatcc cgattaggat
     6061 gaagaccgca ccacgagcta agtcgtagaa gctaaggcca tcggtaccga caagcgtcag
     6121 cacgatgtat ccaaccccgg aaacgacgag cattggtccc acccatcgaa tcgcacgctg
     6181 ccgccgcaga tgatcgcgcg cagcgccttc cgcgcgattg atcgaccgcc ccaccggcag
     6241 catttcactt ggcgcaccac aggaggggca atggttcggc ctatactcaa ttggtttggc
     6301 agtacattgc aaatgaccag ggcagcggca ctcgggacag aattgcgctt ccattgcatt
     6361 gctaattcgc gatccgcaga tcacgcaatg ccgtccaacg agttgcatgt gctcagctcc
     6421 aacgcgttgg tcatccgcga ccgagtccta attcagcgaa gggttctgcg gcacgtgctt
     6481 cgtcttgatg gccggaccga gaatctcgtc gccgatcgtg cggacgactt cgtcccagag
     6541 cgtgctgctg tcctgaaaga tcaggtcctt ggtggccggc agcgagagcc agccgccgac
     6601 cttcaactcg ccttcgagct ggccgccggc ccagccggca tagccagaga acaggcggaa
     6661 tggcttgctc gactgcgagg cgatcttcag aagctgctcc ttatgggccg agaaataaac
     6721 gcccttgagc acctccgcct cgccgcacga tttgatccgg tgcaggcaga ccagcggccc
     6781 gctcaccgga ccgccgaggt aaatcgggtc catgcactcc aaatcctgct cgccgaccgc
     6841 ctgccagatt tcgcggacgg tgctgccggt cgggcggttg agaatgaggc cgaacgcgcc
     6901 ttcgtcgtcg tgcttgatca tcagcacgac cgacttgtag aaattcggat cgctcaagtg
     6961 cggcgatgcg acgagaa
//
LOCUS       DASUGB010001001         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1916786, whole genome shotgun sequence.
ACCESSION   DASUGB010001001 DASUGB010000000
VERSION     DASUGB010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916786"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFV87_18945"
     CDS             complement(<1..1284)
                     /locus_tag="VFV87_18945"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX5105907.1"
                     /translation="MSHATPTRQQGSAVWCLAIAMSAAGAWQARAELPSIRFDRLQPL
                     GLAAGGSVEAEVNGADLEGLASLRFDQPGLSAEPIDGKERWFRITAAADVPAGTYDVR
                     LVGRFGVSSSKLLQVQRGLAEVPEKEPNNELAQSQAVPVNCVIGGASDGENIDLYRFT
                     AKAGQRIVLDVDAQKLDSMLDSQLLVLNSTGKQLASSGDYHGRDSLIDFVAPADGEYI
                     AVLHDLSYRGGYPYRLTISDRPHVENVFPRAIQRGQTAQLTALGRNLGGGPAAVGATG
                     EPVLESASFAVTADVPPGQFRYLEHPTDHSVLPTAATFRLDGLQVRPALPAGESLTPV
                     TLLVVDEPVALEVEPNDTKDAPQVINLPAVVSGRFDQPRDADWFAFTSPEDGQYAIEV
                     YAERLAAQADPYVVVLDEQGGRVSELDDYGHRINAF"
     gene            complement(1281..>2287)
                     /locus_tag="VFV87_18950"
     CDS             complement(1281..>2287)
                     /locus_tag="VFV87_18950"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105908.1"
                     /translation="LALVRSAFHTNAGHGMGSQWMLTGWQPTIEVSDNIFPSCGSVVS
                     KMLGANEPGLPAYVNLPRQLGLGKAAYLGASHNPFSPEDDPNNDNFQVKNLKLPGRVG
                     ADRLARRQQVLGQIDQIRRDIDTKGDLAGIDTFYRDALEMVTSDKAQKAFHIQSEDAK
                     LREKYGRNDLGQSCLLARRLVEAGVTYVAIQAGGGWDTHGDNFSALKNRLLPNYDRAL
                     AALVSDLYDRGLSHDVLVMAMGEFGRTPRINGGAGRDHWPGAMTIVYAGGGLTMGQAI
                     GTTNDKAEFPTTKAYTVGCVLSTMYHTLGIDHRHAFYDQAQRPLPILNEGQPIEELVG
                     "
BASE COUNT          375 a          738 c          770 g          404 t
ORIGIN      
        1 gaaggcattg atccggtggc cgtagtcgtc cagctcgctg acgcggcccc cctgctcatc
       61 cagcacgacc acgtagggat cggcctgagc cgccagccgc tcggcataaa cttcaatggc
      121 gtattggcca tcttccggtg acgtgaaggc gaaccagtcg gcgtcgcgcg gctgatcgaa
      181 ccggccactg acgacggcgg gcaggttgat cacctggggc gcgtccttcg tgtcgttggg
      241 ctcgacttcg agcgcgaccg gctcatcgac gaccagcagc gtcaccggcg tgagcgattc
      301 gccagcaggc agtgcagggc gcacctgcag gccgtccagg cggaacgtcg cggccgtcgg
      361 caggacgctg tgatcggtgg gatgttccag gtagcggaat tggcccggcg gcacgtcggc
      421 tgtgacggcg aagctcgccg attcgagcac cggctcgccg gtggcgccga ccgccgccgg
      481 cccgccgccc agattccggc cgagagcagt caattgcgcc gtctgcccgc gctgaatcgc
      541 tcggggaaag acgttctcga cgtgcggccg gtcgctgatc gtcagccgat acggatagcc
      601 gccgcgatag gacaggtcat gaaggacggc gatgtactcg ccgtcggccg gagcgacaaa
      661 gtcgatcagc gagtcgcggc cgtgatagtc gccgctggaa gcgagctgtt tgcccgtgct
      721 attgagcacc agcaactgcg aatcgagcat cgaatccagc ttctgagcat cgacatccag
      781 cacgatccgc tggcccgcct tcgcagtaaa acgatagaga tcgatattct cgccatcgga
      841 ggcgccgcca atcacgcaat tcaccgggac tgcctgcgac tgtgcgagct cgttgttggg
      901 ctccttttcg ggcacttcgg ccaggccgcg ctggacctgc aggagcttcg agctgctgac
      961 gccaaatcgc ccgacgaggc gcacgtcgta ggttcccgcc ggcacgtcgg ccgcggccgt
     1021 gatcctgaac caccgctcct tgccgtcgat cggctcggcg gacaggcccg gctgatcgaa
     1081 ccgcagggag gcgaggcctt ccagatcggc cccgttgacc tcagcttcca cgctgccgcc
     1141 ggcggccagg ccgagcggct ggaggcggtc gaagcggata gagggaagct cggcgcgggc
     1201 ctgccacgct ccggcggccg acatcgctat tgccagacac cacacggcac tcccttgctg
     1261 gcgcgtcgga gtggcgtgac tcatccgact aattcctcga tcggctggcc ttcgttcagg
     1321 atcggcagcg ggcgctgggc ctggtcgtag aaggcgtgcc ggtggtcaat gccaagcgtg
     1381 tggtacatgg tcgagaggac gcagccgacc gtgtaggcct tcgtcgtggg gaattccgcc
     1441 ttgtcgttgg tcgtgccgat cgcctggccc atggtcaggc cgccgccggc atagacgatc
     1501 gtcatggcgc cgggccaatg gtcgcggcct gcgccgccgt tgatccgcgg cgtgcggccg
     1561 aattcgccca tcgccatcac cagcacgtca tgcgagaggc cgcggtcgta aagatcggaa
     1621 acgagggccg cgagcgcgcg gtcataattc ggcagcaggc ggttcttgag cgcggagaaa
     1681 ttgtcgccgt gcgtatccca gccgccgccg gcttggatgg cgacgtaagt aacgcccgcc
     1741 tccaccagcc gccgcgcgag cagacagctc tgccccagat cgttccggcc gtatttttcg
     1801 cggagcttgg catcctcgct ttgaatgtga aacgcctttt gggccttgtc gctggtgacc
     1861 atttccagcg cgtcgcgata gaacgtatcg atgccagcca ggtccccctt ggtgtcgatg
     1921 tcgcggcgga tctggtcgat ctggccgagc acttgctggc gtcgggccag ccgatcggcg
     1981 ccgacccggc cggggagctt caggttcttc acctggaagt tgtcgttgtt cggatcatct
     2041 tcgggcgaga acggattgtg cgacgcaccc agataggccg ccttgcccag gccaagctgt
     2101 cgaggcaagt tcacataggc aggcaggccg ggctcgttgg cgccaagcat tttgctgacg
     2161 acgctgccgc acgacgggaa gatgttgtcg ctgacctcga tcgtcggctg ccagccggtg
     2221 agcatccact gcgagcccat gccgtgaccg gcgttcgtgt ggaacgccga gcggacgagg
     2281 gccagtt
//
LOCUS       DASUGB010001002         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1361594, whole genome shotgun sequence.
ACCESSION   DASUGB010001002 DASUGB010000000
VERSION     DASUGB010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361594"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VFV87_18955"
     CDS             <1..1404
                     /locus_tag="VFV87_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5105909.1"
                     /translation="WQASTDGMYDKVIMRDDLTWSDGKPITAHDIAYSFQVILTRAVP
                     IPAVRQGTSQLKWVEAYDDQTVVFFHKEPLATNTMNINFPIIPKHVYEESLPSDPTMT
                     RNPKHVELEDRPVVGGAYEIKSRSRGTDIVLERRESYYMHDGKQVRDKPYFKTVRFRI
                     QTDPSTAILALKAGDIDEMEITPEQFKTQTNDDEFYKLNTKVYATEWTSFHFLWNCKS
                     PLFADKRVRTAMSYAFDHEELLSTLLFGMCEPCTGNFHPDSKWAPENPPQPYEQDLDK
                     AEELLDEAGWTDSNGDGIRDKTINGKRVDFRFSVITSNRPDRIAICQLLERSLDQIGI
                     ECNVSAMEFPVVIEKLQKHDFQAAFGGWGSGTDPDTSENIWATGQNRNYGEYNNPEID
                     ELFVQGRKELDPAKRPEIYRQIHQKLWEDQPYTWLYYRNAFYGFNKSVRGYYFSPRGP
                     YNFGPGFSSIYKVESKQ"
     gene            1457..2443
                     /locus_tag="VFV87_18960"
     CDS             1457..2443
                     /locus_tag="VFV87_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105910.1"
                     /translation="MTSYLIRRLMFAAFTLLVFTALMYALIRNIPGTPLTLVGAEDPS
                     HKISAEDMRLLNAAYGLDKPWYRAYGDWLANIARLDLGTSFRFRKSVAGLILERLGPT
                     LLLSVPAFIITYLLAIPIGLYMTRKSGQASERILSVLFYMLYSLPTYVAALWLLYWFY
                     LRLHGTIFQLSPGMTSDNYDELSAGGKLLDLGRHLLLPLLCYSYGALAYDTRFIKANM
                     EEAIRQDYVRTARAKGVAPGRVLWLHAFRNTLIPFVTIIGLTLPGLISGAVILEQIFS
                     WPGIGKLFYESITFNDYPLIMGLTFMFAVATLLSQLLADILYAFVDPRITYT"
     gene            2447..3565
                     /locus_tag="VFV87_18965"
     CDS             2447..3565
                     /locus_tag="VFV87_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5105911.1"
                     /translation="MSADKRSHSSAPPALEHPRPAKSRGYWAETWRRFRKKPLPMIAL
                     AYVLLLGVVGLLSPAIAGTKPVMCYYKGRIYFPCLGYFRERWENPIFTTDKFRNQYYK
                     NLPKKDPDSWAVWPLVFQDPYERLRKDWREGYPENPIGDLGKPNAYNLMGTTTDGKDV
                     FSHLIHGTRLALLVGFVSVGIAAAIGITLGALAGYFGGLLDMLLSRLIEIVMCIPTLI
                     LILALIAVLERPTVWHTMAILGVTGWPGIARLTRAEFLKLKALDYVSAARAMGAGHIR
                     IIFRHILPNALAPVLVPITFGIAAAILIENALRFLGLGDLSGASWGLLLNEGRENLKM
                     WWLIVFPGTAIFLTVMAYNLIGEGLQEATDPRLRESAK"
     gene            3580..>4812
                     /locus_tag="VFV87_18970"
     CDS             3580..>4812
                     /locus_tag="VFV87_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5105912.1"
                     /translation="MSTTLLEIDNLRTYFHTDDGVVRAVDGISLQIKEGQTLGVVGES
                     GSGKSVTAFSVMRLLAGTAKIETGRIALLGQNLVKLPEADMRKIRGKEISMIFQEPMT
                     SLNPVFTVGWQVMEAIMLHQKVSKAEARKRSIELFREVGIPDPETRIDSYPHQMSGGQ
                     KQRVMIAMALSCNPKLLIADEPSTALDVTIQAQILDILRQLRDQRGMSVLFITHDLGV
                     IAEIADEVAVMFQGKIVEYGKVLEIFERPQHPYTKGLLACKPKLQTNYKVLPTVADFM
                     EVVEEKDGQTRIVEKKITPERLEQLTTQGRGRLLHPQSKLAEMGHPWEEEKHPPDTKT
                     VPEDQPPLLSVKGLKVYFPIRRGIFLRTVGHVKAVDGVSFDVYRGQTLGLVGESGCGK
                     TTAGRAILRLIEPTAGRVI"
BASE COUNT         1000 a         1489 c         1384 g          939 t
ORIGIN      
        1 tggcaagcca gcacggatgg gatgtacgac aaagtcatca tgcgcgacga cctgacgtgg
       61 tcggacggca agccgattac ggcgcacgac atcgcctaca gcttccaagt gatcctgacg
      121 cgcgcggtgc cgattcccgc ggtgcgccag gggacgagcc agctcaagtg ggtcgaggcg
      181 tatgacgacc aaacggttgt gttcttccac aaggagccac tggcaacgaa cacaatgaac
      241 atcaatttcc cgatcattcc caagcacgtt tacgaagagt ccctgcctag tgatcccacg
      301 atgacgcgaa accccaagca tgtcgagctg gaagacaggc cagtcgtggg cggcgcctat
      361 gagatcaaat ctcgcagccg cgggacggac atcgtcttgg aacgccgcga aagttattac
      421 atgcacgacg ggaagcaggt ccgcgacaag ccgtacttca agacggtccg cttccgaatt
      481 caaaccgacc cttccacggc tatcctggcg ctgaaggcgg gtgacatcga cgaaatggaa
      541 attacgccgg agcaattcaa aacgcagacc aacgacgacg agttctacaa gctgaacacc
      601 aaggtctatg ccactgagtg gacaagcttt cacttccttt ggaattgcaa gagtccactc
      661 tttgccgaca agcgcgtgcg aaccgcgatg tcgtacgcgt tcgaccatga ggagctactc
      721 agcacgctct tgttcggcat gtgtgagccc tgcaccggga attttcatcc cgatagcaag
      781 tgggcgcccg agaatccgcc gcagccctac gagcaagatt tagacaaggc tgaggaactg
      841 ctcgatgaag caggctggac ggacagcaac ggtgacggaa ttcgcgacaa gactatcaac
      901 ggcaagaggg tcgatttccg gttttccgtc attaccagca accgccccga ccgtatcgcg
      961 atctgccaac tcctggagcg ctcgctcgac cagatcggca ttgaatgcaa cgtcagtgcc
     1021 atggagtttc ccgtcgtgat cgaaaagctt cagaagcacg acttccaggc cgcgtttggc
     1081 ggctggggca gcgggaccga tcccgacacc agcgaaaaca tctgggcgac aggacagaac
     1141 cgcaactacg gggaatacaa caatcctgaa attgacgagt tgtttgtcca agggcgcaag
     1201 gagctcgacc cggccaagcg cccagaaatc tatcgccaga ttcaccagaa actctgggag
     1261 gaccagcctt atacgtggct ctactatcgc aatgcctttt acggattcaa caaatccgta
     1321 cgcggctact acttcagccc gcgggggccc tacaacttcg gccccggctt tagcagcatt
     1381 tacaaagtcg aatcgaaaca atagttcgct catggtccgc cctggggctg cctcgcggca
     1441 gcaaagctga tcgctgatga cttcctactt gattcgacgc ctgatgttcg cggcgttcac
     1501 cctgctcgtg ttcaccgcgc tcatgtacgc gctgatccga aatatccccg gcacgcccct
     1561 gacgctcgtc ggcgccgaag atcccagtca caaaatcagt gcggaggata tgcggctgct
     1621 caatgccgcc tatgggctcg ataaaccctg gtaccgggcc tacggggatt ggctcgccaa
     1681 cattgcgcgg ctggacttgg gcacgtcttt tcggtttcgc aagtcggtgg ccgggctgat
     1741 cctggagcgt ctcggcccca cgctcctgct ctcggtgccg gcctttatca tcacctactt
     1801 gttggccatc cccatcggcc tttatatgac gcgcaagagc ggccaggctt ccgagcgaat
     1861 tctgagtgtg ctgttctaca tgctctactc gctgccgacc tatgtggccg ccctgtggct
     1921 gctttattgg ttctatcttc gcttgcacgg gaccatcttt caactgtcgc cgggcatgac
     1981 cagcgacaac tacgacgaat tgtcggctgg cggaaaactg ctcgacttgg gccgccacct
     2041 actcctgccg ctcttgtgct acagctatgg cgccctggcg tacgacaccc ggttcatcaa
     2101 ggccaatatg gaagaggcca tccgtcagga ctacgttcgc accgcccgcg ccaagggcgt
     2161 ggccccagga cgcgtgctct ggctacacgc cttccgcaat acgctaattc ccttcgtcac
     2221 gatcatcgga ctgacgcttc cggggctcat cagcggcgcg gtgattctag aacaaatctt
     2281 ctcgtggccc ggcatcggca agttgttcta tgaatcgatt acgttcaacg attatccgct
     2341 gatcatgggg ctgaccttca tgtttgccgt ggccaccctt ctgtcgcaac tgctggccga
     2401 catcctctac gcgtttgtcg atcctcgcat tacctacacc tagtccatgt cagccgacaa
     2461 gcgcagccat tcctccgctc cacccgcgct cgaacatcct cgcccggcga agtcgcgcgg
     2521 gtactgggcg gaaacctggc ggcgctttcg caaaaagcca ctgccgatga tcgccttggc
     2581 gtatgtcctg ctgcttggcg tggtcggcct gctctcaccc gctattgccg gaaccaagcc
     2641 ggttatgtgc tattacaagg gccgcatcta ttttccgtgt ctaggctatt tccgggagcg
     2701 ctgggaaaac cccatcttca ccaccgacaa attccgcaac cagtattaca agaacttgcc
     2761 gaagaaagat ccggacagtt gggcggtctg gccgctggtg tttcaagatc cctatgagcg
     2821 attgcgtaaa gactggcgag agggctatcc cgagaatccc atcggggacc tgggaaagcc
     2881 gaatgcctac aacctgatgg gcaccaccac cgacggcaag gacgtcttct ctcacctgat
     2941 tcatggcacg cgcctggcgc tgctcgtcgg ctttgtatcg gtgggaatcg ccgcggccat
     3001 cggcattacg ctcggcgcgc tcgccggcta tttcggcgga ctgctggaca tgctcctcag
     3061 ccggctgatc gagatcgtga tgtgcattcc cacgctgatc ttgatcctgg ccttgattgc
     3121 cgtgttggaa cgtcccacgg tctggcacac gatggccatt ctgggtgtca ccggctggcc
     3181 gggaattgcc cgcctgacgc gtgcggaatt cctcaagctc aaggccctgg attatgtatc
     3241 cgcggcccgc gcgatgggcg ccggccacat tcgcattatt ttccgccaca tccttcccaa
     3301 cgcgctcgcg ccagtgctcg tgcccattac gttcggcatc gcggcggcaa ttctgatcga
     3361 aaatgccctg cggttcctcg gcctgggcga tttgagcggc gcaagctggg gcctcttgct
     3421 caacgagggc cgcgagaacc tgaaaatgtg gtggctgatt gtctttcccg gcacggcgat
     3481 cttcctcacc gtcatggcct ataacctcat cggcgagggg ctgcaggaag cgaccgaccc
     3541 gcgcctccgc gaaagtgcga agtagctacg acgaaaacaa tgtccaccac gctcctcgaa
     3601 atcgacaacc tccgcacgta tttccacacc gacgacggcg tcgtgcgggc ggtggatggg
     3661 atttcgctcc agatcaaaga aggccaaacg cttggcgtcg tcggcgagtc ggggtcgggg
     3721 aagagcgtga cggcgttttc ggtgatgcgg ctgttggcgg ggacggcgaa gatcgagacg
     3781 gggcggattg cacttctggg gcagaacctg gtcaagctgc cggaagcgga catgcggaaa
     3841 atccgcggca aagagatcag catgatcttt caggagccga tgacctcgct caacccggtg
     3901 ttcaccgtcg gctggcaggt gatggaggcg atcatgctcc atcaaaaggt cagcaaagcc
     3961 gaggcccgca agcggtcgat cgagttgttt cgcgaggtcg gcattccgga ccccgaaacg
     4021 cggatcgatt cgtatccgca ccagatgtcc ggcggccaga agcagcgggt gatgatcgcg
     4081 atggcccttt cgtgcaatcc caagctgctg atcgccgacg agccgagcac cgccctggat
     4141 gtgacgatcc aggcccagat tctcgacatc ctccgccagc ttcgcgatca gcggggcatg
     4201 agcgtgctgt tcatcacgca cgacctgggc gtgatcgccg aaatcgcgga cgaagtggcc
     4261 gtcatgttcc agggcaaaat cgtcgagtac ggcaaagtgc tcgagatctt cgagcggccg
     4321 cagcacccct ataccaaggg cctcctcgcc tgcaagccga agctgcagac caactacaaa
     4381 gtgctgccga cggtggccga cttcatggag gtcgtcgaag aaaaggacgg ccagacacgg
     4441 atcgtcgaga agaaaatcac ccccgagcga ctggagcagc tcacgacgca gggccgcggc
     4501 cgcctgctcc atccccagag caagctggcc gaaatgggcc atccgtggga agaagagaag
     4561 cacccacctg acacgaagac cgtccccgag gatcagccgc cgcttctgtc cgtcaagggc
     4621 ctgaaagtct atttcccgat ccgccgcggc atcttcctgc ggacggtggg ccacgtgaag
     4681 gcggtcgatg gcgtcagctt cgacgtctat cgcggccaga cgctcggcct ggtgggcgag
     4741 agcggctgcg gcaagacgac cgccggccgg gcgattctgc ggctcatcga gcccacggcc
     4801 ggccgcgtga tc
//
LOCUS       DASUGB010001003         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1222711, whole genome shotgun sequence.
ACCESSION   DASUGB010001003 DASUGB010000000
VERSION     DASUGB010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222711"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1744
                     /gene="tatC"
                     /locus_tag="VFV87_18975"
     CDS             260..1744
                     /gene="tatC"
                     /locus_tag="VFV87_18975"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5105913.1"
                     /translation="MAKSKTTRKANDDLFEDTTMTFGEHLEELRVCLFRSVIGIALGC
                     IVGFFVANGVVRFFQGPLEDAMERYYIDKAITDFRDKFDVVPVEVRNMILHEGMIPES
                     IQVETGAVFRSLNLSYPGQLAGFEASPYWFTAGDFLPADTLREEGAKSLARALAGDHG
                     DQDTPAGQLWKLLPEADRLTLEKLAKVETKFSEEQKAQVAAILNRAAGLKELHETPAF
                     LAIEGPDAGPKDTIQSWIASLLGFAASAEYNTVGDLRKQLGERFDPEASRRLNKILLS
                     RAFPDALHKTRLNLLTLITWRPVKVRFQVLNAQEAFMIWMKAALVTGLVIASPYVFYQ
                     IWNFVAAGLYPHEKNYVYLYLPISILLFFGGAALAFVFVFQPVLDFLFLFNKGMNADF
                     DPRIGEWLSFVLILPIGFGLSFQLPLVMLFINRIGLVSVETYVGQWRVAILTICVVAM
                     VLTPADPISMMLMAVPLCALFVLGIFMCKYMPRGRNPFEEAYEP"
     gene            1885..3525
                     /locus_tag="VFV87_18980"
     CDS             1885..3525
                     /locus_tag="VFV87_18980"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5105914.1"
                     /translation="MSDQPIHEVEEPPLDVRGILLRLGPGLIIAASIVGSGELIATTK
                     TGAEAGFWLLWLIIVGCVIKVFVQVELGRDAIVRGKTTLDALGEVPGPRVNGRGNWLV
                     WYYFLMFAASSAQLGAIIGGVGQAMAISAPVTEDGRQFNRQLDVVARQAVVEAELRTA
                     QKQPAGSEKRSEQVLALQTELGDLAARRAAFENERKTDKPFWTDDKIWATIIAVVTSA
                     LLVVGRYGLIQNLATAMVAIFTLMTVVNVLMLQNTQAWAIRWQDLADGLRFRLPPADA
                     PGGNTALLTALKTFGIIGVGASELISYPYWCLEKGYARFTGPRDKSDSWAARARGWMT
                     VMRWDAWCSMVIYTIATVAFYLLGAAILSRSGLVPEDRDLIRTLNVMYEPVFGSWAKI
                     LFLLGSFAVLYSTFFVANAGNARVFSDALRVMGLAGPSPAAHRIRVKWLSGILPLVCL
                     VLYLIIPTTPAQLVLLSGMMQALMLPMLAGAALYFRYTKSDSRVTPGRLWDAFLWISS
                     IGMLISACVLGYGEVKKFLPKQPAASPPAATQPVEKTP"
     gene            3533..>3718
                     /locus_tag="VFV87_18985"
     CDS             3533..>3718
                     /locus_tag="VFV87_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5105915.1"
                     /translation="MPRYVSHLEGAIDGTRLPADELATLHKDRPILVRYDLPAVARHF
                     SKISLQERPQTLWRYREL"
BASE COUNT          645 a         1157 c         1157 g          759 t
ORIGIN      
        1 catggctgtc ccgaacggat tgtgaattcg ctcaccttga agtaggataa tgctcggctg
       61 tggcattcac cacagccgct cacctggtac ggcagcatcc caccgtacgc ccaccgctgt
      121 tgcggcagct tcctaccgca cgtcccgtcg gatgcattcc gggactctcc tccccagggc
      181 cttacgcacg cgagcgcgtg ccgccgctgg cggattcacg gcacacagag tgtgcctgct
      241 acttttggga gaaacggaca tggccaaatc caaaaccacc agaaaggcga acgacgacct
      301 gttcgaagac acgacaatga ccttcggcga gcacctggag gagctccgcg tctgtctctt
      361 tcgctcggta attgggatcg ccctgggctg catcgtgggc ttcttcgtcg ccaacggcgt
      421 ggtgcggttc tttcagggcc cactcgaaga tgcgatggaa cggtattaca tcgataaggc
      481 tatcaccgac ttccgagata aattcgatgt cgtgccggtc gaagtccgca acatgattct
      541 gcacgagggc atgattccgg agtcgatcca ggtcgagacc ggagcggtgt tcagatcgct
      601 caacctgtcg tatcccgggc agcttgccgg cttcgaggct tcgccctatt ggttcacggc
      661 cggcgatttc ctgccagccg atacgctgcg ggaggaaggt gccaaatcgc tggcccgggc
      721 actggccggc gaccacggcg accaggacac gcccgccggc cagctttgga aactgctgcc
      781 ggaggccgat cgcctgacgc tggaaaagct ggccaaagtc gaaaccaaat tcagcgagga
      841 gcaaaaggcc caggtcgcgg ccatcctcaa ccgcgcggcc ggcctgaagg agctgcacga
      901 aacgccggcc ttcctggcca tcgaagggcc cgatgcgggt ccgaaggaca cgatccaaag
      961 ctggattgca tcgctcctcg gctttgccgc cagcgcggaa tacaacacgg tgggggatct
     1021 gcggaagcag ctcggtgaga gattcgatcc cgaagcctcg cggcggctga acaagatcct
     1081 gctgtcgcga gcgttccccg atgcgctgca caaaacgcgc ctgaacctgc tcacgctgat
     1141 cacctggcgg cccgtcaagg ttcgcttcca ggtgctcaac gcccaggaag cgttcatgat
     1201 ctggatgaag gcggcgctgg tcacgggcct ggtcatcgcc agcccgtacg ttttctatca
     1261 gatctggaac ttcgtcgccg cgggcctgta tccgcacgag aagaactacg tctatctcta
     1321 cctgccgatc agcatcctgc tgtttttcgg cggggcggcg ctggcgtttg tcttcgtctt
     1381 ccagcccgtg ctcgatttcc tcttcctctt caataaggga atgaacgccg acttcgatcc
     1441 ccggatcggc gaatggctca gcttcgtcct catcctgccg atcggctttg ggctgagctt
     1501 tcagctgccg ctcgtcatgt tgttcatcaa ccgcatcggc ctggtgtcgg tggagaccta
     1561 cgtcggccag tggcgggtgg cgatcctgac gatctgcgtc gtcgcgatgg tcctgacgcc
     1621 cgcggatccg atcagcatga tgctgatggc ggtgccgctg tgcgccctgt tcgtcctggg
     1681 gatcttcatg tgcaagtaca tgccccgcgg ccgcaaccca ttcgaagaag catacgagcc
     1741 gtaaaaggac gtcgatgctt ttgcggttcg gacgcgatca gtgtccgcgc gttagaatac
     1801 tgatgttgcg ctgcttttct tcggctgcgt actgcggtac ggtgatcgct gctcccgtcg
     1861 atccttgctt ccaattgcta gtccatgtcc gaccagccga tccatgaggt cgaagagccg
     1921 ccgctggatg tgcgagggat cctgctgcgc ctggggccgg ggctgatcat tgccgcgtcg
     1981 atcgtcggtt cgggcgagct gatcgccacg accaagaccg gggcggaggc cggcttctgg
     2041 ctgctgtggc tgatcatcgt cggctgcgtc atcaaggtct tcgtgcaggt ggagctgggg
     2101 cgcgacgcga tcgtccgcgg caagaccacc ctggatgccc tgggggaagt gcctggaccg
     2161 cgcgtcaacg gccgcggcaa ctggctggtc tggtactact tcctgatgtt cgcggccagc
     2221 tctgcccagc ttggcgccat catcggcggc gttggtcagg ccatggcgat cagtgctcca
     2281 gttacagaag acggccgcca gttcaaccgg cagctcgacg tcgttgccag gcaggcggtc
     2341 gtcgaggcgg aactgaggac ggcccagaag cagcctgcag gcagcgaaaa acgttcggag
     2401 caagtcttgg cgctccaaac agagttgggc gatctcgcgg cgcgccgagc cgccttcgaa
     2461 aacgagcgga aaaccgacaa gcccttctgg acggatgaca aaatctgggc cacgatcatc
     2521 gcggtggtta cgtccgcctt gctggtcgtg gggcgttacg gcctgattca gaaccttgcc
     2581 acggcgatgg tggcaatctt tacgttgatg accgtcgtca acgtgctgat gttgcagaac
     2641 acgcaagcct gggcgatccg ttggcaggac ctggccgacg gcctcagatt tcgcctgccg
     2701 cctgccgatg cgcctggtgg caatacggcc ctgcttactg cgctgaagac gttcggcatc
     2761 atcggggtcg gcgcttcgga gctgatttct tatccctatt ggtgcctgga aaaaggctac
     2821 gcccgcttta caggcccgcg cgacaagagc gactcgtggg ccgcccgcgc ccgcggctgg
     2881 atgaccgtga tgcgctggga tgcgtggtgc tcgatggtca tctacaccat tgcgaccgtc
     2941 gcgttctacc tgctcggcgc ggcgatcctc agccgcagcg gcctggttcc ggaagaccgc
     3001 gacctgattc ggacgctgaa cgttatgtac gagccggtgt tcgggagttg ggcaaaaatc
     3061 ctgttcctgt tggggtcatt cgctgtgctt tactcgacat tcttcgtggc caatgccggc
     3121 aacgcccgcg tcttcagcga cgcgctgcgg gtcatgggtc tggcgggacc atcgcccgcg
     3181 gcccaccgga tccgggtgaa gtggctttca ggcatcttgc cgcttgtgtg cctggtgctg
     3241 tatctgataa ttccgacaac gccggcccag cttgtgcttc ttagcggcat gatgcaagcg
     3301 ctgatgctcc caatgctggc tggggccgcc ctctatttcc gctacacgaa gagcgattcg
     3361 cgggtgacgc ccggccggct gtgggacgcc ttcctgtgga tttcgagcat cgggatgctc
     3421 atctcggcct gcgtgctggg gtacggcgag gtgaagaagt tcctccccaa gcagcccgcc
     3481 gcctcacctc ccgccgcgac gcagccagta gagaagacac cctgagtgat tcatgcctcg
     3541 ctacgtctcc catctcgaag gtgcgatcga cggcactcgc ttgcccgccg atgagctggc
     3601 cacgctgcac aaggaccggc cgatcctggt gcggtacgat ttgccggcgg tggcccgcca
     3661 tttcagcaag atctccctcc aggagcggcc tcagacgctg tggcgctatc gcgagctg
//
LOCUS       DASUGB010001004         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_110761, whole genome shotgun sequence.
ACCESSION   DASUGB010001004 DASUGB010000000
VERSION     DASUGB010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110761"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFV87_18990"
     CDS             complement(<1..831)
                     /locus_tag="VFV87_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX5105916.1"
                     /translation="MARLSRWQDFVLPLGLIASVLVIIVPLPSFMLDLFLAANVTLSV
                     IVLLTTIYVRTPLEFNIFPSMLLATTLCRLVLNVASTRLILTHAGQDGLLAAGGVIKS
                     FGEFVAGDQIVVGIIIFVIIVVIQFVVITKGATRISEVAARFALDGMPGRQMAIDADL
                     NAGVIDEKEAQRRREEITQQADFYGAMDGASKFVRGDAIAGIVITLINILGGFVIGVV
                     QEGMTFTEAAEVFTKLTIGDGLVSQVPAFLISLAAGLLVTRSTQKTDLPVQFLAQLFS
                     N"
     gene            complement(946..1146)
                     /locus_tag="VFV87_18995"
     CDS             complement(946..1146)
                     /locus_tag="VFV87_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105917.1"
                     /translation="MKLLIRLTKGEEARALPILLRRSPGVVLQDRTYLSSGDAVEALR
                     QAGIRFGEISGESSSPGLIRLR"
     gene            complement(1235..1501)
                     /locus_tag="VFV87_19000"
     CDS             complement(1235..1501)
                     /locus_tag="VFV87_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5105918.1"
                     /translation="MDRQTLRAKLIDIFEQETWQRPESLDDDVAIREGLNLDSVDVLS
                     IALRVESEIGVSLDSGDFAKIQTVGGLLDTIQAKLAAKDRARAA"
     gene            complement(1657..>2455)
                     /locus_tag="VFV87_19005"
     CDS             complement(1657..>2455)
                     /locus_tag="VFV87_19005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX5105919.1"
                     /translation="REQLGSEGPTATIAAACASGNYAIAQARRWIELGLVDVCLAGGV
                     ESITPMCRANFYNLRALSRRVDDARRASRPFDLDRDGFVIGEGAAMLVLESAEHARRR
                     GARAYAEVAGFGATSDAFHMIIPSSDPAPASLAMKRALADAQVSPSEVNYVNAHATAT
                     PVGDKGEARALHAAFGDDVAHVPVSSTKSMTGHLLSAAAAVESLACVVAIQRQAIPPT
                     INLDQPDPECNLRLVAHQAVEWPVKVAMSNSFGFGGSNTTLVLRKVA"
BASE COUNT          490 a          758 c          785 g          422 t
ORIGIN      
        1 attgctgaag agctgcgcga ggaactgcac ggggaggtcg gtcttttgcg tgctccgcgt
       61 cacgagcaag ccggcggcca gcgagatgag gaacgccggc acctggctga ccagtccgtc
      121 gccgatcgtc agcttggtga agacctcggc ggcttcggtg aacgtcattc cctcctgaac
      181 gacgccgatc acgaagccgc ccaggatgtt gatcagcgtg ataacgatgc ccgcgatagc
      241 gtcgccgcgg acgaacttgc tggcaccgtc catggcccca tagaaatcag cttgctgcgt
      301 gatttcttcg cgccgtcgct gggcctcctt ttcgtcgatg actcccgcgt tcaggtcggc
      361 atcgatcgcc atctgccggc ccggcatgcc gtccaaggca aagcgggcgg cgacttcgct
      421 gattcgcgtg gcgcccttgg tgatgaccac gaactgaatc acgacgatga tcacgaagat
      481 gatgatcccg acgacgatct ggtcgccggc gacgaattcg ccaaagctct tgatgacgcc
      541 gccggcggcc agcaggccgt cctggccggc gtgcgtcaga atcagccgcg tgctggcgac
      601 gttcagcacc aggcggcaga gagtcgtcgc cagcaacatc gacgggaaga tgttgaattc
      661 cagcggcgtg cggacataga tcgtggtgag gagcacgatc accgacagcg tgacgttcgc
      721 cgccaggaag agatcgagca tgaacgacgg cagcgggacg atgatcacga gcacgctcgc
      781 gatcagtccc agggggagca cgaaatcttg ccagcgggag agtcgcgcca tacgtcatgg
      841 ccgccgcgca ccggcagcgg caaacggaaa atccgcactc gataaggggt agagaagagg
      901 gcgggagagt agcgaaaggg gcccggctgg tccagggcgg tttcatcacc gcaggcgtat
      961 gagaccgggt gaactcgatt cgccgctgat ctccccaaag cgaattccgg cctgccggag
     1021 cgcctccacg gcgtcgccgg acgagagata cgttcggtcc tgaagtacaa caccaggaga
     1081 acggcgcagg aggatcggca aggctcttgc ctcctcacct ttggtcagtc gaatcaacag
     1141 tttcatgggc aggtcacctc cgggattcga tctattgtat tccgggagga gagtccggat
     1201 aaagcaacac ttgtgcgact caacaccgcg ccgcttatgc cgcccgagcg cgatctttgg
     1261 cagccaactt ggcctggatc gtatcgagca gaccgcccac cgtctggatc ttcgcgaagt
     1321 ctccgctatc gagcgaaacg ccgatctcgg attccacgcg cagcgcaatg ctgagtacat
     1381 cgacggagtc gagattcagc ccctcgcgga tcgccacgtc gtcgtcgaga gactcaggcc
     1441 gctgccaggt ttcctgctca aagatgtcaa tcagcttggc gcgaagggtt tggcgatcca
     1501 tgcctgtgcc ttttgatggg tagcagcaaa tagtttgttt acggcagtcg cggcttaagg
     1561 gaagatgact gcttcctggc gcgaaaccgc ccgccaagcg gcggaaacga taccaaatca
     1621 ggccgctagc agaaaagacc gattgccggc gatcaatcag gcgaccttgc gcaggaccag
     1681 ggtcgtattg ctgccgccga agccgaacga attcgacatg gccactttca ctggccactc
     1741 gaccgcctgg tgagcaacaa ggcgcagatt gcactctggg tcaggctggt cgaggttgat
     1801 cgtcggcgga atcgcctgcc gctggatggc gacgacacac gcgagcgatt ccacggcagc
     1861 ggctgcgctg agcaagtggc cggtcatgct cttggtggaa ctgacgggaa catgggcgac
     1921 atcgtctcca aatgccgcgt gcagggcacg cgcctcgccc ttgtcgccca cgggcgttgc
     1981 cgtggcgtgg gcattgacat aattgacctc cgagggcgaa acctgggcgt cggccagggc
     2041 ccgcttcatt gccagcgacg ccggggccgg atcgctgctg ggaatgatca tgtgaaaggc
     2101 gtcgctcgtc gccccaaagc cggccacctc ggcatacgcg cgagctccgc ggcggcgggc
     2161 atgctcggcc gattccagga cgagcattgc cgcgccttct ccgatcacga agccgtcgcg
     2221 atccaggtcg aacggccgcg aagcccggcg ggcgtcatcc acgcgccgcg aaagggcccg
     2281 caaattgtag aaattggccc ggcacatggg cgtgatcgat tccacgccgc cagccaggca
     2341 aacatcgacc agtcccagct cgatccagcg gcgggcctgg gcaattgcgt agttcccgct
     2401 ggcgcaagcc gcagcaatcg tggcggttgg gccttcgctg cccagctgct ctcgc
//
LOCUS       DASUGB010001005         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_530153, whole genome shotgun sequence.
ACCESSION   DASUGB010001005 DASUGB010000000
VERSION     DASUGB010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530153"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFV87_19010"
     CDS             <1..682
                     /locus_tag="VFV87_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105920.1"
                     /translation="LPAAGGFTFVDSDNNGSQDLYTSRSSDGNFSWMATLVPNQDLHS
                     LTPSDVFTLSVVVCHQRPLVNFSIDTTTSDMYLYTERVAQADFTEVGGTGYGGGEMLL
                     IWPADGSTIANNATNFEIARQTLKARSGDWIMLGGFAPRLLANGAYAYDVPVFKWYRV
                     TEADHEPEYHPSEQHYAVAVSLSGQDWDTSLCGIANTPGTNAQATLMTGVVAVYEKSI
                     RLETGTDY"
     gene            897..>3499
                     /locus_tag="VFV87_19015"
     CDS             897..>3499
                     /locus_tag="VFV87_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105921.1"
                     /translation="MATRKRSSAAIQNPKSKIQNRKRRGIVLLIVVSLLALFILMGVA
                     FVIVAINYKKTAETMSLVEQRGDPSPRETELVLDQLLFDTLGRSALKYHSLLADLYSG
                     DILQGTVTDVTTAPNGYSQSNQIFTFTFSTTGTASANPHYYAGRVLTLNYGSTGKFHS
                     TRIVAYNPTTYQLSVEVNPGFIQPRASVAGPTTVVPDTFLINGAPFNGTGMGFDGITY
                     NMDAVYGHSQTYNIGMLTPADQVKDLVALLPHFNGYDLQSGSLYFNASNGMGGSPQSL
                     SLVDNGGLDEPWDAVDLHNMFLAMVMPDTATNFVTPRPVVPSFHRPELVGYWVERIMQ
                     EILVPAGYDPNTQFAQMVQIIVLPYGNDRVRLTADDPVSMTLPLAQLDRIYGIMHGCV
                     FRPMPWDHPNFSGGNQAMGANFAQMLDALSGNSLPSPPFPGAPLNPWDVDNDNDGVPD
                     SIWVDIGLPIITRPDGRRVKRLAAILIKDLGGRVDLNAHGTLAQLVGVNQSTTPVTSG
                     LAMGAIVPRGMGYGPAEVDFLNLFASDTTAYQSVLLGRYESSATGDTGVNARPGAPNA
                     AEALNIIKHHGVPNDYANLSGTPLQAFYANNPSWFASPPDVWGRGAIVIDYGGQPIWS
                     YTGTVGEATDTPYELTLNGFPSAFDSPYTVPELEQLWRYHDRDIAQLRTSGLPPYLTS
                     RLLSYLDSTNTSVFAADFPGSPGAPYQFRREAIGMNSHIPTPSHLPPPEIRTSLTVGS
                     STILDLYRWRLNNGTPSPPANIDEEMQRIVPWEFFKGQKFDINRWLGDGTDGYDGDGD
                     GAKEIPGSLNAIRDDMFEATQNEWAWEIGPANYTSPRVLAQHTNSVDVDQNGVANRFD
                     NAYARQLYARHL"
BASE COUNT          689 a         1121 c         1072 g          617 t
ORIGIN      
        1 gctccccgcg gccggcggct tcaccttcgt cgatagcgac aacaacggct cgcaggacct
       61 gtacaccagc cgcagcagcg acggcaattt ctcgtggatg gcgacgctgg ttcccaatca
      121 ggatttgcac agcctgacgc ccagcgatgt gttcacgctg tcggtggtcg tatgccacca
      181 gcggccgctg gtcaatttca gcatcgatac gacgaccagc gacatgtacc tctacaccga
      241 gcgggtggcc caggccgatt ttaccgaggt cggcggcacg ggctatggcg gcggcgagat
      301 gctgctgatc tggccggcgg atggctcgac gatcgcgaac aacgccacga atttcgaaat
      361 tgccaggcaa acgctcaagg cccgcagcgg cgactggatc atgctgggcg ggttcgcccc
      421 gcgactgctg gccaacggcg cctacgcgta tgacgttccg gtcttcaagt ggtatcgcgt
      481 gaccgaagcg gaccacgagc cggagtacca tccgtccgag cagcactacg ccgtggcggt
      541 gtcgctgtcc gggcaggact gggatacgtc gctgtgcggg atcgcgaata cgcccggcac
      601 caatgcgcag gccacgctca tgaccggcgt ggtcgccgtg tacgagaaat cgatccgcct
      661 ggagacggga accgactact gactttgcaa atcggcgcag gccgagggca attgaaaatt
      721 ggcaattgca aattgattga ggcagcaggg tgggctttgc ccaccaccag acagcagtag
      781 ggtgggctgt gcccaccacc agacagttca aacggggaga gttggtgggc acagcccacc
      841 ctacttcgga caggagagta ggtgggcaca gcccacccta cagagaggca ctcgttatgg
      901 caacgcgcaa acgcagctcc gcggcaatcc aaaatccaaa atccaaaatc caaaatcgga
      961 agcgccgcgg catcgtgctc ctgatcgtcg tgagcctgct ggctctgttc atcctgatgg
     1021 gcgtggcgtt cgtcattgtc gccatcaact ataagaagac ggcggagacg atgagcctcg
     1081 tcgagcagcg cggcgatcct tcgccccgcg agaccgagct ggtcctcgat cagctcttgt
     1141 tcgatacgct cggccgcagc gccctgaagt atcacagcct gctcgccgat ctgtacagcg
     1201 gcgacatcct gcaaggcacg gtgaccgacg tcaccacggc ccccaacggc tattcgcaga
     1261 gcaaccagat cttcacgttc acgttcagca ccacgggcac ggcgtcggcc aatccgcatt
     1321 attacgccgg gcgggtcctg acgctcaatt acggcagcac gggcaagttc cacagcacgc
     1381 ggatcgtcgc ctataacccg acgacgtatc agctttccgt cgaggtcaat cccggcttca
     1441 ttcagccacg ggcctcggtg gccgggccga cgacggtcgt cccagacacg ttcctgatca
     1501 acggggcgcc gttcaacggg acggggatgg gcttcgacgg catcacctac aacatggatg
     1561 ccgtgtatgg ccattcgcag acgtacaaca tcggaatgct cacacccgcc gaccaggtga
     1621 aggacctggt ggccctcttg ccgcacttca acgggtacga cctccagtcc gggagcttgt
     1681 acttcaatgc cagcaacggg atggggggat cgccacagag cttgagcctg gtggacaacg
     1741 gcggcctgga cgaaccgtgg gacgcggtcg atctgcacaa catgttcctg gcgatggtga
     1801 tgccagacac ggccaccaac tttgtcacac cgcgccccgt ggttccctcg tttcaccggc
     1861 cggagctggt gggctactgg gtcgagcgca tcatgcagga gattctcgtc ccggcagggt
     1921 atgacccgaa tacgcagttc gcccagatgg tgcagatcat cgtgctgcct tacggaaacg
     1981 accgcgtgcg gttaacggcc gacgatccgg tcagcatgac gctgccgctg gcgcagctcg
     2041 accggattta cgggatcatg cacggctgcg ttttccgccc gatgccgtgg gaccatccga
     2101 acttcagcgg cggcaatcag gccatggggg ccaactttgc gcagatgctc gacgccttga
     2161 gcggcaattc tctgccttcg ccgccattcc ctggtgcacc gctgaatccc tgggacgtcg
     2221 ataatgacaa cgacggcgtg cccgacagca tctgggtcga tatcggcctg ccgatcatta
     2281 cccggcccga cggccgtcgc gtgaaacgcc tggcggcgat tttgatcaag gacctggggg
     2341 ggcgggtcga tctcaacgcg cacgggactt tggcgcaact ggtgggcgtc aaccaatcga
     2401 cgacaccggt tacgtctggc ctggcgatgg gggccatcgt gccgcggggc atgggttatg
     2461 gtccggcgga ggtcgatttc ctgaatctat tcgcgagcga tacgaccgcc taccagagcg
     2521 tcttgctggg gcggtatgaa tccagcgcga cgggcgacac cggcgttaac gcgcggccgg
     2581 gcgctccgaa tgcggccgag gcgctgaaca tcatcaagca ccacggcgtg ccgaacgatt
     2641 atgccaatct gagcgggacg ccgctgcagg cgttttatgc gaacaatcct tcgtggtttg
     2701 ccagcccgcc cgatgtctgg ggccgcgggg cgatcgtcat cgattacggc ggccagccaa
     2761 tctggagtta caccggcacg gtgggcgaag cgaccgacac gccctacgag ctgacgctga
     2821 acggctttcc cagcgccttc gattctccct acaccgtccc ggaattggag cagctctggc
     2881 ggtatcacga ccgggacatc gcgcagctcc gcaccagcgg cttgccccct tacctgacca
     2941 gccggctgct ctcgtatctc gactccacga acacgtccgt atttgccgct gacttccccg
     3001 gctcgccggg cgcgccgtat cagttccggc gggaagcgat cgggatgaac tcgcatatcc
     3061 cgacgccctc gcacctgccg ccgccagaaa ttcgcacctc gctgacggtc ggttcctcga
     3121 cgattctgga cctgtaccgc tggcgattga ataatggcac gccgtcaccg cccgccaata
     3181 tcgatgaaga aatgcagcgg attgtgccct gggaattctt caagggacag aagttcgaca
     3241 tcaaccgctg gctcggcgac gggaccgatg gctatgacgg cgacggcgac ggggcgaagg
     3301 agattcccgg cagcctgaac gccattcgcg acgacatgtt cgaagcgacg caaaacgaat
     3361 gggcctggga gatcggcccg gccaattaca ccagcccgcg ggtcctggcg cagcacacca
     3421 actcggtcga tgtcgatcaa aacggcgtcg ccaaccggtt cgacaacgcc tatgcccgcc
     3481 agctctatgc ccggcacct
//
LOCUS       DASUGB010001006         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_389989, whole genome shotgun sequence.
ACCESSION   DASUGB010001006 DASUGB010000000
VERSION     DASUGB010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389989"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VFV87_19020"
     CDS             complement(<1..1021)
                     /locus_tag="VFV87_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5105922.1"
                     /translation="MPKDFLAGAKDEYDVVVIGSGLAGMTAANILGRAGHRVLLVEQH
                     YKLGGMATWFKRPGGHIFDISLHGFPHGMIKSCRRYWTEQIADSIVPLRAIRFDNPMF
                     SLTTSYNREDFTKLLTTTFGVPAQTVAAFFDTARGMNFYDDQRMTTRELFDKFFPGRE
                     DVIRLLMEPITYANGSTLEDPAITYGIVFSNFMSKGVYTFEGGTDRLIELMHADLVKS
                     GVDVRIKCDAQRIVVESGRVKGVVVNGRTIKCRAVVSNSNLKHTILGLVGAEHLDPQF
                     VEDARAVRLNNSSTQVYIALKPDERIDESMGDLFFTSTAPLFRTDLLLSKNVTSRTYS
                     FYYPRT"
     gene            complement(1130..>2214)
                     /locus_tag="VFV87_19025"
     CDS             complement(1130..>2214)
                     /locus_tag="VFV87_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene dehydrogenase"
                     /protein_id="HEX5105923.1"
                     /translation="FNNDIGLLESEIARAFPRQKDNFQRLLGQILDYDDLSQDVFDLS
                     ARQVLARTIDDPLLLEMLLCPLMWYGNAREDDMDWGQFCIMFRAIYLEGMGRPHAGIR
                     LILKNLVRRFRALGGELKLRSGVSRIVMDGQRATGVVLDSGEELSASRILSSAGAVET
                     LRLCDSLGETVPIRAGQLSFVESLSVLDRPPKALGCGRTIVFFNDSPQFHWRRPEQEF
                     CDVRTGVICSPNNFAYAPGEGELDDNLIRITALANFDRWQALPEDGYRLEKLRWYDRL
                     AESAVRFVPDFRSRVVDTDMFTPTTIRRFTGHANGAIYGAPHKQLDGTTSVQNLFLCG
                     TDQGYLGIVGSLVSGVSMANRHCLPV"
BASE COUNT          406 a          664 c          732 g          412 t
ORIGIN      
        1 tggtgcgcgg gtaatagaac gaataggtgc ggctcgtgac attcttgctg aggagcaagt
       61 cggtgcgaaa caacggcgcg gtcgaggtga agaacaaatc ccccatgctc tcgtcgatcc
      121 gctcgtcggg cttaagcgcg atatagacct gagtcgagct gttgttgagg cgcacggccc
      181 gcgcgtcctc gacgaattgt ggatcgagat gctccgctcc gaccaggccc agaatcgtgt
      241 gcttgaggtt cgaattgctc accacggccc ggcatttgat cgtccggccg ttgacgacga
      301 cccccttcac gcggccgctt tcgacgacga tccgctgcgc gtcgcacttg atccgcacat
      361 caacgccact tttcacgagg tccgcgtgca tcaactcaat cagccggtcc gtgcccccct
      421 cgaacgtata gacccccttg ctcatgaagt tcgagaacac gatgccgtag gtgatcgccg
      481 ggtcttcgag cgtcgagccg ttggcgtagg tgattggctc catgaggagg cggattacgt
      541 cctcgcggcc ggggaaaaac ttgtcaaaca gctcccgcgt cgtcatccgc tggtcgtcat
      601 agaagttcat gccgcgggcc gtatcgaaga acgcggcgac cgtctgggcg ggcacaccaa
      661 atgtcgtggt cagcagcttg gtgaaatctt cgcggttgta gctcgtggtg agcgagaaca
      721 tcgggttgtc gaagcggatg gcccgcagcg gcacgatcga atcggcgatt tgctcggtcc
      781 aataccgccg gcagctcttg atcatcccgt ggggaaagcc gtgcagcgag atgtcgaaaa
      841 tgtgcccgcc ggggcgcttg aaccaggtgg ccatgccccc cagcttgtaa tgctgttcga
      901 cgaggagcac gcggtggccg gcccggccga ggatatttgc cgcggtcatg ccggccagcc
      961 cgctgccgat cacgacgacg tcgtattcgt ctttcgcacc ggcgaggaag tccttgggca
     1021 tactggcaag acaagtaggg gattgatcgt atccagtact tagtactgag tactttccgc
     1081 gcctccgtca aggcggggct ggagtgagga ctgcgccggt cggcggccct cataccggca
     1141 aacagtgccg gttggccatc gagacgccgc tgaccagtga gccaacgatg cccaggtacc
     1201 cctggtcggt gccgcagagg aaaaggttct gtacgctcgt cgtgccgtcg agctgcttgt
     1261 gcggcgcgcc gtagatcgcc ccattggcgt gccccgtgaa ccggcggatc gtcgtcggcg
     1321 tgaacatgtc ggtatcgacg acgcggctgc gaaaatcggg aacgaaccgc acggcggatt
     1381 cggccagccg gtcgtaccag cggagctttt ccaggcgata gccgtcctcg ggcagggcct
     1441 gccagcggtc gaagttggcc agggcggtga tgcggatcaa gttgtcgtcg agctcgcctt
     1501 ctcctggagc gtacgcgaaa ttgttgggcg agcagatcac gcccgtccgc acgtcgcaga
     1561 actcctgctc gggccgccgc cagtggaact gcggcgagtc gttgaaaaac acgatcgtcc
     1621 ggccgcagcc gagcgctttg ggcggccggt cgagcaccga aagcgattcg acgaacgaca
     1681 gttgcccggc ccggatgggg acggtttcgc ccaggctgtc gcacagccgt agggtctcga
     1741 ccgcgccggc ggacgacagg atgcggctgg cgctgagctc ttcgccgcta tcgaggacga
     1801 cgccggtggc ccgctggccg tccatgacaa tgcgcgagac gcccgagcgg agcttgagct
     1861 cgccccccag tgcgcggaat cgccgcacga gattcttgag gatcaggcga atgccggcgt
     1921 gggggcggcc catgccttcc aaatagatgg cccggaacat gatgcagaac tgcccccagt
     1981 ccatgtcgtc ttcgcgggcg ttgccgtacc acatgagcgg gcagagcagc atttccagga
     2041 gcaaggggtc gtcgattgtg cgggccagga cctggcgggc ggacaggtcg aacacgtcct
     2101 ggctgaggtc gtcataatcg agaatctggc cgagcagccg ctggaaattg tccttttgcc
     2161 gcgggaacgc gcgggcgatt tcgctttcca agaggccgat gtcgttgttg aaat
//
LOCUS       DASUGB010001007         4899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1917664, whole genome shotgun sequence.
ACCESSION   DASUGB010001007 DASUGB010000000
VERSION     DASUGB010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917664"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFV87_19030"
     CDS             complement(<1..869)
                     /locus_tag="VFV87_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX5105924.1"
                     /translation="MPTVSRYAPYTAALQFAERQVKALIEANPDYFPMYTVGGRWRHE
                     GETWTHWTDGFLGGMMWQFHLRQVANGKHPHPGPFPEGVGDYWRERAEHYSKLLEHRQ
                     HDRNVHDLGFIFLSTYLPWYELTGELHLHDVLIQAGQTLAMRFKVKGQYLRSFVSDDS
                     LFIDIMMNVPIIFYAAIETEDRELARIATAHCRTTRDKLVRQDGSTAHEGIFDLESGK
                     FLREDTHQGLAPDSAWARGLAWSLYGYSKCYALTGNREFLEVAERNAKYWIDHLPADG
                     VPYWDFHADLSKPA"
     gene            complement(923..2551)
                     /locus_tag="VFV87_19035"
     CDS             complement(923..2551)
                     /locus_tag="VFV87_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5105925.1"
                     /translation="MTSTESTNSYHSAPASITADPDRFTAGQPIGERFRILTPLGKGG
                     MGEVFQAEDLLLGQSVALKFLPQRLTLAPDRLQRLRSEVKTARQVAHPNVCRVYDLDE
                     AAGQFFLTMEFIDGQNLASVLKQSGALTEERGVEIFRQLCLGLGAIHDQRVLHRDLKP
                     SNVMLDSRGQVKITDFGLAAYAELLQPHELFDGTPAYQSPEQRAGRAVTARSDLFALG
                     LIMYEVFAGSRPPTGDAQVALPDANSAQPADSSPRRLSTVSPAVEQIILKCLSREPAE
                     RPASAYEVLAALPEATMEGSLNPRLEIARETVKRHRLEESRQDQYWSAYLESYDRLLM
                     EFPPFMRLIDDLIAVVPPGRQAVVDLGAGTGCATAALLAAGHAVTAVESSAAMIDRLR
                     YLAKKMRGMSLRIVPSKVDDLRALADSEFDAAVMLNVLYAVTDPLACLRGVHRILKPR
                     GVLAFSTTHAEISLDALLTSLKDWLEATGQYEVRSNDYQLIWDLNKRIETTIAKRHTR
                     DEYRAWTRAAGFEIIHDVPSTYEGAVMMIHARKI"
     gene            2764..3249
                     /locus_tag="VFV87_19040"
     CDS             2764..3249
                     /locus_tag="VFV87_19040"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX5105926.1"
                     /translation="MRYSPLLHIVLHQPEIPPNTGNIGRSCVAIGAKLWLVKPLGFEI
                     SEKQVRRAGLDYWPRLEWEAVEDWIELTTKLAEPLAHGRGWFFTKTASHNYTDVAYQK
                     GDILVFGSESAGLPAEILAQYQSHAVRVPMREQVRSLNLSAAAAVAMYEAVRQIGADI
                     R"
     gene            complement(3283..3633)
                     /locus_tag="VFV87_19045"
     CDS             complement(3283..3633)
                     /locus_tag="VFV87_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5105927.1"
                     /translation="MRMVLALIQPTKLKAVQEALHRIGVERMTVCDALGYGQQLGRTV
                     AAAADGGKATLLRKVALEIAVNEDFLDKTVETIAQIAKSGPAGAIGDGKIFVLPVEQV
                     VRLSDTVCGPEAVS"
     gene            complement(3683..4570)
                     /locus_tag="VFV87_19050"
     CDS             complement(3683..4570)
                     /locus_tag="VFV87_19050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5105928.1"
                     /translation="MPRPGALPYLWMLCGAASFSVMAIMASGLKEECDWQWIAIARTG
                     LAMIFSVMLAVGGGAKLVFLRPAKLWMRSVAGSLSLVCGFYAMTHYPISEVLTLTNMF
                     PLWVAVLSLPLLGEWPPLDVWPSMLVGLMGIVLIQQHQLPGSHVAVIAAIVNSLLSAV
                     ALIGLHQVREVDPRAIVAHFSAVALTACVAALFLLPRSKPLVLDLPTAATMLGVGVSA
                     TIGQLFMTKAFAHGPPARVSVVMLSQVGFTMLLEMALWHRTFSSLTLIGIGLVVAPTA
                     WTLLRRTPAAKEVPIEEPV"
BASE COUNT          971 a         1443 c         1594 g          891 t
ORIGIN      
        1 gctggcttcg aaagatcggc atggaaatcc cagtagggaa cgccgtcagc cggcagatgg
       61 tcgatccagt atttcgcgtt ccgctcggcg acttccagga attcgcggtt gccggtgagg
      121 gcgtagcact tgctgtagcc atagagcgac caggccaggc cgcgggccca ggcgctatcg
      181 ggcgcgaggc cctgatgcgt gtcctcccgc aagaatttgc cgctttccag gtcgaatatc
      241 ccctcgtggg ccgtcgatcc gtcctgccgc acgagcttgt cgcgcgtcgt gcggcagtgg
      301 gccgtggcga tgcgggccag ctcacggtcc tcggtctcaa tcgccgcata gaagatgatc
      361 gggacgttca tcatgatgtc gatgaacagc gaatcgtcgc tgacgaacga gcggagatac
      421 tggcccttca ccttgaaccg cattgccagc gtctggccgg cctggatcag cacgtcgtgc
      481 aaatgcagtt cgcccgtcaa ctcgtaccac ggtaaatacg tgctgagaaa aatgaacccc
      541 aggtcgtgga cgttgcggtc gtgttggcgg tgttcaagga gcttggaata gtgctccgcc
      601 cgctcgcgcc agtagtctcc gactccctct gggaaagggc cgggatgagg gtgcttgcca
      661 tttgcaacct gccgcagatg aaactgccac atcattcccc cgaggaatcc atccgtccaa
      721 tgcgtccacg tctcgccttc gtgccgccac ctgccgccga cggtatacat cgggaaatag
      781 tcgggattgg cttcaatcag cgctttcacc tgccgctcag cgaactgaag ggcggcggtg
      841 tagggggcgt agcgagaaac agtgggcatc ggcggtgacc ggttgaagga tgcaggatac
      901 ttgccgcttg tcctggcgac gttcagatct tgcgggcatg gatcatcatg accgcgcctt
      961 cgtacgtgct cggcacgtca tggatgatct caaacccggc agctcgggtc caggcgcgat
     1021 attcatcacg ggtgtggcgc ttggcaatcg tcgtttcgat ccgcttgtta agatcccaaa
     1081 tgagctgata gtcgttcgat cggacttcgt actggccggt cgcttcgagc cagtccttca
     1141 gacttgtcag gagggcatcg aggctaatct cggcgtgcgt ggtggaaaat gccagcacac
     1201 cgcggggctt gagaatccga tgcacaccgc gcaagcacgc caacggatct gtcaccgcgt
     1261 agagaacgtt cagcatcacg gcggcgtcga actcgctgtc ggccagcgca cggagatcgt
     1321 cgaccttcga cggcacgatc ctcaatgaca tgccccgcat cttcttggcg aggtagcgga
     1381 ggcgatcgat cattgctgcg ctggattcga cggctgtgac ggcgtgtccg gctgccaaca
     1441 gggcggccgt ggcgcagccg gttcctgccc ccaaatcgac aactgcttgt cggcctggcg
     1501 gcacgacagc aatcaagtca tcaatcaacc gcataaaggg tggaaactcc atgagcaacc
     1561 ggtcatacga ttccaaataa gcactccaat actggtcctg ccgcgactct tccaggcggt
     1621 ggcgcttgac ggtttcgcgc gcgatttcca gccgtgggtt caagctccct tccatcgtcg
     1681 cttcgggcag tgcggctaat acttcgtaag cgctcgccgg acgttcggca ggttcacgcg
     1741 agaggcactt gagaatgatc tgctcaacgg ctggacttac tgttgagagg cgacgcggcg
     1801 aggaatctgc tggttgtgcc gaattggcat cggggagtgc gacctgcgca tcgccggtcg
     1861 gcggccggct gccggcgaag acctcgtaca tgatcaatcc gagagcaaac aagtcgctgc
     1921 gtgcggtcac cgcgcgaccg gcgcgctgtt cgggcgattg ataggcggga gtgccatcga
     1981 acaactcgtg gggttggagc aactcggcat acgctgcaag gccaaaatcg gtgatcttca
     2041 cctgtccgcg actgtccagc atgacattcg aaggtttcag atcgcgatgc aggactctct
     2101 ggtcatgaat cgcgcccagg cccaggcaga gctgacgaaa gatctcgacg cctcgctcct
     2161 cggttagggc gccgctctgc ttgaggaccg atgccaggtt ttggccgtcg atgaactcca
     2221 tcgtcagaaa gaactgacca gcggcctcgt ccaggtcgta aacgcgacag acattgggat
     2281 gcgctacctg gcgggcggtc ttgacctcgc tccgcaggcg ctgcagtcga tccggtgcga
     2341 gcgtcaaccg ctgcggcagg aatttgagag cgacggactg tcctagcaac agatcttcgg
     2401 cctgaaagac ttcgcccatt cctccctttc cgagcggcgt caggatgcga aatcgctcac
     2461 cgataggttg gccggccgta aagcggtccg gatcggcggt aatactggcg ggcgcggaat
     2521 ggtacgagtt ggtggactcc gtcgaagtca ccccgcctcc tgtgctgcag cgagggcaaa
     2581 gtgagtcgct gctgaacaac tcgcttccgc aggacgggca gcgtcggaac tgagtcggta
     2641 agtcaggcat ggcattgacg gcaagtcgat gctcgcgata agccctaagg ggccatgcac
     2701 gccatgcccc acattgtagg cgcttaacgc aaaaggacgc cactagtagc ttcgaggtga
     2761 cgcgtgaggt attcgccatt actacacatc gttctccatc agccggagat tccgccgaac
     2821 acggggaaca tcgggcgaag ctgcgtcgcg atcggggcga agctgtggct ggtgaagccg
     2881 ttggggtttg aaatcagcga gaagcaggtc cggcgggccg ggctcgatta ctggccgcgc
     2941 ttggagtggg aggcggtcga ggattggatt gaactgacga cgaaactcgc cgagccgctg
     3001 gcccatggcc gcggctggtt cttcaccaag accgccagcc acaattacac cgatgtcgcc
     3061 taccaaaaag gggacattct ggtttttggc agcgaatcgg ctgggctgcc ggcggaaatc
     3121 ctggctcagt accagagcca cgccgtgcgc gtgccgatgc gggagcaagt gcggagcctg
     3181 aatctctccg ccgcagccgc ggtcgccatg tatgaggcgg tgcggcagat tggggcggac
     3241 atccggtgaa tcgaggaacc gcgaccactg gtcgcggctt tgtcaggata ccgcttccgg
     3301 gccgcagacg gtgtcggaga ggcggacaac ctgttcgaca ggcaggacaa agatcttgcc
     3361 gtcgccgatt gcgccagcag ggccgctctt ggcgatctgg gcgatcgtct cgacggtctt
     3421 gtcgagaaag tcctcgttga ccgcgatttc cagagccact ttgcgcagga gagtcgcctt
     3481 gccgccgtcg gccgcggcag cgaccgttcg ccccaattgc tgcccgtagc cgagggcgtc
     3541 gcaaacggtc attcgctcca cgccgatgcg gtggagcgcc tcctgcactg ctttgagttt
     3601 ggtgggctga atcagggcca ggaccatgcg catcggaaat ctccctgggg cggacgggag
     3661 aggcggtttc aggatagcag cgtcagaccg gctcttctat gggcacttcc ttggcggccg
     3721 gcgtgcggcg gaggagcgtc caggcggtcg gggcgacgac caggccaatg ccgatgagcg
     3781 tcaggctgct gaacgtgcgg tgccaaaggg ccatctccag gagcatcgta aagccgacct
     3841 gcgagagcat cacgaccgaa acgcgggccg gcgggccgtg ggcgaaggcc ttggtcatga
     3901 agagctggcc gatcgtcgcc gagaccccca cgccgagcat cgtggcggct gtcggcagat
     3961 cgagcaccag cggtttgctg cgaggcaaga gaaacagcgc cgcgacacag gcggtcaggg
     4021 ccacggccga aaaatgggcc acaatcgccc gcggatcaac ctcccgcacc tggtgcaggc
     4081 cgatcagggc gaccgcgctg agcaacgagt tgacgatggc ggcgatcacg gcgacgtggc
     4141 tgcccggaag ctgatgctgc tggatcagca cgatccccat cagcccgacg agcatcgacg
     4201 gccagacatc cagcggcggc cactcgccca aaagtggcag cgagagcacg gccacccaga
     4261 gcggaaacat gttcgtcagc gtgagcacct ccgagatcgg atagtgcgtc atcgcataaa
     4321 agccgcaaac caggctgagg ctgccggcga cgcttcgcat ccagagtttc gcggggcgga
     4381 ggaagacgag ttttgcgccg ccgccgacgg cgagcatcac ggagaagatc atcgccagcc
     4441 cggtgcgggc gatggcgatc cattgccagt cgcactcttc cttgaggccc gacgccatga
     4501 tcgccatgac cgagaacgag gcggctccgc agagcatcca caaatagggc aaagcgccgg
     4561 ggcggggcat ggaagtgggc gggcagggtg cggcgggcag acctcaagtg tattcacgag
     4621 cggctgccga atgaagggca ggaggtggcg ctgccgcgtc gggttcgcgc cgtgaagagc
     4681 aattgctcga cgcggcatgg gccgaaacac acaatgcgag tgcccacccg atcgagtgca
     4741 aattgcaaat tgaacattgc aaattgcaaa ttggaggcgg cagctcgtct gcttcaattt
     4801 gcaatttgca tttcgcaatt tgcagtcagc gtgcgcgcac ggcgtgattt ggccggacgg
     4861 acgggttgga aacccgtcct acatacgaca gacgggttg
//
LOCUS       DASUGB010001008         5425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_667595, whole genome shotgun sequence.
ACCESSION   DASUGB010001008 DASUGB010000000
VERSION     DASUGB010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667595"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..2611)
                     /locus_tag="VFV87_19055"
     CDS             complement(122..2611)
                     /locus_tag="VFV87_19055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105929.1"
                     /translation="MYLIRLSTLLALAALLVPAAIAQAAPAEDAYAAAAGLYSAQQWE
                     QAAEALGQFLAQYPDHERATVARFYRGEALAQLGRHESAASLHGEFLTLAPQHALARV
                     AAFRVGECLLLAGKGDEAKTRLEEFRRSHPQDELNAFALPYLGEIALAAGKTAEAEDL
                     YVQALSDYPSGLMAAESRFGLASCRQKSGQAADAIALYQQVATDTNHALADDARLRIG
                     LVHYDAQQFADAEESFSAFRTTFAESQLAPQARYWLGMSQQAQGRAAEAAATLAAAAA
                     EFEQAEQAPALHAAAGDAFRHAGDTARADAHFQRLIADWPQSEWADDALLGRAELALA
                     AGEADRAATLAAELAEKYPQSPHAGLAGIIRARSLLAEEQFAAAEELLQPLVVEAKPE
                     AATDGPPAASAANHQLAYLLALAQIGQKKHEAALASLDKIGDLPEGDSLAAPVAEARC
                     SALLGLERFDDAAALLQQRIESDSGGEAEAAELSVQLVVALARSGKLGQAVAQIQNLP
                     QNAVQSQEVAAVVLLAAEEAYKSNNLPTAESLFTLAAGEGVPAAERSLALSGLAWTQY
                     RRSGKQASAATFDRLLRDFPSSPLAAEAALVRAKSLEDVKQYDAAISTYRLIIDKYAT
                     SPWLTHALWGAARLHDQLNQDREAAELLTRLVNDYPDFTHRDAALYQLGWVHADLQGH
                     ASSEAAHAQLLKEYPESEFWADAAYHLAEQAARRQENSRAIELTDQLLAHKSGAGVRE
                     NALYLRGQLAAAEGKWDEVARYMGDHALQYPASAMNLSARYWHAEAAFRIAPKARAGG
                     LSANGQIAVLPNPPFAFGERPPCQGVGGGRA"
     gene            complement(2792..3679)
                     /locus_tag="VFV87_19060"
     CDS             complement(2792..3679)
                     /locus_tag="VFV87_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105930.1"
                     /translation="MPTSIALPPQLTIRLVKWSDLAWLPAGLVLLIAGGVAIAVRGQF
                     AEVVALGPLGGIAIAIGLIATGIGLFWAWRASQAMNVQFGDEIQLARLFGSRVRVPWD
                     EVAEVSFHRSERQYEQHSTDGMWLVPIPGVGALTVTSVSTKTRWLVERYVELIGPNGR
                     PIVRIGRGATDELRFTLWYERHQLRQIIQSLNGRIEWSAQGLSHVSLAGTNVGDAVFV
                     PIKQRLIRIRELQAIDLSRTKVTATALDGLEECESLASVECGQTQIPQSARDDLGRKL
                     ASRRANTGFGFLQPQRNAQ"
     gene            complement(3731..>5425)
                     /locus_tag="VFV87_19065"
     CDS             complement(3731..>5425)
                     /locus_tag="VFV87_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEX5105931.1"
                     /translation="GLRPDRATLSYCQRAREAYPGVPVIAGGVESSLRRLAHYDYWSD
                     KVRRSILLDCKANLLVYGMGEEAILEIAQRLASGETVKDLRSMRGVAYALGASEAVPA
                     WGMQNSEFRMQKAEETNSEFCILNSELANSITLPSYEQVLADKPTFAEATRLIHNETN
                     PYNAKRLVQWHDRQAVVANPPPFPISQASMDAIYTLPYTRLPHPSYTEPIPAFEMIKD
                     SVTIMRGCFGGCTFCSITAHQGRIIQSRSKESVLGEIQEMAADPKFKGTISDIGGPTA
                     NMYEMKCTKPEVEAVCRRQSCVHPTVCKLLGTDHGPLVELMKDARETPGIKKVLVASG
                     IRMDLARRSPEYMRELARHHVGGHLKVAPEHTDPDVLMKMRKPSNDDFELFAEEFNRE
                     SKKAGKKQYIVPYFIASHPASDLDAMIHLAVFLKQNGYKPDQVQDFIPAPFDVATAMY
                     YTGLDPFTKKPVHIARHLRDRKLQRALMQFFKPENYFEVREALQQAKRTDLIGDGCDS
                     LIASRPPKEALQRRRQDANTRFAGNYVHTPPGTGAGKRRPVKQSRRNPGRGYRPKD"
BASE COUNT          876 a         1689 c         1834 g         1026 t
ORIGIN      
        1 atgatgcgcc actgcgaggc gcggtagaac ttctcctgct cgggcgtgag gctctcggcc
       61 gccgcgggct cgaagggcgc gcgcgagacg aagtgttcga gcggcgcttc gcgtacgtcg
      121 ctcatgcccg ccctccgccc accccctgac aagggggtcg ttcgccgaag gcgaacgggg
      181 ggtttggcag cacggcgatt tgcccgtttg cagacaaacc ccccgctcgc gccttcggcg
      241 cgatgcggaa ggccgcttcc gcgtgccagt atcgggcaga aagattcatt gcactggccg
      301 gatactgcag ggcgtgatcg cccatgtagc gggcgacttc gtcccacttg ccctccgccg
      361 ccgcgagctg gccgcggagg tacagcgcgt tctcgcgtac gcccgcgccg ctcttgtgcg
      421 cgagcaactg gtcggtcagc tcgatggccc ggctattttc ctggcggcgg gcggcctgct
      481 cggcgaggtg ataggcggcg tcggcccaga actcgctctc aggatattcc ttgaggagct
      541 gcgcgtgggc ggcttccgac gaggcgtggc cctgcaaatc cgcgtggacc cagccgagct
      601 ggtacagagc tgcgtcgcgg tgcgtgaaat cgggataatc gttcaccagc cgcgtcagta
      661 gctcagcggc ttcgcgatcc tggttgagct gatcgtggag gcgggcagcg ccccagaggg
      721 cgtgtgtgag ccagggggac gtggcgtact tgtcgatgat caggcgatag gtgctgatcg
      781 ccgcgtcgta ctgcttcacg tcctcgagcg acttcgcccg cacgagggcc gcttcggcgg
      841 cgagcgggct gctgggaaaa tctcgcagca gacggtcgaa tgttgcggcg gaggcttgct
      901 tgcctgagcg gcggtattgc gtccaggcca gcccggaaag ggccaggctg cgctcggctg
      961 cgggaacgcc ttctccagcc gcgagcgtga agagcgattc cgcggtcggc agattgttgg
     1021 atttgtaagc ctcctccgcc gcgagcagca cgacagccgc gacttcctgg ctctgcacgg
     1081 cgttttgcgg cagattctgg atttgcgcga cggcctggcc gagcttgccc gagcgggcca
     1141 gcgccaccac gagctgcacg ctcagctcgg ccgcttccgc ttcgccgcca gaatcggatt
     1201 cgatgcgctg ttgcaagagc gcggccgcgt catcgaatcg ctccagcccc agcagggccg
     1261 agcagcgggc ttcggctacg ggagcggcga gagaatcgcc ttctggcaaa tcgccgatct
     1321 tatcgagcga cgcgagggcc gcctcgtgct tcttctggcc gatttgagcc agcgcgagca
     1381 agtacgcgag ctgatgattg gcggcggaag ctgccggcgg gccgtctgtg gccgcttctg
     1441 gcttggcttc gaccacgagc ggctgcagga gttcttcggc ggctgcgaac tgctcttcgg
     1501 cgagcaagct gcgggcgcga atgatgcctg ctaagccggc gtgtgggctt tgcgggtact
     1561 tctcggccaa ttcggcggca agcgtcgcgg cacggtccgc ttcgcccgcg gccagggcca
     1621 gctcggcacg gccgagcaag gcgtcgtcgg cccattccga ctgcggccaa tcggcaatca
     1681 gccgctggaa atgggcgtcg gcgcgagccg tgtcgcccgc atggcggaag gcatcgccgg
     1741 cggcagcgtg cagggcaggg gcttgctcgg cttgctcgaa ttccgctgcc gcggcggcca
     1801 gcgttgcggc ggcttcggcg gcgcggccct gggcttgctg gctcatgccg agccagtacc
     1861 gggcctgcgg ggccaattgg ctttcggcaa acgtcgtgcg aaacgccgag aacgactctt
     1921 ccgcgtcggc gaactgctgg gcgtcgtaat gcaccaggcc gatccgcagg cgggcgtcgt
     1981 cggccagcgc gtgattcgtg tcggtcgcga cctgctgata gagggcgatc gcgtcggcag
     2041 cctggccgct cttctgccgg cagctcgcca ggccgaaacg cgactcggcg gccatcaatc
     2101 cgctcggata gtcgctgagc gcttggacat acaagtcttc cgcctccgcc gtcttgccgg
     2161 ccgccaaggc gatttcgccc aggtaaggca gcgcgaaggc gttgagctcg tcctgcggat
     2221 ggctccggcg gaactcttcc agccgggtct tcgcctcgtc gcctttgccc gccagcagca
     2281 ggcactcgcc gacgcgaaac gctgccacgc gggccaaggc atgctgcggc gcgagcgtca
     2341 gaaactcccc gtgcaagctg gcggccgatt cgtgccggcc gagctgcgcg agggcctcac
     2401 cgcgatagaa ccgggcgacg gtcgcccgct cgtggtccgg atactgagcg agaaactggc
     2461 cgagcgcctc cgcggcctgc tcccattgct gggccgaata aagcccggct gcggcggcat
     2521 aggcatcctc tgccggcgca gcctgagcga tggcggctgg caccagaagg gccgcaagcg
     2581 caagcagcgt cgaaagtcga ataaggtaca tcctgtacct gcgtggcaat cctttgccgg
     2641 tttttgtaga tgaagagcgg attcaacctg cgcaggattc cgcacagcca tccgcttaac
     2701 cctcacctcg atggcatcgg ccataggcga cttcccaaat cacgcccaga accgcaatcg
     2761 caacagattg cctaaatcgc ccagtatctg cttattgtgc attcctttgt ggctgaagaa
     2821 agccgaatcc ggtgttggcg cggcgggacg ctaacttgcg gcccagatcg tcgcgagctg
     2881 attgcggaat ctgagtttgg ccgcactcaa cgcttgcgag cgattcgcac tcttccagtc
     2941 cgtccagcgc tgtggcggtg accttcgttc gcgagagatc gatagcttgc aattcacgga
     3001 tccgtatgag tcgctgcttg atgggcacga acacggcatc gccgacgttc gtcccggcga
     3061 gcgaaacatg cgacaggcct tgcgcgctcc attcgatcct gccgttcagg ctctgaatga
     3121 tctgtcgcag ttgatggcgc tcgtaccaca aagtgaaccg caattcgtcg gttgcgccgc
     3181 gtccgattcg cacgatcggc cgcccgttgg gacctatcaa ttcgacgtat cgctccacga
     3241 gccagcgcgt cttcgtcgag acggaagtca ccgtcagggc tccgacaccg gggatcggca
     3301 cgagccacat tccatccgta ctgtgctgct catattgccg ttcagagcgg tgaaacgaca
     3361 cctccgccac ttcgtcccac gggacgcgga cccgcgatcc aaagaggcgc gcaagttgaa
     3421 tctcgtcgcc aaattgcacg ttcatggcct gcgacgcgcg ccaggcccaa aacagtccga
     3481 taccggtcgc aatcagcccg attgcgattg caatgccgcc cagcgggccg agggccacga
     3541 cttcggcgaa ttggccgcga acggcgatgg ccacaccgcc agcgataagc aatacgaggc
     3601 ccgccgggag ccacgccagg tcgctccatt ttacgagtct aatcgtcaac tgcggcggca
     3661 gcgcaatcga ggttggcatg ccgagagtct attcagtgcc gctggcaaat gccaactctt
     3721 ggtcacgttt tcagtccttc ggccgatatc cgcggccggg gttgcggcgg gattgtttga
     3781 cgggtcgtcg cttgccggca ccggtgccgg gcggcgtatg gacatagttg cctgcgaagc
     3841 gcgtgttggc atcctggcgg cggcgctgga gggcttcttt cggcgggcgg ctggcgatca
     3901 ggctgtcgca gccgtcgccg atgaggtcgg tgcgcttggc ttgctgcagg gcttcgcgga
     3961 cctcgaaata gttttcgggc ttgaagaact gcatcagggc ccgctggagc ttgcggtcgc
     4021 ggaggtggcg ggcgatgtgg accggctttt tcgtgaaggg gtcgaggccg gtgtagtaca
     4081 tggcggtcgc cacgtcgaaa ggcgcgggaa tgaagtcctg cacctgatcg ggcttgtagc
     4141 cgttctgctt gaggaagacg gccaggtgga tcatcgcgtc caggtcgctg gccggatggc
     4201 tggcgatgaa gtaggggacg atgtattgct tcttgccggc cttcttcgat tcgcgattga
     4261 actcctcggc gaacagctcg aagtcgtcat tcgagggctt acgcatcttc atcagcacgt
     4321 cgggatcggt gtgctcggga gcgactttaa ggtggccgcc gacgtgatgc cgagccagtt
     4381 cgcgcatgta ctccggcgag cggcgggcga ggtccatgcg gatgccacta gcgacgagga
     4441 ccttcttgat gccgggcgtt tcgcgggcat ccttcatcaa ctcgacgagc gggccgtgat
     4501 cggtgccgag cagcttgcag accgtcggat gcacacacga ctgccggcgg caaacggctt
     4561 cgacctccgg cttggtgcat ttcatctcgt acatgttggc ggtcgggccg ccgatgtcgc
     4621 tgatcgtgcc tttgaacttc gggtcggcgg ccatctcctg gatctcgccg agcaccgact
     4681 ccttgctccg cgactggatg attcgcccct gatgcgccgt gatcgagcag aacgtgcagc
     4741 cgccgaagca gccgcgcatg atcgtcaccg agtccttgat catctcgaac gcggggatcg
     4801 gctctgtgta cgacgggtgc ggcagccggg tgtaagggag cgtgtagatc gcgtccatcg
     4861 aggcttgcga gatcggaaac ggcggcggat tggcgacgac cgcctgccgg tcgtgccact
     4921 ggacgaggcg cttggcgttg taggggttgg tttcgttgtg gatcagccgc gtggcctcgg
     4981 cgaacgtggg tttgtcggcg aggacttgct cataggaggg gagggtgatg ctgttcgcca
     5041 attcagaatt cagaatgcag aattcagaat tggtctcctc ggctttctgc attctgaatt
     5101 ctgagttttg cattccccag gcaggcaccg cttcgctggc cccgagggca tacgccacgc
     5161 cgcgcatgct gcgaaggtcc ttgaccgtct cgccgctggc cagtcgctgg gcgatttcga
     5221 ggatcgcttc ctcgcccatg ccatagacga gcaggttggc cttgcagtcg agcaggatcg
     5281 agcggcggac cttgtccgac cagtagtcgt aatgcgcgag ccgccgcaag ctggattcga
     5341 cgccgccggc gatgaccggc acgccgggat acgcctcgcg ggcccgctgg caatagctga
     5401 gcgtggcccg gtcgggacgc aggcc
//
LOCUS       DASUGB010001009         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_111200, whole genome shotgun sequence.
ACCESSION   DASUGB010001009 DASUGB010000000
VERSION     DASUGB010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111200"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..470)
                     /locus_tag="VFV87_19070"
     CDS             complement(150..470)
                     /locus_tag="VFV87_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105932.1"
                     /translation="METTYRRYEILLPLKFNDGRPVPENLLADTLAEIEEKIGPLSFE
                     SQEIMGVWQHNGQTYRDKQARVFVDVPDTAENRQFFSGLKERLKSRFQQLDIWVTTHP
                     LEIL"
     gene            complement(521..1966)
                     /locus_tag="VFV87_19075"
     CDS             complement(521..1966)
                     /locus_tag="VFV87_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105933.1"
                     /translation="MLRVMAWLLIAAACMDVLAADPVELGSRRELFVDGYLIEKLDGA
                     ELRLQTPRDEGVAFALDKPWEGKFSAYTTIIPDGDKYRAYYRGLPESRADGSSRESTC
                     YAESADGKTWTKPSLGLFEVDGTKENNVILAGQPPFSHNFCPLIDRRKGVPAEERYKA
                     LAGSSRTGLVAWISPDGIRWKKLREEPVITGGAFDSQNVPLWSEAEQCYACYYRVFVD
                     RVRRIARVTSQDFATWSEPTLMGYSLQSDPSAKLPIEQLYTNQTSPYFRAPHIYVAIA
                     ARFFPGKRVLTPEQAARVGVDPGYFNDCSDGILMTTRGGDFYDRTFMEGFLKPGLGLE
                     NWTSRTNYPALNVVQTGPEEMSLYVNQNYGQPTAHIHRYSLRHDGFAALHAGYDGGEM
                     LTRPLTFNGQELELNFATSAAGSIRVEIQDAAGKPISGFTAEECLDTIGNELDRVVQW
                     KAGSDVSDLAGQPLRLRFLLKDADLFSLRFK"
     gene            complement(1994..2647)
                     /locus_tag="VFV87_19080"
     CDS             complement(1994..2647)
                     /locus_tag="VFV87_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105934.1"
                     /translation="MDTNPYKDPAPTSDPKRFGMKSRIFWFVLGFGTAWVIWSVTNYR
                     ALSPRDYTQNWPEELREDAPEWMKSARGRRVGLFTILAADDPQRASAQVYPSQPDRNP
                     SVAYEDTDADGRADSLLVTDSNRRTFSFAIADGKFTSYEYSPDVMASDSITFVDHDLH
                     GGFDLRFGPGGKHAMSVDSRWYELIPTGGHKGYVNINGKQVPAEHVNGAWRIVEQSQ"
     gene            complement(2786..3070)
                     /locus_tag="VFV87_19085"
     CDS             complement(2786..3070)
                     /locus_tag="VFV87_19085"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX5105935.1"
                     /translation="MLPRVTYVRHVRDYLLEVTFSDGTVAELDFRSRIVGRGGVFQAL
                     ENVECFAQVRVDSEAGTLAWPTGVDFCPDVLYAQATGRDVNTLGAELDVA"
     gene            complement(3087..3326)
                     /locus_tag="VFV87_19090"
     CDS             complement(3087..3326)
                     /locus_tag="VFV87_19090"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX5105936.1"
                     /translation="MRRRTLQSDESHNPPHFHAIYSGSEVEVGIRPLTVLRGSLPRRA
                     LGLVMEWAALHQQELLDNWALLNNEQPPHRIEPLD"
BASE COUNT          677 a         1052 c         1028 g          674 t
ORIGIN      
        1 cattcgccaa ctcgtgagcc agacggcaaa cgcgacgagg agcgtcacca agaacagcgt
       61 cttcagcgag aagcgccagg ggttggttga ggcagtagga ttgcggccag acatcgagta
      121 aatcgtactc gctgccgagt cgctttgcct cagaggatct ccagcgggtg cgtcgtgacc
      181 cagatgtcga gctgctggaa ccgggatttg aggcgctctt tgaggccgga gaagaactgg
      241 cggttttcgg ctgtgtcggg tacatcgaca aagacgcggg cctgcttgtc gcggtaggtc
      301 tggccgttgt gctgccagac ccccatgatc tcctggctct cgaacgacag cggaccgatc
      361 ttctcctcga tctccgccag cgtatcggcc agcagattct ccggcaccgg ccgcccgtcg
      421 ttgaacttca gcggcagcag gatttcgtac cggcgataag tcgtctccat ccctcagcct
      481 cctgctgaat cgtaggcacg cctgtgctgc aggcaacttc ctacttgaac cgcagcgaaa
      541 acaggtcggc atccttcagg aggaatcgca gccgaagggg ctggccggcg aggtcggaaa
      601 cgtcgctgcc ggccttccat tggacgacgc gatcgagctc gttgccgatc gtgtccaggc
      661 actcttcggc ggtgaagccg gaaatcggct tgccggccgc atcttgaatc tcgacccgga
      721 tgctgccggc agcggaggtg gcgaagttca gttccagctc ctggccgtta aacgtcagcg
      781 gcctggtgag catctcgccc ccgtcgtagc cggcgtgcag cgccgcaaag ccgtcgtgcc
      841 gcagcgaata gcggtggata tgcgccgtcg gctggccgta gttctgattg acgtagagcg
      901 acatttcctc gggtccggtc tgcacgacgt tgagcgcggg atagttggtt cgcgaggtcc
      961 agttctccag gccgagtccc ggcttaagga aaccctccat gaacgtgcgg tcgtagaaat
     1021 caccgccgcg ggtcgtcatc aggatgccgt cggagcaatc gttgaaatag ccgggatcga
     1081 cgcccacccg cgccgcctgc tcgggggtca gcacgcgctt gccgggaaag aagcgggccg
     1141 cgatcgccac gtaaatgtgc ggtgcgcgga agtacgggct cgtttgattc gtatagagtt
     1201 gctcgatcgg cagctttgcc gacggatcgc tttgcaggct atagcccatg agcgtcggct
     1261 cggaccaggt ggcgaagtcc tgcgacgtga cgcgggcgat ccgccgtacg cgatcgacga
     1321 acacgcggta gtaacaggcg tagcactgct ccgcttccga ccaaagtggc acgttctgtg
     1381 aatcgaacgc cccgccggtg atcacgggct cctcgcggag cttcttccag cggatgccgt
     1441 cgggcgagat ccaggcgacc agcccggtgc ggctgctgcc ggcgagggct ttgtagcgct
     1501 cctctgccgg cacgcctttg cggcggtcga tcagcggaca gaagttgtgc gaaaacggcg
     1561 gctggccggc gaggatgacg ttgttttcct tcgtgccgtc cacctcgaag aggcccaggc
     1621 tcggcttcgt ccaggtcttg ccgtcggccg attcggcgta gcacgtcgat tcgcgactgg
     1681 acccatccgc ccgcgattcg ggcaagccgc ggtagtaggc gcggtacttg tcgccgtcgg
     1741 ggatgatcgt cgtgtaagcg ctgaacttgc cttcccacgg cttatccagc gcgaaggcca
     1801 cgccctcgtc gcgcggcgtc tgcagccgca gctccgcgcc gtcgagcttc tcgatcaggt
     1861 agccatcgac aaacaactcc cgccgcgagc cgagttccac tggatcggcg gcgaggacat
     1921 ccatgcacgc tgccgcgatc aacagccaag ccatgacacg aagcatgttc ataatctttc
     1981 cgcgcaattc gtttcattga ctctgttcga cgattcgcca ggcaccattt acatgctcag
     2041 ccggaacctg cttcccattg atgttgacgt atcccttgtg tccgcccgta ggaatgagct
     2101 cgtaccaccg gctgtccacg ctcatggcgt gcttcccgcc gggcccaaag cgcaagtcaa
     2161 aaccaccgtg caagtcgtga tcgacaaagg tgatcgagtc ggacgccatc acatcgggcg
     2221 aatactcgta cgacgtgaac ttgccgtcgg caatggcaaa cgaaaaagtc cggcggtttg
     2281 aatccgtgac gagaagcgaa tcggcacggc catccgcgtc cgtatcttcg tatgcgacac
     2341 ttggattgcg atccggctgc gatggataga cttgggccga cgccctctga ggatcgtcag
     2401 cggcgaggat cgtgaagaga cccacacggc gtcctctggc gctcttcatc cattcgggag
     2461 cgtcctcgcg tagctcctcc ggccagtttt gggtgtaatc ccgcggagac aaagccctgt
     2521 agttggtaac agaccaaatc acccacgcag tgccgaatcc aagcacgaac cagaaaatcc
     2581 gcgacttcat accaaaccgc tttggatccg aagttggtgc cggatccttg tagggattgg
     2641 tatccatggt ttcgcccctt cccgctcacc gtcagcgcca tgtagtacga attccatgcc
     2701 aagaattgaa ttcgattcgc tttactgcgg ctccagttac tgtggcgggt ccacgttcag
     2761 cgggacgaca gccgcccacg agccatcagg caacatccaa ttccgctccc agcgtgttca
     2821 cgtctctgcc ggtggcttgg gcgtacagga catcggggca gaaatcgacg ccagtgggcc
     2881 aggcgagcgt gccagcttcc gaatccaccc tgacctgggc aaaacattca acattttcca
     2941 gcgcctgaaa gaccccgccg cggccaacaa ttcgactacg aaaatccaac tccgcaaccg
     3001 tgccatccga gaaagtgacc tccagaagat agtcgcgcac atggcgaacg taagttactc
     3061 gtggaagcat tggcttgttc ctggtcctag tccagcggtt caattctatg cggcggctgc
     3121 tcattgttca aaagagccca gttgtcgagc aattcctgct ggtgcagggc cgcccactcc
     3181 atgaccaggc ccagtgcacg acgcggcaag gaaccgcgca agaccgttag cggtcgaatg
     3241 ccaacctcga cctctgaacc cgaataaatt gcgtgaaagt gtggaggatt atgagattca
     3301 tccgactgaa gcgtgcgccg ccgcaagtcg ttgcggcagg ctgacttcgc aaacaggatt
     3361 ttttaaggca tggctccggt gtccaatggg tttatgaaag cggcccctgg aagcaatcgc
     3421 caccaggggc c
//
LOCUS       DASUGB010001010         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1780447, whole genome shotgun sequence.
ACCESSION   DASUGB010001010 DASUGB010000000
VERSION     DASUGB010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780447"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            707..1960
                     /locus_tag="VFV87_19095"
     CDS             707..1960
                     /locus_tag="VFV87_19095"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5105937.1"
                     /translation="MNSIQNFRREHQRLARRHFLQLGVAGSAALALLPGMGRADSPPE
                     LAEAIARLESFFTKPEEFRDVSRGKPLPHSLSEEKRTEVGLTRETWKLEVMADAENPT
                     KLARPLTKADNTALDFAGLLALGEKHAVRFAKVMTCLNIGCPLGMGIWEGVPLREVLW
                     LTQPREDLRRVFYYGYHNDDEAQMFKSSLPAGRVLEDPFDLPPVILCYKLNGQWLNPQ
                     RGGPVRVVVPEHYGFKSIKWLSHIVLTNLGYANDTYIGGNNDVDSPLKNFSATLSFPA
                     EAKAGQPLPITGYAQVGISGLSKVQVWISSADKEWPAEDKYFDTAPWTDATILDPPKS
                     WGGNLEGDAVPAGTLGFNDEGQPKTWPMRLGKVHWAAILPGLPAGDYVLRSRTIDERG
                     IAQPLPRPFRKSGHAGIESVNIKIR"
     gene            complement(1982..2317)
                     /locus_tag="VFV87_19100"
     CDS             complement(1982..2317)
                     /locus_tag="VFV87_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105938.1"
                     /translation="MSHAPNPFSNQPNPYASPQMPGSQFQPPLGAEQFAPCPSCRCPY
                     AKRVGFTWWGGILGPKMLTHVKCVHCGQAYNGKTGKSNDTAIAIYLVVSLVIGAAIGL
                     ALGLLSFMK"
BASE COUNT          505 a          710 c          837 g          453 t
ORIGIN      
        1 ctgtgatccg tgcccgcggg tcgcccgcgc cgaaccacgg cctcgcgcca cgaaacgaca
       61 ctggccccgc tcggcgctgg ccgcaaagtg gttcggccaa gcccttggcc gaacggaacc
      121 gccgcgtgcc ccagcagagg ccgaaacggt ggcaagaacc gtaagacgta gtgtcctaac
      181 cgaatcgatt ttcgggcttc gagaaatctg cgaagttctt gtcggacgag ggaaataatc
      241 gggggcgttg gcgcatctat acactggatg cgtgatcgag agattcgcca cggctgattg
      301 ggcgggatgg gcgggcaggg tgtgagtcgt gaagtcagaa gttcgccagg ggggtgccgg
      361 aggacttatt gaagtcctgt gacccccggt ccgtggccgt ggagaaacgg agaactgaat
      421 acgtgaatta aatgcataaa gcgtgaggcg ggaaatgcga cgcaagttgt tcctgcgtca
      481 gcggttacgg cgattcttta tcggcaggaa aaatgttacg gcatgcatga atagagcggg
      541 ggctcggtga ttccggcggg aagcggtgct tgtcgcaaac atgcataaag ctgacggaat
      601 atgcatattc ggggtagggg acgctggcag atgaatcgac gataatgggg gccggccgcg
      661 atcggcgaga cgatgtgatc cgccatcccg atatcaggtg agcgacatga atagcatcca
      721 gaattttcgt cgcgagcatc aacgccttgc ccggcgacac ttcctgcagc tcggtgtggc
      781 tggctccgcg gcgctggctt tgctgcctgg gatgggccgg gccgattcgc ctcccgaatt
      841 ggcggaggcg attgccaggc tggagtcgtt tttcacgaag ccggaggagt tccgcgatgt
      901 gtcgcgcggc aagccgctgc cgcattcgct ctccgaggag aagcggacgg aggtgggcct
      961 gacgcgggag acttggaagc tggaggtgat ggccgatgcg gagaatccga cgaagctcgc
     1021 caggccgctg acgaaggccg acaatacggc gctcgatttc gcggggctct tggcgctggg
     1081 cgagaaacac gccgtgcggt tcgccaaagt gatgacctgc ctcaacatcg gctgcccgct
     1141 ggggatgggc atttgggaag gcgtgccgct gcgcgaggtg ctgtggctga cgcagccgcg
     1201 ggaggatttg cggcgggtct tctactacgg ctaccacaac gacgacgaag cccagatgtt
     1261 caagagttcg ttgccggcgg gccgcgtgct ggaggacccg ttcgacctgc cgccggtgat
     1321 tctgtgctac aagctcaacg gccagtggct caatccgcag cgcggcgggc cggtccgcgt
     1381 cgtcgtgccc gagcattacg gcttcaagtc gatcaagtgg ctctcgcaca tcgtgctcac
     1441 gaacctgggc tatgccaacg acacctacat cggcggcaac aacgacgtcg atagcccgct
     1501 aaagaatttt tccgcaacgc tctcgttccc cgccgaagcc aaagccggcc agccgctgcc
     1561 aatcaccggc tatgcgcagg tggggattag cggcctgtcg aaagtgcagg tctggatttc
     1621 gtcggccgac aaggagtggc cggcggagga caagtatttt gacaccgcgc catggactga
     1681 cgcgacgatt cttgatccgc ccaagtcgtg gggcggcaat ctggaaggcg acgcagtccc
     1741 agcgggcaca cttggcttca atgacgaggg ccagcctaag acgtggccga tgcgcctggg
     1801 caaggttcac tgggccgcga tcttgccggg cctacccgcc ggcgactacg tcctccgcag
     1861 ccggaccatt gacgagagag gcatcgccca acccctgccc cgtccatttc gaaagtccgg
     1921 ccacgcggga atcgagtcgg tgaatatcaa gattcgatag cgtggtccaa cgacaggcga
     1981 ctcatttcat gaacgagagc aatcccagcg cgaggccaat tgccgcgccg atgaccagcg
     2041 agaccacgag atagatcgcg atggcggtgt cgttcgactt gccggtcttg ccgttgtagg
     2101 cctggccgca gtggacgcac ttgacgtgcg tgagcatttt ggggcccaga atgccgcccc
     2161 accacgtaaa gccgacccgc ttggcgtacg ggcagcggca ggaggggcag ggcgcgaact
     2221 gctcggcccc tagcggcggc tgaaactggc tgccgggcat ttgcggcgaa gcgtaggggt
     2281 tgggctggtt tgagaaggga ttgggagcgt gggacatgta tcgtcgtcca accgttggct
     2341 gtcacgcctc gggcttaaag acatccggca gcggcttcga acgtccggga tacattgcga
     2401 taaccatgca agtcaagtag atcgacgcca atgttgccag gatcatcaag tcctgtcggc
     2461 cagcaaacat cacccgccat ccgatttcat gcgcaattac gagca
//
LOCUS       DASUGB010001011         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1917945, whole genome shotgun sequence.
ACCESSION   DASUGB010001011 DASUGB010000000
VERSION     DASUGB010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917945"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFV87_19105"
     CDS             complement(<1..781)
                     /locus_tag="VFV87_19105"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX5105939.1"
                     /translation="MLSVEQALQLVLDHAQPLAPRKMLAAEALGCVLGEDVISDVDSP
                     PHNKSIVDGYAVQAASCPAPGAVLQVVEEITAGIVPSRSVEPATTSRIMTGAPIPEGA
                     DAVVMVEQTAPAGEGRVQIDAPTVKPGQNIMRRASSLARGQTVLQAGIRLRGIELGIL
                     AEVGYQRVAAIPQSKALVIATGNELVDFWQKPAAGQIRNSNSLLLWGLATQAGAFAGQ
                     HGIARDEAAELRTKIATGLNNHVLLLSGGVSAGVLDLVPQVL"
     gene            complement(829..1416)
                     /locus_tag="VFV87_19110"
     CDS             complement(829..1416)
                     /locus_tag="VFV87_19110"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5105940.1"
                     /translation="MILLIDNYDSFTYNLVQRLGEIDPSLEIEVHRNDQITCDQIAAK
                     QPTHIIVSPGPCTPSEAGISVESIQRFAGQVPILGVCLGHQSIGQATGGVIVRAKRLM
                     HGKTDRISHDGQGLFTGLPNPLTATRYHSLVIRPDTLNPDFIVSAWADAPDGAKEIMA
                     IRHKSQPLVGVQFHPESFLTEHGHDMLRAFLKMNV"
     gene            complement(1437..2015)
                     /locus_tag="VFV87_19115"
     CDS             complement(1437..2015)
                     /locus_tag="VFV87_19115"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5105941.1"
                     /translation="MSALPLILASSSPRRRLLLTEAGYDFTVMPPHESAECGVCSRET
                     PPELVARLAFQKAEDVARRTERGIVIGCDTVAECCGQILGKPSNREHARQMLDLLRGR
                     DHHVYSGLCLWRRPDDTHAVEVEVTRLVMDPISDEQLAAYLATGDWEGKAGAFGYQDG
                     LDWVHIVEGSESNVVGLPMELLAQMLPAFRGE"
     gene            2165..2896
                     /locus_tag="VFV87_19120"
     CDS             2165..2896
                     /locus_tag="VFV87_19120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5105942.1"
                     /translation="MPRPTKPPKPPQLNVLYEDNHLLVINKPAGVPTQGVIEGAASIV
                     TLAKAYLKRKYKKPGNVYLGVVSRLDASTSGVLVLARTSKAAARLSAQFRERDADKTY
                     WALAPQPPDPPAGELSHWVLKNDRQARMEIVPPRTRGAQHAKLAYQTLRSENNRTLIE
                     VRLHTGRKHQIRLQLAAIGCPILGDRKYGSRIEFPGPGIALHAARLVIEHPTTKERLC
                     FRALPPTTWGLTGDWLTSLEPPNSA"
BASE COUNT          571 a         1030 c          980 g          544 t
ORIGIN      
        1 ccagcacctg cggcaccaga tcgagaactc cagccgacac gccgcccgac aaaagcaaaa
       61 cgtggttgtt caatccagtc gcaattttcg tccgcagttc tgcagcctca tcgcgcgcga
      121 taccatgctg gccggcaaag gctccggctt gcgtggccag accccacaaa agcagcgagt
      181 tgctgttgcg aatttgcccc gcagcaggct tctgccagaa atcgaccaac tcattgcccg
      241 ttgcaatcac cagtgccttg gactgcggaa tggcggcaac gcgttgatac cccacttcgg
      301 ccaggattcc cagctcgatt cccctaagac gtattcccgc ctgcaacacc gtttgcccgc
      361 gggccaggga tgacgcacgc cgcatgatgt tctggcccgg cttgacggtc ggcgcgtcga
      421 tctgcacgcg gccttcaccg gcgggcgcag tttgctcgac catcacgacc gcgtccgcgc
      481 cctcggggat cggagcgccg gtcataatcc gggaggtggt tgccggttca accgacctgc
      541 tcggcactat gcccgccgtg atttcttcca cgacttgcag aaccgctccg ggagcggggc
      601 aggacgcggc ctgcaccgcg tagccatcga cgatcgactt gttgtggggc ggcgagtcca
      661 cgtcgctgat gacatcttca cccagcacgc aacccagcgc ctcagccgcc agcatctttc
      721 gcggtgccag cggctgcgcg tggtcgagga cgagttgcag ggcttgttcg acggagagca
      781 tgtccgaatt gagggtcaga ggtcgtccct ctgatctctg acctctcgtt acacgttcat
      841 cttgagaaac gcccgcagca tgtcgtggcc gtgctcggtc aggaagctct cggggtggaa
      901 ctgcacgccc acgagcggct gcgacttatg gcggatggcc atgatctctt tcgcaccgtc
      961 cggcgcgtcc gcccaggcgg aaacgatgaa gtcgggattc agcgtgtcgg gccggatcac
     1021 gaggctgtga tagcgcgtgg ccgtcagtgg attgggaagg ccggtgaaaa gtccctgacc
     1081 gtcatgcgag atgcggtccg tcttgccgtg catcaggcgc ttggcccgca cgatcacgcc
     1141 gccggtcgcc tggccgatcg actgatgccc caggcacact ccgagaatcg gaacctgccc
     1201 cgcgaagcgc tggatgcttt cgaccgagat gcccgcttcg ctgggtgtac acggcccggg
     1261 cgatacgatg atgtgcgtgg gttgcttcgc ggcgatttgg tcgcaggtga tttgatcgtt
     1321 gcgatggact tcgatctcca acgacggatc gatctcgccc agccgctgga cgaggttgta
     1381 ggtgaacgag tcgtagttgt cgatcaagag gatcatcgcg tcatcataca atcgccctat
     1441 tcccctcgaa atgccggcag catctgagcg agcagctcca tcggcaagcc gacgacgttc
     1501 gattcgctcc cttccacgat gtgcacccag tccaggccgt cctggtagcc gaaggcgccg
     1561 gctttgcctt cccaatcgcc ggtggcgaga tacgcggcga gttgctcgtc ggagatcggg
     1621 tccataacca gccgcgtgac ctcgacttcg acagcgtgtg tgtcgtccgg ccgccgccag
     1681 aggcacaggc cgctatagac atgatgatcg cgcccgcgga gcaggtcgag catctgccgg
     1741 gcgtgttcgc gattactcgg tttgccgagg atttggccgc agcattcagc gaccgtgtcg
     1801 cagccgatga cgattccgcg ctcggtccgc cgggccacat cctccgcttt ctggaacgcc
     1861 agccgggcca ccaattccgg cggcgtttcg cgactgcaga cgccgcactc ggccgattcg
     1921 tgcggaggca tgaccgtaaa gtcgtagccg gcttcggtca ggagcaagcg gcggcgcggc
     1981 gagctgctgg cgaggatgag gggcaaggcg gacatgctcc catcgtagcc aaagcagccg
     2041 catcgtttaa cggcgagccg caggcgatgc cgactccacg ctggatcgtt tgcgtcgcct
     2101 tcggctccgc gttaaacgaa ccgcccacta ctccctcgct gacgcttcgg gttagtgtgg
     2161 aggcatgccc aggccgacca agccgccaaa accgccgcag ttgaacgtgc tctacgagga
     2221 caatcacctc ctggtgatca acaagcccgc cggcgtgccg acgcagggag tcatcgaggg
     2281 ggccgcgagc atcgtgacgc tggccaaggc gtatctcaaa cggaaataca agaagcccgg
     2341 caacgtgtat ctcggcgtcg tcagccggct ggatgcctcc accagcggcg tcctcgtgct
     2401 ggctcgcacg agcaaagccg cggcccggct gtccgcccag ttccgcgagc gcgacgcgga
     2461 caagacgtac tgggccctcg ccccgcagcc gcccgatccg cccgcgggcg agttgtcgca
     2521 ctgggtgctg aaaaacgacc gccaggcgcg gatggaaatc gttccgccgc gcacccgcgg
     2581 cgcgcagcac gcgaagctcg cctaccaaac gctccgcagt gagaacaatc gcacgctcat
     2641 cgaagtccgc ctccacaccg gccgcaagca ccaaatccgc ctgcagcttg ccgcaatcgg
     2701 ctgcccgatc ctcggcgacc gcaaatacgg cagccgcatc gaattccccg gccccggcat
     2761 cgccctgcac gcggcacgcc tcgtgatcga gcatcccacg accaaggagc ggctgtgctt
     2821 tcgcgcgctg ccgcccacga cgtggggatt gacaggcgat tggctaacgt cgttagagcc
     2881 accaaactcg gcttgacttg cgaaggggaa tgggaacaat tccccgcgtt ttgccgggca
     2941 ctcgcgcggc aaagcgtgct agcggccagg agggcgctag cagcagcagg atcgacagga
     3001 ggttgaatct gcgagcgtgg caggctggaa agcctgcccc atgaagagtg gcgggctgga
     3061 agcctgcccc acgaaagccc aggcaaggag gccggcgatg gtgcgttctg cagtttcgcg
     3121 ctcgg
//
LOCUS       DASUGB010001012        15866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_111372, whole genome shotgun sequence.
ACCESSION   DASUGB010001012 DASUGB010000000
VERSION     DASUGB010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 15866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15866
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111372"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1425
                     /locus_tag="VFV87_19125"
     CDS             112..1425
                     /locus_tag="VFV87_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5105943.1"
                     /translation="MTNAAQPPARPCGTVGIIGLGYVGLPLARAFAGVGVRAIGFDID
                     AAKVEKLNAGKSYIQQIPDSAIAKMREQRFEATADFRRLCEPDAVLICVPTPLTESRE
                     PDLTYVIKSAEAVAAALRPGQLVVLESTTYPTTTRNVVLPILEKSGLKAGQDFFLAFS
                     PEREDPGNQTHALNTIPKVVGGLDPASSERACALYGAIVPKVVQVSSPEVAEACKILE
                     NTYRAINIALVNELKVLYDKMGIDVWEVIDAAKTKPFGFQAFYPGPGLGGHCIPIDPF
                     YLTWVARQYGFATRFIELAGEVNTSMPAYVVSRLGDALNERGKPVKGSKVAILGMAYK
                     KDVDDPRESPGFELMDLLLKKGATVTYNDPHIPALPPMRHYPHLRMQSQKLTAEYLAA
                     QDCVLIATGHSAYDYAWIVEQSPLVVDTRNATRDVTAGREKIVKA"
     gene            1428..2027
                     /locus_tag="VFV87_19130"
     CDS             1428..2027
                     /locus_tag="VFV87_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX5105944.1"
                     /translation="MASLPSLIASDLRAKAEWCYESSGWKAIAKTLLTDGTAAMIWYR
                     LMQWARKWRLTPLEMVFNKLNAICCNCIIGRGAEFGPGFVLIHSTGVVINGQVQGGSR
                     VHLEHQVTIGAERRQSPVLGNRVFIGAGAKIIGPVHIGDDARIGANAVVLKDVPAGAT
                     AVGIPAKVVRQRGPVDELGEPSPETPECDEIEEQPASFR"
     gene            2024..3190
                     /locus_tag="VFV87_19135"
     CDS             2024..3190
                     /locus_tag="VFV87_19135"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5105945.1"
                     /translation="MIAAAQYSLWAALGLIAFAYVGYPTAIFALSRLFGRRPVALPLN
                     HDRVPRVSVLVSALNEEEVIAERIENNLAQDYPAERLEIVIASDGSTDRTAEIVGEFE
                     AQYPGRVKLVDYSVRRGKATAVNETLPQVAGDIVVLSDANTMFAKDAISKLARWFADD
                     SIGVVCGKLKLVDSASGKNVDGLYWRYETFLKECEGRLGALLGSNGAIYALRRQDFVP
                     IPPDTIIDDFMIPLLVKIRRGKRIVYDTEAIAHEETAPDVRAEFRRRARIGAGGFQSL
                     FRLSRLALPIYGWTSFAFLSHKVLRWLCPFLLIAALIANLFLLTQPVYQVVFALQVAF
                     YLVAVAGVFLPGNYAAARLVRLTTMFTSMNLALAVGFWRWVAGKQRGTWQRTAR"
     gene            3271..4380
                     /locus_tag="VFV87_19140"
     CDS             3271..4380
                     /locus_tag="VFV87_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105946.1"
                     /translation="MSERLHLFICVADHYEPQWNSPPASVAAERVQRWIDGYPRMAEG
                     LEDSTGRPPQHTFFYPEEEYEPGYLEALAGLCRAGYGDVEVHLHHDHDTADGLREKLD
                     RFTSVLHHEHGLLERNDAGQITYGFIHGNWALDNSRPDGRWCGVNNELSVLMETGCYA
                     DFTMPSAPAECQTSTINSIYYAADDPERPKSHDQGSRARVGSAPRDKTLLMIQGPLAL
                     DWSSRKWGLLPRIENGDLTLPRPPTLERLQLWRRAHVHVTGRPDWLFVKLHTHGAQER
                     NAQMLLGEPMRRFHQQLRRFAGENEWFRYYYVTAREMASLVHQAESGATRPCIARTAN
                     PVANPIYPSDRDGGESASVSSGAGLELRTESSQSK"
     gene            complement(4590..4964)
                     /locus_tag="VFV87_19145"
     CDS             complement(4590..4964)
                     /locus_tag="VFV87_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105947.1"
                     /translation="MSHWKTVLVTGACVFLALACSSSVSAQVPRYAPPGGSPLPAALD
                     YFRQDVGLLDPYNTFVAPRRQLTNQLQAITARQQADFRAAEAQISALRGASAAPTGVG
                     AGYMNYSHYYSISPVGRSRPGR"
     gene            5202..6137
                     /locus_tag="VFV87_19150"
     CDS             5202..6137
                     /locus_tag="VFV87_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5105948.1"
                     /translation="MTKCLVTGGCGFIGSHLVEALVRRGDTVRVLDDLSTGHAANIEN
                     VRLEVEFIRGTILDAEAVGKAMAGVELVFHQAALASVPRSVADPLATHHACVTGTVNV
                     LDQARRAGVRRVMYAASSSAYGNSHWASKREADPPAPLSPYAAAKLAGEQYCQAFWQS
                     YGLETICLRYFNVFGPRQDPHGPYAAVIPAFILKMQSGQRPMIFGDGKQSRDFTYIAN
                     VVAGNLAAACAAGVAGKVFNLASGRSYSLLDLVAALNHILNLNIGPVFEPARVGDVKD
                     SLADISLSQAELGYAPTVDFSDGLQQTAEYYAAAK"
     gene            6222..6539
                     /locus_tag="VFV87_19155"
     CDS             6222..6539
                     /locus_tag="VFV87_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105949.1"
                     /translation="MAKLFFQELDQRRVSISMPTGGDRTIVHGLATYCRESEFGSCLR
                     IAIDDPAGPLEVVLHEGEWINRLIVTGESKDFLIIPDSQPKIAASQKEEAGMPLGQNC
                     SKS"
     gene            complement(6584..9760)
                     /locus_tag="VFV87_19160"
     CDS             complement(6584..9760)
                     /locus_tag="VFV87_19160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5105950.1"
                     /translation="MSRNGQAFGFIPPADADHASKRDDGSETLLGLDRQTALTPEEES
                     SRRPRLGKFQIVRLLGAGAFGVVYVAIDEQLGREVALKLPRTTVLTDPDLKARFLREA
                     EALGRLDHPNIVPVFEAGECDDTCYLAVGYCEGPTLAEWLRERASPTPPQTAAQIALA
                     LANAVAHAHDRQILHRDLKPSNILLDAQATHDGLEFTPRLTDFGLAKIAEQPTQNTLS
                     GMVLGTPNYMAPEQAAGLVERIGPATDVYSLGAVLYELLTCRPPISGKTTIDTLRRVL
                     IDEPVPVRRLNHTVPEDLEAIVAKCLDKSPARRYPAAVELADDLGRFLAGETTRARPL
                     GVVERSTRWIRRHRMKSAMAALVLLIACLAAGLCAYDQRLRDAQARAAEVTAKSSQLE
                     RQVATSARYVADMSYVADIHAADQAVVAGDLSQAAAILRRRTPGPGKPDLRGLAWHYL
                     WARTIRQTESEADVGTEVYFLRISADAHHLAAACKDGTVRFFDPWSLTEKRTLNTGQG
                     EVNGIDFDDEVRHLATAGDDGTVKIWRLNDETPQYTIRVMPSHAYGALFYSGSTSLFA
                     YGVEEHARLWEIAKAKEIAGLGGHARAIEAMAMSPDRRLLATASSDETATLWDAETLQ
                     PLRKVQHFGRVSSVAFSPDSRLVATGSLDRRLLVWDTRPDGWIAEGYHLDPVQCVAFS
                     ADGRHVIAGDRGGAIRNWRVPPPESSSALTLDSLGDAWQAHQGRVWSLAVLPAADKFI
                     SSGEDGRICLWGRGQPTRRTIATQDQESYESAATTRSGSLLFAARSSGVAAYDASTAK
                     KLFDLPGSASNYSCLTLLEDIDCIAAGTRNGQFIVWNWKTRQQTLQRDFGPDRHIFQL
                     AYASKTNRLAVLSGNDCWLLDAKTGEDRGAVFAEACNDVAFSPEGDRLAIDTLDSIVL
                     VDVASCRRLRQFPAHSSTVEGLDWSPDGNLIASASNDRTVRLWRPNGTLVATLSGHRH
                     AVTGVAFTPDGRSLITCDAGGRAQVTHVATQQELLVIPGLAPLTRLVTPFPDSRRIVF
                     IDEAGAILIVGRPSAVATSSADPTANID"
     gene            complement(10052..10690)
                     /locus_tag="VFV87_19165"
     CDS             complement(10052..10690)
                     /locus_tag="VFV87_19165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5105951.1"
                     /translation="MSKEATHSRVPEAPSTRVTLLARLREAGDEQAWETFVDLYLPLV
                     YRYCRSRGLQDADACDVTQQVLVSVHGAISSFDYDPQRGRFRDWLGTIAYRAVLRQQQ
                     KDRRPGKGQGAGESDLLAGQAGGPVDAAWLEEFNVHIFSAAVERIRPEFDESTWQAFD
                     WTWLHDVKTQEAAQRLGTTSAWVYKARFRVLQRLRSEIEFLTNDVAALHRPG"
     gene            11113..11676
                     /locus_tag="VFV87_19170"
     CDS             11113..11676
                     /locus_tag="VFV87_19170"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX5105952.1"
                     /translation="MPTLAPMPCCYPENLLEDFTTGPDFAPERKWWAVYTKPRQEKAL
                     ARDLADKGVPMFLPLVAKQSLVRGRRVRSEFPLFTSYIFAFCDAQERIVTLDTKRVVQ
                     MVAATDQAAMTQDLRNIHSLVAAGAPLTVEARLQPGQRVRVKNGALKGVEGIIVSRRG
                     EDRLVVAVEFLQQGVSIVIGDYQVEPV"
     gene            complement(12067..13128)
                     /locus_tag="VFV87_19175"
     CDS             complement(12067..13128)
                     /locus_tag="VFV87_19175"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX5105953.1"
                     /translation="MRGVLLTAGLATFAVLAGCSAAHPHHLPALDTVVPSPDMPRELS
                     KIVLPTYTIEPPDILVVEAIHIVPRSPYLLRTGDVISVNVLRALPDSPIAGAYVIQPG
                     GAVNFGVPYGAVKVSGLSIEQAEAGIRSFLVNHLKEPFSVSASLVEIASKQQIAGQHL
                     VGMDGNVTLGSYGNVSVVGRTIAEAKQAIEEHLGQFLEDPEVAVDVFALNSKVYYVIT
                     EGAGLGDTVSRFPVTGNETVLDAIANVSGLTEVSSKKIWIARPVPCSEDVHLLPVDWR
                     GITALGQATTNYQVLPGDRIFIAENELVAFDSQLAKILAPVERAMGFTLLGTGTATRL
                     SGKVLRGGGNPNSGGFSGR"
     gene            complement(13783..14832)
                     /locus_tag="VFV87_19180"
     CDS             complement(13783..14832)
                     /locus_tag="VFV87_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105954.1"
                     /translation="MARAAVPLCLALLAACSPGYAQQNVIDDFGYAPQTGLTPASPQP
                     YCLCGEDGLGMPPCRQWWCSSWYVRAEALFLLRTNGFQDQVLVENNAGAGLLSTNDLS
                     FDLEPGVSALLGHRIDNVSAWELSYFGALQWQSKLQAASLANLDLPDPLGAELDDFDN
                     ADLISIAFASTLHNVEVNYLRDFACFSALVGFRYLSWEEQLGLQALDGDGDISNYDVG
                     TSNNLFGAQLGARTVLHAQWVDWECTGRAGIFGNDAQQRQLLTDDDNAISVRNTGSRA
                     RGAAFVGDINLSAHCRLSRVWSLRAGYGGLWITGLALAPDQLDFSNNLTSGTALAQNG
                     SAFVHGLNVGLEALW"
     gene            15123..15299
                     /locus_tag="VFV87_19185"
     CDS             15123..15299
                     /locus_tag="VFV87_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105955.1"
                     /translation="MRADLREDGWWLIFDGGEEAGPFKQLDDAQAFLDLVDNLQRQQK
                     QDNNSLDSDRSTPA"
     gene            15446..>15866
                     /locus_tag="VFV87_19190"
     CDS             15446..>15866
                     /locus_tag="VFV87_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105956.1"
                     /translation="MQGSVTQRVRFFLAALLACWQLSSAGVLRAGDGQAKEIKLVQAQ
                     TPIQPAAGALVSPGPAEAESKSAAVSLPERVARLQRTIEGDRSRVGELTSQIDNPDSE
                     YKQAEAEFLALDEGRTALVKDIQALREAAKAGEAEALI"
BASE COUNT         3205 a         4725 c         4792 g         3144 t
ORIGIN      
        1 gtgcgaaggc gagttctaga gtttcgtttt gacgtttctg accatcgcaa tatcatggaa
       61 acatcctcct tcgagtcagg ttcccgcagc accgccactg agtccagcga aatgacaaac
      121 gctgcacaac cgccagcgcg cccttgcggt acggtcggca tcatcggcct gggttatgtc
      181 ggcttgccgc tggcgcgggc gtttgcgggg gtgggtgtgc gggcgatcgg gtttgacatt
      241 gatgcggcta aggttgaaaa gctgaatgcg ggcaagtcgt atatccagca gattccggac
      301 agcgcgatcg ccaagatgcg cgagcagcgg tttgaggcca cggcggactt ccgccgactc
      361 tgcgagccgg atgcagtttt gatctgcgta ccgacgccgc tgaccgaatc gcgcgagccg
      421 gatctgacgt atgtcatcaa atcggctgag gctgtggcag ccgcgctgcg gccagggcag
      481 ctcgtcgtgc tcgaaagcac gacgtacccg acgacgacgc gaaacgtcgt ccttcccatc
      541 ctggagaaat cgggcctgaa ggcgggccag gactttttcc tggcgttcag ccctgaacgg
      601 gaagatcccg gcaatcagac gcacgccctg aatacgattc ccaaggtcgt cggcggcctc
      661 gacccagcca gcagcgagcg agcctgcgcc ttgtatggcg ccattgtgcc gaaagtggtg
      721 caagtgagtt cgcccgaggt cgccgaggcc tgcaaaatcc tcgaaaacac gtaccgggcg
      781 atcaacatcg cactggtcaa cgaactgaag gtgctctacg acaagatggg catcgacgtg
      841 tgggaagtga tcgacgcggc caagaccaag ccgttcgggt tccaggcgtt ctatccgggg
      901 ccgggcctcg gcgggcactg catcccgatc gatccgttct atctgacttg ggtcgcccgg
      961 cagtacggct ttgcgacgcg gttcatcgag ctggccggcg aggtgaacac gagcatgccg
     1021 gcttacgtcg tcagccggct tggcgatgca ctcaacgagc ggggcaaacc ggtcaaaggg
     1081 agcaaagttg cgattctggg gatggcctac aagaaggacg tcgatgaccc gcgtgaatcg
     1141 cccggatttg agctgatgga tttgctgctc aagaagggag cgaccgtcac gtacaacgac
     1201 ccccacatcc cggctctccc gccaatgcgg cactatccgc acctgcggat gcaaagccag
     1261 aagctgaccg cggagtacct ggccgcccag gactgcgtgc tgatcgccac gggtcattcg
     1321 gcctacgact atgcctggat cgtcgagcag tcgccgctcg ttgtcgatac gcgaaacgcg
     1381 acgcgtgacg tgaccgccgg ccgcgagaag atcgtcaaag cctgaccatg gcatcgctcc
     1441 catccctgat tgcgtccgac ctgcgggcca aagccgagtg gtgctacgaa agcagcggct
     1501 ggaaggccat tgccaagacg ctgctgaccg acggtaccgc tgcgatgatt tggtatcggc
     1561 tgatgcagtg ggcgcgaaag tggcggttga cgccgctcga gatggtcttc aataagctga
     1621 atgctatctg ctgcaactgc atcattggcc gcggcgctga atttggtccg ggatttgtgc
     1681 tgattcactc caccggcgtc gtcattaacg gccaggtcca gggcggaagc cgcgtgcatc
     1741 tcgagcatca ggtgacaatc ggcgccgagc gccgacagtc gcccgtcctt ggcaaccgcg
     1801 tcttcattgg agccggcgcg aagatcatcg ggccggtcca catcggcgac gatgcgcgga
     1861 tcggcgccaa cgccgtcgta ctcaaggatg tacccgcagg cgctaccgcg gtcggcatac
     1921 cggccaaagt ggttcgtcag agaggtccgg tggatgaatt aggagagccc tccccggaaa
     1981 cgcccgagtg cgacgaaatc gaggagcagc cggctagttt tcgatgatcg ccgctgctca
     2041 atacagtttg tgggccgcgc tgggcttaat tgccttcgct tacgttggct accccaccgc
     2101 gatcttcgcg ctctcacgcc tgttcggccg ccgacccgtc gcgctgccgc tgaatcacga
     2161 tcgggtgccg cgggtcagcg tgcttgtctc ggccttgaac gaagaagagg tgattgccga
     2221 acggatcgag aacaatctgg cccaggatta tccggccgag cggctggaga ttgtgatcgc
     2281 ttccgacggg agcaccgatc gcaccgccga gattgtgggg gaatttgaag cacagtaccc
     2341 cggacgcgtc aaactcgtcg attattccgt ccgccgaggt aaagctaccg ctgtcaacga
     2401 gacacttccg caggttgcgg gcgacatcgt cgtcctctcc gacgccaata ccatgttcgc
     2461 caaagacgcc atcagcaagc tggctcgatg gtttgcggac gattccattg gcgtcgtctg
     2521 cggcaagctc aaactcgtcg attctgcctc cggaaagaat gtggacggcc tctattggcg
     2581 gtacgagacg ttcttgaagg aatgcgaagg tcggctgggg gcgctcttgg gctccaacgg
     2641 agcgatttac gccctgcggc ggcaagattt cgtgccgatc ccgcccgata cgatcatcga
     2701 cgacttcatg attccgctct tggtgaaaat tcgccgcggc aagcgaatcg tctatgacac
     2761 cgaggcgatc gcccacgagg agacggcgcc cgacgtgcgg gcggagttcc gccgccgggc
     2821 gcgcatcggc gcgggcggat ttcagagcct gtttcgactc agtcggctcg cactgccgat
     2881 ctacggctgg acgagtttcg ccttcctgtc gcacaaagtg ctgcgctggc tatgtccgtt
     2941 cctgctcatc gccgccctga tcgccaatct tttcttgctg acgcagccgg tgtatcaagt
     3001 ggtgtttgct ctgcaggtgg cgttctacct ggtggcggtg gcgggagtgt tcttgccagg
     3061 gaactatgct gcagcgcgac tagtgcgact gaccacgatg ttcacttcga tgaacctggc
     3121 attggcggtc ggcttttggc gatgggtcgc aggaaaacag cgcgggacat ggcagcggac
     3181 ggcacgataa gcttccatct cagcatgccc aagattcgtg gattatcgcc gacccaagtc
     3241 gcgcaattag catttccccc tagagttccg gtgagcgagc ggctgcacct tttcatctgc
     3301 gttgccgatc attacgagcc gcagtggaat tcgccgccgg cgagcgtcgc tgccgagcgc
     3361 gtgcagcggt ggatcgacgg ctatccgcgg atggcagagg ggctggagga ctccacggga
     3421 cgaccgccgc agcacacgtt tttctatccc gaggaagagt acgaaccagg ttatcttgaa
     3481 gcgctggctg gcctgtgccg cgccggatac ggcgacgtgg aagtgcacct gcaccacgac
     3541 catgatacgg cagatggcct ccgcgaaaag ctggaccggt tcacgagcgt cttacatcat
     3601 gagcacggcc tgctggagcg caacgacgcg ggtcaaatca cctacgggtt tatccacggc
     3661 aattgggctc tcgataacag ccggcccgat ggtcgctggt gcggagtcaa caacgagctc
     3721 tccgtgttga tggagaccgg ctgctacgcc gatttcacga tgccatccgc tcctgccgag
     3781 tgccagacga gcacgatcaa cagcatttac tacgcagccg atgatcccga gcgacccaaa
     3841 tcgcacgacc agggaagtcg ggcgcgagtc ggctcggctc cacgcgacaa gacgctgctg
     3901 atgatccaag gacccctggc actcgattgg agcagccgca aatggggctt gctgccgcgg
     3961 atcgagaatg gcgatttgac cctcccgcga ccgccgacgc tcgagcgact gcaactgtgg
     4021 cggcgggcgc acgtgcatgt cacaggacgg ccagactggc tattcgtcaa gctccacacg
     4081 cacggcgctc aggagcgaaa cgcccagatg ctgctgggcg aaccgatgcg gcggtttcac
     4141 cagcaactcc gccgatttgc tggcgaaaac gaatggtttc gttattacta cgttaccgcc
     4201 cgtgaaatgg cgtcactcgt gcatcaggcg gagagcggag ccaccaggcc ttgtattgcc
     4261 cgcacggcga accctgtcgc caacccaatt tatccgtcag atcgcgatgg cggcgagtcg
     4321 gcgtccgtgt cctcgggagc cggcctcgag ctgcggaccg agtccagcca gtccaaataa
     4381 tcctcgattt ccgtaagcgg cattcccagg cgcagagctg cctcgatggc ctccgtgcga
     4441 tcgagaaacg tcgccgcatg ctcgagcatc aacttcgcga cttcatgaga catgccagca
     4501 cttgcgctgc ggcggtcgtg cgacgttcgc gaaatgacaa aaggtttcac gtccgtaact
     4561 cagttcgcgg acggggcgcc ggcctgctgc taccgtccag gacgagaacg tccgaccggg
     4621 ctaatcgaat agtaatggga gtagttcatg tagccggcgc cgacgcccgt gggagcagcc
     4681 gacgctccgc gcagcgcgga aatctgggct tccgcggcac gaaaatcggc ctgctggcgg
     4741 gcggtaatcg cctggagttg gtttgtcagt tgccgccggg gggcgacaaa cgtgttatag
     4801 ggatccagca agcccacgtc ctggcggaag taatccaagg ctgccggcag tggacttccg
     4861 cccggagggg catagcgagg aacctgtgca gaaaccgagg aggaacaagc caaggcgaga
     4921 aacacgcagg cgcccgtcac gagcaccgtc ttccaatggc tcatacgttc ctctctacga
     4981 cgacgatcgc accgcaaagc ggatgtgaag tgaactttac ctggctcagc acaaccgcct
     5041 attctaacga attgcaggca gttgcgccaa attgtaaagg tttatcggct gcggaacctg
     5101 cgtagccgaa ggcgaatctt ctctgcgggc gcaaaggcaa tgtttgctag cagcactgag
     5161 tcgttgacgc ctaccgcgaa tctgttgggg agtctctgtt catgaccaag tgccttgtta
     5221 ccggcggttg cggttttatc ggctctcacc tcgtggaggc acttgtgcgg cgcggggaca
     5281 cggtccgcgt actggacgat ctttccacgg ggcacgccgc caatatcgag aacgtccgac
     5341 tggaagtcga atttatccgg gggaccattc tcgatgcgga ggccgttggc aaagcgatgg
     5401 cgggagtaga gctggttttt caccaagccg ccttagcctc cgtgcctcgc agcgttgccg
     5461 acccgctcgc gacgcatcat gcgtgcgtca cgggcaccgt caatgtgctc gatcaagccc
     5521 gtcgggccgg cgtgcggcgg gtgatgtacg ccgcctccag cagcgcctat ggaaattcgc
     5581 actgggcttc caaacgcgag gcggacccgc cggcgccact ttctccctat gcggccgcga
     5641 agctggccgg cgagcaatac tgccaggcgt tctggcagtc gtatggactg gagacaatct
     5701 gcctaaggta tttcaacgtc tttgggccgc gccaggatcc gcatggaccc tatgcagccg
     5761 tgattcccgc gttcattctc aaaatgcagt cgggccagcg tccgatgatc ttcggcgacg
     5821 gaaagcaatc gcgcgatttc acgtacattg ccaatgtcgt ggcgggaaat ctggcggccg
     5881 cttgtgcagc aggggtcgca ggaaaagtgt tcaatctggc cagcggccga agttactcgc
     5941 tcctggatct cgtcgccgca ctcaaccaca ttttgaatct gaatatcggg cctgtcttcg
     6001 aaccggcccg cgtcggcgat gtcaaagaca gcctggcgga catctcgctc agccaggcgg
     6061 agctcggcta cgcgcccacg gttgatttct cggatggcct gcaacaaacg gccgagtact
     6121 acgctgcggc caaatgattt gcaattcgga atgtcaactg tcgcctggaa ttagaaacag
     6181 cactcacttg gtctctgtcc gtctaaggcc aatcacttcc aatggctaaa ctgttctttc
     6241 aagagctgga tcagcgtcga gtttcgatat cgatgcccac aggcggtgac cgcaccattg
     6301 ttcacggact ggcgacatat tgccgtgaat ccgagtttgg ctcgtgtttg aggatcgcca
     6361 tcgacgaccc ggccggacct ctggaggtcg tgctccacga gggtgaatgg attaatcgcc
     6421 tgatcgtcac cggtgagtca aaggactttc tgatcattcc cgattcccaa cctaaaatcg
     6481 ctgcttccca gaaggaagaa gctggcatgc cgctagggca gaattgcagc aaatcataag
     6541 aggttttgac tacgggtccg gagagttcca tcgattcgta atcttagtcg atgtttgctg
     6601 ttggatctgc agacgacgtc gcaactgcag acggacgccc cacgataaga atcgcgcctg
     6661 cctcgtcgat gaatacaatc cgccgactgt cggggaatgg agtgactagt ctggtaagcg
     6721 gggccaagcc cggaatcaca agcaattctt gttgcgtggc gacgtgcgtg acctgggcac
     6781 gtccgcccgc gtcacacgtg atgagggaac ggccatctgg agtaaacgcg actcccgtaa
     6841 cggcgtgacg gtgcccgctc aacgtggcga cgagcgtgcc attgggacgc cacagccgga
     6901 ccgtgcgatc gttgctcgcg gatgcaatca ggttaccgtc cgggctccaa tccagccctt
     6961 cgacggtgct ggagtgggcg ggaaattgcc gcaggcggcg gcaactagcc acatcgacaa
     7021 gcacgatgct gtcgagcgtg tcgatcgcta accggtctcc ttcgggagag aaggcaacat
     7081 cgttgcaggc ctctgcaaaa acggcgcctc tgtcttcgcc agttttggca tcgagcaacc
     7141 aacaatcgtt gccgctaagc acggcgaggc gattcgtctt ggaggcgtag gcaagttgga
     7201 aaatgtgccg atctggtccg aaatcccgct gcagggtctg ctgccgcgtt ttccaattcc
     7261 acacgatgaa ctgcccgttt cgtgtgccgg cagcaatgca atcgatgtct tccagcaacg
     7321 tcaggcacga gtaattcgaa gcggaacctg gcaaatcgaa cagctttttc gccgtgctcg
     7381 cgtcgtaggc cgcgacaccg ctgcttcgag ccgcaaagag cagcgagccg ctgcgcgttg
     7441 tcgcggcgct ttcataggac tcttgatcct gagtggcaat cgttctgcgt gtgggttgtc
     7501 cgcgacccca aaggcagatt cgcccgtctt cgccgctgga aatgaatttg tctgcggccg
     7561 ggagcacggc caacgaccag acgcggccct ggtgcgcttg ccaggcatcg cccagagaat
     7621 caagcgttaa ggcgctggat gactccggcg ggggcacacg ccaattgcgg atggcgcccc
     7681 cgcggtcgcc ggcgatgaca tgtctcccat cagcgctgaa tgcgacacac tgtaccgggt
     7741 caagatgata accttccgcg atccagccgt cgggtcgcgt atcccaaacg agtaggcgac
     7801 gatccaggct gccggtggcg acgagcctgc tgtcgggcga gaaggccacg ctgctgacgc
     7861 gcccgaagtg ttgcactttg cggaggggct gcaaggtttc ggcgtcccac aaagttgccg
     7921 tttcgtcgct cgaagcggtt gccagcagcc ggcggtcggg cgacatggcc atggcttcga
     7981 tcgcccgggc gtgccctcca agacccgcga tctctttcgc cttggcgatt tcccaaagtc
     8041 gagcgtgttc ttcaacccca taggcgaata ggctcgtaga gccgctatag aacaacgctc
     8101 cataagcatg cgatggcata acccggatcg tgtattgtgg ggtttcatca ttcagtcgcc
     8161 agatcttcac ggttccatcg tcaccagcgg tggcaagatg gcggacctcg tcgtcgaagt
     8221 cgatgccgtt aacctctccc tggcccgtat tcaacgtcct tttttccgtc agcgaccagg
     8281 gatcaaaaaa ccgaacggtc ccgtccttgc aagcagccgc caggtggtgg gcatcagcag
     8341 atatgcgcaa aaagtacacc tccgttccaa cgtccgcctc agactccgtc tggcgaatcg
     8401 ttcgggccca gaggtagtgc caggccaacc cgcgcagatc gggcttgccg gggccgggag
     8461 tgcgacggcg cagaatagcc gctgcttgcg atagatcccc cgctaccact gcctggtcgg
     8521 cagcatggat atccgccacg tagctcatgt cggccacata gcgagcgctc gttgcaacct
     8581 gcctttccaa ctgcgacgac ttggccgtaa cctctgctgc acgggcctgg gcatcgcgta
     8641 gtctttgatc gtaggcacag agcccggccg cgaggcaagc gattaacaat acaagcgcag
     8701 ccatggccga cttcatgcga tggcgacgaa tccagcgagt cgatcgctcg acgacgccca
     8761 gaggccgggc gcgagtggtt tctccggcga ggaatcgacc caagtcgtcc gccaattcca
     8821 cggccgccgg gtagcgacga gccggcgact tgtcgaggca cttggccact atcgcttcca
     8881 agtcctccgg aaccgtgtgg ttcaatcgcc ggacgggcac gggttcatcg atcagcacgc
     8941 ggcgcagcgt gtcaattgtc gtctttcccg aaatcggcgg gcggcaggtc agcagctcat
     9001 aaagcacggc gccgagggaa tagacgtcgg tggccggtcc aatcctctcg acgagcccgg
     9061 ccgcttgttc gggcgccatg tagttcggtg tgccaagcac catgccgctg agggtgttct
     9121 gtgttggttg ctcggcaatc ttcgccagac caaagtcggt caggcgggga gtgaattcga
     9181 gcccgtcgtg cgttgcttgc gcgtcgagca gaatgttgct cggtttgagg tcgcgatgca
     9241 ggatttgccg gtcgtgtgca tgggcaacag cgttggccag ggcgagcgcg atctgagccg
     9301 cggtttgcgg cggcgtcggc gaggctcgct cgcggagcca ttcggcaagt gttggtcctt
     9361 cgcagtatcc gaccgcgagg tagcacgtgt catcgcattc gcccgcctca aataccggaa
     9421 cgatgttggg atgatccagt cggccaagag cttccgcctc tcgcaaaaat cgggctttga
     9481 ggtccgggtc ggtcagcacc gtcgtgcggg gcagcttcaa ggccacttcc cgcccgagtt
     9541 gctcgtcgat ggctacatac acgacgccga aagcaccggc acccagcagt cgaacgatct
     9601 ggaactttcc caatcgcgga cggcggctgg attcctcttc cggcgttagc gctgtctgtc
     9661 gatcgaggcc cagcagcgtt tccgatccgt catcacgttt tgaagcatgg tcggcgtccg
     9721 cgggtggaat aaatccaaat gcctggccgt ttcgggacac gagccgattc aaggcgatga
     9781 ggcgctcgcg cagctcggat atagacggct cgagtgtgtc gtgcgcagcg cggcctcgcc
     9841 tcagctccaa taggtcgtat acctcctcgt cgttggcgat cgtatcggcg aggcgctcac
     9901 aggcagaaca ttccagcaga tggctggaaa tctgcaactc ttcatcgatt gacagatccc
     9961 cagcgagaag ggcagccaaa gattgcttgg tgaggcaggc cattatcaaa gatggttccg
    10021 gtgcaatccg gcaaaaccga gattcaggaa atcagccagg ccggtgaagt gccgcgacgt
    10081 cattggtcaa gaattcaatt tctgagcgta agcgctggag aactcgaaag cgagccttgt
    10141 acacccaggc cgatgtggta cccagacgct gcgctgcttc ctgagttttt acgtcgtgga
    10201 gccaagtcca gtcaaacgcc tgccacgtcg attcatcgaa ctccggtcgg atgcgctcca
    10261 cggctgccga gaaaatgtga acgttgaatt cctcgagcca agccgcatcg accggcccgc
    10321 cggcttgtcc tgctaacaag tcgctttcgc cggcgccttg ccccttgccg ggacgtctgt
    10381 ctttctgttg ttggcggagt acagcccggt aggcaattgt gcctagccag tcacgaaacc
    10441 gcccgcgttg agggtcgtag tcgaacgaag agatcgcgcc gtgaacgcta accagcactt
    10501 gctgggtcac gtcgcatgcg tcagcatcct gcaggccgcg gctgcggcaa taccgataaa
    10561 ccagcggaag gtagagatcg acaaaagttt cccacgcctg ctcatcaccg gcctcacgaa
    10621 gtctggccag cagcgtcacc cgcgtcgaag gcgcctccgg aacacgggaa tgtgtcgctt
    10681 ctttcgacaa cgaagcctcc ccaagaaact ccctcggatt tttctcgcga tcgcctaagg
    10741 tcccgtgttc caccctttca ggtttgattg acccctaatt tagatcaaac atgtgaattt
    10801 gtcaggaatc ttccacttcg acgggaagaa aacctgacag gccagctatt taattttgga
    10861 ggaaattgcg ggtcaccgca aaactcttcc aaatcttctc ccctgaatct atcgcaacac
    10921 gcactacatt tccagagact tccagactct ggcacccagg agcttcgaat gaatcgaacc
    10981 ggaatctgtt aggcctgcca acctccagcg aaggaggcgt tgcctcctcc gtcttgcctc
    11041 ccccctcctg ccatacgcct ggccaatccg caacaagccg gctgggcgat tacaaagcta
    11101 atcgcaaatc ctatgccaac ccttgcgccc atgccctgtt gttatcccga aaacctcctg
    11161 gaggatttta cgacggggcc cgatttcgcc cccgagcgaa agtggtgggc tgtttatacc
    11221 aaaccgcgac aggaaaaggc gctggcacgt gacctggcag acaaaggggt cccgatgttc
    11281 ttgccgctgg tggcgaagca gagccttgtg cgaggccgcc gtgtccgatc agagtttccg
    11341 ctgtttacga gctatatatt cgccttttgc gacgctcaag agcggattgt gacactcgat
    11401 acgaagcggg ttgtccagat ggtggccgcc accgatcagg cagccatgac tcaagatctg
    11461 cggaacatac attcgcttgt tgccgcagga gcgccgctga ctgttgaagc gcgtttacag
    11521 cctggacagc gagtgcgcgt caagaatggc gctctgaagg gcgttgaagg aataatcgtt
    11581 tcccgtcgcg gcgaggaccg actggtggtc gccgtcgagt tcctgcagca gggcgtgtcg
    11641 atcgtcatcg gcgattatca ggtcgagccc gtctaacttc atcgattcta gtcggcttaa
    11701 ggctccgcga tcggtggttc cgctggcagt tctggctgcg attctggctc ggatattttt
    11761 tcgatcggca gcacctccgc ctgctctggc atttcgtagg agggataggt gacgcattct
    11821 tcgggccagg cacgccaata cgtgccatga aatccgaagc acggcagcgg cgccgaatag
    11881 aaggctggcg cacacggcac tggctggcaa gcgcgatagc tgcaccctcc ggcggcaata
    11941 atcagcccac cggccatggc gcccgcgtgg agccaccatc gcgggttgtg ggactggctg
    12001 agcatgatgg ttgaatcgtc tgaggatcgt gtgacgcgcg ttttgccgtg tgtttgtcag
    12061 gtgcgcttac cggccgctga aaccgccgct attcggattt ccgccgccgc gaagaacctt
    12121 gccggacagt cgagtggcgg tgccggttcc cagcagcgtg aagcccatag cccgttccac
    12181 aggtgcgaga atctttgcca gctgtgagtc gaaggcaacc aactcgtttt cggcgatgaa
    12241 gatgcgatct ccgggcagca cctggtagtt ggtagtagcc tgccccagtg cggtaatccc
    12301 gcgccaatcg acgggcaaaa ggtggacgtc ttcggaacag ggaaccggcc gcgcgatcca
    12361 gatctttttc gacgaaactt ccgtgagtcc gctgacgttc gcgatagcgt ccagaaccgt
    12421 ttcgttgccg gtgaccggga accgactgac ggtgtcgcca agcccagccc cttcggtgat
    12481 gacgtagtac accttgctgt tgagggcgaa aacatcgacc gcgacctcgg gatcctccag
    12541 gaattgacca aggtgttcct caatcgcctg tttcgcctcc gcaatcgtcc gccccacgac
    12601 gcttacgttg ccatagctgc caagcgtaac gttaccgtcc atacccacca gatgctggcc
    12661 ggcgatttgc tgtttgctcg cgatttcgac cagcgaggcc gagacggaaa atggctcctt
    12721 caagtggttg accaaaaacg accggatccc cgcctctgcc tgttcgatgg acagaccact
    12781 gactttcacg gcgccatacg gtacaccgaa attgaccgcg ccaccgggtt gaatgacgta
    12841 cgctcccgcg atgggcgaat caggaagtgc ccgcaataca ttcacggaga ttacatcgcc
    12901 tgtgcgcagc aaataggggc tgcgaggcac aatgtgaatc gcctcgacga ccaggatgtc
    12961 gggcggctca atcgtgtacg tgggcaggac gattttggag agctctcgcg gcatatctgg
    13021 cgaaggaacc actgtgtcga gcgccggcag atggtgcgga tgagcggcac tgcagccggc
    13081 cagcaccgca aacgttgcca agcctgcagt taagagaacg ccgcgcattg aagtgactgg
    13141 gtccaagatt ctcgcggagg cggtgaatcg aagtgaggtc tgcgcggtat gcgcaaagcg
    13201 tttcccttta tcggtcgaat gaaatggggg atttagcaaa actttaacgc gctaattcaa
    13261 ttggctggcg ctggcgcgac cgtcagaccc aggttcgtac ggaaccgcgc cgagtcgaga
    13321 attcgggcat tagcaaggga gcattttttg gatttaatgc tttcgcgaca agcctttctg
    13381 ggggggcgct taaaagctgc aagcgaacac gtgagtacaa ccaccgatga cctacgtttt
    13441 tgatgttgtt ggatcgcgac tccgaaatag tcgccagacg cctggggttt gagccgaatg
    13501 gaaccgcttg cctcgactgc agactcgctc ctgccttcgc actgcgacac ggttgatttg
    13561 tcgcctgccc cgggagaatc cgtcgtcgtt caaggtgggc gattggacgg attgagcgga
    13621 actctcgtct cggcagaaga cggcggacgc tgcaccgtgc aggttttcaa ggatgtgctg
    13681 ttggttgttc cacgagaacg gcttgctcga tcccaacaac cttgaaacct gcggatcccg
    13741 cggcaatgaa gttttcaggc caaaattgcc gtcgaatggc tgttaccaca gcgcttccag
    13801 ccccacgttc aggccgtgga caaacgccga accattctgc gcaagcgccg tgccgctggt
    13861 caggttgttg ctgaaatcga gttggtccgg cgctagtgcc aggcccgtga tccataggcc
    13921 gccgtatcct gcccgcaaac tccaaacccg cgacagccgg cagtgtgcac tcaggttgat
    13981 atcgcccacg aaagccgccc cgcgcgctcg actgccggtg ttgcggacag aaatcgcatt
    14041 atcgtcgtcc gttaacagtt gtcgctgctg cgcgtcgttg ccaaagatcc ccgctcgtcc
    14101 cgtgcattcc caatccaccc attgcgcatg caggaccgtg cgcgctccga gttgcgcacc
    14161 aaacaggttg ttgctggttc caacgtcgta gttgctgatg tcaccgtcgc catcgagcgc
    14221 ctgcaagcca agctgctcct cccaactcag atagcgaaag ccaaccagcg ccgagaagca
    14281 ggcgaaatcg cgcaggtaat tgacctcgac gttgtgcagt gttgatgcaa aagcgatcga
    14341 gatgagatcg gcgttgtcaa aatcgtcgag ttcagcgcca agcggatcgg gcaagtcgag
    14401 attggccagg ctcgccgcct gcaacttcga ttgccattgg agcgcgccga aatacgaaag
    14461 ttcccaggcc gaaacgttgt ctatccggtg acccaaaagt gccgagactc ccggctcgag
    14521 atcgaaggac aagtcattcg tacttaagag tcccgctccc gcattgttct caaccagaac
    14581 ctggtcttga aacccattcg tgcgaagcag aaaaagcgct tcggcgcgaa cgtaccagga
    14641 ggagcaccac cattgcctgc acggaggcat gcccaggccg tcctcgccac acaggcaata
    14701 cggctgcggc gaggcaggcg tcagccctgt ttgcggcgcg tagccgaaat cgtcgattac
    14761 gttctgctgc gcgtatcccg ggctgcatgc cgctagcagg gcgaggcaca ggggaacggc
    14821 ggcacgggcc attggcattg gtctccacgt ttcatccgcg cggccacgaa ctgccgcact
    14881 tcgccattaa attcggcaca accgctacag tcgctgcagt ttatacaggt caacatttac
    14941 gatgcggggg cgtgctggca acaaaccgtt tggacaccaa tcgcttgcca cctcgtaaca
    15001 gaagttgctt tccgttcgga atcgtggcat cttttagatg gcaatcgact cacctgaaac
    15061 caaacacact attgactcga cccgcgtatc tgcggcgccg cgctagctaa ggccgaattg
    15121 caatgcgagc cgacctgcga gaggacggct ggtggctcat cttcgatggg ggcgaagagg
    15181 cggggccttt caaacagctc gatgacgctc aagcgtttct cgatctggtg gacaatctgc
    15241 agcgtcagca gaagcaggat aacaactcgc tcgattccga tcgcagcacg ccggcttgaa
    15301 cgcgggtaag gccgaagcgc aacaaaccga ccgtgttgcg tcgtcgcaat ctcgcacgac
    15361 tgcagacact ggcgcggttg aaccaccagg gctgattggc tatgatccgc ggcccgcccg
    15421 ctttttcccg gcaaggaggc cagggatgca aggttctgtc acgcaacgag ttcggttctt
    15481 tctcgcagcg ctgcttgcgt gctggcagtt gtcctctgcc ggcgtgctgc gggcaggcga
    15541 cgggcaggca aaagagatca agctcgtcca ggcgcaaacg ccgattcaac cggccgccgg
    15601 cgcgcttgtt tctccggggc cagccgaggc cgaaagcaag tcggcggcgg tttcgttgcc
    15661 cgagcgcgtg gcccgcctgc agcggacgat tgagggagat cggtcacgcg tcggcgagct
    15721 aacctcgcaa attgacaatc ccgacagcga atacaaacag gctgaagcgg agtttctcgc
    15781 gcttgacgag gggcgcacgg cgctcgtcaa agacattcag gcgctgcgcg aagcggccaa
    15841 agctggtgag gccgaggcgc tgattc
//
LOCUS       DASUGB010001013         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_530928, whole genome shotgun sequence.
ACCESSION   DASUGB010001013 DASUGB010000000
VERSION     DASUGB010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530928"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFV87_19195"
     CDS             complement(<1..498)
                     /locus_tag="VFV87_19195"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105957.1"
                     /translation="MPASRTSAPAFSRRRLFHTAAAAAAGGALLQDFAGGQDNPAASV
                     ADRGADLKISSLRGFRVGTKAYLKIETNKGLVGWGEITGLDPNVAVALAHSLFELLDG
                     ENPTRIEHLWQKLYRSHRDMRGGPFMTHVIAAIDVALWDITGKAWGVPVYRLLGGPTR
                     DKLRLY"
     gene            complement(698..1729)
                     /locus_tag="VFV87_19200"
     CDS             complement(698..1729)
                     /locus_tag="VFV87_19200"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5105958.1"
                     /translation="MRTRRLGFTLVELLVVIAIIGILVALLLPAVQAAREAGRRMSCQ
                     NNLKQLGVAAHNHHDVLQVLPHAGEYYQESFVPQLHYDPVSHLPLVKEKTRAGWAFQI
                     APFMEQGNIQTGLGAPDLNGDGNTTDDERLAQAVKAVIPSLYCPTRRSPKPNELFSNG
                     GWSRCGFTAITINNVGRGQTDYAGCAGTSGDTTRCAIIRLAGSCSFNRGLIGLEAIND
                     GTANTIMFGEKRLCIRRLYKNPGDDNEGWMTAFDGDTMRYTSLVPQPDHIFNYDDGQG
                     RFGASHPGGFNVVMCDGAVKSISYKIEAVDNGTNVSVVQGTPPTYTGNITLFNRLGMR
                     NDGLPAQVP"
     gene            complement(2143..2580)
                     /locus_tag="VFV87_19205"
     CDS             complement(2143..2580)
                     /locus_tag="VFV87_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105959.1"
                     /translation="MMRGAFIFLGFVTLAVVAGCGSGYTPPAGVIVTGKVVQGGQPVS
                     TAPTEDGYNGAHVNFFTTDGKGGDATVPCDANGNFRIEYAGEGLPPAHYKVSIAVRNG
                     GPETDMLENKIGRDTTDIEFDIPKEKLGGEFDVGTIEINDHLK"
     gene            complement(2726..>3702)
                     /gene="pgk"
                     /locus_tag="VFV87_19210"
     CDS             complement(2726..>3702)
                     /gene="pgk"
                     /locus_tag="VFV87_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5105960.1"
                     /translation="APGSVLVLENVRAYDLETVLWKADEQSLPKLAPQLAALANLLGE
                     NVAKVYVNEALSAGSLDASSTIVPLAMDRVALGQYIAREFEGPMQECLKAELVVFSGI
                     KIDKLDDLEAIIARGSVRTIFSAGSLAMALKKADAQLAGKDFCLGASEDPANKKEAWY
                     IPPPRIEQAKGMLQAGRSRGIEFVLPVDFKLGDGRAVETLQPADQQFDIGPKSIAHFE
                     QEVGQFIISRRGQPAVVFHNGVFGMFEKPEFEAGTKAFIGQLKRMHDAGLKVYLGGGE
                     GGTALQQYAGPNWEKAVTHCFTAGGTVLNALGAEPVPYLVALRSAAPE"
BASE COUNT          734 a         1143 c         1085 g          740 t
ORIGIN      
        1 atacagccgc aatttgtcgc gcgtcggccc gcccagcagc cgatagacgg gtacgcccca
       61 ggctttgccg gtgatgtccc acagggcgac atcgatggct gcgatgacgt gcgtcatgaa
      121 cggcccgccg cgcatgtcgc gatgcgagcg atagagcttc tgccagaggt gctcgatccg
      181 cgtggggttc tcgccatcca atagctcaaa cagcgaatgc gccagggcca cggccacatt
      241 cgggtcgagc ccggtgatct cgccccagcc gacgagcccc ttgttcgtct cgatcttgag
      301 ataggctttc gtgccgacgc gaaatccgcg gagcgagctg atcttcaggt ccgcgccgcg
      361 atcggcgacg ctggcggccg ggttgtcctg tccgccggca aagtcctgca gcaaggcgcc
      421 gccggcagca gcggcggcag cggtatgaaa gaggcggcgg cgcgaaaatg cgggtgcgga
      481 cgttcggctg gcaggcatcg cgtggtggct ccgggcggca gggagggaaa gaagtcgcca
      541 gcggcgggct tagggttccg ccgctggagc ttaaatctgc cgttcgatcc tacgcgatgc
      601 cagcccctac tgcctccctg ccggaaactt cgggaggcaa gacagaaggg accgccaaac
      661 tggtccgcac ccgcgaaaac gggtatgtac gatctcgtta cggaacctgg gccggaagtc
      721 cgtcgttgcg catgcccagc cggttgaaca gcgtgatgtt gccggtgtaa gtgggtggcg
      781 ttccttggac gactgagacg ttggtcccgt tatccacggc ttcgatcttg tacgaaatgc
      841 tctttacggc accgtcgcac atcaccacgt tgaagccgcc cggatgcgag gcgccaaatc
      901 tgccctggcc gtcgtcgtaa ttaaaaatgt ggtcaggctg cggcacgagg gacgtgtagc
      961 gcatggtgtc tccgtcaaac gctgtcatcc agccctcgtt atcgtcaccc ggattcttat
     1021 agagcctgcg aatgcagagc cgcttctcgc cgaacatgat cgtgttcgcc gtgccgtcgt
     1081 tgatggcttc caggccgatc aggccgcgat tgaaactgca actgcccgcc aagcggatga
     1141 tggcgcaccg ggtggtgtca cctgacgtgc cggcacaccc cgcataatcg gtttgcccgc
     1201 gtcccacgtt gttaatcgtg atggcggtaa agccgcagcg gctccacccg ccattggaaa
     1261 agagttcatt cggttttggg ctgcgccgcg tggggcaata cagcgaggga atgaccgctt
     1321 tgaccgcctg cgccaggcgc tcatcgtcgg tagtattccc gtcaccgttc aaatccggcg
     1381 cgccgaggcc ggtttgaatg ttcccttgct ccataaacgg ggcaatttga aaggcccatc
     1441 ccgcacgtgt cttctccttc acgagcggaa ggtgcgagac cgggtcatag tgtaattgag
     1501 gaacgaacga ttcctggtag tactcgcccg cgtgcggaag cacttgcagc acgtcgtgat
     1561 ggttgtgcgc cgccacgccg agctgcttga ggttgttctg gcaactcatg cggcgaccgg
     1621 cctcacgggc ggcctgcacc gcaggcaaca agagagcgac aagaatgcca ataatggcga
     1681 tcaccaccaa caattccacg agcgtaaaac ctagccttcg cgtacgcatc tccgacccct
     1741 cccgcgagaa aaaaggcgta ttccgaccct agcgaccgat cactttttcg cccgatggcc
     1801 gactcggctt cgcgcgctga gacacctcgg aatacgtggt gctcggtgat ccctgaaaaa
     1861 tgccaccacc tggccaagct acggcgtttt gcaaacccct aagcaccgcc gcagattgac
     1921 cccctcaact taacgtcaac tgcctctgac tacaaccgaa aatgcagaat tttggcagcc
     1981 agacacttaa ccccgaaacg tcgccacggt tgcggtggac cgcgaagtta tgaaacgata
     2041 attccatctt caaccatttt tgaccaaatc tgagcgattt ccaactgaaa aatcggacgc
     2101 aactgctttg gtgcaaaggg cttctgtcca gaattcagga aatcacttca ggtgatcatt
     2161 gatttcgatc gtgcccacgt cgaattcgcc tcccagcttt tcctttggaa tgtcaaattc
     2221 gatgtccgtg gtatcccgcc cgatcttgtt ttccagcatg tcggtctcgg ggccgccatt
     2281 tcgcaccgcg attgacacct tgtaatgggc aggaggcaat ccctccccgg catattcgat
     2341 gcggaagttt ccgttggcgt cgcagggcac ggttgcatca ccgcctttac cgtctgtcgt
     2401 aaagaagttc acatgcgctc cgttatagcc atcctctgtc ggtgcagtgc tcacgggctg
     2461 gccgccttgc acgactttgc ccgtgacgat gacgcccgcc ggaggcgtat atccgcttcc
     2521 gcagcccgct acgactgcca gtgtgacgaa ccctaaaaat atgaaagccc cacgcatcat
     2581 ggagagacgc cttgataaac aaaggacgaa tgcccggtaa ctccacacag atgtgcttaa
     2641 tgtttcatgc cgactgcccg tagtcaactg tctggggacc gatgtctgcg agatcccggc
     2701 gaaaaaccag atggaattcc caaggctatt cgggtgccgc actcctcagg gcaaccaaat
     2761 aggggacggg ctcagcgccg agggcgttga ggacggtgcc gccggcggtg aagcagtggg
     2821 tgactgcttt ttcccagttc ggaccggcat attgctgcaa agcggtgccc ccttcgccgc
     2881 cgccgagata gaccttcagc ccggcgtcgt gcattcgctt gagctggcca atgaaggctt
     2941 tggtgccggc ttcgaattcg ggcttctcga acatgccgaa cacgccgtta tggaagacga
     3001 cggccggctg gccgcggcgg gagatgatga attggcccac ttcctgctcg aaatgagcaa
     3061 tcgacttggg gccgatgtcg aactgctgat cggcaggctg cagggtttca acggcccgcc
     3121 cgtcgccaag cttgaaatcg accggcagga caaactcgat tccccgagag cggccggctt
     3181 gcagcatgcc cttcgcctgc tcaatccgcg gcggcgggat gtaccacgct tccttcttgt
     3241 tggccggatc ctccgaggcg ccaaggcaga agtccttgcc ggcgagttgg gcatcggctt
     3301 tcttgagcgc catcgccaag gaaccggcgg aaaagatcgt ccgcacgctg ccgcgggcga
     3361 tgatcgcctc caggtcgtcg agcttgtcga tcttgatccc gctgaagacg accaactcgg
     3421 ctttcaggca ttcctgcatc gggccctcga actcgcgagc gatgtactgg cctagggcca
     3481 cgcgatccat cgccagcgga acgatcgtgc tggaagcgtc caagctgcct gccgacaagg
     3541 cctcgttcac atagactttg gcgacgttct cccccagcaa attcgccagc gccgcgagtt
     3601 ggggcgcgag cttgggcagc gattgctcgt ccgccttcca aagcacggtt tccaggtcgt
     3661 aagcccgcac gttttccagg accaacacgc tcccgggcgc ca
//
LOCUS       DASUGB010001014         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1085178, whole genome shotgun sequence.
ACCESSION   DASUGB010001014 DASUGB010000000
VERSION     DASUGB010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085178"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFV87_19215"
     CDS             complement(<1..902)
                     /locus_tag="VFV87_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX5105961.1"
                     /translation="MPDFEPVLLAQVGRPDSHTRAAYEKAGGYQALRKVLGKIAPKDV
                     IETVKSSGLRGRGGAGFPTGLKWTFLPPNHPGPIYFCLNADESEPGTFNNRILMEEDP
                     HQVIEGLILSCYATNATTAYYYMRCEYPHAWQRTQQAVEECYAAGYLGKNILGSGYSL
                     DVFLHKGAGAYICGEETGLIESLEGKRAWPRIKPPFPAVEGAFRKPTVVNNVETVACV
                     RHILDRGADWFKSIGVPPDPANPRDAGSYGPKLYCLSGHINKPGCYEAPLGITCQELI
                     DRFGGGIWHGKKAKAAIPGGISMGL"
     gene            complement(1011..1493)
                     /locus_tag="VFV87_19220"
     CDS             complement(1011..1493)
                     /locus_tag="VFV87_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5105962.1"
                     /translation="MTTRRVLTDEMIQAIQAYFPRYPSRQAVTLPALHIVNDKLRYVP
                     RQAVVEIAELLGMAPAQVQDTLTFYGYFKQDKPHGRCRLWVCRSISCALRGADDLLDH
                     LSHALHVQPGETTPDGRVTLEYAECLGACEHAPCMRANNDLLKSVTLPQADEYVKGLP
                     "
     gene            complement(1591..2808)
                     /gene="nuoD"
                     /locus_tag="VFV87_19225"
     CDS             complement(1591..2808)
                     /gene="nuoD"
                     /locus_tag="VFV87_19225"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX5105963.1"
                     /translation="MATATSTLDRPQSEQDYLWTLNFGPQHPATHTTLRLVLKLDGER
                     VVDALPDIGYLHSGFEKLGEHLNYNQYVTITDRMNYISPVANGVAWHAAVEKLLGIEI
                     TPRCKYIRTIVTELARISDHLLCTGAMGLDTGAFTFFLYAFYQREVLYDIFETLSGQR
                     FHPSYTRVGGLMFDFTPQVIEKIRRFVRTFPATLEDMERLLNRNRIFVDRTKGVGLLS
                     KEEATNMSATGPIARASGVTRDLRKDEPYLAYGDLDFQVCCATAGDCFARYYVRMAEM
                     RESLRIIHQAVENLPAGPINIGVDERTTLPPKQQVYQTIEGTITHFELVMLNRGFTVP
                     SEEAYAAIEAPNGELGFYVVGDGSDVAYRARCRPPSFIHFALFPQLIRGHTLSDVVAV
                     LGSLNIIAAELDR"
     gene            complement(2833..3333)
                     /locus_tag="VFV87_19230"
     CDS             complement(2833..3333)
                     /locus_tag="VFV87_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5105964.1"
                     /translation="MPTTTETLDALKSQFGELPTSEFRGQTRVVVAKEKLLEAMRWLK
                     EERGFDLLVDVTCVDYLNYRDARDRFGLVYLLASTETNERITVRVFLNEPDLTVPTMY
                     DLWHGADWLEREVFDMYGIRFEGHPDLRRILMPEEYTAFPLRKDYPMQGRGERHNFPF
                     ITRSEA"
     gene            complement(3415..3963)
                     /gene="nuoB"
                     /locus_tag="VFV87_19235"
     CDS             complement(3415..3963)
                     /gene="nuoB"
                     /locus_tag="VFV87_19235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX5105965.1"
                     /translation="MALSQPKTQLPDNVVVTKLDELASWCRKNSLWPMPFATACCGIE
                     LMATGASKHDLARFGAEVFRFSPRQCDLMIVAGRVAMKMLPVLQRIWQQMHEPKWCIS
                     MGACASTGGVFDTYAVVQGIDRFIPVDMYVPGCPPRPEQLIQSIIDLQEKIQREGTIK
                     GTEFELASRQKPKRALVELPLY"
     gene            complement(3954..4367)
                     /locus_tag="VFV87_19240"
     CDS             complement(3954..4367)
                     /locus_tag="VFV87_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5105966.1"
                     /translation="MDSILLPILIFAAIAAAVPIGMLAVPVLLAPRRNSAIKAMPYES
                     GMDPIHDAHRRFDVRFHLVAIAFLIFDVELLFLYPWAVASRDGLGIDAAVADAVQLGN
                     AGYSRGLVFGEALVFIGLLALGLVYAWRKGVFRWR"
BASE COUNT          829 a         1352 c         1507 g          877 t
ORIGIN      
        1 aggcccatgc tgatgccgcc gggaatcgca gctttggctt tcttgccgtg ccagatgccg
       61 ccgccgaagc ggtcgatcag ctcctggcac gtgatgccga gcggcgcctc atagcagccg
      121 ggcttgttga tgtggccgga gaggcaatag agcttcggcc cgtagctgcc ggcatcgcgg
      181 gggttggccg gatcgggtgg gacgccgatc gatttgaacc aatccgcgcc gcgatccagg
      241 atgtggcgga cgcaggcgac cgtttcgacg ttgttgacca ccgtcggctt gcggaaggcg
      301 ccttcgacgg cgggaaacgg cggtttgatg cggggccagg cccgcttgcc ctcgaggctt
      361 tcgatcaggc cggtttcttc gccgcagata tacgcccccg ctcccttgtg caggaacaca
      421 tcgagcgaat agccgctgcc gaggatgttc ttgccgagat accccgcggc gtagcattct
      481 tcgaccgcct gctgcgtgcg ctgccaggcg tggggatatt cgcaccgcat gtagtagtag
      541 gcggtggtgg cgttcgtcgc gtagcaggaa agaatgagcc cttcgatgac ctgatgcggg
      601 tcttcctcca tcaggatgcg gttgttgaac gtgcccggct cgctttcgtc ggcattgagg
      661 caaaagtaga tcggccccgg atggttcggc ggcaggaagg tccacttgag tcccgtcgga
      721 aaaccggccc cgccgcgccc gcggagaccg ctcgacttga ccgtctcgat cacgtccttc
      781 ggcgcgattt ttcccagcac cttgcgcagc gcctgatagc cgccggcctt ttcataggcc
      841 gcgcgagtat ggctgtcggg cctgccgacc tgcgctaaca ggactggttc gaaatcaggc
      901 atcgttcgct tttagccggg agcaaacttg ctcccggaga actgacgatt cgtaatctgc
      961 gcgtgtatcc gcttctccgg gaccaggttt ggtcccagct aaggacaggt tcagggcaat
     1021 cctttcacgt attcatccgc ctgcggcagc gtgacggatt tgagcaggtc attgttggcg
     1081 cgcatgcatg gcgcatgctc gcacgcgccg aggcattcgg catattcaag cgtgacgcgg
     1141 ccatcgggtg tcgtttcgcc gggttggacg tggagggcgt ggctgaggtg gtcgagcagg
     1201 tcgtcggccc cgcggagggc gcagctaatc gagcggcaga cccacagccg gcaccgacca
     1261 tgcggcttgt cctgcttgaa gtagccgtag aacgtgagcg tgtcctgcac ctgcgccggg
     1321 gccatgccga gcaactcggc gatctcgacc accgcctgcc gcggcacgta gcgaagcttg
     1381 tcgttgacga tatgcagcgc cggcagcgtt acggcttgcc gcgacgggta gcgcggaaag
     1441 tacgcctgga tggcctgaat catttcgtcg gtgagcactc gtcgtgtcgt catgcgtagc
     1501 gagtgcggaa tggggaatgc ggaatgcgga atggagacag aatcatctgc tccgaactcc
     1561 gcattccgaa ttccgaattc cgcatttgtt tcaccgatcc agttcagcgg caatgatgtt
     1621 cagacttccc agcaccgcca ccacgtcact gagcgtgtgg ccgcggataa gctgcgggaa
     1681 gagggcgaag tgaatgaatg atggggggcg gcatcgtgcg cgataggcca cgtcgctgcc
     1741 gtcgccgacg acgtagaacc cgagctcgcc attgggcgct tcgatcgcgg cataggcttc
     1801 ttcactgggc accgtgaagc cgcggttcag catgaccagc tcaaaatgcg taatcgtgcc
     1861 ttcgatcgtt tgatagacct gctgcttggg cggcagcgtg gtccgctcat cgacgccgat
     1921 gttgatcggc ccggccggca ggttctcgac ggcctggtgg ataatccgca ggctttcgcg
     1981 catctcggcc attcgcacgt aatagcgggc gaagcaatcg ccggctgtgg cgcagcagac
     2041 ctgaaaatcg aggtcgccgt aggccaggta cggctcgtcc ttgcgcaggt cgcgcgtgac
     2101 gccgctggcg cgggcgatcg ggccggtggc gctcatgttg gtcgcctctt ccttgctgag
     2161 caggccgacc cccttggtcc gatcgacgaa aatccggttg cgattgagca gccgttccat
     2221 gtcctcgagc gtcgcgggaa aagtccgcac gaagcggcgg atcttctcga tcacctgcgg
     2281 cgtgaaatcg aacatcaggc cgccgacgcg agtgtagctg ggatgaaacc gctgcccgct
     2341 cagcgtctcg aagatgtcgt agaggacttc ccgctgatag aaggcgtaga ggaaaaacgt
     2401 gaacgccccg gtatccaggc ccatcgcgcc ggtgcagagc aggtggtcgc tgatgcgggc
     2461 cagctccgtg acgatggtgc ggatgtactt gcagcgcggc gtgatctcga tgccgagcag
     2521 cttctcgacc gccgcgtgcc aggcgacgcc gttggcgacc ggcgaaatgt aattcatccg
     2581 gtcggtgatc gtgacgtact ggttgtaatt gaggtgctcg cctagcttct cgaagccgct
     2641 gtgcaggtag ccgatgtcgg ggagcgcatc gacgacccgc tcgccgtcga gcttcagcac
     2701 caggcgcagc gtggtgtgcg tggccgggtg ctgcgggccg aagttgagcg tccacaggta
     2761 gtcttgctca gactgcggac ggtcgagggt ggacgtagcg gtcgccatgg agtgtcagtt
     2821 cgcgggcatg attcacgctt cgctccgcgt gataaacgga aaattgtgcc gctcgccgcg
     2881 gccttgcatg gggtaatcct tgcggagcgg gaaggcggtg tattcttcgg gcatcaggat
     2941 gcggcggagg tccgggtgtc cttcaaaccg gatgccgtac atgtcgaaca cttcgcgctc
     3001 cagccaatcc gcgccgtgcc acaggtcgta catcgtcggc acggtcagat cgggctcgtt
     3061 gaggaagacg cggaccgtga ttcgctcgtt ggtttcggtg ctcgccagca gatagaccag
     3121 gccgaaccgg tcgcgggcgt cgcgatagtt gaggtaatcg acgcaggtca catcgacgag
     3181 caaatcaaag ccgcgctcct ctttcagcca gcgcatcgcc tccagcagct tttccttcgc
     3241 gacaacgacg cgcgtctgcc cgcggaattc gctcgtgggc agttcgccga actgcgattt
     3301 cagggcgtcg agagtttccg tggtagtcgg catatgtcgt gtacatttcg ccgcgtcgct
     3361 cgcgacgcgc tcaagcgcgc tgcaagcacc gcggcgaaat gtgctgatta agtgttagta
     3421 aagcggcaac tcgaccagtg ctctcttggg tttttgtcgc gaagccagct caaactcggt
     3481 ccctttgatc gtcccttccc gctggatctt ctcctgcagg tcgatgatcg actgaatgag
     3541 ctgctcgggg cgcggcgggc agccgggaac gtacatatcg acgggaatga agcggtcgat
     3601 gccctggacg acggcgtagg tgtcgaacac gccgccggtc gaggcacaag cgcccatgct
     3661 gatgcaccac ttcggctcgt gcatttgctg ccagatgcgc tgcagcacgg ggagcatctt
     3721 catcgccacc cggccggcga cgatcatcag gtcgcactgc cgcgggctga agcgaaagac
     3781 ttccgccccg aagcgggcca ggtcgtgctt gctggccccg gtggccatca attcgatgcc
     3841 gcagcaggcg gtggcgaagg gcatcggcca caggctgttc ttgcgacacc aactggccag
     3901 ctcgtcgagc ttggtgacga cgacgttatc cgggagctgg gttttcggct ggcttaacgc
     3961 catcggaaaa cccccttccg ccaggcataa accagaccca gggccaagag tccgataaag
     4021 acgagcgcct cgccaaaaac caggcctcgg ctgtagccgg cgttcccgag ttgcacggca
     4081 tcggcgacgg cggcatcgat gcccagcccg tcgcgactgg cgacggccca gggatagagg
     4141 aacagcaact caacgtcgaa gatcaggaag gcgatggcta cgaggtgaaa gcggacatcg
     4201 aaccgccggt gggcgtcatg gatcgggtcc atgccgcttt cgtagggcat ggctttgatg
     4261 gcactgttcc gccggggggc cagcagcacg ggcacggcca acatgccgat cgggaccgcg
     4321 gcagcgatcg cggcgaagat cagaatcggc agcaggatgc tatccataag ggactgccac
     4381 ccaataactt gcgacgggcc atggactttg tgaattatat cacaagcccc agccaaaaaa
     4441 gaacccgacc gcctgggtcg agtctgcgca caagatccta tcggtgctgc cgggggtggt
     4501 caatcgggca ggctcatgct ggccgacgta cgattgctga ggggaataaa tggacggcgg
     4561 gctgg
//
LOCUS       DASUGB010001015         4328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_530998, whole genome shotgun sequence.
ACCESSION   DASUGB010001015 DASUGB010000000
VERSION     DASUGB010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530998"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFV87_19245"
     CDS             complement(<1..1227)
                     /locus_tag="VFV87_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5105967.1"
                     /translation="MPHFLRNAFPYFAIGVLATALVWALSFGTLPKADFTFDNGTEIE
                     TLDPALATGQPEHRIINGLFEGLLRLGPEGDEWKKAGPKGSVAMVPQPGAAELPQISE
                     DGRTYTFRLRPEARWSNGDPVTADDFAWSWRRTLHPETGSRYAYQLYYLVGAKNYNLA
                     VVNVRDQVEVELPDRKNPLQTFPRGTIVRGTLRSIHKPPEPTIPAGASDEKKADIEAD
                     WKRGWVYAVELEDKSTRWFAKDVRLAGRSTLTPTLSQVEREQNIESCLYVLPDFEATV
                     GVKAKSLEKLVLTLENRTPYFSDLLAFYPLFAVNPKCVETFGSPDWTKAESIVTNGPF
                     KLEFRRIRDRIRMVKNEQYWDSANIRLNVIDAMATKSETTSLNMYLNGQLDWSTQMPV
                     STIPKLKKGYADQFYSG"
     gene            complement(1315..3303)
                     /gene="mnmG"
                     /locus_tag="VFV87_19250"
     CDS             complement(1315..3303)
                     /gene="mnmG"
                     /locus_tag="VFV87_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5105968.1"
                     /translation="MGTATTGRGFGVQNTIGSMSGDCYNFDVLVIGAGHAGVEAALAA
                     ARMGARVGLLTTNCDTVAQMSCNPAIGGVAKGQIVREIDALGGVMGRAIDATGIQFRL
                     LNRRKGPAMHSPRAQADKKGYQFEVKRLVEEQLGLVLRQEVVEDLLVEESGDGGQGTG
                     GQLQIANCKLQIADLEPTTHHSPLTTHRIVGVRVRGGVEYRANAVVLTTGTFLSAIMH
                     TGEVKTPGGRAGEGTTTGISGALHRLGFRIERFKTGTPCRLNGRTIDYGQCELQPGDD
                     DPEPFSFLTDEAARRRIAENQVPCWITHTNEQVHELIRANLHRAPMYSGQIQSRGPRY
                     CPSIEDKVVRFAEKESHQLFLEPEGRNTHEVYVNGISTSLPRDVQDAMLGLIPGLERA
                     EIMRYGYAVEYDYCPPDQLWPWLETKNVSGLFFAGQINGTTGYEEAAGQGLIAGANAA
                     LKLQGQGPLVIERDAAYLGVLVDDLVTCGVDEPYRMFTSRAEFRLLLRHDNADRRLTQ
                     IGREAGLVDDARWQRLEEKEAEIALAMDLLQNRRVEGTTLDQLLRRPEVEWAQVAAHE
                     PQLAAIGREAIQQVVHDVKYAGYVSRQEQQVARQRRLADKRIPESFNFAGIRHLRIEA
                     REKLTRIRPVTLAQASRISGITPADVALVLAHLEGRGA"
     gene            3399..>4328
                     /locus_tag="VFV87_19255"
     CDS             3399..>4328
                     /locus_tag="VFV87_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5105969.1"
                     /translation="MNLPRPLLCVLCVLLFNLPASSAEQVKKVLYIGIDGTRFDAIEK
                     AETPNLDALMKDGIHSPTCLILGDRYQKNDTISGPGWSSILTGVWADKHGVHDNSFKG
                     DRYQEFPHFFARLKEVRPDAKTASFVTWEPIHSKITSAADESINFEQKEHGVLDYDRY
                     DTQATEAAVKQLTEGNPDALFLYIGQVDVAGHTHGFHPTVPQYVEATERADKLVGRAV
                     AAMKARKTYADENWLVIVTSDHGGKGTGHGGGHKTPEILNSFLILSGLSAQRGTFAEQ
                     VYLVDAPVTALAHLGVQAKDEWKLDGKPVGLKAA"
BASE COUNT          765 a         1466 c         1267 g          830 t
ORIGIN      
        1 gccgctatag aactgatcgg cgtagccctt cttcagcttg ggaatcgtgc tgaccggcat
       61 ctgcgtggac cagtcgagct gcccgttgag atacatgttg aggctggtcg tctcgctttt
      121 ggtcgccatt gcgtcgatca cattcagccg gatgttcgcg gagtcccagt actgctcgtt
      181 cttgaccatg cgaatgcggt cgcgaatgcg gcggaactcc agcttgaacg ggccgttggt
      241 aacgatgctc tccgccttcg tccagtcggg cgagccaaag gtttcgacgc acttcggatt
      301 gacggcaaac agcggataga acgccaacag gtcgctgaag tagggcgtgc ggttttcgag
      361 cgtcaggacc agcttttcca gcgatttggc tttcacgccg acagttgctt caaaatcggg
      421 gaggacatac aagcaggact cgatgttctg ctccctctcc acctgggaga gggtcggggt
      481 gagggtgctc cgtcccgcca ggcgaacatc cttcgcgaac caccgcgtcg atttgtcctc
      541 caactcgacc gcgtacaccc agccgcgttt ccagtcggct tcgatgtccg ccttcttttc
      601 gtcgcttgcg cccgcgggaa tcgtcggctc tggcggcttg tggatgctgc ggagtgtgcc
      661 gcggacgatc gtgccgcggg ggaacgtctg cagcgggttc ttgcggtcgg gcagctcgac
      721 ctcgacctgg tctcttacgt tgaccaccgc caggttgtaa ttcttcgcgc cgaccaggta
      781 gtaaagctgg taggcatagc ggctgccggt ttccggatgc agcgttcgcc gccacgacca
      841 ggcgaagtcg tccgccgtca ccgggtcgcc attcgaccag cgagcctccg gccgcaaccg
      901 gaacgtataa gtgcggccat cctcggaaat ctgcggcaat tcggccgcgc ccggctgcgg
      961 gaccatcgcc acactgccct tggggccggc ctttttccac tcgtcccctt ccggccccag
     1021 cctgagcagc ccctcgaaca gcccgttgat gatgcggtgc tccggctggc cggtggcgag
     1081 agccgggtcg agcgtttcaa tctccgtgcc gttgtcgaac gtgaaatcgg ccttcggcag
     1141 cgtgccgaac gagagggccc agacgagcgc cgtcgcgagg acgccgatgg cgaagtaggg
     1201 aaacgcgtta cggaggaagt gaggcatggc ggttaatttt ggattttgga ttatcgattt
     1261 tggattcgcc tgcatccaat ccaaaatcca aaatcggaaa tccaaaatcg catgttaggc
     1321 tcctcggcct tccaaatgtg ctaagaccag cgccacgtcc gccggcgtga tgccgctgat
     1381 gcggctggcc tgggcgagcg tgacggggcg gatgcgggtc agcttttcgc gggcttcgat
     1441 ccgcaggtgg cggatgccgg cgaaattgaa gctctcgggg atccgcttgt cggccagacg
     1501 ccgctggcgg gcgacctgct gctcctgccg cgacacgtag cccgcgtatt ttacgtcatg
     1561 aacgacctgc tggatcgcct cgcgaccgat cgcggccagt tgcggctcat gggcggcgac
     1621 ttgagcccat tcaacttccg gccggcgcag gagctgatcc agcgtcgtgc cttccacgcg
     1681 gcgattctgc agcaggtcca tcgcgagggc gatttccgcc tctttctctt ccaggcgctg
     1741 ccagcgggca tcatcgacca ggcccgcttc gcggccaatc tgcgtcagcc ggcggtcggc
     1801 gttgtcgtgc ctgagcagca ggcgaaactc cgcccggctg gtaaacatgc ggtagggctc
     1861 atcgacgccg caggtcacga ggtcgtcgac cagcacgccc aaataagccg cgtcccgctc
     1921 gatcaccagc ggtccctggc cctgcagctt gagcgccgca tttgccccgg cgatcagccc
     1981 ttgcccggcg gcttcttcgt aaccggtcgt gccgttgatc tgcccggcga aaaataggcc
     2041 gctgacgttt ttcgtctcca gccacggcca aagctggtcc ggcgggcagt agtcgtattc
     2101 caccgcgtag ccataccgca tgatctccgc ccgctccagc cccggaatca gccccagcat
     2161 cgcgtcctgc acgtcccgcg gcaggctggt ggagatgccg ttgacataga cctcgtgcgt
     2221 gttgcgtcct tcaggctcca ggaagagctg atggctttcc ttctcggcga accgcacgac
     2281 cttgtcttcg atcgacgggc aataccgcgg cccgcgcgac tggatctgcc cgctatacat
     2341 cggcgcccgg tgcaggttcg cccggatcag ctcatgcact tgctcgttgg tatgcgtgat
     2401 ccagcacggc acctggtttt cagcaattcg tcgccgcgcc gcctcatcgg tcaaaaacga
     2461 gaatggctcc gggtcatcat cccccggctg caactcgcac tggccgtagt cgatcgtccg
     2521 cccattcagc cggcaaggcg tccccgtctt gaaccgctcg atccgaaacc ccagccggtg
     2581 caaagccccg ctgatccccg tcgtcgtccc ctcgccagct cggccaccgg gcgtcttcac
     2641 ctcgccggtg tgcatgatgg cggagagaaa agtgcccgtg gtgaggacga cggcattggc
     2701 gcggtactcc acgccgccgc ggacgcgaac gcccacgatc cggtgagtgg tgagtggtga
     2761 gtggtgagtg gtgggctcca aatctgcaat ttgcaatttg caatttgcaa tttgcaattg
     2821 cccccctgtc ccctgtcccc cgtcccctga ctcctcgacc agcaggtcct ccaccacctc
     2881 ctgccttagc accagcccaa gctgctcctc caccagccgc ttgacctcga attgatagcc
     2941 cttcttgtcg gcctgggccc gcgggctgtg catcgccggc cccttgcggc ggttgagcag
     3001 gcggaactga atgccggtgg cgtcgatcgc ccgccccatt acgccgccga gcgcgtcgat
     3061 ctcgcggacg atctgtccct tggccacgcc gccgatcgcc gggttacagc tcatctgggc
     3121 gaccgtatcg cagttcgtcg tcagcaatcc gacgcgggcc cccatccgcg ccgccgccag
     3181 cgccgcctcc acgccggcat gccccgcccc aatcaccagc acatcaaagt tgtagcaatc
     3241 cccgctcatc gaccctatcg tattctgtac cccaaagccg cgaccagtgg tcgcggttcc
     3301 catacaacaa cgggacaatg catacggcgc gcacgttcac aaaccgcccc tggattcaat
     3361 caaccgtact ccgcgaccac tggtcgcggc tttgtgtcat gaacctccct cgcccgctgc
     3421 tttgcgtcct ctgcgttctt ctgttcaatt tgcccgcttc gtccgctgag caagtcaaga
     3481 aggtgctcta catcggcatc gacggcacgc ggttcgacgc gatcgaaaag gcggaaacgc
     3541 cgaatctcga tgcgctcatg aaggacggca tccattcgcc gacttgcctg atcctcggcg
     3601 accgctacca gaaaaacgac acgatcagcg gccccggctg gtcgagcatt ctcaccggcg
     3661 tatgggccga caaacatggt gtccacgaca acagcttcaa gggtgatcgc taccaggaat
     3721 tcccccactt cttcgcccgg ctgaaggaag tccgccccga tgccaagacc gccagcttcg
     3781 tcacctggga gccgatccac agcaagatca cgtccgcggc cgacgagtcg atcaacttcg
     3841 agcagaagga gcacggcgtc ctcgattacg accgctacga cacgcaggcg accgaggcag
     3901 ccgtcaaaca gcttaccgag ggcaatcccg acgctctttt cctctacatc ggtcaggtcg
     3961 atgtggccgg ccacacccac ggctttcatc cgacggtgcc gcaatacgtt gaagcgactg
     4021 agcgggccga taagctggtc ggccgggccg tcgccgccat gaaggcccgc aagacttacg
     4081 ccgatgaaaa ctggctcgtg atcgtcacca gcgaccacgg cggcaaaggg accggccacg
     4141 gcggcgggca caaaactccc gaaatcctca acagcttcct gatcctcagc ggactctccg
     4201 cccaacgcgg cacgttcgcc gagcaagtct atctcgtcga tgcgcccgtg accgccctgg
     4261 cccacctcgg cgtgcaagcc aaggacgaat ggaagctcga cggcaaacca gtcggcctca
     4321 aagccgcg
//
LOCUS       DASUGB010001016         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1500957, whole genome shotgun sequence.
ACCESSION   DASUGB010001016 DASUGB010000000
VERSION     DASUGB010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500957"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFV87_19260"
     CDS             <1..540
                     /locus_tag="VFV87_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5105970.1"
                     /translation="DTFRYWLGDPVRVYASVRPDPRTSKKFKHEDGICLYILEYANGA
                     RASSWDDVWAGPALEGAQKDISIRWRVEGTEGLARGTIGWPDYPSPTPSTLDFTTCKL
                     EGTQKPGYWLSPRWPEVWFPDAFTGTMAQLLVALETLTDPELSGQDNLRTMALVDACY
                     LSAKEHRAVEVKEVLKTKG"
     gene            1290..1658
                     /locus_tag="VFV87_19265"
     CDS             1290..1658
                     /locus_tag="VFV87_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5105971.1"
                     /translation="MKQVQTTLHRGRFQPLAATRRAQVAKMELRPGESSDEEVSNEHP
                     RSEQWLYVVRGTGSATVIPRHGRRRRIPLKAGSLLVIEAGERHQIRNTGRKLLSTVNF
                     YVPPAYSKGGNVLPQTSRSR"
     gene            complement(1709..2956)
                     /locus_tag="VFV87_19270"
     CDS             complement(1709..2956)
                     /locus_tag="VFV87_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105972.1"
                     /translation="MIVTSASPTNSNAAESAAAKSSWAWPPFPVTFIAAGSILVVAVF
                     ANFPAVEATGPTLDGSGRYGPHFELTTEYQHGWPVRYVRRKAWLEVPGYSRERASPWT
                     PWRDPVEFSWSAILIDALAWLGILFCGALLVQMWRSRRRSLWQLRLVDLLGMVTLIGV
                     LIGWGVQQRNERREEHRLWSAARRVPLSISDSLGGVIPAWLPDEYQKRYLDEFGHVLY
                     FTGDAGTACQFPHLLVLQSPGASPEFAELLRRMPQLEALDLFMSDSPQKDEQWRLTSM
                     RDPPPMPNLRGINLYERDITDANLIWLSKCPRLECIELRGNKISDIGLEYLTGLNRLR
                     ILNVSGDAITDRGCQLLAQISSLESLYVDSFGISDAGLAELSRLKRLKALHVEAAISD
                     KAIARLRQELPDCQVSCTIITAR"
     gene            3125..>3880
                     /locus_tag="VFV87_19275"
     CDS             3125..>3880
                     /locus_tag="VFV87_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5105973.1"
                     /translation="MCIVKLFSPFPGWPSKARDWRSLPYRVAALAATIGLGTAGIFLC
                     FVAQGAESAPPNIVVVLADDLGYGDLACFGHPVVKSPNLDRLAREGLRLTSCYSSAAN
                     CSPARCGLMTGRTPYRVGIHNWIPMLSPMHLKAEEVTIATLLKNAGYSTCHSGKWHLN
                     GMFNLPGQPQPGDHGFEHWFSTQNNCLPNHKNPYNFVRSDSVGGPRGGIPVGPLEGYA
                     AQLVAEEAIAWLREGRDKTKPFFLYLCFHEPHEP"
BASE COUNT          756 a         1245 c         1181 g          698 t
ORIGIN      
        1 gacacgttcc ggtactggct gggcgatccc gtgcgggtct atgccagcgt ccgccccgac
       61 ccgcgcacga gcaaaaagtt caagcacgaa gacggcatct gcctctacat cctggaatat
      121 gccaatggcg cccgggccag ctcgtgggac gacgtctggg ccggcccggc cctcgaaggg
      181 gcccagaagg acatctcgat ccgctggcgg gtcgagggga ccgaaggcct ggcccgcggc
      241 accatcggct ggcccgacta tcccagcccc acccccagca cgctcgactt cacgacttgc
      301 aaactagagg gcacccagaa gcccggctac tggctctccc cccgctggcc cgaagtctgg
      361 ttccccgacg ccttcaccgg cacgatggcg cagctcctcg tcgccctcga aaccctcacc
      421 gaccccgaac tcagcggcca agacaaccta agaacgatgg ccctcgtcga tgcgtgctat
      481 ctgtcggcga aggagcaccg ggcggtggag gtgaaagaag tgctgaagac taagggctga
      541 cagtctacca tctattatgg atgattgggc gatgacggac caaatagctc gattccgttg
      601 tggacgagat catccaactg tttgaagtta agggaagttg gctccagttt tgaaaatggc
      661 gaagcgtgac gcttcattcc agagatgaca cctagtgaaa cgcctggttc ggcgacggag
      721 tggcgcttgt cggacgatgc aggcaggggc gttcacgccc ttatgtctct taggcccggc
      781 gtttacgccg ggtcgccggg aggtggcgga ccctatgggt tagccccgtt cacggggctt
      841 ctcgcagcct ggcttcagcc gcctgggcca tcggctgaag ccgcgaaggg gaagccccat
      901 gaatggggct gaccaaatcg ggcgcctgcg ccgctgccgc cctcccgccg tcaacggcgc
      961 gcctgacagg ccaagccccg tgaacggggc tcagcggtgc attctccctg aacggcaggg
     1021 cgggagacct gcggtcggct ggttcggcgg gctggctgtg ggccgctgaa agcgaaacac
     1081 gctggtgggt cctgccggcg aacgatctga tcgacatgac gggacgtggc ttgaggccgt
     1141 ggttcgccgc ggcgatgact cacagccacc ccgtcgctgg ccgcggcact cgctttgctg
     1201 gcaaatccgg aaggtcaaac tctcgcagga gactcgaagc agaatgccat gcagaagtac
     1261 caccttcgcg tgagcgacgg agagcagcca tgaagcaagt ccaaaccaca cttcaccgcg
     1321 gacgatttca gccgctggcg gcgacgcgcc gagcgcaagt ggcgaaaatg gagctgcgcc
     1381 ccggagaaag cagcgatgag gaagtttcga acgagcatcc gcgcagcgaa cagtggctgt
     1441 acgtcgtccg cgggacgggt agcgcgacgg tgatcccgcg ccacggccgg cgacggcgca
     1501 ttcccctcaa ggctggttcg ctgctggtta tcgaggccgg cgagcggcat cagatccgta
     1561 acaccggccg aaagctgctc agcactgtga acttctacgt tccgcccgcc tacagcaaag
     1621 gggggaacgt gctgccccaa acgtcacgga gtcgctaata cccgcgactt ccagcttgct
     1681 ccttccagat ttggactacg atgttcgctc agcgagctgt gataattgtg caactgacct
     1741 ggcagtcggg caattcctgg cggagcctgg cgattgcctt gtcggagatt gcggcttcga
     1801 cgtgcaatgc cttcagccgc ttgagccgcg acagctctgc cagcccggca tcggagatcc
     1861 cgaaactgtc gacgtaaagg ctctccagcg acgagatctg cgccagtagt tggcagcccc
     1921 gatcggtgat cgcgtcgccc gagacgttca gaattcggag cctgttcagc cccgtcaggt
     1981 attccagccc gatatcgctg atcttgttgc ctcgaagttc gatgcattcc agtcgcgggc
     2041 acttggacaa ccatatcagg ttggcgtcgg taatgtccct ctcatacagg ttgatgccgc
     2101 gcaggttggg catcggaggc gggtcccgca tgctcgtgag ccgccattgc tcgtccttct
     2161 gaggggagtc actcataaac aggtccaggg cttccaattg cggcatccgt cggagaagct
     2221 cggcaaattc ggggcttgcg ccaggcgatt gcagcaccag caggtgcgga aactggcaag
     2281 cagttcccgc gtcgcccgtg aaatacagca cgtggccgaa ttcatcgaga tatcgctttt
     2341 gatattcatc cgggagccac gccggaataa cgccgcccag actgtcggat atcgacaacg
     2401 gcacgcgcct ggccgcgctc cacagcctgt gctcttcacg tcgttcgttc cgctgctgaa
     2461 cgccccaccc gatgaggacg ccgatcagcg tgaccattcc cagcagatca accaggcgca
     2521 gctgccacag cgagcgccgc cgcgagcgcc acatctgaac tagcagcgct ccgcagaaca
     2581 gaatcccaag ccaggccaac gcgtcgatca ggattgccga ccacgagaac tccacaggat
     2641 ctctccaggg cgtccagggc gaggcccgtt cccgcgagta acctgggact tccagccacg
     2701 ccttccgccg cacatagcga accggccagc cgtgctgata ctccgtcgtc agctcgaaat
     2761 gtggtccata ccggccgctg ccgtcgagcg tcggccctgt tgcttcgacc gcgggaaagt
     2821 tggcaaagac ggcgaccaca aggatcgagc cggccgcgat gaatgtgacc ggaaatggcg
     2881 gccaggccca gctggacttt gccgctgccg attccgcggc gttggaattc gtgggcgaag
     2941 cggaggtgac aatcatgtcc attgagacgt cttgggcgaa ccagcagttt tcttctccct
     3001 acgactttag ccttcctctg ccgcaggcac agcgcgatcg cgcatcggat gtttgaccgg
     3061 tgggtcttga tcgacgaaaa tctgccgcgt actttcccag tcgcagttcg gagatcgagt
     3121 aatcatgtgc atcgtcaagc tgttttcacc atttcctggt tggcccagca aagcccggga
     3181 ttggcgaagc ctgccatacc gcgtggctgc actggcggcg acaattggat tggggaccgc
     3241 gggcattttc ctgtgctttg tcgcacaggg cgcagaatcg gcgccgccca acatcgtcgt
     3301 cgtcctcgcc gacgatctcg gctatggcga cctggcctgc ttcggccatc cggtggtgaa
     3361 atcgccaaac ctcgatcggc tggcccgcga agggctgcgg ctgacgagtt gctattcgtc
     3421 ggcggcgaac tgttcgccgg cgcggtgcgg gctgatgacc ggacgcacgc cgtatcgcgt
     3481 gggaattcac aactggattc caatgctctc gccgatgcac ctcaaggccg aagaagtcac
     3541 catcgccaca ctgctgaaaa atgccggcta cagcacgtgc cacagcggca agtggcatct
     3601 caacggcatg ttcaatctcc ccggccagcc gcagcccggc gaccacggct tcgagcattg
     3661 gttcagcacg cagaacaatt gcctgccgaa ccacaaaaac ccgtacaact tcgtccgcag
     3721 tgattctgtc ggcgggccgc gcggcggtat tcccgtcggc ccgctcgaag gctacgccgc
     3781 gcagctcgtc gctgaggaag cgatcgcctg gctccgggag ggccgcgaca aaaccaaacc
     3841 cttcttcctc tatctctgct tccacgagcc gcacgagccc
//
LOCUS       DASUGB010001017         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1918348, whole genome shotgun sequence.
ACCESSION   DASUGB010001017 DASUGB010000000
VERSION     DASUGB010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918348"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFV87_19280"
     CDS             complement(<1..543)
                     /locus_tag="VFV87_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5105974.1"
                     /translation="MTATPAKPKSLGDILQEFAQHRQLMQQELQKVIVGQNDVIEQLF
                     AAIFTRGHCLLEGVPGLAKTLMVSTLARILDVSFKRIQFTPDLMPSDITGTNVLEEDE
                     TGKRNFRFVEGPVFTNILLADEINRTPPKTQAALLQAMQEREISIGRTTYDLPDPFFV
                     IATQNPIEQEGTYPLPEAQLD"
     gene            733..3087
                     /locus_tag="VFV87_19285"
     CDS             733..3087
                     /locus_tag="VFV87_19285"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5105975.1"
                     /translation="MANRICSIVLLLFLVHLARADFAGAEITAAQVKESIDSGCKYLI
                     SQQHPDGFWPEYDGEPGGLTALCTMALLNSGRSKDDPVVAKALAYLERKSNPGRTYSA
                     SLMIMVFAQADPVGKKLAITRLANWLEAHQVRDNDTKGGWTYSERGGRADSSNTQFAI
                     LALHEAERVGVKVNDQTWKLALHFWTKPGMQNGDGGYGYDVGHPSTGSMTCAAIASLI
                     IINGRLSPGDAQLVDGRLQCCGNQEESPPLDNALTWLSRHFSVQRNPNTPSWLLYYLY
                     AMERVGRMSGQRTIGGHDWYREGSEFLVAQQDATLNNSWVGNGFIENNPIVATSFALL
                     FLSKGRRPVVLGKLNHQPDALGLSSDWDHHRRAVQHLTMAVEQAWKRDLSWQTIDIRN
                     STAADLLEAPVLFLSGAESLRFTPDEKRSLKQYIEQGGFIFAEACDGNGCDGAQFDRD
                     FRALMRELFPDTELRKLPPDHAVWYAEKPVDPKHLPQDPEFWLWGLDACCRTSVVYCP
                     RSLSCYWELAHPYHSAAYAEEKKDEIEAVVNIGMNVVSYATGRELKEKLDRPQITISN
                     PGSKSPRGALVVPKLSHGGGADDAPSALNNLLTVMEKQLEMRVDYEKRLIAPSDEKLY
                     DFPVLFMHGRRSFKFSAAERKALKDYLDRGGFLFADSICANKEFADSLRAELKAIYPE
                     ADFARIPPSHAIFTAEFHGFDLPSVQLRDPQVRAEGDPLTAKIVTTTPLLEGLDIDGR
                     IAVVLSPNDISCALEKGASLECKSYIPPDAARLGANVLLYALQQ"
BASE COUNT          616 a         1019 c         1050 g          577 t
ORIGIN      
        1 gtcgagctgg gcttcgggca gcgggtaggt gccttcctgc tcgatcgggt tctgcgtagc
       61 gatgacaaaa aacgggtcgg gcaggtcgta ggtcgtgcgg ccgatgctga tctcgcgctc
      121 ctgcatcgcc tgcaagaggg ccgcctgcgt tttcggcggc gtgcggttga tttcgtcggc
      181 cagcagaatg ttcgtgaaga caggcccctc gacaaaacgg aagttgcgct tgcccgtctc
      241 gtcttcctcc agcacgtttg tgcccgtgat gtcgctgggc atcaggtcgg gtgtgaactg
      301 aatccgctta aagctcacgt ccaggattcg cgccagcgtg ctgaccatca gcgttttggc
      361 cagccccggc acgccctcca gcagacaatg cccgcgggtg aagatcgccg cgaagagctg
      421 ttcgatcacg tcgttctggc cgacgatcac cttctgcagc tcttgctgca tgagctggcg
      481 gtgctgggcg aattcctgca ggatgtcgcc cagggatttg ggtttggcgg gggtggcggt
      541 catgcgggag gggacagggg acaggggaca gggaacaggg gtcagatgtc agaggtcaga
      601 tgtcagaggt gtcagacgcc acgggcgcgg agattcctag tgtcgccgac ctgcaagtac
      661 tgagtactca gtactgagta cttggcctat gatagcgagt ggggctacca cggcgcagac
      721 ccggaggcgg cgatggccaa tcgcatttgt tcgatcgtcc tactcctttt ccttgtgcat
      781 ctcgcccgcg ccgatttcgc cggtgcggag atcacggcgg cgcaggtcaa ggaatcgatc
      841 gacagcggat gcaagtacct gatcagccag cagcatccgg acggcttctg gccggaatat
      901 gacggcgagc cgggcgggct gactgctctg tgtacgatgg ccctattgaa cagcgggcgg
      961 tcgaaggacg acccggtggt ggccaaggcc ctggcctacc tggagcggaa gtcgaacccc
     1021 ggccgcacct attccgcctc gctgatgatc atggtctttg cccaggccga cccggtcggc
     1081 aaaaagctgg cgatcacgcg gctggcgaac tggttggaag cccaccaggt ccgcgacaac
     1141 gacacgaaag ggggctggac ctacagcgag cgcggcggcc gcgccgacag cagcaatacg
     1201 caatttgcca tcctcgccct gcacgaagcc gagcgggtcg gcgtcaaagt caacgaccag
     1261 acctggaagc tcgcgcttca tttctggacc aagcccggca tgcagaacgg cgacggcggt
     1321 tacgggtacg acgtaggcca tccctcgacg ggcagcatga cctgcgccgc aatcgcctcg
     1381 ctgatcatca tcaacggccg cctcagcccg ggcgacgcgc agctcgtcga tggccgcctg
     1441 caatgctgtg gcaaccagga agaaagcccg ccactggata acgccctgac ctggttgagc
     1501 cggcatttct cggtccagcg gaacccgaac acgccaagct ggctgctcta ttacctgtat
     1561 gccatggagc gggtcggccg catgtcgggc cagcggacga tcggcggcca tgactggtac
     1621 cgcgaaggga gcgaattcct cgtcgcccag caagatgcga cgctcaacaa cagttgggtc
     1681 ggcaacggct tcatcgagaa taacccgatc gtggccacgt cgttcgctct gctgttcctc
     1741 tcgaagggcc gccggccggt cgtcctgggc aaactcaatc accagccgga tgcgctcggc
     1801 ctgtccagcg attgggacca tcatcggcgg gccgttcagc acctgacgat ggcggtcgag
     1861 caggcgtgga aacgcgatct ctcgtggcag acgatcgaca ttcgcaattc gactgccgct
     1921 gacctgctcg aagcgcccgt gctcttcctc agcggcgccg aaagtctgcg cttcacgccc
     1981 gacgagaagc ggagtctcaa gcaatacatc gagcaggggg gcttcatttt cgccgaagcc
     2041 tgcgacggca acggctgcga cggggcacag ttcgaccgcg atttccgggc cctgatgcgg
     2101 gagctgttcc ccgataccga gctccgcaag ctgccgcccg atcacgccgt ctggtatgcg
     2161 gagaagccgg ttgatcccaa gcatctgccg caggacccgg agttctggct gtggggcctg
     2221 gatgcctgct gccgcacgag cgtcgtctat tgcccgcgga gcctgtcgtg ctactgggag
     2281 ctggcccatc cgtaccactc cgccgcctac gccgaggaaa agaaggacga aatcgaagcg
     2341 gtcgtcaata tcggcatgaa cgtggtctcg tatgccaccg gccgcgagct gaaggaaaaa
     2401 ctcgaccgcc cgcagatcac gatcagcaac cccggcagca aatcgccccg cggggccttg
     2461 gtcgtgccca agctcagcca cggaggcggc gccgatgacg cgccgagcgc tcttaacaat
     2521 ctgctgaccg tgatggagaa gcagctcgaa atgcgggtcg attacgagaa gcggctgatc
     2581 gccccgagcg acgagaagct gtacgacttt ccggtcctgt tcatgcacgg ccgccgctca
     2641 ttcaagttct ccgctgccga gcggaaggcc ctgaaggact atctcgaccg cggcggtttc
     2701 ctgtttgccg actcgatttg tgcgaacaag gagttcgccg attcactgcg ggccgagctg
     2761 aaggcgatct atcccgaagc cgatttcgcc cgcatcccgc ccagccacgc gatattcacc
     2821 gccgagttcc acggcttcga tctgccgagc gtgcagcttc gcgacccgca agtgcgagcc
     2881 gagggcgacc cgctcaccgc gaagatcgtg acgacgacgc cgctcctcga gggcctcgac
     2941 atcgacgggc ggatcgcggt tgtcctctcg cccaacgaca taagttgcgc cctggaaaaa
     3001 ggggcctcac tagagtgcaa gagctatatt ccgcccgacg ctgcccgcct gggcgcgaac
     3061 gtcttgctct acgccctgca gcaatagaga gtagggcagg tatgccaagc gccgccgatg
     3121 gcagcgtgcg ctatctgccg cgcgggattg cgatcgagaa gctgcgggcg ctgaattcgc
     3181 ccgcggatgg ggaggcggtt ttggggagga atgagccaaa caactcgttc gcagttcggg
     3241 aaccgcgacc actggtcgcg gc
//
LOCUS       DASUGB010001018         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_111709, whole genome shotgun sequence.
ACCESSION   DASUGB010001018 DASUGB010000000
VERSION     DASUGB010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111709"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFV87_19290"
     CDS             <1..870
                     /locus_tag="VFV87_19290"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5105976.1"
                     /translation="LLFLRDTSVLLAAGQRQFEVRLQYQMDDSDTVLARVQNNVFQIG
                     EAKLRQRLLLMPLELRYGFSPVTQLFVNVPFGWSNGEVSFLNEDEFNNVGGIGDVSAG
                     FTRQILDGKGNCPDVLFSFSFSAPTGNSSFASSLSTPGANLGEGVWSLTGALTAIHTY
                     DPVVVFYGFGYRHRFEGFFDDPLSDVDLFVNPGSQILYRFGVGFAINPQITLSASFLG
                     QYISEPSVNGVRLAGTIREPMQVRLAATIVRGQKTKTYPYGPPGIKFIEPYVNFGLTD
                     AAIDSIIGVSVTW"
     gene            907..1656
                     /locus_tag="VFV87_19295"
     CDS             907..1656
                     /locus_tag="VFV87_19295"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX5105977.1"
                     /translation="MMRPIWTSTILAASLAALTAPAPAQQPPIQAPPTRSQPQSPRIR
                     APIRDNERTFQKGVKSWREISREHIVMQQRDYSCGAAALATLMRYHLNENVTELQLLL
                     EVVQMLTPEEMRDRVQNGLSLTDLRRVAVRTGHLATIGTLEFDKLRESKVPLVVGIVV
                     NEFDHFVVFRGVDDQYVYLADPARGHVRTPIEEFKKQWQKNAVLVVVRMDDAGEDKRS
                     PYMVTPEESSLGQTNRNFVRGQVTTKFLPYP"
     gene            complement(1711..3036)
                     /locus_tag="VFV87_19300"
     CDS             complement(1711..3036)
                     /locus_tag="VFV87_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5105978.1"
                     /translation="MTPRSTTSRRNFLQASAAAAVCSAAMPIFAAPARRPKVAAVFTE
                     LRLRSHAYNILINCMWPYLFCGRRVGPGVDVVSFYADQFPAGDMARDVSKRLAVPLFP
                     SIAEALTLGGKELAVDAVLLIGEHGEYPDNELGQKMYPRKEFFDQIVAVMRRSERFVP
                     VFNDKHLSYRWDWAKEMYDTARRLGIPLMAGSSVPLGERRPAIELPADAQIAEAVAIH
                     GGGMEVYGYHGLELLQSFVESRKGGETGISRVQLLVGEAYEEAARGGRWSAELAAAAM
                     AAERAADFQRQKRPMASAGSNQPTPDHAILVTYKDGTRGTVLRIGNTSDRWNFACRLR
                     GKDKPLATAIYNGPWGNRNLFAALTNAILHFFKTGKSPYPVERTLLAGGAIDAAMHSH
                     HSGGKPIDTPQLEFAYPARDFAPFRESGESWKVITPEVPQPVDFAPGKG"
BASE COUNT          592 a         1052 c          904 g          598 t
ORIGIN      
        1 ctgctcttcc tgcgcgacac ctccgtcctg ctcgcggcag gtcagcggca attcgaggtt
       61 cggctgcagt accagatgga tgacagcgac accgtgctcg cccgcgtgca aaacaatgtc
      121 ttccagatcg gcgaagcgaa actccgacag cggctgttgt tgatgccgct ggaactgcgt
      181 tacggcttct cgccggtgac tcagctcttc gtcaacgtgc cttttggctg gtcgaacggc
      241 gaagtttcct tcctgaacga agacgagttc aacaatgtgg gcggaattgg agatgtgagc
      301 gcgggattca ccaggcagat cctggacgga aaaggaaact gccccgatgt actcttctcc
      361 ttctccttct cggcgccgac gggcaattcc agctttgcgt catcgctctc cacgcccgga
      421 gcaaacctgg gcgaaggcgt ttggtcgctg actggcgccc tgaccgccat ccatacctac
      481 gatcccgtcg tcgtgttcta cggcttcgga taccggcacc ggttcgaagg atttttcgac
      541 gatccgctct cggacgtcga tctgttcgtc aatcccggct cgcagatcct ctatcgcttt
      601 ggcgtcggct tcgccatcaa tccgcaaatt acgctcagcg cgtcgttcct cggacagtac
      661 atcagcgagc catcggtcaa cggtgtgcgg cttgccggca ccatccgcga accgatgcag
      721 gtccgcctgg cggcaacgat tgtccgcggc caaaaaacga agacgtaccc ctacggcccg
      781 ccgggaatca agttcatcga gccgtacgtc aattttgggc tcaccgatgc cgcgatcgat
      841 tcgatcatcg gcgtcagcgt gacctggtaa gccggcctta gttccatgac gactccgcgt
      901 tgttcgatga tgcgccccat ctggacgagc acgatcctgg cggcaagcct ggcagcgctg
      961 acggcgcccg cgccggccca gcagccgccg attcaagcgc cgcccacgcg gtcccagcct
     1021 cagtcgccgc gaattcgcgc gccgatccgg gacaacgagc ggactttcca gaagggggtc
     1081 aagagctggc gcgaaatcag ccgcgagcac atcgtcatgc agcagcggga ctattcctgc
     1141 ggggctgcag ctctggcgac gttgatgcgc tatcacttga acgagaacgt caccgagctg
     1201 caattgctcc tcgaagtcgt gcagatgctc acgcccgaag agatgcgcga tcgggtgcaa
     1261 aacgggctct ccctcaccga tctgcggcgc gtcgccgtcc gcaccggcca tctggcgacg
     1321 atcggcacgc tggagttcga caaactccgc gaatcgaaag tgccgctcgt cgtcggcatc
     1381 gtcgtcaacg agttcgacca tttcgtcgtc tttcggggcg tcgatgacca gtacgtctat
     1441 ctggccgacc cggcccgcgg ccatgtgcgc acgccgatcg aagaattcaa gaagcaatgg
     1501 cagaagaacg ccgtgctcgt cgtggtgcgg atggacgacg cgggcgagga taaacgctcg
     1561 ccatacatgg tcacccccga agaatcatcg ctgggccaga ccaatcgcaa tttcgtccgc
     1621 ggccaggtga cgacgaaatt cctgccttat ccatagctcc ctcctgcgca tttttgaccg
     1681 cggtgcctgc cccgcactcg cactaatccc tcaacccttc cctggcgcga aatcgacggg
     1741 ttgcggaact tcgggcgtga tcactttcca actctcccct gactcgcgga agggggcaaa
     1801 atcccgagct ggatacgcga attcaagctg tggcgtgtca atcggcttgc cgccggagtg
     1861 gtgcgagtgc attgcggcgt cgatggcgcc gccggccaga agcgtccgct cgaccggata
     1921 gggcgacttc cccgtcttga agaagtgcaa gatcgcgttc gtcaacgccg cgaataaatt
     1981 gcggttcccc cacgggccat tgtagatcgc ggtcgccagc ggcttatcct ttccacgcag
     2041 ccggcaggcg aaattccagc ggtccgacgt attgccaatt ctcagcacgg ttccccgcgt
     2101 gccgtccttg tacgtgacga ggatcgcgtg atcgggcgtc ggctggttgc tgccggcgct
     2161 ggccatcggc cgcttctgac gctgaaaatc cgcagcccgc tcagccgcca tcgccgcggc
     2221 cgccaattcc gccgaccagc ggccgcctct tgcggcttcc tcgtaggctt cgccgacgag
     2281 aagctgcacg cggctgatgc cggtctcgcc ccccttacgc gattcgacaa aggattgcaa
     2341 cagctccaga ccgtgatagc catagacttc catccctccg ccgtgaattg cgaccgcctc
     2401 tgcgatctgc gcgtctgcag gcaattcgat cgccggacgc cgctcgccta acggcacgct
     2461 gctccccgcc atcagcggaa tccccagccg ccgggccgtg tcatacatct ccttggccca
     2521 gtcccagcga tacgagaggt gcttgtcgtt gaacacaggc acgaaccgct cggagcgccg
     2581 cattaccgcc acgatctggt cgaagaactc cttgcgcgga tacatcttct ggcccagttc
     2641 gttgtccgga tactcgccgt gttcgccgat caacagcacc gcatcgaccg ccagctcctt
     2701 cccgcccaac gtgagcgcct cggcgatgct cggaaacagc ggcacggcca gccgcttgga
     2761 aacatcgcgg gccatgtcgc ccgcgggaaa ctgatccgca tagaacgaaa cgacatcgac
     2821 tccgggcccg acccgccgcc cgcaaaacag gtacggccac atgcagttaa tcaggatgtt
     2881 gtaggcatgc gaccgcagac gcagctccgt aaacacggcc gcgaccttcg gccgcctggc
     2941 cggtgcggca aagatcggca tcgcggcgct gcacaccgcc gcggcggctg aagcctgcag
     3001 gaaattacgg cgagaagttg tggagcgtgg tgtcatggcg tgagacctca cagggatgaa
     3061 gcgctgcatc aggcgtcgag gcttccattt ctaaccccac atttataacc caccctgctc
     3121 cacctccgac tttgtgtcct tcgcag
//
LOCUS       DASUGB010001019         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_947336, whole genome shotgun sequence.
ACCESSION   DASUGB010001019 DASUGB010000000
VERSION     DASUGB010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947336"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..316
                     /locus_tag="VFV87_19305"
     CDS             86..316
                     /locus_tag="VFV87_19305"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX5105979.1"
                     /translation="MTQAALDLREQLARLPQEDRAALAKFLLESLDDEVDEDAEAAWA
                     VELERRAAAVRAGTATGRPAMQALDELRAKHS"
     gene            313..615
                     /locus_tag="VFV87_19310"
     CDS             313..615
                     /locus_tag="VFV87_19310"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5105980.1"
                     /translation="MKPATLDADAEAELDDAIAWYNRRDPGIGLELEDEVRAAISRIE
                     QNPGIGARYRNTDYRFYRVKRFPYLIYYMEVPDKVWVTAIAHGSRRPNYWRKRKPP"
     gene            788..1507
                     /locus_tag="VFV87_19315"
     CDS             788..1507
                     /locus_tag="VFV87_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5105981.1"
                     /translation="MDLSLPAALERLADLEYTSVELALFEDADQFKPSQIAADPEKAV
                     AICRNTQRLDVVAFDTRIAAEKDDHYEQFNAICKLARATKVVTITVPSGETGTPFNEE
                     IEHLRRLVDIATLHGVRVGIKSQIGRMSEDPDTVTNLCDNVKGLGLTLDPSAYIYGPY
                     QGRNYDKLMKYVYHMHLRDTNKKAFQVRVGQGEIEYGRLISLLQKQKYNRALSVDITE
                     MPEVDHVGELRKLRLLLESLL"
     gene            complement(1551..>2711)
                     /locus_tag="VFV87_19320"
     CDS             complement(1551..>2711)
                     /locus_tag="VFV87_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX5105982.1"
                     /translation="VIVHQHGCGEGSCQSGLTGAYDLHWQALARKHHCALLSPAYEQP
                     EGAECQRWCDPRNGSSAAFQRCLVDLGEKSRHSELSALPWALWGHSGGGHWAGGMVLL
                     HPERVAAAWLRSGVPLLEPNPDRSNIKPHTLPAAALKVPMMCNLGTKEGVTDKESRFA
                     GVWPANEVFFEGVRSRGGLIGVAVDPLTSHECGNQRYFAIPWLDACLAARLPKVSGEP
                     LQDMGAENVWLAPVTGAEAVPAANFAGDPLQAAWLPNESIARAWMQYVQDTALIDTTP
                     PPAPTNLRTRGNELSWEVQADLESGLASFILERDGQILAHLPEQAKNSFGRPVFQNLQ
                     YSDTPTLPLVPLRFIDATAESGRQHTYRVVAVNTAGLKSKPSEPATATETRE"
BASE COUNT          527 a          830 c          867 g          487 t
ORIGIN      
        1 ctaactaccg ctgccgcaag cgcttgaagc cgtaggaccg gctgccgcgg aatggtagaa
       61 tttgggtgac cccaggagaa ttccgatgac tcaggctgcc ctcgacctgc gcgaacaatt
      121 ggcccgcctg ccgcaggagg accgcgcggc actggcgaag tttcttctcg aatcgctgga
      181 cgatgaagtg gatgaggacg ccgaggcggc gtgggcggtc gaattggagc ggcgggcggc
      241 tgcggtccgc gccggaacgg cgacaggtcg cccggcgatg caggcattgg acgagctgcg
      301 agcgaaacac tcgtgaagcc tgcaactctt gatgccgacg ccgaagcgga gttggacgac
      361 gccattgcgt ggtacaatcg gcgggatccg ggcatcggcc tggaacttga agacgaggtc
      421 cgtgcggcga tttcgcgaat cgagcagaac cctggaattg gggcgcgata ccgcaacacc
      481 gactatcgat tctaccgcgt gaagcggttt ccctatttga tctattatat ggaggtgcca
      541 gacaaggtct gggtcactgc tattgcccat ggaagccggc ggcccaatta ctggcggaag
      601 cgaaaaccgc cttgaaggct tcgcccacaa gcccgcggct gaggttgcct ccggccgctt
      661 gttcccggta agctacgcat tctagggact tgaattctag ggacttgggc tgctagggac
      721 ttggcccgca ctgtccgccg acaaaggagt ggttctcgtg ctagttgcgg ctacgacgga
      781 atgtttcttg gatctttcgc tgccggcggc tttggagcgg ctggcggact tggaatacac
      841 cagcgtcgag ctggccctgt ttgaggacgc cgaccagttc aagccctcgc agatcgcggc
      901 cgacccggaa aaggccgtcg ccatctgccg caacacgcag cggcttgatg ttgtcgcgtt
      961 cgatacgcgg atcgccgcgg aaaaagatga tcattacgaa cagttcaacg cgatctgcaa
     1021 gctggcgcgg gccacaaagg tcgtgacgat cacggttccc tccggcgaga cggggacccc
     1081 cttcaatgaa gagatcgagc acctgcggcg gctggtcgat atcgccacgt tgcacggcgt
     1141 gcgggtgggt atcaagagcc agatcggccg catgtcggaa gacccggaca cggtcacgaa
     1201 cctctgcgac aacgtgaagg gcctcggcct gacgcttgac ccgagcgcct atatctacgg
     1261 gccgtaccag ggccgcaatt acgacaagct gatgaagtac gtctatcaca tgcacctccg
     1321 cgacacgaac aagaaggcct tccaggtgcg cgtcggtcag ggcgaaatcg agtacggccg
     1381 cctcatcagc ctcttgcaga agcagaaata caaccgcgcc ctcagcgtcg acatcaccga
     1441 gatgcccgag gtcgatcacg tcggcgagct gcgcaagctg cggctgctgc tggaaagctt
     1501 gctgtgagga gtgacgagtc cgaggaatct gtcgagacct tggcacccgg tcactcgcgc
     1561 gtctcggtcg cggtggccgg ctcgctgggc ttcgacttca ggcccgccgt attcacagcc
     1621 acgacgcgat aggtgtgctg ccggccggat tcggccgttg cgtcgatgaa gcgcagcgga
     1681 acgagcggca gggtgggcgt gtcgctgtat tggagattct gaaacacggg acggccgaac
     1741 gagttcttag cttgctcggg aaggtgcgcg aggatctggc cgtcgcgctc gaggatgaag
     1801 ctggccaatc cgctctcgag atcggcctga acctcccaag acagctcatt gccacgggtg
     1861 cgcaggttcg tcggcgcggg aggtggagtc gtatcaatca gtgcggtgtc ttggacatac
     1921 tgcatccagg cccgcgcgat cgactcgttt gggagccagg cggcctgcaa aggatcacca
     1981 gcgaagtttg cggccggcac agcttctgcg ccagtgacgg gtgcgagcca gacgttttcc
     2041 gcccccatgt cctgaagggg ctcgccactg accttcggca agcgggccgc cagacacgca
     2101 tcgagccacg gaatggcaaa gtacctctga ttgccgcact cgtggctcgt gagcggatca
     2161 accgccacac cgatcaggcc tccacgcgaa cgcactccct caaagaacac ttcgttcgcc
     2221 ggccatacgc cggcaaaacg actttccttg tcggttacgc cttccttcgt ccccaggttg
     2281 cacatcatgg gcactttcag ggcggctgcg ggcagcgtgt gtggtttgat attcgagcgg
     2341 tccggattcg gctccagcag cggaacgccc gaacgcagcc aggcggcggc gacacgctct
     2401 gggtgcagca gcaccatccc gccggcccag tggccgccgc cgctgtgacc ccataaggcc
     2461 cacggcaggg cggataactc cgaatggcgc gatttctctc cgagatcgac caggcatcgc
     2521 tgaaacgcgg cactggatcc gtttcgcgga tcgcaccacc tctgacattc cgccccttca
     2581 ggctgctcgt aagcgggaga gagcagcgcg cagtggtgct tcctcgccaa tgcctgccag
     2641 tgcaggtcgt aagccccggt caaacccgac tggcatgatc cctcgccgca gccgtgctga
     2701 tgcacgatga c
//
LOCUS       DASUGB010001020         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1363331, whole genome shotgun sequence.
ACCESSION   DASUGB010001020 DASUGB010000000
VERSION     DASUGB010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363331"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1721
                     /locus_tag="VFV87_19325"
     CDS             81..1721
                     /locus_tag="VFV87_19325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5105983.1"
                     /translation="MLVEWNTLGLLRPEIILVAAATTIFVGGAFAKSRAWWALFALIS
                     YVAAGAALASYGPPWAEGSTLSGPVTIDPLSLSLRWLAVLMGLVFTLVATRLADPEIA
                     SEYVGSLMLVATGVMLTASANELVLLFLGLELISIPTYVLLFLGRRDRAAGEATMKYF
                     YLSILSSALLLYGFSFLYGIGGTTLIAGTAAHPGIREAFLPPLSLGEGRGEDSESSAG
                     DASPRPNPLPKGEGTNPLAPLAPLAVVLILAGLGFKLAIAPLQFYAPDVYQGTTNANA
                     GLLAVAPKIAGVAGLVRLLVVAMPSSAEFAWQLALILSILTMTIGNICALWQSNVRRL
                     MAYSSIAHGGYLLIGLAAAAGAISAGQPLASGGVTAMLFYVAVYALATMGTFAALAYL
                     GSPRRELDGVEQLAGLSKSQPVIAGAIAVFMFSLAGIPPLAGFWGKLTLFGSAVELAT
                     AGTPAGMSLWFTILVVIGALNAAVAAAYYLRIVAVMYFQPAAAAPSAAGGRTAHFAAL
                     LLAVLVIAAGILPGRLLDAAARSEIMLQSDAPQVAATP"
     gene            1887..2393
                     /locus_tag="VFV87_19330"
     CDS             1887..2393
                     /locus_tag="VFV87_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105984.1"
                     /translation="MNIARQFSLLFVMVLVGAPLAAQPLGEPDRGQPGDEMIQGYLAR
                     ESAKIAAKFSEDIQSLEAWQARRPQYVEEYYYMLGLSPRPEKTPLKATITGTFKDDDY
                     EVDMLHYQSRPRLYVTANLYRPAESKAGENCPACSTSAVTRAVGAAATRSPFSRTASG
                     WPATAMSA"
     gene            2291..>3505
                     /locus_tag="VFV87_19335"
     CDS             2291..>3505
                     /locus_tag="VFV87_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5105985.1"
                     /translation="MLYVCGHSGRGRSGNKVAFQSHGIWLARHGYVCLMVDTLQLGEI
                     AAFHHGTYNLNRWWWHSRGYTPAGVEAWNGVRGIDYLLSRDDVDPERIAVTGISGGGA
                     ATFWVAAADERVKVAIPVSGMADLESYVPSRVINGHCDCMFLYNTFQWPWTRIAGLVA
                     PRPLMFVNSDDDRIFPMDANERISNRLERLYSLYGESDRFETIVSVGGHAYRQDLREA
                     IFGFLNAQLKGDARPVTDTEIDVVSEGRDPGPYPIPIEKLRVFPTDADIPADELNTKI
                     DEHFVPLASVAAPQAGEFDSWKKNLLAELRRVSFGYFPETIPAAKLLDNAAERLETEG
                     GVEVRIRDSGTNANKLGPILLVVLNEEEAGKTPDWLKGVKKVDHLVVFCEPRGIGESR
                     WTRKNGPNYVERS"
BASE COUNT          568 a         1176 c         1102 g          659 t
ORIGIN      
        1 tcgattccgg ccggcgccgg ttccagaaga tggcttcttg gcggaatcga ctcccgaccc
       61 cggaatagcg gagaccgctc gtgctcgttg aatggaacac ccttggcctc ctgcggccgg
      121 aaatcatcct ggtcgccgcg gccacgacga tcttcgtcgg cggggccttc gccaagagcc
      181 gggcctggtg ggcgctgttc gcgctgattt cttatgtggc ggccggcgcg gcgctggcgt
      241 cgtacggccc tccgtgggca gaaggcagca cgctgagcgg accggtcaca atcgatccgc
      301 taagcctcag tctccgctgg ctggccgtcc tgatggggct ggtcttcacg ctggtcgcga
      361 cgcgcctggc agatcccgag atcgccagcg agtatgtcgg cagcctgatg ctcgtcgcca
      421 cgggcgtaat gctcaccgcc tcggccaacg agttggtgct gctgttcctc ggactggagc
      481 tgatctcgat tccgacgtac gtcctcctct tcctcggccg ccgcgatcgg gccgcgggcg
      541 aggcgacgat gaaatacttc tacctgagca tcctgtcatc cgctttgctg ctgtacggct
      601 tcagcttcct gtacggcatc ggcgggacga cgctgatcgc gggaactgcg gcacacccgg
      661 gtattcgcga agcgttcctt ccccctctct ctctgggaga gggccggggt gaggattccg
      721 aatcatcggc tggcgatgcg agccctcgcc ctaaccctct cccaaaggga gaggggacaa
      781 atccgctcgc gcctctggcg ccgctggcgg ttgtgctgat tctcgccggg cttggcttca
      841 agctggccat cgccccgctg cagttttatg ccccagacgt ctatcagggc accaccaacg
      901 ccaacgcggg gctcctggcc gtcgccccaa agatcgccgg cgtcgcgggg ctcgtgcgct
      961 tgctcgttgt ggcgatgcct tcctcggctg agttcgcctg gcagctcgcc ctgatcctct
     1021 cgatcctgac gatgacgatc ggcaacatct gcgccctgtg gcagtcgaac gtccggcggc
     1081 tgatggccta ttcgtcgatc gcgcacggcg gctacctcct gatcggcctg gcagccgccg
     1141 ccggtgcgat ttccgccggc cagccgctgg ctagcggcgg cgtgacggcg atgctctttt
     1201 acgtcgccgt ctatgctctg gcgacgatgg gcacgttcgc cgctctggcc tacctcggct
     1261 cgccgcggcg cgagctcgac ggcgtcgagc agctcgcggg cctcagcaaa tcgcagccgg
     1321 tgatcgccgg cgcgatcgcc gtcttcatgt tctccctggc cggcattccg cccttggccg
     1381 ggttctgggg caagctgacc ctcttcggca gcgcggtcga attggccacg gctggcacgc
     1441 ccgccggcat gtcgctctgg ttcaccatcc tggtcgtgat cggcgcgctc aacgccgccg
     1501 tcgccgcggc ctattacctg cgcatcgtgg ccgtcatgta cttccagccc gctgccgccg
     1561 ccccatccgc tgccggcggc cgcaccgccc acttcgccgc gctcctgctg gcggtgctcg
     1621 tcatcgcggc cggaattctg cccggccgct tgctcgacgc tgccgccagg tcagaaatca
     1681 tgcttcagtc ggatgcaccg caggttgcag cgacaccgta gcggcgattg tccgacttca
     1741 ccttcggcgc gatattgctc gagttcaact ccgcgaagca gcgcagtgcg cctcgagcgc
     1801 ggttcaatcg acgcaaatca ctatgcgccg gtgttccagc cgcctatgat cgagctcaac
     1861 ttcgagtctc gtgcggagtt cctcgcatga atatcgcccg tcaattctcg cttctcttcg
     1921 tcatggtctt ggtgggcgct ccgcttgccg ctcagccgct gggcgagccg gaccgcggcc
     1981 agccggggga cgagatgatc cagggatatc ttgcgcgcga atcggcgaaa atcgcggcga
     2041 agttcagcga ggacatccag tcgctcgagg cctggcaggc caggcggccg caatatgtgg
     2101 aagagtatta ctacatgctc ggcctctcgc cgcggccgga gaaaacgccg ctgaaggcga
     2161 cgatcacggg cacgtttaag gacgacgact atgaagtgga catgctccac tatcagagcc
     2221 ggccgcggct gtatgtgacg gcgaatcttt accggcccgc ggagtcgaaa gcgggcgaaa
     2281 actgcccggc gtgctctacg tctgcggtca ctcgggccgt gggcgcagcg gcaacaaggt
     2341 cgcctttcag tcgcacggca tctggctggc ccgccacggc tatgtctgcc tgatggtcga
     2401 tacgctacag ctcggcgaga tcgccgcctt ccatcacggc acctacaacc tcaaccgctg
     2461 gtggtggcat tcgcggggct acacgccggc cggagttgaa gcgtggaacg gtgtccgcgg
     2521 catcgactac ctcctcagcc gcgacgatgt cgatcccgag cgaatcgccg tcaccggcat
     2581 cagcggcggc ggagcggcca ccttctgggt cgccgctgcc gacgagcggg tgaaggtcgc
     2641 cattcctgtc agcggcatgg cggacctcga atcctacgtc cccagccgcg tgatcaacgg
     2701 ccactgcgac tgcatgttcc tttacaacac gttccagtgg ccctggacgc ggattgccgg
     2761 cctggtcgcc ccgcgtccgc tgatgttcgt caatagcgac gacgaccgca tctttccgat
     2821 ggacgccaac gagcggatca gcaatcgcct cgaacggctt tacagcctat acggcgagag
     2881 tgaccggttc gagacgatcg tcagcgtcgg cgggcacgcg tatcgccagg atctgcgcga
     2941 ggccatcttc ggcttcctca acgcccagct caaaggggac gcccgcccgg tgactgacac
     3001 cgagatcgac gtcgtcagcg aaggccgcga ccccggcccc tatcccatcc cgatcgaaaa
     3061 actccgcgtc ttccccaccg acgccgacat tcccgccgac gagctgaaca cgaagatcga
     3121 cgagcacttc gtgccgctcg cgtccgtcgc cgcgccgcaa gcgggcgagt ttgactcctg
     3181 gaaaaagaac ctgctcgccg agctgcggag ggtcagcttc ggttatttcc cggagacgat
     3241 tccggcagcg aagctgcttg ataatgcagc cgaacggttg gagacggagg gaggcgttga
     3301 agtccgtatt cgggacagcg gcaccaacgc gaataagctt ggcccgattc tgctggtagt
     3361 gctcaatgaa gaggaagccg gcaaaacgcc ggactggctg aaaggcgtga agaaagttga
     3421 ccaccttgtc gttttttgcg agccgcgggg catcggcgag tcgcgatgga ctcggaagaa
     3481 cggaccgaac tacgtcgaac gttcg
//
LOCUS       DASUGB010001021         5931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1918709, whole genome shotgun sequence.
ACCESSION   DASUGB010001021 DASUGB010000000
VERSION     DASUGB010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918709"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFV87_19340"
     CDS             <1..974
                     /locus_tag="VFV87_19340"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEX5105986.1"
                     /translation="WLLAAYISIGLIRPLLGPPRAPATPTTESSEAEFLERPEAAPLY
                     VPIRNLLLAVIGLFAVYLVFEFRTLWFREFPQGFYYSGYAHEGAAWLTAALALATLVL
                     SLIFRGRVLRDPRLARLKHLAWIWSALNLLLAVTVYHRMSIYIDFNGMTRMRTIGLFG
                     ITTVVAGFVLVLWKIHQNRDFLWLIHQQLWALAIAIYLYALTPVDALVHRYNVRQILA
                     GDIAPCVQISVHPNSAEGYLQLQPLTNCRDPIIRDGIRALLAQREIAAEERAAERAQL
                     GWTTYQLSDRLLLDQLRENRSEWQEFSDESARTAALERFHKYAYQWY"
     gene            complement(971..1378)
                     /locus_tag="VFV87_19345"
     CDS             complement(971..1378)
                     /locus_tag="VFV87_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105987.1"
                     /translation="MYIAVVAVIERGERQAAALGFVLWMAVYVSLLFLQPDTRRFPTE
                     KLLTPVFESVVTRTWVDDRTGAVIPDYDPEKPSRLVSEKTDPEHRWFMNIGHVLWALL
                     LGYIGSRIATWICARRIAIRTDAQNRTSQADVN"
     gene            complement(1527..1919)
                     /locus_tag="VFV87_19350"
     CDS             complement(1527..1919)
                     /locus_tag="VFV87_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX5105988.1"
                     /translation="MRTLYSLLVLVFFGSWFFLPAPAAAEEGKVLRHIVMYQFKPETT
                     PAQIQEVIDAFAGLPKKIDTIIDFEAGANVSQEGKSEGFTHVFVVTFKNEADLAAYLV
                     HPAHEAYVKVVKDKREKVVVFDYWADAK"
     gene            complement(1932..2474)
                     /locus_tag="VFV87_19355"
     CDS             complement(1932..2474)
                     /locus_tag="VFV87_19355"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX5105989.1"
                     /translation="MPKREPTIWTVGHSTRPIEEFLELLAAAGIELLADVRRFPASRR
                     HPQFNREALIQALAETRIGYEHFPGLGGRRNNRLPDSPNRAWRVQAFNAYADHVQSGE
                     FQADFDRLIELAETRRTAIMCAEALPQQCHRRIIADVLAAHGWTVKHLLTPKRIEDHR
                     LTEFARVEGHAVTYPQATLF"
     gene            complement(2484..3887)
                     /locus_tag="VFV87_19360"
     CDS             complement(2484..3887)
                     /locus_tag="VFV87_19360"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5105990.1"
                     /translation="MAGLTRHSHQHAGATLEMPLAPPGRDTIRIGSRRWFLQTGLAGL
                     AGVSLPAILAGRAMAGNRNAAEGVPYKSQKSVIMFWLSGGPSHIDMWDPKPDAPQEIR
                     SPFATLATKVPGTFFTEHLPLQASIAEKLSVIRSVDCSASNHTPITMQAGNPLARRTD
                     DGRDGAGYPSMGSIAAKFRGSNDPDLPAFVGLAPSWAADVYGAGNLSSQFEPVKGLEL
                     AGKFALPGGIEAPRLADRNQLRSEFDRLKATLDRTTTMQRLDRYTQQAYDMVLSGKVQ
                     RAFDIASERNELRETYGKNSVGEKALLARRLVESGVTYVLISGAWGYFDHHGDNVRWG
                     GIEKGLKPALPDADRALWALVNDLEARGLLDQTLVMMLGEFGRSPVINKEAGRDHWTA
                     VMSMVMAGGGLPHGQVIGSTDSRGGEVASATVRPQDLAATTFKHLGIDLDAHWVNVQG
                     RPISIIQEGGRPIPELC"
     gene            complement(3963..4238)
                     /locus_tag="VFV87_19365"
     CDS             complement(3963..4238)
                     /locus_tag="VFV87_19365"
                     /inference="COORDINATES: protein motif:HMM:NF045445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105991.1"
                     /translation="MNDASDLYNLLQAAAQVLLRCFVLGFAFLLFWFAAFSLGKEWMY
                     RSASQFDLSPHEINLIHYCGMALVKGWVIVFFLFPYIAIRLVMRKRT"
     gene            4350..5354
                     /gene="pdxA"
                     /locus_tag="VFV87_19370"
     CDS             4350..5354
                     /gene="pdxA"
                     /locus_tag="VFV87_19370"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5105992.1"
                     /translation="MSSHSLQRLAVTMGDPAGIGPEVCLHLLANAEIAGQCVPVVIGD
                     AAVLRRVAEAARLPLRAPVIDREQWAASWAAIDEPTILDLRATDAAGVVAGQVSATTG
                     RAAFTYIERAIEAALARQVVAVSTAPINKEALHAAGIKYPGHTEIFADRTSAPRSCMM
                     QYSEEITCTFVTVHVGYRDVPRLLTKERILDVIELTAEGLARLRGRKPQLVVCGLNPH
                     AGEHGLFGDREEERIIVPAIETARARGIAIEGPLPPDTAFLPWKRKATDAFICMYHDQ
                     GHIPVKALAFDSAVNTTLGLPIVRTSVDHGTACDIAWQGKANPSSIFAAVRLALRLAG
                     "
     gene            5360..>5931
                     /locus_tag="VFV87_19375"
     CDS             5360..>5931
                     /locus_tag="VFV87_19375"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX5105993.1"
                     /translation="MAIVIIADDLSGAAELAGIAHAHGYVAEVHRDFDAASDAEVIAV
                     DTDSRGLLPEAAADRVRQAAREILAAKPAWLFKKVDSLLRGNVRAEIEALLDATGQGQ
                     AVLIPANPSRGRTIVRGELRIDGVPLHETALAGEPQHPRRTSRVAELISGDSLPTESI
                     DAPDVATAEELLHHAASYDDTTLAAGAADF"
BASE COUNT         1088 a         1905 c         1854 g         1084 t
ORIGIN      
        1 tctggctgct ggcggcttat atcagcatcg gcctgatccg cccgctactc ggcccgccac
       61 gagccccagc gacgcccacg accgagtcca gcgaagccga attcctcgaa cgccccgagg
      121 ccgccccgct ctacgtcccg atccgcaacc tgctcctcgc ggtgatcggc ctcttcgccg
      181 tctatctagt cttcgaattc cggacgcttt ggttccgcga attcccacag ggcttctact
      241 actccggcta cgcccacgaa ggggcggcct ggctcaccgc ggccctggcc ctggcgacgc
      301 tcgtcctctc gctcatcttc cgcggccgcg tgctccgcga cccgcgcctg gcccgcctca
      361 agcacctcgc ctggatctgg tcggccttga acctgctgct ggcggtcacc gtttatcacc
      421 gcatgtcgat ctacatcgac ttcaacggca tgacccggat gcggacgatc ggcctgttcg
      481 gcatcaccac ggtcgtcgcc ggattcgtgc tcgtgctgtg gaagattcac cagaatcgcg
      541 atttcctgtg gctcatccac cagcagcttt gggccctggc gatcgcgatt tacctctacg
      601 ccctgacgcc cgtcgatgcc ctcgtccacc ggtacaacgt gcggcaaatc ctggcgggcg
      661 acatcgcccc ctgcgtgcag atcagcgtcc atcccaacag cgccgaaggc tacttgcagc
      721 ttcagccgct gaccaactgc cgcgacccga tcatccgcga cggcatacgg gcgctcttgg
      781 cccagcggga aatcgcagcc gaagagcgcg ccgccgagcg tgcccagctc ggctggacga
      841 cgtatcagct cagcgaccgg ctgctattgg atcagcttcg cgagaaccgg agcgagtggc
      901 aggaattctc cgatgaatcc gcccgaaccg cagcgttgga gcggttccac aaatatgcgt
      961 atcagtggta ctagttcaca tcagcctgac ttgttcggtt ttgggcatca gttcggatcg
     1021 ctattcgccg cgcgcagatc cacgtggcga ttcggcttcc gatatatccc aacagcaatg
     1081 cccacagaac gtggccgatg ttcatgaacc agcggtgttc ggggtccgtt ttctcactga
     1141 ccaggcgaga tggtttctcg ggatcgtagt caggaatgac tgctccggtt cgatcgtcaa
     1201 cccatgtccg cgtgacgact gactcgaaaa ccggggtcag caacttctcc gtcggaaacc
     1261 gcctggtgtc tggctgcaag aatagtagcg acacataaac tgccatccac aggacaaacc
     1321 ccaatgctgc cgcctgacgc tcgccgcgtt caataaccgc gacgacggcg atgtacatga
     1381 agatcaaaaa cgccaagccc aggagtatga tccaccacaa ctcccccgcg tacttcagag
     1441 ctgcgcagct caggcaaacg aacgctacaa gcagagccag ctctcggata ctgaatcgaa
     1501 acatcgccgc actttttcca atcgcatcac ttggcatccg cccaatagtc gaacactacc
     1561 accttctccc gcttgtcctt gacgactttc acgtacgctt cgtgggcggg gtggaccagg
     1621 taggcggcga ggtcggcttc gttcttgaac gtaacgacga agacgtgcgt gaagccttcg
     1681 gatttgcctt cctggctcac gttcgccccg gcttcgaagt cgatgatcgt gtcgatcttc
     1741 ttgggcaggc cggcgaaggc atcgattacc tcctgaatct gggcgggcgt ggtttccggc
     1801 ttgaactggt acatcacgat gtggcggagc actttcccct cctcggcagc cgcgggcgcg
     1861 ggcaggaaga accaagaacc gaagaacacg agaaccaaga gggaatacag cgtacgcatg
     1921 ggaaggggcc tttagaagag tgtggcttgc gggtaggtga cggcgtggcc ctcgacgcgg
     1981 gcaaattcgg tcagcctatg atcctcgatc cgcttcgggg tcagcaagtg cttgaccgtc
     2041 cagccgtggg cggcgagcac gtcggcgatg atccgccgat ggcactgctg cggcagggct
     2101 tcggcacaca tgatggccgt ccgcctcgtc tccgccaatt cgattagccg gtcgaaatcg
     2161 gcctggaact cgcctgattg cacgtgatcg gcataggcgt tgaaggcttg gacgcgccag
     2221 gccctattcg gcgaatccgg cagccgatta tttcgccggc cgccgaggcc gggaaagtgc
     2281 tcgtagccga tcctcgtttc ggccagcgcc tggatcagcg cttcccgatt gaactgcgga
     2341 tgccgccgcg aagccggaaa ccgccgcaca tcggccagca gttcaatccc ggctgccgcc
     2401 agcagttcca gaaactcctc aatcggccgg gtcgaatggc cgaccgtcca gatcgttggc
     2461 tcgcgcttgg gcattccctg agcttagcat agctccggaa tcggtcggcc gccttcctgg
     2521 atgattgaga tgggccgtcc ctggacgttg acccagtggg cgtcgagatc gattcccagg
     2581 tgcttgaacg tcgtggcggc cagatcttgc gggcggaccg tcgcgctggc gacttcgccg
     2641 ccgcgggaat cggtcgagcc gatcacctgg ccgtgcggca ggccgccgcc ggccatgacc
     2701 atcgacatga ccgcggtcca atggtcgcgg cccgcctctt tattgatcac gggcgagcgc
     2761 ccgaactcgc cgagcatcat gaccagcgtc tgatccagca ggccgcgggc ttccaggtcg
     2821 ttgaccaagg cccacagcgc ccgatcggca tcaggcagcg cgggcttgag ccccttttcg
     2881 atgccgcccc agcggacatt atcgccgtgg tggtcgaagt agccccacgc gccgctgatc
     2941 aggacgtagg tcacgccgct ttccaccagc cggcgggcca gcagggcctt ctcgccgacg
     3001 ctgttcttgc cgtacgtttc acgcagctca ttcctttcgc tcgcgatgtc gaacgctctt
     3061 tgcactttgc ccgagaggac catgtcgtag gcttgctgcg tgtaacgatc gagccgctgc
     3121 atggtggtgg tgcggtcgag cgtcgccttc agacggtcga actcgctgcg aagctggttg
     3181 cgatcggcca ggcgcggcgc ctcaatgccg ccgggcaggg cgaattttcc ggcgagttcc
     3241 aagcccttga ccggctcgaa ctggctgcta aggttgccgg cgccatagac atcggcggcc
     3301 caggaagggg ccaggccgac aaacgctggc aaatcgggat cgttgctgcc gcggaatttc
     3361 gctgcgattg aacccatcga ggggtagccc gctccgtcgc gtccatcatc agtgcggcgg
     3421 gccagcgggt tgccggcttg catggtgatc ggcgtgtgat tgctggccga gcaatcaacc
     3481 gagcggataa cgctcagctt ttcggcgatc gaagcctgca gcggcagatg ctcggtgaag
     3541 aaggttcccg gcaccttggt ggcaagcgtg gcgaacgggc tgcggatttc ctgcggggcg
     3601 tccggcttgg gatcccacat gtcgatatgg ctcggcccgc ctgagagcca gaacatgatg
     3661 accgactttt gcgacttgta gggaacgccc tctgcggcgt tccgattgcc cgccatcgcc
     3721 cgtccggcga gaatcgcggg cagagaaacc ccggccaggc ccgccaggcc cgtctgcaag
     3781 aaccagcggc gcgagccgat gcggatcgtg tcgcgacctg gcggcgcgag cggcatttcg
     3841 agcgtagcgc ccgcgtgctg gtgcgaatgc ctggtcaagc cggccatgcg gaactccttt
     3901 cgggaccgca tcttagccgc aaccgatcca cgaatgcaaa tctattctca aagccaagtg
     3961 gctcaagttc gctttcgcat gaccaggcgg atcgcaatgt acggaaacaa gaagaaaaca
     4021 atgacccaac ccttgaccag cgccatgccg caatagtgga tcaggttgat ctcgtgcggc
     4081 gagaggtcga attgcgatgc gctgcgatac atccattctt ttcccagaga aaacgcggca
     4141 aaccagaaca gcagaaacgc aaagcccagc acaaaacaac ggagcagcac ctgggccgcc
     4201 gcttggagca ggttgtagag atcggatgcg tcgttcatgg cgagcgcctc gacaaaagcc
     4261 acggaaatga aagatattag aatcgtacgc aatcggatca cgaaaaggat gccgggaatt
     4321 ccaccccaag cgcactttgc ctcacctcga tgagcagcca ttcacttcaa cgccttgccg
     4381 tcacgatggg cgatcctgcc gggatagggc cggaggtctg cctgcacctc ttggcgaatg
     4441 ccgaaatcgc cggccagtgc gtgccggtcg tcattggtga tgcggccgtc ttgcgccggg
     4501 tggcggaggc cgctcggctg ccgcttcgcg cgccggtgat cgaccgcgag cagtgggcgg
     4561 ccagttgggc ggccatcgac gagccgacaa ttctcgatct gcgggcgacc gacgcggccg
     4621 gcgtcgtcgc gggccaggtg agtgcgacca cgggacgggc ggccttcacg tacattgagc
     4681 gggcgattga agcggcgctg gcccggcaag tcgtcgccgt ctcgaccgca ccgatcaaca
     4741 aggaggcgct gcacgcggcg ggaatcaaat atccgggtca tacggagatc tttgccgacc
     4801 ggacgagcgc cccgcggtcg tgcatgatgc aatactcgga ggagatcact tgcacgttcg
     4861 tgacggtaca cgtcggctat cgcgatgtgc cgcgactgtt gacgaaggag cggattctcg
     4921 acgtgatcga gctgacggca gagggcctgg cgcgtctgcg ggggcggaag ccccagctcg
     4981 tcgtctgcgg ccttaatccg cacgccggcg agcacggcct gtttggcgac cgcgaggaag
     5041 agcggattat cgttccggcg atcgagacgg cccgcgcgcg cgggatcgct atcgaaggcc
     5101 cgctgccgcc ggacacggcg tttctgccgt ggaaacggaa agcgacggac gccttcatct
     5161 gcatgtatca cgatcaggga catattccgg tgaaggccct agccttcgat tcggccgtga
     5221 acaccacgct gggcctgccg atcgtccgca ccagcgtcga tcacggcacc gcctgcgata
     5281 tcgcctggca aggcaaagcc aaccccagca gcatcttcgc cgccgtgcgg ctggcgctgc
     5341 gcctggcggg ctgacgagta tggcgatcgt catcatcgcc gacgacctga gcggagcggc
     5401 ggagctggcc ggcatcgccc atgcgcacgg ctatgtggcg gaagtgcacc gggatttcga
     5461 cgcggcgagc gacgcggagg tgattgccgt cgataccgac tcgcgcggcc tgctgccgga
     5521 ggcggcggcg gatcgcgtgc ggcaggcggc tcgcgagatt ctcgcggcca agccggcctg
     5581 gctgttcaag aaggtcgatt ccctgttgcg gggcaatgtg cgggcggaga tcgaagcgct
     5641 gctggacgcc actgggcaag ggcaggccgt gctgattccc gcgaatcctt cccgcggtcg
     5701 cacgatcgtc cgtggcgaat tgcggatcga cggcgtgccg ctgcacgaaa cggcgctggc
     5761 tggcgaaccg cagcacccgc gccgcacgtc gcgcgtcgcc gaactgattt ctggcgattc
     5821 tttgccgaca gagtcgatcg acgctcccga tgtcgccacg gcggaagagc tgcttcatca
     5881 tgccgcgagt tacgatgaca cgacgctggc ggccggcgca gccgatttct t
//
LOCUS       DASUGB010001022         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1085964, whole genome shotgun sequence.
ACCESSION   DASUGB010001022 DASUGB010000000
VERSION     DASUGB010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085964"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..900)
                     /locus_tag="VFV87_19380"
     CDS             complement(7..900)
                     /locus_tag="VFV87_19380"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5105994.1"
                     /translation="MAGAWLASVGGCRALRCEQAQDEALASARQLSLHAMELQEHGRW
                     EQSEALFAAAVQQCPADERARCGYAQALWQRGARDQAVSHMEEAVRLSGHDPERLVQL
                     GEMYLFRGELQRAGQQADRAIAANSQLAAAWALRAQVEQAEGRREEALAKFHRALSLR
                     EQYPEVQLAIADIYAQDGRPQRALGTLQALAANYPPAQVPADVLIREGFALRDLGRFQ
                     DAAIALAQAAERGNASAELYYELARVQLRAGDTSAARQSALAALQRDPSHTASQALAR
                     DLAPREGIIAAATPESPSRLY"
     gene            1272..1715
                     /locus_tag="VFV87_19385"
     CDS             1272..1715
                     /locus_tag="VFV87_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105995.1"
                     /translation="MPALFERFGVKILYPENWSLMDLEVEDWPRSVTIQSQDTGFWSL
                     HVYPPQQEAEPILEELVSAIGEGFSDVEVLPATEQFGETSTAGVDLAFFYLDLLVEAK
                     IRYVHTPSAMLVWHYQAESREFERIEPVFQAIATSLLRTQVAIKE"
     gene            complement(1758..2378)
                     /locus_tag="VFV87_19390"
     CDS             complement(1758..2378)
                     /locus_tag="VFV87_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105996.1"
                     /translation="MARLGQPPLPRIGPLYADADATELITFPELDPNGAQPGMRYWGT
                     WNSSWGVRPDWPSGSRRVFFYLKPCPALEPLLKAIPELGVASIVYVPELPHERVKAMT
                     GSGALVLRGPADLRHVLQSCDLAVLHGTHGALTEALLAGTPTLNFPLNLEQLLHSPPD
                     KHPSSLCIEPPPSSVKHFLRPHHHFAAPRPGRSLSFAACLRTVGFP"
     gene            complement(2887..3594)
                     /gene="queC"
                     /locus_tag="VFV87_19395"
     CDS             complement(2887..3594)
                     /gene="queC"
                     /locus_tag="VFV87_19395"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5105997.1"
                     /translation="MSASKPSAVVLLSGGLDSATVLAIAKSAGYEPHALSFRYGQRHA
                     VELAAAERVAKSQGTASHRIVEIDLRQFGGSALTSDLAVPKKESADEIGSDIPITYVP
                     ARNTIFLSFAIAFAEVTGSSDIFLGVNALDYSGYPDCRPEYIEAFERMANLATKAAVE
                     GTQRVKIHTPLIQLTKAQIIRRGLDLGVDYSLTLSCYDPDPAGLACGHCDSCLLRLKG
                     FAENGATDPIRYAATTK"
     gene            3637..4653
                     /locus_tag="VFV87_19400"
     CDS             3637..4653
                     /locus_tag="VFV87_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5105998.1"
                     /translation="MSRNEQLIRQHKLLQLLENTRIGRTLEELRDDLVDQLGLSALHI
                     KSVKRDLEALEAAGFRIEAQHLQRGKVWRLAPHSKSAVKVQPSATELIALSIGRDLLY
                     PLAGTPFWQGIETFWNKLKEEMPSGVWKHYEAYRQVLYVRGMPTKSYARQHGMLGTIH
                     RAIVERRLLSVEYEPPGKEPQRRLIEPYAVVFYQSSLYIVAAAHEVPAGEERIRHLKL
                     DRFLSAAALDQYFQRPSDFELEQHLGQGIGIFSGGKVRDFRIRISARGARWVLEDPWH
                     PHQKAEPQKGGGIILTVPAHHDLEIIPRVLALGSEAELLAPASCRRAITAIVKTLAER
                     YKEG"
BASE COUNT          873 a         1469 c         1594 g          812 t
ORIGIN      
        1 ctcagctcaa taaagccgcg aaggcgattc aggcgttgcc gcggcaataa tcccttcgcg
       61 gggcgccagg tcacgagcga gtgcctggct ggcagtgtgg gacggatctc gctgcaaagc
      121 cgcgagtgcc gactggcggg cggcagaagt atcgcccgcg cgaagctgga cgcgggccag
      181 ctcgtaatag agttctgccg aggcattgcc gcgctcagcc gcctgagcca acgcgatggc
      241 cgcgtcctga aagcggccga gatcgcggag ggcaaagccc tcgcggatca gcacatcggc
      301 agggacctgc gcgggaggat agttcgccgc cagggcctgt agcgttccca gcgctcgctg
      361 cggtcggccg tcttgcgcgt aaatgtccgc aatcgcgagc tgcacctcgg gatactgctc
      421 gcgaagactg agtgcccggt gaaatttcgc gagcgcctct tcgcggcggc cttcggcctg
      481 ctcgacctgc gcccgcaggg cccaggccgc cgcaagctgc gaattggccg cgatggcccg
      541 atcggcctgc tggccggcgc gctgcaattc gcctcggaac agatacattt cgccgagctg
      601 caccagtcgc tccggatcgt ggcccgagag ccgcaccgcc tcttccatgt gggagacggc
      661 ttgatcgcga gctccgcgct gccacagcgc ctgagcgtag ccgcaccgcg cccgctcgtc
      721 ggcaggacac tgctgcacgg ctgcggcgaa gagcgcctcg gattgctccc agcggccgtg
      781 ctcttggagc tccatcgcgt ggagcgaaag ctgccgggcg gaagccagcg cctcgtcctg
      841 ggcctgctcg caccgcagcg cgcggcagcc gccgacagaa gcgagccaag cgccagccat
      901 cagcacgatc gccagtgacc ggcctgtccg cacgaagctg cctgttgcga ccagcgcacg
      961 gcggcctcga tgagcatggc gatggtttgg catccgtgcc cggcttggag aatgtccggc
     1021 aactcgacaa cggtccctaa tcgcaactta tcgacatttt gacgccagca tccgcactca
     1081 atccgcagga gcttccgcga cgcttgatcg ctggcggacg tgtcgggatt ggtccaattg
     1141 ggggaagtac caaactcagg ccgcacgggc aaggagaaac gcgtcgcgcc cagctagcac
     1201 cggacgtctg gtggtcgcag tctcgggtcg cggataggct gaatcgctgg ttctgcccaa
     1261 cgagactgtc catgcccgcc cttttcgaac gctttggtgt caagattctc taccctgaga
     1321 actggtcgct catggacctg gaggttgaag actggccccg ctcggtcacg atccagagcc
     1381 aggacacggg cttttggtcg ctgcacgttt atccgccgca acaggaggcc gagccgatcc
     1441 tggaagagct ggtcagcgcc atcggcgaag gattcagcga tgtggaggtg ctgccggcca
     1501 ccgagcagtt cggcgagacg agcaccgccg gggtcgacct cgcctttttc tatctcgacc
     1561 tcctcgtcga agccaaaatt cgctacgtcc acacgcccag cgccatgctc gtctggcact
     1621 accaggccga aagccgcgag tttgagagaa tcgaaccggt tttccaggcc atcgccacca
     1681 gcttgctgcg gacgcaagtg gcgattaagg aataaaatgg tccggtagga aagcgcccgt
     1741 tcttgggtcg ccacaaatca agggaacccg accgttctta aacacgcagc gaatgataag
     1801 ctgcggcctg ggcggggagc cgcaaagtgg tgatgggggc gaagaaaatg cttgacagaa
     1861 ctggggggcg gttcaatgca tagcgagctt gggtgcttgt caggcggcga atgcaagagc
     1921 tgttcgagat ttaaggggaa attgagcgtc ggcgtgcccg ctagcagagc ttcggtcaat
     1981 gcgccgtgcg tgccgtggag gacggcgaga tcgcaggatt gcagcacatg gcgcaaatcg
     2041 gcaggtcctc tcagaaccaa cgccccgctg ccggtcatgg ccttgacgcg ctcgtgcggg
     2101 agttcaggaa catagacgat tgaggcgacg cccagttccg gaatcgcctt caagagcggt
     2161 tccagcgccg ggcagggctt caggtagaag aaaacacgtc gcgagcctga cggccagtcg
     2221 ggccggacgc cccaggagct gttccacgtt ccccaatatc gcatgccggg ctgcgcgcca
     2281 tttggatcga gttcggggaa cgtgatcagc tctgtggcgt cagcgtcggc atatagcggg
     2341 ccgattcgcg gcaggggcgg ttgtccgagg cgggccaaga cggtgttgat tcgcgccaga
     2401 acgctggctt cgatgtctcg cggtggcgtc gcgcccagcc aagggcgcaa atcctgcagc
     2461 gggtcggagt ccgcgggaca cagccgtctt ggcgctggtc cctcgcacgc tcagtgcggc
     2521 tatgggacta tgctccagaa tcaccagttt cggctggcgc atgcccagca gattccacca
     2581 agccatggcc agcgaggtca attcctccac gtcgccgaat ccgacgttgt gcaggatgtc
     2641 ggcgaaagtt cgcggctcgg tgatttcgtt ttgcgggcgg ccgatcttcg cgggcgcttg
     2701 aaaccagcga atcggcgtcc ctaggaacac cgtgctgcag aaccgcacat cgcggacggc
     2761 gaacgcgacc gacctgccgc ggcgtacgaa cgcttccgcc aagggtcgca gccgcccaaa
     2821 tatgccccaa tccccccgcc aagttcccaa gtaaggagca catcgaacat cggccgcgct
     2881 tgctcctcac ttggttgtcg ccgcataccg gattggatcc gtcgcgccgt tttccgcgaa
     2941 acccttcagc cgcagcaggc acgagtcgca gtggccgcag gcgaggccag cgggatccgg
     3001 gtcgtagcag cttaaggtga gggagtaatc gacgccgagg tccaagccgc ggcggatgat
     3061 ctgggctttg gtcagctgaa tcagcggcgt gtggattttg acccgttgtg tcccctccac
     3121 ggcggctttg gtcgcgagat tggccatccg ctcgaaagct tcgatatact ccggccggca
     3181 gtcgggatag ccgctgtaat cgagggcgtt gacgccgagg aaaatgtcgc tgctgccggt
     3241 cacttcggcg aaggcgatgg cgaacgagag aaagatggtg ttgcgggccg ggacgtaggt
     3301 gatcgggata tccgagccga tctcgtcggc gctctccttc ttcggcacgg ccagatcgct
     3361 ggtcagcgcc gacccgccga actggcggag gtcgatctcg acgatccggt gcgatgccgt
     3421 gccctggctc ttcgccaccc gctcagccgc cgccaattcc accgcgtgcc gctggccgta
     3481 gcggaagctc agcgcgtgcg gctcgtagcc tgcgcttttg gcgatggcca ggaccgtagc
     3541 cgaatcgagc ccgccgctga ggaggacgac ggcgctgggc ttggaagcgg acattgtttg
     3601 ctatgatggc acggcccatc tatcgagcaa agccccatgt cgcgcaacga gcagctcatc
     3661 cgccagcaca agctgttgca gcttctggag aacacccgca tcggccgcac gctggaggag
     3721 ctgcgtgacg atctggtcga tcagctcggc ctctcggccc tgcacatcaa gagcgtgaag
     3781 cgcgacctgg aggccctcga agctgccggc ttccgcatcg aagcccagca cctgcagcgc
     3841 ggcaaggtct ggcgactggc cccgcacagc aagagcgcgg tcaaggtgca gccctcggcg
     3901 accgaactga tcgcgctgtc gatcgggcgg gacctgctgt atccgctggc ggggacgccg
     3961 ttctggcagg ggatcgagac gttctggaac aagctgaaag aggagatgcc ttccggggta
     4021 tggaagcact acgaggcgta ccggcaggtg ctctatgtcc gcggcatgcc caccaaaagc
     4081 tatgcccggc agcatgggat gctgggcacg attcaccggg cgattgtcga gcggcggctg
     4141 ctcagcgtgg aatatgagcc gcccggcaag gagccgcagc ggcggctgat cgagccgtac
     4201 gcggtcgtgt tctaccagtc gagcctgtac atcgtcgcgg cagctcacga ggtgccggcg
     4261 ggcgaggagc gcatccggca tctcaagctc gaccggttcc tgagcgccgc ggcgctggac
     4321 cagtatttcc agcggccgag cgacttcgag ctggagcagc acttggggca agggatcggc
     4381 atcttcagcg gcggcaaagt ccgcgatttc cgcatccgca tctcggcccg cggtgcgcgg
     4441 tgggtgctgg aagacccgtg gcatccgcat cagaaggcgg agccgcagaa aggaggcggg
     4501 atcatcctca cggtcccggc ccatcacgat ctggagatca tcccgcgtgt gctggccttg
     4561 gggagcgaag ccgaactgct cgcgccggct tcgtgccggc gggcgatcac ggcgatcgtg
     4621 aagacgctgg cggagcggta caaggagggt tagagattag gggttagaaa tgagggaacg
     4681 tgacacgggc cagtggcttt ggccggtagc gggatcgggc aggattcgct gtgaaggcat
     4741 cagttggc
//
LOCUS       DASUGB010001023         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_391229, whole genome shotgun sequence.
ACCESSION   DASUGB010001023 DASUGB010000000
VERSION     DASUGB010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391229"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1772
                     /locus_tag="VFV87_19405"
     CDS             57..1772
                     /locus_tag="VFV87_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5105999.1"
                     /translation="MATRASLGGIIHTYQKYDPVNLPAPTQPPPDLVSPALEHFLMFG
                     DARRLSDAELARAVRLDPSQIAGMGPSLDALLAMLLERKRKILAKYETSSVQGLARKE
                     YQKAGSKIEPPPKLRSRFQRALAQEQLRDLEMLWYSVDDERSKFARQLVQLVDTLGNK
                     YQVDELAAKYEFTGRTPLTIPEALAVKEELEKIDQLLKQLEEARENAQIGLIDLELLE
                     QFTEPGDMAKLEELQRMVENYVREMAERQGLERDGKGFRLTPKAYRIFQGRLLEKIFS
                     NLQESRSGRHQGPIVGEGAVETTATKGYEFGDSLAHMDIPQSLINALLRRSSEGDGND
                     GPLRLKQDDIVIHKTRNTPKCATVVIMDMSGSRRYDGQYVNVKRMALALEGLIRREYP
                     GDYLQLLEMYTFCRPTPPGKIIDLMPKPVTIFDPYVRMKIDMSRDDVSEYAIPQHFTN
                     IQRSLQMARQFLAAQDTPNRQVILITDGLPTAHFQGSWLYMLYPADPLTENATLREGL
                     LAAREGITINIFLVPSWSQSEEDIRFAYRLAESTKGRVFFTAGRDLDRYVVWDYVKRK
                     REIVA"
     gene            1889..2806
                     /locus_tag="VFV87_19410"
     CDS             1889..2806
                     /locus_tag="VFV87_19410"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HEX5106000.1"
                     /translation="MACTAAADEAAFQAEKHDDRFVITHGGKPVATYVFRDEKILRPY
                     FAHVHAPDGTQVTRHHPPRPGIDAVDHDTMHPGIWLAFGDVSGADFWRNKGQVDVEHI
                     NESPRLGDNFVSIVGYNHYAAGGQEIGRELFTRAIRIVGDGYLLSFYSRFDAPKSRPF
                     TFGDQEEMGLGLRLATPLAVKGGSGTITNSTGGKNEKEVWGKQADWCDYSGIIEREGE
                     KRRVGMLLIPHKDNFRRSWFHARDYGFVAANPFGQNAFTNGEKSRIEVKPGEHLDLRF
                     GVWIYSTPADQPPDLDSIAWEYHRLAYAR"
BASE COUNT          597 a          861 c          925 g          501 t
ORIGIN      
        1 cgggcggcgg cgtggatgtc caatcccgca cctgcccaat cccctacaat gcggacatgg
       61 ccacacgtgc aagtctcggc gggatcattc acacgtacca gaagtacgac ccggtcaacc
      121 tgccggcgcc gacgcagccg ccgccggatt tggtgtcgcc ggcgctggag cattttttga
      181 tgttcggcga cgcccggcgg ctgagtgatg cggagctggc gcgggccgtg cggctcgatc
      241 cgagccagat cgccggcatg gggccgagcc tggacgccct gctggccatg ctgctggagc
      301 ggaagcggaa aatcctggcg aaatacgaga ccagcagcgt gcaggggctg gcccgcaagg
      361 aataccagaa agccggcagc aagatcgaac cgccgccgaa gctgcgaagc cgcttccagc
      421 gggccctggc ccaggagcag ctccgcgacc tggagatgct ctggtattcg gtcgatgatg
      481 agcggtcgaa atttgcccgg cagctcgtgc agcttgtcga tacgctgggc aacaagtacc
      541 aggtcgatga actcgccgcg aaatacgagt tcaccggccg cacgccgctc acgattcctg
      601 aagcgctcgc ggtgaaggag gaactggaga agatcgacca gcttctcaag cagctcgaag
      661 aagcccgcga gaatgcccaa atcggcctga tcgacctgga gctgctggag cagttcaccg
      721 agccgggcga tatggcgaag ctcgaagagc tgcagcggat ggtcgagaac tacgtccgcg
      781 aaatggccga gcggcagggg ctggagcgcg acggcaaggg ctttcgcctg acgcccaagg
      841 cctaccgcat cttccagggg cggttgctgg agaagatttt cagcaacctg caggaatcgc
      901 ggagcggccg gcatcagggg ccgatcgtcg gcgaaggagc agtcgagacg acggcgacca
      961 agggctatga attcggcgat tcgctggccc acatggacat tccgcagtcg ctgatcaatg
     1021 ccttgctccg tcgcagctcc gagggcgatg gcaatgacgg gccgctgcgc ctcaagcagg
     1081 acgacatcgt cattcacaaa acgcggaaca cgcccaagtg tgcgacagtc gtgatcatgg
     1141 acatgagcgg ctcgaggcgc tacgacgggc agtacgtgaa cgtcaagcgg atggcgctgg
     1201 ctttggaagg gctgatccgc cgcgaatacc cgggcgacta cctgcagctc ctggagatgt
     1261 acaccttctg ccggccgacg ccgccgggga agattatcga cctgatgccc aagccggtga
     1321 cgatcttcga tccgtacgtg cggatgaaga ttgacatgag ccgtgacgac gtgagcgagt
     1381 atgcgatccc gcagcacttc acgaacatcc agcggtcgct gcagatggcc cggcagttcc
     1441 tggcggcgca ggatacgccg aaccggcaag tgattttgat cactgacggc ctgccgacgg
     1501 cccatttcca gggctcgtgg ctgtatatgc tctatccagc cgatccgctg accgaaaacg
     1561 cgacgctccg cgaggggctt ttggcggccc gcgaggggat cacgatcaac atttttctcg
     1621 tgccgagctg gtcgcagtcg gaggaagaca tccgcttcgc ctatcgcctg gcggagagca
     1681 ccaaggggcg cgtcttcttc accgccggcc gcgacctcga ccgttacgtc gtctgggact
     1741 acgtgaagcg caagcgggag atcgtggcat gacagtaacc cgaagcgtca gcgagggagt
     1801 cagcgatatg tttcgcggcc atgccatctt gttcttcata gcactccctc gcttacgctt
     1861 cgggttggtg tgcatcgtcg ctttcgcgat ggcatgcacc gccgcagcgg acgaagccgc
     1921 cttccaagcc gagaaacacg acgaccggtt tgtcattacg catggcggga agcccgtcgc
     1981 aacctacgtc ttccgcgacg agaagatcct gcgaccgtat ttcgcccacg tccacgctcc
     2041 ggacggcacg caggtgacgc ggcatcatcc gccgcggccc ggcatcgacg cagtcgatca
     2101 cgatacgatg catcccggaa tctggctggc gttcggcgat gtgagcggtg cagatttctg
     2161 gcgcaacaag ggccaggtcg acgtcgagca tattaacgaa tcgccaaggc tcggcgacaa
     2221 ttttgtttcc atcgttggct acaaccatta cgctgcgggt ggccaggaaa tcggtcgcga
     2281 attgttcaca cgtgcaatac gcattgtcgg cgacggttat ttgctgagtt tctacagccg
     2341 gtttgatgcg cccaagtcaa gacccttcac cttcggcgac caggaagaaa tgggcctggg
     2401 cctgcggctg gcgacgccgc tggcggtcaa aggcggcagc gggacgatca ccaatagcac
     2461 cggcggaaag aacgagaaag aagtgtgggg aaagcaagcc gactggtgcg actacagtgg
     2521 gattatcgaa cgagaaggag agaagcggcg cgtcggcatg ctactcattc cgcacaagga
     2581 caacttccgc cgctcctggt tccacgcccg cgattacggc tttgtggcgg ccaatccctt
     2641 cgggcagaac gccttcacca acggcgaaaa gagccgcatc gaagtcaagc caggcgagca
     2701 cctggacttg cgtttcggcg tttggattta ttccactccc gccgaccagc cgccggacct
     2761 ggacagcatt gcatgggagt atcaccgatt ggcttatgcg cgctagtcct caggtggttt
     2821 gggagtgtgg agcacgacga cgaagcactc gtgctgcacc cagtggggcg tcaggtagaa
     2881 gcgg
//
LOCUS       DASUGB010001024         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_531998, whole genome shotgun sequence.
ACCESSION   DASUGB010001024 DASUGB010000000
VERSION     DASUGB010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531998"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2611
                     /locus_tag="VFV87_19415"
     CDS             <1..2611
                     /locus_tag="VFV87_19415"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5106001.1"
                     /translation="DIFITELDARPLFDGGSLAGWEGAGEPAEKCWRAEAGSILCTGE
                     KGPWLRSMEQFGDFNLRLEYKLKAGGNSGVYIRVPESGNHHGNGAGVEIQVLDDKAER
                     YKNLKPYQFTGSVYAVVPASPHVSREAGQWNSLEINCRGSAYHIIHNGVTIVAADEAT
                     WPGLKGRLASGFLGLQNHSEEVWFRNLRISPAFELPVPPPAIPDVAQLKEILSKPILD
                     PALPQQEVEAFCDARVRRLPAEGAPQRASPEAWLKYAEQVRQDVLNNVVFRGEAAKWR
                     DADCKVEWLETIDGGEGYKIKKLRYEALPGLWIPALLYEPTKLEGKAPVFLNVNGHDG
                     AGKAAPYKQIRCINQAKRGIIALNVEWLGMGQLRGPNFAHYKMNQLDLCGTSGLAPFY
                     LAMSRGLDLLLKHPHADPSRVGVAGLSGGGWQTITISSLDTRVTLCNPVAGYSSFITR
                     VANHSDLGDSEQTPCDLALHADYAHLTTLLAPRVALLTYNEKDNCCFAAPHALPPLLE
                     AARPVYKLLGAADSLHDHINYDPGNHNFERDNREALYKVVGAHWYSGDGTFSPNEIDS
                     AAEVKTAEQLAVELPADNFDFQKLAEHLMQDLPHAASPPADAASAAKWQAAEREKLAA
                     LLRMGKYAPQPLEYKITPEEVSREAVGMLEVVRWKLKIGEVWTIPAVELALGSPAETT
                     ILLADGGRAEAGEQVPALLSSGHRVLAVDPHYFGESKIKSRDFLFALFVSSVGERPLG
                     VQASQIGGIARWLGVDRKMGPVTITADGPRMSLVALASAALEDKPIASVNLHGSLGSL
                     KEVIEQGGAVNTTPEQFCFGLLEQTDISRLTMLVAPREVRFASPSERVQKELVTARDW
                     YHLIGKDFDPLAN"
     gene            2618..>3200
                     /locus_tag="VFV87_19420"
     CDS             2618..>3200
                     /locus_tag="VFV87_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106002.1"
                     /translation="MTRCCCFASLAVLVFTSPPANTADWPQFRGPAGDGRATAKNLPT
                     EWNETTNVAWKTEIPGRGWSSPSLYQNRLHLTTAVPLAAESGNGPLSLRAICVDASSG
                     RILWNEPVFEEPADAPKIHSKNSHASPTPLVEDGRVYVHFGHQGTACLDLAGKVLWKN
                     DRLKYPPVHGNGGSPVLEGGLLIFSCDGGSDPFV"
BASE COUNT          611 a         1039 c          975 g          575 t
ORIGIN      
        1 ggatattttc atcacggagc tcgatgccag accgctgttc gatggcgggt cgctcgcggg
       61 ctgggaaggg gcgggcgagc cagccgagaa atgctggaga gcggaggccg gctcgatcct
      121 ctgcaccggc gagaaaggcc cctggctgcg ttcgatggag cagttcggcg acttcaatct
      181 gcggctggag tacaagctca aagcgggtgg caatagcggc gtgtatatcc gcgttccgga
      241 gagcggcaac catcacggca acggcgccgg cgtcgaaatc caggtgctcg acgacaaggc
      301 cgagcgctac aagaacctca agccgtatca gttcaccggc agcgtctatg ctgtcgttcc
      361 cgcctcgccg cacgtcagcc gcgaagcggg ccagtggaat tcgctcgaaa tcaactgccg
      421 cggctccgcg taccacatca tccacaacgg cgtcacgatc gtcgcggccg acgaagcgac
      481 ctggcccggc ctcaagggac gcctggccag cggctttctg ggcctgcaga accacagcga
      541 ggaagtctgg ttccgcaacc tgcgaatcag ccctgctttc gagcttcctg ttccgccgcc
      601 cgcgattccc gatgtcgctc agctcaagga aattctcagt aagccgattc tcgatcccgc
      661 actgccgcag caggaagtgg aagcgttctg cgacgcccga gtccgccgct tgccggctga
      721 aggcgcgccc cagcgggctt cgcccgaggc ctggctcaag tatgccgagc aagtccggca
      781 ggacgtgctg aacaatgtcg tcttccgcgg cgaagccgcg aagtggcgcg atgccgattg
      841 caaggtcgag tggctggaga cgatcgacgg cggcgaaggc tacaagatca aaaagctacg
      901 ctacgaggcg ctccctgggc tgtggattcc cgccttgctc tacgagccga cgaagttgga
      961 gggcaaagcc cccgtctttc tgaacgtcaa cggccacgac ggggccggca aagccgcgcc
     1021 gtacaagcag atccgctgca tcaaccaggc caagcgcggc atcattgcgc tgaatgtcga
     1081 gtggctcggc atggggcagc ttcgcggccc gaatttcgcc cactacaaga tgaaccaact
     1141 cgacctctgc ggcaccagcg gcctggcgcc gttctatctc gcgatgagcc gcgggctcga
     1201 cctgctgctg aagcatccgc atgccgaccc atcacgcgtc ggcgtcgctg gcctttccgg
     1261 cggcggctgg cagacgatta cgatcagctc gctcgacacg cgcgtgacgc tgtgcaaccc
     1321 ggtcgccggc tattcaagct ttatcactcg cgtggcgaat cactccgatc tgggcgattc
     1381 cgagcaaaca ccttgcgatc tggcactgca cgccgattat gcgcacctga cgacgctgct
     1441 ggccccgcgg gtcgccctgc tcacctacaa cgagaaggac aactgctgct tcgccgcgcc
     1501 gcacgccctg ccgccgcttc tggaggcggc ccgtccggtc tataagttgc tcggtgcggc
     1561 ggattcgctg catgaccaca tcaactacga ccccggcaat cataatttcg agcgtgacaa
     1621 ccgcgaggcg ctctataagg tcgtcggcgc ccactggtat agcggcgacg gaacattttc
     1681 gcccaacgaa atcgactccg cggctgaagt aaagactgcc gagcaacttg ctgtcgaatt
     1741 gcccgcggat aatttcgact ttcaaaaact ggccgaacac ctgatgcagg acttgcccca
     1801 cgccgcaagc ccgccggccg atgctgcttc ggccgccaag tggcaagccg ccgagcgtga
     1861 aaagctcgca gcgctgctgc gcatgggaaa gtacgcgcct cagccgctgg agtacaagat
     1921 tacgcccgag gaggtttccc gcgaggcagt cggaatgctg gaagtagtac gttggaagct
     1981 caaaatcggt gaagtgtgga cgatcccagc ggtggagctg gcgctcggct ctccagcaga
     2041 gacgaccatc ctgttggctg acggcggccg cgccgaggcc ggcgagcagg tgcctgcgct
     2101 gctctccagc ggccaccgcg ttctcgccgt cgatccccac tacttcggtg aatccaaaat
     2161 caagagccgc gactttctct ttgcgctctt cgtctcttcc gtgggcgaac gacccttggg
     2221 tgtgcaagcc agccaaatcg gcggtatcgc tcgctggcta ggtgtagatc ggaagatggg
     2281 ccccgtcacg ataactgccg atggaccgcg catgagcttg gtcgcgctgg cttctgcggc
     2341 gctggaagac aagccgatcg cctcggtcaa cctgcacggc tcgctgggct ctctgaagga
     2401 ggtcatcgag caaggcggtg ctgtgaatac cacgcccgag cagttctgct tcggcctgtt
     2461 agagcagact gatatttccc ggctcacgat gctcgtcgcc ccgcgcgagg tgcgttttgc
     2521 ctcgccgtct gagcgagtcc agaaggaact cgtaacggcc agggactggt accacctgat
     2581 cggcaaggat ttcgatccgc tcgccaacta ggaaattatg actcgctgct gctgcttcgc
     2641 gtccttggcg gtcttggtgt tcacctcccc gccggccaat accgccgatt ggccgcagtt
     2701 ccgcggtcct gccggagacg gacgcgccac cgccaagaat ctgccgacgg aatggaatga
     2761 aacgaccaac gtcgcctgga aaaccgaaat tcccggccgc ggctggtcgt cgccgagcct
     2821 ctatcagaac cgcctgcatc tgacgaccgc cgtccccctc gctgccgaaa gtgggaatgg
     2881 ccccctctcg ttgcgggcaa tttgcgtcga tgcctcctcg ggccgaatcc tctggaacga
     2941 acccgtcttc gaggagccag ccgacgcccc aaagatccat tcgaaaaaca gccacgccag
     3001 tcccacgccc ttggtggagg atggccgcgt ctacgttcac ttcgggcatc agggcaccgc
     3061 ctgccttgat ttagccggca aagtgctgtg gaagaacgac aggctgaaat acccgccggt
     3121 ccatggcaat ggcggctcgc ccgtgctgga aggcggtctg ttgatcttta gctgcgacgg
     3181 cggcagcgat ccgttcgtcg
//
LOCUS       DASUGB010001025         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1781693, whole genome shotgun sequence.
ACCESSION   DASUGB010001025 DASUGB010000000
VERSION     DASUGB010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781693"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFV87_19425"
     CDS             <1..497
                     /locus_tag="VFV87_19425"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX5106003.1"
                     /translation="DPASPKFAPRSKRSPNEGGIRTPIMLRWPGKLKPQQDEKTLVST
                     IDLAPTILAACGLPTTKEMTGISLLDSGKEAAERNAVFGEIYEHDVADIDDPEPGLLF
                     RWCVAGDWKLIESADGKSRGLYNVRSDPQEVKDLASEQQQRVRELGGRIKSEWSKHQG
                     PSRN"
     gene            complement(454..1116)
                     /locus_tag="VFV87_19430"
     CDS             complement(454..1116)
                     /locus_tag="VFV87_19430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5106004.1"
                     /translation="MVLVHGLAANRTIMAPLGRALRRHFPQVTNWGYQSLWYPIERHG
                     RELARRLRDIDRNAKGRVHLVTHSMGGIIGRLALADYVPTNFGRFLMIAPPNRGSRVA
                     RWLAPLLGRIFPPLVQLTDASDSFVCSLPRPAIAELGVIAARGDFLVCEPSTRLGCET
                     DHIILPGLHSSLLWSKATARQTSHFLKHGRFWRADGASVSQNCQEAISCEMGPGVSTT
                     PT"
     gene            1391..>2356
                     /gene="argH"
                     /locus_tag="VFV87_19435"
     CDS             1391..>2356
                     /gene="argH"
                     /locus_tag="VFV87_19435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037227775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5106005.1"
                     /translation="MATPSRSGVFSQAADRRVERFTESISFDHRLYAHDIQGSVAHAL
                     MLAHVGLISAHECNQICETLDQIKSEIEAGNFPLRTELEDIHMHIEQALIDRLGDVGR
                     KLHTGRSRNDQVATDLRLWTRDSLDRIDRGLLELQKAFVGRCDGDFDVTLPGYTHLQR
                     AQPVLAPHYWLAYCEKLQRDRERVADCRRRTNVCSLGAAALAGSSLPIDRHEVAKRLH
                     FEGVAANSLDVSSDRDFVLESAFVLALIAEHLSTWAEEWILWSTTEFGFLKLPHEFCT
                     GSSIMPQKVNPDVLELTRGKTARVIGNLQALLVLLKGLPLAYNRDL"
BASE COUNT          446 a          758 c          735 g          417 t
ORIGIN      
        1 aggaccccgc gtccccgaag ttcgccccgc gcagcaagcg ctcgcccaac gaagggggta
       61 tccgcacgcc gatcatgctc cgctggccag gcaagctgaa gccacagcag gacgagaaaa
      121 cactcgtcag cacgatcgac ctcgctccga ccattctggc cgcctgcggc cttccgacga
      181 cgaaggagat gaccggcatc agcctgctcg attccggcaa ggaggcggcg gagcgcaatg
      241 ccgtcttcgg cgaaatctac gagcatgacg tggccgatat cgacgatccc gagcccggcc
      301 tactcttccg ctggtgcgtt gccggcgact ggaagctgat cgaatcggcc gacgggaaat
      361 cgcgcgggct ttacaacgtc aggagcgatc cgcaggaagt gaaagatctt gcgtctgaac
      421 aacagcagcg cgtgcgcgag ctaggcgggc gaatcaagtc ggagtggtcg aaacaccagg
      481 gcccatctcg caactgattg cctcctggca gttctgagag accgaagcac catcagcccg
      541 ccaaaatcgg ccgtgcttca gaaagtggct cgtctgccgt gcagtcgcct tgctccagag
      601 gagcgaggag tgcagcccgg gaagtatgat gtggtccgtc tcgcagccca gacgggtgct
      661 cggctcgcag accagaaagt cgccgcgggc ggcgatcacg cccaattccg cgatggctgg
      721 ccgcggcagc gagcagacaa aactgtcgct tgcatcggtc agttggacca gtggcggaaa
      781 gatgcgtccc aaaagcggcg ccagccaccg cgcaacgcgc gagccgcggt tcggaggcgc
      841 aatcatcagg aagcgcccga agttcgtggg tacgtaatcg gccagcgcaa ggcggccaat
      901 gattccgccc atgctgtgcg tgacgaggtg aacccgcccc ttggcgtttc ggtcaatatc
      961 ccgcaggcgc ctggccaact ccctgccatg ccgttcgatt gggtaccaca agctctggta
     1021 gccccagttg gtgacctgcg ggaagtgcct gcggagcgcc cgccccagcg gcgccataat
     1081 ggttcgattg gctgccaggc cgtgaaccag caccaccgtg tcactcgtcg agatagattc
     1141 tgtccgctcc atggctgatt cgtcgcaaag ctcccttatg tattgtagcg ctggtgcaac
     1201 gatcgtgccg gaaattgggc gatcgctcgt ttcgctgcgt tgacgcttcg ccgtaaccca
     1261 tttagcataa aagagttgct gcgagctgca gggtgcatga gcgaagcgca atgcaccatc
     1321 gcacgtgtcg attagagccg gtgcatttcg gccgccgcgg cctcatgcac cctacctatg
     1381 gggtttgctg ttggccacgc cgtcacgcag tggagtcttc tcgcaggcgg cggatcgccg
     1441 cgtggagcga tttaccgaga gcatcagctt cgaccaccgc ctctatgccc acgatattca
     1501 gggctcggtc gcccatgcct tgatgctggc ccacgtcggg ctgatctcgg ctcacgagtg
     1561 caatcagatc tgcgagacac tcgatcagat caagtccgag atcgaggccg gcaacttccc
     1621 gctgcgcacg gagctggaag acatccacat gcacatcgag caggccctga tcgaccgcct
     1681 gggcgatgtc ggccgcaagc tccacaccgg ccgcagccgc aatgaccagg tcgcgaccga
     1741 cctgcggcta tggacgcggg attctttgga ccggatcgac cgcgggctgc tggagctgca
     1801 aaaggcgttt gtcggccgct gcgacggcga ttttgacgta actttgcccg gctatacgca
     1861 cctgcagcga gctcagccgg tgctcgcgcc gcactactgg ctggcctatt gcgagaagct
     1921 ccagcgcgac cgcgagcgcg tggccgattg ccgccggcgc acgaacgtct gctcgctcgg
     1981 cgccgccgct ctcgccggca gcagcctgcc gatcgaccgg cacgaggtgg cgaagcggct
     2041 gcatttcgag ggcgtcgcgg ccaacagcct agacgtctcg agcgaccgcg atttcgtcct
     2101 ggaatcggcc ttcgtcctgg ccctgatcgc cgagcacctc agcacctggg ccgaggagtg
     2161 gattctttgg tccaccaccg aattcggctt cctcaagctg ccgcacgagt tttgtacagg
     2221 cagctcgatc atgccgcaga aggtcaatcc ggacgtgctg gagctgaccc gcggtaaaac
     2281 ggcccgcgtc atcgggaatt tgcaggcttt gctggtcctc ctgaaggggc tgccgctggc
     2341 ctacaaccgc gatttg
//
LOCUS       DASUGB010001026         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1781727, whole genome shotgun sequence.
ACCESSION   DASUGB010001026 DASUGB010000000
VERSION     DASUGB010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781727"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..2045
                     /locus_tag="VFV87_19440"
     CDS             120..2045
                     /locus_tag="VFV87_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106006.1"
                     /translation="MITWEEAIAHPRFWLSAGTLLSVAAALCLLINWAAEGQRVRLAR
                     VLLAWTAIMALVPIRAYVPALVFAWVAAMTFLLVRMIQFLSRERAAGESRWTGWQRYS
                     LTDLMVLTLFAGTLIGLAMRLPDSPLALLGSAIVASCLAFLASLSYQVALSKRRAFLL
                     VLLAATLVALAAPGRILWSLPLSRDFADNKYEGYKLFLHEVFLSLLILLSYFAFILSR
                     ATWKEWPVPRRLVRIGWIGFIASTTLFGLFIWYLQGAMSWQLEDEWRRIVLTLASTMP
                     LLALAAIIMGIAVLAHWYVKLSPGTARRAATTSLSVGALAVALPLGWLYWQMFYLTPL
                     VPFAPQSPTNYERLTEIADELRVETDIGRANLDSSRTKILVTEASELLKVPNHIPLSA
                     VRRQIDEKVAVTDLRQTIAIEEAFAASAAELAEAGQHGRAADRLLDLMRFGKMLQRGG
                     TSTHSAQGEHVQERVLKDLRDLSPQLSPSESLATIRALGKAILMPEDAQALDAREHAV
                     NERVYDWHTHWVGTVYRVFPPERRLVPASRDTANLMMAGELAIRGAQLGLAHDQFCNV
                     TGRPAVAISELVPEYLPALPLDPFTRQPLRTCRTADEDLVIYSVGPDGIDNGGNFARY
                     AHQPYEKPGYDIRVTFD"
     gene            2078..2623
                     /locus_tag="VFV87_19445"
     CDS             2078..2623
                     /locus_tag="VFV87_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106007.1"
                     /translation="MVSGTEQALCLFGSSLVILPNMHPKLNRQSMLLGTAVVVVLLLA
                     FVALLVGIDRRSPLSPIEARLIGEWSHDPAETVRSFRADRSFSTSNGQFVGTWDVRDG
                     RLTVTYWQPFELPTDFSAAALTHSIRRTRRETLSWRIEFSADNQQLSLDQGCVLQDGR
                     LIPIRAGNEKWLFTRVRNAAK"
     gene            2700..3257
                     /locus_tag="VFV87_19450"
     CDS             2700..3257
                     /locus_tag="VFV87_19450"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5106008.1"
                     /translation="MRPLRYSINVTLDGCCDHRAGSTDEELHRFWAENLAQADALLFG
                     RVTYEMMEAAWRPSATGVKPDWMAEWMEPFARTIDAAKKYVVSSTLDRVDWNAELVRG
                     DLGQAVEQLKQQAGNGLFTGGVKLPLALTELGLIDEYEFVVHPRLAGHGPTLFAGLSK
                     YVDLKLVDRLEFGSGAVAMRYEPRR"
BASE COUNT          625 a         1011 c         1050 g          689 t
ORIGIN      
        1 gcttcgaaag cccccccgca cgttcgccca aaaagccaag ccatcgcagc cgcgctggac
       61 aatcgcacgg gcggcgcggt tgaattggaa cgtaatttac tgacttgctg taaacatcca
      121 tgattacttg ggaagaggcc atcgcccatc cccgcttctg gctgtcggcc gggacgctcc
      181 ttagcgtggc cgcggcgctg tgcctgctca tcaactgggc ggccgagggg caacgcgttc
      241 ggttggcgcg cgtgctattg gcgtggactg ccatcatggc gctagttccc attcgggcgt
      301 acgttccggc gctcgtgttc gcgtgggtcg ccgcgatgac attccttctt gtgcgaatga
      361 tccagttcct ctcgcgcgag cgagccgcag gagagtcgcg ctggacaggt tggcagcgct
      421 attcgctgac ggatttgatg gtgctcacgc tgttcgccgg cacgctgatc gggctggcca
      481 tgcgtctgcc ggactcaccc cttgcgctcc ttggctcggc gatcgttgca tcgtgcctgg
      541 ccttcctcgc cagcctcagc tatcaagtgg cgctaagcaa gcgtcgggcg ttcctgctcg
      601 ttcttttagc ggcgacgctc gttgcgctcg ccgccccagg aagaatcctc tggagtctgc
      661 cgctttcgcg cgactttgcc gacaacaagt acgaaggcta caaactcttc ctgcacgagg
      721 tattcctgag cctgctcatt ctcctgagct atttcgcctt catacttagc cgggcaacgt
      781 ggaaagagtg gcctgttccc aggcgcttgg tccgcattgg ctggattggg ttcattgctt
      841 ccacaacgct gttcggcctc ttcatttggt atctccaggg cgcgatgtcc tggcaacttg
      901 aggacgagtg gcgacgcatc gtgctgacgc tggccagcac catgcccttg cttgcgctcg
      961 ccgccatcat catggggatt gccgttctgg cgcactggta cgtaaagctc tcgccaggca
     1021 ctgcccggcg ggccgcgacg acgtccctga gcgtcggggc actggctgtg gcgttgccgc
     1081 tgggttggct gtactggcag atgttctacc tgacgcctct tgttcctttc gcgccacagt
     1141 cgccgacaaa ctatgagcga ttgacggaga tcgccgacga gttgagggtc gagaccgaca
     1201 tcgggcgggc caatctggat tccagcagaa ccaaaatcct ggtgacggaa gcatccgaac
     1261 tgctaaaagt gccaaaccac attccgctga gtgcggttcg acgccaaatc gacgaaaaag
     1321 ttgcggtgac cgacctccgt caaacgattg ccatcgagga ggctttcgcc gcctccgcgg
     1381 ccgagttggc ggaagcgggc caacacggcc gcgctgcaga tcgcctcctg gatttgatgc
     1441 gatttgggaa gatgctgcag cgcggcggca ccagcacgca ttcggcacag ggagagcatg
     1501 tgcaagagcg agtcctcaag gatctacgcg atttgtcgcc gcagctatcg ccaagtgaga
     1561 gcctggcgac aatccgcgcg ctaggcaaag cgattctgat gcctgaagac gctcaagcct
     1621 tggacgcgcg cgagcatgca gtcaatgagc gggtgtatga ctggcacact cattgggtcg
     1681 ggacggttta tcgcgtgttt ccccccgagc gccgtctggt accggcctcg cgcgatacag
     1741 caaatctcat gatggcaggc gaactggcca ttcggggcgc tcaactcgga cttgcccacg
     1801 atcagttttg caacgtcacc ggtcgtccgg ctgtggccat ctctgagctc gtccccgagt
     1861 atctgcccgc cctgccactg gacccattca cccgccaacc gctgcggact tgccggaccg
     1921 ccgacgaaga cctggttatc tatagcgtcg gccccgacgg catcgacaac ggcgggaact
     1981 ttgcacgcta tgcacaccag ccgtatgaaa agcccggcta cgacatccga gtgacgttcg
     2041 actaggactc gttcggaaaa actataaggg aaactacatg gtgtcaggaa ccgaacaggc
     2101 attgtgtctt ttcggttcct ccctcgttat tcttcccaat atgcacccga agttaaatcg
     2161 ccaaagcatg ctcctgggga ccgcggtggt cgtggtcttg ctgcttgcgt tcgtggcgtt
     2221 gctggttggc atcgatcggc gatccccgtt gtcgccaatc gaggcgcgct tgatcggcga
     2281 gtggtctcac gatcctgctg aaacggtgcg atcgtttcgt gcagatcgat cattctcgac
     2341 ctccaatggg cagtttgtcg gcacctggga cgtcagggac gggcgactga cggtaaccta
     2401 ctggcagccg tttgagcttc cgacggattt cagcgcggcc gcattaacgc attcgattcg
     2461 gcggacgcgc agggagactc tgtcgtggag aatcgaattc tccgcagaca accaacagct
     2521 ctccctggat caaggttgcg tattgcaaga tggtcgcctg atccccatca gggcgggcaa
     2581 tgaaaagtgg ctgttcacgc gcgtgaggaa cgcagcgaag taaggccaga accaagccag
     2641 acgccggagc ggcggcgcga ggtctaacgg aactgctatg gcaatcggaa ctcttacgta
     2701 tgcgacccct tcgctattcc atcaacgtca cgctggacgg gtgctgcgat catcgggcag
     2761 ggtccacgga tgaagagttg catcgttttt gggccgaaaa cctcgcgcag gccgatgctc
     2821 tcctcttcgg ccgggtaacc tacgaaatga tggaggcagc atggcggccg tcggcgacgg
     2881 gcgtgaagcc ggattggatg gccgagtgga tggaaccctt cgcccggacg atcgacgcgg
     2941 ccaagaagta tgtcgtgtcg agcacgctgg accgggtcga ttggaacgca gagctggtgc
     3001 gcggggatct gggccaggcc gtcgagcaac tcaagcagca ggcgggtaac ggactgttca
     3061 cgggaggcgt gaagctcccg ctggcgctga cggagctggg attgatcgac gagtacgagt
     3121 tcgtggtgca tcccaggctc gcgggccacg ggccgacgtt gttcgcgggg ctgtcgaagt
     3181 atgttgactt gaagctcgtg gaccggttgg agttcggctc gggggcggtg gcgatgcggt
     3241 acgagccgag aaggtagccc tcgccggcgc aacctctccc acgtggtggc cggtagagag
     3301 gcactgcctc tccaccgctc cgcgcccgcc actgccggga tgaatcgaag cgccaacgat
     3361 ctcaaaccac ccgac
//
LOCUS       DASUGB010001027         5837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1781801, whole genome shotgun sequence.
ACCESSION   DASUGB010001027 DASUGB010000000
VERSION     DASUGB010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781801"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VFV87_19455"
     CDS             <1..1096
                     /locus_tag="VFV87_19455"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5106009.1"
                     /translation="VVTDPQLQAAGLVRALFPLRDANITTHFFTGGEPEPAVETAVIA
                     AEAARAFRPDCILGLGGGSNMDLAKIVAVLVTHGGEPARYFSFDNVPGPVLPLVCLPT
                     TAGTGSEVSHAAVLTDKANQIKVSTLSQYLRPAIALVDPALTDGCPRQVTADSGIDAL
                     THAIEAYTAIDFDELDAPDGAPVAYEGRYPLGECLAEKAIDLIGQHLITAVQDGKNTA
                     ARDGMALAATLAGLAFSNCAVALVHALEYPIGGAVHVSHGAGNGLLLPYVMRYNLPVR
                     AAAFAKIAALLGEKTSGLSEPAAAERSIAAVERIRAAIGIPQRLRDLGVRREQLPGFA
                     AKAFAIKRLMGTNPRQPTEPDLLGILEAAY"
     gene            1228..>5837
                     /locus_tag="VFV87_19460"
     CDS             1228..>5837
                     /locus_tag="VFV87_19460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5106010.1"
                     /translation="MYRLSLTALALLIVAPAFAADGNRLAYLDGPLDPYYPHRDFPKL
                     ITPQWVGEEGVECVVTLAIDDMRDTAKYEAFLRPILDRLKKIDGRAPLSIMSCRVDPK
                     DPQLQAWLKEGLSIEVHTFDHPCPCLQDGGLVQAKETYDKCVDLMNEIPGNKPVAFRM
                     PCCDSKNTPSPRFWAEVFNKTTAQGNFLQIDSSVFNIITSNDKDLPKEITTLPDGSER
                     FRRYVPFENFVNTIRDYPYPYVIGGMCWEFPCVVPSDWSAQHVQKPNNPDTLRDWKLA
                     LDACVFKQGTFNLVFHPHGWIRNDQIVELIDHAVSKHGKKVKFLTFKECAERLNENLL
                     NGTPLRVPTGLQSEARLADLNNDGFIDVDLIWTESRVAQRKWDAKSKSWVSSERGQEN
                     DLVDELLKKARKTLFFRDVDGDGQSESIKSGAGVIVVIRSDKLTDDNAIKLPARVAFN
                     RTDEDTGLRLIDINADGKLDCLFSNHERYSLHLFKDMKEGWATRVFDVERGKEDASGA
                     PAILPFVRADGTNNGAFFHSGALWFVNEDTWRLPDHVQKFTFAELLAPLGQKDSSPQR
                     KQGKSGPMPRGNVDSPSLALRATIETPPPKSPDEALKSMHLKPGFKIELVAAEPLVVD
                     PVAFDWGPDGRLWVVEMRDYPNGITWKKPGDEFGVPGGRVKVLTDTDGDGKYDKASVF
                     LDQIPFPTGVKVWRKGILVTAAPDIFYAEDANGDDVADERKVLYRGFGEGNQQHRVNG
                     LRWGLDNWLYIGNGDSGGVIKALAALAPTRSVSEGGTPTRRASEGTEVNVSGRDLRIR
                     PDTGEIDAQSGNTQFGRERDDWGNWFGGNNSNPMWHYTLEDQYLRRNPHFAPPLSKKQ
                     VSVSPGAAPVFPKSKTLARFNDFSAADRFTSACSPIIYRDELLGPEFYGNSFVCEPVH
                     NLVHREIVTAEGTTFTSRRADDEQTSEFLASDDNWFRPSMCRTGPDGALWVSDMYRFV
                     IEHPKWIPDEFQRTLDMRAGGNKGRIYRIYAENTPPRPIARLDKLDTAGLVAALESPN
                     GQQRDLAQQMLIWQNDRKSAGPLLMNLIMTSERPQTRLQALCALDGLLATPATLFAHA
                     LADEHPAVRRHAVRLTEPRLGKSDLGDAVLKLASDPDPHVRMQVAYSLGAWNDEQAAF
                     ALADLLLANRDDPYLTAAAMSSLNKENAGAVLARVLAVEGMEPPAELVEKVLSCAMAL
                     GEGTAVRAATDLAVAPRPGGPQLWQLAVMGRLFDELDRRKVELATLTGETAKGDFKAL
                     LDAARTWARGEGRGQDEAHRTAAIRLLARGLDGRDEDFALLGSLLAPQQPASVQAAAV
                     AAISRLRRPDVAAALLAGWSSHTPALRQQILDMLLSREDWSGELLEAIESGKVSASQL
                     DARRRQQLLTAKSDAIRQRAEKALAGAVDANRQKIVEQYLKAAAEVKTDATRGKAVFA
                     KRCANCHRLEGVGHSLGPDLAALTNRSREFLLTAVFDPNRAVEDKFLEYVARLTDGRT
                     VNGMLFEETGASLTLAAPEGKQAVIPRSEVEELKSSGKSLMP"
BASE COUNT         1118 a         1939 c         1840 g          940 t
ORIGIN      
        1 tgttgtcacc gatccgcagc tgcaggctgc tggactggtg cgggccctgt ttccgttgcg
       61 cgacgcaaat atcacgacac atttcttcac cggcggcgag ccggagccgg cggtggaaac
      121 ggctgtcatc gcggcggagg ctgcgcgtgc attccggccc gattgcatcc tgggactcgg
      181 tggcggcagc aacatggacc tggcgaagat cgtcgcggtc ctggtcacgc atggcggcga
      241 gccggcccga tatttcagct tcgacaatgt ccccggcccc gtcctgccgc tcgtctgcct
      301 gccgacgacc gccggcaccg gcagcgaggt ctcgcacgcc gcggtgctca ccgacaaggc
      361 caaccagatc aaggtcagca cgctcagcca gtacctgcgg ccggcaatcg ccctcgtcga
      421 tccggccctc accgacggct gccccaggca agtcacggcc gacagcggga tcgatgcgct
      481 cacgcacgcg attgaggcct acaccgccat cgacttcgac gaattggacg cgccggacgg
      541 cgcgccggtc gcctacgaag gccgctatcc gctgggcgaa tgcctggcgg agaaggcgat
      601 tgacctgatc ggccagcacc tgattacggc ggtgcaagac ggcaaaaaca cagcggcccg
      661 cgacggcatg gcgctggccg ccacgctcgc cggcctggcc ttctccaact gcgcggtcgc
      721 cctggtccac gcgctggagt atcccatcgg tggcgcggtc catgtctcgc acggcgcagg
      781 caacggcctg ctgttgccct acgtgatgcg ctacaacctg cctgtccgcg ctgccgcgtt
      841 cgccaaaatc gccgccctcc tcggcgagaa aacctctggc ctttccgaac ctgccgcagc
      901 agaacgatcc atcgctgccg tcgagcgcat tcgcgccgcc atcggcatcc cgcagcgcct
      961 ccgcgacctg ggcgtccggc gcgagcaact cccgggcttc gccgccaaag ccttcgccat
     1021 caaacgcctc atgggcacca acccccgcca acccaccgaa cccgacctcc tcggcatcct
     1081 cgaagccgcc tactgactcc ctctcccttt gggagagggc ggggtgaggg tgcctgccgt
     1141 atctctgcgg tctccgcgcc tctgcgtgag atttgtccgc ccctgggtta cgataacggc
     1201 acgccctcac ttcccgggag tgtcgccatg taccgactca gcctgactgc cttggctcta
     1261 cttatcgtgg ctccggcgtt tgccgctgat ggcaatcggc tggcgtatct ggatgggccg
     1321 ctggacccgt attacccgca ccgcgacttt cccaagctga tcacgccgca gtgggtgggc
     1381 gaggaggggg tcgagtgcgt cgtcacgctg gcgatcgacg acatgcgcga caccgccaag
     1441 tacgaggcct tcctgcggcc gatcctggac cgcctcaaga agatcgacgg ccgcgcgccg
     1501 ctcagcatca tgtcctgccg cgtcgatccg aaagacccgc agcttcaggc ctggctcaag
     1561 gaaggcctgt cgatcgaggt tcacactttc gaccatccct gcccctgcct ccaggacggc
     1621 ggcctggtcc aggccaagga aacctacgac aagtgcgtcg atctgatgaa cgaaatcccc
     1681 ggcaataagc ccgtggcgtt ccggatgccg tgctgcgaca gcaaaaacac gcccagcccc
     1741 cgcttctggg ccgaggtctt caacaagacc accgcccagg gcaacttcct gcagatcgac
     1801 agcagcgtct tcaatatcat cacgagcaac gacaaggacc tgcccaaaga gatcaccacg
     1861 ctgccggacg gctccgaacg cttccgccgc tatgtgccgt ttgaaaactt cgtcaacacg
     1921 attcgcgact atccctatcc ctacgtgatc ggcggcatgt gctgggagtt cccctgcgtg
     1981 gtccccagcg actggtcggc ccagcacgtc cagaagccga acaaccccga caccctccgc
     2041 gactggaagc tcgccctcga cgcctgcgtc ttcaagcaag gcaccttcaa tctcgtcttc
     2101 cacccccacg gctggatccg caacgaccag atcgtcgagt tgatcgacca cgcagtcagc
     2161 aagcacggga agaaggtgaa gttcttgacg ttcaaggagt gcgcggagcg gttgaatgag
     2221 aatcttctca atggaacacc gcttcgcgta ccaaccgggt tgcaatcaga ggcgcggttg
     2281 gcggatctaa acaacgacgg cttcatagac gttgatctca tttggactga gtcacgagta
     2341 gctcaacgaa aatgggatgc aaagtctaaa tcgtgggttt caagcgagcg gggtcaagag
     2401 aatgacttgg tcgatgaatt gctgaagaaa gcgaggaaga ctctcttctt tcgtgacgtt
     2461 gacggcgacg gtcaatccga atcgatcaaa tcgggagctg gtgtaattgt cgtgattcgg
     2521 tccgacaaac tgacggacga caatgcaatc aaactccccg ccagagtcgc cttcaaccga
     2581 accgatgaag atacgggact tcgcctgatc gacatcaacg ccgacggcaa gctcgattgc
     2641 ctcttctcca accacgaacg ctattcgctc cacctcttca aggacatgaa agagggctgg
     2701 gctacaagag tgttcgatgt cgagcgcggg aaggaggacg cgagcggcgc gccggcgatt
     2761 ctgccgttcg ttcgggccga cggcacgaac aacggcgcgt tcttccactc cggcgcgctc
     2821 tggttcgtca acgaagacac ctggcgcctg cccgaccacg tgcagaagtt cacgttcgcc
     2881 gagctgctgg cgccgctggg ccaaaaggat agtagcccgc agcgcaagca agggaaatcc
     2941 gggccaatgc cgcgaggcaa cgttgattca ccctcgcttg cgctacgggc tacgatcgaa
     3001 acgccgcccc ccaagtcgcc cgacgaggcg ctcaagtcga tgcacctcaa gcccggcttc
     3061 aagatcgagc tggtcgccgc ggagccgctg gtggtcgatc cggtggcgtt cgactggggg
     3121 ccggacgggc ggctgtgggt ggtcgagatg cgggactatc ccaacggcat cacctggaag
     3181 aagccggggg atgagttcgg cgtgcccggc gggcgcgtga aggtgctgac cgacaccgac
     3241 ggcgacggca aatacgacaa ggcgagcgtg ttcctcgacc agattccgtt tccgaccggc
     3301 gtgaaggtct ggcggaaggg aatcctcgtg acagccgcac cggacatttt ctatgccgag
     3361 gacgccaacg gagacgacgt agccgacgag cgaaaggtcc tctaccgcgg ttttggcgag
     3421 gggaaccagc agcaccgggt gaacggcctg cggtggggac tggataactg gctctacatc
     3481 ggcaacggcg acagcggcgg tgtaatcaag gcgctggccg ccctcgcacc aacccgaagc
     3541 gtaagcgagg gaggcacacc aacccgacgc gccagcgagg gaaccgaggt caacgtcagc
     3601 ggccgggacc tgcgaatccg cccggatacg ggcgagatcg acgcccagtc cggcaacacg
     3661 caattcggcc gcgagcggga cgactggggg aactggtttg gcgggaacaa cagcaacccg
     3721 atgtggcatt acacgctgga ggaccagtac ctccgccgca acccgcactt cgccccgcca
     3781 ctcagcaaga agcaggtgag cgtcagcccc ggcgcggccc ccgttttccc gaagagcaag
     3841 acgctggccc gcttcaacga tttttcagcc gccgaccgct tcacgtcggc ctgcagcccg
     3901 atcatttacc gcgacgaatt gctcgggccg gaattctacg gcaacagctt cgtctgcgag
     3961 ccggtgcaca acctggtgca tcgcgagatc gtcacggccg aaggcacgac gttcaccagc
     4021 cgccgggccg acgacgagca aacgagcgaa ttcctggcct cggacgacaa ctggttccgt
     4081 cccagcatgt gccgcaccgg ccccgatggg gcgctgtggg tcagcgacat gtaccgcttc
     4141 gtgatcgagc atcccaagtg gattcccgac gagttccagc ggacgctcga tatgcgggcc
     4201 ggcggcaaca aaggccgcat ctatcgcatt tacgccgaga atacgccccc gcggccgatc
     4261 gcccgattgg acaagctcga tacggcggga ctggttgcgg ccctcgaatc gcccaatggg
     4321 cagcagcggg atctcgcgca gcagatgctc atctggcaaa acgatcgaaa atccgccggg
     4381 ccgctgctga tgaacctgat catgaccagc gagcggccgc agacccgctt gcaggcgctg
     4441 tgtgccctcg acggtctgct cgccacgccg gcaacgctct ttgcccacgc actggccgat
     4501 gagcatcccg ccgtccgccg tcacgccgtc cgcctgaccg aaccgcgatt gggcaagtcc
     4561 gatttgggcg atgccgtact gaagctggcc agcgatcccg atccgcacgt acgaatgcag
     4621 gttgcctatt cgctcggcgc ctggaacgac gagcaggcgg cgtttgccct ggccgacctg
     4681 ctgctggcca atcgcgacga tccctatctg accgcggcgg cgatgagctc gctgaacaag
     4741 gagaacgcgg gagccgtgct ggcccgcgtg ctggcggtgg aagggatgga gccgcccgcg
     4801 gagctggtcg agaaggtgct gagctgtgcg atggcgctgg gcgaagggac ggctgtgcgt
     4861 gcagcgaccg acctggccgt cgcaccgcgg cccggtggtc cgcaactctg gcaacttgcc
     4921 gtgatgggcc ggctgtttga tgaattggac cggcggaagg tcgaactggc gacgctcacc
     4981 ggcgaaactg cgaagggtga tttcaaggca ttgctcgacg ccgctcgcac ctgggctcgc
     5041 ggcgaaggcc ggggccagga cgaggcccac cgcacggctg ccattcgcct gctggcccgc
     5101 ggtttggacg gccgcgatga ggatttcgcg ctcctgggct cgctgctcgc cccccagcag
     5161 ccggcgagcg tccaggcggc ggcggtcgcg gcgatctcgc ggctgcggcg gccggatgtc
     5221 gccgcggccc tcctggcagg ctggtcgtcg cacacgccgg ccctgcggca gcagatcctg
     5281 gacatgctcc tcagccgcga ggactggtcg ggcgagctgc tggaagcgat cgaaagcggc
     5341 aaagtgagcg ccagccagct cgatgcccgc cgccggcagc agcttttgac cgcgaaaagt
     5401 gatgcgattc gccagcgggc cgagaaggca ctggccggcg ccgtcgatgc aaaccggcag
     5461 aagatcgtcg aacagtatct gaaggcagca gccgaagtca aaacggacgc cacccgcggc
     5521 aaagcggtct tcgccaagcg ctgcgcgaac tgccaccggc tggaaggggt cggccattcg
     5581 ctcgggccag acctggcggc gctgaccaat cgctcgcgcg aattcctgct gacggccgtg
     5641 tttgatccga accgcgccgt ggaggacaag tttctcgaat acgtcgcccg gctgaccgat
     5701 ggccgcacgg tgaacgggat gctgttcgaa gaaaccggcg cgagcctgac cctcgccgcg
     5761 ccggaaggca agcaggcggt cattccgcgg agcgaagtcg aggagctcaa gtcgagcggc
     5821 aagtcgctga tgcccga
//
LOCUS       DASUGB010001028         5182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_391605, whole genome shotgun sequence.
ACCESSION   DASUGB010001028 DASUGB010000000
VERSION     DASUGB010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391605"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1474)
                     /locus_tag="VFV87_19465"
     CDS             complement(113..1474)
                     /locus_tag="VFV87_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106011.1"
                     /translation="MLTILGPRDTKTTHCDGWKRRDFLKIGGMVMGGLSLPQLLSAEA
                     QAGIGQSHKAIVNVFLPGGPAHQDFWDIKVDAPAEIRGEFQPIKTNVSGIEIGEIFPK
                     IAAIADKCVFIRTMVGATGGHDAYQCMTGRPLNPAPAGGWPAAGAWVSKLKGPVNNTI
                     PPHLSMCYKTDHAPWGYDGDGGFLGIAHAPFKLVGGKSETSKTENMVLNGITLDRLGD
                     RTGLLGSFDKFRRDLDTSGKMKGLDTFTQQAVGILTSSTLADALDISKEDPALVEKYG
                     KGDPKFRDDGAPKLTENFLIARRLVEAGARVVSLNFSRWDHHGQNFKAIRDDGPLLDR
                     ATAALITDLHERGLDKDVSVVVWGEFGRTPKINGSAGRDHWPQVSCALLACGGMRTGQ
                     VIGATNRLGEHAVERPVSFQEVWATLYHNLGLNLRAIREFDLRGRPQYLVDEGVEPLK
                     EVI"
     gene            complement(1651..4266)
                     /locus_tag="VFV87_19470"
     CDS             complement(1651..4266)
                     /locus_tag="VFV87_19470"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5106012.1"
                     /translation="MTSRSCYFVVALSVAFCFCAPFSGPAWGGEPLTFEQHIRPILKA
                     HCFHCHGEEEKHEGNLDLRLVRLMVQGGDGGPAIVPGKSAESYLIERVVAGEMPPEGK
                     GLAERELATLRAWVDQGAAIAQPEPETLSDIAETEREFWSFQPIGPPPLPTVNDSAQL
                     RSPIDAFLLAELQKRNLSFSPEADDRTLIRRLQFDLVGLPPSPDEVDAILADESPDAY
                     ERLVDRLLAQPQYGERWGRHWLDVAGYADSNGYNEADSERKYAFKYRDYVIRALNADR
                     PWDEFIIQQLAGDELLTPPYSNLAPEQADLLAATGFLRMAPDGTGEGGADPLLARNDV
                     VAETIKIVSSSLLGLTVGCAQCHNHRYDPISQVDYYRVRAIFEPAFDTKNWRPPQARL
                     VSLWSDATRAEAAAIDAELKALADERLAELDKIVMEIFDAEVAKLPEELRELARAARA
                     AEKDRTPELQQILKDYPSLKVDRGSAYLYEPKRINEFNKRFDHRQQAIKARRPAEDFV
                     PCLTEVPGQIPPTHLLYRGDVNQPKQPLEPAELSVLGAVTAIPADDPSLPTSGRRLAY
                     ARWVTSGKHPLVGRVVVNRFWLHHFGRGIVATPGDFGRLGEPPSHPQLLDWLAAEFSA
                     SGWELKRFHRLLLQSTAYRQVSTRNQRLDALDPENRLLGRMSIRRLEAEALRDSILAA
                     SGLLNSELFGEPVPVTVDEVGQVIVGLDNRDSAGRPEGGRESLGANEFRRSVYIQVRR
                     SQPLTMLATFDAATLTPNCELRNRSTVAPQSLLLMNSEFVLQQSRALAQRAIERAGDD
                     PAARVRMAWGLALSQEPSVSQIDAALAFLATYQRESASQPPPIGKTPAPEPPADGLSA
                     LCQALFSSNGFLYVD"
     gene            4665..>5182
                     /locus_tag="VFV87_19475"
     CDS             4665..>5182
                     /locus_tag="VFV87_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106013.1"
                     /translation="MRVSPSALIVCLGLGLQASGQVPHDPFAAPASSPSSAGPSQPAA
                     ASRPQPYVTRQSDVEIPFSVRPGNSPQAEPASVRVFVSWDRGKSWHFYDERRPDEGRF
                     RFRPKQDGEFWFATQTIDRSGRPDSPDPRKPQLRLIVDTQRPNLLVQAQVQPSGEVAV
                     AWSAADPTLAPTS"
BASE COUNT          947 a         1632 c         1647 g          956 t
ORIGIN      
        1 aagggtgagc atcgtttccc tgtagggtgc ataagcgaag cgcaatgcac caaattgcgt
       61 ggtgatgaca aaccggtgca ttgcggccgc ggcggcctta tgcaccctac agctagatca
      121 cttccttcag cggttcgacg ccttcatcga ccaggtattg cgggcggccg cggaggtcga
      181 actcgcggat ggcacgcagg ttgaggccca ggttgtgata aagggtggcc cagacttcct
      241 ggaagctgac gggccgctcg accgcgtgct cgcccaggcg gttggtcgcg ccaatgacct
      301 ggccagtccg catgccaccg caggcgagca gggcgcacga aacctgtggc cagtggtcgc
      361 ggccggcgct gccgttgatc ttcggcgtgc ggccgaattc gccccagaca acgaccgaga
      421 cgtccttatc gaggccccgc tcgtgcagat cggtgatgag cgccgcggtc gcacggtcca
      481 gcagcgggcc gtcatcgcgg atcgctttga aattctgccc gtgatggtcc cagcggctga
      541 agttgagcga cacgacgcgg gcgccggctt cgacgaggcg gcgggcgatg aggaagtttt
      601 ccgtgagctt tggcgcgccg tcgtcgcgga atttcgggtc gcccttgcca tacttctcga
      661 ccagcgccgg gtcttccttg ctgatatcga gggcgtcggc cagggtggac gatgtgagaa
      721 tgccgaccgc ttgctgcgtg aaggtatcga gccccttcat cttgccgctg gtgtccaagt
      781 cgcggcggaa tttgtcgaac gagccgagca gaccggtgcg gtcgcccagg cggtcgagcg
      841 tgatgccgtt caagaccatg ttttcggtct tgctcgtctc gctcttgccg ccgacgagct
      901 tgaacggagc gtgggcgata cccaaaaagc cgccgtcgcc gtcgtacccc cagggggcat
      961 ggtcggtctt gtagcacatc gacagatgcg gcgggatcgt gttattcacc ggccctttca
     1021 gcttactcac ccacgcgcca gccgccggcc agccgcccgc gggggccgga ttgagcggcc
     1081 ggcccgtcat gcattgataa gcgtcgtggc cgccggtcgc gccgaccatc gtgcggatga
     1141 aaacgcactt gtcggcaatc gccgcgatct tcggaaagat ctcgccgatc tcgatcccgc
     1201 tgacgttcgt cttgatcggc tggaactcgc cgcggatttc cgccggggca tcgaccttaa
     1261 tgtcccaaaa gtcctgatgt gccggtccgc ccggaaggaa gacattgaca atcgccttgt
     1321 ggctctggcc aatccccgcc tgggcctccg ccgacaaaag ctgcggcagc gagaggccgc
     1381 ccatcaccat gccgccgatc ttgagaaaat cgcggcgctt ccagccgtcg cagtgcgtcg
     1441 tcttcgtgtc gcgcgggccg agtatcgtca gcataagact ctcctagtcg cgaaatcgca
     1501 gggcaggttc agcacctgcc aatcaaggtg ggcatgactc gctcgagcca tcaggcgggc
     1561 agccgggcgc tccgcacgag cggattaacc cagcttctgg aaatcatatc ggaaccgatt
     1621 gatcggttca agcgtttttg tttcgtcgga tcaatcgacg tacaagaaac cattggaact
     1681 aaacagggcc tgacagagag cggacaggcc gtcggcaggc ggctctgggg ccggcgtttt
     1741 gccgatcggt ggcggttggg atgcagactc ccgctgatat gtcgccagga acgccagagc
     1801 cgcgtcgatc tgagacacgc ttggctcctg tgaaagagct agcccccatg ccatccgcac
     1861 gcgagcggcg ggatcgtcgc cagcccgctc gatggcacgt tgggcaaggg cccgcgactg
     1921 ctggagcacg aattcactgt tcatcagcag cagtgactgc ggcgcaacgg tggagcgatt
     1981 gcgaagctcg cagttgggcg tgagagttgc agcgtcgaat gttgccagca tcgtaagggg
     2041 ctgcgagcgg cggacctgga tatagacgct acgacggaac tcgttcgctc cgagggactc
     2101 tcggcctcct tccggcctgc cggcgctatc gcggttatcc agaccgacga ttacctgccc
     2161 aacttcatcg accgtcaccg gcaccggctc gccaaacaat tcagaattca agagccccga
     2221 cgccgcgagg atcgaatcgc gcagtgcctc ggcctccagg cggcgaatcg acatgcggcc
     2281 gagcaggcgg ttttccggat cgagcgcgtc gagtcgctga ttgcgtgtgg atacctgccg
     2341 atacgcagtg gattggagca gcaaacggtg gaagcgcttc aattcccagc cactggccga
     2401 aaactcagcc gcgagccaat cgagcaattg gggatgcgag ggcggctcgc cgagccggcc
     2461 aaagtctcca ggcgtggcga caatcccgcg gccgaagtgg tgcagccaga agcgattgac
     2521 caccacgcgg ccgacgagcg gatgcttgcc actcgtgacc cagcgggcgt atgccaacct
     2581 tcggccggag gtcggcagcg agggatcgtc agcaggaatt gccgtaaccg ctcccaacac
     2641 gctcagttct gccggctcca gcggctgctt cggctgattg acgtcgccgc gatacaacag
     2701 atgcgtcggc ggaatctgac cgggaacttc ggtcaagcag gggacgaagt cttccgcggg
     2761 gcggcgcgct ttgatcgctt gctgccggtg gtcgaaccgt ttgttgaatt cattgatccg
     2821 cttgggctca tacaggtacg cgctgccgcg atcgaccttg aggctcggat aatccttgag
     2881 aatctgctgc agctcgggcg tgcggtcctt ttcggcggcg cgggcggcgc gagccaattc
     2941 gcgaagctcc tcgggcagct tggcgacttc ggcgtcgaat atctccatca cgatcttgtc
     3001 cagttcggcc agccgctcat cggctagggc cttgagctcc gcgtcgatag cggctgcttc
     3061 cgcccgggtg gcatcgctcc agagcgaaac gaggcgggcc tgcggcggtc gccagttctt
     3121 tgtatcgaac gctggctcga agatcgcccg cacgcgatag tagtcgacct gcgaaatcgg
     3181 atcgtagcga tggttgtggc actgggcaca gccgaccgtc agacccagca gcgaagaact
     3241 tacaatcttg attgtctcag caacgacgtc gttgcgagcg aggagcggat cggctccgcc
     3301 ttcaccggtg ccatccggcg ccatccttaa gaagccagtt gccgctaaga ggtcggcctg
     3361 ttctggtgcg agattcgagt agggcggcgt gagcagctca tcaccggcaa gttgctggat
     3421 gataaattca tcccacggcc tgtcggcgtt gagcgcccgg atgacatagt cgcggtactt
     3481 gaaggcgtac ttccgctcgc tgtcggcttc attgtagccg ttgctgtcgg cataccctgc
     3541 gacgtcgagc cagtgccggc cccagcgctc gccatactgc ggctgcgcga gaagccgatc
     3601 gaccagccgc tcgtaagcat cgggcgactc atcggctagg attgcatcga cctcgtcggg
     3661 agacggcggc agcccgacca gatcgaactg gagccggcgg ataagcgttc ggtcgtcggc
     3721 ttccggcgag aagctcaagt tgcgcttctg cagctctgcg aggagaaagg cgtcgatggg
     3781 agagcgcagc tgggcagaat cgttcaccgt cggaagtggc ggaggcccaa tcggctggaa
     3841 cgaccagaat tccctttccg tttccgcaat gtcggacagt gtctcgggct ccggctgggc
     3901 aattgctgca ccttgatcga cccaggcgcg gagcgtggcg agttcccgct ccgccaatcc
     3961 tttcccctct ggcggcattt cgcccgccac gacgcgctcg atcaaataac tctccgccga
     4021 tttgcccggc acaatcgccg gcccgccgtc gcccccttgc accatcagcc gtaccagccg
     4081 gagatcaaga tttccctcgt gtttctcttc ctcaccgtgg cagtggaaac aatgagcttt
     4141 caaaattggc ctgatgtgct gttcgaacgt cagcggctcg ccgccccacg ctggcccgct
     4201 gaagggcgcg caaaaacaga acgccacgga caatgcaaca acgaaatagc acgagcggct
     4261 cgtcatggtc acttgccagg taggattaga ggagtgaccg gttcaggcag gcgttgacta
     4321 attgtggcca atctgctgac caggtgcaac tgaacaagct ctggtgcagg ctaggaggcc
     4381 gggatgcaat tccgacctgt cgacgcagcc ttcgtgaacc acagcgatcg gctggcacac
     4441 tcagccctac atgttcccca aattgcctga ctggtgctaa tcgggcgggc ggtataggtg
     4501 ctagagccga ttgcccgatg agagccaaga acggcgggcg gaggaccagc gctcgcgctc
     4561 agccgcgcgc aggatcgtgc cgccggttgg gtggcagaca ggaaagtctg ccccacgtgt
     4621 cgtttgccgc aggacgcggt attccatcgc cagggacggc cagcatgcgc gtgtcgccat
     4681 cagcgctaat cgtctgcttg gggctggggc tccaggccag cggtcaggtg ccgcacgatc
     4741 cttttgcggc gccggccagc tcgccatcct cagcaggtcc gagccagcct gccgccgctt
     4801 cgcggcccca gccgtacgtg acgcggcaaa gcgatgttga gattcccttc tcggtgcgac
     4861 cgggaaactc accccaagca gagcccgcct ccgtccgcgt ctttgtctcc tgggaccgcg
     4921 gcaaatcgtg gcacttctac gacgagcgcc ggcccgacga ggggcgcttc cgcttcagac
     4981 ccaagcagga cggcgaattc tggttcgcca cgcaaacaat cgaccgctcg ggacgccccg
     5041 acagccccga tccgcgaaag ccgcagcttc gcctgattgt cgatacacag cggccaaacc
     5101 ttctggtgca ggcgcaggtc cagccctccg gcgaagtcgc ggtcgcctgg agcgcggccg
     5161 atcccacgct cgctccgacg tc
//
LOCUS       DASUGB010001029         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1363963, whole genome shotgun sequence.
ACCESSION   DASUGB010001029 DASUGB010000000
VERSION     DASUGB010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363963"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..598)
                     /locus_tag="VFV87_19480"
     CDS             complement(98..598)
                     /locus_tag="VFV87_19480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5106014.1"
                     /translation="MTIRTLTADDAEIYRHQRLAALAEHPLAYVTDYSEEAALPLEEF
                     QKRLAAETSVTFGAFDAERLAGISTLLTTTRLRQRFRATIVGMHVLPEYRRRGIATQL
                     LSACVARARELPEVEEVCLCITVGNDAARAAYVAFGFEPELIEPRYFKYEGRYYDLEW
                     LRLTLR"
     gene            complement(666..938)
                     /locus_tag="VFV87_19485"
     CDS             complement(666..938)
                     /locus_tag="VFV87_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106015.1"
                     /translation="MSQRTRMCVICKKLIEPERAAAMASTNLCTEHGEAIERFGGEFR
                     VQSQGENLSKPGSLKRNPGGVKTRMVRNLRALDQLREECEKQKQGA"
     gene            complement(1125..2216)
                     /locus_tag="VFV87_19490"
     CDS             complement(1125..2216)
                     /locus_tag="VFV87_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5106016.1"
                     /translation="MTIGFGIIGCGMISRFHAQAIADIKGAKVTACFDSFTSSADKFG
                     AEIGCTVYHDLDKLLADKNVDVVTICTPSGVHMEPAVAAAKAGKHIIVEKPLDITLKR
                     CDAMIAAAKKAGVVLSTVFPSRFHESSQLLKGAIDKGRFGKLTMGDAYVKWFRTQEYY
                     DSGAWRGTWALDGGGALMNQAVHSVDLLLWFMGPAVEVSAYTATLAHQRIEVEDVAVA
                     NIKFASGALGVIEASTATFPGELKRIEVSGDKGSVVLREEDLAVWKFAKETKADEAIR
                     EKMASRTHTGGGAADPKAIGYHGHRLQFQDVLKAIKTGKQPLVDGPEGRKAIELILAI
                     YKSAETGKPVKLPLSGDPPLKARSKGKKL"
     gene            complement(2316..>2967)
                     /locus_tag="VFV87_19495"
     CDS             complement(2316..>2967)
                     /locus_tag="VFV87_19495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5106017.1"
                     /translation="APPVYALQGVLVYFWKKSEHPLMEMFKTTPDAAFFATLIAAAVL
                     VAPLVEETLFRVLLQGFLEKVVSSRGKVLGLLLGRFSFLETVEPPIRPDQPGIPPPVD
                     PTRDVNPYEPPQIGPHFDPPLPEVAAVISDDQPELRGPQAWIPIGVSALIFALLHYSH
                     GPDWVPLIFLAAGMGYLYQRTHSIVPSLVVHMCLNGLSMWGLWIQVYEMPGLGARG"
BASE COUNT          536 a          978 c          897 g          556 t
ORIGIN      
        1 aaagcgtcgt caaccactcg aagagcggtt gaagaagctg gagcagccgc ccaaggttcc
       61 ctagccggcg ccaaacctgg tcccggcgtg tcggcgccta tcgcagagtc agacgcagcc
      121 actccagatc gtaataacgc ccttcgtact tgaagtacct cggctcgatc agctccggct
      181 caaatccgaa ggcgacatac gccgcacggg cggcgtcgtt cccgacggtg atacagaggc
      241 aaacttcctc gacctccggc agctcgcggg ctcgcgcgac gcacgccgac aaaagctgcg
      301 tggcaatccc gcggcgtcga tattctggca gcacgtgcat tcccacaatc gttgcccgga
      361 accgctgccg caaacgagtg gtcgtcagca gcgtggaaat gccagccagc cgctctgcgt
      421 caaacgcacc gaacgtgacg ctcgtctccg ccgccaggcg cttctggaac tcttccagcg
      481 gcagggccgc ctcttcgctg tagtccgtca cgtacgccag cggatgctca gcgagcgccg
      541 ccagcctctg gtggcgataa atctcggcat catccgccgt gagagtgcga attgtcactg
      601 gaaggtctgg catatcgaat cgcctgccac tcccagacac cagccgatgc caagggttcg
      661 ccgtatcagg cgccctgctt ctgcttctcg cactcctctc gcaactggtc aagggcccgg
      721 aggttgcgga ccatgcgggt cttcacgcct cccgggtttc gtttcaggct gccgggcttg
      781 ctgaggtttt ccccctggct ctgaacgcgg aattcgccgc cgaatcgctc gatggcctcg
      841 ccgtgctcgg tgcacagatt cgtgcttgcc atcgccgccg cccgctcggg ctcgatcaat
      901 ttcttgcaga tgacacacat ccgcgtgcgt tggctcatgc cggctccttt ctctatggga
      961 tatccaattc accgctcgcg cgtaaaaaaa ggaccgtcgc cgggcgaccg tccttccaaa
     1021 cgtcaggttg tcggttgatc tcgtcaacct tctagacgaa acatacggcg atttggttcc
     1081 gcgggcaagc cggattctgg cgactcgctg gcggcgacct ggatctacag cttcttcccc
     1141 ttcgaccgcg ccttgagcgg cggatcgccg gatagtggca gcttgacggg cttgcccgtc
     1201 tcggcggatt tgtagatggc gaggatcagc tcgatcgcct tgcgcccttc ggggccatcg
     1261 acgagcggct gcttgccggt tttgatcgcc ttcagcacgt cctgaaactg cagcctgtgc
     1321 ccgtgatagc cgatcgcctt cggatcggct gcgccgccgc cggtgtgggt gcggctggcc
     1381 atcttctcgc gaatcgcttc gtcggccttc gtctccttgg cgaacttcca gacggcgagg
     1441 tcttcttcgc ggaggacaac gctccccttg tcgccgctga cttcgatccg cttcagctcg
     1501 ccgggaaacg tcgccgtgct ggcttcgatc acgccgagcg cgccgctagc gaacttgatg
     1561 ttggcgacgg cgacgtcttc gacctcgatc cgctggtggg cgagcgtcgc cgtgtaagcc
     1621 gagacctcga ccgccgggcc catgaaccag agcaagagat cgacgctgtg gaccgcctga
     1681 ttcatcagcg ccccgccgcc gtccagggcc cacgtgccgc gccacgcgcc gctgtcgtaa
     1741 tactcctgcg tgcggaacca tttcacatag gcgtctccca tcgtcagttt gccgaagcgg
     1801 cccttgtcga tcgcgccttt caagagctgt gacgattcgt ggaaccgcga ggggaagacc
     1861 gtcgaaagca cgacgcctgc cttcttggcc gccgcgatca tcgcgtcgca ccgcttgagc
     1921 gtgatatcga gtggcttctc gacgatgata tgcttgcccg ctttcgcggc ggcgacggcc
     1981 ggctccatgt ggacgccgct gggagtgcag atcgtgacga cgtcgacgtt cttgtcggcc
     2041 agcagcttgt ccaggtcgtg atagacggtg cagccgatct ccgcgccgaa cttgtcggcc
     2101 gacgacgtga acgaatcgaa gcaagcggtg actttcgcgc ccttgatatc cgcgatcgcc
     2161 tgggcatgga accgcgagat catgccgcag ccgatgatgc cgaaaccgat ggtcatggga
     2221 agctcctcga gggtgaaagc cagtgtgtaa cggatcgggg gtctcgggga taggggctcg
     2281 ggactcacta acgctgggca aactaccctc agcccctaac cccgagcccc gagtcccggc
     2341 atttcataca cctggatcca cagcccccac atcgacaggc cgttgaggca catgtgaacg
     2401 acaaggctcg gcacgatgct atgcgtccgc tgatacaggt agcccatacc ggcggcgagg
     2461 aagatcaagg gcacccaatc gggaccgtgc gaatagtgca gcagggcgaa gatcagcgcg
     2521 ctgacgccga tgggtatcca agcttgcggg ccgcgcagct cgggctggtc gtcgctgatg
     2581 acggccgcaa cttcgggcaa cggaggatcg aagtgcggac cgatctgcgg cggttcatat
     2641 ggattcacat cccgcgtcgg atcgaccggc ggcgggatgc caggctgatc cggcctgatt
     2701 ggcggctcga ccgtttccaa gaacgagaag cgccccagca gcaaacccag caccttgccg
     2761 cgagagctga ccaccttctc cagaaagccc tgcagcagca cgcgaaacag cgtctcctcc
     2821 acgagcggcg cgaccagcac cgccgcggcg atgagcgtcg caaagaacgc cgcatccgga
     2881 gtggttttga acatctccat cagcgggtgc tcgctcttct tccagaaata gaccagcacg
     2941 ccctgcaggg catagacggg cggcgcg
//
LOCUS       DASUGB010001030         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1086656, whole genome shotgun sequence.
ACCESSION   DASUGB010001030 DASUGB010000000
VERSION     DASUGB010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086656"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2265)
                     /locus_tag="VFV87_19500"
     CDS             complement(<1..2265)
                     /locus_tag="VFV87_19500"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5106018.1"
                     /translation="MLRTAAVFLFGLALARPFFAARQEEFDERQPLHAILLVDNSLSM
                     GYESLEGTLLDKAKDRARQLIDKLPAGSRISIIPACGSREGISADPYDTKENALEALG
                     RIEIVDRSASLVRAANEAKRASEAAPQLAKRIVFISDQQELNWRDARQGEAIKELPAI
                     QVVDVGPADWENTWISDLRVQDGLADVETPTTIIVELQHHGPSARRDVQVTLAMGDAV
                     IGEKSVTIEPGLGAREVDFEYVFNTITDLPEPDKPVFVPLKASITPDRLAADDQRFLA
                     APVVASLPVVFIDQYGPEREDAIAGRLGETRHLRKLLAPKTSRSDAPRQLISVRHITP
                     DDLNQEVLADARLVIVAGVQSPQGMVELLHDYVKQGGRVVLAAGANFDPQSWNDAAWL
                     DGEGILPLPLERQPIGEVPEVAGANVRPFFLSFESLANEPYFQLAGVAEAELRDLYAE
                     PFFFKAVHVDASPLAVERFEQAESQRMKLAEEQNSALAKRRDELEAKERQGELAVVER
                     QQLDDVRQELRTSGAAWLTWAAGVEVSAPQAVQMPRVLARYELDERPPFLVSRKIGRG
                     EVVFCSTGLLSSWNTLPKSNTVLIFDRILRGMAQQTLPRRNYEATERITLPLPPGEQH
                     LTATLVRPGSGESPEPLDVNNIGPDQRGVTVTGLFQRGVYRVAGFRPNLSADAALADD
                     KPVWEVPLVVSGPGEESDLAPLTRDQFEQSAASANLRWISPGEEISLAGTAIRGQTMW
                     WWLALLVLVLLLAEM"
     gene            2167..>2404
                     /locus_tag="VFV87_19505"
     CDS             2167..>2404
                     /locus_tag="VFV87_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106019.1"
                     /translation="MQRLALVEFFLPGGKKWPGQRQAKQEYGRSTEHEEDDVANLQNA
                     AVALHRLPQKVHGRPLHYFVTPTIEQVDNQRRSSA"
BASE COUNT          421 a          822 c          742 g          419 t
ORIGIN      
        1 catttctgcc agcagcagca ccagcacgag caatgccagc caccaccaca tcgtctggcc
       61 gcggatcgcg gttcccgcca ggctgatttc ttcgcccggg ctgatccacc gcaggttggc
      121 gctggccgcg gattgctcga actgatcgcg cgtcagcggt gcgaggtccg attcctcgcc
      181 cggtccgctc acgaccagcg gcacttccca gaccggcttg tcgtcggcca gagcggcgtc
      241 ggcggaaagg ttggggcgaa agcccgccac gcgatacacg ccccgctgga agaggcccgt
      301 caccgtcacg ccccgctggt cggggccgat attgttgaca tcgagcggct cggggctttc
      361 gccgctgccg ggacgtacga gcgtcgcggt caggtgctgc tcgcctggcg gcagcggcag
      421 cgtgatccgc tcggtcgcct cgtagtttcg ccgcggcagc gtttgctggg ccatcccgcg
      481 gagaatgcgg tcgaaaatca gcaccgtatt cgacttcggc aacgtgttcc acgacgacaa
      541 gagaccggtc gagcagaaaa ccacttcccc tctgccaatc ttgcgcgaca ccagaaacgg
      601 cggccgctcg tccagctcgt agcgggccaa cacccgcggc atttgcacgg cctgcggcgc
      661 ggaaacctcc acgcccgcgg cccacgtcag ccacgccgcg cccgacgttc gcaactcctg
      721 ccgcacgtca tccaactgct gccgctccac gacagcaagc tcgccctggc gttccttggc
      781 ctccaattcg tcgcgccgtt tcgccagagc agaattctgc tcttccgcca acttcatgcg
      841 ctgcgattcc gcctgttcaa accgctcgac cgccagcggc gaagcatcga cgtggaccgc
      901 cttgaagaag aacggttccg catacaggtc gcgcagctcg gcttcggcga cgcctgcgag
      961 ctggaaatac ggctcgttgg ccaggctctc gaacgacaga aaaaagggac gcacattcgc
     1021 gcccgcaact tccggcacct cgccgatcgg ttggcgctcc aatggaagcg gcaagatgcc
     1081 ctcgccatcg agccacgccg cgtcgttcca agactgcgga tcgaaattcg ctccagccgc
     1141 cagaacgact cgccccccct gcttcacata atcgtgcaac agctcgacca tcccctgcgg
     1201 gctctgcacg ccggcaacaa tcaccagccg ggcatcggcc aatacctctt gattcagatc
     1261 atccggcgta atgtgccgca cgctgatgag ctgccgcggg gcatcgctgc ggctggtctt
     1321 cggggcgagc aatttccgga ggtgccgcgt ctcgccgagg cggccggcga tcgcgtcttc
     1381 ccgctccgga ccgtactggt cgatgaacac aacgggcagc gaggcgacga ccggcgccgc
     1441 cagaaaccgc tgatcgtccg cggccaggcg gtcgggtgtg atcgacgcct tgagcggcac
     1501 gaagaccggc ttgtcgggct ccggcaggtc ggtgatcgtg ttgaaaacgt actcgaaatc
     1561 gacctcgcga gcgccgagcc ccggctcgat cgtgacgctc ttttcgccga tgaccgcatc
     1621 gcccatcgcc agcgtcacct gcacgtcgcg ccgcgcagaa gggccgtgat gctgcaattc
     1681 gacgatgatc gtggtcggcg tttccacatc ggccaggccg tcctgcacgc ggagatcgga
     1741 gatccacgtg ttttcccaat cggccggccc gacatcgacg acctgaatcg ccggcagttc
     1801 cttaatggcc tcgccctggc gggcatcgcg ccagttcagc tcctgctggt cgctgatgaa
     1861 aacgatccgc ttggcaagct ggggcgccgc ttcgctggcc cgcttggcct cgttggctgc
     1921 ccgcacgagg ctggccgagc gatcgacgat ctcgatgcgg ccgagcgctt cgagggcgtt
     1981 ttccttcgtg tcgtaagggt cggcgctgat cccctcccgc gagccgcagg ccgggatgat
     2041 cgaaatccga ctgccggcgg gcaatttatc gatgagctgg cgggcgcggt ctttggcctt
     2101 gtcgagcagc gtgccttcga gcgattcata gcccatgctc aagctgttat cgacgaggag
     2161 gatcgcgtgc agcggctggc gctcgtcgaa ttcttcctgc ctggcggcaa aaaatggccg
     2221 ggccagcgcc aagccaaaca ggaatacggc cgcagtacgg agcacgagga ggatgatgtc
     2281 gcgaatctgc agaatgcggc ggttgcgctg catcgcttgc cgcagaaagt ccatggccgc
     2341 ccattgcact actttgtaac gccgacgatt gagcaggtgg ataatcaacg gcgcagcagc
     2401 gcag
//
LOCUS       DASUGB010001031         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1782411, whole genome shotgun sequence.
ACCESSION   DASUGB010001031 DASUGB010000000
VERSION     DASUGB010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782411"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="pilM"
                     /locus_tag="VFV87_19510"
     CDS             complement(<1..965)
                     /gene="pilM"
                     /locus_tag="VFV87_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX5106020.1"
                     /translation="MAKTSAVWGIDIGRCALKALRCRVDGDAIVADGFDYIEYPKLLT
                     QPEADAPQLIKEALEQFLSRNSLKDDKVAISVSGESGLARYFKPPPVDVKKIADIVKY
                     EARQQIPFALEDVIWDFQRLPGGQEVDGFALDTEIGLFAMKREQVDKALAPYSVADIE
                     VDVIQLAPIAIYNYIAHDVAKELGEGEEYDSENPPPSQVVLSIGTDTTDLVVTNGYRV
                     WQRNIPLGGNHFTKQLSKELKLTFAKAEHLKRNPKTADDPKTIFQAMRPVFADLVTEV
                     QRSIGFFQSLDRKAKISSVVMLGNTVKLPGLVQYLSKHLGYDVKEV"
     gene            complement(958..1134)
                     /locus_tag="VFV87_19515"
     CDS             complement(958..1134)
                     /locus_tag="VFV87_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106021.1"
                     /translation="MNDSFALIYLITESRSRDYALAVGGGRFARRPGCLPVVWTATKA
                     RTNSSRRERGGLHG"
     gene            complement(1320..2258)
                     /locus_tag="VFV87_19520"
     CDS             complement(1320..2258)
                     /locus_tag="VFV87_19520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEX5106022.1"
                     /translation="MRFAVLAAADSWYARDLVRAGAGDYEIVTLPFSEISGAIGLAGW
                     TSGQSVRADRGQTGGPCYALSVTSAGQDLAEFDAVIVRTMPPGSLEQVVFRMDCLGRY
                     EAAGGVVINPARAIEAAVDKFLTSAKLQAAGLVTPRTIVCQTADDALAAFEQLGGDVV
                     LKPVFGSEGRGITRLDDEALALRAFKMLAQLGAVLYVQEYIEHEGYDIRLLLIGERVL
                     AMRRRNPLDWRTNISRGATAEPFTPDDSLIETARRAAAAVGAPLAGVDLLPGRDGTLY
                     AIEVNAVPGWKGLGKALGVDVARLVLDFVAFTVQNR"
     gene            complement(2282..3025)
                     /locus_tag="VFV87_19525"
     CDS             complement(2282..3025)
                     /locus_tag="VFV87_19525"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106023.1"
                     /translation="MHVERIEWGGLACFVVHELPEGTQPRLAVVLCHGFGASGSDLVG
                     LAQPLIALAPEIAEQVAFVFPAGPLSLDSRGIPGGRAWWMIDLNRLIYGPPPDLLERF
                     RRECPPGLPEARGALLELIAEAGARFGLTSDRFVLGGFSQGSMLATDVALRLPLPPAG
                     LAILSGALINEAEWRPLAEKRGSIAVLQSHGRQDSILPFPMGLVLHEMLLEAGTDVDF
                     IPFNGDHEIPTAVVQRLAALLKRALTPDS"
     gene            complement(3015..3317)
                     /locus_tag="VFV87_19530"
     CDS             complement(3015..3317)
                     /locus_tag="VFV87_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106024.1"
                     /translation="MKGPSERLKYDLRRLWECPTCHKRERTPGSVTFRHCLCRMKELD
                     GAPVVMRLVEDGPQRVTPPIVIPHEPLPPLPAIADDLPAGTASEPPTSPEPPTDAR"
     gene            3522..3914
                     /locus_tag="VFV87_19535"
     CDS             3522..3914
                     /locus_tag="VFV87_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX5106025.1"
                     /translation="MARPKEEQPTAGELDVLKVLWDRGPSTVRDVMDVLNESRPRAYT
                     SVMSLLNVMTDKGLLIREPQGRAFVYRPKTDRGRTLKRMVGDLLGRAFEGQASQLVAH
                     LLDQSKPTSSELDEIRRTIEAYQKVRGS"
     gene            3914..>4149
                     /locus_tag="VFV87_19540"
     CDS             3914..>4149
                     /locus_tag="VFV87_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106026.1"
                     /translation="MSLVEVLAHPVARQLTLALLHFVWQGLAVAVALVLAVELLGIRR
                     PQARYACSLAALLLMLICPLVTFWLLGVGESEIAR"
BASE COUNT          770 a         1288 c         1358 g          733 t
ORIGIN      
        1 acttccttga cgtcgtaacc caggtgcttg gacagatact gcacaaggcc cggcagcttc
       61 accgtattgc ccagcatgac taccgagctg atcttcgcct tgcggtcgag gctctggaaa
      121 aagccgatcg aacgctgcac ctcggtcacc agatcggcga agaccggccg catcgcctgg
      181 aagatcgtct tgggatcgtc ggccgttttc gggttgcgtt tcaggtgctc ggccttggcg
      241 aacgtcagct tcaactcctt ggaaagctgc ttggtgaagt gattgccgcc gagcggaatg
      301 ttgcgctgcc acacccggta accgttggtc accaccagat cggtcgtgtc ggtgccgatc
      361 gagagcacaa cttgcgaagg gggcggattc tcggagtcgt actcctctcc ctcgcccagc
      421 tccttcgcca cgtcgtgggc gatgtaattg tagatggcga tgggcgcgag ctggatcacg
      481 tccacctcga tatccgccac gctgtagggc gctagcgcct tatccacctg ctcgcgcttc
      541 atcgcgaaca ggccgatctc cgtatcgagc gcgaacccgt ccacctcttg ccctcccggc
      601 aggcgctgga agtcccaaat gacgtcttcc agcgcgaagg gaatttgctg gcgggcctcg
      661 tatttgacga tatcggcgat tttcttgaca tcgaccggcg gcggcttgaa gtagcgggcc
      721 agccccgatt cgcccgagac gctgatcgcg accttgtcgt ccttgaggct gtttcgcgag
      781 agaaactgct ctaatgcctc cttaatgagc tgcggcgcgt ccgcctcggg ctgcgtcagc
      841 agcttgggat actcgatgta gtcgaagccg tccgcgacga tcgcatctcc atcgacccgg
      901 cagcgcagcg ctttgagagc gcagcggcca atgtcgattc cccaaacggc agaagtctta
      961 gccatgaagt cccccacgct cgcgacggga gctgttggtg cgtgcttttg tggcggtcca
     1021 gacgaccggc aggcagcctg gcctgcgggc gaaacgcccg ccgccgaccg ccagcgcata
     1081 atcgcggctc cgcgactcgg tgatcagata gatgagagcg aacgaatcgt tcactgcgga
     1141 cagccacgat tcccctgttc cttgtcttct actctacaga ttccgcgcca ccaaagtcaa
     1201 tcagtttccc cgcgaaaacc gaagaatcgc tgctgcccgc ccccgcacta cctctgccgg
     1261 aaatttcggt gttccggcca agcggattgc tgacaacgtg gggcttaaat aatgggattt
     1321 cagcggtttt ggaccgtgaa tgcgacaaaa tccagcacca gccgggcgac atcgacgccg
     1381 agcgccttgc cgaggccctt ccagccagga acggcattga cttcgatcgc atagagagtt
     1441 ccgtcgcgtc cgggcaagag atcgacgccg gccaggggcg cgccgacggc ggctgcggcg
     1501 cggcgagcgg tttcaatgag cgaatcgtcg ggtgtaaaag gctccgccgt ggctccgcgg
     1561 ctgatgttgg tgcgccagtc gagcgggttg cggcggcgca tggcgaggac gcgctcgccg
     1621 atcaagagca agcggatatc gtagccttcg tgctcgatgt actcctgcac gtacagcacg
     1681 gccccaagct gtgcgagcat tttgaacgcc cgcagagcga gcgcttcgtc atcgagccgc
     1741 gtgatgccgc ggccttcgct gccgaagacc ggcttgagga cgacatcgcc gccaagctgc
     1801 tcgaaggcgg ccagcgcgtc gtcggcggtc tggcagacga tcgttcgcgg agtaacaagg
     1861 cccgcggcct gcagcttggc ggaggtcagg aatttatcga cggccgcctc gatcgcgcgg
     1921 gccgggttga tcacgacgcc gcccgccgct tcatagcgcc ccaggcaatc catgcggaag
     1981 acgacctgct ccagtgagcc ggggggcatc gtgcggacga tgacggcgtc gaattcggct
     2041 aggtcctggc cggcagaggt gacggagagt gcgtagcacg gacctccggt ctgtccacga
     2101 tctgctcgga cggactgacc ggaggtccat cctgcgagac caatggcgcc gctgatctcg
     2161 ctgaatggca acgttacgat ctcataatcg ccagctccgg cccgcacaag gtcgcgggcg
     2221 taccaactat cggcggcggc gaggacggcg aagcgcatgc ggaggggcca ggagcctgga
     2281 gtcaggagtc aggagtcagg gcacgcttca agagagcggc cagtcgctgc acgacggcgg
     2341 tggggatttc gtggtcgccg ttgaacggga tgaagtcaac gtcggtgccc gcttcgagca
     2401 gcatttcgtg cagaaccagg cccatcggga agggcaggat cgaatcttgc cggccgtggc
     2461 tttgcagcac ggcgatcgag ccgcgttttt ccgccagcgg acgccactcg gcttcgttga
     2521 tcagagcgcc ggagaggatt gccagcccag cgggcggtag cggcaatcgc agggccacgt
     2581 cggtggcaag catcgacccc tgcgagaagc cgccgaggac gaaacgatcc gaagtcaacc
     2641 caaagcgggc gcctgcctct gcgatcaatt cgagcagtgc cccgcgcgcc tccggcaatc
     2701 cgggcgggca ttcgcggcgg aagcgttcta agagatccgg cggcgggcca tagatcagcc
     2761 gattcaggtc gatcatccac caggctcgcc cgccgggaat cccacggctg tcgaggctga
     2821 gcggcccagc ggggaagacg aacgcgacct gctcggcaat ttccggggcc agcgcgatca
     2881 gcggctgcgc caggccgacc aggtccgatc ccgaggcgcc gaagccgtgg cacagtacga
     2941 ccgccagccg tggctgcgtc ccttccggca actcatggac aacgaaacag gcgagcccgc
     3001 cccactcgat ccgttcaacg tgcatcggtc ggcggctccg gggacgttgg gggctcgctg
     3061 gcggttcccg caggaagatc atccgcgatc gcaggcagcg gcggaagcgg ctcgtggggg
     3121 atgacgatcg gcggcgtcac ccgttgcggc ccatcttcaa cgagccgcat cacgaccggc
     3181 gccccgtcaa gctccttcat ccggcaaagg caatgccgga acgtcaccga accgggcgtc
     3241 cgctcgcgct tgtggcaagt cgggcactcc cagaggcggc gaaggtcgta tttcaggcgt
     3301 tcggagggac ccttcacgga ggggacagag gacaggggac aggaaactgg ggacagggga
     3361 cagcagggga aagactaagg caaaacgcgc ggtgctggta ggagcgagac acgtccgaag
     3421 atatctacgg ttttaaccgt tgacaactac ggcataaaca gtagaatgag cgtcgtgacg
     3481 ctttcgcgtt cgtcgaatcc acacggtaca ctgcacacgc catggccaga cccaaggaag
     3541 aacagccgac cgccggtgag ctcgacgtgc tcaaggtcct ctgggaccgt gggccttcga
     3601 ccgtccgcga cgtgatggac gtgctcaacg agtctcggcc gcgtgcctat acctcggtga
     3661 tgagcctttt gaacgtgatg accgacaagg ggctgctcat ccgcgagccg cagggccggg
     3721 cgttcgtcta tcggccgaag accgaccgcg ggcggacgct caagcgaatg gtgggcgacc
     3781 tgctgggccg ggccttcgag ggccaggcga gccagctcgt tgcccacctg ctcgatcaga
     3841 gcaagccgac gtcctccgag ctggacgaaa tccgccgcac gattgaggcc tatcagaaag
     3901 tgcgaggtag ctgatgtcgc tcgtcgaggt gctcgcgcat cctgttgcgc gccaattgac
     3961 cttggccctg ctgcattttg tgtggcaagg gttggcggtg gccgtcgcct tggtgctcgc
     4021 cgttgaatta ctcggcattc gccgcccgca ggcgcggtat gcgtgctcgc tggcggcgct
     4081 gttgctgatg ctaatttgcc cgctcgtgac gttttggttg ctgggagtgg gtgagagtga
     4141 aatcgcacg
//
LOCUS       DASUGB010001032         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2060141, whole genome shotgun sequence.
ACCESSION   DASUGB010001032 DASUGB010000000
VERSION     DASUGB010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060141"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VFV87_19545"
     CDS             <1..1004
                     /locus_tag="VFV87_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106027.1"
                     /translation="KQQSMSIEQSSHVAPYPDSPAARDRWILDRRPARLPVNPAQPNA
                     CFIEQERDVSGEIVPVATLFLTNRECPWRCLMCDLWKHTLTVSVAPGSIPQQIDHALQ
                     ALTAHAAAAPRQIKLYNSGSFFDARAIPPEDHPAIADRVRGFERVIVECHPALISERI
                     LPFRDRIGTQLEIALGLETVHPEILPRLNKRMTAAQFAEAAGFLKTHGIALRVFVLVK
                     PPFLAESDACLWAGRSIDFAYDCDAAVVSLIPTRLGNGALEALAATNEFSPPRLDTFE
                     AAVDYGMGLQRGRLFADLWDLQQFATCATCLPDRQERLNRMNHRQRMEERVHCTACG"
     gene            1020..2516
                     /locus_tag="VFV87_19550"
     CDS             1020..2516
                     /locus_tag="VFV87_19550"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5106028.1"
                     /translation="MSAPLDCDVAVIGAGFAGSLLALIARRLGLRVVLLERDRHPRFA
                     IGESSTPLANLLLEELADRYELPELLPFVKWGAWQRDRPEISCGLKRGFSFFHHTEGQ
                     FWSPNAARSNELLVAASPHDAIADTHWYRADVDHAFAKLAMQRGAELFEQTALQSIEW
                     AGSGAEISASVQGTPMKFRCRFVLDASGPRGFLSRRLSLREAQFPAMPPTQTLFSHFR
                     DVTRWDQVHRPTETPPYPVDDAALHHVFDGGWIWVLRFNNGITSAGVAGTDALFHRLG
                     LAEGASGWDRLLSRLPSVREQFRQARSCLPFRHWPRLTFRVARAANERWALAPGAAAF
                     VDPLLSSGFPLTLLGIQRLAELLERDWRTERWRANLAAYEAATFQEVDWTADLVGALY
                     QNMGRFETFSNLSLLYFAAAIFSESARRLDRKESAPGFLLCRHKAFSAAFRRCLEAAR
                     HESPAGEFSAGILKAIEDVNVAGLGDPSRRNWYPVRAEDLVSVAAKLA"
     gene            2712..>3587
                     /locus_tag="VFV87_19555"
     CDS             2712..>3587
                     /locus_tag="VFV87_19555"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5106029.1"
                     /translation="MKTSLAWLTCLCSAVTLLGAEADTPAPPTSTLSSAPRKVCIVPV
                     RDEIMPPIVYTIRRGVKEAIDKKADLLVLNMDTPGGDVGVTRQIIEILEQFKGDTVTF
                     VNKDAFSAGAFIAVATKKIYMAPSSVIGAAAPWVPTPGGDGPAAVPDTYEKKMTSAVR
                     AMVRATAEKNGYNLAVIEAMIDKSRGLTIDGTVIAKEGEILTLTNTEAEKEYGNPPTK
                     LLSSGTALNLDQLLSKLGYGSATRIVIEPTGLEKLGSWLNTLSPLLLMVGMIGLYLEF
                     KTPGASVFGIVGVCAF"
BASE COUNT          655 a         1135 c         1066 g          731 t
ORIGIN      
        1 caaaacagca gtcgatgagc attgagcaat cgagccatgt cgctccttac ccggactccc
       61 ccgctgctcg cgatcgctgg attctcgatc gccgccccgc ccgccttccc gtcaacccgg
      121 cccagcctaa cgcctgcttc atcgaacagg agcgggacgt ctccggcgaa atcgtccccg
      181 tcgccaccct ctttctcacc aatcgcgaat gcccctggcg ctgcctcatg tgcgatttat
      241 ggaagcacac gctcaccgtg tccgtcgcgc ccgggagcat ccctcaacag attgatcacg
      301 cgctgcaggc cttgacggcg catgcggcgg ctgccccacg acaaatcaag ctctacaaca
      361 gtggcagctt tttcgatgcg cgcgccattc cacccgagga ccatccggcc atcgcggacc
      421 gagtccgcgg cttcgaacgc gtcatcgtgg aatgccaccc ggcgttgatc agcgaacgca
      481 tcctgccctt tcgggatcgc atcgggaccc agctggaaat cgccctggga ctggaaacgg
      541 tgcacccgga gatcctgccg cgactcaaca agcgcatgac tgcggcgcaa tttgcggagg
      601 ccgccggctt tctgaaaacg catggcatcg ccctccgtgt cttcgtcctg gtcaagccac
      661 ccttccttgc cgagtcggat gcctgcttgt gggccggacg ttccatcgac tttgcctatg
      721 attgcgatgc cgccgttgtt tcactgatcc ctacgcgatt gggaaatggc gctctcgaag
      781 cgctggccgc gacgaacgaa ttttctccgc cgcgtctgga tacatttgaa gccgctgtcg
      841 actacggaat gggtctgcag cgcgggcgcc tctttgccga cctttgggat ctccagcagt
      901 tcgcaacctg cgcgacgtgt cttcccgaca gacaggagcg cttgaatcgc atgaatcatc
      961 ggcaacgcat ggaagaacga gtgcattgca cggcctgcgg ataggagggt tctgttctca
     1021 tgagcgcgcc tttggattgc gatgtcgccg tgatcggtgc gggtttcgcc gggtcgttgc
     1081 tggcgttgat tgcccgccgt ctcggattgc gcgtcgtgct gctcgaacgc gatcgacatc
     1141 cgcgtttcgc gatcggcgag tcatccacgc cgctcgccaa tctgcttttg gaggagctcg
     1201 ctgatcgtta cgaacttccc gagctgcttc cctttgtcaa atggggcgcg tggcagcggg
     1261 atcgacccga gattagctgc ggcctgaaac gaggattcag cttctttcat cacaccgaag
     1321 gccagttctg gtcgcccaat gccgcacgtt cgaacgaact gctcgtcgcc gccagtccgc
     1381 atgacgccat tgccgacacc cattggtatc gcgccgacgt ggatcacgcc ttcgcaaaac
     1441 tcgccatgca acgcggcgcc gaactattcg aacaaacagc cctgcaatcc atcgagtggg
     1501 ccggttcggg cgccgagatt tccgcatccg ttcaaggcac gccgatgaag ttccgctgcc
     1561 ggtttgtcct cgatgccagc ggtccacggg gatttctcag ccgcaggctg tcccttcgcg
     1621 aggcacaatt cccggccatg ccacccacgc aaaccctctt ctcgcacttc cgcgacgtga
     1681 cgcgttggga tcaggtgcat cgccccacgg aaacgccgcc ctaccccgtc gatgacgcag
     1741 ccttgcacca tgtcttcgac gggggatgga tttgggttct gcgtttcaac aacggcatta
     1801 ccagcgccgg ggtggccgga accgatgcgc tcttccatcg actgggattg gccgagggag
     1861 cgagcggttg ggatcgcctg ctcagtcgac tgccgtcggt gcgggaacaa ttccgccagg
     1921 ctcggagctg ccttccattt cgtcattggc ctcgactcac gttccgggtg gcccgggcag
     1981 cgaatgagcg ctgggccctg gcgccggggg cggcggcgtt tgtcgatccg ctgctttcgt
     2041 cgggattccc cctgacgctc ttaggaattc agcggcttgc ggaactgctc gagcgcgatt
     2101 ggcgaacgga acgttggcga gcgaaccttg ccgcctacga agccgccacg tttcaagaag
     2161 tcgattggac ggccgactta gttggcgcgc tctatcagaa catggggcgg ttcgagacct
     2221 tctcgaatct gagcctgctt tacttcgctg cggcgatctt tagcgagtcc gcccggcggt
     2281 tggatcgaaa ggagtcagcc cctgggtttc tgttgtgtcg gcacaaagca ttcagcgcgg
     2341 cgtttcgtcg gtgtttggaa gcggccagac atgaatcgcc agcaggcgag ttttctgccg
     2401 ggattctgaa agcgatcgaa gacgtgaatg tcgcgggttt aggggaccct tcgcgacgca
     2461 attggtatcc cgtgcgggca gaggatttgg tttctgttgc ggccaagctt gcgtagcgca
     2521 gggcagtcac acttaccgtc ggatcatacg cgatggccca ggagagtgtg ggcggatgcg
     2581 tgagcgatgg agaactcggg cgtcgctgcg gctcaaagag ccgcggcccg ggcctttggt
     2641 ttgagccgcg cagagcgggc gcacaccgac cttgccttgc gatggctctt cgcgtgcgat
     2701 agcctcggct tatgaaaacg agtctggcct ggctgacctg cctctgcagc gccgtgacat
     2761 tactcggtgc cgaggccgac acgcccgctc cgcccacatc aaccctttca tccgctccca
     2821 ggaaggtgtg catcgtgccg gtgcgcgacg aaatcatgcc tcccatcgtc tacaccatcc
     2881 gtcgcggcgt gaaggaagcc atcgacaaaa aggccgacct cctcgtgctc aatatggaca
     2941 cgccaggggg cgacgtcggg gtcacccggc agatcatcga gatcctggag cagttcaagg
     3001 gagataccgt caccttcgtg aacaaggacg cgttttccgc aggggccttc atcgctgtcg
     3061 ccaccaagaa gatttacatg gcccccagca gtgtcatcgg tgccgccgca ccttgggttc
     3121 caacgcccgg tggtgacggt ccggctgcgg tccccgacac ctatgagaag aagatgacct
     3181 cggctgttcg ggccatggtg cgggccaccg cagaaaagaa cggctataat ctggcggtga
     3241 ttgaagcgat gatcgacaag agtcggggac tcaccatcga tggcacggtc atcgccaagg
     3301 aaggtgagat tctcaccctg acgaacacag aggctgaaaa ggaatacggg aaccccccga
     3361 cgaaattgct ctcctcaggg acggccctca acctcgacca gttgctctcc aaacttggct
     3421 atggcagcgc aacccgcatt gtcatcgaac caaccggact cgagaagctc ggctcctggt
     3481 tgaacaccct tagccctctg ctgctcatgg ttgggatgat cgggctttac ctcgaattca
     3541 aaacgcccgg cgccagcgtg tttgggatcg tcggcgtctg cgcgttc
//
LOCUS       DASUGB010001033         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_255424, whole genome shotgun sequence.
ACCESSION   DASUGB010001033 DASUGB010000000
VERSION     DASUGB010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255424"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..941
                     /locus_tag="VFV87_19560"
     CDS             102..941
                     /locus_tag="VFV87_19560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5106030.1"
                     /translation="MLFSLLADRFVLRPSRDQIDIPHKQRREVSCAGMRVEVWTERSP
                     AASAPEELREPELFVLKFNGMGGRAERTTVHPLEVWPDLPGEVWSANPPGYGGSEGRA
                     SLKSLAAVARSVFEELLAVAGSRPIIVAGNSLGTTSALHVAAHFANVPNLVGLVLRNP
                     PPLPELIRGRYAWRTFGLAHGVAKRIPPELDSVANAARVRLPCVIQQAGRDRIVPPRY
                     QQLIVEAYAGPKQVVTVPAADHAFVLAPEDAGPYVQALSWLRSHSGLAEPNPFPLDSP
                     AIS"
     gene            complement(964..2520)
                     /locus_tag="VFV87_19565"
     CDS             complement(964..2520)
                     /locus_tag="VFV87_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106031.1"
                     /translation="MLATVGVTKWAAVDFSETVLNSQAMQIGGYGGDLSQPESETNLH
                     PRTVSSFQSLFNSSRPWLDMNGDGVVWGPVDSELAIDAIMKKVEQDFSPYDLKAFRGD
                     FAAYRWILTDGIVGDGIVLVTGDSSFIPDERGGIQHLFGHSPPADRELRRNDHDEIVF
                     VFGKSIAESAYRDFAPSGNQAVRDAFITGVASTISHELGHTFGLWHNQPSATQSDLER
                     QALVNDMMSAVSLGHDQAFLDIAYLITPSLSPQNSHRHLLRGDILGPSKNSWAAVLRP
                     GELTISGNASSNDIGVWEFGGSISGYSIQIDGRSIIAEHRATSAYSLNLFDQVLTTIL
                     VYGNDGADKITIHPSVQITTKLYGGNGADRLYGGSGRDYLYGGEGADYLYGLAGSDYL
                     FGQDGNDWLYGGSEADYLYGGADNDHLYGDAGADYLYGDSGDDFLDGGRDSATDRLFG
                     GSGRDTLVQNYYQWWHYSNVIITPDSPPPQRVMDWVAEDSLYDYQANVDWLYGLYWDD
                     TGRLVMTSLR"
     gene            complement(3636..4529)
                     /locus_tag="VFV87_19570"
     CDS             complement(3636..4529)
                     /locus_tag="VFV87_19570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5106032.1"
                     /translation="MGYQDRDYYREGSAQTQFVTSVVVKLIILNALVFLADVFFGGQE
                     HEITRTLALDADSLQKPFYWWQFFTAGFTHGDIGHIFFNMLGLYVFGKPLEERYGGRE
                     FLRFYMIALVTGMVLWAVREYVWSVDVAGAQPVRHILLGASGAVTAVTILFCLLYPRA
                     TLLAAFLFPVPAWLVGAIIIAVNVLGLRQASSIEGDHVAYDVHLFGAAFALAYWYFGW
                     NFGQLPGFAELRRMVASPKKWLQPRPALKVHDPEQYYEDLDTEADRILDKLHREGEAS
                     LTPQEKRTLEDYSRRMRQKLR"
     gene            4911..5372
                     /locus_tag="VFV87_19575"
     CDS             4911..5372
                     /locus_tag="VFV87_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106033.1"
                     /translation="MSGLGKVLNSYGGKSLSMVLGIAGGMAGLGLVALIVGAMMREYF
                     MPAVAVSVLMLIIAIMYVATNLVGVFTKAEVCQGGIRITGLKGTKEIAWDEIDHIAVG
                     RHRLNDKLRWAVTIYHASGEQTDLSPEFWDSAGGTTTFIATVRPFTSVEMV"
BASE COUNT         1029 a         1630 c         1681 g         1054 t
ORIGIN      
        1 gtctggtgtg ggctaggatt gattgcggct gcggaagccc gcggctcaga tcgtgcgtag
       61 aatggagtgt ctccagcctc cggcccgccc cactgcccga aatgctcttt tcgctcctcg
      121 ccgaccgctt cgtccttcgt ccgtcgcggg atcagatcga cattccgcac aagcagcggc
      181 gcgaagtgtc gtgtgccggg atgcgggttg aggtttggac cgagcgctct ccagccgcaa
      241 gcgcgccaga agagctccgc gagccggagc tcttcgtcct caagttcaat ggcatggggg
      301 gccgggctga gcggacgacc gtccatccgc tagaggtctg gcccgatctg ccgggcgaag
      361 tctggtcggc taatccgccc ggctatggcg gcagcgaggg ccgggcaagc ctcaagtcgt
      421 tggccgcggt cgcccgcagc gtctttgaag agctgctcgc cgtcgccggg agccggccga
      481 tcatcgtcgc gggcaacagc ctgggaacga cttcggccct gcacgtggcg gctcatttcg
      541 ccaatgtgcc gaacctcgtc ggcctggtgc tccgcaatcc gccgccgctg ccggagctga
      601 ttcgcggccg gtatgcctgg cggaccttcg gactggccca cggcgtggca aaacgcatcc
      661 cgccggagct cgacagcgtc gccaacgccg cccgcgtgcg gctgccgtgc gtgatccagc
      721 aggcgggccg cgaccggatc gttccgccgc gataccagca gctcatcgtc gaggcctacg
      781 ccggtcccaa gcaggtcgtc accgttcccg ctgccgacca cgccttcgtc ctcgccccgg
      841 aagacgccgg cccctacgtc caagccctga gctggctgcg atcccattcc ggtctggcag
      901 agccgaaccc gtttccgctg gattcgccgg ctatctcgtg aggcgtttcg cctctgcgtc
      961 ggatcagcga agggatgtca tgaccagccg gccggtgtcg tcccagtaca ggccgtacaa
     1021 ccagtccacg ttcgcctggt agtcgtagag cgaatcctcg gcgacccaat ccatgacgcg
     1081 ctggggcgga ggactgtcag gcgtaataat gacgttcgaa tagtgccacc actggtagta
     1141 gttctgcacc agcgtgtcgc ggcccgagcc gccaaacagc cgatcggtgg ccgaatcgcg
     1201 cccaccatcg aggaagtcgt cgccgctgtc gccgtagaga taatcggccc ccgcgtcgcc
     1261 gtacaagtgg tcattatcgg cgccgccgta gagatagtcc gcttcggaac cgccatagag
     1321 ccagtcgtta ccgtcctggc cgaacaggta atcgctgcca gccaggccgt atagatagtc
     1381 ggcgccttct ccgccgtaga gataatcgcg tccgctgccg ccgtagagtc gatcggcacc
     1441 gttgccgcca tagagcttcg tggtgatctg aacggacggg tggatcgtga tcttgtccgc
     1501 gccgtcgttg ccatacacca ggatggtcgt caggacttga tcgaacaggt tgagcgagta
     1561 cgcgctcgtt gcccgatgtt cggcaatgat cgatcggcca tcgatctgga tggaataccc
     1621 cgagatcgat cctccaaact cccagactcc gatgtcgttc gaactggcat tcccggaaat
     1681 cgtcaattct cccggccgca acaccgccgc ccaactgttc ttggaagggc cgaggatgtc
     1741 gccgcggagc aagtgacgat gcgaattctg cgggcttagc gagggtgtaa tcaaataggc
     1801 aatatccaag aatgcctgat cgtgaccaag gcttacagct gacatcatgt cgttcactag
     1861 cgcttggcgc tccagatcgg attgggtcgc gctgggctgg ttatgccaca agccaaacgt
     1921 gtgtcccaat tcgtgcgaga ttgtggatgc aacacccgtg atgaatgcat ctcgcacggc
     1981 ctggttacca gacggggcga aatctcgata cgcactttcg gcgatcgact tgccaaagac
     2041 gaaaacgatc tcgtcatggt cattgcgccg cagttcgcgg tcggcgggcg gtgagtgacc
     2101 aaacaaatgt tgaataccgc cacgctcgtc tgggataaat gaagagtctc ccgtaacgag
     2161 gacgatgccg tcgccgacaa tgccatctgt aagaatccag cgataggcgg caaaatcacc
     2221 cctgaacgcc ttaagatcgt aggggctgaa atcctgttct accttcttca tgatcgcgtc
     2281 gatcgccaat tcgctatcga ccggacccca gacgacgcca tctccgttca tatccagcca
     2341 gggacgcgag ctgttgaaca ggctctgaaa actgctgacc gtgcgagggt gcaagttggt
     2401 ttcactttcc ggctgggaca gatcgccgcc ataccctccg atctgcatgg cctggctgtt
     2461 gaggaccgtt tcgctgaaat ccacggcggc ccacttggtc actcccaccg tggccagcaa
     2521 ctctcggcct tccagccgct cgaattgcgg gcgtcgccct tggcgctcgg tgcgccgccg
     2581 ctggctcgat cctttcacga cttgaacgat ttggttgagc caattcatgg ttcgtcaact
     2641 cctgacgttg ggagagtgcg gcgttcttgc tccgccattt tcgccgtcac ggaagcgacg
     2701 ctcgctagct cgtcgatgag tgaacctgca ctgattggct atggccattc atgccgtcga
     2761 accgacactg ccgttcaaga aagaagcgag tatggaaagg cggaatccgg tggatggacc
     2821 ggcggccctt tccgccgctt tcgggggctg gactactcca ggtagtaggt cagggatctt
     2881 ttgaccacaa gagattgtgc tactattgac atacatgaac aaatatgtac aattcggtct
     2941 ggccgaacga cggccgactg cactgtcggt gcgcgacgtc gctctttcac aagctggtgg
     3001 tgagttggga gcgcaggcgt ctcgcttcgc gcgcgagggg tgggtcatgg gtcaggagcg
     3061 gggtcagggt caggagccag ttggggcgta ccgatgcccg ctcagctccg cccacttacc
     3121 cacaacggcc ctctcccaga cggagaggag gaaaagatgg aggaaaaata gttggcaacg
     3181 cgcaacagcg caacatcctc gaacgggggg cgttgcacgg cgcaacttgt tgtcggaaag
     3241 cgagttgcga aaatgttgcg cggatggctg ggtcgcgaaa aaagttcgga aagttgcgcg
     3301 aggtggtcga atcggctcgg cgcgagcagc gattacggac gggaagtcac aggcgactcg
     3361 cggcgtgcgg cgatccttga tgcagggggc aatacggttt atttattgac gtaacccccg
     3421 tgattcgccg tttttgtcag tttaatcgcg agaggcaggg gcaaaagacg cggtgaatcg
     3481 aagcgaaatg cgattcagac aggtcgaatt gattgacgta aggggtctga acttgtcaac
     3541 caatgcacgg aaaagtttag cgctgatggt agctgtgaac accctcgctg acgcatcggt
     3601 gaggctgcct cgcttacgca tcgggttagt ggcccttacc gcagcttctg gcgcatgcgg
     3661 cggctgtagt cttcgagcgt ccgcttttcc tgcggcgtaa ggctcgcttc gccctcgcgg
     3721 tggagcttat cgaggatccg gtcggcttcg gtgtcgagat cctcgtagta ctgctcggga
     3781 tcgtggactt tgagcgccgg ccgcggctgg agccattttt tgggcgaggc gaccatccgg
     3841 cgcagttccg caaaacccgg caactgcccg aaattccagc cgaagtacca gtaggccagg
     3901 gcgaacgcag cgccaaagag gtgaacatcg taagcgacgt ggtctccttc gatcgacgag
     3961 gcctgccgca gcccgagcac gttgacggcg atgatgatcg cccccaccag ccaggccggc
     4021 accggaaaca ggaacgcggc cagcagcgtg gctcgcggat agagcaggca gaagaggatc
     4081 gtcaccgccg tgaccgcccc cgaagcaccc agcaggatgt gccgcacggg ctgggcgcct
     4141 gcgacgtcaa cgctccagac gtattcccgc accgcccaca ggaccatgcc cgtgaccagc
     4201 gcgatcatat aaaaccgcag aaattcacgg ccgccgtagc gctcctcgag tggtttgccg
     4261 aagacataca ggccgagcat gttgaagaag atgtggccga tgtcgccgtg cgtgaagccg
     4321 gcggtgaaga actgccacca atagaacggc ttttggaggc tgtcggcgtc gagggccagc
     4381 gtccgcgtga tctcgtgttc ctgcccgccg aagaagacat cggccagaaa gacgagcgcg
     4441 ttgaggatga tcagcttgac caccaccgag gtgacgaact gcgtttgcgc gctgccttcg
     4501 cggtagtaat cccgatcctg atatcccata tcctgcctct tgccgctcct gcccactccg
     4561 ggcgtaggac ggagctgtgc tccgtcctgt acgcagccgt ctcctgccct tgtcgagcat
     4621 tcagataatc ttacgacgcg gacggggacg tggcgagtgt gcccctggct ggagccccgg
     4681 ccttagccgg gtgcgggata ccgtcccctc cggctgcgaa ccataattgc gactttacaa
     4741 gacgacgggg gctgattcat ggcaattcgc acgatctggc gtatgtcgcc ggctgccatt
     4801 tcgctgtcgc cggcacgtgc cgcgcggccg agtctcgccc tcttcgtgac cgacgatcag
     4861 ggccaggatt cgtgcgggtg ctacggcaat ccggtaggag gagcaagcca atgagcggct
     4921 tgggcaaggt cttgaattcg tacggcggca aatcgctctc gatggtgctg gggattgccg
     4981 gcggaatggc ggggcttggc ctggtggcgc tgatcgtcgg ggcgatgatg cgcgaatact
     5041 tcatgccggc tgttgccgtt agtgtgctga tgctgatcat cgcaatcatg tacgtcgcga
     5101 cgaacctggt cggcgttttc acgaaggccg aggtgtgcca gggaggcatc cgcatcaccg
     5161 gccttaaagg gaccaaggaa atcgcctggg acgagatcga tcacatcgct gttggccggc
     5221 atcgcctcaa cgacaagctt cgctgggccg ttacgatcta ccacgccagc ggcgaacaaa
     5281 ccgatctctc gcccgagttc tgggattccg ccggcggcac aaccacgttc atcgccacgg
     5341 tccggccgtt cacgagcgtc gagatggttt agccggctgg tctttcaagc gtcc
//
LOCUS       DASUGB010001034         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1365323, whole genome shotgun sequence.
ACCESSION   DASUGB010001034 DASUGB010000000
VERSION     DASUGB010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365323"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFV87_19580"
     CDS             <1..553
                     /locus_tag="VFV87_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106034.1"
                     /translation="YCLSFSIVFASLVASSLPAAEPLQSGLQVGERLTNIFEPLNVTG
                     PYAGEQHCLVCEMGLSPVAMVFAREVNEPLVKLLAQLDKATEKNRDVELGSFVVFLSE
                     KEGLKEQLENVAKKQGLNHIILSTYDPAGPEGFKVAKDSDVTVVLYRQHLVKANHAIR
                     KGELNEQAVEKIVGDLPKIVGEK"
     gene            624..1913
                     /locus_tag="VFV87_19585"
     CDS             624..1913
                     /locus_tag="VFV87_19585"
                     /inference="COORDINATES: protein motif:HMM:NF041199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trx7/PDZ domain-containing (seleno)protein"
                     /protein_id="HEX5106035.1"
                     /translation="MGRTLLVVLFLLLAIDRPAPAGERTPLGKSLNDIETADHWIYDD
                     WPAAVAEAKKTGKPILAVIRCVPCPPGKDLDLKVMQPNKDLAELEQQFVCVRIIQTNR
                     LDQKIFQYDYDMSWSCMFLNANGTVYGRYGSRSASGPGSDSILNAGAFSKAARRALEL
                     HKGYPANAKQLAAKTGPDPEYAVPTQIPGLTEKPASASVRQECIHCHMVKEFALRAKW
                     EEGRLKAEDLFVYPMPQRIGLTMSQEDDLKVQRVEEGSAADEAGIEAGDTLVSLGGQP
                     LVSMADIQWVLTHTPGEAKLPVKFTRGGQTEESKLELSGDWKKSDIAWRASSWYALRQ
                     GVKFEPLPAADRKARGIGEDELALVVKGLFGKGGPKVQQAGLRQNDVLVAVDGKTQAM
                     TESEFLVDLRLQHGPKDSVKFTVLRGDQRRDFDVPLW"
     gene            2075..>4099
                     /locus_tag="VFV87_19590"
     CDS             2075..>4099
                     /locus_tag="VFV87_19590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5106036.1"
                     /translation="MRTLATTVCLLLVSTATAADPFEANKGDHVSLIGNTLADRMQHH
                     GWLETLLVSRFADRDLVFRNLGFSGDEVTTRLRSANFGEPDQWLTRSEADVIFAFFGY
                     NESFAGEAGLARFKQDLDAWIKHTLSQKYNGESAPRLVLFSPIAQEDTGDHNLASPEA
                     NNGRLKLYTAAMAEVAAANSIAFVDLFTPTSAGYASDAKNWTFNGIHLTNYGDQRLAS
                     VIERALTPSGPLFKRDAAQLERLRAAINEKNAQWHARYRTVDGYNVYGGRSQEQYESG
                     VGGPKIKNFVVMQEEMTQRDVMTANRDKRVWAVARGGDLTVSDSNLPPVTQIGPNREN
                     LSPYLGAEEAIQRMTLATGCKINLFASEEQFPELVNPVQMAWDTKGRLWVAAWPSYPE
                     MRPTDEVGDKLLVFEDTNGDGQADTCTPFLDDLNCPTGFQFFQDGVLVMQAPNLWFVR
                     DTDGDGRGDSKELVLMGIDSADSHHTTNAMVLDPGGATYLSDGVFHRTQVETPWGPVV
                     RNNDGCIFRYEPLTQKFERYVSYGFANPHGRVFDRWGNDIITDATGNANYFAPAFSGF
                     VEYPGKHPGMNQFWDRPSRPCPGTGLVSSRHFPEDWQGNFLNCNVIGFQGIFRVKVTE
                     AGSGLKGETLEDLVKSSDPNFRPSAVSCAPDGSIYFLDWQQQLIGHLQHHI"
BASE COUNT          826 a         1266 c         1290 g          717 t
ORIGIN      
        1 atactgcctt tctttttcga ttgttttcgc atctctcgtc gcgagttcgc tgcccgcggc
       61 cgagccgctc cagtcggggc tgcaggtggg cgagcggctg acgaatatct ttgagccgtt
      121 gaacgtgacg gggccgtatg cgggcgagca gcattgcctg gtgtgcgaga tggggctgag
      181 tcccgtggcg atggttttcg cccgcgaggt gaatgaaccg ctggtgaagt tgctggcgca
      241 gctcgacaaa gccaccgaga agaaccgcga cgtagaactg ggcagttttg tggtcttcct
      301 cagcgagaag gaagggctga aggagcagct tgaaaacgtc gcgaagaaac aggggctgaa
      361 ccatattatc ctcagcacgt acgacccagc cggtccggag ggcttcaagg tcgccaagga
      421 ttccgacgtg acggtcgtcc tctatcgcca gcacctggtg aaggccaatc acgccattcg
      481 caagggcgag ttgaacgagc aggccgtcga gaagatcgtc ggcgatctgc cgaaaattgt
      541 cggtgaaaaa taagtcttag cgagccgtaa agagccgtag gatggacctc caggtccgtc
      601 catctttgac cgtggagaat cacatgggcc gcaccttgct tgtcgtattg tttcttctcc
      661 tcgcgattga ccggcctgcg ccggccggcg agcgaacgcc gctcggcaaa tcgctgaatg
      721 acatcgaaac ggctgaccac tggatctacg acgattggcc ggcggcggtc gccgaggcga
      781 aaaagaccgg caagccgatc ctggctgtca ttcgctgcgt tccctgcccg ccggggaagg
      841 atctcgacct gaaggtcatg cagccgaaca aggacttggc cgagttggag cagcaatttg
      901 tctgtgtccg catcatccag acgaaccgct tagaccagaa gatctttcaa tacgactacg
      961 acatgtcgtg gtcctgcatg ttcctgaatg ccaacggcac ggtttatggc cgctatggct
     1021 cgcgaagtgc ctcggggccg ggctccgact cgatcttgaa cgccggggca ttttccaagg
     1081 ccgctcggcg tgccctggaa ctgcacaagg gctatccggc gaatgcgaag cagcttgccg
     1141 ccaaaacagg accggacccg gaatacgccg tgccgacgca gattcccgga ctcaccgaga
     1201 agccggcctc ggccagcgtg cggcaggagt gcatccactg ccacatggtc aaggaattcg
     1261 ccctgcgggc caagtgggag gaaggacgcc tgaaggcgga agatctgttc gtctatccga
     1321 tgccgcagcg gatcggcctg acgatgagtc aggaagacga cctaaaagtc cagcgcgtcg
     1381 aggaagggtc ggccgcggac gaagcgggca tcgaagccgg cgatacgctc gtctcgctcg
     1441 gcggccagcc gctcgtctca atggccgata tccagtgggt gctcacgcac acgcccggag
     1501 aagcgaaact gcccgttaaa ttcacgcgcg ggggccagac ggaagagagt aagctcgaac
     1561 tgagtggcga ctggaagaaa tccgacatcg cctggcgggc ctcgtcgtgg tatgcgctgc
     1621 ggcagggagt gaagttcgag cccttaccgg cagcagacag gaaggcccgc ggaatcggcg
     1681 aggatgaatt ggcgctggtc gtcaaaggcc tgttcggcaa aggcgggccg aaagtccagc
     1741 aggcgggcct gcgccagaac gacgtccttg tcgccgtgga cggcaagacc caggccatga
     1801 ccgaatcgga gtttctggtc gatctgcggc tccagcacgg gccgaaggat tcggtcaaat
     1861 tcaccgtcct ccggggcgac cagcggcgag atttcgacgt gccgctgtgg tagagtgggg
     1921 agtttgctaa cccatcccac ctggttccca agctcctgct tgggaaccca cgcccgggaa
     1981 gctcccgctt cccgtccgcg aagcagagct tcggcaacat gcgtacccaa gcagagcttg
     2041 ggcacgagta cggccagttc cctggagcct cggcatgaga actctcgcga ccaccgtctg
     2101 cctcctctta gtcagcactg ccaccgctgc cgacccgttc gaggcgaaca agggcgatca
     2161 tgtttccctt atcggcaaca cgctcgccga ccggatgcag catcacgggt ggctggagac
     2221 gctgctggtc agccgctttg ccgaccgcga tctcgtcttc cgcaatctgg gcttttcggg
     2281 cgatgaggtc acgacccgcc tgcgcagcgc caactttggc gagccagacc agtggctcac
     2341 caggagcgaa gccgacgtga tttttgcctt cttcggctac aacgaatcgt ttgccggcga
     2401 agcggggctg gcgaggttca agcaggatct ggacgcctgg atcaagcaca cgctcagcca
     2461 gaaatacaac ggcgaaagcg ccccgcggct ggtgctcttt tcgcccatcg cccaggaaga
     2521 cacgggggac cacaatctgg cgagccccga ggccaacaat ggccgcctca agctctacac
     2581 ggctgccatg gccgaagtcg ccgcggccaa cagtatcgcg tttgttgatc tcttcacgcc
     2641 gacgagcgca ggctacgcca gcgacgcgaa gaattggacc tttaatggca tccatctgac
     2701 caattacggc gaccagcggc tggcgtcggt catcgaacgg gcactgacgc ccagcgggcc
     2761 gctcttcaag cgcgatgcgg cgcagctcga acgactgcgg gccgcgatca acgagaagaa
     2821 tgcccagtgg cacgctcgct atcgcacggt cgatggctac aacgtctatg gcggccgctc
     2881 gcaggagcag tacgagtcgg gcgtcggcgg gccgaagatc aagaacttcg tcgtgatgca
     2941 ggaggaaatg acccagcgcg acgtgatgac cgccaaccgc gacaagcggg tctgggccgt
     3001 cgctcggggc ggcgacctga ccgtcagcga ctcgaacctg ccgcccgtca cgcagatcgg
     3061 ccccaaccgc gagaacctct cgccctatct cggcgccgaa gaagcgatcc agcgcatgac
     3121 gctcgccacc ggctgcaaga tcaacctgtt cgccagcgaa gagcagtttc ccgagctggt
     3181 gaaccccgtg cagatggcct gggacaccaa gggccgtttg tgggtcgccg cctggccgag
     3241 ctatcccgaa atgcggccga ccgacgaagt cggcgacaag ctgctcgtgt ttgaagacac
     3301 caacggcgat ggccaggccg atacctgcac gccatttctc gacgacctca actgccccac
     3361 cggctttcag tttttccagg atggcgtgct ggtgatgcag gcgccgaatc tctggtttgt
     3421 gcgcgacacc gacggcgacg gccgcggcga cagcaaggag ctggtgctga tgggcatcga
     3481 ctcggctgat tcgcaccaca cgaccaatgc gatggtcctc gatccgggcg gggcgaccta
     3541 tctctccgac ggcgtcttcc accgcacgca ggtcgaaacg ccctggggac cggtcgtgcg
     3601 gaacaacgac ggctgcatct tccgctatga gccgctgacg cagaagttcg agcggtacgt
     3661 ctcctacggt ttcgcgaacc cgcacggccg cgtcttcgat cgctggggca acgacatcat
     3721 caccgacgcc accggcaacg cgaactactt cgccccggcg ttcagcgggt ttgtcgaata
     3781 tcccggcaaa caccccggga tgaaccagtt ctgggatcga ccctcgcggc cgtgccccgg
     3841 caccggcctg gtcagcagcc ggcattttcc ggaggactgg caggggaact tcctgaattg
     3901 caacgtgatc ggctttcagg ggatttttcg cgtgaaggtg accgaggcgg gctctgggct
     3961 gaaaggcgag acgctggaag acctggtgaa atcgagcgat ccaaatttcc gcccgtcggc
     4021 cgtaagctgt gcgccggatg gctcgatcta cttcctcgac tggcagcagc agctcatcgg
     4081 ccacctgcag caccacatt
//
LOCUS       DASUGB010001035         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1645588, whole genome shotgun sequence.
ACCESSION   DASUGB010001035 DASUGB010000000
VERSION     DASUGB010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645588"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2318
                     /locus_tag="VFV87_19595"
     CDS             <1..2318
                     /locus_tag="VFV87_19595"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5106037.1"
                     /translation="EGGLELKTLGTDLDDQALFAKWERIHDRVLAGEMPPALADVPPP
                     ADRAAFLKSLAEPLAAAHMRRKATVLRRLNRREYENTLNDLFGTNLKLAELLPEDGRS
                     HEFDNVGEALGISLVQMQRYMESAEAVLDAAIARTVEPPPSKVVRASYADTRGAEQWL
                     NKIWLHRDDGAVVFFKQYGYPSGMLREANVQREGWYTVRVTGYAFQSDEPITFSVGGT
                     TFARGLEQPTFGYFSLPPGEPTTIELRAWISRNYMIDITPYGISDNNNEIRQKGVQGY
                     DGPGLAIQHVEIEGPLVDEFPSRGHKLIFDGLNRREVPPRNPADRRRPNYVAKFEIAA
                     DDPAAAAAPVLARVAERAFRRPVPPERLAPYQTLFEAELKQGASFESALRTAIAALFC
                     SPEFLYLRELPHARGGDSAAGSYWLDDYSLASRLAYFLTRTAPDDELLAAAASGKLST
                     DRDLLLAQAERLLADPRSARFVRDFTDAWLNLRDIEFTNPDAQLFPEFDPFLQWSMVA
                     ETRTYFRKLIDDNLPVTSVVKSDFAILNSRLAEHYGIPGVSGPELRPVKLPAESVRGG
                     FLSQGSVLKVSANGSNTSPVVRGVYVAERILGQAPPPPPPGVPGVEPDIRGATTLREL
                     LDKHRSLDSCKACHQMIDPPGFALESFDPVGGWRDRFRSLGDGERVDLQIRGQRVRYR
                     LGPPVDASGELSGGKEFAGYREFRDLLAAEQQVLARTLATKLLTFATGRELGFSDRAE
                     IERIVSQSAESGHGVKDLIRLAIASEIFRRK"
     gene            2322..3632
                     /locus_tag="VFV87_19600"
     CDS             2322..3632
                     /locus_tag="VFV87_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5106038.1"
                     /translation="MNAPYVSTRGPLSRRHFLRGTGAALISLPLLDAMTPSISRAAEA
                     EQGGSPRRFVAACATLGFHTPFLFPSSQGTDYEPTPYLKPLDKHRREFTVLSGLSHPE
                     QQGNNGHASEMTWLTSAQRPGLAGFRNTISLDQLIAEQIGIETRYPSLVLSTSGRSMS
                     WTSSGVEIPGETSPARLFKALFLDGTEQEVAAELRQLARGRSILDTVLGDARRLEGEL
                     GPRDKNKLDEYLTAVRDLESRLQQSQGWATRPKPKTDASLPQDIQDRNDAIAKQRLMY
                     DMIALALQSDSTRTVTFQLSGMNAVPSNIDGVSSDWHNLSHHGKDPAKIDELRLIEEA
                     EFTAFSDFLTKLKAIEENGRPLLDHTAVLFGSNLGNASSHDWHNLPVIVAGGGYRHGT
                     YVAHDEKQNTPLANLFVSLAQHMGLEIDRFGSSTASSLRGLERT"
     gene            complement(3643..3969)
                     /locus_tag="VFV87_19605"
     CDS             complement(3643..3969)
                     /locus_tag="VFV87_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106039.1"
                     /translation="MSHKNTVRVVTRAADGTLRIRDYPTTEPLLETHTQIGIDDCSTD
                     LALRGMPVFRGLVGPMPEGKHVVRYESPDVFETLTKEWSSARNPRRRRRMQAPETLGA
                     VTEPRE"
     gene            complement(4283..>5266)
                     /locus_tag="VFV87_19610"
     CDS             complement(4283..>5266)
                     /locus_tag="VFV87_19610"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106040.1"
                     /translation="YARVAGANHPRTGMPANILVLPEAAQDGLKLESNFETGALPTLT
                     QPGELGPTYAAFNPAGGGQLKEDLELKIGAERLGDRRQLLASLDQIRRGADSSKPLEG
                     ANRFQQQAFDVITRGVAEAFDLSKEDPRTVERYDTTKLFPARETQQWYDMRRSSNLLG
                     RQMLLARRLCEAGCGFVTVSDCGWDMHANNNSPKFMAGMYPLGGQVDHAVSAFVEDIH
                     ERGLEDKILLVVTGEMGRTPRLNKNGGRDHYGELTPLVFAGGGLKMGQVIGQSDPHAT
                     RAASTPYNPKHLLATIFHSLFDVGKLRLDSSIPHEIIRLAESSQPIDGLVS"
BASE COUNT          901 a         1805 c         1684 g          876 t
ORIGIN      
        1 cggaaggcgg gctcgagctg aagaccctgg gcacggatct ggatgaccag gcgctgttcg
       61 cgaagtggga acggattcac gaccgcgtgc tggccggcga aatgcctccc gcactagccg
      121 atgtgccgcc gcccgccgac cgggccgcct tcctcaagtc gctggccgag ccgctggctg
      181 ccgcccatat gcggcggaag gccacggtgc tgcgccggct caaccggcgc gaatacgaaa
      241 acacgctgaa cgacctgttt ggaacgaatc tcaagctggc cgagctgctt ccggaagatg
      301 gccgctcgca cgagttcgac aacgtcggcg aggcgctggg aatctcgctc gtgcagatgc
      361 agcggtacat ggaatcggcg gaagcggtgc tcgatgcggc catcgccaga acagtcgaac
      421 cgccgcccag caaagtcgtc cgagccagct atgccgatac ccgcggcgcc gagcagtggc
      481 tcaataaaat ctggctccac cgcgacgacg gggccgtcgt gttcttcaag caatacggct
      541 acccgtcggg aatgcttcgc gaggccaacg tgcagcgcga gggctggtac acggtgcggg
      601 tcaccggcta tgcatttcag tccgacgagc cgatcacttt ttccgtcggc gggacgacct
      661 tcgcccgcgg cctcgagcag ccgacgttcg gctacttctc gctgccgccg ggcgagccga
      721 cgacgatcga gctgcgggcc tggatttcgc ggaactacat gatcgacatc acgccctacg
      781 gcatctccga caacaacaac gagatccgcc agaaaggcgt gcagggatat gatgggccgg
      841 ggctggccat tcagcacgtc gagatcgaag ggccgctggt ggacgaattc cccagccgcg
      901 gccacaagct gatcttcgac gggctgaatc gcagggaagt gccgccgcgg aatccggccg
      961 accgccggcg gcccaactat gtggccaaat tcgagattgc cgccgacgac ccggcagccg
     1021 ctgcggcgcc ggtgctggcc cgcgtcgcgg agcgggcgtt tcgacgcccc gtcccgcccg
     1081 agcgactcgc gccctaccag accctcttcg aagccgagct gaagcaaggc gcgtcgttcg
     1141 aatcggccct ccgtacagcc attgcggctt tgttctgctc gccggaattc ctctatctgc
     1201 gggaattgcc ccacgcccgc ggcggcgatt cggccgcggg cagctattgg ctcgacgatt
     1261 actcgctcgc ttcgcgcttg gcttatttcc ttacccgcac agcgcccgat gacgaactgc
     1321 tcgccgccgc tgcaagcgga aaactctcga ccgatcgcga cctgctgctc gcgcaggccg
     1381 agcggctgct ggccgatccg cggtcggccc gcttcgtccg ggacttcacc gacgcctggc
     1441 tcaacctgcg cgatatcgag ttcaccaacc ccgacgccca gctctttccg gagttcgacc
     1501 cgttcttgca gtggtcgatg gtcgccgaga cgcgaacgta ctttcgcaag ctgatcgacg
     1561 acaatctgcc ggtcaccagc gtcgtcaagt cggacttcgc catcctcaac agccgcctgg
     1621 ccgagcacta cggcattccc ggcgtcagcg gcccggagct ccggccggtc aaacttcccg
     1681 cggagagcgt gcggggcggc ttcttgagcc aggggagcgt gctcaaggtc tcggccaacg
     1741 gcagcaacac ctcgccggtc gtgcggggcg tgtatgtggc tgagcggatt ctcgggcaag
     1801 cgccgccgcc tcctcctccc ggcgttccgg gcgtggagcc cgatattcgc ggcgcgacga
     1861 cgctccgcga gctgctcgac aagcatcgca gcctcgattc gtgcaaagcc tgccatcaga
     1921 tgatcgatcc gcccggcttt gcgctggaga gtttcgatcc ggttggcggc tggcgcgacc
     1981 gattccgcag cctgggggac ggggagcgcg tcgatctgca gattcgcggc cagcgggtcc
     2041 gctataggct cggtccgccg gtcgatgcca gcggcgagct atccggcggg aaagagtttg
     2101 ccggctatcg cgagtttcgt gacctgctgg cggctgagca gcaggtgctg gcccgcacgc
     2161 tggccacgaa gctgctcacc ttcgccaccg gccgcgaact cggattctcc gatcgggccg
     2221 aaatcgaacg cattgtgtcg caatcggctg aatccggcca cggcgtgaag gacctgatcc
     2281 gcctggcaat cgccagtgaa atcttccgca ggaaataagc catgaacgca ccgtacgtca
     2341 gcacgcgcgg ccccctgagc cgccggcact tcttgcgtgg cacgggcgcg gcgctgatca
     2401 gcctgccgct actcgacgcg atgacgccga gcatcagccg ggcggcagaa gcagagcagg
     2461 ggggctcgcc ccggcgtttc gtcgccgcgt gcgccacgct cggcttccac acgccgttcc
     2521 tctttcccag cagccaggga accgactacg agccgacacc gtacctgaag ccgctcgaca
     2581 agcatcgccg cgagttcacc gtcctatcgg ggctcagcca tcccgagcag caaggcaaca
     2641 acgggcacgc ctccgagatg acctggctca cctcagctca gcggccaggg ctggccggct
     2701 ttcgcaacac aatctcgctc gaccagttga tcgccgagca gatcggcatc gaaacgcgct
     2761 atccgtcgct ggtcctttcg accagcggcc ggtcgatgtc gtggacctcc agcggcgtgg
     2821 aaatccccgg cgaaacctcg cctgcccgat tgttcaaggc gctgttcctc gatggcaccg
     2881 agcaggaagt ggccgccgag ctgcggcagc tcgcccgggg ccgtagcatt ctggataccg
     2941 tcctgggcga cgcccgccgg ctggaagggg agcttggccc gcgcgacaag aacaagctcg
     3001 atgaatacct gacggcagtg cgcgacctcg aatcgcgcct gcagcaatcg cagggctggg
     3061 ccacgcggcc caagccgaaa accgatgcct cgctgccgca ggatattcag gaccgcaacg
     3121 acgccatcgc caagcagcgg ctgatgtacg acatgattgc gctggccttg cagagcgatt
     3181 cgacgcggac ggtgacgttc cagcttagcg gcatgaacgc cgtgcccagc aacatcgacg
     3241 gcgtgagtag cgactggcac aacctctcac accacggcaa ggacccggcg aagatcgacg
     3301 agctgcgcct gatcgaggaa gcggagttca ccgctttcag cgatttcctg acgaagctca
     3361 aagcgatcga agagaacggc cggccactcc tcgatcacac cgccgtgctg ttcggctcga
     3421 acctgggcaa cgccagcagc cacgattggc acaatctgcc cgtgatcgtc gccggcggcg
     3481 gctatcggca cgggacctac gtcgcccacg acgaaaagca gaacacgccg ctggccaacc
     3541 tgttcgtctc gctcgcccag cacatggggc tggaaatcga ccgcttcggc agcagcaccg
     3601 cctcgtccct ccgcggcctg gagcgaacgt aatcgatcga cgttactcgc gcggttcggt
     3661 cacggccccc aacgtttccg gggcctgcat ccgccgccgg cggcgcggat tgcgggcgct
     3721 cgaccattcc ttcgtcagcg tctcgaagac atcgggcgat tcgtaacgga cgacgtgctt
     3781 gccttcgggc atcggcccga ccagaccgcg gaagaccggc atgccacgga gggccagatc
     3841 ggtcgagcaa tcgtcgatgc cgatctgcgt gtgcgtctct aagagcggct cggtggtcgg
     3901 ataatcccga atccgcagtg tgccgtcggc agcgcgggtc accacccgca cggtgttctt
     3961 gtggctcatg atgggctcct ggttaaaccg cgagccgcat ccttggctac agggttccga
     4021 gttgtagcgg ccgcgggcat cctcgcccgc gcgaaaccgc gagcggcagc cgggccgcat
     4081 ccggcaggca ggccggacag gctgtttatc ggccgcccgc agcccggagg cgcgatggcc
     4141 cgccctgctg ccgcacatgg cgagcaatcc gcgcggccgg catgtcgcgc gcgactgatc
     4201 gggccgctac gagccggcgc accgacaact cggccgctcc gtttgtgccg gttgtgacag
     4261 gcttgcccgt agttggcctg tcttacgaaa ccaatccgtc gatcggctgg ctgctctcgg
     4321 cgaggcggat gatctcgtgc gggatgctgc tgtcgaggcg cagttttccg acgtcgaaca
     4381 gcgaatggaa gatcgtggcg agcaggtgct tcgggttata gggcgtcgac gccgcccgcg
     4441 tggcgtgcgg gtcggactgg ccgatgacct ggcccatctt caatccgccg ccggcgaaga
     4501 cgagcggtgt cagttcgccg taatggtcgc ggccgccgtt cttgttgagc cgcggcgtgc
     4561 gccccatctc gccggtgacc accagcagga tcttgtcctc gaggccccgc tcgtgaatgt
     4621 cctcgacgaa cgcactgacc gcgtgatcga cctgcccgcc gagcggatac atgccggcca
     4681 tgaacttagg gctgttgttg ttcgcgtgca tgtcccagcc gcagtcgctc accgtgacaa
     4741 acccgcagcc cgcctcgcac aagcggcggg ccagcagcat ctgccgtccg agcaaattcg
     4801 aggagcggcg catgtcgtac cactgctgcg tctcgcgggc tggaaagagc ttcgtggtgt
     4861 cgtaccgctc gaccgtccgc gggtcttcct tcgagagatc gaaggcctcg gccacgccgc
     4921 gcgtgatcac atcgaatgcc tgttgctgga agcgattcgc gccctcaagc ggcttggaag
     4981 aatcggcgcc acggcgaatc tgatcgaggc tcgccaagag ctgccgccgg tcccccagtc
     5041 gctccgcgcc gatcttcagc tccagatcct ctttgagctg cccgcccccc gccggattga
     5101 acgcggcata cgtcggcccg agctcgcccg gctgcgtgag cgtcggcagc gcgccggtct
     5161 cgaaattgct ctccagcttc agcccatcct gcgccgcttc cggcaggacg aggatgttcg
     5221 ccggcatgcc ggtccgcgga tggttcgcgc cggccacgcg ggcata
//
LOCUS       DASUGB010001036         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_950036, whole genome shotgun sequence.
ACCESSION   DASUGB010001036 DASUGB010000000
VERSION     DASUGB010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950036"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1512
                     /locus_tag="VFV87_19615"
     CDS             130..1512
                     /locus_tag="VFV87_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5106041.1"
                     /translation="MDRRQFITATSAAGISLAAAAATRQRLIAEEVADQKPKRVGLIG
                     CGWYGKCDLFRLMQVAPVEVVSLCDVDVKQREKAANIVSQRQPQSKEKPRLFGDYREM
                     LKEKDLDICLIGTPDHWHALTMIAACEAGADVYCQKPISVDVREGQAMVAAARKHGRV
                     VQVGTQRRSTPHLVEAREKIVKQGLLGKIGYAEVCCYYHMRANGNPPTQPVPDHLDYE
                     MWTGPAPLRPYDGLPHKRWWRTFMEYGNGIVGDMCIHMLDMIRWQLDLGWPTSVSSTG
                     GILVQKEGKSNTSDTQAATFDFGDLKVVWQHRTWGNPTDNDYPWAGFIYGDKGTLKLS
                     VHRWDFIPQGKQEPTLSGAAVFDPEDKYPEDKTEAELTGDQQEAHVLTANRAHQRDFL
                     AAIASRGKPIADIEQGHISTASCILANLSMQLGRSLTWDPAKHEVVGDADANNLLARP
                     YRKPWVHPQA"
     gene            1523..2401
                     /locus_tag="VFV87_19620"
     CDS             1523..2401
                     /locus_tag="VFV87_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106042.1"
                     /translation="MNAKTFFAQPPKAKRAVLSPYGSATLKGQYGIRIELQPLVFSAP
                     PESASSETDTDIILDHLDIPSIVPKELEHSRYRPNAQSYEGSCYFLGQHNWVDLNEIR
                     FLGASGPRIRAEYDLIIHLPIALPDEYPLVLTVSTKVEPDTHKFVPRQPKYVPSIGTF
                     TETHEHFWECPTTYHGCPVTVQLHAERGQFRRIIAFVKSVLAENAITPTDILKEISER
                     IGELQWKFDHFKVRTRFTPERLAPNWFSFYLDDRSDELTLSIRLSDPADTGQWFLRFH
                     GTEAYDLEWIPHEALS"
BASE COUNT          523 a          814 c          729 g          470 t
ORIGIN      
        1 cgttcggggt tcgggtaagg gatttccacc tatgcggtgt tttccgcacc tgcacgtggc
       61 ttgttggcgc ggcataatgc tcgacgagcc ccaagtccct agccccgaga ccctagcccc
      121 ctctccgcca tggaccgccg ccaattcatc actgctacgt ccgccgccgg aatttcgctc
      181 gccgccgctg ccgcgaccag gcagcgcttg attgctgaag aggtcgccga tcagaagccc
      241 aagcgcgtcg gcctcatcgg ctgcggatgg tatggcaagt gtgacctgtt ccgactgatg
      301 caggtcgcgc cggtcgaggt cgtctcgctg tgcgacgtgg acgtgaagca gcgcgaaaaa
      361 gccgccaaca tcgtcagcca gcggcagccg cagtcgaagg aaaagccgcg gctgtttggc
      421 gactatcgcg agatgctcaa ggagaaggac ctcgacatct gcctgatcgg cacgcccgac
      481 cactggcacg ctctgacgat gatcgccgcc tgcgaagccg gggccgatgt ctattgccag
      541 aagccaatca gcgtcgatgt ccgcgaggga caggcgatgg tcgccgccgc gaggaagcac
      601 ggccgtgttg tgcaagtcgg cacgcagcgc cgcagcacgc cgcacctcgt cgaagcccgt
      661 gagaaaatcg tcaagcaggg cctgctcggc aagatcggct acgcggaggt ctgctgctat
      721 taccacatgc gggccaacgg caatccgccc acgcagccgg tgcctgacca cctcgactac
      781 gaaatgtgga ccggcccggc tccgctgcgg ccctatgacg gcctgccgca caagcgctgg
      841 tggcggacct tcatggaata tggcaacggc atcgtcggcg acatgtgcat ccacatgctc
      901 gacatgatcc gctggcagct cgatctcggc tggccgactt ccgtcagctc gaccggcggc
      961 atccttgtgc agaaggaagg caagtcgaac accagcgaca cgcaagccgc cacgttcgac
     1021 ttcggcgatc tgaaagtcgt ctggcagcac cgcacctggg gcaacccgac cgacaacgac
     1081 tatccctggg ccggcttcat ttacggcgat aagggcacct tgaaactcag cgtccaccgc
     1141 tgggacttca tcccgcaggg gaagcaggag cccacgctca gcggcgccgc ggtcttcgac
     1201 cccgaggaca agtaccccga agacaaaacc gaagccgagc tgaccggcga ccagcaggag
     1261 gcgcacgtcc tgaccgccaa ccgtgcccac cagcgcgact tcctcgccgc catcgccagc
     1321 cgcggcaagc ccatcgccga catcgagcaa ggccacatca gcaccgccag ttgcattttg
     1381 gccaacctct ccatgcaact cggccgtagc cttacgtggg acccggcgaa gcacgaggtt
     1441 gttggcgatg cggacgcgaa taatctgctc gcaaggccct atcgcaagcc gtgggtgcat
     1501 ccgcaagcgt aagtggccct ttatgaatgc caaaacgttc ttcgcacaac ctccgaaggc
     1561 gaaacgggcc gttctttctc cctatggttc cgcgacgctc aagggacaat acggcattcg
     1621 tattgagcta cagccattgg tcttttccgc cccacctgag tcagcgtcca gcgaaactga
     1681 cactgacatc attctggatc accttgacat cccatcaatt gttcccaagg agctggagca
     1741 ctcacgatac aggccaaatg ctcagagcta tgaaggctcg tgttactttc tgggccagca
     1801 caactgggtg gacctgaatg aaatccgttt tcttggagcg agtggtccgc gcatccgcgc
     1861 agagtacgat ctgatcattc atttgccgat cgcactcccc gatgagtatc cgcttgtcct
     1921 gacggtatcc acgaaagtgg agcccgacac gcacaaattc gttcctcgcc agcccaagta
     1981 tgtgcctagt attggcacgt ttacggagac gcatgagcac ttttgggagt gtcccacgac
     2041 ctatcatggg tgtcctgtca cagtccagtt gcacgcagag cgtggccaat tccggaggat
     2101 tatcgccttt gtaaagtcgg tgcttgccga gaatgcaatc acaccaactg acatcctaaa
     2161 agagatcagt gaacgaattg gcgagcttca gtggaagttc gaccatttca aggtccgcac
     2221 tcgctttacg ccggagcgac ttgcgccgaa ctggttctcg ttctatttgg acgaccggtc
     2281 cgatgaactg acattgtcga ttcgcttgag cgatccggca gacacaggcc aatggttcct
     2341 tcgattccac ggcaccgagg cgtacgattt agaatggatc ccgcacgagg cattgagctg
     2401 agcctgcttc agcaggcttg ctcacgtcag gccccggcat gagccggggc tggctcggcc
     2461 gacaagcatc gtgtgccctc agccggcttc cgccggcttc ccagccgcga agcggcggca
     2521 gcgcgtagcc gtgggc
//
LOCUS       DASUGB010001037         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_671157, whole genome shotgun sequence.
ACCESSION   DASUGB010001037 DASUGB010000000
VERSION     DASUGB010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671157"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VFV87_19625"
     CDS             <1..1705
                     /locus_tag="VFV87_19625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5106043.1"
                     /translation="DRVRRQWLVDIARDPPDRHVFTAPVASYEPNSWGLFDLHGNVWE
                     WCEDRYLDTAYAPLAREGYQQIRKRAIDPLNVQPWSSAGDWRVIRGGSWFNSPIQCRS
                     GVRGYFEAGDAACYVGFRVARDASPEQVAAAGEAFEKSEAARETLMRLADRVLERRDG
                     RLTVELSQQRSELAAEFLAALQTVDEPVDLYVDGRGSLTASQLAGLLAARHLRGLAIS
                     SIGPNVAEADFAPLANHPQLELLHLTGAPQLADGIFAHLAKHDRLQELQFDSARITDG
                     GLQSLPPQPRLKSLRISGTASSGLALARFRGAPLETFACERLSDHGAELLGEFSTLRE
                     LWLGGSPITGRGLKAIARLPRLQQLDLSRCSSPGDADFAVLGDLYNLAHLQLNQTAAG
                     DQAAAALVRLNNLHVLQIGSEQLSDSGLRQLCGIVSLRNVRIAPEAANVTDAGLADLW
                     RLVNLDSLALAAPQFTGSGLATLHELPKLQWLSLSGAGIADVACEHAARSESLSQLSL
                     GGWQEGGPQGLTDAGLLHLAGARGLTQLDLFRKGTQVTDAGVAELRSRRPQLKLNAHG
                     P"
     gene            1731..>3279
                     /locus_tag="VFV87_19630"
     CDS             1731..>3279
                     /locus_tag="VFV87_19630"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEX5106044.1"
                     /translation="MSFLRTTWPILLAALGVSAGCRRAEVPPGNRPAAPSVTPVALEN
                     PPPAEVAPQPARPAPPPAPAVDFKTFEDHVTPFLKKHCHKCHGAEQAEAKLRLDTLAA
                     DFLQRPAADHWVEVLDRLNLGEMPPEDEPRPAAEDLAAVTDWITSELRLARERSQSTG
                     GRVLLRRLTRLEYANTVRDLLQVEFAGGQGPLVQLPPDGSLAGFDRVSKALLLDPSLM
                     EAYLSVAQQVADQAIVFRPPLVPERTLRYEFEHTAFNGAISYQLSHRANELDGPFMVV
                     METSARTYGKLRHPYSDKEIPLTGRYRIRVRAAADPGERGEPVFMDVTYGAEGLQGRF
                     RVEATKDAPQIYEFEKTFDAFTPGEFHVGIANGTRFSFGNAEWHERNNELTRLAEEGN
                     MLEATRMKARLRAEGGYDHYVRGSYLPEVLHTETLPKLYLDWIEVIGPLQGEYPPPSM
                     HTVFGDAERAARFVAGDTSREQLLDDARAVFAGLLPRAFRRPVQDAEVERIVGLVAAE
                     LEAGAEPP"
BASE COUNT          536 a         1128 c         1093 g          522 t
ORIGIN      
        1 cgaccgcgtc cgccgccagt ggctggtcga tatcgcccgc gatccgccgg accggcacgt
       61 cttcaccgct cccgtcgcca gctacgagcc caattcctgg ggcctgtttg acttgcacgg
      121 caacgtctgg gaatggtgcg aggaccgcta tctcgatacg gcttacgcgc cgctggcccg
      181 cgagggctac cagcagatcc gcaagcgggc gatcgatccc ctgaacgtgc agccctggtc
      241 gagcgccggc gattggcggg tgattcgcgg cggctcgtgg ttcaattcgc cgatccagtg
      301 ccgcagcggc gtgcgcggct atttcgaagc cggcgatgcg gcctgctacg tgggattccg
      361 cgtggcccgc gacgcttcgc ccgagcaagt tgccgcggcc ggcgaggcgt ttgagaagtc
      421 cgaagccgcg cgggaaactc tcatgcggct ggccgatcgc gtgctggagc gccgcgatgg
      481 ccggctgacg gtggagctga gccaacagcg ctcggaacta gcggccgagt ttctagccgc
      541 cctccaaact gtcgatgaac cagtcgatct gtacgtggat ggccgcggga gtcttacggc
      601 ttcccagctt gccggcttgc tggcagcacg gcatttgcgg ggcctggcga tttcgagcat
      661 cgggccgaac gtcgccgaag cggacttcgc tcccctcgcc aatcacccgc agctggagct
      721 gctgcacctg accggcgcac cacaactggc ggatgggatc ttcgcacatc tggcgaagca
      781 cgaccgtctc caagagctgc agttcgacag cgcccgaatc accgatggcg gcctgcagtc
      841 gctcccgccg cagccgcggc tcaagtcgct gcgcatcagc ggcacggcat cgagcgggct
      901 ggcgttggcg aggttccgcg gagcgccgct ggaaacattc gcgtgcgagc gcctgagcga
      961 ccacggggcc gagttgctgg gtgagttctc cacgctccgc gagctctggc tcggcggcag
     1021 cccgatcaca ggccgcggcc tgaaggcgat cgcccgtctg ccgcgcttgc agcagctcga
     1081 cctgtcgcgc tgctcgtctc ccggcgacgc tgattttgcc gtgctgggcg atctttataa
     1141 cctcgcgcac ctgcagctca accaaactgc agccggcgat caggcggccg ccgccctggt
     1201 ccgcctgaac aacctgcatg tgctgcaaat cggcagcgag cagctcagcg actccggcct
     1261 gcggcagctt tgcggcatcg tatcgttgcg gaacgtccgc atcgcgcctg aggcggcgaa
     1321 tgtcacggac gcaggtctgg cagacctgtg gcggctggta aatcttgaca gcctggccct
     1381 cgccgccccg caattcaccg gcagcgggct ggcgacgctc cacgagctgc cgaagctgca
     1441 gtggctgagc ctgtcgggcg ccggcatcgc ggacgttgcc tgcgagcacg ccgccaggtc
     1501 ggaatcgctt tcgcaactct ctctcggcgg ctggcaggaa ggcgggccgc aggggctcac
     1561 cgacgccggc ctgctgcacc tggctggcgc tcgcggcctg acgcagctcg atttgttccg
     1621 caagggaacc caggtgaccg acgccggcgt ggccgagctt cgcagccggc ggccgcagct
     1681 caagctgaac gcccatgggc cgtgaacact caacgcaaaa gcagtggata atgagcttcc
     1741 ttcgaaccac atggccgata ttgctcgccg cgctcggcgt atcggcaggc tgccggcgcg
     1801 cagaggtccc gcctggcaat cgccccgccg ccccgagcgt aacccccgtt gctctggaga
     1861 acccaccgcc agcggaagtc gccccacagc cggcccgtcc cgcgccgccg cccgccccgg
     1921 ctgtggattt caagacgttt gaagaccacg tcacgccgtt cctgaaaaag cactgccaca
     1981 agtgccacgg ggcggaacag gccgaagcca agctgcggct cgacacgctc gccgccgatt
     2041 tcctgcagcg gcccgccgcg gaccactggg tggaagtgct cgaccgcctg aatctcggcg
     2101 aaatgcctcc cgaggacgag ccgcggcccg ctgccgaaga tctggcggcg gtcaccgact
     2161 ggatcacctc ggagctgcgc ctggcccgcg agcggtccca gtcgaccggc ggccgcgtgc
     2221 tcctgaggcg gctgacgcgg ctggaatacg ccaacacggt gcgcgatctc ttgcaagtcg
     2281 aattcgctgg cggccagggg ccgctcgtgc agcttccgcc cgatggatcc cttgccggct
     2341 tcgaccgcgt gagcaaggcg ctgctgctcg atccgtcgct gatggaagcc tatctgtcgg
     2401 tcgcccagca agtggccgac caggcgatcg tcttccgccc gccgctggtg ccggagcgga
     2461 cgctgcgcta cgaattcgag cacaccgcct tcaatggcgc gatcagctac cagctttcgc
     2521 atcgcgccaa cgaattggac ggcccgttca tggtcgtgat ggaaaccagc gcgcggacct
     2581 acggtaagct gcggcatccc tatagcgaca aggagatccc gcttacgggc cggtaccgca
     2641 tccgcgtgcg cgccgcggcc gacccgggcg agcgcggaga gccggtcttc atggatgtga
     2701 cctacggcgc ggaggggctg caagggcgat tccgcgtcga ggccacaaag gacgcgccgc
     2761 aaatctacga attcgaaaag acgttcgacg ccttcacgcc gggcgagttc cacgtcggca
     2821 tcgccaatgg cacgcgcttc agttttggaa acgcggagtg gcacgaaagg aacaacgagc
     2881 tgacccggct ggccgaggaa ggcaacatgc tcgaggccac gcggatgaag gcccgcctgc
     2941 gggccgaagg aggctacgat cattatgtcc gcggctcgta cttgccggag gtgctgcaca
     3001 cggagacgct gcccaagctc tatctcgact ggatcgaagt catcggcccg ctgcagggcg
     3061 agtatccgcc tcctagcatg cacacggtgt tcggcgacgc cgagcgggcc gcccgattcg
     3121 tcgcaggcga taccagccgc gagcagttgc tggacgatgc ccgcgccgtc ttcgcgggcc
     3181 tgttgccgcg ggccttccgc cggccggtgc aggatgccga agtcgagcgg attgtgggcc
     3241 tggtcgccgc ggagctggaa gccggcgctg aaccgccgg
//
LOCUS       DASUGB010001038         6797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_671479, whole genome shotgun sequence.
ACCESSION   DASUGB010001038 DASUGB010000000
VERSION     DASUGB010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671479"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFV87_19635"
     CDS             <1..869
                     /locus_tag="VFV87_19635"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5106045.1"
                     /translation="SRLNQFTRVEIAGPEKLEWWRLVDRYLRPMPNVHLYVPVTREDI
                     LLRDIAVFDEHDASNALVQLMVPTKMPVVKHLHTRKLERAGYALFAHSQPFAHDVHEV
                     VIDLAQRGKIRQLRTAEDVTEYLGVAPDWNVIQKGEDVQIYFAYGSNLNPTQMSKRVM
                     KHRPVGVAYAKGFELAFSIPGSTWAGAVANIREEEGRFLYGVVYEIDAHGLDRLDFFE
                     GVKQGEYRREMITVYLHDRDAPVQAWVYRGEHRDAIAETPGKIYLDTIVEGAKSHGLP
                     PSYIKQLTGYQT"
     gene            complement(920..1888)
                     /locus_tag="VFV87_19640"
     CDS             complement(920..1888)
                     /locus_tag="VFV87_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5106046.1"
                     /translation="MHSAISQFLRFLSVERNAAELTIKSYREDLTSLADYLTQACGRT
                     PAPAEVTPLDLRGYVSALHEAGYAKTSVARRLASLRTFYKFAQREGLADSNPAKPLRN
                     PRPDRKLPHFLSTDEIGRLLQAPPATTPQGLRDRAILETMYSAGLRVSEAVGINDSDL
                     DLAEGLVRIRGKGRRERLAPIGSFAAKAIQRWQKVRKVGSDGLGGPGTANRRRVTAPV
                     PPRPSGPTPLFTNKFGKRLTTRSVHRLLLKYLKLTGLDLRTTPHTLRHSFATHLLDRG
                     ADIRSVQELLGHKSLVTTQIYTHVSTAGLRAVYEKAHPRARQKQRS"
     gene            complement(1981..2229)
                     /locus_tag="VFV87_19645"
     CDS             complement(1981..2229)
                     /locus_tag="VFV87_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106047.1"
                     /translation="MTQDINVLALVKGEERYIFLFDDEHRSEALRTLGKFASNPELSF
                     SWYDAAVLSQRIRQAAQEARAEEAETRPHPRFTLPVEE"
     gene            complement(2305..3363)
                     /locus_tag="VFV87_19650"
     CDS             complement(2305..3363)
                     /locus_tag="VFV87_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106048.1"
                     /translation="MHRHLKLLASIALTGMLCCAGSGCMLVPDRVVEPQFHNPYPQLH
                     RVAVLPFFNQSDEPTVDGEAIALAYYNELQLVPGFEVMPVGVAKQMLAASRIELRHGS
                     DFQKLAKLMNVDAVLVGSVTDYSPYYPPRIGLAVDWYAANPGFHPIPAGYGLPWGKAE
                     EEFIPQALVNEAEFALAREQLKTQTPDPAAAEVSSPHDGQEVAYRISDASGDVVLSPA
                     APADNTKAEPASSITSQLAAGMPAPALPPDWPDPRGFTPPAPSAERPPLRPQYDPIIT
                     HTRTYNGQDAKVTERLASYYYLRDDARFGGWSGYLQRPDDFARFCCYLHVTETLAARG
                     GAGEARVVWRWPIRRYER"
     gene            complement(3440..4129)
                     /gene="bshB1"
                     /locus_tag="VFV87_19655"
     CDS             complement(3440..4129)
                     /gene="bshB1"
                     /locus_tag="VFV87_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX5106049.1"
                     /translation="MLDILVIAPHPDDAELGMAGAILKFKAEGKKVGVLDLTSGEPTP
                     FGSLERRAAETAAATEILGLDWRENLGLPNRSLEATLEARGQLATVIRETRPRWLFAP
                     YWVDAHPDHVAATQLVEAARFWAKLSKTDMPGEPWHPERIYNYYCVHLKHAAQPAFVL
                     DISDYWDQKLAAIRCYHSQFIEGRSAEPPTFLDQLRDEAAYWGKTIGVRYGEPFHCRE
                     PIGLTTMQGLI"
     gene            complement(4135..4794)
                     /locus_tag="VFV87_19660"
     CDS             complement(4135..4794)
                     /locus_tag="VFV87_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106050.1"
                     /translation="MKVAIVSESSADEAAVGRLVAAVLKIEIETAGLKVRHRGWPNVC
                     QVLPNILREVYFATDAAGAIVVVDADSSPLHDNAHNAADPENAKCRICQLTQVVAGVV
                     PYLRQSPGRPHLNIAYGLAIPAIEAWLRCGVDAAVSEASWVNARRQNTFPYTTADLKR
                     AVYGTDRPSIDQETAAMTREAERLVAMNQIALLEKLFPLGFGLLASQLRAWNVAPPAA
                     G"
     gene            complement(4791..5804)
                     /locus_tag="VFV87_19665"
     CDS             complement(4791..5804)
                     /locus_tag="VFV87_19665"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5106051.1"
                     /translation="MLTYIRFQNFRVLKDTRLPLSRVTIIAGANGSGKSTVLQALSLL
                     SSNAQELGRFPSTKEYDGVVSVDAPSFQPPVEIQYYWDSAPPNNYIRLTWDKENRTHR
                     GSEPGHGIPAELQAFTTGIRVFALEPSAIAAPVQVGTNVQLTSSGGGLAGVLDNLQDN
                     TRERFDALNAELPSWIPEFDLVRFDQGPGVKSIKLRRKRDGLFIRAADLSEGTRFALT
                     LLTLAHMPDPPSLVGLEHPDAGIHPRLLRRVQDSIYRLAFPESHDDRRQPTQVVATTH
                     SPYFLELFKEHPEEIVFANKDENGVSFQRLSERPHIADILPEGPLGDLWYSGLLGGVP
                     SQP"
     gene            5928..>6797
                     /locus_tag="VFV87_19670"
     CDS             5928..>6797
                     /locus_tag="VFV87_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5106052.1"
                     /translation="MTTQSEHANSTNRPFFLGIDVGGTGIKLGLVDDQGKAVTHSRID
                     TEEENGPREAVRRAKQEVEAMLAGVGLGLTDVARVGLAVPGTMDIPRGMFLQPHNLPH
                     WHYFPIRDCVAEAFGLPVAFANDANAAAYGEFWVGSGQKYHSIVMLTLGTGVGGGIII
                     GDLSVDGEHSHGSECGHIIIDNSPAARMCGCGQPGHLEAYCSATALVKRVQELLDNGR
                     SSSLKALITPENPLTALMIAEAADSGDSLAFEAIMELGTWLGLGCVSLMHTIDPGAVI
                     LGGAMNFGGNEDPV"
BASE COUNT         1312 a         2109 c         2084 g         1292 t
ORIGIN      
        1 cgtcgcgatt gaaccagttc accagagttg aaatcgcggg gccagagaaa ctggaatggt
       61 ggagattggt tgaccgctac ctccggccaa tgccaaacgt ccatctctac gtccccgtta
      121 cacgggaaga cattctgttg agggacatcg ctgttttcga cgagcacgat gcaagtaatg
      181 cccttgtcca gttgatggtg ccaacgaaga tgcctgttgt gaagcatctt cacacgcgca
      241 agctagaacg cgcgggatac gcactattcg ctcactctca gccctttgcg catgatgttc
      301 acgaagtggt tattgacttg gcacagcggg gcaagattcg acaattgaga acggcggaag
      361 atgtgaccga atatctgggg gttgcaccag actggaatgt tatacagaag ggagaagatg
      421 tgcagatcta ctttgcatat ggatcgaacc tgaatccaac gcagatgagc aagcgtgtaa
      481 tgaagcaccg acccgttgga gtagcgtatg cgaaggggtt tgaattggca tttagcattc
      541 cagggtcaac atgggctgga gctgtggcga acattcgaga ggaagaaggc agattcctgt
      601 acggagtagt ttacgagata gacgcacacg gtcttgatcg gctggacttc ttcgagggcg
      661 taaagcaagg agaataccgc cgtgagatga ttaccgtcta tctgcatgac cgcgacgcgc
      721 cagtgcaagc gtgggtctac cgaggcgaac atcgtgacgc catcgctgag actcctggaa
      781 agatctatct cgacacaatt gttgagggag caaagagcca cggattaccc ccaagctaca
      841 tcaaacagct cacaggatat caaacctgag ctgtcggtgt agaaaacgag cgcgtggccg
      901 agcgatacca cgttcgcgat taagaccgct gcttttgcct tgctcgtgga tgggccttct
      961 catagacggc ccgcaagccg gcggtgctga cgtgcgtgta gatctgcgtg gtcaccaggc
     1021 ttttgtggcc gagcagttcc tggacgctgc ggatatcggc cccgcggtcg agcagatgcg
     1081 tggcaaagct gtgccgcagc gtgtgcggcg tcgtccgcag gtcgaggccc gtgagcttga
     1141 ggtacttgag cagcaggcgg tggacgctac gtgttgtcag acgcttgccg aacttgttgg
     1201 tgaagagcgg agtggggccc gacggcctcg gcgggaccgg cgccgtgaca cgacgtcgat
     1261 tcgcggttcc gggtccgccg aggccgtcgg aacctacttt gcgcaccttc tgccaccgct
     1321 gaatcgcctt tgccgcgaag ctgccgatcg gcgccagacg ctcgcgccgc cctttgcccc
     1381 ggatccgcac cagcccttcg gccagatcca gatcgctgtc gttgatgccg accgcttcgc
     1441 tcacgcgaag gccggcggaa tacatggtct ccaggatcgc ccggtcacgc agaccttgcg
     1501 gcgtagtcgc tggcggtgcc tgtaaaaggc ggccgatttc gtcggtcgaa agaaaatgcg
     1561 gcagcttgcg gtcgggccga ggattccgca gcggcttggc ggggttgctg tctgctagcc
     1621 cttcgcgctg cgcgaacttg tagaacgtcc gcagcgacgc caatcgccgg gccacagacg
     1681 tttttgcata ccccgcctcg tgcagcgccg agacatagcc gcgcaaatcg agcggcgtga
     1741 cttcagccgg tgcaggcgtc ctgccgcaag cttgcgtcag atagtcggcc agcgaggtca
     1801 ggtcctcgcg atatgacttg atcgtcagct cagccgcgtt gcgctcgacc gagaggaacc
     1861 gcaggaattg gctgatggca gagtgcatag taggttccga cggccacggc ggaaccaaaa
     1921 actgcgaatc gacgtcgccc cgtccccctt ggtcccgccc cggcggtcgg gacctacgtg
     1981 ctactcttcc accggcagcg tgaaccgcgg atgcggccgc gtttcggctt cttcggcccg
     2041 cgcttcctgg gccgcctggc ggattcgctg gctgaggacg gcagcgtcgt accagctaaa
     2101 gctcagctcg ggattcgacg cgaatttgcc cagcgtccgc aaggcttcgc tgcggtgctc
     2161 gtcgtcgaac agaaagatgt agcgctcctc gcctttgacc agcgccagca cgttgatgtc
     2221 ctgcgtcacg gggcggcttc tccttgcggt cagttgggcg tccctgcctg ggccgggctg
     2281 caagggcagc gcggactttt tcccttaccg ctcatatcgg cggatgggcc accgccacac
     2341 cacgcgcgcc tcgcccgccc cacctctcgc tgcgagcgtt tcagtcacgt gcaaatagca
     2401 gcaaaacctc gcaaaatcgt ccgggcgttg caaataaccg gaccagcctc caaaacgcgc
     2461 atcgtcgcgc aagtagtaat agcttgccag ccgctcggta acctttgcgt cttgtccgtt
     2521 gtaggtccgc gtgtgcgtga tgatcggatc gtattgcgga cgcagaggcg ggcgctccgc
     2581 actcggcgcg ggcggcgtaa agccccgtgg atcgggccaa tctggcggca atgccggcgc
     2641 tggcatgccg gcggcgagct gcgatgtgat gctgctggcg ggctccgctt tcgtattgtc
     2701 agcaggagcc gccggcgaaa gaacgacatc gccgctggca tccgaaatgc gataggcgac
     2761 ttcctggccg tcgtgcgggc tggaaacctc ggctgccgcc ggatcgggcg tctgcgtctt
     2821 gagttgctcg cgggccaggg cgaattccgc ctcgttcacc agagcctgcg gaatgaactc
     2881 ctcctctgcc ttgccccacg gcaggccata gccggccggg atcggatgaa agcctgggtt
     2941 ggccgcgtac caatcgaccg ccaggccgat ccgcggcgga tagtacggcg agtaatcggt
     3001 caccgatccc accagcaccg catcgacgtt catcagcttc gccagcttct gaaagtcgga
     3061 accgtgccgc agctcgatgc ggctggctgc cagcatctgc ttggcgaccc ccaccggcat
     3121 cacctcaaag cccggaacga gctgcagctc gttgtaatac gccagcgcga tcgcttcgcc
     3181 atcgaccgtc ggctcatcgc tctggttaaa gaacggcagc accgccacgc ggtggagctg
     3241 cggatacggg ttgtggaact gcggctccac gacgcgatcc ggcaccagca tgcacccgct
     3301 gccggcgcag cacagcatgc cggtcagggc gatcgacgct agcaacttga gatggcgatg
     3361 catgaggaga cggcgcgacc cgcgcgagga acctgcgcag cgtccccctt atcggcaaac
     3421 tccccgcagc ttctccaggc taaatcagcc cctgcatggt cgtcagccca atcggctcgc
     3481 ggcagtggaa gggctcgccg tagcggacgc cgatcgtttt gccccagtaa gccgcttcgt
     3541 cgcgaagctg gtcgaggaaa gtcggcggct cggccgatcg cccttcgatg aactggctgt
     3601 ggtagcagcg gatcgcggcc agcttctgat cccagtagtc gctgatgtcg agcacaaacg
     3661 ccggctgggc ggcgtgcttg agatgcacgc agtagtagtt gtagattcgc tcgggatgcc
     3721 acggctcgcc cggcatgtcg gtcttgctga gtttcgccca gaaccgggcc gcctcgacga
     3781 gctgcgtcgc cgccacgtga tcgggatggg catcgaccca gtaaggcgcg aacagccacc
     3841 gcggccgcgt ttcgcggatg accgttgcca actgcccccg cgcctcgagc gtcgcttcca
     3901 ggctgcggtt cggcagcccc agattctccc gccaatcaag gcccaggatc tccgtcgccg
     3961 cagccgtctc cgccgcccgc ctttccagcg aaccaaacgg cgtcggctcg ccgctcgtca
     4021 aatcgagcac gccgaccttc ttcccttccg ccttgaactt cagaatcgcc cccgccatgc
     4081 ccagttcggc atcgtcaggg tgcggagcaa tcacgaggat gtcgagcatg gcggctatcc
     4141 tgcggcgggt ggagcgacat tccacgctcg caattgactt gcgagcagtc cgaatcccag
     4201 cggaaacagc ttttcgagca gagcgatctg gttcatggcg accaagcgct ccgcctcgcg
     4261 cgtcatggca gccgtttcct ggtcgatcga gggtctgtcg gtcccgtaca ccgcgcgctt
     4321 caggtccgca gtcgtgtagg gaaacgtgtt ttggcggcga gcattgaccc aactcgcctc
     4381 ggagacagcc gcatccactc cgcagcgtaa ccaggcttcg atagctggga tcgccagacc
     4441 ataggcgatg ttcaagtgcg ggcgtccagg cgactgtctc aaataaggga caacgccagc
     4501 cacaacttgc gtgagctggc aaatgcgaca cttggcgttc tccggatcgg ccgcgttgtg
     4561 agcgttatcg tgcagcggcg acgagtcggc atcgaccacg acgatcgcac ccgccgcatc
     4621 agtcgcgaaa tagacctcgc gcaggatgtt ggggagcacc tggcaaacat tcggccagcc
     4681 acggtggcga actttgagac cagcggtttc gatctcaatc ttgagaaccg cagcgacaag
     4741 gcggcctact gccgcttcgt ccgccgacga ctcgctcacg atcgcgacct tcatggctgc
     4801 gacgggactc ctcccagaag accgctgtac cacaagtcgc ccagcggccc ctcgggcaga
     4861 atgtcggcga tatgggggcg ctcggacagt cgctggaagg atacaccgtt ctcgtccttg
     4921 ttggcaaaca cgatttcctc gggatgctcc ttgaacagtt ctaggaagta aggcgaatgg
     4981 gtcgtcgcga caacttgtgt cggttgccgc ctgtcgtcat gtgattccgg aaatgctagg
     5041 cgataaatcg aatcctgcac tctccgcaac aaccgtggat gaatcccagc gtccggatgc
     5101 tccagtccga caagggaagg cgggtctggc atgtgcgcca gcgtcaatag cgtcagagcg
     5161 aagcgagtgc cttccgagag atcggcagca cgaatgaaca atccatcgcg ttttctccga
     5221 agtttgatcg acttgacgcc gggtccctgg tcaaagcgta cgagatcaaa ctctggaatc
     5281 cacgacggaa gctcggcatt cagcgcatca aatcgctctc gcgtattgtc ctgaaggtta
     5341 tcgaggactc ccgccagtcc tccgccggac gatgtaagct gaacgttggt gccgacttga
     5401 accggcgctg caattgcgga gggttccaga gcgaatacac gaatcccagt cgtgaacgcc
     5461 tgtagttctg cgggaatgcc gtgtcctggc tcactgcctc gatgagttct attctccttg
     5521 tcccacgtca aacggatata gttgttggga ggagcgctat cccagtagta ctggatttcc
     5581 actggcggtt gaaacgaagg cgcgtccacg gatacaacgc cgtcgtattc cttggtcgaa
     5641 ggaaatcgcc ccaactcctg cgcattgcta ctcagtaggc taagcgcctg cagaaccgtg
     5701 ctcttccctg atccattggc ccccgcgatg attgtcacgc gcgagagcgg caatcgagta
     5761 tccttcagaa cacgaaagtt ttgaaagcgg atatatgtaa gcacggccaa ctccgaaata
     5821 ggcacgccat gggattcatc aaattgtaca cgtgttgact tcggattgca cttgctgcgg
     5881 tcttcacccg tcgacgtctt gcacgcctaa actgcccctt ttcccgaatg accactcaat
     5941 ctgagcacgc gaattcgaca aatcgcccct tcttcctcgg catcgacgtc ggcgggaccg
     6001 gcatcaagct ggggctggtc gatgaccagg ggaaggccgt cacgcactcc cggattgaca
     6061 ccgaggagga gaacggcccg cgcgaagcgg ttcgccgggc aaagcaggaa gtcgaggcga
     6121 tgcttgccgg cgttgggctc ggcttgaccg atgtcgcgag ggtcggactc gcggtgccgg
     6181 gcacgatgga cattccccgc gggatgttcc tgcagccgca caatctgccg cactggcact
     6241 attttccgat ccgcgattgc gtggccgagg cgttcggcct gccggtggcg ttcgccaacg
     6301 acgccaacgc cgccgcctac ggcgagttct gggtcggcag cgggcagaaa tatcacagca
     6361 tcgtgatgct cacgctgggc accggcgtcg gcggcggcat catcatcggc gacctctcgg
     6421 tcgatggcga gcacagccac ggcagcgaat gcggccacat catcatcgac aactcccccg
     6481 ccgcacgcat gtgcggctgc ggccagccgg ggcacctgga ggcttattgc agcgccacgg
     6541 ccctcgtcaa acgcgtgcag gaactgctcg acaacggccg ctccagcagc ctcaaggccc
     6601 tcatcacgcc cgagaatccc ctcaccgccc tgatgatcgc cgaagcagcc gacagcggcg
     6661 attcgctggc cttcgaggcg atcatggagc tcggcacgtg gctgggcctg ggctgcgtga
     6721 gcctgatgca cacgatcgat cccggcgcgg tcattctcgg cggggcgatg aacttcggcg
     6781 gcaatgagga ccccgtc
//
LOCUS       DASUGB010001039         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_950463, whole genome shotgun sequence.
ACCESSION   DASUGB010001039 DASUGB010000000
VERSION     DASUGB010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950463"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2803)
                     /locus_tag="VFV87_19675"
     CDS             complement(<1..>2803)
                     /locus_tag="VFV87_19675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEX5106053.1"
                     /translation="KYKDARIIFKDFEHSNWTWDPVAGAYYWHRFYSHQPDLNFDNPD
                     VHQAVFQALDHWLSMGVDGLRLDAIPYLFEREGTNCENLPETHTVLKKIRAHVDQHYP
                     DRMLLAEANQWPEDAVAYFGNGDECHAAFHFPVMPRMFMAVQMEDRFPIIDIMQQTPE
                     IPETCQWFMFLRNHDELTLEMVTDEERDYMYSVYAHNPQARINLGIRHRLAPLLGGHR
                     RKIELLNSLLMSLPGTPVIYYGDEIGMGDNIYLGDRNGVRTPMQWSPDRNAGFSDANP
                     QQLFLPVIIDHEYHYEAVNVETSRRNQFSLFWWMKRMIAMRQQAGVFSRGSLHFLLPE
                     NNRVLAFLRTLDHVTILVVANLSRFSQFVELDLSAFRGCTPIELFGQTPFPRIGELSY
                     LLTLGPHSFYWFSIQPVREPSLAVTEPAPAIAHAAVNLPALRASGHWQKVLEGRARES
                     LIRLLPEWLVKRRWFSGKAKAIRTATITDIVPLAGESVEFALLVVKVEYVNDFPETYV
                     LPVGFATGQPMDWLIGDNSPALMATLQVHTGNHDVSGVLYDAFGNEGLGQLVLEMIGH
                     RRRTQGRGGRLAGHPLRSYRTLRGAADEHLAVRALKVEQSNSTIIYGDRLVLKLFRRL
                     DDGVNPDLELSRFLTETAHFPHTPELAGYVDYHDQAGQPATVGVLQAFVRNEGDAWTY
                     TQESVHRFFDRVLTTRRNHPPQPAELPISGIVAASAEPLPQVARDLCNAYLESASLLG
                     QRTAEMHLALASSSDDPDFAVEPFSEHYQRSLYQGMRGLARKTLRTLRSKLGELPPET
                     QEEGRTLLLVEQELVERFRTIVGRKFSGARIRCHGDYHLGQVLFTGKDFVIIDFEGEP
                     ARRLSERRIKRSPLRDVAGMIRSFDYAAQTVLFNQMAGIVQKEEFPAFRQWALFWSQW
                     TSAQFLAAYAGKVSGASFAT"
BASE COUNT          504 a          829 c          967 g          503 t
ORIGIN      
        1 gcgttgcaaa gctcgcgccg ctgactttgc ccgcgtaggc ggcgaggaac tgcgcgctgg
       61 tccactgcga ccagaacagg gcccactggc ggaacgcggg gaattcttcc ttctgaacga
      121 tgccggccat ctggttgaag aggacggtct gggcggcgta gtcgaacgag cggatcatcc
      181 cggcgacgtc gcggagcggc gagcgcttga ttcgccgctc ggagagccgc cgggcgggtt
      241 cgccctcgaa atcgatgatc acgaagtctt tgccggtgaa gaggacttgc ccgaggtggt
      301 agtcgccgtg gcagcggatt cgcgcgccgc tgaatttgcg gccgacaatc gtccggaagc
      361 gctcgaccag ctcctgctcg accaggagca gcgtgcggcc ttcttcctgc gtttccggcg
      421 gcagctcgcc gagcttgctg cggagcgtgc ggagcgtctt gcgggccagg ccgcgcatgc
      481 cttgatagag cgagcgctgg tagtgctcgg agaacggttc gacggcgaaa tccggatcat
      541 cgctcgacga ggccagcgcc aggtgcattt cggccgtgcg ctggcccaga agcgacgccg
      601 attcgagata cgcattgcaa aggtcgcgag ccacctgcgg cagaggctcg gccgaggcgg
      661 ccacaatgcc gctgatgggc agctcggccg gttgcggcgg gtggttgcgg cgcgtcgtca
      721 gcacgcggtc gaagaagcgg tggacgcttt cctgcgtgta ggtccaggca tctccctcgt
      781 tgcggacgaa cgcctgcaaa acgcccacgg tcgcgggctg gcccgcctga tcgtgataat
      841 cgacgtagcc cgccagttcc ggcgtgtgcg gaaagtgggc ggtctcggtc aggaagcggc
      901 tcagctccaa atccgggttg acgccgtcgt ccagccgccg gaagagcttc agcaccaggc
      961 ggtcgccata gatgatcgtg ctgttgctct gctcaacctt cagagcgcgg acagccaggt
     1021 gctcgtcggc ggcgcctcgg agggtgcgat acgagcggag cggatgcccc gcgagcctgc
     1081 cgccgcggcc ttgcgtccgc cgccgatggc cgatcatctc cagcacgagc tgtcccagcc
     1141 cttcgttgcc gaaggcatcg tacagaacgc cggacacgtc atggtttccg gtgtgaacct
     1201 gcagcgtggc catcagggcg ggcgagttat ccccgatcag ccaatccatt ggctggccgg
     1261 tggcaaagcc gacggggagc acgtacgtct cgggaaaatc gttcacgtac tcgactttga
     1321 cgacgagcaa cgcgaattcg accgactcgc cggccagcgg cacgatatcg gtgatcgtgg
     1381 cggtgcgaat ggccttcgcc ttgcccgaga accagcggcg cttgacgagc cattccggca
     1441 agagccggat caatgattcg cgcgcccggc cttcgagaac tttctgccag tggccactgg
     1501 cgcggagcgc gggcaggttc accgcggcgt gggcgatggc gggggcgggc tcggtgacgg
     1561 ccagcgaggg ctcgcgcacc ggctgaatcg aaaaccagta gaagctgtgc gggccaagcg
     1621 tcaggaggta cgacagctcg ccgatccgcg ggaatggcgt ttggccgaac agctcgatcg
     1681 gagtgcagcc gcgaaatgcc gacaaatcca gctcgacgaa ctgggaaaac cgcgagaggt
     1741 tggcgacgac gaggattgtg acgtggtcga gcgtgcggag gaacgccagc acgcggttgt
     1801 tctcgggcag cagaaagtgc agcgatccgc gggaaaacac gcccgcctgc tgccgcatgg
     1861 cgatcatccg cttcatccac cagaagagcg agaactgatt gcggcgcgag gtttcgacgt
     1921 tgaccgcttc gtagtggtat tcgtggtcga tgatcaccgg cagaaagagc tgttgcgggt
     1981 tggcgtccga aaagccggcg ttgcggtcgg ggctccactg catgggggtg cggacgccgt
     2041 tgcggtcgcc gaggtagata ttgtccccca tgccgatttc gtcgccgtaa tagatgaccg
     2101 gcgtgccggg aagcgacatc aagagcgaat tgagcagctc gatcttgcgg cgatggccgc
     2161 cgagcagcgg cgccaggcgg tggcgaatgc ccaggttgat ccgcgcctgc ggattgtggg
     2221 cataaacgct gtacatgtag tcgcgctctt catcagtgac catctccagg gtcagctcgt
     2281 cgtggttccg caggaacatg aaccactggc aggtctccgg gatttccggc gtctgctgca
     2341 tgatgtcgat gatcgggaag cggtcctcca tctgcaccgc catgaacatc cgcggcatca
     2401 cggggaagtg gaacgcggca tggcactcat cgccgttgcc gaaataagcg accgcgtctt
     2461 ccggccactg gttggcctcg gccaggagca tgcgatcggg atagtgctga tcgacgtggg
     2521 cccggatttt tttcagcacg gtgtgcgttt cgggcaggtt ctcgcagttg gttccttcgc
     2581 gctcgaacag gtacggaatg gcgtccagtc gcaggccatc gacccccatc gagagccagt
     2641 gatcgagggc ctggaacact gcctggtgta cgtcgggatt gtcgaaattc agatcgggct
     2701 ggtggctgta aaagcggtgc cagtagtaag cgccggccac cggatcccag gtccagttcg
     2761 agtgctcgaa atccttgaag atgatccggg catccttgta ttt
//
LOCUS       DASUGB010001040         6530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_534207, whole genome shotgun sequence.
ACCESSION   DASUGB010001040 DASUGB010000000
VERSION     DASUGB010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534207"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFV87_19680"
     CDS             complement(<1..352)
                     /locus_tag="VFV87_19680"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5106054.1"
                     /translation="MLRRSALCRILPVFVPGLVWAFAATVQSAEPIVVTPAEVRLEGN
                     FARAQLVVSRPNADGRIDTRSEDLTNGASFTSSDPAVVTVSPAGQLRAIADGQAKISI
                     AVGDATQEVNVTVSG"
     gene            complement(549..974)
                     /locus_tag="VFV87_19685"
     CDS             complement(549..974)
                     /locus_tag="VFV87_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106055.1"
                     /translation="MKFSISQLLEITAIIGLTLALDRISTLWKASGFGHLIGIATVWG
                     ACTGAYLGFRWSKRRDIWAIMAAAGVAAAVATLINAARLGIMVAETLRTHYGYFNWTK
                     DWPTLLGFIFFITIVATALAPLLVIFPIICESVWRTLKK"
     gene            complement(1016..1906)
                     /locus_tag="VFV87_19690"
     CDS             complement(1016..1906)
                     /locus_tag="VFV87_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX5106056.1"
                     /translation="MPYEHVQSRPTTVALVQMSCTEAKQPNVEKAVARIGEAAGRGAN
                     VVCLQELFTGHYFCQSEDHRHFASAEPIPGPTSEALATAAKEHGVVVIGSLFERRAPG
                     LYHNTAVVLDADGSQAGVYRKMHIPDDPLYYEKFYFTPGDLGFQSFQTKFGKIGVCVC
                     WDQWYPEAARLTALTGAEILFYPTAIGWLPDEKEEFGASQHSAWETMMRSHAIANGVF
                     VAASNRVGREGKLQFWGGSFISDPNGNLLAKGQHEGEEIVLAQCDLGLIDVVRTHWPF
                     LRDRRIDAYGELTKRYRDLP"
     gene            2088..3227
                     /gene="queG"
                     /locus_tag="VFV87_19695"
     CDS             2088..3227
                     /gene="queG"
                     /locus_tag="VFV87_19695"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX5106057.1"
                     /translation="MRAEAHEHGFTLAGACPAVTPAGASRLGEWLGRGYGGDMDYLAE
                     RQAAYEHPRHVLDGVRSILMLGMPYRTEEPRPVQPGQGRVSRYAWGPGDYHDLIHERL
                     KRLIAVLQEAAPGAKARGVVDTAPLLEREFAQLAGLGWVGKHTLLINKPAGSYFFLAA
                     LLTDQELAYDEPFTADHCGTCRACLDACPTNAFPQPYVLDATRCISYLTIELREAIPE
                     DLRPGIGDWLFGCDVCQEICPWNQKPAPSPQPSPRGRGSDEPVLQPTTGTNPIDLIGL
                     FDLDDEGFRERFRHTPLWRAKRRGILRNAAIVLGNQRATAAIGVLTRGLDDAEPLVRG
                     ACAWALAQIGSGEAAIALSERLKVETDERVKRELALAALVLSTEY"
     gene            complement(3273..4103)
                     /locus_tag="VFV87_19700"
     CDS             complement(3273..4103)
                     /locus_tag="VFV87_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106058.1"
                     /translation="MFGAFSIFELLLVIAGGVLLLCLFPFVAGPVFIYFAHKQAARPE
                     LVPFTPGQTPLPANVDTYFSQTCWALDAEGFKIVTGMFLPKQIEHIVAGLVLVVNRQE
                     CDVAIVVAIHADPPGGMPFTQMHTEFVNRFRDGRLVQTANAQALSSFPPPANSVSSYL
                     PSVQDPRRLYHIHKALVKQHASSEKVLRLDEDFGGDAIRYAQFSLIEELNNACRVGFM
                     RLDLAAGVYRPTIKGACLMTWQEIFPLKNVRLVRRRRRERRLLEELAYQVPELAGIGK
                     "
     gene            complement(4349..4753)
                     /locus_tag="VFV87_19705"
     CDS             complement(4349..4753)
                     /locus_tag="VFV87_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106059.1"
                     /translation="MNLIHLLAEITATGRVAEASSSADGGFGWLWLIALVAIAGLSGV
                     ATWLIWRWRIARHRQSLNCPSQLLVELCKRHGLTSQERRLIAGLAREQDFEHPAMLFV
                     DPQAWETAQSGQLSGKHAAAIAALRQRLQASS"
     gene            complement(4781..>6530)
                     /locus_tag="VFV87_19710"
     CDS             complement(4781..>6530)
                     /locus_tag="VFV87_19710"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5106060.1"
                     /translation="CRNVGFSGVFRMSSGLPATFELLASTANEAAVPVLVVALDSSQG
                     DIRELAFSALLRRHSLAAEGEILRQWDSLCDRWKGQIAARDGWISGAIRQALLRSDPE
                     HYERGCAAAVFTRDYDLIPVLVAAAENKTSPHQTHAAATVLELAELLADELAAPRDYR
                     VRRDPRLQRQHLIGSLERAAAQFEQHGRRELLEALILLADRENAVLKRILQSPTERNF
                     VPLIETLVHSSRPAAMRLLLSYLDDPHAPLAALHALVRRRDISFLRQFARKVGSEPSR
                     TLRTNLHRIETIPWIADQLSLLDAFNESEQPGVVQIAALSGIARQSAFEVLAYVLRHG
                     KVAGRRVAARALAAFAGAAANQLALRSLQDEDPEVRASVARQLRDRGVPAAINRLIAL
                     LDSSHQCEREAAQQSLEEFRFSNFTANFDSLTEESRQATGPLVRRVDPQALVELRLEL
                     DAPSRGRRKRALEMVVAMDAVTVLHDAVAMLLKDEDQFLRVETVRVLATSDSTGTRAL
                     LRDALLDPHPLVAEAAEAALAQLAAVWASKTAQGQGPADIEMAEETASAQPQATEALA
                     ATTPWSELTAATNAES"
BASE COUNT         1220 a         2021 c         2119 g         1170 t
ORIGIN      
        1 tgccgcttac cgtgacattc acttcttgcg tcgcatcgcc gacggcgatg gagattttgg
       61 cctggccgtc agcgatcgcc cgaagctggc cagccgggct gaccgtgacg acggctgggt
      121 cagacgaggt gaagctcgcc ccgttcgtga ggtcttccga tcgcgtatcg attcgcccgt
      181 cggcattggg ccggctgacg acgagctggg cacgggcaaa gttgccttcc aggcgcactt
      241 cagccggcgt gacaacgatc ggctcggccg attggacagt ggcggcaaag gcccacacca
      301 atccggggac gaaaacgggc agaatgcggc aaagcgcgga tcgtcgcaac acagcggcgt
      361 cctccaagaa atcgaattca ggcgggagcg cggagcaggc aggatcgccc cgcgccattt
      421 caggcgggaa accagccagc cggagcagcc tccggccgac gcatcaatta tgcccgatca
      481 aacggcggat ggctacccaa acgcgcgaga aagtcggttt agatccaggg tcgatccatt
      541 cgcggcgttt acttcttgag agtccgccat acgctttcgc agatgatggg gaagattacg
      601 agtaagggcg ccagcgctgt agctacgatc gtgatgaaga agatgaagcc caagagcgtg
      661 ggccaatcct tcgtccaatt aaagtagccg tagtgcgttc gcagagtctc cgcaaccata
      721 attcccaaac gggcggcgtt tattagtgtc gccactgcgg cagcaacccc agcagcagcc
      781 atgattgccc aaatatcgcg tcgcttgctc catcgaaagc cgagatacgc ccccgtgcag
      841 gcaccccaaa cggtggcaat cccaataaga tgcccgaagc cgcttgcttt ccatagcgtt
      901 gagattcggt cgagagccaa ggtaaggccg atgattgcgg tgatctccag cagctgcgag
      961 atggaaaact tcatccgcat cgtccggcgc actccgggac caggtttggt cccggctaag
     1021 ggagatcgcg atagcgcttc gtcagctccc catacgcatc aatccgccta tcgcgcagga
     1081 acggccagtg ggtgcggacg acgtcgatga ggccgaggtc gcactgggcg aggacgattt
     1141 cttcgccttc gtgctgcccc ttggccagca ggttgccatt gggatcgctg atgaacgagc
     1201 ccccccagaa ttgcagcttc ccttcgcggc cgacccggtt ggaagcggcg acgaagacgc
     1261 cgttggcgat ggcgtggctc cgcatcatcg tttcccaggc gctgtgctgg ctggcgccga
     1321 actcctcttt ctcatccggc agccagccga tggccgtggg atagaagagg atctccgcgc
     1381 cggtcagggc ggtgagccgg gcggcttcgg ggtaccactg gtcccagcag acacagacgc
     1441 cgatcttgcc gaatttcgtc tggaagctct gaaagccgag gtcgcccggc gtgaaataga
     1501 atttctcgta atagagcggg tcgtccggga tgtgcatctt gcgatagacg cccgcctgcg
     1561 aaccgtcggc atcaaggacg acggctgtgt tgtgatagag gccgggggct cgccgctcga
     1621 agagcgagcc gatgacgacc acgccgtgct ccttcgccgc cgtggccagg gcctcgctgg
     1681 ttgggccggg aatcggctcg gcactggcga aatgccggtg gtcttccgac tggcagaagt
     1741 agtgccccgt aaacagctcc tgcaggcaga cgacattcgc cccgcggccg gcggcttcgc
     1801 cgatcctggc caccgccttc tcgacgttcg gctgtttggc ctcggtgcag gacatctgca
     1861 cgagggcaac ggtggtgggg cgggattgga cgtgctcgta gggcaaggga gtcggggatc
     1921 ggggttcggg gatcgggaat ttgtattcgt actattcgcg gcgtgcttgt gcgagtgggg
     1981 caagccgatc agattgcaat tgtatgcgag ccttcgccaa tcgacttatc atcgtgcccc
     2041 cgaaccccga atcccgaacc ccgagccccc aagcgctggt tgatctcttg cgggccgaag
     2101 cccatgagca cggcttcacg ctcgccggcg cttgcccggc ggtgacgcct gcgggggcga
     2161 gccggctggg ggagtggctg ggccgcggct acggcggcga catggactat ctggccgagc
     2221 ggcaggcggc ttatgagcat ccgcggcatg tgctggacgg cgtgcggagc atcctgatgc
     2281 tggggatgcc gtatcgcacg gaagaaccgc ggccggtgca gccggggcag gggcgcgttt
     2341 cgcgttacgc ctgggggccg ggcgactatc acgacctgat ccacgagcgc ctgaagcggc
     2401 tgattgcggt tcttcaggaa gcagcgccgg gggccaaggc ccgcggcgtc gtcgatacgg
     2461 ctccgctcct ggagcgggag ttcgcgcagc tcgcgggcct gggctgggtc ggcaagcaca
     2521 cgctcctcat caacaagccg gcggggagct acttcttcct cgcggccctg ctgaccgatc
     2581 aggagctggc ctacgacgag ccgttcaccg ccgaccactg cggcacgtgc cgggcctgcc
     2641 tcgacgcctg cccgaccaac gccttcccgc agccgtacgt gctcgacgcg acgcggtgca
     2701 tcagctacct gacgatcgag ctgcgcgaag cgattcctga ggacctgcgg ccgggaatcg
     2761 gcgattggct gtttgggtgc gacgtgtgtc aggagatttg tccatggaat cagaagccag
     2821 caccctcgcc ccagccctct cccagaggga gagggagcga cgaaccggtg ctgcaaccaa
     2881 caaccggcac gaacccaatc gatctcatcg gcctgtttga cttggatgac gaaggattcc
     2941 gcgagcgttt ccgccacacg cccctctggc gtgccaagcg ccgcggcatc ttgcgaaacg
     3001 ccgcaatcgt gctgggcaac cagcgcgcga ccgcggcgat tggtgtcctt acacgtgggc
     3061 tggacgatgc ggagccgctg gttcgtgggg cgtgcgcctg ggcactggcg caaattggca
     3121 gtggtgaagc ggccatcgcg ctcagcgagc ggctaaaggt ggaaacggat gaacgtgtga
     3181 aacgggagct cgcattggca gccctagtac tgagtactga gtactgagta cgtgtagtga
     3241 aggacggacc tggaagtccg tcctacggct gattacttcc caatccccgc caactccggc
     3301 acctggtagg ccaattcttc cagcagccgc ctctcgcggc ggcggcggcg gacgagacgg
     3361 acgtttttta gcggaaagat ctcctgccag gtcatcaggc acgcaccctt gatcgtcggc
     3421 cgataaacgc cggccgccaa atccagacgc atgaagccga cccggcaggc gttgttcaat
     3481 tcttcgatca gggaaaactg tgcatatcga atcgcatcgc cgccgaaatc ctcgtccagc
     3541 cgcaggacct tctcgctgct ggcgtgctgc ttcaccaggg ccttgtgaat gtggtagagc
     3601 cgccgcggat cctgcaccga aggcaaatac gagcttaccg agttcgcggg cggaggaaac
     3661 gaagacagcg cctgggcgtt ggccgtctga accagccggc cgtcgcggaa ccggttgacg
     3721 aactcggtgt gcatctgcgt gaagggcatt ccgccgggcg ggtcggcgtg gatggcgacg
     3781 acgatcgcta cgtcgcactc ctggcgatta accaccagca ccaggcccgc aacgatgtgc
     3841 tcgatctgct tgggcaggaa catgccggtg acgatcttga agccctcggc atcaagagcc
     3901 cagcacgtct gtgagaagta cgtatcgaca ttcgccggca ggggcgtttg tcccggcgtg
     3961 aacggcacca gttcgggccg ggccgcctgc ttgtgggcga aatagatgaa gacgggaccc
     4021 gcgacaaacg gaaacaagca gagcaatagc acaccgccgg cgatgacgag caacagttcg
     4081 aagatactga aggcgccgaa catggttgcg cgacgatgca actgcccgga cgtctcccgg
     4141 ctcagcgtgt cgctgactcc aatcgattat tgtaccgtcc ccgaccgccc aacaacttga
     4201 attcgtctcg cagccgttga tgctatcaat gggcgaaatg gggttgcgga atgtttggca
     4261 tggcaccggc gcaggtaggg tggaaggggt tattgccggc gagaggagcg ttgcttctct
     4321 ccctttcgct tcgcgcctcg tgatgtcctt aactcgacgc ctgcaagcgc tggcgaagag
     4381 cagcgattgc tgcagcgtgc tttccgctca gctggccaga ttgtgccgtt tcccacgcct
     4441 gtggatcgac gaataacatg gctggatgct cgaagtcttg ctcccgtgcc aggccggcaa
     4501 ttagccgccg ttcctggctg gtcagaccgt gacgcttgca cagttccacg agcaattggc
     4561 tggggcaatt caagctttgg cgatgacggg caatccgcca gcgccagatg agccatgtcg
     4621 cgacccctga cagtccggcg atggcgacca acgcgatcag ccacagccag ccgaagcccc
     4681 cgtcggcaga gctgctcgct tcggcaacac gaccggtcgc ggttatctca gccaatagat
     4741 ggatcagatt catttcgacc tcaaggactt gagccgggaa tcaagactcg gcattcgtgg
     4801 ctgctgtgag ttccgaccag ggcgttgtgg cggcgagcgc ttccgtggcc tgcggctggg
     4861 cgctggcggt ttcctcagcc atttcgatat cggctggccc ttgcccctgg gcggtcttcg
     4921 aggcccacac tgccgccagc tgagccagag ccgcctcggc agcctcggcc acgagcgggt
     4981 ggggatcgag cagggcgtcg cggagcaagg cccgcgtccc cgtgctgtcg ctggtagcca
     5041 ggacccgcac cgtttcgacc cgcaggaact gatcttcgtc cttcagcagc attgcgaccg
     5101 cgtcgtgcag tacagtcacg gcgtccatcg ccacgaccat ctccagggcc cgcttgcggc
     5161 ggcctcgcga gggggcgtcg agctccaatc gcaattccac cagcgcttgg ggatcgacgc
     5221 ggcgcaccag cggtccggtg gcctgccgcg actcttccgt gagactgtcg aaattggccg
     5281 taaagttgga gaagcgaaac tcttccagac tttgctgagc cgcttcccgc tcgcattggt
     5341 ggctgctgtc caataacgca atcaggcggt tgatcgcggc cggcacgccg cggtcgcgaa
     5401 gctgccgggc gaccgacgcc cgcacctcgg gatcttcgtc ctgcagcgag cggagagcca
     5461 attgattggc agctgctccg gcgaaggcgg ccagcgcccg ggctgccacg cggcggcccg
     5521 ccaccttccc gtgacgtaag acataggcca gcacctcgaa cgcagactgg cgggcaatgc
     5581 ccgacagcgc agcgatctga acgacgcccg gttgctcact ttcgttgaac gcgtcaagca
     5641 gcgaaagctg gtccgcgatc cacgggattg tctcgatgcg gtgcaggttt gtgcggagag
     5701 tccgggatgg ttcgcttcca accttgcgcg caaactgtcg caggaacgag atgtctcgcc
     5761 gccggacgag cgcgtgcagc gccgccagtg gagcgtgcgg gtcgtccaaa tacgaaagta
     5821 gcaatcgcat tgccgccggt cgcgacgaat ggaccaaggt ctcgatcagc gggacaaaat
     5881 tgcgctccgt cggcgattgc aggatgcgct ttaggacggc gttttcgcga tccgccagca
     5941 ggatcagtgc ttccagcagc tctcgacggc cgtgctgttc gaactgcgcc gcggcccgct
     6001 cgagacttcc aatcaggtgc tggcgctgca gccgcggatc gcggcgaacg cgatagtcgc
     6061 gtggcgcggc cagttcgtcg gccagcaact ccgccagttc cagcacggtt gctgccgcgt
     6121 gggtctggtg agggctcgtc ttgttttccg ccgcggcgac cagaacggga atgaggtcat
     6181 aatcgcgtgt aaaaacggct gccgcacaac cgcgctcata atgttcagga tcggatcgta
     6241 gtagtgcttg ccgaattgca ccagagatcc agccatcgcg ggcggcgatc tgccccttcc
     6301 agcggtcaca gagcgaatcc cactggcgga ggatctcgcc ctctgcggct aagctgtgcc
     6361 ggcggaggag cgcgctgaag gccagttcgc gaatgtcgcc ctgcgacgaa tccaacgcca
     6421 cgacaagcac cggcaccgcc gcttcattgg cggtcgaggc tagcaattca aaggtggcag
     6481 gcaggccgga tgacatgcgg aaaactcccg agaatcctac gtttctgcag
//
LOCUS       DASUGB010001041         6310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1366288, whole genome shotgun sequence.
ACCESSION   DASUGB010001041 DASUGB010000000
VERSION     DASUGB010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366288"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VFV87_19715"
     CDS             <1..971
                     /locus_tag="VFV87_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5106061.1"
                     /translation="AANQKTAEQQFAAFAALGLQYYSLRFIDAGQGIKNVMHLTKGEI
                     TKIRHLEDEYGLNVATLGSPIGKVKLLDQEDGTKNRHVPFKKYLNDEVKKACELAHAF
                     ETKLIRGFSFYHPKGTDPRPHLPQAIDQLGRIAELCHRSDLTFGLELEANLVGQTGEL
                     LAEIHKEVDHPALVTIFDGANMSTQGYSPGECFEQYEVMKPGLGWMHIKDYLHPQRMK
                     RTTHVDEEALKHFVPADVGDSGHELILRDFKTLIPTIEKKLKKRGVPGVFLDLEPHVK
                     GGGQFGGFSGPDGMGVALRGLCRVLDYVGIGYHLRDFDDVRVARGF"
     gene            1013..1243
                     /locus_tag="VFV87_19720"
     CDS             1013..1243
                     /locus_tag="VFV87_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106062.1"
                     /translation="MQSAVTIAAACPKCQGQSIECRFNYFDRGDLQIHSWEHRCPNCG
                     WRQTQAFRSDVPQAENLDPAACPWCSRKGEAS"
     gene            complement(1250..3940)
                     /locus_tag="VFV87_19725"
     CDS             complement(1250..3940)
                     /locus_tag="VFV87_19725"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5106063.1"
                     /translation="MPKTTTPPKRATPAHSLLGLGGELLTLRVLSGSHRGEVVQIRSS
                     KCTVGSAPGCTLRLRAAGVRPLHCWILRGPAGVIVRRRGAGTLLNGQKYDDTFLHNSD
                     RLRIGSVELEVLECGAKPTLPSTGWTAPVATSVAADPGPATESKIKDLESCLAAANDE
                     IARLEADAQQAWQTSIRASERAEQLHNALERAGVEAGELRAELEAAQAAQTQERDEAR
                     AKVLAVQQQLEQVRQEKIAAEQNTSREQQSWQQHSGELDRERQTLVRQLEEIKRQRDE
                     SERRRLEVEGQIQPLKAELAQWQSRVPLQTAQMTITMDQIRSEAQGEQDSAAAKWEER
                     VAELEGELTALKAELAEAVQQRGAAERREQTFREADQQVEHWQGEAHRRQLAADELQQ
                     QQAELQRQCELAQQQLTQVQQQLVQSQEQLAQALACQSQAEESARLLIEQEQDRLERL
                     SGEIAERQKQLESAECGCREQTEQLVHRHEALQAEIAARRSELEEKESLLIDQARQLQ
                     VRSEQLAAQEAETTQARDRIAASNAECEERERKLAEVESELLSGREALAVERMAFEQQ
                     IERFEAMSRSDQDQFPLPTSDDAEPSPLPMATMAFTPGSLAESLKAELLQGQILEGSD
                     SAASEPRESDEMNAVLDRLVRAGQWRTDDDEEPSAAETPPLPAPEPAPHAETSPPPAT
                     PHASQDEESIESYMERLMRRVRGDSPAAPSPAAPVVSSPSPVQELQEQPVQDIQDADI
                     RQTVRPEEYLPRTHPPEQSTNMAAMRELANSQARSAIESHRRRSVKQQFVGRLFAAGL
                     VFAASVLLIGCAIGAWSLLPAVAGVIGLGVSASWAFVALRRALASMRLEDPTGDLAKE
                     PEAGEAKAETGESADDSTAEPNDEPVADATSKDTPPGADS"
     gene            4097..4297
                     /locus_tag="VFV87_19730"
     CDS             4097..4297
                     /locus_tag="VFV87_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106064.1"
                     /translation="MRDTIAPSGRICRRAPNRLHDKAVFADYPLALTAGRKAASRVGS
                     SDVFGIAEVSERFLEVNSHQSA"
     gene            4557..5003
                     /locus_tag="VFV87_19735"
     CDS             4557..5003
                     /locus_tag="VFV87_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX5106065.1"
                     /translation="MARKDAMTALRQILIRRRDALRKALAGDLSLLKELRQQNSGDVI
                     DAALDSAQDEISSQLAEVESRELTHIETALERMRKGDYGVCDGCGEGIPLARLQALPY
                     ATMCIQCQREAEKTGELPGGANWNRIMDVGVADSDVSAGDLELDVG"
     gene            5075..6208
                     /locus_tag="VFV87_19740"
     CDS             5075..6208
                     /locus_tag="VFV87_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5106066.1"
                     /translation="MRARRLAADLFASGTLLLCIGFSIQGLRGDELTPTFDSGSSREQ
                     LFADLAADVAELERHGSILKRVVRLAKPSVVHIEARRDTEAGRRSGDVEEAGSGVIVE
                     RGGQHYVLTNRHVIKYSSVAAIDVKLADGRILHPTKVWADKATDIALLAIASGDLVPA
                     RLGDASRLDIGDFVLAVGSPFGLSHSVTYGIISAKGRRDLALGDDGVQLQDFLQTDAA
                     MNPGNSGGPLLNLRGEVVGINTAIASSSGGNEGIGFAIPINMVMVIAGQLIDRGEVVR
                     AYLGVRLDSAFGPEAAARLGLSRPGGAHVTGITKDSPAELANIAVNDVILEFDGTRIE
                     DDNHLINLVGLTPVNKEVEVRLLRSGRPMTIRVRVGNREFSER"
BASE COUNT         1090 a         2038 c         1967 g         1215 t
ORIGIN      
        1 aagcggcgaa ccagaaaacc gctgagcagc aattcgccgc cttcgcggct ttgggtttgc
       61 agtactattc gctgcggttc atcgacgccg gtcagggcat caagaacgtg atgcatctga
      121 cgaagggcga gattacgaag attcgccacc tggaggacga atatggcttg aatgtcgcca
      181 cgctcggctc gccgatcgga aaagtgaagc tgctcgacca ggaagacggc acgaagaacc
      241 gccatgtgcc gtttaagaag tatttgaacg acgaagtaaa gaaggcctgc gagctggcac
      301 acgcattcga gacgaagctg atccgcggat tctcgttcta tcaccctaag ggaaccgatc
      361 cgcgtccgca tctgccgcag gcgatcgatc agctcggcag gatcgccgaa ctgtgccacc
      421 gcagcgacct gacattcggt ctggaactgg aggcgaatct cgtcgggcaa acgggcgaat
      481 tgctcgcgga aatccacaag gaagtcgatc acccggccct ggtgacgatc ttcgacgggg
      541 cgaacatgag cacgcagggc tacagccccg gcgagtgctt cgagcaatac gaggtgatga
      601 agcccggcct cggctggatg cacatcaagg actatctcca cccacagcgg atgaagcgga
      661 ccacgcacgt cgatgaagag gccctcaagc acttcgtgcc cgccgacgtc ggcgacagcg
      721 ggcacgagct gatcctccgc gacttcaaaa cgctgattcc gacgatcgag aagaagctga
      781 agaaacgcgg cgtgcctggc gtattcctcg acctggagcc gcacgtcaaa ggcggcggtc
      841 aattcggggg cttcagcggg ccggatggca tgggcgtggc cctgcgggga ctgtgccgag
      901 tgctcgacta cgtcggcatc ggctatcact tgcgcgattt tgacgacgtg cgggtggcgc
      961 gggggttctg agcggggcag gcttccaacc tgtcatgctc gatgagcagc acatgcagtc
     1021 cgccgtaacc atcgccgcag cttgcccgaa gtgccagggc caatctatcg agtgccgctt
     1081 caactacttc gaccgcggcg atttgcagat ccactcgtgg gagcatcgct gcccgaactg
     1141 cggctggcgg cagacgcagg cgttccgatc ggatgtgcca caggcggaaa atctcgatcc
     1201 cgcggcgtgt ccctggtgca gccggaaggg cgaagcgagt tgagcttgat tacgagtcag
     1261 ctcctggcgg cgtatcctta gaggtcgcgt cggccaccgg ctcgtcgttt ggttcggccg
     1321 tagagtcgtc agccgactct cctgtttccg ctttcgcctc gccggcttcc ggctccttcg
     1381 cgagatcccc cgtcggatcc tcaagccgca tcgaagccag tgcccgccgc aaggcgacga
     1441 acgcccagga agcgctgaca cccaggccaa tgacgccggc cacggcgggc aacagcgacc
     1501 aggcgccaat cgcgcaccca atcagcaaca cacttgcggc aaacaccagt ccggcggcaa
     1561 acaagcggcc gacaaactgc tgcttgaccg agcggcggcg atggctctcg atcgcggagc
     1621 gggcctggct gttcgccagt tcacgcatcg ccgccatgtt cgtcgattgt tccggcggat
     1681 gggtccgtgg caagtactct tcgggcctca cggtctgacg gatatccgcg tcctgaatgt
     1741 cttgaactgg ctgctcttgc agctcctgca ccggtgaagg cgaactgacg acaggagctg
     1801 caggcgaagg agccgccggg ctatcgccgc ggacgcgccg catcaggcgt tccatgtacg
     1861 actcgatgga ctcttcatcc tgcgaagcat gcggtgtggc gggcggcgga ctcgtttccg
     1921 cgtgcggcgc gggctctggc gccggcagcg gcggcgtctc ggccgcgctc ggctcttcat
     1981 cgtcgtccgt ccgccactga ccggcgcgca ccagccgatc gagtacggca ttcatttcgt
     2041 cggactcccg cggctcgctg gcagcagaat ctgatccttc caggatctga ccctgcaaga
     2101 gctcggcttt cagggattct gcgaggctgc ctggtgtaaa agccatcgta gccatcggga
     2161 gcgggcttgg ttcggcgtcg tccgatgtgg gcaagggaaa ctgatcctgg tcgctgcggc
     2221 tcatcgcctc gaaccgctcg atctgctgtt caaacgccat ccgctcgaca gcgagcgctt
     2281 cgcggcccga gagcagctcg gattcgacct ccgccagttt ccgctcgcgc tcctcgcatt
     2341 cagcgtttga cgccgcgatt cgatcgcggg cctgcgtcgt ttccgcttcc tgtgcggcaa
     2401 gctgctccga ccgtacctgg agttgccggg cctgatcgat cagcagcgat tccttctcct
     2461 ccaactcgct gcgcctcgcg gcgatttccg cctgtagcgc ttcgtgccga tggacaagct
     2521 gttcggtttg ctcgcggcag ccgcattcgg cggattcgag ctgcttctga cgttctgcta
     2581 tttcgccgct cagccgctcc agacgatcct gttcttgctc gattagcagg cgggccgatt
     2641 cttcggcttg cgactggcac gcaagggcct gcgccaattg ctcctgtgac tggaccaatt
     2701 gctgctgcac ttgagttagt tgctgctgcg ccaactcgca ctgccgctgg agctccgcct
     2761 gctgttgctg caattcatcg gccgcgagct gacggcgatg agcttcgcct tgccagtgct
     2821 cgacctgctg atcggcttcg cgaaacgtct gttcccggcg ctcagccgcg ccgcgctgct
     2881 gcactgcttc ggccagctcc gccttcagcg cggtcagctc gccttccagt tccgcaacac
     2941 gctcttccca cttcgcggcg gcgctgtcct gctcgccctg cgcttcggac cggatctggt
     3001 ccatcgtgat tgtcatctgc gccgtctgga gcgggacgcg gctttgccat tgagcgagct
     3061 cagccttgag cggctggatt tgcccctcga cttccaggcg ccggcgctcg gattcgtctc
     3121 gctggcgctt gatttcttcg agctggcgca ccagcgtctg ccgctcgcgg tccaactcgc
     3181 cagagtgttg ctgccaactt tgctgttctc gtgaagtgtt ctgttccgcg gcgatctttt
     3241 cctggcggac ctgctcgagc tgctgctgga cagccagcac tttcgcacgc gcttcgtcgc
     3301 gctcttgcgt ctgggccgct tgtgccgctt ccaactcggc ccgcaactca ccagcttcga
     3361 cgccggcacg ttccaaggcg ttatgtagct gctcggcacg ctcggacgcg cggattgaag
     3421 tttgccaggc ctgttgcgca tcggcttcca gccgcgcgat ctcatcgtta gccgccgcca
     3481 ggcagctttc gaggtccttg atcttgctct ctgtcgccgg tcctggatcg gcggctacgc
     3541 tggtcgccac cggcgcggtc cacccggtac ttggcagcgt gggcttggcg ccacactcca
     3601 gcacctccag ctccaccgat ccgatccgca accgatcgct gttgtgcaag aacgtgtcgt
     3661 cgtatttctg gccgttcagg agcgtccccg cgccgcggcg gcggacgatg acgccggccg
     3721 ggccgcggag aatccagcaa tgcagcggac gcacgcccgc cgctcgcagc cgcaacgtgc
     3781 accccggcgc ggagcccacc gtgcacttcg acgaacggat ctgcactacc tcccctcgat
     3841 gcgagccgct caagacgcgg agcgtcagca gctcgccacc gagtcccagc agggaatgcg
     3901 caggtgtcgc gcgttttggg ggagttgttg tcttaggcat tgcggacagc ccttttgcca
     3961 ggccttcgtg cgagcagggg agcgacgcca aggcgcggaa cccctcctgg gcatgtagct
     4021 tgcgccagcc ctgccgtcaa agttgacgtc tccccggtcc tttcgcggaa aagtgccggc
     4081 aggtgaggga gtccgcatgc gcgacaccat cgcgccaagc ggcagaattt gccgccgcgc
     4141 tccaaatcgg ctgcacgaca aggccgtttt tgccgattat ccgttagcgt taaccgctgg
     4201 ccgcaaagcc gcatcccggg tgggctcttc agacgtcttt ggcattgccg aagtgtctga
     4261 gcgcttcttg gaagtgaatt cgcaccagtc ggcgtagcat gcgaaggcag cacgcaggtc
     4321 gttgtaacgt cctgtgcagc aatgcgttac gatgacttgc ctgcgtcgca gcaaggcgaa
     4381 agctctggcc ccattcgaag gccggcagta gccgccatgc gtactatatt tcgggtgctc
     4441 tggcaccctt gtcggcagcg ggttgggcct gctcgtcgaa aggggtcagc gcgacgggca
     4501 acagagattc caggcaaccg gctgccgggc gcaccggcgg ctgcaggaac cgtacgatgg
     4561 cgcgaaaaga cgcaatgacg gctttgcggc agatcctcat tcgccggcgc gatgcactgc
     4621 gcaaggccct ggcaggcgat ctgagtctgc tcaaggagct gcggcaacag aactcgggcg
     4681 acgtgatcga tgccgctctc gattcggccc aggacgaaat cagctcccag cttgccgaag
     4741 tcgagagccg agagctgacg cacatcgaaa ccgccctgga gcggatgcgc aagggcgatt
     4801 acggcgtttg cgacggctgt ggcgagggca ttccactggc tcgcctgcag gccctgcctt
     4861 acgccaccat gtgcattcag tgccagcgag aagcggaaaa aaccggcgaa ttgccgggcg
     4921 gcgcgaattg gaaccgaatc atggacgtgg gcgtcgccga cagtgacgtc tctgctggcg
     4981 atctggagct cgacgtcggc taggctggat caagtcgcag gacgggtgtc caacccatcc
     5041 gcccgtcagc gtttgcaagt agcaaggaag tgcgatgcgc gctcggcgat tggctgctga
     5101 tcttttcgca tccggcacgc ttctgctgtg catcggattc tcaattcaag gcctgcgcgg
     5161 cgacgagctg acgccgacgt tcgactccgg ctcgtcccgc gagcagttgt ttgccgatct
     5221 ggctgccgat gttgctgagc tggagcgtca cggcagcatt ctcaagcgcg tcgttcgcct
     5281 ggccaagccg tccgtcgttc acatcgaggc ccgccgggac accgaagcgg gtcgtcggag
     5341 cggcgatgtt gaggaagccg gctcaggcgt catcgtggag cgcggcggtc agcactacgt
     5401 gctcaccaac cgacatgtca tcaagtactc gtccgtcgcg gcaatcgatg tcaaactggc
     5461 cgatggccgt atcctgcatc ccaccaaggt ctgggccgat aaagcgacag atattgcgct
     5521 cttggcaatc gcgtctggcg acctcgtgcc cgctcgcctg ggagatgcca gccggctgga
     5581 catcggcgac ttcgttctgg ccgtcggcag cccgttcgga ctgagccact cggtcaccta
     5641 cggcatcatc agcgccaagg gacgtcgcga tctggccttg ggcgacgacg gcgtgcagct
     5701 tcaggatttt ttgcagaccg acgcggccat gaatcccggc aatagcggcg ggccgctcct
     5761 caatcttcgc ggcgaagtcg tcggcatcaa caccgcgatt gccagctcca gcggcggcaa
     5821 cgagggcatc ggcttcgcca tcccgatcaa catggtgatg gtgattgccg gccagctcat
     5881 cgaccgagga gaggttgttc gcgcgtattt gggcgtgcgg ctcgattctg ccttcggtcc
     5941 cgaggccgcc gctcggctgg gcctttcgcg tccggggggg gcccacgtca cgggcatcac
     6001 gaaggattct ccggccgagc tggcgaacat cgcggttaac gacgtgatcc tggaattcga
     6061 cggaacgcga atcgaagacg acaatcacct gatcaacctt gtcggcctga cacctgtcaa
     6121 caaagaggtc gaagtgcgcc tcctccgcag cggccggccc atgaccattc gcgtccgcgt
     6181 cggcaaccgc gaattttccg aacgatagcc gccgcgccgt gtagccatct cgctccacaa
     6241 gaagagcgcc cctaagcttc caagggctac atacgacgac tgtgtctgcc acgagcttca
     6301 cgagcgcatc
//
LOCUS       DASUGB010001042         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_534571, whole genome shotgun sequence.
ACCESSION   DASUGB010001042 DASUGB010000000
VERSION     DASUGB010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534571"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFV87_19745"
     CDS             complement(<1..725)
                     /locus_tag="VFV87_19745"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX5106067.1"
                     /translation="MRSARLMLDPPAPGAWNMAVDEVLLDSAATAGIATLRFYQWSEP
                     TLSLGYFQAAAGREQHPASRACPLVRRASGGGAIVHHRELTYSIALPLRDSRSQAATD
                     LYNACHESLVAALAELGVTAEMYQPAESCAVSAESHANRPFLCFERRACGDIVCGTSK
                     IVGSAQRRRRGAVLQHGSILLGRSACAPELPGIEQFTRQSITAGQIIHDWPNRLAARL
                     QLAIAPARLTSDEHQNAATIADSR"
     gene            complement(718..2259)
                     /gene="gcvPB"
                     /locus_tag="VFV87_19750"
     CDS             complement(718..2259)
                     /gene="gcvPB"
                     /locus_tag="VFV87_19750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5106068.1"
                     /translation="MRNLKSTTLLFELSQPGRRAAMLPACDCSAPPASELLPAEALAA
                     GPPPLPEVAEPEVIRHFTNLSTLNMSVDTHFYPLGSCTMKYNSKRNERMAALPGIADL
                     HPLAPEETIQGLLQLLFELQQMLAEISGLPCVSLQPAAGAHGELAALMVAAAYFRHNK
                     LGRTKVLAPDSAHGTNPASARMAGFDFVTVKSRGDGMVDLEDLQAKLDDQIAVFMITN
                     PSTLGLFDRQVPRIAELVHERGGLIYLDGANMNAILGITRPGDFGADMMHYNPHKTFS
                     GPHGGGGPGAGPICVAGKLGAFLPTPVVVRTEGTGDRGQGTGETAENPKSKIQNPKSA
                     ATFRLEYDRPHSIGRVRSFFGNTGVLVRAYCYLRTHGPEGLKAVAENAVLNANYLLSR
                     VKHILPVPHGERCMHEFVATAAPLKKKNGVTAMDLAKRLLDYGFHAPTVYFPLTVPEA
                     MMIEPTETESKETLDAFAEVLFRITEESPELLHEAPHSTIISRPDEVRAARQPVLKWT
                     AESHA"
     gene            complement(2271..2633)
                     /locus_tag="VFV87_19755"
     CDS             complement(2271..2633)
                     /locus_tag="VFV87_19755"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX5106069.1"
                     /translation="MRIYLDEDIAAGQLLTEIRRAGHDVQVPGDAGLLGESDPVQFAH
                     AIRDGGDVMTGNHHDFDELHSLILVAGGKHPGALVIRKDDDRRRDMTNRQIVQALTRL
                     QATAPVHENQLFILNHWR"
     gene            complement(2617..3015)
                     /locus_tag="VFV87_19760"
     CDS             complement(2617..3015)
                     /locus_tag="VFV87_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106070.1"
                     /translation="MSIIGNNSSSRPADALAQQYKYLAPKPKSAYKQLFVKGRGIFAR
                     TLYGAYMSEEMPMTPEEIAADRDLPVEAVLEAIAYCESNPPEIEEDFRREEALMKATG
                     LDRQSPSTPYTYQPLTPQQRAAIHRNANLP"
     gene            complement(3023..>3427)
                     /locus_tag="VFV87_19765"
     CDS             complement(3023..>3427)
                     /locus_tag="VFV87_19765"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913572.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEX5106071.1"
                     /translation="TLFALRAAIYLAQLGPRGLADTAELCLRKAHYAKEQLGKSPRLS
                     LAFGQPTFKEFVVRDRDGRVPDLIAQARDAGYLAGIPLGQWYPQLDDCFLVTVTEKRT
                     KAEIDGLAEVLSARRDSSGSSSTPARPAVANV"
BASE COUNT          589 a         1063 c         1126 g          649 t
ORIGIN      
        1 cgggaatctg cgatggttgc ggcgttttgg tgctcgtcgg aagtaagtct ggcaggggcg
       61 attgcgagct gaagccgagc ggcgaggcgg ttcggccagt cgtggattat ttgtccggcc
      121 gtgatggatt gccgggtgaa ctgctcgatg cctggcaact ccggagcgca ggccgagcgg
      181 ccgagcaaga tgctgccgtg ctgaagcact gccccgcgcc ggcggcgctg ggcgctgccg
      241 acgatcttgc ttgtgccgca gacgatatcg ccgcacgctc gccgctcaaa gcagaggaac
      301 ggccgattcg cgtgcgattc ggcgctcacg gcgcaagatt cggcgggctg gtacatctcc
      361 gccgtcacgc cgagttcggc gagcgccgcg acgagcgatt catggcaagc gttgtacaga
      421 tcggtcgccg cctgcgagcg gctgtcgcgc agcgggagcg cgatgctata ggtcagttcg
      481 cgatggtgga cgatcgcgcc gccgccgctc gctcgccgca cgagcgggca agcgcggctg
      541 gctggatgct gctcgcggcc ggccgcggcc tgaaagtagc cgagcgagag cgtcggctca
      601 ctccactggt agaaccgcag cgttgcgatt cccgcagtcg ccgccgagtc gagcagcacc
      661 tcatcgaccg ccatgttcca ggcgccaggc gccggcggat cgagcattag ccgggcacta
      721 cgcatgcgac tccgccgtcc acttcagcac cggctgccgc gcggcccgga cttcgtccgg
      781 ccggctgata atcgtgctgt gcggcgcttc gtgcagcaat tcgggcgatt cctcggtaat
      841 gcggaacagc acctcggcga aggcgtcgag cgtttccttg ctctccgttt ccgtcggctc
      901 gatcatcatc gcctcgggca cggtgagcgg gaaataaacg gttggcgcat gaaagccata
      961 gtccaagagc cgcttggcca aatccatcgc cgtcacgccg ttcttctttt tcagcggcgc
     1021 ggccgtggcg acgaactcgt gcatgcaccg ctcgccgtgc ggaaccggca ggatgtgctt
     1081 cacgcggctg agcaggtaat tggcgttgag gacggcgttt tccgcgaccg ccttgaggcc
     1141 ctccggccca tgggtgcgca gataacaata ggcccgcacg agcactccgg tgttgccgaa
     1201 gaagctgcgg acgcgaccga tggaatgcgg gcggtcatac tcgagacgaa atgtggcggc
     1261 ggattttgga ttttggattt tggattttgg attctcggct gtctcccctg tcccctgtcc
     1321 cctgtcccct gtcccctcgg ttctgacgac caccggagtc ggcaagaacg ccccgagctt
     1381 ccccgcgacg caaatcggcc ccgcgccggg gccgccgccg ccgtgtgggc cgctgaaggt
     1441 cttgtgcggg ttgtagtgca tcatgtccgc gccgaagtca cccggccgcg tgatgccgag
     1501 gatcgcgttc atgttcgcgc catcgagata gatcaagccg cctcgctcgt gtaccagctc
     1561 cgcgatccgc ggcacctgcc gatcgaacag ccccagcgtg ctggggttgg tgatcatgaa
     1621 gacggcaatc tggtcgtcga gttttgcttg caagtcctcc agatcgacca tgccgtcgcc
     1681 gcggctcttg accgtgacga agtcgaagcc ggccatccgc gcgctcgcgg gattcgtgcc
     1741 atgagcactg tccggcgcga gcactttggt tcggcccagc ttgttgtgac ggaagtaggc
     1801 cgccgcgacc atgagtgcgg cgagctctcc atgcgctccg gccgccggct gcagcgaaac
     1861 gcagggcagc cccgaaattt cggccagcat ttgctgcagc tcgaacaaga gctgcaaaag
     1921 tccctggatc gtctcttcgg gcgccagcgg gtgcagatcg gcgatgcccg gcaaagcggc
     1981 catccgctcg ttccgcttgc tgttgtactt catcgtgcaa ctgccgagcg gatagaagtg
     2041 cgtatcgacc gacatgttaa gggtcgagag attcgtgaag tgccggatca cctccggctc
     2101 ggccacctcc ggcaagggtg gcggaccagc cgcgagcgcc tcagccggca gcagctcgct
     2161 ggccggcggc gcgctacaat cgcacgccgg cagcatcgcc gccctgcggc ccggctgaga
     2221 aagctcaaac aggagcgtgg tggatttgag gttgcgcatt tcgatgtcgc ttagcgccaa
     2281 tgattgagaa tgaacaactg gttctcatgc accggagcag ttgcctgaag ccgagttagg
     2341 gcttgaacaa tctgtctgtt ggtcatgtcg cgacgtctgt catcgtcctt gcggatgacg
     2401 agagcgcccg ggtgcttgcc gccagcaacc agaatcaggc tatgcagctc gtcgaagtcg
     2461 tggtgatttc cggtcatcac gtcgccgcca tcacgaattg catgcgcgaa ctgcacgggg
     2521 tcgctttcgc cgagcaagcc cgcgtcgcca ggaacctgaa cgtcatggcc tgcgcggcgg
     2581 atttcggtaa gcagttgccc ggccgcgatg tcttcatcaa ggtagattcg cattccgatg
     2641 aatcgcggcg cgttgttgcg gagtcaatgg ctggtaagta tagggcgtag aaggcgattg
     2701 gcgatcaagc ccggtggcct tcatcaatgc ctcttcacgg cggaaatcct cctcaatttc
     2761 cggaggattc gactcgcaat acgcaatcgc ctccagcacc gcctccacgg gcaagtcgcg
     2821 gtcggcggca atctcctcgg gcgtcatggg catctcctcg ctcatgtagg ccccatacaa
     2881 ggtccgcgca aagatccccc gacctttgac aaacaactgc ttgtacgccg atttgggttt
     2941 cggagcgagg tatttgtatt gctgtgcaag cgcgtcagcc ggacgggacg acgagttgtt
     3001 gccgatgatg ctcatatgga gattatacgt tggccacggc agggcgcgct ggagtgctgc
     3061 tggagccaga cgaatcacgc cgggcggaca gcacttccgc caggccgtcg atttctgcct
     3121 tcgtccgctt ctcggtcacg gtcaccagga agcagtcgtc gagctgcgga taccactggc
     3181 cgagcggaat accggccaga tagccggcgt cgcgtgcctg agcgatcaaa tcggggacgc
     3241 ggccgtcgcg gtcgcgaacg acgaactcct tgaacgttgg ctggccgaag gcgagcgaga
     3301 ggcgcggcga cttgccgagc tgctccttcg cgtagtgcgc tttgcggagg cacagctcgg
     3361 cagtatccgc caggccgcgc gggccgagct gcgccaggta aatcgcggcg cggagggcga
     3421 agagcgt
//
LOCUS       DASUGB010001043         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1646503, whole genome shotgun sequence.
ACCESSION   DASUGB010001043 DASUGB010000000
VERSION     DASUGB010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646503"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VFV87_19770"
     CDS             complement(<1..1336)
                     /locus_tag="VFV87_19770"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106072.1"
                     /translation="MTTNRLPLVSRRKMLAQSAAGFGTIGLAGAMHAAGLIGAPAQAA
                     NEPAYPSPHFAPRAKRVIYLFMNGGPSHVDTFDPKPALKDHEGQMPEGDTMRKKKGGF
                     IPSPFQFAAHGESGVVMSELFPNLARHADDLCVIRSMHTDVPNHEPGLLLMHSGNQQP
                     IRPSLGAWVSYGLGTENQNLPAFVVLAPRRPVVGPHLWSASFLPAAHQGMSVDTNDMQ
                     VEKLVTNLRHPTLSRDEQREQVDLLAALNRLHLAERPGDSGLEAQIQAMETAFSMQAE
                     AAEAFDIGREPESVRAAYGQTNFGQQCLLARRLCEHGVRYVSVYYLSKDNNQPWDTHA
                     DNDNRHRKLCEDADRATTALLTDLRQRGLLEDTLVVWGGEFGRTPYAEKQDKGKPGRD
                     HHHTGFSILMAGGGVKGGLMYGATDEVGMHAVENRVHIHDLHATILHLLGLDH"
     gene            complement(1345..>2108)
                     /locus_tag="VFV87_19775"
     CDS             complement(1345..>2108)
                     /locus_tag="VFV87_19775"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5106073.1"
                     /translation="IVNRLWGLYMGRPLVATPSNYGLRGDFPTHPELLDDLAVRFMDR
                     GWSLKWLAREVVLSSAYRQSSNSQSAIRDPQLADPENRLLAHMNRRRMTVEQWRDSLL
                     MAAGRLDTAIGGPSLDPQNPEERRRTIYSQASRLELNRMLALFDYPDPNVHADRRSET
                     TTPLQKMFVLNSQFMIEQAASLALRQQDESLDDAQRIDDAYRLLYSRPPSEEESRLGL
                     AFLQGDGTQGGPAALERWQQYAHVLLAASELLYID"
BASE COUNT          369 a          682 c          667 g          390 t
ORIGIN      
        1 cgtgatcgag gcccagcagg tgcaggatcg tggcgtgcag gtcgtgaata tggacgcggt
       61 tctcgaccgc gtgcatgccg acctcgtcgg tcgcaccgta catcaggccg cccttcacgc
      121 cgcccccagc catgaggatc gaaaagcccg tgtgatgatg gtcgcggccc ggcttgccct
      181 tgtcctgctt ttcggcatac ggcgtgcggc cgaactcgcc cccccagacg acgagcgtgt
      241 cttccaacag tccccgctgt ctgagatcgg tcagtaaggc cgttgtcgcc cggtcggcat
      301 cctcgcacag cttgcgatgg cggttgtcgt tgtcggcgtg cgtatcccaa ggctggttat
      361 tgtccttgga gaggtaatag accgagacgt accgcacgcc gtgctcgcac aagcggcggg
      421 ccaggaggca ctgctggccg aagttcgtct ggccgtaggc tgcgcggacc gattccggct
      481 cgcggccgat gtcgaaggcc tcggcagctt cggcctgcat ggaaaaagcg gtctccatcg
      541 cctgaatctg ggcttccagg cccgagtcgc ccggccgctc ggccaaatgc agccgattca
      601 aagcagccaa cagatcgacc tgctcgcgct gctcatcgcg gctgagcgtg ggatgccgca
      661 ggttcgtcac cagcttctcg acctgcatgt cgttcgtatc gacggacatt ccctgatggg
      721 ccgctggcag gaacgaagca gaccacaagt gcgggccgac caccggtcgc cgcggcgcga
      781 gcacgacaaa cgccggcaag ttctgattct cggtccccag gccatagctg acccaggccc
      841 ccagcgacgg ccggatcggc tgctggttgc cggagtgcat caagagcagg cccggctcgt
      901 ggttcggcac atcggtgtgc atcgagcgga tcacgcacaa gtcgtcggca tggcgggcga
      961 ggttcggaaa caactcactc attaccacgc cgctctcgcc gtgcgctgcg aactggaagg
     1021 gagacggaat gaagcctccc ttctttttcc gcatcgtgtc cccttccggc atctgccctt
     1081 cgtgatcctt gagggccggc ttggggtcga aggtatcgac gtgcgacggc ccgccgttca
     1141 tgaacagata aatcactcgc ttggcccgcg gagcgaagtg cggcgatgga tatgccggtt
     1201 cgttggcagc ctgcgccggt gcgccgatca agcccgcggc gtgcatcgcc ccagccaggc
     1261 caatcgtgcc aaagccggca gcactttggg cgagcatttt ccggcgggaa acgagcggca
     1321 agcgattggt cgtcatggtc gtcctcagtc gatatacagc agctcgctgg ccgcgagcag
     1381 cacgtgagca tactgctgcc agcgttccaa agcagcaggc ccgccctgtg tgccgtcacc
     1441 ttgcagaaat gccaggccca atcgcgactc ctcctcgctc ggcggccgcg aatacagcag
     1501 ccgataggcg tcgtcgatcc gctgggcgtc gtccaggctt tcatcctgct gccgcagggc
     1561 cagcgaggct gcctgttcaa tcatgaattg gctgttgagc acgaacatct tctgcaatgg
     1621 cgtggtggtt tcgctacggc ggtcggcgtg gacgttcggg tccgggtagt cgaacaatgc
     1681 cagcatccgg ttgagctcca gccggctggc ctggctgtag atcgtacggc ggcgctcctc
     1741 gggattctgc ggatcgagcg acggcccgcc gattgccgtg tccagccgcc ctgccgccat
     1801 tagcagcgaa tcgcgccact gctcgactgt catccgccgg cgattcatgt gggcaaggag
     1861 gcgattctcc gggtcagcca attgcggatc gcggattgcg gattgcgaat tggacgactg
     1921 gcggtacgcc gacgacaaca cgacctccct tgccagccac ttcaatgacc aaccccggtc
     1981 catgaaccgc acggccagat cgtcgagcaa ttcgggatgc gtgggaaagt cgccgcgcaa
     2041 accgtagttg ctgggcgtcg cgacgagcgg ccggcccata tacagccccc acaggcgatt
     2101 cacaatta
//
LOCUS       DASUGB010001044         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_115303, whole genome shotgun sequence.
ACCESSION   DASUGB010001044 DASUGB010000000
VERSION     DASUGB010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115303"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFV87_19780"
     CDS             complement(<1..308)
                     /locus_tag="VFV87_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106074.1"
                     /translation="MLEQDDGDQQRNHEQRAKRLQPAREFRRAWYLSRSSVRPEPRRE
                     GTHRSGRDGSACQDGWNNRQPAAEHAGLWAEGQERYSQCHHNHVGGNRLPGQPSACSR
                     G"
     gene            complement(363..2180)
                     /locus_tag="VFV87_19785"
     CDS             complement(363..2180)
                     /locus_tag="VFV87_19785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106075.1"
                     /translation="MLVALQKYRVLSTQYLVLSTCLLIGGWLAVIPFKPAFAQSSTAA
                     PLIRLLESGRLPPERQLAVLEMICTRGEAADLAVVVKQLDKPDGLTPAARRKLIELLA
                     DAAVTRKIKPAGDLSPLVALIESESAAKDAALQRGAIRLAAAWKLSEVAPVLRELALS
                     ESTDPALAEAALGGLVAIGGAESRQTIEQVAASGKSAALRALAIASLSRIDTDSAAAA
                     AADVLQVAKPDDNIAPMLDALLGRKGGADKLAAALQARPPSADAAKLALRHMYSVGRS
                     DQALSDVLSKAAGIALDAPPPSQEEVAKIAAEVTAKGDPERGERIFRRGDLSCMKCHA
                     VAQAGGSVGPELSAVGSISPVDYVVNSILNPNLAIKEQFVTRRVLTFDGEVLTGIQID
                     RDDQRLRLKDATGKVIDVPTDDIEREGEGVSLMPQGLTKFLTHQEFLDLASFISQLGK
                     PGPYAIRKTPSIQRWRVLKNPPPEITAEVPNVEIVRELVLDAPADAWTTAYGTVGGSL
                     PLAELARERPAVVWLQGEIDVNSAGPVAVEIASAEKLQVWLDAEPFEGQARVERELAA
                     GKHTLTFRVEISEREDPQLKVEITKPAGSGAQFVVANGM"
BASE COUNT          385 a          825 c          821 g          451 t
ORIGIN      
        1 ccacgcgagc aggcgctcgg ctgacctggc agtcgattgc cgccgacgtg gttgtggtgg
       61 cactggctat agcgctcttg gccctcggcc cagagaccag catgctccgc agcgggctgg
      121 cggttgttcc acccgtcttg gcaagcgctg ccgtcgcgtc cgctgcggtg tgtgccctcg
      181 cgccgtggtt caggccgcac ggagctgcgg gagaggtacc aagcccgccg gaattcacgg
      241 gccggctgga gacgcttcgc gcgctgctcg tgattgcgtt gttgatcgcc gtcgtcctgc
      301 tcgagcacgg cgggccgtat gccaggccgg tgagtccacc cacaacccgc acgagcgccg
      361 cctcacatgc cattggccac gacgaattga gcccccgatc cagccggctt ggtgatctcg
      421 accttgagtt gcggatcttc acgctcggaa atttcgacgc gaaacgtgag cgtgtgcttg
      481 ccggcggcca gctcgcgctc gacgcgcgct tgcccctcga acggctcggc gtcgagccag
      541 acctggagtt tctcggccga ggcaatctcg acagctaccg gccccgctga gttgacgtcg
      601 atttcacctt gcagccagac gaccgccggc cgctcgcgcg cgagctccgc cagcggcagc
      661 gatccgccca ccgtgccgta agcggtcgtc caggcatccg ccggcgcgtc cagcaccagc
      721 tcgcggacga tctcgacgtt cgggacttcg gccgtgattt cgggcggcgg attcttcagg
      781 acgcgccagc gctgaatgct gggcgtcttg cggatggcat aggggcccgg cttgccgagc
      841 tgcgaaatga agcttgccag gtcgaggaat tcctggtgcg tcaggaattt cgtcagccct
      901 tgcggcatga gcgagactcc ttcgccttcc cgctcgatgt cgtccgttgg cacgtcgatc
      961 actttgcccg tcgcgtcctt cagccgcagc cgctgatcgt cgcggtcgat ctggatgccg
     1021 gtcagcacct cgccatcgaa cgtgagcacg cggcgggtga cgaactgctc cttgatcgcc
     1081 aggttcggat tgagaatcga gttgacgacg taatcgacgg gcgagatgga gccgacggcc
     1141 gacagctccg gcccgaccga gccgccggct tgggcgacgg cgtggcactt catacacgag
     1201 aggtcgccgc ggcggaaaat gcgctcgccg cgctccgggt cgcccttggc ggttacttcg
     1261 gcagcgattt tggccacttc ttcctgtgag gggggcgggg catcgagggc gatgccggcc
     1321 gccttgctca ggacgtcgga caaggcctgg tcgctgcggc ccaccgagta catgtgccgc
     1381 aaggcgagct tggcggcatc ggccgaaggc ggcctggcct gcagggcagc agcgagcttg
     1441 tcagcgccac ccttgcggcc gagcagggcg tcgagcatcg gggcgatgtt gtcgtcgggc
     1501 ttggcgactt gcagcacatc cgctgcagcg gctgcggcgg agtcggtatc gatccgcgaa
     1561 aggctggcga tggcgagtgc ccgcaaggcg gccgatttgc cgctggccgc gacctgctcg
     1621 atcgtctggc ggctttcggc gccgccgatg gcgaccaggc cgcccagcgc ggcctccgcc
     1681 agggccgggt cggttgattc actgagcgcg agctcgcgca ataccggcgc gacctcgctg
     1741 agcttccagg cggcagccag gcggattgcg ccccgctgga gggcggcgtc cttggcagcc
     1801 gattcgcttt cgatgagggc caccagcggc gacaggtcgc cggcgggttt gattttccgc
     1861 gtaacggccg catccgcgag cagctcgatc agtttccgcc gcgcggccgg cgtcaatccg
     1921 tccggcttgt caagctgctt gacgaccacc gccagatcag ccgcctcgcc gcgcgtgcaa
     1981 atcatctcca gcacggcgag ctgccgctcc ggcggcagcc gaccgctttc cagcagccgg
     2041 atcagcgggg cggcggtgga ggattgcgca aaggcgggct tgaaaggaat gacggcaagc
     2101 caaccgccga tgagcaggca agtgctgaga actaagtact gagtactaag tactcggtac
     2161 ttctgcagcg caacgagcaa tcgttgcggc cgattgcaaa ttgcaaattg gcaattgcaa
     2221 atttgcaatt tgccactgcc gccccaccca ctccgcattc cgcgctccgc actccgcatt
     2281 ctctgactcc tgtttccgga acgccacgga gggcgttccc tacttcttca cccgcttctc
     2341 cagcgtgttc atcgtttcct tgagcgtgta cgccaggtag tcgtcttgat cgtgcaagag
     2401 cgactcgacc gcgacgtcga tcgcctcctg atggtcgaaa aagctcaggg ctcggaccgc
     2461 ttccagccgg acccgcggat ga
//
LOCUS       DASUGB010001045         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_256848, whole genome shotgun sequence.
ACCESSION   DASUGB010001045 DASUGB010000000
VERSION     DASUGB010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256848"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1003
                     /locus_tag="VFV87_19790"
     CDS             245..1003
                     /locus_tag="VFV87_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106076.1"
                     /translation="MNRLSAAPLPPVTATAAARVPRSQLRDVPPSGLTQCLLTPLHYE
                     RNYAYPLLVWLHSTGGDEREVRRVMPHISLRNYAALGVRGPGSEPSGYSWPQSPDMIA
                     SAEQTILESVSRARLRFNIHPDRIFLAGYEGAGTMALRIALRHPDRFAGAASLNGPFP
                     TGQAPLAMLASARRLKLLSAHCRDSRSYPIERVCRELSLFHCAGMAITLRQYPCEDEL
                     TTQMLHDLDVWLMEQVTGALSGQEHPAAAPADWN"
     gene            1225..2043
                     /locus_tag="VFV87_19795"
     CDS             1225..2043
                     /locus_tag="VFV87_19795"
                     /inference="COORDINATES: protein motif:HMM:NF042726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6655 family protein"
                     /protein_id="HEX5106077.1"
                     /translation="MLALSMAGGVGCGINKSRLATEQLVISDAVDRAVAAVDFSPLSG
                     QKVYFDTKYVDNVNLGAGANVGYVVSSLRQQMLAYDCRLQEKPETADYVVEARVGVLN
                     YDGHEVTYGVPGNTALGTASVLLSNPVPLPAMPELSLGRRNHQAGTAKIGLFAYDRET
                     REPVWQAGVIKASSQARDMWVLGMGPYQQRPTTHRKRGLFSSRKSADELSAADADPIG
                     AFSSPLVFRRAVEKTAADEDDVATASHETPAEPNPIQPAVDAKPVPAAPSATTP"
     gene            2066..3124
                     /locus_tag="VFV87_19800"
     CDS             2066..3124
                     /locus_tag="VFV87_19800"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5106078.1"
                     /translation="MTSATPQRILITRLSAIGDCVHTMPLACAVRDCFPGARIVWAVE
                     RGAATLVATLAEVDRVLIVPKDWLHSPQAIWQLRHELVAEQFDLVLDPQSLLKSSALG
                     WLTGARRRIGFAPPVGREIAPWLNSERVQSRAVHRVERYLELLQPLGIGLPPVRFGLR
                     VAADADALAAEVIARHDCQAGYAVFSPGAGWDSRLWPIERFAEVARRLAERDLVSLVV
                     WGGQRERQWAEQIVGAASGAAILAPQTTLLQLTALIRRARLFVGCDSGPLHLAGAVGT
                     PCVGLFGSTRREATGPYGPGHTLLQAAYDDSGDRKRPGADNWAMRQISTQMVIDACIQ
                     ILAGDRTDSVPDGLDRAA"
BASE COUNT          516 a         1066 c          992 g          556 t
ORIGIN      
        1 cgtcagtagt gcatttcgcc tccttcttcc tcgacggacc aggttccttg gccgcaaccg
       61 agttgccatc acggtctaag tcgatgggct tccaaagcca cgccggatcg cagcgacggc
      121 cgctcccggc ggacagcgcc caccgttttc agcgacgaac gaaatcgtcg tgcgctcaag
      181 cctgaccatc ccgtttgtgc ctgccggttt catcccgccc taacccataa cattcggacc
      241 cgccatgaac cgactctccg ctgcgccgct gcctcctgtc acagcgaccg ccgcggcccg
      301 cgtcccgcgg tcgcaattgc gggacgtgcc gccgtccggc ctgactcagt gcctgctgac
      361 gccgctccat tacgagcgga attacgccta cccgctcttg gtttggttgc acagcacggg
      421 cggcgatgag cgcgaggtgc ggcgcgtcat gccgcacatc agcctgcgga actatgccgc
      481 cctgggcgtc cgcggacccg gtagtgaacc gagcggctat tcgtggccgc agtcaccgga
      541 catgatcgcc agcgccgagc aaaccattct cgaatcggtg agtcgtgccc gcttgcgctt
      601 caacattcat cccgaccgga tcttcctggc cggttatgaa ggggctggca ccatggcgct
      661 gcggattgcg ctgcggcacc cggaccgctt tgctggggca gcctcgctga acggtccctt
      721 ccccacgggc caagcgccgc tagcgatgct ggcaagcgcc cgtcgcctga agttgctatc
      781 ggcccattgt cgcgattcgc gatcgtaccc catcgagcgc gtctgccggg aactgtcgct
      841 cttccactgc gcggggatgg ccatcacgct gcggcaatat ccttgcgagg atgagctgac
      901 gacgcaaatg ctgcacgatc tggatgtctg gttgatggag caggttactg gcgccctcag
      961 cgggcaggaa catccagccg ctgcgcccgc cgattggaac tgagctgtct gcttgcgaca
     1021 acagcacacc tgcgggttgc cgcagatcgc tatcgcgcgc atgcgcagtg atcctggcgg
     1081 cgggcggacg aagctttcga ccgaccaaac tcccattggt cggctgccgc gggctcgcta
     1141 cactcccgcc ggtcttgggg ataggtgcag cgcgtggcag caaacggaat tgcaacagct
     1201 cgaaatcgcg ttgctgcttg gggaatgctt gcgctgtcga tggccggcgg cgttggatgc
     1261 ggcatcaaca agtcgcgcct ggcgaccgag cagctcgtga tctccgatgc cgtcgatcgc
     1321 gcggtagccg ccgtcgactt cagcccgctc tcggggcaga aagtctattt cgacaccaag
     1381 tacgtcgata acgtcaatct cggagcgggc gccaacgtgg gatacgtcgt cagctcgctc
     1441 cggcaacaaa tgctcgccta cgattgccgt ctccaggaaa agccggagac ggccgactac
     1501 gtcgtcgagg ctcgcgtcgg agtattgaac tacgacgggc atgaagtcac gtatggcgtg
     1561 ccgggaaata cggccctggg cacggcctcc gtgctgcttt ccaacccggt gccgctgccg
     1621 gccatgccgg agctttcgct gggccgtcgg aatcaccagg ccgggacggc taaaattggg
     1681 ctattcgcct atgaccgcga gacgcgcgag ccagtctggc aagcgggcgt catcaaggcc
     1741 tccagccagg cgcgcgacat gtgggtgctc ggcatggggc cttatcagca gcggccgacg
     1801 acccaccgaa agcgcgggct cttttcgagc cgcaagtcgg cggacgaatt gtccgcagcc
     1861 gatgccgatc cgatcggcgc gttctccagc ccgctcgtgt ttcgccgtgc cgtggagaag
     1921 accgcggcag acgaggacga cgtggcgacc gcgtctcacg aaacgcctgc cgaaccgaat
     1981 cccatccagc ccgccgtgga cgccaagccg gtccctgcgg cgccctcggc gacaacaccg
     2041 tagttccacc tcggatgaag ccgcgatgac ctccgctacg ccccagcgaa ttctgatcac
     2101 gcggctgagc gccatcggcg actgcgtcca cacgatgccg ctggcctgcg ccgtgcgcga
     2161 ttgctttccg ggcgctcgta tcgtgtgggc agtcgagcgc ggggccgcga cattagtcgc
     2221 cacgctggct gaggtggatc gcgtgctaat cgtgcccaag gactggctcc attcgccgca
     2281 ggcaatctgg cagctccgcc acgaactggt cgccgaacaa ttcgacctgg ttctcgatcc
     2341 gcaatcgctc ctgaaaagct cggctctcgg ctggttgacg ggggctcggc ggcggatcgg
     2401 atttgcgccg cccgttggcc gcgagatcgc accctggctc aatagcgagc gcgtccaaag
     2461 ccgggcagtc catcgtgtcg agcgctatct ggaactcttg caacctctag gaatcggcct
     2521 tccgccagtg cgtttcggct tgcgcgtggc cgccgatgct gatgctctgg ccgccgaagt
     2581 catcgcccgg cacgactgcc aggccggcta cgcggtgttt agccccggcg cgggttggga
     2641 ctcgcggctg tggccgatcg aacgattcgc ggaagtcgcc cgccgcctgg ccgagcgaga
     2701 tcttgtttcc ctggtcgtct ggggcggaca gcgcgagcgc cagtgggcag agcagatcgt
     2761 cggcgctgcc tccggcgctg ccatactcgc accgcaaact acactgctgc agctaacggc
     2821 gctgatccgc cgcgcccggc tgttcgtggg ctgcgattcc ggaccgctgc acctggccgg
     2881 cgccgtcggc acgccctgcg tcggcctgtt cggcagcacc cgccgcgaag cgaccgggcc
     2941 ctacggcccc ggccatactt tgctccaagc ggcctacgac gactccggcg accgcaagcg
     3001 ccccggcgcc gacaactggg cgatgcggca gatcagcacg cagatggtga tcgatgcgtg
     3061 cattcaaatc cttgcaggcg accgaacgga ctcggtacct gacgggctgg atcgcgcggc
     3121 gtaagcgtca
//
LOCUS       DASUGB010001046         6720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_811287, whole genome shotgun sequence.
ACCESSION   DASUGB010001046 DASUGB010000000
VERSION     DASUGB010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811287"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1588)
                     /locus_tag="VFV87_19805"
     CDS             complement(248..1588)
                     /locus_tag="VFV87_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX5106079.1"
                     /translation="MTKPVAELPVPWIQTPLPGPKAAQLIATDELYTSPSYTRVYPLA
                     CERGEGAVIEDVDGNRFLDFTAGIAVCSTGHCHPRVVAAIQQQAGKLLHMSGTDFYYA
                     PQGELAKKLAQIAAGSGAKRIFFTNSGAESVEAAFKLARYHTGRQHMLAFFGAFHGRT
                     LGALSLTGSKVVQRRGFAPLIPMVSHVDYPNCYRCSNGPQDGVCCLDSLNQIHDLFKR
                     TVAPQEVAAIIVEPIQGESGYIVPPPEFHRELRKLCDQHGILLIADEVQSGMGRTGKM
                     FAMQHWGVTPDIVCLAKGIASGMPLGAIIARAEIMDWGPGSHASTFGGNPVSCVAALE
                     TIKLLEEGLMENARTVGEYLKGRLTELMARHKLIGDVRGLGLMIGVELVRDRATKEPA
                     STERDEVVQLAFREGLLLLGCGVNTLRFCPPLVVTKEQCDVAVGILDEVLGKWD"
     gene            1769..3064
                     /locus_tag="VFV87_19810"
     CDS             1769..3064
                     /locus_tag="VFV87_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106080.1"
                     /translation="MTHRQLSMSSVYFVVSLALYASLCTSVLAVEPPVLNWPGWRGPK
                     GDGSSPETNLPTTWNGTTGENVVWKTAIPGTGHSSPIVWGERIFVVSCLPDSGDRVLL
                     SLDRKTGAVLWQQTVLKAPLEKKHTLNSYASGTPATDGRLVYVSFLEPDFASQKERTP
                     GNMVVAAYDFEGNLKWSAKPGRFASTHGYCSSPVLFEDKVIINGDHDGDGYIAALARE
                     SGQEIWRIERPNNTRSYVTPLIRDIAGKTQMLLSGSKCTASYDPRTGKELWRMDGPTE
                     QFVASLVDDGRYVYLTAGFPERHILAIDPSGQGHVEDDKIVWRTKKNCSYVPSPVICG
                     EHFVVAADDGIASCYQAQSGELQWTARLGKHYSASLVTAGGLVYLLADDGIMKVVRPG
                     PELDVVAENPLGEYCYASPALSQGQILVRGEEHLYCIGR"
     gene            complement(3153..4616)
                     /locus_tag="VFV87_19815"
     CDS             complement(3153..4616)
                     /locus_tag="VFV87_19815"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5106081.1"
                     /translation="MNDNRAGRIRPACCELSNMPAAKTSAAYLAPFLAIFGCLIGPPP
                     AARAAEPAAGPNFIVIFADDLGYGDLSCYGHPTIRTPHLDRMAREGMRFTQFYSAAEV
                     CTPSRAGLLTGRLPPRSGMCSNKRRVLFPDSKGGLQAEEVTIAELLKAKGYATACIGK
                     WHLGHLDPFLPTKHGFDSYFGIPYSNDMDRVASAPAGRAAFLDPKIAYWNVPLMRNTE
                     VIERPADQHTLTRRYADEAVRFIQAHKNQPFFLYLPHTMPHVPLFASSEASGASARGL
                     YGDVVEELDASVGKILQSLKDEQLAERTLVFFTSDNGPWLIQGEQGGSAGLLRDGKGS
                     TWEGGMREPAIAWRPGTVPAGTISHELAATLDFLPTFAAQAGADLPAERPLDGYDLTP
                     ALAGGKSPRQEMFYYRGYELMAVRSGPWKAHLQTQAGYGQPKPEIHDPPLLFNLLIDP
                     GENYNVAARHPEVVAKLLSLVEEHSAGMKHAPSQLEL"
     gene            4790..6124
                     /locus_tag="VFV87_19820"
     CDS             4790..6124
                     /locus_tag="VFV87_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5106082.1"
                     /translation="MPAKGTKVDRRRFLGAALSTAGAAAAAIGAPTIIPSSALGRDGA
                     TPPSERVIVGGIGIGNRGTYDLGCFLEQNDVQFAAVCDIKEARRLAVKKRVDELYGNE
                     SCLMFRDFRELLDRSDIDAVLIATGPNWHATMAMNAAKAGKDMYCEKPCTKNIEQSLI
                     LADTIRRTGRVFQVGTQRRNLPHFAFACELARTGKLGKLQRVYAHPRGMLAFPSTWFP
                     AEPEPDKNVVDWDMYLGPAAWRPYNAKLLDGFNFEKGGGMIGGFEKEAGKPPRLGGGV
                     LEWGSHCVDLCQWAVDDRPPPVEYNPPANGQIVCKYDNGVELIFREDGWIPLGSCPVR
                     FEGETGWVEAGDSGKIVLSSPALLAGRTVEEIPNYPATLHVRDFLDCVKTRGKPKGNA
                     AAARNAHIACHAANIGLYLNRQVKYDVVKNEFIGDEQANRMRSEALRESWRL"
     gene            6379..>6720
                     /locus_tag="VFV87_19825"
     CDS             6379..>6720
                     /locus_tag="VFV87_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PBS lyase"
                     /protein_id="HEX5106083.1"
                     /translation="MAFACSSEAAAQLRSEQELIALLQSDAPEADKALACKELSIVGS
                     AAAVPELAKLLSSERLASWARTPLEAMPGAEADEALRKASESLEGKLLVGVINSIGVR
                     RDAAAVELLAGR"
BASE COUNT         1241 a         2045 c         2275 g         1159 t
ORIGIN      
        1 ccaagcacct gcgcgatcgg ctgccccgtc gccgggttga gcgagacgaa ctcctcgccg
       61 ccaggccgat caatccaccg gccggcatag acgccggaat tgatcggctg aattcccaac
      121 cgcgcgagta cgtcgtcaaa catggcttgc ctccccatcg gaaattcagc cacggatgga
      181 acacagatta aacacggatt ccgtcagacc catccgtgtt tgatccgtgt ttcatccgtg
      241 gctcctgtta gtcccacttc cccagcacct catccagaat ccccaccgcc acatcgcact
      301 gctccttggt cacgacgagc ggcgggcaga agcggagggt gttcacgccg cagccgagca
      361 gcagcagccc ttcgcggaag gcgagctgga cgacttcgtc gcgctcggtg ctggcgggct
      421 ctttggtcgc gcggtcgcgg acgagttcca cgccgatcat cagaccgagg ccgcggacgt
      481 caccgatcag cttgtggcgg gccatcagct cggtcaggcg gcccttgagg tactcgccaa
      541 ccgtacgggc gttttccatc agcccttctt ccagtagctt gatcgtttcc agggcggcga
      601 cgcacgagac cgggttgccg ccgaaggtgc tggcgtgcga gccggggccc cagtccatga
      661 tctcggcccg ggcgatgatc gcgccgagcg gcatgccgct cgcgatcccc ttggcgaggc
      721 agacgatgtc gggcgtgact ccccagtgct gcatggcgaa cattttgccg gtgcggccca
      781 tgccggactg gacttcgtcg gcgatcagca ggatgccgtg ctggtcgcac agcttccgca
      841 gctcgcggtg aaactccggc ggcgggacga tgtagccgct ttccccctgg atcggctcga
      901 cgatgatcgc ggccacctct tgcggggcga cggtccgctt gaagaggtcg tgaatctgat
      961 tgagggagtc aaggcagcag acgccgtctt gggggccatt cgagcagcgg tagcagttcg
     1021 gataatccac gtggctcacc atcgggatca acggcgcaaa gccgcggcgc tgcacgacct
     1081 tgctgccggt gagcgagagg gcgccgagcg tgcggccatg gaacgcgccg aagaaggcga
     1141 gcatgtgctg gcggccggtg tggtagcggg cgagcttgaa ggctgcttcg accgattcgg
     1201 ccccgctgtt ggtaaagaag atccgcttgg cgccgctgcc ggcggcgatc tgggcgagct
     1261 ttttcgccag ctcgccctgc ggggcgtagt aaaaatcggt ccccgacatg tgcagcagct
     1321 tgccggcttg ttgctgaatg gcggcgacga cgcgggggtg gcaatggccg gtcgaacaga
     1381 ccgcgatgcc ggcggtgaaa tcgaggaagc ggttgccatc gacgtcttcg atgacggccc
     1441 cctctccccg ctcgcaggcc agcggataga cgcgggtgta cgacggcgac gtgtacagct
     1501 cgtcggtggc gatgagctgc gcggccttcg gcccgggcag gggcgtctga atccagggca
     1561 cgggcagttc ggctacgggc ttggtcatgg cgcggcatcc tgggccagaa tcacatcacg
     1621 ggcgacgtta aaatcgtacg ccaaggggcg aattggtcaa agttcaacgg gaaacgccgc
     1681 ctgaggcagc gaaatggatc gcgacgcgtt agaataaatc ccctcgcctc ctaccagccc
     1741 accctccacg tccctccgaa ctcccgccat gacccaccgc cagctttcca tgtcgtccgt
     1801 gtatttcgtg gtttccctcg ccctctatgc gagtctttgt acgagcgtct tggcggtcga
     1861 gccgccggtg ctgaattggc ctggctggcg ggggccaaag ggcgacggca gcagcccgga
     1921 gacgaatctg ccgacgacgt ggaatggaac gacgggcgag aatgtcgttt ggaagacggc
     1981 gattcccggc acggggcaca gctcgccgat cgtgtggggc gagcggattt tcgtcgtgag
     2041 ctgcctgccg gactccggcg accgcgtgct gctgtcgctc gaccggaaga cgggcgcggt
     2101 gctgtggcag cagacggtcc tcaaagcgcc gcttgagaaa aaacacacgc tgaacagcta
     2161 tgccagcggc acgccggcga ccgacggccg gctcgtctat gtctcgttcc tggagccgga
     2221 cttcgccagc cagaaggaac gcacgccggg caacatggtc gtcgcggctt acgattttga
     2281 gggcaatctg aagtggtcgg caaagccggg acgcttcgcc agcacgcacg gctactgcag
     2341 ctcgccggta ctgttcgagg acaaggtgat catcaacggc gatcacgacg gcgacggata
     2401 tatcgcggca ttggcccgcg agagcggcca ggaaatctgg cggatcgagc ggccgaacaa
     2461 cacgcggagc tacgtcacgc cgctgattcg cgacattgcc ggcaagacgc aaatgctcct
     2521 ctccggcagc aagtgtaccg ccagctacga cccgcggacc ggcaaggaat tgtggcggat
     2581 ggacggcccg accgagcagt tcgtggcgtc gctggtcgat gacgggcgct atgtctatct
     2641 cacggcgggc ttccctgagc ggcacatcct ggcgatcgat ccgagcggcc aggggcacgt
     2701 cgaggacgac aagatcgtct ggcggacgaa gaaaaactgc tcgtacgtcc cttcgccggt
     2761 gatctgcggc gagcactttg tcgtcgcagc agacgacggc atcgcctcgt gctaccaggc
     2821 ccaaagcggc gaattgcagt ggaccgcccg gctggggaag cactatagcg cttcactcgt
     2881 gacagccggc ggcctggtct atcttctggc cgacgatggg atcatgaaag tcgtccgccc
     2941 cggccccgag ctggatgttg tcgccgagaa tccgctcggc gaatactgct acgcctcgcc
     3001 cgccctcagc caggggcaga tcttggttcg gggtgaagag cacctgtatt gcattggcag
     3061 gtgacgtagc tgccccccgc ggcgaccagg gtccgcaatg tcgcgagcgc ggctcaggca
     3121 tctccgctaa accagtgata gcgctgcttg ggtcagagtt cgagctgcga cggggcatgc
     3181 ttcatgccgg cgctgtgttc ttcgaccagc gacagcaatt tggcgacgac ttccgggtgc
     3241 ctggcggcga cgttgtagtt ttcgccggga tcgatgagga ggttgaaaag gagcggcggg
     3301 tcgtggattt cgggcttggg ctggccgtag ccggcctggg tctggaggtg agccttccag
     3361 ggtccgctgc ggacggccat cagctcgtag ccgcggtagt agaacatttc ctggcgtggg
     3421 cttttgccgc cggccagggc gggagtgagg tcgtagccgt cgagcgggcg ctcggcgggc
     3481 aggtccgcgc cggcttgagc tgcgaacgtg gggagaaaat cgagcgtggc ggccagctcg
     3541 tggctgatcg tgccggccgg gactgtgccg ggacgccagg caatcgccgg ctcgcgcatg
     3601 ccgccttccc aggtggagcc tttgccgtcg cgcaacaggc ccgcgctgcc cccctgctcg
     3661 ccctggataa gccaggggcc gttgtcgctg gtgaagaaga cgagcgtccg ctcggcgagc
     3721 tgttcatcct tcagcgattg caggattttg ccgacgctgg cgtccagctc ctcgaccacg
     3781 tcaccataca ggccgcgggc gctggcgccg ctggcctcgc tcgacgcgaa gagcggcacg
     3841 tgcggcatgg tgtgcgggag atagaggaag aacggctggt ttttgtgagc ctggatgaag
     3901 cgcaccgctt cgtcggcata gcggcgggtg agcgtgtgct ggtcggcggg ccgctcgatc
     3961 acttccgtgt tccgcattag cggcacattc cagtaggcaa ttttcggatc gagaaacgca
     4021 gcccggcccg cgggtgcgga ggcgacgcga tccatgtcgt tggaatatgg aatgccgaaa
     4081 taggaatcga agccatgctt cgtcggcaag aagggatcga ggtggccgag atgccatttg
     4141 ccgatgcaag cggtggcata gcctttggct ttgagcaact cggcgatcgt gacttcctcg
     4201 gcctgcaagc cgcccttcga atcggggaag agcacgcgac gcttgttgct gcacatgccg
     4261 gagcgcggcg gcaggcggcc ggtgagcagc ccggctcggc tcggcgtgca gacctcggct
     4321 gccgaataga actgcgtaaa gcgcatcccc tcgcgggcca tccggtcgag gtgcggcgtc
     4381 cgaatcgtcg gatggccata gcacgacagg tcgccatagc cgaggtcgtc ggcgaagatg
     4441 acgatgaaat tgggtccagc cgccggctcg gctgcacggg cggccggggg cgggccgatc
     4501 aggcagccaa aaatcgcgag aaagggggcc aggtaagcag cggaggtctt cgcagcaggc
     4561 atgttcgata gctcgcagca agcagggcgg attcgacccg cgcgattgtc gttcatcacc
     4621 atgcggcagt caaacattcg ccggcggcca gcacggtgtg ggtgtggaaa cccgattgcc
     4681 ggaggagtat gctgtcggca ttctgcggcc gcttccgcgg cccacgggca atccgaattt
     4741 acggcaatcc agttttccag caaccgagtt ctgcggcggg agagcagcta tgccagcgaa
     4801 gggtacgaag gtcgatcgac ggcggttttt gggtgcggct ctttccacag cgggcgccgc
     4861 ggcggcggcg attggtgcgc cgacgatcat tcccagctcg gccctggggc gcgacggtgc
     4921 gaccccgccc agcgagcggg tgatcgtggg cggcatcggc atcggcaacc gggggacgta
     4981 cgacctgggc tgcttcctgg agcagaacga cgtgcagttc gccgcggtct gcgacatcaa
     5041 ggaggctcgc cgcctggccg tcaagaagcg cgtggacgag ctttacggca acgaaagttg
     5101 cctcatgttc cgcgatttcc gcgagctgct cgatcgcagc gacatcgacg ccgtgctgat
     5161 cgccaccggt cccaactggc acgccacgat ggccatgaac gccgccaaag ccggcaaaga
     5221 catgtattgc gagaagccgt gtacgaagaa catcgagcag agcctgattc tggccgacac
     5281 catccgccgc accggccggg tgtttcaggt tgggacgcag cggcggaacc taccgcactt
     5341 tgccttcgcc tgcgagctgg cccgcaccgg caagctgggc aagctgcagc gggtctatgc
     5401 ccatccgcgg gggatgctcg ccttccccag cacctggttc ccggctgagc ccgagccgga
     5461 caagaacgtc gtcgattggg atatgtatct cggccccgcg gcctggcggc cgtacaacgc
     5521 aaagctcctc gacggcttca atttcgaaaa agggggtggc atgatcggcg gcttcgagaa
     5581 ggaagccggc aagccgccgc ggctgggggg cggcgtgctg gagtggggct cgcactgcgt
     5641 cgatctgtgc cagtgggcgg tcgatgaccg tccaccgccc gtcgaataca atccgcccgc
     5701 caacggacaa atcgtgtgca aatacgacaa cggcgtcgag ctgatctttc gcgaggacgg
     5761 ctggattccg ctcggctcgt gccccgtgcg gttcgaaggc gaaaccggct gggtcgaagc
     5821 gggcgacagc gggaagatcg tgctcagctc gccggccttg ctggccgggc gaacggtgga
     5881 ggagattccc aactatccag ccacgttgca cgtccgcgac ttcctcgact gcgtgaagac
     5941 ccgcggcaag ccgaagggaa acgccgccgc ggcccgcaac gcccacatcg cctgccacgc
     6001 ggcgaacatc ggcctgtatc tcaaccggca ggtcaagtac gacgtggtca agaacgaatt
     6061 catcggcgac gagcaggcca accgcatgcg gtccgaggcg ctgcgggaat cgtggcggtt
     6121 gtaaagcagc aactcgatag tggaagacgt tccggcaatt gaaaaatgca aattgaaaag
     6181 tgcaaattgc aaattgtaag tgagtgatcg agaagaaacc aattcgcaat ttgcaattac
     6241 cactttgcaa tttgcaatcg cgtggcagac gcgactgcca cgcccaagaa aaaaaagaaa
     6301 caatccaaaa cggggaaccg tatcatgcat cagacgtggc gactttcaac gacattgttt
     6361 ggtatcgccc tcatcgcgat ggctttcgct tgcagcagcg aagctgcggc tcaattgcgc
     6421 agcgagcagg agctgatcgc tctgctgcaa tccgatgcac ccgaagccga caaggccctg
     6481 gcgtgcaagg aactctccat cgtgggaagt gcagccgcgg tgccagagct ggcgaagctg
     6541 ctctctagcg agcggttggc ttcgtgggcg cggacgccgc tggaagcgat gccgggagcc
     6601 gaagccgacg aggcgctgcg gaaggccagc gaatcgctgg agggcaagct actcgtgggc
     6661 gtaatcaact cgattggcgt ccggcgcgat gcggctgccg tcgagctgtt ggcgggccgc
//
LOCUS       DASUGB010001047         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_811391, whole genome shotgun sequence.
ACCESSION   DASUGB010001047 DASUGB010000000
VERSION     DASUGB010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811391"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFV87_19830"
     CDS             <1..353
                     /locus_tag="VFV87_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5106084.1"
                     /translation="IDREEIKVHQSPVDLLIREIGHTHSLIMFPEGGRNPGEEMQEFK
                     SGLYYLSKKRPDLELMPVFIDNLNRVLPRGEFLPVPLLSCITVGSPIWLEPGEPKANF
                     LHRAREAVRRLKDV"
     gene            356..1480
                     /locus_tag="VFV87_19835"
     CDS             356..1480
                     /locus_tag="VFV87_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5106085.1"
                     /translation="MPPALLSSDALLLAQFGRNIRLDLRTNVLLFSVMAILLIAYIVG
                     RFLRRQPESTANPAVVRTFNLRVRAWAMMFAILIAGFLFGYVATVVLFALVSFWALRE
                     FITMTPTRRGDHRTLFWTFFLFTPLQYFLIGVGHEFYGFYTIMIPVYASLFIPARIAI
                     SGDPKRFLERVAKIQAALLICVYALSHAPAMLDLELQTADGQPWVLGAPEEPAKADAE
                     EPAPPPAIGAAEPPNVEEQLGSNAGLLFFFVLLVQLNDVFQYMWSQLLGRHVIAPQIN
                     ASRTWEGFVGGVLSTMLAGMLLYWATPFGFWAGPIAGVIAVAGFAGSMTMSGIKRDRG
                     VKDYGTLVSGHAGVLDRIDSICFAAPVFFHITRFFFSNIG"
     gene            complement(1610..1936)
                     /locus_tag="VFV87_19840"
     CDS             complement(1610..1936)
                     /locus_tag="VFV87_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106086.1"
                     /translation="MKKLFVLAALCGLGLFMIGCGEPAANPTPESTPSVSTDSGMPEE
                     GGTEAPAVEGTEGTTPAPEGAEGTTTPAPEGAEGTTPAPGGAEGAAPAPEGAAAPAPE
                     GEAKPE"
     gene            2243..>2759
                     /locus_tag="VFV87_19845"
     CDS             2243..>2759
                     /locus_tag="VFV87_19845"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX5106087.1"
                     /translation="MPDPAQNPPPGWHTLTASEVLRELGTAGGGLTAAEARERLVRHG
                     PNELQSGQRISAWRILLEQFKNVLLLILIIATGLSIATGHGTEAVVIGVIVFFAVGLG
                     FFQEYRAERAMEALQEMAAPTATTLRDGEETQIPARDLVPGDLILLKAGDKVPADCRL
                     LEIANLQSDEAA"
BASE COUNT          456 a          881 c          883 g          539 t
ORIGIN      
        1 tcatcgaccg cgaggagatc aaggttcacc agagcccggt cgatctgttg atccgcgaaa
       61 tcggccacac gcactcgctg atcatgtttc ccgaaggggg ccgcaacccg ggcgaggaaa
      121 tgcaggagtt caaatcgggg ctgtattacc tgagcaagaa gcggcccgac ctggaactga
      181 tgccggtctt tattgataat ctcaaccgcg tgctgccgcg gggcgaattc ctccccgtgc
      241 cgcttttaag ctgcattacg gtcggctcgc cgatctggct ggagcccgga gagcccaagg
      301 ccaatttcct ccaccgcgcc cgcgaagccg tgcggcgact caaggacgtg tgacgatgcc
      361 gcccgcactc ctctcgtcgg acgctttgct cttggcccag tttgggcgga acatccgcct
      421 ggacttgcgg accaacgtcc tgctgttttc ggtgatggcg atcctcttga tcgcctacat
      481 cgtcggccgc tttctccgcc gccagccgga gagcacggcc aacccggcgg ttgtccgcac
      541 gttcaacttg cgggtgcggg cctgggcgat gatgttcgcg atcctgatcg cgggcttttt
      601 gttcggctac gtggcgacgg tggtgctgtt cgccctcgtt tcgttctggg cgctgcgcga
      661 attcatcacg atgacgccga cccgccgcgg cgaccatcgc acgctgttct ggacgttctt
      721 cctcttcacg ccgctgcagt atttcctgat cggggtcggc cacgagttct atggcttcta
      781 cacgatcatg atcccggtct atgcgtcgct cttcattccg gctcggatcg ccatttccgg
      841 cgatccgaag cggtttctgg agcgggtggc gaagattcag gcggccttac tgatctgcgt
      901 ctatgccctc agccacgcgc cggcgatgtt ggatctggaa ctgcagacgg ctgacggcca
      961 gccctgggtg ctgggcgcgc cggaggagcc ggcgaaggcg gacgcggagg agccggcgcc
     1021 gccccccgca atcggcgcag ccgagccgcc taatgtggag gagcaactcg gcagcaacgc
     1081 cggcttgttg ttcttcttcg tgctgctggt gcagctcaac gatgtgtttc aatacatgtg
     1141 gagccaactg ctgggccgcc acgtcatcgc cccgcagatc aatgccagcc gcacctggga
     1201 aggcttcgtg ggaggcgtcc tgagcacgat gctggccggg atgctgctgt attgggcgac
     1261 gccgttcggc ttctgggccg ggccgatcgc gggcgtcatt gccgtcgccg gcttcgctgg
     1321 gagcatgacg atgagcggca tcaagcgcga ccgcggcgtg aaggactatg gcacgctcgt
     1381 cagcggccac gccggcgtcc tcgatcggat cgattcgatc tgcttcgccg cccccgtgtt
     1441 cttccacatc acgcggttct tcttctcgaa tattgggtga gcgatgagcg ggtgagcgct
     1501 gaaactggcc gaacaagaaa tgagtgcggg gagaatgtct caaggatgag tcacctcccc
     1561 gcaatggttt tcgattcagt ccggatggca cccagtgcca gtcggcaggt tattcgggct
     1621 tcgcttcgcc ttccggagcc ggggcggccg cgccttccgg agccggggcc gcgccttccg
     1681 cacccccagg agcaggcgtc gtgccttcgg cgccctcggg agcgggtgtc gtcgtgcctt
     1741 cggcaccctc gggagcgggc gtcgtgcctt cggtgccttc cacagccgga gcctccgtgc
     1801 cgccttcctc aggcatgccc gagtcggtgg agaccgacgg ggtcgactcc ggcgtcgggt
     1861 tggcagcggg ttcgccacag ccgatcatga acaatccgag gccgcacagg gcggccagca
     1921 caaacaactt cttcatggct ttgtttcctc gtaagtgaaa gcctgacaaa gggatacgga
     1981 cagcaggaca gtcctgcatt gtcgttgaat ctaccccaat agagcctgca gctaccgggc
     2041 ctattcccgg aatttccgcc gagcgccggg aaatgtgaaa aatatcgaac ctcccggaat
     2101 agggggcgtc gcaggcttca gggaaatcta ggtcgctggt ggccgttttg ccggcaaggg
     2161 atagcgtgtg gcgctgcccg aatcacccgc acttgcgaag aatctggaat aatccggctc
     2221 catcgcgctg cgggagtgct gtatgcctga ccctgcacaa aatccaccgc ctggctggca
     2281 cacgctgact gcctcggaag tcctgcgcga actgggcacg gcaggaggcg ggctgaccgc
     2341 cgccgaggcc cgcgagcggc tcgtgcggca cggccccaac gagttgcagt cgggccagcg
     2401 gatctcggcc tggagaatcc tgctggagca atttaaaaac gtcctcttgc tgatcctgat
     2461 catcgcgacg ggactctcga tcgccaccgg ccacggcacc gaggcggtcg tcattggcgt
     2521 catcgttttc ttcgcggtcg gtttgggctt cttccaggag taccgggccg agcgggcgat
     2581 ggaagcgctc caggaaatgg ccgcgccgac ggcgaccacg cttcgcgacg gcgaggagac
     2641 gcagatcccc gcccgcgatc tggttcccgg cgacttgatc ctgctcaaag cgggcgacaa
     2701 ggtccccgcc gactgccgcc tcctcgagat cgccaacctg cagtccgacg aagccgcgc
//
LOCUS       DASUGB010001048         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_951286, whole genome shotgun sequence.
ACCESSION   DASUGB010001048 DASUGB010000000
VERSION     DASUGB010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951286"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /gene="katG"
                     /locus_tag="VFV87_19850"
     CDS             <1..1726
                     /gene="katG"
                     /locus_tag="VFV87_19850"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX5106088.1"
                     /translation="VLTGNVALESMGFKTFGFAGGRKDVWEPEQVNWGSEATWLGDER
                     YSGDHQLANPLGAVQMGLIYVNPEGPNQNPDPIAAARDIRETFGRMAMNDEETVALIV
                     GGHSFGKTHGAAPADNVGVDPEAAGLEEQGFGWRNKFGKGKAGDTITSGLEVTWTTTP
                     TKWSNNFLENLFGFEWELTKSPAGANQWVAKNATAIIPDPFDKSKKRLPTMLTTDLAL
                     RMDPGFEKICRRFLKNPAELADAFARAWFKLTHRDMGPRARYLGPEIPKEELIWQDPV
                     PAVDHKLIDAADIAALKGKILATGLPIGQLVATAWASASTFRGGDKRGGANGARIRLA
                     PQKDWEVNQPKQLAEVLKKLEGIQGEFNKAATGGKKVSLADLIVLAGCAAVEQGAKNA
                     GVTVTVPFSPGRTDASQDQTDVESFAIMEPAADGFRNYQKKQYAVTGEELLLDRAQLL
                     TLTAPEMTVLIGGLRVLGANYNQTRLGVFTQREGQLTNDFFVNLLDMSTEWKPTTKET
                     DLFEGRDRKTGQTRWTATRVDLIFGSNAQLRALAEVYACSDSQEKFVKDFVAAWKKVM
                     NLDRFDLG"
     gene            1737..2237
                     /locus_tag="VFV87_19855"
     CDS             1737..2237
                     /locus_tag="VFV87_19855"
                     /inference="COORDINATES: protein motif:HMM:NF025789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HEX5106089.1"
                     /translation="MFELPDSIRPTFIAAGWYPGRRVGVSSAVPPDHPAADIIATFGG
                     LTVARLKGSMGEECGLDDLAFHELAPDPSVLNIWADLLGTTLVGVAEIHYGHGEWYVA
                     LDGRVFGCSRIHDAFWFAGSSFAEAAERSLFGMRVRPLLRPHQRSVTLYGVEFVADSP
                     EVYQYR"
BASE COUNT          549 a          857 c          911 g          469 t
ORIGIN      
        1 ggtcctcacc ggcaacgtcg ccctggagtc catggggttc aagaccttcg gcttcgcggg
       61 tgggcgcaag gatgtgtggg agcccgagca ggtcaactgg ggatcagaag ccacgtggct
      121 cggcgacgag cgctacagcg gcgaccacca gctcgcgaat cctctcggcg cggtccagat
      181 gggcttgatc tacgtgaatc cggaagggcc gaatcagaac cccgatccga tcgccgcggc
      241 acgggacatt cgcgagacgt tcggccgcat ggcgatgaat gacgaggaga ccgtcgccct
      301 catcgtcggc ggccattcgt tcggcaagac gcacggcgcg gccccggccg acaacgtcgg
      361 cgtcgatccc gaagcggccg gcctggagga gcagggcttc ggctggcgga acaaattcgg
      421 caaaggcaaa gccggcgaca cgatcaccag cggcctggaa gtcacctgga ccaccacgcc
      481 gaccaagtgg agcaacaatt ttctggagaa cctgtttggc tttgagtggg agctgaccaa
      541 gagcccggcc ggggccaacc agtgggtggc gaagaacgcg acggcgatca ttcccgaccc
      601 gttcgacaag tcgaagaagc gcctgccgac gatgctcacc accgacctgg ccctgcggat
      661 ggacccgggc ttcgagaaga tctgccggcg gttcctgaag aacccggctg agctcgccga
      721 cgccttcgcc cgggcctggt tcaagctgac ccaccgcgac atgggcccgc gagcccgcta
      781 tctcggcccg gagattccga aggaagagtt gatctggcag gacccagttc ccgcggtcga
      841 tcacaagctg atcgacgcgg cggatattgc cgcgctaaag ggcaagatcc tggctaccgg
      901 cctgccgatt ggtcagctcg tggcgaccgc ctgggcatcg gcttcgacgt tccgcggcgg
      961 cgacaagcgc ggcggcgcga acggcgcccg catccgcctc gccccgcaga aggactggga
     1021 ggtcaatcag ccgaagcaac tggccgaggt gctgaagaag ctggaaggca ttcagggcga
     1081 gttcaacaag gccgccaccg gcggcaagaa ggtctcgctg gccgacctga tcgtgctggc
     1141 gggctgcgca gcggtcgagc agggggcgaa gaacgccggc gtcacggtca cggtgccctt
     1201 ctcgcccggc cggaccgacg cctcgcagga ccagaccgac gtggaatcgt tcgccatcat
     1261 ggagcccgca gccgacggct tccgcaacta ccagaagaag cagtacgccg tcacgggcga
     1321 ggagctgctc ttagaccggg cgcagctcct gacgctgacc gcgccggaaa tgaccgtgct
     1381 gatcggcggc ctccgcgtcc tgggtgcaaa ctacaaccaa accaggctcg gcgtcttcac
     1441 ccagcgcgaa ggccaactca ccaacgactt cttcgtcaac ctgctcgata tgagcaccga
     1501 gtggaagccg acgaccaagg aaaccgatct cttcgaaggc cgcgatcgca agaccggcca
     1561 gacccgctgg accgccaccc gcgtcgatct catcttcggc tccaacgccc agctccgcgc
     1621 cctcgccgaa gtttacgcct gctccgattc gcaggagaag ttcgtcaaag acttcgtcgc
     1681 cgcctggaag aaggtgatga atctggatcg gtttgatttg gggtgagcac atgccgatgt
     1741 ttgagctgcc ggattccatt cgtccgacgt ttatcgcggc tggatggtac ccgggtcggc
     1801 gcgttggcgt ttcatcggcg gttcctcctg atcatcccgc cgccgacatc atcgctacgt
     1861 ttggcgggct tacagtcgcc cggctcaagg gttcgatggg cgaggaatgt gggctagacg
     1921 accttgcgtt tcacgaactc gctccagacc cctctgtctt gaatatttgg gccgatctgc
     1981 tcggcacgac actggttgga gtggcagaaa ttcattacgg ccacggcgag tggtacgtgg
     2041 cgttggatgg acgcgtgttt ggatgcagcc gcatacatga cgcgttctgg ttcgccggct
     2101 catcgtttgc tgaagcggct gagcgatcgc tgttcggcat gcgcgtccga ccgctgttgc
     2161 ggccgcatca gaggtccgtg acgctgtatg gcgttgaatt cgttgccgac agcccggagg
     2221 tgtaccaata tcgatagggt ccgtcttaca caagcaaaac tcaggcaatc gccgcgcgag
     2281 gcggcaggcc caaacgtccg cggctgggac caccgcgaag cggccgatcg ttcccccgcc
     2341 tcatcgcgaa gcggtcacac agcaaagccc aggggcgccg gtcctccggc gcaccctggg
     2401 aaacacagcc cgctcttcgc cggtcccgcg ttgccacgct gtcttggcgg cgaaacaccg
     2461 ggggcgtgcg atttttggaa ttcctggaag aattcggaaa taaatgaact tccgtgcgca
     2521 tctataggaa tgggtcggaa ggcgcaatgg gctggcctgg cagccaggtc aggggtcacg
     2581 agtcaggagg cggggccggg ggaccagaaa tacgggaaaa aatggttgac aacgcgcaac
     2641 agcgcaacat cctcggacgg ggggtgttgc gcggcgcaac gtattgtcag aaagcaagtt
     2701 gcgaataggt tgcgcagatg gttgggccgc aaaaaagttc ggaaagttgc gccccgattg
     2761 gggtcgaggt tggatgcatt gtgacc
//
LOCUS       DASUGB010001049         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1367305, whole genome shotgun sequence.
ACCESSION   DASUGB010001049 DASUGB010000000
VERSION     DASUGB010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367305"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFV87_19860"
     CDS             complement(<1..928)
                     /locus_tag="VFV87_19860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106090.1"
                     /translation="MSEQPQAETIFGKAIEFGAVGERNAFLDGACHGDPALRQQVDKL
                     VHDYFRAGEFLEQPVAQIASPTLDQPITEMPGTPIGSYKLLQQIGEGGMGVVYMAEQT
                     EPVERRVALKIIKPGMDTRQVIARFEAERQALAMMDHPNIAKVLDAGTIPLRIADFGL
                     RIEEQAAANPQSAIPNPQLSGRPFFVMELVRGVPITQYCDEHHLTPRQRLELFLPICQ
                     AIQHAHQKGIIHRDIKPTNILVAEYDQQAVPKVIDFGVAKAIGSPLTEKTMFTGFGQL
                     VGTLEYMSPEQAKVNQLDIDTRSDIYSLGVLLY"
     gene            complement(1022..1579)
                     /locus_tag="VFV87_19865"
     CDS             complement(1022..1579)
                     /locus_tag="VFV87_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5106091.1"
                     /translation="MTDVTQILSQIERGDPSAADQLLPLVYDELRKLATAKLAQEKPG
                     QTLQATALVHDAYIRLVDIDKAQHWNSRGHFFGAAAEAMRRILVEHARRKEAIKRGGD
                     LERVEIPDLVSSEITESLDFLSLDEALRKLELEHPEKAQLVKLRFFAGCSLEETADFL
                     GVSRATAQRHWAYARAWLFGQLNPE"
     gene            complement(2070..2456)
                     /locus_tag="VFV87_19870"
     CDS             complement(2070..2456)
                     /locus_tag="VFV87_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106092.1"
                     /translation="MNIRDRIKELRRVRAGHLRYPKNWRTHLEEQQNALRGILAEVGY
                     VDALMVRDPSGALGGSSALDKQRQAIVRGRRRPPVRFAEGNRPANVARPFQDENSLLI
                     GPRKPTSREWPPLAKAKNNENQGTGQ"
     gene            complement(2598..>3179)
                     /locus_tag="VFV87_19875"
     CDS             complement(2598..>3179)
                     /locus_tag="VFV87_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106093.1"
                     /translation="FDARDTAHWELMGRIMTAFGSPVVDRFVKDQKIEATVDEIEKYK
                     CNFRKSTERNLREWEDRLAELKRELAAPNLANAEKAKLVKEQAMYEKLVGSLREPRAA
                     EVPEELARGFIVAWKTERELHRVYGGRIIFQQAGPEALDGRRRLFEQAEKNGDIRIDD
                     AGVRHLFYYYANMKHTVVDEKVLERPWFLDGGK"
BASE COUNT          568 a          952 c          973 g          686 t
ORIGIN      
        1 cgtagagcag gacgcccagc gaataaatgt cgctgcgggt gtcgatatcg agctggttca
       61 ccttggcctg ctcgggggac atgtactcca gcgtcccgac aagctgcccg aagccggtga
      121 acatcgtctt ttcggtcagc ggcgagccaa tggctttggc cacgccgaag tcgatcacct
      181 tggggaccgc ctgctggtcg tattcggcca cgaggatgtt cgtcggcttg atgtcgcggt
      241 ggatgattcc cttctgatgg gcgtgctgaa tcgcctggca gatcggcagg aagagctcca
      301 gccgctggcg gggcgtgagg tggtgctcgt cgcaatactg cgtgatcggc acgccgcgga
      361 cgagctccat gacgaagaac ggccggccgg acaattgcgg attggggatt gcggattgcg
      421 gattggcggc agcctgttct tcaatccgca atccgaaatc tgcaatccgc aatggaatcg
      481 ttcccgcatc cagcaccttg gcgatgttgg gatggtccat catcgccagg gcctgccgct
      541 cggcctcgaa ccgggcgatc acttggcggg tgtccatccc cggcttgatg atcttgaggg
      601 ccacccgccg ctcgaccggc tctgtctgct cggccatata gaccacgccc attccccctt
      661 cgccgatctg ctgcagcagc ttgtaggagc cgatgggggt gccgggcatc tcggtgatgg
      721 gctgatcgag ggtgggggaa gcaatctgcg cgaccggttg ttccaagaac tcgcctgcac
      781 gaaagtaatc gtgaacgagc ttgtctacct gttgccgcag agcagggtca ccatggcagg
      841 ctccatcaag gaaggcgttg cgctccccaa ccgccccgaa ttcgatggct ttaccgaaga
      901 tggtttcggc ctgaggttgt tcggacatgg ttcacccttt ccaacatgtc tctctaccta
      961 tcagtgcgta ttccccgaca cctcgtgctc acgtgcggcg aaagtgtttg cagaaattcc
     1021 cctactcggg attcagctgt ccgaatagcc acgcacgggc gtaagcccaa tgacgctggg
     1081 cagttgcgcg agataccccg agaaagtcgg cggtctcttc gagcgagcaa ccggcgaaaa
     1141 accgcaactt aaccagctgc gctttttccg gatgctcaag ctcgagtttc ctcagcgctt
     1201 catcaagcga tagaaaatcg agtgactcgg tgatttcgga ggaaaccaga tcggggattt
     1261 caactctttc aagatcgccg ccacgcttga tcgcctcctt gcgacgggca tgctctacta
     1321 gaatccgacg catggcctca gccgccgccc caaagaagtg tccccggcta ttccagtgct
     1381 gggccttatc gatatccaca agacgaatgt aagcgtcgtg caccagcgcc gtcgcctgca
     1441 gcgtctggcc tggcttctct tgtgcaagct ttgccgtggc cagtttccgc agctcgtcat
     1501 agaccagcgg caagagttga tcggccgcgc ttgggtcacc ccgttcaatc tgcgagagaa
     1561 tctgcgtcac gtcggtcatt tcgaccagga tactgccgag aaacagcagt tgccattggc
     1621 tggcccggcc gcaagtaaag gcgcggaggt gaaccacacc gacgcagatt tggctatttc
     1681 caaactcgca gcggttgcat cggccacgcg cgcaacgaag atcttgccag ctgcagaggt
     1741 gtctcatcct gtgggacacc gctgcgagtt cgcgccggcc gcaacttgcg cttgttggtt
     1801 caaccgccgg caggaagggc gatcgcgaac tgcgggaggt cagcttcacc gacggcaatt
     1861 gggcaggccg acggatcggt gaatactgag tggggtcggc ttgcagcagg cgactaataa
     1921 ccgggagttg cggattcgga ttccgaccgc tcaagcgttg ctgcaggttg ttcgcgcgat
     1981 tgactcagtc gtcgtccttg gtgatggcag tagcagcgtc ggcttgttct gctggcggcg
     2041 acttggcgga gggggcggcc gaatcctttt cactgcccgg tgccttggtt ttcattgttt
     2101 ttcgctttgg ctagtggcgg ccactctcgg gaggttggtt tccgcggtcc gatgagtaag
     2161 gaattctcgt cctggaatgg gcgggcgacg ttcgccgggc gattgccttc cgcgaaacgg
     2221 acgggaggcc tgcgccgccc gcgcactatc gcctgacgct gtttgtctag cgccgacgag
     2281 cctcccaacg caccactggg gtcgcggacc atcagcgcat cgacgtagcc gacctcggcc
     2341 aggatgccgc ggagcgcgtt ctgctgctcc tcgaggtgcg tgcgccagtt cttggggtag
     2401 cggaggtggc cggcgcggac gcggcgaagt tctttgatcc gatcgcggat gttcatgtcg
     2461 gcagcggaag cccggtttgg gtttcacgct ggccaacagg atgcggagcc agagtggcga
     2521 gatgtttgtc ctgcccgacg aaccaacaag gtgctgtgaa cgggggcagg cgggcgcgtg
     2581 gcccgattgg gaaacggtca cttccccccg tcaaggaacc acggccgctc caaaaccttc
     2641 tcgtcaacga ccgtatgctt catgttcgcg tagtaataga acaagtgacg cacgccggcg
     2701 tcgtcaatcc ggatgtcgcc attcttttct gcctgttcaa acaagcgacg gcggccgtct
     2761 agcgcctcag gtcccgcctg ctgaaagata atgcgacccc cgtacacgcg gtgcagctcg
     2821 cgttcggtct tccaagctac gatgaaccct cgcgctaact cctcgggcac ctcggccgcg
     2881 cgcggttcgc gcagcgagcc cacgagtttc tcgtacatcg cctgttcttt caccagcttc
     2941 gctttctctg cgttcgccag gttgggagcg gcaagttctc gtttcagttc cgcgagtcgg
     3001 tcttcccact cgcgcaggtt tcgctcggta cttttccgaa agttacactt gtacttttcg
     3061 atttcgtcga ccgtggcttc aatcttttgg tctttgacga agcgatcgac gacggggctg
     3121 ccgaaggccg tcatgatccg ccccatcaac tcccagtgag cggtgtcgcg cgcgtcgaa
//
LOCUS       DASUGB010001050         4329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_951605, whole genome shotgun sequence.
ACCESSION   DASUGB010001050 DASUGB010000000
VERSION     DASUGB010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951605"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFV87_19880"
     CDS             <1..258
                     /locus_tag="VFV87_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106094.1"
                     /translation="IAVNDSAGRPVKGAITLEDQERCWLFRSDRPWSAGRHELVIDTT
                     LEDLAGNRIGRPFEVDELRPIEKQVIPEFYRLPVKIVASSK"
     gene            complement(299..1222)
                     /locus_tag="VFV87_19885"
     CDS             complement(299..1222)
                     /locus_tag="VFV87_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106095.1"
                     /translation="MTRSLWLVLCVVAWAIASPLAFAQVEENKQPAAEAPTESPSDRG
                     PAPADGIPAPAAPPAATKTILVPHISYKTVTVPVVVFKPQVQQKTITVQRAVPEQRQV
                     TRMVPVMEPQVRTFTETIPICRMTYEDVPARIQVMVPHLETREGTRTVCKPVLVQESR
                     TVCRDAGDWQDKTVIDAHGCRQVCRVWVPNIVQEQVPVTVCKMQLTEVPFRFQALVCR
                     PEVKNVVRRVCKPLYETQTREVTCTICVPRLVEKQFIEVVCRYEPAEQVVNCVVPVAV
                     TEQRQIQVPVCTLVQKEVTCPAPSCGPTCCW"
     gene            1368..2804
                     /locus_tag="VFV87_19890"
     CDS             1368..2804
                     /locus_tag="VFV87_19890"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5106096.1"
                     /translation="MKRILCLATAVLIASSGLMLFSLAQPGSGQGDGNGEGLGQRVGK
                     KRASFVTVLDFGAKGDGAADDVAAIQALIDAKASTIRFTAGTYRITRPLVVDLDKVGF
                     TSFVADGTAKLVMAGPGPAIKFVGTHGGTAAPDSVKPEVWDRQRMPVVEGLTIVGDHP
                     EADAIEAAGTMQLTVRGVHIRKVRHGIHLVERNRNVLIDACHIYENSGCGVFLDGVNL
                     HQTNISACHISYCGGGGVVVRGGEVRNVHISGCDIEANMAKDGPPTANILFDCAEGSM
                     AEAAITGCTIQHTKEAPDSANIRILGRGFMKRDGENRPFNCGHVTIGDNVLSDVQTNI
                     HLVGARGVTITGNTFWQGYAHNLLVEDSLQVVVGPNMMERNPLYAYTSEGKNCVIFRG
                     CKDCTLSGLHLHNVIDSEAGLVIDKCDRIHLAGCTIYDCDNFGLLSRDTTNSWMDGCF
                     IRDDREGATSTPVKVIGGSGNKIVDKDT"
     gene            2897..3304
                     /locus_tag="VFV87_19895"
     CDS             2897..3304
                     /locus_tag="VFV87_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106097.1"
                     /translation="MLAYTTEDGSRKMDRLVANDAAQSVEQGKRRLESNEMDAIDAVL
                     LYDARIPLQDRKSDAIIAELRAYFSPDSVAMIAVPYTPKDNGAFRVHRPKVLVWESCE
                     DFDLDAAMESFWQGVDEHEEGAKVWNSHLDQST"
     gene            3341..3721
                     /locus_tag="VFV87_19900"
     CDS             3341..3721
                     /locus_tag="VFV87_19900"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX5106098.1"
                     /translation="MLALIAHDAKKPALVEFSIRHRDVLERHPLVATGSTGRLIGEQT
                     GLKIECVAHGPDGGDLIIGARVALGQVKAVIFFRDPLTAQPHEPDVSALMRVCDVHQV
                     PLATNPATAESIVAWLRDLLEESK"
     gene            complement(3730..>4329)
                     /locus_tag="VFV87_19905"
     CDS             complement(3730..>4329)
                     /locus_tag="VFV87_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106099.1"
                     /translation="EELLGAVNRCWKCGQVFVRRPEADGLPPVRMERAPAAAEPLEAI
                     VLDDGPAGGEATGSATTTAVAASPVEPPVAPRVAPLVQYVPKPIAPPVSTAQVVEARR
                     KGMMAMGGTIAAILLGVFALILSFFRAEAAVIALLGLCMGIWGLYSPRRSWALVGVLL
                     CCLAIGLGVYRGANDLYIYIKNQQPVQIEEATVEDSLSE"
BASE COUNT          892 a         1373 c         1283 g          781 t
ORIGIN      
        1 atcgccgtaa atgattcggc gggaaggcca gtaaaaggcg caatcacgct tgaagatcaa
       61 gagcgctgct ggcttttccg ctccgatcgg ccttggtctg ccggccggca cgagttggtc
      121 atcgacacca ccctcgaaga cctcgcgggc aaccgcatcg gacggccttt tgaggttgat
      181 gagctcaggc ccatcgaaaa acaggtcatt ccggaattct accgtctgcc ggtgaagatc
      241 gtagcttcca gcaagtgaca ccgatagcat ctggactgct tcctggctct gccgtcgctt
      301 accagcagca cgtcggcccg cacgaagggg ccgggcaggt gacttctttt tgcacgagcg
      361 tacaaaccgg aacttggatc tggcgctgct cggtgactgc gacgggaacg acgcagttca
      421 cgacttgctc tgcgggctcg tagcggcaga cgacttctat gaattgcttc tcgaccagcc
      481 gtggcacgca aatcgtgcag gtcacctcgc gggtctgagt ttcgtaaagc ggcttgcaga
      541 cgcggcggac gacgttcttc acttctggcc ggcagaccag ggcctgaaag cgaaacggta
      601 cttcggtcaa ttgcattttg caaacggtca ccggcacttg ctcctgaacg atgttcggaa
      661 cccacacgcg gcagacttgc cggcagccgt gcgcatcgat gaccgttttg tcctgccagt
      721 cgcccgcatc acggcaaacg gtgcggctct cctggacgag aacgggcttg cagactgtgc
      781 gggtcccttc gcgcgtttcc aggtgcggca ccatgacctg gattcgtgcc ggcacgtctt
      841 cgtacgtcat ccggcagatg ggaatcgtct ccgtgaaggt ccgcacctgg ggttccatca
      901 ccggcaccat ccgcgtgacc tgccgctgct cgggcacggc acgctggacg gtgatcgtct
      961 tctgctgcac ctgtggcttg aagacgacga ccggaacggt gaccgtcttg taggagatat
     1021 gcgggacgag gatcgtcttt gtggcggcag gtggtgcggc cggagctgga attccgtcag
     1081 ccggagcagg acctctatcc gagggcgatt cagtcggagc ttcggcagcc ggttgtttgt
     1141 tttcctctac ttgagcgaag gcgagcggac tggcaatcgc ccaagcgacc acacacaata
     1201 cgagccacag ggagcgggtc atcgttggaa actcctaagg tgagacctcc tcaatctcat
     1261 gcgaatctgg gaagcatgag atgtttgagt agtatatgaa gactttgcgg cgtgcaaaac
     1321 acagcgaata cgaaattcac atgccatcct tctgggggaa accagccatg aagcgaatcc
     1381 tctgcctcgc aaccgcagtt ttaatcgcct cttccggact catgttgttc tccctcgccc
     1441 agcccgggag tggccaaggc gacggcaacg gcgaagggct tggccagcgc gttggcaaaa
     1501 agcgggcgag cttcgtaacc gtgctggatt ttggggcaaa aggggacggc gcggccgatg
     1561 atgtggccgc tatccaagcc ctgatcgacg ccaaagccag cacgatccgc ttcacggccg
     1621 gaacgtatcg aatcacccgg ccgctggttg tcgatttgga caaggtcggt ttcacatcgt
     1681 tcgtcgccga tgggacggcg aaactcgtca tggccggccc cggcccggcg atcaagttcg
     1741 ttggcacgca cggtggcacc gctgcgccgg attcggtcaa gcccgaggtc tgggatcgcc
     1801 agcgaatgcc tgtcgtcgaa ggcctgacca tcgtcggcga ccaccctgaa gccgacgcca
     1861 tcgaagccgc cggtacgatg caactcaccg tgcggggcgt tcacatccgc aaggtccgtc
     1921 acggcattca cctggtcgag cgcaaccgca acgtgctcat cgacgcctgc cacatctatg
     1981 aaaacagcgg ctgcggcgtg tttctcgatg gcgtgaacct gcaccaaacg aacatctcgg
     2041 cctgccacat cagctattgc ggcggcggag gggtcgtcgt ccgcggcggc gaggtccgca
     2101 acgtccacat cagcggctgc gacatcgaag ccaacatggc caaggatggc ccacccaccg
     2161 ccaacatcct cttcgactgc gccgagggca gcatggccga agcggccatc accggctgca
     2221 caatccagca caccaaagag gctcctgatt cggccaacat ccgcatcctc ggccgcggct
     2281 tcatgaagcg cgacggcgag aaccgaccgt tcaactgcgg ccacgtgaca atcggcgaca
     2341 atgtcctttc cgacgtgcag accaacatcc acctggttgg cgctcgcggc gtcacaatca
     2401 ccggcaacac cttctggcag ggctacgccc acaatctgct cgtcgaagat tcgctgcaag
     2461 tcgtcgtcgg cccgaacatg atggaacgca acccgctcta tgcctacacg tcggaaggca
     2521 agaactgcgt cattttccgc ggctgcaaag actgtacgct cagcggcctg cacctgcaca
     2581 acgtcatcga cagcgaagcg ggcctggtta tcgacaaatg cgaccggatc cacctcgccg
     2641 gctgcacgat ctacgactgc gacaatttcg gcctgctctc gcgcgacacg accaatagct
     2701 ggatggacgg ctgcttcatc cgcgacgacc gcgaaggtgc gacgagcact cccgtcaaag
     2761 tcatcggcgg cagcggcaac aagatcgtgg ataaagacac ctaagcgagg gcgcgatgat
     2821 cactgcgtca aaattggccg gtttctttgc agctcatgca atctggagcg tatcggatgg
     2881 gaatatcctt attccgatgc ttgcctacac gactgaggac ggcagccgaa aaatggatcg
     2941 acttgtagcc aatgatgcgg ctcaatcggt cgagcagggc aaacggaggc tggagtcgaa
     3001 cgaaatggac gccattgatg cagtgttgct ctacgacgcg agaattccgc ttcaggaccg
     3061 caaatcggac gcgatcatcg ccgagctgag ggcttacttc tcgcccgatt cagtagcgat
     3121 gatcgcggtg ccctacacgc cgaaagacaa tggagcgttt cgagttcatc gacccaaggt
     3181 tctcgtgtgg gagtcttgcg aagacttcga cctggatgcg gcgatggagt cgttttggca
     3241 gggcgtcgat gagcacgagg aaggggcgaa ggtctggaat tcccacctcg atcaaagcac
     3301 gtaatcatca accgaacccg aaaggctgga ccttccccac atgctcgccc tcatcgccca
     3361 cgacgcgaaa aagcccgccc tggtcgagtt ctcgatccgc catcgcgacg tcctggagcg
     3421 gcatccgctt gtcgccaccg gcagcacggg ccggctcatc ggcgagcaaa cgggcctgaa
     3481 gatcgaatgt gtcgcccacg gccccgacgg cggcgacctg atcatcggcg cccgcgtcgc
     3541 cctcggccag gtcaaagccg tcatcttctt ccgcgacccg ctcaccgccc agccgcacga
     3601 gcccgatgtg agcgccttga tgcgcgtctg cgacgtgcat caggttccgc tggcgacgaa
     3661 tcccgccacc gccgaatcga tcgtggcctg gctgcgagac ttgctcgaag agagcaaata
     3721 ggcggcacgt tactcgctta gggagtcctc caccgtcgcc tcctcgattt gcaccggctg
     3781 ctggttcttg atgtaaatgt agaggtcgtt ggcgccgcga tagacgccca ggccgattgc
     3841 caggcaacac aaaagcacgc ccacaagcgc ccaactgcgg cgcggcgaat acaggcccca
     3901 aatgcccatg cacagcccca agagggcaat caccgccgct tcggcgcgaa aaaaagaaag
     3961 gatcaaagcg aagacgccca gcaaaatcgc ggcaatcgtg ccacccatcg ccatcatgcc
     4021 tttgcggcgg gcctcgacga cctgagctgt agaaaccggc ggagcaatcg gcttgggaac
     4081 gtattgcacg agtggtgcca cccgcggcgc aacgggaggt tctacgggag acgcggccac
     4141 cgccgtcgtt gtcgctgacc ccgttgcctc gccgcctgcc ggaccgtcgt cgagcacgat
     4201 cgcttccagc ggctcggctg cggcgggcgc gcgctccatc cgcaccggcg gcaagccgtc
     4261 ggcttccggc cgtcggacga acacctgccc gcacttccag cagcggttca ccgcccccaa
     4321 aagctcctc
//
LOCUS       DASUGB010001051         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1089715, whole genome shotgun sequence.
ACCESSION   DASUGB010001051 DASUGB010000000
VERSION     DASUGB010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089715"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFV87_19910"
     CDS             complement(<1..362)
                     /locus_tag="VFV87_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106100.1"
                     /translation="MSAFASSMTIRTCQRSDAASALAIISQAWPEVERQTHLAALWSA
                     LAGGNGQSAIVLSADANEESLAAVAAQILPGRVASVTPPQMKSPTDIGAENTAQRLLE
                     RLNDDLSRAGVELAQALLP"
     gene            516..1817
                     /locus_tag="VFV87_19915"
     CDS             516..1817
                     /locus_tag="VFV87_19915"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5106101.1"
                     /translation="MRLTRILAAAALVLLVTNLGLAQSLLDKVESQLKGVIGGAAGAA
                     APADAAAEKPYLGFVPNEEIKDGKGVLIVSVTPGGPAELGGLKGGDRITAVDGKAVTN
                     LDQFDAIYNTAVVGQKLRMTVDRAGKLQSLTVTLAARPARAEEPGEAPAEPALTDPTP
                     RPALTDPSPRRAPLDPLLPSADPAAPAAPAPLSPIRSKPLEATPAEPAPLDLGTPPAT
                     PEPPVPGVTDDPLPAETSGRPSLGITVVPLTAEARVAYSLPVTRGALIANVRPGSPAD
                     TAGLPVGGTIVAIDGRKIDSADELVAAIRAYRPGQEVEIRYYDGPQLATKTVRLAPAA
                     AGIVAAPGATPPGVGPGAADAPLGLRLGGTDRPLLSRVERAVEGFTNPRGASTVYDPA
                     EMAALRDQVLSLEEEIKSLTERVKLLEAKLSNSAPAANPQP"
     gene            2179..2559
                     /locus_tag="VFV87_19920"
     CDS             2179..2559
                     /locus_tag="VFV87_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106102.1"
                     /translation="MKKVCVTVVLGVVALAFSMQSAEALPPFNKEWVAKYVEGNSNAK
                     FVEAAGTAKCNVCHMGTSKKDHNEYGKAVKKYLTKAKYNEIKEDTEAAKKYIVEGLGK
                     AEGEKSASGKTYGEIIKAGGIPWE"
BASE COUNT          488 a          861 c          827 g          476 t
ORIGIN      
        1 ggcagaagtg cttgagccaa ctcgacgccg gcgcgggaaa ggtcgtcgtt caaacgctca
       61 agcaaacgct gggctgtgtt ttcagcacct atatccgtgg gggatttcat ttgcggcggc
      121 gtcaccgagg cgacgcgtcc cggcaatatc tgcgcagcaa ctgcggccag cgattcttcg
      181 ttggcatccg cggaaaggac gatggccgac tggccattac cacccgccag cgccgaccat
      241 aacgccgcaa gatgcgtctg ccgttcgact tccggccagg cctgcgagat gatcgccagg
      301 gctgatgccg catcggatcg ctggcaagtg cggatcgtca tcgagctagc gaaagccgac
      361 atggtctgcc cacgataccc aaaatcccga tcctctggcg acgccagttg gggcgatgcc
      421 ttataatgcg tggcggacgt ggggcagacc ttcccgtctg ccggcaggca acgattgctt
      481 ggcccacttt gcctcttgcc cgtggagttc gaatcatgcg cctgacacga atcctggcgg
      541 ctgccgccct ggttttgctc gtcactaacc tcggcctggc ccagtcgctg ctcgacaagg
      601 tcgaaagcca gctaaagggc gtaataggcg gcgccgctgg cgccgcagct cccgcagatg
      661 ctgccgccga gaagccgtat ctgggctttg ttcccaacga ggagatcaaa gacggcaagg
      721 gcgtgctcat cgtcagcgta acgcctggcg gcccggccga actcggcggc ctcaaaggag
      781 gcgaccgcat taccgccgtc gatggcaaag ctgtcaccaa cctcgaccag ttcgacgcca
      841 tttacaacac ggcggtcgtc ggccagaagc tgcggatgac cgtcgatcga gctgggaagt
      901 tgcagtcgct caccgtcacg ttggccgcgc gccccgcccg agccgaggag cccggcgaag
      961 cccccgcgga accggccctg accgatccga ctccgcggcc ggcgctgact gatccgagcc
     1021 cgcggcgcgc gccgctcgat ccgttgttgc cctccgccga tcctgcggct cccgccgcgc
     1081 cggccccgct atcgcccatt cgttccaaac cgttggaagc aacgccggca gagcccgcac
     1141 ctctggacct cggcacaccc ccagcgacgc ccgagccgcc cgttccgggc gtgaccgatg
     1201 atccgctgcc tgcggagacg agcggccgcc cgtcgctggg catcaccgtc gtgccgctga
     1261 cagcggaagc ccgcgtcgcg tattccctgc cggtcacccg cggcgcgctg attgcgaacg
     1321 tccggcccgg ctcgccggcg gatacggccg gcctgccggt cggcggaacg atcgtcgcca
     1381 ttgacggacg caagatcgat tcggccgacg aattggttgc ggccattcgc gcctatcgcc
     1441 cggggcaaga agtcgaaatc cgatattatg acggtcctca attggccacg aagaccgtcc
     1501 gccttgctcc cgccgcggca ggcatcgtcg ccgctcccgg cgcaacgccc cccggcgttg
     1561 gtcccggcgc tgcagatgcc ccgcttggct tacggcttgg cgggaccgat cgcccgctgc
     1621 ttagccgcgt ggagcgcgcc gtcgaaggct tcaccaatcc gcgcggcgct tcgaccgtct
     1681 atgacccggc tgaaatggcc gccctccgcg accaggtgct gtcgctggag gaagagatca
     1741 agtcgctcac cgagcgcgtg aagctgctcg aagccaagct cagcaactcc gcccccgccg
     1801 cgaacccgca accgtgattt ttgcaaaaat cgagttgata gcgcgtccgt atgctctcca
     1861 ttcgcggtca tcgcctgggg gaaggcggga ttggtcgttg ctcggtccgt tcggaggcgc
     1921 ccacctcggc gcagtagcga ctaaacatcg gcattcgtgg atgtatgtct cccgcgatcc
     1981 atggcgtggt gatcggcgta gagaagcggg cgcggcgttg attttctcgc ggctttccgc
     2041 ttgacagtga tggatgggca aagtttactg gatggtctgc cctccccact ttcccacctg
     2101 agcggcggct ggcggttggt ccgccggttg cctgtcgttc gcgcctgttg gcatttccca
     2161 ttcctggagg ttttgaggat gaagaaggtt tgcgttaccg tggtgctcgg cgtcgtggct
     2221 ctggcgttca gcatgcagtc agccgaagcg ctgcccccct tcaacaaaga gtgggtggcg
     2281 aagtatgtcg aaggcaattc aaatgccaag ttcgtagaag ccgccggcac agcgaagtgc
     2341 aacgtctgcc acatgggcac cagcaagaag gatcacaacg aatatggcaa ggcggtcaag
     2401 aagtacctga ccaaggccaa gtacaacgag atcaaggaag acaccgaagc cgcgaagaag
     2461 tacatcgtcg aaggcctggg gaaggccgaa ggcgagaaga gcgccagcgg caagacctat
     2521 ggcgagatca tcaaggccgg cggcattcct tgggagtgag ccgctacgat tcagcaacct
     2581 gatcgatctt ggcgggtaag gcccaaatcc ttatccgcct tttttatagg tttattagct
     2641 caaattggtg ga
//
LOCUS       DASUGB010001052         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_812023, whole genome shotgun sequence.
ACCESSION   DASUGB010001052 DASUGB010000000
VERSION     DASUGB010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812023"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VFV87_19925"
     CDS             complement(<1..1088)
                     /locus_tag="VFV87_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX5106103.1"
                     /translation="MNLLYIVLPSAVILLAAYLTYGRVLGRLFQLDPRRVTPATELRD
                     GLDFDPLAPSSLVPQHFSAIAAAGPIVGPILAGLTFGWLPALLWILIGSVLIGGVHDM
                     GALVASIRHKARSIAEVVRDNMSRRSYLLFLTFIWLALIYIIVAFTDVTAQSFIGAAT
                     AENGNVGGGAIATASLLYLVLTILMGVCVRFLKLPMGLATAVFVVLVGGAIVAGPYLP
                     LDVATLAWRPSAGSTSDLYAAAKEGNAAAIATISQARKGWDVALLVYCLLAGIAPVWL
                     ILQPRGQLGGYFLYFALGAGAIGLILGGSHAQYPAFRGWDAALPGSSGAPEALVPMLF
                     ITIACGACSGFHSLIASGTTSKQLRSET"
     gene            complement(1209..>2050)
                     /locus_tag="VFV87_19930"
     CDS             complement(1209..>2050)
                     /locus_tag="VFV87_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106104.1"
                     /translation="PPNSVQVTVRRDATANGPLPLFFAHVLGMKQSHPTATAVAAFEA
                     EKYVVTGFDSDSSGPNGKLLPIAISQDAWNQFLTGGTSPDGVRRDNYTLALTLPNSTV
                     QAPDNVSHVADSTPELGGWFDSGQTPGDFTLVQFDATGKSHSTPSAAGWIRSGPTPYD
                     MGGFGPAGIQATPENPIVLAGSTGMKSSLVDDLQSVVGQPRVLPIYSSYSGNGGNRKF
                     TIVGFAGMSIVKAGGSGNSMFLLCQPTIVIDPTSTTSGSTSTSSTTLTEFVYPGVPLS
                     LVR"
BASE COUNT          393 a          636 c          707 g          314 t
ORIGIN      
        1 tcggtttcgc tgcgtagctg cttggaagtc gtgcccgagg cgatgagcga gtggaatccc
       61 gagcaagccc cgcaagcgat ggtgatgaac agcatcggca ccagggcctc cggcgcgccg
      121 ctcgagccgg gcagcgccgc gtcccagccg cgaaacgcgg gatactgcgc gtggctgccg
      181 ccgaggatca ggccgatggc gcccgcgcct agagcgaaat acaggaagta gccgccgagc
      241 tgtccccgcg gctggagaat caaccacacg ggagcaatgc ccgccaggag gcaatagacg
      301 aggagggcga cgtcccaccc tttgcgggct tgcgagatcg tggcaatggc ggccgcattg
      361 ccttccttgg cggcggcata caggtcgctg gtgctgcccg ctgaggggcg ccaggcgagc
      421 gtggccacgt ccagcggcag atagggcccg gccacaatcg cgccgcctac caacaccacg
      481 aataccgccg tcgccaatcc catcggcagc ttgagaaacc gcacgcacac gcccatcagg
      541 attgtcagca ccaggtacaa gagcgacgcg gtggcaattg ccccgccgcc gacattgccg
      601 ttctcggccg tcgcggcgcc gatgaacgac tgggctgtca cgtcggtgaa tgccacaatg
      661 atgtagatca gggccagcca gatgaacgtc aggaagagga gatacgaccg gcggctcatg
      721 ttgtcgcgga ccacttcggc gatcgagcgg gctttgtggc ggatcgacgc gacgagagcg
      781 cccatgtcgt gaacgccgcc gatcaagacc gacccaatga gaatccacag gagagccggc
      841 agccagccga acgtcagccc cgccaagatc ggcccgacga tcggcccggc ggcggcgatg
      901 gccgaaaagt gctgcggcac gagcgacgag ggggcgagcg gatcgaagtc caatccgtcg
      961 cgcagctccg tggcgggcgt gacccgccgt ggatcgagct gaaacaagcg cccgagcacc
     1021 cggccgtaag tcaggtaggc ggccagcagg atcaccgccg aagggagaac gatgtacagt
     1081 aagttcatgg cgaggccaat tcacgagcga ccacggacgc atggcaggga ccagttcaag
     1141 atacggcatt ctcggccgcg cgtgcaatcg cggaaacgcc ggcgcagggt cctgacttgt
     1201 cgcaaaggtc agcgcaccaa cgacaggggc acgcccggat agacgaactc cgttagcgtc
     1261 gtgctgctgg tgctcgtgct gccgctcgtg gtcgaggtgg ggtcgatcac gatcgtcggc
     1321 tggcacaaga ggaacatgct gttgcccgag ccgccggctt tgacgatgct cattccggcg
     1381 aagcccacga tcgtgaattt ccggttgccg ccgttgccgg aataagacga gtaaatgggc
     1441 agcacgcgcg gttggccgac caccgactgc aaatcgtcaa cgagcgaact tttcattccg
     1501 gtgctgccgg ccaaaacaat cggattctcc ggcgtagcct ggatgcctgc gggcccgaag
     1561 ccgcccatgt catacggggt cggcccgctg cgaatccacc cggcggccga tggggttgag
     1621 tgggacttgc cggtggcgtc gaactggacc agggtgaagt cgccgggcgt ttggccggag
     1681 tcgaaccagc ctcccagctc cggcgtggaa tcggcgacgt gcgaaacgtt gtccggggcc
     1741 tgcacggtcg aattgggcag cgtgagggcc agtgtgtagt tgtcgcgccg cacgccgtcg
     1801 ggcgatgtgc cgccggtcag gaactggttc caggcgtcct ggctaatggc aatcggcagc
     1861 agtttgccgt tggggccgct ggaatcgctg tcgaagcctg tgacaacgta cttctcggcc
     1921 tcgaaggcgg ctacggccgt cgccgtcggg tggctttgtt tcatgcccag cacgtgggcg
     1981 aagaacagcg gcaagggacc gttggccgtg gcatctcgcc gcaccgtgac ttgcacgctg
     2041 ttcggggggc
//
LOCUS       DASUGB010001053         5104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1922743, whole genome shotgun sequence.
ACCESSION   DASUGB010001053 DASUGB010000000
VERSION     DASUGB010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922743"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFV87_19935"
     CDS             complement(<1..322)
                     /locus_tag="VFV87_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106105.1"
                     /translation="MRNFPLIFVILATLSATADEPGRIYENRLTPLQDPPPLLNDLPE
                     FVEPVRELRRFEAPILVNDEGADLSVRAWRFSYNARGIIEMPNRLRADKTAVIMVHPW
                     GIDDG"
     gene            complement(450..1925)
                     /locus_tag="VFV87_19940"
     CDS             complement(450..1925)
                     /locus_tag="VFV87_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106106.1"
                     /translation="MNSHYCHRTRRGFLADLGLGCAGLAMGSLLAQDGIVRADPGTTQ
                     RTFPTAAAGGRAKSVIWIFLSGGVSHLETFDPKPALNKFEVKTYDETGLEKPIKSPYY
                     LSRSRSVVGFDRQVFNKIFPLQVGYQKRGQSGIEVSDWLPHLGECVDDLAIVRSIYTT
                     DNDHAAEFQMHHGRHALDEPQPVIGSWVHYGLGSLNENLPQFVFLGEFKDSRVKKDFD
                     ANYLGPMHTGVQLSLDPANPLPFGSRGKDVLAAEQAHEFALVGELNRLAAGQHPDDEA
                     LRARIQAYELAFRMQMSVPDALSLSAESAETQQLYGIDNEKTAVYGRRLLAARRLAER
                     GVRFTLAYLSDYGEWDSHQKLKENHARSCERVDQPIAGLLKDLKRRGLWDDVLVVWCT
                     EFGRTPGVEAGGMFNAPATGRDHHPHGFSVFFAGAGIKGGVVHGATDELGFHAVLHPH
                     YVTDIHATVLHQLGLDGRRLEIPGRKRLEIDHGQVIREILT"
     gene            complement(1928..4624)
                     /locus_tag="VFV87_19945"
     CDS             complement(1928..4624)
                     /locus_tag="VFV87_19945"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEX5106107.1"
                     /translation="MRHLSILVLLLAAPAARAEEGVDYTRQIKPLLKSHCYACHGALK
                     QQGSLRLDTAAGMLKGGDGGPAIVARSVDASQIVERISSTDESTRMPQEGKLLTAQEV
                     ALLKAWIAAGAHAPSDEQPEPDPKSHWAFQRVVRPGLPLGDEAERSTASSYGQPIDCF
                     LVEKQRELGLEPVGEAPRNVLLRRLYVDLIGLPPSREELNDFLANESPDAYERAVDRL
                     LASPQHGERWARHWMDVWRYSDWYGRRSVPDVMNSYPHVWRWRDWIVKSLNGDSGYDR
                     MVQAMLAADELWPGDQEQVVATGFIVRNWFKWNYDTWMRDQVEHTGKAFLGLTLNCAL
                     CHDHKYDPITQEDYFRFRAIFEPLELRHDRVPGLPDPGPFKKYVYAQSYGPIPHGLVR
                     VFDEKLDAQTFIYSKGDQRLKVEGKPAMPPGVPAALSGTYDLRPISLPATEYYPGLRP
                     FVQEEELAKRRSSVAAAEAAIAAADEKSRPPLEAKVAATTAELASLEARIAADEVRYG
                     VVPGDADAMAKAAAKAEREAKAAGAQAEVAVAEAAEADANAFPDDAKKAAAVKAAADK
                     LAAAKAALTAAQAELTKDDGNYTQLSPVYPDKSTGRRTALAKWITSRENPLTARVAAN
                     HVWRWHFGQPLVPTVANFGRNGKPPTHPELLDWLAAELMDGDWTMKRLSRVLVTSETY
                     RRGSSAEFGMRNAELQLDSTADTPHSALRTPNSIDPDNTCVWRFNPARMEAEVIRDSL
                     LAVAGELDQTIGGQEIDHAQGLTYRRRSIYFAHHGESKMELLELFDAPSPTDCYERST
                     SIRPQQALALSNSVLSREMSRKLAARLTRQVSTDGDFVVAAFEQVLSRSPSIAEAVAA
                     GKFLATQQALYRESAAANDLVKRSRESLVHVLFNHHDFVTVR"
     gene            4781..>5104
                     /locus_tag="VFV87_19950"
     CDS             4781..>5104
                     /locus_tag="VFV87_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase"
                     /protein_id="HEX5106108.1"
                     /translation="MKITRIETHVCHARMRNWIFVKVLTDQVGLFGWGEATLEWHTRA
                     VVGAIEDIAELIVGEDPMRVEHVWQMMFRQHFWHGHGIVRATAMAGVDLALWDILGKV
                     ANLPCS"
BASE COUNT          871 a         1690 c         1567 g          976 t
ORIGIN      
        1 ggccgtcgtc gatgccccac ggatgcacca tgatcacggc cgtcttgtct gcccgcagtc
       61 ggttcggcat ctcgatgatc ccgcgggcgt tgtacgaaaa ccgccaagcc cgcaccgaca
      121 ggtcggctcc ctcatcgttc accagaatcg gggcctcaaa ccgccgcagt tcccgcaccg
      181 gctccacgaa ctcgggaagg tcattcaaca gcggcggcgg atcctggagc ggcgtcaggc
      241 gattctcata gatgcggccc ggttcatcgg ctgtggccga aagggtcgcc aggattacga
      301 aaatcaatgg gaaattgcgc acgttgaggc tccggatagg ccaagtactt agtactcagt
      361 gctcagtact tcttgtttcg cacaatctgc gtcgccgttt ccgatccgcg ttcatccgcg
      421 atatccgcgg caaaaactcc tttccgcgat caagtcagta tttcccgaat cacctgacca
      481 tgatcgatct ccagccgttt gcggccgggg atttcgagcc ggcggccgtc gaggccgagt
      541 tggtgcagca cggtggcgtg gatgtcggtg acgtagtgcg ggtgcaggac cgcgtggaag
      601 ccgagctcgt cggtcgcccc gtgaacaacg ccccctttga tccccgcacc ggcgaagaaa
      661 accgagaagc cgtgcggatg atggtcgcgg ccggtggccg gggcgttgaa catgccgccc
      721 gcctcaacac ccggcgtgcg gccgaattcg gtacaccaga cgaccagcac gtcgtcccac
      781 aggccgcgcc gcttcagatc cttgaggagc ccggcgatcg gctgatcgac gcgttcacag
      841 ctccgggcat gattctcttt gagcttctgg tgcgaatccc actcgccgta gtcgctcaag
      901 taagccagtg tgaaccgcac cccccgctcc gccagccgtc gggccgccaa gagccgccgc
      961 ccataaacgg ctgtcttctc gttgtcgatg ccgtagagct gctgcgtctc ggctgattcg
     1021 gcgctgaggc tgagtgcgtc gggaactgac atctgcatcc tgaaagccag ctcatacgcc
     1081 tgaatccggg cccgcagggc ttcgtcgtcc ggatgctgcc cggcggccag gcggttcagc
     1141 tcgccgacca gggcgaattc atgggcctgc tcggccgcca agacgtcctt gccgcgcgat
     1201 ccaaacggca gcgggttggc tggatcgagc gagagctgca cgccggtgtg catggggccg
     1261 agatagttcg cgtcaaagtc cttcttgact cggctgtcct tgaactcgcc gaggaagacg
     1321 aactgcggca ggttctcgtt cagcgagccg aggccataat gcacccacga gccgatcacc
     1381 ggctgcggct cgtcgagcgc gtggcggccg tggtgcatct ggaactcggc ggcgtggtcg
     1441 ttgtcggtcg tgtatatcga ccgcacgatt gccaggtcat ccacacactc gcccaagtgc
     1501 ggcagccagt cgctgacctc aatgcccgac tgcccccgct tctgatagcc gacctgcagc
     1561 gggaaaatct tgttgaacac ctgccggtca aacccgacaa ccgaccgcga ccgcgagagg
     1621 taataaggcg acttaatggg tttctccagg cccgtttcgt cgtacgtctt cacctcgaac
     1681 ttgttcagcg ccggcttggg atcgaacgtc tccaaatggc tcacgccgcc ggagaggaag
     1741 atccagatga cgcttttcgc ccgaccgccc gccgccgctg tagggaacgt cctctgtgtc
     1801 gttccggggt cggcccgcac gatcccgtcc tgggcgagca gcgatcccat cgccagccct
     1861 gcgcacccca ggccaaggtc ggcgaggaag ccgcgccgcg ttcgatggca gtagtgggaa
     1921 ttcatcgtca gcgcaccgtc acaaaatcat ggtggttgaa caggacatgc accaggctct
     1981 cacggctgcg cttcacgaga tcgtttgccg cagccgattc gcgatacaac gcctgctgcg
     2041 tcgccaggaa tttgccggcg gcgaccgctt cggcgattga gggtgaccgc gaaaggactt
     2101 gctcgaacgc agcgacgacg aagtcgccat ccgtgctgac ttgtcgcgtg agacgcgccg
     2161 ccagcttgcg gctcatttcg cgggacagga cgctgttgga gagggcgagg gcctgctgcg
     2221 gccgaatgga ggtcgagcgc tcgtagcagt cggtcgggct tggagcgtcg aacagctcca
     2281 gcagctccat cttcgactcg ccgtgatggg cgaaataaat gctccgccgc cggtacgtta
     2341 gcccttgagc atggtcgatt tcctggccgc caatcgtctg atcgagctcg ccggcaaccg
     2401 ccagcaggct gtcgcggatc acttccgctt ccatgcgggc tggattgaag cgccagacgc
     2461 aggtgttgtc gggatcgatc gaattcggag tgcggagtgc cgaatgcgga gtgtcggcag
     2521 tcgaatctaa ctgcaattcc gcattccgca ttccgaattc cgcacttgat cctcgccggt
     2581 acgtctcgct cgtcacgagc acgcgactca gccgcttcat cgtccaatcg ccgtccatta
     2641 gctcggccgc gagccaatcg agcaactccg gatgcgtcgg cggcttgcca ttgcggccaa
     2701 aattggcaac ggtcggcacg agcggctgac cgaaatgcca gcgccaaacg tggttcgccg
     2761 cgacgcgggc cgtcagcgga ttttcgcggc tggtgatcca cttcgccagc gccgtccgcc
     2821 ggccagtgct cttgtctgga tagaccgggc tgagttgcgt gtagttgccg tcgtccttgg
     2881 tcagttccgc ctgtgcggca gtcagtgccg ctttggcagc agcgagtttg tccgctgccg
     2941 ctttcacggc ggcagctttc ttggcgtcgt cgggaaatgc attggcatca gcttcagcgg
     3001 cttccgcaac cgcaacctcc gcctgggctc ccgcggcttt cgcctcgcgc tcggccttgg
     3061 cggcggcttt cgccattgcg tcggcatcac caggcacgac accatagcgc acttcatccg
     3121 ccgcgatccg cgcctccagg ctcgccagct ccgccgttgt cgctgccacc ttggcttcca
     3181 gcggaggccg cgatttttca tctgccgcgg caatcgctgc ctccgcggcg gccacagacg
     3241 accggcgttt ggccaactcc tcttcctgca cgaatggccg caggccggga tagtactctg
     3301 tcgccggcaa actgattggc cgcagatcgt acgtcccgct tagcgccgcc ggcacgcccg
     3361 gcggcatcgc cggcttgcct tccaccttca gccgttgatc gcctttgctg tagatgaagg
     3421 tctgggcgtc gagcttctca tcgaacacgc ggaccagccc gtgcgggatc gggccgtacg
     3481 actgggcata aacatacttc ttgaacggcc cgggatcggg cagccccggc acgcgatcgt
     3541 gccgcaattc gagcggctca aagatcgccc gaaagcggaa atagtcctcc tgcgtgatcg
     3601 ggtcgtactt gtggtcgtgg cagagggcac aattgagcgt cagtccgaga aacgccttgc
     3661 cggtgtgctc gacctggtca cgcatccacg tgtcgtagtt ccatttgaac cagttgcgga
     3721 cgatgaagcc cgtcgccacc acctgctcct gatcgcccgg ccacagctca tccgcggcga
     3781 gcatcgcctg caccatccgg tcgtagccgg agtcgccatt cagcgacttc acgatccagt
     3841 cgcgccatcg ccagacgtgc ggatagctgt tcatcacgtc cggcacgctg cggcggccgt
     3901 accagtcgct gtagcgccag acgtccatcc agtgccgggc ccaccgctcg ccatgctgcg
     3961 gcgaggcgag caagcgatcg acggcccgtt cataggcgtc cgggctctca ttggctaaaa
     4021 agtcgtttag ttcctcgcgc gacggcggca atccgatcag atcgacatac agccgccgca
     4081 gcagcacgtt tcgcggtgct tcgcccaccg gctccaggcc cagctcgcgc tgcttctcca
     4141 cgaggaagca atcaatcggc tgcccgtagg acgaagccgt gcttcgttct gcttcgtcgc
     4201 caagcggcag tcccgggcgc accacccgct gaaatgccca gtgcgatttc gggtccggct
     4261 ccggctgctc gtcgctcggc gcgtgcgctc cagccgcaat ccacgccttc agcagcgcga
     4321 cctcctgtgc cgtcagcagc ttcccttcct gcggcatccg cgtcgattcg tcggtggagc
     4381 tgatacgctc gacaatctgg ctcgcgtcca cgctccgggc cacaatcgcc ggcccgccgt
     4441 ctccgccttt cagcatccca gccgcggtgt ccagccgcag actaccctgc tgcttgagcg
     4501 ccccgtggca ggcgtagcaa tgcgacttga gcaggggctt gatctgccgc gtgtaatcga
     4561 cgccctcctc cgcgcgcgcc gcaggcgcgg cgagcaatag gacgaggatc gacaggtgtc
     4621 gcatcgggtg ggctccaatc ggagtcgagc gactgggcag gagagtgcct attacgatac
     4681 caaatccact caccgcgctg caaatggcgt ccgcgttctt cgcgtcatcc gcggttacca
     4741 tctctcccgc tgctatgacc gccagactac gatgagccgg atgaaaatta cccgcatcga
     4801 aacgcacgtc tgccacgccc ggatgcggaa ctggattttc gtgaaggtgc tgaccgatca
     4861 ggttgggctg ttcggctggg gcgaggcgac gctggagtgg cacacgcgcg ccgtcgtcgg
     4921 cgcgatcgag gatattgcgg agttgatcgt cggcgaagac ccaatgcgcg tggagcatgt
     4981 gtggcagatg atgttccgcc aacatttctg gcacggccac ggcatcgtgc gggcgacggc
     5041 gatggctggc gttgacctcg ccctgtggga cattttgggc aaagtcgcga atctgccctg
     5101 cagc
//
LOCUS       DASUGB010001054         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_673115, whole genome shotgun sequence.
ACCESSION   DASUGB010001054 DASUGB010000000
VERSION     DASUGB010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673115"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFV87_19955"
     CDS             complement(<1..1034)
                     /locus_tag="VFV87_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106109.1"
                     /translation="MIHRPRIRMVVFAALLGAASLLAAEDKKRSAGLVAHWPLAGDVQ
                     DHAGGGFHAANRGASLTAEIPGIERGRAAKFDGRGAHLSVPHADGLRLGTGDFTLALW
                     VHTDDAPDDILGDLLSKYDHGKRIGLNLTITHRPGVTHSQANYRQIHFGLDDGRVSQP
                     QWTDHGRPGSAMFIQALAVHDGSLYAGTCEPGKNEAGHVYRLQAQQWIDCGAPDKANA
                     ICSLASFGGKLYAGTGKYRLGGSALAESENPNLGGKVYRYEGEQQWQLCGQLPDVEAT
                     GSLVNFRGELFATSLYRPASFLRYEGSEKWQPLESPFGKRAESLMVFGGQLFASCYDG
                     GKVFRFGGNA"
     gene            complement(1031..1606)
                     /gene="thpR"
                     /locus_tag="VFV87_19960"
     CDS             complement(1031..1606)
                     /gene="thpR"
                     /locus_tag="VFV87_19960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5106110.1"
                     /translation="MARIRTFIAVELGPGVIKRAGDLIDKLRVADAQVGWVKPQHMHL
                     TLKFLGDVSDTETPDICRVVAQAAGQIEPFEIICRGAGAFPDIQRPKTLWIGITDGAE
                     ELKALQAAIEDALKTELGYPKEPRGFHPHLTIGRVKHASAMGRGDLEHLLEKHRDFDA
                     DLAVIDEVVTFASFLERKGPTHEAMGRAELK"
     gene            complement(1698..2600)
                     /locus_tag="VFV87_19965"
     CDS             complement(1698..2600)
                     /locus_tag="VFV87_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5106111.1"
                     /translation="MSLLVVGSVAIDSVQTPTEVRERILGGSASYFSYSASYFGPVRL
                     VGVVGEDFPPQHVELLQSRNIDTSGLHVIPGGKTFFWKGKYQPNMNDRETLEVHLNVF
                     GDFKPSLPDDFRRSDFLFLANSSPVTQMSVLEQATGPKLVVADTMDLWIREQHDELMT
                     LLKRLDGLVMNDSEAKLLTNDENLVAAGHKILKLGPRFVVIKKGEHGAMFFSEHETYV
                     LPAFPTERVIDPTGAGDSFAGGMMGYLASRGNFEPKTLKEAMAYGILVASYTVEDFSL
                     DRLQAISRRDLDTRMGQYRKMLSF"
     gene            2872..>4233
                     /locus_tag="VFV87_19970"
     CDS             2872..>4233
                     /locus_tag="VFV87_19970"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX5106112.1"
                     /translation="MVCPVRLIAVLLCLPAAAMAAPPQYAAMLSSGQRLEGAKLSDWH
                     DVKALPRLDGQPLMEPSNPLRWLRDRTQPLAELPPAYIEFQNGDRLPGVAVDYRSGLE
                     DRYDPVPPHLVVRAAIGFEPPENRPVREIRVATQFIRRIVWQRGSRKTYEPGTVFYRD
                     GRSIAFRAIRFSAGEIHVLLADGDRRIDWSDLAELHLPVVEPWTAWFNQLALLCPTIE
                     TRLVEIETSSGLIATASAARLAMRFEGNSSDSNKWVHALQPAWSLDLLWIPFREIAYY
                     RTFAPREVPLCRISPRQIDGRGGLGAGRAVQNNRNVLGGPLRSKLLDFGFGLGMSGGA
                     IVTIDLPPLGVRSLRAWVCLDRAAGNGGCIRPRILANDAKGQPLWEGPVLVGSEAVAD
                     TGTIGIAGPAAGQKSIVLIVDPVATGRPAGADPLDIRDHANWCDALLELDPAIVQAEI
                     DQRL"
BASE COUNT          730 a         1326 c         1383 g          794 t
ORIGIN      
        1 caggcattgc cgccaaagcg aaagaccttt ccgccgtcgt agcaggaggc gaatagctgg
       61 ccgccaaaga ccatgaggga ctcggcccgc ttgccgaacg gcgattcgag cggctgccac
      121 ttttcgctcc cctcatagcg aaggaagctt gcgggccggt acagcgacgt ggcgaacagc
      181 tcgccgcgaa agttgaccag gctgccggtc gcttcgacgt cgggcagttg cccgcaaagc
      241 tgccactgct gttctccctc gtaacgatac accttgccgc ccaggttcgg attctccgat
      301 tccgccaggg ccgagccgcc caggcgatat ttgccggtgc ctgcatagag cttgccgccg
      361 aacgaggcca gcgagcagat ggcgttcgcc ttgtcgggag cgccgcagtc gatccactgc
      421 tgggcctgca gccgatagac gtgcccggct tcgtttttgc caggctcgca cgtgccggcg
      481 tagaggctgc cgtcgtgaac ggccagagcc tggatgaaca tcgcgctgcc ggggcggccg
      541 tggtcggtcc actggggctg gctgacgcgg ccgtcatcca ggccgaagtg aatctgccga
      601 tagttcgcct gcgaatgcgt cacgccgggg cggtgcgtga tggtgaggtt gagcccgatt
      661 cgcttgccgt ggtcgtactt gctcagcagg tcgcccagga tgtcatccgg cgcgtcgtcg
      721 gtatgcaccc acagcgccag cgtgaaatcg ccggtgccca gccgcaggcc gtcggcgtgc
      781 gggaccgaca aatgagcgcc gcggccatcg aatttggccg cacgtccgcg ctcgattccg
      841 ggaatctcgg cggtcaggct cgcgccgcga ttggcggcat gaaacccacc gcctgcatgg
      901 tcctgcacat cgccggccag cggccagtga gcgacgagtc ccgcgcttcg ttttttgtcc
      961 tccgccgcca gcagcgaagc tgcgcccaag agtgccgcga atacaaccat tcgaatacgc
     1021 ggacgatgga tcatttcaac tccgcccggc ccatggcttc gtgggtcggt cctttgcgtt
     1081 cgagaaagct ggcgaaggtg acgacctcgt cgatcacggc cagatcggcg tcgaagtcgc
     1141 ggtgcttctc gagcagatgt tccaaatcgc cgcggcccat cgccgaggcg tgcttgacgc
     1201 ggccgattgt caaatgcgga tgaaagccgc ggggctcctt cggatagccc agctcagtct
     1261 tcagggcgtc ttcgatcgcg gcctggaggg ccttcagctc ctcggctccg tcggtgatgc
     1321 cgatccagag cgttttgggc cgctgaatgt ctggaaatgc gcccgcgccg cggcagatga
     1381 tctcgaaagg ctcgatctgg ccagcggcct gcgcgacgac gcggcaaatg tcgggcgttt
     1441 cggtgtccga cacgtccccc aggaacttca gcgtcagatg catgtgttgc ggcttgaccc
     1501 agcctacctg cgcgtcggcc acccgcagct tgtcgatcaa atccccggcc cgcttgatca
     1561 cgcctggacc caattcgacg gcgatgaacg ttctgattcg ggccattgcc gggcggctga
     1621 gtggtgagtt gtgagtggta cgaggcagtt tacgtcaacc attcaccact cactactcac
     1681 cactcacttg ccgttaccta gaagctcaac atcttccgat actgccccat ccgcgtgtcg
     1741 aggtccctgc ggctgatggc ttggagccga tcgagactga agtcttccac ggtgtagctg
     1801 gcgacgagga tgccgtaggc catggcttcc ttgagcgtct tgggctcgaa attgccgcgg
     1861 gaagcgaggt agcccatcat cccgccggca aagctgtcgc cggcccccgt ggggtcgatc
     1921 acgcgctcgg tcggaaacgc cggcagcaca tacgtctcgt gctccgagaa gaacatcgcg
     1981 ccatgttcgc cctttttgat cacgacgaat cgcgggccga gcttcagaat tttgtgcccg
     2041 gcggccacaa ggttctcgtc attggtgagc agcttcgcct cgctgtcgtt catcacgagg
     2101 ccgtcgagcc gcttgaggag cgtcatcaac tcgtcgtgct gctcgcgaat ccagaggtcc
     2161 atcgtgtcgg cgacgacgag cttggggccg gtggcctgct ccagcacgct catctgcgtc
     2221 accggcgagc tgttggccag gaacaggaaa tcgctgcggc ggaaatcgtc cggcaggctc
     2281 ggcttgaaat cgccgaagac attgagatgc acctccaacg tctcgcggtc gttcatattt
     2341 ggctggtatt tgcccttcca gaagaaagtc ttgccgccgg gaatcacgtg caggccggag
     2401 gtgtcgatat tgcggctctg gagcagttcg acgtgctgag gcgggaagtc ctcgccgacg
     2461 acgcccacca atcgtacggg accaaagtag ctcgccgagt acgaaaagta cgaagccgag
     2521 ccgccgagaa tccgctcacg gacctcagtg ggcgtctgaa cggaatcgat ggcgacagaa
     2581 ccgacaacga gcagcgacat gatcaaagcc gcgaccagtg gtcgcggttc ccacaccgca
     2641 accgttgggc cgcgggttat cgtttaacgc gcagccgaag gcgtagcgcg tctgagtacc
     2701 taacaaacgg catcgcctgc ggctcgccgt taaacgaggt tgcattatcg agcgatgtcg
     2761 atttcttgca aggatcggta cggtgattgc acgcggcggg ccggcgtaac agaatcaaaa
     2821 cctgcggcca tatccgcgca gggatctgct caaggagacg acgtgccact gatggtttgc
     2881 cctgtgcgac tgatcgcggt gctcttgtgc ctgccggctg ccgcgatggc cgctccgccg
     2941 caatatgccg cgatgctttc cagcggccag cggctggaag gggccaagct gagcgattgg
     3001 cacgacgtga aggcgctgcc gcggctggac ggccagccgc tcatggagcc ctcgaatccg
     3061 ctccgttggc tccgcgaccg cacgcagccg ctggccgaat tgccgccggc ctatatcgaa
     3121 tttcagaatg gcgaccgact gccgggcgtg gcggtcgatt atcgctcggg gctggaagac
     3181 cgttacgatc ctgtgccgcc gcacctggtc gtccgggcgg cgatcggctt tgagccgccg
     3241 gaaaaccggc cggtgcgcga aatccgcgtg gcaacgcaat tcatccgacg aattgtctgg
     3301 cagcgggggt cgcgaaaaac gtacgaaccc ggcaccgtgt tctatcgcga cggccgctcc
     3361 attgcctttc gcgcgatccg cttctcggcc ggcgagattc atgtgctgct ggccgatggc
     3421 gatcgccgca ttgattggag cgacctggcg gaattgcacc tgccggtggt cgagccctgg
     3481 acggcctggt tcaatcaact cgccctgctg tgcccgacga tcgaaacgcg gctggtggaa
     3541 attgaaacgt cgagcggcct gattgcgacg gcatccgcgg cccggctggc gatgcggttc
     3601 gaggggaact catcggactc caataagtgg gtccatgccc tgcagccggc gtggagcctg
     3661 gacctgttat ggattccgtt ccgcgagatc gcgtattacc gcacgtttgc cccgcgtgaa
     3721 gtgccgcttt gtcgcattag tccgcggcag atagacgggc gcggcggcct cggcgcgggg
     3781 cgggcggtgc aaaacaaccg caatgtgctg ggcgggccgc tgcggagcaa gttgctcgat
     3841 tttggctttg gcctcgggat gtccggtggc gccatcgtga cgatcgatct gccgccgttg
     3901 ggcgtgcgct cgctgcgggc ctgggtttgt ctcgatcggg ccgccggaaa tggcggctgc
     3961 atccgcccgc gaattctggc gaacgatgcc aaggggcagc ccttgtggga ggggccggtc
     4021 ctggtgggaa gcgaggcggt tgccgacacg ggaacgatcg gtatagcggg acccgcggca
     4081 gggcagaagt cgatcgtctt gattgtcgat ccggtcgcga ccggccgccc agccggcgcc
     4141 gatccgctcg atatccgcga ccacgccaat tggtgcgacg cgctgctcga gctcgacccg
     4201 gcgatcgtgc aggccgagat cgaccagcgc ctg
//
LOCUS       DASUGB010001055         5789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1647642, whole genome shotgun sequence.
ACCESSION   DASUGB010001055 DASUGB010000000
VERSION     DASUGB010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647642"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFV87_19975"
     CDS             <1..522
                     /locus_tag="VFV87_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5106113.1"
                     /translation="EMHEDAKRLLASLEGGDPTAGEATESAATAADPRTAEKIASSQE
                     GAHKTVMIIESKIEMQDVLRDRLKKHGYCVLIFGDPKRAISRFETDDRAPADCVLFCA
                     QDLGEAALEAFNKFGSMDVSRNIPAILFVDNKQSDLIKAANLGTHRVLLSTPLRVREL
                     RQTLLQLLRREAS"
     gene            complement(571..1692)
                     /locus_tag="VFV87_19980"
     CDS             complement(571..1692)
                     /locus_tag="VFV87_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106114.1"
                     /translation="MWRAKALLLVLLLVPSLASAADESLHRYLYVVCPGIRNYLEFGG
                     AGILVFDIDDGHKFVRRIYTPASREAMPDNIKGVCACAETGRLYFTTRSKLYCLDLAS
                     EKPLWEISPPDGTDRMSITPNGRTLYVPSFEKDTWNVIDAARGEQVATVETKSGAHNT
                     VVSRDGRRMYLGGLKSPILFVADTQTHEIVERVGPLAAAIRPFTVNGASTRAYVCVNE
                     LLGFEIADLTTAKKLFRVEVEGYKTGPVKRHGCPSHGIGLTPDEREVWVVDAANQKLH
                     IFDNTHEPPQQRESLSVREQPGWITFSLDGKFAYPSTGEVIDTATRKTVALLADEEGR
                     EVHSEKMVEIHFRGGKPVANGDQFGVGRVTRAKQTSASP"
     gene            complement(1855..2664)
                     /locus_tag="VFV87_19985"
     CDS             complement(1855..2664)
                     /locus_tag="VFV87_19985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5106115.1"
                     /translation="MPRVAWLTDIHLNFVRERAAVQQFLGEVASGEPDLVLIGGDIAP
                     ARDLLKYLKFIDQVLRRPIYFVLGNHDYYFGSIEKVRKAVKQLCQERPNLHYLSSERE
                     AVQLGHRVGLVGHDGWADGRAGDFDASDVMLNDYLVITDLTNLTKEERKAQLEKRGDE
                     AVKHIRGVLPSALRRFEHVLLLTHVPPYPEACFHKGTLLNGKWLPHYCCLALGEAIVE
                     AARRRPKHRVTVLAGHLHAEGQYNPEPNVSVLVGGAEYGNPAVARVFHLPD"
     gene            complement(2994..3227)
                     /locus_tag="VFV87_19990"
     CDS             complement(2994..3227)
                     /locus_tag="VFV87_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106116.1"
                     /translation="MFRFTIRELILLTLCVALGVEWSFDHSRMARESRAWQSAATILY
                     ADLQAATGESPKWEILGAIKGTFYFLASWIRKK"
     gene            complement(3237..3377)
                     /locus_tag="VFV87_19995"
     CDS             complement(3237..3377)
                     /locus_tag="VFV87_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106117.1"
                     /translation="MQYRLRTLLIVPALGPIVVWMIYHAVQLIPIISREGGMPDRSAP
                     FK"
     gene            complement(3396..3653)
                     /locus_tag="VFV87_20000"
     CDS             complement(3396..3653)
                     /locus_tag="VFV87_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106118.1"
                     /translation="MYRFTIRDVLLATVIAGLAVGWWVDHRRQSAARIAAETKARSLE
                     AKEAQWRAVNLWLSAAMDQESTGFGLSDAEASPRARTGDSR"
     gene            complement(3676..3876)
                     /locus_tag="VFV87_20005"
     CDS             complement(3676..3876)
                     /locus_tag="VFV87_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106119.1"
                     /translation="MFRFTIRDVLWLTTLAAILVMWWMDHRRLDQAGDEARAAAKVSE
                     ERVHMALKEINFLEKIMPIIDR"
     gene            complement(3886..4068)
                     /locus_tag="VFV87_20010"
     CDS             complement(3886..4068)
                     /locus_tag="VFV87_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106120.1"
                     /translation="MRYRLRTLLIVLAIAPPMLAVTWWTAVVIGKRLSPHRASLPKTS
                     DAPGVHRGQLLPGDIE"
     gene            complement(4089..4304)
                     /locus_tag="VFV87_20015"
     CDS             complement(4089..4304)
                     /locus_tag="VFV87_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106121.1"
                     /translation="MFRFTIRDVLWLTVVVGLGVAWLTDHRIQAGERSTGAAKVKMLE
                     RYLGQEGYKMEWSEGNAVLTILRPGSH"
     gene            complement(4314..4562)
                     /locus_tag="VFV87_20020"
     CDS             complement(4314..4562)
                     /locus_tag="VFV87_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106122.1"
                     /translation="MPSRLSSTLLVLLLVTLNVRWDEELARRRRIRFPAGVERAMLRE
                     GRLPRLYELDRQPSSGRIVPDANFPQRLIRPPSGDCQP"
     gene            complement(4572..5039)
                     /locus_tag="VFV87_20025"
     CDS             complement(4572..5039)
                     /locus_tag="VFV87_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106123.1"
                     /translation="MFRFTIRELLLAAALVAVCCGWAGDRWLHRPQRYPLGLDGYCPV
                     TLVETSRWKAGNPRLAAVHEGQRYLLAGPEELQLFEQSPERYAPVSGGADLVRLVDEQ
                     KRTAGRREHGLFTPDGIYLFDSEETLERFSANPKKYAASAVSSSPPSQPPVLK"
     gene            complement(5051..5545)
                     /locus_tag="VFV87_20030"
     CDS             complement(5051..5545)
                     /locus_tag="VFV87_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106124.1"
                     /translation="MRYRLRTLLIVLAILLFAGGAYTLYAFFWRTTIVVPLPYEQAEA
                     RLFSALGMSREALVTTGVAQSDVKGELQKSLRMGIHTHHLLQHVPERRVHFWGDHAYN
                     IWGSGRERIEFDLASAGENRSTITVDYFEYSWFLGFLPTSCQSGRQQERHIVATIFGD
                     KLRQ"
     gene            complement(5551..>5789)
                     /locus_tag="VFV87_20035"
     CDS             complement(5551..>5789)
                     /locus_tag="VFV87_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106125.1"
                     /translation="MARLQLSDSSGDAPGFVTAYLESMESADSICIEFCEPARRGDSV
                     VRFVLTRAEAAGLIGRISTLLARGDGEPDSPSNT"
BASE COUNT         1084 a         1888 c         1724 g         1093 t
ORIGIN      
        1 gagatgcacg aggacgccaa gcggctgttg gcgtcgctcg aaggaggcga tccgaccgca
       61 ggcgaggcaa ccgaatctgc tgcgaccgcc gccgaccctc gcaccgccga gaaaatcgct
      121 tcctcgcagg aaggcgccca caagacggtc atgatcatcg agtcgaaaat cgaaatgcag
      181 gatgtgctcc gcgaccggct gaagaagcac ggttattgcg tgctcatctt cggcgatccc
      241 aagcgggcca tcagccgctt cgaaacggat gaccgggccc cggccgactg cgtgctgttc
      301 tgcgcccagg atctgggcga ggccgcgctg gaggcgttca acaagttcgg ctcgatggat
      361 gtctcgcgca acatccccgc gattctgttt gtcgataaca agcaatcgga cctgatcaaa
      421 gccgccaacc tgggcacgca tcgcgtgctg ctttcgacgc ccctccgggt ccgcgaattg
      481 cggcaaacgc tgttgcagct tctgcggcgc gaagcctcct aatgtgaact gctcgccagt
      541 cgatacccgt ccgtctggcg cacgacgctt ctacggactg gccgaagttt gcttcgcccg
      601 cgtgacgcgg cccacgccga attgatctcc gttggccacc ggcttgccgc cgcgaaagtg
      661 gatttccacc attttctcgc tgtgaacttc ccggccctcc tcatccgcca gcaacgcgac
      721 cgtctttctg gtggccgtgt cgatcacctc gcccgtcgag ggataagcaa acttgccgtc
      781 caggctgaag gtgatccagc cgggttgttc ccgcacggac aggctttccc tttgctgcgg
      841 cggctcgtgc gtgttgtcga agatgtgcag cttctggttg gccgcatcga cgacccacac
      901 ctcgcgctcg tcgggagtca gcccgatccc atgactgggg cagccgtggc gtttgaccgg
      961 gccggtcttg tacccctcga cttccacgcg aaagagcttc ttggcggtcg tcaggtcggc
     1021 gatctcaaac cccagcagct cgttgacgca gacataggcc cgcgtgctcg cgccgttgac
     1081 ggtgaatggg cgaatggccg cggccagcgg gccgacgcgc tcgacaatct cgtgcgtctg
     1141 cgtatcggcg acgaataaaa tcggcgactt gaggcccccc aggtacatcc gccggccatc
     1201 gcggctgacg accgtgttgt gagccccgct cttcgtctcg accgtggcga cttgctcgcc
     1261 gcgtgcggca tcgatgacgt tccacgtgtc cttttcgaac gacggcacgt aaagcgtccg
     1321 gccattgggc gtgatcgaca tccggtcggt gccatccggt gggctgattt cccacagcgg
     1381 cttctcgctg gccagatcca ggcaatacag cttggagcgc gtcgtgaaat agagccggcc
     1441 ggtttccgca caggcacaga cgcctttgat attgtcgggc attgcctcgc gactggccgg
     1501 cgtgtagatt cgccggacga acttgtgtcc gtcgtcgatg tcgaagacga ggattcccgc
     1561 cccgccgaac tccaggtagt tgcgaatccc cggacaaacc acgtacaggt agcggtgcag
     1621 cgattcgtcc gccgcgctgg cgagcgaagg aaccaacaac agcacgagca gcagagcttt
     1681 agcacgccac atgagttcgt ttcctggagg gaagcacagg ccggggcgat atgggcgaca
     1741 ttatatcccg cccgtccacg ggtgctctat taacagcaac tcgacgcgta agcgaggggc
     1801 tcccttgctt acgcgtcggg ttaccgtatc tatcacttca ggttggtcca agcgctagtc
     1861 cggcagatgg aacacccggg ccaccgccgg attgccgtat tcggccccgc ccacgagcac
     1921 cgagacgttc ggctccgggt tgtattgccc ctcggcgtgc agatgcccgg ccagcacggt
     1981 gacgcggtgc ttgggccgcc gcctggccgc ttcgacaatc gcctcgccca gcgccaggca
     2041 gcaatagtgc ggcagccatt tgccgttgag cagcgtcccc ttgtgaaagc aggcctccgg
     2101 atacggcggc acgtgcgtga gcaacagcac gtgctcgaac cgccgtaggg cgctcggcag
     2161 gacgccgcgg atgtgtttga ccgcttcgtc gccccgtttc tccaactgag ccttgcgctc
     2221 ttccttcgtg aggttcgtca ggtcggtgat cacgagatag tcgttgagca tcacgtccga
     2281 ggcgtcgaaa tcgccggccc gcccgtccgc ccagccgtcg tgccccacca gccccacgcg
     2341 atgcccaagc tgcaccgcct cccgctcgct cgaaaggtaa tgcaggttgg gccgctcctg
     2401 gcatagctgc ttcaccgcct tgcggacttt ctcgatgctg ccgaagtaat agtcatgatt
     2461 gcccagcacg aaatagatcg gccgccgcag cacctgatcg atgaatttca ggtacttgag
     2521 cagatcgcgg gccggggcga tgtcgccgcc gatcaggacc aggtccggct cgccgctggc
     2581 cacttcgccc agaaactgct gcaccgcggc ccgctcgcgg acgaagttca ggtgaatatc
     2641 ggtaagccag gcgacacggg gcatcagcgg gaggcaccgg caggcagaag gcgggggacg
     2701 aggcaaacag aggcatcggt agtctcattt cggcatgtaa agattgcgtt cagattccat
     2761 gaagccgccg tcctcacgcc tgccattggg actggtatcc cgatgcgtaa gcgagggacc
     2821 aggccctagc gcaactttcc agcctttttc gcgcctcgcc tcttccgcgc agcatcctct
     2881 taactcccat ccctgaaacc acttgcgccg cgcaataccc ccgtctgagg atgttgcgct
     2941 gttgcgctgt tctggcgaaa cgtcccggga cgtcgcggga aaaaggggac attctacttt
     3001 tttcttatcc agcttgccaa aaagtagaat gtccccttta tcgcacccag gatctcccac
     3061 ttgggcgact cccctgttgc tgcttgcagg tcggcataga gaatcgtcgc tgccgattgc
     3121 catgcacgac tctcccgggc catccgcgag tggtcgaagc tccactccac ccccagcgcc
     3181 acgcataacg tcagcaagat cagctcgcga atcgtgaatc ggaacatggg cggactctat
     3241 ttgaatggtg cgctgcgatc tggcatcccg ccttcccgcg aaatgatcgg aatgagctgt
     3301 accgcatggt aaatcatcca caccacgatc ggccccaagg ccggcacaat cagcagcgtg
     3361 cggaggcgat attgcattga tgaactactc ggcgtctagc gagagtcccc ggttcgtgcg
     3421 cggggcgatg cctcggcgtc ggagagcccg aatcctgtgc tttcctgatc catcgcggcc
     3481 gacagccaca gattgaccgc gcgccactgc gcttccttgg cttcgagcga ccttgccttg
     3541 gtctctgccg caatccgcgc cgcgctttgg cggcgatgat cgacccacca cccgacggcg
     3601 agtccggcaa tgaccgtcgc cagcagcacg tcgcggattg tgaagcggta catggatccc
     3661 caccctttcc gtggtctagc ggtcgattat aggcatgatt ttctcgagaa aattgatctc
     3721 cttcagcgcc atatggacgc gttcctcgga gactttcgct gccgctcgcg cctcgtcgcc
     3781 tgcctgatcc aagcgccggt gatccatcca ccacatcacg agtattgccg caagagtcgt
     3841 cagccacagc acatcgcgga ttgtgaaacg gaacatggag ggctcctatt cgatatcgcc
     3901 gggaagcaac tgcccccgat gcacgccggg ggcgtcgctc gtttttggca atgaggcgcg
     3961 atgtggcgac aagcgcttcc cgatgaccac tgccgtccac caagtcaccg ccaacattgg
     4021 cggcgcgatg gccagcacaa tcagcagcgt gcggaggcga tagcgcatcg aggcgttggg
     4081 agctgcggct aatggctgcc tggccggaga atcgtcaata cagcgttgcc ctcactccat
     4141 tccattttat acccctcctg gcccaaatag cgctccagca ttttcacctt ggccgcaccc
     4201 gtgctccgtt cgcccgcctg aatccggtgg tcggtgagcc acgccacgcc cagccccacc
     4261 accacggtca accacagcac gtcgcggatt gtgaaacgga acatgcgagg ctcctacggc
     4321 tggcagtcgc cggatggcgg gcggatcagc cgctgcggga aattggcgtc gggcacgatg
     4381 cggcccgacg agggctggcg gtcgagctcg taaagacgcg gcagccgccc ttcgcggagc
     4441 atcgcccgct cgacacccgc gggaaagcgg attcgccgtc gccgggccag ctcctcgtcc
     4501 cagcgcacgt tcagcgtcac cagcagaagc accagcagcg tcgaactaag gcgggaaggc
     4561 atcggcagac cctatttgag aactggcggc tggctgggcg gcgagctgct gacggcgctg
     4621 gccgcgtatt tcttgggatt ggcggagaat cgctcgagcg tttcttcgct gtcgaataaa
     4681 taaatgccat ccggcgtgaa taggccgtgc tcacgccggc cggccgtccg tttttgctca
     4741 tcgaccaggc ggaccaagtc ggcgcccccg ctgacgggag cgtaacgctc cggagactgc
     4801 tcgaaaagct gcaattcctc cgggccggcc agcaagtacc gctgcccctc gtggaccgcc
     4861 gccagccgcg ggttgccggc tttccagcgc gacgtttcaa ccagcgtgac agggcaatat
     4921 ccatccagcc cgagcggata ccgctgcggg cgatgcagcc agcgatcgcc cgcccatccg
     4981 cagcagaccg ccaccagcgc cgcggccaga agcaactcgc gaatggtaaa gcggaacatg
     5041 ccagcgaccc ttattgtcgg agtttgtcgc cgaatatcgt tgcgacgatg tgccgctctt
     5101 gttggcgccc ggactggcaa ctggttggca gaaatccgag gaaccacgag tattcgaagt
     5161 agtcaaccgt gatcgtactc cggttctccc cggcagacgc aagatcaaat tcaatccgct
     5221 cccggccgct tccccaaatg ttgtacgcgt gatcgcccca aaaatgaacg cgccgctctg
     5281 gaacgtgctg aagcaagtga tgcgtgtgga ttcccattcg cagcgacttt tgaagttctc
     5341 ctttgacgtc tgattgcgca actccggttg tgacaagtgc ctctctgctc atgccgagag
     5401 ccgagaatag ccgggcctcc gcttgctcgt acggcaacgg cacgacgatt gtggttcgcc
     5461 agaaaaatgc gtagagcgtg tacgccccgc cagcgaacaa gagaatcgcc agcacaatca
     5521 gcagcgtgcg gaggcggtaa cgcatagcgt tcaggtattg ctgggtgaat caggctcgcc
     5581 gtcgccccgc gccaacagcg tagagattcg cccgattagc ccagcagctt cggcgcgcgt
     5641 gaggacgaac cggaccacgc tgtcgccgcg tcgggcgggt tcgcagaact cgatgcagat
     5701 gctgtcggcg ctttccatgc tctccagata ggccgtcaca aagcccggag catcaccgct
     5761 tgagtcgcta agttgcaggc gcgccatgc
//
LOCUS       DASUGB010001056         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2063265, whole genome shotgun sequence.
ACCESSION   DASUGB010001056 DASUGB010000000
VERSION     DASUGB010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063265"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFV87_20040"
     CDS             <1..918
                     /locus_tag="VFV87_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5106126.1"
                     /translation="YGGKHTNDAPIEAARLAKAAGRPVKLVWSREEEFTWAYHRPAGV
                     IDVNAGVDKDGKLTAWDFVNYNSGGSSIRPLYDIPNLRTASRGSRSPLKQGSYRALAA
                     TANSFARECHIDDLAHAAGIEPLHFRLQNTSDERLRAVLIAATDKFGWTTRQPAAGHG
                     FGIAGSSEKGSYVATCAEVAIDRERGKVQVLRAVTAFECGAIVNPRHLENQVEGAVMM
                     GLGGALFEAIEFDSGKITNASFGGYRVPRFADLPELETVLVNRLDLPSAGAGETPIIA
                     IAPAIGNAIFHATGQRLRSLPMAPRGLTT"
     gene            complement(1162..2151)
                     /locus_tag="VFV87_20045"
     CDS             complement(1162..2151)
                     /locus_tag="VFV87_20045"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX5106127.1"
                     /translation="MPRILIKSVSIVALCAGAVAGCASVPRRAALPADGSAPQPRTLA
                     QALQLDPIVPPERHTPASESRGKPQLDSAVAVANDKPSNDRQWIPEHRLLASAEFDGD
                     RATIHNIRNAEFFSYRDCLVDYYDKSYDLSKLRTVDFIMIPFNDARYLAHTMLTFGFE
                     NGDYLGVSVEVRLEQGESYDAAIGLFNQFELIYVVADERDLIRVRTEYRDVDVLIYRT
                     KATPEQARRLFVDIMRRVNKLKDEPEFYDTLSNNCTTNIARHINTLAPGSVPHDFRVL
                     LPGYADNLAYELGLIDNSLPFAEVRRRARVNDLALRYKGDPHFSQRIRGEQVR"
     gene            complement(2296..>2490)
                     /locus_tag="VFV87_20050"
     CDS             complement(2296..>2490)
                     /locus_tag="VFV87_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5106128.1"
                     /translation="LEFQEEPVEQLLARHYFEPKRPLRYCHPRDLLLQVKAFCEFHDQ
                     PLVLTERALEVAVKNYFAGL"
BASE COUNT          464 a          756 c          842 g          428 t
ORIGIN      
        1 tacggcggca agcacaccaa cgacgctccg atcgaggcgg cccgcctggc gaaggcggca
       61 ggccggcccg tcaagctcgt ttggtcacgc gaggaagagt tcacctgggc gtaccatcgc
      121 ccggccggcg tcatcgacgt gaacgccggt gtggacaagg acggcaagct cacggcctgg
      181 gattttgtga attacaattc gggcggctcg tcgatccggc cgttatacga cattcccaac
      241 ctgcggacgg cgtcgcgtgg ctcgcgctcg ccgttaaaac agggttctta ccgggccctg
      301 gctgccacgg ccaacagctt tgcccgcgaa tgccacatcg acgacctggc ccacgccgcg
      361 ggcatcgaac cgctgcactt tcgcctgcag aacaccagcg acgagcggct gcgggccgtg
      421 ttgatcgccg cgacagacaa gttcggttgg acaacgcggc agccggccgc gggccacggg
      481 tttggtattg cgggcagctc cgagaaaggg agctacgtgg caacctgtgc cgaagtggcc
      541 atcgatcgcg agcgcggcaa ggtgcaggtg ctgcgggcgg ttacggcttt cgagtgcggt
      601 gcgatcgtga atccccggca cctggaaaac caggtcgagg gggcggtgat gatgggcctg
      661 ggcggcgcgc tcttcgaagc gatcgaattc gacagcggca agatcaccaa cgccagcttc
      721 ggcggctacc gcgtgccgcg attcgctgat ctgccggaac tcgaaacggt gctcgtgaac
      781 cggctggacc tcccctcggc cggagctggc gaaacgccga tcatcgccat cgccccggcg
      841 atcggcaatg cgatcttcca cgccaccggc cagcgcctcc gctcgctgcc gatggcgccc
      901 cgcggcctga cgacgtgagg ggctcgcgct cttgggagga cgttggcgtc acagaaagaa
      961 gcttttccca atccttgcac aggctggcaa taagcagagt tatgatcatc catatcgtat
     1021 ggaatgttga cgccgctggt gtgctacacg gaatggcgtg actggaaatc gggcgaaacg
     1081 atcgctcgcg agcgcgaggc ggccggcctg ctgcaggaac agctcccgcg tcaaaatcac
     1141 ccggattgag cccctgagag ttcagcgaac ctgctcgccg cgaatccgct gcgagaagtg
     1201 cggatcgccc ttgtagcgga gggccaggtc gttcacgcgc gcccgcctac ggacttccgc
     1261 gaagggcagc gaattgtcga tcagccccag ctcgtaggcc aggttgtcgg catagccagg
     1321 caggagcacg cggaaatcgt ggggcacgct gccgggagcg agcgtgttga tgtgccgcgc
     1381 gatgttggtg gtgcaattgt tgctgagcgt gtcgtagaac tcgggctcgt ccttgagctt
     1441 gttgacgcgc cgcatgatat cgacgaacag ccgccgggcc tgctcgggcg tggccttggt
     1501 gcggtagatc agaacatcga cgtcgcggta ctcggtgcgc acgcggatca gatctcgctc
     1561 gtcggcgacg acgtagatca gctcgaactg gttgaacagg ccgatcgccg cgtcgtagct
     1621 ttcgccttgc tccaggcgga cctcgaccga aacgcccagg taatcgccgt tctcgaagcc
     1681 gaaggtgagc attgtatggg cgagataccg cgcgtcgttg aatgggatca tgatgaaatc
     1741 caccgtccgc agcttcgata agtcatacga cttgtcgtag taatcgacga ggcaatcgcg
     1801 gtacgagaag aactcggcgt tgcggatgtt gtggatcgtg gcgcggtcgc catcgaattc
     1861 ggcagacgcg agcagccgat gctcgggaat ccactggcgg tcgttgctgg gcttgtcgtt
     1921 ggcgacggcg accgctgagt cgagctgcgg ctttccccgt gattcgctgg cgggcgtatg
     1981 gcgctccggc ggcacgattg gatcaagctg cagggcctgg gccagcgtcc gcggctgcgg
     2041 agccgagccg tcggcgggga gcgcagcccg acgcgggacg ctcgcgcagc cggctaccgc
     2101 gccggcgcag agcgcgacaa tcgagacgga cttgatgaga atccgcggca tgcttcccgc
     2161 tgtgaaacgt gcgctacgat ccgacaagcg gtcggatcaa tagcaggcag gtcgcgccca
     2221 ggcaagggcg aagggtgcag gacactgcta gcaacgcctt gactgccgac ggctgacaac
     2281 tgaccacgaa caactttaca gccccgcgaa gtaattcttc acggcaactt ccagcgcccg
     2341 ctcggtcaac acgagcggct ggtcgtggaa ttcgcagaac gccttgacct gcaagaggag
     2401 gtcgcgggga tggcaatatc gcagtggccg cttgggctcg aagtagtgcc gggccaggag
     2461 ctgctcgacg ggctcctcct ggaattccag
//
LOCUS       DASUGB010001057         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2063299, whole genome shotgun sequence.
ACCESSION   DASUGB010001057 DASUGB010000000
VERSION     DASUGB010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063299"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VFV87_20055"
     CDS             <1..173
                     /locus_tag="VFV87_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5106129.1"
                     /translation="SKGTFGHGGAFGTQAWIDPEQGFFVILLIQRTGLPNSDASDLRK
                     ELQRLAVEAIRK"
     gene            415..2325
                     /locus_tag="VFV87_20060"
     CDS             415..2325
                     /locus_tag="VFV87_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5106130.1"
                     /translation="MPLLAAAADFTWQGGFTLALIVAVFLALAFTTLAPDIILIGGVI
                     LLVVSGVLSPGDALAGLSNEGMVTVGVLYIVGAGVRETGGVDWIAKSLFGRPKSLLTA
                     IIRLVFPTLGLSALMNNTPLVAMLLPAVDDFAKTQRISPSKLMIPLSYAAILGGTMTL
                     IGTSTNLVVQGQVQKEIDKQAAAAKQGAAVPPGQLVQPLGMFDITWVGVPAALIGGIY
                     VVFAARWLLPDRKAPLSTVADPREYTVEMQVEADSPLAGKSIEAAGLRHLPGLYLAEI
                     DRDGSVIPAVSPREVLRAGDRLLFVGVVESVVELQKIRGLIPATDDVFQLKAPRPQRV
                     LIEAVVSNSCPLVGMTIRDSRFRSHYNAVVVAVARNGQRLHQKIGDIVLHAGDVLLVE
                     AHPSFAEQHKNSRDFFLVSQIDGSTPPKHELALVALGILALMVLAISAKDLMAVAAAA
                     LPFLQPAADWLPRITPIMASLVAAAAMIIFRCASLETARKSIDWQVLLAIAAAFALGA
                     ALDKSGAAEVIAQRTIALAGGNPWITLALIYLVTVVVTELITNNAAALLMFPFAFNAA
                     GQLGVNYMPFVIVVMMAASAGFATPIGYQTNLMVYGPGGYRFSDYVKVGVPLDILIGV
                     VTVAIAPLVYPF"
BASE COUNT          400 a          778 c          746 g          450 t
ORIGIN      
        1 tgagcaaagg aacgtttggc cacggcgggg cattcgggac gcaggcctgg atcgacccgg
       61 agcagggctt cttcgtaatc ctgctcattc agcggaccgg actaccgaat tcggacgcct
      121 ccgatctgcg gaaggagctg cagcggctgg ccgtcgaggc gattcgtaag tagctcatat
      181 acaacacttt acgacgattt accagcaaat cctccgattg gacttcccac cgatcgcatg
      241 atacattact ttgtagatta acttaataat gaatagacag ccgcgttgcc gaatagtaac
      301 ccgaagcgta agcgagggag cttcctgtga aggcgtaagc gaaggactcc ctcgcttacg
      361 cttcgggtta ctgttacgca ggccgattcg gcacaccaac acctatctcc tgccatgccc
      421 ctcctcgccg ctgctgccga cttcacctgg caaggcgggt tcacgctcgc gctgatcgtg
      481 gcggtgttcc tcgcgctggc gttcacgacg ctcgcgccgg atatcatcct gatcggcggc
      541 gtgatcctgc tggtcgtcag cggcgtgctt tcgccgggcg acgcgctggc gggcctctcg
      601 aacgaaggga tggtcacggt cggcgtgctg tacatcgtcg gcgccggcgt ccgcgaaacg
      661 ggcggcgtcg actggatcgc caagagcctc ttcggccggc ccaagtcgct gctgaccgcg
      721 atcatccgcc tcgtctttcc cacgctcggc ttaagcgccc tgatgaacaa cacgccgctg
      781 gtggcgatgc tgctgccggc ggttgacgac ttcgccaaga cgcagcgaat ctcgccctcc
      841 aagctgatga tcccgcttag ctatgccgcg atcctcggcg gcacgatgac gctgatcggc
      901 acgagcacga atcttgtggt ccaggggcaa gtgcagaaag agatcgacaa gcaagcggcc
      961 gcggccaagc aaggtgcggc cgtcccccca ggccagctcg tccagccgct cggcatgttc
     1021 gacatcacct gggtcggcgt gccggcggcg ctcatcggcg gaatttacgt tgtcttcgcg
     1081 gcccgctggc tgctccccga tcgaaaggcc ccgctgagca ccgtcgccga cccgagggaa
     1141 tatacggtcg aaatgcaggt cgaagccgac agcccgctgg ccggcaaatc gatcgaagcg
     1201 gccgggctgc ggcacctgcc ggggctgtat ctggccgaga tcgaccgcga cggcagcgtg
     1261 attcccgcgg tttccccccg cgaagtgctg cgggccggcg accggctgct gttcgtgggg
     1321 gtggtcgagt cggtcgtcga attgcagaag attcgcggcc tgattcccgc gaccgacgac
     1381 gtctttcagc tcaaggctcc ccggccgcag cgggtgctga tcgaggcggt cgtcagcaat
     1441 tcctgcccac tcgtgggaat gacgatccgc gactcgcgct tccgcagcca ctacaacgcg
     1501 gtcgtcgtgg cggtcgcccg caacggccag cggctgcacc agaagatcgg cgacatcgtg
     1561 ctgcacgccg gcgacgtgct gctggtcgag gcccatccct ccttcgccga gcagcacaag
     1621 aacagccgcg atttcttcct tgtcagccag atcgacggct cgacgccgcc caagcacgag
     1681 ctggcgctgg tcgcgcttgg cattctggcc ctgatggtcc tcgcgatctc ggccaaggac
     1741 ctgatggcgg tcgccgccgc cgcgctcccg ttcctgcagc cggcggctga ctggctgccg
     1801 cggatcacgc cgatcatggc ttcgctggtg gcggccgcgg cgatgatcat cttccgctgc
     1861 gcttcgctcg aaacggcccg caagagtatc gactggcagg tgctgctcgc catcgcggcg
     1921 gcattcgccc tgggagcggc gctcgacaaa tcgggggccg ccgaagtcat tgcccaaaga
     1981 acgatcgcgc tggccggcgg caatccgtgg attacgctgg cgctgatcta cctggtgacg
     2041 gtcgtcgtca ccgagctgat caccaacaat gccgccgcgc tattgatgtt cccgtttgcc
     2101 ttcaatgccg ccgggcaatt gggcgtgaac tacatgccct ttgtgatcgt cgtgatgatg
     2161 gccgccagcg ccggctttgc cacgccgatc gggtaccaga cgaacctgat ggtttatggc
     2221 cccggcggct accgattcag cgattacgtc aaggtcggcg tgccgctgga tatcttgatc
     2281 ggcgtcgtca cggttgccat cgctccgctg gtgtatccgt tctgatgccg ccagtcggac
     2341 ggggtcggga gtcaattgcg gccgacgacc cacc
//
LOCUS       DASUGB010001058         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_952233, whole genome shotgun sequence.
ACCESSION   DASUGB010001058 DASUGB010000000
VERSION     DASUGB010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952233"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..891
                     /locus_tag="VFV87_20065"
     CDS             343..891
                     /locus_tag="VFV87_20065"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5106131.1"
                     /translation="MANQTKPDTADNASVLTSGARSEFQIKSEAFDLGKRIPPRHSGE
                     GEDRSPPLVWGGVQEGTQELALIIADPDAPGGTWYHWVLYNIPADVTALAEGLPREAK
                     LTEPKGARQGLNSWPSENVGYRGPMPPPGHGPHRYYFILYALDTHIDLDPAQATAQNL
                     LAVIKGHVLAEARTMGTYERKR"
     gene            complement(943..>2074)
                     /locus_tag="VFV87_20070"
     CDS             complement(943..>2074)
                     /locus_tag="VFV87_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106132.1"
                     /translation="TAEPAEAALSESAPAAEASADAPHPSGVPGTLPDSDGVVEVVKE
                     RFPNGAVKVEREMTQDLQGNYVLQGAWRHFDEQGRLIIDGRHDHGQKSGLWRRFYRGT
                     EAPLYSSIPYKDFSAPYISQATFHGGQMHGRWTITDSKQRKVHEIEFCDGERHGKATW
                     YYPNGAIMLQAQYEHGRVNGDVMKFAPDSSLIAQENYQAGRKLAPKTEYYDTEQTSKK
                     LETTYLHGVLVVKTPDNWETSTLATFETRGQDERHGPFTAWHGNGQPAKQGEYRYNLP
                     VGKITFWYENGQKQMAGMYVDGRQEGVWTWWHANGQKSITGEYHDAQPVGKWSWWQAS
                     GKIAQRADLSQRPLSAPTVMPDAESEPREANLKLLEPGLPLR"
BASE COUNT          362 a          646 c          666 g          400 t
ORIGIN      
        1 ggaagggctg ccgcggcggg gacgatcgca atgaagtacc agccctcagc cgtcgccacg
       61 cccagtccca tggcgcacac cgcggccagg acgagcaatc cggcgacgac caacccgagc
      121 ctggcggcgt caaacattcc actcggttcg tattgcgttc ccatcgtgcc tgccccagtg
      181 attggatttc ggccagtgaa agtctaggcc ggcggtgtcc gcgcggcgcg gctctgccta
      241 aaatgccgaa gattccagtc atgagggcga ttgggcgaac tcgtggtcca caagttgccg
      301 ttgattaccc gcggccttga tccgcgagcg ggaggtagtg aaatggcgaa tcaaacgaag
      361 ccggatacgg cggacaatgc cagcgtcctc acgagcgggg cgcggtcgga gtttcagatc
      421 aagagcgagg cgtttgacct tggcaagaga attcctccga ggcactcggg cgagggtgag
      481 gaccgctcac cgccgctggt gtggggcggc gtgcaggagg ggacgcagga attggctttg
      541 atcatcgccg atcccgatgc gcccggaggg acctggtatc actgggtcct ctacaacatc
      601 ccggcagacg tgacggctct cgccgaaggg ctgccacgcg aggcgaagct gacggagccg
      661 aaaggagccc gccaggggct gaattcatgg cccagcgaga acgtcggcta tcgcgggccg
      721 atgccgccgc cggggcacgg tccgcaccgc tattacttca tcctctacgc gctggatacg
      781 cacatcgatc tcgaccccgc ccaggcaact gcccaaaatc tgctggccgt gatcaaaggc
      841 cacgttctcg ccgaagcccg gacaatgggc acctacgagc ggaaacgatg agccgtagga
      901 tgggtatccc cgtccggcgg tgagccgtcg cgccgaccaa cgctaccgca gcggcaggcc
      961 gggctcgaga agcttcaggt tggcctcgcg cggttcgctc tcggcatcgg gcatgaccgt
     1021 aggagcggaa agtggccgct ggctcaggtc ggctcgctgg gcaatcttgc cgctggcctg
     1081 ccaccaggac catttgccga ccggctgggc gtcgtggtat tcgccggtga tcgacttctg
     1141 gccgttggca tgccaccagg tccagacgcc ttcctgccgg ccatcgacgt acatccctgc
     1201 catctgcttc tggccgttct cgtaccagaa ggtgatcttg ccgacgggaa ggttatagcg
     1261 atactcgccc tgtttggcgg gctggccatt gccgtgccac gcggtgaagg gtccgtgccg
     1321 ttcgtcctgg ccgcgggtct caaacgtggc gagcgtactg gtctcccagt tgtcaggcgt
     1381 cttgaccacc aacacgccat gcaggtacgt cgtctcaagt ttcttgctcg tctgctcggt
     1441 gtcgtaatac tcggtcttcg gagcgagttt tcggcccgct tggtagtttt cctgggcgat
     1501 gagcgaggaa tcgggggcga acttcatcac atcgccatta acgcgaccat gctcgtattg
     1561 ggcctggagc atgatcgctc cgttgggata gtaccaggtg gctttgccgt gccgctcgcc
     1621 gtcgcaaaac tcgatttcgt ggactttgcg ctgcttggaa tcagtaatcg tccacctgcc
     1681 gtgcatctga ccgccgtgga atgtggcttg cgagatatac ggggcggaga aatccttata
     1741 cggaatcgac gagtagagcg gggcctccgt cccgcgatag aaccgccgcc acaggccgga
     1801 tttctgccca tgatcgtggc ggccgtcgat gatcaggcgc ccctgctcgt cgaaatgccg
     1861 ccacgcgcct tgcaggacat agttgccctg gaggtcctgc gtcatttcgc gctcaacctt
     1921 gaccgcgcca ttggggaatc tctctttgac gacttccacg actccatcgg agtcaggcag
     1981 cgttcccggc acgccgctcg ggtgaggagc atccgcggac gcttcggcag ctggcgcact
     2041 ttcactgagc gctgcctcgg ccggttcggc ggtg
//
LOCUS       DASUGB010001059         5230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1922963, whole genome shotgun sequence.
ACCESSION   DASUGB010001059 DASUGB010000000
VERSION     DASUGB010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922963"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..886
                     /locus_tag="VFV87_20075"
     CDS             119..886
                     /locus_tag="VFV87_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5106133.1"
                     /translation="MPCPTCGSPITVAGPRPAGTPFATHPAPPPNPHGTASQQFRRNP
                     FWYVFHGVEMPQYQAGCTPRATRRTQKRMLAGLGVVVLGLALLTFAIAAARFGLWFGL
                     GFMFFGLGVAFVPLLFGFYFVAAGLLESDLLMMSRKGRGVRYLFGDQGARWFFIIAGC
                     PMLLIGLMIQLGFVAGAAIGRDPNRNRNAPVQAADDRPQHVPLDAAQAAANDRARKQM
                     IEDFRRENADLLQNPEVAAEFEKKLREQFPDRKAPAP"
     gene            complement(913..2229)
                     /locus_tag="VFV87_20080"
     CDS             complement(913..2229)
                     /locus_tag="VFV87_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106134.1"
                     /translation="MRARSRIAVTLVVIVASLFLSHWAFGQEYEEEPIRYSDSEPNNR
                     VSQVLERIEAGELKLKFSDRLGYLPALLQELNVPASSQMLVFSKTSLQRQRIAPRTPR
                     AIYFSDDVYIGYCQQGAVLEISAVDPQLGAVFYTLDQDQYETPLVKRQTDNCLLCHGS
                     GHTKDVPGHVVRSVFSDAGGLPILSSGSYRINQASPLAQRWGGWYVTGTHGEQKHLGN
                     LIIPTRDVPERLDNSAGMNVTDLADRFNTGKYLTPHSDIVALMVLEHQAEAHNLLTRA
                     SMHTRQAMHYQQALNRELGEPADKVWDSTKSRIKSVCEPLVEYLLFSEEAQLASPIHG
                     TSGFAEEFVRRGPRDRQGRSLRDFDLSRRLFQYPCSYLVYSPNFQELPREAKDYVLRR
                     MWEVLSGADQSEPFAHLSADDRQAILEILRETLPDLPDSWNSTDGA"
     gene            2372..2824
                     /locus_tag="VFV87_20085"
     CDS             2372..2824
                     /locus_tag="VFV87_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5106135.1"
                     /translation="MFSQTVEYALRAVVHLASEAPEARTTDQIAAATRVPRAYLSKVL
                     QSLVRGGVVTSQRGIGGGMMLARPAAEMTILEVVNAVEPIKRIRTCPLGIATHGVRLC
                     PLHSRVDQALALVEEAFGNSTLAEILAEPSSSVPLCPYPNLKTCVRQE"
     gene            3030..3812
                     /locus_tag="VFV87_20090"
     CDS             3030..3812
                     /locus_tag="VFV87_20090"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5106136.1"
                     /translation="MTPARELPTLRVVTPPADEPQTDEGKLVPTPELLEELEQASDRL
                     ELAEPEDILRWAAARFAPRFTMATAFGPEGMAIIHMLSEIAPETPIFNLDTGYQFQET
                     LELREEVKRRYGIEVELKRADTTVEQYEQIHGGPLYRTNPDQCCFDRKVKVLQQAAIG
                     MHAWASAIRRDQSPDRAKAPIVGWDKKFGLVKVSPLANWTKQKVWGLITKHNIPYNPL
                     HDQGFTSIGCWPCTRAVTIGEDERAGRWSGFAKTECGLHSQG"
     gene            3841..4269
                     /locus_tag="VFV87_20095"
     CDS             3841..4269
                     /locus_tag="VFV87_20095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5106137.1"
                     /translation="MIVSAKTEYACIAVLELAARHASGEPVRIRDIAETHGVPSRFLV
                     QILLQLKSAGLVQSIRGAAGGYQLAREPDEISLLDVMTVVDPQLAQVPASATRSTATT
                     RALQRTWKRVATAERDLLAEVTFADLVEQLKGTTEGMYYI"
     gene            4275..5093
                     /gene="trpC"
                     /locus_tag="VFV87_20100"
     CDS             4275..5093
                     /gene="trpC"
                     /locus_tag="VFV87_20100"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5106138.1"
                     /translation="MSIVTCQLVFVPTILEQIVASKRAEIERAKAAIPIAELRARLPD
                     APAVRDFFAPLAAEGPIKLIAEVKKASPSKGVIRADFDPVAIGRTYEAHGAMCLSILT
                     DEPYFQGSLEYLKQVRQAVAIPVLRKDFILDSYQLTEARAAGADAVLLIAECLDDCHL
                     RKLHNEALELGMTPLVEFYEPANLDRVLAAGATLVGVNNRDLRTFEVDLEHTIRMRQR
                     VPQGCVLVGESGILARADALRLESAGVEAMLVGESLMREADIGAAVDRLLGVSV"
BASE COUNT          952 a         1613 c         1683 g          982 t
ORIGIN      
        1 tctggcgtag gttgtgcagc tcatttttac cgccaatcga taaccgcctt tgcctctcat
       61 cgtcgaatgt cgctgtcgcc agcggtttag tgccgccgat catctgatcg ggtccgtcgt
      121 gccttgcccg acatgtgggt cgccgatcac ggtcgccggt ccgcggccag ccggcactcc
      181 cttcgcaacg catcccgccc cgccgcccaa tccccacggc accgcgtcgc agcagtttcg
      241 ccgcaacccg ttctggtatg tctttcacgg cgtcgagatg ccccagtacc aggcgggatg
      301 cacgccgcga gccacgcgcc gcacgcagaa gcgcatgctc gccggcctgg gcgtggttgt
      361 gctgggcctc gcgctcttga ccttcgccat cgccgcggcg cggtttggtc tctggttcgg
      421 cttggggttc atgttcttcg gcctcggcgt cgccttcgtg ccgctgctgt tcggcttcta
      481 cttcgtcgcc gccggattgc tcgaatcaga cctcctgatg atgtcgcgaa agggccgcgg
      541 cgtccgctac cttttcggcg accagggcgc ccgctggttc ttcatcattg ccggctgtcc
      601 gatgctgctg atcggcctca tgatccagct tggctttgtg gccggcgccg cgatcggccg
      661 cgatcccaac cgcaaccgca acgccccggt ccaggccgcc gacgatcgcc cgcagcacgt
      721 gccgctcgat gccgcccaag ccgctgccaa cgaccgggcc cgcaagcaaa tgatcgagga
      781 cttccgccgc gaaaacgccg atttgctcca gaacccggaa gtcgccgccg agttcgagaa
      841 gaaactccgc gagcagtttc cggaccgcaa ggcgccggct ccgtgagtgt ccggccagga
      901 attggaaccg cgtcaggcgc cgtcggtgga attccaggaa tcgggcaagt cgggcaatgt
      961 ctcgcggagg atttcgagga tggcttgccg gtcgtcggcg gagaggtggg cgaacggctc
     1021 cgactgatcg gcgccgctga ggacctccca cattcgccgc aggacatagt ctttggcttc
     1081 gcggggaagt tcctgaaagt tcggcgagta cacgaggtag ctgcacggat attggaacaa
     1141 tcgccgcgag agatcgaaat cgcgaagcga gcgcccctgg cggtcgcgcg ggccgcggcg
     1201 gacgaactcc tcggcgaagc ccgacgtgcc gtggatggga gatgcgagct gagcttcctc
     1261 gctaaacagc aggtactcga ccagcggctc gcaaaccgat ttgatccgcg atttggtgct
     1321 gtcccaaact ttgtcggcgg gctcgcccaa ctcgcggttg agggcctgct ggtagtgcat
     1381 ggcctgccgc gtgtgcatgc tggctcgcgt gagcaggttg tgggcctcgg cctgatgctc
     1441 gagcaccatg agtgcgacga tgtcgctgtg cggcgtcaga tattttcctg tattgaagcg
     1501 gtcggccagg tcggtcacgt tcatcccggc cgaattatcg agacgctccg gcacgtcgcg
     1561 cgtcgggatg atcaggttgc ccaggtgctt ctgctcgccg tgcgtcccgg tgacatacca
     1621 cccgccccac cgctgggcga gcgggcttgc ctggttgatg cggtacgagc ccgaggagag
     1681 tatcggcaag ccgcccgcgt cggagaagac gctgcggacg acgtgccccg gcacgtcctt
     1741 ggtgtgaccc gagccgtggc agagcaggca attgtcggtc tggcgcttga ccagcggtgt
     1801 ctcatactgg tcttgatcga gcgtgtagaa aaccgcgcca agctgcggat cgaccgccga
     1861 gatctcgagc acggcgccct gctggcagta gccgatatag acgtcgtcac tgaaatagat
     1921 cgcccgcggc gtgcgcggcg cgatccgctg tcgctgcagg ctcgtcttcg agaagacgag
     1981 catctgcgac gatgccggga cattgagttc ctgcaagagc gccggcaaat agccgagccg
     2041 gtcgctaaat ttcagcttta gctcgcccgc ctcgatccgc tcgaggactt gcgaaacgcg
     2101 attgttcggc tcggagtcgc tgtaacgaat cggctcctct tcgtattcct ggccgaatgc
     2161 ccagtggctg agaaacaggc tggcaactat taccaccagc gtaacggcga ttcttgagcg
     2221 ggcgcgcatg gtcggttgcc tccgaggagg aattgtcatt gactttgaaa ctcgtcttga
     2281 tcaaaattgg actcttacat ccagtttcac ataagatatc ggctgctaat gggatgtcaa
     2341 tatccaattt aggcgagaca ggactttttt gatgttttcc cagaccgtcg aatatgctct
     2401 gcgggccgtc gtccacttgg ccagcgaagc tcctgaggcc cgaacgacgg accagattgc
     2461 agccgccacg cgcgtgccgc gggcgtacct ttcaaaagtg ctgcaaagcc ttgtccgggg
     2521 cggagttgtg acgtcccaac gaggcatcgg cggagggatg atgctcgccc ggccggcggc
     2581 ggaaatgacg attctggagg tcgtcaacgc agtggagccg atcaagcgaa tccgtacctg
     2641 cccgctgggc atcgctaccc acggcgttcg gctttgcccg ctgcacagcc gcgtcgatca
     2701 ggccctggcg ctggtggaag aagcgttcgg caattcgacg ctggctgaga tcctggcgga
     2761 gccgtccagc agcgtgccgc tctgcccgta tccgaacctg aagacgtgtg tgcggcagga
     2821 gtgatcgtca catatcccgc gtttgaccgc tacaagctgt agaaccggcc ccacgtgggc
     2881 cgatgaaatc cagtggcgcg gccgaatgtt gacttagatt agcaacatgg ataagattcg
     2941 gctccggagt ggccttggcg gctaagttgc tgccgggccg gaggttggtg cgattcatga
     3001 cccttgccgc tcgccgatgg gcccccgcga tgacgcccgc tcgcgaattg ccgacgctcc
     3061 gggtcgtcac gccccccgcg gatgagccgc agacggacga aggcaagctc gtccccacgc
     3121 cggaactgct cgaagagctg gaacaagcca gcgaccggct ggagctggca gagccggagg
     3181 acatcctccg ctgggcagcg gcccgcttcg cgccaaggtt cacgatggcc acggccttcg
     3241 gtccggaagg gatggcgatc atccacatgc tgtcggaaat cgcccccgaa acgcccatct
     3301 tcaatctcga cacgggttat caattccagg aaacgctgga gttgcgcgag gaagtgaagc
     3361 gccgttacgg catcgaggtg gaattgaagc gggccgacac gacggtcgag cagtacgaac
     3421 aaattcacgg aggaccgctg tatcgcacca atcccgacca gtgctgcttc gaccgcaaag
     3481 tgaaggtcct ccagcaggcg gcgatcggca tgcacgcctg ggccagcgcc atccgccgcg
     3541 accaaagccc cgaccgggcc aaggcgccga ttgtcggctg ggacaagaaa tttggcctgg
     3601 taaaggtcag cccactggcc aactggacca agcaaaaggt ctggggcctg atcacgaagc
     3661 acaacattcc gtacaacccg ttgcacgatc agggcttcac gagcatcggc tgctggccct
     3721 gcacgcgagc cgtcacgatc ggcgaagacg agcgggcggg ccgctggagc ggctttgcca
     3781 aaacggaatg cgggctgcac agtcaaggat gatcgccacg ccctcgatga tcgccaagcc
     3841 atgatcgtct cggctaagac cgaatacgcc tgcattgccg tcctggagct cgccgcccgg
     3901 catgccagcg gcgagccggt gcggattcgc gacattgccg aaacccacgg cgtcccctcg
     3961 cgattccttg tgcagatcct gctgcagctc aaaagtgccg gcctcgtgca gagcatccgc
     4021 ggcgcagccg gcggctatca gctcgcccgc gaaccggacg agatttcgct cctggatgta
     4081 atgacggtgg tcgatccgca gctcgcccag gtgccggcca gtgccacgcg gagcaccgcc
     4141 acgacgcggg ccctgcagcg aacgtggaag cgtgtcgcca ctgccgagcg ggatttgctg
     4201 gccgaggtaa cgtttgccga cctcgttgag cagcttaagg gaacgacgga agggatgtac
     4261 tatatttagg tcgcttgtca atcgtcactt gtcagttggt gtttgtgcct acgattctcg
     4321 agcagatcgt cgccagcaag cgcgccgaga tcgagcgcgc gaaggcagcc attccgatcg
     4381 ccgaactgcg ggccaggctg cctgatgcgc cggccgtccg cgatttcttt gcgccgctgg
     4441 ctgcggaggg gccgatcaag ctgatcgccg aggtcaaaaa ggcgagcccg agcaaaggcg
     4501 ttatccgggc agacttcgat ccggtagcga tcggacggac gtatgaggct cacggtgcga
     4561 tgtgcctgag catcctcacg gacgagccct attttcaggg cagcctcgaa tatctgaaac
     4621 aagtgcggca ggcggtggcc attccagtgt tgcggaagga tttcattctc gattcgtacc
     4681 agcttaccga agcccgcgcg gcgggcgccg atgcggtgct gctgattgcg gagtgcctgg
     4741 acgactgcca cctgcggaaa ttgcacaacg aagccctgga gctgggaatg acgccgctgg
     4801 tcgagttcta tgagccggcg aacctcgacc gggtgctggc ggcgggggca accttggtgg
     4861 gcgttaataa tcgcgacttg cggacctttg aggtcgatct ggaacatacg atccggatgc
     4921 ggcagcgcgt cccccagggc tgcgttttgg tcggcgaaag cggcatcctt gcgcgagccg
     4981 acgcccttcg actagaatca gcgggagtcg aggcaatgct cgtcggcgaa agcctgatgc
     5041 gcgaagccga tatcggggcc gcggtcgatc gactgctcgg cgtcagcgtc tagtcgtgcg
     5101 gagatgaaat atggccggaa tgatggaagg gctttcgacc gtcaacagct ttctccgcac
     5161 aatggtggcg attgtggtgc tcggcgcggt gggtgtcggc ggatggttcg gctatacgac
     5221 tttcaacgcc
//
LOCUS       DASUGB010001060         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_952382, whole genome shotgun sequence.
ACCESSION   DASUGB010001060 DASUGB010000000
VERSION     DASUGB010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952382"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="speA"
                     /locus_tag="VFV87_20105"
     CDS             complement(<1..935)
                     /gene="speA"
                     /locus_tag="VFV87_20105"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX5106139.1"
                     /translation="MLKDPVDRWTVSDASELYDVPRWGKGYFSINAKGHMQVHPTKDP
                     AQAIDLKELIDRLQMRGLDLPILLRFNGILKDRLREMHDVFAQAIKEHDYKGGYICVY
                     PIKVNQQRHVVEQIVNYGRVYGFGLEAGSKPEMLATVAMTEAETPIICNGFKDAEFIE
                     MAMLAQKMGRRVIPVVEKYTELALILKYAEKVGVRPQIGFRIKLAARGSGRWQSSGGY
                     RSKFGLTVGEILHAVEELRSRDMLDCFKLLHFHLGSQITNIRHVKGAMNEAARAYADL
                     VKRGCGLEYLDVGGGLGVDYDGSQTNFESSVNYTL"
     gene            complement(1143..1718)
                     /locus_tag="VFV87_20110"
     CDS             complement(1143..1718)
                     /locus_tag="VFV87_20110"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HEX5106140.1"
                     /translation="MFTLRQPTEQRIRDYLSAQVSQAFSYDDVGCTRGEPAAKRGWNI
                     DRQRVLLGRGREVFEAAKQAISNWRMFPQEVARVCWPEPPQTGLVVGVLYKVWLLPVW
                     ILFPARVVWVMDEQVGEIHRFGFAYGTLPDHPERGEERFVVEWNDADDSVWYDLLAVS
                     QPTHWLARIGYPYTRYEQARFRRLSAAAMRK"
     gene            complement(1954..2232)
                     /locus_tag="VFV87_20115"
     CDS             complement(1954..2232)
                     /locus_tag="VFV87_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106141.1"
                     /translation="MRKSSGLTVVVVAAILLLMLAPLLYVAAIGPVVWLADRGMIDVD
                     EHSIAAKVYAPLEWSANECEPVAGALEWYVQLWSAPPASPPAEQYHSY"
     gene            2383..3006
                     /locus_tag="VFV87_20120"
     CDS             2383..3006
                     /locus_tag="VFV87_20120"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HEX5106142.1"
                     /translation="MNILQPPNAIFLLGLVVQFLIRFYFARRTKAEKKAVRQIDGMER
                     LLLGLMFPAVLLLPPLYCFTPLLSFADYELPQPVRWAGAVVMVLSLWLFWRSHVDLGQ
                     NWSVSLEIREHHQLVTCGVYRWMRHPMYASIWLWGLAQGLMLNNWLAGWAVIPAFAAM
                     YFLRTPREERLMEQQFGDSYRDYARRTGRIFPRFTKAAPEPEVTPRP"
     gene            complement(3055..>3469)
                     /locus_tag="VFV87_20125"
     CDS             complement(3055..>3469)
                     /locus_tag="VFV87_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106143.1"
                     /translation="SGQADSLVSIEIYSEEQIRIASDARTMNLEELGKKYQHVGEQAA
                     KALAEYKASLEAQRERNSRFDFPPQGEPVVSLGEDRTVLLCTVAHVGDDYVLVSYGDE
                     NPRKQVFAKQAIARIRWATGDLRFRTAVRRNENRE"
BASE COUNT          604 a         1145 c         1011 g          709 t
ORIGIN      
        1 tgcagcgtgt agttgacgct cgattcgaag ttcgtctgcg agccgtcata atcgacgccg
       61 aggccgccgc ccacgtcgag atattccaac ccgcagccgc gtttgaccag atcggcatag
      121 gcgcgagccg cctcgttcat cgcccctttc acgtggcgga tgttcgtgat ctggctgccg
      181 aggtggaagt gcagcagctt gaagcagtcg agcatgtcgc ggctgcggag ctcttcgacc
      241 gcgtgcagga tttcgccgac ggtcagcccg aatttcgagc gataaccgcc cgacgactgc
      301 caccgcccac ttcctcgcgc cgcgagcttg atccggaagc cgatctgcgg ccgcacgccg
      361 accttttcgg catatttgag gatcagggcc aactcggtgt atttctcgac gaccggaatg
      421 acgcggcggc ccatcttctg ggccagcatc gccatctcga tgaactcggc gtccttaaag
      481 ccgttgcaga tgatcggcgt ctccgcttcg gtcatggcga cggtcgccag catttcgggc
      541 ttgctgcccg cttccaggcc gaagccgtag acgcggccgt agttgacgat ctgctcgacg
      601 acgtgccgct gctggttgac cttgatcgga tagacgcaga tgtagccgcc cttgtagtcg
      661 tgctctttga tcgcctgagc gaagacgtcg tgcatttcgc ggaggcggtc tttcaggatg
      721 ccgttgaacc gcaacaggat cggcaggtcg aggccccgca tctgcagccg gtcgatcagc
      781 tccttcaggt cgatcgcctg ggcggggtcc ttcgtgggat ggacctgcat gtgccccttg
      841 gcgttgatcg agaaatagcc tttgccccac cgcggcacgt catacagttc gctggcgtcg
      901 gacaccgtcc agcgatcgac cgggtctttg agcatttcgt gaaggtcctg ttcgcgggag
      961 gcgcaggttc gcggcgcgcg gagagtaaaa actcaccatt atacgcatcg tggcgcgagg
     1021 gcaagggtcc gtagggttct cgcccgcacc gtaggcctct cgctccgtga gaggcaaccg
     1081 actttccatg cgcaaagacc ctcgcgcata gagagtctcc cgcgactcgc ggagcgagtc
     1141 gcctactttc gcatcgctgc ggccgacaag cgacggaacc gcgcctgctc gtagcgcgtg
     1201 tacggatagc cgatccgggc cagccaatgg gtgggctgcg aaaccgccaa gaggtcgtac
     1261 cacacgctgt cgtccgcgtc gttccactcc accacaaacc gctcttcacc ccgctccgga
     1321 tggtccggca gcgttccata cgcaaagccg aagcggtgaa tttcgccgac ttgctcgtcc
     1381 atcacccaca caacccgcgc cggaaacaaa atccacaccg gcagcagcca gactttgtaa
     1441 agcacgccca cgacgaggcc cgtctggggc ggctcgggcc agcagacccg ggccacctcc
     1501 tgcgggaaca tccgccaatt cgagattgcc tgtttggccg cttcgaacac ttcccgtccg
     1561 cggcccaaga ggacgcgctg gcggtcgata ttccaccccc gtttggccgc cggctcgcct
     1621 cgcgtgcagc cgacgtcgtc gtacgagaac gcttgactta cctgcgcgga cagatagtcc
     1681 cgaattcgtt gctcggtcgg ctggcggagt gtaaacatga gcgtcggtct gctttcctac
     1741 cgcgaaacgg acctccttgc taaccggtcg cggccatttc ggccgcatgc ttcttgagct
     1801 tatctgctga ccgcctgaat gagcgatgcg cggaaaggac caggggtgtc cattccgttg
     1861 tcgcgcgcga aaagaactta cgacgacgta ccccgccgta agttcacgcg atccgggaag
     1921 cagcgcctgg atcgagcgcg tccacaccct tactcagtaa gagtgatatt gctctgcggg
     1981 tggagaggcg gggggggctg accagagctg gacataccac tccaatgccc cggctaccgg
     2041 ctcgcattca ttggccgacc attccagcgg tgcatagacc ttggcggcga tcgaatgctc
     2101 gtccacgtcg atcattcccc gatcggccag ccagacaacc gggccgatcg ccgcaacata
     2161 gagcagcggc gccagcatca gcaacagaat cgccgccacc accacgaccg tcaaaccact
     2221 ggattttctc atccctgatt ccctccagcc gcggattatc ctgccgttcg cccgaggcgg
     2281 tcaacggcgc ttttccgccg gactgcctcc tatggtggga attggggcgt tctatttttg
     2341 gcaagccggc gaaccattcc gtagaatctc cctcactcgt ccatgaacat ccttcagcct
     2401 ccgaatgcca tcttccttct ggggcttgtc gtccagttcc tgatccgttt ctacttcgcc
     2461 cggcgcacga aggcggaaaa gaaggcggtc cggcagattg acgggatgga acgcctgctg
     2521 ctcgggttga tgtttccggc ggtgctcctg ttgccgccgc tctactgctt cacgccgctg
     2581 ctttccttcg ccgattatga attgccgcag ccggtccgct gggccggcgc ggtggtgatg
     2641 gtcctctcgc tgtggctgtt ttggcgctcg cacgtcgatc tggggcagaa ctggtcggtc
     2701 agcctggaaa tccgcgagca ccatcaactg gtcacttgcg gcgtctatcg ctggatgcgg
     2761 caccccatgt acgcctcgat ctggctctgg gggctcgctc aggggctgat gctcaacaac
     2821 tggctcgccg gctgggcggt cattcccgcc tttgccgcga tgtacttcct ccgcacgccg
     2881 cgcgaagagc ggctgatgga gcagcaattc ggcgacagct accgcgacta cgcccgccgc
     2941 accggccgca tcttcccccg tttcaccaag gccgcaccag agccggaggt tacgccgcgt
     3001 ccgtagtgca gaatcagcgc tccaccgagc caactcggtg cggctccatc ggaattattc
     3061 ccgattctcg ttgcgtcgca cggcggtgcg gaatcgcaaa tcgcctgttg cccagcgaat
     3121 gcgggcgatc gcctgcttgg caaacacttg tttcctcggg ttttcgtcgc cgtaactgac
     3181 cagcacgtag tcgtcgccaa cgtgcgccac cgtacagagc agcaccgtcc ggtcttctcc
     3241 caaggagacg accggctccc cttgcggcgg aaagtcgaac ctcgagtttc gctcccgttg
     3301 agcttccaga gatgccttgt actccgcgag tgctttcgcg gcctgctcgc caacgtgttg
     3361 atatttcttc cccagttcct ccaaattcat cgtgcgcgcg tcgctcgcga tccgaatctg
     3421 ctcttcgctg taaatctcga tggaaacgag ggaatctgcc tgtcccgac
//
LOCUS       DASUGB010001061         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_536037, whole genome shotgun sequence.
ACCESSION   DASUGB010001061 DASUGB010000000
VERSION     DASUGB010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536037"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1005
                     /locus_tag="VFV87_20130"
     CDS             199..1005
                     /locus_tag="VFV87_20130"
                     /inference="COORDINATES: protein motif:HMM:NF010309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase family
                     protein"
                     /protein_id="HEX5106144.1"
                     /translation="MDDPTTEQANAVLGVVDSVMGDSAVAVYLYGSAVVGGLMPASDL
                     DLFAVTSRRTAPAERWDLVTRLIPISRRGQRPSSWRPVELTVVALPDVQPWGFPPRTD
                     FQYGEWLRDRFDAGSADPEHPDNPDLALLIEQVRRTGQTLVGQPPSEVLPVIPESDLR
                     AALVGVIPGLMSDVDSDTTNVLLTLARVLYSLETGEFTSKDAASDWAITRLDSRHVLP
                     LQKAKDIYLGRRADDWSEDIDLARAAAASLALNTIETHVEQVPRRRDLRD"
     gene            965..1339
                     /locus_tag="VFV87_20135"
     CDS             965..1339
                     /locus_tag="VFV87_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106145.1"
                     /translation="MSSRFRGGEIYGIEISTVFDENNAIRCEGCGEPIGGTPFRISLL
                     DIVAPESPPSWAVGARLNPGPHQFHAEPEHFRSWARGRGHYFCRLSDVREIMRPVPLP
                     GDTARWGLCDGLHREAHEFIPA"
     gene            1336..>2387
                     /locus_tag="VFV87_20140"
     CDS             1336..>2387
                     /locus_tag="VFV87_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106146.1"
                     /translation="MTVLLPGITALLALAFSAAVFDQWLRKRHAFQLIWAAGMLFFAV
                     ASGCETIAAASGWNEALYKTWYLTGAVWTAGWLGLGTCFLLGRTRFGYAVALSLFLAG
                     LFTFLAARRMTEPAGLAPILYFIAAGVLAAAVAIETYFQNERWPSIAAVAVIGATLTS
                     IVLMILATVPPPGYALDPRTGAPIGDLFPPAIRLLTPFMNITGTFALVLGALFSAYVF
                     MPKKRVLNYSLDPGQSGDSFLFNLAIAPVAITVNFFASLPTAIRDLFAGKLHSRVPAT
                     LLIALGALVVGSGDTFARLGSTDFFQLGKLIGVILLFLGFLVSIEVFNDIRIPFTNRV
                     LRSGRHEPLAAISAQSG"
BASE COUNT          357 a          815 c          739 g          476 t
ORIGIN      
        1 caaaacggcg gctcctgggt gctgcgagtc agcaccgcca acgcgacttc atcgatcttc
       61 ttccctcagc cgggctccta cgagttgcga acgcgcatgc gccagcagct cagcggccag
      121 gtaagcggct acagcccgat caccaagatc accgtctact agccgctcac tcgctcagct
      181 ctcggctagg ctgggccgat agacgatcca accaccgagc aagcaaacgc agttctgggc
      241 gtggtcgata gcgtcatggg cgacagtgcc gtggccgtct atctgtatgg ctccgccgtc
      301 gtgggcggct tgatgcccgc gagcgatctc gaccttttcg cggtcacgtc acggcggacg
      361 gcgcccgccg agcgctggga cctggtgacg cgactcatcc cgatctccag acgcggccag
      421 cggccttcca gttggcggcc agttgagctg acggtcgtcg ctttgcccga cgttcagcca
      481 tggggctttc cgccgcggac ggatttccag tacggcgaat ggttgcgcga ccggttcgac
      541 gccggcagcg ctgatcctga gcatccggac aaccccgacc ttgcgctgct gatcgagcag
      601 gttcggcgga ctgggcagac actcgtcggg cagccacctt ccgaagtctt gccggtgatc
      661 cctgagtcgg atcttcgggc ggcccttgtg ggcgtgattc ctggcttgat gtcggatgtc
      721 gactccgaca ccacgaacgt gctgctgact ctcgcccggg ttctttactc gctcgaaacc
      781 ggcgagttca cgtccaagga tgctgcgtcc gactgggcga tcacgcgatt ggactcacgc
      841 cacgtgctgc cactgcagaa ggcgaaggac atctacctgg gtcgccgcgc ggatgactgg
      901 agcgaagaca tcgatttggc gcgggcagca gcggcatcgc tggcactgaa tacaatcgag
      961 acgcatgtcg agcaggttcc gcggcggaga gatctacggg attgagattt cgaccgtctt
     1021 tgacgagaac aacgcgatcc gttgcgaggg ctgcggtgag ccgatcggcg gcacgccgtt
     1081 ccgcatcagc ctgctcgata tcgtcgcgcc agagtcgccg ccatcgtggg cagtcggtgc
     1141 gaggctgaac ccggggccgc accagtttca cgctgagccc gagcactttc ggagctgggc
     1201 gcgtggacgc ggccactact tctgccgcct ttcggacgtg cgcgagatca tgcgccccgt
     1261 accgctaccg ggcgacacgg cccgctgggg cctgtgcgat gggcttcatc gggaggcaca
     1321 cgagttcatt ccggcatgac tgtcctcctc ccgggaataa ccgccctgct cgcgctcgcc
     1381 ttctctgccg ccgtcttcga tcagtggctc cgcaaacgcc acgccttcca gctcatctgg
     1441 gccgcgggca tgctgttctt cgcggtcgcg tccggttgcg agaccatcgc tgcggcgagc
     1501 ggctggaatg aggcgctcta caagacctgg tatctgactg gcgcggtgtg gaccgctggc
     1561 tggctgggcc tgggcacgtg cttcctgctc ggccgcacgc gctttggcta tgcggtggcg
     1621 ttgtcgctgt tcctcgccgg cctcttcacg ttcctggccg cccgacgcat gaccgagccg
     1681 gccggccttg ctccgatcct gtacttcatc gccgccggcg tgctcgccgc ggccgttgcc
     1741 atcgagacgt atttccagaa cgagcgctgg ccgtcgattg ccgctgtcgc cgttatcggg
     1801 gccacgctga ccagcatcgt gctcatgatc ctcgccacag ttccgccgcc ggggtatgca
     1861 ctcgatccgc gcaccggtgc gcctatcggc gatctgttcc cgcccgcgat acggctgctg
     1921 acgccgttca tgaacatcac cggcactttc gcgctggttc ttggcgcgct cttctccgcg
     1981 tacgtgttca tgcccaagaa acgcgtcctc aactattccc tggatccggg ccagtcgggc
     2041 gattcgttcc tgttcaacct ggccatcgcg ccggtggcaa tcacggtcaa cttcttcgct
     2101 tcgctgccca cagcgatccg cgacctgttt gcgggcaaac tccacagccg cgtgcccgcc
     2161 acgctgctga tcgccctcgg cgctctcgtc gtgggcagcg gcgacacgtt cgcgcgcctg
     2221 ggctcgaccg acttcttcca gctcggcaag ctcatcggcg tgatcctgct cttcctgggc
     2281 ttcctggtct cgatcgaggt cttcaacgac atccgtattc cattcacgaa ccgggtgctg
     2341 cgcagcgggc gccacgaacc gctggctgcg atctccgcac agagcgg
//
LOCUS       DASUGB010001062         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_536229, whole genome shotgun sequence.
ACCESSION   DASUGB010001062 DASUGB010000000
VERSION     DASUGB010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536229"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1129)
                     /locus_tag="VFV87_20145"
     CDS             complement(233..1129)
                     /locus_tag="VFV87_20145"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5106147.1"
                     /translation="MLRTFSILCAGLAALSFLTGSAVAQQEAPAKPAAKGKVEVATTF
                     RSGDLIGMKVKNRAGENLGQIEDLVVELKSGDIRYAALSFGGFAGFGDKLFAVPWQAM
                     TFKFGENDRFFVFDVTQKELEAAPGFDRDRWPDVGNPEWSEGIDKHYSSKRTAAVDQA
                     PRPAPSNELKEADASPADAIFRVSTIDGMNVRNDAGKDIGHVEELVLDIKSGKVKYAA
                     LSFGGFVGFGDKLFAIPMRAFTLKHGADETFFVLDISQDRLEKAPGFDKNNWPDTADP
                     QWSAEIDKYYPERTAQRPTTRK"
     gene            complement(1433..2815)
                     /locus_tag="VFV87_20150"
     CDS             complement(1433..2815)
                     /locus_tag="VFV87_20150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5106148.1"
                     /translation="MKTKRKILVVEDKQHERLAIARLLWQEDYDVEMAENAQQAIALT
                     EGAFDLVISDLRMGENSGIDLLRAWKKKRPSTPFVLITAHGDVGSAVEAMKLGAEDYL
                     TKPVNPDELLILISRCIESRRKDETIRQLQERLDDRLGFEKIIGTSKAILSVFEQARM
                     AAQADCTVLITGESGTGKELIAEALHHNSPRKSGPFVTINMAAVPEHLAESELFGHVK
                     GAFTGAMSARVGRFEAADGGTLFIDEIGDFALPSQAKLLRVLENHVVTPVGSNDDKQV
                     DVRAVAATSRCLETMVRDSKFREDLYYRLNVVTIHLPALRERREDIPLLAAYFLKTFC
                     ETHGKPALEVDQSLMEYLQSYNWPGNVRQLRNTIESMVVLTAGNRLTPDNLPPTFDGD
                     PLAVSNRVFPVSTTSLVDLQRAAVEKALADCNGHRTRAAETLGISVRTLQRKLKAWGM
                     EEKERVEAPT"
     gene            complement(2812..4557)
                     /locus_tag="VFV87_20155"
     CDS             complement(2812..4557)
                     /locus_tag="VFV87_20155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5106149.1"
                     /translation="MVIAARSLPYLNATTCRREKLRNCFLPLTFLRLVACQKCFLIRD
                     LRQNLRVSQKAVAGATMCRMATTCRTMKAVWFHRWRSFSARNLRRWMSGGLPLPGLNG
                     AKSQRGMRLFDQAMASPYQPSNLRRSWAPHLLIALPLLLLFINCLRGVYYDVQMIRTV
                     ALRGEMSRLHSQARYRVARIENLLEAHAENESWSTLREQPWMRNYWTSIQTPESQRLY
                     VAIVDPTGRIVLHSDPKQEGNYLAPEWYDRRVNEAGTEVVFASTSPLSEHNPAYDLRV
                     PIGLPNRPMGALHVGLSAAWLNNNVAGLERQTLVGWAWMLALAVVCSIAAVYGLSELS
                     RQRIELQRKVREGASSRSRELAQLGCGLAHEIRNPLHALRINLHTLKRAINNGTQFSR
                     DQMTAMVSDSDAVIDRLDVLLHDLVQFTSPPQGERTRLDVVREVRATLNLMSGDLNRQ
                     HVKVQTEFPAAPVMVAAEAACFRQALLNLLTFAQQGAGQDGTIDVAVRVNRSRAEIAV
                     ANRGLTLDDEQLAQMFDPFQGPAEAGSGLGLALVRCLFEQFGGSVGGESPVSGGTVIS
                     VRLPLAESRDAGVTK"
     gene            complement(4579..>4921)
                     /locus_tag="VFV87_20160"
     CDS             complement(4579..>4921)
                     /locus_tag="VFV87_20160"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5106150.1"
                     /translation="ARNQASNADVMKVLGGIVGGIGFLGAGAILRSRADIHGLTTAAT
                     IWIVAAIGVACGLGLYFLAATSLGLAIVILLGFGFVERIYFEPVSRPGSADSRASKAD
                     GAKSADTGEVG"
BASE COUNT          976 a         1490 c         1455 g         1000 t
ORIGIN      
        1 ggcggcttcg atctcatcat cggtgaggtc accccatttc tcgcgcagct tgccgctgaa
       61 ctgcttccag tcgcccttca gagtgtccca gttcatgatt tcctcccagg tacggtgcgt
      121 aagtccatct tcagaaaacg ccctggcagc acgcgactcc tcaacaatga gcagctacga
      181 ctaccagggc gggtgggtct cattcagccg ggcattgcgc tcggctgctg ttttatttgc
      241 gagtcgtcgg gcgttgcgcc gtccgctcgg gatagtactt gtcgatctcc gcggaccatt
      301 gcggatcggc ggtatcgggc cagttgttct tgtcaaagcc gggcgccttt tccagcctgt
      361 cttggctgat atccagaaca aagaaggttt catccgcgcc gtgtttgagc gtaaaagcgc
      421 gcatcgggat ggcgaacagc ttgtcgccga atcccacgaa tccgccaaac gacagcgcgg
      481 cgtacttcac cttgccggac ttgatatcga ggacgagttc ctcgacgtgg ccaatgtctt
      541 tgccggcatc attccgcacg ttcatgccgt cgatggtgct gacgcgaaag atggcgtcgg
      601 caggggacgc atcggcctcc tttagttcgt tgctaggggc gggcctcggc gcttgatcga
      661 ccgcagccgt tcgcttgctg ctgtagtgtt tgtcgatccc ttcgctccat tccggattgc
      721 cgacatcggg ccagcgatcc cgatcaaagc ccggcgccgc ctccagctcc ttttgcgtca
      781 catcgaacac gaaaaagcgg tcgttttcac cgaatttgaa ggtcatcgcc tgccacggca
      841 cggcgaagag tttgtcgccg aagccggcaa agccgccgaa cgacagagcg gcatagcgga
      901 tgtcgcccga tttgagttcc acgaccagat cctcaatttg ccccagattt tcaccggcgc
      961 ggttcttgac cttcatgccg atgaggtcac cggatcggaa agtcgtggcg acttccactt
     1021 ttcccttggc agcgggcttg gccggcgctt cttgttgcgc gacagccgag cccgtcagaa
     1081 aggataaagc tgcaagtccg gcacacagga ttgaaaatgt tcgcaacatg tctctgctcc
     1141 ttgtactacg aagtcgttgc gcgcttcgcc ggcgtcacgc gccgcccaac gaagcgcatg
     1201 gtgacacggt tgctcgatgc atttagcgat tgctatcggt ctaaagctga agcaaacgga
     1261 gtgccggcca cctgtcctgt tgagcgtctg tggcagaagg caaattcgcc ccgaaaatcg
     1321 cggcggaacc tttgggaaga agaagtatgc gcgtgcgttc ggttccgaat tcgggggcca
     1381 gaaccgacaa attgccgcgt tggccagacg ccctcgtccg gctgtcggag gactaggttg
     1441 gcgcttcgac gcgctccttc tcctccatcc cccaggcttt gagcttgcgc tgcagcgtgc
     1501 ggacggaaat gccaagcgtt tccgctgccc gcgtgcgatg gccgttgcaa tcggccaggg
     1561 ccttttcgac ggcggcccgt tgcaaatcca ccagtgaagt ggtggagacc ggaaatacac
     1621 gattcgacac cgccaacgga tcgccgtcga acgtgggcgg cagattgtcg ggcgtcagcc
     1681 ggtttccggc ggtgaggacg accatgctct cgatggtgtt ccgaagttgg cgcacgtttc
     1741 ccggccagtt gtatgactgc agatactcca tcaggctttg atcgacttca agcgcgggct
     1801 tgccgtgggt ttcgcagaac gttttcagga agtaggccgc caatagcggg atatcctcgc
     1861 gtcgctcgcg aagggcaggg agatgaatcg tcaccacatt caaccgataa tagagatcct
     1921 cgcgaaattt actgtcccgg accatcgtct cgaggcagcg gctggtggcg gcgacggccc
     1981 gcacgtcgac ctgtttgtcg tcgttgctgc cgacgggcgt gacgacgtgg ttttcgagca
     2041 cgcgaagcag cttcgcctgg gaagggagag cgaaatcgcc gatttcgtcg atgaacagcg
     2101 tcccgccgtc ggcggcttcg aagcggccga cgcgggcgga catcgcgccg gtgaaggccc
     2161 ctttgacgtg gccgaagagc tcgctttccg ccaggtgttc cggaacggca gccatattga
     2221 tggtgacaaa gggcccgctc ttacgcggac tattgtgatg cagcgcttcc gcgatgagtt
     2281 ctttgccggt gcccgactcg ccagtgatca ggaccgtgca gtcggcctgg gccgccatgc
     2341 gagcctgctc aaagaccgag agaatcgcct tggacgtgcc gataatcttc tcaaagccca
     2401 agcggtcgtc gagccgctcc tgaagctggc ggatcgtttc atccttgcgg cgcgattcga
     2461 tgcagcggga gatcaggatc agcaactcat cggggttcac gggcttggtg aggtagtctt
     2521 cggccccgag cttcatcgcc tcgactgccg agcccacgtc gccatgggcg gtaatcagca
     2581 cgaaaggagt gcttggccgt ttcttcttcc aggcccgcaa cagatcgatg ccgctgtttt
     2641 cgcccatccg caagtcgctg atgacaaggt caaacgcacc ctccgtcagg gcgatcgctt
     2701 gctgggcgtt ttctgccatt tcgacgtcgt agtcttcctg ccagagcagg cgggcaatcg
     2761 ccagccgctc gtgctgcttg tcttcgacca ccaggatctt ccgtttcgtt ttcatttggt
     2821 tactcctgca tcgcgggact cggccagggg gaggcgaacg ctgatgacgg taccgccgct
     2881 caccgggctt tcgccgccaa ccgaaccgcc gaattgttcg aacagacatc gcaccagcgc
     2941 caatccgagc ccggagcccg cttcggcggg cccttgaaac gggtcgaaca tctgggcgag
     3001 ttgttcatca tcaagcgtca aaccgcgatt ggcgaccgcg atttccgcgc gactgcgatt
     3061 cacgcgcacg gcgacatcga tcgttccatc ctggccggcg ccttgctggg cgaatgtcaa
     3121 cagattcaac agcgcctgac gaaagcacgc ggcctccgcc gcgaccatca cgggcgctgc
     3181 cggaaattcc gtttgcactt tcacgtgctg acggttcaag tcaccggaca tcaggttcag
     3241 cgtggcgcgg acttcccgca caacatcgag ccgagtgcgc tctccctgcg ggggcgaagt
     3301 gaactgcacg aggtcgtgca gcaagacgtc gaggcgatcg atgaccgcat ccgaatccga
     3361 aaccatggcc gtcatctgat cgcgactgaa ctgcgtgccg ttattgatgg cccgcttcaa
     3421 ggtgtggagg ttaattcgca aggcatggag tggattgcga atctcgtgag ccagaccaca
     3481 gccaagttgc gccaactcgc gactgcggga actggctccc tcgcggacct ttcgctgcaa
     3541 ttcaatccgt tgccgggaca actcagacag gccatagacg gcggcaatgc tgcacacgac
     3601 agccagggcc agcatccacg cccagccgac gagcgtctgc cgctcgaggc cagccacgtt
     3661 gttgttcagc cacgcggcgc tcaggccaac gtgcaaagcg cccatcgggc gattgggaag
     3721 cccaatcggc acgcgcaggt cataagccgg attgtgctca gaaagcgggc tggtgctggc
     3781 aaacacgacc tcggtgccgg cttcgttcac gcgacgatca taccattccg gcgccaaata
     3841 gtttccttcc tgtttggggt cgctgtgcag cacgatgcgt cccgtgggat caacgattgc
     3901 cacgtagaga cgctgcgatt cgggagtctg aatgctcgtc caataatttc gcatccaggg
     3961 ctgttcgcgg agcgtggacc acgactcgtt ttccgcatgt gcctccagga ggttctcaat
     4021 tcgggcgacg cgataccgcg cctgcgaatg gagacggctc atttcaccgc gcagagcgac
     4081 ggttcggatc atctgtacgt cataatacac accacgtaag caattgataa aaagcaagag
     4141 cagcggcaat gcaatgagca gatggggcgc ccaggaccgg cgcagattcg aaggttgata
     4201 aggagaagcc atggcctggt caaacaacct catcccccgc tgcgacttgg cgccgtttag
     4261 gccgggcaaa gggagacctc cactcatcca acgtcgcagg tttctcgcgc tgaacgagcg
     4321 ccatcgatgg aaccaaacag ccttcattgt acgacatgtt gtcgccatgc gacacattgt
     4381 cgcacctgcg acagctttct ggcttacgcg tagattctga cgtaagtctc ttattaagaa
     4441 gcacttctga cacgcaacga gacggagaaa cgtgagaggc aggaagcaat tgcgaagctt
     4501 ttctcgacgg caagttgtcg cgttgagata tggcaaactg cgcgccgcga taaccaaata
     4561 ggggtcgcaa gcaagcgatt acccgacttc gccggtgtca gcagattttg cgccgtcggc
     4621 cttgctggcc ctggaatctg cggagccggg ccgggacact ggctcgaagt aaatacgctc
     4681 cacaaaacca aacccgagta aaatgacaat tgccaacccg aggctcgtcg cagccagaaa
     4741 atagaggccc aggccgcagg ccacgccgat tgctgcgaca atccagatgg tggcggccgt
     4801 ggtcagccca tggatatcgg cacgcgaacg caagatcgcc ccggcgccta agaagccgat
     4861 gccgccgacg attcccccca ggaccttcat tacgtccgcg ttgctcgcct gatttcgcgc
     4921 g
//
LOCUS       DASUGB010001063         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2063808, whole genome shotgun sequence.
ACCESSION   DASUGB010001063 DASUGB010000000
VERSION     DASUGB010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063808"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..3094
                     /locus_tag="VFV87_20165"
     CDS             290..3094
                     /locus_tag="VFV87_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106151.1"
                     /translation="MRVQAGALVEDPLILICSTDWDRKWARLIDEHLSALGLGGSRRV
                     HLMPDAAGMVSQRAAEAIDTARLAVLVITPDYLASRHLVREVLPRLVLRASMVIKHGQ
                     TGDIEFLPIIAKECQWGQLPETVCVQVTPELKTPLELRENREVHRVLFQAARKAQEYL
                     GRLKILPVDTAWKRPRDSAVRLGALEPITGRDFQDREKSLHELNVAWMRPETRVINVV
                     APPGAGKTTLFRQWLQHLSRDNFRGAERVFAWSFDRQTRAGRAATADEFLRRAGEFLV
                     LSDEAAMPYWQRGADLAKEFQRQRSLVVLDGVDPLLETSEVGQAALRDLGLYFFLRKW
                     LAGDQGLCVVASSRPLADFASWQGKALETSELDPLTPVEAVELIQKMKVPGGEGALRE
                     AVECAGGHTLSTRLLGTWLAANHNQASGRTADTSNFLLIPRDHLASSFISAYAHLVGN
                     GPELRVLRLLALADQPVELELFQTLISGEPISGITDGLAELDREALGGVLARLRWAGL
                     MEPPVGETLDLPPLVRKHFRAEVESQLQPGWQAVQARFFALLRRPPQEPPTSFRELLT
                     ALRAFRHGCLAAAGSEAFRQVLLPQLWGTEDELRFTPTRQLCTRLEALAALLNEATEL
                     PQGELDPADRQAVFQLWPRSLRAAGRFEDAAAAAERQMLWARNHGHLDEMGNCLLELT
                     EILLLQGKLKEATRRVNELFVFPDIRDNAQLFRDTVLLSARILQLKGQFKDALNRYEL
                     AKSSPATTSRLLGAEYWHCESILDSGDFLKAERTAWRQFEELADKNGLAAAGFALVLV
                     KMYFSLRTHSGDWRYREEAGKHLAAARHASLRAGMVEGMAPVELAAAALHRHEGEFPE
                     ATEALIDATALVSRLGLKFLQLECQLEAIRLKLDQGQGRGVPERLEMLRQHVQAALYG
                     RVARDFKQIESRLVVVKRPV"
     gene            complement(3688..>4078)
                     /locus_tag="VFV87_20170"
     CDS             complement(3688..>4078)
                     /locus_tag="VFV87_20170"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5106152.1"
                     /translation="VPQLIATLRGDDDEAKEMAADALGVLGPAADEAADVLRPLLASE
                     AEGVADWSAIALGKIAGTDEALPRLMTIIRERQGRNIRVQAAEALGNIGPKARTALPL
                     LHSALDDPDEDFRHAVEGAIAFIEDEV"
BASE COUNT          694 a         1446 c         1219 g          719 t
ORIGIN      
        1 agggcagcct cctttagccc ccgctgaggg gcgctggccc gtggggcgac agccctcatt
       61 ttagacggag cggccgacaa tgccagtcgc gcgtcgccac gctatccaga acgcgggaat
      121 gccagcagtc ttcgccgcgc actgcggagt gcggctacga ttctgccagc ggcttgccgc
      181 aatcatccgc cggcctccga tccaacccaa ctgtttaacc aattcttcgt attcgtttgt
      241 tagacccgtt cggttcggaa atgcaccgcc acaacgcacg caccgcagga tgcgggtcca
      301 ggcaggagcg ctcgtggaag acccgctcat actcatctgc tcgaccgact gggaccgcaa
      361 gtgggcccgc ctcatcgacg agcacctctc ggccctcggg ctggggggct cgcggcgggt
      421 ccacctgatg cccgatgctg ccggcatggt gtcgcagcgg gccgccgaag ccatcgacac
      481 cgcccggctg gcggtgctgg tcatcacgcc cgactacctg gcctccaggc acctggtgcg
      541 agaagtgctc ccccggctcg tcctgcgggc cagcatggtc atcaagcacg ggcagaccgg
      601 cgatatcgag ttcctgccaa tcatcgccaa ggaatgccag tggggccaac tgccggaaac
      661 cgtctgcgtg caggtcacgc ccgaactgaa gacgcccctg gagttgcgtg agaaccgcga
      721 ggttcaccgc gtactcttcc aggccgcccg caaagcccag gaatatctcg gccgcctcaa
      781 aatcctgccc gtcgatacgg cttggaagcg gccgcgcgat tccgccgttc gcctcggcgc
      841 tctggagccg atcacgggtc gcgactttca ggatcgcgaa aaatcactgc atgagctgaa
      901 cgtcgcctgg atgcgtccgg aaacgcgcgt tatcaatgtc gtcgctccgc ccggcgccgg
      961 caaaaccacg ctgttccgcc aatggctgca gcacttgtcg cgcgataatt tccgcggggc
     1021 agagcgggtc tttgcctggt cgttcgacag gcagacgcgc gccggccgcg ccgccacagc
     1081 cgatgagttc ctccgccggg ccggcgaatt tctcgtcctc agcgacgaag cggccatgcc
     1141 ctactggcag cgcggcgcgg acctggccaa ggagttccag cgccagcgca gcctggtcgt
     1201 gctcgatggc gtcgatccgc tgctggagac gagcgaggtc ggccaggcgg cgcttcgcga
     1261 cctgggcctg tacttctttc ttcgcaagtg gctggccggc gatcaaggac tgtgcgtcgt
     1321 cgcctccagc cggccgctgg ccgatttcgc ctcctggcag gggaaagcgc tggaaacgtc
     1381 ggagctcgat ccgctcactc ctgtcgaagc cgtcgagctg attcagaaaa tgaaggttcc
     1441 cggcggcgag ggcgccctcc gcgaagcggt cgaatgtgcc ggcgggcaca ccctttcgac
     1501 gcgcctgctc ggaacgtggc tggccgccaa ccacaaccag gcgagcggcc gcaccgccga
     1561 caccagcaat ttcctgctca ttccgcgcga ccacctggcc agctcgttca tctccgcgta
     1621 tgcgcacctc gttgggaacg gtcccgaact ccgcgtcctc cgcctcctcg cactcgccga
     1681 tcagccggtc gagctggagc tgttccaaac gctgatttcc ggcgagccga tctctggaat
     1741 caccgacggc cttgccgaat tggaccgtga agcgttgggc ggcgtgctgg ccagactccg
     1801 ctgggcaggg ctgatggagc cccccgtcgg cgaaacgctc gacctgccgc cgctggtccg
     1861 gaagcacttc cgggcggaag tcgagtcgca acttcagccc ggctggcaag ccgtgcaggc
     1921 ccgctttttc gccttgctcc gccgcccgcc gcaggagccg ccaacttcgt tccgcgaatt
     1981 gctcaccgct ctgcgggcct tccgccacgg ctgtctggcc gccgctggca gcgaggcctt
     2041 tcgccaggtt ctcctgccgc agctttgggg gaccgaagac gagctgcgct tcacgcccac
     2101 gcgccagctt tgtactcgcc tggaagccct cgcggcgctc ctcaacgaag cgaccgagct
     2161 gccgcagggc gagctcgatc ctgccgatcg ccaggcggtc tttcaactct ggccgcgctc
     2221 gctccgcgcc gccggccgat tcgaagacgc cgccgctgca gccgagcgcc agatgctctg
     2281 ggcccgcaac cacggacact tggacgaaat gggcaactgc ctgttggaac tgacggagat
     2341 cctgcttctc cagggcaagc tcaaggaagc cacgcgccgc gtgaacgagc tgttcgtctt
     2401 tcccgacatc cgcgacaacg cccagctctt tcgcgacacg gtcctccttt ccgcccgcat
     2461 cctgcagctc aaagggcaat tcaaggacgc cctcaatcga tacgagctgg ccaagtcgtc
     2521 gccggcgacc acctcgcgcc tgctcggcgc ggaatactgg cactgcgagt cgattctcga
     2581 ctccggcgac ttcctgaagg ccgagcgcac cgcctggcgg cagttcgagg agctggccga
     2641 caagaacggc ctggctgccg ccggctttgc tctcgtgctc gtcaaaatgt acttctcact
     2701 tagaacgcac tcgggcgact ggcgctatcg cgaggaggcc ggcaagcacc tggccgccgc
     2761 acgccatgcc agcctgcggg cggggatggt cgaggggatg gccccggtcg agctggcagc
     2821 ggccgccctg catcgccacg aaggcgagtt ccccgaggcg accgaggcgc tgatcgacgc
     2881 caccgccctc gtgtcgcgcc tggggctgaa attcctgcag ctcgaatgcc agctcgaagc
     2941 gatccgcctc aagctcgacc aggggcaggg ccgcggtgtc cccgagcgcc tggaaatgct
     3001 ccgccagcac gtccaagccg ccctctacgg ccgcgtcgcc cgcgacttca agcaaatcga
     3061 aagccgcctc gtcgtcgtca agcgccccgt gtagcgctct tgcgatcttt gcccactcgg
     3121 cgtgccattg ggctggtact cccgacttcc tcctcgaatt cgtcgtgacg tacattacac
     3181 gtcctcctgg taatcccgcc ggtcacttca gcgcaacctc ccgcacaaca cgcaactttt
     3241 taactctttt gacgcgagac ctccccgcgc aaccttatcc taactccagt cccatcagtc
     3301 acttgcgacg cgcaacaccc cccccgcatg aggatgttgc gctgttgcgc gttgtcaatc
     3361 gtttttcctc catttgtcta accgcacctc gcaccggact tgaaggtgat agacatagca
     3421 agcgcgaagt gtaagcaagg gaccgacaac aacccgaagc gtaagcgagg gactcgcgaa
     3481 gcatcggcga gggcatacgc tgccagccac cccgcctgcc ctcccagcgc tcgccctcgt
     3541 ttcggcctcg ctcagggctg cttcccatta tagcaaacgt acaccactcg ccatacccgc
     3601 ttgaggggtc tggcaggcag gaattatctg aattctggtg ccagctgcct cgaatccgcc
     3661 gcgccaatcc gatccgcgac gctgcgtcta aacctcgtcc tcaatgaaag caatggcacc
     3721 ctcgacggca tgccggaagt cttcatccgg gtcatcgagt gcggaatgaa ggagtggcaa
     3781 tgcggtgcgc gccttgggtc cgatgttgcc aagcgcctcg gctgcttgca ctcggatgtt
     3841 ccggccctgc ctttcgcgaa tgatggtcat caggcgcgga agggcctcgt cggtacccgc
     3901 aatctttcct agtgcgatgg ccgaccagtc ggcaacgcct tccgcctcgc tggcgagcaa
     3961 cgggcgaagg acatctgccg cttcgtccgc ggcgggaccg agcacgccca gagcatccgc
     4021 ggccatctct ttggcctcgt cgtcgtcgcc gcgaagcgtc gcaatcaact gcggcaca
//
LOCUS       DASUGB010001064         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2063849, whole genome shotgun sequence.
ACCESSION   DASUGB010001064 DASUGB010000000
VERSION     DASUGB010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063849"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFV87_20175"
     CDS             complement(<1..379)
                     /locus_tag="VFV87_20175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5106153.1"
                     /translation="MTNPNATYDPERHWSTVAEHIAKRDAGSVVAGDDEPLERAKRAK
                     FVAKFLSQLDVQGKTVLEVGCGPGGNLRVLRTQKPRRLVGADVSQAMLDLADHDELVK
                     IDGRSLPFADGEFDVVFSATVLMH"
     gene            complement(854..991)
                     /locus_tag="VFV87_20180"
     CDS             complement(854..991)
                     /locus_tag="VFV87_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106154.1"
                     /translation="MTASLRSLLMWGAAALLAIAIAGSFILWGLNGPAYLVDLIAAYC
                     L"
     gene            complement(988..2235)
                     /locus_tag="VFV87_20185"
     CDS             complement(988..2235)
                     /locus_tag="VFV87_20185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5106155.1"
                     /translation="MTSRSYWTAVWCGFLVLTIGLGVRQSFGIFLKPVSAELHVGREL
                     FSFGTALSMLLMGAVAPFAGRLCDRFGSAPTIAGGGALYVLGMVATATMHDGFMLILG
                     NILVGVGLSAASFGPVLGVILRVAPPAKQALAVGICSAGGSFGQFFIVPLAAVLQSYF
                     GDWRPTMWALTAIAVLMILLSVGLNDSREIAAGRRAGGGRQTTGEALSEAFAQKSYLL
                     LVIGYFVCGFHVAFVGGHLPAYISDKGIGLSLFGINLSPAELGGWAIGMVGLFNIAGA
                     VIWSALGGRHKRKNLLALLYLLRSLVFLGFVLAPLSAASVLIFAGALGFLWLGTVPLT
                     TSLVGYIFGPVHLTMLNGIVFFGHQVGSFFGGWGGGRIFDLQGNYDLMWWISIVLGVV
                     SALLHWPIVEERLTRPVAAAQPA"
     gene            2234..>2585
                     /locus_tag="VFV87_20190"
     CDS             2234..>2585
                     /locus_tag="VFV87_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106156.1"
                     /translation="MAANLRPGGRPDKAFATAFPWNYFMPSQATMWQNMSMARLWFRC
                     GLAVFLLALAGNALAMPRLERERCTFKPPRGNRVECFVLVVPENREQPEGREVRLKVA
                     VLKARRPANAEPLFY"
BASE COUNT          525 a          849 c          833 g          378 t
ORIGIN      
        1 tgtgcatcag tacagtagcg ctgaacacga catcgaactc cccgtccgcg aacggtaacg
       61 accgtccgtc gatctttacg agctcgtcat ggtcggcaag gtcaagcatg gcctgggata
      121 catcggcgcc aaccaggcgg cgcggcttct gggtgcgaag aacccgcaga ttgccacctg
      181 gaccgcaccc aacttcgaga actgtcttgc cctgcacgtc gagttgactg aggaacttgg
      241 cgacaaactt cgctcgtttc gcccgctcga gcggctcgtc gtcgcccgcg acgacgctac
      301 cggcatcgcg tttggcgata tgctcggcga ccgtcgacca gtgacgctcg ggatcgtacg
      361 ttgcgttcgg gttcgtcatg agcgtattcc ttgacttgca gcccttctgg gacgccagac
      421 ggagctgccg aataggccag acaacgcgat cacgcttgac gcttaggctc gggacggagc
      481 caaggcacta ttcacccgag ccggaaactg tatttggtca ggcgctgcaa ccagtgcccg
      541 ctttcactga aagttgactg acattcccca cacacccctg caatggtggg aacgctggca
      601 ttcctgccgg gttccgcgga agccagtaga ttatcttgcg agcctcggtg cggcggcgtc
      661 gatcgtctcg gcgcggcaga ttgctcgatg tcggatatga tctgcgtgcg accccttcca
      721 cgacgcgggt ctgcaccgtc ctctgcggga acacccgccg accgcctcgg ggaagcaagc
      781 cacaccgtcg gtgaccgcaa tcaagcgacg ccacgggccc tcagctcatt cattcgcggg
      841 gaggaatgag aggtcagagg cagtaggccg cgatcagatc caccaggtac gccgggccat
      901 tcagccccca caggatgaac gagccggcaa tcgcgatcgc cagcaaggcg gctgcgcccc
      961 acatcagcag cgagcgcagg ctcgcggtca tgccggttgc gccgccgcaa ccggccgcgt
     1021 gagccgctcc tcgacgatcg gccagtgcag cagggccgac acgacgccaa gcacgatcga
     1081 gatccaccac atcaggtcgt agttgccctg caggtcgaat atccggccgc cgccccagcc
     1141 gccgaagaag ctgccgactt ggtggccgaa gaagacgatg ccgttcagca tggtgagatg
     1201 gaccggcccg aaaatgtagc cgaccagcga ggtggtgagc ggcacggtgc cgagccacag
     1261 gaagcccagc gcaccggcga agatcagcac cgaggcggcg gacagcggcg ccagcacgaa
     1321 gcccaggaat accagcgacc gcaggaggta gagcagcgcg agcaggttct tgcgcttgtg
     1381 gcggccgccc agggcgctcc agatgacggc gcccgcgata ttgaacagcc cgaccatgcc
     1441 gatcgcccaa ccgcccagct cggcgggcga gagattgatg ccgaacagcg agagcccgat
     1501 tcccttgtcc gagatatagg ccggcagatg tccgcccacg aaggcgacgt ggaagccgca
     1561 gacgaagtag ccgatcacca gcagcaggta gctcttctgc gcgaacgcct cggaaagcgc
     1621 ctcgcccgtc gtctggcggc caccgcccgc acgcctgccg gctgcgatct cccggctgtc
     1681 gttcagcccg accgacagca ggatcatcag cacggcaatg gcggtcagcg cccacatggt
     1741 gggccgccag tcgccgaaat agctctgcag caccgccgcc aggggcacga tgaagaactg
     1801 gccgaacgag ccgccggccg agcagatgcc gaccgccaga gcctgcttgg ccggcggcgc
     1861 cacccgcagg atgacgccca ggaccgggcc gaaggaggcc gccgacaagc cgacgcccac
     1921 caggatgttg cccaggatca gcatgaaacc gtcatgcatg gtggcggttg cgaccatgcc
     1981 cagcacgtag agcgcgccgc cgcccgcgat ggtcggcgcc gagccgaacc ggtcgcacaa
     2041 ccggcccgcg aagggtgcga cggcgcccat cagcagcatg gaaagcgcgg tgccgaagga
     2101 gaacagctcg cggccgacat gcagctcggc cgaaacgggc ttgagaaaga tgccgaagct
     2161 ctggcgcacg cccagaccga tggtgaggac gagaaaaccg caccagacag cggtccagta
     2221 ggaacgagag gtcatggcgg ccaacttgcg gccgggtggg cgccctgaca aggcgttcgc
     2281 cacagccttt ccatggaatt acttcatgcc gtcacaagcc acgatgtggc aaaacatgtc
     2341 catggcgcga ctgtggttcc gatgcggctt ggcagtattt ctgctggctt tggcaggcaa
     2401 tgccctcgcc atgccgaggc tcgaacggga acgctgcacg ttcaagccac cgcgcggcaa
     2461 tcgggtcgaa tgcttcgtgc tggtcgtgcc ggagaatcgg gagcagcccg aaggccgcga
     2521 ggtacggctg aaagtggccg tgctgaaagc caggcgcccg gcgaatgcgg agccgctgtt
     2581 ctatc
//
LOCUS       DASUGB010001065         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_258554, whole genome shotgun sequence.
ACCESSION   DASUGB010001065 DASUGB010000000
VERSION     DASUGB010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258554"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VFV87_20195"
     CDS             complement(<1..1732)
                     /locus_tag="VFV87_20195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5106157.1"
                     /translation="MPHELPIDAPPAGAPLPANPAQAADARLGAVNLLLSGLEAPALP
                     PESLLDSAELRAENKLIQVRLGVASSLFAALRAKHAPTAHHSLRVSLGCSAWGAMVGM
                     SEAERDELEVAALLHDIGKIGVPDEILLKPARLSAEEFLVVERHRDTGVEILRSCSAS
                     SDVLKIVQYAGAWFDGSRDGYDLRSEQLPLGARMVAIFDAFDAMTSDQVYRRAMSRER
                     ALAELFEFAGTQFDPRLVQEFCTYISADQMKLQAVVARRWLKQLTPEAGSNLWQLQTI
                     QASAAGNSLEGLFYRKLLEAMHDAVVFVDSTQRIVLWNRSAERLTGIAAASVEHKHWS
                     PTLISLRDERSQLLLDADCPVVHTIRSGVQTLRRLSITGRNGQQIEIDAHMVPVYGKS
                     GVAHGAALLMHDASSQVTLEQRVQTLHERATRDALTQVANRAEFDRLFGTCVESHLER
                     RLPCSLIICDLDHFKQVNDSFGHQAGDEVLVSFAALLRRHCRSGDLVARYGGEEFVLL
                     CPDCDNATATRRADEIRSELAELPMMALDGKPVTSSFGVTELQAGDTPETMLRRADRA
                     LYQAKENGRNT"
BASE COUNT          458 a          772 c          971 g          447 t
ORIGIN      
        1 ccgtattgcg gccgttttcc ttggcttgat agagggcccg gtcggcccgg cggagcatcg
       61 tctcgggcgt gtcgccggcc tgcagctcgg tgacgccgaa gctggaagtc accggcttgc
      121 cgtcgagcgc catcattggg agctcggcca gctcggagcg aatttcgtcg gcccggcgcg
      181 tggcggtggc gttgtcgcag tcggggcaga gcagaacgaa ctcttcgccg ccgtagcggg
      241 ccacgaggtc gccggaccgg caatggcggc gcaagagcgc cgcaaagctg accagcactt
      301 cgtcccccgc ctgatggccg aagctgtcgt tgacctgctt gaaatggtcc agatcgcaga
      361 tgatcaggct gcacggcagc cgccgctcca gatgcgattc gacgcatgtc ccaaacaggc
      421 ggtcgaactc ggcccggttg gcgacctgcg tcagggcgtc gcgcgtggcc cgctcgtgca
      481 gcgtttgcac gcgttgttcc agcgtcactt gcgacgaggc gtcgtgcatc aagagggccg
      541 ccccgtgggc gacgccgctt ttgccgtaga ccggcaccat gtgggcatcg atttcgatct
      601 gctggccgtt gcggccggtg atgctcaggc ggcggagcgt ctggacgccg gagcgaatgg
      661 tgtggaccac ggggcagtcg gcgtcgagca ggagctggct tcgctcgtcg cgcaggctga
      721 tgagcgtcgg cgaccaatgc ttgtgctcca cgcttgcagc ggcgatcccc gtcagccgct
      781 cggccgagcg gttccagagc acgattcgtt gcgtgctatc gacgaagaca acggcgtcgt
      841 gcatcgcctc cagcagcttg cgatagaaca ggccctccag cgagttgccg gcggcagacg
      901 cctgaatggt ttgcagttgc caaaggttgc tgccggcctc aggcgtgagc tgcttgagcc
      961 agcggcgagc gacgaccgcc tgcagcttca tctgatcggc actgatgtac gtgcagaatt
     1021 cctggaccag ccgcggatcg aactgggtgc cggcgaattc gaacagctcg gccagggccc
     1081 gctcgcgcga catggcccgg cgatacacct gatcgctggt catcgcgtca aaggcgtcga
     1141 aaatcgccac catccgcgcg cccagcggaa gctgctcgct ccgcagatcg tagccgtcgc
     1201 ggcttccgtc gaaccaggcg ccggcgtatt gcacgatctt cagcacatcg ctggaagccg
     1261 agcaggagcg gagaatctcg acgccggtgt cgcggtggcg ttcgacgacc aggaattcct
     1321 ccgcactcag gcgggcgggc ttgaggagga tctcgtcggg gacgccgatc ttgccgatgt
     1381 cgtgcagcaa cgcggcgact tccaattcgt cgcgctcggc ttcgctcatg ccgaccatcg
     1441 caccccaggc cgagcagccc agcgagaccc gcagcgagtg gtgggcggtg ggggcgtgct
     1501 tggcccgcag tgccgcgaag aggctgctgg cgacgcccag ccgcacctgg atgagcttgt
     1561 tctccgcccg caattcggcc gagtcgagca gcgattccgg cggcagcgca ggggcttcca
     1621 gcccgctgag cagcaggttg accgctccca ggcgggcgtc ggctgcttgc gcgggattgg
     1681 caggcagcgg cgctccggcg ggcggggcgt cgatcggtag ctcgtggggc atgagtcgct
     1741 ttcagcgacg tgcgacacgc gcgcggcagg gaccgggggc caatggaccg gacggtccgg
     1801 ctgcgcacgc gcgcagattg gccgaaattc cagagaaaac ctaggtcgca ggccagtgtc
     1861 cggcaaactt tagcgacgtc ggcgaagcgg cagaccgcag cggaccctgc cgcctttgcg
     1921 gcccctcacg gcggcgcgac tgaaatcact tgcgcctggc gtagcgggtg gtcggcgcag
     1981 acggattgtt ccgatcaaag cgccggggca gggcatccgg ccgcaccagg cagcacgatc
     2041 gggcacgcag cagtacgcat tgaagcggca gaccgttttc cgctcgatgc ctgcagcccc
     2101 gaacccagcg cgtttcgacg gtgaggtggt caagaatctc tggaaaaatt ccggtgcgga
     2161 gcaataacgg agggctatca atcgtctata cagtattact gtgttcttgt gtgatctatc
     2221 ttggcaaggt gggggatgtg ggtgatcgcg gctgtgggga ggctgcgagc agggcagggg
     2281 cgttattcag ggcgtgaatg aaatgaatag acgggggagc tgagattctg acgtaaggcg
     2341 ttgtggggaa atgggttgtg gcggtgatgg ccgcggaagg tttttgcgcg agtgaatgca
     2401 tggctggggg cagggttcgg ggagcaggag ccgggaggcg ggagccgcga ttgaaaagcg
     2461 caacagcgca acatcctcgg atgggggagg gcgtgtgatg gaaggcgtag ggcggctggg
     2521 agttgcggcg aatgttgcgc ggcggagttg cgcgaaaaaa gagcggcaag ttgcgctttg
     2581 cgtgggagcg gcgatccggc ggggagcggc aagatccggc ggggagcagg ataaagagca
     2641 ggagaaag
//
LOCUS       DASUGB010001066         5612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1506496, whole genome shotgun sequence.
ACCESSION   DASUGB010001066 DASUGB010000000
VERSION     DASUGB010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506496"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV87_20200"
     CDS             <1..239
                     /locus_tag="VFV87_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX5106158.1"
                     /translation="LRLPQPKFCDGQRLPAGYCNFLIANGVVIVPQFGDPADDEAIRT
                     LEPLFPGRDVRGCPSLNLIWGLGSFHCLSQQEPL"
     gene            complement(236..472)
                     /locus_tag="VFV87_20205"
     CDS             complement(236..472)
                     /locus_tag="VFV87_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106159.1"
                     /translation="MRTLQARHANLDWGATLKDIRSSWSEGERERRIRVAQQRQRRLL
                     RMLTPSRQSEAFDCHCDVEADEGGRSRRFVAVAG"
     gene            642..1538
                     /locus_tag="VFV87_20210"
     CDS             642..1538
                     /locus_tag="VFV87_20210"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5106160.1"
                     /translation="MRRIVIETVAAFWNDECPRLAAALAFYLVFALPALLGFTVLVTA
                     TIVDREAVAARLSAHLEEAMGPAQAQQVLQALAQARQPQQSWWAALAGAAVLVLSATG
                     ALQELQTALNRAWGVEPDPRQGRFAFLWKRLVSLGLLLAMAALLLVSLVASWALADLG
                     VWIDPYLPDWLSSRSIWIANGLLSLALVTILTAGVFKFMPDAEVAWTDVWVGAAVTAL
                     LFVAGKIGLSLYFTTAAPASAYGAAGSLALVLLWVYYSAQALLLGAEFTEVWARHRGR
                     IIGPEPGAKPAAENVRSASAGS"
     gene            complement(1558..2091)
                     /locus_tag="VFV87_20215"
     CDS             complement(1558..2091)
                     /locus_tag="VFV87_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106161.1"
                     /translation="MRRFFVSVAVAAALVSAAGAEQDPRQVEKFVAKGGEHWSAPAEI
                     SLGDSSVTGGKRLLVWPEGDKVFDVADKKALVSFHWMLLPGEAPFKRGQALRLSIHAP
                     AEPRFFTSTTVQPEAGAREGMLRVMLPLEKCPLGGETELVLYLGLKEPQSNLLEIKVD
                     LAPVAAAAPRPKQSKLR"
     gene            complement(2153..3769)
                     /locus_tag="VFV87_20220"
     CDS             complement(2153..3769)
                     /locus_tag="VFV87_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106162.1"
                     /translation="MVLAFRVAFCLALLTCLPLLVAQAAEGDWPQFRGPSATGVAEDS
                     KLPDEWSADQHIAWKVKVPGVAWSQPIVWGDKIFVTTAITENQPKPRTGGGGFGGGFG
                     GPGGPGGFGGRPGGFGGRPGGARPGGEQSTEEDRPREGDREPREGDRPREGAAQPRPG
                     AGRPGGFGAPGGFGGGFGGRPGGSGGGFGGGFGGRGASPPNAVYQWKLLCLDRNTGDV
                     VWEKLAKEAKPTIPIHGTNTYASETPITDGERIYVYFGMTGLYCYDLGGELLWSKELG
                     SHPMMMGWGAGSSPALDGDRLFVQCDNEEKSFLVALDKRSGDELWRAPRKEKSNWSTP
                     YVWKNKLRTELVTSGSTVTSYDPATGKVLWELGGNQGGARSTPVGDSERLYVGTGGGM
                     GGNGPLSAVHAGAKGDLSDGGEASDKHIAWSVPRSGPPMASPLLYEGCLYVLDQRGGI
                     LACYDAATGEQHYKQRIEGARGFTSSPWAYGGKVFCLDEAGTTFVLEPGSKYSVLGTN
                     KLDEMFWSSPALAHGQLLLRGVDHLYCIAE"
     gene            complement(3875..5485)
                     /locus_tag="VFV87_20225"
     CDS             complement(3875..5485)
                     /locus_tag="VFV87_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5106163.1"
                     /translation="MFVSRTFALLTLSALLAATCGCAGAGPWGPAGCGSCAAYRPESP
                     VIAPDARQVAQVAAQENSGLLVRAQSPGEYQPGSGNYLREPQPVSGGAPQVASNPAPA
                     FGAPPAGYAAPPAGYSAPPANFTPPPYQPAQPNVAYQPAPGGFGAPPLPPPPAEVMPP
                     ITPAYPGFMPPSNFPDVNATPTPLDVFVEETRTGKFMFGVAVNSDAGVTAQITIDERN
                     FDITKPPTSWDDFLNGTAWRGAGQGFRIEAQPGNQVQRYLVSFTDPYFLDTNISFSAS
                     GFYFDRNYFDWDESRYGGRLGWGYRLTPDLSLSTSVGGQNVQIFNPRVLGVPELDAVL
                     GKSDLFTGRVTLTHDTRDIPFMPTEGHLIELSYEQAFGSFDYPRGEVEFSQYFMLRER
                     PDGSGRHTLAYSTRFGISGADTPLYENYFAGGFSTLRGYAFRGASPIDMGVRVGGQLR
                     WLNTLEYFFPLTADDMIRGTVFCDFGTVERDIALNADNFRVAPGVGLRINVPALGPAP
                     LAFDFAFPLHSAPGDDEQMFSFFFGAMR"
BASE COUNT          945 a         1862 c         1799 g         1006 t
ORIGIN      
        1 ctctccgcct gccgcaaccc aagttctgcg acggccagcg ccttccggcg ggctattgca
       61 actttttgat cgccaacggc gtcgtcatcg tgccgcagtt tggcgaccca gccgacgacg
      121 aggccatccg cacgctcgag cccttgtttc ctggccgcga cgtccgcggc tgcccttcgc
      181 taaacctgat ttggggcctg ggttcgttcc attgcctctc tcagcaagag cccctttagc
      241 cggcgaccgc gacaaaacgt cgcgatcggc cgccttcgtc ggcttccacg tcgcagtggc
      301 aatcaaacgc ttcgctttgc ctggatggag tcagcatgcg cagcaatcgc cgctgtcgtt
      361 gctgggcaac gcgaatcctc cgttcccgtt cgccttccga ccagctcgag cgaatatcct
      421 ttagcgtcgc gccccagtcc aggttggcgt gccgtgcttg cagcgttctc atatccgtcc
      481 tcctgcgaag gtattagcgg atccggctcg acctcgatgg gcgggcactg ttgcaacctc
      541 cgtgccacgc accggcctgg tctttgctca gtcgattggg gaatccgcgc agattgtgcg
      601 cccttagaga aagacagacc ggacaaggga gacttggtca catgcggcgg atcgtgatcg
      661 agaccgtcgc cgccttttgg aacgacgaat gtccgcggct cgcggctgcc ctcgcgttct
      721 accttgtttt tgcgctgccg gcgcttctgg gtttcacggt cctcgttaca gctaccattg
      781 tcgatcgtga ggcggtcgcc gctcgccttt ccgcacacct ggaagaggcc atgggaccgg
      841 cgcaagccca gcaagtcctg caagctctcg cgcaggcccg ccagccgcag caaagctggt
      901 gggcggcgct ggcaggcgcg gcggttcttg tgctctcggc caccggagcg ctgcaggaac
      961 tccagaccgc tctgaaccgc gcctggggag ttgagcccga tccgcggcaa ggccggttcg
     1021 cctttctgtg gaagcggctg gtttcgctcg ggctgctgct ggcaatggcg gcgctgctgc
     1081 tcgtttcgct cgtggcgagt tgggccttgg cagatctggg cgtatggatc gacccttacc
     1141 tgccggactg gctctccagc cggtcgatct ggattgccaa cggtctgttg tcgctggcgc
     1201 tggtcacgat tcttacggcc ggcgtcttca aattcatgcc tgatgcggaa gtcgcctgga
     1261 cggacgtgtg ggtcggggct gcagtcacgg cgctgctgtt cgtagcaggc aagatcggcc
     1321 tgagtcttta ctttacgact gcggcgccgg ctagcgcgta tggcgctgcg ggctcgttgg
     1381 cgctggtgct gttgtgggtt tactattccg cgcaggcgct gcttttggga gcggaattta
     1441 ccgaggtctg ggcccggcat cgcggccgga tcatcggccc cgaacccggc gccaagccgg
     1501 ccgcggaaaa cgtgcgctcg gcgagcgccg gctcctgagc agagcggacg cgtcgcgtca
     1561 gcggagctta gattgcttcg gacggggcgc cgcagcagcc acgggcgcca aatccacttt
     1621 gatctccagc aagttgctct gaggctcctt cagtcccaag tacagcacca actcggtctc
     1681 gcctcctaac gggcatttct ccagcggcaa catcacgcgc agcattccct cgcgtgcgcc
     1741 tgcttccggc tgaacggtcg tggaggtgaa gaaccgcggc tcggcagggg cgtggatcga
     1801 gagccgcaat gcctggccac gtttgaacgg cgcttcgccc ggcaggagca tccaatgaaa
     1861 gctcaccagg gccttcttgt cggcgacgtc gaacactttg tcgccttccg gccaaacgag
     1921 cagccgcttt ccgcccgtca cgctcgaatc gcccagagag atttcagccg gtgcggacca
     1981 atgctcacca cccttggcga cgaatttctc gacctgtctg gggtcttgtt ctgccccagc
     2041 ggccgacacc agcgcagccg cgaccgccac cgatacgaag aaccgcctca tgatctctgc
     2101 tcctaagaca aggtggcgtt aggagcagca aatcccattc cgcgactgca ctttattctg
     2161 cgatgcagta cagatgatcg acgccccgca gcagaagctg accgtgagcc aaagcggggg
     2221 acgaccagaa catctcatcg agcttgttcg tgccgagcac gctgtacttc gatcccggct
     2281 caaggacgaa agtcgtgccg gcctcatcga ggcagaagac tttgccgcca tacgcccagg
     2341 gggacgatgt gaagccccgg gccccttcga tccgttgctt gtagtgctgc tcgccagtcg
     2401 cggcgtcgta gcaagcgagg atcccgccgc gctggtcgag cacgtacagg cagccttcat
     2461 acaacagcgg cgaagccatc ggcgggccgc ttcgcggcac actccaggcg atgtgcttgt
     2521 cggacgcttc gccgccgtcc gagagatcgc ctttcgcgcc ggcatgaacg gccgacaatg
     2581 gaccattgcc acccatgccg ccaccggttc ccacgtacag ccgctccgaa tcaccgacgg
     2641 gcgtcgagcg agcgcccccc tgattgccgc ccagttccca cagcaccttg ccggtggccg
     2701 gatcgtagga cgtcaccgtg ctgccggacg tgaccagttc cgtccgcagc ttgttcttcc
     2761 agacgtaggg cgtggaccag ttcgatttct ccttgcgcgg ggcccgccac agctcgtcgc
     2821 cgcttctctt gtcgagcgcg acgaggaacg acttctcctc gttgtcgcac tgcacaaaca
     2881 gccgatcgcc gtccaatgcg ggcgaactgc cggcgcccca gcccatcatc atcggatgcg
     2941 agcccagctc cttgctccac agcagttcgc cgcccagatc gtagcaatac aggcccgtca
     3001 tgccgaaata aacatagatc cgctcgccat cggtgatcgg cgtttcggag gcgtacgtgt
     3061 tggtgccatg gatcggaatc gtcggcttcg cttcttttgc cagcttctcc cacaccacgt
     3121 cgcctgtgtt ccgatcgagg cagagcaact tccactgata gacggcattg ggcggcgaag
     3181 ctccgcgccc gccaaatcct cccccgaagc cgcctccaga tccgcccggc cggccgccaa
     3241 agccgccgcc gaacccgccc ggtgcgccaa acccgccagg acgccccgcg cccggccgcg
     3301 gctgagccgc gccttcgcgc ggtcgatccc cttcacgcgg ctcgcggtca ccctctcgcg
     3361 gccgatcctc ttcggtggat tgctcgccgc cgggccgcgc acctcccggc ctgccgccga
     3421 atccgcccgg cctgccgccg aatccgccag gacctccggg gcctccgaat cctccgccaa
     3481 aaccgccccc gcccgtgcgc ggcttaggct gattctcggt gatcgcggtg gtcacgaaga
     3541 tcttgtcgcc ccacacgatt ggctgcgacc aggccacgcc cggcaccttc accttccacg
     3601 caatgtgctg gtccgcgctc cactcatccg gcagcttcga gtcctccgcc acacccgtcg
     3661 cactcggtcc ccggaattgc ggccagtcgc cctcggcggc ttgggcgaca agcaaaggaa
     3721 ggcaggtcag caaagccagg caaaacgcga cacgaaaagc gagaaccatg attactccct
     3781 gggagacggg ctaaaagcca tcggcccggt ggaaaacgga ttatcgatgc gcccgaccag
     3841 ttaaaccgat ttcaccgccg tttgttgcgg gaaatcaccg catcgcgccg aagaagaagc
     3901 tgaacatctg ctcgtcgtcg ccaggcgccg agtggagcgg gaaggcaaag tcgaacgcca
     3961 gcggcgccgg gccgagagcc ggcacgttga ttctcaggcc cacgccgggc gctacgcgga
     4021 agttgtcggc gttgagcgcg atgtcgcgct ccacggtgcc aaagtcgcag aaaaccgtgc
     4081 cgcggatcat gtcgtcggcg gtgagcggga agaagtactc cagcgtgttc agccagcgga
     4141 gctggccgcc gacgcggacg cccatgtcga tcggcgatgc tccgcggaag gcataaccgc
     4201 gcagcgtcga gaagccgccc gcgaagtaat tctcgtacag cggcgtatcc gcgcccgaaa
     4261 tgccgaaccg cgtggaatag gccagcgtgt gccgccccga gccgtcgggc cgctcgcgga
     4321 gcatgaagta ctgcgagaac tcgacttcgc cgcgggggta atcgaacgag ccgaacgcct
     4381 gctcgtacga cagctcgatg agatgccctt cggtcggcat gaaaggaatg tcgcgcgtgt
     4441 cgtgcgtgag cgtgacccgg ccggtgaata agtcgctctt gcccagcacg gcatcgagtt
     4501 ccgggacgcc gaggacgcgg ggattgaaga tctgcacgtt ctggccgcct acggacgtgc
     4561 tgagcgacag gtcgggcgtc aggcgatagc cccagcccaa gcggccgccg tagcgcgatt
     4621 catcccagtc gaaatagttg cggtcgaaat aaaagccgct ggcgctaaag ctgatattcg
     4681 tgtccaggaa gtacggatcg gtgaaactga cgagatagcg ctgcacctgg ttgcccggct
     4741 gggcttcgat gcggaagccc tggccggccc cgcgccaggc ggtgccgttg aggaagtcgt
     4801 cccagcttgt cggcggcttg gtgatgtcga agttgcgttc gtcgatcgtg atctgggccg
     4861 tcacgccggc atcggaattc accgccacgc cgaacatgaa cttgccggtg cgcgtctctt
     4921 cgacgaacac gtccagcgga gttggcgtgg cgttgacgtc cgggaagttc gacggcggca
     4981 taaagccggg ataggccggc gtgattggcg gcatcacctc ggcgggtggc ggaggaagcg
     5041 gcggagcgcc gaacccgccc ggtgcgggct gataggccac gttcggctga gcgggctgat
     5101 aggggggagg cgtgaagttg gccggcgggg cggaatagcc cgcgggagga gcggcgtaac
     5161 ccgcgggagg agcgccgaag gccggcgcgg gattgctcgc cacctgggga gcgccgccgg
     5221 agaccggctg gggctcgcgg aggtagttgc ccgatccggg ttgatactcg cccggcgact
     5281 gggcgcggac aagcagcccg ctgttctctt gagccgccac ttgagcgact tgccgggcgt
     5341 ccggcgcgat cactggcgac tcgggccggt aggccgcgca gcttccgcag cctgccggtc
     5401 cccatggccc agcgcccgcg cagccgcaag ttgcggcgag cagcgcgctg agggtcagca
     5461 gggcaaaggt gcgggagacg aacaaaacgc gattctcgtg cggggttgcg ggaaatcggt
     5521 cgcttaccgg tacgggctcc agcgggtctg gttcggggaa tgtgagccgg ggtcttggcc
     5581 gcgaaccgtc ggcttcgttt gctccgcgat cg
//
LOCUS       DASUGB010001067         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1091104, whole genome shotgun sequence.
ACCESSION   DASUGB010001067 DASUGB010000000
VERSION     DASUGB010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091104"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2451
                     /locus_tag="VFV87_20230"
     CDS             <1..2451
                     /locus_tag="VFV87_20230"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5106164.1"
                     /translation="TILPFRNRDLTMTAGRSSVMAWWCALTIFVSAFLLFQVQPVISK
                     TILPWFGGSPAVWTTCVLFFQLMLLAGYAYADWLVQRVPAHRQGLIHAAVVVLAVLTL
                     PITPGDFWKPLDGSWPAPRILLLLAAKVGAPFFLASTTGPLVQAWFSQLYPGRSPYRL
                     YALSNVGSLAALLSYPFLFEMLLDVRTQGNLWSLGFVVFAVLIGYLSITMWRVSSEKI
                     PEAIEPAEVGGLPVPRPAEKLAKPEPKPTDEAPPTWWLRAAWLGLAALGTTSLLSITN
                     HLSQDIAVVPFMWVIPLSLYLLSFIICFDNERWYQRKLWGTLTILMIAWLAATLNYES
                     VDEFAEWVQKPVTPTIENGETKSVIPLTLSDINDATVFPAVGWLGDASNKALNAVFSW
                     TGMSARGWKADWNLHTTTEDFAEHVIVISAAWILTLFLICMVCHGELVKSKPQPKYLT
                     NFYLFISAGGAVGGLFVALLCPLLFKLHFELALSIICGYIVGAIALANDGRNSWLKGR
                     EILQWSAAFLIVGGVLLVSKAMVEGIEDDTVAISRNFYGTVTVKTLKDDEDPSGDGLA
                     LYNGRIWHGFQYSDPARAMEPTTYYVDGTGAALSVHSHPRADEPLRVAVIGLGTGTMA
                     VHGRKGDVFRFYDIDPKVVKVAQTYFTYLKDSDAKTEVVLGDARISMERELRTGGSQN
                     YDVIVLDAFSGDAIPAHLLTDEAFSIYHQHLRRDMVDGQEVPMGVVVVHISNRYLDLE
                     PVVAAIARKHGYQTRLIHTEEGFAASDTASDWVLVSQSEEFLNNERVSALGQPLEPKF
                     ELLWTDQRTSLFPILQRE"
     gene            complement(2455..>2837)
                     /locus_tag="VFV87_20235"
     CDS             complement(2455..>2837)
                     /locus_tag="VFV87_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEX5106165.1"
                     /translation="AQRLVRVLCQQCRVAYDISEAPDTFAEIKPLLEPGLGGFIYGPS
                     GCEACRQQGYSGRIGVFEVMTFNRQLRQMILQQKAAEELQQQAFAGGMIEFRRGSMLK
                     VAQGLTSTEEVLRELPAEYLGLEG"
BASE COUNT          480 a          921 c          876 g          560 t
ORIGIN      
        1 accatccttc cattccgcaa ccgggacctg acgatgaccg ccggccgctc ttccgtgatg
       61 gcctggtggt gcgcgttgac gatcttcgtc agcgcgttcc tgctcttcca ggtccagccg
      121 gtcatcagca agacgatcct cccgtggttt ggcggcagcc cggcagtgtg gacgacgtgc
      181 gtgctgttct tccagctcat gctgctggcc ggctatgcct atgccgattg gctggtgcag
      241 cgcgtccccg cccaccggca ggggctgatt cacgcggcgg tcgtcgtcct ggcggttctc
      301 acgctcccga tcacccccgg cgacttctgg aagccgctcg atggctcgtg gccggcgccg
      361 cgcattctct tgctcctggc tgcgaaggtt ggcgcgccgt tttttctggc ctcgacgacc
      421 ggtccgctgg tgcaggcctg gttcagccag ctttatcccg gccgctcgcc ctatcgcttg
      481 tatgccttgt ccaatgtcgg ctcactcgcc gcgctgctca gctatccatt tctgttcgag
      541 atgctgctgg acgtgcggac gcagggcaat ctctggtcgc ttgggttcgt cgtcttcgcc
      601 gtcctgatcg gctacctgtc gatcacgatg tggcgcgtct ccagcgagaa gatccccgaa
      661 gccatcgaac cggcagaagt cggcggcctg ccggtcccgc ggccggccga aaagctcgcc
      721 aaacccgaac ccaagccaac cgacgaggcc ccgcccacct ggtggctgcg ggccgcgtgg
      781 ctggggctgg cggcgctcgg aacgacctcg ctgttgtcga ttacgaacca ccttagccag
      841 gacatcgcgg tcgtcccctt catgtgggtc attccgctga gcctgtacct gctgtcgttc
      901 atcatctgtt tcgacaacga gcgctggtat cagcggaagc tgtgggggac gctgacgatc
      961 ctgatgatcg cctggctggc cgccacgctg aattacgaga gcgtcgatga attcgccgaa
     1021 tgggtccaaa agccggtcac gccgactatc gagaatggcg agaccaagtc agtcattccg
     1081 ctgacgctga gcgacatcaa cgacgcgacg gtattccccg ccgtcggctg gttgggcgat
     1141 gcgtcgaaca aagctctcaa cgcagtgttt tcgtggaccg gcatgtccgc ccgcggctgg
     1201 aaggccgatt ggaatctgca cacaaccaca gaggactttg ccgagcacgt gatcgtgatc
     1261 tcggcggcct ggattcttac gctctttctg atctgcatgg tctgccacgg ggaactggtc
     1321 aaatcgaaac cgcagcccaa gtacctgaca aacttctacc tgttcatttc cgccggcggc
     1381 gcggttggcg ggctgtttgt ggcgctgctc tgtccgcttc tgttcaagct gcacttcgag
     1441 ttggcgctat cgatcatctg cggctatatc gtgggcgcca ttgccctggc caacgacggc
     1501 cgcaactcgt ggctcaaggg gcgcgagatt ctgcagtgga gcgcggcgtt tttgatcgtc
     1561 ggcggtgtgc tgctcgtctc gaaggcgatg gttgagggga tcgaggacga tacggtcgcc
     1621 atctcccgca atttctatgg cacggtgacg gtcaagacgc tcaaggacga cgaagacccg
     1681 tcgggcgacg ggctggctct gtacaacggc cgcatctggc acggcttcca gtacagcgac
     1741 ccggcgcggg ccatggagcc gacgacgtac tatgttgacg gtaccggcgc tgcactctcg
     1801 gtccacagcc atccgcgggc cgatgaaccc ttgcgggtgg ccgtgattgg cctgggaacc
     1861 ggaacgatgg ccgtccacgg acgcaagggg gacgtattcc gtttttacga tatcgatccc
     1921 aaggtggtga aggtcgccca gacctatttc acctatctga aagacagcga cgccaagacc
     1981 gaagtcgtgc tgggcgacgc ccggatcagc atggagcgcg agctgcggac gggcggttcg
     2041 cagaactacg acgtgatcgt tctggacgcc ttcagcggcg acgcgatccc ggcccacttg
     2101 ctgacggacg aggcgttctc gatctatcac cagcacctgc gccgcgacat ggtcgatggc
     2161 caagaagtgc cgatgggcgt cgtggtcgtc cacatcagca accgctacct cgacctggaa
     2221 cctgtcgtgg ccgccatcgc caggaagcac ggctaccaga cgcggctcat tcacaccgaa
     2281 gaaggctttg ccgcgtccga caccgcctcc gactgggtgc tcgtgagcca gagcgaggaa
     2341 ttcctcaaca acgagcgggt cagcgcactg ggccagcccc ttgagcccaa gtttgaactc
     2401 ctctggaccg accagcggac aagcctgttc ccgatcctgc agcgggagtg aacgctaccc
     2461 ctccagcccc aaatactccg ccggcagctc gcgcaggact tcttcggtgc tggtcaggcc
     2521 ttgggcgact ttgagcatcg agccgcggcg gaattcgatc atcccgcccg cgaaggcctg
     2581 ctgctggagc tcttcggccg ccttctgctg caggatcatc tgccggagct gacggttgaa
     2641 ggtcatcact tcgaagacgc cgatccgccc ggaatagccc tgctggcggc aggcctcgca
     2701 gccgctgggg ccgtaaataa acccgccaag ccccggctcc aggagcggct tgatctcggc
     2761 gaacgtgtcc ggggcctcgc tgatgtcgta ggccacccga cattgctggc agaggaccct
     2821 caccagccgc tgggcca
//
LOCUS       DASUGB010001068         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2064268, whole genome shotgun sequence.
ACCESSION   DASUGB010001068 DASUGB010000000
VERSION     DASUGB010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064268"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFV87_20240"
     CDS             complement(<1..258)
                     /locus_tag="VFV87_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106166.1"
                     /translation="MTISTAELLAPQTIRKAISQLELPGTSLQTLFGWGLGGTNLARV
                     AGRNFSYDIFNHARTVATGRVPKQSLARTKAQSASHVAATFP"
     gene            complement(404..1183)
                     /locus_tag="VFV87_20245"
     CDS             complement(404..1183)
                     /locus_tag="VFV87_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106167.1"
                     /translation="MSHFTTGNAPGFQTPIGADEKQVTWSGRRGQDLVATRQITLTGG
                     ATDGGNTPNTTLRGGILLASVDATGLAHTYSPDANDGRQIAIGILEQSQDMLVAGSAT
                     DRFTQMLVHGLVKEGELINLDPRARQQLGSRFTLDRERSPQAGVLMHPRGIYRKSANY
                     TVTADDNGLLFLATAAVTFTLPTKENGLAFRFAQTADANLVIAGSSDLIALNNAAASS
                     VTFSTASEKIGSQVLVECLYTAAATLKWLVTNLGGTTPTVA"
     gene            complement(1359..1958)
                     /locus_tag="VFV87_20250"
     CDS             complement(1359..1958)
                     /locus_tag="VFV87_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106168.1"
                     /translation="MSAQSARGAEPVEDRQEEVIEEGPQSGEEARSHAERGNEGTPVE
                     QEDTEVSEEDTVLAEAAPQSGEDGRSHAERGNEEPSELAAEARQRAAGRVREAARVPP
                     GMRERLAALVESGGQIGADGVALVSIDEALCAVAEGLPNSVRLRGEELARPGHPGGEA
                     FFTSAGGEVLSDERAEELARGQLARSGLLRGQRVRVAQD"
     gene            complement(2406..2777)
                     /locus_tag="VFV87_20255"
     CDS             complement(2406..2777)
                     /locus_tag="VFV87_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106169.1"
                     /translation="MKQNPSACFWASCYRLYELWSPLPNRLAAEESTGRRGLRYAVAA
                     WYCGVYLLAAVGIWRLRWSLLKPPWVWGVLLCVVFTGAHTFYWSNLRMRAPLMPFVAL
                     VAGAALLPGRNRPRRNAEERG"
BASE COUNT          465 a         1017 c          893 g          515 t
ORIGIN      
        1 cgggaacgtc gccgcgacgt ggctggcgct ctgggccttc gtccgcgcca gcgattgctt
       61 gggcacgcgg cccgtggcca cggtgcgggc gtggttgaag atgtcgtacg aaaagttccg
      121 cccggccacg cgggcgaggt tcgtcccgcc caggccccag ccgaacagcg tctgcaggct
      181 ggttcccggc agctcgagct ggctgatggc cttgcggatg gtctggggag cgaggagttc
      241 tgcggtggaa atggtcatgg gtttctccga tggaagttgg ttgaatgagt tgaatgaaac
      301 ggttggaaaa tggggggacg ggggacaggg aacgggggca gagggcgttg gagcggaggg
      361 tcgtccctct gcccctgccc cctgttccct gttcccttgg ccgctaggcg accgtcggcg
      421 tcgttccgcc gaggttcgtc accagccact tcagcgtggc ggcggcggtg tagaggcact
      481 cgaccagcac ctggctgccg atcttttcgc tggccgtgct gaacgtgacg ctgctcgccg
      541 cggcgttgtt cagcgcgatg aggtcgctcg aaccggcgat caccaggttc gcgtcggccg
      601 tctgggcgaa gcgaaacgcc aggccgttct ccttcgtcgg cagcgtgaac gtgaccgccg
      661 cggtggccag gaacagcagc ccgttgtcgt ccgccgtgac ggtgtagttc gcactcttgc
      721 gatagatgcc ccgcgggtgc atcagcacgc cggcttgcgg cgagcgctcg cggtcgagcg
      781 tgaagcggct gccgagctgc tggcgggctc gcggatcgag gttgatcagc tcgccttcct
      841 tgaccaggcc gtggacgagc atttgcgtga agcggtcggt cgccgagccg gcgacgagca
      901 tgtcctgcga ctgctcgagg atgccgatgg cgatctgccg cccgtcgttg gcgtcgggcg
      961 agtaggtgtg ggccaggccg gtcgcatcga cgctggccag caagatgccg ccgcggagcg
     1021 tcgtattcgg cgtgttgccg ccgtcggtcg cgccgccggt gagcgtgatc tgccgcgtcg
     1081 cgaccaggtc ctgcccgcgc cggccgctcc aggtgacctg cttctcgtcg gcgccgatcg
     1141 gcgtctgaaa acccggcgca ttgccggtgg tgaagtgaga catcgcgttc tccttgctgt
     1201 gaaagtgatg gaaatggaaa atcaatcgcc tcattagccg ggagcaaact tgctcccggc
     1261 agtggggcag gcatcttgcc tgccgaaaaa ccggctcatg gcaggctgga agcctgcccc
     1321 acgttctccg ggaccaggtt tggtcccggc taagaagttc aatcctgcgc cacccggacg
     1381 cgctgcccgc gcagcaatcc gctgcgggcg agctgcccgc gggccagctc ctccgcgcgc
     1441 tcgtcgctca gcacctcgcc gcccgcggac gtgaagaacg cctcgccccc cggatgcccc
     1501 ggccgcgcga gctcttcgcc gcggaggcgc accgaattcg gcagcccctc ggctaccgcg
     1561 cacagcgcct cgtcgatcga gaccagcgcc acgccatccg cgccgatctg cccaccgctc
     1621 tccacgagag ccgccaagcg ctcccgcatc cccggcggca ccctcgccgc ctcccgcacc
     1681 cgccccgccg cccgctggcg cgcttcggca gcaagttccg acggctcctc gttcccacgc
     1741 tctgcgtggg aacgcccgtc ctcgccgctc tgcggcgctg cctccgcgag aacggtgtcc
     1801 tcctcgctaa cctccgtgtc ttcctgctcc accggcgtgc cctcgttccc acgctccgcg
     1861 tgggaacgcg cttcctcgcc gctctgcggc ccttcctcaa taacttcctc ctgccgatcc
     1921 tcaaccggct ccgcaccgcg cgcactctga gcgctcatca ctggacctcc tcgtgaaaga
     1981 aagaatcgaa ttcgtgccgc cggaaagcac cgggcggcag cggtcagtcg cgtcgatttc
     2041 tcatccgcgt ctgacgggcg tgggttttac gaaccgcgtc cctgcgctcc aaacgaattc
     2101 ttggaaaatt gtcgggtgaa caccaactta cgtccgttca ctatatcgca actcccggcc
     2161 tgctagcaac ttcggctgag gcgcgtcgtc gtaagttttt ttttgcgcgc caaacgaaaa
     2221 ggacacgtct ggtccattcc ggaagctccc acgcgctagt gtacaaactg attttgcccc
     2281 tcgtacacca acccgcagcg tcagcgaggg acaccaaccc gcagcgtcag cgagggagtc
     2341 cagcggcgga ttcacaaccg cggatgtcgc gaaaagacgc ggattccgcc ggcttctatc
     2401 cgcgttcatc cgcgttcctc cgcgttcctc cgcggccgat ttctgccagg cagcagcgcc
     2461 gctccggcca ccaacgccac aaacggcatc aagggcgccc gcatccgcag gttcgaccag
     2521 tagaacgtat gcgcgccggt aaagacgacg cacagcagca cgccccacac ccaaggcggc
     2581 ttcagcaaac tccaccgcag ccgccagatt cccacggccg ccagcaaata cacgccgcag
     2641 taccaggccg ccaccgcata ccgcagcccc ctccgccccg tcgattcctc cgccgctaac
     2701 cgattcggca gcggcgacca gagctcgtac aggcgatagc aacttgccca aaagcacgcg
     2761 ctcgggttct gtttcatgtg tgctgttgca atgctgtaaa agcaatcgtc tcgcgccact
     2821 tctgtaggca aatctgacac ctcccttgcg accaaatgga tttcggcgtc atattcgcct
     2881 gcgccccacg
//
LOCUS       DASUGB010001069         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_813437, whole genome shotgun sequence.
ACCESSION   DASUGB010001069 DASUGB010000000
VERSION     DASUGB010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813437"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFV87_20260"
     CDS             <1..264
                     /locus_tag="VFV87_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106170.1"
                     /translation="SRLEAIEREYAAAVGGRSEESGVGSQKPSSDDVRRIISDLDDQG
                     RWISTYAGERLVGQPKFKLGYQYLDSGVFSRNLNAISAFLEKE"
     gene            415..496
                     /locus_tag="VFV87_20265"
     tRNA            415..496
                     /locus_tag="VFV87_20265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Ser,seq:gga)
     gene            complement(710..1867)
                     /locus_tag="VFV87_20270"
     CDS             complement(710..1867)
                     /locus_tag="VFV87_20270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5106171.1"
                     /translation="MSRKRRGNGEGSLYQRADGTWCATISVGYDSQGRRKRRTIFGES
                     KQDVQNKLAKLANEVAHQRDIEPQRIKLGEYLDRWLQDAAKPRVRETTHANYAGVIKN
                     HIQPHLGGVPLAKLTAFQIHGLYSCLEQAGKSAETIRLVHAVLHRALKQAVRWRLIPF
                     NMAADVDRPKAVKTDISPLTADQVNALLKAARGNRQEALYVLAVTAGMRLGELLGLQW
                     SDVDLKGKAVMVQHSLQELNGQLKLAEPKTSRGRRRIDLPQMAIDALVRHRARLLKEG
                     LAGVAWVFPNASGGPWRRTHFHFNEYKPLLKRAGLPDIRFHDLRHTSATLLLSQGVHP
                     KVVQERLGHSQISVTLDTYSHVLPTMQLEAAGKFDQLLQTTSKAGRKTALS"
     gene            complement(1868..2929)
                     /locus_tag="VFV87_20275"
     CDS             complement(1868..2929)
                     /locus_tag="VFV87_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106172.1"
                     /translation="MLTSTVVSFRWQPPAGGHRWVQAHPVTDNWAVTREKVEVLAPFG
                     FPMFWETYGKPEAAYESLETASGLFRELAALDAGPEAILAFANKYGPLTPGRLFVLVG
                     NHGKFPRPPLPRSHTQEPVRPLNTGGRGDEPEWTAKWLTSGAVAGDSLAFWHQHIRAM
                     RALVGLWDALQGRDRAAIEEVARFTWQGKKRAVGLVDERGNSLGESIRFDQASSASLT
                     LQRAAEHALIRALAGFTSDGASIRLFPPDSRHQRRLAIVPRSLIDAIWLQFALAVLEN
                     KTYRECEVCGKPFEISPQVARTSRTLCSAACKARAHRQRRDQALKLANQGVAPRQIAK
                     RVGSQLSTVQNWLSEAKEK"
     gene            3064..3282
                     /locus_tag="VFV87_20280"
     CDS             3064..3282
                     /locus_tag="VFV87_20280"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5106173.1"
                     /translation="MPRTNDDANPDQPDNGLQPVNKVARFLSLSRSKVYALMEAGELP
                     YVKLGKSRRVRWSDVLELVARNTVVRGS"
BASE COUNT          592 a         1042 c         1071 g          617 t
ORIGIN      
        1 tcgcggctgg aggcgatcga gcgggagtat gctgcggcgg tgggcggcag gagcgaggag
       61 tccggggtcg ggagtcagaa accctcctcc gacgacgtcc ggcggatcat ttccgatctc
      121 gatgaccagg gtcgctggat cagcacctat gccggcgagc gcctcgtcgg ccagccgaag
      181 ttcaagcttg ggtatcaata cctcgacagc ggcgtcttca gccggaatct taatgcaatc
      241 agcgccttcc tggagaagga gtaaaggccg atgcatcggg accagatgca acttggtgcg
      301 ccgaacctga cggtgacact tggcccacac tcccgcgaaa tcgaccaagt ctgtacgatg
      361 accgaggaga aatcgtggat ttcgtttcat tcacgcgatg tcgaccgatt tcttggaggg
      421 taagcgaatg gttagcggcc acactggaaa tgtggtgccc cgcaaggggt tgcgggttcg
      481 agccccgtgc cctccgctct tctaaactcc gcccgcgtct agagttgcgc gggctttctt
      541 ttgcgcggag cagcgccaga ctcgggggat ttcgcggcca ctgtagccac aatgtagcca
      601 atcttgggat gtcggcgcaa cacacgcgga tagacggcca aaaacgatgg cggactcgcg
      661 ttttgcaacc aaagttggac gactacggac ggagtgaaac gtcctgattc taggataacg
      721 cggtcttcct gccggcctta ctcgtcgtct gcagcaactg atcaaacttc ccggccgcct
      781 cgagctgcat cgtcggcaga acgtgcgagt acgtgtccaa cgtcacgctg atctgagaat
      841 ggccgagccg ctcctggacg accttgggat gaaccccttg cgacaagagg agcgtggccg
      901 acgtatgccg caggtcgtgg aatcggatgt ccggcaggcc tgcacgcttc agcagcggct
      961 tgtactcgtt gaaatggaag tgcgtgcggc gccacgggcc gcctgaggcg ttggggaaca
     1021 cccaggcgac gccagccagg ccttccttta gcaatcgagc ccggtggcga acgagggcgt
     1081 cgatcgccat ttgcggcagg tcgatccggc gccggccccg cgacgtcttc ggttcagcga
     1141 gcttcaattg gccattgagt tcctgaaggg agtgctggac catcacggcc ttccccttca
     1201 gatcgacgtc cgaccactgc aggcccaaga gctcgccgag acgcatgccg gccgtgacgg
     1261 ccagtacgta gagcgcctcc tggcggttgc cgcgggcggc cttcagcaat gcgttgacct
     1321 ggtcggccgt caacggcgag atgtctgtct tgacggcctt gggacgatcc acgtcggcgg
     1381 ccatgttgaa ggggatcagc cgccagcgga ccgcctgttt tagagctcgg tgcaagacgg
     1441 cgtgaaccag ccggatggtc tcagcgcttt ttcccgcctg ctccaggcac gagtacagcc
     1501 cgtggatctg gaaggccgtg agtttggcca ggggcacgcc ccccaggtgt ggctggatgt
     1561 ggttcttgat gacccctgcg tagttcgcgt gggtcgtctc gcggacccgt ggcttggccg
     1621 catcctggag ccagcgatcg aggtactcgc ctaacttgat ccgctggggc tcgatgtcgc
     1681 gttggtgggc cacttcattg gcgagcttcg ccaacttgtt ctgcacatcc tgcttcgact
     1741 cgccgaagat cgtccgacgt ttccgccttc cctggctgtc gtagccgacg ctgatcgtgg
     1801 cgcaccacgt gccgtcggcc cgctggtaga gcgacccttc accgttcccg cggcgcttgc
     1861 gtgacattca tttctccttg gcctcggaaa gccagttctg aaccgtggaa agctgggagc
     1921 cgacgcgctt ggcaatctgc cggggagcca ccccctgatt cgccaatttc aatgcctggt
     1981 cccgccgctg ccggtgagcg cgggccttgc aggcggcact gcacagcgtc cggctggtcc
     2041 gcgcgacctg gggggagatc tcaaacggct tgccgcagac ctcacactcg cgataggtct
     2101 tgttttccag gaccgccagg gcgaattgca gccagatcgc gtcgatcaac gaccgcggaa
     2161 cgatggccag ccgccgctgg tgacgcgaat cggggggaaa caggcggatc gacgccccgt
     2221 ccgatgtgaa ccccgccaag gcccggatca acgcgtgttc ggctgcccgc tggagtgtca
     2281 acgacgcgct gctggcctga tcgaagcgga tggactcccc cagggagttg ccccgttcat
     2341 ccaccagtcc gactgcgcgt ttcttccctt gccaggtgaa ccgggcgact tcttcgatcg
     2401 ccgcgcgatc gcgcccctgg agggcgtccc acaagccgac gagggcccgc attgcccgga
     2461 tgtgctggtg ccagaatgcg agactgtcac ccgctacggc accgctcgtt aaccacttcg
     2521 cggtccattc cggctcgtcg cctcgccctc cggtgttgag cggccggact ggctcttgcg
     2581 tatgtgaccg ggggagaggc ggccgaggga atttcccgtg gttccccacc agcacgaaca
     2641 atcgccctgg cgtcagaggg ccatatttat tggcgaacgc caggatggcc tcgggacccg
     2701 cgtcgagcgc cgccagttcg cggaacaggc cgctggccgt ttccagcgat tcgtaggctg
     2761 cctcgggctt cccgtaggtc tcccagaaca tgggaaaacc gaacggggcg agcacctcga
     2821 ccttctcgcg ggtgaccgcc cagttgtcgg tgacgggatg cgcctggacc cagcggtgac
     2881 cgccggcggg cggctgccag cggaaggaaa cgaccgtgga cgtgagcatg gcgaaatctc
     2941 caaagtaacc gaaactgtaa ccgtaagtag acccggtacg gttgtgagtg tcaacggcag
     3001 gtgttaccgt aacaatcata gaaacggttt ccggaccacc ctacccacga cggagcgcca
     3061 cgcatgccca ggacgaatga cgacgccaac cccgaccagc cggacaacgg actgcagccc
     3121 gtgaacaagg tggcccgctt cctcagcctg agccggtcca aggtgtatgc cctgatggag
     3181 gccggcgaat tgccctatgt gaagctcggc aagagccgcc gcgtgcggtg gtcggatgtg
     3241 ctcgagctcg tggcccgtaa caccgtggtg cgcggctcgt agggtcggca cctatggccg
     3301 gcggctgcaa gttgcgcccg at
//
LOCUS       DASUGB010001070         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_674508, whole genome shotgun sequence.
ACCESSION   DASUGB010001070 DASUGB010000000
VERSION     DASUGB010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674508"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFV87_20285"
     CDS             <1..247
                     /locus_tag="VFV87_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106174.1"
                     /translation="SRWSTKRSTEWQPLRPTLVVEVQYDHFTGGRFRHGTKLLRWRPD
                     KSPDMCTRQQVNQRGAKLKLLAKPVRARRKTAARSKG"
     gene            422..769
                     /locus_tag="VFV87_20290"
     CDS             422..769
                     /locus_tag="VFV87_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106175.1"
                     /translation="MDSTSKRAARDEIADMVVDWLTRSSKLSELATADLETIEDEVVE
                     NIDAITRSAISKLLDQQRRQSETPQACPQCGGCLSAKPAQRRTMQSRRGNIHFSCDVF
                     HCPACRRDFFPSV"
     gene            810..>2090
                     /locus_tag="VFV87_20295"
     CDS             810..>2090
                     /locus_tag="VFV87_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106176.1"
                     /translation="MLKKVARAAVRHGSYEKASVNLQDMADLKVSAKQVQRVAIRLGE
                     ERLAEQQQRVARYQAASLPERQGQPPEAPRNNWSGRAAAIQCDGGRAQIRDELWDAEK
                     PPGKRHRWWRETQSGVLQTYLSQPAQQDPHPEVPAELMDPLWAVPKFNEIHCAHAAAG
                     SPDSPAETGDSDASARAGDACASDDPSERSEAARRKGKGAAKNKSKKKPPPWSGGSPL
                     VKTVVATRRGYDHLGLLLAAEAFMRGFNLATAKAFLGDGLKVNWTIWSRHFSHYTPIV
                     DLMHALSYVYAAAVACSPTIEDGWSMYLRWLAWVWAGNVSLVLEAMREIAAGRTDRVE
                     SLDRAITYLSNNASRMKYAEYRQAGLPITTALIESTQKQINGRIKGTEKFWCDERLEP
                     LLQLLQDDLSDTYDSQQFWHRRRQRFTGFRNRRAK"
BASE COUNT          425 a          613 c          683 g          369 t
ORIGIN      
        1 cagccgctgg agcacgaagc gctcgaccga gtggcagccg ctgcggccga cgctggtggt
       61 cgaagtgcaa tacgaccact tcaccggcgg ccgcttccgc cacggcacga agctgctccg
      121 ctggcgaccc gacaaatccc cggacatgtg caccaggcaa caggtgaacc agcgcggggc
      181 gaagctcaag ctgcttgcca agccggtgcg cgcccgacga aagaccgccg cacggtcgaa
      241 aggatagtgt ggtccagtta tctcaggcaa tagcggcgcc cactccaatg catgagtggg
      301 tgcaggacat ggtttgtttt ttggaattga tgtcggtgga aggggaggga ggatttacaa
      361 ttgggcgtgg atgattcggg tggttggatg atctgggaag gacttgtctg gagaactggt
      421 catggattcg acctcgaagc gcgcggctcg tgatgaaatc gcggacatgg tggtggactg
      481 gctcactcgg tcgagcaagc tgtctgaact ggcaacggcc gacctggaga cgattgagga
      541 tgaggtggta gagaatatcg atgccatcac gcggagtgct atctccaagc tgcttgatca
      601 acagcggcgg cagtcggaaa ccccgcaggc gtgcccgcaa tgcggtgggt gtttgagcgc
      661 caagccggct caacggcgca cgatgcaatc ccggcgcggc aacattcact tttcctgcga
      721 tgtgtttcat tgcccggcct gccgccggga tttttttccc tcagtttaga atcctgggtt
      781 gtgacgttga ccagacgcag acgccggccg tgctcaagaa ggtcgcgcgg gcggcagtgc
      841 gtcatggctc ctacgagaag gccagcgtca acctgcaaga catggccgac ttgaaggtgt
      901 ctgccaaaca agtgcagcgg gtggcgattc gcctgggcga agagcggctt gcggaacagc
      961 agcagcgtgt cgcccgttat caagccgcct cgttgcccga acggcagggc cagcctcccg
     1021 aagctccgcg aaataattgg tcaggccgcg ccgccgcgat tcaatgcgac ggcggacggg
     1081 cgcagattcg cgatgaactc tgggatgccg agaagccgcc gggcaagcgg catcgctggt
     1141 ggcgcgagac acagtcgggc gtgctgcaga catatctgtc ccagcccgca cagcaggatc
     1201 cgcacccgga agtgccggcg gaattgatgg atccgctctg ggcggtgcct aagttcaacg
     1261 agattcattg tgcccatgcg gccgccggtt cgcctgattc tccggcggaa acgggcgatt
     1321 ctgacgcgag tgctcgcgcg ggcgatgcct gcgcgagcga tgatccctcg gaacgctccg
     1381 aggccgcgcg gcggaaaggg aagggcgccg ccaagaacaa atcgaagaag aaaccaccgc
     1441 cgtggagcgg tggttcgccg ctggtcaaaa cggtcgtggc cacacgccgc ggatacgacc
     1501 atttggggct gttactggcg gccgaggcat tcatgcgcgg tttcaacctg gcgacggcca
     1561 aagcctttct gggagacggg ctcaaggtga actggacgat ctggagccgg cacttctcgc
     1621 actacacgcc gatcgtcgat ctgatgcatg ccttgagcta tgtctacgcc gcagcggtcg
     1681 cttgcagccc gacgatcgag gatggctgga gcatgtatct tcgctggctc gcttgggtct
     1741 gggccggcaa cgtctcgctg gtcttggagg cgatgcggga aatcgcggcc ggtagaacgg
     1801 accgcgtcga atccttggat cgagcgatca catatctcag caacaatgcc tcgcgaatga
     1861 agtacgccga gtaccgtcag gccggcttgc ccatcacgac cgccttgatt gaatccaccc
     1921 agaagcagat caacggccgc atcaaaggca cggaaaaatt ctggtgcgac gagcgtctgg
     1981 agcccctcct gcagctactg caagacgacc tcagcgacac ctacgacagc cagcagttct
     2041 ggcaccgtcg ccgccaacgc ttcaccggct tccgaaaccg ccgtgccaag
//
LOCUS       DASUGB010001071         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1648948, whole genome shotgun sequence.
ACCESSION   DASUGB010001071 DASUGB010000000
VERSION     DASUGB010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648948"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VFV87_20300"
     CDS             <1..1379
                     /locus_tag="VFV87_20300"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5106177.1"
                     /translation="EHDPTQKVAKAAPPWLISMVVHMILVIVLALMTIPLMLNDNQVQ
                     LEVVYAETLGEQVLDDKLQSPDSLEMNVDVPALSFDTKPADDPLAAPPLIDVNVLDAT
                     SATDSIQAPSIGLALSGREAGSKKALLAAYGGNATTEGAVRAGLEWLKKQQMKDGGWS
                     LTGPYPDGSGLADNRCSATAMALLAFQGAGHTHQSGEFKDVVRRGWDYLLKMQNADGY
                     FQHDGVQHHLLYAQAQATIAVCEIYGMTKDEKFKKPAQKALDFAHKAQAPEGGWRYEP
                     RIDSDTSVTGWYVMALQSGMMAGLEVQSPSLDQIHKFLDRCAQENGARYGYQPGRETT
                     PTMTAEALLCRQYLGWKHDDERLRRGIDYVLANPIDYTDQNVYYWYYATQACHHMDGP
                     DWNRWNAVMRQAVPENQVKTGNDRGSWSAAGDRWGSHGGRLYVTCLSIYMLEVYYRHL
                     PIYKWRLQ"
     gene            1768..>2217
                     /locus_tag="VFV87_20305"
     CDS             1768..>2217
                     /locus_tag="VFV87_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106178.1"
                     /translation="MLWGLLAALHATGCELAHFHSVSCLAPHDAARSLTSSASRHLDS
                     WAMSRAGCLRALYRTSQHAELAVVEGDFRVAASPVMPAPALAPASRPASSLGTLCDWL
                     DLPRVAIVDLADLDACRLPPRPERLDALLLDRAHDALHAAWWQTTLEA"
BASE COUNT          400 a          728 c          725 g          364 t
ORIGIN      
        1 aggagcacga tccgacgcag aaagtcgcca aggccgcgcc gccgtggctg atcagcatgg
       61 tcgtccacat gatcctggtg atcgtgctgg ccttgatgac gatcccgctg atgctgaacg
      121 acaaccaggt gcagctggaa gtcgtgtatg cagaaacgct gggcgagcag gtgctggacg
      181 acaagctgca gtcgcccgat tcgctggaga tgaacgtcga tgtgccggcc ctgtcgttcg
      241 acaccaagcc ggccgacgat ccgctggccg cgccgccgct gatcgatgtg aacgtgctcg
      301 atgccacgtc ggccaccgac agcatccagg cgccgagcat cggcctggcc ctgtccggcc
      361 gcgaggccgg gagcaaaaag gcgttgctgg ccgcctatgg cggcaacgcc accaccgaag
      421 gggccgtgcg ggccggactg gaatggctga aaaaacaaca gatgaaggac ggcggctgga
      481 gcctcaccgg cccctatccc gacggcagcg gcctggccga caaccgctgc tccgccacgg
      541 cgatggccct gctcgcgttc caaggcgcgg gacacacgca ccagtcgggc gagttcaagg
      601 acgtcgtccg ccgcggttgg gattacctgc tcaaaatgca aaacgccgac ggctacttcc
      661 agcacgacgg ggtccagcac cacctgctct atgcccaggc gcaggccacg atcgccgtgt
      721 gcgaaatcta tggcatgacc aaggacgaga aattcaagaa gccggcgcag aaggctctcg
      781 acttcgccca caaggcccag gcgcctgaag ggggttggcg ctacgagccg cggatcgact
      841 cggatacgtc cgtcaccggc tggtacgtca tggccctgca aagcggcatg atggcgggcc
      901 tggaagtgca aagcccgtcg ctcgaccaga ttcacaagtt cctcgaccgc tgcgcccagg
      961 aaaacggcgc ccgctacggc tatcagccgg gccgcgaaac cacgccgacg atgaccgccg
     1021 aggcgctttt gtgccggcag tatctcggct ggaagcacga cgacgagcgc ctccgccgcg
     1081 gcatcgatta cgtcctggcc aacccgatcg actacaccga ccagaacgtc tattactggt
     1141 attacgccac gcaggcctgc caccacatgg acgggccgga ctggaaccgc tggaacgcgg
     1201 tcatgcggca ggcggtgccc gagaatcagg tgaaaacggg caacgaccgg ggaagctggt
     1261 cggccgcggg cgaccgttgg ggctcgcacg gcggccggct gtacgtcacc tgcctctcga
     1321 tctacatgct cgaggtctat taccgccatt tgcccatcta caagtggcgg ctgcagtgag
     1381 ggcccggacc gaggttgaag atctggcgtc ggagttcgag attgtgggcg caagccatgc
     1441 gatctgcctt cttgtacact tctcgcgcgg gggccgatgc ggcaagcgga gcaggatttt
     1501 gagctatttt cggtcgccgg ccctgcttga ctctggtatc gcgtaggcga agaatcaaag
     1561 tgcattcgga cactacaccg gatgagggcg gatcagccct tccttgattt ctctccctga
     1621 atctaagctg atctgctgcg ggcagtcggg cagtcgggca gtcgggcatc gagcagtcgc
     1681 gcaccaacgc ctaccgggtg accgtttatg aggcctttgc cgcgcgtcgc tgtcggaacg
     1741 atccaaccgc acgccgagcg ccagcccatg ctctggggct tgctcgcggc cctgcacgcc
     1801 acgggctgcg agctggcgca tttccattcg gtctcctgcc tggccccgca cgatgccgcg
     1861 cggtcgctga ccagttccgc cagccgccac ctcgacagtt gggccatgag ccgcgcgggc
     1921 tgcctgcggg ccctgtatcg gacgagccag cacgcggagt tggctgtcgt ggaaggggat
     1981 ttccgcgtcg cggcttcgcc agtaatgcct gcacctgccc tggcgccggc gtcacggccc
     2041 gccagcagcc tgggaaccct gtgcgattgg ctggatctgc cgcgcgtggc aattgtcgat
     2101 ctggccgact tggatgcctg ccggctgccc ccacggcccg agcggctgga tgccctgttg
     2161 ctggaccggg cgcacgacgc cctgcacgcc gcctggtggc agacgacgct ggaggca
//
LOCUS       DASUGB010001072         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1091885, whole genome shotgun sequence.
ACCESSION   DASUGB010001072 DASUGB010000000
VERSION     DASUGB010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091885"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1105)
                     /locus_tag="VFV87_20310"
     CDS             complement(218..1105)
                     /locus_tag="VFV87_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5106179.1"
                     /translation="MTSPAVFDQLSAADVQRIREVLRVEMPDGPAASAETPLDRIEIA
                     QRLLGPELCRKLGIYRLPADFKLSVVIPVYNEIRTLAKIIERVRATRLPLEIVIVDDG
                     SRDGSREYLAELRDAGGEQNRDLKIVLHERNQGKGGALKTGFLECTGDAVIIQDADLE
                     YDPQDYRSLLQPLVEEHADVVYGSRFSHIDGPVHNYWHRWGNKVITRLSNWKTGRAWT
                     DVETCYKLIRRDLLQRIAPSLQERGFGIELELTAKLARQPGVRFFERPISYTGRSWAE
                     GKKIGIKDGLWAMWCILRY"
     gene            1779..3086
                     /gene="panC"
                     /locus_tag="VFV87_20315"
     CDS             1779..3086
                     /gene="panC"
                     /locus_tag="VFV87_20315"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5106180.1"
                     /translation="MGLGSNLGERVGSLTNALVRLRADPHVRVLRSSSLLETRPVGGP
                     EGQPRFLNAAAVLETDLPAEQLLERLLAIERELGRMRGEQWGPRTIDLDLLLYDGQVI
                     NTASLTVPHPRLAERRFVLEPAAEIARELRDPASGLSIGELLERLGPPACGEMGLRIY
                     LEPRAMRRAAESLRREGKRISLVPTMGALHEGHLSLVREARQRSDVVIATIFVNPTQF
                     GPQEDFSRYPRTLEGDLRALADHGCDLAFVPEQSAMYPAGFSTYIEPPAVAQPLEGVC
                     RPGHFRGVATIVLKLFQIVPADIALFGQKDYQQSLVIRRMALDLNVPIEIATCPTVRE
                     PDGLAQSSRNRYLSPAERQQALALSRSLDLAERLVKSGERGTAAIVEQMRAELRTAGI
                     ERIDYVSVADPETLVEQTRIVLPAVALVAAYAGTTRLIDNRIL"
     gene            3094..>4233
                     /locus_tag="VFV87_20320"
     CDS             3094..>4233
                     /locus_tag="VFV87_20320"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5106181.1"
                     /translation="MRSTLFYIPAEVAGVPVFGVGWLLGIWLVLCIAILFWIWRQTGD
                     SRELVGYLPFLAIVALVIVFMLPMMMQLGSDGQPLGVPIRGFGVMFMLATVAGVGLAA
                     WRARQMGIDPDVIYSLAFWMVIAGLIGARLFFVVQYWHEFQKPTVGETIQSLMNFTKG
                     GLVVYGSVLTGLPVGIWYLRKRGLPILAVADLIAPSMVVGLALGRIGCFMNGCCFGGE
                     CLPPDWRAMTFPVDSPPYQQQITRGWMSGVWLAERDEQIVVNYVAPGSPAEAAGVRIG
                     DAIASINGAAVTSLDEARDRLMPESAVGTKFPRAFVIETTDGRTLDWLVNQPPQRTVP
                     IHPTQLYAALDAALLALVLWFYYPFRRRDGEVFALLITIHPLSRFL"
BASE COUNT          740 a         1272 c         1391 g          830 t
ORIGIN      
        1 ataggccagc acccgcatcg ggcaacgctc gtgggccgca tcgaagagcc gcagaaacgc
       61 ggcgtaatcc tcatccttgt gaaagacggt ctggcgagag ttgccacggc tgatgacgtg
      121 gtaacagaac ccgccggggg cgtgtcgttt gggcctgggc atgcggggga atgtaccgcg
      181 cccagcctgg cgccgcaata atgaggcctg acccttttta gtagcgcaga atgcaccaca
      241 tggcccagag gccgtctttg ataccgatct ttttgccttc ggcccaggag cggccggtgt
      301 agctgatggg gcgctcgaag aagcggacgc cgggctgccg ggcgagcttc gccgtcaatt
      361 ccagttcgat gccgaagccg cgttcttgca ggctcggcgc gatgcgctgc aacaggtcgc
      421 ggcggatcag cttgtagcac gtttccacgt cggtccaggc ccggccagtc ttccagttcg
      481 acagtcgtgt gatgaccttg tttccccagc ggtgccagta gttgtggacc gggccgtcga
      541 tgtggctgaa gcggctgcca tagacgacat cggcgtgctc ttcgacgagc ggctgaagga
      601 gcgagcggta atcctgagga tcgtattcga ggtcggcatc ctgaatgatg accgcatcgc
      661 cggtgcattc gagaaagccc gtcttgagcg cgccgccttt gccctggttg cgctcgtgca
      721 gcacgatctt caggtcgcga ttctgctcgc cgcccgcgtc gcggagctcg gccagatact
      781 cgcgcgagcc gtcgcggctg ccgtcatcga cgatcacgat ttcgagcggc aaccgtgtgg
      841 cccgaacccg ctcgatgatc ttcgccagcg tgcgaatctc gttatagacg gggatgacga
      901 ccgagagctt gaaatccgcg ggaaggcggt agatccccag cttccggcac aattccggcc
      961 ccagcaggcg ctgcgcgatc tcgatgcggt ccagcggcgt ttcggcagac gcagcagggc
     1021 catccggcat ttcgacacgc aagacttcgc ggatgcgctg cacgtcggct gcggaaagct
     1081 ggtcaaaaac agcaggagaa gtcatggaag ccgggcattg atgcagcaca ggtgtcgcca
     1141 agtctagtgt accgcacgaa tcgccacgcg gcttgcggcg cgcaacagtc gcctttcgct
     1201 ccgcgaaagg gcgttcgttc gcggagcgaa cgacgacatt aacgcacagc cgtttcgaca
     1261 atcgtttgcc gctgccagcc gatgctagca gcgcgaaaaa aatcttcggc ccaccgcgtt
     1321 gacaagtttt ttgcggctgg cacaatacgg ccccgttggt gatcggtggt cgcgcagcga
     1381 tgccgagata ctggcggcga gcagcaacgg cgcagccaga atcagaatcg attcgtggcg
     1441 tggattgacc aacgatctgc ttttcgttca ttcaacgtgc tggtgttcgc aacaagaggc
     1501 catcgctcgc agccattctg gcgtgcgagt atgcctggca agtcccacga tgcgtcagcg
     1561 cctgcgcgct tcgcacaggc cgcgagtaat ttcaagagat tctgaaactt cgagcagcga
     1621 tctctcatgc acctgggggc cgatgaccga gcggaacgac agtcggcgac ctggtcgagg
     1681 aacggacgcc cccccttttt gcttcttcaa cttcacgcac gcagccgtac gcaattgacg
     1741 ccaagggata taatcgcctg aatatggcga attgtctcgt gggactcggc tccaatctcg
     1801 gtgagcgagt tggctctctg acgaacgcgc tggtgcggct gcgggcggac ccgcatgttc
     1861 gagtgctgcg cagcagctct ttgcttgaga caagacccgt cggcggcccg gagggtcagc
     1921 cgcggtttct caatgcggct gctgtcttag aaacagacct gccggccgaa cagttgttgg
     1981 aacgactgct ggcgattgag cgcgagctgg gccggatgcg cggtgagcaa tgggggccgc
     2041 ggacgatcga tctggacctg ctcttgtatg acggacaggt tatcaacacg gccagcctga
     2101 ccgtgccgca tccgcggttg gcggagcgga gatttgtttt ggagcccgcg gcagagatcg
     2161 cgcgggaact gcgcgatcct gcgagcggct tgtcgatagg tgagctgctg gagcgattag
     2221 ggcctccggc gtgcggcgag atgggtctgc ggatctatct ggaaccgcgg gcaatgcgac
     2281 gggcggccga aagtctccgg cgggaaggca agcgcatttc gctcgtaccg accatgggcg
     2341 cgcttcatga ggggcacctc agcctggtac gggaggcgcg ccagcgatcc gacgtggtga
     2401 ttgcgacgat cttcgtcaat ccgacgcagt tcggtccgca agaagacttc tcccgttatc
     2461 cgcgaaccct ggaaggggat ttgcgggcgc tggccgatca tggctgcgat ctggcgttcg
     2521 ttcccgagca gtccgcgatg taccccgcgg gattctcaac ctatatcgag ccgcccgcgg
     2581 tggcccagcc gctggaaggc gtctgccggc cggggcattt ccgcggcgtt gccacaatcg
     2641 tcctcaagct gtttcagatc gtgccggcgg acatcgccct atttggccag aaggactatc
     2701 agcagtcgct ggttatccgg cggatggccc tggatctgaa cgtgccgatc gagatcgcga
     2761 catgccccac cgtccgcgag ccggacggtc tggcccagag cagccgcaat cggtatcttt
     2821 cccccgccga gcgacagcag gcgctggcct tgtcgcgaag cctggacctg gcagagcgac
     2881 tggttaagag cggcgaacga ggtacggctg caatcgtcga gcagatgcga gccgagttga
     2941 gaactgccgg catcgagcga attgattacg tctccgtcgc cgatccggaa acgcttgtgg
     3001 agcaaacccg gatcgttctc ccggccgtcg cactcgtagc ggcctatgcc gggacgacgc
     3061 gacttatcga caaccgaatc ctgtaggtaa acgttgcgat ctacgctgtt ctatattccc
     3121 gcagaagtgg ccggcgtgcc ggtctttgga gtcggctggc tgctcggtat ctggctcgtc
     3181 ctctgtatcg cgatcctgtt ctggatctgg cggcaaacgg gcgacagccg cgagctcgtc
     3241 ggctatctgc cgttcctggc gatcgtggcg ctggtcatcg tcttcatgct gccgatgatg
     3301 atgcaactcg gctccgacgg acagccgctg ggcgtgccga tccgcggctt cggggtgatg
     3361 ttcatgctgg cgacggtcgc gggggtcggc ctggcggcgt ggcgagcgcg gcaaatggga
     3421 atcgatccgg acgtgatcta ctcgctcgcg ttctggatgg tgatcgcggg cctgatcggg
     3481 gcccggctgt tctttgtagt gcagtattgg catgagttcc aaaagccgac ggtcggcgag
     3541 acgattcaat cgctcatgaa cttcaccaag ggcggcctgg tcgtgtacgg ctcggtcctc
     3601 acgggtctgc cggtcggcat ctggtacttg cggaagcgcg gcttgcccat tctggcggtg
     3661 gccgacctca tcgcacccag catggtcgtt ggactggcac tggggcgaat cggctgtttc
     3721 atgaacggct gctgcttcgg gggcgagtgc ctgccgcccg attggcgggc aatgacgttc
     3781 cccgtcgata gcccaccgta ccagcagcag atcactcgcg gctggatgag cggtgtgtgg
     3841 ctggccgagc gcgacgagca gatcgtggtc aactatgtcg cccccggcag tcccgccgaa
     3901 gctgcgggcg tgcgtatcgg cgacgccatc gcgagcatca acggcgcggc agtcacatcg
     3961 ctcgacgaag cgcgcgaccg gctcatgccc gaatcggcgg tggggaccaa gttcccgcgg
     4021 gcttttgtga tcgagacgac ggatgggcga acgctcgact ggctcgtcaa ccagccgccg
     4081 cagcggacgg tgccgattca tccgacgcag ctctacgcgg ccctcgacgc ggcgcttttg
     4141 gcgctggtgc tgtggtttta ctatccattc cgccggcgcg acggcgaagt cttcgcgctg
     4201 ctcattacaa tccacccgtt atctcgtttc ctg
//
LOCUS       DASUGB010001073         8067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1092076, whole genome shotgun sequence.
ACCESSION   DASUGB010001073 DASUGB010000000
VERSION     DASUGB010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092076"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..2711
                     /locus_tag="VFV87_20325"
     CDS             111..2711
                     /locus_tag="VFV87_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEX5106182.1"
                     /translation="MTVIQRFTRGGRALGAAGLLCAALAGCSVSNSTERQAATPPAAP
                     PAAPAVEAASATLDLPDEAALALAAQPGIATTPPASEPTATPAAAPAEPQDEVPIPAE
                     VTAAEPPPEATSFAADPPAESGPSAATEQPETALIARAPEEETPAESQPAADRTGPPP
                     SEPKASSPKAGDPPLKQANPFRRRLEAPEFPKNLEWLNTKPLTKADLKGKFVLLDFWT
                     YCCINCMHILPELKKLEKQYPNELVVIGVHSAKFETEKLSDNIREAILRYEIVHPVIN
                     DAEHKIWEEYGVSSWPTILMIDPEGNAVWGRSGEFKAEEVNEILKVAIPFYRAEKLLD
                     EKPLKFDLEVAKEPATPLRFPGKILADEKGNRLFITDSNHNRIVIATLEGRLLEVIGS
                     GAIGRADGDFRTATFDHPQGCALLDETLYVADTENHLLRKVNLRAKTVSTIAGTGKQA
                     EHAWPGLENAQASEQLPERWVGPPSATAINSPWALWIHQEDLYIAMAGPHQIWRMPLN
                     EKEIGPYAGNGREDIVDGPLLPRIPYAQGFSSFAQPSGLSSDGTWLYVADSEGSSIRA
                     VPFDPKKHVKTVVGSDHLDSGRRLFAFGDRDGAKRVAKLQHCLDVVHHEGKLYVADTY
                     NHKIKVVDAKNGSVKTIVGTGEPGAADEPAQFHEPAGLAYANGKLYVADTNNHLIRTI
                     DLASGTVATLTIAGLMAPAGKLAVNSAVGGQGGGNDGPPAAGAATAAPKKPSFKGAHQ
                     EKLTPTTVKPIDGTATFRVSLKVPDGWKINQLAPMSFWLDSPRESGAADRAAFGRVKL
                     AKPVAEFDVPVKISGEGEDELQVSLNYNYCRDGDNGVCKFGAVVFTVPLKIAATGKPE
                     LVQLIHEIPE"
     gene            2708..3676
                     /locus_tag="VFV87_20330"
     CDS             2708..3676
                     /locus_tag="VFV87_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5106183.1"
                     /translation="MSQRVADVSSFAKRVIANVERAIVGKRPQLILSLVSWLCEGHIL
                     LEDVPGVAKTMLARALARSVGCAFKRIQCTPDLLPSDVTGASIFNQKTTEFEFRPGPI
                     FAQVVLADEINRATPRTQAALLEAMAESRVTVDGLTYKLGPPFLLIATQNPVDHEGTF
                     PLPEAQLDRFLMRFSLGYPTQEEELRMLELLQHSHPIDALESVSNAEEVVACQQAVRE
                     IYVDDKVRRYLMQIVHDTRSHDDIGLGASPRASIALFRTSQAMAALRGRNYVLPDDVK
                     RVAGPVLTHRVILRPESRLRKITAGAVVDEIVAEIAVPTLEERAAS"
     gene            complement(3892..4245)
                     /locus_tag="VFV87_20335"
     CDS             complement(3892..4245)
                     /locus_tag="VFV87_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106184.1"
                     /translation="MIEPLLLPEPLLLEPQDSSRTAAGPILEAFQDRLQTLISQLEEQ
                     VHMIRHDGVLEHRPCAESRSVLNRLDHHLGHAGIGQIGAPLGAVEPGFYFGEQLPLPF
                     QAISVLLAADAAQFG"
     gene            4446..5753
                     /locus_tag="VFV87_20340"
     CDS             4446..5753
                     /locus_tag="VFV87_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5106185.1"
                     /translation="MIWFAGAILLLVIALVFNLPLLAYAMYVLLAVLLVSRFLTRRWA
                     ESLAATRECNQLSAEIGQSVAVMINVKNQGAWPVAWVLIEDLLPRDALMYRPPRLEVS
                     GRRVSLMMLNGRAKKTLLYQLTPTRRGYYQLGPLVLETGDLFGLHRRYRVATDPHFLL
                     VLPKVLPLAGYEISSKRPIGEVRMSYRLYEDPTRISGVRAYERGDSLNRIHWRATART
                     GLLHSKVYEPSTVAGATLLVEFHQAAHDPRDEPHRSELAITAAASIANAVYLMNQQVG
                     LITNGRDAVDRIRHEGWAHDYRSREAALASAAVREKSDRLLPLVVPTRRGPEQFTRIL
                     ESLARLELTDGLLLPALIEETAGRMPRDATVIAIVPPKSENAALALGNLRRRGFAVTA
                     LLNLYEDYDYAQLAGPFLAEGIETRHLRDEQSILEICRSYALR"
     gene            5950..7266
                     /locus_tag="VFV87_20345"
     CDS             5950..7266
                     /locus_tag="VFV87_20345"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106186.1"
                     /translation="MLTFTTGKSRDCDGVNRRSFLQVGALAGLGVSLPMLFSAKARGA
                     GGGSGANCILIWTRGGTSHHDTFDPKPEAPVSVKGEYGVIDTAVPGVKFAEIVPTFAK
                     ELNRFSLLRGWNPQNGSHGTADQYVMSGRKFNQAVRSPTYGSVVSYELGFKTAMPPFV
                     QLGTEVDERFGGGSPGILGLEHGAFTIPADPSADKFTVQDITPPEGITSERIEFRKKV
                     LARIDQLQRRGDLQPAAFDAIDESYKTAFDMITAPETKGAFDLSQEKPELREAYGKNK
                     FGQSCLLARRLIEAGVRFVTVTDGAWDTHANNFKSLKERLVPRVDQALPRLLIDLEER
                     GLLSTTLVIWLTDFGRTPQINAASGRDHWASAGFAIAAGAGIPGGAVVGQTDGEGGYV
                     TKDEYHSEDIATTLYAKLGIPTDLIAHAPDGRPVRLIEGREIKEWM"
     gene            7353..7778
                     /locus_tag="VFV87_20350"
     CDS             7353..7778
                     /locus_tag="VFV87_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX5106187.1"
                     /translation="MTAVDLVSIQDKESSVRSVILAQTTLLLIASNVFMTFAWYGHLK
                     SLNHKAWYIAALISWGIALFEYLLQVPANRIGHTQFNVAQLKILQEVITLSVFVPFSI
                     WFLREPIKLDYLWAALCLCGAVYFVFRHELSAIILPVSH"
     gene            7826..>8067
                     /locus_tag="VFV87_20355"
     CDS             7826..>8067
                     /locus_tag="VFV87_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106188.1"
                     /translation="MLSTDTQLVQIRWLIVATLAALTILVTVRLWMVEGLVLRPVSID
                     GPSMAPALVGLHYAVTCDDCAFAFACDAQNAPASGQV"
BASE COUNT         1521 a         2643 c         2505 g         1398 t
ORIGIN      
        1 ggtgtaaaag cagtcaccgc tagcagaaaa gcggttcacc cgcgctcgcg gaggcagtac
       61 catgaatggg ccatagtaag ggctagtcgg gactcttgtg aggtcacacc atgacggtaa
      121 tccaaagatt tacccgcggc ggtcgtgcac ttggtgctgc gggcctgctt tgcgcggcgc
      181 tggcgggctg tagcgtttcc aactcgaccg agcgacaagc agcaactccc ccagccgctc
      241 ccccagccgc tcctgcggtc gaagccgcct ctgcgacgct cgatttgccg gacgaagcgg
      301 ccctggcttt ggccgcgcag ccagggattg ccacgacgcc gcccgccagc gagccgaccg
      361 caacgcctgc tgccgcacct gcggagcccc aggatgaggt ccctataccg gcggaagtaa
      421 cagcggctga gccgcccccg gaagccacgt ccttcgccgc cgatcccccc gcggaatctg
      481 gtccttctgc tgccaccgag caacccgaaa cggctcttat agcccgcgcg cctgaggaag
      541 aaacccctgc cgaatctcag ccggctgccg accggaccgg tccacctcct agcgagccaa
      601 aggcctcgtc ccccaaagct ggtgacccgc cactcaagca ggccaacccg ttccgtcgcc
      661 gcctggaggc gcccgagttc cccaaaaacc tggaatggct caacacgaaa ccgttgacca
      721 aggcagacct gaagggcaag ttcgtcctgc tcgatttctg gacctactgc tgcatcaact
      781 gcatgcacat cctgcccgag ctgaagaagc tagagaagca gtatcccaac gagttggtgg
      841 tcatcggcgt ccattccgcc aaattcgaga cggaaaagct cagcgacaac attcgcgagg
      901 cgatcctccg gtacgagatc gtccacccgg taatcaacga cgcggagcac aagatttggg
      961 aagaatatgg cgtcagcagt tggccgacaa tcttgatgat tgaccccgag ggcaacgccg
     1021 tctggggccg cagcggcgaa ttcaaagccg aggaggtcaa cgagatcctc aaggtggcga
     1081 ttccctttta ccgcgctgaa aaactgctcg acgagaagcc gctcaagttc gacttggaag
     1141 tcgccaagga acccgccaca ccgctgaggt ttcccggcaa gattctggcc gatgaaaagg
     1201 gaaaccgcct gttcatcacc gacagcaatc acaaccgcat cgtgattgcg acgctggaag
     1261 ggcgactcct ggaagtcatc ggcagcgggg caataggccg ggccgacgga gattttcgaa
     1321 cggcaacgtt cgatcatccg cagggttgcg cactactcga cgagacgctg tacgtcgccg
     1381 ataccgagaa ccacctcctc cgcaaagtga atttgcgggc caagaccgtg agcacaatcg
     1441 ccggcaccgg caaacaggcc gagcatgcct ggcccgggct cgaaaacgca caagcgtccg
     1501 agcagcttcc cgagcggtgg gtagggccgc cctccgccac cgcgatcaac agcccgtggg
     1561 ccctgtggat ccaccaggaa gacctgtaca tcgccatggc tggcccacac cagatttggc
     1621 ggatgccgct caatgagaag gaaatcggcc cctacgccgg caacggccgc gaggatatcg
     1681 tcgatggtcc gctcttgccg cgaattccct acgcgcaagg tttcagttcg tttgcccagc
     1741 cgagcggtct ttcgtccgac gggacctggc tctacgtcgc cgatagcgaa ggcagctcaa
     1801 ttcgggccgt gccgttcgat ccgaagaagc acgtgaagac ggtcgtcggc agcgaccacc
     1861 tcgattccgg ccgtcggctg ttcgccttcg gcgaccgcga cggggccaag cgcgtcgcca
     1921 agctccagca ttgcttggac gtcgtgcatc acgaaggcaa gctctatgtc gccgacacgt
     1981 acaaccacaa gatcaaagtg gtggacgcca agaatggttc ggtcaaaacg atcgtcggca
     2041 ccggcgagcc tggcgctgcc gacgagcccg cccagttcca cgagcccgcc ggcctggcct
     2101 atgccaacgg caagctgtac gtggccgaca cgaacaacca tctcatccgc acgatcgatc
     2161 tggccagcgg cacggtcgcc acgctcacga ttgccggctt gatggcacct gccggaaagc
     2221 tcgcggtcaa ttccgcggtg ggcgggcagg ggggcggaaa tgacggcccg ccagcggccg
     2281 gcgccgccac tgccgctccc aagaagccca gctttaaggg cgctcaccag gagaagctca
     2341 cgccgacaac ggtgaagccg atcgacggca cggccacgtt ccgcgtttcg ctcaaggttc
     2401 ctgacggctg gaagatcaac cagctcgcgc cgatgtcgtt ctggctcgat tcaccccgcg
     2461 aatcgggcgc cgctgaccgc gccgccttcg gccgcgtcaa acttgccaag ccggtcgccg
     2521 agttcgacgt gccggtgaaa atcagcggcg agggcgaaga tgaactccaa gtgtcgctga
     2581 attacaatta ctgccgggac ggggacaatg gggtttgcaa gtttggcgcg gtcgtattca
     2641 cggtgccgct gaagattgcc gcgaccggca agcccgagct ggtgcagctc atccacgaga
     2701 ttcccgagtg agccagcgcg tggccgacgt cagcagtttc gccaaacggg tcatcgccaa
     2761 tgtcgagcgg gcgatcgtcg gcaagcggcc gcagctcatt ctctcgctcg tctcgtggct
     2821 ctgcgagggg catattttgc tggaggacgt gccgggtgtc gcgaagacga tgctcgcccg
     2881 agccctggcc cgctcggtcg gctgcgcgtt taagcggatt caatgcacgc cggacctttt
     2941 gcccagcgac gtgaccgggg cgtcgatctt caatcagaag acgacggagt ttgagttccg
     3001 ccccgggccg atctttgccc aggtcgtgct ggccgacgaa atcaaccgcg ccacgccccg
     3061 cacgcaggcc gcgctcctcg aagcgatggc cgaaagccgc gtcacggtcg atgggctgac
     3121 ctacaagctc gggccgccgt tcctcctcat cgccacgcaa aacccggtcg atcacgaagg
     3181 gaccttcccg ctgccggagg cacagctcga ccgcttttta atgcggttca gcctcggcta
     3241 tccgacgcag gaggaagagc tgcggatgct ggagctgctg cagcactcgc acccgatcga
     3301 cgccctggag tcggtgtcga atgccgagga agtcgtcgcc tgtcagcagg cggtgcgcga
     3361 gatttacgtt gatgacaagg tccgccgcta cctgatgcag atcgtgcatg atacgcggtc
     3421 gcacgacgac atcggattgg gggccagccc aagggcctcg atcgccctgt tccgcacgtc
     3481 gcaagcaatg gccgccctcc gcggccgcaa ctacgtgctg cccgacgacg tgaagcgcgt
     3541 cgctggcccc gtcctcaccc accgcgtgat cctccgcccc gaaagccgcc ttcgcaaaat
     3601 caccgccggc gcggtcgtcg atgagatcgt cgccgaaatc gccgtgccga cgcttgaaga
     3661 gcgagcggca tcgtaagtag gtcccgacgg ccgcggcggg accagcgccg cgacaggacg
     3721 tcgaccgccc taactaaaca acgctccaaa gacatgactt gtaagcccga aaccatcgac
     3781 ttctgcgtcg gccgtctgcc gcactgggaa gtcgtcgatg gccgctactt cgtgacgatc
     3841 cacctgtcgg gcgccatccc tcgggccgga cagcagcgta tccgggagat ctcagccgaa
     3901 ctgcgccgca tcggccgcca gaagcacgct gatcgcttga aatggcagcg gaagttgttc
     3961 gccgaaatag aagcctggct cgaccgcgcc gagcggcgca ccgatttggc cgatccccgc
     4021 gtggccgaga tggtggtcga ggcgattgag caccgagcgc gactcggcgc atggacgatg
     4081 ttcgagtacg ccgtcatgcc gaatcatgtg cacttgttct tcgagttggg aaatgagcgt
     4141 ttgaagcctg tcttggaagg cttcaaggat tggaccggca gccgtgcggc tcgaatcctg
     4201 cggttcgagc agcagcggtt ctggcagaag gagtggttcg atcactggtc gcgctcggat
     4261 gaggaagacg agcgaatcgt ggagtacatc cggcagaatc cggttcgggc gcgtctcgtt
     4321 tcggactgga gccaatggcc gtatgggtcg tggagttcga tggcctctgc cttgcgcgcc
     4381 gatcggccgc cgccaggtcc actcgcagtt ccggtcccgc cgaggccgtc gggacctaca
     4441 ttctcatgat ctggttcgcc ggcgcaatct tgctgctcgt catcgcgctg gtgttcaacc
     4501 tgccgctcct cgcctacgcg atgtatgtgt tgctggcggt gctgctggtc agccggttcc
     4561 tgacgcggcg gtgggccgag tcgctggcgg ccacgcggga gtgcaaccag ctctcggcag
     4621 aaatcggcca atcggtcgcc gtgatgatca atgtgaaaaa ccaaggcgcc tggccggtgg
     4681 catgggtcct gatcgaggac ctgctgccgc gggatgccct catgtaccgg ccaccgcggc
     4741 tggaggtgag cggccgccgc gtgagcctga tgatgctcaa tggccgggcg aaaaagacgc
     4801 tcctctatca actcacgccg acccgccgcg gatactatca gctcggaccg ctggtgctgg
     4861 agacggggga cctgttcggc ctccaccgcc gctaccgcgt ggcgaccgac ccgcatttcc
     4921 tgctcgtgct gccgaaggtg cttccgctgg cgggatacga gatttcgtcc aagcggccga
     4981 tcggcgaagt ccgaatgagc tatcggctct atgaggatcc gacgcggatc tccggcgtgc
     5041 gggcctatga gcggggcgat tcgctgaacc gcatccattg gcgggcgacg gcgcggacgg
     5101 gactcctgca cagcaaggtc tatgagcctt cgacggtcgc cggggccacg ctgctcgtcg
     5161 aattccatca ggccgcccac gatccgcgcg acgagccgca ccgcagcgag ctggccatta
     5221 ctgccgcggc gtcgatcgcc aacgccgtct atctgatgaa ccagcaggtg ggcctgatca
     5281 ccaacggacg cgatgccgtc gatcgcatcc gccacgaagg ctgggctcac gattaccgct
     5341 cgcgcgaggc ggccctcgcc tccgccgccg tccgcgagaa gagcgaccgc ctgctgccgc
     5401 tcgtcgtgcc gacgcgccgc ggcccggagc aattcacgcg gattctcgaa tcgctcgccc
     5461 gcctggaact gaccgacggc ctgctgctgc cggccttgat cgaagaaacc gccggccgca
     5521 tgccccgcga cgccaccgtc atcgccatcg tgccccccaa atccgagaac gccgccctgg
     5581 ccctcggcaa cctcaggcgc cgcggcttcg cggtcactgc cctactcaat ctttatgagg
     5641 actacgacta cgcccagctc gccggcccct tcctggccga aggaatcgag acgcggcacc
     5701 tgcgggacga gcagtcgatc ctggaaatct gccgcagcta tgccctccgc taacgactcg
     5761 caaattgcca caacgaccga caacgaccgt cgagccgttg ccttttggcc gataactatg
     5821 gtaggatcaa ccagcttgga tgggtcggtt cccgcctgaa tttcccacct gaaaaactgc
     5881 agtgaatctc gtccatggcc gtcattcgcg gctggtggcg tgctgcatcc ctccctgaag
     5941 gaactgaaca tgctcacgtt caccaccggt aagtcgcgcg attgtgacgg cgtcaaccgc
     6001 cggagttttc tgcaggttgg ggcgctggcc gggctgggcg tttcgctacc gatgctgttt
     6061 tcggccaagg cccgcggggc cggcggcggg agtggtgcga attgcatctt gatctggacc
     6121 cgcggcggca cgagccacca cgataccttc gatcccaagc ccgaagcgcc ggtcagcgtg
     6181 aagggggaat acggcgtgat cgacacggcg gtccccggtg tcaagtttgc cgagattgtg
     6241 ccgacgttcg ccaaggaact caatcgcttc tcgctcctcc gcggctggaa tccgcaaaac
     6301 ggcagccacg gcacggccga ccagtacgtg atgtcgggcc gcaagttcaa ccaggctgtg
     6361 cggtctccga cgtacggctc agtcgtgagc tatgagctgg gcttcaagac ggcgatgccc
     6421 cccttcgtgc agctgggaac cgaagtcgat gagcggttcg gcggcggcag cccgggcatt
     6481 ctgggcctgg agcacggcgc attcacgatt cccgctgatc cgagcgccga caaattcacc
     6541 gtgcaggaca ttacgccccc cgaaggcatc acctctgagc ggatcgagtt tcgcaagaag
     6601 gtgctggccc ggatcgacca gctccagcgc cgcggcgatc tgcagccggc ggcgttcgac
     6661 gcgatcgacg agagctacaa gacggctttc gacatgatca ccgcgccgga aacgaaaggc
     6721 gcgttcgacc tttcgcaaga aaagccggag ctgcgcgaag cctatggcaa gaacaagttc
     6781 ggccagagct gcctcttggc ccggcggctg attgaggcgg gggtgcggtt tgtcaccgtc
     6841 accgacggcg cgtgggatac gcacgccaat aacttcaaga gcctgaagga gcggctggtg
     6901 ccgcgcgtcg atcaggcgct gccgcggctg ctcattgacc tcgaagagcg gggcctcctg
     6961 agcacgacgc tggtgatctg gctgaccgat ttcggccgca cgccgcagat caacgccgcc
     7021 agcggccgcg atcactgggc cagcgctggc ttcgccatcg ccgccggtgc aggaatccca
     7081 ggcggcgcgg tcgtcggcca gaccgacggc gaaggaggct atgtcaccaa ggacgagtac
     7141 cactccgaag acatcgccac cacgctctat gccaagctcg gcatccccac cgacctgatc
     7201 gcccacgccc ccgacgggcg ccccgtgcgg ctgatcgaag ggcgggagat taaggagtgg
     7261 atgtagaaag tccagcttta accggagctt gactgccaac ggcctttcgg ccacctggcc
     7321 gtgcccggat ataatggcac cgaaatacag ccatgacggc tgtcgatctg gtttccatcc
     7381 aggacaagga gtcatccgtg cgctcggtga ttctggctca aaccactctc ctgctcatcg
     7441 cgtcgaatgt gttcatgacc ttcgcctggt acgggcacct caaaagcctg aaccacaagg
     7501 cctggtacat cgcggcgctc atcagttggg gcatcgccct gttcgagtac ctgctgcagg
     7561 tgccggcgaa tcgcatcggc cacacgcaat tcaacgtggc ccagctcaag attctgcagg
     7621 aagtgatcac gctcagcgtg tttgtcccgt tctcgatctg gttcctgcgc gagccgatca
     7681 agctcgacta cttgtgggcc gccctgtgcc tgtgcggagc ggtttatttt gtgtttcggc
     7741 acgaactgtc ggcgatcatc cttcctgttt cacactagcg atgattccgg agcaatccag
     7801 ccgagctgcc gaccagacgt cggccgtgct ttcaacggac acgcagttgg tgcagatccg
     7861 ctggctgatc gtcgccacgc tggcggcgct gacgatcctg gtgaccgtgc ggctgtggat
     7921 ggtggaaggg ctcgtcctgc ggccggtgtc gatcgacggc ccgtcgatgg cgccggcctt
     7981 ggtgggattg cattacgccg tgacttgcga cgactgtgcc tttgcgttcg cgtgcgatgc
     8041 ccaaaatgcg ccggccagcg ggcaggt
//
LOCUS       DASUGB010001074         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1092184, whole genome shotgun sequence.
ACCESSION   DASUGB010001074 DASUGB010000000
VERSION     DASUGB010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092184"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFV87_20360"
     CDS             complement(<1..1273)
                     /locus_tag="VFV87_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106189.1"
                     /translation="MRSFHARSVASALILASLASTALAQDAGIPGLLPLPPAQPVYPV
                     YPVAQTAATDVLWSDQEVSPAPVVDAGQAEALPGTSVISPDYEQAMYGGCGAGTCGEC
                     NCCPNYYVYANTLWMTREKRGGFVTSIDSVTLDPRLFFCAPGFGDRWYGGFEVGGGWI
                     LGGCNNCGCGSCGGCCGTTALEVVYWGLFPSTAEQNPTDALISLIDFSDLDYNGATLD
                     TLFSPAEMHRVQFRYDINSVELQLVGNGGNGGPFGGGRTMGPGASRFGFGWMAGIRYI
                     DYSENFLFSADNDDTTFSNDPDEAHYQVDLSNDLIGFQVGTGVNYWVSQRFSTYAIGR
                     VGIYNNHVSMFQQIYGTAGPVTINNGPNTGEIYSVDSSKDELAIVGQLDFGARWNINR
                     SWSLDAGYRIVGLSGVAISEDNVQQSNFQNLD"
     gene            complement(2277..>2971)
                     /locus_tag="VFV87_20365"
     CDS             complement(2277..>2971)
                     /locus_tag="VFV87_20365"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX5106190.1"
                     /translation="AWVQQVNECVLSLDEGVGQLIQALKETGQLANTLVVFTADQGFS
                     MGEHGFRTKIAPYDANYASPLIVSQPGTIPEGKHCPQCVTAPDLIATFHARTGVAQPW
                     KMHGRDISPLLSDPEKAAWPHLCFYEHMGHHYGSDVTKELKAGGKEANHSNVPWYVVV
                     RDGRYKYIRYLQAGNMDELYDLKSDPEELTNLAGSAEHAKRVADLGNKLTAELRRTGA
                     DFIDLIPEPKAN"
BASE COUNT          545 a          915 c          966 g          545 t
ORIGIN      
        1 cgtccagatt ctggaagttg ctttgctgca cgttgtcttc ggaaatggcc acgccggaca
       61 ggccgacgat gcggtagccg gcatcgagcg accagctccg gttgatgttc caacgggcgc
      121 cgaaatcgag ctggccgacg atggccagct catccttcga gctgtccacg ctgtagatct
      181 cgccggtatt agggccgtta ttgatggtga ccggaccggc ggtgccataa atctgctgga
      241 acatgctgac gtggttgttg tagatgccga cgcggccgat cgcatacgtc gagaaccgct
      301 gcgacaccca gtagttgacc ccggtgccga cctgaaagcc gatgaggtcg ttgctcagat
      361 ccacctgata gtgggcttca tcgggatcgt tgctgaaggt cgtgtcgtcg ttgtcggcgc
      421 tgaacaggaa gttttcgctg taatcgatgt agcgaatgcc tgccatccag ccgaagccga
      481 accgcgaggc gccggggccc atcgtgcggc cgccgccgaa cggcccgccg tttccgccat
      541 tgccaacaag ctgcagttcg acgctgttga tgtcgtaccg gaattgcacc cggtgcattt
      601 ccgccgggct gaagagcgtg tctagcgtgg cgccgttata gtcgagatcc gaaaagtcga
      661 tcagcgaaat caaagcgtcg gtcggattct gttcggctgt gctggggaac agaccccaat
      721 acacgacttc caacgccgtg gtgccgcagc atccgccgca agagccgcac ccgcagttgt
      781 tgcagcctcc caggatccac ccgccgccga cctcgaatcc gccgtaccag cggtcgccga
      841 agcccggggc gcagaagaac agccgcgggt ccagcgtgac ggagtcgatg ctggtcacga
      901 acccgccgcg cttctcgcgg gtcatccaca gcgtgttggc atagacgtaa taattcgggc
      961 agcagttgca ctcgccgcag gttccggccc cgcagccgcc gtacatcgct tgttcgtagt
     1021 cagggctaat caccgaggtg cctggcagcg cctcggcctg gccggcatcg acgacgggag
     1081 caggagacac ctcctgatcg ctccacagca cgtccgtcgc cgcggtttgg gcgaccggat
     1141 aaacaggata gaccggctgg gccggcggca gtgggagcag gccgggaatg cccgcgtcct
     1201 gggccagggc ggtgctggcc aggctggcca ggatcagcgc cgaggcaacg cttcttgcgt
     1261 gaaaggacct catcgcacac tccttacggg ctcgatccat cgatttcgcg gcacgccgtc
     1321 cgttcgccgt gccgaggatg ggctcaaacg tccgtggtta agggttgggg cgtcgtgccc
     1381 ctgcaatggc gtccattcca cgctgcggct gtcatagcgg cagcaacacc cggcaatcca
     1441 tctaggtgtc agagggtgaa atcggcggcg cggcgacggc gatgtggccg attccgaggc
     1501 cgcgccgcga ttttgcgagc ggccgcgcgc cgcaatccgc acgcgcgctg caagcgctct
     1561 ggccggactt gcgggctgtg cgcggcatgc ctgccgcgcg ggcgcgcggg cagcgcgagc
     1621 ggaacgagcg atacaggcgg acagcgaaaa ccgcgcgcgg aagggacgac tcctgcgccg
     1681 cctatttgcc acgcaattgg cgattggcaa atcgactccg caaaaaccgc ggggtgcggt
     1741 cgcgccggag gcacgggact taggaccgca gggcggcctg tgggcgacgg cctatgcgga
     1801 tcgtgccgcg ctcacagcgc atgccgattg ctcgcgaagc gatcgcgagg cgcgatctgc
     1861 tgcgcagacc aggccgcggc gcgcggtagc aaaaagtcaa tcgcctgcga ttaccgcgga
     1921 tagcgcggag tcgcggatgg ggaaagagta gccacggacg gaacccagcg cggctccgcc
     1981 gcaaccgaat ggaaggtgag ttaatcaaag acgttcctcc cagatgtcgg aggacgctgc
     2041 ggtggctgtc caatccgcgg ggaatgctca attacctttc gtgcgtgagc gtgctcttga
     2101 aggacttcaa ggaatttgcc ttggactgca cgacgcggat tcgccgcggc agccagtgcc
     2161 gcccaactcc aacaactcgc cgcctgaaat cttctcgaca cacaaagata tggaacgttt
     2221 gtattacaga tgaaaggaat cgaagatccg tgttcgatcc acgttccatc cgtggctcaa
     2281 tttgccttgg gttctgggat caaatcgata aaatccgctc cggtccggcg caattccgcc
     2341 gtgagcttgt tgccgaggtc ggcgacgcgt ttggcgtgct ccgctgaacc ggcgaggttg
     2401 gtcagctcct cggggtcgga cttcaggtcg tacagctcgt ccatattccc ggcctgcagg
     2461 tagcggatgt acttgtaacg accatcgcgg acgacgacgt accacggcac attgctgtgg
     2521 ttggcctcct tgccgccggc tttcagttct ttggtcacgt cgctgccgta gtggtgtccc
     2581 atgtgctcat agaagcacag atgcggccag gcggctttct cgggatcaga caagaggggc
     2641 gagatatcgc ggccgtgcat cttccacggc tgtgcgaccc cagtccgggc gtggaacgtg
     2701 gcaatcagat ccggcgcagt gacgcactgc gggcagtgct tcccctcggg aatcgtcccc
     2761 ggctggctga cgatcagcgg cgaggcatag ttggcgtcgt acggcgcgat cttcgtccgg
     2821 aacccgtgct cgcccatcga aaacccctga tcagcagtga atacgacgag cgtgttggcg
     2881 agctggcccg tttccttcag cgcctggatg agctggccga ccccttcgtc gagcgacagg
     2941 acgcactcgt tgacctgctg gacccaggcg g
//
LOCUS       DASUGB010001075         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1370051, whole genome shotgun sequence.
ACCESSION   DASUGB010001075 DASUGB010000000
VERSION     DASUGB010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370051"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFV87_20370"
     CDS             <1..765
                     /locus_tag="VFV87_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106191.1"
                     /translation="GKPRELAITQNEIDELLITVSPKRLAELDPSQPQLYREYAEELA
                     EKHRDPEARDAAIRLYHLAAVLGEGPLRRGSLLGLAALARSPEEERKFRAAAYLYDPE
                     HDARVLAAARDSIVTTAPGASQALAELAAALRLIRQGKGREAKALLDRPLVREQAGGL
                     EKIITLEGLLALSESRDLSDEQLSKLLQAELAIDRSLGAGGSDLTSSSPSLQLASWSG
                     AVKSHGLAPLPSLALDSLTEFDPRECLYRDGRWVRP"
     gene            complement(772..2001)
                     /locus_tag="VFV87_20375"
     CDS             complement(772..2001)
                     /locus_tag="VFV87_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5106192.1"
                     /translation="MKCDYDVVVIGAGAAGLVAAAESCQRGRRTLLIEKNRRPGVKIL
                     MSGGTRCNITHATDKWGIVRAYGEQGSFLHSALAALSPADLVQLIEAEGVPTKVEESG
                     KIFPVSNRATDVLEALLLRLQRSGAELALAEPVRGLERLAEGFRVQTAARELAAHKLV
                     ITVGGQSYPGSGTTGDGYAWAGALGHTVVPPRPALVPLTTGEAWIRALSGVSVPDASL
                     TLDLQTKPQPAARGSLLFTHFGLSGPVVLDISRYVTALPDPAAAMLLVDFLPGQKLEQ
                     LDEALRSTAASDGKRQVAGILAEHMPRRLIDALLSRASLPLDLRGAQISKTARAALVA
                     ALKRTAIPITGTRGFAKAEVTAGGVSLAEIDSRTMESKLVPGLYFAGEILDLDGPIGG
                     YNFQAAFSTGFLAGKRA"
     gene            complement(2090..2293)
                     /locus_tag="VFV87_20380"
     CDS             complement(2090..2293)
                     /locus_tag="VFV87_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106193.1"
                     /translation="MFFLATSLLAPQLWYLVPLIVSVSLVYGATRHELPRPILQHAWH
                     TAVWMLVFIGAIFAVLWLVSLWL"
     gene            complement(2323..>3021)
                     /locus_tag="VFV87_20385"
     CDS             complement(2323..>3021)
                     /locus_tag="VFV87_20385"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEX5106194.1"
                     /translation="NPRSPSALRNAYVPWCLTPDPGTALAPPAASARIALATIHHRPR
                     LCLMPSPAKLPAETTLQIDLRNPNTAALLAWLWPGAGHLYQRRYAKAVLFMVCILGTY
                     FFGLALGEGKVVYAAWNQSDRRWQYPLQLGVGLPAMPALVQSMIVRRGGEPILGGIMR
                     PPEPGFHPGDPGELAQWHHDLNLRFELGTLYTMVAGLLNVLAIWDAYAGPVISEPGKE
                     RDPPEDGNDENEKA"
BASE COUNT          548 a         1015 c          962 g          496 t
ORIGIN      
        1 ggcaagccgc gtgaattggc cattacccag aacgagatcg acgagttgct gatcaccgtc
       61 tctcccaaac ggctggccga actcgaccct tcgcagccgc aactttaccg cgaatacgcc
      121 gaagaactcg ccgagaaaca ccgcgacccc gaagcccgcg acgccgcgat tcgcctctac
      181 cacctggcgg ccgtgctggg cgaggggccg ctccgccgcg gctcgctcct gggtctggcg
      241 gccctcgccc gctcgcccga ggaagagcgg aagttccgcg ccgcggccta tttatacgac
      301 cccgagcacg acgcccgagt gttggctgct gcccgcgact ccattgtcac caccgcaccc
      361 ggcgcctccc aggccctggc cgagttggca gccgccctgc gactcatccg ccaaggcaaa
      421 ggccgagagg ccaaagccct tctcgacagg ccgctcgtcc gcgagcaggc cggcggcctg
      481 gaaaaaatca ttacgctaga gggcctcctc gccctgagcg aatcgcgcga cctctccgac
      541 gagcagctat ccaaactcct gcaagccgaa ctggcgatcg accgctccct cggcgccggc
      601 ggttcggatt taacctccag cagtccctcc ctgcagctgg caagctggtc cggcgccgtc
      661 aaatcgcacg gtctcgcccc gctcccctcg ctcgctctcg attcgctcac cgaattcgac
      721 ccccgcgaat gtctctatcg cgacggcagg tgggtccggc cgtaactgca actacgcgcg
      781 ctttccagcc aggaaacccg tgctgaaggc agcctgaaag ttgtacccgc cgatcgggcc
      841 gtccaggtcg agaatctcgc ccgcaaaata caggccgggg accagcttgc tctccatcgt
      901 gcggctgtcg atttcggcca ggctgacgcc gccggcggtg acttccgcct tggcgaagcc
      961 gcgcgtgccg gtgatcggaa tggccgtgcg tttcagtgca gcaacgaggg cggcgcgtgc
     1021 ggtcttcgag atttgtgccc cgcgcaggtc gagcggcagg ctcgcgcgcg acaggagggc
     1081 gtcgatgagc cgccgcggca tatgttcggc cagtatcccc gccacctgac gcttgccgtc
     1141 gctggccgcg gtgctgcgca gcgcttcgtc gagctgctca agcttctggc caggcagaaa
     1201 atcgaccagc agcatcgcgg cagcaggatc gggcagcgcg gtgacatagc gactaatgtc
     1261 gaggaccaca ggcccggaga gcccgaagtg ggtaaagagc aatgagccgc gggccgccgg
     1321 ctgcggcttt gtttgcaggt cgagtgtcag cgatgcgtcc gggactgaca cgccgctcag
     1381 cgcgcggatc caggcctcgc cggtcgtcag aggcacgagg gccggccgcg ggggcacgac
     1441 cgtgtggccg agcgccccgg cccaagcgta accgtcgcca gtcgtgccgc tgccgggata
     1501 cgactggccg cccacggtga tcacgagctt gtgcgcggca agctcccgcg ccgcggtctg
     1561 cacgcgaaat ccttccgcca gcctctccaa tccacgcacc ggctcggcaa gagccagttc
     1621 cgcgccgctg cgctgcagcc gcagcagcag ggcctccagg acatccgtgg cccggttgct
     1681 caccgggaaa atcttgcccg actcctcgac tttcgtcggg acgccttcgg cttcaatcaa
     1741 ttgcaccaaa tcagccggcg acaaggccgc cagcgccgag tgcaggaagc tgccttgctc
     1801 gccataggcc cggacgattc cccatttgtc ggtcgcatgt gtgatgttgc agcgtgttcc
     1861 tcccgacatc aggatcttga cgccggggcg tcggtttttc tcaatcaaga gagttcgccg
     1921 gccgcgctgg cacgattccg ctgcggcgac cagcccggcg gcacccgcgc cgatcacgac
     1981 gacgtcgtag tcacatttca aagttcaatg ttccaggttt catcgcgcaa agcggatgca
     2041 gcaccagccg ctttgaatct tgcactttga acctggagtc gcgtcctgtc tagagccaca
     2101 gcgaaaccag ccacagcaca gcgaaaatcg ccccgatgaa cacgagcatc cagacggcag
     2161 tgtgccaggc atgctgcagt atgggccgcg gcaactcgtg ccgtgtcgcc ccatagacca
     2221 ggctgaccga aacgatcagc ggcacgaggt accaaagctg gggggccagg agggaagtgg
     2281 cgaggaaaaa catggctgtt tggtccatac acggcttgca agtcacgcct tctcgttttc
     2341 gtcgttgccg tcctccggcg ggtcgcgttc cttgccgggc tcgctgatca cggggccggc
     2401 ataggcgtcc cagatcgcca ggacgttcaa gaggccagcg accatcgtgt acaacgttcc
     2461 cagctcgaac cgcaggttca ggtcgtggtg ccattgggcc agttcgcccg gatcgcccgg
     2521 atggaatccg ggttccggcg gacgcatgat tccacccagg atcggctcgc ctccgcggcg
     2581 gacaatcatg ctctgcacca gggccggcat ggcaggcagg ccgaccccaa gctgcagcgg
     2641 gtattgccag cggcggtcgg actgattcca agcggcatag acgaccttcc cctcaccgag
     2701 ggccaggccg aagaaatatg tccccaatat gcagaccata aacagcacgg ctttggcgta
     2761 gcggcgctgg tagagatggc cggcgccggg ccagagccag gcgaggaggg ccgccgtatt
     2821 gggattccgg aggtcgatct gcagcgtcgt ttcggccggg agtttggcgg gagagggcat
     2881 gagacataac cgcgggcgat gatggatcgt ggccagggct attctagccg aggcagcggg
     2941 tggggccaga gcagttccgg ggtcaggagt caggcaccac gggacatagg catttcgcag
     3001 ggcggaaggc gatcttggat t
//
LOCUS       DASUGB010001076         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1924891, whole genome shotgun sequence.
ACCESSION   DASUGB010001076 DASUGB010000000
VERSION     DASUGB010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924891"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFV87_20390"
     CDS             <1..273
                     /locus_tag="VFV87_20390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5106195.1"
                     /translation="NYEAYKDRGFDVVGISIDQDRPALEEFLGERKLPWTVLHDKEHE
                     GQHPATVEYGIFYIPCVILVDKEGKVISTRARGEELGRLLKDLLGE"
     gene            complement(283..2169)
                     /locus_tag="VFV87_20395"
     CDS             complement(283..2169)
                     /locus_tag="VFV87_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5106196.1"
                     /translation="MHHLDLILTLTGGLAAALILGYITQRLGLSPIIGYLLAGVAVGP
                     TTPGFIANPALANEMAELGVVLLMFGVGLHFHLKDLLAVRHVAVPGAIGQSLTATLLG
                     CLLTMAFGWDLKAGIVFGLAISVASTVVLLRVLADNNDLHTPAGHIAVGWLVVEDVFT
                     VLVLVMLPVLFQPAGPTANAAAADGMGAGGGAGSVALALGWSVLKIAIFVGFIFFVGG
                     RVIPWLLEQVAATRSRELFTLAVLVLALGIAVGAAKLFGVSMALGAFLAGMVVGRSDY
                     SSRAASEALPMRDAFAVLFFVSVGMLFKPMYLLEAPILVAATVGTVLVGKPLAALVIV
                     LLLKYPLRVALAVSVALAQIGEFSFILATLGTELHILPAAAMNTLVAAAIVSISINPA
                     LYRLIDLMESRAKRSPRLWRLLSGRSSEALQASATAHLQEPQGQVIVVGYGPVGRTLV
                     RLLLENDIPPTVVELNMDTVRRLRDEGLRAVYGDAAHKETLIAAGIEQAAALLLTSSA
                     VHGADEAIRVARECNPAIRVLARAGYLREVPTLRRAGADAVFSGEGEVALTMTEFMLR
                     QLGATAEQIDRARDRIRNELFGTPQTVELLLPLPHQAAGAPQEPAGAEHQTAGPEPAH
                     SPAE"
BASE COUNT          499 a          864 c          814 g          366 t
ORIGIN      
        1 aactatgagg cctacaagga ccgcggcttc gacgtggtgg ggataagcat cgaccaggac
       61 cggcccgcac tggaagagtt tctgggcgag cggaagctgc cctggaccgt gctacacgac
      121 aaggagcacg agggccagca cccggcgaca gtggaatacg gcatcttcta cattccctgc
      181 gtgatcctgg tggacaagga ggggaaggtc atttcgaccc gcgcccgcgg cgaagagttg
      241 ggccggctgc tgaaggacct gctgggggag tgaccgccgg gctcactcgg cgggcgaatg
      301 tgcgggctct ggcccggcgg tctgatgctc cgctccagcg ggctcctggg gcgctccagc
      361 ggcctggtgc ggcagcggca ggagcagctc gaccgtctgc ggcgtgccaa acagctcgtt
      421 gcgaatgcgg tcgcgggccc ggtcgatctg ctcggccgtg gccccgagct ggcggagcat
      481 gaactcggtc atcgtcaggg cgacttcccc ctcgcccgag aacaccgcgt cggctcccgc
      541 ccgccgcaag gtcggcacct cgcgcaaata tcccgcccga gccagtacgc gaatcgccgg
      601 gttgcactcg cgggcgacgc ggatcgcctc gtccgctccg tggacggccg acgatgtcag
      661 caacaacgcg gctgcctgct cgatcccggc agcgatcagc gtctccttgt gggccgcatc
      721 gccatacacg gccctcagtc cctcgtcgcg caggcggcgg accgtatcca tgttcaactc
      781 gacgaccgtt ggcggaatgt cgttttccag cagcaaccgc acgagcgtcc tcccgaccgg
      841 tccgtaaccg acgacaatca cctgcccctg cggctcttgc aagtgggccg tggcgctagc
      901 ttgtagagcc tcggatgaac gcccgctcaa gagccgccag aggcgcggag aacgcttcgc
      961 gcgggattcc atcaggtcga tcagccgata gagagccggg ttgatgctga tcgagacgat
     1021 ggcggcggcc acgagcgtgt tcatcgccgc cgccggcagg atatgcagct cggtgccgag
     1081 cgtcgccagg atgaacgaga attcgccgat ctgggccaat gccaccgaca cggccagcgc
     1141 cactcggagc gggtacttga gcaagagcac gatcacgagg gcggccagcg gcttgccgac
     1201 aagtacggtg ccgaccgttg cggcgacgag aatcggagct tcgagcagat acatcggctt
     1261 gaacagcatg ccgactgaaa cgaagaatag cacggcaaag gcgtcgcgca tgggcagggc
     1321 ttcggaagcc gcccgcgagc tgtaatcgga ccggcccacg accatgccgg ccaggaacgc
     1381 cccaagggcc atcgagacgc cgaacagctt ggccgcaccc accgcgattc ccagcgccag
     1441 aacgagcacc gccagcgtga acagctcgcg cgagcgagtg gcggccactt gttccagcag
     1501 ccacggaatc acgcgcccgc cgacgaagaa aataaaaccg acgaagatgg cgattttcag
     1561 caccgaccat cccagcgcca aggccacgct gcctgctccg ccgcctgccc ccatcccatc
     1621 cgccgcagcg gcatttgctg ttggccctgc aggctgaaag agcaccggca gcatgaccag
     1681 caccagcacg gtgaagacat cctctacgac cagccagccg acggcgatgt ggccggcggg
     1741 cgtgtgcagg tcattgttgt cggccagcac gcggagcagg accaccgtgc tggccaccga
     1801 gatcgccagg ccgaatacga ttcccgcctt caggtcccag ccaaaagcca tcgtcagcag
     1861 gcagcccagg agcgtcgcag tcaggctctg gccgatcgct ccaggcaccg cgacgtggcg
     1921 gacggccagc aaatccttga ggtggaaatg caggccgacg ccgaacatca agagcacgac
     1981 cccaagctcg gccatctcgt tggccagggc cgggttggcg atgaatccgg gtgttgtcgg
     2041 ccccacagcc acgccggcca gcaggtagcc gatgatcggc gagagcccca gccgctgggt
     2101 gatatagccc aggatcaagg ccgcggccag gccgccggtg agtgtcagga tgaggtccaa
     2161 atggtgcatg ccccgatggt agcgggacgc tcgccgggcg caaacccgaa tccacgtcga
     2221 tcgggcccaa tccctattgg tcggcgaacc ttcgtcatcg taagcgacaa aaagatgtgc
     2281 tcccctctga cctccgatct ccgatctccg acctcgcgtc ggcagaaaaa ccttgacctg
     2341 cggcgccggc gagcgtccaa tcgaaagatg agcatgacac gctgctgccg cgccgccgca
     2401 tcccggaact tgccgccatg gatacccact atgcagtgct cggcataagc ccgcgcgcca
     2461 cgcaggagga aatccagcgg gcctattggc acctggcggc tcacgttcat cccgacctga
     2521 acccggacca gctcgccgcc agc
//
LOCUS       DASUGB010001077         3633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_675261, whole genome shotgun sequence.
ACCESSION   DASUGB010001077 DASUGB010000000
VERSION     DASUGB010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675261"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFV87_20400"
     CDS             complement(<1..200)
                     /locus_tag="VFV87_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5106197.1"
                     /translation="MLDKRHLYRDLAALALVGVVIFLALALATYDPADPVGVAVAPFD
                     RLFQPDPLVYPPREEVRNICGH"
     gene            704..2203
                     /locus_tag="VFV87_20405"
     CDS             704..2203
                     /locus_tag="VFV87_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX5106198.1"
                     /translation="MDFQTYETDGFYDELLTPDHQARPRGEVLVKRLAALPTEDLQRK
                     QKAAELAMLNMGITFNVYGHEAGTEKVWPFDIIPRIIDGQEWDYIERGLKQRITALNL
                     FIDDMYHGRRIIADGAVPEWLIASAKNYLKQCQGMNPSKGIWCHITGTDLVRDGDGQV
                     YVLEDNLRCPSGVSYVLENREIMKRTFPQSFDGFSILPVENYPDKLLEMLTHIAPDGV
                     KEPNVVVLTPGIYNSAYFEHCFLARQMGVELVQGSDLVCSGGYVYMRTTTGLERVDVI
                     YRRIDDEFLDPHTFRSDSTLGVPGLMDVYRAGRVALANAPGTGVADDKAVYAFVPQMI
                     KYYLGEDIILPNVPSFVCGEPQQCEHVLANVDKLVIKPANESGGYGILLGPKASHQER
                     ERYKELIQSNPRNYIAQPMLALSRVPTIIEDHFEGRHVDLRPYILYGKEIFVLPGGLT
                     RVALRKGSLVVNSSQGGGSKDTWVLRGSHLHNGRSEVQSPKSKVQGQFA"
     gene            2255..3238
                     /locus_tag="VFV87_20410"
     CDS             2255..3238
                     /locus_tag="VFV87_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX5106199.1"
                     /translation="MLSRVADSIFWMSRYIERAANVARFIHVNLKLAIDLGPTTGGQW
                     ASLVYTTGDQDDFAERYGDPDQRKVIEFLAFDQANPNSIVSCLRQARENARTVREMIS
                     SLMWEELNKFFLTVRSAANEHGAVENPFDFFQQVILTGHLLSGINDGTMSHGEPWHFT
                     RLGRFLERADKTSRILDVKYYILLPSVADVGTAVDTIQWSALLESASALEMYRKRHGR
                     ITPDKVADFLILDREFPRAMHYCLIEAEKSLASITGNPGGDRIYTPAEQQLGRLRSDL
                     NYSRIDEIIKLGLHEFIDQFQDRLNEVGEAIHETFFAPHPVPAPATQSQMQ"
     gene            3373..>3633
                     /locus_tag="VFV87_20415"
     CDS             3373..>3633
                     /locus_tag="VFV87_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106200.1"
                     /translation="MNLALSASPPPPVSPLLADYRARDGAWDEMRDGGAVRAAWQPLV
                     HSLADLDRAALAGRWEQARRLIHENGVTYNVHGDPEGLARPWE"
BASE COUNT          709 a         1142 c         1125 g          657 t
ORIGIN      
        1 cagtggccgc agatgttccg cacctcttcg cgcggcggat acaccagcgg atcgggctga
       61 aacagccggt cgaagggtgc gaccgccacc cccacgggat cagccgggtc gtacgtcgcc
      121 agcgccagcg cgagaaaaat caccacgccc acgagggcga gcgcggctag atcacggtag
      181 agatgtcgtt tgtcgagcac gggcgcgtcg gggaacgtgg aaaccggcgg tgggccggca
      241 ggtccaaggg cgcgtccgtg cggaagacaa tccgtggtgt ctctggttag atcggcggaa
      301 cttccctgtc gcccggagct tttgctaagt gcgtcgtcca gcttgcctga ccggcagcgt
      361 ttcgttgccg agccacgaga tgggccattt cagaccgccg aagtgtagca cgcaggatcg
      421 agtgacctgg tcaaattgac cctcgggttt gactcgaatg cgctgcgaac gtagtagacg
      481 aatcgattgt gcggtctagg gaagcaacga aagcaggatg ggtcattctg cgactggtgg
      541 acgatgcttt aagatgtgcg gtctgggcgg catggcacat gccttaaccc gacaatcgcc
      601 ccgaacggcc cttttgggag ccggaatgct cagcctgcgc cccaggcgtg cctgattctt
      661 gggcagcaat cggcacgccg gactcacgaa atggagtccg agtatggatt ttcagactta
      721 cgaaaccgac ggcttctacg acgagctgct gactcccgat caccaggccc gtccgcgggg
      781 ggaagtgctg gtcaagcggc tggcggcgct gccgaccgaa gacctgcagc gcaagcaaaa
      841 ggcggccgag ctggcgatgc tcaacatggg catcacgttc aacgtctacg gtcacgaggc
      901 gggcacagaa aaagtctggc cgttcgacat cattccgcgc atcatcgacg ggcaggaatg
      961 ggactacatc gagcgcggcc tgaagcagcg aatcaccgcg cttaacttgt tcatcgacga
     1021 catgtaccac ggccgccgca tcattgcgga cggcgccgtg ccggagtggc tgattgcttc
     1081 ggccaagaat tacctcaagc aatgccaggg aatgaacccc agcaagggaa tctggtgcca
     1141 catcacgggc accgacctgg tgcgcgacgg cgacgggcag gtgtacgtgc tggaagacaa
     1201 cctccgctgc ccttccggcg tgtcgtacgt gctcgaaaac cgcgagatca tgaaacggac
     1261 atttccgcag agcttcgatg gcttctcgat cctgccggtc gagaactatc ccgacaagct
     1321 gctggaaatg ctcacgcaca tcgcgccgga cggcgtgaag gaaccaaacg tcgtcgtcct
     1381 cacgccgggc atttataact cggcttattt cgagcactgc ttcctggctc ggcagatggg
     1441 cgtcgagttg gtgcaaggct cggacctcgt ctgctccggc ggctacgttt atatgcggac
     1501 cacgaccggc ctggagcgag tcgatgtcat ttaccgccgg atcgacgacg aattcctcga
     1561 cccgcacacg ttccgctccg attcgacgct cggcgtcccc ggtttgatgg atgtctatcg
     1621 cgccggccgc gttgccctgg cgaatgcccc cggcacgggc gtggccgacg acaaagcggt
     1681 gtatgcgttc gtgccgcaga tgatcaagta ctatctgggc gaggacatca tcctgccgaa
     1741 cgtgccgtcc tttgtctgcg gcgagccgca gcagtgcgag catgtgctgg cgaacgtcga
     1801 taagctcgtc atcaaaccgg cgaatgaatc gggcgggtac ggcatcctgc tcgggccgaa
     1861 ggcttcgcac caagagcgcg agcgctacaa agaactcatc caatcgaatc cgcggaacta
     1921 tattgcccag cctatgctgg cactgtcgcg cgtgccgacg atcatcgagg atcatttcga
     1981 ggggcggcat gtcgatctgc ggccgtatat tctgtacggc aaggagattt tcgtgctccc
     2041 cggcgggctc acgcgcgtcg cgctccgcaa aggctcgctg gtcgtcaact cctcgcaagg
     2101 cggcggcagc aaagacacct gggtcttgcg gggcagccac ctgcacaacg gccgatcaga
     2161 agtccaaagt ccaaagtcca aggtccaagg gcaattcgca tgagacgcct gccttcaact
     2221 ttgaactttg ggcattggac cttggactag ctcgatgctc agccgcgttg ccgattcgat
     2281 cttctggatg agccggtaca tcgagcgcgc ggcgaacgtg gcccggttca ttcacgtcaa
     2341 cctgaagctg gcgatcgatc tggggccgac aaccggcggc cagtgggctt cgctcgtcta
     2401 taccaccggc gaccaggacg actttgccga gcggtacggc gatcccgatc agcggaaggt
     2461 catcgagttt ctggccttcg accaggccaa tcccaattcc atcgtctcct gcctgcggca
     2521 agcgcgcgag aacgcccgca ccgtgcggga gatgatctcg tcgctgatgt gggaagagct
     2581 gaacaagttc ttcctcaccg tccgctccgc ggccaacgag cacggcgccg tggaaaaccc
     2641 gttcgacttc tttcagcaag tcattctcac ggggcatttg ctgagcggaa tcaacgacgg
     2701 caccatgtcg cacggcgagc cgtggcactt cacgcggctg ggccgcttcc tggagcgggc
     2761 ggacaagacc tcccgcatcc tcgacgtgaa gtactacatc ctgttgccga gcgtcgccga
     2821 cgtggggacc gccgtcgata cgatccagtg gtcggccttg ctcgaatcgg ccagcgccct
     2881 ggaaatgtat cgcaagcgcc acgggcggat tactcccgac aaggttgccg acttcctgat
     2941 cctcgaccgc gagttcccgc gggccatgca ctattgcctg atcgaggccg aaaaatcgct
     3001 ggcgtcgatc accggcaatc ccggcggcga ccggatttac acacccgccg agcagcagct
     3061 cggccgcctg cgatccgact tgaactactc gcggatcgac gagatcatca agctgggact
     3121 gcacgagttc attgaccagt tccaggaccg actgaacgag gttggcgaag caattcacga
     3181 gacgtttttt gccccgcacc ccgtgccggc tccagcgact caatcgcaaa tgcagtaggg
     3241 tgggaccagc tcgcgaagcg agcacaggcc caccactgcg cttgtcgttt ttgactggca
     3301 cagattatgc tggcagtggg cgaatgacgc tcgccttccg atccatcgct cacggacgag
     3361 tgctcccgac cgatgaacct ggccctctcc gcttcgcctc ccccgccggt cagcccgctc
     3421 ttggccgact atcgcgcacg ggacggcgcc tgggatgaaa tgcgcgacgg cggcgccgtg
     3481 cgggccgcct ggcaaccgct ggtccactcg ctggccgacc ttgaccgcgc ggccctggcc
     3541 ggccgctggg agcaggcccg ccggctgatt cacgagaacg gcgtcacgta caacgtccac
     3601 ggcgatccgg aagggctggc ccgcccgtgg gag
//
LOCUS       DASUGB010001078         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_397776, whole genome shotgun sequence.
ACCESSION   DASUGB010001078 DASUGB010000000
VERSION     DASUGB010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397776"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV87_20420"
     CDS             <1..261
                     /locus_tag="VFV87_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106201.1"
                     /translation="NDDAGRDHWASVFSGFFAGGGIKAGTVVGSSDEDGAQPKDRPVR
                     VADIHATICHALGIDAKKEVMTPLRRPMKLVDGGEVVTEWLA"
     gene            531..2924
                     /locus_tag="VFV87_20425"
     CDS             531..2924
                     /locus_tag="VFV87_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106202.1"
                     /translation="MRHLLPLVALVSVSVVFPLIAAAQQPEAAPADDPAAGAVRGSIV
                     EDRAAKKLLEAGDARLEADEGTKALEIWKSVIERYPRSKYRFDAHLRLGNYYLDQERA
                     YDRARVHFEAAAAEDNKDEEQRAEGTLKLGVCFYHARNFGKCFQVLRDVIEKFPVSPQ
                     VNEAYYYIGLGHFQQGHYSRAIQALEKVGTTLSGENTQGEKLEAGKRLFVKIEDADLA
                     VLEADQTVKVIARSSGGDEETFDCFPVGRNVRLVLGSIVSKLGRPQPGNGSLEVKGGD
                     KVEVRYTDEHTAEKKLKVPVLKEVTVVGNAMVAITDGAFAETVNGVVLGKTANLRVND
                     PDRDTSDAADTIQAIAEVYRLKTDEELEAEAIARAKEAPATPAAPPPANPNDPNARPA
                     EEILPDEPEIERWKLIDRVEVTLTEVNPQPIVSDRKVVGQASSLPENESASSQGPGAS
                     DQPADDGTIHSGVFHFSVPLVKIETPVAGDPILQAMPADEVRLTYLDEIHTWDGVKQV
                     TAAARCLEGNIGGVRVTKAEISDQELKIQTQLKTADALTQIGNRYKEFGLKDKANEKY
                     NQALTVCEEVADDARKLGGSLLESLYVELWNIYFAMDRLELAAAMCQRLQSEFPNSGF
                     VDDALLQLADVAKKQGDLQRAIGIYTRLVGMPSSQLRGEAQFGVAECYEAMASKQEGA
                     AAAQLQDRAFQEFKRVYDEFSDSGRVGEAVAKMANYYYVQKDYARATDTFETVLNNHP
                     DARFLDVILFNYGRCLYRMERRAEAKQRFDQLISEFPESPLATDAKKISDALAKSGM"
BASE COUNT          601 a          966 c          930 g          435 t
ORIGIN      
        1 aacgacgacg ccggccgcga ccactgggcc agcgtcttca gcggcttctt cgccggcggc
       61 ggtatcaagg ccggcacggt cgtcggctcg tcggacgaag acggcgccca gcccaaggac
      121 cgcccggtcc gcgtcgccga catccacgcc acgatctgcc acgccctggg aatcgacgcc
      181 aagaaggaag ttatgacacc gctgcggcgc ccgatgaagc tcgtcgatgg cggcgaggta
      241 gtcaccgaat ggctggcgta gccagccgtg gggcaggctt ccagcctgcc gcgaaggatg
      301 tggcgtcacg ctcggtccca cgctccgcgt gggaccgcac gcctcgccgc tccgcggccg
      361 gtggcggacg acgcagagcg tctgtgcgga cgttccacgc ggagcgtggg aacgagcgta
      421 ggcgacaata tgaattgccg gacccgcccc gtccacttac cgtggaaggc ttgtccggca
      481 ggccaggatg gccggacttc ccctgaacct ccgccttcca ggcccacccg atgcgtcacc
      541 tgctgccgct tgtcgcactc gtttccgttt ccgtcgtttt tccgctgatc gccgccgctc
      601 agcagcccga ggccgcgccg gccgacgacc ccgccgccgg cgccgtccgc ggctccatcg
      661 tcgaggaccg cgccgccaaa aagctcctcg aagccggcga tgcccgcctc gaagccgatg
      721 aggggacgaa agcgctcgaa atctggaagt ccgtcatcga gcgctatccg cgtagcaagt
      781 accgcttcga cgcccacctg cggctgggca actattacct tgaccaggaa cgggcctacg
      841 accgggcccg cgtccatttc gaagccgccg ccgcggagga caacaaggac gaggagcagc
      901 gggccgaggg aacgctcaag ctcggcgtct gcttctatca cgcccgcaac ttcggcaagt
      961 gctttcaggt gctgcgcgac gtgatcgaaa agttcccggt cagcccgcag gtcaacgaag
     1021 cgtattacta catcggcctc ggccacttcc agcaaggcca ctacagccgg gcgatccagg
     1081 ccctggagaa agtcggcacg actctctcgg gcgagaacac gcagggcgaa aagctcgagg
     1141 ccggcaagcg cctgttcgtg aaaattgagg acgccgacct ggccgtgctg gaggccgacc
     1201 agacggtcaa ggtgatcgcc cgttcgtccg gcggcgatga ggagacgttc gattgcttcc
     1261 cagtcggccg caacgtacgg ctggtgctgg gctcgatcgt ctcgaagctc ggccgcccgc
     1321 aaccgggcaa tggctcgctg gaggtgaagg ggggcgacaa ggtcgaggtc cgctacaccg
     1381 acgaacacac ggccgagaaa aagctgaagg tgccggtgct caaggaagtt accgtcgtcg
     1441 gcaatgcgat ggtggcaatc accgacggcg cgtttgccga gacggtcaac ggcgtggtcc
     1501 tcggcaagac cgccaacctg cgggtgaacg atcccgaccg cgacaccagc gacgcagccg
     1561 atacgatcca ggccattgcc gaggtctatc gcctcaagac tgacgaagag ctcgaagccg
     1621 aagcgatcgc ccgcgcgaaa gaagcgccgg caacgcccgc cgccccgccg ccggccaatc
     1681 cgaacgatcc gaatgccagg ccagccgaag aaattctgcc cgacgaaccc gaaatcgagc
     1741 gctggaagct gatcgaccgc gtggaagtga cgctcaccga agtcaacccg cagccgatcg
     1801 tgagcgaccg taaggtcgtg gggcaggctt ccagcctgcc agagaacgag tcagccagta
     1861 gccaggggcc aggagccagt gaccagccgg cagatgatgg cacgatccac tccggcgtgt
     1921 tccacttctc cgtcccgctc gtcaagatcg aaacgcccgt cgccggcgac ccgatcctgc
     1981 aggccatgcc ggccgacgaa gtgcggctga cgtatctgga tgaaatccac acgtgggacg
     2041 gcgtcaagca agtcacggca gcagcccgct gtctggaagg caacattggc ggagtccgcg
     2101 tcacgaaggc ggagatttcc gaccaggagc tgaagatcca gacgcagctc aagacggctg
     2161 acgccctgac tcagatcggc aatcgctaca aggagttcgg cctcaaggac aaggccaacg
     2221 agaagtacaa ccaggcgctg accgtctgcg aggaagtcgc cgacgacgcc agaaaacttg
     2281 gcggcagcct cctggaatcg ctctacgtcg agttatggaa catctatttt gcgatggatc
     2341 gcctggaact ggccgccgcc atgtgccagc gattgcagag cgagttcccc aacagcggct
     2401 ttgtcgatga tgccctgctg caactggccg acgtggccaa gaagcagggc gacttgcagc
     2461 gggcgattgg catctacacg cgtttggtcg gcatgcccag cagccagctt cgcggcgagg
     2521 cccagttcgg cgtcgccgag tgctacgaag cgatggcatc caagcaggaa ggggccgcag
     2581 ccgcccaatt gcaggatcgg gcgttccagg agttcaagcg ggtctatgac gaattctccg
     2641 acagcggacg cgtcggcgag gcggtcgcca agatggccaa ctattactac gtgcaaaagg
     2701 actatgcccg ggcgaccgac acgttcgaga cggtcctcaa caaccacccc gatgcccgct
     2761 tccttgacgt aatcctgttc aactacggcc gctgcctcta tcgcatggag cggcgggccg
     2821 aagccaagca gcgcttcgac cagctcatca gcgagttccc cgaaagcccg ctggcgaccg
     2881 atgccaagaa gatcagcgac gcgctggcga agagtggcat gtaatcctgt ag
//
LOCUS       DASUGB010001079         7906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_260182, whole genome shotgun sequence.
ACCESSION   DASUGB010001079 DASUGB010000000
VERSION     DASUGB010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260182"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VFV87_20430"
     CDS             <1..1680
                     /locus_tag="VFV87_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106203.1"
                     /translation="PDRWWSYEGVDAIVLATSDEAFLDKLTSPQIDAIAQWVRLGGRL
                     ILCVGARGEKLLAEGGPWAALAPGKLADVSPLRDGTGLETFTASKLPHGEERYERDRP
                     LVSRLTDASGTVLVEEGGAISANRPLIVHAPRGLGQVTYVGIDLDHPALASWKGRPSL
                     IARLLQGKEPREQSDREANRSVTHLGYEDLVGQLRAALDQVPTVKIVNFTTVSILTIV
                     YLLLIGPGDFLLLSRLGWPRQVTWFTFPLVAIALGAVAWLFGSQSHGEKVRLNQAELV
                     DIDASSGVARGTVWAHLYSPATDHFDLQMQIEPPPTSSPGNAAQPQGWLAWQGLPGES
                     MGGLASRQVSLAAADPYSISLPRPQPTMENLPVQVASSKSLSGRWWAQVDVQAKNQLA
                     ENQYGVVTGTFEQPLPFELTDCLLIHGEKLYRLGKLPAGHTVHMDDFAPLNLEARLTE
                     RTVVLSKDVSTPWRKDLVDVPRILLMMMFHEAARGSSFTGLTNRYQPYVDLTEQVRLG
                     RAVLVGRTEKGACQIHRGDRPLADPSAVQTWTWYRVILPVGKEQPEQPTNP"
     gene            1739..2671
                     /locus_tag="VFV87_20435"
     CDS             1739..2671
                     /locus_tag="VFV87_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5106204.1"
                     /translation="MIKTHNLTKKYGESYAIQAIELDLAAGDLFGFIGPNGAGKTTTM
                     KILATLLNPTYGEAYVCGHSIYRDPKEIRRLVGYMPDFFGVYDDMKVIEYLEFFAAAY
                     RINGSKRRQVCNEKLDVVDLDFKRDAYANTLSRGQTQRLGLARVLLHEPQVLLLDEPL
                     SGLDPRARIEMRAVLRRLGQQGKTIMVSSHILPELADMCNKVGIIDRGVMTVNATVAD
                     VMKQVRERIVLHVQVTGDLDAAARLLEQNDLVESVAIVKEQIVVTLKKGVEDYSDLSP
                     LLISAGHKIKLFREEEVNLESAFMALTKGLGQRV"
     gene            complement(2690..2881)
                     /locus_tag="VFV87_20440"
     CDS             complement(2690..2881)
                     /locus_tag="VFV87_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106205.1"
                     /translation="MIQFSLTQGERDLLVEILQESLENRRVEVRRTEFSTEFRRHLQN
                     QEQLVRGMLQKLQLAPAAT"
     gene            3049..4455
                     /locus_tag="VFV87_20445"
     CDS             3049..4455
                     /locus_tag="VFV87_20445"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106206.1"
                     /translation="MNAALHLLTRRKLIQFAGGSLGLSLGGLLRAGAATAPVNSSMPP
                     LRSCIFIFYYGGPSHLETFDLKPDAPTDTRSQFKPISTSVPGLQIGEHLPKLSKWMHK
                     AALIRSMSHQARLHDSASIHMLTGRPLEGPDRELFAPLPQFYPSYGGAVSYMRRDEEH
                     VLPFASLPFVYHNVVPTPSQGGGFLGNGYDPLRIEVDPANRAYRADSFQPLEGLSPAR
                     MSDRRQLLESIDPTSAIGKQMRDDLDRAYQLLSSEAIRSAVDITREPAAVREKYGFGS
                     EPVAVGEGGGGGNGAEMGYARFMRGQNLLLARRLVEAGVPFVNVYDFKQQGQNWDAHF
                     NNAGQHKNQLLPQADQARATLIEELDERGLLESTLIVGCGEFGRTPKINAQGGRDHWP
                     DCYSVFLAGGGVSGGAVYGASDRLAAYPARDPATPGDLAATIYWRFSLNHAAEIHDQT
                     GRPHRLAVGEPIKTLFGA"
     gene            complement(4452..4829)
                     /locus_tag="VFV87_20450"
     CDS             complement(4452..4829)
                     /locus_tag="VFV87_20450"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HEX5106207.1"
                     /translation="MGATQVTVSVVNPADPARRWDGLFLVDTGSIDSYVPGNRLRDIG
                     IQPAGQRTYEMADGTEIALDIAVAQIEFMGEVVGATVIFGNDDTEPLLGVTALESVGI
                     EVDPQNQRLKRLPAVRLKRLNRP"
     gene            4903..6303
                     /locus_tag="VFV87_20455"
     CDS             4903..6303
                     /locus_tag="VFV87_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106208.1"
                     /translation="MITNHLSRRSFLATSTAAAAGLWLAGRDVPLLGQEKGERPKQDP
                     SVKVLNPQARVPLSFIIDDSTCLVNLAHFCIPQFAEVFPDRYKQDWRKLPREIPDSFV
                     REFGEWCKGHGVKGKYSIVPYPAMVGWVDREMPGWTRRELEESVKLVRDFMCPDWDIH
                     PEMVTHTWAINTKTGRPYDERTERFMENWGFSVGKSVDELTDYLAFALKILKNAGLPC
                     EGITTPGGFGSRVMPELAQATLQSCRDVYGVSLAHYFRNLYTDDRSVAPQVQYASGLE
                     TGDPKCVVSVIGCTGDWFGGWDGLEAGSVDQFITADLKGGRLPQVIDRGEPAILVCHW
                     PGIYFNGERIGFNIFKEVVKRLNDRYDNLLWMKNSEIARYWAAKELTAISREGNRVTL
                     TAPFATTRYTLSIAVPDSAAIQRVTIGPTGSDVGSNLDEVDAALKLKPGTWHRGRGGI
                     MACFDLSKGKMRVELA"
     gene            complement(6434..6766)
                     /gene="trxA"
                     /locus_tag="VFV87_20460"
     CDS             complement(6434..6766)
                     /gene="trxA"
                     /locus_tag="VFV87_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5106209.1"
                     /translation="MAGAGVLEFTDGNFQAEVLDSNQPVLVDFWAPWCGPCRRIAPMI
                     DELAAENQGAAKIGKVNTDDNNAVSMQYGIDAIPTLILFKNGQPVQRFQGIPAKSRVQ
                     EALDAAKA"
     gene            6992..>7906
                     /locus_tag="VFV87_20465"
     CDS             6992..>7906
                     /locus_tag="VFV87_20465"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HEX5106210.1"
                     /translation="MTASSLRCFLVEKDAAGNVQRGIAQRSLADLPPGEVFVRVQFSS
                     LNYKDALAAQAHPGVVRNLPHVPGIDAAGTIAESSDARFKPGQAVVITGYGLGEGQWG
                     GWAEYVRVPAGWIVSLPAGLSLNEAMILGTAGFTAAQCVQAIQLNGVQPAAGEIVVTG
                     STGGVGCIAVQLLAKLGYSVVAVTGKPQLESRLKQWGASRIIGRGDVVNSSDKPLLAA
                     KWAGAVDCVGGDTLATILRETKPYGVVSACGLVGGANLPLTVHPFILRGVMLAGIGSA
                     LLPYDRRLEIWNKLSGPWRLDGLESFAQT"
BASE COUNT         1420 a         2509 c         2521 g         1456 t
ORIGIN      
        1 cccgatcgct ggtggagcta cgaaggggtc gatgccatcg tgctcgctac gagcgatgag
       61 gccttcctgg acaagctgac gtcgccacag atcgacgcaa tcgcacagtg ggtgcggctc
      121 ggcggtcggc tgattctgtg cgttggcgcg cgcggagaga agctgttggc ggagggcggc
      181 ccctgggctg cgctcgctcc gggcaagctc gccgatgtct caccccttcg cgacggcacg
      241 gggctggaaa cgttcaccgc cagcaagctg ccgcatggcg aagagcgata cgagcgcgat
      301 cgtccgctcg tcagccgctt gacggacgcc agcggcaccg tgctggtcga agaaggcggc
      361 gccatttcgg caaatcgtcc gctgatcgtg catgcgccgc gcggcctggg gcaagtgacc
      421 tacgtcggga tcgatctgga tcatcccgcc ctggcttcgt ggaagggccg gccgagcttg
      481 atcgcccggc tgctgcaagg caaggagccg cgggagcaga gcgaccgcga agccaaccgc
      541 agcgtcacac atttgggcta cgaagatctc gtcggccagt tgcgggctgc gctcgatcaa
      601 gtcccgaccg tcaagatcgt gaatttcacg accgtgtcga tcctgacgat cgtctatctc
      661 ctgctgattg gtcccggtga ttttctgctc ctcagccgac tgggctggcc gcgacaggtg
      721 acgtggttca catttccgct cgtcgccatt gccctgggcg cggtggcgtg gctcttcggc
      781 agccagtcgc acggtgaaaa agtgcgactg aatcaggcgg agctggtcga tatcgatgcg
      841 tcttcgggcg tcgcacgcgg cacggtgtgg gcccatctat acagtcccgc gaccgaccac
      901 ttcgatctgc agatgcaaat cgagccgccg ccgacttcgt cgccgggcaa tgccgcccag
      961 ccgcaaggtt ggctggcgtg gcagggcctg ccgggcgaat cgatgggtgg actggcgtca
     1021 cggcaggtga gcctggcggc ggcagacccg tattcaatca gcttgccccg gccgcagcca
     1081 acgatggaga acctgccggt tcaggttgcc tcgtcgaaga gcctgtccgg ccggtggtgg
     1141 gcgcaggtgg acgtgcaggc caagaatcag ctcgccgaga atcaatacgg tgtggtcacg
     1201 ggcaccttcg agcagccgct gccctttgag ctgaccgatt gcctgctgat ccacggtgaa
     1261 aagctctacc ggctggggaa actgccagcc gggcacacgg ttcatatgga cgattttgct
     1321 ccgctcaatc tggaagcccg cctcacggaa cgcaccgtgg tgctgtcgaa agacgtctcc
     1381 acgccgtggc ggaaagacct cgttgacgtg ccgcggatcc tcctgatgat gatgtttcac
     1441 gaggccgcac gcggcagcag ctttaccggc ctgaccaacc gctatcagcc ctacgtcgat
     1501 ttgaccgagc aagtgcggct ggggcgagcg gtcctcgtcg gccggactga gaagggcgcc
     1561 tgccagattc accggggcga tcggcccctc gccgatccaa gcgccgtgca gacctggacc
     1621 tggtatcggg ttattctgcc cgttggaaaa gagcaacctg agcagccaac gaatccgtag
     1681 gccgggtttc caacccgtcc gatctggccg ggttgaaaac ccggcctgcg agctcgccgt
     1741 gatcaagacg cacaacctga cgaagaaata cggcgaatcg tacgccatcc aggcgatcga
     1801 gctggatctc gcggcgggcg atttgttcgg cttcatcggc cccaacggtg cgggcaaaac
     1861 gacgacgatg aagatcctcg cgacgctgct caatccgacc tatggcgagg cctacgtctg
     1921 cggccactcg atctatcgcg atccgaagga aattcgtcgg ctggtcggct acatgcccga
     1981 ttttttcggc gtgtacgacg atatgaaggt cattgagtac ctcgaattct tcgccgccgc
     2041 ctaccgcatc aacggcagca agcggcggca ggtctgcaac gagaagctcg acgtcgtcga
     2101 tctcgatttc aagcgcgacg cttacgccaa tacgctcagc cgcgggcaga cgcagcgcct
     2161 gggcctggcc cgcgtccttt tgcacgagcc gcaggtgctg ctgctcgatg agccgctctc
     2221 gggcctcgat ccgcgggcgc gaattgagat gcgggcggtg ctgcggcgtc tgggccagca
     2281 gggcaaaacg atcatggttt cgagccacat cctgcccgag ctggccgaca tgtgcaacaa
     2341 ggtcggcatc atcgaccgcg gcgtgatgac ggtgaacgcc acggtggccg acgtgatgaa
     2401 gcaagtccgc gagcggattg tgttgcacgt tcaagtcact ggcgatctgg atgcagcggc
     2461 ccggctgctg gagcagaacg acctggtcga aagtgtcgca attgtcaaag agcagatcgt
     2521 cgtcacgctc aagaaaggcg tcgaggacta cagcgatctt tcaccgctgc tcatctccgc
     2581 cggccacaag attaagctct ttcgcgagga agaggtcaac ctggaatcgg catttatggc
     2641 gctcaccaag ggtctcggtc agcgcgtctg acaatgacgg actggctcct catgtggctg
     2701 cgggtgcaag ctggagcttt tgcagcattc cgcggacgag ttgttcctga ttttgcaggt
     2761 gtcggcgaaa ttccgtagag aattctgtgc gtcgaacttc cacgcggcga ttctcgagcg
     2821 actcctgcag gatttccacc agcaggtcgc gctcgccctg cgtcaacgaa aactgaatca
     2881 tggccttggt cctccagtgc gtgttgtggc cattaagcat gcccctcaac aacttgcaaa
     2941 gtctgtgcca gaaaactggt ttttccggcc gtttcttgac gccgttcaat aatccgggca
     3001 aacttagcaa acccgccacg ccgctccaga tcccgccggg agccacggat gaacgctgcc
     3061 ctccaccttt tgacgcgccg caaactgatc caatttgccg gcgggtcact gggcctttca
     3121 ctcggcggct tgctccgtgc tggggcggcg acggctccgg tcaattcttc gatgccgccg
     3181 ctgcggtcgt gcatcttcat tttctattac ggcgggccga gtcatctgga aaccttcgac
     3241 ctcaagcccg atgcgccgac cgacacccgc agccagttca aacccatttc aacgagcgtt
     3301 ccgggcctgc agatcggcga gcacctgccg aagctctcga aatggatgca caaggcggcc
     3361 ctgatccgca gcatgtcgca ccaggcgagg ctgcacgatt ccgcgtcgat ccacatgctt
     3421 acgggaaggc cgctggaagg gcccgaccgc gagctcttcg ccccgctgcc gcagttctat
     3481 ccgagctatg gcggggcggt gtcgtatatg cggcgggatg aggagcatgt cctgccgttc
     3541 gcctcgctgc cgttcgtcta tcacaacgtc gtgccgacgc cttcgcaagg cggcggattt
     3601 ctcggcaacg gttacgatcc gctgcggatc gaagtcgatc cggcgaatcg cgcctaccgg
     3661 gccgattcgt tccagccgct ggaggggctg agtccggcgc ggatgtcgga ccggcggcag
     3721 ttgctggagt cgatcgatcc gacgagtgcg atcggcaagc agatgcgcga cgatctggac
     3781 cgggcctatc agcttctcag ctccgaggcg atccgcagcg ccgtcgatat cacccgcgag
     3841 ccggccgcgg tccgcgagaa atacggcttt ggcagcgaac ccgtcgcggt gggcgaaggg
     3901 ggcggcggcg ggaacggggc ggagatggga tacgcccggt tcatgcgcgg gcagaacctg
     3961 ctgctcgctc gccggctggt cgaggcgggc gtgccgtttg tcaatgtcta tgacttcaag
     4021 cagcaggggc agaactggga cgcccacttc aacaacgccg gccagcacaa gaaccaattg
     4081 ctgccgcagg cggatcaggc gcgggcaacg ctgatcgaag aactcgacga gcgggggctc
     4141 ttggaaagca ctttgatcgt cggctgcggc gagttcggcc gcacgccaaa aatcaacgcg
     4201 cagggcggcc gcgaccactg gcccgattgt tacagcgtgt tcctggccgg cggcggcgtc
     4261 agcggcggcg cggtctatgg cgccagcgac cgcctcgccg cgtacccagc ccgcgatccc
     4321 gccacgcccg gcgacctcgc cgccacaatc tactggcgct tcagcctcaa ccacgccgcc
     4381 gaaatccacg accaaaccgg ccgcccgcat cgcctggccg tgggcgagcc gatcaagacg
     4441 ctattcgggg cttagggacg gtttagccgt ttcagcctca ccgccggcag ccgcttcagg
     4501 cgctggttct gcggatcgac ttcgatgccg accgattcga gggccgtaac gccgagcagc
     4561 ggctcggtgt cgtcgttgcc gaagatcacc gtggcgccga cgacttcgcc catgaattcg
     4621 atttgcgcca cggcgatgtc gagggcgatc tccgtgccgt cggccatttc gtaagttcgc
     4681 tgtcccgccg gctgaatccc aatatcccgc agccggttac ccggcacata gctgtcgatt
     4741 gagcccgtat cgacgagaaa cagcccatcc cagcggcgcg cgggatcggc tggattgaca
     4801 acggaaaccg ttacttgcgt ggcacccatg tcgggattct atacgcccca ccgactgccc
     4861 gcaaattgcg atcactgtgc cggtcacgtt agattagcgg acatgatcac taaccacctc
     4921 tcccgccgct cctttctcgc cacttccacc gccgctgccg ctggcctctg gctcgcgggc
     4981 cgagacgtgc cgctgctcgg ccaggaaaaa ggcgagcggc cgaagcagga tccgagcgtc
     5041 aaggtgctca atccgcaggc gcgggtgccg ctgtcgttca tcatcgacga ctcgacctgc
     5101 ctggtgaacc tggcccactt ctgcatcccg cagttcgccg aggtctttcc tgatcgctac
     5161 aagcaggact ggcggaagct gccgcgggag attcccgaca gcttcgtccg cgagttcggc
     5221 gagtggtgca aggggcacgg cgtcaaaggc aagtacagca tcgtccccta tccggcaatg
     5281 gtcggctggg tcgatcgcga gatgcccggc tggacgcggc gcgagctgga agagagcgtc
     5341 aagctggtgc gggacttcat gtgcccggac tgggacatcc atcccgagat ggtcacgcac
     5401 acctgggcca tcaacaccaa gaccggccgc ccgtacgacg agcggaccga gcggttcatg
     5461 gaaaactggg gcttctcggt cggcaaatcg gtcgatgagc tgaccgacta cctcgccttc
     5521 gccctcaaga ttctgaagaa cgccggcctc ccctgcgaag gaatcaccac gcccggcggc
     5581 tttggcagcc gggtgatgcc ggaactggcc caggcaacgc tgcaaagctg ccgcgacgtg
     5641 tacggcgtca gcctggctca ctatttccgc aacctgtaca ccgacgaccg cagcgtcgcc
     5701 ccgcaggtgc aatacgccag cggcctggaa acgggcgatc ccaagtgcgt cgtctcggtc
     5761 atcggctgca cgggggactg gttcggcggc tgggacggcc tggaagccgg ctcggtcgat
     5821 caattcatca cggccgatct caaaggcggc cgactgccgc aggtcatcga ccgcggcgag
     5881 ccggcgatcc tcgtctgcca ctggcccggc atctatttca acggcgagcg gatcggcttc
     5941 aacatcttca aggaggtcgt caagcgcctg aatgatcgct acgacaacct cctctggatg
     6001 aaaaacagcg aaatcgcccg ctactgggcc gccaaggagt tgacggcgat cagccgcgaa
     6061 gggaaccgcg tcacgctcac cgcccccttc gccaccacgc gttatacgct gagcatcgcc
     6121 gtgcccgact ccgccgcgat ccagcgggtc accatcggcc ccacgggcag cgacgtgggc
     6181 agcaacctgg acgaagtcga tgcggccttg aaactcaagc cgggcacctg gcaccgcggc
     6241 cggggcggga ttatggcctg cttcgactta tcgaagggca agatgcgcgt ggaactcgcc
     6301 tgatcattcc attagccggg agcaaactaa acttgctctc ggagggcgga cggtggatgc
     6361 tccattcgca gttctccggg accaggtttg gtcccggcta agaacattgg tttggtcccg
     6421 gctaagaaat cgtttacgcc ttcgcggcgt cgagcgcttc ctgcacgcgc gatttggcgg
     6481 gaatgccctg gaagcgctgc acgggctggc cgttcttgaa gaggatcagc gtggggatcg
     6541 cgtcgatgcc gtattgcatc gaaaccgcgt tgttgtcgtc ggtgttcacc ttgccgatct
     6601 tggcggcccc ctggttctcg gcggccagct cgtcgatcat cggggcgatg cggcggcacg
     6661 ggccgcacca gggggcccaa aaatcgacca gcaccggctg attgctatcg aggacttcgg
     6721 cctgaaaatt gccatccgtg aattcgagta cgcctgctcc ggccatgatt ttttctccgt
     6781 gaatcgtcga gttattgcta ccggcaaaat tgttgcttgg cggacaagtg gcgtgatttt
     6841 agaagcgcgc cagaggaagc gtcaaccggc ccgcgttacc gtcgcgatgt cgtaagtcgt
     6901 tgggctgctt gcagctacgg cgcgcggcct ctaggttggc cggtggcgat ctttgaatga
     6961 gcccgtacgc ggacacttta ttgagagacc gatgactgcc agctcgctcc gttgcttcct
     7021 cgtcgaaaaa gatgctgccg gaaacgtgca gcgcggcatc gcgcagcgct cacttgcgga
     7081 cttgccgccg ggcgaggtgt tcgtccgcgt gcaattctcg tcgctcaatt acaaggacgc
     7141 acttgccgcc caggcccatc cgggcgtcgt ccgcaacctg ccgcatgtgc cgggaattga
     7201 cgccgcggga accattgccg aaagcagcga cgcgcgcttc aagcccggcc aggccgtcgt
     7261 catcaccggc tatgggctgg gagagggcca gtggggcggc tgggcggagt atgtccgtgt
     7321 gccggccggc tggattgtct cgctgccggc gggattgtcg ctaaacgagg cgatgattct
     7381 cggcaccgcg ggctttaccg cggcgcagtg cgtgcaggcg attcaactta atggcgtcca
     7441 gcctgctgcg ggcgaaatcg tcgtcactgg ctcgacgggc ggcgtcggct gcatcgccgt
     7501 ccaattgttg gccaagctcg gctattcggt cgtcgccgtc accggcaagc cacagctcga
     7561 atcgcgcctg aagcagtggg gcgcgtcgcg aatcatcggc cgcggcgacg tcgtcaattc
     7621 cagcgacaag ccactgctgg ctgcgaagtg ggctggcgcg gtggattgcg tcggcggcga
     7681 cacgctggcg acgatccttc gcgaaacgaa gccttacggc gtcgtctccg cctgcggact
     7741 ggtgggtggc gcgaacctgc cgctcaccgt ccaccccttt atcctccgcg gcgtcatgct
     7801 ggccggcatc ggctcggcgc tattgcccta cgatcgccgg ctggagatct ggaacaagct
     7861 cagcggcccg tggcgcttgg atggcctgga gtcatttgcg cagacg
//
LOCUS       DASUGB010001080         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1649950, whole genome shotgun sequence.
ACCESSION   DASUGB010001080 DASUGB010000000
VERSION     DASUGB010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649950"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFV87_20470"
     CDS             complement(<1..373)
                     /locus_tag="VFV87_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106211.1"
                     /translation="MLRAFLCSLLAVMAIAPLAQARGRRSGWSEESHQPKLELTEVQY
                     TDHRDRKVDGSAGYNADLVTDLPQASEQKLYLTLTTAAQPTQRDLQVAEWFATDPRLV
                     HFRTQTHWNWYDTANPHYRERL"
     gene            complement(382..576)
                     /locus_tag="VFV87_20475"
     CDS             complement(382..576)
                     /locus_tag="VFV87_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106212.1"
                     /translation="MAVWIHSLVLLLLLPCLCGGCGLHAWHAWRLFVIEDWCEHAIGW
                     WPWDPFEHEEVNPKFPDLPG"
     gene            complement(582..1226)
                     /locus_tag="VFV87_20480"
     CDS             complement(582..1226)
                     /locus_tag="VFV87_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106213.1"
                     /translation="MRRALLLLTTLAATCAAGCEVEFTLSEGDVPRPQPAPTPRVVEV
                     PVANLPVSLRVRNYKGGSCVHASTGSGFNWCNLLKLALHWNSAYSGGESYNGLTSKLK
                     RNGIPFYDTASGDVSVLERATRERRMPTIFYYSNHSVSFCGFGDGAVRAGSRWGFVRN
                     PQGKFAYLLDNNRTGAFIEIPRDEFIRRWRGYGGVAVVPTLGAPAPPLPFIARN"
     gene            complement(1202..1468)
                     /locus_tag="VFV87_20485"
     CDS             complement(1202..1468)
                     /locus_tag="VFV87_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106214.1"
                     /translation="MRRLAQLLAVLRWLNQSVWLRWLLGLVVAIATLWATCRPKCCDE
                     PQPAPPPRPRPILPWRRDESTDERDESPSPRNAEIQRCDVPYCY"
     gene            complement(1468..1674)
                     /locus_tag="VFV87_20490"
     CDS             complement(1468..1674)
                     /locus_tag="VFV87_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106215.1"
                     /translation="MGKTFGEQVFSIVKGAGLAAGGAALAYFGSYAAGHPTDLLGLLA
                     AGVASVGLNILRKFGGGVVAELLK"
     gene            complement(1772..>2147)
                     /locus_tag="VFV87_20495"
     CDS             complement(1772..>2147)
                     /locus_tag="VFV87_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106216.1"
                     /translation="ASLRTAALTLRAEGYRASKSVLGRLAREHRLGSRAERLSRETWR
                     AIDGRLARGVPLRQIAREVPAASLTSIRRRAAAKLWAEALREKLDVVHRRTTQWRCPR
                     GALVTTDPCVCCGARRPRAAGQ"
BASE COUNT          346 a          802 c          683 g          316 t
ORIGIN      
        1 aaagccgctc gcgatagtgc gggttggccg tgtcgtacca gttccaatgc gtctgcgtcc
       61 ggaaatgcac cagccgcgga tcggtcgcaa accactcggc gacttgcaga tcgcgctgcg
      121 tcggttgggc ggccgtcgtc agcgtcagat acagtttctg ctcgctcgcc tgcggcaggt
      181 cggtcaccag gtccgcgttg tagccggccg acccatccac cttccgatcg cgatgatcgg
      241 tgtactggac ttcggtcagc tccaacttcg gctgatgact ttcctccgac cagcccgatc
      301 gccggccgcg ggcctgggct aatggcgcga tggccatcac ggccaagaga ctgcacaaga
      361 acgctcgcaa catcgcagct cctaccccgg caggtccggg aacttggggt tcacttcctc
      421 gtgctcgaac ggatcccacg gccaccagcc gatcgcgtgc tcgcaccagt cctcgatcac
      481 aaacagccgc caggcgtgcc aggcgtgcag cccgcacccg ccgcacaggc aaggcagcag
      541 gagcaacagc accaggctat ggatccacac cgccacgcgc atcagttcct cgcaatgaac
      601 ggcagcggcg gcgccggcgc gccgagcgtg ggcaccacgg cgacgccgcc gtagccgcgc
      661 cagcggcgaa tgaactcatc gcgcgggatc tcgatgaaag cccccgtgcg gttgttgtcg
      721 agcaggtagg cgaacttccc ttgcgggttg cggacgaagc cccaccgcga accggcgcgc
      781 accgccccgt ccccgaagcc gcaaaaggag acgctgtgat tcgagtaata gaagatcgtc
      841 ggcatgcggc gctcgcgcgt cgcccgctcc agcacgctca cgtcgccgct ggccgtgtcg
      901 tagaagggga tgccgttgcg cttcagcttc gacgtcaggc cgttgtagga ttcgccgccg
      961 ctgtaggcgc tgttccagtg cagggcgagc ttcagcagat tgcaccagtt gaagccgctc
     1021 ccggtgctcg cgtgcacgca gctcccgccc ttgtaattgc gcacccgcaa agaaaccggc
     1081 aggttggcga ccggcacctc caccacccgc ggcgtcggcg ccggctgcgg acgcggaacg
     1141 tccccttcgc tcagcgtgaa ctcgacctcg cagccggccg cacaggtcgc ggcgagggtc
     1201 gtcaatagca gtagggcacg tcgcatcgct ggatctcggc gtttcgtggg gagggcgatt
     1261 cgtctcgctc gtcggtgctc tcgtctcgtc gccagggcag aatcggccgc ggcctgggcg
     1321 gaggcgctgg ctgcggctcg tcgcagcact tcggccggca cgtcgcccac agcgtggcga
     1381 tcgcgacgac caggcccagc agccagcgga gccaaaccga ctggttcagc caccgcagca
     1441 ccgccaggag ctgcgcgagc cgccgcatta cttcagcagc tcggcgacga cgcccccgcc
     1501 gaacttgcgg aggatgttca agccgaccga cgccacgccg gcggccagga ggccgagcag
     1561 atcggtcgga tgcccggccg catagctccc gaaataggcc aatgcggccc cgccggccgc
     1621 caggccggcc cccttcacga tcgaaaacac ctgctcgccg aacgtcttgc ccacgggcat
     1681 gctccagaaa gaaatggacc ggtcgaaaga agcctgctgc gtcaggcccc attctcggca
     1741 gcccccgccg ggcgcgccgc tgtcccgcgt ttcactgtcc cgccgccctc ggccgccgtg
     1801 ctccgcagca gacgcacgga tcggtggtca ccagcgcccc gcgcggacaa cgccactgcg
     1861 ttgtgcggcg gtggacgacg tccagcttct cgcgcagcgc ctcggcccag agcttcgccg
     1921 ccgcccgccg gcggatgctg gtcaggctcg ccgccggcac ctcccgggcg atctgccgca
     1981 gcggcactcc gcgcgccagc cgcccatcga tggcccgcca cgtttcccgg gacaaccgct
     2041 cggcacgcga ccccagccgg tgctcccgcg ccagccgccc caggacgctc ttgctggccc
     2101 ggtaaccctc cgctcgcagc gtcagggccg ccgtccgcag gctcgct
//
LOCUS       DASUGB010001081         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1231187, whole genome shotgun sequence.
ACCESSION   DASUGB010001081 DASUGB010000000
VERSION     DASUGB010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231187"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1202
                     /locus_tag="VFV87_20500"
     CDS             357..1202
                     /locus_tag="VFV87_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106217.1"
                     /translation="MATSGRWFADWTIIGLSTAVGAMAQVPEPDLSSDAAKEFQAYAK
                     GCAAAYDIQVQAQEGEPRQLALTAEPILRWTNPLAGRQAHGEVFLWTDDGRPTAVLSL
                     YQWTAPDKAVHEHHEFCSLATASLATAGPEKRDWSPQAAGVQFLTLPESPVPADSPRL
                     RLIQLKDLAARFTAEKTTREDQEVRDLRLLARPIQRYESKQNDVIDGALFAFVEHTDP
                     EIFLILEARLAGGKSEWQYACVRMNSLRLAVALDGKSVWEAEQLPWREALNRPDLPYT
                     AFTIR"
     gene            complement(1205..1942)
                     /locus_tag="VFV87_20505"
     CDS             complement(1205..1942)
                     /locus_tag="VFV87_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106218.1"
                     /translation="MAKKKRPARPTAKKAPKKGKKPARGKTKPKAKAQAARKPAKKKA
                     AAAKSVAKKRPVKKKAAKPAKKSGASNPSKRTELGAKNVGEPIWELKAATAAVLREPE
                     FQSVRGDAIDYYFEKFLMSFLPQAIDEALNDAQLANLSPAAVRAACFLLVGDMIRKLN
                     RRLNPSAAADQTGWWQEKLLVRLAAAAGVPAESYVQSVLEIKDPEDDAPALVERVAQS
                     VGLSPNSLFMFHTMMIEVVEEDVLDEH"
     gene            2053..2847
                     /locus_tag="VFV87_20510"
     CDS             2053..2847
                     /locus_tag="VFV87_20510"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5106219.1"
                     /translation="MSWIDRLWISLVAIPGAMLERGFAYVAIAGFAWVVLHVILARWL
                     ARRRIGDKAIGLGQVSREVLYSLRSLAIYGLVGGCMVFAYLAGWTQIYLRFERYGWPW
                     FFISIGLVILIHDAYFYWTHRLMHHPRLFRLFHRTHHLSLSPSPWAAYAFSPWEAFVQ
                     AGIAPLVLFTLPVHPLAFSIFMLWQISFNVLGHCGYELFPRWFVRSPLGLVLNTATHH
                     AQHHETNRANFGLYFNIWDRLMGTNHARYAERYDEAVGKAELSSAP"
     gene            complement(2922..3749)
                     /locus_tag="VFV87_20515"
     CDS             complement(2922..3749)
                     /locus_tag="VFV87_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5106220.1"
                     /translation="MRFAICNETFQDWPFEKAFAFAAELGYTGLEIAPFTINTNAYQI
                     SAAKRAEVRKQAEAAGLEIIGLHWLLAKTTGYYLTTPDDPVRGKTADYLAELARLCRD
                     LGGSVMVLGSPMQRNLLPGVSHEQAMELAADCLRRAMPTLEDCGVTLAVEPLGPKDGT
                     FLLTADLGARLCEMVASPHCRLHLDCKAMASESKPIPQILREQAKLLAHFHANDPNMR
                     GPGMGDLDFVPIFRALAEIDYRGWVSVEVFDYEPGVEALARESIEYMEECVAKLATQ"
     gene            3934..>4337
                     /locus_tag="VFV87_20520"
     CDS             3934..>4337
                     /locus_tag="VFV87_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX5106221.1"
                     /translation="MSDSPAKPTNLRQLREIGWQSKPVKREIHDNFLAMLARGDELFP
                     GIVGYDSTVIPEINLALLSGHDQLFLGEKGQGKSRLMRSLVRFLDAEIPYLDIPGVAV
                     HEDPYKPITTVGRRFVADHNEDHIPIGWWRRE"
BASE COUNT          741 a         1417 c         1326 g          853 t
ORIGIN      
        1 aggagcgtgc cgaccagcgc gagcaaaatg ccgccggcaa agaccgccag cgacagccgc
       61 caacgccggc ggagggctct ctccagttgc tggtaaagat cgcgaatctg ctggacgttg
      121 atcatggaaa atcggcttgg cgaaggtgcg gtcggcgcgc gaagggacac aggtacttac
      181 ggcgcaggtc ctaaggacga aggaccgagg acgaaggacg aaggctgcgc gaggtgtgca
      241 gacgccgatt ctgccggact tgacggctgc gccgttgaca gcgccgatcg atcgggcgta
      301 gcctcggatt aagacattac gtcttatccg ctcctttttg aacgtcgagg ctcgtcatgg
      361 caacttctgg acgctggttt gccgattgga cgatcatcgg actatccacc gcggtcggcg
      421 ccatggcgca agtgcctgag ccggatctct cttccgatgc cgccaaggag tttcaggcct
      481 atgccaaagg atgcgccgcg gcctacgaca tccaggtcca agcgcaagaa ggcgagccgc
      541 ggcagctcgc gctgactgct gagccgatcc tccgctggac aaatcctttg gccggaaggc
      601 aagcgcacgg cgaggtgttc ctgtggaccg acgacggccg gccgaccgcc gtcctctcgc
      661 tctatcagtg gactgcgccg gacaaggccg ttcacgagca tcacgagttc tgctcgctgg
      721 caacagcctc actggcgacc gcgggccccg agaagcgcga ctggtcgccg caggccgccg
      781 gtgtccagtt cctgactttg ccagagtccc ctgtgccggc ggattcgccg cgcctgcggc
      841 tgatccagtt gaaggacctg gccgcccgtt ttacggccga gaagacgacc cgcgaggatc
      901 aggaagtccg cgacttgcga ctcctcgcgc ggcccataca gcggtacgaa agcaagcaga
      961 acgacgtcat cgacggggcg ctctttgcct tcgtcgagca caccgacccg gagatttttc
     1021 tgatcctcga agcccgactc gccgggggca agagcgaatg gcaatatgcg tgcgtgcgga
     1081 tgaacagcct ccgcctggct gtcgcactcg acggcaaatc ggtctgggaa gcggagcaac
     1141 tgccttggcg cgaggccctc aaccgccccg acttgccgta caccgccttc acgatccgtt
     1201 gaagtcaatg ttcatccagc acatcctcct cgaccacctc gatcatcatc gtgtggaaca
     1261 tgaacaagct gttgggtgac aggccaacgc tttgagcgac tcgctcgaca agcgcggggg
     1321 cgtcatcctc agggtctttg atttccaaga ccgattggac gtacgactcc gctggcacgc
     1381 cggctgccgc ggcgagcctt acgagcagct tctcctgcca ccagccggtc tgatcggcgg
     1441 cagcgctggg attcagccgc cggttcagct tacggatcat gtcgcccact agcaggaagc
     1501 aagcggccct gaccgcggcg ggcgacagat tggcaagctg cgcgtcgttg agtgcctcgt
     1561 cgatcgcctg cggcaggaag ctcatcagga acttttcgaa gtagtagtcg atcgcgtccc
     1621 cgcgcacgct ctgaaattcc ggctcgcgca gcacggcggc ggtagcggcc ttcagctccc
     1681 aaatcggctc gccaacattc ttcgctccca gctccgtgcg cttggacgga tttgaggcgc
     1741 cagacttctt cgcgggcttc gcagctttct tcttcacggg acgtttcttc gcaaccgact
     1801 tcgctgcagc ggcctttttc ttggcgggct ttcttgcggc ctgcgccttg gccttgggct
     1861 tggtcttgcc gcgagctggt ttcttgccct tcttgggtgc ctttttagcc gtgggacggg
     1921 cggggcgttt tttcttggcc atgatgtggt actcgcgaga aggaaagagg agcaagccgc
     1981 cagctattct ggtcgatagc tgcgcgacaa gccagcgtgg tatcctgagc ggatctctcc
     2041 ccgggtgctg ctatgtcctg gatcgaccga ctctggattt cccttgttgc cattcccggc
     2101 gcgatgctcg aacgcggctt cgcctatgtc gcgatcgccg gctttgcgtg ggttgtgctg
     2161 cacgtgattc tcgcccgctg gttggcgcgg cggaggatcg gcgacaaggc aatcggtctt
     2221 ggccaggtca gccgcgaggt gctgtactcg ctgcggagcc tggctatcta cgggctcgtt
     2281 ggcggctgca tggtcttcgc ctacctggcg ggttggacgc agatttatct gcgattcgag
     2341 cgttacggct ggccgtggtt tttcatcagc atcggcctgg tcatcctgat ccacgacgcg
     2401 tacttctatt ggacgcaccg cctgatgcac cacccgcggc tgtttcgcct cttccaccgc
     2461 acgcaccatc tctcgctcag tccctcgccc tgggccgcgt acgccttcag cccgtgggag
     2521 gccttcgtcc aggccggcat cgccccgctg gtcctcttta cgctgcccgt gcatccactg
     2581 gcgttctcga tcttcatgct ctggcagatt tcgttcaacg tgctggggca ctgcggctac
     2641 gagctgttcc cccgctggtt cgtccgctcg ccgctggggc tggtgctcaa cactgccaca
     2701 caccacgccc agcaccacga aaccaaccgg gccaacttcg gcctctattt caacatctgg
     2761 gaccgcctga tgggcaccaa ccacgcgcgg tatgcggagc gatacgacga ggccgttgga
     2821 aaggcagaac tgtccagcgc gccgtagccg cactcgccag agtgcgggca accggcaacg
     2881 cccccccgtt ctggcgaacg cggctacggc tggtgccgga gttattgcgt cgccagcttc
     2941 gctacgcact cttccatgta ctcaatgctc tcccgcgcga gggcttcgac gccgggctcg
     3001 tagtcgaaga cttcgaccga gacccagccg cgatagtcga tctcggccag cgccctaaag
     3061 atcggcacga aatcgaggtc gcccatgccg gggccgcgca tgttcggatc gttggcgtgg
     3121 aagtgcgcga ggagcttcgc ctgctcgcgg agaatctgcg ggatcggctt ggattcgctg
     3181 gccatcgcct tgcaatccag gtgcagccgg cagtggggcg aagcgaccat ctcgcagagc
     3241 cgggcgccga ggtcggcggt cagcaggaac gtgccgtcct tgggaccgag cggctcgacg
     3301 gccagcgtca cgccgcagtc ttccagcgtc ggcatcgccc ggcggaggca atcggcggcc
     3361 agctccatcg cctgttcgtg cgaaacgcct ggcaagaggt tccgctgcat cggcgagccc
     3421 agcaccatca cgctgccccc cagatcgcgg cacaggcggg ccagctcggc aagataatcg
     3481 gctgtcttgc cccgcacggg atcgtccggt gtcgtcaggt agtagccggt cgtcttcgcc
     3541 aagagccagt gcaggccgat gatttccagc cccgccgctt ctgcctgctt gcggacctcc
     3601 gcccgcttag ccgccgaaat ctggtaggcg ttcgtgttga tcgtaaacgg cgcgatctcc
     3661 agtcccgtat agcccagctc ggccgcgaag gcgaacgcct tctcgaaggg ccagtcctga
     3721 aaggtctcgt tgcagatggc gaagcgcatg gattctcgtt cagccagtgg gttgtgagtc
     3781 agcgtacaaa catttcgctc cggccattct atccgacgcc tcgcgaacgc tcatccgtgc
     3841 gggtctgtgt ttcatccgtg gctaccgccc gcccgacgtt tcgcaaggct ctataataag
     3901 ggggcatcct cctcgtaagc actgagcctt cgcatgtcgg attctcctgc caaacccacg
     3961 aatctccgcc agcttcgcga aatcggctgg caatcgaaac cggtcaaacg cgaaatccac
     4021 gacaatttcc tggcaatgct ggcccgcggc gacgagcttt tccccggcat cgtcggctac
     4081 gacagcacgg tcattcccga gatcaatctc gccctcctgt cggggcacga ccagcttttt
     4141 ctcggcgaaa aaggccaggg caaaagtcgc ctgatgcggt cgctggtgcg gttcctcgac
     4201 gccgagattc cgtacctcga tatcccgggc gtcgccgttc acgaagatcc ctacaagccg
     4261 attaccaccg tcggccgccg gttcgtggcc gatcacaacg aggaccacat cccgatcggc
     4321 tggtggcggc gcgagga
//
LOCUS       DASUGB010001082         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1092845, whole genome shotgun sequence.
ACCESSION   DASUGB010001082 DASUGB010000000
VERSION     DASUGB010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092845"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..276
                     /locus_tag="VFV87_20525"
     CDS             61..276
                     /locus_tag="VFV87_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106222.1"
                     /translation="MTIGDWLGLVCLAGLVGFFAGGFFVWNLFYKVPQPDDGSDDGPE
                     DDGPEDDEDESHKEDLAASESHQEPEI"
     gene            325..990
                     /locus_tag="VFV87_20530"
     CDS             325..990
                     /locus_tag="VFV87_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106223.1"
                     /translation="MSEPLSDFRLQSASAQPAAATTTPANEYPYQSPDSPEAPQIEMP
                     DTPAYSPLEAQIAAQFQQGKRGASWFYSIAGFSLINSIIVLFGGGTYFVIGLGVTVIT
                     DHFAVAAAQQSPDAAVLVKGGAFAFDLFVAAALCAFGWLSVKRWQPVFILGMTLYLLD
                     GLLFLMFGRMMAVAFHAYGLYCMWSGFQAYRRLADLERQLMDPLHPAGMLNPHQAAPL
                     EGT"
     gene            complement(1000..1353)
                     /locus_tag="VFV87_20535"
     CDS             complement(1000..1353)
                     /locus_tag="VFV87_20535"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5106224.1"
                     /translation="MTATATKYLRRDPAEVEPWRETCGQIRPLVEEKDQAAAEVHHVE
                     IDHAKLHYHARTDEFYYIIAGTGTMVLDDDEIDLHPGVVVYVPRGVKHKAVGKLTVLT
                     VCIPRGVLGDIVELE"
     gene            1462..>2202
                     /locus_tag="VFV87_20540"
     CDS             1462..>2202
                     /locus_tag="VFV87_20540"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106225.1"
                     /translation="MLNLGRQIAKVCGRTTRRELLRAGGLAAAGLSLADLLRLEARGA
                     SENEPKAKSVILLWLWGGPSHLDTFDMKPKAPVDYRGPYAPVPTKIQGIQICELFPLL
                     AARADRYSIVRSMHHTSNDHGIGGTIGLTGSDSGAISLSGQVLSGQVKATHGSVVSRV
                     LGFSPEMPRFVTMGGTLHQGKRSIAGESAGPLGALYDPFRLDYDVQQGVKIPSLDLID
                     GNSASGLESREGLRKRMDGLLRDLESSSA"
BASE COUNT          409 a          663 c          674 g          456 t
ORIGIN      
        1 cgctgctgaa aaaccagggg ggtttttcag caccccactt ttgggttagg gtccgaaacc
       61 atgaccattg gagactggct cggcctcgtt tgcctggcgg gactcgtcgg attttttgcc
      121 ggtggtttct tcgtctggaa cttgttctat aaggttccgc agcccgacga tggatcggac
      181 gacgggcctg aggatgacgg gcctgaggac gacgaggacg aaagccacaa ggaggacttg
      241 gccgccagcg aatcccacca agagccagag atatgataca agtcagtgcc tacagaccgg
      301 ctgagcggta aaccgaggat tgccatgagc gaaccgttat ctgatttccg gcttcagtcg
      361 gcttcggccc agcccgcagc cgctactacg actccggcga acgaatatcc gtatcaatct
      421 ccggactcgc cggaggcgcc gcagatcgaa atgcctgata ctcccgcgta ctctccgctg
      481 gaggcccaga ttgcggctca gttccagcaa ggaaagcgcg gggcaagctg gttctattcg
      541 attgctgggt tctcgctgat caattcgatc atcgtcctct tcggaggagg gacctatttt
      601 gtcattggcc tgggggtcac ggtgatcacc gaccactttg ccgtcgccgc cgcccagcag
      661 tcgcccgacg cggccgttct cgtcaaaggg ggcgcgtttg cctttgacct gtttgttgcc
      721 gccgccctct gcgcctttgg ctggctctcg gtaaagcgct ggcaaccggt gttcatcctg
      781 ggaatgacgc tctacctcct cgacggcctg ctgttcctga tgttcggccg gatgatggcc
      841 gtggcgttcc acgcctatgg cctgtactgc atgtggagcg gctttcaggc gtatcgccgg
      901 ctggcggacc tggaacggca actgatggac ccgcttcacc cggcggggat gctcaatccg
      961 catcaagcgg caccgctcga gggaacgtaa ccgcaggcat tactccaact cgacaatatc
     1021 ccccagcacg ccgcgcggga tgcagacagt gagcaccgtc aacttgccga cggccttgtg
     1081 cttgacccct cgcggaacat acacaaccac gccgggatga agatcaattt cgtcatcgtc
     1141 cagaaccatc gtgccggtgc cggcgataat gtagtaaaac tcgtcggtgc gggcatggta
     1201 gtggagcttg gcgtgatcaa tctcgacgtg atgcacttcc gcagccgcct gatccttctc
     1261 ttccacgagg gggcggatct ggccgcaagt ttcccgccaa ggctcgactt cggcgggatc
     1321 gcggcggaga tatttggttg cggtcgcggt cattggctgg ctccgaaacg agggcgaagt
     1381 tgatgcctca ttctctcatc agttggcccg aattgccaga caactctagg cccttccaag
     1441 cgagtctgcg ataatagccc catgctcaac ctcggcaggc aaatcgcgaa agtctgcggc
     1501 cgcaccacgc ggcgagagtt gctccgcgcg ggcggactgg ctgctgcggg gctgtcgctg
     1561 gccgatttgc tgcggctgga agccagggga gcgtcggaaa acgaacccaa ggcgaagtcg
     1621 gtgatcctgc tgtggctctg ggggggaccg agtcatctcg acacgttcga catgaagcct
     1681 aaagccccgg tcgattaccg cgggccttat gcgccggtcc cgacgaaaat ccagggcatc
     1741 cagatctgcg aattgtttcc gttgttggcg gctcgggccg ataggtactc catcgtgagg
     1801 tcgatgcatc acacctcgaa cgatcacggc atcgggggca ctatcggcct gaccggttcg
     1861 gacagcgggg ccatcagcct tagcgggcag gtcctctccg gccaggtcaa agcgacgcac
     1921 ggctccgtgg tctcccgggt cctcggattc tcgcccgaga tgccgcggtt tgtcacgatg
     1981 ggaggaacgc tgcatcaagg caaacggtcg atcgccggcg agagcgctgg accgctcggc
     2041 gcgctttacg atccgttccg tctcgactac gacgtgcagc aaggagtcaa gattccttct
     2101 ctcgatctga ttgacggcaa ctcggcgagc ggcctcgaat cccgtgaagg tctccgcaag
     2161 cgtatggacg gtctcttgcg agacttggaa tcttcttctg ct
//
LOCUS       DASUGB010001083         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_814957, whole genome shotgun sequence.
ACCESSION   DASUGB010001083 DASUGB010000000
VERSION     DASUGB010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814957"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFV87_20545"
     CDS             complement(<1..1148)
                     /locus_tag="VFV87_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5106226.1"
                     /translation="MKRLVLRRAAGICVAALTLAVFSGRLQAVEPQLGSIAPYGVQRG
                     TDVELVFGGGRLADAKELLFYSPGFTVKSLEAADNAVKAQLTVAPDCRLGIHAVRLRT
                     ATGVSNLRTFTVGTLPEVKEVEPNNDFLQPQVIQLDVTVSGIVQNEDIDQFVVELKKG
                     QRLSAELEGLRLGNTFFDPYVAILNAARFELDRSDDAALLSQDCLCSIVAPEDGKYIV
                     QIRESSFGGNGACTYRLHVGTFPRPTAAFPPGGKPGETLQVRWIGDAAGDFTQEITLP
                     TDGSIETNVVAQDASGQAPSPNVLRVIDLANSNEVEPNDAVPQATPAAAAPLAMNGIL
                     EKEGDIDFFKFSAKKGQQLDVRVYARNPLRSPLDSVLVVYNAQGGGIA"
     gene            complement(1312..>2405)
                     /locus_tag="VFV87_20550"
     CDS             complement(1312..>2405)
                     /locus_tag="VFV87_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106227.1"
                     /translation="YAPIEYRGEMGTVKTKTGEVFSETLPKLASVADKLAVIRSMTHG
                     EAAHERGTHNMFTGYRPSPALIFPSMGSVISHEYGPRNNLPPYVCIPNMPTEYAGNGY
                     LSSSFAPFSLGSDPAQNGFKVQDLALPGGVDDARFTRRRTALEAVNDYFAKKDKSDNV
                     GAMNTFYERAYSLISSQQAREAFNIDAEPANIRDEYGRNVAGQRLLMARRLVAAGVRF
                     VTLTYGGWDMHNQITAGFRNQMPALDQALSTLINDLDRNGLLATTMIMVSSEFGRTPK
                     INNTAGRDHWPKVFSVMLAGGGIKGGVIYGTSNATASEPDMDAVGPEDLATTVYRQLG
                     IVADKELMAPGDRPIEICDGGKVIKGLLA"
BASE COUNT          432 a          729 c          771 g          473 t
ORIGIN      
        1 ctggcaatcc cgccaccttg agcgttatag acgaccagca ccgaatcgag gggcgagcga
       61 agcggattgc gggcatagac gcgaacgtcg agctgctgcc ccttctttgc cgaaaacttg
      121 aagaagtcga tatcgccttc cttttcgagg atgccgttca tcgccagtgg ggcagccgcg
      181 gcgggtgtcg cctgggggac tgcatcgttc ggctcgactt cgttggaatt ggccaggtcg
      241 atcacccgca gaacattggg cgagggagcc tggccggaag catcctgggc cacgacgttg
      301 gtctcgatcg atccatccgt gggaagcgtg atctcttgtg tgaaatcacc tgccgcatcg
      361 ccgatccagc ggacttgcag cgtttcgccc ggcttgccgc ccggcgggaa cgccgcggtt
      421 ggccgcggga acgtgccgac gtgcagacga tacgtgcaag cgccgttgcc gccgaacgac
      481 gattcgcgaa tctgaacgat gtatttgccg tcctcgggcg cgacaatcga gcacaggcag
      541 tcctggctga gcaatgccgc gtcgtcgctg cggtcgagtt cgaaccgggc cgcattgagg
      601 attgccacgt agggatcaaa aaaggtgttt cccagccgca agccttccaa ctcggccgag
      661 agccgctggc ccttcttcag ttcgacaacg aactggtcga tgtcttcgtt ctgcacgatc
      721 ccgctgacgg tgacgtcaag ctgaatcacc tgcggctgga ggaaatcgtt attgggctcg
      781 acctccttga cctccggcag agtgccgacg gtgaatgtcc gcaaattgct cacgccggtc
      841 gctgtccgca gccggaccgc gtgaatcccc aggcggcaat cgggggctac cgtgagctgc
      901 gcctttacgg cattgtcggc tgcttccagg cttttgaccg tgaatcccgg cgaatagaag
      961 agcagttcct tcgcatccgc caggcggccg ccgccgaaga ccaattccac atccgtgccc
     1021 cgctgcacgc catacggcgc gatgctgccg agctgcggct caacagcttg caggcggccg
     1081 ctaaaaacag ccagagtcag tgcggcaaca cagatgccgg cggcgcggcg caggaccaag
     1141 cgtttcatga cagcctcatc tcaatttcca tccgacccca aggcgggcac acgatgtcgt
     1201 gcgagggtgg atcaggcggg tgcggcaatt cgtaggatgg acctcctggt ccacccaaat
     1261 tcaaagcagg acggaccacg aggtccgtcc tgcaaggttg attgtactta cctaggccag
     1321 caggcccttg atgaccttgc cgccgtcgca aatttcgatc gggcgatcgc ccggagccat
     1381 caactccttg tcggcgacga tgcccagctg gcgatacacg gtggtggcca agtcttcggg
     1441 gccgacggca tccatgtccg gctcggaggc ggtcgcgttc gatgtgccgt aaattacgcc
     1501 gcctttgatg ccgccgccgg cgagcataac gctgaacacc ttcggccagt ggtcgcggcc
     1561 ggcggtgttg ttgatcttgg gcgtgcggcc gaactcgctg gaaaccatga tcatcgttgt
     1621 cgccaagagg ccgttgcggt ccaggtcgtt gatcagggtc gaaagggcct gatccagggc
     1681 cggcatctgg ttgcggaacc cggcagtgat ctggttgtgc atgtcccagc cgccataggt
     1741 cagcgttaca aatcggacgc cagcagcgac caggcgacga gccatcaaga gccgctggcc
     1801 agcgacattg cggccgtatt catcgcggat gttggccggc tcggcgtcga tattgaacgc
     1861 ctcgcgggcc tgctgcgagc tgatcaggct gtaggcccgt tcgtagaagg tgttcatcgc
     1921 cccgacgttg tccgacttgt ccttcttggc gaagtagtcg ttcaccgctt ccagggcagt
     1981 tcggcggcgg gtaaagcggg cgtcatcgac gccgcccggc agggcgaggt cctgcacctt
     2041 aaagccgttc tgggccggat cggagcccag gctgaacggg gcgaacgacg agctaaggta
     2101 gccattgccg gcgtattcgg tcggcatatt aggaatgcag acgtacggtg gcagattgtt
     2161 ccgcgggccg tattcgtggc tgatgacgct tcccatgctg gggaaaatca gggccgggct
     2221 gggtcgatag cccgtgaaca tgttgtgcgt gcctcgctcg tgggccgctt caccgtgggt
     2281 catcgagcgg atgaccgcca gcttgtcggc caccgacgcc agtttcggca gcgtttcgct
     2341 gaagacttcg ccggtcttgg tcttcaccgt ccccatttcg ccgcgatatt cgatcggggc
     2401 gtagg
//
LOCUS       DASUGB010001084         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2065971, whole genome shotgun sequence.
ACCESSION   DASUGB010001084 DASUGB010000000
VERSION     DASUGB010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065971"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFV87_20555"
     CDS             <1..581
                     /locus_tag="VFV87_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5106228.1"
                     /translation="YQRQRQLTLGDLLLENRIVFLQGEIYTGNANELVMKLLYLQSEN
                     RRKDIHFYLNSPGGDVAATLAIYDTMQILSCPVATYCVGQAASGAAVLLAGGTKGKRY
                     ALPHSRIMLHQPYGGVQGQVSDIEIQANEILRNREMINEILAKHTSQPIERIAKDTDR
                     DFFLGAEDAKKYGLVDDVLQKMPGPGGEDDKD"
     gene            673..1284
                     /gene="clpP"
                     /locus_tag="VFV87_20560"
     CDS             673..1284
                     /gene="clpP"
                     /locus_tag="VFV87_20560"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX5106229.1"
                     /translation="MPLVPYVVEKSGREERVYDIYSRLLKDRIMFLGSQVNDEVANLL
                     VAQMLFLQSEDPKGDIHLYINSPGGSVSAGMAIYDTMQFVTCDVATYCVGQAASMGAV
                     LLTAGAPGKRFALPNARIMIHQPLAGMQGTAEEIMIHAKEFKRIKQKMNEILIKHTGH
                     PLEKIEQDTDRDRFMSADEACEYKLVDKVIERMQTLPQAKSSE"
     gene            1360..1701
                     /locus_tag="VFV87_20565"
     CDS             1360..1701
                     /locus_tag="VFV87_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106230.1"
                     /translation="MLPSPHAEPERYQGRPLLIVLENYVLDCIGKLPPDSSEKLSKLM
                     QDVLGGGPDWRQTLRETLHLDPALDGELQRLWTTNAQLAKQDGIELHPVQFAKMVVDE
                     NFADLVGKPCE"
     gene            1722..3155
                     /locus_tag="VFV87_20570"
     CDS             1722..3155
                     /locus_tag="VFV87_20570"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5106231.1"
                     /translation="MPRLLFASLATLVFSVCTANAAEGPYLATGIKIGEVTSDSAIVW
                     ARLTAIEKPAQPPLGQAQPAKAADAEISGLAGAVPGMAGNVSLEISTSADFTDAKRFE
                     SARVDAATDFTHQWSVANLKPATKYFVRVTAAAKEGQPTAKLEGSFTTASAASEWQDV
                     RFAVMSCQSHRDRDELEGFHIYPAMQKANIHFYVATGDNVYYDSDPPLADTVALARFH
                     WNRMHGLPGLVEFHRHVPGYWEKDDHDSFRNDDWPPLGRNADRSMELTGDLNFFKGLK
                     IFREQVPIGEKTYRTFRWGKSLQIWLTEGRDFRSPNNMPDGPDKTIWGQEQLEWLKSS
                     LLASDADFKVLISPTPVVGPDRKNKADNHSNATFGAEGNAFRKWIQDNKLTGNFFTCC
                     GDRHWQYHSVDPATGLNEFSAGAASDKHAGGSPGLDPEFHKFHRVKGGFITVSVARPD
                     GVPKITFRHHDVNGEVVYEYAATQERG"
     gene            3162..>6518
                     /locus_tag="VFV87_20575"
     CDS             3162..>6518
                     /locus_tag="VFV87_20575"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5106232.1"
                     /translation="MTDDRWQFWLDVGGTFTDCIARQPDGTLLRRKVLSSAVTKGQAE
                     SDPATRTLHDATRQEPPGFWIGYRVRLSGSEVAIADSRPGEIQVTTPLPERHGGHSLQ
                     NGGRSLQHYELVSPEDAPLLAIRLFLGLRLDEPIPPCVVRLGTTRGTNALLTRRGAKT
                     ALATTKGHADVLRIGYQNRPKLFELAIKKPPPLFAEVAEIDERIAADGSILLAPEPQQ
                     VQAELERLKAAGIESLAVCLLNAYANCVHEQLVGQIARDLGLTEISLSHQVSPLMKIV
                     PRGDTTVVDAYLNPVLQQYVARLAAALPGSDVRLMTSAGGLASAGNFTGKDSILSGPA
                     GGVVGFSKVAQAAGFERAIGFDMGGTSTDVSRFDGQYEYEYETEKAGVRIVAPMLAIE
                     TVAAGGGSICKWDGVKLVVGPDSAGADPGPACYGRGGPLTITDCNFYLGRILPEHFPF
                     PLDRDAVEARLAEVAEAVRAGSSRNSMEGDSSRNATEGVPYSACALAEGFIRIANTHM
                     AQAIRSISIAKGYDVRDYVLVAFGAAAPQHACGVASELGIRQILIHPDAGVLSALGIG
                     LADIVKHRTAPIYRPCDRKGMASARRAVLELTQQVMDDLRAEGFHTGRAVIHHVLDLR
                     YVGTDVPISIHFSDPRTNVIARFAEEHRRLYGYTHPSRPLEIVAIRVSATAYSESTLP
                     ESRPVSPVRVVPQAMLNLHHAGRLLNTELYFREQLSAGGRMEGPALIVEPLTTTIIDP
                     GWSAEVLTGGELLLTADSSPPLLLSPSPTLASAELDPILLEVTGNHFTAIATQMGITL
                     RNTAMSVNVKERLDFSCAIFTPEGDLVVNAPHIPVHLGAMSETVKQIIADNPAIQAGD
                     VFVTNDPYRGGSHLPDVTVVTPVFCEGRPESPTIRNPQSAIRNPAPAFFTASRAHHAE
                     IGGITPGSVPPFSKNLAEEGVLIRNFKLLDAGISRFDELRSLLSAGPYPSRSPETNLV
                     DITAQLAANHQGAEDLRRMIQQYSLPVVEAYMRHIQDAAERKMRQAFAKMTPGRREFV
                     DHLDDGTPICVAVTIHRETATIDFTGTGPVSKGNLNANRAIVSAAVMYVLRLLVGEDI
                     PLNQGVLAPVTIVLPECILNPPERERPEDCP"
BASE COUNT         1294 a         2112 c         1989 g         1123 t
ORIGIN      
        1 cataccagcg gcagcggcag cttacgctgg gggatttgct gctggagaac cggatcgtct
       61 ttcttcaagg ggaaatctac accgggaatg ccaacgaatt ggtgatgaag ctcctgtatt
      121 tgcagagcga gaatcgccgc aaggacattc acttttactt gaattcgccc ggcggcgatg
      181 tcgccgccac gctggccatt tacgacacga tgcagatcct gagctgcccg gtggcgacgt
      241 actgcgtcgg ccaggcggcc agcggcgctg cggtgctgct cgccggcgga accaagggca
      301 agcgatacgc cctgccgcac tcgcgaatca tgctccacca gccctacggc ggcgtgcagg
      361 gacaggtgag cgacatcgaa atccaggcca acgaaatcct ccgcaaccgt gagatgatca
      421 acgaaatcct cgccaaacac acctcccagc cgatcgaacg gatcgccaaa gacaccgacc
      481 gcgacttttt cctcggcgcc gaggacgcca agaagtacgg cctggtcgat gatgtactgc
      541 agaagatgcc gggacccggc ggcgaagatg acaaggacta gcaggctcgt cccgggccaa
      601 tttgcaattt gcggtttgca attgcaaatt ttcgattgaa aagtgcaaat tgcaaaatga
      661 ggaagaccgt acatgcctct cgttccctac gtcgtcgaaa aaagcggccg cgaagagcgg
      721 gtctatgaca tctacagccg cctgctcaag gaccgcatca tgttcctggg ctcgcaggtg
      781 aacgacgaag tcgcgaacct gctcgtggcc cagatgctat tcttgcaatc cgaagaccct
      841 aagggcgaca tccatctcta catcaattcg cccggcggca gcgtgagcgc cggcatggcg
      901 atctacgaca cgatgcagtt cgtcacttgc gacgtagcga cctactgcgt cggccaggca
      961 gcctcgatgg gcgcggtgct cctcacggcg ggagcgccgg gcaagcggtt cgccctgccc
     1021 aacgcgcgaa tcatgatcca ccagcccctg gccggcatgc agggaaccgc cgaggagatc
     1081 atgatccacg ccaaggagtt caagcggatc aagcagaaga tgaacgagat cctgatcaag
     1141 cacactggtc atccgctgga gaagatcgaa caggacaccg accgcgaccg cttcatgtcc
     1201 gccgacgaag cctgcgaata caagctggtc gataaggtca tcgaacggat gcagaccctc
     1261 ccgcaggcca agtccagcga gtagcgctcg taggacgggt ttctaacccg tctccgccgc
     1321 cttctaggac gggttggaaa cccgtcctac aatggcgaga tgctcccctc cccccacgcc
     1381 gagcccgagc gctatcaagg ccgcccgctc ttgatcgtgc tcgagaacta cgttctcgat
     1441 tgcatcggca agctcccgcc cgacagcagc gagaaactct ccaagctgat gcaggacgtc
     1501 ctgggcggtg gcccggactg gaggcagacg ctccgcgaga cgctgcacct cgatccggct
     1561 cttgacggcg aattgcaaag gctgtggacg acgaatgccc aactcgcgaa gcaggacggt
     1621 atcgaattgc atcccgtcca gttcgcaaag atggtcgtcg acgagaattt cgccgacctg
     1681 gtcggcaaac cctgtgaata gctccaccga ggcgcaacac catgccacgc ttactcttcg
     1741 cgtccctcgc gacccttgtc ttctccgtct gcaccgccaa tgccgcggaa gggccctatc
     1801 tcgcgaccgg aatcaagatc ggcgaggtga cgagcgattc ggcaatcgtc tgggcccggc
     1861 tgacggccat cgaaaagccc gcgcaaccgc cgctcggcca ggctcagcca gcgaaagctg
     1921 ccgatgcgga gatttccggc ctggccggcg cggtgccggg aatggcgggg aacgtttcgc
     1981 tcgaaatctc gaccagtgcc gattttaccg atgccaagcg gttcgaatcg gcccgcgtgg
     2041 acgccgcaac cgacttcacg caccagtggt ccgtcgccaa tctgaagccg gcaacgaagt
     2101 acttcgtccg cgtcaccgcc gccgcgaagg aagggcagcc gacagcgaag ctggagggga
     2161 gcttcacgac cgcgtcggcg gccagcgagt ggcaagacgt gcggtttgcc gtcatgtcgt
     2221 gccagtcgca ccgcgaccgc gacgagttgg agggctttca catttatcca gcgatgcaga
     2281 aggcgaacat ccacttctac gtggcgactg gcgacaacgt ctattacgac agcgatccgc
     2341 cgctggccga tacggtcgcc ctggcccgct ttcactggaa ccggatgcac ggcttgcccg
     2401 gcctggtcga attccaccgc cacgtccccg gctactggga gaaggacgac cacgacagct
     2461 ttcggaacga cgattggccg ccgctgggcc gcaatgccga tcgctcgatg gaactcaccg
     2521 gcgacttgaa cttcttcaag gggctcaaaa tcttccgcga gcaagtgccg ataggcgaga
     2581 agacctatcg cacattccgc tggggaaaaa gcctgcaaat ctggctgacg gagggtcggg
     2641 atttccgctc gcccaacaac atgcccgacg ggccggacaa gacgatctgg ggccaggagc
     2701 agcttgagtg gctgaaaagc agtctgctgg ccagcgacgc cgatttcaag gtgctcatca
     2761 gccccacgcc cgtcgtcggc cccgaccgca agaacaaagc cgacaatcat tccaacgcca
     2821 cgttcggcgc cgagggcaac gccttccgga agtggatcca ggacaacaag ctcaccggca
     2881 acttctttac ctgctgcggc gaccggcatt ggcagtacca ctcggtcgat ccggcgaccg
     2941 ggctgaatga gttctcggcc ggcgcggcca gcgacaagca cgccggtggc tcacccggcc
     3001 tcgatcccga gttccacaag ttccaccgcg tgaaaggggg cttcatcacc gtcagcgtcg
     3061 cgcggccgga tggcgtgccg aagatcacgt ttcgtcatca cgacgtgaac ggagaagtcg
     3121 tttacgaata cgccgcgacg caagagcgcg gctaagagcg tatgacggac gaccgctggc
     3181 aattctggct cgacgttggc gggacgttca ccgactgcat cgcccgccaa ccggacggca
     3241 cgctcctgcg gcggaaagtg ctcagttcgg ccgtcacgaa gggacaggct gaatccgatc
     3301 cggcgacgcg aacgctgcac gacgccacgc ggcaggaacc gcctggattc tggatcggat
     3361 accgcgtgcg gctttcgggc agcgaggtcg ccatcgccga ttcgcggcca ggcgagattc
     3421 aggttacaac gccgctcccg gaacgccacg gagggcattc cctacagaac ggaggacgtt
     3481 ccctacagca ttatgagctg gtctcgcccg aggatgcgcc gcttctggcc atccgtctct
     3541 ttctgggctt gcggctggat gagccgattc cgccgtgtgt cgtgcgcttg ggcacgacgc
     3601 gcgggacgaa cgccctgctg acgcggcgcg gagccaaaac ggcactcgcc acgaccaagg
     3661 ggcacgccga cgtcctccgc atcggctatc agaaccggcc caagctcttc gagctggcga
     3721 tcaagaagcc gccgccgctc tttgccgaag tggcggaaat cgatgagcgg atcgccgccg
     3781 acggctcgat cctgctagcg ccagagccgc agcaagtaca ggcggagctg gagcgcctca
     3841 aagccgccgg cattgagtcg ctggccgttt gcctgctgaa tgcctacgcc aactgcgttc
     3901 acgagcagct cgtcggccag atcgcccgcg acctgggcct cacggaaatc agcctctcgc
     3961 accaggtctc gccgctgatg aagatcgtgc cccggggcga tacgacggtc gtcgatgcgt
     4021 atctgaatcc ggtcctgcag caatacgtcg cccgcttggc cgcggcgctt cccggcagcg
     4081 acgtgcgatt gatgacctcc gcgggcggac tcgccagcgc ggggaatttc accggcaagg
     4141 acagcattct ctccggcccg gccggcggcg tcgtgggctt ctcgaaagtc gcccaggcag
     4201 cggggtttga aagggccatc ggctttgaca tggggggcac gagcaccgat gtctcgcggt
     4261 ttgacggcca gtacgaatac gaatacgaga ccgaaaaggc cggcgtgcgg atcgtcgcgc
     4321 cgatgctggc gatcgaaacg gtcgccgcgg gcggcggctc gatctgcaaa tgggacggcg
     4381 tgaagctcgt cgtcggtccg gacagcgctg gcgccgatcc cggccccgcc tgctacggcc
     4441 gcggcggccc gctgacgatc accgactgca atttctacct cggccgcatc ctgcccgagc
     4501 attttccgtt tccgctcgat cgcgatgcgg tcgaagcgcg gctggccgaa gtcgccgagg
     4561 cggtgcgtgc cggcagttcc cggaactcca tggagggtga cagttcccgg aacgccacgg
     4621 agggcgttcc ctacagcgcg tgtgccctcg ccgaaggctt tatccggatc gccaatacgc
     4681 acatggccca ggcaatccgc agcatttcga tcgccaaagg ctacgacgtg cgcgactacg
     4741 tcctcgtcgc cttcggcgcc gcagcgccgc agcacgcctg cggcgtcgca agcgaactgg
     4801 gaatcaggca gattctgatt catcccgacg ccggcgtact cagcgcactg gggatcggcc
     4861 tggcggacat cgtgaagcat cgcaccgcac cgatttaccg gccctgcgat cgcaaaggga
     4921 tggcctccgc gcgtcgtgcc gttctcgaac tcacgcagca agttatggac gacctccgtg
     4981 ccgagggatt ccacactgga cgggccgtta tccaccacgt tctcgacttg cgctatgttg
     5041 gaaccgacgt tccaatcagc atccacttca gcgatccacg gaccaacgtc atcgcgcggt
     5101 ttgccgaaga gcaccgacgc ctctacggtt acacgcatcc gtcacggccg ctggagattg
     5161 tagcgattcg cgtttcagct acggcctact cggaaagcac gttgccggaa tcgcggcccg
     5221 tttctcccgt tcgcgttgtt ccccaggcaa tgctcaacct tcatcacgca ggccgcctcc
     5281 tgaatacgga gctgtacttt cgcgagcaac tttctgcggg cggccgaatg gaaggtccgg
     5341 ctctcattgt cgagccattg accacaacga ttatcgatcc cggctggtcg gccgaagtcc
     5401 tcacgggcgg cgaactcctg ctgacagccg attcgtcgcc ccctctcctt ctctccccct
     5461 ctcccactct tgcctcagcc gagctcgacc cgatcctgct cgaagtgacc ggcaaccact
     5521 tcaccgccat cgccacgcaa atgggaatca cgctccgcaa tacggcgatg agcgtgaacg
     5581 tgaaggagcg gctggacttt agctgtgcga tcttcacgcc ggagggggac ctcgttgtca
     5641 atgcgccgca cattccggtc cacctcggcg cgatgagcga gacggtgaag cagatcatcg
     5701 ccgacaatcc ggccatccag gccggcgatg tgttcgttac gaacgatccg tatcggggtg
     5761 gatcgcatct gccggacgtg acggtggtca ccccggtgtt ctgcgaaggt cgacccgaaa
     5821 gtccgacaat ccgcaatccc caatccgcaa tccgcaatcc cgccccggct ttcttcaccg
     5881 ccagccgggc ccatcatgcg gagatcggcg gcatcacgcc gggttcggtg ccaccgttct
     5941 cgaagaacct ggcggaggag ggcgtgctga tccgcaactt caagctgctg gatgccggca
     6001 tcagtcgctt tgatgagctg cggtcgctct tgtcggcggg cccctatccg agtcgttctc
     6061 cggaaacgaa ccttgtcgat atcacggcgc agctcgcggc caaccaccag ggcgctgaag
     6121 acctgcggcg gatgatccag caatactcgc tgccggtcgt ggaagcctac atgcggcaca
     6181 tccaggacgc ggccgagcgg aaaatgcggc aggcgttcgc gaagatgacg ccggggcggc
     6241 gggagttcgt cgatcatttg gacgatggga caccgatttg cgtggccgtg acgatccata
     6301 gagaaacggc gacgattgat ttcacaggga cggggccggt gtcgaagggg aacctgaacg
     6361 cgaaccgggc cattgtgtcg gctgcggtga tgtatgtgct gcggctcttg gtgggtgagg
     6421 atattccgct gaatcagggc gtgctcgcgc ccgtgacgat cgtgctgcca gagtgcattc
     6481 tcaatccacc cgagcgggaa cggccggaag attgcccg
//
LOCUS       DASUGB010001085         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1231728, whole genome shotgun sequence.
ACCESSION   DASUGB010001085 DASUGB010000000
VERSION     DASUGB010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231728"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1399)
                     /gene="bioA"
                     /locus_tag="VFV87_20580"
     CDS             complement(47..1399)
                     /gene="bioA"
                     /locus_tag="VFV87_20580"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX5106233.1"
                     /translation="MPEPSLDDLKLWDRTHVWHPFSQMAEYEPLLVRSAHGCTLVDVN
                     GHEYLDGVSSLWCNLHGHRHPQLDAAIRRQLEEVAHVTLLGMSHPTTVKLARKLVEIA
                     PAKLDRVFFSDDGATAVEVALKMAFQYWRQCPNPQPRKTKYLAFDNAYHGDTLGSVSV
                     GGVARFHQMFRPLLFDVVRLPTPDMYRLPAGVTCETACQHYLAQLETALCQHDEELAA
                     VVIEPLVQAAAGMVLHPPGFLRGVRELADKHHVLLIADEVAVGMGRTGRMFACEHEEV
                     APDFLCLAKGLTGGYLPLAATLTTTEIWNAFLGRYEDSKSFFHGHTYGGNPLGAAVAL
                     ATLEVFEEEETLQNLQPKIARLGKHLQRIAKLPHVGDVRQCGLIAGIELVRDRATKDP
                     YPWVEKRGIRVCRHALTEGVWLRPLGNVVVIMPPLAISLDELDRICGAVEHGIEVAIR
                     "
     gene            complement(1410..1697)
                     /locus_tag="VFV87_20585"
     CDS             complement(1410..1697)
                     /locus_tag="VFV87_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HEX5106234.1"
                     /translation="MAGKIEGKVVAYGETGNLVTDIAADALQSAPRDQSVTITCDEHE
                     TVGIYPADHPEEACTLMAILGSGGCLELTIVGDSAKMMLGVSLGERVVVKW"
     gene            complement(1810..2649)
                     /locus_tag="VFV87_20590"
     CDS             complement(1810..2649)
                     /locus_tag="VFV87_20590"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX5106235.1"
                     /translation="MRPLITLTTDFGSGSPYVAQMKGVILSLCREVDLVDISHAVGPQ
                     NIREGAVVLADATPRFPPGTIHVAVVDPGVGTSRPILYAEIGQQRYVAPDNGLLSLLA
                     SRQRPQRIRALENDSFWLPGTSHTFHGRDIMAPVAAHLALGVNPDELGPLAAGLHLLP
                     WPQPQRTGDEVSGEVLYVDSFGNVITNLSRDDVRAAGNPARLAIECAGRTIRGLVPTY
                     AAAMPQELVALFDSQGRLELAVVQGNAARELEVEPGAAVTVAEANEWEKPGRSAGDEW
                     NRP"
     gene            2726..>3236
                     /locus_tag="VFV87_20595"
     CDS             2726..>3236
                     /locus_tag="VFV87_20595"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5106236.1"
                     /translation="MNPALNGLPQSQTAPGEMIPPLEPGDRLTRDEFERRYAAMPHLK
                     KAELIEGVVYMPSPVRVYRHGKPHADVIGWMFVYRAATPGVDGGDNTTARLDLENEPQ
                     PDALLFVEPARGGQVKISEDDYIEGAPELVAEVASSSVSFDLNSKLHVYRRCGVKEYV
                     VWRVLDKAID"
BASE COUNT          570 a         1010 c         1078 g          578 t
ORIGIN      
        1 catcgtagcc cgtagcgcaa gcgagggaaa cgggcgcgag tatcagtcaa cgtatagcga
       61 cttcgatgcc gtgttcaacc gctccgcaga tgcggtcgag ctcgtcgagt gagattgcga
      121 gcggaggcat gatgacgacg acgttgccca gcgggcgcag ccagacacct tcggtcagcg
      181 cgtggcggca gacgcggatg ccccgttttt cgacccaggg ataggggtct ttcgtcgccc
      241 gatcgcggac gagctcgatg ccggcgatca ggccgcattg gcggacgtcg ccgacgtgcg
      301 gcagcttggc gatgcgctgc agatgcttgc cgaggcgggc gattttgggc tgaaggttct
      361 gaagcgtttc ctcttcctcg aaaacttcca gcgtcgccag cgcgacggct gcgccgagcg
      421 gattgccgcc gtaggtgtgg ccgtggaaga acgatttcga gtcttcgtag cgcccgagga
      481 aggcgttcca gatttcggtg gtagtcagcg tcgccgccag cggcagatag ccgccggtga
      541 ggcccttggc gaggcagagg aaatcgggag cgacttcctc gtgctcgcag gcgaacatcc
      601 ggccggtgcg gcccatgccg acggcgactt cgtcggcgat caggagcacg tggtgcttgt
      661 ccgccagctc gcgaacaccg cggaggaagc ccggcggatg cagcaccatt cccgcggccg
      721 cctgcaccag cggctcgatg acgacggcgg ccagttcctc gtcgtgctgg cagagcgcgg
      781 tttcgagctg ggcgaggtaa tgctggcagg ccgtctcgca cgtcacgccc gcgggaaggc
      841 gatacatgtc gggcgtcggc aggcggacga catcgaacag cagcggccgg aacatctggt
      901 gaaaccgggc cacgccgccg acgctcacgc tgccgagcgt gtcgccgtga taggcgttgt
      961 cgaacgcgag gtatttcgtc ttccgcggct ggggatttgg gcattgccgc cagtattgaa
     1021 aggccatttt cagggcgact tccaccgcgg tggcgccgtc gtcgctgaag aagacgcggt
     1081 cgagctttgc gggagcgatt tctacgagct tccgcgccag cttgacggtc gtcgggtgcg
     1141 acattcccag cagcgtgacg tgggccactt cctcaagctg ccgccggatc gccgcgtcga
     1201 gctgcggatg gcggtgcccg tggaggttgc accacaggct gctcacgccg tccagatatt
     1261 catggccgtt gacgtccacc agcgtgcagc cgtgggcgct tcgaacgagc agcggctcgt
     1321 attcggccat ctggctgaag ggatgccaga cgtgcgtgcg gtcccaaagc ttcaggtcgt
     1381 caagagacgg ttcgggcatt ttcaggcgat caccacttga cgacgaccct ctcccccagc
     1441 gacacgccga gcatcatctt cgcgctgtcg ccgacgatcg tcagctccag gcagccgccg
     1501 ctgccgagga tcgccatcag cgtgcaagct tcctcggggt ggtcggcggg gtagatgccg
     1561 accgtttcgt gctcgtcgca ggtgatggtg acggattggt cgcgcggggc ggactgcagc
     1621 gcatcggcgg caatatcggt gacgagattg cccgtctcgc catacgccac gactttgcct
     1681 tcgatcttgc cggccaccac cgccctcccg ccagaattat tccggtcgca ttgtaaagcg
     1741 tggcagaacg cgggccaaga ctttaacggg ctcgtcgccg aatcttcatg ggatttccgc
     1801 ggccagccat cagggacggt tccattcatc gccggctgag cggccaggtt tctcccattc
     1861 gtttgcttca gcaactgtta ctgcggcgcc tggctcgact tccaattccc gcgccgcatt
     1921 gccctgcacg acggccaatt ccagccggcc ctgcgaatcg aacagagcca ccagctcttg
     1981 cggcatggcg gcggcgtacg tcggcaccag gccgcggatt gtccgcccgg cacactcgat
     2041 cgccagccgg gcgggattgc cggcagctcg cacgtcgtcg cgcgacaagt tggtaatcac
     2101 gttcccgaac gaatcgacat acagcacttc gccggacact tcgtcgccag ttcgctgcgg
     2161 ctgcggccag gggagcaggt gcaagccggc tgccagcggg ccgagttcgt ccgggttgac
     2221 gccgagggcc agatgcgccg ccaccggcgc catgatgtcg cggccgtgga aggtatgcga
     2281 agtgcctggc agccagaacg aatcgttctc cagcgcgcgg attcgctgcg gccgctggcg
     2341 gctggcgagc aggctgagca ggccgttgtc cggcgcgaca taccgctgct ggccgatctc
     2401 ggcatacaga atcggccggc tggtgccgac gcccgggtcg acgacggcga cgtgaattgt
     2461 tcccggcgga aaccgcggcg tcgcatcggc cagcacgacc gccccctcgc ggatgttctg
     2521 cggcccaacc gcgtgcgaga tatcgaccag gtcaacctcg cggcagagcg acaagatcac
     2581 tcccttcatc tgcgccacat acgggctgcc ggagccgaag tcggtggtga gagtgatgag
     2641 cgggcgcatg gaatcttagg catcgcgaga ggttgcccct taaggctaga atagaggagc
     2701 attgtgccag aggaaaggaa cgacgatgaa tccggctctg aacggcctgc cccaatcgca
     2761 aacggcgcca ggtgagatga ttcctccgct cgaacccggc gaccgcctga cgcgtgacga
     2821 attcgagcgc cgctacgccg cgatgccgca tctgaagaag gccgagctga tcgaaggagt
     2881 cgtctatatg ccatcgcctg ttcgcgtgta tcgtcatggc aagcctcatg ccgacgtcat
     2941 tggctggatg ttcgtttatc gcgccgcaac gccgggagtc gatggcggcg acaacacgac
     3001 cgctcgcctc gatctggaaa acgagccgca gccggacgcc ctgctgttcg tcgaaccggc
     3061 acgtggcggt caggtgaaga tcagcgaaga cgactacatc gaaggcgcgc cggaactcgt
     3121 tgccgaagtt gcgtccagca gcgtgagttt tgatctcaac tccaagttgc acgtctatcg
     3181 ccgctgcggc gtcaaggagt atgtggtgtg gcgcgtgctg gataaggcaa ttgact
//
LOCUS       DASUGB010001086         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1650885, whole genome shotgun sequence.
ACCESSION   DASUGB010001086 DASUGB010000000
VERSION     DASUGB010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650885"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFV87_20600"
     CDS             complement(<1..185)
                     /locus_tag="VFV87_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106237.1"
                     /translation="MFGNQSGLRERQAIIGAAAFIAILAISQFQPRPAHAEDTFGGAI
                     AVDELTEIRGGDDSASNS"
     gene            complement(430..801)
                     /locus_tag="VFV87_20605"
     CDS             complement(430..801)
                     /locus_tag="VFV87_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106238.1"
                     /translation="MKKLLVGVSALAIGLVASSGAWANPKNTFSNGNTQTVISLAIAG
                     GAAFSESFNVNQEAVSVQTLNGASTGGSLSVEVIPIFSGNAITGTQSGNSGVTMAQAN
                     SGNTSVQQQGVALSAVGTVNF"
     gene            1160..1705
                     /locus_tag="VFV87_20610"
     CDS             1160..1705
                     /locus_tag="VFV87_20610"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106239.1"
                     /translation="MLLCGATPWALAQDPFVAFGSRDYAAALAGFQALAAQGNPNAMN
                     NLGLMYANGLGVPRDDAVAVKWYGQAAAVGHIDAINNLGGMYEMGYGVPQNYASAAEK
                     YALAAKYGSSDAQYNLAALYESGKGVPNDALQAYIWYSLSAAQGDQDAAAGRGRMAEL
                     LDPTLRGQADTFVTAWKPGQN"
     gene            complement(1776..>2198)
                     /locus_tag="VFV87_20615"
     CDS             complement(1776..>2198)
                     /locus_tag="VFV87_20615"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5106240.1"
                     /translation="SLPPHIAGHRSIAVLWDIPDDRPEQQRKLAFIMDPDHRRNTEFD
                     VAALERIYGLTLTEARVAAFVGQNKSVAEIADQMGSTSHTVRTHLRHIFEKTGSERQL
                     DLVHLLLQSPLVLRLPQKRARPRPASAQPVAKPSPLRN"
BASE COUNT          405 a          653 c          705 g          435 t
ORIGIN      
        1 gaattgctgg cgctgtcgtc gccgccgcga atttccgtca gctcatcgac cgcaatggcg
       61 ccgccgaacg tgtcctcggc atgcgcgggc cgcggttgga actgactgat ggccagtatt
      121 gcgatgaacg cggcggcgcc aatgatggcc tgccgctcgc gaagcccact ctggttgccg
      181 aacataattc cgctccttgg cttgcgtgga gcggccggcg gtagttccgt atataccagc
      241 ccattattgc cctttggggg catgcaacaa caagcacagt atctcagccc aagccccctt
      301 ctacggcggt gacctgtcta acaccgccgt tggacaccaa aaggaggcaa gcctttgccg
      361 gggttaccgc cggcgccctc gcgcctcaac ctgtcagccc ttgttatgtg tgcccggact
      421 tagttgaagc tagaagttca ccgttccgac tgccgacagg gcgacgccct gctgctgcac
      481 ggaggtgttg cccgagttgg cttgcgccat cgtcacgccg gagttgccgc tctgggtgcc
      541 ggtgatcgcg ttgccggaga agatcggaat cacttcgacc gacagcgagc cgccggtgga
      601 ggcgccgttc agcgtctgca cggaaacggc ttcctgattc acgttgaacg attcactgaa
      661 cgctgcgccg cccgcgatgg ccaggctgat gaccgtctgg gtgttgccgt tcgagaaggt
      721 gttcttcggg ttggcccaag cgccagagga cgcaacgagg ccgatcgcca gcgcggacac
      781 gccgactaat agcttcttca tgactgtctc cttttggttt cgcgttgccg ccccccaatg
      841 ggtcggcaat gtggccctct gcccgcctca ggcagtgttg gtagtcgatc cagccacaac
      901 gcgatgacat catgaggagc caagagtgtc gtcatccaaa tgagtgaggc gacatggcga
      961 tttgatggat tattagatgc agcgtgtgtt tcatgtacga aagaaacggc gaccgtatga
     1021 aatacgaaat tctcttttag gaatcccgct gcgcggcgat gctgaatcat ccccgcgccg
     1081 gtgacatctt cgtggttcgg gaagatcgtc gaaggagagc tacatcatgc gatgtatgag
     1141 tcgggttcta gcctttctcg tgctgctgtg cggcgctacg ccatgggcgt tggcgcaaga
     1201 tccgtttgtc gcgttcggca gcagggatta cgcagcggcc ctggccggct ttcaagcgct
     1261 tgcggcacag ggcaatccga acgcgatgaa caatttgggc ctgatgtacg caaatgggtt
     1321 gggcgtgccg cgcgatgacg ccgtcgcggt gaaatggtac ggccaggccg ccgccgtcgg
     1381 ccacatcgat gccatcaaca atcttggcgg catgtacgaa atgggatacg gcgtgccgca
     1441 aaactatgcg tcggccgcgg agaaatatgc gctggcagcc aaatatggtt cgtcggacgc
     1501 gcagtacaat ctcgccgcgc tttacgaatc cggcaagggc gtgccgaacg atgccctgca
     1561 ggcctatatc tggtatagcc tgtcggcggc gcaaggcgac caggatgccg ctgcggggcg
     1621 gggccggatg gcagagctcc ttgatccgac cctgcgcgga caagccgata cgttcgtcac
     1681 ggcctggaag cccgggcaga attgaacgac agcgcggccg gcgcctcttg agggaacgaa
     1741 cgccgcaggt cgcgatgctt tcgctcgcgg acacgtcagt tgcgcagcgg tgacggcttg
     1801 gccacaggct gcgccgacgc cggtcttggc cgcgcgcgct tctgcggcag ccgcagaacc
     1861 agcggtgatt gcagcaggag atgaaccagg tccagctgcc gctccgaacc ggtcttctcg
     1921 aagatgtggc gcagatgcgt gcgcacggta tgagacgtgc tgcccatctg atcggcgatt
     1981 tccgcgacgc tcttgttctg gccgacaaac gccgcaacac gcgcttcggt cagcgtcaag
     2041 ccatagatgc gttccagcgc cgcgacatcg aactcggtgt tgcgccggtg atcggggtcc
     2101 atgatgaagg ccagcttgcg ttgctgctcg ggccggtcgt ctggaatatc ccagaggacg
     2161 gcgatcgatc gatggccggc aatgtgcggc ggcaggct
//
LOCUS       DASUGB010001087         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_538800, whole genome shotgun sequence.
ACCESSION   DASUGB010001087 DASUGB010000000
VERSION     DASUGB010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538800"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1480
                     /gene="gmd"
                     /locus_tag="VFV87_20620"
     CDS             371..1480
                     /gene="gmd"
                     /locus_tag="VFV87_20620"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5106241.1"
                     /translation="MSPMRALITGITGQDGSYLAEFLLEQGYEVHGMVRRLSADNWER
                     LGNVRDRVQLHSADLLDLTSLLNLLRDVRPQEVYNLAAQSFVPASWQQPILTGEVTGL
                     GVSRILEAILHVDRSIRFYQASSSEMFGTIPVEPQSEQTPFHPRSPYAVSKVFGHWIT
                     INFRESHGMFACSGILFNHESPRRGKEFVTRKITDGVARIKLGLQEKLMLGNLDARRD
                     WGFAGDYVRAMWLMLQQDKPDDYVVATGEKNSVREFAELAFAQVNLDWRDHVETDKRL
                     LRPAEVHSLRGDATKARDCLKWQPEVSFRELVGMMVTADLERVAAEIAQPAERRKETS
                     GELLEAGTRNLPAVAKYTFDRVAAPGQRGTPRRVA"
     gene            1529..2491
                     /locus_tag="VFV87_20625"
     CDS             1529..2491
                     /locus_tag="VFV87_20625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX5106242.1"
                     /translation="MLDLSTKRILVTGGNGFLGRHVVARLREIGCREIHAPRKAACDL
                     TDASEVRRLLELLQPQVVLHLAAQVGGIEANRRQPGTFFYSNLMMGANLIEGCRGLGG
                     LERFVLVGTICSYPKFAPVPFREEDLWNGYPEETNAPYGVAKKALLVQLQAYEQEFGF
                     PGVCVLPVNLYGPGDNFDLTTSHVIPALIRKFVEAQEQGQREATIWGTGRPTREFLYV
                     ADAARGICLAAEQLQGAEPVNLGTGHEITIADLAELIADKVGYRGRLVFDPARPDGQP
                     RRQLDVSRARQRLGFVAATSLDEGLERTVGWYRSQRSAGARAAA"
     gene            2691..3308
                     /locus_tag="VFV87_20630"
     CDS             2691..3308
                     /locus_tag="VFV87_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106243.1"
                     /translation="MNSSGSGMELQSNRWMQGNTSDRPSFDVLISRGLGGARRFQVFL
                     MTKGLLCLDKGPPRAGGGGGGDNNGAIVAGAMLGGLAGAAIAHALTSNSGGSCHAHEE
                     RYDLCDEDELLELAAARKKSFVAFYDDIGGVSLDVPGMWEKIFGDGRTVGWITLREKS
                     LGKVAMEVRDPAQMQVAIDTLPRRLGPRVQVNLQLDPRTTTYVRK"
     gene            3527..>3822
                     /locus_tag="VFV87_20635"
     CDS             3527..>3822
                     /locus_tag="VFV87_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5106244.1"
                     /translation="MPTHSPPLPKRRAFTLVELLVVIAIIGILVALLLPAVQAAREAG
                     RRSSCQNKIRQLAIGLHNHHDVFEKFPPGCEGTVLPVPNPAGNTTTIQGTSWIV"
BASE COUNT          696 a         1184 c         1248 g          694 t
ORIGIN      
        1 ttgcactcga cgagcttggg ccagccgagc ggtgcggcct gaaggtagaa cttggcgcgg
       61 cggaggacgc cttggccgat cgcggcgagg gcttgcataa ccggttcctt gtttcgcgcg
      121 cgaatcgcgc gtcacggcgg ccggaagcta gcccgcgccc gttgggccgt caatgtcgat
      181 cgcacgtaca gtaacccgaa gcgtcagcga gggcgtcagc aatgggcgct ggcttgccgg
      241 gagtccctcg ctgacgcttc gggttggtat gccccggcag gctggggttg gtgattgcgg
      301 cagccgctgc tataaaactc gcccgtctac cgcatggcat ccaagccgac gctagcaaag
      361 gagtgctgta atgtccccca tgcgcgcgct gatcaccggg attaccggac aggatggttc
      421 gtacctggcc gagttcctgc tggagcaggg ctacgaagtt cacggaatgg tccgccggct
      481 gagcgcggac aattgggagc gtctggggaa cgtgcgggac cgcgtgcaat tgcactcggc
      541 cgatttgctc gacctgacat cgctgctcaa tctgctccgc gacgtgcggc cgcaggaagt
      601 ttataacctg gcggcccaat cgtttgtgcc ggcgagctgg cagcagccga tcttgacggg
      661 cgaagtcacc ggcctgggcg tgtcgcgaat tttggaagcg attttgcacg tcgatcgcag
      721 catccgcttt taccaggcct ccagcagcga gatgttcggc acgattcccg tcgagccgca
      781 gagcgagcag acgccgttcc atccccgcag cccctacgcc gtgtcgaagg tcttcggtca
      841 ctggatcacg atcaacttcc gcgagagtca cggaatgttc gcttgctcgg gcatcttgtt
      901 caatcacgag tcgccgcggc ggggcaagga atttgtgact cgcaagatta ccgacggcgt
      961 cgcgcggatc aagctgggcc tgcaagagaa gctcatgctg ggcaatctcg acgcccgccg
     1021 cgattggggc tttgccggcg actacgtccg ggccatgtgg ctgatgctgc agcaggacaa
     1081 gccggacgac tacgtcgtgg cgaccggcga gaaaaattcg gttcgcgaat ttgccgagct
     1141 cgcgttcgcc caggtcaacc tcgactggcg cgatcacgtg gagaccgaca aacggctgct
     1201 gcggccggcg gaggttcact cgctgcgcgg cgatgcgacc aaggcccgcg attgcctcaa
     1261 gtggcagccg gaagtctcgt tccgagaact cgttggaatg atggtgacag ccgatctgga
     1321 gcgggtggca gcggagatcg cgcagccggc tgaacggcgc aaggaaacgt caggcgagct
     1381 cctcgaggcg ggaactcgca acttgcctgc ggtggcaaag tacacgttcg accgcgtggc
     1441 agcgccaggc cagcgcggca cgccgcgccg ggtcgcttag gctgccgctg gcggacgccg
     1501 ttcctgtcgc gctcttgtgg aagaaaatat gctcgatctg tcaaccaagc gaattctcgt
     1561 caccgggggc aatggctttc tgggacggca cgtcgtggcc cgcctgcggg agatcggctg
     1621 ccgcgagatt cacgcgccgc ggaaggcagc ctgcgattta acggacgcca gcgaagtccg
     1681 ccggttgctg gagctgctcc agccgcaggt ggtgctgcac ctggctgccc aggtcggcgg
     1741 catcgaggcc aaccgccgcc agccgggcac gttcttctac agcaacctga tgatgggggc
     1801 gaacttgatc gaaggctgcc gcggactcgg cggcctcgag cggttcgtcc tggtaggcac
     1861 catctgcagc tatccgaagt tcgccccagt gccgttccgc gaagaggacc tctggaacgg
     1921 ctatccggag gaaacaaacg ctccgtacgg tgtcgcgaag aaggcgctcc tcgtgcagct
     1981 tcaggcctac gagcaggaat tcggctttcc gggcgtgtgc gtgctgccgg tgaatctgta
     2041 cggcccgggc gacaacttcg acctgacgac ttcgcacgta attcccgccc tgattcgcaa
     2101 gtttgtcgag gcccaggagc aaggccaaag agaagcgacg atctggggca ccgggcggcc
     2161 gacgcgtgag tttctttacg ttgccgatgc cgcccgcggc atctgcctgg ctgccgagca
     2221 actccagggc gcggagccgg tcaacctggg cacgggacat gaaatcacga ttgcagatct
     2281 tgccgagttg attgccgaca aggtcggcta ccgaggccgg ctggtcttcg atcccgcgcg
     2341 gcccgatggt cagcctcgcc gccagctcga cgttagccga gcccgccagc gactgggatt
     2401 tgtggctgcg acttctttag atgagggcct ggagcgcacc gtcggctggt atcgcagcca
     2461 gcgctccgca ggcgcgcggg ccgccgcata agacgtgggg cagacattct tgtctgccac
     2521 aattgccccg tgtgtcgtca caagtcctga tgcggtaatc cgttgcgccc cggccgttat
     2581 tctctgacgc gtaacctagg cttcctctag gcggctgggc ggggatcgcg aagcaggcac
     2641 tgggcttggc gtcgcaatgg ccagccgccg ccaccacggg aaagcacgcc atgaactcat
     2701 ccggcagcgg catggaattg cagtccaatc ggtggatgca gggaaacacg tcggatcgcc
     2761 cctcgttcga tgtcctgatc agtcgcggat tgggcggagc gcgacggttc caggtattcc
     2821 tgatgaccaa gggactgctc tgcctggaca aaggcccgcc ccgcgccggc ggcggaggcg
     2881 gcggcgacaa caacggggcg atcgtcgcgg gggccatgct gggcggactg gcgggcgccg
     2941 ccattgccca cgccctcacg agcaactcgg gcggctcgtg ccacgctcac gaggagcggt
     3001 atgacttgtg cgatgaggac gagctgttgg aattggccgc agcccgcaag aagagctttg
     3061 tcgcgttcta cgacgatatc ggcggcgtct cgctcgacgt cccgggcatg tgggagaaaa
     3121 tcttcggcga cggccggacc gtgggctgga tcacgctccg cgaaaagtcg ctaggcaagg
     3181 tggcgatgga ggtccgcgat ccggcccaaa tgcaggtcgc gatcgacacg ctgccccgcc
     3241 gtcttggccc gcgagtgcag gtcaacctgc agctcgaccc ccgcacgaca acgtacgtgc
     3301 gaaagtaagc cgagaatcga cccccccccg tctgaagggc gttcgacggc agttttgaga
     3361 gttcgctagc cccaagatta tctagccagc ggtcattcag cgaatacttg cgccaatggc
     3421 ttggacaaga cgacatagtt agttacgctg caaaaacgga accgttttga tcgcttgggg
     3481 ttgaatagtg gtcgatccgg ttaattctct ccaaggtccc tcgtaaatgc ccacccactc
     3541 tccccccctc cccaagcgcc gcgcctttac tctcgtcgaa ctgctcgtgg tcatcgccat
     3601 cattggcatc ctggtagcgc tgttgctgcc ggcggttcaa gccgcccgcg aagcgggccg
     3661 ccgatcgagc tgccaaaaca agattcgtca gttggcaatc ggcctgcata accatcacga
     3721 cgtgtttgaa aagtttccac ccggatgcga ggggaccgtg ctgcccgttc ccaaccccgc
     3781 gggcaatacc accacgattc aggggaccag ttggatcgtc ca
//
LOCUS       DASUGB010001088         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_955322, whole genome shotgun sequence.
ACCESSION   DASUGB010001088 DASUGB010000000
VERSION     DASUGB010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955322"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VFV87_20640"
     CDS             complement(<1..1157)
                     /locus_tag="VFV87_20640"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5106245.1"
                     /translation="MPTTYVQLPADTNQALGVGQYAIDFLAGRLGQPDAAVLERVEQF
                     HTDSVACAVAALASGMNAPRILREEALTYANARGATCFGSRVRVAPEKAVVANCSAVR
                     ELDANGTNFGYNPRTGQRRGEFGHNDFYPVVVAAAQIAGWKGRRTLLAMLCLDEIRGR
                     LAEAFGLKDHKIDHVLHGAIASAAVYGAILGATAEQIESAIGMVVAHYVPFRAIRHGH
                     QLSDSKGASAAISAEVAVLSMRRAMHGFAGPADIFRNPQAVFCLFEPPQKGSGLFSSP
                     FDLELAAGGDDFAVMGMHFKLGLYEHQSAGAIAGLFDLLTQHPDLPADPAAIEKIRIT
                     IYEPAFSIIGDPHKRDPRTRQSADHSMVYIVATLLRKAARGRSQGTGDRGQG"
     gene            1206..2210
                     /gene="hemB"
                     /locus_tag="VFV87_20645"
     CDS             1206..2210
                     /gene="hemB"
                     /locus_tag="VFV87_20645"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5106246.1"
                     /translation="MDLGPGFPTTRLRRLRYHPAIRSLVCETRLSPASLILPLFARPG
                     KGVRQPIGSMPGHAQLSVDLIAEEAREAYKLGLGGIILFGIPAEKDAAGRDSYSANGI
                     VQQAIKAAKDAAPDLLVITDVCFCEYTDHGHCGLIDDHTGRMDVNNDKTLELLGKQAL
                     SHAQAGADLVAPSGMMDGMVRAIRSALDGGGFEHLPIMSYAAKYASAFYGPFREAAES
                     TPKFGDRRAYQMDPAAGAGQAIREIELDLAEGADVIMVKPALAYLDIVRAARDRFPGV
                     PLAAYNVSGEYSMVKAAAERGWIDEKSVALESLTAITRAGAGIVLTYWAKDVARWLSA
                     "
BASE COUNT          396 a          764 c          698 g          389 t
ORIGIN      
        1 ccctgtcccc tgtcccctgt cccctggctc ctccccctcg ccgccttgcg aagcagcgtc
       61 gccacgatat agaccatcga atgatccgcg ctctgccgcg tccgcgggtc acgtttatgc
      121 ggatcgccga tgatgctgaa agccggctcg tagatcgtga tgcggatctt ctcgatcgcg
      181 gcaggatcag caggcaagtc cggatgctgc gtcagcaggt cgaataagcc ggcaatcgcg
      241 ccggcggatt ggtgttcgta caggccgagt ttgaagtgca ttcccatcac ggcaaaatcg
      301 tcgccgccgg ctgccagctc aaggtcgaac gggctggaaa agaggcctga ccccttttgc
      361 ggcggctcga agaggcagaa gacggcctgc gggttgcgga agatgtcggc cgggccggcg
      421 aagccgtgca tcgctcggcg catcgagagc acggcgactt cggcgctgat cgccgcggaa
      481 gcccctttgc tgtcggaaag ctgatgcccg tggcggatcg cccggaacgg gacataatgc
      541 gccacgacca tgccaatcgc cgattcgatt tgctccgccg ttgcgccgag gatcgccccg
      601 tacacggcag ccgacgcaat cgccccgtgc aggacatggt cgatcttgtg atccttgagg
      661 ccaaaggcct ccgccagccg gccgcgaatt tcatcgaggc agagcatcgc cagcagcgtt
      721 cggcggcctt tccagccggc aatctgggcg gcagcgacga ccaccggata gaagtcgttg
      781 tgcccgaact cgccgcggcg ctgccccgtc cgcggattat agccgaaatt tgtgccgtta
      841 gcgtcaagct cccgcaccgc cgagcaattc gcgacgactg ctttctcggg cgcgacccgt
      901 acgcgactgc caaagcatgt cgcgccgcgt gcattggcat atgtcagcgc ctcctcgcgc
      961 aagatcctcg gagcgttcat cccgctggcc aaggccgcca ccgcacaggc cacgctgtcg
     1021 gtatggaatt gctcgacccg ttccagcacg gccgcatccg gttggccaag ccgaccagcc
     1081 agaaagtcga tcgcgtattg cccaacgccg agggcctggt tcgtatcggc ggggagttgg
     1141 acgtaagtcg ttggcataat tcgctcgccg ttggagcagc gcgggccttg aactataacg
     1201 gagctatgga ccttggaccc ggatttccaa ccacccgcct ccgccgactg cggtatcacc
     1261 ccgcgatccg cagcctcgtg tgcgagacgc ggctctcgcc ggccagcctg attttgcccc
     1321 tatttgcccg gccggggaag ggcgtccgcc agccgatcgg ctcgatgccc ggacatgcgc
     1381 agctttcagt cgatctcatc gccgaggaag cccgcgaagc ttacaagctt ggcctcggcg
     1441 gcatcatctt gttcggaatt ccggccgaaa aagacgccgc cggtcgcgat tcgtactcgg
     1501 ctaacggcat cgtccagcag gcgatcaaag ccgcgaagga cgccgcgccc gacctgctgg
     1561 tcattaccga cgtctgtttc tgcgaataca ccgaccacgg gcactgcggg ctgatcgacg
     1621 atcacacggg caggatggac gtcaataacg acaagacttt ggagttgctc ggcaagcagg
     1681 ctctcagcca cgcgcaggcc ggcgccgacc tggtcgcacc cagcggcatg atggacggca
     1741 tggtacgggc catccgctcg gccctcgacg gcggcggctt cgagcacctg ccgatcatga
     1801 gctacgccgc caaatacgcc agcgcctttt acggcccctt ccgcgaagcg gctgagagca
     1861 cgcccaagtt cggcgaccgc cgggcctatc agatggaccc cgctgccggc gccgggcagg
     1921 cgattcgcga gatcgagctc gacctggccg aaggggccga cgtcatcatg gtcaagccgg
     1981 ccctggcgta cctggatatc gtccgcgccg cccgcgaccg ctttcctggc gtcccgctgg
     2041 ccgcctacaa cgtctccggc gagtattcca tggtcaaagc ggcggccgag cgcggctgga
     2101 tcgacgaaaa gtcggtcgcc ctcgagtccc tcacggccat cacccgcgcc ggcgcgggca
     2161 tcgttctgac gtattgggcc aaagacgtcg cgcggtggct gtccgcgtag gacatctggc
     2221 cgatagtcgg accgcctcca tttgcta
//
LOCUS       DASUGB010001089         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_955352, whole genome shotgun sequence.
ACCESSION   DASUGB010001089 DASUGB010000000
VERSION     DASUGB010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955352"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFV87_20650"
     CDS             complement(<1..1007)
                     /locus_tag="VFV87_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106247.1"
                     /translation="MISRYLITVVLLLLAAGALRAQGEGNDAPRSSPLEGLEKIEPAG
                     SRSEADDDRRAARVHFLQARLDLGRDDKAAALRHYQRAWRWDPQQPELLREIASLAFE
                     LKRGEEAARYAVLAAEHDPQDALLMRRLAAILTKGRDWSRAIRLYELAERMESRRRKP
                     DGKPDLSELTIRLEMGRLYFLTGDFEKSAAAFAVIRDALLDSDSELSADARELLVGKP
                     GETWALWAEAFLSARRFDEAEALFRKSHEARADAATLAFQLARVAAQRGDAAAAIARL
                     DEYFAERSSVAGIEPYDLLAELNAKRLPDKAAARQATIDRLAKLAKDDGDNLALKRAL
                     AG"
     gene            complement(1041..1970)
                     /locus_tag="VFV87_20655"
     CDS             complement(1041..1970)
                     /locus_tag="VFV87_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106248.1"
                     /translation="MSTNRTAAIAKVYKVLKKHYKPIAPQADRGVLEHLLYACLLENA
                     RFDAADEAFAKLKELYFDWNEIRVTTVTELAEGMPEIPDATEAAQRVKKSLQSIFEGS
                     YSFDMEAMKKQNLGKAEKDLEKINGSTPFVRAYVAQNGLSGHNIPVSRSSLEVLYGVG
                     VINDAEADKGQVPGLERAIPKNKGVEFGSLLHQLGADYQASPGSSKLKGILAEIDPEY
                     KDRMSARQSRLEAEAAAQAEAAKEARQKARAEARAAAAEAKLAPKSAGKDSPKVAPKP
                     APQQEGNAKEKSAAAPKTAKAAASKGLAKKKPR"
BASE COUNT          303 a          659 c          655 g          390 t
ORIGIN      
        1 cccgccagcg cccgcttgag ggccagattg tcgccatcgt ctttggccag cttggcgagc
       61 cggtcgatcg tcgcctgacg agccgccgct ttgtccggaa gtcgtttcgc gtttagctcg
      121 gccagcaggt catacggctc aattcctgca accgacgagc gctccgcgaa atactcatcc
      181 aaacgggcga tcgcagccgc agcgtcgccg cgctgggccg caacgcgagc gagctgaaag
      241 gcgagcgtgg cggcatcggc ccgggcttcg tgcgacttgc ggaacaaggc ctcggcttcg
      301 tcgaaccggc gtgcggaaag aaacgcctcg gcccacaggg cccaggtttc gcccggcttg
      361 ccgacgagca gctcgcgggc gtcggcgctc agctccgaat cggagtcgag cagcgcatcg
      421 cgaattaccg cgaacgcggc ggcagatttt tcaaagtcgc cggtcaggaa ataaagccgc
      481 cccatctcca gccggatcgt caactcgctc aggtcgggct ttccatcggg cttgcggcgc
      541 cggctctcca tgcgctcggc cagctcgtac agccggatcg cccgcgacca atcgcggccc
      601 ttggtcagaa tcgccgccag gcgtcgcatc aggagggcgt cttgcggatc gtgctcggcg
      661 gccagcacgg catagcgggc ggcttcttcg ccgcgcttca gctcgaaggc caggctggcg
      721 atctcccgca acagctccgg ctgctgcggg tcccaccgcc aggcccgttg atagtggcgg
      781 agggccgcgg ccttgtcgtc gcgacccagg tccagacgtg cctgcagaaa atgcacgcgg
      841 gcggcccggc gatcgtcgtc cgcttcgctg cggctgcctg cgggctcgat tttctccaga
      901 ccctccagtg gcgaagaacg cggcgcgtca ttcccctcgc cttgcgcgcg cagcgcaccg
      961 gcggccagca ggagcaagac gacagtaatc aggtagcgtg aaatcatgcg gcgcaccgtc
     1021 cggcgggagc cgggcgcagc tcagcgcggc tttttcttgg caagaccttt ggaggccgca
     1081 gctttggccg tcttgggcgc agcggcagac ttttccttgg cgttgccttc ttgttgcggc
     1141 gcgggcttgg gcgctacctt gggcgagtct ttgccggccg atttgggcgc gagcttggct
     1201 tccgctgcgg ctgcacgggc ctcggcgcgg gctttttggc gggcttcctt ggcggcttcc
     1261 gcctgagcgg ccgcttcggc ttcgagccgg gactgccggg cgctcatgcg gtccttatat
     1321 tccggatcga tttcggccag gatgcccttc aacttgctgg aaccaggcga cgcctgataa
     1381 tcggctccga gctgatgcaa cagcgagccg aattcgaccc ccttgttttt gggaatcgcc
     1441 cgctccagcc ccggcacctg gcctttgtcg gcttcggcgt cgttgatcac tcccacgccg
     1501 tacagaacct cgagcgagct tctgctgacg ggaatgttgt gtccgctgag gccgttctga
     1561 gccacatagg cccgcacaaa cggcgtgctg ccattgatct tctccaggtc cttctcggcc
     1621 ttgccgagat tctgcttctt catcgcctcc atgtcgaagg aatagctgcc ctcgaagatc
     1681 gactgcagcg acttcttcac ccgctgcgcg gcctcggtcg cgtcgggaat ctcgggcatg
     1741 ccttcggcca gctcggtcac cgtggtgacg cggatttcgt tccagtcgaa atacagttcc
     1801 ttgagcttgg cgaaggcttc gtcggcagcg tcaaagcgcg cgttctccag caggcaggca
     1861 tagagcaagt gctccaacac gccgcggtcc gcctgcggcg cgatgggctt gtaatgcttc
     1921 ttgaggacct tgtagacctt ggcgatggca gcggtgcggt tggtggacat gcggcagcgg
     1981 gcaggagaca gggttcaggg gacaggc
//
LOCUS       DASUGB010001090         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_398907, whole genome shotgun sequence.
ACCESSION   DASUGB010001090 DASUGB010000000
VERSION     DASUGB010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398907"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..243
                     /gene="rpmG"
                     /locus_tag="VFV87_20660"
     CDS             79..243
                     /gene="rpmG"
                     /locus_tag="VFV87_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5106249.1"
                     /translation="MAKSKKKAEVVFLVCDETGDQNYVLRRKPGGEKLKLKKYSPRVR
                     KHTLHSEKKK"
     gene            326..2101
                     /gene="recJ"
                     /locus_tag="VFV87_20665"
     CDS             326..2101
                     /gene="recJ"
                     /locus_tag="VFV87_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5106250.1"
                     /translation="MTTALHGRTNKRWRFVPHDAERISRLERLAGLPPIVAQLLLSRG
                     VYEPESARTFLDCKLTGLRDPEELPGASAAADRIHAAVRERRKIVVYGDYDADGMTGT
                     AILLSCLKLLGAEVSYYVPNRLEEGYGLNCDALRSLAERGASLVVSVDCGIASIEEAA
                     LAKELGLELIITDHHEFGLQLPEAAAIVHPRLPGTSYPFGGLCGAGVALKLAWAICQR
                     ASQAKRVSEPLRNFLLSAVGLAAIGTVADVVPLLDENRIIVRHGLNSLKYSPPLGLAE
                     LMRVAKLSEKPLLDSEDIGFTIGPRLNAAGRFGQAQLAVELLTTESTDRAQQLAEYIH
                     ELNSSRESLERSIQLAAAKQIKEQFDPDGDAALVLAGRGWHAGVIGLVAGRLAEKYHR
                     PVVLISLDQLGIKPGIGSARSPNGLNLHAALAACSEHLAGFGGHACAAGLRIDERHVD
                     SFRAAFCEHAGSESNGAERIGEIRIDAEAPFCQLTAETVQQIQALSPFGCGNPRPVLC
                     ATGVKLAEPPKRIGANERHLALKLVQQRTGIRGVCFGGGEWHDELAASSAPLDVAYKP
                     VINEFRGRRSVEVQLVDWRISEAAR"
     gene            2135..2500
                     /locus_tag="VFV87_20670"
     CDS             2135..2500
                     /locus_tag="VFV87_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5106251.1"
                     /translation="MDLTIKQGDGYTLVQTHGPLDEGLRDTFREHLHPLVGRSGVNLL
                     IDLSDSPRINSPGVGALVALVADANTHGNRVILLSPQSFVTVVFNVTRLNNYFEIAAN
                     PEEALERLKAGQASSANSG"
BASE COUNT          489 a          850 c          864 g          458 t
ORIGIN      
        1 tcgaccttaa actacgacat tcgtcgaatc gagcattcag cggcaatagc cgctcttctg
       61 gagccggagt tccgcccgat ggccaaaagc aaaaagaaag ctgaagtcgt gtttctcgtc
      121 tgcgacgaga cgggggacca gaattacgtt cttcgccgca agccgggagg cgagaagctg
      181 aagctgaaga agtattcgcc ccgcgtccgc aagcacacgc tgcactcgga gaagaagaag
      241 tgatcgtggg gcgggcgtct cgcctgccat tgaggatcgg gatcgcaatc gcgcgggcag
      301 gcgctggcgc ctaccccacg ggaatatgac caccgccctt cacggacgaa cgaacaagcg
      361 ctggcggttt gtgccgcacg acgcggagcg catctcccgg ctggagcgct tggccggctt
      421 gccgccgatc gtcgcccagc ttcttctcag ccgcggagtt tacgagcctg aatcggctcg
      481 aacctttctc gattgcaagc tgacgggcct gcgcgatccg gaagaactgc ccggcgcaag
      541 cgccgcggcc gatcggattc atgccgcagt ccgcgagcgg cggaagatcg tcgtctatgg
      601 cgattatgac gccgacggca tgaccgggac ggcgattctc ctttcgtgcc tgaaactgct
      661 cggcgccgaa gtcagctatt acgttcccaa tcgcctcgaa gagggctatg ggttgaactg
      721 cgacgccttg cggtcgctgg ccgagcgcgg agcgtcgctg gtcgtctcgg tcgattgcgg
      781 cattgcgagc atcgaggagg cagcactggc gaaagaactc gggctggaac taatcatcac
      841 cgaccaccat gagttcggcc tgcagttgcc ggaagcagcc gcgatcgtcc acccgcgctt
      901 gccgggaacg agttatccgt ttggcggctt atgcggggcg ggtgtggcac tgaagctcgc
      961 ctgggcgatt tgccagcggg ccagccaggc gaagcgcgtc agcgagccct tgcggaactt
     1021 cctcctctcg gctgtcggac tggcggcgat tggaacggtg gccgacgtcg tgccgctgtt
     1081 ggatgagaac cggatcatcg tccggcacgg cctgaacagc ctcaaatatt ctccgccgct
     1141 cggcctggcc gagctcatgc gggtcgccaa gctgagcgaa aagccgctgc tcgacagcga
     1201 agacatcggg tttacgatcg gcccgcgatt gaacgcggcg ggccgcttcg gccaggccca
     1261 gcttgcggtc gagttgctga cgaccgaatc gaccgaccgt gcccagcagc tcgccgagta
     1321 catccacgag ctcaactcca gccgcgagag cctggaacgc agcatccagc ttgccgccgc
     1381 caagcagatc aaggagcagt tcgatcccga cggcgacgcg gccctcgtgc tggccggacg
     1441 cggctggcac gcgggagtga tcggcctggt cgccgggcgg ctggcagaga agtaccaccg
     1501 gccggtcgtg ctgatttcgc tcgaccagct cggtatcaag ccaggaatcg gctcggcccg
     1561 ctcgcccaat ggcctcaatc tgcacgctgc gctggctgct tgctcagagc atctggccgg
     1621 cttcggcggg cacgcctgcg ccgccggatt gcggatcgac gaacggcacg tcgattcgtt
     1681 tcgggctgcc ttctgcgagc acgccggcag cgagtcgaac ggggccgagc ggatcggcga
     1741 aatccgcatc gatgccgaag cgccgttctg ccagctcacc gccgaaacgg tccagcaaat
     1801 tcaggcgttg tcgcctttcg gctgcgggaa tccgcggccg gtgctctgtg cgacaggggt
     1861 caagctggcc gagccgccca agcgcattgg cgccaacgaa cggcatctgg cgctcaagct
     1921 cgtgcagcag cggaccggca tccgcggcgt ctgctttggc ggcggcgaat ggcacgacga
     1981 gctagccgca agcagcgccc cgctggacgt cgcctacaag cccgtcatca acgaattccg
     2041 cgggcggcgg agcgtggaag tgcaacttgt cgattggcgc atctcggaag ctgcacggta
     2101 agtacgaaca cacccggagc gtctggagtt tcccgtggac ctgacgatca agcaaggtga
     2161 cggctacacc ctggtgcaaa cgcatggccc cttggacgaa ggcctgcgcg acaccttccg
     2221 cgagcacctg cacccactgg tgggccgcag cggcgtgaac ctgctgatcg acctgtccga
     2281 ttcgccacgg atcaattccc cgggcgtggg agctctcgtg gctctggtgg ccgatgccaa
     2341 cacgcacggc aatcgcgtca tcctgctcag cccccaatcg ttcgtgaccg tcgttttcaa
     2401 tgtgacccgc ttgaacaact acttcgagat cgccgcaaac cccgaagaag cgctcgagcg
     2461 tttgaaagcc ggtcaggcaa gttcggcaaa ctccggctga gattcgtcat gggatgtagc
     2521 cagggcttga gacgtggcgc tagaatgaaa gtggatcgat cttccggccc gccgcctggt
     2581 cgcgggcatg cggcgccccg gcggctggcg ggcccttggc gaaagcgagg tgccttatgt
     2641 ccatgccacg ctggattccc c
//
LOCUS       DASUGB010001091         4737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_815889, whole genome shotgun sequence.
ACCESSION   DASUGB010001091 DASUGB010000000
VERSION     DASUGB010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815889"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFV87_20675"
     CDS             complement(<1..238)
                     /locus_tag="VFV87_20675"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5106252.1"
                     /translation="MGRALHPGKTRHARCPLNPTQPCRKLTFYSCFISYSTKDQDFAD
                     RLYADLQARGVRVWFAPHDIQGGRKIHEQIDEAIR"
     gene            complement(187..510)
                     /locus_tag="VFV87_20680"
     CDS             complement(187..510)
                     /locus_tag="VFV87_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106253.1"
                     /translation="MLLQPHPLLWTAIENACTIRFTYHGKDRLVEPHDHGILNGSVQL
                     LSWQVAGRSTRRLPNWLLAKVDEMTGLVLLDQTFPGGRPTASGKHIKWDVLFIRVRPA
                     THAAH"
     gene            complement(569..1210)
                     /locus_tag="VFV87_20685"
     CDS             complement(569..1210)
                     /locus_tag="VFV87_20685"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX5106254.1"
                     /translation="MSAKRASPKAKSDHVFGQARSGSKMLRAGLYARVSTNDQQALAM
                     QNRAMREYAARRGWMIAMQVREVNSGAMKREAREKLLEAARRREIDLVLVWRLDRWGR
                     SVTDLLATLQELGHLGVGFVSLTEALDLTTAAGRAMAAMLAVFASFEREVLQERTRAG
                     LAHARANGKRLGRPATAAVHAAEIRKLHRDGISKSEIARRLRIGRTSVRRLLG"
     gene            complement(1266..2801)
                     /locus_tag="VFV87_20690"
     CDS             complement(1266..2801)
                     /locus_tag="VFV87_20690"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX5106255.1"
                     /translation="MKRARRRVDVNLEELDRVLDGAREAPLNEADYDKLKDALHAMAA
                     LLARSANTEKTSAVVGDAAAPEGGEGAGSDSEPPPAGHGRNGAAAFAGANRVAIPHQS
                     LKHGDGCPECGQGNVYAQKEPKALLRIVGQAPLAATVYSLERLRCGACGQVFTAEEPE
                     GVGPDKYDETAAAMIAQLKYGSGIPFYRLERLENQLGIPLPAATQWEMVEELAEVIKP
                     ARDELIRQAAQGEVVHNDDTSMKVLKLEREPGDRRTGVFTSGIVSTAQGRKIALYFTG
                     RQHAGENLADVLKQRAVTAGPVIQMSDALSWNAPKLPEGVNLLVAHCLAHGRRQVVEV
                     AQNFPVECRYVLEQLGEVYGVDAQARDAGMTAEERLRFHQQHSKPILEALHGWLEAQF
                     AERKVEPNSGLGKAITYLLRHWKALTLFLRQAGAPLDNNLCERALKRAVLHRKNALFY
                     RTLNGAQVGDLFMGLIHTCELCGANPFDYLTELQLHAHELATSPSEWMPWNYRETLAR
                     GEI"
     gene            complement(2798..3673)
                     /locus_tag="VFV87_20695"
     CDS             complement(2798..3673)
                     /locus_tag="VFV87_20695"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HEX5106256.1"
                     /translation="MATLRYRGREIHPEDILYIRALIEQHPGDSRRKLSTRLCESWDW
                     RQSNGSLRDMVCRGLLLMLERAGEIALPPVAYKRHNPLAQRARPAPLLLDQTPIEAKL
                     KDLGPIELEQARRTADEPLFNSLMEEHHYLGYEQPVGEHLKYLAWAQGRPIACLAWSS
                     VPRHLGSRDRYIGWSAEARRRNIRFLAYNTRFLILPWVKVEHLASHILGRMASQISDD
                     WQRMYGHPIYFLETFVDPERFRGTCYRAANWVVMGKTTGRGKQSNSYTPNRSIKEVLG
                     YPLTRRFRELLEQSE"
     gene            complement(3719..4078)
                     /gene="tnpB"
                     /locus_tag="VFV87_20700"
     CDS             complement(3719..4078)
                     /gene="tnpB"
                     /locus_tag="VFV87_20700"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX5106257.1"
                     /translation="MIQITAQMRVLVAIEPVDGRKGIDSLARLCQEKLGEDPFSGCVF
                     VFRSRSGTAIRLLTYDGQGYWLAQKRLSKGKFSWWPEAGEPAKPLEAYEAQLLLAAGD
                     LSRVRAAPMWRRVTALK"
BASE COUNT          824 a         1613 c         1367 g          933 t
ORIGIN      
        1 cgcggatggc ctcgtcaatc tgctcgtgga ttttccggcc gccctgaatg tcatgcggcg
       61 cgaaccaaac gcggacgccg cgcgcctgga gatcggcgta gagacggtcg gcgaagtcct
      121 ggtccttggt ggagtacgag atgaaacagg agtagaacgt gagctttcgg caaggttgcg
      181 tagggttcag tgggcagcgt gcgtggcggg tcttacccgg atgaagagca cgtcccattt
      241 gatgtgttta ccggatgcgg tgggccgccc gcccggaaag gtctgatcca gcagcaccag
      301 gccggtcatc tcatcgacct tcgccaagag ccagttggga aggcgacgag tgctgcggcc
      361 ggcaacttgc cagctcagca actggacgga accattgagg attccgtggt cgtgcggttc
      421 gacaaggcga tctttgccgt gataggtaaa ccgaatcgtg cacgcatttt caatagccgt
      481 ccagagcagc ggatggggtt gcagcagcat taattacact ttgacgcggg tccgaaaaat
      541 cgcggggcct atttctcaca gaaataggtc aacctagaag tcggcgaacc gatgtgcggc
      601 cgatccgcaa gcggcgggcg atttcggatt tgctaatgcc gtcgcggtgc agctttcgga
      661 tttcggcagc gtggacagcc gcggtagccg gccgacccag tcgtttcccg ttcgcccggg
      721 catgcgccag acccgcccga gtccgttcct gcaagacctc gcgttcgaag ctggcgaaga
      781 ctgcgagcat cgcagccatc gctcgtccag ccgctgtcgt cagatcgagg gcttcggtca
      841 gcgacacgaa gccgacgccc agatggccca gttcctgtag tgtggccaac agatccgtga
      901 ccgatcgccc ccagcggtcc agtcgccaca ccagcaccag gtcgatctcc cgccgacggg
      961 cggcctccag cagtttctca cgggcctcgc gcttcatcgc accggagttc acctcgcgga
     1021 cttgcatggc gatcatccag ccgcgccggg cggcatactc ccgcatggca cggttctgca
     1081 tcgccagcgc ctgctgatca ttcgtcgaga cacgggcgta caggcccgcc cgcaacattt
     1141 tcgacccgct ccgtgcctgg ccaaaaacgt ggtcggattt tgccttggga gaggcccgtt
     1201 tggcggacat ttcaggatgt tacccagcct tggccgcagt ggatcctttt cggccaggca
     1261 gctgatcata tctcgccgcg cgccagcgtc tcgcggtagt tccagggcat ccattccgat
     1321 gggctggtcg ccagctcgtg cgcgtgcagc tgcaattcgg tcaggtagtc gaacggattt
     1381 gccccacaca gttcgcaggt atggatcagg cccatgaaca gatcgccgac ctgcgcgccg
     1441 ttcaacgttc gatagaacaa cgcattctta cgatgcaaaa cggcccgctt caaggctctt
     1501 tcgcaaagat tattgtccag aggtgcgccc gcctgacgca gaaacagcgt caacgccttc
     1561 cagtgtcgca gcaggtacgt gatcgccttt cccagacccg aattgggctc tactttccgt
     1621 tcggcgaact gcgcctcaag ccagccgtgc agcgcctcca ggattggctt actgtgctgt
     1681 tgatggaagc gcagccgctc ctccgccgtc atgcccgcgt cgcgcgcctg ggcatcgacg
     1741 ccgtacacct cgcccagttg ttccaataca tagcggcatt ccacgggaaa attctgcgcg
     1801 acttccacga cctggcgcct gccatgggca agacagtgcg ctaccagcag gttcacgccc
     1861 tccggcagct tcggcgcatt ccacgacagc gcgtcgctca tctggatgac cgggcctgcg
     1921 gtcaccgccc gctgcttcag cacatcggcc agattctctc cggcgtgctg ccgccccgtg
     1981 aaatacagcg caatcttcct gccctgcgcc gtcgaaacga tgccgctggt gaagacaccg
     2041 gtgcgccggt cgccgggctc gcgctccagc ttcagcacct tcatgctggt gtcgtcgttg
     2101 tgtactacct cgccctgcgc cgcctgccgg atcagctcgt cgcgcgccgg cttgatcacc
     2161 tccgccaact cttccaccat ctcccactgc gtcgctgccg gcagcggtat cccgagctga
     2221 ttttccaatc tctcgagccg gtagaacggg atcccgctgc cgtacttgag ctgcgcgatc
     2281 atcgccgccg cagtctcatc gtacttgtcc ggccccactc cttccggctc ctcggccgtg
     2341 aacacctgcc cgcacgctcc gcaccgcaac cgttccagcg aatacaccgt cgccgccagc
     2401 ggcgcctgcc ccacaatcct cagcagagcc ttcggctcct tctgcgcgta cacattgcct
     2461 tgcccgcatt ccgggcaacc gtcgccatgt ttcaaactct ggtgcgggat cgcaacccgg
     2521 tttgcgccgg cgaacgccgc ggcgccgttc cggccatgcc cggccggagg aggctcgctg
     2581 tccgaccccg cgccctcgcc gccctccggg gccgctgcat cgcccaccac cgcgctggtc
     2641 ttctccgtat tcgccgaccg cgccagcagc gccgccatgg cgtgcagcgc gtccttcagc
     2701 ttgtcgtagt cggcttcgtt caacggcgct tcgcgggcgc cgtccagcac ccggtccagc
     2761 tcctccagat ttacgtcgac gcgtcgcctg gcccgcttca ttccgattgc tccagcaact
     2821 cgcgaaagcg cctggtcagc ggatatccca gaacctcttt aatcgaccga ttcggcgtgt
     2881 agctgttgga ctgcttgccg cgtccagtgg tcttgcccat caccacccag ttcgccgccc
     2941 ggtaacaggt cccgcggaat cgctccgggt ccacgaacgt ctccagaaag tagatcggat
     3001 gcccgtacat ccgctgccag tcgtcgctga tctgcgaggc catgcgaccc agaatgtgcg
     3061 aagccagatg ttccaccttg acccagggca gaatcagaaa tcgagtgttg taggcgagaa
     3121 agcggatatt ccggcggcgc gcctccgcgc tccagccgat gtagcggtcc cgactgccca
     3181 ggtggcgcgg caccgacgac cacgccaggc aggcgatcgg ccgcccctgt gcccacgcca
     3241 aatatttcag atgttcgccc accggctgct cgtaaccaag atagtgatgc tcttccatca
     3301 ggctgttgaa gagcggctcg tcagcggtgc gcctcgcctg ttcgagctcg atcgggccga
     3361 gatccttcag cttcgcttcg atcggcgtct gatccagcag cagcggcgcg ggccgcgctc
     3421 gttgcgccag tggattgtgg cgcttgtaag cgaccggtgg caaggcgatc tcgccggccc
     3481 gctccagcat caacagtagg ccgcggcaca ccatgtcgcg cagggagcca ttgctttgtc
     3541 gccagtccca ggactcgcac aaccgcgtcg aaagcttgcg gcgactgtcg cccgggtgct
     3601 gctcaatcag cgcgcgaatg tacaggatgt cttccgggtg gatctccctg ccgcgatacc
     3661 ggagcgtcgc cattgccccg tagtatgaac ctgtttttct tggaacgcaa ggggaaaatc
     3721 attttagcgc cgtcacgcgc cgccacatcg gcgcggcccg cactcgcgaa agatctcccg
     3781 ccgccagcaa caactgtgcc tcgtaggcct ccagcggttt ggccggctcg cccgcctcgg
     3841 gccaccacga gaatttccct ttcgagagcc gcttctgggc cagccagtac ccctgcccgt
     3901 cgtaagtcaa gagtcggatc gccgtcccgc tgcggctgcg aaacacgaac acgcatccgg
     3961 agaaaggatc ttcgccgagc ttctcctggc acaggcgcgc caacgagtcg atgcccttcc
     4021 ttccgtccac tggctcgatg gctaccagca ctcgcatctg cgcggtgatc tggatcatcg
     4081 ttcggaatcc cgccaggcgc gcagcagatt cgcccagtcc ggcgcaacgg cggccttcca
     4141 atggacgcgc atcttacttc cggcagcgga ttcgaactcg atgacgcatt cgggactgac
     4201 gaccgtcgcc ggcgcgatta gttcaacgaa tctcggctgg cgccgtgttt tcgtcctccg
     4261 cggttcggct gctaccagaa gccgtttcag tttgccggcg tccagacgca attgctgggc
     4321 ggcccgattg acgccttgct gtcgagcggc gctgatagcg agctcccaca actcctccgg
     4381 aatcggcgac tttcgcgaac ggctactcct ccattgctcg aatcgcgtct gcgccacttc
     4441 cattccgccg gccgcttccg tctgttctgc catcgctatc ctcgctcaaa gatgaccata
     4501 gcagtcgcga cctgcaccct tcacgcatgc ttgccgaaag gacaccctgc acctcatgca
     4561 aggtatttgt cgatgctggc tatcggtggg catactggac ccttgaagaa actggagtgg
     4621 tcaaacggca aaataccgtc gaaccagaat cggttcttgc tgccgaggag tattggcgcc
     4681 ctgagccaga cgcccgttcc aagttgctct tagcaggttg cggaaaaagg gttctgg
//
LOCUS       DASUGB010001092         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_676900, whole genome shotgun sequence.
ACCESSION   DASUGB010001092 DASUGB010000000
VERSION     DASUGB010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676900"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFV87_20705"
     CDS             <1..1243
                     /locus_tag="VFV87_20705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5106258.1"
                     /translation="LTRGLYQFYLEEARVALGPEAEDPNKPEALALAAAAESLHAKWP
                     SQEELSGRLQEDGHPILVLWHTTNDGVMALLLGPRWLEGQWTPGFRSSLAADGVAIRL
                     SDMSGQTVIGPQSAPPGHEAAPRLAADTHLPWNLHAISMDPAAALAPVERRRDLVFAG
                     MATIAALILVGAYLIGRTVSRELALSRLQSDFVSSVSHEFRTPLTSICLLSEHLESGR
                     VAGEADRKEYFAVLARESQRLRRLVEGLLNFGRMEAGAVEYRFETVDPAELVQAVSRE
                     FEQDAEARGHRIEVSTKEDTPLVRADRAALACAVWNLIDNAVKYSPECFTVWVDVERI
                     GEFAAIRIRDHGIGIPNSEQTHIFDKFVRGEAAKLSGIRGTGVGLAMVHHIVAAHRGE
                     LQLQSKAGEGSAFSVLLPAVT"
     gene            1247..1921
                     /locus_tag="VFV87_20710"
     CDS             1247..1921
                     /locus_tag="VFV87_20710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5106259.1"
                     /translation="MTRILIAEDDHGIALGLKNDLTIEGYEVEVARDGQAALERARTG
                     AFDVILLDVMMPQKDGYAVCRELRRAGDRTPVLMLTAKVQEAEKVLGLELGADDYVTK
                     PFSSLELRARVRALLRRSAGDSPDVYSFGDVEMDFTRAQVRREGKLVEITPFELKLLT
                     TFVRRRGRILTREQLLDEVWRPDSCPTDRVIDNHIMNLRRKIEPDPQQPRYLVSVRGL
                     GYRFDG"
     gene            2013..>2811
                     /locus_tag="VFV87_20715"
     CDS             2013..>2811
                     /locus_tag="VFV87_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106260.1"
                     /translation="MRRNRHALLTRDFNWLSLKGMKRTIVFVIVAVITAVVAFTGAVA
                     YAQDVSRQANASRGKSNETSIPMVTYSSAATKVSSKKPYTGTLAGTSPFASTLSGSTI
                     PVVVVPVVVNINGTVFDPTAPNFCGPEGGVSAITRIMQSPVVVPSYLTFNGVNVGYTQ
                     YVNGFMRAEFWQTINGNPAYTNEFSFSVAPSVTIQAGSNGITTGSGCQLLGIVNQSFV
                     YNQILALLQSLPGVSPSEIVLFVTNNVARSTASPPTPPATALGYHAVT"
BASE COUNT          543 a          826 c          892 g          550 t
ORIGIN      
        1 cctcacacgc ggactgtatc aattctatct cgaggaagcc cgggttgcgc tggggcccga
       61 ggctgaagat ccgaacaagc cggaagccct cgcactcgcc gctgccgcgg aatcacttca
      121 tgccaaatgg ccgagccagg aagaactgtc cggcaggctg caggaagatg gtcacccaat
      181 actggttctt tggcatacca ctaacgatgg cgtgatggcg ttgttgctgg ggccgcgttg
      241 gctcgaggga cagtggacgc ccggcttccg gtcctccctg gccgctgatg gcgtcgcgat
      301 tcgtctcagc gacatgtccg gccaaaccgt catcggcccg caaagcgcac cgccaggaca
      361 cgaggccgcc ccgcgtctgg ctgccgacac gcacctgccg tggaacctgc atgccatcag
      421 catggatccg gccgcggcgc tggcgccggt cgagcggcgc cgggatctcg ttttcgccgg
      481 tatggcgaca atcgccgcgt tgatcctcgt gggcgcttac ctgatcggcc gtactgtgtc
      541 gcgcgaactg gcgctcagcc ggctgcaatc ggatttcgtt tcgtcggtct cccatgaatt
      601 ccgcacgccg ctcacctcga tctgcctgct gagtgagcat ctggagtccg gccgcgtggc
      661 gggtgaagcg gaccggaagg aatattttgc cgtgctggca cgggaaagcc agcgactgcg
      721 aagactggtg gagggcctgt tgaacttcgg ccgaatggag gcgggcgcgg tggagtaccg
      781 tttcgagacc gtcgaccccg ccgagttggt gcaagccgtg agccgcgaat tcgaacaaga
      841 tgccgaggcg cgtggccatc gcatcgaggt cagcacaaag gaggatactc cgctggtacg
      901 cgctgatcgc gcggcgttgg cctgcgcggt ctggaacctc atcgataatg ctgtgaagta
      961 ctcgccggag tgcttcacag tgtgggtcga cgtggaacgc atcggtgagt tcgccgccat
     1021 tcgcatccgc gaccacggca tcggcattcc caactccgaa cagacgcaca tcttcgataa
     1081 gttcgtgcgc ggcgaggcgg ctaaattgtc gggcattcgc ggcacgggcg taggcctggc
     1141 aatggtgcac cacatcgtgg cggcacatcg cggcgaactt caactgcaaa gcaaggccgg
     1201 cgaaggcagc gccttcagcg tgttgttgcc ggcggtgact tagcaaatga ccaggatcct
     1261 catcgccgaa gatgatcacg gcattgcttt gggtctgaaa aacgacctca ccatcgaagg
     1321 ttacgaggtc gaggtcgcgc gggacgggca ggcggccctg gaacgcgcgc gcacaggagc
     1381 attcgatgtg attctgctcg atgtcatgat gccgcagaag gacgggtatg ccgtttgtcg
     1441 agagctgcga cgcgcgggag atcgcacgcc cgttctcatg ctgaccgcca aggtgcagga
     1501 ggccgaaaaa gttttgggcc tggaattggg agcggacgac tacgtcacca agccattcag
     1561 ttctctagaa ttgcgcgcac gtgtgagggc gctgttgcgc cgttcggctg gcgatagccc
     1621 ggatgtgtat tcgttcggcg acgtggaaat ggatttcacg cgcgcccagg tacggcgaga
     1681 aggcaagctg gtcgagatca cgccattcga gctcaagctg ctcaccacct tcgtgcgccg
     1741 ccgcggccgg attctcacgc gtgagcaatt gctggatgaa gtgtggcggc ccgacagttg
     1801 cccgacggac cgcgtgatcg acaaccacat catgaacctg cggcggaaga tcgagcccga
     1861 tccgcagcag ccgcgctacc tggtgagcgt acgggggttg ggctaccggt ttgacggatg
     1921 acggatcgga ttgaaagatc tgacggaatt ctgaccgatc cccgaaacgg tcctgatggg
     1981 aggagcgcag tgttgatgag gagggagatt acatgcgacg aaatcggcac gcgctgctta
     2041 ctcgggattt caactggctg tctttgaagg gcatgaagag aacaatcgtt ttcgtgattg
     2101 tggctgtcat caccgcggtg gtggcgttca caggggcagt tgcctatgcg caggatgttt
     2161 cgcgccaggc gaatgcgagc agaggcaaat ccaacgagac cagtattccg atggtgacct
     2221 actcctccgc cgccacgaaa gtcagctcga agaagcccta caccggcacg cttgcgggga
     2281 ccagcccgtt tgcgagcacg ttgtccggca gcacgattcc cgtggtggtt gttccggtgg
     2341 tggttaatat taatggaacc gtcttcgatc caaccgcgcc gaacttttgc ggccccgaag
     2401 ggggcgtctc cgccatcact cgcattatgc aatcgccggt cgttgtgccg agctatctca
     2461 ccttcaacgg cgtcaacgtc ggctacaccc aatatgtcaa cggattcatg cgggccgagt
     2521 tctggcagac tatcaacggc aatccggctt acacgaatga gttcagtttt tcggtggctc
     2581 cgtccgtaac gatccaggcg ggctctaacg gcatcacgac tggcagcgga tgtcaactgc
     2641 ttgggattgt gaaccagtct tttgtgtaca accagatcct ggctctgctg caatccctgc
     2701 cgggcgtctc gccctccgag attgtgttgt tcgtgaccaa taacgtcgcc agatccaccg
     2761 caagcccgcc cacgcctcca gccacggcgc tgggctatca cgcggtcacc g
//
LOCUS       DASUGB010001093         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1094064, whole genome shotgun sequence.
ACCESSION   DASUGB010001093 DASUGB010000000
VERSION     DASUGB010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094064"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..494
                     /locus_tag="VFV87_20720"
     CDS             123..494
                     /locus_tag="VFV87_20720"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5106261.1"
                     /translation="MSEPKYVRVERNGEALIVSPLFTFARFTDDDIADEWSQVQSQID
                     GADVKHVLVDLGQIPYFGSTVLEWMVQMWKRVKAKDGKLATCNASPIGREVLTAARFD
                     ILWGICGSREEALAWLAAGGE"
     gene            complement(888..1805)
                     /locus_tag="VFV87_20725"
     CDS             complement(888..1805)
                     /locus_tag="VFV87_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106262.1"
                     /translation="MMKRVSFLLLLAMSLSVAAPGCGGGSGGGKSLTQQVQDAARISD
                     ASLRAKKLAGLAEKQHATGDILGTASTLGAAREAALSVSDPSSKASTLIVVAASNAKT
                     EQSTDDVKSLLKEAGKAIEGVSDPFAQVPLLADLAAATGTHVKNTALAASHLQTAEEA
                     AAKIENVSIRAASLAKIAIAYGQLEGRSEDANRVADAGLEFAHSQADPRDKCDCLGEI
                     AIALAKLQRSDDAAAAFEEAQAAAGEIATDESKAHALLGLGRKARLAKNQSLAATLFD
                     RALDVAESVPDASVRGPLIDEIQADRNKA"
     gene            complement(1880..3112)
                     /locus_tag="VFV87_20730"
     CDS             complement(1880..3112)
                     /locus_tag="VFV87_20730"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5106263.1"
                     /translation="MSDPYFQSLFAERIGGASYGKGTEIYKFEKIKRAKRKALVDHPN
                     RPLLDFGIGENDAMAPESVRRKMAEEINQPANRGYMDNGNQRFKEAAAAFMRRTFGVQ
                     LDPATQVNHAIGSKPALAMLPACFINPGDVTLLTVPGYPVAGTHTKYYGGSVFRLPLL
                     AENDFFPDFGGIPADVWTKTKLLVLNYPNSPTGKTAPRAFYEKVVALAKQHEFVVVQD
                     AAHSLLSYDQPPSSFLAVPGAMDVGVEVHSLSKGFDMIGWRIGWVCGHERIVRAFADV
                     KDNSDSGQFGAIQNAAAAALDDDEIPKRTRAKYQRRLEKLVATLRRCGFQCRMPGGTY
                     FLYTKAPSGLADGTKLETAEAASQYFITQHSIVTVPWDDAGPFLRFSVTYEAADEAAE
                     DALMSAAEDRLRQCRLVF"
     gene            complement(3582..4121)
                     /gene="pyrE"
                     /locus_tag="VFV87_20735"
     CDS             complement(3582..4121)
                     /gene="pyrE"
                     /locus_tag="VFV87_20735"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5106264.1"
                     /translation="MYDKAALMDLVRKLALQLGDFTLASGKKSKYYLDCRKVTLDSAG
                     ANLIADGMLELMGDKLPDAVGGMAIGADPITAAIITVAGRGGKPLRGFIVRKEAKQHG
                     TGRDVEGPVQPGDRVIVVEDVVTTGGSSLAAIEKAEAFGLQVLGVLAIIDRLEGGRET
                     LAARGYSLQTLLTIRDFGL"
     gene            complement(4206..>4488)
                     /locus_tag="VFV87_20740"
     CDS             complement(4206..>4488)
                     /locus_tag="VFV87_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106265.1"
                     /translation="GQFERDLDRLLERVAVPGRTIVMFELPLPPLANEYGRIQRRLAA
                     KYGVRLIPKRVFISILAADGTTLDSIHLSDAGHQRMADAVWSIIEPAYR"
BASE COUNT          773 a         1421 c         1404 g          890 t
ORIGIN      
        1 gtcagcggcc gcgggctgct cctgatgcgg acattcatgg acgacgtcca gttcaatgaa
       61 acgggcaatc aggtgacgat gctcaagcgg tgcggggcgg aaactttgac ggaggtcgcc
      121 tcgtgagcga gcccaaatac gtccgtgtcg aacgaaatgg cgaggcgctt atcgtttcgc
      181 ccctgttcac atttgcgcgg ttcacggacg acgacatcgc cgacgaatgg agtcaggtcc
      241 aatcgcaaat cgacggagcc gacgtcaagc atgtcctcgt cgatctgggc cagattccgt
      301 attttggctc caccgtgctg gagtggatgg tccaaatgtg gaagcgggtc aaagcgaagg
      361 atggcaagct ggcgacgtgc aatgcctcgc ctatcggtcg cgaggtgctc accgcggcgc
      421 gcttcgatat cttgtgggga atctgtggct cgcgggaaga agcgctggcg tggctggccg
      481 ctggcggcga ataaaccgcg cgcatctgca aaacaaaccg ggaccgggtc cgcgttggcg
      541 gcccggcccc ggttatgtaa ataaccttcg gctgatggcg acttagcgga gtcgctggga
      601 gagtggcggg gtctggcgtg ctactgcaca agccgtttgg cgacccgggc catcccggaa
      661 ttattggtcc ctcagtcggt atgcagccga aggttgtttc gctgtatgag ttctgctgcg
      721 agtgcttcgc aggcagttac tatatcggac attctagcat acaggttgat tcgaaccgtg
      781 gcaggcgaat caacatcgga agtctaggcg cgggaattcc ggcgggctag ccgtccagcg
      841 gcgaattccg ggcaattctg caagcatttc gcccggcaca cgaaatgcta cgccttgttc
      901 cggtcggctt ggatctcgtc aatcaggggg ccgcgcacac tagcgtctgg cacgctttcg
      961 gccacgtcca aggcgcggtc gaatagtgtt gccgccaagg actgattctt cgcgagacgc
     1021 gctttgcgcc cgaggccaag caaggcgtga gccttgcttt cgtcggttgc aatttcgccg
     1081 gccgcggctt gcgcttcttc gaacgccgct gcggcgtcgt cactgcgctg cagtttggcg
     1141 agggcgatgg cgatctctcc caggcaatcg cacttgtcgc gcgggtcggc ctggctgtgg
     1201 gcgaactcca ggccggcatc cgccacgcgg ttggcatcct cgctgcggcc ttccaactgg
     1261 ccgtaggcga tcgcgatctt ggccagtgag gcggcgcgga tcgagacgtt ttcgatcttc
     1321 gccgcggcct cttcggctgt ctgcagatgg ctcgccgcga gcgccgtatt cttcacgtgc
     1381 gtgccggtcg ctgccgccag atcggccagc agcggaacct gggcgaacgg atcgctgacc
     1441 ccttcgatcg ctttgccggc ttccttgagc agcgacttga catcatcggt gctttgctcg
     1501 gttttcgcat tggacgcggc aaccacaatc aacgtgctcg ccttgctgga cggatcggag
     1561 acggagagtg ccgcctcgcg tgccgcgccc agcgtgctcg ccgtgcccag gatatcgccc
     1621 gtcgcgtgct gcttctcggc gagaccggcg agcttcttgg cccgcaggct ggcgtcgctg
     1681 attcgggccg cgtcctgcac ctgctgcgtg agcgacttgc cgccgcccga gccgccgcca
     1741 cagcccggtg cggcaaccga cagcgacatg gccagcaaga gcaggaacga aactcgcttc
     1801 atcatcgaat tctctatggg tagtgtgcag gcgagtggga cgcgaatcgc ttctcgcgga
     1861 gcgagaagcc tacggtggtt caaaacacca gccggcattg ccgcagccgg tcttcggcag
     1921 cggacatgag cgcgtcctcc gctgcttcgt ctgccgcttc atacgtgacg ctgaatcgca
     1981 aaaacggccc ggcatcgtcc cacggaacgg tgacgatcga atgctgcgta atgaaatact
     2041 ggctggccgc ctcggctgtt tccaacttcg tcccgtccgc caggccgctc ggggccttcg
     2101 tatagaggaa atacgtcccg cccggcatcc ggcactgaaa gccgcagcgt cggagggtcg
     2161 ccacgagctt ttctaatcgc cgctggtact tggcccgcgt gcgcttggga atttcgtcgt
     2221 cgtcgagcgc cgccgccgcc gcgttttgga tcgcgccgaa ctggccgctg tcgctgttgt
     2281 ctttcacatc ggcgaatgcc cgcacaatcc gctcgtgccc gcagacccag ccgatccgcc
     2341 agccgatcat gtcgaagccc ttgctgagcg aatggacctc gacgccgacg tccatcgcgc
     2401 caggcacggc cagaaagctg ctggggggct ggtcgtagct gagcaggctg tgggccgcat
     2461 cctggacgac gacaaactcg tgctgcttcg ccagggccac gaccttctcg taaaaggctc
     2521 gcggcgccgt cttgccggtc gggctgttgg gatagttcag caccaagagc ttcgttttcg
     2581 tccagacatc cgccggaatg ccgccaaaat ccgggaagaa atcgttctcc gccaacagcg
     2641 gcaggcgaaa cacgctgccg ccgtaatact tcgtgtgcgt ccccgcgacc ggatagcccg
     2701 gcaccgtcaa cagcgtcaca tctccggggt tgatgaagca cgccggcagc atcgccaggg
     2761 ccggcttgct gccgatggcg tggttgacct gcgtggccgg atcgagctgg acgccgaacg
     2821 tccgccgcat gaaggccgca gccgcctcct tgaaccgctg gtttccgttg tccatgtacc
     2881 cgcggtttgc gggctggttg atctcctcgg ccatcttccg ccggaccgat tccggggcca
     2941 tcgcgtcgtt ctcgccgatg ccaaagtcga gcagcgggcg attcggatgg tccacgagcg
     3001 ccttccgctt ggcccgcttg atcttctcga acttgtagat ttcggtcccc ttgccgtaac
     3061 tggcgccgcc aatccgctcg gcaaacaggg actgaaaata gggatcgctc atgggttttg
     3121 tgtggcggaa atcagctgat ttggtgttct cggcagctta caggagcctg ctagggtgga
     3181 caaccgccgc gggggcgtgc atatcagcca tgcgactcat gaccaatacc cctcttcgcg
     3241 caacattcac cccccgatgt tgcgcgtgtg gcgaactttt ttcgcgcagt acaccgtttt
     3301 aactccggcc aataatcctt gctgcataac gatttgcgtc aacgcatccc cccataagag
     3361 gatgttgcgc tgttgcgcgt ttttgtaaac gcgctaggtg gccgcgcttg cccaacatcc
     3421 ctttctcaac cgtgcgttct ccgcccccag ccggtcgcac gtccgatagc ttactaaaaa
     3481 cgcacttgtg aacaaattgt acttaactgg cttgaaagcg attcgagcga acttcccagc
     3541 cgatttcgcc cgacagcaca acagcggtcg cggacgaggg ctcacagccc gaaatcccgg
     3601 atcgtcagca gcgtttgcag gctgtagccg cgggcggcca gcgtttcgcg gcccccttcc
     3661 aggcggtcga tgatcgccaa aacgccgagg acctgcaggc cgaaggcctc ggctttttcg
     3721 atcgcggcca gcgagctgcc gccggtggtg acgacgtcct cgacgacgat cacgcggtcg
     3781 cccggctgga cggggccttc cacgtcgcgg cccgtgccgt gctgcttggc ttccttgcgg
     3841 acgatgaacc cgcggagcgg tttgccgccg cggccggcga cggtgatgat cgccgcggtg
     3901 atcggatcgg ccccgatcgc catcccgccg accgcgtcag gcagcttgtc ccccatcagc
     3961 tccagcatcc catcggcgat caggttcgcc ccggccgaat cgagcgtcac cttgcggcag
     4021 tcgaggtagt acttcgactt cttgcccgaa gcgagcgtga aatcgcccag ttgcaaggcc
     4081 agcttgcgaa ccaggtccat cagggcagct ttgtcataca tcggcggctc cgagtgcgag
     4141 gaatcgccga gttgtaccgc gcgccggccg ggtagcggaa ggcgtgagtc ttccggcttt
     4201 ggacttcagc ggtaggccgg ctcgatgatg ctccaaacgg cgtccgccat ccgctggtgg
     4261 ccggcgtcag agagatgaat ggaatcgagc gtcgtgccgt cggcggccag gatgctgatg
     4321 aagacgcgct tgggaatcaa tcgcacgcca tatttcgctg ccagccggcg ctggattcgg
     4381 ccatactcat tcgccagcgg cggcagcggc agttcgaaca tcacgatggt ccttcccggg
     4441 acggcgacac gctccagcaa ccgatcgaga tcgcgctcga attgaccg
//
LOCUS       DASUGB010001094         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1232313, whole genome shotgun sequence.
ACCESSION   DASUGB010001094 DASUGB010000000
VERSION     DASUGB010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232313"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFV87_20745"
     CDS             <1..349
                     /locus_tag="VFV87_20745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5106266.1"
                     /translation="TLDLGRTRPMPTYDYACDACDHTFEQFESITAEPQKKCPKCKKN
                     KLRRLFGAGAGVVFKGSGFYQTDYRSDSYKKAASADKPDAASSSSSSETKSESKPAAP
                     AKSESKPAKSGDK"
     gene            427..618
                     /gene="yacG"
                     /locus_tag="VFV87_20750"
     CDS             427..618
                     /gene="yacG"
                     /locus_tag="VFV87_20750"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX5106267.1"
                     /translation="MPTIRCPICEKHFDPAASPAMPFCSDRCRQIDLGRWLREVYSVP
                     VQRIPDEGEEPSPESPTDN"
     gene            706..1977
                     /locus_tag="VFV87_20755"
     CDS             706..1977
                     /locus_tag="VFV87_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106268.1"
                     /translation="MNEQGVIRRISWRDLFPWLILLRTFRIAISPTLLALAILASIVS
                     FLGWRLAGLAFNPEAVPGAANSTLYDHLPPAARQWLPEAPTAVLEGYFSLAEPLARLF
                     RVELTLRQTAYYAFGTLLTLAIWAFVGGVITRRAVVELGTEMPPGIQSSVTYAGRRYL
                     WYFLTPLYPLLGIVLLAIPIALVGLPLRAGSVENLRWLHDLGVVLAGLVWILVAIAGL
                     AALWLFGGLIFGWPLMWPAISAEKDGDPFEAFSRSFSYVYGKPLHYAFYVLVAATFGA
                     LCWAVVDGATQLVEHFGFWALAWGGGGEHVLRIRELASAVADGRSRDLGDREYLLSFG
                     TLLMGLVVRLMRSVTAAFAHTYFWCAASAIYLLLRMDVDHQEIDEVYLEDDPEKAPAP
                     LPTETAAPAPLSSPPPAPAADSASSDQSDQA"
     gene            complement(1968..2345)
                     /locus_tag="VFV87_20760"
     CDS             complement(1968..2345)
                     /locus_tag="VFV87_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106269.1"
                     /translation="MSAVIFLSSDMLFSSRVLGAGQALGVKCQVVSTSGQIAAAVDEN
                     CRLVLIDLGMASLDLTAAVAAIRASAPTAKIVAFGPHVDEALLESGRAAGCDLVLPRS
                     QFHKQYVDLLRVAAGPGDSPRQA"
     gene            complement(2397..3539)
                     /locus_tag="VFV87_20765"
     CDS             complement(2397..3539)
                     /locus_tag="VFV87_20765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106270.1"
                     /translation="MLTTTRQDVNRTITVGGSLAASRPNELVPLYEQILEEQRLSWTS
                     NLRLLKLLGAGGQGVVYLTELRGTDEFTLPAAIKIFSPERFDDVCNYDEAMCRVARAS
                     ARVAQIQHDNLLYVYNFVDRHRIRMMVMEWIDGYDLARLLVPEMLERVKSRVSQRRWE
                     YVNQVIVTGGPHQPRMKAGVAVAVVRDCLNALAALHRHGIVHGDIKPSNIMLKRTGIA
                     KIVDIGSAFELRDPPQVRCCTLAYAAPEVLENRELIPRSDLCSLGYVLLELLAGQQPF
                     VGISDHRELLEAKRTLPMRLHEILPEEVLRCELLCAFIRGMIAADPARRFPSAEAAEL
                     LKEGAAAFHRMLVMGNLASEYDNDIRLWLEELKELDAQDGVASEAA"
     gene            complement(3697..4032)
                     /locus_tag="VFV87_20770"
     CDS             complement(3697..4032)
                     /locus_tag="VFV87_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106271.1"
                     /translation="MNCQELADRIQRLQPEADTRDVARLCLLLANTTDDVASLSDEDI
                     LTEAWREAGLKLQLATDQHAAMTEELESLARSDPRRFSPEQIWVLIRAIKVQSQILQL
                     YVGQPVLDA"
     gene            complement(4250..>5616)
                     /locus_tag="VFV87_20775"
     CDS             complement(4250..>5616)
                     /locus_tag="VFV87_20775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5106272.1"
                     /translation="LAAASDKDREHLQVLNELGLRSYICVPLLSHGAVLGVLTFATAE
                     SGWHYRQEDLQAAEDLARRVVIAIENANLYRALQEADRRKDEFLATLAHELRNPLAPV
                     RHWLEILKRGGDNSSELAHLARETMDRQVSHMERLIDDLMDISRITRNRLELRCHRVE
                     LATIIGNAVEVSRPLTESFGHTLAVNLPEEPIYLHADPVRLSQVLGNLLSNACKYTPA
                     GGRICLTVERRGQDARIAVSDNGMGISADMLPRIFEMFTQANRASDRAPAGLGIGLTL
                     VKQLVEMHGGSVEAQSAGEGQGSTFVVTLPLLAGHLEVEPPREPAPAAGMDGAATRRV
                     LVVDDNQDGANSLAVLLRLSGHEVQVAHDGLQAVETAGDYRPEIIFLDIGLPKLNGYD
                     VCRQIRGKPWGKEIVIVALTGWGQDGDRRKSQEAGFDQHLIKPVHPDALMRVLDDLPT
                     AVGG"
BASE COUNT          974 a         1852 c         1748 g         1042 t
ORIGIN      
        1 gactttggac cttggacgaa cccgccccat gcccacctac gattacgcct gcgacgcctg
       61 cgaccacacg ttcgagcagt tcgaatcgat caccgccgag ccgcagaaga agtgccccaa
      121 gtgcaagaag aacaagctcc gccgcctgtt cggcgcgggg gccggcgtcg tcttcaaggg
      181 ctcgggcttt taccagaccg attaccgcag cgattcgtac aaaaaggctg cctccgccga
      241 caagccggat gccgcttcgt cgtcgtccag cagcgaaacc aagagcgaaa gcaaacccgc
      301 cgcgccagcc aagagcgaat ccaagccggc caaatcgggc gacaagtaac cgcgggcgac
      361 cgtaggatgg acctcctggt ccgtccatgc cgtttggcgg acggaccagg aggtccaacc
      421 tacgagatgc ccacgatccg ctgccctatc tgcgagaagc atttcgatcc ggcggccagc
      481 ccggcgatgc ccttctgcag cgaccgctgc cggcaaatcg atctgggccg ctggctgcgg
      541 gaagtctatt ccgtgcccgt acagcgcatc ccggatgagg gcgaagaacc gtcgccggaa
      601 agcccaactg acaactgaca actgaccaaa gaccagccta gcccccgcta cccgcgcacg
      661 tcataatcgc ggtcgcacca cgattcgccg ctgggaggcc gcacgatgaa cgagcagggc
      721 gtaatccgcc gcatctcctg gcgcgacctc tttccgtggc tgatcctgct gcgtacgttc
      781 cgtatcgcca tctcgccgac gctgctcgcc ctggcgattc tcgcgtcgat tgtgtcgttc
      841 ctgggctggc ggctggcggg gctggcattc aacccagaag cggtgccggg ggctgccaat
      901 tcgacgctgt acgatcacct tccgccggcc gcccgccagt ggctgccaga ggctccgacc
      961 gcggtcctcg aagggtactt cagtctggcg gaaccgctgg cgcggctctt tcgcgtggag
     1021 ctgacgctgc ggcagacagc ctactacgcc ttcggcacgc tcctgacgct ggcgatctgg
     1081 gccttcgtgg gcggcgtgat cacgcgacgg gcggtcgtgg aactgggaac cgaaatgccg
     1141 ccgggcatcc agtcgtcggt gacgtatgcc ggccggcgtt acctgtggta tttcctgacg
     1201 ccgctctatc cgctcttggg gattgtgcta ctggcgattc ccattgcgct cgttggcttg
     1261 cccctgcggg ccggatcggt cgagaacctc cgctggctgc acgatctggg cgtcgtgctg
     1321 gcgggcctcg tctggatact cgtcgcgatt gccggcctgg cggcgctctg gctcttcgga
     1381 gggctgatct tcggctggcc gctgatgtgg ccggcgatca gcgccgagaa ggacggcgac
     1441 cccttcgaag ccttcagccg ctcgttttcg tacgtctatg gcaagccgct gcactacgcg
     1501 ttctacgtgc tcgtggcggc gacgttcggc gcgttgtgct gggccgtcgt ggacggggca
     1561 acgcaactcg tcgagcactt cggcttctgg gcgctagctt ggggcggagg gggcgagcat
     1621 gtgctgcgca ttcgcgagct ggccagcgcc gtcgccgatg gccgcagccg cgacctgggc
     1681 gaccgggaat acctgctttc ctttggcacg ctgctcatgg gcctggttgt gcggctgatg
     1741 cgctcggtta ccgcggcgtt tgcccacacg tacttctggt gtgccgcgtc ggcaatttac
     1801 ctgctcttgc ggatggacgt cgatcatcag gagatagacg aggtttatct ggaggacgac
     1861 cccgagaagg cgccggcgcc gctgccgacc gagaccgcgg cgcccgcgcc tctgtcgtct
     1921 ccaccgccag cgccggccgc cgattcagcg agcagcgatc agagcgatca agcctgacgc
     1981 ggtgaatcgc caggccccgc ggcgacacgc aacaggtcca catactgctt gtgaaactgc
     2041 gatcgcggca aaacgagatc gcacccggcg gctctaccgg attcaagcaa ggcctcgtcc
     2101 acgtgcggac cgaaggcgac gatcttcgcg gtcggtgcgg aggcccgtat cgccgctacg
     2161 gcagcagtca aatccaaact ggccatgccc agatcgatca gcaccagccg gcaattctcg
     2221 tcaaccgcgg cggcaatctg ccctgacgtg gaaacgactt ggcactttac gccgagcgcc
     2281 tgccccgcgc cgagcacgcg gctggagaag agcatgtcgg aagagaggaa gatgactgcg
     2341 gacatgctca gattggtcgt cgggcagttg cggcaggcaa gaatgcctgc cccacgttag
     2401 gccgcttcgc tggcgactcc gtcctgggcg tcgagctcct tcagctcctc cagccacagg
     2461 cggatgtcgt tgtcgtattc gctcgccagg ttgcccatca cgagcattcg gtgaaacgcg
     2521 gcggcgcctt ctttgagcag ctcggccgct tcggcgctgg gaaaccgccg cgcgggatcg
     2581 gcggcgatca tcccgcgaat gaacgcgcag agcagctcgc accgcagcac ctcttcgggc
     2641 aggatttcgt gcagacgcat cggcagcgtg cgcttcgcct cgagcagttc ccggtggtcg
     2701 ctgatgccga cgaacggctg ctggccggcc aagagctcca agagcacgta gccgagcgag
     2761 cagaggtcgc tgcgcggaat gagctcgcgg ttttcgagca cttccggcgc cgcataggcg
     2821 agcgtgcagc accgcacctg gggcggatcg cgcagctcga acgccgagcc gatatcgacg
     2881 atcttcgcga tgccggtccg cttgagcatg atgttcgacg gcttgatgtc gccgtggaca
     2941 atgccgtgcc ggtgcagcgc ggccagggca ttgaggcaat cgcggaccac ggccaccgcc
     3001 acgcccgcct tcatccgcgg ctggtgcggc ccgccggtca cgattacctg gttgacgtac
     3061 tcccatcgcc gctggctaac gcggctcttg acgcgctcca gcatttccgg caccagcagg
     3121 cgagccaggt cgtagccgtc gatccattcc atcaccatca tgcggatgcg atggcgatcg
     3181 acgaagttat agacgtagag caggttgtcg tgctggatct gggcgacgcg ggccgaagcc
     3241 cgggcgacgc ggcacatggc ctcgtcgtag ttgcagacat cgtcgaaccg ttcgggcgag
     3301 aagatcttga tcgccgccgg cagcgtgaat tcgtctgtgc cgcgcagctc ggtgagatag
     3361 acaacgcctt gtccgccggc gccgagcagc ttcaacagcc gcaggttgct ggtccagctc
     3421 agccgctgct cttcaagaat ttgctcgtac aggggaacca gctcgttggg ccggctggca
     3481 gccaggctgc cgccgacggt gatcgtccga ttcacatcct gcctggtcgt ggtgagcatc
     3541 gcctgcgctc cgcgctgcga ggaaggtgct ccaaatcagc cgaactcatt gtggcccggc
     3601 cgcgccgaat cgtaaagacg agttggctgg agggtgggct ttgcccacca gcgcacgcca
     3661 aggaccgcac cttcggtgtg gtgggcagag cccaccctat gcatccagca ccggctgacc
     3721 gacgtaaagc tgcaggattt ggctttgcac cttgatcgcc cggatgagca cccagatttg
     3781 ttcgggcgag aaccgccgcg gatcgctgcg ggccagcgac tccaactctt ccgtcatggc
     3841 cgcatgctga tcggtcgcta gctgcagttt cagccccgct tcgcgccacg cctcggtgag
     3901 aatgtcctcg tcgctcagcg acgccacgtc atcggtcgta ttggccagca gcaagcacag
     3961 ccgggccacg tcgcgcgtat ccgcttcggg ctgcaagcgc tgaatgcggt cggctagttc
     4021 ttggcagttc atgtctggct ttcactcgat cccgtcgttt cccgaccgcg cccatccgcg
     4081 aatcgctgat ccgacttcta acggaacttt agctctgaat tgcaaatggg gcgagaattt
     4141 ggcatcttcc gcgccataaa ctgcgtcgtt gcacgaagtc cataaagtcg ctgcatattc
     4201 gctcaaaaca atcgagcgag aaatgccgaa tgcgcgggcg ccgcaccgct tagccgccca
     4261 ccgccgtcgg caggtcgtcc agaacgcgca tcagcgcgtc ggggtggact ggcttgatca
     4321 ggtgctggtc gaagcccgcc tcctgcgact tgcggcgatc gccatcctgt ccccatcccg
     4381 tcagtgccac aatcacgatt tccttccccc acggcttgcc gcgaatctgg cggcacacgt
     4441 cgtagccatt gagcttgggc aagccgatat cgaggaagat gatctcgggc cgatagtcac
     4501 ctgcggtttc cacggcctgc agcccgtcat gcgccacttg cacttcatgg ccggaaagcc
     4561 gcaggagcac ggcgagtgaa ttggctccgt cctggttatc atcgacgacg agcactcgtc
     4621 gcgttgccgc accgtccatg cctgcagctg gtgcaggctc gcgcggtggc tcgacttcga
     4681 ggtggccggc gagtagcggc agggtcacga caaacgtact tccctggccc tcgccggcgc
     4741 tctgcgcctc gacgctcccg ccgtgcattt cgacgagctg tttgaccagt gtcagtccga
     4801 ttccgagtcc cgccggcgcg cgatccgaag cgcggttggc ttgcgtgaac atctcaaaga
     4861 ttcggggcag catgtcggcg gagattccca tcccgttatc gctcaccgcg atccttgcgt
     4921 cctgcccgcg ccgctcaaca gtcaggcaaa tgcggccgcc cgcaggcgtg tatttgcaag
     4981 cattgctcag cagattgcct agcacctggc tgagccgcac tggatcggcg tggagataga
     5041 tcggctcttc cggcagattc acggccagcg tgtgcccaaa gctctcggtc agtggacggc
     5101 tgacttcgac ggcatttccg ataatggtcg ccaattctac ccgatggcac cgcaattcca
     5161 gacgattgcg cgtaatgcgt gaaatatcca tcaggtcgtc gatcagcctc tccatgtgcg
     5221 acacttgccg gtccatcgtc tcgcgcgcca agtgcgccag ttccgaactg ttgtcaccgc
     5281 cgcgcttcag aatctcaagc cagtggcgga ctggcgccag tggattgcgc agctcgtgcg
     5341 cgagggtcgc caggaattcg tccttgcggc gatcagcttc ctgcaaagcg cggtacaggt
     5401 tggcattctc aatcgcaatc acgacccgcc gggcgagatc ctccgcagcc tgcaagtcct
     5461 cctgccgata gtgccagccg gactcagccg tcgcgaacgt gagcacaccg agcacggcgc
     5521 cgtgcgaaag aagcggcacg caaatgtatg acctcagccc caattcgttc agcacctgca
     5581 ggtgctcgcg atccttgtcc gatgcggccg caagat
//
LOCUS       DASUGB010001095         6387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_399311, whole genome shotgun sequence.
ACCESSION   DASUGB010001095 DASUGB010000000
VERSION     DASUGB010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399311"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFV87_20780"
     CDS             <1..361
                     /locus_tag="VFV87_20780"
                     /inference="COORDINATES: protein motif:HMM:NF045154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106273.1"
                     /translation="HVDLHVSPAANELEVSDLPRATRYARYQAEQGSAATNLWHESVR
                     LNDLERHLLRRLDGRTDHAELTQSLTSEVEQGQLIVQDGGRALTDLGRVEKIISGAMM
                     ESLGLLARKGFLLKAQS"
     gene            complement(392..1270)
                     /locus_tag="VFV87_20785"
     CDS             complement(392..1270)
                     /locus_tag="VFV87_20785"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX5106274.1"
                     /translation="MIAAVRRDFSSGGVAQVLGGGARTVVVASWLMVAVCWLSSFTPQ
                     DFGFFRSRWSCAVLALLLWQREGIRLSPETVRRGLHRMEFGWRRPRPVVGPRDPQYAT
                     KLRRIRHLIAALPPDETVVFQDEVDVHLNPKIGSCSMRRGQQAEVVTPSNNEKRHLAG
                     SFHWRTGRLLLSALGKRRNAELFVAHLDDVHCRLRSCRVIHVVCDNARFHDCRAVREY
                     LQRHGERIQLHFLPKYAPETNPIERVWWHLHEDLARNHRCTSLDELLQQVYDWTTAGS
                     FYFQTASFRELYALAA"
     gene            1494..2264
                     /locus_tag="VFV87_20790"
     CDS             1494..2264
                     /locus_tag="VFV87_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106275.1"
                     /translation="MVPKSILPPTWDVPREFRERLGEKVGRQRTMLADGHLLLVLHRP
                     PKANDPERTGRLFWRKADGSWQSNDVGGGPAALSRHLHEFADLIDRYDRIEDEAVSVA
                     DYFAVLEALSPLHRTIRNLHAALQDAREKLPDDRDLINARDRAYELERAAELLLADVR
                     NSLEFAVAKKSEEQAAASHQMAAASHRLNMLVALFFPIATLSALFGANLTHPLEQLIP
                     PPYAFLAMIAAGLILGGGLAGYLLTTTQTPAKSPSDRK"
     gene            complement(2268..2456)
                     /locus_tag="VFV87_20795"
     CDS             complement(2268..2456)
                     /locus_tag="VFV87_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106276.1"
                     /translation="MKDEKRFLRELKRSIKKAGNRKRRRYLKDVTAEADEFSFGRDRT
                     DVMNDKARGMKPRPASGD"
     gene            complement(2581..3978)
                     /locus_tag="VFV87_20800"
     CDS             complement(2581..3978)
                     /locus_tag="VFV87_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106277.1"
                     /translation="MKLSMNLAAMLLGVLSFVGLNVARADGVAWPPGLPGAKEGTATL
                     RSQQFLAIPASVAEARSKNGAAEFILAKQPPTIDLAYHGDLGPDAANRRLWSSWGDIC
                     LASDGKVYVGLGDHGDDAGGDARCFLYQWDPQANALKQVVDVNSLLPDIQPHSRWSKL
                     HAKIDEGPDGKIYFSATLNDGNRAGKENYHWSEQTPGGQLYRYDPKTGKAEIFASLPP
                     KRCTATSLVDLERKLWWCNLEAGEGGALFALDLTSGKPVFQAADGSMGFNRNFALAKD
                     GSIYFNGPDGSLWKCDGKTHALVATQSTWGDSPGMRCSTAETKQGEIYGITHKTSQLF
                     RYRPAEDKLELLGPNWLAGSYTAVCVLSPDERFVYYLPGSHGGAFKDGTPVVQYEIAT
                     GRRKVLAFLAAACEKELGYVPAGTYGIKISADGSTLYVNFNGHPADPLRPEKMKPIGF
                     GLTAFAAIQIPDSER"
     gene            4190..5581
                     /locus_tag="VFV87_20805"
     CDS             4190..5581
                     /locus_tag="VFV87_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106278.1"
                     /translation="MALLGSAFASASRGENIDLSTVPSRETVQLTIYNSEDITLVRET
                     RKVTFKKGVNPLQFSWANTLIDPTSVELQFLTQADKLVVLDTTFPHERPQMLYWNVQS
                     DFDGEATIQITYFTSGITWTADYVAIANAEETATKFEGFVRVHNNSGEEYEDAQVRLV
                     VGTINLVEKIANLANISMSEVASLQADQARDLRFKALGMMLPTNGAAPAAQSATPAPK
                     EIIKEGLSEYFIFTIEGTETVPNGWSKRLRSIEAEAVPVKVQYRYRPQEYGDQLVRMY
                     LLTNDEKSQLGTTPLPDGVVRVFRDNGKGGLSFVTQQSIKYIPIGDKIELNLGVDPEV
                     IFELIKLRAFRSDLWLQITGATELRKVGGDVVVKQENASIVGWDDHELYSQRIRNYTG
                     DEIEVEIRRSFDGHVVFRSQLEPKLHDYRTALFLARIAPGKRADLLFEIVRKQGTSAK
                     QNNITLEAAEVKP"
     gene            complement(5736..>6387)
                     /locus_tag="VFV87_20810"
     CDS             complement(5736..>6387)
                     /locus_tag="VFV87_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5106279.1"
                     /translation="LLKAFGAKLYLTPRETGMRGAVGRAVELFQEHGGEPKAFIPQQF
                     KNPANPEVHRKTTAEEIWRATSGQIDILVSGVGTGGTITGCGEVLKARKASVKCIAVE
                     PTASAVLTQHLMQGVPKDQLKAGPHKIQGIGAGFIPDVLNTAVIDDVVQVTDDESFEM
                     GRRLAKEEGMLCGISSGAAVAAAVKVAQRPENAGKVIVVVMPDLGERYLSTQLYPE"
BASE COUNT         1199 a         2068 c         1965 g         1155 t
ORIGIN      
        1 gcacgtcgat ctgcatgtct cacccgccgc caatgagctc gaagtttccg atctgccgcg
       61 cgccacgcgc tatgcccgat atcaggccga gcagggatcg gctgctacga acctgtggca
      121 cgagtcggtt cgcctcaatg acctggagcg gcaccttctg cggcgtttag atggtcgcac
      181 cgatcacgca gagctgacac aatccttgac gagcgaagtg gagcaaggac agctcatcgt
      241 ccaggacggt ggccgcgcac tcaccgatct ggggcgcgtg gagaagatca tcagtggcgc
      301 tatgatggaa tccctgggac ttctggcccg caaagggttt ctgctcaagg cccaaagcta
      361 gactaaatag ctcctcggcc acagcgtagc gctaggcggc gagggcgtat agttcgcgaa
      421 agctggcggt ttggaagtag aatgaaccgg cagtggtcca atcgtaaacc tgctggagca
      481 gttcgtcgag cgatgtgcag cggtggttgc gggcgagatc ttcatggagg tgccaccaga
      541 cgcgttcgat gggattggtt tcgggagcgt atttggggag gaagtggagt tggattcgct
      601 caccatggcg ctgcagatac tcccgcaccg cgcggcagtc gtggaaccgg gcgttgtcgc
      661 agacgacgtg gatcacgcgg cacgagcgga gccggcaatg gacatcatcc aggtgagcca
      721 cgaacagctc ggcgttgcga cgcttgccga gagccgacag cagcaagcgt ccggtgcgcc
      781 agtggaacga cccggccaga tgccgcttct cgttgttgct cggtgtgacg acttcggctt
      841 gctggccgcg gcgcatcgag caggagccga tcttgggatt cagatgcaca tccacctcat
      901 cctgaaaaac gaccgtctcg tccggcggca gagcggcgat caagtgccgg attcgccgca
      961 gtttggtggc gtactgcgga tcccgcgggc cgaccaccgg ccgcggcctg cgccacccga
     1021 actccatccg gtgcaagccc cgccgcacgg tctcggggct gaggcggatt ccttcccgtt
     1081 gccacaacag cagcgccaag acagcacacg accagcggga gcggaagaac ccgaagtcct
     1141 ggggcgtaaa actcgagagc caacaaacgg ccaccatcag ccaggaagcc acgaccaccg
     1201 tccgcgcccc tccgcccagc acctgggcca cgccgccgga ggagaagtcg cgtctcacgg
     1261 ctgcgatcat cgtttctcct taactcgatc gacaatgcca cgctgctcaa actgatcctg
     1321 cgcaatgacg gtcgccgcgt cgaatttcaa cgtccgtgcg caagtcttga atgcgatgca
     1381 acttgcgtct tcacacggac ctcacaaaga gccgccctgc cgcagcgaat caaccggtgg
     1441 gtaacccgcc tgactggttc tgcacgaact gcatttttct cggagggcga tctatggttc
     1501 ccaaatcgat tctcccgccc acctgggatg tgccgcgcga gtttcgcgaa cgtctgggcg
     1561 aaaaagtcgg ccgccagcgg acgatgctgg ccgacgggca cttgctcctg gtcctgcacc
     1621 ggccacccaa agccaacgat cccgagcgca ccggtcggct gttctggcgg aaagccgacg
     1681 gctcgtggca atcgaacgac gtgggcggcg gaccggcggc cctttcgcgg cacctgcacg
     1741 aattcgccga cctgatcgac cgctacgacc gcatcgagga cgaggcggtc agcgtggccg
     1801 attacttcgc cgtgctagag gcgttgtcgc cgcttcatcg cacgatccgc aatctacacg
     1861 ccgctctgca ggacgcccgc gagaagctgc cggacgaccg cgacctgatc aacgcccgcg
     1921 accgggccta cgagctggag cgggcggccg agctgttgct agccgacgtg cggaactcac
     1981 tggaattcgc cgtcgcgaaa aagtccgagg aacaagccgc cgcttcgcac caaatggctg
     2041 ccgcctcgca ccggctgaac atgctggtgg ccctgttctt cccgatcgcc acgctctccg
     2101 ccctattcgg cgccaacctc acacatccgc tggagcaact catcccgccc ccctacgcct
     2161 tcctcgcgat gattgccgcg gggctgattc tgggcggcgg gctcgccggc tacctcctga
     2221 cgaccactca gacgccggca aaaagcccgt ctgaccgcaa gtgactctca gtcgcctgac
     2281 gcagggcgcg gtttcatgcc tctcgccttg tcgttcatga cgtccgtgcg gtcgcggccg
     2341 aacgagaact cgtccgcctc ggccgtgaca tccttcagat accgccgccg cttgcggttc
     2401 cccgccttct tgatcgaccg cttcagctcg cggaggaagc gcttttcgtc tttcattgtg
     2461 ttccgacctg acatgcgaat cgtttagcgc ggagccgaag gcgacgcgcc gcgcgcgtgg
     2521 gctcggcatc gcctgcggcg cgccgctaaa cgacccattt ccgcgactga gggttcgccc
     2581 ctaccgctcc gagtcgggta tctgaatcgc tgcgaaggcg gtgaggccga agccgatggg
     2641 cttcattttc tcggggcgga gggggtcggc ggggtggccg ttgaagttga cgtagagcgt
     2701 gctgccatcg gcgctgatct tgatgccgta cgtgccggcg gggacgtagc ccagctcctt
     2761 ttcgcacgcg gctgccagga aggcgaggac tttccgccgg ccggtggcga tttcgtactg
     2821 cacgaccggc gtgccgtcct tgaaggcccc gccgtgcgac ccgggcaggt aatagacgaa
     2881 ccgctcgtcg ggcgagagga cgcagaccgc ggtgtacgag ccggccagcc agttgggccc
     2941 gagcaattcc agcttgtcct cagccgggcg gtagcggaag agctggctcg tcttgtgtgt
     3001 gatgccgtag atttcgccct gcttcgtttc ggcggtcgag cagcgcatgc cgggcgaatc
     3061 gccccacgtc gattgggtcg ccaccagggc atgcgtcttc ccgtcgcact tccacagcga
     3121 gccgtccggg ccgttgaagt aaatcgagcc gtcttttgcc agggcgaagt tgcggttgaa
     3181 ccccatcgag ccgtccgctg cctgaaagac gggcttgccg ctcgtcaggt cgagggcgaa
     3241 gagcgcgcct ccctcgcccg cttcgaggtt gcaccaccaa agcttccgct ccagatcgac
     3301 cagcgacgtg gccgtgcaac gcttgggcgg caggctggcg aaaatctccg ccttgccggt
     3361 cttgggatcg tagcgataaa gctgcccgcc gggcgtttgc tcgctccagt ggtagttctc
     3421 cttgccggcc cgattgccgt cgttcagcgt tgcgctgaag tagattttcc cgtctgggcc
     3481 ttcgtcgatc ttggcgtgca gcttcgacca gcgcgagtgc ggctggatat ccggcaggag
     3541 cgaattcaca tcgacgactt gcttcagcgc gttcgcctga gggtcccatt gataaaggaa
     3601 gcaccgcgca tcgccgccag cgtcgtcgcc gtgatcgccg aggcccacat agaccttgcc
     3661 atcgctggcc aggcagatat cgccccagct tgaccacagc cggcgattgg ccgcgtccgg
     3721 ccccaggtcg ccgtgatagg ccaagtcgat cgtcggcggc tgcttggcca ggatgaattc
     3781 ggccgcgccg ttctttgacc gcgcctcggc cacactggcg ggaattgcca ggaactgctg
     3841 gctccggagc gtggccgtcc cctccttcgc ccctggcagc cccggcggcc aggccacccc
     3901 atccgcccgg gccacgttta gtccaacgaa cgacaacacg cccagcagca tcgccgccag
     3961 attcatagac aatttcatag gtattcctcc agggccaagg taatgggtgc ccaggagagt
     4021 tgcaaacttg cgggcctcat cagttgccgg aagtcgttat gcggcaataa ctttcccggc
     4081 tttgaactgc ccaaaacgat tcgcccgcgg gcgtcgtctt aatcagcaca ctcatttcgc
     4141 accaggaaca gcaatcatca tgcagcggca tttcctgatc ggcctagcca tggctctcct
     4201 cggaagcgct tttgccagcg cttcgcgcgg cgagaacatc gacctctcaa cggtccctag
     4261 ccgcgagacg gtccagctca caatctacaa cagcgaggac atcacgctgg tccgcgagac
     4321 gcggaaggtg acgttcaaga agggggtcaa tccgctgcag ttcagttggg ccaatacgct
     4381 catcgaccca accagtgtcg aactgcagtt tctcacgcag gccgacaagc tggtggtcct
     4441 cgacaccacc ttcccgcacg agcggccgca gatgctgtac tggaacgtgc agagcgattt
     4501 tgacggcgaa gcgacgatcc agatcacgta tttcaccagc ggcatcacct ggaccgccga
     4561 ttacgtcgcg attgccaatg cagaagagac cgcgacgaag ttcgagggct tcgtccgcgt
     4621 ccacaataac tcgggcgaag aatacgagga cgcgcaggtg cggctggtag tggggacgat
     4681 caacctggtc gaaaagatcg ctaacctcgc caacatttct atgagcgagg tagcaagttt
     4741 gcaggccgat caggcccgcg atctgcgctt caaggccctc ggcatgatgc tgccaaccaa
     4801 cggcgccgcg ccagcggccc aaagcgcgac gcccgcccca aaggaaatca tcaaagaggg
     4861 cctcagcgaa tacttcatct tcaccatcga agggacggaa acggtcccca atggctggtc
     4921 gaagcggctg cggtcgatcg aggcggaggc ggtcccggtg aaggtgcaat atcgctaccg
     4981 gccgcaggaa tatggcgatc agctcgtgcg gatgtacctg ctcaccaacg acgagaaatc
     5041 gcagctcggg acgacgccgt tgcccgacgg cgtcgtccgc gtcttccgcg acaatggcaa
     5101 gggcgggctc tccttcgtga cgcagcagtc gatcaagtac atcccgatcg gcgacaagat
     5161 cgagctgaac ctgggagtcg atccggaggt gatcttcgag ctgatcaagc tccgggcctt
     5221 ccgcagcgac ctgtggctgc aaatcaccgg cgcgaccgag ctgcggaaag tcggcggcga
     5281 cgtcgtcgtc aaacaggaga atgcctcgat cgtcggctgg gacgaccatg agctctacag
     5341 ccagagaatc cgcaactaca ccggcgacga aatcgaagtt gaaatcagac gctcgttcga
     5401 cgggcacgtc gttttccgga gccagctcga gcccaagctg cacgattatc ggacggccct
     5461 gttcctcgcg cgcattgctc ccggcaagcg agccgatttg ctcttcgaga tcgtccgcaa
     5521 gcaaggaacc agcgctaagc agaacaacat cacactcgaa gcagcggagg tcaagccgta
     5581 agtggctgat tgggttcctg ttcttgatct ccctcgtaat cgtaatccta ctcatactcc
     5641 tactcctgtc cgctggcggg cgggcaggag aaggagccag agcaagagaa ggaagaggag
     5701 caggattatg agtaagagca ggattaggat tctgtctatt ccggatagag ctgcgtcgag
     5761 agataccgct cacccaggtc gggcatgacc acgacgatca ccttgccggc attctcgggc
     5821 cgctgcgcga ccttgacagc cgccgccact gccgcgccag aggagatgcc gcagagcatc
     5881 ccttcctcct tggcgagccg ccggcccatc tcgaacgact catcgtccgt gacctgcacc
     5941 acatcgtcga tgactgcggt gttgagcacg tccgggatga acccggcgcc gatgccctga
     6001 atcttgtgcg ggccggcctt cagctggtcc ttgggcaccc cttgcatcag gtgctgggtc
     6061 aggacggcgc tggccgtcgg ctctaccgcg atgcacttca cgctcgcttt gcgcgccttg
     6121 agcacctcac cgcagccggt gatggtgccg ccggtgccga cgccggaaac gaggatgtcg
     6181 atttgcccgc tcgtggcgcg ccagatttcc tcggcggtgg tcttgcggtg gacctccgga
     6241 ttggccgggt tcttgaactg ctgggggatg aaggccttcg gctccccgcc gtgctcctgg
     6301 aacagctcca ccgcccggcc cacggccccg cgcatgccgg tctcgcgggg cgtcaggtag
     6361 agcttcgccc cgaacgcctt caggagc
//
LOCUS       DASUGB010001096         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2066902, whole genome shotgun sequence.
ACCESSION   DASUGB010001096 DASUGB010000000
VERSION     DASUGB010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066902"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFV87_20815"
     CDS             <1..1502
                     /locus_tag="VFV87_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5106280.1"
                     /translation="RGLRVPPWVKTSLAPGSRVVSDYLDKSGLTKELDKLGFQTVGYG
                     CTTCIGNSGPLPPPVAQAVTEGDLVAAAVLSGNRNFEGRVNPLVKANYLASPPLVVAY
                     AIAGTTDIDLVKEPIGKSADGTQVYLKDIWPTNEEVKQAQQTSVLPQMFASRYSNVFA
                     ANEVWNQIKVAAGDLYQWDEASTYIQEPPFLADMTVETGSIQPIAGARVLAVLGDSVT
                     TDHISPAGNISKSSPAGKYLIANGVQPADFNSYGSRRGNDRVMVRGTFANIRIRNFLA
                     PGTEGGVTKYLGPPSFNRVPKGSESGGAAAAAARHGMKEPATLPNGNARMIDEGGGSK
                     PAPMDTPEKGTDHPDIGEVVSIYDAAIAYKASGVPTIVLAGAEYGTGSSRDWAAKGTY
                     LLGVRAVIATSYERIHRSNLVNMGVLPLQFLEGQSWKSLGLTGEETYQIDSLSDSLQP
                     RSKLTVRAKAADGSEKKFETHVRIDTPVELDYYRNGGILQTVVRKLIAG"
     gene            1536..2081
                     /locus_tag="VFV87_20820"
     CDS             1536..2081
                     /locus_tag="VFV87_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106281.1"
                     /translation="MKSLGELSLVLLVAALLAGCDASGPAPTANAPTPPAPPAPPPPP
                     GTGGAPAAGAAAPKAPVPELQPYWGQNYIVFVFARDRHNKSYQDAKADWQAHRTDQEG
                     KPIMFVEFIGTISGETFDKLTFQVEGGPTISGKSASDLWDSMGASNSVTSILIIGKDG
                     KMIFDKRRGGDVDLAAALEPL"
     gene            2203..2580
                     /locus_tag="VFV87_20825"
     CDS             2203..2580
                     /locus_tag="VFV87_20825"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5106282.1"
                     /translation="MKVQNATDLAKKKCVPCEGGAEPLWPQEAQRQLAKLPGWSLGRA
                     GQRIGKTWVCKDFAAAMKFLNAIAKLAEQQDHHPDLHLTGYRQVEVEIYTHAVGGLSE
                     NDFILAAKIDRLPVEIKKPKKKK"
     gene            2655..3437
                     /locus_tag="VFV87_20830"
     CDS             2655..3437
                     /locus_tag="VFV87_20830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5106283.1"
                     /translation="MLQIKVGIRLASLRLPFRQALAKAKELGAEAVEIDARSELRPED
                     LSHTGVRQVRKILEDLALKVSAVSFHTRRGYDVLEDLEPRIDATKRTLQLAYDLGAGV
                     VVNHIGRVPDQLDSPDAATLLQALADIGQHGQRVGALLAAETGTESGAQLAQLIARLP
                     PGSIGVDLNPANLIVHGHEPREAARALGEHILHVHATDATRDVARKSGLEVPLGQGSA
                     EWPELLGILEEQQYRGYFTVARNAGGNALADIRQAVNFVRNI"
BASE COUNT          679 a         1130 c         1123 g          524 t
ORIGIN      
        1 cacgcggcct gcgcgtgccg ccgtgggtca agacaagcct cgcgccgggt tcgcgggttg
       61 tgagcgacta cctcgacaaa tcggggctca ccaaggaact cgataagctg ggattccaga
      121 ccgtgggcta cggctgcacg acgtgcatcg gcaacagcgg tccgctgccg ccgccggtgg
      181 cccaggcagt gaccgagggg gatcttgtcg cggcggcggt gctctcgggc aaccgcaatt
      241 tcgaggggcg cgtcaatccg ctggtgaagg cgaattacct ggccagcccg ccgctcgtcg
      301 ttgcctacgc catcgccggc acgaccgata tcgacctggt caaggagccg atcggcaagt
      361 ccgccgatgg gacgcaggtc tatctgaagg acatctggcc gacgaatgag gaggtgaagc
      421 aggcccagca aacgtcggtc ctgccgcaga tgttcgcctc gcgatattcc aacgtgtttg
      481 ccgcgaacga ggtctggaac cagatcaaag tggccgcagg cgatctctat cagtgggacg
      541 aggcgagcac gtacatccag gagccgccgt ttctggccga catgaccgtc gagacgggga
      601 gcatccagcc aattgccggg gcccgcgtgc tggcagtgct gggcgacagc gtgacgaccg
      661 accacatctc gccggccggc aacatcagca agtcgagccc cgcggggaag tacctgattg
      721 ccaacggcgt gcagccggcc gatttcaaca gctacggcag ccggcggggg aacgaccggg
      781 tgatggtccg cggcacattc gcgaacatcc ggattcgcaa tttcctcgcc cccggcaccg
      841 aagggggcgt gacgaagtac ctgggtcccc cctcgtttaa ccgcgtcccg aagggaagcg
      901 agtcgggcgg cgcagccgcc gccgcggccc gccacggcat gaaagaaccc gccacgcttc
      961 ccaacggcaa cgcccggatg atcgacgaag ggggcggatc gaaacccgcg ccgatggata
     1021 cgcccgaaaa agggacggac catcccgata tcggcgaagt cgtctcgatc tacgacgccg
     1081 ccatcgccta caaggcctcg ggagtgccga cgattgtgct ggccggcgcg gaatatggca
     1141 ccggctcgtc gcgcgactgg gccgccaagg ggacgtacct gctgggcgtg cgggcggtca
     1201 tcgcgacgag ctacgaacgc atccaccgca gcaacctggt caacatgggc gtcttgccgc
     1261 tgcagttcct ggaggggcag tcgtggaaat cgctcggcct caccggcgaa gagacctacc
     1321 agatcgacag cctgagtgat tccctccagc cgcggtccaa gctcacggtc cgcgccaagg
     1381 ccgccgacgg cagcgaaaag aagttcgaga cccacgtgcg aatcgacacg ccggtcgagt
     1441 tggactacta caggaacggc gggattttgc agacggtggt gcggaaactc atcgccggat
     1501 aaatggcgag ttcgctgcat gttcaaggag ttgaaatgaa atcgcttgga gaattgtcgc
     1561 tcgttttgct ggttgccgct ctcctggccg ggtgcgatgc ttccgggccg gcgccgaccg
     1621 cgaatgctcc cacgccgcca gcgccaccgg cgcctccgcc gccccctggc acgggcggtg
     1681 cgccggctgc cggggcggca gcgcccaagg cacccgttcc ggaactgcag ccgtactggg
     1741 gccagaacta catcgtgttc gtgttcgcgc gcgatcgtca caacaagagc taccaggacg
     1801 ccaaggccga ttggcaggcg catcgcaccg accaggaagg caagccaatc atgttcgtcg
     1861 aattcatcgg caccatctcg ggcgagacgt tcgacaagct cacgttccag gtggaaggcg
     1921 gtcccacgat ctccgggaaa tcggcgagcg acctgtggga cagcatgggg gccagcaaca
     1981 gcgtgacatc gatcctgatc atcggcaaag acggcaagat gatcttcgac aagcgccgcg
     2041 gcggcgacgt ggacctcgca gcggcgctgg aaccgctcta aatcgcccgc cacgggccga
     2101 ttggcgcagc gctttcgccc gctatgctag gccgtacagt agcgcttgct cactcagtag
     2161 cggctggtca cacaggctgt ccaactcctc accgccttac gcatgaaagt ccaaaacgcc
     2221 actgacctgg ccaaaaagaa atgcgttccc tgcgaagggg gcgccgagcc gctctggccg
     2281 caagaggccc agcgccaact ggcgaagctg cccggctgga gcctgggccg ggccggccag
     2341 cggatcggca agacctgggt ctgcaaggac ttcgcggcgg cgatgaagtt tctcaatgcg
     2401 atcgccaagc tcgccgagca gcaggatcat catcccgatc tgcacctgac cggctaccgg
     2461 caggtagaag tcgagattta cacccacgcc gtcggcggcc tgagcgagaa cgatttcatc
     2521 ctcgccgcca agatcgaccg gctgccggtg gagatcaaga agccaaagaa gaagaagtag
     2581 gacgggtatt ccaatccgtc cgataaagga cagattacaa acctgtccta tgcctacgcg
     2641 atgaggaggc gctcgtgctg cagatcaagg tgggcatccg cctggctagc ctgcggctgc
     2701 cgtttcgcca ggccctcgcg aaagccaaag agctgggggc cgaggccgtc gagatcgatg
     2761 ctcgcagcga gctgcgcccc gaagacctct cgcacacggg cgtgcggcag gtgcggaaga
     2821 ttctcgaaga cctggctttg aaggtcagcg ccgtctcgtt ccacacgcgc cgcggctacg
     2881 acgtgctgga agacctggag ccgcggatcg acgctacgaa gcgcacgctg cagctcgcct
     2941 acgacctggg cgccggcgtc gtcgtcaatc atatcggccg cgttcccgat cagctcgatt
     3001 cgccggatgc cgccacgctg ttgcaggccc tggcggacat cggccagcac ggacagcgcg
     3061 tcggcgcgct cctggcggct gaaacgggga cggaaagcgg cgcgcaactg gcgcagctca
     3121 ttgcccgcct gccgccgggc tcgatcgggg tcgatctgaa cccggccaac ctgatcgtcc
     3181 acggccacga gccgcgcgaa gcggcccgcg ccctgggcga gcacatccta cacgtccacg
     3241 cgaccgacgc cacgcgcgac gtcgcccgca aaagcggcct ggaagttccc ctcggccagg
     3301 gcagcgccga atggccggag cttttgggca ttctggaaga gcagcaatac cgcggctatt
     3361 tcaccgtcgc gcggaatgcc ggcggcaatg ccctcgccga tatccgccag gcggtgaact
     3421 tcgtgcggaa tatctgacca cgtgctggtt cccacg
//
LOCUS       DASUGB010001097         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_120159, whole genome shotgun sequence.
ACCESSION   DASUGB010001097 DASUGB010000000
VERSION     DASUGB010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120159"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..393)
                     /locus_tag="VFV87_20835"
     CDS             complement(67..393)
                     /locus_tag="VFV87_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106284.1"
                     /translation="MATRNMINAGRKRSVSPRQAIKVAVVALAIAAFGAAGLSGALAK
                     HGADDWSESVFGSGSSGGSGWDDQFASGGSGGSGWDDDDIFASGGSGSGGWDDDSIYS
                     SGGWDD"
     gene            725..940
                     /locus_tag="VFV87_20840"
     CDS             725..940
                     /locus_tag="VFV87_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106285.1"
                     /translation="MSFATDVKPLFREQDREAMDFAFDLWSYDDVKENAGVILDRLED
                     GTMPCDEPWSEEKLQTFRDWIVAGSPR"
     gene            1267..>2635
                     /locus_tag="VFV87_20845"
     CDS             1267..>2635
                     /locus_tag="VFV87_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106286.1"
                     /translation="MLDGAAPLLTMTGPGGVGKTQLALVVGARVGDSFGDGVAFVDLS
                     SISDPTLVFTAVALAIGLVVGPRREPAAQLVAHLRSRQFLLILDNCEHLTDTVAGLVS
                     KLLSACPALQVLVTSRSTLRIRGERTFPVPPLAVPDGASTGWSDAAQAPAVALFTQRA
                     QAVRPEFTLDEANAAVVAEICRRLDGLPLAIELAAARVVLLSPVALLDRLNSRLRLLT
                     HAARDAPKRHQTLRAAVAWSYDLLSPEEQQLFRRLAVFVGGFPLEAAEAVGGGESTTP
                     SAPSVVDLLASLGEQSLLSSPTVVGGGDRVAMLETVREFALETLVASGEAQRARYAHG
                     AYFLGLAEEAEPHLTGKDQIQWLNRLEAEHANLHAALEFFAGQGMTEQALRLAGALGG
                     FWWRHGHFHEGRQWLERLLAVEEAHPGLTSEGVWAKAFTVAGILAWTRGDFERAAVCH
                     ETALRG"
BASE COUNT          418 a          816 c          899 g          502 t
ORIGIN      
        1 agaaaccgtg actcgggaac gggccctgtt cccgagtcag acccgcggca agagggtttg
       61 cggatgctag tcgtcccagc caccactgct gtagatcgag tcgtcgtccc agccgccgga
      121 accgctgccg ccgctggcga agatgtcgtc atcgtcccaa ccgctgccgc cgctcccgcc
      181 actggcgaat tggtcatccc agccggaacc accggagctg ccgctgccga agacggactc
      241 gctccagtcg tctgcgccgt gcttggccag tgcaccggaa agcccggccg cgccgaacgc
      301 ggcgatggcg agcgcgacga ccgcgacctt gatcgcctgc cgtgggctga cgctccgttt
      361 ccttcccgcg ttgatcatgt tcctggtcgc catggaccgc tcctttcccc gtcatgtctg
      421 cggcacgccg actcgctgtg cctgacatga cgcgccatct cgataggttg ccgggccgtc
      481 gaccggtggc gggtcgtccg cgaccgagaa gaccggcccg ccagcttcgg cgctggcagt
      541 cacgagtgtg ctccacgcgg ggccggtcgc gaatcggacc gcagtcggga atcgatatcg
      601 gactttggtc ccactggcgc agggggaacg cgagccgcac ggacggaatc ctgcgacaac
      661 gctggatgcg ggtgaggaga ttggtacact cgccgcgcga ttggtcaggc tgggggagcg
      721 tggaatgagc ttcgcgaccg acgtcaaacc gctgtttcgc gagcaggatc gcgaggcgat
      781 ggacttcgcc ttcgatctct ggtcgtacga cgatgtgaag gagaacgcgg gggtgattct
      841 cgaccggctc gaggacggta cgatgccatg cgacgagcca tggagcgagg agaagctgca
      901 aacctttcgg gactggatcg tggccggctc tccgcggtag cgggagcggg tgccgactgg
      961 tcactcaggg aagggttttc agcccgaacc gctggagcct ggcggccgaa actcgtatgt
     1021 gctggccact ctctctgact cgttaccccg tcgaggcgcc gggccagatc aggtgtggct
     1081 ggcagcgatg ctggcttgac ggacaattga tggccacagt atttatgtgc agcgattgag
     1141 gtccaacgat aaacgttccg gatggtgatg gcagcaccgg cgaggtggct cggctccagc
     1201 atacttccgc tcccgcggac cccaatagtg ggccgaacgc gggagatagc tgatgtcgtc
     1261 cggctcttgc tcgatggcgc ggcgccattg ttgacaatga cggggccggg cggtgtgggc
     1321 aagacccaac tggcactggt ggtcggcgct cgtgtcggcg acagcttcgg ggacggagtt
     1381 gccttcgttg acttgtcctc gatcagcgat cccacgcttg tgttcactgc cgtggcgctg
     1441 gcaattggtc ttgtcgttgg ccctcgtcgt gagccggctg cgcaactcgt cgcgcatctc
     1501 cgttcccggc aattcctgct gatcctcgac aactgcgagc atctgaccga cacggtggct
     1561 gggttggtgt cgaagctgct ctcggcatgc ccggccttgc aagtgctggt caccagccgc
     1621 tcaacccttc gcatccgcgg cgaacggaca ttccctgtcc cgccacttgc ggtgccggac
     1681 ggggcaagca caggatggtc cgacgccgcg caggcgcctg ccgttgcgct cttcacgcag
     1741 cgagcgcagg ccgttcggcc cgagttcacg ctcgacgaag cgaacgccgc ggtcgtggcc
     1801 gaaatctgtc gccgtttgga cggtctgccg ctggccatcg aattggccgc cgcgcgcgtt
     1861 gtccttctct caccggttgc gctgctcgat cggctcaaca gtcggctgcg tctgctcacg
     1921 catgcggcgc gcgatgcgcc gaaacggcac cagaccttgc gcgccgctgt cgcttggagc
     1981 tatgacctcc tctctcctga agagcagcag ctctttcgac ggctggcggt ctttgtcggt
     2041 ggtttcccgc ttgaggctgc ggaggcggta gggggaggcg aaagcaccac tccctccgct
     2101 ccatctgttg tcgatctcct cgcgtcgttg ggagaacaga gcttgctctc gagcccaacg
     2161 gtggttggag gcggcgatcg ggttgccatg ctggagactg tgcgagagtt tgccctggag
     2221 acgcttgtcg ccagcgggga ggcacagcgg gcgcgatacg cgcacggcgc gtattttctg
     2281 ggtcttgccg aggaggcgga gccacatctg accggcaagg atcagattca gtggctcaac
     2341 cgtctcgagg cggagcacgc caacctgcat gccgcgctcg agttcttcgc cggacaggga
     2401 atgacagagc aggcgctgcg actggcaggg gcgctcggtg gcttctggtg gcgacatggg
     2461 catttccacg aaggacggca gtggctggag cgcctccttg ccgtcgagga ggcgcatccg
     2521 gggcttacct cggaaggcgt gtgggccaaa gcgttcactg tggcaggtat cctggcgtgg
     2581 acacgaggtg attttgaacg ggctgcggtc tgtcatgaaa cggcactgag gggct
//
LOCUS       DASUGB010001098         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2067154, whole genome shotgun sequence.
ACCESSION   DASUGB010001098 DASUGB010000000
VERSION     DASUGB010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067154"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(501..2141)
                     /gene="pgm"
                     /locus_tag="VFV87_20850"
     CDS             complement(501..2141)
                     /gene="pgm"
                     /locus_tag="VFV87_20850"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX5106287.1"
                     /translation="MALHPLAGKPAPADMLVHLARLTRAYYDGSPDLADPQQLVSFGT
                     SGHRGSSLNGTFTEAHILAISQAICEYRKSQGTDGPLYMGQDTHALSGPAQRTALEVL
                     AANEVVVHLQRDEAPTPTPVISRAILVHNRGRERGLADGIVITPSHNPPEDGGFKYNP
                     TSGGPADTEVTRWIQNRANELLRGGNREVKRVRYERAIAASTTIEEDFVIPYVNDLAT
                     AIDVDAICSSGLKIGVDPLGGASIHYWEPIAAEYGLNIEVVNRRIDPTFSFMTVDHDG
                     KIRMDCSSPYAMASLVRLKDQYQVAFAADPDSDRHGIVTPSLGLMNPNHYLAVAIRYL
                     LTSREKWPKSAVVGKTLVSSGLIDLVVASLGRTLWEVPVGFKWFAPGLFDGTCCFGGE
                     ESAGASFLRIDGTVWTTDKDGILLDLLAAEITARTGKDPGQHFQELVGQFGQPHYTRI
                     DAPATPDQKAKLAKLSPEAVTAEKLAGEPIVAKLTKAPGNGAAIGGLKVVSQGGWFAA
                     RPSGTENIYKIYAESFVSGRHLDDIVAAAQQIVADALS"
     gene            complement(2158..>2550)
                     /locus_tag="VFV87_20855"
     CDS             complement(2158..>2550)
                     /locus_tag="VFV87_20855"
                     /inference="COORDINATES: protein motif:HMM:NF017806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HEX5106288.1"
                     /translation="GYGGALIREKIVAAASRKLVILVGEEKLVPALGSRGNIPVEVVP
                     FGLAATAAHLRRLGLNPQQRMAGGQPVITDNGNMILDCGTQPIAHPAELEAAIVAIPG
                     VVGTGMFLGMADVVLVQRGERVEELKRP"
BASE COUNT          430 a          793 c          850 g          477 t
ORIGIN      
        1 tctcgtccgt gatgcggtgc tttgaatagt cgagatagat gccttccgct tcgagcgcca
       61 tgcgctcgcc gcgcgaggca tcgtcggcaa agagctgccg caggtgcagg tccttgatct
      121 gctgaaagtg cgtttcaaga gccttccaag caggcaacag gcgaaggggg gtggtcctag
      181 ggtcttgggc ttcgagatgc acgtcgactg cgaaagttgt ccgcggaagg ggtcgggagt
      241 caattgcggt cacggcgctc catttggata acgtctgccg ccattgactc ccgaccccgt
      301 tggcgtttca agggccttcc aagcgggcaa ctggcgaagg ggcgtggtca tagcttttct
      361 aagccgggac caaacttggt cccggcgtac gtggggcagg cttccagcct gccaaagagg
      421 aacggtgatc aatgggaatg gcgagtcata tcgtggcagg caagatgcct gccccacgcc
      481 gggagcaagt ttgctcccgg ctaggaaagg gcgtcagcca caatctgctg cgccgcggcg
      541 acgatgtcgt ccaggtgccg accgctgacg aagctttccg cgtaaatctt gtagatgttt
      601 tcggtgccgg agggtcgggc ggcgaaccag cctccttggc tgacgacctt caacccgccg
      661 atcgccgctc cgttgccggg cgctttcgtc agcttcgcca cgatcggctc tccggcgagt
      721 ttttccgccg tcaccgcctc gggcgacagt ttcgcgagct tcgccttctg gtcgggggtg
      781 gccggggcgt cgatgcgggt gtagtgcggc tggccgaatt ggcccaccaa ctcctgaaag
      841 tgctggcccg gatctttgcc ggtgcgggcc gtgatctccg ccgccagcag gtcgagcagg
      901 atgccatcct tgtcggtggt ccaaaccgtg ccgtctatcc gcaggaagct ggccccggcg
      961 ctctcttcgc cgccgaagca gcaggtgccg tcgaacagtc ccggtgcgaa ccacttgaag
     1021 ccgaccggca cttcccatag cgtgcggccg aggctggcga cgacgagatc gatcagcccg
     1081 ctggaaacca gcgtcttgcc gaccaccgcc gatttcggcc acttctcgcg gctggtgagc
     1141 agatagcgga tggcgaccgc caggtaatga ttcgggttca tcagccccag cgagggtgtc
     1201 acgatcccgt gccggtcgga atcggggtcg gcggcaaagg ccacctggta ctggtccttc
     1261 agccgcacca ggctggccat cgcatagggg ctggagcaat ccatgcgaat cttgccgtcg
     1321 tgatcgaccg tcatgaacga aaaagtgggg tcgatccttc gattgacgac ttcgatgttc
     1381 aggccgtact cggcggcgat cggctcccag taatgaatcg aggccccgcc gagcgggtcg
     1441 acgccgatct tcaggcccga gctgcaaatc gcatcgacgt cgatcgccgt tgccaggtcg
     1501 ttcacgtacg gaatgacgaa atcctcctcg atcgtcgtcg aggcggcgat cgcccgctcg
     1561 taccgcacgc gtttcacctc gcgattgccg ccgcgaagca gctcattggc ccggttctga
     1621 atccagcggg tgacttcggt atcggccggg ccgccgctgg tggggttgta cttgaagccg
     1681 ccgtcctccg gcggattatg cgaaggagta atgacgatgc catcggccag accgcgctcg
     1741 cggccgcggt tatgaacgag gatggcccgc gaaatgaccg gcgtcggcgt aggggcttcg
     1801 tcgcgctgca gatggacgac gacctcgttg gcggcgagga cttccagggc cgttcgctgg
     1861 gccggtccgg aaagggcgtg cgtgtcctgg cccatgtaga gcgggccatc ggtgccctgg
     1921 ctcttgcggt attcgcagat cgcctgcgaa atcgccagga tgtgggcctc ggtaaacgtg
     1981 ccattcagcg acgagccgcg atggccgctg gtgccgaagc tgacgagctg ctgcggatcg
     2041 gccaggtcgg gcgagccgtc gtaataagcc cgcgtcagcc gggccagatg gaccagcata
     2101 tcggccgggg cgggctttcc tgccagggga tgcagtgcca tgggtgcctc tcacgggcta
     2161 gggacgtttc agctcttcga ctcgctcgcc gcgctgcacg agcacgacat ccgccatacc
     2221 caggaacatt cccgtcccca cgacgccggg aatcgcaaca atggcggctt ccaactccgc
     2281 gggatgggcg atcggctgtg tgccgcaatc gaggatcatg ttgccgttgt cggtgatgac
     2341 cggctggccg cccgccatgc gttgctgggg attgaggccc agccgccgca aatgcgctgc
     2401 cgtcgcggcc aggccgaacg gcaccacttc gacgggaata ttgccgcgcg aaccgagcgc
     2461 cgggacgagt ttttcctcgc cgacgagaat gacgagcttg cgcgaagcgg ccgcgacgat
     2521 cttctcgcgg atcagcgctc cgccgtagcc
//
LOCUS       DASUGB010001099         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1509720, whole genome shotgun sequence.
ACCESSION   DASUGB010001099 DASUGB010000000
VERSION     DASUGB010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509720"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..2982
                     /locus_tag="VFV87_20860"
     CDS             88..2982
                     /locus_tag="VFV87_20860"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX5106289.1"
                     /translation="MSFFSPRSSFAPLVAALVLVGFAALLTGCGAEAGPVTAASSEFR
                     PAGEGASETAPDASDVARNTASEDISSDRTQFSPSTANAASNAPKAKQPPAKSGSAAS
                     APPQGDVETLVAFIDKLASQQPQGSSQEEIAADFIRIQMARLDAAKKILALNPDDRTK
                     QGAIKVALEILSIFSQAQVPGADAQRLAFGKLLLNDKNPDTARMGRHIVFGNNVGNML
                     NRGLKSGDEVVAQVKELLATEGDEVSDPAMALTRQTAQALRDQGFTDDAVAVIDLLVA
                     KLENDPERADEAAALKDQAKIAQADLTGDLLVNVMTGEAGSEEKLIEAVKKLLAEASP
                     SAGIFGEVQQVAYRLEVIGKNDAALKTLELIESAYKDVGEKRIADAVSETLKNARTRI
                     GLIGQPFVVEGLTLDGQPFDWEAYKGKVVLVDFWATWCGPCIEELPNILRNYKDFHDL
                     GFDVVGVNMDTDLEQVKQFLSLQELPWTSVTSQPVIDGSAEEPWLLPMPKKCGVDGIP
                     FLVLVGKDGKVDSLHTRGPRLKTRLTQLLGEPPAPKPVDVPTDPTAPAKPATEKPADE
                     KPAEKPAAEEKPAEQGARVQPALSPVALLVAQALLTADEPATTEKPAEPIPAAEDKSI
                     NPYKAKPGLSTAQLIDYIERMFDKPKSIRGREGFTEAVVDACDRILAADPPAKEALQL
                     VAIESKCEALHRKACTGDDACDKDLMEFVTSLKDDQRPRVARQVDFYQRERRVLDAVE
                     GPIEEIPPLLEELKKYYESEKLTARHLRMASSTVGLINRLEDGDQREEHFAAFGGAFA
                     KAGDKELARYGKKLAKKPGAVESDLVGQPLELAGTTAKGADFVWDAYRGKVVLVDFWA
                     TWCGPCRREMPGVKAFYEKHVDQGFDVVGVSLDQDQEALAQYLDENQIAWDTLAGDDT
                     QELASKYGVRGIPTMMLVDKDGKVVGVSHSLAALAPLAEKLLGAQAAEGK"
     gene            2995..4023
                     /locus_tag="VFV87_20865"
     CDS             2995..4023
                     /locus_tag="VFV87_20865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5106290.1"
                     /translation="MDVSSPADAPLAPRRAIPAWLLSLACHLAAVVLGCLLIRGAPPP
                     KGIAEADRPAGIVLVQRAANQAQYFSDDDRHEALKPAAATAAANPGGSTTAKLLPASE
                     PPLIAGIDLPSLPGAVVGGEGLVTRVTPGTGRGKPLVLPGLDDAAILAEDAAIPREAQ
                     ATGPTAQLSLFGSAAAEGRSFVFVIDRSQSMGANGLGVLGAAAEELTAQLGRLTSQQT
                     FEVVAYNQSIATFAGRELVAADDKNKDRLVRFVANMAAYGQTEHLRGLVAALKLKPEV
                     IFLLTDGGDPVLDNVQLRTIRDLAAGRTSIHTLHFGRGAAPEGENFLQRLAAENRGSY
                     VYIDMNRR"
BASE COUNT          813 a         1414 c         1372 g          679 t
ORIGIN      
        1 cctttcgcct agaatgacgc cagagaactt acccgctcgc ccggcttttc ccgccggcac
       61 gctatttctt ctgcaaaggc cgctgccatg tcctttttct ctccgcgctc ctcgtttgca
      121 cctctcgtgg cggccttggt cctggtcggc tttgcggctc tcctcaccgg atgcggtgcc
      181 gaagccggac cggtcacggc ggcctcgtcg gagttccgcc cagcgggtga gggagcttcc
      241 gagaccgctc ccgacgcgag cgacgtggcg cggaatactg cctcagagga catcagttcg
      301 gaccgaaccc aattctcccc atccaccgcc aatgcagcct cgaatgcccc gaaggcaaaa
      361 cagccccccg ccaaatccgg ctccgctgct tcagctcctc cccagggcga tgtcgaaacg
      421 ctcgtggcct tcatcgataa actcgccagc cagcagcctc aaggctcgtc gcaagaagag
      481 attgccgcgg acttcatccg cattcagatg gcccgtctgg acgccgccaa gaaaatcctg
      541 gcccttaatc ccgacgaccg caccaagcaa ggagccatca aagtcgccct cgaaatcctg
      601 agcatcttca gccaggccca ggtgcccggc gccgacgccc agcggctggc gttcggcaag
      661 ctcttgttga acgacaagaa tccagacacc gcccgcatgg gccggcacat cgtgttcggc
      721 aacaacgtcg gcaacatgct caatcgaggt ctgaagagcg gcgacgaagt cgtggcgcag
      781 gtcaaggaat tgctggcgac cgaaggggac gaggtcagcg acccagcgat ggcgctcacc
      841 cgacagactg cccaggctct ccgcgaccag ggattcaccg acgacgccgt ggccgtcatc
      901 gacctgctcg tggccaagct cgagaacgat ccggagcgcg ccgacgaagc cgccgccttg
      961 aaggaccagg ccaagatcgc tcaggccgat ctgaccggcg atttgttggt gaatgtgatg
     1021 accggcgagg ctggctcgga ggagaagctg attgaagcgg tgaagaagct gctggccgaa
     1081 gccagtccga gcgccggcat cttcggcgaa gtgcagcaag tggcctatcg tttggaggtc
     1141 atcggcaaga acgatgcggc cctgaagacg ctggagctga tcgaatccgc gtacaaggac
     1201 gtgggggaaa agagaattgc cgacgccgtg agcgagacgc tgaaaaacgc ccgcacgcgg
     1261 atcggcctga ttggccagcc gttcgtcgtg gaaggtctga cactggacgg gcagcccttc
     1321 gattgggaag cctacaaggg caaagtcgtg ctggtcgatt tctgggccac ctggtgtggt
     1381 ccctgcatcg aggagctgcc gaatatcctc cgaaattata aggattttca cgacctcggc
     1441 ttcgacgttg tgggcgtgaa catggatacg gacctggagc aggtcaagca gttcctctcg
     1501 ctgcaggagc tgccctggac gagcgtcacc agccagccgg tcatcgacgg ctcggccgag
     1561 gagccctggc tcttgccgat gcccaagaag tgcggcgtcg atggcattcc gttcctcgtc
     1621 ctcgtgggaa aagacggcaa ggtcgattcg ctgcacaccc gcgggccgcg gctgaagacg
     1681 cggttgacgc agcttctggg cgagccgccg gctcccaagc cggtggacgt tcccaccgat
     1741 cccaccgcgc cggccaagcc ggccacggaa aaacccgctg atgaaaaacc agccgaaaaa
     1801 cccgccgcgg aggaaaagcc ggcggagcag ggcgctcgcg ttcagccggc attaagtccg
     1861 gtcgccctgc tcgtggctca agcgctgcta accgccgacg aacccgccac caccgagaaa
     1921 ccggccgagc cgattcccgc cgccgaagat aagtcgatca acccctacaa ggccaaaccc
     1981 ggtctctcga cggcccagct catcgactac atcgagcgga tgttcgacaa gcccaaaagc
     2041 atccgcggcc gcgaaggctt taccgaagcc gtcgttgacg cctgcgaccg gattttggcc
     2101 gccgatccgc ccgcgaagga agccctccaa ctcgtcgcga tcgaatcgaa gtgcgaagcc
     2161 ctgcaccgca aggcctgcac gggcgatgat gcgtgcgaca aagacctcat ggagttcgtc
     2221 acatcgctca aggacgacca gcggccgcgc gtcgcccggc aggtcgattt ctatcagcgc
     2281 gagcgacgcg tgcttgacgc cgtcgaaggg cccattgagg agattccgcc gctgctcgag
     2341 gagctgaaga agtactacga gagcgaaaaa ctgactgccc gccatctgcg gatggcctcc
     2401 agcacggtcg gcctgattaa ccggctggag gacggcgacc agcgggagga gcattttgcg
     2461 gcgttcggcg gcgcgttcgc caaagctggc gataaggagc tggcccgcta cggcaagaag
     2521 ctggccaaga agccgggggc ggtcgaatcg gacctcgtcg gccagccgct ggagctggcg
     2581 ggcacaaccg cgaagggggc agatttcgtg tgggacgcct atcgcggcaa ggtggtgctg
     2641 gtcgattttt gggccacctg gtgcggcccc tgccgccgcg aaatgcccgg cgtgaaggcg
     2701 ttctacgaga agcacgtcga tcagggcttc gatgtcgtcg gcgtcagcct cgatcaggat
     2761 caggaggcgc tggcccagta tctggacgaa aaccagatcg cctgggacac actggccggc
     2821 gacgacacgc aggagctggc gagcaaatac ggcgtccgcg gcattccgac gatgatgctc
     2881 gtcgataagg acggcaaggt cgtcggcgtt tcgcacagcc tggccgcgct cgcgcctctg
     2941 gccgaaaagc tgctgggtgc tcaggcggcg gaagggaaat gagacttcgc cgcgatggac
     3001 gtcagcagcc ctgccgatgc gccgctcgcc ccacggcggg caattcccgc gtggctgctg
     3061 tcgctggcgt gccatctggc ggcggtcgtg ctgggatgct tgctgatccg cggagcgccg
     3121 ccgcccaagg gcatcgccga agccgatcgc ccggcgggga tcgtgctggt gcagcgcgcg
     3181 gcgaatcagg cgcagtactt cagcgacgac gaccggcatg aggcgctcaa gcccgcggcc
     3241 gcaactgcgg cggccaaccc cggcggcagc acgactgcga aattattgcc cgcgagcgag
     3301 ccgccgctga tcgccgggat cgacctgccg agcctgcccg gcgcggtggt cggcggcgaa
     3361 gggctcgtca cgcgcgttac gcctggcacc ggccgcggca agccgctcgt tctgccgggg
     3421 ctcgatgatg ccgcgatcct ggcggaagac gccgccatcc cgcgcgaggc gcaagcgacc
     3481 ggtccgaccg cccagctctc gctcttcggc tcggctgcgg cggaggggcg gtccttcgtg
     3541 ttcgtgatcg accgctcgca gagcatgggg gcgaatggct tgggcgttct tggcgccgct
     3601 gccgaggagc tgaccgccca gcttggccgc ctgaccagtc agcagacgtt cgaagtagtg
     3661 gcttacaacc agtcgatcgc tacgttcgcg ggccgcgagc tggtcgctgc cgacgacaag
     3721 aacaaggacc gcctggtccg cttcgtcgcc aacatggccg cctatggcca gacggagcac
     3781 ctgcgcggcc tggtggcggc tctgaagctc aagcccgagg tgatctttct gttgaccgac
     3841 ggcggcgatc cggtgctcga caacgtgcag ctccgcacga ttcgcgacct ggccgcaggc
     3901 cggacgagca tccacacact gcacttcggc cgcggcgccg cgccggaggg cgaaaacttc
     3961 ctgcagcgcc tcgccgccga aaaccgcggc agctacgtct atatcgacat gaaccgccgc
     4021 tgaccgaagg ggtcgggagt caattgcggt catcaattga ccaaatgaaa acgcaagccg
     4081 caattgactc ccgaccccct ctaggttgcc ccgcgatgac gccctccatc cgcccacacg
     4141 agcagcgtct gatcgctctt cttcccgaac tgcaagcggc ccgagtcctg acgaacaccg
     4201 ccggccgcgg gcaattcgcg gcagcctatg cccgctcgcg gccggaagcg agcgtgacgt
     4261 gctggtttct cgatttct
//
LOCUS       DASUGB010001100         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_678025, whole genome shotgun sequence.
ACCESSION   DASUGB010001100 DASUGB010000000
VERSION     DASUGB010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678025"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFV87_20870"
     CDS             complement(<1..276)
                     /locus_tag="VFV87_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106291.1"
                     /translation="MAGRGLGQLFARKSVEQMHAEHQGSELKKSLGALNLVLLGIGCI
                     IGTGIFVLTGRAAAEFAGPGIMISFVITGILCALVALCYSELAAAIPV"
     gene            complement(427..927)
                     /locus_tag="VFV87_20875"
     CDS             complement(427..927)
                     /locus_tag="VFV87_20875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5106292.1"
                     /translation="MGLESVSALILDDNAHMRGLLRTILLGFGLRRIEEAQTSREALG
                     IAAGGDIDIAFVDFKLGGQDGLEFCRRIRTGEDSPDIYLPLVMVTAYSERRRVLDAIN
                     AGVDEFLVKPVRAVDVANRVNAIIERRRPFVQCGSYFGPDRRRRDDPRHTGPWRRAGD
                     PGTVDI"
     gene            1155..2615
                     /gene="rfaE1"
                     /locus_tag="VFV87_20880"
     CDS             1155..2615
                     /gene="rfaE1"
                     /locus_tag="VFV87_20880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX5106293.1"
                     /translation="MSPTPDQIVKLASGRVLVVGDVMVDHYVTGRVSRVSDEAPVPII
                     HVSGERWTPGGAANVAANIASLGGGAVLVGVVGKDAAATTLSDLLAPLAESARADLVV
                     EAGRPTTIKTRYMGGQHQIVRVDREEPAPISSETEAALVAAIEKHARDCQAVTISDYA
                     KGALTDRVIKAAIHAARSAGVPVVVDPKRADWTAYGGATVITPNRKELQLASHVPCSS
                     DDDCARAAGFAIKATRAAILLTRSEHGMSLYRDGKPPVSLAAKAREVFDVSGAGDTVA
                     ASVALGLASGLELEAAMQVANTAAGIVVGKRGTATVSPAELLAETGGGRGLGGSPGAV
                     PLAEARKLREKWGRDGLAVGFTNGCFDILHAGHIALLKQAAGACDRLVVALNTDASVR
                     GLKGPGRPVQSEQVRAAVMAAIKGVDVVVLFGEETPLEVIRELQPDVLIKGADYAEDQ
                     IIGADIVRARGGRIVRVTLVDGQSTSSVIERSKRGA"
     gene            complement(2637..>2906)
                     /locus_tag="VFV87_20885"
     CDS             complement(2637..>2906)
                     /locus_tag="VFV87_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106294.1"
                     /translation="GGDIVSSVARAASQEVDEAAAREPAVNREAADETLSERRAEAAR
                     KLTILSAFITAASLLAGAAAAFYAAGVGGKHRDQNTQLAFFVIRR"
BASE COUNT          522 a          976 c          927 g          481 t
ORIGIN      
        1 aaccgggatg gcggcggcaa gttccgaata acagagcgcg acgagggcgc acagaatgcc
       61 ggtaatgacg aaggagatca tgatccccgg gccagcgaat tccgcggccg ccctgcctgt
      121 cagaacgaag atgccggtgc cgatgatgca gccgatgccg agcaggacga gattaagggc
      181 gcccaatgat ttcttgagct cgctgccctg gtgctcagcg tgcatctgct caaccgactt
      241 ccgggcgaac agttgcccca gtccacgacc tgccattcga atgaccctcc gccgcgggat
      301 ctctcgggcc ccgccagttt gagccgggac gctagccgga cccgtcgaaa gcggcaaccg
      361 gcccctgttt acctttcacc agggtgggtt ttgcgcctgc gaacgtggca aaggggcaga
      421 gcctgctcag atatcgaccg tgccggggtc gcccgcgcgg cgccacgggc cggtatgtcg
      481 cgggtcgtcc cgccgccgtc ggtccggtcc aaaataactc ccgcactgca cgaagggccg
      541 cctgcgctcg atgatggcgt tgacccggtt ggccacgtcg accgcccgca caggtttcac
      601 aaggaactcg tccacgccgg cattgatcgc gtcgaggacg cggcggcgtt ccgaataggc
      661 ggttaccatc accagtggca aatagatgtc cgggctgtcc tcgccggtgc ggatacgccg
      721 gcagaattcc agcccgtcct gcccgccaag cttgaagtcg acaaaggcga tgtcgatatc
      781 tccgcccgca gcgatgccca gcgcttcgcg gctggtctgt gcttcctcga tccggcggag
      841 gccaaagccc agcaggatcg tccgcagcaa cccgcgcatg tgcgcgttgt cgtcgagaat
      901 cagcgccgat acgctttcga gacccatacc gtccaacgtc tgcccacagg cagccgctcg
      961 ccaaccccgt ccgagcgatt tccccggtgc ggcccttcag ccgcttgttc cctgcccccg
     1021 gcaaactccc ccagattcga gacaagtgca attatgcagg atagcgtctg gattgcttat
     1081 gatttgcgat agccgggccg tgacgcgacg agcggccttc gctagggaac actccagctt
     1141 cgacggacaa gcgcttgagc ccgacacctg accagattgt aaagctcgcg tcgggccgcg
     1201 tgctcgtggt tggcgacgtc atggtcgacc actatgtgac cggccgcgtc tcgcgcgtat
     1261 cagatgaagc gccggttccg atcatccatg tctcaggcga acgctggacg ccaggcggcg
     1321 cagccaacgt tgcggcgaac atcgcctcgc tgggcggcgg cgccgtgctt gtcggcgtcg
     1381 tcggcaagga cgcagccgca acaaccttgt ctgacctgct ggcgccgctc gccgaatctg
     1441 ctcgtgcgga cctcgtcgtg gaggctggac ggccgacgac gatcaagaca cgctatatgg
     1501 gcggccaaca ccagatcgtg cgtgtcgatc gcgaagagcc ggcgccgatc tcgagcgaga
     1561 cggaagccgc cctcgtcgcc gctatcgaaa aacatgctcg cgactgccag gcggtgacga
     1621 tctcggacta cgccaagggt gcgctgaccg atcgtgtcat caaggcggct atccacgccg
     1681 cacgcagcgc gggcgttccg gttgtcgtcg atcccaagcg tgcggactgg acagcgtatg
     1741 gcggcgcgac ggtaatcacg cccaaccgca aggagctgca gctcgccagc cacgtgccct
     1801 gctccagtga cgatgactgc gcccgcgccg ccggctttgc catcaaggcg acgcgcgccg
     1861 ccatcctgct cacacgctcc gaacacggca tgtcgctcta tcgcgatggc aagccaccgg
     1921 tgagtctcgc cgccaaggcg cgcgaagtgt tcgacgtctc cggcgccggc gacaccgtgg
     1981 ctgcctccgt cgcacttggg cttgcctcag ggctcgaact tgaagccgcg atgcaggtcg
     2041 cgaacaccgc agcaggcatt gtcgtcggca agcgtggcac agcgacggtc tcccccgctg
     2101 aactgctggc cgaaaccggc ggcggtcgcg ggctgggcgg ctcgccgggc gccgttcccc
     2161 tggcggaagc gaggaagctt cgcgaaaaat ggggccgcga cggcctcgca gtgggcttca
     2221 caaacggctg cttcgacatc ctccatgccg gccacattgc actgttgaaa caggccgcgg
     2281 gcgcctgcga ccggcttgtt gtggcgctga ataccgacgc gtccgtgcgc ggcctcaagg
     2341 ggccggggcg gccggtccag tcagaacagg ttcgcgcagc cgtgatggcc gcaatcaagg
     2401 gcgtcgacgt cgtagtcctg ttcggcgagg aaacgccgct cgaagtcatt cgcgagctgc
     2461 agccggacgt gctgatcaag ggcgcggact atgccgaaga ccagatcatc ggcgccgaca
     2521 tcgtccgcgc gcgtggcggc cggattgtcc gggtcaccct ggtcgacgga caatcgacca
     2581 gcagcgtcat cgaaagatcc aaacgaggcg cctagcagga taccaagcct cggcgcttag
     2641 cggcggatta cgaagaaggc gagctgtgtg ttctggtcac gatgcttgcc gcccacgccg
     2701 gccgcgtaga aagcagcagc cgctccggca agaagcgagg ccgcagtgat gaaggcagac
     2761 agtatagtca gcttcctcgc tgcttcggcc ctgcgttccg aaagcgtttc atctgccgcc
     2821 tcgcggttca ccgcaggctc ccgagccgcc gcttcgtcga cttcctggct ggccgctctc
     2881 gcaacagagc tgacgatatc gccgcc
//
LOCUS       DASUGB010001101         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_678442, whole genome shotgun sequence.
ACCESSION   DASUGB010001101 DASUGB010000000
VERSION     DASUGB010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678442"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFV87_20890"
     CDS             <1..879
                     /locus_tag="VFV87_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106295.1"
                     /translation="CGEFSIPWSLHMASRFVCVGILLVAALLGADSLPAQEQPPGEDA
                     QRNKRLTLMENSIAALEARSDEIRDKSVLTFTAKPLLRYNDPTREYVANLLLDATVWR
                     LGESGRPTALVTLEMYSGSGKEGLLSFEFASLTNAGFSLRHKANENVAWRATGSAVKM
                     SPLAEAPPPAKSAAGRLAQMRRLARDFKVHERLGDGQTVECRLLAQPINRYTSAADKI
                     SDGAIFAFANGTNPELGLLLECDDQRWSYGAVRLSSAELTLALGDRELVKFQPGDFRA
                     TSGTYSAAHHPLKLAD"
     gene            complement(1013..1795)
                     /locus_tag="VFV87_20895"
     CDS             complement(1013..1795)
                     /locus_tag="VFV87_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106296.1"
                     /translation="MRALIFCILFVACSFGRQTARAEENSLADAHRAFDQYLHEIDQI
                     ERQAAQSKAEARARLLDALGLKGAFPPAQAQKATPGLIGTAIVDGRPSGVAFHYEHGK
                     LFPRELVWDRFHKQVDGQTYFPDLNITLVGHVEVPHEMTAKVSQAAGGVNSDHGTLFI
                     DDRQIGQVGDDTAKAEIYLLTLSAGIHSVRWVLTAGTFQNSQLKFEDPRTGELLKVFY
                     DEVQRRETGAADAREWVEAAADPSEWVKAMGPNHWRWVPLAK"
     gene            complement(1947..2315)
                     /locus_tag="VFV87_20900"
     CDS             complement(1947..2315)
                     /locus_tag="VFV87_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106297.1"
                     /translation="MRKLIVAGVVLAFAWVAVSLVGAEEEKEKASIKEVMKVCMKGGL
                     CGKVAKGEASEEEKKKLAGLFAALHEAKPPKGSAESWDLKTKALVEAANDVLAGKEGA
                     ADKLKAAANCMACHKEHKGA"
     gene            complement(2467..2997)
                     /locus_tag="VFV87_20905"
     CDS             complement(2467..2997)
                     /locus_tag="VFV87_20905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5106298.1"
                     /translation="MPGATPGRNPACCLARSSAPVRPRLRARRGAFTLVEVMMVLAAL
                     AIIAGVVIPQVTGIIDDAKNSAMLRDLRELAVAIERYRMEHNGAAPDLIQNDTLIQLV
                     SKTDMDGNLGSGPECVYGPYMEKLPPNALNNVGRVFRVNTAPPPNLTSRVGWVYHEET
                     GQIWAGLHNGPVVLGP"
     gene            3141..>3801
                     /locus_tag="VFV87_20910"
     CDS             3141..>3801
                     /locus_tag="VFV87_20910"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106299.1"
                     /translation="MLATLTAIDYGVVALFLAALVAAVFYFSSRQKDTGEFFLAGRSL
                     DWLPLGMSLAAILVSLLGFSFLPGQAYETGWKCWIVVLPFWLALPLIAYVAIPLFRGL
                     GVVSLYEYLELRFDARVRLVASVIFVAWRLVWLAGAIYLPCLAIRLAAGWSVPVWLIV
                     IPVGGLATLGAFLGGMREVTWSGLLKFGAVVAGTVTIIAGILLAMEDGPSRVAEVTRG
                     LG"
BASE COUNT          628 a         1202 c         1190 g          781 t
ORIGIN      
        1 tgcggcgagt tctcgatccc gtggagtctg cacatggcca gtcgattcgt ttgcgtcggc
       61 attctccttg tcgcggcact cctcggcgcc gattccctgc cggcccagga acagcctccc
      121 ggcgaagatg cccagcgaaa caagcggctg acgctgatgg agaattcgat cgcggctctt
      181 gaggcgcggt cggatgagat tcgcgacaag tccgtcctga ccttcactgc gaagccgctc
      241 ttgcgttaca acgatcctac gcgcgagtac gtcgcgaacc tgctgctgga tgccacggtc
      301 tggcgcctcg gagaatccgg ccgaccgacg gcgctggtaa cgttggaaat gtactccgga
      361 tccggcaaag aaggtttgct gtcgttcgaa tttgcgtcgc tgaccaacgc cgggttctcg
      421 ctgcggcaca aggccaacga gaatgtcgcg tggcgggcga cggggtcggc cgtaaagatg
      481 tcgccgctgg cggaggcgcc tccgccggcg aaatccgccg ccggccggct ggcgcagatg
      541 cggcggctcg cgcgcgactt caaggtccat gagcggctcg gcgacgggca aacggtcgaa
      601 tgccgcctcc tcgctcagcc gatcaatcgc tatacctcgg ccgcggacaa gatctcggac
      661 ggggccatct tcgcgttcgc caatggaacg aaccccgagc tcggcttgct cctggagtgc
      721 gatgaccagc gctggtcgta cggcgcggtg cggctgagct cggccgagct taccttggcg
      781 ctgggcgatc gtgagctggt caaatttcag cccggcgact ttcgcgcgac aagcggcacc
      841 tattccgcgg cacatcatcc gctgaagctg gcggattaga taggctttgt ttgaaaacgg
      901 atgaagacgt gggggtggct ggggttgagc gatagctcta ccccagcgat ccgggttatt
      961 gcttcgcaaa aacccggcca ccccccgcta cgcattgacg aggtcctagc agctatttgg
     1021 ccagcggcac ccaccgccag tgattcggtc ccatcgcttt gacccactcg ctcggatcgg
     1081 cagcggcctc aacccattcg cgggcatcgg ccgcgccggt ttcgcggcgt tggacttcat
     1141 cgtagaagac tttcagcaat tcgccggtgc gcggatcctc gaacttgagc tggctgttct
     1201 gaaacgtgcc agccgtcagc acccagcgca ccgagtgaat gcccgcgctg agcgtcagca
     1261 gatagatctc cgccttcgcc gtgtcgtcgc ccacctggcc gatttggcgg tcatcgatga
     1321 agagcgtgcc atggtcgctg ttcaccccgc ccgcggcttg cgacaccttg gcggtcatct
     1381 cgtgcggcac ttcgacgtgg ccgaccagcg taatgttcaa atccggaaag taagtctggc
     1441 catccacttg cttgtggaac cggtcccaga ccagttcacg cgggaacagc ttgccgtgct
     1501 cgtaatgaaa cgcaacgccc gacggccggc catcgacgat tgcggtcccg atcagccccg
     1561 gtgtggcctt ctgagcttgg gcgggcggga aggcgccctt cagaccgagt gcgtccaaga
     1621 gcctcgcccg ggcctccgcc ttcgactggg ccgcctgcct ctcgatttgg tcgatttcgt
     1681 gcaggtattg atcgaatgcc ctatgagcgt cagcaagcga gttttcctca gcccgcgcag
     1741 tttgacggcc gaatgagcag gcgacgaaca ggatgcagaa aatgagcgcg cgcatgggct
     1801 ttctcctcga aggatgacca gtaccggcct tcagaagatg cgacgaggct gccctcgcgt
     1861 tggatccggc gcggcccgga aacgacgcaa cccgccggtt gaatccagcg ggctgcgaga
     1921 tattgacgat ttctgaatcc agagcgttag gcgcccttgt gctccttgtg gcacgccatg
     1981 cagttggcgg ctgccttcag cttatcggct gcgccttcct tgcctgccaa aacgtcgtta
     2041 gccgcttcga cgagggcctt ggtcttcaaa tcccagcttt cagcgctccc cttcgggggc
     2101 ttcgcttcgt gcagggcggc gaacaggcca gccagcttct ttttctcttc ctcgctggcc
     2161 tcacccttgg ctaccttgcc gcaaagaccg cccttcatgc agaccttcat cacctccttg
     2221 atggaagcct tctctttctc ttcctcggcc ccgaccaggc tgaccgccac ccaggcgaag
     2281 gcgagcacga ctccagccac aatcaacttg cgcataccag gatctccgct catcaaagta
     2341 agttgggaaa aatggaactg accgtgcggg gtcatcgtac acgaggggaa accaaaaatc
     2401 tgctacggcc ctggcacttt gcgcgaaatt caccccgatt ccccgctaga gggggtaact
     2461 agaggctcac ggccccaaga ccaccggccc gttgtgcaag ccggcccaga tctgtccggt
     2521 ttcctcgtga taaacccagc ccacgcggct ggtcaggttc ggggggggcg ccgtattgac
     2581 gcgaaatacg cggcccacgt tgttgagggc gttgggcggc agcttctcca tataagggcc
     2641 atacacgcat tccggcccgc tgcccaggtt gccgtccatg tctgtcttgc tcacaagctg
     2701 gatcagcgtg tcgttctgaa tcaggtcggg cgcggcgccg ttatgttcca tgcgataacg
     2761 ctcgatcgcc acggccagct cgcgcaggtc gcggagcatc gcgctgttct tcgcgtcgtc
     2821 gataattccc gttacctgcg ggattacaac gcccgcaatg atcgccagag ccgccagcac
     2881 catcatgact tcgacgagcg tgaacgcgcc gcggcgggcg cgcagccgcg gacgaacggg
     2941 cgcgctgctg cgcgccagac agcaggcagg attgcggcct ggagttgcgc ctggcatggt
     3001 ggagagggct tacgctttgg cagcgcaaga ataatccttg ggaaatcgta ggtcacgtgg
     3061 cgtcacacgg ctgccagcgg cggcaatagt tcttacgatg ccggttgttc cgattttgcc
     3121 attcgccttg cagcctgctc atgctcgcca cgttgaccgc catcgactat ggggttgtgg
     3181 ctttattcct ggccgcactg gtggcggcgg tcttctactt cagctcccgg cagaaggaca
     3241 cgggcgagtt cttcctcgcc gggcgctcgc tcgattggct gccgctgggg atgtcgctgg
     3301 cggcgattct cgtgtcgctc ctgggcttca gctttctccc gggccaggcc tacgagaccg
     3361 gttggaagtg ctggatcgtc gtgctgccct tttggctggc cttgccgctg atcgcgtacg
     3421 tcgcgattcc gctgttccgc ggcttgggcg tcgtttcgct ctacgaatac ctggaactcc
     3481 gctttgacgc ccgcgtgcgg ctggtggcga gcgtcatctt cgtcgcctgg cggctggtct
     3541 ggctggcggg cgccatttat ctcccctgcc tggcgattcg cctggctgcc ggctggagcg
     3601 tgccggtgtg gctgattgtg attccggtgg ggggcctggc gacgctgggt gcattcctgg
     3661 gcggcatgcg agaagtaacc tggagcgggc tgcttaaatt cggcgcggtc gtggccggaa
     3721 ctgtgacgat catcgccggc attctgctgg caatggagga cggcccgtcc cgcgttgccg
     3781 aggtgacacg ggggttaggc c
//
LOCUS       DASUGB010001102         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_262987, whole genome shotgun sequence.
ACCESSION   DASUGB010001102 DASUGB010000000
VERSION     DASUGB010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262987"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..947
                     /locus_tag="VFV87_20915"
     CDS             198..947
                     /locus_tag="VFV87_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106300.1"
                     /translation="MALGSPSPLLTVPTDRQERWKLVREVIRQWFPADLSKLALPLGM
                     SPITAPPTACGAIQEWYRIVREAPEVWCQQDHLFGAGGDWRRGDYLTIAVENQVCAYW
                     GVPRSELDQEDPPVFLDRGRGAWEVESPTTSEFALTWLAYSIKWSHYNRGGANGSLDD
                     TALELIKERFPRLKLTDWHWPDGPTRFYGTADLLLETNGDNPGAWLWVSARTKAAFRE
                     FQSLVSTTEIRWESSSDEWPDGWVSAADDPE"
     gene            1368..1904
                     /locus_tag="VFV87_20920"
     CDS             1368..1904
                     /locus_tag="VFV87_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HEX5106301.1"
                     /translation="MAKRDNHYEAALEAYLRWLAIPYVAVDETRRTLAGDQSLKSLDF
                     IVSPPAAAGSLLIDVKGRRFPTAGKQYWRNWTTRSELASLAAWEELLGPAATGHLVFA
                     YNVVGDRAPLPPEELFVFRGSLYGFIAIRLDHYSTFATQLSARWDTVSIPTAQFRALA
                     RPLRELCCVGLPQGAGAA"
     gene            complement(1985..>3499)
                     /locus_tag="VFV87_20925"
     CDS             complement(1985..>3499)
                     /locus_tag="VFV87_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5106302.1"
                     /translation="EVVGCTREDCAGFAADAYARITGLGAVCTTYCVGGLSVCNSIAG
                     AYAEKSPVVVISGAPGMRERMNNPLLHHKVKDFRTQIEVFEKLCAACTELTDPAVAFR
                     EIDRVLDTVVRYKRPGYIEIPRDLVEVVPQIVHTYHHGETPADPQVADEAADETAELL
                     AKAQRPVILAGVEIHRFGLQDKVLALAEQYKIPMAATLLGKSVVPETHPLYIGLYEGA
                     MGRVEVTQYVEESDCVLLLGAFMTDINLGIFTAQLDPARCIYVTSEQLRIRHHHFHHV
                     PLDLFLDKLLARELQVTPRPIPPELRPPRSPFKLEPGAPLTIRRMIALLDEQIDGETI
                     VIADIGDALFAATELTTHQRTEFISPAYYTSMGFGTPAALGALMARPQKRVLTICGDG
                     AFQMTGMELSTIVRRGLAPIVIVLNNCGYGTERYLHPGQWQYNDIHCWQYHKLPEVLG
                     GGTGYEVGTEGEFDAAIGRAWNDRRAVSLINAHIPIGDASDALKRLTERLSKHV"
BASE COUNT          601 a         1131 c         1079 g          688 t
ORIGIN      
        1 ttccatcagt tggctccaga atttccggcg actggtcacg cggtacgaat ttcacgctca
       61 cctgtttcat ggcttcgtac aactggcctg cttgatcgtc gtcctaggac ggttttgaaa
      121 tgcgctctag attcttagga agacgattaa gataaggagt ccgattaaga ccaggattct
      181 caatggagcg tggaccgatg gcgctcggct ccccttcgcc gctgctgacg gtgccgaccg
      241 accggcaaga gcgctggaag ctggtgcgcg aggtgatccg gcaatggttt ccggctgacc
      301 tcagcaagct cgccctgcca cttgggatgt cgccaatcac cgcgccccct acggcgtgtg
      361 gtgccattca ggagtggtac aggatcgtcc gcgaagcgcc cgaggtctgg tgccagcaag
      421 atcacctgtt cggtgcgggt ggagactggc ggcgcggcga ctacctgacg atcgccgtcg
      481 agaatcaggt gtgcgcatac tggggagttc cgcggagtga gctggatcag gaagatccgc
      541 ccgtgtttct cgatcgtggc cgtggagcgt gggaagtcga aagcccgacc accagcgagt
      601 tcgcgctcac ctggctggcc tacagcatca aatggtcgca ctacaaccgc ggcggggcca
      661 acggatcgct ggacgacacc gcactggaac tcatcaagga gcggtttccc cggttgaagt
      721 tgactgactg gcactggccc gacgggccga ctcggtttta tggcaccgcc gacttgctgc
      781 tcgaaaccaa tggcgacaat cccggcgcgt ggttgtgggt ttctgcccgc acgaaggctg
      841 cgtttcgaga gtttcagagc ctcgtctcga ccacggaaat tcgctgggag tcctcctccg
      901 acgaatggcc cgacggctgg gtgagtgctg ccgacgatcc ggagtagtcc tcccatcgtt
      961 gtctcctagc ggtgcaggcg agtacgggtt cctctgctct gatccggaaa gagagaagag
     1021 gaacccgtac ttccgcgccg tttggtgcgg ggcggaattg aaagacaaac aggtcacaaa
     1081 ggaggagcct catcttccct cttcgtgatc ttggcgcctt tgtggttgaa atcctccaag
     1141 gcgggctagt ttgcaaccat ccaaccatgg aagactcaat acacaaattg aactcgagcg
     1201 gcgcccagcc tttccgtgtg tttcgtgttt tccgtggttc tctccctcct tcaatcgtcc
     1261 gattcgtctg attcgtggtt tcccgtgtcc gccaggtccg gcttggcagg tccagcgccg
     1321 gtcgcttacg atttgtgctg gcggaagcgc gtcaggaggc ttgcggcatg gccaagcgcg
     1381 acaaccatta cgaggcggcg ctggaagcgt accttcgctg gctggcgata ccgtacgtcg
     1441 ccgtcgatga aacccgccgc acgctggccg gcgaccagtc gctcaagagc ctcgatttca
     1501 ttgtctcgcc gcccgccgct gcgggcagct tgctgatcga cgtcaagggc cgccgcttcc
     1561 cgacggcggg caagcagtac tggcggaact ggacgacccg cagcgagctg gccagcctcg
     1621 ccgcctggga ggaactgctc ggccccgccg ccaccggcca cctcgtcttc gcctacaacg
     1681 tcgtgggcga ccgcgcgccc ttgccgcccg aagagctgtt cgtcttccgc ggcagcctgt
     1741 acggcttcat cgcgatccgt ctcgaccact actccacgtt cgccacgcag ctctccgcgc
     1801 ggtgggatac cgtcagcatc cccaccgccc agttccgcgc cctggcccgg cctttgcgcg
     1861 agctgtgctg cgtgggcctg ccgcaagggg cgggagcggc gtaatccatt ttggattttc
     1921 gattttggat tttggattgg atgcagttga atccaaaatc aaaaatccaa aatccaaaat
     1981 tgaatcacac gtgcttgctc aaccgctcgg tcagccgctt cagggcgtcg ctggcgtcgc
     2041 cgatcgggat gtgggcattg atcaggctca cggcccgccg gtcgttccag gcccggccaa
     2101 tcgccgcgtc gaattctcct tcggtcccca cctcgtagcc cgtgccgccg ccgagcactt
     2161 cgggcagctt gtggtattgc cagcaatgga tgtcgttgta ttgccactgg cccgggtgca
     2221 ggtaccgctc ggtgccgtag ccgcagttgt tgagcacgat cacgatcggc gccagccccc
     2281 gccgcacgat cgtcgaaagc tccatcccgg tcatctgaaa cgctccgtcg ccgcagatcg
     2341 tcagcacccg cttttgcggc cgggccatca gcgcccccag cgccgccggc gtgccgaagc
     2401 ccatcgacgt gtagtaagcc gggctgatga attccgtccg ctggtgggtc gtcagctcgg
     2461 tggcggcaaa cagggcgtcg ccgatgtcgg caatcacgat cgtctcgccg tcgatctgct
     2521 cgtccaagag cgcgatcatg cggcgaatcg tcagcggcgc gccgggctcg agcttgaacg
     2581 gcgagcgcgg cggacgcagc tccggcggga tcggccgggg ggtcacttgc agctcgcggg
     2641 ccagcagctt gtcgaggaac aagtccagcg gcacatgatg gaaatggtgg tgccgaatgc
     2701 ggagctgttc gctcgtcaca taaatgcacc tggccggatc gagctgagcc gtaaagatcc
     2761 cgaggttgat gtcggtcatg aacgccccga gcagcaggac gcagtcgctt tcctccacgt
     2821 actgcgtcac ctcgacccgc cccatcgccc cttcatacag gccgatataa agcggatgcg
     2881 tttcggggac cacgctctta cccagcagcg tcgccgccat cggaatttta tattgctcgg
     2941 cgagggccag caccttatcc tgcaagccga agcggtggat ttcgacgccc gccagaatca
     3001 ccggccgctg ggccttggcc agcagctccg ccgtctcgtc ggccgcttcg tcggccacct
     3061 gcgggtcggc gggcgtctcg ccgtggtgat aagtgtgtac gatctgcggc accacctcga
     3121 ccaggtcccg tgggatttcg atataccccg gccgcttgta gcgcacgacc gtatcgagca
     3181 cgcggtcgat ctcgcggaag gccacggccg ggtcggtcag ctcggtgcag gcggcgcaca
     3241 gcttctcgaa cacttcgatc tgcgtgcgga agtccttcac cttgtggtgc aagagcggat
     3301 tgttcatccg ctcgcgcatc cccggcgcgc cgctgatcac cacgaccggc gacttttcgg
     3361 cgtaggcccc ggcaatcgag ttgcagaccg acaggccgcc cacgcagtac gtcgtgcaca
     3421 ccgcgcccag gcccgtgatc cgggcatagg cgtcggctgc gaaccccgcg cagtcttcgc
     3481 gcgtgcaacc cacgacctc
//
LOCUS       DASUGB010001103         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1373598, whole genome shotgun sequence.
ACCESSION   DASUGB010001103 DASUGB010000000
VERSION     DASUGB010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373598"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFV87_20930"
     CDS             <1..934
                     /locus_tag="VFV87_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5106303.1"
                     /translation="SGMAATHCAMMLAQSGQHVLAGSDIYGGTFRLLHKVLNQAGVTV
                     SLADSTDPAAFEAAITPETRLLWIESPGNPRMTITDIAAIAIIAKRRGVLLGVDNTFA
                     TPVLTRPLELGADIVMHSATKYLGGHSDLLGGALVVKDKALLDKLYFLQNATGGVMGP
                     FESFLASRGLKTLELRVREQSRTAQQLAEWLSQHPKVSRVLYPGLPAHQGHEIARRQM
                     DGAFGAMLTFEARGDFRAAKRICETTQLFQLAVSLGAVESLIEQPASMSHASYDPAAR
                     AAHGITDGLIRLSVGLEAFEDLQRDLDQALQSSG"
     gene            complement(1036..1812)
                     /locus_tag="VFV87_20935"
     CDS             complement(1036..1812)
                     /locus_tag="VFV87_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106304.1"
                     /translation="MGFRAALLAVVVAGSPAVATAQYQQYATPRSPSVVQPAGVWHNY
                     AVVPDGHCGCAMPVRCDNYTPCCSVCNFHPVCLLKNVGRMLDCLLPCGRCCHGGCGIG
                     CGVPCCRGGYGGGYPMGCPSCSTPALSDPFRDDPEPLTPPAPVPAAEVRRHSSSDSPY
                     NVIRDPRPDAQASTDFAPERGQSQPRGTSRRSPIVTTKPAARKPSEQSILRRASLEEE
                     TTQIAEPATLEVEAAEARPIQRAAASSDDYWSNVPRNPLR"
BASE COUNT          364 a          696 c          656 g          353 t
ORIGIN      
        1 cagcggcatg gcggcgacgc actgcgcgat gatgctcgcc cagagcggtc agcacgtgct
       61 ggccggcagt gacatttatg gcggcacgtt tcgcctcttg cacaaggtgc tcaatcaggc
      121 gggcgtgacc gtctcgctgg ccgatagcac cgaccccgcg gccttcgagg cggcgatcac
      181 gccagagacc aggctcttgt ggatcgagag tcccggcaat ccgcggatga cgatcaccga
      241 tatcgcggct atcgccatta tcgccaagcg ccgcggcgtt ctgctcggcg tcgataacac
      301 cttcgccacg ccagtgctca ctcgtccgct ggagctgggt gccgatatcg tgatgcactc
      361 ggccaccaag tatctcggcg ggcatagcga cctgctcggc ggggcgctcg tggtcaagga
      421 caaggcgctt ctcgacaagc tctatttcct gcagaacgcg accggcggcg tgatgggccc
      481 cttcgaatcg ttcctcgcca gccgcggcct gaaaacgctc gagcttcgcg tccgcgagca
      541 gtcgcgcacg gcccagcagt tggcggagtg gctctcgcag catccgaagg tcagccgcgt
      601 gctctatccg ggtctgccgg cacatcaggg ccacgaaatc gcccgccggc agatggacgg
      661 cgcgttcggc gcgatgctga cattcgaggc ccgcggcgat ttcagggcgg ccaagcgcat
      721 ctgcgaaacg acgcaactct tccagctcgc cgtcagcttg ggcgcggtcg aatcgctcat
      781 cgagcagccg gcgtcgatgt cgcacgccag ctacgacccg gccgcacgag ccgctcacgg
      841 cattactgac gggttgatcc gcctctcggt tggcctggaa gcgttcgagg acctgcagcg
      901 cgacctcgat caggcgctcc agagctccgg ctgatcgccc ggcaacaaaa aaaccagccg
      961 gcccgtcgga cgcggatcgg ctggcgaaag gcaagaagtc gctcagacaa cttgtacggt
     1021 ttggcgtgtg atcgactaac gcagcgggtt ccgcgggacg ttcgaccagt agtcgtcgct
     1081 gctcgcggcc gcccgctgaa tcggcctggc ttcggccgct tccacttcca gtgtcgccgg
     1141 ctcggcgatt tgcgtggtct cttcttcgag cgaggcgcgg cgcagaatcg actgctcgct
     1201 gggtttgcga gcggccggtt tggtggtcac tatcggcgag cgacgcgacg tgcctcgcgg
     1261 ctgcgattgg cctcgttccg gtgcaaagtc ggtgctcgcc tgagcgtccg gtcgcggatc
     1321 acgaatgacg ttgtagggcg aatccgacga cgaatgccgg cgcacttcgg cggccggcac
     1381 gggggccggc ggagtcagcg gctccggatc gtcgcggaac ggatcggaga gcgcgggcgt
     1441 gctgcacgag gggcaaccca tcggatagcc ccccccatat ccgccgcggc aacagggcac
     1501 gccgcatcca atgccgcatc caccgtggca gcacctgccg cacggcagca ggcagtcgag
     1561 catgcggccc acattcttca gcaggcagac ggggtggaag ttgcacaccg aacagcacgg
     1621 tgtgtagttg tcacaccgca ccggcatcgc acatccgcag tgcccgtcgg gcaccacggc
     1681 ataattgtgc cagacacctg ccggctgcac gaccgacggc gaacgcggcg tcgcgtactg
     1741 ctgatattga gccgttgcca ccgcgggcga ccccgccacg accaccgcca acaatgctgc
     1801 gcggaaaccc atgctccccc ctccttggtg tggatgccct gcccatgatg gcgcactcag
     1861 gccgtttggc cctcagaggc gtcatggaaa gagtcttccc tatctccaac acagttgatc
     1921 gaaccgccac aagcggattg gcgagaactt tcgccgcagg cggcaaagtc gtagcaatca
     1981 ctgcagttgt cctatcttca ctgtcttttc aaaacacgcg ccttcaatca gcccgtcgtc
     2041 cacgccgcgc tggacgacgc ggctaagca
//
LOCUS       DASUGB010001104         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_540921, whole genome shotgun sequence.
ACCESSION   DASUGB010001104 DASUGB010000000
VERSION     DASUGB010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540921"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /locus_tag="VFV87_20940"
     CDS             <1..1837
                     /locus_tag="VFV87_20940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX5106305.1"
                     /translation="RVAAWKDWEVAPASGGTLAEAGLEISLVRDERRPPPGEILAAVQ
                     VKNRPLVLRRELAIGPHDNWLVIAATRPFSRGQEPKVEVKIGGEPVAEFAVPEWQTDP
                     NDSRPLAVSLAGYRQAAAERTVNVEVIQQSAVDSAPVLYRSITTIDELPTLHRVLEEQ
                     ALPVPIAANQAGGASLTSEDRYYGKNSLKVSPGGQFRLELARTIAIRERPKWGEARFI
                     RFAVRKQSGGRFALELAGPQPRDPPARYDLGRGEPSYGSATRIWQDNLPKDWVAITRD
                     LFADFGSLDVNAVVVGSPDNGDALVDHIYLARGPQDFDLIPAAPSVELVNDKARRELA
                     APLIEKNSPATVRIEFTDGRQAAGVLISPQGEILTAGHAVIGANRDCRVTLSDGTTAA
                     ARTLGVARDFDLGLVRITPDGNYPIIPPHTPADLPQNQVYLAFTYPPRVQEFRRGDPY
                     LVQIRRLFRSTVWIDLTADDWIAGGPLIDRDGRLVAIQVAKSQFGGVLCTRFQEPWQH
                     LPRLRNGEVFGAWPPGSEPATGFAVAAAEGAVTVKSVAAASSAEKAGLQVGDLIQKVD
                     AQPTASEADVQRAIAAHDAGQEVALDLTRGGMALQVRFVLTPRVP"
     gene            complement(1878..2573)
                     /locus_tag="VFV87_20945"
     CDS             complement(1878..2573)
                     /locus_tag="VFV87_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5106306.1"
                     /translation="MKMTICRSVFALVLVGFAGLAVGADQDGVKSLFDGQTLEGWSGK
                     EQFWSVKDGAITGQTTPDNPTKGNTFLIWKGGDVKDFELRLKFKIVGGNSGIQYRSTD
                     MGDFVVGGYQGDIEAGDKYIGILYEERGRGILAERGAKVVIHPDGKKEVVGKTGDSKE
                     IAASVKKEDWNEYEVIAKGNHLIQKINGYVTVDVTDNQSDKAKESGILALQLHAGPPM
                     TVQFKDIQYKELK"
     gene            complement(2725..2958)
                     /locus_tag="VFV87_20950"
     CDS             complement(2725..2958)
                     /locus_tag="VFV87_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106307.1"
                     /translation="MGAAAGWQHEDAASAWQQSLFAERSPAGVLPVQQQPGEFSAAAA
                     QAQLKHPQTASRSTGAMAKATAASRAAQFAGAF"
BASE COUNT          626 a         1253 c         1182 g          753 t
ORIGIN      
        1 gcgcgtggcc gcctggaaag attgggaggt cgcgcccgcg agcggcggca cgctggccga
       61 agcggggctg gagatttcgc tggttcgcga cgagcgtcgc ccgccgccgg gtgaaatcct
      121 ggccgcagtg caagtgaaaa atcgcccgct ggtactgcgg cgcgagctgg cgatcggccc
      181 ccacgataac tggctggtca tcgccgccac gcgccccttc agccgcggtc aggagccgaa
      241 ggtggaagtc aaaatcggcg gcgagcccgt ggccgagttc gccgtccccg agtggcaaac
      301 cgaccccaat gacagccggc cgctggccgt gtcgcttgcc ggttatcggc aggcggcggc
      361 ggagcggaca gtcaacgtcg aagtcatcca gcagtcagcc gttgacagcg ctcccgtgct
      421 ctaccggtcg atcacgacga tcgacgaatt gcccacgctg caccgcgtgc tcgaagagca
      481 ggcgctgccg gtgccgatcg cggcgaatca agctggcggc gcgtcgctga cgagcgagga
      541 tcgctattac ggcaagaact cgctgaaggt ctcgccgggc gggcaattcc ggctcgaact
      601 ggcccggacg atcgccatcc gtgagcgacc gaaatggggc gaggcgcgat tcatccgctt
      661 cgcggtccgc aagcaatcgg ggggccgatt tgcgctcgaa ttggccggcc cgcagccgcg
      721 cgatccgccg gctcggtacg acctgggacg cggcgagccg tcgtatggct cagcgacgcg
      781 gatctggcag gacaacctgc cgaaagactg ggtcgcgatc acgcgcgacc tcttcgcgga
      841 tttcggcagc ctcgatgtga acgccgtcgt ggtcggctcg cccgacaatg gcgacgcgct
      901 cgtggatcat atctatctcg cccgtggtcc gcaggatttc gacttgatcc ccgcggcccc
      961 ctcggtcgag ctggtcaacg acaaggcccg ccgcgagctg gccgcgccgc tgattgaaaa
     1021 gaactcgccg gccaccgtgc ggattgaatt taccgacggc aggcaggcgg caggcgtttt
     1081 gatttcgccg cagggggaaa ttctgaccgc cggccacgcc gtgatcggtg ccaatcgcga
     1141 ctgccgcgtg acccttagcg acggcacgac ggcagcggcc cgcacgctgg gagtggctcg
     1201 tgattttgat ctcggcctgg ttcgcatcac gccggacggc aattatccga tcatcccgcc
     1261 gcacacaccg gcggacctcc cccagaacca ggtctatctg gccttcacct acccgccgcg
     1321 cgtccaggaa ttccgccgag gtgatccgta tctggtgcag atacgccgcc tgtttcgcag
     1381 caccgtctgg atcgacttga ccgctgacga ctggatcgcc ggcgggccgc tgatcgaccg
     1441 cgacgggcgg ctggtggcga ttcaggtcgc caaaagccaa tttggcggcg ttctttgcac
     1501 gcgctttcaa gagccctggc agcacctgcc acgattacgc aacggcgagg tcttcggcgc
     1561 ctggccaccc ggcagcgagc ccgcgaccgg atttgctgtt gccgccgcgg agggtgccgt
     1621 gaccgtcaaa tcggtcgcgg ccgcaagttc tgccgaaaag gccggcctgc aagtcgggga
     1681 tttgattcag aaggtcgacg cccaaccgac cgcctccgaa gccgacgtgc agcgagccat
     1741 cgccgcccac gacgccggcc aggaagtcgc gctcgacctc acacgcggtg gtatggctct
     1801 gcaggttcgc ttcgtgctga cgccgcgcgt gccgtaaagc cggctgccct tgtggttgta
     1861 tccgacgagc cgattggcta cttcagctct ttgtactgaa tgtccttgaa ctgcacggtc
     1921 attggcgggc cggcgtggag ctgcagcgcg aggatgcccg attccttagc cttatcgctc
     1981 tggttgtccg tgacatcgac ggtcacatag ccgttgatct tctggatcag gtgattcccc
     2041 ttggcaatga cctcgtactc gttccagtct tcctttttca cggaagcggc aatttccttg
     2101 ctgtcccccg tcttgcccac gacttccttc ttgccgtcgg gatggatgac gactttggcg
     2161 ccgcgctcag ccagaattcc gcgtccgcgc tcttcataaa gaatgccgat gtacttgtcg
     2221 cccgcctcaa tatcgccctg gtagccgccg acgacgaagt cgcccatgtc ggtgctgcgg
     2281 tattgtatgc cgctgttgcc gccgacgatt ttgaacttga gcctcagttc gaagtccttc
     2341 acgtcccccc ccttccaaat caggaacgtg ttgcccttgg tgggattgtc tggggtcgtc
     2401 tgaccggtga tggccccgtc tttgaccgac cagaactgct ccttgccgct ccagccttcg
     2461 agcgtctggc cgtcgaacag ggatttcacg ccatcctgat ccgcgcccac cgccaaaccg
     2521 gcaaagccga ccagcaccag cgcaaacacc gacctgcaaa tcgtcatttt catgaactga
     2581 gccctcgaat ggaccgtaaa tcctgatgcc tgtaactagt aagcaccagt ggaatccaag
     2641 ccgctggcga ctgcaagtgg ccggtgcgtt gcgcttgact gtgggaaccg acggtcggag
     2701 aatgacgggc atgaggcgac tggctcaaaa cgccccggcg aactgcgcgg ctctgctggc
     2761 agccgtggcc ttagccattg cgcccgtgga tctgctggcg gtctgcgggt gcttgagctg
     2821 cgcttgtgcg gctgccgcgg agaattctcc cggctgctgc tgcacgggca agacgcctgc
     2881 gggcgaccgc tccgcaaaca aagactgctg ccacgccgag gcggcgtcct catgctgcca
     2941 gcctgcagcg gcccccaacc acgacccctg ttcgtgctcg aaagctgtcc cggcggctcc
     3001 cgttggcaca cctcagaaag tcagcgaaaa cgatcaaccg gcgcccccgg catcgcagcc
     3061 tgaattcctg ccgcttgcct ccgattcgct ttccagcgac tctctcgcac cgaaatggga
     3121 ccagcccgtc cggcccgtcc ctccgcgcgt tctcttgtgc gtctggcgga actaatcgtg
     3181 cccggctgcg cgcccattct gctgcgcgcc tgttagcgtt gttctttggc acgaatttca
     3241 ttccgtccga tttcaaggag aaccgtatgt tgtatctcgc tctgttcctg ctctcgctgc
     3301 tggcgccagc gccagccgtc tcgaaggctt cctgctgcaa tggcgcttgt ccggcgagtt
     3361 gcgatccctg ctgctgcgcc acgtgcgatt gcggctgctg cgtcaccggc gtttgcacgg
     3421 gcgattgcgg ctgcacctgc gactgctgcg ccgacggcac gtgtgagtgc gcaaggtagc
     3481 tgatgccctt gcgaaggatg atttggtgga gtactgagaa gtctgagctc agtactccac
     3541 tcgactgagg ccacgacgat caacgattct cgtggagcgc ggacgacgaa gattcgtccg
     3601 ccccttccaa ctttttatcg gggctggacc catctgcggc ttctgccggc ggatctcgac
     3661 tttcatcaac tgtcgtgggc gtcgcgccgg agggagaatc tgcgtcagga cgagtcgctg
     3721 gcagggagga gttatccttc gattctgcag gcggcggttg atctcctggt ggcgtattgc
     3781 ccgccggttc tccctgacct tggtttttgc cctt
//
LOCUS       DASUGB010001105        12958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_817467, whole genome shotgun sequence.
ACCESSION   DASUGB010001105 DASUGB010000000
VERSION     DASUGB010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12958
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817467"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VFV87_20955"
     CDS             complement(<1..998)
                     /locus_tag="VFV87_20955"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5106308.1"
                     /translation="MNACAIILLLAAAPPNPVDFDTEVLPVLAKAGCNAGACHGAAAG
                     RGGFKLSLFGGDPAADYRQIVHELEGRRVNLAHPERSLLLLKPTWQLDHEGGERFAAD
                     SPQAKLLTDWIAAGALRESKKTLVGIRISYSEEPSQALPRHLTLHVSASFSDGSTRPV
                     TESTIFSPADPAATVVDDKGRVQVLSPGRHTIVVRHLTQVAAMQITVPYPGKRLKTTD
                     MPRSNWIDDEINSSLQELRIPPAPRADDATLLRRVTLDLTGRLPAPGRVLEYLSDQDE
                     HKFDAEVERLIASPDFIEYWTYRLAEWLRFENAPREPAAQKAYYDWLKQQATGFHPL"
     gene            complement(1088..2110)
                     /locus_tag="VFV87_20960"
     CDS             complement(1088..2110)
                     /locus_tag="VFV87_20960"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX5106309.1"
                     /translation="MSHPDQPSPDEYVIAPASESDSRAAALLPTEPSEDVSHLLGAID
                     LPSPKPEESERDYLPELAVDWVITAQFAGRPKLAPSHVNALFDRNWRERFGGLTLYGR
                     DAADGRWTYLISSDGPSAVTELKLAWDYASPLEEDEAATTAAQFAARLKEVERRLAAF
                     GKVQVSASQSPEEAAARSETLKEVQREWNYPAVLVLEAPRGQKFSGKQIWDVMLCLGL
                     RWGDMDCFHWRNPSEVGDDHWFSVETSTPPGYFLPEEIAANRVHTTDLVFSLSVPRCA
                     APVEVFEAMIEAAQYARRRLGGTICDGDGNPADLDALCSDIQKIESYLGEIGIPCGSG
                     TALRLF"
     gene            complement(2230..2922)
                     /locus_tag="VFV87_20965"
     CDS             complement(2230..2922)
                     /locus_tag="VFV87_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5106310.1"
                     /translation="MSFLSGWAMSGITIRVLDGADRGRVFQNLHTPITIGREEGNTIQ
                     LNDERVSRYHVKIQEDHDRLVITDLESTNGTKINGEDVQLRILRFGDMIALGRSVLLI
                     GSREQIAQRLARLRAGENDASRTADPEELEKAANISSLDFELNWSEDADLQATIHALE
                     PPELPERLTPGQAAQLAELLEFLHLRLRNLIGSVQVDSKSNKVALDLRQWQALIDLQA
                     RLAEYLRQVGEP"
     gene            complement(3125..5095)
                     /gene="argS"
                     /locus_tag="VFV87_20970"
     CDS             complement(3125..5095)
                     /gene="argS"
                     /locus_tag="VFV87_20970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5106311.1"
                     /translation="MNVLALLKSRFRPVLAELISDPARLEELLDMVRPAQDAKFGDYQ
                     ANFAMTLKKELNRPPPQIAAQVVGATDLADICLPPEVAGPGFINLRLRDDWLTGQLNR
                     AVHDERLGIEPARQPRTYVLDYSSPNVAKPMHVGHIRSTVIGDALCRTLRFLGHRVIS
                     DNHLGDWGTQFGMIIYGYKHFVDRAAFEANPVAELGRLYKFVNRLVDYRASQKALPEA
                     RQRLAQREATAATLQAEPKIGDKAIDKKNAQALAKAQDQVKAAREEVESLQKKIAAVE
                     SDPQLAAAAAKHPNIDQAVLAETAKLHSGDEENLKLWHEFLPNCRDEIARVYGRMNVK
                     FDHEYGESFYHNRLAAVIADLRAKGLARESEGAVCVFLEGFEAPMIVQKSDGAFLYST
                     TDLATIQYRMETWKPDAILYVVDHRQSEHFDKLFAAARLWSHKEVELQHIKFGTVMGA
                     DGKPYKTREGVAAGLESLIDEAVRRAAAVVAENDSDNHLTPEQRQQIAEVVGIGALKD
                     ADLSQNRTSDYTFSYDKMLLMTGNTATYMQYSYARVRSIFAKGGFDPAAVRASGAKIV
                     LSHPAERALGIQLLRFSEALAETEADYMPHHLTGYLFDLAKSYSAFFESCPVLKAETD
                     EIRNSRLLLCDLTARTIRTGLDLLGIQVVEKM"
     gene            complement(5252..5926)
                     /locus_tag="VFV87_20975"
     CDS             complement(5252..5926)
                     /locus_tag="VFV87_20975"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5106312.1"
                     /translation="MRRLISLGLVICTSVALADEGSSGAAGSGQASSAQGSTAARRPL
                     HRHPTLLGMLRRNNDVRRRLGLRPHRLNPALTKAAQDQANYMAQTAQFSHYVNGGPQY
                     RAGKYGFRGGVLENIAYGSRSVDSTFAMWVGSSGHYASIVSNTSDAGFGYAISPSGQC
                     YWVGVYANRASGDAVGETEEEIAKAAQAEEEALAEAETTKDSSVTTASATEGVEDSAP
                     PAESAE"
     gene            6591..8249
                     /locus_tag="VFV87_20980"
     CDS             6591..8249
                     /locus_tag="VFV87_20980"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106313.1"
                     /translation="MRRLTSYFAVALIALAATARAENEGQADLDKATELQIAAETLAD
                     LEKVIELGESALKKGLDEGQTALAKQILAATLYQHADRLSASIFEQSPPSPRWPLVRQ
                     FALRDIEKAKRYDAKLPDLFLLEAKLQVLPGGDEKASHTAIDEAISLLEKGDDKKELA
                     KALILRAAMTDDDERKLADFNSAIKADPENAEAWQGRALLYIEKGESEKAVADLTKIV
                     EKNNSSPLVIGALAEALTNLKKYDEALKYCEDVIKLAPKSTLGYNLRARILIMQDKNK
                     EAIEDLNQALQVNPNDLAALLTRSRLNAAEGKDDLAKADVEKALAIDPELPQGILLRA
                     LLAAQKQKFGEAIADIQVLLQTDPTNPEYRLQLAMFYVGDKRPRRAIDALTSILGGLT
                     DDGDEEQKEIKARALQARGDALLSVGKHAEAVEDYEIALKLEPEDTGVLNNFAWVLAT
                     SPDDNVRNAARSIELGTKACELTKYERPHILSTLAAGYAEKGDWENAVKWSTKASELG
                     AKDPEVGEQLKKELESYQEKKPWREKQEIEENKAPLGKSSDLET"
     gene            8363..8935
                     /locus_tag="VFV87_20985"
     CDS             8363..8935
                     /locus_tag="VFV87_20985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5106314.1"
                     /translation="MDRSSALALMHEHTQSLSLRRHMLAVEAAMRAYAQKYGEDEEKW
                     GIVGLLHDFDYERWPDPPDHPLQGAAILAEQGYPEDVIYAIKSHADYLPDCPRVHLID
                     KALYACDELAGFLTACAMVRPERLEGMQPGSVKKKLKAKGFAASINREDIAKGAADLG
                     VDLDEHIHFVIAAMAAIAEDLGLSVEPSPS"
     gene            complement(8945..10450)
                     /locus_tag="VFV87_20990"
     CDS             complement(8945..10450)
                     /locus_tag="VFV87_20990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5106315.1"
                     /translation="MSLAPPPRPANVRREAPPEILPPAPATSPVALLAEPRAPRVSPR
                     GDMRASIGDAASYSVMVGIGETYFAAFALFLGTGETFAGIIATLPMLVGASLQLATPW
                     ALRRLRSYRAWVVLCASLQAAALFAMPTAALFMGTPAAALWVFVAAAAYWAASQATGP
                     AWNTWIEEIIPRRVRANFFACRARTSQMCTLIGFVAGGIALQLGKAQGWLLAAFAGIF
                     LIGSACRFLSAWFLSTQTEPSCGKYETRSVSLREAIFRTSGDVGVPLVLYLLAVQTAV
                     QISGPYFTPFMLVKQELSYFSYMVLIGIGFLGKVIALPLWGRVAQKAGARRLLWIGGT
                     SIVPVAGLWIFADLFDPWHSQLDLHLGLGVVSVPISGKLIYIGVVQLISGLAWAAYEL
                     AMLLMFFEAIPRQDRASVLTFYNFGNAAALVVGGLIGAAILQLGSESHLAYLVLFGCS
                     SLARLCTVLLLRQAPEPQIEIVQPALRVISVHAEEGGVDRPILPSLDTTGD"
     gene            10746..11462
                     /gene="ubiE"
                     /locus_tag="VFV87_20995"
     CDS             10746..11462
                     /gene="ubiE"
                     /locus_tag="VFV87_20995"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX5106316.1"
                     /translation="MSTTIDKSGERVQRMFGEIAPSYDRMNHLLSLNVDRYWRWWTVN
                     KLQPQPGEPILDVCTGTGDLALAFHRATGGQSEIVGADFTPEMLEIARRKQARAKIGE
                     ELSYLQADTQNLPFDDDRFAIVSVAFGLRNVADTDRGLAEMTRVCRPGGQVAILEFST
                     PRWQPFRGLYLFYFRHILPRVGQLFARNQSAAYEYLPASVGEFLGGEALAERMRGVGL
                     RNVKYWPLTFGIATLYVGKK"
     gene            complement(11516..12106)
                     /locus_tag="VFV87_21000"
     CDS             complement(11516..12106)
                     /locus_tag="VFV87_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106317.1"
                     /translation="MAEGKILENLWEAWRLFRLWLYLKWILLFRRLPRDAYQWITIEP
                     EGIRIQSFRPDNSRSQWDGEAYFEFIGMRARFCITMGSSKPSEAEFDFLRTLLRRKQS
                     FRHALAVEMFKHYQEYVFNHMDFRVRKEGKETYIPAPAVATPSALDNTVYSSSIYFSD
                     DDAGQSQFDIHVKCDLDVVLQSFTLRVKDWRVVRVE"
     gene            12141..12767
                     /locus_tag="VFV87_21005"
     CDS             12141..12767
                     /locus_tag="VFV87_21005"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEX5106318.1"
                     /translation="MAATSVSNHEPLLLAITGASGAVYAVRLLEVLVEGGQNVHLLIS
                     PSGAAVIQQELDRNVSVDRFELTQLLPAAIAAKAKDLVQYHHHADFLSPLASGSFLTG
                     GMVVCPCSGSTLSGIAHASSTNLIQRAADVHLKERRKLILVPRETPLSLIQIDNLRRA
                     AEAGAVILPASPGWYHGVRTPQDLVDFVVARILDQLAISHRLMRRWGE"
BASE COUNT         2528 a         4130 c         4006 g         2294 t
ORIGIN      
        1 agcggatgga atccggtggc ttgctgcttg agccagtcgt agtaggcctt ctgggccgcc
       61 ggctcgcgcg gcgcgttctc aaagcgcagc cactccgcca gccgatacgt ccagtattca
      121 atgaagtccg gcgaggcgat caagcgttcc acttccgcgt cgaatttgtg ttcgtcctga
      181 tcgctgagat actccagaac acggcccggc gccggcagcc ggcctgtcaa gtcgagcgtg
      241 acccgccgca ggagggtcgc gtcgtcggct cgtggggcgg gcggaatccg cagttcttgc
      301 agcgacgagt tgatctcgtc gtcaatccag ttgctccgcg gcatgtcggt ggttttgagc
      361 cttttgcctg gatacgggac ggtaatctgc attgccgcca cttgcgtgag atgacgcaca
      421 acaatggtgt ggcgtccggg actcaagacc tgcacgcgcc ccttgtcatc cacaaccgtc
      481 gccgcggggt cagctggcga aaagatagtc gactccgtta ccggccgggt gctgccgtcc
      541 gagaagcttg ccgacacatg cagtgtcaaa tgtcgtggca gcgcttgcga cggttcttcc
      601 gaatacgata tgcgaattcc aacgagcgtc ttcttggact cacgtagtgc gccggccgcg
      661 atccagtcag tcaggagctt tgcttgcggg ctgtcggctg cgaaccgctc gcccccttcg
      721 tggtcgagct gccaggtggg cttgagcagg agcaaactcc gctcaggatg ggccagattg
      781 acgcggcgac cttctaactc gtggacgatc tgccggtaat cggcggccgg atcgccgccg
      841 aagagcgaga gcttgaagcc gccccggccg gcggctgctc cgtggcacgc gccggcgttg
      901 cagccagctt tggccaacac cgggagcacc tccgtgtcga aatcgaccgg attgggcggc
      961 gcagccgcga gcagcaggat gatggcgcaa gcgttcacag ggctgaatcg accgaatgtc
     1021 cctcggctgg aaagtataac cctgcgcggc ggcaggtgtc gcgtgttttg cttacgggtg
     1081 caacgcgcta aaacagccgc aaggctgttc cagatccgca cggaatgccg atctcgccaa
     1141 ggtagctttc gattttctgg atatcgctgc agagggcatc gaggtccgca gggttcccgt
     1201 cgccgtcgca gatcgtccca ccgagccttc ttcgggcata ctgcgctgcc tcgatcatcg
     1261 cctcgaagac ttccacagga gcggcacacc gcggcacgga caaggagaac accaaatcgg
     1321 tcgtatggac gcggttggcg gcgatctctt caggcaggaa gtatccgggg ggcgttgaag
     1381 tctcgacgct aaaccaatgg tcgtctccca cctcggatgg attccgccaa tgaaagcagt
     1441 ccatatcgcc ccaccgcaaa cccaggcaga gcatgacgtc ccaaatctgc ttgccgctga
     1501 atttctgtcc gcggggagct tcgagcacca ggacggccgg atagttccat tcccgctgga
     1561 cttccttgag ggtttcgctg cgggctgccg cttcctctgg cgactggctg gcgctgacct
     1621 gcaccttgcc aaatgccgcg agccggcgtt cgacctcctt aagccgcgcc gcaaattgcg
     1681 ctgcagtcgt cgccgcctcg tcctcctcaa gcgggctggc gtagtcccag gccaatttca
     1741 attccgttac tgcggatggt ccatcggaag aaatcagata ggtccagcgt ccgtcggccg
     1801 cgtcgcgccc gtacagcgtc agcccgccaa accgctcccg ccagttccgg tcgaacaacg
     1861 cgttcacgtg actgggagcc agcttcggcc ggccggcgaa ctgcgcggtg atcacccagt
     1921 ccacggcaag ctcgggaagg taatctcgct ccgattcttc cggttttggc gacgggagat
     1981 cgatcgcccc aagcaagtgc gatacatctt cggacggttc ggtcggcaat agcgcggcgg
     2041 cgcgggagtc gctctcgctg gccggagcaa tgacgtattc gtcgggagaa ggttgatcgg
     2101 ggtgggacat atcgacacca catcccaagg aaaccgcgaa gagagcggca aaccagatca
     2161 cacggctgcc agcattgtgc atcgccggca tcatagtcgc cctgcgagtg gtttgcacca
     2221 ctgcatgcac taaggctcgc cgacctggcg gaggtactcc gccagacggg cctgcaaatc
     2281 gatcagcgcc tgccactgcc gcaggtcgag agcgaccttg ttcgatttgc tatcgacctg
     2341 caccgagccg atcaggttcc gcagccgcag gtgaaggaac tccaagagct ccgcgagctg
     2401 ggcggcctgg ccgggggtca ggcgctctgg caattcgggg ggctcgaggg catgaatcgt
     2461 cgcctgcagg tcggcgtctt cgctccagtt gagctcgaag tcgagcgacg agatgtttgc
     2521 cgccttctcc agctcctcgg gatctgcggt acggctggcg tcattctcgc cggccctcaa
     2581 gcgggccagc cgctgggcga tctgctcgcg cgacccaatc agcaggacgc tgcgccccag
     2641 ggcgatcatg tcgccgaatc gcagaatgcg gagctgcacg tcctcgccgt taatctttgt
     2701 accgttggtg ctctccagat cggtaatgac gaggcggtcg tggtcttcct ggatcttgac
     2761 gtgataccgg ctgacgcgct cgtcgttgag ctggatggta ttcccctctt cgcggccaat
     2821 ggtgatgggt gtatgcaagt tctgaaacac gcggccgcgg tcagcaccat cgagaacgcg
     2881 tatcgtgatg ccgctcatcg cccagccaga gagaaaagac aacggccgcg caccgcgaca
     2941 tcgtgaagcg gttggcggga ctctgttcct attctgcgct tatagcatcg cgggaattgg
     3001 ggggtcaagt cagtaggggc cagtcgtagg caactcgctc tgcgacgcac gatcgaacta
     3061 tctgccgcac aagacttgcc gtatgggagc cgtatcattt ctcgtggagc gagaaactta
     3121 cggtttacat cttctcgacg acttgaatcc ctaacagatc cagccccgtg cggatggtgc
     3181 gggcggtgag gtcgcagagg aggaggcggc tattgcggat ttcgtcggtt tcggctttga
     3241 ggacggggca gctctcgaag aatgcggagt agctcttggc gaggtcgaac aggtagccgg
     3301 tgagatggtg gggcatgtag tcggcttcgg tttcggcgag ggcctcggaa aaacggagga
     3361 gctggattcc cagggcccgc tcggcgggat gcgaaaggac gatcttcgcc ccgctggccc
     3421 ggacggcagc gggatcgaag ccccctttgg caaaaatgct gcgtacgcgg gcatagctgt
     3481 attgcatgta cgtcgccgtg ttgccggtca tcagcagcat tttgtcgtag ctgaaggtgt
     3541 agtcgctggt gcggttctgc gagagatcgg cgtccttaag cgccccgatg cccacgacct
     3601 cggcgatctg ctggcgctgc tctggcgtga ggtgattgtc ggaatcgttt tcagcgacga
     3661 ccgcggcggc ccgcctgacg gcttcgtcga tcagcgattc gaggccggcg gcgacccctt
     3721 cgcgcgtctt gtatggcttg ccgtcggcgc ccatcacggt gccgaacttg atgtgctgca
     3781 gctcgacttc cttgtggctc cacaggcggg cggcggcaaa gagcttgtcg aagtgctcgc
     3841 tctgccggtg atcgacgacg tacaggattg catccggctt ccaggtctcc atccggtact
     3901 ggatcgtcgc caggtcggtc gtcgagtaca aaaacgcgcc gtcgctcttc tgcacgatca
     3961 tcggggcttc gaagccctcc agaaagacgc acacagcccc ttcgctctcg cgcgccaggc
     4021 ccttggcccg caggtcggcg atgaccgcgg ccagccggtt gtgatagaag ctctcgccgt
     4081 attcgtggtc gaacttgacg ttcatccggc catagacgcg ggcgatttcg tcgcggcagt
     4141 tggggaggaa ttcgtgccag agcttcagat tctcctcgtc gccggagtgg agttttgccg
     4201 tctcggcgag gacagcctga tcgatgttcg gatgcttggc agccgcagcc gcgagctgcg
     4261 gatcgctttc caccgcggcg atcttctttt gcaatgattc gacttcttcg cgcgcagcct
     4321 tcacctggtc ctgagctttc gccagcgcct gggcgttctt cttatcgatc gctttgtcgc
     4381 cgattttcgg ttcggcttgc agagttgccg cagttgcttc gcgctgggca agccgctggc
     4441 gggcttccgg cagggccttc tggctcgcgc ggtaatcaac cagccgattg acgaacttgt
     4501 acagccgccc gagttccgcc acgggattcg cctcgaacgc cgcccggtcg acgaagtgct
     4561 tgtagccgta aatgatcatc ccgaactgcg tcccccagtc gcccaggtgg ttgtcgctga
     4621 tcacccggtg gccgaggaag cgcagcgtgc ggcagagggc gtcgccgatc accgttgagc
     4681 ggatgtggcc cacgtgcatc ggcttggcga cgttcggcga gctgtaatcg aggacgtacg
     4741 tccgcggctg gcgggccggc tcgatcccca gccgctcgtc atggaccgcc cggttgagct
     4801 gtccggtcag ccagtcgtcg cgcaatcgca ggttgatgaa ccccggccct gccacttccg
     4861 gcggcaggca gatgtctgca aggtccgtcg ctccaacgac ttgcgcggcg atctgcggcg
     4921 gcggccggtt cagctccttt ttgagcgtca tcgcgaaatt agcctgatag tcgccgaatt
     4981 tcgcgtcctg cgccggacgg accatgtcca gcagctcctc cagccgggcc ggatcactga
     5041 tcagctctgc caggaccgga cggaagcggg atttcaggag ggcgaggacg ttcatcgcag
     5101 gttggacctc cggtccgacc gaaatagcac ggacggacca gaggtccgtc ctacgggatc
     5161 cagcggaggt ccatcctaca agaaagagcc acgggcggct cgaccggttc gagccgcccg
     5221 cggcgggttg gtacatcgtc aggcaacgga actattcagc cgactcggcg ggcggggcac
     5281 tgtcctcaac cccttccgtg gcggatgccg tggtgacgct cgaatccttc gtcgtctcgg
     5341 cttccgccag ggcttcctct tcagcttggg cggctttggc aatctcctct tcggtctcgc
     5401 caacggcgtc tccgctggcc cggttggcat acacgcccac ccagtaacat tggccgctgg
     5461 gggaaatggc gtagccaaat ccggcgtcgc tcgtgttgct gacaatcgag gcataatgcc
     5521 cactgctgcc gacccacatg gcgaacgtgc tgtccaccga tcgcgagccg taagcgatgt
     5581 tctccagcac accgccgcgg aatccatact tgcccgcccg gtactgcggc ccgccgttca
     5641 cataatggct gaactgggcg gtttgggcca tgtaattggc ctggtcctgg gcggctttcg
     5701 tgagcgcggg attcagccga tggggccgca gccccagccg ccgccgcacg tcgttattcc
     5761 gcctcagcat tcccagcagc gtgggatgcc ggtgcaaagg tcgcctggcg gctgtcgagc
     5821 cttgagcgga cgaagcctgg cccgaaccag cggcaccgga gctcccttcg tcagccagcg
     5881 cgaccgacgt acagatcacg agccccaaag aaatcaagcg tctcatccat tccctccctg
     5941 caaacgagga acaactctgg tgggaaacca gaaggtggga acccaggcaa agtggcctga
     6001 gtttgcgggg cgaatttgcc agaagtggga gagtttacaa gtagcccaaa gccgccagaa
     6061 ccataaagac ccaccgatat gactgcgaca gcgagcgcca agatgcgata tattgaaaaa
     6121 atgggaaatc tgcgaagact gcggtgttct tggccaagga gatggaagct gctcttcact
     6181 tgggctccgc gctcgcggtg cggtggagaa gctggcgatc gcgcgggcca aagcgcctag
     6241 aaaccgcttc caccgggttg acgtccgctg ccggcattgc ccgagcaaaa gagcttcaaa
     6301 agtctccgca gaaaggctcg cccggcgcag gtcgtaggcg ccgcatcaca gcgatcgcca
     6361 atatcgcgaa taaggttgtc gcgcaccggc tctggcttcg caccggtcaa tcgcgaatct
     6421 cgcgtcatcc tcgcatttcc aggacaaggt ctccaccaac gagtgcggtg ccgacgcagg
     6481 tcaccggccc acccgccggc gattcacgtg gcacaatggc cgccaactcg ccataatgag
     6541 aaaagtccaa tcgcacccga aatccccggc tcctgaactg gagtaacgtc atgcgccgcc
     6601 tcacaagcta ctttgccgtc gcgctcatcg ccctggccgc taccgcccgt gccgagaacg
     6661 aaggccaggc cgatctcgac aaggccacgg agttgcaaat tgctgccgaa acgctggccg
     6721 acctggaaaa ggtcatcgaa ctgggcgaaa gcgccctgaa gaaggggctc gacgaaggcc
     6781 aaacggccct ggcgaagcaa attctcgcgg ccacgctgta ccagcacgcc gatcgccttt
     6841 cggcgtccat ctttgagcaa tcgcccccca gcccccgctg gccgctggtt cgccagttcg
     6901 ccctgcgtga catcgaaaag gccaagcggt acgacgcgaa attgcccgac ctgtttctcc
     6961 tggaggccaa gctgcaggtc ctgcccggcg gcgacgaaaa ggcctcgcac accgccatcg
     7021 acgaggcgat tagcctgctc gagaaaggcg acgataaaaa ggagctggcc aaggccctca
     7081 tcctgcgggc cgccatgacc gacgatgacg agcggaagct tgccgatttc aactccgcca
     7141 tcaaggccga tcccgagaat gccgaggcgt ggcaaggccg ggccttgctc tatatcgaga
     7201 aaggcgagag cgagaaggcg gtcgctgatc tgacgaagat cgtcgagaag aacaatagca
     7261 gtccgctggt catcggagcg ctggccgagg ccctgacgaa cctcaaaaag tacgacgaag
     7321 ccctgaagta ctgcgaggac gtgatcaagc tcgcccccaa atcgactctg ggctacaacc
     7381 tgcgggcccg catcctgatc atgcaggaca agaacaagga ggcgatcgag gacctcaatc
     7441 aggccctgca ggtcaatccc aacgatttgg ccgcgttgct cacccgcagc cgcctcaacg
     7501 ccgccgaggg caaggacgat ctggcgaaag ccgatgtcga aaaggccctg gccatcgatc
     7561 cggaactgcc gcagggcatt ctgctgcggg ccttgttggc cgctcagaag cagaaattcg
     7621 gcgaagccat cgccgatatc caggtgctcc tgcaaaccga tcccactaac ccggaatatc
     7681 gcctgcagct cgcgatgttc tacgtgggcg acaagcgccc gcggcgcgcg atcgacgcgc
     7741 tcacttcgat cctgggcggc ctgaccgacg acggcgacga ggaacaaaag gagattaagg
     7801 cccgcgccct gcaggcccgc ggcgacgccc tgctcagtgt tggcaagcat gccgaggcgg
     7861 ttgaagacta cgaaatcgcc ctcaagctcg agccagagga caccggcgtt ctcaacaact
     7921 ttgcctgggt gctggcgact tcgcccgacg acaacgtccg caacgccgcg cgctcgatcg
     7981 agctcgggac caaggcctgc gagctgacca agtacgagcg gccgcacatc ctcagcaccc
     8041 tcgccgccgg ctatgccgag aaaggcgact gggaaaacgc cgtcaagtgg tccaccaaag
     8101 cctccgagct gggggccaag gacccggaag tcggcgagca actcaagaag gagctggaga
     8161 gctaccagga aaagaagccc tggcgcgaga agcaggagat cgaagaaaac aaggccccgc
     8221 tggggaagtc gagcgatttg gaaacgtgag ccaagtgcgg aatgcggagt ccgaaatgcg
     8281 gagtaattca gcattctcgc gcgacgcgta ggttcaattc attgtctcca ttccgcattc
     8341 cgaattccgc actccgcact cgatggaccg ctcctccgcc ctcgccctga tgcacgagca
     8401 cacgcagtcg ctctcgctgc ggcggcacat gctggcagtc gaggcggcca tgcgggccta
     8461 cgcccagaag tacggcgagg atgaagagaa gtggggcatc gtgggcctct tgcacgattt
     8521 cgactacgag cgctggcccg atcctcccga tcatccactg cagggcgcag cgatcctggc
     8581 cgagcagggc tatcccgagg acgtgatcta cgccatcaaa tctcacgccg actacctccc
     8641 cgattgcccg cgcgtccatc tgatcgacaa agccctctac gcctgcgacg agctcgccgg
     8701 attcctcacg gcctgtgcga tggtccggcc cgaacggctg gagggcatgc agcctggcag
     8761 cgtcaaaaag aagctcaagg ccaagggctt tgccgccagc atcaaccgcg aggacatcgc
     8821 caagggcgcc gccgacctcg gcgtcgatct cgacgagcac atccacttcg tcatcgccgc
     8881 catggcggcg attgcagagg atcttggact atcggtcgaa ccgtcgccat cgtgagatcg
     8941 cccgctagtc ccccgtggta tccaagctcg gcagaattgg ccgatcgacg ccgccttctt
     9001 cggcgtggac ggaaatgacg cgaagggcgg gttggacgat ctcgatctgc ggctcggggg
     9061 cttgccgcaa gagcagcaca gtgcagagcc gggcgagcga cgagcagcca aagagcacga
     9121 ggtacgcgag atgcgattcg ctgccgagct gcagaatcgc cgcgccgatc aggccgccga
     9181 cgaccagagc cgccgcgttg ccgaagttgt aaaacgtcag cacgctggcc cggtcttgcc
     9241 gcgggatcgc ctcaaagaac atcagcagca tcgccagttc ataggcggcc caggcgaggc
     9301 cagaaatgag ctgcaccacg ccgatgtaga tcagtttgcc cgaaatcggg acgctgacca
     9361 cacccagacc aagatggaga tcgagctgcg aatgccatgg atcgaacaga tcggcaaaga
     9421 tccacagccc ggcgacgggc acgatcgacg ttccgccaat ccacagcagg cgtcgggcgc
     9481 cggccttctg cgcgacccgg ccccacagcg gcagagcgat cactttgccg agaaagccga
     9541 tgccgatcag caccatgtag ctgaagtagc tcagctcctg cttgaccagc atgaacggcg
     9601 tgaaatacgg gccggagatt tgcacggcag tctggaccgc gagcaggtag agcacaagcg
     9661 gcacgccgac atcgccgctg gtgcggaaga tcgcttcgcg gagcgagacg ctgcgggtct
     9721 cgtatttgcc gcacgacggc tcggtttgcg tgctcaggaa ccaggccgac aggaagcggc
     9781 aggcggagcc gatcaggaaa atgccagcga acgccgccag cagccagcct tgggctttgc
     9841 ccagctgcag cgcgatgcct ccggctacga agccgatgag cgtacacatc tggctggtgc
     9901 gggcccggca ggcgaagaag ttggcccgca cgcggcgggg aatgatctct tcgatccagg
     9961 tgttccacgc cgggccggtg gcctggctgg ccgcccaata ggccgccgcc gcaacgaaga
    10021 cccacagcgc cgcggccggt gtgcccatga acagcgcagc ggtgggcatg gcgaacaagg
    10081 ccgcggcttg caggctggca caaagcacga cccacgcgcg gtacgaacgc aagcggcgca
    10141 gcgcccacgg cgtggcgagc tgcagggaag ccccgacgag catcggcagc gtggcgatga
    10201 tgcccgcgaa agtctcgccc gtacccagga acaacgcaaa cgcggcgaaa taggtctcgc
    10261 cgatgccgac catcacgcta tacgaggcgg catcgccgat gctggcccgc atgtcgccgc
    10321 gcgggctgac gcggggcgcc cgcggctcgg cgaggagggc caccggcgag gtcgcgggcg
    10381 ctggtggaag gatttcaggc ggcgcttccc ggcgaacatt cgccggccgg ggaggcgggg
    10441 caagagacat ggaaggctca agtggggcgg gcagttacgc tatcatacgt tctgcggttg
    10501 cagtgtgtga gaggacagct aatagccatt tgcgttcacc gccacaatcg caaacgagcc
    10561 gatttgctgt aagctgaact ccggcaacga ctttcgtacg tccgcggcgg gcggttgact
    10621 tcacatcaac ccgtcgccgc cacaaagccg cgaccagtgg tcgcggttcc cgtagccgca
    10681 ctcgccagag tgcggggagc ggcttcgcag gcccgcgttc tggcgaacac ggctacattc
    10741 aaagcatgtc caccaccatc gacaaatcgg gcgagcgggt gcagcggatg ttcggcgaaa
    10801 tcgcgccgag ctacgaccgc atgaaccacc tgctctcgct gaacgtcgat cgctactggc
    10861 ggtggtggac ggtgaacaag ctgcagcccc agcccggcga gccgatcctc gatgtctgca
    10921 caggcaccgg cgacctggcg ctggccttcc accgcgcgac gggcggccag tcggaaatcg
    10981 tcggcgccga tttcacgccc gaaatgctcg aaatcgcccg ccgcaagcaa gcccgcgcca
    11041 agattggcga ggaactctcc tatctacaag ccgacacaca gaacttgccc ttcgacgacg
    11101 accgcttcgc catcgtcagc gtcgccttcg gcctgcgcaa cgttgccgac accgatcgcg
    11161 gcctcgccga gatgacccgc gtctgccgcc ccggcggaca ggtcgcaatc ctcgagttca
    11221 gcactccccg ctggcagccg ttccgcggac tgtatctgtt ctacttccgc cacatcctgc
    11281 cgcgagtcgg ccagctcttt gctcgcaacc aatccgccgc ctacgaatac ctccccgcca
    11341 gcgtcggcga gttcctgggt ggcgaagccc tcgccgagcg gatgcgcggc gtcggcctca
    11401 ggaacgtgaa atactggccg ctaaccttcg ggattgccac actgtatgta ggaaagaagt
    11461 agggcgggac cagcgagcaa agcgagcgca ggcccaacat ctttcgacgc cgaggctact
    11521 cgactcggac gacgcgccag tctttaactc tcaaggtgaa gctctggaga accacgtcca
    11581 aatcacattt cacatgaata tcaaactgac tctgtcctgc gtcgtcgtcc gaaaagtaaa
    11641 tcgaagagga atacaccgtg ttgtctagcg cgctaggagt cgcaaccgcg ggagcaggaa
    11701 tatacgtttc cttgccctcc tttcgaacgc gaaagtccat gtggttgaag acatactctt
    11761 gataatgctt aaacatttcg accgccaggg catgccgaaa cgattgcttt cggcgcaaga
    11821 gcgttcgcaa aaaatcaaac tccgcttcgc tcggcttgga actgcccata gtgatgcaga
    11881 accttgcacg cataccaatg aactcgaagt aggcttcgcc gtcccattgc gagcgagaat
    11941 tgtccggtcg aaagctttgg atccgaattc cttcgggttc gatggtgatc cactggtagg
    12001 cgtcccttgg aagtcggcga aacaatagaa tccacttcag atacagccaa aggcggaaaa
    12061 gtcgccacgc ctcccacagg ttctcaagta ttttgccttc cgccattgtc gcgcacgtct
    12121 cgcccggatt gtcacctgaa atggccgcta ctagcgtatc aaatcatgag cccctcctcc
    12181 tcgccatcac cggcgccagc ggcgcggtct atgccgtgcg gctgctggaa gtgctggttg
    12241 agggcgggca aaacgtgcat ctgctgatca gcccctcggg cgcggcggtg atccagcagg
    12301 agctcgaccg aaacgtcagc gtcgatcgat tcgagctgac gcagctgctg ccggcggcaa
    12361 tcgcggcgaa agccaaggac ctggtgcagt atcatcatca cgccgatttt ctctcgcccc
    12421 tggcgagcgg ctcgttcctc acaggcggca tggtcgtctg cccctgctcg gggagcacgc
    12481 tcagcggcat cgcccacgcg tcgagcacca acctgatcca gcgggcagcc gatgtccacc
    12541 tcaaggagcg ccgcaagctg atcctcgtcc cccgcgaaac gcccctctcg ctcattcaga
    12601 tcgacaacct gcgccgggca gccgaagccg gcgcggtgat cctccccgcc tcgcccggct
    12661 ggtaccacgg cgttcgcacg ccgcaggacc tggtcgattt tgtcgtcgcc cgcatcctcg
    12721 accagctcgc catctcccac cggctcatgc ggcggtgggg agagtgactc cgagcgtctg
    12781 aacggcaaac aggccgaagg gcgcgatcgc cctccggcct aggcaaacac ccttgcgaca
    12841 ggcaagcgtc ttgccccctc atttactcgc ttttcagtac gacaaaccgc gagccctgct
    12901 cggtgcggac aagcagtagc acgcctttct cgggcgacga actgcccatc gccgcgtt
//
LOCUS       DASUGB010001106         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1511045, whole genome shotgun sequence.
ACCESSION   DASUGB010001106 DASUGB010000000
VERSION     DASUGB010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511045"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="mch"
                     /locus_tag="VFV87_21010"
     CDS             complement(<1..771)
                     /gene="mch"
                     /locus_tag="VFV87_21010"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEX5106319.1"
                     /translation="MNLNDMAWRRCEELAAAAERLGVSISKSAAGTRIIDCGVKAPGG
                     LEAGRLLAEICLAGLGRVTYSPGRTGDWIGPVVAIHTDQPVLACMCSQYAGWQITGEK
                     FFGMGSGPMRSVACHEPLLEYLGYREKADRVVGVLETSKLPTDEVCEKIANQCSVTPD
                     RLTLLVARTASIAGTLQVVARSVETALHKMHELGYDLHKVVSGFGSAPLPPIAADDLV
                     GIGRTNDAILYCGEVTLWVRDDDEQLAELVQKIPSLASP"
     gene            877..1530
                     /locus_tag="VFV87_21015"
     CDS             877..1530
                     /locus_tag="VFV87_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5106320.1"
                     /translation="MASLTIENYVKAIYQLAPAKDRTAAATGKLAEALGVSPGTVTSM
                     LKTLSESGLAEYVPYEGARLTDAGRTLALRVLRRHRLIELFLVRTLNLSWDEVHEEAE
                     NMEHAVSDLLIDRIDAFLGYPAADPHGDPIPKADGTVARSSGRRLSQLAAGQRFRVVR
                     VVEQSPEFLRYLTETGLRLEAEGHVIANRAESGTITVEVAGQQTTLAHNIADRLLVT"
     gene            1550..2077
                     /locus_tag="VFV87_21020"
     CDS             1550..2077
                     /locus_tag="VFV87_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106321.1"
                     /translation="MLKMPYVMVDIESDGPIPGDYSMICFGAVIVEPALDRTFYGRLK
                     PISEKYIPEALAVSGFSREETLAFDDPASVMAAFRDWVKENCQGRCFFISDNNGFDWQ
                     FINWYLHHFVGENPFGHSSTNLGSLYKGLARDMFVNFKHLRKTKHTHHPVDDARGNAE
                     ALLAMKEMGLKISFE"
     gene            complement(2176..>2832)
                     /locus_tag="VFV87_21025"
     CDS             complement(2176..>2832)
                     /locus_tag="VFV87_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX5106322.1"
                     /translation="AKWAMTLDGKIATRSGYSKWISSEESREVAHALRGRVDAILVGR
                     RTAQIDDPLLTCRLKAGTPARRATRIVLDSLAQLASSSQLVRTARQYATIIACGPEAP
                     EKDLRRLTAAGCEVLPFAASTSYERLLLLLDELGRRQMTNIFVEGGATLLGALLDARQ
                     IDEVHAFIAPRLFGGQRAPSPIAGQGVDQPSACFELHNLAIRQIGTDLYVFGRTASQH
                     "
BASE COUNT          494 a          950 c          890 g          498 t
ORIGIN      
        1 gggagaggcg aggctgggaa tcttttgcac gagctccgcg agttgctcgt cgtcgtcgcg
       61 gacccacagc gtcacttcgc cgcaatacaa gatcgcgtcg tttgtgcggc cgatgccgac
      121 cagatcgtcc gcggcgatcg gcggcagcgg ggcggagccg aagccgctga cgaccttgtg
      181 caggtcgtaa cccagttcgt gcatcttgtg cagggccgtt tcgaccgagc gggcgacgac
      241 ttgcagcgtg ccggcgatgc tcgccgtgcg ggccaccagc agcgtcagcc ggtcgggcgt
      301 cacgctgcac tggttggcga tcttctcgca cacctcgtcg gtcggcagct tgctcgtttc
      361 cagcacgccg acgacgcggt cggctttttc gcggtagccc agatattcca gcagcggctc
      421 gtggcaggcg accgaccgca tcggcccgct ccccatgcca aagaatttct cgccggtaat
      481 ctgccagccg gcgtattgcg agcacatgca ggccagcacc ggctgatcgg tatggatggc
      541 gacgaccggg ccgatccagt ctcccgtccg ccccggcgaa taggtcacgc gcccgaggcc
      601 tgccaggcag atttccgcca agagccgccc cgcctccagc ccgcccggcg ctttgacgcc
      661 gcagtcgatg atccgcgtgc cggctgccga cttgctgatc gacacgccca gccgctcggc
      721 tgccgccgcc agctcttcgc accgccgcca ggccatgtcg ttcagattca tctccggtcc
      781 cctcgccccg taaaactagc ccctcccccg accgagtaca ataagccgcc actatcatct
      841 gtcctctgtc ttctgtcctc tgccccgctt ccgcccgtgg ccagcctcac gatcgaaaac
      901 tacgtcaagg cgatctacca gctcgcaccg gccaaagacc gcactgccgc ggccacgggc
      961 aagctggccg aggcgctcgg cgtctcgccc gggacagtca cgagcatgct caagacgctg
     1021 agcgaaagcg gcctggcgga gtatgtcccc tacgagggcg cccgcctgac cgatgccggc
     1081 cgcacgctgg ccctccgcgt ccttcgccgc caccggctga tcgagctgtt cctggtgcgg
     1141 acgctcaacc tttcgtggga cgaggtccac gaggaggcgg agaacatgga gcatgccgtc
     1201 agcgacctgt tgatcgaccg gatcgatgcc ttcctcggct acccggctgc cgacccgcac
     1261 ggcgacccga tccccaaagc cgacggcacg gtcgcccgct cctcgggccg ccgcctgtcg
     1321 cagcttgccg ccggccagcg attccgcgtc gtccgcgtcg tcgagcagtc gcccgagttc
     1381 ctccgctatc tcaccgaaac ggggctgcgg ctggaagcgg aaggccacgt catcgccaat
     1441 cgcgccgaat ccggcacgat caccgtcgaa gtcgccggcc agcaaaccac cctcgcccac
     1501 aacatagccg accggctgct ggtgacgtga agccgtcaat cgtatcggaa tgctgaagat
     1561 gccctacgta atggtcgata tcgagtccga cgggccaatt ccgggcgatt attcaatgat
     1621 ctgtttcggg gcggtcatcg ttgagccggc gctggaccgc acgttctatg gccgcctgaa
     1681 gccgatctca gagaagtaca ttcccgaagc cctggccgtg agcggcttta gccgcgaaga
     1741 gacgctggcg ttcgacgacc cggcgagcgt gatggcggcg ttccgcgact gggtgaagga
     1801 aaactgccaa ggccgctgct ttttcatttc cgacaataac ggcttcgact ggcagttcat
     1861 caactggtat ctgcaccatt tcgtcggcga aaatccgttc ggccacagct cgacgaacct
     1921 cggctcgctc tacaaggggc tggctcgcga catgttcgtc aacttcaagc acctgcggaa
     1981 gacgaagcac acccaccacc ccgtcgatga cgcccgcggc aacgccgaag ccctgctggc
     2041 aatgaaagaa atggggctga agatcagctt cgaatagggg gcaggcagtc gcttgtcttt
     2101 gtggtctgaa aaccagatcg gatgctggac cgcgtcaatt ccagtcggcc ctcgcgtcct
     2161 gcgacccaac ctgcatcagt gttggctcgc agttctcccg aagacgtaaa ggtcagttcc
     2221 gatctgccga attgccagat tatggagttc gaagcaagca gagggctggt caactccctg
     2281 gccggcgatg ggactgggag cgcgctgtcc gccgaagagc ctaggggcga tgaaggcgtg
     2341 aacttcgtcg atttggcggg cgtccagcag ggcgccgagc agcgtcgccc cgccttcgac
     2401 gaagatgttt gtcatctgcc ggcggccgag ttcatccaag aggagcagca gccgttcgta
     2461 gctggtcgag gcagcgaagg gaagcacttc gcaaccggcg gcagtcaggc ggcgcaggtc
     2521 tttttccggc gcttcggggc cgcaggcgat gatcgtggca tattgacgag ccgtgcggac
     2581 gagctggctg ctcgatgcga gctgggccag cgagtcgagg acgatgcgcg tggcgcggcg
     2641 cgctggagtc ccggctttca gacggcaggt gagcagcgga tcgtcgatct gagcggtgcg
     2701 acggccgacg aggatcgcat cgacgcggcc gcgcagggcg tgggcgactt cgcgcgactc
     2761 ctcgctcgaa atccacttgc tgtacccgct gcgggtggca attttgccgt cgagcgtcat
     2821 cgcccacttg gc
//
LOCUS       DASUGB010001107         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_400973, whole genome shotgun sequence.
ACCESSION   DASUGB010001107 DASUGB010000000
VERSION     DASUGB010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400973"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1036)
                     /locus_tag="VFV87_21030"
     CDS             complement(140..1036)
                     /locus_tag="VFV87_21030"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5106323.1"
                     /translation="MNARLDWLITLSATLLLTGMTVAAEGQPQVVMLWPAGAATLQGA
                     GEKEITVPPDAKPGERINSIKNVHNPLIEVHLPAADKATGTAVIVAPGGGHRQLVWGS
                     EGTDIAKFLNDLGVAAFILKYRLAQTPNFKYTVEGEALADTQRAIRIVRSRCQEWGVK
                     PDRIGVLGFSAGGALAALADIRFDRGKADATDPIDRASCRPDFVGLVYPGWAPMDITA
                     PKDAAPAFLTSAGLDDASHARQTVEFYNSLFAVRVPVELHIYARGGHGGGIKPRDGIP
                     FGTWQVRFSEWLADLGMLKHKE"
     gene            complement(1129..>3143)
                     /gene="tssH"
                     /locus_tag="VFV87_21035"
     CDS             complement(1129..>3143)
                     /gene="tssH"
                     /locus_tag="VFV87_21035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX5106324.1"
                     /translation="DLGLLQAGAGVKGEFESRLKSVIEEVKNSPTPIIVFIDEAHNLI
                     GAGGSGGQGDAANLLKPALARGEFRTIAATTWAEYKKYFEKDAALARRFQVVKVEEPD
                     ERTAVEMMRGLVATLEEHHQVPIDNEAVEDCVRLSHRYISGRQLPDKSVSLLDTTCAA
                     IALSQSSDPPAIQDLRRRIEQINIALRILGREEELGASHDERKQKLTAELSEAEARLA
                     ALTERCEQERDLVRQLADLRQKLTGEQNGSAKAKGDGKPADPKAAAGKTAEGKGAADA
                     KASKTPADAKASKTTADAKAAPPAPPPTPAEIAAWKKEYQSLRDKLAALQGESPLVHS
                     GVTPSAIAATVSSWTGIPLGRMVANEMKTVLNLKQLLEESIVGQSHALEEIAVTIKTS
                     RAQLADPTKPIGVFLLAGTSGVGKTETAIALASLLYGGEQNMTVINMSEFKEEHKVSL
                     LMGSPPGYVGYGEGGVLTEAVRRKPYSVVLLDEMEKAHPGVQDIFYQVFDKGSMKDGE
                     GRDINFRNCVILMTTNAGTDVIKSLCADPDTCPEPEAFAQAVFPKLLEHFKPAFLGRV
                     KVVPYYPLAPDVLRSIVALKLGKIKRRIYDNYRAEMKWTPAVVESIAKRCTESDIGAR
                     NIDNILTRTLQPELATEFLARMAAGEKIASVQVDVKPDGNLSYELK"
BASE COUNT          465 a         1030 c         1008 g          640 t
ORIGIN      
        1 ctgcaagttg tcaaagagcg tctgggaaag cgactcgcgc gggaggttcg ggtcctcgcc
       61 cgcacgttgt aagccgcggc cggcgacgcc accttcattg gccgccggct gaatctttcg
      121 gctggcccct gcggctgatt cattccttgt gtttgagcat ccccaaatcg gccagccatt
      181 cggaaaaacg cacctgccac gtgccgaatg gaatgccgtc gcgcggcttg attcccccgc
      241 cgtggccgcc gcgggcgtag atgtgcagtt cgaccggcac gcggacagcg aacagcgagt
      301 tgtaaaactc gaccgtctgg cgggcgtgcg aggcgtcgtc caggccggcg ctggtgagga
      361 aggccggggc cgcgtccttc ggggccgtga tgtccatcgg cgcccagccg ggatagacga
      421 ggcccacgaa atcgggccgg cagctggcgc ggtcgatcgg gtcggtggcg tcggccttcc
      481 cgcggtcgaa gcgaatgtcg gccaatgccg ccagcgcccc gccggccgag aagccgagca
      541 cgccgatccg gtcgggcttg acgccccact cctggcaccg cgagcggacg atgcggatgg
      601 cccgctgcgt gtcggcgagg gcctcaccct cgaccgtgta cttgaagttc ggcgtctgcg
      661 ccaggcggta tttgaggatg aacgccgcca cgcccaggtc attgaggaac ttcgcgatat
      721 ctgtcccttc agagccccac acgagttgcc ggtgcccgcc ccccggggcc acgatcaccg
      781 ccgttcctgt cgccttgtcc gcagccggca ggtgcacttc gatcagcgga ttgtgaacgt
      841 tcttaatcga gttgatccgc tcgcccggct tcgcatccgg tggaaccgtg atttcctttt
      901 ctcccgcccc ctgcagcgtc gctgccccgg ccggccacag catgaccact tgcggctggc
      961 cctcggcggc aacagtcatc ccagtgagta gcagcgttgc tgagagcgtg ataagccagt
     1021 ccaatcgggc gttcatggca acaagactcc gactattgaa tgcgcgggat ttgccgcagc
     1081 ggtggcgtcc atagtgtgat tgctcaattc tatggccgct gggtgtgcct acttcagctc
     1141 atagctcaga tttccgtccg gtttcacgtc cacttggacc gaggcaatct tctcgcccgc
     1201 ggccatccgg gcgaggaatt cggtggctaa ttccggctgc agcgtgcggg tcaggatgtt
     1261 gtcgatgttg cgggcgccga tgtccgattc ggtgcaccgc ttggcgatcg attcgacaac
     1321 tgccggcgtc cacttcatct cggcccggta gttgtcgtaa atccgccgct tgatcttgcc
     1381 cagcttgagg gccacgatgc tccgcaatac gtccggcgcc agcgggtaat acggcaccac
     1441 cttcacgcgc cccaaaaagg ccggcttgaa gtgctccagt agcttgggga agacggcctg
     1501 ggcaaaggct tccggctcgg ggcaggtgtc gggatcggcg cacaacgact tgatcacgtc
     1561 ggtcccggcg ttggtggtca tcaaaatcac gcagttgcgg aagttgatgt cccgcccttc
     1621 gccgtccttc atcgacccct tgtcgaacac ctggtagaaa atgtcctgca cccccggatg
     1681 ggccttttcc atctcgtcga gcagcacgac gctgtacggc ttgcggcgga cggcctcggt
     1741 cagcacgccc ccctcgccat agccgacgta gcccggcggc gagcccatga ggagcgaaac
     1801 cttgtgctcc tccttgaact ccgacatgtt gatgaccgtc atgttctgct cgccgccgta
     1861 aaggagcgac gcgagcgcaa tggccgtctc cgtcttgccg acgccgctcg tccccgccag
     1921 gaggaagacg ccgatcggct tggttgggtc agcgagctgc gcccgcgacg ttttgatcgt
     1981 cacggcgatt tcttccagcg cgtgcgactg ccctacgatc gactcctcca ggagctgctt
     2041 caagttgagc accgtcttca tttcgttggc gaccatgcgg cccagcggaa tgccggtcca
     2101 tgacgagacg gtggccgcaa tcgccgaagg cgtgacgccc gaatgcacca gcgggctttc
     2161 cccttgcagg gcggccagct tatcgcggag cgattgatac tcctttttcc aggcggcgat
     2221 ctcggcgggc gtcggcggcg gggcaggtgg agcggctttg gcgtcggcgg tcgttttgga
     2281 tgctttggcg tcggcgggcg ttttggacgc tttggcgtcg gccgctccct taccttccgc
     2341 cgttttgccc gccgcggctt ttgggtccgc gggtttgccg tcccccttcg ctttggccga
     2401 gccgttttgc tcgccggtca gcttttgccg caggtcggcc agttgccgca ccaggtcccg
     2461 ttcctgttcg caccgctcgg tcaaagccgc cagccgggcc tcggcttccg agagctccgc
     2521 ggtcagcttc tgttttcgct cgtcgtgact ggcgcccagc tcttcttcgc ggccgagaat
     2581 ccgcagggcg atattgatct gctcgatcct ccgccgcagg tcctggatgg cgggcggatc
     2641 ggagctttgg ctgagggcga tggcggcaca ggtggtgtcg agcaggctga ccgatttgtc
     2701 cggcaactgg cggcccgaga tgtagcggtg cgacagccgc acgcagtcct cgaccgcttc
     2761 gttgtcgatg ggcacctggt ggtgctcttc gagcgtggcc accaggcccc gcatcatttc
     2821 gacggcggtc cgctcgtcgg gctcttcgac tttgacgacc tggaagcggc gggccagagc
     2881 ggcgtccttc tcgaagtatt ttttgtattc ggcccaggtc gtggcggcga tcgtgcgaaa
     2941 ttcgccgcgg gccagggccg gctttagcag gttcgccgcg tccccttggc cgccggagcc
     3001 accggccccg atcaggttgt gggcctcgtc gatgaagacg atgatcggcg tcggcgagtt
     3061 cttcacttcc tcgatgaccg acttcaggcg cgattcgaac tcccccttca cgcccgcgcc
     3121 ggcctgcaac aagcccaggt cga
//
LOCUS       DASUGB010001108         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_541126, whole genome shotgun sequence.
ACCESSION   DASUGB010001108 DASUGB010000000
VERSION     DASUGB010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541126"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFV87_21040"
     CDS             <1..265
                     /locus_tag="VFV87_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106325.1"
                     /translation="INTEMMTAVNSYIESLLNSLPQRENAQGFKNFMQRFLTITIYVS
                     VGGFYVLMLAKAAVYLFGLIYLRKPHIRELYQTVPAKLAKPAQ"
     gene            278..784
                     /gene="coaD"
                     /locus_tag="VFV87_21045"
     CDS             278..784
                     /gene="coaD"
                     /locus_tag="VFV87_21045"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5106326.1"
                     /translation="MTQSDSRVAVYTGSFDPITLGHLNVIERAAKLVDRLIVGIGINV
                     EKAALFSPEERVALVGQTTAHLKNVEVRAFANLAVDFVRECGSRVMIRGVRPLTDLAG
                     EFTMMMANRHLDPGIETVFLMADEEYSHVSSTLIKQITPLADDDMLARFVPRPIIPAL
                     RAKIGRQK"
     gene            complement(816..1502)
                     /locus_tag="VFV87_21050"
     CDS             complement(816..1502)
                     /locus_tag="VFV87_21050"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106327.1"
                     /translation="MDRPEGRKRPWLQFSLRTLFILTIGIALGFAPLKVWELTAPAKQ
                     QVTLETKMLEVPAGLVAELGLTLSRDGLTGAPRIVDLAELERQIATLKTRGAKVVAEP
                     TLCTLSGRPSKFTMGGEVPVPVTDENGLSTVEYREVGIGVEFVPTILRNGRINLALDT
                     TDSRVKPQLSTSLSGFTVPQQIDSVSSHTEFELADGETVLLGGLAIDPDRTSQRGVLV
                     VAATVRKMRR"
     gene            complement(1622..2776)
                     /locus_tag="VFV87_21055"
     CDS             complement(1622..2776)
                     /locus_tag="VFV87_21055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5106328.1"
                     /translation="MAWRSLTLRGLGYFNDAAAAQCELPRGHIPALDAIRGLAIVLVT
                     LYRFGGGADGVARAIDSPWLIGLGARGVDLFFVLSGFLITGILVSAKGKEGYFRNFYI
                     RRALRIFPLYYGALVLILVVAPFVSGKMPASLGEQAWLWLYGANVLQSIRGAWCLGSL
                     NHFWSLAVEEHFYFVWPLVIFLCPRPVALRVCGCVIAASALARAGWLAAGGNDVAAEV
                     FTLLRMDGLVLGGWLALVARSPGGLAWLVRLARPALVLSGACAFGTFLLGKRLLGLPD
                     LVWAIFFGSLLVLVVSSARESWLARWGNSSTLQFFGKYSYAMYVFQLPLIYGLAWLVT
                     APGLAYWFDSPLLGQFAYCVIMFGFTTLLALASWHCFEKHFLALKHRFGG"
     gene            2964..3548
                     /locus_tag="VFV87_21060"
     CDS             2964..3548
                     /locus_tag="VFV87_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5106329.1"
                     /translation="MATETTLTRSPELMNRDDTALLVVDVQAKLLPLIPGHERLVWNI
                     RRLIDGAKILGVPVAATEQYPQGLGGTAPELAQRLGTIPSKTAFSCGECGGIFTAWRD
                     KGIWKILVCGIETHVCVGQTAHDLLGEGFRVYVAVDAVAARGQLDHDIALRRMDSSGA
                     TLTTTEAALFEWCGQAGAPEFKQISQLVKEQPPS"
     gene            3639..>4247
                     /locus_tag="VFV87_21065"
     CDS             3639..>4247
                     /locus_tag="VFV87_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106330.1"
                     /translation="MCVFRGWLVAVMFVWLTGLIGCGTGIPGLDPNVEVVEHTDYNPP
                     PPKDEDGLADDKLEDKQTSFDPELVDRRPLGEWSINQSEAVIRLDVPLARPDSDAELL
                     MLRPSYADSLKAAKAHRSWISVLPSVNLLDGKAKQFDDGLYAALDQAYFQGHSEKLPS
                     HLDLVERMLAKVDAGSPAAAYLAAGLSLGGRETTVGNSAQRDK"
BASE COUNT          847 a         1317 c         1365 g          718 t
ORIGIN      
        1 catcaacacc gaaatgatga ctgcggtgaa cagctacatc gagtcgctgc tgaattcgct
       61 cccgcagcgc gaaaacgcgc agggcttcaa gaacttcatg cagcggttcc tgacgatcac
      121 gatctacgtt tcggtcggcg gcttttacgt cctgatgctg gcgaaagccg ccgtctatct
      181 tttcgggctg atttacctcc gcaagccgca cattcgggag ttgtaccaga cggtgcctgc
      241 caagcttgcc aagcccgcgc aataagcttc ccgccccatg acccaatccg actcgcgcgt
      301 cgccgtttat accggttcgt tcgacccgat cacgctcggc catctgaacg tgatcgagcg
      361 ggccgcgaag ctcgtcgatc ggctgatcgt ggggatcggc atcaatgtgg agaaagcggc
      421 cctcttctcg cccgaggagc gggtcgcact ggtcggccaa acgacggcgc acctgaagaa
      481 cgtcgaggtg cgggcgttcg ccaatctggc ggtcgatttc gtccgcgagt gcggctcgcg
      541 ggtgatgatc cgcggcgtac gtccgctgac cgacctggcc ggcgaattca cgatgatgat
      601 ggccaaccgg cacctcgatc cggggatcga aaccgtcttc ctgatggccg acgaggagta
      661 ctcgcacgtc agcagcacgc tgatcaagca gattaccccg ctggccgacg acgacatgct
      721 cgcccgcttc gtcccgcggc cgatcatccc cgccctgcgc gcgaaaattg gccggcagaa
      781 atagcactag taccgggatc tgtggtcgcc gccccctacc gacgcatttt gcgtacggtg
      841 gccgccacga cgagaacgcc gcgttggctg gtccgatcgg ggtcgatggc caggccgccc
      901 agaagcaccg tctcgccgtc ggctaactcg aactccgtgt ggctggagac gctgtcgatc
      961 tgctgcggaa cggtgaagcc cgatagcgaa gtgctcagct gcggctttac gcggctgtcg
     1021 gtcgtatcga gcgctagatt gattcgcccg ttccgcagaa tcgtcgggac aaattcgacg
     1081 ccgatgccga cttcccgata ctcgaccgtt gatagtccat tttcgtctgt cacagggacg
     1141 ggaacctcgc cgcccatcgt gaacttcgat ggcctgccgc taagcgtaca cagcgtcggt
     1201 tcggccacaa ccttggcacc acgagtcttg agcgttgcaa tctggcgctc taattccgcc
     1261 aggtccacaa tccttggcgc tcccgtcaat ccgtcgcgag aaagggtcaa gccgagctct
     1321 gcgacaagcc ccgccggaac ttccagcatc ttcgtctcca aagtcacctg ctgtttggcg
     1381 ggggcggtca gctcccagac tttcagcggc gcaaagccga gcgcgatgcc aatggtgagg
     1441 atgaagagcg tccgcagcga gaactgcagc cagggacgtt ttcggccttc ggggcgatcc
     1501 atagccacct ccatgcgcgc gaccagacag cgccgccaga atagtcccca atcgccgcag
     1561 atcgcaatgg gactttgttc gccaggggcg gccgctgtcg cctcggcatt cacagtcaaa
     1621 gctaaccacc gaaacggtgc ttgagggcga ggaagtgctt ttcaaagcaa tgccagctcg
     1681 cgagggccag gagtgtcgtg aagccgaaca tgatgacgca ataggcgaac tggccgagca
     1741 gcgggctgtc aaaccaataa gccaggccgg gggccgtgac gagccacgcc aggccgtaga
     1801 tgagcggaag ctggaagacg tacatcgcgt agctgtattt gccgaagaac tgcagcgtgg
     1861 acgaattgcc ccagcgggcc agccaacttt cgcgcgccga gctgaccacg agcacgagga
     1921 gcgagccaaa gaagatggcc cagaccaggt ctggcaggcc aagcagccgc ttgccgagga
     1981 ggaaggttcc aaaggcgcac gcaccgctga ggacgagtgc cggtctggca agccggacga
     2041 gccaagccaa tccacccggc gagcgggcga cgagcgccag ccagccgcca aggaccaggc
     2101 cgtccatgcg caggagcgta aagacttccg cagcgacatc attgccgccc gctgccagcc
     2161 agccggcgcg ggccagcgcc gaggcggcga tgacgcagcc gcagacgcgg agcgcgaccg
     2221 ggcgcggaca gaggaagatc accagtggcc agacgaagta gaaatgctct tcgacggcca
     2281 gcgaccagaa gtggttcaag ctccccaggc accacgcgcc gcgaatcgac tgcaagacgt
     2341 tggcgccata cagccacagc cacgcttgct cgcctagcga ggcaggcatc ttgcctgaca
     2401 cgaacggtgc gaccacgagt atcagcacca gcgcgccgta atacagcgga aaaatccgca
     2461 gcgcccggcg gatgtagaag ttgcggaagt agccctcctt gcctttagcg cttacgagaa
     2521 tgcccgtgat caggaatccc gataagacga agaacaaatc gacgcctctg gcgcccaggc
     2581 cgatcagcca gggcgaatcg attgcccgtg ccacgccatc cgctccgccg ccaaagcgat
     2641 atagcgtcac cagcacgatc gccagcccgc gaatcgcatc cagggccgga atgtgcccgc
     2701 gcggcagctc gcactgcgca gcagcggcat cgttgaagta cccgaggccg cggagagtaa
     2761 gcgatcgcca ggccatcggt gttgcaagcg gcgaggtgct gggtggcgcg gaaagcgatc
     2821 aaaaaggcta cgtcattcct tcccgcgtgc aggacggcga ctggcgacga cagccggttg
     2881 taacgaagag aacgcccatt ccgcctacaa tgcgccctcg gcctgcgttt cgcacgtgac
     2941 gtgggtcggt tattgtgaat cacatggcca ccgaaactac gttgactcgc agtcccgaat
     3001 tgatgaaccg cgacgacacc gcgctgctcg tcgtggatgt ccaggcgaag ctgctgccgc
     3061 tgattccggg gcacgagcga ttggtgtgga acatccgccg gctgatcgac ggcgcgaaga
     3121 ttctgggagt gccggtggcc gcgacggaac agtatccgca aggactgggc ggtaccgcgc
     3181 cggagctggc ccagcggctg ggcaccattc cgagcaagac cgcctttagc tgtggcgagt
     3241 gcggcgggat ttttacagcc tggcgggaca aggggatttg gaagattctc gtgtgcggta
     3301 tcgagacgca cgtgtgcgtt gggcaaaccg cgcatgatct gctcggcgaa ggctttcgcg
     3361 tgtatgtcgc cgtcgatgcg gtcgccgctc gcggacagct cgatcacgac atcgccctcc
     3421 ggcggatgga ttcctcgggc gcgacgctta cgacgaccga agccgccctg tttgagtggt
     3481 gcggccaagc cggcgcgcca gagttcaagc agatcagcca gctcgtgaag gagcagccgc
     3541 cgtcgtagtc ttcggagctt gccgcggccg ggaatattcg gcgtcccatg cggatcgata
     3601 ctggcagcgg cttcgattct attgtcggga ggtgtgccat gtgcgtgttt cgaggctggt
     3661 tggtcgcggt gatgttcgtt tggctaacgg gattgatcgg ctgcgggacg gggattccgg
     3721 ggctcgatcc gaacgtcgag gtcgtggagc ataccgacta caacccgccg ccgccgaaag
     3781 acgaggacgg gctggccgat gacaagctgg aggacaaaca gacgtcgttc gatccggagc
     3841 tggtcgatcg ccggccgctg ggcgagtggt cgatcaacca gagcgaggcg gtcatccggc
     3901 tggatgtgcc gctggcgcgg cccgattcgg atgccgagct gctgatgctg cggccgtcgt
     3961 atgccgattc gctcaaagcg gccaaggcac accgctcgtg gatttctgtc ctgccgtcgg
     4021 tgaatctgct tgacgggaag gcgaagcagt tcgacgacgg gctctatgcc gcgctcgacc
     4081 aggcgtactt ccaggggcat tccgagaagc tgccgagcca cctggacctg gtcgagcgca
     4141 tgctggcaaa agtcgatgcc ggcagccccg cggcggctta tctggcagcc ggcctgtcgc
     4201 tgggaggccg agaaacgacg gttggcaata gcgctcaacg tgacaag
//
LOCUS       DASUGB010001109         8378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1511315, whole genome shotgun sequence.
ACCESSION   DASUGB010001109 DASUGB010000000
VERSION     DASUGB010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511315"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFV87_21070"
     CDS             complement(<1..546)
                     /locus_tag="VFV87_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106331.1"
                     /translation="MKRLAKVMLLPAVLLPTVALALAVAPPTAAIAQTVKPVAVVSLA
                     SVEENLADVAYITRIAGQEDVGKTAMLFGNALTAGIDKSRPGGMYVVPQAGDFHAVAF
                     VPVSNLKLLLEVHKEQVGEPEDVGGNILKIGMDRTAYVKEVGGWAFVAESKEHLSNLP
                     QDPAALLGDLPKTYNVAGRVLL"
     gene            complement(703..924)
                     /gene="infA"
                     /locus_tag="VFV87_21075"
     CDS             complement(703..924)
                     /gene="infA"
                     /locus_tag="VFV87_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5106332.1"
                     /translation="MAQKEDAFVVSGTVTQALANTRFRVQLETGTEVLAHVAGRMRKN
                     FIRIVPGDKVNVELSPYDLSKGRIIYRER"
     gene            1146..1430
                     /gene="rpsR"
                     /locus_tag="VFV87_21080"
     CDS             1146..1430
                     /gene="rpsR"
                     /locus_tag="VFV87_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5106333.1"
                     /translation="MAFPSRFKPKDAKTRKRPKPKLKVKKKDPIWVDGVRPRPMFVDY
                     KDIELLKKLVNRHGKIVSRRKSGCTAVSQHAVALAIKRARFMALLPYVGE"
     gene            1507..1926
                     /locus_tag="VFV87_21085"
     CDS             1507..1926
                     /locus_tag="VFV87_21085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5106334.1"
                     /translation="MSTAFITSRRVEFSDTDAAGIMHFVAFFRMMEQAEHDLLRSVGL
                     SVISHETGGTLSWPRVAAKCEFQSAARFEDVLQIEVRISRLGTTSLTFAHHFALGGRE
                     VATGEITTVCCRMSATAPPQPMPIPADIAAKLRGFVV"
     gene            complement(1998..5060)
                     /locus_tag="VFV87_21090"
     CDS             complement(1998..5060)
                     /locus_tag="VFV87_21090"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEX5106335.1"
                     /translation="MIRTLGCSLAVSLVIVTCASAEEAGLPAAAQAFGKSRPAVVVQP
                     DVLIVCEGEEFQVRAPGWETKAFGTNYYAATLANTFLSRQAYLGAPEQCDKTVAEIAV
                     EIPAAGKYLTLVRYEAAYRFQTQFRVQVEQGGKLALDRLYGTRDNLKIWAFSQKLKTE
                     LQWSWGAGESIVWEGHDAAADLQPGRAIIRLIADKQPKPAAKRNIDLVMLTTKAEEVA
                     ERIEKESYLPLDGMLTQSGDIYLKVTNQSTQPLTITAGGNTGGLGTEHSPYWIHQRRW
                     GKFGPLMVTAGQTTDWVEIGSWIDSLNHGQLELVAGGEGLKYRYEFGVKNAAGAIESI
                     GTFDGTVERLPLAYFGDTRYRRRVIHQADVLYELLAHLKQQPMHGKAPVRTPIYGYTF
                     TPLEGDAKHAAAVEEFRRLFSLAGARGQEDDRLPGLEGPRGYIDVRGVATDKLAEYCQ
                     QLGERAKDIRVVSQGDEIGLPEPPGASDPAVFREWLKGQGITAGSLGLTPELAVYSPA
                     ENMKAANPGLYYWSKRYQYDFGIREIKKRTDILRQHLPSAAIGANFSPHHGGQEHAYL
                     GEVFKWVTCFRQDGMTMPWSEDYIFQVPAGSQQMNNISLDLARAANRGKTDRESMFYV
                     MPHWPGNTPDNWRRMFFGAVQHGATMINLFEFRPVQVAYTENHCSHPEMYAQVLRSFR
                     ELGTFEDIVQDGQVRPGIAGLWFSETGDIWRDNLGSFAAAKRGLYTAIVHQQLPLDFV
                     VEQDALDGTLAKYQVLYLTDRHVSRGASGKIAEWVAGGGRLFATAGAGMFDEFNQPNK
                     ILRELLGVEQTALEEPGPQIDYIKQDLAFVEPIETATWKHGDDDESIRVFCIKSTFMT
                     SGSAESLATFSDGSPAVTSRAVGKGQAIYCGFLPALSYYQPAIPQRPVDRTSAEDSLA
                     HLIPTKFDAAAYELIGLPAERIRRPVECSNPRVETSVIESPQGIVIPLVNWSGQPVKR
                     LVVTPGFAVPGGKITLASGQSPAIASEEKGLVSITLDLDVADAIILRK"
     gene            complement(5186..6691)
                     /locus_tag="VFV87_21095"
     CDS             complement(5186..6691)
                     /locus_tag="VFV87_21095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5106336.1"
                     /translation="MTTWLYVLITLVAAVAGMAVAWVAARWYLVLSEEEPDEQAQFAQ
                     ETLSRLQELTRKVKADVDQHSSCVEEIQAQLSQSLESDDESVLAAVSDLIEANQRMQR
                     QLDTAEERLQAQARQIESQAAEARTDALTQLANRRAFDDELRRCAADHQRRGMPATLI
                     LFDVDHFKRFNDTHGHQTGDEVLRGVARVLRSSLGDMGLVARYGGEEFAVVCSGLSYE
                     SIVGQAERARQAVAGTQLRTGGKSLSVTLSSGLADLLPGDDEKEIVRRADEALYSSKK
                     AGRNCAHYNDGRTNHFIKPEQARILPPVPIAASSVGDEWLFESEESTEVLAREPIPQV
                     SSRPAFFDDLIRRLSQWRRGRTPLALLLVQVDSLPRIVNDHGPSASEVVLRVTAQLIN
                     AVMRDMDHVARLGEDTFALILPGALLADSVGIAERLRHAVERCRLPRKAGASWFTVSI
                     GVVEASDGDDMRQILQRGRMALAAAVNQGRNCVVGHDAVGAPVRTPEMAVT"
     gene            complement(6927..7787)
                     /locus_tag="VFV87_21100"
     CDS             complement(6927..7787)
                     /locus_tag="VFV87_21100"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5106337.1"
                     /translation="MSAPYTAALLAELVGVNAARVRAWQRRGWLVPVEETHRLATFDF
                     TELSVARQLAELTRAGASTRLIARKLAEIHRHFPDVARPLAELSLVLDGRKLLVRRGT
                     GLVETSGQLRIDFDALDEDDPADEPPATIASPAIFLARQSPALPGELNVAHAAPEQLA
                     AWAADLDEAGDLRAAAETYRAALAAGGPQPELCFQLAEVLYRAGDLAAARERYFMALE
                     LDEKELAAAAFEGTLQSHEPYPDAHYHLARVLDELSRPEEAAAHWSRFLELSPESPWA
                     DEARLRLQVD"
     gene            complement(7830..>8378)
                     /locus_tag="VFV87_21105"
     CDS             complement(7830..>8378)
                     /locus_tag="VFV87_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106338.1"
                     /translation="AKCWLCGRELVLTADVVDEASESPFAASWSPRVGVEPRPFQFSL
                     ESLLLVITLAAVCLGAFVATPGLGVLALIVAAPALLRTVYEGHQARRRGKAMDVSEKL
                     LSFMASAGIALAALTAAAGAFFAACTASILAVCFAAEANRGQALGSGTENTIFIVCAI
                     VCVAASLAAFASIFWLTWPKRG"
BASE COUNT         1448 a         2903 c         2577 g         1450 t
ORIGIN      
        1 caagagcacg cggccggcga cgttgtacgt cttcggcaag tcgcccaaga gggctgccgg
       61 gtcttgcggc aggttcgaga ggtgctcctt gctttccgcg acgaacgccc agccgccgac
      121 ttctttcaca taggccgtgc ggtccatccc gatcttcagg atgttgcccc ccacgtcctc
      181 cggctcgccg acctgctcct tgtgaacttc caggagcagc ttcaggttgc tgacgggaac
      241 gaaagcgacc gcatggaaat cgccggcctg cggcacgacg tacatgccgc cgggacgcga
      301 tttatcgatc ccagccgtca gggcgttgcc gaagagcatc gccgtcttgc cgacgtcttc
      361 ctgcccggcg atccgcgtaa tgtatgccac gtcggccagg ttctcctcga cgctggcgag
      421 agagacgacg gccaccggct tgacggtttg ggcgatggcc gccgtcgggg gcgcgaccgc
      481 caaggccagc gccaccgtgg gcagaagcac agcgggcaga agcatgactt tcgcgagacg
      541 tttcacagga ttctccttag gaaattcgaa agggcagggg ggcgccgccc agaaaagtgc
      601 aaatcgggga ccgaaccacg gtataacccc agcggctggg ttggggtttc agttacccgt
      661 gtccaaagcc gcgaccattg gtcgcggttc gctgcgagcg gtttaccgct cgcggtagat
      721 aatccgcccc ttggacaggt cgtagggcga gagctccacg ttgactttgt cgcccgggac
      781 gatgcggatg aagttctttc gcatccggcc ggcgacgtgg gccagcacct ccgtgccggt
      841 ttcgagctgc acgcgaaagc gcgtattggc cagcgcctgc gtcaccgtgc cgctgacgac
      901 gaatgcatcc tctttttggg ccatacaggc tcctcaaggc gttatctgac aacaacttac
      961 tagcgtcaat atacggctcg ccgggcggtc gtaaaggcgg gatttggtga aatgcaccag
     1021 cggggtggct tgcgttcggg cgggccaatc ctagaatgag gggcttgctt gacccattgc
     1081 ctgccgcgct ggcgaggttt gtccgcgcaa ctgccttccc gacaatgttt tgcgattagc
     1141 acgccatggc ctttccttcc cgcttcaagc ccaaagacgc caagacccgc aagcgcccca
     1201 agcccaagct taaggtcaag aagaaggacc cgatttgggt cgatggcgtc cgcccgcgcc
     1261 cgatgttcgt cgattacaag gacatcgagc tcttgaagaa gctcgtcaac cgccacggca
     1321 agatcgtcag ccggcgcaag agcggctgca cggcggtgag ccagcacgcg gtggccctgg
     1381 cgatcaagcg ggcccgcttc atggccctgc tgccgtacgt gggcgagtag ccatcgtcgc
     1441 ctttcgctcc gcgaaagggc gcgttcgttc gcggagggaa cgacgacatt gagcgaggtt
     1501 cgttccatgt ccaccgcctt catcaccagc cggcgcgtgg aattcagcga caccgatgcc
     1561 gccggcatca tgcacttcgt cgccttcttc cggatgatgg agcaggccga gcacgatctc
     1621 ctgcggagcg tgggcctgag cgtcatctcg cacgaaacgg gcggcacgct gagctggccg
     1681 cgcgtcgccg cgaagtgcga gtttcaatcc gccgcccggt tcgaagacgt gctgcaaatc
     1741 gaagtccgca tctcgcgcct gggcaccacc agccttacct tcgcccacca cttcgcgctg
     1801 ggcggccgcg aagtcgccac cggcgaaatc accaccgtct gctgccggat gtccgccacc
     1861 gccccgcccc agccgatgcc gatccccgct gacatcgccg ccaaactccg cggcttcgtc
     1921 gtttaacgcg cagccgcagg cgtagcgcga gtgacggggc gccttcggct gcccgttaaa
     1981 cgatcgcgcg ctgaaactca ctttcgcaga atgatcgcgt cggcgacgtc gaggtcgagg
     2041 gtgatgctga ccaggccctt ttcttcggat gcgatagccg ggctctgtcc gctggccagc
     2101 gtgatcttcc cgcctggaac cgcgaaaccg ggcgtcacta cgaggcgctt caccggctgg
     2161 ccgctccagt tgactagagg aatgacgatc ccttgcggcg attcgatcac gctcgtctcg
     2221 acgcgcggat tgctgcactc gaccggccgg cggatgcgtt cggccggcag gccgatcaac
     2281 tcgtaagccg ccgcgtcgaa cttcgtcgga atcaaatgcg ccagcgaatc ctcggcgctc
     2341 gtccggtcaa cgggccgctg gggaatcgct ggctggtagt acgacagagc cggcaggaag
     2401 ccgcagtaga tcgcctggcc tttaccgacc gcgcgacttg tcacggcagg tgaaccatca
     2461 gagaaggtgg ccaaagattc tgcgcttccg ctcgtcataa aggtgctctt gatgcagaaa
     2521 acgcgaatcg actcgtcgtc gtcgccgtgc ttccacgtcg ccgtctcgat cggctccacg
     2581 aacgccagat cctgcttgat gtaatcgatt tgcgggccgg gctcttccaa ggccgtttgc
     2641 tcgacgccca agagctcacg caggatcttg ttcggctgat tgaactcgtc gaacatcccg
     2701 gctcccgcgg tggcgaacag ccggccgcct ccagcaaccc actcggcgat cttgcctgat
     2761 gcaccgcgcg agacgtgccg atcggtcaga tagagcacct ggtacttcgc cagcgtgcca
     2821 tcgagcgcgt cctgctcgac gacgaaatcg agcggcaact gctggtggac gatcgccgtg
     2881 tacagcccgc gtttggccgc ggcgaacgag ccgaggttgt cgcgccaaat gtcgccggtc
     2941 tcgctgaacc agaggccggc gatgcccggc cgcacctggc cgtcctgcac gatgtcctcg
     3001 aacgtgccca gctcgcggaa gctccgcagg acctgtgcat acatctcggg atgcgagcaa
     3061 tggttctcgg tataggcgac ctgcaccggg cggaactcga acaggttgat catcgtcgcc
     3121 ccgtgctgca cagcgccaaa aaacatccgc cgccaattgt ccggcgtgtt ccccggccag
     3181 tgcggcatta catagaacat gctctcgcga tctgtttttc cgcgattggc cgcccgggcc
     3241 agatcgagac tgatgttgtt catctgctgc gagccggcgg gaacctggaa gatgtagtcc
     3301 tcgctccagg gcatggtcat gccgtcctgt cggaagcagg tgacccactt gaagacctcg
     3361 cccaggtacg cgtgctcctg cccgccgtga tgcggcgaaa aattcgcccc gatcgccgca
     3421 ctgggcaggt gctgccgcag gatgtcggtc cgcttcttga tctcccggat gccgaagtcg
     3481 tattggtagc gcttcgacca gtaatacaat ccagggttgg ccgccttcat gttttcggcg
     3541 ggactatata cggctagttc cggcgtgagg ccgaggctgc cggctgtgat gccttgaccc
     3601 ttgagccact ctcgaaagac agccggatca gaggctcccg gcggctcagg caggccgatc
     3661 tcgtccccct ggctgacgac gcggatgtcc ttggcccgct cgccgagctg ctggcagtat
     3721 tcggccagtt tgtcggtcgc gacgccgcgg acgtcgatgt agccccgcgg accttccagc
     3781 cccggcagcc ggtcgtcttc ctgcccgcgc gcaccagcca ggctgaatag ccgccggaat
     3841 tcctcgaccg cggcggcgtg tttcgcgtcc ccctcaagcg gcgtgaacgt gtagccatag
     3901 atcggtgtcc gcaccggcgc cttcccgtgc atcggctgct gctttagatg agcgagcagt
     3961 tcgtacagca cgtcggcctg gtgaatcacg cggcgacgat agcgcgtgtc gccgaaatag
     4021 gccagcggca atcgctcgac tgtgccgtcg aatgtgccga tcgactcaat tgcgcccgct
     4081 gcgttcttca cgccgaattc gtagcgatac ttcagcccct cgccgccggc gaccagctca
     4141 agctgcccgt gattgagcga atcgatccag ctcccgatct cgacccagtc agtcgtctga
     4201 cccgccgtga ccatcagcgg gccgaacttg ccccaccgcc gctggtgaat ccagtaaggc
     4261 gaatgctcgg tcccgaggcc gccggtgttg ccccccgccg tgatcgtcag cggttgcgtg
     4321 ctttgattcg tcaccttgag ataaatatcg cccgactgcg tcagcatccc atccagcggc
     4381 agatagctct ccttctcgat tcgctcggcg acttcctcgg ctttggtcgt caacatcacc
     4441 aggtcgatgt tgcgtttggc cgccggcttg ggctgcttgt cggcgatcag gcggataatc
     4501 gcccgccccg gctgcagatc ggccgccgcg tcgtgccctt cccaaacgat gctctccccc
     4561 gcgccccagg accactgcag ctccgtcttg agcttctggc tgaaggccca gatcttcagg
     4621 ttgtcgcgag tcccgtacag ccgatcgagc gcgagtttgc cgccttgctc gacctgcacg
     4681 cgaaactgcg tctggaaccg gtaagccgct tcataccgca ccagcgtcag gtattttccc
     4741 gccgcgggaa tctcgacggc gatctcggcg accgtcttgt cacactgctc gggcgcgccc
     4801 agatacgcct gccggctgag aaacgtgttg gccagcgttg ccgcgtagta gttcgtcccg
     4861 aacgccttcg tctcccagcc gggcgcgcgg acctgaaact cctccccttc gcagacgatc
     4921 aggacatccg gctgcaccac caccgccggc cgcgacttgc caaacgcctg tgcagcagcg
     4981 ggcaggccgg cttcctctgc gcttgcgcaa gtcacgatta cgagcgaaac ggcgagggag
     5041 caaccaagag tgcgaatcat gggtgggatc cgatcgcggc gggaggcatg gaaggtgggc
     5101 cggctgccat tgtcggcagg accgcgcgcg aagtccaccc atcgcacaca acggcggcag
     5161 acaagaatgt ctgccccacg gcctcctacg tcaccgccat ctccggcgtc cgcaccgggg
     5221 cgccgacggc atcgtggccg acgacgcagt tccgcccttg gttgaccgca gcggccagcg
     5281 ccatgcggcc gcgctgcagg atctgccgca tgtcgtcgcc gtcgctcgcc tccaccacgc
     5341 cgatgctcac cgtgaaccag ctcgcgccgg ctttccgcgg caggcggcag cgctcgaccg
     5401 catgccgcag gcgctccgca attcccacgc tgtcggccag caaggccccg ggcagaatca
     5461 gggcgaacgt atcctcgccc aggcgggcca cgtggtccat gtcgcgcatc accgcgttga
     5521 tgagctgcgc cgtgacccgc agcaccacct ccgatgccga cggcccgtga tcgttcacaa
     5581 tccgcgggag cgaatcgacc tgcaccaaga gcagcgccag cggcgtccgc ccccgccgcc
     5641 actgcgacag ccgccggatc aaatcgtcga aaaacgccgg ccggctggag acttgcggaa
     5701 tcggctcgcg ggcgaggact tcggtcgatt cctccgattc gaacagccac tcgtcgccga
     5761 cgctgctggc cgcgatcggc accggcggca gaatccgcgc ctgctcgggc ttgatgaagt
     5821 gattggtccg cccgtcgtta tagtgagcac agttccgtcc ggccttcttc gacgagtaca
     5881 gcgcctcgtc ggctcgccgc acaatttctt tttcgtcgtc gccgggcaaa agatcggcca
     5941 gcccgctgct gagcgtgacg ctgagcgatt tgccgcccgt gcgaagctgc gttccggcca
     6001 ccgcctggcg ggcccgctcg gcctggccga cgatcgactc atagctcaga ccggaacaga
     6061 caaccgcgaa ctcctcgccg ccatagcggg ccaccagccc catatcgccc agcgagctgc
     6121 gcagcacccg cgccacgccg cgcagcactt cgtcgcccgt ctgatgcccg tgcgtgtcgt
     6181 tgaatcgctt gaagtgatcg acgtcgaaca gaatcagcgt cgccggcatc ccgcgccgct
     6241 gatgatcggc cgcgcaccgc cgcaattcgt cgtcaaaggc tcggcgattg gcaagctgcg
     6301 tcagcgcatc ggtgcgggcc tcggccgctt gcgattcgat ctgccgggcc tgcgcctgca
     6361 ggcgttcttc ggccgtatcg agctgccgct gcatccgctg gttggcttca atcaaatcgg
     6421 acacggcggc cagcacgctc tcgtcgtccg attccagcga ctggctgagc tgcgcctgga
     6481 tctcctcgac gcacgacgaa tgttgatcga cgtccgcctt gaccttccgc gtcagctcct
     6541 gcagccggct caatgtctcc tgcgcgaact gcgcctgctc gtccggctcc tcctcgctca
     6601 gcaccagata ccagcgcgcg gcgacccacg ccaccgccat gccggcaacg gctgccacga
     6661 gggtaatcag cacatacagc caggtcgtca taaccgccac aggccgcgcg tacggagagg
     6721 acgccactct actgaatggt ccattcaacc tcttacgttg cgcgcggggt cagtcaagct
     6781 cgtttaacgc gtagccgaag cgaagcggag gcgtagccgc ctttcgggtg gaggccgcaa
     6841 tcggctacgc ctgcggctac gcgttaaacg acattagcaa tgcagtcctc aatagcccaa
     6901 agatgccata aaccggatcg gtcgcgctaa tccacctgca agcgcagccg ggcctcatcg
     6961 gcccacggac tttccggcga aagctccagg aaccgcgacc agtgcgccgc cgcttcttcc
     7021 gggcgggaca gttcgtccaa cacgcgggcc aggtgatagt gagcgtccgg atacggctcg
     7081 tgcgattgca gcgtcccctc gaacgccgcc gcggccagct ccttctcatc cagctccagc
     7141 gccatgaaat accgctcgcg ggccgccgcc agatcgcccg cccggtacag cacctcggcc
     7201 agttgaaagc acagctccgg ctgcggcccg ccggcggcca gcgccgcgcg ataggtctcg
     7261 gcagcggccc gcaggtcgcc cgcttcgtcg aggtcggcgg cccaggcggc gagctgctcg
     7321 ggcgcggcat gtgccacatt caattcgccg ggtagggcag gggactgccg cgcgaggaag
     7381 atcgccgggc tggcgatcgt cgcgggcggc tcgtcggccg gatcgtcctc gtccagcgca
     7441 tcgaagtcga tccgcaactg gccgctcgtt tcgaccagcc ccgtcccgcg gcggacgagc
     7501 agcttcctgc cgtcgagcac cagcgatagc tcggccagcg gccgggccac atcgggaaag
     7561 tgccggtgaa tctcggccag cttccgcgcg atcagccgcg tgctcgcccc ggcccgcgtc
     7621 aactccgcga gctgccgggc gaccgacagc tcggtgaaat cgaacgttgc cagccggtgc
     7681 gtctcttcca ccggcacgag ccacccgcgg cgttgccacg cgcggacgcg agcggcgttc
     7741 acgccgacga gctcggctaa cagggcggcg gtgtaggggg cagacatggc agggaacagg
     7801 ggacagggaa caggggacag ggaacagtat tatccccgct tcggccaggt gagccagaag
     7861 atgctggcga aggcggcgag gctggcggcg acgcagacga tggcgcacac gatgaagatc
     7921 gtgttctccg tgccgctgcc caaagcctgg ccccggttgg cttccgccgc gaagcacacc
     7981 gccaatatcg acgccgtgca ggccgcaaag aacgctcccg ccgccgccgt cagtgcggcc
     8041 agcgcgatcc ccgccgaagc catgaacgag agcagctttt cagacacgtc catcgccttg
     8101 ccccgccgcc gcgcctgatg cccttcatac acggtccgca gcaaggccgg cgccgccacg
     8161 atcagcgcca gaacgcccag gcccggcgtc gctacgaatg cccccaggca cacggccgcc
     8221 agcgtgatca ccaagagcag cgactcgagc gaaaactgaa acggccgcgg ctcaacgccc
     8281 acgcgcggcg accagctcgc ggcaaatggc gattcgctcg cctcatcgac cacgtccgcc
     8341 gtcagcacca gctcgcgccc gcacagccag cacttggc
//
LOCUS       DASUGB010001110         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1653507, whole genome shotgun sequence.
ACCESSION   DASUGB010001110 DASUGB010000000
VERSION     DASUGB010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653507"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFV87_21110"
     CDS             complement(<1..368)
                     /locus_tag="VFV87_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106339.1"
                     /translation="MLLADNEVGGFLFFFVIIAIIVGFGFAIAAAQQQKQQQVLERVA
                     QRFRGRLEPGNLFQSGQIRLRFQNYPALVKYTKVGKNSYHTHFTITWPEHHLRCEVYP
                     QDILSGFRKLWGMEDIEIGSP"
     gene            complement(484..1362)
                     /locus_tag="VFV87_21115"
     CDS             complement(484..1362)
                     /locus_tag="VFV87_21115"
                     /inference="COORDINATES: protein motif:HMM:NF025802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HEX5106340.1"
                     /translation="MEVVVIFILLAGFILAAAIVVAAQSGSRASGWSQAFEHAARRFH
                     GKLMSGGWLSQPSAWLQHGQSSARLTVVKLPRSGGERYLQMTIQQPDFRSRCEIFYYQ
                     TRPELIQSMRGLAAVEFDWDEFRTRWHVLAADGDEVRHCLSDGVRLAIDQLWRLPVPG
                     EAAISLSPGWLVVRKMWGPPRGAELEEFVERVCALHDQLCLAAAAGIEFIASDQAQLL
                     EAARCGVCGDSLAAEIVVCKRCNTPHHRDCWDYGGTCATYGCGSRECFYPGLAPLAAP
                     HWSTRSLAEPRPAKPR"
     gene            complement(1530..2234)
                     /locus_tag="VFV87_21120"
     CDS             complement(1530..2234)
                     /locus_tag="VFV87_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HEX5106341.1"
                     /translation="MDQPSREARLKADHEALLALDAASSILSLEVSGDPPDRYTITFT
                     GKGLGRDASASSEITTIELHQIDLRMPYSYPNSPPDIRWITPILHPNVSFSGFVNLPD
                     IGIVWTKEVPIDMLLERLWDVARAEFMNLNKATNYSAKNWFEKECQQALPMDHRPLRD
                     QAARSGTNIVRYERRGAGQGVQFAGAASPSEVMFIDETTPTPQIPKRQPYVPVGRRRP
                     GSGGGDVMYIGPEGEG"
     gene            complement(2370..3770)
                     /locus_tag="VFV87_21125"
     CDS             complement(2370..3770)
                     /locus_tag="VFV87_21125"
                     /inference="COORDINATES: protein motif:HMM:NF013557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX5106342.1"
                     /translation="MDDGIQFGEVEEAAPNRALRPDQNRHFAVAAYGSPVDGELPVFV
                     DLDVLADMEDHAQSDTSVELGGVLLGGQYEDDEGRPFVVVSDSLRAQHYESTKGSFTF
                     THDTWSAISREREQFPEELQMVGWYHTHPDWGVFLSGMDMFICDNFFNKKLDVAYVID
                     PCRGDRGMFQWTGDPRQRVRRQGGYFVTASRFREAELEHYVGELSGNMPVTTTSQRSA
                     AGGYGAPVVHLHQPHAPPPPPWQGVAIAGALAMQFLLMALIAWKVLVPDTAAQEGKLA
                     AAFKDWQKAYESRQELEIAEERLRTQGATMDRVVNALKGPGDSFTELQANVDQVARLE
                     ADVAARDAQVRDLENTLDDVRTRLTSLESKSENEAQRLKRQIASLESDNKKLETSLKD
                     TKKELAKHVPPADEKAEGEKPTGLSIWWWVGGGALAALVLVAGVWWMGERMSKDEGAG
                     ESPEEVKDQGSEEAKR"
     gene            4056..4493
                     /locus_tag="VFV87_21130"
                     /pseudo
     CDS             4056..4493
                     /locus_tag="VFV87_21130"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4494..5714)
                     /locus_tag="VFV87_21135"
     CDS             complement(4494..5714)
                     /locus_tag="VFV87_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5106343.1"
                     /translation="MSSDVLFIDDSDRYSRLRLIGWWDQDKIRAAKILVVGAGAIGNE
                     VLKNLALLGVGHVYVIDLDEIEDSNLTRSVLFRHEDCGKSKAIAAAEEMRIINPDVTV
                     TPIHGNVITDLGLGLFRDVDAVIGALDNREARLWVNRSCWKVGTPWIDGGIQEISGVA
                     KVFVPPDGACYECAMTENDYRLINLRYSCPLLKREDLQAGKVPTAPTIASMIGGLQVQ
                     EALKLIHGLPVEDGTALVWNGQANNFYKTQYQRKEDCLSHDTYPDPIELPLTAAATAD
                     ELFAAAAKHFATPTRSASVGAGTPTRSVSEGIPHLTLSLDRDLVVSLDCACGEKRRVL
                     KPQQLVGAEDAKCPKCGQIAKPVLEHSIDAGTPLAKEKLSALGIPPYDIVRIGDDQQE
                     QVFLLAGDRAAVAA"
     gene            complement(5853..>6193)
                     /locus_tag="VFV87_21140"
     CDS             complement(5853..>6193)
                     /locus_tag="VFV87_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106344.1"
                     /translation="GQQPQYVVVRHFHPALEAQGNSASLTFTHETWAALTRETETNFP
                     NEWLLGWQHTHPGFGIFLSGYDLFIHKNFFAQPWQVALVVDPRKKEFGFFHWRNNEVR
                     DCGFICSQEV"
BASE COUNT         1066 a         1955 c         1934 g         1238 t
ORIGIN      
        1 ggcgagccga tctcgatgtc ctccatgccc cacagcttgc ggaagccgga caggatgtcc
       61 tgcggataga cctcgcaccg caggtggtgt tcgggccagg tgatcgtgaa gtgcgtgtgg
      121 tagctgttct tgccgacttt cgtgtatttg acgagcgccg ggtagttctg aaatcgcaac
      181 cggatttgcc cggattggaa gaggttgccc ggctccaggc ggccgcggaa gcgctgggcc
      241 acgcgctcca ggacctgctg ttgcttctgc tgctgggccg cggcgatggc aaagccgaaa
      301 ccaacgatga tcgcaatgat cacaaagaag aacaggaacc cgccgacctc gttgtccgcc
      361 aggagcacgt tgttgtccct cggttgtctg tagggtggga ccagctcgcg gagcgagcgc
      421 aggcccaccg cgctcttggt gggcctgcgg cctatcggcc ttgtcccacc ctacgatcta
      481 cgattaccgc ggcttggccg ggcgcggttc ggccagtgag cgcgtggacc agtgcggggc
      541 ggccagcggg gcgaggcccg gataaaagca ctcgcggctg ccgcagccgt aggtcgcgca
      601 cgttccgccg tagtcccagc agtcgcgatg atggggcgtg ttgcaacgct tgcagacgac
      661 gatctccgcg gccaggctgt cgccgcaaac gccgcagcgg gccgcctcca ggagctgcgc
      721 ctggtcgctg gcgatgaact caatgcccgc cgccgccgcc aggcatagct ggtcgtgcaa
      781 ggcgcagacg cgctcgacga actcctccaa ctccgcgccg cgcggcggtc cccacatctt
      841 ccgcaccacc agccagccgg gcgaaaggct gatggccgct tcgccgggca ccggcagccg
      901 ccagagctgg tcgatcgcca ggcggacgcc gtccgagagg cagtgccgca cttcgtcgcc
      961 gtcggcggcg agcacgtgcc aacgggtgcg gaactcgtcc cagtcgaact caaccgcggc
     1021 caggccgcgc atcgactgaa tcagctccgg ccgcgtctgg tagtagaaga tctcgcaccg
     1081 cgagcggaaa tcgggctgct ggatcgtcat ctgcagatag cgctcgccac cagatcgcgg
     1141 cagcttgacg accgtcagcc gggcggacga ttgtccgtgt tgcagccacg ccgaaggctg
     1201 gctgagccag ccgccgctca tcagcttacc gtgaaaccgc cgggcggcgt gctcgaaagc
     1261 ctggctccag ccgctggcgc gcgacccgga ctgggcggcg acgacaatcg ccgccgccag
     1321 gataaagcca gctagcagga tgaagatgac gacgacttcc acgggcgatc cttcagagaa
     1381 tcaatcgatt ctcactcgcg acggctgctg ctgcaacggt gagtgaccga gactttggag
     1441 tgcggcgact cgttaccgct tttggacctt tggatcgccg cagaattcct aaattctcaa
     1501 agcggggaca agtccccgca ctccaaaggt cagccttcgc cttccggccc gatatacatc
     1561 acatccccgc ctcccgaacc cggtcgcctc cggccgacgg gcacgtacgg ctgccgcttg
     1621 ggaatctgcg gcgtgggggt ggtttcgtcg atgaacatga cttcgctggg gctggcagcg
     1681 ccggcgaatt gcacgccttg gccggcgccg cggcgctcat agcgaacgat gttcgtcccg
     1741 ctgcgggccg cctggtcgcg cagcggtcgg tggtccatcg gcagggcttg ctggcactcc
     1801 ttctcgaacc agttcttggc cgaatagttc gtcgccttgt tcaggttcat gaactccgcc
     1861 cgggcgacat cccacagccg ctccagcagc atgtcgatcg gcacttcctt cgtccagaca
     1921 atgccgatat cgggcaggtt cacaaagccg ctgaacgaga cgttggggtg caggatcggc
     1981 gtgatccagc ggatgtcggg cgggctgttc ggatacgaat agggcatccg caggtcgatc
     2041 tggtgcagct cgatcgtcgt gatttcggac gacgccgacg catcgcgccc cagccctttg
     2101 cccgtgaacg taatcgtata gcggtcgggc ggatcgccgc tgacctccag gctgaggatt
     2161 gagctggcgg catcgagcgc caggagcgct tcgtggtccg ccttcaagcg ggcttcgcgg
     2221 gatggttggt ccacggctag ctccggggtt cggggttcgg ggttcggggt tcggaggcca
     2281 gaggtcagag gtcgaaatca cttggaagct ttgtactcag tactcagtac tcagtactca
     2341 gtacagccca atctttcacc cttcatgttt taccgctttg cttcttctga gccctggtct
     2401 ttcacttctt ccggcgattc gccggctcct tcgtctttgc tcatccgctc gcccatccac
     2461 cagacgccgg cgacgaggac gagggcggcc aaggcgccgc cgccgaccca ccaccagatc
     2521 gaaaggccgg tgggcttttc gccctccgct ttttcgtcgg ctggcggcac atgcttggcc
     2581 agctcttttt tcgtgtcctt gaggctggtc tccagtttct tgttatcgga ctccaggctc
     2641 gcgatctgtc gcttcagccg ctgggcttcg ttttcggatt tcgattccag ggaggtgagg
     2701 cgcgtgcgaa catcgtccag cgtgttttcc aggtcgcgga cctgcgcgtc gcgggcggcg
     2761 acgtcggcct ccaggcgggc gacctgatcg acgttggcct gcagctcggt gaagctgtcg
     2821 cccggtccct tcagagcgtt cacgacgcgg tccatcgtcg cgccttgcgt ccgaagccgc
     2881 tcttcggcga tctccagctc ttgccgcgat tcgtaagcct tctgccaatc cttgaatgcc
     2941 gcggcaagct tgccctcctg ggcagccgtg tccggtacca gcactttcca ggcgatcagg
     3001 gccatcagca gaaactgcat cgccagtgcc ccggcgatcg cgacgccctg ccagggcggc
     3061 ggcggcggag cgtgcggctg atgcaaatga acgaccgggg cgccgtagcc gccggcggcg
     3121 gaacgctgag aggtggtggt gactggcatg tttccgctaa gctcccctac gtagtgttcg
     3181 agttccgcct cgcgaaatcg gctcgctgtg acaaaatatc cgccttggcg tctcactctt
     3241 tggcgggggt cgcccgtcca ttggaacatc ccccggtcgc cgcggcaggg gtcgatgaca
     3301 taggcgacat ccagcttctt gttgaagaag ttgtcgcaga taaacatgtc catccccgac
     3361 aggaacacgc cccagtccgg atgcgtgtgg taccagccga ccatctgcag ctcctcgggg
     3421 aactgctcgc gctcgcggct gatggccgac caggtgtcgt gcgtgaaggt gaacgagccc
     3481 ttggtgcttt catagtgctg ggcccgcagg ctgtcgctga cgacgacgaa cggtcgcccc
     3541 tcgtcgtcct catattgccc gccgagcagc acgcccccca gctcgaccga cgtgtccgac
     3601 tgggcgtggt cctccatgtc ggccagcaca tccagatcga caaacaccgg cagctcgccg
     3661 tccaccggcg agccatacgc cgccacggcg aaatgccggt tctgatccgg ccgcagcgcc
     3721 cggttcgggg cggcttcttc gacttcgcca aattgaatgc cgtcgtccac gattcttgtt
     3781 cttagccggg accaaacctg gtcccggagt cagcaattgc gttcgcgcat gccaagctgg
     3841 aagatacgcg acaatggaag tgctgttgtc accgcatatc gtagttcgtt ccgatgcaat
     3901 actcgatggc aaagaatctc ccacttcgac aaatcatggc atcgcgctgg cgtgccgcgt
     3961 cgtccggacc atatttcaag ctgaacgagc ctccgccatc gattcgtgat gcgctcccag
     4021 cggactagct cagcgtagtc cgcgagtttg gaggcaagga gggattcctc ggaaacaaat
     4081 accttcactt gtatcaatta gacgaattaa gcgatttcaa ttcttcctac gcagcaacaa
     4141 agtcgttccc ggaaggaatc attttcggtt cggacggttg cggggaggcg tacgcgtttc
     4201 gattgagtga cgttgcggtg gttcgggtgc cgttcattcc attgattgca gaatttgcta
     4261 cccacatcgc tccgacgttt tccgaattca tccagcattg cgcggacacc ggcgattcgg
     4321 gggatctcaa tccagatgtg attggaatgg aggtccacga caagcacccc attgcactcg
     4381 gcggcgatcc gactgccgag gacaacaaag tgtttgttcc gcccattcaa catgccgaac
     4441 tttgtcgcta ttggacggag gtgtattgcc gaattcgttc acaacaggaa tagctaggcc
     4501 gcgaccgccg cccgatctcc cgccagcaaa aacacctgct cctgctggtc gtcgccgatg
     4561 cggacgatgt cgtagggggg gatgccgagg gcggagagtt tttccttggc cagcggcgtg
     4621 ccggcgtcga tcgagtgttc gagaacgggc ttggcgatct ggccgcactt ggggcacttc
     4681 gcgtcttcgg cgccgacgag ctgctgcggc ttcaggacgc ggcgcttctc gccgcaggcg
     4741 cagtcgagcg agacgaccag gtcgcggtcg agggagaggg tgaggtgggg gattccctcg
     4801 cttacgcttc gggttggtgt gcccgccccc acgcttgcgc ttcgggttgg tgtggcgaag
     4861 tgcttcgcgg cggcggcgaa cagctcgtcg gcggtggcgg cagcggtcag cggcagctcg
     4921 atgggatcgg gataggtgtc gtggctcagg caatcttcct tccgctggta ctgcgtcttg
     4981 taaaagttgt tggcctggcc gttccagacg agggccgtgc cgtcttcgac cggcaggccg
     5041 tggatcagct tgagggcctc ctgcacctgc aggccgccga tcatgctggc gatcgtcgga
     5101 gcggtcggca ctttgcccgc ctgcaggtcc tcgcgcttga ggagcgggca gctataccgc
     5161 aggttaatca gccgatagtc gttttcggtc attgcacatt cgtagcacgc gccgtcgggc
     5221 ggcacgaaga ccttggccac gccgctgatt tcctgaatgc cgccgtcgat ccagggcgtg
     5281 ccgaccttcc agcaggagcg gttgacccac aggcgggcct cgcggttgtc gagcgccccg
     5341 atcaccgcgt ccacatcgcg aaacagtccc aaccccagat cggtgatcac gttgccgtgg
     5401 atcggcgtca ccgtcacgtc cgggttgata atccgcatct cttccgccgc ggcgatcgcc
     5461 ttacttttgc cgcagtcttc atggcggaaa aggaccgatc gcgtcaggtt gctgtcctcg
     5521 atctcgtcca ggtcgatcac atacacgtgc cccacgccca agagtgccag gttcttcaac
     5581 acctcattcc caatcgcccc cgcgccgacc acgaggatct tcgccgcccg aatcttgtcc
     5641 tgatcccacc agccgatcag gcgcagccgc gagtagcgat cggaatcgtc gatgaagagt
     5701 acgtcactcg acatggtttg tccctttcag ccaccgctgc ggcggcgggt cttgaacaga
     5761 aggtcgcaaa gagcgcaaag aagacaattg aagttctgct tctttgcggc ctttgcgatc
     5821 ttctgttcca tttccgattg cccgggaccg gatcaaactt cctgcgaaca tataaaaccg
     5881 cagtcccgca cttcgttgtt ccgccaatgg aaaaacccaa actccttctt ccgcggatcg
     5941 accacgaggg cgacttgcca cggctgggcg aagaagtttt tgtggatgaa caggtcgtag
     6001 ccggacagga aaatgccgaa gccggggtgc gtgtgctgcc agccaaggag ccattcgttg
     6061 gggaagttgg tttcggtctc gcgggtgagg gcggcccacg tttcgtgcgt gaacgtgagc
     6121 gaagccgagt tcccctgggc ttccagcgcc gggtgaaagt gccgcaccac cacatactgc
     6181 ggctgctggc cat
//
LOCUS       DASUGB010001111         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_541490, whole genome shotgun sequence.
ACCESSION   DASUGB010001111 DASUGB010000000
VERSION     DASUGB010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541490"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VFV87_21145"
     CDS             <1..1214
                     /locus_tag="VFV87_21145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5106345.1"
                     /translation="DTLGQIERNYVQPVDRRELMEAAIRGMLSKLDPHSSYIPPAELD
                     RFKSNVENEFGGIGVTVTLESGELTVVSPMLAAPAYRAGIRGGDLFTEIDGQATAGWS
                     IDEAVRRVRGKIGTSVKVTVKHAADGKTETVEVPRELVRVDSVLGDRRKADDTWNFFL
                     DEEKKIGYVRISSFSRHTPDELRTALGELQRGGLAGLVLDLRFNPGGLLTAAIEVCDL
                     LVPSGRIVSTEGRSGPHRVWDAHQEGTFEGFPIAVLINHTSASASEIVAACLQDHGRA
                     TIIGERTWGKGSVQNIVELEEGKSALKLTTAGYMRPSGKNIHRREGAAEKDEWGVRPD
                     AGFELALSGDENDAYQADRRKRDAIVAHSPDAAPPAGEPKYDKQLQMAVDHLLKKIAD
                     AAAPLETADAK"
     gene            1312..2334
                     /gene="tsaD"
                     /locus_tag="VFV87_21150"
     CDS             1312..2334
                     /gene="tsaD"
                     /locus_tag="VFV87_21150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5106346.1"
                     /translation="MILLTIETTCDETAAAVVTEELAVLSSVVASQDELHRRFAGVVP
                     EIAARAHVERILPVIDEALRRAGVALGDLDAIAVANTPGLAGSLLVGLVAAKTLCVAA
                     DKPLVAINHLHAHIYACRMAAGREVFPCLGLIVSGGHTSLYHCAGPVDFEYLGGTIDD
                     AAGEAFDKVAAMLGLAYPGGPSISRAAESGNPRAYHFPRGLAGDKRRLDFSFSGLKTA
                     VRYAIAGPGNPDISQARLTPQDIADLAASFQAAVVESLVEKTLLALARMQLETLCVGG
                     GVAANRLLRERLADAARERGFELHIAPPELCTDNAVMGAIAFERLKAGHTEPLDLDVI
                     PGLVRR"
     gene            complement(2353..>2819)
                     /locus_tag="VFV87_21155"
     CDS             complement(2353..>2819)
                     /locus_tag="VFV87_21155"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5106347.1"
                     /translation="GQSAFKAPDLVGSGENWLQSQPLAPDELRGKVAVVHFFAFGCIN
                     CIHNYPSYRNWQTDLAGKDVVLVGIHTPETTAERSVETLKSKLKAESLAFPVLVDNEQ
                     ANWNAWGNNVWPAVYLIDKQGYFRSYWPGELNWQGREGEAYMRKQIDALLAE"
BASE COUNT          497 a          870 c          964 g          488 t
ORIGIN      
        1 ccgacacgct cggccagatc gagcggaatt atgtccagcc ggtcgatcgc cgcgaactga
       61 tggaagcggc gattcgcggg atgctttcga agctcgatcc gcattcgagt tacattccgc
      121 cggcggagct ggaccgcttc aagagcaatg tcgagaacga attcggcggg atcggcgtga
      181 cggtgacgct ggagtcgggc gagctgacgg tcgtttcgcc catgctcgcg gcgcccgcct
      241 atcgggccgg catccgcggc ggcgatctct tcaccgagat cgacggccag gcgaccgccg
      301 gctggtcaat cgacgaagcg gtgcgccgcg tgcggggaaa aatcggcacg tcggtcaaag
      361 tcacggtcaa gcacgcggcg gacggcaaga ccgagacggt cgaagtgccg cgggagctgg
      421 tccgcgtcga ttcggtcctc ggcgaccgcc gcaaagccga cgacacgtgg aatttcttcc
      481 tcgacgaaga gaaaaagatc ggctacgtgc gaatctccag cttcagccgt catacgcccg
      541 acgagctgcg gacggccttg ggcgaattgc agcgcggcgg cctggcgggg ctggttctgg
      601 atttgcggtt caacccggga ggcctgttaa ccgcggccat cgaggtgtgc gacctgctcg
      661 tgcccagcgg ccgaatcgtc agcaccgagg ggcggagcgg gccgcaccgc gtgtgggacg
      721 cccaccagga agggacgttc gagggattcc cgatcgcggt tctcatcaac catacgagcg
      781 ccagcgcgag cgaaattgtc gctgcctgcc tgcaggatca tgggcgggcg acgatcatcg
      841 gcgagcggac atggggcaaa ggaagcgtgc agaacatcgt cgagctcgaa gagggaaaga
      901 gcgctctgaa gctgacgacc gccggctaca tgcgtcccag cggcaagaac atccatcgcc
      961 gtgagggggc cgccgagaaa gatgagtggg gcgtgcggcc ggatgcgggc ttcgagctgg
     1021 cgctatcggg cgacgagaac gatgcctatc aggccgatcg ccgcaagcgg gacgcgattg
     1081 tcgctcattc gcccgatgcc gccccgcccg ccggcgagcc gaagtacgac aaacagcttc
     1141 agatggccgt cgatcacctg ctcaagaaga tcgcggatgc cgccgcgccc ctcgaaacgg
     1201 cggatgcgaa gtagcgcgga gaaagaacca agaaccgaag aaccgaagaa ccaaaggttt
     1261 tgagatctct gtttggttct tggttcttcg gttcttggtt ctttgatcct catgatcctg
     1321 ctgaccattg agacgacctg cgacgagacg gcagccgcgg tcgtgaccga ggagctggcc
     1381 gtgctgtcgt cggtggtggc ttctcaggat gaattgcacc ggcggttcgc gggcgtggtg
     1441 ccggagatcg ccgcgcgggc gcatgtcgag cggattctgc ctgtgatcga cgaggcgctg
     1501 cggcgtgccg gtgtcgctct gggtgatctg gatgcgatcg ccgtggcaaa tacgcccggt
     1561 ctggccggct cgctgcttgt cggcctcgtc gcggcaaaga cgctgtgcgt agccgcggac
     1621 aagccgctgg tggcgatcaa ccacctgcac gcccacattt acgcctgccg gatggcggcg
     1681 gggcgcgagg tttttccgtg cctcggcctg attgtgagcg gcggacatac gagtttgtat
     1741 cactgcgccg ggccggtcga tttcgagtat ctcggcggca cgatcgacga cgcggccggc
     1801 gaggcgttcg acaaggtcgc ggcgatgctc ggcctggcct atcccggcgg gccatcgatt
     1861 tctcgagccg ccgagagcgg caatccgcgg gcgtaccact tcccccgcgg actggccggc
     1921 gacaagcggc ggctggattt cagcttcagc ggattgaaaa ccgcggtgcg ctatgcgatc
     1981 gccgggccgg gcaatcccga tatttcccag gcgcgattga cgccgcagga catcgccgac
     2041 ttggccgcca gctttcaagc cgcggtcgtg gagagcctgg tggagaagac actgctggcg
     2101 ctggcgcgca tgcagctcga gacgctgtgc gtcggcggcg gcgtggccgc caatcgcctg
     2161 ctacgcgagc gtctcgcgga cgctgcacgg gagcgcggct tcgagctgca catcgcaccg
     2221 cccgagctct gcaccgacaa cgccgtcatg ggcgccattg ccttcgaacg cctcaaagcc
     2281 ggccacaccg agccgctcga cctcgacgtg atccccggtc tggtgcggcg ttaggccgta
     2341 accagcggca gactattccg cgagcagcgc gtcgatttgt ttgcgcatgt acgcttcgcc
     2401 ttcgcggcct tgccagttga gctcgccggg ccagtaggag cggaaatagc cctgcttgtc
     2461 gatcagataa acggcgggcc agacgttgtt gccccaggca ttccaattgg cctgctcgtt
     2521 atcgaccagc accggaaacg cgagcgactc ggccttcagc ttgctcttca gcgtttccac
     2581 gctccgttcg gccgtcgttt cgggcgtgtg gatgccgacg agcacgacgt ccttcccggc
     2641 cagatccgtt tgccagttgc gatacgacgg atagttgtga atgcagttga tgcagccgaa
     2701 ggcgaagaaa tggacgacgg cgaccttgcc gcgaagctcg tctggcgcga gcggctgcga
     2761 ctggagccag ttttccccgc tgccgaccag gtcgggcgcc ttgaaggcgg actggccca
//
LOCUS       DASUGB010001112         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1374327, whole genome shotgun sequence.
ACCESSION   DASUGB010001112 DASUGB010000000
VERSION     DASUGB010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374327"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /locus_tag="VFV87_21160"
     CDS             complement(<1..1893)
                     /locus_tag="VFV87_21160"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5106348.1"
                     /translation="MSQLGFEPILGSYLAVALLALGLVALLLIGPTFGALSRPRRMTL
                     LVLRLIVIFLAILALLRPTWVTTVRTPRPSVLLLLADVSRSMQLPSGRSEQSRWRAQA
                     NALAESSRELARLAGRNQTEVRAYAYDYKLRPLEISGGKLSLPESPTGEQTDIGTTLH
                     DALRAEQGKRLMAVFVLGDGSQNAFDPLVETQDAARKLRDDFAAPLFAVTFGLSGSEA
                     QARDVAVERLDEQFTVFVKNELVVRAILRVRGYVKQDLPVELLLTDDKGQSQPIGRRT
                     IRADEDNQQVEVEFLYTPQTPGHFKLTVAADPQAGELVTKNNRLDAYLTVLEGGLRVL
                     YLDGEKRFEQKFLRRAINASPDIELDDRIIDRRSEDKWPVDLGRDFAENKYDAFILGD
                     LDAAALGPENLKVLADAVGTKRKGLLMIGGRTSFGRGHYLNTLVGDALPITIDRLEGQ
                     EFANEENVDKFFLPGPLPLVPAAQHAITRLAPDAENVAVWSKLPPLNWANKFYGLKET
                     MPGLRVLLETPQKQPLLVSGEYGKGRTLAFAGESTYLWPLHGFEKEHKRFWRQVILWL
                     VRRDDLNRDDVWIKLDQRRLSPGTRAIVEAGARTGAGDPIKNARMETFLIRPGNKKEK
                     FDLSQDD"
BASE COUNT          349 a          681 c          730 g          384 t
ORIGIN      
        1 gtcgtcctgc gaaaggtcga atttctcctt tttgttgccc ggacgaatga ggaacgtttc
       61 catccgggcg ttcttgatcg gatcgcccgc gccggtgcga gcgccggctt cgacaatggc
      121 ccgcgtgccg ggactcagcc gccgctggtc gagcttgatc cacacgtcgt cgcggttcag
      181 gtcgtcgcgc cgcaccagcc acaaaatcac ctgccgccag aaccgcttgt gttccttctc
      241 gaagccgtgc agcggccaga gataggtcga ttcgccggcg aaggccagtg tccggccttt
      301 gccgtattca ccgctcacga ggagcggctg cttttgcggc gtttccagca gcacgcgcag
      361 gcccggcatc gtttccttga ggccgtagaa cttgttcgcc cagttgagcg gcggcagctt
      421 gctccagacg gccacgttct cggcatccgg cgcgaggcgc gtgatcgcgt gctgggccgc
      481 gggaaccagc ggcagtgggc cggggaggaa gaacttgtcg acgttctcct cgttggcgaa
      541 ttcctggcct tcgaggcggt cgatcgtaat tggcaacgcg tcgccgacca gcgtattcag
      601 ataatgcccg cggccgaagc tcgtccgccc gccgatcatc aagaggccct tcctcttcgt
      661 gccgactgcg tcggccagga ctttcaggtt ctccggcccc agggccgccg cgtcgagatc
      721 gcccaggatg aatgcatcgt acttgttctc cgcgaaatcg cggcccagat caaccggcca
      781 cttgtcctcg ctgcggcggt cgatgatccg gtcgtccagc tcgatatcgg gcgaggcgtt
      841 gatcgcccgc cgcaggaatt tttgctcgaa ccgcttctcg ccgtcgagat acagcacccg
      901 caggccccct tcgagcaccg taaggtaggc gtccagacgg ttgttcttcg tgaccagctc
      961 gcccgcctgc gggtcggcgg cgaccgtcag tttgaaatgc ccgggcgtct gcggcgtgta
     1021 gaggaactcg acttcgacct gctggttgtc ttcatcggcc cggatcgtgc ggcggccgat
     1081 cggctgcgac tgccctttgt cgtcggtcag caaaagctcg accggcaggt cctgcttcac
     1141 gtagccgcgg acgcgaagga tcgcccgcac gacaagctcg tttttgacga acacggtgaa
     1201 ctgctcgtcc agccgctcga ccgccacgtc gcgggcctgg gcttcagagc cggagaggcc
     1261 gaaggtgacg gcgaagagcg gggcggcgaa gtcgtcgcgg agcttgcggg cggcgtcctg
     1321 cgtttcgacg agcgggtcga aggcgttctg cgagccgtcg cccaagacaa acaccgccat
     1381 cagccgcttg ccttgctcgg cccgcagggc gtcgtgcagc gtggtgccga tgtcggtctg
     1441 ctcgccggtg ggtgattccg gaagggagag cttgccgccg gagatttcca gcggcctgag
     1501 cttgtagtcg taggcatagg cgcggacttc ggtctggttc cgcccggcca ggcgggccag
     1561 ctcgcgggac gattcggcca aggcattggc ctgagcccgc cagcgcgatt gctcgctgcg
     1621 gccgctggga agctgcatgc tgcggctgac gtcggccagg agcagcagga cgctggggcg
     1681 cggcgtgcgg acggtcgtga cccacgtcgg ccgcagcagg gccaggatcg ccaggaagat
     1741 gacgatcagc cgcaggacca gcagcgtcat cctccgcggc cgcgagaggg cgccgaacgt
     1801 cggcccgatc aacagcaacg ccacgagccc aagcgccaga agcgccacgg ccaggtagct
     1861 gccgaggatc ggttcgaagc cgagctggga catgccgagg gtcaggggac aggggtcagg
     1921 ggacaggggt caggagtcgg gggacaccag gctctgtttg aaatccacat gtgcggttca
     1981 gggtggccgg gtttttgcga agcaataacc cgggtggctg gggtagagct atcgctcaac
     2041 ctcaggcacc ccaacgtctt catctgccat cctccaagtg taccgcggcg gggaagtagc
     2101 ggcggagggt gcatcaaagc cgcgaccatt ggtcgcggtt gcca
//
LOCUS       DASUGB010001113         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1234910, whole genome shotgun sequence.
ACCESSION   DASUGB010001113 DASUGB010000000
VERSION     DASUGB010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234910"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFV87_21165"
     CDS             complement(<1..879)
                     /locus_tag="VFV87_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX5106349.1"
                     /translation="MTEQPPRFLNRELSWLEFNQRVLDEARDESLPLLERLKFLAITA
                     SNLDEFFMVRVGGLRVLVEQGITNPDPAGLSPAEQLAGISQRTHQMVQQQQACFADLE
                     AKLAEAGIRRVRGQDLTDRQARSLMQVFESEIFSVLTPMAATSAEEFPLLVNQMSQVC
                     VQLKPDPTLGDQPRFAIIPLGRSLPRYFTLGAERGYSFVLLEDVVQRMVDRFFPGEEV
                     TGHAVFRITRNADFSLRDDLATDLLAGMEGILTARKHGQCVRLEIDAAAGEGIVSFLT
                     KALDVGPDDLFPTSGPI"
     gene            1014..1262
                     /locus_tag="VFV87_21170"
     CDS             1014..1262
                     /locus_tag="VFV87_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106350.1"
                     /translation="MKRSPGVYIATILTAAPLLLVTVLAIYIAGYFLLADSKGLYRGR
                     WQFVLYEPAFRVQRWATGEPVNTGYVEPNGLHRNLRLP"
     gene            complement(1259..1960)
                     /locus_tag="VFV87_21175"
     CDS             complement(1259..1960)
                     /locus_tag="VFV87_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106351.1"
                     /translation="MLLHDHFRPPLSLRRHWHSFHSAWCTYLASHLNEQLPEGYFAEP
                     NVQFGIEIDVAAFEEGVSPSLAAGGWVAPAPMQSVAITLITDLAEVLIYGREGGPILA
                     GAIELVSPANKDRDAHRDAFVSKCAAYVQQGVGLAMVDVVTDRRANLHEELLARLQAE
                     QELPLDADLYATAYRPIEHDRNPSLDIWQHALAVGKPLPVLPLWLRGNLCLRVDLAAT
                     YDRTCREQRIPAEGA"
     gene            complement(2023..>3324)
                     /locus_tag="VFV87_21180"
     CDS             complement(2023..>3324)
                     /locus_tag="VFV87_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX5106352.1"
                     /translation="VRVVATSAVREASNRLAFIDRVYIATGFQIDPIEEAEVIRITFL
                     GVQPFLISDPSLAQSRCIVTEVGGGSTEVLMVKAGDVGYSHTYRLGSLRLRETLEALR
                     APTLKVRNIMETHIHRTVEEIAQHVSRDGPIELIAMGGDVRFAVRQLQPEWDGHSLAR
                     LPLKALEDLTNKILSQSEDKLVRKYRLSYPEAETLGPALLAYVLLAKELQLATLLVTN
                     VNLRDGLLMEMASSDAWSKEFTGQIIRSAMDLGRKFGYDEPHATHVSQLSSMLFHQLR
                     DEHQLPARYEVILQVAALLHEIGLFVSNRSYHKHSLYLIRNSELFGLGRKDLLLAALV
                     ARYHRRASPQPTHEGYATLDRDERVAISKMAAILRTADALDESRSQRIHEFTAQREGD
                     RLVISIPLVEDLSLETLALKQTGSLFEETFGLPVLLRMARG"
BASE COUNT          664 a          991 c         1032 g          637 t
ORIGIN      
        1 gatcgggccg ctggtcggga agaggtcgtc ggggccgacg tcgagagctt ttgtgagaaa
       61 cgacacaatt ccttcgccgg ctgccgcgtc gatttccagc cgcacacact gtccgtgctt
      121 gcgggcggtc aggattccct ccatgccagc cagcaggtcc gtcgcgaggt cgtcgcggag
      181 cgaaaagtct gcattgcgag taatccgaaa gacggcgtgg ccggttactt cttcaccagg
      241 gaagaagcgg tccaccattc gctgcaccac atcctccagc aggacgaatg aataaccgcg
      301 ctcggccccg agcgtaaagt accgcggcag cgagcgcccg agaggaatga ttgcaaagcg
      361 gggctgatcg ccgagagtcg gatcgggctt gagctgcacg cacacctgcg acatctgatt
      421 gaccaggagc gggaattcct ccgccgacgt cgccgccatc ggcgtaagga cggagaaaat
      481 ctcgctctca aatacttgca tgagcgatcg cgcctgtcgg tcggtcaagt cctgtccgcg
      541 cacgcggcgg attcctgctt ctgcgagttt tgcctccaga tcggcaaaac aggcctgctg
      601 ctgttggacc atctggtggg tgcgctggct gatgccggcg agttgttccg cgggcgacag
      661 gccggcagga tcgggattcg tgatgccctg ctcgacgagc actcgcagcc cgcccacgcg
      721 gaccatgaag aactcgtcca aattgctcgc cgtgatcgcc agaaacttca gccgttctaa
      781 gagcggcaaa ctttcatcgc gcgcctcatc gagcacgcgc tggttgaatt ccagccagga
      841 aagctcgcgg ttcagaaagc gtggcggttg ttcggtcatg gcgttaattc ggaaggaaga
      901 tggccgaaca agtacgggct gcgcactcga gacaatttac aacagaaagt gcgactcgtg
      961 gatcagccga agttggcatt ggttaccggg aggcgatgga atagcattca gatatgaaga
     1021 gatcgccggg agtgtatatc gcaacgattc tcaccgccgc gccattgctc ctggtcacgg
     1081 ttttggcgat ctatatcgcc gggtattttc tgctcgcaga cagcaaggga ctctatcgcg
     1141 ggcgatggca gttcgtcctt tacgaacctg cgtttcgagt gcagcgctgg gctacgggcg
     1201 aacctgtaaa caccggctac gtggaaccca atgggttaca tcgcaacctg aggttgcctt
     1261 aagccccttc ggccggaatc ctctgctcgc ggcaggtgcg atcgtaggtc gccgccagat
     1321 cgacgcgcag gcacaagttt ccgcgcagcc acagcggcag aacgggcagc ggcttgccga
     1381 cggcgagggc gtgctgccag atatccagac tcggattgcg gtcgtgctcg atgggccgat
     1441 acgcggtcgc gtataggtcc gcgtccagcg gaagctcctg ctcggcttgc aatcgcgcca
     1501 agagttcctc gtgcaggttc gcccgccggt cggtaacgac atcgaccatc gccagtccga
     1561 cgccttgctg cacgtaggcg gcgcatttcg agacaaaggc gtcgcggtgc gcatcccggt
     1621 ccttgttcgc gggactgacg agttcaatag cgccggccag tatcggcccg ccttcacgcc
     1681 cgtaaattaa gacttctgcc aggtccgtga tcagcgtgat ggcgacagat tgcatgggcg
     1741 cgggagcgac ccaaccgccg gctgccagcg aaggcgacac accctcctca aatgccgcca
     1801 catcgatctc aatgccgaat tgcacattgg gctcagcgaa atagccctcg ggcagttgtt
     1861 cattgagatg cgacgccagg taggtacacc aagcgctatg gaacgaatgc caatggcggc
     1921 gcaggctgag cggcgggcgg aagtggtcgt gaaggagcat gaagtcattc tacctgactt
     1981 gccaggagcc ttgccaatct cgctcaaggc gagcactccc gctcaacccc gcgccatccg
     2041 caacagcacg ggcaggccga acgtctcttc gaacagggag ccggtttgct tgagggccag
     2101 cgtctcgagc gacaggtcct caactagcgg aatcgagatc acgagccggt cgccttcgcg
     2161 ttgggccgta aattcatgga tccgctggct gcgcgattcg tcgagcgcat cggccgtgcg
     2221 aaggatggcg gccatttttg agatcgccac gcgctcgtcg cggtcgagcg tggcatagcc
     2281 ttcgtgcgtc ggctgcggcg aggcccggcg gtgatagcgg gccacgagag ccgccaagag
     2341 cagatccttt cgccccaagc cgaacagttc gctgttgcgg atcagataca gcgagtgctt
     2401 gtgatagctg cggttgctca cgaagaggcc aatttcgtgc agcagcgccg ccacctgcag
     2461 gatcacctcg tagcgggcag gcagctgatg ctcgtcgcgg agctgatgaa agagcatcga
     2521 ggaaagctgc gatacgtgcg ttgcgtgcgg ctcgtcgtag ccgaacttgc gacccaaatc
     2581 catggccgag cgaatgattt gccccgtgaa ttccttcgac caggcatcgc tgctggccat
     2641 ttccatcaag agaccgtcgc gaagattgac attggtgacc agcagcgtcg caagctgcag
     2701 ttctttggcg agcagcacat acgccaagag cgccggcccg agggtctctg cctcgggata
     2761 gctcagacga tatttgcgga ccagcttgtc ctcggattga gagaggatct tgttggtcag
     2821 gtcttccagg gccttgagcg gcaatcgagc gaggctgtgc ccgtcccact cgggctgcaa
     2881 ttgccggacc gcaaaccgca cgtcgccgcc catcgcaatt agctcaatcg gtccgtcgcg
     2941 gctgacgtgc tgggcgatct cctcgacggt gcgatggatg tgcgtctcca tgatgttccg
     3001 cactttgagc gttggggccc gcaatgcttc cagtgtttct cgcagccgga gactgcccag
     3061 ccgatacgtg tgcgaatagc cgacgtcgcc tgccttgacc atcagcacct cggtgctacc
     3121 tccgccaact tcggtgacaa tacaacgcga ctgagccagt gacggatccg aaatcaagaa
     3181 tggctgtacg ccgaggaagg tgatgcggat gacctccgct tcttcgatcg gatcgatctg
     3241 aaagccggtt gcgatataga cccgatcgat aaacgccagc cgattgctgg cttcgcggac
     3301 ggcgctcgta gcgacaacgc gaac
//
LOCUS       DASUGB010001114         4828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_401978, whole genome shotgun sequence.
ACCESSION   DASUGB010001114 DASUGB010000000
VERSION     DASUGB010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401978"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFV87_21185"
     CDS             complement(<1..307)
                     /locus_tag="VFV87_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106353.1"
                     /translation="MIRTSLAFAVVLIFTLAFLPPLAHAQEAKKEKLVTLCWFPDFSP
                     DGKWLVTPHGSWKTTEAGEVRVWNVVTGEAKHIIPSPRGVRTVLWSPKGTFFASGNYR
                     "
     gene            complement(539..1873)
                     /locus_tag="VFV87_21190"
     CDS             complement(539..1873)
                     /locus_tag="VFV87_21190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5106354.1"
                     /translation="MTAAIVLSPRASTPLAAQQPSLAPQQSHRLPPALPPPERLPPPV
                     LQVDPQAPSPPEELARAEIRLSDLEQIALQNHPQLLATVNAVAAAEGKAIQARLYPNP
                     QVGVAAPQIAGRESQYNAFASQEILTGGKLQLDSAAAWQEVEQSRLTMTRVRYDVLTG
                     VRRRFYAVLAAQNRVAVLEDLVSLATKSQKIGQMLFNAGEGTKTDTLLLEIELHKDQV
                     ALENARTVYEAGKRQLAAAVGVPQLAVGVLYGDLATELPRFDLPELQQRVIAVNPQTA
                     VAEAEARRARILLDRAYVEPRPTFNIMGGYQHQEQGVQDQAIAQVLMSVPLWNKNQGG
                     IRAARGTVGRAEADVRRVQVELAGQAAEALGRYRAALNTVERYQAQILPKSRESVSLT
                     QGLYQQGQIDFLRLLQAQRTLGEVNLGYIDAQESRWNAAADIANLLQVERFP"
     gene            complement(2006..4576)
                     /locus_tag="VFV87_21195"
     CDS             complement(2006..4576)
                     /locus_tag="VFV87_21195"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX5106355.1"
                     /translation="MQSLRLSLVVCLLLACSSAAAQQRSGPFNQPPRSLRSRTIDQKH
                     VRLELTFDFDKQEFRGRAIHQLALFQPAREIALDAAEMKIESVTLSPLPATSEQAPVE
                     PKFSQRDQSLTIVAGHELPAGSEWELSIAYSVAKPRHGAHFVAPDPDEPSQPQMVWTQ
                     SEPEYARFWLPCFDHPADRLTSEIVATVPSRFVTLSNGTLESKQENTDGTTTWHWRQA
                     KSHVPYLLSIVAGDFEVLEQESLGIPVQSYVPRGRLADAPRSFEKTPAMLKLFSEKIG
                     YKYPWPKYAQICVDEYSWGGMEHTSATTLNLNTLHDARAHHDVSSDNLVAHELAHQWF
                     GDLMTCKDWGEIWLNESFATYFATLWTEHDQGWDEAAWDRHEEANSYLGEDGRYRRSI
                     VNFRYTSPINVFDRHAYPKGGRVLHMLRHELGDEQFWRAIRRYVEVNQFRTVETADFR
                     IAIEESTGQGLNWFFDQWLYKGGQPEFNVAWDYDSVAKQVRLTVKQTQKVDDLTPLFR
                     TSAEIEIASGSSTPVMRRVTLSKAEETLHFDAPQRPTRVVFDPRDWILKKLTFDKPKE
                     ELLDQLAHDEHVMARVQAVQGLAGHRDEQDALAGLIATAKDDPFWAVRKEIVKVLGKC
                     KGDEARAALIQAAKSDPKSFVRSEALIALQNFAHDDSRAAARHVVDNDESYFAAAEAL
                     KCLAKIDRENCRDLMLAALEKQSHDEVLLKAACDGLVELKAATASDAIAKLLDGELTP
                     QRRAILISALARLKPDDAEALARVHGLLDNERVQVRRSAIECVASIGNPESIDKLSAR
                     RGKEEVPNVVNSIDEALAKLRERLQQNDSLRKELETLKKQNQQLEERLKKLEQPRTEN
                     "
BASE COUNT          821 a         1466 c         1560 g          981 t
ORIGIN      
        1 cgcggtagtt gcccgaggcg aaaaatgttc cctttggaga ccagaggact gtccgcacac
       61 cgcgcggact ggggatgatg tgcttcgctt cgccggtgac gacgttccag acgcgaactt
      121 cgcccgcttc ggtcgtcttc cagctcccgt gcggcgtgac gagccatttg ccgtccggcg
      181 agaaatcggg gaaccagcaa agggtgacga gtttctcttt tttcgcctct tgcgcatgcg
      241 ctagcggcgg caaaaatgcg agtgtaaaaa tcaaaacgac agcaaaagcc agagaagtgc
      301 gaatcatgcc gactcctctc gaaaagagcc tggtttgatg cagtcaacaa ggtttagaca
      361 ttatgtctac tttaggggcg atcgcaagag tcgcaaaagc tagtgtgccg accgaccaga
      421 tttgacaggc gattgcctcg ttcaacggcg agccgcaggc gaagccgttg cagtcccgac
      481 ggggcgcctt cggctacccg ttcaacgaat tacccgtcaa ttcacgtcgg tcgctctact
      541 acggaaaccg ttcgacctgc aagagattgg caatatctgc cgcggcgttc cagcggcttt
      601 cctgggcgtc gatgtaaccc aggttcactt cgccgagcgt ccgctgcgcc tgcaaaagcc
      661 gcaggaagtc gatctgcccc tgttggtaga gcccttgcgt gagtgaaacc gactcgcgcg
      721 atttcgggag gatttgtgcc tggtagcgtt cgaccgtgtt caacgcggct cggtagcggc
      781 cgagggcctc ggctgcctgt ccggccagtt cgacctgtac gcgtcggaca tccgcctcag
      841 cccggccaac ggtcccccga gcggcccgaa ttccgccctg gttcttgttc cacagcggca
      901 cgctcatcag cacctgagcg atggcttggt cctgaacgcc ctgctcctgg tgctggtagc
      961 cgcccatgat gttgaaggtg ggtcgcggtt cgacataggc ccggtcgagg aggatccgtg
     1021 cgcggcgggc ttccgcttcg gcgacggccg tctgagggtt gacggcgatc accctttgct
     1081 gcagctcggg caaatcgaac cgcggcagtt cggtggccag atcgccgtag agcactccca
     1141 cggccaactg cggcacgcca acagccgcgg ctagctgccg cttgccggcc tcatagacgg
     1201 tgcgggcgtt ttcgagcgcc acctgatcct tgtgcagctc aatttccagc agcagcgtat
     1261 cggttttggt tccttcgccg gcattgaaga gcatttgccc gatcttctgc gacttcgtcg
     1321 ccagtgagac gaggtcttcc aggactgcga cgcgattttg agcagccagc acggcataga
     1381 agcgccgccg cacgcccgtc agcacgtcgt accgcacgcg cgtcattgtc agccgggatt
     1441 gctccacttc ctgccacgcc gctgcggaat cgagttgcag tttgccgccg gtcagaattt
     1501 cctggctggc gaaggcgttg tactgcgatt cgcggccggc gatctgcggc gcggcgacgc
     1561 caacctgcgg attgggatag agccgcgcct ggatcgcctt tccttcggct gcggcgacgg
     1621 cattgactgt ggcgaggagc tgcggatggt tctgcagggc gatctgctcc aggtccgaca
     1681 ggcgaatctc ggctctggcg agttcttccg gcgggcttgg agcttgcgga tcgacctgga
     1741 gtaccggtgg tggcagccgt tccggcggcg gcagagcggg tggcaagcga tggctctgct
     1801 gcggtgcgag cgatggctgc tgagcagcca ggggcgtgct ggcacgcggc gacagcacaa
     1861 tcgccgctgt catcaacaag aaagcggcgg cagcgcaaac gcgacctcct gcgcatgcct
     1921 ccatggtatc ccccttatct gcactatcga caccagcggc aaagcccgtt agggaaaggt
     1981 ccgcgtgcaa cttgccgcaa attgcctagt tttcagtccg cggctgctcg agcttcttca
     2041 gccgctcttc gagttgctgg ttctgctttt tgagtgtttc gagctccttg cggagggagt
     2101 cgttctgctg caggcgctcg cgaagcttgg cgagcgcttc gtcgatcgag ttgacgacat
     2161 tgggaacttc ctccttgccg cggcgtgcgc tgagcttgtc gatcgattcg ggattcccga
     2221 tcgaggccac acattcgatc gcggaccggc ggacctggac gcgctcgttg tccagcaggc
     2281 cgtggacgcg ggccagcgcc tcggcatcgt cgggcttgag gcgggccaaa gctgaaatca
     2341 aaatggcccg ccgctgcgga gtcagctcgc cgtcgagcag cttcgcgatc gcatcgctcg
     2401 cggtggcggc tttcagctcg accagcccgt cgcaggccgc cttcagcagc acctcatcgt
     2461 ggctttgttt ctccagcgcg gccagcatca gatcgcggca gttctcgcgg tcgatcttgg
     2521 ccaggcactt gagcgcctcc gcagccgcga agtacgactc gtcgttgtca acaacgtggc
     2581 gtgctgcggc ccgcgaatca tcgtgggcaa agttctgcaa ggcgatgagc gcctcgctgc
     2641 ggacgaacga cttgggatcg ctcttggccg cttgaatgag cgccgcccgg gcctcatccc
     2701 ccttgcattt gccgaggact ttcacgatct ccttccgcac ggcccaaaac gggtcgtctt
     2761 tggccgtcgc gatcaggccc gccagcgcgt cttgctcgtc cctgtggcca gccaaccctt
     2821 gcaccgcctg cacgcgtgcc atcacgtgct cgtcgtgagc gagctgatcg agcagctctt
     2881 ccttgggctt gtcgaacgtg agctttttca gaatccaatc gcgggggtcg aagaccaccc
     2941 gcgtcggccg ctggggagcg tcgaagtgca gcgtctcctc ggctttgctt agcgtgacgc
     3001 gtcgcatgac cggcgtgctg ctgccgctag cgatctcaat ttcggcagaa gttcggaaga
     3061 ggggcgtcag gtcatcgact ttctgggttt gcttgacggt caagcggacc tgcttcgcca
     3121 cagagtcata atcccaggcg acgttgaatt ccggttgccc acccttgtag agccactggt
     3181 cgaagaacca gttgagcccc tggccggtcg attcctcgat ggcgatccgg aaatcagccg
     3241 tctcgaccgt gcggaactgg ttcacctcca cgtagcggcg gatcgcccgc cagaattgct
     3301 cgtcgccgag ctcgtgccgc agcatgtgca gcacgcggcc ccctttggga taggcgtgcc
     3361 ggtcgaagac attgatcggc gaggtgtagc ggaagttgac gatcgagcgg cgatagcggc
     3421 cgtcctcgcc gagatagctg ttggcttcct cgtgccggtc ccaggccgct tcgtcccagc
     3481 cctggtcgtg ctcggtccag agcgtggcga aataggtggc aaagctctcg ttgagccaga
     3541 tttcgcccca atccttgcac gtcatcaggt cgccgaacca ctggtgggcc aattcgtggg
     3601 cgacgaggtt gtcgctcgat acgtcgtgat gggcccgggc atcgtgcagc gtgttgagat
     3661 tgagcgtggt ggccgacgtg tgctccatgc cgccccagct gtactcatcg acgcagatct
     3721 gggcgtactt gggccaggga tacttgtagc cgatcttctc gctgaagagc ttgagcatgg
     3781 cgggcgtttt ctcgaagctc cgcggggcat cggccagccg gccgcgcggg acgtacgact
     3841 gcacggggat gccgagcgac tcttgctcca gcacctcgaa atcgccggcg acgatcgaca
     3901 gcaaatacgg cacgtgcgat ttcgcctgcc gccagtgcca ggtggtcgtg ccgtcggtgt
     3961 tctcctgctt gctctcgagc gtgccgttgc tgagcgtgac gaaccggctg gggacggtgg
     4021 cgacaatttc gctcgtcagg cggtcggcag gatgatcgaa gcacggcagc caaaagcggg
     4081 catattccgg ctcgctttgg gtccagacca tttgcggctg gctgggctca tccggatcgg
     4141 gagcgacgaa atgcgcgccg tggcggggtt tggcgacgga gtacgcgatc gaaagctccc
     4201 actcgctgcc ggccggcagt tcatggccgg cgacgatcgt cagcgattga tctcgttgcg
     4261 agaacttggg ctcaaccggc gcttgttcgc tggtggccgg aaggggtgaa agcgtgacgc
     4321 tttcgatctt catctcggca gcgtccaggg cgatttcgcg ggccggctgg aacagcgcca
     4381 gttgatgaat ggcgcggccg cggaattcct gcttgtcgaa gtcgaacgtc agctccagcc
     4441 gcacgtgctt ctggtcgatc gtccggctgc ggagcgagcg cggcggctga ttgaatggtc
     4501 cgctgcgctg ctgggccgcg gcggaactgc aagccagaag aagacacacg accagcgaaa
     4561 ggcgcagcga ttgcatggag tgattcctcg gtggatgccg gccaagaggg gggccggcgt
     4621 ttagggttca cgataattgg tcgaatgccg gcttggaatc gggcccttgc cagcgttgca
     4681 gtgactgcgc agatggcgca aatcccgcaa ccgtcatggt ttagggctgt aattcacgcc
     4741 gccagccgat tgtcggcagg cggaagccgg gtaggatatg tttaagcaag gcaactctgg
     4801 attaacgaca caggcgcgat ttcaaccg
//
LOCUS       DASUGB010001115        10399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1654285, whole genome shotgun sequence.
ACCESSION   DASUGB010001115 DASUGB010000000
VERSION     DASUGB010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654285"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFV87_21200"
     CDS             <1..656
                     /locus_tag="VFV87_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106356.1"
                     /translation="RALQYTIEAQHHGGGWRYQPYDAGDMSQFGWQLMALKSAELGGI
                     PIPGATRSRMSNFLRSCSSGKSRGLASYRPGDRVSRTMTAEALVCRYFLATENSPAAI
                     DEAAAFLLQERPGEGPANYYYWYYGTVALFQRQGDDWTRWNAALQGELLNSQRWDGNS
                     VGSWDPSDQWGGYGGRVYSTSLATLSLEVYYRYLPLHIQKRLQERLTERTAFPGLPR"
     gene            976..1698
                     /locus_tag="VFV87_21205"
     CDS             976..1698
                     /locus_tag="VFV87_21205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5106357.1"
                     /translation="MAQQHVLVTGGTSGIGRAIAEAFAREQSLVTVTGRTDRDVTNFL
                     AHPRTEIEISARRMDVTEASDIAAVFRGQDRLDVLINAAGTILREGRELEPDGFEAVV
                     DVNLVGTMRVCTAACELLFTSSGCVINIASMLSFFGSGYVPAYSASKGGIVQLTKSLA
                     IAWAAKGVRVNAIAPGWIETAMTAPLVADPARREAIIARTPLARWGQPEDVAGAALFL
                     ASPAAAYITGIVLPVDGGYSAA"
     gene            1695..2183
                     /locus_tag="VFV87_21210"
     CDS             1695..2183
                     /locus_tag="VFV87_21210"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEX5106358.1"
                     /translation="MSNIDDNCLMIAEMAIPALPEDDRLWVPQAPNVWFRPLLLNTVN
                     GEWVNLLKVTRSGVLSRHRHPAPVHGYVIRGSWRYLEHDWIAREGMYVFEPPGEIHTL
                     VVDPDVPEMITLFHVCGALVYFDDQMRPTGHDDVHTKIDMCRKHFAQVGLGEGYVRQF
                     IR"
     gene            2368..3357
                     /locus_tag="VFV87_21215"
     CDS             2368..3357
                     /locus_tag="VFV87_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX5106359.1"
                     /translation="MNARNKPACPDCDSGLDRRDFLRAAGAAAALGAAVPSLFFPHVA
                     HAAPTASSAAESAVGRLYGSLSEEQKKAICFPFDHELRGKISANWQITKPSIDDKFYS
                     KEQREIIHEIVKNITTPDGYERILKQTEDDNGGMGAYAVAIFGEPGSGKFQWEMTGRH
                     LTMRADGNSVDKAAFGGPIVYGHGEEDPKNNLFHYQTQQTNEVFKALDAKQRKQALVA
                     QAPAESAVDLQGESGKFPGIAVSELSSDQKELVEKTLKVLLAPYRQEDVDEVMEVLKA
                     GGGVDQLHMAFYAQGDLKDDSVWDIWRVEGPSFVWHFRGAPHVHAYINIGIKS"
     gene            complement(3556..5043)
                     /locus_tag="VFV87_21220"
     CDS             complement(3556..5043)
                     /locus_tag="VFV87_21220"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5106360.1"
                     /translation="MRIAAGGILHETSTFLPFPTSLADFEQGFGLYRGAEIPQRFRGA
                     NMCIGGFFDAAEECGFEIVPILWGFAYPSGVISRQDYETIKAEFIVRLAGATAMGSLD
                     GVLLDLHGAMVVEGLEDGDGDMVASVRAAIGPDRPIVVTYDLHGNHTAQRMQRASASI
                     GYDTYPHVDMAERGREAGKLIARAVRGEVRPVTALKQIPLFWSSQRQVTAHEPMNEVL
                     LRIHELESRPGILSASIATGFPWADVPQLGASVFVVADGDERLAQRSADELGEWIWQN
                     RERWQQPPPTIREGLAAGERDGRYPIILGDFNDNTGGGAPGDSTAVLRTFLELNLQDA
                     LLLYLVDVAAAERAHQAGTGAKLKLALGGKSHPAQGEPVELEVEVMALSDGTFRYDGP
                     MYAGLTGNMGQSAWLRYANGDGSGVSIVVVSKREQPLDPAFARSLGIDCAAMKYIAVK
                     SAVHFRSGFEALAGSIHLVDAPAIHSHRFQTLQYHRRPKMYPLER"
     gene            5230..6426
                     /locus_tag="VFV87_21225"
     CDS             5230..6426
                     /locus_tag="VFV87_21225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5106361.1"
                     /translation="MKPASTLLTLLLVAAALHCLVDTVAGTLNPLWPRFETHFQLDGW
                     ETAALFFVWQLTTSVSQFFFGLYGDRFHSRWLLWAGPLVAILCLGSVGLTTSPVVFGL
                     LLVAGGLGIAAFHPEAAALVGSSAPEHRSRAMSVFTMGGFMGQALGPAYSGAMVDSFD
                     LRGLAWGIAGGLMGLVFLAPLVGGAAPARAPTTRPPADLRHLFRGRAGAMLLVLIVGA
                     LRIIAAGGVPVLIGFLLASRGAGTTQTGLVQSAFMLGIGLGGLACATLVRPQHERPIL
                     WLCPLAVTPVLILIPWLSGAVLAATICLAGVLLGISLPVLISYGQQLLPGSQRIASSI
                     TMGVSWGLGGGIVAIILAVCQSSGRFDLAFHLFAGATALSSLLCIWLPAVAPARAVAI
                     AGTVTA"
     gene            complement(6508..7536)
                     /locus_tag="VFV87_21230"
     CDS             complement(6508..7536)
                     /locus_tag="VFV87_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106362.1"
                     /translation="MIRYIFHPLMWLAVIAISTGIAGVAESESPDIAAAPLSGRLFFM
                     TEGRIGSLGANDENPRFHSPEWKPGNPDLLPYWLRVSPDGGQIAFGIVGDQGPGVSLI
                     EATGLYLRGRDATKPAVDTKRLAARWCWSPNGKQLAGSSIERPRGVPEAQHWIMEVPT
                     LTVTSVNLPSDRLITDWSADGNWFLTESAPFVEPRRNSIQLVKRGGTEVRTLSDPKEH
                     AIAARFSPSGRGVLLTTFDFRDRKSNLYVVQIGDDITRRKVSADFFGQVVGACWSPDN
                     KQIASVWRMKYIDPVKEAKAAYSLVVTDADGGNAVAIRTVTGQMPAEETAAHPLSWVT
                     MSALDWQR"
     gene            complement(7571..8971)
                     /locus_tag="VFV87_21235"
     CDS             complement(7571..8971)
                     /locus_tag="VFV87_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106363.1"
                     /translation="MLRQAGTGFGAIALAGLLQQDALRAAALNPDSRTPNPAVRRPHL
                     PPKAKSLIFIFLEGGPSHLDTFDPKPALEKYAGQPLPSSFKPVILAMGEKNPPLLASQ
                     RKWAQHGAGGLWVSDWLPHMATCADDLCVVRSLYSDGLNHSGGVCQMNTGSILAGRPS
                     LGAWVSYGLGTENQNLPAFVVMEDNPGQVINGPKNWGAGFMPAVYQGTAFQNGDEPIQ
                     HLANPKDVTDPRQRAKLQYLAQLNQAHAAERPEQSELEARIASYELAFRMQAEAPGVV
                     DLSDETAETQALYGMDKKETAGFGRQCLLARRLVERGVRFVQLYHGSGSKWDAHSGIE
                     KNHSANCLASDQPVAGLIKDLKRRGLLNDTLVIWGGEFGRTPMSEKGDGRDHNPYGYT
                     MVLAGGGVRGGRTVGATDEIGLHATEDRLHTHDFHATILRLLGLDNMQLTYMHKNRPE
                     RPTLNEGVPCEKIVNG"
     gene            complement(9108..>10399)
                     /locus_tag="VFV87_21240"
     CDS             complement(9108..>10399)
                     /locus_tag="VFV87_21240"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5106364.1"
                     /translation="VAAHEQQLAAWQLQTADLRRQIAEIEDPVRHSASRTTLVKFTDD
                     LQELLAKSPAERTPYEAQLVYLIEFQATDGEGKADIGAKLKGEKKERWQNLKEKLAEF
                     DEMRPQPIAFVPSVTDIGPIAPEVTIPGRRNAEPIAPGILSVLDEGPLAIPPPPSSLT
                     TGRRTALAQWIASPENPLPARVLANRLWQHHFGQGLCQSPSDFGRLGDPPSHPELLDF
                     LAAGLLEHGWTLKRLHRQMVNSAAYRRASHGPASAAAARQDPQNRWLARMSVQRLSAE
                     QIRDAALAASGELDLRMGGEGGDSGKTARRAVYLKVFRNKQDQVLDVFDVPDGISTMP
                     VRNLTTTPTQSLFMINGPWMMLRAKAFARRLSQPSSATLDERIGTAFRLSYGRPPTAD
                     ESRAATDFLQSGAADEKEALVDFCHVLLNSSEFLYVD"
BASE COUNT         1772 a         3302 c         3331 g         1994 t
ORIGIN      
        1 agcgagccct gcaatacacg atcgaggccc agcaccacgg cggcggctgg cgctatcagc
       61 cttatgacgc cggcgacatg agccagttcg gctggcagct catggccctc aagagcgccg
      121 agctgggcgg catcccgatt cccggcgcga cgcgctcccg catgtccaac ttcctccgct
      181 cctgttcctc cggcaaaagc cgcggcctgg cgagctatcg ccctggcgac cgcgtcagcc
      241 gcacgatgac cgccgaggcc ctcgtctgcc gctatttcct cgccaccgag aacagccctg
      301 ccgcgatcga cgaagccgcg gcgttcctgc tccaggagcg gcccggcgaa ggcccggcga
      361 actattacta ctggtactac ggcacggtcg ccctcttcca gcggcaaggc gatgactgga
      421 cgcgctggaa cgccgccctg cagggcgagc tactcaatag ccaacgctgg gacggcaact
      481 cggtcggaag ctgggacccg agcgaccagt ggggcggcta cggtggccgc gtctatagca
      541 cttccctggc cacgctctcc ctcgaggtct attaccgcta cctcccgctg cacatccaaa
      601 agcgcctgca ggagcggctc accgaacgca ccgccttccc cggcttgccg cggtaaatac
      661 gatcgtcggg gtggaattta ccatcctggc tcattctcaa ccctcccgtt gcgctctttt
      721 ttgacgtgga gcaggggaat cgagctgaga tctcggcagg gatagccaaa ctgtaaacgt
      781 ccaagcacat ttccagtccc gcggacgttc acccaatcat ccgttgctac catccgttaa
      841 acgcatcctt gtgggcacat ctggtccatt tggtcaagca agaaagactg tattttctgg
      901 gtgcgacaat gatcgccgca cggccgtgcc actcgaggga gcacgccatc aacgcgcggg
      961 actatcatgg gacgcatggc ccagcaacac gttctcgtca ccggtggaac cagcggcatt
     1021 gggcgggcga ttgcggaggc gtttgcgcgc gagcaatcgc tcgtgacggt gaccgggcgg
     1081 accgacaggg atgtgaccaa tttcctggct catccacgga ccgaaattga aatctccgcc
     1141 cggcggatgg atgtgaccga ggcttcggat attgcggcag ttttccgcgg gcaggaccga
     1201 ctggatgtct tgatcaacgc tgcaggcacg atcctgcgcg aaggccgtga actggagccc
     1261 gatggctttg aggcggtcgt cgatgtgaac ctcgtcggca cgatgcgagt ctgcaccgcc
     1321 gcctgcgaat tgcttttcac cagcagcggg tgcgtcatca atatcgcctc catgctcagc
     1381 tttttcggca gcggctacgt gccggcctat agcgcgagca aaggaggcat tgtgcagctc
     1441 accaaatcgc tcgccatcgc ttgggctgcg aagggcgtgc gggtcaacgc gattgctccc
     1501 ggctggattg aaacggcgat gaccgcgccc ctcgtcgccg atccagctcg gcgggaagcg
     1561 attatcgccc gcacgccgct cgcccgctgg ggccagccgg aggacgtcgc cggcgcggcc
     1621 ctgttccttg catcccccgc tgcggcttat atcaccggta tcgtcttgcc ggtcgatgga
     1681 ggatactcag cggcatgagc aacatcgacg acaattgcct gatgattgcc gagatggcca
     1741 tcccggccct gcccgaggac gaccgcctgt gggtcccgca agcgccgaat gtttggtttc
     1801 gccccctgct gctcaacacc gtcaacggcg agtgggtgaa tctcctcaaa gtgacgcgga
     1861 gcggcgtcct cagccggcat cgacatccgg cgccggtcca cggctacgtg atccgcggct
     1921 cgtggcggta cctggagcac gactggatcg cccgcgaggg gatgtacgtc ttcgagccgc
     1981 cgggcgaaat ccacacgctc gtggtcgatc cggacgtgcc cgagatgatc acgctattcc
     2041 acgtctgcgg ggcgctcgtc tatttcgacg accagatgcg ccccactggc cacgacgacg
     2101 tccacaccaa aatcgacatg tgccgcaagc actttgccca ggtcggcctc ggcgaaggct
     2161 acgtgcggca gttcatccgc tgaccgtaac ccgaagcgtc ggcgcgggaa tccggttgtg
     2221 aggcgtcagc gacggggttc gtcgccaatg tggcactccc tcgcttacgc ttcaagttgg
     2281 tgtatttgct gattgccaac cgctttgccg gggcgctata atcgcggcag tcggcccgcc
     2341 catccctatc ctctccagga gcccgccatg aacgcgcgaa acaagcccgc ttgcccggat
     2401 tgcgattctg gactcgatcg tcgcgatttc ctccgcgcgg ccggcgctgc ggctgccctc
     2461 ggcgctgctg ttcccagctt gttctttccc cacgtagctc acgcggcgcc caccgcttct
     2521 agcgcggcgg aatcggcggt cggccggctc tacggctcgc tttcggagga gcagaagaag
     2581 gccatctgct tccccttcga ccacgagctg cggggcaaga tcagcgccaa ctggcaaatc
     2641 accaagcctt cgatcgacga caaattttac agcaaggagc agcgcgagat tatccatgaa
     2701 atcgtgaaga acatcacgac gccggacggc tacgagcgga tcctcaagca gaccgaagac
     2761 gacaacggcg gcatgggggc ctatgccgtc gccattttcg gcgagcctgg cagcggcaag
     2821 ttccagtggg aaatgaccgg ccgccacctg acgatgcggg ccgacggcaa cagcgtcgat
     2881 aaagctgcgt tcgggggacc gattgtctat ggccacggcg aggaagaccc caagaacaac
     2941 ctgttccact atcagacgca gcagaccaac gaggtcttca aggccttgga cgccaaacag
     3001 cgcaagcagg cgctcgtcgc tcaggcgccc gctgaaagtg ccgtcgatct ccaaggcgag
     3061 agcggcaagt tcccgggcat cgccgtcagc gagctgagca gcgatcagaa agagctggtc
     3121 gaaaagacgc tcaaggtgct cttggcgccg tatcgccagg aagacgtgga cgaagtgatg
     3181 gaagtgctga aagccggcgg tggcgtcgat cagctgcaca tggcgttcta tgcccaggga
     3241 gacctgaagg acgacagcgt ctgggacatc tggcgggtcg aaggcccgtc gttcgtctgg
     3301 cacttccggg gcgcgccaca cgtgcatgcg tatatcaaca tcggaatcaa aagctgatct
     3361 ctgccgatcg accgttccga gcaatcgccg gggcctcccg tagattcggg aggcctacat
     3421 tggtgatgcg gttggtttgc aagcggcgcg ttgcggccgc cgcggcgtcg taagcgaagc
     3481 gacgttctgc tgtcgcgcgt gcgctgcagc gcgcgtgcca cctacttcag ggagtctcat
     3541 ggtgtacgac gcctactacc gttccagagg atacatcttc ggccggcggt ggtattggag
     3601 cgtctggaac cggtggctgt ggatcgccgg ggcatcgacg aggtgaatcg agccggctag
     3661 cgcctcgaag ccgctgcgga agtggacggc gcttttcacg gcgatgtact tcatagccgc
     3721 acagtcgatg cccagcgagc gcgcgaaagc cggatccagc ggctgctcgc gcttggagac
     3781 gacgacgatc gagacgccgc tgccgtcgcc gttggcataa cgcagccagg cgctctggcc
     3841 catgttgccg gtgagtccgg catacatcgg gccgtcgtac cggaatgtgc cgtccgagag
     3901 ggccatgacc tcaacctcca gctcgaccgg ctcgccctgg gccggatgcg acttgccgcc
     3961 cagcgccagc ttgagctttg cgcctgtgcc ggcctggtgt gcccgctcgg ctgctgcgac
     4021 atcgaccaga tacagcagca aggcgtcctg cagattcaac tccaagaacg tccgcaacac
     4081 cgctgtcgaa tcgcccggcg ctccgccgcc ggtgttgtcg ttgaagtcgc ccaggatgat
     4141 cgggtagcgg ccgtctcttt cgccggcggc caggccttcg cgaatggtcg gcggcggctg
     4201 ctgccagcgc tcgcggttct gccagatcca ctcgcccagc tcgtcggctg atcgctgagc
     4261 tagccgctcg tcgccgtcgg caacgacgaa gacgctagcg ccgagttgcg gcacgtcggc
     4321 ccacggaaac ccggtggcga tgctggccga caaaatgccc ggccgcgatt ccaactcgtg
     4381 aatccgcagc agcacctcgt tcatcggctc gtgagccgtc acttgccgct gtgagctcca
     4441 gaagagtgga atctgcttga gcgccgtgac ggggcgtacc tcgccgcgga ccgcgcgggc
     4501 gatcagcttg cccgcttcgc ggccgcgctc ggccatatcg acgtgcggat aggtgtcgta
     4561 gccgatgctg gcactggccc gttgcatgcg ctgggccgtg tggttgccgt gcaggtcgta
     4621 agtgacgacg atcggccggt ccggaccgat ggccgctcgg accgacgcga ccatgtcgcc
     4681 gtcgccgtct tccagccctt ccaccaccat tgctccgtgc agatcgagca agacgccatc
     4741 caagctgccc atcgccgtcg ccccggccaa acgcacgatg aactccgcct ttatcgtctc
     4801 atagtcctgc cgcgagatca caccgctggg ataggcaaat ccccatagga tcggaacaat
     4861 ttcaaagccg cactcctcgg cggcgtcgaa gaacccgccg atgcacatat tcgctccgcg
     4921 gaaccgctgc ggtatttccg cgccgcgata gaggccaaaa ccctgctcga aatcggccag
     4981 gcttgtcgga aacggcaaaa acgtgctcgt ttcgtgcaag atcccgccgg ctgcaattcg
     5041 catggtgaac cttcctcaaa agcgagtgtc caacgcagtc cgagtccagc ctactgtagc
     5101 cgaagtcggg agacggaggg ccgagcacgc cttccgggac cgatctgtca tgagacggct
     5161 acgtccctgg ccaggacaac tctgtcggga tagagttgtc ctttgcccgc cgcgccgttt
     5221 gcctcaccga tgaaacccgc ctccacgctt ctgacgctgc tcctggttgc cgcggcgctg
     5281 cactgcctgg tcgatacggt cgccgggacg ctcaatccgc tctggccgcg attcgagaca
     5341 cacttccaac tcgacggctg ggagacggcg gcgttgttct ttgtctggca attgacgacg
     5401 tcggtcagcc agtttttctt cggcctttac ggcgaccggt ttcacagtcg ctggctcctc
     5461 tgggcgggac cgctggtggc gattctgtgc ctgggaagcg tgggattgac gacgtcgccg
     5521 gtcgtgtttg gcctgctcct tgtcgcggga gggctgggta ttgccgcttt tcacccggaa
     5581 gcggccgccc tcgtgggcag cagcgctccg gagcaccgca gccgggccat gtcggtcttc
     5641 acgatgggag gcttcatggg gcaggcgctc ggcccggcct acagcggtgc gatggtcgat
     5701 tcgttcgact tgcgcgggct cgcctggggg attgccggcg gtttgatggg cctggttttc
     5761 ctcgcgccgc tcgtcggcgg ggcagcgccg gcgcgagcgc cgacaactcg accgccggcc
     5821 gatctccgtc atttatttcg gggccgcgcg ggagcgatgc tgctggtcct tatcgttggt
     5881 gcgctgcgaa tcattgcggc cggcggcgtg cccgtgttga tcggctttct gctcgcttcg
     5941 cgcggagcgg gtaccacaca gacgggcctg gtgcaatcgg ccttcatgct gggaattggg
     6001 ttggggggcc tcgcgtgtgc gacgctggtc cgtccgcaac acgagcggcc gatcctgtgg
     6061 ctttgcccgc tggcggtgac gcccgtgctg atcctgattc cgtggctgag cggtgcagtt
     6121 ctagcggcca cgatttgcct ggcgggagtg cttctgggga tctcgctgcc ggtgctgatc
     6181 agctacgggc agcagctcct gccggggagc cagcggatcg ccagctcgat cacgatgggc
     6241 gtaagctggg gcctgggcgg aggcatcgtg gcgatcatcc tcgccgtctg ccagtcgtcc
     6301 ggccggttcg atctagcttt tcacctgttc gccggagcga cggccctttc cagtctgctc
     6361 tgcatctggc tgccggcggt cgcaccggct cgcgccgttg ccattgccgg aacggtcacg
     6421 gcttaacgcc cgcctgtggg ctcaacgtta ggctcccacg atcttctcgc acggcacgcc
     6481 ttcgttgagc gtggggcgct cggggggtta cctttgccag tccaaagcgg acatcgtgac
     6541 ccaactcaac ggatgagcgg ccgtttcttc ggcaggcatt tgccctgtca ccgttcggat
     6601 ggcgactgca ttgccgccat ccgcgtccgt taccaccaaa gaatatgcgg cctttgcctc
     6661 ttttactgga tcgatgtatt tcatgcgcca aactgacgcg atctgcttgt tatccggtga
     6721 ccaacacgcg ccgacaactt ggccgaaaaa gtcggcgctg acttttcgac gggttatgtc
     6781 atccccgatt tgcaccacgt acaaattgct ctttcggtca cggaagtcaa aggtcgtgag
     6841 caggacgcct cgcccactgg gtgaaaaccg cgcggcgatg gcatgttctt tcggatcact
     6901 caacgtgcga acctccgtgc cgccgcgttt gacgagttga atggaattgc ggcgaggctc
     6961 gacgaaaggc gcggactctg tgaggaacca attgccgtct gccgaccagt cggtgatcag
     7021 tcggtcggac ggcagattta cacttgttac cgtaagagtc ggcacttcca tgatccaatg
     7081 ctgtgcctca ggcactcccc gaggtcgttc gatgctgctt ccggcgagtt gctttccatt
     7141 gggtgaccag caccatcttg ccgcgaggcg tttcgtatca acagcgggct tagttgcatc
     7201 tcgtccccgt agatacaaac cagtcgcttc gattaacgac acacccggac cttgatctcc
     7261 gacaattcca aacgcgatct gtccaccgtc aggtgaaact cgtagccaat acggcaagag
     7321 atcgggattt cctggcttcc actccggcga gtgaaatctt gggttctcgt catttgctcc
     7381 gagcgagcca attcgccctt ctgtcatgaa gaacagtcgc cctgacaacg gggccgccgc
     7441 aatatccggt gattcagatt ctgcaacacc agcaatccca gttgaaattg caattaccgc
     7501 cagccacatc aaggggtgaa aaatgtaccg aatcatggac ggcctcattc caagaaaagg
     7561 cgtgagccag ttatccattc acgatcttct cgcacggcac gccttcgttg agcgtggggc
     7621 gctcggggcg gttcttgtgc atatacgtga gctgcatgtt atcgaggccc agcagccgca
     7681 ggatcgtggc gtggaagtcg tgcgtgtgga ggcggtcctc ggtggcgtgg aggccaattt
     7741 cgtcggtcgc cccgacggtg cggccgccgc ggacgccgcc gccggccagc accatcgtgt
     7801 agccgtaggg gttgtggtcg cgaccgtcgc ctttttcact catcggcgtg cgaccgaatt
     7861 cgccgcccca gatgacgagc gtgtcgttga gcaggccgcg gcgcttgagg tccttgatca
     7921 gtcccgcgac gggctggtcg ctggcgaggc agttggcgga atgattcttc tcgatgccgg
     7981 aatgggcgtc ccacttgctt cccgagccgt gatagagctg cacgaatcgc acgccgcgct
     8041 cgaccaggcg gcgggccaag aggcactgcc ggccgaagcc ggctgtctcc ttcttgtcca
     8101 tgccgtagag ggcctgcgtt tcggccgttt cgtccgacaa atcgacgacg ccgggggcct
     8161 cggcttgcat gcggaaggcc aattcatagc tggcgatgcg agcttcgagc tccgattgct
     8221 cgggtcgctc ggcagcgtgt gcctggttga gctgcgcgag gtattggagc ttggcccgct
     8281 gccgcggatc ggtcacgtcc ttgggattgg ccaggtgctg gatcggctcg tcaccgtttt
     8341 gaaaggcggt gccttgatat accgcgggca tgaacccggc tccccagttc ttcggaccgt
     8401 tgatcacctg gccggggttg tcctccataa cgacgaatgc cggcagattc tggttctcgg
     8461 tccccaaacc atagctgacc cacgcgccga gagacggccg gccggcgagg atcgagccgg
     8521 tgttcatctg gcagacgccg ccggaatggt tgaggccgtc cgaatagagc gagcggacga
     8581 cgcacaggtc gtcggcacaa gtggccatgt gcggcagcca gtcgctgacc cacaggccgc
     8641 cggctccgtg ctgggcccac ttccgctggc tggccaagag cggcgggttt ttttcaccca
     8701 tcgccagaat taccggcttg aacgacgagg ggagcggctg acccgcgtat ttctccaggg
     8761 ccggcttggg atcgaacgtg tcgaggtggc tcggcccccc ttcgaggaaa atgaagatca
     8821 gcgacttggc cttgggcggc aggtgcgggc ggcgaacagc agggttcggc gttcgggaat
     8881 ccgggttcag cgcggcggcg cgcagggcgt cttgctgcaa caggccggcc agggcgatgg
     8941 cgccgaagcc ggtgccggcc tggcgcagca tgtcgcggcg cgtcaggtag gagctcggag
     9001 ctcggagctc ggagtgcgga gcggcaaaca tgctgtcagg aagcggagac agttgtagaa
     9061 ttgggatttg atttggttct tggttcttgg atctttgttc tttgccgtta gtccacgtac
     9121 aaaaactcgc tcgagttcaa aaggacgtgg caaaaatcga ccagggcctc tttttcgtcg
     9181 gctgcccccg attgcaggaa atcggtggcg gcgcgggatt cgtccgcggt gggcgggcgg
     9241 ccgtaggaca ggcgaaacgc tgtgccgatt cgctcatcga gcgtggccga cgacggctgg
     9301 ctgaggcggc gggcaaaggc cttggcccgc agcatcatcc acgggccgtt gatcatgaac
     9361 agcgactgcg tcggcgtcgt ggtgaggttc cgcacgggca tcgtcgagat gccgtccggc
     9421 acgtcgaaca cgtcaaggac ctggtcctgc ttattgcgga agactttgag gtaaaccgcc
     9481 cggcgggccg tttttccgga atccccgccc tcgccgccca tccgcaggtc gagctcgccg
     9541 ctggcagcca gcgccgcgtc gcgaatctgc tcggccgaca gccgctgcac gctcatgcgg
     9601 gccagccagc ggttctgcgg gtcttgccgg gcggcggcgg ccgacgccgg gccgtgcgag
     9661 gcgcgccggt acgcggccga gttgaccatt tgccggtgca ggcgcttgag cgtccagccg
     9721 tgctccagca gccctgccgc caggaaatcg agcagctcgg ggtggctcgg cgggtcgccg
     9781 aggcggccaa aatcgctcgg cgactggcag aggccttggc caaaatggtg ctgccacagg
     9841 cgattggcca gcacccgcgc ggggagcggg ttttcgggcg aggcgatcca ctgggccagg
     9901 gccgtgcgcc ggccggtggt caaggaggaa ggtggcggcg gaattgccag cggcccttcg
     9961 tcgagcacgc tcaagatgcc gggggcaatc ggttcggcat tgcggcggcc gggaatcgtg
    10021 acttccggcg caatcggccc gatgtcggtg acgctgggga caaacgcaat cggctgtggc
    10081 cgcatttcat cgaactcggc cagcttctcc ttaagattct gccaccgctc ttttttctcc
    10141 cccttcagct tcgcgccgat gtcggccttt ccctcgccgt cggtggcttg aaattcgatc
    10201 agatagacaa gctgcgcctc gtagggcgtg cgctcggccg gcgacttggc cagcagctcc
    10261 tgcaaatcgt cggtaaactt gacgagcgtc gtgcgcgagg cgctgtgccg cacggggtcc
    10321 tcaatctcgg cgatctgccg gcggaggtcg gccgtctgca actgccacgc ggcgagctgc
    10381 tgctcgtgcg ccgccactt
//
LOCUS       DASUGB010001116         9014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_402112, whole genome shotgun sequence.
ACCESSION   DASUGB010001116 DASUGB010000000
VERSION     DASUGB010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402112"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1367
                     /gene="bioF"
                     /locus_tag="VFV87_21245"
     CDS             168..1367
                     /gene="bioF"
                     /locus_tag="VFV87_21245"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX5106365.1"
                     /translation="MRRYHGGMPDPLDWLDDALNDLDRRGLRRSLTARTGPQRGDKIE
                     IGGQTLVNFGSNDYLGLAADPRVAAAAKRAIDEYGWGSGASPLVTGRSELHAELERQL
                     AEFEGTEAALLFSSGYAANVGAICALVGKGDAIYSDELNHASIIDGCRLSGATIHVYH
                     HCDVDHLRDLLASGGREPPEARHRLIVTDSLFSMHGDAAPLVELVKIAREHRAMLLVD
                     EAHATGVLGQHGRGVCEELRIEEGISIRVGTLSKALGSHGGFVAGSQRLIDWLANRAR
                     PYVFSTAAPPAVVAAALAALEIVSNEPERRQKLDQLAVTLRRHLVLKDLIQPTSLSHI
                     IPIILGHANRTMAAAAELRRRGFFVPGIRPPSVPEGQSLLRISLTCLHTQEQLDALVD
                     ALCEVAR"
     gene            complement(1410..3503)
                     /locus_tag="VFV87_21250"
     CDS             complement(1410..3503)
                     /locus_tag="VFV87_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5106366.1"
                     /translation="MTPVSDRLAAMLAYLVIRDGTKWTDVYRLVPGRTVTVGRAATNQ
                     IVIKDERASRNHAEIFLTRGNWTLRDLDSRNGTFVGERQVRGDYTLAPGDVVRIAQCQ
                     LAFVQDLSQAFPDPVTGAAGLAQLPIGEETVLGAIVADTGEIDASDVLAEPLEPQTIT
                     HRRGQTRFLVPGGPADVPVPRLGQAAATLCRLAFELASQPDVSSVAHFALNGLFESTH
                     VDAGAVLLLPRGHNGDVVPADLQVVASRTDLEPTYHRPSTFLSSTTLREGEAVLARNI
                     EGDSQLATKDSKGQFHANAAILAPIRQDRKVIGLIHLYSTSDERVPDPDDLEFTLAVA
                     DNVALAIRTLAKQQELAENLSQTRSEILELRKQLGAESELVGSGPVLAGVQQQIMRAA
                     PSRATVLIRGESGVGKELVARAMHYASPRKKGPFVCLNCAALTETLLESELFGHERGA
                     FTGATERKIGKFEAAHQGTIMLDEIGEMSPSIQSKFLRVLEGHPFERVGGSQPIKVDV
                     RVIAATNRDLEKAVAEGNFRRDLYFRLRVVEIFVPPLRKRPEDIIELAEYFLDRYNAE
                     TGRKIRGFTPEAIHQMQQYRWPGNVRELKNVIERAVVLSRTDFIDSEDLNLSSIATVS
                     ESGEAAPSPKTTFEPLSLDDLEKRHIHATLRATGWNKSRTAAILGVERSTLDRKIRRY
                     NLKPPTIPGEQWQAD"
     gene            3755..5110
                     /locus_tag="VFV87_21255"
     CDS             3755..5110
                     /locus_tag="VFV87_21255"
                     /inference="COORDINATES: protein motif:HMM:NF019269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HEX5106367.1"
                     /translation="MASRVVRLGASRCVALAAVVSLAAASLSFAGHGGRIAAVGGVSI
                     DPSGVVEIADVKLKKDARDAALKEYKPAPAELNAPAELRKISLRAIEEAVAKAGKDHA
                     YELPDEIRFLAGIQRIQYVLVYPEQNDIVIAGPGEGWKLDDAGNYIGVTTGRPVLRLE
                     DLIVALRTVENARQGGISVSIDPTAEGRQAFEKFMATQRTFNPGVLGGIEKALGQQDI
                     KITGVPNTSNFARVLVAADFRMKRLAMNLDPTPLKELPSFLNLLKQGGKLDNMMPRWW
                     LACNYEPLAKSEDGLAWELRGPGVKVMTEDEIIGADGSVKGTGKTNPVAQKWSDLMTQ
                     NYDALSAKEPVFGELRNLMDLCVISALISKEGMLEKAGLELPTILGENSKLELASNYA
                     PKSIGTQCSFVKRDREYLITASGGVDIASWEVASKAVTEPSVGEIRQKAAPKTGGMWW
                     N"
     gene            complement(5311..5868)
                     /locus_tag="VFV87_21260"
     CDS             complement(5311..5868)
                     /locus_tag="VFV87_21260"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5106368.1"
                     /translation="MRPLRYAINVTLDGCVDHRAGSTDEELHRYWTENLARADALIFG
                     RVTYEMMEAAWRPAASGVRPEWMTEWMEPFARMIDVAKKYVVSSTLERVDWNAELVRG
                     DLGKAVEQLKREPGQGLFVGGVKLPQALAELGLIDEYEFVVHPRLAGHGPTLFAGLSK
                     YVDLKLVGRREFGSGAVALRYEPRR"
     gene            complement(6250..6675)
                     /locus_tag="VFV87_21265"
     CDS             complement(6250..6675)
                     /locus_tag="VFV87_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106369.1"
                     /translation="MRSMRYRLHTLLIVLAVGPPVLAAIGIAVFSLRPTSPAVFGQVT
                     FRGRPAQLVQMTFLRAAGDGAYSTVTDLEGNYRLKDDSSGRPIRPGAHFVLFYANRSA
                     RSALANYPASLPGEYGNAKNPQVTAAFLPGACRVDFDLK"
     gene            7328..>9014
                     /locus_tag="VFV87_21270"
     CDS             7328..>9014
                     /locus_tag="VFV87_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX5106370.1"
                     /translation="MRFWSVVVPFSFALAALSQMAGSAPGQEQAAGAKKRVLFIAGRP
                     SHGYGAHEHNAGCTLLAKELQAAQPDIKCDVVLNGWPADSSVFDGADCIVMYCDGGGG
                     HMVNPNLEQVDKLAKKGVGIVCIHYGVEVPKGPSGDKFLDWIGGYFETNWSVNPHWTA
                     KYEKFPDHPISRGVQPFEINDEWYYHMRFREGMKGVTPILTALPPKDTLSRPDGTHSG
                     NPAVREAIAKGEPQHMAWAAEREGGGRGFGFTGGHDHWNWGDPNFRKVMLNAIVWCAH
                     GDVPTSGVESPTVTLSTLEVNQDEPAPANFDREAIRKRNKLPADGDADSKTNAEGAAA
                     RQPGGRRGQRATAGGSRAKPIFSSPVVSAATKGHAVEIDADITGAKRLWLVVTDGGDG
                     FGCDWADWAEPRLIGPKGEKKLTEISWKSASAGFGNVNVNKRCDGKDMKINGQSVEYG
                     IGTHANSVIQFDIPEGYTRFKARGGIDNGGTDQGCGSTVQFLVYTQAPPANVASVAGA
                     NNPVRDAENAVANLDVADGLEATLFASEPDISNITNIDIDHLGRVWACEVKNYR"
BASE COUNT         1635 a         2925 c         2915 g         1539 t
ORIGIN      
        1 ccgccgcccc cgcgccgccc gccaatccag cccccgtcga agagccacga accgtcaccg
       61 tcggcccgcc gccgcggggg tagaaacgaa aaaggggtcg ggagtcaatt gcggtccccg
      121 tcatccacta ggaagatcgt cgctgcaatt gactcccgac cccttcgatg cggcgttacc
      181 atggcgggat gcccgatccg ctcgattggc tcgatgacgc cctgaacgac ttggaccgcc
      241 gcggcttgcg gcggtcgctg accgcgcgca ctggcccaca gcgcggggat aagatcgaga
      301 tcggcgggca aacgctcgtc aacttcggct cgaatgacta cctgggcctc gccgccgatc
      361 cgcgggttgc tgccgcggcg aaacgagcga tcgacgagta cggctggggc tccggcgcca
      421 gccccttggt caccggccgc agcgagctgc acgccgagct ggaacgccag ctcgccgagt
      481 tcgaaggcac cgaagcggcc ctgctgttct ccagcggcta cgcggccaac gtcggcgcga
      541 tctgcgccct cgtcggcaag ggggacgcga tctacagcga cgagctgaac cacgccagca
      601 tcatcgacgg ctgccgcctg agcggggcga caattcatgt ttaccaccac tgcgacgtcg
      661 atcacttgcg cgaccttctg gcgagcgggg ggcgtgagcc ccctgaggca cgccaccgtc
      721 tgatcgtcac cgactcgctc ttctcgatgc acggcgacgc cgctccgctt gtcgaactgg
      781 tcaaaattgc ccgtgagcat cgcgccatgc tcctcgtgga cgaggcgcat gccacggggg
      841 tgcttggtca acacggtcgt ggagtatgcg aggaactgag aatcgaagaa ggcatctcga
      901 tccgcgtcgg cacgctcagc aaagcactcg ggagccacgg cggattcgtc gcgggttcgc
      961 agcgtctaat tgactggtta gcgaatcgcg cccgccccta cgtgttctcc accgctgcgc
     1021 cacccgccgt tgtcgcggct gcgcttgccg cactggagat cgtgagcaac gagccagagc
     1081 gccgtcagaa gctcgaccaa ttggccgtta ctctgcgacg tcatctggtt ttgaaggatc
     1141 tcatccagcc aacatcgctc agccacatca tccccatcat cctcggccac gcgaaccgca
     1201 cgatggctgc ggctgcggag ctgcgccggc gggggttctt cgtccccggc atccgcccgc
     1261 cgagcgtacc ggagggacaa tcgctgctgc gaatcagcct cacctgcctg cacacccagg
     1321 agcaactcga cgccctcgtg gacgccctct gcgaagtcgc gcgataggct ctcgccagcc
     1381 gtatccggga gcaggccgcc agcgcctctt caatccgcct gccactgctc gccggggatg
     1441 gtgggcggct tgaggttgta gcggcggatt ttgcggtcga gcgtcgaacg ctccacgccg
     1501 agaatcgcgg cggtgcggct tttgttccag ccggtggcgc ggagggtggc gtggatgtgc
     1561 cgcttttcca ggtcgtcgag cgagaggggc tcgaacgtgg tcttcggcga gggggccgcc
     1621 tcgcccgatt cgctgaccgt cgcaatgctc gacaggttga gatcttccga gtcgataaaa
     1681 tcggtccgcg agaggacgac cgcgcgctcg atgacgttct tcagctcgcg gacgttgccc
     1741 ggccagcgat actgctgcat ctggtggatc gcttccggcg tgaagccgcg gattttgcgg
     1801 cccgtttcgg cgttgtagcg gtcgaggaaa tactcggcca gctcgatgat gtcctccggc
     1861 cgcttacgca gcggcgggac gaagatttcc acgacgcgga ggcggaaata gaggtcgcgg
     1921 cggaagttgc cttcggccac ggctttttcc aggtcgcggt tggtcgccgc aatgacccgc
     1981 acatcgacct tgatcggctg gctgccgccg acgcgctcga aggggtgccc ttcgagcaca
     2041 cggaggaact tcgactgaat tgacgggctc atctcgccga tttcgtcgag catgattgtc
     2101 ccctgatgag ccgcctcaaa cttaccgatc ttccgctcgg tcgcgccggt gaacgctccg
     2161 cgctcgtggc cgaagagctc gctctccagc aaggtctcgg tgagggcggc gcagttgagg
     2221 cagacgaacg gccctttctt ccgcggactg gcgtagtgca tggcccgcgc gaccagttcc
     2281 ttgcccacgc cgctttcgcc gcggatcagc acggtggccc ggctgggggc ggcccgcatg
     2341 atctgctgct gcacgccggc cagcaccggc ccgctgccga ccagctcgct ttcggcgccg
     2401 agctgtttgc ggagctcgag gatttcgctg cgggtctgcg agaggttctc ggccagctcc
     2461 tgctgcttgg cgagcgtgcg gatagcgagc gcgacgttgt cggccacggc cagcgtgaac
     2521 tccaggtcgt ccgggtcggg aacccgctcg tcgctcgtcg aatacagatg aatcagcccg
     2581 atcaccttgc ggtcctggcg gatcggggcg aggatcgcgg cgttggcgtg gaactggccc
     2641 ttgctgtcct tcgtcgcgag ctggctgtcg ccttcgatgt tgcgggccag aaccgcctca
     2701 ccctcgcgga gcgtcgtgga agacagaaaa gtcgagggtc ggtgataggt cggctccaga
     2761 tcggtccgcg acgcaaccac ctgcagatcg gccggcacga cgtcgccatt gtgtccccgc
     2821 ggcaacagca gcaccgcgcc cgcatcgacg tgcgtgctct caaagaggcc gttcagggcg
     2881 aagtgtgcga ccgacgaaac gtccggctgg ctggccagct cgaacgccag gcggcaaagc
     2941 gtggcggcgg cctgccccag ccgcggcacg ggcacgtccg ccggcccgcc ggggacgagg
     3001 aaccgcgttt gcccgcggcg gtgcgtgatc gtctgcggct ccagcggctc ggcgaggaca
     3061 tccgaggcgt cgatctcgcc cgtgtcggcg acgatcgcgc ccaggaccgt ctcttcgccg
     3121 atcggaagct gcgccaggcc cgccgcgccg gtgaccggat cgggaaaggc ctgcgaaaga
     3181 tcttgcacga aggcaagctg gcactgggcg atgcggacga cgtcacccgg cgcgagcgtg
     3241 tagtcgccgc gcacctgacg ctcgccgacg aacgtgccgt tgcggctatc gaggtcgcgg
     3301 agagtccagt tgccgcgggt gaggaagatt tcggcgtgat tgcggctggc gcgttcgtcc
     3361 ttgatgacga tctggttggt ggcggcgcgt ccgacggtta cggtccggcc ggggacgagg
     3421 cgataaacgt ctgtccattt cgtgccgtcg cgaatgacga ggtacgccag catcgccgcc
     3481 aatctgtccg aaaccggagt caagacgagg gtcggcctag cctttcggcc agcctcagaa
     3541 ccccacctta gcctgccgcc cgccgggcgg gcaagtagcg agttttgttg caaagctgcg
     3601 agctggcagt tatcctgatt cctaagtccg ccaaggtttt ccaggggcgt cggacacggg
     3661 aaattgcgcg ccgcatcggc tggccgttcg cagcacgaac ggggccgcca gacccaagcg
     3721 cgctagagcc tgaattcact tcggagtttc tgtcatggca agtcgagttg tgcggctggg
     3781 tgcttcgcgc tgtgtggcgc tggcggcggt tgtttcgctg gcagccgcaa gcctcagctt
     3841 tgcggggcac ggcgggcgaa tcgcggcggt cggcggcgta tcgatcgatc cgtcgggggt
     3901 tgtcgaaatc gccgacgtga agctgaaaaa agacgcccgc gacgccgcgc tgaaggaata
     3961 caagccggcc ccggctgagc tgaacgctcc ggcggagctg cggaagattt cgctgcgggc
     4021 gatcgaggaa gcggtggcca aggcgggcaa ggaccacgcc tacgagctgc ccgacgagat
     4081 tcgcttcctg gcgggcatcc agcggattca gtacgtcctg gtctatccgg agcagaacga
     4141 catcgtcatc gccggcccgg gcgaaggctg gaagctcgac gacgccggca actacatcgg
     4201 cgtgaccacc ggacggccgg tgctgcggct ggaggatttg attgtcgcgc tgcggacggt
     4261 tgagaacgcc cgccagggcg gcatcagcgt ctcgatcgac ccgaccgccg aaggccggca
     4321 agcctttgag aaattcatgg ccacgcagcg gacgttcaat ccgggcgtgc tgggcggaat
     4381 cgagaaggcc ctgggccaac aggatatcaa gatcaccggc gtgccgaaca cgagcaactt
     4441 cgcccgcgtc ctcgtcgccg ccgatttccg catgaagcgg ctggcgatga acctcgatcc
     4501 gacgccgctg aaagagctgc ccagcttcct caatctgctc aagcaagggg gcaagctcga
     4561 caacatgatg ccccgctggt ggctggcctg caattatgag ccgttggcca agagcgaaga
     4621 cggcctggcg tgggagctgc gcggccccgg cgtgaaggtg atgaccgagg acgagatcat
     4681 cggcgctgat ggcagcgtga aaggaacggg caagacgaat cccgtcgccc agaagtggtc
     4741 ggacctgatg acgcagaatt acgacgccct ctcggccaag gagccggtct tcggcgaatt
     4801 gcggaacctg atggacctgt gcgtgatctc cgctttgatc tcgaaggaag gaatgctgga
     4861 gaaggcgggc ctggaactgc cgacgatcct gggcgagaac agcaagctgg agctggccag
     4921 caactacgct cccaagtcga tcggcacgca gtgcagcttt gtaaagcgcg accgcgagta
     4981 cctgatcacc gcctcgggcg gcgtcgatat cgcctcctgg gaagtcgcca gcaaagccgt
     5041 caccgaaccc tccgtcggcg aaatccgcca aaaagccgcc cccaagaccg gcgggatgtg
     5101 gtggaactaa gcgcttcgta gaacgggttt ccaacccgtc cgcacatcac aggctgctgc
     5161 gtcccaggct cctgcctggg acgcaacttc cccgaggctc tgcctcgttg ccttgctcgt
     5221 tgcctggcgc gccgcgccag gcaaatggct ccgcgttgcg gaatggtctc ccgaccattc
     5281 cgccgcttgg accgaaggtc tcccgcgcgg ctaccttcgc ggctcatagc gcagcgccac
     5341 cgcccccgag ccgaactccc gccggcccac gagcttcaaa tcgacatact tcgacagccc
     5401 cgcgaacaaa gtcggcccat ggcccgccag cctgggatgc accacgaact cgtactcatc
     5461 gatcaatccc agctccgcca gcgcctgcgg gagcttcacg ccgcccacga acaatccctg
     5521 gcccggctcc cgcttgagct gctcgacggc cttacccaaa tctccacgca ccagctccgc
     5581 gttccagtcc acccgttcca gggtgctcga cacaacgtac ttcttcgcca cgtcgatcat
     5641 ccgggcgaag ggttccatcc actcggtcat ccactccggc cgcactccgc tcgccgccgg
     5701 ccgccacgct gcctccatca tttcgtaggt cacccggccg aaaatgagtg catcggcccg
     5761 cgcgaggttc tcggtccagt agcgatgcaa ctcctcgtcg gtcgaacccg cccgatgatc
     5821 gacgcacccg tccaacgtga cgttgatggc ataccgcagc ggtcgcataa cgtgctcctg
     5881 cccaagcggg gcaacctacg attttgacca gcgggcggcg ggcggcgggc ggcggggttt
     5941 tgcgcagcat agcccagcgg gccgccggca agctaccctg actcgatgcg gccgccggga
     6001 agtgtggtgc gccgcggctt ggttctgcgg gcagccgagt ggaagtcgtc cagcgccgcg
     6061 ggattcgcgg aaggcgatag cgtgattccc cttgatccca ctgggccgtc tggcggacgg
     6121 ctgagtcgat cgccacctcg gctgggtcat gctgcgcaag acccagccac ccggccgctt
     6181 tttcccatca ggcaaggtga aacagaagcg aacgaaggca acgaagacac gaatcgggca
     6241 ccgccggtct cacttcagat cgaaatcgac tctgcatgca ccgggcaaaa acgcggcggt
     6301 tacctgggga ttctttgcgt tgccgtactc accaggtagc gaagccggat agtttgccag
     6361 ggctgatctg gcggatcgat tggcatagaa cagcacgaaa tgggcgcccg gtcgaattgg
     6421 ccgaccactg ctgtcatctt tcagacggta gttgccttca aggtccgtta ccgtgctgta
     6481 agctccatcg ccggccgccc taaggaacgt catttggacc aactgtgcgg gccgtcctcg
     6541 aaaggtcact tgcccaaaca ccgcaggcga agtagggcga agactgaata ctgcgattcc
     6601 aatcgccgcg aggaccggcg gccccacggc cagcacgatc agcagtgtgt ggagtcggta
     6661 acgcatcgag cgcatttccg gaggattgaa tgactaggat caacgtccgg cgtcaatccg
     6721 tttcgtctac gaagaatcgc tcgggtatgt tgtagcgctg ccgaaccgcc tcccatgtct
     6781 ttgcttggcg ctctcgttct tcccggagtt cctctaccca aaattgtttc tctgatgccg
     6841 caccttcgag gtaagcctgc tcataactcc cccagtctct ccaccacccg accgccagcg
     6901 ctgccaccac ggtcaaccac aacaagtcgc ggattgtgaa tcggaacata gactacccgc
     6961 ccttccccgt cattcgcggc cagaaggcaa tcaccatcca cagggccgcc agaactgcga
     7021 aacagcctac cgccccagcg taccgccctc ctgtcattgc gtaccagccc aacgctaacg
     7081 ccggcggcgc aaaagcgagc acaactagga gcgttcgaag acggtatcgc atggatagtt
     7141 atagtatccg ccgtttgacg cgcctaatcg ccccggctat cgtaagtcga tacgggcgga
     7201 tggtttgcgg ctgattttgc tgcgggctcc tccgcccctc accctgaccc tctcccacag
     7261 gtggagaggg gaagaaggcc cagcgttgcc gtcggttggc ggtccctcct actgcgtgga
     7321 gcttgtcatg agattctggt cggtcgtcgt gcccttttcg ttcgcgctcg ctgcattgtc
     7381 gcaaatggcg ggctcggccc ccggtcagga gcaggctgcg ggagcgaaga aacgcgtcct
     7441 cttcatcgcc ggcaggccca gccatggcta tggggcgcat gagcacaacg ctgggtgtac
     7501 gctgctggcc aaggaattgc aggcggcaca gccggacatc aagtgcgacg tggtcctgaa
     7561 cggctggccg gcggatagca gcgtgtttga cggggccgat tgcatcgtga tgtactgcga
     7621 cggcggcggc gggcacatgg tcaatccgaa tttggagcag gtcgataagc tggcgaagaa
     7681 gggcgtgggc atcgtctgca tccattacgg cgtcgaagtg cccaaggggc cgagcggcga
     7741 caagtttctc gactggatcg gcggctattt cgagacgaac tggtcggtca atccgcactg
     7801 gactgccaag tacgagaagt ttccggacca tccgatcagc cgcggcgtgc agccgttcga
     7861 gatcaacgac gagtggtatt accacatgcg ttttcgcgag gggatgaagg gggtcacgcc
     7921 aatcctcacc gccctgccgc cgaaggatac gctcagccgt ccggacggca cgcacagcgg
     7981 caatccggcg gttcgcgagg cgatcgccaa aggcgagccg cagcacatgg cctgggccgc
     8041 ggagcgcgaa gggggcggcc gcggcttcgg cttcacgggc gggcacgacc actggaactg
     8101 gggcgatccg aatttccgca aagtgatgct gaacgcgatc gtctggtgcg ctcacggcga
     8161 cgtgccgacc agtggcgtcg aatcgccgac cgtcacgctc agcaccctgg aagtcaatca
     8221 ggacgagccg gcgccggcca atttcgaccg cgaagcgatc cgcaagcgga acaagctgcc
     8281 ggcggatggc gacgccgata gcaaaacgaa tgccgaaggg gccgcagccc gtcagccggg
     8341 cggccggcgc ggtcagcgcg ccactgccgg cggcagcagg gccaagccga tcttttccag
     8401 cccggtcgtt tccgcggcca cgaaagggca tgcggtggag atcgacgccg acatcaccgg
     8461 cgcgaagcga ctgtggctgg tcgtcaccga cggcggcgac ggctttgggt gcgattgggc
     8521 cgattgggcc gagccgcggc tgattggacc gaagggcgag aagaagctga ccgagatttc
     8581 gtggaagtcc gcctccgccg gctttggcaa cgtgaacgtc aataagcgat gtgacggcaa
     8641 ggatatgaag atcaacggcc agagtgtcga atacggcatc ggcacgcatg ccaacagcgt
     8701 gattcagttc gacattcccg agggctacac gcggttcaaa gcccgcggcg gcatcgataa
     8761 cggcgggacc gatcagggct gcggctcgac ggtgcagttc ctcgtctata cgcaggctcc
     8821 gccggcgaat gtcgcctccg tcgccggcgc gaataatccg gtccgcgatg cggagaacgc
     8881 ggtggccaac ctggacgtag ccgacggcct cgaagcgacg cttttcgcca gcgagcccga
     8941 catcagcaac attacgaata tcgatatcga ccacctcggc cgcgtctggg cctgcgaagt
     9001 gaagaactac cgca
//
LOCUS       DASUGB010001117         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_265061, whole genome shotgun sequence.
ACCESSION   DASUGB010001117 DASUGB010000000
VERSION     DASUGB010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265061"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFV87_21275"
     CDS             <1..183
                     /locus_tag="VFV87_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106371.1"
                     /translation="YEDEWTYEQYFETPEQLEEYMEAVLPPDKGRGIRSPKPAGLPLL
                     RTHRQPKVSLPAGTVT"
     gene            183..1520
                     /gene="hisS"
                     /locus_tag="VFV87_21280"
     CDS             183..1520
                     /gene="hisS"
                     /locus_tag="VFV87_21280"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5106372.1"
                     /translation="MIEPRTLKGFRDFLPEAMIPRERLIAAAQEIYRSFGYSPIDTPA
                     LEYHEILVGKGGEESDRQLYQFVDHGGRQVAMRFDLTVPLARFAAQHASEIVLPFKRY
                     HIAPVWRGENTQRGRYREFMQCDFDTVGTRSAAADIETALVIHDLIRKIGSTFGKNFG
                     FTIRLNNRAVLSGLLAKLDLVEKTVPLLRSLDKLGKIGRDRVAAEMVAAAGATPEQAD
                     QVLKLAELSGSNDDILKQLGPLVAGNAIGEEGVSRLRQILAGAKAGGVGEDVLKLDVS
                     IARGLDYYTGAIYETFLADLPTIGSICSGGRYDNLAGLFTKQELPGIGASLGLDRLLA
                     ALDELKIIAPVRTPAPVLIAFFDKDRLEDYVRIAATLRAAGIGVEVFPDPKKLGQQLQ
                     YADRRGFRVAIIAGQREFDAGTCQVKDLLTTQATDVPLADPDQLISAVRKLLG"
     gene            complement(1509..>2169)
                     /locus_tag="VFV87_21285"
     CDS             complement(1509..>2169)
                     /locus_tag="VFV87_21285"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5106373.1"
                     /translation="KRDEITAAVVEYLIKYQADRGHWRTSSNRPPSEASPFTTSYVAL
                     RGLTAFGSEEQQERIAARRDQLREWLVKTPANDNEDRVFRLLALKAVEAEATEIESAV
                     KELLAKQREDGGWSQLDSGEPETATQSDAYATGSALVALHQAGDLCTDDPAWQRGLRY
                     LLKTQKDDGSWHVPSRSKPFQAYFESGFPHGKDQFISCAASGWATWAMILALPESPLA
                     E"
BASE COUNT          368 a          747 c          689 g          365 t
ORIGIN      
        1 tacgaggacg agtggaccta cgagcaatac ttcgagaccc ccgagcagct tgaagagtac
       61 atggaagcgg tcctgccgcc ggacaaaggg cgcggcattc gcagtcccaa acccgccggc
      121 ttgccgctgc tgcgaactca ccgccagccg aaggtgtcac tgcccgcggg aacggtgacg
      181 tgatgatcga gcctcgcacg ctcaaaggct ttcgcgactt tctgcctgag gcgatgatcc
      241 cgcgcgagcg cctcatcgcc gccgcccagg agatctatcg ctcgttcggc tacagcccga
      301 ttgatacgcc ggccctggaa taccacgaaa tcctcgtcgg caaagggggg gaggaatcgg
      361 accggcagct ctatcaattc gtcgatcacg gcggccgaca ggtcgccatg cggttcgacc
      421 tcacggtgcc gctggcccgc ttcgccgccc agcacgccag cgagatcgtg ctccccttca
      481 agcgctatca catcgcgccg gtctggcggg gcgaaaacac gcagcgcggc cgctaccgcg
      541 aattcatgca gtgcgatttc gacacggtcg gcacgcgctc ggccgcagcc gatatcgaaa
      601 cggccctcgt gatccacgac ctgattcgca aaatcggatc gacgttcggc aagaatttcg
      661 gcttcacgat tcgcctcaac aatcgagccg ttttgagcgg cctcctggcg aagctcgacc
      721 tggtcgagaa aaccgtgccg ctgctgcgct cgctcgacaa gctcggaaag atcggccgcg
      781 acagggtcgc tgccgaaatg gtcgctgccg ccggcgctac ccccgaacaa gccgatcagg
      841 tcctcaagct ggccgagctt tccggcagca acgacgacat cctgaagcag ctcggcccac
      901 tcgtggccgg taatgcgatc ggcgaagagg gcgtatcccg cttgcggcag atcctggcgg
      961 gcgcgaaagc cggcggcgtc ggcgaggacg tgctcaagct cgacgtttcg atcgcccgcg
     1021 gcttggacta ctacaccggc gcgatttacg agacgtttct ggccgacctg ccgacgatcg
     1081 gctcgatctg ctcaggcggc cggtacgaca atctggccgg cctgttcacg aagcaagagc
     1141 tgcccggcat cggcgcctcg ctcggcctcg accgcctgct cgccgccctc gacgagctga
     1201 agatcatcgc gccggtccgc acgccggcgc ccgtgctgat cgccttcttc gacaaagacc
     1261 ggctggagga ctacgtgcgg atcgccgcga cgctccgcgc cgcgggcatc ggcgtcgagg
     1321 tcttccccga tcccaaaaag ctcggccagc agctccaata tgccgaccgc cgcggcttcc
     1381 gcgtggccat catcgccggc cagcgcgaat tcgacgccgg cacctgtcag gtgaaagacc
     1441 tgctcaccac ccaggccacc gacgtgccgc tcgccgaccc cgatcagctc atctcggcgg
     1501 tccgcaagct actcggctag aggcgactcc ggaagcgcaa ggatcatcgc ccacgtcgcc
     1561 cagccgctgg cggcgcagga gatgaactgg tccttcccgt gcgggaagcc gctctcgaaa
     1621 taggcctgaa acggcttgct gcgggacggg acatgccacg agccgtcgtc tttttgtgtc
     1681 ttcagcaggt agcggagtcc gcgctgccag gcggggtcgt cggtgcaaag gtcgcccgcc
     1741 tggtgcaggg cgaccagggc cgagccggtg gcgtaggcgt ccgattgcgt ggccgtttcc
     1801 ggctcgcccg aatcgagctg cgaccagccg ccgtcttcac gctgcttggc gagcagctct
     1861 ttgaccgccg attcgatttc ggtggcttcg gcctcgacgg ctttcagcgc cagcagccgg
     1921 aacacgcggt cctcgttgtc gttggccggc gtcttcacca gccactcgcg aagctggtcg
     1981 cggcgggcgg cgatccgctc ctgttgctcc tcactgccaa aggcggtcag gccgcggagg
     2041 gcgacgtagc tcgtcgtgaa cgggctggcc tcggacggcg ggcggttgga cgacgtccgc
     2101 cagtggcccc ggtcggcctg gtacttgatc aggtactcga ccacggcggc ggtgatctcg
     2161 tctctcttg
//
LOCUS       DASUGB010001118         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_680636, whole genome shotgun sequence.
ACCESSION   DASUGB010001118 DASUGB010000000
VERSION     DASUGB010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680636"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VFV87_21290"
     CDS             complement(<1..1295)
                     /locus_tag="VFV87_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106374.1"
                     /translation="MEARFLRAAALAAVSTLVFSVCSSTSQAQFARQHSSVRSEHFIV
                     SAPSRELAEEICQAAEKYRKDLAIEWLGKELGPWREPCPIQAHVHPQLGAGGVTSFMF
                     DRGQPWGWTMTIQGSRERILDSVLPHEVTHTIFATHFGRPLPRWADEGACTTVESPVE
                     KSKQDKFLIQFLTTDRGIPFNQMFAMKEYPADILPLYSQGYSLARYLIHQGGKRKFVD
                     YVGEGMQTNNWTATTKKHYGFTSLSDLQVTWVEWVRQGSPDISALASAAVADVPTGET
                     PTRSVSEGVTAIPTRSASEGFSPSNLPGQIADTQMASMTRIRSAGAGPRQTKPWQSVG
                     AQRERSIATAQHFEPPPQPSSFPAESYVTNPQYSSDHSSVSRPVSEGWYAKRRDQAQA
                     VLHTSPEAPTPTGGTIPAPPTRASASEALTSAAAFSPSPP"
     gene            1849..>3214
                     /gene="dnaG"
                     /locus_tag="VFV87_21295"
     CDS             1849..>3214
                     /gene="dnaG"
                     /locus_tag="VFV87_21295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5106375.1"
                     /translation="MTYGDAKEQVRQATDIVELVGKHLDLRRAGRGYVGRCPWHDDRK
                     PSLQVNPDRQTWKCWVCDIGGDVFSFVMKREGCDFREALQMLADRAGINLDPGGKKKK
                     IEPGSPDDKNTLYQCCEWAARQFHEFLLRSDGAEIARKYVEERNITPMSVERFRLGFV
                     PNENGWLQSRARSTPFTPAVLEAAGLLSKYEDSGNYRDFFRGRVMFPIRDTQSRVVAF
                     GGRILPQFATDNTGKYINSRETRLYTKSDALYALDIARIATQKSRQLTIVEGYTDVIL
                     CHQHGDNDVVACCGTAVNERHISLIKRFADTVYLVLDGDVAGQNSANRLLEIFVAAQL
                     DLRLLTLPDELDPAEFMEERGGDAFRELLKGAIDALEHKIRHVTRGIDLARDTHRANQ
                     ALEEILRTIAAGLPAGTLDQAGLRAQQLLARLARQFVLDEADVRGRFNQLRPGMKSKY
                     GDLSQ"
BASE COUNT          604 a         1018 c         1006 g          586 t
ORIGIN      
        1 ggcggcgagg gagagaatgc ggcagcagag gtgagggctt cgctggcgga ggctcgcgtc
       61 ggtggcgcag gaatcgtccc gcctgttggg gtgggcgctt ctggcgaggt atgcagcacg
      121 gcttgcgcct ggtcgcggcg cttggcatac caaccttcgg aaacgggccg cgagacgctg
      181 ctgtggtcgc tggagtactg cggattggtc acgtacgact cggccggaaa gctcgacggc
      241 tgcggcggcg gctcgaagtg ctgggccgtt gcgatgctcc gctcgcgctg agcacccacc
      301 gattgccaag gcttcgtctg gcgcggcccg gcgcccgccg atcgaatgcg ggtcatggac
      361 gccatctgcg tatcagcgat ctggccaggc agattggacg gcgagaatcc ctcgctggcg
      421 ctgcgggttg gtatagccgt gactccctcg ctgacgcttc gggttggcgt ttcacccgtc
      481 ggcacatcag ccacagcggc cgaagccaac gccgagatat cggggctgcc ctgccgcacc
      541 cattcgaccc aggtgacttg caggtccgac aggctcgtga agccatagtg cttcttggtc
      601 gtggcggtcc agttgttggt ctgcatcccc tcgccgacat aatcgacgaa cttccgcttg
      661 cctccctgat gaatcaagta acgggccagc gaatagcctt gcgaatacag gggcaggatg
      721 tcggcagggt actccttcat cgcgaacatc tggttgaacg gaatgccgcg gtcggtggta
      781 agaaactgga tcagaaactt gtcctgcttg gatttctcga ccgggctttc gaccgtcgtg
      841 caggcccctt cgtcggccca ccgcggcagc gggcgaccaa agtgtgtggc gaaaatcgtg
      901 tgcgtgacct cgtgcggcaa gacgctgtcg agaatccgct cgcgcgaacc ctggatcgtc
      961 atcgtccagc cccagggctg gccacggtcg aacatgaagc tcgtcacgcc ccccgcccca
     1021 agctgcgggt gcacgtgggc ctgaatcggg cacggctcgc gccacgggcc tagctccttg
     1081 cccagccact cgatagccaa gtctttgcga tacttctcgg cggcctggca gatttcctcg
     1141 gccagctcac ggctgggtgc ggagacgatg aagtgctcgg aacgaacgct ggaatgctga
     1201 cgggcaaact gcgcttggct ggtgctgctg caaacgctga agacgagcgt gctgacggcg
     1261 gcaagcgctg ccgcacgcag aaatcgagct tccatgctgc tgggtccttc cgtggaacgt
     1321 gggccggccg gctcctgtcc ggcggcatct ggcgaatcca ctcgcgtctg cttcgggaaa
     1381 gcaaaccgcg agccgaagcc gcgccgcgac ttcgccgcct ccctccttgg agcggcgata
     1441 agtcgttttg cggcttcgcc ttacccgttg acgtcgaact gtgctgcgcg agcgaggatc
     1501 gacggactcg ttgtaagagt tgcaaaccct agtcgatcac ctgcaattca ccaagagcga
     1561 atccgacgcg attgtgacac tcatatttcg ccgcggtgct cgcaccgcga tcgcaccccc
     1621 acggtgagta ccgcaactgg aaacgcgcgg tgcaagcacc gcggcgaaat gtacaacgac
     1681 acgcaaactg acgttgtctt gcggccccgc gtggacttat catttccgcc cgtgctaagg
     1741 agggcacact gccgactcgc cgaccccgcg agacggcagg cgcgttatac tccggcaatt
     1801 ggcctgttgg accattcccg caggccaggc caattgagga gtcgcagcgt gacgtacgga
     1861 gacgccaagg agcaggtccg ccaggcgacg gatatcgtcg agctggtcgg caagcatctc
     1921 gacctgcgcc gggccggccg cggttacgtt ggccgctgcc cttggcacga cgaccgcaag
     1981 cccagcctgc aggtcaaccc cgaccgccag acctggaagt gctgggtctg cgacatcggc
     2041 ggcgatgtct tcagcttcgt gatgaagcgc gagggctgcg atttccgcga agccctgcag
     2101 atgctggccg accgggccgg catcaatctc gatccgggcg gaaaaaaaaa gaagattgag
     2161 cctggcagcc ccgatgacaa gaacacgctt taccagtgct gcgagtgggc cgctaggcaa
     2221 tttcacgaat tcctgctgcg gagcgatggg gcagaaatcg cccgcaaata tgtcgaggag
     2281 cggaacatca cgccgatgag cgtggaacgg ttccggctcg gtttcgtccc caacgaaaat
     2341 ggctggctcc agtcccgcgc tcgctccacg ccgtttactc ccgccgtttt ggaagccgcc
     2401 gggctgcttt cgaaatacga agacagcggc aactatcgcg actttttccg cggtcgcgta
     2461 atgtttccga ttcgtgatac gcagagccgc gtcgtcgcct tcggtgggcg cattctgcct
     2521 caattcgcca ccgataatac gggcaagtac atcaactccc gtgaaacgcg gctgtacacg
     2581 aaaagcgacg ctctatacgc tctggacatt gcccgaattg cgacgcaaaa atcgcggcaa
     2641 cttaccatcg tcgaaggtta cacggacgtc atcctgtgtc atcagcacgg cgacaacgat
     2701 gttgtagcct gctgcggcac cgccgttaat gaacgacata tcagtctgat taagcggttc
     2761 gccgacacgg tctatctggt gctcgacggc gacgttgccg ggcagaacag cgccaatcgc
     2821 ctgctagaaa ttttcgtcgc ggcccagctt gacctgcggc tgctgacgct gcccgacgag
     2881 ctggacccgg cggagttcat ggaggagcgc ggcggtgatg cgtttagaga attgctcaag
     2941 ggcgcgatcg acgccctgga gcacaagatt cgccacgtca cgcgcggcat cgacttggcc
     3001 cgcgacaccc accgcgccaa ccaggccctg gaagaaatcc ttcgcacgat cgctgccggc
     3061 ttgcctgccg gcacgctcga ccaggccggc ctccgcgccc agcagcttct ggcccgcctc
     3121 gcccgccaat tcgtcctcga tgaagccgac gtccgcggcc ggttcaatca actgcggccg
     3181 ggcatgaaga gcaaatatgg cgacctcagc cagc
//
LOCUS       DASUGB010001119         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1375835, whole genome shotgun sequence.
ACCESSION   DASUGB010001119 DASUGB010000000
VERSION     DASUGB010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375835"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFV87_21300"
     CDS             complement(<1..784)
                     /locus_tag="VFV87_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106376.1"
                     /translation="MGFRIADCGLRIAEGLFAAAILCAVPVIALAEETEAERAERLKN
                     MTLEQKDAVLNKQDRFEKLSAEEQERLRALHVSIESAPNGTELRQTLSRYHAWLATLT
                     SIQRSELLTLPADERIVRIKELLKQQESQRFQAFVGNLPPEDHTAIFNWLGEFVAEHE
                     AEILAQLDFSERGRVRNAPDREAKNRLLALYMAAKRQNPKMPMPSVDDLNRLMERVST
                     EATRQIEQAPADQQPERIREMVRAAIFSRRLPPVNDEELQKFY"
     gene            complement(774..1283)
                     /locus_tag="VFV87_21305"
     CDS             complement(774..1283)
                     /locus_tag="VFV87_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106377.1"
                     /translation="MNEPDNTVATAATIDEELVAYLDGELDASGAARIERRLADEPSY
                     RARLAQLERAWDMLDSLHRVEAHDDFARSTVEVIAVKAIDDAKTGQMRAIRRRNLGWL
                     ATSLAVIAAAVGGYLLFSYKLQQPNRELVRDLPVIERIDEYRNADSVEFLQRLHDEGL
                     FAAEVEDGI"
     gene            complement(1395..>2033)
                     /locus_tag="VFV87_21310"
     CDS             complement(1395..>2033)
                     /locus_tag="VFV87_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5106378.1"
                     /translation="RLMLQVRSGSAPAFEELVLRYQNRLLTVLEHLVGNREQAEDLAQ
                     EVFLRVFRARERYEPGAKFSTWLFTIANNVASNALRSRSRRREVGVPEGNGSDSSALQ
                     LDQLAKAASGFMPTRALDKAEQAEMVRHAVSALSERQRMALLLAKFEGMSYQDIAQSM
                     DLSVQAIKSLLSRARVNLKEILTPYIEQGTRLDQARPAAAADEEETEDQDEE"
BASE COUNT          337 a          685 c          609 g          402 t
ORIGIN      
        1 tgtagaactt ctgcaattct tcgtcattca cgggcggcag gcggcggctg aaaatggcgg
       61 cccggaccat ttcgcgaatg cgttccggct gctggtcggc cggggcttgc tcgatttgcc
      121 gtgtggcttc cgtactcact ctttccatca ggcggttcaa atcatccaca cttggcatgg
      181 gcatttttgg attctggcgc ttggcggcca tatatagggc gagcaggcga ttcttcgcct
      241 cccggtcggg agcgtttcgc acgcgcccgc gttcgctgaa atcgagctgt gccaggatct
      301 ctgcctcatg ctcggcgaca aactctccca gccagttgaa aatggccgtg tggtcttcag
      361 gcggcaaatt gccgacaaac gcctggaacc gctggctctc ctgctgcttg agcagctcct
      421 tgatccgcac gatgcgctcg tcggccggca acgtgagcag ctcggaacgc tggatagatg
      481 tcagcgtcgc cagccaggcg tgatagcgcg acagcgtctg ccgcagctcc gtgccgttgg
      541 gcgccgactc aatcgaaacg tgcagtgcgc ggagccgctc ttgttcctcc gcggacagct
      601 tctcaaagcg gtcctgcttg ttgagcacgg catccttctg ctccagcgtc atgtttttca
      661 gccgctcggc ccgctcggct tccgtctctt cggccagagc aatcacgggg accgcgcaga
      721 gaatcgccgc ggcaaacagc ccctccgcaa tccgcagtcc gcaatccgca atcctaaatc
      781 ccatcttcca cctccgccgc aaacaggccc tcgtcgtgca gccgctgcaa gaactccacg
      841 ctgtcggcgt tgcgatattc gtcgatccgc tcgatcaccg gcaagtcacg gaccagctcg
      901 cggttcggct gctggagctt gtaagagaac aggagatacc cgcccaccgc agccgcgatg
      961 accgccagcg aagttgccag ccagccgaga ttccggcggc ggatggcccg catttggccg
     1021 gtcttggcgt cgtcgatcgc cttgaccgca atcacctcga cggtgctgcg ggcgaaatcg
     1081 tcgtgagcct cgacgcggtg cagcgaatcg agcatgtccc aggcccgctc gagctgggcg
     1141 aggcgggcgc gatacgaggg ttcgtcggcc agccgccgct cgatccgggc ggcgccggag
     1201 gcatcgagct cgccgtcgag ataggcaacg agctcctcgt cgattgtcgc ggcagtggca
     1261 accgtgttat cgggttcgtt catggcgtat acaaacctgt cagtctgcct ggtagccgcg
     1321 ttcgccagaa cgcgggaatg ggcgccgtat tgcccgcact ctggcgaatg cggctacgat
     1381 ttcgaaactg ccagtcactc ctcatcctgg tcctctgttt cctcttcatc ggccgccgca
     1441 gcgggccgcg cttggtccag cctcgttcct tgttcgatat agggcgtcaa aatctctttc
     1501 aaattcacac gggcccgcga gagcagcgac ttgatcgcct gcacgctcag gtccatcgac
     1561 tgggcgatgt cctggtagct catcccttca aacttcgcca gcagcagggc catccgctgc
     1621 cgctcgctca gggccgagac ggcatggcgg accatctcgg cctgctccgc cttgtccagg
     1681 gcccgcgtcg gcataaagcc gctggcggct ttggcgagct ggtcgagctg caaggcgctg
     1741 ctgtccgatc cgttcccctc cggcacgccc acttcgcgcc gccgcgaacg gctgcggagg
     1801 gcgttgctgg ccacgttgtt cgcaatggtg aacagccacg tgctgaactt cgcccccggc
     1861 tcgtaccgct cgcgagcacg aaaaacccgc aggaacacct cctgggccag gtcctcggct
     1921 tgctcccgat tgccgaccaa atgctccaac accgtcagca ggcggttctg gtagcgcagc
     1981 acgagctcct cgaaggccgg cgcgctgccg ctgcgaacct gcagcatcag gcg
//
LOCUS       DASUGB010001120         5534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_680875, whole genome shotgun sequence.
ACCESSION   DASUGB010001120 DASUGB010000000
VERSION     DASUGB010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680875"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1744
                     /locus_tag="VFV87_21315"
     CDS             119..1744
                     /locus_tag="VFV87_21315"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5106379.1"
                     /translation="MLRNVLHDAMVWIGVAIGLGFQLPARAADADPTKKDAHETAAEI
                     DRRLEAHWQAKGITPAAAASEVTVFRRLMLDLAGRIPTRAECEAYLAEKAAPEERYRQ
                     AVQRILAGPEFALHQGAVLEEMLQGDSAGNPEFTDYLRRSLRERKGWDALFRELLVGP
                     WKTDDLKPATRFLEVRAKDLDRLTTDATRVFFGVDVSCARCHDHPLVDDWKQDHYYGM
                     ASFFNRTSGGKGSIGEKFEGDVSFKSIDGTQKTAALMFLSGRVATEPPPEEAKKTKFS
                     RREQLVAIALEDKAFLSRSLVNRLWQQYFGRGLVHPVDQIHSSNPPSVPGLLEYLADD
                     FADSGYDLQRLIGAIVSTKAYRLSSRWDSAAPLPEEGDFAASRLRLLSPRQYAMSLLV
                     ATGRAEFGPADALQARAEKLAGSEGVGRVAQYLAAEEQARPLFSQIDPTEGGQSSADE
                     ALYLSNSAAIQTLVSAEEGTLAARLAEMSDDRSIAEAAIRHVYCRPPDEGEIERLAGW
                     LDEQSQAPSFEKVRTCEQIVWILVTSAEFRFQH"
     gene            1760..3058
                     /locus_tag="VFV87_21320"
     CDS             1760..3058
                     /locus_tag="VFV87_21320"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106380.1"
                     /translation="MSRHSACTPAEHALHRRTFLGGLLAGGAAALSLPGVSLADDLPA
                     AADLKQQNKRALFIWLAGGASQFEMWDPKPGRDTGGPFRTVQTAVPGYHVCELMPKLA
                     ARMNKLAVVRSLNTQISEHGQAADLMSTGWPKEPALAHPEIGVVLAKELALQESLLPD
                     YVSLFTTTEGRRRPETGFLSRRHGALLLEDSLEPGNIDLPAGLTSDQHAAREDLRAAL
                     SRRFADRRAGSDLVEGYNAAYARVRGLMRSDRLFDLDQEPAVARGRYGKTPFGEHCLL
                     ARRLLEAGVPMVKVARGFWDSHHDNFESHRELVPDFDHVLSALLDDLEERGMLSSTLV
                     FVLSEFGRTPKINQDVGRDHYADAWSLAAAGCGIRGGAVYGKTDDDGVKVVEGETSAG
                     DVAATIFQAVGIDPAKHYQVGQRPVPLAKEDARPIEAILA"
     gene            3055..4074
                     /locus_tag="VFV87_21325"
     CDS             3055..4074
                     /locus_tag="VFV87_21325"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106381.1"
                     /translation="MTTFDPKQAALLHTAEAPLFFASCFDEASGMYYGAGIDAAVYSL
                     DTRADKPAAERRWKHHDNYVSALAWRDGTVISSGYDRRIVWTQAESGEKLREVTAHDG
                     WIRDMVLFSGGQQLATVADDMLVKLWDATTGELVRSFDGHERQTPEGFATAVYAIAAS
                     SDGKLIASGDRIGHVCLWEVETGKLLVRLKAPEFYTYDRRTRDRSIGGIRALLFLPDG
                     RLAIGGLGPVSNVDGFVGPARFEIWDWQAAKLLQRCQDKHHAILDHLQFATEQNLMIG
                     GGGGDAGPFLGFWDIASEAPIHKAKPKDHIHHFIFDGAKSRLLAVGAGGFQMWEFSGK
                     QSEGR"
     gene            complement(4131..4565)
                     /locus_tag="VFV87_21330"
     CDS             complement(4131..4565)
                     /locus_tag="VFV87_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106382.1"
                     /translation="MTLLAVACLLGVGCGDGGPKLNRVSGKITFKGAPVPAGTVYIRP
                     DTAQGNSGPAGIAYIKDGVYDTSSSGGKGSVSGPVVMSVDGLDPTPPPNAGPDVTVTS
                     LFSSYETKAEIKAPETVHDIDVPPEAAQVPSSGETTGGAIVP"
     gene            complement(4801..>5534)
                     /locus_tag="VFV87_21335"
     CDS             complement(4801..>5534)
                     /locus_tag="VFV87_21335"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5106383.1"
                     /translation="WIVHILPFIEQKNIYDLYRFDLPYNDTTNGTLGNTVIQTLYCPS
                     GPDPQKYLDGNTNTASNPTTHYYGIMGPGGPADNFQIVVSGVTYQYRYGSAASNGSWS
                     YHGILSHFINNPGSTSISANRVVRMADVLDGTANTLMLGEISKTLPILNRSTGTTQAN
                     QYRTWIRGNNGGSGATKNVRYPINSTFYTSNNFNEISFGSDHAGAGALFANADASVRF
                     IRENVDLTTYIMFSSMNGNESVLLP"
BASE COUNT         1046 a         1659 c         1805 g         1024 t
ORIGIN      
        1 aaccagagat tccggccggg gaaaggcggt atccgcaaca tcaacgcgcg ttgatatccg
       61 tcaaagggtg tgttatctgt tacaggtaac tctggaaacc aggcggggaa ctcgacctgt
      121 gcttcgcaac gtgcttcatg atgcgatggt ttggatcggc gtggcgattg ggttgggatt
      181 tcagcttcca gcccgtgccg ccgacgcaga tccgaccaag aaagatgcac acgagacggc
      241 tgccgagatc gatcggcggc tggaggcgca ctggcaggcg aaaggcatca cgcccgcggc
      301 ggcggcaagc gaggtgaccg tctttcgccg gttgatgctc gatctggcgg ggcggattcc
      361 gacgcgggca gagtgcgaag cctatctggc cgaaaaggct gctcccgagg agcgctatcg
      421 gcaagccgtg cagcgaatcc tcgcggggcc cgagtttgcc ctgcaccagg gtgctgtgct
      481 ggaggaaatg ctgcaaggcg actcggccgg caatccggag ttcaccgatt acctgcggcg
      541 cagcctgcgc gagcgaaagg gttgggacgc cctgtttcgc gaactgctgg ttggcccgtg
      601 gaagaccgac gacctcaagc cggccacgcg ctttctggag gtgcgggcca aggacctcga
      661 tcggctgacg accgatgcga cgcgcgtatt cttcggcgtc gatgtctcgt gcgcccgctg
      721 ccacgatcat ccgctggtcg atgactggaa gcaggaccac tactacggca tggcgtcgtt
      781 cttcaaccgc acgtccggcg gcaagggatc gatcggcgaa aaattcgaag gggacgtgag
      841 cttcaagagc atcgatggca cgcagaagac ggccgcgctc atgtttcttt ccggccgcgt
      901 ggccactgaa ccgccccccg aggaagccaa gaagaccaaa ttcagccgcc gcgagcaact
      961 tgtcgccatc gccctggaag acaaggcctt ccttagccgt tcactcgtga accggctgtg
     1021 gcagcagtat tttggccggg ggctggtgca tccggtcgat cagattcact cgagcaatcc
     1081 gccgagcgtg ccgggtctcc tggagtacct cgccgatgac ttcgccgact cgggctacga
     1141 tcttcagcgc ctgatcgggg cgatcgtctc gaccaaggcc tatcgacttt ccagccgctg
     1201 ggattcggcc gcgccgttgc cggaggaagg cgatttcgcc gccagccgtc tgcggctgct
     1261 ctcgccgcgg cagtatgcga tgtcgctgct ggtggcgacg ggccgggccg aatttggacc
     1321 ggcggatgca ttgcaagcgc gggccgaaaa actcgccggc tccgaaggcg tcggtcgcgt
     1381 cgcccagtac ctggctgccg aagagcaggc cagaccgctc ttttcgcaga tcgatccgac
     1441 cgagggaggc cagtcgagcg ccgacgaggc gctgtatctt tccaatagcg cggcgattca
     1501 gacgctggtg agcgccgaag agggcacgct cgccgcccgg ctggccgaga tgagcgacga
     1561 tcgatcgatc gcggaggccg cgattcgcca cgtctattgc cgcccgccgg atgaaggcga
     1621 gatcgagcgg ctggcgggat ggctcgatga gcagagtcaa gccccgtcct tcgagaaggt
     1681 ccgcacctgc gagcagatcg tatggattct cgtgacgtca gctgagtttc gtttccagca
     1741 ttaaatgtga ggcggtacca tgtcgcgcca ctccgcttgc acgcccgccg aacacgccct
     1801 ccaccgccgc acgtttctcg gcggcctctt ggcgggcggg gcggcggcgc tgtcgctgcc
     1861 gggcgtttcg ctggcagatg accttcccgc tgccgctgac ctcaagcagc agaacaagcg
     1921 ggccctgttc atctggctgg ccggcggcgc gagccagttc gaaatgtggg accccaagcc
     1981 gggtcgcgac accggcggac catttcgcac cgttcagacg gcggttcccg gctatcacgt
     2041 ctgcgagctg atgccaaagc tcgccgcgcg aatgaacaag ctggcagtcg tccgctcgct
     2101 gaacacgcag atctccgagc acggtcaggc ggccgacctg atgagcaccg gctggccgaa
     2161 ggagcccgcg ctggcgcatc cggagattgg cgtggtccta gccaaggaat tggcccttca
     2221 agagagcctg ctgccggact atgtctcgct ctttacgacc accgagggtc gtcggcggcc
     2281 ggaaaccggg ttcctgagcc ggcggcacgg cgcgctgctt ttggaagact cgctggagcc
     2341 agggaatatc gatctgcccg ccggactgac gagcgaccag cacgctgctc gcgaggattt
     2401 gcgggcggcc ctcagcaggc gctttgccga ccggcgtgcc ggcagcgacc tcgtggaagg
     2461 gtacaacgcg gcgtatgcgc gcgtccgcgg cttgatgcga agcgaccggc tcttcgatct
     2521 cgatcaggag ccggcagtgg cccgcggtcg gtacggcaag acacccttcg gcgaacattg
     2581 cctgctcgcc cggcgactgc tggaagcggg cgtgccgatg gtcaaagtgg cccgcggctt
     2641 ctgggacagc catcacgata acttcgagag ccaccgcgaa ctggttccgg atttcgacca
     2701 tgtgctgagc gcgctgctcg acgatctgga agagcgcggg atgctctctt ccacgctggt
     2761 gttcgtcctg tcggagttcg gccgcacgcc gaagatcaat caggacgttg gccgcgacca
     2821 ctatgccgac gcctggtcgc tggccgccgc gggctgcggc attcgcggtg gcgcggtgta
     2881 tggcaagacc gacgacgatg gagtcaaggt cgtggagggt gagaccagcg ccggcgacgt
     2941 tgccgcgacg atcttccagg cggtcggcat cgacccggcc aagcactatc aggtcggcca
     3001 gcgcccggtg cctctcgcga aggaagacgc ccggccgatc gaggcgatcc tcgcatgacg
     3061 accttcgatc ccaagcaagc cgcactcctg cacacggccg aagcgccgct gttctttgct
     3121 tcgtgcttcg acgaagccag cggaatgtat tacggcgccg gcatcgatgc tgccgtctat
     3181 tcgctcgata cccgagccga caagccggct gccgagaggc gatggaagca tcacgacaac
     3241 tatgtctccg ccttggcgtg gcgggacggc acggtgatct catctggcta cgaccgccga
     3301 atagtgtgga cgcaagccga atcgggcgag aagctccgcg aagtgacggc gcacgacggc
     3361 tggattcgcg acatggtctt gttttctggc gggcagcagc ttgcgacggt ggccgacgac
     3421 atgctcgtca aactgtggga cgcgacgacc ggtgaattgg tgcgatcgtt cgacggccac
     3481 gagcgccaga cccccgaagg ttttgctact gcggtctacg ccattgccgc cagctcggac
     3541 ggcaagctga ttgccagcgg ggaccggatc ggccacgtct gcctgtggga ggtcgagacg
     3601 ggcaagctac tcgtgcgcct caaggctccg gagttttaca cctacgaccg ccgcacgcgc
     3661 gaccgctcca ttggcggcat ccgggcgctg ctctttctgc ccgacggccg gctggcgatc
     3721 ggcggattag ggccggtgag caacgtcgat ggcttcgtgg gccccgcgcg gtttgaaatc
     3781 tgggactggc aagctgcaaa gctgctccag cgctgccagg acaagcatca tgcgatcctc
     3841 gaccacctgc agttcgcgac cgagcagaac ctgatgatcg gcggcggagg aggagacgcg
     3901 ggacccttcc tcggcttttg ggacatcgcg tctgaggccc caattcacaa ggcgaagccc
     3961 aaggaccaca tccatcactt tatattcgat ggcgcaaagt cgcgattgct ggccgttggc
     4021 gccggtggat ttcagatgtg ggaattcagc ggcaaacaaa gcgaagggcg ctgacgtgct
     4081 tcggtcgccg tccgcaactg ctcgcagacc acgcggctga gattgcgtcg tcaggggaca
     4141 atggcgccgc ctgtggtttc tcctgaggaa ggaacctgag ccgcctcggg aggaacatcg
     4201 atgtcgtgaa cggtttccgg cgctttgatt tcggccttcg tctcgtagct ggaaaagagt
     4261 gacgtgacgg tgacgtctgg gccagcatta ggaggcggag tcgggtcgag accatccacg
     4321 gacatcacga caggccccga aaccgaacct ttgcccccgg aggaggacgt gtcataaact
     4381 ccgtccttga tataggcgat gcccgccgga ccgctgttgc cttgtgccgt atcgggccgg
     4441 atgtacacgg tgcccgcggg gaccggagcg cccttgaatg tgatctttcc cgagacgcgg
     4501 ttcagcttcg gccctccgtc gccgcagccg acgccgagga ggcaggcaac cgcaagcagc
     4561 gtcacgggga caaggaaacg aaaagatcgt tgacaggtca tggcaggaaa actccccatc
     4621 ttacgaacgc cgggaacgat tgttggcatg agactgaacg ggcgaggcga cagattttcc
     4681 ccgcctgatt tgtccaaaac tcatcgattc atcatcagcc gttatagcgt accaaagcgt
     4741 aaacgaaaaa gcccgccctc gaaccggggc gggcaaaatt cttcgttaaa ccggagaagc
     4801 ctacgggaga aggacgcttt cgttcccgtt catgctggag aacatgatgt aggtggtcaa
     4861 gtccacgttc tctcgaataa atcgcacgga tgcgtcggcg ttcgcgaaga gcgccccggc
     4921 gccggcgtgg tcgctgccga agctaatctc gttgaaatta ttcgacgtat agaacgtgga
     4981 gttgatggga tacctgacgt tcttggtcgc gccactgccg ccgttgttac ctcgaatcca
     5041 ggttcggtac tgattggcct gcgtcgtacc ggtggatcgg ttcaaaatcg gcaaggtctt
     5101 ggagatttcg cccaacatca aggtgttggc cgtgccatcg agcacatccg ccatgcgcac
     5161 aacacggttc gcactgatgc tggtgctgcc tgggttgttg atgaaatggc tcaagatacc
     5221 gtggtaggac caagacccgt tgctggcagc ggacccatac cggtattggt aggtgactcc
     5281 gctcacgacg atctgaaagt tatcagctgg accgccgggc cccatgattc cgtaatagtg
     5341 cgtcgtcggg ttggacgcgg tattcgtgtt cccatcgagg tacttttggg gatcaggacc
     5401 ggatggacaa tacaatgtct ggataaccgt attgccgagc gtgccgttgg tcgtgtcgtt
     5461 gtagggcaaa tcgaatcggt agagatcgta aatattcttc tgttcgatga agggaaggat
     5521 gtggacgatc caac
//
LOCUS       DASUGB010001121         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1376096, whole genome shotgun sequence.
ACCESSION   DASUGB010001121 DASUGB010000000
VERSION     DASUGB010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376096"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="dprA"
                     /locus_tag="VFV87_21340"
     CDS             complement(<1..825)
                     /gene="dprA"
                     /locus_tag="VFV87_21340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5106384.1"
                     /translation="MSPVPESESGPDDAAAELLLSLVPGIGPRLRKTLVEHFGSAAAV
                     LAASASDLRNVPGIGTKNGRAIVAARQEIDVAAELDRCREQGVQLVTQTHPDYPQPLK
                     SIFDPPGVLFVRGTLAPTDGLAVAIVGSRHGTPYGITQAERLAAGLARAGYTIVSGLA
                     RGIDAAAHRGALKAGGRTLAVLGSGVLNVYPPEHLRLADEIIAQGAVLSENPPLSPPL
                     PGAFPQRNRIVTGLSLGVIVVEASDRSGALISARHAAEQDREVFAVPGRVDSRMSRG"
     gene            complement(1148..1777)
                     /locus_tag="VFV87_21345"
     CDS             complement(1148..1777)
                     /locus_tag="VFV87_21345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5106385.1"
                     /translation="MDRDPIVFVVDDDQAARESVVALVESKGLRVQGFASAEEFLDQY
                     DPSEVGCLVVDVRMNGMTGLQLIEQLATQGSKLPVVVITGYGDIPMAVRAMQMGAVTF
                     LQKPCQVDELWEGIRKALEQEYVRQVGSSQRSEVQARSANLSSEELAVFHKILEGLPN
                     KRIATDLDIGLRTVEMRRANIMRKMQADSLPQLVRMAIVGGFLKPELPT"
     gene            complement(2054..3139)
                     /gene="aroB"
                     /locus_tag="VFV87_21350"
     CDS             complement(2054..3139)
                     /gene="aroB"
                     /locus_tag="VFV87_21350"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5106386.1"
                     /translation="MATTIRVALGERGYDIEIGSGTLAGAGQFVSGRLPGCKQAAIIT
                     DENVGPRHAVPIERALAAAGVKCDVVTVPAGEASKSVGQAELLWNELVRRQADRKTAV
                     VAVGGGVVGDLAGLVAATFARGLSLVQIPTSLLAQVDSSVGGKVGINLPAAKNIVGAF
                     WQPAGVLIDLDVLSTLPEREYRSGLAEVVKYGVILDAAFFEYLEKQADKLIARQPEVL
                     EHVIARSCRLKASVVERDEREETGLRAVLNYGHTFCHAIETVTGYRQYLHGEAVSIGM
                     VCAARLAESLGRIDGSLGLRQQALLEKLGLPVAIEGLDRDELLAAMRRDKKAEHGRLR
                     LVLPARLGQVELVSAVDEDLVRRAWSD"
     gene            complement(3229..3453)
                     /locus_tag="VFV87_21355"
     CDS             complement(3229..3453)
                     /locus_tag="VFV87_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106387.1"
                     /translation="MADKFDPYREALVMETNTIWPEAYEEMPPVEKARLEAKLHADPA
                     ACAGLEYVRTHTGFCRQITVSDADVQRVSG"
     gene            3678..4472
                     /locus_tag="VFV87_21360"
     CDS             3678..4472
                     /locus_tag="VFV87_21360"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5106388.1"
                     /translation="MQAYLDLLRHILDRGTPKSDRTGTGTKSVFGYQMRFDLAAGFPL
                     LTTKKLHLKSILYELLWFLRGETNIAWLKERGVSIWDEWADEFGELGPVYGYQWRSWP
                     APDGRKIDQITDVIQRIQTTPDSRRLIVSAWNVADLDRMALPPCHLLFQLYVAEGRLS
                     CQMYQRSADVFLGVPFNIASYALLTRMVAQVTGLAPGEFIHTFGDAHLYTNHFEQARL
                     QLTREPRPLPTLRINPEVRSIFDFRYEDFRLENYDPHPHIPAPVAV"
     gene            complement(4596..>5185)
                     /locus_tag="VFV87_21365"
     CDS             complement(4596..>5185)
                     /locus_tag="VFV87_21365"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5106389.1"
                     /translation="SNESAEDGTRSVPATLHPWNLVQSFQAWIAANMTPQRGPYTGVI
                     KALRNKLGDCEEMAGIMVALCRASGIPARLVWVPNHNWAEFYLTDHEGEGHWIPAHTS
                     CYAWLGWTGVHELVIQKGDRVTPAHEKRPQRLLEDWLQWIGKQPRARYLAELTPVASP
                     SGGESDAGPGARSKVASGEWKLAGTHPMDRYLRNG"
BASE COUNT          955 a         1835 c         1450 g          945 t
ORIGIN      
        1 gccacgggac atgcggctat cgacgcgccc agggacggca aagacctcgc gatcttgttc
       61 ggctgcgtgg cgagctgaaa tcaacgcccc cgagcggtcg gaggcctcga ccacgatcac
      121 gcccaggctc aatcccgtca cgatgcgatt ccgctgcgga aaggctccgg gcagcggcgg
      181 cgaaagtggc gggttctcgc tgagcacggc gccttgcgcg atgatctcgt cggccaggcg
      241 caagtgctct ggcggataaa cgttgaggac gccgctgccg agcaccgcca gcgttcggcc
      301 cccggctttg agcgcgccgc gatgcgcggc ggcgtcgatg ccgcgggcca ggccgctgac
      361 gatcgtatag ccggcccgcg ccaggccggc cgccagccgt tcggcttgcg tgattccgta
      421 cggtgtgccg tgccgcgaac cgacaatcgc caccgccagc ccatcggtcg gcgcgagcgt
      481 tccacggaca aacaacaccc ccggcggatc gaagatcgac ttcagcggct gcggatagtc
      541 gggatgcgtc tgtgtgacga gctgcacgcc ctgctcgcgg cagcggtcca gctcggcggc
      601 cacgtcgatc tcttggcggg cggcgacaat cgcccggccg tttttcgtgc cgattcccgg
      661 cacatttcgc aaatcgctgg cagacgccgc cagcacagca gccgcactgc caaagtgctc
      721 gacgagcgtc ttccttaggc gggggccaat tcctggaacg agcgacagga gcagctcagc
      781 agcggcatcg tccggaccag attccgattc gggtacgggc gacatgctga gtttcccaaa
      841 gcggcccgct cgctgcccgt ctattgagct gcttgtgccg aagttgctga aagcggggca
      901 gtcgagaata tcattgccgt cggcggcatg ctatcaaccg cgcaaagaaa acggccagag
      961 gcacctgact gtgacaacct gccattgtca cacgagaggg cgtggccgct tgtatcaaca
     1021 cgcatcgaca cgtccggcgt cccacccaaa tacggaggtc cgcaattcaa cattctggaa
     1081 cgccttgcca aagcttcgcc agacagcgcg attggcaaaa ttgacaaatt cgcgggcggc
     1141 aacccgctca ggtgggaagt tccggtttca ggaagccgcc gacgatggcc atgcggacaa
     1201 gctgcggcag cgaatcggcc tgcatcttcc gcatgatgtt ggcgcggcgc atctccaccg
     1261 tccgcaaacc gatatccaga tcggtcgcaa tgcgtttgtt gggcagccct tccagaatct
     1321 tgtgaaaaac ggccaattct tcgctgctga ggttggcaga ccgggcctga acttcagatc
     1381 tctggctgga cccaacctgc cgcacatact cctgttccag cgctttgcga atgccttccc
     1441 acagctcatc aacctggcag ggcttttgga gaaacgtgac ggcccccatt tgcatggctc
     1501 ggacagccat ggggatatcg ccataccccg taatcacgac gacaggaagc ttgctccctt
     1561 gggtggcgag ctgctcgatg agctgcagcc cggtcatgcc gttcatccgc acatccacca
     1621 cgaggcaccc cacctcgctg ggatcgtact ggtccagaaa ctcctcggct gacgcaaatc
     1681 cttgcacccg cagccctttt gactccacca gcgctaccac cgactctcga gccgcctggt
     1741 cgtcatccac cacgaaaaca atgggatcac gatccatagt cgaagctgct tggtccttgg
     1801 cctcgatccc ctcgctcaac ctttcacctc tcgtcccgtt agcgtcttgt tccgctcgcg
     1861 aaatcagccg tttttcgttg catcccaccc cgcataggga ggacacaggc gacattaggg
     1921 cttctttatg gctgatcgcc aagccccccg tccatgcggg ttgccgcaaa ttattgaact
     1981 cggcgtaagg atctgctcca agaacgactc ttggcccaac atgtacctac caagtcgctg
     2041 ccgccaaggc gatctagtcg ctccaggccc ggcgaaccag gtcttcatca accgcactga
     2101 ccagctcgac ctgccccaat cgcgcgggca ggaccaaccg caatcggccg tgctccgcct
     2161 tcttgtcccg gcgcatcgcc gcgagtagtt catcgcggtc caatccttcg atcgcgaccg
     2221 gcaatcccag cttttcgagc agcgcttgct gccggagtcc gagggaaccg tcaattctcc
     2281 ccagcgactc cgccagccgc gcggcgcaca ccattccaat cgacaccgct tctccatgca
     2341 gatattgccg atagcccgtc acggtttcaa ttgcatggca aaaagtgtgg ccatagttca
     2401 gcaccgcccg caagccggtc tcctcccgct cgtcccgctc caccacgctc gctttgagcc
     2461 ggcacgagcg ggcgattacg tgctccagca cttccggctg gcgggcaatc agcttatcgg
     2521 cctgcttctc caaatattca aaaaacgctg cgtccaggat cacaccgtac ttcaccactt
     2581 ccgccaggcc ggagcgatat tcccgctctg gcagcgttga aagcacgtcc agatcgatca
     2641 gcacgcccgc cggctgccag aaggcgccga cgatgttctt cgccgccggc aggttgatcc
     2701 ccaccttccc gccgacgctg ctgtcgacct gcgcgagcag actcgtcgga atttgcacga
     2761 gcgagagccc gcgggcgaac gtggccgcca ccaagcccgc cagatcgccc accacgcctc
     2821 cgcccacggc aaccaccgcc gtcttgcggt ccgcttgccg ccgcaccagc tcattccaca
     2881 gcaactcggc ttgcccaaca gacttgctgg cctcaccggc cggaacggtc acgacatcgc
     2941 acttcacacc tgccgcggcc agcgcccgct caatgggcac tgcgtgccgc ggaccgacat
     3001 tctcgtcggt aatgatcgcc gcctgcttgc acccgggcag ccggccgctg acgaattggc
     3061 cagcaccggc cagcgttccg ctgccgattt cgatatcgta accccgctcg cccaaagcaa
     3121 cgcgaatcgt cgtagccata gcacacacca acccgaagcg tcagcgaggg aatccggatc
     3181 gcccacacga acccgaagcg tcagcgaggg agtgcaggag ttctgccgtc aaccgctcac
     3241 gcgctgcacg tccgcgtcgc tcaccgtgat ctgccggcaa aagcccgtgt gcgtccgcac
     3301 gtactcgagc ccggcgcacg ccgccgggtc ggcgtgcagc tttgcctcca gccgcgcctt
     3361 ctcgacaggc ggcatctctt cataggcctc cggccagatc gtgttggtct ccatcaccag
     3421 ggcttcgcgg tacggatcaa acttgtcagc cacagccagc aatctccaaa aatgcaattc
     3481 tcccctctcc tctcgtgaga gaggggccgg gggtgagggg gcaaaccgac caaccatccc
     3541 ttccaacagc aagtcggcag tatatccacc acgtcccctc tcgtgaatgc agccggccgc
     3601 ttcggagagc tgaccactga caatcgacca ttccgccgtc cttgccgcca aaccaccccg
     3661 ccattacaaa gacccccatg caagcctatc tcgacctcct ccgccacatc ctcgaccgcg
     3721 gcacgcccaa atccgaccgc accggcaccg gcacgaagag cgtctttggc taccagatgc
     3781 gttttgacct ggcggcagga tttccgctgc tcacgacaaa aaaactgcac ctcaagtcga
     3841 ttctgtatga gctgctctgg ttccttcgcg gcgagaccaa catcgcgtgg ctcaaggagc
     3901 gcggcgtcag catctgggac gaatgggccg acgagttcgg cgaactcggc ccggtctacg
     3961 gctaccagtg gcgatcttgg cccgcccccg acggccggaa aatcgaccag atcaccgatg
     4021 tgatccagcg catccaaacc acgcccgatt cccgccgctt gatcgtcagt gcctggaacg
     4081 tggccgatct ggaccgcatg gcccttccgc cgtgtcacct cctatttcag ctgtacgtgg
     4141 ccgagggccg cctctcgtgc cagatgtatc agcgctcggc cgacgtcttc cttggggtgc
     4201 cgttcaacat tgcgtcgtat gcgctgctga cgcggatggt cgcccaggtc acgggcctcg
     4261 cgcccggcga gttcatccac accttcggcg acgcccacct ctacaccaac cactttgagc
     4321 aggcccgcct gcaactcacc cgcgagcccc gcccgctccc cacgctgcgc atcaaccccg
     4381 aagtgcgctc gatcttcgac ttccgctacg aggacttccg tctcgaaaac tacgacccgc
     4441 atccgcacat tcccgctccc gtggccgtgt gaccgcaggt tgcggtgagg cgctcagcga
     4501 tccgggacgc gtgttatcac gcgacttcac gaactttttt ccgcccgcgt tttccggctg
     4561 cttcgctacg gcgggcacga tccactgggc cacggctacc cattccggag ataccggtcc
     4621 atcgggtgcg tcccggcgag cttccattcc ccgctggcca ctttgcttcg cgcgccgggg
     4681 ccggcgtcgc tttctccacc cgacggcgaa gcgaccggcg tcagctccgc gaggtatcgc
     4741 gcccgcggct gcttgccaat ccattgcagc cagtcttcga gcagccgttg tggccgtttc
     4801 tcatgggccg gcgtgacgcg gtcccccttc tggatgacca gctcgtggac ccccgtccag
     4861 ccaagccagg catagcacga cgtatgggcc ggaatccaat gtccctctcc ctcgtggtcg
     4921 gtcagataga attcggccca gttatggttc gggacccaca ccaggcgggc cgggatgcca
     4981 ctagcgcggc acagggcgac catgatgccg gccatttcct cgcaatctcc gagcttgttg
     5041 cgcagcgctt tgatcacacc ggtgtaaggg ccgcgctggg gcgtcatgtt agcggcgatc
     5101 caggcttgga aggattggac gaggttccag gggtgcaaag tagcgggcac actccgggtg
     5161 ccgtcttctg cggattcgtt ggaac
//
LOCUS       DASUGB010001122         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_681013, whole genome shotgun sequence.
ACCESSION   DASUGB010001122 DASUGB010000000
VERSION     DASUGB010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681013"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4028
                     /locus_tag="VFV87_21370"
     CDS             <1..4028
                     /locus_tag="VFV87_21370"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5106390.1"
                     /translation="ALLDQHLPQPLLSVLFGSEDPLSPLHQTLFTQPALFAVEYALCE
                     LWKSWGIRADLALGHSIGEYVAACELGVFGLEDALRLVAARGRLMQQLPLTGAMVAVA
                     ADDRRVRALLESNGHIEVAAVNSPQQTVLSGPEAAIDHAASILEAAGLRTSRLRVSHA
                     FHSSLMEPMLDEFESLCREVPFQQPRAAIASNVHGRLVTDEIASPQYWREQIRSTVRF
                     ADGLEALEQGGIDIYLEIGPRPVLTPAGRQTLTDYGQLWLTSLRPGTDDWLQMLECAA
                     ELYVRGLPLDWEGFYRDHPRRKVVLPNYPFQRSRYWIDSPDRSKSRGDSTSDQSALPA
                     SQESLDDCLFEVQWQPRMRLDQDLPRRSADFIPAADVLRQAVRPEIERLQAQLTLPRY
                     MELSASLERLSARFVLQALAQLGWSPAAGEEFSTAGLAERLGVASRQRLLGRLLDMLA
                     EDGLLARTPIGWQLGTAPAASDPAAEIAALLRRYPECHAELTLLASCAGNLAGVLRGE
                     IDPLQLLFPQGSTAAVEALYEDSPFARSLNALLEETLSRAIADLPVGRPLRILEIGAG
                     TGGTTSHLLPRLPAERTEYVFTDVSQVFLHEARSKFRQFPFLQYAVLDIESDPQAQGF
                     APGQFDLVIASNVLHATRNLRQSLAHVRQLLAPEGALLLLEGTRPERWLDLIFGLTEG
                     WWRFADDEFRPKHPLATAETWRHVLAQQGFADFAALPQTGVGQTEAPQAVIFARLGTV
                     SPPAAETSPEPESSRDETPVSKWLILADRHGVGSALAAALEAAGHECALLPADRSRDL
                     CGLLESHNFAGIVHLWGLDTASADELSVDRLQEAQWLACESAIEVCQELVSQASEAPP
                     RLWLVTRGAQAVATGDQLPGLAQSPLWGLGRAVAEEQPTLFGGLVDLDPVLEPHEAAL
                     LLAAELQKPDGENQVAYREHGRYAARLVRRPPESGSHQPLRWRTDGSYLVTGGLGEIG
                     LQVAAWMVDQGAHHLILTDRTARESERVRRLEERGVTIRVAAVDVTDEAAMRDLLHSL
                     AAEGWPPIRGVVHAADVADAQPLAAIRSERLMEVVGSKLIGGWLLHKLLRGERLDFFV
                     CCALADALLPTPEHVLPAAAGEFPGALAHYRRAQGLPALTVNFATPEGLSALEHLLER
                     GATQTAITPLDLTASQSSLLANLRLMELEPEAPMAAQGSSGVSPRAAMVSAAPQKRRE
                     LVESYLRDQISRVLRADPARLDAHQSLGSLGIDSLMAVELRNQVQAHLEVTIPLARLM
                     QDPTIYQLAQTVLDQWGVAPPPTSAAASSPADRPVDSPDPPAKPRQVPAPATLDQVQD
                     LSDEEVDALLRQMLNEEQTQA"
BASE COUNT          629 a         1392 c         1391 g          711 t
ORIGIN      
        1 ccgcgttgct cgatcagcac ctgccgcagc cgcttttgtc ggtcctgttt ggcagcgagg
       61 acccgctctc gccgctgcac cagacgctgt tcacgcagcc ggccctcttc gcggtggaat
      121 atgcgctctg cgaattgtgg aagtcgtggg gcatcagggc ggatttggcc ctcgggcaca
      181 gcattggcga gtacgttgcc gcctgcgaac tgggcgtctt tggcttggaa gacgcgctcc
      241 gactggttgc ggctcgcggg cgattgatgc aacagttgcc gctgaccggg gccatggtcg
      301 cggtggcggc cgacgaccgg cgcgtgcggg ccttgctgga atcgaacgga catatcgaag
      361 tggctgcggt caatagtccg cagcagacgg tgctctccgg cccggaggcg gccatcgatc
      421 atgctgcatc gatcctggaa gcggccggcc tgcggaccag ccgcctgcgc gtttcccacg
      481 cattccattc gtcgctgatg gagccgatgc tggacgaatt tgagagtctg tgtcgcgagg
      541 ttccattcca gcagccgcgg gccgcgatcg cttcgaacgt ccacgggcgg ctggtgacag
      601 acgagatcgc ttcgccgcag tattggcggg agcagattcg cagcacggtc cgctttgccg
      661 atggtttgga agcgctggag cagggcggca tcgacatcta cctggagatc ggcccgcggc
      721 ctgtgctgac gccagccggc cgccaaacgt tgaccgatta cgggcaactg tggctcacga
      781 gcctgcgccc cggcacggac gattggctgc agatgctcga gtgtgccgcg gaattgtacg
      841 tgcgcggcct gccgctcgat tgggaaggct tctaccgcga ccacccgcgg cgaaaggttg
      901 tcctgccgaa ttatccgttc cagcggtcgc gatattggat cgattcgccg gaccgatcga
      961 agtcgcgcgg agattcgacc tccgatcaat ccgctctgcc agcctcccag gagtcgctcg
     1021 acgactgtct gttcgaggtc cagtggcagc cgcggatgcg tctcgatcag gacttgccgc
     1081 gccgcagtgc ggatttcatt ccggccgcgg atgtgctccg ccaggccgtg cggccggaaa
     1141 tcgagcggct gcaggcgcag ctcacgctgc cgcggtatat ggagctgtct gccagtctgg
     1201 agcggttgag cgcccggttc gtgctgcagg ctctcgcgca gctcggctgg tcgccggcgg
     1261 ctggcgagga gttttccacg gcggggttgg ctgagcgatt gggcgtcgcc agccgccagc
     1321 ggctgctggg ccgcttgctc gacatgctgg ccgaggacgg gctgctcgcg cggactccaa
     1381 ttggctggca actcggaacg gcgccggccg cgagcgatcc cgccgcggag attgccgcgc
     1441 ttttgaggcg ctatcccgag tgtcacgccg aactaacgct gctggcttcc tgcgcgggca
     1501 acctggccgg tgtcctgcgc ggcgaaatcg accccctgca gctcctgttc ccgcagggct
     1561 cgaccgccgc ggtcgaagcg ctctacgagg attcgccgtt tgcccgctcg ctcaacgcgc
     1621 tgctcgaaga aacgctctcc cgggcgattg ccgatctccc cgttggacgt ccgctccgga
     1681 ttctcgaaat cggcgccggc accggcggaa ccacttcgca cctcctgccg cggctgccgg
     1741 cagagcgcac cgaatatgtc ttcacagatg tgtcgcaagt cttcctgcac gaggcgcggt
     1801 cgaagtttcg gcagttcccg ttcctgcagt acgcggtgct cgatatcgaa tccgatccgc
     1861 aggcgcaggg ctttgcaccg ggccagttcg atctggtgat cgcttccaac gtgctgcacg
     1921 ccacgcggaa cctgcgccag tcgctcgctc acgtccgcca gctcctcgcg ccggaaggag
     1981 cgctgctgct tctggaaggt acgcgccccg agcgttggct ggacctgatc tttggcctga
     2041 ccgaaggctg gtggcggttt gcggacgacg agtttcgtcc aaagcacccg ctggccactg
     2101 ccgagacgtg gcgtcacgtg ctcgcccagc agggttttgc ggatttcgcc gccctcccgc
     2161 agaccggagt cggccagacc gaggccccgc aggcggtgat ctttgctcgc ctggggacgg
     2221 tctcgccgcc ggcggctgag acctcgcccg agccagaaag cagccgcgat gagaccccgg
     2281 tcagcaagtg gctgattctc gccgaccgcc acggcgtcgg gtccgctttg gccgcggcgc
     2341 tcgaggcagc cggacacgag tgcgccctgc tgccggcgga ccgcagccgc gatttgtgcg
     2401 gcctcttgga aagccacaac ttcgccggca tcgtccactt gtgggggctc gacacggcca
     2461 gcgcagacga actgagcgtc gatcgcctgc aggaagcgca gtggctggcg tgcgaaagcg
     2521 ccatcgaggt ctgccaggag ctggtttcgc aggcgagcga agcgccgccg cgactgtggc
     2581 tggtcacgcg cggggcgcag gccgtcgcca cgggcgatca gttgccgggc ctggcccaat
     2641 cgcccctctg gggcctggga cgcgccgtag cggaagagca gccaacgctc tttggcggac
     2701 tggtcgatct cgatccggtt ctggagccgc acgaggccgc gctgctgctc gcagcggagc
     2761 tgcagaaacc cgacggcgag aaccaggtcg cctatcgcga gcacgggcgc tacgccgccc
     2821 ggctggtgcg ccgcccgccg gaaagcgggt cgcaccagcc tctgcgctgg cggaccgacg
     2881 gcagctattt ggtgaccggc ggcctaggcg agatcggctt gcaggtcgcc gcgtggatgg
     2941 tcgaccaagg ggcgcatcac ctgatcctca cggaccgcac cgcgcgggag tccgagagag
     3001 ttcgccggct ggaagagcgc ggcgtgacga ttcgcgtggc cgcagtagat gtcacggacg
     3061 aagcggccat gcgggacttg ctccacagcc tggccgcgga aggctggccg ccgattcgcg
     3121 gcgtcgttca cgcggccgac gtggccgacg cgcagcccct cgctgccatt cgctccgagc
     3181 gactgatgga agtcgtcggc tcgaagctga tcggaggctg gctcctgcac aaattgctgc
     3241 gcggcgagcg gctggacttt ttcgtgtgct gcgcattggc cgacgccctg ctgcccacgc
     3301 ccgagcatgt cctgcccgca gccgccggcg agttcccggg cgcgctcgcc cactatcgcc
     3361 gcgcccaggg tttgccggct ttgacggtga acttcgcgac gccggaaggc ttgtctgccc
     3421 tggaacacct cttggagcgc ggagccactc aaacagccat cacgccgctc gatctgaccg
     3481 cgagccagtc gtcgctgctc gccaatctgc ggctgatgga gcttgagccc gaggcgccaa
     3541 tggctgcgca gggcagcagc ggcgtctcgc cgcgagccgc catggtgtcc gccgcgccgc
     3601 agaagcgccg cgaactggtc gagagctacc ttcgcgacca gatcagccgc gtgctgcggg
     3661 ccgatcccgc caggctggac gcgcatcaat cgctgggcag tctgggaatc gattcgctga
     3721 tggccgtgga gctgcgaaac caggtccagg cgcatctgga ggtcacgatt ccgctggccc
     3781 gcctgatgca ggacccgacg atctaccagc ttgcccagac cgttctcgat cagtggggtg
     3841 tagcgccccc gcccacatcg gccgccgcat cttcgcccgc cgatcgcccc gtcgattcgc
     3901 cggatccgcc ggccaaaccc cgtcaggtcc cggccccggc cacgctcgac caggttcagg
     3961 acctttccga cgaagaggtt gacgcccttc ttcgccagat gctcaacgag gagcaaaccc
     4021 aagcatgagt cagcttgccc aagaattgtc gtccgacgaa aagcgccgcc tgctctcgca
     4081 gcttttggag cgcaaggccc gcgaagaccg cgagaacgcg gcc
//
LOCUS       DASUGB010001123         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1655338, whole genome shotgun sequence.
ACCESSION   DASUGB010001123 DASUGB010000000
VERSION     DASUGB010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655338"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2422)
                     /locus_tag="VFV87_21375"
     CDS             complement(<1..2422)
                     /locus_tag="VFV87_21375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106391.1"
                     /translation="MPAVWNPQANAIFLKALEQPPTEREPFLQQACGQDASLAEQVRA
                     LLASSDQAGCFLESPPTAVAKLAETAKGRAAGPPRLSFLAPSEIPDSLGRLGHYIILG
                     VVGSGGFGVVLKARDEKLQRIVAVKTLAETLAASGAARQRFVREAIAAAAVRHENVVA
                     IHAVDDEGPVPFLVMEYVEGQSLQSRLDKTGSLTIPEILRIGLQIAEGLAAAHQQGLI
                     HRDVKPANILLENGVPRVKITDFGLARLADDAAQTVTGLIAGTPAYMSPEQARGEAID
                     HRSDLFSLGSVLYAMCTGQSPFRGSTTLGVIKRVCEEQPRPIRDLNSDIPEWLCGIIE
                     RLHAKCPDERYRKAAEAAEDLRRGLSHVQGGASQPASQEPEFAIRPHRAPEAPGRTIR
                     RWFAPAVAVALLLTLMFTLAEAGGVTQLVPTVIRIVTGEGTLLVETSDPDVKVTIEGD
                     GGLVITGAGPQDLRIRPGTYRIKAAKDGRPLPLDSELVTVTRGGVEVVRVTLEAKSTA
                     GTIENGSIPAVPEAAATEATLREAIRLMPRDSENHKRLGEYLDQHNRLPDAEAAYREA
                     IRLVPSESHYYVLLGGVLLKQPDRYTDGIAELERARNLSPNYAYINSRLGDAYANLGR
                     WREAAAPIRRNSDLTPSEHWGNFKHATLSLYLGDLENYRIACQRLLDAHDAGKDDDPD
                     NTATVCLLSPGGIGQDERVRQIALDGRLQQGNARKQRNARALAELRAGHWHSAIDEVG
                     TFNRDLLDPFGAQLRLISLAILAMAQKGSGDRPAAMQTLANAKALRAATWPNPEQDRY
                     FKESWSGWL"
BASE COUNT          410 a          738 c          795 g          499 t
ORIGIN      
        1 gaagccaacc gctccaagac tctttgaaat agcggtcttg ctcgggattg ggccacgtgg
       61 ctgcccggag cgccttggcg ttggccaagg tttgcattgc tgctggccgg tctcctgagc
      121 ccttttgagc catcgccaag atggccagcg aaatcaaacg gagctgcgct ccaaaaggat
      181 cgaggagatc gcgattgaag gttccaacct cgtcgatggc ggagtgccag tgtccggccc
      241 tcagttctgc cagggctcgc gcattgcgct gtttccgagc gttcccctgc tgaagtcgcc
      301 cgtcgagtgc gatctggcgc actcgctcgt cctggccaat tccgccgggg gagaggaggc
      361 acacggtagc cgtgttgtct gggtcgtcgt ctttgcccgc atcatgggcg tccagcagcc
      421 gctggcaggc gatacggtaa ttttccaagt cgcctagata aagcgacaag gtcgcatgtt
      481 tgaaattccc ccagtgttcg gaaggcgtca gatccgaatt gcggcggatt ggcgcggcag
      541 cttctcgcca tcgtcccaag ttggcgtagg catctccgag acgggaattg atgtaggcgt
      601 agtttgggct caggttacgg gccctttcga gttccgcgat gccatcggtg tagcggtccg
      661 gttgcttcag cagcactcca ccgagcagga cgtaatagtg actttcgctt ggcacgaggc
      721 gaatcgcctc gcgataggcc gcttcggcgt caggtagccg gttgtgctgg tccagatatt
      781 cgcccaggcg cttgtggttt tcagagtcgc ggggcattag ccgaatcgcc tcccgtaaag
      841 tcgcctcggt agcagcggct tctgggactg cggggatgct gccgttctct atagtgcccg
      901 cagtcgattt ggcttcgagc gtgactctaa caacttcaac tccgccgcga gtgacggtca
      961 ccagttctga atcgagcggg agcgggcgac cgtccttggc agctttgatt cggtaagtgc
     1021 ctgggcggat gcgaagatcc tgcggacccg cgcccgtgat caccaggccg ccgtcccctt
     1081 cgatcgtgac tttcacgtcg gggtcgctgg tctcgaccag cagagtcccc tcgccggtga
     1141 cgatgcggat gaccgtcggc accagttgcg tcacgccacc cgcttcggcc agggtgaaca
     1201 taagcgtcag taagagcgcc accgcgacag cgggtgcgaa ccagcggcga atggtgcgcc
     1261 cgggcgcctc tggtgcgcga tgcggacgga tcgcgaattc aggttcctgg ctggcgggct
     1321 gggaggctcc gccttgaacg tgcgacaggc cgcgccgcaa atcctctgcc gcctccgctg
     1381 ctttccgata gcgctcgtct gggcacttgg cgtgcaagcg ctcaatgatg ccgcacagcc
     1441 actcggggat atcgctgttc aggtcgcgga ttggccgcgg ctgctcctcg cagacgcgtt
     1501 tgatcactcc cagtgtggta ctgccgcgga acggcgattg gccggtacac atggcataga
     1561 ggacactccc caggctaaag aggtcagagc ggtgatcgat cgcttcgccc ctggcctgtt
     1621 cggggctcat gtaggcgggt gtgccggcga tcagacccgt gacggtctgc gcggcgtcgt
     1681 cggctaagcg ggcgagcccg aagtcggtga tcttgacgcg cgggacgccg ttctccagca
     1741 ggatattggc cggcttcacg tcgcggtgta tgagtccttg ctgatgtgcg gccgccagtc
     1801 cttcagcaat ttgcagtcca atccgcagaa tctcagggat tgtcagagag cccgttttgt
     1861 cgagcctgga ttgcagcgac tggccttcga catattccat taccaaaaac ggaaccggcc
     1921 cttcatcgtc gaccgcgtgg atcgcgacga cgttctcgtg gcggacggca gctgcggcaa
     1981 ttgcttcgcg cacaaaccgc tgccgcgcgg cgcccgaggc cgccagtgtc tcagccaagg
     2041 ttttgacggc gacgatccgc tggagcttct cgtcgcgggc cttgaggacc acgccgaatc
     2101 ccccgctgcc gacgacgcca aggatgatgt aatgccctaa acggccgagc gaatcgggta
     2161 tctctgacgg tgcaaggaag ctgagtcttg gcggtcctgc tgccctgccc tttgcagttt
     2221 cagcgagttt ggctacggca gtgggcggcg actccaagaa gcaacccgcc tggtcgctgg
     2281 aggccaagag ggcgcggacc tgctcggcga gcgaggcgtc ctggccgcaa gcctgctgca
     2341 gaaatggctc gcgctctgtg ggcggctgct ccagcgcttt caggaagatc gcgttggctt
     2401 gtggattcca aacggcgggc acgggacacc tccggctgct at
//
LOCUS       DASUGB010001124         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1793809, whole genome shotgun sequence.
ACCESSION   DASUGB010001124 DASUGB010000000
VERSION     DASUGB010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793809"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFV87_21380"
     CDS             <1..588
                     /locus_tag="VFV87_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106392.1"
                     /translation="RTVRVSTDIDHRIDELLPGGLADYSICLKMATNSPALGMIDELS
                     QSASDPRLLETDRQVQSLCEIYNDANDSERIRIRQRFRGKMSFSHFASRMAIKVLRGG
                     PCNYITDGLVALCIEDFAFDPRDSLMSLAALQHAARTTGLDFPIAVAAAMDLSSESVK
                     SQLSWFVKRGPENTKLGDFALREYHDSNGAMIGFA"
     gene            complement(835..1233)
                     /locus_tag="VFV87_21385"
     CDS             complement(835..1233)
                     /locus_tag="VFV87_21385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5106393.1"
                     /translation="MATCYFAATGAEVTTVDLPESAFNLPSVDDTLAACGLAAEVVRK
                     PSREAMAEWAAVGRQFDLVYVDGSHTLGDALTDLLLADLLLVPGGWLLVDDIDNADFP
                     GVRTAWEHFLRLRGYDRDDRPNWGVGRRPT"
     gene            complement(1163..2134)
                     /locus_tag="VFV87_21390"
     CDS             complement(1163..2134)
                     /locus_tag="VFV87_21390"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5106394.1"
                     /translation="MNKPTLPLPRGWTNAVRAAVLHVIALAQFSLACAEGQLTRRKGH
                     RGRLRAEHDRLIQELELVRQELRIKDARLSRLAPQKRPHYLPSERMAILQLRAARGWT
                     VDETAAAFQVTPLTIREWMGRLETEGTERFLSLGTPVNKFPDFVREVVQRLKSHCPRL
                     GKRKIAETLARAGLHLGATTVRRMLREFPKPKPQAASLRCRGGRLHSHVHQRQHVGRA
                     ALREAAVRLAGADRSRAAAPVRRPVVERALGPRLHSPVPHDRGRAGRAKGAEVAELGQ
                     RPVRGQPGCQARPEADPGDRHAARRGHVLLRGDRGGGHDGRSAGVGV"
     gene            complement(2148..2333)
                     /locus_tag="VFV87_21395"
     CDS             complement(2148..2333)
                     /locus_tag="VFV87_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106395.1"
                     /translation="MSYLRAVVQLVYQGAWRAILLWRLRRQGIDPDTIPEIQELRRRR
                     DAGEPLEPDEPPQSHPP"
     gene            complement(2342..>2633)
                     /locus_tag="VFV87_21400"
     CDS             complement(2342..>2633)
                     /locus_tag="VFV87_21400"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX5106396.1"
                     /translation="NAFVERFIQSISQECLDRFVIFGEKHFNHLCAEYLAHYHEERPH
                     QSLDNRPLKQTIPRGRPTNHFGPLVEQVVPLSEVRCQERLGGLIKHYYRKAA"
BASE COUNT          471 a          838 c          813 g          511 t
ORIGIN      
        1 aggacagtaa gagtgagcac cgatattgac catcgcattg atgagctact cccaggcggc
       61 ttggcagatt atagcatttg cttaaaaatg gctacgaatt ctcctgcgct aggaatgatt
      121 gacgagttgt cccaaagcgc aagcgatcct cgcttactgg aaacagaccg ccaggttcag
      181 tcactttgtg agatttacaa cgacgccaac gactccgaac gaattcggat tcgacagcga
      241 ttcagaggta aaatgtcctt cagtcatttc gcctctcgaa tggcgattaa agtcttgcgc
      301 ggcggacctt gtaactatat cacggacgga ctcgtcgcgc tgtgcattga ggatttcgcc
      361 tttgatcctc gcgactcgct aatgagccta gcagcgctgc aacatgcggc gcgtacaacg
      421 ggcctagact ttccaattgc agtagcggca gcgatggacc tatcttctga aagcgtcaag
      481 agccagttga gttggtttgt taaacgtggt ccagagaata ccaagcttgg cgacttcgcg
      541 ctccgcgaat accatgactc gaatggggcg atgattgggt ttgcctagtt ggacagcgcc
      601 acgttcggtt ttctgatctc ggttaagtcg tggctgagca ggagtttatt gctgttgacg
      661 ggaagggaag aaagagacga ccctccgtgt gatttctgtt tcctagggaa accacggagg
      721 gcgggaaaag gggtcaggct tgggttaggc ttcaatattg ggcgacaata gtgcgaatca
      781 aagggacatt cttgatctgt cgagtactcc acgctagcgc cgggtgcccg gtttctaggt
      841 gggccgcctc cccacgcccc agttcggccg gtcgtcgcga tcgtaccccc gcagccgtag
      901 gaagtgctcc caggccgtgc gcacgccggg gaagtccgcg ttgtcgatgt cgtccacgag
      961 cagccagccg ccgggcacca gcagcaggtc ggccagcagc aggtcggtga gcgcgtcgcc
     1021 cagcgtgtgt gacccatcga cgtacaccag atcgaactgc cgcccgaccg ccgcccactc
     1081 cgccatcgct tcgcggctcg gcttgcggac gacttccgcg gccaggccgc acgccgccaa
     1141 cgtgtcgtcc acgctcggca ggttaaacgc cgactccggc agatcgaccg tcgtgacctc
     1201 cgccccggtc gccgcgaagt agcacgtggc cacgccgtgc agcgtgccga tctccaggat
     1261 ccgcttcagg ccgtgcctgg caaccaggtt ggccacgaac cgggcgttgc cccagttcag
     1321 ccacttcagc tcctttagca cggcccgcac gtcctcgatc gtggggcact ggcgaatgca
     1381 gccgtggccc cagtgctcgc tccaccactg gacggcgtac aggtgccgcc gctcgcgatc
     1441 ggtcagctcc cgcgagccgg acagcagctt ctcgaagtgc tgctcgcccg acgtgctgcc
     1501 gctggtgaac gtgcgaatgt aatcggccgc cccggcatcg cagactcgca gcttgcggct
     1561 tcggcttggg aaactcccgc agcatccgcc gcaccgtcgt cgcgcccagg tgcagcccgg
     1621 cccgcgccag cgtttcggcg atcttccgct tcccgagccg ggggcagtga gacttcagcc
     1681 gctgcaccac ctcgcggacg aagtcgggga acttgttgac gggcgtgccc agactgagaa
     1741 accgctcggt cccctccgtc tccagtcggc ccatccactc gcggatcgtc agcggcgtca
     1801 cctgaaacgc ggccgccgtt tcgtccaccg tccagccgcg ggccgcccgc aactgcagaa
     1861 tcgccatccg ctcgctcggc aggtaatgcg gccgtttctg cggcgcaagg cggctcaggc
     1921 gggcgtcttt gatccgcagc tcctggcgga cgagctccag ttcctgaatc agccgatcgt
     1981 gctcggcccg cagccgaccg cgatgccctt tgcggcgtgt cagttggcct tcggcgcagg
     2041 cgagcgagaa ctgggccagc gcgatgacgt gcagcacggc cgcccgtacg gcattagtcc
     2101 agccgcgcgg caacgggagc gtgggcttgt tcatcggcgg tgtcgcgtca cggtggatga
     2161 ctctgaggtg gctcgtctgg ttcgagtggc tcgcccgcat cgcggcggcg gcgcagttcc
     2221 tggatttcag gaatcgtgtc gggatcgatt ccctgccggc gcagccgcca gagcaggatg
     2281 gctcgccagg cgccttgata gacgagctgc acgacggccc gcaggtagct catcgcagga
     2341 cctacgctgc ctttcgatag tagtgcttga tcagcccgcc cagccgctct tggcagcgca
     2401 cctccgacag cggcacgacc tgctctacca gcggcccgaa gtggtttgtc ggccggccac
     2461 gcggaatcgt ctgcttcagc ggcctgttgt ccagtgactg gtgcggccgc tcctcatggt
     2521 aatgggctag atactcggcg cagagatgat tgaagtgctt ctctccgaag atcacgaacc
     2581 ggtcgaggca ttcctgcgag atcgattgga tgaaccgctc gacgaacgcg ttc
//
LOCUS       DASUGB010001125         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_820658, whole genome shotgun sequence.
ACCESSION   DASUGB010001125 DASUGB010000000
VERSION     DASUGB010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820658"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFV87_21405"
     CDS             <1..341
                     /locus_tag="VFV87_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX5106397.1"
                     /translation="HSKSSAAPELWAQLAMQHDGQDRWYLEALGLAADKQWDAFLDAY
                     LALAGDSARSTPAGRDIIWRSRAKKTPELLVKILKDPATKEEDQPRYFRAFDFLNGPE
                     KEAALKSLLE"
     gene            504..1589
                     /locus_tag="VFV87_21410"
     CDS             504..1589
                     /locus_tag="VFV87_21410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5106398.1"
                     /translation="MFGGASAEEVSAAQRAKDALIVKTLLRLPGVDLSAKPEAKAALL
                     RHLETLAGTDQYLELVEKFNLREAKDELLKLAVEQGESTAGVKAAGLLVKFNEREMLT
                     KAIGDPDPAKGAKLVTAIGLLSDAKTNELLTPLIADSEQPLAIRAGAVGAVGRNAPGQ
                     KWLLELVASGKLADDLKFTAANVLLSSADDEIRNAAGKHLSLPATAGGEPLPPIAELV
                     KRSGDAARGKELFATTGTCAKCHKVKGEGKEVGPDLSEIGSKLSPEAMYLSILDPSAG
                     VSFNYETWALRTFDGTVLTGILVSQTDDAVELKTAEAIVHKLSRDDVEQMKKSSLSIM
                     PADIPKLLKAQDLVDIVQYLTTLKKAP"
     gene            1724..2788
                     /locus_tag="VFV87_21415"
     CDS             1724..2788
                     /locus_tag="VFV87_21415"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX5106399.1"
                     /translation="MADEKADRLDRTPRQVAEQLAQVYGHKLDQVAYIPALPLVAKLR
                     LSELTGDASHADEVAKIVAPYLSGEKSPVPKSGSEQAGHLIFAELAARGGRPLTPNPS
                     RTRGEGNRARERWISLCRAAADQIFGNDGQPLELMPFHNEMSDAVFMAGPIMAATGKL
                     TGEAKYFDAAATHFASMRKLCLREDGVYRHSPLCEAAWGRGNGFPALGLAWALSEWPD
                     EHPAKKELIGELQKHLAALKKHQDQTTGCWHQVIDHPESYDEYSCTCMIGWAMQRGIS
                     RGWLAKDEYQPCVDLAWRAVKERTSPTGELVNVCTGTGKQKTLQDYFDRPAINGRDDR
                     GGAMGMMFATELLAAERKGQ"
     gene            2831..>3418
                     /locus_tag="VFV87_21420"
     CDS             2831..>3418
                     /locus_tag="VFV87_21420"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106400.1"
                     /translation="MLRALMLVACGVGAAAAHAEDWPHWRGPSRDGHSAEASGFNDGK
                     WLDEKPVWTINVGAGGSSPIVVGETVYFLGWSGGKDHLQAVDLKSGKKLWTSSYESPQ
                     YARHATGDEGLYRGPSSTPEFDLDTGIVYTLSLDGELRAHDSKGSEKLVWRRNLYDEY
                     QMPQRPRIGRSGRRDYGYTTAPLVHGEWLIVEAGGN"
BASE COUNT          666 a         1030 c         1151 g          571 t
ORIGIN      
        1 gccacagcaa gtcgtccgcc gcgccggaac tgtgggcgca gctcgccatg cagcacgacg
       61 gccaggatcg ctggtatctc gaagccctcg gcctcgccgc cgacaagcag tgggatgctt
      121 ttctggacgc ttacctggct ctcgccggcg acagcgcccg ttccacgccc gctggccgcg
      181 acatcatctg gcgcagccgg gccaagaaaa caccggagct gctcgtcaaa atcctcaaag
      241 accccgccac gaaggaagaa gatcagcccc gctacttccg ggccttcgac ttcctcaatg
      301 gccccgagaa ggaagcggcg ctgaagagct tgctggagta aggggctact ggcgcagcgg
      361 attcttttga atagaagctc gcaaagatcg caaagaagag ggattcggtg actaagtttg
      421 tgcggcactc ggtgcgtttc tgttttgctt ctttgcgtcc tttgcggcct tctgttccaa
      481 tttccgccct gctgttgttc gcaatgtttg gcggcgccag cgcggaagaa gtgtcagccg
      541 cccagcgggc gaaggatgcg ctaatcgtca agacgctgct gcggctgccg ggggtggatt
      601 tgagcgcgaa gccggaggcg aaggcggctc ttttgcggca tttggagacg ctggcgggga
      661 cggaccagta tctggagctg gtcgagaagt tcaacctgcg cgaggcgaag gatgagctgc
      721 tgaagctggc ggtcgagcag ggggaatcga cggcgggcgt gaaggcggcg gggctgctgg
      781 tcaagttcaa cgagcgggag atgctgacca aggcgattgg cgatcccgat ccggccaagg
      841 gggcgaagct ggtgaccgcg atcggcctgc tctccgatgc gaaaacgaac gagttgctca
      901 cgccgctgat tgccgacagt gagcagccgc tggcgatccg cgccggcgcg gtcggcgcgg
      961 tcggccgcaa tgctccgggg cagaaatggc tgctggagct ggttgccagc ggcaagctgg
     1021 ccgacgacct gaagtttacc gcggcgaatg tgctgctgtc gtcagccgat gacgaaatcc
     1081 gcaacgcagc cggaaaacat ctgtcgctcc ctgccaccgc gggcggcgag ccgctgccgc
     1141 cgattgcgga gctggtgaag cgctcgggcg acgccgcccg cgggaaggag ctatttgcga
     1201 ccaccggcac ctgcgccaag tgccacaagg tgaagggcga aggcaaggag gtcggcccgg
     1261 acctttcgga aatcggcagc aagctgagcc cggaggcgat gtacctctcg attctcgatc
     1321 cgagcgccgg cgtaagcttc aactacgaaa cctgggccct ccgcacattc gacggcacgg
     1381 tgctcaccgg cattctcgtc agccagaccg acgacgcagt cgagctgaaa acagccgagg
     1441 cgatcgtcca caaactctcg cgcgacgacg tcgaacagat gaagaagtcg agcctctcca
     1501 tcatgcccgc cgacatcccg aaattgctta aggcgcagga cctggtggat atcgttcagt
     1561 acctgacgac actgaagaaa gctccctaac gagcctaacg attatgcaaa ttctcacgca
     1621 gaggcgcgga ggcgcagagt ctaattcact cctttccctc tgcgactccg cgcctctgcg
     1681 tgagatttcc tgtttgatcg ttcttgtcgc aggttcgaat gtgttggcag acgaaaaggc
     1741 cgatcgcctc gaccggacgc cgcggcaggt ggccgagcag ctcgcgcagg tctatggcca
     1801 caagctcgat caggtggcct atatccccgc gctgccgctg gtggcgaagt tgcggctgag
     1861 cgagctgacg ggtgatgctt cgcacgcgga cgaggttgcg aagattgtcg cgccgtactt
     1921 aagcggcgag aaatcgccgg ttcccaaatc cggcagcgag caggcggggc atttgatctt
     1981 cgcggagctg gcggctcgtg ggggtcgccc cctcacccct aacccctctc gcacgagggg
     2041 agaggggaac agagcgcgcg agcggtggat cagcctgtgc agagctgccg ccgatcagat
     2101 ctttggcaac gatggccagc cgcttgagct tatgccgttt cacaacgaaa tgagcgacgc
     2161 agtgtttatg gccgggccaa ttatggcggc gacgggcaag ctgacgggcg aggcgaaata
     2221 ctttgacgcg gcggccacgc atttcgcctc gatgcgcaag ctctgcctgc gcgaggacgg
     2281 cgtctatcgc cattcgccac tgtgcgaggc ggcctggggg cgcggtaacg gatttccggc
     2341 gctcgggctg gcgtgggcct tgtcggaatg gccggacgag catccggcca agaaagagct
     2401 gatcggcgaa ctgcagaagc atctggccgc gctgaaaaag caccaggacc aaacgaccgg
     2461 ctgctggcat caggtgatcg accatccgga gagctacgac gaatactcct gcacgtgcat
     2521 gatcggctgg gcgatgcagc ggggcatcag ccgcggctgg ctggcgaagg acgagtacca
     2581 gccctgcgtc gatctggcct ggcgggccgt caaagagcgg accagcccga ccggcgaact
     2641 ggtcaacgtc tgcaccggca ccggcaagca gaagacgctg caggattatt tcgaccgccc
     2701 ggcgatcaac ggccgcgacg accgcggcgg ggcgatgggg atgatgtttg cgaccgagtt
     2761 gctggccgcg gagagaaagg gccagtaaca gttcggctga agggcgccac cagcaaccgg
     2821 aggactaatc atgctgagag cgctcatgct cgtcgcctgc ggagtcggtg ccgctgccgc
     2881 tcatgccgag gattggcccc actggcgcgg tccgtcgcgc gatggccact cggccgaagc
     2941 atccggattc aacgacggca agtggttgga cgagaagccg gtttggacca tcaacgtcgg
     3001 agcaggcggt tcgtcgccga tcgtcgttgg cgagaccgtc tatttcctcg gctggtcggg
     3061 cggcaaggac catctgcagg cggtcgatct gaagagcggc aagaagctgt ggacatcgtc
     3121 gtacgaatcg ccgcagtatg cccgccacgc gacgggcgac gaaggactct atcgcgggcc
     3181 cagctccacg ccggagttcg acctcgacac cggtatcgtt tacaccctca gccttgacgg
     3241 cgagctgcgg gcgcacgact cgaagggcag cgagaagctc gtctggcggc ggaacctgta
     3301 cgacgagtat cagatgcccc agcggccgcg gatcggccgc tccggccggc gggattacgg
     3361 ctacacaacc gccccgctgg tgcacggcga gtggctgatc gtcgaagccg gcggcaac
//
LOCUS       DASUGB010001126         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1656168, whole genome shotgun sequence.
ACCESSION   DASUGB010001126 DASUGB010000000
VERSION     DASUGB010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656168"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFV87_21425"
     CDS             complement(<1..623)
                     /locus_tag="VFV87_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106401.1"
                     /translation="MNYTTSFAYDTDTLRLETITHPNSTTWQLTPQQTMGWASGTGNS
                     LYDPADAVAQYIDERSNTWKFQTDRFGNVIHFQDPLSNVTTTERDHDGRPIRITQADP
                     DGGGALTSPITKLGYSAAGDQVKAYLPDGNSQTWTYHSTWHLPLTYTNELGKTWENTY
                     ELSTGNLLTSEDPLSNVTSYTYTLIGLLESVTTPDPDGGGGLSASVT"
     gene            complement(1022..1804)
                     /locus_tag="VFV87_21430"
     CDS             complement(1022..1804)
                     /locus_tag="VFV87_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106402.1"
                     /translation="MAPSLAEDRIAGNHVRMTGLRFGPYRMPRCKIGGKLDCEYAGTL
                     RVVAISDAPVQWPLGVVWGHRIPVVTGDLVRAVKQEANCDVAVAWGVGASLVSRWRKA
                     LRVKRTKGTRLRRSESIRATDPARARKIAKAKRGVARPPHVIETMRKAHLGKPLPEAT
                     RKKMSATHRKRGTRPPGQNPPWSSKEDRLVRTKRPAEVVRLTGRTWSAVVSRRYVLGI
                     SRRWTPKLDQIVRTKPPAEAAKLTGRTLLAVYKRRWFLGNQE"
     gene            1803..2279
                     /locus_tag="VFV87_21435"
     CDS             1803..2279
                     /locus_tag="VFV87_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106403.1"
                     /translation="MPKVVFQAVLTETGTPPKESPTESIEGAITTALEKQLPEELREI
                     FELDSKVKVNKIHRGSYWIEFTVTFGTIFAIYVGISRYKNFVESVKLIAAHAQAVLSA
                     ATKAYGKFSVSVRPIQGMWPLARRLFYAEFWWVVLLIELSIIALLTIALLVKQLLA"
     gene            complement(2352..>4280)
                     /locus_tag="VFV87_21440"
     CDS             complement(2352..>4280)
                     /locus_tag="VFV87_21440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5106404.1"
                     /translation="LVDDTLVARTSLTTLDNVVCKPGRIYEIPAGSTVNITGDALVPA
                     GSRLVIGNGARLNWQGRGVLEIEGVVTAGSLAKDAAPVVFDFGADPAQEGGLLIHGSL
                     VAGSTFENCQFLHGHGTRLTRANPRTADDIDWNRVAKTVTRSPDARHPVGGAVCLVSA
                     PLVSFRNCEFKRNAANQGGALYCINAKRLVVERCKFLENISGFSGGAIFAHGSELYAS
                     DCRFANTRTGIVWSGLAPDGAPTVGNEFASGGAVYGGLDTHLEMRGCRVIGNEANYVG
                     GGIYLLDSNSSPRAVTRESIIADSRIVGNHSLAELGGAGIYVDQTSHVALSDVQFVIN
                     TSGSASTVAGQDLLDESRYRADAPDLSGVKFADNVLFFDPKLRNRKDAIPSLISGVLN
                     VLQPASAGKEVFSTVAQPVDFGELRFARQRTTRAQVLGEPAVVAAKVLPSNCFEVQTT
                     ERSVDVIVVHHTSAIKWESLRKEFPDQVREFETRQGQMPPEARKYDWRHCLRIFELYG
                     VSAHYLIDRNGVIRQLVDENDVAYHAGVSKMPPPDGRERVNDFSIGIELIASHPEDDP
                     AVSRSTAYTPRQYQSLHWLLRDICLRRPAISVENILGHEDIAPGRKKDPGPHFDWPRV
                     RQDLSDDLDAFSRMSMAH"
BASE COUNT          793 a         1283 c         1360 g          844 t
ORIGIN      
        1 tcggtcacgc tggccgagag tccgccgccg ccgtcggggt cgggagtggt gacgctttcg
       61 aggaggccga tgagcgtgta ggtgtaactc gtgacgttcg agagtggatc ttcggacgtg
      121 agcaggttgc cggtggaaag ctcgtaggtg ttctcccagg tcttgcccag ctcgttggtg
      181 taggtgaggg gcaggtgcca ggtgctgtgg taggtccagg tctgggagtt gccgtctggc
      241 aggtaggctt tcacctggtc gcccgcggcg ctatagccga gcttggtgat cgggctggtc
      301 agcgccccgc cgccgtcagg atcggcctgc gtgatgcgga ttggtcggcc gtcgtgatcg
      361 cgttccgtcg tggtgacgtt gctcagcgga tcctggaaat gaatgacgtt gccgaaacga
      421 tcagtctgga atttccaggt gttgctacgc tcgtcgatgt actgagcgac ggcatcggcg
      481 ggatcgtaca ggctgttgcc ggtgccgctg gcccagccca tcgtctgctg gggcgtgagc
      541 tgccaggtgg tggagttggg atgggtgatc gtttccaggc gcagcgtgtc ggtgtcgtag
      601 gcgaagctgg tcgtgtagtt catcgggtcg gttgagcgca gacgccgagc gccgcgtgac
      661 ccggaggtca acgaaaagaa gaagccgcct caattactca gccacagcgc caaagagcgg
      721 cgcaatggtc gagatcccta gcgatcaacg gccacgcgaa gcccgcaagc gtgcgcggcc
      781 agggacgacg tgttggcctt ccgggcctag ccagccactc tgttcctgcg ggcgagaaaa
      841 gcaggtttcc cgcgaggctg gcagtcgaaa cgtgcggatt gtccgcggtg agaaaccgct
      901 gtcaagtagg ggagcggggt tctgccgcta ttttgcggga tgccgcaaac cggcgctgcg
      961 aggcggtaaa gcttccacga agaaatgggg aagccggagt cgcgccagcg cagcgtcgcg
     1021 gctactcttg attccccaga aaccaccgcc gcttataaac ggccagtaaa gtcctgcccg
     1081 tcagcttcgc cgcctccgcc ggcggtttgg tccgcacgat ctggtccagc ttcggagtcc
     1141 acctgcgcga aatgcctagg acataccgtc tcgacacgac ggcgctccac gtccgacccg
     1201 tcaacctgac gacctcggcc ggccgcttgg tcctgacgag ccgatcctcc ttggaggacc
     1261 agggcgggtt ctgacccggt ggccgcgtgc cgcgctttcg atgggtggcg ctcatcttct
     1321 tgcgcgtggc ctcgggaagc ggcttgccga gatgcgcctt ccgcatcgtc tcgataacgt
     1381 gtggcggccg ggctacgccc cgcttggcct tggcgatctt ccgcgcccgc gcggggtccg
     1441 tggcccggat cgactcggac cttcgtaggc gagtgccttt ggtgcgtttg acgcgcagcg
     1501 ccttccgcca tctgctgacc agactagcgc ccactcccca ggccacagcc acatcgcagt
     1561 tggcttcttg cttgaccgcc cgcaccaagt cgccggtcac gaccggaata cgatgccccc
     1621 agacgacgcc cagcggccac tgcaccggcg cgtcgctgat ggcaaccacc cgcagcgtgc
     1681 cggcatactc gcagtcgagc ttcccgccga tcttgcagcg gggcatgcgg taggggccga
     1741 agcgaaggcc ggtcatacgg acatgattgc cagcgatgcg atcttcggcc agactggggg
     1801 ccatgccgaa ggtcgtattt caggccgttc tcacggaaac tggcacgccg ccgaaggaat
     1861 ctccgaccga gagtatcgaa ggtgccataa caacggccct ggagaaacaa ctgccggagg
     1921 agttgaggga gatatttgag ctcgactcga aggtgaaggt caataagatt caccgcggaa
     1981 gctactggat cgagttcaca gtcaccttcg gcaccatctt cgccatctat gtggggatct
     2041 ccaggtacaa gaacttcgtg gagagcgtga aactcatcgc ggcgcatgcg caggcggtac
     2101 tcagcgctgc cacgaaggcg tacgggaagt tctcggtttc ggttaggccc attcagggaa
     2161 tgtggcccct ggcacgccgc ctgttttatg cagagttctg gtgggtggtg ctcttgatcg
     2221 aattgtcgat catcgctttg ttgactatcg cgctcttggt gaaacaactt ctggcgtagc
     2281 actgttgact aattgggctt gggagcttcc gggcggaagc tgccgccaag cttcagccgg
     2341 tcagcctcgc atcagtgcgc catcgacatc cgactgaagg catccagatc gtcgctgaga
     2401 tcctgccgga ctcgtggcca gtcgaagtgt ggtccaggat cttttttccg ccccggagcg
     2461 atgtcctcgt ggccgagaat attttcgact gaaatcgcag gccgccggag gcagatgtct
     2521 cgcaggagcc agtgcagact ttgatattgg cgcggagtat acgcggtgct tcgcgaaacc
     2581 gccgggtcgt cctcgggatg ggatgcaatc aactcgattc cgattgagaa atcattgacg
     2641 cgctctcggc cgtcaggcgg cggcatcttg ctgactcccg cgtgatatgc aacgtcgttc
     2701 tcgtccacaa gctggcgaat tacaccgttg cgatcgatca gatagtgggc ggagacgcca
     2761 tacagctcga atatccgcaa acagtggcgc caatcatact tacgcgcctc gggcggcatc
     2821 tgaccctgcc gggtttcaaa ctcccgaacc tggtcaggaa actccttgcg aagtgactcc
     2881 catttgatcg ccgacgtgtg atggaccacg atcacatcga cgctcctctc ggtcgtttgc
     2941 acttcaaagc agttcgatgg cagaactttc gcggcgacga ctgccggttc gccaaggacc
     3001 tgcgcgcgcg tcgttcgttg tctggcgaac cttagctcgc cgaaatccac tggctgcgcg
     3061 acggtcgaaa agacttcctt tcccgcggag gcaggctgta gcacgttcag gacgcccgat
     3121 atcaaggacg gaatggcgtc cttgcggttt cgcaacttcg ggtcgaagaa gaggacgtta
     3181 tcggcgaact tgacgcccga caggtccggg gcatcggcgc ggtaacgcga ctcgtcgagc
     3241 aaatcctggc cggcaaccgt tgaagctgaa ccggaagtgt tgatgacaaa ctgcacgtcg
     3301 ctcagggcca cgtgactcgt ctggtccacg tagatgcccg cacctcccaa ctctgcgagc
     3361 gaatgattgc cgacgatccg cgagtcggcg atgatgcttt cgcgtgtgac ggcccgcggc
     3421 gatgaattgg aatcgaggag gtagatacca ccgccgacgt aatttgcctc gttgccaatg
     3481 actcggcacc cgcgcatttc gaggtgcgtg tccaggccgc cgtaaacggc ccccccgctg
     3541 gcgaactcgt tgccgacggt aggcgcacca tcgggagcga gccccgacca gacgatgccc
     3601 gtgcgggtgt tggcgaaccg acagtcggac gcatagagtt cgctaccgtg tgcaaagatg
     3661 gcgcctccac tgaagccact gatgttctct agaaatttgc agcgttcgac gacgagtcgc
     3721 ttcgcgttga tgcagtaaag cgctccgccc tggttggccg cgttccgttt gaactcgcag
     3781 ttcctaaagc tgaccaaggg agccgaaacg agacaaaccg cgccgcccac cgggtgacgg
     3841 gcatccggcg agcgggtcac ggtcttcgcc acgcggttcc agtcgatgtc gtcggccgtt
     3901 cgcggattcg cccgggtgag gcgggttcca tgaccatgca gaaactggca attttcaaag
     3961 gttgaaccgg caaccagcga accatggatg agcaggccgc cctcttgggc ggggtcggct
     4021 ccaaagtcaa agaccaccgg agcagcgtcc ttggcaagac tgccggccgt cacgactccc
     4081 tcgatttcaa gaacgccccg gccctgccag ttcaacctgg ccccgtttcc gatcaccaga
     4141 cgagaacccg caggaacgag ggcatcgccg gtgatgttga ccgtactccc tgccgggatc
     4201 tcgtaaatcc gtcccggttt gcagactacg ttgtcgaggg tcgtcaggct cgttcgagcg
     4261 accagtgtgt catcaaccaa
//
LOCUS       DASUGB010001127         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_124944, whole genome shotgun sequence.
ACCESSION   DASUGB010001127 DASUGB010000000
VERSION     DASUGB010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124944"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1463)
                     /locus_tag="VFV87_21445"
     CDS             complement(402..1463)
                     /locus_tag="VFV87_21445"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5106405.1"
                     /translation="MPSLLFPTSVIGSLPRPRFVKDLIADDCQVQGEQYKRLMGNAIR
                     AAVAMQEAAGLDVITDGEWWRKSYIGVIAELAHGFELSHAADGRPWTVVTEKLSPKSP
                     GFIAQEVRYLKTLTQRQIKATVPAPALLGERMWDPVKSAKAYPKREDFVRDCVPILRR
                     EVELLKDEGVSIIQVDDPHLCLFVDPEVRKQYDDAEQASDFAVDMDNQVVAGISGVKL
                     AVHLCRRAGARARGEADHRGSYDPILRQLARLNVGHITMEFTSPGAGDMSVFNQLPEH
                     VEIGLGCVSCQPGQIDSADTIASRVEMALKHVAPSRITLNPDCGFAPGSAADVSLDEV
                     YTKLKNEVEAAGRLREKYG"
     gene            complement(1481..>2098)
                     /locus_tag="VFV87_21450"
     CDS             complement(1481..>2098)
                     /locus_tag="VFV87_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106406.1"
                     /translation="FIGGYQIDKVKSDYPQFTFRHNADWEHNNILASLFCAADLMDEP
                     FICCYSDILFTADVIQRLAAAKDDTALGVDTAWLARYAERTDHPPDDAEKVTVANGRV
                     TRVHRQIPQSEAYGEYIGVAKFSAAGAARLRDYYRHVQAAFAGRPWREAAVFEKAYKI
                     LLFQEMIESGERFAHVDTPGGYIEVDTQQDFEYARQHWLTKHRGK"
BASE COUNT          390 a          650 c          650 g          408 t
ORIGIN      
        1 gctcgcgcca accctccgag caccatgagg gccagacaag tcccggccac tcggcgacaa
       61 actgccttaa accgcatttc acttctccat ttcgggataa gacatgcaga ctaatcgctg
      121 ggttagatcg tttcgagggg atagtcgcag gcgaggacca gagactttcg agcgaatgcg
      181 tgggttgttt ttcgttcgac gacattgacg aagccgacga agacacgctc aaccgcatcc
      241 tctggcatgc cgctcgcggc tgagaaggga cctatccggc atggtcggtt ctacctgaaa
      301 ctgatgacga cgaaatcgag cgcgagctcg aatccctgaa acgtcccaac gaggttccgg
      361 gccgctggag ttgacgagaa caattctgag gttgtcagcc gctagccgta tttctcccgc
      421 agtcgcccgg ccgcttcgac ttcatttttc agcttcgtat agacctcatc caggctcacg
      481 tcggcagcgc tgccgggagc aaacccacaa tcggggttga gcgtgattcg cgacggcgcc
      541 acgtgcttca gggccatctc gacgcggctg gcgatcgtgt cggccgagtc gatctgtccc
      601 ggctgacagc tcacgcagcc caggccgatc tcgacgtgct ccggcagttg attgaagacc
      661 gacatgtcgc cggcgccggg ggaggtgaac tccatcgtga tgtggccgac attcagccgc
      721 gcgagttggc ggaggatggg gtcgtagctg ccgcggtggt cggcctcgcc gcgggcccgc
      781 gccccggccc gccggcagag gtgcaccgcc agcttgacgc cagaaatgcc ggcgacgacc
      841 tggttgtcca tatcgacggc aaagtcggag gcctgttcag cgtcgtcgta ttgcttacgc
      901 acctccggat cgacgaacag gcagaggtgc ggatcgtcga cctggatgat cgagacccct
      961 tcgtccttca gcagctccac ctcgcggcgg agaatcggca cgcagtcgcg gacgaagtct
     1021 tcacgcttgg gataagcctt ggcggacttc accgggtccc acatccgctc gcccaggagc
     1081 gccggggcgg gaacagtcgc cttaatctgc ctctgcgtga gggtcttgag gtaacggact
     1141 tcttgggcga tgaagccagg gcttttcggc gagagcttct ctgtgacgac cgtccagggc
     1201 cggccgtccg cagcgtgact cagctcgaag ccgtgagcca actcggcaat cacgccgatg
     1261 tagctcttcc gccaccattc gccgtcggtg atgacatcca ggccggcggc ctcctgcatg
     1321 gcaaccgcag cccgaatggc attgcccatc aggcgcttgt attgctcgcc ttggacctgg
     1381 cagtcgtcgg caatcaagtc cttcacaaac cgcggccgcg gcagcgagcc gatgacggag
     1441 gtggggaaga ggaggctggg catggcgatt tccctcgatg ctattttcct cgatgttttg
     1501 tgagccagtg ctgccgggcg tattcgaaat cctgctgcgt atcgacctcg atgtagcccc
     1561 cgggcgtatc gacgtgggcg aaccgctcgc cggattcgat catctcctgg aaaagcagta
     1621 ttttgtaggc cttctcgaag accgccgctt cgcgccacgg gcggccggcg aaagccgcct
     1681 ggacatggcg atagtagtcg cgcagccggg ccgcgcctgc ggcggagaat tttgccacgc
     1741 cgatgtactc gccgtaggcc tcgctctggg gaatctgccg atgaacgcgg gtcacgcggc
     1801 cgttggcaac cgtcaccttc tcagcgtcgt ccggcggatg atcggtgcgc tccgcatagc
     1861 gcgccagcca ggccgtatcg acgccgagcg ccgtgtcgtc cttcgctgcc gccagccgct
     1921 ggatcacgtc ggctgtaaac aggatatcgc tatagcagca gatgaacggc tcatccatta
     1981 aatccgcagc acaaaacagc gacgccagga tgttattgtg ttcccaatct gcattgtggc
     2041 ggaacgtgaa ctggggatag tcggacttga ccttatcgat ctggtagccg ccgatgaa
//
LOCUS       DASUGB010001128         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_960801, whole genome shotgun sequence.
ACCESSION   DASUGB010001128 DASUGB010000000
VERSION     DASUGB010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960801"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1776
                     /locus_tag="VFV87_21455"
     CDS             352..1776
                     /locus_tag="VFV87_21455"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5106407.1"
                     /translation="MSDFRIEKDSMGEVRVPAQAYYSAQTQRAVENFPISGWHLPRPL
                     IHALGLVKLAAATANRDLGKLTQTGKNKLSDQQVEALLAACREVAQGKFDDQFPIDVF
                     QTGSGTSSNMNTNEVIANRAIELLGGDRFQKEKAVHPNDHVNMGQSTNDMFPTAIHVA
                     VAAAIQSDLIPALEQLGDVLTQKANAWDRVIKIGRTHLADATPLRLGQEFSGLARQIE
                     LSVGRAKKGIEPILDLPAGGPAVGTGINTHPEFGRRVAQALAKETGIPFIEAVNHFEG
                     NAQRDGLVECHGQLRTIAVTLFNVSNNIRWLGSGPRCGFFEVKLPDRQPGSSIMPGKV
                     NPVMCESMMQVAARVIGNDQTLAFSGATGGQFQLNIMMPAMGHAALESVALLASSCRA
                     FVEFCARDMEANPEACEAAVEQSLAMVTSLNPHIGYERAAALAKEAFKSGKTIRQLCR
                     EQGVLPEPTLREALDPWSMTEPRG"
     gene            complement(1874..2572)
                     /gene="deoC"
                     /locus_tag="VFV87_21460"
     CDS             complement(1874..2572)
                     /gene="deoC"
                     /locus_tag="VFV87_21460"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX5106408.1"
                     /translation="MLNCSYASIAKMIDHSLLTPTLAACDLEAGCQLALEYDVASVCI
                     LPYYLRRCADLLRGSTVRASTTIGFPHGGHTTAVKLAEARQALADGGQELDMVVNISQ
                     VLSGAWDYVAQDIAAVVRAAHDAGAQVKVIFENCYLRDEHKIRLCEICGELRADWVKT
                     STGYGAGGATLDDLALMRKHAPPHVQVKAAGGVRDLDTLLKVRELGVTRCGASRTKEM
                     LDELRRGLNVPPIA"
     gene            complement(2587..>2789)
                     /locus_tag="VFV87_21465"
     CDS             complement(2587..>2789)
                     /locus_tag="VFV87_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106409.1"
                     /translation="ARFPYDGANRQQTQARLGYLLKRLPQVVGKESTGDFAEGAEAGN
                     ALSAVSASSAVDYLALWTAAHR"
BASE COUNT          522 a          881 c          921 g          465 t
ORIGIN      
        1 aaggagcgct gcgccgccgg gtttttggct tccgagcaaa catagctcac ccgttttgcg
       61 cagccggcag agatttgctc ggcgggaaag cgccctgggc cggttatgac ggttgtgcct
      121 gccactggcg cctgaggcag cggcagcgct cgcgtccatt gccaaggatg tactgatccg
      181 accaatggga cggatcaggc caatgcgata cgattggccg atagcgaaga gcgaagaacc
      241 aagaaccaag aaccaatcaa acccctaact cctgaccccc gaacccctga cgcctgaccc
      301 ctgaacctcg aacccctgaa ccccgaaccc ctgaaccctg aaccctgaac aatgtccgac
      361 ttccgcatcg aaaaagactc catgggcgaa gtccgcgtcc ccgcccaggc ttattactcc
      421 gcacaaaccc agcgggcggt cgagaatttt ccgatctccg gctggcacct gccgcggcca
      481 ctgattcacg ccctgggcct ggtgaagctg gcggcggcga ccgccaatcg cgacctgggc
      541 aagctgaccc aaaccgggaa gaacaagctc agcgatcagc aagtcgaggc cttgctggcc
      601 gcttgccgcg aggtggccca ggggaaattc gacgaccaat tcccgatcga cgtattccag
      661 accggctcgg gcacgtcgag caacatgaac acgaacgaag tgatcgccaa ccgggcgatc
      721 gagctgttgg ggggcgaccg ctttcagaag gaaaaggcgg tccatcccaa cgaccacgtg
      781 aacatggggc agagcaccaa cgacatgttc cccacggcga tccacgtggc cgtggccgcg
      841 gcgatccaga gcgatttgat cccggctttg gaacagctag gcgatgtgct cacccagaag
      901 gccaacgcct gggatcgtgt catcaagatc ggccgcacgc acctggcgga tgcgacaccc
      961 ttgcggctgg ggcaggagtt tagcgggctg gcgcggcaga tcgagctctc ggtggggcgg
     1021 gcgaaaaagg ggatcgagcc gatcctggac ctgccggctg ggggcccggc ggtggggacg
     1081 ggaatcaata cccaccccga attcggccgc cgcgtggccc aggccctggc caaggaaacg
     1141 ggcatcccgt tcatcgaggc ggtcaaccac ttcgagggca atgcccagcg tgacggtctg
     1201 gtcgaatgtc acgggcaatt gcggacgatc gccgttacgc tcttcaacgt gtcgaataac
     1261 atccgctggc tggggagcgg cccgcggtgc ggcttcttcg aagtgaagct gcccgaccgc
     1321 cagccgggca gctcgatcat gccgggcaag gtgaacccgg tgatgtgcga gagcatgatg
     1381 caggtggcgg cccgcgtgat cggcaacgac cagacgctgg ccttcagcgg ggcgaccggc
     1441 gggcagttcc agctcaacat catgatgccg gcgatggggc acgcggccct ggagagcgtg
     1501 gccctgctgg cctcgtcgtg ccgggcattc gtcgaattct gcgcccgcga catggaggcg
     1561 aatcctgagg cgtgcgaggc cgcggtcgag cagagcctgg cgatggttac cagcctcaac
     1621 ccgcacatcg gctacgaacg ggcggcggcg ctagccaagg aggcgttcaa gtcgggcaag
     1681 acaatccgcc agctgtgccg cgagcagggg gtgctgcccg agccgacgct gcgcgaggcg
     1741 ctcgatccgt ggagcatgac cgagccgcgg gggtgaagtg attgcggatt gcagatttca
     1801 gatttgggat ttggggtttt ttacatacta gtccgaagcg ccagcgagcg agtccgcatt
     1861 ccgcctcgcg gattcaggcg atcggcggca cgttgagtcc ccggcgcagc tcatcgagca
     1921 tttccttcgt gcggctggcg ccgcagcggg tgacgcccag ctcgcgcact ttcagcagcg
     1981 tatcgaggtc gcgcactccg ccggctgctt tgacttgcac gtgcggcggc gcgtgcttcc
     2041 gcatcagcgc caaatcgtcc agcgtggcgc cccccgcgcc gtaaccggtg ctggttttga
     2101 cccagtcggc ccgcagctcg ccgcagattt cgcagaggcg gattttgtgc tcgtcccgga
     2161 ggtagcagtt ctcgaagatg accttcactt gggccccggc gtcgtgagcc gcccggacga
     2221 cggcggcgat gtcctgggcg acgtaatccc acgcgccgct gaggacctgg ctgatgttga
     2281 ccaccatgtc cagctcttgt ccgccatcgg ccagggcctg gcgagcctcg gccagtttga
     2341 cggccgttgt gtggccgccg tgaggaaagc cgatcgtcgt gctggcccgc acggtgctgc
     2401 cgcggagcag gtcggcgcag cggcgcaggt aatacggcag aatgcagacg ctggccacgt
     2461 cgtactccag ggccagctgg cagccggcct ccaggtcgca cgccgccagc gtcggcgtca
     2521 gcagcgagtg atcgatcatt ttggcgatgg atgcgtagga gcagttgagc atgggctgcg
     2581 agtgggctag cgatgagctg cggtccacag agcaaggtaa tcaaccgcgg atgacgcgga
     2641 taccgcggat agagcattgc ccgcctccgc gccctccgcg aaatccccgg tcgattcctt
     2701 tccaacgact tgtggcagcc gtttgagcag gtatcctaag cgcgcttgag tttgctggcg
     2761 atttgcaccg tcgtacggga atcgagccc
//
LOCUS       DASUGB010001129         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_960815, whole genome shotgun sequence.
ACCESSION   DASUGB010001129 DASUGB010000000
VERSION     DASUGB010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960815"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFV87_21470"
     CDS             <1..185
                     /locus_tag="VFV87_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106410.1"
                     /translation="KLSLKSHEQSGHIDFSLDQGRLVSAEQSQKLATERRYRETTIAV
                     TLSSKQTTTMRAKGGE"
     gene            complement(341..685)
                     /locus_tag="VFV87_21475"
     CDS             complement(341..685)
                     /locus_tag="VFV87_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5106411.1"
                     /translation="MTEFTTVAKVTDIPPGQGQAYAVAGRMVAVFNDDGKFFAIDDFC
                     PHMGASLAGGYLADGIVTCPWHAWRFCIHDGRWCDNPRIKIDAFECRVEGDDVQVRVT
                     PKPPPPGAVQAT"
     gene            874..>2848
                     /locus_tag="VFV87_21480"
     CDS             874..>2848
                     /locus_tag="VFV87_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106412.1"
                     /translation="MKSATALLLRGRYDEAAEQYAALASKDPAAAIGVARCRLAVGKS
                     DDAASALATAAGWFPTSAEIQAELALLALARGNEEGAQKQSAGALALDKDCVAAHWVR
                     AELLKHEGRLKEAEQAYAWLTDYYNRAPRIDDLQQLIWIGRGAAEHARWTRNSKQFRR
                     LVSELYPSALRLEKDFWPARLEAAQLYLEKFNQSDAAAEIAAGLAINPQAAELHAARA
                     ALALESFDLATAKSSLDRALEINPRLVWAHQLRADWLLCDVRPAEAIEVLQAGRQLNP
                     RDEATLGRLAAACEAVDGRTGGKHSPRVQAIIDEALSRNPHCGQFYLAAAEAFDRMRR
                     FPQAAEFYRLAGEAMPQLIATRGQLGLVLMRLGDEAAAARLLEESFTIDPFNVRVKNQ
                     LEVLDVLAGYAAIETDHFVVKFDRGQDELLARYAARFLEDEVFPDLSRRLGYAPAGKT
                     LVEIFSQSGNTPGHAWFSARMVGLPFIGTVGACAGKMIALSSPTELKEKYNWATVLRH
                     EMVHVINLQQTDFGVPHWFTEGLAVHLESQPRPRAWAELLARRSRAGTLFTLDDVTLG
                     FVRPKNGEDWTLAYCQSELYIEHLIEKYGQDAPGKMLAAYARQQSTSQALEQLFGVPQ
                     ARFEADYRQKIERTIAAAGPAIARPRPTLADLQR"
BASE COUNT          534 a          904 c          936 g          474 t
ORIGIN      
        1 aaaaactcag cctgaaatcg cacgagcagt ccggccacat cgacttttcg ctggaccagg
       61 ggcggcttgt atccgccgag cagagccaga agctggcgac cgagcgccgc taccgcgaga
      121 caacgatcgc ggtcacgctg agcagcaaac agacgaccac gatgcgggcg aagggagggg
      181 agtaggcgtt cgtcgtcgga gtgtgagggt gtgtgagtgc gagggtgcaa aagtgtgagc
      241 gtgtgagggt gcgagagtag gagagaggcg caagtttgcc cactcttgca cccccatacc
      301 ctaccaccct cacactctcg taccctcact ccctcgcaca tcacgtcgcc tgcactgccc
      361 cgggcggcgg cggcttgggc gttacgcgca cttgcacgtc gtctccttcg acccggcact
      421 cgaaggcgtc gatcttgatc cgcggattgt cgcaccagcg gccgtcgtga atgcagaatc
      481 gccaggcgtg ccacgggcag gtgacgattc catcagccag atagccgccg gccagcgagg
      541 cccccatgtg cgggcagaaa tcgtcgatgg caaagaactt gccgtcgtcg ttgaacacgg
      601 ccaccatgcg cccggcgacc gcataagcct gtccctggcc gggcggaata tcggtcactt
      661 tggcgacggt agtgaactcg gtcatcaggg cgtgtaggct acagggcggc cagtaattgg
      721 gcggcggcgg aaacctcatt gtaaagtccc cccgtggaat cgacagggca ggccggtagg
      781 atgagcgggc agttttctct ggcagggcgc tttgctttgg cggctgcggc aatcgcagcc
      841 gttgcaccgg ctacccgttg tgacgcggcg gacgtgaaat ccgccacggc gttgctgctc
      901 agaggtcggt acgacgaagc ggccgagcaa tacgccgcgc tcgccagtaa agaccctgcg
      961 gcagcaatcg gtgtggcccg ctgccggttg gcggttggca agtctgacga cgcggccagc
     1021 gccctggcga ccgccgccgg ctggttcccc acatcggccg agatccaggc tgaattggct
     1081 ctcctcgcgc ttgctcgcgg caatgaggag ggagcacaaa aacagtccgc cggggctctg
     1141 gcgctcgata aggattgcgt cgccgctcac tgggtgcggg cggaattgct caagcacgag
     1201 gggcggctca aggaagcgga gcaggcgtac gcctggctca cggactatta caaccgcgcg
     1261 ccgcgaatcg acgatctcca gcagctaatc tggatcggtc gcggggccgc cgaacatgcc
     1321 cgctggaccc gcaacagcaa gcagttccgc cgcctggtaa gcgagctgta tccgagcgcc
     1381 ctgcggctgg agaaggattt ttggccggcg cggctggaag cggcgcagct ttatctcgaa
     1441 aaattcaatc agtcggatgc ggcggccgag attgccgccg gcctggcgat caatcctcaa
     1501 gcggccgagc tgcacgcggc ccgggcggcg ctggcactgg aatcattcga cctggccacc
     1561 gcgaaatcgt cgctcgatcg ggccttagaa atcaacccgc ggctagtttg ggcccatcaa
     1621 ctgcgagcag actggctgtt gtgcgatgtt cgtcccgccg aggcgatcga agtgctccag
     1681 gccggccgcc agctcaaccc gcgcgacgag gcaacgctcg gccgcctggc cgccgcctgt
     1741 gaggctgtcg atggccgcac tggcggcaaa cactcgcccc gcgtgcaggc gattatcgac
     1801 gaagcactca gccgcaatcc gcactgcggc cagttctacc tggcggcagc cgaggcgttc
     1861 gaccggatgc ggcgattccc gcaagcagcc gagttttatc gtcttgcggg agaggcgatg
     1921 ccgcagctta tcgcgacgcg cggccagctc gggctggtac tgatgcgcct gggcgacgaa
     1981 gcggcggcgg ccaggctgct cgaagagtcg ttcacgatcg accccttcaa cgtgcgggtg
     2041 aagaatcaat tggaagtgct cgacgtcctg gcgggctatg cggccatcga aaccgaccat
     2101 ttcgtcgtta agttcgaccg cgggcaggac gagctcttgg cccgctatgc cgcccgcttc
     2161 ctggaggacg aagtctttcc cgacctctcg cggcggctgg gctacgcgcc cgcgggcaaa
     2221 acgctcgtcg agattttcag ccagagcggc aatacgccag gccacgcctg gttcagcgcc
     2281 cggatggtcg gcctgccgtt cattggaaca gtcggcgcgt gcgccggcaa aatgatcgcc
     2341 ttgtcgtcgc cgactgagct gaaggaaaag tacaactggg ccacggtctt gcggcacgag
     2401 atggtgcacg tcatcaacct gcagcagacc gactttggcg tgccgcactg gttcaccgag
     2461 gggctggcgg tgcatctgga aagccagccc cgcccgcgcg cctgggccga gctgctcgct
     2521 cgccgcagca gagcgggaac gctgtttacg ctcgatgacg tgacgctcgg cttcgtgcga
     2581 cccaaaaacg gagaagactg gacgctcgcg tattgccagt cggagctata catcgagcat
     2641 ttaatcgaga aatatggcca ggacgccccc ggcaagatgc tcgccgccta tgctcggcag
     2701 caatccacat cgcaggcttt agagcagctg tttggcgtgc cgcaggcccg gtttgaggcc
     2761 gactaccgac aaaaaatcga gcggacaatc gccgcggcag gtccggccat cgcccgcccg
     2821 cggccgacac ttgccgactt gcagcgcc
//
LOCUS       DASUGB010001130         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1237317, whole genome shotgun sequence.
ACCESSION   DASUGB010001130 DASUGB010000000
VERSION     DASUGB010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237317"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VFV87_21485"
     CDS             complement(<1..2108)
                     /locus_tag="VFV87_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106413.1"
                     /translation="MPTVKSIAANFAVLCLGFAAAALLVGCQPAAPPAAGSGGNGTTD
                     GLDITMPTEEPTPTLEESPAPDQPETDKPAEPAPAETPAEEKRAAATPAEEQPAEAKP
                     AEEATSADTKPAAGETALENPALHPGDWTQWGGSSLRNNVPIVENVVTEWNPGKFDRK
                     TGAHDPAKAQNIKWVTPLGTQTYGNTVVAGGKVFVGTNNTYGYLKRYPGDGADAVDLG
                     CLICLDEQTGKFLWQHSSEKLPTGRVNDWPLMGICCSPLVEGDRLWFVTSRGEVRCLD
                     INGFYDGKDDGRPESQEPARLFDVTRADDPAEDKVGGYISELDSGKIPADLRAKFEAA
                     GMPLPDGDIAVTADDKAKAPNKKWAFTAKVGESDRKFILGVAGPKLSAFKIVTPADKE
                     EADVIWVFDMMKTLGTSQHNMCSCSVTTFGDILFVNSSNGVDDSHLVIPAPDAPSFIA
                     MNKNTGEVYWTDNSPGRNILHGQWSSPTVAIIGGVPQVLFGGGDGWLYSFKADTGKNG
                     KPELLWKFDINPKDSVLELGGRGTRNDIISTPVVYDNKVYFCTGQDPEHGEGIGILWC
                     IDPTKRGDISEKLAVNRADPKTPIAHKRVQAVVEADGDIAIDNPNSGVVWKYETNDAN
                     GDGEIDFEEQFHRSIATVAIKNDLLFCPDFSGLFHCVDAQTGKVYWTYDMLAASWGSP
                     LIAGDKVMIGDEDGDVAVFDL"
     gene            complement(2193..4454)
                     /locus_tag="VFV87_21490"
     CDS             complement(2193..4454)
                     /locus_tag="VFV87_21490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106414.1"
                     /translation="MIRLWHGLERTAFALTVVWTSILALPVPTHAQTKPDPLDWPYWR
                     GPEYNNISRETGLPDTIDPDGGEGSNLLWKKTELGGRSTPIVLSGKLYTILRADPGTP
                     KEGEKVVCVDAATGETIWESRHNVWSSEVPDTRVGWSSVVGDPETGRVYALGACGLFE
                     CYEGDTGKIVWSIPMHERFGLLSTYGGRTNFPIIHEDLVILGSVIIGWGDMAVPAHRL
                     IALNKATGDVVWFVSTRLRPPDTIYSAPVLATINGQRLLITGSSDGWVYALQPRTGKI
                     VWEYQFSRRGLNVSPTVDGDIVYTGHSEENPVGTKMGAVAAIKAGLAGNITESGQIWK
                     ELEVGVGKSSILKIDDRLYCFDDAGKARVLDAKTGEPINTRPYGLGTINFASPVYAEG
                     KIYHVEKNGRWYILTPDRDDGLARYKRGETMGMFPSGDECWASPVVSHGRLYLLTTGA
                     LYCFEDKSKEHGATPRPPQPEETPVAQDEKPAQLQVVPCELLMQPGEKQQLTVRLFNA
                     NGQFLKETPAEFAVEGDGAVSPQGEFTASDTGHAATYVTAKAANLTARSRIRVVPPLP
                     WKFDFENLKDPPITWVGARYRHVLRQMDGSNVMVKVSTIPLGTKSRCSMGQSDLSDYT
                     IQADFKAAVANKLPNAGVIAQGYTLEVSGENEWIKLVSWVPHDKRYFKEIPFQLEPNV
                     WYTMKLRATVADGKAVLQGKVWKRDAKEPADWTIELTDPVPNTHGAPGLFGDATNAEL
                     YLDNVSVTPNSVN"
BASE COUNT          809 a         1409 c         1473 g          963 t
ORIGIN      
        1 aggtcgaaga ccgccacgtc gccgtcctca tcgccgatca tcaccttgtc gcccgcgatc
       61 agcggtgagc cccaggaggc ggcgagcatg tcgtacgtcc aatagacctt gccggtctgg
      121 gcatcgacgc agtggaacag accgctgaaa tccgggcaga agagcagatc gttcttgatc
      181 gcgaccgtcg cgatcgagcg gtggaattgc tcctcgaagt cgatctcgcc gtcgccgttg
      241 gcgtcgttcg tttcatattt ccataccaca cccgaattcg ggttatcgat ggcaatgtcg
      301 ccgtcggctt ccaccacggc ctgcacgcgc ttgtgtgcga tgggggtctt cgggtcggcg
      361 cgattcacgg caagtttctc gctgatgtcg ccgcgcttgg tcgggtcgat gcaccacaga
      421 atgccgatcc cttcgccgtg ctcgggatcc tgtcccgtgc agaaatagac cttgttgtca
      481 tagaccacgg gcgtgctgat gatgtcgttc cgcgttccgc gtccgccgag ttccaggacc
      541 gagtccttgg gattgatgtc gaacttccag agcagctctg gtttgccgtt cttccccgtg
      601 tcggccttga agctgtagag ccagccatcg ccgccgccga agagcacctg cgggacgcca
      661 ccgatgatcg cgacggtggg gctggaccac tggccgtgaa ggatgttcct gcccggggaa
      721 ttgtcggtcc aataaacttc gccggtgttc ttattcatag cgatgaagct gggagcgtcc
      781 ggcgcgggga tgaccaggtg cgaatcatcg acgccattgg acgaattgac gaacaggatg
      841 tcgccaaacg tcgtcaccga gcaactgcac atgttgtgct gcgacgtgcc cagcgttttc
      901 atcatgtcaa agacccagat cacgtcggct tcttccttgt cggcgggggt cacgatcttg
      961 aatgccgaca gcttgggacc cgcgacgccg agaataaatt tgcggtccga ttcgccgact
     1021 ttggcggtaa aggcccattt cttgttcggg gccttggcct tgtcgtcggc tgtgacggcg
     1081 atgtcgccat cgggaagagg catgccggca gcttcaaact tcgcgcggag gtcggcggga
     1141 atcttcccgc tgtcgagttc cgaaatgtag ccgccgacct tgtcctcagc cggatcgtcg
     1201 gctcgcgtca cgtcgaacag gcgggctggc tcttgcgatt ccggccggcc gtcgtccttg
     1261 ccgtcgtaaa agccgttgat gtccagacaa cggacttctc cgcggctggt gacgaaccac
     1321 aaccggtccc cctcgaccag cggcgagcag cagatgccca tgagcggcca atcgttgacg
     1381 cggccggtgg gcagcttttc gctggagtgc tgccagagga atttgcccgt ctgctcgtcc
     1441 agacagatca aacagccgag atcgacggca tcggcgccat cgccgggata acgcttgagg
     1501 tagccatacg tgttgttggt gcccacgaag accttgcccc ccgcgacgac ggtgttaccg
     1561 taggtctgcg tgcccagcgg cgtgacccac ttgatattct gggccttggc agggtcgtga
     1621 gcgcccgttt tgcggtcgaa cttgcccgga ttccattcag tcacgacatt ttcgacgatc
     1681 ggcacgttat tgcggagcga actgccgccc cattgagtcc agtcgccggg atggagggcc
     1741 gggttttcga gagcggtctc gccggcagcc ggtttggtgt cggcagatgt cgcttcctca
     1801 gccggtttgg cctccgcggg ctgttcctcg gccggggtcg ccgcagcccg cttctcttcg
     1861 gcgggcgttt ccgcaggggc gggctcagca ggcttgtcag tttccggctg gtccggggcg
     1921 ggcgattcct caagcgtcgg cgtcggctct tcagtgggca tcgtgatatc gaggccgtcg
     1981 gtcgttccgt tgccgccgct tccggctgct ggaggggccg cgggctggca gccgacgaga
     2041 agcgccgcgg cagcgaagcc gaggcacagt acggcgaagt tcgccgcgat ggatttaacg
     2101 gttggcatcg aaagtctccg tcaatcgata ggtcgctgaa cccgtcagaa tatagtcttc
     2161 cgaactctga caagcccggc tacagggaaa tgctagttca cagaattggg ggtaacgctg
     2221 acattgtcga gatatagttc ggcgttggtg gcatcgccaa acaagccggg cgccccgtgc
     2281 gtgttgggca ccgggtcggt cagttcgatc gtccaatcgg cgggctcctt ggcgtcgcgc
     2341 ttccagacct tgccttgcag caccgccttg ccgtcggcca ccgtcgcccg cagcttcatg
     2401 gtgtaccaga cgttcggctc aagctggaac gggatttctt tgaagtaccg tttgtcgtgc
     2461 ggtacccagc ttacgagctt gatccactcg ttctcgccgg aaacttccag cgtgtagccc
     2521 tgggcgatga cgccggcatt cggcagcttg ttggcgaccg ccgccttgaa atcggcctgg
     2581 atcgtatagt ccgacaagtc gctttgcccc atcgagcagc gactcttggt gccgagggga
     2641 atggtggaga ccttcaccat gacgttgctg ccgtccatct gccgcagcac gtggcgatag
     2701 cgggcgccaa cccacgtgat cggcggatcc ttgaggttct cgaaatcgaa tttccacggc
     2761 aacggcggga cgacgcggat acgggagcgg gccgtgagat tggctgcttt cgccgtgacg
     2821 tacgtcgctg catgacctgt atcggaagcg gtgaattcac cctgcggact gacggcgccg
     2881 tcgccttcaa cggcaaattc agcgggcgtc tccttcagga actggccgtt cgcgttgaat
     2941 aaacgtacgg tgagctgctg tttttctccc ggctgcatga gcagctcgca ggggacgacc
     3001 tggagctgag ccggcttctc atcctgggca acgggcgtct cttccggctg cggcggacgc
     3061 ggcgtcgcgc cgtgttcttt cgacttgtcc tcgaagcagt aaagcgcgcc ggtcgtcagc
     3121 agatacagcc ggccgtgcga aacgacgggc gaggcccagc attcgtcgcc ggaagggaac
     3181 atacccatcg tctcgccgcg cttgtagcgg gccaggccgt cgtcgcgatc gggtgtcaga
     3241 atgtaccagc ggccgttctt ttcgacgtgg taaatcttgc cttcggcata gacgggcgac
     3301 gcgaaattga tcgtccctag gccatacggc cgcgtgttga tcggctcgcc ggtcttggcg
     3361 tcgaggacgc gggctttgcc ggcatcgtcg aagcaataca agcggtcgtc gatcttcagg
     3421 atcgaactct tgccgacgcc cacttcgagc tctttccaga tctggcccga ctcggtgata
     3481 ttgcccgcga ggccggcttt gatcgcggcg acggcgccca tcttcgtgcc taccgggttc
     3541 tcctcgctgt ggcccgtata gacgatgtcg ccatcgaccg tgggcgagac attcagtccg
     3601 cgccgcgaga actggtattc ccagacgatt ttgccggtcc gcggctgcag ggcataaacc
     3661 caaccgtcgc tggagccagt gatcagcaac cgctggccgt tgatcgtcgc caggacgggg
     3721 gcggaataga tcgtatccgg cggtcgcagg cgcgtactga cgaaccagac gacgtcgccg
     3781 gtcgctttat tgagggcaat gaggcggtga gccggcacgg ccatatcgcc ccagccgata
     3841 attaccgagc caaggatgac caggtcttca tggatgatcg ggaaattggt tcggccgccg
     3901 taggtggaaa gcaggccgaa tcgctcgtgc atcggaatcg accacacgat cttgcccgtg
     3961 tcaccttcat aacattcgaa gaggccacag gcccccaggg catagacgcg gcccgtttcc
     4021 ggatctccga cgacgctcga ccagccgacg cgcgtatcgg gcacttcgct cgaccaaaca
     4081 ttgtggcgcg attcccaaat ggtctcgccg gtggccgcat cgacgcaaac gaccttttcg
     4141 ccttctttcg gcgtgcctgg gtcggcccgc aggatcgtat acagcttgcc gctgagcaca
     4201 atcggcgtgc tccgcccgcc taattcggtc tttttccaga gcaggttgct cccttcgccc
     4261 ccatccgggt cgatcgtgtc aggtaatccc gtctcgcggc tgatgttgtt gtattccggc
     4321 ccgcgccaat acggccaatc gagcgggtcg ggtttggttt gtgcgtgggt cgggaccgga
     4381 agcgcgagaa tactcgtcca aacaacagtt agggcgaacg cggtccgttc gaggccatgc
     4441 cagaggcgta tcatggtcgg ctcgcgaagg ttggggaggg cgtcagaggg ccgcaactta
     4501 cggccggttc agcaaacggg tcgcggcgct gccgcaagga caggaaggtg cttataggat
     4561 acggcaagcg gccacaggaa gcaagcgaac tgagccaact cgcagcccct caggggtcag
     4621 aatgccgcaa ctagccaaag gggtttcccg aatc
//
LOCUS       DASUGB010001131         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1656300, whole genome shotgun sequence.
ACCESSION   DASUGB010001131 DASUGB010000000
VERSION     DASUGB010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656300"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFV87_21495"
     CDS             <1..447
                     /locus_tag="VFV87_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106415.1"
                     /translation="AEALELAAAPFGPEGLEQLYKLESSPRWRAWYDLTRGRQLAMSV
                     RYAEYRALVETLCRPGGLPDDVNCLQIAPAPRLLLGSVTEARAREAQRLLTRCLEENP
                     NTPWAYLAQRELDHPLGVVHRAWYQEPPPPSPTISVPQAPVVLPKL"
     gene            complement(678..2333)
                     /locus_tag="VFV87_21500"
     CDS             complement(678..2333)
                     /locus_tag="VFV87_21500"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5106416.1"
                     /translation="MLPLYQQQLAALRAAFPSPVSDPVHDGYVVFSLLRTLDRVDALK
                     SQAPILGTPREPDYEAAAEARLAGPSQALEAVIPQLVHVLDGMLIFGHPRSQVNVVAH
                     PSIASIIGTVLPSMYNPNLCSDESGFGFSEAEVRVASMVSRLVGYDPAAAGGVFTFGG
                     TGTLLYGLKIGLEKALPGAFDKGLAGQAVVLCSEAGHYACASASGWLGIGQDNVIRVP
                     ALADNSIDVEALEAAAREQLRAGKAIAAIVATMGTTDAFGIDDLASLHAIRESLVREF
                     QLPYRPHLHADAVIGWAWSVFDDYDFAANPLGFRGRTVRALASAASRIRHLPLADSIG
                     IDFHKTGFAPYISSLFLLKDRASFGEVARDRAAMPYLYHTGQYHPGMFTLETSRSGTG
                     VMAALANLLLLGKDGFRTLIGHAVEMAEVLRELIISRPELSVLNEDNFGPVTLFRAYP
                     PGIDTFRVKDRELNDADYLPELRRHNELNRRIFQRVAASALAGQGVAIGFTEKYRTSV
                     TGEPICALKSYVLSPFADEEQMHAVVRHVLEASEEVETASGGA"
     gene            complement(2394..>3092)
                     /locus_tag="VFV87_21505"
     CDS             complement(2394..>3092)
                     /locus_tag="VFV87_21505"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5106417.1"
                     /translation="VVFHSEASGLPAPSSYLFDSEGRFLAGLRSADAARADLPRIVTD
                     QLKPLSGRPRAYAGRGIAFLTSLPRCRLVIVGGGHVGKAVADLAAELEFDVWIVDDRA
                     EFVAADRFPKAERRISGPIERVLPDLEITGDTYCLIVTRGHNHDQEALFHLCDRGARY
                     VGLIGSRRKIKLIFENLTAAGVSHEALAKVHAPLGIDIGSQTVPEIAVSICAELVAHR
                     NLGQVPGRPRGVEV"
BASE COUNT          567 a         1056 c          984 g          485 t
ORIGIN      
        1 gccgaggcgc tggagctggc agcggcgccg tttggcccgg agggcctgga gcagctttac
       61 aaactggaat cgtcgccgcg ctggcgggcg tggtacgacc tgactcgcgg ccggcagctc
      121 gcgatgagcg ttcgctatgc cgaataccgg gcgctggtcg aaactctctg ccgcccgggc
      181 ggcctgcccg acgacgtgaa ctgcctgcag atcgcccccg cgccgcgctt gctcctcggc
      241 tcggtcacgg aagctcgcgc ccgcgaagcc cagcggctgc tcacgcgctg cctggaagaa
      301 aacccgaata cgccctgggc ttatctcgcc cagcgcgaac tcgatcatcc gctcggcgtg
      361 gtccaccgcg cctggtacca ggagccgccg ccccccagcc ccaccatctc cgtcccgcaa
      421 gcccccgtcg tgctgccgaa gctgtagcgc gacatggcgt cgaaccgccc cggctgccac
      481 tcgcgcttcg ctccgccgcg gagcgggaat gctcgaggcc cgaagggccg gcagtcagta
      541 gccccggaag tgtcggaggc ccgaacggcc gacacttggc attgaggagg cgggtcggaa
      601 gtggcgtcgc gactatgact tgcggcgcgg ggcccccaaa aaaaaggcca ggctctcgcg
      661 gaatatacat ttggatgtca cgcgcccccg cttgccgtct ccacttcttc gctggcctcc
      721 agcacgtgcc gcacgaccgc gtgcatctgc tcttcgtcag cgaacgggga gagcacgtac
      781 gatttgagcg cacagatcgg ctcgccggtc accgacgtgc ggtacttttc cgtaaacccg
      841 atcgccacac cctggccggc cagcgcgctg gccgccacgc gctgaaaaat ccgccggttc
      901 agctcgttgt gccgccgcag ctcgggcagg taatctgcgt cgttcagctc gcggtctttc
      961 acgcgaaacg tgtcgatccc cggcggatag gcccgaaaca gcgtcaccgg cccgaaattg
     1021 tcctcattga ggacggacag ctccggccgg ctgatgatca gctcgcgcag cacttcggcc
     1081 atctcgaccg cgtggccgat cagcgtccga aagccgtcct tccccagcaa cagcaggttc
     1141 gcgagagccg ccatcacgcc cgtccccgag cgggacgtct ccagcgtgaa catgcccgga
     1201 tggtattgcc ccgtgtgata cagatacggc atcgcggcgc gatcgcgggc cacttcgcca
     1261 aagctcgcgc ggtccttcag caagaacagc gacgagatat aaggcgcgaa accggttttg
     1321 tgaaaatcga ttccgatcga atcggccagc ggcagatggc ggatgcggct ggcggcggat
     1381 gccagcgccc ggacggttcg tccgcgaaag cccagcggat tggccgcgaa gtcgtagtcg
     1441 tcgaacaccg accacgccca gccgatgact gcatccgcgt gcaaatgggg ccgatagggg
     1501 agttggaatt ccctgacgag cgactcgcga atcgcgtgca agctcgccag gtcgtcgatc
     1561 ccgaaagcat ccgtcgtgcc catcgtggct acgatcgcgg caatcgcttt tccggcgcga
     1621 agctgctcgc gggcagcggc ttccagggcc tcgacgtcga tcgagttgtc ggccagcgcc
     1681 ggaacgcgaa tgacgttatc ctgcccgatg cccagccagc cgctggcgct cgcacatgca
     1741 tagtgtcccg cttccgaaca gaggaccacc gcctggccgg cgaggccctt atcgaacgct
     1801 ccaggcaggg ccttctccag gccgattttc aggccataga gcagcgtgcc ggtgccgccg
     1861 aacgtaaaca cgcctcccgc cgccgccgga tcgtagccca ccagtcgcga aaccatcgac
     1921 gccacgcgga cctccgcctc cgaaaagccg aatccgcttt cgtcgctgca caggttgggg
     1981 ttgtacatcg acggcaagac ggtgccgatg atgctggcga tcgagggatg ggcgacgacg
     2041 ttcacctggc tccgcggatg gccaaaaatg agcatgccgt ccagcacgtg gacaagctgc
     2101 ggaatcactg cctccagcgc ctgcgacgga ccggccaacc gcgcctcagc cgccgcctcg
     2161 taatccggct cgcggggcgt gccgagaatc ggcgcctgcg atttcagcgc atcgacgcgg
     2221 tccagcgtgc gcagcagcga aaacacgacg tagccgtcgt gcaccggatc ggaaaccggc
     2281 gacggaaacg cggcccgcag ggccgcgagc tgttgctgat agagaggcaa catgaagacc
     2341 atgtcagatt atatcgccgc acatcagagg agaaattcga ggagtctctt tggctaaact
     2401 tcaacgcctc tgggccgccc cggcacctgg ccgagattgc gatgagccac cagctccgcg
     2461 cagatgctca cggcgatctc cggcacggtt tgcgaaccga tgtcgatccc gagcggggca
     2521 tggaccttgg ccagggcttc atgcgagacg cctgcggccg tgagattctc gaaaatcagc
     2581 ttgatctttc gccggctgcc gatcaggccg acataccgag cgccgcgatc gcagaggtga
     2641 aagagcgcct cctggtcgtg gttgtggccg cgggtcacga tcaggcagta ggtgtcgccc
     2701 gtaatctcca ggtcggggag cacgcgctcg atcggtccgc tgatgcgccg ctcggctttg
     2761 ggaaatcgat ccgcggcgac gaattcggcc cggtcatcga caatccacac gtcaaattcc
     2821 aattcagccg ccagatcggc gaccgctttg ccgacgtgtc ccccgcccac gatcaccagc
     2881 cggcagcgcg gcagcgacgt gagaaacgcg atgccgcggc ccgcataggc ccgcggccgc
     2941 ccagacagcg gcttgagctg atcggtgacg atgcgcggca ggtcggcacg cgcggcatcg
     3001 gccgaccgaa gcccggcgag gaagcggcct tcgctatcaa acaggtagct ggacggcgcc
     3061 gggagcccgc tggcttcgga atggaagacg ac
//
LOCUS       DASUGB010001132         4931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1514548, whole genome shotgun sequence.
ACCESSION   DASUGB010001132 DASUGB010000000
VERSION     DASUGB010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514548"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..617
                     /locus_tag="VFV87_21510"
     CDS             132..617
                     /locus_tag="VFV87_21510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEX5106418.1"
                     /translation="MKGRKPKPTLLKLLDGNPGKRPLNEREPVALQGLPAPPQWLDAE
                     AKAEWERIIPDLAEMGVLSRADRPALAAYCTAWSRWVEAEGMVKKFGMIVKSPEKGFP
                     MKSPYLSIADQALETSQNRPLPSFFTWTPVFAVLVVLGAAFFAFAAFVVVFFLDMVEL
                     L"
     gene            complement(628..691)
                     /locus_tag="VFV87_21515"
     ncRNA           complement(628..691)
                     /ncRNA_class="hammerhead_ribozyme"
                     /locus_tag="VFV87_21515"
                     /product="hammerhead ribozyme type II"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02276"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02276"
     gene            complement(692..937)
                     /locus_tag="VFV87_21520"
     CDS             complement(692..937)
                     /locus_tag="VFV87_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106419.1"
                     /translation="MNAERKVIDAAQQALVGRTVVRVRYMEPAEVEALGWFQRGVVLV
                     FDDGTAAYTACDEEGNDAGVLMVQKKRGELCLGRFPV"
     gene            complement(962..1180)
                     /locus_tag="VFV87_21525"
     CDS             complement(962..1180)
                     /locus_tag="VFV87_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106420.1"
                     /translation="MNAPRKRKRRRLTERSLERKLVNLFDTLFSRRSGGWAESFEDAG
                     VLTGNRGVVISVGTGQEFQLTIVESKRR"
     gene            complement(1208..1531)
                     /locus_tag="VFV87_21530"
     CDS             complement(1208..1531)
                     /locus_tag="VFV87_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106421.1"
                     /translation="MTTNPEDLDLGFDLEITSTTRRAAGNGTWVSGTLHGHRFEALVF
                     PDHADNPEYEIGDSRISKLWIQRLADRRQVFNWDRGADVPAADQMAACIVDFLCGGLA
                     DHIYA"
     gene            1767..4568
                     /locus_tag="VFV87_21535"
     CDS             1767..4568
                     /locus_tag="VFV87_21535"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX5106422.1"
                     /translation="MTSVFLSYARADDGEPFDPSVSFVAKLHRDLVSQGLKVWFDRES
                     MPSRNLTFHQEIRDALSTCDRLVLVMGPRAATSEYVRQEWQYAWFEAEKVVTPILRRG
                     DYSLIPDELKRLHCEDFRNDADYAFHLQQLGRILNEPSPPLGKLICVPPLPPQYLSRS
                     DVLEPVRKALRTGLDDSSPFGGTVLQPDQGPLGASKVVGLYGMGGIGKSVLANLLVRD
                     RKIREVFPDGIVWIPLGSQGDLRLHLQEVCRSLGDSGKLANEGEGKARLKQLVKDKAV
                     LLVLDNVWHADALNAFDMLGPRCRALVTTRDSGLLFSVGAQCFDVELLTNSDAALLLA
                     LSAGLDVDSLSAEGLAVLSECGRLPLALTLCGGMMRGGRSWSEILVQLRQARIDRIAT
                     RHSVEPHHTSVWQTIHVSVEMLPPDERARFLELGVFVADETPEAAITTLWSHSGGLSE
                     WDAQNLLLKLGQVSLVQLTGNVHGSDRQRRVRLHDLVSDYVRRRVGDFTPWHLQLLEA
                     YRSKGGGEWSAVPDDGYFRRHIPTHLARACEWSELWRLAADSRWDLLTRWTQGGESRQ
                     GLECLAGLFDFQDRLGRDKMAQAGLATQIARIYTVQGELAESERWLQRSLALAADWRG
                     RRIRAIANHELGSIYLYRGSRQKARDAYSQALQDSLLPPSANDEAAANLLALATIELI
                     EYRPADAKDLAKRAYDLASQDDDSAHRVAALRTMGIAERDLMEMASASGSLLTALEMA
                     VAFQLRQEEVACRNALAWYKYQAAALSNSQPAEASQLFQLNGRQAEQTMNFPYFIEST
                     IGLVWCALLRSDLPEVDSLLAAFPKPEDGPEVPDNLIAAYRVASAASAQLRRSNSDAV
                     SLYHVAAVYAHEHAYHTREADALAGLGACYFQIGQPSDAEHWWRTAISVADSCPPMRR
                     ALVMATIERNRSGESVAPL"
     gene            complement(4616..>4931)
                     /locus_tag="VFV87_21540"
     CDS             complement(4616..>4931)
                     /locus_tag="VFV87_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106423.1"
                     /translation="MAGPVKVKAEDLRKLSDEHLFAVVHVAKRVRLMGQRDLIPLAIA
                     VALNQPEAAGSLFCATDKQIEQTLTSIRNQAVAKARAMQFSSSKPYDGGGPAGSRITR
                     QR"
BASE COUNT          911 a         1448 c         1540 g         1032 t
ORIGIN      
        1 cagagagcgc gtacgtaaag cgttcggcac ggggaggggg accgattttg tgcggaagaa
       61 aagagcgtcg tccggtcgcg tcccgccgcg cgcgccgccg cgaaattggg taaggggggt
      121 gcagaattgg aatgaagggc cgaaaaccca agccgacgct cctcaagctg ctggacggca
      181 accccggcaa gcgcccgctc aacgagcgcg aacctgttgc cctgcaagga cttccagcac
      241 ctccccagtg gctggacgcc gaggcgaagg cggaatggga acgcattatt cctgacttgg
      301 ccgagatggg cgtcctctcc cgagccgacc gtccggcgct ggccgcttac tgcaccgcct
      361 ggagccgctg ggtcgaggcc gaggggatgg tgaagaagtt cggcatgatc gtgaagagcc
      421 ccgagaaggg ctttccgatg aaatccccct atctttccat cgccgatcag gcgctggaga
      481 cgagccagaa ccggcccttg ccgtctttct tcacctggac gccggtcttc gcggtcttgg
      541 tcgtcttggg ggcggccttc ttcgcgttcg cggccttcgt ggtggtcttc ttcttggaca
      601 tggtcgaact cctgtgaaac gtgaacggta tggctgccgt catcaggccg cggggaccac
      661 ccgcgacgac gcccgctcgg gcgtttcggc ttcagaccgg gaagcgaccg aggcacaatt
      721 cgccgcgctt cttctggacc atcaacaccc cggcgtcgtt gccctcctcg tcgcaggcgg
      781 tataggcggc ggtcccgtcg tcaaagacca ggactacccc tcgctgaaac cagccgagcg
      841 cttcgacctc ggcgggttcc atgtagcgga cgcgaaccac ggtgcggcct acgagcgctt
      901 gctgcgctgc gtcgataact ttgcgttctg cgttcatgct tgtgctccgg gtgttggacg
      961 tttagcggcg cttgctctcc acgatcgtga gttggaactc ctggcccgtg ccgaccgaaa
     1021 tgaccacccc gcggttgccg gtcagcaccc cggcgtcctc gaagctctcg gcccagcccc
     1081 cgctgcggcg cgagaagagc gtgtcgaaaa gattgacgag cttgcgttcc aggcttcgtt
     1141 cggtcaggcg gcggcgtttg cgtttgcgag gggcgttcat cgttcgttct ccgtggtagg
     1201 ggttgggtta ggcgtagatg tggtcggcga gcccgccgca caggaaatcg acaatgcagg
     1261 cggccatctg gtcggcggca ggaacgtccg cgccgcggtc ccaattgaaa acctgccggc
     1321 ggtcggccaa gcgctgaatc caaagtttcg agatgcggct gtccccgatt tcgtattccg
     1381 ggttgtcggc gtggtccggg aagaccaggg cctcgaagcg atgcccgtgc agtgttccgc
     1441 tgacccaggt tccgttcccg gcggcgcgcc gcgtggtgct ggtgatttcg aggtcgaatc
     1501 ccaggtcgag gtcttcgggg ttcgtggtca tcgttcgttc tccgttgttc gtggttggcg
     1561 tccgcgacca tcgcgttcgc gtgggacaca gttacctcgt ctttcgcccg tcatccagcg
     1621 cttaacggga caattcctga gggaatctgc agcgggaaat gcagcacggc gctggtgttt
     1681 cgactgaatt cgttgacctt gcgcgtcgaa gggtcctacc atgggcttgc ggggggttca
     1741 tttgggccgg ccgccggagg cttggcatga cgagcgtgtt cctttcctat gcacgcgccg
     1801 atgacggtga gccattcgac ccctcggtgt cgttcgtcgc caaactccat cgcgatctgg
     1861 tttcacaagg gcttaaagtc tggtttgatc gagaaagcat gccatcgcgt aacctgacgt
     1921 ttcatcagga gattcgcgat gcgctgtcca catgtgatcg actcgtgctc gttatggggc
     1981 cacgagcggc aacgtcggaa tacgtcagac aagagtggca atatgcctgg ttcgaggcgg
     2041 agaaggtcgt tacacccatt ctgcggcgcg gcgactattc cctcattccc gacgaattga
     2101 agcgtctaca ttgtgaggac tttcgcaacg acgccgacta tgcgttccac cttcagcagc
     2161 ttgggcgaat tctgaatgaa cccagcccgc cacttggcaa actgatctgc gttccccctc
     2221 tgccaccaca gtacttgtca cggagcgatg ttcttgaacc ggtgcgcaag gcgctgcgga
     2281 cgggtcttga cgattcttcg ccgtttggcg ggacggtctt gcagccggat caagggccgc
     2341 tgggagcttc caaggtggtg gggctctacg gcatgggagg catcggcaag agcgtgctgg
     2401 caaacctcct ggtgcgcgac cgcaaaatcc gtgaagtctt tccagacggc atcgtctgga
     2461 tcccgcttgg ctcgcaaggc gatttgcgat tgcacctaca agaggtctgc cgaagcctgg
     2521 gtgacagcgg caaactcgca aatgaaggag aaggcaaggc cagacttaag caactcgtca
     2581 aggacaaagc tgtcctgctg gtgctcgaca acgtctggca cgcagacgcc ttgaatgcct
     2641 tcgacatgct cggtccacgt tgccgggccc tggtcacgac acgagactct ggcctgctgt
     2701 tttcggttgg ggctcaatgc ttcgatgtgg aattgctcac caatagcgac gccgcgctgc
     2761 tgctcgcgct ctcagcgggg ctcgacgtcg attcgctttc cgccgaaggg ttggctgtcc
     2821 tcagcgaatg tgggcggctg ccgctggcgc tcacgctgtg cggcggcatg atgcgtggcg
     2881 gacgaagttg gagtgagatt cttgtccagt tgcgacaggc acgaattgat cggattgcga
     2941 cccggcattc ggtcgagccg caccacacta gcgtctggca gacgatccac gtcagcgtcg
     3001 aaatgttgcc acccgacgag cgggcgcggt ttctcgaatt gggcgtgttt gtggcagatg
     3061 aaacgcccga ggccgcgatt acgacacttt ggtctcattc cggaggtttg agtgaatggg
     3121 atgcccagaa cctgctctta aaactaggac aagtgtcgct cgtccaactg accggcaatg
     3181 tgcatggttc ggacaggcag cgtagggtga ggctgcacga tttggtgtcg gactacgtgc
     3241 ggcgccgggt gggagacttc actccttggc accttcagct gctcgaggca tatcgctcca
     3301 agggtggagg cgagtggtcg gctgtacccg acgacggtta ctttcgccgc cacattccaa
     3361 ctcatctcgc ccgcgcttgc gaatggagtg aactatggcg ccttgcggcg gattcacggt
     3421 gggatttgct gacgcgctgg acgcagggcg gcgaatcacg gcaaggactg gaatgcctcg
     3481 ccgggctgtt tgattttcaa gatcggctgg ggcgggataa gatggcgcaa gcgggattag
     3541 ccacgcagat cgcgcgcatc tacacggttc aaggtgaact tgcagaatca gagcggtggc
     3601 ttcagcggtc cctcgctctt gctgcggatt ggcgaggcag gcgcattcgg gccattgcca
     3661 atcacgaact gggatcgatc tacctttatc gaggatcgcg ccagaaagcg cgagacgcct
     3721 actcccaggc cctccaagac agcctactgc ccccgtctgc caatgacgag gccgcagcga
     3781 atcttcttgc gttggcaacg attgaactca tcgaataccg ccccgccgat gccaaggatc
     3841 tggccaagcg ggcgtatgac ttagcctccc aggacgatga ttcggcccat cgagttgctg
     3901 ccctgcgaac catgggaatc gcagagaggg accttatgga aatggcgtca gcctccggta
     3961 gcttgctgac ggcgctggaa atggcggtcg cattccagtt gcgccaggaa gaagtcgcgt
     4021 gccgcaacgc tctggcttgg tacaagtacc aggcagcggc acttagcaat tctcagccgg
     4081 cagaagcgag tcaacttttc cagctaaacg ggcggcaggc agagcagaca atgaacttcc
     4141 cttatttcat cgaaagcacg attggtctcg tgtggtgcgc cctgcttcgt tcggacttgc
     4201 ccgaagttga ctcgctactt gcagcctttc ctaagcccga ggacggcccg gaggttcccg
     4261 ataacctaat tgcggcatac agagtagcct cggcggcaag tgcccagctt aggcgctcca
     4321 acagtgatgc agtgtctctt tatcacgttg ccgccgtcta tgctcacgaa catgcttatc
     4381 acacccgcga ggccgacgcg ctcgcggggc tcggtgcgtg ttacttccaa atcggccaac
     4441 ctagcgatgc ggaacactgg tggcgaaccg caatcagcgt ggctgattca tgcccaccga
     4501 tgcgcagggc actggtgatg gccacgatcg agcgaaaccg gagtggtgag tcagtggcgc
     4561 cactttgacc gcacgcctga accgtgtcga ttaaagctag ccgaccaatc ttggctcagc
     4621 gctgcctggt aatccttgaa cccgccggcc cacctccgtc gtagggtttc gagctagaga
     4681 actgcatcgc cctggctttt gccacagctt gattgcgaat gctcgtaagt gtctgttcga
     4741 tttgtttgtc agtggcacag aacaaggagc cggccgcctc gggctgattt aatgcgactg
     4801 caatcgcaag gggaattagg tcgcgctgcc ccatcagcct gacgcgtttg gcaacgtgaa
     4861 ccaccgcaaa caggtgctcg tccgatagct tgcgaagatc ttctgctttt accttcacag
     4921 gacctgccat c
//
LOCUS       DASUGB010001133         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_544306, whole genome shotgun sequence.
ACCESSION   DASUGB010001133 DASUGB010000000
VERSION     DASUGB010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544306"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFV87_21545"
     CDS             complement(<1..343)
                     /locus_tag="VFV87_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5106424.1"
                     /translation="MQRVAVLGLGRFGMNLARQLERNRVEVIAVDRNHRLVDEVKDDV
                     TLAVTLDSTDAEALASHEIHETDVCVVAIGENFEASLLTATALKQLGAKKVIVRAQSA
                     THAEIFRRLGAD"
     gene            complement(518..>2261)
                     /locus_tag="VFV87_21550"
     CDS             complement(518..>2261)
                     /locus_tag="VFV87_21550"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5106425.1"
                     /translation="VALVVATWLACASSVGGGGDSPAEFDPAVYARRAGERKGEAAKG
                     KLLVADVKRTQCLNCHAIQGMESGQSPTGSQQAVATSGMGTSAAGPDLSNVGGKLARE
                     HLIESLLEPSRQIVEGYRQVSLRLADGRVLVGLPAGEEGGRLVLIDSRGERITVPLEE
                     IQERHVALLSLMPERLMSAISPDEFCDLVAFLQTLRAAGQSSPGSGVGGPISLPPRFV
                     RETIASGLTGATALATAADGRVFVCEQTGSLRVIKDDNLLDEPFVRLPVDDAWERGLI
                     GVALDPRFDENGYVYVCYVAREPYPHHRISRFEAAGDQAKPGSEVVLLEGDDQTKLGG
                     KVPAGHQGGALHFGPDGCLYIALGEQTAESPAQRLDSLLGKVLRIDADGTIPNGNPFV
                     GQTTGKYRAIWARGCRNPFTFAFDPASGRMLINDVGGKAEEINEGGAGRNYGWPAAEH
                     GPAKDQQFTGPVHWYPTASICGGAFCPLSPLSPRGRGAGVEGQNHAFPEHYRGKYFFA
                     DFVLGTIRVLDASSNERPIPAETFATGLARPVDLAFGADGSLYVLVRDAWVKDQKFAA
                     GTSSLHRIRPRQAE"
BASE COUNT          375 a          843 c          659 g          384 t
ORIGIN      
        1 cgtcggcgcc caggcggcgg aagatctcgg cgtgcgtggc cgattgcgcc cgcacgatca
       61 ccttcttggc cccaagctgc ttgagcgccg tcgccgtcaa gagcgaggct tcgaaattct
      121 cgccgatcgc cacgacgcac acatccgtct cgtgaatctc gtggctggcc agcgcctccg
      181 cgtcggtcga gtcgagcgtg acagccagcg tcacatcgtc cttcacctca tccacgaggc
      241 gatggttgcg gtccaccgca atcacctcca cccggttccg ctccaattgc cgggccaggt
      301 tcatcccaaa ccgccccagc cccagcactg caacgcgttg cacggctttg tttgctcctt
      361 cgcctggcgt ccgttcgaac gagggtcatc gtcgtcgctc catcgcccca gcggcgttcc
      421 cgcgggcata tagggtagca gaaataggtc ccacgaccct ggggagtaag gcgcgatttt
      481 tcgcagccag ccgccgctcg ttattcgaat gcacccgtca ttccgcctgt cgcggccgga
      541 tgcggtggag cgaactggtg ccggcggcaa acttctggtc cttcacccag gcgtcccgca
      601 cgaggacgta cagcgatcca tccgccccaa acgccagatc gaccggccgc gctaaaccgg
      661 tggcaaacgt ctctgccgga atgggccgct cgttggaact ggcgtccagc acgcggatcg
      721 tccccagcac gaaatcggca aagaagtact tgccgcggta gtgctcgggg aaggcatggt
      781 tttggccctc aaccccggcc cctctcccgc gtggcgagag gggagaaaga gggcaaaacg
      841 ccccgccgca aatcgacgcc gttggatacc agtgaacggg cccggtgaat tgctggtcct
      901 tcgccggccc atgctcggcc gccggccagc cgtaattccg ccccgcgccc ccttcgttga
      961 tctcctccgc cttgccaccc acgtcgttga tcagcatccg tccgcttgct gggtcgaagg
     1021 cgaacgtgaa tggattgcgg cagccgcggg cccagatcgc ccggtatttg ccggtcgtct
     1081 ggccgacgaa cgggttgcca ttgggaatcg tgccgtcggc gtcaatgcgc aggactttgc
     1141 caagcagcga atcgagccgc tgcgcgggcg attcggctgt ctgctcgccg agggcgatat
     1201 acaggcagcc gtccgggccg aagtgaagcg ctccgccctg atgccccgcg ggcaccttgc
     1261 cccccagctt cgtctggtcg tccccttcca gcagcaccac ctcgctgccg ggcttcgcct
     1321 ggtcgccggc cgcttcgaat cggctgatgc ggtgatgcgg atagggctcc ctggccacgt
     1381 agcacacata gacgtagccg ttctcgtcaa accgcggatc gagggccacg ccgatcagcc
     1441 cccgctccca cgcatcatcg accggcagcc gcacgaaggg ttcgtccagc agattgtcgt
     1501 ccttgatcac ccgcagcgag ccggtctgct cgcacacgaa aacgcggccg tccgccgccg
     1561 tggcgagcgc ggtcgcaccg gtcagcccgc tggcgatcgt ctcgcgcaca aagcgggggg
     1621 gcagcgagat cggcccgccc acgccgctgc ccggcgacga ctggcccgcg gcccgcagcg
     1681 tctgcaaaaa ggcgaccaga tcgcagaact cgtcgggcga gattgccgac atcagccgct
     1741 cgggcatcag cgacaacagg gcgacgtgcc gctcttggat ttcctccagc ggcacggtta
     1801 tccgctcgcc gcgactgtcg atcagcacca gccgcccgcc ttcttccccc gccggcagcc
     1861 cgaccagcac gcgcccgtcc gccagccgca agctcacctg ccggtatccc tcgacaattt
     1921 gccgcgacgg ctccagaagc gactcgatca gatgctcccg cgcgagcttc ccgccgacat
     1981 tcgacaagtc gggcccggcg gcggacgttc ccattccgct tgtcgcaact gcctgctgcg
     2041 atcctgtcgg actttgcccg ctctccatcc cctgaatcgc atgacagttc aagcactgcg
     2101 tccgcttcac atccgccacc agcagctttc cctttgccgc ctcccccttc cgctcccccg
     2161 cgcgccgcgc atacaccgct ggatcgaatt cagccggcga gtcaccaccg ccacccacgc
     2221 ttgatgcgca tgccaaccaa gttgcaacaa ccaacgcgac g
//
LOCUS       DASUGB010001134         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1514934, whole genome shotgun sequence.
ACCESSION   DASUGB010001134 DASUGB010000000
VERSION     DASUGB010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514934"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VFV87_21555"
     CDS             <1..1369
                     /locus_tag="VFV87_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106426.1"
                     /translation="AQVFLFVRPAELIASSEGEKVLQALGPALADERTKWEAASGFKL
                     EEIEELKIGLHNNDAKFPRVSFVVKTKEPHSVDELLAKWGNPAAAKEGSSTYYTGPAW
                     AYYVSNSPEDERTFAMGEARDVKEVAKVAGAPPVLIRDVERLRRMTDADRHFTVLFYP
                     QFLFNDDGEPLFAAERNKIRQPLSWLLGDHLQAAAVSLHFGEELYFEMRMLGSLDKEP
                     YQLAAELKDRLNQVPRSLEDYFVLLTPPPYWKKLAFRYPGMVRDLHGNLRIGVENEQA
                     VVNSILPAAAAHNLVLGGELLVATAPGAAVVTTAPAKEPPKTIEEALQLKSTYQFASQ
                     SVEFAMRDLVSDVRDMAKGAPFEFDVKLMGADMEKDGITRNQTIRDFDQKDKTIAEIL
                     TALVMKANPITTVKDPSEADQKLIWVIGPDPVNAGKTIILITTRAAAAANKYTLPAPF
                     VPKNT"
     gene            1441..>3567
                     /locus_tag="VFV87_21560"
     CDS             1441..>3567
                     /locus_tag="VFV87_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106427.1"
                     /translation="MDLFSISCTTCKSRLKVREESAIGQILACPKCGGMVMVNAPEGW
                     QKGAPLPEARPSATPSITTVVEVRRPDETLGDSNFEAIDDLLSDAPPKMKPSSVSVAP
                     DAPGLARPRFVGAPPPVASAASATPAAPLVGEGSAIRKANEAAPSPAAESIAEGPKPP
                     PPGEANAIEPPPPDWSPPQPWRWWAMMVGSVAAGIVMALAVVVGMIRFFRDDPQTIAQ
                     AGSNPPVAGPITPTVSGGNPAATPATSNPSAPVTPETTASAEDTKPPAALPAPMPEEQ
                     PMPPVPMPGAEEGDPLGLARPPKPPDGGANLNDPLAKFDRLIGAEGDPLPMPMETEEP
                     AAATPDPPTADDKPVLPRPAPREVDVAARLADPLPGIETSGTPLADFLQIISDLSTIP
                     ITLEPDGLPLVRRTVESPVVLTADEGRNTTVGRALATGLARHGLEYVASDDQLLVRLV
                     EPEKLPLLGYPVKDLAADEQQMADLGEMLQALIEPDSWEASGGEGALAVDATSGKLNI
                     SQRRAVHAQLLIAFEKLRTARKLPLQSTGYDPGLFQLATRSARAKARLETPITLNFSQ
                     PTPLVRIVDYLGKAGGVRILIDWRDIAGAGWNPDGEATLLADKQPLSAALDALLDPMD
                     LTWRIVDGRTIQVLTPETHGSRVELEFYSASGLLAADATGGALLAKVRAALGEGHFRE
                     TGGPGELHYDAPSQCLLAALPQPQQRE"
BASE COUNT          661 a         1185 c         1159 g          562 t
ORIGIN      
        1 ggcgcaagtg tttctgtttg tccgcccggc ggaactgatc gccagcagcg aaggggaaaa
       61 agtgctgcaa gcgcttggcc ctgccctcgc ggacgagcgc acgaagtggg aggcagccag
      121 cggcttcaaa ctcgaagaga tcgaggagct caagatcggc ctgcacaaca atgatgcgaa
      181 gtttccgcgc gtcagtttcg tcgtgaagac gaaagagccg cactccgtcg atgagctgct
      241 ggccaagtgg ggcaacccgg cagccgccaa ggaaggaagc agtacgtatt acaccgggcc
      301 ggcctgggcc tattacgtgt ctaattcgcc cgaggacgaa cggacgttcg ccatgggcga
      361 ggcccgcgat gtgaaggaag tggccaaggt cgccggcgcg ccgcccgtgc tcattcgcga
      421 cgtcgagcgc ctgcggcgga tgaccgacgc cgatcgccat ttcaccgtcc tcttctaccc
      481 gcagttcctg ttcaacgacg acggcgagcc gctgtttgcc gccgagcgga acaagattcg
      541 ccagccgctc tcgtggctgc tgggcgatca tctgcaggcg gccgcggtga gcctgcattt
      601 tggtgaggag ctgtattttg agatgcggat gctcggctcg cttgacaagg agccgtatca
      661 gcttgctgcg gagctgaagg accgtctgaa ccaggtgccg cggtcgctgg aggactattt
      721 cgtcttgctc acgcccccgc cgtattggaa aaagctcgcg ttccgctatc ccggcatggt
      781 ccgcgacctg cacggcaacc tgcggatcgg cgtcgagaac gaacaggcgg tggtcaacag
      841 cattctgccg gccgcggcgg cccacaatct ggtgctggga ggcgagctgc tcgtggcgac
      901 ggctcccggc gcggcagtcg tgacgaccgc gcccgccaag gagccgccga agacgatcga
      961 agaagcgctg cagctcaaat cgacatatca atttgccagt cagtccgtcg agtttgctat
     1021 gcgcgacctc gtcagcgatg tgcgtgacat ggccaagggc gctcccttcg agttcgacgt
     1081 caaactgatg ggagcggaca tggagaaaga cggcatcaca cgaaatcaaa cgatccgcga
     1141 cttcgatcag aaggataaga cgatcgcgga gatcctcacc gctctggtga tgaaggccaa
     1201 tccgatcacg acggtaaagg acccttccga agccgaccag aaactcattt gggtcatcgg
     1261 tcccgatccc gtcaacgccg gcaagacgat catcctcatc accacccgcg ccgcggcggc
     1321 cgcgaacaag tacacgctcc ccgcgccgtt tgtgccgaag aacacgtgac gattgcagat
     1381 tggggattgc aggtttcaga ttggaatccg caatccgcaa tccgcaatcc gcaatccgcc
     1441 atggatctct tctccatctc ctgcaccacc tgcaaatcgc ggctgaaggt gcgcgaggag
     1501 tcggcgatcg ggcagattct ggcgtgcccc aaatgcggcg ggatggtgat ggtcaacgcc
     1561 cccgagggct ggcagaaagg ggcgccgctg cccgaggcca gaccctcggc gactccctcg
     1621 atcacgacgg tcgtcgaggt ccgccgtccg gacgagacgc tgggcgactc aaattttgag
     1681 gcgatcgacg acctgctctc cgacgcgccg ccgaagatga agccgtcgtc ggtatcggtc
     1741 gctcctgatg caccgggcct tgcccggccg agattcgtcg gcgcgcctcc gccggtcgcc
     1801 agcgcggcct ccgcaactcc cgctgctccc ctcgtgggcg aggggtcggc aatacggaaa
     1861 gcgaacgaag cagcgccatc gcctgcagca gaatccatcg ccgaaggccc caagccgccg
     1921 ccgccgggcg aagccaacgc cattgagccg ccgccccccg attggtcgcc gccgcagccg
     1981 tggcgctggt gggcgatgat ggtcggctcg gtcgcggctg gaatcgtgat ggcgctggcc
     2041 gtcgtcgtgg ggatgattcg atttttccgc gatgatccgc aaacaattgc tcaagccggc
     2101 agcaatccgc cggtcgccgg tccgatcacg ccgactgtca gcggcggcaa tccagccgcc
     2161 acaccggcca cgtccaatcc gtccgcgccg gtaacgccag aaaccactgc cagcgctgag
     2221 gacacaaagc caccagccgc cctgccggcg ccaatgcccg aagaacagcc gatgcctccc
     2281 gtaccgatgc ccggcgcgga ggaaggggac ccgctcggct tggccaggcc tcccaagccg
     2341 ccggacggag gggccaatct caacgatccg ctggcgaagt tcgaccgcct gatcggcgcc
     2401 gagggagatc cgctgccgat gccgatggaa acggaggagc cggctgcggc gacgcccgat
     2461 ccgccgaccg ctgacgacaa gcccgtgctc ccgcggccgg cgccgcggga ggtcgatgtc
     2521 gcggcccgcc tggccgaccc cctgccggga atcgaaacct ccggcacgcc gctggccgac
     2581 ttcctgcaga tcatatcgga cctctcgacg atcccgatta cgctggagcc ggacggcttg
     2641 ccgctggtcc ggcggacggt cgaatcgccc gtcgtactca cggcggacga ggggcgcaat
     2701 acgacggtcg gcagggcgct ggcgaccggc ctcgcgcggc acggcctgga atatgtcgcc
     2761 agcgacgatc agcttctcgt gcggctagtc gagcccgaga agctgccgct cttgggctat
     2821 ccggtcaagg acctggccgc ggacgagcag cagatggccg acctgggcga aatgctgcag
     2881 gcgctcattg agccggattc gtgggaggcc agcggcggcg aaggggcgct ggcggtcgat
     2941 gcgacgagcg gaaagttgaa catctcgcag cgcagggccg ttcatgcaca gcttttaatc
     3001 gccttcgaga agctccgcac ggcccgcaag ttgccgttgc agtccacggg ctacgatcca
     3061 ggcttgttcc agcttgccac gcgctcggcg cgagccaagg cccggctgga gacgccgatc
     3121 acgctcaatt tcagccagcc gacgccgttg gtgcggattg tcgattatct gggaaaggcg
     3181 ggcggcgtgc ggatcctcat tgactggcgg gacatcgccg gtgcaggctg gaaccccgac
     3241 ggcgaggcga cgctgcttgc tgacaagcaa ccgctctccg ccgcgctcga cgcgctctta
     3301 gatccgatgg acctgacttg gcgcatcgtc gatggccgga cgatccaggt cctgacgccc
     3361 gaaacgcatg gcagccgcgt cgagctggag ttctatagcg ccagcggcct cttggccgca
     3421 gatgccacgg gcggcgcgct gctcgccaag gtccgcgccg cgctcggcga aggccacttc
     3481 cgcgaaacag gcggaccggg cgagctgcac tacgacgccc ccagccaatg cctccttgcc
     3541 gctctccccc agccgcagca gcgcgag
//
LOCUS       DASUGB010001135         9606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_404483, whole genome shotgun sequence.
ACCESSION   DASUGB010001135 DASUGB010000000
VERSION     DASUGB010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404483"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFV87_21565"
     CDS             <1..665
                     /locus_tag="VFV87_21565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106428.1"
                     /translation="ASAEKDYARAEKELRTVHELDGAREDATLTLISLLTDQGRPTEA
                     RQIAEKSLERLQATSRVRIKLGEILELQGLSSEAQAQYEKVMSDNQLAGATTDMLDAY
                     NTASARLAGLLADQGINLDQALQLASAAKRYRPEDPLFSDSIGWVHVRKLRAKVGLPY
                     LEAAVNGAPENPVFRYHLGVAYEQMGDFAKARTELTRALASSPNFVGADNARMLLKSI
                     GK"
     gene            870..1097
                     /locus_tag="VFV87_21570"
     CDS             870..1097
                     /locus_tag="VFV87_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106429.1"
                     /translation="MHIEVTSTLTQEDENRIAPAVLKLLSGILEMLPVAYMVRIETAD
                     QHVIEQGNPRVTSWETATAPAGDVTVPFVES"
     gene            1124..2962
                     /locus_tag="VFV87_21575"
     CDS             1124..2962
                     /locus_tag="VFV87_21575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HEX5106430.1"
                     /translation="MCLPLSVPLVSTYHLRLAAIRFAAAVAIILCLPTAARAQSSQNV
                     LLVVNESSPESVTIGDYYAKARSVPSGQIVRLKAPVLESISRETFQTSIETPIAEWLS
                     RYLLQDQILFIVLTKGIPLRVEGTAGQVGTIASVDSELTLLYRKMTGLPVSIAGRVDN
                     PYYLNERPVSEARRFTRSVADIYLVTRLDGFSADDVLKLIDRAGKPVREGRIVLDQKS
                     TMIDRGGDQWLAEAAKRLADAGQAARVTLDATRAVVATTDPVLGYFSWGSNDPANQRR
                     QMGLTYAPGAIGGMFVSTDGRTFREPPATWKPAPAGSASGGQTLAADLIREGITGISA
                     SVAEPYLDAIVRPQILFPAYLSGYTMAEAYYLAMPYLSWQTIIIGDPLCAPFLAAPLP
                     QERLHSGINTELSLPAIFGERRLGATPAPGLNRDAIKLYLKALSLQAQHKPPPEVEAT
                     LERAADMEPRLLAAHVLLAEMATSRGSIDQAITHYRLALQVEPNHLAVLNNLAYALAD
                     KKNAATEALPLAEKAYRLSNQAPIVADTLGWIHFKLDHVATALPLLERAAAALPGEVD
                     VLVHAATVNLASNNMERAKTLLDAAVKANPAAAGRDDVKALRARIK"
     gene            2959..3831
                     /gene="xrtA"
                     /locus_tag="VFV87_21580"
     CDS             2959..3831
                     /gene="xrtA"
                     /locus_tag="VFV87_21580"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEX5106431.1"
                     /translation="MMSRNTIVSIALITAAIVVVFWQVFVRLVDAWIVDGNYSHGFLI
                     IPIAIYFVWERRDKLAAATPEPTWFGLVVLAFGIATLLAGLWGSELFLSRVALIPVIA
                     GIVLFVLGWQHLRILTFPLAFLFLMIPIPAIIFNQIAFPLQLLASRAGAWAISAVGIP
                     VLREGNLLVLAHTTLEVAEACSGIRSLVSLITLGIVYGYFMDSRTWVRALIVASAIPV
                     AIVANAARVAGTGMMAQWIGPEAADGFFHEFSGWIVFLFAFVMILIIQRLITKLAPMP
                     FGPGMSGVPQEGRV"
     gene            3828..4487
                     /locus_tag="VFV87_21585"
     CDS             3828..4487
                     /locus_tag="VFV87_21585"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEX5106432.1"
                     /translation="MSSRARVIVVLAVLAAATVSVARANRAEEVPLRMSFSLFPMQFG
                     EWKGVQRPPFTDRVLEVLGLDDYLTRYYYTANRETVDLYIGYWKSQQQGGAIHSPQNC
                     LPGSGWEPVSQSILSFPDPRTAGAPPLQVNRYVIRKGVERQLVLYWYQSRGRIVGSEY
                     WSKIYLVLDAARYNRTDAALVRLVVPVADGSPEAEARAESQAIGFANAILPQLGAFLP
                     N"
     gene            4542..6779
                     /locus_tag="VFV87_21590"
     CDS             4542..6779
                     /locus_tag="VFV87_21590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106433.1"
                     /translation="MALAVFAACAGPSADEHLAKGNDYFAKSQLPEAILEYKSAVQAD
                     GMRGDVRLKLAEAYVKNGDMPGARREYVAAADLLPNDVNAQVKAGQLLLLGQAFEDAQ
                     SRAKKALAVEPLNVDALVLLGNSLAGLKNLEGAMAEYQEALSLNPGTEAAYANIGAIQ
                     FARGERSAAEATFRKAVDAAPKSVNARLGLGSFLWATERAKEAEDVFKEAIALEPASV
                     AANRALGVLYMSTGRAAEAEPYFKAIAESAQTAEAVTSLADYYITAGRMDDAKGVLVE
                     LAKKPEAFAQATTRLAAVEAVQKDRPRAYELLRAVLDKQPTFVPARLLTLRLLLADSK
                     SDEAMKAAVDLSNDEPGTRAAAEAFVAIGSIEGDRDRTDDAIRAFEDALKVDPRSMAA
                     ALGLARVSLSAGDLNRAETYSRQALALQPQNPVARTLMVRVDLARGNVDRASAELAKL
                     EKEYPNSPTVLNLSATRHLIAGRHEAARAAYTRVAQANPADMEALQGLVSAEVSLGRK
                     KEAVERIDEALKRLPGTARLYMLAARTYAAAGDSQRAEQALKKAIDADPNRLAAYSLL
                     GSFYVSQNRLPDAKDQFQELVKRNPRSVSVNTMLGMILEAQRDIPAAEAQYQKTLAID
                     PEAAVAANNLAWILVSSGRNLQQAQQLATTALKHLPNNPQVNDTLGWAYYQTGRFSEA
                     AKALELSIQHDKTDPTVFYHLGMTYLRLVETAKAKQALETALAMSQDFEGAAEAKRSL
                     SAIKR"
     gene            7008..7589
                     /locus_tag="VFV87_21595"
     CDS             7008..7589
                     /locus_tag="VFV87_21595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX5106434.1"
                     /translation="MKRLIFVAMVAAAMAMAPKAEAALITGGVSFAGGANPTGATDWA
                     TATGVSFINPAITTTASGDYSSVTFGTAVTFNNFSFNPPSTPVSPLWTFNFGGNTYTF
                     DLGLINSVTQGYDVNGNSFLNLSGMGTLNITGFTPTAGSFIFTGNSAGGTFSFSASNA
                     ALPSAVPEPGTMVLFGTGLLGLAGIARRRLAKS"
     gene            7681..>9606
                     /locus_tag="VFV87_21600"
     CDS             7681..>9606
                     /locus_tag="VFV87_21600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106435.1"
                     /translation="MRKVLLVFVLIPLFLGEVACGGDPAATRDAHLRKGDSYVADGKL
                     SEAIVEYKAAVSADQNSGIAHAKLGDALLKTNNLVGAWKELVRAADLLPDDLAAQIKA
                     GQALLSTGQFNDAQARAEKVLERDRRNVDAQIIKGNALAGLKDFDNALIELQSAIESD
                     PTRSAGYGHLGSLQYARGDTEAAEAAFKRGIATEPQSLPLRLALANLYWSTNRPKEAE
                     EALRAALKLDPNDALANKAMAALLLGSGRAAEAEAHLKIVAEKTPTAESQLALADYYA
                     NVQRLPEAKVIYEKVAAGSDQALTAKLRLAALGLSEGDRAGAEKLVDQILAKEPGRVE
                     ALLAKAQIQSSSGRTLEAIETARKATAAGPDSPMAHFVLGVLLKNQYQQADAEQAFKD
                     ALRAVPTFAPANLELAKLALAASRFPDAASYAQAAISSVPGYGEAHLLLARAQMASGN
                     TDAADRSLRSMATQFPNSPAVLAEAGRLRLIKGDRTGGRQLLEQALKQDPKLVSAIEA
                     SIVLDIQDGRKDAARKRLEAAAAAAPADRELQLLAARLYATQFSDLQKAETIARQLIT
                     KDGNDLTAFDTLARVYVERGDLPAATREFENLAAKQPRSVANHTAVGVLYSLQGQTDK
                     AKASFEKVLAIDPTSPLA"
BASE COUNT         1878 a         2989 c         3016 g         1723 t
ORIGIN      
        1 tcgcttccgc ggaaaaggac tacgcccgcg ccgaaaagga actccgcacc gtacacgagc
       61 tcgacggcgc acgagaggac gccacactga cgttgatctc gctgctgacc gatcaaggcc
      121 ggccgacaga ggcgcggcaa atcgcggaaa aatctctgga acgtctccag gcgacctcgc
      181 gtgtccgcat caagctcggc gagatcctgg aactgcaagg cctgtcttcg gaagcccagg
      241 cgcagtacga gaaggtgatg agcgacaacc agctggctgg agcaaccacc gacatgctcg
      301 acgcgtacaa caccgcgtcg gcccgcctgg cgggtctgct ggccgatcag ggcatcaacc
      361 tcgaccaggc actccaactc gcgtcggccg ccaagcgata ccgtcctgag gatccccttt
      421 tttccgacag catcggctgg gtgcacgtcc gcaagctccg agccaaggtc ggccttccgt
      481 acctcgaagc tgccgtgaat ggcgctccag agaatccggt gttccgctat cacctcggag
      541 tggcctacga acagatgggc gacttcgcaa aagcgagaac tgaactgacc agggcgctcg
      601 catccagccc gaacttcgtg ggcgctgaca acgcccggat gctgttgaaa tcgatcggaa
      661 aataggccac aacagatact ggtatgtccc ttgctccggc ggggatggga tgcattccac
      721 caattaccgg aaaccggaat tatcagaatg gatgaaccaa atccccttgg ttgggcagac
      781 cattgtcagc ctttcccgaa acaaggcccg taattggcgt gacagacatg acatggggtg
      841 cggaacgcgg attgctacac atcgttcctg tgcatatcga ggtcacttcg acgctgactc
      901 aggaagacga gaaccgtatc gcgccggcgg tgctcaagct gctctcgggc attctcgaaa
      961 tgctgccggt cgcctacatg gtgcgcatcg agacggccga tcaacacgtc atcgagcagg
     1021 gcaatccgcg cgtgacttcg tgggaaaccg ccacggcgcc ggccggcgac gtcacagtgc
     1081 cgttcgtcga aagctgaggt tcactcctcc gggctggccg ttcgtgtgcc tgccactctc
     1141 cgtcccactc gtttccacat accaccttcg actggccgca atcagattcg ccgcagccgt
     1201 cgcgattatc ctgtgccttc cgacggcagc gcgcgcccag agctcgcaga acgttctcct
     1261 ggtcgtcaac gagtccagcc ctgagtcggt gacgattggc gactactacg cgaaagcgag
     1321 gtcggtgccg tcggggcaga ttgtccggct gaaggcgccc gtcctcgaat caatcagccg
     1381 cgagacattc cagaccagca tcgaaacgcc gattgccgag tggctgtcgc gctatctgct
     1441 gcaggaccag atcctcttca tcgtgctcac gaagggcatt ccgctgcgcg tcgaggggac
     1501 ggccgggcag gtgggcacaa tcgcaagcgt cgactcggag ctgaccctcc tctaccgcaa
     1561 gatgaccgga ctcccggtct cgatcgcggg ccgcgtcgac aatccgtact acctgaacga
     1621 gcgccccgtc tccgaagcac gacggttcac gcgctcggtg gcggacattt acctcgtgac
     1681 gcgtctcgac ggcttcagcg cggacgacgt gctcaagctg atcgatcgag ccggcaaacc
     1741 cgtgcgcgag ggccggattg tcctggatca gaagtcgacg atgatcgatc gcggcggtga
     1801 ccagtggctg gccgaggcgg caaaacggct ggcagatgca gggcaggccg cgcgtgtcac
     1861 gctcgatgca acccgcgcgg tcgtcgcgac cacagacccg gtcctgggct acttctcctg
     1921 gggctcgaac gacccggcca accagcgaag acagatgggg ctcacgtacg cgcccggcgc
     1981 aattggcggc atgttcgtca gcacggacgg ccgcacgttt cgtgagccgc cggccacgtg
     2041 gaagccggcg ccggcgggta gtgcgagcgg cggtcagaca ctggccgccg atctgatccg
     2101 cgaaggcatc accggtattt ccgcgagcgt cgcagagccc tacctcgacg caatcgtccg
     2161 tccccagatt ctgtttcccg cgtatctctc gggttacacg atggccgagg cgtactacct
     2221 cgcgatgccg tatctcagtt ggcagaccat catcatcggc gacccgctgt gcgcgccgtt
     2281 tctcgctgcg ccactgccgc aggagcggct ccactcgggc atcaacactg agctctcgct
     2341 gcccgctatc ttcggagagc ggcggctcgg ggcgactccg gcccccggct tgaaccgcga
     2401 cgccatcaag ctttacctca aggcgctcag tcttcaggcc cagcacaagc cgccgccgga
     2461 ggttgaagcg acgctggagc gggcggccga catggagccg cggctgctgg cggcgcacgt
     2521 cctgctcgct gagatggcca caagccgggg ctcaatcgat caggcgatta cccattaccg
     2581 gctggcgctc caggtcgaac cgaaccacct cgccgtcctg aataatctcg cctacgcttt
     2641 ggccgataag aaaaacgccg caactgaggc acttccgctg gccgaaaagg cataccgttt
     2701 gtcgaatcag gctccaatcg tggccgatac tctgggctgg atacacttca agctcgacca
     2761 tgtggcgacc gcgcttccgc tgctcgagcg agcggcggcg gccctgccgg gcgaggtgga
     2821 cgtgctggtg cacgccgcca ccgtgaatct ggcgtccaac aacatggagc gggcgaaaac
     2881 gctgctggat gcggcggtga aggcaaaccc ggcggcggcc ggccgcgacg atgtcaaggc
     2941 gcttcgggcg agaatcaaat gatgagccga aacaccatcg tttcgatcgc gctgatcacg
     3001 gcggccatcg ttgtcgtgtt ctggcaggtg ttcgtgcggc tcgtcgatgc gtggatcgtc
     3061 gatggcaatt attcgcacgg cttcctgatc attccgatcg cgatctactt cgtctgggaa
     3121 cgccgtgaca agctggcagc ggccacaccc gaaccgacat ggtttggcct cgtggtgctg
     3181 gccttcggca tcgcgacgct gctggcggga ctctggggat cggagctgtt tctgagtcgc
     3241 gtagcactga ttcccgtcat cgcgggcatc gtgctgttcg tgctcggctg gcaacacctg
     3301 cggattctca ccttcccgct ggcgttcctg ttcctgatga ttccgattcc cgcgatcatc
     3361 ttcaatcaga tcgcctttcc gctgcagttg ctcgcgtcgc gcgccggagc gtgggcaatt
     3421 tcagcggtgg ggatccccgt gctgcgtgag ggcaacctgc tggtgctggc gcacacgaca
     3481 ctcgaggtcg ccgaagcgtg cagcggaatt cgatcgctcg tctccctcat cacgcttgga
     3541 attgtgtacg gctatttcat ggattcgcgg acgtgggtac gcgcactgat tgtggcgtca
     3601 gcgatcccgg tcgcgattgt cgccaacgcc gcgcgcgtcg ccggcaccgg gatgatggcg
     3661 cagtggatcg gtccggaagc ggcggacggc ttttttcatg agttctccgg ctggatcgtc
     3721 tttctgttcg cgttcgtcat gattctgatc atccagcggc tgattaccaa gctggcgcca
     3781 atgccctttg gtccgggcat gtcgggagtc ccgcaggaag gacgcgtatg agctcgcgtg
     3841 cgagggtgat cgtggtactg gccgtgctcg ctgcggcgac tgtgtcggtg gcccgggcga
     3901 acagggcgga ggaagtgccg ctgcgcatgt cgttttccct gtttcccatg cagttcggtg
     3961 agtggaaggg ggtgcagcgt cctccattca ccgacagggt tctcgaggtg cttggcctcg
     4021 acgactacct gacgcggtac tactacaccg ctaaccgcga gacagtcgac ttgtacatcg
     4081 ggtactggaa gagccagcag cagggcgggg cgattcactc gccgcagaac tgcctgccgg
     4141 gctcgggctg ggagcccgtc tcccaatcga ttctctcgtt tccggatccg cgcacggcgg
     4201 gcgccccgcc tttgcaagtc aaccgatacg tgatccgcaa aggcgtcgaa cggcagttgg
     4261 tgctttattg gtaccagagc cgcggccgca tcgtcggcag tgagtactgg agcaagatct
     4321 atctcgtcct cgacgcggcg cggtacaacc ggaccgatgc cgcgctcgtc cggctcgtgg
     4381 tgccggtagc ggacggatcg cccgaagccg aagctcgcgc tgagagccag gcgatcgggt
     4441 tcgcgaatgc aatccttcca cagctgggcg cgtttcttcc gaactgaacg gtcccctcag
     4501 gagcgaatat gacaagccgt gtggtcaggg gtctgtccgt cttggcgctg gcggtttttg
     4561 ccgcctgcgc tggaccctcc gcagatgagc atctggccaa aggtaacgac tactttgcaa
     4621 agtcccagct gccggaagcc atcctggaat acaagtccgc cgtccaggcc gacggcatgc
     4681 gcggcgatgt tcgattgaaa ctggcggaag cctacgtgaa gaacggggac atgcccggtg
     4741 ccaggcgcga atatgtcgcg gccgccgatc tgctgccgaa cgatgtgaat gcgcaggtca
     4801 aagccggcca gcttctgctt ctcggacagg cgtttgaaga tgcgcagtcg cgagcgaaga
     4861 aagcactggc ggtggagcca ctgaacgtcg acgcactggt actgctcggc aactcactcg
     4921 cgggcctcaa aaacctcgag ggcgcgatgg ctgaatacca ggaagcgctt tccctcaatc
     4981 cgggaacaga agcggcctat gcgaacatcg gggcgattca gttcgctcga ggcgagaggt
     5041 ccgccgccga ggccacattt cgaaaggccg tcgacgcggc gccgaagtcg gtgaacgcgc
     5101 gactcggact cggaagcttc ctgtgggcga cggaacgcgc gaaagaggcc gaggacgttt
     5161 tcaaggaggc gatcgcactc gaacctgcga gtgtcgccgc caaccgcgcg ctcggcgtgc
     5221 tctacatgtc cacaggccga gccgcggagg ccgagccgta cttcaaagcc atcgccgagt
     5281 cagcgcaaac cgcggaagcg gtgaccagcc tggccgacta ctacatcacc gccggccgga
     5341 tggacgacgc gaaaggcgtg ctcgtcgagc tggcaaaaaa accggaagcg ttcgcacagg
     5401 ccaccactcg gctggctgcg gttgaggcgg tgcagaagga tcggccacgg gcgtacgagc
     5461 tgcttcgagc ggtgctcgac aagcaaccaa cgttcgtccc ggcgcgattg ctcacgctgc
     5521 gcttgctcct ggccgattcg aagagcgatg aggctatgaa ggccgccgtc gatctgtcga
     5581 acgatgagcc tggcactcgc gccgccgcag aggcgttcgt ggcgatcggc tcaatcgaag
     5641 gcgatcgtga ccggaccgat gacgccatca gggcgttcga ggacgcgctc aaagtcgatc
     5701 cgcgatcgat ggcggccgcc ctcggtctgg cgagggtttc gctgagcgcg ggcgatctga
     5761 accgtgcgga gacgtattcg cgacaggcac tcgccctgca gccacagaat ccggtggcga
     5821 gaacgttgat ggtgcgggtc gatcttgccc gtggcaacgt cgatcgcgca tcggcggaac
     5881 tggccaagct cgaaaaggaa tatccgaatt ctccgactgt gttgaacctc tccgcgacga
     5941 gacacctcat cgccggccgg catgaggccg cacgagcggc ctatacgcgg gtggcgcagg
     6001 caaaccccgc cgacatggaa gcactgcagg ggcttgtgtc ggcggaagtc agcctggggc
     6061 gcaagaaaga ggcagtcgag cggattgacg aggcgttgaa acgtctgcct ggaacagcca
     6121 ggctatacat gctggcggcc aggacctatg cggccgctgg cgattcgcaa cgtgctgaac
     6181 aggcgctgaa gaaggcgatc gacgccgatc cgaaccggct ggccgcttac agtttgctgg
     6241 gctccttcta cgtcagccag aaccggcttc cagacgccaa agatcaattt caggagctcg
     6301 tgaagcggaa cccacgatcg gtttcagtca acacgatgct cggcatgatt ctggaggctc
     6361 agcgggacat tcccgcggcc gaggcgcaat atcagaagac cctggcgatc gatcccgagg
     6421 cggccgtcgc ggccaacaac ctcgcgtgga tattggtgtc gtcggggcgg aatctccaac
     6481 aggcgcagca actggccaca acggccctca agcacttgcc gaacaacccc caggtgaatg
     6541 acaccctggg ctgggcctac taccagaccg ggcggttctc agaggcggcc aaggcgctcg
     6601 aactcagcat ccagcacgac aagacggacc cgacggtttt ctaccacctg gggatgacgt
     6661 atttgcggct ggtagagact gctaaagcca agcaggcgtt ggagacagca ctggcaatga
     6721 gccaggattt cgagggcgct gctgaagcga aacgttccct gtcagcgatc aaacgctgat
     6781 accgcgtcta aagtaccttc gcgggctgac tttcgacttt atttcacaat tgtcactaag
     6841 acttaagtga ctcgtttata tggagttatc ggcgaaatta gagatccgtt cttgagggca
     6901 tctttcttgc taactagagg agccagcaat tgccgtaggc gttagaatca ctggccgcgg
     6961 ttagctgaaa cggtatgggc cctacggccc ggaggtggag tgaacttatg aagcgtttga
     7021 ttttcgtagc gatggtggca gcggccatgg ccatggcgcc gaaggccgaa gcggccctga
     7081 tcacgggcgg cgtttccttt gcgggcggtg cgaacccgac aggagctacc gattgggcaa
     7141 cagcaaccgg cgtgagcttt attaatccgg caatcaccac gacggcgtct ggcgattact
     7201 cgagtgtcac gttcggcact gcggtgacgt tcaacaactt ctctttcaac ccgccctcca
     7261 ccccagtgtc gccgctgtgg acgttcaact ttggcggcaa cacctacacg ttcgacttgg
     7321 ggctgattaa ttcggtgaca cagggttacg atgtcaacgg aaactcgttc ctcaatctct
     7381 cgggtatggg aacgctcaac attaccggct tcaccccgac cgcaggctcg ttcattttca
     7441 ccggcaactc tgccggcgga acgttcagct tctcggcctc gaacgcggca cttccgagcg
     7501 cggtcccgga gccgggcacg atggtgctgt tcggcacggg tcttctgggc ctcgcgggca
     7561 tcgcccgtcg tcgtctcgcg aagtcgtaag cgctttcgtt tcaacaacaa gaccgtaatt
     7621 ccagggctcc cggtggcaca aagccgccgg gggccctgtg ctattctggc caaaatttcc
     7681 atgcggaaag tcctcctcgt tttcgttcta atccccctgt tcctgggcga agtggcctgc
     7741 ggcggcgacc cggccgcgac gcgcgatgcg cacttgcgca aaggggattc ctacgtcgca
     7801 gacggcaagc tctcggaagc gatcgtggag tacaaagccg cagtcagcgc ggaccagaac
     7861 tccgggattg ctcacgccaa gctgggtgat gcactcctga aaaccaacaa cctggtcgga
     7921 gcctggaagg aacttgttcg cgccgcagat cttctgcccg acgatctcgc cgcccagata
     7981 aaagccggcc aggctctcct gtcgaccggt cagttcaacg atgcgcaagc tcgcgcggag
     8041 aaggtcctgg agcgggaccg gcggaacgtc gatgcacaaa tcatcaaggg caatgcgctt
     8101 gccggcctca aggatttcga caacgccttg attgaattgc agtcggcgat tgaaagcgat
     8161 cccacccgga gtgccggcta cgggcacctg ggctcattgc agtatgcgcg cggcgatacc
     8221 gaggcggctg aggccgcatt caagcgcggc atcgccaccg agccgcagtc gctgccgctc
     8281 agattggcgc tcgcgaacct ctactggtcg acgaacaggc ccaaggaagc cgaggaggcg
     8341 ctccgggccg cactcaagct ggacccgaac gatgccctgg caaacaaggc gatggcggct
     8401 ttgcttctcg ggtccggacg cgcggccgag gccgaagccc atctgaagat cgttgccgag
     8461 aaaactccca cggccgagtc gcaactcgcg ctcgccgact attacgcgaa cgtgcagcgt
     8521 ctaccggagg ccaaggtcat ttatgagaaa gttgccgccg gtagcgatca ggcgctcacc
     8581 gcaaaactcc ggctcgccgc acttggtttg agtgaaggcg accgcgccgg cgcggaaaaa
     8641 cttgtcgacc agattctcgc gaaggaacct gggagagttg aagcgctgct ggcaaaggcc
     8701 cagatacagt cgagctcggg aagaaccctg gaggcgatcg agacggccag aaaggccacg
     8761 gccgcgggcc ccgatagccc gatggcgcac ttcgtcctgg gagtcctcct caagaatcag
     8821 taccagcagg cagatgcaga gcaggcgttc aaagatgctc tgcgtgccgt gccgacattc
     8881 gcgcccgcga atctcgagct agcaaagctg gctctcgcgg ccagccgatt cccagacgcg
     8941 gcttcgtacg cacaagcggc gatctcgagc gtccccggtt atggcgaggc gcacctgctg
     9001 ctcgcgcgag cgcagatggc aagcggcaac accgacgccg ccgaccggtc gctgaggtcg
     9061 atggcgacac agtttccaaa ctcgcccgca gtgcttgcag aggcgggacg actgcggctc
     9121 atcaagggcg atcggacagg cggtcgacag ttgctggaac aggcgctcaa acaggatccg
     9181 aaattagtca gcgcgatcga agcgtcgatc gttctcgaca ttcaggatgg ccgcaaggat
     9241 gctgcacgca agcgtctgga ggccgcggcg gctgccgcgc ctgctgaccg ggagttgcaa
     9301 ctgctggcgg cgcgcctgta cgccactcag ttctcggacc tgcagaaggc cgaaaccatc
     9361 gcgcgccagt tgatcacgaa agatggcaac gacttgaccg cgttcgacac actcgcgcgt
     9421 gtttacgtgg agcgcggcga ccttcccgct gcgacgcgcg agttcgagaa cctggccgcg
     9481 aagcagccgc gatccgtggc caaccacacg gccgtcgggg ttctttactc cctgcagggc
     9541 cagactgaca aggccaaagc gagcttcgaa aaggtgctgg caatcgatcc gacgtcgccg
     9601 ctcgcg
//
LOCUS       DASUGB010001136         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1237871, whole genome shotgun sequence.
ACCESSION   DASUGB010001136 DASUGB010000000
VERSION     DASUGB010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237871"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFV87_21605"
     CDS             <1..782
                     /locus_tag="VFV87_21605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5106436.1"
                     /translation="GPRDLAQRSFQIKREALAGRRLHVVAPSVWLADQARQSHLLGNA
                     ASIRVIPYGLDTVQFSPTEKALARQQLGLPQDRFVIGFGATSLTQHRKGMTPLLAALA
                     QLERKSDIVCVAFGGGGLPWSGELPEIHSVGFLRDPGKLALVYSAADVFALPSLEDNL
                     PQTGLEALACGTPVVGFATGGIPDFVHPGETGLLARRGDAGDLAAQIQRLRDRADERV
                     AMGSRGRRLVLERFSSEREASQYIALYGELRAERAARPTAA"
     gene            857..1711
                     /locus_tag="VFV87_21610"
     CDS             857..1711
                     /locus_tag="VFV87_21610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5106437.1"
                     /translation="MRAKLTCLIPCKNEAANIEACIESLRPIADEILVADSGSTDETM
                     EIVRQIGRCKLIEREFINYADFKNWAIPQAAHEWVLIVDADERLTPELAAEIRDVLEC
                     PPQSVDGYWIYRRSFFLGHEIKHCGWDTDCVFRLIRRDHCRYGDCRVHEEIFVPQERA
                     GALKAKLLHYTYWSYDQYFAKYLRYTKWGALDMWDAGKRASFSSLLLRPFLRFLQLYI
                     LKLGFLDGLAGIQVCMLQAFFVSFVKQGRLWEMEHARPQPMPPQPCKARSRPMNRPKP
                     REPGAQST"
     gene            1708..>2573
                     /locus_tag="VFV87_21615"
     CDS             1708..>2573
                     /locus_tag="VFV87_21615"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5106438.1"
                     /translation="MTTLAVHPPQVQPTAACPAEILRCPITHGPLRELTPEELDGINT
                     RIERGTIRHVDGSPVAQTLDAGYIGIGGEFAYGVRDRIALLVPALAIQLASASNQNKT
                     DRLRSEKQAVQRYYDDIGWSTGAGGKAFVDAQNWEDLRAVAQDYIRACHRRVKRHLRP
                     TGKYLLDVASGPVQYPEYLEYSERYAVRICVDISFAALREARRKLGDRGIFILGDITN
                     LPLAEGIADGVVSLHTIYHTPADEQATAFREIYRVLAPGCTAAVVYCWRSALIKAVML
                     PAKLVRGPWKLA"
BASE COUNT          466 a          869 c          784 g          454 t
ORIGIN      
        1 gcggtccgcg cgacttggcg cagcgttcgt ttcaaatcaa gcgtgaagcc ctggcgggcc
       61 ggcgcctgca cgtcgtcgcg cccagcgtgt ggctcgctga tcaagcccgc caaagccacc
      121 tgctgggcaa tgcggcgagc attcgcgtga ttccctatgg gctcgacacg gttcagtttt
      181 cgcccacgga aaaggcactt gcccggcagc agctcggcct gccgcaggac cgcttcgtca
      241 tcggctttgg ggccacctcg ctcacccagc atcgcaaggg gatgacgccg ctgctcgcag
      301 cgctcgccca attggagcgc aaatcggaca ttgtttgcgt cgccttcggc ggcggcgggt
      361 tgccctggag cggcgaattg ccagaaatcc actccgtcgg attcttgcgc gatccgggca
      421 agctggcgct cgtttattcg gctgccgacg tgttcgcgct gccgtcgctg gaagacaatc
      481 tgccgcaaac cgggcttgaa gcactcgcct gcggcactcc cgtcgtgggc ttcgcgaccg
      541 gcggaattcc cgacttcgtc catcctggag agacgggact ccttgcgcga cgcggcgatg
      601 caggcgatct ggccgcccag atccagcggc ttcgcgatcg tgcagacgaa cgcgtcgcca
      661 tgggcagccg cggacgccgg ctcgtgctcg agcgattcag ctccgagcgc gaagccagtc
      721 aatacatcgc gctgtacggc gagctgcgcg ctgagcgcgc ggcacgcccg acagcagcgt
      781 gatttttccc ctcctcgcaa caggcacccc aacccgccct gcaattcgcc acccgcaacc
      841 agacaaggaa gtcggcatgc gcgcgaaact cacctgcctg atcccctgca agaacgaagc
      901 ggcgaatatc gaggcctgca tcgagagcct ccgccccatc gccgatgaaa tcctcgtcgc
      961 agactccggc tcgaccgacg agacgatgga aattgtgcgg caaatcggtc gctgcaagct
     1021 gatcgagcgt gaatttatca actacgccga cttcaagaac tgggccattc cccaggccgc
     1081 gcacgagtgg gtgctgatcg tcgatgccga cgaacgcctg acgcccgagc tggccgccga
     1141 aattcgcgac gtgctcgaat gcccaccgca gagcgtcgat ggctattgga tttaccgccg
     1201 cagcttcttc ctgggccatg agatcaagca ctgcgggtgg gacaccgatt gcgtcttccg
     1261 cttgatacgt cgcgatcatt gccgctatgg cgattgtcgc gtccacgagg agatcttcgt
     1321 gccccaggag cgggcaggcg cgctgaaagc gaagctgctg cactacacgt attggtcgta
     1381 cgatcaatac ttcgccaagt atctccgcta caccaaatgg ggcgcgctag atatgtggga
     1441 cgccggcaag cgagccagct tcagcagcct gctcctgcgg cctttcctcc gcttcctgca
     1501 gctttatatc ctcaagctcg gcttcctcga cggcctggcc ggcattcagg tctgcatgct
     1561 gcaggcgttt ttcgtgtcgt tcgtcaagca gggccgcctg tgggagatgg agcacgcccg
     1621 cccgcagccg atgccccctc agccctgcaa ggcccggtcc cgaccaatga accggccaaa
     1681 gccgcgtgag ccaggagctc aatcaacatg acgacccttg cagtccaccc cccgcaagtt
     1741 cagcccacgg cggcatgccc ggctgagatc ctccgctgcc cgatcacgca cggcccgctc
     1801 cgcgaattga cgccggaaga gctcgatgga atcaatacgc gcattgagcg gggcacgatc
     1861 aggcacgtcg atggctcgcc ggtcgcgcaa acgctagacg caggctacat aggcatcggc
     1921 ggcgagtttg cctacggagt tcgcgacagg atcgcgctgc tcgtgccggc cttggcaatc
     1981 cagctcgcgt ccgcgtcaaa ccagaacaag accgatcgcc tccgctccga aaagcaagcc
     2041 gtccagcggt attacgacga catcggctgg tccacgggcg cgggaggcaa ggcgttcgtc
     2101 gatgcccaga actgggaaga cctgcgggcg gtggcgcagg actacatccg tgcctgccac
     2161 cgccgcgtca agcggcatct gcggccgacg ggcaagtacc tgctggatgt ggcctcgggg
     2221 cccgtccaat atccggagta tctggagtac tcagagcgct atgctgtacg gatctgcgtc
     2281 gatatttcat tcgccgccct gcgcgaagcg cggcgcaagc tcggcgatcg cggcatcttc
     2341 atcttgggcg acattacgaa cctgccgctg gcagagggca tcgccgacgg cgtcgtttcg
     2401 ctccatacga tctaccacac gccggccgac gagcaggcga ccgccttccg cgaaatctac
     2461 cgcgtgctgg cccccggctg cacggcggcc gtcgtctatt gctggcgctc ggcactcatc
     2521 aaagcggtca tgctgccggc gaagcttgtc cgtgggccgt ggaagctcgc gca
//
LOCUS       DASUGB010001137         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1237877, whole genome shotgun sequence.
ACCESSION   DASUGB010001137 DASUGB010000000
VERSION     DASUGB010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237877"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..929)
                     /locus_tag="VFV87_21620"
     CDS             complement(195..929)
                     /locus_tag="VFV87_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5106439.1"
                     /translation="MLQPFLKSVTLRREWVESFEVFPYSIPAIRHLHTLELHPRVTFF
                     VGENGTGKSTLLEALAVAEGFNAEGGSRHAMFQTKETHDKDLPRKLWLGKNKVPRNDS
                     FYLRAESFYNLSTYVDQLGFDPVKNPYGAVPLHEQSHGEAFLTLMLQRLTGNGLYLMD
                     EPEAALSPQRQLTFLAALHELVAKGGQFIIATHSPIIMAYPDATILHFTQEGIAPIAY
                     TDTEHYKVTRAFLTRTELMLKELLEP"
     gene            1134..1931
                     /gene="hisF"
                     /locus_tag="VFV87_21625"
     CDS             1134..1931
                     /gene="hisF"
                     /locus_tag="VFV87_21625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5106440.1"
                     /translation="MLAKRVIPCLDVDRGRVVKGTNFLNLRDAGDPVEVARRYEQEGA
                     DELVFLDITASHEGRDIMLDVVRRTAEQVFMPLTVGGGIRAIDDIRALLNAGADKVSI
                     NSAACQNPDFVRQAAQRFGSQCIVVNIDPKRVAAGKGTGDRGQGTGDVVFEVHINGGR
                     ISTGLEAVSWARKVEELGAGEIVLTSMDCDGTKDGYDLEITRAVSESVGIPVVASGGA
                     GKPEHLADAILEGKADAALAASIFHFGEYSIRETKQIMAGRGIPVRL"
     gene            1961..3202
                     /locus_tag="VFV87_21630"
     CDS             1961..3202
                     /locus_tag="VFV87_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5106441.1"
                     /translation="MATAHEQSALDTPDNGAPEEDYSHHSQSDHSHDPGADLLKQRLL
                     TKREELDVDKYFRALVKLEGSDLHMKVGRPPMIRTRNELRPLNRPPIDAEEMMDLLLP
                     MMNERNKKIFYEEGGADFAYTVDVDGVVWRFRVNLLQQLGKIGMVSRRVNNKIPDFKG
                     LYLPPSIEGLCHYDQGMVLLAGVTGSGKSTTIASMLNYINQRERVHILTLEDPIEFVF
                     TEEKAVINQREIGQDVKDFKIGMKHAVREDPDIMLVGEMRDEETFMTAIHAAETGHLV
                     FGTIHASSSPSTIGRILDLFPEEMHKAMRSAIAFNMKGIVAQKLLRSIKPGVPRVPTC
                     EIMLFTPIIRKIVLEAQDNKLADAIRIGAADGMQDFTMSLKQLIDDGLIDRPTAFEVA
                     PNPDALKMALKGINVSQPGMI"
     gene            3202..>4250
                     /locus_tag="VFV87_21635"
     CDS             3202..>4250
                     /locus_tag="VFV87_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106442.1"
                     /translation="MPRSSIVGALRRRSADGPRVLTCVAALLLVLLIGGELLAQEAAW
                     PTYSGGNRGPGFYLALYKIFLLLLVFWMWVKSADWVSRDSLELGDAIGMPAKIWNPIM
                     VFSFLVALVVLGLGIPIFLASFAVVLLAYAVPFVIYVVQRNGKVTEDKMVFTREHLKN
                     WFANIGKSKPKEVDVKHAWMLGPPVELSPVGPLQMENQSALIEARQSPAYVPVKFLLA
                     DALAQRADKIMLDYTADAVNIRYQIDGIWHNALPKVREMKKGEEGPSVDRALGDMMLA
                     VMKRVGHLNMQERRARQEGKMRVEFGGNKFDTTLLSQGTPTGERVVLNFAIVTKHVRT
                     LEDLGMRDKLREQLK"
BASE COUNT          837 a         1292 c         1358 g          763 t
ORIGIN      
        1 tccagctccg cgaccgcgtg gcgctcagcc gggccacaag aagcacgagc aggtcttgat
       61 tcccacgaag cagtgccagc aggttgtgcc ggtaaagccg cggcgctgcc acggttgcgg
      121 ccgcaaacgg tgcggccacg atcccgcgcc ggagcgtgaa cgactaccac gagaaccaac
      181 catttagcga tccgctacgg ctccaacagc tctttgagca tcaattccgt gcgggtgagg
      241 aacgcccgcg tcactttgta gtgctcggtg tcggtgtagg cgatcggcgc gatgccttcc
      301 tgcgtgaagt gcaggatcgt cgcgtcggga taggccatga tgatcggcga atgcgtggcg
      361 atgataaact gcccgccctt ggcgaccagc tcgtgcaggg ccgccagaaa ggtgagctgc
      421 cgctgcgggc tgagggccgc ctccggctcg tccatcaggt acagcccatt gcccgttagc
      481 cgctggagca tcagcgtcag gaacgcctcc ccgtgcgact gctcgtgcag cggcacagcg
      541 ccataagggt tcttcactgg gtcaaatccg agctgatcga cgtacgtcga gagattgtag
      601 aagctctccg cccgcagata gaagctgtcg ttgcggggaa ccttgttctt gcccagccac
      661 agcttccgcg gcaagtcctt gtcgtgcgtc tccttggttt ggaacatcgc gtgacgcgag
      721 cccccttcgg cgttgaagcc ttccgccacc gccagcgcct ccagcagcgt cgatttgccc
      781 gtcccattct cgcccacaaa gaacgtcacg cgcgggtgca gctccagcgt gtgcaggtgc
      841 ctgatcgccg ggatggaata cggaaagacc tcgaacgact ccacccactc gcggcggagc
      901 gtgaccgatt ttagaaacgg ctggagcata taccgtgacc ttccccacga actgcaaaac
      961 gggcttccaa ccagtccggc gtgggcaggt tgaagcactt gtcctattgt cgatcgattt
     1021 gtggtcgttg acccgcttcg gcatgggatt tagagtggat ttttgtcctt tcgcccggtt
     1081 cctggcggct ggcgggcggc tgcctgcctg ctcattgtct atggttcgcg atcatgctcg
     1141 ccaaacgcgt cattccctgt ttggatgtcg atcggggccg ggtcgtgaag ggaaccaact
     1201 tcctgaacct gcgcgacgcg ggcgatccgg tggaagtggc ccgccgatac gagcaggaag
     1261 gggcggacga actggtgttc ttggatatca cagccagcca cgaagggcga gatatcatgc
     1321 tcgacgtcgt ccgccgcaca gccgagcagg tcttcatgcc gctgacggtg ggggggggca
     1381 tccgcgcgat cgacgacatc cgcgcactcc taaatgccgg cgccgacaag gtttcgatca
     1441 attccgccgc ctgccagaac cccgatttcg tccgccaggc cgcccagcgg ttcggcagcc
     1501 agtgcattgt ggtgaatatt gatccgaagc gggtggcggc gggcaagggg acaggggaca
     1561 ggggacaggg gacaggggat gtcgttttcg aggttcacat caatggcggc cgcatctcga
     1621 cgggcctgga ggcggtttcg tgggcccgga aggtcgagga attgggcgcc ggcgagatcg
     1681 tgctgacgag catggattgc gatgggacga aggacggata cgatttggag atcacgcgtg
     1741 cggtgagcga gtcggtgggc attccggtcg tcgccagcgg cggcgccggc aagccggagc
     1801 acctggccga cgcgatcctg gagggcaagg ccgacgccgc tttggccgcc agcatttttc
     1861 actttggcga gtattcgatt cgcgagacga agcagatcat ggccggccgg ggaatcccgg
     1921 tgcggctgta gaacgcagca ggcaaacgga ggagagagcg atggccacgg cccatgaaca
     1981 atcggcactg gatacgcccg acaacggcgc acccgaagaa gactacagcc accacagcca
     2041 gtcggatcac agccacgatc cgggcgctga cctgctcaag cagcgactgc tcacgaagcg
     2101 cgaggagctc gacgtcgata agtacttccg cgcgctagtc aagctggagg gctccgacct
     2161 gcacatgaag gtgggccgtc cgccgatgat ccgcacgcgg aacgagctgc gcccgctaaa
     2221 ccgcccgccg atcgacgcgg aagagatgat ggacctgctg ctgccgatga tgaacgagcg
     2281 gaacaagaaa atcttctacg aggaaggcgg ggccgacttc gcctatacgg tcgatgtgga
     2341 cggcgtcgtc tggcggttcc gcgtcaacct cttgcagcag ctcggcaaga tcggcatggt
     2401 ctcccgccgc gtgaacaaca agattccgga cttcaaaggc ctgtatctgc cgccgtcgat
     2461 cgaagggctc tgtcattacg atcaagggat ggtgctgctc gcgggcgtca ccggctcggg
     2521 caagagcacg acgatcgcct cgatgctcaa ctacatcaac cagcgcgagc gggtccacat
     2581 cctcaccctg gaagacccga tcgaattcgt cttcaccgaa gaaaaggcgg tgatcaacca
     2641 gcgcgaaatc ggccaggacg tcaaggattt caagatcggg atgaagcacg ccgtccgcga
     2701 agacccggac atcatgctcg tcggtgaaat gcgcgatgaa gagactttca tgacggccat
     2761 ccacgccgcc gaaacggggc acctggtgtt cggcacgatc cacgccagca gttccccctc
     2821 cacgatcggc cgtattctcg acctcttccc cgaagaaatg cacaaggcga tgcggagcgc
     2881 catcgccttc aatatgaagg ggatcgtcgc ccagaagctt ttgcggtcga tcaagccggg
     2941 cgtgccccgc gtgccgacct gcgaaatcat gctctttacg ccgatcattc gcaaaatcgt
     3001 gctggaagcc caggacaaca agctggccga cgccattcgc atcggcgctg ccgacggcat
     3061 gcaggatttc accatgagcc tcaagcaact gattgacgac ggcctgatcg accggccgac
     3121 cgcctttgaa gtcgccccca acccggacgc cctcaagatg gcgctcaagg gcatcaacgt
     3181 gtcgcagcct ggaatgatct gatgcctcgt agttccattg tcggcgcact gcgccgtcgt
     3241 tctgcagacg ggccgcgcgt gctgacgtgc gtcgccgcgc tgttgttagt gctgctgatc
     3301 ggcggcgagc tcttggcgca agaagccgcc tggccgacct attccggcgg caaccgcggg
     3361 ccgggcttct acctggcgct ctataagatc ttcttgctgc tgctcgtgtt ctggatgtgg
     3421 gtcaaatcgg ccgactgggt cagccgcgac agcctggaac tgggcgatgc gatcgggatg
     3481 ccggccaaaa tctggaaccc gattatggtg ttctcgttcc tggtggcact cgtcgtctta
     3541 gggctgggca ttcctatctt tctggccagc ttcgcggtcg tgctgctcgc ctatgccgtg
     3601 ccgttcgtca tctatgtcgt gcagcggaac gggaaagtga ccgaagacaa gatggtcttc
     3661 acccgtgagc acctcaaaaa ctggtttgcg aacattggca agagcaagcc gaaggaggtc
     3721 gatgtcaaac acgcctggat gctcggtccg ccggtggagc tcagtccggt cggcccgctg
     3781 cagatggaga atcagtcggc cctgatcgag gcccgccagt cgcccgccta tgtgccggtc
     3841 aagttcctgc tggccgacgc cctggcgcag cgggccgaca agatcatgct ggattacacg
     3901 gcggacgcgg tcaacatccg ctaccagatc gacggcatct ggcacaacgc gctgccgaag
     3961 gtgcgggaaa tgaagaaggg ggaggaaggc ccctcggtcg atcgcgccct gggcgacatg
     4021 atgctggcgg tgatgaagcg cgttggccac ctgaacatgc aggagcgccg cgcccgccag
     4081 gaaggcaaga tgcgggtcga gttcggcggc aacaagttcg acacgacgct gctcagccag
     4141 gggacgccga cgggcgagcg agtcgtgttg aactttgcca tcgtcaccaa gcacgtccgg
     4201 acgctcgaag acctgggcat gcgcgacaag ctgcgcgagc agctcaaaga
//
LOCUS       DASUGB010001138         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_821919, whole genome shotgun sequence.
ACCESSION   DASUGB010001138 DASUGB010000000
VERSION     DASUGB010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821919"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VFV87_21640"
     CDS             complement(<1..1090)
                     /locus_tag="VFV87_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106443.1"
                     /translation="MRQCLAVIVTAIFALTGCERPVAVNQPAASSGVAQAAEPQAVEA
                     TEPASGATAAAAPSEPAEISAETLASTPGPLPPDLGTRKQGVDWPIFLGPTGDSKSPE
                     TGILTKWEAGQPRIVWNAKLGTSYGAPAISRGRLFQFDRFGDTARLYCLNAETGEALW
                     QYEYQTKYEDMYGYNNGPRCSPVVDDDRVYALGVDGALVCVRSEDGKPVWKVDANKQF
                     GVVQNFFGVGSTPVIEGNLLIVMVGGSPEESQRLPPGALDRVIPNGTAIVAFDKFTGR
                     VKYKLGDDLASYASLKLATINGRRWCFAFCREGLLGFEPASGQLDFHYPWRARILESV
                     NASMPVVVDDEVFISETYGPGSTLLKVAP"
     gene            1266..1685
                     /gene="arfB"
                     /locus_tag="VFV87_21645"
     CDS             1266..1685
                     /gene="arfB"
                     /locus_tag="VFV87_21645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5106444.1"
                     /translation="MNLQISPSLTIPDDELRFSFARSSGPGGQNVNKVSSKAVLHFAV
                     AASPTLPDDVRGRFLERYASRITNAGEIVIHSDEFRDQPKNIQSCLDKLREMLLAVLR
                     PPKKRRPTKPTKGSKVRRLKDKKARSQLKAGRRFSGE"
     gene            1871..>2421
                     /locus_tag="VFV87_21650"
     CDS             1871..>2421
                     /locus_tag="VFV87_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106445.1"
                     /translation="MKHSFVPLALAAALALFAGSGCTPQPAAGPTGGETTPAVNTGDG
                     GAEEQIPFKLGDMIEPFTPPTLAELNAKAEWEDRPVLDSFQLLREHLAESKPLVSVEE
                     ALKLRNDGSKAVNEKILSGLGQYPASEDEALWDASINRLAFGDVNSTNPLLISSTIEF
                     DVSGLIGFGLFGFDWNFNNFAAK"
BASE COUNT          476 a          801 c          718 g          426 t
ORIGIN      
        1 ccggtgcgac cttcagcagc gtgctgccgg ggccatacgt ctccgaaatg aagacttcgt
       61 cgtcaacgac gaccggcatg ctggcgttaa cgctctccaa aatccgcgcc cgccacgggt
      121 agtgaaaatc gagctggccg ctggcgggct caaagccgag cagcccctcg cggcagaacg
      181 cgaagcacca gcggcggccg ttgatggttg cgagcttgag cgacgcgtag ctggccagat
      241 cgtcccccag cttgtacttc acgcggccgg tgaacttgtc gaaggctacg atcgccgtgc
      301 cgttgggaat cacgcggtcc aaggcgcccg gcggcagccg ctgcgattct tccgggctac
      361 ccccgaccat cacgatcagc agattcccct cgatgaccgg cgtgctgccg acgccgaaga
      421 aattctgcac cacgccgaac tgtttgttgg catcgacctt ccacaccggt ttgccgtcct
      481 ccgaccttac gcagaccagc gcgccatcga cccccagggc gtacacgcgg tcgtcatcga
      541 ccaccggcga gcaccgcggc ccgttgttgt agccgtacat gtcctcgtac ttggtctggt
      601 actcgtattg ccagagcgcc tcgcccgtct cggcattcag gcaatacagc cgggccgtat
      661 cgccgaaacg gtcgaattga aacaggcggc cgcggctgat tgccggcgcg ccgtagctgg
      721 tgccgagctt tgcgttccac acgatccgcg gctggccggc ttcccatttg gtgagaatgc
      781 ccgtttccgg cgatttactg tcgcccgtcg ggccgaggaa gatcggccaa tcgacgccct
      841 gcttgcgggt tcccaaatcg ggaggcagcg gaccgggcgt gctggccaag gtttcggcgg
      901 atatttcagc cggctcgctc ggcgcggccg cggcagtggc tccacttgcc ggctcggtcg
      961 cttcgacagc ctgcggctcc gcggcttggg cgacgccaga tgatgcagcc ggctgattta
     1021 ccgccacggg tcgctcgcag ccggtcagtg cgaaaatggc cgtcacaatc accgccaggc
     1081 attgccgcat cacgataaac ctctccaaca cattaacccg cagcgtaagc gagggacacc
     1141 aacccgcagc gtaagcgagg gactccctcg tttacactgc gggttggtgc tgggctcgca
     1201 cttctcccca agccgatcga ccgtaatata ggctgttcag acgccatcac caacgcgccg
     1261 cgaccatgaa cctgcagatc agcccgagcc tgacgattcc ggacgatgag ctgcgcttct
     1321 cgttcgcccg cagcagtggc ccgggcgggc aaaacgtcaa caaggtcagc agcaaggcag
     1381 tgctgcactt tgcggtcgcc gcgtcgccaa ccctgccgga cgacgtccgc gggaggttcc
     1441 tcgagcgcta tgccagtcgg atcaccaacg cgggcgaaat cgtcatccac agcgacgaat
     1501 tccgcgatca gccgaagaac attcagtcct gcctcgacaa gcttcgcgag atgctgctgg
     1561 ccgtgcttcg cccgccaaag aagcgccgcc ccaccaagcc gacaaaaggc tccaaggtcc
     1621 gccggctgaa agacaaaaag gcccgctcgc agctcaaagc cgggcggcgc ttctcgggag
     1681 agtgaagccc gcaagttcaa cggccgacag cctttgcgcg cccgactcag gttgccgaac
     1741 gggggcgaaa tccatacgat gaccctagtt agccagtacc gaacggctgc ccaggtgcgg
     1801 ccgtaggaat acctacggaa ttcctgactg caaccttgtg cctcccggct tcacaatatc
     1861 gagtttcgta atgaagcatt ccttcgtccc actcgctctc gccgccgccc tcgccctctt
     1921 cgccggcagc ggctgcactc cccagcccgc cgccggacct accggtggcg aaacaacgcc
     1981 ggcggtcaac accggtgatg ggggggcgga agagcagatt cctttcaagc tgggcgacat
     2041 gattgaaccg ttcacgccgc cgaccctcgc ggagttaaac gccaaagcgg aatgggaaga
     2101 ccgtccagtg ctcgactcct ttcaactgct gcgcgagcat ttggcagaat ccaagcccct
     2161 ggtaagcgtg gaagaggcgt taaagctgcg aaacgacggc agcaaggccg tcaacgagaa
     2221 gatcctgagc ggcttgggcc aatacccagc cagcgaggac gaggcgctgt gggatgccag
     2281 catcaatcgg ctcgcgtttg gcgacgtcaa cagcaccaat ccgttgctca ttagctcgac
     2341 gattgaattc gatgtgagcg gactgatcgg tttcgggttg tttggtttcg actggaattt
     2401 caataacttc gccgccaagg a
//
LOCUS       DASUGB010001139         5152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_961432, whole genome shotgun sequence.
ACCESSION   DASUGB010001139 DASUGB010000000
VERSION     DASUGB010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961432"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1091
                     /locus_tag="VFV87_21655"
     CDS             165..1091
                     /locus_tag="VFV87_21655"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5106446.1"
                     /translation="MSLPSFETDRPTPPPMRKTSGMAIWSLILGAASIVCAFFTGLPA
                     ILLGLLALGRIHGSQGQLTGRWLAIAGTVLGVIGCLLGVVSVFQALSVRRLAIQRMQS
                     SIHIHEIGIAIHNYHDVYNAIPLAGSDDSELGLNMSWRVRVLPFIEQKPLHEQVNYHE
                     PWDSPANSALLERMPKSYAVPPAQGGHETSYMAFTAQQPVQSGDLQGMPFFGNGTGRG
                     QGFASLAACVDGTTNTIMIVEADRDRSAPWMKPVDLPVDPANPKAGLGHVRSGGFIVV
                     MGDGSVRFLPNSIDNSTLLKLILRDDGRTVDF"
     gene            1237..1584
                     /locus_tag="VFV87_21660"
     CDS             1237..1584
                     /locus_tag="VFV87_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106447.1"
                     /translation="MVRKSILPLTWDAPREFRERLGVRCMPRRTVLGLRRIPPRCHNS
                     SAKAAHDQRERKKPKSCGVEFTSQRTTSSQEATTTVRASPPSTWATPPEEKSRFTSAI
                     SVGLTKARQRGGI"
     gene            complement(1581..2480)
                     /locus_tag="VFV87_21665"
     CDS             complement(1581..2480)
                     /locus_tag="VFV87_21665"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5106448.1"
                     /translation="MLQTFNETNRNLLVNINGELIHRDRAGVSPFDSVVQGGDAVWEG
                     LRLYQGRIFRLKEHLDRLRSSALALAFTQIPTHKDIIEQVRRTLAANQMTDDVHIRLT
                     LTRGVKITSGMDPRLNTAGPTLIVLAEHKPPVYDQQGLSLVTSSVRRSPPDCLDPKIH
                     HNNLIQSILAKIEANVAGADDAVMLDTRGFVAETNATHLFLVYRGEVHTPRTVACPEG
                     ITRAVVLEICREQGIPHEVRDLSLTEFYRADEVFCTGTMGGLAAVTKIDGRIVGAGQP
                     GPMTRRLSDQFAEKTKSEGLPIT"
     gene            complement(2571..4118)
                     /locus_tag="VFV87_21670"
     CDS             complement(2571..4118)
                     /locus_tag="VFV87_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5106449.1"
                     /translation="MAKGNDSGLLELLKENGKGGDVWSALAARKPDHDPSDEGAREAD
                     TRLNEMLRRINDLATGDPNKPPQAQNSAAPAAPVQAASAAPAATAKAAAPAPDEFIPI
                     EPATIRDAGLSDSEVESLILKYLLSRGDCTGREVADQIRLPFILLDPLLRQMKFDQLL
                     VYRGSAPMNDYVYQLTDLGRERARRMMEHCTYFGAAPVSLADYVGCVKAQSLEKQHPS
                     EQDLKNAFSDILVNQRMLHRLGPAINSGRGLFLYGSAGNGKTTIAERVTRAFGQFVWI
                     PRAIGIDGEIIRLFDPVNHEEAPLPQDSGLLDQSRIDRRWVRIRRPTIVVGGELQMDN
                     LEVTLNRSTGISEAPLQLKSNCGTLVIDDFGRQKMSIDQLLNRWIVPLEKRYDFLNLP
                     NGKKIHVPFDQLIIFSTNLEPKDLVDEAFLRRIPYKIEIVDPTIEEFKDLFKLMCGRL
                     GFEYDESAINHLIQHFYKKNNRPLRCCQPRDLLMQVKNYCLFRKETPKLSPEYFDLAV
                     ENYFAVM"
     gene            4447..>5152
                     /locus_tag="VFV87_21675"
     CDS             4447..>5152
                     /locus_tag="VFV87_21675"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5106450.1"
                     /translation="MESGSADRSATSDDRRAALERLFLRIGEVATLPTAAQKILTLTE
                     NPDANVDGLRDAIQGDPVLVARLLRRLNSSYYSLSQKVTDLKTAVTLLGTREIRNLAL
                     TVFVSRFYEAKGAHGTYKREDLWAHSVGVGAAARLVARVCGRGAPEEAFIAGLLHDIG
                     LILLDQSLRRHFHRVIEAIDPATPTHIVENRILTFDHAILGGFVARKWRIPEPVTAAI
                     TFHHQPWCYSGPHNDLV"
BASE COUNT          974 a         1520 c         1611 g         1047 t
ORIGIN      
        1 cccgtcgccg tcagcaatcc catcttcatc gacgtcgatg gcggaggctt caccgcgaat
       61 ggcgacctgc tcgacgtgcc gctgccgatc gacccgacga agccgattac gccgaaagtc
      121 gtaatgccgt agacgctggc aacggatatt gcccggagtt ggatatgagt ctgccatcat
      181 ttgaaaccga ccgtcccacg ccgccaccga tgcgcaaaac gagcggcatg gcgatctggt
      241 ccctgatact tggtgcggcg tcgatcgtgt gcgccttttt cactggcctg ccggccattc
      301 tgcttggttt gttggctctg ggtcggattc acggctccca gggccaattg accggccgtt
      361 ggctggcgat cgccggaacc gtgctgggtg tgatcggatg cctgctgggc gtcgtatctg
      421 tttttcaagc cttgtccgtt aggcgactgg caatccagcg aatgcaatcc agtattcaca
      481 tccacgaaat tggtattgcc attcacaact atcacgatgt ctacaacgcg atcccgctgg
      541 ccggctcaga cgattcggaa ctggggctca acatgagttg gcgcgtgcgg gttctgccgt
      601 tcatcgaaca aaaaccgttg catgagcagg tcaattacca cgagccctgg gacagcccag
      661 caaattccgc cttgttggaa aggatgccga agtcgtacgc cgttccgccg gcgcagggcg
      721 gccacgaaac ttcctacatg gcgttcaccg cacaacagcc ggtccagtcg ggcgatcttc
      781 aaggcatgcc cttttttggc aacgggaccg ggcgcgggca gggattcgcc agcctcgcgg
      841 catgcgttga tggcacgacg aacactatca tgattgtcga ggccgaccgc gaccgctccg
      901 caccctggat gaagccggtc gatttgcccg tagatccagc gaatcccaag gcaggtctgg
      961 ggcacgtgcg atctggcggg tttatcgtcg tgatgggcga cgggagcgtg aggtttctcc
     1021 ctaactcgat cgacaactcc acattgctca aattgattct gcgcgacgac ggtcgtacgg
     1081 tcgatttcta acgtccgcgc gcaaatctcg aagccgcagc aacttgcgtc ttcgcgcggc
     1141 cttcacaaag aaccgcctcg cgggagggaa tcaaccggtg ggtaaccccg cctgacttat
     1201 tccgcacgat ctgcgattct tctctgaggg cgatctgtgg ttcgcaaatc gattctcccg
     1261 ctcacctggg atgcgccgcg ggagtttcgc gaacgtctgg gcgtccggtg catgcctcgc
     1321 cgcacggtct tgggactgag gcggattcct ccccgctgcc acaacagcag cgccaaggca
     1381 gcacacgacc agcgggagcg gaagaagccg aagtcctgcg gggtggagtt cacgagccag
     1441 cgcaccacca gcagtcagga agccacgacc acggttcgcg cctctccgcc cagcacctgg
     1501 gccacgccgc cggaggagaa gtctcgcttc acgtccgcga tcagcgtggg actgacgaag
     1561 gcgcgccagc gtggaggtat ttaggttata ggaaggcctt cgcttttcgt cttttcggca
     1621 aactggtcgc tcagtcgcct cgtcattggc ccaggctggc cagcgccgac gatgcgtccg
     1681 tcgattttcg ttacggcggc cagtccgccc atcgtgcccg tgcagaaaac ctcgtcggcc
     1741 cggtagaact ccgtcagcga gaggtcgcgg acttcgtgtg gaataccctg ctcgcggcaa
     1801 atctccagga cgacggcccg cgtaattcct tccggacatg cgaccgtgcg cggtgtgtga
     1861 acttcaccgc ggtagaccag gaacaggtgc gtggcgttcg tctctgccac aaagccgcgc
     1921 gtatcgagca ttactgcgtc gtccgcgccg gccacattcg cctcaatttt ggccaggatc
     1981 gattggatca ggttgttgtg gtggatcttt gggtccaggc aatcaggcgg ggaccgccgc
     2041 acgctgctgg tgaccagcga caagccctgc tggtcataaa ccggtggctt gtgctcggcc
     2101 aacacaatta gcgttggccc ggccgtattc agccgcggat ccatgccgct ggtgattttg
     2161 acgccccggg taagcgtaag ccgaatgtgg acgtcatcgg tcatctggtt ggcggccaga
     2221 gtgcggcgaa cctgttcgat gatgtcctta tgtgtcggaa tctgggtgaa cgccagcgcc
     2281 aaggccgaac ttcgcagacg gtcgagatgt tcctttagcc ggaagattct tccctggtac
     2341 aggcgcagcc cttcccaaac tgcatcgccc ccttgcacga cggagtcgaa cgggctcacc
     2401 cccgctcggt cacggtgaat aagctcgcca ttgatgttga cgagcaggtt gcggttcgtc
     2461 tcattgaacg tttgaagcat ggttcccgtg attgtatcgc gcagcagcac gatgcgacgc
     2521 gacgtgaaat gcgtcgcacg atcacatgac cacaggatct tgccgcgaag ttacatcacc
     2581 gcgaaataat tctcgactgc caggtcgaag tattcgggcg agagcttcgg ggtctctttg
     2641 cggaacaggc agtagttctt cacctgcatc aagagatcgc ggggctggca gcagcggaga
     2701 ggacggttgt tcttcttgta gaaatgttga atcagatggt tgatcgcgct ctcgtcgtac
     2761 tcaaaaccga ggcggccgca catgagcttg aataagtctt tgaattcttc gatggtcggg
     2821 tcgacgattt cgatcttata tggaatacgg cgaagaaacg cctcatcgac caggtccttg
     2881 ggctccagat tggtcgagaa aatgatgagc tggtcgaagg ggacgtggat ttttttgccg
     2941 ttggggaggt tcaggaagtc gtatcgcttt tcgagcggga cgatccagcg gttgaggagc
     3001 tggtcgatgc tcattttctg gcggccgaag tcgtcgataa ccagcgttcc gcagttgctc
     3061 ttgagctgca gcggagcttc gctgatgccg gtcgagcggt taagcgtgac ttccaggttg
     3121 tccatctgca attcgccgcc cacgacgatc gtcggccggc ggatgcggac ccagcggcga
     3181 tcgatgcgcg attggtccaa gagaccgcta tcctgcggaa gcggggcttc ttcgtgattc
     3241 accgggtcga acaggcggat gatttcgcca tcgatgccga tcgcccgtgg aatccagaca
     3301 aactggccga acgcccgagt aacgcgctcg gcaatcgtcg tcttgccgtt gccggccgag
     3361 ccgtagagga acaacccgcg gccggagttg atcgccgggc ccaggcgatg cagcattcgc
     3421 tggttgacga ggatgtcgct gaaggcgttc ttcaggtcct gttcgctggg atgctgtttt
     3481 tcgagcgatt gagccttgac gcaacccacg tagtcggcaa gcgagacggg tgcggctccg
     3541 aagtaggtgc aatgctccat catccggcga gcccgctccc gaccgagatc agtgagctga
     3601 tagacgtaat cgttcatcgg cgccgagccg cgatagacca gaagctggtc gaatttcatc
     3661 tgccgcaaca gcggatcgag caggataaac gggaggcgga tctggtcagc aacctcgcgg
     3721 ccagtgcaat cgccgcgaga aagcaagtac ttgaggatca gcgactcgac ttcgctgtcg
     3781 gaaaggccgg catcgcggat ggtggccggt tcgatgggga tgaattcatc aggagcgggc
     3841 gcggcggcct tggctgtagc ggctggcgcg gcggaagccg cctggactgg ggcggccggg
     3901 gcggcagaat tttgcgcctg tggcggcttg ttgggatctc ctgtggccag gtcattgatc
     3961 cgccgcaaca tctcgttgag gcgggtgtcg gcctcgcgcg caccttcgtc ggacggatcg
     4021 tggtcgggct tgcgggccgc cagagcggac caaacatccc cccccttgcc gttttccttg
     4081 agcagttcca gcaggccgga atcgttgcct ttggccatga gcgcgctcgc aagctagttg
     4141 gttggccgtc ccccgtggac agcatccccc cgcaaatcta ggtcgccggt cggtgggggt
     4201 caaagaacga aaccgctgcc ggccggcggg tcgaatcgcg ccgaatgtga gactgacgac
     4261 gaatgaatcg gctgcgcctg ccgactccgg cgcagcgaat aatcgggagt tggcgaagtg
     4321 aaagctggaa aagcaattct caatctaaga tttaagtggt gggaacggtt cgagcgattt
     4381 gcgcgcccgg cgtcgccgcg cagccgaggc ccaactcttg tgcctggggc gattgtgtca
     4441 gcatacgtgg aatcaggatc tgccgaccgg tccgcgacga gcgatgaccg acgcgcagcg
     4501 ctggagcgcc tgttcctgcg aatcggcgaa gtggcgacgc tgccgacggc tgcacagaag
     4561 atcctgacgc tgacggagaa ccccgacgcc aacgtggacg gactgcgcga cgcgattcag
     4621 ggcgacccgg tgctcgtcgc gcgcctcttg cggcggctca attcctcgta ctactcgctg
     4681 agccagaaag tcaccgacct caagacagcc gtgacgctcc taggtacgcg cgagatccgc
     4741 aacttggcgc ttacggtgtt tgtttcgcgt ttctacgagg caaagggggc ccatggcaca
     4801 tacaagcgcg aagacctgtg ggcgcatagc gtcggggtgg gggcagcagc ccgacttgtt
     4861 gcccgcgtct gcgggcgggg cgctccagaa gaagctttca tcgccggttt gttgcacgat
     4921 atcggcctga ttctgctcga tcaaagcctg cggcgacact ttcatcgcgt catcgaggcc
     4981 atcgacccgg ctacgcccac gcacattgtc gaaaaccgga tcctgacgtt cgaccacgcg
     5041 atcctcggcg gctttgtggc ccgcaaatgg cggattcccg agcccgttac ggctgccatc
     5101 acgtttcacc atcagccctg gtgctacagc gggccgcaca acgaccttgt ct
//
LOCUS       DASUGB010001140         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_961449, whole genome shotgun sequence.
ACCESSION   DASUGB010001140 DASUGB010000000
VERSION     DASUGB010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961449"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="gcvPA"
                     /locus_tag="VFV87_21680"
     CDS             complement(<1..995)
                     /gene="gcvPA"
                     /locus_tag="VFV87_21680"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5106451.1"
                     /translation="MPYFYNTPGDIAAMLQAIGIGSLDELFASVPAELRLQRPLDIPP
                     ALSEIELAQHMQSLAAKNSHIGQKVCFLGGGAYDHFVPAVVDAVAGRGEFYTSYTPYQ
                     PEVSQGNLQAMFEYQTLICQLTGMDVSNASLYDGGSAAAEGVLLAMSVTSRHNKVVVA
                     ESVHPHYREILRTYFASIGAELVTVPAPSGVVMPADIAAAVDGETACVLLQHPNFFGC
                     LEDVAAIAKIAHDAGALLVQAFDPISLGLLKRPGDLGADVAVAEGQSLGTPLLYGGPY
                     LGIMACKEQFVRRLPGRIAGQTVDRRGRRCWVLTLQTREQHIRREKATSNICTNQG"
     gene            complement(1041..1436)
                     /gene="gcvH"
                     /locus_tag="VFV87_21685"
     CDS             complement(1041..1436)
                     /gene="gcvH"
                     /locus_tag="VFV87_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5106452.1"
                     /translation="MNPKDLLYAETHEWTRVADEGGRKVATIGITAFAIQQLTDLVHM
                     VLPKVGTQVKAGSPFGEVESVKAVSDLYSPVDGEVIAAHGELPNKLETLGSDPYGEGW
                     MIKVAISDQSSLKKLMDAAAYEKQCAAEG"
     gene            complement(1442..2602)
                     /gene="gcvT"
                     /locus_tag="VFV87_21690"
     CDS             complement(1442..2602)
                     /gene="gcvT"
                     /locus_tag="VFV87_21690"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5106453.1"
                     /translation="MSGSVRKTPLFDWHAAHGGRLVDFAGWSMPVQYGSIVEEHLATR
                     RACGLFDVSHMGRLRLDGAGVAAFLDRLLTRKVSGMGPGRIRYSLVCNESGGILDDVL
                     IYHLQEHGGGLYHLLVVNASNREKIVSWLQSHLPAANDVQLADRTTETAMIAVQGPAA
                     LKIVEPLVGADLGGLGYYTGLETTICGHHGIASRTGYTGEDGCELIVPVAAAVEVWEK
                     VLAHGKDGGAVAAGLGARDTLRLEAAMPLYGHELSETINPIQAGLNFAVNLEGREFIG
                     RSALIAARNDQTAPVRVGLVLAGRRVPREHYPVLGSPRAPREDGISRSEMPTMAVGEV
                     TSGTFSPTLQKPIAMAYVQPDVAAVGTPLAVNIRGTIEPATVVKLPFYTRAS"
     gene            2771..>4070
                     /locus_tag="VFV87_21695"
     CDS             2771..>4070
                     /locus_tag="VFV87_21695"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5106454.1"
                     /translation="MSQSPPQPDPAKVEVLPRILGPFDAVMLVVGSIIGSGIFLKVSN
                     IDNLVPSFGTIMLVWIVGGIATLCGSLSLAELAAMLPHAGGPYVYLREAYGRVTAFLW
                     GWAEFSIIRTGSLGSLACGTVIYFNKMLDSAEKTGLLPEFLADYVPLTHWGQCVVTVL
                     AVLVLTWINAIGLRAAARTQNITTVIKVGFLLVLMLSPLAFGSWSVSNLQPIAPPEFN
                     LNLFKAFGLAMVAVFWPYDGWINMGPVAEEVREPQRNVPLGLGLGIIVVMLVYCGANI
                     GYHLCLPMQDIMASETVAADVFAKVLGRYGVPLAAAGVMISMFGALNSNLLAGPRIYF
                     AMARDRLFPRAIGHVHERFKTPLNAVLAQSIWAITQILIVFSIVEQPRDAFNTLTDFV
                     VLGGTIFYALVVAAVIVLRFKLPKLERPYRVWLYPITPLLY"
BASE COUNT          679 a         1344 c         1260 g          787 t
ORIGIN      
        1 ccctggttcg tgcagatgtt gctcgtcgcc ttctcgcggc ggatgtgctg ctcgcgggtc
       61 tgcagcgtca gcacccagca ccgccgcccg cggcgatcga ccgtctgccc ggcaattcgc
      121 cccggcagcc ggcggacgaa ctgttccttg caggccatga tgcccaggta aggcccgcca
      181 tacagcagcg gcgtgccaag cgactgcccc tcggcgacgg caacatccgc gccgagatcg
      241 cccggccgtt taagcaggcc caggctgatc ggatcgaacg cctggacaag cagcgcgccg
      301 gcgtcatgcg cgatcttggc gatcgccgcg acatcctcca ggcagccgaa gaagttcggg
      361 tgctgcagca agacgcaagc ggtctcgcca tcgacggccg ccgcgatatc cgccggcatt
      421 acgacgccgg atggcgcggg aaccgtcacc agctccgcac cgatgctggc gaaatacgtc
      481 cgcaggatct cgcggtagtg cggatgcacg ctctcggcga cgacgacctt gttgtgccgc
      541 gaagtcacgc tcatcgccag gagcacgcct tccgccgcgg cgctgccgcc gtcgtagagg
      601 ctggcgttgc tgacatccat cccggtgagt tggcagatca gcgtctgata ctcgaacatc
      661 gcctgcagat tgccctggct tacctccggc tgatagggcg tgtacgacgt gtagaactcg
      721 ccgcggccgg caaccgcatc gaccacagcc ggcacgaagt gatcgtaagc tccgcccccc
      781 aggaagcaga ctttctggcc gatgtgggag ttcttcgccg ccagcgattg catgtgctgg
      841 gccagctcga tttccgacag ggcagggggg atgtccagag gccgctgcag tcgcagctca
      901 gccggcacgc tggcaaacaa ctcgtccagc gagccgatgc cgatggcctg cagcatcgcg
      961 gcgatgtcgc caggcgtgtt gtagaagtaa ggcatatacg aatgcggagt gcggaattcg
     1021 gaatgcggag tgatggatta ttaaccttcc gccgcacatt gcttttcata ggccgcggca
     1081 tccatcagct tcttcagcga cgattggtcg ctaatcgcga ccttgatcat ccagccctcg
     1141 ccatagggat cgctgccgag cgtttcgagc ttgttgggga gttcgccgtg cgcggcgatc
     1201 acttcgccat cgaccgggct atacaggtcg ctcactgcct tgaccgactc gacttcgccg
     1261 aatggcgagc cggctttgac ttgtgtgccg accttcggca gcaccatgtg gaccaggtcg
     1321 gtgagctgct ggatggcaaa cgcggtgatc ccgatggtag cgaccttgcg gcctccttca
     1381 tcggccacgc gggtccattc gtgtgtttcg gcatagagca ggtctttagg gttcatatcg
     1441 ctcagctcgc gcgggtataa aatggaagtt tgacgaccgt cgccggctca attgtgccac
     1501 ggatattgac ggcgagtggc gtgccgaccg ccgccacgtc cggttgcacg tacgccatcg
     1561 cgatcggctt ttgcagcgtg ggcgagaacg tgccgctggt gacttctccg acggccatgg
     1621 taggcatctc gctccgcgag atgccatcct cgcgcggagc gcgaggacta ccaaggaccg
     1681 gatagtgctc acgcggcacg cgccggccgg cgagcacgag accgacccgc acgggcgctg
     1741 tctgatcatt cctggcagcg atcaaggccg atcggccgat aaactctcgc ccttcgagat
     1801 tcactgcgaa gttcagcccc gcctgaatcg ggttgatcgt ctcgctcagc tcgtggccat
     1861 acagcggcat cgcggcttcc aggcgcagcg tgtcgcgggc gccgaggccc gcggccaccg
     1921 cgccgccatc tttgccgtgg gcgagcactt tctcccagac ctccacggcc gccgcgaccg
     1981 gcacgatcaa ttcgcagccg tcttcgcccg tgtaacccgt gcggctggcg atgccatgat
     2041 gaccgcagat cgtcgtttcc aggccggtgt aatagcccag gccgcccaga tcggctccga
     2101 cgagcggctc gacgatcttg agtgccgccg gcccttgcac ggcgatcatt gccgtctcgg
     2161 tcgtccggtc ggcgagttgc acgtcgttgg cggccggcaa gtgcgactgc agccagctca
     2221 cgatcttctc gcgattgctg gcgttcacga cgagcaggtg atagaggccg ccgccatgct
     2281 cttgcaggtg atagatgagc acgtcgtcaa ggatgccgcc gctctcgttg cagacgagtg
     2341 agtagcgaat ccgccccggc cccatgccgg agactttccg cgtcagcagc cggtcgagaa
     2401 atgccgcgac gccggctccg tccagtcgca ggcggcccat gtgcgagacg tcgaacaggc
     2461 cgcaggcccg ccgcgtcgcc agatgctctt ccacgatcga gccgtactgc accggcatcg
     2521 accagccggc aaaatcgacc agccggccgc catgcgcggc atgccaatca aacagcggcg
     2581 tcttgcggac ggagccggac atcagcggtc aacttgtgaa ggaacggggg gggactggtg
     2641 cattgtatcg gcgtgggggg gcgaagctag gcgggagtcc aaagtccaac gtccaaggtc
     2701 caaagttgcc aatctcgacc ttggactttg gactttggac cttggactgt ccttccgcta
     2761 tgctgcctcc atgtcgcagt cgcctcccca acccgatccc gcgaaagtcg aagtcctgcc
     2821 gcggattctt ggccccttcg acgccgtgat gctcgtcgtc ggctcgatca tcggctcggg
     2881 catctttctg aaggtcagca acatcgacaa cctggtgccc tcgttcggca cgatcatgct
     2941 cgtctggatc gtcggcggca tcgccacgct gtgcggctcg ctatcgctgg cggagctagc
     3001 cgcgatgctg ccgcacgcgg gcggaccgta cgtctatctg cgcgaagctt atggccgcgt
     3061 gacggcgttt ctgtggggct gggccgagtt ctcgatcatt cgcaccggct cgctcggcag
     3121 cctggcctgc ggcacggtca tctacttcaa caagatgctc gattcggccg agaaaacggg
     3181 cctgctgccg gagtttctcg ccgactacgt cccgctgacg cactgggggc aatgcgtcgt
     3241 caccgtgctg gcggtgctcg tgctgacgtg gatcaacgcc atcggactgc gggcggcggc
     3301 ccggacgcaa aacatcacga cggtcatcaa ggtcggcttt ctactcgtgc tgatgctcag
     3361 cccgctggct ttcggctcgt ggagcgtctc caacctgcag ccgatcgcgc cgcccgaatt
     3421 caatctcaac ctgttcaaag ccttcggcct ggcgatggtt gccgtcttct ggccttacga
     3481 cggctggatc aatatgggtc cggtcgccga agaagtccgc gagccgcagc ggaacgtgcc
     3541 gctcggcctg ggtctgggca ttatcgtcgt aatgctcgtc tattgcgggg cgaatatcgg
     3601 ctatcacctc tgcttaccga tgcaagacat tatggcctcg gaaacggtgg ccgccgacgt
     3661 gttcgcgaag gtccttggcc gttacggcgt gcctctcgcc gcggcaggcg tgatgatttc
     3721 gatgttcggc gcgctgaact ccaacctgct cgccggcccg cgaatctatt tcgccatggc
     3781 tcgcgaccgc ctcttcccgc gggcgattgg ccacgtccat gagcggttca agacgccgct
     3841 caacgcggtc ctcgctcagt cgatctgggc gatcacgcaa atcctcatcg tgttctcaat
     3901 cgtcgagcag ccccgcgatg ccttcaacac gctcaccgac ttcgtcgtcc tcggcggcac
     3961 gatcttctat gccctcgtcg tcgccgcggt catcgtcctg cggttcaagc tgccgaagct
     4021 ggaacggccc taccgcgtct ggctttatcc cataacgccg ctcctctact
//
LOCUS       DASUGB010001141         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2072607, whole genome shotgun sequence.
ACCESSION   DASUGB010001141 DASUGB010000000
VERSION     DASUGB010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072607"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFV87_21700"
     CDS             <1..328
                     /locus_tag="VFV87_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106455.1"
                     /translation="KVVAARNSAGFNPARREDLRLFAAVLDGDAIARGFRNKDIRAAL
                     YGNEPSPSPRHSAAVGRWLKRLHVRQLVAKIPHTRRWRITTAGRQLLSRAVQLYQQTW
                     PELLAA"
     gene            complement(363..1856)
                     /locus_tag="VFV87_21705"
     CDS             complement(363..1856)
                     /locus_tag="VFV87_21705"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX5106456.1"
                     /translation="MSTDASLPTNVEALQRELLHTQSVLAETAVTCEQLRLQNEKLQA
                     ELDLFQRYLYGRRSERFVEDPGQGRLFEQPAEGGPPTPQLPEAAEEELTYRRRRKGHG
                     WSELPPHLPREKVLLDLPAAERTCGCCGERMQQIGEDRTERVDYRPARIVVKVFVTPK
                     YACPKKHGGVKQAETPPGPVPGGSFDFGLVAQVVTSKICDHLPLYRQQDVLARAGIEL
                     SRSTLCEIMASAAFLLEPLAALLKQRLLASDWLGADDTPVRLLDRAHPEGVRLARFWL
                     YRGPETSPYNAFDFHESRSRDGPRKFLGPYQGWVKVDAYGVDGGVYLGSARMRASCCL
                     AHARRKFDEAKSSHPRLAAEALGFFQQLYDLEDRARELSAEARQALRQAEAAPLLEKL
                     RTWADEQAAQALPKLKFGEALGYLRNQWEPLTNYVQDGRLPIDNNATERDLRALTIGR
                     KNWLFIGSQEAGPRAAILYTVVASAARHDLDVWAYPSGPKSALVSYQ"
     gene            complement(1935..2282)
                     /gene="tnpB"
                     /locus_tag="VFV87_21710"
     CDS             complement(1935..2282)
                     /gene="tnpB"
                     /locus_tag="VFV87_21710"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX5106457.1"
                     /translation="MLNVPAKVRIFFCRSAIDMRKGFDGLHGVVLEVLRQDPLSGDLF
                     VFLNKRRDRVKLLIWEGDGMLIVYKRLERGTFALPAGTGDSLVLTSAQLALLLGGIDL
                     KQTRPRARYQRAG"
     gene            complement(2276..2626)
                     /locus_tag="VFV87_21715"
     CDS             complement(2276..2626)
                     /locus_tag="VFV87_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106458.1"
                     /translation="MGRVASEALSSVWERRIARQGRSRLSIAEFCSQEGVSTASFYVW
                     RRRLRGADAAAPQSPLFVPVELPTPGIAAGGVRVELPGGVILSLPADASVELVTAAIR
                     AVMSIAAAGERPSC"
BASE COUNT          510 a          998 c          848 g          476 t
ORIGIN      
        1 caaagtcgtg gccgcccgca actcggccgg cttcaacccg gcccgccgcg aagacctccg
       61 cctgttcgcg gccgttctgg acggtgatgc cattgcccgc ggcttccgca acaaagacat
      121 tcgcgctgcg ctgtacggca acgaaccatc gccttcaccc cgtcacagtg ccgccgtcgg
      181 ccggtggctc aaacgacttc acgttcgaca gctcgtcgcc aaaatccccc atacccggcg
      241 ctggcgcatc accaccgccg gccgccagct cctcagccgc gccgtccaac tctatcagca
      301 aacctggccc gaactcctcg ccgcttaatc ccgcgaaact tctacgcagc ttgcatggaa
      361 gatcactggt aagaaactaa cgcagacttt gggcccgacg gataggccca cacgtccagg
      421 tcatgccggg cggcgctagc gacgacggtg tagaggattg cggcgcgcgg gccggcctct
      481 tggcttccta tgaataacca gttttttctg ccgatcgtga gcgcccgcag gtcgcgctcc
      541 gtcgcgttgt tgtcgatcgg cagccggccg tcctgaacgt agttggtcag cggctcccac
      601 tgattgcgta aataacccaa ggcttcgccg aacttcagct tgggcagggc ctgtgccgct
      661 tgctcgtcgg cccaggtgcg gagtttctcc aagagcggcg ctgcttccgc ctgccgcagc
      721 gcctgccgcg cctcggccga aagttcccgc gctcgatcct cgaggtcgta aagttgttga
      781 aagaaaccca aagcttccgc cgccagtcgc ggatgagacg acttggcctc gtcaaacttg
      841 cgccgcgcat gggccaggca gcagctcgcg cgcattcgcg cgctgcccag atacacgccg
      901 ccgtccactc catatgcatc gaccttcacc cagccctggt aagggcccag gaacttccgc
      961 gggccatccc ggctgcggct ctcgtggaag tcgaatgcgt tgtagggcga ggtctccggc
     1021 ccgcgataca gccagaagcg agccagccgc acaccttcgg gatgggctcg gtcgagcagt
     1081 cgcaccgggg tgtcgtcggc gcccagccag tcgctggcca agagccgctg tttcaagagc
     1141 gctgccaacg gttccagcaa gaacgccgcg ctggccatga tctcacagag cgtcgagcgg
     1201 ctgagctcga tgcccgcccg ggccaggacg tcttgctggc gatagagcgg caagtggtca
     1261 cagatcttac tggtgaccac ctgggccacc aggccgaaat cgaaactccc gccgggcacg
     1321 gggccaggtg gagtttcggc ctgtttcacg ccgccgtgct tcttcgggca ggcgtacttg
     1381 ggcgtcacga agaccttcac cacgatccgg gccggccgat aatcgactcg ttcggtgcga
     1441 tcttcgccga tctgctgcat ccgttcgccg cagcagccgc aggtccgctc cgcggcgggc
     1501 aggtccaaca ggactttttc ccgcggcagg tgcgggggca gttcactcca gccgtgaccc
     1561 tttcggcgac ggcgataggt gagctcttct tcggcggctt ccggtagctg cggagtcggc
     1621 ggaccgccct cggccggctg ctcgaacagg cggccttggc cggggtcttc aacgaagcgt
     1681 tcgctgcggc ggccgtagag ataacgctgg aaaagatcga gctccgcttg cagcttctcg
     1741 ttctggaggc ggagctgttc gcaggtgaca gccgtctcgg ccagcacgct ttgcgtgtgc
     1801 aatagctcgc gctgcagagc ttccacattc gtaggcaagg aggcgtccgt gctcatggaa
     1861 gcctaaacgc acgagccggc cgtttggttc cgcagaccgt cgagtttttt gcacaaccgt
     1921 tctcgggccg ctgctcagcc cgcgcgttga taccgcgctc gaggccgggt ttgcttgaga
     1981 tcgatgccgc ccaggagcag tgcgagctgc gccgacgtga gcacgagcga gtcgccagtg
     2041 ccggcgggta gcgcgaacgt cccacgttcc aatcgcttgt aaacaatgag catgccgtct
     2101 ccctcccaga tcagtagctt cacgcggtcg cggcgcttgt tgagaaacac aaacagatcg
     2161 ccggacagcg gatcctggcg cagcacttcc agcaccacgc cgtgaagccc gtcgaagccc
     2221 ttccgcatgt cgatcgcgga ccggcagaag aaaatgcgga ccttggcggg cacgttcagc
     2281 acgacggccg ctcccccgct gcagcgatcg acatgacggc gcggatcgcc gcggtcacca
     2341 gttccacaga cgcgtctgcc ggcaggctca gaatgactcc gccaggcagc tccacgcgca
     2401 caccgcccgc tgcgattccc ggcgtcggta attcgactgg cacgaacaga ggggactgcg
     2461 gcgccgcagc atccgctccc cgcaaccgcc gccgccagac ataaaacgaa gctgtggaga
     2521 ccccttcctg gctgcagaac tcggcgatcg acaaccggct ccgcccctgc cgcgcaatcc
     2581 gccgctccca gaccgaagac aacgcctcgc tcgccacgcg ccccatgacc catccctcac
     2641 cgcgagaaga aactgaacac cccgcagctt aaagcacgcg ccaaggatgg atgggccgga
     2701 cggttacgca gcatccgctc cccgcaaccg ccgccgccag acataaaacg aagctgtgga
     2761 gaccccttcc tggctgcaga actcggcgat cgacaaccgg ctccgcccct gccgcgcaat
     2821 ccgccgctcc ca
//
LOCUS       DASUGB010001142         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_405276, whole genome shotgun sequence.
ACCESSION   DASUGB010001142 DASUGB010000000
VERSION     DASUGB010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405276"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VFV87_21720"
     CDS             complement(<1..1170)
                     /locus_tag="VFV87_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5106459.1"
                     /translation="MTTILGISAFYHDSAAALVRDGRIVAAAQEERFSRVKHDASFPS
                     QAIAYCLAEAGLAPEQLDYVAFYDKPLAKFERLLETSLAYAPLGFGSFREAMPVWLRE
                     KLHLRGRINRELRRSRHQPLVFHDHHESHAASAFFPSPFDEAAILTLDGVGEWSTTTL
                     GIGRRNEIELTQHIAFPHSLGLLYSAFTAYCGFEVNDGEYKLMGLAPYGQPVYADAIR
                     RHLIDLKADGSYRLNMEYFAYGYRQAMTNRRFHRLFGGPPRQPEALLKQRHADLAASI
                     QAVIEEAILRIAAELHRRTGMDNLVMAGGVALNCVANSRLLREGPFRSIWIQPAAGDA
                     GGALGAALFVWHQLLNQPRTADPSDSQGGSLLGPQFTSAAIRTLLDGAGARYRRLA"
     gene            complement(1167..2678)
                     /locus_tag="VFV87_21725"
     CDS             complement(1167..2678)
                     /locus_tag="VFV87_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5106460.1"
                     /translation="MAATIQSEAQDKQRAEPPRRRGAMWKAILFRGAAVLLGLSPLLA
                     CELTLRALRLGKPTDFNDPFVGFSDVHPLFVLNQANGRYEIPKSRQTHFQPESFAAKK
                     PAGEFRIFVLGGSTVQGRPWSIETSFTTWLELNLNAADRARTYEVVNCGGVSYATYRL
                     VPILQEVLKYEPDLIIFCEGHNEFLEDRSYGSIKRASPVLAWPQRQVSRLRTYNVLRE
                     GLLQLTARPDSAANDKRAILGPEADARLDWKDGIAKYHRDAKWQADVIAHFEISLQRI
                     AAIADGAGVPLIFVSPVSNLQWAPFKSQHREGITPAERNEFESLLQRASELDATDTPA
                     AVKLLRQALTIDDQHAGVHYEIGLRLLEMGMSLEARPELVEAKDLDVCPLRMLEPMKS
                     IMHDVAAETGTPLVDADALLAAQSRSGFPDRQWLVDHVHPTMEGHQLIAGAIAAKMAE
                     LGFVQPEAGWEAKRDQAYREHLASLSVVYFQRGKDRLRSEQGWAHGKVQRVKP"
     gene            complement(2759..3703)
                     /locus_tag="VFV87_21730"
     CDS             complement(2759..3703)
                     /locus_tag="VFV87_21730"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX5106461.1"
                     /translation="MPCPSTRFRLILRAVCGIALLAIAVPADAQEGPVLERPLVRLPP
                     IEEELPTPAAQVAAEAEPASPSDKPPADPSAADEALVKVIDHEPGWHVWQPDFWDPWE
                     GSVELGLSGTEGNSQTFNVRFGLNAKHKTERMIQTLQITSIQKSADGVTTANTALADG
                     RIEWPMPGSKWSYHIHSLLERDQFKAFDYRVSADTGVGYEFAKNDVTTLLGRAGLSAS
                     REIGGPDNEVKPELALGSEYKHQFNATHSVSAKVDYFPNVTDFNDFRLNSQASWEIAL
                     SSAWGLSLKLSVIDRYDSTPQGKRPNDLDYSTLLLWQF"
     gene            complement(3784..>4128)
                     /locus_tag="VFV87_21735"
     CDS             complement(3784..>4128)
                     /locus_tag="VFV87_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HEX5106462.1"
                     /translation="DLPDVSWFSALGTAVDWHGDDSTLTALLKAVEPEHDPAGAARDV
                     LILANATSEPREFILPPLAKGTRWRLFLDTAAATPYDVYPEMDGPPPPRSRRLTLSYR
                     SLCVFVAEKGRK"
BASE COUNT          712 a         1349 c         1305 g          762 t
ORIGIN      
        1 ggccagccgg cgatacctcg cgcccgcgcc gtcgagaagt gtccggattg cggccgaagt
       61 aaactgcggg ccaagcaggc tgccgccttg cgaatcgctc ggatcggctg tgcgcggctg
      121 attgagaagc tgatgccaga cgaagagcgc cgcgccgagc gcgccaccgg catcgccggc
      181 ggcgggctgg atccaaatac tccgaaaagg tccttcccgc agcagccggc tgttggcgac
      241 gcagttgagc gcgacgccgc cggccatgac gaggttgtcc atgccggtgc ggcggtgcag
      301 ctccgccgcg attcgcagaa tggcctcctc gatcaccgct tgaatgctcg ccgccaggtc
      361 agcgtgccgc tgcttgagta acgcttccgg ctgccgcggc gggccgccga acagccggtg
      421 gaaccgccga ttcgtcatcg cctgccggta gccgtaggcg aaatattcca tgttcaaccg
      481 atagctgcca tcggctttga gatcgatcag gtgccggcgg atcgcgtcgg catagaccgg
      541 ctggccatag ggcgccagcc ccatcagttt gtattcgccg tcgttcacct cgaagccgca
      601 ataggcggtg aatgcggaat agagcagccc cagcgagtgc ggaaaggcaa tgtgctgcgt
      661 cagctcgatc tcgttgcggc ggccgatccc cagcgtcgtc gtgctccact cgccgacacc
      721 gtcgagcgtc aggatcgcgg cctcgtcgaa tgggctggga aagaacgcgc tggcggcatg
      781 gctctcgtga tggtcgtgga agacaagcgg ctggtggcgc gagcggcgca gctcgcggtt
      841 gatgcgaccg cggaggtgga gcttctccct cagccacacc ggcatcgcct cgcgaaagct
      901 gccaaagccg agcggagcat aggcgaggct tgtctccagc agccgttcga acttagccag
      961 cggtttgtcg tagaaggcga cgtagtcgag ctgctcgggc gcaaggccgg cttcagccag
     1021 gcaataggca atcgcttgtg aaggaaaact cgcgtcgtgc ttgacccggc tgaagcgctc
     1081 ttcctgggcg gccgcgacaa tcctgccgtc gcgaaccagc gcggccgcgc tgtcgtgata
     1141 gaaagccgag atgccgagga tcgtggtcat ggcttgacgc gctggacttt gccgtgggcc
     1201 cagccttgct cggagcgcag gcggtccttg ccgcgctgga aatagacgac cgagaggctg
     1261 gcgaggtgct cgcgataagc ctggtcgcgc ttggcttccc aacccgcttc aggttgcaca
     1321 aaccccagct ctgccatctt ggcggcaatg gcgccagcaa tgagctggtg gccctccatc
     1381 gtcggatgaa cgtgatcgac cagccactgc cggtcgggaa agccgctgcg gctttgcgct
     1441 gcaaggaggg cgtcggcatc gaccagcggc gtgccggttt cggcggcgac gtcgtgcatg
     1501 atcgacttca tcggctccag catccgcagc gggcacacgt cgaggtcttt ggcctcgacc
     1561 aattctggcc gcgcctcgag cgacattccc atttccaaca aacgaaggcc aatctcgtaa
     1621 tgaacgccgg cgtgctggtc gtcgatcgtc aaagcttgcc gcagcaactt tacggccgcc
     1681 ggcgtatcgg tggcatcgag ctcactcgcc cgctgcagga gcgattcgaa ttcgtttcgc
     1741 tcggcaggcg tgattccctc gcgatgctgg gacttgaacg gcgcccactg caggttcgag
     1801 accgggctga cgaaaatcag cggcaccccg gccccatcgg caatcgccgc gatccgctgc
     1861 aggctgatct caaagtgggc gatgacatcc gcctgccact tcgcatcgcg gtggtacttc
     1921 gcgataccgt ccttccagtc gaggcgcgca tcggcctcgg ggccgaggat cgcgcgtttg
     1981 tcgttcgctg cggaatcggg ccttgcagtc agctgcagca gcccctcgcg gagcacgttg
     2041 tatgtccgca gccgcgacac ctgccgctgc ggccaggcaa gcaccggcga cgcacgtttg
     2101 atcgagccgt agctgcggtc ttccaagaac tcgttgtgcc cttcgcaaaa aatgatcagg
     2161 tccggctcgt acttcagcac ttcctgcaga atcggcacca ggcgatacgt cgcgtaggaa
     2221 acgccgccgc agttgacgac ttcgtacgtt cgcgcccgat ctgccgcatt gaggttcaac
     2281 tccaaccacg tcgtgaagct cgtctcgatc gaccacggac ggccctgcac cgtcgatccg
     2341 cccagaacga agatgcggaa ctcgccggcg ggcttcttgg ccgcaaacga ctccggctga
     2401 aaatgcgtct gccgcgattt gggaatctcg tatcgcccgt tcgcttgatt cagcacgaac
     2461 agcggatgaa cgtcgctgaa cccgacgaac gggtcgttga aatcggtcgg cttgccgagc
     2521 cgcagcgcgc gaagcgtcag ctcgcacgcc aacaggggcg acagcccgag cagcaccgcc
     2581 gcgccgcgga atagtatggc cttccacatt gcaccgcggc ggcgaggcgg ctcagcgcgc
     2641 tgcttgtctt gtgcttcgga ctgaatcgtc gccgccatga gttcatcgta tccgcagcct
     2701 ccagccgatc ataggtttcg ccaagtccaa aggcggtgac gagtcatcgc actccaaatt
     2761 aaaactgcca caagagcagc gtcgagtaat ccaggtcgtt ggggcgcttg ccttgtggcg
     2821 tgctgtcgta gcggtcgatc acgctcagct tcaggctcag cccccaggcg ctggaaaggg
     2881 cgatctccca cgaggcctgg ctgttgagac gaaaatcgtt gaagtcggtc acgttcggaa
     2941 agtaatcgac cttcgcgctc accgagtgcg tggcgttgaa ttggtgcttg tactcgctgc
     3001 ccagcgccag ttccggcttc acttcgttgt cggggccgcc gatctcgcgc gaggcggaga
     3061 gaccggcccg gcccaagagc gtcgttacat cgttcttggc aaactcgtat cccacgcctg
     3121 tgtcggcgct cacgcgatag tcgaatgcct tgaattggtc gcgttcgagc aagctgtgaa
     3181 tgtggtaact ccatttcgag cccggcatgg gccactcgat ccgcccgtcg gccaaggccg
     3241 tgttggcggt cgtcacgccg tcggctgatt tctggattga cgtgatttgc agcgtctgaa
     3301 tcatccgctc ggtcttgtgc ttcgcgttca ggccaaaccg gacgttgaac gtttgtgagt
     3361 tcccttccgt cccgctcaag cccagctcga cgctcccttc ccagggatcc caaaagtccg
     3421 gctgccagac gtgccagccc ggctcgtggt cgatcacctt gaccagcgct tcgtccgccg
     3481 cactcgggtc ggcgggcggc ttgtcggaag gcgaagccgg ctctgcttcc gctgctactt
     3541 gcgccgctgg tgttggcaac tcctcctcga tcggcggcag gcggaccagc ggacgctcca
     3601 gcaccggccc ttcctgcgca tctgctggca cagctattgc tagcagcgcg atgccgcaca
     3661 cggcgcgcag gatcagccgg aatcgagtgc tagggcaagg catgtcggcc ggaccttagc
     3721 aaacgccgcc gcttgcggaa aggcggccta acgtccctgt cttctaaacc ccggaccctg
     3781 tctctacttc cttcctttct ccgccacgaa cacgcacagc gagcggtacg acagcgtcag
     3841 ccgccggctg cggggcggcg gggggccgtc catctccgga tacacgtcgt acggcgtcgc
     3901 tgccgccgtg tcgagaaaca gccgccaccg cgttcccttg gcgagcgggg gcaaaatgaa
     3961 ctcccgcggc tcgctggtgg cgttggccag gatcagcacg tcccgcgccg ctcctgcggg
     4021 atcgtgctcg ggctcgacgg ccttgagcag cgccgtcagc gtcgaatcgt cgccgtgcca
     4081 atcgaccgcg gtccccagcg cgctgaacca gctcacgtcc ggcaggtc
//
LOCUS       DASUGB010001143         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1238698, whole genome shotgun sequence.
ACCESSION   DASUGB010001143 DASUGB010000000
VERSION     DASUGB010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238698"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..926)
                     /locus_tag="VFV87_21740"
     CDS             complement(117..926)
                     /locus_tag="VFV87_21740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5106463.1"
                     /translation="MNFFGTQDGAGARVWSAGKFVADKQNDEQLKARIRELESLCTDV
                     LVAGVDIGLPQHLLNQLWAAVGHGELPHAYHVDLPPAPPRPKEPSAPQNKVAPGTFNK
                     DAAQARPEPMAIPDIPLTVNPLAAKEESRSKPPQAELKPLNVKKTVCVVDDDPMMLDV
                     LSRILQRENYELLMASGGPEMMDKLSAHNGSVDLLVTDYAMPEMQGRELADHVRQRFP
                     AVKVLYQTGFSDMLFENRVELEEGAAFLEKPFTARGLREAARLVLFGSINP"
     gene            1267..2049
                     /locus_tag="VFV87_21745"
     CDS             1267..2049
                     /locus_tag="VFV87_21745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5106464.1"
                     /translation="MRTTGRRRHLSTAEQEDFAGFVNLRLIEDDYAILRKFQSRSSLW
                     TYLAAVIERLSLDFCAEKWGRWRPSAMAERLGPAAVVLERLVVRDSNPLEEAFEILRA
                     QHGVTLSEAELRRIWEQLPVRLRTSEVSEEAAAAVSGPETADVNIDDAERRASVARLQ
                     SVLQTAFERIPAQDRVLIALRFDEELSMAEIAKLLKSSIPTLHRRLDKGLKELRAALT
                     HSGFEPREVAKLIGHPTIALSPLLRTEVERFLGPVRLSKRDG"
BASE COUNT          436 a          768 c          789 g          474 t
ORIGIN      
        1 ccaggatcgg ccgcggcggt ggaaacacct gcgaggaaaa acaggaaaac aaataggcgg
       61 cgcatagacg aatcccgtga agcacgggac ccaccattat tacgcctgac agatctttac
      121 ggattgatcg atccgaacag cacgagccgc gccgcttccc tcaagccgcg cgccgtgaag
      181 ggcttctcga gaaacgcagc gccctcctcg agctcgacgc ggttttcgaa cagcatgtcg
      241 ctgaagccgg tctgatacag gaccttgacg gctgggaaac gctggcggac gtgatcggcg
      301 agttctcgtc cttgcatttc cggcatcgcg tagtcggtca ccagcaggtc gacgctgccg
      361 ttgtgggctg acagcttgtc catcatctcc ggcccgccgc tggccatcag caattcgtaa
      421 ttctcccgct gcaggatgcg cgacaagacg tccagcatca tcgggtcatc gtcgaccacg
      481 cacacggtct tcttgacgtt gagcggcttc agttcggctt gtggcggctt gctgcggctt
      541 tcttccttgg ccgccagcgg attgacggtc aacggaatat ccgggatggc catcggttca
      601 ggccgggcct gggcggcgtc cttgttgaaa gttccggggg caaccttgtt ctgcggcgcg
      661 gacggctctt tggggcgcgg cggcgccggc ggcaggtcga cgtgatacgc gtgcggcaat
      721 tcgccgtgac cgacagccgc ccagagctgg ttcagcaggt gctgaggcag gccgatgtca
      781 acgccggcga ccagcacgtc cgtacacaac gattccagtt cgcgaatgcg agctttgagc
      841 tgctcgtcgt tttgcttgtc cgccacaaat ttaccggccg accagacgcg ggcgcctgcc
      901 ccatcctggg tgccgaagaa gttcacagcc atactaccgt gcttcgccat tccgcttgac
      961 ctcttcccat ccgtacaccc cgcgcacatg aacgcgcaca tttctgcacg cgcgagccgg
     1021 atggcagggg cgctgaatcc ggccgggttc cgtggagtca cctcggacga gccggattga
     1081 aagttgcttc cacatcaata gacggccagg cgtcgtcaag ttctcatccc tgaagtctgc
     1141 gtgcaggtaa ttacaaaagt gtgtcccgcc gctgctcttg agccgataat ccggtcgagt
     1201 tcccctcatc tccgcgtgga ctaccaacgg ctgctcatcg accacctaca tctaatcgat
     1261 cagatcgtgc gtacgaccgg ccgccggcgg cacttgtcga cggccgagca ggaggacttt
     1321 gccggtttcg tcaacctgcg gctgatcgag gacgactacg cgattcttcg gaagttccag
     1381 agccgcagtt ccctgtggac gtatctcgct gccgtcatcg agcgcctgtc gctcgacttc
     1441 tgcgctgaga aatggggccg ctggcggccg tccgccatgg ccgagcgcct tgggccggcc
     1501 gcggtcgtgc tggaacgcct tgtcgttcgc gactcgaatc ccctggaaga ggccttcgag
     1561 attctgcggg cccagcacgg cgtcacgttg agcgaggccg agctgcgcag gatctgggag
     1621 caactccccg tgcgcctgcg gaccagcgaa gtcagtgaag aggccgcagc cgccgtgagt
     1681 ggccccgaaa ccgcggacgt caacatcgac gatgccgagc gccgggccag cgttgcgcgg
     1741 ctgcagtcgg tgctgcagac ggctttcgaa cggattcccg cgcaagatcg agtgttgatt
     1801 gccctgagat ttgatgaaga gctgtcaatg gctgaaatcg ccaaactgtt gaagagctca
     1861 atcccgaccc tgcacagaag gctggataaa ggcctgaagg aattgcgggc ggcgctgacg
     1921 cactcggggt tcgaaccacg ggaagtcgcc aagctcatcg gccacccgac gatcgccctc
     1981 tctcccctgt tgcggaccga ggttgagagg tttttgggac ccgtccgtct atctaagcga
     2041 gatggttgat ccacacacca acggttgccc ggaaccggag gccctggcgg catacgtcga
     2101 ccagggattg agcttggctg agcgcgcacg cgtggacgct catctcgcct cgtgcccgca
     2161 gtgcatcgcg ctgctggccg gggtcgctcg aacggtcgcg gaagtgtcag cgcacgtgcc
     2221 tggcgcaggc gtgccggcgg aggccactcc gctggtgtcg cggcggtccc tggcaggggc
     2281 gttggcggct gcggctgccg tgatcgcggt gattgccgcg ccctcacttg tgaggccgtg
     2341 ggttgatcgc gacgcgaggc tcgtgaatct tgtcgaaagt gtcggtgaac aacgatcggt
     2401 tctcggcagg ttaaccgggg ggttcccgca tgccccactg ggtgcgccat ctgctggggg
     2461 gcaagat
//
LOCUS       DASUGB010001144         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1932917, whole genome shotgun sequence.
ACCESSION   DASUGB010001144 DASUGB010000000
VERSION     DASUGB010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932917"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFV87_21750"
     CDS             complement(<1..1064)
                     /locus_tag="VFV87_21750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106465.1"
                     /translation="MTEREIFDRALAIADPAGRAAWLDEACGGDPARRDHIAGLLEAH
                     LKLGSFLEVPPGAVAREVEGTLDQPLERPGTQIGPYKLLQQIGEGGMGVVYMAEQKEP
                     VERRVALKIIKPGMDTRQVIARFEAERQALAMMDHPNIAKVLDAGTVGGIQNSERRMQ
                     KAEGTGSILNSEFCILNSAGRPYFVMELVRGVPITQYCDEHHLTPRQRLELFLPVCQA
                     IQHAHQKGIIHRDIKPTNILVAEYDQQAVPKVIDFGVAKAIGSPLTEKTMFTGFGQLV
                     GTLEYMSPEQAKVNQLDIDTRSDIYSLGVLLYELLTGSTPFDKQRLRSAAWDEMLRII
                     REEEPEKPSTRLSSVDALPS"
     gene            complement(1155..1712)
                     /locus_tag="VFV87_21755"
     CDS             complement(1155..1712)
                     /locus_tag="VFV87_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5106466.1"
                     /translation="MSDVTQLLSAIDAGDPAAAEQLLPLVYDELRKLAAARLAAEKPG
                     QTLQATALVHEAYLRLVGAEQAQQWDSRGHFFAAAAEAMRRLLVESARRKQSLKRGEG
                     LIRQPLDELEIAAPESRYDVLALDEALDQLAAADPQAAELVKLRFFAGLTAEQAAAAL
                     GVSARTADRIWSYARAYLFKCLAAS"
     gene            1920..>2884
                     /locus_tag="VFV87_21760"
     CDS             1920..>2884
                     /locus_tag="VFV87_21760"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5106467.1"
                     /translation="MPRWLLIFALASAWGWPLALFAADDAGLEFFEKQVRPLLVSRCY
                     ECHSAQSKKLQGGLRLDSLAAATKGGETGPAVVPGKPKESLLVDAINYGELYQMPPKS
                     KLPAEEIAVLTKWVEMGAPWPNEEPTTTGSGAAAEFDLTLRKASHWCWQPITKPPVPE
                     IRNPQSAIHNPIDAFILAKLQAARLSLAPPADKRTLLRRAYFDLIGLPPTPEEVEAFL
                     QDQSPQAFEKVVNRLLDSPHFGERWGRHWLDLVRYADTAGDGSDYPVREAYKYRDYVI
                     DAFNTDMPYDQFLCEQIAGDILASETPEVRYQERVIATGYIAVTKR"
BASE COUNT          495 a          940 c          922 g          527 t
ORIGIN      
        1 atcgaaggga gcgcatcgac gctcgatagc cgcgtgctgg gtttctccgg ttcttcctcg
       61 cggatgatcc gcagcatctc gtcccaggcg gcggagcgga ggcgctgttt gtcgaagggg
      121 gtgctgccgg tgagcagctc gtagagcagc acgccgagcg aatagatgtc gctgcgggtg
      181 tcgatgtcga gctggttcac cttcgcctgc tcgggggaca tgtactccag cgtcccgaca
      241 agctgcccaa agccggtgaa catcgtcttt tcggtcaagg gcgagccgat cgctttggcc
      301 acgccgaaat cgatcacctt ggggaccgcc tgctggtcgt attcggcgac gaggatgttc
      361 gtcggcttga tgtcgcggtg gatgatcccc ttctggtgcg cgtgctggat cgcctggcag
      421 accggcagga agagctccag ccgctggcga ggggtgagat gatgctcgtc gcagtactgc
      481 gtgatcggca cgccgcggac gagctccatg acgaagtagg ggcggccagc cgaattcaga
      541 atgcagaatt cagaattcag aatcgaacca gttccctcgg ctttctgcat tctgcgttct
      601 gaattctgaa ttccccccac cgttcccgca tccagcacct tggcgatgtt cggatggtcc
      661 atcatcgcca gggcctggcg ctcggcctcg aagcgggcga tcacctgccg cgtgtccatc
      721 cccggcttga tgatcttgag ggccacccgc cgctcgaccg gctccttctg ctcggccata
      781 tagaccacgc ccatgccgcc ctcgccgatc tgctgcagga gcttgtacgg cccgatctgc
      841 gtgccaggac gctccagcgg ctgatcgagg gtgccttcga cttcgcgggc aacggccccc
      901 ggcggaactt caaggaagct tcccagcttc agatgagctt cgagcaaacc ggcaatgtgg
      961 tcccggcggg ccggatcgcc gccgcacgcc tcgtccagcc aggccgcccg gccggctgga
     1021 tcggcgattg ccaacgctcg gtcgaagatc tcgcgttcgg tcatggcaag gcacctgtat
     1081 gcagaaggca atgcgaattc gccgcgcggc gtccgccaaa aaagtcgcgt tggcagcgcg
     1141 gaattgccgg gcagctacga agcggccagg cacttgaaca aataggctcg cgcgtaactc
     1201 caaatccgat cagcagttcg ggcagagacc ccgagcgccg cggccgcttg ttcggccgtc
     1261 aggcccgcga agaagcgcag cttgaccagc tcggcggcct ggggatcggc cgccgcgagc
     1321 tgatcgaggg cttcgtccag ggccagcacg tcgtagcggc tttcgggggc ggcgatttcc
     1381 agctcgtcga gcggctggcg gatgagtccc tcgccccgct tgaggctttg cttgcggcga
     1441 gcactttcga cgagcagccg tcgcatcgct tcggccgcgg cagcgaagaa atggccccgc
     1501 gaatcccact gctgggcctg ctcggcgccc accagccgca gatacgcctc atggaccagg
     1561 gccgtcgcct gcagggtctg ccccggtttc tcggctgcca ggcgcgccgc cgccagcttc
     1621 cgcagctcgt cataaacgag cggcagcagt tgctctgccg cggcgggatc gccggcgtcg
     1681 atcgccgaga gaagctgcgt gacatcgctc attgagcgtc accatacgcc tgcccgcggc
     1741 gaaatgccag ttcgcctccg gcagaggcag ggcgaattgg cggcgacggg tggcgtcctg
     1801 cttttgaccc ttctgctcgg ctcgtttaga cttgaatgct gttgccttgc ctgccttggg
     1861 cgcggggcga attgccgtcg ccctccttcc tgcccggcca tcggttcgga gtgaacttaa
     1921 tgcctcgctg gctgctgatc ttcgcgttgg catctgcctg gggctggcct cttgctttgt
     1981 ttgcggccga cgacgccggc ctggagttct tcgagaagca agtgcggccg ctgctcgtca
     2041 gccggtgcta tgagtgccac agcgcgcaga gcaagaagct gcagggaggc ctgcggctcg
     2101 attcgctggc ggcggccacg aaggggggcg aaacggggcc ggccgtcgtg ccgggcaagc
     2161 cgaaagagag cctgctggtc gatgcgatca attacggcga gctctaccag atgccgccga
     2221 aatcgaagct gcccgcggag gagatcgccg tcctgacgaa gtgggtcgaa atgggggccc
     2281 cgtggccgaa cgaggagcca accacaaccg gcagcggcgc ggcggcggaa ttcgacctca
     2341 ccctgcgcaa agcgtcgcac tggtgctggc agccgatcac caagccaccg gtgccggaaa
     2401 tccgcaatcc gcaatccgca atccacaatc ccatcgacgc cttcatcctc gccaaacttc
     2461 aagctgcccg tctctcgctc gccccgccag ccgacaagcg cacactcctc cgccgggcgt
     2521 atttcgatct catcggcctg ccgccgacgc cggaggaggt ggaggctttt ttgcaggacc
     2581 aatcgccgca agcgttcgag aaggtcgtta atcggctcct cgactctccc cacttcggcg
     2641 aacgctgggg ccggcattgg ctcgacctgg tgcgctatgc cgatacagcc ggcgatggtt
     2701 ccgattaccc cgtacgcgaa gcgtacaagt atcgagacta tgtcatcgat gccttcaaca
     2761 ccgacatgcc gtacgatcag ttcctttgcg aacaaatcgc cggcgatatt ctggcaagcg
     2821 aaacgcccga ggtgcgctac caggagcgcg tgattgccac cggctacatc gcggtgacga
     2881 aacg
//
LOCUS       DASUGB010001145         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1515728, whole genome shotgun sequence.
ACCESSION   DASUGB010001145 DASUGB010000000
VERSION     DASUGB010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515728"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1233
                     /locus_tag="VFV87_21765"
     CDS             184..1233
                     /locus_tag="VFV87_21765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5106468.1"
                     /translation="MAELRWERYPRVADHATARKWIGIEANLGLAPNTIHAYARALED
                     YLAFSATAPVEISDANREHIAAYVHHLASRRNSRPPNVVVIDSGVGLANATLQQRLTA
                     IRLFYDYLIEEGHRVSNPVGRGRYTATRCFGGARERGLIPRFKKLPWIPNDDQWRAVI
                     EATKREPLRNRVMLALSYDAALRREELCSLQTGDIDPSRRTISIRAENTKNRRARVVP
                     YSEATSKLYGAYLRKRRALSTDRGLLFLSESRRNWARPITIWTWSKVVEGISMRAGVP
                     EFTTHTSRHLCLTDLARASWDIHEIATFAGHRSIESTLLYIHLSGRDLAAKLARGMAA
                     IHDWRVRTIAEILHE"
     gene            1226..3499
                     /locus_tag="VFV87_21770"
     CDS             1226..3499
                     /locus_tag="VFV87_21770"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5106469.1"
                     /translation="MNEVISAARVEEAGDQWRWPIDASVYDRASRLYPREKNELAQMV
                     SCKRFGCWSQRTQEVLSRLRSPVYDVMKTIEAPAGHWCTATTVLLLETHRRKKSFWGW
                     TEAQWIETIGTSPSEFCARHSLADDTVRPYLAAVAYLFGCFQDVSSLGTINYVNLARH
                     VFGASTVNAAIRRAVDMTTAWGFGKCVAADLRTTIGILVLANRSPNLRDITVPFLEQQ
                     RRLAVNGRRSASILNLSRVLLTLGYTGAALPYFPETYKPKPVGDVREGVPLEWVQWVE
                     RWRATSTLQPKTRRMCCQYLFKCGRWLANVHPECSSPAAWTREIAADWVAAVCRMKVG
                     DWMQADQRYQRQRIGKPLGASTKGTHLSSLSVFFRDCQEWEWIPRRFDPRRCFAIPRS
                     IRALLAPNPRIIADDIWAKLLWAGLNLAASDLPVKPRQKCHYYPVEMVKAVTIVWLFC
                     GLRMDEIRRLRLGCVLSSKAPDDLTAVCTVSVPVNKTTAAFTKPVDQLVGEAVKGWEA
                     IRPPQPLTLDEKTGELVDFLFMFRGKRIAERYINETLIAILCQKAGIPTADARGNITS
                     HRARSTIASQLFNAKEPMSLFELQKWLGHKWAYSTEYYLRIGATKLAESYRNAEYFAR
                     NIRAIEVLIDRDIVLNGKANQEPWTYYDLGHGYCSYDFFDQCPHRMACAKCSFYIAKG
                     STQSLLLEGKSNLLRMRQEIPLKEAELAAIDDGVAAFEKLLAQLADVPTPAGPTPRQL
                     HASGLVQIAGASAPEGQ"
     gene            3496..>3721
                     /locus_tag="VFV87_21775"
     CDS             3496..>3721
                     /locus_tag="VFV87_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106470.1"
                     /translation="MKATWVDFRSVKARVTMEMALASYGVMLHRVDPSYLRGRCPLPT
                     HSSKTSAQSFIVNTDKNAWVCHSDSCSAARS"
BASE COUNT          758 a         1112 c         1108 g          743 t
ORIGIN      
        1 gcgtaagtaa tcgtgaatcg actgtggaac cgacataaga ttcccttttt gagatcgagt
       61 tttcagccgg tccggctggc aacgggcgca tggacgaaag atcacggtgt ggagatcttc
      121 gttcctcatc tgtggcgcga cttctgggga tctcctccgt ctaatccgca acggagcgca
      181 gcggtggcgg aacttcgatg ggaacgatat ccacgagtcg ccgaccacgc tacggcccgg
      241 aaatggattg gaatcgaggc gaaccttgga ttagcgccaa acacgatcca tgcttatgcc
      301 agagcccttg aggattacct cgccttcagc gctactgcac ccgtcgaaat ctcagacgcc
      361 aaccgcgagc atattgctgc ctacgttcac catctggctt cgcggcggaa ttccagacca
      421 ccgaatgtcg tcgtcatcga ttccggggtt gggttggcga atgccacatt gcaacaacgg
      481 cttactgcaa tccgactgtt ttacgattac ctgatcgagg aggggcaccg cgtcagcaat
      541 ccagtcggtc gcggcaggta cacggcaacg cggtgttttg gcggcgcgcg agagcgcggc
      601 ctcatcccac gtttcaagaa actgccctgg atccctaacg acgaccaatg gcgagctgtc
      661 attgaagcaa ccaagagaga gccgcttcgc aatcgtgtca tgctcgcgct gtcctatgac
      721 gcagcccttc ggcgcgagga actctgctcg cttcaaacag gtgacatcga tccgtcacgg
      781 cggacgatca gtattagagc ggaaaatacc aagaaccgcc gggcaagagt cgttccgtat
      841 tcggaagcga cgtccaagct atatggggca tacctgcgaa aacgacgcgc actcagcacg
      901 gatcgtggcc tgctgttcct ctccgagtcc cggcgcaatt gggcgcggcc gatcaccatc
      961 tggacgtggt ccaaggtcgt tgagggcatc tcgatgcggg ccggagtccc agagttcacg
     1021 acgcatacgt cgaggcatct ctgcctgacc gacctcgcac gtgctagctg ggacatccac
     1081 gagatcgcaa ccttcgccgg gcaccggagc atcgagtcta cgctcctgta tatccacctc
     1141 agtgggcgcg acttggccgc caagctggcg cgtggaatgg ccgctatcca cgactggcga
     1201 gttcgcacga ttgccgagat cctccatgaa tgaagtaata tccgcggccc gggtggagga
     1261 ggcgggagac caatggcgct ggccgattga tgcatccgtc tacgatcgcg ccagtcgcct
     1321 gtatccgcgt gagaagaacg agcttgccca gatggtgagc tgcaagcgct tcggatgctg
     1381 gtcgcagcgc actcaggagg tcctgtcccg attgcgcagc ccagtctatg acgtgatgaa
     1441 aaccatcgag gctcccgcag gacattggtg cacggcaacc accgtactct tgctcgagac
     1501 gcaccgccgc aaaaagagct tttggggctg gaccgaagct cagtggattg aaacaattgg
     1561 cacgtcgcca tcggaattct gtgcgcggca ctccttggct gacgatacgg tgcgcccata
     1621 cctggcggcc gtcgcatacc tttttggatg ctttcaggat gtctcgtcgc tcggaacgat
     1681 caattatgtg aacctcgcgc gccacgtatt cggcgcctcc accgtcaacg ccgcgattcg
     1741 acgggccgta gacatgacca cggcgtgggg cttcggtaaa tgcgtggccg ccgatctgcg
     1801 gaccaccatc ggaatcctgg tgctggccaa tcgtagtcca aacctcaggg acattaccgt
     1861 gcctttcctg gagcagcaga ggcgcctcgc cgtgaacggc cgccggagcg ccagcatcct
     1921 caatctttcg agggtcctct tgacgctcgg gtatacgggc gctgctctgc cgtactttcc
     1981 tgaaacctac aagcccaagc cggttgggga cgtacgcgag ggtgtccccc ttgagtgggt
     2041 tcaatgggtc gaacgctggc gagcgacgtc gacgcttcaa ccgaagactc gccgcatgtg
     2101 ctgccagtac ttattcaaat gcggccgctg gctggcgaac gtgcatccag agtgctcatc
     2161 gccggcggcg tggacccggg agatcgccgc ggattgggtg gctgcggtgt gccgaatgaa
     2221 ggtcggcgat tggatgcagg cggaccagag gtatcagcgt caacgtatcg gcaagcccct
     2281 cggggcgtcg accaagggca ctcatctaag ttccctgagc gtgttttttc gggactgcca
     2341 ggagtgggaa tggattcctc gccgctttga cccccgccgg tgtttcgcta tccctcggag
     2401 catacgtgct cttctggccc cgaatccccg aatcatcgcc gatgacatat gggcaaagct
     2461 cttgtgggct ggcctgaacc tggcggcttc cgacctcccg gtcaagccga ggcagaaatg
     2521 ccattactat ccggtggaga tggtcaaggc tgtgaccatc gtgtggctct tctgcggact
     2581 gcgcatggac gaaattcgtc gcctgcgact tggctgcgtc ctttcctcga aagctccgga
     2641 tgatctcaca gcagtttgca ctgtctcggt cccggtgaac aagacaaccg cggcgttcac
     2701 gaaacccgtg gaccaactcg tgggcgaggc tgtaaagggt tgggaggcca tccgtccgcc
     2761 gcagccgctg accctcgacg aaaagaccgg ggaactggtg gatttcctat tcatgttccg
     2821 cgggaagagg atcgcggagc gttacatcaa tgagacgttg atcgcgattc tatgccagaa
     2881 ggccggcatc ccaacagccg acgccagagg gaacatcacc agccaccgcg ctcgctcaac
     2941 gatcgcgtcc cagctcttta acgccaaaga gccgatgtcg ctcttcgaac tgcagaagtg
     3001 gctcgggcac aagtgggcct attcgaccga atattatctg cggattggcg cgaccaagct
     3061 ggccgagtcg taccgcaacg ccgagtattt cgcgcgcaac atccgggcca ttgaggtcct
     3121 tatcgaccgg gacatcgtcc tgaacggaaa ggccaatcag gaaccctgga cgtactacga
     3181 cctgggccac ggctattgtt cttacgattt ctttgaccag tgcccgcatc gaatggcctg
     3241 cgcgaaatgc agcttctaca tcgcgaaagg cagcacgcaa agcttattgc tcgagggcaa
     3301 gtcgaatctg ctgcgcatgc gccaggagat tccattgaaa gaagcggagt tggccgccat
     3361 cgacgatggc gttgcggctt tcgaaaaact cctggcccag cttgcggacg tccccacgcc
     3421 ggcgggtcct acgccgcgcc agttgcatgc ttccggcctc gtccagattg ctggtgcgtc
     3481 ggcgccggag gggcaatgaa agccacttgg gtggattttc gaagcgtgaa ggcgcgcgtc
     3541 accatggaaa tggctctggc cagctacggc gtaatgttac atcgcgtgga cccgagctac
     3601 ctccgcgggc ggtgtcctct tccaacgcat agttccaaga ccagcgcgca gagcttcatc
     3661 gtcaacacgg acaagaatgc ctgggtgtgt cattcagact cctgctcggc ggcgcgaagc
     3721 g
//
LOCUS       DASUGB010001146         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_822915, whole genome shotgun sequence.
ACCESSION   DASUGB010001146 DASUGB010000000
VERSION     DASUGB010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822915"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /gene="rho"
                     /locus_tag="VFV87_21780"
     CDS             <1..800
                     /gene="rho"
                     /locus_tag="VFV87_21780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5106471.1"
                     /translation="TTRVMDLLTPLGRGQRALLVAPPRSGKTILLQHMSMAMTANYPE
                     VKQFILLIDERPEEVTDMRRNVKGEVCASSLDEDIESHVRLSQLVIERCKRLAEMGQD
                     VFVLLDSITRLARAFNKYVGNKGEGIQSGGLSVRAMDIPKKLFATARAFEEGGSVTIA
                     GTALIETNSRMDDAIFQEFKGTGNMELVLDRKLAERRVYPAIDVSQSGTRREELLLDA
                     DTLHAVTMLRRTLSSMNPVDAMEQLTKQLSRFKTNYEFIKLISSARE"
     gene            complement(920..1783)
                     /locus_tag="VFV87_21785"
     CDS             complement(920..1783)
                     /locus_tag="VFV87_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106472.1"
                     /translation="MPLRPLLASMILLSAVGCARLATQPSEPSKLPPARMSPDSVVLE
                     VAFVRLPALDRAAYDAIWDQADEQHFPAELRRELGVNGLRAGVLGQQIPAELRELLDA
                     APNALEERSEDVTTSDVEVNRAPRRLQCRSGRRAKIIVSKTHSFLALLLNEQQQVRGH
                     ALTDAQCLLALKAYPQGNGRVKLDITPEVEHGQHRTEWVGSEGSLMQRVGRDKLVVDR
                     LRMDALLAPGQTLVISNTAEIKGLGEYYFAETAGGATERTLLLVRVAQTQIDDLFTPD
                     SVLTPLVTPGE"
     gene            complement(1867..3327)
                     /locus_tag="VFV87_21790"
     CDS             complement(1867..3327)
                     /locus_tag="VFV87_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106473.1"
                     /translation="MLTLAEEVGLGGYYLASRVRQAFLSLPEAEVAGLMARVREESAR
                     RHMVYLRDGQQEVIGVFPLPLTALPDQMAYLHLVSQTVASALKRLPELYFQDFAVREA
                     LRLPPDEEEWLWQCWGPSQRDNNPVFGRLDAVIDFTSPMWKNSLKFMEPNMSGIGGVY
                     LVPTAERVVQEVVLPRLVQRDSELHLELAPDIRELLMQAIRDHLEAIGRPARNICFVE
                     AKYAGSGIDEQEDLARYYHDQYGIKVMHADPGELELKDGEVYYAGDVVDLAYRDYSVA
                     DLLAQSQGGKNVQAMRELFQQNRMISSITAELDQKSCWEVLTDPAIAERHYSADERQI
                     FRRHILWTRIVSERKTTFPDGKEGDLLPFCRRERETLVLKPNRACGGEGVLIGPALSD
                     GQWNAALDQALADPARWVVQQLASIPVSEFPVVGPDGRIHSEPFHVVMGFAPSQYGVA
                     VLGRASQKQVVNVAQRGGMCVVFVGRPAGRLHGPRG"
     gene            complement(3467..3799)
                     /locus_tag="VFV87_21795"
     CDS             complement(3467..3799)
                     /locus_tag="VFV87_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106474.1"
                     /translation="MGHSEMCLAVAGLKPQQVDALTRRLAGDWSDFPPAERAALNFAR
                     QQCTDPALITDDDVGELVRHFGPRRAWQVIFWASRCHYMTKVADAFQLPLEKDNPFWN
                     MPGAKRAT"
     gene            complement(3800..>4560)
                     /locus_tag="VFV87_21800"
     CDS             complement(3800..>4560)
                     /locus_tag="VFV87_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106475.1"
                     /translation="ARLRGHVRWAAAEANGCAYAQAVAEADLLEAGCTDVDCKRLAGS
                     PDFLPPVDRAAVAFARKMMRRAQTVTDEEVQQLIDLLGEERVVALVALLAHASFQDRL
                     FLALGLGPELDAALPPQAARFVLPPPVPPPAGSSQPPARGNPEDDDEPGGKQPPEDNR
                     WSDFQERLEEQRKRTARIRVPSQDEVIQRIGAQHPSQWQSEILWSRVCYGYQPELTDA
                     WFSTVAAYRQESSLDPLLQQSIFWVVTESLECFY"
BASE COUNT          798 a         1596 c         1407 g          759 t
ORIGIN      
        1 tgaccacgcg ggtgatggac ctgctcacgc ccctcggccg cggccagcgg gccctgctcg
       61 tcgccccgcc ccgcagcggc aagacgatcc tgctgcagca catgagcatg gcaatgaccg
      121 ccaactatcc ggaggtcaag cagttcatcc tgctgatcga cgagcggccg gaagaagtga
      181 ccgacatgcg gcgaaacgtc aaaggcgagg tctgcgccag cagcctcgac gaagacatcg
      241 agagccacgt gcgcctgtcg cagctcgtca tcgagcgctg caagcggctg gctgagatgg
      301 gccaggacgt gttcgtgctg ctcgactcga tcacccgcct cgcccgcgcc ttcaataaat
      361 acgtgggcaa caagggcgaa ggtatccaat ccggcggcct cagcgtccgc gcgatggaca
      421 tcccgaaaaa gctcttcgcc acggctcgcg cgttcgagga agggggctcg gtgacgattg
      481 ccggcaccgc cctgatcgaa accaacagcc gcatggacga tgcgatcttc caggagttca
      541 aaggcacggg caacatggag ctggtactgg accgcaagct cgccgagcgg cgggtttatc
      601 cggcgatcga cgtttcgcag tccggcacgc ggcgcgaaga gctgctgctc gatgccgaca
      661 cgctgcacgc ggtgacgatg ctccgccgga cgctcagcag catgaacccc gtcgatgcga
      721 tggagcagct caccaagcag ctcagccgct tcaagacgaa ctacgagttc atcaagctga
      781 tctcgagcgc ccgcgagtaa tgcgtagggt gggatgtgcc caccagttcc cgtagggtgc
      841 ataagcgaag cgcaatgcac catcgcgcgg gtctgtgcgc acaacccggt gcatttcggc
      901 cgcggcggcc tcatgcaccc tactcccccg gcgtgaccag cggcgtgaga acgctgtcgg
      961 gcgtaaacag atcgtcgatt tgcgtctgcg ccacgcgcac cagtagcagc gtccgctccg
     1021 tcgcgccacc ggccgtttcg gcgaagtaat actcgcccag ccctttgatt tccgccgtgt
     1081 tggaaatgac cagcgtctgg cccggcgcga gcagcgcgtc catgcgcagg cgatcgacca
     1141 ccagcttgtc gcggccgacg cgctgcatca gcgatccttc gctgccaacc cactcggtcc
     1201 gatgttgccc gtgctcgact tcgggcgtga tgtcgagctt cacgcggccg ttgccctgcg
     1261 ggtaggcctt gagcgccagc aggcactggg cgtcggtcag cgcgtgcccg cggacctgct
     1321 gctgctcgtt taagagcaat gcgagaaacg aatgtgtctt ggaaacgata atcttcgccc
     1381 gtcggccgct gcggcactgc aggcgtcgcg gggcgcgatt gacctctacg tcgctcgtcg
     1441 tcacgtcttc gctgcgttct tccagcgcgt ttggcgccgc gtccaacagc tcgcgcaatt
     1501 ccgccggaat ttgctggccg aggactccgg cccgcaatcc gttgacgccc agctcgcgcc
     1561 gcaattccgc ggggaaatgc tgctcgtcgg cctggtccca gatcgcgtcg taggccgcac
     1621 ggtccaaggc cggcaaccgc acgaaggcca cttccagcac cacgctgtcg ggcgacatcc
     1681 gcgccggcgg cagcttgctc ggctcggatg gctgcgtcgc cagccgcgcg cagccgaccg
     1741 cgctcagcaa gatcatcgaa gccagaagcg ggcgcagcgg catccgtgcc gtccgggaga
     1801 agcggagtag agccgcggag cgtatcgcgg caatctgacg cagtcaatgc gaactgcccc
     1861 tgacggctat cccctcggcc cgtgcaatcg ccccgccggc ctcccgacaa acacgacgca
     1921 catcccgccg cgctgggcga cgttcaccac ctgtttttgt gaggcccgtc ccagcaccgc
     1981 cacgccatat tgcgagggag caaagcccat cacgacgtgg aacggctcgc tgtgaatgcg
     2041 cccgtccggc ccaacgaccg gaaattcgct cacggggatg ctagcaagct gctgcacgac
     2101 ccaccgcgcc ggatcggcca gcgcctggtc cagcgccgca ttccactggc cgtcgctcag
     2161 ggccggaccg atcagcaccc cttcgccgcc gcaggcccgg ttcggcttca ggaccagcgt
     2221 ctcccgctcg cggcggcaaa acggcagaag gtccccttcc ttgccgtcgg ggaacgtcgt
     2281 cttccgctcc gagacgattc tcgtccataa aatgtgccgc cggaaaatct gccgctcgtc
     2341 ggcgctgtag tgccgctcgg caatcgccgg atcggtcagc acctcccagc agcttttctg
     2401 gtccagctcg gcggtgatcg acgagatcat ccggttctgc tgaaacagct cgcgcatggc
     2461 ctggacgttc tttccgcctt gcgattgagc cagcaagtct gccaccgaat aatcgcgata
     2521 tgccagatcg accacgtcgc ccgcgtaata gacctcgccg tccttcagtt ccaattcgcc
     2581 cgggtcggcg tgcatcacct tgatgccgta ctggtcgtga tagtagcggg ccaggtcttc
     2641 ctgctcgtcg atgccgctgc cggcgtactt cgcctcgacg aagcagatgt tccgcgccgg
     2701 ccggccgatc gcctccaggt ggtcgcggat cgcctgcatc agcagctcgc ggatgtccgg
     2761 cgccagctcc aggtgcagct cggaatcccg ctgcaccagc cgcggcaaca ccacttcctg
     2821 cactacccgc tcggcggtcg gcaccaggta caccccgccg atgccgctca tgttcggctc
     2881 catgaacttc agcgagttct tccacatcgg gctggtgaag tcgatcaccg catccagccg
     2941 gccgaagacc gggttgttgt cgcgctggct cggcccccag cattgccaga gccactcttc
     3001 ctcgtcaggc ggcagacgga gcgcctcgcg gacggcgaaa tcctggaaat agagctccgg
     3061 caggcgcttg agggcgctag cgaccgtctg cgaaaccagg tgcagatacg ccatctggtc
     3121 gggcaacgcc gtcaaaggca gcggaaagac gccgatcacc tcctgctgcc cgtcgcgcag
     3181 atacaccatg tgccgccggg ccgattcctc ccgcacccgc gccatcaacc ccgccacttc
     3241 cgcctccggc agcgagagaa acgcctggcg cacgcggctc gccaggtaat agcctcccag
     3301 ccctacttcc tcagcaagcg tcaacatcag agatccccgc ctctcgcttg tcagccgtcg
     3361 accttcgcgt gcctcctgtc agcgcccgca ttctagcggc tcgctcctcc gcccatagct
     3421 tccctcggcc gccgggcaat atttagtagc cgcggcggcg gagccgctag gtcgcccgct
     3481 tcgcacccgg catgttccaa aaggggttgt ctttctcgag cgggagctgg aaggcatcgg
     3541 ccactttggt catgtaatgg cagcgcgagg cccaaaagat gacctgccac gcgcgtcgcg
     3601 ggccgaagtg ccgcacgagc tcgccgacgt cgtcatcggt gatcaaggcg ggatcggtgc
     3661 actgctggcg ggcaaagttc agcgccgctc gttccgccgg agggaaatcg gaccaatcgc
     3721 cggccagccg ccgggtgagg gcgtccactt gctgcggctt cagccccgcg accgcgaggc
     3781 acatttcgga gtgccccatt cagtagaaac actccagcga ctcggtcacc acccagaaga
     3841 tcgactgctg aagcagcggg tcgagcgacg actcctgacg gtaagcggcg accgttgaga
     3901 accaggcgtc ggtcagctcc ggctgatagc cgtagcaaac gcggctccac agaatttcgg
     3961 attgccattg gctgggatgt tgtgcgccga tccgctggat cacctcgtcc tgggaaggaa
     4021 cgcgaattct ggccgtccgc ttgcgctgct cttcgaggcg ctcctgaaaa tccgaccaac
     4081 gattgtcctc cggtggctgc ttgccgccgg gctcgtcgtc gtcttcagga tttccgcgcg
     4141 ccggcggctg ggacgacccg gccggcggcg gcacaggcgg cggcaaaacg aacctcgcgg
     4201 cctgcggcgg cagcgctgca tccagctccg ggccgaggcc cagcgccagg aacaggcggt
     4261 cctggaagct ggcgtgggcc aagagcgcga cgagcgctac gacgcgctcc tcgcccagaa
     4321 ggtcaatgag ctgctgcact tcctcgtcgg tgaccgtctg ggccctgcgc atcatcttgc
     4381 gggcgaaggc caccgccgct cgatcgaccg gcggcagaaa atcgggactg ccggccagcc
     4441 gcttgcaatc aacatccgtg cagccggcct cgagcagatc ggcctcggca accgcctgag
     4501 cataggcgca gccgttggcc tctgccgcag cccagcggac gtgaccgcgc aagcgcgcat
//
LOCUS       DASUGB010001147         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1100687, whole genome shotgun sequence.
ACCESSION   DASUGB010001147 DASUGB010000000
VERSION     DASUGB010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100687"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2864
                     /locus_tag="VFV87_21805"
     CDS             <1..>2864
                     /locus_tag="VFV87_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106476.1"
                     /translation="VDPVAPAAVNNPRPQPGINPATAPQPTPTVSTPAQPAAAPASTN
                     SGNNANNNSSSSSDSGDNPFATAETVALPDRPTGLPKGGEDIENPFGDQAVVNQPAVA
                     APTDADLTKPAGWSARPDPPPAPIEYKKGKISIPFPDRAELRLPHGPSHFALGTIDNF
                     QASAFQVFDLRNGKSIGKEVMTASEFGRQTEVFSPDGRYLAAIQRPQGEAETTPVFVW
                     SFITGDVHRTLELDGKHVNGQLRFGAPHQLISLHNPDWENYVLTVWDLQTGNKLKEIR
                     LPHGRGENQLRPESLSISPGGRYACLLIGKQLGAIDLTTGEPAGAITLGETPSSCEGT
                     MFSPDGGELAVVVSQGSGHHLYIFDFASGRVLLDKDYRGRLQHFFYNGPTIDWLPDKS
                     GLVYAGHLLLERNTGEEVWVFPTTDSNPRRLITAGDILVLLREGRTKALKTEELPEKD
                     IAKAMQAVREGGAAVDSALPPISTADLFSATSATLPNGFVQWSVRPDISGTPPRGQER
                     EILIAKEGETVQRMIFAGPEAGKVVVQKEVTRKAGTGRAQTRQIVIERFDTTSGAKGN
                     TVPLPNVYQLADVSPSGNLAVVAFARESGKYDRLDVVGLSPKKHICAWRPFGSEPDKK
                     PDDPLRRHFGNWGWSNDPKLVVWASMIDDEHVLTINTAGKLVCWKIPECKAVYYFEDF
                     GEPLAMSAGRRYLAGGHFGEFRVFDALTGQCVGDLEAPALGSRAVRGGFRPDGEELAA
                     VIDAGPDKMLVRWNLADGKIVQEIPIPGVTVGFHSPFHFSHNGARAGLEYRGDNFLML
                     DDQYLINLDKRAVAWRYHLSRGYFAVNSPDQNTWYGIRKDNANDNSVFLTYMPTPSQS
                     VKNKSDLVSLEQQLVLFPGKSVRLHIDLNGVGMGELTPSVTKAVQQSLEARGFIVDPS
                     APLVFSMVAAQRSTGESVGVYESSSPFGFHSPIFGPRGNP"
BASE COUNT          520 a          955 c          916 g          473 t
ORIGIN      
        1 ccgtggatcc tgtcgcgccg gcggcggtca acaatccgcg gccgcagcct gggatcaacc
       61 cggcgaccgc accgcagccg acgcccacgg tttccacacc cgcgcagccc gccgccgcac
      121 cggccagcac gaatagcggg aacaacgcaa acaacaactc atcgtcgtcg tccgactccg
      181 gcgacaaccc gttcgccacc gccgagaccg tggcgcttcc cgaccgcccg accgggctgc
      241 ctaaaggggg cgaggacatt gaaaatccgt tcggcgatca ggccgtcgtc aaccagccgg
      301 cagtcgcggc gccgaccgac gcggatctga ccaagccggc cggctggtcc gcccggccgg
      361 atccgccgcc ggcccccatt gagtacaaaa aggggaagat ctcgatcccc tttccggacc
      421 gggccgaact gcgcctgcct cacgggccca gccattttgc cctcggcacg atcgacaact
      481 tccaagccag cgccttccag gtcttcgact tgcgaaacgg caagtcgatc ggcaaagagg
      541 tcatgacggc cagcgaattc ggccgccaga cggaggtctt cagccccgac ggccgctacc
      601 tggcggcgat tcagcgtccg cagggggagg cggagaccac gccggtgttt gtctggtcgt
      661 tcatcacggg cgacgtccac cgcacgctgg agctggatgg gaagcacgtc aacggtcagc
      721 ttcggttcgg cgcgccccat cagcttatct cgctccacaa tcccgactgg gaaaattacg
      781 tcctgaccgt ctgggatctg cagaccggca acaagttgaa ggaaatccgc ctgccgcatg
      841 gccgcggcga gaaccagctt cggccggagt cgttgtcgat cagcccggga ggacggtacg
      901 cctgcctctt gatcggcaag cagctcgggg cgatcgacct gacgaccggc gagccggcag
      961 gggcgatcac gctgggcgag acgccctcct cgtgcgaagg gacgatgttt tcgcccgacg
     1021 gaggggagtt ggcggttgtc gtgtcgcagg gttcgggcca ccacctttat atcttcgatt
     1081 tcgccagtgg ccgcgtcctc ctggataagg actatcgcgg gcggctgcag catttcttct
     1141 acaatggccc gacgatcgac tggctgccgg acaagagcgg cctggtctat gccggtcacc
     1201 tgttgctgga gcggaacacg ggcgaggaag tctgggtctt tccaacgacc gacagcaatc
     1261 cgcggcggct gatcacggcg ggcgacatcc tggtgctact ccgcgagggc cgcaccaagg
     1321 ccctcaagac cgaggaattg cccgagaaag acatcgccaa agccatgcag gccgtgcgtg
     1381 aaggtggggc ggctgtcgat tccgccctgc cgccgatttc cacggccgac ctgttcagcg
     1441 ccacctcggc gacgctcccc aatggctttg tgcagtggtc ggtgcggccg gatatttccg
     1501 gcacgccgcc gcgcggccag gagcgcgaaa tcctgattgc caaggaaggc gaaaccgtgc
     1561 agcggatgat ctttgccggg cccgaagcgg gcaaggtcgt cgtgcagaag gaagtcaccc
     1621 gcaaggcagg caccggccgc gcccagacgc ggcagatcgt catcgaacgg ttcgacacca
     1681 ccagcggcgc caaggggaat accgtcccgc tgccgaacgt ctatcagctc gccgatgtca
     1741 gccccagcgg caacctggcg gtcgtcgcct tcgcccgcga atcgggcaaa tacgaccggt
     1801 tggacgtcgt cggcctctcg cccaagaagc acatctgcgc ctggcggccg ttcgggagcg
     1861 agccggacaa gaagcccgac gatccgctgc ggcggcactt cggcaattgg ggctggtcga
     1921 acgatcccaa gctcgtcgtt tgggccagta tgatcgacga cgagcacgtg ctgacgatca
     1981 acaccgccgg caagctcgtc tgctggaaga ttcccgagtg caaggcggtg tattacttcg
     2041 aggacttcgg cgagccgctg gcgatgagcg ccggccgccg ctacctggcg gggggccact
     2101 tcggcgagtt ccgcgtcttc gatgccctca ccggccagtg cgtcggcgat ctggaagcgc
     2161 cggccctcgg cagccgggcg gttcgcggcg gcttccggcc cgatggcgag gagctggctg
     2221 cggtgatcga tgccgggccg gacaagatgc tcgtccgctg gaatctggcg gatggcaaga
     2281 tcgtccagga aataccgatt cccggcgtga ccgtaggctt tcattcaccg ttccactttt
     2341 ctcacaacgg cgcgcgtgcc ggattggagt atcgcggcga caatttcttg atgctcgacg
     2401 accagtacct catcaatctc gacaagcggg cggtcgcctg gcgctatcac ttgagccgcg
     2461 gctatttcgc ggtgaattcg cccgaccaga acacctggta cggcatccgc aaagacaacg
     2521 ccaacgacaa ctcggttttc ctcacgtaca tgcccacgcc cagccagtcg gtcaagaaca
     2581 agtccgacct ggtctcgctc gagcagcagc tcgtgctgtt tcccgggaaa tcggtgcggc
     2641 tgcacatcga cctgaacggc gtgggcatgg gggaactgac gccgtcggtg accaaggcgg
     2701 tgcagcaatc gctggaggcc cgcgggttca ttgtcgatcc cagcgcgccc ttggtgttct
     2761 cgatggtcgc cgcccagcgc tcgaccggcg aatcggtcgg tgtgtatgaa agcagctcgc
     2821 cgttcggctt tcacagcccg attttcggcc cgcgcggcaa cccg
//
LOCUS       DASUGB010001148         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1516127, whole genome shotgun sequence.
ACCESSION   DASUGB010001148 DASUGB010000000
VERSION     DASUGB010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516127"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="VFV87_21810"
     CDS             complement(<1..2153)
                     /locus_tag="VFV87_21810"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5106477.1"
                     /translation="MSVASNTTTGLRISPAVVAAREELAQGRAKLRRQHESGSPGVQV
                     CVHFTDLLEAIVLRLFQDALDTLPEPARQRAAQATAVVAHSGFGRREMSPFSDIDVML
                     LHASGCGELVKPLIRRFSQNLYDTGMEIGFAARTASEACRLALDDATILTSLSESRLV
                     GGEASLFAAFDGKFRRMVKRRWRRLVGKAEEARRDERTQYGETVFLLEPNVKRSRGGL
                     RDLQFIRWVGFVRYGENDIDALAHAGWLTKEEQKKLRDARDFLLWLRNDLHFAAGKAN
                     DILERTEQLRLAESRRYLEVAGLLPVEQFMREYFQRTCDVRDIAAHFALAAQPRSLLR
                     SIEPYLSRNLEGFRVGPRTIAATREGLDRLRTDLAEVLRLLDAANRHNKRIDNDTWKA
                     IRTAMLQRGPADPDQILPAGVVEKFFTLLSEPERLGDSLRRLHELRVLEQFIPGMSHA
                     RGLLQFNAYHRYTVDEHSIRVVEHLTSFASDRGTPGDVYRSIKNKATLHLAALLHDLG
                     KGYVEDHSEVGARLAEQTATHLGLTEHDAQTLRFLVHKHLRMSHLAQQHDISDDNVVV
                     PFAVEVGSPEALKMLFVLTLADLAAVGPGVLNEWKQELLTDLYHHVAHLLASDSPAAA
                     ASERLRARRGEVLALSRRHDGVAWWETQVMSLPASCLFAASPEQIVDELERIRRLPRH
                     EALAWGRYLPGRNVVEYTVGTYEDISPGIFHRLTGA"
     gene            complement(2180..2518)
                     /locus_tag="VFV87_21815"
     CDS             complement(2180..2518)
                     /locus_tag="VFV87_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5106478.1"
                     /translation="MKKIEAVIRHFKLEDVKNALTERGIHGMTIAEVRGFGRQKGHTE
                     MYRGTEYAVDFVPKVKVEVVCSDANLALVIDTIMRAAQTGQIGDGKIFVTSLQDVVRI
                     RTGETGEDAL"
     gene            complement(2673..>3251)
                     /locus_tag="VFV87_21820"
     CDS             complement(2673..>3251)
                     /locus_tag="VFV87_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX5106479.1"
                     /translation="ELASDGLAASAFCATHFAAAAGTLSWAGMEWITRGKPSVLGAAS
                     GAVAGLVCITPASGYVQPMPAVAMGLAAGIVCFFACTKLKAAFGYDDSLDAFGVHGVG
                     GTLGAVLTGVFATIEVNNMNDGQAVGLIDGNASLLIGQIVAVIVTWLLAAVATFVILK
                     VLDVTMGLRVKREEELQGLDLSQHGEEGYIFI"
BASE COUNT          570 a         1029 c         1053 g          599 t
ORIGIN      
        1 gcgccagtca gcctgtgaaa gattccagga gagatatctt cgtacgtgcc gacggtgtac
       61 tcgacgacgt tccgacccgg cagatagcgg ccccaggcca gcgcttcgtg gcgaggcaat
      121 ctgcgaatcc gctccagttc gtccacgatc tgttccggcg aagcggcgaa caggcaactc
      181 gccgggagag acatgacttg tgtttcccac caggcaacgc cgtcgtgccg gcgggataat
      241 gcgagcactt cgccgcgccg cgctcgcagc cgctcgctgg cggctgccgc gggcgaatcg
      301 ctggccagca gatgagcgac gtgatgatac agatccgtga gcagctcctg cttccactcg
      361 ttgagaacgc cgggcccaac ggccgccagg tcggcgagcg tcagcacaaa cagcatcttg
      421 agtgcttccg gcgagccgac ctcgacggca aacggcacga cgacgttgtc gtcgctgatg
      481 tcgtgctgct gggcgagatg gctcatccgc aggtgcttgt ggacgaggaa ccgcagcgtc
      541 tgcgcgtcgt gctcggtgag ccccaggtgc gttgccgttt gctcggccag gcgagcgccg
      601 acttcgctgt gatcctcgac gtagcccttc ccgaggtcgt gcagcaaggc cgccaggtgc
      661 agcgtagcct tgttcttgat cgagcgataa acatcgcccg gcgtgccgcg gtcgctcgcg
      721 aaggaggtca gatgctcgac gacgcggatc gaatgctcgt cgacggtgta gcgatgatag
      781 gcattgaatt gcaacaggcc ccgcgcgtgg ctcatgccgg gaatgaactg ctccagcacg
      841 cgcagttcgt gcagccgccg cagcgaatcg cccagccgtt caggctccga gagcagcgtg
      901 aagaacttct cgacgacccc agcgggtagg atctgatccg gatcggctgg gccgcgctgc
      961 agcatcgccg tgcggatcgc tttccacgtg tcgttgtcga tccgcttgtt gtgacgattg
     1021 gccgcatcca gcagtcgcag cacttcggcc agatcggtgc gcaggcggtc cagaccctca
     1081 cgcgtggcgg caatcgtcct gggtccgacg cggaagcctt cgagattgcg gctgaggtag
     1141 ggttcgatcg accgcagcag cgagcgcggt tgggccgcga gagcgaagtg ggcggcgata
     1201 tctcgcacgt cgcaggtgcg ctggaaatac tcccgcatga actgctcgac gggtaggagt
     1261 ccggcgactt ccaggtagcg gcgtgactcg gccaaccgca attgttcggt tcgttccagg
     1321 atgtcgttgg ccttgccggc ggcgaagtgc aggtcgttcc gcagccagag cagaaagtcg
     1381 cgggcatcgc ggagcttctt ctgctcttcc ttcgtgagcc agccggcgtg ggccagggcg
     1441 tcgatatcgt tctcgccgta gcggacgaat ccgacccagc ggatgaattg caggtcgcgc
     1501 agcccgccgc ggctgcgttt gacgtttggc tccagcagaa agacggtctc gccgtactgc
     1561 gtgcgttcgt cacggcgggc ttcttccgcc ttgccgacca gccgccgcca gcggcgtttg
     1621 accatccgcc ggaatttgcc gtcgaaagcc gcgaacaggc tcgcctcgcc gccgacgagc
     1681 cgcgattcgg agagcgacgt gaggatggtg gcatcgtcca gcgccagacg gcaggcctcg
     1741 gacgccgtgc gcgccgcaaa tccgatttcc atcccggtgt cgtacaggtt ctgggagaag
     1801 cggcggatga gcggcttgac cagttcgccg cagccgctgg catggaggag catgacgtcg
     1861 atgtccgaga acggcgacat ctcgcggcgc ccaaagccgc tgtgtgcgac gaccgcagtc
     1921 gcctgggccg cccgctgccg ggcgggctca ggcagcgtat ccagcgcatc ctgaaacagt
     1981 cgcaggacga tcgcttcgag caggtccgtg aagtgaacgc aaacctgcac gcccggcgaa
     2041 ccgctttcgt gctgtcggcg gagtttggcg cggccctgcg ccagctcttc gcgggcggcg
     2101 acaaccgcgg gactgatgcg gagcccagtt gtcgtattcg acgcgacgct catgctcgat
     2161 ccggcgaaca atctcgggat cacagggcgt cttcacccgt ttcgccggtg cgaatgcgaa
     2221 cgacatcctg caggctggtg acaaagattt tgccgtcgcc gatctggccc gtctgggccg
     2281 ccctcatgat ggtatcgatt accagggcca gatttgcatc cgagcagacg acttcgacct
     2341 tcaccttggg cacgaagtcg acggcgtatt ccgtgccgcg atacatctcc gtgtgtccct
     2401 tttggcggcc gaatccgcgc acctcggcaa tcgtcatgcc gtggattccc cgctcggtca
     2461 gggcgttctt cacatcttcg agtttgaagt ggcgaatgac ggcttcgatc tttttcatag
     2521 ggaataggct ccagcgcgtg gggcagacat tcctgtctgc cgctgtttct gtttttctgg
     2581 gcgaccaccg gcaggcaaga tgcctgcccc acttttccgg caggcaagat gcctgcccca
     2641 ctttcgatat tctacgccaa accgaggcag ggttatatga agatatagcc ttcctcgccg
     2701 tgttggctga ggtcgaggcc ctggagttcc tcctcgcgtt tgacgcggag ccccatcgtc
     2761 acgtccagga ccttgaggat gacgaacgtg gcgacggctg cgaggagcca ggtgacgatc
     2821 accgccacaa tctggccgat cagcaggctc gcattaccgt cgatgaggcc gaccgcctgc
     2881 ccgtcgttca tgttgtttac ctcgatcgtg gcgaaaacgc ccgtgaggac ggctcctaac
     2941 gttccgccaa cgccgtgcac tccaaaggca tcgagcgagt cgtcgtagcc gaaggccgcc
     3001 ttcagcttcg tgcaagcgaa aaagcagacg attcctgccg ccagccccat ggccacggcc
     3061 ggcatcggct ggacatagcc ggatgccggg gtgatgcaga ccaggccggc cacggctccc
     3121 gaggcagcgc cgagcacgct gggcttgccg cgggtgatcc attccatgcc agcccaggag
     3181 agcgtgccgg cggcggctgc gaaatgagtc gcacaaaagg cgctggcagc caaaccatcg
     3241 gacgcaagtt c
//
LOCUS       DASUGB010001149         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1379177, whole genome shotgun sequence.
ACCESSION   DASUGB010001149 DASUGB010000000
VERSION     DASUGB010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379177"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VFV87_21825"
     CDS             complement(<1..1272)
                     /locus_tag="VFV87_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106480.1"
                     /translation="MPATESTWRNTKLLHHIFAVSGVVLTISTIWMLYKDHVRSWKTY
                     QSNAVNIDLKMTKMRQEQYETGDALVEHNRLAAALVTAQAQPAPAELLSQFQSQLESL
                     NKAIAEISQHHAYSEIAVDFRYIENTARRLDELAKAAQEKRQTATAAIAAADKAAGDS
                     SLSAEDRTKLEDAARKADREATAAEEKAGAVRAELIGYLQTAVAHARAREDKALGIRK
                     FKSADVDAAKANLDIAIRDNLSAAERQNREKDIQRLIEEPNKGLAALTDVYQTLSAGR
                     KRLEATLKAITAPVEKAKKAHEDSVAELDRLRKQYADDKETYFQLSLQYPFVFGKKIT
                     TLPIIDAFNSVWKIENLWSEDLVHKYGSFSEVRRFDRCTTCHQSMQKSQPGAPTEPLY
                     VRDKEFLVTLTPPPKDAPPVPRRDAYGKPLPL"
     gene            complement(1353..2732)
                     /locus_tag="VFV87_21830"
     CDS             complement(1353..2732)
                     /locus_tag="VFV87_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106481.1"
                     /translation="MHGQSLDVFLSQISGFLGSYYIFLALMTGVAAYLLWQRADSRPL
                     FRLPGVDFPVTPAIVWLLVSLGFVLIASLAYSGGPDVMAWISIPTAVRAGINWVMNPT
                     IYTLGTLVLLGIAFLLRRFLVKPWVAWSLLNVALLLMGMSMTDPNFFNIVGKPDNVPI
                     VAMVFLLGFFTWLAAYRAVQNDDRLRQGLEPLEKLDSEKVLVWPDLVYTELICMVALT
                     AVLLFWAIALQAPLEEPASAVKTPNPSKAPWYFLGLQEMLVYYDPWMAGVVLPSMIIA
                     GLMAIPYIDFNKQGNGYYTIDQRKFAYIMFQIGFLVLWVTLIVMGTFLRGPNWNFFGP
                     YEIWDAHKVEALNNVDLSEYFWVTLLGSSRPKAADDAGELTKILVALKRESPGIILVI
                     GYFTMVPPLLVVLSKFFQNLFIRMGFIRYMVMANLLLFMMLLPIKMLGRWTINLKYFI
                     SMPEYLLNF"
     gene            complement(2834..3610)
                     /locus_tag="VFV87_21835"
     CDS             complement(2834..3610)
                     /locus_tag="VFV87_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5106482.1"
                     /translation="MATLGEIIRESQIWKSIIRHPAPVDRRNRIVVMLTNFFLHLHPV
                     SIKKQGIALSFTWCMGGVTFFLFLVETITGVLLMFYYRPTLEWAYNDILALRDVTSLG
                     ILRELHRWGAHAMVITVWLHMYRVFLTGSYKPPREFNWVIGVILLLLTLLLSFTGYLL
                     PWDQLAIWAITVGSNMARATPFLGYEGPGNQLLTIDGIDMITNASDARFGLLGARFVG
                     EETLNRFYVLHCVAIPLAAAMLMAIHFWRVRKDGGISGPL"
     gene            complement(3718..>4425)
                     /locus_tag="VFV87_21840"
     CDS             complement(3718..>4425)
                     /locus_tag="VFV87_21840"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5106483.1"
                     /translation="KDTASILAAARKGAKPGPITKAEAAAKATPAAPAAKKKLDVPPM
                     PAKPDYARAPAPAAPIETATRRNFLAASAGVLFGSSLAIGFTSLAATHLMWLLGLARF
                     MFPNILIEPPTKFKVGFPDSLAPGQVETKYIPQFGVWIVRYEFDGQPMIFALKSVCTH
                     LGCTPNWLEAEQKFKCPCHGSGFYKDGVNFEGPAPRPLERYAISLADDGQLLVDKSRT
                     FQQELGQWRDTASFVTV"
BASE COUNT          867 a         1283 c         1423 g          852 t
ORIGIN      
        1 cagcggcaat ggcttcccgt aggcatcgcg gcgaggaacg ggcggagcgt ctttcggcgg
       61 cggcgtaagg gtgaccagga actccttgtc gcgaacgtag agcggctccg tcggagcgcc
      121 cggctggctc ttctgcatcg actggtggca ggtggtgcag cggtcgaacc gccgcacttc
      181 gctgaagctg ccgtacttgt gaaccaggtc ctcgctccac agattctcga tcttccaaac
      241 cgaattgaac gcgtcgatga tcggcagcgt ggtgattttc ttgccgaaca cgaatggata
      301 ctgcaaactg agctggaagt aggtctcttt gtcgtcggcg tactgctttc gcagccggtc
      361 gagctcggcg acgctatctt cgtgggcttt cttcgccttt tcgaccgggg cggtgatcgc
      421 cttgagcgtt gcttccagcc tctttcggcc ggcgctgagc gtttgataaa cgtcggtcag
      481 cgcggccaga cctttgttcg gctcctcgat cagccgctga atgtccttct cgcgattctg
      541 gcgctcggcc gcggagaggt tgtcgcggat ggcgatgtcg agattggcct tggcggcatc
      601 gacgtccgcc gacttgaact tgcggatgcc gagggctttg tcttcgcggg cgcgggcgtg
      661 cgcgaccgcc gtctgcaggt agccgatcag ctcggcgcgg accgccccgg ccttctcttc
      721 ggcggccgtg gcttcgcggt cggccttgcg ggcggcgtct tccagcttcg tgcggtcctc
      781 ggcggaaagc gaactgtcgc cggcggcttt gtcggccgcg gcaatggctg cggtcgccgt
      841 ctggcgtttt tcctgggcgg ctttggcaag ctcatccagc cgcctggccg tattctcgat
      901 gtagcggaaa tcgacggcaa tctcgctata agcgtggtgc tgcgagatct cggcgatggc
      961 cttgttcagg ctttcgagct gcgactggaa ttgcgagagc agctccgccg gagccggctg
     1021 ggcctgggcc gtgacgaggg cggcggccag gcgattgtgc tccaccaggg cgtccccggt
     1081 ttcgtactgc tcctgccgca tcttggtcat cttcaggtcg atattaaccg cattcgactg
     1141 atacgtcttc cacgagcgca cgtgatcctt gtagagcatc cagatcgtgc tgatggtcag
     1201 cacgacgccg gacaccgcaa agatgtggtg cagcagcttc gtgttgcgcc aggtggattc
     1261 ggtggcgggc atgggcgagg cggagtgagt ggtgagacgt gagtggtgag tagttttgac
     1321 gaccactcac cactcaccac ccacctactc acctaaaaat taagtaggta ctcgggcatc
     1381 gaaatgaaat acttcaaatt gatcgtccag cggccgagca tcttgatcgg cagcagcatc
     1441 atgaacagca ataggttggc catcaccatg tagcggatga agcccatgcg gatgaacagg
     1501 ttctggaaaa acttgctcag cacgacgagc agcggcggaa ccatcgtgaa atagccgatc
     1561 accaggatga tccccggcga ttcgcgcttc agcgccacga gaatcttggt cagctcgcca
     1621 gcgtcgtcgg cggctttcgg acggctggag cccagcagcg tgacccaaaa gtactcggac
     1681 agatcgacgt tgttcagggc ttccaccttg tgggcgtccc agatttcata cggcccgaaa
     1741 aagttccagt tcggcccgcg gaggaacgtg cccatcacga tcaacgtgac ccagagaacc
     1801 aagaagccga tctgaaacat gatgtacgcg aacttgcgct ggtcgatcgt gtaatagcca
     1861 ttcccctgct tgttgaagtc gatatacggg atggccatca ggccggcgat gatcatgctg
     1921 gggaggacga cgcccgccat ccagggatcg taatagacga gcatttcctg caggcccagg
     1981 aagtaccacg gggccttgga cggattgggg gtctttacgg cgctggccgg ctcttccagc
     2041 ggggcctgca gggcgatggc ccaaaagagc agcaccgccg tgagggctac catgcagatc
     2101 agctcggtat aaaccaggtc cggccagacg agcacctttt cgctgtcgag cttctcgagc
     2161 ggctccagcc cttgccgcag gcggtcgtcg ttttgcacgg cccgataggc ggccagccag
     2221 gtgaaaaagc cgagcaaaaa gaccatcgcc acgatcggca cgttatcggg cttgccgacg
     2281 atgttgaaga aattcgggtc ggtcatcgac atgcccatca ggagcagcgc cacgttgagg
     2341 agcgaccagg cgacccacgg cttgacgagg aagcggcgga gcaggaaggc aatgcccagc
     2401 aagaccagcg tgccgagcgt atagatcgtc gggttcatga cccagttgat ccccgcgcga
     2461 accgcagtgg ggatggagat ccaggccata acgtccggac cgccgctata agcaagcgac
     2521 gcaatcaaca caaagcccag cgacacgagc agccagacga tcgccggagt cacgggaaaa
     2581 tccacgcccg gcaggcgaaa gagcggccgg ctgtcggctc gctgccacag gagatacgcc
     2641 gccacgccag tcatcagcgc caggaagatg tagtacgagc ccaggaagcc cgaaatctgg
     2701 ctgaggaaga cgtcgaggga ttggccgtgc atgttcaaca tccaagaacc aaagaaccaa
     2761 gaaccaacgg ctcaaggtga cattcgcgct ttggttcttt ggttcttcgg ttcttcggtt
     2821 cttgagtcat tcgttagagt ggtccactaa tgccgccgtc cttgcggacg cgccagaagt
     2881 ggatcgccat cagcatggcg gcggccagcg ggatggcgac gcagtgcaaa acgtaaaacc
     2941 ggttgagcgt ttcctcgccc acgaagcgcg cgccgaggag gccgaagcgc gcatcggagg
     3001 cgttggtgat catgtcgatg ccgtcgatcg tcaaaagctg attgccaggg ccttcgtagc
     3061 caaggaacgg cgtcgcccgg gccatgttcg agccgaccgt gatcgcccag atggcgagct
     3121 gatcccacgg cagcaggtag cccgtgaacg aaagcaagag cgtcagcaag agcaaaatga
     3181 cgccgatgac ccagttgaac tcgcgcggcg gcttgtagct gccggtgaga aacacgcggt
     3241 acatgtggag ccagacggtg atgaccatgg cgtgggcccc ccagcggtgc aattcgcgga
     3301 ggatgcccag gctcgtcacg tcgcgcagcg ccaggatgtc gttataggcc cattccagcg
     3361 tgggccggta atagaacatg agcaggacgc cggtgatcgt ctcgacgaga aacaagaaga
     3421 acgtcacacc ccccatgcac cacgtgaagc tcagggcgat gccctgcttt ttgatcgaca
     3481 ccgggtgcag gtgcagaaaa aagttcgtga gcatcaccac gatgcggttc cggcgatcga
     3541 ccggcgcagg gtggcggatg atgctcttcc agatttgcga ttcgcggatg atttcgccga
     3601 gggttgccat cgaggagaaa tttcaagaaa caagaaccaa attccaacga atcaggtagc
     3661 tcgcctcttt ggttcttgga tctttgattc ttggatcttg gtttcggatt gccttgctta
     3721 gacggtcaca aacgaggctg tgtctctcca ctgccccagc tcttgctgga aggtgcggct
     3781 cttatccacg aggagctggc cgtcgtcggc caggctgatc gcgtaccgct ccagcgggcg
     3841 aggggccggg ccttcgaaat tcacgccatc cttgtaaaag ccgctgccgt ggcagggaca
     3901 cttgaacttc tgctccgcct ccagccagtt cggcgtgcac cccaggtgcg tgcagacgct
     3961 cttgagggcg aagatcatcg gctggccgtc gaattcgtag cggacgatcc acacgccgaa
     4021 ctgcggaatg tacttcgttt ccacctggcc gggagcgagc gaatcgggga agccgacttt
     4081 gaacttcgtg gggggctcga tgaggatgtt cgggaacatg aacctcgcca ggcccagcag
     4141 ccacatcagg tgcgtcgccg ccagcgacgt aaaaccgatc gccagcgacg agccgaagag
     4201 cacgccggcg ctggccgcca gaaagttccg gcgggtcgcc gtctcgattg gcgctgccgg
     4261 ggcgggggct ctggcgtagt ccggcttggc tggcatcggg ggaacatcga gcttcttctt
     4321 ggcggcgggc gccgcaggag tggccttggc ggcggcctcg gctttggtga tagggcctgg
     4381 cttggcgccc ttgcgggcgg cggccaggat gctcgcggtg tcttt
//
LOCUS       DASUGB010001150         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_126965, whole genome shotgun sequence.
ACCESSION   DASUGB010001150 DASUGB010000000
VERSION     DASUGB010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126965"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2237
                     /locus_tag="VFV87_21845"
     CDS             <1..2237
                     /locus_tag="VFV87_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106484.1"
                     /translation="WIFITTDGQLATERADGSLDLLQAFAVRSETLEGRNLLKWPKAK
                     VETRTPTGRARMFLPLRPPGEYDVSAELTRLEGNGGAFIGLSIAGQPAILVIDGFPEL
                     GGRTGILPRSLSEISNAPLLRSAAPMLQTGSSTNLVVKVRLQDPTRYVVTVENGSRSE
                     AFNGQIADLAAPTEFRIPMQYGMSFGSLDANIRFADFTLRPVRPGLPPELVAKMNGVN
                     LPGSTSAITPSASIPSSQSSAGPNSVASPSPAPAAARKPLPAGDELTAKIEEARSIYQ
                     EEFKLATKPAQKAALARNIRTAGEGTRSDQTARYVLLDLARKVYIQAAEPANALALAR
                     QLEREFEIPPSDLAAATIEGLNDATLPSDERAALAKAAADMADELISTGNYERAESIA
                     AIAVQSANRQKDTDLKKEILQRRAAVARIAKQYADVKSHLEKLVDQAADPVANLAAGR
                     FHCMYLEDWARGLPQLSAAGDGVFSEPAKLDLDAGADSAKQLAAAEAWLNLAETSKAG
                     DRDDKQAIQRRAKSLLQSAVRGLTGLDKVKAEKRLETLQDVGVGRKPPTASASSTAVQ
                     TASPASSAATAPVVAGELVGRARRDGADAGVVVTYRPGYVLTPEDVNRLTQAIGGGQM
                     QIDLIGILTLTSDQELAILHSGGSSASGVNSLYIGNQKISEVGDDKSKEESKTVSLRR
                     GTHLVRWSLTGGDFGASGLLITPATKPAAGAAPPFTFGADQGMQAAARRLPTRQTLSL
                     GDGN"
BASE COUNT          444 a          802 c          784 g          380 t
ORIGIN      
        1 attggatttt catcacaacc gacggccaac tcgccacgga acgggccgac gggtcgctcg
       61 atctcttgca ggcgtttgcc gtccggtcgg agacattgga aggccgcaat ttgctgaaat
      121 ggccgaaggc gaaggtggaa acgcgcacgc ccaccggccg cgcgcggatg ttccttccgc
      181 tgcgcccacc gggggaatac gacgtatccg ccgagttgac gcgcctggaa ggcaatggcg
      241 gggcgttcat cggcctgtcg atcgccggcc aaccggcgat tctcgtcatc gacggatttc
      301 ccgagctcgg cgggcgaacg ggaatcctgc cgcgcagctt gagtgagatt tccaacgcgc
      361 cccttcttcg cagcgcggcg ccgatgctgc agaccggctc gtcgaccaac ctcgtcgtca
      421 aagtccgcct gcaggacccg acgcgctatg tggtgaccgt cgagaacgga agcaggtcgg
      481 aggcgttcaa cggtcaaatt gccgacctgg cggcccccac cgaattccgc atccccatgc
      541 agtacggcat gtcgttcggc agtctggacg cgaatatccg gttcgctgat ttcacgctgc
      601 gacccgtgcg gccgggcttg ccgccggaat tggtcgccaa gatgaacggt gtgaatcttc
      661 ctggatcgac atcggcgatt actccatcgg cgtcgattcc atcgtctcag tcttcggctg
      721 gccccaactc ggtcgccagc ccttcccctg cgcccgctgc ggcccgcaag ccgctgcctg
      781 cgggggacga gctgacggcg aaaatcgagg aagcccgcag catctaccag gaggagttca
      841 agctggcaac caagccggcc cagaaggcgg cgctggcccg caacatccgc acggcaggcg
      901 agggaacgcg cagcgaccag accgcgcggt acgtgctgct cgacttggcc cgcaaggtct
      961 atatccaggc ggcggaaccg gccaacgccc tggcactggc gcggcaactg gaacgcgaat
     1021 ttgagattcc gcccagcgat ctggccgcgg cgacgattga aggactgaac gatgccaccc
     1081 tccccagcga cgagcgggcc gcgctggcca aagccgccgc cgacatggct gacgagctga
     1141 tttccacggg caattacgag cgggcggagt cgattgccgc aatcgccgtg caatccgcca
     1201 atcgccagaa agacacggac ctcaagaaag aaatcctgca gcggcgcgcg gccgtcgcgc
     1261 ggatcgccaa acagtatgcc gatgtcaaat cgcacctgga aaagttggtc gaccaggccg
     1321 ctgatccggt cgcgaacctg gcggccggcc ggtttcattg catgtacctg gaggactggg
     1381 cccgcggctt gccgcagctt tccgcggcgg gggacggcgt attctccgag ccggcgaaac
     1441 tagatctgga tgcgggagcc gactccgcca agcaactcgc cgcagccgag gcctggctca
     1501 atctggctga aacaagcaaa gccggcgacc gcgacgacaa gcaggcgatc cagcggcggg
     1561 ccaagtcgct gttgcaatcg gccgtccgcg gcctgacagg cctggacaaa gtcaaggcgg
     1621 aaaagcggct ggaaacgctc caggacgtgg gcgtcggccg caagccgcct acggcgtccg
     1681 catcctcgac ggcggtgcaa accgcgtcgc ctgcgtcatc ggcagcgaca gcgcctgtcg
     1741 ttgccggcga gctcgtcgga cgggcgcggc gcgacggcgc cgatgccggc gtggtcgtca
     1801 cgtaccggcc cggttacgta ctgacgccgg aggacgtgaa ccggctgacg caggccatcg
     1861 gcggcgggca gatgcagatc gatctgatcg gcatcctcac gctcacgagc gatcaggagc
     1921 tggcgattct gcacagcgga ggttcctccg cctccggcgt caattcgctt tacatcggca
     1981 atcaaaagat cagcgaagtc ggcgacgaca agagcaaaga ggagagcaag acggtctctc
     2041 tccgccgcgg cacgcacctt gtccgctggt cgctcaccgg cggtgatttc ggcgcgagcg
     2101 gtctgctcat cacgccggca acaaagcccg ccgccggtgc agcgcccccc ttcacctttg
     2161 gcgcggatca aggcatgcaa gctgccgccc gccggctgcc cacgcggcaa acgctttcgc
     2221 tgggcgatgg caactaagcg ctattcgtcc ggcaggctga cgtccgtgtc gccgaccgac
     2281 gccgtcgtga cggcctgcgc agttaccagt tccgcttcga tgatcggctg gaattgttcc
     2341 aggcgcggct cgctggccag gacttgctgc caggcggcga tctggctggc cgagcgctcg
     2401 ccggtgcgtt
//
LOCUS       DASUGB010001151         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_684085, whole genome shotgun sequence.
ACCESSION   DASUGB010001151 DASUGB010000000
VERSION     DASUGB010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684085"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFV87_21850"
     CDS             complement(<1..746)
                     /locus_tag="VFV87_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106485.1"
                     /translation="MPEILRPRAARIPRETAVHIHLLGQVDYEDCLSLQRRLAYDALT
                     RADGRIVVLICEHAPLITIGRGGSRANVRFTGAELAERALEIRYVGRGGGAILHAPGQ
                     LAIYPIVPIDWHGWTVGHYLRQLHAALGALLAELKIKPTVVEGSFALAGRGGILAALG
                     VGIRQGVTMHGAFLNVTPDLRDFVRVDVTPGRTMTSLLSHRALPTKMTKVRAALVTHL
                     AEALSLERYHLHTGHPLLADLPDMSERRESA"
     gene            882..1379
                     /gene="smpB"
                     /locus_tag="VFV87_21855"
     CDS             882..1379
                     /gene="smpB"
                     /locus_tag="VFV87_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5106486.1"
                     /translation="MAKADKQTASKPDTGQERVVSENRKARHRFEVLDSLECGIQLTG
                     SEVKSLRDGKLSLDEAYARVKDGAVWLVGADIAEYPQATLWNHLPKRPRKLLMHKEEL
                     RKFAAKAHEKGLTLVPLKLYFNPRGIAKVLIGLCRGKKLHDKRETLKKADSQREMDRA
                     MRHKR"
     gene            1449..2129
                     /locus_tag="VFV87_21860"
     CDS             1449..2129
                     /locus_tag="VFV87_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5106487.1"
                     /translation="MLLLRDIKKTYTEPDGSPLPILNILRFEVQSGEQMVLVGKSGSG
                     KTTLLHVIAGITRADSGQIVIDGIDLAGLSEAGVDKVRAAKIGYVFQTFNLLPGFSAF
                     ENVLLGMTFARGRKDPARARQLLDRVGLAHRLTHKPTALSVGEQQRVAVARALANRPS
                     LLLADEPTANIDPRNQQKIVDLIRDTCREEGVSLVIVTHSPEVAGQFQRVDKLEEINL
                     VDGKAAVN"
     gene            2161..2559
                     /locus_tag="VFV87_21865"
     CDS             2161..2559
                     /locus_tag="VFV87_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106488.1"
                     /translation="MGKRNRFRDHADPVVQEWGRTYPKFPGVAECARLIRAGKARGSM
                     AEIIVHELAENAAECLPEMIATFRDDPDATVRLYVMMALDIARPPAAVGFLADVLREG
                     DPRFTPYAKWALQGINTRESRRALWDATHP"
     gene            2725..4107
                     /locus_tag="VFV87_21870"
     CDS             2725..4107
                     /locus_tag="VFV87_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5106489.1"
                     /translation="MTTSFGSGPAASAADTIEFPCTGCGRTLKVPASAAGKRASCPQC
                     NTVATIPSGSQPLAGPAGMAGMDGRTAIASTASPQTAPWAMPPQPTQPQPAAPQPTPP
                     QQFSPPQQFTPPQTLPPPSTFQPQHPVPPAPAIQPQVTAGRETPTKQLFDRITREIAK
                     IYVGQDELVLGSLVALFSSGHILIESVPGLGKTLFVRSLGRVLGLKFGRIQFTADLMP
                     SDVTGAPVLDMKTQEFRFRQGPIFTQLLLADEINRAPAKTHAALLEIMQEYRVTVDGK
                     SHPIERPFLVLATQNPIESEGTYNLPEAQLDRFMFKLVANYPHENEEAQILKMHSTQL
                     DLNERLEKELQTITSSEEILKTIHENGQVRVDDRLVDYINKVVRRTREWPQFHMGASP
                     RAGLALMQAARTLAAFSGRDYAVPDDVFQIALPSLRHRVALTAEAEVEGQKIDDLLTE
                     LIRSVEVPRL"
     gene            4104..5528
                     /locus_tag="VFV87_21875"
     CDS             4104..5528
                     /locus_tag="VFV87_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5106490.1"
                     /translation="MTPLVDFLRDHGPLLVRISGLIILGLTAPLVALAWWKRIYPHTP
                     LLILLAVPCVLAFALLVNSSLLPVILIIDIVIPLIGLADLFTLPRKKSFSVERETTRI
                     ASLRQNHRVTLHISNLSQRKRTAWIRDDVPQEFEPEPKEFVLKLGPRSRTTVHYELKA
                     TRRGRFELREINVRVRSLLGLWQRFLVYPQPSSIHVYPDLKQLAEYAVLARTNRLSLM
                     GVRRTRRIGMENDFERLRDYTLDDNYKYIDWRASARRLKLTVKDFQTSQSQRIIFLLD
                     CGRLMTNESTGLSLLDHSLNAALLLSFVALRQGDSVGMIAFADRVLSFVPPRGGMKQM
                     NQLLHASFDRFPELVESRYDEAFLYLNTHCKKRSLVVMVSNIIDEVNAHQIESYMRNL
                     VGRHLPLGVLLRDHQLFGAADHEPTNETELFRAAAAAEILTWRHQVLRDLETHGVLAL
                     DVFPEDMTAPLVNSYLEIKARHLL"
BASE COUNT         1069 a         1948 c         1749 g         1002 t
ORIGIN      
        1 gctgattcac gccgttcgct catgtccggc aaatccgcca gcagcggatg gccggtgtgc
       61 aggtgatagc gctcgaggct cagggcttcg gccaggtgcg tgacgagcgc ggctcggacc
      121 ttggtcattt tagtcggcaa cgcccgatgc gacaggagtg aggtcatggt gcggcccggg
      181 gtcacatcga cgcggacaaa atcccgcagg tccggggtga cattcagaaa ggcgccgtgc
      241 atggtgacac cctgtcgaat gccgacgccc agcgccgcca gaattccgcc gcgcccggcc
      301 agggcaaagc tcccttccac gaccgtcggc ttgatcttca attcggccag caaagcgccc
      361 agcgccgcat gaagctgcct caggtaatgg ccgaccgtcc agccgtgcca gtcgatggga
      421 acgatgggat agatagcgag ctgccccgga gcatgcagaa tcgctccgcc gccgcggcca
      481 acgtagcgga tttccagcgc ccgctcggcc agttcggcgc cggtgaagcg cacgttcgcg
      541 cgggagccgc cccggccgat ggtgatcagc ggagcgtgct cgcagatcag gaccacgatc
      601 cgcccgtcgg cgcgcgttag cgcgtcgtag gccagccgcc gctgtagcga gaggcagtct
      661 tcatagtcca cctggcccaa caaatggata tggacggccg tttcgcgcgg aatccgcgcg
      721 gctcggggtc gcaggatctc tggcatctcg atcgccgggg taggggaata gaggtcggcg
      781 agggggcggg actgtagcag gcggtccacc tgcggccaag agagatgcgg cagccgtttg
      841 cgtgcctttt ttgctgcgtc aatttgccgt acaaatcagg catggcgaaa gcagacaagc
      901 agactgccag caagccggat actggccagg aacgggtggt ttccgagaac cgcaaggccc
      961 ggcatcggtt tgaggtgctc gactcgctgg aatgcggcat ccagctcacg gggagcgagg
     1021 tgaagagcct gcgtgacggc aagctgtcgc tcgacgaggc gtatgcccgc gtgaaggacg
     1081 gggcggtgtg gctcgtgggg gcggatatcg ccgagtatcc gcaggcgacg ctctggaacc
     1141 acctgcccaa gcgcccccgc aagctcctca tgcacaagga ggagctgcga aaattcgccg
     1201 ccaaagcgca cgaaaaaggt ttgactctcg tcccgctcaa gctgtatttc aacccccgcg
     1261 gcatcgccaa ggtgctgatc ggcctctgcc gcggcaagaa gctccacgac aagcgcgaga
     1321 ccctgaaaaa agccgacagc cagcgcgaaa tggaccgggc gatgcgccac aaacgctaac
     1381 gaaccccgcc ttggcggctg ccgtacctcc ctctgactac tgactactga ctactgacaa
     1441 cgaacctgat gctcctcctc cgcgacatca aaaagaccta caccgagccg gacggcagtc
     1501 cgctgccgat cctgaacatt ctgcggttcg aggtgcagtc gggcgagcag atggtgctgg
     1561 tcggcaagag cggcagcggg aagacgaccc tgctgcacgt gatcgccggc atcacccggg
     1621 ccgacagcgg ccagatcgtg atcgacggca tcgacctggc cggcctctcc gaagcgggcg
     1681 tcgataaggt ccgggcggcc aaaatcggct acgtctttca gacgttcaac ctgctgccgg
     1741 gcttctcggc tttcgagaat gttctcttgg ggatgacctt tgcccgcggc cgcaaggacc
     1801 cggcccgcgc caggcaactc ctggatcgcg tgggcctggc ccaccgcctg acgcacaagc
     1861 cgacggccct ctcggtcggc gagcagcagc gggtcgccgt cgcccgggcc ctggccaatc
     1921 gccccagcct ccttttggcc gacgagccca ccgccaacat cgacccgcgc aaccagcaga
     1981 agatcgtcga cctcatccgc gacacctgcc gcgaagaagg cgtctcgctc gtcatcgtca
     2041 cccactctcc cgaagtcgcc ggccagttcc agcgcgtcga taagctggag gagatcaacc
     2101 tggtggatgg caaagcggca gtgaattgaa tggcactgta aaggcgacct cgagcgcgac
     2161 atgggaaaac gcaaccggtt ccgcgatcat gcggatcccg tcgttcagga atgggggcgg
     2221 acctatccga agtttccggg cgtggcggaa tgtgctcggc tcattcgtgc cgggaaagct
     2281 cggggctcta tggctgagat catcgtccat gagctcgccg aaaacgcggc cgagtgtttg
     2341 cccgaaatga tcgcgacatt tcgcgacgac cccgacgcaa ccgtccgatt gtatgtaatg
     2401 atggcgctgg atatcgcccg tccgcccgcg gctgttgggt tccttgcgga cgtgctgcgg
     2461 gaaggcgacc caaggttcac gccctacgcc aagtgggcac tccagggcat caatacccgt
     2521 gagtcgcgcc gcgctctttg ggacgccacg catccgtaat tcgcccgcct catccgtgtt
     2581 cgatccgtgt ttcgtccgtg gctccccgcc ccccacccgc caagccgtaa attgggccgg
     2641 accaccaatt tcggggcggc ctgcacgaaa gtatgatggg gagttcctgt ctccctttcg
     2701 gccgattttc cagccccacg gctcatgacg acttcctttg gttccggacc ggctgcctcg
     2761 gcggcggata cgatcgaatt cccctgcacg ggctgcggac ggacgctgaa ggttcccgcc
     2821 tccgcagccg gcaagcgcgc cagttgcccg cagtgcaaca cagtcgccac gatcccctca
     2881 ggcagccagc cgctggccgg ccctgcggga atggccggaa tggatggccg aacggcaatt
     2941 gcttcgactg cgtcgccgca gaccgcgccc tgggcgatgc cgccacagcc aacgcagccg
     3001 cagccggccg cgcctcagcc cactccgccg cagcaattct cgccccccca acaattcacg
     3061 ccgccacaaa cgctcccgcc cccctcgacc ttccagccgc agcacccggt gccccccgcc
     3121 cccgcaatcc agccgcaagt gaccgccggc cgcgaaacgc cgaccaagca gctcttcgac
     3181 cggatcacgc gcgagatcgc caagatttac gtcggccagg acgagctggt cctgggctcc
     3241 ttggtcgccc tcttctccag cgggcacatc ctgatcgaaa gcgtgccggg cctgggcaag
     3301 acgctcttcg tccgctcgct cggtcgcgtg ctggggctga agttcggccg cattcagttc
     3361 acggccgacc tgatgcccag cgacgtgacg ggcgccccag tcctcgacat gaagacgcag
     3421 gagttccggt tccgccaggg gccgatcttc acgcagcttt tgctggcgga cgaaatcaac
     3481 cgcgccccgg ccaaaacgca cgcggccctg ctcgagatca tgcaggagta ccgcgtcacc
     3541 gtcgatggca agagccatcc gatcgagcgg cccttcctgg tgctcgccac acagaatccg
     3601 atcgagtcgg aaggcaccta caacctgccc gaagcccagc tcgaccgctt catgttcaag
     3661 ctcgtcgcga attacccgca cgaaaatgaa gaggcccaaa tcctcaaaat gcacagcacg
     3721 cagctcgacc tcaacgagcg gctggagaag gagctgcaga cgatcacctc gtccgaggag
     3781 atcctcaaaa cgatccacga gaacggccag gtccgcgtcg atgaccggct ggtcgactac
     3841 atcaacaaag tcgtccgccg cacccgcgag tggccacagt tccacatggg ggcttcgccg
     3901 cgggccggcc tggccctgat gcaagccgcc cgcacgctgg ccgcgttctc cggccgcgat
     3961 tacgcggtgc ccgacgacgt cttccagatc gccttgccca gcctgcgcca tcgcgtggcc
     4021 ctcaccgccg aagccgaggt cgaaggccag aagatcgacg atctgcttac cgagctgatc
     4081 cgctcggtgg aggttccgcg gctgtgaccc cgctggtcga ttttctccgc gatcacggcc
     4141 cacttctggt ccgcatttcc gggctgatca tcctcggcct gacggcgccg ctggtggcgt
     4201 tggcctggtg gaagcggatc tatccgcaca cgccgctgct catcttgctg gccgtgccct
     4261 gcgtcctggc gttcgccctg ctggtcaatt ccagcctgct gccggtgata ttgattatcg
     4321 atattgtgat tccgctgatc ggtctggcgg acctttttac gctgccgcgc aagaagtcgt
     4381 tcagcgtgga gcgcgagacg acccgcatcg cttcgctgcg gcagaaccac cgcgtgacgc
     4441 tgcacatcag caacctcagc cagcgcaagc ggaccgcctg gattcgcgac gacgtgccgc
     4501 aggaattcga gcccgagccc aaggaattcg tgctgaaact tggcccccgc agccgtacga
     4561 cggtccacta cgagctgaaa gccacccgcc gcggccggtt cgagctgcgg gagatcaacg
     4621 tccgcgtgcg gagcctgctt gggctgtggc agcggttcct ggtctatccg cagccctctt
     4681 cgatccacgt ctatcccgac ctcaagcagc tcgccgagta cgcagttctc gcccgcacca
     4741 accgcctgag cctgatgggc gtccgccgca cgcggcggat cggcatggag aacgacttcg
     4801 agcggctgcg ggattacacc ctcgacgaca actacaagta catcgattgg cgagcctcgg
     4861 cccggcggct gaagctcacg gtgaaggatt tccagaccag ccaaagccag cggatcatct
     4921 tcctgctcga ttgcggccgg ctgatgacca acgaatcgac cggcctgagc ctgctcgatc
     4981 attcgctcaa cgcggccctg ctgttgagct tcgtggccct gcggcagggg gattcggtcg
     5041 gcatgattgc ctttgccgac cgcgtgctgt cattcgtccc gccgcgcggc ggcatgaagc
     5101 aaatgaacca gcttttgcat gcttcgttcg atcgcttccc ggagctggtc gaatcgcggt
     5161 acgacgaggc cttcctctat ctcaacacgc actgcaagaa gcggtcgctg gtcgtaatgg
     5221 tcagcaacat tatcgacgag gtgaacgccc accagatcga aagctatatg cggaacctcg
     5281 tcggccggca cttgccgctg ggcgtgctgc ttcgcgacca tcagctcttc ggcgccgccg
     5341 accacgaacc gaccaacgaa actgagctgt tccgcgccgc cgccgctgcc gagatcctca
     5401 cctggcggca ccaggttctc cgggacctgg aaacccacgg cgtcctggcc ctcgacgtct
     5461 tccccgaaga catgaccgcc ccactggtga atagttatct ggagatcaag gcaaggcacc
     5521 tgctttaaat ccttctcgtg ctcctgctct tgctcctaat cctcttccta atcttaatcc
     5581 tcctcttgct ccttctcctc cgcctgccgg cccggagcaa gagaaagagc aagaggatga
     5641 ttagcccgga ttattcatgt tgttgagttc tcgtatggtg cggtagttgg aatttgggcg
     5701 gatggggcag gaggcggtga aggaggcgga gggccccctt acccccagag cggttgtcag
     5761 cttgggag
//
LOCUS       DASUGB010001152         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_823549, whole genome shotgun sequence.
ACCESSION   DASUGB010001152 DASUGB010000000
VERSION     DASUGB010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823549"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..982
                     /locus_tag="VFV87_21880"
     CDS             230..982
                     /locus_tag="VFV87_21880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5106491.1"
                     /translation="MDTNYDQIAEQYKRSKLQPWRTHIERYTLLALAGDVSGQAVIDL
                     ACGEGYYTRELRRLGAARVVGVDLSREMIALAQAEEAREPLGIEYRVGDARDVDLPGQ
                     FDLVFAAYLLNYAHTAAELAQMCRAVARSLKPGGRFVTINNHPADPPANFATCKPYGF
                     AKRLEGDLVEGAPIVWKFFLPDGAIELTNYYLSEQTMNETFRAAGLRDVRWHAPKVSP
                     EGLREFGSEYWAAFLACPPVAFITATYDGATP"
     gene            complement(961..1830)
                     /locus_tag="VFV87_21885"
     CDS             complement(961..1830)
                     /locus_tag="VFV87_21885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5106492.1"
                     /translation="MHPDAVSLLPKIDVTRRGFVVTSLASGFALAVRPVTAETITTSA
                     EGLDAGEVKIPVKDGEIPAYRALPAKGGPFPVVLVVQEIFGVHEHIKDICRRFAKLGY
                     LAVAPELYARQGDVSKETDIQKIISTVVSKVPDAQVLSDLDAAALWAKKTGQGDTSKL
                     AITGFCWGGRIVWLYAAHNPDLKAGVAWYGRLTGMTNDLQPKYPLDLAGDLKAPVLGL
                     YGGADTGIPTESVEKMQKALKDAGSKSEIVLYPDTPHGFHADYRPSYRPEAAADGWKR
                     LQAWFKDHGVAPS"
     gene            complement(1949..2335)
                     /locus_tag="VFV87_21890"
     CDS             complement(1949..2335)
                     /locus_tag="VFV87_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106493.1"
                     /translation="MKRWILAIVTVLAIGSIGAQTASAQNYGFLGGGYGYGGADCGYG
                     GYGGYGGYGYSNSGFTPYDPYRVHSAGIGFYGSGIGLSVGFGQPAYYHDTSHLHYHPG
                     GYVRHRGHFDYIPGHYDVHRSGHWHR"
BASE COUNT          498 a          838 c          874 g          478 t
ORIGIN      
        1 atccacctgc cccacaacca gtttgagaaa gatcaaacac cgcgcagcgg cgaaccctcg
       61 cgcaccatgc gccgcaatgt attaaacgca ccggggtgga cacgtctggt ccaatttttt
      121 caagcaaaaa aaagatttct cagatttttc ttgccgcccg gttccttgcc gtagctcgct
      181 gggtacgctt ggtgggcaga atggcacgct cgctggcaat ggacaactga tggacaccaa
      241 ctacgaccag atcgcggagc aatacaagcg gtcgaagctc cagccgtggc gaacgcatat
      301 cgagcggtac acgctactgg cgctggcagg cgatgtatcg ggacaggccg tgatcgatct
      361 ggcctgcggc gaggggtatt acacgcggga actgcgccgg ctgggggccg cgcgggtcgt
      421 cggcgtcgat ctatcccgcg agatgatcgc cctggcccag gcggaagaag cccgcgagcc
      481 gctgggaatc gagtaccgcg tcggcgacgc ccgcgacgtg gacctgccgg ggcaattcga
      541 tctcgtcttc gccgcctacc tgctcaacta tgcccacacc gccgcggaac tcgcccagat
      601 gtgccgcgcc gtggcccgtt cgctgaaacc aggagggcgc ttcgtcacca tcaacaacca
      661 cccggccgat ccgcccgcca acttcgcgac ctgcaagccg tatggatttg cgaagcggct
      721 ggagggggac cttgtcgagg gcgcgcccat cgtctggaag ttctttttgc ccgacggcgc
      781 gatcgagcta accaactact atctcagcga gcagacgatg aacgagacct tccgcgccgc
      841 cggcctgcgc gacgtgcgct ggcacgcgcc caaggtgtct cccgaaggtc tgcgcgagtt
      901 cggctccgaa tactgggccg ccttcctcgc ctgcccgccc gtcgccttca ttaccgcgac
      961 ctatgatggc gccacgccgt gatccttgaa ccatgcctgc agccgcttcc agccgtcggc
     1021 ggcggcttcg gggcgatagc tgggccggta atcggcgtgg aagccgtggg gcgtatcggg
     1081 atagaggacg atttcgctct tggagccagc gtctttgagg gccttctgca tcttctcgac
     1141 ggattcggta ggaatgcccg tgtcggcgcc gccgtagagg ccgaggacgg gggctttcag
     1201 gtcgccggcc agatcgagcg gatacttcgg ctgcaggtcg ttggtcatgc ccgtcagccg
     1261 gccgtaccag gcgacgccgg ctttcaggtc gggattatga gcggcataga gccacacgat
     1321 tcgcccgccc cagcagaagc cggtgatggc gagcttgctg gtgtcgcctt ggcctgtttt
     1381 cttcgcccag agggccgcgg catcgaggtc gctcagcact tgcgcgtcgg gaactttgct
     1441 gacgacggtg gaaataatct tctggatgtc ggtttccttc gagacatcgc cttgccgagc
     1501 atacaattcc ggcgcgaccg ccaggtagcc gagttttgcg aagcggcggc agatgtcctt
     1561 gatgtgctcg tggacgccga agatttcctg cacgacgagc accaccggaa acggcccgcc
     1621 cttggccggc agcgcgcggt aggccggaat ctcaccgtcc ttgaccggaa tcttcacttc
     1681 gcccgcgtcc agcccttcgg cggaagtggt gatcgtctcc gcagtcaccg gccgcacggc
     1741 cagggcgaag cccgaggcga gcgacgtgac gacaaagccg cggcgcgtaa catcgatttt
     1801 cggcagaagg ctgacggcat cgggatgcat ggcgggcgct cctggaaggc ggctgatgat
     1861 gggtgtatct cagccgcgat tctaggacgc cacgggacgg gttggaaacc cgtcctacgt
     1921 gttggcccgc accgccgcag ttcaggcgct accgatgcca gtgcccggag cgatgaacat
     1981 cgtagtgccc ggggatgtaa tcgaagtggc cgcggtgacg cacgtatcca cccgggtgat
     2041 agtgcaggtg gctcgtgtcg tggtaatagg ccggctggcc aaagccgacg ctcagcccga
     2101 tgccgctgcc gtaaaagccg atgcccgcgc tgtgtacacg gtagggatcg tacggcgtga
     2161 agccggagtt gctgtagcca tagccaccgt agccgccata accaccatag ccgcagtccg
     2221 cgccgccata tccatagcca ccgccgagga agccgtagtt ctgagcggag gcggtctgag
     2281 ccccgatcga gccgatcgcg agcacggtaa caattgccag aatccatcgc ttcatgagaa
     2341 tgcactccct gtcaattggc tgggtccggt tcacggcttt gctctgaggg aagcaaacag
     2401 ccgtggtccg atcgtcagcc gatcaaggca gcaggcgtgc cagagcgccc catcgctagg
     2461 gtttatcagc gtgggaagca cattttgacg atctggccca gtacacccga ctcggaagcg
     2521 cactcaaaac gatggcgcac cgtggcgcgg atgacacgct cttttgcgac gaatgccgac
     2581 ttaaggagcg aatggatcgg gctccgcagc cggcgcgggg gccggcttgg caggtgcggc
     2641 gggagtggcg aagggatcgg cggcgggggc ggcgaaggga tcgacacc
//
LOCUS       DASUGB010001153         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1796673, whole genome shotgun sequence.
ACCESSION   DASUGB010001153 DASUGB010000000
VERSION     DASUGB010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796673"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFV87_21895"
     CDS             <1..876
                     /locus_tag="VFV87_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5106494.1"
                     /translation="AEDFGTQGELPSHPELLDWLAVDFREGTGDRGQGTGWDVKRLVR
                     QIVTSATYRQSSAVTPQLVAHDPENRLLARGPRFRLQAEFIRDQALFIAGLYDGRLGG
                     ASVSPYQPAGLWEELMSRQDGANWTAQKYDQSHGIDLYRRTMYTFWKRTCPPPTLSTF
                     DAPDRETCTVRRSRTNTPLQALVLLNDPTYIEAARKFAERILREGGLSTDERLAFAFR
                     TVLSRPPNSAELAVLRTVFEKQLARFRELPDAANSLVRIGEAPADPQFDQAELAAWAM
                     VASVILNTDEAVTKG"
     gene            889..1656
                     /locus_tag="VFV87_21900"
     CDS             889..1656
                     /locus_tag="VFV87_21900"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5106495.1"
                     /translation="MSTRTLQSKQPAPPAIPLLRAGDRLSQREFHRRYEAYPDDRKFE
                     LIGGIVYMMAPAGFEHGRSGYDLTGVLWAYERATPGVVGALGPTVILGASSEPEPDLV
                     LLIAPDHGGRTRLKQINRKHYIEGPPELIVEVAHSSLTLDLNAKRKDYQRGGVHEYIV
                     ICLPEPSVRWFDLQQDAELPLGENGVLRSSAFPGLWIDTPALLDRNLDRVMKTLQRGI
                     ASREHQEFVARLAAQHKRHLSSQRRTSRPKSGKRGKK"
     gene            1653..>2133
                     /locus_tag="VFV87_21905"
     CDS             1653..>2133
                     /locus_tag="VFV87_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106496.1"
                     /translation="MISPAQPAALLTRRHFFGRTATGIGTAALASLLNPGLFAGDVAA
                     IPRGGVRPGVLEKLHFAPKAKRVIYLVMSGGPSHIDLFDYKPKLKELNATELPASVRM
                     GQRITGMTAGQKSFPCAASIFNFEQHGKSGAWVSELLPHIGSIADDIAIVKTLNTEAI
                     "
BASE COUNT          408 a          663 c          688 g          374 t
ORIGIN      
        1 gccgaggatt tcggcaccca gggcgaactg ccgagccatc cggagctgct ggattggctg
       61 gcggtcgatt tccgggaggg gacaggcgac aggggacagg ggacaggttg ggacgtcaag
      121 cggctggtgc gacagatcgt gacttcagcg acgtatcgcc aatcgtcggc tgttacgccg
      181 cagcttgtgg cccacgaccc ggagaatcgc ctgctggccc gcgggccgcg gttccgcctg
      241 caggcggagt tcattcgcga tcaggcgctg ttcattgccg gcttgtacga cggccgcctt
      301 ggcggtgcga gcgtttcgcc ctatcagccg gcgggtttgt gggaagagct gatgtcgcgt
      361 caggacggag cgaattggac agcccagaag tacgatcaaa gccacggaat cgacttgtac
      421 cgtcggacga tgtacacgtt ctggaagcgg acttgcccgc cgccgacgtt gtcaacgttc
      481 gacgctcccg atcgcgagac gtgtaccgtc cgccgcagca ggacgaatac gccgctgcag
      541 gccctcgtgc tgctgaacga cccgacctat atcgaagccg cgcgtaaatt cgccgagcga
      601 attcttcgcg aagggggcct atcgaccgat gagcggctgg cgttcgcatt tcgcacggtc
      661 ctttcccgcc cgccgaattc cgccgaactg gccgttctgc gaactgtctt cgagaagcag
      721 cttgcccgct tccgcgagct gccagacgcc gccaacagcc tcgtcaggat cggcgaagca
      781 cccgccgatc cgcagtttga ccaggccgag ctcgccgcct gggcgatggt ggccagcgtg
      841 attctgaata cggatgaggc agtcacgaag ggctagacta cagacgccat gtcaaccaga
      901 actctccaat cgaagcagcc ggcaccgccg gcgataccgc tgctccgcgc gggcgatcgc
      961 ctttcacaac gggagtttca ccgccgctat gaggcctatc ccgatgacag gaagttcgag
     1021 ctcatcggag ggatcgtcta tatgatggcc ccggctggat tcgaacacgg tcgcagcggg
     1081 tacgacctga cgggcgttct ctgggcctac gagcgcgcta ctcccggtgt ggtaggcgca
     1141 ttaggcccca cggtgattct tggggcgtcg agcgagccgg agcccgatct ggtgcttttg
     1201 atcgcgccgg accacggcgg tcgaactcgt ttgaagcaaa tcaatcgcaa gcactacatc
     1261 gaaggcccgc ctgagctgat cgtggaagtg gcgcacagca gcctgacgct cgacttgaat
     1321 gccaagcgga aggactacca gcgcggcggc gtgcacgaat acatcgtgat ttgcctcccg
     1381 gaaccgtcgg tcaggtggtt cgacctgcag caggacgcgg agctcccgct cggggaaaac
     1441 ggggttctac gcagtagtgc gtttccgggg ttatggattg acactccggc actacttgac
     1501 cgaaatctcg atcgcgtcat gaagacgctc cagcgcggca tcgcatcgcg cgagcaccag
     1561 gagttcgtcg cccgcctggc cgcccagcac aagcggcact tgtcgtcgca acgccgcacg
     1621 tcccgaccga aaagcggaaa gcgaggaaag aaatgatctc gcctgcacaa ccagcggctc
     1681 tgctcacccg tcgacatttt ttcggccgaa cggccaccgg catcggcacg gcagcgctgg
     1741 cgtcgctctt gaacccgggc ctgttcgcgg gcgacgtggc ggcgattccc cgcggcggcg
     1801 tgcggccggg cgtgctggaa aagctgcact tcgctccgaa agcgaagcgc gtgatttacc
     1861 tggtcatgtc cggcgggccg agccacatcg acctgttcga ctacaagccg aagctcaaag
     1921 agctgaacgc gacggagctg ccggcttcgg tgcggatggg gcagcggatc accgggatga
     1981 cggcaggaca aaaatcgttc ccctgtgccg cttcgatctt caacttcgag cagcacggca
     2041 aatcgggggc gtgggtcagc gagctgttgc cgcacattgg ctcgatcgcc gacgatattg
     2101 cgatcgtcaa gacgctgaac accgaggcga tca
//
LOCUS       DASUGB010001154         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_963270, whole genome shotgun sequence.
ACCESSION   DASUGB010001154 DASUGB010000000
VERSION     DASUGB010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963270"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFV87_21910"
     CDS             <1..894
                     /locus_tag="VFV87_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106497.1"
                     /translation="LTPHAARYPFMQLGNSDAENSRAWDNLGRLPWYQPVAALHEQAF
                     SLAEHPTDLCLDGKTPQPLIAVRQYGKGEVVYLGFNETWRLRRRYGEKYYRQFWSQLI
                     YRLGMSHALGADKRFVARLDQQQYRAEDKVTLTVEAYDANYEPLGEEALPDRGLTAEL
                     TVPGAGGAQASTFLVPMLRQGVFEARIPVFAVGSYSLRVKDPITGKFDEQRFEVTPLS
                     AERRRGVRDDKLQTDLAQLTGGKTYDLTTVHRLPDDLQLEAVTERHTRNFALWSTPLW
                     FGVVMVLMLGEWLSRKMIRLS"
     gene            891..2780
                     /locus_tag="VFV87_21915"
     CDS             891..2780
                     /locus_tag="VFV87_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106498.1"
                     /translation="MTSRLATLRSQLAGLRRARASARAATAWSALGVAILWALAGLFL
                     LDVIFELPVFQRLVLMVLVAGGVAWAFWRFTRPLLGWKESEIEMALLVERQQEIDSDL
                     VAALQFEGPEAKRWGSPQLESAVIEYVAAVGRGINVFEGFSREQMLRRGGLLAASLAV
                     VLIAAIIAPQHLAVFGNRLLLGSQHYPTSTVIEQIAINEKPVLAGPSIGELPGQSGMR
                     PASLKAAEGRQIRFTITAAGDTSPKTGTVRLQSAGKGRSRTSFELKRQGDSKLYAGEL
                     PRLLEDVNYQVYLGDAWTDPARLSMVPLPIVETQLTPIFPRYAQSGRDSFDPTGRQVA
                     VLEGTSIQLAIECQNKKRLQSAWLTIKAGESQQKYDLAKTDSDGFRWRLAEENSPLAN
                     IHQELRYELQVLDEDNLGLESPIRGTIRIRPDRPPSGVADVVHKVVLPTAQPLVEYRA
                     MDDYGIAGLALVVEVERAASPGRGRESIATTGTGDGPSSPQLTPDPLHAGGGSAVAPD
                     STPSDMTPAAALPVERRRFDVLKRPEPLTGTQLPLSGQFALALSPLGLVKGDRIKIVL
                     EVTDYRGENDAGEPAGLAYQSDPLVLEISDESGVLAAISEADQRSEERLTDIIKRQLG
                     IGESP"
     gene            2777..4840
                     /locus_tag="VFV87_21920"
     CDS             2777..4840
                     /locus_tag="VFV87_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106499.1"
                     /translation="MTVRIADCGLRIADWIRPLGVLAMCFLVAGAAAAADVDSLRSKQ
                     QAQEKARSLATELVSAVLDIQLRQLEENGLKSLPIYRDIASMKGNIGELAKDQMEEIV
                     QLLVEAQEGTQAERLVKFNAARGKIREVVVQLMAERQKLYRRMQIAKLAAQVRELIVL
                     ETKAYNTTKSLPELKQDVREQLALATIEDQADVQKLYFQLVFALEDVSTWGGQIGAGA
                     SDGLRILRAAQVDPELKKAADLLGKAEFPEAAKSQYAVIKGLMALLEKLEQTQGLIDS
                     DREEALRMVREMLKKQQALREKTKQEELTEKSTEPLIEAQTNLQKELGKLAEALTKFP
                     TTEPLLEEAKAASFEATADLFEEKKAEALEDQNKVIGNLAQIEKVLEQGLDLEQSGKS
                     ADELAAEVQKLQEAKKQLETAAAEQAKATEAAKQNPAAVKPQEQKVAEELAKTDAMEL
                     PSAVEAKVNDAQEAVAEAAEAAADTAAEKAEEAMDAIKQAQAEVAAQLADKQRMQKAV
                     EVGELARAAEALERAAAAEREIAKEAGEAAKEKGLEAAQANDLATDQKDVNDVAAKIA
                     EGVKNTAPEAAQTLAQAKPSAEEAGKQLEAAKQNPGEPSKPAAEKAAGEAIKTADKLA
                     EAAKELRKQAGAKAGELAKLADEQLKPVESARAAVEDAAENAPANKAEALESSARPSR
                     KSSRR"
BASE COUNT          995 a         1488 c         1629 g          729 t
ORIGIN      
        1 ctgacgccgc acgcagcccg ttacccgttc atgcagcttg gcaacagcga cgcggagaat
       61 tcgcgggcct gggacaacct gggccgcttg ccctggtatc agcctgtcgc cgctctgcac
      121 gagcaggctt tctcgctggc cgaacatccg accgatctgt gtctcgacgg caagacgccg
      181 cagcctttga tcgccgtccg gcagtacggc aagggcgaag tggtctattt gggcttcaac
      241 gagacgtggc gtctgcggcg gcggtatggc gagaagtatt accgccaatt ctggtcgcag
      301 ctcatttatc gcctcggcat gagccatgcc ctgggggccg ataagcggtt cgtcgcccgg
      361 ctcgaccagc agcaataccg ggcggaagac aaagtgacgc tgacagtcga ggcctacgac
      421 gcgaactacg agccgctggg tgaagaagcc ctgcccgacc gcggcttgac ggcggaattg
      481 accgttcccg gcgccggcgg cgcgcaagcc agcacgttcc tggtgccgat gctccgccaa
      541 ggcgtgttcg aggcccgcat tccggtcttc gccgtcggct cgtacagcct ccgcgtaaag
      601 gacccgatca ccggcaagtt cgacgagcag cggttcgagg tcacgcccct atcggccgag
      661 cgccgccgcg gcgtccgcga cgacaagctg cagaccgacc tcgcccagct caccggcggg
      721 aagacgtacg acctgaccac cgtccaccgc ttgcccgacg acctccagct cgaagccgtc
      781 accgagcgtc acacccgcaa cttcgccctg tggagcacgc cgctctggtt tggcgtggtg
      841 atggtgctga tgctcgggga atggctctcg cgaaagatga tccgcctctc atgaccagtc
      901 gccttgccac actgcgttcg cagcttgccg gcctccgccg ggcaagggcc agtgcgcgcg
      961 cggcgacggc ctggtcggcg ctgggagtgg cgatcttgtg ggccttggcc gggctattcc
     1021 tgctcgacgt gatcttcgag ctgccggtgt ttcagcggct ggtgctgatg gtgctggtcg
     1081 caggcggtgt ggcgtgggcc ttctggcggt ttactcggcc gctgctcggt tggaaggaaa
     1141 gcgaaatcga aatggccctg ctggtcgagc gccagcagga aatcgacagc gatttggtcg
     1201 ctgccttgca gttcgaaggg ccggaggcaa agcgctgggg ctcgccgcag ctcgagtcag
     1261 cggtgatcga gtatgtcgct gccgtcggcc gcgggatcaa cgtgtttgag ggcttctcgc
     1321 gcgagcagat gctgcggcgg ggcgggctgt tggcggccag cctggccgtt gtcctgatcg
     1381 cggcgatcat cgccccgcag cacctggccg tctttggcaa tcggctgctg ctgggatcgc
     1441 agcattatcc gacgagcacg gtgatcgaac agatcgcgat taacgagaag cctgttctgg
     1501 ccggcccttc aatcggcgaa ttgccagggc aaagtggcat gcggcccgct tcgctgaagg
     1561 ccgctgaagg ccggcagatc cgcttcacca tcactgccgc cggcgacacg agccccaaga
     1621 cgggaaccgt gcgcctccag tccgccggca aaggtcgttc gcgaaccagc ttcgagctca
     1681 agcggcaagg cgacagcaag ctctatgcgg gcgagctgcc gcggctgctg gaagacgtga
     1741 actaccaggt ctacctgggc gatgcgtgga ccgatccggc gcgtctttcg atggttccgc
     1801 tgccgatcgt cgagacccag cttacgccaa tcttccctcg ctatgcccag agcggccgcg
     1861 attcattcga tcccaccggc cggcaggtcg ccgtgctcga agggacgtcg atccagctcg
     1921 cgatcgagtg ccagaacaaa aagcggctgc aatcggcctg gctgacgatc aaggccggcg
     1981 agagccagca gaagtacgac ctggcgaaga ccgattccga tggtttccgc tggcgattgg
     2041 cggaggagaa ttcgccgctg gcgaatatcc atcaagagct gcgctatgag ctgcaggtgc
     2101 tggacgaaga caacctgggc ctggagtcgc caattcgcgg cacgatccgc atccgccccg
     2161 accggcctcc cagcggagtg gccgacgtcg tccacaaggt cgtcctgccc accgcccagc
     2221 cgctcgtcga ataccgcgcg atggacgact atggcatcgc cggcctggcg ctggtggtgg
     2281 aggtcgagcg ggcggcttca cctggaaggg gtcgggagtc aattgcgaca accggcactg
     2341 gcgatggacc gtcgtcgccg caattgactc ccgacccctt acacgccggc ggcggctccg
     2401 cggtcgcacc ggatagcacg cccagcgaca tgacccccgc cgcagccctg ccggtcgagc
     2461 gacgccgctt cgacgtgttg aaacgacctg agccgctcac cggaacgcag ttgccccttt
     2521 ccggccaatt tgccctggcc ctttcgcccc tggggcttgt caaaggggac cggatcaaaa
     2581 ttgtgctgga agtgaccgat tatcgcggcg agaatgacgc gggcgagccc gccggactcg
     2641 cctatcagag cgacccgctc gtgctggaaa tctccgacga aagcggcgtg ctggcggcca
     2701 tcagtgaggc cgaccagcgc tccgaagaac gcttaacgga catcatcaaa cgtcagcttg
     2761 ggattggaga atcgccatga ctgttcgcat tgcggattgc ggattgcgga ttgccgattg
     2821 gattcgcccc ttgggcgtgc tggcaatgtg ctttctggtc gccggcgccg ccgctgcggc
     2881 tgacgtagat agccttcgca gcaagcagca ggcgcaggag aaggcccgca gcctggcgac
     2941 cgagctggtg agcgccgtgc tcgatatcca gcttcgccag cttgaagaga acggcctcaa
     3001 aagtctgccg atctatcgcg acatcgcctc gatgaaaggt aacatcggcg agctggccaa
     3061 ggaccagatg gaagagatcg tccaactgct cgtcgaagcg caggaaggca cgcaagccga
     3121 gcggctggtg aaatttaacg ccgcccgcgg caaaattcgc gaagtcgtcg tgcagctcat
     3181 ggcggagcgg cagaagctct atcgccggat gcagatcgcc aagctggccg cccaggttcg
     3241 cgagctgatc gtcctggaga cgaaggcgta caacacgacg aagtcgcttc ctgaattgaa
     3301 gcaggacgtc cgagagcagc tcgccctggc cacgattgaa gatcaggccg acgtgcaaaa
     3361 gctctatttc cagctcgtct tcgccctgga ggatgtgagc acgtggggcg ggcagatcgg
     3421 cgccggcgcc agcgacggct tgcggattct ccgcgccgcg caagtcgatc cggagttgaa
     3481 aaaagcggcc gatctcctcg gcaaggccga gttccccgaa gccgccaaga gccagtacgc
     3541 ggtgatcaag gggctgatgg cgctcttgga aaagctggag cagacgcagg gattgatcga
     3601 cagcgaccgc gaggaggccc tgcggatggt ccgcgagatg ctcaagaaac agcaggccct
     3661 gcgggagaag acgaagcaag aagagctgac cgaaaaatcg accgagccgc tcatcgaagc
     3721 acagacgaat ctgcagaaag agctaggcaa gctggccgaa gcgctgacga agttcccgac
     3781 gaccgaaccg ctcctcgaag aggccaaggc cgcctcgttc gaggcgacgg cggatctgtt
     3841 tgaggaaaag aaggcagagg cactggaaga tcagaacaag gtgatcggca atctggcgca
     3901 gatcgaaaag gttctggagc aaggcctcga tctggagcaa tcgggcaaga gcgccgacga
     3961 attggccgcc gaagtgcaga agctgcagga agcgaaaaaa cagctcgaaa cagcggctgc
     4021 cgagcaggct aaggcgacgg aggcggccaa gcagaaccca gccgcggtga agccgcagga
     4081 gcaaaaggtc gcggaagagc tggccaagac ggacgccatg gaattgccga gcgctgtcga
     4141 ggcgaaggtc aacgatgccc aggaagcggt cgctgaagcg gcggaagctg ccgcagatac
     4201 ggctgccgag aaggcggaag aagccatgga cgcgatcaag caggcgcagg ccgaagtggc
     4261 cgcgcagctc gccgacaagc agcggatgca gaaggcggtc gaagtgggtg agttggcccg
     4321 cgccgcggaa gccctggaac gggccgccgc tgccgagcgt gaaatcgcga aggaagccgg
     4381 cgaggcagcc aaggaaaagg gcttggaagc cgctcaggcg aacgatctgg caacggacca
     4441 gaaggacgtg aatgacgtcg ccgccaagat cgccgaaggc gtgaagaaca cggctccgga
     4501 agcggcccag acgctcgccc aggcgaagcc gtcggctgag gaggccggca agcagcttga
     4561 ggcggccaag cagaatcctg gcgagccgag caagcccgcc gcggaaaaag cagcaggcga
     4621 agcgatcaag actgccgaca agctggccga agcggccaag gagcttcgca agcaagccgg
     4681 cgcgaaggcc ggggaattag cgaagctggc cgacgagcag ctcaagccag tcgaatccgc
     4741 gcgggccgcc gtcgaagacg cggcggagaa cgctcccgcg aacaaggccg aggcgctcga
     4801 aagctcggcg aggcccagca gaaagtccag caggcgatga c
//
LOCUS       DASUGB010001155         5747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1240022, whole genome shotgun sequence.
ACCESSION   DASUGB010001155 DASUGB010000000
VERSION     DASUGB010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240022"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFV87_21925"
     CDS             complement(<1..510)
                     /locus_tag="VFV87_21925"
                     /inference="COORDINATES: protein motif:HMM:NF037031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEX5106500.1"
                     /translation="MARGAALVALVLAANAARGEITVTTEFEGGSAKVLAIDQEKKMV
                     RIMPAGDPERGWPCWWYFRVDGLKVGDKLSLELVPSTAKLVQPGPRKGLPLDGSWAVP
                     FRATFSTDAKTWRHTDPGRLQFGYAIYNVPVEAESLWLAWGPPFTPQDAAKLVKEIDA
                     AGDWAESFEL"
     gene            complement(977..2668)
                     /locus_tag="VFV87_21930"
     CDS             complement(977..2668)
                     /locus_tag="VFV87_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106501.1"
                     /translation="MPTSPRKPRRWYRWITQFSLRTLLIVVTLSAVVCWWFLQPKTQY
                     EQLSGERLKLRRQVRPVTQADKAVNPFAGELVNAGMWRLYDQFDGLIADGRYEQDQPH
                     GKWTLYHPNGRKAAEGVMTRGLRTGLWKVWNADGQRVSEVTYAIGVATPRVPPESRPW
                     GCYSLVPVVGMVNFLAPTGPVAWQGFGGGGFGSGMMGGGSFGPGMVQPTTLYRWRYTR
                     QPESIRHGPCRAWHATGQLWFEGQYESDLRTGLWKYYDEQGRLIESGTFESDRREGAW
                     EVAGADGVLHAVEYFADRPRDEFERQLAMIRDDLASGAVRRELAAVSRLEELGRHGVP
                     LLVEMLQSKSHRLKFLAVRTLARQSAVPDEAIPKIAKLIDDPDSRLALRAKLVVYLAD
                     PAQRPQLLTPLLEATKAEDDELAFEALAAIYRAGGSRRELVLKALLERMGREPEKYYF
                     YGLYSNPIAQFGDLGEEVLAQLDRIFPALTPDARLAAVFVLQHIVLLRGEPQNIELPS
                     GVIEQHWEIPAAAQPLLQQAKADPDPRVQEAAALVGQATGGFGSHGFAGPAGGFF"
     gene            complement(2692..5283)
                     /locus_tag="VFV87_21935"
     CDS             complement(2692..5283)
                     /locus_tag="VFV87_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106502.1"
                     /translation="MPPKLLFYSLVFLALCVGLPLWSQWQRAVSLRAAAWEQQQADKR
                     LLTELSRPVKAAGQELTLREFVAFLQEQLGQPIVLDEEALDAAAVGLGARRYDVIHLP
                     EGTVTCRSLLASAAEQNDLAFDIDQGRLRITTPERAESPAALLLNVYPIPQPGLTAST
                     TEDEWVELVSTTIQPDTWDEVGGIGYLQSVPGGLAIVQTRDVHDEIRSLFVRLGEMEN
                     PPRSWASVPLSPPEMTAGERRIRQALHQTTSINVMLEPLNDLIANLASKHGFPAILHV
                     RRLGEAGVGAQTPVTLKLKDVTVASVLGAIVEQLELTYIIRNEMVVITTPEEAEKHLP
                     TVAYPVHDLVTFPEVVDFDSLTDLITTTVAPDSWDEVGGPGALTGSGIARGWMIVSQT
                     DKVHREVEALLANIRRALSDGGPGPFPLGPQSAVDLKIRDALEREVAFDYERVAIADV
                     CDELSQSLGINVRLDAKWLEAAGINVETPITCHFPRARVRVQLRRLCDALEMDFLVRS
                     EALWLTTPEEAQSNLITRIYDVRPLIDPDIGITDVDGLQELITSHLQPDQWDEVGGPG
                     SFQIFRGLAVVSQTEETHRQVRDMLLQLENHCLPYSQPAEPSPSVVHISADPIEHRIE
                     AALSQRFDVDLPEAPLGQTLEKLAQSAGIPITCDCPLSGNFQDTPTYALAGRQLRLDS
                     VLDHLLTPLGLGYTIRDQELVVMSKAWSKENLRTRLYRVDDLLKSGKWTIDGFRRWAW
                     RNLEPDYWDEMGGPGVVSVTGLGWLAVTADVTHHRMVEAWHQRERARNGPPTARDFVA
                     IGLSISNGIRRYPEIKLPVELVDVLADHDGSPERVLLVGTSRLATFPLPPKPPVPDPF
                     ADDPFAP"
     gene            5490..>5747
                     /locus_tag="VFV87_21940"
     CDS             5490..>5747
                     /locus_tag="VFV87_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5106503.1"
                     /translation="MSRIVLAMSGGVDSSAAAHLLKEAGHEVIGVFMRHGEEAQEVCA
                     KDGPPGFKLPIFDSRSDHKQGCCSASDAEDARRVADRLQIPF"
BASE COUNT         1055 a         1910 c         1752 g         1030 t
ORIGIN      
        1 cagctcgaag ctctcggccc agtcgcccgc cgcgtcgatc tctttcacca acttggctgc
       61 atcttgcggc gtgaagggcg ggccccacgc gagccacaga ctctccgctt ctaccggcac
      121 gttgtaaatc gcatacccaa actgcagccg gccgggatcg gtgtgccgcc atgtcttggc
      181 gtcggtcgaa aacgtcgccc ggaatggcac cgcccacgaa ccatccagcg gcagcccctt
      241 ccttggccct ggctggacga gcttcgccgt cgaggggacg agttcgagcg agagcttatc
      301 gccgaccttc agcccatcga cccgaaaata ccaccagcag ggccagccgc gctccggatc
      361 gccggcgggc atgatccgga ccatcttttt ctcctgatca atcgccagca ccttggccga
      421 cccgccttcg aattccgttg tgacggtgat ctcaccccgc gccgcattcg ccgccaggac
      481 gagcgccacc agcgcagccc cgcgcgccaa ggcggacagc caacaataag ggcacctgat
      541 acgctcgagc gtgcgcatcg acgagtgctg gcaaatcaat tcgcttcccc aagctagagc
      601 cggtttggat aagaccgcag ccggctcccc tttttccgcc aggctgcggc ccgcattgta
      661 accgcccgct ggaaaaaaat ctcgattttt ttagctgacg cgcaacttcg gactgcgcca
      721 gtttccagcc agtgatgcaa gctcctcggc ccctgcctcg atttacaccg cttaacgcca
      781 aactacgcga tttattgccg cccagcagtg tgctcaacgt cgttccaact ccttgccccg
      841 acattctttc cgccacggcc cgacctgcca cctaccccct cgctttcagg cgcgccagtg
      901 ccgggttcca gtggcgactc tagtggcatg tccaccccga cggatcgagt gcttggcccg
      961 tcatgtctgg gaggcactaa aagaacccgc ccgctggacc ggcgaatccg tgactgccaa
     1021 acccgccggt tgcctggccg actagtgcgg ccgcttcttg cacccgcggg tcggggtcgg
     1081 ctttggcttg ctgcaggagc ggctgggcgg ctgcggggat ctcccagtgc tgctcgatca
     1141 cgccgctggg cagctcgata ttctggggct caccgcgcaa caggacgatg tgctggagca
     1201 caaaaaccgc cgccagccgg gcgtccggcg tgagcgctgg gaagatacga tccaactggg
     1261 ccagaacctc ctcgccgagg tccccgaact gtgcgatggg attggaatac aggccgtaga
     1321 aatagtattt ttccggctcg cggcccattc gctcgagcag ggctttgagg accagttccc
     1381 gccggctccc gcccgcgcga tagatcgccg ccagcgcctc gaaggccaac tcgtcatcct
     1441 cggccttagt cgcttcgagc aatggcgtca gcaactgggg ccgctgggcg gggtccgcca
     1501 gatagaccac aagcttcgcc cgcagggcaa gccgcgaatc gggatcgtcg atcagctttg
     1561 caatcttcgg aatcgcctcg tccggcacgg ccgattgccg ggccagcgtg cggaccgcca
     1621 gaaacttcag gcggtgcgac ttgctctgga gcatttcgac cagcagggga actccgtggc
     1681 ggcccagctc ctccaaacgc gagacggcgg ccaattcgcg gcgtaccgcg ccgctggcca
     1741 ggtcgtcccg aatcatcgcg agctgccgct caaactcgtc tcgcggtcga tcggcaaaat
     1801 actcgaccgc gtgcagcacg ccatcagcgc ccgcgacttc ccaggcccct tcgcggcggt
     1861 cgctctcgaa cgtgccgctc tcaatcaggc gaccttgctc gtcgtagtac ttccacaagc
     1921 cggtgcggag gtcgctctcg tactggcctt cgaaccagag ttggccggtg gcgtgccagg
     1981 cccggcaggg gccgtggcgg atcgattccg gttgccgggt gtatcgccag cggtagaggg
     2041 tggtgggttg aaccatgccg gggccaaagc tcccgccacc catcattccg gagccgaagc
     2101 ccccaccgcc aaagccctgc caggcgaccg ggccggtggg ggctaggaag ttgaccatgc
     2161 cgacaacggg aactagtgaa taacagcccc atggtcgcga ctcgggcggc acgcgcggcg
     2221 tggcaacgcc aatcgcatac gtcacctcgc tcacccgctg gccgtcggcg ttccagacct
     2281 tccacaggcc cgtccgcagg ccgcgcgtca tcaccccttc ggcggctttg cggccattgg
     2341 ggtggtacag cgtccacttg ccatgcggct ggtcttgctc gtagcggcca tcggcgatca
     2401 agccgtcaaa ctggtcgtac aaacgccaca tgccggcatt caccagctcg ccggcgaagg
     2461 gattgaccgc cttgtccgcc tgcgtcacgg gccgcacctg ccgccgcagc ttcagccgct
     2521 cgccggaaag ctgctcatac tgcgttttgg gctgtaggaa ccaccagcag acgacggcgg
     2581 aaagcgtgac cacgatgagc aacgtccgca ggctgaactg cgtgatccag cgataccagc
     2641 ggcgcggctt tcggggcgaa gtgggcataa gatcgtttgg ataattgcct atcagggagc
     2701 aaacggatcg tcggcgaatg ggtctggcac gggcggtttc ggtgggagcg gaaacgttgc
     2761 gagccggctg gtaccgacca gcagcacgcg ctccggcgac ccgtcatgat cggcaagcac
     2821 gtcgaccagc tctacgggca acttgatttc tggataccgt cgaatcccgt tcgatattga
     2881 cagcccgata gcaacaaagt cgcgcgctgt cggcggcccg tttcgtgctc gttcgcgctg
     2941 gtgccacgct tcaaccatcc gatgatgcgt gacatcggct gtgacggcga gccagccgag
     3001 tccggtgacg ctgacgacgc cggggccgcc catttcgtcc caataatctg gttctaagtt
     3061 gcgccaagcc catctgcgaa acccgtcgat cgtccacttg ccgctcttga gcaaatcatc
     3121 aacccggtac agccgcgtcc gcaggttctc cttgctccag gccttgctca tgacgaccaa
     3181 ttcctggtcg cgaatcgtat agcccagccc cagcggcgtg agcagatgat ccaggacgct
     3241 gtccagcctg agctgccgcc cggcgagggc gtacgtcggc gtgtcctgaa aatttcccga
     3301 aagcgggcag tcgcacgtga tcggtatgcc agcactctgg gccagctttt ccagcgtctg
     3361 ccccagcggc gcctccggca gatcgacatc gaatcgctgc gagagggccg cttcgatccg
     3421 gtgttcgatc ggatcagccg agatgtggac gacgctcggc gacggctcgg ctggctggct
     3481 gtacggcagg cagtggtttt caagctgcaa aagcatgtcc cgcacctggc gatgggtttc
     3541 ctcggtctgc gaaacgacgg ccagtccgcg aaagatttgg aaggagccgg ggccgccaac
     3601 ttcgtcccac tggtcgggct gcaagtgcga tgtaatcagc tcctgcaagc cgtccacgtc
     3661 ggtgatcccg atatccggat cgatcagcgg ccgcacgtcg tagatccgcg tgatgagatt
     3721 cgactgggct tcctcggggg tcgtcagcca caaggcttcg ctacggacca ggaaatccat
     3781 ctccagcgca tcgcacaagc ggcgaagctg cacccgcact ctggcccgcg gaaaatggca
     3841 ggtgatgggc gtttcgacgt tgatcccggc cgcttcaagc catttggcat ctaggcggac
     3901 attgattccc agcgactgtg agagctcgtc gcaaacatcg gctattgcca cccgctcgta
     3961 gtcgaacgcg acctcccgct ccagggcgtc ccgaattttc agatcgacag ccgactgcgg
     4021 ccccagcgga aacggccccg gcccaccgtc gctgagagcc cggcgtatgt tggccaggag
     4081 cgcctcgact tcacgatgaa ctttgtcggt ctggctgacg atcatccagc cgcgggcgat
     4141 accgctgcca gttagagcgc ctggacctcc cacctcgtcc caggaatcgg gagcaaccgt
     4201 ggtcgtaatc agatccgtca gcgaatcaaa atcgactact tctggaaacg tcaccaggtc
     4261 gtggacggga taggcgacgg tcggcagatg tttttcggcc tcctccggcg tcgtgataac
     4321 caccatttcg ttgcggatta tgtacgtcag ctctagctgc tcaacgatgg ctcccagcac
     4381 ggacgcgacc gtcacatcct tcagcttcag cgtgaccggc gtctgcgcgc cgacgccggc
     4441 ctctcccagc cggcgaacgt ggagaatggc aggaaaaccg tgtttcgacg ccagattggc
     4501 gatcaagtcg ttgagcggct ccagcatcac gttgatcgac gtggtttggt gcagagcctg
     4561 gcggatgcgg cgctcgccgg cggtcatttc cgggggcgaa agggggacac tggcccacga
     4621 gcgcggcgga ttttccatct cgcccaggcg gacaaacagc gagcggatct cgtcgtgaac
     4681 gtcgcgggtt tgcacgatgg ccagcccgcc gggaaccgac tgcaggtagc cgatgccgcc
     4741 aacttcgtcc caagtgtccg gctgaatcgt ggtgctgacc agctcgaccc attcgtcctc
     4801 ggttgtggac gcggtcaagc ccggctgcgg gatgggatag acgttgagca agagggcggc
     4861 gggcgactcg gcccgctccg gcgtcgtgat gcgcaggcgg ccttggtcga tgtcgaaggc
     4921 caggtcgttc tgctctgcgg ccgacgccag cagcgagcgg caagtaactg ttccctccgg
     4981 cagatgaatc acatcgtagc gccgcgcgcc tagcccgacc gcggctgcgt ccaaagcttc
     5041 ctcgtcgagc acgatcggct gcccaagctg ctcctggaga aaggcgacaa actcccgcag
     5101 cgtcagctcc tgccccgccg cctttaccgg gcggctcaac tctgtcagca gccgtttgtc
     5161 ggcctgctgc tgctcccaag ccgctgcacg cagagaaacg gcccgctgcc attggctcca
     5221 caggggcagg cccacgcaca gggccagaaa gacaagcgaa tagaagagga gcttcggggg
     5281 catcgccgca cgccctgttt ctttgccctg gtcgcaagga tatgcccacc gagggcgaaa
     5341 ggcaaggctt ttccgaattc agaatgcaga atgcagaatt cagaatgcgg aatgaaaacc
     5401 gatggaccgc tcgccagccc gcgcggttta tcgaagaata aggatgcctg aaattcgcaa
     5461 tccaaaatcc gaaatccgca attcccccca tgtctcgcat cgtcctcgca atgtccggcg
     5521 gcgttgatag cagcgccgcc gcgcacctgc tcaaggaggc agggcacgag gtgatcggcg
     5581 tcttcatgcg gcatggcgag gaggcccagg aggtctgtgc gaaggacggc ccgccggggt
     5641 tcaagctgcc gatcttcgac agccggtccg atcacaagca gggctgctgc tcggccagcg
     5701 acgcggagga cgcccgccgc gtggccgacc ggctccagat tccgttt
//
LOCUS       DASUGB010001156         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1101885, whole genome shotgun sequence.
ACCESSION   DASUGB010001156 DASUGB010000000
VERSION     DASUGB010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101885"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /gene="polA"
                     /locus_tag="VFV87_21945"
     CDS             <1..2006
                     /gene="polA"
                     /locus_tag="VFV87_21945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5106504.1"
                     /translation="VEALAGKFGDRDSGLGVRDEGDREQGTGDGDQEPGVRNRQPENP
                     QSAIRNPQSPPLTTADSPEWQANYQTIATPEDLARLVGLLAEQKQISIDTETTSIYPR
                     FAEIVGYSLAWAPGEACYIPVRASAADPQLDPAAVAAALKPVLENPEIAKIGQNLKYD
                     LIVLRNIGIELRGLAFDTMVADYLLEPGERSHNMDELARRHLAHQTITIDQLIGSGKS
                     QKRMDEVPVPLIAQYAAEDADVPLRLMGLLAPRLQASGLADLFNDLEMPLIEVLAEME
                     FNGIRVDLARLRELSGEFNDRIMRLEREITELAGREFNIDSRNQLSQLLFEELKLPVL
                     KRTKTGPSTDAEVLEELALLHPLPAKIIEYRHNAKLKSTYVDALQELIHPVTGRVHTS
                     FKQDVAATGRLSSQDPNLQNIPIRTEIGRAIRSAFLPGPPGWLLLTADYSQIELRVLA
                     HFSGDATLRQAFADDVDIHAQVASEVYGVPLDQVTSTQRRNAKAVNFGVIYGQSPFGL
                     AKALGIEQREAADFIERYFARYPGVDRFILETLQQCRRDGYVSTILGRRRPVSGVRDP
                     EKLRDKRQRTLPERIAVNAVIQGSAADIIKRAMIQVHRRLHVENFQAKLLLQIHDELV
                     FEFPPEEQERLTQLVTTEMAAAADLTVPLKVDLNTGSTWAACE"
     gene            2281..>3468
                     /locus_tag="VFV87_21950"
     CDS             2281..>3468
                     /locus_tag="VFV87_21950"
                     /inference="COORDINATES: protein motif:HMM:NF024647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEX5106505.1"
                     /translation="MLIVTVSMYLRARAECERLRVELARFREISDLERHASDQQAKAE
                     SAIANVEKARTDLEGIQSQIAAHSTTISSQDAHILAQRREIESAATTLGAYKSLDDLQ
                     KQIEKQKQRVNQYNQVLGNFKSAEALAAHITAQTNRVQELNRTIGRFDTILKMDERIA
                     AQQSQVNTLMARTQDLNEVLGGASTAGELQARINYQQNLLAELQSRAGQVEEVLEMQE
                     FGFYRPRFDFDSSSRYQKMLDMVRDQQTSMVKNNTAVQWEKVWIVEGSDAKGRKMMAE
                     QTKLMLRAFNGECDASVAKVKYNNATTLESRIKRSCDQINKLGKTNSASITGAFLQLK
                     LQELYLAHEFRVKQQQEKEEQQRIREQLKDEEKARREIEEAQEKATRDEEVAERALER
                     ARLA"
BASE COUNT          767 a         1086 c         1047 g          568 t
ORIGIN      
        1 gcgtggaggc gctggcgggg aagtttgggg atcgggattc ggggctcggg gttcgggacg
       61 aaggggacag ggaacagggg acaggggatg gggaccagga gccaggcgtc aggaatcgac
      121 agccagagaa tccgcaatcc gcaatccgca atccgcaatc gcccccgctc accaccgccg
      181 attcgcccga gtggcaggcc aactaccaga ccattgccac tcctgaggat ctggcccggc
      241 tggtcggctt gctcgccgag caaaaacaga tctcgatcga taccgaaacg acctccatct
      301 atccacggtt cgcggaaatc gtcggctatt cgctggcctg ggcgccgggc gaggcgtgct
      361 acatccctgt gcgggcgtct gcagccgatc cgcagctcga tccggccgcc gtcgcagccg
      421 cattgaagcc cgtcctggaa aaccccgaaa tcgccaagat cggccagaac ctcaagtacg
      481 acctgatcgt gctgcggaat atcggcatcg aactccgcgg cctggcgttc gacacgatgg
      541 tcgccgatta cctgctggaa ccgggcgagc ggagtcacaa catggatgag ctggcccgcc
      601 gccacctcgc ccatcagacg atcaccatcg accagctcat cggcagcggc aaaagccaga
      661 agcggatgga cgaagtcccc gtgccgctca tcgcgcaata cgccgccgaa gacgccgacg
      721 tccccctgcg gctgatgggc ctgctggccc cgcggctgca ggccagcggc ctggccgatc
      781 tgtttaacga cctggaaatg ccgctgatcg aagtgctcgc tgagatggag ttcaacggca
      841 tccgcgtcga tctggcccgg ctccgcgaac tgtcgggcga gttcaacgac cgcatcatgc
      901 ggctggagcg cgaaatcacc gagttggccg gccgcgagtt caatatcgac tctcgaaacc
      961 agctcagcca gctcctcttc gaggaactca agctccccgt gctcaagcgg acgaagaccg
     1021 gccccagcac cgacgccgaa gtgctcgagg agctcgccct tctgcacccg ctgccggcga
     1081 agatcatcga gtaccgccac aacgccaagc tcaagagcac gtacgtcgat gccctgcagg
     1141 aactgatcca ccccgtcacc ggccgcgtcc acacgtcgtt caagcaggac gtggccgcca
     1201 ccgggcggct cagctcgcag gatcccaacc tgcaaaacat cccgatccgc accgaaatcg
     1261 gccgcgcgat ccgctcggcc tttctgccag ggccgcccgg ctggctgctc ctgacggccg
     1321 actattcgca gatcgagctg cgcgtgctgg cccatttttc gggcgacgca acgctgcggc
     1381 aggcctttgc cgacgacgtc gacattcacg cccaggtcgc cagcgaggtc tatggcgtgc
     1441 cgctagacca ggtgacgagc acgcagcgcc gcaacgccaa ggcggtgaac ttcggcgtca
     1501 tttatggcca gtcgcccttc ggcctggcca aggcgctggg gatcgagcag cgcgaagcag
     1561 ccgacttcat cgaacgctac ttcgcccgct atccgggggt cgatcggttc atcctggaga
     1621 cgctccagca gtgccgccgc gatggctacg tgagcacgat cctcggccgg cggcggccgg
     1681 taagcggcgt tcgcgatccg gaaaagctcc gcgacaagcg gcagcggacc cttcccgagc
     1741 ggatcgccgt caacgccgtc atccagggct ccgccgccga catcatcaag cgggcgatga
     1801 tccaggtcca ccgccgtctg cacgtcgaaa acttccaggc caagctcctg ctgcaaatcc
     1861 atgacgaact ggtcttcgag ttcccgcccg aagagcaaga gcgcctcaca cagctcgtca
     1921 ccaccgaaat ggccgccgcc gccgacctga ccgtcccgct caaggtcgat ctcaacaccg
     1981 gctccacctg ggcggcgtgt gagtagctcg cggccgctcc cgacaggcga gtgcctgtgt
     2041 tttcgccgcg aaaacacagg gtggtttgcg ggtgaagcag gctcgccagc ggaattctcc
     2101 gccgccggga ttccgtttgc gtgtcacccc ctcgatgaag tacaaggggt tgataacgcc
     2161 catgtgggtt agccatgggt ggtcgtctgg cgaacgattc tccttcgaca tcgggaacta
     2221 acggcgatga tttctgcaat cgcaatcgct tgccttgtgc tcagctgttt attggtcgta
     2281 atgctcattg tcaccgtgag catgtatctt cgggcgaggg ccgaatgcga acgcctgcga
     2341 gtcgaacttg ccagatttcg cgagatttcc gacctggaaa ggcatgcgag cgaccagcag
     2401 gcgaaagcgg agtccgcgat cgccaacgtt gagaaagctc gcaccgatct cgaaggtatt
     2461 cagtctcaaa tcgccgctca cagtaccaca atctcgtcgc aggacgctca cattctcgcc
     2521 cagcggcgcg aaatcgaatc ggcggcgacg acgctcggcg cctacaagag tctggacgac
     2581 ctccagaaac agatcgagaa gcagaagcag cgcgtcaatc aatacaacca ggtgttgggc
     2641 aacttcaaat ccgccgaggc gctggccgca cacatcaccg cccaaacgaa tcgggttcaa
     2701 gagctgaatc ggaccatcgg aaggtttgac acgattttga agatggatga gcgcattgcg
     2761 gctcagcagt cgcaagtcaa cacgctgatg gcgagaactc aagacttgaa cgaagtgctc
     2821 ggcggcgcga gcacggcggg agagttgcag gcgcggatca attaccagca gaacttacta
     2881 gccgagcttc aatcccgcgc tggccaagtt gaagaggttc tggaaatgca ggagtttggg
     2941 ttctatcgtc cgcgcttcga cttcgactcg tcatcgaggt accagaagat gctggacatg
     3001 gtccgggacc agcaaacatc tatggtcaag aacaacacag ctgttcaatg ggaaaaggtt
     3061 tggatcgttg aagggagcga cgcgaaaggt cgcaagatga tggccgagca gacgaaactg
     3121 atgctgagag cgttcaacgg cgagtgcgac gcttccgttg cgaaggtgaa gtacaacaac
     3181 gcgacgactc tcgagagccg gatcaaacga tcgtgcgacc aaatcaacaa gctcggaaag
     3241 acgaactccg ccagcatcac gggcgccttt ctgcagttga agcttcagga gctttacctc
     3301 gcgcatgaat tccgagtcaa gcagcagcag gagaaggaag aacagcaacg aatcagagag
     3361 caattgaagg acgaggaaaa ggcccggcgg gaaatcgagg aggctcaaga gaaggcaacc
     3421 agagacgagg aagtcgctga acgggctctc gaacgcgcgc gcctcgca
//
LOCUS       DASUGB010001157         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2074746, whole genome shotgun sequence.
ACCESSION   DASUGB010001157 DASUGB010000000
VERSION     DASUGB010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074746"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1127)
                     /gene="galE"
                     /locus_tag="VFV87_21955"
     CDS             complement(144..1127)
                     /gene="galE"
                     /locus_tag="VFV87_21955"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX5106506.1"
                     /translation="MRILVTGGAGYVGSHCVRRLLQAGHEPLVYDNLSRGHAAAVPAE
                     RLVVGDLSEQHKLAGLLRERKIEAVMHFAALALVGESVEQPSLYYANNVVGSFHLLEA
                     MRLAGVTKIVFSSTTATYGAPEQMPIAETTPQQPINPYGFSKLVVERMLDDYAAAFGF
                     GAAALRYFNAAGAAADGSVGEDHTPESHLIPIVLQVALGQRERISLFGDDYPTPDGTC
                     VRDYVHVEDLADAHLAALSRLQAGEVLKANLGTGRGYSVREVVEACRRISGHAIPESI
                     APRRPGDPPELVADARLAREKLAWQPRHTTIETIVETAWNWHRTHPHGYGG"
     gene            1254..3065
                     /locus_tag="VFV87_21960"
     CDS             1254..3065
                     /locus_tag="VFV87_21960"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX5106507.1"
                     /translation="MSQPESVDLDRRRRVVGWRIWWAEWLLTVLTALALVLGHGVERW
                     ETELAVELGALAVVSMTLLTAGLVWRLTLSRDWVEFLRRRQLLVIVHAGWLLLLPVMM
                     LFGLPPMLRFGSGISDVLIWSELAFWMRVVASGVHVVRYIAAARGNPAFVFVGSFALL
                     ITVGTLLLMLPACRSQPAGGQRETGAPPLVALFTATSASCVTGLIVVDTPTYWSRTGQ
                     TVILALIQLGGLGVMTFGAFFALGQRRGFLVREGVFMGQLLEADDRQAVRRMIASILI
                     FTLASEAVGAALLWTLLPGETPAESGWFGIFHSISAFCNAGFSLYSDNLEGKELRWQV
                     FGVIAPLIIVGGLGFDVLRNATQVAFSRITDRWFRGNGRHSSAPPRLTVTSRLVLVTT
                     LGLLVAGAVAFYVLETGEILRGQSLSQRLANSWFQSVTFRTAGFNMVDFSKTTPSTRL
                     VAVGMMFIGASPGSTGGGIKTVAFALMVLATAATVRGRDRLEIAGRTIPDEFVRRGAA
                     VVAMALAVLMASTLLIVMFEQRPDLFLDHLFETTSAQGTVGLSSLGTHRLRPASQLVL
                     VATMFIGRVGPLTLLVALARRRAEPKYSYPTERVMLG"
     gene            3236..3829
                     /locus_tag="VFV87_21965"
     CDS             3236..3829
                     /locus_tag="VFV87_21965"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5106508.1"
                     /translation="MVRSRAWISLLILAPVAAAAIFTRPYLAFEGLPEYSIEVLAWVL
                     FFCGVAVRWWATLFIGGRKDAALVTQGPYSVCRNPLYLGTLLITLSVGVFLQSLSLLL
                     ATALVGAGYVLITVPVEERKLAERYGRSFDEYRQRVPCLLPNFSLLTEPAEIRVIPCG
                     IHAEFKRSIQYAAIPLICYLIEHLRMMPSWPTFFTLP"
BASE COUNT          577 a         1286 c         1295 g          833 t
ORIGIN      
        1 ggtgcgatcc tgttctgggt cgatacggtt gcccccatgc ccgcgcattg gaagtggggc
       61 gaggcgccct atctgctgct aaccggcgct ctggtgctcg ccctgcagcg tctctcctgt
      121 cgatcgccca agagcgagaa cgcctacccg ccgtagccgt gcgggtgcgt gcgatgccaa
      181 ttccaggcgg tttcgacgat tgtctcgatc gtcgtatggc gcggctgcca ggcgagcttc
      241 tcgcgggcca ggcgcgcgtc ggcaacgagc tcgggtggat cgccgggacg acgcggggcg
      301 atcgactctg gaattgcgtg gcccgaaatg cggcggcaag cctcgacgac ctcgcgcacg
      361 ctgtagccgc ggccggttcc gaggttcgct ttcagcacct cgcccgcttg caagcggctc
      421 agggcggcga ggtgcgcatc ggccaggtcc tcgacgtgga cgtaatcgcg gacgcatgtg
      481 ccgtcaggcg tcgggtaatc atctccgaag aggctgatcc gctcgcgctg ccccagggcg
      541 acctgcagta cgatgggaat caagtgcgat tcgggcgtgt gatcctcgcc cacgctgcca
      601 tcggcggctg ctcccgcggc gttgaaatac cgcagcgcgg ccgcgccaaa gccgaatgca
      661 gccgcatagt cgtccagcat ccgctccaca accagcttgc tgaagccgta gggattgatc
      721 ggctgctggg gcgtggtttc cgcgatcggc atttgctctg gtgcgccgta ggtggcggtt
      781 gtgctggaaa aaacgatctt cgtgacgccc gccagccgca tcgcttccag taaatgaaac
      841 gagccgacga cgttgttcgc gtagtagagg cttggctgct cgaccgactc gcctacgagc
      901 gccagggcgg caaaatgcat caccgcttcg atcttccgct cgcgaagcaa tccggcgagc
      961 ttgtgctgct ccgataaatc acccaccacc agccgctcag ccgggaccgc ggcggcatgc
     1021 ccgcgggaga gattgtcata gaccagcggc tcgtgcccgg cctggagcag ccggcggacg
     1081 cagtgcgagc cgacgtaacc tgcgcctccg gtgacgagaa ttctcatggc gaccatcttg
     1141 gcagaatttg gcccgctagt ccaaccgtgt gccgggcgcg ttctgccgtt gcgttcgtgg
     1201 ggcctctggt gacaatgagc ggacttcgtt tagcccttgc aaggccgccg cccatgtctc
     1261 aaccggagag tgtcgatctg gatcgccggc ggcgcgtcgt gggttggcga atctggtggg
     1321 ccgagtggct gctgacggtg ctgaccgccc tggcgctggt gctggggcac ggcgtcgagc
     1381 ggtgggaaac cgagctggcc gtcgagctgg gtgcgctggc cgtcgtctcc atgacgctgc
     1441 tcacggcagg gctcgtctgg cggttgacgc tgagccgcga ttgggtcgaa tttctccgca
     1501 gacgtcagct tctggtaatc gttcatgccg ggtggttgct gctgttgccg gtgatgatgc
     1561 tcttcggcct ccctccgatg ctgcgcttcg gcagcggaat cagcgacgtc ctcatctggt
     1621 cggagctggc gttctggatg cgtgtcgtcg ccagcggcgt ccacgttgtc cgctacatcg
     1681 ccgcggcccg cggcaacccg gcatttgtgt tcgtcggctc atttgctttg ctcatcaccg
     1741 tcggcacgct cttgctcatg ctccccgcct gccgcagcca gccggccggc ggccagcgcg
     1801 aaaccggcgc gccgccgctg gtcgcgctct tcacggccac cagcgcgagc tgcgtcacgg
     1861 ggctgatcgt cgtcgatacg ccgacttact ggagccgcac cggtcagacc gtcatcctcg
     1921 ccctgattca gcttggcggc ctgggtgtga tgaccttcgg agccttcttc gcgctgggtc
     1981 agcgccgcgg ctttctcgtc cgggaggggg tcttcatggg ccagctcctc gaagccgatg
     2041 accgccaggc ggtccgccgg atgatcgcct cgattctgat cttcacgctc gccagcgaag
     2101 cggtcggcgc ggctctgctg tggaccctgt tgcccggcga aactccggca gaaagcggct
     2161 ggttcggcat ctttcattcg atcagcgcgt tttgcaacgc gggcttttcg ctgtattccg
     2221 ataatctcga ggggaaggag ctgcgctggc aagtgtttgg cgtcatcgcg cccttgatca
     2281 tcgtcggcgg actcggattc gacgttctgc gcaacgccac ccaggtcgcc ttcagccgca
     2341 tcacggatcg ctggtttcga ggaaacggcc gacattcgtc ggccccgccg cggctgaccg
     2401 tcacgtcgcg cctggtgctg gtgacgacgc tcggtctgct cgtggcagga gccgtggcgt
     2461 tctacgtcct ggaaacgggc gaaatcctcc gcggccagtc gctctcgcag cggctggcca
     2521 actcctggtt tcagtcggtc acgttccgca ccgccggctt caacatggtc gatttttcga
     2581 agacgacccc ttcgacgcgg ctggtggcgg tcggcatgat gttcattggg gcctcgccgg
     2641 gctcgaccgg cggcggcatc aaaacggtgg ccttcgccct gatggtgctg gccaccgccg
     2701 caaccgttcg cgggcgggac cggctggaaa ttgccgggcg gacgatcccg gacgaatttg
     2761 tccgccgcgg cgccgcggtc gtcgcgatgg ctctggccgt gctgatggcc tcgaccctct
     2821 tgatcgtcat gttcgagcag cggccggacc tgttcttgga tcacctgttc gagacgacca
     2881 gcgcccaggg aaccgtgggc ttgtctagcc tcggcacgca ccgcctgcgg cccgcctcgc
     2941 agctcgtcct cgtcgccacg atgttcatcg gccgcgtcgg cccgcttacg ctgctcgtcg
     3001 ccctcgcccg ccgccgcgcc gagccgaagt attcgtaccc caccgagcgc gtgatgctgg
     3061 gttaagcggc ttgggccagt catttttgcc tgttacgcgg tcagcaggca gagtcgaatg
     3121 aaatgtccct ttacagttca gctcccgacc gcaatattca tgagtatcag tattccgctt
     3181 ggaccatcat tgttcggcat cgatgccagc gaagtctttt tccgtacgcg cttttttggt
     3241 ccggtcgcgc gcgtggattt cgctcctgat cctggcgccg gttgccgccg cggccatctt
     3301 cacgcgcccc tacctcgcct ttgagggctt gccggaatac agcatcgagg tattggcctg
     3361 ggtgctgttt ttctgcggtg tagccgtgcg ctggtgggca acactgttca tcggcgggcg
     3421 caaggatgcg gccctggtga cgcaaggccc gtactcggtt tgccgcaatc cgctctactt
     3481 gggaacgctg ctgattacct tgtcggtggg cgttttcttg caaagcttgt cgctcctgtt
     3541 ggctactgct ctggtggggg ccggctacgt gctcatcacc gtgccggtcg aggagcgcaa
     3601 gctcgccgag cggtacggcc ggagcttcga cgagtatcgc cagcgcgtcc cctgcctgct
     3661 gccaaacttt tcgctgctca cggagccggc cgaaattcgg gtgattccgt gcggcattca
     3721 cgccgagttc aagcgctcga ttcagtacgc ggccatcccg ctgatctgct acctgatcga
     3781 gcacctgcgc atgatgccgt cctggccgac gttttttaca ttgccgtaga gccacgtccg
     3841 gctgaagccg gcgtcttgcc gcgtcaatcc ggctgaagca ggctgcgaga acgtgcagca
     3901 gcatgtacgc aagcgtagaa tctgaatcgg gtcgcgtctt acaaccagga ttgaggctct
     3961 tgccgcgtga cgaagaagtg tggggtgctc c
//
LOCUS       DASUGB010001158         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_127935, whole genome shotgun sequence.
ACCESSION   DASUGB010001158 DASUGB010000000
VERSION     DASUGB010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127935"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(563..1312)
                     /locus_tag="VFV87_21970"
     CDS             complement(563..1312)
                     /locus_tag="VFV87_21970"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX5106509.1"
                     /translation="MMVALRIWAWVGAAALAASVAVSPAHAALMTYGFTNVTNNSAAD
                     VATGIAQMTVGVSDVGVASNQVKFIFSNSGPNASSITDVYFDDGTLLSVAYVIDGEDD
                     VAYAGTKYAEGASPGELPGGSTINWNDEGGHHFSADSDPPAQPNGANPGESFAVVFDL
                     LGGKTYADVIAAIALSLQPANIGTDVEGGLRIGIHVQGFAGDGSESFVNTTPPVLAPV
                     VPEPGSMAMWGLGLALCGTVGYFRRLQKFAA"
     gene            complement(1494..2120)
                     /locus_tag="VFV87_21975"
     CDS             complement(1494..2120)
                     /locus_tag="VFV87_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106510.1"
                     /translation="MPTSENPARKAPRRQTAHKTEPLSPAEADGRLVQKCLAGDSAAW
                     SQLYQEFHDRLLASIRPILGTLASDATLVDEIGARVWYALVKNDFELLGRFDAARGCQ
                     LSTFLGIIAKREAKQLLRSERRRRNREYGASRPDVCSGSAADLDAAAEQEFLATLTPS
                     ERTFYSDVLVGPPVVGAVRNYSQSNVWQLTHRVREKLSQFLFEGDTQS"
     gene            complement(2360..>3441)
                     /locus_tag="VFV87_21980"
     CDS             complement(2360..>3441)
                     /locus_tag="VFV87_21980"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5106511.1"
                     /translation="AVFTVTLTGTIESDVSVDFATVAGTASADEDYTPAAGTLTFIPG
                     GPSTQQIHVPIVNDSLSEATEKFTVQLTNSSADTLITKSVGSGTILDDDVSLSIGDAG
                     AREGTPKGKKGGSTQYTSLTFNVVLSAPASAEVRVHYATQNGTAAAGSDFVATGGDLV
                     FSKGEISMPITVNVLMDSDAEKDEQFQVVLSAPVGASISDGVGDGTIVDDDTKTRGKP
                     PKGGQIAKEFELDALVPELDVFAFGPAAADHHEQSIAAAASGSGDLVSNSLAAGLLVG
                     DFACHSDLTVKPLAIASAATSLDAPPHYLQRLVETNKRELASENSEVNKTSRTRATVS
                     DEEWLELLAQDLVSRWSGQPDDQTG"
BASE COUNT          704 a          999 c         1022 g          716 t
ORIGIN      
        1 tgacgcggaa tagattgtgt ggccaaggtc ggatgccggt cacggttctc gcgctcagac
       61 ccattgcggt ctggctgcga gtgcttgcgg ccagtagctc gctgcttctt ggcgggcgac
      121 gagactggcg cggcgctagc gcgtgtaggc gcggacatgc gaaaggcgga cgactagcga
      181 ctcaacaaac gagacgagag agagcactag gtgggagatg cgttcagccg ttgatatcgg
      241 ctcattgctc tgcgcggact tcgtccccgt gggatgcgct cgtgttctct gttctgcggt
      301 tccggcagcc gttctcttct ttgacgctca ggcaatgctg agcagtcagg gagactaaac
      361 gactaaggtg cctaaggtaa ttgaatcgca agacttgtca aggagccgga agtggcggac
      421 gaacagattt tacgattgtg aaggcgcgaa tgcggtgaca gccaaagggc ggactgttaa
      481 tccacggata acgccgccaa aagcacaagg ggctgcatat tgcagcccct tgaacgtggt
      541 ttcattgaat tacaatcgcg gcctaggccg caaacttctg caggcgacgg aagtagccga
      601 ccgtgccaca cagggccagc ccgaggcccc acatcgccat cgaaccgggc tcgggcacga
      661 cgggtgcaag tacagggggc gtcgtattga caaacgattc agacccgtca ccagcgaagc
      721 cttgcacgtg gatgccaatt ctcaagccac cttccacatc tgtgccgatg tttgctggct
      781 gcaggctgag ggcaatcgct gcaattacat ccgcatacgt ctttccaccc agcaggtcga
      841 acaccacggc aaaactttcg ccgggattag cgccattcgg ttgagctggt ggatcgctat
      901 cggcactgaa gtgatgcccg ccttcgtcat tccagttaat cgtactgccc cccggaagtt
      961 cccctgggct tgccccttcc gcgtacttcg taccagcata agcaacgtca tcttctccgt
     1021 cgataacgta tgcgacactg agcaatgtgc cgtcgtcaaa ataaacgtcg gtaattgaag
     1081 acgcgttcgg gccggagttg gagaaaatga acttcacttg attcgacgca acgcccacgt
     1141 cggagacgcc gaccgtcatc tgagcaatgc ccgtcgcaac atcagccgcg ctgttattgg
     1201 tgacgtttgt aaatccatac gtcatcagtg cggcgtgagc aggcgacaca gccacactcg
     1261 cggcaagggc ggcagctcca acccaggccc aaattcgtaa agcaaccatc atggaaggat
     1321 cctctttttg ctttccgact gcctcgcgtc cgtattgggt gaaacgagga acgccggatt
     1381 ggtttctacg ggttgtttgc cgccaagctg attgtcctga cacgcacctc cagaaaatcc
     1441 gaaaatcaga tccaactcgc aaccccttac cgcggaagaa gttacagaaa tcgtcacgac
     1501 tgggtatcgc cttcaaagag gaactgggat agtttttccc gcacgcgatg cgtcagttgc
     1561 cagacgttgg attggctata gttgcgaacg gctcccacaa caggcgggcc cacgagcacg
     1621 tcgctgtaaa acgttctctc gcttggagtt agggtggcca ggaactcctg ctcggctgcc
     1681 gcatccaggt cggccgcgga gcccgagcac acgtccgggc gagaggcgcc atattcccgg
     1741 tttcgccgtc gccgctccga gcgcaaaagc tgcttggctt cgcgtttggc aatgattccc
     1801 aaaaacgtcg aaagctggca gccacgggcg gcatcgaacc gtcccaggag ctcgaaatcg
     1861 ttcttcacca gggcatacca gacccgggcg ccgatttcgt ccaccagagt ggcgtcgctg
     1921 gccaaagttc ccaaaattgg acgaatcgaa gccaagagcc ggtcgtgaaa ctcctggtag
     1981 agctgcgacc aggccgccga atccccagcc aggcatttct gaaccaggcg gccgtcagcc
     2041 tcggctgggg atagcggctc tgtcttgtgc gccgtctgac ggcggggagc cttgcgagcc
     2101 ggattttcag aagttggcaa gctcgatttc aaggatgaaa tcgcaattct caggcgccag
     2161 agaggctaac cggagcgaat aaatactgcc gtttcaattg gcaaaccagc gcggacttcg
     2221 tccccgtggg gtgcgccgca cgtcttttcg gcgcgggagg cgaatcaacg tggatgagtc
     2281 taatcgcggt gctgccggtg tgcaagcagc gggaagcttc cgccgccgag atttttcgat
     2341 cgggcactga ttcgccgcat cagcccgtct ggtcgtccgg ttggcccgac caccgtgaga
     2401 cgagatcttg cgccagcaat tcaagccatt cctcgtcgct gaccgttgca cgggtccggc
     2461 tcgtcttgtt gacctcgctg ttctcagagg ccaattctct cttgttggtt tccacgagcc
     2521 tctgcagata atgcggcgga gcgtccaaac tggttgcggc tgacgctatg gcgagtggtt
     2581 tgaccgtcaa atcgctgtgg caggcaaaat cgcccacgag gaggcccgca gcgagtgagt
     2641 tcgaaaccag gtcgcctgat ccagatgcgg cggcggcgat ggattgctcg tgatggtcgg
     2701 cagcggctgg cccaaaggca aagacgtcca gctcaggcac gagcgcgtcg agttcgaact
     2761 ccttggcaat ttggccgccc ttcggtggct tgccccgagt cttggtgtcg tcatcgacaa
     2821 tggttccatc gcctacgccg tcggaaatgc tggccccgac tggcgcgctc agcacgacct
     2881 ggaattgctc gtccttttcc gcgtcggaat ccatcaggac gttcaccgtg atcggcatgg
     2941 aaatctcgcc cttcgaaaag acgagatcgc caccggttgc aacgaagtcg ctgccggccg
     3001 ctgccgtgcc attttgcgtt gcatagtgga ctcgcacctc cgcactggct ggcgcggaga
     3061 gcacgacatt gaaggtcagc gacgtgtatt gtgtgctgcc tcctttcttg cccttgggcg
     3121 tgccttcgcg ggcgccggcg tcgccaatgg agagcgaaac atcgtcgtcc agaatcgttc
     3181 cgctcccgac cgacttcgtg atcaacgtgt cggccgaaga gttggtcagt tgcacggtga
     3241 atttttcggt ggcttccgac aagctgtcgt taacgatggg gacgtgaatt tgctgggtcg
     3301 atggtccacc gggaataaac gtcagcgtgc ctgctgccgg cgtgtaatct tcatcggccg
     3361 aggctgttcc ggcgacggtt gcaaaatcga cggaaacgtc tgattcgatc gtgccggtga
     3421 gcgtcacagt gaacaccgcc t
//
LOCUS       DASUGB010001159         6962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1102289, whole genome shotgun sequence.
ACCESSION   DASUGB010001159 DASUGB010000000
VERSION     DASUGB010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102289"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1685
                     /locus_tag="VFV87_21985"
     CDS             108..1685
                     /locus_tag="VFV87_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5106512.1"
                     /translation="MPRPFYTNAASRPIGRRSFLWACGSLAAAAIWSSRALGAVIQNP
                     KFADYPFQLGVASGDPSPSGFVIWTRLAPQPMEGGGLAAEEIEVAWQVAEDEQMTKVV
                     RSGKAIASPDWAHAVHVEVEGLQPDRWYWYQFRAGGEVSPKGRSRTFPAAGAQPEKLR
                     LAFASCQHYESGYFTAYEHMAAEDIDVVAHLGDYIYEGAASKGKVREHNSREIETLDE
                     YRNRHALYKTDPALQAAHAAFPWLLTWDDHEFDNNCAGAISEEAGILPAAFLERRARA
                     YQAYYEHMPLRRACLPKGPDMQLFRQLDFGNLVNFFVLDTRQYRTDQPCGDGNKPQCD
                     AALDPQATLLGDAQEKWLTAGLASSAAKWNLLAQQVMMARVDRIPGEMVAYSMDQWPG
                     YEMNRRRLLKFFHERRISNPVVIAGDIHSNWANDLIADFDHLDSQVVASEFVGTSISS
                     GGDGARTPKDLDRLLAENPFVRFHNAERGYVRCTVTPERWQSDYQVVEYVTKKGAPLV
                     TRASFVVEDGQPGVKPA"
     gene            1712..4645
                     /locus_tag="VFV87_21990"
     CDS             1712..4645
                     /locus_tag="VFV87_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5106513.1"
                     /translation="MQRSLSNVWLFGALAAGLLASIAPSLRGEDTAADFAREVQPIFA
                     AHCFKCHGPAKQLGGLRLDERGAALAGGDSGAVIVAGKASASQLIERITSTDESIRMP
                     AESEPLSAQQIETLTRWIESGAAWPQASGEAKPLHWSLVPIQRHESKAASGSAIDAFI
                     AAKLREKNLSPAPPADRRTLIRRLYFDLIGLPPTPEEAAEFVADGDPQAYEKLVEKLL
                     TSPRHGERWAQHWLDVVRFAESDGFETNKPRANAWPYRDYVIEGFNRDKPFDQFVREQ
                     LAGDALGADAATGFLVGGANDVVKSPDPVLTAQQQADVLHDIVSTTGSAFLGLTVGCA
                     RCHNHKFDPIPQADYYALAAVFAGVAHGERPVTTQQNRERPELLAALRARLDDLDAQL
                     LRYEPLADVTSDPAILAAEKRLRPAVHPRLNVERFAPVVAKRVRMTVHKTNLYEPCID
                     ELEVLAAGDSPRNVALANSGAKPAASSVYPNSTIHRLEHINDGQLGNGRSWISNEQGG
                     GWIELEFPQSVEIEAVRWGRDREEKYKDRLAIEYTLEVAGEDGAWTAVATHRDREPFL
                     AGQPFDPAAGLAHLPPQVRATADALLAERAKVEAEIKDLTAQPMLYAGVFTKDPAATH
                     RFHRGDPMQPREAIQPGGLRAIEVAYDRVGFAHQDPANDSVAGAAVGAALPTEDQRRR
                     LALAEWIVDPANPLTARVIVNRLWQHHFGEGIVATPSDFGVNGARPSHPELLDWLAAE
                     LIDSGWSLRHIHRLIVTSATYRQSSNVEFGMRNAESEPGTRSIPHSALPTPHLIDADN
                     RLLWRYSSRRLEAEPLRDTILAISGKLDLTMGGPGFLAFEPNDNYVRVYNPKQAFGPT
                     DWRRMIYMTKIRMHQDGTFGAFDCPDGGQIAPRRASSTTPLQALNLLNGPFLVQQAEL
                     FAERVRREAGPTAEAQVRRAFLLAYQREPADEELAASIEIVKAHGLPVLCRALLNSNE
                     FLFVF"
     gene            4657..6138
                     /locus_tag="VFV87_21995"
     CDS             4657..6138
                     /locus_tag="VFV87_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106514.1"
                     /translation="MDLPPLTPAGRQLLDRRSFLADSAAGLGAVALAALLADERKTSA
                     AEPLRPQWSPEKPYAPRLPHFAPKAQQALVIFCSGALSHLDTWDYKPELIARHDQPLP
                     GTGEKFITFQGENGNVTRSPWEFKPRGESGKMISDLLPNLAELADDLCFVHSMTAKSN
                     THGPAENQMSTGFTLDGFPSMGSWVSYALGSESQDLPAFVAIPDPRGVPQIGPNHWNS
                     AFLPAAFQGTAFNADKPIPNLARPQQFGPAAEKATRDLLKLLNERHLAEHPGDSQLAA
                     RIASYELAARMQLGAAEVGNLAEESEATHRLYGTGEANDLKARFARNCLLARRLLERG
                     VRFVQLFNGAYAMGEGVGNWDGHRKLHEQYQVHAPILDQPCAALIRDLKSRGLLGHTV
                     VLFVTEFGRMPTFQKGASGRDHNPHGFTAWLTGAGVKRGFSYGATDEFGHKAVENVAT
                     IYDLHATLLHLLGLEHERLSFYHNGIERRLTDVHGHVLTDILL"
     gene            6146..>6962
                     /locus_tag="VFV87_22000"
     CDS             6146..>6962
                     /locus_tag="VFV87_22000"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEX5106515.1"
                     /translation="MNMRSTWIGTVLLLAAWLPAAVCAADGDSFAEIERDFAARAQPL
                     VKTFCLDCHSAAAKEGELDLERFRSLADVRRGTTVWLKVAEMLDNREMPPKDAERQLT
                     GEQRQQLRGWIERFLHAEALATAGDPGPVVLRRLNSAEYTYTLRDLTGVDSLDPAREF
                     PADGAAGEGFTNTGNALVMSPALITKYLDAGKEVASHAVLLPDGFRFSPATTRRDRTN
                     ETLARIREFYAQFTDAGGGEKVNLQGVVIDTNEGGRLPLEAYLAATLAEREAIA"
BASE COUNT         1241 a         2312 c         2273 g         1136 t
ORIGIN      
        1 tagagtccgg cggcgtgaga gtctatgata gcctccgcgg cggatgttcg cgtcagtccg
       61 ccagatttgc ggtgcatccg caacttgaac gcaggacagg agtttcgatg cctcgaccgt
      121 tctatacgaa tgctgcttcc cggcccatcg gccgtcggtc gttcctgtgg gcgtgcgggt
      181 cgctcgccgc ggcggcgatt tggtcgtcgc gagcgctcgg cgccgtaatc cagaacccga
      241 aattcgccga ctatccgttc cagctcggcg tggcgtcggg cgatccttcg cccagcggct
      301 ttgtgatttg gacgcgcctg gcgccgcagc cgatggaagg cggcggcctg gcggcggagg
      361 agatcgaagt cgcctggcaa gtagccgaag acgagcagat gacgaaggtc gtccgcagcg
      421 ggaaggcgat cgccagtccc gactgggccc acgcggtcca cgtcgaggtc gagggcctgc
      481 agccggaccg ttggtactgg tatcagttcc gcgccggcgg cgaggtcagc cccaaggggc
      541 ggtcgcggac cttcccggcg gccggcgcgc agcccgagaa gctgcggctg gcgtttgcgt
      601 cgtgccagca ctacgaatcg ggctacttca ccgcctacga gcacatggcc gcggaggaca
      661 tcgacgtcgt ggctcacctg ggcgattaca tctacgaggg agccgcgagc aagggcaagg
      721 tccgcgaaca caacagccgc gagattgaga cgctggacga ataccgcaat cgccacgccc
      781 tctacaagac cgaccccgcc ctgcaagcgg cccacgcggc gtttccctgg ctgttgacgt
      841 gggacgatca cgagttcgac aacaactgtg ccggcgcgat ttcggaggaa gcgggcattt
      901 tgccggctgc cttcctcgag cggcgggccc gcgcctatca ggcctattac gaacacatgc
      961 cgctgcggcg ggcatgcctt ccaaagggcc cggacatgca gctctttcgc cagctcgatt
     1021 ttggcaatct agtgaacttc tttgtgctcg acacgcggca gtaccggacc gaccagccct
     1081 gcggcgacgg caacaagccg cagtgcgacg ccgcgctcga tccgcaagcg acgctgctgg
     1141 gcgatgcgca ggaaaaatgg ctgacggcgg gcctggcttc ctcggcggcc aaatggaacc
     1201 tgctcgccca gcaggtgatg atggcccgcg tcgatcgcat ccccggcgaa atggtggcct
     1261 acagcatgga ccagtggccg ggctacgaga tgaaccgccg ccgcttgctg aagttttttc
     1321 acgagcgacg gatttccaat ccggtcgtga ttgcgggcga cattcacagc aactgggcca
     1381 acgacctgat cgccgatttc gaccacctgg actcccaagt cgtcgccagc gagttcgtgg
     1441 gcacgtccat ctcctcgggc ggcgacggcg ctcgcacgcc gaaagacctt gaccgcttgc
     1501 tggccgaaaa tccgttcgtg cggttccaca acgccgagcg cggctacgtc cgctgcacgg
     1561 tcacgcccga gcgctggcag tccgattacc aggtcgtcga atacgtcaca aagaagggcg
     1621 cgccgctcgt cacgcgggcc tccttcgtcg tcgaagacgg ccagccgggc gtcaaaccgg
     1681 cgtaaagtgc gtcgccagtg cgggaatcga catgcagaga agtctgagca acgtttggct
     1741 gttcggggcg ctcgccgccg gcttgctcgc atcgatcgcg ccgagcctgc ggggcgaaga
     1801 cactgcggct gatttcgcac gcgaggtgca gccgatcttc gcggcccatt gcttcaaatg
     1861 tcacggcccg gcgaagcagc ttggcggcct gcggctggat gagcgcggag cggcgctggc
     1921 gggaggcgat tcgggcgcgg tgatcgtggc aggcaaagct tcggccagcc aacttatcga
     1981 gcgcataacc agcaccgacg aatcaatccg catgcccgcc gaaagcgagc cgctttccgc
     2041 acagcagatc gaaacgctga cgcgctggat cgaaagtggg gcagcctggc cgcaggcgtc
     2101 cggcgaggcg aagccgctgc attggtcact cgtgccgatt cagcgacacg agtcgaaggc
     2161 ggccagcggt tcggccatcg acgcgttcat tgccgcgaag ctccgcgaga agaacctgtc
     2221 gcccgcgccg ccggccgatc gccgcacgct cattcgccgg ctgtactttg atctgatcgg
     2281 cttgccgccg acgcccgagg aagcggcgga gttcgtggcc gacggcgacc cgcaggcgta
     2341 cgagaagctc gtggaaaagc tgctcacttc cccgcggcac ggcgagcgct gggcccagca
     2401 ctggctggat gtcgtccgct tcgccgaaag cgacggcttt gagacgaaca agccgcgggc
     2461 gaatgcctgg ccgtatcgcg actacgtgat cgagggtttc aatcgcgaca agccgttcga
     2521 ccagttcgtg cgggagcagc ttgcgggcga cgcgctgggc gcggatgcgg caaccggctt
     2581 cctcgtcggc ggggccaacg acgtggtgaa aagccccgat ccggtcctca ccgcccagca
     2641 gcaggccgac gtgctgcacg acatcgtcag caccacgggc agcgcattcc tcggcctgac
     2701 cgtcggctgt gcccgctgtc acaaccacaa attcgaccct attccgcagg ccgactatta
     2761 cgcgctggca gccgtgttcg cgggggttgc gcacggcgag cggccagtga cgacgcagca
     2821 aaatcgcgag cggcccgagc ttctggccgc cctgcgggcg cgcctcgatg atctcgacgc
     2881 gcagcttctg cgctacgagc cgctggccga cgtgacgagc gatccggcga tactggccgc
     2941 ggagaagcgg ctgcggccgg ccgtccatcc gcggctgaat gtcgagcgtt tcgcgccggt
     3001 tgtcgcgaaa cgtgtgcgga tgaccgttca caaaaccaat ctgtacgagc cgtgcatcga
     3061 cgagttggag gttcttgcgg ctggcgattc gccgcggaac gtcgcgctgg ccaattccgg
     3121 cgcgaagccc gcggcgtcga gcgtttatcc gaactccacg atccatcggc tggagcacat
     3181 caatgacggg cagctcggca acggccgaag ctggatttcc aacgagcagg ggggcggctg
     3241 gatcgaactg gagtttccgc agagcgtgga aatcgaggcc gtccgctggg gccgcgatcg
     3301 cgaggagaag tataaagaca ggctggcgat tgaatacacg ctcgaagtgg ccggcgaaga
     3361 tggcgcgtgg acggcggtcg cgacgcaccg cgaccgcgaa ccgttcctgg ccggccagcc
     3421 gttcgatccc gccgccggcc tcgcccatct gccgccccaa gtgcgagcga cagccgatgc
     3481 cctgctggcc gagcgggcca aggtcgaagc cgaaatcaag gatctcaccg cgcagccgat
     3541 gctgtatgcc ggcgtgttca cgaaggaccc ggcggcgacg caccgctttc atcgcggcga
     3601 tccgatgcag ccgcgcgagg caatccagcc gggcggattg cgggcgattg aggtggcgta
     3661 cgatagggtg ggcttcgccc accaagaccc ggcgaacgac agtgtcgctg gagctgcggt
     3721 gggcgcagcc ctccctacgg aagatcagcg gcggcgactg gcgctggccg agtggatcgt
     3781 cgatccggcc aatccactca ccgcccgcgt gatcgtcaac cggctttggc agcatcactt
     3841 cggcgaaggg atcgtcgcca cgcccagcga tttcggcgtc aacggcgcgc gtccctcaca
     3901 tcccgagttg ctcgattggc tcgctgccga actgatcgac agcggctgga gcctgcggca
     3961 catccaccgg ctgatcgtca cgtcggcaac gtaccggcag agttcgaatg tggaattcgg
     4021 aatgcggaat gcggaatccg agccaggcac ccggtcaatt ccgcattccg cgctccccac
     4081 tccgcatttg atcgacgccg acaaccgcct cctctggcgc tattcctccc gccggctcga
     4141 agccgagccc ttgcgcgaca caatcctcgc catcagcggc aagctcgatc tgacgatggg
     4201 cgggccgggc tttctggcct tcgagccgaa cgacaactac gtccgcgtct ataaccccaa
     4261 gcaggcgttc ggcccgactg attggcggcg gatgatttac atgacgaaga tccgcatgca
     4321 ccaggacggc accttcgggg ccttcgattg ccccgacggc ggccagatcg ccccccgccg
     4381 cgcgagcagc accacgccgc tgcaggcgct caacctgctc aacggcccgt tcctcgtgca
     4441 gcaagccgag ctgttcgccg agcgcgtccg ccgcgaagcc ggccccaccg ctgaagccca
     4501 ggttcgccgc gcgttcttgc tcgcctatca gcgcgagccg gcggacgagg agctggctgc
     4561 cagcatcgaa atcgtaaagg cccacggact tcccgtgctc tgccgggcgc tcctcaacag
     4621 caacgagttt ttgtttgtct tttgaccgga tcgatcatgg atttgccgcc actcacgccc
     4681 gccggccgcc agttgctcga ccgccgcagt tttcttgcgg atagcgccgc gggcctcgga
     4741 gcggtcgcgc tcgcggcgct gcttgccgac gagcggaaga cgagcgcggc cgagccgctg
     4801 cgcccgcaat ggtcgcccga gaagccgtat gccccgcggc tgccgcactt tgctcccaaa
     4861 gcgcagcagg ccctggtcat cttctgctcc ggagcgctaa gccatctcga cacctgggat
     4921 tacaagcccg agctgatcgc ccgccacgat cagccgctgc cgggtacggg cgagaaattc
     4981 attacgttcc agggcgagaa tggcaacgtc acgcgttcgc cgtgggaatt taagccccgc
     5041 ggcgaaagcg gcaagatgat ctcggacctc ttgccgaacc tggccgaact ggccgacgac
     5101 ctgtgcttcg tccattcgat gacggccaaa tcgaatacgc acggcccggc cgagaaccag
     5161 atgagcacgg gcttcacgct cgacggtttt ccgagcatgg ggtcgtgggt cagctacgct
     5221 ctgggctctg aaagccagga cctgccggca tttgtggcga ttcccgatcc ccgcggagtg
     5281 ccgcagatcg gccccaacca ctggaatagc gcgtttttgc ccgccgcgtt ccagggcacc
     5341 gccttcaacg ccgacaagcc gattcccaat ctcgccaggc cgcagcaatt cggtcccgcc
     5401 gccgaaaagg cgacgcgcga cctcttgaaa ctgctcaacg agcggcatct ggccgagcat
     5461 ccgggcgatt cgcagcttgc cgcccgcatc gccagctatg agctggccgc gcggatgcag
     5521 ctcggcgccg cggaggtcgg caatctggcc gaggagagcg aagcgacgca tcggctgtac
     5581 ggcacgggcg aagcgaacga cctgaaggcc cgcttcgccc gcaattgcct tttggcccgc
     5641 cggctgctgg agcggggcgt gcggtttgtg cagctcttca acggggccta cgccatgggc
     5701 gaaggcgtgg gcaactggga cggccaccgc aagctccacg agcagtacca ggtccacgcc
     5761 ccaatcctcg atcagccgtg tgccgccctg attcgcgacc tcaagagccg cggcctcttg
     5821 gggcacacgg ttgtcctgtt cgtcaccgaa ttcggccgca tgccgacgtt ccagaaaggg
     5881 gccagcggac gcgaccacaa ccctcacggc ttcacggcct ggctgacggg cgcgggggtc
     5941 aaacgcggct tcagttacgg cgccaccgac gaatttggcc ataaggcggt cgagaacgtc
     6001 gcgacgatct acgacctgca cgccacattg ctgcatttgc tcggcctcga acacgagcgg
     6061 ctcagtttct atcacaatgg aattgagcgg cggctgaccg acgtccatgg ccacgtgctg
     6121 acggacattc tcctttgagc gacggatgaa catgcgatcg acgtggattg ggacggtatt
     6181 gctgctggcg gcgtggctcc ctgcggccgt ttgcgcggca gacggcgatt cgtttgccga
     6241 aatcgagcgc gatttcgctg cccgggcaca gccgctggtc aagacgttct gcctcgattg
     6301 ccattcggct gccgcgaagg aaggcgaact ggacctggag cgattccgct cgctggccga
     6361 cgtgcgccgc ggcacaaccg tctggctcaa agtcgccgag atgctcgaca accgcgaaat
     6421 gccgccgaaa gatgccgagc ggcaactcac gggcgagcag cgccagcagc ttcgcggctg
     6481 gatcgagcga ttcctgcacg ccgaagcgct ggcgaccgcc ggcgatccgg gaccggtcgt
     6541 gctgcggcgg ctgaatagcg cggaatatac ctacacgctc cgcgatctga cgggcgtcga
     6601 ttcgctcgat ccggcccgcg agtttcccgc cgacggcgcg gcgggcgaag gcttcacgaa
     6661 cacgggcaac gccctggtga tgtcgccggc gctgatcacg aagtatctcg acgccggcaa
     6721 agaagtcgcc agccacgccg tgctcttgcc agacggattt cgcttttcgc cggccacgac
     6781 gcgccgcgac cggacgaacg aaacgctcgc gaggattcgc gagttttacg cgcagttcac
     6841 cgatgccggc ggcggcgaga aggtcaatct gcaaggcgtt gtcatcgaca cgaacgaagg
     6901 cggccggctg ccattggaag cgtaccttgc ggcgacgctc gccgagcgcg aggcgatcgc
     6961 gg
//
LOCUS       DASUGB010001160         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_685376, whole genome shotgun sequence.
ACCESSION   DASUGB010001160 DASUGB010000000
VERSION     DASUGB010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685376"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..644
                     /locus_tag="VFV87_22005"
     CDS             339..644
                     /locus_tag="VFV87_22005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5106516.1"
                     /translation="MVASLGWKRTKRITRLVTLPEFQGLGIAAQLAERVAAHEQAKGH
                     RVTITASHPAILAHCSRSPRWNYLGLKKTGSTRQRLAGRPIASSAGRAVASFEFVRP"
     gene            742..2241
                     /locus_tag="VFV87_22010"
     CDS             742..2241
                     /locus_tag="VFV87_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106517.1"
                     /translation="MLTAYPHPFCPHVPHPKQMEFLDLTCQEAFFGGAAGGGKSVALL
                     MAALQYVDQPGYSALILRKDLHRLALAGGLIPRSHEWLARSGAKWNGDRRQWTFPLAA
                     GPPATLTFGYLSGPFDKFRYASSEYQFIAFDELTEIREDDYLYLFSRLRRPTASSVPI
                     RVRAASNPGNIGHLWVKQRFVEGAEGVGWDKPAAGAGPPIVLGNADRIFIPSRIADNP
                     SLDEAEYRRTLMHLPPIDRERLMNGDWSVQEAGVFRAAWLRYYVETPSFNGEPQRSRR
                     VGCAHQGDDVLSREILVGGAHPTQLELFDSAGRLLAAVPERACRRFITIDPAGTSADK
                     QREARGRAASWSVVQVWDQPPRELSRFLLLRHQIRERVGFDGLVAMIRAAHARFGPQR
                     IWIEDEKLGQAAASTLSRAGLPIEVIRTQGRDKLTRAGELILKMERGEIFLPKLENGW
                     RTGFEAELLAWTGLESQPSDQIDAAAYAAILATQHNPRPIRIHPLVQRS"
     gene            complement(2333..2944)
                     /locus_tag="VFV87_22015"
     CDS             complement(2333..2944)
                     /locus_tag="VFV87_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106518.1"
                     /translation="MRFRLRTLLIVTAVLPPVLAAFWPLLPSSLGLPVVWLALAFMLA
                     VYLPLLLWIAASRPDAGASPTHDEPERAKLNESASIRVWGRPSLGGWLSLLNIMTWLA
                     LLTLLRCTADAGGMNREEWWTAPVMFTMVFMSLPLASCLLGPGGGGRGDPLSEAVTMA
                     IVLGLNAFAWGYGLALIVGGVRKWIAVLRPPVRARGESAKASG"
     gene            3206..3832
                     /locus_tag="VFV87_22020"
     CDS             3206..3832
                     /locus_tag="VFV87_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106519.1"
                     /translation="MLAADDAAAWSAFSPSAQAYKVLIPGTPQEETVFQDGQNVQVVT
                     ADGELPNGLFFMVITMEIPQKGSIQRREDALQFLAGAVESYHKRPGVQVLETSEIELG
                     KIPGRQVIADGFQGNKVRARHYAVGTQSYCLMAQSGDLELLNSDRAAKFFDSFELLNL
                     PAEPLSEHSRAYDLGRMAGAAICNAAIVVTVIVVLFGFLRRRKRGSTP"
BASE COUNT          703 a         1373 c         1211 g          632 t
ORIGIN      
        1 acgggcaggc gagacgccta ccccacgatg cggcgccgcc tcgtcggaac cgcgaccact
       61 ggtcgcggct ttgtggagct gtcttcagcg accgcccatc cgcctcgccg tcggccgcgt
      121 gccgcaaacc gcgtggcgaa cgtttgccag acatcactat ctagccggcg gactggcggc
      181 gtcggcgacc tgttatgccg ccttcctggc ggcgggggac gggggacggg gaacggggga
      241 caggggcaga gggactcaac gaacgggtca acgtcccttt gcccctgtcc cctgtcccct
      301 gttcccattc gcggagcgag ccgatcgcgt tctgtgcagt ggtcgcctcg ctcggctgga
      361 agcggacgaa gcggatcacg cggctggtca cgctgccgga gtttcaaggc ctgggcatcg
      421 cggcacagct cgcagaaagg gtcgcggctc acgaacaagc caagggccac cgcgtcacga
      481 tcaccgccag ccaccccgcg atcctcgccc actgctcgcg ctcgcccagg tggaactacc
      541 tcggcctcaa aaaaaccggc agcacgcgcc agcgcctcgc cggccgcccc attgccagca
      601 gcgccggccg cgccgtggcg agttttgagt ttgtccgccc ctagccggga ccaaacgtgg
      661 tcccggagaa ctgcgagcac gctccaccaa accaacccgc ccgctccggg agcaagtttg
      721 ctcccggcta aaagaatcgc catgctcacc gcctaccccc accccttctg cccgcacgtc
      781 ccgcatccca agcagatgga gttcctcgat ctcacctgcc aggaagcctt cttcggcgga
      841 gcggccggcg gcggcaagag cgtggccctg ctcatggcgg ccttgcaata cgtcgatcag
      901 ccgggctact cggccctgat tctccgcaaa gacctgcacc gcctggcgct ggcgggcggg
      961 ctcattcccc gctcgcacga gtggctcgcc aggtccggcg ccaaatggaa cggtgaccgc
     1021 cgccaatgga ccttcccgct cgccgccgga ccgcccgcca cgctcacgtt cgggtacctg
     1081 agcggaccct ttgacaagtt ccgctacgcc agcagcgagt atcagttcat cgcgtttgac
     1141 gagctgaccg aaattcgcga ggacgattac ctgtacctct tcagccgcct ccgccgcccg
     1201 acggccagca gcgtgccgat ccgcgtgcgc gccgcgagca acccgggcaa cattgggcac
     1261 ctgtgggtga agcagcggtt tgttgaaggg gcggaaggtg tagggtggga caagcccgcc
     1321 gcgggcgcag gcccaccaat cgtgctcggc aatgccgacc gcatcttcat cccctcccgc
     1381 atcgccgaca atccgtcgct ggacgaagcg gaataccgcc gcacgctgat gcacctgccg
     1441 cccatcgatc gcgagcggct gatgaacggc gactggagcg tgcaggaagc cggcgtcttc
     1501 cgcgccgcgt ggctgcgata ttacgtcgag acgccgtcgt ttaacggcga gccgcagcga
     1561 agtcgtaggg tgggctgtgc ccaccagggt gacgacgtcc tttcgcgcga aatcttggtg
     1621 ggcggagccc accctacaca gctcgagctg ttcgattccg ccggccgctt gctggccgcg
     1681 gtccccgagc gggcctgccg ccgcttcatc acgatcgacc ccgccggcac ctcggccgac
     1741 aaacagcgcg aagcccgcgg ccgcgcggcg agctggagcg tcgtccaagt ctgggaccag
     1801 ccgccgcgcg agctgtcgcg cttcctgctt ttgcggcatc aaatccggga gcgcgtcggc
     1861 ttcgacggcc tggtggcgat gatccgcgcg gcccacgcgc gctttggccc gcagcggatc
     1921 tggatcgagg acgaaaagct gggccaggcc gccgcgagca cgctttcgcg cgcagggctg
     1981 ccgatcgaag tcatccgcac gcagggccgc gacaagctga cccgcgcggg cgagctgatc
     2041 ctgaaaatgg agcgcggcga aatcttcctg ccgaagctgg agaacggttg gcggaccggc
     2101 ttcgaagccg agctgctcgc ctggaccggc ctggaatccc agccctccga ccagatcgac
     2161 gccgccgcct acgccgcgat cctcgccacc cagcacaacc cccgccccat ccgcatccac
     2221 ccgctcgtgc agcgctcctg acccaaagcc gcgaccagtg gtcgcggttc ccccgtaccc
     2281 tttctctgcc cttagccggg agcaaacctg ctcccggaga agtggtgcgg cgtcagccac
     2341 tcgctttcgc cgattccccg cgcgcacgga ccggcggtcg cagcaccgca atccacttgc
     2401 gaacgccgcc aacgatcaac gccaggccat agccccacgc aaatgcgttg agaccgagca
     2461 cgatcgccat ggttaccgct tcggacagcg gatcgcctcg accgcctccg cccggcccca
     2521 ggaggcaact cgccaacggc aacgacatga aaaccatggt gaacatgacg ggcgcagtcc
     2581 accactcctc ccgattcatt ccgcctgcat ctgctgtaca ccgcagcagc gtgagtagtg
     2641 ccaaccacgt catgatgttg aggagcgaca accacccgcc caaactcggc cggccccaca
     2701 cgcgaatgct ggcggactca ttcaatttcg cacgttccgg ttcatcgtgc gtcggcgatg
     2761 cgcccgcgtc aggccggctg gcggcaatcc acaagaggag tggaaggtaa acagcgagca
     2821 tgaaggccag ggcgagccaa acgacgggaa gccccaggct tgacgggagc agcggccaaa
     2881 aagccgccag cactggcggc aggaccgccg tcacgatcag cagcgtgcgg agccggaatc
     2941 gcatgcatgg atagtatgcc ggcgcccatc attccccaca gccgcgacca gtggtcgcgg
     3001 ttcccagtgg gggacgttgt aaggtggacg gtgcacacgg cttgatttcg cctcgcagcc
     3061 atggtgggta cggcctacca ggcaaagggt cgcgtcccca tctcgatatt ccgtggcccg
     3121 cgacttacca ttcgcagtgt cagccgaagg acgacttgcg atgcgattct gcctattgtt
     3181 tacgtggatt ggcattgtgc ccgccgtgct cgcagccgac gacgccgcgg cttggagcgc
     3241 gttctctccc tccgcgcagg cttacaaggt tctcataccc ggcactcctc aggaggaaac
     3301 cgttttccag gacggccaga acgtgcaggt ggtcactgcc gacggcgagc ttcccaatgg
     3361 gttgttcttc atggtcataa cgatggagat tccgcaaaag ggctcgatcc agcggagaga
     3421 ggacgcattg caattcctgg cgggagccgt cgagagctat cacaagcggc cgggagtgca
     3481 ggtgctggaa acgagcgaga tcgaactcgg aaagattccc ggccgtcagg ttatcgcgga
     3541 cggttttcag ggcaacaagg ttcgtgcgcg tcactacgca gttggcacgc agtcctactg
     3601 cctcatggcg cagagcggcg acctggaatt attgaactcc gacagggccg caaagttctt
     3661 cgattcattc gagctcttga atttgcccgc ggagcctctc agcgagcaca gccgcgccta
     3721 cgacctgggt cgcatggcgg gcgccgcgat ctgcaatgcg gcaattgtcg ttacggtgat
     3781 cgtggtgctg ttcggcttcc tgcgtcgccg gaagcgcggc tcaacgccat agcttaccgt
     3841 ccgccgctcc cgctcacacc ccgcccgctc ggcccttgcg gccgatcagc agatacagaa
     3901 tcgcgccgat ccagttcgt
//
LOCUS       DASUGB010001161         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_128477, whole genome shotgun sequence.
ACCESSION   DASUGB010001161 DASUGB010000000
VERSION     DASUGB010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128477"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1389)
                     /locus_tag="VFV87_22025"
     CDS             complement(160..1389)
                     /locus_tag="VFV87_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106520.1"
                     /translation="MTDVADGPGAAEVAEDVAADAKALEDAGSILTRDEAGHVTEVEL
                     NRDTGSDDDLAHLAGLPSVRVLTAESRGITDAGLAHLKDHPNLRAIKLGLTSVKDAGY
                     EHLQGLPRLEEIDLKRANISAEGYQHLAKIETLKRIRAPQTNTDDACLEAIKGMKQLE
                     LLDLQDVVLVTDKGLAVLEGFSKLKFLRLYGQTINDKVLSYMKNAKNLKILSLEQSAV
                     TADGLEHVRGLKNLEEVMLFGAKGVTDAALEKLSGLPKLKRLELRETPITSLGVSHLG
                     GLKALLVLDLAQTANVGNEGLEHIKGLENLQELNLWSTTIDDAGLASLSGLKNLKWLK
                     LDQCYITDEGLKHLAGLENLEYLHIGSTKVTDEGLEHLKGLKNLKTLVVTFVEGVSSD
                     GIDRLQESLPNLADVER"
     gene            complement(1641..2795)
                     /locus_tag="VFV87_22030"
     CDS             complement(1641..2795)
                     /locus_tag="VFV87_22030"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5106521.1"
                     /translation="MSQRIYLDNAATSWPKPEAVYRSVDVCQRRLGAPAGRGAYAEAS
                     EVERTVASCRKKIAQLIGAADPSRIILTQNGTDSLNLAIHGWLRPGDHVVTTVCEHNS
                     VLRPLRFLAEHREVTTTCVGCDSHGVVDPEGIRRAIQPNTRMIALIHASNVTGAIQPV
                     EEVGRIAAERGITYLVDAAQTLGHEPVDVRRIGCQLLAAPGHKGLLGPLGTGVLYIAP
                     GIDDRLLPLRQGGTGTRSDEDVQPSSLPDRYESGNLNVPGIVGLEAGVTEILTRGIEA
                     IRQHSQELVSRLIDGLREIPGITLYGSLTAHQRAGIVSLNVAGYDPQELASLLDSQWS
                     IQTRAGLHCAPRMHASLGTSPGGTLRLSVGPYNTPEQIEALAAALSEIAQ"
BASE COUNT          533 a          940 c          974 g          613 t
ORIGIN      
        1 ctggaccacc tcgaccgatg aaatgtctcg ccgtgcaatg cagcgagcaa tttcagccgc
       61 ggaccagtgg attgggctgt cggaaggatt catatgtggg acgggcttcc ggcctgccat
      121 gcaacccaat atcggcaggc aagatgcctg ccccgcggcc taccgctcca catcggctag
      181 attcggcagc gattcctgta accgatcgat gccgtcgctg gatacgccct ctacgaacgt
      241 cacgaccagc gttttgagat tcttcaaccc cttgaggtgc tccagcccct cgtcggtcac
      301 cttggtactg ccgatgtgca ggtattcgag attctccaat ccggccagat gcttcaagcc
      361 ttcgtcagtg atatagcact gatcaagttt cagccatttc aggttcttca ggccactcag
      421 gctggccagc ccggcatcgt cgatcgtcgt ggaccagaga ttcaattcct gcaagttctc
      481 gagccccttg atatgctcaa gcccttcatt gccgacgttt gccgtctgcg cgaggtcgag
      541 cacgagcagc gccttcaagc caccgaggtg agagacgccc aggctggtaa tcggcgtctc
      601 gcgcagctcc agccgtttga gctttggcag gccggatagc ttctccagtg ccgcatcggt
      661 gacgcccttg gccccgaaca acatcacttc ttccaggttt ttcagcccgc ggacgtgttc
      721 cagtccgtcg gcggtgactg cagactgctc cagcgacagg atcttcaggt tcttggcgtt
      781 cttcatgtac gacaacactt tgtcgttgat cgtctgcccg tacagtcgca ggaatttgag
      841 cttggaaaac ccctccagaa ctgcgaggcc tttgtcggtg actagcacca cgtcctgtag
      901 gtcgagcaac tcgagttgct tcataccttt gatcgcttcc aaacatgcgt cgtccgtatt
      961 ggtctgcggc gcccgtatgc gtttgagcgt ctcaatcttc gcgaggtgtt gatagccttc
     1021 cgcgctgatg ttcgctcgct tgagatcgat ttcttccagc cgcggcagcc cttgcagatg
     1081 ttcgtagcct gcgtctttga ccgaagtcag gcccagcttg atcgcccgca ggttcgggtg
     1141 gtctttcagg tgcgcgaggc ccgcatcggt gattccgcgg ctctcggcgg tgagcacgcg
     1201 gacgctcggc aggccggcca ggtgagccag gtcgtcgtcg ctaccagtgt cgcggttcaa
     1261 ctcgacctcg gtgacgtgcc ccgcctcgtc cctggtcaag atggagccgg cgtcttccag
     1321 cgccttggcg tcggcggcga cgtcctccgc gacctccgct gcaccgggtc catccgcaac
     1381 gtcggtcacc tcgggccgcg gcgaaaccgg cgccgacgac ggacaccccg ctgcggacac
     1441 gagaccggcg gagaccgcta gaaccataga aatccgcacg ccgattcgca ttactggcac
     1501 ccttgtgtgg ttggtttcca acggtttcag cctatctcat cgcttaggaa tcgcaaaccc
     1561 gggcaatcgg ccgaagcagt ggccgatggc tagttatctg gcggcgaaaa tgacggtcgg
     1621 caaaaatctt gcgagcgaga ctattgggcg atttcgctca gtgctgccgc taacgcttcg
     1681 atctgctcgg gcgtgttgta ggggccgacg ctgaggcgga gcgtgccgcc gggagacgtg
     1741 ccgagggagg cgtgcattct tggggcgcag tgcaggccgg cgcgggtttg gatcgaccac
     1801 tgcgaatcga ggagcgaggc caactcttgc gggtcatatc cggcgacgtt gaggctgacg
     1861 atgccggccc gctggtgggc ggtgagggaa ccgtagagcg taatgccggg gatctcgcgc
     1921 aagccgtcga tgaggcggga gactagctcc tgcgaatgct ggcgaatagc ttcgattccg
     1981 cgagtcagga tttccgtcac gccggcttcc aggccaacga tgccggggac gttgaggttg
     2041 cccgattcgt aacgatcggg gagcgacgac ggctgcacgt cttcgtcgct tcgggtgccg
     2101 gtgccgccct gacggagcgg gagcaggcga tcgtcgatgc cgggtgcaat gtaaagcact
     2161 ccagttccca gcgggccgag cagccccttg tgccccggcg cagcgagaag ctggcagccg
     2221 atgcggcgga catcgacggg ctcgtggccc aacgtttgcg ccgcatcgac gagatatgtg
     2281 atgcctcgtt cggctgcgat tctgccgacc tcctcgacgg gctggatcgc gccggtgacg
     2341 tttgacgcat ggatcagggc aatcatgcgc gtgttgggct gaatggcgcg ccgaatgccc
     2401 tcaggatcga ccacgccgtg cgagtcgcag ccgacgcagg tcgttgtaac ttcgcggtgc
     2461 tcggccaaaa accgcagcgg ccgcagaacg gaattgtgct cgcagacggt ggtgacgacg
     2521 tgatcgcccg gccgcagcca gccgtgaatc gccaggttga gggaatcggt gccgttctgg
     2581 gtgaggatga tccgactggg atcggccgcg ccgatgagtt gggcgatttt cttgcggcag
     2641 gaggcgacgg tccgctcgac ctcgctcgct tctgcataag cgccgcggcc ggcgggagcg
     2701 cccagcctgc gctgacaaac gtcgacggac ctgtacaccg cttccggctt gggccaggaa
     2761 gtggcggcgt tgtcgaggta aattcgctgg gacatacacg caggcgaggc aggagcctcg
     2821 cggacctgcg ttcccaggca gagcctggga acgagtaacg agatttacca ctcaccgctc
     2881 accgctcacc cgccgatctg atacatcgcc cgctcgggcc gaacgaagtc ggtgctgtcg
     2941 atgccgtgtc cgggcttttt ggcgcggacg cagtcgcgga taagctgggc gagctcttcg
     3001 ccgctgccgc caccgcggag gagcgcgcga gcgtcccatt caacggtcga aaacaggcaa
//
LOCUS       DASUGB010001162         5298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1935356, whole genome shotgun sequence.
ACCESSION   DASUGB010001162 DASUGB010000000
VERSION     DASUGB010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935356"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VFV87_22035"
     CDS             complement(<1..123)
                     /locus_tag="VFV87_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5106522.1"
                     /translation="MPKVTYKDAGVDLDVYDEAMTRLPRLMHRTFSPRVLKLDGG"
     gene            444..1766
                     /locus_tag="VFV87_22040"
     CDS             444..1766
                     /locus_tag="VFV87_22040"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106523.1"
                     /translation="MLELTSRRQSSNCEGASRRDFLKVGTLGLGTMGLGAMSLPALLQ
                     ARAAASASGKPVKETSVVWLWLSGGPTHVETFDPKLTAPSEYRSTTGEVATSIPGVTI
                     GGTLPQIASVADKMALVRSFAHTNSGHGGGTHYVMTGYDNRTVDNGGVPSRPGYGSIL
                     ARVRGPSNVATGIPTYVQFGGIRQGGGPAWLGAAYAPFDQDGQARKSMTLNMPEDHFN
                     DRRNLLTSLDGAKRSVEASDAAQGMKKFEQQAYNLVLGSATKAFDIQKEDAKTRERYG
                     KGLGEQMLAARRLCEAGCGFVTVQYGGWDMHGNIQRSMQQRSPQLDQAVAAFVNDVAE
                     RGLTDNILLVISGEFGRTPRVNRNAGRDHWAPLSTLAFSGGGLRMGQVVGESASKVDV
                     PGTKPIGPQDLMATIFHVLGIDPHLQFMSPSGRPVYMLEKGQPIAELV"
     gene            2024..3319
                     /locus_tag="VFV87_22045"
     CDS             2024..3319
                     /locus_tag="VFV87_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106524.1"
                     /translation="MPRFISTQLLASGLLLLLCGLAKVGGADESEANAAIAKLLQVGW
                     SGSFQSRAAADLQYQELQILAPGDPRALTASWLVLMQQRRYDEALKRIDECLAKQPGD
                     LEALRARAWTQAILKNYSATLLTAEKISAQMAEEPPISKADAGQHAALIEFLGRLCGY
                     LGGPVADQANQDQRKAAESQFLSRLSESHKPQFEKARDGVLAKFIELSDTKADERDKA
                     IETAQSDKEKTLQEVAAEREQIIARTEELDSRREKLQSELKDELSELGKQDAPLVQEL
                     ARLDARAAVIRRDLFAYQADIDRLERLAAGEKDPVRRQQLLFEADRLSVVAARIEADL
                     VGVNRLASGVQAQRAALAARAQQAQANTAGQVQRIDRELQDLAKRDKRADGIEKRAGR
                     PATGTTSKVRSLSAQATALTTYDQFPLEAAREKLLESLR"
     gene            3330..3698
                     /locus_tag="VFV87_22050"
     CDS             3330..3698
                     /locus_tag="VFV87_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106525.1"
                     /translation="MRILVCSNGCGEQLYGKKAAGWLPPHEAQAAINEGYHPYSYQAV
                     LWFTFFFVPIVPLGVYRVLEAHDTTFGGYRLTPVAWDWRQVMRHYLMALFGLACIPLA
                     LVAIVLLAWGLSSIAPPMTQ"
     gene            3714..3992
                     /locus_tag="VFV87_22055"
     CDS             3714..3992
                     /locus_tag="VFV87_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106526.1"
                     /translation="MSDDHRSSGSNAGVVIVIALLVVGLMCCGVGAFVGLSVFAYRSV
                     ERPPPPVLVQPMPGVEPQAVLDDGMEAAAPEDQPSAPAELPAQEPESK"
     gene            complement(4009..4434)
                     /locus_tag="VFV87_22060"
     CDS             complement(4009..4434)
                     /locus_tag="VFV87_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106527.1"
                     /translation="MIRSVCLSLAAVALLSIPAQADAYWGYYGWGGGPWSYGYNYNFG
                     TGYLPPPPYYSIFPPVYYSPHVTMRHYGASPYAWPAGFSPTAYAVRDYVARPAEPLMV
                     VNPYVRVENATAPAVAKSEEVEATPPLAIDNPHVASVAR"
     gene            4666..>5298
                     /gene="bioD"
                     /locus_tag="VFV87_22065"
     CDS             4666..>5298
                     /gene="bioD"
                     /locus_tag="VFV87_22065"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX5106528.1"
                     /translation="MKQNSNSALLERPRRRTSAAVRATVARRTARSGLFVVGTDTGVG
                     KTIVASRIVAALAQEGLRVGVYKPAASSCRRIGRALVSDDAVALWQAAGEPGSLKAVC
                     PQRFAAPLAPHLAARQERKEIDAKLLRQGLDYWRRRSDIVVVEGAGGLMSPIGENDYV
                     ADLAAEFGYPLVVVAPNQIGTINSTLLTLIAAAARPKPLPIAGIVLNDVLP"
BASE COUNT          978 a         1668 c         1735 g          917 t
ORIGIN      
        1 cccgccgtcc agcttcagca cccgcggcga aaacgtgcgg tgcatcagcc gcggcaggcg
       61 tgtcatcgcc tcgtcataga cgtccagatc gacgccggcg tctttgtaag tgactttggg
      121 cattgaggtt agggcgaaca acgacggctc ttgagccgag tgggctgtgg tgctccacac
      181 gcacgaaaga cggggaaatg cggcgacggg cgattgtcga atggactgca ccagttgtca
      241 acgacttcca tcggcgatgc ggcgatggca ttcatttcag ccctttccgc atcactggcg
      301 gtcgtcacgc agcgaacgca gaagtcgaga ttttccttga caaatcaacc gagattgatg
      361 taacaaagcg gttcggtcga tcattgtgat gcgtagggaa cctacccccg cccatttctt
      421 ctccagccga taggagagcc gccatgttgg agctgacttc ccgccgccaa tcgagcaact
      481 gcgaaggcgc tagccgccgc gactttctca aggtgggcac gctggggctg ggcacaatgg
      541 gactgggggc gatgtcgctg ccggccctct tgcaagcccg ggcggcggca tccgcgtcgg
      601 gcaagccggt gaaggagacg tcggtcgtct ggctgtggct ctcgggcggt ccgacgcatg
      661 ttgagacgtt cgacccgaag ctgacggccc cgtcggagta tcgcagcacg acgggtgaag
      721 tcgccacgag catccccggt gtgacgatcg gcggcacgct gccgcaaatt gcctcggtcg
      781 ccgacaagat ggcgctggtc cgctcgttcg ctcacaccaa tagcggacac ggcggcggca
      841 cgcactatgt gatgacaggc tacgacaacc gcaccgtcga taacggcggc gttcccagcc
      901 ggccgggcta tggctcgatc ctggctcgcg tccgcggtcc gagcaacgtg gcgacgggca
      961 ttcccacgta cgtgcagttt ggcggcatcc gccaaggcgg cgggccagcg tggctcggcg
     1021 ccgcgtatgc cccgttcgat caagatggcc aggcccgcaa gagcatgacg ctgaatatgc
     1081 cggaggatca tttcaacgac cgtcgcaacc tgctcacgtc gctggatgga gccaagcgga
     1141 gcgtcgaggc ctcggatgcc gcccagggga tgaagaagtt cgagcagcag gcctataacc
     1201 tggtgctcgg ctcggcgacc aaggcattcg acatccagaa ggaagacgcc aagacccgcg
     1261 agcggtatgg caaagggctc ggcgagcaga tgctcgccgc ccgccgcttg tgcgaagcgg
     1321 gctgcggctt cgtcaccgtg caatacggcg gctgggatat gcacggcaac atccagcgga
     1381 gcatgcagca gcgttcgccg caacttgacc aggcggtggc ggcattcgtc aatgacgtcg
     1441 ccgagcgcgg gctgaccgac aacattctgc tggtcatcag cggcgaattc ggccgcacgc
     1501 cacgcgtcaa ccgcaacgcc ggccgcgacc actgggcccc gctcagcacg ctcgcgttct
     1561 cgggcggcgg cctgcggatg ggccaggttg tcggcgagtc ggcgtccaag gtcgatgtcc
     1621 ccggcaccaa gcccattggc ccgcaggacc tgatggcgac gatcttccac gtcttgggca
     1681 tcgacccgca cctgcagttc atgtccccca gcggccgccc ggtctatatg ctggaaaaag
     1741 gccagccgat cgcggaactc gtgtagctct gcctttatag ctctgccttt agctgggacc
     1801 aaacctgcta ccaaacctgg tagcaaatct gggcccggag aattgccagt tgacgtcgat
     1861 tcgagacgca ccgggaccag gttttgtccc ggcgaagaac attcgccctc ctcgcggctt
     1921 tattccttgc cctcctgtcc agcgcccgat acgatgcgct gctgaggatc gtgcctcgcc
     1981 gaaacagggg tggtggggcc ggcgttcaag gctggaggta gtgatgcctc gtttcatttc
     2041 tacgcagctg ctggccagcg ggctgcttct cctactttgc gggcttgcaa aagtaggcgg
     2101 cgcggacgaa tccgaggcca acgcggccat cgccaagctg ctgcaggtcg gctggtcggg
     2161 aagctttcaa tcccgagcgg cagcggacct gcaataccag gagcttcaga tactcgcccc
     2221 gggggatccc cgggcgctga ccgcctcgtg gctggtgctg atgcaacagc ggcgatacga
     2281 cgaggccctg aagcgaatcg acgaatgcct cgccaaacag ccaggcgatc tggaagcgtt
     2341 gcgggcgcgg gcctggacgc aagcgattct gaaaaactac tccgcgactc tgctgacggc
     2401 ggagaagatc agcgcgcaaa tggccgagga gccgccgatc tccaaggccg atgcagggca
     2461 acatgcggcg ctgatcgagt ttctcggccg cctgtgcggc tacctgggtg gccccgtcgc
     2521 cgaccaggca aatcaggacc agcgcaaggc cgccgaatcg cagttcctct cgcggctcag
     2581 cgaatcgcac aagccgcagt tcgagaaagc tcgcgacggc gtgctcgcga agttcatcga
     2641 gctttcggat accaaggccg atgagcggga caaggcgatc gaaaccgccc agtccgataa
     2701 ggagaagacg ttgcaggaag tcgctgccga gcgcgagcag attatcgcgc ggaccgagga
     2761 gcttgattcc cgccgcgaga agctgcaatc ggagctgaag gatgagctgt cggagctcgg
     2821 caaacaggac gcgccgctcg tgcaggagtt ggcccgcctg gatgcgcgcg cggcagtgat
     2881 tcgccgtgat ctgttcgcct atcaggccga tatcgaccgc ctggagaggc tggccgcagg
     2941 cgagaaggac ccggtccgcc gccagcaatt gctctttgag gccgatcggc tgtcggtcgt
     3001 cgctgcgcga atcgaagcag atctggtcgg cgtcaatcga ctggccagcg gcgtgcaggc
     3061 ccagcgagcc gccctcgccg ctcgcgccca gcaagcccag gccaacacag cggggcaggt
     3121 ccagcggatc gaccgcgagc tgcaggacct cgccaagcgc gacaagcggg ccgacgggat
     3181 cgagaaacgc gccggccgtc ccgcgacggg aaccacgagc aaggtccggt cgctctcggc
     3241 tcaggccacc gcgctgacga cttacgacca gtttccgctt gaagcggccc gcgagaaact
     3301 tctggaatcg ctgcggtgag catcagtcta tgcgaatcct ggtttgctcg aacgggtgcg
     3361 gtgagcaact ctatggaaag aaagctgctg gttggcttcc tccgcacgaa gcccaagccg
     3421 caatcaacga aggctatcat ccttacagtt accaggccgt cctctggttt acgtttttct
     3481 tcgttccgat cgtgcccctg ggtgtatatc gtgtgctaga agctcacgac acgacttttg
     3541 gcggttatcg tttgacgccg gttgcctggg actggcggca agtcatgagg cactatttga
     3601 tggcgttatt cggcctcgcc tgcatcccat tggcgcttgt cgccatcgtg ctcttggctt
     3661 ggggattgag tagcatcgca cccccaatga ctcaataggc aaaggaccgg gctatgtccg
     3721 acgaccatcg atcttccggc tccaacgccg gcgtcgtgat cgtgatcgcc ttgctcgtcg
     3781 tcgggctgat gtgctgtggt gtcggagcgt ttgtaggcct gagcgtcttt gcctaccgct
     3841 cggtcgagcg tccgcctcct ccggtgctcg tccagccgat gccgggcgtc gaaccgcaag
     3901 cagtcctgga cgacggcatg gaagctgctg cgcccgaaga ccagcccagc gcgccggccg
     3961 aattgccggc gcaagaaccg gaatcgaaat agcagcacag attggccgct atcgcgccac
     4021 gctcgccacg tgcggattgt caatcgccag cggcggcgtc gcttcgactt cctccgattt
     4081 cgcgacggca ggcgcggtgg cgttttcgac tcgcacgtac ggattcacca ccatcagcgg
     4141 ctcggccgga cgagcaacgt agtcgcgaac ggcgtaggcc gtcgggctga agccggccgg
     4201 ccaggcatag gggctggccc cgtagtgccg catcgtgacg tgcgggctgt aataaaccgg
     4261 cgggaagatc gagtagtacg gcggcggcgg aagataaccc gtgccgaagt tgtagttgta
     4321 gccgtagctc cacgggcccc cgccccagcc gtaatagccc cagtaggcat cggcctgggc
     4381 gggaatcgaa agcagagcaa cagcagcgag cgacagacaa actgagcgga tcatggtgcg
     4441 aacctcaaac gtgggcggaa attccgacgc ggcgatgcgc ctctagctct cacggtaatt
     4501 gcccgctggg cgcgactcaa tggagtgccg gcgatttcgc tgtggggcag ggatcgggca
     4561 gggatcgctg tcaagaggcc agcgccatgt tcagtattct gaacgcccgc tcccgttctt
     4621 aaaccgctgt tgcgatcccc gagcggctgg tagcgtgaag gggtcatgaa acagaactcc
     4681 aacagcgctt tgctcgaacg tccacgccgc cgcactagcg ccgcggtgcg agcgaccgtc
     4741 gcgcgccgca cggctcgtag cgggctgttt gtcgtgggga ccgatacggg agtcggcaag
     4801 acgatcgtcg cttcgcggat tgtggccgca ctagcacagg aggggctgcg ggtcggcgtc
     4861 tataagccgg ccgccagcag ttgccggcgc atcgggcggg cgctcgtttc cgacgatgcg
     4921 gtcgctctgt ggcaagccgc gggcgagccc ggctcgctga aagccgtctg cccgcagcgg
     4981 ttcgccgccc ccttggcccc gcacctggcg gctcggcagg agcggaagga gattgacgcc
     5041 aagctgctcc gccaaggctt ggattactgg cgacgccgca gcgatatcgt cgtcgtggaa
     5101 ggggccggcg ggctgatgtc gcccatcggc gagaacgact atgtggcgga cctcgccgcg
     5161 gagttcggct atccgctggt ggtcgtcgcg cccaatcaga tcggcacgat caacagcacg
     5221 ctgctgacgc tgatcgccgc cgccgcccgg cccaagccgc tgccgatcgc cggcatcgtg
     5281 ctcaacgacg tgctcccg
//
LOCUS       DASUGB010001163         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1518289, whole genome shotgun sequence.
ACCESSION   DASUGB010001163 DASUGB010000000
VERSION     DASUGB010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518289"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFV87_22070"
     CDS             complement(<1..489)
                     /locus_tag="VFV87_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106529.1"
                     /translation="MPTISLTTFMKLCSKTPSSKATAYSKYLTPGGYDFYQRLKVEAN
                     AATVLGKSHSDCEEALASIANESERKHNLAGFKALVKWLITESGKFFAPPTALCESPK
                     GFIKVRLKPEFGIETAVGRRLVTLWNTKKPELTSSIAGVGIYLMNQHLLKGDFEDCKS
                     AVL"
     gene            complement(547..846)
                     /locus_tag="VFV87_22075"
     CDS             complement(547..846)
                     /locus_tag="VFV87_22075"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5106530.1"
                     /translation="MSNLIRHIIVRGRVQGVGYRAFVADEAMNRRLEGWVRNRRDGTV
                     EAVFSGPADAVQGMVRTCRKGPLGSRVESVHEWEGSAQELALASNKGQFSILLSD"
     gene            complement(843..1313)
                     /locus_tag="VFV87_22080"
     CDS             complement(843..1313)
                     /locus_tag="VFV87_22080"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5106531.1"
                     /translation="MTLHFAYGANMSRKVMRRHAPSAQPVGAGQLAGYRFVITPDGYA
                     SLAPCPAEVVHGVLWRLTARDRVTLDAWENVAAGLYRARTLPVRVQGRRAQALVYLAR
                     PGGEGRPKPGYMELVMAAGREWDLPDAYLQALERWLPAGPPKVKRPAAKIGEFG"
     gene            complement(1310..1834)
                     /locus_tag="VFV87_22085"
     CDS             complement(1310..1834)
                     /locus_tag="VFV87_22085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106532.1"
                     /translation="MNAVSLLFSRSGRLPPRPFAIAVIAVYAASFLSQALLGEPVLGR
                     AGLWPFALLQAVLVWIWLALHTKRLRDAGQPSGLAVGIAGLYVLSLVLLLLVLAVITT
                     TETGSSNFLLAGRGVIQIILVLYMFGVLAGSSDFGGLGVWLMGLVALLVLPVVLGIGY
                     SIWAGTRPSIPAEP"
     gene            complement(1857..>2311)
                     /locus_tag="VFV87_22090"
     CDS             complement(1857..>2311)
                     /locus_tag="VFV87_22090"
                     /inference="COORDINATES: protein motif:HMM:NF037204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2118 domain-containing protein"
                     /protein_id="HEX5106533.1"
                     /translation="IEGGIAVVMEGQAWPVVSSWVPGEPVWSGSVGGEWISVQVRPIP
                     NGFLLAHRGVETRAFVYTDTEAQAARLMPAKKASDTGKQVLCPMPGLVVSLAVTEGQE
                     VKAGETLAVVEAMKMENVLRAERDGVVKTIRVKPGDSLAVDAVIMEFA"
BASE COUNT          434 a          766 c          697 g          414 t
ORIGIN      
        1 gagaaccgcg cttttgcaat cttcaaaatc ccctttcagc aaatgttggt tcatgaggta
       61 gattccgaca cctgcgattg atgatgtcag ctctggcttt tttgtattcc acagagtgac
      121 gagtcgccga cccactgccg tttcaattcc aaattctggt ttgagtcgaa cttttatgaa
      181 gccttttgga ctttcacaaa gcgcggtcgg cggcgcgaag aattttccgc tttcggttat
      241 cagccacttc accagtgctt tgaaaccagc aaggttgtgc tttcgctcgc tttcattcgc
      301 gatcgaggcc aatgcttctt cgcagtctga atgcgacttg ccgaggacgg ttgcagcgtt
      361 ggcttcaact ttcagccgct ggtaaaaatc atatccgccg ggcgtgagat atttcgagta
      421 tgcggttgct ttgctcgaag gagtcttcga gcaaagcttc atgaaggtag tcaacgaaat
      481 ggtgggcata gacgcctcct agttcaccat tcgttctgat tcgctagcag tcgtcaagga
      541 cgagtgctaa tcgctcaaca ggattgaaaa ttggcctttg ttcgaggcga gcgccaattc
      601 ctgcgcgctg ccctcccact cgtggacgct ctcgacccgc gagcccagcg gtcctttccg
      661 acaagttcgg accatgcctt ggaccgcatc ggcggggcct gagaacacgg cctcgacagt
      721 gccgtcgcgg cggttgcgca cccagccttc gagccgtcgg ttcatcgcct cgtcggcgac
      781 gaaggcgcgg tagccgacgc cctggacacg gccgcggacg atgatgtgtc ggatgaggtt
      841 gctcatccaa attctccgat cttggccgca ggccttttga cttttggcgg tcccgccggc
      901 agccagcgct ccaacgcttg aagataggcg tccggcaggt cccattcccg cccggcggcc
      961 atcacgagtt ccatgtatcc aggcttgggc ctgccctcgc cgccgggccg cgccagatag
     1021 acgagcgcct gcgcgcgccg gccctggacc cgcaccggga gcgtgcgcgc gcggtaaagg
     1081 ccggcggcga cgttctccca tgcgtcgagc gtgacccgat cgcgggcggt gagccgccac
     1141 agcacgccgt gcaccacttc ggcaggacac ggagcgagcg aggcgtagcc gtcgggggtg
     1201 atgacgaagc gatagccggc aagctgcccc gcccccaccg gctgcgcgct gggcgcgtgc
     1261 cggcgcatta ctttgcggct catgttcgcg ccgtatgcga agtgcagcgt catggttcgg
     1321 cggggatgct tggccgcgta ccggcccaga tcgagtagcc gatccccagc acgaccggca
     1381 gcaccaagag cgccaccagc cccatcagcc agacgcccag gccgccaaaa tcggaactgc
     1441 ctgccagtac gccaaacatg tagagcacga gaatgatctg gatcacgccg cgcccggcta
     1501 aaagaaaatt gctgctgccg gtttcagtgg tggtgatcac agctagaacg agcaggagca
     1561 gcaccagcga cagcacgtag aggcccgcga tgccgaccgc gaggcccgac ggctggccgg
     1621 cgtcgcgcag ccgcttggtg tgcagagcca accatatcca gaccagcacg gcttggagca
     1681 gcgcaaacgg ccacagtccg gcacggccaa gcaccggctc accgagcagc gcctgcgaca
     1741 ggaagctcgc cgcataaacg gcgatgacgg cgatggcaaa cggccgcggc ggcaggcggc
     1801 ccgagcggga gaacaacagc gaaaccgcgt tcatgccgct tatggtcgca cggcgatcag
     1861 gcgaactcca tgatcaccgc atcgaccgcg aggctgtcgc ccggcttgac gcggatcgtc
     1921 ttcaccacgc cgtcgcgctc ggcgcgcagc acgttctcca tcttcatcgc ctcgaccacg
     1981 gccagcgtct cgccggcctt gacctcctgg ccctcggtca cggccaacga aaccaccagc
     2041 cccggcatcg gacagagcac ctgcttgccg gtgtcggacg ccttcttggc cggcatcagc
     2101 cgcgcggcct gcgcctcggt gtcggtgtaa acgaaggccc tggtctcgac gccgcgatgg
     2161 gccagcagga acccgttcgg gatcggccgc acctgcacgg aaatccattc gccgccgacg
     2221 ctcccggacc agaccggctc ccccggcacc caggatgaga cgaccggcca ggcttggccc
     2281 tccatgacga ccgcgatgcc gccctcgata t
//
LOCUS       DASUGB010001164         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_408302, whole genome shotgun sequence.
ACCESSION   DASUGB010001164 DASUGB010000000
VERSION     DASUGB010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408302"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFV87_22095"
     CDS             complement(<1..658)
                     /locus_tag="VFV87_22095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5106534.1"
                     /translation="MNALPAGSIDLVFADPPFNIGYDYDVYHDAKDYEEYLAWSRQWI
                     GAVHRVLKPDGTFWLAIGDEYAAELKLESQKLGFHIRSWVIWYYTFGVNCKTKFSRSH
                     AHLFHFVKDPKRFTFRAEELENRVPSARLLVYGDSRGNPDGRLPDDTWILRPQDLAGH
                     FSVEEDTWFFSRVAGTFKERRGFHGCQMPEQLLGRIIRICSHENEIVLDPFSGSATTL
                     A"
     gene            complement(886..1095)
                     /locus_tag="VFV87_22100"
     CDS             complement(886..1095)
                     /locus_tag="VFV87_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106535.1"
                     /translation="MASRPKLSPEPHRASLLRHRKRAYYARLPRPSLRLAAFALPGVN
                     RFGLHCRRADSWLAGADISLKRRAR"
     gene            complement(1107..1412)
                     /locus_tag="VFV87_22105"
     CDS             complement(1107..1412)
                     /locus_tag="VFV87_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106536.1"
                     /translation="MPAIRCQVLEVPAQPDWLPRRERPRDLLPWADPYIAVLMDRLED
                     RFDADRAFAADVDRDDPWSPPADYDWQDDAFRPDQPANAKLNWDPPVYGGWPLLDDQ"
     gene            1766..1957
                     /locus_tag="VFV87_22110"
     CDS             1766..1957
                     /locus_tag="VFV87_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106537.1"
                     /translation="MDVWDWVMLGLGAYVAVTSLVILMRRRRDEVLAGLAKDAEAARE
                     RKRQEELREAKKQKKQRAA"
     gene            2057..>3266
                     /gene="miaB"
                     /locus_tag="VFV87_22115"
     CDS             2057..>3266
                     /gene="miaB"
                     /locus_tag="VFV87_22115"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5106538.1"
                     /translation="MTRRLYIETVGCQMNVLDSEMVVADLRKRGYELARELAAADVIL
                     FNTCSVREQAENKTYSALGRLRTFKKEHPEKIIGVMGCMAQKDQQLVLKRAPYVDLVV
                     GPGQLARIPELIEEVAAGKGPQMAVSLGRKDGPIAEIKRSHETFDPLRDPTMRPTPFQ
                     AYLRIQIGCDKFCTYCIVPMTRGPEQGRPPEQILAEARILANQGCKEITLIGQTVNSY
                     RFTSGGRTTRLADLLQALSEIDGIQRLKFVTNYPKDMTDDLLQAVRDLPKCSPYLHVP
                     AQSGSNDVLARMKRGYTVEDYCEMMSRIRQTIPHAAVTSDFIVGFCGETEDDFQQTYD
                     LVAECRFKNSFIFKYSERPGTKGAELLKDDVPYEVKNRRNNDLLELQNRISEQDSQRF
                     LGERVEVLVEG"
BASE COUNT          665 a          970 c         1075 g          556 t
ORIGIN      
        1 ctgccagggt ggttgcgctg ccgctgaagg ggtcgaggac gatctcgttc tcgtgcgagc
       61 agatgcggat gatccggccc agaagctgct cgggcatctg gcagccgtgg aatccgcggc
      121 gctccttgaa cgtgccggcg acgcgggaaa agaaccaggt gtcctcctcg accgaaaagt
      181 gccctgccag gtcctgcggg cggagaatcc acgtgtcgtc cggcaggcgg ccatcgggat
      241 tgccgcggct gtcgccatag accagcaggc gggcggaagg aacgcggttt tccagctcct
      301 cggcccggaa cgtgaaccgc ttggggtctt tgacgaaatg gaacaggtgg gcgtgcgagc
      361 ggctgaactt ggttttgcag ttgacgccga atgtgtagta ccagatgacc caactccgga
      421 tgtggaagcc gagcttttgc gattcgagct tcagctccgc ggcgtactcg tcgccgatcg
      481 ccagccagaa cgtgccgtcg ggcttgagca cgcggtgcac cgccccaatc cactgccgcg
      541 accaggcgag gtattcctcg tagtccttag cgtcgtgata gacgtcgtag tcgtagccga
      601 tgttgaacgg cggatcggcg aagaccaggt cgatcgagcc ggccggtagt gcgttcatgc
      661 cggcgacgca gtcgccgtgg tggatctggc ccagaagttt ttcgtcaagg acttcgcggg
      721 gcaattcgct cgccgatcgc gagcggaggg aagggcgctt ggacatggcg tcgtgccgtg
      781 ctgggaacgg gtagaaatcg agcggctgcg tgcagattct agccgcagga tcattggagc
      841 gctagccgtg cgcgctgtcc agcactgaac atggccctaa ggccgctacc gagcccgccg
      901 cttcagagag atgtcggctc cggccagcca ggaatcggcc cggcggcagt ggagtccaaa
      961 gcggttaacg cccggcaagg cgaaggcagc cagccgcagg gatggccgcg gcagccgggc
     1021 atagtaagca cgcttgcggt gacggagcag actcgctcga tgcggctccg gcgaaagttt
     1081 cgggcgagag gccatcggac ggctccttac tgatcgtcca agagtggcca gccaccatag
     1141 accggcgggt cccagttcag cttggcgttg gcgggctgat cggggcggaa ggcatcgtcc
     1201 tgccagtcgt aatcagccgg cggcgaccag gggtcgtcgc gatctacgtc tgccgcgaac
     1261 gcccgatcgg cgtcaaatcg gtcttccagg cggtccatca gcacagcaat gtaaggatcg
     1321 gcccacggca gcaggtctcg aggacgctcg cgacggggga gccagtcggg ctgggcgggg
     1381 acttccaaaa cctggcaacg aatagcgggc atgggcaaac tccgttcgcg ggcgatgctg
     1441 ctcgagtggg cgaattcgcc ccgaggtgca tccgggctgc ccagactgga aagcaattcc
     1501 gatgccgtaa agttttcgac ctgccaagtc cgggaagaga atcgacataa cgtattgcta
     1561 cataatactt ttcgcgttct cggcagcgta cacccacttg aacaaggcct tgcgccattc
     1621 tgccgcggcc agtccgcatt ttttcaaaac cgtgccgctc tcgccttgga cggggaaatc
     1681 ccggtaagat ggcggcttgc agggacgaat ttgtagcaag tccgccccgt gcgacttgcc
     1741 caagtctgca gggagtgctc cgaacatgga cgtttgggat tgggtcatgc tggggctggg
     1801 ggcctatgtg gccgtcacgt cgctggtgat cctgatgcgc cgccggcggg acgaggtgtt
     1861 ggccggactg gccaaggacg ccgaggccgc ccgcgaacgc aagcgacagg aagagctgcg
     1921 tgaggcgaag aagcagaaga aacagcgagc ggcatagccg taggacgggt ttccaacccg
     1981 tccgccatag gatgggtctt gggcccgtcc cagaaatcaa gcaggtcggg ttgaaaaccc
     2041 aacctacgac ggtgccatga ccaggcgact ctacatcgaa accgtcggct gccagatgaa
     2101 tgtgctggac agcgagatgg tcgtcgccga cctgcgaaaa cggggctacg agctggcccg
     2161 cgagctggct gccgccgacg ttatcctctt caacacctgc tcggtccgcg aacaggccga
     2221 gaacaaaaca tactcggccc ttggccggct ccggactttc aaaaaggagc atcccgagaa
     2281 gattatcggc gtcatgggct gcatggctca gaaggaccag cagctcgtgc tgaagcgggc
     2341 gccgtatgtc gatctggtcg tcgggccggg gcagctcgcg cgaatcccgg aattgatcga
     2401 agaagtcgct gccggcaaag ggccacagat ggccgtcagc ctcggtcgca aggacggtcc
     2461 gattgccgag atcaagcgga gccacgaaac gttcgacccg ctccgcgatc cgaccatgcg
     2521 gccgacgcca tttcaggctt acctccgcat ccagatcggc tgcgacaagt tctgcactta
     2581 ctgcatcgtg ccgatgactc gcggcccgga gcaaggccgc ccgccggagc agattctggc
     2641 cgaagcgcgc atcctggcca atcagggctg taaagagatc acgctcatcg gccaaaccgt
     2701 caacagctac agattcacca gcggcggccg cacgacgcgg ctggccgatt tgcttcaggc
     2761 gctcagcgag atcgacggca tccagcggct gaagttcgtg acgaactatc cgaaggacat
     2821 gaccgacgac ctcttgcagg ccgtccgcga cctgccgaag tgctcgccct acctgcacgt
     2881 cccggcccag agcggcagca atgacgtgct ggcccggatg aaacgcggct atacggtcga
     2941 agactattgc gaaatgatga gccgcattcg gcagacgatt ccgcatgcag ccgtcaccag
     3001 cgatttcatt gtcggcttct gcggcgaaac agaggacgac tttcagcaga cgtacgacct
     3061 cgtcgccgag tgccggttca agaacagctt catcttcaaa tacagcgagc ggccgggcac
     3121 gaaaggggcc gagctgctta aggatgacgt gccctatgaa gtgaagaacc gccgcaacaa
     3181 cgacctgctg gagctgcaaa accgcatcag tgagcaggac agtcagaggt tcctgggcga
     3241 gcgcgtggaa gtgctcgtcg aaggtg
//
LOCUS       DASUGB010001165         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2076174, whole genome shotgun sequence.
ACCESSION   DASUGB010001165 DASUGB010000000
VERSION     DASUGB010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076174"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1599
                     /locus_tag="VFV87_22120"
     CDS             148..1599
                     /locus_tag="VFV87_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106539.1"
                     /translation="MSWIRAGARACPTMQELLMLRIHDARSRFGRRDFLQIGSLALGG
                     LALPQLLATQSLAAKANLRPVTGKSVIFLFLHGGPSQIETFDPKMSAPAENRSVTGEI
                     DTKLPGVTFGSTFERLAALNDKFTIVRSFRTGDANHDIKPVVSKATIGANMGSLFARI
                     AGTSDPVTGMPLNAALFPRAVDPERGPAVEQFGNFNATGGLGSAYAPFVPGGGGSLQQ
                     DMELKLDPTRIDDRRNLLGGLDRIRRELEARGEISGAGGFQQQAFQTILGGAAKAFDL
                     SQEDPKTIARYDTAPLISPEQISRKWNNYSRYVDNAKTLGKLLLLARRLCEAGCGFVT
                     VTTNFVWDMHADVNNATIEEGMRYMGHPLDHALSAFIEDCEVRGLSDKILLVATGEMG
                     RTPRLNARGGRDHWGGLSPLLLYGGGLNMGQVIGHSTPDAGEPLSQPYGNEDLLATVM
                     HTLLDVGQVRLMTGLPQDLLRAITTPEPIKGLI"
     gene            1813..>2121
                     /locus_tag="VFV87_22125"
     CDS             1813..>2121
                     /locus_tag="VFV87_22125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5106540.1"
                     /translation="MILETPRLLITDDDRDLRSTMAGLFQSRGFDTLEAGDGEEALEI
                     VGRQEIHLLLLDMQMPRMNGLETVRRLKELELALPMPWILLSGALDERIIEQAREAAA
                     F"
BASE COUNT          380 a          662 c          689 g          390 t
ORIGIN      
        1 ctcgaccgcg cctgggatga gtgctttgac tatcaccggc ccgctgagaa tcaaactgcc
       61 aatcaggaga tcttgagggc tgcggcgtaa gaacagtctc tgtttccatc aatcacgctt
      121 gattgatgtg ttcggctggg ccacaatgtg agctggatac gggccggcgc tcgcgcctgc
      181 cccacgatgc aggagctgct catgctgagg attcacgacg cccgcagccg ttttggtcgg
      241 cgcgattttt tgcagattgg cagtttggcc ctcggcggtt tggcgctgcc gcagcttttg
      301 gcgacgcaat cgctggcggc gaaggcgaac ttgcggccgg tgacgggcaa gagcgtgatc
      361 ttcctgttcc tgcacggcgg accgagccag atcgagacgt tcgatccgaa aatgtccgcc
      421 ccggctgaga accggagcgt cacgggcgag atcgatacga agcttcccgg cgtcaccttc
      481 ggtagcacgt tcgagcgatt ggcggcgctg aacgacaagt ttacgatcgt ccgcagcttc
      541 cgtacggggg atgccaatca cgacatcaag ccggtcgtca gcaaggcgac gatcggggcg
      601 aacatgggct cgctgtttgc ccggatcgcc ggcaccagcg accccgtgac gggcatgccg
      661 ctcaacgcgg cgctgtttcc gcgggccgtc gatccggagc gcggcccggc ggtcgagcag
      721 ttcggcaatt ttaacgcgac ggggggactg ggcagtgctt atgctccgtt cgtccccggc
      781 ggcggcggct cgctgcagca ggatatggag ctcaagctcg atccgacgcg aatcgacgac
      841 cgccgcaacc tgctcggcgg actcgaccga atccgccgcg agctcgaggc ccgcggcgag
      901 atcagcggcg ccggcggctt ccagcagcaa gcgtttcaga cgatcctggg cggcgcggcc
      961 aaggcttttg acctctcgca ggaagatcct aagacgatcg cccgctacga cacggccccg
     1021 ctcatctcgc ccgagcagat cagccgcaaa tggaacaact acagccgcta tgtcgataat
     1081 gcgaagacgc tgggcaagct gctgctcttg gcgcggcggc tgtgcgaggc gggctgcggc
     1141 tttgtaacgg tgacgacgaa tttcgtctgg gacatgcatg ccgacgtaaa caacgccacg
     1201 atcgaggaag ggatgcgcta catgggccat ccgctcgacc acgccttgtc tgcgttcatt
     1261 gaggactgcg aggtccgcgg cctctccgac aagatcctgc tggtcgccac cggcgaaatg
     1321 gggcgcacgc cgcgcctcaa cgcccgcggg ggccgcgacc actggggtgg cctttcgccg
     1381 ctcttgctct atggcggcgg cctgaacatg ggtcaggtga tcggccactc gacgcccgac
     1441 gccggcgagc cgctttcgca gccctacggc aacgaagatt tgctcgccac cgtgatgcac
     1501 acgctgctcg acgtcggcca ggtccgcctg atgaccggcc tcccgcagga cttgctccgc
     1561 gccatcacga ctccggagcc gatcaagggc ttgatttagg ggcacttgcc ccgccggtgc
     1621 cctgggatgc cgaattctac ggcagatttg actttgcggc cctggcagcg tttgatacaa
     1681 gtgtggcagc tatctgaagt tgcgctccgt cccgaagttt cacacgttta cggcactggg
     1741 cttgcaacgc aaggcggtag gctgcctagg gcctgccatg ttggccacga ctgaagtggc
     1801 aatgcgaccg atatgatcct ggaaactccc cgcctgctga ttaccgacga cgatcgcgac
     1861 ttgcgatcga cgatggcggg gctgtttcaa tcccgcgggt tcgacacgct ggaagcaggc
     1921 gacggggagg aggcgctgga aattgtcggg cggcaggaaa tacatctgct gctcctcgac
     1981 atgcagatgc cgcggatgaa cggcctggag acggtccggc ggctgaaaga gctggagctc
     2041 gccctgccaa tgccttggat tctcctttcc ggtgccctcg acgagcggat catcgaacaa
     2101 gcgcgcgagg ccgcggcttt c
//
LOCUS       DASUGB010001166         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1381846, whole genome shotgun sequence.
ACCESSION   DASUGB010001166 DASUGB010000000
VERSION     DASUGB010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381846"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="VFV87_22130"
     CDS             <1..1803
                     /locus_tag="VFV87_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX5106541.1"
                     /translation="RAENEPKLISVTVSPTENFLKAGEESPLAVTATYSDGSTRDVTS
                     RTQYQSSEMGLVSVSPAGVVSAGPIPGEATIMARYMNLIATCHVAIPLPGEVTAELYA
                     GLPRQSFIDEHVWTKLQSLGITPSAPCDDARFLRRVHLDLIGRLPTPDEVRAFLADGD
                     SQKRARIVDELLQRPEYADHWAAKWADLLRPNPYRVGIKAVLNYDAWIRESFRQNKPY
                     NQFVRELLTAQGSSWENGAAVMFRDRREPPEITTIVSQLFLGIRLECARCHHHPFEKW
                     GQDDFYSFAAYFARLGHKGQGISPPISGGEETVLLRKTGSVEHPLTGQMLQPRPLFGE
                     APKIEGDADPRVALAAWITSDENPFFAQVIVNRIWADLMGRGLVEPVDDLRATNPPTN
                     GPLLEALAADFRAAKYDLKHLLKSICTSHVYSLSSLPGERNVADRQNYSRHYRTRLRA
                     EVLLDAVTDISGVPDSFQAMPGGSRANQLWTTRVDSLFLDTFGRPNPNQDPPCERTPD
                     TTVTQTLHLMNAPQLHKRVTSDDSRAAQLAASQLSPDQIVEEIYLLVYSRLPDEAERE
                     IGRGLFAESGTSRRQAAEDLMWALLNTPEFMFKD"
BASE COUNT          373 a          647 c          624 g          360 t
ORIGIN      
        1 cgcgccgaga atgagccgaa gttgatctcc gtcacggtga gcccgactga aaacttcctg
       61 aaggccggcg aggaatctcc actcgccgtc accgcgacgt actctgacgg ctctacgcgc
      121 gacgtgacca gccgcacgca atatcagtca agcgaaatgg gtctagtgag cgtgtcgccc
      181 gcgggcgtgg tttccgctgg gccgattccg ggcgaggcaa cgatcatggc ccggtatatg
      241 aacctgatcg caacctgcca cgtcgcgatt ccgctgccgg gcgaagtgac ggcggagctg
      301 tacgccggcc tgccgcggca gagcttcatc gacgagcacg tctggaccaa gctgcagtcg
      361 ctgggcatca ctccttccgc gccgtgcgac gacgccaggt tcctccgccg cgtgcatctc
      421 gatctgatcg gccggctgcc gacgccggac gaagtgcggg cgtttctggc cgacggcgat
      481 tcgcagaagc gggctcgcat tgtcgatgaa ttgctccagc ggcccgaata tgccgatcac
      541 tgggcggcca agtgggcaga cctcttgcgg cccaatccgt accgcgtcgg catcaaggcc
      601 gtgctgaact acgacgcctg gattcgcgag agcttccggc agaacaagcc gtacaaccag
      661 ttcgtccggg aactgctcac cgcccagggc tctagctggg aaaacggggc cgcggtcatg
      721 ttccgcgacc gacgcgagcc gccggagatc accacgatcg tcagccaact cttcctgggc
      781 attcggttgg aatgtgccag gtgccaccac catccattcg agaaatgggg gcaggacgat
      841 ttctatagct tcgccgccta ctttgcccgc ctcggtcaca agggccaggg catttcgcct
      901 cccataagcg gcggcgaaga gacggtcctc cttcgcaaga cgggctcggt cgagcatccg
      961 ctcaccggcc agatgctcca gccgcggccg ctgtttggcg aagcaccaaa gatcgaaggc
     1021 gatgccgatc cgcgtgtcgc gctggcggcg tggatcacgt ccgatgagaa tccgttcttc
     1081 gcgcaagtga tcgtcaaccg catttgggcc gatttgatgg gccgcgggct cgtcgagccg
     1141 gtcgatgacc tgcgggcgac gaacccacca acgaatggtc cgctgctgga agcgttggcc
     1201 gccgatttcc gggcggcgaa atacgacctg aaacatctac tgaagtcgat ctgcacgtcg
     1261 cacgtctatt cgctcagctc gctgccgggc gaacggaacg tggccgaccg gcagaattac
     1321 tcgcggcatt accgtacacg tttgcgggcc gaagtgctgc tcgatgcggt gaccgacatc
     1381 agcggcgttc ccgactcgtt ccaggcgatg ccgggcggct cgcgggccaa tcagctttgg
     1441 acgacgcggg tcgattcgct gttcctcgac accttcggcc ggccgaatcc gaatcaggat
     1501 ccgccctgcg agcggacgcc cgatacgacc gtcacccaga cgctccacct gatgaacgcc
     1561 ccgcagcttc ataagcgcgt cacttccgac gacagccggg ccgcgcagct tgccgccagc
     1621 cagctttcgc cggaccagat cgtcgaggag atttacctgc tggtctattc ccgcctgccg
     1681 gacgaggcgg aacgtgaaat cggccgcggc ttgttcgcgg aaagcggaac ttcccgccgc
     1741 caggcagccg aagatttgat gtgggcgcta ctgaatacac ccgagttcat gttcaaggac
     1801 tagcatcgtt taacccgcag ccgtaggcgc aagcgaatgg atgttccatc cggaagacgc
     1861 ctgcgcctcc gcttcgctgc ggctacgggt taaacgaagg aggactgcga tgacgacgca
     1921 ccgcaactgc gaaggttttt cacgccgtga ctgcatcaaa ctcggcttgg gtggactctt
     1981 gtcgggcggc ctggttgggg cgct
//
LOCUS       DASUGB010001167         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1660955, whole genome shotgun sequence.
ACCESSION   DASUGB010001167 DASUGB010000000
VERSION     DASUGB010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660955"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFV87_22135"
     CDS             complement(<1..203)
                     /locus_tag="VFV87_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5106542.1"
                     /translation="MNNVTQILARIEQGDQAAAEQLLPLVYDELRKLAAAKMTQENPG
                     QTLQATALVHEAYLRLVAGDESKP"
     gene            complement(219..962)
                     /locus_tag="VFV87_22140"
     CDS             complement(219..962)
                     /locus_tag="VFV87_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX5106543.1"
                     /translation="MAKLHSVPVSCSICKQSKPRGEFAAGETLRDAVVAELRRIHADW
                     APDQPICYACVNRFRAEHFRRLLEEERGELSSLEEQVINSLREQEALSENVNLQFERQ
                     LSFGERLSDRLAAFGGSWSFLILFAVVVIAWMIGNGIFLAQRPFDPYPFILLNLVLSC
                     LAAIQAPVILMSQNRQEAKDRLRSEHDYRVDLKAELEVRLLHTKMDQLLTHQWQRLLE
                     IQELQMEILEDVANRKTGRTPSPKPRDAE"
     gene            complement(1001..2068)
                     /gene="epmB"
                     /locus_tag="VFV87_22145"
     CDS             complement(1001..2068)
                     /gene="epmB"
                     /locus_tag="VFV87_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEX5106544.1"
                     /translation="MEIVAGRGKAVGGTCDSPSHPWPLWRQGRGEQRTWKQELRLAIR
                     DAAQLLAALELPADAAPLAQNAADFPVFAPWPYVARMRKGDRADPLLRQVLPLVDELA
                     EQPGFAADAVGDEAALAAPGLLQKYKGRALLVTTGACAVHCRYCFRRHFPYSESPRSI
                     DDWQPAIDHIAADPTIDEVLLSGGDPLTLVDEQLAELARRLAAIPHLRRLRIHTRLPI
                     VIPQRVTGALLGWLQTTRLTPILVVHANHPQEIDEAVAAALTALVDAGIPVLNQSVLL
                     RGVNDNADALIGLSRRLIDLRVMPYYLHQLDRVQGAAHFEVPIRRGLELIAEMRRALP
                     GYAVPRYVQEIAGEEHKRVLA"
     gene            2117..2686
                     /gene="efp"
                     /locus_tag="VFV87_22150"
     CDS             2117..2686
                     /gene="efp"
                     /locus_tag="VFV87_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5106545.1"
                     /translation="MATYKTGDFRKGIKVMIDNEPWLMVEMNFRKPGKGNALYECTLK
                     NLIRGTSLKRVYRGGDELEAADVEEVACTYLYRQGDTFVFMNSKTYDQYELTAEQVDE
                     YWKYLKEGMAVQTMLFNGLPISITPPPHVELKVEYCEPGARGDTATNVTKPCKMETGA
                     EILAPLFIQIGNVLKIDTRTGEYIERVST"
BASE COUNT          577 a          925 c          954 g          521 t
ORIGIN      
        1 ggcttggatt cgtcacccgc aaccagccgc aggtacgctt catgcaccag cgccgtcgcc
       61 tgcagcgtct gacccgggtt ctcctgggtc atcttggccg cggccagctt gcgcagctcg
      121 tcgtacacca gcggcaagag ctgctccgcc gcggcctggt cgccttgctc gatgcgggcg
      181 agaatttgtg tgacgttgtt catggcgaat tcgctccgtc actccgcatc tcgcggcttg
      241 ggcgatggcg tgcggccggt cttccgattg gcgacatcct ccaggatttc catctgcagc
      301 tcttgaatct ccaggagccg ctgccactgg tgggtgagca gttgatccat tttcgtgtgc
      361 agcaggcgca cctccagctc cgctttcagg tccacgcggt agtcgtgttc cgatcggagc
      421 cgatccttgg cctcctggcg attttggctc atgaggatca ccggagcttg aatggctgcc
      481 aggcaggaga gcacgaggtt gagcaggatg aacgggtagg gatcgaacgg gcgctgggcc
      541 agaaagatgc cgttcccaat catccacgcg atcacgacga cggcaaagag gatcaagaag
      601 ctccagcttc caccaaacgc tgcgagccgg tccgacagcc gttcaccgaa cgacaactgt
      661 cgctcaaatt gcaggttgac gttctccgac agcgcttcct gctcgcgcag gctgttaatg
      721 acttgctctt ccagcgatga caattcaccc cgttcctcct ccagcagccg gcggaaatgc
      781 tcggcccgaa agcggttcac gcaggcatag cagatgggct gatcgggggc ccaatcggcg
      841 tggatgcggc gcagctccgc gaccacggca tcgcgcaggg tttccccagc cgcgaattcg
      901 ccgcgcggct tgctctgttt gcagatcgaa caagaaaccg ggacggaatg cagcttcgcc
      961 ataagaatgc tcggctggaa aggctggaag acggacggac ctacgccaaa acccgtttat
     1021 gttcttcgcc ggcgatttcc tggacgtagc gcgggacggc gtagccggga agggcgcggc
     1081 gcatctcggc gatcagctcc aggccgcggc ggatggggac ttcgaagtgg gcggcgcctt
     1141 ggacgcggtc gagctggtgc aggtaatagg gcatgacccg caggtcgatc agccggcgcg
     1201 agaggccgat gagcgcgtcg gcgttgtcat tgactccacg gagtaagacc gattgattca
     1261 ggacggggat gccggcatcg acgagcgcgg tcagcgcggc ggcgaccgct tcgtcgatct
     1321 cttgcggatg attggcatgc acgaccagga tcggcgtcag ccgcgtggtc tgcagccagc
     1381 ccagcagcgc gcctgtgact cgctgcggaa tgacgatcgg caggcgggtg tggatccgca
     1441 accgccggag gtgtgggatt gccgccagcc ggcgggccag ctcggcaagc tgctcatcga
     1501 caagggtgag cgggtcgccc cccgagagca gcacttcgtc aatggttgga tcggcggcga
     1561 tgtggtcgat tgccggctgc cagtcgtcga tcgaccgggg gctttcggaa tacggaaaat
     1621 gccggcgaaa gcagtagcgg caatggaccg cgcaggcccc ggttgtgacg agcaaggccc
     1681 ggcctttata cttctgtaac agcccgggag cggcgagcgc tgcctcgtct cccacggcat
     1741 cggccgcgaa gccaggctgc tcggccagtt cgtccacgag cggaagcact tgcctcagga
     1801 gggggtcggc cctgtcgccc ttccgcatgc gggcgacata gggccaggga gcgaaaaccg
     1861 gaaaatctgc ggcattctga gccagcgggg cagcatccgc aggaagctcc agtgccgcca
     1921 gcagttgcgc cgcatcgcgg atggccaggc gcagctcctg cttccaggtc cgctgctccc
     1981 ctcgcccctg gcgccagagg ggccaggggt gagagggcga atcgcacgtc ccgccgacag
     2041 ctttgccccg tcccgctaca atttccatct aatcacaata accgtcgcac tttacaaatc
     2101 gaagtaggga acagtcgtgg ccacgtacaa gacaggcgat tttcgcaagg ggatcaaggt
     2161 gatgatcgac aatgagccct ggctcatggt cgaaatgaac ttccgcaagc cgggcaaggg
     2221 aaacgccctg tatgaatgca cgctgaagaa cctgatccgc ggcacctcgc tcaagcgggt
     2281 ctatcgcggc ggcgacgagc tggaagcggc cgacgtcgag gaagtcgcct gcacttatct
     2341 ttaccgccaa ggcgacacgt tcgtcttcat gaacagcaag acctacgacc agtacgagct
     2401 gaccgccgag caggtcgatg agtactggaa gtacctcaag gaaggcatgg ccgtgcagac
     2461 gatgctcttt aacggcttgc cgatcagcat cacgccgccg ccccacgtgg agctgaaggt
     2521 cgaatactgc gagccgggcg cccgcggcga tacggcgacg aacgtcacca agccgtgcaa
     2581 aatggaaacg ggggccgaaa tccttgcgcc gctgttcatt cagatcggca acgtgctgaa
     2641 gatcgacacg cggaccggcg agtacatcga gcgcgtttcg acgtaggttc cgacggccgc
     2701 ggcggaacca gcgccgcgag cacatgccgc gtgctgagag agtttgctcg ccaaacatgg
     2761 ccggtactcg catcaggtcc cgcccggcgg tcgggaccta cagcgcaagc gcaacgagca
     2821 tagtgcattt cggccgcagc ggcatcaggc gccctacgcc cgtagcgtca aaacttgccc
     2881 tgctctccct tccgccactc ctcgtcaaga attcgcgaaa atccgtcgcc ggagccgcac
     2941 ccccgatttg gcgtttgccc gctggcaatt agaattg
//
LOCUS       DASUGB010001168         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1382013, whole genome shotgun sequence.
ACCESSION   DASUGB010001168 DASUGB010000000
VERSION     DASUGB010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382013"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFV87_22155"
     CDS             <1..390
                     /locus_tag="VFV87_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106546.1"
                     /translation="INRQPFMLAQALVMVARSGDAKHLPEVEKLLEDASVITRMQENN
                     GNQGKPVMYEIQVLDVALATAALLSKQELKAYFENAPQPDQTTDWQQVFFNARVIGSS
                     SDEQRTKTFAKWAAFKAKQGTPEAASP"
     gene            642..1253
                     /locus_tag="VFV87_22160"
     CDS             642..1253
                     /locus_tag="VFV87_22160"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX5106547.1"
                     /translation="MPRLLVTSLVIFASCSATGCRTYGERGALLGGLTGAAIGGAIGE
                     DSGNAVPAALIGSAVGAVTGGAIGDNVDADLARSRAEIEARMGRQMRGAVTVADVVAM
                     TQAGLSQDVIATHIRANGVAQPPQVNDLIYLRSQGVGDFVIQTMQQAPPPAVAPVVYG
                     PPPGAVVVEHHYGPWYPPPPAPFFHYHNHGYRGRAHWGFSFCR"
     gene            complement(1292..>2139)
                     /locus_tag="VFV87_22165"
     CDS             complement(1292..>2139)
                     /locus_tag="VFV87_22165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5106548.1"
                     /translation="VVDNNCTDDTREVVQSFKALEAFPCLRLLHEGRQGVGHARRTGL
                     RAARGRLIGFVDDDCLVDSHWIAAALEFADSHCQAGAFGGRNELIWQAPPPDFALAYG
                     ESLARQDWGAIEFQLSSAGRRVPCGAGLVLRRTALDAGGWLDVGCLRGRDPDFLGAGE
                     DAEIALRIRNAGWQIWYAPQLRLTHLIPPQRMTLSYVRRLHGGFGRAEVFLRHLAQRR
                     NDSWAARLRGLVWSLTELLAVVRRFWLGYIWYSAERPSWLIRLSYATGCLEGGVRFLL
                     TGKAQ"
BASE COUNT          399 a          694 c          696 g          350 t
ORIGIN      
        1 atcaaccggc agccgttcat gctggcgcaa gccctggtga tggtggcccg ctcgggcgac
       61 gccaagcacc tgccggaggt cgagaagttg ctggaagatg ccagcgtcat tacccgcatg
      121 caggaaaaca acggcaacca gggcaagccc gtaatgtatg aaatacaagt gctggatgtg
      181 gctctcgcca cggcggcgct gctgagcaag caggaattga aggcgtactt cgaaaacgca
      241 cctcagcccg atcagacaac ggattggcag caagtcttct tcaacgcccg agtgatcggt
      301 tcttccagcg atgagcagcg gaccaaaacc ttcgcgaagt gggctgcatt caaggccaag
      361 caaggcacac cggaagctgc ctcaccctag gcttctcgct gcgcgagaag caatggactt
      421 gccactggaa ggaacgtcgc gctcgcttga gcgggccatc gcgaggagtg cttcgccggc
      481 gcgacctccg cttgccaaat acatcccttg cgactcgcgg agcgagtcgc ctacggtcgc
      541 tgctagcagg tggaattcgc aggccttgac gcggaacccg cccgcaagga atatcccggc
      601 ccttccgaag tcttcgtacc cggttgcagg gacgcttccg catgcctcgc ttgctggtca
      661 cttcgttggt gattttcgcc tcgtgttccg ccaccggctg ccggacgtat ggcgagcgcg
      721 gcgcgctctt gggcggtttg acgggcgcgg cgatcggcgg agcgatcggc gaagatagcg
      781 gcaacgcggt tcccgcggcc ttgatcggct cggcggtcgg cgccgtgaca ggcggggcga
      841 ttggcgacaa cgtcgatgcc gatctcgccc gctcgcgggc cgagatcgag gcgcggatgg
      901 ggcgccagat gcgcggcgcg gtgaccgtcg ccgacgtcgt cgccatgacg caggccggcc
      961 tcagccagga cgtgatcgcc acgcacattc gcgccaacgg cgtcgcccag ccgccgcaag
     1021 tgaacgacct gatctacctc cgcagccagg gcgtgggtga cttcgtgatc cagacgatgc
     1081 agcaggcccc gccgccggcc gtcgcgcccg tcgtttatgg tccgccgccg ggagcggtcg
     1141 tcgtcgaaca tcactacggg ccctggtatc cgccgccgcc agcccctttc ttccactacc
     1201 acaaccatgg ctaccgtggc cgggctcact ggggattctc gttctgtcgc tgagttgata
     1261 tcggccattt gctcaccgaa aatggcccgg cctactgtgc cttcccggta agcaggaatc
     1321 gcacgccgcc ttccagacag cccgtggcgt agctgaggcg aatcagccaa ctggggcgct
     1381 cggcagaata ccagatatag ccgagccaga agcggcggac gacggcgagc agttcagtga
     1441 gcgaccagac cagaccgcgg aggcgagccg cccacgaatc gttgcgtcgc tgggccaagt
     1501 gccgcaggaa tacctcggca cggccaaagc cgccgtgcaa ccgccgcaca taggaaagcg
     1561 tcattcgctg tggcgggatg agatgtgtca accgcagttg cggggcgtac cagatttgcc
     1621 agccggcgtt gcggatgcgg agggcgattt cggcgtcttc gccggcgcca aggaaatcgg
     1681 gatctcggcc gcggaggcaa cctacgtcca gccagccgcc cgcatccagc gctgtgcgcc
     1741 gcagcaccag tcccgcgccg caggggacgc gccggcccgc ggaagagagt tggaactcga
     1801 tggcgcccca atcttgacgg gccaggcttt ctccgtaggc gagggcaaaa tccggaggcg
     1861 gcgcttgcca tatcaattcg ttgcggccac caaaggcgcc ggcctggcag tgtgagtcgg
     1921 cgaattccag tgcggcggcg atccagtgtg aatcgaccag gcaatcgtca tcgacaaagc
     1981 caatcagccg cccgcgggcc gcccgcagcc cggtacggcg ggcgtggcct acgccttgtc
     2041 gcccttcgtg cagcagacgc aggcagggga aagcttccag agctttaaat gactgcacga
     2101 cctcgcgcgt gtcatcggtg cagttgttat cgacgacca
//
LOCUS       DASUGB010001169         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1241971, whole genome shotgun sequence.
ACCESSION   DASUGB010001169 DASUGB010000000
VERSION     DASUGB010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241971"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1262
                     /gene="lhgO"
                     /locus_tag="VFV87_22170"
     CDS             57..1262
                     /gene="lhgO"
                     /locus_tag="VFV87_22170"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX5106549.1"
                     /translation="MAEPHVAIVGGGIVGLATAWQLQNRYPRIRVTVIEKEAEVGEHQ
                     TGHNSGVLHSGIYYKPGTLRAANCRTGKRAMEEFCAEHGVPYDICGKVIVAISEPELP
                     LLERIFERGQQNCVRCEMIGPQRLKELEPHTPGIRAIHVPEAGIVNFREVARKLAELV
                     KMRGGEVRCNARVIAVHHDEGAITVESTAGEVQADYLVTCAGLQSDRVAKLTGAQPEA
                     KVLPFRGEYYDLKPEAQHLCRNLIYPVPDPNFPFLGVHFTRMIEGGVECGPNAVLAFA
                     REGYRKTDFNCRDFSEALAYRGFRKLAWKYWRTGAGEMWRSWSKAAFVKALQRLIPEI
                     RSEHLVAGRSGVRAQAISPAGSIVDDFVIEARGQIVNVLNAPSPAATSSLNIGKLIVE
                     KLAEQLTVR"
     gene            1299..1961
                     /locus_tag="VFV87_22175"
     CDS             1299..1961
                     /locus_tag="VFV87_22175"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5106550.1"
                     /translation="MSIASEARVIWDAEMLAQRFGPIPLDRICGEPAPGLGTEDDVIR
                     FNERENRLFELVDGVLVEKTMGAYESMLAVEIASLLRNWVKPRKLGTVLGSDGILRLF
                     AGLIRIPDVAFLSMEKFPRGRFPRDKAPPLAPDLAVEVLSESNTKKEMAEKLRDYFAA
                     GSKLVWYVDPERREFTVFTSPTDSRVVTEQGTVDGGQVLPGFTMSVAELFAELPLEDE
                     ES"
     gene            complement(1984..3396)
                     /locus_tag="VFV87_22180"
     CDS             complement(1984..3396)
                     /locus_tag="VFV87_22180"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5106551.1"
                     /translation="MFTTRAVPGLALFPLILFTLQTVKAQVPDLDKELRDRQIQRLFT
                     AAQLGDCRELDRLLRLHPELVNASYPYHFSGIHGWTVLHCAVVADDCQLARLLLSRGA
                     DPNSECATRDRPLHMLQSAKMGRLLLAAGADANGRGAWEERPIHHARTLEIARLLVAH
                     GARVNDQDNDWRGSPLHHACRWKRLRVAQYLLSHGADIAAKNDWQRTPLHEAAASGDS
                     RIVKLLLHEGAEVNARDEEEYTALHSACAALSPLAVDLLLAAGADARAASKSGITPLL
                     WAAAAGEGEADAAAQLAIIEQLVKHGANLHATNADGQSALHFAASEGRLEVVEYLLKQ
                     RLSANAKDRLDRTPLHDAMELHRTITFGLRGDGSREVAITRTLIEHGADIKAVAYLED
                     TEHVGEGDEVRTIVIRRPVTLRQLAQHKGLDWSPDDEGFNQFPLQGGDVNRMVTQWRT
                     RNAVKQEFLEMLDQANPGEE"
BASE COUNT          636 a         1058 c         1074 g          673 t
ORIGIN      
        1 gccagcgtgg aagaaaagct cggccgcagc ctgaagaaag tcgattgaat cccgccatgg
       61 cagagccaca tgtcgccatc gtgggcggag ggattgtcgg cctggccacg gcctggcagc
      121 ttcaaaaccg ctatccgcgg attcgcgtca cggtgatcga aaaggaagcc gaagtcggcg
      181 agcaccagac cgggcataat tccggcgtgc tccactccgg catctactac aagcccggca
      241 cgctgcgcgc cgccaactgc cgcaccggca agcgggcgat ggaggagttc tgcgctgagc
      301 atggtgtgcc ctacgacatc tgcggcaaag tgatcgtcgc catcagcgag ccggagctgc
      361 cgctgctgga gcgtatcttc gagcgcggcc agcagaactg cgtccgctgc gagatgatcg
      421 ggccccagcg gctcaaggag ctggagccgc acacgccggg tatccgggcg atccacgtgc
      481 cggaagcggg aatcgtgaac ttccgcgagg tcgcccggaa gctggcggag ctagtcaaaa
      541 tgcgtggcgg cgaagtgcgc tgcaatgccc gcgtcatcgc cgtgcatcac gatgagggcg
      601 cgatcaccgt cgaatcaacg gccggcgagg ttcaggcgga ctatctcgtc acctgcgccg
      661 gcctgcagag cgaccgcgtg gcgaagctca ccggcgctca gccggaggca aaggtgctgc
      721 cgtttcgcgg cgagtactac gacctcaagc cggaggctca gcacctttgc cgcaatctga
      781 tctacccggt gccggatccg aatttcccgt tccttggcgt tcacttcacg cgcatgatcg
      841 aggggggcgt cgaatgcggc cccaacgccg tcctcgcctt cgctcgcgaa ggctatcgca
      901 agaccgactt caactgccgc gatttcagcg aggccctcgc ctatcgcggc tttcgcaagc
      961 tggcgtggaa gtattggcgc acgggcgcag gcgagatgtg gcgatcctgg agcaaagccg
     1021 cgttcgttaa ggcgctgcag cgcttgatcc ccgaaatccg aagcgagcac ctcgttgccg
     1081 gccgcagcgg tgtccgcgcc caggccattt cgcccgcagg atcaatcgtc gatgacttcg
     1141 tcatcgaggc ccgcggccag atcgtcaacg tcctcaacgc cccttcgcca gccgcaactt
     1201 cgtcgctgaa catcggcaag ttgatcgtgg agaaactggc cgagcagttg acagttcgtt
     1261 agcatgaatg cggagacaga gttgtgtgga gattggccat gagcattgcc tctgaagctc
     1321 gcgtgatctg ggacgccgag atgctcgccc agcgtttcgg gcccataccc ctggatcgca
     1381 tctgcggcga gcccgcgccg ggactgggga ccgaggacga tgtgattcga ttcaatgagc
     1441 gtgagaatcg cctgttcgag ctcgtcgatg gcgtactggt ggagaagacc atgggtgcgt
     1501 acgaatcgat gttggcggtg gaaatcgcaa gcttgctgcg gaactgggtc aagccgcgaa
     1561 agctggggac cgtccttggt tcggacggca ttctgcggct gtttgcgggg ctgatccgca
     1621 ttcccgatgt cgccttcctc agcatggaga aattcccgcg aggccgcttt cctcgcgata
     1681 aggccccgcc gctggccccc gacctggccg tggaggtcct cagcgaatcc aacaccaaga
     1741 aggaaatggc cgagaagctg cgggactatt tcgccgccgg ctcgaagctg gtgtggtacg
     1801 tcgatcccga gcgccgcgaa ttcacagttt ttacctctcc caccgacagc cgcgtcgtca
     1861 ccgagcaagg cacggtcgat ggcggccagg tgctgccggg cttcacgatg agcgtggcgg
     1921 agttgtttgc cgaactgccg ctggaagatg aggaaagttg agcgtgtcgg agcgcagcaa
     1981 gattcattcc tcacccgggt tggcctgatc aagcatttca agaaactcct gtttcacggc
     2041 gttgcgcgtt cgccattgag tcaccatccg attcacgtcg cctccttgaa gcgggaactg
     2101 attgaagcct tcgtcatccg gggaccagtc tagacctttg tgttgcgcca attgtcgaag
     2161 tgtcacaggc cgccttatga cgatggtccg aacttcgtcg ccttcgccca catgttcggt
     2221 gtcttccaaa taggccaccg ctttaatgtc agcaccgtgc tcaatgagtg ttctcgtgat
     2281 cgccacttcg cgtgatccat cgccgcgcag gccgaaagtg attgtccggt gtagttccat
     2341 cgcatcatgg agtggtgtcc ggtccagccg atccttggcg ttggcgctca gcctttgctt
     2401 gagcaggtat tcgactacct ccaggcgccc ttcgctcgcc gcgaaatgta gcgcggactg
     2461 gccgtcggcg ttcgttgcgt gcagattcgc cccatgcttg accaattgct cgatgattgc
     2521 cagttgtgcc gccgcgtcgg cttcgccctc tcctgccgca gccgcccaga gtagtggcgt
     2581 tatgccgctc ttgctggccg cgcgggcgtc ggccccagcg gcgagcaaga ggtcaaccgc
     2641 caggggtgag agcgcggcgc acgcgctgtg gagtgccgtg tactcttctt cgtcgcgggc
     2701 gttcacttca gcgccttcgt ggagcagcag tttgacgata cgactgtcac cgctggccgc
     2761 agcttcgtga agcggtgtcc tctgccagtc gttctttgcg gcgatatcag ctccatgaga
     2821 aaggagatac tgcgctacgc gcagtcgctt ccaccggcaa gcatggtgaa gcggcgatcc
     2881 gcgccagtca ttatcctggt cgttcacccg ggcaccgtgg gccacaagga gcctagctat
     2941 ctccaacgtc cgagcatgat gaattggccg ctcctcccaa gcgccgcgac cattagcgtc
     3001 tgcacccgct gccagcaaga gccggcccat cttcgcgctc tgcagcatgt gcaggggccg
     3061 gtcgcgcgtg gcgcattcgg aatttggatc ggctccgcgc gacagtagca gcctggccag
     3121 ttggcaatcg tccgccacaa ccgcacagtg cagcacggtc catccgtgga ttccgctgaa
     3181 atggtacgga tagctcgcat tcacaagctc agggtggagt ctcagcagac gatccagttc
     3241 gcggcaatcc cccagttggg ctgcggtgaa cagccgttgg atctggcgat ctcgcagttc
     3301 cttgtctagg tcgggcactt gtgctttgac cgtctgcaac gtgaacagga ttagcggaaa
     3361 aagggcaagg ccgggaaccg cgcgagtcgt gaacatttcg tgaccctcca agagccgtat
     3421 aaggtaatga acgacagcat c
//
LOCUS       DASUGB010001170         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_686776, whole genome shotgun sequence.
ACCESSION   DASUGB010001170 DASUGB010000000
VERSION     DASUGB010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686776"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFV87_22185"
     CDS             complement(<1..1472)
                     /locus_tag="VFV87_22185"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5106552.1"
                     /translation="MNPHPVGIDLGTTHSAIARLDQTGRTVMVPNVDGLFLTPSVVLF
                     DDAGIVVGREALKAIPVAAGRIARWVKRDMGSPSYSQPIGGHFLPPEAIQACILGQLR
                     TDIERQLGTAYAPVITVPAFFDEPRRRATAEAAAMAGIPLLDIVNEPTAAALAFGEQL
                     GYLDATGDAAEPLKLLVYDLGGGTFDVTIVDLRPGQIRALATDGDSMLGGYDWDRRLA
                     DFAAEEFLRTYGTDPRQDPLGLHQLLLSAEQAKLALSARQHATIRVTHNGKTREVPMT
                     RESFEERTSDLLYRTSMTSRQVLASAGLRWSDLDRVLLVGGSTRMPMVRRMLLDLTGR
                     EPHGEVNPDEAVARGAAIYAGHLLRKRQPAAPAAAVSGAAPQPRQLEVTNVSAHSLGI
                     DGYNVSLARRCNTVLIPRNSPLPARISRKFVTRKIGQHSIVVQVLEGESSRPEDCTRV
                     GRLVIRNLPPGLPAGTPVKVTYEYGANGRLNVTAQLSGSN"
     gene            complement(1503..4823)
                     /locus_tag="VFV87_22190"
     CDS             complement(1503..4823)
                     /locus_tag="VFV87_22190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5106553.1"
                     /translation="MHGLPLQPHPRYEILGLIGSGDFAAVYRARDRELGREVAVKQIH
                     AQYLSDPRRLERFWREAQLLASLEHPHIMTIYDIVRPNGWLVLELMQGTLLDATRGQP
                     IDLNLLRTTLISSLQALTLLHSRGTLHGDLKPSNLLVDKLGRIKLGDFGLARRVASDQ
                     GSYLKGATRYMAPETVAPQFGPVGPASDLYSLGFTAYELMCGTQQFELLFPGLEAFGR
                     DKQVAWMMWHAAADRRLPPVGTVLGGVPDDLARVIERLTIKDQTQRYRSAEQALADLR
                     PAGEVVAAVDQADPEEEAAKKRAAFHRRAVAVMALAASVLVSVLVLVYPTGGKPPPAP
                     PPDEVVRGVVRNVLPERDTLIIEDSATGSPREFRLEADDVVLLNEQTGLLRDLRQLDQ
                     VAVRIQHDLQGGEQLQVLVSRPKGDEGTIAEITPDEGTLSIALPETEQPLIVQVGAAC
                     TIELNGSAKQNGQPLSLADLRSGDRVTVQHYRDENGQTALSIAALRIVGGQGVVRHID
                     AKKREITLAAGTEDSAQTVVLPVAERCEVTLNGRRVLDGRLLTTADLKPGDAVTFQRD
                     VRLVSIAAQREFQAGGTISAINYDVRSFVASDGSMDRTYLLDPTCRILLGGVNAALED
                     LRRGDVVSVTFDSPDAQSPQVTSISADRPPDPGKWAIVVLGTNFDDATVAGYPAASAA
                     AARLRQTLAGRYAVPDEQLIVLDDPSRIRLEQAFPEAVSQAVSAEQLFVVFACRAVVD
                     PDGAPQIAPKDFAAARPAETSVPLVPLLSEVERSPAKEKIVLLDLQSAVASGPAKSPP
                     AADAFRSIQGTRSRPLLRATNVFAAKAAPSADASAPAEVAVSLAAGLAGAADPNRDNR
                     CSASELNEFFVASASGGGPQIELFLPNSTPPRITDDAREALRRLAALLGQTKIDPGEA
                     RVMFLAASKLAPQEPEPKLLSALVQLQARQYDEALAQFREIVVARPATPLAWEASAWA
                     KFEKRAYADGLRDLVWLLQHLPEDVPDDMAARIFPWIGRLREFSALALPADKRPNSAI
                     ALLDAEMLKRGVAESKLFDEGRAKTRLILAEMDQRIATATEPATINQLKYERWQLRHH
                     ASFSCERAAQEVLAHLDD"
BASE COUNT          793 a         1677 c         1561 g          805 t
ORIGIN      
        1 tcattcgagc ccgaaagctg cgcagtcacg ttcagccggc cgttggcgcc gtattcatag
       61 gtcaccttca cgggcgttcc ggccggcagc cccggcggca aattgcggat caccaggcgg
      121 ccgacgcgag tacaatcctc cggtcggctg ctctcgcctt ccagcacctg caccacgatt
      181 gagtgctgcc cgatcttgcg ggtgacgaac ttgcggctga tgcgggccgg cagcggcgaa
      241 ttgcgcggaa tcagcaccgt attgcaccgc cgagcgagcg atacgttgta gccgtcgatc
      301 cccaggctat gagcactgac gttggtcacc tccaattgcc gcggctgcgg cgcagcgcca
      361 gacaccgcgg ccgccggagc cgccggctgc cgcttcctca gaaggtgccc ggcatagatg
      421 gccgccccgc gggccaccgc ttcgtcggga ttcacctcgc cgtgcggctc gcggcccgtc
      481 agatcgagca gcatccgccg gaccatcggc atccgcgtcg atccgccgac gagcagcacg
      541 cgatcgagat cgctccaccg cagcccggcc gaagccagca cctgccgcga ggtcatcgac
      601 gtgcgataga gcaaatcgct tgtccgctct tcgaacgatt cgcgggtcat tgggacttcg
      661 cgcgttttcc cattgtgcgt gacgcgaatc gtcgcgtgct ggcgggcaga cagagcgagc
      721 ttggcctgct ccgctgaaag caagagctgg tgcaagccaa gcgggtcttg ccgcggatcg
      781 gtgccgtagg tccgcaggaa ctcctcagcg gcgaaatcgg ccagccggcg gtcccagtcg
      841 tatccaccca gcatcgaatc gccgtcggtt gccagggccc ggatctgccc cggccgcaga
      901 tcgacgatcg tcacgtcgaa cgtgccgcct cccaggtcat agaccagcag tttgagcggc
      961 tcagcggcgt cgccggtcgc gtcgagatag ccgagctgct cgccaaaggc cagcgccgcc
     1021 gcggtcggct cgttgacgat atcgagcagc ggaatccctg ccatcgccgc cgcctcggcg
     1081 gtcgcccgcc gccgcggctc atcgaaaaag gccggcacgg tgatcaccgg cgcataagcc
     1141 gtgcccaatt ggcgttcgat atcggtccgc aattgcccca ggatgcaggc ctgaatcgcc
     1201 tccggcggca gaaagtggcc gccgatcggc tgcgaatacg acgggctccc catatcccgc
     1261 ttgacccagc gggcgatgcg gcccgccgcc accggaatgg ccttcagcgc ctcgcggccg
     1321 acgacgatcc ccgcatcgtc gaacagcacg acgctcggcg tgaggaacag cccatcgacg
     1381 ttcggcacca tcacggtgcg gcccgtctgg tcgagccgcg cgatggccga atgtgtcgtg
     1441 ccgagatcga tgccaaccgg atgcggattc atagtagccg gcacactccg tgtgccgtgg
     1501 tatcagtcgt caaggtgcgc caaaacttcc tgcgctgctc gttcgcacga aaacgaagcg
     1561 tgatgccgca gttgccagcg ttcatacttg agctggttta ttgttgctgg ttccgtagcg
     1621 gtcgcaattc tttgatccat ctcagccagt atgagccgcg tcttcgcgcg gccttcgtcg
     1681 aacagcttag actccgcaac gccgcgcttg agcatctctg cgtcgagcag tgcaatggca
     1741 gaattcggcc tcttgtcggc gggcaatgcg agggccgaaa attctcgcag tcgcccaatc
     1801 cacggaaata tgcgggcggc catatcgtca ggaacgtcct ccggcaggtg ctggaggagc
     1861 caaacgagat cccgcaatcc atcggcatag gcccgcttct cgaacttcgc ccaggcgctg
     1921 gcctcccacg ccagcggcgt cgccggccgc gccacgacga tttcgcggaa ctgggccagc
     1981 gcctcgtcat actgcctggc ctgaagctgg acgagcgccg aaagcagttt cggctctggc
     2041 tcctgcggcg cgagcttgct cgccgcgagg aacatgacgc gggcctcgcc tggatcgatc
     2101 ttggtctggc ccaagagcgc cgccaagcgg cggagcgcct cccgggcgtc gtcggtgatc
     2161 cgcggcggcg tgctgttagg caggaacagc tcgatctgcg gcccgccgcc gctggcggaa
     2221 gcaacgaaga attcgttcag ctcgctggca gagcagcggt tgtcacgatt cggatcggcc
     2281 gctccagcca atccggcagc cagcgacacg gccacctcgg ccggcgccga tgcatcggcc
     2341 gagggcgcag ccttcgcggc aaaaacattc gtcgcccgca agagcggcct ggagcgcgtg
     2401 ccctggatcg accggaaggc gtcggctgcc ggcggcgatt tcgccgggcc ggaggcaacg
     2461 gccgactgca ggtcgagcag cacgatcttc tctttcgccg gcgagcgctc gacttcgctc
     2521 aatagcggca cgagcggcac gctcgtttcc gccgggcggg ccgcggcaaa gtccttcggc
     2581 gcgatctgcg gcgcgccgtc cggatcgacc actgcgcggc aagcgaacac gacgaagagc
     2641 tgctcggctg atactgcctg cgacaccgct tccggaaaag cctgctccag gcgaatgcgg
     2701 ctgggatcgt ccagaacgat gagctgctca tcgggcacgg cgtagcgccc agccagcgtc
     2761 tgccgcagcc tggccgccgc agcgcttgcc gcgggatagc ccgcgacggt cgcgtcgtcg
     2821 aagttcgtgc ccaggacaac gatcgcccac ttccctggat cgggcggccg atcggcggaa
     2881 atgctcgtga cttgcgggct ttgtgcgtcg ggcgaatcga acgtcacgct caccacgtcg
     2941 ccgcgccgca ggtcctccag cgccgcgttc acgccgccca gcaaaatgcg gcacgtcgga
     3001 tcgagcaaat acgtgcggtc catgctgcca tcactggcca cgaacgaccg cacgtcgtag
     3061 ttgatcgcgc taatcgtgcc gccggcctga aactcgcgct gggcggcaat gctcaccagc
     3121 cgcacatcgc gctgaaacgt caccgcgtcc cccggcttga gatcggccgt cgtcaagagc
     3181 cgcccgtcga gcacgcggcg gccgttgagc gtcacttcgc accgctcggc gaccggcagg
     3241 acgaccgtct gtgcggagtc ttcggtcccg gcggccagcg tgatctcccg tttcttggcg
     3301 tcgatgtgcc gcacgacccc ctggccgccg acgatccgca gcgccgcgat cgacagcgcc
     3361 gtctgaccat tctcgtcgcg atagtgctgc accgtcacgc ggtcgcccga acgcaaatcg
     3421 gccagcgaca gcggctggcc gttttgcttg gccgagccgt tcagttcgat cgtacacgcc
     3481 gcgccgactt gcacgatcag cggctgttcg gtctcgggca gggcgatcga cagcgttcct
     3541 tcgtcgggcg tgatttcggc gatcgtccct tcgtccccct tgggccgcga aacgagcacc
     3601 tgcagttgct cgcccccttg caggtcgtgc tgaatgcgca ccgccacctg gtcgagctgc
     3661 cgcagatcgc gcagcaaacc cgtttgctcg ttcagcagca ccacgtcatc ggcttccagg
     3721 cggaattcgc gcgggctgcc ggtggcggaa tcttcgatga tcagcgtgtc gcgctccggc
     3781 agcacgtttc gcacgacacc gcgcacgact tcgtccggtg gcggcgcggg cggcggcttg
     3841 ccgccggtgg gatagaccaa aaccagcacg ctcaccagca cactggcggc cagcgccatc
     3901 acggccacgg cccggcgatg aaatgcggcc cgcttcttgg cggcttcctc ttccgggtcg
     3961 gcctgatcga cggccgcgac gacttcgccc gccggccgca ggtcggccag ggcctgttcg
     4021 gccgagcgat agcgctgcgt ctggtctttg atcgtcagcc gctcgatcac ccgcgccaga
     4081 tcgtcgggca cgcctcccag caccgtcccc accggcggca ggcggcggtc ggcggcggcg
     4141 tgccacatca tccaggccac ttgcttgtcg cggccaaagg cttccagccc cggaaagagc
     4201 agctcgaatt gctgcgtgcc gcacatcagc tcgtaagcgg tgaagcccag cgaatacaaa
     4261 tcgctggccg ggccgacggg accgaactgc ggggcgaccg tttccggcgc catgtaccgc
     4321 gtggcccctt tcaggtagct cccctggtcg ctggcgacgc ggcgggccag gccgaaatcg
     4381 cccagcttga tccgccccag cttatcgacc agcaaattgc tcggcttcag gtcgccgtgc
     4441 agcgtgccgc gactgtgcag cagcgtcagg gcctgcagcg acgaaatcag cgtcgtccgc
     4501 agcaggttca ggtcgatcgg ctggccgcgg gtggcatcca gcagcgttcc ctgcatcagc
     4561 tccagcacca gccagccgtt gggccggacg atgtcgtaga tcgtcatgat gtgcggatgc
     4621 tcgagcgagg ccaggagctg ggcctcgcgc cagaagcgtt cgagccgccg cggatcgctc
     4681 agatactggg cgtggatctg cttgacggcg acttcgcgcc ccagctcgcg atcgcgggcg
     4741 cggtacacgg cggcgaaatc tccgctgccg atcaggccga ggatttcgta gcgcggatgg
     4801 ggctgaagtg gcaaaccgtg catcttggtt tgggaa
//
LOCUS       DASUGB010001171         9749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_686870, whole genome shotgun sequence.
ACCESSION   DASUGB010001171 DASUGB010000000
VERSION     DASUGB010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686870"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFV87_22195"
     CDS             complement(<1..791)
                     /locus_tag="VFV87_22195"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5106554.1"
                     /translation="MSDHLSRRELLAYSATFGSAFLAAQGLTAMEEKVASGQEPVAKA
                     AANPAGKKYDMQKSINLWALPYPQKMTLKECLQLCKDAGFDGVEVNYALEGDLSPEAS
                     EAAIKELGQMARKIGIQISGVCSFLFWPYAFTHEDPDRRKKGVELAGQMIRAARLLET
                     DNLLIVPGSVYAPWIPDEPPVAHNVALERATAGVKSLIPEAAKHKVHLNMENIFANGF
                     LHSPQEMNAFVDQFQSEWVKVHFDTGNIMQYHFPEHWIPQLGSRI"
     gene            complement(974..2116)
                     /gene="hemW"
                     /locus_tag="VFV87_22200"
     CDS             complement(974..2116)
                     /gene="hemW"
                     /locus_tag="VFV87_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5106555.1"
                     /translation="MSTDQQPRAAYIHVPFCRHRCGYCNFTLVAGRDDLIGAYLDALE
                     RELSWLRQPHEVDTLFFGGGTPTHLAPRELERLLANVRARFPVAAGGEFSIEANPTDL
                     NETKAAILAAASVTRVSLGVQSFDERKLAQLERDHLRMDIARAVDLARTFAQSVSLDL
                     IFGVPGENLNVWREDLHLAIHVIQADHVSTYGLTFEKGTAFWSRLKHGELARVDEDLE
                     RQMYELAIDTLTAAGYEHYEVSNFARPGHRCRHNENYWLGGEYFAAGPGAARFIGGRR
                     ETNHRSTTTYIRRVLAGQSPVAESEQLGPEDAARERLVFALRRLEGIDTAAFAAATGF
                     SIEQLGGDALPRLVDRGLLSLAANRLQLTRAGLLISDAIWPQFLRV"
     gene            2235..5081
                     /locus_tag="VFV87_22205"
     CDS             2235..5081
                     /locus_tag="VFV87_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106556.1"
                     /translation="MARVRYFLFSSFTLLPVVALAGDTSQPFASHQPARPLPMASERP
                     LEEGPAWFVHPAEGNDEWEGTRERPWQTMGHAVTRLQAGQTLVLRGGTYYEHVAARLV
                     GTIDKPITIRAFPGELAILDGGLREFFEQPDEAWEPCPGGAEGEFQSKKTYPELKAEG
                     DGPGVFGNFGDSLVPLQAYRFHGDLRSDNPFWNVENKVGSESFVYCGPGIFYNAETGR
                     IHCRLAHTKLSGLGDDNYRGETDPRKISLIVAGGEEPPLALVSCQNVRLQDLVLRGSR
                     RPTLQIHDCRNIELDGLSAYGGHSAVQVRDTWGLRMQHCACRGIGAPWTFRGMLKYRS
                     VEARIFDASHWDPTGADNRDFEIRYCEFTDSVDGVFLGNVRGVRFHHNLVENVTDDGV
                     FLTAGTAYDGQTWGGDMQFYQNRFARILTTFAFGVGHGRQKAIDLGDGKTGKQTGSGA
                     YIYRNVFDFRRPVWYTWPSGPDAPQEITSRGRFASDHGGPTWEPMWIYHNTILAGDTP
                     RYDYGCDGLIHGVASGAKRRVFNNIVVLTQGTPGSTLPPPEADFQADGNLFWSLAPDK
                     PLPENLFAKFQGSEAFERSKKEYPPGWTARDKTANPEFIELSADWHERPRLHPKLLSP
                     AYEGGVPLAKEWPDYGDRDGQQATIGALPMAPIPRRAGSPRIGVHGRVSLDDGAAIVN
                     WTGSWASADWHFKPSPSRDDATMPRALIVEGYPAFDAPIVAYLLRKVGWQVETSDRAW
                     ADTAKWKDYQLVVYDGSLARAGLEKTAFDEADVANVRAFLEAGGTLLLTRERHDLFRS
                     EAGRKLLAEIVGEGQREAKPQISILQPDHPWLAPLKTPIQRAAALPVFRFPDEKDKAP
                     PPAPALDPFPWLAKGTPIPTSKGQSLIGSPGGASLLHRAPFGRGQLIYVGWSPAASIP
                     HGREKSTLEMEADFEQQVAVLREIVESAQRGKAE"
     gene            5161..6606
                     /locus_tag="VFV87_22210"
     CDS             5161..6606
                     /locus_tag="VFV87_22210"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HEX5106557.1"
                     /translation="MDRRHFLATSAAAVSSAALANRPSIAADEPAACPCDRTFASPAE
                     AIKSPREELVFVVGVYAGTKTKAPDFLATVDLDPASPTYSQVIHRLPMPNVGDELHHF
                     GWNACGSCHGERQRQYLVVPGLVSSRIHIIDTADPRQPKLHKVIEPKEVIEKTGLTAP
                     HTVHCLASGEIMISMLGNADLGGPGGFLLLDQDFKITGHWAASRAGMHYNYDFWYQPR
                     HNVMISSEWAAPSTTRPGFKLEDVKAGKYGQHIHFWDWEARKIVQSVDLGEKGLIPLE
                     VRFHHNPDSTHGFVGAALSSVMWHWHKNGDKDGDKWQIDPVIEVEPREVAGWPFPVPG
                     LITDLLISMDDKWLYFSNWLHGDVRQYDVSDPARPKLTGQLVLGGVEGKGPSVRGRKL
                     TGGPQMLQLSLDGKRLYVTDSLFSSWDNQFYKAIGEQGSTMLQIDCDTERGGLSLNEK
                     FLVDFGTSPAGPARAHEMRFPRGDSTSDIWG"
     gene            complement(6685..8094)
                     /locus_tag="VFV87_22215"
     CDS             complement(6685..8094)
                     /locus_tag="VFV87_22215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5106558.1"
                     /translation="MFQDNKRHLPDDNPYASPQAVEQGPRLDEIAALAAASRPRGPSY
                     ARWVVLFFLCTLALVLYVDRVCIGQAEKSMREDLGLTKEHMGWIFNAFMIAYCVLEVP
                     TGHWGDRFGSRRVITRIVLWWSAFTALTGLAFGFWPLMAIRFLFGAGEAGAFPNAARV
                     VTRWFPDDERGFARGAITTTSQIGGAIAPPLAAFLIGLVDWRLTFMSFGALGLVWAFF
                     FYRWFRDEPGEHPAVSAAELAHIGKPPAHDDEHPSIPWGQVVLSPNVWLMGAIQTVGA
                     TLFYMLFQWYPTYLKEARQLSEQTSGWFTMIALSGGAVGCLMGGFLSDQIIRRTSERR
                     WTRRATGCAMLGLAAVSMWGVRYVEHPLTASVLCASALFCLQVSIPTWWTVVAEISGR
                     HGAAMFGLMNGMGGLGVMVLNALVGRIVDSRVAAGIPLADTWRPVFDGVAIALAVGAS
                     CWLLVDATRPIFRRQEGAA"
     gene            8243..9088
                     /locus_tag="VFV87_22220"
     CDS             8243..9088
                     /locus_tag="VFV87_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5106559.1"
                     /translation="MIIDVHSHAWSFPEHFSDDFRQQARRAKAGAEIDLSVRFEDYAA
                     RASEPVTTIVFGGKARLSGLWVDDRYVADYVAARPDRLIGFLALDPTQPGWEEELRFG
                     HQELKLRGIKLMPMYAGFRPDEPRLDALWGYAQRHHLPVLLHTGTTFVSQAPLECTLP
                     RHLDPVAARFPEVPIIMAHLGHPYEGECVVTARKHSHLHADISALHYRPFQLYHSLML
                     VQEYGIWDKVLFGTDYPFTNVDATLAGLRGLNEMLAGTALPLLDEDAIERMIYRDTLP
                     LLGLE"
     gene            9246..>9749
                     /locus_tag="VFV87_22225"
     CDS             9246..>9749
                     /locus_tag="VFV87_22225"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106560.1"
                     /translation="MRITSVEPILLRGEQSYQATQGSAEAVDNGDWQLLVKVTTDEGL
                     VGWSDVETLAAAAWACINGPGMSILGFRCLKDLLIGEDPLADPQALWDKLYIGTSYYG
                     RRGVALHCISAIDNCLWSIRAQAAGVSLGELLHRSPLAPREEMEPPLSRMVAPDSGLD
                     RDSRSESP"
BASE COUNT         1794 a         3176 c         3096 g         1683 t
ORIGIN      
        1 tgaatccgtg agccgagctg cggaatccag tgctcgggga agtggtactg catgatgttg
       61 ccggtgtcga agtggacctt gacccactcg ctctggaact gatcgacgaa ggcgttcatc
      121 tcctgagggc tgtgcaaaaa gccgttggca aaaatgttct ccatgttgag atggactttg
      181 tgcttcgccg cttccgggat caggcttttc acgccggcgg tcgcgcgctc caaggccacg
      241 ttgtgggcga cgggcggctc gtcgggaatc cagggggcat agacgctgcc ggggacgatc
      301 agcaggttgt cggtttccaa gaggcgcgcc gcgcggatca tctggccggc tagctccacc
      361 cccttctttc gccggtcggg atcttcgtgc gtgaaggcgt acggccagaa caggaacgag
      421 cagacgccgc tgatctggat gccaatcttg cgggccatct gccccagttc ctttattgcc
      481 gcttcgctgg cctccggcga caagtccccc tccagggcgt aattcacttc cacgccgtcg
      541 aaccctgcat ccttgcagag ctgcaagcac tccttgagcg tcatcttctg cgggtagggc
      601 agcgcccaga ggttgatcga cttctgcatg tcatacttct tgccagccgg attcgccgcc
      661 gccttggcca ccggctcctg gccactggct actttctctt ccatcgcagt tagaccctga
      721 gcggcgagaa atgcggagcc gaacgtggcg gaataggcaa gcaattcgcg gcgggagagg
      781 tggtcggaca ttggtgatag ctcctgagtt cgtcgatgaa cagctaacga caatatctca
      841 cacagcggct cagaggcaca gtgaagccaa ccaaatcggt tgccgtcgca atcagccttt
      901 tcactcgtgc gcactgctgt ttccagtatc tccgcgacgc tgcacctctg tgcctctgtg
      961 tgagattcag gtgctatacc cgcaggaact gcggccagat ggcgtcgctg atgagcaggc
     1021 ccgcgcgggt gagctggagc cgattggcgg cgagtgacaa cagtccgcgg tcaacgagcc
     1081 gcggcagggc gtcgccaccg agctgttcga tcgagaagcc agttgctgcg gcaaacgcgg
     1141 cggtgtcgat tccttccagc cgccgcaggg cgaagacgag gcgttcgcgg gcggcgtctt
     1201 cggggccgag ctgctcggat tcggcgacgg gcgattggcc ggcgagcaca cgacggatgt
     1261 aggtggttgt gctgcggtga ttggtttcgc gccggccgcc gatgaaccgc gctgcgccgg
     1321 ggccggcggc gaaatactcc ccgccgagcc agtaattttc gttatgtcgg cagcgatggc
     1381 cgggacgggc gaaattcgag acctcgtaat gctcgtatcc ggctgcagtc agcgtgtcga
     1441 tcgccagctc gtacatctgg cgttcgaggt cctcgtccac gcgcgccaac tcgccgtgct
     1501 tcagccgtga ccagaaggcg gtcccctttt cgaacgtcaa tccgtacgtg gaaacgtggt
     1561 cggcctgtat cacgtgaatg gccaggtgca ggtcttcgcg ccagacattc aggttctcgc
     1621 cggggacgcc gaaaatcagg tccagcgaga cagattgggc gaacgtgcgt gccagatcga
     1681 cggcccgcgc gatgtccatt cgcagatggt cccgctcgag ttgggccagc ttccgctcgt
     1741 cgaatgactg gacgccgagg ctgacgcgcg tcacgctggc cgcggccaga atcgccgctt
     1801 tggtctcatt cagatcggtc ggattggcct cgatgctgaa ctcgcccccc gccgccaccg
     1861 gaaaccgcgc ccgcacattc gccagcagcc gctccagctc gcgcggggcc aggtgcgtcg
     1921 gcgttccgcc gccaaagaag agcgtatcga cctcgtgcgg ctggcggagc cacgacagct
     1981 cccgctccaa agcgtcgaga tacgctccga tcaaatcgtc ccgcccggcc accagcgtga
     2041 agttgcaata cccgcagcgg tggcggcaga agggaacgtg aatatacgcc gcgcggggct
     2101 gctgatcggt gctcattccg caacgattct atcccactgc atggatggga gaagatgcga
     2161 gtacaatcaa ccttgctggg catcccgtgg tcggtacatg gcgaacgtac gccgaccaat
     2221 gcaggagaga aatgatggcg cgagttcggt actttctgtt tagcagcttc acgctgttgc
     2281 ccgtggtggc tttggccggt gatacttcgc agcccttcgc ctcgcaccag ccggctcggc
     2341 cgctgccgat ggcgagcgag cggccgctgg aggaagggcc ggcctggttt gtgcatccgg
     2401 cggaggggaa cgacgaatgg gaagggacca gggagcggcc gtggcagacg atgggccacg
     2461 cggtaacgcg tctgcaggcg ggccagacgt tggtgctgcg cggcgggacg tattacgagc
     2521 atgtcgctgc ccggctggtc ggcacgattg acaagccgat cacgattcgg gcctttccgg
     2581 gcgaattggc gattctggac ggcgggctgc gggaattctt cgagcagccg gacgaggcgt
     2641 gggagccctg ccctgggggc gcggagggcg aattccagtc gaagaagacc tatcccgaat
     2701 tgaaggcaga gggtgatggg ccgggagtct tcggcaactt tggcgattcg ctggtgccgc
     2761 tgcaggcgta tcgctttcat ggcgatttgc ggagcgacaa cccgttttgg aacgtcgaga
     2821 acaaggtcgg cagcgaaagc tttgtctatt gcgggccggg cattttctat aacgccgaga
     2881 ccgggcggat tcactgccgg ttggcgcaca cgaagctgtc tggcctgggc gacgacaatt
     2941 accgcggcga aaccgacccg cgcaagatct cgctcatcgt cgccggcggc gaagagccgc
     3001 cgttggcgct cgtcagctgc cagaacgtgc gcttgcagga cctggtgctt cgcggctcgc
     3061 ggcggccgac gctgcagatt cacgattgcc gcaatatcga gctggatggc ctgtcggctt
     3121 acggcggcca ttcggcggtg caggtccgcg atacctgggg cctgcggatg cagcactgcg
     3181 cctgccgcgg aatcggggcg ccgtggacgt tccgcgggat gctcaagtac cgcagcgtcg
     3241 aagcccggat cttcgacgcc agccactggg acccgaccgg cgccgacaac cgtgatttcg
     3301 aaatccgcta ttgtgaattc accgacagcg tcgatggcgt cttcctgggg aatgtccgcg
     3361 gcgtgcggtt ccaccacaat ctggtcgaga acgtgaccga cgacggcgtg tttctcacgg
     3421 cgggcacggc gtacgacggc cagacgtggg ggggcgatat gcagttctat cagaaccgct
     3481 tcgcccgcat cctcacgacg ttcgccttcg gcgtcggcca cgggcggcag aaggcgattg
     3541 atctgggcga cggcaagacc ggcaagcaga ccggcagcgg ggcgtacatc tatcgcaacg
     3601 tgttcgattt ccgccggccc gtctggtaca cgtggccgag cgggccagac gccccgcagg
     3661 aaatcactag ccgcggccgc ttcgccagcg atcacggcgg cccgacctgg gagccgatgt
     3721 ggatctatca caacacgatt cttgccggcg acacgccgcg gtacgactac ggctgcgacg
     3781 ggctgatcca cggcgtcgcg tccggcgcga agcggcgggt ctttaacaac attgtcgtcc
     3841 tcacgcaggg gacgcccggc agcacgctcc cgccccccga agccgatttt caagccgacg
     3901 gcaacctgtt ctggtcgctc gcgcccgaca agccgctgcc ggagaatctg ttcgcgaagt
     3961 tccaggggag cgaggcgttt gagcggagca agaaggagta tccgcctggg tggacggcgc
     4021 gtgataagac ggcaaatcca gagttcatag agctttctgc ggactggcat gaacgaccgc
     4081 gactgcaccc taagctcctc agcccagcgt atgagggagg cgtgccactg gcgaaggaat
     4141 ggcctgatta tggtgaccgc gacggacagc aagcgaccat tggcgccttg ccaatggcac
     4201 ccatcccccg cagggctggc agtccaagaa ttggtgttca tgggcgagtt tcgttagacg
     4261 acggcgcggc aattgtcaat tggacgggat cgtgggcctc agccgactgg cacttcaagc
     4321 cctctccttc ccgcgatgac gcaacgatgc cgcgggcgct gatcgtcgaa ggctaccccg
     4381 cgttcgacgc gccgattgtc gcgtatctgc tgcgcaaggt gggctggcag gtcgaaacga
     4441 gcgaccgcgc atgggcggac acggcgaagt ggaaggatta ccaactcgtc gtctatgacg
     4501 gcagcttggc gcgggccggg ctggagaaga cggcgtttga cgaggccgat gtggcgaacg
     4561 tgcgggcgtt tctcgaagcg ggcggcacgc tgctgctcac gcgcgagcga catgacctgt
     4621 tccgctctga ggccggccgc aagctgctcg ccgaaatcgt cggcgaaggc cagcgcgaag
     4681 ccaagccgca gatttccatt ctgcagccgg atcatccctg gctcgcgccg ttgaaaacgc
     4741 cgatccagcg cgccgccgcg ctccccgtgt tccgttttcc cgacgagaaa gacaaagcgc
     4801 cgccgccggc ccccgcgctc gaccccttcc cctggcttgc caaaggcacg cccatcccga
     4861 ccagcaaggg ccagtcgctc atcggcagcc ccggcggcgc gtcgctcctg caccgcgccc
     4921 ccttcggccg cgggcaattg atctatgtcg gctggtcgcc cgctgcctcg attccgcacg
     4981 gacgcgagaa atcgacactg gaaatggagg cggatttcga acagcaagtg gccgtgctgc
     5041 gcgagatagt ggagtctgca caacgtggca aagctgagta gggtgggctg tgcccaccgc
     5101 tgtacgattg gcatttcgat ggtgggcaca gcccaaccta cacgaatcgg agttcgatcc
     5161 atggaccgcc gccattttct cgccacttcc gccgcggctg tttcgtccgc cgcgctagcc
     5221 aatcgcccat cgatcgccgc ggatgagccc gccgcatgtc cctgcgaccg gacgtttgcc
     5281 tcgcccgcgg aggcgatcaa gtcacctcgt gaggaactgg tctttgtcgt cggcgtatat
     5341 gccggcacca agacgaaggc ccccgatttt ctggccacgg tcgatctcga tcccgccagc
     5401 ccgacgtatt cgcaggtcat ccatcgcctg ccaatgccga acgtcggcga cgaattgcac
     5461 cacttcggct ggaacgcctg cggaagctgc cacggcgagc gccagcggca gtatctggtc
     5521 gtgcccggcc tcgtcagcag ccgcattcac atcatcgaca cggctgatcc ccgccagccg
     5581 aagctgcaca aggtgatcga gccgaaagag gtgattgaaa agaccggcct gacggccccg
     5641 cacacggtcc attgcctggc gagcggcgag atcatgattt cgatgctggg caacgccgat
     5701 ctcggcggcc ccggcggttt cctgctgctc gaccaggatt tcaaaatcac cgggcactgg
     5761 gccgccagcc gcgccggcat gcactacaac tacgacttct ggtatcagcc gcggcacaac
     5821 gtaatgatct ccagcgagtg ggccgccccc agcaccacgc ggcccggctt caagctggaa
     5881 gacgtgaaag cggggaagta cggccagcac atccatttct gggattggga agcgcggaag
     5941 atcgtgcagt cggtcgatct gggcgaaaaa gggctgattc cactggaggt ccgctttcac
     6001 cacaaccccg acagcacgca cggtttcgtc ggcgcggccc tgtcgagcgt gatgtggcac
     6061 tggcataaga acggcgacaa agacggcgac aaatggcaga ttgacccagt catcgaagtc
     6121 gagccgcggg aagtcgccgg ctggccgttc ccggtgccgg ggctcattac cgatttgctg
     6181 atttcgatgg acgacaagtg gctgtacttt tcgaactggc tgcacggcga cgtgcggcag
     6241 tacgatgtga gcgacccggc caggcccaag ctcaccgggc agcttgtgct gggaggcgtc
     6301 gaagggaagg ggccgagcgt tcgcgggcga aagctcaccg gcggcccgca aatgctgcag
     6361 ctcagcctcg acggcaagcg cctctacgtg accgattcgc tcttcagcag ttgggacaac
     6421 cagttctaca aagcgatcgg cgagcaaggc tcgacgatgc tgcagatcga ctgcgacacc
     6481 gagcgcggcg gcctgtcgct caacgaaaaa ttcctcgtcg atttcggtac gtcccccgct
     6541 ggcccggccc gcgcccacga gatgcggttc ccccgcggcg attcgacgtc ggacatctgg
     6601 ggttgaaaat ctggcttacc cgaaagtcat cgcttccggg tggatgagcg ggggcgcctt
     6661 cacaacagaa gtgacgccgg ccactcaagc ggctccttcc tgcctccgga aaatcggccg
     6721 cgtggcatcg accaagagcc agcagctcgc gccgaccgcc agcgcgatcg ccacgccgtc
     6781 gaagacgggc cgccacgtat cggccagcgg aattccggct gccacgcggg aatcgacgat
     6841 ccggcccacc agcgcgttga gcaccatcac gcccagcccg cccatgccgt tcatcaagcc
     6901 aaacatcgcc gcgccgtgcc ggccgctgat ttcggccacg accgtccacc aggttggaat
     6961 cgacacctgc aggcagaaca gggcgctggc acacaggacc gaagccgtca gcggatgctc
     7021 gacgtaccgc acaccccaca tcgataccgc cgccaggccg agcatcgcgc agccggtcgc
     7081 ccgccgcgtc caccgccgct cgctggtgcg gcggatgatc tggtccgaca gaaagccccc
     7141 catcaagcag ccgaccgcgc cgccgctcaa ggcgatcatg gtgaaccagc cggaggtttg
     7201 ctccgagagc tggcgggctt ccttgaggta cgtgggatac cactggaaga gcatgtagaa
     7261 cagcgtcgcc ccgaccgtct gaatcgcgcc catcagccag acattggggc tcaagacgac
     7321 ctgcccccac ggaatcgatg ggtgctcgtc gtcgtgcgcc ggcggcttgc cgatgtgcgc
     7381 cagttcggcc gcgctcaccg caggatgctc gcccggctcg tcgcggaacc agcgatagaa
     7441 gaagaaggcc cacaccaggc ccagcgcccc gaagctcatg aacgtcagcc gccaatcgac
     7501 caggccaatc agaaacgcgg ccagaggcgg cgcgatcgcg ccgccgatct gtgaagtcgt
     7561 cgtgattgcg ccgcgggcaa agccccgctc gtcgtctgga aaccagcgag tcactacgcg
     7621 ggcggcgttg ggaaacgctc ccgcttcgcc cgctccgaac aaaaatcgaa tcgccatcag
     7681 cggccagaag ccgaacgcca gcccggtcag cgccgtgaac gccgaccacc agaggacgat
     7741 tcgcgtgatg acccgccgcg agccaaagcg gtcgccccag tggccggtag gaacctcgag
     7801 cacgcaatag gcgatcatga acgcgttgaa gatccagccc atgtgctcct tggtcaggcc
     7861 caggtcttcg cgcatcgact tctcggcctg gccgatgcac acgcgatcga cgtaaagcac
     7921 tagcgccagc gtgcagagga agaacagcac gacccagcga gcataacttg gtccgcgcgg
     7981 acgagaggcg gcggcaaggg cggcgatctc atccagccgc ggaccctgct ccaccgcctg
     8041 cggcgaggca tagggattgt catccggcag gtgccgcttg ttatcctgaa acatcgtggt
     8101 tcctagcata acgtagtcct ctcgctccga ccgtagtcct ctcgctccgc gagaggaaat
     8161 tcggcctcca tcttcgcgcg acgtcgcgcc aaatagcagc gtccgttgcg actcgcggag
     8221 cgagtcgcct acgattgagc ccatgatcat cgacgtccac agccacgcct ggtccttccc
     8281 ggagcacttc tcggacgact tccgccagca ggccaggcga gcgaaggccg gagccgagat
     8341 cgatctgtcc gtacggttcg aggactacgc cgctcgcgcc agcgagccgg tgacgaccat
     8401 cgtcttcggc ggcaaagcgc ggctgagcgg cttgtgggtc gatgaccggt atgtcgccga
     8461 ctacgtagcc gcccgtccgg accggctcat cggctttctc gcgctcgatc cgacgcagcc
     8521 cggctgggag gaagaactgc gcttcggcca tcaggagctc aagctccgcg gcatcaagtt
     8581 gatgccgatg tacgccggct tccgccccga tgagccgcgg ctggacgccc tctggggcta
     8641 cgcccaacgc caccacctgc cggtgctgct gcacaccggc accacgttcg tttcgcaggc
     8701 cccgctcgaa tgcacgctcc cgcggcatct cgacccggtc gctgcgcggt tccccgaggt
     8761 gccgatcatc atggcccacc tgggccatcc gtatgaaggc gagtgcgtcg tcaccgcccg
     8821 caagcattcg cacctccatg ccgacatcag cgccctccac tatcgcccgt ttcagctcta
     8881 tcacagcctg atgctcgtgc aggaatacgg catctgggac aaggtcctgt tcggcaccga
     8941 ttacccgttc acgaacgtcg atgccacgct tgccggcctc cgcggcctca acgaaatgct
     9001 cgccggcacc gccctcccgc tgctcgacga ggacgcgatc gaacggatga tctatcgcga
     9061 cacgctgccg ctattggggc tggagtgacg aatccaaccc gaagcgtcag cgagggagtc
     9121 accatggttc gccaggaagc acgattcacg agttaacgcc ctctctcgcg atccgaaacc
     9181 atatatccgt cgaaaccgcg cttacgcatc tatccaccga tacctcgctt acgcttcggg
     9241 ttggtatgcg catcacctcc gtcgagccga tcctcctccg cggcgagcag tcgtatcagg
     9301 ccacgcaggg cagcgccgaa gcggtcgata acggcgattg gcaactgctt gtcaaagtga
     9361 cgaccgacga agggctcgtc ggctggtcgg atgtcgagac gctggccgcg gcggcctggg
     9421 cctgcatcaa tggcccgggg atgagcatcc tcggctttcg ctgcctgaag gacctcttga
     9481 tcggcgaaga cccgctggcc gatccgcagg cgctgtggga caagctctac atcggcacca
     9541 gctattacgg ccgccgcggc gtcgccctgc actgcatctc cgcgatcgac aattgcctct
     9601 ggtcgatccg ggcccaggcc gccggcgtgt cgctgggcga gttgcttcac cgtagtcctc
     9661 tcgctccgcg agaggaaatg gagcctccac tctcgcgcat ggtcgcgcca gacagcggtc
     9721 tcgatcgcga ctcgcggagc gagtcgcct
//
LOCUS       DASUGB010001172         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_130080, whole genome shotgun sequence.
ACCESSION   DASUGB010001172 DASUGB010000000
VERSION     DASUGB010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130080"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2007
                     /gene="hrpB"
                     /locus_tag="VFV87_22230"
     CDS             <1..>2007
                     /gene="hrpB"
                     /locus_tag="VFV87_22230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX5106561.1"
                     /translation="VLRAPPGAGKTTRVPPAILAAGLEKQGKIVVLQPRRVAARATAA
                     RIASEHGWRLGEEVGYQVRFESRTGPQTRIEIVTEGILLRRLQGDPFLPDTSVVVFDE
                     FHERSLASDLALAMVRQVQQSVRPELRIVVMSATLAAEPIARYLGGSPVVESAGRVFP
                     VDVRHVPHLDRRPLVKQVTEGVEQILDLTSGDCLVFLPGVGEIRRCHDALERLAEARD
                     LALLELYGDLPAEQQDAVLAPGQRRKVILSTNVAETSLTIEGVTGVVDSGLARVLRYD
                     ERTGLDRLELSPISKASAEQRAGRAGRTQPGVCLRLWPAAAHRIRPEYELPEIQRVDL
                     AGAVLQVLCWIEPDVASFPWFEPPPAGAIERARQLLIKLGAADDAGITPLGRQIARLP
                     VHPRIGRLLIEGARMGVLEQVALAAALLTERDPFQRPERVSGQRRGPTERSRSDVLDR
                     IQALDQGSGSSSLGTLNRNAANHIFRTRDQLVGQVSSLSLPDGLESCPAAEALLRAIL
                     AAFPDRLARRREPGSRRGLMVGGRGVRLAEESALGDEQLYVCVDVDAGAGEALVRQAS
                     AVDRASLPEEQIITSTAVEFDESSGKVLAKKRVQFDTLVLEESQGVLPGADEIAQVLA
                     EAAASRWDRAFPADDPDLCSFISRVRCLAQWMPDLNFPSLDDAHL"
BASE COUNT          313 a          635 c          712 g          347 t
ORIGIN      
        1 gtcctccgcg ccccgccggg agcaggaaaa acgactcgcg ttccgcccgc gattctggcg
       61 gcagggctgg aaaagcaggg aaaaatcgtc gttctgcagc cccgccgtgt cgcggcgcgg
      121 gccaccgccg cccggatcgc atcggagcac ggctggcggc tgggagagga ggttggctat
      181 caggttcggt ttgaatcgcg gaccggcccg cagacgcgga tcgagatcgt cacggagggc
      241 atcctgctgc ggcggctgca gggcgaccca ttcctgcccg acacgtcggt cgttgtgttc
      301 gacgagttcc acgagcggtc gctggccagc gatctggcgc tggcgatggt ccgtcaggtg
      361 cagcagtcgg tccggccgga actgcggatc gtcgtcatgt cggcgactct cgccgccgag
      421 ccaatcgccc gctatctcgg cggatccccc gttgtcgaaa gcgcgggacg agtgtttccc
      481 gttgacgtcc gccatgtgcc gcacctggat cgccgtccgt tggtgaagca agtgacagag
      541 ggagtggagc agattctcga cctgacaagc ggcgactgtc tcgtatttct gcccggcgtc
      601 ggcgagattc gccgctgtca tgacgcgctc gagcgattgg ccgaagcacg cgatctggcg
      661 ctcctcgaac tttatggcga cttgccggcc gagcagcaag acgcggtcct cgcgccgggc
      721 cagcggcgca aagtcattct ctcgacgaac gtcgccgaga catcgctcac gatcgaaggg
      781 gtcaccggcg tcgtcgatag cgggctggcc cgcgtgctgc gctacgacga gcggaccggc
      841 ctcgaccgcc tggagctgtc gccgatctcc aaagcctctg ccgaacagcg cgccggccgt
      901 gcgggacgaa cgcagccggg cgtttgcctg cgcctgtggc cggcggcggc gcaccgtatt
      961 cggccagagt acgaactgcc ggagattcag cgcgtcgatc tggcaggcgc ggtcttgcag
     1021 gtgctgtgct ggattgaacc cgatgtcgcg agcttccctt ggttcgaacc gccgccggct
     1081 ggagcgatcg agcgggcgcg gcagctattg atcaagcttg gagcggctga tgacgccgga
     1141 ataacgccgc tcggcaggca gattgccagg ctgccagtgc atccgcggat tggccggctg
     1201 ctgatcgaag gggcccggat gggcgtgctg gaacaggtcg cgctggcggc ggcgctcttg
     1261 acggaacgtg acccgttcca gcggcccgag cgcgtgtctg gtcagcggcg cgggccgact
     1321 gagcgcagcc gcagtgacgt gctcgaccgg attcaggcgc ttgaccaggg cagcggtagc
     1381 agttcgctgg gtacgctcaa ccgcaatgct gcgaaccaca tctttcggac gcgcgaccag
     1441 cttgtaggac aggtttccag cctgtccctc ccggacggct tggaaagctg tccggcggcg
     1501 gaggcccttc tgcgggcgat tctggcggca tttcccgatc ggctggcgcg gcggcgcgag
     1561 ccgggcagtc gccgcggcct gatggtcggc gggcgcggcg tgcggctggc cgaggagagc
     1621 gctctggggg acgagcaact ctatgtctgc gtcgatgtgg atgccggagc gggcgaggcg
     1681 ctcgtccggc aggcctcggc tgtcgatcgg gcctcgttac cggaggagca gatcattacc
     1741 agcacggctg tcgaattcga cgaatcgagc ggcaaggtct tggccaaaaa gcgcgtccag
     1801 ttcgacacac tcgtgctgga ggaatcgcag ggggtgcttc cgggagccga cgaaatagcg
     1861 caggtcctgg ccgaagccgc cgccagccgc tgggatcggg cgtttccggc tgacgatccc
     1921 gacctttgct catttatcag ccgcgtccgc tgcctggcgc agtggatgcc cgatctgaat
     1981 tttccatcgc tcgacgatgc ccacctt
//
LOCUS       DASUGB010001173        12739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2077079, whole genome shotgun sequence.
ACCESSION   DASUGB010001173 DASUGB010000000
VERSION     DASUGB010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077079"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFV87_22235"
     CDS             complement(<1..636)
                     /locus_tag="VFV87_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5106562.1"
                     /translation="MSYAQERSPQSTEVARLHVRGKDLELPVVVGSEDEPGIDISKLL
                     ATTGCTTLDEGYANTGSTSSHITFLDGERGILRYRGYAIEDLAANCDFLEVAHLLIYG
                     ELPSEQELQTFRGTILRHTMLHEEMRKFYGGFPRDAHPMAILSAATSALSTFYQDSLD
                     PGDPRQVEVSIHRLLAKLPTIAAYSYKKSVGQPFVYPQNDLSYTANFLQMMF"
     gene            complement(775..2181)
                     /locus_tag="VFV87_22240"
     CDS             complement(775..2181)
                     /locus_tag="VFV87_22240"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5106563.1"
                     /translation="MTPRAARLATLLILALGVRAASGQQPDPNRYPITVQQPTFRFGQ
                     NPVPGAAQAPMPGPMQPATAAPTESLRYPLPEPAGPPSPLVTAEPRTPNTEPQLPIPK
                     GELFEPGRIIATVGDKFILYGDVSPIVNQLLQPMLDKATNPLEQHEIEKYREPLTRQT
                     LNQMIETKLMYLEFERDLLKKAPKDKLAEVQANVTKKVSESFENDLREMRKKVADAKP
                     DQIQELMRRDPNVTRLALLMRDNQVETLGELDLLLRRFGSTLEKQVRYYGEYKLGRTG
                     IATHINFNPEVTHQEMLDYYYEHAADYAVPAKARFEILTAKSANFPNRGAAYSAIAAM
                     GNEVFFGAPFKAVAQKSSQEPNAKQGGYYDWTSQGSLASEPINQALFTLEVGKLSQII
                     EDDQGYHIVRVIERTPAGQVSFLDAQKEIKKAIELRKRDGDYKKIVEKLSHGTQVWSI
                     YDSSTATAPPQPGAPVPR"
     gene            complement(2320..5952)
                     /gene="mfd"
                     /locus_tag="VFV87_22245"
     CDS             complement(2320..5952)
                     /gene="mfd"
                     /locus_tag="VFV87_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5106564.1"
                     /translation="MTAVLPHATASERMRELAPQLESYAGFSEALAELAAGRPASFDG
                     VWGSACALIAAGLADRRPLAPREEIDSGGGSTVAPPLLVICPTARDADDLAADLELFT
                     AVEPQLYPTWETTPDERLVHDETFGERLRVLKRLLNPAGPKLAGADASPVPTPRGLGN
                     FIKSRRNEPDPEEGARADHGTPQRAFPTALTPILVTSIQALLQPSTPKEAIAAATRQL
                     RKEERIDVETFLRWLVEHGFHHTTAVELPGEFSHRGGIIDIFAPDWPRPVRVELFDDE
                     IESLRTFEVGTQRSVETLDEIDVTVLRADTHLPLPLGEGRGEGAGTLLALKRPLPNPL
                     PEGEGTIAGHLTDYLPPGSQILLVDPERIGEEGKHYLQRVEKPAEFHSVNAVLAECGR
                     FAVATAGEIQSGTAGVHCHLPCESVERFSGDISRVRDELDSIAHEDEVFLVCPTEAEV
                     ERLREILATTRLAAAGRLHFPIGSLRAGFRLRPPVGNALRGVPGSGAANELAVTGTPR
                     RAFPTAPEGRGMLILTAGELFHRGELRRLPRRRLGKVIDSFLDLREGDLVVHLAHGIG
                     RYRGLKLIERQGQHEEHLEIEFDGGTKIYVPAVKIDLVQKYVGGTKTRPSLAKIGGKT
                     WLRQKKDAEAAVHDLAVEMLEMQAIRRSRPGISFGSESDWQREFDHSFPYHETADQLG
                     AISAIKADMQRPRPMDRLLCGDVGFGKTEVAMRAAFKAVDHGYQVAILVPTTILAEQH
                     FRSFSERMAEFPFDIAKLSRFCTADEERAAVAGLKSGKIDIVIGTHRVASKDIEFKNL
                     GLVIIDEEQRFGVDVKERLKMLRATVDVLTLSATPIPRTLHMSLVGLRDISNLETPPE
                     DRLAVETKVARWDNGLIRHAVLRELARGGQIYFVHNRVNDIELIANRLREIAPEAKLR
                     IGHAQMPDHELEEVMVDFVAGRFDLLLSTTIVESGLDIPNANTIFIDEADRYGLADLH
                     QLRGRVGRYKNRAYCYLLIEPHKHLTPNAARRLRAIEEFSEMGAGFAIAMRDLEIRGA
                     GNLLGTQQSGHIAAVGYELYCTLLENAVRALQQMPPKVRLEVDVNLPGDAFLPADYVP
                     DLRLKIDLYRRLARVAKYDDLTDFRSELVDRFGEPPQPVERLLLLAELKMDAASWQIS
                     TVFIENDFVVFRYDSRPRIEQLARQHKGKLRIVDDKSAYLPLPKELTETDRIWRITKS
                     LLRQPP"
     gene            6136..9111
                     /locus_tag="VFV87_22250"
     CDS             6136..9111
                     /locus_tag="VFV87_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5106565.1"
                     /translation="MRSFLLLAPVAAILLLMFAHSATAADPVPQWLWTNKEPKENESA
                     FFRKAIELDAAPKSATFYGACDNVMTLFVNGEQVTQHSAWDAATRENLTKRLKAGKNV
                     IAIRASNQGGPAAFIAQINIERADGTKLVIVTDESWKAASEARGNFRTERYDDSAWGK
                     PHSFGKLGIAPWGDIALTSATASGSAATPVEAIQTLPGFKVELLYSVPKESEGSWVNM
                     TSDNKGRLIVSDQYGALYRVMPGQDAETTQVEQLAVPLGEAQGLLYAFDALYVVVNRG
                     QSYESGLYRLTDTNGDDQFDKVEQLKKINGGGEHGPHAVKLGPDGKLYVIAGNHTQTP
                     DGLDPASPHRNWAEDLLLPRNPDGNGHATGVMAPGGWVCRTDKDGRYWEILCAGFRNE
                     FDIDFNTDGELFTYDADMEWDTGAPWYRPTRVNHCVSAGEYGWRYGTGKWPDYSADSL
                     GAVVDIGLGSPTGIVFGTGAKVPAKYQQALYINDWTYGKIYAVHMTPSGASYTGTFEA
                     FITGRPLPVTDVVVHTDGQLYFTIGGRRTQSGLYRVTYVGDESTAPVQPVEDESSPAF
                     LARALRHKLEAFHGTSDPAAVEAAWPHLNSSDRNIRYAARVAVEHQDPKLWAEKALAD
                     KRPTAVIQLMIALARAGDKSLQPRIVEKLNSLPYERMTEDQLTAAARAYGLCFIRMGQ
                     PSEALAKSVIDRVKGLFPNDSEPVSREFCTLLVYLRWPGVIAPAMEKLATGQTQQDQM
                     YYEFVLRNVAELMSHEQLVAYFSWMNLAQSSYKGGNSFVKFVQQIRNDATARLDEKQK
                     QALKDVIEGKQSVEVVKLETTRQFIHNWQMEDLLPLVDQVESGRSFEKGRAAYEAAQC
                     YKCHRFAGEGGSTGPDITGVGSRFNTVYLLESLIVPSKAISDQYLGSVIRTLDGEIIT
                     GRVIEESEKELKVRTDPFARELLTIAKDNIDAHQQSRVSEMPQGLINTLTKEEILDLV
                     AYMRSAGNAQDKAFAPAGK"
     gene            complement(9119..9631)
                     /locus_tag="VFV87_22255"
     CDS             complement(9119..9631)
                     /locus_tag="VFV87_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5106566.1"
                     /translation="MIDPTNTRHHSELVDPTAYIAPGAVVLGNVTLGSESSVWFGAVI
                     RGDTDAIKIGRQTNIQDGCVLHADEGVPCTIGERVTVGHAAIIHGATIEDDCLIGMRA
                     VVMNGAKIGKGSVVAVGSVVLEGMEVPPGSIVMGQPARIKREVTEQDAARIRHAAEHY
                     VAAAKVYRES"
     gene            9762..11132
                     /locus_tag="VFV87_22260"
     CDS             9762..11132
                     /locus_tag="VFV87_22260"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5106567.1"
                     /translation="MTELPTIAKAAKQMQEGELRPVELVESCLTRIDQLDERLQAWVH
                     VDANGAYREAERLGKLLDDGESLGPLHGVPVGVKDLIDVAGWPTKCGSVLREGHVAQR
                     DAAVVTSLRKAGAIILGKTVTTEWASFDPAPTRNPWNLEHTPGGSSSGSAAAVAAEMC
                     LAALGTQTGGSIIRPAAFCGVCGLKPGFGEVAMDGIAPLTLYLDHVGPIARRVSDLYL
                     IWQALIAGTHGSLESHYSKRWADTDLETWIGAYGLNKTLFVLDGPLLERMQPAMREVY
                     QCALARLQSSLHTRELPLPESFRDIHRWHRTIMAAEAAAYHRELFPRERARYGREIGK
                     LLDEGLALSAVDHAEALIRREEMRGELARLLHQHEDLLGCLVMPATLGPAPGMETTGD
                     PAFNSPWSYVGWPSLTIPCGLAADGLPCGLQFIAATPPQVFAMAGLCERILQFHERPA
                     ILEDAS"
     gene            11129..>12739
                     /locus_tag="VFV87_22265"
     CDS             11129..>12739
                     /locus_tag="VFV87_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5106568.1"
                     /translation="MRYLLTLAFVLTCHGAANAADADPLKPPAIETQDVPVIPPEFAD
                     RLSQYQSTRAAGFAGWSPDGNGMLVRTRFGNSVQLHRVYQPGGRREQITFEGEPVDGQ
                     FLPEVKDGAILLTMSAGGSENDQIYFYDRANFKTHRLTDGKSKNRIDAIRDDGQQLVI
                     ASNQRNGRDTDLYLADPRQPGSMKMLMEVSGEFWGAADWSPDGEELLLLQYVSANESY
                     PAWFNIKTGKRTPLRLPPGEKVAFSSLAYSADGKSVLWASDAGGEFRRLGRLDRSNYD
                     FEWITDDIPWDVSGLDVDRTSGRVAFTVNADGASQLYLLTPTDGGKFERREVKLPTGI
                     ASSLEFSPDGKQLGFTLSLPNAPADAYSLDLESGDLIPWTFSEVGGLNPATFISAERI
                     RFPSFDGREIPAWYYKPRTATAENKAPVLIQIHGGPEGQAQPYFSGTTQFFLNELGVA
                     VILPNVRGSAGYGKTYLKLDNAELREDSVKDIGALLDWIAKQPELDASRVAVSGGSYG
                     GYMVLASLVMHGERIKAGIDNVGIANFIT"
BASE COUNT         2241 a         3994 c         4198 g         2306 t
ORIGIN      
        1 aaacatcatt tgcaggaagt tggccgtgta cgacaggtcg ttctgcggat agacgaacgg
       61 ctggccgacc gattttttgt agctgtaggc ggcgatggtg gggagcttgg cgagcaggcg
      121 gtggatcgag acttcgacct gccgcggatc gcccggatcg agcgaatcct ggtagaaggt
      181 ggagagggcg ctggtggcgg cggagaggat ggccattggg tgggcatcgc gcgggaagcc
      241 gccatagaac ttgcgcatct cctcgtggag catcgtgtgg cgaaggatgg tgccgcggaa
      301 ggtttgcagc tcctgttcgc tcgggagctc gccatagatc aggaggtgag cgacttcgag
      361 gaagtcgcag ttggcggcga ggtcttcgat cgcgtagccg cggtagcgga gaatgccgcg
      421 ctcgccgtcg aggaaggtga tgtggctgct ggtggaaccg gtattggcgt agccttcgtc
      481 gagcgtggta cagccggtcg tggcgaggag cttggagata tcgatcccgg gttcgtcctc
      541 ggagccgacg acgacgggca gctccaggtc cttgccgcgg acatggagtc gggcgacctc
      601 ggtggattgg ggcgatctct cctgggcgta gctcatgagg aaagtagctg gaggagggga
      661 agcgcgagca gccgcgggcg gagcgacatc agcgctgagt atagcggtgc cgcggcgggt
      721 ttagaagcgg cgcgggagct ggcgcctgaa gggcgcgaat gcgggccgcg tggcctatcg
      781 cggaacgggc gcgcccggct gcggaggcgc ggttgcagtg gaactgtcgt agatgctcca
      841 gacctgcgtg ccgtggctga gcttctcgac aatttttttg tagtccccgt cgcgcttgcg
      901 aagttcgatc gctttcttaa tctccttctg ggcgtcgagg aaggagactt gccccgcggg
      961 ggtgcgctcg atgacgcgaa cgatgtgata gccctgatcg tcctcgatga tctggctcaa
     1021 cttaccgact tccagcgtga acagtgcctg gttgatcggc tcggaggcga ggctgccttg
     1081 cgaagtccaa tcgtaatagc ccccctgctt cgcgttgggt tcctgcgagg acttctgggc
     1141 gacggccttg aacggggcgc cgaagaagac ctcattcccc attgcggcaa tggcgctata
     1201 tgctgcgccg cgattcggga aattggcaga tttcgcggtc aggatctcaa agcgagcctt
     1261 ggccggcacc gcgtagtcgg cggcgtgctc gtagtagtaa tcgagcatct cctggtgggt
     1321 cacttccggg ttgaagttga tgtgcgtggc gatcccggtt cggccgagct tgtattcgcc
     1381 gtaatagcgg acctgctttt cgagcgtgga gccgaagcgg cggagaagaa gatcgagctc
     1441 gccgagagtt tccacctggt tgtcgcgcat caagagggcc agacgcgtga cgttcggatc
     1501 gcggcgcatc agctcctgga tctgatcggg cttggcgtcg gcgaccttct tccgcatttc
     1561 gcggaggtcg ttctcgaagc tctcgctgac cttcttggtg acgttggcct ggacctcggc
     1621 cagcttgtcc ttgggggctt tcttgagcag gtcgcgctcg aactccagat acatcagctt
     1681 ggtctcgatc atctggttga gcgtctggcg cgtgagcggc tcgcggtact tttcgatttc
     1741 gtgttgctcc aaggggttgg tcgccttgtc cagcatgggc tggagcaact gattcacgat
     1801 gggcgagaca tcgccgtaga ggatgaactt gtcgccgacc gtggcaatga tcctgcccgg
     1861 ttcgaaaagc tcacccttgg ggattggcag ttggggttcg gtgttcggtg ttcggggttc
     1921 ggcagtgacg aggggcgagg gagggccggc tggttctggg agtggatagc ggaggctttc
     1981 cgtcggcgcc gcggtcgcgg gttgcatcgg gccgggcatc ggagcttgtg ccgcccccgg
     2041 gacgggattc tggccgaagc gaaatgtcgg ctgctggacg gtgatcgggt agcgattcgg
     2101 gtcgggctgc tggccgctgg cggcgcgcac cccgagcgcc agaatcagca gcgttgccag
     2161 gcgcgcagca cgtggtgtca ctcgagtcgc ctcactaccg taggcttctc gctccgcgag
     2221 aagcaaggtg ctttccaggc gcgcaaaccc agcgcgccga atgagtccat tgcgactcgc
     2281 ggagcgagtc gcctacgatg gggccgggat agtagtggcc tatggcggct gccgcaacag
     2341 cgattttgtg attcgccaga tccggtcggt ctctgtaagt tcttttggca gcgggagata
     2401 ggcgctcttg tcgtcgacga tccgcagctt gcccttgtgc tggcgggcga gctgctcgat
     2461 tcgcgggcgg ctgtcgtagc ggaagacgac gaagtcgttc tcgatgaaga ccgtcgagat
     2521 ttgccagctt gccgcgtcca ttttcagttc tgccagcagc agcagccgct cgaccggctg
     2581 cggcggctcg ccgaagcgat cgaccagctc cgagcggaaa tcggtcaggt cgtcgtactt
     2641 ggcgacgcgg gccagtcggc gatacaggtc aattttcagc cgcaagtcgg gcacataatc
     2701 cgccggcagg aaggcatcgc ccggcagatt gacatcgacc tccagccgca ctttcggcgg
     2761 catttgctgc aaggcccgca cggcgttttc cagcagcgtg caatacagct cgtagccgac
     2821 cgccgcgatg tggccgcttt gctgggtgcc cagcagattc ccggccccgc gaatttccag
     2881 atcgcgcatc gcaatcgcga agccggcgcc catctcgctg aactcttcaa tcgcccgcag
     2941 ccggcgggcg gcgtttggcg tcagatgttt gtgcggctcg atgagcagat agcagtaggc
     3001 gcgattttta taacggccga cgcgtccgcg aagctggtgc aaatcggcca ggccatagcg
     3061 gtcggcttcg tcgatgaaga tcgtgttggc gttgggaatg tcgaggccgc tttcgacgat
     3121 cgtcgtgctg agcagcagat cgaaccggcc ggcgacgaaa tcgaccatca cctcttccag
     3181 ctcatgatcg ggcatctggg cgtggccgat ccgcagcttc gcctcgggcg cgatctcgcg
     3241 caggcggttg gcgatcagct cgatgtcgtt cacgcggttg tggacgaagt agatttgccc
     3301 gccgcgggcc agctcgcgga gcaccgcgtg gcgaatcagc ccgttgtccc agcgggccac
     3361 ctttgtttcg acggccagcc gatcctccgg cggcgtttcc aggttcgaga tgtcccgcag
     3421 gccgacgagc gacatgtgca gcgtccgggg aatcggcgtg gccgaaagcg tgagcacatc
     3481 aaccgtggct cgcagcatct tcagccgctc tttcacatcg acaccgaacc gctgctcttc
     3541 gtcgatgatc accaggccca ggttcttgaa ctcgatgtcc ttcgaggcca cgcggtgcgt
     3601 gccgatcaca atatcgatct ttccgctctt caggcccgcg accgcggctc gctcctcgtc
     3661 ggcggtgcag aagcggctga gcttggcgat atcgaacggg aattcggcca tccgctcgct
     3721 gaagctgcgg aaatgctgct cggcgaggat cgtcgtcggc acgaggatcg ccacctggta
     3781 accgtgatcc acggccttga acgcagcccg catcgcgacc tcggtttttc caaatccgac
     3841 gtcgccgcag agcaggcgat ccatcggccg cgggcgctgc atgtcggctt tgatggccga
     3901 gatcgcgccc agttggtcgg ccgtttcgtg atacgggaac gagtggtcga actcgcgctg
     3961 ccagtcgctt tcgctgccga aggagatacc cggccgcgag cggcggatcg cctgcatttc
     4021 gagcatttcg accgccagat cgtgaactgc ggcctccgcg tccttttttt gccgcagcca
     4081 cgtcttgccg ccgatcttag ccagcgaggg ccgcgttttc gtgccgccga cgtatttctg
     4141 caccaggtcg atctttactg ccggcacgta gattttcgtg ccgccgtcga attcaatctc
     4201 caggtgttct tcgtgctgcc cttgccgctc gataagtttc aggccgcggt accgtccgat
     4261 gccgtgggcc aggtgcacga cgaggtcgcc ctcgcgcagg tcgaggaacg aatcgatgac
     4321 cttgcccagc ctccgccgcg gcagccgccg caactcgccg cgatgaaaca gctcgccggc
     4381 ggtgaggatc agcattcctc tcccctcggg cgctgtaggg aacgccctcc gtggcgttcc
     4441 ggtaactgcc agttcgttcg ccgcgcccga gcccggaacg ccacggaggg cgttccctac
     4501 aggcgggcgc aatcgaaaac cagcccgcag cgaaccaatt ggaaagtgca gtcgtccggc
     4561 cgccgcgagc ctggtcgtcg ccagaatctc ccgcaggcgc tcgacttccg cttcggtcgg
     4621 gcagacgagg aagacttcgt cctcatgggc gatcgaatcg agttcgtcgc gcacgcggct
     4681 gatgtcgccg ctgaaccgct cgaccgattc gcagggcaaa tggcagtgga cgccagccgt
     4741 gccggactga atctcgccag ccgtggcgac tgcgaagcgg ccacattcgg cgagcacggc
     4801 attcaccgaa tgaaactccg ccggcttttc gacgcgctgg aggtagtgct tgccttcttc
     4861 gccaattcgc tcgggatcga cgaggagaat ctggcttccg ggcggcaagt aatcggtgag
     4921 atggccggcg atcgttccct ctccctctgg gagagggtta gggagagggc gtttgagtgc
     4981 gaggagagtc cccgcaccct caccccggcc ctctcccaga gggagaggga ggtgcgtgtc
     5041 cgctcgcagg acggtcacgt caatctcgtc cagcgtttcg acgctccgct gcgtgccgac
     5101 ttcgaacgtg cggagcgact cgatctcatc gtcgaacagc tcgacgcgga ccggccgcgg
     5161 ccagtcgggg gcgaagatgt cgatgattcc gccgcgatgg gagaactcgc ccggcagctc
     5221 cacggctgtg gtgtgatgaa agccatgctc cacgagccat cgcaggaatg tctcgacgtc
     5281 gatccgctcc tccttgcgaa gctgccgcgt agccgcggcg atcgcctcct tcggcgtcga
     5341 tggttgcagg agggcctgaa tcgaggtgac gaggatcggc gtgagtgctg tagggaacgc
     5401 cctttgtggc gttccgtgat ccgcgcgagc gccctcttcc ggatccggct cgttgcgccg
     5461 cgacttgatg aagttgccca ggccacgcgg cgtcggcacc ggcgatgcgt cggcacccgc
     5521 caattttggc ccagccggat tcagcagccg cttcagcacc cgcagccgct cgccgaaggt
     5581 ctcgtcatgc acgagccgct cgtcgggcgt ggtttcccag gtcggataga gctgcggctc
     5641 aaccgcggtg aagagctcca aatccgcggc cagatcatcg gcgtcgcggg ccgtcgggca
     5701 aatgacgagg agcggtggcg cgaccgtcga tccgccaccc gagtcgatct cctctcgcgg
     5761 agcgagaggc ctacggtcgg ccagccccgc cgcaatcagc gcgcaggccg acccccagac
     5821 tccgtcgaac gacgcggggc ggccggcggc caactccgcg agcgcttcgc taaagcctgc
     5881 gtaggattcc agttgtgggg caagctcccg catccgctcg gaagcggtag cgtgcggcaa
     5941 aactgccgtc atggcgaggg aattctagcg gccagccggc cgtgggcgat aggcgaatcg
     6001 cccggccggg caggtagcgg actgcacctg ccggcaacta tattgggcat gaacgatcct
     6061 cgccggccgc gcgtaaatgt ctgcatggcc cgcttcccgc ccgccaagtc tctgccctcg
     6121 ttggagttac gccgaatgcg ttcgttcctt ctcctcgcgc ccgtcgccgc aatactgctc
     6181 cttatgttcg cccattccgc gacagccgct gacccggttc cccagtggct gtggacgaac
     6241 aaagagccca aggagaatga gtcagccttt ttccgcaagg cgatcgagct ggacgctgct
     6301 cccaaatcgg ctacgttcta cggcgcctgc gataatgtaa tgacgctctt cgtcaatgga
     6361 gagcaggtca cgcagcattc ggcttgggat gcggccacgc gcgagaactt gacgaagcgg
     6421 ctcaaggcag gcaaaaacgt catcgccatc cgggccagca atcagggggg gccggccgcc
     6481 ttcatcgccc agatcaacat cgagcgggcc gacggcacca agctggtgat cgtcaccgac
     6541 gaaagctgga aagccgcatc cgaggcccgg ggcaacttcc ggacggagcg ctatgacgac
     6601 agcgcgtggg gcaagcccca tagcttcggc aagctgggca tcgctccctg gggcgatatc
     6661 gccctgactt ccgccaccgc cagcggctcg gcggcgacgc ccgtcgaagc catccagacg
     6721 ctgccgggat tcaaggtcga actgctctac tccgtgccca aggaatcgga aggctcgtgg
     6781 gtcaatatga cctccgacaa caagggccgg ctgatcgtct ccgaccagta cggggcgctc
     6841 tatcgcgtga tgccgggcca ggacgcggaa accacgcagg tcgagcaact cgcggtgccg
     6901 ctaggcgagg cgcaggggct gctctatgcg ttcgatgcgc tgtacgtcgt ggtcaaccgt
     6961 ggccagagct acgaaagcgg cctctatcgc ctgaccgaca ccaacggcga cgaccagttc
     7021 gacaaggtcg agcagcttaa gaaaatcaat ggtggcggcg agcacggccc gcacgccgtg
     7081 aagctcggcc ccgatggcaa gttgtacgtc atcgccggca atcacacgca aactcccgac
     7141 ggactggatc ctgcctcacc ccaccgcaac tgggccgagg acctgctgtt gccgcgaaac
     7201 cccgatggca acggccacgc gacgggcgtg atggcccctg gcggctgggt ctgccggacc
     7261 gataaggacg gccgctactg ggagattctc tgcgccggct tccgcaacga gttcgacatc
     7321 gacttcaaca ccgacggcga gctcttcacc tacgacgccg acatggaatg ggataccgga
     7381 gccccgtggt atcgccccac gcgcgtcaac cactgcgtga gcgccggcga atacggctgg
     7441 cgatacggaa cgggcaagtg gccggactat tccgccgaca gcctgggcgc cgtcgtcgat
     7501 atcggcctcg gctcgcccac gggcatcgtc ttcggcaccg gagcgaaggt cccggcgaaa
     7561 taccagcagg cgttgtacat caacgactgg acgtacggca agatctacgc ggtccacatg
     7621 acgcccagcg gcgcaagcta caccggtacg ttcgaggcgt tcatcaccgg ccgcccgctg
     7681 ccggtgacgg acgtcgtcgt gcataccgat gggcagctct atttcacgat tggcggccgg
     7741 cggacgcagt cggggctgta ccgcgtaacg tacgtgggag atgaatcgac cgcacccgtc
     7801 cagcctgtgg aggatgaatc gtcgcctgca ttcctggccc gcgctctgcg gcacaagctg
     7861 gaagcgtttc atggcacgtc cgatccggcc gccgttgaag ccgcctggcc gcatctgaac
     7921 agcagcgacc gcaacatccg ctacgctgcc cgcgtcgcgg tggaacacca ggacccgaag
     7981 ctgtgggccg agaaggcact ggcagacaag cggccaacgg cggtgattca gttgatgatc
     8041 gccctggccc gcgccggtga taaatcgctc cagccgcgga tcgtcgagaa gctgaattcg
     8101 cttccttacg agcggatgac cgaggaccag ctcaccgcgg cggcgcgggc gtatgggttg
     8161 tgcttcatcc gcatgggcca gccaagcgag gccctggcga agtcggtgat cgaccgcgtc
     8221 aagggccttt tcccgaacga cagcgagcct gtcagccgtg agttctgcac gctgctggtt
     8281 tatctgcgct ggccgggcgt gatcgcgccg gcgatggaga agctcgccac aggccagacg
     8341 cagcaggacc agatgtatta cgagttcgtg ctgcggaacg tcgccgagct gatgtcgcac
     8401 gagcagcttg tcgcttattt cagttggatg aacctggccc agtcgtccta caagggcggc
     8461 aacagctttg tgaagttcgt tcagcagatc cgcaacgacg cgaccgcccg gctggacgaa
     8521 aagcagaagc aggctctcaa ggacgtgatc gaaggcaagc agagcgtgga ggtcgtcaag
     8581 ctcgaaacga cccgccagtt catccacaac tggcagatgg aggatttgct gccgctggtc
     8641 gatcaggtcg aaagcggccg ttcgtttgag aaagggcggg cggcgtacga agcggcccag
     8701 tgctacaagt gccaccgctt cgccggcgaa ggaggcagca ccgggccgga catcaccggc
     8761 gtcggcagcc gcttcaacac ggtttatctg ctcgaatcgc tgatcgtgcc ttccaaggcc
     8821 atttcggacc agtatctcgg ctcggtcatt cgtacgctcg acggcgagat catcaccggc
     8881 cgcgtgatcg aggagagcga gaaagagctg aaagtccgca ccgacccctt cgcccgcgaa
     8941 ctgttgacaa tcgccaagga caacatcgac gcccaccagc aatcgcgcgt cagcgagatg
     9001 ccgcaggggc ttatcaatac gctcacgaag gaagaaattc tggatcttgt ggcttatatg
     9061 cgctctgcgg gcaacgccca ggacaaggca ttcgcgccgg ccgggaaatg acgacaggtc
     9121 aggactcgcg atagactttc gccgccgcaa cgtagtgctc ggcggcgtgg cgaatccgtg
     9181 cggcgtcctg ctcggtcact tcccgcttga tccgcgccgg ctggcccatg acgatgctgc
     9241 cgggcgggac ttccatgcct tccaggacga cgctgccgac ggccacgacg ctccccttgc
     9301 cgatcttcgc gccgttcatc acgaccgccc gcatgccgat caggcagtcg tcctcaatgg
     9361 tggccccgtg gataatcgcc gcatggccca ccgtcacacg ctcgccgatc gtgcagggca
     9421 cgccctcatc cgcatgcagc acgcagccat cctggatgtt cgtctggcgg ccgattttga
     9481 ttgcgtcggt atcgccgcga atcaccgcgc cgaaccagac gctcgactcg gatccgagcg
     9541 tcacgttgcc gaggacgact gccccggggg cgatgtaggc cgtgggatca accagctcgg
     9601 agtggtggcg ggtgttggtg ggatcgatca cgagactgtt cctagccggg accaaacctg
     9661 gtcccggaga actgcgagtg gagcatctac cggccgcgct ccgggagcaa gtttgctccc
     9721 ggctattgcg cggggctgcg ttgttgagta atgttacctg gatgaccgaa ctgccgacaa
     9781 tcgccaaggc ggcgaagcag atgcaagagg gcgagctgcg cccggtggag ctcgtcgaat
     9841 cgtgcctgac ccgcatcgat cagctcgacg agcggctgca ggcctgggtc catgtcgatg
     9901 ccaacggtgc gtatcgcgaa gccgaacggc tcggaaagct gctggacgac ggggaatcgc
     9961 tcggcccgct acatggggtt ccggtcgggg tcaaggatct gatcgacgtg gccggctggc
    10021 cgacgaagtg cggctccgta ctgcgcgagg gacatgtcgc ccagcgcgac gccgcggtgg
    10081 tcacgagtct gcggaaggcg ggcgcgatca tcctcggcaa gaccgtcacg accgaatggg
    10141 ccagcttcga tcccgcgccg acgcgcaatc cgtggaacct ggagcacacg cccggcggct
    10201 cgtcgagcgg cagtgcggca gcggtcgcag ccgagatgtg cctggcggcg ctcggcacgc
    10261 agaccggcgg ctcgatcatc cgcccggcgg cgttctgcgg cgtctgcggc ctgaagcccg
    10321 gattcggcga agtcgcgatg gatggcatcg ccccgctgac cttgtacctg gatcacgttg
    10381 gcccgatcgc ccgccgcgtc agcgatctgt atctcatatg gcaggcgctc atcgccggca
    10441 cgcacggcag cctggagagc cactattcca agcgctgggc cgataccgac ctggaaacct
    10501 ggatcggagc ctacggcctg aacaagacgc tcttcgtact ggacgggccg ctcttggagc
    10561 gcatgcagcc ggcgatgcgc gaggtctatc agtgcgcgct ggcccggctg cagagctcac
    10621 ttcacacgcg cgagttgccg ctgcccgaat cgttccgcga tattcaccgc tggcaccgca
    10681 cgatcatggc cgccgaagcc gccgcctatc accgcgagct gttcccgcgc gagcgagcgc
    10741 gctacgggcg cgagatcggc aagctgctcg acgaagggct cgccttgtct gccgtcgatc
    10801 atgccgaggc gctgatccgc cgcgaggaga tgcggggcga gctggcgcgg ctcctgcatc
    10861 agcacgaaga cctgctcggc tgcctcgtga tgccggcgac gctcggtccc gcgccgggaa
    10921 tggaaaccac gggtgacccg gcgttcaatt ccccctggag ctacgtcggc tggccgtcgc
    10981 tgacaattcc ctgcggcctc gccgccgacg gcctgccgtg cggcctgcaa ttcatcgccg
    11041 cgacgccgcc gcaggtcttc gcgatggccg gcctgtgcga gcggattctg cagtttcacg
    11101 agcgacccgc gatcttggag gatgcatcat gaggtatttg ctgactctgg cattcgtgct
    11161 cacctgccac ggtgcggcaa acgcggccga tgccgatccg ctcaagccgc cggcgatcga
    11221 aacgcaggac gtgccggtga ttccgccgga gtttgccgat cggctgtcgc agtatcaaag
    11281 cacgcgggcc gcgggctttg ccggctggtc gcccgatggc aacggcatgc tggtccgcac
    11341 gcggtttggc aactcggtgc agctccaccg cgtctatcag ccgggcggcc ggcgcgagca
    11401 gatcacgttc gaaggcgagc cggtcgatgg ccagttcctt cccgaggtga aggacggggc
    11461 gatcctgctg acgatgagcg ccggcggcag cgagaacgac cagatctact tctacgaccg
    11521 ggcgaacttc aagacgcatc ggctgactga cggcaagagc aagaaccgga tcgacgcgat
    11581 tcgcgatgac ggccagcaat tggtcatcgc cagcaaccag cgcaatggcc gcgatacgga
    11641 cctgtatctc gccgatccgc gccagcccgg ttcgatgaag atgctgatgg aagtcagtgg
    11701 cgaattctgg ggcgctgccg actggtcgcc ggacggcgaa gagctcttgc tgttgcaata
    11761 cgtgtctgca aacgaatcgt atcctgcatg gttcaacatc aaaaccggaa aacgcacccc
    11821 actacgactg ccgcccggtg aaaaggtagc attcagctcg ctcgcatact ctgccgacgg
    11881 taagtctgtc ctttgggcgt ccgatgcggg cggtgagttt cgccgactgg gtcgtctcga
    11941 ccggtcgaac tatgacttcg agtggatcac ggacgacatc ccgtgggatg tgagcggtct
    12001 ggacgtcgat cggacgagcg gccgggtcgc cttcaccgtc aacgccgacg gcgcgagcca
    12061 gctctatctg ctcacgccga cggacggagg caagttcgag cggcgcgaag ttaaactgcc
    12121 gaccggcatc gcctcgtcac tggaattctc gcccgacggc aagcagctcg gtttcacgct
    12181 ctcgctgccg aatgctcccg ccgacgccta ttcgctcgat ctcgaaagcg gcgacctgat
    12241 tccgtggacg ttcagcgaag tcggcggcct caacccggcg acgttcatct cggccgagcg
    12301 gatccgcttc ccctcgttcg acggccgcga gattccggcc tggtattaca agccgcgaac
    12361 tgcaaccgcc gagaacaagg ccccggtgct gatccagatt catggcggcc cggaaggcca
    12421 ggcccagccg tacttctccg gcacgacgca gttctttctg aacgagctgg gcgtcgcggt
    12481 catcctgccc aacgtccgcg ggtctgcggg atacggcaag acgtatctca agctcgacaa
    12541 cgccgagctc cgcgaggaca gcgtcaagga catcggggcc ctgctcgact ggatcgcaaa
    12601 gcagccggag ctcgatgcct cgcgggtcgc ggtcagcggc ggctcgtacg gcggctacat
    12661 ggtgctggcc tcgctggtga tgcacggcga gcggatcaaa gccggcatcg acaacgtcgg
    12721 catcgccaat ttcatcacg
//
LOCUS       DASUGB010001174         5306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1104479, whole genome shotgun sequence.
ACCESSION   DASUGB010001174 DASUGB010000000
VERSION     DASUGB010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104479"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFV87_22270"
     CDS             <1..517
                     /locus_tag="VFV87_22270"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106569.1"
                     /translation="TRDRYGRNAFGQRLVMARRLVEAGVPYVAVRTADWDDHAQLVSR
                     MKARCPEFDRGIAALITDLRERGMTQDVLVVAMGEFGRTPRVNQNGGRDHWPAVASVL
                     ISGGRYRMGQVIGATDSHGAYVAAAPYQPQSVLALVYRHLGIDPSLTFLDYTGRPRYV
                     LEERQSITELI"
     gene            complement(501..1997)
                     /gene="cobA"
                     /locus_tag="VFV87_22275"
     CDS             complement(501..1997)
                     /gene="cobA"
                     /locus_tag="VFV87_22275"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5106570.1"
                     /translation="MATGKVYLVGAGPGDPGLITLRGVELLRRADAVLYDYLVNPAIL
                     EHCRPGAVRVSLGKHGSGRVLSQRQINERLVAMAQTFQTVVRLKGGDPMVFAHAAEEL
                     DCLVQSGIAFEIVPGVTAALAAASYAGIPATHRDAASAVALVTGQENADKAGSALDYA
                     ALARFPGTLVFYMGVTTAEHWSQALIAGGKPATTPVAILRHVSLPDQLRIDTTLGEVA
                     RVVREHKLRPPVVFLIGEVAAHGAAWSWFEKRPLFGQKVLVTRPAHQAADLAQPLAEL
                     GAEVLLQPAIEIRPPADWSPVDRALELLDRFDWLVVSSSNGVRFLLERLPAIGRDLRL
                     LSKLKIAAIGPGTAEELARFHLWADLVPDEYRAESLAEALKGGAAGKRFLLVRASRGR
                     EVLADELTKAGGIVEQVVVYESVDALSPQAEIAELMAAGRIDWTTVTSSAIARSLVRM
                     FGDTLRRTKLVSISPITSATLRELRHEPAAEATEYTMPDIVAAIRSAQ"
     gene            2099..3406
                     /locus_tag="VFV87_22280"
     CDS             2099..3406
                     /locus_tag="VFV87_22280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106571.1"
                     /translation="MPADPPASLLITPDLRRRLQQRYEEALRLKNHQPPQHARIHELL
                     AECLRADPGNTLYLEALLANVRKWQPRKGSGIPGWLTGWISSRSGESPAGGERTEYSV
                     LSTQYLADHPIDETVAREALRNSPDLILSGCDDANTFVSLAAACAAFDLDQAAVRHLR
                     LASESAPDDAAVARMLARALTRQGHFDEAAAHWQRLVAIAPDAEAQQALDDLQVPSDL
                     ESSDRQLAEASAAGGEDLALRRERENLRLAHIEQQVATARQRARHDPHPKAQALAAAL
                     AAELLRQEIEILHLRSERLPGDRQLRLDLARKLKQVGNYSGAIQRLEEVRGDPALAAE
                     VLLELGECWQHLRQFERALDFYRQAVAAAETIDEQREPRPLVAALISQQEPRPLVAAL
                     YRTGVLAAAMGKPDEARGAFARVIALDPAYKDARERLDNLPPN"
     gene            3486..3758
                     /gene="rpsT"
                     /locus_tag="VFV87_22285"
     CDS             3486..3758
                     /gene="rpsT"
                     /locus_tag="VFV87_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5106572.1"
                     /translation="MHTKSAKKRLRQSLEKRAKNRSIKSSIKTQIRKVRAAVAAGDIA
                     TAEAEARLAATKLDRAGVKRVIHPNAAARKKSRLAHLIVSAKAKKA"
     gene            3798..5024
                     /locus_tag="VFV87_22290"
     CDS             3798..5024
                     /locus_tag="VFV87_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106573.1"
                     /translation="MFRRHVILFSGWAALSIFAGLATAADDSLALFQRRILPILSAKN
                     ASSCSECHLSGVDLKDYIRPTQAETFAALKSAGLIDASKPEASKLLAFIARKPDKPSL
                     ITDKVRQEEYEAFRAWIVAAVKDPELAKTKAIDGSIGPGLPDEVIQHARKDRVLASFV
                     DNVWSEVGRCAACHSPDRNQQQVKKHGEHISWIKLGNPQATLDHLREHELIDVDAPEK
                     SLILLKPLNQVMHGGGQKMAFGDRTYKQFRRFLDDYSATLAGKYTKADQLPAENAEVS
                     AVTEIWFKLEGIPAEYDQLVLQVDLYRWDDKSQAWTPDRWATADRPVFGKGGLWQNHL
                     SLTAPRDSARAAEFARRPSLPAGKYLAKVYLDRQHKFEKQFPAELGPDDLLGQVEVNS
                     RWPAGYGSMTVAKLPK"
     gene            complement(5045..>5306)
                     /locus_tag="VFV87_22295"
     CDS             complement(5045..>5306)
                     /locus_tag="VFV87_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5106574.1"
                     /translation="GPIHHAEATGSGGNHFVSERYPDFVTIAKGYHWGAATISKKADL
                     VPALEEMISSDGPYLLNVQVPYVEHVLPMIPGGKTVDDIILQ"
BASE COUNT          967 a         1803 c         1650 g          886 t
ORIGIN      
        1 aacccgcgat cgctacggcc gcaacgcctt cggccagagg cttgtgatgg cccgccggct
       61 ggtcgaggcg ggcgttccgt acgttgccgt ccgcacggct gattgggatg accatgccca
      121 gctcgtctcg cggatgaagg cccgctgccc ggagttcgac cgcggcattg cggccctgat
      181 caccgacctg cgcgagcggg ggatgacgca ggatgtgctg gtcgtggcaa tgggtgaatt
      241 cggtcgcacg ccgcgggtca atcaaaatgg tggacgcgac cactggcccg ccgtcgccag
      301 cgtgctcatc tccggcggcc gatatcggat gggccaggtc atcggcgcga ccgacagcca
      361 cggcgcctac gtcgccgccg ccccctatca accgcaaagt gtgttggccc tggtgtatcg
      421 ccatctcggc atcgacccat cgctcacgtt cctcgactac accggtcgtc cgcgctacgt
      481 cttggaagaa cggcaatcga ttactgagct gatctgattg cagcgacaat gtcgggcatc
      541 gtgtactcgg ttgcctcggc ggcaggttca tggcgcagtt cacggagcgt ggcggaggtg
      601 attgggctga tgctgacaag tttggtccgg cggagtgtgt cgccaaacat tcgcaccagt
      661 gagcgggcga ttgccgagct ggtcaccgtc gtccaatcga tcctgccggc tgccatcagt
      721 tcggcaatct ccgcctgcgg cgaaagggca tcgacgcttt catagacgac gacttgctcg
      781 acgatcccgc cggctttggt cagctcgtcg gccagcacct cccgcccgcg gctggcgcga
      841 acgaggagga atcgcttgcc ggccgcgccg cctttcaggg cctcggctaa cgattcggcc
      901 cgatactcgt cggggaccag gtccgcccag agatggaacc tcgccaactc ctcggctgtt
      961 cccggcccga tcgcggcgat cttgagcttg ctgagcaacc gcagatcgcg tccaatcgcc
     1021 ggcaaccttt ccaggaggaa gcgaacaccg ttgctgctcg aaacgacgag ccagtcaaag
     1081 cggtcgagca gttccagcgc gcgatcgacg ggcgaccaat ccgccggcgg acgaatttca
     1141 atcgccggct gcagcagcac ttcggctccg agctccgcca gcggctgggc gaggtcggcg
     1201 gcctgatgcg cgggccgcgt gacgaggact ttctgcccaa atagcggccg cttctcaaac
     1261 cacgaccagg cggccccgtg cgccgcaact tcgccgatga ggaatacgac cggcggccgg
     1321 agcttgtgct cgcggaccac gcgggcgact tcgcccagcg tcgtgtcgat ccgcaattgg
     1381 tccggcaggc tgacgtgccg caggatcgcg acgggagtcg ttgctggttt gccgccggca
     1441 atgagggcct gcgaccaatg ctcggctgtc gtcacgccca tgtagaagac cagcgtgccc
     1501 ggaaatcggg ccaacgccgc gtagtcgagc gccgagccgg ctttgtcggc gttttcctgc
     1561 ccggtgacca gcgccacggc tgaagcggcg tcgcggtgcg ttgccggaat gcccgcatag
     1621 ctggccgccg cgagggctgc cgtcacgccc ggcacgattt cgaaggcgat gccgctttgc
     1681 acaaggcaat ccagctcctc cgcggcgtgg gcaaagacca tcggatcgcc ccccttgagg
     1741 cggacgacgg tctggaacgt ctgggccatc gccaccagcc gctcgttgat ctggcgctgc
     1801 gacaggacgc gtccgctgcc gtgcttgccg agcgaaaccc ggacggcgcc gggccggcaa
     1861 tgttcgagga tcgccgggtt cacgaggtag tcgtacagga cggcatcggc ccgccgcagc
     1921 agctcgactc cgcgcagcgt gatcagcccc ggatcgcccg gccccgcgcc gacgagatag
     1981 actttgcctg ttgccatttc gcgctgtagg gaccgcccct cagtggcggt ccgcccttgc
     2041 cgttggttcg cgtaaaactc ggaacgccac ggaaggcgtt ccctacaaga tacactctat
     2101 gcccgccgat ccgcctgcct ccctgctcat cacgcccgat cttcgccgcc gactccagca
     2161 gcgatacgag gaagccctgc ggctgaagaa ccaccagccg ccgcagcacg cccgcatcca
     2221 cgagctgctc gccgaatgcc tgcgggccga tccggggaat acgctctacc tggaggcact
     2281 gctcgccaac gttcgcaaat ggcagccgcg taaaggtagt ggcatacctg gctggttgac
     2341 cggctggatc tcgtcccgta gcggcgaatc gccggccggc ggtgaacgca ctgagtactc
     2401 agtactaagc acccagtacc tggccgatca cccaatcgac gaaaccgtgg cccgcgaggc
     2461 actgcgcaat tcacccgact tgatcctgag cggctgtgac gacgcgaaca cgttcgtttc
     2521 tttggctgcc gcttgtgcag cgttcgacct cgatcaggcg gcagttcgcc atctgcgact
     2581 ggccagcgag tcggcgcctg acgatgcggc ggtggcacgc atgctcgccc gcgcgctgac
     2641 gcggcagggg cactttgacg aggcggccgc gcactggcaa agactggtcg ccatcgcgcc
     2701 ggatgccgag gcccagcagg ccctcgatga cctgcaggtg ccgagcgatc tcgagtcgtc
     2761 cgaccgccag cttgccgagg ccagcgccgc cggcggcgaa gaccttgcac tccgccgcga
     2821 gcgcgagaac ctgcggctgg cacacatcga gcagcaggtc gcgacggccc gccagcgcgc
     2881 ccggcacgat ccgcatccca aagcccaggc cctcgctgcc gcgctggcag ccgagcttct
     2941 tcgccaggaa atcgagattc tgcacctccg ctcggagcgc ttgccggggg atcggcagct
     3001 tcgcctggat ctggcgcgca agctgaagca agtcggcaac tacagcggag cgatccagcg
     3061 gctggaggag gtgcggggcg atccggcgct ggctgccgag gtgctgctgg aactcggcga
     3121 gtgctggcag catctgcggc agttcgagag ggcgctcgat ttctaccggc aggcagtcgc
     3181 agcagcggaa accattgatg agcagcggga accgcgacca ctggtcgcgg ctttgatttc
     3241 gcagcaagaa ccgcgaccac tggtcgcggc tttgtatcga acgggcgtcc tggccgcggc
     3301 gatgggaaag ccggatgagg cacgaggggc tttcgcccgc gtgatcgccc tcgatcccgc
     3361 ctacaaagac gcccgcgagc ggctggacaa cctgccgccg aattgacata ctgggcgatt
     3421 cgccgtcgcg aaagcaggtc cggaatatcg acacaacgca tagccattga acgagttacg
     3481 acgcgatgca taccaagagc gctaagaaac gccttcgcca aagcctggaa aagcgagcca
     3541 agaaccggtc gatcaagtcg tcgatcaaga cgcagattcg caaggtccgc gccgcggtcg
     3601 ccgctggcga tatcgccacc gccgaagccg aggcccgcct cgccgccacc aagctcgacc
     3661 gcgccggcgt caagcgcgtc atccacccca acgctgccgc tcgcaagaag tcccgcctcg
     3721 cccacctgat cgtttccgcc aaggcgaaga aggcctgatg cccttccgag cggcggcccc
     3781 tgtttcggag tcgcccgatg ttccgtcgcc atgtgattct cttcagtggt tgggcagccc
     3841 tgtcgatctt tgcgggcctc gcaaccgcag cggacgactc gctcgccctc ttccagcggc
     3901 gtatcctgcc gattcttagc gccaagaacg cctcgagttg cagcgagtgc catctgtcgg
     3961 gcgtcgatct gaaagactac atccggccca cccaagccga gaccttcgcg gccctcaaat
     4021 ccgccgggct gatcgacgcg agcaagcccg aggcctccaa gctcctggct ttcattgccc
     4081 gcaagccgga caagccgagc ctgattacgg acaaggtccg ccaggaggaa tacgaagcct
     4141 tccgggcatg gatcgtggcc gcggtcaaag accccgagct ggccaaaacc aaggcaatcg
     4201 acggctcgat cggccccggt ctgccggacg aagtcattca gcacgctcgc aaagatcgcg
     4261 tcttggcgtc gttcgttgac aacgtctggt ccgaggttgg gcgctgtgcc gcgtgtcatt
     4321 cgcccgaccg caaccagcag caggtgaaaa agcacggcga gcacatttcg tggatcaagc
     4381 tcggcaatcc gcaggccacg ctcgatcact tgcgcgaaca tgagctgatc gacgtcgatg
     4441 cgcccgaaaa aagcctgatc ttgctcaagc cgctgaatca ggtgatgcac ggcggcggcc
     4501 agaagatggc tttcggcgat cgcacctaca agcaattccg ccggttcctc gacgactact
     4561 ccgccacgct cgccggcaaa tacaccaagg ccgaccaatt gccggccgaa aacgccgaag
     4621 tctcagccgt cacggaaatc tggttcaagc tcgaaggcat cccggcggaa tacgatcagc
     4681 ttgtgctgca ggtcgatctc taccgctggg acgacaaatc gcaagcctgg acgccggacc
     4741 gctgggccac cgccgaccgt ccggtcttcg gtaaaggcgg cctgtggcaa aaccacttga
     4801 gcctcaccgc cccgcgcgat tccgctcgtg cagccgaatt cgcccgccgg ccttctctgc
     4861 ctgccggcaa atatctggcg aaagtctatc tcgatcggca gcacaagttc gaaaagcagt
     4921 tcccggcgga actcggcccg gacgatctcc tcggccaggt cgaggtcaac tcgcgctggc
     4981 cagccggcta tggcagcatg accgtggcga agcttcccaa gtagaatctc gccgcggcgg
     5041 ttgctcactg cagaataata tcatccaccg tcttcccgcc cgggatcatc ggcagcacgt
     5101 gctcgacgta cggcacctgc acgttcaaga ggtacggccc gtcgctgctg atcatctctt
     5161 ccagcgccgg tacaaggtcc gccttcttgc tgatcgtggc cgcgccccag tgatagccct
     5221 tggcgatcgt cacgaaatcg ggataccgct cgctcacaaa atgattgcca ccactgccgg
     5281 tggcttcggc gtggtgaatc ggcccc
//
LOCUS       DASUGB010001175         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1937163, whole genome shotgun sequence.
ACCESSION   DASUGB010001175 DASUGB010000000
VERSION     DASUGB010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937163"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFV87_22300"
     CDS             <1..323
                     /locus_tag="VFV87_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106575.1"
                     /translation="WPQAAERLQRLALALPSDRRFLRRAGLATLQVGRHADSLEHWRR
                     LLAGLSSGSDDWLEAKYYQLVCLGATDRATAEKVWKQFKLLFPEVKSEVWKEKFAELE
                     TEFS"
     gene            424..>3148
                     /locus_tag="VFV87_22305"
     CDS             424..>3148
                     /locus_tag="VFV87_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106576.1"
                     /translation="MPPKPTAAPLDPDLLAAFRQQGAAIVAQERGLTAVCRVKLARLA
                     GSLGIPDEQIDAAIRALHESGPKAPPNPLAEKFRDRLRKDLAGKTRAIIGPTIEAEII
                     AAAARKYSLDEPTSRQVLGEVAEEIGVTRITATDAIDSLATQIEQTVGESTWLAREAW
                     DRLRTAGAKWGIELEVVDELIDEKLAANREEARGRRFWTRMTLTAAGGGAALVGVVIL
                     VLLVIRANKTPDVPPENPGAAAAQAAPITPKVATAPAWWDVDLSVEMATAETKFDGLS
                     GASVLMKSEIPDERASGYERMIELVKSASGNEELLDVAARIFTGCHALEPDESAAGRL
                     RATLLALLPAADAPLPTQPEQYDLAYWAAETAAGALHREGAAADRTRKLADALSAALS
                     APFDPAFGRSANLHAARVRTTLAAYQQLTAAASKQPSAAALLYTHLAPRAIRFIDDEE
                     LTKAETSLLVAALPTADEDWHAYDEAIVRCTSSADPLNVLRLTDTLQRCTDKELVDTL
                     SRMLVLRAGVTPATWEKTDVVKAVRKALGAAGATAGSTAADRWLVLREAADAALARPA
                     PPTANQHELLSQTIELAHLTTMAMALAQGEAGFASFDAGMTESPELAQMTSRGSVVDE
                     PKATGSAASRSPRSLGPTDRRDLERFQAMLGGFARMQPVQRVTALRGLALLGDKTGDI
                     APQYAENAAKYLLFDKADDEHAEVLRILPAIRHWKRLRLAVADLVLQSRLTAAQQHEL
                     GRTLLARDIPPDASSGGSLHRLLLESVLLEAGEAASRAESPGGDSGEVFDAAARLLAE
                     TYRQRARLLSISGPPLAEAQTPSQALDLVLRPIAQSLHGSAEDASFVANLDHLRTAAA
                     FLAGDDLRQTVALQRLMVELSTRRVIRQRPQQAATARQIETESLAKISQPGS"
BASE COUNT          552 a         1094 c         1018 g          484 t
ORIGIN      
        1 gctggccgca ggccgccgag cggctccagc gtttggccct cgccctgccg agcgatcggc
       61 ggttcctgcg gcgggcgggc ctcgccacgc tgcaggtcgg ccgccatgcc gattcgctcg
      121 agcactggcg gaggctgctc gcgggccttt ccagcggctc ggacgactgg ctggaggcga
      181 agtattacca actcgtctgt ctgggggcga ccgaccgcgc aacagccgag aaagtctgga
      241 aacaattcaa gctgcttttt cccgaagtga aatccgaagt gtggaaggag aagtttgcgg
      301 agttggagac cgagttttct tagccggcag caaacctgct gccggagaac cgtcaatgga
      361 ttggccactc gcggcccgcc gggaccaggt ttggtcccgg ctaaaagacg cgtaaacgac
      421 gtaatgcctc ccaagcccac cgccgcgcct cttgatcccg acctgctggc cgcgtttcgc
      481 cagcaggggg cggcaatcgt tgcgcaggag cgcggcctta cggccgtatg ccgcgtgaag
      541 ctcgcccggc tggcgggttc gctaggaatt cccgacgagc agatcgacgc cgcgatccgg
      601 gcactgcatg aatcggggcc caaagcgccg cccaatccgc tcgcagagaa attccgcgac
      661 cgcctccgca aagacctggc cggcaaaacg cgggcgatca ttggccccac gatcgaagcc
      721 gagatcatcg ccgccgcggc ccgcaagtac agcctcgacg agcccacctc gcggcaggtg
      781 ctcggcgaag tggccgaaga aatcggcgtc acgcggatta ccgccaccga cgcgatcgac
      841 agcctcgcga cgcagatcga acagaccgtg ggcgagagca catggctggc ccgcgaggcc
      901 tgggaccgcc tccgcaccgc cggggccaag tggggcattg agctggaggt ggtcgatgag
      961 ctgatcgacg agaagctcgc cgccaatcgc gaagaggccc gtggacgccg cttctggacg
     1021 cgaatgacgc tcaccgctgc cggcggcggc gcggcgctgg tcggcgttgt gattctcgtg
     1081 ctgctggtca ttcgggcgaa caaaactccc gacgtgccgc ccgaaaatcc cggtgctgcc
     1141 gccgctcagg ccgcgccgat caccccgaag gtcgccactg cgcccgcctg gtgggacgtc
     1201 gatctctcgg tcgagatggc gaccgcagaa acgaagttcg acggcctttc gggcgcatcg
     1261 gtgctcatga agtcggaaat cccggacgag cgcgccagcg gctatgaacg gatgatcgag
     1321 ctggtgaaat ctgccagcgg caacgaggaa ctgctcgatg tcgccgcacg gatcttcacg
     1381 ggctgccatg cgctggagcc ggacgaatcg gcagccgggc gactgcgggc gacgctgctc
     1441 gcgctcttgc cggcggccga tgcgccgctg ccgacgcagc ccgagcaata cgatttggct
     1501 tattgggccg ccgaaaccgc tgccggagcg ctccaccgtg aaggggccgc tgccgaccgc
     1561 acgcgcaagc tcgccgacgc gctgtcggct gcactttccg cccctttcga tccggccttc
     1621 ggtcgatctg caaacttgca tgccgcccgc gttcgcacca cgctcgccgc ctatcaacag
     1681 cttaccgccg cagccagcaa acagccttca gccgctgccc tgctctacac gcaccttgct
     1741 cctcgagcca tccgcttcat cgacgacgaa gaactgacga aagccgagac gagcctgctc
     1801 gttgccgccc tccccaccgc agacgaggac tggcacgcct atgacgaggc aatcgttcgc
     1861 tgcacctcgt cggctgatcc gctgaacgtg ttgcggctga ccgacacgtt gcagcgctgc
     1921 acggacaagg aactggttga tacgctgtcg cggatgctcg tgctgcgggc tggcgtcacg
     1981 cccgctacgt gggaaaaaac tgacgtcgtc aaagccgttc gcaaggcgct gggtgcggcc
     2041 ggagcaacgg ccggctccac ggcagccgac cgctggctgg tccttcgcga ggcagccgac
     2101 gcggccctcg cccggcccgc gccgccaacg gccaatcagc acgagctttt gagccaaacc
     2161 atcgagttgg cccatttgac gaccatggcc atggcgctgg cccaaggaga agcaggattc
     2221 gcgtcgttcg acgctggcat gaccgagtcg cccgaattgg cgcaaatgac ttcccgcggc
     2281 agtgtggtgg acgagccgaa agcgaccggc tcggccgcca gccgctcgcc gcgctcgctc
     2341 ggccccaccg accgccgcga cctggaacgg ttccaggcga tgctaggcgg ctttgcccgg
     2401 atgcagccag tgcagcgcgt gacggccctc cgcggtctgg cgctgctggg cgacaagacc
     2461 ggcgatatcg cgccacaata cgcggaaaac gcggcgaaat acctgctgtt cgacaaggcc
     2521 gacgacgagc acgccgaagt gctgcgaatc ctgcccgcca tccgccactg gaagcggctg
     2581 cgcctggcgg ttgccgacct ggtattgcag tcgcgattga ccgccgcgca gcagcatgag
     2641 ctcggccgga cgctcctggc ccgcgacatt ccgcccgatg cttcgtcggg cggtagcctg
     2701 caccgcttgc tcctggagag cgtgctgctg gaagctggcg aggcggcttc ccgcgccgaa
     2761 tcgcccggcg gcgattccgg cgaggtcttc gacgcggctg ctaggctgct ggctgagacc
     2821 tatcgtcagc gggcgcgcct cttgagcatc agcggaccgc cgctcgccga ggcacaaacc
     2881 ccgtcacagg cgctggacct cgtgctgcgg ccgatcgccc agtcgctcca cggctcggct
     2941 gaggatgcaa gcttcgtagc caacctcgac catcttcgca cagccgcagc ctttctggcg
     3001 ggagacgact tgcgacaaac ggttgccctg cagcggctga tggtcgagct ttcgacccgg
     3061 cgcgtcatcc gccagcgtcc gcagcaggcc gccaccgccc gccagatcga aaccgaatcg
     3121 ctcgccaaaa tcagccagcc tggcagca
//
LOCUS       DASUGB010001176         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_272422, whole genome shotgun sequence.
ACCESSION   DASUGB010001176 DASUGB010000000
VERSION     DASUGB010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272422"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VFV87_22310"
     CDS             <1..1366
                     /locus_tag="VFV87_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106577.1"
                     /translation="LGQMDSTSGGPPSKVRALLALPAIFLLINLSLATWRSSQITDLT
                     AGRSAAEACAAEQVSGPLRATCASFRLAVIGLKMSVLLVIIKLTAFGLSLASARLTWS
                     GKVPLMPAAKFAYYVSVLGSFANILLSAGLYAVCTQGTLMRLFDVSYLEFDFAMVGFM
                     MLVSAYSIWKIIDAQPLFPETSRMQVIGKAVNPDEQPALAAIVQRISEPLQSPIPNTV
                     IYGLEPTVRCTRRPVDLDGTEISGLTLFVSLPLCRLLSEGELISLITGELARCELVSP
                     DWEIWIERTYARWSVTAQSDRAAFFNLLWFWMEQWSTWSMILSLWCIQRSVVIAGREN
                     AAAGLAISSLVQTKWPSYLNHVQLALREGTIDAARQDVNLSEGFATMMNEKADELVAT
                     YWQTDGPNTGLPSLIESLQADPVAISQRIASEPTERASDILVGLERLELELSADQMKR
                     IFFT"
     gene            complement(1380..>3186)
                     /locus_tag="VFV87_22315"
     CDS             complement(1380..>3186)
                     /locus_tag="VFV87_22315"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5106578.1"
                     /translation="GGRKFNITLTGSNVNAAAGKAKLKTLQQLHHTGQSYQRDDIPAK
                     VDGSLKWAVDVTLPNMVHARNVKPPFVGATLTSFDESSVNKLPGFLKVMRKGNYLAVV
                     CEREEQAIRAARQLKASWQKPATPPLPHSDELFTYMRSAAPTSASQPTVTGDPAAAFT
                     NAATVVEAEYEVPFQGHTAFAGAHATADPTNGLMTVYSNDMKSYSMRTCVADFLAMPH
                     DRVRVVAMSGPQGYGRSAAEEAACEAAWIAKELGRPVRMQWMRDEETAWDTKGPAYVV
                     KMRGALDAQGAVTAYDYNARACDYNHIGYNEPDTILIAQLMGIRPPKPAAGRASMPSD
                     VYVIPNRRMAGDVVGLPMIWETPIRTGNLRDPDGPQVTFASESFIDELAAAAKADPLD
                     FRLRLLTASTTDDSGFRRARSIATLKAAAEAFGWERRAARKPATGNILTGRGIAYSFR
                     SQSIAVQIAEVEVNRQTGHVWAKRLVCAYDCGLVVNPTSLKRAAECGMLHSLSRALHE
                     EVRFDTEKVISTDWVSHPTLRHSDIPASIDIVLVNGDPNPQRPDLAAYGGGEAACKPL
                     LAAIANAIYDATGVRLRRVPFRDARVLAALRQARA"
BASE COUNT          561 a          810 c         1154 g          661 t
ORIGIN      
        1 tttggggcaa atggatagca ccagcggggg gccaccgtcg aaagtccggg cgctgctggc
       61 ccttcctgcc atcttcctgc tcatcaatct gtcgctggca acctggcgtt catcgcaaat
      121 cacagacctg acagccggca ggagtgcggc cgaggcttgt gcggcggagc aagtcagtgg
      181 accactgcgg gccacctgcg ccagctttcg tctagctgta atcggtttga agatgagcgt
      241 gttgctggtg atcatcaaac tgactgcctt tggcctttct ctcgcttccg cccggctgac
      301 atggagtggc aaggtgcctc ttatgcctgc cgcaaagttc gcctactacg tttctgtctt
      361 aggatcgttc gccaatatct tgctttcggc agggctgtat gcggtttgca cacaaggaac
      421 cttgatgcgg ctttttgacg tctcgtatct cgagtttgac ttcgcgatgg tgggattcat
      481 gatgctggtg tccgcctact caatctggaa gattatagat gcacaaccgc tatttcctga
      541 aaccagtcga atgcaggtga tcggcaaggc ggtgaatccc gacgagcagc cggcactggc
      601 ggcaattgtt cagcgaattt cagagccact tcagagtccg attcccaaca cggtcattta
      661 tgggttggaa ccgaccgtac gttgcacgcg gcggcctgta gatctggacg gaacggagat
      721 tagcggccta acgctgttcg tttcccttcc gctgtgccgg ctactgtcgg aaggcgaatt
      781 gatttcactg atcacgggag agcttgcgag atgtgaactc gtctcgcccg attgggagat
      841 atggatcgag cgcacatatg ccaggtggag cgttactgcc caaagtgacc gggcggcgtt
      901 cttcaactta ctttggttct ggatggagca atggagcacc tggtccatga ttctctcgct
      961 atggtgcatc caacgatccg ttgtcatcgc aggacgagag aacgcagctg ccgggctggc
     1021 aatcagctcg ctggtccaaa caaaatggcc gtcgtatcta aatcacgtgc aattggcact
     1081 gcgtgaggga acgattgatg cggcacggca ggacgtcaat ctaagcgaag ggttcgcgac
     1141 catgatgaac gaaaaagccg acgaactggt ggctacctat tggcaaacag acgggccaaa
     1201 tacgggactg ccatcgttga tcgaatcact gcaggcggat ccggttgcta ttagccagcg
     1261 cattgccagt gaaccaacgg agcgcgcatc cgatatcctc gtcggactgg agcgccttga
     1321 gttagagctc agcgccgacc agatgaagcg gatcttcttt acgtgagcta cagcgtcgct
     1381 tacgcacggg cctggcgcag ggcggcgaga acgcgggcgt cgcggaacgg gacgcggcgg
     1441 aggcggacgc cggtggcatc gtagatggcg ttggcgatgg cggcgagcag gggtttgcag
     1501 gcagcttcac cgccgccgta agcggcaagg tcagggcgct gcggattggg gtcgccattg
     1561 acgaggacga tgtcgatgga ggccgggatg tcggagtggc ggagcgtggg gtgcgagacc
     1621 caatcggtgc tgatcacctt ttcagtgtcg aagcggacct cttcgtgaag agcgcggctt
     1681 aaggagtgga gcatgccgca ttcggcggcg cgcttgaggc ttgtgggatt cacgacgagc
     1741 ccgcagtcat aagcgcagac gagacgtttg gcccagacgt ggccggtttg gcggttcact
     1801 tcgacttcgg cgatttgcac ggcgatggac tggctgcgga aggagtaagc gatgccgcgg
     1861 ccggtgagga tgttgcctgt ggcgggcttg cgtgcagcgc ggcgttccca accgaaggcc
     1921 tcggcggcag ccttgagcgt ggcgatggag cgggcgcggc ggaagccgct gtcgtcggtg
     1981 gtggaggccg tgaggaggcg gaggcggaag tcgagcgggt cggctttggc ggcggcggcg
     2041 agttcgtcga tgaaggattc ggaggcgaag gtgacctgcg ggccgtcggg atcgcggagg
     2101 ttaccggtgc ggatgggcgt ctcccagatc atggggaggc cgaccacgtc accggccatt
     2161 cgtcggttgg ggatgacgta gacgtcggag ggcatggagg cgcgtccggc ggcgggcttg
     2221 ggcgggcgaa tgcccatcaa ttgagcgatg aggatcgtgt cgggttcgtt gtagccgatg
     2281 tggttgtagt cgcaggcgcg ggcgttgtag tcgtaggcag ttacggcgcc ctgggcatcg
     2341 agtgcgccgc gcatcttgac gacgtaagcg gggcccttgg tgtcccacgc ggtttcttca
     2401 tcgcgcatcc actgcatgcg aacggggcgg ccgagttctt tggcgatcca tgcggcttcg
     2461 caggcggctt cttcggcggc gctgcgtccg tagccttgcg ggccggacat ggcgacgacg
     2521 cggacacggt catgcggcat ggcgaggaaa tcggcgacgc aggtgcgcat gctgtacgac
     2581 ttcatgtcat tggagtagac ggtcatcagg ccattggttg ggtcggcggt ggcgtgggca
     2641 ccggcgaagg cggtgtgccc ttgaaagggg acttcgtact cggcttcgac gactgtggcg
     2701 gcgttggtga atgcggcagc ggggtcgccg gtgacagtgg gctgtgaggc ggaggtgggc
     2761 gcggcggagc gcatgtaggt gaagagttcg tccgagtgag ggagcggcgg agtggcgggc
     2821 ttctgccagg aggctttgag ttggcgggcg gcgcggatgg cctgctcttc gcgttcgcag
     2881 acgacggcga ggtagttgcc cttgcgcatg actttgagga agccgggcag cttgttgacg
     2941 gaggactcat cgaacgaggt gagggttgcg ccgacgaagg gcggcttcac gttgcgcgcg
     3001 tggaccatgt tggggagcgt gacatcgacg gcccatttga gggagccatc gaccttggcg
     3061 ggaatgtcgt cgcgctggta ggactggccg gtgtggtgga gctgctggag agttttgagt
     3121 ttggctttac cggcggcggc gttgacgttg ctgccggtga gggtgatgtt gaatttgcgg
     3181 ccgccg
//
LOCUS       DASUGB010001177         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1383409, whole genome shotgun sequence.
ACCESSION   DASUGB010001177 DASUGB010000000
VERSION     DASUGB010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383409"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1114)
                     /locus_tag="VFV87_22320"
     CDS             complement(122..1114)
                     /locus_tag="VFV87_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5106579.1"
                     /translation="MFARCGFAVFMAMVALGFAELAQAGPAYADPDKADADFAYQGEY
                     VGKLKSDDGNDVTVGLQIIALGQGKFHAVGYGGGLPGDGWDMENKKEADGELKDGAAT
                     FEGDEAKASVKDGVATIMTKDGKELGKLDKVSRSSPTLGAKPPEGAVVLFDGKSADAF
                     DGGKLSDDGLLMQGCTSKQKFGSCKLHIEFRLPYMPEDRGQARGNSGIYVGGRYECQM
                     LDSFGLTGENNECGGLYSVKAPDVNMCLPPLAWQTYDIDYTAAVFEGDKLVKNPRVTI
                     SHNGVVIHKDVELPGERSTTAAPNKPGPEPGPVYLQDHGNPVRYRNVWVVEGGK"
     gene            1490..1900
                     /gene="yajC"
                     /locus_tag="VFV87_22325"
     CDS             1490..1900
                     /gene="yajC"
                     /locus_tag="VFV87_22325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5106580.1"
                     /translation="MSDLLVVFSFLPLLLAQAGGGAPKGGGGGGGGDLFGSSMFPIMI
                     IVTALMFYLLMMRPEQRKRKEMQKLLDALKKNDHVVTTGGIVGTVLVANEGSKFVTLR
                     IDDATGAKVRILRSAIMHVGAVEEAESDAKVGEK"
     gene            1956..>3404
                     /locus_tag="VFV87_22330"
     CDS             1956..>3404
                     /locus_tag="VFV87_22330"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106581.1"
                     /translation="MNPSSPLLLAATGYRLINYDFPALFSGEGSAGFSSLWLVALALL
                     AIAIGAGFVVANSVRLKDYGWKFALILGSFVLSLLLVLFGEYRLGVDLKGGVILVYEI
                     DKAETAALHPQGRSDDWSMNALLQVLRNRLNPDGLKEIVIRPFGPEQIEIIVPETDPG
                     EVNEIKEKIITGGVLQFMVVASQAKDSDLLEVAREQAAQKRLEDRIRRDVNNESGERV
                     GYWAKLGREQSSQLAGGNEKAPFRELDSLREGFLRDARTGEIIDLTPQQRFDFSSSSA
                     NFQDFLDRRGTREVDVLLVYDAVYDLRGDDLRFVNKFRDPKTGSWAISFTMKGEGIFK
                     MGTLTQENLHRKLAIVFDNTLLSAPVIQSRIDENGQITGRFTEEEVDFIVGILRSGSM
                     PVVLQKNPISENRIGSILGRDTIEQGSYSIVVSLFLVLGFVLVYYRFSGLVACFALIL
                     NILLTLGFMILLRAPLTLPGLAGLVLTVGMSVD"
BASE COUNT          597 a         1121 c          977 g          709 t
ORIGIN      
        1 tctcggggtc gctcacaaca cgcccctttc actccctcgc ttacgctttg ggttggtgtg
       61 ctacctcgct tacgcgtcgg gtcggtgagc cgcaccactc acccactcac cactcaccca
      121 ctcacttgcc gccctccacc acccacacat tccgataccg caccgggttg ccatgatcct
      181 gcagatagac gggccccggc tccgggccgg gcttgttggg ggcggcggtg gtcgaacgct
      241 ctccgggcag ctcgacatcc ttgtggatca ccacgccgtt atgcgaaatc gtcactcgcg
      301 ggttcttcac gagcttgtcc ccttcgaaaa cggctgccgt gtagtcgatg tcgtacgtct
      361 gccacgccag gggcggcagg cacatgttga cgtcgggggc cttcaccgaa tacagcccgc
      421 cgcactcgtt gttctcaccc gtcaggccaa acgagtcgag catctggcac tcatacctcc
      481 cgccgacata tatcccgctg ttgccgcggg cctggccgcg gtcttccggc atgtagggga
      541 ggcggaactc gatgtgcagc ttgcagctcc cgaacttctg cttgctcgtg cagccctgca
      601 tgagcaggcc gtcgtcgctc agcttgccgc cgtcgaacgc gtcggccgat ttgccgtcga
      661 acagcacgac cgcgccttcc ggcggcttgg cgccgagcgt cgggctgctg cggctgacct
      721 tgtcgagctt gcccagctcc ttgccgtcct tggtcatgat cgtggcgacg ccatccttca
      781 cgctcgcctt cgcctcgtcc ccctcaaagg tcgccgcgcc gtccttgagc tctccgtcag
      841 cttccttctt gttctccatg tcccaaccat cgcccggcaa gccgccgcca tagccaaccg
      901 catggaattt gccctgccca agcgcgatga tctgcaatcc cacggtgacg tcgttgccat
      961 catcgctttt cagcttgccg acgtattcgc cctggtaagc gaaatcggca tccgccttgt
     1021 ccggatctgc ataagccggc cccgcctgag ccagctcggc aaatcccagc gcgaccatcg
     1081 ccataaaaac cgcgaaacca caacgggcaa acatcagaga cgctcctggc agagaggaat
     1141 tcaccacatt catttcctcc ggccattgtg ctgccgaagt cacgcgtatg caagaatctg
     1201 tgacgcccct cccttcccac cgactgcgtc cgcgctgcaa ggcgctacgg acggcccccg
     1261 aggcgcaatg ggaatttggg tctgcgccct gccaggttca tccgcccttg tcgcttgggg
     1321 cgcaaggcct tatgatcggc ggagttaggc aagtttagcc ggccctgcgc cgcgaagctg
     1381 agggcgctgc tgcgccacac cgcgcagggc agcctgtcgt ttagggctgt ccgtcgttag
     1441 ggctgtccgt cgttaggcat tggccggtct tggcctggga gcgacttcgt tgtccgatct
     1501 tctcgtggtc ttttccttcc tgccgctcct gctggctcag gcggggggcg gcgcgcccaa
     1561 aggcggcgga gggggaggcg gtggcgatct gttcgggagc tcgatgttcc cgatcatgat
     1621 catcgtcacc gcactcatgt tctacctcct gatgatgcgg cccgagcagc gcaagcgcaa
     1681 ggaaatgcag aagctgctcg atgccctcaa gaagaacgac cacgtcgtga ccactggcgg
     1741 catcgtcggc accgttttgg tggccaacga aggctcgaag ttcgtcaccc tgcgcatcga
     1801 cgacgcgacc ggcgccaagg ttcgcatcct ccgcagcgcc attatgcacg tcggcgccgt
     1861 tgaggaagcc gagagcgacg ccaaagtcgg cgaaaagtaa aacgcgaaag cccatccaac
     1921 ggaattcatc ccttctggtt acggacctga ccgtcatgaa tccttcctct ccgctcttgt
     1981 tggcggccac gggataccgc ctgatcaact atgatttccc ggccctcttc agcggcgaag
     2041 gctctgccgg tttttcctcg ctctggctcg tcgccctggc ccttttggcg atcgccatcg
     2101 gcgccggttt tgtcgtggcc aattccgtgc ggctgaagga ctatggctgg aagtttgctc
     2161 tgatccttgg ctcgttcgtg ctcagccttc tgctcgtgct gtttggcgag taccggctgg
     2221 gggtcgatct gaagggcggc gtgattctcg tctatgagat tgacaaggcc gaaacggccg
     2281 ccttgcaccc gcaggggcgc tcggacgatt ggagcatgaa tgccctgctg caggtgctgc
     2341 ggaaccggct caatcccgac gggctgaaag aaatcgtcat ccggccgttt ggcccggaac
     2401 agatcgaaat cattgtccca gaaaccgatc ctggcgaagt caatgagatc aaagagaaaa
     2461 tcattaccgg cggcgtgctg cagttcatgg tcgttgccag ccaggccaaa gactccgatc
     2521 tgctggaagt cgcccgcgaa caagccgccc agaagaggct ggaagaccgc atccgccgcg
     2581 atgtcaacaa cgagagcggc gagcgcgtgg gttactgggc caagctgggg cgcgagcaat
     2641 ccagccagct tgccggcggc aacgaaaagg ctccctttcg ggagttggac agcctgcgag
     2701 aaggcttcct tcgcgatgcc cgcaccggtg agatcatcga tctgaccccg cagcaacgct
     2761 ttgacttttc atccagcagc gcgaattttc aagattttct cgatcgccgc ggcacgcgcg
     2821 aggtcgatgt cctcctcgtc tatgacgccg tctatgacct ccgcggcgac gacctgcggt
     2881 tcgtgaacaa gtttcgcgat ccgaagactg gaagttgggc catctccttc accatgaagg
     2941 gcgaaggcat cttcaagatg ggaaccctga cgcaggagaa cctccatcgc aagctggcga
     3001 tcgtcttcga caacacgctt ctctcggccc ccgtcattca gagccggatt gacgaaaacg
     3061 gccagatcac gggacggttc acggaagaag aagtcgattt catcgtcggc atcctccgct
     3121 cgggcagcat gcccgtggtg ctgcaaaaaa acccgatcag cgagaaccgc atcggctcga
     3181 ttctgggccg cgacacgatc gaacaaggca gttactcgat cgtcgtgtcg ctcttcctgg
     3241 ttctcggctt cgtgctcgtc tattaccgct tctccgggct ggtcgcttgc tttgccctga
     3301 tcctgaacat cctgctgacg ctgggcttca tgatcctcct gcgtgcgccc ctcacgcttc
     3361 ccggcctggc gggcctggta ctcaccgtcg gcatgtcggt tgat
//
LOCUS       DASUGB010001178         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1383427, whole genome shotgun sequence.
ACCESSION   DASUGB010001178 DASUGB010000000
VERSION     DASUGB010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383427"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFV87_22335"
     CDS             <1..915
                     /locus_tag="VFV87_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5106582.1"
                     /translation="DVVMDAMMRLNDRIAGTHQVGGVDYGFRELDGKTAGLHNSELVI
                     LAARPSMGKTALAMNIAENVALQQNVPVLFVSLEMSSLELADRLLCSVARVNGHRLRN
                     GTVSQDDRLKLVEKAEVLSKIPLFVDDSPSRTMTEIAAAARRVKRRQGSLGLIVIDYL
                     QLIEPDNSKDPRQEQVAKIARRLKGLAREMKVPVLCLAQLNRQAEASGGNIPRLSHLR
                     ESGAIEQDADVVMFVHREEYYQRGEDRAAHEGQAQIIIAKQRNGPVGEVELEWLRDFT
                     RFQDKVPERLKEFDQYNDRQMVDPAAGF"
     gene            1010..1714
                     /locus_tag="VFV87_22340"
     CDS             1010..1714
                     /locus_tag="VFV87_22340"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX5106583.1"
                     /translation="MHPPEIVVIDTPHPCSYLPGRTARLPYRHPLEMVPPEVFDERLA
                     EGDRRSGVFLYRTKCPACRACEPIRLDLTGFRPNDTQRRIQRRGDALLKVQIARPIID
                     TQRIDLFNAHRDVRGLARDDEPIDEEGYADFLTETCCETLELTCWHDGRLVACAIADA
                     GRASLSAVYTFFDPHFTLLSLGTYCVLREAELCRQTDRRYLYLGFYIAASPHMAYKAS
                     FHPHQRRIDGQWVDFA"
     gene            1928..>2182
                     /locus_tag="VFV87_22345"
     CDS             1928..>2182
                     /locus_tag="VFV87_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106584.1"
                     /translation="MADRGCSWQVQSPLRAMVCAAAGLLILAPAEAALAQYPLQPSSN
                     ALPGPPDLQAPADPQVRQLVVGVQILGNSDTKDFDVQKHIH"
BASE COUNT          392 a          708 c          691 g          391 t
ORIGIN      
        1 gatgtcgtga tggatgcgat gatgcgcctc aacgaccgca tcgccggcac gcaccaggtc
       61 ggcggcgtcg attacggctt tcgcgagttg gacgggaaga cggcgggcct gcacaattcc
      121 gaactggtga tcctcgccgc ccggccgagc atgggcaaaa cggctttggc gatgaatatt
      181 gccgagaatg tcgctttgca gcagaacgtg cccgtgctgt ttgtcagcct ggaaatgtcg
      241 tcgctggaat tggccgaccg tttgctctgc agcgtggccc gcgtgaacgg ccatcgcctg
      301 cggaacggca cggtttcaca ggacgaccgc ctgaagctcg tcgagaaggc ggaagtgctt
      361 tcgaaaatcc cgctgttcgt cgatgacagc cccagccgca cgatgaccga gatcgcggca
      421 gccgcccggc gcgtgaagcg ccggcagggg tcgctgggcc tgattgtgat cgactatctg
      481 cagctcatcg agccggacaa ttccaaggac ccgcggcaag agcaggtcgc caagatcgcc
      541 cgccgcctca agggcctggc ccgcgagatg aaagtgcccg tgctgtgcct ggcgcaactc
      601 aatcgccagg cggaagccag cggcggcaac atcccgcggc tcagccacct gcgcgaatcg
      661 ggggcgatcg agcaggacgc cgacgtcgtg atgttcgtcc accgggagga gtactaccag
      721 cgtggcgaag accgggccgc tcacgaaggt caggcccaga tcatcatcgc caagcaacgc
      781 aacggtcccg tcggcgaagt cgagctggag tggctccgcg acttcacccg tttccaggac
      841 aaggtgcccg agcgactcaa ggagttcgac cagtacaacg accggcagat ggtcgatccc
      901 gcggcggggt tctgaccagt gcggaatgcg gaatgtagaa tgcggagtgg acgcaggtgc
      961 cacttgctgg tcactccgca ttccgaattc cacattccgc attcgtccct tgcatccgcc
     1021 agagatcgtc gtcatcgaca cgccgcatcc gtgctcctat ctgccgggac gcacggcccg
     1081 cttgccgtac cggcatccgt tggagatggt gccgccggaa gtgttcgacg agcgattggc
     1141 cgagggggac cgccgcagcg gagttttctt gtaccggacg aagtgccccg cgtgccgggc
     1201 ctgcgagccg atccgcctcg atttgaccgg cttccggccg aatgacacgc agcggcggat
     1261 tcagcgccgg ggcgatgccc tgctcaaggt acaaatcgcc agaccgatca tcgacaccca
     1321 gcgaattgac ctttttaacg cccaccgcga cgtacgtggc ctggcccgcg atgatgagcc
     1381 gatcgacgag gagggatacg ccgacttcct gacggagacc tgctgcgaga cgctggagct
     1441 gacgtgctgg catgacgggc ggctggtggc gtgcgcgatc gccgatgccg gccgcgcgtc
     1501 gctgtcagcg gtctatacgt ttttcgatcc gcatttcaca ctccttagcc tgggcaccta
     1561 ttgcgtcctc cgcgaagccg aactctgccg gcagacggat cgccgctacc tgtatctcgg
     1621 cttctacatc gccgcctcgc cgcacatggc ctacaaggcg agctttcatc ctcaccagcg
     1681 ccggatcgat ggccagtggg tcgatttcgc ttgagctgct tacgcgagct agcggcggca
     1741 gcaccggctg ggatgcctgc cgcggctcgc gcacgaattc gccttgccgc tgtcacgggc
     1801 tttgagtatt gatacgcggc ctttgccggg gctttgcgcc cattggcctg cgcgcgcagc
     1861 ttggcaagca ccgtggcagg cccggcaaac cctaatctga aacgaacttt cgcgggaatc
     1921 ctcgtcgatg gcagatcgcg gctgctcgtg gcaagtgcaa agcccactcc gcgcgatggt
     1981 ctgcgcggcg gccggtttat tgattctggc gcctgccgag gcagcactcg cccagtatcc
     2041 gctccagccg tcttccaatg ccctgcccgg cccgccggac ctgcaggcac cggctgatcc
     2101 gcaagtccgc cagcttgtcg tcggcgtgca gattctcggc aacagtgaca cgaaggactt
     2161 cgatgtccag aagcacatcc ac
//
LOCUS       DASUGB010001179         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1937666, whole genome shotgun sequence.
ACCESSION   DASUGB010001179 DASUGB010000000
VERSION     DASUGB010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937666"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFV87_22350"
     CDS             <1..627
                     /locus_tag="VFV87_22350"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5106585.1"
                     /translation="AAGADAVKVGIGPGSICTTRVISGVGVPQITAIYEAAQAAKASG
                     TPIIADGGIRYSGDMTKAIAAGAHCVMIGGLFAGLAESPGKTILYQGRTFKTYRGMGS
                     LGAMVKGSSERYRQSGESGGTGKLVPEGVEGRVPFKGPLSDFVYQLVGGLRAGMGYCG
                     TRTIEELRTDTRFIQVSAATVRENHPHDVAITQEAPNYSPEYHDGSGE"
     gene            698..1951
                     /locus_tag="VFV87_22355"
     CDS             698..1951
                     /locus_tag="VFV87_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106586.1"
                     /translation="MPAFGQIIEPARFVADPEARPIAEASSVAVDGAPLRWKSKPQSV
                     AQAQFSTDAPQPISAEEPARVGWNVTRVDLSVKPAQFTQPAPASDPFSDPFGDRQTVR
                     SVSGEIELQPTATEAGVEELPAPLPISGPRNASPPRPVERTPSPPAEMFEAAQPAPSI
                     PTPESDAERYAPGRPNVPCNRIYNDRNCCDVDSACNAFRNALLSSSLRHISLDITPRF
                     MPDLTEAEDEAERLKKLNILEIRPWKSRTGELLATGRMANFDNGSLVVVSDEGEEVAR
                     IPWHQLGEDELCYVNAWWRLPAECTLGGLRTAERNWIPSTFAWHASALCHKPLYFEDV
                     QLERYGHTTGPYSQPFVSGAHFFLNIAALPYNMAIHPPQECIYALGYYRPGNCAPWMI
                     QPVPLSLRGAIAEAGVVVGGVYIIP"
     gene            2162..3505
                     /locus_tag="VFV87_22360"
     CDS             2162..3505
                     /locus_tag="VFV87_22360"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106587.1"
                     /translation="MSRRELLRIGGSGLMGLSLSRLLAASENPSVTTRPKADSLIILM
                     LNGGPSHLDMWDMKPEAPDNIRGEFKPISSSLAGVPVSEHLPKLSQQMHRACLVRSMN
                     HSVNNAHAMAVYTAMTGHDRGDANVAIGNGGSDNPSPGAVLARVRPSMGDTVPHVCLP
                     YMTKEGAGGPPQPGFWGGYLGRAYDPLWVLKDPNAADFSVPEFTLASDVSIERLTNCG
                     QLLTSLNQTMDAVQGRTAFDTMNRFQQRALDVLTSETVRNAFRLEQEPDPVRESYGRN
                     IYGQSVLLARRLIESGTRVVTISWAPDANATWDTHGGNFKKLKGTLLPQFDAACGTLL
                     QDLADRGRLERTLVAVIGDFGRTPKINNNDGGRDHWNWCYTVMLAGGGIRPGYAFGAS
                     DRIGAFPADRPVTPGDLVATIYSLLGVDPSQELYDAQDRPHRVIPKGDVIHELIA"
BASE COUNT          643 a         1190 c         1149 g          616 t
ORIGIN      
        1 gccgccgggg ccgacgccgt gaaggtcggg attggtccgg ggtcgatttg cacgacccgc
       61 gtgatcagcg gcgtgggcgt gccgcagatc acggccattt acgaagccgc ccaagccgcg
      121 aaagcgagcg gcacgccgat catcgccgat ggcggaatcc gttactcagg agacatgacc
      181 aaggcaatcg ccgccggcgc acattgcgtg atgatcggcg gcttgtttgc cggcctggcc
      241 gaaagcccgg gcaaaaccat cctctaccag ggccgcacat tcaagacgta tcgcggaatg
      301 ggcagcctgg gggcgatggt gaagggatcg agcgaacgat acagacaatc cggcgagagc
      361 ggggggaccg gcaagctggt tccggaggga gtcgagggac gcgtgccttt caaagggcca
      421 ctctccgact tcgtctatca gcttgtcggc gggctcagag ccgggatggg atattgcggg
      481 acccgaacga ttgaggaatt gcgtacggat acgcggttca tccaagtctc cgccgccacc
      541 gtgcgggaga accaccccca tgacgttgcg atcacgcagg aagcgccgaa ctacagcccc
      601 gagtatcacg acgggagcgg cgagtaaggg ttcaaggctc tgggttcagg ggtcaggagt
      661 cagggcgctg ggatttgcgc tggcggttgg cattgccatg ccagctttcg gccaaatcat
      721 cgagccggct cgatttgttg cggatcctga ggcgcggccg attgctgaag cgtcttctgt
      781 tgctgtggat ggcgccccgc tgcgctggaa atccaagccg cagtctgtcg cccaggcgca
      841 attcagcacg gatgcgccgc agccgatctc ggctgaagag ccggcgcggg tgggctggaa
      901 cgtcacccgc gtcgatttgt ctgtgaaacc ggcccaattc acccagcccg cacctgcttc
      961 cgatccgttc agtgatccct ttggcgaccg gcaaacggtt cgcagtgttt cgggcgaaat
     1021 cgagctgcag ccgaccgcga cggaagcggg cgtcgaggag ctccccgctc ctctgccgat
     1081 cagcgggccg cgaaacgcca gcccgccgcg cccggtcgag cgaacgccca gcccgccggc
     1141 cgagatgttc gaagccgctc agcctgcgcc gtcaattccc acaccggaaa gcgacgccga
     1201 gcgctacgct cccgggcgtc cgaatgtccc ttgcaatcgc atctacaacg accgcaactg
     1261 ctgcgacgtc gattcggctt gtaatgcctt tcggaatgcg ttgctctcca gctctctgcg
     1321 acacatctcg cttgatatca cgccccgttt catgcccgac ctgaccgaag ccgaggacga
     1381 ggcggaacgg ctgaaaaaac tgaatatcct ggagattcgc ccgtggaaga gtcgcacggg
     1441 cgagttgctg gccacgggcc ggatggccaa ttttgacaac ggcagtctgg tcgtggtgag
     1501 cgatgagggc gaggaggttg cccgcattcc ctggcaccag cttggcgagg acgagctatg
     1561 ctacgtcaac gcctggtggc ggctgccggc ggagtgcacc ttgggcggcc tgcggacggc
     1621 cgagcggaac tggattccca gcacattcgc ctggcacgcc tcggccctgt gccacaagcc
     1681 gctgtacttt gaggatgtgc agctggaacg ctacggccac acgaccgggc catacagcca
     1741 gccgttcgtc agcggggccc acttcttcct caacatcgcc gccctgccct acaacatggc
     1801 gattcatccc ccgcaggaat gcatctacgc cctgggctac taccgtcccg gcaactgcgc
     1861 tccctggatg atccaaccag tgcccctctc gctccgcgga gcaatcgccg aagccggcgt
     1921 cgtggtcggc ggcgtatata tcattcccta gtgtggggca gacgtccacg tctgcctgaa
     1981 ccgtcctcct gccggtactc cagttcggga gcgattggcg aagcaaacgg cgaggcccct
     2041 gcggcctcgc cgttttgcct ttcttgaagc ggctagccag ccaggcccga ttcgcgctac
     2101 actggcgttg ccttccagcg aacctcccgc catgcccgga tctgtcgcac caagaagttc
     2161 cttgtcccgc cgcgagctgc tgcgcattgg cggcagcggg ctgatgggac tctcgctgtc
     2221 gcggctgctc gcggcgtctg agaatccctc cgtcacgact cggcccaaag ccgattcgct
     2281 gatcatcctg atgctcaacg gcgggccaag ccatctggat atgtgggaca tgaagccgga
     2341 ggcgcccgac aatatccgcg gcgaattcaa gccgatcagc agctcgctcg ccggcgtgcc
     2401 ggtaagcgag catctgccga agctttcgca gcagatgcac cgcgcgtgtc tcgtgcggtc
     2461 gatgaatcac agcgtcaaca atgctcacgc gatggccgtc tatacggcga tgaccggcca
     2521 cgaccggggc gacgcaaacg tggcgatcgg caacggcggc agcgataatc cgtcgcccgg
     2581 cgcggtgctg gcccgcgtgc gacccagcat gggcgacacc gtgccgcacg tctgcctgcc
     2641 gtacatgacc aaggaaggcg ccggcggccc gccgcagccg ggattctggg gcggctatct
     2701 cggccgggcc tacgatccgc tgtgggtgct gaaggatccc aatgccgccg atttctcggt
     2761 gccggagttc acgctggcca gcgacgtctc gatcgagcgg ctgacgaatt gcgggcagct
     2821 tttgacgtcc ctgaaccaaa cgatggacgc ggtgcagggc cggacggcct tcgacacgat
     2881 gaaccgcttc cagcagcggg ccctggatgt gctgacgagc gagaccgtcc gcaacgcctt
     2941 ccgcctggag caggagccgg acccggttcg cgaaagctac ggccgcaata tctacggaca
     3001 atcggtcctg ctcgcccggc ggttgatcga atctggcacg cgcgtggtca cgatcagctg
     3061 ggcgccggac gccaacgcca cctgggacac ccacggcggc aacttcaaga agctcaaagg
     3121 cacgctgctg ccccagttcg acgcggcgtg cgggacgctg ctacaagacc tggccgaccg
     3181 cggccggctg gagcggacgc tcgtcgccgt gatcggcgat ttcggccgca cgcccaagat
     3241 caacaacaac gacggcggcc gcgaccactg gaactggtgc tacaccgtga tgctcgccgg
     3301 cggcggcatc cggcccggct atgccttcgg cgccagcgac cggatcggcg ccttccccgc
     3361 cgaccgcccc gtcacgcccg gcgacctcgt cgccacgatc tactcgctcc tcggcgtcga
     3421 tcccagccag gaactctacg acgcccagga tcgcccgcac cgggtgattc cgaaggggga
     3481 tgtgattcac gagctgattg cgtgagtcgc attgaccggt tcatcagcgt ctgatagcgt
     3541 cccgccgctt ttcgtccgaa tttctgccgg cgggatcatg cgatggatcg cctttttg
//
LOCUS       DASUGB010001180         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1662273, whole genome shotgun sequence.
ACCESSION   DASUGB010001180 DASUGB010000000
VERSION     DASUGB010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662273"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFV87_22365"
     CDS             <1..771
                     /locus_tag="VFV87_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5106588.1"
                     /translation="QFGVMEIDANQRIVGFEEKVPNPKTIPGDPEHCLGSMGIYVFAA
                     RFLFEQLCRDATRSDSKHDFGRNIIPALIDTHKVYAFPFRDENRKSDAYWRDVGTLDA
                     YFEANMDLVGIDPLLNLYDETWPVRTNQLNLPPPKFVFGSRGETDRAGRALDSIVCPG
                     TIVSGGLVERCILGPRVRINSYAQVSDSILFEGVDVGRHAKIQRAIIDKGVKVPPGMQ
                     IGFDLGLDRKRGLTISDGGIVVIAKGDGLEHMGDSPRG"
     gene            782..1642
                     /locus_tag="VFV87_22370"
     CDS             782..1642
                     /locus_tag="VFV87_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106589.1"
                     /translation="MDETRKEDDASAQGLPGATRSSTSDQDPQSIAGLEINLGDFTRT
                     QVLMRSLRLTLNNQIVVQVEEIASGVSEAGRKFKLGDVEQANLDVARLYAAFDQKTNQ
                     WESQARNLQQQMKAQAAKNPRSISIDKMNQMKAEQTAVRTRIRTAEVQFRRLHQGLEQ
                     AFTILQSQPPPATSAAPADLPPGFLARFEAAASADRPAVVRELFEIEAELKVSVTRGK
                     STGYEIRFSPTPAKGRLYFLTTTAQIIRLEQIGNSVVILDLVSGEETEMKLSDFVKRV
                     QAGASLLQPR"
     gene            complement(1798..2283)
                     /locus_tag="VFV87_22375"
     CDS             complement(1798..2283)
                     /locus_tag="VFV87_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106590.1"
                     /translation="MWTCVAIVVGVMLAVGGHGCGRHPYKPPPHEDAMKAKIDPSLPL
                     IELPMFDLGQPVTSANDDRSPQALGQTVPASDFSIRVDAKRPASHRGPPLPVVFRILR
                     RGATGELLITASGFLKPTLQEDGTVRYEGTCSRPVAFGASVLEIKLLEQSFLVRSAEV
                     R"
     gene            complement(2339..2743)
                     /locus_tag="VFV87_22380"
     CDS             complement(2339..2743)
                     /locus_tag="VFV87_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106591.1"
                     /translation="MTRSILSWLFVTATVLLTPIALHAGWSFTFAGNPMYGIKSIDLT
                     GMGPEGEDALLLQYLEVDEEWDEYGPGWTINAAPGSIPDPFEETMWSKTVDGPDANNQ
                     PPWDSTYPVGHYKPEIWDPGVVKTQYFFVTEM"
     gene            complement(2785..>3667)
                     /locus_tag="VFV87_22385"
     CDS             complement(2785..>3667)
                     /locus_tag="VFV87_22385"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5106592.1"
                     /translation="IVVLAIIGILFALLLPAVQAVRESGRRLECASNLRQLSLAVHQY
                     EQLHGVLPRAMRKNYSFHVALLPHLEQQSLFRQFDFSINQTDYQGNLQGVRVAIFQRP
                     SDASSRVVIRDWAATSYFGNCGSGSQRYGYNGVFGYRLHPHGGIDTLTSSSISDGLSN
                     TAMFSEVLAADGSYHWLRVYWLAPALGGKDQLDQFAQACANLPDSAPNSFTDNLWCGR
                     PWIRGGITGTLYNHVLGPNSPSCLNGASIDASAFSSASFHGSVVNVANADGSVQSVNQ
                     AIDLVQWRRLASRSGSP"
BASE COUNT          778 a         1074 c         1124 g          691 t
ORIGIN      
        1 caattcggcg tgatggagat cgacgctaac cagcggatcg tcggctttga ggagaaggtg
       61 ccgaacccca agacgattcc gggcgatccc gagcactgcc tgggctcgat gggcatttac
      121 gtcttcgccg cccgcttcct gttcgagcag ctttgccgcg atgcgacgcg ctctgacagc
      181 aaacacgatt ttgggcggaa catcattccc gcgctgatcg acacgcacaa ggtctatgcc
      241 ttcccgttcc gcgacgagaa ccgcaagagc gatgcgtact ggcgcgacgt gggcacgctg
      301 gacgcgtact ttgaggcgaa catggacctc gtggggatcg acccgctgct gaacctgtac
      361 gacgaaacgt ggcccgtccg caccaaccag ctcaacctgc cgccgccgaa gttcgtcttc
      421 ggcagccggg gggaaacgga ccgcgcaggc cgggcgctgg acagcatcgt ctgtccgggg
      481 acgatcgtct ccggcggcct cgtggagcgg tgcattctgg gcccgcgcgt gcggatcaac
      541 agctacgccc aggtttccga ttcaatcctg ttcgaggggg tcgatgtggg ccggcacgcc
      601 aaaatccagc gggccatcat cgacaaaggg gtcaaggtcc cgccagggat gcagatcggc
      661 ttcgacctgg ggctcgaccg caagcgcggc ctgacgatca gcgacggcgg catcgtcgtc
      721 atcgccaaag gggacggcct ggagcacatg ggcgatagcc cgcgaggcta gaacgtcgcc
      781 aatggacgaa acgaggaaag aagacgacgc ttcggcccag ggcttgccag gcgcgacgcg
      841 ttccagcacg tccgatcaag acccgcagtc catagccggc ttggagatca acctgggcga
      901 tttcacgcgc acgcaggttc tgatgaggtc cttgcgcctg acccttaaca atcagattgt
      961 tgtacaggtc gaggagatcg ccagcggtgt cagcgaagcg gggcgaaagt tcaagctggg
     1021 ggacgttgaa caggccaatc tggacgtggc ccgactgtat gccgccttcg atcaaaagac
     1081 caaccagtgg gagagtcagg cccgcaatct gcaacagcaa atgaaggcgc aggcggccaa
     1141 aaatcctcgg tccatttcga tcgacaaaat gaaccagatg aaggccgagc agacggccgt
     1201 ccgcacgcgc atccgcaccg cggaagtgca gttccgcagg ctgcatcaag ggctcgagca
     1261 ggcgtttacg attctgcaga gtcagccgcc accggcgact tccgccgcgc cagcggatct
     1321 ccctccgggc tttctggccc gcttcgaggc ggccgcgagc gcagaccggc cggcggttgt
     1381 gagagagtta ttcgagatcg aagccgagtt gaaggtcagc gtcacgcggg gcaagtccac
     1441 agggtatgaa atccggtttt cgcccacgcc agcgaagggc cgcctctatt ttctgacgac
     1501 cacggctcaa atcattcgcc tggagcagat cggcaattct gtcgttattt tggacctggt
     1561 gagcggcgaa gaaacggaga tgaagctgtc cgatttcgta aaacgagtgc aagccggcgc
     1621 gtcgttgctc caacctcggt agtccggtcc accacggttg aaatgcggcg cggccacgcg
     1681 tcgccaccct gccttcggcc gggtaaactg tggatttgaa cgccgtccgc caattgcatt
     1741 tggtcgtttt gctcgtcggt ggcgcgtaat cgttgatcgg acttgctact cagttgctta
     1801 ccgtacttcc gcagagcgaa cgagaaacga ttgctcgagc aatttgattt cgaggaccga
     1861 tgcaccgaac gccacggggc gcgagcaagt tccttcgtac cgaacggtgc catcctcttg
     1921 caatgtgggc ttgaggaaac cactcgccgt aataaggagt tcgccggttg cgccacggcg
     1981 caatatccgg aatactacag gaagaggcgg gcctcgatga ctggcgggac gtttcgcgtc
     2041 aacgcgaatg ctgaagtcgg aggcggggac cgtttggccg agcgcctggg gcgaacggtc
     2101 gtcgttggca cttgtgacgg gctgacccag atcgaacatg ggaagctcga tcaacggcag
     2161 gctgggatcg atcttggcct tcatggcatc ttcgtgcggc ggaggcttgt aggggtggcg
     2221 tccgcacccg tgtcccccga ccgcgagcat cacgccgacg acgatcgcga cgcaggtcca
     2281 catcagccgt tgggacgctg ccctcgccag gtctggtcga attcgcatta cttgacactt
     2341 acatttcggt gacgaaaaag tactgggtct tcacgacgcc tgggtcccag atttccggct
     2401 tgtagtgtcc cacaggatac gtcgagtccc agggtggctg gttattcgca tcgggtccat
     2461 cgactgtctt tgaccacatc gtttcctcga aaggatcggg tatggatcca ggtgcggcat
     2521 tgatggtcca tcctggtccg tattcgtccc attcctcgtc aacttccagg tactggagca
     2581 gcaaagcgtc ctcaccttcg ggacccatgc cggtgagatc gatggacttg attccataca
     2641 ttggatttcc agcgaatgta aatgaccatc ccgcgtgcaa ggcaatgggt gtcagcaaga
     2701 ccgtcgcggt cacaaagagc caggacagga tcgagcgtgt catctgtgtg cctccataac
     2761 gaggaaatga gaacgacaat cggctcaggg actgccggaa cgcgaggcga gccggcgcca
     2821 ctggaccaga tcaatagcct ggttgacgga ctggacgctt ccatccgcgt tggcaacatt
     2881 cacgaccgaa ccgtggaagc tggcactcga aaatgcggag gcgtcaatgc tcgcgccatt
     2941 gaggcaactg ggcgaattgg gccccaagac gtggttgtag agcgttcccg tgattcctcc
     3001 tcgaatccac ggacgtccgc accacaggtt gtccgtgaag ctgttcggcg cggaatcagg
     3061 aagattcgcg caggcctggg caaactggtc gagttgatct ttaccgccca gcgcaggcgc
     3121 cagccaatag acccgcagcc aatggtaaga accgtccgct gccaacactt cggagaacat
     3181 cgccgtgttg ctcaagccat cgctgatcga gctggaagtc agcgtgtcga ttccgccatg
     3241 cggatgcaag cgatagccaa agacaccgtt gtagccgtac cgctgcgatc ctgatccgca
     3301 gttgccaaaa tagcttgtag ccgcccagtc cctgatcaca acgcgcgacg aagcgtcgct
     3361 aggacgctgg aagattgcaa cccgcacgcc ctgcaagttc ccctggtaat ccgtttgatt
     3421 tatcgaaaag tcgaattggc gaaagagact ttgctgctcc aggtgaggaa gcagagcgac
     3481 atggaatgag taattcttgc gcatcgctcg cggcaaaacc ccgtgcagtt gctcgtactg
     3541 atgcacagcc agactgagtt gcctgagatt gctggcacat tcaagtctgc ggccagattc
     3601 gcgcacggcc tggaccgccg gcaggagcaa ggcgaacagg attccgatga tcgccaagac
     3661 gacgatc
//
LOCUS       DASUGB010001181         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_272939, whole genome shotgun sequence.
ACCESSION   DASUGB010001181 DASUGB010000000
VERSION     DASUGB010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272939"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFV87_22390"
     CDS             complement(<1..369)
                     /locus_tag="VFV87_22390"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX5106593.1"
                     /translation="MPARWASAAEGAESAEITPAVENNVSQGALRIVQKDGSIVECPL
                     KHTDVQADISGFIARVMVTQTFHNPTKEKIEAVYVFPLPHESAVDEMTMVLGERKIVG
                     LIKRRAEARSIYEQALASGQT"
     gene            717..1328
                     /gene="rpsD"
                     /locus_tag="VFV87_22395"
     CDS             717..1328
                     /gene="rpsD"
                     /locus_tag="VFV87_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5106594.1"
                     /translation="MARHHGPKARVNRRLSTLVYESAGATRALERRENPPGMHTRSRK
                     LSNWGIASAEKQKIKHYYGLGERQLRRYYSMVVAKKGDTGAQMLALCERRLDNVVRRS
                     GLTKTRPQARQGIVHGHFYVNGIKVTSPSYQLRPGDVITVKPKEPIYNLYKSVVGDGD
                     SQQVPDWITMDPQSLRVTMVGVPGDGDVSLPVNVNVVVEFLAR"
     gene            1464..>2225
                     /locus_tag="VFV87_22400"
     CDS             1464..>2225
                     /locus_tag="VFV87_22400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5106595.1"
                     /translation="MHSQLVVLGGGPGGYAAAFLAADEGLEVTLVEADPRLGGTCLLR
                     GCIPSKALLHVARVIGEVDELRHDWGVEFPQPKIDIAKVRERKEKVITTLSGGLKQLA
                     KRRNVKVIHARGTFENSTTLKLEGDDASIPENGKLTFDHCILATGSVPAMPPPWQLGS
                     DRVMDSTGALNLADLPKSLLVIGGGYIGLEMGTVYAQLGSQVSVVELTETLLPGADRD
                     LVKPLHKRVSALFGERIYLNTKVGSLGLRDGKVEVA"
BASE COUNT          415 a          736 c          693 g          381 t
ORIGIN      
        1 ggtctgaccg gatgccaggg cctgttcata gatcgagcgg gcctcagccc gccgcttgat
       61 caggcctacg atcttccgct cgcccaggac catcgtcatt tcatccacgg ccgattcgtg
      121 cggcagcggg aagacataca ccgcctcgat tttctccttc gtcgggttgt ggaaggtctg
      181 cgtcaccatc acgcgggcga tgaagccgct gatgtccgcc tgcacgtcgg tgtgcttcag
      241 cgggcattcg acgatgctgc cgtccttctg tacgatccgc agcgctccct gcgagacgtt
      301 gttctccacg gcgggcgtga tctcagccga ttctgcccct tctgccgcac tcgcccatct
      361 tgcgggcatt gccgacacga tgccgagaac agccagaaac ccgatcaacc gagtgggaga
      421 acctgcggca aacatggcac gcacctttcg cgggagtaga gcgggaaccg cggaccagcg
      481 gcgaacgccg gggaaccgaa ttaagacgac gctacgtccc gaactgttct ctggtacggc
      541 attgtaaaac ccaggaaacg gtaaacggag gtcgaggaag cacaatgcct tcgtccggca
      601 tggcgtaaac gcgtaagccc aagacgtcga tttcgattga agacgcccct tgttgccttg
      661 cccggaatcg gccatgataa ggggctttcc tgtaagaggt tacggagttt ccgctgatgg
      721 ctcgtcacca cggtcccaag gcccgcgtca accgccggtt atcgacgctc gtctatgaaa
      781 gcgccggcgc cacccgggcc ctggaacgcc gcgaaaaccc ccccggcatg cacacccgca
      841 gccgcaagct ctccaactgg ggcatcgcct cggccgagaa gcagaagatc aagcattact
      901 atggcctggg cgagcggcaa ctgcggcgct attacagcat ggtcgtggcg aagaagggcg
      961 ataccggcgc ccagatgctc gccctctgcg agcggcggct ggacaacgtc gttcgccgct
     1021 ccggtctgac gaagacccgc ccgcaggccc ggcagggaat tgtccacggc cacttctacg
     1081 tcaacggcat taaggtcacc agccccagct accagcttcg ccccggcgac gtgatcaccg
     1141 tcaagcccaa ggagccgatc tacaacctct ataagagcgt ggtcggcgac ggcgacagcc
     1201 agcaggtgcc cgactggatc acgatggacc cccagtcgct gcgggtcacg atggtcggcg
     1261 ttcccggcga cggcgacgtc agcctcccgg tcaacgtcaa cgtcgtggtc gagttcctcg
     1321 cacggtaacg tggggcaggc ttccagcctg ccgctcgtgg cgtaggcttc cagcctgcca
     1381 agagttcagg cggcgcttcg caaggagcgt cgtttggcgt ttcttgactt tgggaaccgc
     1441 gaccactggt cgcggctatg accatgcact cacaactcgt tgtcctcggc ggcggtcccg
     1501 gcggttatgc ggcggcgttc ctcgcggccg atgaggggct ggaggtcacg ctggtcgaag
     1561 ccgacccgcg gctgggcgga acgtgcctgc tgcgcggctg cattccgtcg aaggccctct
     1621 tgcacgtggc ccgcgtcatc ggcgaagtcg atgagctgcg gcacgactgg ggcgtcgaat
     1681 tcccgcagcc aaagatcgac atcgccaaag tccgcgagcg gaaagagaag gtcatcacga
     1741 cgctttccgg cgggctcaag cagctcgcca agcgccgcaa cgtcaaggtg atccacgccc
     1801 gcggcacgtt cgaaaacagc accacgctca agctggaagg ggacgacgcc tcgatccccg
     1861 agaatggcaa gctgacgttc gaccattgca tcctggccac cggcagcgtc cctgccatgc
     1921 cgcccccgtg gcagcttggc agcgatcgcg tgatggattc gaccggggcc ctcaacctgg
     1981 ccgatctgcc gaagagcctg ctcgtcatcg gcggcggcta catcggcctg gagatgggga
     2041 ccgtctatgc ccagctcggc tcgcaagtca gcgtcgtcga gctgaccgaa acgctgctgc
     2101 cgggggccga tcgcgacctg gtcaagccgc tgcacaagcg ggtcagtgcc ctgttcggcg
     2161 agcggatcta cctcaatacc aaggtcggct cgctcggcct gcgcgacggc aaggtcgagg
     2221 tcgcc
//
LOCUS       DASUGB010001182         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1800614, whole genome shotgun sequence.
ACCESSION   DASUGB010001182 DASUGB010000000
VERSION     DASUGB010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800614"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFV87_22405"
     CDS             <1..704
                     /locus_tag="VFV87_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106596.1"
                     /translation="PNFAFETCEVVVVVGQDGLVANTAKYVRGLPIVAINPDPARIDG
                     VLLPFQPKQARTIVRRVLDGKHKQRSVTLAEAELNDGQKLLAFNDFFLGAATHVSARY
                     VLEAGGHSEPQSSSGVLVSTGAGSTGWLSSVFNMAGGIARFLGEDVEDRPQLRWEDRK
                     LVWAVREPFVSKRSQASLVAGWLQEGQELIVESLMPEGGVIFSDGIEKDYLPFTSGTI
                     ARVRVSSQRANLVVG"
     gene            complement(729..1043)
                     /locus_tag="VFV87_22410"
     CDS             complement(729..1043)
                     /locus_tag="VFV87_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5106597.1"
                     /translation="MTPVPINLALTDDSRLLIEWSDGARRKYGFRALRDACPCATCRE
                     KQSAPKDPLVLPTLGAGELAPLKVTGMTPVGNYAYSIAFSDGHNTGIYPLTLLRELGE
                     PA"
     gene            complement(1220..2473)
                     /locus_tag="VFV87_22415"
     CDS             complement(1220..2473)
                     /locus_tag="VFV87_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX5106598.1"
                     /translation="MRFHPPIVEYCRRQFPALSRRVDGHAAAYFDGPGGTQVPQRVID
                     AVSHYLAHTNANHGGLFATSQESDRVLEDAHQALADLLGADDPQSVIFGQNMTSLTFA
                     LSRALARTWKKGDEIVVTRLDHDANVAPWVLAARDAGAEIRWVGFREDDCTLDLDQLQ
                     HTINERTRLVAVGAASNATGGINPIRDIASLAHAVGAHVFCDAVHYAPHVLIDVQALG
                     CDFLACSAYKFFGPHVGLMWGQRHLLESLEAYKVRPAPTALPDKWMIGTQSHESIAGA
                     AAAVEYLADIGRRITGNESLPRRGALREAYREIGLYERHLAGRLLAALEKMPDIKVWG
                     ITSLQRLEERVATIAFTHERFTAHEIAQQLGRQGFFTWHGNYYALNLTETLGLEPHGM
                     LRVGLLHYNTESEVDRLLEALHGLR"
     gene            2540..2752
                     /locus_tag="VFV87_22420"
     CDS             2540..2752
                     /locus_tag="VFV87_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106599.1"
                     /translation="MNEEELKLAIFTPPFEPKRIRLTNGETYEIRHPGAIAIGHRACS
                     VVVNGRIHMVSNLHIAQVEPIDAAVH"
     gene            complement(2789..>3973)
                     /locus_tag="VFV87_22425"
     CDS             complement(2789..>3973)
                     /locus_tag="VFV87_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5106600.1"
                     /translation="KHNRFYAEKLASVNLPLESLDQLPDLPFTFKEELSTAKHDGEFA
                     ANLTYPLERYVRYHQTSGTRGRPLSVLDTADDWRWWIECWQFVLDAAEVGPEDRALLA
                     FSFGPFIGFWSAHDAAVARGTLVIPSGGLSTLSRVEMVRRTQATILFCTPSYALRLAE
                     VAHENHIDPASLEVRRIIVAGEPGGSVSALRRRIETTWNARLTDHAGASEVGPWGYGD
                     KEGRGLFVLESEFIAEFRSLATGQRAADGEEAELVLTSLGRLGSPVIRYRTGDIVRPR
                     WGLVEDNGFVLLEGGVLGRADDMLIVRGVNVFPSAVEQILRSFPEIVEYRVTVTRQGS
                     LDALIVEIEDRLDDPARVARELKLRLGLKVDVRCAKLGSLPRFEGKGKRFHDQRQTNY
                     QI"
BASE COUNT          693 a         1240 c         1335 g          705 t
ORIGIN      
        1 tgccgaactt cgcgtttgaa acgtgcgagg tggtcgtcgt cgtcgggcag gatggcctgg
       61 tcgccaacac ggccaagtac gttcgcggcc tgccgatcgt cgcgatcaat cccgaccccg
      121 cgcggatcga cggtgtgctg ctcccctttc agccgaagca agcccgcacg attgtccgtc
      181 gcgtgctgga cggcaagcac aagcagcggt cggtgacgct cgcggaagcc gagctgaacg
      241 acggacagaa gctgctggcg tttaacgatt tcttcctcgg cgcggccacc cacgtctcgg
      301 cccgctacgt cctcgaagcc ggcggccaca gcgaaccgca atcgagcagc ggcgtgctgg
      361 tttccaccgg cgcgggctcg accggctggc tgtcgagcgt gttcaacatg gccggcggga
      421 tcgcccggtt tctgggcgaa gacgtcgaag accggccgca gctcaggtgg gaagaccgca
      481 aactcgtctg ggccgtccgc gagccgttcg tcagcaagcg gtcgcaggca tcgctggtcg
      541 ccggctggct gcaggaaggc caggagctga ttgtcgagtc gctgatgccc gaagggggcg
      601 tgatcttttc ggacgggatc gagaaggact atctgccgtt caccagcggg acgatcgccc
      661 gcgtccgcgt cagttcgcag cgtgcgaatc tggttgtcgg ctgacttggc ggcgaacggc
      721 aggctccgtc acgccggctc gcccagctcg cgcagcagcg taaggggata gatgcccgtg
      781 ttgtgcccgt cgctgaaggc gatgctgtag gcgtagttgc cgacgggcgt catgccggtt
      841 accttcagcg gcgcaagctc tccggcccca agtgttggca acaccagggg atctttcggc
      901 gcggattgct tctcgcggca ggtcgcacac gggcaggcgt cgcgcagtgc ccgaaagccg
      961 tacttcctcc gtgccccatc gctccattcg atgagcagac ggctgtcgtc ggtgagggca
     1021 agattgatgg ggaccggtgt catcgaatca aatccagctc ttgctctttc tcctgctcgt
     1081 aatcctattc ctaattctaa tctttctctt gctcttgctc gctcttgctg ccccgcgcgg
     1141 ccgcggcggg cggaaggagc aggagaaaga gcaggattaa gattaggaag aggattaaga
     1201 gcaagagcaa gagaaggatt tatctcagtc cgtgcaaagc ctccaagagc cgatcgactt
     1261 cgctctcggt attgtagtgc agcaggccga cgcggagcat gccgtgcggc tccaggccga
     1321 gagtctcggt gagattgagg gcgtagtaat tcccgtgcca ggtgaagaag ccctggcgtc
     1381 cgagctgctg ggcgatctcg tgggccgtga agcgctcgtg ggtgaaggcg atcgtcgcga
     1441 cgcgctcctc caggcgctgc agggatgtga tgccccagac cttgatgtcg ggcatttttt
     1501 ccagcgccgc cagcagccgg ccggcgagat gccgctcgta gaggccaatc tcgcgatacg
     1561 cttcgcggag cgcgccgcgc cgcggcagcg actcgttgcc ggtgatccgg cggccgatgt
     1621 cggccaggta ctccacggct gccgctgcgc cggcgattga ctcgtggctt tgcgtgccga
     1681 tcatccactt gtccggcaga gcagttggag ccggccgcac cttgtacgct tccaggctct
     1741 ccagcaggtg ccgctgcccc cacatcaggc cgacgtgcgg gccaaagaac ttgtaggccg
     1801 agcacgccag aaaatcgcag cccagggcct gcacgtcgat cagcacgtgc ggggcgtagt
     1861 gcaccgcgtc gcagaacacg tgcgccccga cggcatgggc caggctggcg atgtcgcgaa
     1921 tcggattgat gccgccggtc gcgttcgacg cggcccccac cgcaaccagc cgcgtgcgtt
     1981 cgttgatcgt atgctggagc tgatcaagat cgagcgtgca atcgtcctcg cgaaagccga
     2041 cccagcggat ctcggctccg gcatcgcggg cggcgagcac ccacggcgcg acgttggcgt
     2101 cgtgatccag ccgcgtaaca acgatttcgt cccctttctt ccacgtacgg gccagcgccc
     2161 gcgagagggc gaacgtgagg gatgtcatgt tctggccgaa gattacggac tgcgggtcgt
     2221 cggcgcctaa aagatcggcc agggcctgat gggcgtcctc cagcacgcgg tcgctctcct
     2281 gcgacgtggc gaacaggccg ccgtgattgg cgttggtatg ggccaggtaa tggctgacgg
     2341 catcgatcac ccgctgggga acctgcgtcc cgccggggcc gtcgaaatag gcggctgcat
     2401 gtccatccac gcgccgcgaa agagcgggaa actggcggcg gcagtattca acgatgggag
     2461 ggtggaatcg catgctcaag gctataatag gagttgaggt tttgtcacac agcggcagac
     2521 cagcaccgag ggaatcggca tgaatgagga agagctaaag ctggcgattt tcacgccgcc
     2581 tttcgaaccc aagcgcatcc gcctgacgaa tggagagacc tacgaaatcc gccatccggg
     2641 ggcgattgcg atcggtcatc gcgcttgcag cgtggttgtt aacggacgaa tccacatggt
     2701 gtcgaacctt catattgcac aagtcgaacc catcgacgcc gcggtccact agccgtcgtt
     2761 tgcgttgcgg caggagaacc gcgcgccgct aaatctggta attcgtctgc cgctggtcgt
     2821 ggaagcgttt gcccttgcct tcaaaccgcg ggagcgagcc cagcttggcg cagcggacat
     2881 ccactttcag tccgaggcgg agcttgagct cgcgggcgac gcgggccgga tcgtcgagcc
     2941 ggtcttcgat ctccacgatc aaggcgtcca ggctcccctg ccgggtcacg gtgacgcggt
     3001 attcgacgat ctcgggaaac gatcgcagga tctgctcgac ggcgctcgga aagacgttga
     3061 cgccccggac gatcagcatg tcgtcggccc ggcccaggac gcccccttcc agcagcacga
     3121 agccgttatc ttccaccagg ccccaccggg ggcggacaat gtcgccggtg cggtagcgga
     3181 tgaccgggct gccgaggcgg ccgagcgacg tgagcaccag ctccgcttcc tcgccgtcgg
     3241 cagcccgctg gccggtggcc agcgagcgga attcggcgat gaattcgctt tccagaacga
     3301 ataggccgcg tccctctttg tcaccatagc cccacgggcc gacttccgag gccccggcgt
     3361 ggtcggtcag gcgggcgttc catgttgttt cgatccgccg tcgcagggcg gagaccgagc
     3421 cgccgggctc gccggcgacg atgatgcgcc gcacctcgag gctcgccggg tcgatgtggt
     3481 tttcgtgggc gacttcggcc aggcggagag cgtagctggg cgtgcagaag aggatggtgg
     3541 cctgtgtccg tcgcaccatc tcgacccgcg agagcgtgct caggccgccg ctgggaatga
     3601 ccagcgtgcc ccgggcgacc gccgcgtcgt gagcgctcca gaagccgatg aatgggccaa
     3661 acgagaaggc caaaagcgcc cggtcttccg ggccgacttc cgcggcgtcg agcacaaact
     3721 gccagcattc gatccaccac cgccagtcgt cggccgtgtc gagcaccgac agcggtcggc
     3781 cgcgggtgcc cgacgtttgg tgatagcgga cgtaccgctc gagcggatag gtcaggttgg
     3841 cggcgaactc gccgtcgtgc ttggccgtgg agagctcctc cttgaaggta aacggcaggt
     3901 cgggcagttg atcgagcgac tcgagcggca gattgaccga cgcgagcttc tccgcataga
     3961 agcggttgtg ctt
//
LOCUS       DASUGB010001183         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_967176, whole genome shotgun sequence.
ACCESSION   DASUGB010001183 DASUGB010000000
VERSION     DASUGB010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967176"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VFV87_22430"
     CDS             <1..137
                     /locus_tag="VFV87_22430"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5106601.1"
                     /translation="RGQRPNEPARVLYTAECLAQVRGVSLAELAGQTTANARTLLRLV
                     "
     gene            196..1251
                     /locus_tag="VFV87_22435"
     CDS             196..1251
                     /locus_tag="VFV87_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5106602.1"
                     /translation="MTEHVVIIGSGPAGWAAAIYAARANLRPLVFEGAETEENRVAGT
                     LPLGQLNLTTEVENYPGFPAGHLHHFLRSALPKERLDTFLEPLHEETHAVTGPELMNL
                     MRQQAINFGARVITDDVVRVDFQRQPFTLATLGGDTHETLTAIIATGARANYLGLPSE
                     ERFKNRGVSACAVCDGALPRFRDKPLVVVGGGDSAIEEATYLTKYASTVHMLLRRDVF
                     RASKIMQERATSHPKIKLHFHREVDEVLGDDKQGVTAIRMKNNLEGGTEDLSAGGLFL
                     AIGHTPNTAFLKGQVELNAMGYIKYTTLMRTLTSVPGVFAAGDVADDYYRQAITSAGT
                     GCMAALDAERYLAAREH"
     gene            1273..2196
                     /locus_tag="VFV87_22440"
     CDS             1273..2196
                     /locus_tag="VFV87_22440"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5106603.1"
                     /translation="MPELPDILGYLTALRPRIVGQPLERLVVRSPLLVRTVEPDLFSL
                     EGQSVQDLSRLGKRIVWHFAGETFAVFHLMIAGRFQWKQQAARPGGKNDLAAFVFPGG
                     TLMLTEASQKKRASLHVVRGRAALAAHDRGGLEPLDCSAEAFRGAILRENHTLKRALA
                     DPRLFSGIGNAYSDEILHAARLSPVTLTQRLSDEETSRLYAAVQAVLGQWTARLVGEA
                     QQRFPEKVIAFRPGMAVHGRYRQPCPVCGTPVHRIVYAENETNYCPRCQTGGKLLADR
                     SLSRLLKGDWPRTIDEWEEQRGTGKRVEGRG"
BASE COUNT          430 a          832 c          801 g          431 t
ORIGIN      
        1 ttcgcggcca gcgccccaac gagccggcgc gagtcctgta caccgccgag tgcctggccc
       61 aagtccgcgg agtgtccctc gcggaactgg ccgggcaaac gacggccaat gccaggactt
      121 tgctgcggct cgtataattg cctcgttttc accggcatca acgcgactgc cttgatttgt
      181 aggggaatat gcatcatgac cgagcacgtc gtcatcatcg gcagcggacc tgccggctgg
      241 gccgcagcca tctatgccgc ccgcgcgaac ctgcggccgc tggtgttcga gggggccgag
      301 accgaggaga accgcgtggc cggcaccctc ccgctggggc aactcaatct gaccaccgag
      361 gtcgaaaact accccggctt tcccgcgggt cacctgcacc atttcctccg aagcgccctg
      421 cccaaggagc ggctggacac gttcctcgag ccgctgcacg aagagacgca cgccgtcacc
      481 ggtccggaac tgatgaacct gatgcggcag caagcgatca atttcggcgc ccgcgtgatc
      541 accgacgacg tggtgcgcgt cgatttccag cggcagcctt ttaccttggc gactctgggg
      601 ggcgacaccc atgagacgct gaccgcgatc atcgccaccg gcgcgcgggc gaactacctg
      661 ggcctcccct cggaagaacg gttcaagaat cgcggggtca gcgcctgcgc cgtgtgcgac
      721 ggggccttgc cgcggttccg cgataagccg ctggtcgtgg tcggcggcgg cgattcggcg
      781 atcgaggagg cgacgtacct caccaagtac gcctctacgg tccacatgct cttgcggcgc
      841 gatgtcttcc gggccagcaa gatcatgcag gagcgggcca cctcccaccc gaagatcaaa
      901 ctgcatttcc accgcgaggt ggatgaagtg ctcggcgacg acaagcaggg ggtgaccgcc
      961 attcgtatga agaacaatct ggaagggggc acggaagacc tgtcggccgg cggcttgttc
     1021 ctggctattg gccacacgcc caacaccgcg ttcctcaagg ggcaggtcga gctcaacgcc
     1081 atgggttaca tcaagtacac gaccctgatg cgcacgttga ccagcgtccc tggcgtcttt
     1141 gccgccggcg acgtggccga cgactattac cgccaggcca tcacctcggc gggcaccggc
     1201 tgcatggccg cgctcgatgc cgagcgctac ctggcggcgc gcgaacatta gccgcagccc
     1261 gagagggccg cgatgcccga gcttcccgac atcctgggct acctgaccgc cctgcggcca
     1321 cggatcgtcg gccagccctt ggagcggctc gtcgttcgca gcccgctgct ggtccgtacc
     1381 gtcgagccgg acttgttttc gctcgaggga cagtcggtac aggacctgtc gcggctgggg
     1441 aagcggatcg tctggcattt cgccggcgag acgttcgccg tcttccacct gatgattgcg
     1501 gggcgattcc aatggaaaca acaagcagcg cggccgggcg gcaagaacga tctggcggca
     1561 ttcgtctttc cgggcggcac gctcatgctg accgaagcca gccagaagaa gcgggcgtcg
     1621 ctgcacgtcg ttcgcggccg cgccgctttg gccgcgcatg accggggcgg cctcgagccg
     1681 ctggactgct cagccgaagc gtttcggggg gcaattctgc gcgaaaacca cacgctcaag
     1741 cgagccctgg ccgatccgcg gctcttctcg ggaatcggca acgcctattc cgacgagatc
     1801 ctgcacgccg cccggctttc ccccgtcacg ttgacgcagc gactcagcga cgaagaaacc
     1861 agccgactct atgcggccgt gcaagcggtg ctcggccagt ggacggcgcg gctggtcggc
     1921 gaggcccagc agcgatttcc ggagaaggtg atcgccttcc ggccgggaat ggcggtccac
     1981 ggccggtacc gccagccctg ccccgtgtgc ggcacgccgg tgcaccgaat cgtttatgct
     2041 gagaatgaaa cgaactattg cccccgctgc caaaccggcg gcaagctgct ggccgaccgc
     2101 tcgctgtcgc ggctgctcaa gggcgattgg ccgcgcacga ttgacgagtg ggaggagcag
     2161 cggggtactg ggaagagggt agagggtaga gggtagcagg agccagtggt cagtggtcgg
     2221 gcgtccaaat ttgccattac tcccttcgcc tccctgtatg acctccgacc tttggccttt
     2281 gtccactgac tcctaccctc taccctctaa aattccgccc gcttgctctt cccccagccg
     2341 tgggatcggc cataatggag gcattaccaa ggttcccggc gcttggccgg gcgggttgac
     2401 taaggttcgc agcgattgcg ctgccatgtc ttggcccgca tcgtcctggc ccgcacttgc
     2461 tgtcgcgtgc tgcgtcggca gtcgcgaaga acgt
//
LOCUS       DASUGB010001184         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1938659, whole genome shotgun sequence.
ACCESSION   DASUGB010001184 DASUGB010000000
VERSION     DASUGB010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938659"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFV87_22445"
     CDS             <1..206
                     /locus_tag="VFV87_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEX5106604.1"
                     /translation="DGPTDAAGAILDGPLLARAAELQLDPRDYLRRNDAYRFFEPLGG
                     LIRTGPTDPNVCDLRVVVVDRST"
     gene            complement(214..1512)
                     /locus_tag="VFV87_22450"
     CDS             complement(214..1512)
                     /locus_tag="VFV87_22450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5106605.1"
                     /translation="MNRPDTPTDTARWRQLALLAAVELLAMALWFSASAVTPALKQPW
                     ELSDGAAAWLTISVQLGFVIGALASAVLNLSERIAPPRMLAGCALLGAIFNGLIPLGI
                     SDELGRTAGGFAMVVLLRMATGVMLAGVYPTGMKLMATWFKQRRGLAIGVLVGALTVG
                     SASPHLVNALPLDEWSRQYLGGVSGWAVVMLAASASAVVAAALAAMFVRSGPHLPAAA
                     RFDWRYFARVWAEPALRRANFGYLGHMFELYAMWTWAPQLLAASYAHADWNASAARAA
                     GFATVAIGSVGCVLAGLAADRAGRCWTTVVSLVLSGGCALVAGALFDWPGALTVVCLV
                     WGFAVVADSAQFSAAISELCDPQFVGTALTIQTCAGFLLTTLTIQAVPWMLGETGTSG
                     GSAAWWPALAILAAGPVFGVFHMLRLRQMPEAEKMAGGAR"
     gene            1647..2351
                     /locus_tag="VFV87_22455"
     CDS             1647..2351
                     /locus_tag="VFV87_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106606.1"
                     /translation="MNDRTRALEILREAKAVLADRLTEKVLEQADEILADARGDSYMN
                     EIESLYEQVGLKLTHVSQMLSNLPAPQPEPHSTTAAAQHAPEDAFQMATDPPGDDVFV
                     AETLPALAGPLYIATPALPAPKTAESAKQRATTSALQAFAAQIQAGDLLAAGRTLGGL
                     FDLEDSRAIACAATFAQRVRTEAGFFRKVMELRTEVHSNNPQRALLLLLDCFGLSRGE
                     SAEVLQTIQRRRLRHG"
     gene            2363..2569
                     /locus_tag="VFV87_22460"
     CDS             2363..2569
                     /locus_tag="VFV87_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106607.1"
                     /translation="MSRKPFVFQETEPMDGKAKKRLEVIRQKLLVLKQRLAGAKRQDD
                     EPGEVQRLEQEIAQLEAEATKLRS"
BASE COUNT          533 a          966 c          924 g          409 t
ORIGIN      
        1 aagacggccc caccgacgcc gccggtgcga tcttggacgg cccgctcctg gcccgcgccg
       61 ccgagctcca gctcgacccg cgcgactacc tccgccgcaa cgacgcctac cggttcttcg
      121 agcccctcgg cggcctgatc agaaccggcc ccaccgaccc caacgtctgc gatttgcgcg
      181 tcgtcgtggt ggaccgcagc acttagattc agctcacctt gcgccgccgg ccatcttctc
      241 cgcctccggc atctgccgca ggcggagcat gtgaaacacg ccgaagaccg ggccggcggc
      301 gagaatcgcg agggcgggcc accaggcggc acttccgccg cttgtgcccg tttcgcccag
      361 catccacggc acggcttgga tcgtgagcgt cgtgagcagg aagccggcgc aggtctgaat
      421 cgtgagagcc gtgccgacga attgcgggtc gcacagctcc gagatcgcgg cgctgaattg
      481 ggcgctgtcg gcgacgaccg cgaagcccca gacgaggcag acgacggtta aagcgcccgg
      541 ccaatcgaag agcgcccccg caactagcgc gcagccgcca ctcagcacca ggctgacgac
      601 cgtcgtccag cagcggccgg cgcggtcggc agccagcccc gcgagcacgc agccaacgct
      661 gccaatggcg accgtcgcga agccggcggc ccgcgcggcg gatgcgttcc aatcggcgtg
      721 cgcgtagctg gccgccaaaa gctgcggcgc ccacgtccac atggcataca gctcgaacat
      781 gtggccaagg tagccgaagt tggcccgccg caacgccggc tcggcccaca cgcgggcgaa
      841 atagcgccag tcgaaccgcg ccgcggccgg caggtgcggc ccgctgcgga caaacatcgc
      901 cgccagagcc gccgcgacca ccgcgctggc cgaagccgcg agcatcacca ccgcccagcc
      961 gctgacaccg cccaagtatt gccgcgacca ctcatccagc ggcagcgcgt tgaccagatg
     1021 cggcgaggcc gatcccacgg tcagcgcgcc aacgagcacg ccgatcgcca gcccgcgccg
     1081 ttgcttgaac caggtggcca tcagcttcat gccggtcgga tagacgccgg ccagcattac
     1141 gcccgtcgcc attcgcagca gcaccaccat cgcaaagccg ccggcagttc gccccagctc
     1201 atcgctgatg cccagcggga tcaggccgtt gaagatcgcc cccaagagag cgcagccggc
     1261 cagcatgcgc ggcggggcga tccgctccga gaggttgagc acggcgctcg ccagggctcc
     1321 gatgacgaac ccgagctgga cgctgatcgt cagccaggcg gccgcgccgt ccgaaagctc
     1381 ccagggctgc ttgagggcgg gcgtcacggc ggaagccgag aaccagaggg ccatcgccaa
     1441 cagctcgacc gccgccagca gcgcaagctg acgccagcgg gcggtgtcgg tgggcgtgtc
     1501 tggcctgttc aaggtgctgg cgtcgatagc tcaggttccc gggcgaatct ctgcggcggc
     1561 ggaatccgca aacccgatcc tttccacatc cgccgctgcg aaataagctt agtaaggagc
     1621 cagttgcttc gggagtctgg atcaacatga acgaccgcac gcgtgccctg gaaatccttc
     1681 gcgaggccaa agcggtgctc gccgaccgcc tcacggagaa ggtactggag caggccgacg
     1741 agattctggc cgatgcccgc ggcgattcgt acatgaacga gatcgaatcg ctctacgagc
     1801 aggttgggct caagctgacg cacgtcagcc agatgctctc gaacctgccg gccccgcagc
     1861 cggagccgca ctcgacgacc gccgcggccc agcacgcgcc ggaagacgcc tttcagatgg
     1921 cgaccgaccc gccgggcgac gatgtcttcg tggcggagac gctgccggcg ttggccggtc
     1981 cgctgtacat tgccaccccg gcgcttcccg cgcccaagac ggccgagtcc gcgaagcagc
     2041 gggcaacgac ttctgctttg caggcgttcg ccgcccagat tcaggcgggc gacctgctcg
     2101 cagcggggcg aacgctgggc gggttgttcg acctggagga ttcgcgggcg attgcttgtg
     2161 ctgcgacgtt cgcccagcgc gtccgcaccg aagcgggctt tttccggaag gtgatggaat
     2221 tgcgaactga ggtccatagc aacaatccgc agcgggcctt gcttctactc ttggattgtt
     2281 tcggcctgtc gcgcggtgaa tcagccgaag tcctccagac gatccagcgg cggcgattgc
     2341 ggcacggata actcgtctag agatgagtcg aaagccattc gtctttcaag agaccgaacc
     2401 aatggacggc aaagcgaaaa agcggctcga agttatccgg caaaagctgc tggtgctcaa
     2461 gcagcgcctg gccggagcga agcggcagga tgacgaacct ggggaagttc aaaggctcga
     2521 gcaggagatc gcccaactcg aggcggaggc gacaaagctg cggtcctaac aggcagccgc
     2581 gaattccacc ggacaaacag aacacactcg ctcttcgcac gattctgggc gtggggtaca
     2641 cttgcaggga aatctgcctt ctttggagca agtagccatg cacctgggaa acggggcgat
     2701 cacgcctgaa tgtgccgcaa tcacgatggg cgcggctgcg gctgggctgg ggttcgccgc
     2761 gtatgcgggt cgccgcgaaa tcgtggaccc gaacaagctg ttgatcgcgg caggtttggg
     2821 ttcgctggtc tt
//
LOCUS       DASUGB010001185         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_551281, whole genome shotgun sequence.
ACCESSION   DASUGB010001185 DASUGB010000000
VERSION     DASUGB010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551281"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFV87_22465"
     CDS             <1..519
                     /locus_tag="VFV87_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106608.1"
                     /translation="PLVLVLYLWLTGGLTLENFLFLAIGLVVMDVFVIMLGVHCGLNY
                     ANSRTAVGVSLGSVFFLFLGVVTLILMMISFSGSFQTQLAPFLAFVAGGSLGLFVTLG
                     IRNPSAAIGLASLLLPFATFFAVTNFLVDHLLEAFLVVAFTYGFTTAAMLVPALYEFD
                     IAMGRTKGAGEE"
     gene            complement(550..777)
                     /locus_tag="VFV87_22470"
     CDS             complement(550..777)
                     /locus_tag="VFV87_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106609.1"
                     /translation="MIQPLHQALLQRLAAVCELSPDIRFGQLVDFVGLLAAGESQRNL
                     PDIEDDELLAALEEHHKNLLDRQSMSAGRSA"
     gene            complement(774..1073)
                     /locus_tag="VFV87_22475"
     CDS             complement(774..1073)
                     /locus_tag="VFV87_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106610.1"
                     /translation="MATYFRDVDPRELRAPTSRPTGADPLKLARQIAKCGSSTTGMPP
                     LVVYESLDGFLVVYDGVTRATRAAKLVPGVPIRIEVVGTIPRRYANEPKIGDLLP"
     gene            1176..1931
                     /locus_tag="VFV87_22480"
     CDS             1176..1931
                     /locus_tag="VFV87_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5106611.1"
                     /translation="MTAALQPAAEPLADVPHPRRPRHVAIIMDGNGRWAQRRHLPRIE
                     GHRRGVASVRRTVEEAARLHLEQLTLYCLSSENWKRPQREIDFLMHLLEQYMIEERST
                     ILRQNITVRVIGRREGIPAATLAEMDRTVEMSAANTGTRLCLAINYGGRSEMVDAVKQ
                     IAAEAKSGQLEPASIDEAAISERLYTAGMPDPDLLIRTAGEMRISNFLLWQISYAELW
                     VTDRCWPEFEIADFHQAIRDFASRDRKFGGIRP"
     gene            2031..>2452
                     /locus_tag="VFV87_22485"
     CDS             2031..>2452
                     /locus_tag="VFV87_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106612.1"
                     /translation="MLRWRLISAAVIVSVLLTLVWLDFRRAVFGVAGVWLFPVLLAVT
                     WMATEEVLGLLRAKGHRPVAWILYLGNGLITLAACLPMLFEFFDRPFPDDNPLGRFGW
                     PLVALAIATVGVFAAEMRRFEQPGQTTVLAALGIFTLV"
BASE COUNT          411 a          789 c          751 g          501 t
ORIGIN      
        1 ccgctcgtct tggtgctgta tctatggctg acgggcggcc tgacgctgga gaattttctc
       61 ttccttgcga tcggcctcgt ggtgatggac gtgtttgtca tcatgctcgg tgtccactgc
      121 gggctgaact atgccaactc gcggaccgcc gtcggcgtga gcctggggag cgtcttcttc
      181 ctgttcctgg gcgtcgtcac gctgatcctg atgatgatca gcttcagcgg cagctttcag
      241 acgcagctcg cgccgttcct ggcgttcgtc gccggcggca gcctgggctt gtttgtgacg
      301 ctcggcatcc gcaatccgtc cgccgccatc ggcctggcct cgctcctcct gccgttcgcc
      361 acgttcttcg ccgtcaccaa cttcctggtc gatcacctgc tggaagcgtt cctcgtcgtc
      421 gccttcacct acggcttcac caccgccgcc atgctcgtcc ctgccctgta cgaattcgac
      481 attgccatgg gccgcacgaa gggggcgggg gaagagtgag tccggccgtg cgcggtcaat
      541 cgcacctgtt caggcgcttc gtccggcgct catcgactgc cgatcgagca gattcttgtg
      601 gtgttcctcg agtgcggcca gcaattcgtc gtcttcaatg tccggcaaat tgcgttgtga
      661 ttcaccagcg gccaataggc ctacgaaatc gacaagctga ccgaagcgga tgtcgggtga
      721 caactcacat acggcggcca atcgctggag caacgcctgg tgcaatggtt ggatcatggc
      781 aacaaatctc cgatcttcgg ctcgttggca tagcgacgag ggatcgttcc gacgacctca
      841 atgcgaatgg ggacgccagg caccagtttg gccgcccgcg tggccctcgt cactccatca
      901 tatacgacca gaaacccgtc aagcgactca taaaccacca gcggcggcat tcccgtcgtc
      961 gagcttccgc atttggcaat ttgtcgggca agtttgagcg gatcggcgcc ggtcggtcga
     1021 gacgtcggcg cgcgcagctc ccgcgggtcc acgtcccgga agtaggttgc catcgcgaga
     1081 gtctatccga tcggcgccgt tgccgcccgc gcggggggca gggtacaacg taacaagagt
     1141 cgctgtgaat tggcctttgt gcgagctgaa ctgccttgac tgccgccttg caacccgctg
     1201 ccgagccatt agcggatgtg ccgcacccgc gccggccgcg gcatgtggcg atcatcatgg
     1261 acggcaacgg gcggtgggcc cagcggcggc atttgccgcg gatcgaaggg caccgccgcg
     1321 gcgtggccag cgtccgccgc acggtcgaag aagcggcccg cctccatttg gaacagctca
     1381 cgctctattg cctctccagc gagaactgga agcgccccca gcgggaaatc gactttttga
     1441 tgcacctcct cgagcagtac atgatcgagg agcggagcac gatcctgcgg cagaacatta
     1501 cggtccgcgt gatcggccgc cgcgaaggca ttcccgccgc cacgctggcc gagatggatc
     1561 gcaccgtcga aatgagcgcc gccaacaccg gcacgcggtt gtgcctggcg atcaactacg
     1621 gcggacgaag tgagatggtc gatgcggtga aacagatcgc tgccgaagca aagagcggcc
     1681 agctcgagcc cgcctccatc gatgaagcag ccatctcgga gcggctctat acggccggca
     1741 tgcccgatcc ggacctcctc atccgcaccg cgggcgaaat gcggatcagc aacttcctgc
     1801 tctggcagat cagctatgcc gagctgtggg tcaccgaccg ctgctggccg gaatttgaga
     1861 tcgccgattt tcatcaagca atccgcgatt tcgcctcgcg cgatcgaaaa ttcggcggga
     1921 tcaggccgta ggagtgtttc gtctttggtg tttcgtgttt tgtgactccc atcgctcaga
     1981 acgactcaca aaacacaaga aacgaaaaac aaaacactcg gagtgctccc ttgcttcgct
     2041 ggcgactcat ctcagccgcg gttatcgttt cggtcctgct cacgcttgtc tggctcgatt
     2101 tccggcgggc cgtcttcggc gtggcgggcg tatggctctt tcccgtgctg ctcgcggtca
     2161 cgtggatggc gaccgaggaa gtgctcggcc tcctgcgggc caaggggcac cggcccgtcg
     2221 cctggatcct ctatctcggc aacggcctga tcacgctggc ggcctgcctg ccgatgctgt
     2281 tcgagttctt cgaccggccc ttcccggacg acaatcccct cggccgcttc ggctggccgc
     2341 tggtggcgct ggcgattgcc acggtcggcg tcttcgcggc agaaatgcgg cggttcgagc
     2401 agccggggca aacgacggtc ctcgcggctc tgggcatttt cacgcttgtc ta
//
LOCUS       DASUGB010001186         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1521520, whole genome shotgun sequence.
ACCESSION   DASUGB010001186 DASUGB010000000
VERSION     DASUGB010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521520"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..514)
                     /locus_tag="VFV87_22490"
     CDS             complement(296..514)
                     /locus_tag="VFV87_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106613.1"
                     /translation="MRRLLLAAAFGLMLSANVGCFLPIYSGDPAVRMQELIFTSEDFR
                     NILEEWRRIWFLDQPSHMSVMRVHGGVM"
     gene            complement(648..1052)
                     /locus_tag="VFV87_22495"
     CDS             complement(648..1052)
                     /locus_tag="VFV87_22495"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5106614.1"
                     /translation="MPDDIQPEESTNVTKKEIVKTISEEIGLTQLKTKEIVQKTFDAI
                     VEALVEERRIELRNFGVFEVKVRAARKARNPRTGERVDVPEKSVVTFKPGKEMEERVR
                     QLEAARKARQAASAVHATADGNPQSTNQQASA"
     gene            complement(1500..1919)
                     /locus_tag="VFV87_22500"
     CDS             complement(1500..1919)
                     /locus_tag="VFV87_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5106615.1"
                     /translation="MANELPWYRDGLRFTCTQCGDCCTGAPGYVWVTNEEIAALAAQI
                     GESVARFEELYVRRVGARKSLREFPTGDCVFFDNQRRKCTVYDARPRHCRTWPFWDSN
                     LKSPADWAHTCDVCPGSGQGKLHEWSQIEVQRKSVKV"
     gene            complement(1960..>2409)
                     /locus_tag="VFV87_22505"
     CDS             complement(1960..>2409)
                     /locus_tag="VFV87_22505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106616.1"
                     /translation="LRDAASELAAASKLDLHNLAFCERVQSFGWYTEFPRNEFQPKQQ
                     VILYVEVDNFAAIEKGPNSFETQLQGHYQIFDGRGNIVAERKLPLDQEVCRNIRRDYF
                     LAYPIYMPESIAPGRYRLELTIEDLKASAQYQGRKFGEGMIEFSIRG"
BASE COUNT          474 a          744 c          690 g          501 t
ORIGIN      
        1 acggttttgg gaaggatcgc tcccaggcgg gagaaatcga ccagcccggc catctcgcgg
       61 gcgtagccga atgtgcccga ggcggtgaga atcggactgc ggagcgtgag ccgcccaagt
      121 tggacggaca ggtctgtctc cgctttaggg agcgagcaag cctcgccgcg cgcgttcatg
      181 ggctcgaatg tatcccgcgg ccgcgggagc ggcaacgccc ggagccgtcc cgaactggca
      241 gctcgccgcg gcgcaaaggc aggcagcgct gcgacttatg cggcaaatcc tgcggctaca
      301 tcacgccgcc gtgaacccgc atcaccgaca tgtggctggg ttggtcgagg aaccagattc
      361 gacgccattc ttccaggatg ttgcggaagt cttcggaagt gaagatcagc tcctgcattc
      421 gcacagctgg atcgccgctg tagatcggca ggaagcagcc gacgtttgcc gaaagcatca
      481 atccaaaagc cgctgccaga agaagccgac gcatcgatct ttcctccgtt aaaggatcgc
      541 ccgaggtgtt tcgcgattgg ccatggccga caacggccgc ggcgtaagtg attgtctttc
      601 cgtgacttgc gaaacaggac tcggttgctc cggcgtggcc ggacggttca ggccgaggcc
      661 tgttgattcg ttgactgagg gttaccgtca gcggtcgcat gtaccgctga cgccgcctgg
      721 cgggcctttc tggcggcctc caactgccgc acgcgctctt ccatttcctt gccaggctta
      781 aatgtgacga ctgacttctc gggtacatcc acgcgttcgc cggtgcgagg gttgcgggct
      841 ttgcgggccg ctctcacctt cacctcgaac acgccgaagt ttcgcagctc aatccgccgt
      901 tcctcgacca gcgcttcgac aatggcatca aacgtcttct gcacaatctc cttggtcttc
      961 aattgggtca gcccaatctc ctcggaaatc gtcttgacga tctctttctt ggtcacgttt
     1021 gtgctctcct ccggctgaat gtcatccggc accgcgggaa atcgctggca gtttccagca
     1081 attcgctcca agtgtagatg gcataacgac tagagtcaag ctcaagcggt ttttgttcgg
     1141 tcgtaagtcc aatgcttccg ttgacgaaag cgcaggtcgg aaggagtcgg cgtctggcaa
     1201 ggtgttaatc taagtataaa cgggcaaacg agttaagcgg acggccgccc gtgcagccaa
     1261 gtcatcctcc gtaaaccatt gccagctaag ggttaaagat cgatccgcgt agcctacctg
     1321 gaggccagcg cggttcaaca atcctccacc ataaatcggc cagaggcgcg gaaaactcca
     1381 aagattcggc aaactttccc caaccggctt gatcgttagg gtccgcagca taggtgggtg
     1441 ccctggtgcc cccgatcgtt gccacagtat tccgcagccc gcccggctcg tccgcccttt
     1501 tagaccttga ccgatttccg ctgcacctca atttgcgacc actcgtgcag tttcccctgg
     1561 ccgctgccag gacacacatc gcacgtgtga gcccaatcag cgggcgattt gagattcgag
     1621 tcccaaaacg gccaggtccg acagtgccgc ggccgggcgt catagacggt gcatttgcgc
     1681 cgctggttat cgaagaatac gcagtcgccg gtgggaaatt cccgcaagct cttacgggcg
     1741 cccacccggc ggacgtacag ctcctcaaaa cgtgcgaccg actcgccgat ttgggccgcc
     1801 agggcagcaa tctcttcatt cgtgacccat acatagcccg gcgcgcccgt gcagcagtcg
     1861 ccacactggg tgcatgtgaa tcgcaggccg tctcgatacc agggcagctc gttggccatt
     1921 ggcccattcc ttcggagata ctcgcggtaa acgcccattc tagccgcgaa tcgaaaactc
     1981 gatcatcccc tcgccgaact tccgcccctg gtactgcgcg gaagccttca gatcctcgat
     2041 cgtcagctcc aatcggtagc ggccaggggc gatcgactcg ggcatgtaaa tcggataagc
     2101 gaggaagtag tcgcggcgaa tgttgcggca cacttcctgg tccagcggca gcttccgctc
     2161 cgctacgatg ttccctcggc cgtcgaaaat ctggtagtgt ccctgcaatt gcgtctcaaa
     2221 cgagttcggg cccttctcga tggctgcgaa gttatcgacc tctacgtaca aaattacctg
     2281 ctgttttggc tggaattcat tgcggggaaa ttccgtgtac cagccgaagc tttgcacccg
     2341 ctcgcagaag gcgagattgt gcagatcgag cttgctagct gcggccagtt ctgacgccgc
     2401 atcccgcag
//
LOCUS       DASUGB010001187         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_689085, whole genome shotgun sequence.
ACCESSION   DASUGB010001187 DASUGB010000000
VERSION     DASUGB010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689085"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFV87_22510"
     CDS             complement(<1..233)
                     /locus_tag="VFV87_22510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106617.1"
                     /translation="MFNSIRWTMLGWQALILGLVVGGLGTALYRQVRHATYEQMDAEL
                     LGAAQAVALKIEAGQQPALPASFVARFGKVQERA"
     gene            complement(226..879)
                     /locus_tag="VFV87_22515"
     CDS             complement(226..879)
                     /locus_tag="VFV87_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5106618.1"
                     /translation="MRILIIEDEPDLLAALERMVHDEGYAVDTALDGREGLQKALDWE
                     YDAVVLDLMLPGLDGWELLKQLRKSKPVPVLILTARDAIRDRVQGLDLGADDYLLKPF
                     ERAELLARLRALIRRAAGQARSVIEIGDVLIDVAARKVTRQGKTVNLTAREYALVEFL
                     ALHRGQVVTRTMLYDHLFDENEDSLSNLLDVHVSNLRKKLGKDFVVTRRGRGYQIDV"
     gene            933..1319
                     /locus_tag="VFV87_22520"
     CDS             933..1319
                     /locus_tag="VFV87_22520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5106619.1"
                     /translation="MKRQADEWNVSAFRIHRVLGIAARNGIMLVSHFRHLEFEEGESF
                     GLPLVLRGAEERLAPILMTALTTGLVLVPLVIAGNLPGHEIEYPLAVVILGGLLTSTL
                     LNLFLLPPLYLFFARPTLGQAAGSHP"
     gene            1367..2938
                     /locus_tag="VFV87_22525"
     CDS             1367..2938
                     /locus_tag="VFV87_22525"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX5106620.1"
                     /translation="MVAVARSPLRIESWSGPVPRWLVSAVALDLRGLSVLRMGLGAIL
                     LWDLAVRALALSAHYTDAGLLPRRVRMGLIWDYNEPWWMSLHMLSGEVWWQGVLFAAA
                     AVAAVMLAAGYRTRWATIASWLLLLSLHGRNPLLLQGGDVLLRCLLFWSMFVPLGARW
                     GVDAWLAKRRGHQIGAPTSDPEIASAGTAAIIIQLACMYAFTALLKTSPAWRSDFSAV
                     HYALSIDHYTSRLGYELLRYPRLLATLTAGSWVLEGAGPLLLLSPIGRRWVRTIVPLA
                     MIGFHLGLALAMTLGTFPWICIAAWAALLPRPVWDALDRHLAPRNAAGSRSEATRWTG
                     SLVGNTAIACLLALVVVLNVKRLFNPLATVGGYPIGRTIQAAGLWQYWNMFSPGPYEY
                     GGWLRIEGQTADGRLVNLYQPDQPLPDAKPPLVSTMYPTQHWRRSIVTLWEYAEPSYR
                     ESVLRFFVRQWNEAHDTGEEVVQARLVQVIVPTQPPGSTALAPPERLFLAEWPEVGNN
                     RPPAGRINSAGSAGP"
     gene            3132..>4205
                     /locus_tag="VFV87_22530"
     CDS             3132..>4205
                     /locus_tag="VFV87_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106621.1"
                     /translation="MMHRSRMRLFFVALGVAAFALASQAQAQAPATLKEFAKGVVTTI
                     EPAPQDEEMFSGPRPLVEVPIEIEGLEYDPQLSPKTATVFERAKIATLRRTIWNLEFS
                     FKPMRMVYVDIPQPDGKMQRKLVWYMVYYVRNLGNHIRPKAVVEEVIAESPVTDANGK
                     VAGLTPGLVHVKYDKETTNEVEVFGKTTTELRFFPHFVLKSTEYKDESGKPKEYLDQV
                     IPAALEPIKAREFPGRDNVTLYNSLTISEVPIPVSDGTTDNRVWGVVTWVDIDPRIDY
                     YIVYVQGLTNAYQYEDPAGAFKKGDLPGSGRKIVRKTLQLNFWRPGDTVDPSEEEIRY
                     GCRIDSDEAEQQKIFAQFEIDKPI"
BASE COUNT          734 a         1307 c         1336 g          828 t
ORIGIN      
        1 gcgcgttcct gaaccttgcc gaatcgcgcc acaaagctcg cgggaagtgc cggctgctgc
       61 cccgcttcga tcttgagagc caccgcctgc gccgcgccga gcaattccgc atccatctgc
      121 tcgtaggtgg cgtgccgcac ctggcggtac agcgccgtcc ccagcccacc gacgaccagg
      181 ccgagaatca gcgcctgcca gccgagcatg gtccagcgga tggaattaaa catcgatctg
      241 atatccccgt ccgcggcgcg taacgacgaa gtccttgccg agctttttcc gcaggttgga
      301 aacgtggacg tcaagcaggt tcgagaggct gtcttcgttc tcgtcgaaca ggtggtcgta
      361 cagcatcgtg cgcgtgacca cctgcccgcg gtgcaacgcg aggaactcga cgagcgcgta
      421 ctcgcgggcc gtgaggttga ccgtttttcc ctgccgggtc accttgcggg ctgccacgtc
      481 gatcagcacg tcgccgatct caatcaccga ccgggcctgg ccggctgcgc gccggatcag
      541 cgcccgcagc cgcgccagca gttccgcccg ctcgaacggc ttgaggaggt agtcgtcggc
      601 gccgagatcg aggccttgca cgcggtcgcg aatcgcgtcc cgtgcggtca ggatcagcac
      661 cggcaccggc ttcgacttgc gaagctgctt gagcagttcc cagccatcga gtcctggcag
      721 catcaagtcg agaacgacgg cgtcgtattc ccagtcgagg gctttctgca gaccctcgcg
      781 tccgtccagc gccgtatcga ccgcatagcc ttcgtcgtgg accatccgct ctaaagcggc
      841 gagcaggtcc ggttcgtctt caataatcag gattcgcatg ggattcgctg cccctcgatt
      901 cgtcagccta gccgctgcga cctgaagcca aaatgaagcg tcaggccgat gaatggaatg
      961 taagtgcctt ccgaattcac cgggtgctgg gcatcgcggc ccgcaacggg atcatgctcg
     1021 tcagccattt ccgtcacttg gaattcgaag agggcgaatc tttcggcctg ccgctcgttc
     1081 tccgcggtgc ggaggagcgc ctcgctccga tcctgatgac cgccctcacc accggcctgg
     1141 tccttgtgcc gctggtcatt gccggcaacc tgcccggtca tgagatcgaa taccccctgg
     1201 ccgtcgtcat cctcggcggc ctcctcacca gcaccctgct caacctgttt cttctcccac
     1261 cgctctatct gttctttgcc cgaccgacgc ttggccaagc cgcaggatcg cacccgtgac
     1321 gtaggcttgt gacggatttg ctggccccgt ttctgagtcc tgaaacatgg tcgccgtcgc
     1381 ccggtcgccg ttgcggattg agagttggtc cggtcctgta ccgcgctggc tagttagcgc
     1441 ggtcgcgctc gatctgcgtg ggttgtcggt gctgcggatg gggcttgggg cgatcctgct
     1501 atgggatttg gccgtgcgag cgttggcctt gtcggctcat tacacggatg cgggcctgct
     1561 gccgcggcgc gtgcggatgg ggctgatttg ggattacaac gagccctggt ggatgagcct
     1621 gcacatgctg agcggcgagg tgtggtggca gggcgtgctg tttgcggcag cggcagtcgc
     1681 ggccgtgatg ctcgcggccg gctatcggac gcggtgggcg accattgcaa gctggctgtt
     1741 gctgctctcg ctgcacgggc ggaatccctt gctgctgcaa gggggcgatg ttttgctgcg
     1801 gtgtctgctg ttctggtcga tgtttgtgcc gctcggcgcg cggtggggcg tcgatgcgtg
     1861 gctggcgaaa cgccgcggcc atcaaatcgg agcgccgaca agtgatccgg aaatcgcatc
     1921 agcggggaca gccgcaatca tcatccagct cgcctgcatg tacgccttca cggccttgct
     1981 gaaaacgtcg ccggcctggc ggagcgattt ctcagccgtc cattacgccc tgtcgatcga
     2041 tcactacacg tcacgtttgg gatacgagtt attgcgatat ccgcggctgc tggcgacgct
     2101 gacggcgggc tcttgggtgc tggaaggggc cgggccactg ctgctgcttt cgccgattgg
     2161 ccgtcggtgg gtgcggacga tcgtgccgct ggcgatgatc ggctttcacc tgggtctggc
     2221 cctggcgatg accctcggca cgttcccttg gatttgcatc gcggcctggg cggctctgtt
     2281 gccgcggccc gtgtgggatg cgcttgatag gcatctcgct ccgcgaaatg ccgccggctc
     2341 gcgcagcgag gcgaccaggt ggaccggttc gctcgtcggc aacacggcga tcgcctgcct
     2401 gctggcgttg gtcgttgttt tgaacgtcaa gcgacttttc aacccgctgg cgacggtcgg
     2461 aggctacccg atcggtagaa cgatccaggc ggcgggtctc tggcaatact ggaacatgtt
     2521 ttcgccgggc ccctacgagt atggcggctg gctgcggatc gagggccaga cagcggacgg
     2581 ccggctggtc aatctttacc agcccgatca gccgctgccg gacgccaagc caccgctggt
     2641 cagcacgatg tatccgacgc agcactggcg gcggtcgatc gtgacgctgt gggaatatgc
     2701 ggagccgtcg tatcgcgaga gcgtgctgcg gtttttcgtc cggcaatgga acgaggccca
     2761 tgacacgggc gaggaggtcg tccaggccag gcttgtacag gtaatcgtgc ctacccagcc
     2821 gccgggatcg accgccctcg cgccaccgga gcgactgttt ctggccgaat ggcccgaggt
     2881 cgggaacaat cggccgcctg ccggtcgaat caatagtgcc ggttctgccg gcccctaagt
     2941 ggggcagaca tccttgcctg ccacgcttgg cgggcaagat agcctgctcc gcggcaactg
     3001 gcactttgtt gacccggcaa agtttccgta actatactgg ctaactagcc atttctgcgc
     3061 ggacgattcc cgaccgcgcg ccggcctccg cgcagcgacg taagtcgtta cgcgacagat
     3121 gagtacgcaa gatgatgcac cgtagccgaa tgcgtttgtt cttcgtggcg ctgggcgtgg
     3181 ccgcctttgc actggcttcg caagcgcaag cgcaagcacc cgcaacgctc aaggagttcg
     3241 ccaagggcgt cgtcaccacg attgagcccg cgccgcagga cgaagagatg ttcagcgggc
     3301 cgcgaccgct cgtggaagtg ccgatcgaga tcgaaggtct ggaatacgat ccgcagctct
     3361 cgccaaaaac tgcaacggtc tttgagcggg ccaagatcgc cacgctccgc cgcacgatct
     3421 ggaacctgga attctccttc aagccgatgc gaatggtcta tgtcgatatc ccgcagcccg
     3481 acggcaagat gcagagaaag ctggtctggt acatggtcta ttacgtccgc aacctgggta
     3541 atcacatccg gcccaaggcc gtggtcgagg aagtcatcgc cgaatcgcct gtcacggatg
     3601 ccaacggcaa ggtcgccggc ctgacgccgg gtctggtgca tgtaaagtat gacaaggaaa
     3661 cgaccaacga agtcgaggtc tttggcaaga ccacgaccga gctgcggttc ttcccgcatt
     3721 tcgtactgaa atcgaccgag tacaaggacg agagcggcaa gcccaaggag tatctcgacc
     3781 aggtgattcc cgcggctctg gagccgatca aggcccgtga attccccggg cgagacaacg
     3841 tcacgcttta caactcgctg acgatcagcg aagtgcccat tccggtcagt gacgggacga
     3901 ccgacaaccg cgtgtggggc gtggtgacgt gggtcgatat cgatccgcgg atcgattact
     3961 acatcgttta tgtgcagggt ctgaccaacg cctaccaata cgaggatccc gctggggcct
     4021 ttaagaaggg cgatctaccg ggcagcggcc gcaagatcgt ccgcaagacc ctgcaactca
     4081 atttctggcg gccgggcgac accgtcgatc ccagcgaaga ggagatccgt tacggctgcc
     4141 gaattgattc tgacgaggcg gaacagcaga agatcttcgc ccagttcgag atcgacaaac
     4201 ccatt
//
LOCUS       DASUGB010001188         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1244809, whole genome shotgun sequence.
ACCESSION   DASUGB010001188 DASUGB010000000
VERSION     DASUGB010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244809"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFV87_22535"
     CDS             <1..950
                     /locus_tag="VFV87_22535"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEX5106622.1"
                     /translation="SPEAGLLAAKSGDEGQRRAALARWITAEQNPLPWRSMANRLWQY
                     HLGRGIVDTPSDFGRMGAPPTHPQLLDWLAAELRSGDQSLKRLHRLIVTSHTYRQSSS
                     AELGTRSAERSDELSLVPSSALRTPSSIDSDNRYLWHANRRRLQAEAVRDAVLLLAGR
                     LDGRMGGPSFQDFVVEHPEHSPHYEYQLHDPDDPRSHRRSIYRFLVRSQPQPFMATLD
                     CADPSQRVDKRSESLSALQALATLNNGFMVVMSRHWAQRLAREQPNLDAQIERAVHEV
                     FGRPATDSELLLLTEYARDHGLANLCRALLNANEFAFVD"
     gene            975..1211
                     /locus_tag="VFV87_22540"
     CDS             975..1211
                     /locus_tag="VFV87_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106623.1"
                     /translation="MIDAIVAQREETHVPDAGTDDCRSSIDEVVAVYKKDVDRSMLRE
                     NLKLTPTERLERFEMIARQFAEIHEAGRRHRGEV"
     gene            1214..1726
                     /locus_tag="VFV87_22545"
     CDS             1214..1726
                     /locus_tag="VFV87_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106624.1"
                     /translation="MAKKLEPANLREILPMLHHGGVDFIVIGGGAAVVYGLARITLDV
                     DVVYSREIENIRRLVRAVAPSSPYPRDAPPGLPFRFDEGTLRLGMNFTLTTTLGHLDL
                     LGEVVGSGGYLELLPYTEVIESYGLPIRYVTLERLIHLKRAAGRPKDFEVLAELEALK
                     EERDRKGKQS"
     gene            1723..>2452
                     /locus_tag="VFV87_22550"
     CDS             1723..>2452
                     /locus_tag="VFV87_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106625.1"
                     /translation="MTRALCRASVSRRELLASVGQGFGGLALAALLGNDLAAAPTSDL
                     RPPTSLHHLPRAKRVVQLFMAGAASAIDLFDYKPELVKRHGQPSDFGEPVEAFQNGLG
                     PWLKPLWEFQPYGQCGRLLAEPVAALGAVADELAFVHNVVGKTGVHSQATLLQTTGFQ
                     LPGFPGMGAWVSYGLGSMNDNLPTFVVLPDHRGLASNGTKNWDAAFLPGQHSGTVIYP
                     GKSAPIADLFPHQRGDYITPQSEAA"
BASE COUNT          468 a          802 c          744 g          438 t
ORIGIN      
        1 cttcaccgga agcaggtttg cttgcggcta agtccggcga cgaagggcag cgccgagcgg
       61 cgctggcccg ctggatcacc gccgagcaaa acccgctccc ctggcggagc atggccaacc
      121 gcctctggca atatcacctt ggccgcggca tcgtcgatac gcccagcgac ttcggccgca
      181 tgggcgcccc gccgacccac ccgcagctgc tggactggct ggccgccgag ctgcgcagcg
      241 gcgaccaatc gctgaaacgt ctgcaccggc tgattgtgac gagccatacc tatcggcagt
      301 cgtcgagcgc ggagctcgga actcggagcg cagaacgcag cgacgaatta tccctcgttc
      361 ccagttccgc gctccgcact ccgagctcga tcgactccga caaccgctac ctctggcacg
      421 ccaatcgccg gcgactgcag gccgaagcgg tgcgcgacgc ggtcctgttg ctcgccggcc
      481 gcttggatgg ccgcatggga gggcccagct ttcaggactt cgtggtagag caccccgaac
      541 actcgcccca ctacgagtat cagctccacg accctgacga cccgcggtcg caccgccgca
      601 gcatttatcg cttcctcgtc cgttcgcagc cgcagccctt catggcgacg ctcgactgcg
      661 ccgatccgtc gcagcgggtc gataagcgaa gcgagtcgct ttcggcattg caggccctgg
      721 cgacgctcaa caacggcttc atggtcgtca tgagccgcca ctgggcccaa aggctggcgc
      781 gcgagcagcc aaacttggac gcccaaatcg agcgggccgt gcacgaagtg ttcggcaggc
      841 ctgcgacgga cagcgagctg ctgctgctga ccgagtacgc ccgcgaccat ggcctggcga
      901 atctgtgccg ggcgctcttg aatgccaatg aatttgcgtt cgtggattag aatggaacaa
      961 cggagcaaat acccatgatc gacgccatcg tagcgcagcg tgaagaaacg cacgtccctg
     1021 atgcgggcac cgacgattgc cgtagttcta tcgatgaggt cgtggcggtt tataagaagg
     1081 atgtggatcg cagcatgctg cgggagaatc tcaagctcac tcccaccgaa agactggagc
     1141 ggtttgagat gatcgcgcgg caattcgccg aaattcatga agcaggacgc cgccaccgag
     1201 gcgaggtctg accgtggcaa agaagctgga gccggccaac ctgcgcgaaa tcctgccgat
     1261 gctgcatcac ggcggcgtcg atttcatcgt cattggcggc ggcgcggcgg tagtctatgg
     1321 cctggctaga atcaccttag acgtggatgt cgtctatagc cgtgaaatcg aaaatatccg
     1381 gcgactagtc cgcgctgtcg ctcccagttc tccttatccg cgcgatgcgc ccccgggact
     1441 gccgtttcgc tttgacgaag gcacgctgcg ccttggtatg aatttcacgc tgacaacgac
     1501 gttgggccat cttgacttgc tcggcgaagt ggttggaagc gggggttatc tcgaactctt
     1561 gccttacacc gaggtaatcg aatcttatgg actcccaatc cgatatgtga cgcttgaacg
     1621 attgattcac ttgaaacgcg ctgcggggcg ccccaaagat ttcgaagtgc tcgcagagct
     1681 cgaagcattg aaagaggagc gcgaccgaaa gggcaaacag tcatgacacg agccctttgt
     1741 cgggcctcgg tctcgcggcg cgagctgctg gccagcgtcg gccaaggctt tggcggcctg
     1801 gcgttggccg cgctgctcgg caacgacctc gcagccgcgc caacttccga tctccgacct
     1861 ccgacctccc tgcaccatct gccccgcgcc aagcgcgtcg tgcagctgtt catggccggg
     1921 gcggccagtg cgatcgactt gttcgactac aagccggagc tcgtcaagcg gcacggccag
     1981 ccgagcgatt ttggcgaacc ggtcgaggcg tttcagaatg ggctaggccc ttggctcaag
     2041 cccctgtggg aattccagcc ctatggccag tgcggccgct tgctggccga gccggtggct
     2101 gcgctcggcg ccgtggccga cgaactggcc ttcgttcaca acgtcgtcgg caagaccggc
     2161 gtccacagcc aggccacgct cttgcaaacc accggctttc aactccccgg atttcccgga
     2221 atgggagcgt gggttagcta cggcctggga agcatgaacg acaacctgcc gacgttcgtc
     2281 gtgctgcccg atcaccgcgg cctggcgagc aacggcacca agaactggga cgcggccttt
     2341 ctgcccggcc aacacagcgg cacagtcatt tacccgggta aatccgcgcc catcgccgac
     2401 ctctttccgc accagcgagg cgactatatc actccacaaa gcgaagctgc cg
//
LOCUS       DASUGB010001189         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1939502, whole genome shotgun sequence.
ACCESSION   DASUGB010001189 DASUGB010000000
VERSION     DASUGB010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939502"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFV87_22555"
     CDS             complement(<1..527)
                     /locus_tag="VFV87_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106626.1"
                     /translation="MSAPFDPYHKWLGIPPEEQPPHHYRLLGIKPLESDPDVIESAAD
                     QRMVHLRSFQAGQYGALSQKLLNEVSAARLCLLEGTKKAAYDAQLRGQLAAGQLAAGQ
                     PAAKQPALPRAKAISAQASPAISAPAMPQINMAATRPRPSAPAAGQGGLPIWVLVPAG
                     AGGLCVVLLVVALIA"
     gene            1099..>2425
                     /locus_tag="VFV87_22560"
     CDS             1099..>2425
                     /locus_tag="VFV87_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106627.1"
                     /translation="MSGYKYMVPSDRLASNKVEEARFRALVQSILLSRTPLEENRANF
                     DAYYTWFAFPMMTQVSTEALQLLPERRNKFLRTELEVCSDARVHAHLVSLTLDQMSRV
                     VQDNFHPAVRYNAMLLISCLNDQEVVRNGSERRLPEPMSRALPFILDQFRKPENSDAI
                     KVAALIGLSRHLEWDPHRLDPATAIPAGLRTEIVNELLALAQMKDPPEGRDSEGQLWF
                     RRRAIEALGFACFAKSDPGVAAAMDKLLTDESEPVSLRLTVAAAMGRMNYQAPVKLDT
                     LATVKQLGYLALLACDDELNRVTKLKETETERVARIRGEARTSSSGGGDGMDLYASGS
                     DAASMETPDSPGGMYSPMGMGAGGYGAGGTGSVTLQDPKAYRFDFVRRKIRAYLYCVQ
                     LGLTGGDDFDPQRTAVIPATAANDAKPPSRGMYAFAKPGEEKKYASEVYG"
BASE COUNT          488 a          744 c          739 g          454 t
ORIGIN      
        1 atcgcaatca gcgcgacgac cagcaaaacg acacacaagc ctcccgcacc agccgggacc
       61 aagacccaaa tcggcagccc gccttgcccg gctgccggcg ccgagggccg cggccgcgtc
      121 gccgccatgt tgatctgcgg catcgcgggc gcactgattg cgggcgaggc ctgcgcggaa
      181 atcgccttcg cccgcggaag cgccggctgc ttcgccgccg gctgtcccgc ggcaagctgg
      241 cccgcggcaa gctggccgcg caattgagcg tcgtacgccg ccttcttcgt tccctcgagc
      301 aggcacagcc gcgcagccga gacctcattc agcagcttct gcgagagcgc cccgtattgc
      361 cccgcctgga acgagcgcag gtggaccatc cgctgatccg ccgcactctc gatcacgtcc
      421 ggatcgctct ccagcggctt gatccccagc agccgatagt gatgcggcgg ctgctcctcg
      481 ggcggaatgc cgagccattt gtggtaggga tcgaacgggg cagacatcgg atgttttcca
      541 ggcggagcgg cacctatcga tggaagcgta ttattgtccg cattagcccg ctccgccgcc
      601 agaagaagat tggccagttt tgacaatgcg atccgcagag cgtggttgac ttgagttgaa
      661 actcattgac ttttccccac aatcccgtgt actttaggaa gatgcggtat gtgcggcggc
      721 cagcccttat ctgacagcct cccggatcgg ggaggcggcg tcgccaggcg gcagccgtac
      781 cgcaccggaa caggggttag cccggcccga tcgaacttcg atgtgcagtt cgtcgagctg
      841 cgctgacgac acggctgcca cgatatgaag ttagggctga agtctgcgcc ctaacgatac
      901 ggcagcagga ggatcgacat gagtggaatt cccgttcgcg aaacgtcatg tttcgccgca
      961 atcgttgtac gtctccttgg gatcgtagcg gtcatacttt gtgtggcacc cgctacgaac
     1021 atctgctggg gccaggcgcc gcccgccgct ccagcagctt ctcccgccag aaccagcgcg
     1081 cccatcgcga ccgacgactt gagcggttac aagtacatgg ttcccagcga ccggttggcc
     1141 tccaacaaag tcgaggaggc cagatttcgg gcgctcgtcc agagtatctt gctctccaga
     1201 actcccttgg aggagaaccg ggcgaacttc gacgcctact atacgtggtt tgcgtttccg
     1261 atgatgaccc aggtgtcgac ggaagcgcta cagttgctgc cggaacgccg caataaattc
     1321 ctcagaacag agctggaagt gtgtagcgat gcgcgcgtgc acgcgcacct ggtgagtctg
     1381 actctggacc agatgagccg tgtcgttcag gacaattttc acccagcagt tcgttacaac
     1441 gcgatgctgt tgatcagctg cctcaacgat caagaggtgg taagaaatgg atcggaaagg
     1501 cgactgcccg agccgatgtc ccgggcgctg ccgttcattc tcgatcagtt ccgcaaaccc
     1561 gagaacagcg atgccatcaa ggtcgcggcg ctcatcggcc tgtcgcggca tctggaatgg
     1621 gatccccacc gactcgaccc tgcaacggca attccggcag ggctacgtac ggaaatcgtc
     1681 aacgaactgc tggcacttgc gcagatgaag gatccgccag agggtcgcga ttccgagggg
     1741 caactctggt tccgtcgccg tgcaatagaa gccttgggct ttgcctgttt tgcgaagtcg
     1801 gaccctgggg tggccgcagc gatggacaag cttcttacgg acgagagcga gcctgtgtct
     1861 ctgcgcttga ccgtggcagc tgcgatgggt cgcatgaatt atcaggcgcc ggtcaagctc
     1921 gatacgctgg ctaccgtcaa acaactgggc tatctcgccc tgctggcttg cgatgatgag
     1981 ttgaatcgag tcaccaagct caaggaaaca gaaaccgaac gggtggcccg gatccgaggc
     2041 gaagcccgta ccagcagctc tggcggcggt gatggaatgg acttatatgc ctcaggatca
     2101 gacgcggcga gcatggagac gccggattca cccggcggaa tgtacagccc catgggcatg
     2161 ggagctggcg ggtacggagc tggcggcacc ggaagcgtca ccttgcaaga ccccaaggcc
     2221 tatcgcttcg atttcgtgcg acgaaagatt cgcgcttacc tgtactgtgt gcaactcgga
     2281 ctcaccggcg gagacgattt tgatcctcaa cgaaccgcgg tcatccctgc caccgccgcc
     2341 aacgacgcga agcctccatc tcgcgggatg tacgcctttg ctaagcccgg tgaagagaaa
     2401 aaatatgctt cggaggttta cggcc
//
LOCUS       DASUGB010001190         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1522257, whole genome shotgun sequence.
ACCESSION   DASUGB010001190 DASUGB010000000
VERSION     DASUGB010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522257"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VFV87_22565"
     CDS             <1..1454
                     /locus_tag="VFV87_22565"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5106628.1"
                     /translation="GGVLMNSLHFPWLEAATIGPLLVAAVISRVRDSDRARQWSVIVT
                     AAVLVCTFGAWIEFALLGDGPGLGPRSFAISGRPLPVLAIDSLNAPLLPLTAFLYLLT
                     AVATLRTKIRRFSFVGFLISLSLALAVLGSLNPWAIIALLAVRTIAPYLELRARHKPT
                     RVYVLHMILFVSMLALGWWLVEREGLHGEHSFWAIMPLLIAVLVRTGSIPFHCWITDL
                     FEHATFGTALLYVAPMMGAYVAIRLLVPIAPAWVLQSISIISLTTAVYAGGMALVQRE
                     ARRFFCYILLSHSALVLVGLESLTPIGLTGALCVWLSVSLSLAGFGLVLRALEARHGR
                     LSLVAYHGVYEHTPLLAVCFLLTGMASVGFPGTFGFLGTELLVDGAVQAFPYIGIAVV
                     VAAALNGIAVVKAYFKLFTGTRHVSSVPLQVGTREKLVVLTLAALILGGGLIPSPAVE
                     SRHRAAEEILAERHSRIAPPATETAQNELPSEP"
     gene            1560..>3101
                     /locus_tag="VFV87_22570"
     CDS             1560..>3101
                     /locus_tag="VFV87_22570"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5106629.1"
                     /translation="MDKEKIEVTAADQLPFAQRITKDAIAGFLVFLIALPLCLAIAKA
                     SGYPPIGGVFTAIVGGILVSFISNSELTIKGPAAGLIVIVAGCVADFGGTFGKDAAAD
                     FSAYRMALAVGAVAAVLQILFGLFRAGKLGDFFPTAVVHGLLASIGVIIMVKSGLTML
                     GVTAKLPQENTHLIWEYPHFVTLYNLPIAIIGVTSLAIMIVYPMLKLKALKAIPSQVV
                     VLLVAIPLAMGLGLKQPSAEEVAAAQSTSAAAAQSASAAAATAAPAPAPAADGKTASE
                     HAPPPAEPIDPQHAITLSGKQWSLNPAKFLINVPSDFTKAFVFPDFTAFNRPAIAIKW
                     VVMFAIIASLESLLSAKAVDLLDPQKRKTDQNRDLFGCGMANLVASCIGGLPMISEIV
                     RSRANVDNGAQSRFANLFHGLFLLLFVSLLPFVVNMIPTAALAAMLVFTGFRLAHPRE
                     FAHMYKIGIEQFIVFVTTIVAIVCTDLLVGALIGLVVEMVVNVLNGLPIMSVFSSRPQ
                     ITESGD"
BASE COUNT          490 a          981 c          938 g          692 t
ORIGIN      
        1 ctggaggagt tctcatgaat tcgctccact tcccctggct cgaggcggcc acgatcggcc
       61 cgcttctcgt cgcggccgta atcagccgcg tgcgcgattc cgatcgagcg cggcagtggt
      121 cggtgatcgt tacggctgcg gtgctggtgt gtacgttcgg ggcctggatc gaatttgctc
      181 tgctcggtga cggaccaggg ctggggccgc ggtccttcgc catctcaggc cgccctctgc
      241 cagtgctggc aatcgactcg ctcaacgctc cgctgctgcc gctgacggcc tttctctatc
      301 tcttgaccgc cgtcgcgacg ctacgaacca aaatccgccg attctcgttc gtggggttcc
      361 tcatttcgct gtcgctggcg ctggccgtgc tgggcagtct caatccctgg gcgatcattg
      421 cgctattggc tgtcaggacg atcgccccgt acctggaatt gcgggcgcgc cacaagccga
      481 cgcgcgtgta cgttctgcac atgatcttgt ttgtttccat gctggccctg ggttggtggc
      541 tcgtggagcg cgaggggctt cacggcgaac actccttctg ggccatcatg ccgctcttga
      601 tcgccgtgct ggtgcgcacc ggcagcatcc cctttcactg ctggatcacc gatctgttcg
      661 agcatgcgac ctttggcacc gcgctgctct acgtcgcgcc gatgatgggc gcttatgtcg
      721 ccattcggct cctggtgccg atcgcgccgg cctgggtgct gcagagcatt tccatcatct
      781 cgctgaccac agccgtttat gctggcggca tggcgctggt gcagcgcgag gcgcggcggt
      841 tcttctgcta catccttctc agccattccg cactggtgct ggtcgggctg gaatcgctca
      901 cgccgatcgg gctgaccgga gccctgtgcg tgtggctttc ggtcagcctc tcgctcgccg
      961 gatttgggct ggtcctacgg gcgctggaag cccgccacgg ccgtctgtcg ctggttgcct
     1021 atcacggagt ttatgagcac acccccctgc tggcggtctg cttcctgttg accggaatgg
     1081 cgagcgtcgg cttcccgggc acgttcggtt tcctcggcac ggaattgctc gtggacggcg
     1141 cggtgcaagc gttcccttac atcgggattg ccgtcgtcgt ggcggcagcc ctcaacggca
     1201 tcgccgtcgt caaagcgtat tttaagctgt ttacggggac gaggcacgtc tcgtccgttc
     1261 cgctgcaggt cggcacgcgg gaaaagctgg tcgtcctgac gctggctgcg ttgatcctgg
     1321 gcggcgggct gattcccagc cccgccgtcg agtctcgaca ccgggccgcc gaggaaattc
     1381 tggccgagcg acacagccgg attgccccgc cagcaacaga aacagcccaa aacgagcttc
     1441 catcagaacc ttaacgcgcg ccactgggaa aaattacaaa cccgtcagcg gatttggcaa
     1501 atggcagaag ttcaccgaaa ttcggtgatc tgggaacaac catccttttg agcttcgcta
     1561 tggacaagga aaagattgaa gttaccgcgg cagaccagct tcccttcgct cagcggatca
     1621 ccaaggacgc cattgccggc tttctggtgt ttttgatcgc cttgccgctc tgtctggcga
     1681 ttgccaaagc gagcggctat ccgccaattg gcggcgtatt tacggccatc gtgggcggca
     1741 tcctggtttc gttcatcagc aactcggaac tgacgatcaa aggcccggct gccggtctga
     1801 tcgtcatcgt ggcgggctgc gtggccgact ttggcgggac gttcggcaag gacgcggcgg
     1861 ccgatttctc ggcgtatcgc atggccctgg ccgtcggcgc tgtcgccgcc gtgttgcaaa
     1921 tcctgttcgg cctgtttcga gcgggcaagc tgggcgattt ctttcccacg gccgtcgtcc
     1981 acgggctgct ggcttcgatc ggcgtcatca tcatggtgaa gagcgggctg acgatgctcg
     2041 gcgtcactgc caagctgccg caagaaaaca cccacttgat ctgggagtac ccgcatttcg
     2101 tcacgctcta caaccttcca atcgcgatca tcggcgtgac cagtctggcg atcatgatcg
     2161 tgtacccgat gctcaagctc aaagcgctca aggcgattcc gtcgcaggtg gtggtgctgc
     2221 tggtcgcgat tccgttggcc atgggtctgg ggctcaagca gccctcggcg gaagaggtcg
     2281 ccgcggcaca atctacttct gccgccgcgg cacaatccgc ttctgccgct gcggcgaccg
     2341 cggcgcccgc gccggcgccc gccgcggacg gcaagacggc aagcgagcac gccccgccgc
     2401 ctgccgagcc gatcgatcca caacacgcca ttaccctcag cggcaagcaa tggagcctca
     2461 atcccgcgaa gttcctgatc aacgtcccca gtgatttcac gaaggccttc gtgttccctg
     2521 acttcacggc cttcaatcgg ccggccatcg cgatcaagtg ggtcgtcatg ttcgcgatca
     2581 tcgccagcct cgagtcgctg ctcagcgcca aggccgtcga tcttctcgat ccgcagaaac
     2641 gcaagaccga ccagaaccgc gacttgttcg gctgcggcat ggcgaatctc gtcgcctcct
     2701 gcattggggg cctgccgatg atttcggaaa tcgtccgcag ccgggccaat gtcgataacg
     2761 gcgctcagtc ccgctttgcg aacttgtttc acggcttgtt cttgctgctg ttcgtgtcgc
     2821 tgctcccgtt tgtcgtgaat atgattccga cggctgctct ggcggcaatg ctggtgttca
     2881 ccggctttcg tctggcgcat ccgcgcgaat ttgcccacat gtacaaaatc gggattgagc
     2941 agttcatcgt cttcgtcacg acgattgtgg cgatcgtctg cacggacttg ctcgtcgggg
     3001 cactgattgg cctggtggtt gaaatggttg tcaacgtgct caacgggctg ccgatcatgt
     3061 cggttttctc gtcacgtccg cagataacag aaagtggaga c
//
LOCUS       DASUGB010001191         4828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1385256, whole genome shotgun sequence.
ACCESSION   DASUGB010001191 DASUGB010000000
VERSION     DASUGB010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385256"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..3579
                     /locus_tag="VFV87_22575"
     CDS             229..3579
                     /locus_tag="VFV87_22575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX5106630.1"
                     /translation="MPSICRFALAFLMLAAIPTATLRAVPGDAPPPPEEPQITAASDE
                     GQQALAGFKIPAGLKGEIWAAEPMLANPVAFCLDSRGRIYVCETFRQSRGIEDNRGHD
                     HWLDDDLAAMTIEDRLAYIQKHLGDKANDYTKHDDRVRLLEDTDGDGKADKQSIFADR
                     FNSILDGTAAGILVREGNVFLTCIPHLWLLRDEDGDGKAEVRKPLSTGFGVRFAFRGH
                     DLHGLVIGPDGRLYFSVGDRGLNVKTQEGTQLVNPESGAVLRCELDGSNLEIFATGLR
                     NPQELAFDEYGNLFTGDNNSDSGDQARWVHVVEGGDSGWRMAYQYLPDRGPFNREKIW
                     RPYDPVKHAATGVPEQPANIVPPITNFASGPSGLAYYPGTGLTDHFRGRFLLVDFRGG
                     PVNSGVRSFRVKPKGASFELIDAEETIWNVLATDVDFGPDGAIYLTDWVNGWNGEGKG
                     RIYRFHSPEAADSEIVKQVTALLAEGFNQRPIDELVGYLSHADRRVRQEAQFALVEKK
                     AAAPLSRVARESDSQFARLHAIWGLGQLARRKIDHANCLKQVVANLAGDTDAEVRAQA
                     AQVLGDLRHDSASDEVRRRLADESPRVRMSAAIALGKMHDRGAIAPLLSLLEEDANQD
                     PILRHAAVMGLAGAADGSPAALVAHAKHPSPAARLGVLLALRRLSSEQTALFLNDSDP
                     FVVIEAARAIHDVPIPAALPQLAALVTRGIQGDALIRRVLNANFRLGAPQNAAAIAAY
                     AARPDAPESMRLEALDMLANWAKPSNRDRVLGMWRPLAERPAAEAAAALRTNLAGIFS
                     GPDKVRAIAAQVAAKLEIKEVIPELCSLLADLNQPPQSRADALSALVSVKYAEIEPTA
                     RQSLTDQAPAVRAAARSVLAQLKVDDAVALLDRAIAGGECVERQSAFAALANLRGPAV
                     NELLAKSLDRLLAGEFPADAELDLISAVEPRLNRELRDKLREYQSRHRPDDPLAVWRA
                     CLIGGDAERGKKLFFERGQLSCVRCHKIGETGGDVGPELTKIAADKEKHREYLLESVV
                     TPSKTIAKNFETVVIQGVDGRQHTGILKQEDSEKLTLMTAEGQLVEILQADIEARKAG
                     KSSMPEDLTKHLTRHELRDLVEFLSALK"
     gene            3727..>4828
                     /locus_tag="VFV87_22580"
     CDS             3727..>4828
                     /locus_tag="VFV87_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106631.1"
                     /translation="MDLRLAIRAAAWALLACCAGLDLPAAEPATWHEAYPAALAEAQS
                     ESKLLLIVDVADDFTRVKADGPQAKLYRSLALADKRVAGLLAAHFVTAFRPVGPPACL
                     QLLSGDDETRPRPASRTASAAPGPPPDAGEYAIAYVCLPGERVLHCIPGFVTADELLK
                     ELQWAVEANDERLRAPPTEKLWFLRQRHLAAALPRHVEPFAELYESRWKKDFQAPATQ
                     SANDLATAIRVAQTVREHSLAARLKSVLPAGESREQFLKVLSAHGGLEMTLAHLVLSE
                     FPLPPLADLAQPLFETASGDHYWQSTPRRAELAEWWTEHGRAGAQRLIAVSDDPRYIA
                     AVRAPEPFKWPPTSPDVRPHLGHFATLHVSLDE"
BASE COUNT          850 a         1692 c         1487 g          799 t
ORIGIN      
        1 tgaacggcca ttaaatcctc aaagccgcga ccagtggtcg cggttcccat tgtcgtcgtt
       61 cgctctgcga acgaacgccc ttttgcggag cgaatggcga caatccgctt gccttgtcaa
      121 cctttcacaa ccgaactacg ctgaacggtc ccgccttaac tcccttctac caatgaacta
      181 cgccgtccgc gcggcgtcaa tctgccctct cgccgggctc ctcgtgccat gccttcgatc
      241 tgccgattcg cgctggcctt cctcatgctg gccgctattc cgacggccac gctccgggcg
      301 gtccctggcg acgccccgcc gcccccggag gaaccgcaga tcacggccgc ttcggatgaa
      361 ggccagcagg ccctcgccgg cttcaagatt ccagcgggcc tcaaaggcga aatctgggca
      421 gcggaaccga tgctcgccaa tcccgtcgcg ttctgcctcg atagccgggg tcgcatttac
      481 gtctgcgaaa ccttccgcca aagtcgcggc atcgaagaca atcgcggcca cgaccactgg
      541 ctggacgatg atctggcggc catgacgatt gaagatcggc tggcgtatat ccaaaaacat
      601 ctgggtgaca aggcgaacga ctacaccaag cacgacgacc gagtgcgcct gctcgaagat
      661 accgacggag atggcaaggc cgacaagcag tcgatcttcg ctgaccgatt caacagcatt
      721 ctcgacggca cggctgctgg cattcttgtc cgtgaaggaa acgtcttctt gacgtgcatc
      781 ccgcacctgt ggctcttgcg cgatgaagac ggcgacggca aagccgaggt gcgcaagccg
      841 ctgagcaccg gcttcggcgt ccgcttcgcc ttccgcggcc acgacctgca cggcctggtc
      901 atcggccccg acggccggct gtatttcagc gtcggcgatc gcggcctgaa cgtgaaaacg
      961 caggaaggca cgcagctcgt caatcccgag tcgggcgcgg tcctccgctg cgagctggat
     1021 ggctcgaacc tggagatttt tgccaccggc ctccgcaacc cgcaggaatt ggcgttcgac
     1081 gagtacggca atctcttcac cggcgacaac aacagcgaca gcggcgacca ggcccgctgg
     1141 gtccacgtcg tcgaaggggg cgacagcggc tggcgaatgg cctaccaata cctgcccgac
     1201 cgcggcccgt tcaaccgcga gaagatctgg cgcccttacg acccggtgaa gcacgccgcc
     1261 acaggcgttc cggaacaacc ggcgaacatc gtcccgccga tcaccaattt cgccagcggc
     1321 ccctccggcc tggcgtatta ccctggcacc ggcctgaccg accacttcag gggccgcttc
     1381 ctcctcgtcg atttccgcgg cggccccgtg aacagcggcg tccgctcctt ccgagtgaag
     1441 cccaagggag ccagctttga gctgatcgat gcggaagaga cgatctggaa cgtcctggcg
     1501 accgacgtcg atttcggccc ggatggcgcg atctatctca ccgactgggt caacggctgg
     1561 aacggcgaag ggaaggggcg catctaccga ttccactccc cggaagccgc cgatagcgag
     1621 atcgtcaaac aggtaacggc attgctcgcc gaaggcttca accagcggcc catcgatgag
     1681 ctggtcggct atctgtcgca tgccgaccgc cgcgtgcggc aggaggctca attcgcgctg
     1741 gtcgaaaaga aggccgccgc cccgctttcg cgagttgcca gagagagcga ctcgcagttc
     1801 gctcgcctgc atgccatttg gggcctcggc cagcttgctc gccgaaagat cgaccacgcc
     1861 aattgtctta agcaagtcgt ggcaaatctg gccggcgaca ccgatgccga agtgcgcgcc
     1921 caagcggctc aagtgctcgg cgacctgcgg cacgactcgg cgagcgatga agtccgccgc
     1981 cgactggccg atgaaagccc gcgcgtgcga atgtccgccg caatcgccct tggcaagatg
     2041 cacgaccgcg gagcgatcgc gccgctgctg tccctgctcg aagaagacgc caaccaggac
     2101 ccgatcctgc gacatgccgc cgtgatgggg ctggcgggag cggccgatgg atcgcccgca
     2161 gcgctggtcg ctcacgcgaa acatccttcg ccggcggccc gcttgggcgt gctgctcgcg
     2221 ttgcggcggc tgtcgtccga gcaaacggcg ctgttcctga acgattccga tccgttcgtc
     2281 gtgatcgaag cggctcgggc aattcacgac gtgccgatcc ccgcggcgct gccgcagctt
     2341 gcggcgctgg tcacgcgcgg gatccagggc gacgccctga tccgccgtgt gctgaatgcc
     2401 aacttccgcc tgggtgcgcc ccaaaacgct gccgccatcg ctgcctacgc cgcccgcccg
     2461 gatgcgccgg agagcatgcg cctggaagcg ctcgacatgc tcgccaactg ggccaagccg
     2521 tccaatcgcg accgggtcct cgggatgtgg cggcccctcg cagagcgccc cgctgccgaa
     2581 gccgcggctg cactgcggac caatctcgcg ggcatcttca gcggtccgga caaggtccgt
     2641 gcaatagccg cccaggtcgc cgcaaagctc gagatcaagg aagtgattcc cgagctgtgc
     2701 agcctgctgg ccgacctgaa tcagccgccg cagtctcggg ccgatgccct gtcggcgctc
     2761 gtgtccgtca agtacgccga aatcgagccg accgctcgcc aatcgctgac cgaccaggct
     2821 ccagccgtcc gtgccgccgc ccgcagcgtc ctcgcccagc tcaaagtgga cgacgccgtc
     2881 gctctgctcg accgggccat cgccggcggc gaatgtgtgg aacggcaaag cgcatttgcc
     2941 gccctcgcca atctccgcgg ccctgcggtc aatgaactgc tggccaaatc gctcgaccgg
     3001 ctgctggccg gtgagttccc cgccgatgcc gagctcgacc tgatctccgc cgtcgagccg
     3061 cggctgaatc gcgagctccg cgacaagctg cgcgagtacc agtcgcggca tcgccccgac
     3121 gatccgctcg ccgtctggcg ggcctgcctg ataggaggcg acgccgagcg cggcaagaag
     3181 ctgtttttcg aacgcgggca gctttcgtgc gtccgctgcc acaagatcgg cgagaccggc
     3241 ggcgatgtcg gccccgagct gaccaagatc gccgcggaca aagagaagca ccgcgagtac
     3301 ctgctggagt ccgttgtgac gccgagcaaa acgatcgcca aaaacttcga gaccgtcgtg
     3361 atccagggcg tcgatggccg ccagcacacc ggcatcctca agcaggaaga ttccgagaag
     3421 ctgacgctga tgaccgccga aggccaactt gtcgaaatcc ttcaggccga catagaagcc
     3481 cgcaaagccg gcaaaagctc gatgcccgaa gacttgacga agcacctcac tcggcatgag
     3541 ctgcgcgacc tggtcgaatt cctctccgcc ctgaagtgac cgccgcccag ccaccgcctg
     3601 cgccggaaaa aagcttgaca ttcggccggc atccgatacg ttgacggctg acgcccggcc
     3661 gaccgccagc tttttctggt cgcgccgggc tcgcggggcg ctaactcttc gattcgggcg
     3721 ggtttcatgg acctccgcct tgctatccgc gcggcagcgt gggcgctgct tgcttgctgc
     3781 gcggggctcg atttgccggc tgccgagccg gcaacctggc acgaggccta ccccgccgcg
     3841 ctggccgagg cacaatcgga atcgaagctg ctgctcatcg tcgatgtagc cgacgacttc
     3901 acgcgcgtga aggcagacgg cccgcaggcg aagctctatc ggtcgctcgc gctggccgac
     3961 aagcgagtcg ccgggctcct ggcggcccat tttgtaactg cgtttcgtcc ggtcggtccg
     4021 ccggcgtgtc tgcaacttct gtcgggggat gacgagactc gcccgcggcc tgcatcgagg
     4081 acagcgtctg ccgcaccagg tccgccaccg gatgccggcg aatatgccat cgcctatgtc
     4141 tgcctgcccg gcgagcgcgt gctgcactgc attcccggct tcgtcacggc agacgagttg
     4201 ctcaaggagt tgcaatgggc cgtggaagcc aatgacgagc gcctgcgggc gccgccgacg
     4261 gagaagctct ggttcctccg ccagcggcac ctggccgcgg ccttgccgcg tcatgtcgag
     4321 ccattcgctg aactctatga atcgcggtgg aagaaagact tccaggcccc tgccacgcaa
     4381 tcggccaatg atctcgccac agccatccgc gtggcgcaga cggtccgcga gcattcgctc
     4441 gccgcgcggc tcaagtccgt tctgcccgcc ggtgaaagcc gcgagcagtt tctgaaggtc
     4501 ctctcggccc acggcggact tgaaatgacg ctcgcgcatc tggtcctctc cgaatttccg
     4561 cttcccccct tggccgatct cgcccagccg ttgttcgaga cggcttcggg cgaccattac
     4621 tggcagagca caccgcgccg cgcggagctg gcggagtggt ggaccgagca cggccgcgcc
     4681 ggcgcgcagc gcctgatcgc ggtgagcgac gacccgcgct acatcgccgc ggttcgcgcc
     4741 cccgagccgt tcaagtggcc ccccaccagt cctgatgtcc gcccgcacct gggccacttc
     4801 gcgaccttgc acgtgtcgct cgacgagc
//
LOCUS       DASUGB010001192         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1522463, whole genome shotgun sequence.
ACCESSION   DASUGB010001192 DASUGB010000000
VERSION     DASUGB010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522463"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="VFV87_22585"
     CDS             <1..1738
                     /locus_tag="VFV87_22585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5106632.1"
                     /translation="LVASDDRAEAWAARRILERMDVALWRDQVLTSKNPRLLVEGGLA
                     LVIADPTRENALAVLEQVHKLMGGFVSDRDFIDMLRVMQVAIHRGQIQPDEIPGLKRQ
                     LAEEFPAGDPLMNRELARLLVYLQESSIIDRYLAYLKSDADDVDKLHLALHLRFLQSG
                     WTVDQRLALLSYYEEANQRKGGGSYARYVINATRDFCQGLNEDEAQKVVAQGHKWPNA
                     ALGALYRMPQRLDEPTLASLIALDEKLSAMKGDSIQRLQVGLVAVLARSGDVDSMTYL
                     RKVWDESPERRPVVAVGLAQHPSGENWSYVLRSLPVLEPLAATGVCRKLTDVEQAPEE
                     AEPYRQVILLGLKMRQKEPEKEDAAAPALDLLYFWTGEELATDQSEDKQLAAWQEWFA
                     GKFPNEPEAKLPVAAPTAKYSFDELLAHLSSDEAKGQASRGAAIFTRAQCIKCHRFEG
                     QGESFGPDLTAVANRFTRKELLESIVYPSHMISSQYASKTIRTTDGRTIAGLVIPGAA
                     GETVVVQPNGEKAVLAQGEIEEIKPSKTSSMPAGLLDPLTLEEIADLFTYLQGPARPG
                     ALSRRPVITEPK"
     gene            1946..2515
                     /locus_tag="VFV87_22590"
     CDS             1946..2515
                     /locus_tag="VFV87_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106633.1"
                     /translation="MTHWKTLLAASLATGFALWLAGCNPAAPPSSTNGSGSGATTDGH
                     GDEHGHEGHDHAHEHAHHGPHEGELMAIGEEEYHLEWKYGDQGKVTFYVLDGAAKEEV
                     PIAAEKIEVDVTIGENEPKTYELMAVNPSGDEMKTAQFEIGDEDFATGLKVLSSGKGG
                     GKAVIKSLTIGDKPFENLEIKVEEHDHAH"
     gene            complement(2543..>3451)
                     /locus_tag="VFV87_22595"
     CDS             complement(2543..>3451)
                     /locus_tag="VFV87_22595"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5106634.1"
                     /translation="GIKLPGLEFGSRMEYLTDRLTDEAVQFITSNANKPFFLYLPHYT
                     VHTPLQAKEELIARYQAKAKPDQPQHNATYAAMIASLDEGVGKIMTRLETLKLLDDTI
                     VVFTSDNGGLVLNQVTSNVPLRAGKGSAYEGGVRVPFIVSYPPRIKAGSTSDVPAMSI
                     DLLPTLIELCGLPEEESWPKWDGVSIAPALLQSGDVQREPLYWHYPHYHPGGATPYGA
                     IRAGDWRLVEFYEDGKVELYNLKDDRSEAKDLATSQPDKRDELLAMLRTWRKKVGAQM
                     PTPNPNFDPDRAAQAAGGAGKGKAKQ"
BASE COUNT          661 a         1125 c         1056 g          609 t
ORIGIN      
        1 gctggtcgct tcagacgacc gcgcagaagc ctgggccgcc cgccgcatcc tggagcggat
       61 ggacgtcgcc ctgtggcgcg atcaggtgct gacttcgaaa aacccacgac tgctggtcga
      121 aggcggcctg gccctggtca ttgcggatcc gacacgcgaa aacgccctcg ccgttctcga
      181 acaagtccac aagctgatgg gcggctttgt gagcgatcga gactttatcg acatgttgcg
      241 cgtgatgcag gtcgccatcc accgcggcca gatccagccc gatgaaatac ccggcctcaa
      301 gcgccagctc gccgaggaat tcccggcggg cgatcccctg atgaaccgcg agctggcccg
      361 cctgctcgtc tatctgcagg aatcgtcgat catcgaccgc tacctggcat acctcaaatc
      421 cgacgccgac gacgtcgata agctgcacct cgccctgcac ctgcggtttc tgcagtccgg
      481 ctggaccgtc gatcagcggc tggcactgtt gtcgtactac gaagaggcga accagcgcaa
      541 agggggcggc agctatgccc gctatgtcat caacgccacc cgagatttct gccaggggct
      601 gaacgaagac gaagcccaaa aggtcgtcgc tcaggggcac aaatggccca atgccgctct
      661 cggcgcgctg taccgcatgc cgcagcgact ggatgagccg acgctcgctt ccctcattgc
      721 gctcgatgag aagctctccg cgatgaaggg cgattcgatc cagcgcctgc aagtaggcct
      781 ggttgccgtg ctcgcccgca gcggcgatgt cgattcgatg acctacctgc gcaaagtctg
      841 ggacgaatcg cccgagcggc ggccagtcgt tgcggtcggc ctcgcccagc accccagcgg
      901 cgagaactgg agctacgtcc tccgcagtct gccggtgctc gagcctttgg ccgccaccgg
      961 cgtgtgccgc aagctgaccg acgtcgagca agctcccgaa gaagccgagc cgtaccggca
     1021 ggtgatcctg ctcggcttga agatgcgtca gaaagagccg gagaaggaag acgccgcggc
     1081 gcccgccctc gatttgctct atttctggac cggtgaagaa ctggcgaccg accagagcga
     1141 ggacaagcaa cttgccgcct ggcaggagtg gttcgccggg aagtttccga atgaaccaga
     1201 ggctaagctg ccggtcgcag cgccgaccgc aaaatattcc ttcgacgaat tgctcgccca
     1261 tctgtcgagc gacgaggcca aaggccaggc cagccgcgga gcggcaatct tcacgcgggc
     1321 gcagtgcatt aaatgccacc gctttgaagg acagggtgag agcttcggcc ccgacctgac
     1381 cgccgtcgcc aatcgcttca cgcgcaagga gttgctcgaa tcgatcgtct acccgtcgca
     1441 tatgatttcg tcgcagtatg cttccaagac gattcgtacg accgacggcc gcacgattgc
     1501 cggcctggtc attccgggcg cggcgggcga gaccgtcgtc gtgcagccca atggcgagaa
     1561 ggccgtgctt gctcaaggcg aaatcgagga gatcaagccg agcaagactt cgtccatgcc
     1621 cgccgggctg ctcgatccgc tcacgctgga agaaatcgcc gacctgttca cctacctgca
     1681 aggccccgcc cggcccggcg ccctctcccg ccgccccgtc atcaccgagc ccaaatgaag
     1741 tggggcaggc attcttgcct gccgtcagtg gggcaggcat tcctgcctgc cttccccgaa
     1801 cccgcccgcg gccggccgcg tagcaccacc agtcctggtg gggaccaggg gcgatatttc
     1861 ttttcggctt gggaatattg gctatccgcc cgggttcatc tgagtacacc cttcactcga
     1921 acctaacgca cacaaggagc agatcatgac ccattggaaa actctgttgg ccgcctccct
     1981 ggccacgggc ttcgccctct ggctggccgg ctgcaatccc gccgcccccc cctccagcac
     2041 gaatggcagc ggcagtggcg cgaccaccga cggccacggc gacgagcacg gccacgaagg
     2101 gcacgaccat gcgcacgagc acgcccatca cggcccgcac gagggcgagc tgatggccat
     2161 cggcgaggaa gagtatcact tggaatggaa atacggcgac caaggtaagg tgacgttcta
     2221 cgtgctggac ggcgctgcca aggaggaagt gccgattgcg gccgaaaaga tcgaagtcga
     2281 tgtcacgatc ggcgagaacg agccgaagac gtatgagcta atggccgtga atccctccgg
     2341 cgacgaaatg aagacggccc agttcgaaat cggcgacgag gatttcgcga ccggcctcaa
     2401 ggtcctctcc agcggcaaag gcggcggaaa agccgtcatc aagtcgctca cgatcggcga
     2461 caagcctttc gagaacctgg aaatcaaagt cgaagagcat gaccacgccc actagttcgc
     2521 acgggcgcgc cgtcagcgcg gactactgct tcgccttccc tttgcccgca ccacctgcgg
     2581 cctgagcggc gcggtcggga tcgaagttag ggttcggcgt cggcatttgg gcgccgacct
     2641 tcttccgcca ggtgcggagc atcgccagga gttcgtcgcg cttgtccggc tgcgatgtcg
     2701 caaggtcttt cgcctcactc cggtcgtcct tcaggttgta gagttcgacc ttgccgtctt
     2761 catagaactc caccagccgc caatcgccgg cgcgaatcgc gccgtagggc gtcgctccgc
     2821 ccggatgata gtgcggatag tgccagtaga gcggctcgcg ctgcacgtca ccgctttgca
     2881 gcagcgctgg agcgatgctc acaccatccc atttgggcca cgactcttcc tcgggcaagc
     2941 cgcagagttc gatcagcgtc ggcaacaggt cgatgctcat cgccggcacg tcgctggtcg
     3001 agccggcttt gattctggga ggatacgaaa caatgaacgg cacgcggacg cctccctcgt
     3061 aggccgagcc cttcccagcc cgcagcggca cgttgctggt cacctggttg agcaccaggc
     3121 cgccattgtc cgacgtaaag acgacgattg tgtcgtccaa gagcttcagc gtttccagcc
     3181 gcgtcatgat cttgccgact ccttcgtcca ggctggcgat catcgccgcg taggttgcat
     3241 tgtgctgcgg ctggtcgggc ttcgccttgg cctggtacct ggcgatcagt tcttccttgg
     3301 cctgcagagg agtgtggacc gtgtaatgcg ggagatagag gaagaacggc ttgttggcat
     3361 tcgaggtgat gaactgcacc gcttcgtccg tcagccggtc ggtgaggtat tccatccgcg
     3421 agccgaactc gagccccggc agcttgatcc c
//
LOCUS       DASUGB010001193         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_689970, whole genome shotgun sequence.
ACCESSION   DASUGB010001193 DASUGB010000000
VERSION     DASUGB010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689970"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFV87_22600"
     CDS             complement(<1..434)
                     /locus_tag="VFV87_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate carboxy-lyase"
                     /protein_id="HEX5106635.1"
                     /translation="MPFALHTLLDDLARSGHLVRLEDEIDARLEAAEIQRRVYARGGP
                     AVLYANVKGCRFPMVSNLFGTMERARYMFRHTWDSVRRAIELKIDASAALRNPLRYAW
                     SPLTAWQMQAKRVRSGPVLQHETKISQLPQLVCWPMDGGAFIT"
     gene            579..2039
                     /locus_tag="VFV87_22605"
     CDS             579..2039
                     /locus_tag="VFV87_22605"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5106636.1"
                     /translation="MPIAATTEPLTQVSADALVIGIHAEAPPTGFAVEFDRASGGLLS
                     RLIEAKEISGKKCEVVTLLAPAGVNAKQVVVVGLGSKDAFDRGTANRAASAAAKSLAA
                     KERGKVAFYLADGWLADVAEAAVCGSLVGCVGQDLYRSKKSRFPLADMAWAGVDAAVV
                     KRGEILADAVNLTRRLVNEPPSEMYPESFAAEAARVAEESGIKIEVWDQARLEAERCG
                     SLLAVARGSDREPRLVILRYSGGPSNQAPLALVGKGVTFDSGGLSIKPTDGMKTMKCD
                     MAGAATVVGAMQAIAKLKLPVNVIGLCGLVENMLSGKSYKLGDVLRSRSGKTIEVLNT
                     DAEGRLVLADVLDVCLQNKPAKIIDLATLTGACVVALGNDVAGLMTNDQPWADAVKSA
                     SDACGEPMWQLPMFPEYGEQIKSEVADIKNVGEGRWGGAITAGKFLEEFVQGKPWVHL
                     DIAGPAFLESSKSWLEAGGSGYGVRTLVEVARKGTA"
     gene            2260..2487
                     /locus_tag="VFV87_22610"
     CDS             2260..2487
                     /locus_tag="VFV87_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106637.1"
                     /translation="MVAFQLDAAHQRRLDELAKSTGQDGTALARQVLIDYLDFQALSG
                     DTPEAWAEASVALAPEIMEQEDWGEGDHGSK"
     gene            2474..2788
                     /locus_tag="VFV87_22615"
     CDS             2474..2788
                     /locus_tag="VFV87_22615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5106638.1"
                     /translation="MDRSEIWWADLPPPIGRRPVLVLTRSAAVPIRNQVVIAQVTTTA
                     HNIPAEVALTRADGMPRDCVVNCDVLMTVPKARLTSRITKLTLAKMAEVQRALKFAME
                     LS"
     gene            2827..3963
                     /locus_tag="VFV87_22620"
     CDS             2827..3963
                     /locus_tag="VFV87_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5106639.1"
                     /translation="MTKSLNIGMIGYGFMGRAHSNAYANVAHYFDTPYQPVFKAVCAR
                     DAEKAKAFADRWGYESIETDWKKLLARKDIDAVDICTPNNLHKEIAIAAAGAGKAILC
                     EKPLAMNSSEGEEMCQAVEKAGVANIVWYNYRRIPAVSFAKQIIDSGKLGKIFHYRAN
                     FLQDWTISADLPQGGAALWRLDVAAAGSGVTGDLLAHCIDTAIWLNGGVSNVTAMTET
                     FIKERKHNLTGKVEKVGIDDACAFLCRFDNGSLGLFESTRYARGHKALYTFEINGEHA
                     SLKWDLHDLHRLQWFDHRTEGPLRGWTSIHVTDGDHPYMGKWWVPGLAIGYECGFVHQ
                     VADFLTGLASGKPAGPTFRDALETQKVCDAVLASAKSGIWTNVR"
BASE COUNT          829 a         1288 c         1364 g          724 t
ORIGIN      
        1 gtgatgaagg ccccgccgtc catcggccag cacacaagct gcggcagttg gctgattttc
       61 gtctcgtgct gcagcaccgg gccggagcgg acgcgcttcg cctgcatttg ccacgcggtg
      121 agcggggacc aggcatagcg cagcggattc cgcagcgcgg cggaggcgtc gatcttgagt
      181 tcaatggccc gccgcacgct gtcccaggtg tggcggaaca tgtaccgcgc ccgctccatt
      241 gtgccgaaca gattcgacac cattggaaac cggcagccct tcacgttggc gtacagcaca
      301 gccggtccgc cgcgggcata cacccgccgc tggatttccg ccgcttccag gcgcgcgtcg
      361 atctcgtcct ccaggcgcac gaggtggcca gagcgggcga ggtcgtcgag gagggtatga
      421 agggcgaagg gcacgcgcct gatgggggac agtgaggtgg cggttataga tgggaggtaa
      481 aagatggaag cattggtact cgctacgatg accgtggcgt gagcagtaga ataacggtcc
      541 ccattcatcc ggcctaccca cttctcctta tgtctcccat gccaatcgcc gccaccaccg
      601 agccacttac ccaagtctca gccgatgccc tcgtgatcgg aattcacgct gaagcacccc
      661 ccacaggctt cgctgtggag ttcgaccggg ccagcggcgg actcttgtcg cgtttgatcg
      721 aagcgaagga aatcagcggc aagaagtgcg aagtggtcac gctgctggcg ccggcgggcg
      781 tcaacgcgaa gcaggttgtc gtggtgggtc tgggctcgaa ggatgcattc gaccgcggca
      841 cggcgaatcg cgcggccagc gccgccgcga agtcgctggc cgccaaggaa cgcggcaagg
      901 tcgccttcta tctggccgac ggctggctgg cggatgtcgc cgaagcggca gtttgcggca
      961 gcctggttgg ctgtgtgggg caagaccttt accgctccaa gaagagccgt tttccgctgg
     1021 ccgatatggc ctgggccggc gtcgatgcgg cggtggttaa gcgtggcgaa atcctggcag
     1081 atgcggtcaa tctcacccgc cggctggtca acgagccgcc gagcgagatg tatcccgagt
     1141 cgttcgctgc cgaagcggcc cgcgtggccg aggaatccgg catcaaaatc gaagtttggg
     1201 accaggcccg cctggaagcc gaacgctgcg gctcgctgct cgccgtcgcc cgcgggtccg
     1261 accgcgagcc gcggctggtg attctgcgct atagcggcgg cccatcaaat caggccccgc
     1321 tcgcccttgt tggcaagggc gtgacgttcg acagcggcgg cctctcgatc aagccgaccg
     1381 acgggatgaa gacgatgaag tgcgacatgg ccggcgcggc gacagtcgtc ggcgcgatgc
     1441 aggcgatcgc caaattgaaa ctgccggtga acgtgatcgg cctctgcggc ctggtcgaga
     1501 acatgctcag cggcaagtcg tacaagctgg gcgacgtgct acgctcccgc agcggcaaga
     1561 cgatcgaggt gctcaacacc gatgccgaag ggcggctggt gctggccgac gtgctcgatg
     1621 tctgcctgca gaacaagccg gcgaagatca tcgacctggc gacgctgacc ggggcgtgcg
     1681 tcgtggccct ggggaacgac gtggccggcc tgatgaccaa cgatcagccc tgggccgacg
     1741 cggtgaagtc cgccagcgac gcctgcggcg agcctatgtg gcagctcccg atgtttcccg
     1801 aatacggcga gcagatcaaa agcgaagtgg ccgacatcaa gaacgtcggc gaaggccgct
     1861 ggggcggagc aatcaccgcc ggcaaattcc tggaggaatt cgtgcagggc aagccctggg
     1921 tccacctcga catcgccggc cccgccttcc tcgagtcgtc caaatcctgg ctcgaagccg
     1981 gcgggagcgg ttatggcgtt cgaacgctcg tggaagtggc aaggaagggg accgcatgag
     2041 atgaagctca gtgtccttcc catttgtgga tgtctaatcc tcgctggctg cggaggattt
     2101 tgacggaggc gcggacaaac tcgctgcgat ctgcaaggaa ctgttcccgg acgacccaga
     2161 aattcaggca atgaaaccgt aggaatgacc tcgtcccgca accgatgcaa gaggcgcgct
     2221 gtaggttaga attgaggtag ttctttcgga gtctcaccca tggtcgcatt tcaactcgac
     2281 gctgcccacc aacgacgact ggatgaacta gccaagtcca ctgggcaaga tggcactgcg
     2341 ctggctcgac aagttctcat cgactatctc gatttccagg cactttccgg cgatactccg
     2401 gaagcctggg cggaagcatc ggtcgctctg gcacccgaga ttatggagca ggaagactgg
     2461 ggcgaaggcg atcatggatc gaagtgaaat ctggtgggcc gacttgccgc cgccaattgg
     2521 ccggcggcca gttctagttc tgacacgatc cgccgccgtc ccgattcgca atcaggtcgt
     2581 catcgcccaa gtgacgacta cggcgcacaa catcccggct gaggtcgcac tcacgcgtgc
     2641 cgatggcatg ccccgcgatt gcgttgtaaa ttgcgacgtg ctcatgacgg tccctaaggc
     2701 ccgcctgacg agccgcatca ccaaactgac tctcgcgaag atggctgagg tgcagcgggc
     2761 actcaaattc gcgatggaac tgtcctgata cttcgccacg gcgagcgcga tggagaatct
     2821 tgacgtatga ccaaatctct caacatcggc atgatcggct atgggttcat ggggcgggcg
     2881 catagcaatg cgtatgcgaa cgtggcccat tatttcgaca cgccgtatca gccggtgttc
     2941 aaggcagtgt gtgcccgcga cgcggagaag gcgaaggcgt ttgccgaccg ctggggctat
     3001 gagtcgatcg agaccgattg gaagaagctg ctggcgcgga aggacatcga cgcggtcgat
     3061 atctgcacgc cgaacaactt gcacaaggaa atcgccatcg ccgccgccgg cgccggcaag
     3121 gcgatcctct gcgaaaagcc gctggcgatg aactcttccg aaggcgagga aatgtgccag
     3181 gccgtcgaga aggccggcgt ggcgaacatc gtctggtaca actaccgccg catcccggcc
     3241 gtcagcttcg ccaagcagat catcgacagc ggcaagctcg gcaagatctt ccactaccgg
     3301 gccaactttt tgcaggattg gacgatcagc gccgatctgc cgcaaggcgg ggccgcgctg
     3361 tggcggctgg atgtggccgc tgccggcagc ggcgtcacgg gcgatcttct ggcccactgc
     3421 atcgacacgg cgatctggct caacggcggc gtcagcaacg tcacggcgat gaccgagacc
     3481 ttcatcaagg agcggaagca caacctgacg ggcaaggtcg agaaggtcgg catcgacgac
     3541 gcctgtgcct tcctctgccg cttcgacaac ggctcgctgg ggctgttcga gagcacgcgg
     3601 tacgcccgcg ggcacaaggc cctctacacc ttcgagatca acggcgagca cgccagcctc
     3661 aagtgggatc tgcacgatct gcaccgcctg cagtggttcg accaccgcac cgaaggcccg
     3721 ctccgtggct ggaccagcat tcacgtcacc gacggtgacc acccctacat gggcaagtgg
     3781 tgggtccccg gcctggccat cggctacgaa tgcggcttcg tccatcaggt cgccgacttc
     3841 ctcactggcc tggccagcgg caagcccgcc ggcccgacgt tccgcgacgc cctcgaaacg
     3901 cagaaagtct gcgacgccgt cctggccagc gccaagagcg ggatctggac gaatgtgcgg
     3961 tagcgattgc ggttcgcctc acttcggcag gcactataga atggggcgaa gtgacaactc
     4021 gctgccagga gaaccgttat gattgcacac attggcgaac aaggcttgct gattcccaaa
     4081 gagctgcttg gggatgcaaa ggaagccgag attcacgcgc agcgcggccg acttgtcatc
     4141 gtgctcgatc cggccgatga tccgattcgg cgtctgggca agaatcccgt gattgcgccg
     4201 gagtc
//
LOCUS       DASUGB010001194         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_412224, whole genome shotgun sequence.
ACCESSION   DASUGB010001194 DASUGB010000000
VERSION     DASUGB010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412224"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFV87_22625"
     CDS             complement(<1..510)
                     /locus_tag="VFV87_22625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5106640.1"
                     /translation="MLEAQRGEWSRWRGPNGDGISYETGLLDSWPDEGPPLAWRVKGL
                     GGGYASVAVAGGKIFTLGNKQGKNQIVCLNVDDGSLLWSTPIGGGGGPNCTPTVDGDL
                     VFALGIEGDLACCKTDDGSLVWSKNYKRDFGVDGKPGWGFSESPLVDGDRLICTPGNN
                     DSVLACLDKR"
     gene            1030..1494
                     /locus_tag="VFV87_22630"
     CDS             1030..1494
                     /locus_tag="VFV87_22630"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5106641.1"
                     /translation="MDFQLHLETESVESCYPAKPLCAAPDDTIRAVLRKLRDTRTGAA
                     MICVDEKLVGIFTERDALKILADGSSLDAPVSSIMARNPVTLLKDDTVGKAIALMSAG
                     GFRRVPIVDEDGKVTGLVKVSGILHYLVEHFPKVVYTLPPAPHHKTTTREGA"
     gene            1607..>2528
                     /locus_tag="VFV87_22635"
     CDS             1607..>2528
                     /locus_tag="VFV87_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5106642.1"
                     /translation="MSTTTVEPAPQTHKDVQRIPSATVRFAGDSGDGMQLTGEQFTRT
                     SALLGNDVSTFPDFPAEIRAPRGTLAGVSGFQVHFASTDIFTPGDTLDALVVMNPAAL
                     KTNLTDLRKGGVLIANADNFEKKDWEMAGYDENPLDAEVLEHTYQLYKVPMTKIVREA
                     LKDMGMSQKEVDRCKNFFALGLVYWLYGRDMDPTLRFLNAKFATKPEIVESNTRALKA
                     GWNYGETTDAFASTYKVEKAKLPPGTYKNIMGNTALAWGLVTAAHLSGKELFLGSYPI
                     TPASDILHELAKFKNFGVRTFQAEDEIAAIC"
BASE COUNT          524 a          790 c          763 g          451 t
ORIGIN      
        1 gcgcttatcg aggcaggcca ggaccgaatc gttgttgccg ggcgtgcaga tcagccggtc
       61 gccatcgacc aggggcgatt cgctgaagcc ccagccgggc ttgccatcga cgccgaaatc
      121 gcgcttgtag tttttcgacc agaccaggga gccgtcgtcc gttttgcagc aggcgaggtc
      181 gccttcgatt cccagggcga agaccaggtc gccatcgacg gtgggcgtgc agttgggccc
      241 gccgccgccg ccgatcggtg tgctccagag gagcgagccg tcatcgacgt tcaggcagac
      301 gatctggttc tttccctgct tgttgcccag ggtgaagatc ttgccgccgg caaccgcgac
      361 ggaggcatag ccgccgccga ggcctttgac gcgccaggcc agcggcggac cttcatccgg
      421 ccaggagtcc aaaagaccgg tctcatacga gatgccatcg ccgttagggc cgcgccagcg
      481 ggaccactcg ccgcgctgcg cttccaacat tgctggattg ttagctgccg aaccggaaga
      541 tttgctcttg gagcgggcgg accttgacgt ttgggcttcg acagatgaat tacaggccag
      601 gcacgccgcc agaaggaccg ccgcgagcga cgccagtcca aaccaaatga atctcctcat
      661 gttcaatcac tccaacaagt tgggtcgcca gcgggtggtg gttgatatca acgctggctg
      721 attataggcg gaaatactcc gtcaaccagt tcaattgcaa gtggcccgag ccgcgatagt
      781 cgttacggac caaacaccag cggccattct gtcaggcgag gcggcgagcg gccgaagaat
      841 gcagttagcg ggaccgtcgg gcggacagct accttgttcg agaccccatc ggctggtagg
      901 ctggggggaa gtatgacgat tgtgcctacc acgcgagcta agggatttgg tttccctgta
      961 gccgcgatca agggctgagc cgacgccgcg aaggccgtct tcccgccgtc agccctttca
     1021 ggttgtgccg tggacttcca gctccatctc gaaaccgagt cggtcgagag ttgctatccg
     1081 gccaaaccgc tatgtgctgc gcctgacgat acgatccggg ccgtgcttcg caagctgcgc
     1141 gacacgcgga ccggcgccgc gatgatctgc gtcgatgaaa agctggtggg aatcttcacg
     1201 gagcgcgatg ccctgaagat cctcgccgac ggcagcagtt tggatgcacc ggtatcgagc
     1261 atcatggctc gcaaccccgt gaccctgctc aaggacgaca cggttggcaa ggcaattgcc
     1321 ctgatgagcg ccggcggctt ccgccgcgtg ccgatcgtcg atgaggacgg caaagtgacg
     1381 gggctggtca aggtgtccgg cattttgcac tacctggtcg agcacttccc gaaagtcgtc
     1441 tatacgcttc cacccgcccc gcaccacaag acaacgactc gtgagggcgc ttgatgccag
     1501 ccagcgccaa acgaaatcgg atttgtcccc cacctaaaca gcgaaataat tgactgccgc
     1561 gcttcgcaag agcgaagctt ccgccaatcg aggaacccgg gcgccaatgt ccaccaccac
     1621 cgtcgaacct gccccacaaa ctcacaaaga cgtacaaagg atccccagcg ccacggtccg
     1681 ctttgccggc gactccggcg acggcatgca gctcaccggc gagcagttca cgcggacgag
     1741 cgcgctctta ggtaacgacg tttcgacatt tcccgacttt cccgccgaaa tccgcgcccc
     1801 ccgcggcacg ctggccggcg ttagcggctt tcaggtgcac tttgccagca ctgatatctt
     1861 cacgccgggc gatacgctcg atgccctggt ggtgatgaac ccggcggcgc ttaagacgaa
     1921 cctcaccgac ctgcggaaag ggggcgtgct gatcgcgaac gccgacaact tcgagaagaa
     1981 ggattgggag atggcgggtt atgacgagaa cccgctcgat gcggaggttc tggagcacac
     2041 gtatcaactc tacaaagtgc cgatgaccaa gatcgtccgc gaggcgctca aggacatggg
     2101 gatgagccag aaggaagtcg atcggtgcaa gaacttcttt gcgctgggcc tggtctattg
     2161 gctgtatggc cgcgacatgg atcccacgct gcggttcctc aacgccaagt tcgccaccaa
     2221 gcccgaaatc gtggagtcca acacgcgagc cctcaaagcc ggctggaact acggcgaaac
     2281 aaccgatgcc tttgcttcga cttacaaggt cgagaaggcc aagctgccgc ccggcacgta
     2341 caagaacatc atgggcaata cggccctggc ttggggtctg gtgaccgcag cccacctgag
     2401 cggcaaagag ctgttcctcg gcagctatcc gatcacgccg gccagcgaca tcctccacga
     2461 gctcgcgaaa ttcaagaatt tcggcgtccg cacgtttcag gccgaggacg aaattgccgc
     2521 catctgct
//
LOCUS       DASUGB010001195         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1664296, whole genome shotgun sequence.
ACCESSION   DASUGB010001195 DASUGB010000000
VERSION     DASUGB010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664296"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..520
                     /locus_tag="VFV87_22640"
     CDS             161..520
                     /locus_tag="VFV87_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106643.1"
                     /translation="MRAAAGGVLLGLGLLVVWSIGFPEAQAQRPASSLVSQSSAAQDR
                     AAGGDLIVLSYDSGGTQQITVVDPRSRSLAVYQVDRVTGGLSLKSVRNVTWDLQIEDF
                     NSNSPTPREIRALTDQR"
     gene            578..1918
                     /locus_tag="VFV87_22645"
     CDS             578..1918
                     /locus_tag="VFV87_22645"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5106644.1"
                     /translation="MAGKFVELNDAAKMIGVSPEELVEMRSRGEIFGYRDGASWKFKL
                     EEVERVIGERGSRIGTGESAILSANDDEFESLISGLSSKILAEKAQEESESVLVSEEE
                     LGLSATGQSTIIGKGKQPVEVAGDSDLKLAEEAPSKPASGTGSDKLLEAPGSKLNLGG
                     ESDVLGGSDLKVSGGSGTGDMPALAPKPGSTGDLQLGEKLSLGEEDDLELGSSDSALD
                     EEIHPKKKGSGTGSGIGSDVTLGAGDSGINLSPTDSGLSLDEEPLDLVPGGSAVESLE
                     LPEDDEVISLEQASADPDQATQLKADQDFQLSPGTMEAVEDESDSGSQVIALEDSESF
                     DQDAATMLKSEPGGPLAADAFQPMPGDSLAMGSSPGAMSMGGGQPVYVQVPVVEAPYS
                     IWNVLSLMAITLMLALAAMMMVDVMLNMWSFSGSSSVTTGIMDMFTSTFSLDNR"
     gene            1956..2252
                     /locus_tag="VFV87_22650"
     CDS             1956..2252
                     /locus_tag="VFV87_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106645.1"
                     /translation="MDIKVWGRRLACPFLLLLGVAGCASGPNEQLMSCQKDKEQLLAT
                     ISQQREANRAMHEQVASLETRLDQAEKELARAASGDTRISTRPVEVAPLPWRKP"
     gene            2523..>3549
                     /locus_tag="VFV87_22655"
     CDS             2523..>3549
                     /locus_tag="VFV87_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106646.1"
                     /translation="MRRACLAALVLLAAGCGQREQITSYTVKKPELIDPTHAAAPTDA
                     KPQQILGAIVVLDESGWFFKVTGDPAQVEPQRDTFLSLVKSIRFSNGPDPRPNWDLPE
                     GWRELPGDQFRFATLQMPTDQGSLDLAISMLSRSVEADEFVLVNVNRWRGQVGLSDVD
                     QAELADSSNKFQVDGHDCTFVNLVGESSGGGMGRAPFAPFAGGASAAPGSSPTEPVRP
                     KSAATSAGDELVFEAPKGWTPGKTTEFRRAAFSVTDGDNKVEITVIPLGAGSGSLLEN
                     VNRWRGQVGLKAAQEVAGVKKIETLGIEGDYVELQGPDQSMLAVMAPVGDRVWYIKLL
                     GDPELAKR"
BASE COUNT          678 a         1119 c         1135 g          617 t
ORIGIN      
        1 cagccccttt ttggcgggcg atggccggcg gggaataccc ttgctggcat aacccgaatc
       61 gttgacgtaa cccaaaggct tgaaacggga tttggggctt ttagaataga gttctagtcc
      121 cggtttccgt ttcaggctgc tattcgcgcg ggaggatccg atgcgagccg cggcaggagg
      181 tgtgttgctc gggctgggat tgctggtggt ctggagcatc ggatttcccg aggcccaggc
      241 ccagcggccc gctagctcgt tggtttcgca gtcgtccgcg gcccaggacc gagcggccgg
      301 cggcgatttg atcgtcctga gttacgattc cggcggcacg cagcagatca ccgtcgtcga
      361 tccgcgcagc cgctcgctgg ccgtgtatca ggtcgatcga gtgacgggcg gcttgtcgct
      421 gaagagcgtt cgcaacgtga cctgggacct gcaaatcgag gatttcaatt caaatagtcc
      481 gacgccgcgc gagattcgcg cgttaacgga ccagcgctga ccaacgtagc cgtctcgctc
      541 cgcgggacga ggctcacggc agccagggga gcaccacatg gccggcaagt ttgtcgaact
      601 caacgacgcc gcaaagatga tcggcgtttc gcccgaagaa ctggtcgaga tgcgctcgcg
      661 gggcgaaatc ttcggctatc gcgacggagc gagttggaaa ttcaagctgg aggaagtgga
      721 gcgcgtgatc ggcgagcgcg gcagccgcat cggcacaggc gaatcggcga tcctcagcgc
      781 caacgacgac gaattcgagt cgctcatcag tggcctctcg tcgaagatcc tcgccgagaa
      841 ggcccaggaa gaatcggagt cggtgctggt cagcgaggaa gagctggggc tttccgccac
      901 cggccagagc accatcatcg gcaaaggaaa gcagcccgtc gaagtggccg gcgacagcga
      961 cctgaagctc gccgaagaag cgccctccaa gccggcttct ggaaccggca gcgataagct
     1021 gctggaagcg cccggcagca aactgaatct cggcggcgaa tcggacgtgc tcggcggttc
     1081 tgacctgaag gtcagcggtg gcagcggcac gggcgacatg cctgcccttg cccccaaacc
     1141 cggatccacg ggcgatttgc agctcggtga aaaattgagc ctgggcgaag aagacgacct
     1201 ggaactcggc agcagcgaca gtgccctgga cgaagaaatc catccgaaaa agaaaggcag
     1261 cggcaccggc agcggcatcg gaagcgacgt cacgctcggc gccggcgaca gcggaattaa
     1321 tctttcgccc accgacagcg gcctctcgct cgacgaagag ccgctcgacc tggttcccgg
     1381 cggctccgcg gtcgaatcgc tggaactgcc cgaggacgac gaggtaatct cgctcgaaca
     1441 ggcgtctgcc gatcccgacc aggcgacaca gctcaaagcc gaccaggatt tccagctctc
     1501 gcccggcaca atggaagcgg tggaggacga atcggacagc ggctcgcagg tcatcgcctt
     1561 ggaggactcc gagtcgttcg accaggatgc cgcgacgatg ctcaagtccg aacccggcgg
     1621 accgctggcg gcggatgcct tccagccgat gcctggcgac tcactggcca tgggctccag
     1681 ccccggcgca atgagcatgg gcggtggcca gccggtttat gtgcaggtgc cggtcgttga
     1741 ggcgccctac tcgatctgga atgtgctcag tttgatggct atcacactga tgctcgcgct
     1801 ggccgcgatg atgatggtcg atgtgatgct taacatgtgg tcattcagcg gctcgtcgag
     1861 cgtcaccacc gggatcatgg acatgttcac cagtacgttc agcctggaca accgctagat
     1921 tacgggccga cgagtgattc gaagggagtc gaatcatgga catcaaagtg tggggtaggc
     1981 gtctcgcctg cccattcctt ttgctgctcg gggtcgccgg ctgcgcgtcc ggtcccaacg
     2041 agcagctcat gagctgccag aaagacaagg agcagctcct ggcaactatc agccagcagc
     2101 gcgaagcaaa ccgcgctatg cacgagcagg tcgcctcgct cgaaacgcgg ctcgaccagg
     2161 cggagaagga actcgcccgc gccgcaagcg gcgacacgag aatctctacc cgaccggtgg
     2221 aagttgcgcc cttgccctgg cgcaaaccgt aggctatctc gctccgcgag tagcagacga
     2281 ctctccatac gcgggggtct ttgcgcgcat ccttcgattt ccaccattcg agatcgcaac
     2341 cctatccgcc tgccgaccag ccgcgctata atgagatact gccgtccgcg aagccgcttc
     2401 ggcgcgctgg cggcggccct ccctcctgga taggccggtt gtctgcccgc ccctgccggc
     2461 tgttcgactt gcctcgctcc tctcccgcga gacagcgacc gatgttattg cccggattga
     2521 ctttgcgacg cgcatgcttg gcggctctcg tgctgttggc agccggctgc gggcagcggg
     2581 agcaaatcac cagctacacc gtcaaaaagc ctgagctgat cgatccgacg cacgcggccg
     2641 cccccacgga tgcgaagccc cagcaaatcc tgggggccat cgtggtcctg gacgagtccg
     2701 gctggttctt taaggtcact ggcgacccgg cccaagtcga gccgcagcgc gatacgttcc
     2761 tcagcctggt caagtcgatt cgcttctcga acggccccga tccgcggccg aattgggact
     2821 tgccagaagg ctggcgtgag ctgcccggcg atcagtttcg cttcgcgacg ttgcaaatgc
     2881 cgaccgacca gggctcgctc gacctggcaa tcagcatgct ttccagatcc gttgaggccg
     2941 atgaattcgt actggttaac gtgaaccgct ggcgcggcca ggtcgggctg tcggacgtcg
     3001 atcaggccga gctggccgat tcgagcaaca aattccaggt ggacggccac gattgcacgt
     3061 tcgtcaatct tgtcggcgag tcctcgggtg gcggcatggg ccgtgctccc ttcgccccct
     3121 tcgcgggcgg cgcgagcgca gcccctggct cttcgccgac cgagcccgtc cgtcccaagt
     3181 ccgccgcaac ttccgcgggc gacgagctgg tcttcgaggc tccgaagggt tggactccgg
     3241 gcaagaccac cgaattccgc cgggccgcat tcagcgtgac cgatggcgac aacaaagtcg
     3301 aaatcaccgt tattccgctc ggcgccggct ccggctcgct gcttgagaac gtcaatcgct
     3361 ggcgtggcca ggtcggactg aaggccgccc aggaagtcgc cggcgtcaag aagattgaaa
     3421 cgctcggcat cgaaggcgac tatgtcgagc tgcaaggccc cgatcaatcg atgctcgccg
     3481 tgatggcccc cgtcggcgac cgcgtctggt acatcaagct gctcggcgac cccgagctgg
     3541 cgaaacgcg
//
LOCUS       DASUGB010001196         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1802471, whole genome shotgun sequence.
ACCESSION   DASUGB010001196 DASUGB010000000
VERSION     DASUGB010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802471"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFV87_22660"
     CDS             complement(<1..350)
                     /locus_tag="VFV87_22660"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5106647.1"
                     /translation="MPRTARVAPGGYVFHVLNRGNDRRTLFEADGDYLAFLRVLNETA
                     ERIPLRILAWCLMPNHWHLVLWPKEDGQLGDFMQRLTTTHVRRWHLYRHSVGCGHLYQ
                     GTYKSFPVEQDDHY"
     gene            complement(527..1513)
                     /locus_tag="VFV87_22665"
     CDS             complement(527..1513)
                     /locus_tag="VFV87_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEX5106648.1"
                     /translation="MLHLVLLAALHVPGQVELTDAKPVPRMQVLPLPRGEASIEREGQ
                     EISRYYFDRDLFRPFLYPLIGPSGKSLTRMGHPRDPNGHSHHNSVWISHHDVGGVGFW
                     NDAASSKGRIVQQRVLRYEDADDEALIEVVNAWKDDEGRGRTLLEETRTMRFIPGERR
                     QWLLVLDVILSAPKEPVTLGKTPFGLIGVRMAKTIGVHDGGGAIRNSEGGVNEAGVFW
                     KPARWCDYSGPIAREGDRDLRTRPEIPDSAAGITLLDHPINPNHPTVFHVRDDGWMGA
                     SLTFDAPRTIEPGTPLKLRYGLWVHSGVPAAERINAQFDEFAKIGDPAPAAK"
     gene            complement(1544..2038)
                     /gene="greA"
                     /locus_tag="VFV87_22670"
     CDS             complement(1544..2038)
                     /gene="greA"
                     /locus_tag="VFV87_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5106649.1"
                     /translation="MDEHYPMTRAGFAKLKAEAERLETIEMPAIAEKIAAARAEGDLS
                     ENAEYHAQREAQGMLQAKINLIKTKLSKATIIDTSTLPRDQVCFGATVVVKDADLGDE
                     EEYTLVGSGEEDYNQGRILVTSPLGQGLLGKKIGETAEIPAPKGSYELEVVAIKFDFL
                     EGDA"
     gene            complement(2220..2867)
                     /locus_tag="VFV87_22675"
     CDS             complement(2220..2867)
                     /locus_tag="VFV87_22675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106650.1"
                     /translation="MLRKSLVGLLVLTAVVLACSSSLLAQGKGKGLSKALSKAGTSGG
                     ASAGLNRAGQNASRVGGSLNRANGLGRSGALGGNALGGNAVGGPAPSGKGFGSARGLQ
                     KPPAETSTMAGADAATRHQRQLLIEQTNRDRRIAQAQHLREIAARNGDAELAANADRL
                     EAYANDHYLDRVGHQSRFGVTDPDLPPNPLPGDPPAPPSDPLAQPGDPTTGQPTP"
BASE COUNT          591 a         1113 c         1081 g          650 t
ORIGIN      
        1 taatagtgat cgtcctgctc gaccgggaac gacttgtacg tcccctgata caagtgtccg
       61 cagccgacgc tgtggcgata gagatgccag cggcggacgt gggtggtggt cagccgctgc
      121 atgaagtcgc cgagctggcc atcctccttg ggccacagca ccaggtgcca gtggttgggc
      181 atcaggcacc aggcgagaat ccgcaggggg atgcgctcgg ccgtctcgtt cagcacgcgc
      241 aagaacgcca gataatcgcc gtcggcctcg aagagcgtgc gacggtcgtt gccccggttg
      301 agcacgtgga agacatatcc gcccggcgcg acgcgtgcag ttctcggcat gcccgccagg
      361 atacgccacc gcctcgccac cgtcaataat tggacttatc cccttttatc accccctttt
      421 atcaccgttt tatcgcccct ggagaggatg atctcgccgc cggaaagtcg cctgtcaacg
      481 aaattctgaa gatccagcac cagagttctt gcccaccacg acggggctac ttggcggctg
      541 gtgctggatc gccgattttg gcgaactcgt cgaactgagc gttgatccgc tcggcggccg
      601 gcacgcccga gtggacccac aagccatagc gtagcttcag cggcgtgccg ggctcgatcg
      661 tgcgtggggc gtcgaacgtg agcgaagccc ccatccagcc gtcgtcgcgg acgtgaaata
      721 cggtgggatg attgggattg atcggatggt cgagcagcgt gatccccgcc gcgctgtcgg
      781 gaatctcagg tcgggtgcgc aagtcccggt cgccttcgcg ggcgatcggc ccggaataat
      841 cgcaccagcg ggccggcttc cagaaaacgc cggcttcgtt gacgccccct tcgctgttgc
      901 ggatggcgcc gccgccgtcg tggacgccaa tggtcttggc catccgcacg ccaatcagcc
      961 caaacggggt tttgccgagt gtcaccggct ctttgggcgc ggagagaatc acgtccaaca
     1021 ccaacagcca ctgccgccgt tcgccgggaa tgaaacgcat cgtccgcgtc tcttccagca
     1081 gggtgcggcc acggccttcg tcgtctttcc aggcattgac cacctcgatc agcgcctcgt
     1141 cgtccgcgtc ttcatagcgc agcacgcgct gctggacgat tcgccccttg ctcgaagcgg
     1201 catcgttcca aaagccgacg ccgcccacgt cgtggtgcga aatccacacg ctgttgtggt
     1261 gactatggcc gttgggatcg cgcgggtggc ccatccgcgt gagcgatttg ccgctgggac
     1321 cgatgagcgg atagaggaag ggccgaaaga gatcccggtc gaagtaatac cgcgaaatct
     1381 cctgcccctc gcgctcgatg ctcgcctcgc ctcgcggcag cggcagcacc tgcatccgcg
     1441 gaacaggttt agcgtctgtc agctcaacct ggcccggaac gtgcaaagca gccagcagca
     1501 cgagatggag catgttgatg tccctcagcg aaccagggct ggttcaagca tcgccttcca
     1561 ggaaatcgaa tttgatcgcc acgacttcca actcgtacga acctttgggc gccggaattt
     1621 cggccgtttc gccaatcttc ttgcccagca ggccttggcc gagggggctg gtgaccagaa
     1681 tgcgtccctg gttgtagtcc tcctcgccgg agccgacgag cgtgtattct tcctcatcgc
     1741 cgagatcggc atccttgacg accaccgtgg cgccgaagca aacctgatcg cgcggaagcg
     1801 tcgaggtgtc gatgatcgtc gccttcgaga gcttggtctt aatcaggttg atcttcgctt
     1861 gcaacattcc ttgcgcttcg cgttgggcgt ggtattccgc gttctcggac aggtcgcctt
     1921 cggcccgcgc agcggcaatc ttttccgcga tcgcgggcat ctcgatcgtc tccagccgct
     1981 cggcttcggc tttgagcttg gcgaaaccgg cacgcgtcat cggatagtgt tcgtccatgg
     2041 ctcgttggag tgtgagggcg ttgtttgtgc agacaaaaaa cgcgacctcc gaggggaggc
     2101 cgcgtgtaaa atgatcagca ctcaattggc caaaagaccg gatcagcgct atcggcgcat
     2161 tgtgcgacat ccgctgccgg atgtaaaggt gaagaacgcc ggcgctgatt ggcggacggt
     2221 tacggcgtcg gctggccggt tgtggggtcg ccgggctgcg caagcgggtc ggacggaggg
     2281 gcgggcggat cgccgggaag cggattgggc ggcagatcgg ggtcggtcac gccaaaccgc
     2341 gactgatgtc cgacccggtc gagatagtgg tcgttggcgt acgcttccag ccgatcggcg
     2401 ttggcggcca gttcggcgtc gccattgcga gcggcaattt cccgcagatg ctgggcctgc
     2461 gcgatccgcc gatcgcgatt cgtttgctcg atcaggagtt gccgctggtg ccgcgtagcc
     2521 gcatccgcgc cggccatggt gctggtttcg gcgggtggct tctgcagccc acgtgcggag
     2581 ccgaaccctt tgccgctggg agctgggccg cccactgcgt ttccgcccag agcgttgcct
     2641 cccaaagcgc cggaccggcc gaggccatta gcccggttca ggctgccccc cacgcgcgaa
     2701 gcattctggc cggcgcgatt gaggccggcg ctggccccgc cgctggtgcc ggccttagag
     2761 agggccttgc tcagcccctt gcccttgccc tgtgccaaca gcgaagaact gcaggccaga
     2821 acgacagcag tcagaaccag cagccccacc aaagacttcc gcagcatgaa gcaatccttt
     2881 gcgttgcgag gtatcgcggc cggcgcgatg ctcatccgca gcaccgcact tgctgtcatc
     2941 ggcaaagtgc ggtccgcccc ttgaatggcg atgtgctagc aagctggcgg acggcggcag
     3001 aggccgatcc gcctgaatcg cgaaatccgc cgaacaggtc aaggccggct tgtgttgacg
     3061 ggttctcagg tcgccggtac cctcgctccc ccgattacga actctcacgg ccagccgatc
     3121 gtgccagcat ttcagccgcg gcgccattgc gaactccgat ctatatcgca ctgcctgggt
     3181 gccgggctgg tcgccctgat ggcgattgct gccgattcct cgcgcgcaca agctctgggc
     3241 cctcctcccg gaccgcaatc gtcgcccctc gccctgcccg ctccgcccgc agatccagaa
     3301 agcgtcatcc gcttgccccc ggtcatgtcc gcggaagagg aactcctgca attggagcag
     3361 gaagcagcgc gaatcatcgc gctcgatgag gcccggttcg cgcccgacga acatgccgcc
     3421 aaaacggccc tctcg
//
LOCUS       DASUGB010001197         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_133059, whole genome shotgun sequence.
ACCESSION   DASUGB010001197 DASUGB010000000
VERSION     DASUGB010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133059"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1851)
                     /locus_tag="VFV87_22680"
     CDS             complement(313..1851)
                     /locus_tag="VFV87_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106651.1"
                     /translation="MDKRITLIAGLTALFAVGSVHAATCPVPAPSEVTTAINDETGHT
                     FFLYASPGITWDQAQACVSALPQQSGVPAHLATITSSSENQWIVDVLLLNNVGAPRMA
                     LTQVWGGGSQGAGAAEPREGWRWVNNEGPIPVPGSTVTLGYSNWSPAPNLEPNNQGGN
                     EEQLTLGRYSADLGRWNDEGSAPGSIGGFIVEYDTPRTSTAGCTPSGTSTVCTTVEGQ
                     SLTFPPGTFTNNSSITFTAFEFTDPRVDTSGRCVGIGGVRGPLTLFDDPAFNLPGGAS
                     GKLVIPSHLCGSPKFVVVRAVGTNITIPRGVVVTESETSIILPDNIYDCNNPIPAGGS
                     PQQQDVGAWQSLDAREMWESHPTVPDSRFDDGTAAELTTACASTLIKTRAKSNFVSGL
                     HVDFGPAFAFGSAAEYFQVIAWTQYKISLVRQSVVDARAKGRISNANGQAMLSLLDAA
                     AAALTANNPVGAKKKIEDFLKKVNSSTYSTSLTDPFNYNGDHLARGENIRFTLIHKAI
                     PFMP"
     gene            complement(2077..>3333)
                     /locus_tag="VFV87_22685"
     CDS             complement(2077..>3333)
                     /locus_tag="VFV87_22685"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5106652.1"
                     /translation="ASLLDSALQKRLDLYGADSLEVAQSAEKLGGVQTLTAEYGKAET
                     NYRNALNLRQKLLGPEHVDIARSQYRLADLLFREGKYEAAEPLFRDALAMQRKLLGGN
                     SLEVAKSLEGLALNHYKQGKYDEALPPMRLAVELQRGLHKEPHTDLAEALSNLGTVLS
                     DKGEYQEAEKLYREGLQMKRVLLGDTHPEIAASLINIAYVLTDEKKYDQAAAMYREAI
                     AMQRKLLGDDHPDIAAALNNLAFVLHDKGDAKGARRMMQESVDMYRRTVGPEHPSTAR
                     GMANVAMWQMEAGDYASAEPLLRETLQLQRKLLGNEHTDVAGALTLLATLLVETRRFE
                     EARAAAAEARAICLPALGAADWRTAAASSTEGAALLGLGQTAQGAKLLLEGLAALQAD
                     VGALPFNVRNAARWAADYQLKRPGAT"
BASE COUNT          560 a         1049 c         1050 g          674 t
ORIGIN      
        1 cgtgacagca aggcggtcat tcaaatctcc ttcgtggaat ctgcagccaa actaagggca
       61 agcacgagcc tgcgcactgc ctctggtcgc ggcaaaccgg catcctggct caattggatc
      121 gtggtcagga agagtgttgc aagccgagat tatgagctga tccacaagac gtgctcccgc
      181 aggatgcgac cgcggggatc aactagggca cttgcgtcag actgcggcgc gctgaggggt
      241 cagccaatac gctgacccct gggaacgaga cgaagcccgt gctttgagcc acgggcttcg
      301 tctggcgtca ggtcagggca tgaacggaat ggccttgtgg atcagcgtaa accggatgtt
      361 ctcgccacga gccaggtgat cgccgttgta gttgaatgga tccgtcaggc tcgtcgagta
      421 agtcgacgaa ttcaccttct tcaggaaatc ttcaatcttc tttttagcac cgactggatt
      481 atttgcggtg agggcggcgg ccgcggcatc cagcagcgaa agcatggcct gcccattcgc
      541 attcgaaatc ctgcctttgg cgcgggcatc gaccaccgac tgccgcacca gcgagatctt
      601 gtactgggtc cacgcgataa cctggaaata ctcagccgcg ctgccaaagg cgaaggcagg
      661 tccaaagtcg acgtgcagcc cggacacgaa gttggacttg gcacgggtct tgatcaaggt
      721 gctggcgcaa gcggtcgtga gttcggccgc cgtaccgtca tcaaagcgtg agtcggggac
      781 cgtcggatgg gattcccaca tctccctcgc gtccagggac tgccacgcgc cgacgtcctg
      841 ctgctgcggg ctcccgccgg ccgggatcgg gttgttacag tcgtagatat tgtcgggcag
      901 gataatgctc gtctcgctct cggtgaccac gacgcccctg ggaatggtga tatttgtccc
      961 caccgccctc acgaccacga atttggggga accgcacagg tgcgacggga tcaccagctt
     1021 gccgctcgcg ccgccaggga ggttgaaagc cggatcatcg aacagggtca gcggcccgcg
     1081 aactccgccg atgccgacac accttccgct tgtgtctact cgcgggtccg tgaattcaaa
     1141 cgccgtgaac gtgatactgc tattgttggt aaacgtcccc ggggggaagg tcagcgactg
     1201 gccttcgacg gtcgtgcaga ccgtggacgt gccactgggg gtgcaaccag cagtcgaagt
     1261 gcgaggcgtg tcgtactcga caatgaagcc gccgatcgaa cccggcgccg agccttcgtc
     1321 attccagcgg ccaaggtctg cgctgtaccg gccaagcgtt agctgctcct cgttgccgcc
     1381 ttgattgttg ggctccaggt tgggcgccgg ggaccagttg ctgtagccga gagttaccgt
     1441 cgatcccggc acgggaatcg gcccttcgtt gttcacccaa cgccagcctt cgcggggttc
     1501 tgcggcgcca gctccctggg atccaccgcc ccagacctga gtcagcgcca tccgcggcgc
     1561 gccgacgtta ttcagcagca acacatcaac gatccactgg ttctcgctgg acgacgtgat
     1621 ggtcgccagg tgcgccggca cccctgattg ctgcgggagc gcgctgacgc aggcttgggc
     1681 ctggtcccat gtgattcctg gggacgcgta cagaaagaag gtgtgaccgg tttcatcgtt
     1741 gatggccgta gtcacttcgg acggagcggg aacggggcag gtggcagcgt gcaccgatcc
     1801 cacggcaaac agcgccgtga gacctgcaat cagggtgatt ctcttatcca tgtccagctc
     1861 cccctcagtc agcaaggcca atggccgttg cgatgtcttg cgttttgttt accgcgggtc
     1921 tcggaatgcc ggacccccgc gacgtgcggt cctctggggc cgaatcgacc ttgtgtaagg
     1981 tgaccgcaat ttgtggaccc tatcacaatt tcggttgcca gatgactgga acggcttgcg
     2041 tagccggcag attatgtcag tcgggggcct tggcgtttac gtcgcgccgg ggcgcttgag
     2101 ctggtagtcg gccgcccatc gcgcggcatt tctgacgtta aagggtagcg ccccgacgtc
     2161 ggcctgcagc gccgccagcc cttcgagcaa cagcttggca ccctgcgcag tctggcccag
     2221 gcccagcagg gcggcgccct cggtactgct ggcggccgcg gtgcgccagt cggcagcgcc
     2281 cagcgccggc aggcagatcg cccgtgcttc cgctgcggcg gcccgcgcct cctcgaagcg
     2341 tctggtctcg accagcaggg tggccagcaa ggtcagcgca ccggcgacgt cggtgtgctc
     2401 gttaccgagc agttttctct gcaactgcag cgtctcgcgc agcagtggtt cggccgacgc
     2461 gtagtcgccc gcttccatct gccacatcgc cacattggcc atgccgcgtg ccgtcgacgg
     2521 atgctcgggc cccaccgtgc gccggtacat gtcgaccgac tcctgcatca tcctgcgggc
     2581 gcccttggcg tcgcccttgt cgtgcaaaac gaaagccagg ttgttcaggg ccgctgctat
     2641 gtccgggtga tcgtcgccga gcagcttgcg ctgcatggca atggcctcgc ggtacatggc
     2701 cgccgcttgg tcatactttt tttcgtccgt aaggacatag gcgatattga tgaggctcgc
     2761 cgcgatttcg gggtgcgtgt cgccgagcag cacgcgcttc atctgcaaac cttcccggta
     2821 gagtttttcg gcctcctggt actcaccctt gtcgctcagc accgtgccga gattgctcag
     2881 cgcctcggcc aggtccgtgt gcggctcttt atgcaatccc ctctgcagct ctacggcgag
     2941 gcgcatcggt ggcagggctt cgtcgtattt tccctgcttg tagtggttca acgccaggcc
     3001 ttccaggctc ttggccacct cgaggctgtt gccgccgagc agcttgcgct gcatcgccag
     3061 tgcatctcga aacaagggct cggctgcctc gtacttgcct tcgcggaaga gcaggtcggc
     3121 cagccggtac tggctgcgcg cgatgtcgac gtgttcgggt ccgagcagtt tctggcgcaa
     3181 gttgagcgcg ttgcggtaat tcgtctccgc cttgccgtac tcggcggtca gcgtctgcac
     3241 cccgccgagc ttctcggccg actgcgccac ctcgagactg tccgcgccgt agagatcgag
     3301 gcgcttctgc agtgcactgt cgagcagcga cgc
//
LOCUS       DASUGB010001198         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1522846, whole genome shotgun sequence.
ACCESSION   DASUGB010001198 DASUGB010000000
VERSION     DASUGB010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522846"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFV87_22690"
     CDS             complement(<1..192)
                     /locus_tag="VFV87_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5106653.1"
                     /translation="MLRSFASVNVTGEGVASRRGFLKTLGVGAAAGTAVSLSWRDMLL
                     AKAPELAQSGKRMILLWMDG"
     gene            complement(305..613)
                     /locus_tag="VFV87_22695"
     CDS             complement(305..613)
                     /locus_tag="VFV87_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106654.1"
                     /translation="MQIPVIVEPTQCGQFRAQPLYALEAVGEGQTSEAALANLREALE
                     REFQKGKQIVFMDFPSEEYNPWLAMAGSLKDDPLYDEWRAAMEENRDQSDREAGIERD
                     "
     gene            complement(645..1082)
                     /locus_tag="VFV87_22700"
     CDS             complement(645..1082)
                     /locus_tag="VFV87_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106655.1"
                     /translation="MSTILVLMVLFGGFFVWLRDYVHNRPIAWVPYSAEALARKRSEG
                     RVVMVLVDANWHPAVTYTRVFAVETPGVRKFLRSHGIIAMEADWSEPSPDVNELLASL
                     NEQSVPIVAVFPAAEDATPITLRDMATEDQVLAALQEASGKPP"
     gene            complement(1324..1659)
                     /locus_tag="VFV87_22705"
     CDS             complement(1324..1659)
                     /locus_tag="VFV87_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106656.1"
                     /translation="MKGPFIRGSHRGITRIAPGPWEEHILIRGHRYRVVKEFKDEDGD
                     LHPIGEDWVFVGSLFSPHDELMLVVVRTRPVFEWEFELEWRSSERDNVVEHFGDYVEL
                     VSRDSRRIR"
     gene            complement(1668..2729)
                     /locus_tag="VFV87_22710"
     CDS             complement(1668..2729)
                     /locus_tag="VFV87_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5106657.1"
                     /translation="MSPIARRSFLSGSVAALSAAALSPRLFAQQAPMLEDPERASALL
                     IKPETQKAIDRGLAWLASRQNDDGSFGGSGYSRNVAVVSLSGLAFMAGGNTPGRGEYG
                     EHVARCLSYVLAASDDSGFICEPMYTSHGPMYDHGFATLFLAEVYGMSMDSEVRDRLA
                     KAVRLIVSTQNIDGGWRYQPKASEADISVTICQVMALRAAKNAGIYVPNETIDRTIDY
                     VKRSQNADGGFMYMITGGPSRFPRSAAGIVALYSAGIYEGDEITKGLEYLMTNMPAAE
                     DFSRDTHAMYGHYYAVQAMWQAGGDYWHKWYPAVHDVLLRQQQEDGSWMDLICPEYGT
                     AMACIILQMPNNYLPIFQR"
     gene            complement(2804..3460)
                     /locus_tag="VFV87_22715"
     CDS             complement(2804..3460)
                     /locus_tag="VFV87_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106658.1"
                     /translation="MRSIAIIAAVIASILAPASLPAQESKPPKPSSLDQQLLDDLDRD
                     LLKDLPGSAKPAPAKGNEDVTPAVQSDNPLAQLAERMRAVEARIANKDTSPETQKLQK
                     QILAELAALMEQAKKQGGGSNKPGDGQGKGSDQPGTGSGNPTAGPVRDSTGRIEKGTA
                     EAAETADVKDLLRRMWGHLPDKVRDQMQASLSEQFLPKYERLIEEYYKRLAEERPAGP
                     "
     gene            complement(3457..>3964)
                     /locus_tag="VFV87_22720"
     CDS             complement(3457..>3964)
                     /locus_tag="VFV87_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106659.1"
                     /translation="DAAPAFAFALEGIVERMRQAAAGLTRGETAQPTQQLEQAALARL
                     EQVLAALKPNDPAAGEDMPQPNQQENPMGQPPPGNLAGAMAELKLLKLLQEEINRRTA
                     ELEDLRVKNNQLLPDEEGELASLAREQGRLADMVYNLIEATAPRPEDNPDALPDPLPK
                     EAEEKEKP"
BASE COUNT          680 a         1247 c         1204 g          833 t
ORIGIN      
        1 gccgtccatc cagagtaaaa tcatccgctt gccgctctgg gccagctccg gcgccttcgc
       61 cagcaacatg tcccgccagc tcaagctgac cgccgttccc gccgcggccc cgacgcccag
      121 cgtcttcaag aacccacggc gcgaagcgac gccttccccg gtgacattga cggaggcaaa
      181 cgaacggagc atggcggggt ctcctggaga ggcttgatcc tagcccatca ttctagccgt
      241 tccccatcga cctttgcacc cactagttgg accaatcttc aacttgggga agaccggaag
      301 accgctagtc ccgctcgatg cctgcctctc ggtcagattg atcccgattc tcctccatcg
      361 cagcccgcca ttcgtcatac agcggatcgt cctttagcga gccggccatc gccagccagg
      421 ggttgtattc ctcacttgga aaatccatga acacgatctg cttgcccttc tggaattccc
      481 tttccaatgc ctcgcgcaga ttcgcgagcg ccgcttcgct ggtttggccc tcgccgactg
      541 cttccagcgc gtagagcggc tgagcccgaa actgaccgca ttgcgtgggt tcgacgatga
      601 cggggatttg catctctgta gctccgtatc cattatacgc cgtgctacgg cggcttgccg
      661 ctcgcttctt gtaatgccgc aaggacctga tcctctgtag ccatgtcgcg cagcgtaatc
      721 ggagtggcat cctctgcggc tggaaaaact gcgactatcg gaacggattg ctcattgagc
      781 gatgcgagca attcgttcac atcgggcgac ggctcggacc aatctgcttc cattgcgata
      841 atgccgtggc tgcgcagaaa cttccgcacc cccggagtct cgacggcgaa gacgcgagtg
      901 taagtgacag ccgggtgcca attggcatcg accagtacca tgacgactcg cccttccgac
      961 cgcttccggg ccagtgcctc cgcggagtag gggacccagg cgatcggccg attgtggaca
     1021 tagtcgcgga gccagacaaa gaatccgccg aataaaacca tcaatacgag aatggttgac
     1081 aaccggaatt gccaagggcg aaatgatcgc ttcttcgcgg ttggcggttg gggccgaaga
     1141 gacgggagct ccaagaaaaa tgtgtcgcgg tctttgatca ttgggcgatg gccccagaca
     1201 tcacgacttt ggatcttccg atgctctcgg tcgggtagct gcaacgcgta gacgcggaat
     1261 tgaccgcatt gacgatgatg ggattttgta tttcccgctc cgcggcgatt actctgcacc
     1321 ccactagcga attcgtcgag aatctctgct caccagctcc acgtaatcgc cgaagtgctc
     1381 gacgacattg tcacgttcgc tggatcgcca ctccaactcg aattcccact cgaaaaccgg
     1441 ccttgtgcgt acgacaacca acattagttc gtcatgtggc gagaataaac tacctacgaa
     1501 tacccaatct tcaccaatcg ggtgcaaatc accatcttcg tccttgaact ccttgacgac
     1561 gcgataacga tgcccgcgaa ttagaatgtg ctcttcccag gggcccggcg caatacgagt
     1621 aatgccgcga tgacttcccc gaatgaatgg gcctttcatt attcaggcta ccgctggaag
     1681 atcggcaggt agttattcgg catctgcagg atgatgcagg ccatggcggt gccgtattcg
     1741 gggcagatca ggtccatcca ggagccgtct tcttgctgct ggcggaggag cacgtcgtgg
     1801 accgcggggt accacttgtg ccagtagtcg ccgccggctt gccacatcgc ctgcaccgcg
     1861 tagtagtggc cgtacatcgc gtgcgtgtcg cggctgaagt cctcggccgc cggcatgttc
     1921 gtcatcaggt attcgagccc cttggtgatc tcgtcccctt cgtagatgcc ggcgctatac
     1981 agggcgacga tgccggcggc cgaccgcggg aagcggctgg ggccgccggt gatcatgtac
     2041 atgaagccgc cgtcggcgtt ctggctgcgc ttgacgtagt cgatcgtgcg gtcgatcgtt
     2101 tcgttgggga cgtagatgcc ggcgttcttc gccgcgcgga gggccatcac ctggcagatc
     2161 gtgaccgaga tgtccgcctc actggccttg ggctgatagc gccagccgcc atcgatgttc
     2221 tgcgtactga cgatcaggcg gaccgccttg gccaggcggt cgcggacctc gctgtccatc
     2281 gacatgccat agacttccgc aaggaaaagc gtcgcgaagc cgtggtcgta catcggcccg
     2341 tggctggtgt acatcggctc gcagatgaag ccgctgtcgt cgctggcggc caggacgtaa
     2401 ctgaggcagc gggcgacatg ctcgccatat tcgccgcggc ccggcgtatt cccgcccgcc
     2461 atgaacgcga ggcccgaaag gctgaccacc gccacattgc ggctgtagcc gctgccgccg
     2521 aagctgccgt catcgttctg ccgcgatgcc agccaggcga ggccgcggtc gatcgctttc
     2581 tgtgtttccg gcttgatgag caatgccgag gcacgctcgg gatcttcgag catcggcgcc
     2641 tgttgggcga agagccgggg cgagagtgcg gccgcgctca aagcggcgac ggaaccggag
     2701 aggaacgagc gacgagcgat gggggacata gtgcctccgt ggggtaggcg tctcgcctgc
     2761 ccgtcatcat tcaactcggg caggcgagac gcccgcccca cgattatggc ccagcgggcc
     2821 gctcctccgc cagcctttta tagtattcct cgatcagccg ttcgtatttg ggcaggaact
     2881 gctcgctgag cgatgcttgc atctggtcgc ggaccttgtc gggcagatgg ccccacattc
     2941 gtcgcaagag gtccttcacg tcggctgttt cggccgcctc ggctgtgccc ttctcgatgc
     3001 ggcccgtgct atcacgcacc gggccggcgg tcggattgcc gctgccggtt ccgggctgat
     3061 cagagccctt tccctggcca tcgcctggct tattagagcc gccgccctgc ttcttggctt
     3121 gctccatcag ggcggccaat tcggcgagaa tctgcttctg cagcttctgc gtttcgggcg
     3181 aggtgtcctt gttcgcgatg cgagcttcga ccgcccgcat tcgctcggcg agctgcgcga
     3241 gcggattatc ggactgaacc gcgggtgtta cgtcctcgtt accctttgcc ggggccggtt
     3301 tcgcgctccc cggcaggtcc ttcagtagat cgcggtcgag atcgtcgagg agctgctggt
     3361 cgagagagct cggcttcggc ggcttcgact cctgggcagg tagcgacgca ggcgcaagga
     3421 ttgacgcaat gacagccgcg attatcgcaa tcgatctcat ggcttctcct tctcttccgc
     3481 ctctttcggc agcgggtccg gcagggcatc tggattgtcc tccggacgcg gcgcggtcgc
     3541 ttcgatcaaa ttatagacca tgtcggccag ccggccttgt tctcgggcga gggaggcgag
     3601 ttccccttcc tcgtcgggca aaagctgatt gttcttgacg cgcaagtctt ccaactccgc
     3661 ggtgcggcgg ttgatctctt cctgcagcag cttgagtagc ttcagctcgg ccattgcgcc
     3721 ggccagattg ccgggcggcg gctggcccat cggattctct tgctggttgg gctgcggcat
     3781 gtcctcgcct gccgcgggat cgttgggctt gagcgcggcg agcacttgtt ccagccgggc
     3841 tagagcagcc tgctcaagct gctgggtggg ctgggccgtt tcgccgcgcg tcaggccggc
     3901 cgccgcctgc cgcatgcgtt caacgatccc ttccagcgcg aaggcgaacg ccggtgcggc
     3961 atcg
//
LOCUS       DASUGB010001199         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1245844, whole genome shotgun sequence.
ACCESSION   DASUGB010001199 DASUGB010000000
VERSION     DASUGB010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245844"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFV87_22725"
     CDS             <1..523
                     /locus_tag="VFV87_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5106660.1"
                     /translation="TSVQDVDYTKLRERLLADKQVLDWTGPRPTPPIDPKKLPGIVQD
                     DEQAEKRGEWSKSNSIGGYIGTQYLHDNNDHKGELSVTFKLPLSKPGTYEVRLAYTAN
                     PNRATNVPVTIHHATGESVVTVNQKKDPADKIFQSLGKYAFDKEAIVAISNAKTDGYV
                     IVDAVQILPVDAK"
     gene            complement(539..988)
                     /locus_tag="VFV87_22730"
     CDS             complement(539..988)
                     /locus_tag="VFV87_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5106661.1"
                     /translation="MTKPTPEPINPTRVRELALGVIAADRFPMLATIDGDSPRLRPVS
                     PVKTDGFTIYVANLRMYHKTEEIAANPKVELCFMDEGHNQVRLAGAAEIVTERPLLEE
                     IWNANPLMRQYLGSLDNPQLVVYRIRPSRVRYMQEWALEYYEVPLET"
     gene            complement(993..>2043)
                     /locus_tag="VFV87_22735"
     CDS             complement(993..>2043)
                     /locus_tag="VFV87_22735"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5106662.1"
                     /translation="APPKAAPQAAAEAAEEAADQQLPDGRPWHRRWLAATPSWLVSLV
                     IHLALIVLLALLVVPAPKQALGTLLEAVTSELEEVELLEPMKMEMVELQTPEMATTSV
                     TAVEVTSTSLADAADLSSVAANLSPGDVEVPASSEIGDLFVGGGGKAMGNVLPSGADK
                     SAQFFGVKASGRRFVFIVDSSNSMRGGKFDAAKEELMYAIRRLSKEQAFYIIFFDQNA
                     ERMRLPPSTEPELLPVPAVNENINRAEKWVGTVANELRTDPYDAVKFAIDMVPDAIYL
                     LTDGKFTDRGLTERYLKNNNIIDDPLEGRRPKVVIHTICFWQKDGELALQAIAKDYGG
                     TYRFVPPARGMMKKK"
BASE COUNT          387 a          706 c          582 g          368 t
ORIGIN      
        1 caccagcgtc caggacgtcg attacacaaa gctccgcgag cggctgctgg ccgataagca
       61 agtcctcgac tggacgggcc cgcggcccac gccccccatc gaccccaaga agctgccggg
      121 aatcgtccaa gacgacgagc aggccgaaaa gcggggcgag tggagcaaga gcaactcgat
      181 cggcggctac atcggcacgc aatacctgca cgacaataac gaccacaaag gcgagctcag
      241 cgtcacgttc aagctaccgc tttccaaacc cggcacgtac gaagtccgcc tcgcctacac
      301 ggccaatccc aaccgcgcga cgaacgtgcc ggtcacgatc caccacgcga cgggcgaaag
      361 cgttgtgacc gttaatcaga aaaaagaccc cgccgacaag atcttccaat cgctcggcaa
      421 gtacgccttc gacaaggaag ccatcgtcgc catcagcaac gccaaaaccg acggctacgt
      481 gatcgtcgat gcggtgcaga tcctgccggt agatgcgaag tagccaaccg tgagcgcatc
      541 acgtttcgag cggcacctcg taatactcca gcgcccactc ctgcatatac cgcacgcggc
      601 tgggccggat gcggtagacg acgagctgcg ggttgtcgag cgagccgagg tattgccgca
      661 tcagcgggtt ggcgttccaa atctcttcca agagcggccg ctccgtgaca atttcggccg
      721 cgccggccag ccgcacctgg ttgtgccctt cgtccatgaa gcacagctcg accttgggat
      781 tcgccgcgat ctcctcggtc ttgtgataca tccgcaagtt cgcgacatag atcgtgaacc
      841 cgtccgtctt caccggcgag accggcctta gccgcggcga atcgccgtcg atcgtggcca
      901 gcatcggaaa gcggtcggcc gcaatcacgc ccagagccaa ctcgcggacc cgcgtcgggt
      961 taatcggttc aggcgttggt ttggtcacgg cctcacttct tcttcatcat tccccgcgcc
     1021 ggcggcacga agcgatacgt gcccccgtaa tccttggcga tggcttgcag cgccagttcg
     1081 ccgtctttct gccagaagca gatcgtgtgg atcaccacct tcggccggcg gccctcgagc
     1141 ggatcgtcga tgatgttgtt gttcttcaga taccgttcgg tcaggccgcg gtcggtgaac
     1201 ttgccgtcgg taagcaggta gatcgcatca gggaccatgt cgatcgcgaa cttcaccgcg
     1261 tcgtaagggt ccgtccgcag ctcgttggcg acggtgccca cccatttctc cgcccggttg
     1321 atgttctcgt tcaccgccgg cacgggcaac agctctggct cggtgctcgg cggcagacgc
     1381 atccgttcgg cattctggtc gaagaaaatg atataaaacg cctgctcctt gctgagccgg
     1441 cgaatggcgt acatcaactc ttccttggcg gcgtcgaact tgccgccccg catgctgttg
     1501 gagctatcga cgataaacac gaaccgccgg ccgctcgcct tcacgccgaa gaactgggcc
     1561 gatttgtccg ctccgctcgg cagcacgttc cccattgcct tgccgccgcc gcccacgaac
     1621 aggtcgccga tctcgctact cgcaggaact tcaacgtcgc ccggcgaaag attcgccgcc
     1681 accgagctca gatcggcggc atccgccaga ctcgtgctcg tcacttccac tgccgtgacg
     1741 ctggtcgtcg ccatctcggg cgtctgcaat tcgaccatct ccatcttcat cggctccagc
     1801 agctcgacct cctccagctc gctggtaacc gcttccagca gcgtgcccag cgcctgcttc
     1861 ggcgcgggga cgaccagcag cgccagcagc acgatgagcg ccaaatggat gaccaggctc
     1921 accagccagc tcggcgtcgc cgccagccag cggcgatgcc acggacggcc gtccggtaat
     1981 tgctgatccg cagcctcttc cgccgcttcg gctgcggctt gcggcgctgc cttgggcggc
     2041 gct
//
LOCUS       DASUGB010001200         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1107841, whole genome shotgun sequence.
ACCESSION   DASUGB010001200 DASUGB010000000
VERSION     DASUGB010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107841"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFV87_22740"
     CDS             complement(<1..1300)
                     /locus_tag="VFV87_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106663.1"
                     /translation="MTPPAATPHSAFRTPQFGLSRRSCLRSLAGASLILPGMLSQLLA
                     EDSARGDSRGVPLDPLAPQEPHFEPKAKRVIFVFSSGGVSHMDTFDHKPELFKADGKI
                     TGAGGGLSREQKPLVKPRWEFKPGGECGTMVSDLFPHLRDQMDDIALIRSMKGQENEH
                     YQATLAMHTGSFFFTRPSLGAWLSYGLGTMNQNLPSFIVIAPNLPYAGGQVWGNDFLP
                     AYHQGTRVVPGAEPIPNLKRRTQQEGLQELELDLAEALNKKHLAAIGNDSELAARIRS
                     FETAFHMQAAAPEALDIRQESDDTLALYGLKRGDTESFAWQCLVARRLAERGVRFIEL
                     IDRGASNNWDAHSDMAQHEPLAKAIDQPLAGLIKDLKQRGMLDETLVVWTTEFGRTPG
                     QDGATGRGHHSACFSSWLAGGGVKGGIAYGETDDIGATVAS"
     gene            1377..2123
                     /locus_tag="VFV87_22745"
     CDS             1377..2123
                     /locus_tag="VFV87_22745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5106664.1"
                     /translation="MATSNADRSAPATGIPPLGVIGTLLVFGGGAILLFAATHGLIPL
                     LASRTAAEPVLLWFIAGGLGTLVPLVVVGIVILRGETSRPLVVLWRDRLRFRRMNAGD
                     WLWSFTALLLVGLFAAGMLAALRLAWPGIQLHPPFLKMDPLTPGRYWILAAWLPFFVL
                     NILGEEFLWRGVVLPRQEAAFGKWTWLANGGGWLLFHLAFGPTILATLFPIVFILPFV
                     VQRRKNSWIGVVIHGGLNGPGFLAVAFGFA"
     gene            complement(2194..>3509)
                     /locus_tag="VFV87_22750"
     CDS             complement(2194..>3509)
                     /locus_tag="VFV87_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106665.1"
                     /translation="SNPALAKPIGFYTWNDDLKKCWSFMRFFQNPLEENDPIAGEIVK
                     ALEGDPALAADCQQAAQFYGKLTNPLTRLTFADAVGTDLSSDEALESLRMSKGVTTPG
                     LSLFPPSSSRETELYQRIFPLGVPEAANLMKELVTRIRSGEIDLSPRENSGWYDHQVY
                     ALETMLLPERGEEHNKLLLTKPYKKRMLEAFQALITKRRETHSRGLPAAEATAALPPD
                     EFLPRLRIEPCPSYYLRTARSYAFLHEFLTSSLGPETLQTLHGLRQRGEQEADLLTEL
                     ESQRDLFYGLYLISCEDIGHKPALADGEPADPDAYYSAAEEWLGQLEDEPDLAEDTRV
                     AVPIYVDPLQGKMRLWVTLGVRLTQLDTKFVKAPRIKPASGEGDWQEVEAWKLADAPF
                     LISVDEFAEVEVPTLSPPNREELRKLCDEHKTKEKIVEALAAGNW"
BASE COUNT          564 a         1083 c         1151 g          711 t
ORIGIN      
        1 cactggcgac cgtcgcgcca atgtcgtcgg tctcgccata agcgatgccc cctttgacgc
       61 cgccacccgc cagccacgag ctgaagcacg ccgagtgatg cccgcggccg gtcgcgccat
      121 cctgccccgg cgtgcggccg aactcggtcg tccagacgac gagcgtttcg tcgagcatgc
      181 cccgctgctt gaggtccttg atgaggcccg ccagcggttg gtcgatcgct ttggccagcg
      241 gctcgtgctg ggccatgtcg ctgtgggcat cccagttgtt gctggccccg cggtcgatca
      301 gctcgatgaa ccgcacgccc cgctccgcca gccggcgagc cacgagacac tgccaggcaa
      361 agctctcggt gtcgccgcgc ttcaagccat acagcgccag cgtgtcatcg gactcctggc
      421 ggatatcgag cgcctccggc gcagcagctt gcatgtggaa agccgtctcg aacgagcgaa
      481 tgcgggcggc caattccgaa tcgttgccga tggccgccag gtgctttttg ttgagcgcct
      541 cggccagatc gagctccaac tcctgcaggc cttcctgctg cgtgcggcgc ttgagattgg
      601 gaattggctc ggcgccgggg acgacgcgcg tgccctggtg atacgcgggg aggaaatcgt
      661 tcccccacac ctgtccgccg gcatagggca gattcggcgc gatgacgatg aagctcggca
      721 gattctggtt catcgtcccc aggccatagc tcagccacgc gccgaggctc ggccgcgtga
      781 agaagaacga accggtgtgc atcgccagcg tcgcctggta gtgctcgttc tcctgcccct
      841 tcatcgagcg gatcaaggcg atgtcgtcca tctggtcccg caggtgcggg aacaggtcgc
      901 tgaccatcgt tccgcactcg ccgccgggtt tgaattccca ccgcggcttg accagcggct
      961 tttgctctcg ggacaggcca cctcccgcgc cggtgatctt gccgtctgcc ttgaatagct
     1021 cgggcttgtg gtcgaacgtg tccatgtgcg atacgccgcc cgacgagaag acgaagatca
     1081 cgcgcttggc cttcggctca aagtgcggct cttgtggcgc gagtggatcg agcggcacgc
     1141 cgcgcgaatc gcctctcgcc gagtcttccg ccagaagctg cgacaacatt cccggcaaaa
     1201 tcagcgacgc gcccgcgagc gaacgcaggc acgagcggcg ggagaggccg aactgcggag
     1261 tgcggaatgc ggagtgggga gtggcagcgg gcggcgtcat gttaggattg ccaacttcgg
     1321 gcgggcagaa tcaacgctgg tttcagaata tccggcgaat tgcaggaaag cgatacgtgg
     1381 ctacttcgaa cgcggaccgc agcgcacccg cgactggcat tccgccgttg ggagtaatcg
     1441 gcacgctgct ggttttcggc ggcggggcga tcttgttatt cgcagcgacg cacggcctga
     1501 tcccgctgct ggccagccgc acggctgccg agcccgtgct cctgtggttt attgccggcg
     1561 gcctcggaac gttggtgccg ctggttgtcg tgggaatcgt catattgcga ggcgagacgt
     1621 cacgtccgct ggttgtgctg tggcgcgacc ggcttcgctt tcgccggatg aacgcgggag
     1681 actggctgtg gagtttcacg gcgttgttgc tggtcggtct gttcgcggca ggcatgttag
     1741 cggcactgcg gctggcctgg cctggaatcc aactgcaccc gccattcctc aagatggatc
     1801 cgctcacgcc ggggcgttac tggattctgg cagcctggct gccgttcttt gtcctcaata
     1861 tcctgggcga agagtttctc tggcggggtg tcgtgctgcc ccgtcaggaa gcagctttcg
     1921 gaaagtggac gtggctggcc aatggcggcg gctggctgct atttcacctg gccttcgggc
     1981 ccacgattct ggcgacgctc tttccgatcg tgttcatcct gccgttcgtt gtgcagcggc
     2041 gaaagaactc gtggatcggc gtcgtgattc acggtggact gaacgggccc gggtttctgg
     2101 cggtggcctt tgggttcgcc tgagtgtagg gtgggctttg cccaccagat tgcaattcga
     2161 cagccgagcg ggcagaggtg ggcacagccc accctaccag ttgcccgctg ccaaagcttc
     2221 gacgatcttc tccttcgtct tgtgctcgtc gcagagtttg cggagctcct cgcgattggg
     2281 aggcgagagc gtggggactt cgacttcggc gaattcatcg acggaaatca ggaacggcgc
     2341 gtcggccagt ttccaggctt cgacctcctg ccaatcgcct tcgccgctcg ccggcttgat
     2401 gcggggggct ttgacgaact tcgtatcgag ctgcgtcagc cgcacgccca gcgttaccca
     2461 cagccgcatt ttgccttgca gcggatcgac gtagatcggc acggcgacgc gcgtgtcctc
     2521 ggcgagatcc ggctcgtcct cgagctggcc cagccactcc tcggcagccg agtagtaggc
     2581 gtcgggatcg gccggctcgc cgtcggccag cgccggcttg tggccgatgt cttcgcagct
     2641 aatgaggtac aagccataga ataggtctcg ctgagattcc aattcggtca gcagatcggc
     2701 ttcctgctcg ccgcgttgtc gcaagccgtg cagtgtttgg agcgtttccg gcccgagcga
     2761 gctggtgagg aattcgtgca ggaatgcata cgaacgggcc gttcgcaggt aatacgacgg
     2821 acagggctca attcgcaggc ggggcaagaa ttcatcgggt ggaagcgctg cagtagcttc
     2881 cgcagccggc aacccacgcg aatgcgtctc gcggcgcttg gtgatcagcg cctgaaacgc
     2941 ttcgagcatc cgcttcttgt acggcttggt gagcagcagc ttgttgtgtt cttcgccgcg
     3001 ctcgggcaag agcatcgttt ccagggcata gacctgatgg tcgtaccagc cgctgttttc
     3061 gcgaggcgaa agatcgatct cgcccgagcg gatgcgcgtc accagctctt tcatcaggtt
     3121 cgccgcttcg ggcactccga gcgggaagat ccgctgatag agctccgtct cgcgcgagct
     3181 cgagggcggg aagagcgata ggccgggcgt tgtgacgcct ttgctcatcc gtagcgattc
     3241 gagcgcctcg tccgacgaca ggtctgtgcc gaccgcatcg gcaaacgtca gccgcgtcag
     3301 cgggttggtc agcttgccgt agaactgggc tgcctgctgg caatccgcag cgagcgccgg
     3361 atcgccttcc agcgccttca cgatctcgcc cgcgatgggg tcgttctctt ccagtggatt
     3421 ctggaagaac cgcatgaagg accagcactt tttcaagtcg tcattccacg tgtaaaagcc
     3481 gatcggcttg gcgagggcgg gattggaca
//
LOCUS       DASUGB010001201         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_2080818, whole genome shotgun sequence.
ACCESSION   DASUGB010001201 DASUGB010000000
VERSION     DASUGB010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080818"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFV87_22755"
     CDS             complement(<1..290)
                     /locus_tag="VFV87_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5106666.1"
                     /translation="MTIRVYNTLTKQKEPFETVRLGKVGIYLCGPTVYKESHIGHMVG
                     PVIFDTIKRFLTYSGYEVTWVVNITDVDDKLIGQMHERGLPMSEIADEMTID"
     gene            complement(329..802)
                     /gene="ispF"
                     /locus_tag="VFV87_22760"
     CDS             complement(329..802)
                     /gene="ispF"
                     /locus_tag="VFV87_22760"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5106667.1"
                     /translation="MRVGIGHDTHRLDQGGPLLLGGIEIPHDRHLVGHSDADVLLHAI
                     TDAILGAAALGDIGELFPDTDEANRGRDSAEMLKAAAAKVRSAGYEIGNLDCIVFAQR
                     PKLTPHKEAIRRRIAEILQIEPGCIGVKAKTGEGIGEIGREEALMAQCVALLESN"
     gene            complement(939..1502)
                     /locus_tag="VFV87_22765"
     CDS             complement(939..1502)
                     /locus_tag="VFV87_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106668.1"
                     /translation="MNLLFEQPLVVVVLGIVLGLAAGTAWTSTGRKEWLIALAAIVVL
                     TIAGLVIERGIVTDREAIEATLAQIARELQRNDQQAVLKHIATGATRIRQEAEAELPN
                     YRFDECRVTKVHNVDIDDSAEPRSAIVEFNVMATGSFSHGGFEVSEQNVYRWIQLQMV
                     REKDGQWRVQRYEHDDALRAFQSRPGQ"
     gene            complement(1499..1861)
                     /locus_tag="VFV87_22770"
     CDS             complement(1499..1861)
                     /locus_tag="VFV87_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106669.1"
                     /translation="MAPRPPRRPITDSPWFWAYLFAVAALVALALVGTKYGPRQAQIE
                     REFQGRQRAAQNLNQQQPNVEMSTSERTLVTLQPLFVVLAGITIVAWIVFWRRRLHGG
                     SGDQSSAQETFSAEHRSA"
     gene            complement(1871..2689)
                     /gene="pstB"
                     /locus_tag="VFV87_22775"
     CDS             complement(1871..2689)
                     /gene="pstB"
                     /locus_tag="VFV87_22775"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5106670.1"
                     /translation="MTRAALTVASRSGEPFVPEAPLKAEVCALSFFYGDRQALKKVNL
                     KIPERSVTALIGPSGCGKSTLLRCFNRMHDLYAGNRYEGEIVLQPRGINILSPQVDPM
                     EMRLRTGMVFQKPNPFPKSIFENVAYGLRLRFVNQRAVLEEKVQAALEAAALWDEVKD
                     RLHQSALKLSGGQQQRLCVARALATDPEILLLDEPTSALDPIASSRIEDMIFALKEQV
                     TILIVTHNMEQAARVADFTGFMYLGELVEHGATSQVFQTPKNPLTEKYLTGSFG"
     gene            complement(2752..>3027)
                     /locus_tag="VFV87_22780"
     CDS             complement(2752..>3027)
                     /locus_tag="VFV87_22780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HEX5106671.1"
                     /translation="ETAPLIVIGAAGYITRLPPPPISTAAPFLSFKWLNSSFTVLPMQ
                     MYLWTADADPGLQSRAATTGLVLIVLTLSVNAAAIYLRYRVRRKLKW"
BASE COUNT          560 a          969 c          909 g          589 t
ORIGIN      
        1 tagtcgattg tcatctcgtc ggcgatctcg ctcatcggga ggccccgctc gtgcatctgg
       61 ccgatcagct tgtcatcgac gtcggtgatg ttgacgaccc acgtcacctc atagccgctg
      121 taggtcagga accgcttgat ggtgtcgaaa atgaccggcc cgaccatgtg gccgatgtgg
      181 ctttccttat agaccgtcgg cccgcacagg tagatgccga ccttccccag ccgcaccgtc
      241 tcgaagggct ctttttgttt cgtcagggtg ttatagacgc ggatcgtcat tggtcagttg
      301 tcgctcgtca gttgtcagtt gtcagtggtc agttggattc gagcagggcc acgcactggg
      361 ccatgagcgc ttcctcgcgc ccaatctcgc cgatcccttc acccgtcttg gccttgacgc
      421 cgatgcatcc tggctcgatc tggaggatct ccgcaatccg gcggcggatc gcttccttgt
      481 gcggcgtgag ctttggccgc tgggcgaaga cgatgcagtc cagattgccg atctcatagc
      541 ccgcggagcg aaccttggct gccgcagctt tgagcatctc ggctgaatcg cggccgcggt
      601 tcgcttcgtc cgtgtcggga aacaattcgc caatgtcgcc cagtgccgcc gctcccagga
      661 tcgcgtccgt gatcgcgtgc agcagcacgt cggcatcgct gtggccgacg agatgccggt
      721 cgtgcggaat ctcgattcca ccgagcaaga gcggccctcc ctggtccagc cggtgcgtat
      781 cgtgtccgat gcccacgcgc atcacaggcg gaagtccttc tcccagcatc cacaacggcc
      841 gccatcgccg cccagccggg cgaagagcag caaagcgagc cgattatatc ggcacgcccc
      901 cgccggaaca atgcggacgc ggcgccttcg ccctcgcgct actgtcccgg gcggctttga
      961 aacgcccgca acgcgtcgtc gtgctcgtac ctctgtaccc gccattgccc gtccttttcg
     1021 cggaccattt gcagctgaat ccagcgatag acattctgct cggacacctc gaacccgcca
     1081 tggctgaacg agccggtggc catgacgttg aattccacga tggccgaacg aggttcggcg
     1141 gagtcgtcaa tatcgacgtt gtggactttc gtcacgcggc actcgtcgaa ccgatagttg
     1201 ggcagctcgg cttcggcctc ctgccggatt ctcgtggcgc cggtggcgat atgctttaac
     1261 acggcctgct ggtcgttgcg ctgcagttcg cgagcgatct gggccagcgt cgcctcgata
     1321 gcctcgcggt cggtgacgat cccgcgctcg ataaccaggc cggcaatcgt cagcacgaca
     1381 attgccgcca gcgcgatgag ccattccttg cggccggtcg aggtccaggc cgtcccggca
     1441 gccaggccca ggacgatgcc caggacgaca accactagcg gctgttcgaa aagcagattc
     1501 atgccgagcg atgctccgcg gagaaagtct cttgggctga ggattggtca ccggaaccac
     1561 cgtgcagacg cctgcgccag aaaacgatcc aggctacgat cgtgattcct gccagcacga
     1621 cgaacagcgg ctgcagcgtg accagagtcc gctcggacgt ggacatttca acgttcggct
     1681 gctgctggtt caggttctgc gcggcccgct ggcgtccctg aaactcgcgc tcgatctgag
     1741 cctgccgcgg gccgtatttc gttcccacca gggccagcgc caccagcgcc gccacggcga
     1801 agagataggc ccaaaaccaa ggcgaatcgg tgatcggacg gcgtggcggt cgaggagcca
     1861 tcccgtcatt ctaaccaaag gagcccgtga ggtacttttc cgtcagcggg ttcttggggg
     1921 tctggaagac ctgcgaagtc gccccgtgct cgaccagttc gcccagatac atgaatcccg
     1981 tgaaatcggc cacgcgagcc gcctgctcca tattatgcgt gacgatcagg atcgtcacct
     2041 gctccttcag ggcgaagatc atgtcctcga tccgcgagga agcgatcgga tcgagagcag
     2101 aagtcggctc gtcgagcagc aggatctccg gatcggtggc cagagcccgg gccacgcaca
     2161 atcgctgctg ctgtccgccg gaaagcttca gggccgattg gtgcagccga tccttcacct
     2221 cgtcccacaa tgccgccgcc tccagcgccg cctgcacctt ctcttccaga accgcacgct
     2281 gattcacgaa ccgcagccgc aggccgtacg ccacgttctc aaagatcgac ttgggaaatg
     2341 gattcggctt ctgaaaaacc atccccgtcc gcagccgcat ttccatcgga tcaacctgcg
     2401 ggctcaagat attgatacct ctcggctgca gcacgatctc gccttcgtag cggttgcccg
     2461 cgtacaggtc gtgcatccgg ttgaagcacc gcaaaagcgt gctcttgccg cagccggacg
     2521 ggccgatcag cgccgtcacg ctgcgctcgg ggatcttcag gttgactttc ttcagggctt
     2581 gtcgatcacc gtaaaagaaa ctgagcgcgc agacttccgc tttgagcggc gcttccggca
     2641 cgaagggctc gccggaacga ctggcgactg tcaatgcggc gcgcgtcatc gtggagaatc
     2701 gaggttatca ctttggccag ccggaatgac gagccaggac gcgcattgag tttaccactt
     2761 gagctttctc cggacacgat accgcagata aatggcggcg gcgtttacgg agagggtaag
     2821 cacaatgagg accagccccg tcgtggcggc acgcgactgc aatccagggt cggcgtcggc
     2881 cgtccacagg tacatctgca tgggcagcac ggtaaacgag gaattcagcc acttgaagct
     2941 caaaaacggc gctgctgtgc tgatgggggg tggcggcaac cgcgtgatgt acccagcggc
     3001 gccaatgaca atgagtggag ccgtttc
//
LOCUS       DASUGB010001202         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_830107, whole genome shotgun sequence.
ACCESSION   DASUGB010001202 DASUGB010000000
VERSION     DASUGB010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830107"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..349)
                     /locus_tag="VFV87_22785"
     CDS             complement(8..349)
                     /locus_tag="VFV87_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106672.1"
                     /translation="MTPGKTIRVGALASARSGDKGNHANICVVANDQAAYGVLCSRLT
                     AVRVQTHFQELGVARVERYELPQVLALNFVLYDALAGGASRSLRTDTQGKLLATSLLD
                     LTFHANAVADE"
     gene            complement(346..1731)
                     /locus_tag="VFV87_22790"
     CDS             complement(346..1731)
                     /locus_tag="VFV87_22790"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX5106673.1"
                     /translation="MRTIRIANGAGFLGDWIDAPRRLVERAEVDYLTIEHLAELTMSI
                     LARLREKDPAAGYAEDFLHVVGTLMPALKSQPRLKIVANSGGVNPRACAQAVAKLLVA
                     AGLGEALIGCVTGDDFLPQIDSLAAHEELRNLDTGESLESAIRKPQSAIVSANAYLGA
                     RPIADALAKDARIVITGRVADASLTVGPAMHEFGWQWDDWNKLAAASVAGHLIECGAQ
                     VTGGYSVNWQDYELADIGYPIAEVSEDGSTVITKPPGTGGAVTRRTVVEQLVYEIGDP
                     QHYLTPDVDCDFTSISVQEIGADRVQVSGATGRPATETYKASLAYRDGWMASGTLLVY
                     GPDCREKAEACARIILERCRLAGYELARSNVELLGYGGGVPGAWFWRKYQSPGELVLR
                     VAAADPRREAIECFARQFAPLITSGPAGLAGYAAGRPQVRPVYAYWPGLIAKSQVADS
                     VEVRSARGWQA"
     gene            2040..>2363
                     /locus_tag="VFV87_22795"
     CDS             2040..>2363
                     /locus_tag="VFV87_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5106674.1"
                     /translation="MNTRQRAEGGFTLVEMLVVIAIIGVLTAMLIPAVQFARERARQT
                     TCLNHLRQLGVASQLHNTQHQRFPNAGLFDESGSTWNRGPKVYINNATKLENATATGR
                     ALEGWG"
BASE COUNT          425 a          799 c          733 g          406 t
ORIGIN      
        1 ggttctttca ctcgtccgcc accgcgttgg cgtgaaacgt caggtccaat agcgacgtgg
       61 ccaggagctt gccctgcgta tcggttcgca agctgcggct ggcgccgccg gccagggcgt
      121 cgtagagaac gaaattgagg gccagcacct ggggaagctc gtaccgctcg acgcgcgcca
      181 cgcccagctc ctggaaatga gtctgcacgc gcaccgccgt caagcggctg cagagcactc
      241 cataagcggc ctggtcattc gcgacgacgc agatgttggc gtggttcccc ttgtcgccgc
      301 tgcgagccga ggccagagcg cccacgcgga tggtttttcc gggcgtcatg cttgccaccc
      361 ccgggcacta cggacctcga cgctgtctgc gacttgcgat ttggcaatga gccccggcca
      421 gtaggcatag acggggcgca cttgcggccg gccggctgcg tatcccgcca aaccggccgg
      481 tccgctggtg atcagcgggg cgaattggcg ggcgaaacac tcaatcgcct cgcgccgcgg
      541 atcggcagcc gcgacccgga gtacaagttc ccccggcgat tggtatttcc gccagaacca
      601 ggcacccggc acgccgccgc cataacccag cagctcgacg ttgctccgcg ccagctcgta
      661 gcccgccagc cggcaccgct cgagaataat ccgggcgcag gcctcggcct tctcgcggca
      721 atcggggcca tagaccaaca gcgtgccgct agccatccag ccatcgcggt aggcgagcga
      781 agctttgtac gtctcagtcg ccggacggcc cgttgcgccg ctgacctgca cgcgatctgc
      841 gccgatttcc tgcacgctga tgctggtgaa gtcgcaatcg acatcgggcg tcaggtaatg
      901 ctgcggatcg ccgatttcat agacgagctg ttcgacaacc gttcgccgcg tcactgcgcc
      961 gcccgtgccc ggcggcttgg taatgaccgt gctgccgtcc tccgacacct cggcaattgg
     1021 atagccaatg tcggccagct cgtagtcctg ccaattcaca ctgtatccgc ccgtgacctg
     1081 cgcgccgcat tcaatcaagt gccccgcgac gctggccgca gccagcttgt tccagtcgtc
     1141 ccactgccag ccgaattcgt gcatagccgg gccgaccgtc agcgaggcat cggccacgcg
     1201 gcccgtgatg acgatgcggg cgtctttggc cagggcgtct gcgatcggcc tggcgccgag
     1261 atacgcattg gccgagacga ttgcggattg cggcttgcgg attgcggatt ctaaactctc
     1321 gcccgtgtcg aggttccgca gctcctcgtg cgcggccagc gagtcgatct gcgggagaaa
     1381 gtcgtcgccc gtgacgcagc cgattaaagc ctcgccaagc cctgccgcga ccagcaactt
     1441 ggcgaccgcc tgggcgcagg cccgtgggtt cacgccgccc gagttggcga cgattttcag
     1501 cctaggctgg ctcttcaagg cgggcatgag cgtgccgacg acatgcagaa aatcctcggc
     1561 ataaccggcc gccgggtcct tttcccgcaa gcgggctagg atcgacatcg tcaactcggc
     1621 caggtgctcg atcgtcaaat aatcgacctc ggcccgctcg accagccggc ggggcgcatc
     1681 gatccaatcg cctaaaaatc cggctccgtt ggcgatgcga atcgtccgca tggagtggct
     1741 cgcctgcccg attaacccta ctgttttaga tcaatcgccc gcacagattc taaggggcgt
     1801 cagcctcttt cggccacggc gctcgcactc gggcggcgtc cgccggaaga aaacgcccaa
     1861 aaaccgccgg atcgtacttg ctccctgcca caggccactc gataatccct tgtggcgtac
     1921 tgcctgcaag cggaagaggc ctcttgtcgc cggcagcacg cagccttgct ccctgcgccg
     1981 ccctattcgg ggccgtccgg cgctgggatg agcacgagaa acggaacaag gagccgggca
     2041 tgaatactcg acagcgggcc gagggcggat tcacgctggt ggaaatgttg gtggtgattg
     2101 cgatcatcgg cgttctgacg gccatgctga ttcccgccgt gcagttcgcc cgcgagcgag
     2161 cgcggcagac gacctgcctg aaccacctgc ggcaactggg agtcgcctcg caactgcaca
     2221 acacgcagca ccagcgattc ccaaacgcgg ggctgttcga cgaatcgggc agcacatgga
     2281 accgtggccc caaggtctat atcaacaacg ccaccaagct cgaaaatgcg accgcgacgg
     2341 gccgagccct ggaaggatgg ggt
//
LOCUS       DASUGB010001203         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1523441, whole genome shotgun sequence.
ACCESSION   DASUGB010001203 DASUGB010000000
VERSION     DASUGB010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523441"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFV87_22800"
     CDS             complement(<1..726)
                     /locus_tag="VFV87_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX5106675.1"
                     /translation="MGFQLHQPFPPAGDQPQAIARLTEGLRAGKRSQVLLGVTGSGKT
                     YTMANVIAAVQKPTLVISHNKTLAAQLYSEFREFFPKNAVHYFVSYYDYYQPEAYIPQ
                     RDIYIEKDASINEEIDRLRLLATSALVSRRDVVVVASVSCIYGLGSPKDYKSMMVPLR
                     VGESIDRDEMLMRLVDIQYDRNDVSFERSKFRVRGDVVELWPAYEEVGYRIELFGDEV
                     ETLAVIDPLTGAVLEKKEEMYVYP"
     gene            868..1962
                     /locus_tag="VFV87_22805"
     CDS             868..1962
                     /locus_tag="VFV87_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HEX5106676.1"
                     /translation="MRYTNVCLESLGYALPDEVVSSEEIERRLEPLYRRLRLPEGRLE
                     LMSGIRERRLWPRGMRPSEQSVNSGRRAIEAAGIDPGDIGCLIHGSVCRDYLEPATAC
                     SVHHALGLPENCLAYDVSNACLGLLNGVLQVANLIELGQIRAGLVVGTEDSRALLEST
                     IDALNRNQSLTRESVKPAFASLTIGSASAAVLLVHPDLSRTGNHLSAAAARARTSHHE
                     LCQGGLECDGTLMQTDSEKLLAEGLATGAATFADFLTASGWSRDEMHKTICHQVGGTH
                     RKLMLQSLGLPVKRDYVTYPWLGNTGSVALPVTLALALESGFVQPHEHVALLGIGSGI
                     NSIMIAASGAPKLARPHFDVAQVIPAASPS"
BASE COUNT          363 a          650 c          641 g          375 t
ORIGIN      
        1 ggggtagacg tacatctcct ctttcttttc gaggaccgcg ccggtcagcg ggtcgatcac
       61 ggcgagcgtc tccacctcgt cgccgaacag ctcgatccgg tatccgacct cctcatacgc
      121 gggccaaagt tcgaccacgt cgccgcggac gcgaaacttg ctccgctcga acgacacgtc
      181 gttgcggtcg tactggatat cgaccagccg catcagcatc tcgtcccggt cgatcgattc
      241 gcctacccgc agcggcacca tcatcgactt gtaatccttg ggcgagccca agccgtagat
      301 gcaggagacg ctggccacga cgacgacgtc ccgccggctg acgagcgcgc tggtggccag
      361 caggcgcagc cggtcgatct cttcgttgat cgaagcgtct ttctcgatgt agatgtcgcg
      421 ctgcgggata taggcttccg gctggtagta gtcgtaatag ctgacgaaat aatggacggc
      481 gtttttgggg aagaactcgc ggaactcgct gtacagctgg gccgccagcg tcttgttgtg
      541 cgagatcacc agagtcggct tctgtacggc ggcaatgacg ttcgccatcg tgtacgtctt
      601 gcccgagccg gtcaccccca agagcacctg ggaccgcttg ccggcacgca gaccctcggt
      661 caatcgggcg atcgcctgcg gctggtcgcc cgcaggagga aacggctgat ggagttggaa
      721 acccatactt ctgtttcttt agccggaagc taacagcttc cggagaatgg cgaatagagg
      781 ttcaaccggc cgttcacggg cgggccggtt tgccgacggc caatggccgc ctaggcggcc
      841 tgcgacgata cctctattct aacctgtatg cgctatacga acgtttgcct ggaatcgctc
      901 ggctacgcgc tgcccgacga agtcgtcagc agcgaggaaa tcgagcggcg gcttgagcct
      961 ctgtaccgcc ggttgcggct gcccgaaggg cggctggaac tgatgagcgg cattcgcgag
     1021 cggcggctgt ggccccgcgg catgcgtccc agcgagcaaa gcgtcaatag cggccggcga
     1081 gcaattgagg ccgccggcat tgacccgggg gacatcggct gcctgatcca cggctcggtc
     1141 tgccgcgatt atctggagcc ggccaccgcc tgcagcgttc atcatgctct cggcctgccg
     1201 gaaaactgcc tggcgtacga cgtttcgaac gcctgcctgg gcctattgaa tggcgtgctg
     1261 caggtggcga acctgatcga attgggtcag attcgtgccg gcctggtcgt cggcacggaa
     1321 gacagccgcg cgctcctcga aagcacgatc gacgccctca atcgcaatca gtcgctgacc
     1381 cgcgagagcg tcaagcccgc ctttgcctcg ctcaccatcg gctcggccag cgcggccgta
     1441 ctgctggtgc atccagactt gagccgcacc ggcaaccacc tgagcgcggc cgccgccagg
     1501 gctcgcacct cgcaccacga actgtgccaa gggggactgg agtgcgatgg gacgctgatg
     1561 cagaccgact cagagaagtt gctggccgaa ggcctggcga cgggggccgc caccttcgcc
     1621 gattttttga ccgccagcgg ctggtcgcgc gacgaaatgc acaagacaat ctgccaccag
     1681 gtcggcggca cgcatcgcaa gttgatgctg cagtcgctcg gcctgccggt caagcgcgac
     1741 tatgtcacgt atccctggct gggaaacacc ggctcggtcg cgctgccggt gacactggcc
     1801 ctggccctgg aaagcggatt tgtccagccg cacgagcacg tcgccttgct gggcattggc
     1861 agcgggatca acagcatcat gatcgcggcc agcggggcac ccaagcttgc gcggccgcac
     1921 ttcgacgtgg ctcaagttat tccggccgca tcgccctctt gatgaggtcc gcgtcgaagt
     1981 agttgatgct catgccggcg tccacgacga gcgattgcgc gttgatccc
//
LOCUS       DASUGB010001204         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1940792, whole genome shotgun sequence.
ACCESSION   DASUGB010001204 DASUGB010000000
VERSION     DASUGB010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940792"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1728)
                     /locus_tag="VFV87_22810"
     CDS             complement(145..1728)
                     /locus_tag="VFV87_22810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5106677.1"
                     /translation="MFRCRQCNPLWPVAAILLALTAASAAERPANIVLFLVDDFGWSD
                     LGCYGSEFYETPKVDRLTLEGVRFTQAYAACHVCSPTRASLMTGKYPARLKLTDWLPG
                     RREFPFQPLKNAAIRQHLPLDETTIAEALREHGYKTGHFGKWHLGEEPSGPLQHGFDV
                     QVPRWNKGWPKVGYHAPFQLEGLEDEPGDYLTDRLTDEALKFIEDNAGRPFFLYLSHF
                     AVHDPIQGRADLVAKYQAKLKRRPAGKDAAFILEGNPDAKSPLSRDKLDSLLGEDAYQ
                     GYRVLPNRTVKIKQRQDNVQFAAMVEAMDESLGRVVEKLQALGLADNTIVIFTSDNGG
                     MSAANFGRPDRVIAPAQLDAAYSTSNLPLRGGKGWLYEGGIRVPLVVKWPAQGRRGAV
                     CDVPVISTDIYPTLLQMIGAPLRPAQHADGISIAPLVKGAKTLEREAIYWHFPHYSNH
                     GMQSPGGAVRWGDYKLIEYFENSTVQLFDLRHDPGERQDLAAGQPEVAAKLKAMLHRW
                     RTEVSAEMMPANPDYRPEP"
     gene            1888..>2169
                     /locus_tag="VFV87_22815"
     CDS             1888..>2169
                     /locus_tag="VFV87_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106678.1"
                     /translation="MDETQVQQSVAETPSAVGRSESRSEKSPLPSGMEINLGDFTRTG
                     GLMKSLRLLLNDQIAVSVEEIASGVSEAGRKFKMGDIDQANQDVARLYAA"
BASE COUNT          401 a          660 c          711 g          397 t
ORIGIN      
        1 cgtttatgct aaacggcgtc gggctgggcg ctacgacgat tggatccggc cagggaagcg
       61 gcatggggct gagcatctcc ttggatcgag cgagaatcgg cggctggcag ccgccgataa
      121 gcaacattca gcaagccagc gcattcaggg ctccggccgg tagtctggat tggccggcat
      181 catctcggcc gagacctccg tccgccagcg gtggagcatg gccttcagtt ttgcggccac
      241 ttccggctgc ccggctgcga gatcctgccg ctctcccggg tcgtggcgaa gatcaaacag
      301 ctgcaccgtg ctattctcaa aatactcgat cagcttgtag tcgccccagc gaacggcccc
      361 accggggctt tgcatgccgt gattgctgta atgcgggaag tgccagtaaa tcgcctcccg
      421 ctccagcgtt ttggccccct tcacgagcgg agcgatgctg atcccatcgg cgtgctgagc
      481 gggccgcagc ggcgcgccga tcatctgcag cagggtcgga taaatgtcgg tgctgatcac
      541 cgggacgtcg caaacggcgc cccgccggcc ttgggcgggc cacttcacaa cgagggggac
      601 gcgaatgccc ccttcataga gccagccttt tccgccgcgg agcggaagat tggacgtcga
      661 ataggcggcg tcgagctgag caggagcgat gacccggtct ggccgcccaa agtttgccgc
      721 cgacatgccg ccgttgtcgg acgtgaagat tacgatcgtg ttgtcggcca ggccgagcgc
      781 ctggagtttc tccacgacgc ggcccaggct ctcatccatg gcctcgacca tcgcggcgaa
      841 ttgcacgttg tcctggcgct gtttgatctt gaccgtccgg ttgggcagga cccgataacc
      901 ctggtaggcg tcttctccga gcagcgaatc gagcttgtcc ctggagagcg gacttttggc
      961 gtcggggttc ccctccagaa tgaaggcagc gtccttcccg gcggggcgtc gcttgagctt
     1021 cgcctggtat ttcgcgacga ggtccgcgcg tccctggatc ggatcgtgga cggcgaaatg
     1081 agacaggtac aggaaaaacg gccggccggc attgtcctcg atgaacttca gggcttcgtc
     1141 ggtcaggcga tcagtcaggt agtcacccgg ttcgtcctcg agcccctcca gctgaaacgg
     1201 agcatggtag ccgaccttcg gccagccctt gttccagcgg gggacctgaa cgtcgaatcc
     1261 gtgctgcaat gggcccgatg gctcctcgcc cagatgccat ttgccgaaat ggccggtctt
     1321 gtagccgtgc tcgcggagcg cttcggcgat cgtcgtttcg tccagcggga ggtgctgccg
     1381 gatggcggcg tttttcagcg gctgaaaagg aaattcgcgg cggccgggaa gccagtccgt
     1441 cagcttgagc cgggcggggt acttgccggt catgaggctg gcccgcgttg gcgagcatac
     1501 atggcacgcc gcataggcct gagtgaaccg cacgccctct agggttagcc gatcaacttt
     1561 cggagtttcg tagaactcgc tgccgtagca gcccaggtcg gaccagccaa aatcgtccac
     1621 gagaaacagc acaatgttgg ccggccgctc ggcagccgac gcggcagtca aggccaggag
     1681 aattgccgcc accggccaca gcggattgca ttgtctgcag cggaacatga gttacccccg
     1741 ttgggatcga ggcccacatt atgcgccaat tcatcgtgag acccgagtgc cgcggccggc
     1801 tggggccgcc agaattgcgc gagcgcaccg tgctgcgtgt tcgtaacagg ctgccaccga
     1861 cgcttagccc ggcgtaatat ggagggaatg gacgaaacgc aagtgcaaca gtccgtcgcc
     1921 gaaacgccgt cagccgtcgg ccgctcggaa tcgcggagcg aaaagtcgcc gcttcccagc
     1981 ggaatggaaa tcaacctggg ggatttcacg cgaaccgggg gcctgatgaa gtcgctgcgg
     2041 ctattgctga acgaccagat cgcggtcagc gtggaagaga tcgccagcgg cgtcagcgaa
     2101 gcgggccgga aattcaagat gggggacatc gaccaggcca atcaagacgt ggcccgtttg
     2161 tatgcggcc
//
LOCUS       DASUGB010001205         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1940951, whole genome shotgun sequence.
ACCESSION   DASUGB010001205 DASUGB010000000
VERSION     DASUGB010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940951"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2033
                     /gene="uvrA"
                     /locus_tag="VFV87_22820"
     CDS             <1..>2033
                     /gene="uvrA"
                     /locus_tag="VFV87_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909204.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HEX5106679.1"
                     /translation="STVATSTEIHDYLRLLFARAGVASCHVCGQPIRQQSLQQIQDAL
                     MALPEGTKLMLLAPLVRGRKGQHAEVFEHIRKAGQVRVRVDGTVYDLEQTPPLDGRAA
                     HTIEAVVDRIVIREGSRGRLAESLALAVKQGEGVVVAAVQGAREQGTGDRVQDTEWDD
                     RLFSTLYACPNCRTSIAEIEPRTFSFNSPYGACPACDGLGYKLQFDPDLVVPDISQSI
                     AGGAVAAWRTKAATSGRGSRKMGTEDRGQETGDRRQGTGVRSQREAVAAFLESQQTSL
                     DTPLTEMRRMVREKLFAGDGKEFLGLLTLLEQEYSTATDAGRREELESFRGHVTCVAC
                     GGARLRPEALGVRIGGQNIADICRLGIAEAREFFCNLTAAIQNPKSKIQNAAGEPRPL
                     ATALEAEIAAPILHEVSARLGFLEKVGLEYLTLDRPSDTLSGGELQRVRLASGIGSGL
                     AGILYILDEPSIGLHPRDNQRLIDALRDLQAGDSTVLVVEHDEAIMRQADWLIDIGPG
                     AGSRGGSVVAEGTPAEVMAGPDTITGRYLAGALSIPIPAQRRKAAKSRSIVIEGVTTN
                     NLQNVTATFPLACLIGISGVSGSGKSSLINETLAPALVRRLGGMAPKPGPHTALRGVS
                     QIDKVIAIDQSPLGRSPRSNAATYTGALDEIRKVFAGTKEARQLGFKANRFSF"
BASE COUNT          384 a          636 c          705 g          308 t
ORIGIN      
        1 ggagcacggt cgctaccagc accgagattc acgactacct gcggctgctg tttgcccggg
       61 cgggcgtggc ctcgtgccac gtttgcggcc agccgatccg ccagcagtcg ctgcagcaga
      121 ttcaagacgc gctcatggcc ctgccggagg ggacaaagct gatgctcctc gcgcctctgg
      181 tgcgcgggcg taagggacag cacgctgaag tgttcgaaca catccgcaaa gcaggacagg
      241 tgcgggtgcg ggtcgatggg acggtttatg acctggagca gacgccgccc ctggacgggc
      301 gcgcggctca cacgatcgaa gccgtcgtcg atcgaatcgt catccgcgag ggctcccgcg
      361 gccggctggc ggaatcgctg gcgctggcgg tgaagcaggg cgagggcgtg gtggtggcag
      421 cagtgcaggg cgcgagggaa caggggacag gggacagggt acaggataca gagtgggacg
      481 accggctctt cagcacgctc tatgcgtgcc cgaattgtcg gacgagcatt gccgagatcg
      541 agccgcggac gttcagcttc aacagcccct acggcgcgtg cccagcgtgc gacggcctgg
      601 ggtacaagct gcagttcgat cccgacttgg tcgtgccgga cataagccag tcgattgcgg
      661 gcggggcggt cgccgcctgg cggaccaaag ccgcgaccag tggtcgcggg agccgcaaga
      721 tggggacaga ggacagagga caggagacag gggacaggag acaggggaca ggggtcagga
      781 gtcagcggga ggcagtcgcg gcgttcttgg agtcgcagca gacatcgctc gatacgccgc
      841 tcaccgagat gcgtcggatg gtccgcgaga agctctttgc gggcgacggc aaggagtttc
      901 tcggcctcct gacgctgctg gaacaggagt actcgaccgc cacggatgcg gggcggaggg
      961 aggagctgga atcgttccgc ggccatgtca cttgcgtcgc gtgcggcgga gcgagacttc
     1021 gtcccgaggc cctcggcgtt cggatcggcg gccaaaatat cgcggatatc tgccgcttgg
     1081 gtattgccga agcgcgagag ttcttctgca atctcaccgc cgcaatccaa aatccaaaat
     1141 ccaaaatcca aaatgcagcg ggggaaccgc gaccactggc cacggctttg gaggccgaaa
     1201 tcgccgcccc aatcctccac gaggtttcgg cccgcctcgg cttcctggaa aaggtcggcc
     1261 tcgaatacct cacgctcgac cggcccagcg atacgctctc cggcggtgag ctgcagcggg
     1321 tccgcctggc gagcggcatc ggcagcggac tggcaggcat cctctatatc ctcgatgaac
     1381 cctccatcgg gctgcacccg cgcgacaacc agcggctgat cgatgccctg cgggacttgc
     1441 aggccggcga cagcaccgtg ctcgtcgtcg agcacgatga ggccatcatg cgccaggccg
     1501 actggttgat cgacatcggc cccggcgccg gcagccgcgg gggaagcgtc gtggccgagg
     1561 ggacgccagc cgaagtgatg gccggtccgg atacgatcac cggccgctat ttggctggcg
     1621 cgctgtcgat tcccattcct gctcagcgcc gcaaagccgc caagtcgcgg agcatcgtga
     1681 tcgaaggggt gacgacgaac aacctgcaaa acgtcacggc gacgttcccc cttgcctgcc
     1741 tgatcggcat cagcggcgtc agcggctcgg gcaaaagctc gctcatcaac gagacgctcg
     1801 cgccagcgct cgttcggcga ctcggaggaa tggcgcccaa gccggggccg cacacagccc
     1861 tccgcggcgt gagccagatc gacaaggtga tcgccatcga ccagtcgccg ctggggcgct
     1921 ccccgcggag caacgcagca acatacaccg gcgccttgga cgagattcgc aaggttttcg
     1981 ccggcacgaa ggaggcccgg cagcttggat tcaaggcgaa tcggttcagc ttt
//
LOCUS       DASUGB010001206         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1941041, whole genome shotgun sequence.
ACCESSION   DASUGB010001206 DASUGB010000000
VERSION     DASUGB010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941041"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFV87_22825"
     CDS             <1..601
                     /locus_tag="VFV87_22825"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HEX5106680.1"
                     /translation="SKTHPVWLDTKRKAVVIDGQVCLREGQLEMFACPKGTKEHESVV
                     MLDCVPQDVHAGLLALGAKPGQPVKFDPKYEPAAGDIVDIYVLWKDEQGEKHQARAQE
                     WIQYAKTEKAMPFDWVFAGSGFWTDEQTGERHYQANGGDFICVSNFPTAMLDLPVESS
                     QANADLLFHAFTEKIPPKGTKVRVVLLPRVAKKAEEPKE"
     gene            complement(681..1184)
                     /locus_tag="VFV87_22830"
     CDS             complement(681..1184)
                     /locus_tag="VFV87_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106681.1"
                     /translation="MSNPLLKPNDPRFQKPSITDAEGQNRFAEGEKSPEGGEVPAGDA
                     FTAGAAADARPYQPTFEATQRPRSRMLLTIAALGFAGVIVGLIALVGIFPAGYAFTLI
                     CVVPAATAWLLAYQDLKTMQLGAMDDSGREQTRLAYWLGLFGLVFCTGIVATAIWRQM
                     NLLPDLF"
     gene            complement(1256..2098)
                     /gene="hemQ"
                     /locus_tag="VFV87_22835"
     CDS             complement(1256..2098)
                     /gene="hemQ"
                     /locus_tag="VFV87_22835"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX5106682.1"
                     /translation="MTARPPAPQPVPISLEPTEGWHCSHLYYRFDRGALAQVSADERQ
                     AGRAELTAILDPAGDGAPQRLQTSVVSGNKADFGLMVMDPNPLKIEAVNQRLLASKLG
                     PAIVPTYSFVSITEISEYVPSVEAYSKKLIEQEGEVEGSPSHAAKVKAYGDRLPMMNR
                     QRLTPDFPPWPATCFYPMNKKRKVGENWFLTPFPERNKMMAEHAKSGMTFAGRVQQLI
                     TVGLGLEDWEWGVTLWAKNPQYLTEIVYRMRFDEASARYAEFGPFYTSYVVTPTEMLE
                     HCRV"
     gene            complement(2119..2337)
                     /locus_tag="VFV87_22840"
     CDS             complement(2119..2337)
                     /locus_tag="VFV87_22840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106683.1"
                     /translation="MIRHADDRSINELLADTDRVMQAVRQAGRDARLRHKQLGVPLVV
                     WRDGQVVEIPPEEIVVDPPTEKPLTPDS"
     gene            complement(2321..>2636)
                     /locus_tag="VFV87_22845"
     CDS             complement(2321..>2636)
                     /locus_tag="VFV87_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106684.1"
                     /translation="IAGGYEFQLFFLWVPTPEFAIQRVANRVRLGGHFVHGETIRRRF
                     YRGLQNLFNLYMPLTTDWFLYDNTQSPGESLVARGHGKLVDEVENEALWEDLRTKYDQ
                     AR"
BASE COUNT          490 a          808 c          860 g          478 t
ORIGIN      
        1 ctcgaagacc catcccgtgt ggctcgacac caagcggaag gcggtggtga tcgacgggca
       61 ggtctgcttg cgggaggggc agctcgaaat gttcgcctgc cccaagggaa ccaaggagca
      121 cgaatcggta gtgatgctcg attgcgtgcc gcaggacgtc cacgccggcc tcctggccct
      181 cggcgccaaa cctggccagc cggtgaaatt cgatccgaag tacgagccgg cggcgggcga
      241 catcgtcgat atctacgtgc tgtggaagga tgagcaaggc gagaagcacc aggctcgggc
      301 tcaggagtgg attcagtacg ccaagaccga aaaggccatg ccgttcgatt gggtcttcgc
      361 gggcagtggg ttctggaccg acgagcagac cggcgagcgg cactatcagg ccaacggcgg
      421 cgacttcatc tgcgtttcga acttcccgac ggcgatgctg gatctgccag ttgaaagctc
      481 gcaggccaac gctgacctgc tgttccacgc ctttaccgag aagattcctc ctaagggaac
      541 gaaagtccgc gtcgtcctgc tgccgcgggt ggcgaagaag gcggaggagc cgaaggaata
      601 ggagtcagga gtcaggagtc aggagtcagg agtcaggagt caggaacacc catcacttat
      661 ttacccacca cccacccgcc ctagaacaga tccggcagca gattcatctg ccgccagatg
      721 gcggtggcga caattccggt acagaaaacg aggccgaaga gcccgagcca gtaggccagt
      781 cgcgtctgct cacggccgga atcgtccatc gccccgagct gcatggtttt caggtcttga
      841 taggccaaga gccaggcggt ggcggctggg acgacgcaga tgagcgtgaa cgcatagccg
      901 gcggggaaga tgccaaccag ggcgatgagg cccacaatca cgccggcgaa gccgagcgcg
      961 gcgatcgtca ggagcatccg gctgcggggg cgctgcgtcg cctcgaacgt tggctggtaa
     1021 ggccgcgcgt ccgcggcggc tccggcagtg aaggcgtcgc ccgcgggaac ctcgcctcct
     1081 tccggagact tttcgccctc ggcgaatcgg ttctggccct ctgcatcggt gatgctaggt
     1141 ttctggaacc gcgggtcgtt cggcttgagg aggggattgg acatgtggtc gtggggtagg
     1201 cttctagcct gcccgtgtac ccgacttcag ggcaggcgag acgcccaccc cacgttcaca
     1261 cccggcaatg ctccagcatc tccgtcggcg tcacgacgta gctggtataa aacggcccga
     1321 actcggcata gcgggcgctg gcttcgtcga accgcatccg atagacgatc tcggtgaggt
     1381 actgcgggtt cttggcccac agcgtgacgc cccactccca atcttccaag ccgaggccga
     1441 cggtgatgag ctgctgcacc cggccggcga aggtcatgcc gctcttggcg tgctcggcca
     1501 tcatcttgtt ccgctcggga aaaggtgtca ggaaccaatt ctcgccgacc ttccgcttct
     1561 tgttcatcgg atagaagcag gtcgccggcc acggcggaaa gtcgggcgtc agtcgctggc
     1621 ggttcatcat cggcagccgg tcgccgtacg ccttgacctt cgcggcgtgc gaggggctgc
     1681 cctcgacctc gccttcctgc tcgatcagct tcttgctgta ggcctcgacc gacggcacgt
     1741 attcggagat ttcggtgatc gacacgaacg agtacgtcgg cacgatcgcc gggccgagct
     1801 tgctcgccag cagccgctgg ttgaccgctt cgatcttgag cggattgggg tccatcacca
     1861 tcaggccgaa atcggccttg ttgccgctga cgacgctcgt ctgcagccgc tgcggcgcgc
     1921 cgtcgccggc cggatcgaga atcgcggtca gctccgcccg gccggcctgc cgctcgtccg
     1981 cgcttacctg tgcgagcgcg ccgcggtcaa accggtaata caagtgcgag cagtgccagc
     2041 cttcggtcgg ctccaggctg atcggcacgg gctgcggggc gggagggcgg gcggtcatcg
     2101 gggcacaggg gtcaggggtc aggaatcagg ggtcagcggc ttttcggtgg gaggatcgac
     2161 cacgatttcc tcgggcggga tttcgaccac ctggccgtcg cgccacacca cgaggggcac
     2221 acccagttgc ttgtgccgca agcgggcatc gcggcctgcc tggcgaacgg cctgcatgac
     2281 gcgatcggta tctgcgagca gttcgttgat ggaccggtcg tcagcgtgcc tgatcatatt
     2341 tcgtccttag atcttcccac agcgcttcgt tctcaacttc atcgacaagt ttaccatgcc
     2401 cgcgagcaac gagggattcg ccgggactct gcgtattgtc gtacaggaac cagtcggtgg
     2461 ttagcggcat gtacaggttg aataaattct ggagcccccg atagaagcgt ctgcgaatcg
     2521 tttccccatg gacgaagtgt ccgcccagcc tcacgcggtt cgcgacgcgc tgaatcgcaa
     2581 actcgggtgt tggcacccaa agaaagaaca actgaaattc gtagccgcct gcgatc
//
LOCUS       DASUGB010001207         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_276051, whole genome shotgun sequence.
ACCESSION   DASUGB010001207 DASUGB010000000
VERSION     DASUGB010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276051"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /locus_tag="VFV87_22850"
     CDS             <1..2129
                     /locus_tag="VFV87_22850"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5106685.1"
                     /translation="HAKLRLGWTEIAGGLLVAWHSLVALVWLGEANDRQTLNALWLVI
                     GYGLTMLMLRQFIDTAAKARTAILVMIWLATTLAAFAYVQYFAIMPVVRAQYERDPEK
                     FLIEQGISSNPNSPQRQLFVNRLQSVEPLATFALTNSLAGFLAPWLMVTLAIGLAAAG
                     DKSGRRELVAAICIAAALAGCLLLTKSRTAVLASLAGVVLLAIYGTRRGALGRWIGWK
                     LPLLAAAALVVIGLAVVYVGGLDVEVLSEAPKSVLYRLEYWRSTAAMIADHPLWGCGP
                     GNFQEAYARYKLPQASEMVRDPHNFLLEVWATTGTPGIVLLLLTALAFVADLTRLQSA
                     DIAAATAEPAIAGEKDTAPRTLFFSGCIGGLVVGAALAWLSGYPLDVAADTLVPVLWL
                     LGLPLAVLAWWLLGHWVRQGELPVGAVIVAIVVLMINLLAAGAVIFPGVMLTLLVLIP
                     AAIALRQHSPNSSDSGARQDRSVNRLPIIFPLSRFSAVGLAAGAFALAAGCLKTEYEP
                     VLLARPSQLLAEQRLQQGQASEALEAAQTAAGDDPWTPEPWRIIATARLGRFLADPSE
                     SCWAEFLAAANEFQRRNPRHHLQWFERGKWHQLAWQRTGSPPRLEETLAAYRQAISRF
                     PNHALYHAQLAWALNVAGQRAAARKEADRAQALDDSMPHAEQKLRVQQIVDGHETAAG
                     FNTLRSETAEQTVAQLRTPSGRQSVP"
     gene            2282..>2538
                     /locus_tag="VFV87_22855"
     CDS             2282..>2538
                     /locus_tag="VFV87_22855"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX5106686.1"
                     /translation="MLPVTVTPVGTNGAAGHEPIGPRLSVLSSERFDFGVMDRNETMT
                     HVFEVKNVGDEVLTLKQESTSCKCTYAGAAKEELQPDETTE"
BASE COUNT          440 a          795 c          823 g          480 t
ORIGIN      
        1 cgcacgcgaa gctgcgacta ggctggacgg agatcgccgg tggactgctc gtcgcctggc
       61 attcgctcgt cgcgctagtc tggctcggcg aggccaacga ccggcagacg ctgaatgcgc
      121 tgtggctcgt catcggctat ggactgacga tgctgatgct gcggcagttc atcgataccg
      181 cggcgaaagc ccgcactgca attctcgtga tgatctggct cgcaacgacg ctggccgcat
      241 tcgcctacgt gcagtacttc gcgatcatgc cggttgtgcg ggcgcagtac gagcgagacc
      301 ccgagaagtt cttgatcgag cagggaatct cgtccaatcc caattcgcca cagcggcagt
      361 tgttcgtcaa ccggctgcaa agcgtcgagc cgctggcgac tttcgccctg acaaattcgc
      421 tggcaggatt tcttgccccg tggctgatgg tgacgctggc gatcgggctg gcagcagcgg
      481 gcgacaaatc cgggcggcgc gagttggttg ccgcgatttg cattgccgcg gccctggccg
      541 gctgcctgct gctcaccaag agccgcacgg cagttctggc gtccctggcg ggcgtcgtgc
      601 tgctggcgat ctatggcacg cggcgcggcg cgctcggccg ctggatcggc tggaagctgc
      661 cgctgctcgc ggccgcagcg ctggtcgtga ttggcctggc agtcgtctat gtcggcggcc
      721 tggatgtgga agtcctgagc gaagcgccca agtcggttct ctatcgcctc gagtattggc
      781 gttcgaccgc cgcgatgatc gccgatcatc cgctgtgggg ctgcgggcca gggaattttc
      841 aggaggcgta tgccaggtac aagctgccgc aagccagcga aatggtccgc gacccgcaca
      901 acttcctgct ggaagtctgg gcgaccaccg gaacgcccgg aattgtgctg ttgctcttga
      961 cggcgcttgc ctttgtggcc gatctgacgc gcctgcaatc ggcggacatt gcggcagcga
     1021 ccgccgaacc agcaatcgcc ggggagaaag atacggcgcc gcgtacgctc ttcttcagcg
     1081 gctgcatcgg cggactggtt gtgggcgcgg cccttgcctg gctcagtgga tatccgctcg
     1141 acgtcgcagc cgatacgctt gtgccggtgc tctggctgct cggcctgccg ctggcagtgc
     1201 tcgcctggtg gctcttgggc cactgggtcc ggcagggaga actgcctgtg ggagcagtga
     1261 tcgtggcgat tgtagttctg atgatcaacc tgctggccgc aggggcggtg atttttcccg
     1321 gcgtcatgct cacgctgctg gtactgattc ctgcggcgat cgcactgcgg cagcactcgc
     1381 caaactcttc cgacagcggc gcccgtcagg accgctctgt aaatcgcttg cccatcatat
     1441 ttccgctctc ccgtttttca gccgtgggcc tggcggccgg cgctttcgcg ctggccgcgg
     1501 gctgcctgaa gaccgagtac gagcccgtgc tgctcgcgcg gccgagtcaa ctcctcgccg
     1561 aacaacgcct gcagcaggga caagcgagtg aggccctgga agccgcccaa actgccgccg
     1621 gcgatgatcc ctggacgccg gaaccctggc ggatcatcgc gacggcgcgc ttggggcggt
     1681 ttctcgccga tccgagcgaa tcctgctggg ccgaatttct tgcagcggcg aatgagtttc
     1741 aacgccgcaa tccgcgccac cacctgcaat ggtttgaacg cggcaagtgg caccagttgg
     1801 cctggcagcg cacaggatca cccccacgtt tggaggagac tctcgcggca tatcggcaag
     1861 cgatcagccg cttccccaat cacgcgctgt atcatgcaca gttggcctgg gcgctaaatg
     1921 tggccggcca gcgtgcggcc gcccgtaagg aagccgaccg ggctcaagcc ctcgacgatt
     1981 cgatgcctca cgccgagcag aagctccgcg tccagcaaat cgtcgatggg cacgaaactg
     2041 ccgccggctt caacacgctc cgctctgaaa cggctgaaca gaccgtggcc caactgcgta
     2101 ctcccagcgg gagacaatcg gttccatgac gacaactacc acttcggcaa acgaggccgc
     2161 cgggcatacg aaatccaaag gttttccatg gatggttgtc gcggtcgccg caggaatcgt
     2221 ctccctggca gttgggggat tgattggcgc cagattagga cgcctggagt tttcccacga
     2281 ggtgttgccc gtcacggtga cgccggtggg cacaaacggg gctgccgggc acgagcccat
     2341 tggcccacga ctcagcgtcc tttccagtga gcgctttgac ttcggcgtga tggatcgcaa
     2401 cgagacgatg acgcacgtct tcgaggtgaa gaatgtcggc gatgaagttc tgacactcaa
     2461 acaggaaagc acgtcgtgca agtgcacgta tgccggcgcc gcgaaggaag agctgcagcc
     2521 cgacgaaacg accgaaat
//
LOCUS       DASUGB010001208         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_276095, whole genome shotgun sequence.
ACCESSION   DASUGB010001208 DASUGB010000000
VERSION     DASUGB010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276095"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFV87_22860"
     CDS             complement(<1..549)
                     /locus_tag="VFV87_22860"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX5106687.1"
                     /translation="MLSRLAIALVLLLAGASAAGAKEWAQKMFKAASHDFGHVAHGAK
                     AEVAFEIQNLYEEDVHIADVRTSCGCTVPTITKPTLKTWEKGEIIATLNTRSYVGQRN
                     SVLTVIIDKPYYAEVPLTIAGYIHSDVDFQPGVVSLGDVSLGSKAEQEVTITYRGRGA
                     WQVTDVRSANPHFEVELSDAMRQ"
     gene            867..1313
                     /locus_tag="VFV87_22865"
     CDS             867..1313
                     /locus_tag="VFV87_22865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX5106688.1"
                     /translation="MNRASCWHVISLIAICAATSARAEDAPDDLKLLAGTWKPAAADL
                     GGNKIDAMVLEKASFVVEGDKYTVTVNDFTEKGSFKIEPTKEPKQLDFFPTEGSNNGK
                     TFLCVYKFDGECLTICYSLDTISRPENFEPLSNTLLLVQYERVKTP"
     gene            1367..2668
                     /locus_tag="VFV87_22870"
     CDS             1367..2668
                     /locus_tag="VFV87_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5106689.1"
                     /translation="MTTKQTRRRFLQTAAAASAVFAVPQIIPARAFGANERVNLGFVG
                     VKNRGMQNMGGFAVLGSDAGKLGCAAAGLCDVDTAVLASAANRAAQSGAKPHVAGDYR
                     HLLDRKDIDAIVVSTPDHWHALITVDACRAGKDVYCEKPLTLFVTEGRRMVEVARETG
                     RVVQTGSQQRSDDRFRQACELVRNGALGKLQKVLVGIPKPNYPPLAKAVPDSDPPPEL
                     NYELWLGPAPWRPYNVNRVHYNFRFFWDYSGGQMTNFGAHHLDIAQWGLGADDSGPIA
                     IEGTGTFPKEKHLCEVTDTCRLTYSYPDGVEVILGQAQKDIPDAVTFIGEKGQIHVTR
                     GAIKADPPEILKTELGADAVKLTVSKNHYANFLDCVKSREKPICDVEIGHRSATVCHL
                     GNMAIRLGRKIQWDPAKEQILGDDGAAAMLTRPYREPWKLG"
     gene            complement(2716..4005)
                     /locus_tag="VFV87_22875"
     CDS             complement(2716..4005)
                     /locus_tag="VFV87_22875"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106690.1"
                     /translation="MLTIRCAKKQVRRGVSRRDVLKIGALGLGGLALPDILRQQAAAA
                     GSPSGKGKSVILIWLRGGASHIDSWDMKPDAPPEIRGEFHPISTNVPGIQICEHLPRQ
                     AQMMDKLAIVRGIRSNDLGDHTPHYILTGHPSRGIRPSIGSIVSYLRARDSTGALRSE
                     IPAAVTSFDYNYGFSTYTGPAHEPFYPRGEGIDNLERRRELTPGRLDNRRELLGQLDN
                     LRREIDRTRTFEAIDSFTAQALEMVTSSKARDAFDLKREPAEAHERYGKYCETFLMAR
                     RLVEAGVSVVTVKVGDWDTHERNFIDHADQLPQLDQGFHALVSDLYDRGLQDNVAVVL
                     WGEFGRAPRISRGDGRDHWPEAGAAVLAGGGWKVGQAIGETDSHAAEAKSKPYTPANI
                     FANLYRHLGIDPAIAIPDFNNRPMHLLDDRELVRELL"
BASE COUNT          742 a         1292 c         1329 g          740 t
ORIGIN      
        1 ttgccgcatc gcgtcgctca gctcgacctc gaagtgcgga ttggcgctgc ggacgtcggt
       61 gacctgccac gcccctcggc cgcgataggt gatggtcacc tcttgctcgg ccttcgaacc
      121 cagggaaacg tcgcccagcg agaccacgcc cggctggaaa tcgacgtccg aatggatgta
      181 gccggcgatc gtcagcggca cttcggcata atagggcttg tcgatgatga cagtcaggac
      241 ggagtttcgc tggccgacat acgaccgcgt gttcagcgtg gcgatgatct cgcccttctc
      301 ccaggttttg agcgtgggct tggtgatcgt cggcacggta cagccgcagc tcgtgcggac
      361 atcggcgata tgcacgtcct cttcatagag gttctggatt tcgaaagcga cctcggcctt
      421 ggcgccgtgg gcgacgtgtc cgaagtcgtg gctggccgct ttgaacatct tctgcgccca
      481 ctccttcgcg ccggcggcgg aagctccggc gagcagcagg acgagcgcga tggccaggcg
      541 tgaaagcatg ttgccattcc ccgtgagccc tgcggcgggg catcgattag cgggagtcgc
      601 tagcatcttg attctatcgc gggtgtgcat ccagaggcga ccgcccggcg tgctgccggg
      661 cacgaaccgc accgccggct cctgaggcgc gcggcgcggt tcgcttttag ggatatttcg
      721 gcctgcgcgg cggcaggctg aaagacattt ccgattgtaa ggaagctggg aaaactgcgg
      781 caacactgcg gcttggattt gacacccccg tgcttaatgg cgcacgatag gggcgctggc
      841 agtcgccccg tacggagatc ctgacgatga atcgcgcatc gtgctggcac gtgatatcgc
      901 tcattgctat ctgcgccgcg acttcagccc gggccgagga tgcgcccgat gacctcaagc
      961 tgctggccgg aacgtggaag ccggcggcag cggacctggg cggcaacaag atcgacgcca
     1021 tggttctgga gaaggcgtcg ttcgtcgtcg agggggataa atacacggtc acggtcaacg
     1081 atttcaccga gaagggatcg ttcaaaatcg aaccgaccaa ggagcccaag cagctcgact
     1141 ttttccccac cgagggcagc aacaacggca agacgttctt gtgcgtctat aagttcgacg
     1201 gcgagtgcct cacaatctgc tattcgctcg ataccatctc caggcccgag aacttcgagc
     1261 ccctcagcaa cacgctgctc ttggtacagt atgagcgggt gaaaacgccc taattcaaag
     1321 ccgcgaccag tggccgcgct tctctctccc tctgccctag ccaaacatga ccaccaaaca
     1381 aacccgccgc cgcttcctcc aaacagccgc agccgcctcg gcggtcttcg ccgtaccgca
     1441 gatcattccc gcccgcgcct tcggcgcgaa cgaacgggtc aatctcggct ttgtcggtgt
     1501 caagaaccgc gggatgcaga acatgggggg ctttgcggtc ctgggaagcg acgcgggcaa
     1561 gctcggctgt gcggcggcgg ggctgtgcga cgtggatacc gccgtgctcg cctcggcggc
     1621 caatcgcgca gcgcaatcgg gcgcgaagcc gcacgtggcc ggcgactatc gccacctgct
     1681 cgaccgcaag gacatcgacg cgatcgtcgt ctcgacgccc gaccactggc acgccctgat
     1741 cacagtcgat gcctgccggg ccggcaagga cgtttattgc gagaagccgc tcacgctgtt
     1801 tgtgacggaa ggccgccgga tggtcgaggt cgcccgcgag accggacggg tggtgcagac
     1861 cggttcgcag cagcgctcgg acgaccgctt ccgccaggcg tgtgagctgg tgcgaaacgg
     1921 ggcgctgggc aagttgcaaa aagtgctggt tggcattccc aagccgaact atccgccgct
     1981 agcgaaagcc gttcccgata gcgacccccc gccggagctc aattatgagt tgtggctcgg
     2041 cccggcgccg tggcgaccgt ataacgtcaa ccgcgtccac tacaacttcc gcttcttctg
     2101 ggactacagc ggcgggcaga tgaccaattt cggcgcgcac catctcgata ttgcccagtg
     2161 gggcctgggc gccgacgaca gcggccccat cgcgatcgag gggaccggca cgtttcccaa
     2221 ggaaaagcac ctctgtgaag tgaccgatac ctgccgcctg acttattcct acccagacgg
     2281 cgttgaggtc attctgggcc aggcgcagaa ggatattccc gatgccgtca cgttcatcgg
     2341 cgagaaaggg cagattcacg tcacccgcgg tgcaatcaaa gccgacccgc cggagattct
     2401 gaaaacagag cttggcgccg atgcggtgaa gctgacggtg agcaagaatc actacgcgaa
     2461 cttcctcgac tgcgtgaaat ctcgtgagaa gccgatctgc gacgtggaga tcggccaccg
     2521 ctcggcgacc gtttgtcacc ttggcaacat ggccatccgc ctgggacgca agatccagtg
     2581 ggaccccgcc aaagagcaga ttctgggcga cgacggggcc gccgcgatgc tcacccgccc
     2641 ctaccgcgag ccgtggaagc tggggtaacg agccaatctg ccaccgtgtg gatcgccgcc
     2701 tacggtcgat cgcagctaaa gcagctcgcg gaccaactcg cggtcgtcga gcaggtgcat
     2761 gggccggttg ttgaaatcgg ggatcgctat tgccgggtcg atgccgaggt gccggtagag
     2821 gttggcgaag atgttggcgg gtgtgtaggg cttgcttttc gcttcggccg catgggagtc
     2881 ggtctcgccg atcgcctgcc cgactttcca gccgccgcct gcgaggaccg cggcaccggc
     2941 ctcgggccag tggtcgcggc cgtcgccgcg gctgatgcgc ggagcccggc cgaactcgcc
     3001 ccacaggaca acggcgacgt tgtcctgcag gccgcggtcg tagagatcgc tgaccagcgc
     3061 gtggaagccc tggtcgagct gcgggagctg atcggcgtgg tcgatgaagt tccgctcgtg
     3121 cgtgtcccag tcgcccacct tgacggtcac gaccgaaacg cccgcctcca ccaggcggcg
     3181 ggccatgagg aatgtctcgc agtacttgcc gtagcgctcg tgggcttcag ccggctcgcg
     3241 cttgaggtcg aaggcgtcgc gagctttgga ggaggtgacc atctccaggg cctgcgccgt
     3301 gaacgagtcg atcgcctcga aggttcgcgt gcggtcgatc tcacggcgaa ggttgtcgag
     3361 ctggccgagg agctcgcggc ggttgtccaa gcggccgggc gtcagctcgc gccgccgctc
     3421 caagttgtcg atgccctcgc cccgcggata gaacggctcg tgcgccggcc cggtataggt
     3481 cgaaaagcca tagttgtagt cgaacgacgt gacggcggcc ggtatttcgc ttcgcaatgc
     3541 gcctgtcgaa tcacgagccc gcaagtagct gacgatagag ccaatgctcg gccgaatgcc
     3601 gcggctggga tgcccggtga ggatgtagtg cggcgtgtgg tcgcccaggt cgttcgagcg
     3661 gattccgcgg acgatcgcca gcttgtccat catctgcgcc tgtcgcggca gatgctcaca
     3721 gatctggatg ccgggaacgt tcgtgctgat cgggtggaac tcgccgcgga tttccggcgg
     3781 ggcatcgggc ttcatatccc aactgtcgat gtgtgaagcg ccgccgcgga gccagatgag
     3841 gatgacggac ttgcccttgc cggaagggct gccggccgca gcggcctgct ggcggaggat
     3901 gtccggcaat gcaagcccgc ccaaccccag cgcgccaatc ttcagcacat cgcggcgaga
     3961 aacacctcgc cgcacttgct tcttggcaca acgaatcgtc agcatgtccg gtctcctggc
     4021 ggaaatgtag tgcccacctt ggacgattgg tgggcacagc ccaccctacg aattaggtcg
     4081 ctacttccac acccgctttt tcc
//
LOCUS       DASUGB010001209         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1108504, whole genome shotgun sequence.
ACCESSION   DASUGB010001209 DASUGB010000000
VERSION     DASUGB010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108504"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFV87_22880"
     CDS             complement(<1..413)
                     /locus_tag="VFV87_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106691.1"
                     /translation="MAIPFQCHQCGGQFAAPDQLAGKKAKCQRCGAVVEVPAATYAGA
                     GLTTLGPATGGQMPSADPLANPLAHAAANPYGAQVANPYGPQAAGAYPQQDGGAHLQP
                     AVAATGNEIPWGWIIGGGGAVIVVAGLIGVVMMVWS"
     gene            complement(704..2572)
                     /locus_tag="VFV87_22885"
     CDS             complement(704..2572)
                     /locus_tag="VFV87_22885"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5106692.1"
                     /translation="MSAESNISSLLKGVVRDVRSSWRSLVLTDIAFKALAFIVLTPLV
                     GVLFRALLAAAGSAVVSDADILYFFLGPLGWVCIVVVGPLWLGIIALEQATLTGAICG
                     HCRGKPLGPVAALRFAAAKAWPVMGVTMRMVVFAVVAIVPFLVAAAVVYLALLTEYDI
                     NYYLTEKPPVFKWAVGIGVAIVVLMVALLLRLFTSWFFALPLVLFEDIRPRHALRLSA
                     ARAHGHRRMLLGWLVGWALAITVLSAVASGLVVLVGRALAPESTDSLRLLAVVLGLML
                     LVGAVVNLAVNLLSTVAFAAILFNLYRGLGRDGVTGTPSADFPEASGGGSSFKITRAR
                     LIAAVACGVVAAVAVDCLALRSVRLVDDVKIMAHRGSSKAAPENTLAAFRRAVEDGAD
                     WIELDVQETADGEIVVFHDNDFMKLAGRDLRIWEAALADVQDIDIGSSFDPEFKDQRV
                     PTLGEVLDECQGKIRVNIELKYYGHDQQLEQRVADIVEAHEMSSDVILMSLKMDGVKK
                     MKSIRPNWKVGLLMSVSAGNLNKIEADFLAVNVRFVNRSLIRTAHRSGKEIYVWTVDD
                     APTMSTMISQGVDGILTNKPALARSVLAQRARMSVPERLLLEFSGALGLVPEVGEQ"
     gene            complement(2868..>3251)
                     /locus_tag="VFV87_22890"
     CDS             complement(2868..>3251)
                     /locus_tag="VFV87_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106693.1"
                     /translation="ILPLALPEWQAEFCHGEMTLSGHAATLQQAALGNNLYAPLWIDL
                     NLRRVRCERTWRRLTVAENLQIVPRDVAVGYRVQFGREQWLVYRSLARWGNRTLLGQN
                     YATEFVCTRFLRTGKTHDILAIEPA"
BASE COUNT          671 a         1054 c          974 g          552 t
ORIGIN      
        1 gaccagacca tcatgacaac gccaatcagg ccagccacca cgatcaccgc ccccccgccg
       61 ccgatgatcc acccccacgg aatttcgttg cccgtagccg ccacggccgg ctgcaagtgc
      121 gccccgccat cttgctgcgg atacgcaccc gcagcctgcg gcccataggg attagcgact
      181 tgcgcaccgt acggattggc ggccgcatgc gccaacggat tcgccagggg gtccgccgat
      241 ggcatctggc ccccagtcgc tggtcccagg gtcgtcaacc cggctcccgc atacgtggcc
      301 gccggcacct cgaccaccgc cccgcaccgc tggcacttcg ctttcttccc cgccagttga
      361 tcgggcgcgg caaactgccc gccgcactgg tgacactgaa atggaatcgc catcctcgtg
      421 atcctgcacg acaagtgggt cggggcggcc gcggcggaag ctgaattgcc acccgccgcg
      481 caggtcccgc tcaagcggtc ggaacctccc aaaacgctgc cgaatccccg tcaacttacc
      541 taccgccgga gcggcaggca atgttgccag gtcgaaggag tcgggaatta actgcgcgat
      601 ccttgcttcc aagctcattc gtgtcttgcc acgaattcga aaccgcacca gtcaacgact
      661 ctcgacccac catacggtat cggcgcagtt ccataagcgt aagtcactgt tcaccgacct
      721 ccggtaccaa cccaagtgcg ccggagaact ccagcagcaa ccgctccggc acgctcatcc
      781 gggcgcgttg ggctaagaca gatcgagcca aagccggctt gttggtgagg atgccgtcaa
      841 cgccctggct aatcatcgtt gacatagtcg gcgcgtcatc gacggtccag acgtaaatct
      901 cctttccgct gcgatgggcg gtccgaatca ggctccgatt gacgaagcgg acattgacgg
      961 cgagaaagtc cgcctcgatc ttgttgagat tgccagccga aacggacatc agcagaccga
     1021 ctttccaatt cggccgaatc gacttcatct ttttgacgcc atccatcttt agcgacatca
     1081 agatgacgtc ggacgacatt tcgtgggctt ccacgatgtc ggcgacccgc tgttcgagtt
     1141 gttggtcatg tccgtagtat ttcagctcga tgttgacgcg gatctttccc tggcactcgt
     1201 caagcacttc gcccagggtg ggaacgcgct ggtctttgaa ttcgggatcg aacgaactgc
     1261 caatgtcgat atcctgcacg tcggccagtg cagcttccca gattctcaga tcccggccgg
     1321 ccagcttcat gaagtcgttg tcgtggaaga caacgatttc tccgtcggcc gtttcctgga
     1381 catcgagttc gatccaatcg gcgccgtctt cgacggcccg acggaaggcg gccagggtgt
     1441 tttccggcgc ggccttggaa gagccgcgat gcgccatgat cttcacgtcg tccaccagcc
     1501 gaacgctccg cagcgccagg cagtccacgg cgacggcagc tacaacgcca caggcaactg
     1561 cagcgatcag gcgggcgcgc gtgatcttga agctcgaacc gccgccagag gcttcgggaa
     1621 aatcagcaga cggagttccc gtgacgccgt ctctccccag tccgcggtag agattgaaga
     1681 ggatcgccgc aaatgcgacc gtactcagca aattcaccgc cagattgacg accgccccaa
     1741 cgagcaacat gaggccaaga accaccgcaa gcagccgcag cgaatcggta gactcgggag
     1801 cgagcgcgcg tccgaccagg accaccaggc cgcttgccac tgccgatagc acggtgattg
     1861 ccaaggccca gccgacgagc cagcccaaga gcatgcgccg gtgcccatgg gctctggcag
     1921 cgctcagcct gagtgcgtgg cgtgggcgga tatcttcgaa caggacgagg ggcagcgcga
     1981 agaaccaact cgtgaacagg cggagcagca acgcaaccat cagcacgacg atggcgacgc
     2041 caatgccaac cgcccattta aacaccggcg gcttctccgt gagatagtag ttgatgtcat
     2101 attcggtcaa tagcgccaga tagacaacgg ctgcggcaac caggaaggga acaatcgcca
     2161 ccacggcgaa aaccaccatg cgcatggtga cgcccatcac cggccaagct ttggccgcgg
     2221 cgaatctcag agccgccacc ggccccaatg gcttgcctct gcaatgtccg caaatggctc
     2281 ctgttagcgt cgcctgttcc agggcgatga tgcccagcca aagtggcccg acaacaacga
     2341 tgcaaaccca gcccaacggt ccgaggaaga agtacagaat gtctgcatcg gacacaaccg
     2401 cgcttccggc tgcggcgagc agcgcgcgaa acaggacgcc cacgaggggc gtcaacacga
     2461 tgaaggcaag tgccttgaag gcaatgtccg tcagaacgag ggaccgccac gaggaccgga
     2521 cgtcccgcac gactcccttc agcagcgaag aaatgttcga ttccgccgac attcagttcc
     2581 ccaagtcgcc ctgggttgga ttcttggcgt ggcggtcaaa cgggaaaatg acgtcagtgc
     2641 cccatcccga atgtcggccg ccgactacgc gttcattcgt ggagttgcgt cgagcgctcg
     2701 cgccgacgtc cgtcatctta tccgcgccgc tggcgctccg tacaaaatcc ggatcccggg
     2761 caccgcatcg ttcgctctgg gggttgtctg gaaacgtagc gggcgcaggg gcagcaatcg
     2821 tgcgcagcta gggtgcgttc gaagcggaga tcgccccacg tcgcggccta agccggctcg
     2881 atggccaaga tgtcgtgggt cttgcctgtg cggagaaacc gcgtgcagac gaactcggtt
     2941 gcgtagttct ggccgaggag cgtgcggttg ccccagcggg cgagcgagcg atagacgagc
     3001 cactgctcgc ggccgaattg cacgcggtag cccacggcga cgtcgcgggg gacaatctgc
     3061 aggttttcgg cgacggtcag ccgccgccag gtgcgctcgc agcggacacg gcggaggttg
     3121 aggtcgatcc agagcggggc gtacagattg ttgccaagag cggcttgctg gagggtggca
     3181 gcatgtcccg acagggtcat ttcgccgtgg cagaattcgg cctgccattc gggcagggcc
     3241 agcgggagaa t
//
LOCUS       DASUGB010001210         4432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1246879, whole genome shotgun sequence.
ACCESSION   DASUGB010001210 DASUGB010000000
VERSION     DASUGB010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246879"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFV87_22895"
     CDS             <1..259
                     /locus_tag="VFV87_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX5106694.1"
                     /translation="EALSDGGVRVGLPREIASALAAQTVRGAAEMVLTTGEHPAELKD
                     KVASPGGTTIAGLAALESHGLRAALIAAVEAATHRSQELGK"
     gene            371..733
                     /locus_tag="VFV87_22900"
     CDS             371..733
                     /locus_tag="VFV87_22900"
                     /inference="COORDINATES: protein motif:HMM:NF021584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEX5106695.1"
                     /translation="MEAAIGRARSEVETFIAELEAGNGEDFGVKAPVTDKGEVEHFWL
                     TDVVYRDGEFEGLIGNDPGIVSNVEFGQKWTVKKSEISDWMFMRDGKMHGNYTMRPLL
                     KTMSAAEAEQYRSMLANP"
     gene            744..998
                     /locus_tag="VFV87_22905"
     CDS             744..998
                     /locus_tag="VFV87_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106696.1"
                     /translation="MKHATDDSLDRLEPLLAQVRQIAGLVERKRGVWYRKSQAFLHFH
                     EDPAGLFADLKVGRAFERLPVDTARQRQALLRRIKQVLAD"
     gene            1208..1453
                     /locus_tag="VFV87_22910"
     CDS             1208..1453
                     /locus_tag="VFV87_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106697.1"
                     /translation="MAPIFVIVDDKHVPLYRIMWISDTPHFCGNDDCTCEGDYEVRLE
                     QDESVWASRQERDNVLKALETWYNGEREHGGHDWDET"
     gene            1568..2056
                     /locus_tag="VFV87_22915"
     CDS             1568..2056
                     /locus_tag="VFV87_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106698.1"
                     /translation="MSLFADGRYQWRETYFVLFDHKHRPKAADVQEAIAALGPRLEAH
                     EIVANKAGLLESMTVLSHADAAGMDVTYVEGEDVKEQIAELKKEWRGKKASAKEKPHL
                     ERVMHADARFDVYHFEEVSYLPDEEDDGPLDPGTLLLVLNKLARLCHGEALDPQTGEL
                     LE"
     gene            2170..2412
                     /locus_tag="VFV87_22920"
     CDS             2170..2412
                     /locus_tag="VFV87_22920"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HEX5106699.1"
                     /translation="MEVDLWSTALIFNQGHSIRVAVSSSNSPRFEPNPNTGEPHPTQG
                     KTRVATNTLHLSAEHPSAIVLPIYDGPDAISRSKAE"
     gene            complement(2830..4161)
                     /locus_tag="VFV87_22925"
     CDS             complement(2830..4161)
                     /locus_tag="VFV87_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5106700.1"
                     /translation="MPDLPSLPELRALDRRKQLVRIPYELDVPLTDRVRQLLDAPEMR
                     RLARISQLGLVGLVYPGACHSRLEHSLGVFRNALVYLQQLAGDERFAALVRSEDAEMF
                     LAAALLHDLGHWPFCHAIEDIALPNVPTHELFANSFLLEGEIADILRDEWNVQPRDVT
                     ALLSEKPRDTRQRLLKSLLSGPIDIDKMDYLVRDSLHAGVPYGRNFDQSRLIGSLCLN
                     QAGDGLAISHKGRTAAEMLVFARYVMFSEVYWHHAVRSATAMLQRAFYSLHGQLDLDQ
                     LFRLTEAAFIQQLTETAASSPAGELLSGLFGPSRKLYKRLADFSHFQGRTWYERIARQ
                     PYPWLVQCSEAFAAAASKALGRRIAPHEVLFDAPPRRREVEFDVEVHFPKENCYRRLA
                     EVSPAIHTLATRQFDDYVKRARIFVHRRLASDLARLGPLDPLLSETLRQMA"
     gene            complement(4165..>4432)
                     /locus_tag="VFV87_22930"
     CDS             complement(4165..>4432)
                     /locus_tag="VFV87_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106701.1"
                     /translation="LAPDIQPNWYTIGFVAAVAIGIGAVTAQASLDLELGTGALHYSM
                     YLLTTMVLRAILVGYQNLLWQTPPETPASAAAHWLLHAAHLVTG"
BASE COUNT          884 a         1286 c         1502 g          760 t
ORIGIN      
        1 tgaagccttg agcgacggag gcgtgcgggt gggcttgccg cgggagatcg ccagcgccct
       61 ggccgctcaa actgtccgcg gggcagccga gatggtcctg accaccggtg aacatcccgc
      121 cgagctcaag gacaaggtcg ccagtcccgg tgggacgacg atcgccggcc tggcggccct
      181 tgaatcgcac ggcctgcgcg ccgccctgat cgccgccgtc gaagccgcta cccaccgctc
      241 gcaggaactg ggcaagtagg ctactgcttt cacgcgattg acgggacttt ctacgtcaat
      301 gacagcttct tgcatttcct gctcagccaa acccgaaacg ttggtcgaag gcgggtacga
      361 cgaggctgaa atggaagcgg ccattggtcg cgcccgcagc gaagtcgaga cattcatcgc
      421 cgaattagag gccggcaacg gcgaggactt tggcgtcaag gcgcccgtta ccgacaaggg
      481 tgaggtcgag catttttggc tgaccgacgt tgtgtatcgc gacggggaat tcgaaggttt
      541 gatcggcaat gaccccggga tcgtcagcaa tgtcgagttc ggccagaagt ggacagtcaa
      601 aaagtccgaa atatcggact ggatgttcat gcgggacggc aagatgcacg gcaattacac
      661 catgcggccg ctgctaaaaa ccatgtctgc ggctgaggcc gaacaatacc gttcaatgct
      721 ggcgaacccg tgattttggc tccatgaagc atgccaccga cgactcgctc gaccgcctcg
      781 agccgctgct ggctcaggtg cggcagatcg ctgggctggt cgagcggaaa cggggcgtct
      841 ggtatcgcaa gtcccaggcg tttctgcact tccacgaaga cccagcgggg ctgtttgccg
      901 atctgaaagt cggccgggcc ttcgagcgcc tgccggtcga tacagcccgc cagcggcagg
      961 cgctgctccg gcggatcaaa caggtgctgg ccgactgact cccaaacgtc cgcgcaaccg
     1021 attccttcgc ttcctgcgga gcggcggcaa attcccagcc ggcaactccg cttcaccagc
     1081 cgcggcctgg cgacgtaaaa tagggacggc aataaacagg gacggcaatg ccggcctagc
     1141 gagttttcat gattgacgcg cggtccctcg ctccggcagg ccccgcccta ctcccaggag
     1201 cacctccgtg gcccccatct ttgtaatcgt cgatgacaag cacgttccgc tgtaccggat
     1261 catgtggatc tcggatacgc cgcacttttg cgggaacgac gattgcacgt gcgaggggga
     1321 ttacgaggtc cgcctggagc aggacgaatc ggtctgggcc tcgcgccagg agcgcgacaa
     1381 cgtgctcaag gccctggaga cctggtacaa cggcgaacgc gagcacggcg ggcatgattg
     1441 ggatgagacg tagcgggagt cgtaggacag gtttccaacc tgtccatcat ggacgggttg
     1501 gaaacccgtc ctacgcaggc tacgatgccc ggcagtcgct tctcgcccgc cagtccaggg
     1561 gccctgcatg tcgctcttcg ccgacggccg ctatcagtgg cgcgaaacct acttcgtcct
     1621 cttcgaccac aagcaccgcc ccaaggcggc ggacgtccag gaagccatcg cggccctcgg
     1681 tccgcggctg gaggcccatg agatcgtcgc caacaaggcg ggcctgctcg aatcgatgac
     1741 cgtcctgtcg cacgccgacg cggcgggaat ggacgtgacc tacgtcgagg gggaagacgt
     1801 caaggagcag atcgccgagc tgaaaaagga gtggcgcggc aagaaggcgt cggccaagga
     1861 gaagccgcac ctggagcggg tgatgcacgc cgacgcccgg ttcgacgttt atcacttcga
     1921 ggaggtgagc tatctccccg acgaagaaga cgacggcccc ctcgaccccg gcacgctgct
     1981 cctggtgctc aacaaactcg cccgcctctg ccacggcgaa gccctcgacc cgcaaacggg
     2041 ggaattactc gaatagaagc aggctcagga ccggggacaa tgtctccatc ctggggaccg
     2101 cgctcgcacg agcgtccgca gaatcaaaag caacagcacg agcctttgaa gccgggagcc
     2161 ggttaggagc tggaagtcga tttgtggagc acggccctga ttttcaacca ggggcacagc
     2221 atccgagtgg ctgtctcgtc ttcgaattcg ccgcgtttcg agccaaatcc caatacgggc
     2281 gaaccacacc ccacccaggg caagacccgc gtggccacca acacgttgca cctctcggcg
     2341 gagcatccct ccgcgattgt gctgccgatc tacgatggcc cggacgcgat tagccggagc
     2401 aaggcggaat aagcaacgac ggtcgtgtcg ctgggggaat ctttaagttt cttggcgagg
     2461 gcggaaataa tcgggcgggt tggcgcatct atatagttag atgtgatcgg atgtgaagtt
     2521 agagagggtc ggctgttcgt tgtcagcggt cgggcggaaa gggcagcgag gcgaaaaatg
     2581 agaggaaaaa tggttgacaa cgcgcaacag cgcaacatcc tcagatgggg ggtgttgcgc
     2641 ggcgcaagtt gttattcgac tagggtttgc gaagagttgc gcgaggcgga attgcgcgag
     2701 aaaaagtcgg aaagttgcgc atctcgcctg gggtcggagg gggcaatgtt gcgcaccggg
     2761 gggtattgtg cattcaacca ctggctgata ggtgcgccag gctgggcagg gtgggcaggg
     2821 cgggtgcggc taagccatct gcctgagggt ctcgctcagc agggggtcga gcggaccaag
     2881 gcgggccaaa tcggaggcga ggcggcggtg gacaaagatg cgggcccgtt tgacgtagtc
     2941 gtcaaactgt cttgtggcga gggtgtggat ggccggggag acttcggcca ggcggcggta
     3001 gcagttttcc ttggggaagt ggacctcgac gtcgaattcc acctcgcggc gccgcggcgg
     3061 ggcatcgaag aggacctcgt gcggggcgat tcgccggccg agggccttgc tggccgcggc
     3121 ggcgaaggct tcgctgcatt ggaccagcca cggatagggc tgccgggcga tccgctcgta
     3181 ccaggtgcgg ccttggaagt ggctgaaatc ggcgaggcgc ttataaagtt tgcgagacgg
     3241 accgaaaaga ccactcagca gttcgcccgc gggcgaactg gcggcggtct ccgtgagctg
     3301 ttggatgaaa gcggcttcgg tcagccggaa gagctggtcg agatcgagct ggccatgcag
     3361 cgaatagaag gcccgctgga gcatggccgt ggccgagcgg acggcgtgat gccaatagac
     3421 ttcgctgaac atgacgtagc gggcgaagac gagcatttcg gcggcggtgc ggcccttgtg
     3481 gctgatcgcc aggccgtccc ccgcctggtt caggcacagg ctgccaatca gccggctctg
     3541 gtcgaagttg cggccgtagg ggacgccggc gtgcaggctg tcgcggacga ggtagtccat
     3601 cttgtcgatg tcgatcggtc cgctgagcag gcttttgagc agccgctggc gcgtatcgcg
     3661 cggcttttcg gagagcagag ccgtcacatc ccgtggctgg acgttccatt catcgcggag
     3721 gatgtcggcg atctcgcctt ccagcagaaa gctgttggcg aacagctcgt gtgtcggcac
     3781 attgggcaag gcgatgtctt cgatcgcgtg gcaaaatggc cagtggccca gatcgtgcag
     3841 cagggccgcg gccagaaaca tctcggcatc ttcagatcga acaagcgcgg cgaaccgttc
     3901 gtcgcccgca agctgctgga gatagacgag ggcattgcga aaaacgccga gcgaatgctc
     3961 cagtcgcgag tggcaggcgc cgggataaac gagcccgacg aggccgagct ggctgatccg
     4021 cgccaggcgt cgcatttccg gcgcgtcgag gagctgccgc acgcggtcgg tcagcggcac
     4081 atccagttcg tacggaatcc gcacaagctg cttgcggcga tcgagggcgc ggagctcggg
     4141 gagcgatgga agatcgggca tgcgctagcc tgtaacaagg tgcgcggcat ggagaagcca
     4201 atgtgcagca gcgcttgccg gcgtttccgg aggagtctgc catagtagat tctggtaacc
     4261 cactagaatc gctcgcagca ccatcgtggt cagcagatac atactgtagt gcagggcccc
     4321 cgtgcctagt tccaaatcca ggctcgcctg ggcggtgacc gcgccgatgc caatggccac
     4381 agcggcgacg aatccgatgg tgtaccagtt tggctgaatg tccggtgcca gg
//
LOCUS       DASUGB010001211         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1803972, whole genome shotgun sequence.
ACCESSION   DASUGB010001211 DASUGB010000000
VERSION     DASUGB010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803972"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFV87_22935"
     CDS             complement(<1..758)
                     /locus_tag="VFV87_22935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106702.1"
                     /translation="MSEQEIFEAALERDAELRARYLDEVCGTDSELRQRIENLLKFHE
                     GAGEFFEKPAAEIVATIDQPISETPGTQIGPYKLLQQIGEGGMGVVYMAEQKEPVERR
                     VALKIIKPGMDTRQVIARFEAERQALSLMDHPNIAKVLDAGTTDSGRPYFVMELVKGQ
                     PITQYCDEHHLTPRQRLELLLPVCQAIQHAHQKGIIHRDIKPTNILVAEYDQKAVPKV
                     IDFGVAKAISQSLTEKTMFTGLGQIVGTLEYMSP"
     gene            complement(858..1424)
                     /locus_tag="VFV87_22940"
     CDS             complement(858..1424)
                     /locus_tag="VFV87_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5106703.1"
                     /translation="MSDVTQILSQIESGDPSAADKLLPLVYEELRKLAAAKLSQEKPG
                     QTLQATALVHDAYIRLVDAERAQQWDSRGHFFAAAAEAMRRILVQKARRKGRQKHGGD
                     HKRQDLDDVEIVAPATSEEILAVSEALDKFESINQPAAQLVKLRYFAGMTIPEAAESL
                     GISPRKANQLWAYARAWLLAEMDSDVLA"
     gene            complement(1734..>2778)
                     /locus_tag="VFV87_22945"
     CDS             complement(1734..>2778)
                     /locus_tag="VFV87_22945"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX5106704.1"
                     /translation="DKGRLKTSAGNLPPFNLDGLPNAGGPSPDLFLAGDIRANEQVGL
                     TAMHTLFVREHNRLAARIAQVFPHANDEQVYQLARKIVGAELQIVTYNEFLPALLGPY
                     APSLSQYAGWDPEVNPSITNEFSTALFRVGHTMLSPNLRLADGKGAAGALPLRGAFFN
                     PDILADNPQNVDYLLAGFTQQQAQEIDNFLVDDVRNFLFGPPGAGGLDLASLNIQRGR
                     DHGLPTYNGLRMAYGLEPAKEFGDISSNAVVADALAAVYKSPSDVDSWVGALAEDHLP
                     GASVGPLVAAGLVDQFTRLRDGDPFFHLNDLDLTHSRIRTIVDLRDLRLCQVIRWNTA
                     INKLPSNVFFVEE"
BASE COUNT          499 a          835 c          856 g          588 t
ORIGIN      
        1 tcgggactca tgtattcgag cgtgccgacg atctggccca ggccggtgaa catcgtcttt
       61 tcggtcagcg actggctgat cgccttggcc acgccgaagt cgatcacctt gggcacagct
      121 ttctggtcgt attcggcgac gaggatgttc gtcggcttga tgtcgcggtg gatgatcccc
      181 ttttgatggg catgctggat cgcctggcag accggcagca agagttccag gcgctggcgg
      241 ggcgtgaggt ggtgctcgtc gcagtactgg gtgatcggct ggcctttgac cagttccatg
      301 acgaagtagg gccggccgct gtccgtggtc ccggcatcga gcactttggc aatgtttgga
      361 tggtccatca gcgacaacgc ttgtcgttcc gcctcaaacc gcgcgatcac ctgccgcgta
      421 tccatacccg gcttgatgat cttgagggcc acccgccgct cgaccggttc cttctgctcg
      481 gccatataca ctacgcccat ccccccttcg ccgatctgct gcagcagctt gtaggggccg
      541 atttgagtac cgggcgtttc ggagatgggt tggtcgattg tggcaacgat ttcggcagca
      601 ggcttttcaa agaattctcc cgctccttcg tggaacttca ggagattctc gatccgctgt
      661 cgcagctcag agtcggttcc gcaaacctcg tccaaatacc gggctcgcaa ttcggcatcc
      721 cgctcaaggg ctgcttcgaa gatttcctgt tcgctcatcg ctcggcctca tttcacgcct
      781 tctatagttc aatgtgccat ttctgcctta gtcgcgcaaa gattccaaag aaatctcgat
      841 ttgtctgtcg cgaccgacta tgcaagcaca tcggaatcca tctcggcaag cagccaggca
      901 cgagcgtaag cccagagctg gtttgccttt cggggcgaaa tgccaaggga ctcagccgcc
      961 tcaggaatgg tcattccagc aaagtaccgg agctttacaa gttgagccgc gggctggtta
     1021 atcgactcga atttgtccag cgcctcactg actgccagaa tctcttccga ggtagctggt
     1081 gcgacgattt ccacgtcgtc cagatcctgg cgtttgtgat caccgccatg tttctggcgt
     1141 cccttgcgcc gtgccttttg gaccagaatc cgccgcatgg cctcggctgc ggccgcgaag
     1201 aagtggccgc gggagtccca ctgctgcgct ctttccgcat ccaccagccg aatgtaggcg
     1261 tcatgcacaa gggcggttgc ttgcagcgtt tgccctggct tctcctggga aagtttcgca
     1321 gcagccagct tccgcagttc ctcgtaaacg agcggcaaga gtttgtccgc ggctgatgga
     1381 tcgcccgatt cgatctgcga caagatttgg gtcacatcgg acatggccct cattgtcatc
     1441 gggccaagac cgactcgcaa tcgaccggga ttccgagtgc aggattagcg tcgccaacga
     1501 cagaataacg ccggtcgttt gggcgcttcg gtcccatcgc ccggacttag ttcgtcgggg
     1561 tgcatcatcg aagcgccgta gcctggccat caaacggctc ggttattcgt gtgcgacggc
     1621 ctagatttgc aacggtatgc gggtcgaact ggacatggcc ccgttgcgca gggccatgtc
     1681 cgggtgtgca attcagacca cgcgacgccg ccgtttgccc ggcgagggcc gcgttactcc
     1741 tccacgaaga agacgttgct gggcagcttg ttgattgccg tgttccagcg gatcacttgg
     1801 cacagtcgga ggtcccgtag atcgacgatc gtgcggattc gcgaatgggt gaggtcgagg
     1861 tcgttcaagt ggaagaacgg gtcgccgtcc cgcaggcgcg tgaactgatc gaccagccca
     1921 gcggcgacca gcgggccaac gctcgccccc ggcaggtggt cctccgccag cgcgccgacc
     1981 caggaatcga catcgctggg cgacttgtag acagcagcca gcgcgtcggc gacgacagca
     2041 ttggaactga tgtcaccgaa ctcttttgcc ggttccagac cataggccat acgcagcccg
     2101 ttgtacgtcg ggagcccgtg atcgcggccg cgctgaatgt tgagcgacgc cagatccagt
     2161 ccccccgcgc ccggcgggcc gaacagaaag ttccgcacat catcgacgag gaagttgtcg
     2221 atttcctgcg cttgttgctg ggtgaagccg gccagcaggt agtcgacgtt ttgcgggttg
     2281 tccgccagga tgtcggggtt gaagaacgcg ccccgcaacg gcagcgcgcc agcggctccc
     2341 ttcccgtcgg ccagccgtag attcggcgaa agcatcgtgt ggccaacgcg aaacagggcg
     2401 gtcgaaaact cgttggtgat tgacggatta acctccgggt cccagccggc gtattgcgac
     2461 agtgagggcg catacggccc cagcagcgcg ggcaggaact cgttgtaggt tacgatctgc
     2521 agttcggccc ccacaatttt gcgggccaac tgatagacct gttcgtcgtt ggcatgggga
     2581 aagacctgcg cgatccgcgc cgccagacgg ttgtgctccc gcacaaagag cgtgtgcatc
     2641 gctgtcaagc cgacctgctc gttggcgcga atgtcgccgg ccaggaacaa gtcggggctg
     2701 ggcccgccgg cattggggag gccgtcgaga ttgaaggggg gcagattgcc ggcgctggtc
     2761 ttgagccggc ccttatca
//
LOCUS       DASUGB010001212         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1941429, whole genome shotgun sequence.
ACCESSION   DASUGB010001212 DASUGB010000000
VERSION     DASUGB010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941429"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /gene="nadB"
                     /locus_tag="VFV87_22950"
     CDS             complement(<1..1389)
                     /gene="nadB"
                     /locus_tag="VFV87_22950"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5106705.1"
                     /translation="MSPLTPRYLVPFQPRELPHFFADVLVIGGGLAGLRAANAVDPRL
                     SVLVVTKDAIDQSNSNYAQGGIAGVLDPEDRFDLHVSDTLVAGGTLCDPAVVEMVIRE
                     APARIQELIDWGTHFDEQAGRIVLGREGGHSKSRIVHALGDMTGKEVMRAVIAHTRRQ
                     RHVRIWENTFTLDLLTHEGVCRGAIVATQFGEKMLVWARQTILATGGCGQVYRESTNP
                     PVATGDGHAMAYRAGARLRDMEFVQFHPTVLYIAGGGRSLITEAIRGEGGRLVDRDGV
                     RFMPQYDERAELAPRDIVSQAIVSQMEKTRHPCVYLDVTHLDPQFVLSRFPGIAAACR
                     MFDINITRDKIPVRPGAHYMIGGVTVDLEGRTTIPGLWAAGEVTSTGLHGANRLASNS
                     LLEGLVYGAHTGRGASQALLEPNGEFRVYPLENPVVAPPAEPLDLPDILNSLKSLMGR
                     QCGVRRSAEGLSD"
     gene            complement(1420..>4509)
                     /locus_tag="VFV87_22955"
     CDS             complement(1420..>4509)
                     /locus_tag="VFV87_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106706.1"
                     /translation="GGLRYQCTLPPTDTVGLTAATDGLRQDATYRIVAGDAETPAYVL
                     AVVAAPTIIVDRLEYQYPAYTKKAPETVPQTGDIKALEGTRVTVHAVANQPIKSAWLE
                     FDPGVKDGVPEVVQLAAESERAWGTIVLQLKPDRKTPWHVAYQVRFYNERSQRSQQPI
                     IHKIEVIRDLPPEVQLLQPQRLKVEVPEDGEQAIEVRALDPDYGLSRVAIEGSAAGKD
                     AIAIELLEQNEGQPPQVTKRYAFKPREHQLQAGDELKYVALAADNRTSPLTGQPEPNI
                     TKTAEYTIVVVPPRQQGEQANNPNERPMPPMQGEQENQPQQGAGEKPPMPMNDGNPPD
                     MNPPKQGEQENMPPKQGEQEQKPNQQPMQGDQQQGDKQQGDKQQGDKQQGDKQQGDKS
                     QSGNAQQGDKQQGDKQQGDQSQAGGDPQQGQPMPGQQGDKSGSPQQGQPQAGDGGSGQ
                     AGDPQQGQPASGGGQAGTQQGNPSGQPSGDSQQPAGQAGDPNADATGQPNSAGGPSGK
                     AHDGQAFEEILKDIQRDGQGQPNQGQPNQGQPNQGQPKPGQGEPQQGETAGDKTGQPM
                     PGQTAGQPMQDQPMPGQPGNEKGQGASGQQPMGQKPEAGDDATGQGTTQQGDQPQKSA
                     QSGAKGGGTDPKSNLGGNDQKLNPAEKGAGAAGEKADPNQQLPGDKQGQNPSEQKSQG
                     NEGGAGQGADKTDKAEASEPGAGEKKDPGAGESGDEGAGNNSKDKTGNGQGQEANRDK
                     PKELQSDSSKPQEGEASPPSGSKRQSDSQGDTSGDQSGGGGKGAGQSAGQEGNDSAGS
                     KSAADQGAGAANETGAGETGSQAGAQQQAAGKTGQTGDKTGEGSKTQASEQGSKSGQP
                     GDPGGKSGDLNKGDGNKPGKSGGAGPVIGGGGDGDRPKLDPMQHPEAEAADKANLEYS
                     RKATEMVLQRLKDQQQQADPELLDKLGWTEDDLAEFVRRWDALQKSAQQTPEGQRELD
                     EALRSLGLRDPANRQRAGGSTSDNQRDLRDAGGRSNPPAKYRDLFDAFRKGAARASQ"
BASE COUNT          514 a         1559 c         1467 g          969 t
ORIGIN      
        1 gtcgctcagg ccttcggcac tgcgccgcac gccgcactgc cggcccatca ggctcttgag
       61 cgaattgaga atgtcgggga ggtccagcgg ctcagcgggg ggcgcaacaa cgggattctc
      121 cagcggataa acgcgaaact cgccgtttgg ctccaataac gcttgcgacg ctccgcggcc
      181 ggtatgggcg ccatagacca gcccctccag caggctgttc gacgccaggc gattcgcgcc
      241 gtgcaggccg gtggaggtga cctcacccgc ggcccagagg ccgggaatcg tcgtccgccc
      301 ctccagatcg acggtcacgc cgccgatcat gtagtgcgcg ccgggtcgga cgggaatctt
      361 gtctctggtg atgttgatat cgaacatccg gcaggccgcg gcgatgccgg ggaagcggct
      421 gaggacgaac tgcgggtcca aatgcgtgac gtcgagatag acgcacggat gtcgcgtctt
      481 ctccatctgg ctgacgatcg cctgcgagac gatgtcgcgc ggcgcgagct cggcccgctc
      541 gtcgtactgc ggcatgaacc ttacgccgtc gcgatcgacc agccggcccc cttcgccgcg
      601 gatcgcctcg gtgatcaggc tgcggccgcc cccagcgatg tacaggaccg tcggatgaaa
      661 ctgcacgaac tccatatcgc gcagccgggc gcccgcgcga taggccatcg cgtggccgtc
      721 gccggtggcg accggtgggt tcgtcgattc gcgatacacc tgcccgcagc cgccggtggc
      781 cagaatcgtc tgccgggccc agacgagcat cttctcgccg aactgcgtgg cgacgatcgc
      841 cccgcggcag acgccttcgt gcgtcagcag gtcgagcgtg aacgtgtttt cccagatccg
      901 cacgtgccgc tgccggcggg tgtgggcgat gacggcccgc atcacctcct tgccggtcat
      961 gtcgcccagg gcgtggacga tgcggctttt gctgtgcccc ccttcgcggc caaggacgat
     1021 gcggccagcc tgctcgtcga agtgcgtgcc ccagtcgatc agctcctgga tccgcgccgg
     1081 ggcctcgcga atgaccatct caacgacggc cggatcgcag agcgtgccgc cggcaacgag
     1141 cgtgtcgctg acgtgcagat caaagcggtc ctccgggtcg agcacgccgg cgatgccccc
     1201 ctgcgcgtag ttgctgttcg actggtcgat ggcgtccttc gtcacgacca gcacgctcag
     1261 ccgcggatcg accgcgttgg ccgcccgcag tcccgccagg ccgccgccga tgaccagcac
     1321 gtccgcaaag aagtgcggca gctcgcgagg ctggaagggg acgagatagc ggggcgtgag
     1381 gggagacatc ggccccattt tagcacgccc tcgaaggatc tactggctcg cacgcgccgc
     1441 gcctttgcgg aaggcgtcga acaggtcgcg gtacttggcc ggcggattgc tgcggccgcc
     1501 ggcatcgcgg aggtcgcgct ggttgtcgct ggtgctgccg ccggcgcgct ggcgattggc
     1561 cgggtcgcgg aggccgaggc tgcgcagggc ttcgtccagc tcgcgctggc cttcgggcgt
     1621 ctgctgggcc gatttctgca gcgcatccca gcggcggaca aactccgcca ggtcgtcctc
     1681 cgtccagcca agcttgtcga gcagttccgg atcggcctgt tgctgctggt ccttcaaacg
     1741 ctgcaggacc atctcggtcg ccttgcggga atactccagg ttcgccttgt cggcggcttc
     1801 ggcttcgggg tgctgcatgg gatcgagctt cggccggtcg ccatcgccgc cgccgccgat
     1861 caccggacca gcgccgccgc tcttgccggg cttgttgccg tcgcctttgt ttagatcgcc
     1921 tgacttgccg ccgggatcgc ccggttggcc ggacttgctg ccttgctcgc tggcctgggt
     1981 cttcgaccct tcgcccgttt tgtcgccggt ttggcccgtc ttgccggcgg cctgctgttg
     2041 agcgcctgcc tggctgccgg tttcgccagc gccggtttcg ttcgcggctc cagcgccttg
     2101 gtcggcggcg ctcttgctgc cggcgctgtc gttgccttcc tgaccggccg attgccccgc
     2161 gcccttgccg ccgccgccgc tctggtcgcc ggaggtatcg ccctggctgt ccgattgccg
     2221 cttgctgccg ctgggggggc tcgcttcgcc ttcctgcggc ttggagctat ccgattgaag
     2281 ctccttgggc ttgtcgcgat tggcttcctg cccctgaccg ttgccggtct tgtccttgga
     2341 gttgttgccc gctccttcgt cgcccgattc gcccgcaccg gggtccttct tctcgcccgc
     2401 gccgggctcg gaagcttcgg ccttgtcggt cttgtccgcg ccttggccgg cgccgccttc
     2461 gttgccttgg ctcttctgct cggacggatt ctggccttgc ttatcgccgg gaagttgctg
     2521 gttgggatcg gccttctcgc cggcagcccc agcgcctttc tcggccggat tcagcttctg
     2581 gtcgttgccg ccgagattcg acttgggatc ggtcccgccc cctttcgcgc cggactgagc
     2641 cgacttctgt ggctggtcgc cttgctgcgt cgtgccctgt ccggtggcat catcgcccgc
     2701 ttcgggcttc tggcccatcg gttgctggcc ggaagcgcct tgtcccttct cgttgccggg
     2761 ctggccgggc atgggttgat cctgcatagg ctgaccagca gtttggcccg gcatcggctg
     2821 gccggtcttg tcgccggcag tctcgccctg ctgaggttcg ccctgaccgg gtttcggctg
     2881 gccttggttc ggctggcctt gattcggctg gccttgattc ggctggcctt ggccgtcgcg
     2941 ctggatgtcc ttcaagattt cttcgaacgc ctggccgtcg tgggccttgc cgctgggccc
     3001 gccggcggaa tttggctggc cggtggcgtc ggcattggga tcgccggcct gcccagccgg
     3061 ctgctggctg tcgccggacg gctggccgct cggattgcct tgctgagtgc cggcctggcc
     3121 gccgccgctg gccggctggc cctgctgagg gtcgcccgcc tggccgctgc cgccgtcgcc
     3181 cgcctgaggc tgaccctgct gcggactgcc ggacttatcg ccctgctggc caggcatcgg
     3241 ctgaccctgc tggggatcgc cgcccgcttg cgattgatcg ccctgttgct tgtcgccctg
     3301 ttgcttatca ccttgctggg cattgcctga ttgcgacttg tcgccttgct gtttgtcgcc
     3361 ttgctgttta tctccctgtt gtttgtcacc ctgttgtttg tcaccctgtt gttgatcgcc
     3421 ctgcatcggc tgttgattcg gcttctgttc ctgctcgcct tgctttggcg gcatattctc
     3481 ctgctcgcct tgctttggcg gattcatgtc aggggggttg ccatcgttca tcggcatggg
     3541 cggcttctcg ccggcgcctt gctgcggctg gttctcctgc tcgccctgca tgggcggcat
     3601 cggccgctcg ttcggattgt tggcctgctc gccctgctgc cggggcggga cgacgacaat
     3661 cgtgtactcc gccgtcttgg tgatgttggg ctccggctgg cccgtgagcg gactggttcg
     3721 gttgtctgcc gccagcgcga cgtacttcag ctcgtccccc gcctgaagct gatgctcgcg
     3781 cggcttgaac gcataccgct tcgttacttg cggcggctgg ccttcattct gctccagcag
     3841 ctcaatcgcg atcgcgtcct tgcccgccgc gctcccctca attgccacgc gcgacaggcc
     3901 ataatccgga tcgagcgccc gcacctcgat cgcctgctcg ccatcctcag gcacttccac
     3961 cttcagccgc tgcggctgca agagctgcac ttccggcggc aggtcgcgaa tgacctcgat
     4021 tttgtggatg atcggctgct ggctccgctg gctgcgctcg ttgtagaacc gcacctgata
     4081 cgccacatgc cagggcgttt tgcgatccgg cttgagctgc aaaacgatcg tgccccaggc
     4141 ccgctcgctc tcggccgcaa gctgcacaac ctctggcacg ccgtccttca cacccggatc
     4201 gaattccagc caggccgatt tgatcggctg attggcgacc gcgtgcaccg tgacccgcgt
     4261 cccctccagg gccttgatat ctcccgtttg cggcaccgtt tccggggcct tctttgtgta
     4321 cgccggatac tgatattcca gccgatcgac gatgatcgtc ggcgcggcga cgacggcgag
     4381 cacgtacgca ggcgtttcgg catcgcccgc gacaatccga tacgtcgcat cctgccgcag
     4441 gccgtcggtc gcggcggtga gtccgactgt atccgtcggc ggcagcgtgc attgataccg
     4501 caggccacc
//
LOCUS       DASUGB010001213         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1804106, whole genome shotgun sequence.
ACCESSION   DASUGB010001213 DASUGB010000000
VERSION     DASUGB010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804106"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..881
                     /locus_tag="VFV87_22960"
     CDS             456..881
                     /locus_tag="VFV87_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5106707.1"
                     /translation="MELATGQRVTATVDWADAGEIGARFKQPIDVLALINRKLVSQPV
                     DRRSMPRVEIRCAAYIKRGEDFLAATVRNVSARGLQLEGDSLPPVGSYVSLFVEGLNV
                     PPGEVAWRKDDLAGIELFEELRWSSIMPWIREQMRRQPQ"
     gene            986..1126
                     /locus_tag="VFV87_22965"
     CDS             986..1126
                     /locus_tag="VFV87_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106708.1"
                     /translation="MGNRDSAFSRSYRGRRSFRLNPTVRAIAFIALIAAFVALILWFL
                     DF"
     gene            1285..2556
                     /locus_tag="VFV87_22970"
     CDS             1285..2556
                     /locus_tag="VFV87_22970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5106709.1"
                     /translation="MHANILGSLLLAAVAAASPAEALQQQTGPSSFEEFQQLRTTLPV
                     PAEYAVLLNPSELVGVDDYPRDAMEKDEQGDVKTWLLVGADGKAERCGIVASSGSTSL
                     DAQTCKVLISNARFRPAHLANGSLVSSIFFQALAWRLEDVEKEEAQKEELQALTRLRS
                     EMQRPAKEAKLLNGDTVAGAMDYPKTAIAKEQTGRTVMLLLVGADGRVTRCAIEESSG
                     SDDLDEQSCNLMIANAHFDPARNRRNYPVKSVLKQTVNWQLQSSSIRQKDWIIRASFL
                     VGPQAQIRSCKSEFLVNETWTEASQKVCERSGSWYVLNVAKEKSKAVDTLVVVENWQL
                     TTPDHSIPNVGRNPGEVLVLSRSATATYDGDGKRTSCTAVSHYGIPEIQPDLCVSGGI
                     PDPLPEGVVQPKDGPVTLRVLLAVYLKDEPG"
     gene            complement(2566..>2982)
                     /locus_tag="VFV87_22975"
     CDS             complement(2566..>2982)
                     /locus_tag="VFV87_22975"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106710.1"
                     /translation="WTSSLPSRFVAELPDAHIEEETTMSGGESLWRAQWSERADPFAH
                     LGPARSMRSSTRGPGWQRAASSGRFNPEPQRVIEVRASAVSLGNKGRGDLALGQRVFH
                     EKFGHGTIAAIEGNKLEIDFEHAGRKKVLDSFVSVG"
BASE COUNT          607 a          884 c          908 g          583 t
ORIGIN      
        1 ggcctgcgtc gtcgtgtcga cctgctgcct ttgccgcacc gtgtcgaaga cgttcatcat
       61 cgtctgagtc gattgggccg ctgcgggcgc cgcgaccgtg gccaggagaa agagcggaag
      121 tgtcgaatga cgcatggagg cgcctccttt gccgacgact ctataccgcc gcaggtgcga
      181 tgtcgttagc gacttgttag ccctcgcggg cgcacgctcg cactccacga tgccggatat
      241 ctcgaatggc tgaagcgacc tcagtcacga ccttcgtcat gggaccgcgg ctcccgtggc
      301 ccgacgacgc gcccgaagcc ggcgaattgg ggcccttcga cgctgccgtg attgtcgatg
      361 gagacgcacg caaggactgc tcggtccgga agatatccgc cctcggggca acgcttcgaa
      421 caggtgtcgg aaaatcccca ggttcggagg ttgccgtgga gcttgcgacc ggccagcggg
      481 tcaccgcaac cgtcgattgg gccgatgccg gcgaaatcgg cgcgcgattc aagcagccga
      541 tcgacgtcct cgccctcatc aaccgcaagc tcgtcagcca gccggtcgac cgccggtcga
      601 tgccgcgcgt cgagatacgc tgcgccgcct acatcaagcg cggcgaggat ttccttgcag
      661 ccactgtccg caacgtgtcg gctcgcgggc tccagctgga gggcgactcc ctccccccgg
      721 tcggaagcta cgtgtcgctc ttcgtggaag ggctcaacgt tcctccgggc gaggtggcat
      781 ggagaaagga cgatctggcg ggaatcgagc tcttcgagga gctccgctgg tcgtcgatca
      841 tgccctggat ccgcgagcag atgcgcaggc agccgcaata gctagcgcaa gccgctgaat
      901 caggcgattt tggtagtgct gttaacctgt gatagttaac ggtcggtaac tacagtgatt
      961 tcagcgcgat gcgtagcagg actcgatggg aaaccgggac agtgcgttca gccgaagtta
     1021 tcgcggacgc cggtcgttcc ggctcaatcc caccgtccgg gccatcgcct tcatcgccct
     1081 gatcgcggca ttcgtcgccc taatactctg gttcctcgac ttttagctat ttttccatgt
     1141 cgcgaccgcg gcgcatcgcg atgcggcaaa cggttcgagt tgttccaacg cacaacagac
     1201 ggcaggcatg tcgaccaaat taagggcctc gatgggtcct gaaaattgat tcaaacactg
     1261 gagtaaattg catattgttg attaatgcac gccaacattc ttggctcgct gcttcttgcc
     1321 gccgtcgccg ccgcgtcacc ggcggaagct ttgcaacagc aaacgggccc gtcttcgttt
     1381 gaggagtttc aacagctgag gaccacattg cccgtccctg ctgaatatgc cgtcctgttg
     1441 aacccgtctg aacttgtagg cgtcgatgac tatccaagag acgctatgga aaaagacgag
     1501 cagggcgacg tcaagacgtg gttgctggtt ggcgctgacg gcaaggcaga acgctgcggt
     1561 attgtcgcga gttctggctc cacctcactt gatgcgcaaa cctgcaaggt actgatttcc
     1621 aacgcgagat tccggcctgc ccatctggca aacgggagct tggtgagtag catcttcttt
     1681 caggctttag cgtggcgact cgaggatgtg gaaaaggaag aggcgcagaa ggaggaattg
     1741 caggcgctga cccgtctcag atccgaaatg cagcgcccgg caaaggaagc aaagctgctc
     1801 aatggcgaca cggtcgccgg tgcgatggac tatcccaaaa ccgcgatcgc gaaggagcag
     1861 acgggacgca ccgtcatgct gctgctcgtt ggagccgacg gtagagtgac ccgatgtgcg
     1921 attgaagaat cgtcgggatc cgacgacctc gatgagcaat cctgcaatct catgatcgcc
     1981 aatgcgcatt tcgacccggc gcgcaatcgt cgcaattatc cggtaaaaag cgttcttaag
     2041 cagacggtta actggcaatt gcaatcatcg agcattcgcc agaaagactg gatcatacga
     2101 gcaagcttcc tcgtcgggcc tcaggcacaa attcgcagct gcaaaagcga atttcttgtc
     2161 aacgagacgt ggaccgaggc ctcgcaaaag gtgtgcgaaa ggtcaggcag ttggtatgtc
     2221 ttgaacgtgg caaaagagaa gtcgaaggcc gtcgatactc ttgttgtcgt cgagaattgg
     2281 caacttacga ctcccgatca ttctatcccg aatgttggga ggaatccggg agaagtgctt
     2341 gtcctgagcc gatcggcgac cgccacatac gacggggacg gtaaacgcac gagctgcact
     2401 gccgtaagcc actacggaat tccggaaatc cagccagatc tgtgtgttag cgggggaata
     2461 cccgacccgc ttccggaagg cgtggtccaa ccgaaagatg gaccggtgac ccttcgcgtc
     2521 cttttagcgg tctatttgaa ggacgagcca ggctgatcgg ccgggtcaac ccacactgac
     2581 gaagctgtcg agcaccttct tccggccagc gtgctcgaag tcgatttcga gcttgttgcc
     2641 ttcgatcgcg gcgatcgtgc cgtggccgaa cttctcgtga aacacccgct ggcccagcgc
     2701 aaggtcgccg cggcccttgt tgccgaggct gaccgcgctg gcgcgaactt cgatcacgcg
     2761 ctgcggctcg ggattgaatc tgccggacga ggccgcgcgc tgccagccgg gcccgcgggt
     2821 cgatgaccgc atcgaccgcg ccgggccgag atgcgcgaac gggtcggccc gctcagacca
     2881 ttgcgcccgc cacaggctct cgccgccgct cattgtcgtc tcttcctcga tgtgcgcatc
     2941 gggaagctcg gcaacgaagc ggctcggaag cgaacttgtc ca
//
LOCUS       DASUGB010001214         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_831596, whole genome shotgun sequence.
ACCESSION   DASUGB010001214 DASUGB010000000
VERSION     DASUGB010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831596"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFV87_22980"
     CDS             complement(<1..219)
                     /locus_tag="VFV87_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106711.1"
                     /translation="MQPEVTPMMGAFGAVAMLVLGAGILCWSLAALRVAIGWRIVPQE
                     LLPAGLDLLRFFRVSPGLPLIEWRPRRPV"
     gene            complement(221..847)
                     /gene="pgsA"
                     /locus_tag="VFV87_22985"
     CDS             complement(221..847)
                     /gene="pgsA"
                     /locus_tag="VFV87_22985"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5106712.1"
                     /translation="MASTKTASTAIFNVPNVLTAARFVLSIVVFVLIPLEQYAAAVVV
                     FVIAAGTDWIDGWYARKYQQVTKLGRIFDPFVDKVIICGTFIFLAAEQGSGITPWMAV
                     VVVGREMLVTAIRGFIEQQGGDFSAQMAGKLKMVFQCVAAVASLIALRHYALEVSPEA
                     PREWLAEPLPAWLFWTLHVSVALAVISTVYSGAEYIVAAARILGKQGR"
     gene            complement(884..1135)
                     /locus_tag="VFV87_22990"
     CDS             complement(884..1135)
                     /locus_tag="VFV87_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5106713.1"
                     /translation="MKYTVIWKPSALDRLTEIWLAAEDKDGVSRAVTKIDQFLGQQSA
                     AGESYFEGTRLIVAPPLVVVYEVDHGDRRATVSRVAYRP"
     gene            complement(1147..1398)
                     /locus_tag="VFV87_22995"
     CDS             complement(1147..1398)
                     /locus_tag="VFV87_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106714.1"
                     /translation="MSTVLIDASTAAKLKQPQTTVEVRTEDGQLVGMFTPMREATPED
                     YEWARQQFTREEIAKARAETGGYTTAEVLEHLRKLESEA"
     gene            complement(1428..>2373)
                     /gene="rimO"
                     /locus_tag="VFV87_23000"
     CDS             complement(1428..>2373)
                     /gene="rimO"
                     /locus_tag="VFV87_23000"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5106715.1"
                     /translation="KISEGCDRLCTFCAIPKMRGKHASKPIEEVIAEAQQLAADGVRE
                     LIVVAQDTTYYGLDLYGETRLTELLRKLEEVRGIDWIRLMYFYPMYIDDELIHTIAES
                     RKILPYIDIPLQHASDTMLKRMARRVSRAETEELLDRLRSRIEGLALRTTFITGFPGE
                     TEADVDQLAEFIEKYKFERMGVFTYSFESDTPAANLPNPVPEGVMEARRERLMQLQQQ
                     IAFDYNQRQVGQTRDVILDQPVEGERGVWLGRSTADAPDVDGLVFVTADQHKLRAGDI
                     VPVEIVATQDYDLVGVAAGKPRGREQVVSAPLLALGKV"
BASE COUNT          437 a          824 c          684 g          428 t
ORIGIN      
        1 caccggccgc cgtggccgcc actcaatcag cggcagcccg ggcgagacac gaaagaatcg
       61 caacaaatcc aaaccagccg gcagcagctc ctgcggcacg atgcgccagc cgatcgccac
      121 gcgcagcgcc gccagcgacc agcagaggat gcccgccccg agcaccagca tggccaccgc
      181 gccaaaggcg cccatcatcg gcgtgacttc gggctgcacg tcagcgtcct tgcttgccga
      241 ggatgcgggc cgccgccacg atatactccg cgccggaata aaccgtcgaa atcaccgcca
      301 gcgcgaccga aacgtgcagc gtccaaaaga gccacgccgg cagcggctcg gcaagccact
      361 cgcgaggggc ctcggggctg acctccaggg cgtaatgccg cagcgcgatc aggctcgcca
      421 ccgccgcgac gcactgaaac accatcttca acttgccggc catctgggcc gagaaatcgc
      481 ccccctgctg ctcgataaag ccgcggatcg ccgtcaccag catctcgcgg cccacgacga
      541 cgaccgccat ccagggcgtg atgccgctcc cttgctcggc cgcgagaaag ataaacgtgc
      601 cgcagatgat gaccttatcg acgaacggat cgaagatccg gccgagcttg gtcacttgct
      661 ggtacttgcg ggcataccag ccgtcgatcc agtcggtccc cgcggcgatc acgaacacga
      721 ccaccgccgc cgcgtattgc tccagcggga tcagcacgaa caccacgatc gacagcacga
      781 accgcgcggc cgtgaggacg ttcgggacgt tgaagatcgc agtggatgca gtcttcgttg
      841 atgccacgat acaagcttcc caatcttggg cagagccaaa tgcctaaggg cgatatgcca
      901 cccttgacac ggtggctcgt cgatcgccgt gatcgacttc ataaaccaca accagcggcg
      961 gcgcgacaat cagccgcgtt ccctcaaaat agctctcgcc ggctgccgac tgttgcccga
     1021 gaaactgatc gatcttggta acggcccggc tcacaccgtc cttgtcctca gccgcgagcc
     1081 agatctcggt caaccgatcg agcgccgacg gcttccagat gacggtgtat ttcatggaac
     1141 ctggattcaa gcttccgatt caagcttccg caggtgttcc agcacctcag ccgttgtgta
     1201 gccgcccgtt tccgcccgtg ccttcgcaat ttcctctcgt gtgaactgct gtctggccca
     1261 ctcatagtcc tcgggagtcg cctcgcgcat cggcgtgaac atcccaacta actgcccgtc
     1321 ctccgtgcgg acttcgacgg tcgtttgcgg ctgtttcagc tttgccgccg tggaggcgtc
     1381 gatcaggaca gtgctcatga cattctcctc gtaggtcgaa ctcccaatta taccttgccc
     1441 agcgccagca gcggagcgct cacgacctgc tccctccccc gcggcttgcc ggcggcgacg
     1501 ccaaccaggt cgtagtcctg cgtggcgacg atctcgacgg gcacgatgtc gccggcccgc
     1561 agcttgtgct ggtcggccgt gacgaacacc agcccatcga cgtccggcgc gtcggccgtg
     1621 ctgcggccga gccacacgcc ccgttcgccc tccacgggct ggtcgaggat cacgtcgcgc
     1681 gtctggccca cttgccgctg gttgtagtcg aacgcgattt gctgctgaag ctgcatcagc
     1741 cgctcgcggc gggcttccat cacgccctct ggcacgggat tcggcagatt ggccgccggc
     1801 gtgtcggatt caaaggaata cgtaaagacg cccatccgct cgaacttgta tttttcgatg
     1861 aattcggcca actgatcgac gtcagcctcc gtttcgccgg gaaagccggt gatgaatgtc
     1921 gtccgcagcg ccagcccctc gatccgcgac cgcagccggt cgagcaattc ctccgtttcc
     1981 gcgcggctca cgcggcgggc catccgcttc agcatggtat cgctggcatg ttgcagcggg
     2041 atgtcgatat acggcagaat cttgcggctc tcggcgatcg tatggatgag ctcgtcgtcg
     2101 atgtacatcg gatagaagta catcaggcga atccagtcga tcccgcggac ttcctccagc
     2161 ttccgcagca gctccgtcag ccgcgtctcg ccatacagat cgaggccgta gtatgtcgtg
     2221 tcctgcgcga cgacgatcag ctcccgcacg ccgtcggctg cgagctgctg cgcctcggcg
     2281 atgacctcct cgatcggttt gctggcgtgc ttgccgcgca tcttgggtat cgcgcagaac
     2341 gtgcagagcc ggtcgcagcc ttcggaaatc ttc
//
LOCUS       DASUGB010001215         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1387677, whole genome shotgun sequence.
ACCESSION   DASUGB010001215 DASUGB010000000
VERSION     DASUGB010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387677"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFV87_23005"
     CDS             complement(<1..604)
                     /locus_tag="VFV87_23005"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX5106716.1"
                     /translation="MPGHAFAIPAGTPAITRDLRRAGGVYYTPAEIVHELVSRTIGDL
                     QPKADGPPRILDPACGAGAMLVGAKRRGVELYGVDCDRPAVNAARRECGAEVWCADAL
                     FDRRLDALAPFDAVVANPPYVNIRQLANSRSRAYIERLRARFRTARGNFDLYVVFFER
                     ALELLGTGGRCGMIVPNKWATLDYARPLRKMLLTETTIEHV"
     gene            815..1285
                     /locus_tag="VFV87_23010"
     CDS             815..1285
                     /locus_tag="VFV87_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106717.1"
                     /translation="MSPLLPLAGTAALSLARETVGAIGNGLSFATELLRQDRRAASPT
                     EPEPNAVATSDASARFEEALCSFVARLKQRLAAAGVGLNDPLELTDDGLGGIQVAGDR
                     FDRAEIEQLLAADRQLVSEFQRLAEQQQALAADAAQMSSFALRITSGEAEVVAG"
     gene            complement(1342..>2316)
                     /gene="hemG"
                     /locus_tag="VFV87_23015"
     CDS             complement(1342..>2316)
                     /gene="hemG"
                     /locus_tag="VFV87_23015"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX5106718.1"
                     /translation="ATLPQFVEMERRHGSLIRGMRVREVGGQTSEVRESGPNHVPASL
                     ARRFSEDNQYGAGARYGQFVAPRDGMQRLVEALARRLPPTTIRLNAPVEQVQFVGSPK
                     FDGGWRLRVVGDSTEQYFDRLIVAAPAAVSSRLLNGVDPTLADLVGRIPYAGCAVALL
                     GIRRDQLVRRIDGFGFVVPAIEKRRIIAGSLASLKFPGRAPEGKLLLRIFIGGALQPQ
                     LVDLSDDEIRRLVLAELSELVGLTGEPEFFDVARWRGMMPQYHVGHLDLVRQIEERAV
                     AIPNLALAGNAYRGVGVPFCIRSGELAAERLVASAERGAGSAERGQTT"
BASE COUNT          401 a          803 c          741 g          371 t
ORIGIN      
        1 tcacgtgctc gatcgtggtt tcggtcagca gcatttttcg caaaggccgg gcgtagtcga
       61 gcgtggccca tttgttcggc acgatcatcc cgcagcggcc tcccgtcccc agcaactcca
      121 gcgcccgctc gaagaacacg acgtacaaat caaagttgcc ccgcgccgtg cgaaaccgcg
      181 cccgcagccg ctcgatatag gctcgcgacc ggctgttggc gagttgccgg atgttgacgt
      241 acgggggatt ggccacgacg gcatcgaagg gggccaatgc gtcaagtcgg cggtcgaaca
      301 gggcatcggc gcaccacact tctgcgccgc attcgcggcg cgcggcgttg acggccggcc
      361 ggtcgcaatc cacgccatac agctcgacgc cgcggcgctt ggctccgacc agcatcgcac
      421 ctgcgccgca agcggggtcg aggatccgcg gcggcccatc ggcctttggc tgtagatcgc
      481 ctattgtgcg acttaccagt tcgtggacga tttccgcggg cgtgtagtaa acgccgcccg
      541 cccgccgaag gtcccgcgtg attgccgggg tgccagcggg gatagcaaag gcgtgaccgg
      601 gcaagacgcg gctcccgcga acggcgcgaa gtaatgggct gcggatcgta gtcggcctgc
      661 ctgcggccgc caaccgcaac atcccgcccg agcagcccga gctttggctg aagtcctgcc
      721 gcccgcccgt cgataatgga ccgccggccc gcgcgccggg cggcgctcgc tagcaagcga
      781 tcgccggcat tccaacaggc tcatcagcga acgtatgtcg cctctgctcc ctttagccgg
      841 aaccgccgcc ctctcgctgg cccgcgaaac ggtcggggcg atcggcaatg ggctgtcgtt
      901 cgccacagaa ttgctgcggc aggaccgccg cgcggccagc cccaccgagc ctgaaccaaa
      961 tgccgtggcc acctcggacg ccagcgcccg ttttgaggaa gccttgtgct cgtttgtcgc
     1021 ccggttgaag cagcgcctgg cggcggcggg ggtcggcctc aacgacccgc tggagctgac
     1081 cgacgacggg ctgggcggga tccaggtggc aggcgaccgc tttgatcggg cggaaatcga
     1141 gcagcttttg gcagcagacc gccagctggt gagcgaattc cagcggctgg ccgagcagca
     1201 gcaggcattg gcggccgacg cggcgcaaat gagcagtttt gcgctgcgaa tcaccagcgg
     1261 cgaggcggaa gtcgtcgcgg gctagacacg tgcgcgaccg ctggtcgcgg ctatgcatcg
     1321 gtgcgcgacc gctggtcgcg actatgtagt ttgtcctcgc tccgcactcc ccgctccgcg
     1381 ctccgcgctg gctaccagcc gctccgccgc cagctcgccg ctgcgaatgc aaaacggcac
     1441 acccacgccc cggtatgcat tgcctgccag ggccagattg ggaatcgcca ccgcccgctc
     1501 ttcgatttgg cgcaccagat ccaaatgccc tacgtgatat tgcggcatca tgccccgcca
     1561 gcgggccacg tcgaaaaact ccggctcccc ggtcaggccc accagctcgc tcagctccgc
     1621 cagaaccagc cggcggattt cgtcgtcgga caggtccacc aattgcggct ggagcgcgcc
     1681 gccaataaat atgcgcagca gcagcttccc ctcgggggcc cgtccgggga atttgaggct
     1741 ggccaggctg ccggcaatga tccgtcgctt ttcgatggcc ggcacgacga aaccgaagcc
     1801 gtcgatccgc cgcacgagct gatcgcggcg aattcccagc aaggcaaccg cacagccggc
     1861 gtagggaatg cggccgacca agtcagcgag cgtgggatcg acaccgttca acagccgcga
     1921 gctgacagcc gccggagcgg caacgataag ccggtcgaag tactgttcgg tggagtcgcc
     1981 gacaacccga agcctccagc cgccgtcgaa tttcggcgag ccgacgaact gcacttgctc
     2041 caccggcgca ttcaagcgaa tcgttgtcgg cggcaaccga cgggccagcg cttcaaccag
     2101 ccgctgcatt ccgtcccgcg gggcgacgaa ttggccgtag cgagcgccgg cgccgtactg
     2161 gttatcttcg ctgaaccgcc tcgcaagcga cgcggggaca tggttggggc ctgattctct
     2221 gacttctgac gtctgacctc cgacctccct gactcgcatt ccccggatca ggcttccatg
     2281 ccgtcgctcc atctcgacaa actgaggcag cgtggc
//
LOCUS       DASUGB010001216         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1525172, whole genome shotgun sequence.
ACCESSION   DASUGB010001216 DASUGB010000000
VERSION     DASUGB010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525172"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="VFV87_23020"
     CDS             complement(<1..2352)
                     /locus_tag="VFV87_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX5106719.1"
                     /translation="MTIEMNNGLPARQGLYDPANEKDSCGVGFIAQIKGVRSHSIVRD
                     AAEALRAMDHRGACGCEANTGDGAGILTAIPDELMRAEAQRLFGVELPEIGRYAVAQV
                     FLPRDEAERAICKETLEKYVQRQGQKLLGWRSVPTDAKGADIGASAAATEPVVEQLFV
                     AASNEIDRTAFCRQLYLIRKQAFHALNRLGAERHGGRSLQQRGMYYVCSFSSRIIVYK
                     GQLTSKQVVPYYPDLADPRYTSHLAMVHSRFSTNTFPSWERAQPMRFMSHNGEINTLR
                     GNVNWMAAREGMLKSELFGDDLKKLFPITDMSTSDSGVFDNVLELLLLGGRSLPEAVM
                     MMIPEAWQNHESMPQEKQDFYEYHSCLMEPWDGPASVAFCDGRYIGAVLDRNGLRPSR
                     FYITTDDRVIMASEVGVLPVDPKIVKHKGRLQPGRMFLVDFEQGRLIPDEELKKEVAT
                     RRPYGEWVRRQRIELKDLPLADQAHGFEPETLLARMQAFGYSTETMQFMLLPLVHEKR
                     DPVGSMGNDSALACLSDQPRMLYDYFKQLFAQVTNPPIDSIREEVIMSLECYVGPEGN
                     LLATTEEQANRLLLPHPILTNEEVSALEDLDHRGWKSKLIDITYDRSEGEAGLVNRLD
                     RICREAEQAIDRGYSLVVLSDRGTSAERVPLSSLLAVGAVHHHLIRQAKRTQIGIILE
                     SGEAREVHHHCLLVGYGADAINPYLAFEALWQAERVGLLPSKYSDDDIVHAYQKAVAK
                     GMLKVMSKMGISTLQSYKGAQIFEAVGLNEAVVDKCFAGTASRI"
     gene            complement(2420..3469)
                     /locus_tag="VFV87_23025"
     CDS             complement(2420..3469)
                     /locus_tag="VFV87_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5106720.1"
                     /translation="MHLKSLKVFCDVVGRRSFSRAAAENGISQSGASQIVNGLEEHLG
                     VQLIDRTKRPFVLTPEGEVYYEGCRKLVQRYFALEEDVKTLHEEVAGRVSVASIYSIG
                     LSHMNRLVQRFLQAFPKANVRVQYQHPNRVYELVESDQVDLGLVSFPRSSRAIKASAW
                     RAEPMVVVVAPEHPLAGRESVKLEELRGAEMVAFDADLEIRHEIDRALAAAGVEIRVV
                     MEFDNTETIKRAVEIDAGISLLPEPTVAREVAAGALVARPLAGIELKRPIGIIQRRGK
                     ELGKTARRFMQLLLKQPATAREIAELDAADSVALDDAAQEELLECEHSSAARDSLPAP
                     VKSAPLAEPATRAAS"
     gene            complement(3686..>4099)
                     /locus_tag="VFV87_23030"
     CDS             complement(3686..>4099)
                     /locus_tag="VFV87_23030"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5106721.1"
                     /translation="NGVDDILSKEEWGGGTGRGISLRLEDRRPEVNFGSGPEWLTLRA
                     PQPAETGKWMHLVGVYDGEHQALFIDGVEVSSMACSKAISVSPQPIRIGRGPFAQERR
                     FKGIIDEVALFDLALTAEEIRLTHQLGSGGKPLSK"
BASE COUNT          737 a         1343 c         1218 g          801 t
ORIGIN      
        1 aatgcggctg gcagtcccgg caaagcactt atcaacgacc gcttcattca gcccaactgc
       61 ctcgaagatc tgggctcctt tgtacgactg cagcgtggag atgcccatct tgctcatcac
      121 cttgagcatc cccttggcga ccgctttctg atacgcgtgg acgatgtcgt cgtcgctgta
      181 cttgctgggc aagaggccga cgcgctcggc ctgccagagg gcctcgaacg ccagataggg
      241 attgatcgca tcggccccgt agccgacgag gaggcagtgg tgatgcacct cgcgggcttc
      301 gccggattcg agaatgatcc cgatctgcgt ccgctttgcc tgacgaatga gatgatggtg
      361 gacggccccc accgcgagca gcgagctgag cggcacgcgc tcggccgaag tgccgcgatc
      421 ggacaggacg acgagcgaat agcccctgtc gatcgcctgc tcggcttctc ggcagatgcg
      481 gtccagccgg ttgaccaggc ccgcttcgcc ttcgctgcgg tcgtaggtga tgtcgatcag
      541 cttgctcttc cagccgcgat ggtcgagatc ctccagcgcc gaaacctcct cgttcgtcag
      601 gatcggatgc ggcaacagca ggcggttggc ctgttcctcg gtcgtggcca gcaggttccc
      661 ctccggcccg acgtagcact ccagcgacat gatcacttcc tcgcggatcg aatcgatcgg
      721 cgggttcgtg acctgggcga agagctgctt gaaatagtcg tagagcatcc gcggctggtc
      781 gctcaggcag gccagggccg aatcgttccc catcgagcca accgggtcgc gcttttcgtg
      841 caccagcggc agcagcatga actgcatcgt ctcggtcgag tagccgaagg cctgcatccg
      901 cgccagcagc gtttccggct caaagccatg ggcctggtcg gccagcggca agtccttcag
      961 ctcgatccgc tggcggcgga cccactcgcc gtaaggacgc cgcgtcgcga cttccttctt
     1021 cagctcctca tccggaatca accgcccctg ctcgaaatcg accaggaaca tccgccccgg
     1081 ctgcaggcgt cccttgtgct tgacgatctt cggatcgacc ggcagcacgc ctacttcgct
     1141 ggccatgatc acgcggtcgt cggtcgtgat atagaaccgg ctggggcgca ggccgttgcg
     1201 atcgagcacc gcgccgatat atcgcccatc gcagaacgcg accgaagccg gcccgtccca
     1261 cggctccatc aggcaggagt ggtactcgta aaaatcctgc ttctcctgtg gcatcgattc
     1321 gtggttctgc cacgcctcgg gaatcatcat catcaccgct tcgggaagcg agcggccgcc
     1381 gagcaagagc aactccagca cattatcgaa cacgcccgaa tcgctcgtgc tcatgtcggt
     1441 gatcggaaac agcttcttca gatcgtcgcc gaacagttcc gatttgagca tcccttcgcg
     1501 agccgccatc cagttcacgt tcccccgcag tgtgttgatc tcaccgttgt gcgacatgaa
     1561 ccgcatcggc tgggcccgct cccacgaggg gaacgtgttg gtgctgaacc gcgaatgcac
     1621 catcgccagg tggctggtgt agcgcgggtc tgccaggtca gggtaatacg gtacaacttg
     1681 cttgctcgtg agctgccctt tatagacgat gatccgcgag ctgaagctgc agacgtagta
     1741 catgccccgc tgctgcaggg aacgcccccc gtggcgttcc gctcccaggc gattcagtgc
     1801 atgaaacgcc tgcttgcgga tcagataaag ctgccggcaa aacgcggtgc ggtcgatttc
     1861 gttgctggcc gcgacgaaga gttgctcgac gaccggctcg gtggccgcgg cgctggcgcc
     1921 gatgtcggcc cctttggcgt cagtcggcac gctccgccag ccgagaagct tctgtccctg
     1981 gcgctggacg tacttttcga gcgtttcctt gcagatggcc cgctcggctt cgtctcgcgg
     2041 cagaaatacc tgcgccaccg catagcggcc gatctccggc aattccacgc cgaacagccg
     2101 ctgggcctcg gcccgcatca attcatcggg aatggccgtg agaatgcccg cgccgtcgcc
     2161 cgtgttggct tcgcagccgc acgcgccgcg gtggtccatc gcgcgcaggg cctcggcggc
     2221 gtctcggacg atgctgtggc tccgcacgcc cttgatctgg gcgatgaaac ccacgccgca
     2281 gctgtccttt tcgttcgccg gatcgtaaag accctgccgg gcaggcagtc cgttgttcat
     2341 ttcgatcgtc atcttcaggc atcccgagac aagggagtgc cgcccgcgcg ccgagagttc
     2401 agcgaggcgg cgtggaacct tacgacgcgg cccgcgtcgc cggctcggcc agcggcgcgg
     2461 atttgacggg cgccggcagc gaatcgcggg ccgccgacga gtgttcgcat tcgagcagct
     2521 cttcctgcgc agcatcgtcc agcgccacag aatcggccgc atccagctcg gcgatctcgc
     2581 gggccgtcgc cggctgcttg agcagaagct gcataaagcg gcgggctgtc ttgcccagtt
     2641 ccttgccccg gcgctggatg atgccgatcg ggcgtttgag ctcgattccc gccagcggcc
     2701 gggcgacgag cgcccctgcc gccacttcgc gggccaccgt cggttcgggc aacaggctga
     2761 tgccggcgtc gatctcgact gcccgcttga tcgtttcggt attgtcgaat tccatcacca
     2821 cgcggatttc gacccccgcg gcggccagcg cccggtcaat ctcgtggcga atctccaggt
     2881 cggcgtcgaa ggccaccatc tccgccccgc gcaattcttc cagcttcaca ctctcgcgcc
     2941 ccgccagggg atgttccggg gcgacgacca ccaccatcgg ctctgcccgc cacgcgctgg
     3001 ctttgatcgc ccggctggag cggggaaagc tgacgaggcc cagatcgacc tggtccgact
     3061 cgaccagctc ataaacgcga ttggggtgct gatactgcac gcggacgttg gccttgggaa
     3121 aggcctgcaa gaaccgctgc accaggcggt tcatgtgcga caggccgatc gaatagatcg
     3181 aggccacgct gacccgcccg gcaacctcct catgcaatgt cttgacgtcc tcctccagag
     3241 cgaaatatcg ctggacgagc ttacggcatc cctcgtaata aacctcaccc tcgggggtca
     3301 gcacgaacgg ccgcttggtc cggtcgatga gctgcacccc caggtgctcc tccaggccgt
     3361 tgacgatctg gctggcccct gactgcgaaa tcccgttctc agccgcagcc cgcgaaaagc
     3421 tgcgtctgcc gacgacatcg cagaagactt tgagggactt gaggtgcatt tgctgaatta
     3481 gccacgataa tactgcatcg ccgaattatg agcggcgagc atcgtagagt atttggtaaa
     3541 atgcaatttg tcaattaccg ttcctggaaa atcagaccac cggcgaatta gaaccttgcc
     3601 gcagcgtcgc tcggccggtt aggaaaggcg ccttctaccg tttgggattc taaatgccgt
     3661 gctagaaagc gtttagagcg ctagatcact tcgaaagcgg tttcccgccg ctacctaatt
     3721 gatgggtcag gcgaatctct tcggcggtca gcgccaaatc aaagagggcg acttcatcga
     3781 taattccttt aaaacggcgt tcctgtgcaa acggtccacg cccaattcga atgggttgcg
     3841 gtgagacgga gattgccttg gagcacgcca tggaagagac ctccacacca tcgataaaca
     3901 gtgcctggtg ttcgccatca tagactccta ccaggtgcat ccactttccc gtttccgcag
     3961 gctgaggagc acgaagggtc agccattcag gtccgctgcc gaaattcacc tcgggtcgcc
     4021 ggtcttcgag tcgcagcgag atcccgcgac cggtgccgcc gccccattct tccttactga
     4081 gaatgtcgtc cactccatt
//
LOCUS       DASUGB010001217         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1525183, whole genome shotgun sequence.
ACCESSION   DASUGB010001217 DASUGB010000000
VERSION     DASUGB010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525183"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFV87_23035"
     CDS             <1..615
                     /locus_tag="VFV87_23035"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX5106722.1"
                     /translation="GLRVPFIVSWPGVVEAGTKADAMVSWIDLLPTLVEAAGGQPPQA
                     GSGVGEIDGRSFLAVLSGKSREHRDRIFGTHSGDREMNVYPIRSLRQGNWKYIWNLHP
                     EFQHTTHVDRAQAEDEVGFFRSWERAAAAGDKHASAAIERYRKRPADELYDLDTDPLE
                     QHNLAGDPAHAEMVKALHATLEAWMNEQGDQQTVFNNPVLLPRP"
     gene            836..2896
                     /locus_tag="VFV87_23040"
     CDS             836..2896
                     /locus_tag="VFV87_23040"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HEX5106723.1"
                     /translation="MRRHAIRRDPEVEESPVPASNAATSEGDSNDLAEGTQPALTKSG
                     KPQVVSNRPSDTAEVEAKAAAKAGASSDRVGQVAPASKKVAPEPKGPIRVVIDNAKPA
                     EPLTGEQRRLRSIQNLQSIGRALGAHVQRTGALPTVIRDEQGKPLLSWRVALLPDLGY
                     GELYRQFHLDQAWNHPWNEKLLAQIPPEFQSPERFDRRTNYLGLAGSGQAFAGPRGAV
                     PSAMEDGSENTVLVIEVDDGRAANWTAPDDFVPATGSSRSGLGRLRGDGVFALLGNGR
                     VVRLGPDLPDSQFLALFSPDGGEQVSTEDLSEPTRQAAEIAVAPAVEPFAASVPESPV
                     GKPADVATTPAASSPAAPPEIALPGEPSLSSNPDLLPVPPEEALAAARALFRELYGKQ
                     YDQARSWDDKAKLAKQLLADVAKVKANPAHYYELLRIVRDVAAAGGDADSAHQAAEYL
                     TERFQIDGLAMRLKLLEDLSKSPRKTETVETLRKIARELLLDAFDADRFDVAIPAYDR
                     LVEFTRLKGERGELSRLTQSKQPLEAARQAYVAACAAVETLKANPDDGAASETLGKYL
                     CFVKNRWDAGLPYLVRAAEVQLRVVATIDRETARSPHDTLSLADHYWEMANDYKQPFS
                     RGLHLRSALCYQTALDQLPDGLEKLKAQKRIDEVGEIYSPEEVARALNRPVRAAARME
                     DAED"
     gene            3039..>3983
                     /locus_tag="VFV87_23045"
     CDS             3039..>3983
                     /locus_tag="VFV87_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106724.1"
                     /translation="MPAAPTELKLIFCAVNEGVGRYFWKQLVELVSSGQFEQEIPRYK
                     FDLVRCATEEEALARLRQYAAAADKPALILLSDGLVEAAGPISTIDSWLPTSWAKEVG
                     DVLGARGIKIAITDESRQVTDIDRTLVHKAKPAIILEALKLAAEKLTYMARPEKRAAS
                     PAIEVRLIRRQHELLDYFRLRHRIYRLMGYLRADIESAPSQMEMDWCDSIALHIAAYE
                     RLRGGREILAGTARVVVASSATPQKHGDLLAMYKHWVENLAGGDPVLGQAIEQTLELE
                     LPIFHSQNLARVLKQAVEKEEVYGELSRVIVAEDYRGGG"
BASE COUNT          781 a         1208 c         1262 g          732 t
ORIGIN      
        1 gggttgcgcg ttccgttcat cgtttcgtgg ccgggcgtgg tcgaggcggg aacgaaagca
       61 gacgcgatgg tgagttggat cgacttgttg ccgacgctcg ttgaggctgc tggcggccag
      121 ccgcctcaag ccggcagcgg ggtgggggaa atcgatggcc ggtctttcct ggccgtgctg
      181 agcggcaaat cgcgcgaaca ccgggatcgg atcttcggca cgcactccgg cgaccgcgag
      241 atgaacgtct atccgatccg cagcctgcgc cagggcaact ggaagtacat ctggaacctg
      301 cacccggagt ttcagcacac gacccacgtc gatcgggccc aggcggagga cgaagtgggc
      361 tttttccgct cctgggaaag ggctgccgca gccggcgaca agcacgcctc ggccgcgatc
      421 gagcgctacc gcaagcgacc cgccgatgaa ctctacgatc tggataccga tccactcgag
      481 cagcataatc tcgcgggtga tcctgctcat gccgaaatgg tcaaggcgct gcacgcaacc
      541 ctagaagcct ggatgaacga gcaaggagat cagcaaacgg tcttcaacaa tccagtcctt
      601 ctaccgcggc catgagtctt cgagagatga gagatggttg tcttcaccga agctttacca
      661 tttcttcatg ctttggcctg acaatgtccg tgcgttttgg gcacgttagc cctcccagtg
      721 ctggatcact tccacgccct caaatcgcac tatgcgcaac gcggcactga gattctgttt
      781 tgcgagcgtt tgcttctctg tagtgctaac tggctgtgga ggcggccagg cggacatgcg
      841 gcgccatgca atcaggcgcg atccagaagt agaagagtca cctgtacccg cttccaacgc
      901 cgccacatcc gagggcgaca gcaacgatct cgcggaggga actcagcccg cacttaccaa
      961 atctggcaag ccgcaggtcg tatcgaaccg accatctgac accgcggagg ttgaggcgaa
     1021 ggccgcggca aaagctgggg cgtcaagcga tcgggttgga caagtcgcac ccgcgtcaaa
     1081 aaaggtagcc cccgaaccca agggtccgat tcgagtcgtc atcgataacg ccaagccggc
     1141 tgagccgctg accggcgagc agcggcggct gcgcagcatt cagaatctcc agtccatcgg
     1201 ccgggcgctt ggcgcccacg tccagcgtac gggggcgctt ccgactgtga ttcgcgacga
     1261 gcaaggcaag ccactcctga gttggcgggt cgcgctgttg ccagatcttg gctacggcga
     1321 attgtatcgc cagttccatc tcgatcaggc gtggaaccat ccatggaatg aaaagctgct
     1381 cgcgcagatt ccacctgagt tccaatctcc cgagcggttt gaccgcagga cgaactattt
     1441 gggcctggct ggcagcggcc aggctttcgc cggtccgcgc ggggcggttc ctagcgccat
     1501 ggaagacggc agcgaaaaca cggtgctcgt gatcgaggtg gacgatggcc gggccgcaaa
     1561 ctggacggcg cctgacgact ttgtcccggc gacgggttcc tctcggagcg ggctcggccg
     1621 attgcgcggc gacggagtgt ttgcactgtt gggcaacggc cgtgtcgtgc gtttggggcc
     1681 cgatcttccg gattctcagt ttcttgcctt gttttctccg gatggaggag aacaggtatc
     1741 gacagaggat ttatcggagc ccacgcggca ggccgccgag atcgctgtgg cgcctgccgt
     1801 cgagccattc gcagccagcg ttcctgaatc gcctgttggc aagcctgccg atgtagcgac
     1861 gactcccgcc gcaagcagtc cagcggcgcc gccggagatc gccttgcccg gtgagccgtc
     1921 cctttcgtct aatccagatc tgctccccgt gccgccggag gaggcgcttg ccgccgcgcg
     1981 cgcgctgttc cgcgagctgt acggcaagca atacgatcaa gcccgttcgt gggacgataa
     2041 ggccaagctc gccaagcagt tgctggcgga cgtggccaag gtgaaagcga acccggcgca
     2101 ctactatgag ctgctcagga tcgttcgcga tgtggcagca gccggcggcg atgccgactc
     2161 cgcccatcag gcagccgaat atctgacgga gcggttccag atcgacggtc tggcaatgcg
     2221 cctcaagctg ttggaggact tgagcaaatc accgcggaag acggaaaccg ttgaaacgct
     2281 gcgcaaaata gcgcgggaac tgctcttgga cgcgttcgat gcagatcggt tcgacgtggc
     2341 tatcccggcg tacgaccgac tggtcgagtt cacgcgctta aagggcgagc ggggagagct
     2401 gagccgactc acccagagca aacagcctct cgaagccgcc aggcaagcct atgtcgcggc
     2461 ctgtgcggcc gtggaaacac tcaaagccaa cccggacgac ggcgcggcaa gtgaaacgct
     2521 cgggaagtac ctctgctttg tgaagaatcg ctgggacgcg gggctgcctt atctcgtgcg
     2581 ggcggctgaa gtccagttgc gggtcgttgc caccatcgat agggagacgg cgcgttcacc
     2641 gcacgatacg ctgtcgctgg ccgatcacta ctgggaaatg gccaacgatt acaagcagcc
     2701 gttctcgcgc ggactgcatc tgcggtccgc cctctgctac cagacggctc tcgatcagct
     2761 tccagatggg ctggaaaagc tcaaagccca aaagcggatc gacgaggtgg gcgaaatcta
     2821 cagtcctgaa gaagtcgctc gggccttgaa ccgtccggtt cgagcagccg cgcgtatgga
     2881 ggatgctgaa gattgacgtt cgtcccagaa ggtgataaac tccccccctg tcgttcgaca
     2941 cgcgtctttc cctcgttcgc ccctctttga tggctcgccg gcgctcggtt gcgccggcag
     3001 cggcgcggcc aaatgtcccc cagaatggga gcgctatcat gcctgccgcc ccaactgaat
     3061 tgaagctcat cttctgcgcc gtcaatgaag gcgtgggccg ttacttctgg aaacagctcg
     3121 tcgagctggt ttccagcggc caattcgagc aggaaattcc gcgttacaag ttcgatctcg
     3181 tacgctgtgc cacagaggag gaagcgctgg cccgcctgcg gcagtatgcg gctgcggcgg
     3241 acaagcccgc gctgattctc ctttccgatg gactggtcga agcggccgga cccatctcga
     3301 ccatcgatag ctggctgccg acgtcgtggg cgaaagaggt gggcgacgtg ctcggtgcgc
     3361 gggggatcaa gatcgccatc accgacgagt cccgccaagt cacggacatc gaccgcacgc
     3421 tcgtccataa ggccaagcca gcaatcattc tggaggccct caagctcgcc gccgagaaac
     3481 tcacctatat ggcgcggcct gaaaagcggg ccgcttcgcc ggcgatcgaa gtgcgcctga
     3541 tccgccggca gcacgagttg ctcgattact ttcgcctgcg tcatcgcatc tacagattga
     3601 tgggctatct gcgggcagat attgagagcg ctccatcgca gatggaaatg gactggtgcg
     3661 actcgatcgc cctgcacatt gccgcgtacg agcgtctgcg gggcggccgc gaaatcctgg
     3721 ccggcacggc gcgagtcgtg gttgccagtt ccgcgactcc ccaaaagcac ggtgatctgc
     3781 ttgcgatgta taagcactgg gtcgaaaacc tggccggggg cgatccggtg ctcggtcagg
     3841 cgatcgaaca gacgctcgaa ttggagctgc cgatctttca ctcgcagaat ctggcccgtg
     3901 tgctcaagca ggcagtcgaa aaggaagagg tctatggcga gctcagccgg gtgatcgtcg
     3961 ccgaggatta ccgcggcggc ggg
//
LOCUS       DASUGB010001218         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1525224, whole genome shotgun sequence.
ACCESSION   DASUGB010001218 DASUGB010000000
VERSION     DASUGB010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525224"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFV87_23050"
     CDS             <1..231
                     /locus_tag="VFV87_23050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106725.1"
                     /translation="AAPPRTAAANVETPAGPRLATIEHEPEESEELEPAKVLRRRFQS
                     TGPDLRAELQEIVKENPDAAANILRAWIGEAA"
     gene            252..1319
                     /locus_tag="VFV87_23055"
     CDS             252..1319
                     /locus_tag="VFV87_23055"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HEX5106726.1"
                     /translation="MLPAALRKAAILISGLDDRAADALLEEMGPEQAAKVRSAIMQLD
                     EIDAAEEQRVLAEFFERQNAPARGSAKDVEADGVVLELSSPEIPPESRAVPAAAESPA
                     TTTDEFLAAAEAEQLANVLNDEHPQTVAVIVAHLAPQRAAAVLERLPANLATEALERM
                     ASLGPVDRSVLDEIERGLERRLVWRFGAMVSGGQSARRLGEVLDAMDFRQRERILLQL
                     AERNASLAGSIPNSPSKQTSTSESQFTQFRYRLERPNAVPERESRRFKFRDLVRLHDD
                     LLRDVFAAAEPTVVLLALTGAEEPLLKRVLRQLPSQDAALLRERLNHPGPLRLREVEQ
                     AQQQLAATAVRLAGSDKEPLV"
     gene            1413..2066
                     /locus_tag="VFV87_23060"
     CDS             1413..2066
                     /locus_tag="VFV87_23060"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX5106727.1"
                     /translation="MAGIIKAAGSRETAPHGAQAFQFADVGQAYVGSVRSESAKIVAQ
                     ARREAAQIKARAEEEGKQAAIQAVRAALRSQLDQQLASALKAMQQAVKEIADSRQAWQ
                     RHWEERAVKLGAAIAARVIRRELDRTPDITLTWVREALQLAAGSGQVVLRLHPQDLAT
                     VGDRIDAVAGELHRLGPLQVVADEAISPGGCKVETVFGSIDQQIEAQLARLEEELLG"
     gene            2075..3436
                     /locus_tag="VFV87_23065"
     CDS             2075..3436
                     /locus_tag="VFV87_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX5106728.1"
                     /translation="MIAALEELETMLPTAMVGSVVRTEGLAAAVAGLPAPVGAVVEIE
                     RAGASPVEAEVIGFRDDVAIVYPLGEMTGVRHGNQVRLARTRRLLSVGPELLGRVIDA
                     RGRTIDGRPQPVRSARVPLWGKTPPATDRPRIRTPLSTGVRAIDGLLTCGQGQRIGIF
                     SGSGVGKSVTLGMMARYTSADVTVVGLIGERGREVNDFLERDLGPEGRARSVVIVATS
                     DQPALTRVQAALTATAVAEHFRDRGQNVLLIMDSVTRFALSQREIGLAAGEPPTTRGY
                     PPSVFALLPRLVERAGRTPQGSITAFYSVLVEGDDTNEPVSDTLRGLLDGHIVLSRKL
                     AAQAHWPAIDVLESISRLFPDIVSPEQRAAAQTIRELLAAYRDHEDLISIGAYRSGAN
                     PNVDCALVMRDEIQRFLRQAIEEKSSVESAATALIELAQRCQAARKPALPPAPVQSLR
                     GKT"
     gene            3537..3995
                     /gene="fliJ"
                     /locus_tag="VFV87_23070"
     CDS             3537..3995
                     /gene="fliJ"
                     /locus_tag="VFV87_23070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX5106729.1"
                     /translation="MPPFRFRLETLLRLRIGERDERRADLAKALRAEAMLRDERERIG
                     IEQIEIQTAIRQRSAPGSADVDALLRVSRYQAVLKARDQQLEQQLTQVIAEVERRRLV
                     LAEADRQVRVLEKLRERQFADHRQRANRLEIKELDEVAATGHLRRAEVRA"
     gene            3992..4693
                     /locus_tag="VFV87_23075"
     CDS             3992..4693
                     /locus_tag="VFV87_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106730.1"
                     /translation="MKTLFNWLGTLLVAFSIGTVLSLAVLLGMLWWKGALTDDRLVGM
                     IAALQGIKPVPPASSTPNAAEAEQPSLDQIMQGRLRASLDLDLRESAIDKSLGDLRAV
                     ETQIKAERERLDLWKVDFDARLAKLENAATDAAILEVQRTLESMQPKQAKDQILKMLD
                     EPATNIDDPMRDVVTVLKSMADDKRKKLLAEFKTPEEAERLAEIIREVRLGLPDSEVI
                     RDTRNQLQQQLNPQR"
     gene            4702..>4963
                     /locus_tag="VFV87_23080"
     CDS             4702..>4963
                     /locus_tag="VFV87_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106731.1"
                     /translation="MNATASNSINGASSAIQPLKPATPQPALPFDALLQPAERLPPCY
                     ALEESECHAASNSKTSVDAAAQTEASDAGESPPADNQNVAEAE"
BASE COUNT          936 a         1604 c         1652 g          771 t
ORIGIN      
        1 gccgcgccgc cgcgaacggc tgccgcgaat gtcgaaacgc cggccggccc gcggctggcg
       61 acgatcgagc acgaacccga agagagcgag gagttggagc cggcaaaagt cctgcggagg
      121 cggttccaat cgaccggccc cgacctgcgg gccgaattgc aggaaatcgt caaggaaaat
      181 ccggacgcgg cggcgaatat cctccgcgcc tggattggag aagccgccta accactgcgt
      241 agaactgacc catgttgcca gcggccttac gaaaagcggc gatcctcatt tccgggctcg
      301 acgaccgcgc cgccgacgcc ttgctcgagg aaatgggtcc ggagcaagcc gccaaagtcc
      361 gcagcgcgat catgcagctc gatgagatcg acgccgcgga agagcagcgc gtgctcgcag
      421 aattctttga gcggcagaac gcgccagctc gtggcagcgc aaaagacgtg gaggcggacg
      481 gcgtcgtgtt ggagctctcg tcgcctgaaa tcccgcccga atcgcgggca gtaccggctg
      541 ccgcggaatc gccggccacc acgacggacg aattcctggc agcggcggaa gctgagcagc
      601 ttgccaacgt cctgaatgac gaacatccgc agaccgtcgc agtgatcgtc gcacacctcg
      661 cgcctcagcg ggccgctgcc gttttggagc gtttgccggc aaacctggca acagaggccc
      721 tggagcgaat ggcttcgctg ggtccggtcg atcggagcgt tcttgacgaa atcgagcggg
      781 gactggagcg gcggctggtt tggagattcg gagcgatggt cagcgggggt caatcggcca
      841 ggcgcctggg cgaggtgctg gacgcgatgg actttcgcca gcgcgagcgg attctgctgc
      901 agttggccga gcggaacgct tccctggccg gttcaatccc gaattcgccg agcaagcaga
      961 cgagcacaag cgagagccaa ttcactcaat tccgctatcg cttggagcgg ccgaatgctg
     1021 tgcccgagcg cgagtcgaga agattcaagt tccgcgatct ggtccggctc cacgacgatt
     1081 tgctgcggga cgtattcgct gccgctgagc cgacggttgt gctcctggcc ctgacgggag
     1141 cggaagagcc gctgctcaag cgcgtgctgc gtcagctccc ctcgcaagat gcggccctgc
     1201 tgcgggaacg tctgaatcat ccggggccgc tccgactacg cgaggtcgaa caggcacagc
     1261 aacaactggc ggcaaccgct gtcaggctcg cgggcagtga taaggaaccg ctcgtttagc
     1321 gggtagccga aggcgcctcg cctgtcatcc agcggcttcg cctgcggctc gccgttaaac
     1381 gatcccactg agaacaacca tcaacacacg ccatggccgg aatcatcaaa gccgcgggaa
     1441 gccgggaaac agctccgcac ggagcacagg catttcagtt tgctgatgtg ggacaagcct
     1501 acgtcggcag tgtgcgctcc gaatcggcga agatcgtggc ccaggcccgc cgcgaagcgg
     1561 cccaaatcaa ggctcgggcc gaggaagagg ggaagcaggc cgccattcaa gccgtccgcg
     1621 ccgcgctccg cagccagctc gaccagcagc tcgcttcggc cctcaaggcc atgcaacaag
     1681 cggtcaagga aatcgccgac tcgcgccagg cctggcagcg gcattgggag gagcgggccg
     1741 tcaagttagg cgccgccatc gccgcccgcg ttattcgccg cgagctcgac cgcacgcccg
     1801 acatcaccct cacgtgggtc cgcgaagccc tgcagcttgc cgccggcagc gggcaggtgg
     1861 tcctgcggct gcatccccaa gacctggcga cagtaggcga ccggatcgat gccgtcgcgg
     1921 gcgagctgca ccgcctgggc ccgctgcaag tcgtcgccga cgaagcgatt tcgcccggcg
     1981 ggtgcaaggt ggaaaccgtg ttcggctcga tcgatcaaca gatcgaggcg cagctcgccc
     2041 gtctcgagga ggagctgctg gggtaagcgc caccatgatc gccgcactcg aagagctcga
     2101 aacgatgctc ccgaccgcca tggtcggcag cgtcgtccgc acggaaggcc tggccgccgc
     2161 cgtcgcgggg ctgcctgcgc cggtcggcgc agtcgtcgaa atcgagcgcg ccggcgcgtc
     2221 gcctgtcgaa gccgaggtga tcggctttcg cgacgacgta gcgatcgtct atccgctggg
     2281 ggagatgacc ggcgtgcggc acggcaacca ggtgcgactg gcgcgcacgc ggcggctctt
     2341 gtccgtggga ccggagctcc tcggccgggt gatcgacgcc cgcggccgca cgatcgacgg
     2401 ccgcccgcag cccgtgcgaa gcgcccgggt gccactgtgg ggaaagacac cgccggcgac
     2461 cgaccgcccg cgaatccgca ctccgctttc gactggcgtg cgagccatcg atggtttgct
     2521 cacctgcggc caggggcagc ggatcggcat tttttccggc tccggcgtcg gcaagagcgt
     2581 cacgctgggg atgatggccc gctatacctc cgccgatgtg acggtcgtcg ggctgatcgg
     2641 tgagcggggc cgcgaagtca acgatttcct ggagcgcgat ctgggacctg aagggcgagc
     2701 ccgcagtgtt gtcatcgtgg ccaccagcga tcaaccggct ctcacgcggg tccaggctgc
     2761 cttgacggca acggccgtgg ccgagcattt tcgcgaccgc gggcagaacg tcctcctgat
     2821 catggattcg gtcacgcgct tcgccctctc gcagcgcgag atcggcctgg ccgccggcga
     2881 gccgccgacg acccgcggat acccacccag cgtgttcgcc ctgctgccgc ggctggtcga
     2941 gcgggcaggc cgcacgccgc aggggagtat taccgcgttt tattccgtgc tcgtcgaggg
     3001 ggacgacacg aacgagccgg tcagcgacac gctgcgcggc cttttggacg ggcacatcgt
     3061 tttgtcgcgc aagctcgccg cgcaggcgca ttggcccgcc attgacgtgc tggagagcat
     3121 cagccgactg tttcccgaca tcgtttctcc cgagcagcgt gcagcggccc agacgattcg
     3181 cgagctgttg gccgcctatc gcgatcacga ggacttgatt tcaatcggcg cgtatcgcag
     3241 cggcgccaat ccgaacgtgg attgcgccct ggtgatgcgc gacgagattc agcgatttct
     3301 gcggcaagcg atcgaggaga aatcctcggt cgaatccgct gccacggcgt taatcgagtt
     3361 ggcccagcgc tgccaggccg ctcgcaagcc ggcgctgccg cccgctcccg tgcagtcttt
     3421 acgaggaaag acctagtgtt cgctttgatc cgtagcagca cgcatatcaa cccgacgcgt
     3481 aagcgaggga caccctcgct tgcgcgtcgg gttagtatcc actagccact ccaaccatgc
     3541 ctccattccg cttccgcctc gaaacgttgc ttcgcctgcg aatcggcgag cgcgacgagc
     3601 gccgcgccga tctggcgaag gccctgcggg ccgaggcgat gctgcgcgac gagcgcgagc
     3661 ggatcgggat cgagcagatc gagattcaga cggccattcg ccaacgctcg gcgcccggca
     3721 gcgccgatgt ggacgctcta ctgcgcgtca gccgctatca agccgtgctg aaggcccgcg
     3781 atcagcagct cgagcagcag cttacccagg ttatcgctga ggtcgagcgc cgccggctgg
     3841 tgctggcgga ggcggaccgc caggttcgcg tgctggaaaa actgcgcgag cggcaattcg
     3901 cggaccatcg ccagcgggcg aatcggctgg aaatcaagga attggatgaa gtggccgcga
     3961 ccgggcacct gcggcgtgcg gaggtgcggg catgaaaacg ctgttcaact ggctcggcac
     4021 gctgctggtg gcgttctcga tcgggaccgt cctatccctt gcggttctcc tgggaatgct
     4081 gtggtggaaa ggagccctga ccgacgatcg cctggtcggc atgatcgctg cgctgcaggg
     4141 gatcaagccg gttcctcccg cttccagcac tcccaacgca gcggaagccg agcagccgtc
     4201 gctggaccag atcatgcagg gccggctgag ggcgagcctc gacctggacc tgcgggaaag
     4261 cgccatcgac aagtcgctgg gcgacctgcg ggcggtcgag acgcagatca aggccgaacg
     4321 cgagcggctc gatctgtgga aagttgattt cgatgcgcgg ctggcgaagc tggaaaacgc
     4381 ggccaccgac gcagcgattc tggaagtcca gcggaccctc gaatcgatgc aacccaagca
     4441 ggcgaaggac cagattctga agatgctcga cgagccggcc acgaatatcg acgacccgat
     4501 gcgcgacgta gtcacggtcc tcaagtcgat ggccgacgac aaacgaaaga agctcctagc
     4561 ggaattcaag acgcctgaag aagccgagcg gctggcggaa atcattcgcg aagtgcgtct
     4621 gggtctgccc gatagcgaag tgatccgcga cacacgcaac cagcttcagc agcaactcaa
     4681 tccacagcga tagtcttcgc aatgaatgcc actgcgtcca actcgatcaa cggcgcgagc
     4741 tcggcgatcc agccgctaaa accggcgacg ccgcagccag ccttgccgtt cgatgcgctg
     4801 ctgcagccgg cggagcgctt gccgccctgc tatgccctgg aagagagcga atgtcatgct
     4861 gcgtcaaata gcaagacttc cgtggacgct gcggcacaga ccgaggccag tgacgcaggc
     4921 gaatcgccgc cggcagacaa ccagaacgta gccgaggctg aac
//
LOCUS       DASUGB010001219         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_415043, whole genome shotgun sequence.
ACCESSION   DASUGB010001219 DASUGB010000000
VERSION     DASUGB010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415043"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..721
                     /locus_tag="VFV87_23085"
     CDS             107..721
                     /locus_tag="VFV87_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5106732.1"
                     /translation="MASSHLKHAAPNHPILPVFAERWSPYAYDPRPVERAKLRACLEA
                     GRWAASSYNEQPWTFILAERTNEAEFKRMLACLVEGNQTWAKNAGVLMLTVVARNFVK
                     NGKPNKAAEHDMGMAAGNLVLQATALGLQGHQMIGIEPAKVRAEYQVPEGFDPHTALA
                     LGYPANVKPDTADPLEKRDLAPRTRKLLSEIVIAGTWGQSAKLE"
     gene            808..1803
                     /locus_tag="VFV87_23090"
     CDS             808..1803
                     /locus_tag="VFV87_23090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5106733.1"
                     /translation="MSVSIVIPICNELENIPRLYEQLAAVLPGMNQPWEIVFVDDGST
                     DGSVDALKELAASDERVKVVRFRRNYGQTAAMHAGIQHATGDVVVTLDGDLQNDPLDI
                     PMMVAKLDEGYDLVHGWRKQRHDTFITRKLPSLAANWLISKVTGFPIHDLGCTLKAIR
                     REIAQELELYGEMHRFIPILAHQRGARCVEVVTRHHPRRFGQTKYGLSRTVRVLLDLF
                     TVKYMLDYFASPMKLFGRFGLACCLLAIFAAAGAAGMKLGAGTDITGNPLTLLAVLGA
                     IAAIQFFSLGLLGEVNARIYYANQTKQNYAIRELVNCECGGLGGEAAEPSRRRAA"
     gene            1823..2344
                     /locus_tag="VFV87_23095"
     CDS             1823..2344
                     /locus_tag="VFV87_23095"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5106734.1"
                     /translation="MDAQPQLLLQTSKFRVVREIQTTPGGKTKTREIVRHPGACVIVP
                     LLDDGRVCLIRNYRIAVGQTLIELPAGTLEPPEPPHVTAERELIEETGYRAAKIGLLH
                     AFYLSPGILDEKMHLYLATGLTSGETAREEGEEIENWLVPWDEAVGMIFGGQIQDAKT
                     IVGLLWVDRLQQP"
     gene            complement(2432..3361)
                     /locus_tag="VFV87_23100"
     CDS             complement(2432..3361)
                     /locus_tag="VFV87_23100"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5106735.1"
                     /translation="MLSRVCVAAILVVFAGVLHAEERGIRLGEATTSTWRIGVTVRAP
                     GAVTGILATTPVPMDWPEQQVKIVSEEKTDNVAKLSYRTLDDGVKQMLVSIPRLNAGE
                     EASAIVTFEVTRREILAPDDTSVYQTPAKSSRELSKFLLPSPYIESTDAKIRMLATDI
                     TAGKEPSWERAAAAFDWVRANVKYEFAEQIKPAVQALQDGQGDCEELSSLVIALCRAS
                     KIPARAVWVPGHCYPEFYLVDDKGEGHWFPCQAAGADRQFGSMIEDRPILQKGDNFRV
                     PEERGPQRYVKQFLSAKNAAANPEVKFVLEKVE"
     gene            complement(3455..4414)
                     /locus_tag="VFV87_23105"
     CDS             complement(3455..4414)
                     /locus_tag="VFV87_23105"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5106736.1"
                     /translation="MIRRLACVFAVCSVLPATTWAQFDTKPADAAAGPRLGEAKPQNW
                     EFGVSIRAVGGPSAGLFGTFPVPADWPEQQVKVVNEEITPQVRRTSYRTTDGLKQCLF
                     DVPRLAPGETATCFLTLEIAKSPQQPPVDSSGLVVPKDPPREVRKYLGTSPHIESTNS
                     RIRNLARELTAAQSSAWEQVSAVQAGVRERIAFEADNKDDFKGAADALRDGKADKEDM
                     TAVFVALCRAAKIPARMVWVLDYCYAEFYLEDAAGKGAWYPCVVHEQANLGEVKDTRP
                     ILQKGDNFRVPESKETQWFVAEYLTGKGGGGQPSVEFRRRSAD"
     gene            complement(4565..>4851)
                     /locus_tag="VFV87_23110"
     CDS             complement(4565..>4851)
                     /locus_tag="VFV87_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106737.1"
                     /translation="HLHLTRHGLELPDAILSRWRRKMKKLMLLVMVAVLASNLAGCSS
                     CRRGWFSGWFNRGDQCDHFVPDDCHPGVPQATMMVPGPVMPGPIEIAPAG"
BASE COUNT          910 a         1568 c         1472 g          901 t
ORIGIN      
        1 gcccggcttc caaatgccga ttgcgcgatt gtttaagaat ctgggtcgtt cgtcaaagaa
       61 acgcaaataa cccgccaatt gcagcgcctg caatgagagg tcgttcatgg catcatccca
      121 tctcaaacac gccgccccga accatcccat cctccctgtc ttcgccgagc gctggagccc
      181 gtatgcctac gacccgcggc cggtggagcg cgcgaagctg cgcgcgtgcc tggaggcggg
      241 gcggtgggcg gcgtcgagtt acaacgagca gccgtggacg ttcatcctgg ccgagcggac
      301 gaacgaagcg gaattcaagc ggatgctggc ctgcctcgtc gaaggaaacc agacctgggc
      361 gaagaacgcg ggcgtgctga tgctgacggt cgtcgcccgc aacttcgtca agaacggcaa
      421 gccgaacaag gcggcggaac acgacatggg aatggccgcc ggcaatctgg tgctgcaggc
      481 gacggcactg ggtctgcagg ggcaccagat gatcggcatc gagccggcga aggtacgggc
      541 cgagtaccaa gtgccggagg gattcgatcc gcacacggcg ctggccctcg gctatccagc
      601 caacgtgaag ccggacaccg cggatccgct cgaaaaacgc gacctggccc cgcggacgcg
      661 caagttgctg tcggagattg tcatcgccgg aacctggggc caatcagcga agttggaata
      721 acgctagcag gttcgactgg cataggcggc ggtcgtcagg ttttctacac tccgcagccc
      781 gccaatctcc ctcctgcgag ccgacccatg tccgtctcga tcgtcattcc gatctgcaac
      841 gagctcgaaa acattccacg gctgtacgag cagctcgcag ccgtgctgcc cggcatgaat
      901 cagccgtggg agattgtgtt cgtcgatgac ggctcgacgg acggcagcgt ggacgccctg
      961 aaggagttgg ctgcgagtga cgagcgcgtg aaagtcgtcc gcttccgccg taactatggg
     1021 caaacggcgg cgatgcacgc cggcatccag cacgcgaccg gcgacgtcgt cgttacgctc
     1081 gacggcgacc tgcagaacga tccgctcgat attccgatga tggtcgccaa actcgacgaa
     1141 ggctatgacc tggttcacgg ctggcgcaag cagcgtcacg acaccttcat cacccgcaaa
     1201 ctgccctcgc tggcggccaa ctggctgatt tccaaggtga ccggatttcc gatccacgat
     1261 ctgggctgca cgctcaaggc gattcgccgc gagatcgccc aggagctgga gctgtacggc
     1321 gagatgcacc gcttcattcc catcctggcc catcagcgcg gcgcgcgatg cgtcgaagtc
     1381 gtcacgcggc atcacccccg gcggttcggg cagacgaagt acggcctctc gcgaaccgtc
     1441 cgagtgctgc tcgatttgtt taccgtgaaa tacatgctcg attattttgc cagcccgatg
     1501 aagctctttg gccgcttcgg tttggcatgc tgcttgctcg caattttcgc tgccgcgggc
     1561 gcagcgggga tgaagctcgg cgcgggaacc gacatcaccg gaaatcccct tacactgctg
     1621 gcagttctgg gagcgattgc cgcgattcaa tttttcagcc tcggattgct cggggaagtc
     1681 aatgcccgca tttattacgc caatcagacg aaacaaaact atgcgattcg ggaactcgta
     1741 aattgcgaat gcggcgggct gggcggcgaa gcggccgagc caagccgccg ccgtgccgct
     1801 tgagagccgt aaggacaaga tcgtggacgc ccagccgcaa ctactgctgc aaacgagcaa
     1861 gtttcgtgtc gtccgcgaga tccaaacgac gccaggcggc aaaaccaaga cgcgcgagat
     1921 tgtccgccat cccggggcct gcgtcattgt gccgctcttg gacgacggcc gcgtctgcct
     1981 gatccgcaat taccgcatcg ccgtcggcca gacgctgatc gagcttcccg ccggcacgct
     2041 ggagccgcct gagccgccgc acgtcactgc cgagcgcgag ctgatcgaag aaaccggcta
     2101 tcgcgccgcc aagatcgggc tcctgcacgc cttctatctc tcgcccggca ttctcgacga
     2161 gaaaatgcac ctctatctcg cgaccggtct cacctccggc gagacggccc gcgaggaagg
     2221 cgaagagatt gaaaactggc tcgtcccctg ggacgaagcg gtcggcatga tctttggcgg
     2281 acagattcag gacgcgaaaa cgatcgtggg cctcttgtgg gtggatcgac tccagcagcc
     2341 gtagggtggg acaaggccgt cgcggccgcc ggcccaccgc cacttgccgg acattggtgg
     2401 gcctgcgctc gctttgcccg ctggtcccac cctactctac tttctccagc acgaacttca
     2461 cctcgggatt cgcggcggcg ttctttgcgc tgaggaactg cttgacgtaa cgctgcgggc
     2521 cgcgttcttc cggcacgcgg aagttgtccc ccttctgcag gatcggccgg tcctcgatca
     2581 tgctgccgaa ttgccggtcg gccccggctg cctggcaagg aaaccaatgc ccttcgcctt
     2641 tgtcatcgac aagatagaac tccggatagc agtggccagg aacccacacg gcccgagccg
     2701 ggattttgct cgcccggcac agcgcgatga ccagcgacga gagctcttcg caatccccct
     2761 ggccgtcttg cagggcctgc acggccggct tgatctgctc ggcgaactcg tacttcacat
     2821 tcgcccgcac ccagtcgaac gcagccgcgg cccgctccca gctcggctct ttgcccgcgg
     2881 tgatgtcggt ggcgagcatc cggatcttgg catccgtact ctcgatgtac gggctaggga
     2941 gcaggaactt gctcagctcg cggctgctct tggccggtgt ttgatagacg ctcgtatcgt
     3001 ccggcgcgag aatctctcgg cgcgtcactt cgaacgtcac gatcgccgac gcttcctcgc
     3061 cggcattcaa acgtggaatg ctgacgagca tctgcttcac accatcatcg agcgtgcgat
     3121 agctgagctt ggcgacgttg tcggtcttct cttcgctgac gattttgact tgctgctcgg
     3181 gccagtccat cggcacgggc gtcgtggcga ggatacccgt cacggcgccg ggcgcgcgga
     3241 cggtgacgcc gatccgccac gtcgaggttg tggcttcgcc caggcggatg ccgcgctcct
     3301 ccgcatgcag cacgcccgca aaaacgacga gaattgcagc aacgcacacg cgactcagca
     3361 tacgatgacc tctaatccat gttgtccggc agcgagacgg caatcaagca gattgcactg
     3421 cgccggggta tttcgcgcta ctcgtggatc ctgcctaatc cgccgaacgc cggcggaatt
     3481 cgaccgaagg ctggccgccg ccgcctttcc ccgtcaaata ctcggccacg aaccattgcg
     3541 tctctttgct ttcgggtacg cggaagttgt cgcccttctg caggatgggc cgcgtgtctt
     3601 tcacttcacc caggttcgcc tgctcgtgga cgacgcacgg gtaccaggcc cccttgcccg
     3661 ctgcgtcttc gagataaaac tcggcgtagc agtagtcgag cacccacacc atccgcgcag
     3721 gaatcttcgc cgcccggcag agggcgacga atacggctgt catatcttcc ttgtccgcct
     3781 tgccgtcgcg aagcgcgtcg gcggcgcctt tgaaatcgtc cttgttgtcc gcctcgaaag
     3841 caattcgctc ccgcactccc gcttggacgg cggagacctg ttcccacgcg ctgctctgcg
     3901 ctgccgtcag ctcccgggcc aggttgcgga ttctgctgtt ggtgctttcg atatggggac
     3961 ttgtgccgag gtatttgcgc acttcccgcg gcgggtcttt cggaaccaca aggccgcttg
     4021 aatcgactgg cggctgctga gggctcttgg cgatctctag cgtgaggaag catgtggccg
     4081 tttcgcccgg cgcaagacgc gggacatcga acaggcactg cttgaggcca tcggtcgtgc
     4141 gatagctcgt ccgccgcact tgcggcgtga tttcctcatt gaccaccttc acctgttgct
     4201 cgggccaatc ggccggcacg ggaaacgtgc cgaagagccc ggcagaaggt ccgccgaccg
     4261 cgcgaatcga gacgccgaat tcccaattct gcggcttcgc ctcacccagg cgtggacccg
     4321 cggcagcatc cgcaggcttt gtatcaaact gggcccaagt cgtagcaggc agcaccgagc
     4381 aaacggcgaa cacgcaggcg agccgacgga tcatgctttc cccctacgcc ggcagggatg
     4441 gcgctgaagt agaggcgcgc cggcgtacag ggaaacatat cttgcagggc agacttggcc
     4501 cgccagagcc gccggaccat gctcgcagtc cgcagcggca atcggttcaa ttgatacgca
     4561 aggcttagcc ggccggtgcg atttcaattg gcccgggcat gacaggaccg ggaaccatca
     4621 tcgtggcttg cggaacgccg gggtggcagt cgtcgggaac gaagtgatcg cattggtccc
     4681 cgcgattgaa ccagccgctg aaccaccctc ttcggcagga actgcaaccc gccagattcg
     4741 atgccaaaac tgcaaccatg accagcagca tgagcttctt catcttcctc ctccagcgcg
     4801 agagaattgc gtcaggcaac tctagcccgt ggcgagtgag gtgcaaatgc a
//
LOCUS       DASUGB010001220         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_135581, whole genome shotgun sequence.
ACCESSION   DASUGB010001220 DASUGB010000000
VERSION     DASUGB010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135581"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..383
                     /gene="fliQ"
                     /locus_tag="VFV87_23115"
     CDS             108..383
                     /gene="fliQ"
                     /locus_tag="VFV87_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX5106738.1"
                     /translation="MDAQDAVTLVQQALLTALIVAAPILLVGMAVGLLIGLAQALTQV
                     QDQTVSFVPKIIAMVAVLVFCLPWLSTKLVEYSEYLIVNIPRTIAGG"
     gene            465..1277
                     /locus_tag="VFV87_23120"
     CDS             465..1277
                     /locus_tag="VFV87_23120"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX5106739.1"
                     /translation="MSALLHLYLDQFLIFVLVLTRVGCLLMSLPVLGTASVPMHVRAL
                     LAVAISLVITPLYWGLSIPIPENLLDLSLLLVREAELGLALGLAVMILLSGMQLAGQV
                     VSQISGLSLADVVNPSFDTTVPIFSQILEMLAVAIFFLVGGHRQVIDALLGSFTWMPP
                     GQGRLPDDMVAALSAVAAESFEVGIRASAPVMVALLLSTLIVALVSRTLPQLNAVAVG
                     LNFNSLIVLAVLAFGIGSAGWVFQEELSSVMEHVGQAFVPAEDQSTRTIGYR"
     gene            1320..2405
                     /locus_tag="VFV87_23125"
     CDS             1320..2405
                     /locus_tag="VFV87_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX5106740.1"
                     /translation="MASEEFGDKTHEATPHRRQKAREEGQVVRSQDLASAGLLVAGLL
                     ILWYFGQGVMEFFGRLASDQLGGEAWLAIDTQTAAHESAAIAWRMAIAVVPVLALVTL
                     AAVLAQMGQFGFLYLPQKLVLDWQRLNPLQNAQRIFSLTSVVHLGFGLLKVGLIFAVA
                     GWSLWGERGRLIGLTEHSSPEIAAYLLNVLFWTSLKIGGALAALALFDYGYQYWKHEQ
                     DLRMTTQELKEEIKTQQGDPQIAARRKQIQRQMVLHRLSSTIPKADAIVTNPTELAIA
                     LQYDPDKMAAPVVLAKGAGVLAQRIRRLGLENNVPIVERKELARALYANVEIGHPVPA
                     ELYAAVAEVIRYVYQLKGKKLPVARAA"
     gene            complement(2420..3313)
                     /locus_tag="VFV87_23130"
     CDS             complement(2420..3313)
                     /locus_tag="VFV87_23130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5106741.1"
                     /translation="MNDSDSPPLPALVLPLASAMMYVIGALLLRRAADYGVGFWRTTF
                     VANLICVVVFAPLWLLGGTFHLELLWQPAVVALLFILGSVLNFISLDRGDVSIATPVL
                     GIKIILVALFSTLFGGQFVPLKVWLAAALSTTAIALLNRTRATRHHHVTSTILAAGSS
                     AASFALFDVLVQKWSPAWGLGRFPPIMLGFVAIYVLGLVPRFPGPLKAIPGPAWLWLL
                     SGSLVMAVQSLIFVGTVAAFGHATTANVIYSSRGLWSVVVVWIIGHWFHNTEQQLGSG
                     IFRWRLVGSALMLAAIALVLW"
     gene            complement(3310..3903)
                     /locus_tag="VFV87_23135"
     CDS             complement(3310..3903)
                     /locus_tag="VFV87_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106742.1"
                     /translation="MTDEKMLGQIPHWIASGGPYLLLPADSVNHWSAVAQFEHPRESL
                     PASPLRNYDEACRIAAQHDIGLIELDDFSGIVLGGGDIPMATWLSRPNRIGADIVVAL
                     EWGFDYKDVNLAAEVASLDDSAFKSIGVSFRLLNADLVLFAAAESPPPFCFGSLHCPI
                     VPGTYRIASMQCEFGDYLVVRVHRLIATSVARGSGTE"
     gene            complement(3900..>4796)
                     /locus_tag="VFV87_23140"
     CDS             complement(3900..>4796)
                     /locus_tag="VFV87_23140"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5106743.1"
                     /translation="TEIKEPPVFIIGHWRSGTTFLHELMHLDERFGSPTTYECFAPHH
                     CILTEWWVTLFFGWLMPRQRPMDNMVMGWSRPQEDEFALLTLGAPTPYLRMAFPNDGP
                     VCLEFLDMQGCSEADLKEFEQALMHFAQIITYKTEKRLLFKSPPHTGRIEVLSKLFPG
                     AKFIHITRHPDSLFPSTVRLWKSLDDVQALQWPKNEGLEEYVFECLTRMYRGFEQQRP
                     KIDPANIIDLRYEDLVADPVGEVQRIYQRLNLGDFEPVREKLTTFVGEQKDYQTNKHS
                     LDPELRERIRERWAGYCERYGY"
BASE COUNT          924 a         1491 c         1498 g          883 t
ORIGIN      
        1 ggcatgttgc tcgaaagctt cggcggaccg ccctgaccaa agccgcgacc agtggtcgcg
       61 gttcctacgc tttataccgc gaccactggt cgcggctttg agcttccatg gacgcccaag
      121 acgccgtcac gctcgtgcag caggcgctgc tgaccgcact gatcgtggcg gcgccgatcc
      181 tgctcgtcgg aatggcggtc ggactgttga tcggcctggc tcaggcgctc acgcaagtgc
      241 aggaccagac cgtgtcgttc gtgcccaaga tcatcgccat ggttgccgtc ctcgtcttct
      301 gcctcccctg gctcagtacc aagctggtcg agtactccga gtacctgatt gtgaacattc
      361 caaggacgat tgccggaggg tgagtgagtg gtgagtaggt gagtgggtga gtcgtgactc
      421 ctgactcctg acccctgact cctgacccct ggcccctggc cgccatgtcc gccctgctcc
      481 acctctacct cgaccagttc ctgatcttcg tgctggtcct gacgcgcgtc ggctgcctgc
      541 tgatgtcgtt gccggtgctg gggacagcga gcgttcccat gcatgtacgg gcgcttttgg
      601 cggtggcaat ctcgctggtc atcacgccac tgtactgggg actgtcgatc ccaatacctg
      661 aaaatctgct cgacctttca ctgctcctgg tccgtgaagc cgagctggga ctggcgctgg
      721 ggctggcagt gatgattctc ctctccggca tgcagctggc cggacaagtc gtgagccaga
      781 tcagtgggct ttcgctggcc gacgtcgtaa atccctcgtt cgatacgacc gtgccgatct
      841 tttcgcagat cctcgaaatg ctcgccgtcg caatcttttt cctcgtgggc ggacaccggc
      901 aggtgatcga cgccttgctc ggctcgttca cctggatgcc gcccgggcaa ggccggctgc
      961 cagacgatat ggttgcggct ctttctgccg tggccgcgga aagctttgaa gtcggcatcc
     1021 gggcctcggc ccccgtgatg gtcgccttgc tgctttcgac gctgatcgtg gccctcgtca
     1081 gccgtacgtt gccgcagctc aacgcggtgg cggtcgggct gaatttcaat tcgctgatcg
     1141 tcctggccgt gctcgcgttc gggatcggct cagccggctg ggtgtttcag gaggaattga
     1201 gcagtgtgat ggagcatgtg gggcaggcat tcgtgcctgc cgaggaccaa tcgacacgta
     1261 ccatcggata tcgatagcga tctattgcgg caggcaagaa tgcctgcccc accagagcga
     1321 tggcctccga agaatttggc gacaagacgc acgaagcgac gccgcatcgc cgccagaagg
     1381 cgcgcgagga aggccaggtc gtgcgcagcc aggatttggc ttccgcggga ctgctcgtcg
     1441 ccggtctctt gattctgtgg tacttcggcc agggagtcat ggaattcttc ggccgcctgg
     1501 cgagcgatca gctcggcggc gaggcctggc tggccatcga cacgcaaacc gcggcgcacg
     1561 aatcggccgc aatcgcgtgg cggatggcaa tcgccgtcgt gccggttctg gcgctggtca
     1621 cgctggcggc tgtgctcgct caaatgggac agtttggctt cctctacctg ccgcaaaaac
     1681 tcgtcctcga ttggcagcgt ctcaatcctt tgcagaatgc ccagcggatt ttctcgctga
     1741 ccagtgtcgt ccatttggga ttcggattgc tcaaggtggg gttgatcttt gccgtggcag
     1801 gctggagcct gtggggcgag cgcggccggc tgatcggcct aaccgaacat tcgtcgcccg
     1861 aaatcgccgc ctatctgctg aacgtgctgt tttggaccag cctcaagatc ggcggagcgc
     1921 tggccgcgct ggccctgttc gactacggct accagtattg gaagcacgag caggacctgc
     1981 ggatgacgac gcaggagctg aaggaagaaa tcaaaaccca gcagggcgat ccgcaaatcg
     2041 ctgcccgccg caagcaaatc cagcggcaga tggttctgca tcgcctgagc agcacgattc
     2101 ccaaggccga cgcgatcgtc accaacccga ccgagctggc gatcgcgctg cagtacgatc
     2161 ccgacaagat ggccgccccg gtcgtcctgg ccaaaggcgc cggcgtgctc gcgcagcgca
     2221 tccgccggct gggcctggaa aacaacgtgc cgatcgtcga gcgcaaagaa ctggcccgcg
     2281 ccctctacgc caacgtggaa atcggccacc ccgtccccgc cgagctatac gccgccgtcg
     2341 ccgaagtcat ccgctacgtc tatcagctca agggcaagaa gctgccggtc gcgcgggcgg
     2401 cataggtcca ttggtcgccc taccacagca ccagcgcaat cgccgccaac atcagggccg
     2461 agccgaccag ccgccagcgg aaaatgccgc tgccgagctg ctgctcggtg ttgtggaacc
     2521 agtggccgat gatccagacg acgaccacgc tccacaggcc gcgggagctg tagatcacgt
     2581 tggcggtcgt ggcgtggccg aaggcggcga ccgtgccgac gaaaatcagc gattgcacgg
     2641 ccatcaccag gctgccgctg aggagccaca gccaggcggg gccgggaatt gccttgagcg
     2701 gcccgggaaa tcgcgggacc agccccagga cgtaaatcgc tacaaagccc agcatgatcg
     2761 gcggaaagcg ccccaggccc caggccggcg accacttctg caccagcacg tcgaacaggg
     2821 cgaacgaggc cgccgacgaa ccggcagcga ggatcgtgct cgtcacgtga tggtgacgcg
     2881 tcgcccgcgt gcggttgagc agggcgatgg ccgtcgtgct caaggccgcg gcgagccaga
     2941 ccttgagcgg cacgaactga ccgccgaaca gcgtcgaaaa cagcgcgacc aggatgatct
     3001 tgatccccag caccggcgta gcgatcgaaa catcgccccg gtcgagcgaa atgaagttca
     3061 gcaccgagcc gagaatgaac aagagcgcca ccactgccgg ctgccagagc agttccaggt
     3121 ggaacgttcc gcccaggagc cacagcggcg caaacaccac gacgcagatc aggttcgcga
     3181 cgaacgtcgt ccgccagaag ccgacgccgt aatcggccgc ccgccgcaga agcagcgcgc
     3241 cgatgacgta catcatcgca ctggccagcg gcagtactag cgcgggcagt ggcggcgaat
     3301 ccgagtcatt cattcggtgc cgctaccccg cgcgacgctg gtcgcaatca agcgatgtac
     3361 ccggaccacc aagtagtcgc caaactcgca ctgcatggat gcaattctgt aagtacccgg
     3421 gacgatgggg caatgtaaac ttccaaagca gaacgggggc ggcgactcgg cagcagcgaa
     3481 tagaaccaag tcggcgttga gcagcctgaa tgaaactccg atcgatttga aagctgagtc
     3541 atcaagggat gccacttccg ccgccaaatt gacatcctta tagtcaaagc cccattccaa
     3601 ggcgactacg atatctgcgc cgattcggtt gggtcgcgaa agccaagtcg ccatagggat
     3661 gtcgccgcca cccagcacga tgcctgagaa atcatccagc tctatcaagc cgatgtcatg
     3721 ttgcgcggca attcggcagg cctcgtcgta attccgcagg ggcgaagcgg gaagcgattc
     3781 acgaggatgt tcgaattgcg cgacggcact ccagtgattt acagagtctg cgggcaaaag
     3841 tagatagggt ccacctgacg caatccaatg tggaatctgt ccaagcatct tttcgtccgt
     3901 caatagccgt accgctcgca atagccggcc caccgctcgc ggattctctc acgcagctca
     3961 gggtcgagcg agtgcttgtt ggtctgatag tccttctgct cgccgacgaa agtggtgagc
     4021 ttctcgcgca ccggctcgaa gtcgccgagg ttcagacgct ggtagatgcg ctgcacctcg
     4081 ccgacgggat cggcgacgag gtcttcgtag cgcaggtcga tgatgttggc cggatcgatc
     4141 ttcggtcgct gctgctcaaa gccgcggtac atccgcgtga ggcactcgaa gacatattcc
     4201 tccaggccct cgtttttcgg ccactgcagg gcctgcacgt cgtcgagcga tttccagagc
     4261 cgcaccgtgg aaggaaagag cgaatcggga tggcgcgtga tgtgaatgaa cttcgccccg
     4321 ggaaagagct tggaaagcac ttcgatgcgc ccggtgtgcg gcggcgattt gaagagcagc
     4381 cgcttctcgg tcttgtacgt gatgatctgg gcaaagtgca tcagggcctg ttcgaactct
     4441 tttaagtcgg cctcggagca gccctgcatg tcgaggaatt ccaagcagac cggcccgtcg
     4501 ttcgggaaag ccatgcgcag gtagggcgtc ggagcgccga gcgtgagcag ggcgaattcg
     4561 tcctcctgcg gccgcgacca gcccatcacc atgttgtcca tcggccgctg cctgggcatc
     4621 agccagccga agaacagcgt gacccaccat tcggtcagga tgcagtggtg cggggcgaag
     4681 cactcgtaag tcgtgggcga gccgaatcgc tcgtcgaggt gcatcagctc gtgcaaaaac
     4741 gtcgtgccgc tccgccagtg accgatgatg aagaccggcg gctccttgat ttcggt
//
LOCUS       DASUGB010001221         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1525439, whole genome shotgun sequence.
ACCESSION   DASUGB010001221 DASUGB010000000
VERSION     DASUGB010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525439"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..746
                     /locus_tag="VFV87_23145"
     CDS             111..746
                     /locus_tag="VFV87_23145"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX5106744.1"
                     /translation="MTKWEFKGRELVNCTCEYGCNCQFNALPDKGHCHAVAGIQIDEG
                     HHGDTRLDRLRIGAIFKWPGPIHEGNGEALAFVDERADAAQRDALLRIMTGQDTDPFA
                     TMFAVYASTVTKMHEPVFTPIDFEVDVEGRRGRFSVPGYVEMRGEPIRNKVTGEESRG
                     QIVLPNGFEYTVAEIGSASSHTTGGPMQVEFGDSYGQFANLHLSSHGVVRN"
     gene            911..1504
                     /locus_tag="VFV87_23150"
     CDS             911..1504
                     /locus_tag="VFV87_23150"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX5106745.1"
                     /translation="MIVATMWWTMMIAMMVPSAAPTVLLYARVYRVRIDDGPSPPVGA
                     FLSGYLLCWLVFSLAAAAIQLWLQDAAIASPITMRLHTAGAAGALLILAGLYQLSPLK
                     NACLGKCRSPAQFLAHHYRPGPAGAFRMGAIHGSFCVGCCWLLMALLFVGGVMSIAWI
                     AALAITVAAEKLLGAGPSFAQVVGAALIVGGASLILS"
     gene            complement(1536..2369)
                     /locus_tag="VFV87_23155"
     CDS             complement(1536..2369)
                     /locus_tag="VFV87_23155"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5106746.1"
                     /translation="MRKAVIALLLIAGVTACDRKQESGEVAVSATPAQLTFDGADYKD
                     EASKIAHGRRLAVVLDCTGCHGADMQGKNVTADDPSYGQMNAPNVTLLLAKYSDADFD
                     RLIRHGVPKDAREFWFMPVESFQFLSDADLSAITAYLRSFKPAGTRLPPITKGKGFHE
                     DVARGFLDAQSQVRRYRETQPVDIGPQHSKGRYLVKVICTACHNGQLEGYRDFTPDLD
                     IAGAYSDAELTRLLSTGEGKVKKNLGMMSEMGRDHFSSLTPNERQTIVSYLKARAERP
                     Q"
BASE COUNT          453 a          768 c          735 g          508 t
ORIGIN      
        1 gtctccaacg gccgctcgtt gtagttaccc gccgaccgga gcaatcacgc gatttccatc
       61 gactccagat gctctggtaa aggaactgca agccgatcgc ggaggcgccg atgaccaaat
      121 gggaattcaa aggtcgcgag ctggtgaatt gcacctgtga atatggctgc aactgccagt
      181 tcaatgcatt gccggacaaa ggacattgcc acgccgtggc aggcatccag atcgacgagg
      241 gacatcatgg cgacacgcgg ctcgataggc tgcgcatcgg cgcgatcttc aaatggcctg
      301 gaccgattca cgaaggaaat ggcgaggccc tggccttcgt cgacgaacgg gccgatgcag
      361 cccagcgcga cgcgctcctg cggatcatga ccggccagga caccgatcct ttcgcgacca
      421 tgttcgcggt ctacgcttcg accgtgacga agatgcacga accggtcttt acgcccatcg
      481 atttcgaggt cgatgtcgag ggccggcggg gccgcttctc cgtccccggc tatgtcgaga
      541 tgcgtggcga gccgatccgt aacaaggtta cgggcgagga atcgcgcggg cagatcgtcc
      601 tgccgaacgg tttcgaatat accgtcgccg aaatcggaag cgcgtcgagc cacacgaccg
      661 gtggtccgat gcaagtcgaa ttcggtgata gctacggtca gtttgcgaac ttgcatctct
      721 caagtcacgg agtggtgcgc aactgacgaa agcaggtggc aaatccttgg cagaggcagc
      781 catccgccga aaccggtcga tcacgattgc cggccttata ctcctgacgc tgctggcctg
      841 ggcctatctc ttggcagggg cgggtatgcc ctcccaccca aaacatatgg ccatggaagg
      901 acctggcttc atgatcgtgg ccaccatgtg gtggacaatg atgatcgcta tgatggtacc
      961 gagcgctgcg cctacggttc tgctttacgc ccgggtctac cgcgtccgta tcgacgatgg
     1021 tccaagtccc cccgtcggag ctttcctttc cggatacctc ctatgctggt tggtcttttc
     1081 gctcgctgcc gccgcgattc agctttggtt gcaggacgcg gcaatcgcat ctcccatcac
     1141 gatgcggttg cacaccgccg gcgccgccgg agcactgctg atcttggccg gcctatacca
     1201 attgtcgcct ctcaagaacg cgtgcctggg caaatgccgc agtcccgcgc aattcctcgc
     1261 ccaccattat cgccccggac cggccggcgc cttccgcatg ggcgcgatcc atgggtcatt
     1321 ctgcgtcggg tgctgctggc tcctgatggc gctccttttc gtgggaggcg tcatgagcat
     1381 cgcatggatc gccgcccttg cgataacggt cgccgccgag aaattgctcg gtgccggccc
     1441 atccttcgcg caagtagtgg gcgcggcgct catcgtaggc ggcgcgtcac tgatcttgtc
     1501 ttagaggtct ccagcccgcg ttctggcatg atcagctatt gcggccgttc cgcccgcgcc
     1561 ttcaaatagc tgacgatcgt ctggcgttcg tttggggtca ggctcgagaa atgatcgcga
     1621 cccatctccg acatcatccc gagattcttc ttcaccttcc cctcgccggt cgagaggaga
     1681 cgcgtcaatt ctgcatcaga gtaggcgccg gcaatatcga gatcgggcgt gaaatcacgg
     1741 tacccttcga gctgaccgtt gtggcaagcc gtacagatta ccttgaccag atacctgccc
     1801 tttgaatgtt ggggtccgat gtcaacgggt tgcgtctcgc gatagcgccg gacctgcgat
     1861 tgagcgtcga ggaacccgcg ggcgacatcc tcgtggaagc ccttgccctt ggtgatcgga
     1921 ggaagccgcg ttcccgctgg cttgaaagac cgcagatagg cggtgatcgc cgacagatcg
     1981 gcgtcgctca ggaactggaa gctttccacc ggcatgaacc agaactcgcg cgcgtccttg
     2041 ggcacgccat ggcgaatgag gcggtcgaag tcagcatcgc tgtactttgc aaggagaagc
     2101 gtgacgttcg gcgcattcat ttgcccgtag gacggatcgt ccgcggtgac gttcttgccc
     2161 tgcatgtcgg cgccgtggca gccggtgcag tcgagcacca cggccagccg cctgccatgc
     2221 gcgatcttcg atgcctcgtc cttgtaatcc gcgccgtcga aggtgagctg tgccggggta
     2281 gccgagacag caacctctcc gctttcctgc tttcgatcgc aggctgtcac gcccgcaatc
     2341 aaaagcaacg cgatgaccgc tttgcgcatc atcacctccc ctgctcggcg acggcagtac
     2401 cgcagttcgt ccctctcgcc gagtcaggtg cttgacgctc aggaagtccc tggcctagct
     2461 taag
//
LOCUS       DASUGB010001222         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_832365, whole genome shotgun sequence.
ACCESSION   DASUGB010001222 DASUGB010000000
VERSION     DASUGB010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832365"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFV87_23160"
     CDS             complement(<1..774)
                     /locus_tag="VFV87_23160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106747.1"
                     /translation="MKPGAAALPARPALPSSGLEAGVEIGDFRIERRLGAGGMGIVYQ
                     ARQLSLNRPVALKVLGQALSRGTDVTRFQREAQAAARLRHPAIAHVYFIGQDRHLCYL
                     AMELIEGASLRKVLDRLGTAATSESSLDSVVRDDLSDDPEARVVRFDVATEDTSASPP
                     DALPAEINPYLSPQARELRTSAGYVRRACEVVRDAARALAHAHEQGVIHRDIKPDNLL
                     LDRHGRVHLIDFGVARFFEDQAVTYTGQLVGTPLYMSPEQ"
     gene            832..1890
                     /locus_tag="VFV87_23165"
     CDS             832..1890
                     /locus_tag="VFV87_23165"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5106748.1"
                     /translation="MRTNLPRRTCSPLGPYSAPVAIVALGIAVGCLSSRKANEQVVVY
                     AALDREFSGPVLDDFARQSGILVLPKYDIESTKSVGLASAIVAEASHPRCDVFWNNEI
                     LNTLRLADQGRLDVYQSPAAAGFPAMYRAKNGAWHGFAARGRVLVVNTNLVPPEDRPR
                     SIYDLADARWKGKVGIARPVAGTTATHVACLFAVLGPDKAKQLLRNMRVNDIRIYGGN
                     KQVAVAASTGEIAFGLTDTDDAVGEVERAAPVTIVYPDRGADELGTLFIPNTVSIVKG
                     CPNPAAARRLVDFLLSPAVEAQLARGASAQIPLNPEVEAKLRVETPRTVKAMPIDFAA
                     AAGKWDAAAEFIEEEFLK"
     gene            complement(2009..>2256)
                     /locus_tag="VFV87_23170"
     CDS             complement(2009..>2256)
                     /locus_tag="VFV87_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106749.1"
                     /translation="AYALAGDGLAAVRHAERCLSLSVQNARDESPFDRATALGCAARA
                     HTLAGDSAQGDRLRTLAKTAAAALDDDDRGVFEKLYG"
BASE COUNT          409 a          723 c          728 g          396 t
ORIGIN      
        1 ttgctcgggg ctcatgtaaa gcggcgtgcc gacgagctgg cccgtatagg tcaccgcctg
       61 atcttcaaaa aatcgggcca cgccgaaatc gatcaaatgc acgcggccat ggcgatccag
      121 cagcaggtta tcgggcttga tgtcgcgatg aatgacccct tgctcgtgcg cgtgggcgag
      181 cgctcgggcg gcgtcccgca ccacctcgca cgcgcggcgc acgtagcccg cggacgtacg
      241 cagttcgcgg gcctgcggcg agagataggg attgatctcg gccggcaaag cgtcgggcgg
      301 gctggcgctg gtgtcctcgg tcgccacgtc aaacctcacg actcgcgcct ccgggtcatc
      361 cgacaggtcg tcgcgcacga cagaatcgag gctcgactcg cttgtcgcgg ctgtccccag
      421 ccgatcgagc accttgcgga gcgaagctcc ctcgatcagc tccatcgcca ggtagcacaa
      481 gtgccggtct tgcccgatga aatagacatg ggcgatggcc ggatgccgca ggcgggccgc
      541 ggcctgggcc tcccgctgaa agcgggtaac gtcggtgccg cggctgagcg cctggcccag
      601 caccttcagg gcgactgggc gattgagcga aagttgacgg gcttgataaa cgatacccat
      661 gccccccgct cccagacgcc gctcaatgcg gaagtcgccg atttccacgc ccgcctccaa
      721 tccgctggag gggagcgctg gtcgagcggg cagtgcggcg gcgcctggtt tcataagccg
      781 tgtaacctcg ggagcaggca gtgagtacta tcgtacttga aggacgtggc catgcgaacc
      841 aacttgcccc ggcgcacctg ctcgcctctg ggaccatact cggctcccgt tgcaatagtt
      901 gcactaggaa tcgccgtcgg ttgcctatcc agccgcaagg ccaatgagca agtcgtggtc
      961 tatgccgcct tggaccggga attctctggc ccggtgctgg acgacttcgc tcgccagtcc
     1021 ggcattctcg tgctcccgaa atatgacatc gaaagcacca agagcgttgg gctggccagc
     1081 gccatcgtgg ctgaggccag ccatccgaga tgcgacgtgt tctggaacaa cgaaatcttg
     1141 aacacgttgc gactggcgga ccaggggcgg ctcgatgttt accagtcccc cgctgccgct
     1201 ggctttcccg ccatgtatcg cgcgaagaat ggcgcgtggc acggctttgc ggcccggggc
     1261 cgggtgctgg tagtgaacac gaaccttgtg cccccggagg atcggccccg atcgatctac
     1321 gatctggctg atgccaggtg gaaaggcaag gttggcattg cccgcccggt cgccggcacg
     1381 accgccacgc acgtcgcctg tctgtttgcc gtgctgggcc cggacaaggc caaacaacta
     1441 ctgcgcaaca tgcgagtcaa cgacattcgc atctacggcg ggaacaagca ggttgccgtg
     1501 gccgcctcga ccggcgagat cgcgttcggc ctgaccgaca cggacgacgc cgtcggcgaa
     1561 gtcgagcggg ccgcgccggt gacgatcgtc tatcccgatc ggggcgcgga cgagctgggg
     1621 acgctcttta tccccaatac cgtctcgatt gtcaaaggat gcccgaatcc ggctgcggcc
     1681 cgcaggctag tcgatttcct gctctccccc gccgtcgagg cgcagctagc taggggcgcc
     1741 agcgcccaga ttccactcaa tccggaggtc gaggcgaaac tgcgcgtcga aacaccccga
     1801 acggtgaagg cgatgcctat cgattttgcg gcagcggccg ggaaatggga cgccgctgct
     1861 gaattcatcg aggaggagtt cctgaagtag tcagcgcgag atcgccgtgg gcatcagtgg
     1921 gtcggcactt gttccggacg tttcatccag tgttagaacg tcgatcctga aagtgttcgc
     1981 ccctcgggaa cgaaactcag agtgagtcct acccgtagag tttttcaaat acgccgcgat
     2041 cgtcgtcgtc caaggcggct gcggcggttt tggccagcgt tctcaggcgg tccccctgcg
     2101 cggaatcgcc agccaacgta tgcgcccgag cagcgcagcc cagggccgta gctcgatcga
     2161 acggcgattc gtccctggcg ttctgcacgc ttagcgacag gcaccgctcg gcatgacgga
     2221 cagcggccag accgtcgcca gccagggcgt aggccg
//
LOCUS       DASUGB010001223         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1388410, whole genome shotgun sequence.
ACCESSION   DASUGB010001223 DASUGB010000000
VERSION     DASUGB010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388410"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2118
                     /locus_tag="VFV87_23175"
     CDS             <1..2118
                     /locus_tag="VFV87_23175"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5106750.1"
                     /translation="WCGVKCEKFFIGFDIGGYKISRKWGETEYGIGILPLGGYVKMLG
                     QDDDPRNAEAEAARTRAEQPGPSDQARAEAIASGSAAEGLVTGQTVERASQQALPPNA
                     VPAKASDGSTVLLDPRSYPARPIPARMAIISAGVIMNLIFGVILGALAYKLGINEMPA
                     IIGGTTPGSVPWTLGIPPGSQALQIGESGQPYEHLRWEDFRFTAILNTNSEVPILFRK
                     PNGEEEWFNVRPTKPPGSDVPLIGLAGPRDRQVNIFPEFAAHLNPAATPTLQDFDVVV
                     EAAGQEVTSGEDLQAIFARQPLGEMKIKVERPAPEDLKKRRWEWQKSPQTIDVVLTPR
                     PIRELGVAMKIGPVLAVRENSPAARAGILVDDQILEIDGQPLGDPLSLSQRLTPGDEP
                     QDVTITVNRKGAGGKPERKTFTLTPEAPLQSMSLLLQSVAGANPISIEPIGVAFQVTS
                     EVESVAAGGPAEKAGLQSGDIVTKVEWQPGVSDDLAGKLQSIVNPMVFETIDLQDKGT
                     WPTVASRLQAVVPQTKARLTWTRKGKEMSAVLEPRDAADFVDDARGIALYPLEVEHVA
                     SDWGDAFALGFRETKDRLFQTLLILQRLVTGRLSPTNLSGPLGIIAAAGAFASQGFPD
                     LLMFLVLLSANLAILNFLPIPALDGGHMLFLTAEAIRGRPVDEKLQVRLTVAGVVCLL
                     SLMVFATAMDIGRIAQWMQRWFG"
     gene            2128..2829
                     /locus_tag="VFV87_23180"
     CDS             2128..2829
                     /locus_tag="VFV87_23180"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5106751.1"
                     /translation="MQSGIRWILFDAVGTLIYPRPPVAEVYRDGGRQFGSLLDAEEIA
                     TRFRRALAAENDGHESLTRPPTSEFAELARWRRIVAAVLDDVPEGGQARLFQSLWRHF
                     AQPGHWRVFDDVQPALLQLADAGFRLGIASNYDGRLRTIVAAHAELAHCEQIFVSSQV
                     HFTKPDPRFFRAVGEQLEAEPAEILLVGDDWLADIEGARASGWQAIFLNREAELRDAT
                     TIASLSELPSRYGAR"
     gene            complement(2814..3617)
                     /locus_tag="VFV87_23185"
     CDS             complement(2814..3617)
                     /locus_tag="VFV87_23185"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5106752.1"
                     /translation="MPRKKRPTDCKHRLRKRLFRDNALLQAGLGFGARGLLQLWLGTM
                     RVWIEFEDPALDPRQGGLGAVFCIWHEHLLFLGHRFRDCGLSVMISKSADGELISRIV
                     SCLGYRTVRGSTGASGSRAVRELLHDARLGSLVLTPDGPRGPRRVFQAGAVYLASRLG
                     LPMAAAGCAYERPWRARTWDQLVLPRPFSRAVLVVGRGLKVPADADQDDLQIVRQQAE
                     GEMGVMNDRAEALLQRWVRTGETPRAARPAELPNVAQRGRLAMAAQRAP"
     gene            3813..>4172
                     /locus_tag="VFV87_23190"
     CDS             3813..>4172
                     /locus_tag="VFV87_23190"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HEX5106753.1"
                     /translation="MKLRSPWIVPLAALFAAIVIRLWLCTLRIRSVSVDGRQHPIDPA
                     TLKCIYAFWHEGMLALLSAKAKGRVLVSKSADGEFIAQVCGFLGIGVVRGSSSRGGHW
                     SVAGQRGGFEALSEMIRS"
BASE COUNT          721 a         1350 c         1363 g          738 t
ORIGIN      
        1 tggtgcggcg tgaagtgcga gaagttcttc atcggcttcg acatcggcgg ttacaagatc
       61 agccgcaagt ggggtgaaac cgagtacggc atcggcatcc tgccgctggg cggctacgtg
      121 aagatgctcg gccaggacga cgacccccgc aacgccgaag ccgaagccgc ccgaacacgc
      181 gcagaacaac ccggcccctc cgaccaggct cgggcagaag cgatcgcttc cggttccgca
      241 gccgagggtc tggtcaccgg ccaaaccgtg gagagggcca gtcaacaggc attgcctccc
      301 aacgcggttc cggcgaaggc gtcagatggc agcacggtcc tgctcgatcc gcggagctat
      361 cccgccaggc cgatccccgc ccggatggcg attatctcgg ccggcgtgat catgaacctg
      421 atcttcggcg ttattctcgg ggcgttggcc tacaagctgg ggatcaacga gatgccggcg
      481 attatcggcg gaacgacgcc cggcagcgtg ccgtggacgc tcggtattcc gcccggatcg
      541 caggccttgc aaatcggtga gtcgggccag ccctacgaac atctgcggtg ggaggatttt
      601 cgcttcacgg cgatcctcaa taccaacagc gaagtgccca tcctgttccg caagcccaac
      661 ggcgaagagg agtggttcaa cgtccggccg acgaagccgc cgggcagcga cgtgccgctg
      721 atcgggcttg cggggccgcg cgaccggcag gtcaatattt ttccggagtt cgccgcgcac
      781 ctgaatccgg cggccacgcc caccttgcag gacttcgacg tggtggttga ggcggcggga
      841 caggaagtga ccagcggcga ggacctgcaa gcgatctttg cccgccagcc gctcggagag
      901 atgaagatca aagttgagcg tccagcgccg gaggacctca aaaagcggcg gtgggagtgg
      961 cagaagtcgc ctcagacgat cgacgtcgtc ctcacgccgc ggcccattcg cgaattgggc
     1021 gtggcgatga aaatcgggcc ggttctggcg gtccgcgaga actcgccggc ggcgcgggcc
     1081 ggaatcctgg tggacgacca gattctggag atcgacggcc agccgctggg cgatccgcta
     1141 tcgctttcgc agcgcttgac gccgggcgac gagccgcaag acgtgaccat caccgtcaac
     1201 cgcaaaggtg cgggcggcaa gccggagcgg aagacgttca cgttgacgcc cgaggcgcca
     1261 ttgcagtcta tgtccttgct attgcagtcc gtcgcggggg ccaacccaat ctcgatcgag
     1321 ccgattggcg ttgccttcca ggtcacgagc gaggtcgagt ccgtcgccgc gggcggcccg
     1381 gctgaaaagg ccggactgca gagcggcgac atcgtgacca aggtcgagtg gcagccgggc
     1441 gtaagcgacg atctggccgg gaagttgcag agcatcgtca atccaatggt atttgagacg
     1501 atcgatctgc aggacaaagg tacgtggccc acggtcgcgt cgcggctgca ggcggtcgtt
     1561 ccgcagacga aggccaggct gacgtggacg cgcaagggga aggagatgag cgccgtctta
     1621 gagccacgcg atgcggctga tttcgttgac gacgcgcggg gaatcgccct ttatccgctg
     1681 gaggtcgagc acgtcgcctc cgactggggc gacgcgttcg cacttggctt ccgcgagacc
     1741 aaggatcgat tgtttcagac gttgttgatc ctgcagcggt tggtgacggg ccgcctctcg
     1801 cccaccaacc tttccggccc gctgggcatc atcgccgcag cgggggcgtt cgcttcgcag
     1861 ggctttcccg acctgctgat gttcctcgtg ctgctcagcg cgaacctggc gatcctgaat
     1921 ttcctgccga tcccggccct cgatggcggc cacatgctgt tcctcacggc cgaggccatc
     1981 cgcggcaggc cggtcgatga gaagctacag gttcgcctga cggtggctgg cgtcgtgtgt
     2041 ctgctatcgc tgatggtgtt tgccacggcg atggacattg gccgaattgc gcagtggatg
     2101 cagcggtggt ttggctaacg gtgacccatg cagtcaggca tccgctggat cctgttcgac
     2161 gccgtcggaa cgctgattta tccccgtccg ccggtcgctg aggtctatcg agatgggggc
     2221 cgccagttcg gctcgctact cgatgccgag gaaattgcga cacgatttcg ccgagctttg
     2281 gccgcggaga atgacggcca cgagagcctg acgcggccgc cgacgagcga attcgcggaa
     2341 ttggctcgct ggcggcggat tgtggccgcg gtcctggacg atgtgccgga gggcggtcag
     2401 gcgcgattgt ttcagtcgct gtggcggcat ttcgcccaac ccggccactg gcgcgtgttt
     2461 gacgacgtgc agccagccct tctacagctc gcggacgcag gattccgcct gggcattgcc
     2521 tcgaactacg acggccgttt gcggacgatc gtcgccgcgc atgcggagct ggcccactgc
     2581 gagcagatct ttgtctcctc gcaagttcac ttcaccaagc cggacccgcg gttctttcgc
     2641 gccgtgggcg agcagctcga agcggaaccg gcggagattc tgctggtcgg tgacgactgg
     2701 ctggccgata tcgaaggcgc gcgggccagc ggctggcagg cgattttcct gaaccgtgag
     2761 gcagaactgc gtgacgcgac gacgatcgcc tcgctgagcg aactgcccag ccgctacggc
     2821 gcccgctgag ccgccatcgc caatcgaccg cgctgggcca cgttcggcaa ttccgccggc
     2881 cgagctgctc ggggagtctc accggttcgc acccagcgct gcagcagggc ttccgcccgg
     2941 tcgttcatca cgcccatctc gccttccgcc tgctgccgca cgatttgcaa gtcgtcctgg
     3001 tcggcgtcgg ccggcacttt gagcccgcgg cccaccacca gcaccgcccg agaaaacggc
     3061 cgcggtaaga cgagctgatc ccaagtgcga gcccgccacg gccgctcgta cgcgcacccc
     3121 gccgccgcca ttggcagccc caatcgcgag gcgagataga ccgcgcccgc ttgaaaaacg
     3181 cgccgcggcc cgcgcggccc gtcgggcgtg agcacgaggc ttcccagtcg cgcgtcgtgc
     3241 agcagctctc ttaccgctcg gctgccgctc gctccggtcg agccccgcac cgtgcggtaa
     3301 cccaggcagc tcacgatgcg gctgatcagc tcgccgtccg cgcttttgct gatcatcacg
     3361 ctcaggccgc aatcgcgaaa gcggtgcccc aaaaagagca ggtgctcatg ccaaatgcaa
     3421 aagaccgctc ccaatccccc ctgccgcggg tctagcgccg ggtcttcgaa ctcgatccag
     3481 acccgcatcg tccccagcca aagctgcaag aggccgcggg cgccaaagcc caatccggcc
     3541 tgcaatagcg cattgtcgcg aaacaggcgc ttgcgaagac ggtgcttgca gtctgtgggt
     3601 cgtttcttcc tgggcattct tagcttccat gcacccggtc gacagcgatt gtagggcatg
     3661 tgaaccgaaa cttcctacgg cacctggcgt ctggctcgcg gcccccggcc ccgcgctctg
     3721 gcaattgacg tggacggcaa gcgcgggcga aagtagcctc cacgtccctc tcatctaaag
     3781 caacttccag tttttgccga tcttctgctg gcatgaaact ccgcagtccc tggatcgtcc
     3841 cactggcggc cctgtttgcc gcaatcgtga tcaggctgtg gctgtgtacg ctgcggatcc
     3901 gctcggtcag cgtcgatggt cgtcagcatc cgatcgaccc ggccacgctc aaatgcattt
     3961 acgccttctg gcacgaaggg atgctagccc tgctgtcagc gaaggcaaaa ggccgggtcc
     4021 tcgtgagcaa aagcgccgac ggcgaattca tcgctcaagt ttgcggcttt ttggggattg
     4081 gcgtcgtgcg cgggtccagc tcccgcggcg ggcattggtc ggtggccggg cagcgcggcg
     4141 gattcgaggc cctttccgaa atgatccgtt cg
//
LOCUS       DASUGB010001224         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_135753, whole genome shotgun sequence.
ACCESSION   DASUGB010001224 DASUGB010000000
VERSION     DASUGB010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135753"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2836)
                     /locus_tag="VFV87_23195"
     CDS             complement(<1..2836)
                     /locus_tag="VFV87_23195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5106754.1"
                     /translation="MTERELFLSALEIDDPASRQAHLQSSCGGNAELLSRVQSLLASH
                     ESQSQFLQTPALEQMGDDSAVGTAATLLVGAAEQRRDEQDDEIPLGYLQPSTRPGSLG
                     RLDHYEVLEVLGRGAFGIVLKAFDEKLQRVVAIKVLSPEMASTSPARKRFLREARASA
                     QIRHENVVSIYNVEEQPLPYLVMEYIPGETLQHRLDSQGPLDLEGVLRLGKQIADGLA
                     AAHAQQLIHRDIKPGNILLETSIKDHVKLTDFGLARTADDASVTQSGLIAGTPMYMAP
                     EQALGQQLDHRADLFSFGSVLYQMLSGRPPFRAPTTVAVLKRVAEDTPRPIREIIPEV
                     PDWMCQIVARLHAKNPDERYGSAKEVSELLDYCATELQQGRTPNIATAEPAPKPAPEP
                     VTPAPPKPSVAATKSRSRQPLLTAAAVALILLIGLGITEATGVTKLATTVIRLTTNSG
                     TLVIETDDPRLKVTVDGDNVRIHGSGLGELTLRPGQYTVAALKDGKPVKQELVSITRN
                     GKTVVRMTLEPDGEISQPVTSSGVTSAPRALLKNTLLGFPGGVLAVDYSPDGQLLASA
                     GDGEGESKVRVWNAESGELRYSVSVPARKFHTAVTFSPDSRYLLTGPIAASPNCPISI
                     WEASTGKPAGELMGHETALFELSFSPDGTTLISCGFDHVVRLWDFPSRKVLREIPTPG
                     NTWARSAVISKSGLIALGSDNVYLMDSDGKLLKTFEVHRPGRLVFSPDGKRLAGTTYA
                     EGLVTILDVETGNEIASWRAHNGTAIGVAFSRDGKVLVTAGGDGKVRFWDVNTQRELA
                     HVSHDGAYWVAFSPDGQTLATTGFDRVVKLWDVSAILAQGGKKVTDAEPFQWPAEAPP
                     PAIAPFNAEQAKAHQQAWAKCLGVPVEKEISLGRNQRGQDVALSLVLIPPGEFLMGTS
                     QDQIDTLREQAPATGLKVEDVGRINLEGPQH"
     gene            complement(2992..>3445)
                     /locus_tag="VFV87_23200"
     CDS             complement(2992..>3445)
                     /locus_tag="VFV87_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5106755.1"
                     /translation="VTRPGVRSQESGVRGEPAATGNLTPDSCLLTPQFSSRGHFFSAA
                     AEAMRRILVENARRKNRRKRGGDLAREELVEDAVAAPEIHEDLLALDAALDRLKEIDP
                     QAVQLVHLRYFVGLSIPQAAEQLGISPRSADRLWAFARAWLHREIAGQ"
BASE COUNT          567 a         1085 c         1133 g          660 t
ORIGIN      
        1 gatgctgagg gccttcgagg ttaatgcggc ccacgtcttc gactttgagt ccggtggccg
       61 gcgcctgctc gcgcaaggtg tcgatctggt cctggctggt ccccatcaga aactcacccg
      121 gcggaatcag gaccagactc agcgcgacgt cctgaccgcg ctggttccga cccagcgaga
      181 tttccttctc caccggcacg ccgaggcact tcgcccacgc ctgctgatgg gccttcgcct
      241 gctcggcatt gaacggcgca atcgccggcg gcggggcctc ggcgggccat tggaagggtt
      301 cggcatccgt caccttcttt ccgccttgcg caagaatcgc cgacacgtcc cacagtttga
      361 ccacccggtc gaaacccgtg gtcgccagcg tctggccgtc cggggagaag gcgacccagt
      421 aggctccgtc gtgtgacacg tgggccaatt cgcgctgggt gttcacgtcc cagaagcgga
      481 ccttgccatc gccgcccgca gtgacgagga ccttaccatc gcgggaaaag gcaactccga
      541 tggcagtgcc gttgtgggct cgccaggatg cgatttcgtt accggtctcg acgtccaaga
      601 tggtgacgag cccctccgca tacgtcgttc cggccaatcg ctttccgtcc ggcgagaaga
      661 caagacggcc gggtcggtgt acctcaaacg ttttgagtaa cttcccgtcg gaatccatga
      721 ggtaaacatt gtccgatccc agcgcgatca gcccggattt cgagatcact gccgatcgcg
      781 cccacgtgtt accgggtgtc ggaatctcgc gcaggacttt ccgtgaagga aagtcccaca
      841 gtcgaacaac gtggtcgaat ccgcacgaga tcaacgtcgt gccatctggc gagaatgaaa
      901 gttcgaacag cgcagtttca tgccccatga gttctccagc gggtttcccg gtgcttgctt
      961 cccagatgct gatcgggcaa ttggggcttg ctgcgattgg gccggtcagc agatagcggc
     1021 tatcgggaga gaaggtgacg gcggtgtgaa atttcctggc agggaccgac acggaatagc
     1081 gaagttctcc ggactccgcg ttccatacgc gcactttgct ttctccttca ccgtctcctg
     1141 cgcttgccag tagttgacca tccggagaat agtcaaccgc caaaactccg cctgggaagc
     1201 ccagcagcgt gttcttcagg agagctcgcg gggcggacgt gaccccggag gaggtaaccg
     1261 gctgggagat ctccccgtcg ggctccaggg tcatccgcac cacggtcttg ccgttacggg
     1321 tgatcgagac cagttcctgc ttgaccggct tgccgtcttt cagggcggcg accgtgtatt
     1381 gaccgggcct cagggtgagt tcgcccagac cggaaccgtg gatgcggaca ttgtctccgt
     1441 ccacagtgac ctttagccgc ggatcgtcgg tttcgatgac cagcgtgccg gagttcgtcg
     1501 tcagccgtat gacggtcgtt gccaacttgg tgacacccgt cgcctcggta attcccaggc
     1561 cgatcagcag aatgagggcg acggcggcgg cggtcaggag cggttggcga gagcggctct
     1621 tggtcgcggc gacgcttggc ttgggcggag cgggtgtgac tggctcgggc gcgggttttg
     1681 gcgcgggctc ggcggtcgcg atgttcggcg tccggccttg ctgcagctcg gtcgcgcaat
     1741 agtccagcag ttcgctcacc tccttcgccg agccataccg ctcgtccgga ttcttggcgt
     1801 ggagcctggc aacaatctgg cacatccagt caggaacttc cgggatgatt tcccgaatcg
     1861 gccgcggcgt gtcttcggcg acgcgcttga ggaccgcgac ggtcgtgggg gcgcggaacg
     1921 gcggccggcc gctgagcatc tggtagagga cgctgccgaa gctgaacaga tcggcccggt
     1981 ggtcgagctg ctggcccagg gcctgctcgg gagccatgta catcggcgtt ccggcgatca
     2041 ggccgctttg cgttacgctg gcgtcgtccg ccgtgcgggc caggccgaaa tcggtgagct
     2101 tgacgtggtc cttgatgctg gtttccagca ggatattgcc cggcttgatg tcgcggtgaa
     2161 tgagctgctg ggcgtgggcg gcagccaggc cgtcggcgat ctgcttgccc agccgcagca
     2221 caccttccag gtcgagcggc ccctgcgaat cgagccggtg ctggagcgtc tcgccgggaa
     2281 tgtactccat caccaggtac ggcaggggct gctcttcgac gttgtagatg ctgacgacgt
     2341 tttcgtggcg gatttgagcg gaagcgcggg cctcgcggag gaaccgtttg cgggccggcg
     2401 acgtggaggc catttccggc gagagcacct tgatcgcgac cacccgctgc agcttctcgt
     2461 cgaaggcctt gagcacgatg ccgaacgccc cgcggccgag aacttccagc acttcgtagt
     2521 ggtccagtcg ccccagcgaa ccgggccggg tggaaggctg caggtagccc agggggattt
     2581 cgtcgtcctg ctcgtcgcgg cgctgttcgg cggcgcccac gagcagtgtg gcagccgtcc
     2641 cgaccgcgga atcatcaccc atctgctcca gcgcgggcgt ctggaggaac tggctctggc
     2701 tctcgtgcga ggccagcagc gactgaacgc gggacaaaag ctcggcgttg ccgccgcagc
     2761 tcgactgcaa atgggcctgc cggctggccg gatcgtcgat ttccagggcg gaaagaaaca
     2821 gctcgcgttc ggtcatcacg atgcctccca agcgtcgagt ggggcaatgt ctgctgctcc
     2881 caatgcgccg ttttaccccc gatcgcgcca aatttctcca attttttctc gcttcgcggc
     2941 ggttcgtcgc ggtggcttcc agccgccgaa cgctggagca ggatgctcca gctactggcc
     3001 cgcgatttcc cggtgcagcc aggcccgcgc gaaggcccag aggcggtcgg cggaccgggg
     3061 cgagatcccc agttgttccg ctgcctgcgg gatcgagagg ccgacgaaat atcgcagatg
     3121 aacgagctgc acggcctgcg gatcgatctc cttcaggcgg tcgagcgccg catcgagggc
     3181 gagcaggtct tcgtggattt cgggggcggc gacagcgtcc tcaaccagtt cctcgcgcgc
     3241 caaatcgcca ccccgtttcc ggcgattctt gcggcgggcg ttttcgacca gaatccgccg
     3301 catcgcctcg gccgcggcgg agaagaagtg cccgcgggaa gagaactgag gagtcaggag
     3361 acaggagtca ggagtcaggt tcccagtcgc cgccggctcc ccacggaccc ctgactcctg
     3421 actcctgacc cctggccttg tcacc
//
LOCUS       DASUGB010001225         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_972031, whole genome shotgun sequence.
ACCESSION   DASUGB010001225 DASUGB010000000
VERSION     DASUGB010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972031"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VFV87_23205"
     CDS             complement(<1..1208)
                     /locus_tag="VFV87_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5106756.1"
                     /translation="MNDSTAAPDSYRQALAAVEQTLDKVRRCSEEEKARLRQELAGLR
                     SMHDKLVSGRVEIVVFGEISTGKSALINALVGRDVASVDVRGGWTKEVWHVAWSGAGY
                     VLPGLGSSEVVLIDTPGINEVGGSARGDMAREAAQRADLILFVTDSDLNETEHSSLVQ
                     LATFHKPMIVVLNKIDLYSREQRERLLSVIRNERLKDIVPPEHVVTAAADPREREYVI
                     QAADGTERSEWRKPPPDVADLKSLILESLDREGLSLIALNAALYAADKSDRVSSLRVQ
                     MRNLRAQHVIWSYAALKVIGVGLNPIPIADVAGGTAIDAAMIVTLAAVYGLEITRTRA
                     SQLAASIASAAGLMTAGVLASSALISLLKGVTLGKSTLLTALPQGLAAGFGSYIVGQA
                     AQYYFEHGSS"
     gene            1356..2864
                     /locus_tag="VFV87_23210"
     CDS             1356..2864
                     /locus_tag="VFV87_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106757.1"
                     /translation="MSERILVRGELSVWVHVGRVAGFVALVVGVGIGLAGPPWGWIVA
                     AGGAALWICLEVVAFHARWVRTRLTIQTEGFLVEDRRGSRAIHDAEVIAVALESKRNF
                     SQGQASSVTRTFRLWTERDTEPVVMYGTIKSGQSDPIADLIDRLHDALLARMEQDLAR
                     GLAVGGDGWQMNSAVLSIGRPPNDQQFPLSEITAIDQFDGKLCLWRRGMDEAAAKLAP
                     NGRNVYLLPALLAARMAPQSEKAANDSATGLGRVLFQRKPHTGVVFGLLLGGLVLCIG
                     GLLALVTHPKWPDGMIWFLCLFTVGVILFPVGVWTAFQAFRVHERGVWQGGLFGQKLL
                     RYADVGTFRYQAVDHYHNGVYVGTYLTMDFRPISPELGKRIRYNASVRGGDDDLDTLR
                     DHVSRAIAANMAELYNAGQPVFWTGNLQFLKDGIQYRPSGFLGRKDALILPYEQYGGY
                     DIQQGVFYLFAKGNAKAVCTEQTSTENFFPGFFLLLLLLHTPVDEQEAEAGT"
BASE COUNT          468 a          912 c         1019 g          521 t
ORIGIN      
        1 cagcttgagc cgtgctcgaa atagtactgg gcggcctggc cgacgatata cgacccgaag
       61 ccggcggcga gaccttgcgg cagggcggtg agaagcgtcg atttgccgag cgtgacgcct
      121 ttcaatagcg agatgagggc gcttgaggca aggacgccgg cagtcatcag gccggcggcg
      181 ctggcgatcg acgcggcgag ttgtgaagcg cgggtccgcg tgatctccag gccatagacc
      241 gcggcgagcg tgacgatcat ggcagcatcg atggccgtgc cgcctgccac gtcggcgatt
      301 gggatcgggt tgaggccgac gccgatcacc ttcaaagccg catagctcca gatcacgtgc
      361 tgggcccgca ggttccgcat ctgcacgcgc agcgagctga cgcggtcgct cttgtcggct
      421 gcatacaggg ccgcgttcag ggcgatcagc gatagcccct cccggtcgag cgactccaga
      481 atcagcgact tgaggtccgc cacatccggc ggcggcttgc gccattcgga gcgctctgtg
      541 ccgtcggccg cctggatgac atattcccgc tcgcgcgggt cggccgcggc cgtgacgacg
      601 tgctccggcg gcacgatgtc cttcaaacgc tcgttgcgaa tgaccgacag cagccgctcg
      661 cgctgctcgc gcgagtagag atcgatcttg ttgagcacca cgatcatcgg cttatgaaat
      721 gtggcgagct ggacgaggct gctgtgctcg gtttcgttga ggtccgaatc ggtgacgaag
      781 aggatcaggt cggcccgctg cgccgcttcg cgggccatgt cgccgcgggc ggagccaccg
      841 acctcgttga tgccgggggt gtcgatcagc acgacctccg agctgcccag gccggggagc
      901 acgtatcccg cgccgctcca ggcgacgtgc cagacctcct ttgtccagcc gccgcgcacg
      961 tcgacgctgg ccacgtcgcg gccgacgagg gcgttgatca gggcgctttt gcccgtgctg
     1021 atctcgccga agacgacgat ctcgacgcgc ccgctgacga gcttgtcgtg catcgaccgc
     1081 aggccggcca gttcctgccg caggcgggcc ttttcctcct ccgaacagcg gcggaccttg
     1141 tcgagcgtct gctcgaccgc ggccagagcc tggcggtagg aatcgggggc ggcggtggag
     1201 tcgttcacga agggttcctc tggttcccac gctctgcgag ggaacccaag caccgacgct
     1261 cagcgtcgcg gccgcttagg acgcagagcg tccggagcgt gcgttcccac gcagagcgtg
     1321 ggaacgagta cgcgacacgg ttatcataca cacccatgag cgagcggatt ctggttcgcg
     1381 gtgagctatc ggtctgggtc cacgtgggcc gagtggcggg gtttgtcgcg ctggtcgtgg
     1441 gcgtgggaat tggtctggcc gggccgccct ggggctggat cgtcgcggcg ggcggggcgg
     1501 cgctctggat ctgcctggaa gtggtcgcct tccacgctcg ctgggtgcgg acgcggctga
     1561 cgatccagac cgaaggcttc ctggtcgaag atcgccgcgg cagccgggcg attcatgacg
     1621 ccgaggttat cgcggttgcc ctggagtcga agcggaattt cagccagggg caagcgtcgt
     1681 cggtcacgcg gacgtttcgc ctttggaccg agcgggacac cgagccggtc gtcatgtacg
     1741 gcacgatcaa gtccggacag agcgacccga tcgccgattt gatcgatcgc ctgcacgacg
     1801 ccctgctggc tcgaatggag caggacctgg ctcgcggact ggcggtcggc ggggacggct
     1861 ggcagatgaa tagcgccgtg ctgagcatcg gccggccgcc gaatgaccag cagttcccgc
     1921 tctctgagat cacggcaatc gaccagttcg acggcaagct gtgcctctgg cggcgcggga
     1981 tggacgaagc ggctgccaag ctcgccccca acggccgtaa tgtctatctc ttgccggcgc
     2041 tcttggcagc gcgcatggcg ccgcagagcg aaaaggccgc caacgattcg gcgacgggcc
     2101 ttggccgcgt cctcttccag cgcaagccgc acacgggcgt tgtgtttggc ctgttgctcg
     2161 gcgggctggt gctgtgcatc ggcgggctgc tggctctggt gacacatccc aagtggccgg
     2221 acgggatgat ctggttcctg tgcctgttca cggtgggcgt catcctgttt cccgtgggcg
     2281 tgtggacggc gtttcaggcg ttccgcgttc acgagcgggg cgtgtggcag ggaggtttgt
     2341 tcggtcagaa gctgctgcga tacgcggatg tcggcacgtt ccgctatcag gcggtggacc
     2401 actatcacaa cggcgtgtac gtcggcacgt acctgacgat ggacttccgg ccgatctcgc
     2461 ctgagctggg caagcggatt cgttacaacg ccagcgtccg cggcggcgac gacgacctgg
     2521 atacgctccg cgatcacgtc tcgcgggcga tcgccgcgaa catggccgag ctgtataacg
     2581 ccggccagcc cgtgttctgg accggcaacc tgcagttcct gaaggacggg attcagtacc
     2641 ggccgagcgg ctttttgggc cgcaaagacg cgctgattct gccgtacgag caatacggcg
     2701 gctacgacat ccagcagggc gtgttctatc tgttcgccaa aggcaacgcc aaagccgtct
     2761 gcacggagca gaccagcacc gagaatttct tcccgggttt cttcctgctc ttgctgctgc
     2821 tgcacacgcc cgtcgatgag caggaagccg aggcgggcac gtagggtggg accaactcgc
     2881 gcagcgagcg caggcccacc gtccagcaga attggtgggc
//
LOCUS       DASUGB010001226         6063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1388786, whole genome shotgun sequence.
ACCESSION   DASUGB010001226 DASUGB010000000
VERSION     DASUGB010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388786"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFV87_23215"
     CDS             complement(<1..557)
                     /locus_tag="VFV87_23215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5106758.1"
                     /translation="MLRTVLVGLNGSGFSAAATECALSLAKQHAAGVLGLGVVDVPRL
                     TRPQPVPLGGGAFKDERDAAALKAEREKIAGILASLETQAQAAGVACRTIALEGTPVE
                     LLIRESQRADLLVVGKKHVPHEEWEQSSRTLERLLHQTVRPVLCVPVGGADLTRVLVA
                     YDGSAQSAKALQLFVASGLAAPREI"
     gene            744..817
                     /locus_tag="VFV87_23220"
     tRNA            744..817
                     /locus_tag="VFV87_23220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Val,seq:tac)
     gene            996..3875
                     /locus_tag="VFV87_23225"
     CDS             996..3875
                     /locus_tag="VFV87_23225"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106759.1"
                     /translation="MLSYRGWTGSGVLAACVFVVWTGCETLVVGQQQQPQAARTSAEP
                     IEFLAPPDLLVARTRMAWASALSPDEKWVATGYGHWGSNEAGRVVVWDLETGQPQWEA
                     REPRGVRSLALSPDGMLVASGNFGGEIRLRDSASGKLVRAFRQDRGSVERICFDSQGK
                     RLLTSSNTTNLIRIWDAATGEVLQTIAGHAEVPYWVEFSPDDRLIVSSGRDKTVRIWN
                     AEDGKELHSLPHPGEVSSAIFLPGGKQIASACHDGHIRLWNVSSGELEATLDTTEGGG
                     SCAVAVSRDGKQIAASGYQRIHVWSLPAGELTATLDGHAGLVHGLTFDKDAKRIISSS
                     WDQTVRVWDVPQRLELRTMSLPNGVNDPTGSISAFAYCPERSLIGTVTANNPAAVELR
                     SASDGSLGKSLAGHEVAVLALAFAPGGKWLATAGADNKILVWNLDTGEPEIRLPHHAG
                     PVTAIAWSADGTRLISGSSDHTVRVFDTKTWKELAALEGHTGGVLAVAMLPDGRAVSA
                     GDDALVRVWDVAAKKQLAALAGHEGAVRAIAIAPGGGTIATAGDDLNIHLWDSTTLEQ
                     RASVAGHKQPVLCMAFSPQGKTLASGAAGGGLHVLDPRIGKVRKTFQTHTGRLTGIAF
                     LPDAAGILTAGEDETIRLWHAADPPLSPLVSLAAHGDAAFAVAFSPDGKWLASGGKDA
                     VVALRDPQTGAIKHELKGHKGLVYEVAFSPDSRLLASASSDGTVRVWSPETLKELASF
                     QGWKYKFANIRAVAFAPDSRTLIAGGSDGALRLFDAIDKKPLKDLIGQALPVTDVRFS
                     PDGSLLATSTGDWQQWRVPGELRLWDAKGGEELAALGGHDTEIKRLAFSTSGRRMASA
                     GAGIIVWDPATRKMLGNFRVGSAPTAVTFLDDENLLAVGEFQGGVGIWDISRQTALRR
                     YTGHDKQVAAIAVSPDGQRLATAAHDGTLKLWPLTDSGIPVPAIQ"
     gene            complement(3885..5309)
                     /locus_tag="VFV87_23230"
     CDS             complement(3885..5309)
                     /locus_tag="VFV87_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106760.1"
                     /translation="MGLRHLMQGLGFWTDVFKFSEPSLLRVRLRGDWWLRLAIALGAA
                     GAGTGLLLVLFAINNQPPNPLFTLIGGLVAFVIAAGFLFHGASSAGGRVRVCQEGIQR
                     KRSYAGFGAQMFEEANWPYEAVQQCVIVPGQALGKPFSVLMVGGGGEWELVGIPRKID
                     LAQLAGFLQSRGVAVSSANFVPQEFTKPINLPLSLLVGALGFLMLLVGSGFYAVRAIG
                     PGQPNVAQAGPDLPQPAAFPAPAPGPAPAPLPAPAPPAPAPNALVEQEDAPPSAPANP
                     FAVPPAATERPQPNPGFGNAAGAANPPPIEQPPAPAIPERPAAPLIRATSAKVGRDTS
                     SGGGKGGSPFRAVSPGGEPLVGLAWSSGNWGGNKLVGRLEPLFNATKPSGFQNSVVAK
                     EGYVVGGMSVEGTDFVRAIQLVFVRVNDDGTLDPADSYASDWIGHPAGQPIEAIDSRG
                     GRVIGIHGRRAAILDAVGLVLAEE"
     gene            complement(5381..>6063)
                     /locus_tag="VFV87_23235"
     CDS             complement(5381..>6063)
                     /locus_tag="VFV87_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5106761.1"
                     /translation="IEGVYSMDGDIPDVPRFIDVKRRHKAFLYLDEAHSIGTLGKTGR
                     GIGELFGIPSSEIDIWMGTLSKALGACGGYIAGAAPLVKYIKYTCPGVIYTIGLAPAS
                     AGAALAALRKLKAHPERVTTLAERARDFLSLARERGFYTGDSRGTAIVPVILGNSLHA
                     LAASRLMHERGISVQPILHPAVPESGARLRFFISALHTPEQLRTTADALADVLRGLAP
                     RYVGNRPR"
BASE COUNT         1044 a         2049 c         1985 g          985 t
ORIGIN      
        1 tggatttcgc gcggggccgc caggccgctg gcgacgaaga gctgcagcgc cttggctgat
       61 tgagccgatc cgtcgtaggc cacgaggacg cgcgtcaggt cggcgccgcc gaccggaaca
      121 caaaggaccg gccgcaccgt ctggtgcaac agccgctcca gcgtgcgact ggactgctcc
      181 cattcctcgt gcggtacgtg cttcttccct acgacgagca ggtcggcccg ttgcgattcg
      241 cgaatcaaga gctccaccgg agtcccttcc agcgctatcg tgcggcaagc cacgccagcg
      301 gcttgcgcct gcgtttccag actcgccagg ataccggcga tcttttcacg ttcggccttg
      361 agggccgcgg catcgcgctc gtccttgaag gcgcctcccc ccagcggcac cggctgcggc
      421 cgggtcaaac gcggcacatc aaccacgccc agacccagca cgccggcggc gtgctgtttg
      481 gccagcgaaa gcgcgcactc agtcgccgcc gcgctgaatc cggagccatt caggcccacc
      541 agcactgtcc gcaacatgct tgactccttg cggtgaatca gggcacctct cccattgtag
      601 tgggacaggc atctctgtct gccgccccct gtctcctgtc ccctgtctcc tgtcccctct
      661 ctcctgtccc ctgtcccctg tcccctgtct ccctacctct ccctgtcccc tcatcccctg
      721 gcgtactaca ttacctacag cccgggcgat tagctcagtt ggttagagca cctcgtttac
      781 accgaggggg tcgggggttc gagtccctca tcgcccatct ccctgtctgc gatcgttcgt
      841 gttcgcccat tgcctcccct gcagcggatt gggggattcc cgctcgtcga aatgaagtgg
      901 gaatcgctcg gccccaggga cttcgcagtc ctctgtcgcc ctccgcgccg ttgacagtcc
      961 agacatgccg tcttagtctg cgcggggagg actttatgct cagctatcgc ggatggacgg
     1021 gttcgggagt gctggccgct tgtgttttcg tggtctggac cggttgcgag acgctcgtcg
     1081 ttggccagca gcagcaaccg caggcggcgc gcacgagtgc ggagccaatc gagttcctgg
     1141 cgccgcccga tctgctcgtg gcgcggacgc ggatggcgtg ggcctcggcg ctttcgcccg
     1201 acgaaaagtg ggtcgccacc ggctacgggc actggggatc gaacgaagcg gggcgcgtcg
     1261 tcgtgtggga cctggaaacg ggtcagccgc aatgggaagc ccgcgagccg cgcggcgtgc
     1321 ggagcctggc cctttcgccc gatggaatgc tcgtcgccag cggcaacttc ggcggcgaga
     1381 tcaggctccg cgattcggcc agcggcaagc tcgtccgcgc gtttcgccag gaccgcggct
     1441 ccgtcgagcg gatctgtttc gattcgcagg gcaagcgcct gctcaccagc agcaacacca
     1501 caaatctgat tcgcatctgg gacgccgcga ccggcgaggt gctgcagacg attgccggcc
     1561 acgccgaagt gccctactgg gtcgaattct cgccggacga tcggctgatc gtcagttccg
     1621 gccgcgacaa gaccgtgcgg atctggaacg ccgaagacgg caaagaactg cattcgctgc
     1681 cgcatccggg cgaggtgtcg tcagccatct tcctgccggg cggcaagcaa atcgccagcg
     1741 cctgccacga tgggcacatt cgcctgtgga atgtgtcgag cggcgagctg gaggccacat
     1801 tagatacaac cgaagggggc ggctcgtgcg ctgttgccgt cagccgggac ggcaagcaaa
     1861 tcgccgccag cggctaccag cggattcacg tctggagcct gcccgcgggc gagctgaccg
     1921 ccacgctcga cgggcacgcg gggctggttc acggtctgac ctttgataag gacgccaaac
     1981 ggatcatcag ctcgagctgg gaccagacgg tccgcgtgtg ggacgtgccg cagcgactgg
     2041 aattgcggac gatgtcgttg cccaatggcg tcaacgaccc gaccggctcg atcagtgcct
     2101 tcgcctattg cccggagcga tcgctcatcg gaaccgttac ggcgaataac ccggctgcgg
     2161 tcgagctgcg cagtgccagc gacggctcgc tgggcaaatc gcttgcgggc cacgaagtgg
     2221 ccgtcctggc gctggccttc gctcccggcg gcaagtggct ggcgacggcg ggggccgaca
     2281 ataaaatcct ggtctggaac ctcgatacgg gcgagccgga aatccgcctg ccgcaccacg
     2341 ccggcccggt caccgccatt gcctggtccg cggacggcac acggctgatc agcggcagca
     2401 gcgaccatac ggtgcgcgtg ttcgacacga agacctggaa ggaactagcc gcgctggaag
     2461 ggcatacagg cggggtcttg gccgtcgcta tgctgcccga cggccgggcg gtttcggccg
     2521 gcgacgatgc gctggttcgc gtctgggatg tcgcggccaa gaagcagctg gccgcgctcg
     2581 cagggcacga aggcgccgtc cgcgcgatcg caatcgcgcc cggcggcgga acgatcgcta
     2641 cggcaggcga cgacttgaac attcatctgt gggattcgac tacgctcgag caacgggcga
     2701 gcgtcgccgg ccataagcaa ccagtcctct gcatggcttt ctcgccgcag gggaaaacgc
     2761 tggccagcgg cgcagccggc ggcgggctgc atgtgctcga tccacggatt ggtaaagtcc
     2821 gcaaaacatt tcagacccac acgggccgat tgaccggcat cgcttttctg ccagatgccg
     2881 caggcatctt gacggctggc gaggatgaga ccattcgcct ctggcacgca gccgatccgc
     2941 ctctttcccc actggtgagc ctggcggcgc atggcgacgc ggcctttgcc gtggcctttt
     3001 cgcccgatgg caaatggctg gccagcggcg gcaaggacgc ggtcgtcgcc ctgcgcgatc
     3061 cgcagacggg cgctatcaag cacgagctca aggggcacaa gggactcgtc tatgaggtcg
     3121 ccttctcgcc cgacagccgc ctgttggcca gcgccagcag cgatggaacg gtgcgcgtct
     3181 ggtcccccga gaccctcaag gagctggcct ccttccaagg ctggaaatac aagttcgcca
     3241 acatccgcgc cgtggccttc gcccccgata gccgcacact gatcgcgggc ggatcggacg
     3301 gtgcgctgcg gctcttcgac gcgattgaca agaagccgct caaggacctg atcggccagg
     3361 ccctgccggt gacggatgtg cggttctcgc ccgacggttc gctgctggcc acgagcacgg
     3421 gcgattggca gcagtggcgc gtgccgggcg aattgcggct gtgggacgcc aagggcggtg
     3481 aagaactggc cgcgctgggg ggccacgata cggagatcaa gcgcctcgcg ttcagcacca
     3541 gcggcaggcg catggcttcc gcgggcgccg gcatcatcgt ctgggacccg gccacgcgga
     3601 aaatgctcgg caacttccgc gttgggtccg cccccacggc ggtgaccttc ctggacgatg
     3661 agaatctcct ggctgtgggc gaattccagg gcggcgtcgg catttgggat atcagccgcc
     3721 aaacggccct gcgccgctat acaggccacg acaagcaagt cgccgccatc gccgtcagcc
     3781 cggatggcca gcgcctcgcg accgccgccc acgacggcac actcaagctc tggccgctga
     3841 cggattcggg gattcctgtg ccggcaatcc agtaagggcc agcgtcattc ctccgccagg
     3901 accaggccga cggcgtcgag gatcgcggcc cggcggccgt ggatgccgat cacccggccg
     3961 ccgcggctgt cgatggcttc gatcggctgg ccggcgggat ggccgatcca gtcgctggcg
     4021 taagaatcgg ccgggtcgag cgtgccgtcg tcgttcacgc ggacaaagac gagctggatc
     4081 gcgcggacaa agtcggtccc ttccacgctc atgccgccga ccacatagcc ctccttcgcc
     4141 acgaccgagt tctgaaagcc gctcggcttg gtcgcgttaa acagcggctc caagcgcccc
     4201 acgagcttgt tgccgcccca attgccggac gaccaggcca atcccacaag cggctcgccg
     4261 ccgggactga ccgcgcgaaa cggcgagccg cctttgccgc caccgctgct cgtatcgcgg
     4321 ccgaccttcg cactggtcgc gcgaatcagc ggcgcggccg gacgctcggg gatggccggc
     4381 gctggcggct gctcgattgg cggcgggttc gccgcgccgg cggcatttcc aaagcccgga
     4441 ttcggctgcg ggcgctcggt cgccgccggc gggacggcaa aaggattagc cggagccgaa
     4501 ggaggcgcgt cctcttgttc taccaaggcg ttgggagcgg gcgcaggggg cgccggcgcg
     4561 ggaagaggcg caggagcggg accgggcgcc ggagcgggaa atgctgcagg ctgcggcaag
     4621 tcgggacccg cctgcgcgac attaggctgg cctgggccaa tcgcccgcac ggcgtaaaag
     4681 ccagacccga cgagcaacat cagaaaccct agcgctccaa ccagcagcga caggggcaga
     4741 ttgatcggct tggtaaactc ctgtggaaca aagtttgccg acgaaaccgc tacgccgcgc
     4801 gactgcagaa agccggcaag ctgcgcgaga tcgatcttcc gcggaatgcc gaccagttcc
     4861 cattcgccgc cgccgccgac catcagcacc gagaatggct tgcccagggc ctgccccggc
     4921 acgatcacgc actgttggac cgcttcgtaa ggccagttgg cttcctcgaa catctgcgcg
     4981 ccaaagccgg cgtagctccg cttccgctgg atgccctcct ggcaaacgcg gacgcgtccg
     5041 ccggcgctcg acgcgccgtg gaacagaaag cccgcggcaa tcacgaacgc caccaggccg
     5101 ccgatcaagg taaacagcgg atttggcggc tgattgttga ttgcgaacag cacgagcaac
     5161 aggcccgttc ccgcccccgc cgcaccgagc gcgatcgcca gcctcagcca ccagtccccc
     5221 cgcagccgca cgcgcaatag cgacggctcc gaaaacttga agacatcggt ccagaatccc
     5281 aaaccctgca tcagatggcg caagcccata cgacaacctc cgcggcgatg gacccattgt
     5341 caggcgcacc aataggcgat tcaacaagcc acgtggtact tcatctcggc cggtttccca
     5401 cgtaccgcgg agcgagccca cggagaacgt ctgccagcgc gtcggcagtc gtccgcagtt
     5461 gctccggcgt gtgcagcgcg ctgatgaaga accgcagccg ggcgccggat tcggggacgg
     5521 ccgggtgcag gatcggctga acgctgatcc cccgctcgtg catcagtcgg gaggcagcga
     5581 gggcatgcag cgagttgccg agtatcacgg ggacaatcgc cgtcccgcgg ctgtcccccg
     5641 tgtagaagcc gcgctccctg gcaagggaaa ggaaatcgcg ggcccgctcc gccagggtcg
     5701 taacgcgctc gggatgagct tttagcttgc gaagcgccgc caacgcggca ccggcgctgg
     5761 cgggcgccag gccaatggtg tagatcacgc cggggcaggt gtacttgatg tacttgacca
     5821 gcggagcagc ccccgcaatg tagcccccgc aggccccaag ggccttgctg agcgtgccca
     5881 tccagatgtc gatctcggag ctcggaatgc caaatagctc tccgatcccg cggccggttt
     5941 tgcccagcgt gccgatcgag tgggcttcgt cgaggtacaa gaaagctttg tgtcggcgtt
     6001 tgacgtcgat aaatcgcggc acatcgggga tgtcgccgtc catgctatag acgccttcga
     6061 tga
//
LOCUS       DASUGB010001227         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1110514, whole genome shotgun sequence.
ACCESSION   DASUGB010001227 DASUGB010000000
VERSION     DASUGB010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110514"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1218
                     /locus_tag="VFV87_23240"
     CDS             145..1218
                     /locus_tag="VFV87_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5106762.1"
                     /translation="MAALQTTSVSTTQATSGGNGSEDMAVIARLREARDRVKQEMAKV
                     VVGQNEIIDSLLIGLLCRGHILMLGVPGLGKTLMGRTLARTLDMEFRRVQFTPDLMPA
                     DITGTDVMEEDQATGKHRVSFMQGPVFTNFLLADEINRTPPKTQAALLQAMQEGEVSV
                     GRETYKLKPPFFVVATQNPIEMEGTYPLPEAQLDRFMFNLKVQYPTVDEEVQIIRGTT
                     SKLTAHADKVLGSDEVLRLQEIVRGVPIADSVIRYTARLVAATRPGASAAAAGAKNLE
                     AIHKYLSYGASPRASQYLVLGAKGRAILAGKYHVDFADIQAVAAPVLRHRLVLNFHAR
                     ADNVDSDALVERLLKSVSVEENK"
     gene            1218..2078
                     /locus_tag="VFV87_23245"
     CDS             1218..2078
                     /locus_tag="VFV87_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106763.1"
                     /translation="MTLCREKVLSAGRTWLLVAAVALGPLGAARGEDSAERRNEYLKQ
                     MNALAKATDVRLAESDDPARLVESPVFRYDDQPRRFLDATMWIWTDGVRPVACQKIEA
                     RRHFDTDKPLWGYCFTSTSAEPIAARWSGDRRWRSTEAGIEWQPVPGAGAPRATSAAR
                     RLQAREIARGFACRMLINPRTEETAAMRLLTTPIYEYADPESKLLVGAVFGFEVNGTN
                     PDLLVLVEARGDADKPAWHFAPARMTTGGLKLKYGDKAVWECPFVEPTTGPYSNWLFF
                     STPREPLAGE"
     gene            2095..2946
                     /locus_tag="VFV87_23250"
     CDS             2095..2946
                     /locus_tag="VFV87_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106764.1"
                     /translation="MIVCYRQFACLLLLSSMLSAAAAEEPADSSSAEQALRTRWQEVY
                     GEIAASIEMSDGRSPLRLHDNPLLSYSNPVRSTDQHGSIFLWTDRGRPAVFGSIWSAE
                     NRTDHSLRNITHEFHSLSTSQNVQAIRDGRVQWSAGEPGIEWQTLADAPAPATTRSAR
                     LVQMRDLARRLTAKITAPEETELRLMPRPLYRYPEDTAGAMDGALLVFVLATDPELIL
                     LIEADDSDKPVYRTAIARFGNVSMSVAYGDRQIWSCDRGTPGRSEGKYYLHWRIDQRP
                     ANLTPAP"
     gene            2962..>3590
                     /locus_tag="VFV87_23255"
     CDS             2962..>3590
                     /locus_tag="VFV87_23255"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5106765.1"
                     /translation="MSFDPRTLASPAFFSRLDNLELRARGNVEGFMHGLHRSPFVGFS
                     VEFASHREYAQGDDLRHVNWKLFARSNRLYVKQFDAETNMNLYLLLDVSGSMDCRSKG
                     ISKLEYGSALAAALSHLALKQHDAVGLVLYADEVLAHLPPKARPHQLNEILRIIGNTK
                     PNRSSNTARALPQAAELCNHRGIVVILSDLFDDLDAIMKGIERLRFQKH"
BASE COUNT          670 a         1195 c         1116 g          609 t
ORIGIN      
        1 ctggtctggg acgaattcct aggcgcgctc tggactttcg aagaactcga tgccgaccac
       61 gacgagctgc tggatcccca agaggcgagc aaggccaaag cttccgcgga gaactgattc
      121 attttcacga aaacggaccc atccatggct gcattgcaaa cgacttccgt gagcacaacc
      181 caggcaacca gcggcggcaa tggctcggaa gatatggcgg tcatcgcccg cctccgcgaa
      241 gcccgcgacc gcgtgaagca agagatggcc aaggtcgtcg tcgggcagaa cgagatcatc
      301 gattcgctcc tgatcggcct cctctgtcgc gggcacatcc tgatgctcgg agtgccgggt
      361 ctgggaaaga cgctgatggg gcgcacgctc gcccgcacgc tcgatatgga gttccgccgc
      421 gtgcagttca cacccgacct gatgccggcc gatatcaccg gcaccgacgt gatggaggag
      481 gaccaggcaa ccggcaagca tcgtgtgtcg ttcatgcagg ggccggtctt caccaatttt
      541 ctgctggcgg acgagatcaa ccgtacgccg cccaagacgc aggctgcact gttgcaggcc
      601 atgcaggaag gcgaagtttc agtcggccgc gagacctaca agctcaagcc gccgttcttc
      661 gtcgtggcca cacagaaccc gatcgaaatg gaaggcacct acccgctgcc cgaggcgcag
      721 ctcgaccgat tcatgttcaa tctgaaggtg caatacccga cggtggatga ggaagtgcag
      781 atcattcgcg gcacgacgag caaattgacg gctcatgccg acaaggtgct gggctcggac
      841 gaagtcctgc gactgcaaga gatcgtccgc ggcgtaccca tcgccgatag cgtgatccgc
      901 tacacggccc gcctcgttgc cgccacgcga cccggcgcga gcgcagccgc cgcgggcgcg
      961 aaaaacctgg aagcgatcca caagtacctg tcctacggcg ccagcccgcg cgcttcgcaa
     1021 tatctggtgc tgggcgcgaa aggccgtgcc attctcgccg gcaagtacca cgtcgatttc
     1081 gccgacatcc aggcagtcgc cgcgccagtc ctgcggcatc gcctcgtcct caacttccac
     1141 gcccgcgccg acaacgtcga ttccgacgcc ctcgtcgagc ggctgctgaa gagcgtctcg
     1201 gtggaggaga ataagtagtg actttgtgcc gcgaaaaggt gttgagtgct ggccgaacgt
     1261 ggctgctggt tgcggcggtc gctctgggcc cgctgggtgc ggcgcgcggc gaagactctg
     1321 ccgagcgacg gaacgagtat ctcaagcaaa tgaacgccct ggctaaggcc acggacgtgc
     1381 ggctggctga gtccgatgat ccggccaggc tcgtcgaaag ccccgtcttc cgctacgacg
     1441 atcagccgcg gcggttcctg gatgccacga tgtggatctg gaccgatggc gtgcggccgg
     1501 tcgcctgtca gaaaatcgag gcgcggcggc acttcgatac ggacaagccg ctgtggggct
     1561 attgcttcac ttcgacttcc gccgaaccca tcgcggcccg ctggtcgggc gaccgccggt
     1621 ggcgttcgac ggaggccgga atcgagtggc agcccgtccc gggcgcaggt gccccgcgcg
     1681 cgacgtccgc cgcccgccgc ctgcaggccc gcgaaatcgc ccgcggtttc gcgtgccgca
     1741 tgctgatcaa tccgcgaacg gaagagacgg ctgccatgcg gctgctcaca acgccgattt
     1801 acgagtacgc agatccggaa tcgaaactgc tcgtcggcgc ggtcttcgga ttcgaagtca
     1861 acggcacgaa ccccgatctg ctcgtgctgg tcgaggcccg cggcgacgcg gacaagccgg
     1921 cgtggcactt tgcgccggcc cgaatgacca cgggcggcct caagctgaaa tacggcgaca
     1981 aggccgtctg ggagtgtccg ttcgtcgagc cgacgaccgg tccctattcc aattggctgt
     2041 ttttctcgac tccccgcgaa ccgttggccg gcgagtagcc gcatcggctg aactatgatc
     2101 gtctgctacc gacaattcgc ttgcctgctg ctgctttcga gcatgcttag cgcggctgct
     2161 gccgaagagc ctgccgattc gtcgtcggcc gaacaggccc tgcgcacgcg gtggcaggag
     2221 gtctacggcg agatcgcagc gagcatcgaa atgtccgatg gcaggtcgcc gctaaggctc
     2281 cacgacaacc cgcttctttc ttacagcaac ccagtgcgct cgactgacca gcatggttcg
     2341 atcttcctgt ggaccgatcg ggggcgccca gcggtcttcg gcagcatctg gtcggctgag
     2401 aaccgcaccg accacagcct ccgcaatatc acgcacgaat tccattcgct ttcgaccagc
     2461 cagaacgttc aggcgatccg ggacggacgc gtgcagtggt cagccggcga gccgggcatc
     2521 gagtggcaga ctctggcgga cgcgcccgct ccagccacca cgcgttccgc gcgcctcgtc
     2581 cagatgcggg atctcgcccg ccggctgacg gccaagatca ccgcgccgga ggagaccgag
     2641 ttgcggctga tgccccggcc tctctatcgt tatcccgagg acacggccgg agcaatggat
     2701 ggcgcattgt tggtgttcgt cctggcaacc gatcccgagc tgattctgct gatcgaagcg
     2761 gacgacagcg acaagcccgt ttaccgcaca gccattgccc gatttggcaa cgtgtcgatg
     2821 tcggtcgctt acggcgaccg acaaatctgg tcgtgcgacc gcggcacgcc cggccgcagc
     2881 gaaggcaagt attacctgca ctggcgcatc gaccagcgcc ccgcgaatct gactcctgcc
     2941 ccctgaatcc tgaccctgac catgtccttc gacccccgca ccctcgccag ccccgcgttc
     3001 ttctcgcggc tcgacaacct cgagctgcgg gcccgcggca acgtcgaggg gttcatgcac
     3061 ggcctgcacc gcagcccgtt cgtggggttc agcgtcgaat tcgcctcgca ccgcgaatat
     3121 gcccaggggg acgacctgcg gcacgtcaac tggaagctgt tcgcccggtc gaaccggctg
     3181 tacgtcaagc agtttgacgc cgagacgaac atgaacctct acctgctcct ggacgtgagc
     3241 gggtcgatgg actgccgcag caaggggatc agcaagctgg agtacggctc ggctctggcg
     3301 gcggccctct cgcacctggc cctcaagcag cacgacgccg tcggccttgt gctgtatgcc
     3361 gacgaggtcc tcgcccacct gccgccgaag gctcggccgc accagctcaa cgagatcctc
     3421 cggatcatcg gcaacacgaa gcctaaccgg tcgagcaaca cggcccgcgc gttgccccag
     3481 gcagccgagc tgtgcaatca ccgtggcatt gtcgtgatcc tgagcgacct gttcgacgat
     3541 ctcgatgcga tcatgaaggg gatcgaacgg ctgcggttcc agaagcacga
//
LOCUS       DASUGB010001228         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1805801, whole genome shotgun sequence.
ACCESSION   DASUGB010001228 DASUGB010000000
VERSION     DASUGB010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805801"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFV87_23260"
     CDS             complement(<1..902)
                     /locus_tag="VFV87_23260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106766.1"
                     /translation="MASQLKCSKCQTLIPSPASPEVEHVQCPGCGARFRIRRSASSTP
                     APVATAAAQPASAKTAPAPLSDPDDEYRLAAPTPAPQPLPRPPVAAIDPLGRPAGSQA
                     LTAAREPASGRFDLRILIGAGMGGGALLLVLVIGVVAWLVMSGGGNGPGQNGVVGNPP
                     GGSGKLSTTAVVFDTADPNIKPDPPGDVSQYRQTTEGPPQVWTAIAVLPPATTQSLVG
                     GASMGNWDGEPDPAPRATPLPVETAFQFTTRPIFAAQDGPFAVEAWHRDRADLLPPDE
                     PLGQDDQSRGLGAFPVYDLRTGEAV"
     gene            complement(1101..1976)
                     /locus_tag="VFV87_23265"
     CDS             complement(1101..1976)
                     /locus_tag="VFV87_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5106767.1"
                     /translation="MKAFARMCCAALFAVGLAVPALAQPMPYSEPLGIEELGESAFES
                     EGEEEEEDEIETDRDSFTPATTTTPWGCMIVESAYSFIDNKNVPETHSFPELLLRYGA
                     TDWLELRLGWNFEIGGASNPISGSVPDDFESEPGIEEEHRLLYGLKAWLTGQDGWRPQ
                     SVVILQGFTPTGGESNNTEMSATYAFGWTLAKRWVWDTGLRFSTAGETDDDFNVWAPS
                     TVLKFPLGERWKAHVEYFEIFTDGREIETVQHYFSPGAHYLVTPNLEVGARFGWGLNE
                     QAANFFSNVGFGWRY"
     gene            2348..>3742
                     /locus_tag="VFV87_23270"
     CDS             2348..>3742
                     /locus_tag="VFV87_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5106768.1"
                     /translation="MLRISVPASLVLPSLLTLLLLPAAASAQQKPNILIIWGDDIGIW
                     NVSFNSRGMMGYQTPNIDRIAKEGLSFTDYYGQQSCTAGRAAFIGGNVPVRTGMTKVG
                     LPGAKEGWQKTDVTMATVLKSLGYATGQFGKNHQGDLDEHLPTMHGFDEFFGNLYHLN
                     AQEEPENEDYPRNLKLPNGKTFLEQYGPRDILLCKADGKGGQTIEAKGQLTKKRMETI
                     DEETLAAAKDFITRQNKAGQPFFCWWNATRMHLRTHVKEANRGKSGQDEYSDGMVEHD
                     GQVGELLKLIDDLKLANDTIVFYSTDNGPHYNTWPDAGTTPFRSEKNSNWEGAYRVPA
                     FVRWPGKFPAGITKNGIVSHEDWLPTFAAAAGAPDIVEKIKAGVELNGRKYKNYIDGY
                     NQLDYLSGKAKDSPRKEFIYVNDDGQIVAMRVNDWKAVFLENRAHAFEVWREPFVELR
                     VPLLFNLRRDPFEKA"
BASE COUNT          720 a         1175 c         1192 g          655 t
ORIGIN      
        1 actgcctcgc cggttcgcag gtcgtacacc ggaaaagccc cgagtcctcg tgactgatcg
       61 tcctgaccca gcggctcgtc gggcggcagc aggtcggctc ggtcgcggtg ccaggcttcg
      121 acggcaaagg gaccgtcctg cgctgcgaag ataggccgcg tcgtgaattg aaatgcggtt
      181 tcaaccggca gcggcgttgc gcgcggggct ggatcgggct ctccgtccca gtttcccatt
      241 gaagcgcccc cgacgagcga ttgagttgtt gcgggcggca ggacggcgat cgcggtccag
      301 acctgtggcg gcccttccgt tgtttggcgg tactgcgaaa cgtcgcccgg cgggtccggc
      361 ttgatgttgg gatcggccgt gtcgaagacg accgccgttg ttgagagctt gccgctgccg
      421 cccggtggat tgcccacaac tccgttttga cccggcccat taccgccgcc actcatgacg
      481 agccacgcga caacgccaat caccaggacc agcagcagtg ctccgccgcc catgccggca
      541 ccgatcagga tgcgcaggtc gaaccgtccg ctcgccggct ccctcgccgc cgtcagcgcc
      601 tggctgccgg ccggcctgcc cagggggtca atggccgcga cgggtggacg cggcagcggc
      661 tgtggtgccg gagtgggtgc cgccaggcga tactcatcgt caggatcgct caacggagcc
      721 ggcgctgttt tggccgacgc cggctgggct gcggctgttg caacgggcgc cggcgtcgaa
      781 gatgcgctgc ggcgaattct gaaccgcgca ccacaacccg ggcactgcac gtgctcgacc
      841 tcgggcgaag cgggggatgg aatgagcgtc tggcacttcg aacacttgag ctggctggcc
      901 ataaaatcct cgcccccaaa tcgagccgaa gaattcgccg cggtcagcgc ccaatcacgt
      961 cagccgccaa gcccggctgt catcatcacg cccagtatgc caagtatggt aaggatcgcg
     1021 ttgcaagact gcaatctgca caagcccgac gggcaaattc tcagtcggcg ttgaacagcc
     1081 ctagcaagaa aaagcgggcg ctaatatcgc cagccgaagc cgacgttgct gaagaagttg
     1141 gcggcctgtt cgttgaggcc ccagccgaag cgggcgccga cttcgaggtt cggcgtgacc
     1201 agatagtggg cgccggggct gaaatagtgc tggacggttt cgatctcgcg accgtcggtg
     1261 aagatctcga agtattcgac gtgggccttc caccgctcgc ccagcgggaa cttcagcacg
     1321 gtggagggcg cccagacgtt gaaatcgtcg tcggtttcgc ccgctgtgga gaaccgcagg
     1381 ccggtgtccc agacccagcg ttttgccagc gtccagccaa aggcgtacgt ggccgacatt
     1441 tccgtgttgt tgctctcgcc gccggtgggc gtgaaacctt ggagaatgac cacactttgc
     1501 ggccgccagc cgtcctgtcc ggtcagccag gccttgaggc cgtacagcag tcggtgttct
     1561 tcttcgatcc ccggctcaga ctcgaagtcg tctggcacgc tgccggagat ggggttgctc
     1621 gcgccgccga tctcgaagtt ccagccgagc cgcagttcca gccagtcggt cgcgccgtag
     1681 cgcagcagca gctcaggaaa gctgtgcgtc tctgggacgt tcttgttgtc gatgaacgag
     1741 taggcggact caacgatcat gcatccccag ggcgtggtcg tggtcgccgg cgtgaacgag
     1801 tcgcggtcgg tctcgatttc gtcttcctct tcttcctcgc cttccgactc gaacgccgat
     1861 tcgcccaact cctcgatgcc cagcggctca gagtacggca tcggctgggc tagcgcgggc
     1921 acggcaagtc ccaccgcgaa gagcgccgcg cagcacatcc ttgcaaatgc cttcatgacc
     1981 ggccggctcc tggcggcgag agggggcgac cttgccctac caccccctat cggcaagagc
     2041 tgcaccggag cccgccgacg gtgccggcgg tagcgatttt aagggaattg aaagagcaga
     2101 tggaaacagc ggtgggagcc gttttggcga gccggtgacc caacgcgtct gctggacata
     2161 caggccggcc gtgccataat gcgcgcctat tcgcgtctgc acggaaaacc aggcgccgtt
     2221 cgctttgggg cggcaagagg gcccttgccg cagggaaaac cgccgcgttc ctcgcggaga
     2281 ttccgcgccc ggctggatcg aggacgtcag ttgtgaatcg tcctatcgaa cttcaggagt
     2341 tacgtccatg ttgcgcatct cggttcctgc gtctctggta ctaccgtcac tgttgaccct
     2401 tctgctgctg cctgccgccg ccagcgccca gcaaaagccg aacatcctca tcatctgggg
     2461 cgacgatatc ggcatctgga acgtcagctt caacagtcgc gggatgatgg gctatcaaac
     2521 gcccaacatt gaccgcatcg ccaaggaagg cctgtcgttc actgattact acggccagca
     2581 aagctgcacg gccggacggg ccgcgttcat cggcggcaat gtgccggtcc gcaccggcat
     2641 gacgaaggtc ggcctgcccg gcgcgaagga aggctggcaa aagaccgacg tgacgatggc
     2701 cacggtcctc aagagccttg gctacgccac ggggcagttc ggcaagaatc atcagggcga
     2761 tctcgatgag cacctgccga cgatgcacgg gttcgacgag ttcttcggga atctctatca
     2821 cctgaacgcc caggaagagc cggagaacga ggactatccg cgaaacctca agctgcccaa
     2881 cggcaagacg ttcctcgaac aatacggccc ccgagacatc ctgctctgca aggcggacgg
     2941 caagggcgga cagacgattg aggccaaggg acagctcacg aagaagcgga tggaaacgat
     3001 cgacgaagag acgctggccg ccgccaagga tttcatcacg cggcagaaca aagctggcca
     3061 gccgttcttc tgctggtgga acgcgacgcg gatgcacctg cgcacgcacg tcaaggaggc
     3121 caaccgcggc aagtcgggcc aggatgaata ctctgacggc atggttgagc acgacggtca
     3181 ggtgggcgaa ttgctcaagc tgatcgacga cttgaagctg gccaatgaca caatcgtctt
     3241 ttattcaacc gataatggcc cccattacaa cacgtggcct gacgccggca ccacgccgtt
     3301 ccgcagcgag aagaacagca actgggaagg cgcctaccgc gtgcccgcat tcgtccgctg
     3361 gccgggcaag tttcccgcgg gcatcaccaa gaacgggatc gtttcgcacg aagattggct
     3421 gccgacgttc gccgccgccg ccggcgcgcc tgatatcgtc gagaagatca aggcgggcgt
     3481 cgagctgaac ggccgcaagt acaagaatta catcgatggc tacaaccagc tcgactatct
     3541 gagcggcaag gccaaggatt ctccgcggaa ggagttcatt tacgttaacg acgacggcca
     3601 gattgtggcg atgcgtgtta acgattggaa ggcggtgttt ctggaaaacc gggcccacgc
     3661 gttcgaggtc tggcgcgagc cgttcgtcga gctgcgcgtg ccgctgctct tcaacctgcg
     3721 gcgcgacccg ttcgagaagg cc
//
LOCUS       DASUGB010001229         5066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_415635, whole genome shotgun sequence.
ACCESSION   DASUGB010001229 DASUGB010000000
VERSION     DASUGB010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415635"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFV87_23275"
     CDS             <1..472
                     /locus_tag="VFV87_23275"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX5106769.1"
                     /translation="NVHNVIATNVLGSLHARLRGRPCQPFNSDTKIRVRLPTHLRFYY
                     PDASVVCQSNPLDDSFQDHPVVIVEVLSKATRRLDEGEKKDAYLTIPTLAVYLLVEQE
                     SASVTVFRRTEQGFVGELYNGQAAVIPLPEIETALPLAEIYSRVEFVPEPDDEE"
     gene            complement(570..1739)
                     /locus_tag="VFV87_23280"
     CDS             complement(570..1739)
                     /locus_tag="VFV87_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-directed GTPase
                     activating protein isoform b"
                     /protein_id="HEX5106770.1"
                     /translation="MKRDLLAGAAGGLLLGFLAVGMAGCGGATGTTPETAVTNASGAA
                     VDSSGLPSDDEILRQIDEALEYTYEKRRLSVNEQAAWQIIHGALAFKREFLVSDGTQD
                     VSAVNYVLAGGKMKGWNLRRGDLLDESTMRYGVKALVDEDKQGQGHCDQWMGYLSDCH
                     LPLDETVMVEGQPHTIQDWIDQIQLDVVENPTDEYSWTLMCLTAYYPTDYTWKAKDGS
                     EWSIEKLVEIELGHDLDQSACGGTHRMTGMTMAYNRRLAAGKPMEGVWAQLGERIKEC
                     QRKAREYQNEDGSLSSNYFARPGKAADLALMVGSAGHVMEFLATSMDKEELQQEWVKR
                     AVLDVCKVFRKTKAVDVECGALFHAAHGLVLYREKVYGPRTFGKTEPAAVTAAKP"
     gene            2103..2885
                     /locus_tag="VFV87_23285"
     CDS             2103..2885
                     /locus_tag="VFV87_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5106771.1"
                     /translation="MLARKPIFPNVLEMNFQAGQVLGCCVYLVFDGADWILIDIGLED
                     TVDEIVELIRQMDFPLSKCKMLIGTHADVDHIQGLAKAKQLLKAPVYAHPRAIAPLEA
                     GDKLRTFAQIEAQKIDMAMPPVKIDGKLEDGDTIEVGKLKLEVWLTPGHTDSQLSFRL
                     GDLLFSGDNIYRDGCVGAIDAHHGSDIPSFIQSLKRIRASDVKWLLPSHGPYFRKDDK
                     LLDKTIARLESYLHMADFGTCAIDWPLLDQWEQELAEGRRPG"
     gene            2926..3519
                     /locus_tag="VFV87_23290"
     CDS             2926..3519
                     /locus_tag="VFV87_23290"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEX5106772.1"
                     /translation="MADEQRELDQRWWDARLAALEGVLGKADEIMGHSPVPFIMGAEC
                     GGAADILYFRKHIPGVISVTCELIGGDDTGVGAQVQNIMGEFELMICERETAEWGGRA
                     IGRLAYYTFEHRLNPFDNMEIYRAVPKNSTIYGFLFFDYARFEVLGNRAGLLLCVGIT
                     EDELNACRTGRRQEVENALKAANVYPYTDLFRSSVLA"
     gene            3831..4895
                     /gene="floA"
                     /locus_tag="VFV87_23295"
     CDS             3831..4895
                     /gene="floA"
                     /locus_tag="VFV87_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909067.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEX5106773.1"
                     /translation="MYLLAQGGGLENMLGIAAVIGAVLVLFVLLIIVAVFANYFRLWI
                     QSFLTGAGIRIWDLIGMTFRKVNKDVIVKSKIMAVQAGLDESEGITSKSLEAHYLAGG
                     NVPLVIRSLIAASKAKIIRLSFREATAIDLAGRNVLEAVQTSVYPKVIDCPPRGTQAL
                     EAVARNGIQLRVRARVTVRANLQRVIGGATEETIIARVGEGIVSAIGKADDHMQVLEN
                     PDQISKEVLDRQLDANTAFEIVSIDIADIDVGENIGARLRADQAEADTRVARARAEGR
                     RAAAVAAEQENFAHIEESRAKLVEAEAEVPKAMADSFRSGRLGILDYYKLRNVQADTE
                     MRRAIAGAGSTGGSAGQGPR"
BASE COUNT          988 a         1600 c         1503 g          975 t
ORIGIN      
        1 aaatgtgcac aacgtgattg cgaccaacgt tcttggctcg ctccatgccc gcctccgcgg
       61 gcgaccctgc cagcctttca actccgatac gaaaatccgc gtccgcctgc cgacgcactt
      121 gcggttctac tatcccgatg cctccgtcgt ctgccaatcc aatccactgg acgattcgtt
      181 tcaggaccat ccggttgtga tcgtcgaagt gctttccaag gccacgcgtc ggctggatga
      241 aggcgagaag aaggacgcct acctgacgat tccgacgctc gctgtttatc tgctcgttga
      301 acaggagtcc gcttctgtca cggtattccg ccgcaccgag cagggatttg ttggcgaact
      361 ctacaacggt caagccgcgg tgattccgct gccggaaatc gaaacggccc tgccgctggc
      421 ggagatctac agccgcgtcg agtttgttcc cgaaccggac gacgaagagt aggctgtagg
      481 gtgcgtgagg ccgccgcggc cgaaatgcac cattgccggc ggttcacggc gcgcgatggt
      541 gcattgcgct tcgctgatgc accctacgct tacggcttag ccgcggtgac ggcagcgggt
      601 tcagtcttgc cgaaggttcg cgggccatag accttttcgc ggtagagcac gaggccgtgg
      661 gcggcgtgga agagggcgcc gcattcgaca tccacggcct tggtttttcg gaagaccttg
      721 cagacatcga gcacggcccg cttgacccat tcctgctgca gctcttcctt gtccatcgac
      781 gtggcgagga attccatcac gtgacccgcc gatccaacca tcagggccag gtcagccgct
      841 ttgccggggc gggcgaagta gttgctcgac agcgagccgt cctcgttttg atactcgcgg
      901 gccttccgct ggcactcctt gatccgctcg ccgagctgcg cccagacgcc ttccatcggc
      961 ttgccggcgg cgaggcggcg gttgtaagcc atcgtcatgc cggtcatgcg gtgcgtgcca
     1021 ccgcaggcgc tttggtcgag atcgtgaccc agctcgattt cgacgagctt ttcgatgctc
     1081 cactcgctgc cgtctttggc cttccaggtg taatcggtcg ggtaataggc ggtcaggcac
     1141 atcagcgtcc agctatattc gtcggtcggg ttctccacca cgtcgagttg gatctggtcg
     1201 atccagtcct ggatcgtatg cggctggccc tcgaccatca ccgtctcgtc gagcggcagg
     1261 tggcaatcgc tgagatagcc catccactgg tcgcagtgcc cctgaccttg cttgtcttca
     1321 tcgacgagag ccttcacgcc gtaccgcatg gtcgattcgt caagcaaatc gccccgccgc
     1381 aggttccagc ccttcatctt gccgccggcc agcacatagt tgacagccga aacgtcctgc
     1441 gtgccgtcgc tcacgagaaa ctcgcgcttg aaagccaatg cgccgtgaat gatctgccat
     1501 gccgcttgct cgttgaccga gaggcggcgc ttttcgtacg tgtattcgag ggcctcatcg
     1561 atctggcgca ggatctcgtc gtcgctgggc aggccagatg aatcgactgc ggcgccgctg
     1621 gcgttggtga ctgccgtttc gggcgtcgtc ccggtcgctc caccgcaacc ggccatgccc
     1681 acagcaagaa agcccaacaa caagccgccg gcagcgccgg ccagaagatc gcgtttcatc
     1741 agagggagtc cagccgcgag ccgcgctccg cgcgcaccca catattggag gggccggccg
     1801 gttccctagc cagcgaaggg gaagttggca aatatacccc acgattgccg caccggccag
     1861 cagactgaag cgactgcgca aaatggataa ttctgaaaga gattgccgtc attccaacgc
     1921 cgcgggcaat ctcgtatcag tttccaacgc cagcgccgtg agactcgctc tcaataaccg
     1981 caatcgccac aacaatttgc gcgcgatccg ccccgcgccg gttgctggcc ctaaccctcg
     2041 cccctacaat cgcgcacgcc tcttctgtct gacccctgac ccctgactcc cgacccctgg
     2101 caatgctcgc ccgcaagccg atcttcccga acgtcctcga aatgaacttc caggccgggc
     2161 aagtcctggg ctgctgcgtc tatctggtgt tcgacggcgc cgattggatt ctgatcgaca
     2221 tcggccttga ggacacggtc gatgagatcg tcgagctgat ccggcagatg gatttcccgc
     2281 tgagcaagtg caaaatgctg atcggcacgc acgccgacgt cgatcacatc caagggctcg
     2341 ccaaggccaa gcaactgctc aaagcccccg tctatgccca tccgcgggcg attgccccgc
     2401 tcgaagccgg cgacaagctc cgcacattcg cccagatcga ggcccaaaag atcgacatgg
     2461 cgatgccgcc ggtcaagatc gacggcaaac tggaagacgg cgacacgatc gaggtcggca
     2521 agctcaagct cgaagtctgg ctgacccctg gccatacgga tagccagctc agcttccgcc
     2581 tgggcgatct gctcttctcg ggcgacaaca tctaccgcga cggctgcgtc ggcgcgatcg
     2641 acgcccacca cggcagcgat attcccagct tcatccagtc gctgaaacgc attcgcgcca
     2701 gcgacgtcaa atggctcctc cccagccacg gcccctactt ccgcaaagac gacaagctcc
     2761 tcgacaaaac gatcgcccgc ctcgaaagct acctccacat ggccgacttc ggcacctgcg
     2821 ccatcgactg gccgctctta gaccagtggg agcaggaatt ggccgagggg aggcggccgg
     2881 ggtgaatttc attccctagt ttcacagctc tatttgtatc gacagatggc cgacgagcag
     2941 cgagagttag accaacggtg gtgggatgca cggcttgccg ccttagaggg cgtcttgggc
     3001 aaggcggatg aaatcatggg acactcgccg gttcccttca tcatgggcgc ggaatgcggc
     3061 ggagcggcag atatcctcta cttccgtaag cacatccccg gcgtgatttc ggtgacgtgc
     3121 gagttgatcg ggggcgacga taccggcgta ggtgctcaag tgcagaatat catgggcgag
     3181 tttgaactga tgatttgcga acgagaaacc gctgaatggg gagggagggc aatcggccgc
     3241 ctagcctatt acacattcga acatcggttg aacccattcg acaatatgga aatctacaga
     3301 gccgttccca agaattcgac tatctacggc ttcttgtttt tcgactacgc aagatttgag
     3361 gtgctaggca atcgtgcggg actactgtta tgcgtcggga tcaccgaaga cgaactaaat
     3421 gcttgtcgta caggacgccg gcaggaagtc gagaacgcgc tcaaagcagc gaacgtctat
     3481 ccgtacaccg atttgtttcg cagttcggta ttggcctagg aacgctcaca ccatctgctc
     3541 ccaccgattg aactcgcacg caccgcgaaa cgtggcggcc aggtctacat tggcggcaag
     3601 tccttcttcg atctcccagt aaatctccgg cagcggctcg cccgcattcc ggttgtggcc
     3661 tggacgcagc gatcgcgcaa aaggcccgcg ctctcccttc ggcagcagct tcccctgccc
     3721 cgaccgttcg tgcagagctt gcttgacaac tgccggcgcg tgggatacga cactgactga
     3781 ctcggcggtg agccggtcgg ctcgcgcttc actccaaata cccgacggtt atgtatctac
     3841 tcgcacaggg cggcggtctg gaaaacatgc tcggtatcgc ggcagtcatc ggcgcggtgc
     3901 tggtgctgtt cgtcctgctg atcatcgtcg ccgtttttgc caactatttc cgcctctgga
     3961 ttcagtcctt tctgaccggc gccggcatcc ggatctggga cctgatcggc atgacgttcc
     4021 gcaaagtgaa caaggacgtc atcgtcaaga gcaagatcat ggcggtgcag gcgggcctgg
     4081 acgaatcgga agggattacc agcaagtcgc tggaggccca ttatctggcc ggcggcaacg
     4141 tgcccctggt gatccgctcg ctgatcgccg ccagcaaggc caagatcatt cgcctttcgt
     4201 tccgcgaagc gacggcgatc gacctggccg gtcgcaacgt gctggaggcg gtgcagacga
     4261 gtgtctatcc caaggtcatt gattgcccgc cccgcggcac tcaggccctg gaagccgtgg
     4321 cccgtaacgg cattcagctt cgcgtgcggg cccgcgtgac ggtgcgggcc aacctgcagc
     4381 gggtcatcgg cggggcaact gaagaaacga tcatcgcccg tgtcggcgag ggcatcgtca
     4441 gcgctattgg caaagccgac gaccacatgc aggtgctgga gaaccccgat cagatctcca
     4501 aagaggtgct cgaccggcaa ctcgacgcca atacggcgtt cgagatcgtc tcgatcgata
     4561 tcgcggatat tgacgtggga gagaacattg gagcccggct gcgggccgac caggccgagg
     4621 cggacactcg cgtggcccgt gcccgggccg aaggccggcg ggctgcggcc gtcgccgccg
     4681 agcaggagaa tttcgcccac atcgaagaaa gccgggccaa gctggtcgaa gccgaggccg
     4741 aagtgcccaa ggcgatggcc gactcattcc gatcggggcg gttggggatt ctcgactatt
     4801 acaaactccg caatgtgcaa gccgatacgg agatgcggcg ggccatcgcc ggcgctggct
     4861 cgacgggtgg ctcagcgggg caaggaccgc ggtaaacgcg tgtccaaagt cccatgtcca
     4921 aggtccaaaa ttgttgagac tgaccttgga cgttggactt tggaccttgg accaatccga
     4981 tgcgtgacga agtcgaagaa ttcctccgcc gtgtggctca gcttcgagcc caggccgaag
     5041 cgcaagcccg cgcccagcaa cagcgg
//
LOCUS       DASUGB010001230         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_136214, whole genome shotgun sequence.
ACCESSION   DASUGB010001230 DASUGB010000000
VERSION     DASUGB010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136214"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFV87_23300"
     CDS             complement(<1..293)
                     /locus_tag="VFV87_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5106774.1"
                     /translation="MLTMLGSPRRCCDGVTRRETLKIGALSALGGFGLPQFLAASEAG
                     AVGRGTAGFGMAKSVINIFLLGGAATQDMYDLKPSAPAEVRSQFQPIATSVPGL"
     gene            469..654
                     /locus_tag="VFV87_23305"
     CDS             469..654
                     /locus_tag="VFV87_23305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX5106775.1"
                     /translation="MSDVTHILSKIESSDPAAAEQLLPLVYEELRKLAAAKLAHEKVY
                     ERLKKLCVSMTTGQLVR"
     gene            664..2535
                     /locus_tag="VFV87_23310"
     CDS             664..2535
                     /locus_tag="VFV87_23310"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HEX5106776.1"
                     /translation="MGSPILARTIVVASLLGWAVSARAAEPVSRQMERLGRGLVGIHQ
                     GEGKVFLSWRLLGTDPEGIGFHVDRAVGDGTAERLTDEPLTKVTYFVDSSADLSQPAA
                     YLVTPIVGGREQSASRFELPAEAPPQPYLSVPLQTPEVYRPGDASVGDLDGDGEYEIV
                     LHQVGRGRDNSQAGITTEPILEAYKLDGARLWRINLGKNIREGAHYTQFMVDDLDGDG
                     RAEVACKTADGTIDGKGQVIGEADADHRNSRGYILDGPEFLTVFDGQTGAALATADYI
                     PPRGRVADWGDDYGNRVDRFLACIAYLDGQRPSLVVCRGYYTRAVLAAWNWRDGKLTH
                     VWTFDSDDGTPGNRAYRGQGNHSIAVGDVDDDGRDEIVYGSCVIDDNGRGLYSTGLGH
                     GDALHLADHDPDRPGLEVFKANGDGAHRAGLQMRDARTGEQLWGIASTGRDGVGRACA
                     MDIDPRHRGSEAWGKGGGVGGLFSARGERISDFSPRVCNMGIWWDGDPLRELLDGVSI
                     SKWRWEQGREERIFSGGDYGAVSINGSKSNPCLCADILGDWREELIAATRDGRELRIF
                     TTTIPADRRLVTLMHDPVYRLSIAWQNVAYNQPAHTGFYLGEGMAKPPWPRIMSAGK"
BASE COUNT          494 a          827 c          891 g          467 t
ORIGIN      
        1 agccccggca cgctcgtggc gatcggctga aactggctgc ggacttcggc cggggcgctc
       61 ggcttgaggt cgtacatgtc ctgcgtcgcc gccccgccga gcaggaagat attaatgacg
      121 ctcttcgcca tgccaaatcc agcagtgccg cggccgaccg ctcccgcctc gctggccgcc
      181 agaaactgcg gcagcccaaa gccgccgagt gccgagaggg ccccgatttt gagcgtttcc
      241 cgccgagtga ctccgtcaca acaacgccgc ggactgccga gcatggtgag catggggctg
      301 aagtctcctg gcaggcggga aaggcgggag tcgaaacatc aacggcagtt gatcttcggc
      361 cgaggctcaa gatagcagtc caagatcgac caggcaaact ggattgcgag aataatacaa
      421 gtggcctgct tgtttgcgtt gatatcaagg gctcggcaca atggcggcat gtccgatgtc
      481 acgcacatcc tctcgaaaat cgaatcaagc gatccagccg ccgccgagca acttctgccc
      541 ctggtctatg aagagctgcg gaagctcgcg gcggcaaagc ttgcgcacga gaaggtgtat
      601 gaacgcttga agaagctctg cgtttccatg acgacggggc agttggtcag gtaggaacat
      661 cagatgggta gcccgattct tgctcgaacg atcgttgtgg ccagcctgct tgggtgggcg
      721 gtttcggccc gtgctgctga gcctgtgtcg cggcagatgg agcggctggg tcgcgggctg
      781 gtcggtatcc atcaaggcga aggtaaggtc tttctgagtt ggcggctgct gggcacggat
      841 cccgaaggca ttggctttca cgtcgatcgc gcggtcggtg acggaacagc cgaacgattg
      901 accgatgagc cactgacgaa ggtcacgtac ttcgtcgatt cgtcggccga cctctcgcag
      961 cccgcggcat atctcgtcac gccgatcgtg ggtggccggg agcagtccgc cagccggttt
     1021 gagcttcctg ccgaagcgcc gccacagcca tatctgtccg tgccgctgca aacgcccgag
     1081 gtctatcggc cgggagatgc ttcggtcggc gatctcgatg gcgacggcga atacgaaatt
     1141 gtcctgcatc aggtcggtcg cgggcgcgac aactcgcaag ccggaatcac gaccgagccg
     1201 attctggagg cgtacaaact cgacggcgcc aggctctggc ggatcaacct cggcaagaac
     1261 atccgcgaag gggcgcatta cacgcagttc atggtcgatg acctcgacgg cgacggccgg
     1321 gccgaagtcg cctgcaaaac ggccgacggc acgatcgacg gcaagggtca agtaatcggc
     1381 gaggccgatg ccgatcatcg caatagccgc ggctacatcc tcgatggccc tgaattcctg
     1441 acggtcttcg atggccagac cggcgcggcg ctggcgactg ctgactacat cccgccacgc
     1501 ggccgcgtcg cagattgggg cgacgattat ggcaatcgag ttgatcgatt cctggcgtgc
     1561 attgcgtatc tcgacggcca gcggccgagc ctcgtcgtct gccgcggcta ctacacgcga
     1621 gcggtcctcg ccgcgtggaa ctggcgcgat ggcaagctga cccacgtttg gacgttcgac
     1681 agcgacgacg gcacgccggg caatcgggcc tatcgcgggc agggcaacca cagcattgcg
     1741 gtgggcgacg tcgatgacga cggccgcgac gagatcgtct atggctcgtg cgtgatcgac
     1801 gacaacggcc gcgggctcta ttcgaccggc ttgggtcacg gcgacgcgct gcacctggcc
     1861 gatcacgatc ccgatcggcc cggcctggaa gtcttcaagg ccaacggcga cggggctcat
     1921 cgcgcggggc tgcagatgcg cgacgcccgc accggcgagc agctttgggg catcgcttcc
     1981 accggccgcg acggcgtcgg ccgggcttgc gcgatggata tcgatccgcg tcaccgcggc
     2041 agcgaagcct ggggcaaagg aggcggcgtg ggcggcttgt tcagcgcccg cggcgagcgg
     2101 atttccgact tttcgccgcg cgtctgcaac atgggcatct ggtgggacgg cgatccgctg
     2161 cgggagctat tagacggcgt cagcatttcc aaatggcgct gggagcaggg ccgcgaggag
     2221 cgcattttca gcggaggcga ctacggcgcg gtctcgatca acggttcgaa atccaatccg
     2281 tgtctctgtg ccgatattct cggcgactgg cgcgaggagc tgatcgccgc gacccgcgac
     2341 ggccgcgaac tgcggatttt cacgacgacg atccccgccg atcgccggct ggtgacgctg
     2401 atgcacgacc cagtctatcg actcagcatt gcctggcaaa atgtcgccta caaccagccg
     2461 gcccacacgg ggttctatct gggcgaaggg atggcgaagc cgccgtggcc gcggatcatg
     2521 agcgcgggca agtgagtcat ctggttggtg gtgctcactc gattccccac gccgtcacgc
     2581 cagcctccgt tgcaatcagc agggccggtt tgccatcgag cttcgtcgcc gccagccctg
     2641 tgagggccgc gccgacgtgg aacgaatcat gaaactcgc
//
LOCUS       DASUGB010001231         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_1110776, whole genome shotgun sequence.
ACCESSION   DASUGB010001231 DASUGB010000000
VERSION     DASUGB010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110776"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="VFV87_23315"
     CDS             <1..2206
                     /locus_tag="VFV87_23315"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5106777.1"
                     /translation="MEGDLAAQMVAGIDKFLLREIDLSVERRAKYWKRDFSSPEAYNK
                     SIEPNRQRLAKILGVVDERVKFDAPELIATTRSVSEGRPGLVGKGENYEIFAVRWPAV
                     RGVHGEGLLLVPKGEKVADIIAIPDADQTPEQICGLVEGVPPESQFARRLAENGCRVL
                     VPTLISREMAKRAPPGQPGRANLTNREYLYRPAFELGRHLIGYEVQKVLAGVDWFAKE
                     GGDKEAKIGVMGYGEGGMIALYAGALDTRIGSTCVSGYFGPRERIWEQPIDRNVFGLL
                     EQFGDDELCAMVAPRRLVVDASSYPQVSLPSEGGAPAVLKQDFNEAADVFFKRLGLLS
                     KQWQGKATWLTFTGKPDQAAGTVSGWKLAFPTRPKEIAIAKPVESLRETDVKTRQERQ
                     AHEIDRDTQQILAESPYVREKFMAKLAACKGAEEYSKVVEEYREIFATEVIGRFDNPL
                     LEPNPRSRKSYETEKWVGYEVVLDVFPDVFAYGILCLPKDLKPGEKRPVVVCQHGLEG
                     RPQDTIGEQGSQYYSGFAGKLADRGFVTFAPQNIYIGHDSFRTLQRKANPLGKTLFSI
                     MVPQHQQICNWLKSQPFVDGDRIAFYGLSYGGKSAMRIPPLVKDYCLSICSADFNDWV
                     DKNASTRAPYSYVWTGEYEIFEWDLGSTFNYAEMAALIAPRPFMVERGHFDGVGVDDR
                     VAAEYAKVQRIYNVQLGMPDRCAIEWFVGPHKINGVGTYAFLHKHLNWPEQK"
BASE COUNT          463 a          659 c          741 g          374 t
ORIGIN      
        1 gatggagggg gacctggcgg cccagatggt cgcggggatc gacaagtttt tgctccgcga
       61 gatcgacctg tcggtcgagc ggcgggccaa atactggaag cgcgatttca gctcgcccga
      121 ggcgtacaac aagtcgatcg agcccaaccg ccagcggctg gcgaaaatcc tgggcgtcgt
      181 cgatgagcgg gtaaagttcg acgcgccgga gctgatcgcc acaacccgaa gcgtaagcga
      241 gggacggcca ggtctcgtcg gcaagggaga gaactacgaa atcttcgccg tccgctggcc
      301 cgccgtgcgc ggcgtccacg gcgaaggtct cttgctcgtg cccaagggag agaaggtcgc
      361 cgacatcatc gcgattcccg atgccgatca gacacccgag caaatctgcg ggctggtcga
      421 gggcgtgcca ccggaatcgc aatttgccag gcgcctggcc gaaaacggct gccgcgtgct
      481 cgtccccacg ctcatcagcc gcgagatggc caaacgggca ccacccggcc agccgggacg
      541 ggcgaactta accaaccgcg agtacttgta ccgccccgcg ttcgaattgg gacggcactt
      601 gatcggttac gaagtgcaga aggtgctcgc gggcgtggac tggttcgcta aggaaggggg
      661 cgacaaggaa gcgaagatcg gcgtcatggg ctacggcgaa gggggaatga ttgctcttta
      721 tgccggcgca cttgacacgc gcatcggttc cacctgcgtc agcggctact tcggcccgcg
      781 tgagcgcatc tgggagcagc cgatcgaccg caatgtcttc gggctgctcg agcaattcgg
      841 cgatgatgag ctgtgcgcga tggttgctcc gcgacggctg gtggttgacg ccagcagtta
      901 cccgcaagtg tcgttgccca gcgaaggggg cgccccggcg gtgctgaaac aggacttcaa
      961 tgaggcagcc gacgtgttct tcaaacgtct aggactcctg tcgaaacaat ggcaaggcaa
     1021 ggcaacctgg ctgacattca ccggcaagcc cgatcaggcc gccggaactg tttcgggctg
     1081 gaaactggcc tttccgacca ggcctaagga aattgcgata gcgaaacccg tggaatcgct
     1141 ccgcgaaacg gacgtgaaga ccaggcagga gcggcaggca catgaaatcg accgcgatac
     1201 ccagcagatc ctcgccgaat cgccctacgt ccgcgagaag ttcatggcca agctggcggc
     1261 ctgcaagggg gccgaagagt acagcaaggt ggtcgaggaa tatcgcgaga ttttcgccac
     1321 cgaggtgatc ggccggttcg acaatccgct cctggagccc aatccccgca gccgcaagtc
     1381 gtacgagacc gaaaagtggg tcggctacga ggtggtgctc gatgtgttcc ccgacgtctt
     1441 cgcctacggc atcctctgcc tgcccaagga cctcaagccg ggcgagaagc ggccggtggt
     1501 cgtttgtcag cacggcctgg agggacggcc ccaggacacg atcggcgagc aaggctcgca
     1561 atactacagc ggctttgcgg ggaagctcgc cgatcggggc ttcgtgacgt tcgccccgca
     1621 gaacatttac atcggccacg acagcttccg cacgctccag cggaaggcga acccgctggg
     1681 gaagacgctg ttttcgatca tggtgccgca gcaccagcag atctgcaatt ggctcaagag
     1741 ccagccgttt gtcgatgggg accggatcgc gttttacggc ctgtcgtacg gcggcaaaag
     1801 cgcgatgcgg attccgccgc tggtgaagga ttattgcctg tcgatctgct cggcggactt
     1861 caacgactgg gtcgataaga acgcctcgac gcgagccccg tacagctacg tctggacggg
     1921 ggagtacgag attttcgagt gggacctggg cagcacgttc aactacgccg agatggccgc
     1981 cctcatcgcc ccgcggccgt tcatggtcga gcgggggcac ttcgacggcg tgggtgtcga
     2041 tgaccgggtg gcggccgaat atgccaaggt gcagcggatt tacaacgtgc agctcggcat
     2101 gcccgaccgc tgcgcgatcg agtggttcgt cggcccgcac aagatcaacg gcgtggggac
     2161 ctatgcgttt ttgcacaagc acttgaactg gccggagcag aaatgacgaa tgacgaagca
     2221 cgaatgacga agcacga
//
LOCUS       DASUGB010001232         6512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_136552, whole genome shotgun sequence.
ACCESSION   DASUGB010001232 DASUGB010000000
VERSION     DASUGB010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136552"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFV87_23320"
     CDS             <1..663
                     /locus_tag="VFV87_23320"
                     /inference="COORDINATES: protein motif:HMM:NF015916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HEX5106778.1"
                     /translation="MPATNIPVSLPASVQAATGNQATPVAGTSASPPGAGFSAPGFPN
                     SRFPPADFGSSLPESARPTRERPETFTARRNGPEFPPDTRTAGQAAELTPGLDIWALW
                     GTRVWYRSRVVSLEGDQVRVHYIGWPNASDDTLPIANIRIVVGEAPAANSPAASSAPA
                     AATARTWSDSSGQFKIDAEFVSLEGDMVTLRKTDGKAIRLPIGKLSQADQAVARELAA
                     SQ"
     gene            complement(669..896)
                     /locus_tag="VFV87_23325"
     CDS             complement(669..896)
                     /locus_tag="VFV87_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm17 family immunity protein"
                     /protein_id="HEX5106779.1"
                     /translation="MPIQDIFVGVVTMVLGLALMAGAVLDGAWLMQLARPRFLATAFG
                     KTAARLTLALIGAGLIALGTAVALGWRVDWS"
     gene            complement(907..1701)
                     /locus_tag="VFV87_23330"
     CDS             complement(907..1701)
                     /locus_tag="VFV87_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5106780.1"
                     /translation="MADELYAVVQSKLERIEPAALETALVSGGGIPKPDASRSARKSR
                     GFLAQRISKFQADGIAKQLIAAGHAVRVLPMSQLADVGKPVSVTWLRLTPEALGVPIG
                     AQELVLDVPWSSVFVVNAGHLSVLEQKHREIETRDFRGFPQYETQKISQSERHPAVEV
                     IGLSAEGSLVYLRLISLRMQGTKIPGVPLDLPRHMQFFFVLERLAALSTAAFVSPETR
                     KMLADRKVDRSKTEGSLQFEADERAFTDYSRWLLQLVMFREAEQTR"
     gene            complement(1714..2928)
                     /locus_tag="VFV87_23335"
     CDS             complement(1714..2928)
                     /locus_tag="VFV87_23335"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5106781.1"
                     /translation="MLLSARRYDTLRPVALTVERGRIGRIEPAAHASPPHPPPLSHEG
                     RGEMNAAAADAALPLVAPGLVDLQINGYAGIEFNDPQLTIEKVRQVALSQDRFGVTAF
                     LATCTTDALDVLALSFATIARAIRELPEVAARIPGIHCEGPFISPEDGPRGAHPQQHV
                     RPPNWDEFRRLQDAAEGKIKLLTISPEYDSSADVIRRAADSGVLVAIGHTQATSDQIK
                     AAVDAGARMSTHLGNGAHPQIKRHPNYIWDQLADDRLVASLIVDGHHLPPAVVKSMVR
                     AKTPERIVLVSDITSMGGMSPGKYQTGLGELEVLPSGKLVPAGSPDILAGASLPIDLC
                     VANVMRFAGVDLAAAIDMASTRPAELIGLPNHALEAGSPADLVLFDLPQAAGQPLHVR
                     ATYHGGERTFAA"
     gene            complement(2935..4920)
                     /locus_tag="VFV87_23340"
     CDS             complement(2935..4920)
                     /locus_tag="VFV87_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX5106782.1"
                     /translation="MTSLAIALGAIVLYLVAYHTYGRWLSRKIFRLDPDAVVPSVEVN
                     DNEDYVPTARSIVFGHHFTSIAGTGPIVGPALAIMWGWVPALLWVFFGSIFIGAVHDL
                     GTLVVSLRNRGQTVGDIAGRVLSPRVRLLFLTLLLFALWVVLAIFGWVIANVFIKFPE
                     SILPVFLQIPIAIWIGVRVHKQGKNLLWPSVIALGLMYLTVWFGAACPGMEWTGGWLG
                     SAIQQLNSTLATWPIWAWTGILLIYCYIASVLPVWVLLQPRDYINSLQLISSLGLIVA
                     GLLVAALVGSGGRELTLVAPAVNMHPAGAPPFLPFIFVTIACGAISGFHCLVSSGTSS
                     KQLKNETDAQLVGYGSMLTEGFLATLVIIAVAAGIGLGWPGKYEGAVGTDLWNQVYAN
                     WNGVTGGTAIGAFVVGSANLLEALGINHTMATALMGVLVASFAGTTLDTATRLQRYVV
                     QELAATFAPQVSATACAACGYDLSINIAHCPRCKAPVKGHAPVSHLRSANPLVWLTNK
                     HGATLFAVVTAFILALFPKPGEAWTWETIGTGGLILWPLFGATNQLLGGLAFLVVVFW
                     LWRRKMPVWFAVPPMLLMLVMPAWAMAYDIHRWLLGDKPEYLLGAVAVVMLALEVWMV
                     VEAFLMWPRIRGVLEEALPPLVRPASVPQPAASDSVT"
     gene            complement(5057..>6512)
                     /locus_tag="VFV87_23345"
     CDS             complement(5057..>6512)
                     /locus_tag="VFV87_23345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX5106783.1"
                     /translation="PGQRATATAMAGGSTQVTDVPEVGPMSAVAQAELDHMMPGVGST
                     PMSRQSGDALAVDIEAPAGPGGLGEDYSPEVGISNRQARPDSLNVQLRAARFVKTQVG
                     GLPSVSTAAIVAAEAFSSRGARVRGDQPGSGKGSQSPQTEEAIERGLAFLARYQGAGG
                     GWSLQGFPEGAQLVSDTAATAMAVIAFQGAGYNHREFQYRDAVRGGLDHLIQSQKENG
                     DLFVPLDDASNQSVWLYSHSLATIALCEAYGMTQDPELREPAQKAIDFIIASQHAQRG
                     GWRYAPGVSADTSVSGWMVVAMKTGELAGLEVPTEAWAKVGRWLDDAQQSPAEPHLYR
                     YNPYAPDTLEQRHGRTASKTMTSVGLLMRLYTGWERENPNMIRGADFLLENPPALGTP
                     REPLRDTYYWYYATLVMSHMGGDRWQAWHDRLHPLIVNSQVRQGTYAGSWDPKGAIPD
                     RWGPHAGRIYVTAMNLLSLEVQYRKLRLYEETVK"
BASE COUNT         1151 a         2120 c         2178 g         1063 t
ORIGIN      
        1 atgcctgcga caaacattcc cgtttccctc ccggccagtg tccaagccgc gaccggcaac
       61 caggcgactc cggtcgcggg cacgtcagcc tcgccgccag gcgccggctt cagcgcgccc
      121 ggctttccga attcgcgatt ccctccggct gatttcgggt catcgctgcc ggaaagcgca
      181 cggccaactc gagagcggcc ggaaaccttc acggcccgca gaaatggccc ggagtttccg
      241 ccggataccc gcaccgctgg gcaggctgcc gagttgactc cgggcctgga tatctgggcg
      301 ttgtggggaa cgcgagtctg gtaccggtcg cgcgtcgtga gccttgaagg agaccaggtc
      361 cgcgtgcatt acatcggctg gcccaacgct tccgacgaca cgctgccgat cgcgaacatt
      421 cgcatcgtcg tcggcgaagc ccctgccgct aattcgcccg ctgccagctc cgcgccagcg
      481 gccgcaaccg cgcgcacctg gtccgacagc agcggacagt tcaagatcga cgcggagttc
      541 gtcagcctgg aaggtgacat ggtcacgctc cgcaagaccg acggcaaggc aatccgcctg
      601 ccgatcggca agctcagcca ggccgatcaa gccgtggcgc gcgaactggc agcttcgcag
      661 tgagccgatc acgaccaatc cacccgccag ccaagggcga cggcggttcc gagggcgatt
      721 agtcctgccc cgatgagcgc caaagtcagg cgggccgccg ttttgccaaa agccgtcgcc
      781 aagaagcgcg ggcgggcgag ttgcatcagc caggcgccgt ccagcactgc gccggccatc
      841 aaggccaggc ccagcaccat ggttacgacg cccacgaaga tgtcttgaat cggcatgacg
      901 ggcggattac ctggtctgct cggcttcgcg gaacatgacg agctgcaaca gccagcggct
      961 gtagtcggtg aaggcccgct cgtcggcttc gaactgcagc gagccttcgg tcttcgaccg
     1021 atcgaccttg cggtcggcga gcattttccg cgtttccggg ctgacaaacg ccgccgtgga
     1081 cagcgccgcc agccgctcca gcacgaagaa gaactgcatg tgccgcggta gatcgagcgg
     1141 gacgccggga atcttcgttc cctgcattcg cagcgagatc agccgcagat agacgagcga
     1201 gccctccgca gacaacccga tcacttcgac ggccggatgc cgttcgctct gcgatatctt
     1261 ctgcgtctcg tactgcggaa agccgcggaa atcgcgcgtc tcgatctcgc ggtgcttctg
     1321 ttccaggacc gagagatggc cggcattgac aacgaagaca gaagaccagg gcacgtcgag
     1381 gaccaattcc tgtgcgccga tcggcacgcc gagcgcctcc ggcgtgagtc gcagccacgt
     1441 gaccgagacc ggcttgccga catcggccag ttggctcatc ggcaggacgc ggacggcgtg
     1501 tcccgccgcg atcagttgtt tggcaatgcc gtcggcctgg aacttggaga tccgctgggc
     1561 gaggaatccg cggctcttgc gagcggagcg gctggcgtcg ggcttgggga tgccgccgcc
     1621 ggaaacgagg gccgtttcca gcgccgcggg ctcgatccgc tccagcttgc tttgcacgac
     1681 cgcgtagagt tcgtccgcca tggagagtgc ctcctatgcc gcgaaagtgc gctcgccgcc
     1741 gtgatacgtc gcccgcacgt gaagcggctg gccggcggct tgcggcaggt cgaagaggac
     1801 gagatccgcc ggcgaaccag cttccagcgc gtgattgggc agtccgatca gctccgccgg
     1861 ccgcgtgctg gccatgtcga tcgccgcggc cagatcgact ccggcaaacc gcatcacgtt
     1921 ggccacgcac aagtcgatcg gcagcgaagc gccggccagg atgtccggag agccggccgg
     1981 cacgagcttg ccgctgggca gcacttcgag ttcgccgagg cccgtttgat atttgcctgg
     2041 cgacatgcct cccatcgacg tgatgtcgct caccagcacg atccgctccg gcgttttggc
     2101 ccgcaccatg ctcttcacga ccgcgggggg caggtgatgg ccatcaacga tcaggctggc
     2161 gaccagccgg tcgtcggcaa gctggtccca gatgtaattg ggatgtcgct tgatctgcgg
     2221 atgcgcgccg ttgcccaggt gggtgctcat tcgcgcgccg gcatcgacgg cggccttgat
     2281 ctggtcgctt gtggcctgcg tgtggccgat ggcaaccagg acgccgctat cggccgcgcg
     2341 gcgaatcaca tccgcggagc tgtcgtactc gggcgaaatc gtcagcagct tgattttgcc
     2401 ttccgcggcg tcttgcaggc ggcggaattc gtcccagttc ggcggccgga cgtgctgctg
     2461 gggatgagcg cctcgcgggc catcttcggg agaaatgaac ggcccttcgc aatgaatccc
     2521 gggaatccgc gccgcgacct ccggcagctc gcggatcgcg cgggcgatcg tggcgaacga
     2581 cagcgccagg acgtccagag catcggtcgt gcaagtcgcc aggaaggccg tcacgccgaa
     2641 gcggtcctgc gacaaggcca cctgccgcac tttttcgatc gtgagctgcg gatcgttgaa
     2701 ctcgattccc gcgtagccgt tgatctgcag atcgacgagg ccgggggcga cgagcggcag
     2761 cgccgcgtcc gccgccgcgg cgttcatctc ccctctgccc tcgtgggaga ggggcggggg
     2821 gtgagggggc gaggcgtgag cagccggctc aatccgcccg atccgtccgc gctccacggt
     2881 cagcgcgacc gggcggagcg tatcgtagcg gcgggcggag aggagcatga tggattaggt
     2941 gacggaatcg ctggccgccg gctgcgggac ggaagcgggc cgcacgagcg gcgggagggc
     3001 ctcttcgagc acgccgcgga tacgcggcca catcagaaac gcttcgacca ccatccagac
     3061 ctccagggcc aacatcacca ctgcaaccgc gccgagcaaa tactcgggct tgtcgccaag
     3121 cagccaacgg tggatatcgt acgccatggc ccaggccggc attaccagca tcagcagcat
     3181 tggcggcaca gcgaaccaga cgggcatctt gcgccgccac agccagaaaa ccaccaccaa
     3241 aaacgccagt ccgccgagca actggttggt cgcgccaaac aggggccaca ggatcaatcc
     3301 gccggtgccg atcgtctccc aggtccacgc ctcacctggc ttggggaaga gggccaggat
     3361 gaacgccgtg acaacagcga acagcgttgc gccgtgtttg ttggtcagcc agacgagcgg
     3421 attggccgac cgcagatggc tgacgggcgc gtggcctttc acgggagctt tacagcgggg
     3481 gcaatgggcg atgttgatcg acaggtcgta tccgcacgct gcacaggccg tggcgctgac
     3541 ctggggagcg aacgtcgccg ccaattcctg caccacatag cgctgcagcc gcgtcgccgt
     3601 atcgagcgtc gtgccggcga agctggcgac gagcacgccc atcagcgccg tggccatcgt
     3661 atggttgatg ccgagggctt ccagcaggtt ggccgacccc acgacgaaag ccccgatcgc
     3721 tgtgcctccc gtgacgccgt tccagttcgc gtaaacctgg ttccacaggt cggttccgac
     3781 cgcaccctca tatttgccgg gccagccgag accgattccc gcggcgaccg cgatgattac
     3841 cagtgtcgcc aggaaacctt ccgtcagcat cgagccatag ccgacaagct gcgcgtcggt
     3901 ttcgttctta agttgcttgc tgctggtgcc gctggagacc aggcagtgaa agccgctgat
     3961 cgcgccgcag gcgatcgtca cgaagatgaa cggcaggaag ggcggggcgc cagccgggtg
     4021 catgttcacc gccggggcga ccagcgtcag ttcccgcccg cccgagccga cgagtgccgc
     4081 cacgagcagc cccgcgacga tcaagccgag actgctaatg agctgcaggc tattgatgta
     4141 atctcgcggc tgcagcagca cccaaaccgg gaggacactg gcgatgtagc aatagatgag
     4201 caggatgccg gtccacgccc aaatcggcca ggtggccagc gtgctgttga gttgctgaat
     4261 cgcgctgcct aaccagccgc ccgtccattc cattccgggg caagcagctc cgaaccagac
     4321 cgtcaggtac atcaacccca aggcgatgac cgagggccac aaaaggtttt tgccttgctt
     4381 gtggactcgc acgccgatcc agatcgcaat cggaatttgc aagaataccg gcaggatcga
     4441 ctcaggaaac ttgatgaaga cgttggcgat cacccagccg aaaatcgcca gcacgaccca
     4501 cagcgcgaac agcagcagcg tcaagaacag cagccgcacg cgggggctga ggacgcggcc
     4561 ggcgatgtcg ccgaccgtct ggcctcgatt gcgtaagcta acaaccagcg tgcccaggtc
     4621 gtggacggcg ccgatgaaga tcgaaccaaa aaagacccac aacagcgccg gcacccagcc
     4681 ccacatgata gccagcgccg gccccacaat cggccccgtc cccgcgatcg acgtgaagtg
     4741 gtggccgaag acgatcgacc gggccgtggg gacgtagtcc tcgttgtcgt tcacctccac
     4801 gctcggaacg acggcgtcgg gatcgagccg aaagatcttg cggctgagcc agcggccgta
     4861 ggtgtggtac gcaaccaggt acaggacgat ggcacccagc gcaatcgcca gcgaggtcat
     4921 gacggtaaat ctcccagagg ttcgcccacg tcgcgtttcg caggcttcaa ttctacaagc
     4981 gggcgcgccg cttgtctgcg gcgaacggca atgcatcgct tgccgatcga gtgggccgcc
     5041 gttcgcgcgt caaccgctac ttcaccgtct cctcatacag ccgcagcttg cggtattgga
     5101 cttcgagcga gaggaggttc atggcggtga cgtagatacg gccggcgtgc ggcccccagc
     5161 ggtcgggaat ggcgcctttg gggtcccagc ttccggcgta ggtgccttgg cggacctgcg
     5221 aattgacgat gagcgggtgc aggcggtcgt gccaggcttg ccagcggtcg ccccccatgt
     5281 ggctcatcac gagtgtggcg tagtaccagt agtacgtgtc gcggagcggc tcgcgcggcg
     5341 tgccgagagc tggaggattc tccagcaaaa agtcggcgcc gcggatcatg tttggattct
     5401 cgcgctccca gcctgtatag agccgcatca aaaggccgac gctggtcatg gtcttgctgg
     5461 ccgtgcggcc gtggcgctgc tcgagcgtat cgggggcgta aggattgtag cgatagaggt
     5521 gtggctcggc gggagattgc tgggcgtcgt cgagccagcg gccgaccttg gcccaggctt
     5581 cggtgggcac ttccaggccg gccagctcgc ccgttttcat cgccacgacc atccagccgc
     5641 tgacggatgt gtcggcactg acgccgggag catatcgcca gccgccgcgc tgggcgtgct
     5701 gggaggcgat gatgaagtcg atggccttct gggccggttc gcggagctcg ggatcctgcg
     5761 tcatgccgta ggcttcgcag agggcgatcg tcgccaggct atggctatag agccagacgc
     5821 tctggttcga agcgtcgtcg agcggcacga acaggtcgcc gttttccttt tggctctgga
     5881 tcaggtggtc gagcccgccg cggacggcgt ccctgtactg gaattcgcgg tggttgtagc
     5941 cggcgccctg gaaggcgatg acggccatcg cggtggcggc agtgtcgctg acgagctgcg
     6001 ccccttcggg aaagccttgc aggctccagc cgccgcccgc gccctgatag cgggccagga
     6061 acgcgaggcc gcgctcgatc gcctcctcgg tctgcggcga ctgtgagcct ttgccgctgc
     6121 cgggctgatc gccgcggacg cgggcaccgc ggctggagaa cgcttcggcg gcgacgatgg
     6181 cggctgtgct gaccgagggg aggccgccga cctgcgtctt cacgaagcgg gccgcgcgaa
     6241 gctgcacgtt taggctgtcg ggccgggcct ggcgattgct gatgccaact tccggcgaat
     6301 aatcctcgcc caggccgccc ggaccggcgg gagcttcgat atcgacggcc agggcgtcgc
     6361 ccgactgacg gctcatggga gtgctgccaa cgccgggcat catgtggtcg agttcggcct
     6421 gagcgacggc ggacatcggg ccgacttccg gcacgtcggt cacttgcgtg gagccgccgg
     6481 ccatggccgt ggcggtcgcc cgctggccgg gc
//
LOCUS       DASUGB010001233         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pirellulaceae bacterium isolate SMAG_U1394
            k141_137786, whole genome shotgun sequence.
ACCESSION   DASUGB010001233 DASUGB010000000
VERSION     DASUGB010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961546
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pirellulaceae bacterium (soil metagenome)
  ORGANISM  Pirellulaceae bacterium
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Pirellulaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 06:18:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,672
            CDSs (total)                      :: 4,637
            Genes (coding)                    :: 4,627
            CDSs (with protein)               :: 4,627
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Pirellulaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137786"
                     /isolate="SMAG_U1394"
                     /isolation_source="soil"
                     /db_xref="taxon:2699754"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFV87_23350"
     CDS             <1..298
                     /locus_tag="VFV87_23350"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5106784.1"
                     /translation="HADANLRRCASEVTRPCGVWCAHLTRLKTEPQLALPILEPLKSD
                     GSKYVRDSVGNWLNDASKSQPTWVRQLARRWQKESKTLETAYIVKKALRTMGTK"
     gene            complement(418..1479)
                     /locus_tag="VFV87_23355"
     CDS             complement(418..1479)
                     /locus_tag="VFV87_23355"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX5106785.1"
                     /translation="MKRFQIAVFPGDGIGVEVTAEAVRVLDRVQEIDASFQLDYTHLP
                     WGVEFWKKHGKVVPDDFLSILRPMDAILLGAVGWPAVVPDHLTLSPLVQIRQAFDQYA
                     CVRPSRLFPGVKSVLAGKQPGEIDLVVIRENSEGEYVDNGGRLRRGTPDEVAVQTAVH
                     TRKGVERILRYGFEIARGRRKKLTMVTKSNAQRYAYVLWDETLDRLAPEYPDIATDRQ
                     HCDACVMNFVRWPERFDVVVASNLFGDLLTDLGGIIGGGLGLAPSTNTNPERRFPSMF
                     EPVHGSAPDIAGQGIANPIAAILSAAMLLDHLQLPAAAARIRRAVERTLAEGHQTPDL
                     GGKLTTQQMGQAVVARLAD"
     gene            complement(1476..>2440)
                     /locus_tag="VFV87_23360"
     CDS             complement(1476..>2440)
                     /locus_tag="VFV87_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEX5106786.1"
                     /translation="GVIGIGGSAGTTIGTAAMRALPIGVPKLMVSTLASGNVQPWVGE
                     KDILMLNSVVDIAGINRISRRILGQAAAAMAGMVNFASESNPKSKIQNPKSDRPLIAA
                     TMFGVTTPCVQHARKILEEAGYEVLVFHATGSGGRAMESLIRDGLIAGVLDITTTELA
                     DELVGGILSAGPDRLTAAGQMGIPQVVSVGALDIVNFGPRETVPEKFKDRKFHIHNAS
                     VTLMRTTPEESASLGQEIGRKLAAAKGPAAILLPLQGVSAIDRAGQPFDDPAARAALY
                     DGIRSTRDKTELIELDCHINDPAFADAVTRKLLALLQSSVPLSR"
BASE COUNT          404 a          767 c          850 g          419 t
ORIGIN      
        1 gcacgccgac gcgaacctcc gccgctgcgc cagcgaagtg acgcggccct gcggcgtctg
       61 gtgcgcccac ctcacccgtc tcaagaccga gccacagctc gctctgccga tcctggagcc
      121 gctgaagagc gacggctcga agtacgtccg cgacagcgtt ggcaactggc tcaacgacgc
      181 cagcaaatcg cagcccacct gggtccgcca gctcgcccgc cgctggcaaa aggagtccaa
      241 gacgctcgag acggcctaca tcgtcaaaaa ggccctgcga acgatgggga cgaaatagtc
      301 gtttcgcgag gcacgctgaa cggcgcggga ggcggaggaa ctggccgagc ggccggccga
      361 gatcgaggag atgttaccca ggcggaagcg caagccgaaa gcgccggcta cggggtctta
      421 atccgccagg cgggcgacga cggcctggcc catttgctgg gtggtgagct tgccgccgag
      481 gtcgggagtt tgatggcctt cggccagcgt gcgctcgacg gcgcggcgga tgcgggcggc
      541 cgcggcgggg agctgcagat ggtcgagcag catcgcggcg ctcaggatgg cggcgatcgg
      601 gttggcgatg ccttggccgg cgatatccgg cgccgagccg tggaccggct cgaacatcga
      661 agggaaccgc cgctcggggt tggtgttggt gctgggcgcc aggcccagac cgccgccgat
      721 gatgccgccc aggtccgtga gcaggtcgcc gaacaggttg gaagcgacga ccacgtcgaa
      781 ccgctccggc cagcggacga agttcatcac gcaggcgtcg cagtgctgcc ggtcggtggc
      841 gatgtccgga tactccgggg ccaggcggtc gagcgtttcg tcccacagca cgtaggcgta
      901 ccgctgggcg ttgctcttgg tgaccatcgt cagcttcttc cggcggccgc gagctatttc
      961 gaagccgtag cggaggatcc gctcgacgcc cttgcgggtg tggacggcgg tctgcacggc
     1021 gacttcgtcg ggcgttcccc gcctcagccg gcctccgtta tcgacgtatt ctccttcgga
     1081 gttctcgcga atcacgacca ggtcgatctc gcccggctgc ttgcccgcca gtacgctctt
     1141 cacgcccgga aagagccggc tggggcggac gcaggcgtat tgatcgaacg cttggcgaat
     1201 ctgcacgagc ggcgaaaggg tgagatgatc cggcacgacc gccggccagc cgaccgcgcc
     1261 gagcaggatc gcatccatgg gccgcaggat gctcagaaag tcatccggca cgaccttgcc
     1321 atgctttttc cagaattcga cgccccacgg caggtgcgtg tagtcgagct gaaagctcgc
     1381 atcgatctcc tgcacccgat cgagcacgcg caccgcttcg gcggttactt cgacgccgat
     1441 gccgtcgccg gggaagactg cgatttggaa gcgtttcatc gactgagcgg aaccgaggat
     1501 tggagcaggg ccagcagctt ccgagtaact gcgtcggcga aggcggggtc gtttatgtgg
     1561 caatccagct cgatcaactc ggttttgtcg cgtgtggacc ggatgccatc atagagcgcg
     1621 gcacgggcgg cggggtcgtc gaagggttgg ccggcgcggt cgatcgcgga gacgccttgc
     1681 agcggcagga ggatggcggc ggggcctttg gcagcggcca gtttgcggcc gatttcctgg
     1741 ccgaggctgg cgctttcctc cggcgtcgtc cgcatcagcg tgacactggc gttgtggatg
     1801 tggaacttgc ggtccttgaa tttctccggg accgtctcac gtgggccgaa gttgactatg
     1861 tcgagcgcgc cgacggagac gacctgcgga atgcccatct gcccggctgc ggtcagacga
     1921 tccggcccgg ccgagaggat gccgccgacc aattcgtcgg ccagttcggt cgtcgtgatg
     1981 tcgaggacgc cggcgatcag gccatcacgg atcagcgatt ccatcgcccg cccgccgctg
     2041 ccggtggcgt ggaagacgag gacttcgtag ccggcctctt ccaggatttt gcgggcgtgc
     2101 tgaacgcacg gggtggtgac gccgaacatg gtggcggcga tcagcggccg gtccgatttt
     2161 ggattttgga ttttggattt tggattggat tcagacgcga aattgaccat tcccgccatc
     2221 gcagcggcgg cctggccgag gatgcggcgg ctgatgcggt tgatgccggc gatatcgacc
     2281 accgagttga gcatcaggat gtctttctcg ccgacccacg gctggacgtt gcccgaggcg
     2341 agcgtgctga ccatcagctt cggcacgcca atcggcagcg cccgcatcgc cgccgtgccg
     2401 atcgtcgtcc ccgccgaccc gccgatgcca atcacgccat
//
